LOCUS       DASYSV010000001         3587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_267, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000001 DASYSV010000000
VERSION     DASYSV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VGQ51_00005"
     CDS             <1..432
                     /locus_tag="VGQ51_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034958.1"
                     /translation="PEMGYALNKWLDAGIVVNFTYNSISADPAYNQNISSKEFVYGGG
                     VFARAWFLPFLFVTVQPENNWTSITLKDETYGGTLRQNATAASVLAGIGYGGRGIGQG
                     TFYLALMVDMLRNINSPYNDAYGHPLPVLRAGFDVFVHKQR"
     gene            complement(499..1038)
                     /locus_tag="VGQ51_00010"
     CDS             complement(499..1038)
                     /locus_tag="VGQ51_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV9034959.1"
                     /translation="MEIKCIAVFCGSKFGYDPIFATHTAELGRLIASKNLKVVYGGGS
                     AGLMGLLADTAMAGGASVLGVIPELLMQWEQYHKGITELRTVTDMHVRKKMMYEHCDA
                     AIILPGGFGTLDELFEMLTWNQLRIHSKPIFLLNSGGFYDHLLDHMRHLEKEGFLYDR
                     LDDRIIVCTTPDQIAARLP"
     gene            1068..2594
                     /locus_tag="VGQ51_00015"
     CDS             1068..2594
                     /locus_tag="VGQ51_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV9034960.1"
                     /translation="MSSSLLFVFVIGYFLLLLAVAWRTSRNSNNDSFFIGNRNSNWML
                     VAFGMIGTSLSGITFVSVPGTVGDFAGEGHKAFSYLQVIIGYWIGYLVIAFVLLPLYY
                     RLHLTSIYNYLSSRFGPVAYRTGALFFIISRTVGATARLYVVINVLHIFILRDMGIPF
                     GLTSLVILLMILLYTFEGGVKTIVYTDTLQTTFMLLGLVVCTVYILRHLHFPAGGALH
                     AMGDGGFTSIFNTDVLSRGFFGKQIIGGMFIAIAMTGLDQEMMQKNISVKNLGDSKKN
                     VVTFSFIMVLVSFLFLLLGGLLYLYALDKGAVYQGHTLVLGGKNVIGDDLFPTLALHY
                     LPQSVSIIFIIALISALFPSADGALTALTSSFCIDMLNIKNKGLSEKQLRRTRMTVHG
                     SFAVLFLICILVFKWVDNKSIINVILDLAGYTYGPLLGLFAFGIFTKRLLPNTWKITA
                     LCLLAPVACYFLSEYSARWFGGFQIGIELLVVNGLLTFLGLWGLSRAESRSAVGYVAD
                     "
     gene            2634..3272
                     /locus_tag="VGQ51_00020"
     CDS             2634..3272
                     /locus_tag="VGQ51_00020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV9034961.1"
                     /translation="MEMIDPRAQAYAEKYSSPVGPLLEEIAAYTAANHPEAQMLSGPL
                     QGRLLQLVSTLLQPQRILEIGTFMGYSAICLAAGLGPEGRLHTIELREEDADRAMGYF
                     RRWKWGDRIILHRGNALAILPTLEEVWDIVFIDADKTGYSGYYRQVLPRVRPGGLIIA
                     DNVLFHGQVLEEPIGGKSAKAIQGFNELVAADESVEKVILTVRDGLFFIRKK"
     gene            3279..>3587
                     /locus_tag="VGQ51_00025"
     CDS             3279..>3587
                     /locus_tag="VGQ51_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV9034962.1"
                     /translation="MRIRGIVTGIVLLAAWQRLPAQISSNGVLYVNTYKALAISEEQR
                     SGIPAAIILAQGLHESQAGTSELCKSSNNHFGIKCKDDWRGGVVYHDDDARQECFRSY
                     P"
BASE COUNT          732 a          808 c         1132 g          915 t
ORIGIN      
        1 ccggagatgg ggtatgcgtt gaataagtgg ctcgatgcgg gtatcgtggt gaatttcacc
       61 tataattcga tctcggcgga tcccgcctac aatcaaaata tctcttcgaa agaattcgtc
      121 tatggtggcg gggtttttgc cagggcatgg ttcctgcctt ttttgtttgt gacggtgcaa
      181 ccggaaaata actggacgag tataacgctg aaggacgaga cctatggcgg cacgctcagg
      241 cagaatgcca cggcggcgag cgtgttggcg ggtatcggtt atggcggacg cggcattggc
      301 cagggtacgt tctatctcgc gctgatggtg gatatgcttc gcaatatcaa ctcaccatac
      361 aatgatgcat acgggcatcc gttgccggtg ttgcgggcgg ggttcgatgt tttcgtgcat
      421 aagcagagat agcggcgcgg gtggttagtc gcggcggtcg tttggtcgcg gcggtcgttt
      481 ggtcgcggcg gccgggcgtc agggcagccg ggcggcgatc tggtcgggcg ttgtgcagac
      541 gatgatgcgg tcatcgaggc ggtcgtaaag aaagccttct ttttcgagat ggcgcatatg
      601 atcgagcaaa tggtcgtaga acccgccgga gttgaggagg aagatgggtt tcgagtgtat
      661 cctgagctga ttccacgtca gcatctcgaa caactcgtct agggtgccga aaccgccggg
      721 gaggataatg gcggcgtcgc aatgttcata catcatcttt tttcggacat gcatgtcggt
      781 gaccgtgcga agttccgtga tccctttatg atattgttcc cattgcatga gcagttcggg
      841 gatgacgccc aggactgagg cgccgccagc catggcggta tcggccagaa ggcccatcag
      901 cccggcgctg cctcctccat agacgacctt caggtttttc gaggcgatga gacggcccag
      961 ctcggcggta tgagtggcga agatggggtc gtacccgaat ttcgaaccgc agaaaacggc
     1021 gatgcatttt atctccatag atgggataaa aatacctaat ttcacggatg tcttcatcgc
     1081 tgttattcgt attcgtcatc ggatattttc ttttgttgct ggcggtagcc tggcggacct
     1141 cgcgtaattc caacaacgat tcctttttca tcggaaaccg taattcgaac tggatgctgg
     1201 ttgcattcgg aatgatcggg acctcactat cgggtatcac ctttgtcagc gtgcccggaa
     1261 cggtgggaga ttttgccggc gaggggcata aggcgttcag ctatctgcag gtcatcatcg
     1321 ggtattggat cggctatctt gtcatcgcct ttgtcctgct gcctttatac tacaggctgc
     1381 atctgacctc gatatacaac tatctttcca gcagatttgg accggtggct taccggacag
     1441 gggcgttgtt ctttatcatt tcgcgcactg tgggcgcgac ggcgagattg tatgtggtga
     1501 tcaatgtcct gcatattttc attttaaggg atatggggat tccgttcggt ctgaccagcc
     1561 tggtcatcct gctgatgatc ctgctgtata ccttcgaagg gggtgtgaag acgatcgtat
     1621 ataccgacac gttgcagacg acgttcatgt tactgggact ggtcgtatgc accgtgtata
     1681 tcctgcggca tctgcatttt ccggcgggcg gggccctgca tgccatgggt gacggcgggt
     1741 ttacttccat cttcaatacc gatgtcctga gcaggggttt tttcggcaag cagatcattg
     1801 gcgggatgtt catagcgata gccatgacag ggctggacca ggagatgatg cagaagaata
     1861 tcagcgtgaa gaacctgggg gattccaaga aaaatgtcgt gaccttcagc tttatcatgg
     1921 tgctggtgag ctttttgttc ctgttgctgg gcgggttgtt gtatctgtat gcgctggaca
     1981 agggggcggt ataccaggga catacgctgg tgctgggcgg gaagaatgtc atcggggacg
     2041 acctgtttcc gaccctggcg ttgcattatc tgccacaatc tgtttccatc atctttatca
     2101 tcgcgttgat atcggccttg ttccccagtg cggacggcgc gttgaccgcg ctgacgtcgt
     2161 ctttctgcat cgatatgctg aatattaaaa acaagggact cagcgagaag cagctaaggc
     2221 ggacgaggat gaccgtacat ggcagctttg ccgtcttgtt cctgatctgc atcctcgtat
     2281 tcaagtgggt ggataataaa tccatcatca atgtcatcct cgacctggcg gggtatacct
     2341 atggtccctt attgggtctt ttcgccttcg gtatctttac gaaacggttg cttccgaata
     2401 cgtggaagat cacggccttg tgtctgctgg cgccggtggc gtgctatttt ttatcggaat
     2461 attcggcgcg ttggttcggc gggttccaga tcgggatcga gttattggtg gtcaatgggc
     2521 tactgacctt tttgggatta tgggggttgt cgcgggcgga gagcaggtcg gcagtggggt
     2581 atgtggcgga ttgacctgag gcggccttgc cggattggct tatatttgcg ggcatggaaa
     2641 tgatcgatcc gagagcccag gcctacgcgg aaaaatattc ttcgccggtg gggccgttat
     2701 tggaagagat agcggcgtat acggccgcga atcaccctga ggcgcaaatg ctgagcggac
     2761 cgctgcaagg acggttattg caattggtga gcaccctgct gcagccgcag cggatactgg
     2821 aaatcgggac ttttatgggc tatagcgcga tttgtctggc ggccggattg gggccggaag
     2881 ggcggctgca tacgatcgag cttcgggagg aggatgccga tcgggccatg ggttatttcc
     2941 gccggtggaa atggggggac aggataatat tgcaccgcgg aaacgcgtta gccattcttc
     3001 ccaccctgga ggaggtctgg gacattgtct ttatcgatgc cgacaagaca ggatattccg
     3061 ggtattacag gcaggtgttg ccgagagtgc ggccgggagg gttgatcatc gcggacaatg
     3121 tattgttcca tggacaggtg ctggaagagc cgatcggggg aaagagcgcg aaggcgatac
     3181 agggttttaa tgagttggtg gcagccgatg agagtgtcga aaaagtgatc ctgaccgtta
     3241 gggacggttt attttttatc agaaagaaat aggttttgat gcgaataaga gggatagtga
     3301 cggggatcgt gttgttagcg gcgtggcagc gtttgccggc gcagatcagt tcgaacggcg
     3361 tcttgtatgt caacacctac aaagcgctgg ccatttcgga ggagcagcgt tcgggaatac
     3421 ctgccgccat catccttgcg cagggtctgc atgagtctca ggcgggtacg agcgaattgt
     3481 gcaaatcgtc gaacaatcat ttcgggatca aatgtaagga cgactggagg gggggcgtgg
     3541 tttaccatga cgacgacgcg cgtcaggaat gtttccgcag ctatccg
//
LOCUS       DASYSV010000002        13404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1280800, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000002 DASYSV010000000
VERSION     DASYSV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280800"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VGQ51_00030"
     CDS             complement(<1..1188)
                     /locus_tag="VGQ51_00030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV9034963.1"
                     /translation="MIKHLILPALLLAGVSGFAQEKVVADKIAGIVGDKIILKSELSQ
                     AIGDLTRQNNGVEVPGVTECTIMDQMLLTKALLLQAEKDSLPVSDEEVEAEVDQKIRY
                     FIQLYGGKDAFEQIAQRTVYQAKMDFMAPIREQRLEEAERKKIVEGITITPTEVKDYF
                     DKIPENKRIFYESEMQLNQIVVYPKASHDIEKLAIDELNQYKQQVESGQRKFELLAQL
                     YSDDPGSKDKGGAIELNRNDSKMWDPVFFSTAFRLKEGQVSPVIKSKYGYHIIQMVSR
                     NGDDALVRHILKIPQITQDAIDEATHKLDSVRSQIVDGSLPFGAAVTKYSDDPIAKST
                     AGQIMGKNGTTFLTIPDMDKDMVLLLKNSNLKVGEISKPTVFKDEQGKTGVRIVELVS
                     KSEP"
     gene            complement(1250..4225)
                     /gene="infB"
                     /locus_tag="VGQ51_00035"
     CDS             complement(1250..4225)
                     /gene="infB"
                     /locus_tag="VGQ51_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV9034964.1"
                     /translation="MAETTTLRLMAAAKEFNIGKDTLVDFLVSKGFSKDDLKPTSKLT
                     EEMFRSLQQEFQGDKVAKMKSDQIDLPKGSLEAKKKKEEETLQPLPQQPLFKTKEAPV
                     IVKKPVKEEAPPPVVEVKETPMAPPPVVESVREEPEVTRVETPELEGPKIVSKIDLSS
                     IDSSTRPKKGAKKAGENAAPETQEEAVEPASVQESPRITAKEEAAPNENTGADGSGHV
                     SEPSETGEPETPPVIENIKAEKLEGPKILGKIDLPSTSETRPKPPSEEKRKRKRIPIE
                     KKDQGSGGGRPAGSGPGQYGGGHGGQQGGGQRPGGHQRPPLQRNTDRRGPGGGAGHGH
                     GGGNRRPDPRHAPREAKEIDKKEIQEKIRQTQAKLAGAGGRGKSLKAKYRRAKREEMA
                     EQAGGDEMMDSRLQVTEFISVSELANLMDVSYADVISKCMSLGMMVSINQRLEADVIE
                     LVAGEFGYTVEFIGIDDAAEMEEEEEVDDPEDMMSRSPVVTIMGHVDHGKTSLLDYIR
                     SANVVAGEAGGITQHIGAYQVTTAAGKKITFLDTPGHEAFTAMRARGAKAADVAVIVV
                     AADDAVMPQTKEAISHAQAAGLPMVFAINKIDKDGANPEKIKEQLAGMNLLVEDWGGK
                     YQSQEISAKKGLNVDLLLEKILLEAELLDLKANPDREASGSVIEASLDKGRGYVATVL
                     VQNGTFRIGDLVVSGQYFGRVKAMFNERNKRVDDAPPSTPVLLLGLNGAPQAGEKFKV
                     YEDESDAKEVANKRAQILREQGIRTKKHITLDEIGRRLALGNFKELNLIIKGDVDGSV
                     EALSDSLQKLSTEEIAVRVVHKAVGAITESDVLLATASDAVIVGFNVRPSSQASRLAE
                     NEGVEIKTYSIIYNAIEEVKSAMEGMLEPKIQEKVVANVEVRNVFKFDKATVAGCYVL
                     DGKIFRNSKVRLVRDGIVIYPTGEGATAELASLKRFKDDVKEVPSSMECGLTIKNYND
                     IRVGDIVEAYEEEEVKRTL"
     gene            complement(4240..5487)
                     /gene="nusA"
                     /locus_tag="VGQ51_00040"
     CDS             complement(4240..5487)
                     /gene="nusA"
                     /locus_tag="VGQ51_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEV9034965.1"
                     /translation="MASINLIEAFQDFKEAENIDRPTMMKVVEDVFKTLLRKKYGSDE
                     NFDVIVNAEKGDLEIMRRRTIVEDGNVLDPLAEIGYTDAVKIEPDYEVGEELYEEVEI
                     LDFGRRAILAAKQTLASRIGDLKKNVLVKKYNERIGEIVSAEVYQVWKKEILLLDEEG
                     SELILPKSEQIPQDYFKKGENIRAVVKKVELKNNSPVIILSRTSPLFLSKLLEIEVPE
                     IFDGLIVIKKIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDV
                     INWTNNTQLLIQRSLTPSKITNMELDSEKSHANVYLKPDQVSLAIGRKGVNIKLAQEL
                     TGYTIDVFRDNEGEPEEFDIDLEEFTDEIEPWVIDELKRIGCDTARSVLDLTADELER
                     RTDLEKQTIDDVRRVLKEEFEKG"
     gene            complement(5508..5975)
                     /locus_tag="VGQ51_00045"
     CDS             complement(5508..5975)
                     /locus_tag="VGQ51_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HEV9034966.1"
                     /translation="MVNETIKAVESLVQGLLAGEPGYFLVDVRIKPTNNVKVYIDGDN
                     GISIEKCVQYNRALYKKLEESGLFPSGDFSLEVSSPGLDEPLKLLRQYRKNIGRQVEL
                     VLHDGSKKEGRLMEVSEDGIIVEEVRGKNKKKEVINHTFLFDNIKTTKIQVVF"
     gene            6102..6476
                     /locus_tag="VGQ51_00050"
     CDS             6102..6476
                     /locus_tag="VGQ51_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034967.1"
                     /translation="MSQADNLGSFFAANKTLLKEYLETRLEIYRLQSLRMFARSAGLF
                     VWLLLSLFLAFLFLLFGGMMVAFWFSNLFHSQVKGFGLVAGLILLIFVLLTIFRRALF
                     VEPVIQIVIQKTQEEGKDEEHA"
     gene            6469..6786
                     /locus_tag="VGQ51_00055"
     CDS             6469..6786
                     /locus_tag="VGQ51_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034968.1"
                     /translation="MPKPNQSSRRLDALDEEIRGLREKARELENRIDENLTYFQQHSG
                     SMFVRSLLPRKIEGQDLTGFRLVDRVLENERLQKVLLRLADILAEKAGEGLNWLINKV
                     FKK"
     gene            6804..7031
                     /locus_tag="VGQ51_00060"
     CDS             6804..7031
                     /locus_tag="VGQ51_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034969.1"
                     /translation="MLAEIFLLVIVFYLLYRFVFNFLLPIVKTTRHVRQQFRNMQDSA
                     GEQRQQPKNWQDRHSTTKGAAEDYIEFEDIK"
     gene            complement(7128..7742)
                     /locus_tag="VGQ51_00065"
     CDS             complement(7128..7742)
                     /locus_tag="VGQ51_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV9034970.1"
                     /translation="MKGIKNIIFDLGGVILNLDNRRTEQAFADLGVKDFRQYFGHGHA
                     ASFFMDYEVGKITDRQFIDAIRSLTGPAASDEAIIEGWNALLLDFPPERIGLLKKLRS
                     NYRIFLFSNTNALHLAALRRIYDESFGPGTLEDHFEKTYYSHLLGMRKPDRASFEYIL
                     RENDLKGAETLFVDDAIVNVEGAELAGLKGLYLRPGVSLLDFQW"
     gene            complement(7762..9984)
                     /locus_tag="VGQ51_00070"
     CDS             complement(7762..9984)
                     /locus_tag="VGQ51_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV9034971.1"
                     /translation="MKNIIFAATLLLCLSPAFAQKKTIPQKVDSILRLMTLEEKIGQM
                     NQYNGDWEATGPITNSGDKQDQIRKGMVGSMLNVTGVEHTRQLQEMAMHSRLKIPLLF
                     GQDIIHGYRTTFPIPLAEAASWDLETIQHSARIAALEASAAGVHWTFAPMVDIARDPR
                     WGRVMEGAGEDPYLGSQIAKARVQGFQGKGMGDTNAIMACAKHFAAYGAAIGGRDYNS
                     VDMSLRTLWEIYLPPFKAALDAGAATFMTSFNDLNGIPATGNVYLLRDVLKGHWHFSG
                     FVVSDWGAVGEMIPHGYAKDHAQAAQEAATAGCDMDMESRSYIDNLAKLVRDGKVSLS
                     LIDDAVRRILTKKFEMGLFEDPYRFSNEQRAARQWNNADNLRAGQEMAEKSIVLLKND
                     HQLLPLSRNIGTIALIGPLVRSVRDNLGFWSYQWPDDSARIVTPWMGIRNKLGAGTRL
                     LYAKGCDISDTSRAGFAEAVETAKQADVVIMDVGEAADMTGEAKSHSNIHLPGIQEDL
                     IRAIEATGKPVVVMISAGRPLIFNWTADHVPAILYTWWLGTEAGNAMADVLFGDYNPS
                     GKLPMSFPRTEGQIPIYYDHFNTGRPAKNDSDVNYVSAYLDLPNSPRFPFGYGLSYTS
                     FSYSPITLSASAFRPGGSVTATVTVTNTGKYDGRETVQWYIRDMFGSVVRPVKELKGF
                     EQVFIKAGQSQKLTFTIHADDLRFYNEKLEYRFEPGEFRLFVGGNSKDVQQAAFTLDD
                     "
     gene            complement(9981..12266)
                     /gene="bglX"
                     /locus_tag="VGQ51_00075"
     CDS             complement(9981..12266)
                     /gene="bglX"
                     /locus_tag="VGQ51_00075"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HEV9034972.1"
                     /translation="MRITLTIVAFLLCLSATKAQSPAGGGDAKMNAFISQLMSKMTLG
                     EKIGQLNLPGAGDITTGQAANSGIAGKIEKGEVGGLFNIKTVAKIKDVQKLAVEKSRL
                     KIPLIFGMDVIHGYETVFPIPLALSCSWDMSEIEESARIAAKEASADGICWTFSPMVD
                     ICRDPRWGRIAEGGGEDPYLGSQIASAMVKGYQQDLSGNSDIMACVKHYALYGAAEAG
                     RDYNTTDMSLVRMFNEYLPPYKAAVDAGVGSVMSSFNDVNGIPATANRFLLTDVLRRR
                     WGFKGFVVTDYTGINEMVAHGMGDLQTVSALALKAGVDMDMVGEGFLTTLKKSLSQGK
                     VTAAQIDLACRKVLEAKYKLGLFANPYKYCDESRAANDIFTFANRTAARRIAADCFVL
                     LKNQKDVLPLRKGGTIAVIGPLGDNKENMPGTWSVAADFSKSVSLLQGMQDAVGENGK
                     IIYSMGSNLDADSLFEERAGMFGKDLHRDHRSAEAIIRDALDVASKADVIVAALGESA
                     EMTGESSSRSMIGIPGVQEDLLKALLATGKPVVLVLFTGRPLTIKWENDNVPAILNVW
                     FGGTEAGHAIADVLFGDVNPGGKLTATWPQNVGQIPLYYNHMNTGRPLEGPWFQKFRS
                     NYLDVSNDPVYPFGYGLSYTHFTYSDLTLSATKLKGAQKLTATVTVTNDGNRPGKEVV
                     QLYTRQLVGSITRPVEELKGFQKIDLKTGESRKVSFTITPEDLKFYNNDLKYDWEAGD
                     FVIMIGGNSRDVLSAKVNWTK"
     gene            complement(12279..>13404)
                     /locus_tag="VGQ51_00080"
     CDS             complement(12279..>13404)
                     /locus_tag="VGQ51_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV9034973.1"
                     /translation="QRFGEYMDNTFLDPFIEGSWMTKHDGKYYLQYGAPGTEFSGYAD
                     GVVVGDGPLGPFDKPQSDPFSFHPGGFARGAGHGSTFTDNAGHYWHISTITIAVKNSF
                     ERRLGIWPAGFDKDGVMYMDASFGDYPHYVPSGRFTGWMLLNYKKPVLVSSTLSPAHP
                     PNDAVDEDIKTYWSAATGGKGEWIQSDLGAVSTVYAVQINYADQDAEFLGKQTDIYHQ
                     YRLLGSTDQQHWRVLIDKGHNTTDVPHDYVQLETPANVRYIRLENIHMPTGKFAVSGL
                     RVFGKGTGPLPDTVKDLIVLRTGKDKRSAWLKWGPVTNASGYNIYYGTSPDKLYTCIQ
                     VNDDNQYWLKTMDKEKIYYFAAESFNENGVSPRTAVIKAD"
BASE COUNT         2725 a         3650 c         3477 g         3552 t
ORIGIN      
        1 cggttccgat ttggatacca attcgacgat cctgacgcct gttttgccct gttcgtcttt
       61 aaatacagta ggcttggata tttcgccgac tttgagattg gaattcttca gtaacaacac
      121 catatccttg tccatatcgg gaattgtcag aaaggttgtc ccattcttgc ccatgatctg
      181 gcctgcggtg gatttggcga tcggatcgtc gctatatttt gtcacggccg cgccgaaggg
      241 aagagaaccg tctacgatct gcgagcggac cgagtccagt ttatgggtag cctcgtcgat
      301 ggcatcctgg gtgatctgcg ggatcttcag gatatgcctg accagcgcat cgtcgccgtt
      361 cctgctcacc atctggatga tatgataacc gtatttgctt ttgatgacgg gagagacctg
      421 tccttccttc aaccggaatg cagtggagaa gaagacggga tcccacattt tgctgtcgtt
      481 acggttcagt tctatggctc cgcctttgtc cttgctgccg gggtcatccg aatagagctg
      541 tgccagcaat tcgaatttac gctgaccgct ctctacctgc tgtttgtatt gattcaactc
      601 gtcgatcgcg agcttctcga tatcgtggct cgctttggga taaacgacta tctggttcag
      661 ctgcatttcg gattcataaa agatccgttt gttctcgggt atcttatcga aataatcttt
      721 gacttccgta ggggtgatgg taataccttc gacgatcttt ttccgctcgg cctcctccag
      781 ccgctgttcg cggataggag ccatgaaatc catcttcgcc tggtagaccg tccgctgggc
      841 aatctgctcg aaggcgtcct tcccgccata tagctggatg aagtaccgga tcttctgatc
      901 tacctccgct tccacttctt cgtcgctgac gggcagggaa tccttttccg cctgcaatag
      961 cagcgctttt gtcaggagca tctggtccat gatcgtgcat tccgttacgc ccgggacttc
     1021 cactccgttg ttttgacggg tcagatcgcc tatcgcctga gaaagctcgg atttgagaat
     1081 gatcttatcg cctacgattc cggcaatctt gtcggcaaca actttctcct gtgcgaaacc
     1141 ggaaacgccg gcaagcagca gcgcaggcaa tatgagatgc ttgatcatat gtcaaaagta
     1201 taatgcgaaa tatgttaaat acccccgtgc gggggtattt aacaaaaaat tagagcgttc
     1261 gttttacttc ttcttcctca taggcttcga cgatgtctcc tacgcggata tcattgtaat
     1321 tcttaatggt aagaccgcat tccatgctgg aggggacttc ttttacgtcg tccttgaacc
     1381 gtttgagcga tgccagctca gcagtagcac cttctccggt gggatagatg acgatgccat
     1441 cgcggaccag gcggaccttg gagttccgga agatctttcc atcgagcaca tagcaacctg
     1501 cgacggtcgc cttgtcgaat ttgaagacat tgcgcacttc gacgttggcc accactttct
     1561 cctggatctt cggttcgagc atgccttcca tagcggactt gacttcttcg atggcattgt
     1621 aaatgatcga ataggtcttt atttccactc cttcattttc agccaggcgg gaggcctgcg
     1681 aggacgggcg gacattgaag ccaacgataa cggcgtcgga ggcggtagcc agcaggacat
     1741 cgctttcagt gattgcgccg acggctttat gtacgacgcg tacggcgatc tcctctgtag
     1801 agagtttctg cagtgagtca cttagtgctt ccacggatcc atccacatcg cccttgatga
     1861 tgaggttgag ttccttgaag ttgcccagcg ccagacggcg gccgatctca tcgagcgtga
     1921 tatgtttctt ggtgcggatg ccttgttcgc gcaggatctg ggcgcgtttg ttcgccactt
     1981 ccttggcatc gctttcgtct tcgtagacct tgaatttctc accggcctgc ggagccccgt
     2041 tgagacccag caacagaaca ggggtggaag gcggagcgtc gtccacgcgt ttgttccttt
     2101 cgttgaacat agcttttaca cggccgaaat attgaccgga tacgacgagg tcgccgatac
     2161 ggaaggtacc attctgtacc agtacggtag ccacatagcc gcgtccttta tccagggatg
     2221 cttcgataac gctgccggag gcttctcgat cgggattagc cttgagatcg agcagttcgg
     2281 cttccagcag tatcttttcc agcaggaggt ctacattcaa tccttttttc gccgatatct
     2341 cctgggactg atatttaccg ccccagtctt cgaccaggag gttcataccc gcaagctgtt
     2401 cttttatctt ttcggggttt gctccgtctt tatcgatctt gttgatagca aaaaccatgg
     2461 gcagacccgc tgcctgggcg tgcgagatcg cttccttggt ctgaggcatc actgcgtcgt
     2521 cggctgcaac aactatgact gctacgtcgg cggctttggc accacgcgca cgcatggccg
     2581 taaaggcttc gtgaccggga gtatccagaa aggtgatctt tttgcctgcc gcggtagtca
     2641 cctggtaggc gccgatatgc tgggtgatac cgcctgcttc gccggcgacc acatttgcgc
     2701 tgcggatata atcgagcagc gaggtcttac cgtggtcgac gtgacccatg atggttacga
     2761 caggagaacg ggacatcata tcttccggat cgtccacttc ttcttcttcc tccatttcgg
     2821 cggcatcatc gatgccgatg aattcgaccg tatatccgaa ctcacccgca accaactcga
     2881 tgacatcggc ttccagacgc tggttgatgg acaccatcat tcccaggctc atacacttgc
     2941 tgatgacgtc ggcatagctg acgtccatga gattagccag ctcgctgacg ctgatgaatt
     3001 cggtgacctg caaacggctg tccatcatct cgtcgcctcc ggcctgttcg gccatctctt
     3061 cccgtttggc ccggcgatat ttagccttga ggctcttccc gcggccgccg gcgcctgcca
     3121 gcttcgcctg tgtctgcctt atcttttcct gtatctcttt cttgtcgatc tcttttgcct
     3181 cacgtggtgc atgacggggg tcgggccggc gattgccgcc tccatgaccg tggcccgcgc
     3241 ctccaccagg tccgcgacga tccgtgtttc gttgcaatgg gggccgctga tgcccgccgg
     3301 ggcgctgacc gcctccttgc tgaccgccgt gaccaccacc gtattgtccc ggaccgcttc
     3361 cggcgggtct tccgccaccg ctaccctgat cctttttctc gatgggtatc cttttgcgtt
     3421 ttctcttttc ttcggatggg ggtttggggc gggtttcact cgtggatggc agatcgatct
     3481 ttccgaggat cttggggcct tccagctttt ccgcctttat attttcgatg accgggggtg
     3541 tctcgggttc acctgtttcc gatggttcag agacatgacc ggaaccgtct gcgccggtat
     3601 tttcattcgg cgccgcttct tctttagccg tgatgcgggg gctttcctga acggacgcgg
     3661 gttcgacggc ctcttcctgt gtttcggggg cggcgttctc acccgccttt ttcgcgcctt
     3721 ttttgggccg tgtggacgag tcgatggagg acagatcgat cttgctgacg atcttaggtc
     3781 cttcgagctc aggggtttcc actcttgtta cctctggctc ttcgcgcacg gattccacca
     3841 ccggcggcgg tgccatgggc gtctctttca cttccacgac ggggggaggc gcttcttctt
     3901 ttaccggctt ctttacgata acaggcgctt ccttggtttt gaatagcggc tgctgcggta
     3961 gtggctgcag ggtctcctct tcctttttct tcttcgcctc caagctgccc ttgggcaggt
     4021 cgatctggtc ggatttcatt ttggcgacct tatctccctg gaattcctgc tgtaaagaac
     4081 gaaacatttc ctccgtcaac ttggacgtag gcttcagatc gtctttgctg aatcctttgc
     4141 tcacaaggaa atccaccagg gtatctttcc cgatgttaaa ttccttggct gcagccatta
     4201 ggcgtaatgt cgttgtttct gccataaatt atttaactgt tatccttttt caaactcttc
     4261 cttcagcacc cggcgaacat catcgatggt ctgtttttcc agatcggtcc ttctctcgag
     4321 ttcgtctgct gtaaggtcga gcacgctccg ggccgtatcg cagccgatcc gtttcagctc
     4381 gtcgatgacc cagggctcga tctcgtcggt aaattcttcg aggtcgatat cgaattcttc
     4441 cggctcgccc tcgttatcgc ggaatacgtc gatcgtatat ccagtcaatt cctgcgccag
     4501 cttgatattg acccccttgc gcccgatggc cagggaaacc tggtccggct ttaaatagac
     4561 attcgcatgg gacttttcgc tgtccagctc catatttgtg atcttggacg gggtcagcga
     4621 gcgctggatc agcagctggg tgttattggt ccagttgatg acgtcgatat tttcgttctt
     4681 cagctcgcga acgataccat ggatacggct gcccttcatc ccgacgcagg ctccgacggg
     4741 gtcgatccgg tcgtcatagg attccacggc taccttcgcg cgttcgcccg gttcgcggac
     4801 gatctttttg atgacgataa ggccatcgaa aatttccggc acctctattt ccagcaattt
     4861 ggagaggaat agcgggctgg tccgggatag gatgatcaca ggcgaattgt ttttcaattc
     4921 tacctttttc accacggccc ggatgttctc cccttttttg aaataatcct gcgggatctg
     4981 ttcggatttg ggaaggataa gctcgctacc ctcttcatcc agcagaagga tctccttctt
     5041 ccagacctgg tagacttcgg cgctgacgat ctccccgatc ctttcgttgt acttttttac
     5101 caatacgttc tttttcagat cgccaatgcg gctggccagc gtttgtttgg cggccaggat
     5161 cgcgcgacga ccgaaatcca ggatctccac ttcttcatac agctcctccc caacctcata
     5221 atcgggctca attttgacgg cgtcagtata accgatctcc gccaggggat ccaacacgtt
     5281 cccgtcttcc acaatggtac gacgacgcat gatctccagg tcacccttct ctgcgttgac
     5341 aatgacgtca aaattttcgt cgcttccata tttcttgcgc aacagggtct tgaagacgtc
     5401 ttctactact ttcatcatag taggacggtc gatattttcg gcttctttga agtcctggaa
     5461 tgcttcgatc aaattgatac tggccataac actgaatttt agaaactcta gaaaactact
     5521 tgaatttttg tcgtttttat gttatcaaat aaaaaagtat ggttgatcac ctcttttttc
     5581 ttgttcttac ccctgacttc ctccacaatg atgccatctt cactcacttc catcaaacgg
     5641 ccttcttttt ttgagccgtc gtgcagtacc agctccactt gcctgccgat attcttccgg
     5701 tattgacgca gcagtttcag cggctcgtcc aatcccggag atgaaacttc cagggaaaaa
     5761 tcacccgaag gaaaaagacc cgactcttcg agctttttat acagcgcgcg gttatactgc
     5821 acgcactttt cgatggagat gccgttgtcc ccatctatat atactttgac gttattggtg
     5881 ggcttaatcc ggacgtctac caggaaatat ccgggttctc ccgccagcag accctgcacc
     5941 aaactttcta cagctttgat tgtttcgttg accatggtcc taagaaataa agaagggaac
     6001 gagcccgttc ccttttcttt tgcttcattt gccgttgcaa atatacaata atcgatggct
     6061 caacgaaaaa ataattttta tacttttagc aaaacatcca tatgagccag gccgataatt
     6121 tgggaagttt tttcgccgca aacaaaactt tactgaaaga atatctcgaa acccgactcg
     6181 agatctatcg tcttcagagt ctccggatgt tcgccagatc ggccggtctt tttgtgtggt
     6241 tgctcctgtc gctgtttctg gcgttcctct ttttgctttt tggcggaatg atggttgcat
     6301 tctggttttc caaccttttc cacagccagg taaaagggtt cgggttggtg gccgggctga
     6361 tcctgctgat cttcgtgtta ttgaccattt tcaggcgagc cttatttgtg gagcctgtta
     6421 ttcaaatagt tatacaaaaa acccaggagg aggggaaaga cgaggaacat gcctaaaccc
     6481 aaccaatctt cccggcgcct ggatgcgctg gatgaggaga tccgcggact ccgcgagaaa
     6541 gcgcgggagc tggaaaaccg gatcgatgaa aatttaactt atttccagca acattccggc
     6601 tcgatgttcg taagatcatt gttacctcgt aagatcgaag gtcaggacct gaccggcttc
     6661 cggctggtgg accgggtgct ggagaacgaa cggctgcaaa aggtactttt acggctggcc
     6721 gatattctcg ccgaaaaggc cggagaaggt cttaactggt tgattaacaa agtatttaaa
     6781 aaataattta tctttgccgg attatgttag ccgagatatt tcttctggtc atcgtgtttt
     6841 acctgctgta tcgatttgtg ttcaattttc ttttgcccat cgtcaagacc acccgtcatg
     6901 taaggcaaca attcaggaat atgcaggatt cagcgggaga gcagcggcag cagcctaaaa
     6961 actggcagga tcgccattct actacgaagg gggccgcgga ggattatata gaattcgagg
     7021 acatcaaata actgaggcgc ccacggggcg cctcgtcaga gctgcctcgc tgcgctagac
     7081 cttcgtcgaa gcgtcgtcca tcgcttctcc tcggtcgtag gctacgccta ccactgaaaa
     7141 tccaacagac tgacgccggg ccgcagatat agtcccttta gcccggccag ctccgcgccc
     7201 tccacattca cgatggcatc atccacgaaa agcgtctccg cacctttgag atcattttcc
     7261 ctcaatatat attcaaaaga cgcccggtca ggcttccgca tgcccagcaa gtgcgaataa
     7321 taggtctttt cgaaatggtc ttccagcgta ccggggccga atgattcgtc atagatccgc
     7381 cggagggcag cgagatgcag cgcattggtg ttgctgaata aaaagatccg gtaattgctg
     7441 cgcagctttt tcaacaggcc gatccgctcc ggcgggaagt ccagcagcaa tgcattccag
     7501 ccttcgatga tggcctcatc ggaggcagcc ggacccgtaa ggctcctgat ggcgtcgatg
     7561 aattgccggt cggtgatctt accgacctca taatccatga aaaacgaggc ggcatgtcca
     7621 tgaccgaaat attgccggaa atctttcact ccaagatcgg caaaggcttg ctccgtgcgc
     7681 cggttgtcca gatttagtat aactccccca agatcgaaga tgatgttttt gatgcccttc
     7741 ataagcgatc aaatataaag cttaatcgtc cagcgtaaat gccgcttgtt gaacatcctt
     7801 cgagtttccg ccgacaaata atctgaattc accgggctcg aagcgatact ccagcttttc
     7861 attatagaac cgaagatcat cggcatggat cgtaaacgtg agcttctgcg attgtcccgc
     7921 ttttatgaac acctgctcga atccttttag ttcttttacc ggtcggacca ccgagccgaa
     7981 catatcgcgg atataccatt gcaccgtttc cctgccatca tatttgccgg tattcgtgac
     8041 cgtgaccgta gccgtaaccg aaccaccggg tctgaacgcg gaagcgctca gtgtaatggg
     8101 gctatatgaa aagctcgtat agctgagccc ataaccgaaa gggaaccggg ggctgttggg
     8161 cagatcgaga tatgcggaga catagttgac gtccgagtcg ttttttgcgg ggcggccggt
     8221 gttgaaatga tcatagtaga tgggtatctg gccttccgta cgcggaaagc tcatcggcaa
     8281 tttacccgaa gggttatagt caccgaacaa tacgtcggcc atggcattgc ctgcctcggt
     8341 gccgagccac caggtgtaca ggatcgcggg gacgtgatcc gccgtccagt tgaagatcag
     8401 cggcctgccc gcgctgatca tgacgaccac gggtttgcct gttgcctcga tagcccggat
     8461 gaggtcttcc tgtatgccgg gcagatggat attgctgtgg ctcttggctt caccggtcat
     8521 gtcggctgcc tcgccgacat ccatgatcac gacatccgcc tgtttcgcgg tctctaccgc
     8581 ttccgcaaaa cccgcgcggg aggtgtcgct gatatcacag cctttggcat agagcagtct
     8641 tgttccggct ccgagtttat tgcggatgcc catccacggg gtgacgatcc ttgccgagtc
     8701 gtcgggccat tgataggacc agaaacccag gttatccctg accgatcgga ccagcggtcc
     8761 gatgagggcg atcgtcccga tattcctgga cagcggcagc aactgatgat cgttcttcag
     8821 caatacgatg cttttctcgg ccatctcctg gccggcccgc aggttgtcgg cgttgttcca
     8881 ttgccgtgcg gcgcgttgtt cgttactgaa ccggtatggg tcctcgaaaa gccccatctc
     8941 gaattttttc gtcaggatac ggcggacggc gtcgtcgatg agagaaaggg atactttccc
     9001 gtcacggacc aatttggcca gattgtcgat atagctacgg ctttccatgt ccatgtcgca
     9061 accggcggtg gcggcttcct gcgcggcctg ggcgtggtct ttggcatagc catgcgggat
     9121 catttctcca accgcgcccc agtcgctgac gacaaagccg gaaaaatgcc aatggccttt
     9181 cagcacatcc cggagcagat agacgttgcc ggtagcgggg atgccgttga gatcgttgaa
     9241 agaggtcata aaggtggccg cgccggcatc caaagcggcc ttgaatggcg gaagatagat
     9301 ctcccacagc gttcgcagac tcatgtctac cgagttgtaa tcacggcctc cgatggccgc
     9361 tccatatgcc gcgaaatgtt ttgcgcaggc catgatggca ttcgtgtctc ccatgccttt
     9421 tccctgaaaa ccttgcacgc gggctttcgc gatctgtgaa cccagatagg ggtcctcccc
     9481 tgccccttcc atcaccctgc cccaccgcgg gtcacgggca atgtctacca tcggggcgaa
     9541 agtccaatgc acgccggctg ccgaggcttc gagggcggcg atccgggctg aatgctgtat
     9601 cgtctccaaa tcccaggaag cggcctccgc gagcgggatg gggaatgtag tcctgtagcc
     9661 gtgaatgatg tcctggccga atagcagcgg tatcttcagc cgggagtgca tagccatttc
     9721 ctgaagctgc cgggtatgtt ccaccccggt gacattcagc atggagccga ccatgccttt
     9781 gcggatctgg tcctgtttgt cacccgagtt agtgatgggg ccagtcgctt cccaatcgcc
     9841 gttatactgg ttcatctgcc cgatcttttc ttcgagcgtc atgagccgca atatggaatc
     9901 caccttctgc gggatggtct ttttttgggc aaatgccggg gaaagacaga ggaggagcgt
     9961 ggctgcaaaa attatgtttt tcatttcgtc cagttaactt tcgccgaaag gacatcgcgg
    10021 gaattgccgc caatcatgat cacaaaatcg cctgcctccc aatcgtactt aagatcgttg
    10081 ttatagaatt tcaggtcttc gggagtgatg gtaaagctca ctttccgcga ttcgcccgtc
    10141 ttaagatcga tcttctgaaa tccctttaat tcttctacag gtctggtaat gctgcccacc
    10201 aattggcggg tatacaattg taccacttct ttgcccgggc ggttgccgtc attggtgacg
    10261 gtgacggtcg ccgtgagctt ctgagcgcct tttaatttcg tagcggataa cgtcagatcg
    10321 ctgtaggtga agtgcgtata gctgagtcca taaccaaagg gatagacggg gtcattggaa
    10381 acgtccaggt agttggaacg gaatttctgg aaccagggtc cttccagtgg tcggcccgtg
    10441 ttcatatgat tatagtataa cgggatctgt ccgacatttt gcggccacgt ggcggttagc
    10501 tttcccccgg gattgacgtc gccgaaaagc acatcggcta tggcgtgtcc tgcctccgtc
    10561 cctccgaacc agacgttgag gatggcgggg acattgtcgt tctcccattt gatggtcagg
    10621 gggcgaccgg tgaaaagaac cagcacgacg ggcttacctg tggccagcaa ggctttcagc
    10681 agatcctcct gcactcccgg aataccgatc atgctacggc tgctggattc gccggtcatc
    10741 tccgcggatt ctcccagcgc ggcgacgatc acatccgcct tcgaagctac gtcgagcgcg
    10801 tcccggatga tggcttccgc ggaccggtga tcgcgatgca ggtctttgcc gaacataccc
    10861 gccctttctt cgaaaagact gtcggcatct agattggaac ccatgctgta gatgatcttt
    10921 ccattttcgc cgacggcgtc ctgcatgccc tgcagtaaag aaacggactt gctgaaatcg
    10981 gctgctacgc tccaggtacc cggcatattc tctttattgt ctccaagggg tcctataacc
    11041 gcgatggtgc ctccttttct caggggtagt acgtccttct ggttcttcag cagtacgaaa
    11101 cagtcggcgg cgatgcgtct cgcagccgtc cggttggcga aagtgaagat atcgtttgcg
    11161 gcgcggctct catcgcagta cttataggga ttggcgaaga gtccgagttt atatttcgct
    11221 tccaatactt tccggcatgc aagatcgatc tgcgccgccg tcaccttacc ctggctgagc
    11281 gatttcttta aggtggtcag gaatccctca ccgaccatat ccatatcgac gcccgccttc
    11341 agcgccagcg cggagactgt ctgaagatcc cccattccat gtgcgaccat ctcattgatg
    11401 ccggtataat ccgtgaccac gaatcccttg aatccccaac gacgacggag gacatcggtc
    11461 agcaggaacc tgttggctgt ggcgggaatg ccgttgacgt cgttgaacga ggacatgacg
    11521 ctgcctacac ctgcgtcgac cgccgcctta tagggcggaa gatattcatt gaacatcctg
    11581 acgaggctca tgtccgtagt attgtaatct cgtccggctt cggcggcgcc gtaaagcgca
    11641 tagtgtttga cgcaagccat gatatcgctg ttcccggaaa ggtcctgctg gtatcccttt
    11701 accatcgcgc tggcgatctg tgagcccagg taagggtctt caccgccgcc ttcggctata
    11761 cggccccaac gggggtcgcg gcagatatcc accatcgggg agaaggtcca gcagatgccg
    11821 tcggcgctgg cttctttggc ggcgatgcga gcgctctctt ctatctcgga catatcccag
    11881 ctgcagctga gggcaagcgg tatcgggaaa accgtttcat atccatggat gacgtccatt
    11941 ccaaagatga ggggtatttt cagccgactt ttctcgacgg caagtttttg tacatccttg
    12001 atctttgcaa cggtcttgat attgaacaaa ccgccgacct cgcctttttc gatctttccc
    12061 gcgatgcctg aattggcggc ctgacccgtc gtgatgtcgc cggcgcccgg gagattcagc
    12121 tgtccgatct tttcgccgag cgtcattttg ctcatcagct gactgataaa agcgttcatt
    12181 ttggcgtctc cgccgccggc gggcgattga gccttcgtag ctgagagaca gagaagaaag
    12241 gcaacaattg ttagggttat tctcatatga tgtagttatt agtccgcctt gatcacagcc
    12301 gtgcgcggag agacaccatt ctcattgaag gactctgccg caaaataata gattttttcc
    12361 ttgtccatgg tcttcagcca atattgattg tcgtcattca cctggataca ggtatataat
    12421 ttatccggtg acgtgccgta gtaaatatta tacccggagg cgttggtcac cggaccccat
    12481 tttagccagg cgctgcgttt gtcttttccg gtgcgcaaga cgatgaggtc tttgacggtg
    12541 tccggaagcg gtccggttcc tttgccgaag acgcgtaaac cgctgaccgc gaatttgcct
    12601 gtcggcatat gaatattctc caggcggatg tagcggacat tcgcaggtgt ttccagctgt
    12661 acatagtcat gaggaacgtc ggttgtgtta tggcctttgt cgatgagaac ccgccaatgt
    12721 tgctggtcgg tagagcccag cagccggtat tgatgataaa tgtccgtctg tttgcccagg
    12781 aactccgcat cctgatcggc atagttgatc tggacggcat atacagtcga gacggccccg
    12841 agatcggatt ggatccattc tcccttgccg ccggtggctg cgctccaata ggtcttgatg
    12901 tcttcgtcta ctgcgtcgtt tggcggatgc gcggggctta gtgtcgagga gacgaggacc
    12961 ggctttttat aattcagcag catccagcct gtaaaacggc cgctgggaac ataatgcgga
    13021 taatcgccga aagatgcgtc catatacatc actccgtcct tgtcgaaacc cgcgggccag
    13081 atgcccaatc gtcgctcgaa gctgttcttg acagcaatgg tgatggtgga aatatgccag
    13141 taatggccgg cgttgtcggt gaaggtgctc ccatgtcccg ccccccgcgc gaatccaccc
    13201 gggtgaaaag aaaacggatc ggactgaggt ttatcgaaag gtcccaatgg tccatcgcca
    13261 acgactacgc catctgcgta gccgctaaat tccgtgccag gcgcaccata ttgaagatag
    13321 tatttcccgt cgtgtttggt catccatgaa ccttctataa acggatcgag gaaagtattg
    13381 tccatgtatt ctccgaaccg ttgc
//
LOCUS       DASYSV010000003         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2177529, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000003 DASYSV010000000
VERSION     DASYSV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177529"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /gene="traM"
                     /locus_tag="VGQ51_00085"
     CDS             <1..478
                     /gene="traM"
                     /locus_tag="VGQ51_00085"
                     /inference="COORDINATES: protein motif:HMM:NF023922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative transposon protein TraM"
                     /protein_id="HEV9034974.1"
                     /translation="DSLAENIIAAVVDGAQTLVSGEEVTLRTAADAMLGGIRVPNGTA
                     LSGKAILSGERLLVTINATRIGSRVVPVALEVMDMDGMTGIRVQGSITRDASKESADQ
                     AMGALGITSMDAGVAGQVTAAGFQAAKTLLSRKIRLVRVGLPAGYKVWLKNTKSNR"
     gene            489..1313
                     /locus_tag="VGQ51_00090"
     CDS             489..1313
                     /locus_tag="VGQ51_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4138 domain-containing protein"
                     /protein_id="HEV9034975.1"
                     /translation="MKWRLMGVLLMGVVGVLKAQEPGFHLEGMESRTLPVTGRKMTNV
                     IFPVDIAAGVRVSRDVLVQKVKGVDNVIELKALRRDFAPTNLAVYAKDGRLYSFVLHY
                     VEDTSILDYRVVSDGSAVAPKVRLTGWPLDPETLQADARMLAARRSFLRQRVTSDGLG
                     LRLKGVYLRDSLLWLSMELQDRTNMGCTPGGLRIYTKDRKKVKRTATQELECAPAYSE
                     GFGVLSGPGREAAAVAFHPFVVSKGKRLVISFSDEDAGRQVILRVKGKVLMKTRKG"
     gene            1402..1617
                     /locus_tag="VGQ51_00095"
     CDS             1402..1617
                     /locus_tag="VGQ51_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034976.1"
                     /translation="MAILYCWLRQGREGPVRVSARELMPISKIGGLGPMYRSLRELHE
                     FGYIEYHPSYSPKGESWVYLPPLGSMG"
     gene            1949..2341
                     /locus_tag="VGQ51_00100"
     CDS             1949..2341
                     /locus_tag="VGQ51_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEV9034977.1"
                     /translation="MKKKGQPEPTRSELEILQVLWQHGPNTVRFVNDQLNEQKREVNY
                     ASTLKLMQIMAEKGLLRRDESQVKHIYSPASEEYKTKSLLLDRFVDTMFNGSVSSLML
                     HLLDNKKASQNELEAIRKLLNKTKNRES"
BASE COUNT          598 a          570 c          777 g          517 t
ORIGIN      
        1 cgattcgctg gcagaaaata ttatcgcggc agttgtggat ggcgcgcaga ccctcgtatc
       61 cggtgaagag gtgacgcttc gcacggcggc cgatgctatg ctcggtggaa tacgggtacc
      121 gaatggtacc gcgctatccg gtaaagccat cttgtcgggt gaacgcttgc tggttaccat
      181 taacgctaca cggataggca gtcgcgtggt ccccgttgca ctggaggtca tggatatgga
      241 tggaatgacg ggcattcgcg tccagggatc gatcaccagg gatgcgtcga aggaatcggc
      301 cgaccaggcc atgggcgcgc tgggtatcac ttctatggat gccggtgtgg ccggacaggt
      361 cacagccgcc ggcttccagg cggcgaagac cttgctgagc agaaagatca ggttggtgcg
      421 ggtggggctg ccggccggct acaaggtgtg gttgaagaat acaaagagta accgttaaaa
      481 atgtgtatat gaagtggaga ttgatgggag tgctgttgat gggtgtggta ggtgtactga
      541 aggcgcagga gcctggattc cacctggaag gaatggaaag caggaccttg cctgtgactg
      601 gtcgaaaaat gaccaatgtc attttcccgg tcgacattgc cgccggcgtt agagtcagcc
      661 gggacgtact ggtgcaaaag gtgaaagggg tggacaatgt gatcgaactg aaggcgctca
      721 gacgcgattt cgcgccgacg aacctggcag tttacgcaaa agacggccgg ttatattctt
      781 tcgtgttgca ttacgtcgaa gatacgagta tactcgacta tcgtgtggtg tcggatggga
      841 gtgcagtcgc accgaaggtg cggctcacgg gatggccgct ggaccctgaa accttgcagg
      901 cggatgcgag gatgttggca gcaagacgta gttttttgcg gcagcgggta accagcgatg
      961 gtctggggct gcgattgaag ggggtctatt tgcgcgacag ccttctttgg ctatcgatgg
     1021 aattgcagga ccggacgaat atgggatgca ccccgggcgg attgcgcatt tatacgaaag
     1081 atcggaaaaa ggtaaagcgg acggcgaccc aggagctgga gtgtgcgccg gcgtattcgg
     1141 agggattcgg tgtcttgtcg gggccgggac gggaggcggc agccgtggcc ttccatccat
     1201 tcgtggtcag caaaggcaaa cggctggtga tttctttttc cgatgaggat gccgggaggc
     1261 aggtaatatt gcgggtgaaa ggaaaggtgt tgatgaagac aaggaaaggt tgacatgaca
     1321 aaagcggttc ccgggataac ggattttatg gagcgggcga agagagaccc acgccttggg
     1381 cctctgcata ttagtttgta tgtggcgatc ctctattgct ggctgcgcca gggtagggaa
     1441 ggaccggtca gggtgagtgc aagagagctg atgccgatct cgaagatagg tggccttggg
     1501 ccgatgtata gatctctgcg ggaattacat gagttcggat atatagaata tcatccttca
     1561 tatagtccga aaggagagag ctgggtgtat ttaccgccgt tgggatcgat gggctaagat
     1621 agccgcgcgg ccatccagcc ggtattcata caccgtcgtt atttccccgt ccgacacgtc
     1681 gatcttccct ccgcgaaata ggtcaacgac ctttggaaat ggcccgtgcg atcatagagg
     1741 acgtgacaaa agacggagtc gcggagaccg aagtaagcgg aaaatcctcg cctggtcatc
     1801 atccattgtt cctgcgtcac cgccggatag gtctttctaa aatgccggaa ggccgaaatc
     1861 cgcgatccca tccgccgcga aatccgccgg ctaggaaata tttcctagaa ttgtttggat
     1921 gctaggaaaa aattcctaca ttaggagcat gaaaaagaaa gggcagccgg agccgacccg
     1981 ctccgaactc gagatactcc aggtattgtg gcaacacggc ccgaacaccg tgcgtttcgt
     2041 caacgatcag ctcaacgaac aaaaacgcga agtaaactac gccagcacgt tgaagctgat
     2101 gcagataatg gcggaaaaag gcttattgcg ccgcgacgaa agccaggtaa agcatatcta
     2161 tagccccgca tcggaggaat acaagaccaa gagtctcctg ttggatcgtt tcgtggacac
     2221 gatgttcaat ggctcggtca gcagcctcat gctgcatctg ctggacaata aaaaagcttc
     2281 ccaaaacgag ctcgaggcca tccgcaaatt gttaaataaa accaaaaaca gggagtcatg
     2341 aaacagctgt tcatcgatag cctattgacc gaccgggcag cacgggccat ttgctggacg
     2401 ctgatccact ccatctggca gggggtgctg gcagcggcgc tggcggccgt agtcatcgca
     2461 tg
//
LOCUS       DASYSV010000004         4618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_897040, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000004 DASYSV010000000
VERSION     DASYSV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897040"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VGQ51_00105"
     CDS             complement(<1..180)
                     /locus_tag="VGQ51_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034978.1"
                     /translation="MGRSVLSASSARSDMTANARRYAWIAALLLTALGGRAQYALHIL
                     PVDRDSVFILKKLGLD"
     gene            217..1353
                     /gene="hemW"
                     /locus_tag="VGQ51_00110"
     CDS             217..1353
                     /gene="hemW"
                     /locus_tag="VGQ51_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEV9034979.1"
                     /translation="MAGIYLHIPFCRQACLYCNFHFSTSMKGKNDFISALLKEMELRK
                     DYIGDEPVATVYFGGGTPSLLEPEELNGIMHRLRTLFPVGPGAEITLEANPDDISMER
                     LEAWKAAGINRLSIGVQSFFEADLRWMNRAHDAQQAIDCIGMAQSAGLTNLSIDLIYG
                     GPTLPDTHWRQNVERAIGLGVTHLSCYALTVEPRTALDTLIRRKQMTSVDPEDQARQF
                     LLLMEWMREAGYEHYEISNFALPGHRSRHNSSYWQGKPYLGLGPSAHSFNGISRQWNM
                     ANNAGYVAALSEGRGDLMAEREELTTIQQLNEYLMISLRTMEGINFRAIADRFGLPRA
                     EEVWQRAGRYMRSGKIGVSSEVLQLTQEGKLLADGIAADLFFEA"
     gene            complement(1488..2507)
                     /locus_tag="VGQ51_00115"
     CDS             complement(1488..2507)
                     /locus_tag="VGQ51_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV9034980.1"
                     /translation="MRFGVIGSGSWATALTKLLTDNGQTVNWWIRNTSAIRHIQTRRH
                     NPQYLTSVHFDTSLLSLSDSVQTVVAASDCLVMAVPSAYTAATLGQLDRTALQGKKVL
                     SAIKGILPEQNLLLNEFLVKEFNFPVADYFTIMGPCHAEEVAAKKLSYLTFSGQIETT
                     TRELASCFATQYLNTVINEDVYGVQYAAILKNIYALGAGIAHGLEYGDNFLSVLIANC
                     ADEMAGFLRKVGIRHIEVGVHEEDHRANYAASVYLGDLLVTCYSLYSRNRTFGNMIGK
                     GYSVKSAQLELNMVAEGYNASKCIYMINKEIGADMPIAGIIYRILWENVTAGIGFKQI
                     EPYLV"
     gene            complement(2515..3897)
                     /locus_tag="VGQ51_00120"
     CDS             complement(2515..3897)
                     /locus_tag="VGQ51_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9034981.1"
                     /translation="MNKSTRRILLIVGLLVVVIIVVAKSGAFGKNEGTRISTEKVVRR
                     NITEIVTASGKVYPEKEVKISPDVSGEVVELGVQQEGDSVHKGQMLARVYADILTNQR
                     DQAAAQVNQQQAMVSNVQEQLPGLKTAMETAKRALDRESQLRKDSVVSAAEYETAENT
                     YRTAQANYMAALQTVRGNEAGASSAKYNLDVAAKNLSRTTVVSPIEGVVSLLSIKKGE
                     RVVGNSMMAGTEMMRVADMSRIETIVDVGENDIPKVQLGDSAIISVDAYNNRKFRGIV
                     TQIASSVSTASGTSTTTVSTNDVTNYKVHIRLSPDSYKDLFDPAHPRHFPFRPGMSAS
                     ADIQTRTHVNVLAVPINSVAVREKNSDNAVLANTDDNSGGDNKADVNNAASGDLDEVV
                     FVVQKGDTVRKAKVRTDIQDINYIEVVDGLKEGDEVVTGPYSVISKSMKSGQRIKVVP
                     KDKLFTEKKN"
     gene            complement(3917..>4618)
                     /locus_tag="VGQ51_00125"
     CDS             complement(3917..>4618)
                     /locus_tag="VGQ51_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9034982.1"
                     /translation="YMGMDAAAPFDITTPPVEDIPVENIADLQPDIVYAMALVSQPAQ
                     RMDSLLIAAIRRDVRAISAGRFPTVTMGGGLSSTYFKYSNIPADKYFTQINNNFAQSV
                     GININIPILNGGALRTNYEKSKLNLQNGLLARDQDNLTLKQNIYMAYTAAMTALQKFE
                     ANKISVAASEKSYLFAKKRYDVGMLNTIDLLTNQNNFFSARINLLTSQFDYVFKMKIL
                     EYYKGLGVKLTKGGN"
BASE COUNT          979 a         1201 c         1290 g         1148 t
ORIGIN      
        1 gtcgagccct aattttttaa ggataaaaac agaatccctg tcgacgggaa ggatatgaag
       61 agcgtattgc gcccggccgc caagggcagt cagcagtagg gccgctatcc acgcatatcg
      121 cctcgcgttt gcggtcatgt ccgatcgggc cgagcttgcg gacaggaccg atcggcccat
      181 gcttgcggac aatacagatc ggcgtaattt tgcccgatgg caggcattta tctccatatt
      241 cctttttgca ggcaggcctg tctttactgc aatttccatt tttccacgtc catgaaggga
      301 aaaaacgatt ttatctctgc tttattgaaa gaaatggagc tcaggaagga ttatatcgga
      361 gacgagccgg tggccacggt atatttcggc ggcggtactc cttccctgct ggagccggag
      421 gagctcaacg gcatcatgca ccgcctcagg actctttttc cggtggggcc gggagcagag
      481 atcaccctcg aggcgaatcc cgacgacatc agcatggaaa gactggaagc ctggaaggcc
      541 gccggcatca atcgtctcag tatcggtgtt caatcttttt tcgaagccga tctgcggtgg
      601 atgaaccggg cccatgacgc gcagcaggcc atcgattgca tcgggatggc gcagtccgcg
      661 ggattgacga atctttcgat cgatctgatc tatggcggtc ctaccctgcc cgacacccac
      721 tggcggcaga atgtggaacg ggccatcggt ctgggagtta cccatctttc ctgctatgcg
      781 ctgacggtag aaccccgcac ggccctcgat accctgatcc ggcggaagca aatgacgagc
      841 gtcgatccgg aagatcaggc aagacagttc ctgctgctca tggagtggat gcgcgaggca
      901 ggatacgagc attatgagat ctccaatttc gcgttgcccg ggcatcgaag ccggcacaac
      961 tcttcctatt ggcagggtaa gccatatctg gggttagggc cttcggccca ttccttcaac
     1021 ggcatatcgc gtcagtggaa tatggctaat aatgcgggat atgtggcggc gctgtctgag
     1081 gggcggggtg acctgatggc agaaagagaa gagctgacga caatacagca attgaatgaa
     1141 tacctcatga tctccctgcg aacgatggaa ggaattaact tccgggcaat tgcagatcgt
     1201 ttcggtttgc cgcgcgcgga agaagtgtgg cagcgggcag ggcgatatat gcgatcggga
     1261 aaaatcggag tctcttcaga ggtattacaa ctgacacagg aaggaaaatt gctggcggac
     1321 ggtatcgcgg ctgacctgtt cttcgaggcc tgatcttatt gacggtgatg cctgcgcgat
     1381 ctgagatatt ctatgccctg ggcggccgac aggatcagca ccattgcgag aatggccatc
     1441 gcaccaaaac cctggaagga gagaggcata ataatcggtt ttattcctta tacaagatac
     1501 ggctcgatct gtttaaatcc gataccggcg gtcacatttt cccagaggat gcggtagatg
     1561 atcccggcga tgggcatatc ggcgccgatc tctttgttaa tcatgtagat acatttgctg
     1621 gcgttataac cttcggcgac catgtttagc tccagctggg ccgatttgac cgaatacccc
     1681 ttgccgatca tatttccgaa ggtgcggttg cggctataga gcgaatagca agtgacgagc
     1741 aggtccccga gataaacgga tgcagcatag ttggcccgat gatcttcctc atgcactcct
     1801 acttcgatat gccggatgcc caccttccgc agaaagcccg ccatctcatc ggcgcagttg
     1861 gcgatcagga cactgagaaa attatcgccg tattcgagac cgtgggctat tcccgcgcca
     1921 agcgcataga tattcttcag gatagcggcg tattgcacgc catagacatc ctcattgatc
     1981 acggtattca ggtactgtgt cgcgaagcag gatgcgagtt cgcgcgtagt cgtctctatc
     2041 tgtccggaaa aagtgagata ggacagcttt tttgcagcga cctcctccgc atggcagggc
     2101 cccatgatcg taaaataatc ggccaccggg aaattgaatt ccttcacgag aaattcgttc
     2161 agcaggagat tttgttcggg gaggatgcct ttgatggccg acaggacctt ttttccctgc
     2221 agcgcggtcc tgtccagctg gccgagggtc gccgcggtat aggccgaagg aaccgccatg
     2281 accaggcagt cggatgcggc tacgacggtt tgcacggaat cgctcagcga caataaggac
     2341 gtgtcgaaat gtacggaggt caggtattgc gggttgtgac gcctggtctg tatatgccgg
     2401 atggccgagg tattcctgat ccaccagtta acggtttgcc cgttgtccgt caaaagcttc
     2461 gtcaacgccg tagcccagct accgctgcct atcacaccaa aacgcatacc cctcctagtt
     2521 tttcttttct gtaaatagct tgtctttcgg gacaacttta atgcgctgtc cgcttttcat
     2581 agatttgctg atgacgctgt agggaccggt aacaacctcg tcgccttcct ttagcccgtc
     2641 cacgacctcg atatagttga tgtcctgaat gtccgtacgg actttcgcct tgcgaacggt
     2701 gtcgcctttc tgcactacga acacgacctc atccagatcg ccgctggcgg cattgttgac
     2761 atcggccttg ttgtcgccgc cggaattgtc atcggtgttg gccaataccg cattgtcgga
     2821 attcttttcc cggacggcaa cggaattgat ggggacggcc agtacattca catgtgtcct
     2881 ggtctggata tccgcgctgg cgctcatacc gggccggaag gggaaatgcc gggggtgtgc
     2941 ggggtcgaag aggtctttat acgaatccgg cgaaagacgg atatggacct tgtaattggt
     3001 gacatcgttg gtcgacacgg tggtggtaga tgtgccggac gcggtgctga cactgctggc
     3061 gatctgggtg acgatgccgc ggaatttgcg gttgttgtac gcatccactg aaatgattgc
     3121 ggaatcgccc agctgtactt tgggaatatc gttctcaccg acgtcgacga tggtctcgat
     3181 cctgctcata tcggccacgc gcatcatctc ggtgcccgcc atcatggaat tgcctacgac
     3241 gcgttcacct ttcttgatgc tcagcagcga aacgacgcct tcgatcgggg aaacgacggt
     3301 agtacggctg aggttcttgg ctgcaacatc gagattgtat ttggcgctgc tggctcctgc
     3361 ttcattgcca cgtacggtct gcaaggcggc catataattg gcctgtgccg tcctgtaggt
     3421 attttctgcc gtctcatatt cggcggcgga gactacacta tccttgcgca gctgtgattc
     3481 acgatcgagc gccctttttg ccgtctccat ggccgtcttc aacccgggca gttgttcctg
     3541 tacgttggag accatcgcct gttgctgatt gacctgtgcg gcggcctgat cgcgctggtt
     3601 ggtgaggata tcggcgtata cccgcgccag catttgtcct ttatgcacgc tgtcgccttc
     3661 ctgctgtacg ccgagctcta ccacttcacc cgaaacgtcg ggactgatct tgacctcttt
     3721 ttcgggatat accttaccgc tggcggtgac gatctccgtg atattccggc ggacgacttt
     3781 ctccgtcgaa atccgggtgc cctcgttctt tccgaaagcg ccggacttgg cgaccacgat
     3841 gataaccacg accaggaggc cgactattaa aaggatgcga cgggttgact tattcattgt
     3901 cggtattatt ctttggttaa tttccgcctt tggttaattt caccccgagc cctttatagt
     3961 attcgaggat cttcatcttg aatacataat cgaattgcga agtgagtaag ttaatgcggg
     4021 cgctaaaaaa attgttctgg ttggtcagca ggtcgatggt attgagcatg ccgacatcgt
     4081 atcttttctt tgcaaacagg tagcttttct cagaagccgc aacgctgatc ttgttcgcct
     4141 cgaacttctg gagcgccgtc atcgccgcgg tatacgccat gtaaatattt tgcttgagcg
     4201 tcaggttgtc ctggtcacgg gccagcaggc cgttttggag gttcagcttg ctcttctcat
     4261 aattcgtccg cagggctccc ccgtttagaa tgggtatgtt gatattgatc cccacggatt
     4321 gcgcaaaatt attgttgatc tgggtgaaat acttatccgc cgggatgttg ctgtatttga
     4381 aataagtgga gctcagtcct ccgcccatgg ttaccgtagg aaaacgccct gccgatatgg
     4441 cgcggacatc tctgcgaatg gcggcgatca gtaaggaatc catccgttgc gccggctggc
     4501 tgaccagcgc catcgcgtat acgatatcag gctgaagatc ggcaatattt tcgacgggga
     4561 tatcttcgac gggcggggtg gtaatgtcga atggcgcggc ggcatccatc cccatata
//
LOCUS       DASYSV010000005         8294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1793570, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000005 DASYSV010000000
VERSION     DASYSV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793570"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VGQ51_00130"
     CDS             <1..1007
                     /locus_tag="VGQ51_00130"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEV9034983.1"
                     /translation="LLAMAFSGLTASAQRTDTVYLYNGQVLIGEVQSGSLGTLYIDDI
                     DMKIVKVKYYKIRRMNISETFKITTIGKQTYYGVLMAAQKPGWGIVQDEAGFVMPLAL
                     VDILDLVPLNKEFFKRLVGNLSAGFSYTKSSNIGQLNLSANVGFATKKWEFQLQTSEI
                     GSIDSGKFSRDNESIQLFGAYTLSNNWFLAGALQYQRNLELSIARRYLELMGAGNKLV
                     IRDTWQLYAITGLSVTQEKSTEGVASPTALEIPLMFRFNFYKFQHPDIQISTSWTGYF
                     SLTEKGRFRYDGNTSFSWQLIRYFYLTINPYSSFDTKPPSGTGAEFDFGIAMSLTYKF
                     "
     gene            1158..1793
                     /locus_tag="VGQ51_00135"
     CDS             1158..1793
                     /locus_tag="VGQ51_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9034984.1"
                     /translation="MIGILVYEDNDSLRESLANLLMLSKDLLLLGSFPRADDISAHIA
                     RYNPEVILMDIEMPGLNGIEAVRQVRTLDKKVNIIILTVFDDNKHVLDAICAGASGYL
                     LKKHLSDHLPEAIRQVMDGEPPMSRGIARMIIAGMQQTATGPTPNDYKLSAREKEILS
                     HLSKGEGLKAMASELNISLDTVRTHIKNIYEKLQVHTQTEAVSKAINEKLV"
     gene            complement(1842..2039)
                     /locus_tag="VGQ51_00140"
     CDS             complement(1842..2039)
                     /locus_tag="VGQ51_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034985.1"
                     /translation="MNAIVGGVDIGFTVSSGVWEYNPATDVWGQVQNMPHTQGDADGG
                     FVLGNSLFVAGLNAVALNWTR"
     gene            complement(2096..3133)
                     /locus_tag="VGQ51_00145"
     CDS             complement(2096..3133)
                     /locus_tag="VGQ51_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034986.1"
                     /translation="MHNKFSVGISLLLCGVLVSCQKETTPEAGEAGASSNKKTTTGLV
                     QGPPTIVWWANGIAVPYVNQFGTPAELSQDAAGFSINGKGYLCGGGQFYEGTGGVYSS
                     DLWEYDPSGPIWTKKAPIPAAVNNAVAFVIGGKAYLVLGNTTWQYDPAVDQWSQKASL
                     PTVNRQFGSGFAINGKGYVGLGYDDVTTAFQNDWWEYDPSTEHWTAKKKFPGTKVYAA
                     PAFSVGGKGYIVSGRHGAQSFPTAVWQYNPATDNWTQKADFPGEGRSSAVGASGTVDG
                     VDVGFVAGGVPGTPLIPINKDAWEYFPASDTWGRLPDMPGGGRTDAGWFVINRSLFIA
                     NISVVVLNWSN"
     gene            complement(3151..4194)
                     /locus_tag="VGQ51_00150"
     CDS             complement(3151..4194)
                     /locus_tag="VGQ51_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034987.1"
                     /translation="MYGHYLILLSLVIVSCRKENTPASADDASSSNKKATTGLVQGPP
                     PIVWWSDGPTIPYADDIPGDVPRNNEYGQGFSINGKGYVCGSLLTTGWNTGDYIGDLW
                     EFDPATESWTEKSPCPLAGGQLIEAANFVIGDNAYIVIGNATYQYNQPTDTWTQKAYI
                     FSVPRALATAFTINDKGYIGLGYDQSSSQVSEQNDWWEYDPVADRWTQKAAFPGAKRE
                     GAAGFAVNGKGYVIGGSHFANGHGNWGSKVWQYDPVTDAWSQKSDFPGAGRYEAVAAT
                     GTIGGVELGLLVGGGNANTLFTDAWEYNSATDNWGQFANMPGGGRYVPAGFVVGHSLC
                     ITNKTMVSLNWSR"
     gene            complement(4255..4746)
                     /locus_tag="VGQ51_00155"
     CDS             complement(4255..4746)
                     /locus_tag="VGQ51_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034988.1"
                     /translation="MEYDRQKRIVRGWIVFFIIALVLSGATAFALESELNWLLAHWPG
                     RADSALHYWVLKVYDALQDMNARYPFLAYGYDWLAFAHLVIAVVFIGVYRDPVRNKWI
                     LGFGMIACVMVFPLAWIAGAVRGIPVYWRLIDCSFGVIGIVPLFICYRKVVWMEKMGA
                     PLP"
     gene            complement(4823..5101)
                     /locus_tag="VGQ51_00160"
     CDS             complement(4823..5101)
                     /locus_tag="VGQ51_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034989.1"
                     /translation="MDTENEYEDMRDQRQALVLWLRFVAVGLVVILLVRIFGSQVSWD
                     LTGKLTVNNIAGLAERSGDAGRWLVDHIYFLLPLVLLFFRRRMRDQNV"
     gene            complement(5091..5330)
                     /locus_tag="VGQ51_00165"
     CDS             complement(5091..5330)
                     /locus_tag="VGQ51_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9034990.1"
                     /translation="MLAKDQTRMIDHALEKLEPEDRLVPTLYYLDEQSIGEVAAITGI
                     PGENIKMRLHRARKRMYGILSRLVNAETKTIENGH"
     gene            complement(5760..6047)
                     /locus_tag="VGQ51_00170"
     CDS             complement(5760..6047)
                     /locus_tag="VGQ51_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034991.1"
                     /translation="MGNRDSRYDLIKPMLSEGKIVSFTDIFKYIPKTIVANDLGKKVD
                     RFTVMMNQVEKFTLEELFKMAALCRLTKDQMFGLLLQEYVKKHATEVLAPE"
     gene            6308..>8294
                     /locus_tag="VGQ51_00175"
     CDS             6308..>8294
                     /locus_tag="VGQ51_00175"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV9034992.1"
                     /translation="MYFTKTYFSFKYGTFSTEQLIRTGVEAGATSLTLTNINSTCDAW
                     DFVRYCGREGIKPILGVEIRNDNTLLYTLIAANNNGFRQINEFLSDHLLEKKPFPPAD
                     ALQPPFSKITDGFIIYPLGGKPPEQLLDNERIGILPREAHRLIRMPLASCPDKWIIRQ
                     PVTFQDKRYYNLHRLLRAMAANTLLTKLKPEHLAGEDEYFIPPVEMMTAFPQYPFIIT
                     NTARLIDACSIEMNFGEHKNKKMYGASREDDRVLLEKLARDGFRFRYGANKKIKQRLD
                     KELRIIDQMSFTAYFLITWDMIRYAQSRGFYHVGRGSGANSLVAYCLRITDVDPIELD
                     LYFERFLNPHRTSPPDFDIDFSYRDRDEVIDYMMKRYGRKYTTLLGMYPTFQYNAIIR
                     ELGKVFGLPKAEIDALAGGSQNSNDKIHMAILRYGRLLQNFPSGLSIHPGGILISEEP
                     IASWCAVHMPPKGFQTSQMDMFVAEDIGLYKLDVLSQRGLGHIKECTELIKKNHRTDI
                     DIHEIDKFKKDPAVKEQLRAGNTIGCFYIESPAMRQLIKKLHCDNYEGLVAASSIIRP
                     GVGRSGMMQQYIERHNNPDKFEYAHPRLKDLLGSTYGVMIYQEQVIQVAHEWAGLDMA
                     DADLLRRATSSKYRGKSQLPMLKEKFFAGCAAYDYPTP"
BASE COUNT         2050 a         2527 c         1876 g         1841 t
ORIGIN      
        1 atcttttggc aatggccttt tcaggcctca ccgcatctgc gcagcggacc gataccgtct
       61 acctctacaa tggccaggta ctcatcgggg aagtgcagag cggttcattg ggtaccctat
      121 atatagatga catcgacatg aagatcgtca aggtcaaata ttacaagatc aggagaatga
      181 acatcagcga aaccttcaag atcacgacga tcggaaaaca gacctattat ggggtcttga
      241 tggcggccca aaaacccggt tggggaatcg tacaggatga ggcagggttc gtcatgccgt
      301 tggcactggt cgacatcctt gacctggtac ccctgaacaa agaattcttc aaacgattgg
      361 tcggcaacct atccgccggt tttagctata ccaaaagcag caatatcggt cagctcaacc
      421 tcagcgcgaa cgtcggtttc gccaccaaaa aatgggaatt ccagctgcag acatcggaga
      481 tagggtcgat cgattccggc aaattttcgc gggataacga atccattcag ctattcggcg
      541 cctatactct cagcaacaat tggttcctgg cgggggcttt gcagtatcag cgcaatctgg
      601 aactgtccat cgcacggcga tatctcgaac tgatgggagc cggaaacaaa ctcgtcatcc
      661 gcgatacctg gcagctatat gccatcaccg gtctcagtgt gacgcaggaa aagagtacgg
      721 aaggcgtggc cagccctacg gcgctggaga tcccgctgat gttccggttc aatttttaca
      781 aattccagca tccggatatc cagatctcga cgtcctggac ggggtatttc agtctcacgg
      841 aaaagggccg tttccggtat gatggcaata ccagcttttc ctggcagctg atccggtatt
      901 tctacctgac gatcaatcct tacagcagtt ttgacaccaa accgccctcg ggaacgggtg
      961 ccgaattcga tttcgggatt gcgatgagcc tgacttataa attctgaagg agcccgcacg
     1021 gatctccgat tcccggcgtt gccgtttcct ggtgcgggtc agcaccggtc acctgtttgc
     1081 ggtatgccca cccctgaaaa attcggtatc ttgccgaagg tctcaacacc tcatccgaga
     1141 cttcaacaaa tcatcagatg atcggcatcc tggtatacga ggacaatgac agcctgcgcg
     1201 aaagcctggc caacctgctc atgctctcga aagacctcct cctgcttggc tccttccccc
     1261 gcgccgacga catctccgct cacatcgccc ggtacaaccc cgaggtcatc cttatggata
     1321 tcgaaatgcc gggtctcaac ggcatcgagg cggtgagaca ggttcgcacg ctcgacaaaa
     1381 aggtcaacat catcatcctc accgttttcg atgacaacaa acacgtcctc gacgccatct
     1441 gcgccggcgc ttcaggctac ctgctcaaaa agcacctctc cgatcatctc cccgaagcca
     1501 tccgccaggt gatggatggc gagccgccca tgtcccgcgg catcgcccgc atgatcatcg
     1561 ccggtatgca gcaaaccgcg accggaccca cacccaacga ttacaaactg tccgcccgcg
     1621 aaaaagagat actctcgcat ctctccaaag gcgaaggact caaggcaatg gcctccgagc
     1681 tcaatatcag cctcgatacc gtccgcacac atatcaaaaa catctacgaa aaattacagg
     1741 tccacaccca gaccgaagcc gtctccaagg ccatcaatga aaaattggtg tgaccggccc
     1801 cgccgaatca caccaacgct ttccgggtcg gaaccaaaat gctatctcgt ccagttgagc
     1861 gcgactgcat tcaatccggc aacgaacaaa ctattcccca gcacgaatcc cccatccgca
     1921 tccccctggg tgtgcggcat attctgcact tgcccccaga catctgttgc cgggttatat
     1981 tcccaaacac ccgagctcac ggtaaaaccg atatccacgc cgcccacgat cgcattcacg
     2041 cggaatgacc ccgcgggaca aaaaagaatt acgcgtattc ataaaatttg attgatcagt
     2101 tcgaccagtt tagcaccacg acagatatat tggcgataaa aagacttcga ttgatgacaa
     2161 accaacctgc atcggtccgg cccccgcccg gcatatccgg cagtcgtccc caggtatcgc
     2221 tggcaggaaa atactcccag gcatccttgt tgatagggat gaggggcgtg cccggtaccc
     2281 cgcctgctac aaaaccgaca tcgacgccgt cgacggtgcc gctcgcgccg accgcagacg
     2341 agcggccttc accggggaaa tctgcttttt gcgtccagtt atccgttgca ggattgtact
     2401 gccataccgc cgtcggaaaa ctttgagcgc catgtcttcc gctgacgata tagcctttcc
     2461 cgccgacaga aaaagcaggt gccgcatata ccttcgtacc gggaaatttt ttctttgccg
     2521 tccaatgctc cgttgaggga tcgtattccc accagtcgtt ttgaaaggct gtggtcacat
     2581 cgtcataacc caagcccaca tatcccttgc cgttgatcgc aaagccgctg ccaaattgcc
     2641 ggttgacggt cgggagagac gctttctgcg accactggtc aacggccgga tcgtattgcc
     2701 aggtcgtatt gccgaggacg aggtatgcct ttcccccgat aacgaacgcg actgcattgt
     2761 tgaccgctgc gggaatgggc gcctttttcg tccagatcgg gcccgatgga tcatattccc
     2821 acagatcgct gctgtataca ccgccggttc cctcatagaa ttgcccgccg ccgcatagat
     2881 atcccttgcc gttgatggaa aaacccgccg cgtcctgact aagctcggcc ggggtgccga
     2941 attgattcac atacggaaca gcgatcccgt tcgcccacca aacgatcgtc ggcggcccct
     3001 gcaccagccc ggtagtcgtc ttcttattcg atgaagcacc agcttcgccg gcctcgggag
     3061 tagtctcctt ctgacaagaa acaagaacac cgcaaagcaa cagcgatatt ccaacggaaa
     3121 atttattatg catatacata gctttgattt ttatcgtgac cagttcaggg agaccatggt
     3181 cttattggtt atacacaggc tatgcccgac tacgaaacca gccgggacat aacgaccccc
     3241 tcccggcata ttcgcaaact gtccccagtt gtcggtagca gagttatatt cccaggcatc
     3301 ggtaaaaagc gtgttggcat tgcctcctcc caccaataat cccaactcga ctccaccgat
     3361 cgttccggtt gccgcaacag cctcgtatcg cccggctccc ggaaaatcgg atttttgcga
     3421 ccaggcatcg gttacaggat cgtactgcca tactttactc ccccagttgc catgaccatt
     3481 ggcaaaatga ctgcccccga tgacataacc cttgccattc acagcaaagc ccgccgcacc
     3541 ttctctcttt gcaccgggga atgctgcttt ctgcgtccaa cggtctgcaa ccggatcgta
     3601 ttcccaccag tcgttttgct cggatacctg gctgctgctc tggtcatagc cgagcccgat
     3661 atatcctttg tcgttgatgg tgaaggccgt ggcaagcgcg cgggggacag aaaagatata
     3721 cgccttctgt gtccaggtgt cggtaggttg attgtattga taggtggcat tgccgatgac
     3781 gatataggca ttatcgccga tgacgaaatt tgcagcctcg ataagctggc cgccggcaag
     3841 ggggcaggga ctcttttccg tccagctctc ggtagcggga tcgaattccc atagatcgcc
     3901 gatgtaatca cccgtattcc agccggtggt cagcaaactt ccgcagacat aacccttgcc
     3961 gttgatgctg aatccctgcc catattcgtt attcctgggc acatcaccgg ggatatcgtc
     4021 cgcatacggg atcgtaggtc cgtccgacca ccagacgata ggcggcggac cctgtaccag
     4081 acccgtggta gctttcttgt tcgatgaaga tgcgtcgtcg gctgacgcgg gtgtattctc
     4141 tttccggcag gaaacgatga ccagggagag aagtataaga tagtgcccgt acatgatcgg
     4201 agtgtttaat aggcacaaat ttccaaacaa ctccggccat agtcaataca ccaatcaggg
     4261 tagtggcgct cccatttttt ccatccatac cactttccgg taacagataa acagcggcac
     4321 gatcccgatc acaccaaacg aacaatcgat cagtcgccag tagaccggta tgcccctcac
     4381 cgcgcccgcg atccacgcca acggaaaaac catgacacaa gcgatcatcc cgaaaccgag
     4441 gatccattta ttgcgcaccg ggtcccggta aaccccaatg aacacgaccg cgatcaccaa
     4501 atgcgcaaac gccagccaat catagccata ggccaggaaa ggataccgcg cattcatgtc
     4561 ctgcaaggca tcatagactt tcaataccca gtaatgaagc gcactgtccg cacgaccagg
     4621 ccagtgagcc aatagccaat tcaattcgct ttccagcgca aaagccgtcg ccccgcttag
     4681 cacgagggcg atgataaaga acacgatcca cccgcgaacg atcctttttt gccgatcata
     4741 ctccatatat aaaagtgctt tgcactgcaa agtaaacata attatttcat cataccaaat
     4801 ccttttccgc ccgttttgtg aactaaacgt tctgatctct cattctccgc ctgaagaaca
     4861 gcagcaccaa cggcagcaaa aagtaaatat ggtccaccag ccatctgccg gcatcacccg
     4921 acctttcggc caaaccggcg atattgttga ccgttagctt ccccgtaaga tcccagctca
     4981 cctgggaacc gaagatcctc accaaaagga tcacaaccag acccaccgcg acgaagcgta
     5041 gccataagac aagggcctga cgctgatccc gcatgtcttc atattcattt tcagtgtcca
     5101 ttttcaatag tttttgtttc ggcatttacc agcctgctca ggataccgta catcctcttt
     5161 cgggcccggt gcagccgcat cttgatattt tctcccggaa tgcccgtaat cgccgcgacc
     5221 tcaccgatcg attgctcgtc gagataatac aacgtgggca caagtctgtc ttccggttcc
     5281 agtttttcca acgcatgatc gatcatacgc gtctgatcct tcgccagcat tcggcggtat
     5341 cccgactcga tgctccccta gagacaccgg cgatgatgat gccggtcaaa tcttgtgttg
     5401 ccatgttatt gaaatttaag ttattaaaat cttatgtcct attagatgcc gatacatttc
     5461 tcccggtaac cgcctccgcg gaatcttcta ccggggcccg attcgaccac cccacccgct
     5521 ccgatccacc cccattccct tcgcttcatc cgctccgatc cacccgcatc ccctccccat
     5581 ccgcttcgat ccacccatac ccttcacccc atccgctccg atccacccca aaaaaatata
     5641 ggccgcccct ttgggggcgg cctatgcgga agaggcctgg ttccggatcg attcgtaaaa
     5701 tggataaggg cccgctacgg gcaatggtaa tggaacttca aactagaact cgaaaaactt
     5761 tactccggtg ctaacacttc cgtcgcatgc ttttttacat attcctgcaa taacaacccg
     5821 aacatctgat ctttggtcag ccggcacaat gcggccatct tgaacaactc ttccagggtg
     5881 aatttctcga cctgattcat catcacggtg aaccggtcca ccttcttgcc cagatcgttt
     5941 gcaacgatcg tcttggggat gtatttgaag atatcggtaa aggacactat tttaccttct
     6001 gacaacatgg gcttgataag gtcatagcga gaatctctgt ttcccataac acaaaaaaaa
     6061 gttttttcga attataaatt ggttgtgcaa agacatttat tttcacccgc ctttttttgc
     6121 taccctgcgc gtcatttttt cgatccatct tctacttttt cttactccca tttttacagt
     6181 tatcgctaca atccgctgca gcaaaggctc acaaggcatt ttgctgaaac aaaattacta
     6241 ataattttag tatttagata ctaaaattat taggtagttc ataactttgt tcccacaaga
     6301 ccttgcgatg tatttcacca aaacctattt tagtttcaaa tacgggacct tctccacgga
     6361 acagttgatc aggaccggcg tcgaagccgg cgccacctca ctcaccctca ccaatatcaa
     6421 ttccacctgt gacgcctggg acttcgtccg gtattgcggc cgggagggga tcaagccgat
     6481 cctgggcgtg gagatccgga acgacaacac gttgctttat accctgatag cggccaataa
     6541 taatgggttc cgccaaatca acgaattcct gtccgatcac ctgctggaaa aaaaaccctt
     6601 cccgccggcc gatgccctgc agccaccttt cagcaagatc acggacggat tcattatcta
     6661 tcccctcggc ggcaagccgc ccgaacaatt gctggataac gagcgcatcg gcatcctccc
     6721 ccgcgaggcc caccggctca tccgaatgcc actggcatcc tgcccggata aatggatcat
     6781 ccgccagcct gtcacctttc aggacaaacg ctattacaac ctgcaccggc tcctgcgggc
     6841 catggcagcc aacacactgc tcaccaagct caaacccgaa cacctcgccg gcgaagacga
     6901 atatttcatc cccccggtgg aaatgatgac cgccttcccg caatatccgt ttatcattac
     6961 caataccgca cgtctcatcg acgcctgctc catcgaaatg aatttcggag aacataagaa
     7021 taaaaaaatg tacggcgcca gccgcgaaga tgaccgcgtg ctgctggaaa agctggccag
     7081 ggacggcttc cgctttcgct atggcgccaa taagaaaata aaacaacggc tggataaaga
     7141 gctccgtatc atcgatcaga tgagcttcac cgcctatttc ctcatcacct gggacatgat
     7201 ccgctatgca cagagccgcg ggttctatca tgtcggacgc ggcagcggcg ccaattccct
     7261 cgtggcctac tgtctccgca tcaccgatgt tgaccccatc gaactggacc tctatttcga
     7321 acgctttctc aacccccacc ggaccagccc gcccgatttc gatatcgatt tctcctaccg
     7381 cgaccgtgac gaggtcatcg attatatgat gaaacgctac ggcaggaaat atacgaccct
     7441 gctaggcatg taccccactt tccagtacaa cgccatcata cgcgagctgg gtaaggtatt
     7501 cggcctcccc aaggctgaga tcgatgcgtt ggccggcggt agccaaaact ccaacgacaa
     7561 aatacacatg gccatcctcc gctacggccg cctcctccaa aatttcccca gcggtctcag
     7621 catccacccc ggcggcatcc tcatttccga agaaccgatc gcctcctggt gcgccgtaca
     7681 catgccccca aaaggcttcc agacttccca gatggatatg ttcgtcgctg aagacatcgg
     7741 tctctacaag ctcgacgtcc tcagccaacg cggtctcgga cacatcaaag aatgcacgga
     7801 gctcatcaaa aaaaaccacc gcaccgatat cgatatacac gagatcgaca aattcaaaaa
     7861 agatccggcc gtcaaagaac agctccgtgc cggcaatacc atcggctgtt tctatatcga
     7921 atccccggcc atgcgacagc tgataaaaaa actccattgc gataactatg agggcctcgt
     7981 ggcggctagt tccatcatcc gtcccggggt aggccgcagc ggcatgatgc aacaatatat
     8041 cgagcggcac aacaatcccg ataaattcga atacgcacac cccaggctca aagacctgct
     8101 gggctccacg tatggtgtca tgatctatca ggagcaggtc atccaggtgg cccatgaatg
     8161 ggccgggctc gacatggccg atgcagatct gctccgccgg gccaccagct ccaaataccg
     8221 cgggaaaagc cagctgccta tgctgaagga aaagttcttc gccggctgcg ccgcgtacga
     8281 ttatcccaca ccgg
//
LOCUS       DASYSV010000006         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_897265, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000006 DASYSV010000000
VERSION     DASYSV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897265"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VGQ51_00180"
     CDS             <1..593
                     /locus_tag="VGQ51_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV9034993.1"
                     /translation="INYSWPGNVRELKNIAEQVSVLSEEKHVSASALRRFLPENTGTR
                     LPMVMPSNGIGGNGSHEFANEREILYKLFFDLRKDVTELKKILKEIVQNPSLAQATHA
                     AYINGLNELKPAELYQPPALQTVQPMVLHNNINGHEEIHHHEEVEESLNIMDKEKELI
                     VKALKKHKGKRKDAASDLGISERTLYRKLKEYDINE"
     gene            586..1131
                     /locus_tag="VGQ51_00185"
     CDS             586..1131
                     /locus_tag="VGQ51_00185"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HEV9034994.1"
                     /translation="MNKVKITGAFIFAVVAIVLASGLSSCHVYSFKDVSIPPEVKSIH
                     LSYIENRAPLVNPQLAPQLNDALRQKINNQASRLSQVQTSDADYDVSGWVSGYSYSTS
                     GISNQQASSNRLTVTVHIVFKNHLDPTGKKVAPADFETDVSRNFDFSASASITDAEQQ
                     LMPTIISNLTDEIFNKLFSNW"
     gene            1143..2342
                     /locus_tag="VGQ51_00190"
     CDS             1143..2342
                     /locus_tag="VGQ51_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9034995.1"
                     /translation="MNQPLQSIVQHLFQVTSLDDVSRQRLESFVVEYPSFGIGHYLLS
                     HKLRTEDPDRYRDTTQRTCLYFSNPFWLEWQLTNSPDSDGPAHETVAPGHSETSVLPV
                     EEPHTIESQQIDSQEVEIPGDEVQSAQPQSAEPQFNEPQSVESLTVDIPGETIEEATV
                     AEPPAAPTETIVEEPADSNEVSAADQLLLSIEEARGLRDSLQKISEDFTVDAVPEQQP
                     IQDQEVPFVLDEPQEAPAPLPENPEPIAAVNAAEPQEAPTEPPPAPTPEPVPAPPTPE
                     PIFEPYHTIDYFASQGIRLKLDENPADALGKQLKSFTDWLKIMRRLPQKDRDSSVPDR
                     AAEQAVQNFAAHSIQSKDIVTETMAEVLIKQGMRDRARVIYEKLSLLNPDKKAYFAAK
                     IEQINIP"
     gene            2344..2628
                     /gene="secG"
                     /locus_tag="VGQ51_00195"
     CDS             2344..2628
                     /gene="secG"
                     /locus_tag="VGQ51_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV9034996.1"
                     /translation="MIFFVILIVLACVILGLIILVQNPKGGGLAGNVGGFSNQLMGVK
                     QTTDVLEKGTWVLAVVVALLSLFSTVFVPKATNGRDIQKVNVPMQQQSRP"
BASE COUNT          671 a          755 c          665 g          539 t
ORIGIN      
        1 taattaatta ttcctggccg ggcaatgttc gggaattgaa gaatattgca gaacaggtgt
       61 ccgtcctttc ggaggaaaag catgtttcgg cgtcggcgct tcggcgcttc ctcccggaaa
      121 acaccgggac cagattgccg atggtaatgc cctccaacgg gatcggcggt aacggctcgc
      181 atgagttcgc caacgaacgc gagatcctgt acaagctgtt cttcgatctg cgaaaggacg
      241 tgaccgagct gaaaaagata ttgaaggaga tcgttcagaa cccgtcgctg gcccaggcca
      301 cgcatgcggc gtatatcaac gggctcaacg agctgaagcc ggccgagctg tatcagcctc
      361 ccgcgctcca gaccgtacaa cccatggtgc tgcataacaa catcaacggt catgaagaga
      421 tacatcacca tgaagaagtg gaagaatcgc tgaacatcat ggacaaggaa aaagagctga
      481 tcgtaaaagc gctgaaaaaa cacaagggaa aacgcaagga tgccgcgtcc gatctgggca
      541 tcagcgagag gacactctat cgcaaactaa aggaatacga cattaatgaa taaggttaag
      601 atcaccgggg cgttcatttt cgccgtcgtc gccatcgttc tggcctccgg cctttcttcc
      661 tgtcacgtct attcttttaa ggacgtctcg atcccgcccg aggtgaaaag catccatctc
      721 tcctatatcg agaaccgcgc tccgctggtc aatccgcagc tggcgccgca gctcaatgac
      781 gcgttgcggc aaaagatcaa taaccaggct tcgcggttgt cccaggtaca aacttccgac
      841 gcagattatg acgtcagcgg ctgggtgtcg ggatacagct attcaacttc gggcatctcg
      901 aatcagcagg cttccagcaa ccgtctgaca gttacggttc atatcgtttt caagaaccac
      961 ctcgatccta caggaaaaaa ggtggcgccg gcggatttcg aaacggatgt gtcgaggaat
     1021 ttcgattttt ccgcatctgc cagtatcacc gatgcagagc agcagctgat gccgaccatc
     1081 atcagcaacc ttaccgatga aatctttaac aagcttttct ccaattggta gtaatgagtc
     1141 ctatgaatca accattgcag tcgatagtcc agcatctttt ccaggtgacc agcctggatg
     1201 atgtcagccg gcagcgtctg gagtcttttg tggtggagta tccctctttc ggcatcgggc
     1261 actatctgct gtcgcacaag ctccggaccg aagatcccga tcgttaccgg gacacgacac
     1321 agcggacctg tctgtatttc agtaatccat tttggctgga atggcagctc accaacagcc
     1381 cggactccga tggaccggct catgaaacgg tcgcccccgg tcattcagag acatccgtcc
     1441 ttcctgtcga agaaccccat acaattgaat cacaacaaat tgactctcaa gaagttgaga
     1501 taccaggaga tgaagtgcaa tccgctcagc cgcaatccgc tgaaccgcag ttcaatgaac
     1561 cccagtctgt cgaatccctg accgtggaca tccccgggga gacgatcgaa gaagccacag
     1621 tcgctgaacc acccgcggct cccaccgaaa caatcgtcga agaacccgct gactccaatg
     1681 aggtatctgc agcggaccag ctcttgctca gcatagaaga ggcaaggggt ttgcgcgatt
     1741 ctttgcaaaa gatcagcgaa gactttactg ttgatgcagt cccggagcag cagccgatac
     1801 aggaccagga agtccccttc gtgctcgacg agccgcaaga ggctcccgcg ccgcttcccg
     1861 aaaatccgga gcccatcgcc gcggtcaacg cagccgagcc gcaagaggcc ccgaccgagc
     1921 cgccacccgc accaaccccg gaaccggtcc ccgcccctcc tacccccgaa cccatcttcg
     1981 aaccctacca taccatcgac tatttcgcct cacagggcat ccggctaaag ctggacgaaa
     2041 atcccgccga cgctctgggc aaacagctca agagcttcac cgactggcta aagataatgc
     2101 gacgcctccc gcaaaaggat cgggattcct cggtgcccga ccgggccgcc gaacaggcgg
     2161 tgcagaactt cgccgcacat tccatccagt caaaagacat cgtcaccgag acaatggcag
     2221 aggtgctgat caagcagggt atgcgcgatc gcgccagagt catttacgag aaattaagtt
     2281 tgctcaatcc tgataaaaag gcttattttg cagccaaaat cgaacaaata aacattccct
     2341 gacatgattt tctttgtgat cctgatcgtt cttgcctgtg tgattctcgg actgatcatt
     2401 ttggtgcaaa accccaaggg gggcggtttg gcaggtaatg taggcggttt cagcaatcag
     2461 ctgatggggg taaaacagac cactgacgtc ctggaaaaag ggacctgggt actcgccgta
     2521 gtcgtggcgc tactgagtct tttttccacc gttttcgtgc ccaaggccac caacggccgt
     2581 gacatccaga aggtgaatgt accgatgcag cagcagtcta ggccctaagc
//
LOCUS       DASYSV010000007         9308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1793964, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000007 DASYSV010000000
VERSION     DASYSV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9308
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793964"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..3771
                     /locus_tag="VGQ51_00200"
     CDS             100..3771
                     /locus_tag="VGQ51_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEV9034997.1"
                     /translation="MRSTLFRRCVPATIFLLFLALSLPAQQHIEKRYPTLLWEITGHG
                     LKKPSYLFGTMHVSSKLVFHLSDSFYLDIRNADVVALELDPQLWQDQLFRFEKLQSNL
                     RYYTQGAPSDLINEKTFQLQRYEDRLRSAISDEPTVINGLLYRTFRSQADFEEDTYLD
                     LYIYQTGRKLGKQATGVENYFQTERLIMEAAQDMMKDKRKKAPDTDGESMFEIERKTQ
                     EAYRKGDLDLLDSLERLMEPSESYMEKFLYRRNEIQAASIDSIVQRHSLFVGVGAAHL
                     PGNRGVIELLRKKGYTLRPVMMPDRDATRRDDIDKIRVPVTFSTSSAPDGSFSVQLPG
                     KLYRRIDGRLSDSWQYADMGNGAYYMIGRVRTNAGFLGQREATVLRKVDSLLYENVPG
                     KILKRTPVTRNGYNGYDITARTRRGDIQRYNILVTPGEVWVFKMSGTNNYVEGPEAET
                     FFNSIRIHPVTMSGWADFTPDRGGFTIRMPRIPAQNRTTPEPEGITRWDYEATDSTTG
                     DGYLLWKSTVQNYRFLEEDTADLALMEESFRGSDWIDKRLSRRTGTFDGHACIDAGYQ
                     QKDGSFIHARFILHGPDYYILAVHSREKNKPFTGWFNSLTFVPYRYTAFRNYVDSFLH
                     ISVTTPVVPDVDAGIRGIIERGSSEEFLNSLPDYNNYWPRPKTALFQDDSTGEAIFVS
                     VESFPKYYYPRDTATFWQEEAGEKRYRRDLVIARKEPWHDGDLSGYRYTLTDTNTTRC
                     IHVWVFLQKDRIFRVVSLTDSLERNSEFLRRFYASFRPLGNPTGSGVFDSKLDIFFHD
                     FYSGDSSLAKKARAAIPNVYFGPAGVPALLSAIRTLPYNGTDYFETKTRLINELGFIS
                     DSAATPSVVAGLRDVYQRVGDTSTFQNAVFKALAHQKTTEAYRLLGQLMTQDPPVFDN
                     NSDYNYLFKDIGDSLRLARTLFPGLLQLSTVDDYKPNIQSLLADLVDSGYLKAADYEG
                     YFSQIYFDAKIQWKKQEGKDEKRLQKKDEDGDDNADAESDDNNNELDDYAILLLPFYG
                     RNPAIPHFFDRLLQSRDAGLRMNTALLLLRNRMPVADSILVALAADDASRGPLFQGLT
                     DIGRQDIFPRQFRNQESIARSLLVSGHGVDSFAAIAFVDKQPAQFKQKTGSVYFFKYK
                     INKEDDWQIGLSGIQPLGRRDISTEPGLVVTTGKRLRPGVPVLQQFEEQWRRLLLARR
                     RSAVAFFQDNEYAVRQDED"
     gene            complement(3768..5600)
                     /locus_tag="VGQ51_00205"
     CDS             complement(3768..5600)
                     /locus_tag="VGQ51_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9034998.1"
                     /translation="MSPLLSIKHLGIAFGHDGKLTQAVSDISFDVHRGEIVALVGESG
                     SGKSVTSLSILRLLPSPPARYTSGEILFTGREGGTIDLLQQQPESLRTIRGAEIAMIF
                     QEPMTSLNPVFTCGDQVAEAIRVHRKISAAEARRQTIVLFAKVELPDPEGMYSRWPHQ
                     LSGGQKQRVMIAMAMSSRPSLLICDEPTTALDVTVQKTILQLIKQLQETEDMGVIFIT
                     HDLGVVAEIADRALVLYKGSVVEEGPVRQLFSHPRHPYTKGLLNCRPALHVKGERLPV
                     VSDYLEGTMPDTARGEPAREVGTEVSRRVEREVSREVGREVELGVEREVGREVGRGVE
                     RGVERRPSAGGALVKVEDLRVWYPSKKTLFGKVLQYTRAVDGVSFEVREGETLGLVGE
                     SGCGKTTLGRALLRLVEPTGGKIIFRGAELGALRMEELKKLRSSMQLIFQDPYSSLNP
                     RMTVGAAIAETMKVHGLGGNPRQRREKVIQLLERVSLNAEHFDRWPHEFSGGQRQRIV
                     IARALALEPSFIVADESVSALDVSVQAQVLNLLNDLKAAFGFTVVFISHDLAVIRYIS
                     DRIVVMNKGKIEESGAAEDIYQHPKSDYTKKLIAAIPGVAAGAP"
     gene            complement(5638..6408)
                     /locus_tag="VGQ51_00210"
     CDS             complement(5638..6408)
                     /locus_tag="VGQ51_00210"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEV9034999.1"
                     /translation="MKTKEEIVNNWLPRYTGEQLEHFGEYILLTNFDNYVHLFADWHN
                     VKVIGEGKPMQCATAGDITIINFGMGSPGAATIMDLLTAIKPKAALFLGKCGGLKKRN
                     KLGDLILPIAAIRGEGTSNDYFPPEVPALPAFALQKAISTTIREYGVDYWTGTVYTTN
                     RRVWEHDEVFKEYLQKIRAYAIDMETATIFSVGFYNKIPTGALLLVSDQPMVPEGVKT
                     AESDLKITSEYVERHIRIGIDSLKQLINNGVTVRHLRF"
     gene            6437..6880
                     /locus_tag="VGQ51_00215"
     CDS             6437..6880
                     /locus_tag="VGQ51_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEV9035000.1"
                     /translation="MIKIYYPSHPFKIREPEKKKEEIWDELRKQWVRLTPEEWVRQNF
                     IQYLLQVKNYPASYIAVERRMRLGELNKRFDLLIFDNGAQPWMLVECIAMEERLDSRV
                     LWQVLRYNMAIPVKYIVITNGEQCHAFIRGLTDFEDIAALPAYSE"
     gene            complement(6985..8103)
                     /locus_tag="VGQ51_00220"
     CDS             complement(6985..8103)
                     /locus_tag="VGQ51_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEV9035001.1"
                     /translation="MRSFFCWSLALSVCCSARAQKYIPSLRFFPATDRRIEYMGRVER
                     SDSGTVRFWSPGVMVRLKIRSQGLIFLRDQVPNDGTHNYIEIMIDDRAALRIKLSRPD
                     DSLLIRANGSVDLNGRQIAVGEDMHSESEHVISLCKDTESGIGWLEITGVKTEALLRP
                     PALPKRKIECIGNSITAAAGMDQSQIPCGKGEWYDQHNAWMSYGAETARKLKAQWHLT
                     AVAGIGLVHSCCDMGIVMPEVFDKIDLRDNTGSWDFSLYVPDVVTVCLGQNDGIQDST
                     LFCGAYVRFLSDIRRRYPEARIVCLTSPMGDKKLTDMQKRYLEAVVAAMRAGGDRLVS
                     RYFFSRQYSNGCGGHPDLEEHGQIAGELTGYLKRLMGW"
     gene            complement(8109..8957)
                     /locus_tag="VGQ51_00225"
     CDS             complement(8109..8957)
                     /locus_tag="VGQ51_00225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV9035002.1"
                     /translation="MSDSTTRFSDRVEDYVKYRPHYPEAVVEYLRATYGLKPEWSIAD
                     IGSGTGISTELFLRNGNRVYGIEPNRGMREKGEQLLAGYARRRGEGGRGAEGRGSGGR
                     GAEDAFQGEGGGNFISIEGTAEATGLADGSVDMIVAGQAFHWFDPVQSRGEFARILRS
                     GGVVVLIWNERLIVSPFEKEYEDLILRHASDYNAINHKNISDGQFREFFHPAAFALAI
                     FPNEQLFDFEGLKGRLLSSSYIPKEGSRHVELMRGLRELFDRHAAGGRVRVGYDTKVY
                     SGVLKP"
     gene            complement(8967..>9308)
                     /locus_tag="VGQ51_00230"
     CDS             complement(8967..>9308)
                     /locus_tag="VGQ51_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEV9035003.1"
                     /translation="VTVSYFEWLKNLSHVRYGRMEKRFTENMNTHILNQIEGLTGKKV
                     SQKEKEFITHGADEADLVYSGLEETMIAATREIMDAWKSNPQIPDMRTAAYVVAINKV
                     GTSYAELGIFP"
BASE COUNT         2077 a         2888 c         2326 g         2017 t
ORIGIN      
        1 gaagggggac cccgggggag gggccgccgc tcaccgattt tagcctgcct ttagtataat
       61 aattttttcg atttggcgtc ttttacgcaa cttgtatcaa tgagatcgac cctgttcagg
      121 cgttgtgtac cggcgaccat ctttctgctt ttcctggctc tttccctccc ggcccaacag
      181 cacatcgaaa aaaggtatcc cactctcctg tgggagatca ccggtcacgg tctcaaaaaa
      241 ccctcttatc tctttggcac gatgcacgtc agcagcaagc tggtattcca tctcagcgac
      301 agcttctacc tggatatcag gaatgccgat gtcgtggcgc tcgagctgga tccccaattg
      361 tggcaggatc agctgttccg gttcgagaaa cttcaatcca atctccggta ttatacacag
      421 ggtgcgccca gcgatctcat caacgaaaaa acctttcagc tgcagcgcta cgaagaccgg
      481 cttagatccg ccatcagcga cgagcctacg gtcatcaacg gcctcctcta ccgcaccttc
      541 cgcagccagg ccgatttcga ggaagatacc tatctcgatc tctatatcta tcagaccggc
      601 cgaaagctcg gcaaacaggc caccggcgtg gaaaactatt tccagaccga acgtctgatc
      661 atggaggccg cacaggacat gatgaaggac aaacggaaaa aggcgcccga taccgacggg
      721 gaatcgatgt tcgagatcga acggaaaaca caggaagcct atcggaaagg cgatctcgac
      781 ctgctcgact cgctcgagcg gctgatggaa ccctccgagt cctatatgga gaaattcctt
      841 taccggcgca acgagatcca ggcagcctcg atcgatagca tcgttcaaag acattcgctc
      901 tttgttgggg taggggccgc ccatctgccg gggaacaggg gcgtcatcga actgttgcgc
      961 aaaaaaggat ataccctccg gcccgtgatg atgcccgacc gggacgcaac acgccgggac
     1021 gatatcgaca agatcagggt acccgtaacc ttctcgacgt cgtctgcccc ggacgggagc
     1081 ttttcggtgc agctgccggg taagctctat cgccgcatcg acggccgttt gtccgactcc
     1141 tggcagtatg ccgacatggg caacggcgcc tactatatga tcgggagggt gcggacgaat
     1201 gccggatttc tcggacaaag agaggcgacc gtcctccgga aagtggatag cctgctctac
     1261 gagaatgtcc ccggaaaaat actcaaaagg acaccggtga cccgcaacgg ctataatgga
     1321 tatgatatca cggcccgtac ccgcagaggc gatattcaac ggtataatat cctcgtgaca
     1381 cccggggaag tatgggtctt caagatgagc ggtacgaaca actatgtcga aggtcccgaa
     1441 gccgaaacct ttttcaacag tatacgcatt catcccgtaa caatgtccgg ctgggcagac
     1501 ttcacccccg accggggcgg atttacgatc cgcatgcctc gtatccccgc gcagaacagg
     1561 accacgccgg aacccgaggg tattacccgc tgggattacg aagccaccga ctccaccacc
     1621 ggtgacggct acctgctatg gaagagcacg gtgcagaact accggttcct cgaagaagac
     1681 accgccgatc tggcgctgat ggaagaatcc tttcggggtt cggactggat agacaagcgt
     1741 cttagccggc ggaccggcac tttcgacggt catgcctgta tcgacgccgg ctaccagcaa
     1801 aaggacggct cctttatcca tgcccggttc atcctccacg gtccggacta ttacatactt
     1861 gcggtacaca gccgggaaaa gaacaaaccg tttaccggct ggttcaactc gctgaccttc
     1921 gtcccctatc gctataccgc cttccgcaat tatgtggatt ccttcctgca tatcagcgtc
     1981 acgactcccg tagtaccgga cgtcgacgcg ggtatccgcg gcatcatcga aagaggcagc
     2041 agcgaggagt tcctgaactc ccttcccgac tacaacaact actggccgcg tcccaagacc
     2101 gctctattcc aggatgactc taccggggag gccatattcg tatccgtgga gtcgtttccc
     2161 aaatactatt atccccggga tacggccacc ttctggcagg aagaagcggg cgaaaaaaga
     2221 tacagacgcg atctggtcat cgcccggaaa gaaccctggc acgacggcga tctttccggc
     2281 taccgatata ccctcaccga taccaatacc acccgctgca tccatgtatg ggtattcctg
     2341 caaaaggaca gaatattccg agtagtatcg ctcaccgact cgctggaacg gaacagtgaa
     2401 ttcctccgcc gattttatgc cagcttccgg cccttgggga atccgaccgg atccggcgtc
     2461 ttcgatagta agctggacat ctttttccat gatttttata gcggtgactc ctcgctggct
     2521 aaaaaggccc gggcggccat acccaacgtc tatttcggac cggccggtgt ccccgcattg
     2581 ctcagtgcca tccggacact gccctacaac ggaacggact acttcgaaac aaagacaagg
     2641 ctgatcaacg agcttggttt tatcagcgac tctgccgcca ctccgtccgt cgtggcgggt
     2701 ctccgtgacg tttaccagag ggtgggcgat acgagcacct ttcagaatgc cgttttcaag
     2761 gcgctggcgc atcaaaagac gaccgaagcc taccggctgc tgggacagct gatgacacag
     2821 gacccgccgg tgttcgacaa caactctgac tataactacc tgttcaagga tatcggcgat
     2881 tcgctgagac tggcccgtac cctgtttccc ggtcttctcc agctgtcgac ggtggacgac
     2941 tacaaaccga acatccagtc cctgctggcc gacctcgtcg acagcggcta tcttaaggcc
     3001 gccgactatg aaggctattt cagccagatc tatttcgacg ccaagatcca atggaaaaaa
     3061 caggagggaa aggacgagaa gaggctccag aaaaaagacg aggatggaga cgacaacgcc
     3121 gatgcggaga gcgatgacaa taacaatgag ctcgatgact atgccatact tctccttcct
     3181 ttctatggaa ggaatccggc catccctcat tttttcgacc ggctgttaca aagccgcgat
     3241 gccgggctga ggatgaatac agccctgctg ctccttcgca accggatgcc tgttgcagac
     3301 tcgatactgg tcgcactggc ggcggacgat gcctcgcgcg gaccgctgtt ccagggtctc
     3361 actgacatag gccggcagga tatcttcccc cggcaattcc gcaaccagga atccatcgcg
     3421 cggagcctgt tggtctccgg acatggcgtc gatagttttg cggcgatcgc tttcgtcgac
     3481 aaacagcccg cgcagttcaa acaaaagacg ggatcggtat atttcttcaa gtataagatc
     3541 aataaggagg acgactggca gataggcctc agcggtatcc agccgctagg gcgccgggat
     3601 atcagtaccg aaccgggcct ggtggtgacg acgggaaaaa ggcttcgccc tggcgtacct
     3661 gtattacagc aattcgagga gcagtggcga cgattgttac tggcacgtag gaggagtgcg
     3721 gtcgccttct tccaggataa tgagtatgcg gtgagacagg atgaagatta gggcgcaccc
     3781 gccgccactc ccggaatcgc cgcaatgagt ttcttcgtgt agtccgattt tggatgctgg
     3841 taaatgtcct ccgccgcgcc cgactcttct atcttcccct tattcataac cacgatccta
     3901 tcgctgatat agcgtatcac cgcgagatca tgcgatatga agacgacggt aaacccgaat
     3961 gccgccttca gatcattgag cagattgagc acctgcgcct gcacgctgac atccagtgcc
     4021 gaaacggact cgtccgcaac gatgaacgaa ggttcgagtg cgagcgcgcg tgcgatcacg
     4081 atgcgctgcc gctgacctcc cgaaaattca tgcggccagc ggtcgaaatg ttcggcattc
     4141 aggcttaccc tttccaatag ctggatcact ttttccctcc tctgtcgcgg gttgccgccc
     4201 aatccatgga ctttcatcgt ctctgcgatg gcggcgccga ccgtcatccg gggattcagc
     4261 gaagaatagg ggtcctggaa gatcagctgc atgctggacc gcaatttctt cagctcctcc
     4321 atccgcaaag cccccaattc tgcacctctg aaaataatct tcccgcctgt gggttcgacc
     4381 agcctcaaca atgctcttcc aagcgtcgtc tttccgcaac ccgactcgcc gaccagcccc
     4441 agcgtttcgc cttccctgac ttcgaaggat accccgtcga ctgcgcgggt atattggagt
     4501 actttcccaa ataatgtttt tttcgaaggg taccagaccc gcagatcctc gactttcacc
     4561 aacgccccgc cggccgatgg ccgccgctcc accccccgct ccaccccccg ccccacctcc
     4621 cgccccacct cccgctccac ccccagctcc acttcccgcc ctacctcccg cgaaacttcc
     4681 cgctctaccc gccgcgaaac ttccgtcccc acctcccgcg ccggttcgcc tcgggcggta
     4741 tccggcatcg ttccttcgag ataatcgctg acgaccggca accgttcgcc tttcacatgt
     4801 aaggcagggc ggcagttcag cagccccttc gtatacggat gtcgcggatg gctgaaaagc
     4861 tgccgtacgg gcccttcctc taccacgctc cccttgtaca gcaccagtgc cctgtcggca
     4921 atttccgcga cgaccccgag atcatgggtg ataaagatca cccccatatc ttcggtttct
     4981 tgaagctgtt tgatcagctg gagaatggtt ttttgcaccg taacgtcgag cgcagtggtc
     5041 ggctcgtcgc agatgagcag ggaaggccgg ctgctcatgg ccatggcgat catgacccgc
     5101 tgtttctgtc ctccgctgag ctggtggggc caccgcgagt acatcccttc cgggtccggc
     5161 agctccacct ttgcgaacaa aacgatcgtc tgccgtctcg cttcagccgc ggatatttta
     5221 cggtgaactc ttatagcctc ggcaacctgg tcgccacagg tgaagactgg attcagcgag
     5281 gtcataggct cctggaagat catcgcgatc tcagctccgc gaatggtgcg caatgattcc
     5341 ggttgctgct gcaacagatc gatcgtcccg ccctctcttc ccgtaaacag gatctctccc
     5401 gatgtatagc gcgccggcgg tgagggcagc aatcgcagga tggacagtga ggtcacggat
     5461 ttccccgaac ccgattcgcc cacgagggcg acgatctcgc cgcgatgtac gtcgaagctg
     5521 atgtcggata cggcctgggt caatttgcca tcatgcccga aggctattcc cagatgtttg
     5581 atcgatagga gcggagacat agaacagcta aaataaggaa tttagcagcg cgccgcttca
     5641 gaaccgcaga tgccgcaccg tcaccccatt attgatcaat tgtttcaacg agtcgatgcc
     5701 gatgcggatg tgacgctcta catattcgga cgtgatcttc agatcgcttt cggccgtctt
     5761 gaccccctcc ggcaccatcg gctgatcgct taccagcaac agcgcccccg tcgggatctt
     5821 attgtaaaaa cccaccgaaa agatcgtcgc ggtctccata tctatcgcat aagccctgat
     5881 cttctgcagg tattccttga atacctcatc atgctcccat acccgccggt tggtcgtata
     5941 gaccgtcccc gtccagtagt cgacgccata ttcgcggata gtggtcgata tcgccttttg
     6001 cagcgcaaac gccggaagcg caggcacctc cggaggaaaa tagtcgttgg aagtaccctc
     6061 cccgcgtatg gccgcgatgg gtaggatcag atcgcccagc ttattccttt ttttgagacc
     6121 gccgcacttc ccgaggaata acgccgcctt cggcttgatg gccgtcagaa ggtccatgat
     6181 cgtcgcggcg ccgggactgc ccatgccgaa attgatgatg gtgatatccc cggcggtggc
     6241 gcattgcatc ggtttaccct caccgataac cttcacatta tgccagtccg cgaaaagatg
     6301 cacatagttg tcgaagttag tgagcaggat atactcccca aaatgctcca gttgctcgcc
     6361 tgtatatcgg ggcagccagt tattaactat ctcttctttg gttttcattt attaaattta
     6421 ccaaatagtt ttggctatga ttaagatcta ttatccttct catccattca agatcaggga
     6481 gccggagaag aagaaggagg agatatggga cgaactccgc aaacagtggg tacgcctcac
     6541 gccggaagaa tgggtccgcc agaactttat tcaatatctc ctgcaggtga agaattaccc
     6601 ggcctcctat atcgccgtcg aacgccgcat gcggttgggc gagctgaaca aacggttcga
     6661 tctcctcatc ttcgacaacg gtgctcagcc ctggatgctc gtcgaatgca tagccatgga
     6721 agaaagactg gatagccggg tcctctggca ggtgttgcgt tataacatgg ccatccccgt
     6781 aaaatatata gtgatcacca acggcgagca atgccatgcc ttcatccgcg gtctcaccga
     6841 tttcgaagat attgccgcat tgccggcata tagcgaatga tcaccaacga cacccttctc
     6901 attccccgcc aatccctttc attccccgcg cccccggcat cctcaccaac caatcccttt
     6961 cattccccgc gccccccggc atcctcacca acccatcagc cgcttcagat aacccgtcaa
     7021 ctcacccgcg atctgcccat gttcctccag atccggatgc cccccgcaac cattcgaata
     7081 ctgacgcgaa aaaaaatatc tgctcaccag tcggtcgcca ccggcccgca tagccgccac
     7141 gacggcctca agataacgct tttgcatatc cgtcaacttc ttgtctccca ttggactggt
     7201 cagacaaacg atccgcgctt ccggataccg gcgccggatg tcactgagaa accgcacata
     7261 ggcgccacaa aacaacgtcg aatcctgtat gccgtcattc tgcccaagac ataccgtgac
     7321 cacgtccggc acataaaggc tgaaatccca ggagcccgta ttatcccgta gatctatctt
     7381 gtcgaatacc tccggcatca cgatgcccat atcgcaacag ctatgcacca gcccgatgcc
     7441 cgccaccgcc gtcagatgcc actgcgcttt cagcttgcgc gccgtctccg cgccatagct
     7501 catccaggca ttgtgctgat cgtaccactc gcctttcccg caggggatct gagactgatc
     7561 cataccggcc gctgcagtga tcgagttgcc gatacattcg atctttcttt tgggcagtgc
     7621 gggaggacgc aaaagcgctt ccgtcttcac gccggtgatc tccagccagc caatacccga
     7681 ttcggtgtcc ttgcaaagac tgatcacatg ctcggattca gaatgcatgt cctcgccgac
     7741 ggctatttgg cggccgttga ggtctacact gccattcgcc cggatcagca ggctatcgtc
     7801 tggccggctc agctttatcc ggagggccgc ccggtcatcg atcatgattt cgatataatt
     7861 atgcgtcccg tcattgggga cctggtcacg cagaaagatc agaccctggc tgcggatctt
     7921 caatcgcacc atgacgccag gcgaccagaa acggacggtc ccggaatcgg aacgctccac
     7981 ccggcccata tattcaatac gcctgtcggt cgccgggaag aacctaagcg atggaatata
     8041 tttttgtgcc cgggcgctac agcagaccga cagcgccaat gaccagcaga agaatgatct
     8101 catgtaattt atggtttgag gacgcctgaa tagaccttcg tatcataacc cacacgcaca
     8161 cgcccgcccg ccgcatgccg gtcgaacaac tcccgtagcc cgcgcattaa ttccacatgc
     8221 cgcgacccct ctttcggtat ataggacgac gatagcaacc ggccctttaa tccttcgaag
     8281 tcgaacaatt gctcatttgg gaagatcgcg agcgcaaaag ccgcaggatg aaagaattcc
     8341 cggaactgcc cgtccgatat attcttatga ttaatggcat tgtagtcgct ggcatgccgc
     8401 aggatcaggt cctcatattc cttttcgaaa ggcgacacga tcagcctttc attccagata
     8461 aggacaacga caccgccaga ccgcaatatc cgcgcaaact ccccgcggct ttgcacggga
     8521 tcgaaccaat ggaacgcctg tcccgccacg atcatgtcga cactgccgtc cgccagaccc
     8581 gtggcttccg ctgtgccttc gatgctaatg aaattgccgc caccctcgcc ctggaatgca
     8641 tcttccgcgc cccgtcctcc cgagccccgt ccttccgcgc cccgtcctcc ctctccccgt
     8701 cgtcgcgcat accccgccag cagctgctct cccttctccc gcatcccgcg attcggctct
     8761 atcccatata cgcgattgcc attgcgcagg aacagttccg tagagatgcc cgttcccgaa
     8821 ccgatgtcgg caatactcca ttcgggctta agcccgtagg tggcgcgcag atattccacg
     8881 acagcctccg gatagtgagg gcgatatttg acataatcct cgacgcggtc gctgaaacgc
     8941 gtggtactgt cggacatacg tctaatttac gggaaaatcc ccagctctgc gtaggaggtg
     9001 ccgaccttat tgatcgccac gacataagcc gcagtacgca tgtcggggat ctggggattg
     9061 ctcttccacg catccatgat ctcgcgggtg gccgcgatca tcgtctcttc gagaccgcta
     9121 tacacgaggt ccgcctcatc ggcgccatga gtgatgaact ctttttcctt ctgcgacacc
     9181 tttttccccg taagcccttc tatctgattc aggatatgcg tgttcatatt ttcggtgaag
     9241 cgcttttcca tccggccata ccgcacatgg ctgaggtttt tcagccattc gaaatacgag
     9301 acggtcac
//
LOCUS       DASYSV010000008         6207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2306760, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000008 DASYSV010000000
VERSION     DASYSV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2306760"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VGQ51_00235"
     CDS             <1..1221
                     /locus_tag="VGQ51_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEV9035004.1"
                     /translation="NALFEHATEGMVLTNDKGRIVLVNPAACHLFGYGHGELLGQSIE
                     VLIPQRFHSSHEHYRNGFYHQPGNRTMGHGRDLFARKKDSSEFPVEVSLSYYRQKNVL
                     FVIAFIVDITQRKQSELELVHRQLELEHRTEDIRKLNAGLESKVEERTLILQEALQEL
                     ERSQRELSEALDKERQLNEIKSRFVSMASHEFRTPLSTVLSSAALLSKYTRADEQDRR
                     DKHIRRIKESVKHLNNLLEDFLSLGKLEEGKIKVEPGEFPIREFLEEVVEEMRPLAKD
                     GQQIEFEYAGSNGFVTDKRLLKNILINLLSNAIKFSPEAATIYLQAKHTENAGLSLSV
                     KDQGIGISEEDQQHLFSSFFRGANALNIEGTGLGLHIVKRYADLLQGHLLLESELGKG
                     TTITIELPGSEEPG"
     gene            complement(1278..2102)
                     /locus_tag="VGQ51_00240"
     CDS             complement(1278..2102)
                     /locus_tag="VGQ51_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV9035005.1"
                     /translation="MLASLQNPFVIILFVIMILLGIVITLLASALPGRAGVRKNGAGE
                     GKEAADPAGPAGMSKTKLGSAAGIAGAAAGTGLFLPAAGGVSDTVFYISVGVIVLELL
                     VILALWYNLWIISAVGGKAEPRMDGRFAFVPAPRWWRFYGVFGLLLIAVACLWGYRVL
                     STPRVVDERTVKALTSPKDLAAGRTVFQGTCFVCHGKNGEGGIGPNLTDGYWIHGGSI
                     QDIFRTIKYGWPDKGMKSWKDDYSPEQIAQTASYVKSLSGTHPANPKPPQGNLYKP"
     gene            2244..3368
                     /locus_tag="VGQ51_00245"
     CDS             2244..3368
                     /locus_tag="VGQ51_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9035006.1"
                     /translation="MRRLLVIDDHDDIRENIAEILTLAGYEVYTAENGKRGVETALKE
                     NPELVICDIMMPELDGYGVLHLLRKNESTINTPFIFLTARTERTDFRKGMEMGADDYI
                     TKPFDDIELLNAIEIRLKKYDILQNSYAADEKGAGALIGDLSTSGMIPLDPANYETTE
                     LSKKQHLYQEGRRPKYAYYLIKGKVKAFRIHEDGKEYITNLYGPGDYIGYLPLLENSP
                     YEETAIVLEDAELAQIPKDEFVNALYQDIRIASKFIRLIAQNVQEKEERLLQLAYGSL
                     RKRVAKALIDIHNKYAAAGTASTATDATSAGKGATSPAKGAPPSAPLDISRDDIAQYV
                     GTATESLIRTLSDFKSEKLVDIRDGKIRIADLAKLQNLLY"
     gene            complement(3369..3593)
                     /locus_tag="VGQ51_00250"
     CDS             complement(3369..3593)
                     /locus_tag="VGQ51_00250"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEV9035007.1"
                     /translation="MRNENEILEEVKLRVNSILCPGTIDVAVDGNIAVLTGHVPSLEK
                     RNEVAMTAAYVSGIEGVENKLTISAPEYIL"
     gene            complement(3626..4963)
                     /locus_tag="VGQ51_00255"
     CDS             complement(3626..4963)
                     /locus_tag="VGQ51_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV9035008.1"
                     /translation="MFKIKYVTAEEAVSVVKSGDRVFIHGSAATPVPLVTALQARHKE
                     IRQVELVSITTMGDLDFDNPLWCDSFFVNSLFVSGSTRKVANSDHGDYVPIFLSQIPQ
                     LFKRGFLPIDVALIQVSQPDKHGFCTLGTSVDIARAALDTARHIIAQVNPNMPRTHGD
                     GFIHVKDIDALVWHEMRLPEVNYSGEANPAVEKIGRHVASLIPDGATLQLGIGHIPDQ
                     VLRNLAGHKNLGLHTEMLSDGVIPLIESGVIDNRCKKLNVGRSVTSFVNGTRRLFEFI
                     DDNPEIRVMDIGYVNDTAVIRQNPKVAAINSAIEIDLTGQVCADSIGTLQYSGIGGQM
                     DFIHGASLSEGGLPIIAMPSVTSTGVSRIVPYLKEGAGVVTTRGHVHWVATEYGAVNL
                     FGKNFRQRAEALIGIAHPEHREWLDRQFYQRCHRGAAVILPHSEPRPEPAAGL"
     gene            5093..5914
                     /locus_tag="VGQ51_00260"
     CDS             5093..5914
                     /locus_tag="VGQ51_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV9035009.1"
                     /translation="MRSIVVPVNFTENSACAARYAADMAAAIRADLHLIYVFQPPVSA
                     AEIPLPESVYDEMRDSGIELLDDLSKELRRRTADTVKVYTDMEIGGVGPRLEAFCKFR
                     NPFLVVMGSSGHGVENILQGSHTVQAMRHLHYPLLVVPPKTHWTPVQKIVVACDKEDI
                     DGGMPGTLPFLDELSQLLGARLEVVHVITNGEASPSEAIAEYNVWKKDVKALVPELHF
                     VRQSKVQSGVSEYLENHKADWLMVFPKSHSFLEFHKSRARQIVLSCAIPVMSVHE"
BASE COUNT         1589 a         1909 c         1445 g         1264 t
ORIGIN      
        1 aatgccctgt tcgaacatgc taccgaaggc atggtactga ccaacgacaa gggcaggatc
       61 gttttggtga acccggccgc ttgccatctt ttcggatacg gccatggaga actgctcggt
      121 caatcgatcg aggtgctgat cccgcaacga ttccattcct cgcatgaaca ctatcgcaac
      181 ggcttctatc accaacccgg caaccgcacc atgggccacg gccgggacct ttttgcccgg
      241 aaaaaagact cttccgaatt ccctgtcgaa gtgagcctca gctattaccg tcaaaagaat
      301 gtgttgttcg tcatcgcctt tatagtggat atcacccaac ggaaacagtc cgaactcgaa
      361 ctggtacacc gccaactgga actcgaacac aggacggagg atatacgtaa gctcaatgcg
      421 ggcctggaat cgaaagtgga agagcgtacc cttatcctcc aggaagccct ccaggagctg
      481 gaacgttcgc aacgcgagct gagcgaagca ttggacaaag aaagacagct caacgagatc
      541 aaatcccgtt tcgtttccat ggcttcacac gaattccgca ctcccctgag caccgtgctt
      601 tcctcggccg ccctcctttc caaatatacc cgggcagatg agcaggaccg ccgcgataaa
      661 cacatccgcc gtatcaaaga gtccgtcaaa cacctcaaca acctgctcga agactttctc
      721 tcgctgggca aactcgaaga aggcaagatc aaggtggaac ccggcgaatt tccaatcagg
      781 gaatttctcg aggaagtggt cgaagaaatg cgcccgctcg ccaaagatgg ccagcagatc
      841 gaattcgaat atgcgggttc gaatggcttc gtcacagaca aacgcctcct caaaaatatc
      901 cttatcaacc tgctgagcaa tgccattaaa ttctctcccg aagcggcgac catctatctc
      961 caggctaaac ataccgaaaa tgcaggacta agtctctcgg ttaaggacca gggcatcggc
     1021 atttcggaag aggaccagca gcacctgttc agcagcttct tccgcggtgc aaacgccctc
     1081 aacatcgaag ggacgggtct cggcctgcat attgtcaaac gctacgccga cctcttgcaa
     1141 ggtcacctcc tcctggaaag cgaactcgga aaaggcacca ctattaccat tgaattgccc
     1201 ggttctgaag aaccaggcta aactattgcg gggaagccga ccgcccccat tgcctaactt
     1261 cccattacag tcggagatca gggcttataa agatttccct gcggcggttt gggatttgcc
     1321 ggatgcgtcc cgcttaggct tttcacatag ctggctgtct gcgcaatctg ctccggggaa
     1381 tagtcgtctt tccagctctt catccccttg tcaggccacc cgtatttaat ggtccggaaa
     1441 atgtcctgga tgctgccccc atgtatccaa tacccatccg taagattcgg gcctatccct
     1501 ccctccccgt ttttgccatg acagacgaaa caggtcccct ggaatacagt ccttcccgcc
     1561 gcaagatctt tcggtgaagt gagtgctttt accgtccgtt cgtcaaccac cctgggcgtg
     1621 ctcagtaccc ggtaccccca aagacaggcc acggcgatca aaagaaggcc gaaaacccca
     1681 taaaacctcc accacctggg ggccgggaca aaagcaaatc gtccatccat ccgcggctcg
     1741 gcttttccgc caacagcaga aatgatccac aggttgtacc ataacgccag gatcacgagc
     1801 aattccagca caatgacacc gacactgatg taaaagacgg tatcggatac accaccggca
     1861 gccggtaaaa acaacccggt acccgcggcg gcccctgcaa ttccagccgc actacctaat
     1921 tttgtctttg acatgcccgc aggacctgcc ggatcggcag cctccttacc ttccccggcc
     1981 ccgttcttgc ggacacctgc cctgccaggc agcgcgctgg ccagcaacgt aatgacaata
     2041 ccaaggagga tcataatgac gaacagaata atcacaaagg ggttttggag ggatgccaac
     2101 ataagagcgt tttaagaaat ggtgaacgca agctagcact ggtacactgc cagccggctg
     2161 acttccgtca tagaaaatac tgatcctggt cagttgcggg cttgccgccg attgcgtatt
     2221 attgtagcgc taaaatcacc aacatgcgca gactcctcgt aatcgacgac catgatgaca
     2281 tccgcgaaaa tatcgccgaa atactaactc ttgccggtta tgaagtatac accgccgaga
     2341 acggcaaacg cggtgtcgag accgccctaa aagaaaatcc cgaactggtc atctgtgaca
     2401 tcatgatgcc cgagctggat ggctacggcg tgctccacct gctgcgaaag aatgagtcca
     2461 ccatcaatac cccattcatc ttcctcaccg cccgcaccga acgaaccgat ttccggaaag
     2521 gcatggaaat gggagccgac gactatatca ccaaaccctt cgacgacatc gaactcctga
     2581 acgccatcga gatccgtctc aaaaagtatg acatcctcca aaacagctat gccgcagacg
     2641 aaaaaggcgc cggcgccctg atcggcgatc tcagcaccag cggaatgatc cccctggacc
     2701 cggctaatta cgagaccaca gagctttcca aaaaacaaca tctctatcag gaaggccgca
     2761 gacccaaata tgcttactac cttatcaaag gaaaagtgaa agccttccgc atccatgaag
     2821 acggcaaaga atacattacc aatttatatg gaccgggcga ttatatcggt tacctgcccc
     2881 tgcttgaaaa tagtccctat gaggaaacag ccatcgtact cgaagacgcc gagctcgcgc
     2941 agatacccaa agacgagttt gtgaacgccc tataccagga tattcgcatc gcctcgaaat
     3001 tcatccggct catcgcacaa aatgtgcagg agaaggaaga acgtctcctg caattggcct
     3061 atggctcgct tcgaaaacgc gtggcaaagg ccctgatcga tattcacaac aaatatgccg
     3121 ccgcgggcac ggcctccacc gccacagacg caacctccgc tggcaaaggc gcaacctcgc
     3181 ctgccaaagg cgcgcccccc tccgcccctc tggacatctc ccgcgacgac atcgcacaat
     3241 atgtcggcac tgccaccgag tcgctgatcc gtacgctcag cgatttcaaa tccgaaaaac
     3301 tcgtcgatat ccgcgacggc aagatcagga tcgccgacct ggcaaaattg caaaacctgc
     3361 tctactgatc acaagatata ctccggcgca ctgatcgtca acttattctc gaccccctcg
     3421 atccctgaca cataggcggc cgtcatcgct acctcattcc ttttttccag cgacggaaca
     3481 tgcccggtca atacagcgat attcccatcg accgcaacat cgatcgttcc gggacagaga
     3541 atggaattga cccggagttt cacttcttcg agaatttcat tttcgttacg catagcagta
     3601 agattttatg agtgaataaa ttggttcaaa gccccgccgc cggctccggc cgcggttctg
     3661 aatgcggcaa aatcacagcc gccccacggt gacaacgctg ataaaattga cgatccaacc
     3721 attcccggtg ctcgggatga gcaatcccga tcagtgcttc cgcccgctgc cggaaattct
     3781 ttccgaacag attgacagcg ccatactccg tcgccaccca gtgcacatga ccgcgggtgg
     3841 tcaccacccc cgccccctct ttcaggtaag gtacgatacg ggatacacct gtcgaagtca
     3901 cggacggcat ggcgattata ggcaaccccc cttccgatag tgaagcgcca tggataaaat
     3961 ccatctgccc cccgataccc gagtactgaa gcgtaccgat cgaatcggca catacctggc
     4021 ccgtcagatc gatctcgata gcgctgttga tcgccgccac tttcggattt tgacggatca
     4081 ccgccgtatc gttcacataa ccgatatcca tcacccggat ctcagggttg tcatcgataa
     4141 attcgaaaag ccgccgggtg ccgttgacaa acgatgtaac cgaacgcccc acattgagct
     4201 tcttacaacg attatcgatc acccctgatt cgatcagcgg aataaccccg tccgacaaca
     4261 tctccgtatg cagcccgagg ttcttatgtc ccgccagatt tctaagcacc tgatccggga
     4321 tatgcccgat acccaactga agcgtcgccc cgtcagggat cagcgaggca acatgccgcc
     4381 cgatcttttc aacagcggga ttcgcttccc ccgaataatt cacctccggg agacgcatct
     4441 catgccagac cagcgcatcg atgtccttca catggataaa accatctcca tgcgtccgcg
     4501 gcatattcgg attcacctgc gcgatgatat gacgagcggt atctaacgcc gccctggcaa
     4561 tatcaacact cgtcccgagc gtacaaaaac catgtttatc gggctgcgac acctggatca
     4621 gcgccacgtc tatcggcagg aagcctctct tgaacagctg cggtatctgg ctcaggaaaa
     4681 tgggtacgta gtcgccatga tcactattgg ccaccttgcg ggtgctgccc gacacgaaca
     4741 gcgaattgac gaaaaaacta tcgcaccaaa gcggattgtc aaaatccaga tcacccatcg
     4801 tcgtaatgct caccaactct acttgcctga tctctttatg acgggcctgc agcgccgtca
     4861 ccaggggcac cggcgtagcg gcgctgccgt gaataaacac gcggtcaccg ctctttacta
     4921 cggaaaccgc ctcttccgcg gttacatatt ttatcttgaa catgggatga ttttatggtg
     4981 tgaaattagt cgcgcatcgg aaagccgcta ctgacagctg tcatccctat aaatgataaa
     5041 ggtcataggc tccggcggcg gccggcacta gatttacata aaaatcaccg ttatgcgctc
     5101 catagtagtt cccgtcaact tcaccgaaaa ttccgcctgt gccgcacgtt atgccgccga
     5161 tatggccgca gccatcaggg cggacctcca cctgatttac gttttccagc caccggtatc
     5221 cgcggcagag atacccttac ccgaatccgt ctacgatgaa atgcgggaca gcgggatcga
     5281 gcttctcgac gacctgtcaa aggagctgcg cagacgcacc gccgataccg tgaaagtata
     5341 caccgacatg gaaataggcg gcgtaggccc ccggctcgaa gccttttgca aattcagaaa
     5401 cccatttctg gtcgtcatgg gcagttccgg ccacggcgtg gaaaatatat tgcaaggcag
     5461 tcataccgtt caggccatgc gtcatttaca ttacccttta ttggtcgtac caccgaagac
     5521 ccattggact ccggttcaaa agatcgttgt ggcctgcgat aaagaggata tcgatggcgg
     5581 aatgccgggc actctcccat ttttggatga gctcagccag ttgctgggcg cacgtctcga
     5641 agtggtccat gtcatcacca atggggaagc cagcccatcc gaagccatcg ccgaatacaa
     5701 cgtctggaaa aaggatgtca aagcccttgt cccggaacta cattttgtgc gccagtccaa
     5761 agttcagagt ggagtcagcg aatacctcga aaatcataag gccgactggc tgatggtctt
     5821 tccaaaaagt catagtttcc tggagtttca taaaagccgg gccaggcaga tcgtgctcag
     5881 ctgtgcgatc ccggtcatga gcgtacatga atagcgggaa ctatcggttg gttcagaagt
     5941 gaagtcactc cggtcttctg accggcgttt ttttgcccct taaacagccg aagaatagcc
     6001 gcaaaacggc acacccgaac gaatccgctc ctcacgcagc agatgaagat cgaaggcctt
     6061 gcacacaatc cgcaggaacg gccggcccat ctcagtaagc cgaatccccc gaccatccca
     6121 cgcacccgcc ccttcggacg aacccgcgcc atcccaagcg cccgccccct cccgaaaacc
     6181 ctcccccccg gacgtaccct ccccccc
//
LOCUS       DASYSV010000009         4432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_129207, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000009 DASYSV010000000
VERSION     DASYSV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129207"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VGQ51_00265"
     CDS             complement(<1..706)
                     /locus_tag="VGQ51_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035010.1"
                     /translation="MKSLLTIAYLVLIVAGIFISTRSRRNNARFYSGKKPSVFYTFFY
                     YALGLLITFLSYGVLTGIFPMAAILLLLAASILAQAIYSVAGAVLGFTLLEKKEKIRF
                     LSTISGAQHSKLVLLIVFLNALINIGFIVYILILFWRNPYRDHGTAIYIAFVQFTLSS
                     LTSSFATGLITWPLLTNEFSDDDARNNTLASNFSGILGNMIMLLFPYLLFKNDFFAEL
                     TKIGIPLPSFGMLLSIP"
     gene            843..1274
                     /locus_tag="VGQ51_00270"
     CDS             843..1274
                     /locus_tag="VGQ51_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEV9035011.1"
                     /translation="MNLVQRVERWGDTHHPLWLDFVRILLGAFLCYKGVDFLMNMGQM
                     LNLITNRMSFGTFAAMLMSNYISFAHLLGGILLILGVLTRFACLVNIPILLGAVFFVN
                     TTLYRPFSEVLVSILILLLLILFLIVGNGKMQMLTFREEKQ"
     gene            complement(1276..3060)
                     /locus_tag="VGQ51_00275"
     CDS             complement(1276..3060)
                     /locus_tag="VGQ51_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV9035012.1"
                     /translation="MMEPKRLFDCLELHLEKDPLPDMLAARENGTWKTYSTTDIATLV
                     RQLSTGLLRLGIPGGDRSVEGIDKVAVISKNRPEWIMLDMAVQRTGAVLAPIYPTISP
                     SELEFILNDAQVKYIFVNDGELFHKVSSIRDKVASLREIFTFENVKNVRHWKELLAEP
                     TAEETKRLDSIRNGIGENELATVIYTSGTTGTPKGVMLSHRNILSNVQAAIPCLPQGT
                     RRALSFLPLNHIYERMLSYLYLFSAVSVYYAESMDTIGDNLKEVKPQMFVTVPRLLEK
                     VYDRIMHKGAELTGVKKKLFFWAHGLAARYEINKSMGFAYDLQLALANKLIFSKWREA
                     LGNNIVAIVSGGAAAQVRLIRIFTAARIPILEGYGLTETSPVIAVNRMEIKDRLFGTV
                     GPIIPGIEVNIAEDGEILCKGPNVMMGYYKRPDLTTEAITDGWFHTGDIGVLVEGRFL
                     KITDRKKEMFKTSGGKYVAPLPIETKLKESPFVEQIMVVGPERKFVGALIVPAFANLK
                     AWAKQNEIADGTHEELIRNPKVLAFYREIVDSYNTNFNHVEQIKRFELLPAEWTIDTG
                     EMTPKLSLRRKVVTEKFHAAIEKIYRES"
     gene            complement(3106..4131)
                     /locus_tag="VGQ51_00280"
     CDS             complement(3106..4131)
                     /locus_tag="VGQ51_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035013.1"
                     /translation="MSRTLFLAVSLLTFAVVGQAQAQASDGKTPQEVARTYTQQGDYT
                     NAIVVLNGALQKDPRNLELSKDLSFNYYLSRDYTKGLSIAKPLIDRPDADVQTYQIVG
                     MFYKVQDDRKECEKLYRAGLKRFPRSGVLYNEYGEVLWASQDYSAIRQWEKGIETDPN
                     YSSNYYNAAKYYYFTRDKVWALLYGEIFINLESYSKRTAEMKDLLLAGYKKLFLDPDM
                     KKDQDTKNSFGMAWLNVMAPLNFTVRDGITAESLTVLRTKFIINWFNTYPSNYPFRLF
                     DYQRQLLKEGMFEAYNEWIFGAAGNLPAFQAWTNTHSDVYNRFINFQKGRIFKLPEGQ
                     YYQNAAK"
     gene            complement(4164..>4432)
                     /locus_tag="VGQ51_00285"
     CDS             complement(4164..>4432)
                     /locus_tag="VGQ51_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEV9035014.1"
                     /translation="TILQKNVLDKLLAIKDPRTDKRINFVGGIRGLGELQRRVDSGEM
                     KAAFSLYPVSIEQLFDIADNGDIMPPKSTWFEPKLRDGLLTHLI"
BASE COUNT         1041 a         1128 c         1120 g         1143 t
ORIGIN      
        1 gggggatgga caacaacatg ccgaaggagg gcagcggtat cccgatcttc gtcagctcgg
       61 caaagaaatc attcttgaaa agaagatagg gaaatagcag catgatcatg ttccccagta
      121 tcccggagaa atttgaagcg agggtattat tcctggcatc atcatcggag aattcattgg
      181 tgagcaatgg ccatgtgatc aggccggttg caaaggaact tgtcagactt gacagggtga
      241 actgcacgaa ggcaatgtaa atggccgtgc cgtgatcccg gtaaggattg cgccagaaca
      301 gaataaggat gtagacgata aagcctatat tgatgagagc attcaaaaag acgatcagca
      361 aaaccagttt gctatgctgt gcgccgctga tagtagaaag gaaccggatc ttttcctttt
      421 tctccaacaa cgtaaatcct aacaccgcgc cggctacgga ataaatggcc tgcgcgagaa
      481 tgctggccgc cagcagcagg agtatggcgg ccatggggaa aatacccgtc aggacaccat
      541 aggagaggaa cgtgatcagc aggccgaggg catagtagaa aaaagtataa aagacggatg
      601 gttttttgcc tgaatagaac cgggcattat tccgccggct tcgcgttgaa atgaatatgc
      661 cggcgactat cagcacgaga taggcgatgg tcagaagaga tttcatgaag aaggttttgg
      721 gttatctgtc tgaaatatcg aaagagatcg atagggggag ggatgaagcc ggaataaata
      781 tagttttatt ttcgtatatt taaagacagt tctttttttc ttacacaaaa aaacgcagct
      841 atatgaacct cgtacaacgc gtggaacgct ggggtgatac tcaccaccct ttatggctcg
      901 acttcgttcg cattctgctc ggagccttcc tttgctacaa aggcgtcgat tttctcatga
      961 acatgggtca gatgttgaac ctcatcacga acaggatgtc cttcggtacc ttcgcggcga
     1021 tgctcatgag caattatatc tccttcgcac atttgctggg agggatcctg ctcattttgg
     1081 gtgtactgac gcggttcgcc tgtcttgtca atatacccat tcttctcggt gctgtctttt
     1141 ttgtaaatac gacgctctac cggccatttt ccgaagtgtt ggtttcgatc cttatcctgc
     1201 tgctgctgat cctgttcctg atcgtcggca acggcaagat gcagatgctc acattcaggg
     1261 aagagaaaca atagatcagg actccctgta gatcttttcg atggcggcat ggaatttctc
     1321 cgtcaccacc ttgcgccgaa ggcttagctt cggcgtcatt tcccccgtat cgatggtcca
     1381 ttccgcaggc aacagctcaa agcgtttgat ctgttccaca tggttgaaat tggtattgta
     1441 gctgtccacg atctcgcggt aaaaggccag cactttggga ttacggatca gttcttcatg
     1501 cgtgccgtcg gctatctcat tttgtttggc ccacgctttc aggttcgcaa acgccggaac
     1561 tataagggcg cctacgaatt tcctttccgg cccgaccacc atgatctgct ctacgaacgg
     1621 agactctttc aatttggtct cgatcggcaa tggggcgaca tacttgcctc cgctggtttt
     1681 gaacatttcc ttcttccggt cggtgatctt cagaaaacgt ccttcgacga gcacgcctat
     1741 atcacccgtg tggaaccatc cgtccgtaat agcctctgtc gtaagatcgg gacgtttgta
     1801 ataacccatc atgacattcg gccccttgca gaggatctcg ccgtcttcgg caatgttcac
     1861 ctctataccg ggaatgatcg gaccgacggt accgaatagc cgatccttta tctccatccg
     1921 gttgacggcg atgaccggag aagtctcggt caaaccgtat ccttccagga tgggtatccg
     1981 cgccgcggtg aaaatgcgga tcagtctcac ctgtgcggcg gcgcctccgc ttacgatggc
     2041 cacgatattg ttcccaagcg cctctctcca tttgctgaag atgagcttat tggccaacgc
     2101 caattgcagg tcgtacgcga atcccatgga cttgttgatc tcataccttg ccgccagccc
     2161 atgcgcccag aagaacagct tcttcttgac accagtgagc tcggcgccct tgtgcatgat
     2221 cctgtcatat actttttcga gcagcctggg cacggtaacg aacatctgcg gtttcacctc
     2281 tttcaggttg tccccgatag tgtccatact ctcggcataa tacaccgaca cagcgctgaa
     2341 cagatacaga taggaaagca tccgttcata gatgtggttc aacggcaaaa agctgagggc
     2401 ccttcgggtg ccttgcggca aacagggaat ggcagcctgt acattggaga ggatgttgcg
     2461 atgcgaaagc atgacgcctt taggcgtccc ggtggtgccg gaggtatata tgacggttgc
     2521 cagctcgttc tcgccgatcc cgtttcttat cgaatcgagt cttttggttt cttccgccgt
     2581 cggctctgcc agcaattcct tccagtgacg gacatttttt acgttttcga aggtgaatat
     2641 ctcccgaaga ctggccactt tgtcacggat ggacgatact ttgtggaaga gctccccgtc
     2701 gttgacgaag atgtatttca cctgcgcatc attgaggatg aactccagtt ccgaaggtga
     2761 gatcgtggga taaatcgggg cgagaacggc gccggtccgc tggaccgcca tatccagcat
     2821 gatccattcc ggccggtttt tggaaatgac ggccacctta tcgatgcctt ccacgctacg
     2881 gtcgccaccc ggaatgccca gtctcaacaa gccggtgctc aattgccgga ccagtgtcgc
     2941 gatatccgtc gtgctatagg tcttccaggt tccgttctcc ctggcagcaa gcatgtcggg
     3001 aagagggtcc ttttcaaggt gcagctccag gcaatcgaat aaccgtttag gctccatcat
     3061 gacatcagtt tataagatca aattagggaa aaaaatctgg ccggcctact tcgctgcatt
     3121 ctgataatat tgtccctcgg gtaacttaaa tatcctccct ttctgaaaat tgataaagcg
     3181 attatacacg tcgctatgcg tgtttgtcca cgcctgaaaa gcgggaagat tacccgcggc
     3241 gccgaagatc cactcattat aagcctcgaa catcccctct ttcagcagct gccgctgata
     3301 atcgaaaaga cgaaacgggt aattcgatgg gtaagtattg aaccaattga taatgaactt
     3361 agtacgaagg acagtcaggg attctgcagt gatcccgtcc cggaccgtaa aattcagcgg
     3421 tgccatgacg ttgagccatg ccatcccgaa ggaattctta gtatcctggt cttttttcat
     3481 gtccggatcg agaaatagct tcttataccc ggccagcaac aggtccttca tctccgccgt
     3541 tcgtttgcta tagctttcga gattgatgaa gatctctcca tacagcaagg cccagacctt
     3601 gtcccttgtg aaatagtaat atttggcggc attgtaatag ttggaagaat aattggggtc
     3661 ggtttctatc cctttctccc attgccggat agccgaatag tcctggctgg cccagagcac
     3721 ttctccatat tcattgtaca atacgccgct gcgcgggaaa cgcttaagcc cggcgcggta
     3781 cagcttttcg cattcctttc ggtcgtcttg caccttatag aacataccca cgatctggta
     3841 cgtctgcaca tcggcatcgg gacggtcgat caatggtttg gcgatactca gtcctttggt
     3901 ataatccctg ctcagatagt agttgaaaga gaggtccttg gataattcga ggttccgggg
     3961 atccttttga agagcgccgt tgagcactac gatggcgttg gtatagtcgc cctgctgagt
     4021 ataggtgcgg gctacttcct gcggcgtttt tccgtcggat gcctgcgcct gcgcctgccc
     4081 gaccacggca aaagtcaaca aggatacagc caagaaaagg gtcctgctca tgcttttcgc
     4141 gttttgacgc taaagataat gcattatatc agatgcgtca gcaacccgtc ccgcagcttg
     4201 ggttcgaacc acgtgctctt tggcggcatg atatccccgt tatcggcgat atcgaaaagt
     4261 tgttcaatgc tgacaggata aagactgaaa gccgccttca tctcgccgct gtctacgcgt
     4321 cgctgcagtt cgcctaaacc gcggatgccg cccacgaaat tgatccgttt gtcggtacga
     4381 gggtccttga tggccagcag tttgtcgagc acgttttttt ggaggatcgt ca
//
LOCUS       DASYSV010000010         9856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2178944, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000010 DASYSV010000000
VERSION     DASYSV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2178944"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VGQ51_00290"
     CDS             complement(<1..742)
                     /locus_tag="VGQ51_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV9035015.1"
                     /translation="MISFAGKYDVLLGLIIVTLVLSAYIILYNLSRRKRAEKDLRESE
                     SRFDLLVQHVRDYAIYMIDPQGLVMSWNQGAQFIKGYTAEEVIGKHFSLFYTDEEIQR
                     GEPAFNLKKAAGDGHYECIGLRKRKDGSSFYADVVFTAMYDDKGQLSGFIKITKDITQ
                     QITIQEEMRRALDREKELNEMKSKFVTLASHEFKTPLSVILSSTSLIEKYAATGEESH
                     RLRHIQRIKSNVNNLKQLLNDFLSLEKLE"
     gene            1135..3189
                     /locus_tag="VGQ51_00295"
     CDS             1135..3189
                     /locus_tag="VGQ51_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEV9035016.1"
                     /translation="MCYSLPTTHLLFSAMIGKAFFSFHSVAGWTLILLFLIFILLVAT
                     LYLYFRTTEIRALAPGRKAEMDKRQLAEYISTLDKQQIDRYLENAKPSSGCKEPGVTR
                     LAFAPLLLGITQGSIWGETGVLIMLVLLLLPVLAGIVVMIFKVGNILRREREKQRLAE
                     AKRFSEGLSILSPAERQALLTERRDALGYGLTQRELIGELPPEDKKGLLSNVTSQAAL
                     TVVPLKKRAVKRPPIDPALARLVLAYIGCATFWLVVGTTVGEYLGIKFVAPDADHVSW
                     LSFGRLRPVHTNMVFWGWASLGMLGLGYYIVPRVGNNPLASLRKGWYSLWLINAAVLF
                     GSLCLMGGINNAGGEYREYIWPVMALFAIALMITLINFLQTIGRRKPKEIYISNWYIV
                     AAIIFGLVIVTVGYLPFWQDGLGETIVQGYYMHQAVGMWFMMFTLGIVYYFLPQQLNR
                     PIYSYSLGILAFWTQILFYTVIGTHHFIFSSIPWWLQTFAIVGSVGMIIPVAAGTTNF
                     ILTFKGGWHKLSSSYTLPFFLVGVVFYFTGSMQGTAEAFRSTNLFWHFTDFTVAHSHL
                     TMYGIISFFLWAGMYAVVPRLTGREPPQILVGTHFWMALIGLLFYTVPLMYGGTLKGL
                     LWIAGKPFIEGVTLMEPFWLWRAIGGSLMWLSHFFFAYNLYKMTTATRIKQKDYGILQ
                     QP"
     gene            3167..4186
                     /locus_tag="VGQ51_00300"
     CDS             3167..4186
                     /locus_tag="VGQ51_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HEV9035017.1"
                     /translation="MEFFNNHKKLFRAVFLFFGGLTLLVAIMPALDNQRNFRPLPDAE
                     PLSDAAVKGKMIFIANGCVACHTQQVRNVDMDKTWGSRPSIAADYAGDHRIDVWRNTA
                     TLMGTERTGPDLTNVGLRQPSAAWNLTHLYNPRILVKESVMPAYTWLFTTTMNPNPED
                     VVVNVPAEFRDDAGDGKVVATPEALQLLAYLQSLKQTKLPDGRPVPDFLFKKKDKAES
                     GGSGKTTAFDGAALYATNCQSCHQPNGEGLKGAFPPLKGSLVVLNDNPEIMVNIIMNG
                     YSGRIKEGYGPMPAVGTNNNLSPEEITAIMNHEKTSWGNNARAVSPDEIKKLVDLIKL
                     QGTTK"
     gene            4193..4456
                     /locus_tag="VGQ51_00305"
     CDS             4193..4456
                     /locus_tag="VGQ51_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035018.1"
                     /translation="MKTSYLLLLLSLDPITGILACPACTLQQPRLLRGITHGVGPDSR
                     WDYLIVSVAVAIVLGSLYFSIKWLIRPGERSETHIKQVILTFD"
     gene            4458..4871
                     /locus_tag="VGQ51_00310"
     CDS             4458..4871
                     /locus_tag="VGQ51_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEV9035019.1"
                     /translation="MVDNSTIYLFLDEDPKPIAELVSPVNFELDTTRLIDGRHILKIV
                     SKDPSGKEGLRLIPFEVRNGPSITIEGLKEEAVVDGVVPIMINAYGKGNQKEFLITGS
                     ETPQSIPFWIFILVIGFIGWAVYYFISNSNVNLFK"
     gene            4868..5266
                     /locus_tag="VGQ51_00315"
     CDS             4868..5266
                     /locus_tag="VGQ51_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEV9035020.1"
                     /translation="MIKGSDHTTNRDIQTEDDIVDLVNSFYEKVRRDDLLAPVFEATI
                     KGDWDQHLQRMCDFWSTLLLYSQKYRGDPMTRHMALNIGREHFSRWLQLFTRTVDNLF
                     KGENAKEAKKRAGYIAKLMQLTKNPSITDS"
     gene            5288..5719
                     /locus_tag="VGQ51_00320"
     CDS             5288..5719
                     /locus_tag="VGQ51_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035021.1"
                     /translation="MLSISIPLIWCGMIAAISFIEAPLKFQAPGVTLPIGLGIGKLVF
                     HALNRVECVFFICWIITSFIGKTTYYDLITPLLIGAILWAETSWLLPHLDFRATQIRE
                     GIQPSGRSLHLYYAAGEVIKFILLAGSGLIQLFKIIKNFSI"
     gene            5716..6675
                     /gene="ric"
                     /locus_tag="VGQ51_00325"
     CDS             5716..6675
                     /gene="ric"
                     /locus_tag="VGQ51_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HEV9035022.1"
                     /translation="MSTIEILDVTKIEPKKKHPVIFEHFDVLEQGEAFIISNDHDPKP
                     LYYQLLGERGSIFTWDYLEEGPALWRVKIGKRTTREGVETIGQIAAKDIRKADVFRRM
                     GIDFCCGGKQTLKEACEQAGVSEEHLQFALKEAVMSSPSVSQDFTRWDLDFLIDYIIQ
                     THHKYLMENARIISDLGQKVAQRHGKYHPELKKLVEGLSGFLRDMMTHIMKEEMILFP
                     AIRQMVAKERDPKLETGLTAGLVREAIMMMQTEHEISGEDLKFFRKITHDYALPEDAC
                     NSYTYLFEKIKEFESDLLQHIHLENNILFPAAVKLESKFSAHL"
     gene            6790..7533
                     /locus_tag="VGQ51_00330"
     CDS             6790..7533
                     /locus_tag="VGQ51_00330"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEV9035023.1"
                     /translation="MRKLLAARITLSEAAEMGGCSVGDVVRALQPLGFVFEGADTPAM
                     AVEMAEEPQWIFEKAGGKSVDLDVRPMLRDGVDPLKEIVQKFRELEHRGVLCIVNSFI
                     PAPLIRLLENMGAESFTKTGHCGEIRTYFLKTGGAQEKLKAIRETPEGRNKKLFKDDS
                     RTFAEIATRYPADRTHAINVKDLEMPLPMNAILRSLKQLPQDHALYVYHKRTPVYLLE
                     ELMDEDYEIHILTISDTDTRLLLFKKQQD"
     gene            7533..8792
                     /locus_tag="VGQ51_00335"
     CDS             7533..8792
                     /locus_tag="VGQ51_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035024.1"
                     /translation="MAGINIGKAPKNNAVLPFYGTGAVFFLALTLMILFCPAAVSGHY
                     FQPHLLAIVHTAALGWGTMVIFGASYQLLPVICEKELYSAWIALLSYFCLAVGAIFLI
                     LSFWLFRTGVIMIGGGSLILIAALLYLLNAWKTAGSCKKYSVQRCFIISSAIWLVVTA
                     SVGLVLAVNLRYPFIRGSHLEILKLHAHAGLAGWFLQLIAGVSTKLVPMFLLGKSDKE
                     KLLRWAFLLQNTGLIAFILDGYFNGITGRVFFYEGMVLAGVIAWLVYLTDICRNRVKK
                     KIDFQMKYVGISILALLISFLLIPAIFYGGDHHFAIVYGVFVFLGWISAIILGMTFKT
                     LPFIVWNERYKNINGKVKIPMPKHLYKENVLNWQFLCYIMALPLLALGIFMHIGTIVR
                     LSGIIWVMVAALYTYNVAVVIFHKRRV"
     gene            8842..9120
                     /locus_tag="VGQ51_00340"
     CDS             8842..9120
                     /locus_tag="VGQ51_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV9035025.1"
                     /translation="MNALYRVVDPELFVNIVDLGLVYGLDFAQEKSIEVTMTLSTPYC
                     PMGEAITTGVRNVLSVEYPTKEINVRIVWEPKWDHTRISDEGRRQLGI"
     gene            complement(9182..>9856)
                     /locus_tag="VGQ51_00345"
     CDS             complement(9182..>9856)
                     /locus_tag="VGQ51_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HEV9035026.1"
                     /translation="EKHLFAGAKTVVLSGPTKSKNTPTVIHGVNTEDGKVAIFSCASC
                     TTNNIGPIMEIIDRRIGIKKAILNTIHAYTASQSLLDAPSKRDLRMGRAAAMNLVPSS
                     TGAALATTKALPGLEGKFDGVSIRTPVAVGSISDITLVAARATSAKEINSILIEEANT
                     ERYRSVIATSDKPLVSSDIVQSPFAAIIDLQMTRVVENDLVKIMAWYDNEWGFTNQMI
                     RQILEL"
BASE COUNT         2497 a         2295 c         2511 g         2553 t
ORIGIN      
        1 cttccaattt ctccagggag agaaaatcgt ttaataattg cttgagattg tttacgttgg
       61 atttgatgcg ttggatatga cggagtcgat gcgattcttc gccggtagcg gcatatttct
      121 cgataaggct ggtagaggag agaatgacgc tgagcggcgt cttgaattca tgactggcga
      181 gcgtgacgaa tttcgacttc atctcgttga gttctttttc cctatcgagg gcacgacgca
      241 tttcttcctg tatggtgatt tgttgagtga tgtccttggt gatcttgatg aaaccggata
      301 gttgcccttt gtcatcgtac atggccgtaa aaaccacgtc tgcatagaag gaagacccgt
      361 cctttcgctt gcgaagaccg atgcattcat agtggccgtc cccggctgct tttttgaggt
      421 taaaggcggg ttcaccgcgt tggatctctt catcggtata gaacagggag aagtgtttac
      481 cgatgacctc ttctgccgta tatcctttga tgaattgcgc accctggttc caactcatta
      541 ccagaccctg ggggtctatc atgtagatag catagtccct gacatgctga accaggagat
      601 cgaaccggct ctcggattcg cgaaggtctt tttcggccct tttgcggcgg ctcaaattgt
      661 aaaggataat gtatgcgctc aagacgagtg ttacgatgat caatccgagt aagacgtcgt
      721 atttgcctgc aaaagaaatc attcgaacta ttttgagagc cgtggtttcc gatggacgac
      781 catggtagaa gggataaaag cgccgccgtt cattataaag atagtgcagc cggggtttcc
      841 tttttgacct atttctgtaa ccatgctcat ttccttgcgt gagggcagtc atgtgcgacg
      901 tgagcggcgc gcgaatccga tggtatgaga cggcgagtgg tggattttta gcggatatgc
      961 caaattgaaa aaattaaagg ataaattagt cttttattta aaaatcattt ttacctttgt
     1021 gagagaatgc tcaatcatcc catgatggaa gcaaggtaag gatcaaggag attgcgaagg
     1081 gtatcttaaa tgcatgattt tttttgcctt aataaacgat aaaaaggtct ttatatgtgt
     1141 tattcattgc cgaccaccca tctgctgttt tccgcaatga tagggaaagc ctttttctct
     1201 tttcattctg ttgcgggctg gacgcttatc ctgcttttcc tgatattcat cctcctggtg
     1261 gcgacgttgt acctctattt caggacgacg gagattaggg cccttgcacc gggcaggaaa
     1321 gcggaaatgg ataaaaggca actggccgaa tatatttcca cgttagacaa acagcagatt
     1381 gatcggtatt tggaaaatgc aaaaccgtcg tcaggctgta aagagccggg tgtcactcgt
     1441 ctggcattcg caccgctatt gcttgggata acacaaggct ccatttgggg ggaaaccggc
     1501 gtcttgatca tgctggtcct tttattgctc cccgtattgg ccggcattgt ggtaatgatc
     1561 tttaaagtag gcaatattct ccgccgggaa cgggagaaac aacgacttgc ggaggccaag
     1621 cggttcagcg aaggcctaag catcctctct ccggcagaaa ggcaagccct cttaacagaa
     1681 cgccgggacg cactaggcta tggcctaacc cagcgggaac tcataggaga attaccccct
     1741 gaagacaaaa aaggactgtt gagcaatgtt acgagccaag ccgccttgac tgtcgtaccg
     1801 ttgaagaaac gggcggtgaa acggcctcct atcgacccgg ccctggcacg gctcgtactg
     1861 gcctatatcg gttgcgctac tttttggctc gttgtcggca ccaccgttgg cgaatacctt
     1921 gggatcaagt ttgtggcccc cgatgcggat catgttagct ggctgagctt tggaaggctt
     1981 cgccctgtac ataccaatat ggtgttttgg ggatgggcct ctttagggat gctgggcctg
     2041 ggctattata tcgtgccccg cgtcggcaat aaccctctgg ccagccttcg gaaggggtgg
     2101 tattcgctgt ggctgatcaa tgccgcagta cttttcggta gcctttgcct gatggggggc
     2161 atcaacaatg caggaggtga ataccgggaa tatatctggc cggtcatggc gctgttcgca
     2221 atcgccctga tgatcaccct gatcaatttc ctgcagacca tcggtcgtcg caaaccgaaa
     2281 gaaatttata tctccaactg gtacatcgtg gcggcgatca ttttcggcct cgtgatcgta
     2341 acggtggggt atcttccgtt ttggcaggac gggttgggtg aaaccattgt ccagggctat
     2401 tatatgcacc aggccgtcgg aatgtggttt atgatgttta ccctgggaat cgtttattat
     2461 ttcctgcccc aacaacttaa ccggcccatc tactcctata gccttgggat cctcgctttc
     2521 tggacacaga tactgtttta taccgtgatc ggcacccatc atttcatttt cagttctatc
     2581 ccctggtggc tccagacctt tgccatcgtg ggaagcgtcg gaatgatcat tcccgtggcg
     2641 gccggcacca ccaattttat cctgaccttt aaagggggct ggcacaagtt atcgtccagt
     2701 tatacgctgc ccttcttttt agtaggggtc gtcttttatt tcaccggctc tatgcagggt
     2761 acggcggaag cgttccgctc caccaaccta ttctggcatt tcaccgattt cacggtagcc
     2821 cactcgcatc tcacgatgta cgggatcatc tccttttttc tttgggcagg tatgtacgcg
     2881 gtcgttcccc ggctcacggg cagggaaccg ccccagatcc tcgtgggcac acatttctgg
     2941 atggcgctga ttggcctgct gttttatacc gttcccctga tgtatggagg gacgctaaag
     3001 ggactcttat ggatagccgg caagcctttt atcgagggag tgacattgat ggaacctttt
     3061 tggctatgga gagccatcgg gggtagcctg atgtggcttt cgcacttctt cttcgcctat
     3121 aacctataca aaatgacgac ggctacccgc atcaaacaaa aagactatgg aattcttcaa
     3181 caaccataaa aaattattta gggcggtatt cttattcttc ggaggattga cgctgctcgt
     3241 ggccatcatg cccgcactcg acaatcaaag gaatttcaga ccccttcctg atgccgagcc
     3301 cctgagcgat gcagccgtca aaggaaaaat gatctttatc gccaatggat gcgtggcctg
     3361 tcatacccag caggtccgga acgtggatat ggacaaaaca tggggttccc gccctagcat
     3421 tgcggccgat tatgccgggg atcaccggat cgatgtctgg cgcaataccg ccaccttgat
     3481 gggaacggaa cgtaccggtc cggatctgac caatgtcggc ttgaggcaac ccagcgcagc
     3541 atggaacctc acgcatttat acaatccccg tatcctggta aaggaatccg tcatgccggc
     3601 ctatacctgg ctgtttacca ccaccatgaa tccgaacccg gaagatgtgg tcgtcaatgt
     3661 acccgcggaa ttcagggacg acgcaggtga tggaaaggtt gtggccactc ccgaggcgct
     3721 gcaattgctg gcctacctgc agtccttaaa acaaacgaaa ctgcccgacg gcaggcctgt
     3781 accggatttt ttgtttaaga aaaaggacaa agcggaatcc ggcggcagcg gcaaaacgac
     3841 cgcctttgac ggtgcagcct tatatgcgac gaattgccag agttgccacc agcccaacgg
     3901 agaaggactt aagggggcct tcccaccatt aaagggcagc ctcgttgtac taaatgacaa
     3961 cccggagata atggtcaata tcatcatgaa tggttattcg ggccggataa aggaaggata
     4021 tggaccgatg cccgcggtgg gaaccaacaa taacctcagc cctgaagaga tcacggctat
     4081 tatgaatcat gaaaagacca gctgggggaa taatgcccgg gcagtgtctc ccgatgaaat
     4141 aaagaagctg gtggacctga tcaaactaca aggaacaaca aaataaaaac tcatgaagac
     4201 atcctatctc cttctattgt tatcactgga ccccatcacc ggtatccttg cctgccctgc
     4261 ctgtaccttg cagcaacccc ggctgttgcg cgggataacc cacggggtag ggccggacag
     4321 tcgctgggac tatctcattg taagcgtagc tgtggccatt gtactgggga gtctctattt
     4381 ttcaataaaa tggctcatcc ggccgggtga gaggtctgag acccatatca agcaagtcat
     4441 tttaacattc gattaatatg gttgacaaca gtacgatata ccttttcctg gacgaggacc
     4501 cgaagccgat cgcggaactt gtttccccgg taaattttga gctggatacg acccgcctta
     4561 tcgatggcag gcatattctg aagattgtga gtaaggaccc ctccggaaaa gaagggctgc
     4621 gcctcatccc gtttgaagta aggaatggcc cgtccatcac catcgaggga ttgaaggaag
     4681 aggcggtggt ggatggcgta gtgccgatca tgatcaatgc ctacggaaaa gggaatcaaa
     4741 aagaatttct cattaccggc agcgaaacgc cccaaagcat tcctttttgg atattcatcc
     4801 tggtaatcgg atttatcggc tgggcagttt actattttat cagcaattca aacgtgaatc
     4861 tcttcaaatg ataaaaggat cggaccatac tactaacaga gacattcaga ccgaggacga
     4921 tatcgttgac ctcgtcaata gtttttatga aaaagttcgt cgggacgacc tgcttgcacc
     4981 tgtatttgag gcaaccatta aaggagattg ggaccaacac ctgcaaagga tgtgcgattt
     5041 ttggagcacc ctgctgctgt acagccagaa atatcggggc gaccccatga ccaggcacat
     5101 ggccttgaat atcggcagag aacattttag ccggtggcta cagctcttta cacggacggt
     5161 ggataacttg tttaaaggag aaaatgccaa agaagcgaag aaacgagcgg ggtatatcgc
     5221 caagctaatg caattgacaa aaaatccttc cattacagac tcataaacca tgataactat
     5281 agccagaatg ctatcaatat cgatcccgct catatggtgc gggatgatag ccgccatcag
     5341 ttttattgaa gcgcctctta agttccaggc accgggcgta acccttccga taggactggg
     5401 tatcgggaaa ttggtttttc atgcgctaaa ccgggtcgag tgcgtctttt tcatttgctg
     5461 gatcattacc tcctttatag gcaaaaccac ttattatgac ctgatcaccc ctttgctgat
     5521 cggggctatt ctttgggccg aaacctcctg gctgctcccg cacctggact tcagggcaac
     5581 ccagatccgg gaaggcatac agccctccgg cagatccttg cacctctact atgcagccgg
     5641 cgaggtcatc aaattcatcc tgcttgccgg gagcgggctg atccagcttt tcaaaatcat
     5701 caaaaacttt tcaatatgag caccatagaa attttagacg tcacgaaaat agagcctaaa
     5761 aaaaagcacc cggttatctt cgaacatttc gacgtcctgg aacaggggga agcctttatc
     5821 atttcaaacg accacgatcc caaaccgctg tattatcagc tacttggcga acgcgggagt
     5881 atatttacct gggattacct ggaagaaggt ccggcgctat ggagggtaaa aatcggaaaa
     5941 aggacgaccc gcgagggggt ggaaactatc gggcaaatag ccgccaaaga tatcagaaag
     6001 gccgacgttt tcaggcgaat gggtattgat ttttgctgcg ggggtaaaca gacactgaaa
     6061 gaagcgtgcg aacaggccgg agtctccgaa gagcacttac agtttgccct gaaagaagcg
     6121 gttatgtcct ctccgtcggt ttcgcaggat tttaccagat gggatctcga ttttctaatt
     6181 gattatatta tccagacaca tcataagtac cttatggaaa acgcacgtat catcagcgat
     6241 ctgggacaga aagtggcgca aaggcatggt aagtatcatc cggaattgaa aaaactcgtg
     6301 gaaggcctgt ccggatttct ccgggacatg atgacccaca taatgaagga ggaaatgata
     6361 ttgttccccg ccatcaggca aatggtggca aaggaacggg atcccaaact ggagacaggt
     6421 cttacagccg gcctggtaag ggaggccata atgatgatgc aaacggagca cgaaatatcc
     6481 ggcgaggacc tgaagttctt caggaaaatc acccacgatt atgcgcttcc cgaagacgcg
     6541 tgtaactcct atacctatct tttcgaaaag ataaaggaat tcgaaagcga tctgttgcag
     6601 catatccacc tggaaaataa tatccttttt ccagcggcag taaaactgga gagcaaattt
     6661 tccgcacatc tttagactat gatcatcaac gggcaaacga aaatatccag cataataaag
     6721 gcgaacaagg acagtattga agccattgcc tcggtggcca ggccattgga gaaattgaaa
     6781 aacccgcttt tgcggaaatt gctggcggca aggatcaccc tgtcggaggc agcggaaatg
     6841 ggcggatgct ctgtcggaga tgtcgtccgg gcattgcaac cgctgggatt tgtctttgaa
     6901 ggggcagata caccggcgat ggcggtagag atggctgaag agcctcaatg gattttcgag
     6961 aaggcaggag ggaagtccgt cgatctggat gtccggccga tgttgagaga tggggttgac
     7021 ccgctgaagg aaatagtaca gaaattcagg gagttggagc accgaggggt cttgtgcatt
     7081 gtgaactctt ttattcctgc tccgttgatc aggctcttgg aaaatatggg cgcggagagc
     7141 ttcacgaaaa ccggtcactg tggagagatc cgcacctatt ttttaaaaac tggcggagca
     7201 caggagaaat tgaaggcaat ccgggaaacg ccggaggggc gtaataaaaa actattcaaa
     7261 gatgattcac ggacattcgc ggagatagca acaagatacc ccgcggaccg aacacacgcc
     7321 atcaatgtga aggatttgga aatgcccctt ccgatgaatg ccattttgag atcgctaaaa
     7381 caacttcccc aagaccatgc cttatatgtc taccacaaac ggacgccggt ctacctgctg
     7441 gaggaattaa tggatgagga ctacgaaatt catatcttga ccatttcgga tacggacaca
     7501 cggttgctgt tatttaaaaa acaacaagac taatggcagg cattaacatt ggaaaagcgc
     7561 ccaaaaataa tgcggtcctg cctttttatg ggacaggagc ggtctttttc ctcgcgctaa
     7621 ccctcatgat tctcttttgc cccgcagccg tgtcaggtca ttattttcag cctcatttgc
     7681 ttgcaatcgt tcataccgcg gcgcttggat ggggtactat ggtcatattt ggagcctctt
     7741 atcaattatt gccggttatt tgtgaaaagg agctttacag tgcgtggatc gcgctgctct
     7801 cttatttctg ccttgctgtt ggagccatct ttttgatcct ttccttttgg ctttttcgca
     7861 caggtgtgat catgattggc ggcggatcgc tgattttgat agctgccctg ttgtacctgc
     7921 tgaatgcatg gaagacggcc ggttcctgta aaaaatacag tgtacagaga tgttttatta
     7981 taagctccgc tatttggctg gtagtgacag cttccgttgg cctggtcctg gccgtcaacc
     8041 tgcgctatcc gttcatccgg gggagtcacc tggaaatcct aaagctgcat gcccatgcgg
     8101 ggcttgcggg ttggttcctg caattgatcg caggcgttag caccaaattg gtgccgatgt
     8161 tccttttggg aaaatctgac aaggaaaagt tgcttcgatg ggcctttttg ctgcaaaata
     8221 caggacttat cgcgtttatc ctggatggct atttcaatgg aatcacaggg agagtatttt
     8281 tctatgaagg gatggttctt gctggtgtga tcgcctggct ggtctatttg accgatatct
     8341 gccggaatag ggtaaagaag aaaatagatt tccagatgaa atatgtcggt atttcgatcc
     8401 tggcactgtt gatcagtttt ctgctgatcc ccgcgatctt ctatggtgga gatcaccatt
     8461 ttgcgattgt ctatggtgta tttgtatttc ttggttggat cagcgctatc atattaggca
     8521 tgacgtttaa aacgctaccc tttattgtct ggaatgaacg gtacaaaaat atcaacggca
     8581 aagtgaagat ccctatgccc aaacacttgt ataaagaaaa tgttctgaat tggcaatttc
     8641 tctgttatat catggctttg ccgttactgg ctttaggaat ctttatgcac atcgggacaa
     8701 tcgttagatt atcgggtatc atttgggtga tggttgccgc gctatacacg tataacgtgg
     8761 cggtggtgat ctttcataaa aggagggtat aagatgggaa ttttaataac cgatccgttt
     8821 tatgaggaaa aacaaatagc aatgaatgca ctataccggg tcgtcgatcc ggagctattt
     8881 gtgaatatcg ttgacctggg attggtctat gggctcgatt ttgcccagga aaaatcaatc
     8941 gaagtgacga tgaccctttc gacgccttat tgcccgatgg gggaagcgat aacgacgggc
     9001 gtacgcaatg tgctttccgt cgaatacccc actaaagaga tcaacgtaag gatagtgtgg
     9061 gaaccgaaat gggatcatac gaggataagc gacgagggca gaaggcagtt gggaatatag
     9121 atcctatcct gaaagcacgc tcacaccaat ccttttattc cccggtaagt tctggaaaaa
     9181 cctacagctc caggatctga cggatcattt gattggtaaa tccccactcg ttatcatacc
     9241 atgccattat tttcaccaga tcattttcca cgaccctggt catttgcaga tcaattattg
     9301 ctgcaaaagg gctttggaca atatccgagg agaccagggg cttgtcggaa gttgctatta
     9361 ccgacctata tcgctccgta tttgcctctt caatcaggat actattaatt tcctttgccg
     9421 aagtggctct tgcagccaca agcgtaatat ctgaaataga acccacggcc accggagtgc
     9481 gtatggatac cccatcgaat ttaccttcca atccgggcag agccttcgtt gtagcaaggg
     9541 ccgcgccagt cgacgagggt acgaggttca ttgctgcggc acgtcccatt cgcaggtctc
     9601 ttttggaggg agcgtcaaga agggactggg aagccgtata ggcatgaatg gtatttaaaa
     9661 tggccttttt tataccaatc cgcctgtcga taatttccat gatcggcccg atattgtttg
     9721 ttgtacagct ggcacatgag aatatagcta cttttccatc ctcggtattt accccatgta
     9781 tgacagtagg cgtgtttttg cttttggttg gaccggatag cacaacggtc ttcgctcctg
     9841 caaagagatg cttttc
//
LOCUS       DASYSV010000011         6561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_771175, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000011 DASYSV010000000
VERSION     DASYSV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771175"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VGQ51_00350"
     CDS             complement(<1..359)
                     /locus_tag="VGQ51_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035027.1"
                     /translation="MRILFINYVDISGGSAVVLQRLKKGLARYYGVEGHLLVKIRSGD
                     APDTEQLLIRPFPKLVEKAIDRVSRRAGLLYQYFPFTSQKILDSAADYRPDIIHLHTT
                     QGGYFATPLLRRLSGIAP"
     gene            complement(359..1333)
                     /locus_tag="VGQ51_00355"
     CDS             complement(359..1333)
                     /locus_tag="VGQ51_00355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV9035028.1"
                     /translation="MVKSLFGKGRRYFFNLVDTPAVILLYHRVTRLEHDPQQLSVDPD
                     NFYDQVQLLKRDYALLNIGEFTDLVSRRRRLPKRSVILTFDDGYADNLLEAVPILANL
                     DRQALFYITTSNIGTSYELWWDQLERIFLMNAVLPPVLEIRDHDRQLRLQTASAEERL
                     ASYYQCHWFLKYTRPDQRNEIIDNLLAKAALGKEGRATHRLMTWDELKDMGHSTAAVV
                     GAHTHHHPVLSLLSYPEQAEEIKTSTDILEKVLHRKIEHFSYPYGMRKDMNADSIRIC
                     RESGFRIVCSNFYGQVHSWSNPMRLPRILVRNWEKQEFGRQLSKFFSY"
     gene            complement(1335..2429)
                     /gene="wecB"
                     /locus_tag="VGQ51_00360"
     CDS             complement(1335..2429)
                     /gene="wecB"
                     /locus_tag="VGQ51_00360"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEV9035029.1"
                     /translation="MKKIISVVGARPNFMKVAPLHHEFCKYRDQVRHLICHTGQHYDE
                     KMSKVFFDELGLPKPDFYLGIGSGSHAVQTAGVMVEFEKIVLQEKPDLVLVVGDVNST
                     IACSLVAKKMNVRVAHVEAGLRSNDRTMPEEINRVLTDSISDFLFVSEESGMVNLRRE
                     GKTEDQLFMVGNVMIDSLINNLPKIEASGALSAFGVQKNGYFLVTLHRPSNVDDKATL
                     SKIISILNKYARSHKIIFPVHPRTRTNMAGFGLSGELSPEIRLCDPIGYLDFIKLLKN
                     ATLILTDSGGIQEESTFLGVPCITMRENTERPVTIEMGTNHLIGTDVGKIENAITGIL
                     RDGGKTGAIPPFWDGKTAERVCSTLVKILG"
     gene            complement(2449..3651)
                     /locus_tag="VGQ51_00365"
     CDS             complement(2449..3651)
                     /locus_tag="VGQ51_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV9035030.1"
                     /translation="MKVAFISPWFSEGMGYSENMFPKALARLGVDVHLVTSTAQINYY
                     LPHYDKTYKSFLGPKIVDPVVKELDGYTLHRLPYYDTKKIYEGPGITGLYEYLEKLGP
                     DVLQTFEIKLETSYESARYAQDHDRRFFTECHMHASVLWPPPRKRWKEQLKNWRNAFN
                     GRLRYINKVSRLCYPIAEDVVEIAVDHYRVPGNKIKMQSLGVDTYAFTPASSPTESES
                     REKIRREFGFSADDIVCIYTGRFTADKNPQCLAMAIDHLNAQGLPFKGLFIGNGPEKD
                     VEFIREKRGCRVGAFVPARDLPIYYRASDIGVWPREESTSQLDAAACGLPLILSDRIK
                     VTERVNGNGLLYAEGDHLDLAEKLIRLQDAGKRKSMSAHGVQKVRANFSWEKIARDRL
                     ADYLEFLK"
     gene            complement(3656..4357)
                     /locus_tag="VGQ51_00370"
     CDS             complement(3656..4357)
                     /locus_tag="VGQ51_00370"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV9035031.1"
                     /translation="MNIRRLKTGIAHLLRRSSTPVKWHSLRKTKPVSTVFGLDRGTPV
                     DRYYIEKFLSSNRQLIRGSVLEIAENVYTSKFGGSQVTRSEILHFTDDNPSATIVGDL
                     TDPATLPGDRIDCFICTQTFNFIYDFKKAIEGAHHLLKNDGVLLATLGGISQISRYDM
                     DRWGDYWRFTVLSALRAFEEVFGKGNVQTASYGNVLSSISFLEGISVEELTAAELDHE
                     DPDYQMLITVIARKK"
     gene            complement(4359..5060)
                     /locus_tag="VGQ51_00375"
     CDS             complement(4359..5060)
                     /locus_tag="VGQ51_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035032.1"
                     /translation="MVLKDLFRHPDARLPVYSLKYYADWKQYLKTGRNSVDDRMPWIS
                     FSAIAHIGRIVRPDMRVFEYGSGGSTLFWASRVQELVSVEHDRPWFERIREVLGREKI
                     ANVRYILAEPEPDTGFDRKDIADPGGYISGDEHFAGMNFERYARTIDEYPGDYFDIII
                     VDGRARPSCIAHARGKLKEGGWLIVDNTERAYYQHAIRADAKRWKKKVFGGPVPYVYH
                     FSDTTFLKKMDKHKP"
     gene            complement(5063..6418)
                     /locus_tag="VGQ51_00380"
     CDS             complement(5063..6418)
                     /locus_tag="VGQ51_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV9035033.1"
                     /translation="MNVARSVRERIAVYRESVLVKRLFAVLSIDILVKISGMLLLPVY
                     LRLMTQDDYGLYNYLISIILTFSLVLNFGLYIPLSKYYHDHQGQRDRGELLFTLFAML
                     IIFLAVVLVPVYLFHWDYAVVNILFKNKMPYDDYRNSILLAIVASVCNFMLANFFYTS
                     ERIGHLQRYNICRIIAINVLAVSFLIFFKTRGDSARIRLECTYAVEMVLFLFFCFSYV
                     REVQVRFNRKMAVASLRLATPVLVSAIFGIVLNFSDKFFLEKYGSFRDLSYYFLAVSC
                     ASLIPLIFTSFQNAWLPLFLKEKDLRKNIGKTNRLLLRLFFIFAGLSVFILIFLKVVL
                     MLGIIQTKYNESMYILPILLASQIAAALVPLYSNYLIYFEKTHIASIMGLILCALSFS
                     LSILLIPRFGVYGAALASLICNICYLAGYFFVIRNYIGHHKTTTWELQKDHTSIDHSS
                     I"
BASE COUNT         1580 a         1731 c         1666 g         1584 t
ORIGIN      
        1 ggcgcgatcc cggacaggcg gcgaagcaaa ggcgtggcaa agtagccgcc ctgcgtggta
       61 tggagatgaa tgatatccgg acggtaatca gccgcgctgt cgagtatctt ttgcgaggtg
      121 aatgggaaat actggtataa cagcccggcc cggcggctta ccctgtcgat cgccttctcg
      181 accagtttgg gaaatggacg gatcagcagc tgttccgtat ccggggcatc gccgctcctg
      241 atctttacca gtaaatggcc ttccacaccg taataccggg caagaccttt cttcagccgt
      301 tgcaggacga ccgcagagcc accactgata tccacataat tgatgaacag tatccgcatc
      361 agtagctgaa gaattttgat agttgtcgtc cgaattcctg tttctcccag tttctcacca
      421 ggatccttgg caatcgcatc gggttcgacc agctatgcac ctggccgtaa aaattcgagc
      481 agacgatcct gaatcccgat tccctgcaaa tacggatgct gtcggcattc atgtcctttc
      541 tcattccata aggataggaa aagtgctcta tcttccggtg cagcaccttt tcgaggatat
      601 ccgtggacgt cttgatctcc tccgcctgtt ccgggtagga caacagtgat agtacaggat
      661 gatgatgagt atgggctcct acaaccgcag cggtcgaatg acccatgtct ttcaattcat
      721 cccatgtcat cagccggtgc gtcgctctgc cttcttttcc cagagcggcc ttagccagca
      781 agttatcgat aatttcattc ctttggtccg gacgggtata tttcaggaac caatgacact
      841 ggtaatagga ggccagcctc tcctctgcgc ttgccgtctg cagacgcagc tgccggtcgt
      901 ggtcccggat ctccaacacc ggtggaagta cggcattcat cagaaagatc ctttccaatt
      961 gatcccacca cagctcataa gatgtaccga tattcgacgt tgtaatatag aaaagcgcct
     1021 ggcgatccag gttagcgagt atcggcacgg cctccagcag gttgtcggca taaccgtcgt
     1081 caaaggtcag gatcaccgac cttttaggca gccgtctgcg acggctcacc agatcggtaa
     1141 attcgccaat gttcagcaaa gcatagtcgc gtttcagcaa ttgtacctgg tcgtaaaaat
     1201 tatccggatc caccgacaac tgttgcggat catgttccag ccgggtgacc cggtgataca
     1261 aaaggatcac cgccggtgtg tccaccaggt tgaaaaaata acgtcgcccc ttgccgaaca
     1321 ggcttttaac catactagcc aaggattttt acaagtgtgg aacaaacccg ctctgccgtc
     1381 ttcccatccc aaaaaggagg gatcgcaccg gtctttcccc catcccggag aatacccgtg
     1441 atggcgtttt cgatcttccc gacatcggtg cctatcaggt gattggtgcc catttctatc
     1501 gtgaccggcc gctcggtatt ttcgcgcatg gtgatacacg gtacacccaa aaaggtactt
     1561 tcttcctgta tcccgccgct atccgtcagg ataagggtgg cattcttcag taatttgata
     1621 aaatccaaat agccgatggg atcgcacaac cggatctccg gagacaattc gcctgataga
     1681 ccgaaaccag ccatattcgt cctcgtgcgc gggtgtaccg ggaatatgat cttatgggag
     1741 cgcgcatatt tattgaggat ggaaatgatc ttcgagaggg tcgccttgtc gtcgacattc
     1801 gacggccggt ggagagttac caggaaataa ccgttctttt ggaccccgaa ggcggaaagc
     1861 gcgccggaag cttcgatctt gggcaagttg ttgatcagac tgtcgatcat gacgttcccg
     1921 accatgaaca gctgatcctc cgtcttgcct tcccgccgga gatttaccat gccgctctct
     1981 tcgctgacga aaaggaaatc cgagatcgag tccgtcagga cccggtttat ctcttcaggc
     2041 atggtccggt cattactcct caaaccggct tcgacatggg ctacgcgtac attcattttc
     2101 tttgctacga ggctgcaggc gatggtagaa tttacgtcgc cgacgacaag cacgagatcg
     2161 ggcttttcct gaaggactat cttttcgaat tcgaccatca caccggctgt ctgtacggca
     2221 tgggaccccg agccgatacc caggtaaaaa tccggcttcg gcaatcccag ctcgtcaaaa
     2281 aagaccttgg acattttttc gtcataatgt tggccggtat gacagatgag gtggcggacc
     2341 tggtcgcggt atttgcaaaa ttcgtgatgt aacggcgcca ctttcatgaa atttggcctc
     2401 gcgcccacca ccgatatgat ctttttcatt tttcagtatg ctgttttgct attttaagaa
     2461 ttcgagatag tcggccagcc ggtcgcgggc gatcttttcc caggaaaagt tagcacgtac
     2521 tttttgaacg ccatgcgccg acatcgattt tctctttccg gcatcctgca accggatgag
     2581 cttttccgcg agatcgagat gatctccctc ggcgtatagc aacccgttgc cattgacccg
     2641 ctcggtcact ttgatccgat cgctgaggat cagcggaaga ccgcatgccg cggcgtccag
     2701 ctggctggtc gactcttccc tcggccaaac tcctatatcc gaagcacggt aatatatcgg
     2761 cagatccctg gccggtacga aagcacctac ccggcagcct cttttttccc ggatgaattc
     2821 gacgtctttt tcgggaccat tcccgatgaa caggccttta aatggcagac cctgtgcatt
     2881 cagatggtcg atcgccatcg cgagacactg tggattctta tcggcggtga agcgcccggt
     2941 atagatacag acgatatcgt ctgccgaaaa gccgaattcg cggcggatct tctccctgct
     3001 ctcggattcc gtgggtgaag aggccggggt gaaggcgtag gtatccactc caagggattg
     3061 catctttatc ttatttcccg gcaccctgta gtgatcgacc gcgatctcca cgacgtcctc
     3121 ggctatcggg tagcatagcc tgctcacttt attgatataa cgcagacggc cgttgaaagc
     3181 attcctccag ttcttaagct gctctttcca gcgcttacgc ggcgggggcc agagcaccga
     3241 ggcatgcata tggcattccg taaagaaccg ccggtcgtga tcttgcgcat acctggcgga
     3301 ttcatagctc gtttccagct tgatctcgaa ggtttgcagg acatcgggtc ccaatttctc
     3361 caggtattca taaagtcccg taatgcccgg tccttcatag atcttttttg tatcgtaata
     3421 agggaggcga tgcagtgtat agccgtccag ctcttttacc acggggtcga cgatcttcgg
     3481 ccctaaaaag gatttgtatg ttttgtcgta atggggcaga taataattga tctgggcggt
     3541 cgaggtgacg agatgcacat cgacgcccaa cctggccagc gccttgggaa acatgttctc
     3601 ggagtacccc attccttcgg aaaaccatgg actgataaaa gcgactttca ttttcttatt
     3661 ttttcctggc tataacggtg atcaacattt ggtaatccgg gtcctcatgg tccagctcgg
     3721 cggcggtcag ctcctccacg ctgataccct ccaggaagga gatgctgctc aatacatttc
     3781 cataggatgc ggtctgcaca tttcctttgc cgaagacttc ttcgaatgcc cgcaatgccg
     3841 acaagacggt gaaacgccag tagtctcccc acctgtccat atcgtagcgg gatatctggg
     3901 agatgcctcc cagcgtcgcc agcaggaccc cgtcgttctt caataaatga tgagcccctt
     3961 ctatagcttt tttgaaatca tagatgaaat tgaacgtttg tgtgcagata aaacaatcta
     4021 tcctgtcccc aggcagcgtg gcaggatcgg tgagatcgcc gacgatggtt gccgaggggt
     4081 tgtcatcggt gaaatgcagg atctcggacc tggtcacctg cgaaccgccg aattttgagg
     4141 tatagacatt ctccgcgatc tcgagcacgg atccccggat cagttgccgg ttcgaagaaa
     4201 ggaacttttc gatataatag cggtcgaccg gggttcccct gtccagcccg aaaaccgtgg
     4261 aaacgggctt cgtcttgcgc agtgaatgcc atttgacggg ggtggaagat cttctcaaca
     4321 agtgagcaat tcccgtttta agtcgccgga tgttcatgct atggtttgtg tttatccatc
     4381 tttttcagga aagtggtatc ggaaaaatga tagacatacg gcaccggccc cccgaacacc
     4441 ttcttcttcc agcgctttgc gtcggcacgg atagcatgtt gataataggc acgctcggta
     4501 ttgtcgacga tcagccagcc gccttccttc aacttgcctc tcgcgtgagc gatacaggaa
     4561 ggtcgcgccc ggccatccac gatgataata tcgaaatagt cgcccggata ttcgtcgatc
     4621 gtccgcgcat atctctcaaa attcattcca gcaaaatgtt cgtcaccgga tatatatccg
     4681 cccgggtccg caatatcctt ccggtcaaaa ccggtatccg gctccggctc cgccaggata
     4741 taccggacat tggcgatctt ctcccggccg aggacctccc tgatgcgttc gaaccagggc
     4801 cggtcatgct ccactgagac aagttcctgc acccgggatg cccaaaacag cgtcgaccct
     4861 ccggaaccat attcgaacac acgcatatcc gggcgaacga tccggccgat atgcgcgatg
     4921 gcggaaaaac tgatccacgg catccggtcg tcgaccgaat ttctgcccgt cttcagatac
     4981 tgtttccagt cggcataata tttcagactg tacaccggca ggcgcgcatc cggatgacgg
     5041 aacaaatctt tcagaaccat ttttaaatag aggaatggtc tattgaagtg tggtctttct
     5101 gcaattccca tgttgtggtt ttatgatggc cgatatagtt tcggatgacg aaaaaataac
     5161 cggcaaggta gcaaatatta cagattaggg aggcgagggc ggcgccatag acgccgaaac
     5221 gcggaatgag caggatgctc agactaaagc tcagagcgca tagtatcaga cccatgatcg
     5281 aggcgatatg ggtcttttcg aaatagataa ggtaattcga atacaacgga accagggccg
     5341 cagcgatctg cgatgccagc aggatgggca ggatgtacat cgattcgttg tatttggttt
     5401 gaatgatgcc cagcatcaat actaccttca aaaagatgag aataaaaact gataaccccg
     5461 caaagatgaa gaatagccgg agcaacagcc ggttcgtctt gcctatattt ttccgaaggt
     5521 ccttttcttt gaggaacagt ggtaaccagg cgttctgaaa agaggtaaat atcagcggga
     5581 taaggcttgc gcaggagacg gcgagaaaat aataggagag gtccctgaat gaaccatact
     5641 tctcgagaaa gaatttgtcg ctgaaattga ggacgattcc gaatattgcg gacaccaata
     5701 ccggtgtggc cagcctgagg ctggcaaccg ccatcttcct gttgaacctc acctggactt
     5761 ccctgacgta agaaaagcag aagaagagaa agaggaccat ctctacggca tatgtacatt
     5821 ccaggcggat acgggcgctg tcgccccgcg tcttgaagaa gatcagaaaa ctcacggcaa
     5881 gcacattgat cgctatgatc cggcagatat tgtatcgttg aagatggcct attcgctcgc
     5941 tcgtataaaa aaagttcgcc agcatgaaat tgcagaccga agcgacgatg gccaggagaa
     6001 tgctgttccg gtaatcatcg tagggcatct tgttcttgaa aaggatattc acgaccgcgt
     6061 aatcccaatg gaaaagatag acgggcacca gcaccacggc caggaagatt atcagcatcg
     6121 caaaaagcgt gaacaacagt tcgcctctgt cgcgctgtcc ctggtgatcg tgatagtatt
     6181 tgctcagcgg gatatacagg ccgaaattca atacgagcga aaaagtcagg atgatcgaga
     6241 tgaggtaatt gtaaaggcca tagtcatcct gcgtcatgag tcgcaggtat accggcagca
     6301 ggagcatacc ggatatcttg accaatatat cgatgctgag aaccgcaaac agtcttttga
     6361 ccagaacgct ttccctgtat acggctattc tctctctgac cgatctggcg acattcatct
     6421 cgataggttc aaatttactt ttttggcgcc gccggaacga ggactaccgg cgcggcggca
     6481 gcggcgggat tcatgatccg cttcctcgcc tcataaaaga aggcgatcac aaagcagata
     6541 aatgcggctg cgacgccgaa g
//
LOCUS       DASYSV010000012         7550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1923888, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000012 DASYSV010000000
VERSION     DASYSV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923888"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VGQ51_00385"
     CDS             <1..211
                     /locus_tag="VGQ51_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEV9035034.1"
                     /translation="SFIYMRYHGPVGDYTGSYPDDRLSHDSDRIIAWLNQGKEVYVYF
                     NNTIGDALNNADRLIELVGDTVNHI"
     gene            complement(198..1289)
                     /locus_tag="VGQ51_00390"
     CDS             complement(198..1289)
                     /locus_tag="VGQ51_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEV9035035.1"
                     /translation="MKEIRTIVAAWQTIDFQTTRAALATVVRIEGSSYRRAGARMLVL
                     DNGTYLGGISGGCLEGDALRRAQKGIALNRPSVITYDTTRDDDYQIGVGLGCQGIIDV
                     LFTPLQADDPDCPLRILAGLTGIRQPSVVVTVTGTPDPELLGKTYLYTDDVSFEEILP
                     MGEPGPKVLAEIRHAQAERLSRSVVYDDLHLFIEIILPAIHVVAYGGSYDMRTLLRQI
                     RELGWDSTVVLQTARIEKTLVEAATTVLDSKEPDAALIDEYTAVLLMSHDYRTDMLNL
                     QRVLPTCVTYIGMLGPRKRADRLFEALTEAGCPLGEEDLRRIYAPAGLDIGAATPEEI
                     ALSILAEIRSHFSRRLGMSLRLRKGSIYG"
     gene            complement(1379..4630)
                     /locus_tag="VGQ51_00395"
     CDS             complement(1379..4630)
                     /locus_tag="VGQ51_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEV9035036.1"
                     /translation="MPQRPSPHLKIASDPEEPHPSPPVVAMIIPAGDASVFETFFRQL
                     PVSLGISYLIMEDHGSWGGRRADDGAESLAALLERTTLLPLRMPVQDGLLEKDCIYLL
                     PPGSAIQLVRGRLIFQGPVADGGIDYLPDLFLASVGAGCPGRCAAVLLPDMAARVLPG
                     LRVVRDEGGFTIVLDEQDPFLSPHPAYRAVDLFLTAPLAASWLGGLVEYSQPPEDHCG
                     PRTEVDLSSVYPLLLHRSGCDYARYRREDVVRRIHRRMAIRGMVCPNAYTTVLQTEEE
                     ELMHLEDELQSALSGFVIDAGLETALSVSVLPRLLDSAQRTAPLRVWLPHCTGGHQAY
                     SIAMVIMEYLEACQSNLAVRLFVTSFDNNAVVKARKGIFTNEEMHYVTRNRRKKFFIK
                     KDKDWQVVKSLREKCIFASHNLLKDSPFSYMDVVIESGSLIGFGSGDCARAFRLFHYA
                     LTPDGYFIANVLHPHGIPAELYIASQGYHGVYLKADVRVNLWQDDQGPANMADSGELE
                     ADKLLLSGYVPASFLVDEQFRVIRYYGNTEPYLRACRERSSRSLFRIVKDELVFELND
                     LIDLTDKEGRAITRKGIQLGEDSNSKEVSVEMVPLRSFGVNRKLVIIREIARRREAEP
                     SREDGSDGVSSLKGLRRQSIEKEIQEMRGLLLVADRDATRQQSGLQRANKDLQASNEE
                     FRSLNKELQELNGELNEMYGEMQTKYKELNVLNEGLRTQIREIHSVADAAMAIVASFS
                     RSIVVLRDDLRIHTVNRAFLHLFDLASHEAVGQYLTRLGPDLFRKDSIRFRLQHVLKD
                     QLGAEFELRYDTRDGKEKLLHFSATRMAGLPGLRTGILLSIDDVTEQRDLQRFKDNLI
                     GLASHELRTPATSIRAYSEMLSQELTDNGDKRSAFLVGKLNSQVDRLAGLTRDLLDVS
                     RITRGRIVIDEVPFDLNTLITEIAEATQTTSSVPIFVDPLPSSPPLQGDRERIGRVLG
                     NLLSNAAKYSDHAGQIAIRTVVTQEHVRLSICVPGSSMPVDQMKKIFDQEYISGDGSS
                     DQLPSTSLGLYVSSEIVRKHGGAISLTSEKGKGSVFSVVLPFDKRRIAGLSAV"
     gene            complement(4802..6517)
                     /locus_tag="VGQ51_00400"
     CDS             complement(4802..6517)
                     /locus_tag="VGQ51_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV9035037.1"
                     /translation="MQHQCHRWWKEAVVYQVYPRSFQDSNGDGIGDLPGILTRLDYIV
                     SLGVDAVWINPVCLSAGKDVGYDIIDFRQVQPEYGTMEDMDRLIAAVHCKGLRLIMDM
                     VLNHTSNRHEWFQQASSSRDNPYYGYYIWWPVENGQPPARCGFFDPSGSAWTYNAPTR
                     SWYLHYFAPEQPDLNWDLPEVRSRLYDILRFWLEKGIDGLRFDALTYISKDQSFPAIT
                     PGILKEKYNGDWGHYYAAGPHLHEYLREMHREVLAPYNVLSIAEAAGVGADEALSFVA
                     EDRKEIDLLYPFEAIGIGYLPDSFKTPDPAGYSRGAWKEVYSRWSKVFDTEGWGALYL
                     GNHDQPRMVSRWGEDKDPFRQPSSKLLFTFLLTMRGTPFLYNGDEFGMTNIRFTSIED
                     YQDVETHGRFEQLQKDGGDTAAFLTDQQLAGRDNGRTPMQWSDAPGAGFTTGDPWLKI
                     NPDHTRINRESEEKDPSSVLHFVRMLIRLRRSHPALVYGDYTLMSGNDSPVHAYMRSH
                     RGCVLAILLNLSSEKQFFASILSAPSQGDILVDNYPGFQWSREGILLQPWQALVFACG
                     TPSKA"
     gene            6532..>7550
                     /locus_tag="VGQ51_00405"
     CDS             6532..>7550
                     /locus_tag="VGQ51_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV9035038.1"
                     /translation="MPDNQKQYLWWQDGIIYQVYPRSYQDSNGDGIGDLRGIIDRLDY
                     LKWLGIKAIWLSPVYPSPMADFGYDISDYTGIWPVFGSMADFDELLRLAHDAGMKLIL
                     DLVPNHSSDEHPWFFESRSSRDNPKRDWYIWKDAKPDGSPPNNWLSVFGGSAWEWDEK
                     TKQYYYHAFLVKQPDLNWRNPEVEKAMHQVMRFWLDKGVDGFRVDAMWHMVKDLQWRD
                     NPRNPDYKEWMGTYEQLLPVYSTDQPEMHGIVARMRKVLDEYDQRLMIAEIYLPVHRL
                     VAYYGSHDKEAHLPFNFMLISLPWDSRLIAASIDEYEAALPDGDWPNWVMGNHDQPRI
                     TSRVG"
BASE COUNT         1690 a         2054 c         2160 g         1646 t
ORIGIN      
        1 ttcttttatt tatatgcggt atcatggccc ggtgggagat tatacgggca gctaccctga
       61 tgacaggctg agccacgatt cggacaggat catcgcctgg ctcaaccagg ggaaagaggt
      121 atatgtctat ttcaataata ccatcggcga cgcactgaac aatgcggatc ggctgataga
      181 gctggtcggc gataccgtca accatatata gacccctttc ggagacgaag cgacataccc
      241 agccgtcgcg agaaatgact tcttatctcg gccaatatgg aaagagcgat ctcctcaggt
      301 gtggcggcgc cgatgtccag tccggcgggc gcgtatatgc gccgaaggtc ttcttctccc
      361 agcgggcagc cggcttcggt cagggcctcg aataaccggt ccgcgcgttt ccgcggtccg
      421 agcatcccga tataggttac gcaggtaggc agcacccgct gcaggttgag catgtcagtc
      481 ctatagtcgt gcgacatcag caggacggcg gtatattcat cgatgagggc agcgtcgggt
      541 tccttgctgt cgaggacggt cgtagccgcc tccacgaggg tcttttcgat ccgggccgtc
      601 tgaaggacga cggtcgagtc ccagcccagc tcacggattt gacgaagtaa tgtcctcata
      661 tcatagctgc cgccataggc aaccacatgg atggcgggaa ggatgatctc gatgaagaga
      721 tggaggtcgt cataaacgac gctgcgcgaa agacgttccg cctgcgcatg ccggatctcg
      781 gccagtacct tcggcccagg ctcgcccatg ggcagtatct cctcgaaaga aacgtcatcg
      841 gtatagagat atgttttccc cagcaattcc gggtcggggg ttcccgtgac cgtcacgacg
      901 accgaaggct gacggatacc ggtgaggccg gcaaggatac gaagcgggca gtccggatca
      961 tccgcttgca acggggtgaa aagcacgtcg atgattccct ggcaacccag accgacgccg
     1021 atctgataat catcgtcgcg ggtcgtatca taggtgatca cggatggacg attcagggcg
     1081 atgccctttt gggcacgccg gagggcatct ccttccagac agccaccgct gataccgccc
     1141 aggtaggtgc cgttgtcgag gaccagcata cgggcgcccg ccctgcgata ggaagagcct
     1201 tctatccgta cgacggtggc aagcgccgcc cgggtcgttt gaaagtcgat ggtttgccat
     1261 gcggcgacga tggtcctgat ctccttcatg agactaagtt acttcatttc cagtctccta
     1321 aacgacggcg aaggatgagg atcccgatgc gcatggggaa cagtcgccac agcccgggct
     1381 ataccgccga aagcccggcg atccgtcgtt tgtcgaaggg caggacgacc gaaaagaccg
     1441 agcccttgcc tttttcgctg gtgaggctga tcgcaccgcc atgtttacga acgatctcgg
     1501 aagaaacata gagccccagg ctagtgctgg gaagctgatc ggaggaaccg tcgccggaga
     1561 tatactcctg gtcgaatatc tttttcatct ggtcgaccgg catgctggaa cccggtacgc
     1621 agatcgaaag gcggacatgc tcttgtgtca cgacggtacg gatcgcgatc tgtccggcat
     1681 gatcggaata ttttgcggcg ttcgaaagaa gatttccaag gacacgccct atcctctccc
     1741 tgtctccctg caaaggcggc gatgacggca acgggtcgac gaaaatgggg acggagctcg
     1801 tcgtttgtgt cgcttcggcg atctcggtga tcaaagtgtt gagatcgaaa ggtacttcgt
     1861 ctataacgat cctgcctctt gtgatccggc tgacatccag cagatccctg gtcaacccgg
     1921 ccaggcgatc gacctgactg ttcagctttc cgacaaggaa ggcggatctc ttatcgccgt
     1981 tgtctgtcag ctcctgcgaa agcatctcgg aataggcccg gatactggta gccggtgtcc
     2041 tcagctcgtg gctggcgagg ccaatgaggt tgtctttgaa gcgttggagg tcccgttgtt
     2101 cggtgacgtc gtcaatggac aaaaggatgc cggttcgaag accgggcagt ccggccatcc
     2161 gggtggcgct gaaatggagg agcttttctt tgccgtcacg ggtatcgtac ctgagctcga
     2221 attccgctcc cagttggtct ttcagtacgt gctgcaagcg gaaccggata ctgtcctttc
     2281 taaacaggtc cggacccagc cgggtcagat actggccgac cgcctcgtgc gatgccaggt
     2341 cgaacagatg cagaaaggcc cggtttacgg tatggatccg caggtcatct cgcaaaacga
     2401 cgatcgaccg gctgaagctc gcaacgatcg ccatcgcagc atcggcgacc gaatggatct
     2461 cccgaatttg cgtgcgaaga ccttcgttca agacattcaa ttctttatac tttgtctgca
     2521 tttctccgta catttcattc agctcgccgt tcaattcctg caactcttta ttgaggctcc
     2581 ggaactcttc gttcgaagcc tgcaggtctt tattggcccg ctggagtccg gactgctgcc
     2641 ttgtggcatc ccggtccgcg accagcaaaa gaccccgcat ctcctgtatt tccttttcta
     2701 tcgattgtct ccggaggcct ttgagagacg acaccccgtc cgacccgtct tcgcgggaag
     2761 gttcagcctc ccgtcgccga gctatctcgc ggatgatcac caatttccgg ttaacgccga
     2821 acgaacggag cggcaccatt tcaacggata cttctttgct attcgaatct tcgcccaact
     2881 ggatgccctt ccgggtaatg gcccgtcctt ctttgtcggt caagtcgatc agatcattga
     2941 gctcgaatac cagctcgtcc tttacgattc ggaacaggga gcgcgacgat ctctcccggc
     3001 aggcgcggag atagggttcg gtattgccgt aataccggat aaccctgaat tgctcatcta
     3061 cgaggaagga tgccggcaca tagccggaaa gcaacagctt atcagcctcc agttctccgg
     3121 agtccgccat gtttgcgggt ccctggtcgt cctgccacag gttcacccgg acgtccgctt
     3181 ttaggtatac tccgtgatat ccttgcgaag cgatatacag ttctgcggga atgccatgcg
     3241 ggtgaagcac atttgcgatg aagtaaccgt ccggggtaag cgcatagtga aaaagccgga
     3301 atgctctggc gcaatccccg gagccaaagc cgatgagcga gccggattcg atgacgacgt
     3361 ccatataaga gaacggagag tccttcagca ggttgtgcga ggcgaagata catttctccc
     3421 gaagggattt gaccacctgc caatccttgt ctttttttat aaagaatttc ttccgccggt
     3481 tgcgcgtgac atagtgcatt tcctcattgg tgaaaatacc ttttctcgcc ttcacgacgg
     3541 cattgttgtc aaaagaggtg acgaataacc ggacggccag gttcgattgg caggcttcca
     3601 ggtattccat gatgaccata gcgatggaat aggcctgatg gccgcctgta caatgcggca
     3661 gccagacgcg caacggcgcg gttcgttgtg cggaatcgag caatctcggt aacacggaca
     3721 cgctcagcgc tgtttccagg ccggcatcga tgacgaaacc gctgagggca gattgcaatt
     3781 cgtcctcgag gtgcatcaac tcctcttcct cggtctgaag cacggtagta tacgcgttgg
     3841 ggcataccat cccgcggatc gccattcttc gatgaatgcg ccggacgaca tcttcccggc
     3901 gatatcgtgc ataatcacag cctgatcgat gcagcaacag cgggtagacg gagctcaagt
     3961 caacctccgt gcgtggtcca caatggtcct ccggcggttg ggaatattcg accagtccac
     4021 ccagccagga tgcggcgagc ggggccgtca ggaacagatc gaccgccctg tacgccgggt
     4081 gaggcgacag aaatggatcc tgttcatcca gcactattgt aaaacctcct tcgtccctga
     4141 ccacccgaag acccggcagg acacgggcgg ccatatcggg aagcaggacg gctgcacagc
     4201 gtccggggca tccggcgccg acggatgcca ggaacagatc ggggaggtaa tcaatcccgc
     4261 catccgcgac gggcccctgg aaaataagcc ttcccctgac cagttggatc gcgcttcccg
     4321 gaggaagcag atatatacaa tctttttcaa gcaagccatc ctgcacgggc atacgcaatg
     4381 gcaggagcgt cgtcctttcc agcagtgctg cgaggctttc cgccccgtcg tccgcacggc
     4441 gtcctcccca agatccatga tcttccatga tcaggtagct gatccctaat gaaacaggca
     4501 actggcgaaa gaacgtttcg aaaaccgaag catcacctgc cggaatgatc attgcgacga
     4561 caggaggcga cggatgaggt tcttcgggat ccgaagcgat ctttaaatga ggagagggcc
     4621 tttgtggcat agtatccttt ttgtatttaa atcgtcattc cacagtcgaa tcgcagggga
     4681 cagagggcta ttactaacgg taagggataa ccggcatatg cggatggccg ggtgaaatag
     4741 tgagaatacg actaccggga ttttagcaag catggcaatt tagtacaaat atgacataaa
     4801 atcaagcttt tgacggggtt ccgcaggcga agacgagagc ctgccagggc tggagaagga
     4861 taccctcgcg cgaccattga aacccggggt aattatcgac caggatatcg ccctgggaag
     4921 gcgcggacag gatactggcg aaaaactgtt tttccgaaga gaggttcaac agtatggcaa
     4981 ggacgcatcc ccgatgcgag cgcatataag catgaaccgg actgtcgttc ccggacatca
     5041 gggtatagtc tccatacacc agcgctgggt gcgatcggcg caggcgtatc agcatgcgaa
     5101 cgaaatgaag taccgaggaa gggtcctttt cttccgactc acggtttatg cgtgtgtgat
     5161 cgggattgat cttcaaccac ggatcaccgg tggtgaatcc ggcgcccggc gcgtcgctcc
     5221 attgcatcgg ggtgcggccg ttatcccggc cggccagctg ctgatcggtt agaaaggcgg
     5281 cggtatctcc cccgtctttt tggagctgtt caaaacgtcc gtgcgtttcg acgtcctggt
     5341 aatcttcgat cgaggtgaac cggatattgg tcatgccgaa ttcatcaccg ttgtataaaa
     5401 agggtgtgcc gcgcatggtc agcaaaaagg tgaacaacag tttggaagat ggttggcgga
     5461 aggggtcctt atcctcgccc caacgcgaga ccattcgagg ttggtcgtga ttgccgagat
     5521 acagcgcgcc ccagccttcc gtgtcgaaga ctttcgacca cctgctatat acctccttcc
     5581 aggcccctcg gctataccct gccgggtcgg gggtcttgaa cgagtccggc aggtatccga
     5641 tgccgatggc ctcgaacgga tacagcagat cgatctcctt ccggtcttca gctacgaagg
     5701 atagtgcctc atccgcaccg acaccagccg cttccgcaat agacagaaca ttgtagggtg
     5761 cgagcacttc gcggtgcatt tcccgcaaat attcatggag atgcggcccc gccgcgtaat
     5821 aatgtcccca gtcgccgtta tatttttctt tcaggatacc gggggttatc gcgggaaaag
     5881 actggtcttt ggagatatag gtcagcgcat cgaagcgaag accgtcgata cctttttcga
     5941 gccagaaccg gaggatatca taaagcctgg aacgtacttc cggaagatcc cagttgagat
     6001 cgggctgctc cggggcgaag taatgcaggt accaggatcg ggtaggcgca ttgtaggtcc
     6061 aggcgctgcc ggacggatcg aagaaaccgc aacgggcggg cggttggccg ttttcgacag
     6121 gccaccagat atagtaaccg tagtagggat tgtcacgcga gctgctggcc tgttggaacc
     6181 actcatgccg gttgctggta tgattgagca ccatatccat gatgagccga agacctttac
     6241 agtgtacagc ggcgataagg cggtccatgt cttccatggt cccatattcc ggctgcacct
     6301 ggcggaaatc gatgatatcg taccccacat ccttgccggc ggacagacaa acaggattga
     6361 tccagacggc atcgacgccc agcgagacaa tatagtcgag acgcgtaagg atacccggaa
     6421 gatcaccgat gccgtcgccg ttgctatcct ggaaactgcg cggatatacc tggtacacga
     6481 cggcctcttt ccaccagcgg tggcattggt gttgcattgg gcacatttat tatgccggac
     6541 aatcaaaaac aatacctctg gtggcaggat gggatcattt atcaggtgta cccacggtcc
     6601 taccaggaca gcaatggaga cgggataggc gatctgcgcg gaattatcga ccgattggat
     6661 tatctgaaat ggctggggat caaagcaatc tggctatcac cggtctatcc ctccccgatg
     6721 gccgatttcg gttacgatat ctccgattat accggtatct ggccggtgtt cggctcgatg
     6781 gcggattttg acgagctgct gcggctggca catgatgcgg gaatgaaatt gatcctcgat
     6841 ctggtgccga accatagttc ggacgaacat ccctggttct tcgaatccag gtcttcgagg
     6901 gacaatccca aacgcgactg gtatatttgg aaggatgcca agcccgacgg cagtccgccg
     6961 aacaattggc tcagcgtttt cggcggcagc gcctgggaat gggatgagaa gacgaagcaa
     7021 tattattatc acgcatttct cgtcaaacag cccgatctca actggcgtaa tcccgaagtg
     7081 gaaaaggcga tgcaccaggt gatgcgtttc tggctcgata aaggcgtgga cggtttcagg
     7141 gtggatgcca tgtggcatat ggtcaaggac ctccaatggc gcgataatcc ccggaacccc
     7201 gattataagg aatggatggg tacgtatgag cagttattgc ctgtctattc gaccgatcag
     7261 ccggagatgc atggcatcgt cgcccggatg cgcaaggtgc tggatgaata tgatcagcgg
     7321 ctgatgatcg ccgaaatata cctgcccgtg catcgtctcg tggcctatta cggctcgcat
     7381 gacaaggagg cgcacctgcc gttcaatttc atgctcatca gtctgccatg ggactcgcgt
     7441 ctgatagccg caagcatcga tgaatatgag gccgctctgc ccgatgggga ctggcccaac
     7501 tgggtaatgg gtaaccacga tcagccgagg atcaccagtc gcgtcggtat
//
LOCUS       DASYSV010000013         6859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1028300, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000013 DASYSV010000000
VERSION     DASYSV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028300"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VGQ51_00410"
     CDS             complement(<1..793)
                     /locus_tag="VGQ51_00410"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV9035039.1"
                     /translation="MERYTAMTGHFFDPMLAGRFNGSILVAKNGVILFEKYKGFRNPR
                     RKQDSITEHTSFHLASISKTFTAMGTLKLWQEGKLDIHDPVSKYLPGFPYPQITIQTL
                     LNHRSGLHNYVHFMDKPGVDKRRFLNNGDVLKYIIDHPGSREIFSGVANRHFEYSNTN
                     YALLALIIEKVSGLPYPEFLAKTFFEPLGMRDTYVNTLADSAKSMASYYQTGRPFRME
                     FLDLVYGDKNVYSTVRDMYKWDQALRSGQLFSKGVLDSAYSGYSFE"
     gene            1037..2275
                     /gene="serA"
                     /locus_tag="VGQ51_00415"
     CDS             1037..2275
                     /gene="serA"
                     /locus_tag="VGQ51_00415"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEV9035040.1"
                     /translation="MAEKKTTSYPKEKINILFLENISDAAVKHFKDSGYASVRKLGGA
                     LSEDQLIQEIKDVHLLGIRSKTQITQKVLDAAKKLQAIGCFCIGVNQVDLKAATNAGV
                     VVFNAPYSNTRSVAELVIGLSIMLIRRIPDKSRAAHDGIWAKEAKGSFELRGKTLGII
                     GYGNIGTQVSILAESMGMKVIFYDTVTKLPLGNAEAKKTIRDVVSQADIVTLHVPDTP
                     QTKGMINKTVLKQFKKGAILLNYARGEVVDLETLAKCLQEGMLSGAAIDVYPWEPEKN
                     GDRFTTPLQGLSNVILTPHIGGSTEEAQENIGEDVSNKLFQYLETGMTIGSHTVPPLS
                     LTHQEGTHRILHIHNNVPGVLSEINSTLSKHNINILGQYLKTNDQIGYVVLDIDKRLS
                     NQALQLLKSVRATIKTRLLY"
     gene            2458..3447
                     /gene="mgrA"
                     /locus_tag="VGQ51_00420"
     CDS             2458..3447
                     /gene="mgrA"
                     /locus_tag="VGQ51_00420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEV9035041.1"
                     /translation="MSYTPLDNRYDAMEYRRCGRSGLLLPAVSLGLWHNFGYVDTLEN
                     CREILHLAFDSGITHFDLANNYGPPPGSAEENFGRILKRDFHHYRDQLIISSKAGYYM
                     WPGPYGEWGSKKYLVSSLDQSLGRMGLDYVDIFYHHRPDPNTPLEETMMALDLIVRQG
                     KALYVGISNYQAPEAARAIQILRQLGTPCLIHQPKYSMFERWVEGGLLDLLEKEGVGC
                     IPFSPLAQGLLTNKYLKGIPEDSRAHKSTGFLQEKEVTPEKVQKILRLNELAIKRDQT
                     LAQMALAWILRDKRITSVLIGVSKPEQVTDSLQCFRNTWFSGEELAEIENILK"
     gene            3632..4105
                     /locus_tag="VGQ51_00425"
     CDS             3632..4105
                     /locus_tag="VGQ51_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035042.1"
                     /translation="MKQFITYTFSLLFCAAVQANPGPHAATGSANIQSKALVNAGAVH
                     FQFKNITAAAEHKDSVLIIFDRFDRTGAGIVYQVFAADTTDGIDISQVPAGKYYVTIQ
                     CLGLHHDRLERVVTIKSQKSEKVRVQLTPSEEFSKDKVVIPAYRPDFSDMDIKSK"
     gene            4520..>6859
                     /locus_tag="VGQ51_00430"
     CDS             4520..>6859
                     /locus_tag="VGQ51_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEV9035043.1"
                     /translation="MENKPVAKDTAPAEESLLLKTPQIELPKGGGAIKSIDEKFSVNA
                     VNGTGSVSIPLPFSSARGFSPSLSIAYSSGNGNDIFGMGWSLTLPSIKRKTEKELPRY
                     FDATDSDTYLIAGAEDLVPIFKTNNNGAFIQDKNGNYIVDDGESPDTLFQIRPYRPRV
                     EGAFSRIERWTEKATGHIHWRVISKDNGTTLYGLTAATRISDPADDRKIFQWLPEFTF
                     DDKGNCAKYEYKNEDQTGIGKSCLHNRNRLNGKAPFMNTYLKRVWYGNILPYKNPGDP
                     FPDRFMFENVFDYGEHDTTILPFNETGVWSFRPDAFSSYKAGFEIRTCRLCHRVLLYH
                     HFDELPGGSALIKSLQFAYDDNGEDGFTFLKEVTFTGYTKHDDGSYSQKSFPPLSYSY
                     SALAWDTTVKNLTPEDLANAPAGIDNVNYQFVDLYSEGLSGILTEETGGWYYKGNLRE
                     GHFTPATLVMTKPSFTGLRQNWQLIELEADGIKQMVNRHLAPKGFFEISDGEQWQPFQ
                     SFESLPNIDFTDPYTRLLDLDGDGMADVLITDQDVFTWYPSKGKKGFGDRYRVPNTPD
                     EELNPAIVFADEIQSIFLADMTGDGLTDIVRIRNGEVCYWPNLGYGRFGARVSMDNAP
                     VFDHDELFTPLLLKLADIDGSGTADLVYLGQNQVRIWLNQQGNTFLPEPRTIPFFPET
                     GNVTKIDIVDLLGTGLSCIVWSSPLPEDQTSPLRYIDLMDSTKPHILTGYKNNLGKEV
                     QLGYTPSTKFYLEDKLGGTPWITKLHFPVHCVSKVIVYDR"
BASE COUNT         1806 a         1798 c         1638 g         1617 t
ORIGIN      
        1 tttcaaaact gtatccggaa taggcgctat ccagcacacc ctttgaaaac agctgcccag
       61 aacgcaaagc ctgatcccat ttgtacatat ccctgactgt gctatataca tttttatcac
      121 catataccag gtcgaggaat tccatccgga atggccgccc ggtctgataa tacgaggcca
      181 tcgatttcgc agaatcggca agcgtattga cataagtatc ccgcatcccc agcggctcga
      241 agaaggtctt ggcgaggaat tcggggtagg gaagcccgga gaccttttcg atgatcagcg
      301 ccaaaagcgc ataattcgta ttggagtatt cgaaatgccg gttggcgacg ccactgaaga
      361 tctcgcgcga tcccggatga tcgatgatat attttaagac atctccatta ttcaggaacc
      421 ggcgtttgtc tacgcccggt ttatccataa aatggacata attatgcaga ccgctgcgat
      481 ggttcaaaag ggtttggatc gtgatctggg ggtaggggaa ccccgggagg tatttggaca
      541 ccgggtcatg aatatccagc ttgccttcct gccaaagctt gagggtgccc atggcggtaa
      601 acgtcttgga gatggaagcc aaatgaaatg aggtgtgctc ggtgatggaa tcctgtttgc
      661 gccggggatt gcggaagccc ttgtattttt cgaaaagtat tacaccattt ttggccacca
      721 ggatactgcc gttgaaacga ccggccagca tcggatcgaa aaaatgcccg gtcatcgccg
      781 tatagcgttc catttccgta ctcgttaaag ggggaacggc aggcggagca ggcgccatgg
      841 tgtctgccct gacaataggt tttgcagtct gagcctgacc acacgacacg accactaagg
      901 ttacggataa agccaaatag ccaatacctt tcacgacagc gaaacttttt tgggttagaa
      961 aacggcaaaa ttaaaagaat ccggtgttac tttgcacttc caattcggaa agtaacgcaa
     1021 aaagagaggc aaaattatgg cggagaaaaa aactacaagt tatcccaaag aaaagattaa
     1081 cattctcttc ttagaaaaca tcagcgatgc ggccgtcaaa catttcaaag actctggata
     1141 tgcctccgtt cgtaagctgg gcggagctct ttcggaagac cagctcatcc aggagattaa
     1201 agatgtgcat ctgctgggga tccggtctaa gacccaaata acccagaagg tgctggatgc
     1261 cgctaagaag ctacaggcga ttggctgctt ttgcataggc gtcaaccagg tagacctgaa
     1321 agccgccacc aatgccggag tggtggtctt caacgctcct tattccaata cccgaagcgt
     1381 ggccgaactg gtgatcggtt tatccatcat gctcatccgg cgtattcccg acaaaagcag
     1441 ggccgcccat gacggcattt gggccaagga ggccaaaggc agcttcgagc ttcgaggtaa
     1501 aacattggga atcataggtt atggaaatat cggcacccag gtgagcattc tggcggaatc
     1561 catgggcatg aaagtcatct tttatgacac ggtgacaaaa ctgccgctgg gcaacgcgga
     1621 ggccaaaaaa acgatcaggg acgtagtaag ccaggcggat atcgtcaccc tgcatgtacc
     1681 cgataccccc caaacaaagg ggatgatcaa taaaacggtc ttaaaacagt ttaaaaaagg
     1741 agcaattttg ctcaattatg cacgtggtga agtggtcgat ctcgaaacgc tggccaaatg
     1801 tctccaggaa gggatgctgt ccggtgccgc catcgacgtg tacccctggg agccggagaa
     1861 gaacggcgac cggttcacga ctccgctaca aggcctttcc aatgtcatcc tgacccccca
     1921 cataggcggg tccactgaag aagcccagga aaacatcggc gaggacgtca gcaataaatt
     1981 attccaatac ctggagacag gcatgaccat cggaagtcat accgtgccgc ctctgagcct
     2041 gacacaccag gaaggcacac accgcatcct gcatatccat aacaatgtgc cgggcgttct
     2101 ttcggagatc aatagtaccc tttccaaaca caatatcaat atcctggggc aatacctcaa
     2161 gaccaatgac cagatcggct atgtcgtgct ggatatcgac aagcggttgt ccaatcaggc
     2221 tcttcaactg ctcaagagcg tgcgggctac gatcaagaca agattgttgt actaaaagcc
     2281 gataaaaatg gcggatcttt ttcgcgcggc tacggatcgc cggtgtggcg cgttcccaac
     2341 cgatataccg gacgatgcgg tcggtttgct ttttgctgtg ttcctgtcgg agcaactgct
     2401 caagaccgat gggcatgtgg taaagttgat taaatttaac gcataatttt cttccatatg
     2461 agctatacac cactcgacaa tcgttacgat gccatggaat atcgccgttg cggtcgcagc
     2521 ggtctgcttc ttccggcggt gtcgcttgga ctctggcata acttcgggta tgtggatact
     2581 ctcgagaact gccgggagat ccttcacctg gcttttgatt ccggcataac gcatttcgac
     2641 ctcgctaaca actatggacc gccgccgggc tcagcggaag agaatttcgg ccgcatcctg
     2701 aaacgcgatt ttcatcacta tcgcgaccag ttgatcattt cctcgaaggc cggttactac
     2761 atgtggcccg ggccctatgg cgaatgggga tccaaaaaat acctcgtttc aagtctcgat
     2821 caaagcctcg ggcggatggg actcgactat gtcgatattt tctaccacca ccgtcccgat
     2881 cccaatactc cgctggagga gaccatgatg gcattggacc tgatcgttcg ccagggaaag
     2941 gcgttgtatg tcggtatatc gaattatcag gcccccgaag cagcgcgggc gatacagatc
     3001 ctccgtcagc tgggtacccc ctgtttgatc catcagccca aatattcgat gttcgaacgg
     3061 tgggtggaag gcggactact cgatctcctc gaaaaggaag gggtgggatg tatacccttt
     3121 tctccgctgg cgcaggggct gctgaccaac aaatacctga agggcatccc cgaggactcg
     3181 agggcgcata agtccacggg gtttttgcag gaaaaagagg ttaccccgga aaaagtgcag
     3241 aaaatattac gactcaatga attagccatc aagagggacc agacgctggc gcagatggct
     3301 cttgcctgga tattgagaga caagcggata acttccgtgc ttatcggtgt cagcaagccc
     3361 gaacaggtga ccgattcatt acaatgtttc cgaaatacct ggttctccgg cgaagagctc
     3421 gcggagatcg aaaatatcct gaaataaata atccgaagat ccgcagtatc gcaatggttg
     3481 tcaatcaata gacaaccatt tctgctttcg ggactaccca ttcccatcag gggaaacatt
     3541 aaacttatgg caatttcgta agtctatcat tataagcagt gtatgatttg gcactgggat
     3601 tggatttatt agatcataat cgttaccctt tatgaaacaa ttcatcacct acacctttag
     3661 cctgctcttc tgcgctgcag tgcaggcgaa ccccgggccc cacgcggcta ccggaagcgc
     3721 caatatccaa tctaaagccc tggtcaatgc tggagccgtt catttccagt tcaaaaatat
     3781 caccgctgcc gctgagcata aggattcggt cctgatcatt ttcgatcgtt ttgaccgtac
     3841 gggtgccggc atcgtttacc aggtgtttgc agccgatact accgatggca ttgatatttc
     3901 gcaggttccc gccggcaaat actacgtcac cattcagtgt ctgggtctgc accacgaccg
     3961 cctcgagaga gtcgttacca tcaaatccca aaaaagtgaa aaggtccgcg tccaactaac
     4021 gccttccgaa gaattttcca aagacaaggt cgtgatcccg gcttatcgcc ccgatttttc
     4081 cgatatggac atcaagtcca aataatccgc cgatatccct cgctgttgtt attaatcctt
     4141 tatctgcttt ttcgatcgtc ccgatcaata tccttcccaa agctgcaata tcctccacag
     4201 caataaccgt atctccacta atataaggct tggacaagcc attgttccag gccttctttt
     4261 ttgtcttata attaacatta tgttaaatag tacttcccga tgccatacgg ccaccaacca
     4321 gccaatgaca ggcaatttgc gtacttctac ttgtcgaaat ccaaattgtt aatcctacct
     4381 tagaaatacg gtacccgcgc tcgacatttc tagctactct accacaccat cctgatcacc
     4441 gcctgtaacg cttctgaaac agtagccgtc tccctgacga atggactaat atcttagctg
     4501 gcgatctatt tatttacata tggaaaacaa gcccgttgct aaagacaccg ctcctgccga
     4561 ggaatccttg cttttgaaaa caccccagat cgaattaccc aagggcggcg gcgccataaa
     4621 aagcatcgac gaaaaatttt cggtgaacgc ggtgaatggc accggttccg tcagcatacc
     4681 ccttcctttc tcttccgcac gaggattttc tccgtcgctc tccatagcct atagttcagg
     4741 caacggcaac gacattttcg gcatgggctg gtcattgacc ctcccttcca tcaaaagaaa
     4801 gaccgaaaaa gaactgcccc gctatttcga tgccacagat tccgacacat accttatcgc
     4861 cggtgcggag gacctggtgc ccatatttaa gacaaacaat aacggagctt ttatacaaga
     4921 caagaacggg aattatatcg tcgatgatgg agagtcgccg gatactttat tccagatccg
     4981 gccctaccgt ccccgtgtgg aaggggcgtt ttcccgaata gaaagatgga ccgaaaaagc
     5041 taccggacac atccattggc gcgtcatttc aaaagataat ggcaccactc tttatgggct
     5101 cacggctgca acaaggatct ctgatccggc ggatgacagg aagatctttc aatggttgcc
     5161 agaatttaca ttcgatgata aagggaattg tgccaaatat gaatataaga acgaagacca
     5221 aaccggtatc ggcaagtcct gcctgcacaa caggaaccgg ttgaacggca aggcgccatt
     5281 catgaatacc taccttaaaa gggtctggta tggtaatatc ctgccttaca aaaacccagg
     5341 cgaccctttt cctgaccggt ttatgttcga aaatgtgttc gactatggag aacacgatac
     5401 aaccatcctc ccttttaatg agaccggcgt ctggtctttc cggcccgatg ccttttcttc
     5461 ctataaggca ggattcgaga tccgaacctg ccgtctttgc caccgcgttc tcctttatca
     5521 tcatttcgat gaattaccgg gcggttcggc cctgatcaaa tcattgcagt tcgcttacga
     5581 cgacaatggg gaagacggtt ttactttttt gaaagaagtt acctttaccg gctataccaa
     5641 acatgacgat ggtagctata gccaaaaatc ttttccgcca ctttcctata gctacagtgc
     5701 gcttgcctgg gacaccacgg taaaaaacct tacgccggaa gatctggcca atgcccctgc
     5761 cggcatcgac aatgtcaatt accagtttgt cgatctgtat agtgaagggc tttccggtat
     5821 cctcaccgaa gagacgggcg gctggtatta taaaggcaat ctccgggaag gccacttcac
     5881 tcccgcaacg ctggtcatga cgaaaccgtc cttcacgggt ctaagacaga attggcagct
     5941 gatcgaattg gaggccgacg gtataaaaca gatggtcaac cgacatctgg cgcccaaagg
     6001 atttttcgaa ataagcgacg gcgaacagtg gcagcccttc caatctttcg aatccctgcc
     6061 caatatcgat tttaccgatc cctatacccg cctgctcgat ctcgacggcg acggaatggc
     6121 cgacgtcctg atcaccgatc aggatgtatt cacctggtat ccttccaaag ggaaaaaagg
     6181 ctttggcgac aggtaccgtg tgccaaatac cccggacgaa gagctgaatc ccgctattgt
     6241 cttcgcggac gagatccaaa gcatcttcct ggcggatatg accggagacg gtctcaccga
     6301 tattgtgcgt atccgcaatg gcgaagtctg ctactggcct aatctcggct atggaagatt
     6361 cggcgccagg gtaagcatgg acaatgcccc tgtttttgac cacgatgaac tcttcacccc
     6421 tttactgctg aaactggccg atatagacgg ctcgggaaca gcggatctcg tttacctggg
     6481 tcagaaccag gtacgcatat ggctcaatca acagggaaat acttttttac ccgagcccag
     6541 aaccatccct tttttccccg agaccggaaa cgttacgaaa atagacattg tcgacctgtt
     6601 gggcaccggc ctgagctgca tcgtctggag cagccccctt ccggaagatc agacctcgcc
     6661 cctgcgttac atcgatctca tggatagcac aaaaccgcat atcctcacgg gttataagaa
     6721 caacctcggc aaggaagtgc agctcgggta tacgccctcc acgaagtttt acctggaaga
     6781 caagctcggt ggaacgccct ggataaccaa actgcatttc cccgttcatt gtgtatccaa
     6841 ggtgatcgtg tacgacagg
//
LOCUS       DASYSV010000014         3216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2052735, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000014 DASYSV010000000
VERSION     DASYSV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052735"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGQ51_00435"
     CDS             <1..289
                     /locus_tag="VGQ51_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035044.1"
                     /translation="AFQAAGYDSIGAIDSANVDTLIQLALNQVVKGSYFTTTFPSTVT
                     NMTGAGILVTRSNGILQFAGGGNVTPVHWLSGDNVAGQSTILHKTDGIVSP"
     gene            378..1748
                     /gene="mgtE"
                     /locus_tag="VGQ51_00440"
     CDS             378..1748
                     /gene="mgtE"
                     /locus_tag="VGQ51_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEV9035045.1"
                     /translation="MRNFNDIKNLLQRNKIADFVKQNKLTPRDIANFANRHTQSAVKL
                     FQQVDPTHAARAFKFMRRKKQEDIIKSLPEEKAAELLNAMQPDDRTAFLEELPGKYVT
                     ELLKILTPENRTETLELLGYQEGSVGRLMTPDYVAINENMTCQEVLSTIRHRGQASET
                     LNWLFVVDDKGLLIDDINIKDFLIVEPGTRVSQIMDRQFLALNVSDTQKNAINIFRNH
                     SRYALPVTDTNGVLLGIVTIDDVLRLAESEGTREIQKIGGSEALDEPYTTISFARLIK
                     KRAGWLIILFLGEMLTATAMGHFQDEISKAVVLALFIPLIISSGGNSGSQASSIIIRA
                     MALGEVGFRDAWRVVRREFASGLVLGLILGIVGFLRISIWQEFNIGGGYGEHWLRVAT
                     TVFFALIGVVLWGTLSGSMLPLFLKKLGADPAASSAPFVATLVDVTGLIIYFTVAFIV
                     MQGTLL"
     gene            complement(1791..2309)
                     /locus_tag="VGQ51_00445"
     CDS             complement(1791..2309)
                     /locus_tag="VGQ51_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HEV9035046.1"
                     /translation="MSDEIINKVAQSGLSTLDLEDYYPKEEIVVFDLKPMLFREMILK
                     EKEFRAALQTVDWAGYQGKILAITCTADAIIPSWAYMLVAVQAGPYAKSIILGDRDTA
                     LRETFLDNLRAIDIQAFADQRVVVKGCGDRVDGSSVGGFAYLEIARRLRPVVKSMMYG
                     EACSNVPVYKKK"
     gene            complement(2375..>3216)
                     /locus_tag="VGQ51_00450"
     CDS             complement(2375..>3216)
                     /locus_tag="VGQ51_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV9035047.1"
                     /translation="GKKGETKIVKSQYGYHYIEILDQKNFETAYKIAQLSRKIETSTE
                     TDQAASGLASQFAGQSRNAQAFEANAEKQHLQKQFAPDIAPTAFSIPGLGINRQLVRW
                     AYDADLGNVSEPFPVGDKYVVVLLTEINKEGTMSVAKARAQIEPILRNKKKAEQIMKK
                     DGSPASLDAAASTTGQTIQHADSVVFAAAFNRLFGQEPKVVGSAFNKQLAGKPVSPAI
                     AGNGGVFFIKVENVSAMSNPNADPQQQRFMQEQRIRTQIYQQLLEAERKMASIKDYRS
                     RFF"
BASE COUNT          684 a          891 c          860 g          781 t
ORIGIN      
        1 cgccttccag gcggcaggat atgacagcat cggggccatc gactccgcta atgtcgatac
       61 ccttatccag ctggcgttga accaggtggt aaagggcagc tatttcacca cgaccttccc
      121 ttctacggta acgaatatga cgggcgcagg catcctcgtt accaggagta atggcatcct
      181 gcagtttgcc ggcggaggga atgtgacgcc ggtccattgg ctcagcggcg ataatgtggc
      241 cggccagagc accatcctgc acaagacgga tgggatcgtg agcccctaac cccttgggcc
      301 cgcagtacgc ggcgcttttc atttccgtaa cattacattt tggttacatt cgttaaattt
      361 atgcactctc atcagtcatg cgcaatttca acgatatcaa aaacctgctt caacgcaaca
      421 agatcgcgga tttcgtcaaa cagaacaagt tgactccgcg ggacatcgcc aatttcgcca
      481 accggcacac gcaaagcgcg gtcaagctct tccagcaggt ggaccctacg catgctgcac
      541 gcgccttcaa gttcatgcgg cgaaaaaagc aggaggatat catcaagtca ctgcccgaag
      601 agaaggcggc cgagctgctg aacgccatgc agccagatga ccggactgcc ttcctcgaag
      661 agctgccggg taagtacgtg actgagctgc tgaagatcct gacgcctgag aaccgcaccg
      721 agacgctgga attattgggt tatcaggaag gttcggtggg ccggttgatg acccccgact
      781 atgtcgcgat caatgagaat atgacctgtc aggaagtcct ttcaacgatc cgccatcgtg
      841 gtcaggcatc ggagaccctg aactggcttt ttgtggtgga tgacaaaggg ttgctgatcg
      901 acgatatcaa tatcaaggac tttctcatcg tggagcccgg aacccgggtc tcgcagatca
      961 tggatcgcca gttccttgcg ctgaatgtca gcgatacaca gaaaaatgcg atcaacatct
     1021 tccgcaacca tagccgttat gcgctgcccg ttaccgatac caacggggtc ctgctgggta
     1081 tcgtaacgat cgacgatgtc ctgcgactgg cggaatccga gggaacacgt gagatccaga
     1141 agatcggtgg ttccgaagcc ctcgatgagc cttatacgac catctccttt gccagactga
     1201 tcaagaaacg cgcgggatgg ctgatcatcc ttttcctggg tgagatgctc acggccactg
     1261 ccatggggca tttccaggac gagatcagca aggcggtggt actggcgctg ttcattccgc
     1321 tgatcatttc cagcggtggc aattcaggca gccaggcttc gtccatcatt atccgcgcca
     1381 tggcgctcgg tgaggtcggt ttccgcgacg cctggagagt cgttcgcagg gaattcgctt
     1441 cggggctcgt gttggggctc atcctgggga tcgttggttt cctgcgtatt tccatctggc
     1501 aggaattcaa catcggggga ggttatggcg aacactggct aagggtcgcc accactgtct
     1561 tttttgcgct gatcggggtc gtattatggg gaacactttc cggcagcatg ctgccgttgt
     1621 tcctcaaaaa gctgggtgcg gaccccgccg cttcctccgc acctttcgtg gccacgctgg
     1681 tcgacgtcac cggtcttatc atttatttca cggtcgcgtt tatcgtgatg cagggcacat
     1741 tgctttaata gctcatctca tccagtttca cctttctttt gaaagttatt ctatttcttc
     1801 ttatagacag gcacattgct gcaagcctcc ccatacatca tgcttttcac gacgggcctc
     1861 aaccgccggg cgatctccag gtatgcaaac ccaccgactg aagacccatc gactcgatcg
     1921 ccgcatccct tcaccacgac ccgttggtcc gcaaacgctt gtatatcgat agcacgaagg
     1981 ttgtccaaaa aagtttcccg caacgccgtg tcacggtcac cgagaatgat gctctttgcg
     2041 tagggtccgg cctgtacggc cacaagcata taggcccagg aagggatgat ggcgtccgcg
     2101 gtacaggtga tggcgagtat tttaccctgg tatccagccc agtccacggt ctgaagcgcg
     2161 gcgcggaatt ccttttcttt caggatcatt tcccggaaca acatcggctt aagatcgaac
     2221 acgacgatct cctccttcgg atagtagtcc tcgagatcca gcgtcgatag gccgctttgc
     2281 gccactttat tgatgatctc atcgctcacg ccgcaaagat acgaaaaaaa gaacccccct
     2341 ctgagagggg gggttcccgg tttgttttgc tccgttagaa gaacctactg cgataatctt
     2401 ttatacttgc catcttacgc tcggcttcca atagttgctg atagatctgc gtgcgtatcc
     2461 gctgctcctg catgaaccgc tgctgctgcg gatcggcatt ggggttggac atggcgctga
     2521 cattctccac ctttataaaa aatacacctc cgttgccggc aatggcggga gataccggct
     2581 ttccggccag ctgtttgttg aaagcagaac ccacgacttt gggttcctgt ccgaacaggc
     2641 gattgaaggc ggcagcaaaa acgacgctgt cggcatgctg gatcgtctga cccgtggtgc
     2701 tggcggcggc atccagcgaa gcaggacttc catccttctt catgatctgt tcggcctttt
     2761 tcttattgcg caggatcggc tcgatctggg ccctcgcttt ggcgacagac atggtacctt
     2821 ccttatttat ctccgtgagc agaaccacta catacttatc accgacgggg aagggctcgg
     2881 agacattgcc cagatcggca tcataggccc agcgaaccag ttggcggttg atacccagcc
     2941 cggggatgga aaacgcggta ggcgcgatat cgggtgcaaa ctgtttttgc agatgctgtt
     3001 tttcggcatt ggcttcgaat gcctgtgcat tgcggctttg accggcaaac tggctcgcca
     3061 ggccggatgc ggcctgatcg gtctccgtac tggtttcgat cttgcggctc agctgagcga
     3121 tcttgtaggc tgtttcgaaa ttcttttgat cgaggatctc gatataatgg tatccatact
     3181 ggctcttcac gattttggtc tcgcccttct taccgt
//
LOCUS       DASYSV010000015         5821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_901278, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000015 DASYSV010000000
VERSION     DASYSV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901278"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /gene="dusB"
                     /locus_tag="VGQ51_00455"
     CDS             <1..944
                     /gene="dusB"
                     /locus_tag="VGQ51_00455"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV9035048.1"
                     /translation="NGADLMYSEFISSEGLIRDAIKSRQKLDFFEYERPFGIQIFGGD
                     EDAMAMSARIVDTVQPDLVDINFGCPVKKVVCKGAGAAVLKDVAAMTRLTTAVVKGTS
                     LPVTVKTRLGWDDASKNIGEVAERLQDAGISALTIHGRTRAQLYKGEADWTLIARVKE
                     NPRIRIPIFGNGDIDSPEKARAYRDRYGVDGMMIGRAAIGYPWIFNEIKHFLATGEHL
                     PPPTIGQRIGVIRDHLLRSVEWKGAVVGILEMRRHYSNYLKGFPHIKEFRNQLVVKAT
                     VEEIEEVLIQVERRYDGFVPERAFVQFSASQLEDCAW"
     gene            complement(984..2366)
                     /locus_tag="VGQ51_00460"
     CDS             complement(984..2366)
                     /locus_tag="VGQ51_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV9035049.1"
                     /translation="MSDPAVSSDISPLRHAFDSGVTRDYAWRRQQLLRLRQAIIDRED
                     TIAAALHTDLNKGREETYATETGLVLAEIRFLLKHLRSWMRPQSVGTNLVNLPAASRI
                     YHDPLGVILIVAPWNFPFQLSLLPLAAAIAAGNTVVLNPSEMAPATAAVIAGLLGEIF
                     PADMVRVIQGDGATIVPDLIRGFRFDHIFFTGSIPVGRSIYRMAAEQLVPVTLELGGK
                     SPVIVAADADAAVTARRIIVGKFANAGQTCIAPDYLLVHAEKSDELLHHLRRTLTGFY
                     GPHPENSPDYGRIINEKRFDTLVRYLSDGKIVHGGAHDRAARYIAPTLLTEVSPEAPV
                     MQDEIFGPLLPIFIWRTMEEALALIRRHPDPLAFYLFAKDKRLQRQWVDALPFGGGCI
                     NNTDWHFTNPHLPFGGIGNSGIGAYHGKYSLERFSHAKAVMRSYTFIDPRVKYPPFKG
                     KMKWFRIFIR"
     gene            2416..2862
                     /locus_tag="VGQ51_00465"
     CDS             2416..2862
                     /locus_tag="VGQ51_00465"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV9035050.1"
                     /translation="MNDEYFMRQALKEAQQAYEEDEVPIGAVIVMGGRIIARGRNQTE
                     RLNDCTAHAEIIALTSAFNHLGSKYIPEAAIYVTVEPCLMCAGALYWSKIGKVVFGAE
                     DEKNGYRRDVENASAPWPFHPKTQLVKGILGDECADLMREFFRSKR"
     gene            2998..3603
                     /locus_tag="VGQ51_00470"
     CDS             2998..3603
                     /locus_tag="VGQ51_00470"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV9035051.1"
                     /translation="MAFTLPALPYAFDALEPHIDATTMQIHHGKHHQAYVDNLNKAIA
                     GTPNENKSLEELVAKAGTISPAVRNNAGGHWNHSFFWEILSAKGGAPSGALSEAIDSA
                     FGNPDGLKEKMNAAGLSRFGSGWAWLIVKDGKLEVSSTPNQDNPLMDVAEVKGTPILG
                     VDVWEHAYYLKYQNKRGDYLKAFWSVVNWDKVAAHYSKAKK"
     gene            3652..4269
                     /locus_tag="VGQ51_00475"
     CDS             3652..4269
                     /locus_tag="VGQ51_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035052.1"
                     /translation="MRNYSLLLLVLAGLSACKGHEKKVLVYASDKISVDNTQHHITIA
                     NGDGTTHHEQELDFTSGDPVTLDVESAQGKYSVTIPDDGLYIANLKTDTVVGSRQHVG
                     AEGGESHISQDDLKKKLDSLNKLVADQNVSEANQNYFIAPHQAVKITSETKAKVFGPF
                     TSIPGAFDAGSVPAIYKFYSMSEMRELIANLQKMTGGSSDSTSKK"
     gene            complement(4272..4502)
                     /gene="yidD"
                     /locus_tag="VGQ51_00480"
     CDS             complement(4272..4502)
                     /gene="yidD"
                     /locus_tag="VGQ51_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV9035053.1"
                     /translation="MRKILRLLGLPLIALIRIYQWVISPMIGPRCRFNPSCSHYAVEA
                     LKKHGLFKGLWLSVRRIARCHPWGGHGIDPVP"
     gene            complement(4499..4921)
                     /gene="rnpA"
                     /locus_tag="VGQ51_00485"
     CDS             complement(4499..4921)
                     /gene="rnpA"
                     /locus_tag="VGQ51_00485"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV9035054.1"
                     /translation="MNPRNTLKKTERLKSRKQIETLFREGRSFSIFPFKVFYLLIPPM
                     PAAPPLQAAPPLQAAPPLQAGFGVSSRLFRKAVDRNRIKRLTREAWRLQRHPLSDHLG
                     KKGLSMAVFLIYTGKQLPELPLVTGKIGVALQKLIKQA"
     gene            complement(4998..5153)
                     /gene="rpmH"
                     /locus_tag="VGQ51_00490"
     CDS             complement(4998..5153)
                     /gene="rpmH"
                     /locus_tag="VGQ51_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV9035055.1"
                     /translation="MKRTFQPHRRRRKTVHGFRKRMQTANGRKVLASRRAKGRHKLTV
                     SDESKLK"
     gene            complement(5200..>5821)
                     /locus_tag="VGQ51_00495"
     CDS             complement(5200..>5821)
                     /locus_tag="VGQ51_00495"
                     /inference="COORDINATES: protein motif:HMM:NF039704.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HEV9035056.1"
                     /translation="ATYANIDSSFLYAFTTNEAPDTAQAGGQVTITEIDQTNQTISGT
                     FSFNAYRTVDGTQKTITQGVFTKIPYSSTQPTTTNGDTLTANIDARGWVGKSIQAAVN
                     AGELTIVGSSADGSQSVGLLMPANTTPGIYPLDGTNPSYLGVYTVLVNANTTGLVSVS
                     GTVTVTQNNTTTSRITGTFTFSAADPSNPTTVHYVTSGVFSVYYGP"
BASE COUNT         1339 a         1497 c         1728 g         1257 t
ORIGIN      
        1 agaacggggc cgatctcatg tacagcgaat tcatctcgag cgaagggctt atccgggatg
       61 cgataaagag ccggcagaag cttgattttt ttgaatacga acgacccttc ggtatccaga
      121 tcttcggagg tgatgaagac gcaatggcga tgagcgcccg tatcgtggat acggtgcaac
      181 ccgacctggt ggacattaac ttcggctgtc ccgtgaagaa ggtcgtgtgc aaaggggccg
      241 gcgctgccgt tttgaaggat gtggcagcaa tgacacggtt gaccacggca gtagtcaaag
      301 gcacttcact gcctgtgacg gtgaagaccc ggttgggatg ggacgacgct tcgaaaaata
      361 tcggggaggt tgccgagcgg ttacaggacg ccggtatcag cgcccttacc attcatggac
      421 gcacgagggc gcagctgtat aaaggagaag ccgactggac gctgatagcc cgcgtgaagg
      481 agaacccgcg gatcaggatc ccgatcttcg ggaatggaga tatcgactcg cccgaaaagg
      541 cccgtgcgta ccgggatcgt tatggcgtag acgggatgat gatcggcagg gccgccatcg
      601 gatatccctg gatcttcaat gagatcaaac acttcctggc gaccggggag catctgcctc
      661 cgcccaccat cggccagcgc atcggcgtca tccgcgatca cctgttgcgg tcagtggaat
      721 ggaaaggagc ggtcgtgggt atcctggaaa tgcggcggca ttattcgaat tatctcaagg
      781 gttttccgca tataaaagaa tttcgcaatc agctggtggt gaaggcaacg gtggaagaga
      841 tagaagaggt gttgatacag gtagagcggc ggtacgatgg attcgtcccc gaaagggcct
      901 tcgtccaatt ctccgcctct caattggagg attgcgcgtg gtaagaagcc gccccgcgat
      961 gctgtcgtct cattcgcgcg ccgctaccgg ataaagatcc tgaaccattt cattttcccc
     1021 ttgaacggcg ggtattttac gcgcggatcg ataaaggtat agctgcgcat caccgccttg
     1081 gcgtgcgaaa aacgctccag cgaatatttc ccgtgatagg cgccgatgcc gctgttaccg
     1141 atgccgccga agggcaggtg cgggttggtg aaatgccagt cggtattgtt gatacatcct
     1201 cctccgaaag gcagggcatc tacccattgc cgttgtaatc gtttgtcttt tgcgaaaagg
     1261 tagaaagcca gcggatcggg gtgacgccgg attagcgcca gcgcttcttc catagtgcgc
     1321 cagataaata tcggtaacag aggaccgaag atctcgtcct gcatcacggg tgcttcgggt
     1381 gatacctccg tcaggagggt aggcgcgata taccgggctg cgcggtcatg agctccgcca
     1441 tgcacgatct ttccgtcgga taggtagcgg accagcgtgt cgaaccgttt ttcattgatg
     1501 atccgcccat aatcggggct gttctccggg tgcgggccat aaaacccggt aagcgtccgt
     1561 cgcagatggt gtaacagctc gtcgcttttt tccgcatgaa cgagcaggta atccggagcg
     1621 atacaggtct gacccgcatt ggcgaatttt ccgacgatga tccgtctggc agtgacggcg
     1681 gcgtctgcat ctgcggccac gatgacggga ctcttaccgc ccagctcgag ggttacgggg
     1741 accagctgct ccgccgccat gcggtagatc gacctgccta ccgggatgct tccagtgaaa
     1801 aagatatgat cgaaacggaa tccccggatg agatcgggga cgatcgttgc gccgtcaccc
     1861 tggatgaccc ggaccatatc ggcagggaag atctcgccga gcagaccggc gatcacggcg
     1921 gccgtagcgg gcgccatttc ggatggattg agcacgacgg tatttcctgc agcgatggca
     1981 gccgccagcg ggaggagcga cagctggaaa ggaaaattcc acggtgcgac gatcaggatc
     2041 acacccagtg ggtcgtgata aatgcggctt gcagccggaa ggttgacgag attggttccc
     2101 acgctttgcg gccgcatcca gctacgcagg tgtttcagaa ggaagcggat ctcagccagc
     2161 accaggcccg tttccgtggc ataggtttcc tccctgccct tattcagatc ggtatgcagc
     2221 gccgctgcta tcgtatcttc gcggtcgatg atcgcctgac gcaggcgcag gagttgttgc
     2281 cgccgccagg catagtcgcg cgttacgccg gagtcgaagg cgtggcgaag tggggaaatg
     2341 tcggaggaga cagcggggtc gctcatattc caatttccaa agaatttccg gtatttacac
     2401 gatatttttg tggtaatgaa cgacgaatat tttatgcggc aggccctcaa agaggcgcag
     2461 caggcctacg aagaggacga ggtgccgatc ggggccgtca tcgtcatggg tgggcgtatc
     2521 atcgcgagag gccgtaacca gaccgagcgg ctgaatgact gcactgcgca tgccgagatc
     2581 attgccctga cctcggcctt caatcacctc ggcagcaaat atattccgga agccgcgatc
     2641 tatgtcaccg tggagccctg tctcatgtgc gccggcgcgc tttactggag caagatcggg
     2701 aaggtggtat tcggtgctga agatgagaag aacgggtatc gccgggacgt cgaaaacgct
     2761 agcgcgccgt ggccctttca cccgaagacc caattggtga agggcatcct gggggatgaa
     2821 tgcgcagacc tgatgcggga gttcttccga agcaaacgct gagcctcacc tgtcgcccgg
     2881 caaggcggtc gcttgggagc attcctttgt cttctgggca aattcctgta gatttgctat
     2941 cccttcaggc attgacacga cggtaatatt ttatttaata tcattaaaac attcgttatg
     3001 gcatttacac ttcctgcgct gccttatgca ttcgatgcat tggagccgca catcgacgct
     3061 acgaccatgc aaatccatca tggcaagcat caccaggcct atgtcgacaa tctgaacaaa
     3121 gcaatagcgg gtacgccgaa tgaaaataaa tctctcgaag aactggtcgc aaaggcgggc
     3181 acgatcagcc ctgccgttcg caacaatgca ggcggtcact ggaaccatag ctttttctgg
     3241 gagatattga gcgcaaaggg cggagccccg tcgggcgcat tgtcggaagc aatcgacagc
     3301 gccttcggca atcccgacgg cctgaaggag aagatgaatg ccgccggtct atcccgtttt
     3361 ggtagcggct gggcctggtt gatcgtgaag gatggcaaac tggaggtctc ttcgaccccc
     3421 aaccaggaca acccactgat ggatgtagcc gaagtaaaag gtactcccat cctgggcgtc
     3481 gacgtttggg aacatgccta ttacctgaaa taccagaaca aacgcggaga ttatctgaag
     3541 gcgttctgga gtgtggtcaa ctgggataag gtagcggctc attattcgaa agcgaaaaaa
     3601 taggattatt tcacaaccgg ctctttacgg agccggtttt taacaactac tatgagaaat
     3661 tacagtctcc tgctcctcgt ccttgccggt ctgtcggctt gtaaaggtca tgaaaaaaaa
     3721 gtgctggtct atgccagcga taagatctcg gtagataaca cccaacatca tatcaccatc
     3781 gccaacgggg acggtaccac gcatcatgag caggaactgg atttcacctc cggcgacccg
     3841 gttacgctgg atgtggaatc cgcacagggc aagtatagcg tcacgatccc cgacgacggg
     3901 ctctatatcg ccaatctgaa aacggatacg gtcgtcggca gccgtcagca tgtcggagcg
     3961 gaaggtgggg agagccatat ttcccaggac gatctgaaaa agaagctgga cagtctgaac
     4021 aagctggtcg cggatcagaa cgtcagcgaa gccaatcaga attattttat cgctccgcat
     4081 caggcggtta agatcacttc ggaaacgaag gcaaaggtct tcgggccgtt tacgagcata
     4141 ccgggggcgt tcgatgcagg aagcgtgccg gccatttaca aattctacag catgtcggaa
     4201 atgcgggaac tgatcgccaa cctgcagaag atgacaggcg ggtcttccga ctctacttca
     4261 aaaaagtagc gtcagggtac cggatctatc ccatgtccgc cccagggatg acagcgggcg
     4321 atgcgacgaa cgcttagcca taggcccttg aacaacccat gctttttcag cgcctcgacg
     4381 gcatagtgtg agcagctcgg attgaatcgg caacgcgggc cgatcatcgg ggagatcacc
     4441 cattgataga tcctgatcag tgcgatcagc ggcaacccca acaatctcag aatcttcctc
     4501 atgcctgctt gattaatttc tgcaaagcta cgccgatctt cccggttacg agcggaagtt
     4561 cgggcagctg tttgcccgtg tagataagga ataccgccat agaaagccct ttttttccca
     4621 gatggtcgga cagtgggtga cgctggagac gccaggcctc gcgggtgagg cgtttgatgc
     4681 ggttgcgatc gaccgcttta cgaaagagcc gggaagagac cccgaaaccg gcttgcaggg
     4741 gcggggccgc ttgcaggggt ggggctgctt gcaggggcgg ggcggcgggc atcggtggta
     4801 tcagcaaata gaagaccttg aagggaaaaa tggagaaact tcgcccctcc cggaacaacg
     4861 tctcgatctg tttccggctc ttgagccgct cggttttttt gagtgtattt cgcggattca
     4921 ttcgacctcc ttacgaaaaa tagcttcggg atacccgaag ctattcaata aaaaaattat
     4981 atctggtacc gaagggtcta tttcaatttg gactcatcgg aaacggtcag cttatgacga
     5041 ccttttgccc ggcggctggc caggacctta cggccattgg ccgtctgcat cctcttgcgg
     5101 aaaccatgca ccgtctttcg acgacgtctg tgtggttgaa atgtacgctt catgaataat
     5161 caattttacg ggactgcaaa ggtaagggat ttttttcaat tatggcccat aatataccga
     5221 aaataccccg ctggtgacat aatgtaccgt ggtggggttg ctgggatcgg cggccgaaaa
     5281 ggtaaaggta ccggtgatcc ggctggtggt cgtattgttc tgggtcacgg tgaccgtgcc
     5341 ggaaacagac accaggccgg tcgtattggc attcaccagc accgtataga ccccgagata
     5401 ggatgggttc gtgccgtcca ggggatagat gcccggagtg gtattggcag gcatgagcag
     5461 cccgaccgac tggctgccat cggcagacga gcctacgata gtcagttcgc ccgcgttgac
     5521 ggccgcctgg atacttttcc cgacccaacc cctggcgtcg atgttggcgg tcagggtgtc
     5581 cccgttagtg gtcgtaggct gggtcgaact ataaggtatt ttagtaaaaa cgccctgagt
     5641 gatcgtcttc tgcgttccgt ccacggtgcg gtaggcatta aaagagaagg tgccgctgat
     5701 cgtctgattg gtctggtcta tttcggtaat ggtgacctgg ccccccgcct gagcggtatc
     5761 gggcgcctca ttggtcgtaa aggcgtacaa aaaggaagaa tcgatgttgg cataggtggc
     5821 c
//
LOCUS       DASYSV010000016         4083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2181411, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000016 DASYSV010000000
VERSION     DASYSV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181411"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VGQ51_00500"
     CDS             complement(<1..1009)
                     /locus_tag="VGQ51_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEV9035057.1"
                     /translation="MTRNILTGLISGAACCLTIAAHAQTRFADRTLKSADRQYRYLMK
                     QVPEGVMPRSFQHDSLRTCTSENWVAGFYPGTLLEIYEGTKDHVLYDEALRKLPLMDS
                     QQYNSGTHDLGFMLFCSYGTLYRLAPDAKTKQLLLNAARSLSTRFNPRVGCIRSWGKS
                     GDTTSFRVIIDNMMNLELLLWATHVTGDSSFYRIAVTHANTTMRNHFRPDYSSYHVVV
                     YNPQTGAVMKKQTAQGASDESAWARGQSWGLYGYTMLYRYTHDPRYLDQAQHIAAFLL
                     NNPNLPADKVPYWDYNAPGIPNVERDASAGAVMASALIELSGYSKATLATRYLSTADT
                     IL"
     gene            complement(1018..3603)
                     /locus_tag="VGQ51_00505"
     CDS             complement(1018..3603)
                     /locus_tag="VGQ51_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEV9035058.1"
                     /translation="MNRLLTTSLLCTLLAGQTALAQSPGRTSFTIDDGWKYIPEGMAY
                     AQDPKRNDIAGQEVSLPHTWNATDVFDDDRTYKRSIGWYRKQLHFGPEYSGKRIFLWF
                     EGAGQVADVYVNGDFLGRHKGGYTGFGFDITDRLKHNVDGSSEGLVAVQVNNAHDPFV
                     PPLSIGYASYGGIYRDVWVITTGSVHFTDVNNNAGGVFISTPDVATGKGTVAIRSVIS
                     NETDRPAAITFVNEICDSAGRPIRSTKVDYSVGPHQRVPVAASVDAGNEPRLWSPSDP
                     YLYKLKSHIEIDGKTIDEVDNSLGFRWFSFSPDKGFSLNGRKLVLHGTTRHQDMLGKG
                     DALSAEDHERDLRMIKNMGANFIRLAHYPQAPKVLQLADELGLIIWEEVPVVNYMTLD
                     DEFFHNAQNMTREMIRQGYNHPSVVMWGSMNEILLYSKDGVRIQKHTDTAYIGALKRY
                     AVRLDSTIRAEDPTRYSTMAMHLSDDYAKFGIDRISQVAGHNIYDGWYSGALEDFGRD
                     VDAIHHNEPQQNIFISEYGAEGEVRLNTERPGRMDYTEEYQRLYHESYLRQINARPFL
                     AGTSIWNEFDFSQPNIGGPQGHRNQKGLVTWDRQPKDVYYLYKANWNPAPMVYIASRN
                     WAVRAGEAHALNTIDAYSNLPQVTLTVNGIDQGARKPDDIHKARWSVQLANGKNAIRV
                     TGHSGNSTISDEMTIDYRAYDAKPAAPLSINVGSNAQYLDAPGTVWIEDREYKPGGFG
                     HIGGNPGLFARGEIIKGTADQPLYYSYLDSLQGYRFDVKDGKYNVTLYFAESKDLLPG
                     QRVFDVTINGQPVEHGLDLAASYGYAEAIKKTYIVEASDGQGIRIVFTAKKGNTLLSG
                     IKIEQ"
     gene            complement(3618..>4083)
                     /locus_tag="VGQ51_00510"
     CDS             complement(3618..>4083)
                     /locus_tag="VGQ51_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9035059.1"
                     /translation="DSTGKEKLSNPNWPSTSKWDWSSPTNVLIDPQYNSQFILRTAET
                     YLLLAEADYDLGDMQGAADAINALRARAHAAPVTAADITIDLILDERARELFSEEDRR
                     YVLLRTGRWLDRVKADNVIAAPGVQARDTLLPIPQPVIDANRTKPMTQNPGY"
BASE COUNT          776 a         1113 c         1220 g          974 t
ORIGIN      
        1 gcaggatggt atcggccgtg gagaggtatc gcgtcgcaag cgttgctttg ctatatcccg
       61 aaagctcgat cagtgccgaa gccatgaccg ctcccgcaga tgcatcgcgc tccacgttag
      121 ggatgccggg cgcattgtaa tcccaatacg ggaccttatc ggccggcaga ttgggattgt
      181 tgagcaggaa ggcggcgata tgctgcgcct gatccaaata gcgcgggtca tgggtgtagc
      241 ggtataacat cgtatagccg tatagtcccc agctctgtcc ccgcgcccag gccgattcgt
      301 cggacgctcc ctgcgccgtt tgtttcttca tcacggcgcc tgtctgcggg ttatatacca
      361 cgacgtgata tgagctatag tcgggccgga aatggttgcg catcgtcgta ttcgcatggg
      421 tgaccgcaat gcgatagaag gaagaatcgc ccgtgacatg cgtggcccat aacagcagct
      481 cgagattcat catattatcg atgatgaccc ggaaggaggt ggtatcaccc gactttcccc
      541 agctgcggat gcagcctacc ctgggattga agcgcgtcga cagcgagcgg gccgcattga
      601 gcagcagctg ttttgtcttt gcatccggtg ccagccggta gagcgttcca tagctgcaaa
      661 agagcatgaa cccaaggtca tgagtgccgc tgttgtattg ctggctgtcc atgagcggca
      721 gcttgcgtag ggcctcatcg tagaggacgt ggtctttcgt gccttcatag atctccaaca
      781 gtgtgccggg atagaagccg gccacccagt tctcagaagt gcaggtgcgg agactgtcat
      841 gttgaaagct gcggggcatg acgccttcgg gcacctgttt catgaggtac cgatattgcc
      901 tgtcggcgga tttaagcgtc ctgtcggcga aacgcgtctg agcgtgagcg gcaatcgtca
      961 ggcagcaggc ggcgccggag atcaatccgg tgagaatgtt tcttgtcatg gttcctttta
     1021 ttgttcgatc tttattccac tgagcagcgt gttccctttt ttagccgtga agacgatgcg
     1081 gatgccctgg ccgtcgctgg cctccacgat atacgtcttt ttgatggcct cggcatagcc
     1141 atagctggcg gccaggtcaa gaccgtgttc tacgggctgt ccgttgatgg tgacatcgaa
     1201 gacccgctgc cccggtaaaa ggtctttgct ctcggcaaaa tagagtgtca cgttatactt
     1261 cccatccttt acgtcgaacc ggtagccctg cagactgtcg aggtacgaat aatacagcgg
     1321 ttgatcggcc gtgcccttga tgatctcgcc gcgggcgaac aggccggggt taccgccgat
     1381 atgtccgaag ccgccgggct tgtattccct gtcctctatc cataccgtac ccggggcgtc
     1441 caggtattgg gcattggagc ccacgttgat ggacaaaggc gccgcgggtt tggcgtcata
     1501 tgccctatag tcgatggtca tctcatcgga gatggtgcta ttgccggaat ggccggtgac
     1561 gcggatggcg ttctttccgt tagccagttg aacggaccag cgcgccttat gtatgtcgtc
     1621 gggcttgcgg gcgccctggt cgatgccgtt aacggtgagg gtcacctgtg gaagattgct
     1681 gtatgcgtcg atggtgttga gtgcgtgtgc ttcgcccgcc cttaccgccc agttacggct
     1741 ggcgatgtat accatgggtg ccgggttcca attggcctta tacaaataat agacatcttt
     1801 cggctgtcgg tcccaggtga ccaggccttt ctggttgcga tggccttgcg gaccgccgat
     1861 attgggctgc gagaaatcga actcattcca gatggaagtg ccggccagga agggtcgtgc
     1921 gtttatctgc cggagatagc tctcatgata gagccgctga tattcctccg tataatccat
     1981 tcgcccgggt ctttccgtat tgagacgtac ttccccttct gcgccgtatt cactgatgaa
     2041 gatgttctgc tgcggctcgt tatggtgaat ggcgtcgaca tccctgccga agtcttccag
     2101 agcacccgaa taccagccgt catagatgtt atgcccggct acctgcgaga tgcggtcgat
     2161 gccgaacttc gcatagtcgt cgctgaggtg catcgccatc gtgctgtaac gcgtcgggtc
     2221 ttctgcgcga atggtactat ccaggcggac ggcatagcgt ttcagcgcgc cgatataggc
     2281 ggtgtccgta tgtttctgaa tgcgaacccc gtctttgctg tagagcagga tctcgttcat
     2341 ggacccccac atcacgaccg acggatggtt atatccctgg cggatcatct cgcgggtcat
     2401 gttctgcgcg ttgtgaaaga attcgtcgtc gagggtcata tagttcacga ccggcacctc
     2461 ttcccagatg atcagtccca gctcgtcggc gagctgcagg actttcggcg cctgcggata
     2521 gtgcgccagg cgaatgaaat tggcgcccat gttctttatc atccgaagat cgcgttcatg
     2581 gtcttcggcg ctcagcgcat ctcccttgcc cagcatatcc tggtgtcgcg tcgtgccatg
     2641 cagtacaagt tttcgtccgt tgaggctgaa gcctttatcc ggactgaagg agaaccagcg
     2701 gaagcctaag gagttatcca cctcgtcgat ggtcttgcca tcgatctcga tatggctttt
     2761 caacttatat aggtaaggat cggacgggct ccagagccgc ggttcgttac cggcatccac
     2821 agacgcggcg acgggcaccc gctgatgcgg tcctacggaa tagtcgactt tcgtactgcg
     2881 tatcggtctg ccggcggaat cgcagatctc attcacgaag gtaatggcgg cggggcggtc
     2941 ggtctcgttg gagatgacgc ttcggatagc caccgttccc ttgcctgtgg cgacatccgg
     3001 agtgctgatg aatactccgc ccgcgttgtt attgacatcc gtaaaatgca cggaacccgt
     3061 cgtgatcacc caaacgtccc ggtaaatacc gccataggaa gcatacccga tggataaagg
     3121 aggcacgaac ggatcatgag cattgttgac ctgtacggcg acgaggccct cactgctgcc
     3181 gtcgacgttg tgtttcagcc ggtcggtgat atcgaaaccg aaacccgtgt agcctccctt
     3241 atgccggccc aggaaatcgc cattcacata cacatcggcg acctggcctg ctccttcgaa
     3301 ccacaggaag atacgcttcc ctgaatattc aggcccgaag tgaagctgtt tcctgtacca
     3361 cccgatgctg cgtttgtagg tgcggtcatc gtcgaagaca tcggtggcat tccaggtatg
     3421 cgggagagag acctcctgac cggcaatatc attccgcttc ggatcctgcg cataggccat
     3481 cccttcggga atgtatttcc agccgtcgtc gatggtgaag ctggtcctgc ccggcgactg
     3541 tgcgagcgcg gtttgcccgg cgagcagggt gcaaagaagg cttgttgtca gtagtctgtt
     3601 catgttgcga agtgcgatca atagccagga ttttgggtca tcggtttggt gcggttggca
     3661 tcgatcaccg gttgaggtat cggcagcagg gtatcccggg cctgtacgcc cggcgcggcg
     3721 atcacgttgt ccgcttttac cctgtccagc catcggccgg tgcggagcag gacatagcgt
     3781 ctgtcttctt cggagaagag ctcacgggcg cgttcgtcga ggatgagatc gatggtgata
     3841 tcagccgccg tcacaggagc cgcatgggcc cttgcacgga gcgcgttgat cgcatcggcc
     3901 gcgccctgca tatcgccaag gtcataatcg gcctccgcca gcaacagata ggtctctgcc
     3961 gtacggagga tgaactggga attgtattgc ggatcgatca ggacgttggt ggggctgctc
     4021 cagtcccatt tggaggtgga cggccagttc gggttggaca gtttctcctt gccggtgctg
     4081 tcg
//
LOCUS       DASYSV010000017         6536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2310911, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000017 DASYSV010000000
VERSION     DASYSV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310911"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VGQ51_00515"
     CDS             complement(<1..1209)
                     /locus_tag="VGQ51_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV9035060.1"
                     /translation="MATEWQGKLQTVGAIFARSYEHIKSIGFTIHMEDYEKRKLGIFN
                     QLNFFQLLTGTLIPLAGLISNRHFHTSAWIVACMPAVISILVLAFNARREYYLAQIAY
                     FVVNPIAISFVYFWGFDMGVELSFILYGILSVFFIQDLSQMLFALALSMASYFGLAVL
                     CRNYTYQLATVSPFLYMFNQLLAIGFIFVGLFLIKKENAGYQQSILKQKEAIAANEKL
                     LRQQTDELTQINSFKNRLFSIIAHDLKSPIYALRNLFRNMQQLDMPADDIKELVPDVV
                     NELTHTTSLMENVLHWARSQMQAYTVKPQPIGVPDLIAEVARLLRLQADAKQIGVTLK
                     SEASVVAFADRDMVHLVLRNLLSNAIKYTPENGCIEMGARREDGAIALFVKDTGTGIP
                     QNDLEKIQLNN"
     gene            complement(1454..2221)
                     /locus_tag="VGQ51_00520"
     CDS             complement(1454..2221)
                     /locus_tag="VGQ51_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEV9035061.1"
                     /translation="MNFNLTQIPERTTRPRTYGLTMINDKGLSVGEARNLVSGASPHV
                     DLVKLAFGTSLVSAGLKEKIRVFHDAGIPVYFGGLLFEAFVIRHQFDDYISLLKEYDI
                     TWIEVSDGSIDITHSEKCGYIEKLSRYGTVLSEVGSKDKDREHITPPYQWIRLMQAEL
                     NAGASYIIAEARETGNVGLYRDSGEVREGLVQEILTKVPAEKILWEAPQKDQQLYFLT
                     LCGANANLGNINPAEVIALEAMRLGLRGDSFELFLKK"
     gene            2338..2622
                     /locus_tag="VGQ51_00525"
     CDS             2338..2622
                     /locus_tag="VGQ51_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV9035062.1"
                     /translation="MAQMDAKQAEKISKALSDPTRLKILSEIKKKHDWLYCVDLYENI
                     HLAQPSICHHLKQLTETSIILPEKEGRNIKYTMNNEVIDEYIGFLQGLKK"
     gene            2955..5372
                     /locus_tag="VGQ51_00530"
     CDS             2955..5372
                     /locus_tag="VGQ51_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HEV9035063.1"
                     /translation="MPDTRRDFLKKAALLTGGVSLHPLLPQRIQRALAIDPAAGSTWL
                     DAEHVVILMQENRSFDHALGTLRGVRGYNDPRAIQLPNGNPVWLQSNTEGGTYAPFRL
                     DIRNTRATWMDSLPHSWSNQVLARNDGRYDQWLNVKRNSIKEYSNMPLTLGYHTREDL
                     PFYYALADAFTVCDQNFCSSLTGTNPNRLYFWTGTVREEQHEGSRAHVYNDDMDYGTL
                     KWKTFPERLQALGIPWKCYQNEVAIDTGFEGEEDRWLSNFQDNPLEFFAQYNIRLYPR
                     HIDAMKAKKPGHVPNQTLHDLPKEAQDLHRRAFDSNAGDPQFRSLEELLYEDKGIHRT
                     MKAPKGDVLHQFREDVRSGKLPMVSWLSAPENFSDHPSAPWYGAWYVSEVLDILTQNP
                     EVWKKTVFILAYDENDGYFDHVPPFTAPDPHRPGKGKVSEGIDTRVDFITKEQARERK
                     GFPERFERECSIGLGFRVPLIIASPWSRGGWVNSQVFDHTSTLQLLEKFLSHKTGREV
                     REPNISDWRRLVCGDLSSVFRPYFGERIAEPTSVPRELFLESINSAQYRKLPDTFKAL
                     TPAEIALFKQEPRNSPYATRQEPGTRPSCALPYCMLVDGRLNADRRSFGIRFENSNLA
                     FGGRTAGAPFNVYAPGEYAAADNPGHFEALRTWAYAVRPGDTLSDKWPLADFQGGRYH
                     LRVYGPNGFYREFGGDANDPALEIVFAYRPLMERGQVFDEVGVLAWKNSGKACMLELI
                     DHSYGSVYPGRSLAAGEKNSMEIPLAASHGWYDFSVRVHGHPAFGIRYAGHIETGMDS
                     VSDPAMA"
     gene            complement(5376..6113)
                     /locus_tag="VGQ51_00535"
     CDS             complement(5376..6113)
                     /locus_tag="VGQ51_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV9035064.1"
                     /translation="MPKEKNYKWKLGLFSLAALAIAIAGIYYVGKQKNKFGTVLHLSS
                     RFNSVVGLKIGSNVRVGGIDVGTVDGISLVTDTTVQVDMIIQEKIQKFIRRDAKASIG
                     SDGLMGDKVVIINAGTPASPLVADGDSLGSRQPIETDAIMSSLKISADNAAVITSNLA
                     DISSRISQGHGALGRLLHDTTLSANISATVKNLKSGSKKLDENMEAAQHNFLLRGFFK
                     KKKKKQEEAKAAQEQVQQQKTNKENKP"
     gene            complement(6125..>6536)
                     /locus_tag="VGQ51_00540"
     CDS             complement(6125..>6536)
                     /locus_tag="VGQ51_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9035065.1"
                     /translation="RQHKKTMSKEKKEALINEMLDNVGLLDSIDLLPSKLSGGQSKRI
                     GLARTLILRPEIMLYDEPTTGLDTGTAREISDLICQMKRKYNISSITITHDMPCARIT
                     ADRIILLKDGIVYAEGSYEDLEQSEDEWIRSFFY"
BASE COUNT         1485 a         1647 c         1834 g         1570 t
ORIGIN      
        1 attattgagc tgtatctttt cgaggtcgtt ctgaggaata ccggtaccgg tgtctttcac
       61 gaacagggct atggcgccat cttccctccg ggcgcccatc tcgatacacc cattctcggg
      121 cgtgtatttg atcgcattcg aaagaaggtt tcgaagaacg agatgtacca tatcccggtc
      181 ggcgaacgcc accacggaag cttccgattt cagcgtaacg ccgatctgtt tggcgtccgc
      241 ctgcagccgg agcaaccgtg ctacttcagc aatgagatcg gggacgccga taggctgtgg
      301 tttgacggta taggcctgca tctggctacg cgcccaatgc aatacgttct ccataaggct
      361 ggtggtatgc gtcagctcgt tgacaacgtc cggaaccagt tcttttatat cgtcggcggg
      421 catgtccagc tgctgcatat tgcgaaagag attgcggaga gcgtagatcg gggatttcag
      481 gtcatgggcg atgatggaaa aaagccggtt cttgaaggag ttgatctgcg tcagctcgtc
      541 ggtctgttgc cggaggagct tttcattcgc ggcgatggct tccttctgtt tcaggatgct
      601 ttgctgataa ccggcattct cttttttgat caggaaaaga ccgacgaaga tgaacccgat
      661 ggccagcagc tggttgaaca tatagaggaa ggggctgacc gtagccagtt gataggtata
      721 gttcctgcac aggaccgcca acccgaaata gctggccatc gatagcgcaa gcgcaaacag
      781 catctggctt agatcctgga taaagaagac ggataagatg ccatagagaa tgaaggaaag
      841 ctccactccc atgtcgaatc cccagaaata aacaaagctg atcgcgatgg ggtttacgac
      901 gaaataggcg atctgggcga gatagtactc acggcgtgca ttgaacgcga ggacgagtat
      961 gctgatgacc gccggcatgc aggcgacgat ccaggcggag gtatgaaaat gacggttgct
     1021 gatgaggccg gcgaggggaa tgagcgttcc ggtgagcaat tggaaaaaat tcagctggtt
     1081 gaagatgccc agctttcttt tttcatagtc ctccatgtgt atggtgaaac ctatagactt
     1141 gatgtgctcg taggacctcg cgaaaatggc accgacggtt tgaagcttgc cctgccattc
     1201 ggtagccata ccgaatggtt tgaattgttt gacgcgagca gtaagaaaca gaggtccaag
     1261 gattttcatt cagggcgatt cgggtttata cttggaaagc gtccggccaa aatagccctt
     1321 tttttcgata aaggcaatag ctatccgaag aaagctcccg ggtcatcgaa tccggccggg
     1381 ccggaacggt tattgcagac ttaggctata accatttgca tatcagctta ttcggaaagg
     1441 aaatcgagta tttttatttt ttcaggaata attcgaagga atctccgcgg agaccgagac
     1501 gcatggcctc gagtgcgatg acctcggccg ggttgatatt tcccaggttg gcattcgccc
     1561 cgcagagcgt caggaaatac agctgctgat ctttttgcgg cgcctcccag agtatttttt
     1621 cggccggcac tttcgtcagg atctcctgca cgaggccttc gcgtacctcg ccggaatcgc
     1681 gatagagacc gacattcccg gtttcgcgtg cttccgcaat gatataggaa gcccctgcgt
     1741 tcaattcggc ctgcatgagc cgtatccatt gatagggcgg cgtgatatgt tcgcggtcct
     1801 tgtcttttga acccacctcg ctgagcaccg tgccataccg ggataatttt tcgatataac
     1861 cgcatttctc ggaatgcgta atatcgatag agccgtcgga cacttctatc caggtgatat
     1921 cgtattcttt caggaggctg atataatcgt cgaactggtg ccggatgacg aaggcttcga
     1981 acaggagtcc gccgaaatag accgggatgc cggcatcgtg gaagaccctg atcttttcct
     2041 tcagaccggc ggacaccagc gaagtgccga aggccagttt gacgagatcg acatgcgggg
     2101 acgccccgga gaccaggttg cgagcctcac cgacactgag ccctttgtca ttgatcatgg
     2161 tgaggccata ggtccggggg cgtgtcgtcc tttcgggtat ttgcgtcagg ttgaagttca
     2221 tggtcgcaag ttagtgagac gaggcgaaat cgcgaaaaac ggcagttccg catgcgggga
     2281 gcctggccgc ggccgactgc ggtattttcc gtatcttaga tacaaatagt ttgtcatatg
     2341 gcgcagatgg atgcgaaaca ggcggaaaag atttccaaag ccctttccga tccgaccagg
     2401 ctgaagatcc tcagcgagat caaaaaaaag catgactggc tgtattgcgt cgacctttat
     2461 gaaaatatcc atctggccca accttccatt tgccatcatc tcaagcaatt gaccgagacc
     2521 tccatcatcc tgcccgaaaa agaaggaaga aatatcaagt acacgatgaa caacgaagtg
     2581 atcgacgagt atatcgggtt tttacaaggg ttgaagaagt aaaaaaagca gggattgccg
     2641 cagtaaaaaa acattgacga agttcgatgc ttttcccgta tatttgaaca ggatttgaca
     2701 gatcgaagaa tttcaatgta ttacctaaaa gaatgtgtta tgaatcacta ttgcattaac
     2761 cgcgaggata gtttttcctc gccccttctc aaagagagga ataaaagcct cggccggaat
     2821 ttcctttata ccgtgctcgt attcgttgcc ctgtgcgcaa tctcctattt gatggccaaa
     2881 tagaatggtt gccgggtctt cattttaatt atttcataat ctctgcttgc cgttgtttgg
     2941 catagatttg tactatgccg gatacgagaa gagatttcct gaaaaaagcc gccctgctga
     3001 cggggggcgt aagtcttcat ccattattgc cccagcgcat tcaaagagcg ctggccatcg
     3061 accctgccgc gggcagcacc tggctcgatg ccgaacatgt cgtcatttta atgcaggaga
     3121 acaggtcgtt cgaccacgcg ctgggaacgt tgcgcggagt ccgcggctac aatgacccgc
     3181 gtgccataca acttccgaac ggtaacccgg tatggctgca atccaacaca gaagggggca
     3241 cctatgcgcc attccgtctc gacatccgga atactagggc gacgtggatg gattcgttgc
     3301 cgcattcctg gtccaaccag gtcctcgcgc gcaacgatgg ccggtatgac caatggctca
     3361 atgtaaaacg caacagtata aaggagtatt cgaatatgcc gttgaccctg ggctatcata
     3421 cccgggagga cctgcctttc tattatgccc tggccgacgc cttcacggtt tgcgatcaga
     3481 acttctgttc gtctcttacg ggtaccaatc cgaaccggct ctatttctgg accggaacgg
     3541 tccgtgaaga gcagcacgag gggtcgcggg cgcatgtgta caacgacgat atggactatg
     3601 gcacactcaa atggaagacc ttccccgagc gattgcaagc gttgggcatc ccgtggaaat
     3661 gttaccagaa cgaagtggct atcgacaccg gcttcgaggg cgaggaggac cgctggctct
     3721 ccaatttcca ggacaacccg ctggaattct tcgcacaata taacatccgt ctctatccgc
     3781 ggcatatcga cgcgatgaag gcgaaaaagc cgggccatgt tccaaaccag acattgcatg
     3841 atctgccgaa ggaggcgcag gacctgcacc ggcgggcctt cgattcgaat gccggcgacc
     3901 cgcagtttcg ttcgttggaa gaactgcttt atgaagacaa gggcatacat cgtacgatga
     3961 aggccccgaa aggggatgtg ctccaccagt tcagggagga tgtccggtcg ggaaagctgc
     4021 ctatggtgag ctggctctcg gctccggaaa atttctccga ccatcccagc gcgccctggt
     4081 atggcgcctg gtatgtatcg gaagtactgg acatcctgac gcagaacccg gaggtgtgga
     4141 aaaagaccgt ctttatcctg gcttatgacg agaatgacgg ttatttcgat catgtgcctc
     4201 cttttacggc cccggatccg catcgcccgg gtaagggaaa agtctcggaa ggtatcgata
     4261 cgcgggtaga cttcataacg aaggaacagg cccgggagcg gaagggattt cccgaaaggt
     4321 tcgaacgcga gtgttcgatc ggacttggtt tccgggtgcc gctgatcatc gcatcgccat
     4381 ggagtcgcgg cggatgggtc aattcgcagg ttttcgatca tacgtcgacg ttgcaactgc
     4441 tggaaaaatt cctctcgcat aagacgggcc gtgaagtccg tgagccgaac atttccgatt
     4501 ggcgacggct ggtctgtggg gacctcagct cggtgttccg gccttatttc ggagagcgta
     4561 tcgcagaacc aacgtccgtg ccgagggagc tttttttaga aagtatcaat agtgcgcaat
     4621 accgcaagct tcccgatacg ttcaaagccc tgacgccggc ggagatagcg cttttcaaac
     4681 aggagccgcg caactcgccc tatgcgaccc gccaggagcc gggaacgcgg ccatcctgcg
     4741 cgttgcctta ttgtatgctg gttgacgggc ggctgaatgc ggacaggcgt tcgttcggga
     4801 tccgttttga aaattccaat cttgcattcg gtggacggac ggccggggct ccattcaacg
     4861 tatatgcacc cggggagtat gcggcggcgg ataatccggg ccatttcgag gccttgcgga
     4921 catgggcata cgcggtgagg ccgggagata cgctttccga caaatggccg ttagccgact
     4981 ttcagggagg acgataccac ctgcgggttt atgggccaaa cgggttctat cgggagttcg
     5041 ggggcgatgc gaacgacccg gcgcttgaaa ttgtcttcgc ctatcgaccg ctaatggagc
     5101 ggggccaggt attcgatgag gtgggtgtcc tggcctggaa aaatagcggg aaagcctgca
     5161 tgctggagct aatcgatcat tcctatggca gtgtttatcc cggcaggagt ttggccgcgg
     5221 gcgagaaaaa ttccatggag ataccactgg cagccagtca tggatggtac gacttcagcg
     5281 tccgggtaca tgggcatccg gctttcggga tcaggtatgc gggccatata gagacgggta
     5341 tggatagcgt cagcgacccg gcgatggcgt gatcattacg gcttattctc cttgttggtc
     5401 ttctgctgtt gcacctgctc ctgggccgcc tttgcctcct cttgcttctt tttcttcttt
     5461 ttgaagaacc cgcggagcag gaagttatgc tgggccgctt ccatgttctc atcgagcttc
     5521 ttgcttcctg acttgagatt ctttacggtg gcgctgatat tggcggaaag cgtagtatcg
     5581 tgcaggagcc ggcccagcgc gccatgaccc tggctgatac ggctggagat atcggctaaa
     5641 ttggacgtta tgaccgcggc gttgtccgcg gaaattttca gactgctcat gatggcgtcg
     5701 gtctcgatgg gttggcggga acccagcgag tcgccatccg cgaccagcgg ggaagccgga
     5761 gtacccgcat tgataattac gaccttgtcg cccatcagcc catccgatcc gatacttgcc
     5821 tttgcatccc tccggatgaa tttctggatc ttttcctgga tgatcatgtc gacctggacc
     5881 gtcgtgtccg tcaccagcga gataccgtct accgtcccga catcgatacc gccgaccctg
     5941 acattactgc caatcttgag accgacgacc gaattgaacc ggctgctgag atgcagcaca
     6001 gtgccgaatt tatttttttg cttaccgaca taatagatgc cggcgatggc tattgccagg
     6061 gctgctaaag aaaagagccc cagtttccat ttatagtttt tttcctttgg catagcgtta
     6121 gattttaata aaaaaacgat cgtatccatt cgtcttcgga ctgttcgagg tcttcatagc
     6181 tgccttcggc gtagacgata ccgtctttca gcaggatgat cctgtcggct gtgatccggg
     6241 cgcagggcat atcgtgcgtg atggtgatgg acgagatatt gtacttcctt ttcatctggc
     6301 agatgaggtc gctgatctcg cgggccgtac cggtgtcgag tcctgtggtc ggctcgtcat
     6361 agagcatgat ctccggacgc aggatcagcg tacgggcgag gccgatgcgt ttgctctgtc
     6421 ctccggatag tttggacggt aaaagatcga tcgaatccag cagccccacg ttgtcgagca
     6481 tttcattgat cagcgcttct ttcttttcct tcgacattgt ttttttgtgc tggcga
//
LOCUS       DASYSV010000018         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_261233, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000018 DASYSV010000000
VERSION     DASYSV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261233"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..2176
                     /locus_tag="VGQ51_00545"
     CDS             170..2176
                     /locus_tag="VGQ51_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9035066.1"
                     /translation="MNKFYVALLLVFAADVLQAQSPGGVSTNLSVWLKADNSSTLSPT
                     TGSLNSWTYSNNGSNSYTAAAGQQPTVAANTFNFLPSIAFNGTQFMSGPAGASAPIPA
                     GQMNYSVFAVWSSTTASGGANERIWVQRPNSNAFDNSGDGAALWVYPSGGPTYGDQAE
                     IGPGFTYGVYFAPAYNTPILTYAPNTPYISEMNLLNAGTDDLELMDQTNYATGPGVTT
                     TSPAGATATRNLSNGANLLGARSTSTAPFDEPFFGNLAELIIYSGNVTPAQRSAIFSY
                     LSIKYGIPLGGDYVSSAGTAIWESALNGNWGSGATNFNYNHFVFGLGLDNGSGLSTTQ
                     SNSLSTGSGSGSGQSGLGNIVLSNPGSLTDQGFMLVGSNNQGFAETTTNLPSSSPVGA
                     KRLATQWLVQNTGSVGTVDLSFDFTGITTTGTIGTSSDFALAVDNDGDGDFTTGTPSV
                     YAPNSWNGNVANFTGVSINGSKRVVLSILSNSNNTPLPVNWVNFTGRANGPDVDLNWT
                     VSANENARVYDVQRSADGSSDFTSIGKVTNDANVKSYGFVEANAGPGTHYYRVLETDQ
                     DGKSIYSKIVSVNMSGSDFGVRLLNNPVVNGMDAQLEITAANSGNASVEIWALGGTRV
                     ATMMQNFPNGTSKIRLPMANLASGTYAVKIRVNDVTQTVQIVKL"
     gene            complement(2222..3394)
                     /locus_tag="VGQ51_00550"
     CDS             complement(2222..3394)
                     /locus_tag="VGQ51_00550"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9035067.1"
                     /translation="MSIWLSLFVACCFIVIYNYAGYAIIAWLFNRLRRPRTLPHLADH
                     QLPGISFIVAAYNEEDCIAAKITNSLELDYPREKIEFLFISDGSNDHTESIVLAHPDI
                     RSLHRPERAGKSAALNRAVGEAGKEILVFSDANTVLNRESLRRIAHHYQDPSIGGVAG
                     EKKVLPAPGSGEVGQAEGLYWKYESYLKKIDAAFYSVVGAAGELFSFRRSLFEPIPST
                     VILDDFVSSLKVCQRGYRIAYAPDAYAMELPSATLGDEAKRKVRIAAGGFQAIGMLKG
                     LFAFWKYPRLSYLYISHRVLRWAVSPFCLIGAFIANGMLVLEHAGLVFTILFLAQCCL
                     YAAAALAALVTGLQRFKPIKLAYYFVFMNTCAILGFLRHLKGNQSSVWEKSRRATA"
     gene            3582..>3932
                     /locus_tag="VGQ51_00555"
     CDS             3582..>3932
                     /locus_tag="VGQ51_00555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9035068.1"
                     /translation="MRPDDHKRRIAVLVPAYKEDGIILSTARNLLQQDYPADRYHVFI
                     IADSFQPETIGVLKQLPLTVLEVSFEKSTKTKSLNDAFKRIGEPFDIALVCDADNMLA
                     SDFLQRMNSAFGAGA"
BASE COUNT          876 a          941 c         1151 g          964 t
ORIGIN      
        1 gaatatcgat atctttatgg cctctctgaa actgctttcg tcggatcgtc tgatctccta
       61 tgtgattcct acgctaacta atccatctcc aatttcctgt tggaattaaa tgctgctgtg
      121 tgccggcctg gtcttcccta ctttgtaatc gtcatttaaa acgtataaaa tgaataaatt
      181 ttacgtagcc ctactactcg tctttgccgc ggacgttctc caggctcaga gtccgggagg
      241 ggtaagtacc aatctttccg tttggttgaa agcagacaac tcttccactt tgagtcctac
      301 caccggcagt ctgaatagct ggacctattc caataatggc agcaattctt acaccgccgc
      361 ggcgggtcag cagccgacgg tggctgcgaa tactttcaat tttctgcctt ctattgcttt
      421 taatggtaca cagttcatga gcgggccggc gggcgcaagt gctccgatac cggcgggaca
      481 aatgaattat tcggtcttcg cggtgtggtc ttcgacgact gcttccggag gagccaatga
      541 gcggatctgg gtgcaacgtc ccaactcgaa tgcattcgac aacagcggcg acggtgccgc
      601 gctgtgggtc tatccttccg gcggccctac ctatggcgac caggccgaga tcggacctgg
      661 ctttacttat ggtgtttatt tcgctcctgc ttataacacc ccgatcctca cctatgcacc
      721 caatacgccc tatatcagcg agatgaacct gctcaatgcg ggtactgacg acctggaatt
      781 gatggatcag accaattatg ccacgggccc cggcgtaacc actacttccc cggcgggcgc
      841 cactgcgacc cggaaccttt cgaacggggc caatcttctg ggggcgagga gtacgtctac
      901 agctcctttt gacgagccat ttttcgggaa tctcgcagag ttgatcatat attcgggtaa
      961 tgtcactccg gcgcagagaa gcgcgatctt ttcgtatctc tcgataaaat atggcatacc
     1021 gttgggagga gactatgtgt cttcggcggg tactgccatc tgggagtcgg ctctgaacgg
     1081 aaactggggt tccggcgcga ctaatttcaa ttacaatcat tttgttttcg gtcttggtct
     1141 ggacaatggg tcgggtcttt cgaccacgca atcgaatagt ctttccacgg gtagcggcag
     1201 cggctcgggt caatcggggc ttggcaatat cgtattgtcg aatcccggct cgctgaccga
     1261 tcagggtttc atgctggtgg gcagcaacaa ccagggtttt gccgaaacca cgaccaatct
     1321 tccctcttcg tctccggttg gcgccaagcg acttgccacg caatggctgg ttcagaacac
     1381 cgggtcggtc gggacggtcg atctaagctt tgatttcacc ggcattacca cgaccggtac
     1441 tattggaact agcagcgatt tcgcgttggc cgtagacaat gacggcgacg gtgatttcac
     1501 taccggaacg ccgagcgtct atgcacctaa ttcctggaat ggaaatgtgg ctaatttcac
     1561 cggtgtatcg atcaatggca gcaagcgggt ggtcctttct atcctttcga attcgaacaa
     1621 cactccgttg ccggtcaatt gggtcaattt caccggcagg gccaacggtc cggatgtgga
     1681 cctgaactgg acggtcagtg ccaatgagaa tgccagggtc tatgacgtac agcgttctgc
     1741 cgacggatcg tctgacttta cctccattgg taaggtaact aacgacgcga atgttaagag
     1801 ctacggcttc gttgaagcaa acgccggacc aggcacccat tattatcgtg tactggagac
     1861 agatcaggac ggcaagtcca tctatagtaa gatcgtttcg gtcaatatga gcggatcgga
     1921 tttcggggtg cggctgttga ataatcctgt ggtgaacggt atggatgccc agctggagat
     1981 aacggctgcc aattcgggca atgcttcggt ggagatctgg gcattgggcg ggacgcgtgt
     2041 agcgacgatg atgcagaact tcccgaacgg aacttcgaag atacggttgc cgatggcgaa
     2101 cctggcatcg ggtacatatg cggtgaagat cagggtgaat gatgtgacgc agaccgtgca
     2161 gatcgtgaag ctgtaatagg attcggcgaa ataattaaaa cccacctaac ggtgggtttt
     2221 tttatgcggt ggcgcgacgg gatttttccc agacggatga ctggttgcct ttgaggtggc
     2281 ggaggaatcc gaggattgcg caggtgttca tgaagacgaa atagtaagcg agctttatgg
     2341 gtttgaatcg ttgcaggccg gttaccaggg cggcgagggc ggcggcggca tagaggcagc
     2401 attgggcgag gaagaggata gtgaagacga ggccggcatg ttcgaggacg agcatgccat
     2461 tggcgatgaa ggccccgatc agacaaaacg ggctgacggc ccagcggagt acgcggtggg
     2521 agatatataa ataggagagg cgggggtatt tccagaaagc gaaaaggcct ttgagcatgc
     2581 cgattgcctg gaagccgccg gcggcgatgc ggactttcct tttcgcttcg tctcccaggg
     2641 tggccgaggg gagttccatc gcgtaagcgt ccggtgcata tgcgatacga tagccgcgtt
     2701 ggcatacctt gagcgaactg acgaaatcat cgaggatcac ggtggaaggg atgggttcga
     2761 aaagcgatcg ccggaatgag aagagctcac ctgccgcgcc gacgacggaa tagaaagccg
     2821 cgtctatttt tttgagatag gattcatatt tccagtagag gccttcggcc tggcctactt
     2881 cgccggaacc gggagcggga agaacttttt tctcgccggc gaccccgccg atggatgggt
     2941 cctggtagtg gtgggcgatc ctgcggagcg actcgcggtt cagcaccgta ttggcgtcgc
     3001 tgaaaacgag gatctctttt ccggcctctc ctaccgcgcg gttgagggct gcggatttgc
     3061 ctgcgcgttc gggccggtgg agtgagcgga tatccgggtg ggcgaggacg atcgattcgg
     3121 tatggtcgtt gcttccatcg ctgatgaaaa gaaattcgat cttttcccgc gggtaatcca
     3181 gttcgaggct attggtaatt tttgccgcga tgcaatcttc ttcgttgtag gctgccacga
     3241 tgaaagagat acccggtagc tgatggtcgg cgaggtgggg gagggtgcgg gggcgccgga
     3301 gcctgttgaa cagccaggcg atgattgcat agccggcata attatagata acgatgaagc
     3361 agcaggcgac gaacaaagag agccaaatcg acataggagt aaattatgga agtaaaaata
     3421 atgtatcgta aacaagagaa tcattatttt gcaatatgtt atttaataaa ataatatacc
     3481 tctgtttttg ggtttgtttt gtgtattgtg cggggtccgt agcctatctt tttctcctct
     3541 cattgtccgg ccgtcttttt tcgcgcaaga aaatggacag gttgaggccc gatgatcaca
     3601 agcggcggat agcggtgctg gtgcctgctt acaaggagga tggcatcatt ctttctacgg
     3661 cccgcaacct gttgcagcag gattatccag ccgatcgtta tcatgtattt atcattgccg
     3721 atagttttca gccggagacc atcggggttt tgaaacagct gcccctgacg gtactggagg
     3781 tgtcttttga aaagagcacc aagacgaaat cattgaatga tgcattcaag aggatcgggg
     3841 agccattcga catcgcgctt gtctgtgatg cggacaatat gcttgcgtcg gatttcctgc
     3901 agcggatgaa ttccgccttc ggggccgggg cg
//
LOCUS       DASYSV010000019         3535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2181691, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000019 DASYSV010000000
VERSION     DASYSV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181691"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VGQ51_00560"
     CDS             complement(<1..198)
                     /locus_tag="VGQ51_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV9035069.1"
                     /translation="MNLFDVYPINEITIVKAKGSYVWDQDGQQYLDLYGGHAVISIGH
                     THPHYVQRITDQLGKVGFYSNS"
     gene            complement(218..1186)
                     /gene="argC"
                     /locus_tag="VGQ51_00565"
     CDS             complement(218..1186)
                     /gene="argC"
                     /locus_tag="VGQ51_00565"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEV9035070.1"
                     /translation="MRQVRAGIIGGAGYTGGEMIRLLINHPAADIRFIHSKSNAGKLV
                     SDIHEDLAGDTVLTFDSEWHSEVDVVFLCMGHGESKKFLDEHGPSFDGVIIIDLSQDF
                     RLSGTPWVYGLPELNREHIRSAQAVANPGCFATAIELGLLPLAKAGLLHDVYTTGITG
                     STGAGQSLSTTGHFSWRANNIQAYKTLTHQHLKEIGHSLRHLQPNGVPPSVHFTPWRG
                     DFTRGIFISSQLSCEKDADEIRALYEDYYRSHPFTIVSRNPISLKQVVNTNKCLIQLE
                     QVGRQLVVHSALDNLLKGAAGQAVQNMNLLFGLEETAGLRLKANAF"
     gene            complement(1261..2457)
                     /locus_tag="VGQ51_00570"
     CDS             complement(1261..2457)
                     /locus_tag="VGQ51_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEV9035071.1"
                     /translation="MSDKVVLAYSGGLDTTYCAVWLNKVKHLEIHALTVNTGGFGKEE
                     IDQIAARAKSLGVASFECVDVTREYYDSCIKYLIFGNVLKNATYPLSVSAERMVQAIA
                     VANHVKKVGAQYVAHGSTGAGNDQVRFDMVFQSMLPGVEILTPIRDLRLSREEEIDFL
                     KNNGVEINTEKARYSINKGLWGTSVGGKETLTSKDYLPETAWPTQVTSTDTRNVTLEF
                     QHGELRSVDGKTFPHPVAAIQYLQSIAQPYGIGRDIHVGDTIIGIKGRVGFEAAAPII
                     IVKAHHLLEKHTLTKWQLYWKDQLSSWYGNWLHEGQMLDPTMRNIEKFLQDTQTTVSG
                     KVFVTLQPYRYTVTGIESPHDLMSSKFGSYGEMNKGWSGDDVKGFAKIFGNQTAIFHK
                     VNESDS"
     gene            complement(2473..3156)
                     /locus_tag="VGQ51_00575"
     CDS             complement(2473..3156)
                     /locus_tag="VGQ51_00575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9035072.1"
                     /translation="MEQQQFKIVIANATHQDFARIICDEMESSAKARGTGIAKRSPEY
                     IQQKMREGKAVIAFTDDGVWAGFCYIETWSHGEYVANSGLIVSPSFRKSGLARMIKRK
                     IFDLSRKTYPDAKIFGLTTGLAVMKINSELGYEPVTYSELTQDNDFWAGCKSCVNYEI
                     LMSKERKNCMCTAMLYDPKDHYEPEETTQDFRKHSKLYERFMRIKQWKLLAPFLKHHA
                     SSLIQQFFH"
BASE COUNT          737 a          825 c         1067 g          906 t
ORIGIN      
        1 ggaattgctg taaaagccta cttttccaag ctgatcggtg atgcgttgca cataatgcgg
       61 gtgtgtatga ccgatagaaa tgacggcatg gccgccatag aggtcgaggt attgctggcc
      121 gtcctggtcc cagacgtaag aacctttggc tttgacgatg gtgatctcat ttatcgggta
      181 aacatcaaat aaattcataa tgatctagtt tatattttta gaaggcatta gcttttagcc
      241 ggagaccggc ggtttcttcg agaccgaaaa ggagattcat attctggacg gcctgacccg
      301 cggctccctt cagcagattg tcaagcgcgg aatgcacgac cagctgccgg cctacctgtt
      361 ccagctggat caggcattta ttggtattga cgacctgttt cagcgaaatg gggttacggc
      421 ttacgatggt aaaggggtgg gaacggtaat agtcttcgta tagtgcgcgg atctcgtccg
      481 catctttttc gcaggacagc tggctgctga tgaagatgcc gcgggtgaag tcgccgcgcc
      541 agggtgtgaa atgtacggat ggggggacac catttggttg caggtgccgc aacgagtggc
      601 ctatttcttt cagatgctga tgcgtcaggg tcttgtaggc ctggatattg ttggcgcgcc
      661 aggagaaatg gccggtcgtg gaaagggatt ggccggcacc ggtgctgccg gtgatgccgg
      721 tagtatagac gtcgtggagc aggccggcct tagccagcgg gagcagaccg agttcgatgg
      781 cggtggcgaa acagccgggg ttggcgacgg cctgggcgga gcggatgtgt tcgcggttga
      841 gttccggcag gccatagacc cagggcgttc cgctaagacg gaagtcctgg gagaggtcga
      901 tgatgatcac gccgtcgaac gaggggccat gttcgtccag gaattttttc gactcgccgt
      961 gtcccataca aaggaagact acgtcgactt cgctgtgcca ttccgaatcg aaggtcaaaa
     1021 cggtgtctcc ggcgaggtct tcatggatgt cggagaccag tttgccggcg ttgcttttgc
     1081 tatggatgaa gcggatgtcc gcggcggggt gattgatcaa tagccggatc atttctccgc
     1141 cggtgtagcc ggcgcctcct attatgcctg cgcgtacttg tctcatatcg atgctattta
     1201 tgattggccg atggtccggt tggcgccggg gggccgatcc tattctgtcg tttgcggtgg
     1261 tcatgaatcg gattcgttga ccttatgaaa gatggccgtc tggttaccga agatctttgc
     1321 gaaacccttg acatcgtcgc cgctccatcc cttattcatt tcaccatagc tgccgaattt
     1381 gctgctcatc aggtcgtggg ggctttcgat accggtgacg gtgtagcggt aaggttgcag
     1441 ggtgacaaag accttgccgg aaacggtggt ctgcgtgtcc tgcaggaatt tttcgatatt
     1501 gcgcatcgtc gggtcgagca tctgtccttc atggagccag ttgccgtacc agctgctgag
     1561 ctggtccttc cagtagagct gccatttggt cagcgtatgt ttttccagaa ggtgatgcgc
     1621 cttaacgatg atgatcggcg cggcggcttc gaacccgacg cggcccttga tgccgatgat
     1681 ggtgtcgccg acatggatat cgcggccgat gccatagggc tgggcgatgg actgaagata
     1741 ttgaatggcc gcgacgggat gagggaaggt cttcccgtct acggacctga gctcgccgtg
     1801 ctgaaattcc agcgtgacat tgcgggtatc cgtgctcgtt acctgcgtag gccaagcggt
     1861 ttcgggaaga tagtctttgg aggtgagtgt ttctttaccg ccgacggagg tgccccagag
     1921 acctttattg atactgtatc tggctttttc ggtgttgatc tctacgccgt tgtttttgag
     1981 aaagtcgatc tcctcttcgc ggctgagcct gaggtcgcgg atgggagtga ggatctccac
     2041 gccggggagc atgctttgga agaccatatc gaaacggacc tggtcattcc cggcgcccgt
     2101 acttccgtgc gcgacatatt gtgcgccgac cttttttacg tggttggcca cggcgatcgc
     2161 ctgtaccatt ctttcggcgc tgacgctcag cggataggta gcgttcttga ggacattgcc
     2221 gaagatgagg tatttgatac aactgtcata atattccctc gtaacgtcga cgcattcgaa
     2281 agaggcgacg ccgagacttt tagcccgggc tgctatctgg tcgatctctt ccttgccgaa
     2341 ccctcctgta ttgacggtga gggcatggat ctccagatgt tttaccttat tgagccagac
     2401 ggcgcagtag gtggtgtcca atccgccgct gtaggctaag acgactttat cgctcatgag
     2461 aggggggtga gtttagtgaa aaaattgttg aatgagtgag gacgcgtggt gtttcaggaa
     2521 gggggccagc agcttccatt gtttgatacg catgaagcgt tcgtacagct tggaatgttt
     2581 tcggaagtcc tgtgtcgtct cctcgggttc atagtggtct ttgggatcgt agagcatggc
     2641 ggtacacata caattcttcc tttccttgct catgaggatc tcgtagttga cgcaactctt
     2701 gcatccggcc cagaaatcat tgtcctgggt cagctcggaa taggtgacgg gttcgtaacc
     2761 cagctcggag ttgatcttca tcacggccag accggtggta agaccgaata ttttggcatc
     2821 gggataggtc ttacgggaga gatcgaagat cttccgcttg atcatcctcg ccagaccgga
     2881 cttccggaag gacggggaaa cgatgaggcc agagttggcg acatattcgc catggctcca
     2941 ggtttcgata tagcagaaac ccgcccagac gccgtcatcg gtgaaggcga tgaccgcctt
     3001 accctcgcgc attttttgct ggatatattc cgggcttcgt ttggctatgc ccgtacccct
     3061 cgctttcgcg gaggactcca tttcatcaca aatgatccgg gcgaaatcct gatgggtcgc
     3121 gttggcaata actatcttga attgttgttg ttccattgtg tttaatgatg gtcgttccca
     3181 tcaataagaa aaaaagataa tcggctggac tggaaaatag cgtcgttaag acggggggcg
     3241 cttctatagt aaagttgtat caacgtcgta ccggaacggg cgtggggcgc ccgtaattat
     3301 aattagcccc gggtgcagac ggcgtataat tagcctcggg gcggacggaa aaaactgggc
     3361 acagtggcac agcaaaaaac ccacgcttat tgaaggcgtg caatatcacc tggaaacgtg
     3421 atagtgagtt ttttttcatt ggttcaaaaa attacaacgc aaagaaagac aattttttgg
     3481 gaatggggcg ggtttggcgg caaaatttta tgcaaaaatt ttgcgggggg cgccg
//
LOCUS       DASYSV010000020         4262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1669717, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000020 DASYSV010000000
VERSION     DASYSV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669717"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VGQ51_00580"
     CDS             complement(<1..1011)
                     /locus_tag="VGQ51_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV9035073.1"
                     /translation="MGDLQIKKQAKKYDAVIVGSGAGGGMAAYVLAQAGLKVCLLEAG
                     PMFDPKKDSNQLKNPWDSPRRGAGTKFRPFGDFDGCYWGWEVEGEPYTQTGNKGTTAW
                     EWWRARMLGGRTNHWGRISLRFGPKDFKRKSIDGLGDDWPIGYEDIKPYYDKIDRLLG
                     VFGTNEGLENDPDGIFLPPPKPRLHELMLKKAGASVGVPIIPSRLSILTKVTKDMDPS
                     RGQCFFCAQCGRSCKVYGDFSSSSCLVIPAIKTGNVDLVTNAMAREVLTDREGLATGV
                     SYVHKDDMQEYQVFGRTVILAASACESSRLLLNSKSARHPNGLANGSDVVGRYLHDST
                     GAD"
     gene            complement(1101..2762)
                     /locus_tag="VGQ51_00585"
     CDS             complement(1101..2762)
                     /locus_tag="VGQ51_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEV9035074.1"
                     /translation="MISTAFRAVLFGTIAGAILSCNQGGDKTGDEGIQAFNKDTLASD
                     IKTLASDDFQGRRPFTPGETKTIDYLVNAYSRLGLEPGNGNGYTQDVPLVEISPSAPP
                     VLEVHSPKGAVHLQNMTDFVCWTERPDTVDAVDKSDIVFAGFGVVAPEYNWNDYAGLD
                     VKGKTVIVMVNDPGFYDSTLFKGHTMTYYGRWTYKYEEAARQGAKACLIIHNTAAASY
                     PFSVVQSSNGGVKLHLDTRSAPAYQLALQGWITEDAAKRIFAAAGKDSSLLKAAQHRG
                     FKPTPLDLTLSGSVRVKEVYNMSHNVIGKITGSKYPDEYVIFSAHWDHLGIGKPDANG
                     DSIYNGAADNASGTASLLQIARAFKSLPDKPERTVIFLSVTAEEQGLLGSAWYGQHPV
                     YPLAKTVADLNIDVINTYGPTKDITYSGNGQSTLEDDLAQEVKAKGRYIAPEDHPEAG
                     HYFRSDHFSFARAGVPSLTADGGVDDVNKGVAYGKQKHDEYTANRYHQPSDQYDSTWN
                     LDGGMQDIELVYNVGKRLSFSHEWPEWKSGSEFKAIRDKSAAERK"
     gene            complement(2766..3818)
                     /locus_tag="VGQ51_00590"
     CDS             complement(2766..3818)
                     /locus_tag="VGQ51_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV9035075.1"
                     /translation="MTTSMIRNLLLPALIATAFCLPAHGQSDSMGMPCMEKAERLLDE
                     ALTFMEKNYYRKDEISWPEFRDRARQELRTAGNCEDAYASISWCFRQLNEPHSFVMAP
                     DKAARYNGEAASSTALSALVGQIRGEWLQDSIGYLTVPWVSTDDSLLCERIADSMQSV
                     IARLDSRGIARWIIDLRQNSGGNCWPMLTGIGPLLGDGVYGYFVSGDERVPIAYREGS
                     ALQGRHVRCRVSNQGYRTQKDRKYIVILTGPGTVSAGETVALAFKGRSLTYFIGQPTA
                     GLTTANATYPLSDRSMLVLTICQEADCTGRVCQGRIRPDMLVPPAAGNTSPDPGNDPV
                     KEAAVSWLTTTNFSEK"
     gene            3893..>4262
                     /locus_tag="VGQ51_00595"
     CDS             3893..>4262
                     /locus_tag="VGQ51_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV9035076.1"
                     /translation="MGYTRRAFLQNSALAVAAAGLLPGESFAFYKKEILGLQLYSVRD
                     DMNKDPAGTLKQVAAMGYRYVEHAGYGNGKFYGYEPKAFKDLLDGLGLKMISGHTAMG
                     PTAWDASKNDFSDAWKKTVGD"
BASE COUNT          841 a         1162 c         1266 g          993 t
ORIGIN      
        1 atccgcgccg gtggaatcgt ggagatatct tccgacaacg tcgctgccgt tggccagacc
       61 gttgggatgg cgcgcggatt tggaattgag aagaagccgg gagctttcac aggcactggc
      121 cgccaggatc acagtccttc cgaagacctg gtattcctgc atatcgtctt tgtgtacata
      181 ggatacgccc gtagccaatc cctccctgtc ggtgagcact tcgcgcgcca tggcattggt
      241 caccagatcc acattaccgg tctttattgc gggaattacg agacaggacg acgacgagaa
      301 atcgccgtat accttgcagc tgcgtccgca ttgcgcgcag aagaagcatt gtcctctgga
      361 aggatccatg tctttggtca ctttggtcag gatcgaaaga cgcgaaggga tgatgggcac
      421 tcccacgctg gcgccggctt ttttgagcat tagttcgtgc agccgcggtt tgggcggcgg
      481 aagaaagatg ccgtcgggat cgttctccag tccttcgtta gtgccgaaga ctccaagcaa
      541 gcggtctatt ttgtcgtagt agggtttgat atcctcataa ccgatgggcc agtcatcgcc
      601 aagtccgtcg atgcttttgc gtttgaagtc tttcggaccg aaacgaagcg agatacggcc
      661 ccagtgattg gtccggccgc caagcatacg ggcgcgccac cactcccagg cggtggtgcc
      721 cttgttcccg gtttgcgtgt agggctctcc ctcgacttcc catccccagt agcagccgtc
      781 gaaatcgccg aacggccgga acttcgtacc cgcgccgcgc cggggtgagt cccagggatt
      841 tttcaattga ttgctgtctt ttttcgggtc gaacatcggg cctgcttcaa gcagacatac
      901 ttttaacccc gcctgggcga gcacatatgc cgccatgccg ccgccagcgc cggagcccac
      961 tatcactgcg tcgtatttct tcgcttgttt ttttatctgg agatctccca tttctttatg
     1021 cgattgaggt gacgaattta aaggtttagc taaacagcaa acaaatttta tttgcggttg
     1081 cggcggttcg ggtgcgggtt ttatttcctt tcggcggccg atttgtcgcg gatggctttg
     1141 aattccgaac ccgatttcca ttccggccat tcgtggctga acgacaatct cttcccaacg
     1201 ttatagacca gttcgatatc ctgcataccg ccgtcgaggt tccaggtgga gtcgtattga
     1261 tccgaaggct gatgatagcg attggcggtg tattcgtcat gtttctgttt gccgtaggcc
     1321 acacctttgt tcacgtcatc gacgccgccg tcggccgtca gcgaaggaac gcccgcgcgg
     1381 gcaaagctga agtggtccga acggaaatag tgaccggctt cgggatgatc ttccggcgcg
     1441 atataccggc ctttggcctt cacctcctga gccaggtcat cctcaagggt ggattgaccg
     1501 ttgccgctgt acgtgatgtc tttggtgggg ccgtaggtat tgatcacgtc gatgttgaga
     1561 tcggccactg tttttgcgag gggatatacc ggatgctggc cgtaccaggc agaaccgagc
     1621 agcccttgtt cttccgccgt caccgatagg aaaatgaccg ttcgctccgg tttgtcgggc
     1681 aggcttttga aggcccgggc gatctgcagc agcgacgctg tgccggatgc gttgtccgct
     1741 gcgccattat agatggagtc gccgtttgcg tcgggcttac cgatccccag gtggtcccag
     1801 tgggcggaga agatgacgta ttcatccggg tatttgctgc cggtgatctt cccgatgaca
     1861 ttgtgcgaca tattgtacac ttcttttacc cgcaccgaac cggacagtgt aagatccagc
     1921 ggggtcggtt tgaaaccccg gtgttgagca gcctttaaca ggctgctgtc ttttcccgcg
     1981 gccgcgaaga tccgttttgc ggcatcttcc gtgatccagc cctgcagcgc cagctgatat
     2041 gcgggagccg agcgcgtgtc gagatgcagc ttgacgccgc cattgctgct ttgtacgacg
     2101 ctgaacggat aggacgccgc cgccgtgttg tggatgatca gacaggcctt cgcgccttgc
     2161 cgggccgctt cttcatattt ataggtccat cggccgtagt aggtcatggt atgtcctttg
     2221 aagagggtcg aatcgtagaa gcccggatcg ttcaccatga cgatgacggt tttccctttc
     2281 acgtccagcc ccgcatagtc attccagtta tattccgggg cgacgacgcc gaacccggcg
     2341 aatacgatgt cgcttttgtc cacggcatcg acggtgtcgg gcctttccgt ccagcaaacg
     2401 aagtcggtca tgttctgcag gtgtacggcg cctttggggg aatgtacttc cagcacggga
     2461 ggcgcggacg ggctgatctc cacgaggggc acgtcctgcg tgtagccgtt gccgttgccc
     2521 ggttcgagac ccaggcggga gtaggcgttc accaggtaat cgatcgtttt ggtctcgccg
     2581 ggcgtaaaag gacggcgtcc ctggaagtcg tcggaagcga gggttttgat gtcggatgcc
     2641 agcgtatcct tgttgaaggc ctgaattcct tcatcgccgg tcttgtcgcc gccctggttg
     2701 caggaaagta ttgcgcccgc gatcgtcccg aaaaggacgg cgcgaaatgc ggtagagatc
     2761 atagactatt tttcgctgaa gttagtggtt gtaagccaac taacggcggc ttctttcacc
     2821 ggatcgttcc ctgggtcggg agaagtatta ccggcggcgg ggggaacgag catatcgggg
     2881 cggatgcgtc cctgacagac gcggcccgta cagtcggctt cctggcagat ggtcaggacg
     2941 agcatagacc gatccgacag cggataggtg gcattggcgg tagtaagtcc tgcggttggc
     3001 tgaccgatga aataggtaag cgatcgtcct ttgaatgcca gggcgacggt ctctccggcg
     3061 ctgacggtgc cgggacccgt caatatgacg atatatttac ggtctttttg cgtccggtag
     3121 ccctgattgc tcaccctgca gcggacgtga cggccctgta aggccgaacc ttcacgatag
     3181 gcgatgggta cccgttcgtc tccggagacg aaatagccgt agaccccatc accgagcaag
     3241 gggccgatgc cggtgagcat cggccagcag ttgccacccg aattttggcg gaggtcgatg
     3301 atccatcggg cgatgcccct ggaatccaga cgagcgatga cggactgcat gctgtctgca
     3361 atgcgttcgc agaggagcga atcgtcggtt gacacccatg gaacggtcag atagccgatg
     3421 ctatcctgca accattcacc ccggatctga cccaccagcg cagatagcgc cgtagaggac
     3481 gctgcttcgc cgttatagcg ggctgccttg tcgggagcca tgacaaagct atggggttcg
     3541 ttcagctgcc ggaaacacca gctgatggag gcgtaggcgt cttcgcagtt gccggcggta
     3601 cgcagctcct gacgagccct gtccctgaac tccggccagg agatctcgtc cttccggtag
     3661 tagttctttt ccatgaaggt aagggcttcg tcgagcagcc gttcggcttt ctccatacag
     3721 ggcataccca tggaatctga ttgtccgtgg gccggcaggc agaaggccgt ggcgatcaac
     3781 gccggcaaaa gcaggtttcg gatcatggat gtcgtcattg gatgagggtt aagatagcaa
     3841 aaaacaggaa ttccataaaa ttcattacat tcatccatca attcgaatgc acatgggcta
     3901 cacccgcaga gcttttcttc aaaacagcgc tttggccgtc gccgccgccg gcctgctgcc
     3961 cggcgaatcc ttcgccttct ataaaaaaga gatcctcgga ctccagttgt acagcgtacg
     4021 ggacgacatg aataaagatc cggccggcac gttgaagcag gtggccgcaa tgggttatcg
     4081 ctacgtcgag catgccggct atggaaatgg caagttctac ggatatgagc ccaaggcatt
     4141 caaggacctg ctcgatggtc tcggtctgaa aatgatcagc ggtcataccg cgatgggacc
     4201 cactgcctgg gatgcttcca aaaacgattt cagcgatgcc tggaaaaaga cggtcgggga
     4261 cg
//
LOCUS       DASYSV010000021         6448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1670368, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000021 DASYSV010000000
VERSION     DASYSV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670368"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2146
                     /locus_tag="VGQ51_00600"
     CDS             <1..2146
                     /locus_tag="VGQ51_00600"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HEV9035077.1"
                     /translation="MKTRHLPDRILGQKVEQQDKKDMGVDSAGKGIIFLSESLTKVFF
                     RRPNKIKMEVLSGRQSGSNGYGFTLPTFINFYDNNVAVLGNLSPRGFVSPIADGALGY
                     YRYKFLGSWFEDGKEINEIQVIPRRKFEPLFSGKIDIVEGDWRIHSLDLLLVKESQLQ
                     LLDTLEIKQLHAPVTGGGGGSAGSGSGGDVWQVKNQVVYFTFNILGVDAVGNFLNIYN
                     NYDIQPKFKRKFFNNVLIRYDTAGNKRTRTYWDSIRPLPLEPDEKINYTIRDSIYRYN
                     PDSMGTRKNRDSLLRKQGHIKAMDFLTGFNRSNFRAPAPLRYSLSPLFSGFHYNTVEG
                     INWTVSGSVSKRLGGLGELELAPHIRYGFHNTRLNPWGILTLRRRKFVREEESISSSR
                     QTWTLAGGKRVEQFDASEPISETVNDAYTLLGGDNFLKEYEKYFVQLGSDTRWDNGLR
                     MSVRAGWEDRIPVSNTTLYTWASTAKQSFTPNYPVERLDTSFPRHRAVVTSVELEYQP
                     GRHFIEFPDRKVSLGSKYPTLSLRYTKGWDGVMGSSVNFDKWQFSVRDDINLKLLGQF
                     KYRLVAGGFLNTGAVYLQDYAHFNGNQTIVAGDYLHSLQMAPYYAYSNTASLYGAGYT
                     EHHFNGLLTNKIPLFRRLNWNLVGGFNVFYANGASNFEEMSWGLENIFKIFRVDILTS
                     WREGSYFQTGVRIGMGGLLGDGMRRAPASGGR"
     gene            2207..3259
                     /locus_tag="VGQ51_00605"
     CDS             2207..3259
                     /locus_tag="VGQ51_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV9035078.1"
                     /translation="MPVLHNRVSQRELKERLSKEKEPRTTISFYRYFPIPDPRVFRDE
                     LYAGLSGLSVFGRIYVAAEGINAQISVPASKCGDLQRWLDSVQGLEGLRLNIAVDDDG
                     KSFWVLKIKVREKIVADGIGDPGFDMRRKGQYVDAAAFNRLTDDPDTTVVDMRNHYEF
                     EVGHFDGAIEVPSDTFREQLPMSAEMLKDKKDHPVILYCTGGIRCEKASAYLLYKGFT
                     NVFHLEGGIIHYANTVKEQGLENKFRGKNFVFDDRMGERISGEIIARCHQCGEPADTH
                     TNCRNEGCHLLFIQCAACAEKYEGCCSPACQEMNHLPDEQRKVLRKGTNKGLHVFNKA
                     RVRSARSTPGGRKPGV"
     gene            3536..4939
                     /locus_tag="VGQ51_00610"
     CDS             3536..4939
                     /locus_tag="VGQ51_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV9035079.1"
                     /translation="MNKAAQNFFHLREYIWNIGYRPSLGSYEKRKLGLFNFMNFMGIL
                     AGIVIPVIGLFSRGHFPPSVWVAACSPAFISLTVLVLVRHEKYEVARILYFTLYPLMT
                     LLTYFEHIDAGIEFFFVLYGVLSVFFLKNIYNIIGAFTLSMTCYFLIVAVWREYDYRL
                     EDHNFFLYLFNHVLALFLIFYGLLMIKNENSRYQNQTRDKNWQLRRSNLKILRQKADI
                     SGKAVLLEQQTQQLTELNTLKNKLFSIIAHDLKSPLYALRTLFRNVQLYDLPGDEIKL
                     LIPEVVNELTYTTGLMENLLQWAKSQMQAESVKPQLLDIAGITGEVLQLLRLQADAKK
                     IYIDSKIQRPVYVYADKDMINLVLRNLLSNAIKFTPEEGSIFIDAREMRSHIEVVVQD
                     TGTGISEEGLKRLREANYYSTRGTIGEAGTGLGLMLVKEFLSRNGGAMRIESEPGKGS
                     TFAFTLPKGGGEGHALS"
     gene            complement(4983..5912)
                     /locus_tag="VGQ51_00615"
     CDS             complement(4983..5912)
                     /locus_tag="VGQ51_00615"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV9035080.1"
                     /translation="MSKQLQFPHQTSFYRGKVRDVYTISDKWLVMIASDRISAFDVIL
                     PRPIPYKGQVLNQIAAYMLRATADICPNWLIDTPAPNVAVGRRCVPFRVEMVVRGNLT
                     GHAWRTYRSGQRELCGVPLPGDMKENDYFPSPIITPSTKAEVGHDEDISREDIIRRGL
                     VSADHYAVLEKYTLALFARGRELAAKRGLILVDTKYEFGLLDDAVCLMDEIHTPDSSR
                     YFYANGFEERQQKGERQQQLSKEFVREWLIANDFMGKEGQTVPAMNDEWVDTISKRYI
                     ELYERVIGEPFQPKSLSDEEIYQRALYSVGNLS"
     gene            6022..>6448
                     /locus_tag="VGQ51_00620"
     CDS             6022..>6448
                     /locus_tag="VGQ51_00620"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9035081.1"
                     /translation="MRKKLSPIPVLAILLLMLVSSPSFSQNSITVSGNVRQAVSGDRV
                     PAVSVTIKGTSIGTYTDEHGNFRLTTNQQPPFVLVFSSIGFETREVQVAGASNAITVD
                     LAAASTLGREVVVSATRGAIRSLESPVSIERMGATTAHEV"
BASE COUNT         1509 a         1621 c         1867 g         1451 t
ORIGIN      
        1 gatgaagacc cgccatcttc cggacaggat actgggacag aaggtggaac agcaggacaa
       61 aaaagatatg ggcgtcgatt ctgccggcaa agggatcatc tttttgtccg aatcactgac
      121 caaagtgttc ttccggcgtc ccaacaaaat aaagatggag gtcttgtctg gcagacaaag
      181 cggcagcaac gggtatggct ttacccttcc taccttcatc aacttctatg ataataatgt
      241 cgccgtgctg ggcaatctga gcccgagggg tttcgtatcg cctatcgccg acggcgcgct
      301 gggatattac cggtataaat tcctgggcag ctggttcgaa gacgggaagg agatcaacga
      361 gatacaggtg atacctcggc gcaaattcga gccgctgttc agcgggaaga tcgatatcgt
      421 ggagggagat tggcgtatcc atagcctgga cctgctgctg gtaaaggagt cgcagcttca
      481 gctgctggat acgctcgaga taaaacagct gcatgctccc gtgacgggag gcgggggcgg
      541 gtcggcgggg agcggctcgg gcggcgatgt ctggcaggta aagaaccagg ttgtttattt
      601 caccttcaat atccttggtg tcgacgcggt gggcaacttc ctcaacatct ataacaacta
      661 tgacatccaa ccgaaattca aacggaagtt cttcaacaat gtcctgataa gatatgacac
      721 cgcggggaac aagcggaccc ggacctattg ggacagcatc cggcctctcc cgctggagcc
      781 ggacgaaaag atcaattaca cgatcaggga cagtatttac cggtacaatc ccgattccat
      841 gggaaccagg aagaaccggg attcgttgtt gcgaaaacag ggacatatca aggcgatgga
      901 ctttctgacc gggttcaacc ggagtaattt ccgggcaccg gcaccgctgc gttattcgct
      961 gagtcccctg ttctcgggtt ttcattacaa tacggtagaa gggatcaact ggacggtgag
     1021 cggatcggtg agtaaaaggc tgggcgggtt gggtgagctg gaactggcgc cgcatatccg
     1081 gtatggattt cacaataccc ggttgaaccc ctgggggata ttgacgttgc gaaggcggaa
     1141 attcgtccgg gaagaagaga gtatcagctc ttcgcgacag acctggaccc tggcgggcgg
     1201 aaaacgggtg gagcaattcg atgccagtga accgatctcc gagacggtca acgacgctta
     1261 tacattgctg ggaggagata actttctgaa ggaatatgaa aaatatttcg tacagctggg
     1321 gtcggacacc cgatgggaca acggactgcg gatgagtgtg cgtgcaggat gggaggacag
     1381 gatacccgtg tccaatacta ctctttatac ctgggcctct accgccaagc aatcattcac
     1441 tcccaactac ccggtcgaaa gattggatac gtcctttccc cggcatcgtg cggtggtcac
     1501 gagcgtggag ctggaatatc agccgggccg gcatttcatc gaattccccg accggaaggt
     1561 gtcactcggg tcgaaatatc ctacgctgag tctccggtat acgaagggat gggacggggt
     1621 gatgggcagc agcgtcaatt tcgataaatg gcaattctcc gtcagggacg acatcaatct
     1681 gaaattgctg ggtcagttca agtaccggct ggtcgcgggg ggcttcctca ataccggcgc
     1741 cgtatacctg caggactatg cgcatttcaa tggcaaccag accatcgtcg ccggcgacta
     1801 tctccatagt ctgcagatgg cgccttatta tgcgtacagc aatacggcga gtctctatgg
     1861 ggcaggctat accgagcacc atttcaacgg actgctgacc aacaaaatac ccttgttccg
     1921 gcggttgaac tggaacctgg tgggcgggtt caacgtcttt tatgccaatg gcgccagcaa
     1981 cttcgaagag atgagctggg ggttggagaa tatcttcaag atattccggg tcgatatcct
     2041 gacgtcctgg cgcgagggca gctattttca aacgggtgtg cgcataggga tgggaggact
     2101 gttgggggat gggatgcgcc gggcgccggc ttcggggggc aggtgacggt gcgggcgggc
     2161 tgtgcgccgg gcgcccggag gtcggtgaat ttgcgtaaat ttgcacatgc ctgttttgca
     2221 taaccgtgtc tcccagaggg agctgaaaga gcgcttatca aaggaaaagg aacctcgcac
     2281 gacgatatct ttctatcgtt attttcccat acccgatccc cgggtcttcc gggatgaatt
     2341 atatgccggg ttatccggac tatccgtttt cggtcggatt tatgtggccg ccgaaggcat
     2401 caacgcccag atcagcgtac cggcgtcgaa gtgcggggac cttcaacggt ggctcgattc
     2461 ggtgcaagga ctggaagggt tgcggctcaa catagccgtc gatgacgacg gcaagtcttt
     2521 ttgggtgctg aagatcaagg tgcgggagaa gatcgtcgcc gacggtatcg gagacccggg
     2581 tttcgatatg cgccggaagg ggcaatatgt cgacgcggca gccttcaacc ggctgaccga
     2641 cgaccccgat acaacggtag tggatatgcg caatcattat gagttcgaag taggacattt
     2701 cgacggagcc atcgaagtgc cgtccgatac tttccgcgag cagctgccta tgtccgccga
     2761 gatgctgaaa gacaaaaagg atcatcccgt catcctgtac tgcacggggg gcatccgttg
     2821 cgagaaggcc agcgcttacc tgctgtacaa gggttttacc aatgtattcc atctcgaagg
     2881 gggcatcatc cactacgcca atacggtaaa ggagcaaggg ctggaaaaca agttccgggg
     2941 caagaatttc gtgttcgacg accggatggg cgagcgtatc agcggggaga tcatcgcccg
     3001 gtgtcaccaa tgcggtgagc cggcggatac gcataccaat tgccgtaatg aagggtgtca
     3061 tttgctgttc atccaatgcg cggcctgcgc ggaaaaatat gaaggttgct gcagcccggc
     3121 ctgccaggag atgaaccatc tcccggacga gcagcggaag gtgctccgga aagggacgaa
     3181 taagggattg catgtattta ataaggcgag ggtgcggtcg gccaggagca ccccgggggg
     3241 caggaaacca ggagtatagg ggtatccggg gggcggaaag tcgaagtcgg ggtctaaacg
     3301 atccccggga cggcagcggc acccgggcgt ccgcggttgt ccctggcttt ccgaacagtt
     3361 tagctaaaaa catcatgtgt agtggtttta tttggaacaa aaattctatt attttcgaat
     3421 ccaaataaaa gtgtatttcg tacgtttatt atctgtcttg tagctataac gattttgccg
     3481 ggcatcgcat cgtacactgt ttgcaacctt caaaactaaa cctaaggctc cgaccatgaa
     3541 caaggcagcg caaaactttt ttcatctccg ggagtacatc tggaacatcg gttacagacc
     3601 ctccctggga agctacgaaa aaaggaaact gggccttttc aatttcatga atttcatggg
     3661 catcctggca ggcatcgtga tccccgtcat cggtctgttc agcaggggtc atttccctcc
     3721 ttccgtatgg gttgcggcct gttctcccgc ttttataagt ctcacggtac tggtgctcgt
     3781 ccggcacgaa aaatatgagg tggcacgcat cctctatttt accttatatc ccctgatgac
     3841 gctgctgacc tatttcgagc acatcgacgc cgggatcgaa ttctttttcg tcctctatgg
     3901 cgtgctgtcc gtgttcttcc tgaagaatat ctacaacatc atcggcgcct tcacgctatc
     3961 gatgacctgc tatttcctga tcgtggcggt ttggagggag tacgactaca ggctggagga
     4021 tcacaatttc tttctatacc ttttcaatca cgtgctggcg ctgttcctga tcttttacgg
     4081 gctgctgatg atcaagaacg agaattcccg ttatcagaac cagacgcgcg acaagaactg
     4141 gcagttgcgc cgcagcaatc tcaagatcct gcgtcagaaa gcggatatca gcggaaaagc
     4201 ggtactgctg gagcagcaga cgcaacagct gaccgagctg aatacgctca agaacaagct
     4261 tttctccatc atagcgcacg acctcaagtc tccgctgtat gccctgcgca ctcttttccg
     4321 taacgtccag ctgtacgatc tgccggggga tgagatcaag ctattgatcc ccgaggtggt
     4381 caacgagctg acctatacga cgggtctgat ggagaacctg ctgcaatggg ccaagagcca
     4441 gatgcaggcg gaatcggtca aaccccagct gctggacatc gcggggatca cgggcgaagt
     4501 gcttcaattg ctgcgtttgc aggcggacgc caaaaagatc tatatcgaca gcaagatcca
     4561 gcgacccgtg tatgtgtatg ccgacaagga catgatcaac ctcgtcctgc gcaatctttt
     4621 atcgaatgcg atcaagttca cgccggagga aggttcgatc tttatcgatg cgcgcgagat
     4681 gcgttcgcat atcgaggtcg tcgtacagga taccggcacg ggtatcagcg aagaagggtt
     4741 gaagcggctg agggaggcga actattatag tacgcgcgga acgataggcg aggccggcac
     4801 gggactggga ctgatgctcg tgaaggaatt tttgtcacgc aacggcggcg cgatgaggat
     4861 cgagagcgag cccggtaaag gaagcacgtt cgcgttcaca ttgccaaaag gaggcggcga
     4921 gggccacgcc ctcagctaag aaggcaatga ccggtggggt cgccgggcaa ggataccggc
     4981 cctcagctaa gatttccgac ggaatacagg gcgcgttgat aaatctcctc atcgctgagg
     5041 gattttggtt ggaagggttc accgatcacc cgttcgtaca attcaatata gcgtttgctg
     5101 atcgtatcga cccactcgtc gttcatggcg gggacggtct ggccttcttt tcccataaaa
     5161 tcgttggcga tgagccattc ccggacgaac tccttgctca gctgttgctg tctttctcct
     5221 ttttgctgtc tttcttcaaa accattcgca taaaaataac gcgaggagtc gggtgtatgg
     5281 atctcgtcca tcaggcatac tgcgtcgtcc agcaatccga actcgtattt ggtgtctacc
     5341 agtataagtc ctctttttgc ggccagctcg cggcccctgg cgaacagggc cagcgtatat
     5401 ttttccagga cggcgtaatg atccgcgctg actagacccc ggcggatgat gtcttcgcgc
     5461 gagatgtcct catcgtgacc gacttcagct tttgtcgaag gggtgatgat cggggaggga
     5521 aaataatcat tttctttcat gtccccgggg agtggcaccc cgcacaattc acgttgccct
     5581 gaccgatagg tccgccaggc atgacccgtc aggttgcccc tgacgaccat ctctacgcga
     5641 aagggaacgc atctccggcc gacagccaca tttggggcag gcgtatcgat gagccagttc
     5701 gggcaaatat ctgcggtagc ccggagcata taagcagcga tctggttcag cacttgtcct
     5761 ttataaggga taggacgggg aaggatcacg tcgaaagcgg aaatccggtc ggatgcgatc
     5821 ataaccagcc atttatcgga gatcgtataa acgtcgcgga cttttccgcg gtagaagctg
     5881 gtttgatgtg ggaattgcaa ctgcttgctc atgcccgcaa aattaaattt tcaaggctag
     5941 attacaatat gtattttagt aatacacaat gccgaacctg gaaaaaccgg ggtcggaggc
     6001 ttatcaaaga aaaaacgtgc aatgagaaaa aaactatcgc ccattccggt attggccatt
     6061 ctgttgctta tgctggtttc ctctccctcc ttttcacaga attccattac cgtttccggg
     6121 aatgtccgcc aggccgtgag cggcgaccgg gttcccgcgg tcagcgtgac catcaagggt
     6181 acctctatcg gaacgtatac ggacgagcac gggaatttca ggctgaccac gaatcagcag
     6241 ccaccgttcg ttctggtctt ttcatcgatt gggttcgaga cccgggaagt gcaggtcgcc
     6301 ggtgcgtcca atgctattac ggtggatctg gccgctgcca gcacgctggg ccgggaggtg
     6361 gtggtatcgg ccacccgcgg cgccatacgc agtcttgaat cgccggtctc tatcgagagg
     6421 atgggggcga ctacggcgca tgaagtac
//
LOCUS       DASYSV010000022         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_132885, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000022 DASYSV010000000
VERSION     DASYSV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132885"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1819
                     /locus_tag="VGQ51_00625"
     CDS             <1..1819
                     /locus_tag="VGQ51_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9035082.1"
                     /translation="YDLLIPYSNATLVFNPRMLHSWGNVFNETYVLLHSSAQKARLEA
                     GFPSLLPPHPDEEYNLYLQPLTSIHLDNTLPAGIQPVSNPKYSYILATIGLLILLVAC
                     VNFITLSVGRSTSRAIEVGVRKVLGAERRQLMRQFWGEALLLTLFSTMIGLLLTVVLL
                     HPFNQLTDRHLPMHFDPLFVLFCIGLLAIIALAAGIYPAIILSGFNPAEVLKGRLRLK
                     DKAGLFRKGLVVGQFLVSIVMIICTAIIAEQMHYLHYKDLGYNKDQVIIVPTNKGRAE
                     GFRLARLYEAELSKDPRVLSASASTFSFAETPWANLGYTDEQKSYHSFMYNEIDRSFL
                     KTMHIPIASGRGFEIENPGDSNSSILVNEALIKEYGLKDPIGRKFGIYSQRIVGVVKD
                     FNYESLHERIKPLVLSLKFDTIARQSSDLSYAVAPQPRISIRMKAGDMQENIALLKKA
                     WKAVAPQQDFEYRFLDQRLASAYAQEEKSSTVVRIASGLSIFIACMGLFGLVTLTITR
                     RTKELGIRKVLGANPAQLVRLLSRDFLLLVTIAAAIAFPIAWWLMRSWLSDFAYRTDI
                     SWWIFPTAALAAIFIVLVTIGIQTIRAALSNPIDALHTE"
     gene            complement(1855..3405)
                     /locus_tag="VGQ51_00630"
     CDS             complement(1855..3405)
                     /locus_tag="VGQ51_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035083.1"
                     /translation="MPNRSSDTLFQLIKSLEKSEKRNFKLYIKRNSSSENLKSIQLFD
                     AMDKMDDYDEAQLLNKNKNLRKQQLSNAKAHLYREILSSLRLIRQEENIDIQLHEQLD
                     FARILYNKGLYLQSLRTLDRIKEMARSKNQLTFLLQILFFEKTIESLHITRSMQDRAD
                     QLAGELDEVNDRLEIIGQLSNLSLELYSWYIRNGVARNENDEVAVQEFFERNMPANPS
                     QGQGFYEKLYQYQSFSWYAYIRQDFLLYYRYTQKWVDLFEKEPYMLAVETSQYIKGMH
                     NLLGAHFDLLNEQKFSETLKQFEAFSESDIVRNNVNNRIQTFVYLYISKINQHFIDGT
                     FTEGLALVPYIEEKLKEYWIYLDRHRILVFYYKIACLYFGSGDYEATIDYLNKIINWK
                     VDLRTDLQCYSRLLHLIAHYELGNFQLLEYLIKSVYRFMAKMGNLSRVEEEIFKFLRK
                     SFNLSPDLVKPEFGRLLERLQGYEKNRFETRAFMYLDIISWLESKIRQVPVQDVIRDK
                     FLSGMRRG"
BASE COUNT          873 a         1065 c          757 g          893 t
ORIGIN      
        1 atacgacctc ctgatcccct acagcaacgc aaccttagtg ttcaatccac gcatgttgca
       61 cagctggggc aatgtattca atgagaccta tgtccttctt cacagctccg cccaaaaggc
      121 cagactggaa gccggatttc catccctcct cccgccacat ccagacgaag aatataatct
      181 ttacctccag ccgctcacct ccatccatct cgacaacacc ctgccggccg gcatccagcc
      241 ggtcagtaac cccaaatact cttatatcct ggctactatc ggtctcctca tcctgctggt
      301 cgcctgtgtc aatttcatca ccctttccgt cggccgctcc acatcgaggg ccatcgaagt
      361 cggggtccga aaggtcctcg gggccgaacg acgtcagctg atgcggcaat tctggggaga
      421 agccctgctg ctcacgctct tttccacgat gatcggcctt ctcctcaccg tcgtgctgct
      481 ccatccgttc aatcagctta ccgacagaca tctcccgatg catttcgatc cgcttttcgt
      541 gctgttctgc atcggactcc tggcaatcat cgccctggcc gcaggcatct atcccgcgat
      601 catcctctcc ggtttcaacc ccgccgaagt gcttaaaggc cggctccgcc tgaaagataa
      661 ggcaggcttg ttcagaaaag gactggtggt cggccagttc ctggtttcca tcgtaatgat
      721 catttgcacg gccattatcg ccgaacaaat gcactatctg cactataaag acctgggcta
      781 taacaaagac caggtgatca tcgtgcccac caacaagggt cgggcggaag gcttccgcct
      841 ggcccggctc tacgaggccg aattgtcgaa agacccgcgc gtgctgtccg ccagcgcatc
      901 taccttcagc tttgccgaaa caccgtgggc caatctggga tatacggatg aacagaagag
      961 ctaccacagc tttatgtata acgaaataga ccgctcattc ctaaagacga tgcatatccc
     1021 cattgcatcc ggcagaggct ttgagatcga gaacccggga gactccaaca gcagtatatt
     1081 ggtgaacgaa gccctcataa aagaatacgg tttaaaagac cctatcggac ggaaattcgg
     1141 catctattcc caacggatcg tcggggtagt gaaagacttt aactacgaat cccttcatga
     1201 acggataaaa cccctcgtcc tctcgctgaa attcgatacg atcgcccggc agtccagcga
     1261 tctgtcctac gctgtcgcac cacaaccccg gatctccatc cgcatgaaag ccggcgatat
     1321 gcaggaaaat atcgcccttc tcaaaaaagc ctggaaagcg gtcgcccctc aacaggattt
     1381 cgaataccgt ttcctggatc agcgattggc ctcagcctat gcacaggaag aaaaaagctc
     1441 cacggtcgta aggatcgcgt ccggcctgtc gatcttcatt gcctgtatgg gcctgttcgg
     1501 gctggtaaca ctgaccatca cccgacgtac gaaagaactg ggcatccgga aagtattggg
     1561 cgcaaatccg gcacagcttg tccgactcct ctcccgcgat ttcctcctgc tcgttaccat
     1621 agccgcagcg atcgccttcc ccattgcctg gtggctcatg cggtcctggc tctcggattt
     1681 tgcctaccgt acggacatca gctggtggat cttccccacc gccgcgctgg ccgctatctt
     1741 tatcgtgctg gtgaccatcg gcatccagac catccgcgcc gcactctcca accccattga
     1801 cgctttgcac acggaataac ggcggcaggc cgcattatcc cacggtaagt cccgtcatcc
     1861 cctcctcatt ccacttaaga atttatcccg gatcacatct tgcaccggca cctgccgtat
     1921 cttgctctcc aaccacgaaa taatgtcgag atacatgaac gccctcgtct cgaacctgtt
     1981 cttctcatac ccctgcagcc tctccagcaa tcgcccgaac tccggtttca caaggtccgg
     2041 cgacagatta aaggacttcc tcaaaaactt aaatatctcc tcctccaccc ggctcagatt
     2101 acccatcttc gccatgaacc tgtacaccga tttgatcaaa tactccagca gctgaaaatt
     2161 acccagttcg taatgtgcga tcaaatgcag caaccgcgaa taacattgca ggtcggtccg
     2221 cagatccacc ttccagttga tgatcttatt cagataatct atcgtcgcct cataatcccc
     2281 gctcccgaaa tagagacagg ctatcttgta atagaaaacc aggatcctgt gccggtccag
     2341 gtagatccaa tactccttca acttctcctc gatgtaagga accagcgcca acccctccgt
     2401 gaacgtcccg tcgataaaat gctgatttat cttcgaaata tacaggtaca caaaagtctg
     2461 gatccggttg ttcacattat tgcggacgat atccgactct gaaaaagctt cgaattgctt
     2521 aagtgtctca ctgaacttct gttcgttgag cagatcgaaa tgcgcaccca gcagattatg
     2581 catgcccttg atatattgcg aggtctccac cgccagcata tacggctcct tctcgaaaag
     2641 atccacccac ttctgcgtat accggtaata gagcaggaaa tcctgccgga tataagcata
     2701 ccaggaaaaa ctctggtatt gatacaactt ctcataaaat ccctgaccct gactcggatt
     2761 ggccggcata ttcctctcga agaactcctg aacggctacc tcatcgttct cgtttcgcgc
     2821 aacgccgttt cggatatacc agctgtatag ctccagcgac aaattggaca gctgaccgat
     2881 gatctcaagc cggtcattca cttcatccaa ttcgcctgcc agctgatccg ccctgtcctg
     2941 catgcttcgc gtaatatgca aggactcgat ggtcttttcg aagaacagta tctgaaggag
     3001 aaacgtcagc tgattcttcg aacgggccat ttccttgatc cggtcaagag tgcggagact
     3061 ctgcagataa agtcctttat tatagaggat gcgcgcaaaa tccagctgct catgtaattg
     3121 aatatcaatg ttttcttcct gcctgatcaa tcgcaggctg gacaggatct cccgatacag
     3181 gtgtgccttg gcgtttgaaa gttgttgctt gcgtaaattt ttgtttttat ttaacaattg
     3241 cgcctcatcg taatcatcca tcttgtccat cgcgtcgaaa agctggatac tcttcagatt
     3301 ctcactgctg gaatttcgct tgatatatag cttgaaattc cgtttttcgg atttttccag
     3361 agattttatc agttggaaca gagtatctga cgatcggttg ggcatcgtat tttaaaattg
     3421 taaaaccctt tcctgtagcg ggtttcggcc cgtttacccc ccattaaggt ccgtaaaaag
     3481 accggtatta tgattttaag gcgaatttaa acaaagctaa atttattctc cttataatac
     3541 tatagctcat gactgaccaa cgaatcttca tcttcgacac taccctcc
//
LOCUS       DASYSV010000023         5414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1029802, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000023 DASYSV010000000
VERSION     DASYSV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029802"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VGQ51_00635"
     CDS             <1..765
                     /locus_tag="VGQ51_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV9035084.1"
                     /translation="LLHSTSRRWPNGLGNDNGLLGKYVAFQNYRGSLGGTVDGFEDKY
                     YYGRRPSECMMANFRNLKKQDTDFVGGYMAFMGAGRGQAEGGALPQQIGADYKKAICE
                     PGAWWMYIYMQGETVPKETNTVSLHPTEKDQWGIPLLTTKVDYDDNDERLLKDFMTQS
                     AEMLDTIGVKNITQHDNHWAPGRDIHEMGGCRMGKDPKTSLLNGHNQLHGCKNVFVTD
                     GACMTSTGNQSPSILYMAFTARAANYAVSEMKKGNL"
     gene            1301..2212
                     /locus_tag="VGQ51_00640"
     CDS             1301..2212
                     /locus_tag="VGQ51_00640"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HEV9035085.1"
                     /translation="MPTTPIREIRAFNRWYTDLIGLLNKNLLDSEYSLTEARLLFEIN
                     ATGTVRASQLMTILHIDKSYLSRLLRGLETAKLITCKRSSEDARAVLLSLSEKGRRVF
                     AVLNGASDQQVRRLLQPLNREQRLRLVGHMYGVRQLIEPDAPAEVTIRTMLQPGDLGY
                     IAYLHGRIYDQESAYGIGFESYVLKGLGELGHQYDPQKDGVWICENSGRIVGFLAGVS
                     RGASLQLRYFILLPEYRGKGLGRQLMDQFMEFLRDRGYRHAFLWTTDEQHAAIALYTR
                     YGFRLTEEKRSEAFGKPLTERKYEMNL"
     gene            complement(2207..3421)
                     /locus_tag="VGQ51_00645"
     CDS             complement(2207..3421)
                     /locus_tag="VGQ51_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEV9035086.1"
                     /translation="MNTLTKMAGRLFVFVLLSGIIRPARAQRKEVLPADRFLTAVPFM
                     VLNGGIVVGKVRLDSFPDSLNFIFDTGCGGVSLDSTTAVRYHLKPRASSLFIRGIGGE
                     LPQRLLEGRSLSVGGITLDSLTMQVNDYDLLSSVYGIKVDGIAGYSFYSRYVVRIDYD
                     SSMMYIYTKGPVKYPKGGYLLHPRLFGLPMLEGRLADARDISDRFYFDTGAGLCLLFS
                     SSFTSDSAVFAPRRKKPLPSQGAGLGGKAEMQLTTLRNFSLGPFHFRKIPTYIFNDDY
                     DVTSYPQLGGLIGNDLLRRFNLIVNYAHSEIYILPNSSYNQPFDYSYTGVYIGLFDGK
                     IEVTDVMTGSPAEKAGLKEGDIILMINGDAKQDVQVYQNLLRTFGPKVKVLVRHPNGE
                     LALLEMKVSSIL"
     gene            3570..5075
                     /locus_tag="VGQ51_00650"
     CDS             3570..5075
                     /locus_tag="VGQ51_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035087.1"
                     /translation="MPTDEILIRQIKRLYEEKTGWGDSGQWSNQDFLQLSEMIREQTG
                     VTISHVTLKRIWGKVRYESLPNTHTLNTLVQFVGYDSWRDFSVRNAGLLSPTPSANGD
                     HPSPSDNRDPSAPTSHGHTAGSTTSSANGHKTGSTALSSNGRPSVPGTDSRPSVPGAD
                     SRPSAATTNGHAAATGSAGAATRPMRMHVRRLRKIGWILIPLVVIMGVLLYLHGQQPS
                     PLSQDYAFSSKKVVTSGVPNSVVFDYDASRSPTDSVVIQQSWDTTRRVKVSKNGHRYT
                     SVYYYPDFFHATLQVQGKVVKTHNLLIETNGWVPIVHHEPVPVYFTPQDVIRDGKMSL
                     SADKIKEKNIPMQPTPPSVWFCNVHDFGEIYSDHFVFETALKNDYSEGSAICQVTKIY
                     ILCQGTAIWVPLCAKGCISDLDLYFTYFYTSGKRDDLSALGVDFSKYVKLRIESDSGR
                     AHIFVNDHLAYTVPRHILHSKIIGIDFNFQGTGSVDYVSLTNGKVDYRDDF"
     gene            5093..>5414
                     /locus_tag="VGQ51_00655"
     CDS             5093..>5414
                     /locus_tag="VGQ51_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035088.1"
                     /translation="MPKQILLILVLLLAAANAPAQVYLDAALDSLENMHLVTQLEKAK
                     LLERAVPLEKSNDYSRPDELLGLLGRAKMFSTSGTDNGFLFMAAPASSRSLPAPAIRA
                     ELDSF"
BASE COUNT         1338 a         1485 c         1389 g         1202 t
ORIGIN      
        1 ttgcttcatt ccaccagccg ccgctggccc aatggtctcg gcaatgacaa tggtctgctg
       61 gggaaatatg tagcctttca aaactatcgc ggttcgctcg gcggcaccgt cgatgggttc
      121 gaagacaaat attattatgg ccgccggcct tccgaatgta tgatggccaa tttccgcaat
      181 ctgaaaaaac aggataccga ttttgtgggc ggctatatgg cctttatggg cgcagggcgc
      241 ggccaggccg aaggaggtgc gttgccacag cagatcggcg cggactacaa aaaggccatt
      301 tgtgaacccg gcgcctggtg gatgtacatc tacatgcagg gtgagacggt tccgaaggaa
      361 acgaatacag taagccttca tcccaccgaa aaggatcaat ggggcatccc gctgctgacg
      421 acgaaagtgg actacgacga caacgatgag cggcttttga aggactttat gacccaaagc
      481 gccgaaatgc tcgacaccat cggcgtaaaa aatatcaccc agcacgacaa tcattgggcg
      541 cccggccggg atatccatga gatgggggga tgccggatgg gtaaggaccc taagacctcg
      601 ctgctaaatg gccataacca attacacggc tgcaagaatg ttttcgtcac cgatggcgcc
      661 tgtatgacca gtaccggcaa ccagagtcca tcgatattat atatggcgtt taccgcgcgg
      721 gcggctaatt atgccgtcag cgagatgaaa aaagggaatc tttgagatga tcaggccgca
      781 ttgttgcggg tgatccgaac gcatacgaaa cactttagga tgaagttgca aaaaacaagg
      841 tcgataaaat aacttttagt atcggtcttc atgtggtatt gacgccctac aaaagcaagc
      901 cctttgccta gctccagaag gaattcctgc agattggaaa taatggcggt ttcaagctgg
      961 ttctcggaat attcatttgg gcgagggatg tttcaggaat tcaagaacat agggatcctt
     1021 gatcatttca tgtggagcaa ttttttccag ctgttctttc tgcgttgcac tttctttgct
     1081 tttcctcgta gcctatttcg ttttccggaa ataccaaata gaattgcccg aaggatctta
     1141 gaaacatcgt cgtaaaaatg agataagggt ttcatagact cacagttagt tctaataaat
     1201 tactaaataa attaactaaa aactaaaaaa cttaggcatc ggggaaagat ttttaggctt
     1261 gatcgtttag ttttaattta gtggacaaag tccaccaatc atgcccacca cccccatccg
     1321 ggaaattcgc gccttcaatc gctggtatac agatctcatc gggctgctga acaagaacct
     1381 cctcgacagc gagtattcgc ttaccgaagc ccgtcttctt ttcgagatca atgctacagg
     1441 cactgtccgc gcatcgcagt tgatgaccat cctccacatc gacaaaagct acctgagccg
     1501 cctgttgcgg ggactggaaa cggcaaagct cattacgtgc aaacggtcgt ccgaagacgc
     1561 gagagccgta ttgttgtcac tttctgaaaa aggccgccgg gtattcgcgg tactgaatgg
     1621 cgccagcgac cagcaggtca ggcggctgct gcagccgctc aaccgtgagc aacgtcttcg
     1681 cctggtagga catatgtatg gcgtccggca gctgatagag cccgacgctc ccgctgaagt
     1741 cactatccgc accatgctgc aacccggcga tctcggctat atcgcctatc tccacggccg
     1801 catctacgac caggaatcag cctatggcat cggcttcgaa agctatgtgc taaaaggtct
     1861 cggcgaattg ggtcaccagt atgaccctca aaaagacggc gtgtggatat gcgagaacag
     1921 tgggcggatc gtgggctttc tcgcgggcgt cagccgcggc gcgagtctcc agcttcgata
     1981 ttttatattg ctgccggaat atcgcgggaa agggttgggc agacagctga tggatcaatt
     2041 catggaattt ctgcgtgatc gcgggtaccg ccatgccttc ctatggacca ccgacgaaca
     2101 acatgcagct atcgcattat atacccggta tgggttccgg ctgacggagg aaaagagatc
     2161 ggaggcattc gggaaacctc tgaccgaaag aaaatatgag atgaatctat aatatactgc
     2221 tgaccttcat ctccaacagc gccaactcgc cgttcggatg ccgcaccagc acttttacct
     2281 tgggcccgaa agtcctcaag aggttttgat atacctgtac gtcctgtttg gcgtcgccat
     2341 ttatcatcag aatgatgtcc ccctccttca accccgcctt ctccgccggc gaacccgtca
     2401 tgacgtccgt gacttcgatc tttccatcga ataacccgat atacaccccc gtgtagctgt
     2461 aatcaaacgg ctgattatag ctggagttcg gcagaatata aatttccgaa tgcgcataat
     2521 tgacgatcag attgaaccga cgcaacagat cgttcccgat cagccctcca agctgcggat
     2581 agctggttac atcgtaatcg tcgttgaaga tatacgtcgg tatcttccgg aaatggaaag
     2641 gccccagcga aaaattcctc agagtggtca gctgcatttc ggccttcccc ccaagacctg
     2701 ctccctgcga gggtaagggc tttttccgtc gcggagcgaa gaccgcgctg tcggatgtga
     2761 agctggaaga gaacaataaa cagagccccg caccggtatc gaaatagaac cggtcgctga
     2821 tatcgcgggc atcggccagc cggccttcga gcatgggtaa tccgaaaagc cggggatgca
     2881 gcagataacc acccttggga tatttgacgg gtcccttcgt atagatgtac atcatcgagc
     2941 tgtcatagtc gatcctcacc acgtaacgcg aataaaaact atacccggcg atcccgtcga
     3001 ccttgatgcc atagacgctg ctcagcagat cgtagtcatt tacctgcatg gtcagactgt
     3061 ccagcgtgat ccctccgacg gagagacttc tcccttccag caaccgttga ggcaattctc
     3121 cgccgatgcc ccggatgaac agtgaactgg ccctcggttt caggtgataa cggaccgccg
     3181 tcgtcgaatc gagcgaaacg ccgccacagc cggtgtcgaa aataaaattc agtgaatccg
     3241 gaaagctgtc gagtcgtact tttcctacca cgataccgcc attgagcacc ataaagggga
     3301 cagcagtcag aaagcgatcg gctggaagaa cttcttttcg ttgggcgcgg gcgggtcgga
     3361 tgatccctga cagaaggacg aatacaaaca ggcgacccgc cattttggtt agcgtattca
     3421 tcgtattatt gttaccttgt ctaaaattgg gagatacccc tggaaaaagc gaatcatttc
     3481 cgatacatgt tggtacaagt tggtacaaat caggggcgga ggacgaactc tcctaatttt
     3541 atgccatcca cattcttatt catttagtta tgcctacgga tgagatcctg atccgacaaa
     3601 tcaagcgact gtacgaggaa aaaaccggct ggggcgacag cggacaatgg agcaaccagg
     3661 attttttgca gttgagcgag atgatccggg aacaaaccgg ggtcaccatc agccatgtca
     3721 ccctcaaacg catctgggga aaggttcggt atgaaagtct cccgaacacc cataccctga
     3781 atacgctggt acaattcgtg ggatatgaca gctggcggga tttcagcgtc cggaatgcgg
     3841 ggctgctctc cccgacccct tccgcaaacg gagaccatcc gtccccatct gataacaggg
     3901 atccttcggc ccccacatcc catggccaca cagccggctc aaccacctca tccgccaacg
     3961 gccacaagac cggctcaacc gcgttatcct ccaacggtcg tccttccgtc ccgggcaccg
     4021 acagtcgtcc ttccgtcccg ggcgccgaca gccgtccttc cgccgcgacc accaatgggc
     4081 acgctgcggc aaccgggagt gcgggcgctg ccacccggcc tatgagaatg cacgtccgcc
     4141 ggctgagaaa gatcggctgg atcctcatcc cactggtcgt gatcatggga gtgctgctat
     4201 acctgcatgg ccagcagccc tcgccactgt cgcaggatta tgcgttcagc agcaaaaagg
     4261 tggtgacctc gggtgttccc aattcggtgg tgttcgatta tgacgccagc cgctcgccca
     4321 ccgattcggt cgtcatacag cagtcctggg acacgacccg gcgggtaaag gtgtcgaaga
     4381 atggtcatcg gtatacctcc gtctattatt acccggattt tttccatgcc actctgcagg
     4441 tgcagggaaa ggtggtgaaa acgcacaacc tgctgatcga aaccaacggc tgggtgccca
     4501 tcgtacacca tgagccggtg ccggtgtatt ttacgccgca ggatgtcatc agggacggta
     4561 agatgagtct gtccgccgac aagatcaaag aaaagaacat cccgatgcaa cctacgccgc
     4621 cttcggtatg gttctgcaat gtccacgatt tcggggagat atattccgat catttcgttt
     4681 tcgagacggc cctgaaaaac gactattcgg aaggatcggc catctgccag gtgacgaaaa
     4741 tatacatcct gtgccagggg acggccatct gggtgccgct gtgcgctaaa ggttgtatca
     4801 gcgatctgga tctgtatttc acctatttct ataccagcgg gaaacgcgac gatctctcgg
     4861 ccctgggcgt cgatttttcc aaatacgtca agctacgcat cgaatcggac agcggccggg
     4921 cccacatctt cgtaaacgac catctcgctt atacggtacc gagacatatc ctgcattcga
     4981 agatcatcgg catcgacttc aatttccagg gtacgggctc cgtcgattat gtttcgctaa
     5041 ccaacggcaa ggtcgattac cgcgatgatt tctgagagca acctaaacgc ctatgcccaa
     5101 acaaatcctt ctgatcctcg tcctgctgct tgccgccgcc aacgcacccg cgcaggtata
     5161 cctcgatgcc gcactggaca gcctggaaaa tatgcacctc gtcacgcagc tggaaaaagc
     5221 caaactgctg gaaagggcgg tgccccttga aaagagcaac gactattcgc ggccggacga
     5281 attgctgggt ttactgggac gcgcgaagat gttctccacc agcggcaccg acaacgggtt
     5341 cttattcatg gcagccccgg cttcctcgag atcgctcccc gccccggcca tccgcgccga
     5401 gctcgacagc ttta
//
LOCUS       DASYSV010000024         6893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2438009, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000024 DASYSV010000000
VERSION     DASYSV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2438009"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VGQ51_00660"
     CDS             <1..1129
                     /locus_tag="VGQ51_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HEV9035089.1"
                     /translation="WGITGQQDGIGDYDYYAYYYLSDSNAAYQFGNNYFQGWRPAGFY
                     STRRWEQTSTSNIGLDYGFLDNRIYGSIDFYHKNTTQLLNQVSQSALSNYSAYIVANV
                     GSMTNDGVEFSINLEPVRTRNVTWDLGFNITYNKNKITNLTVVPDDPNYPGFGSGNIA
                     GGIGGQFTEINAVGTPKNTFYLYRQVYDTKGNPIEGVFVDKNADGLINQNDLYKTKSA
                     DPNTFIGFSTSVNWGKWNFSTVLRSSLGMYDYNNIRSQTGTLNQVVKNVTLYNASTSV
                     LTTHFNGNAQELLSDYYISNASFLRMDNLSVGYNFGAISHTHTNLRLNAGVQNVFVVT
                     KYKGLDPEIGAGTDIGVGNPGIDNNAYPRPRVYNLGINLDF"
     gene            1161..2768
                     /locus_tag="VGQ51_00665"
     CDS             1161..2768
                     /locus_tag="VGQ51_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9035090.1"
                     /translation="MNNKIFTAAALSALCLLQAACSKDLNRVPNNGVTASQVYSTPLG
                     YTQALAKVYAAFANTGNVGGTGSPDLPTQIINDEGNSDFLRLYFNLQELTTDEAAWTW
                     ESDAGIQGLHEMSWSSTNPIVDGLYYRAAFQITLCNDFISHSSDAELAGKGFSSTTVD
                     SIRHYRAEARFLRAFQYWVLMDLFANPPFATEKDPIGAFIPKQISRSDLFKYIESELK
                     GAADSGLVAPHQALVGHADQAAAWSLLARIYLNASVYTGTARYTDAITYCNKVIGAGY
                     TLHPDYTKLMLADNQNNTDENIFDIMYDATFIQNYGGTTYLVHGPAGIPNNVSGVNGS
                     WSGLRLTQDFINLFPDFSGATDHRAQFWTTGQTKNMTALYTSTAGFATFKFRNVTSTG
                     AADPHQDNGDNFASIKFPLFRLAEIYLIYAESVLRGGSGGDAATALNYINALRTRAYG
                     NTSGNISSGQLTTSFILDERGRELYYECFRRTDLIRYNEFTTNAYLWAWKGGVAGGTA
                     VDPKYNIFPIPSTDLSANPNLKQNKDY"
     gene            2786..3871
                     /locus_tag="VGQ51_00670"
     CDS             2786..3871
                     /locus_tag="VGQ51_00670"
                     /inference="COORDINATES: protein motif:HMM:NF025650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusE domain-containing protein"
                     /protein_id="HEV9035091.1"
                     /translation="MHKMFHACLVFGLLVLAVGCKRDIPNPALTSPSGVSGFAVNNNN
                     VVLSASSDSATVATFSWQSANYGVNIPVTYTLLIDQPSDTSGATGWANAIVTSIPNGK
                     LSSAWLGTDFNHILNLLQLPTGVASTVVVRLKQAVIQSNGDTSTVPNLNGILTMTVTP
                     YHVILIYPKLYVAGDFLTPQWTQINQPGWILASVLNNGIYEGYVNMPNAGNSFKLCTQ
                     LDWNGTNYGWGGTQTTISGSGSAGNCYNAGPGYFRVVADTKGLTIQYFPAKWTIAGDF
                     NGWNVTANPMTFDANTNQWTATGISLTAGQGWKFVGDPSWNTNLGVDSKGNLAYNGPN
                     IGVAKTGTYTITLDVSQGAGNYTYSVK"
     gene            3874..4932
                     /locus_tag="VGQ51_00675"
     CDS             3874..4932
                     /locus_tag="VGQ51_00675"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEV9035092.1"
                     /translation="MRVTFFFLAVTIFCACSKSGASNGGGGTKDTQTHPAADTPIFRG
                     ADISWVTQMEDSGFKFYNSAGTAMDCFQLMQSLGMNSIRLRVWVNPSNKYNSTADFVK
                     KAVRAHNLGMRVLVDFHYSDTWADPGHQAKPAAWSAMNFATLTSTVTSYTKSVLDTLK
                     TAGVIPSWVQIGNETDNGMLWPDGQASTNMANFAALIDAGYKAVKDVSDTIKVIVHIS
                     NGWNNSLFRWMFDGLTANGAQFDIIGMSLYPAYFSSPGNWQACNDSCAANMNDMVTRY
                     HKPVMVVEVGMPANQPTASESFITDLMHKVKAVPGGNGLGVLYWEPESYAPFLSYGLG
                     AFDNTGKPTVAMNAFSTN"
     gene            4943..>6893
                     /locus_tag="VGQ51_00680"
     CDS             4943..>6893
                     /locus_tag="VGQ51_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEV9035093.1"
                     /translation="MKALLLLLGLPLALSAQTPRAHTSTDHTSPPPLRAHSSLDQTTP
                     SPLRAHTSTDQTSPPPLRAHTSLDQGWKFHLGDAADPSKDFNFRTVSIFAKSGKAEGT
                     AIAADFNDRDWRDISLPHDWAVELPFEFSPDPDVMSHGYKPVGGHYPSTSIGWYRKHF
                     TVATSDSGRRFVVRFDGIFRDSKVWINGFYLCGNASGYSGFSCDITDYLRYGHDNVLV
                     VRVDASQYEGWYYEGAGIYRHVWLNSYNDVHIAEDGLFVHSTVASPASATVTVETTVA
                     NDKTVPATTSVSAFVTTRDGILIGRSPAQSITIGPNEQRTVVQTIPVARPHLWSPETP
                     YLYRAGATLRSGSAAPPSPAPVRTAASAPPSPAPVIDEQEIRIGIRTIRIDAGGLYVN
                     GVYTKIKGVNNHQDHAGVGTAMPDYLQYYRARLLKEMGCNASRTSHNPPTPEWLDACD
                     SLGLLVLDENRLLNSGEEYLDQFRRLVIRDRSRACVFLWSLGNEEGFVQTRPVGRTIA
                     ATLIARQKEWDPTRTCTYAADLPNVYRGINEVIPVRGFNYRIAAVDPYHADHPNQPII
                     GTEMGSTVTTRGIYQKDTVNGYVPDEDITYPWWANTAEQWWALAADKPWWLGGFVWTG
                     FDYRGEPTPFKWPNINSHFGVMDMCG"
BASE COUNT         1587 a         2085 c         1750 g         1471 t
ORIGIN      
        1 ttggggaatc acaggtcagc aggacggtat cggcgactat gattattacg cttactatta
       61 cctcagcgac tccaacgcgg cataccagtt cgggaacaac tattttcagg gatggcggcc
      121 ggccgggttc tattccaccc gcagatggga gcagacgagt acgtccaata tcggactcga
      181 ttacggtttc ctcgataacc gcatctacgg tagcatcgat ttctaccaca agaatacgac
      241 ccagctgctg aaccaggtgt cgcaatcggc gctcagtaat tactcggcct acatcgtagc
      301 gaacgtcggc tccatgacca acgatggtgt cgaattcagc atcaacctgg aacccgtccg
      361 tacccgtaat gtgacctggg atctcgggtt caatatcacc tataataaga acaagatcac
      421 caacttgacg gtggtgcccg acgatcccaa ttatcccggc tttggcagcg gcaatatcgc
      481 cggcggcatc ggcggacagt tcacggagat caatgccgtg gggacgccca agaatacgtt
      541 ctatctctat cgccaggtgt atgatactaa gggaaatccc atcgagggcg ttttcgtgga
      601 taagaatgct gacggtctga tcaaccagaa cgatctgtat aagacgaagt cggccgatcc
      661 caataccttc atcggattct cgaccagcgt caattggggt aagtggaatt tcagcacggt
      721 cctgcgcagc agcctgggta tgtatgatta caacaacatc cggagccaga ccggcacact
      781 taaccaggtc gtcaagaacg tcacgctcta caatgcttct accagcgtgc tcacgacgca
      841 ttttaacggc aatgcgcagg agctgttgag cgattattat atttccaatg cgtctttctt
      901 gcggatggat aacctctctg tcggttataa tttcggggcc atctcgcaca cgcataccaa
      961 cctgcggctg aatgcaggcg ttcaaaacgt attcgtcgtc acgaaatata aggggctcga
     1021 tccggagatc ggcgcaggta cggacatcgg agtcggtaat cccggtatcg ataacaacgc
     1081 ctatccccgc ccacgcgttt ataacctggg catcaacctg gatttttaaa cccgaccaac
     1141 actcataaat tttaaatagt atgaacaaca agatattcac cgccgccgcc ctcagtgctc
     1201 tatgtctgct gcaggcggcc tgttccaagg acctcaaccg tgtcccgaac aacggtgtca
     1261 cggccagcca ggtctatagt accccgctcg gctatacaca ggcgctggcc aaagtttatg
     1321 ccgccttcgc caataccggc aatgtcggcg gcaccggcag ccccgatcta cccacccaga
     1381 tcatcaatga tgaaggcaac tccgatttcc ttcgtctcta tttcaacctg caggagctaa
     1441 caacggacga ggccgcatgg acgtgggaaa gcgatgccgg catacagggt ctccatgaga
     1501 tgagctggtc ttccaccaac cctatcgtcg atggtttgta ctatcgcgcg gctttccaga
     1561 tcacgctctg caatgatttc atctcgcatt cttcggacgc tgagctggcc ggaaagggtt
     1621 tcagcagcac gacggtggat tctatcaggc actatcgcgc cgaagcccgc tttctgcgcg
     1681 cctttcagta ttgggtattg atggacctct tcgccaatcc acccttcgcc accgagaaag
     1741 acccgatcgg cgcctttatc cctaagcaga tcagccgcag cgacctgttc aagtatatcg
     1801 aatccgaact gaaaggcgcc gccgacagcg gtctcgtcgc cccgcaccag gcgctggtag
     1861 gtcacgccga ccaggcagcc gcgtggtcgt tgctggcacg gatatacctc aatgcatcag
     1921 tctataccgg aaccgcgcgt tataccgacg cgatcaccta ttgcaataag gtcatcggcg
     1981 cgggctatac cctccatcct gactacacga agctgatgct ggccgacaat cagaacaata
     2041 cagacgagaa catcttcgat atcatgtatg acgccacttt tatccagaac tatggcggaa
     2101 cgacctatct cgttcatggc cccgcaggga tacccaataa tgtgtctggc gtcaatggca
     2161 gttggagcgg tctgcggctt acccaggatt ttatcaacct tttcccggat tttagcggcg
     2221 caacggatca ccgcgcccaa ttctggacca ccggccagac gaagaatatg accgcactct
     2281 atacatcgac ggctgggttc gctaccttca aattccgcaa cgtaaccagc accggcgcgg
     2341 cagacccgca tcaggacaat ggcgataatt tcgcgagcat caagtttccg ctgttccgat
     2401 tggcagagat ctatctcatc tatgcggaat ccgtgcttcg aggcggctcg ggcggagatg
     2461 ctgccacggc gctcaactat atcaacgcgc ttcggacgcg cgcatatggc aacacttcgg
     2521 gaaatattag ttccggccag ctgactacca gcttcatcct cgatgaacgc ggtcgcgagc
     2581 tatactatga atgtttccgc cgtacggacc tgattcgtta caacgaattt acgaccaacg
     2641 catatctctg ggcctggaag ggcggggtag caggcggtac cgccgtagac cccaaataca
     2701 atatcttccc gataccgagt accgatctgt cggcaaaccc caatctcaaa caaaacaaag
     2761 attactaatc ttcaatcata cgcatatgca caagatgttt cacgcatgcc tggtcttcgg
     2821 cctactggtc ctggccgttg gttgcaaacg ggacatacca aaccccgccc tgacctcgcc
     2881 ctcaggcgtg tcgggttttg cggtcaacaa caacaatgtg gttttgtcgg cctcgagcga
     2941 ctccgccacc gtcgcgactt tcagctggca gtcggccaat tatggggtga acatccccgt
     3001 tacctatacg ctgctgatcg atcagccatc cgacacgagc ggcgcgaccg gctgggccaa
     3061 tgccatcgtc accagcatcc cgaacggcaa actcagctcg gcctggctgg gtaccgactt
     3121 taaccacatt ctcaatctgc tgcagctgcc tacgggtgtg gccagcaccg tcgttgtccg
     3181 gctaaaacag gcggtcatac aaagcaatgg cgacacatct acggttccga acctcaatgg
     3241 tatattgacc atgaccgtta cgccgtatca tgtcatactc atttatccga agctgtacgt
     3301 agcgggagac tttctgaccc cgcaatggac ccagatcaac cagccgggct ggatcctggc
     3361 atcggtgctg aataacggca tctatgaagg atatgtcaat atgcccaatg caggcaatag
     3421 tttcaagctc tgtacacagc tggattggaa tggcaccaac tatggctggg gcggtaccca
     3481 gacaacgatc agcggcagcg gcagtgccgg caattgctac aacgcaggtc cgggttattt
     3541 ccgcgtggtg gccgatacca agggactcac catccaatat ttccctgcca aatggacgat
     3601 tgcgggagat ttcaacggct ggaacgtgac cgccaacccg atgaccttcg atgccaatac
     3661 caaccagtgg acggccacgg gcataagcct tacggcgggc cagggatgga aattcgtcgg
     3721 cgaccccagc tggaatacca atttaggcgt agattcgaag gggaatctgg cctataacgg
     3781 acccaatatt ggcgtagcta agacagggac gtataccatt acgctcgatg tgagccaggg
     3841 tgcgggcaac tatacttatt ccgtcaaata actatgagag tcactttttt tttcctggcc
     3901 gtgacaatct tctgcgcctg ctcgaaaagc ggtgcatcca atgggggcgg cgggaccaaa
     3961 gacacacaga cccatcccgc cgccgacacc ccgatcttca ggggtgcgga tatcagttgg
     4021 gtcacccaga tggaggactc gggctttaaa ttttataact cggccgggac ggccatggat
     4081 tgctttcagc tgatgcagtc gctgggcatg aattccattc gtctgcgcgt atgggtcaac
     4141 ccttcgaata aatataacag cacagcggac ttcgtcaaaa aggcggttcg cgcgcacaat
     4201 ctgggtatgc gcgtgctcgt cgacttccac tatagcgata catgggccga tccgggacat
     4261 caggccaaac ccgccgcctg gagtgccatg aattttgcca cgctgaccag caccgtcacg
     4321 agctatacga aaagcgtact cgacacgctg aagaccgccg gggtcatacc ctcctgggtg
     4381 cagatcggta atgagacgga taatggtatg ctctggcccg acggacaggc ctccacgaat
     4441 atggccaatt tcgcggcatt gatcgatgca ggatataaag ccgtcaagga cgtcagcgac
     4501 acgattaaag ttatcgttca tatctccaat gggtggaaca attcgctgtt ccgatggatg
     4561 ttcgacggac tcaccgccaa tggcgcgcaa ttcgacatca tcggcatgtc gctctacccc
     4621 gcctattttt cttcgcctgg caactggcag gcctgcaatg attcctgtgc ggccaatatg
     4681 aacgatatgg tgacgcgata tcataagcct gtaatggtcg tggaagtagg gatgccggcg
     4741 aaccagccga cggccagcga atcctttatc acggacctca tgcataaggt gaaggccgta
     4801 cctggcggga atgggcttgg cgtgctttac tgggaacccg agagctatgc gcccttcctc
     4861 tcatacggat tgggcgcttt cgataatacg gggaaaccca ccgtcgctat gaatgcattt
     4921 tctactaact aaactaactc tggtgaaagc actcctctta cttcttggat taccactcgc
     4981 tctctccgcg cagacccccc gggcccacac ctcgaccgac cacaccagcc cgcctcctct
     5041 ccgggctcat tcctcgctcg accagaccac cccatctcct ctccgggccc acacctcgac
     5101 cgaccagacc agcccgcctc ctctccgcgc tcacacctcg ctcgaccagg gctggaaatt
     5161 ccacctcggc gatgccgccg acccctccaa agacttcaat ttccgcaccg tctccatctt
     5221 cgcgaaatcc ggtaaggccg aaggcaccgc catcgccgcc gacttcaacg accgcgattg
     5281 gcgcgacatc tcgcttccgc atgactgggc cgtcgagctg ccattcgaat tttcccccga
     5341 ccccgacgtc atgtcgcatg ggtataaacc cgtgggcgga cactatccgt ccacgagcat
     5401 aggctggtat cgcaaacatt ttaccgtggc cacatccgac tccggccggc ggttcgtcgt
     5461 ccgtttcgac ggtatctttc gcgacagcaa ggtctggatc aacggctttt atctttgcgg
     5521 caacgccagc gggtacagcg gattttcctg cgacattacc gactatctcc ggtatggaca
     5581 tgacaatgtc ctcgtggtcc gcgtggatgc gtcacagtat gaaggatggt attatgaagg
     5641 cgcgggcatc tatcggcatg tctggctgaa cagctacaat gacgtccaca tcgcggaaga
     5701 tggactcttc gtgcattcga ccgtggcctc acccgcatcc gctaccgtca ccgtcgagac
     5761 gacggtcgcc aatgacaaga ccgttcctgc caccacctca gtttccgctt tcgtgacaac
     5821 ccgggacggt atcctcatcg gcagaagccc cgcccagtcg atcaccattg gccccaacga
     5881 acaacgcacc gtcgtccaaa ccattcccgt cgcccgccca cacctctggt cccctgaaac
     5941 accttattta tatcgtgcag gcgccacctt gcgttcaggc tccgcggctc ccccttctcc
     6001 cgccccggtc cgcaccgctg cctcggcccc cccttctccc gccccggtca tagacgaaca
     6061 agagatccgc atcggtatcc ggacgatccg catcgacgcc ggcggtctat atgtcaatgg
     6121 cgtctatacc aagatcaaag gcgtcaacaa ccaccaggat catgccggcg tcggaacggc
     6181 catgcccgac tatctgcaat attaccgcgc gcgtctgctg aaggaaatgg gctgcaacgc
     6241 cagccgtaca agccataatc cgccgacccc cgaatggctc gatgcctgtg acagtttggg
     6301 attgctggta ctggacgaga accgtctgct caatagcggc gaagaatatc tcgatcagtt
     6361 tcgccggctg gtgattcgcg atcggagtag ggcctgcgta ttcctctgga gcctgggtaa
     6421 tgaggaaggt ttcgtccaga caagacccgt aggccgcacc atcgccgcca cgctcatcgc
     6481 ccgtcaaaag gaatgggatc ccacgcgtac ctgcacctat gcggccgatc ttcccaatgt
     6541 ttatcggggt attaatgagg tgatacctgt gcgtggattc aactaccgta tcgccgccgt
     6601 ggacccctat catgcggatc atccgaacca acccatcata ggcaccgaga tgggcagcac
     6661 ggtaacgacc cgcggcatat atcagaaaga taccgtcaac ggctatgtgc cggacgaaga
     6721 tatcacctat ccctggtggg ccaatacggc cgaacaatgg tgggcgctcg ccgccgacaa
     6781 accctggtgg ctgggcggtt tcgtatggac gggtttcgac tatcgggggg aacctacgcc
     6841 attcaaatgg ccgaatatca actcgcattt cggcgtgatg gacatgtgcg ggt
//
LOCUS       DASYSV010000025         3652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2054021, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000025 DASYSV010000000
VERSION     DASYSV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054021"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VGQ51_00685"
     CDS             <1..449
                     /locus_tag="VGQ51_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV9035094.1"
                     /translation="SGFDVTILAYDRYKFGFAKDNVREASLEQLARYADVISFHVPLT
                     DETLHMAGDTFFASLEKKPFFINASRGKVHDTAALIRALESGKLAGAALDVLENEKLD
                     TYTPGQREQLKQLLARPDVLLTPHIAGYSHEAFLKMAQVTLEKLGI"
     gene            669..1142
                     /gene="greA"
                     /locus_tag="VGQ51_00690"
     CDS             669..1142
                     /gene="greA"
                     /locus_tag="VGQ51_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV9035095.1"
                     /translation="MSGFTYYTKDALDHLKAELQKLKTVERPAASKAIAEAREKGDLR
                     ENAEYDAAKEAQGLLEAKIQKLEGELVNARIIDQGAIDTSKVSILTKVTVTNLSNKKQ
                     MTYHLVSEKEADLKLGKISVTSPIGKGLLGKQVGDVANVTVPAGTMQFKIENITV"
     gene            1139..1528
                     /locus_tag="VGQ51_00695"
     CDS             1139..1528
                     /locus_tag="VGQ51_00695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV9035096.1"
                     /translation="MTIFSKIIAGEIPSYRIAENDRFYAFLDIFPLREGHTLVIPKLE
                     VDRFFDMPDPYLGEMLLFARPIAHAIEQAFDCNRCGLSVVGLEVPHAHLHLVPIDSSD
                     DLNFTRHKLRLKPEQLKAAQERILAHL"
     gene            complement(1557..2129)
                     /gene="ruvC"
                     /locus_tag="VGQ51_00700"
     CDS             complement(1557..2129)
                     /gene="ruvC"
                     /locus_tag="VGQ51_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEV9035097.1"
                     /translation="MQSKIILGIDPGTVVMGYGLIQCASDRLSLIEMGVLRLPARKSG
                     HERLRLIHAKVAELIGQFGPHDFAIEAPFFGKNVQSMLKLGRAQGVAIATAMHAGLPV
                     TEYSPKKVKQSITGNGNSEKEKVWLMLRRILSLEEELPSYDASDALAVAVCHYYQHRT
                     EVLFEGQRAKKKAGSWEDYVRQNPGKVREP"
     gene            2134..3123
                     /locus_tag="VGQ51_00705"
     CDS             2134..3123
                     /locus_tag="VGQ51_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEV9035098.1"
                     /translation="MNKSIKILVRYVLGPVVFLVLSWFIYWQVTRQHDWRTSLHQVLA
                     ALTGPQQWKIWLVVALMPLNWGLEARKWQLALRPVGGIAFKDALKAVFTGAAVAFFTP
                     NRMGEYLGRILYVKEGRRMKAIALTIVSSMAQLMITLAMGLLGVLFIRGSVHREIPGQ
                     LNWQLVLNILLGIVPIALLSLTFIYCRLTRITDWLLKIPGTRKYSAYIKVLESFDPTS
                     LRRIFFLSFVRYIVFIVQYSLVFPVFGVEVGLWQGIGGMSVVFLIMAVVPTMTFLTEL
                     GLRWEASIQVLELYSSNMVGIFATSFAIWLINLIIPAMIGSLLIVSIKFSKSR"
     gene            3120..>3652
                     /locus_tag="VGQ51_00710"
     CDS             3120..>3652
                     /locus_tag="VGQ51_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEV9035099.1"
                     /translation="MRVIITVCILLLTLTLPVMAWDAYDQQTDTAVQQTDQTHSEFCG
                     GIRNTAFKAGESLTYKVFYTVAGFHVAAAGEATFNTSFERFQGKDCYHVVAEGKSYPF
                     YDKFFRVRDKYESYIDTSTLQPYKFIRNVDEGGYKTYENVSFIKAAGTAVTNSGVYQV
                     PDCIQDVLSASFYARNI"
BASE COUNT          836 a         1005 c          924 g          887 t
ORIGIN      
        1 tctcgggttt cgatgtcacc atattggcct atgacagata caaattcggg tttgcgaaag
       61 acaatgtccg ggaggcctcg ctcgaacaac tcgcccgtta tgccgacgtc atcagcttcc
      121 atgtcccgct gacggacgag accttgcata tggccggcga caccttcttc gcttcattgg
      181 aaaaaaagcc cttctttatt aacgcttcac gggggaaggt ccacgatacc gcggcgctga
      241 tccgggcatt ggaatccgga aaactggccg gtgctgcgct cgatgtgctc gaaaacgaaa
      301 agctggacac ctatacaccc gggcaacgcg aacagctgaa gcaactgctg gcccggccgg
      361 acgtcctctt aactccacac atcgccggct attcccatga ggcttttctg aaaatggcgc
      421 aggtgaccct cgaaaaactg gggatctagg cctccgccgt tatccgggcc gtcgccattc
      481 tcatccgggc cctcgtcaat ttcccgggtg cggcgatccc cgggtttgcc gaaattggtt
      541 atttaggcca ttttcccgta aatttgctgg aaatgggcca acggtccatt ggaaaatatt
      601 tgtgcaatgc cttggatcgc tccagggcgt tgtatttttt ttcttcttgc cttcaaaaag
      661 gaggagttat gagtggattc acctactata ccaaagacgc cctcgaccat ctcaaggccg
      721 aattgcaaaa actgaagacg gtagagcgcc ccgccgcttc caaagccatc gccgaagccc
      781 gcgaaaaagg cgatctccgc gaaaacgcag aatacgatgc tgccaaagaa gcccagggtc
      841 tcctcgaagc caagatccag aaactggaag gtgaacttgt caacgcacgc atcatcgacc
      901 agggagccat cgacacgagc aaggtgtcga ttctgaccaa agtcacggtg accaaccttt
      961 ccaataaaaa gcaaatgacc tatcacctcg tgtcggaaaa agaagccgat ctgaagctgg
     1021 ggaaaatatc cgtcacttct cccatcggca aaggcctgct gggaaaacag gtaggagatg
     1081 tcgccaatgt cacggtaccc gccgggacca tgcagttcaa aatcgaaaac attacggtgt
     1141 gaccattttt tcgaagatca tcgcagggga gataccgtct taccgcattg cggagaacga
     1201 ccggttctat gctttcctcg atatctttcc tttacgggaa ggtcataccc ttgtcatccc
     1261 caagctggag gtagaccgct tcttcgatat gcccgaccct tatctgggag agatgctgct
     1321 ctttgcgcgc cccatcgcgc atgccatcga gcaggcattc gactgtaacc gctgcgggct
     1381 ttccgtggta ggtctcgagg tgccgcatgc tcatcttcac ctggtgccca tcgacagctc
     1441 cgacgatctg aatttcacac gccataagtt aagactgaaa cccgagcagc tgaaagctgc
     1501 ccaggagagg atcctcgccc atctctgacc gctctctttc gccgatcgcc gtttcctcac
     1561 ggctcccgga cttttcccgg gttctgccgc acataatcct cccagctccc cgccttcttt
     1621 tttgcccgct gaccttcgaa taatacctcc gtccgatgct gataataatg acataccgcc
     1681 acggccagcg catcgcttgc gtcataggac ggaagctctt cttccagcga aagtatccgc
     1741 ctcagcatca accatacctt ctctttttct gaatttccat tgccggtgat cgattgtttc
     1801 accttcttgg gagagtattc ggtgacggga agtccggcat gcatggcggt ggcgatcgcg
     1861 accccctgcg cccggcccag tttcagcata ctttgtacat tctttccgaa aaaaggtgcc
     1921 tcgatggcga agtcatgggg accgaactgt ccgatcagct ccgctacttt cgcatggatc
     1981 agtcgtaggc gttcatggcc gctcttcctg gccggcaacc gcaaaacgcc catttcgatc
     2041 aggcttaacc ggtcgctggc acattggatg agtccgtaac ccatcacaac cgttccgggg
     2101 tcgataccta aaattatttt agattgcatt gcgttgaaca aaagtataaa aatactggtt
     2161 aggtacgtgc ttgggccggt ggtattcctc gtactatcct ggttcatcta ttggcaggtg
     2221 acacgccaac acgactggcg gacgtcgctt catcaggtcc tggccgccct caccggtccg
     2281 cagcaatgga agatatggct ggtagtggcg ctaatgccgc tcaactgggg actggaggcc
     2341 cgtaaatggc agctggcctt gcgcccggtc ggaggcatcg cttttaagga tgcccttaaa
     2401 gccgttttta ccggggcggc cgtcgccttt tttacaccga acaggatggg ggaatacctg
     2461 ggccgcattc tctatgtaaa ggaaggccgc aggatgaagg ccatcgccct gacgatcgtt
     2521 tcgagcatgg cgcagctgat gatcaccctg gcgatgggat tgctgggtgt tcttttcatc
     2581 cgcggttcgg tacatagaga gatcccggga cagctcaact ggcagctcgt gctgaacatt
     2641 ctgttgggca tcgtaccgat cgcgctgcta agtttaacat ttatttattg ccggctgacc
     2701 cggatcacgg attggttgtt gaaaataccg ggcaccagga agtatagcgc atatataaaa
     2761 gttttagaaa gtttcgaccc tacgagctta aggcggattt tcttcctatc ttttgtcaga
     2821 tatattgttt ttattgtcca atatagtctg gtgtttcccg tttttggagt agaagttggc
     2881 ctttggcagg ggataggtgg catgagtgtt gtatttttga tcatggcggt cgtccccaca
     2941 atgacgttcc tgacggaatt aggattgaga tgggaagcca gtatccaggt gctggaattg
     3001 tatagttcca atatggtggg cattttcgcc acctcttttg cgatctggct gatcaacctc
     3061 atcataccgg cgatgatagg aagcttgtta attgtaagta tcaaattttc gaaaagtcga
     3121 tgagagtaat tattacggtc tgtatcttgt tgctgacgtt gaccctcccc gtaatggcat
     3181 gggatgccta tgaccagcaa accgacaccg cggtgcaaca gaccgatcaa acccacagcg
     3241 aattctgtgg cggaatccgc aacaccgcct tcaaagccgg ggaatccctc acttacaaag
     3301 tattttatac cgtggccggt ttccatgtcg ccgccgcggg cgaggcgacc ttcaatacca
     3361 gcttcgagcg cttccagggc aaggattgct atcacgtggt ggccgaagga aagagctatc
     3421 ctttctatga caaattcttc cgtgtccgcg acaaatacga gtcctatatc gacacctcca
     3481 cgctgcaacc ctataaattc atccgcaatg tcgatgaggg cggctacaag acgtacgaga
     3541 acgtctcctt catcaaggcg gccggcaccg cagtcaccaa ctccggtgtt taccaggtgc
     3601 ccgattgcat acaggacgtg ctgagcgcgt ctttctatgc acgcaacatt aa
//
LOCUS       DASYSV010000026         5473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1927576, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000026 DASYSV010000000
VERSION     DASYSV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927576"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /gene="bshC"
                     /locus_tag="VGQ51_00715"
     CDS             complement(<1..1158)
                     /gene="bshC"
                     /locus_tag="VGQ51_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEV9035100.1"
                     /translation="MDWVSTQLPYQQTGYFSRIITDYLEQAGELSSFYAHPVSPEGFR
                     AAIGARRQSPVDRKALVDALTEQYAGAEPAPKVSDNITRLGDDNTFTVCTAHQPAIFS
                     GHLYFIYKILHTIRLAEWLREQHPDKHFVPVFFMGSEDADLDELGHIYLGEEKLVWET
                     KQTGAVGRMNTKGLDQLVYRIEGELSVQPYGGQLIQLIKRAYLESPDIQTATFRLLHQ
                     LFGEYGLVVLIADKAVFKRQMISIFEDDLFLQGPSGIVDKTIARLSEQYKVQANPRPI
                     NLFYLKGDMRNRIERVGEMFAVVDSPLRFNEQELREELNRYPERFSPNVILRGLFQET
                     ILPNLAFIGGGGETAYWLELKDLFDHYRMPFPVLVLRNSFLLVQKAWMLRLE"
     gene            complement(1316..2317)
                     /locus_tag="VGQ51_00720"
     CDS             complement(1316..2317)
                     /locus_tag="VGQ51_00720"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HEV9035101.1"
                     /translation="MKKTFFCIDAHTCGNPVRLVAGGGPVLEGSNMSEKRQHFLKEFD
                     WIRKGLMFEPRGHDMMSGSILYPPHDPQNDVAVLFIETSGCLPMCGHGTIGTITIALE
                     EGLIQPKKPGHIRMEAPAGLVSIRYKQEGRKVSSVKLTNVPAYLAAENITVECPDLGP
                     LKMDVAYGGNFYAIVDPQENFRGLEDYSADQLIGWSRVLRQRINAQHTFVHPENPTIH
                     TCTHILWTGKTIDPGATARNAVFYGDKAIDRSPCGTGTSARMAQWFAKGRLKEGDIFV
                     HESIIGSKFTGRVEKAVTVAGKPAIIPSIEGWAKIYGYNTITIDPEDDPYAYGFQVL"
     gene            complement(2319..3794)
                     /locus_tag="VGQ51_00725"
     CDS             complement(2319..3794)
                     /locus_tag="VGQ51_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HEV9035102.1"
                     /translation="MKFQDATVQEIDAIMQEAWNAFLVFRRTLPKQRADLMRAIAREL
                     EATGDGLIQTAMRETNLPETRLRNERARTMFQLTSYAEACEAGHWLEARIDTQEKADP
                     DVRKLQVAVGPVVVFGASNFPFAYSTAGGDTASALAAGCPVVVKAHPAHAETSGLVAE
                     AIARAVAAAGLPKGVFGHVHGAGFETGKALVTHTHTRAVGFTGSFAGGKALFDWANQR
                     PVPIPVFAEMGSINPVFLLPGKLKNFARELAGQLAGSITLGTGQFCTNPGLILGVEGE
                     DLDLFIEELGVAIEKTTPGTMLHPGIARAYSEKRGTALSQANVNTVAIAATEPQANQG
                     VPTVASATAEAFFKNPILHQEVFGPYSLIIQCKNMDELEKAARHLEGQLTVSLMATDD
                     DLSRHASLVTAAQDIAGRLILNGVPTGVRVALAMQHGGPFPATTDSRFGAVGADGIKR
                     WVRPLCYQSWSDAALPEALQNSNPLGIWRTINNNLTTDAVK"
     gene            complement(3806..4723)
                     /locus_tag="VGQ51_00730"
     CDS             complement(3806..4723)
                     /locus_tag="VGQ51_00730"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV9035103.1"
                     /translation="MRINWNGVFPALTTKFDAHEELDLPMFGHNLAAQIDAGVNGVII
                     GGSLGEASTLTLGEKEKLVRYAVQSATNRIPVLLNIAEGSTREAVAQAQMGEKWGARG
                     LMVLPPMRYKSDKRETVAFFKTIASSTGLPIMIYNNPVDYKIEVTLDMFEELIDLPNI
                     QAVKESTRDVTNVTRLINRFGDRLNILCGVDTIALEELLMGAVGWVGGLVCAFPAETV
                     AIYRLAKAGRISEAREIHRWFLPLLELDIHAKLVQYIKLAEAATGLGTEYVRAPRLPL
                     VGTEREQVLQVIHEGVANRPLLTDLVMTA"
     gene            complement(4829..>5473)
                     /locus_tag="VGQ51_00735"
     CDS             complement(4829..>5473)
                     /locus_tag="VGQ51_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV9035104.1"
                     /translation="HYAYPSKFPVEILDVIRERENICNYLDIPFQHASDNMLKAMRRQ
                     VTKSETADLIRTIREKVPGICLRTTLIAGFPGETEEDVEELKAFLREQRFDRVGIFQY
                     SHEEGTSAYGLEDSIPAEEKQRRAQEVMEVQQEISFEKNQEKIGRVFRTLIDKKEAGR
                     YLGRTEFDSVEVDNEVIVHSAGKLPIGEFVNVRITKAYDYDLEGEVVVPDAEGK"
BASE COUNT         1134 a         1582 c         1473 g         1284 t
ORIGIN      
        1 ctccagccgg agcatccacg ccttttgaac cagcaaaaat gagtttcgca acaccagcac
       61 agggaacggc atccgatagt gatcgaacag gtctttcagc tcgagccagt acgcggtctc
      121 gccacctccc ccgataaagg ctaagttggg aaggatggtc tcctggaaca gaccgcgcag
      181 gatgacattg gggctaaagc gttcgggata acggttaagc tcttcacgca actcctgttc
      241 gttgaagcgt agcggggaat ccacgactgc gaacatctct cccacccgct cgatccggtt
      301 gcgcatatcg cccttcagat aaaagagatt gatgggtctc ggattggcct gaactttata
      361 ctgttcgctt agtcgcgcga tggtcttgtc tacgatgccg gaaggcccct gaagaaaaag
      421 gtcgtcctcg aaaatggaga tcatctgtcg tttgaatacc gccttgtccg cgatgagcac
      481 gaccaatcca tattccccga acagctggtg tagcagccgg aaggtcgccg tctggatatc
      541 ggggctttcg agataggcgc gtttgatcag ctgtataagc tgcccgccat aaggttgtac
      601 cgacagttcg ccttcgatgc gatagaccag ctggtcgaga cctttggtgt tcatacggcc
      661 cactgctccc gtttgtttcg tctcccagac cagcttctcc tcgccgagat agatatggcc
      721 cagctcgtcc agatcggcat cctcgctgcc catgaaaaag acgggcacga aatgtttgtc
      781 gggatgctgt tcacgaagcc attcggcgag acgaatggtg tgaaggatct tatagataaa
      841 ataaaggtga ccgctaaaga tagccggctg atgggcggtg cacacggtaa aggtgttgtc
      901 gtcacccagc cgggtgatat tgtccgacac tttcggcgct ggctctgctc cggcatattg
      961 ttctgtcaat gcatccacca acgcttttcg gtcgacggga gactgtcgtc tcgccccgat
     1021 agcggcgcgg aagccctcag ggctgacggg atgagcataa aaggaagaca attcgccggc
     1081 ctgttccagg taatccgtga tgatgcggga aaaatagccg gtctgctgat atggtaattg
     1141 cgttgatacc cagtccatga ttgtgcggat ggttggcgaa tatattatct ccggaggata
     1201 aaaacagcgc tcccggagga atttatccaa acggcattga accgccacgg gcggctcgtc
     1261 cggtaggaca gacattcgtg cacaacagag aaatggcgtc tttgttcagg acgagctaca
     1321 gcacctggaa tccgtaggca taaggatcgt cttccgggtc gatggttatc gtgttgtacc
     1381 cgtagatctt ggcccagcct tcgatgctgg ggataatggc cggtttgccg gcaaccgtaa
     1441 cggctttttc cacccggccc gtaaatttcg atccgatgat gctttcatgg acgaagatat
     1501 ctccttcttt cagacgccct tttgcgaacc attgcgccat ccgcgccgag gtgccggtcc
     1561 cgcaagggct ccggtcgatg gccttatccc cgtaaaaaac ggcattgcgc gcggtcgcgc
     1621 cgggatcgat ggtcttgccc gtccaaagga tatgcgtaca ggtgtggatc gtaggattct
     1681 ccgggtgcac gaaggtatgc tgcgcattga tccgctgacg caacacgcgg ctccagccga
     1741 tgagctggtc ggcggaatag tcttcgagtc ccctgaaatt ttcctgtggg tcgactatcg
     1801 catagaagtt cccgccatag gcgacgtcca tcttcaacgg tcccagatcg ggacattcga
     1861 cggtgatatt ttctgcggca agataggcgg ggacattcgt cagtttgacc gaactgacct
     1921 tcctgccttc ctgtttgtat ctgatcgata ccagaccggc aggcgcctcc atacggatat
     1981 gaccgggctt tttcggctgg atcaaccctt cttcgagcgc gatggtgatc gttccgatgg
     2041 taccatgtcc gcacatcggg agacaaccgc tggtctcgat gaaaagtacg gcgacgtcgt
     2101 tctgagggtc atgaggcgga tacaggatgc tgccgctcat catatcgtgt cctctcggct
     2161 cgaacatcag tcctttgcgg atccagtcga attcctttag aaaatgctgg cgcttttcgc
     2221 tcatgttact gccttcgagt acggggccgc cgccggcgac cagacggacg ggattaccgc
     2281 aggtatgggc atcgatacag aaaaaggtct tcttcatact atttgacagc gtctgtcgtc
     2341 aggttgttgt taatggttct ccagatgccc agcgggttgc tgttttgcaa ggcctccggc
     2401 agcgccgcgt ccgaccagct ctgatagcac agcggccgca cccagcgttt gataccgtcc
     2461 gctccgacgg cgccaaaacg gctgtcggta gtggctggga aaggtccccc gtgctgcatg
     2521 gctaaagcta ccctgacgcc cgtggggacg ccgttcagga tcaaccggcc tgcgatatcc
     2581 tgggcagccg tgaccagcga tgcgtgtcgg gaaagatcgt catcggtagc catcagggaa
     2641 acggtcagct gtccttcgag atgacgcgcg gccttttcca gctcgtccat attcttgcac
     2701 tggatgatca gcgaataggg gccgaagact tcctggtgaa ggatgggatt tttgaagaac
     2761 gcttccgcgg tagcggaagc taccgtaggc acgccctgat ttgcctgtgg ttccgtggcg
     2821 gcaatggcca cagtattgac attggcctgc gaaagcgcgg taccgcgttt ttccgaatag
     2881 gccctggcga tcccgggatg cagcatcgta cccggagtcg ttttttcgat cgcgactccc
     2941 agctcttcga taaaaagatc gagatcttct ccttcgacgc cgagtatgag acccgggttg
     3001 gtgcaaaatt gcccggtacc gagggtaatg gaacctgcca gttgacccgc cagctcgcgc
     3061 gcaaaattct ttaattttcc cgggagcagg aagacgggat ttatcgagcc catttccgca
     3121 aatacgggta taggcaccgg tcgttggttg gcccagtcga acagcgcctt gccgcctgcg
     3181 aacgatccgg taaagcctac tgcgcgggta tgggtatgcg ttaccagggc cttgccggtt
     3241 tcgaagccgg cgccatggac atgtccgaaa acacctttgg gaaggcccgc agccgcaacg
     3301 gcccgggcga tcgcctccgc caccagcccg gaggtttccg catgtgccgg atgagccttg
     3361 acgacgacgg gacatcctgc cgccagtgcc gacgcggtgt cgccaccggc cgtggaatag
     3421 gcaaatggga aattactcgc gccgaatacg accaccggtc cgaccgccac ctgcagcttc
     3481 cgcacatcgg ggtcggcttt ctcctgcgta tcgatccgcg cctccagcca gtgtcctgct
     3541 tcgcaggctt cggcatagct ggtcagctgg aacatcgtac gcgcccgttc gttacgcagc
     3601 cttgtctcgg ggagattggt ttcccgcatc gcagtctgga tgagaccgtc accggtcgct
     3661 tccagttcac gggcaatagc ccgcatcaga tcggcgcgct gtttcggcag ggtccgccgg
     3721 aagactaaaa aggcattcca ggcttcctgc atgatcgcgt ctatttcctg tacggttgca
     3781 tcttgaaatt tcatattgca aagttttagg ctgtcattac tagatccgtc aacagtggac
     3841 ggttggccac cccttcatgg atcacctgca acacctgttc ccgttccgta cccaccagcg
     3901 gcaaacgcgg tgcgcgcaca tattcggtgc ccaacccggt ggcagcttcg gccagtttga
     3961 tatactgtac cagtttggca tggatatcaa gctccagcaa gggcaggaac caccgatgga
     4021 tctcgcgggc ctccgagatc cgtcccgctt tcgccaggcg gtagatagcc actgtctcgg
     4081 ccgggaaggc acagaccagg ccgccaaccc aacctacggc gcccatcagc agctcttcaa
     4141 gtgcgatggt atccacgccg cacaggatgt tcagcctgtc tccaaaacgg ttgatcagac
     4201 gcgtgacatt ggttacgtcc cgcgtcgact ccttcactgc ctggatattg ggcagatcga
     4261 tcagctcttc gaacatgtcc agggtcactt ctattttata atcgaccggg ttgttgtaga
     4321 tcatgatcgg caatccggta gaagatgcga tggttttgaa aaaagcgacg gtctcgcgtt
     4381 tgtcgctctt ataccgcatg ggcggaagga ccatcagacc gcgcgccccc catttctctc
     4441 ccatttgtgc ctgcgccact gcttcccgtg tcgaaccctc ggcaatattc aacaggaccg
     4501 ggatccggtt tgttgccgat tgcaccgcgt agcggaccag cttttccttt tcacccaacg
     4561 tcagcgtgct ggcctcaccc agcgaccccc cgatgatcac tccgttcact ccggcatcga
     4621 tctgcgcggc cagattatgg ccgaacatcg gaagatcgag ctcctcatgg gcatcgaact
     4681 tcgtagtaag agccggaaag actccattcc aatttattct catgtaacca aattttcgac
     4741 aaatgtaccg gtttgacctc tttgtcagtc ggcgccgatt aatctgttct atgcagattt
     4801 taacttagca tcgccacttc ccccgccttc acttcccctc tgcatcaggg accaccactt
     4861 ccccctcgag atcgtaatca taggccttag tgatccgcac attgacgaat tcccctatcg
     4921 gcagtttccc ggcactatgg acgatcactt cattatccac ttctacagaa tcgaattccg
     4981 tgcgccccag ataacgaccc gcctcttttt tatcgatcag ggttcggaac acacgcccga
     5041 tcttctcctg gttcttctca aaggagatct cctgctgcac ttccatgacc tcctgtgccc
     5101 gtcgttgttt ttcttcagcg ggtatcgaat cctccaatcc atacgcggag gtgccttctt
     5161 cgtgcgaata ctggaagatg cccacccggt cgaaccgttg ttcgcggaga aatgccttca
     5221 gctcttcgac gtcttcttcc gtctcaccgg gaaaacccgc gatcagtgtc gtacgtaaac
     5281 aaatgcccgg aactttttcc cggatcgtcc tgatcaggtc cgccgtctcg gacttcgtca
     5341 cttgtctgcg catggccttc agcatattgt cgctcgcatg ctggaacggg atatcgagat
     5401 aattacagat attttccctt tcgcggataa catcgagtat ctctaccgga aatttagaag
     5461 ggtatgcata atg
//
LOCUS       DASYSV010000027         5568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_646513, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000027 DASYSV010000000
VERSION     DASYSV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646513"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2267
                     /locus_tag="VGQ51_00740"
     CDS             <1..2267
                     /locus_tag="VGQ51_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9035105.1"
                     /translation="FIFDEKSSYNGGVSAPIYQALRKEAVGLKRVVPVMGLYLRAVRS
                     NPADGKPVTVEDQDGIASTDAVYFSMVPYRWLAGQGGTALNAPNKVVLTRSRAQQYFP
                     GKTPQEILNSTLTYYSYRDTITRTVAGIVADPEGPTEFTTKEFCSLPEKEYSLNSWTN
                     TNGGDKLYLQLEDNAVPARVLATIERVVGEKVRQFEQTRKDNFKFKRWFELLPMSESH
                     FSTYINEYTVHKANKNVLYGLMGVGLFLLVLACINYINMGVASIPQRAKEIGVRKTLG
                     GRRSALIAQFLTETLLTTFLACLLAFALGWLEFRLLRDILPDKLEPWGGMGQVAVFMG
                     VLVILVTLLSGLYPGWLITKVKTVEVFRRVFLVKNRGGRIGLQKALIVFQFVIALVFI
                     TGAMIVGSQLHYVIDSDMGFNKDAVVLVDVPWKYSGSPLYKDKQYSLLAELKTLPGIR
                     GIALGDQPLESGYSSSGFNYYHDGKAPVSRQVFRKEIDTAYLGFYGFHLLAGRNLSPS
                     DTTNELVINETAVKAFGFASPQDALGKFIGQNATGLLPIVGVIKDFHTRDFYNTIDPM
                     AFMSDRGVSTFNIRLDNAHAADWQQTLKAIEKKWYGFYPPGSWSYKFYDETIAELYKN
                     ERHLSTLIDLTTGISIFISCLGLFGLAVLTAYQRTKEIGIRKVLGATVPGIVGLLSRE
                     YLRLVALAIIIAIPISWWAADKWLQNFAYKIPLSWWLFAAAGVSGLAIALVTVGFRAL
                     KAARVNPVNSLRIE"
     gene            2291..2809
                     /locus_tag="VGQ51_00745"
     CDS             2291..2809
                     /locus_tag="VGQ51_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HEV9035106.1"
                     /translation="MSVLKERFERNMPRHEGIQWAKVAARLESNPGKLRSLNEMERTG
                     GEPDVVGFDAHSGEYIFYDCSAESPKGRRSLCYDDAALESRKENKPDGSAMGMAAAMG
                     IGILTEEQYRGLQRLGKLDLKTSSWVLTPAAIRKLGGAVFCDRRYDTVFLYHNGAESY
                     YAARGFRGWVRV"
     gene            complement(2817..3701)
                     /locus_tag="VGQ51_00750"
     CDS             complement(2817..3701)
                     /locus_tag="VGQ51_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HEV9035107.1"
                     /translation="MRLLFLTYLCPMVHVGEYNTLKVKKELEFGLILDDGEGGILLPK
                     RFVPRGARIGDELNVFLYHDGEDRLIATTQRAKAILGDIALLRVVSVTPLGAFLDWGL
                     MKDLFVPKSKQLSGMRFGGDYLVKIYLDEKTGRLAATEKIEPFLSNDDITAKEKDLVD
                     LTIWRRSDIGYVVIINNRHTGVLHFNEVFSNIAIGDRFPGFIKNITPADRSSSGQPEI
                     DVVAGKPGYERTEDAGTKILRLLRENKGSLPYHDKSDPESIYQFFGMSKKTFKMTTGR
                     LYKEKKILITDTGIQLTP"
     gene            3732..4253
                     /locus_tag="VGQ51_00755"
     CDS             3732..4253
                     /locus_tag="VGQ51_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035108.1"
                     /translation="MGKIVLAGWIVIALVAAGCRNGAEKDVPGGKEYQNETQSKQRIR
                     LPDSSTVILAPGAKIQLGNGFDSGIKELSLDGEAWFDIIRGRVTLHTRDMVVEVLWAG
                     RFHAEAFRARPGEEIDLLEGRIRAKKSYHSDTDNEEEELGPGEMVMINRDIDLMEKEK
                     LNAAELEKLKGAW"
     gene            complement(4259..4645)
                     /locus_tag="VGQ51_00760"
     CDS             complement(4259..4645)
                     /locus_tag="VGQ51_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9035109.1"
                     /translation="MESLSPNLFVRDIRATLAFYQILGFTLTMSVPGEDEGQPIWAMV
                     TNGKVTFMFQTFESLGDKLPRISRTDGGSLLFYINVMHIRDFFDNIREKVPVLHGLEK
                     TFYGATEFSIQDNNNYVLTFAEHEGS"
     gene            4734..5513
                     /locus_tag="VGQ51_00765"
     CDS             4734..5513
                     /locus_tag="VGQ51_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HEV9035110.1"
                     /translation="MHVVSYQIADSIDVKAFRQSYKEGIYHYDADELFYKTGPEDFVY
                     VFKYGVVAFMNYDEQGRDGFLQRIAPFCKNVFGLPLSEEFEVETNAKENKIGYNKIEI
                     IKPDIEVFRLIMLNVSQSVSLDYYSEQTNLLLEETNSHTLTLERKGRLDISGANLKKY
                     IGKTLNLKNRIAENLYIFDSPPETWEDEDLNRIDLGLKRTFDLQERYRDIREGLEMIR
                     ENLELFKDLLQYRNSTVLEWIIIILVALEVLNLVIDKLFHK"
BASE COUNT         1307 a         1193 c         1737 g         1331 t
ORIGIN      
        1 gtttcatttt cgatgaaaaa tcttcataca acggcggcgt gtcggcgccc atataccagg
       61 ccctgcgcaa ggaagccgtc ggtctgaagc gggtggttcc ggtgatgggg ctctatctca
      121 gggccgtacg gagtaatccc gcagatggaa agccggtgac ggtcgaggac caggatggaa
      181 ttgcatctac ggatgccgta tatttttcaa tggtgcctta tcgctggctg gcggggcagg
      241 gcggtactgc gctgaatgca ccgaacaaag tggtgcttac ccggagcagg gcccaacaat
      301 actttccagg caaaacgccg caggagatac tgaacagcac cctgacctat tacagctatc
      361 gcgatacgat cacgaggacg gttgcgggta tcgtcgccga cccggaaggg cccacggagt
      421 ttacgacaaa ggaattctgc tcgctgcccg aaaaagaata ttcgctgaat agctggacca
      481 ataccaacgg cggcgacaag ctgtatcttc aattggagga taacgccgtt ccggcccggg
      541 tgctggcgac catcgaacgc gtggttggtg aaaaggttcg ccagttcgag cagacaagga
      601 aagacaattt taaattcaaa agatggttcg agctgctgcc tatgagcgag tcccatttct
      661 ccacctatat caatgaatac accgtgcaca aagccaataa gaatgtgttg tatgggttga
      721 tgggagtggg cctcttctta ctggtgctgg cttgcatcaa ctatatcaac atgggggtgg
      781 cgtccatacc tcaacgcgcc aaagaaatag gcgttaggaa gacactcggc ggccgccggt
      841 ctgccctgat cgcgcagttt ctgactgaaa cgctgctgac cacctttctc gcatgcctgc
      901 tggcgttcgc attaggatgg ctggaattcc ggctgctcag ggatatcctt ccggataaac
      961 tcgaaccgtg gggtgggatg gggcaggtgg ccgtattcat gggtgttttg gttatcctgg
     1021 taactttgtt atcgggtctt tacccgggat ggctgatcac caaagtaaag accgtggagg
     1081 tgttccggcg cgtcttttta gtaaagaaca ggggtggacg gatcggtctg cagaaagcgc
     1141 tgatcgtttt tcagtttgtg atcgcactgg tgttcatcac gggtgcgatg atcgtaggga
     1201 gtcagctgca ttatgtcatc gactccgata tggggttcaa taaggatgcc gtggtgctcg
     1261 tggatgttcc ctggaaatac agcggcagtc ccctgtataa agacaagcag tacagtctgc
     1321 tggcggaact gaagacgctg cccgggatca ggggtatcgc gttgggtgat caacccctgg
     1381 aaagcggata cagttcgagc gggttcaatt attaccatga tggaaaggcg ccggtatcca
     1441 ggcaggtttt caggaaggag atcgataccg cctatctggg tttttacggc tttcatcttt
     1501 tggcaggcag gaacctgagt ccatcggata ccaccaatga actcgtcatc aacgagactg
     1561 ccgtcaaagc cttcgggttt gcttcgccgc aagacgcatt gggtaagttt atcggtcaga
     1621 atgcgacggg tctcctgccg atcgtcggag tgattaaaga cttccatacg cgggatttct
     1681 ataatacgat cgacccgatg gcttttatgt ccgatcgggg tgtctccacc ttcaacataa
     1741 gactggacaa tgcacatgcg gcggactggc agcagacact gaaggccatc gagaagaaat
     1801 ggtatggctt ttatccgccc ggtagctggt cctataaatt ctatgacgaa acgatcgcag
     1861 aactatataa gaacgagcgg catctttcca cgctgatcga cctgaccacc ggcatctcca
     1921 tattcatcag ctgtctgggt ttgttcggtc tggccgtgct gacggcttat cagcggacca
     1981 aggagatcgg catccgcaag gtgctgggtg caacggtgcc gggcatcgtc ggattgttgt
     2041 ccagggaata ccttcggctg gtggcgctgg cgatcatcat tgccattccc atttcatggt
     2101 gggcggcaga caagtggctg cagaatttcg catataagat accgttgagc tggtggcttt
     2161 ttgctgcggc gggggtatcg ggtctggcga tcgccctggt gactgtgggg ttccgcgcgt
     2221 tgaaagcggc gcgggtgaat ccggtgaatt cgctcaggat cgagtgatga ctatgtcaaa
     2281 tcaaaatata ttgagtgtct taaaggaacg tttcgagcga aacatgcctc gtcacgaggg
     2341 gatccagtgg gcgaaagttg ctgcccggct ggagagcaat cccgggaagc ttcgttcgct
     2401 gaacgaaatg gaaaggaccg gcggggagcc ggatgtcgtc gggttcgatg cacacagcgg
     2461 ggaatacatt ttttatgatt gttcggcgga gagccccaag ggccgccgaa gtctgtgtta
     2521 tgacgatgcg gcgctggaat cgaggaaaga aaataagcct gatggcagcg ctatgggtat
     2581 ggcggcggct atggggatcg ggatattgac tgaagaacag taccggggat tacagaggct
     2641 ggggaagttg gatttgaaaa cgtcgagctg ggtgttgacg ccggcggcta tacgaaagct
     2701 ggggggcgca gtattttgtg atcgccgata tgatacggtt ttcctttatc ataatggggc
     2761 ggaatcttat tatgccgcaa gggggttcag ggggtgggtg agagtataat gcctggtcag
     2821 ggggtgagct ggatgccggt gtcggtgatg aggatctttt tttctttata gaggcggccg
     2881 gtggtcatct tgaaggtttt tttgctcatg ccgaagaact gatagatgga ttcggggtcg
     2941 gatttgtcat gatagggaag gctgcctttg ttttcgcgga ggaggcggag gatctttgtg
     3001 ccggcgtctt ctgtacgttc gtatccgggt ttgccggcga cgacgtcgat ctctggctgg
     3061 ccggaggacg agcggtcggc gggtgttatg tttttaatga agccggggaa gcggtcgccg
     3121 atagcgatat tgctgaagac ctcattgaaa tgcagaacgc cggtatgacg gttgtttatg
     3181 atgacgacat atccgatatc gctacgtcgc caaatcgtga ggtcgacgag gtctttctct
     3241 tttgcggtaa tgtcatcatt gctgagaaaa ggctcgatct tttcggtggc ggccagacgg
     3301 cccgtctttt cgtcgagata gatctttacg agatagtcgc cgccgaatcg cataccgcta
     3361 agctgtttgg atttggggac gaagaggtct ttcatcaacc cccaatcgag gaaggcgccc
     3421 agcggagtga cgctgaccac gcggagtaat gcgatatcac ctaaaatggc tttggctcgt
     3481 tgggtagtgg cgatgaggcg gtcttctccg tcatgataga ggaagacgtt gagttcgtcg
     3541 ccgatgcggg cgcctcgcgg gacgaaacgt ttgggaagaa ggatgccgcc ttctccgtca
     3601 tcgaggatca gaccgaattc cagttccttt tttactttta gagtgttgta ttctccgaca
     3661 tggaccatgg ggcaaaggta ggtcaaaaac aggagccgca tttttcgcag atattcgtag
     3721 attcatggcc tatgggaaag atagtcttgg ccgggtggat cgtcatcgcg ttggttgcgg
     3781 ctggttgccg gaatggggca gagaaagatg tgccgggcgg aaaggaatac caaaatgaga
     3841 cgcaatcaaa gcagcggatc aggctgcccg atagtagtac cgtgatcctc gcgccgggcg
     3901 caaagatcca actgggcaac gggttcgaca gcggaattaa agaactgtcg ctcgatggag
     3961 aagcgtggtt cgatatcatt cgcgggcggg tgacgctgca tacgcgggat atggttgtcg
     4021 aagtcctgtg ggccggcagg ttccatgccg aggcattccg ggcgcgaccg ggggaggaga
     4081 tcgatctgct ggaaggacgt attcgggcga agaaatccta ccattcggat acggataacg
     4141 aggaggagga actggggccg ggagaaatgg tgatgatcaa tagggatata gatttgatgg
     4201 aaaaggagaa gttgaatgca gcggaattgg agaagttgaa gggggcgtgg tagggcgatc
     4261 aggatccttc gtgctcggcg aaggtcagca cataattatt gttatcctgg atcgagaatt
     4321 cggtggcgcc atagaaggtc ttttcgaggc cgtgaaggac ggggactttt tccctgatgt
     4381 tatcgaagaa atcgcggatg tgcattacgt taatatagaa gaggagggag ccgccgtcgg
     4441 tgcggctgat gcggggcagc ttatcgccga ggctttcgaa ggtttggaac atgaaggtga
     4501 ccttgccgtt ggtgaccatt gcccagatgg gttgaccttc gtcctcgccg gggacggaca
     4561 tcgtgagcgt gaatccgagg atctggtaaa aggcgagtgt cgcgcggatg tcgcggacga
     4621 agaggttggg ggagagggat tccatatact tatttttgca gcaatatact aaatagttta
     4681 gtatatcgaa caaatattgc cacccattta ttttgttatt cggagggatt tgtatgcacg
     4741 ttgtttccta ccagattgcg gacagcatcg acgtgaaggc ctttcggcag agctataaag
     4801 aagggatcta tcactatgat gcggacgaat tgttctacaa gacgggaccg gaggattttg
     4861 tgtatgtatt caagtatggg gtcgtcgctt tcatgaatta cgatgaacag ggaagggacg
     4921 gatttttaca gcggatagcc cccttttgca aaaatgtatt cggtctgccg ctgagcgagg
     4981 aattcgaggt ggagaccaat gcgaaggaaa acaagatcgg ctacaacaag atcgagatca
     5041 tcaaaccgga catcgaggtg tttcggctga tcatgctgaa tgtcagccag tcggtgtcgc
     5101 tggattatta ttcggaacaa accaatctgc tgctggagga gaccaactcg cacacactca
     5161 cactggaacg gaaggggcgg ctggatattt cgggcgccaa tctaaagaaa tacatcggga
     5221 agacgctgaa tctgaagaac cgtatcgcgg agaatctgta catcttcgac agcccgccgg
     5281 agacgtggga ggatgaggac ctgaaccgga tcgatcttgg attgaagcgt acgttcgatc
     5341 tgcaggaaag atatcgggat atccgggaag gcctggagat gatcagggaa aatctggaac
     5401 tgttcaagga tctgttgcag taccggaata gtacggtgtt ggaatggatc attatcatcc
     5461 tggtggcgct ggaggtgctg aacctggtga tcgataaatt gttccataag tgattgaaaa
     5521 gggttatatt tcaagaatga tcatatgtat gtatgatgtt gattaata
//
LOCUS       DASYSV010000028        12350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1159897, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000028 DASYSV010000000
VERSION     DASYSV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12350
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159897"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3090
                     /locus_tag="VGQ51_00770"
     CDS             <1..3090
                     /locus_tag="VGQ51_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9035111.1"
                     /translation="NKEGGTMTAELTRTGVDDRNIVLTGRIVDEGKGTPLEGVSISIK
                     GTNKGTLTDKDGNFRLDVPDKDAVLVISLIGYQTREETVGSRTDLQLTMVSQSQDLND
                     VVVVGYGTQKKSVVTGSISSVKSSDLDDQPVYRLEDALQGRASGLTIAASSGQPGSSS
                     TVRVRGTTSINGSDPLYVVDGVPLDGGGLDYLNAADIESIEVLKDAATAAIYGARAAA
                     GVILVTTKKGRAGQMRVSYDGYYGTQEPARKLQLLNATQYATLRNESSLAGGKGIVFA
                     NPASLGNGTDWQSQIFDNNASIMSHELSFSGGTDKSTFYTSLGYFDQNGIVAAPISWY
                     KRLTARFNGAHKLKPWLNFGENIGYSYIRNQGLGNTNSEFGGPLSSAINLDPITPVVI
                     TDPNILSQPPYSNEPVMTDAQGHPYAISNYVGQEETNPLAYIKIHQGNGYNWSHNFIG
                     DAYVSVTPVRGLELKSTIGTKLAFWGSNSFNPIYYLNGSSSNPITNYYRDAENLFIWD
                     WENTASYNRHIGLNNVTAMIGTGAWEQTASGLNGNFQYMPVTSLGQATPNYSLPNADK
                     VANGYDNVAHTVSSEFGRLSYSYDDKYLLMAQIRRDGSSRFGSNYKYGYFPGASIGWV
                     VTRENFFPANPVVTFLKIRGSYGVTGSDNIGDFLFESTVSGGRNYTIGNTYEIGYSNN
                     APPNPNLKWEQTAQTDVGFDAILMSHLSVGFDIYNKKTTGMLLAEQLPMYVGASGQPT
                     GNIADMTNKGIELELGYTKKIGQVNLSMKANGSLLQNRVTSLGVTPYRTGATLQSSAY
                     ELSRTTPGHAIGSFYGFQSLGIFQTQADVNGYVGKSGAPVQPNAKPGDFKWADLDGDG
                     KITAGDRTFIGDPTPKYAVGFTVSASWKNFDVLVFGQGAGGNKIFQGLRRLDIQTANY
                     STKALSRWTGPGTSNDFPRLVDGDPNSNFTNPSSFYLEDGSYFRIKNLQIGYTLPQGL
                     LRQWTIQKVRIYVSGYNLITFTKYTGYDPEIGGSSYGIDRGFYPQACSFQAGLSVGF"
     gene            3113..4621
                     /locus_tag="VGQ51_00775"
     CDS             3113..4621
                     /locus_tag="VGQ51_00775"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9035112.1"
                     /translation="MNRTTIIYFAFVTGVTIGATGCKKSFLDVKPQGTQLESDYYQTP
                     DEAFAGLVAAYNPLSWTVVSSYAPKMVLLSLASDEAYAGGGSNSDNPGMQAWNTYTLA
                     AATPNVPADLWNRNFTGIARVNTMLSKIGNVPGLTTDLKTRYTAEMYFLRAYYYFDLV
                     RLFGNVPLITTVLSQSEWFTQVQAKPADVYAQIEKDLNAAIPGLPATVNPSTEGGRVT
                     KGAAQALLGKAIIYENNNSRMQEAANDLDQVNTSPNYHLLPNYADIFNPGNKFNAESI
                     FEIVHTAAAGKGWGNWPNFDGNVGVQMVGPRGYSGPTYEAATAGYGFCPITMNLVNAL
                     KNDPRYAATIVNIDSLSQAGVCKYQPGYQNTGYFINKFAPLAAFQGTVGDIPLNWTND
                     EIEIRLADTYLLEAEALVRGGGSAAKAQSLLDAVRARVGLPSVPATLDNIYNERRLEL
                     ATEGHRFFDLVRTGRAASVLGFKGFTAGKNEVLPIPLQELNNTKLVQNPGYN"
     gene            4646..5497
                     /locus_tag="VGQ51_00780"
     CDS             4646..5497
                     /locus_tag="VGQ51_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035113.1"
                     /translation="MRNLFFCCGLGLALAAICVSGCTPDNKSASLSALPKASFTVTPL
                     SGATNTYICSAGTQGVFAWYWSSGNGNPSPGTSSDTIHFSQHGNYRIVLTAVGEGGYD
                     TTSQVVSVANDDLGNTLIAGGMLNTASSSSWTFLNTGGTATTFTFGANGLVLSNTGSG
                     ANTNGGVYQAIQVQAGVNYKFSAIVQGAGINASWVEFYIGATVPTQGSDYSDNKIYSL
                     NWWAGCAHNAFNGDVVSIGCSGSGPSSGTITFPTSGTYYVVIKAGSAGGNLGSGGVTV
                     TDILLDH"
     gene            5510..6694
                     /locus_tag="VGQ51_00785"
     CDS             5510..6694
                     /locus_tag="VGQ51_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEV9035114.1"
                     /translation="MARPLIFLLVVPVMGCSKGDHGGGQPNTGSQPSVKVTDVTQARQ
                     TQASAFRFYIDLSAAGSQAVSVSYATSDGTAKAGVDYTAESGTITIPAGQTEVYVDVP
                     VSGDSLRRANQTFSFALGTVTNAVVGTARAMGTILNTDLLYLPTDSTGYTTPASYPGR
                     TLVWSDEFNGNALNTQDWNYEQGGSGWGNNELENYTNRTQNVFVSAGHLVIEARQESY
                     GGNNYTSGRLTTQGKRSFTYGRIDIRAKLPVSKGMWPALWMLGSDISTVPWPGCGETD
                     IMELIGTYPGRVTGSLHWQQSGGSEGTYNNNYNLNTGDFSQQFHVYSIIWQKDSVQFL
                     VDDQPYVNGGASDVTSGTYPFNSPFFFIFNVAVGGNWPGPPDGTTVFPQRMFVDYVRV
                     FQ"
     gene            6699..8153
                     /locus_tag="VGQ51_00790"
     CDS             6699..8153
                     /locus_tag="VGQ51_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HEV9035115.1"
                     /translation="MKTIMIFLMGVVVVGMCSCNKKSNPAPVTPPPDTTVTPPPAGSQ
                     VALWLTNTDKTALFRLQNVTLDFGTGLAVEPVITVDTTQTYQTIDGFGYALTGGSAQL
                     IYSLPTAQRQALEQELFATDSTNIGVSYLRLTIGASDMSARVFSYDDNASPSAPDTTL
                     ANFDLSDDKTYLVPLLKEILAVNPSIKLLGCPWSPPAWMKSNNSASGGSLLPQYYAVY
                     AQYFVKYIQAMKAAGITIDAVTPQNEPLNANNNPSNVMQDTAENIFVRDYLGPAFHTA
                     GIATKIIVWDHNCDVPSYPEYIFSDPVAAQFVDGSAWHLYGGSISALSTVHDAFPSKN
                     IYFTEQYVGGPGNFQGDLDWAVQNLIVGATRNWSRNVLEWNMAADASYGPHTVGGCGN
                     CQGAFTINGTSVLRNTSYYIIAHASKFVRPGSVRVASNIAGTLQNVAFVVPGGKKVLI
                     VLNTGSGAQTFTVQFNGKNFSTTLGGYAVGTYVW"
     gene            complement(8169..9848)
                     /locus_tag="VGQ51_00795"
     CDS             complement(8169..9848)
                     /locus_tag="VGQ51_00795"
                     /inference="COORDINATES: protein motif:HMM:NF036995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HEV9035116.1"
                     /translation="MKSILSPKYQALVQDLKEKIRRARIRAISAVNKEMLLIYWEIGK
                     VISELEQEEGWGAGIVDKMAQDLKIEFPDFKGMSPRSLRYMREFYISYPLLSPPATRS
                     KSLQQRAAKPSTPKGGESYRILQPPAAKLTVQELLQQVVNQIPWTYHQVLLDKVKDNE
                     ERLFYMQKAILNGWSRNILALQIDNRLHEREGKSITNFNERLPEPQSDLANATLKSPY
                     VFDFLSFEDEIQERELEKALIHHLEKFMLELGRGFSFVGSQYRINIDGNEYFPDLVFY
                     NFLLQCFVIFELKVGEFKPEFAGKLNFYVTAFDNRIKNEKHNRTIGVLLCKTPSETVI
                     RFSLEKIDSPIGVSDYNLAKSIPKELKSGMPTIKELEHELIQEIERLKGPAEKRWDNL
                     KKKLATLNKGEIKKTANQEILNALFDGSLKPLLDGLAERLKEFGPEFISSTYGWSANN
                     TGNPSLGGLEAGWKQGSILKDSREIQFWYRLNGLKRAGVEGTDVYVTLVYTMDQYWYG
                     FKMFNYNNQQPFIKKLYDEQLTAQDISQIIDLVCEQIMDSIESIIDRMNGH"
     gene            10260..11432
                     /locus_tag="VGQ51_00800"
     CDS             10260..11432
                     /locus_tag="VGQ51_00800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV9035117.1"
                     /translation="MTKVVLKKKISRRVESGHPWVFANEIAGVEGDTAGGSIVDVYTH
                     DKKFLGRGYINLQSQIAVRLLTRDRNETIDDNFFYGRLQEAWGYRQRIGYTENCRLVF
                     GEADYLPALIIDKFNDYFVLQTLSLGMDRWKPAIVAALQRIFQPKGIYERNDVPVREL
                     EGLAQLKGYLSEPFDTQIVIRENGLRLHVDIENGQKTGYFLDQHDNRRAIQNIVRDAD
                     VLGAFCYTGSFETHAGYYGARSVLGLDISENAVAQARRNAELNGLTDVCRYEAVNAFD
                     ALKQWSKEGRQYDVVMLDPPAFTKSRETIQKAITGYKEINLRGMKMVKKGGFLVTSSC
                     TNLVQPELFLETIQLAAKDARRRLRQVTFQAQASDHPIVQGWENTNYLKFLIVQVL"
     gene            complement(11434..11739)
                     /locus_tag="VGQ51_00805"
     CDS             complement(11434..11739)
                     /locus_tag="VGQ51_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9035118.1"
                     /translation="MANQADCQVRGPMPPEPEHKVIKFYPNPAVSYITFELARDANNK
                     TFTLQIFSFLGRMVKNVPDISDKTTVQLSDLTRGLYTFQLKDETGRTTDSGIFQIIK"
     gene            complement(11906..>12350)
                     /locus_tag="VGQ51_00810"
     CDS             complement(11906..>12350)
                     /locus_tag="VGQ51_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEV9035119.1"
                     /translation="PDFTLYDSDKQKVNLSDLKGRNVLLLFFPQAFTGTCTKELCSTR
                     DNISLYNEANAQVLGISVDSVFTLARYKEDQRLNFPLLSDFNKETSTAYGSLYDSFVF
                     DMKGVSKRSAFVIDKNGIIRYAEVLEKATDLPDFAAIQKTLQELN"
BASE COUNT         2882 a         2802 c         3691 g         2975 t
ORIGIN      
        1 aacaaagaag gcggcactat gacggccgaa ctgacgagga cgggtgtcga cgaccggaat
       61 atcgtattga cgggtcgtat cgtagacgag gggaagggta cgccgttgga aggggtcagc
      121 atatcgatca aggggacaaa taaaggaacg cttaccgaca aagatggcaa cttcaggctg
      181 gatgtcccgg ataaggatgc tgtcctggtg atctcgctga tcgggtacca gaccagggag
      241 gagacggtcg ggagccggac ggatctgcaa ctgaccatgg tttcgcagtc gcaggatctg
      301 aacgacgtcg tcgttgtcgg ttatgggaca cagaaaaaga gtgtggtcac ggggtcgatc
      361 tcgagtgtaa aatccagcga tctggatgac cagccggtct accggctgga agacgcgttg
      421 cagggaaggg catcgggcct gacgatcgcc gcctcgtcgg gtcagcccgg ttcttcctcc
      481 accgtgaggg taaggggaac cacttcgatc aacggaagtg atccgttgta tgtggtagac
      541 ggtgttccgt tggatggagg cggacttgat tatctgaatg ccgccgatat cgaatccatt
      601 gaagtcctga aggatgctgc taccgccgcg atctatgggg cgcgggcggc cgcgggcgtt
      661 atcctggtga ccacgaagaa gggcagggcg ggacaaatga gggtaagtta cgatggatat
      721 tacggcacgc aggagccggc aagaaagctg caattgctga atgcgacgca gtatgccacc
      781 ttgcgcaacg aatcttcgct ggcgggtgga aaagggatcg tttttgccaa tcctgcatcg
      841 ttgggtaacg ggacggactg gcaaagccag atcttcgata acaatgcgag catcatgagc
      901 catgagctct ccttcagcgg ggggacggat aaatcgacct tttatacttc gttggggtat
      961 ttcgatcaaa acggcatcgt agcggctccg atatcctggt ataaaaggct gacggcccgt
     1021 ttcaacggag cgcataagct aaaaccctgg ttgaatttcg gggagaatat cggctattcc
     1081 tatatcagga atcaggggtt gggcaatacg aacagcgagt tcggcggtcc cctgagctct
     1141 gcgatcaatc tcgaccctat tacgcccgtg gtcattaccg atccgaatat actcagtcaa
     1201 cctccctaca gcaacgaacc cgtgatgacc gatgcgcagg gtcatcccta tgccatctcc
     1261 aattatgtcg gacaggaaga gaccaacccg ctggcctata tcaagatcca tcagggcaac
     1321 ggctacaact ggtcgcataa ttttatcggg gatgcttatg tgtcggtgac tcccgtgcgc
     1381 gggctcgagc tgaagagcac catcggaacg aagctggcct tttggggcag caacagcttc
     1441 aacccgatat attatctcaa tggctcgtcc tccaacccga taacaaatta ttaccgggat
     1501 gccgagaacc tgttcatctg ggactgggag aatacggcct cctataaccg tcatatcgga
     1561 ttgaacaatg tcaccgcgat gatcgggacg ggggcctggg agcagactgc gtcggggcta
     1621 aatggcaact tccagtatat gccggtaacc agcctgggtc aggccacccc caactacagt
     1681 cttccaaatg ccgataaggt tgccaacgga tatgacaatg tcgcgcatac tgtaagctcc
     1741 gagttcgggc ggttgagcta cagctacgat gataaatatc tcctgatggc gcagatcagg
     1801 agagacggta gttcaaggtt cggctccaac tacaaatacg ggtatttccc gggagcatcc
     1861 ataggctggg tcgtcacgag ggagaatttc ttcccggcaa acccggtcgt cactttcctg
     1921 aagatccgag gatcatatgg ggtgacaggg agcgacaata tcggggattt tcttttcgag
     1981 tccacggtca gcggtggccg caactatact atcggcaaca cttatgagat agggtatagc
     2041 aacaatgcgc cacccaatcc caatctgaaa tgggaacaaa cggcacagac cgacgtgggt
     2101 ttcgacgcca tactgatgag tcatctttcg gtgggtttcg atatctacaa taagaaaacg
     2161 accggcatgt tgcttgccga gcagctgcca atgtatgtcg gcgccagcgg tcagcctacc
     2221 gggaacatcg ccgatatgac caacaagggc atcgagctgg aactggggta taccaaaaag
     2281 atcgggcagg tgaacctcag catgaaagcc aacgggtcat tattgcagaa ccgggtgacc
     2341 tcgctgggag tcacaccata ccggaccggg gctacgcttc aatcgagcgc ttatgagctg
     2401 agccgcacca cgccgggaca tgccatcgga tcgttctatg gcttccaatc gctgggcatc
     2461 ttccagacgc aggcggatgt caatggatat gtgggtaaat ccggagcgcc ggtacagccc
     2521 aatgcaaagc ccggtgactt caagtgggcc gatctggatg gggatgggaa gatcacggcg
     2581 ggtgaccgga cgtttatcgg cgaccctacg ccgaaatatg ctgttggttt tactgtcagc
     2641 gcctcgtgga aaaatttcga tgttcttgtc ttcgggcagg gagcgggcgg caacaagatc
     2701 ttccagggtc tgcgccggtt ggatatacag acggccaact attcgacaaa agcgcttagc
     2761 cgctggacgg ggcccggaac atccaatgac tttccacggt tggtggatgg ggatcccaat
     2821 tccaatttta ccaacccttc ctcattttac ctggaagatg gcagttattt ccggatcaag
     2881 aacctgcaga tcggctatac gttgccgcag ggcctgttgc gtcagtggac gatacagaaa
     2941 gtgcggatct atgtcagcgg gtataacctg atcactttta cgaaatatac cgggtatgat
     3001 ccggagatcg gcggcagcag ctatgggatc gacaggggct tttatccgca ggcctgttcg
     3061 ttccaggctg gactcagtgt cggattctaa cgattataaa aaaatgaaaa tcatgaacag
     3121 gacaaccata atatattttg cgtttgtgac gggggtcacg ataggagcga ctggctgcaa
     3181 aaagagtttt ctcgatgtga aaccacaggg gacgcagctg gaaagcgatt attatcagac
     3241 gccggacgaa gcgtttgccg gattggtagc ggcttacaac ccgttgtcat ggaccgtggt
     3301 cagctcgtat gctcccaaga tggtgctgct gagcctggcg tcggatgaag cctatgcggg
     3361 cggcggatcg aactcggaca accccggcat gcaggcctgg aatacgtata ccctggcagc
     3421 ggccaccccg aatgtgcctg ccgacctgtg gaaccggaat tttacgggga tcgcgcgtgt
     3481 caacactatg ttgtcgaaga tcggcaacgt tcccggtctg accaccgatc tgaagacaag
     3541 gtacacggcg gagatgtatt tcctgcgggc ctattattat ttcgatctcg tgcggctgtt
     3601 cgggaatgtc ccgctgataa ccacagtgct tagccagagc gaatggttta cgcaagtgca
     3661 ggcgaagcct gccgacgtgt atgcccagat cgaaaaggat ctgaatgcgg ccattcccgg
     3721 gttacccgcc accgtgaatc cttcgacgga aggcgggcgt gtcacgaagg gggcagcgca
     3781 ggcattgtta gggaaggcca ttatttacga gaacaataat tccaggatgc aggaagccgc
     3841 gaacgacctg gaccaggtga atacttcccc caactaccat ctgctgccaa actatgcgga
     3901 tattttcaat cccggcaaca agttcaatgc ggaatcgatc ttcgagatcg tgcatacggc
     3961 ggcggccggc aaaggttggg gcaactggcc caatttcgac ggtaatgtag gcgtgcagat
     4021 ggtgggcccc cgcggatatt cgggaccgac ctatgaggct gcaaccgcgg gatatggttt
     4081 ctgtccgata acgatgaacc tggtgaatgc cctgaagaac gatccccggt atgcggctac
     4141 gatcgtaaat atcgacagtc tttcgcaggc gggggtttgc aagtaccagc caggttatca
     4201 gaacacgggt tattttatca acaaattcgc gccactggct gcatttcagg ggactgtggg
     4261 ggatatcccg ttgaactgga cgaatgatga gatcgagatc cggctggccg atacttatct
     4321 gctggaagca gaagccctgg tgaggggtgg tggttctgcg gcaaaggcgc agtcgctgct
     4381 cgatgccgtc cgggcacggg tagggttacc gtcggtgccg gccacactgg acaatatcta
     4441 caacgagcgg agactggagc tggctaccga agggcatcgc ttcttcgacc tggtgcgtac
     4501 gggacgggcg gcgtcggtgc tgggtttcaa ggggttcacg gcgggtaaga atgaagtgct
     4561 gccgataccc ctgcaggaat tgaataacac gaaacttgtt caaaatccgg gatataatta
     4621 ataggatctt aaaaattatt gccatatgcg aaatctcttt ttttgttgcg gactggggtt
     4681 ggcgctggct gcgatctgtg taagcggctg tacgcccgat aacaaatcgg cctcgctgag
     4741 tgcattgccc aaagccagct ttacggttac cccattgagc ggggctacga atacctatat
     4801 ctgttcggcc ggtacgcagg gtgtctttgc ctggtactgg agctcgggga acgggaatcc
     4861 ctctccgggt acttcgagcg atacgatcca tttctcgcag cacgggaatt acaggatagt
     4921 gctgaccgcc gtgggtgaag gcggatacga tacgaccagc caggtagtat ccgtagcgaa
     4981 tgacgatctg ggaaatacgt tgatcgccgg tggcatgttg aatacggcga gctcgtcttc
     5041 ctggaccttt ctgaatacgg ggggaaccgc gacaactttt acctttggcg cgaacgggct
     5101 ggttttgagc aataccggtt caggcgccaa tacgaacggg ggcgtttatc aggccatcca
     5161 ggtgcaggcc ggggtcaatt ataaattttc cgcgatcgtg cagggtgcgg ggatcaatgc
     5221 atcctgggtg gagttctata tcggcgccac cgtgccaacg cagggatcgg attattcgga
     5281 taataagata tattcgctga actggtgggc gggttgtgcc cataatgcct ttaatgggga
     5341 tgtggtcagt atcggttgtt caggatcggg tccgtccagc gggactatca ccttcccgac
     5401 cagtggtacg tattatgtcg tcatcaaagc gggcagtgcg ggtggtaatt tagggtcggg
     5461 cggcgtgacc gtcacggata tcctgttgga tcattgataa atttgttcga tggcaagacc
     5521 cttgatcttt ttgttggtgg tgccggtgat gggctgctcg aagggcgacc atgggggcgg
     5581 gcaaccgaac acgggcagtc agccgtccgt gaaggtcact gatgtgacgc aggcgcgtca
     5641 gacgcaggca tcggcattcc ggttctatat cgatctcagc gcagccggtt cccaagccgt
     5701 tagcgtgtct tatgcgacct cggacgggac ggcaaaggcg ggggtcgact atactgccga
     5761 atcgggaacg attacgatcc ctgcggggca gacggaggtt tatgtcgacg tgccggtgtc
     5821 gggggacagt ctccggcggg ccaatcagac cttttctttt gctcttggta ccgtcacgaa
     5881 tgcggttgtg gggacggcca gggccatggg gacgatcctt aacacggacc tattgtatct
     5941 gcctaccgat agcacgggtt atacgacgcc agcgagctat cccgggcgta cgttggtgtg
     6001 gagcgacgag ttcaatggga atgcgttgaa cacgcaggac tggaattatg aacagggcgg
     6061 atcggggtgg ggcaacaatg agctggagaa ttacacgaac cggacgcaga atgtttttgt
     6121 ttcggcgggg cacctggtca tcgaggcaag acaggagtcg tatggcggca acaattatac
     6181 ttcggggcgg ctcacgacgc aggggaaacg gtccttcacg tatggccgga tcgatatccg
     6241 cgccaaattg ccggtatcga aaggcatgtg gccggccttg tggatgctgg gctcggatat
     6301 ttctaccgtg ccctggccgg gatgcggaga aacggatatc atggaactga tcggcactta
     6361 tccgggtcgc gtgacgggtt cattgcactg gcagcaatcc ggagggagcg agggcactta
     6421 caataacaat tacaatctta atacgggaga cttttcacag cagtttcatg tatatagcat
     6481 tatctggcag aaggacagcg ttcagttcct ggtggacgat cagccgtatg tgaacggagg
     6541 ggcgtcggat gtgaccagcg gaacctatcc ctttaatagc ccattctttt ttatctttaa
     6601 tgtagcggtc ggagggaact ggccggggcc gccggatggg acgacggtct tcccgcagcg
     6661 gatgtttgta gattatgtgc gggtgttcca gtaataatat gaaaactata atgatttttt
     6721 tgatgggggt ggtggtggtc gggatgtgct cctgcaacaa gaagagcaat ccggctccgg
     6781 tgactcctcc tcccgacacg acggtgactc ctccgccggc gggctcgcag gtagccttgt
     6841 ggctgacgaa taccgacaag accgccttgt tccggctgca aaacgtcact ttggatttcg
     6901 ggaccggtct ggccgtcgaa ccggtgatca ccgtggatac gacgcagacc tatcagacca
     6961 tcgacgggtt cggttatgcc ctgacggggg gcagcgcgca gctgatctat agtcttccga
     7021 ccgcgcagcg gcaagcgttg gagcaggagc tcttcgccac ggacagtacg aatatcggag
     7081 tgagctattt gcgtctgacc atcggcgcat cggatatgag cgcgcgggtc ttttcgtatg
     7141 acgacaatgc ttcgccgtct gcgcccgata cgacgcttgc caacttcgat ctgtcggatg
     7201 acaagaccta tctggtgccg ttgctgaaag agatattggc ggtgaatcct tccatcaaac
     7261 tgctgggttg tccgtggtcg ccgccggcct ggatgaagag taacaatagt gcgtccgggg
     7321 gatcgttgct gccgcagtat tatgcggtat acgcgcaata tttcgtgaaa tacatccagg
     7381 cgatgaaggc ggcagggatc acgatcgatg ccgtcacgcc acagaatgag ccgttgaatg
     7441 cgaataacaa tcctagcaat gttatgcagg atactgccga gaatattttt gtgcgggatt
     7501 atctggggcc ggcttttcat acggctggta tcgccacaaa gatcattgtc tgggatcata
     7561 actgcgacgt gccgtcctat cctgaatata ttttcagtga cccggtggcg gcgcagtttg
     7621 tggatggctc tgcgtggcat ctttatggag gaagcatctc ggcgttgtct acggttcacg
     7681 atgcttttcc ttctaagaat atctatttca cggagcaata tgtgggaggg ccgggtaatt
     7741 tccagggtga tctggattgg gcggtccaga atctgatcgt cggcgcgacg cgcaactgga
     7801 gccggaatgt attggaatgg aatatggcgg cggatgccag ttatggtccg catactgttg
     7861 gcgggtgcgg aaattgccag ggggcgttca ctatcaatgg gacgagtgtt ttgcggaata
     7921 cttcgtatta tatcatcgct cacgcttcga aattcgtgcg gccgggctcg gtgcgggtgg
     7981 cgtcgaatat cgcgggtacg ttgcagaatg tggcttttgt ggtgccgggg gggaagaagg
     8041 tgttgatcgt gttgaatact gggagtgggg cgcagacgtt tacggtgcag ttcaatggga
     8101 agaatttttc gacgacgttg ggtgggtatg cggtggggac gtatgtgtgg tagcggcgac
     8161 tgtttatttt agtgtccatt catgcgatca atgatgcttt ctatgctatc cattatttgt
     8221 tcgcagacca gatcgattat ttgtgagatg tcctgggcag ttagctgctc atcgtagagt
     8281 ttttttatga aaggttgctg gttattgtaa ttgaacattt tgaagccata ccagtattgg
     8341 tccattgtgt aaacaagagt gacatatacg tcagttcctt caactccagc ccttttaaga
     8401 ccattcaacc tataccaaaa ttggatctcc cggctgtctt taagtatgga cccctgcttc
     8461 caacccgctt cgagcccgcc taaggaggga ttgccagtgt tatttgctga ccatccataa
     8521 gtagatgaaa taaattccgg gccaaactct tttaggcgct ctgcgaggcc gtctaaaaga
     8581 ggctttaaac taccgtcaaa gagggcattg agaatttcct ggttagctgt tttttttatt
     8641 tcgcctttgt ttagggtggc caatttcttt ttcagattgt cccagcgttt ttcagcgggt
     8701 ccttttaatc tctcaatttc ctggattagc tcatgttcca attcctttat cgtcggcatc
     8761 ccgcttttga gttccttggg aatggactta gctaagttgt agtcagatac tccgattggg
     8821 ctatcaattt tttcaaggga aaaacggatg accgtttcgc taggtgtctt acaaagtaat
     8881 acaccgatgg tgcgattatg tttctcattc tttattctgt tatcaaatgc ggtgacgtaa
     8941 aaatttagtt tacctgcaaa ttccggcttg aactcgccca cttttagttc aaatattaca
     9001 aagcattgaa gaagaaaatt ataaaataca aggtctggga aatactcatt gccgtcaata
     9061 tttatccggt attgactgcc cacaaaggaa aatccccgac ccaattccag cataaatttc
     9121 tccaagtggt ggattaacgc tttctctagt tctcgctctt gaatttcatc ttcaaagctg
     9181 agaaaatcga aaacataggg gcttttcagc gtcgcattag ccaagtcgga ctgtggctcg
     9241 gggagcctct cattaaaatt agttatggac ttgccttctc tttcatgaag gcggttgtcg
     9301 atttggagtg cgagtatatt tcggctccat ccgttcaata ttgccttttg catgtaaaat
     9361 agccgctctt cattgtcttt caccttatct aagagtacct gatgataagt ccaggggatc
     9421 tgattgacaa cttgttgcag caattcttgg acagttaatt ttgcagcagg cggctgcaag
     9481 attcgataag actctccacc ttttggagtc gacggttttg cagcacgttg ctgcaaactt
     9541 ttagacctgg tcgctggagg cgataataaa ggataggaaa tataaaactc cctcatatat
     9601 cgcagacttc ggggtgacat ccccttgaag tcagggaatt caattttaag atcctgggcc
     9661 attttatcga cgatccccgc tccccagcct tcttcttgct ccaattccga aataacttta
     9721 cctatttccc aatagataag cagcatctcc ttattcactg ccgaaattgc ccgaattctt
     9781 gcgcgacgaa tcttctcctt taagtcctgg acaagggcct gatatttagg ggacaaaatg
     9841 gacttcataa gtaaatttac acatccttca tggggattta gaaatttact ttgtcatcag
     9901 ggagaaggta aagcatccaa ggtattgcaa tcttctgaac ttgatgttag tttgaaatgc
     9961 ggccaagagg aaggataatg cggaggaatt aagtgaacaa ggccttcggt cgcgcgtcag
    10021 ggatcgaagc ggcagcccgg agccggaact gagcgcagcg aaggtatccg agccaaagcg
    10081 aaggatgcga atggggaccg agcttgcggc tcgggccgaa tttccggcga ggactatagc
    10141 gtagagcccg acccgagtcg cgcggggggg gggtgcgcgg ggcggtgagc gcgggctggt
    10201 gtgcggtggg cgaggggggc gcccgaataa attgcgggcg acgatgtacc tttgcgcgca
    10261 tgacgaaagt agtattgaag aaaaagatca gccggcgggt ggagagcggg catccgtggg
    10321 tatttgcaaa tgagattgcg ggggtggagg gggatacggc cggcgggagc attgtcgacg
    10381 tatatacgca tgataaaaaa tttttgggga ggggctatat caatctgcag agccagatag
    10441 cggtccggtt gctgacgcgc gatcggaacg agaccatcga cgacaacttt ttttacgggc
    10501 gattgcagga ggcctggggt taccggcagc ggatcggtta tacggagaat tgccggctcg
    10561 tgtttggcga ggccgactat ttgccggcgc tgatcatcga taaattcaat gattattttg
    10621 tgttgcagac tttgtcactg gggatggatc ggtggaagcc tgcgatcgtc gcggcgttgc
    10681 aacggatatt tcagccgaag ggtatctatg agcgtaatga cgtgccggtg cgggagctgg
    10741 aggggttggc gcagttgaag ggatatttgt ctgagccttt cgatacgcag attgtcattc
    10801 gggagaatgg gttgcggttg catgtggata tcgagaatgg tcagaagacg ggatattttc
    10861 tggatcagca tgataatcgt cgggcgatac agaatatcgt cagggacgct gatgtgttgg
    10921 gggcgttttg ttatacgggc agttttgaaa cgcatgccgg atattatgga gcgagatcgg
    10981 tgctggggct ggatatttcg gagaatgcgg tagcgcaggc gcggcggaat gccgaattga
    11041 acgggttaac tgatgtgtgc cggtatgagg ccgtgaatgc gttcgatgcc ctgaagcagt
    11101 ggagcaaaga gggcagacaa tatgatgtgg tgatgctgga tccgccggca ttcacgaaaa
    11161 gccgggaaac gatacagaag gcgatcacgg ggtataagga gatcaatttg cgggggatga
    11221 agatggtgaa gaagggaggg tttttggtga cttcttcgtg tacgaacctg gtgcagccgg
    11281 agttgttcct ggagactata cagttggcgg cgaaggatgc aaggagaagg ttacggcagg
    11341 tgacgtttca ggcgcaggcg agtgatcacc cgatcgtgca ggggtgggag aatacgaatt
    11401 atttaaaatt tctgatagtg caggtgctgt aggctacttg atgatctgga agataccgga
    11461 gtcggtggtt cgaccggttt cgtctttgag ctggaaggta tagaggccgc gggtgagatc
    11521 actgagctgt acggtggtct tatcggagat atccgggaca tttttgacca ttcttccgag
    11581 gaagctgaag atctgcagcg tgaatgtttt attattagcg tccctggcga gttcgaaagt
    11641 gatgtaagaa acggcagggt tgggatagaa cttgataacc ttgtgttcgg gctcgggggg
    11701 cataggaccc ctgacctgac aatctgcctg gttagccatc agtagagtaa aggataacaa
    11761 aatgtaaaaa atcctcattg ccctggtctt taacggattg agaccaaatt acttgtttaa
    11821 tcaaagatac gaatttgcgg ggaagaataa aagggtaaaa atacgtttta tcggacggca
    11881 aggactggtg ataacatgct gataatcaat tcaattcttg taatgttttc tggatggccg
    11941 caaaatcggg gaggtcggtg gccttttcga gcacctcggc ataccggata atgccgtttt
    12001 tgtcgatgac gaaggcggag cgtttggaga cgcctttcat atcgaagacg aaggagtcgt
    12061 atagcgagcc ataggcggtc gaggtctctt tattgaaatc gctgaggagg gggaaattca
    12121 gacgctgatc ttccttgtat cgggccagtg tgaagacgga gtcgacactg atgccgagca
    12181 cctgggcgtt agcttcgttg tagagggata tattatcgcg tgtgctgcaa agctcttttg
    12241 tgcaggtgcc ggtgaaggcc tgcggaaaga agaggagcag gacattcctg cctttaagat
    12301 cgctgaggtt cactttttgt ttgtcggagt cgtagagcgt gaaatcgggt
//
LOCUS       DASYSV010000029         7228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1160048, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000029 DASYSV010000000
VERSION     DASYSV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160048"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..2553)
                     /locus_tag="VGQ51_00815"
     CDS             complement(169..2553)
                     /locus_tag="VGQ51_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035120.1"
                     /translation="MIRNHLRAAWRNCWAHRSVTLLNIAGLSAGMTAAILIFLWIGNE
                     TSYDSYHPGADRIYRLTSTLRKINRKWGTAPLPLADDIRAELPQVEKTSVIKSSYSTW
                     FRIGDEFFEEKKAAYVGKDWFELMHYDFVEGDAASFMAHPFSLALTEDKAKQYFGNRS
                     AIGRTIRIDTADYEVRAVIKNNPSNSSFQYDVLMPLDAWLSSPLIRHGEMTYDNYNYA
                     MFVRLREGTDPVKTAAKITSVLNSHQKGFSRIDLEPLRDMHFDKDMSTASVATIDRST
                     VYIFSALGIFLLVIACINYVNLTTARASLRAKEVSVRKIVGADKRGLFVQFILESLLV
                     SVLALVITLLLVRLSLPLFDSLTGKRFADPLTSPVLWKIVAITLLTATALNGVYPALF
                     LSSFNPLNVFRGSSLPGFRDVSLRKGLVILQFTFSIILVIATIVIRRQLDYIQTTDPG
                     YNRAQVFSFSLPWTTFVNKPAGTKTAMFTGILHDLSQQSGIADVCMASESLVNLRSSN
                     SSSADWEGHDTSYKPTVYQLSADEDYRRFFDIRLAAGRWFEPANAADQHNFILNETAV
                     NDFGMHKPVLGQRFSFQGDTGTIIGVVKDFHYASMHTRIAPMVIFNRTAWRNTFFVKA
                     QPGHAAGALAFAKTMMRRYNPGHPFIYSWLDDEFATLYRADQKLSGLILTFSVIAVVI
                     SCMGLFGLAAFAAGQRKKEIGIRKILGASVSTILLLLSRDFIRLVLVSIIVATPLAGW
                     MMYRWLQDFAYRVPLGAGIFVLAAVLAVGIALLTISFQSVRAAMANPVDNLRTE"
     gene            complement(2636..6760)
                     /locus_tag="VGQ51_00820"
     CDS             complement(2636..6760)
                     /locus_tag="VGQ51_00820"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HEV9035121.1"
                     /translation="MGKLYLLLLAALLPALAQAQIVWDFSTATPTSGIPAGVAISAVT
                     QGNNNGTTTLLTTTSASSGYTGATGGNNAGAAANVGAFRPDTSTFFEWTLTPVAGVSV
                     TVNQVSFGSRSTGTGPQAYDIRTSLDGFASAAATATLANNSAWALLANPLSLAGASGQ
                     PITLRIYAYNGTGSASKNTANWRIDDLSVGVATSGSIPVPTVTASPIPLTGFTAVSGS
                     PSATLPLTIGGTNLTAGITVAASQWYEISRDGVTYGSSATLQQIGGVVADTPILVRLA
                     AGTPPGIIQGAIILRSTGADSAAVPLSGSVTAPPAAIVVNQVYGGGGNSGSLFSNDFI
                     ELFNDGDTAVNLSGWSVQYASATGSSWQVTPLGAIIPPHGFFLVGESQGANATAPLPS
                     PDVSGTISMSATSGKVLLSNSIAAQSGANPAGPTVIDIVGYGPTATGFEGAPAPVLTN
                     STADLRTPDGHNTGNNSADFVATAPNPRNAAYTTQPPLVQTLVPGNNANLIPSGTIPI
                     LVFNKAVAKGDGSITLVTNGVNGTPIPVSDASFVVSNDTVVLHLPIAGGNRYSILVSS
                     GAFVDVFGNAFPGLNSVTDWTFTTFNNTIAQVLPFTTSFDSCLGSGQLPNGFTAYSIT
                     GAQVWDCTTFGRDSTNLSGTTPFGHAVEINGFASGINNANQDWLISPRFDLSGTAFPL
                     LGYWSRNAFAGAPLMLKISTDYTGSGDPTLAHWTDVNGKFPSQGSDVWTSSANIDLAA
                     FKDTSVYIAFVYTSSTQDGSRWTVDDISLVNSLTPPPPSLTLSTGNIEFGYTAAHTPA
                     TQKLIVTGNDLTGNITITSGGSFRVSADSIHFSDSVTLPAATANNTAEPVFIRFTPSS
                     TNIQFVDSVVVAISDSTAVVNVKGNSIDPASTLSVIDWNLNWFGTPDPTLGPTDKALQ
                     KKNVGLILPALHADLYALEEVCDEQALDSIVATMPGYAVVVGEYGSFSNPTIPGGPDP
                     LNTVQKLAFVYNTAKISAIRTDSLLTLGVTNPLDPFTTYYNDWASGRFPYMLTANVTL
                     SDNKGGTNTRIIRFIDVHAKANTSPVLTAYARRADGADALDSLLKAQYPNDLLVILGD
                     FNDDLNTTITAGVNPPVTSWSAFTIDDSALYKFPTQPLSPAGQHSDVNFTSVIDNVIL
                     SDTMNTYYLPGSATVRSDVATVVTGYGTTTTDHYPVFTQYSFTPPGSGPVLPPLTFSA
                     TKQGNTALLKWTTTPDNLTKWFEVQKSLDGRLFLPIGLIPAKSSNGIDPSTDYSFVDL
                     LPLPGTDYYRLKEFDSTRASTLSNTVSLTFTPPLTVVILPNPAIGFTEVIVTHADGPY
                     VIQVLNSNGGILKQVGGLPGNPINTVGLSGLRAGVYTVKVTTSDAVATAKLLVL"
     gene            6854..>7228
                     /locus_tag="VGQ51_00825"
     CDS             6854..>7228
                     /locus_tag="VGQ51_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9035122.1"
                     /translation="MDNHPTDVALRYIVNDVDAAIAFYTSFLNFKVEMHPAPAFAILS
                     RGNFRLLLTKPGPGGGGQGMQDGSVQAPGGWNRIHIVVEDLEATINALKEKNAKFRNA
                     MVIGVGGNQILLEDPSGNLVELF"
BASE COUNT         1566 a         1897 c         2270 g         1495 t
ORIGIN      
        1 cagaaaatat accccatagt gatagccatg caaaactttt cgtctgaaaa accgaaacca
       61 tttacgcata tcctgcaaag atacggttat ggcggcggac gcccatcggc ggaaggcacc
      121 ttactcatac cgtctggttc ctctcgggag tgccggctcg cccctccctc attcagtccg
      181 caaattatcc acggggttgg ccatcgccgc acggacagac tggaagctga tggtcagcag
      241 cgcgatcccg acagccagga cggcggccag gacgaaaatg ccggcgccca gcggaacccg
      301 ataggcaaag tcctgcagcc agcgatacat catccacccg gcgagcggcg tggcgacaat
      361 gatagagacc agcaccaacc ggataaagtc gcgcgacaac aaaagcagga tagtactgac
      421 cgaggcgccc agtatcttcc ggatgcctat ctccttttta cgttgtccgg cggcaaaggc
      481 ggcgagaccg aacaagccca tacaggatat gacgaccgcg atgaccgaga aagtaagtat
      541 gagacccgac agcttctggt cggcgcgata gagggtcgcg aactcatcgt cgagccagct
      601 atagataaag ggatgccccg ggttgtaccg gcgcatcatc gttttggcga aggcgagggc
      661 gccggcggca tggccgggtt gggcttttac aaaaaaggta ttgcgccagg cggtccgatt
      721 gaagatcacc atgggcgcga tacgggtatg catgctggca taatgaaagt cttttacgac
      781 tccgatgatc gtcccggtgt cgccctggaa actgaaacgc tggccaagaa cgggtttgtg
      841 cattccgaaa tcattcactg ccgtctcgtt gagaatgaaa ttgtgttgat ccgcagcatt
      901 ggcgggctcg aaccatcggc ccgctgccaa ccggatatcg aaaaaccggc gatagtcctc
      961 gtcagccgac aactgataca cggttggttt atagctggtg tcgtgtcctt cccagtcggc
     1021 ggagctggaa ttggaggatc tcagattgac gagggactcg ctggccatgc acacatcggc
     1081 gatgccgctc tgttgtgata gatcgtgaag gatgccggtg aacatggccg ttttcgtccc
     1141 ggcaggctta ttgacgaatg tggtccaggg cagactaaaa gaaaacacct gggcccgatt
     1201 gtaaccggga tcggtcgttt ggatatagtc gagctggcgc cggatgacga tggtcgcgat
     1261 gacgaggatg atggagaaag tgaactggag gatcaccagg cccttgcgca aagaaacgtc
     1321 cctgaaaccc ggcaggctcg agcctctaaa gacattcagc gggttgaagg aggaaaggaa
     1381 cagcgccgga tagaccccgt tgagcgcggt ggccgtaagc agcgtgatcg ctacgatctt
     1441 ccaaaggaca ggcgaggtca gcgggtcggc gaatctcttc ccggtcagcg agtcgaataa
     1501 cggcaggctc agccggacca ggagcagcgt gatcacgagc gcaaggacgc tcaccagcag
     1561 cgattcgagg atgaactgta cgaacagccc ccttttatca gcgccgacga tcttgcggac
     1621 gctgacctct ttggcgcgca ggctggcgcg tgcggtggta aggttgacat agttgataca
     1681 agcgatgacc agtagaaaga tgccgagcgc gctaaaaata tagaccgtcg accggtcgat
     1741 ggtcgcgaca ctcgccgtcg acatgtcttt atcgaaatgc atgtcgcgca gcggttcgag
     1801 atcgatgcgc gaaaagccct tctgatggct gttcaggacg ctggtgatct tcgcggccgt
     1861 cttcaccggg tcggtccctt cgcgaagtcg gacaaacatg gcgtagttgt agttgtcgta
     1921 cgtcatttcc ccgtgacgta tcaatgggct ggacagccag gcatccaggg gcatgagtac
     1981 gtcgtattga aagctggagt tggaaggatt gtttttgatg acggcgcgta cttcgtagtc
     2041 ggcggtatcg attcggatcg tccgaccgat ggccgaccgg ttgccgaaat attgcttcgc
     2101 cttgtcctcg gtgagcgcga gactgaaggg gtgcgccata aaagatgcgg catcgccttc
     2161 gacgaagtca tagtgcatca gctcgaacca gtctttgccg acataggccg cctttttttc
     2221 ttcgaagaat tcatcgccga tgcggaacca cgtactgtag gaagatttga tgacggaagt
     2281 cttctccacc tgcggtagct ctgcgcgtat gtcatcggcc aggggaagag gagcagtgcc
     2341 ccatttcctg ttgatcttgc gcagcgtaga cgttaaccgg tagatgcggt cggcgccggg
     2401 gtgataggag tcatagctgg tctcgttgcc tatccaaaga aagatcagga tggccgcggt
     2461 catgcccgcg gacagaccgg cgatgttcag gagggtgacc gatcggtgag cccaacagtt
     2521 gcgccaggcg gcgcgcaggt ggtttcggat catgggtaaa aaagatgggt gagcattaaa
     2581 gatacgctca cccatcgaaa tgttgcatcc gcccttcggg cagccgcctc tcgggttata
     2641 aaaccagcaa tttagccgtc gccacggcat cggacgtcgt aacctttacg gtgtagactc
     2701 ctgcgcgaag accgctgagg ccaaccgtat tgatcgggtt gccgggcagg ccaccgacct
     2761 gtttgaggat gcctccattg gaattcagca cctggataac gtagggaccg tccgcatggg
     2821 tcacaatgac ctccgtgaac ccgatggcgg ggttgggaag gatcaccacc gtcaatggcg
     2881 gagtgaatgt aaggcttact gtattggaaa gcgtggatgc tctggtcgaa tcgaattcct
     2941 tcagcctata gtagtcggtg ccgggcaagg gcaacagatc gacgaagcta tagtcggtgg
     3001 acggatctat accattgctg ctcttcgcgg ggatcagacc gatcggaagg aatagccggc
     3061 catccaggct tttttgcacc tcgaaccatt tggtcaggtt gtcaggcgtg gtcgtccatt
     3121 tcaacagcgc ggtatttccc tgcttcgtcg cgctaaaggt cagcggcggc aagacgggcc
     3181 cgcttccggg aggggtgaaa gaatactgcg tgaataccgg gtagtggtcc gtcgtagtgg
     3241 tgccatagcc ggtgacaacc gttgcgacat cggatcgcac cgtagccgat cccggcagat
     3301 aataggtatt catcgtgtca ctcaggatca cgttgtcgat gacgctggtg aagttgacat
     3361 cggaatgctg accggccggg ctcagcggct gggtcgggaa tttatacagc gcggagtcat
     3421 cgatcgtaaa ggcgctccag gaggtgaccg gcggattgac gcccgcggtg atggtcgtat
     3481 tcagatcgtc attgaagtcg cccaggatga ccaggaggtc gttgggatat tgcgctttca
     3541 gcagcgagtc gagagcatca gcgccatcag cgcgacgcgc ataggccgtc agtaccggcg
     3601 aggtgttcgc tttggcgtgt acgtcgatga aacggatgat ccgggtattg gtcccgccct
     3661 tgttgtcgct gagcgtaacg ttggccgtaa gcatgtacgg gaaccggccg ctcgcccagt
     3721 cattataata ggtggtgaaa gggtccagcg ggttcgtcac gcccagggtc agcagtgagt
     3781 ccgtccggat cgccgaaatt ttggcggtgt tgtagacgaa ggccagtttc tggacggtat
     3841 tcagaggatc cggtcctccc ggtatggtgg gattcgagaa ggaaccgtat tctcctacga
     3901 ccacggcata tccgggcata gttgcaacga tggaatccaa cgcctgttca tcgcatacct
     3961 cttccagggc atacagatcg gcatgcaggg ctggcaggat gagacccaca ttctttttct
     4021 gcagcgcctt gtcggtgggt ccgagtgtcg gatcgggggt gccgaaccaa ttgaggttcc
     4081 agtcgatgac gctcagcgta gaggcgggat cgatgctgtt tcccttgaca ttaacgacgg
     4141 ccgtcgagtc ggagatggcg acgacgacgg agtctacgaa ctggatgttg gtggaagacg
     4201 gcgtgaagcg gataaacacg ggttcggcgg tgttgttcgc cgtggccgcc ggcagggtga
     4261 cgctatcgct gaagtgtata ctgtccgcag acacccggaa gctacccccg gaggtgatcg
     4321 tgatgttccc cgtgagatcg ttgcccgtaa cgatcagctt ttgcgtggcc ggggtatgcg
     4381 ctgccgtata gccgaattcg atgttaccgg tgctcagcgt gaggcttggc ggcggcggag
     4441 taagcgagtt gaccaggctg atatcgtcca ccgtccaacg cgagccgtcc tgtgtacttg
     4501 acgtatagac gaacgcgatg tacacggaag tatccttgaa agcggccagg tcgatgttgg
     4561 cggacgaggt ccagacgtcc gatccctggc tcgggaattt tccgttcacg tcggtccaat
     4621 gcgccaacgt gggatcaccc gaacccgtat aatccgtgga tattttcagc atcagcgggg
     4681 ccccggcaaa agcattgcgg ctccagtagc cgagcagggg gaaggccgtt ccgctcagat
     4741 cgaacctcgg cgagatcagc cagtcctgat tggcattgtt gataccgctc gcgaagccat
     4801 tgatctcaac ggcatggccg aaaggcgtgg tgcccgaaag gttcgtgctg tccctgccga
     4861 aagtagtaca gtcccatacc tgtgcgcctg tgatactgta ggcggtgaag ccattgggta
     4921 gctggccgct tcccaaacaa ctgtcgaaac tggtggtgaa gggcagcacc tgcgcgatgg
     4981 tattattgaa ggtcgtgaat gtccagtcgg taacggaatt caacccggga aacgcatttc
     5041 cgaacacatc cacgaacgcg ccggatgaaa cgagtatcga atagcggtta ccccccgcaa
     5101 tgggaagatg gaggacgacc gtgtcattgc tgacgacgaa gcttgcatcg ctgacgggta
     5161 tcggcgttcc gttcactcca ttggtcacca gcgtgatgct gccgtcgcct ttggcgacgg
     5221 ctttattgaa taccaggatc gggatcgtgc ccgacgggat aaggtttgca ttgttccccg
     5281 gcaccagcgt ttgcaccagc ggcggctgcg tggtataggc ggcgttccgc gggttgggag
     5341 ccgtcgccac gaaatctgcg gaattgttgc ccgtattgtg accgtcggga gtccgcaggt
     5401 cggcagtgga attggtcagt accggcgcag gcgctccttc gaagccggtg gccgtgggtc
     5461 catatccgac gatatcgatg acagttggac ccgcagggtt ggcgcccgac tgggcggcga
     5521 tggaattact cagcaggacc ttaccggaag tagcgctcat cgagatggtc ccggagacat
     5581 cgggtgacgg caacggcgcg gtcgcatttg cgccctgcga ttcccctacg aggaagaacc
     5641 catgcggggg gatgattgcg ccgagcggtg tgacctgcca ggaactgccg gtcgcggaag
     5701 catattgaac ggaccagcct gaaagattta ctgccgtgtc gccatcatta aacaactcaa
     5761 tgaagtcatt ggagaaaagc gatcccgaat ttccgccgcc gccatatacc tggttgacga
     5821 caatagccgc aggcggtgcg gtgaccgacc cggaaagggg gactgccgct gaatcggcgc
     5881 ccgtggagcg gaggatgatc gctccttgta ttattcccgg cggagtgccg gccgccagcc
     5941 ggacgaggat gggcgtgtcc gctacaacgc cacctatctg ttgaagtgtg gccgagctgc
     6001 catatgtcac accatcccgg gaaatctcat accactggga ggcggcaacc gtgatacccg
     6061 ccgtcagatt ggtcccaccg atggttagcg gcaatgtggc cgacgggctg ccggacactg
     6121 cggtaaagcc ggtcaggggg ataggtgagg cagtgacagt gggaaccggg atgcttcccg
     6181 acgtagcaac gccgacgctc aggtcatcga tccgccagtt ggccgtattt ttcgaggcgc
     6241 tgcccgtacc gttgtatgca taaatgcgca gtgtgatggg ctgtcccgag gctccggcga
     6301 gtgaaagggg gttggcgagg agagcccagg cgctgttgtt ggcgagtgtg gcagtggccg
     6361 ccgcgctcgc gaaaccgtcg agactggtgc ggatgtcata ggcctgtggt cccgtgcccg
     6421 tactccggct gccgaaactg acctggttca cggtaacgga cacacccgca accggcgtca
     6481 gcgtccattc gaaaaatgtg gaggtgtcag gccggaaggc tcccacattg gccgccgcgc
     6541 cggcattgtt gcccccggtg gcgccggtat accccgatga ggcggaggtt gtggtcagca
     6601 gtgtggtcgt gccgttgttg ttaccctgtg ttaccgccga aatggcaact ccggccggga
     6661 taccggaggt cggggttgcg gtcgaaaaat cccatacgat ctgagcttga gcaagtgcgg
     6721 gcaataaggc tgccagcaac aataggtaaa gttttcccat tacagtcagt tttaattttg
     6781 aggagttcag gacaaattac cgaaaattgg agcgattttg ggtttgaaga aattattaac
     6841 aaatttaccc gccatggata atcacccaac cgacgtggcc ctcaggtata tcgtcaacga
     6901 tgtagatgcc gctatcgcct tttataccag ctttttgaat ttcaaagtgg agatgcatcc
     6961 ggcccccgca tttgccatcc taagccgggg caactttcgt ttgttgctga ccaagccggg
     7021 cccgggaggc gggggtcagg ggatgcagga cggatcggtc caggctccag gcggctggaa
     7081 ccggatacac atcgtggtgg aggacctcga agcgacgatc aatgccctca aagaaaaaaa
     7141 tgcaaaattc cgtaatgcga tggtgattgg ggtcggcggg aaccagatcc tgctggagga
     7201 cccgtctggg aacctggttg aattattc
//
LOCUS       DASYSV010000030         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_647311, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000030 DASYSV010000000
VERSION     DASYSV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647311"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..201)
                     /locus_tag="VGQ51_00830"
     CDS             complement(28..201)
                     /locus_tag="VGQ51_00830"
                     /inference="COORDINATES: protein motif:HMM:NF039278.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HEV9035123.1"
                     /translation="MAIEIREVTIKATVQQETGGAGKQGNAGVNNSVGSNEEVLQTAI
                     EKVLDILKDRNER"
     gene            complement(204..671)
                     /locus_tag="VGQ51_00835"
     CDS             complement(204..671)
                     /locus_tag="VGQ51_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV9035124.1"
                     /translation="MGTTIQGDYYPNVNFHFMVQFPSLDPTGTIDVAFQSVSGLDSTL
                     ETEQVKEGGENRFTHVVPVRRKFGPLVLKRGLVGPSSPLTQYLQYVFANDAFDPLDVV
                     TVQLLDETHQTLMSWSVNNVWPLSWKLSDLNAMQGEILIETLELNYNQLVLNS"
     gene            complement(684..1148)
                     /locus_tag="VGQ51_00840"
     CDS             complement(684..1148)
                     /locus_tag="VGQ51_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV9035125.1"
                     /translation="MAASLDPTKAYPLPKFHFQVSWGEGTMSFTEVSGLDFETEVIEY
                     RAGDAPTYNKTKQPGLNKYSNVTMKKGIFLGDYDFYNKWLKTFYFQEQNTQYRTTVVI
                     QLLNELHTPVITWNLYKAWPTKITSTDLKADANEVAIETMEWVHEGLSIVEA"
     gene            complement(1224..2177)
                     /locus_tag="VGQ51_00845"
     CDS             complement(1224..2177)
                     /locus_tag="VGQ51_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035126.1"
                     /translation="MLNEFRHECRPRWDIEKIHRCCPEPPPSHEPPCNPDCCYEVWTW
                     EQTHVNIKLKDVGSELADAQKHLTVVTARYSRLKTWHDELTKANELSFTICRQLKLIE
                     CQLESICKNTCFTVHAVEILYCMIREFYYTVDCLQEKYDRLMNCLKSLNIPALNTTSG
                     IGKVLSDYGAALTAVIGTRAALIALMMKVVDGALKLHDELCDDCGYQLLIRHCRESLH
                     CGIPCEEVEHHHPHPLPLPSPQPGYGPPQPGAEEFCLAPMLQFPICNDPYYRLIADLY
                     RDEKRVVDEVTEQVNELTKEQVALTAIQQSLTKALKEVTPA"
     gene            complement(2215..3276)
                     /locus_tag="VGQ51_00850"
     CDS             complement(2215..3276)
                     /locus_tag="VGQ51_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035127.1"
                     /translation="MDTRANTYPGNYPGNQPGPGHHPERECKEKDTQTGKAIDTARLT
                     YCTNLQTAKGDQASSEKAYDRSVMIYHKKKKLFEWTEHNYRLYRDFDICVDNELTAVT
                     TSLTANVKAISGLSGDLFTNLKSVVTKIADLKTKIGALGDAAYNLDKYKNDQANATQW
                     CLLTGKNVDNCRPEHEHHHHRPEHCHDADQIYHELIHLTKKVLIADVNSLVQSAADTA
                     GIQTFTNIDTLTTQQTAFTTAATALVTQIQTTVTTRRKDLDGVQADLSAAVQACTSAG
                     VDKYTKTSIAHAAHCTVQFLCCPDCECVRIEPCDSHEPRLIDCECRICDIGEKIRSTY
                     RGEGDPDPDCGCHGKEPRR"
     gene            complement(3313..>3528)
                     /locus_tag="VGQ51_00855"
     CDS             complement(3313..>3528)
                     /locus_tag="VGQ51_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV9035128.1"
                     /translation="MIGNYLTGLWKQGALMGNTAKESFYVHVGLGETMVETDIWEGRM
                     IVQIGMAVVRPAEFIILQFMQMMQSPS"
BASE COUNT          686 a          941 c          985 g          916 t
ORIGIN      
        1 ttcgtaacgg cgctgccgaa gggagtacta tcgctcattg cggtctttta aaatgtccaa
       61 cactttttcg atggcggtct gcaacacctc ctcattggag ccgaccgagt tattcacgcc
      121 tgcgtttcct tgcttcccgg cgccgccggt ctcctgctgg acagtcgcct tgatggtcac
      181 ttcgcgtatt tcgatagcca tattcaggaa tttaggacta gctgattata gttcaattca
      241 agcgtttcga tcaggatctc gccctgcatc gcattgagat cgctgagctt ccagctcaac
      301 ggccagacat tgttgaccga ccaggacatc agcgtctgat gcgtctcatc cagcaattgc
      361 accgtcacca cgtccagcgg atcgaacgca tcgtttgcga atacatactg caaatattgc
      421 gtcagcggag acgaaggacc tacgaggccg cgtttcagga ccagcggacc gaatttccgc
      481 cggaccggca cgacatgggt gaaccggttc tccccacctt ccttcacctg ttcggtctcg
      541 agcgtggaat ccagacccga caccgactga aaggcgacgt cgatggtccc cgtggggtcc
      601 agcgaaggga attgtaccat gaagtgaaaa ttcacattcg gataataatc tccctggatc
      661 gttgttccca tgactatatc tttttatgct tctacgatcg aaagcccctc atgaacccac
      721 tccatcgttt cgatggccac ctcgttggcg tccgccttca ggtcggtgga ggttatcttc
      781 gtcggccacg ctttgtacag gttccatgtg atgacgggag tatgcagctc gttcaggagc
      841 tggatgacga ccgtggtccg gtactgcgta ttctgctcct ggaaatagaa ggtcttcaac
      901 catttattgt agaagtcgta gtccccgagg aagatgccct tcttcatcgt tacattgctg
      961 tatttgttca aaccgggctg tttggtcttg ttataggtgg gagcgtctcc ggcgcgatac
     1021 tctatcacct ccgtttcgaa atcgagaccc gaaacctcgg tgaaggacat cgttccttct
     1081 ccccagctca cctggaaatg gaatttggga aggggatagg cttttgtggg atctaatgat
     1141 gctgccataa atgcgcgatt ttaaaaagat ttaaaaattg gttaggccgc acgcgtggcg
     1201 cggacgacag gccggcgcaa ggctcaggcc ggcgtcacct ctttgagcgc cttcgtcagc
     1261 gattgctgaa tggcggtgag ggccacctgt tccttcgtca gctcattgac ctgctcggta
     1321 acctcgtcga cgactctctt ttcgtccctg tacaagtcgg cgatgaggcg ataatacggg
     1381 tcattgcaga tgggaaattg cagcatcggc gcaagacaga attcttcggc gcccggctga
     1441 ggcggtccat atcccggctg cggcgatggc agcggcaggg gatgagggtg atggtgttct
     1501 acttcctcgc acgggatacc gcaatgcagc gactctctgc aatgacggat caacagctgg
     1561 tatccgcaat cgtcgcacag ctcgtcatgc agcttcagcg cgccgtcgac tactttcatc
     1621 atcagcgcga tgagcgcggc acgtgtcccg atgacggccg tcagtgcggc gccatagtcc
     1681 gaaagcacct tcccgatacc cgacgtggta ttcagcgcgg ggatgttcag ggatttgaga
     1741 caattcatca gccggtcata tttctcctgc aggcaatcca cggtataata gaattcgcgg
     1801 atcatgcaat agaggatctc taccgcatgg acggtgaaac acgtgttctt gcaaatgctt
     1861 tccagctggc attcgatgag cttgagctgg cggcagatgg tgaaagaaag ctcgttggcc
     1921 ttcgtgagct cgtcatgcca ggtcttgagc cggctgtacc gtgccgtaac gacggtgaga
     1981 tgtttctgtg cgtcggccag ttcgctgccg acgtccttga gcttgatgtt cacgtgtgtc
     2041 tgctcccagg tccatacttc gtagcagcaa tcgggattgc atggcggctc atgagacggg
     2101 ggcggctcgg ggcagcatcg gtgtatcttc tcgatatccc agcggggacg acactcatgt
     2161 ctgaattcgt tgagcatata aaatgaattt tagattgttg gaataatgac ggttttatct
     2221 tctcggttct ttgccatgac acccgcagtc cgggtccggg tcgccttccc cgcggtaggt
     2281 cgatctgatc ttctccccga tgtcgcagat cctgcattcg cagtctatga ggcggggttc
     2341 gtgcgagtcg cagggttcta tcctgacgca ttcgcaatcc ggacaacaga ggaattgtac
     2401 cgtacaatgt gcggcgtggg cgatgctggt cttggtatat ttatccactc cggcgctggt
     2461 gcaggcctgt acggcagctg acaggtcggc ctgtacgccg tcgaggtctt tccgccgggt
     2521 cgtgacggtg gtttgtatct gggtcacgag cgcggttgcg gccgtcgtga acgccgtttg
     2581 ctgtgtcgtc agcgtatcga tattggtaaa ggtctggata cctgccgtat cggctgccga
     2641 ctgtacaagc gagttcacgt ctgcgatcag gaccttcttg gtgaggtgga tcaattcgtg
     2701 atagatctgg tcggcatcgt ggcaatgctc cggccggtgg tgatggtgct cgtgttcggg
     2761 tctgcagttg tccacattct tcccggtcag caggcaccat tgtgtggcgt tggcctgatc
     2821 gttcttatac ttgtcgagat tgtaggccgc atcgcccagc gctccgatct tggtcttcag
     2881 atctgcgatc ttggtgacca cggatttcag gttggtaaag agatcaccgc tcagcccgct
     2941 gatggccttc acgttggcgg tcaggctggt ggtcacggcc gtcagctcat tgtcgacaca
     3001 gatgtcgaaa tccctgtaga gccggtaatt gtgttccgtc cattcgaaaa gtttcttttt
     3061 cttatggtag atcatcacgc tccggtcata ggctttttcc gaagaggcct gatcgccctt
     3121 ggccgtctgg agattcgtac agtaggtcag tcgcgcggtg tcgatggcct ttcccgtctg
     3181 ggtatccttc tccttgcatt cccgctcggg atgatgaccc ggacccggtt gattgccggg
     3241 gtagttgccg ggataagtat ttgccctcgt gtccataata ttaggtttaa ttggtaaata
     3301 atgctgttac gatcaggaag gactttgcat catctgcatg aactgcagga tgatgaactc
     3361 ggcaggtctg acgacggcca ttccgatctg gacgatcatc cttccctccc agatgtccgt
     3421 ttctaccatg gtctcgccca ggcccacatg gacatagaag ctctccttgg ctgtattccc
     3481 catcagggca ccctgtttcc agagaccggt gagataattg ccgatcat
//
LOCUS       DASYSV010000031        16969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1288292, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000031 DASYSV010000000
VERSION     DASYSV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 16969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16969
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288292"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..507
                     /locus_tag="VGQ51_00860"
     CDS             85..507
                     /locus_tag="VGQ51_00860"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV9035129.1"
                     /translation="MKRTATAVWNGSGKEGKGQLTTQSTTLNKTQYSFKTRFEDGVGT
                     NPEELIGAAHAGCYAMALAFALQNAGFTADSLEVTATINFETATPPPTITQSALVLRA
                     KIPGITDDKFQELAKGAKEGCPVSRVLKAEVTLDAKLV"
     gene            593..2005
                     /locus_tag="VGQ51_00865"
     CDS             593..2005
                     /locus_tag="VGQ51_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9035130.1"
                     /translation="MSTKRKNSRRGFIRDTSMAATGFFIVPRHVLGKGFLAPSDRLAI
                     AAIGAGGKGGDDLRNFYGSGKVDVAFLCDVDDRQAANSVKTYTKAKYYKDFREMLDKE
                     HKHFDAVSVSVPDNCHAVAAMAAMQLGKHVYVQKPMTHDIYEARTMTEAAKRYKVVAQ
                     MGNQGSSGDGVRLMQEWYNAGLIGDATAIHVWTNRPVWPQGGLKPTTKDEIPKELDWE
                     LWQGPAAADEYHKEFLPFNWRGWWAYGTGALGDMGCHLIDPAFKTVGLGYPSEVECSM
                     STLYQVMWTPLAHPDSCPVSSSVKMKFPGKPGKPDVALYWMDGGIQPERPDELGPNEQ
                     MGGSDGGVIITGTKGKMMCGTYGANPTLLPTTRMSETANVPKTLARVPEGHYVQWVNA
                     CIAGYGQKELSSPFDYAGPLTETILMGNLALRSFNIRDTSGHYPGRKKLLWDAANMKI
                     TNFDEANQFVKREYRQGWKL"
     gene            complement(2051..2923)
                     /locus_tag="VGQ51_00870"
     CDS             complement(2051..2923)
                     /locus_tag="VGQ51_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV9035131.1"
                     /translation="MQENREILALFHLIDDPDQEVFDSVSNRIVSYGRGIIPNLENLW
                     ENTISGDVQERIELLIHKLHYHDLTEDFQHWKSSSYQDLLTASLLIAKFQYPELVTTP
                     VLHEVEKLRRNIWLELNSYLTPLEQVHIVTSILYNYYGLKGTEVAYPHPEEFLINKLI
                     ETKRGNSITNGILYLLLCELLDIPVKAINIPRQFVLAYIKPYYDENPEKLSPMQRIEF
                     FIDPTSGQVFSHKDVESYFKRISVPPVASYFKPLSHYRIIQTALEEYSKCFEDERNAY
                     KQKELLELAKLLNE"
     gene            complement(3088..5433)
                     /gene="topA"
                     /locus_tag="VGQ51_00875"
     CDS             complement(3088..5433)
                     /gene="topA"
                     /locus_tag="VGQ51_00875"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV9035132.1"
                     /translation="MAKNLLIVESPAKAKTIEKILGEDFEVKSCYGHIRDLEKDDMGI
                     DIKNNYRPRYIVPEDKEKVVKELKSVAKKSGEVWLATDEDREGEAISWHLCEVLGLDP
                     ATTKRIVFHEITKPAIQSAVKRPRTVDMNLVNAQQARRVLDRIVGFELSPVLWRKMSM
                     KNNLSAGRVQSVAVRIIAEREREINAFTAVSSFKIEALFTAKDISGKTVTFSAEGRKQ
                     SSSDNAQKFLQGCIGAGYAVTDIQVKPGKKTPAAPFTTSTLQQEASRKLGYSVSRTML
                     LAQKLYESGKITYMRTDSVNLSELAMDAAANQIRSQYGDKYHQARKFKNKNESAQEAH
                     EAIRPTYIDALTVEDADCRRLYDLIWKRTMASQMADAETEKTIAKIQISTNKEDLTAS
                     GEVLKFDGFLKVYMEDRDEDDGSEEEAQESMLPPLRVGQALPLKEMRAVERFTRPQPR
                     YTEASLVKKLEELGIGRPSTYAPTISTILKRGYVEKRDKEGLKRDLRILVLKNEHIET
                     LAEQETYGSEKSKLFPTDLGLVVTDFLKQYFDDIMDYHFTARIEEEFDEVAQGKMLWS
                     KMIDDFYNPFKRDVENTIENAERIKGERELGIDADSGKRVVARMGRYGPMVQIGDVND
                     EEKPRFAKLKNTQSIETISYDEAMELFRLPRNLGIFEENEVTVNIGRFGPYIAHDGKF
                     YSLGKEFDPYTITFDQATPIVVEKRAAKEQRTIKVFEKEKIQVLRGPYGPYIKQGLRN
                     YKLPKEKQEAAADLSLDEVKAIIEEVKANPPKRVPRRKKGS"
     gene            5692..6438
                     /gene="pxpB"
                     /locus_tag="VGQ51_00880"
     CDS             5692..6438
                     /gene="pxpB"
                     /locus_tag="VGQ51_00880"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEV9035133.1"
                     /translation="MAGSFSYSIFPLGDSAVTLDIGDGKGIDEQNNIRALALHDWLQT
                     HRFPGIVDIIVAYSSVSVFYDTAVVITQDQTAFSRIKALLTQAWQENDGVVPATVVAE
                     GRLIRLPVCYEAAYAPDLEWVARETGLSCEEVINIHCAGPYRVYMIGFLPGFSYLGRV
                     DPRLELPRKKFPVTVQAGGVGIAGMQTGVYPLNSPGGWQIIGRTPVRLFDPGMDPPVV
                     LQTGDRVQFYPVGDDEFHRIRASGIVSRFA"
     gene            6579..7571
                     /locus_tag="VGQ51_00885"
     CDS             6579..7571
                     /locus_tag="VGQ51_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEV9035134.1"
                     /translation="MAFSIIKPGLLDTIQDMGRQGFGSWGVSPGGAMDGYAAQVANLL
                     VGNDKHEAVIEIHFPGPQILFEQNTLIAITGADFSPTVNDEIIPRWQPVVVRKNTVLH
                     FPSLVQGGRCYLSVHGGYCLKKWLNSYSTSLKAGAGGWGGLPLKKGDELPFNESQIYF
                     AGLLKEENNFEVLPWRVNMEKTYQFPHEIGFLPGHEWPLLSDAARHSFLEDNYIIHPS
                     SDRMGYQLSGAPIFPDQPIELVSSAVSFGTVQLLPSGQLIVLMADHQTTGGYPRVAHV
                     VSAHLPKLAQLRPSDSIQFKLMDTEAAEQLLVCRQKELHILQRSCMDHLNEMVC"
     gene            7565..8389
                     /locus_tag="VGQ51_00890"
     CDS             7565..8389
                     /locus_tag="VGQ51_00890"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEV9035135.1"
                     /translation="MLRSPGGAGGAQSEEPADGVRLPAIDLNCDMGEGMNTDASIFPF
                     ITSANIACGGHAGDDDTMHRTVELALQYGVAIGAHPSYPDRPNFGRIDILGKTLTLAD
                     LQGVLREQLQRLQDICKAAGTRLRHVKPHGALYNRAARDQPVAAMIAQVVADFDHSLL
                     LYGLSGSEMRRSADAAGIRFVSEVFADRTYQPDGSLTPRSQPDALIDDPAKAVAQVLR
                     MTKKGNVMTFLGPVSISTETICLHGDGAHAVEFAGLIHRALTGEGIRIAAPGSSIL"
     gene            8500..9777
                     /locus_tag="VGQ51_00895"
     CDS             8500..9777
                     /locus_tag="VGQ51_00895"
                     /inference="COORDINATES: protein motif:HMM:NF025720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HEV9035136.1"
                     /translation="MATTDKTDNGRIESGYTYDSFTGVGDRAKRPTEKHFLWWCAGAH
                     QDILKDCPSEQSKYAGLGGVLLATFALAALAAGYAIYSVFSDPLWAVSFALIWGLIIF
                     NFDRFLVSTMRKYGVSRRKQIWMAAPRLVLALLIGFTIARPLELKIFEKEIDVQITAN
                     RHQKMLLNDSLLQAEQRPLLSSALQERERLNTRKSGIEDTLHQLQQSYIREADGTGGS
                     GRRGIDKLTRMKQQAYESSLTQFAPELLRLQQQTHYQDSLIAASNAGRQQRDLRYETQ
                     VTNDVGFLERNKALSDLSSRENSVFWSSLLLSLLIILIEIGPVLSKLIMNTGPYDLAL
                     ASFELRQMATAEDEMRRDKELRHAKKQGIYKAKKEMSAELLQKLSGLQKKHVQEELDR
                     WEKGEEKAEPRVSLEELARRIRDRYDFREEDVL"
     gene            complement(9812..10330)
                     /locus_tag="VGQ51_00900"
     CDS             complement(9812..10330)
                     /locus_tag="VGQ51_00900"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEV9035137.1"
                     /translation="MNKETTSIRHSKLGELFLNELRELYGAERHQLTVLPLLKKAASS
                     LKLRNVLSSHLEDTREHIHRLDQVFAKLRRVAEALPPEAIFGITREAEQVIATTPAGS
                     ATRDAGLIVAAQKMQHYEISAYGSLATYARTMDEDDIESLLELTLYEEKETDELLTAL
                     AENYINAEASRE"
     gene            10455..10703
                     /locus_tag="VGQ51_00905"
     CDS             10455..10703
                     /locus_tag="VGQ51_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035138.1"
                     /translation="MEKLKTAPVQIDGGDGPEDYVVQEKDYGDMVVYDIFRKDHYLLT
                     MARDGSILFMNFDAEPGDKEVFKLSRLNQFIEKIQAAP"
     gene            11089..12969
                     /locus_tag="VGQ51_00910"
     CDS             11089..12969
                     /locus_tag="VGQ51_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035139.1"
                     /translation="MKKAIIIRLAAALCCSWCCLSSFGQAKDTTRHDSSSHWWNRNNI
                     FQFFRNAIVKGKPDTAKDFNTLEPLNTKSETPFHPYDGKIIRHIYIRGYGFEQTFPDT
                     SRRLEYFGTQILNDLHRKTRDWVIRDALFVKENTPVDAYKLADNERLIRSLNYIQDAR
                     FLVTSLPDSPDSVDLVVVVKDLFSISGAIGALGYPPFSSRVTASEANFLGMGQRIQGG
                     MNLEQARNPNFGGQILYSKTYIGHSFTNVTASYSEINPNIYNGTPDETAWLLQLDRPL
                     YAPYAHLAGGIRVGDFQNFNVYKQPDSLFYKYHYHTRDGWIGWNIGSDRFLSNTVVLD
                     RRFIAFRYFRNDFDSIPKQVGNSFNFRFNDREAALSSLSFFRQEFYKTNYIYGFGTTE
                     DIPKGYNIALTGGWYRQLNINRVYAGVDANGYTVSKRGAFTQFFLRSGAFFSHGRPQD
                     ASFLIGASYYSPLFVLDRVKIRQYLNFSFTRLINRVGLDYLTINNVFGLRYFTNDSSS
                     GVQRATMHSETTFFLNGKILGFKFAPFAFGDVSFLTPQSGSLMKSDLWDGIGAGIRTR
                     NENLIFNTIEFRMVYFPRRAEYNNSFKILINTDIQFRYNSSYVHQPDVVFLNTDGLNS
                     IY"
     gene            complement(12963..13556)
                     /locus_tag="VGQ51_00915"
     CDS             complement(12963..13556)
                     /locus_tag="VGQ51_00915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV9035140.1"
                     /translation="MHSYLALGDSYTIGEGVPLSDSFPYQTVQLLRNGGRPFCSPEII
                     ARTGWTTGELAAAMDNTRLLARYDLVTLLIGVNDQYRGRGADEYGIGFETLLTRAMGL
                     GERLIVLSIPDWGVSPFAATGDRDPGRISQEIDVFNTTASTITRRHQVPFIDNTAHSR
                     TSTIFVADRLHPDGGTYRFWAQRLAASIIGSSAAAGQ"
     gene            complement(13556..16360)
                     /locus_tag="VGQ51_00920"
     CDS             complement(13556..16360)
                     /locus_tag="VGQ51_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV9035141.1"
                     /translation="MRKIGLLFLLMAMSAAGFSQGFPPVGGGGGRRGAGGGGQVPSIG
                     HFYGKVIDSKTGKGMDGASVQLVLTKYDPVKKAQKDSVIAGMITEHKGDFSLENLPLF
                     GNFRLKITAIGYKTYEEKVAFDLKGLRGGGARPGGMNGGVGGDNGDGQQNMAGASAAI
                     NAIDKDLGNIKLSEDAQMLESATVTASKPLITMGVDRKIYNVEKDISAAGGSATDVMR
                     NVPSVQVDIDGNVTLRNSPPTIFVDGRPTTLTLDQIPADAIQSVEIITNPGAKYDASG
                     GTAGILNIVLKKNRKAGYNGNVRAGVDERGKLNLGGDINVKQGKVNFFLAGNYGQRRS
                     INTTDQTRYSTIPQPADSLREHDKNVNDGFFAFGRGGLDYFIDNRNTLSIAGNIVHGT
                     FNPYINSDINIDTLYNGYTTSSYTGRYSTTDAHFNNHGASLSFKHNFPKAGEEWTADA
                     NYSQGSNDNLNRITSNIYNVKGGPLSSIYQQEQVGNSNNKYFTAQTDYTLPLTEKSKF
                     EAGARVAIRTNNSVNNIDTMGTDGNFHTAGLLSSQYTYQDRVFAGYVTYGNVFKNFTY
                     QVGLRGESSDYKGTQHYTAFDTSGHQLPAIGEFSSTFPISLFPSIFLTQKLGDDQDLQ
                     LNYTRRIDRPNFFQLFPFTDYSDSLNPSRGNPALVPQFTNSFELGYQKSFPGNNTLLL
                     STYYKSTTDLITRISVPGENPISHQPVYVSTYINAKSSFVGGFEIISRNTITKWWDLT
                     SNINIFTSKINTIDTGLNAIPQVGEIWSWFGKINNSFKIMSKPNKALTLQLSGDYTSK
                     TVLPPGGSASNGGGGMGRGFGGGVSGNANGYSKPSGGVDGALRFEFLKGKVASLTMSV
                     SDIFRTRINDVFTQTAFYTQEVARRRDPQFFRLQFNWRFGKFDMALFKRKNMKGDMDN
                     MQNMQQQGGGMPGGGGGGPQ"
     gene            complement(16507..>16969)
                     /locus_tag="VGQ51_00925"
     CDS             complement(16507..>16969)
                     /locus_tag="VGQ51_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEV9035142.1"
                     /translation="QRLIAESLGRIVGQELLSFGDIEQAIVNPDNVQRILPLAEEHID
                     HFLRIKLKETMPMISMFIGEKTIGQLKEVFMSELEQLFPIIMKNYVANLRHDLDLERI
                     VVEKIAGFSAHRLEALLNEFLTKEFRFVEIIGAALGFLIGLLQIALTFMMR"
BASE COUNT         3685 a         4353 c         4767 g         4164 t
ORIGIN      
        1 gaggtctatc ttctcgaaat caagccattc tatcaaagac gacatatatt aatagtttat
       61 cttaaactaa aataaataag aattatgaaa cgtacagcaa cagccgtgtg gaacggctcc
      121 ggaaaagaag gaaaaggcca attgacgacg caaagcacaa ccctgaacaa gacgcaatat
      181 tcattcaaga cacgtttcga agatggagtg gggacgaacc cggaggagct gatcggcgcc
      241 gcgcatgccg gctgttatgc gatggcgctg gcatttgcgc ttcagaatgc agggtttacg
      301 gcggacagcc tcgaggtgac cgctacgatc aatttcgaga cggcgacacc gcctcctact
      361 atcactcaat cagcactggt actgagggca aaaatacccg gtatcaccga tgataagttc
      421 caggagctgg ccaagggcgc caaagagggc tgtccggtca gcagagtgtt gaaagcagag
      481 gtcacgctcg atgcgaagct ggtgtgagtg attcccgccg aatccaataa ttagcaattt
      541 ttagcacccc cgggtgcttt ttttattagc gtgttaatgt aacttagaca ctatgagcac
      601 aaaaagaaaa aactcccgcc gcggattcat tcgcgacacc tcgatggccg ctaccggctt
      661 cttcatcgtc cccagacatg ttctcggaaa aggattcctc gcgcccagcg acagacttgc
      721 gattgccgcc atcggcgccg gcggtaaagg cggcgacgat cttcgtaatt tttatggcag
      781 cggcaaggtg gacgtagcct ttctctgcga tgtcgacgac cgtcaggctg cgaattcggt
      841 aaagacctat accaaggcca aatattacaa ggatttcagg gagatgctgg acaaggagca
      901 taaacatttc gatgccgtgt ccgtctccgt cccggataac tgccatgccg tggctgcgat
      961 ggcggccatg cagctgggca aacatgtcta tgttcaaaag ccgatgacgc acgatatcta
     1021 cgaagccagg acgatgaccg aagcggcgaa gcgttataag gtggtggccc agatgggcaa
     1081 ccagggttct tccggcgacg gggttcggct gatgcaggaa tggtacaatg ccggtctgat
     1141 cggtgatgcc actgccatcc atgtgtggac caatcgtccc gtatggccgc agggcggatt
     1201 gaagccgacg acgaaggatg agatacctaa agagctggat tgggaactct ggcagggacc
     1261 ggctgctgca gatgaatatc ataaggaatt cctgcctttt aattggcggg gctggtgggc
     1321 ttatggcacc ggcgctctcg gcgacatggg ttgtcacctg atcgatccgg ccttcaagac
     1381 cgtcggtctg ggttatcctt ccgaagtcga gtgcagcatg agcacgcttt accaggtgat
     1441 gtggacgccg ctggcgcatc cggacagctg tcccgtcagc tcttccgtaa aaatgaaatt
     1501 cccgggaaaa cccggcaaac cagatgtcgc cctgtattgg atggatggcg ggattcagcc
     1561 ggagcgcccg gacgagctgg gacccaatga gcagatgggt ggcagtgacg gcggagtgat
     1621 catcaccggg acgaaaggga agatgatgtg cggcacctat ggggccaacc cgaccctgct
     1681 gccgacgacc cggatgtcgg agacggcgaa tgtgcctaag acgctcgcgc gcgtacccga
     1741 agggcattat gtgcaatggg tgaatgcatg tatcgccggt tatggtcaaa aagagctgag
     1801 ctctcctttc gattatgccg gacctttgac ggaaaccatc ctgatgggca atcttgcgtt
     1861 gagaagcttt aatatcaggg acaccagtgg ccactacccg gggagaaaga aattgctgtg
     1921 ggatgccgcg aacatgaaga tcacgaattt cgacgaagcc aaccagttcg tcaaacggga
     1981 gtatcggcag ggatggaaat tataatatcg aagggggtcc gacggccccc tttttttatg
     2041 gggccgccta ttattcgttc agcaatttcg ccagctcgag caattctttc tgcttgtagg
     2101 cattccgctc gtcttcgaaa cacttgctgt attcttcgag cgccgtctgg atgatccggt
     2161 aatgcgacag cggtttgaaa taggaagcga ccggcgggac cgatatccgt ttgaaatagc
     2221 tttcgacatc cttatgggag aacacctgtc cgctggtggg gtcgataaag aactcgatcc
     2281 gctgcatggg gctcagcttt tcggggttct cgtcgtaata aggcttgata taggccagca
     2341 cgaactggcg agggatattg atggctttta ccggaatgtc cagcagctcg cacagcaaca
     2401 gatacaggat gccgttggtg atggagttgc cccgtttggt ctcgatcagc ttgttgatca
     2461 ggaattcttc cgggtgcgga taggcgactt ccgtgccttt gagaccatag taattatata
     2521 gaatgctggt gacgatgtgc acctgttcca gcggagtgag atagctgttc agctcgagcc
     2581 agatattgcg ccggagcttt tccacttcat gcaataccgg tgtggtgacc agttcgggat
     2641 attggaattt ggctatcaat agcgacgcgg tgagaaggtc ctggtagctg ctgcttttcc
     2701 agtgttgaaa atcttcggtc agatcgtgat aatgcaattt gtggatcagc agctcgatcc
     2761 gctcctgtac atcgccgctg atcgtgttct cccaaaggtt ctccagattg gggatgatgc
     2821 cgcggccata gctcacaatg cgattgctga cggaatcgaa gacttcctgg tcggggtcat
     2881 cgatcagatg gaaaagcgct aatatttctc tgttctcctg catggtttcc cggtccttcc
     2941 cggctacaaa ggtaaggtcg gggaagttta gtagagttta gtgggtttct tatccacact
     3001 aacgctataa atgttcatta aattgcatgc cagaatgggg cgggccggca caggacgcgc
     3061 cagggccgtg catgcgggtc ccgcagttta gctgcctttt tttctgcgcg gaacccgttt
     3121 cggaggattg gcctttactt cttcaatgat ggcctttact tcatcgaggg aaagatcggc
     3181 ggccgcttcc tgcttttctt tcggcagttt gtaattgcgc agaccctgtt tgatataggg
     3241 accataagga ccgcgcaaca cctgtatctt ttctttttcg aagaccttga tcgtgcgctg
     3301 ctctttggcc gctcgttttt cgacgacgat gggtgtcgcc tggtcgaagg tgatcgtata
     3361 gggatcgaac tctttcccca gcgagtagaa ctttccgtcg tgagcgatat aagggccgaa
     3421 acgaccgata ttcacagtga cttcgttctc ttcgaaaata ccgagattcc gcggcagccg
     3481 gaaaagctcc atcgcctcgt catagctgat cgtttcgata ctttgcgtgt tctttagttt
     3541 ggcgaatcgc ggtttttcct cgtcattgac atccccgatc tgcaccatgg ggccatagcg
     3601 acccatgcgc gcaacgaccc tttttccgct gtcggcgtcg ataccgagct ctctttcacc
     3661 tttgatccgt tcggcattct cgatcgtatt ctcgacgtcc ctcttgaagg ggttgtaaaa
     3721 atcgtcgatc atcttactcc acagcatctt gccctgggcc acctcgtcga actcctcttc
     3781 gatacgtgcg gtgaaatggt agtccatgat gtcgtcgaaa tattgtttga ggaagtccgt
     3841 caccaccagg cccagatcgg tggggaataa tttggacttc tcggatccat aggtctcctg
     3901 ttccgccagt gtttcgatat gttcgttctt caggacgaga atgcggaggt ctctttttaa
     3961 tccctccttg tcccttttct ccacatagcc gcgtttgaga atggtggaga tggtgggtgc
     4021 gtaggtagaa ggccggccga tgcccagttc ttccagcttc ttgaccaggg aggcttcggt
     4081 atagcgtggt tgaggacgcg tgaaacgttc gaccgcacgc atttctttca ggggaagggc
     4141 ctggcccacg cgcaacggcg gcagcatgct ttcctgtgct tcctcttcgc tgccatcgtc
     4201 ttcatccctg tcctccatat atactttgag gaaaccgtcg aatttcagca cttcgccgct
     4261 ggcggtcagg tcttctttat tggtagatat ctggatcttt gcaatggttt tctcggtctc
     4321 cgcatccgcc atctggctgg ccatggtccg tttccaaatg agatcataga gccgccggca
     4381 atcggcatct tcaaccgtca gcgcatctat ataggtggga cggatggctt cgtgggcctc
     4441 ctgtgcggac tcgttcttgt tcttgaactt ccgcgcctgg tggtatttgt cgccgtattg
     4501 actgcggatc tggttagcgg ccgcgtccat ggccagctcg ctgagattga cgctatcggt
     4561 acgcatgtac gttatcttcc cgctttcata cagcttttgc gcgagcagca tcgtcctgct
     4621 gaccgaataa cccagtttgc ggctggcttc ctgctgaagg gtagaagtgg taaacggagc
     4681 ggcaggcgtt ttcttccccg gctttacctg gatatccgtg accgcataac cggcaccgat
     4741 acagccttga aggaattttt gcgcgttgtc ggaggaagat tgttttcggc cctctgcgga
     4801 aaaagtgacg gtctttccgc tgatgtcctt tgcggtaaag agcgcttcta ttttaaagga
     4861 gctgacggcg gtaaaggcat tgatctcacg ttcgcgttcg gcgatgatgc ggaccgccac
     4921 gctttgaacc cggcccgcgc taaggttatt cttcatgctc atcttccgcc agagaaccgg
     4981 gctgagctcg aatcctacga tccggtcgag cacacggcgg gcctgttgcg cgttgacgag
     5041 gttcatatct accgtacggg gccgtttgac cgccgactgg atggcgggtt tggtgatctc
     5101 atgaaaaacg atgcgtttgg tcgtagcagg atcgagaccc agcacttcgc acaaatgcca
     5161 ggaaatggct tccccttcgc ggtcctcgtc agtcgcgagc cagacttcgc ccgatttctt
     5221 ggcgaccgac tttaactctt tgacaacctt ttctttatct tccggaacga tatagcgggg
     5281 tcggtagttg ttcttgatat cgatgcccat gtcatccttc tccagatcgc ggatatgacc
     5341 gtaacagctt tttacctcaa aatcttcgcc cagtatcttc tcaatagtct tggccttcgc
     5401 cggagactcc acaattaata agttcttagc cattttatag tataaagggt taaatcaaag
     5461 cctgattttg tctttgcagg tcttccccca agcttgcaag tttacagtat aacgttcaaa
     5521 ttaaaaaatc agcaggaccg tccggccgcc cggtatggga aaagcgggct ttttttctat
     5581 ttgactatca gttacttatg tactaatctt tatcggaagt tataagttta tgaacaccgg
     5641 aacacgggtc gttgttcaaa atgcgttaat ttgataatca aatgctgtgc tgtggcgggt
     5701 tcgttttcct actccatatt cccacttggc gattcggccg ttaccctgga cattggcgat
     5761 ggcaagggca tcgacgaaca aaacaatatc agggcgctgg ccctgcacga ctggttacag
     5821 acgcatcggt tccccggtat agtggatatc atagtggcat atagttcggt ttcggtgttt
     5881 tatgataccg cggtagtaat aacccaggat cagactgcct tttcgcggat caaggctctc
     5941 ctgacgcagg cctggcagga gaacgatggt gtagtcccgg cgacggttgt cgccgaaggg
     6001 cgtttgatcc ggctgcctgt ctgttatgag gccgcttatg cgcccgatct ggaatgggtg
     6061 gcgcgggaga cgggtctgtc ctgcgaggaa gtgatcaaca ttcattgtgc cgggccctat
     6121 cgggtataca tgataggctt tcttccaggc ttctcttatt taggccgggt agacccgcgt
     6181 ctcgagctgc cccgcaaaaa gtttccggta acggtgcagg cgggcggcgt gggtattgca
     6241 ggcatgcaaa caggggttta tccactgaac agtcccggcg gctggcagat catcggtcgg
     6301 acgccggtgc ggttgttcga tcccgggatg gacccaccgg ttgttttgca gaccggcgac
     6361 cgggtgcaat tctaccccgt tggagacgat gagttccatc ggatcagggc gtccggaata
     6421 gtaagccgtt ttgcataatc agtgatggcc ctgaactttt aaatcagtag cttcggaacc
     6481 tccgaaaaaa acacccaccg gtagcctgtt tgaactgttg ttcaccccac ggagacgctg
     6541 gaagcgtccc ggcacctatt catcaaatcg tttgtcttat ggctttctcc attattaagc
     6601 ccggtttgct cgataccatc caggatatgg gcagacaggg attcggtagc tggggtgtca
     6661 gccctggggg agcgatggat ggctatgcgg cccaggtggc caatctgctg gtgggtaatg
     6721 ataaacatga agcggtgatc gagatccatt ttcccggtcc gcagatcctc tttgaacaaa
     6781 acacgctgat cgcgattacc ggcgccgatt tttccccaac cgtcaacgac gagatcatcc
     6841 cgcgatggca gcccgtcgtc gtgcggaaga atacggtact gcattttcca tcgctggtac
     6901 agggaggaag atgttatctg agcgtgcatg gcggatattg tctgaagaaa tggctgaata
     6961 gttatagcac cagtctgaaa gcaggtgccg ggggatgggg aggacttcca ttaaagaaag
     7021 gggatgaact gcccttcaac gaaagccaga tctattttgc cgggttgctg aaagaggaaa
     7081 acaatttcga ggtattgccc tggcgagtca atatggaaaa gacgtatcaa tttccgcacg
     7141 agatcggttt tctccccggg catgaatggc cgctgttatc cgatgcggcc cggcattcgt
     7201 tcctggaaga taattacatc atccatcctt cttccgacag gatgggttat cagctgagcg
     7261 gtgcgccgat atttcccgat cagcccatcg agctggtttc ttcggccgtg agcttcggta
     7321 cggttcaatt gcttcccagc ggtcagctga tcgtattgat ggccgatcac cagactaccg
     7381 gcggttatcc gcgtgtcgcg cacgtcgtgt cagcgcattt acccaagctc gcacagctgc
     7441 ggccctccga ttcgatacaa ttcaaactga tggataccga ggctgccgaa cagctgctcg
     7501 tctgccgtca gaaagagctg catatcctgc agcggagctg catggatcat ctaaacgaga
     7561 tggtatgctg aggagcccgg gaggggcggg cggggcgcaa tctgaggagc cggccgacgg
     7621 agtacgtttg ccagccatcg atctgaattg tgatatgggc gaagggatga acaccgacgc
     7681 ttctattttc ccttttatta cgtctgcgaa tatcgcctgc ggcggacacg ccggggatga
     7741 cgacaccatg catcggacgg tcgagctcgc tttacagtac ggggtagcga tcggggcgca
     7801 tccttcctat cctgacagac cgaatttcgg ccggatcgat atcctcggca agacactgac
     7861 gttggccgat cttcagggcg tcctgcgtga acagctgcag cggttgcagg acatttgcaa
     7921 ggctgcggga acccgtctcc gtcatgtcaa accacatgga gctctataca atcgcgcggc
     7981 ccgcgatcag cccgtggcgg cgatgatcgc gcaggtggtt gcggatttcg atcactcatt
     8041 gctattgtac ggtctcagcg gaagcgaaat gcgacgttcc gccgatgccg ccggtatccg
     8101 gttcgtcagc gaggtctttg ccgacaggac ataccaaccc gatggcagcc tcaccccgcg
     8161 gtctcaaccc gatgcgctga tcgacgatcc tgcgaaggcc gtagcgcagg tgctgcggat
     8221 gacaaaaaag gggaacgtca tgaccttcct ggggccagtg tccatttcca ccgagaccat
     8281 ctgtcttcat ggtgacggcg cccatgccgt ggaattcgca gggctgatac atcgggcgct
     8341 taccggggag gggatccgga tcgccgcgcc gggcagcagt atactttagc atttcctttg
     8401 caatttccaa aagtttttcc cgttctttgc ctgttcccac tccaacacac gaaaccgcgc
     8461 aacacattgc tgcgcgcaac gcaccatcaa tttgatttta tggcaacgac ggataagacg
     8521 gacaatggcc ggatcgaaag cggctatacc tatgattcat tcaccggggt cggcgaccgt
     8581 gcaaaaaggc cgacggaaaa acatttcctg tggtggtgcg ccggcgccca ccaggatatc
     8641 ctgaaggatt gtccttccga gcaatccaaa tatgcgggtc ttggcggcgt gttgctggcg
     8701 accttcgcgc tggccgccct ggcggcggga tacgcgatct acagcgtatt cagcgacccg
     8761 ttgtgggcgg tatcgtttgc cctcatctgg ggattgatca tcttcaattt cgacaggttc
     8821 ctcgtctcga cgatgcggaa atacggagtc tcgcggcgca aacagatctg gatggccgcg
     8881 ccccggctgg tgctggcact gctcatcggt ttcacaatcg cccggccgct ggagctgaag
     8941 atcttcgaaa aggagatcga cgtccagatc acggccaacc gccatcaaaa aatgttgctg
     9001 aatgacagtc tcctgcaggc cgaacagcga ccgctgctat cgtccgcgtt acaggaacgg
     9061 gagagactga acacgcgcaa gtccggtatc gaagacacgc ttcaccagct gcaacagtcc
     9121 tatatccggg aggctgacgg caccggcggc tcggggcggc gcggcatcga caaacttaca
     9181 cggatgaaac agcaggccta cgaatcgtct ctgacgcagt ttgcgcccga gctcctgcgg
     9241 ctgcagcagc agacgcatta ccaggacagt ctcatcgccg catcgaatgc cgggcgtcag
     9301 cagcgcgatc tccgatatga gacgcaggtg acgaacgacg tgggtttcct ggagaggaat
     9361 aaggcgcttt cggatctgtc ttcgcgtgaa aacagcgttt tctggtccag cctgctgctg
     9421 tccctgctga tcattcttat cgaaatcggg cccgtgttgt ccaagctgat catgaacacg
     9481 gggccgtacg atcttgcgct ggcctccttt gagcttcggc aaatggccac cgcagaagat
     9541 gaaatgcgtc gcgataaaga attgagacac gctaagaagc aggggatata taaagcaaaa
     9601 aaggagatgt cggcggagct attgcaaaaa ttgtccgggt tgcagaaaaa gcatgtacag
     9661 gaggagctgg accggtggga gaagggtgag gagaaagcgg agccgcgggt gtcgctggag
     9721 gaacttgccc ggcggatcag ggaccgctat gattttcgcg aggaggacgt gctgtaggaa
     9781 tccgggaatt ccgccggccg gcgcacaccc tttactcccg gctcgcctca gcattgatat
     9841 agttctccgc cagcgccgtc agcaactcgt cggtctcctt ttcttcatag agcgtcagct
     9901 cgaggaggct ttcgatatca tcttcgtcca tggtccgggc ataggtagcc agacttccat
     9961 aggcgctgat ctcgtaatgc tgcattttct gggcggccac gatcaggccg gcatcccgtg
    10021 tggcggaacc ggccggtgtt gtcgcaatga cctgttcggc ttcgcgggtt attccgaaga
    10081 tggcttcggg gggcagggct tcggcaaccc ttctcagttt cgcgaatacc tggtcgagac
    10141 gatggatatg ttcgcgggta tcttccaggt ggctggacag cacatttcgc aatttcagtg
    10201 agctggcggc ttttttcaac agcggcaata cggtcaactg atgtctttcc gctccatata
    10261 attctcttaa ttcattgaga aagagttcgc cgagtttcga atgccggatg gatgtggttt
    10321 ctttattcat acagctttta ttttaggatg gcggtaaggt tatcctatga tagaattgtg
    10381 ccacgggtgg ccggcgggga atcggggttg tgttaaagct ggcctgcctt tttcgatagc
    10441 atagaaaaag gtatatggaa aagttaaaga ccgccccggt acagatagat ggaggagacg
    10501 gacccgaaga ttacgttgtg caggaaaaag attatggaga tatggtggtc tatgatattt
    10561 tccggaaaga ccactacctg ctgaccatgg ccagggatgg gagcatcctg ttcatgaatt
    10621 tcgatgcaga gcccggggac aaggaagttt tcaagctgtc acggctcaac cagttcatcg
    10681 aaaagataca ggctgcaccg tgagcgttgt ccacaattgt ggaaaaattc tcccctcgtg
    10741 gggaggaatt ttttccccga aatattacag cagttcgtgt ttttttaccc ggtgtaaagt
    10801 gcaaaatgga aatctgcgcg ccagatcgtt caggcgccgt atcgtctctt cattaggctg
    10861 gggcggcggt tcgagcaatt ttatcacgcc agtcctgtgt ttccgggtag ttgaagaaaa
    10921 agcagtcatg gtcgtatgcg acggccaggt cgccaggctt ttagtattca ttagatatta
    10981 ttgtatgggt aactattcaa aaagagtacc accgcctatg acgatttatt atgcttatat
    11041 tggggcgttg atcctattac tcaacacact attcgacccc atctctgatt gaagaaggcg
    11101 ataataatac ggttagcggc ggccctatgc tgcagttggt gttgtctttc ttccttcggg
    11161 caggcgaagg atacgacgag gcacgacagc tcctcgcatt ggtggaacag gaacaatatc
    11221 tttcaattct tccggaatgc catcgtcaag ggcaaacccg acacggcgaa ggacttcaat
    11281 acgctggaac cgctgaatac caagagcgaa acgccttttc acccctacga tggaaagatc
    11341 atccggcata tctatatccg tggttacggg ttcgaacaga cctttcccga cacgtccagg
    11401 cggctcgaat actttgggac gcagatcctt aacgatctcc accggaaaac gcgggattgg
    11461 gtgatccggg acgccctttt cgtcaaggag aataccccgg tcgatgccta taagctggcc
    11521 gataacgaac ggttgatccg tagtctcaac tatatccagg atgcccgttt cctggtgacc
    11581 agccttcccg atagcccgga ttcggtggat ctggtggtcg tggtaaagga tcttttcagt
    11641 atatcgggcg ccatcggcgc gttgggttat ccgccgttta gttcccgggt tacggccagc
    11701 gaggccaact ttctcggtat gggccaacgc attcaggggg gcatgaacct cgagcaggcc
    11761 aggaacccga atttcggggg acagatcctg tatagtaaga cctatatcgg ccactccttc
    11821 accaatgtca cggcttcata ttccgagatc aacccgaata tctataacgg gacgccggat
    11881 gaaacagcct ggctcctcca gctggaccgg cctttgtatg ctccatatgc tcacctggcg
    11941 ggtggcatcc gggtagggga cttccagaat ttcaatgtgt acaaacagcc ggattccctt
    12001 ttttataaat accattatca cacccgtgac gggtggatcg gctggaacat cggttcggat
    12061 cgttttctca gcaataccgt cgtgctcgac cggcgattca tcgccttccg ctatttccgg
    12121 aatgatttcg acagcattcc gaaacaggtg ggtaattcct tcaatttccg gttcaatgac
    12181 agggaggcgg cgctcagttc cttatccttt ttcagacagg agttctataa gaccaattac
    12241 atctatggtt tcggaacgac ggaggacatc cctaaaggat ataatatcgc actgacggga
    12301 ggatggtacc ggcagttgaa tatcaacagg gtgtatgccg gggtggacgc caacggttac
    12361 acggtttcga aacggggcgc ctttacccag ttctttctgc gttcgggggc cttcttttcg
    12421 cacggcagac cgcaggatgc cagcttcctc ataggagcca gctattacag tccgttattc
    12481 gttttagatc gggtaaagat caggcaatat ctcaacttca gctttacccg gctgatcaac
    12541 cgggtggggc tcgattatct gaccatcaac aatgtcttcg ggcttcgtta tttcaccaac
    12601 gattccagtt ccggcgttca gcgcgccacg atgcactcgg agacgacgtt tttcctcaac
    12661 gggaagatcc tgggtttcaa attcgcgcca ttcgctttcg gggacgtatc ctttctgaca
    12721 ccacagagcg ggtcgctgat gaaatcggac ctatgggatg gcatcggcgc gggtatccgg
    12781 acgcgtaatg aaaatctgat ctttaacacc atagagttcc ggatggtgta tttcccgcga
    12841 agggcggagt acaacaattc cttcaagata cttatcaata ccgatatcca gttcaggtat
    12901 aacagcagct acgtacatca gcccgatgtg gtctttctga atacggacgg tttgaacagc
    12961 atttattgac cggcggccgc cgatgatccg atgatggacg cggccagtcg ctgtgcccag
    13021 aaacggtagg tcccgccgtc gggatgaagt ctatccgcga cgaagatggt tgaggtccgg
    13081 ctatgagcgg tattatcgat gaagggcacc tggtgccggc gggtgatcgt ggaggcggtg
    13141 gtgttgaaga catcgatctc ctgtgaaatt cgtccggggt cccggtcacc ggtggctgca
    13201 aagggggaga cgccccaatc cgggatcgac aatacgatta ggcgctctcc gagacccata
    13261 gcacgcgtta gtagcgtttc gaaccctatg ccatattcgt ccgccccgcg gcccctgtat
    13321 tggtcgttga cgccgatcag cagcgttacg aggtcatagc gtgccagcag tcgggtatta
    13381 tccatagcag ctgccagctc gccggtggtc cagccggtgc gggcgatgat ctcgggcgag
    13441 caaaacggcc tgccgccgtt gcgcagcaat tgcacggtct gataggggaa gctgtcggac
    13501 aacggaacgc cttccccgat ggtataggag tcgccgagtg cgagatagga atgcattatt
    13561 gcggccctcc gccaccgccg ccgggcatgc cgccgccttg ctgctgcata ttctgcatat
    13621 tatccatatc gcccttcata tttttgcgtt tgaagagggc catgtcgaat ttgccgaacc
    13681 gccagttgaa ctgcaggcgg aagaattgcg ggtctcttct gcgcgctact tcctgggtgt
    13741 agaaagccgt ttgtgtaaac acatcattga tccgtgtccg gaagatatcg cttacgctca
    13801 tggtaagcga ggccaccttg cccttcagga actcgaaacg caaagcgccg tccactcctc
    13861 ctgacggctt ggaatagccg ttcgcattgc cgctgacgcc tccgccgaaa cctctgccca
    13921 ttccgccgcc gccattgctg gcgctgccgc cggggggcag gaccgtcttg gacgtataat
    13981 cgccggacaa ttggagggtg agggccttat tcggtttgga cattatcttg aagctgttgt
    14041 tgatcttgcc gaaccagctc catatctcgc ctacctgagg aattgcgttt aaaccggtat
    14101 cgatggtatt gatcttcgag gtgaagatgt tgatattgct ggtcaggtcc caccatttcg
    14161 tgatcgtatt acgactgatg atctcgaagc ccccgacaaa gctggacttc gcattgatat
    14221 aggtgctgac atagacgggc tgatgcgaga tcgggttctc tcccggaacg gagatgcggg
    14281 tgatcaggtc cgtggtggat ttatagtagg tcgataacag cagcgtgttg ttgccgggga
    14341 aggacttctg gtaacctaat tcgaacgagt tggtgaactg gggtaccagc gccgggttac
    14401 cccggctggg gttcagggag tcggaatagt cggtaaaggg aaacagctgg aagaaattgg
    14461 gccggtctat ccgacgggta tagttcaact gcaggtcctg gtcatctccc agcttctgcg
    14521 tcaggaagat gctggggaaa aggctgatcg ggaacgtgct gctgaattct cctatcgcgg
    14581 gcagctgatg accgctggtg tcgaacgcgg tataatgctg agttccttta taatccgagc
    14641 tctcgccccg caagccgacc tgataggtaa aatttttgaa gacattgcca tacgttacat
    14701 atcctgcgaa aaccctgtcc tgataagtat attgtgagga caggaggccc gcggtatgga
    14761 agttcccgtc ggtacccatg gtatcgatgt tgttgacgct gttgttggtc cggatggcca
    14821 cgcgcgcgcc ggcctcgaac ttggattttt ccgtcagcgg cagggtatag tcggtctgtg
    14881 ccgtaaaata tttgttattc gaattgccca cctgttcctg ctggtagatg gagcttagcg
    14941 gaccgccctt gacattgtag atattggagg tgatcctatt gagattgtcg ttgctgccct
    15001 ggctgtagtt ggcatcggcc gtccattctt cacccgcctt gggaaaattg tgtttgaagc
    15061 tcagcgaggc tccgtggttg ttgaaatgtg catcggtggt cgagtatctt ccggtatagg
    15121 atgacgtcgt atagccgttg tagagggtgt cgatgttgat atcgctgttg atataagggt
    15181 tgaaggtgcc atgcacgata tttcctgcga tagacagcgt attgcggttg tctatgaaat
    15241 aatcgaggcc gccgcggccg aacgcaaaga agccgtcatt gacgttctta tcgtgttctc
    15301 tcaggctgtc tgcaggctga gggatggtgg aataccgggt ctgatcagtg gtattgatgg
    15361 atctccgctg gccataattg ccggcgagaa agaaattcac cttgccttgt ttgacattga
    15421 tgtcaccgcc caggttgagc tttccgcgct cgtcgactcc cgcacgtacg ttgccgttat
    15481 acccggcctt gcggttcttt ttcaggacga tgttgaggat gcctgcggtg ccgccggagg
    15541 cgtcgtattt cgcgccggga ttggtgatga tctccacgct ctgaatggca tcggcgggta
    15601 tctggtcgag ggtcagcgtc gtgggccgcc catcgacaaa gatggtggga ggggagttgc
    15661 gaagggtgac gttgccgtcg atatccacct gtacggaggg aacgttccgc atgacgtcgg
    15721 tggcagaacc gccggcggca gagatatctt tctccacatt gtagatcttg cggtcgaccc
    15781 ccatggtgat caggggtttc gatgccgtca cggtggcgga ttcgagcatt tgcgcatctt
    15841 cggagagctt gatattgccg agatccttat cgatagcgtt gatcgccgcc gacgcgcccg
    15901 ccatgttctg ctgtccatcg ccattgtcgc cgccaactcc gccgttcatt cctccgggtc
    15961 tcgctcctcc gccacggaga cctttgagat cgaaggccac tttctcttcg taggtcttat
    16021 aaccgatggc cgtgatcttg agacgaaaat tgccgaagag cggaaggttc tcgagactga
    16081 aatcgccttt gtgttcggtg atcatgccgg cgatgacaga atccttttgc gcttttttta
    16141 caggatcgta tttggtgagg accagctgaa cggatgcgcc gtccatgcct ttacccgtct
    16201 tggaatctat cacttttcca tagaaatgac cgatcgacgg aacctgtccg ccaccgcccg
    16261 cgcctcttct accgccaccg ccgcccacag ggggaaagcc ttgtgaaaac ccagccgccg
    16321 acattgccat aaggaggaac aaaagcccaa tttttctcat gagttgtaaa tattcatttt
    16381 cggtgcaaaa ttcagttaaa acttttagca aaaagagcca atctatacaa acggtataat
    16441 atcgtcacgg aagggctaat tttgcgcgtg cttatcggtg gattttcacg cgcccgaccg
    16501 gtgggtttat cgcatcataa aagtgagtgc aatctgtagc aaaccgatca ggaaaccgag
    16561 cgcggcgccg atgatctcca cgaagcgaaa ctccttcgtc aggaattcgt taagaagggc
    16621 ttcgagccgg tgggcagaaa atccggcgat cttttcgacc acgatccgtt cgaggtccag
    16681 atcgtgacga agattggcca cgtaattctt catgatgatt gggaacaatt gttccagctc
    16741 gctcataaat acctccttta gctgaccgat ggtcttctcg ccgatgaaca tcgagatcat
    16801 cggcatggtc tccttcaatt tgatgcggag gaaatggtcg atgtgctctt ccgccagtgg
    16861 caggatgcgc tgcacgttgt cgggattgac gatggcctgt tcgatatcgc cgaaggaaag
    16921 cagctcctgg cctacgatcc gtcccaggct ttcggcgatg agccgttgc
//
LOCUS       DASYSV010000032         6263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2314270, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000032 DASYSV010000000
VERSION     DASYSV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2314270"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VGQ51_00930"
     CDS             <1..1178
                     /locus_tag="VGQ51_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEV9035143.1"
                     /translation="AKMAPGVYRSVMGNQALALGLIAASQKSGLPLFLGSYPITPASD
                     ILHELSRHKSFGVRTFQAEDEIAGITSAIGAAYGGALGVTTTSGPGMALKAEAMGLAV
                     MLEIPLLICDIQRGGPSTGLPTKTEQSDLLQAYYGRNGECPMPVVAASTPSDCFDAVY
                     EAARIALQHMTPVIFLSDGYIANGAEPWKFPKSADLPPITVQFKKGLSEGEEKFQPYL
                     RDENLVRPWAIPGTPGLEHRIGGLEKQNITGNVSYDPENHQQMVKIREEKVARIADNI
                     PQQQLDSGPDKGDLLILGWGSTYGAIKSAAAQLQEEGYSVSHAHLRHLRPFPSNLGDL
                     LRRYRQVLVPEINNGQLIRIIRDQFLIDAKGFQKVMGIPITKTELVMKIREMLGGSN"
     gene            complement(1175..3370)
                     /locus_tag="VGQ51_00935"
     CDS             complement(1175..3370)
                     /locus_tag="VGQ51_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HEV9035144.1"
                     /translation="MDYVRQYKSFINSHYFNGAIRITMGITLPAILLGYFHNLAAGIS
                     ISIGAMCVGNTDNPGPIHHRRNGMIACILIIFLVAWLMGLASPSDVLTGALVFIFCFV
                     FSFIGIYGTRASSIGINALLVMVLNVARPRSGAESFVNAVYVLSGGVWYTLLSLLLYS
                     FRPYKLVQQALGDCMQATAEYLRIKASFYAPHADYEESYHRLLDSQVTVHQKQDLLRE
                     LLFKSRNIVKETTNIGRVLMMIFLDIIDLFERIMTSQQDYQKLHRLFDGTTLMQECRS
                     LILDLAAELDEIGIAVMSGKASNENTSLSERIGAFRTHLERYSSGHRTADNADAFLDL
                     ANILDSIEDLSDRLHTLHGYTTYDRKIIPGFDPGIELDAFVAHQRVGRGLIEDNLTIR
                     SNIFRHSLRVSIATLTAFIISRFLPVGHGYWIMLTAIVILKPAYSLTKKRNFQRLVGT
                     LLGALIGLLLIYFIHNRDVLFVLMIVFMIACYTVLRIDYLVFVILMTPYVLLLFHLLY
                     PVDFRTILTERVIDTLIGSVIAFFANILIIPSWEHEQILDFMTAAINANIAYFRDVAG
                     AFTGRPVSVQRYKLSRKNAFVGLANLSDALSRMLSEPRSRQKNVTDMHQFVVSNHMLT
                     SHVATLAYYAETVRAARADPARYIDAAWQPVVDDIAVRLENTVGLLKNALPVLQRSGS
                     REPWRVLNSRVNAVKELKPIADQFAFIDKVTTDIGKVSSPLHTAFVGAP"
     gene            3481..5946
                     /locus_tag="VGQ51_00940"
     CDS             3481..5946
                     /locus_tag="VGQ51_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9035145.1"
                     /translation="MRTLPLILLTTLSFHILHGQQITGVVQDDQGKPLASASIALEKA
                     IDSSVVKLSISNPNGQYAFAPVTAGRYFVSVTHIGYQPGRSADFETGGQGTFPMSEIR
                     LAPLSGQLTQAVVEAGKPLVEARTDRIILNVEGNINAIGSDALELLRKSPGVTIDKDN
                     NLSLEARGGVQVFVDGRPTYLSGSTLAGYLQTLPSSLIESIEIIPHPSAHYDAAGSAG
                     IINIRLKKNKAYGTNMTVSAGYNIGIYGKYNGSVSLNHRDAHLNVFGDYTYNHSINES
                     FSTMYREQADTAFNQQSTLMTKTGTHSYHAGLDWFADQRSTLGLVVSGSSTIHTVQTA
                     SSTSIVDIPSNSTDRLLVANNHTDETRDNVNADLNYRYADSGGHELNVDMDYGQYHLR
                     GNQLQPNDYYDPTGKQLLYSNVYNIVSPTDIRIYSAKADYATNFAKGRLSLGLKSSFV
                     TTSNIFREDDVYTWGTQRDSLSSNIFDYRENIHAAYADYKRTVRGWDIQGGIRVENTF
                     SEGTSTGYREPEGYILPMGVPHHYVPYDSAFKRNYTDLFPNVSITWNTHPANRWTLSY
                     SRRIDRPAYQDLNPFEFKIDDYTFYRGNTRLLPQYTNSMGLSWLYRHKFSVSLNYSHT
                     SDVFTVLADTTEVSKLIDERENLTSQDVAGMTLSYPFTYKWYSVYASVTRAYIRNKAD
                     FGPGRGIDLGMFNMTAYSQHSFRFGGGWTGQLTEYYSGPTIVQATLRFHSLWSLDAGL
                     QKTILGGNGSFKVSVTDIFHTLNWSATSEFAGQYIRTAGGYESRLLKLYFTYRFGNRQ
                     LKAARRHSNGDEEETKRAGSGSN"
     gene            5977..>6263
                     /locus_tag="VGQ51_00945"
     CDS             5977..>6263
                     /locus_tag="VGQ51_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035146.1"
                     /translation="MQTIIDEIKKLYTDFAGAIAPLTGLEKIPQSGSNRMYFRVLTAT
                     GSCIATYNDNIRENSTFVHFSRHFRAKGCPVPDIYAINDEFTIYLQEDFGDL"
BASE COUNT         1544 a         1738 c         1665 g         1316 t
ORIGIN      
        1 aggcgaagat ggcgcccgga gtgtatcgca gcgtcatggg taatcaggcg ctcgctttag
       61 gactgatcgc cgcctcgcag aaaagcggtc ttccgctctt ccttggctcc tatcccatca
      121 caccggccag cgatatcctt catgagctga gccgtcataa gtcttttggc gtacgcacgt
      181 tccaggcaga ggatgagatc gcggggatca cgtccgccat cggcgccgcc tacggcggcg
      241 ccctgggggt cacgaccacg tcgggacctg gaatggcgct gaaggccgag gccatgggtc
      301 tggccgtcat gctggagatc cctttgctga tctgcgatat ccaacggggt ggtccttcca
      361 ccggtctccc caccaagacc gaacaaagcg atcttctaca agcctattac ggccgcaatg
      421 gcgaatgccc gatgcccgtc gtcgccgcct ctactcccag cgattgtttc gatgccgtct
      481 atgaggccgc acgcatcgcc cttcagcata tgacccccgt catcttcctc agcgacggtt
      541 atatcgccaa tggcgccgaa ccctggaaat tcccaaagag cgcggatctt ccgcctatca
      601 ctgtccaatt caaaaaggga ctcagcgaag gagaagaaaa gttccagccg tacttgcggg
      661 atgagaacct ggtacgcccc tgggctatcc cgggtacgcc gggtctggaa caccgcatcg
      721 gaggtctgga aaaacaaaat atcaccggca atgtcagcta tgatccggag aaccaccagc
      781 agatggtgaa gatccgcgag gaaaaagtgg ctcgcattgc ggataacata cctcaacaac
      841 aactggacag cggtcccgac aaaggcgatc ttctgatact cggctgggga agcacctatg
      901 gcgccatcaa aagcgctgcc gcccagcttc aggaagaggg atattcggtg tcgcatgcgc
      961 atctccgcca tctgcggccc ttcccgtcca acttaggaga cctgcttcgc cgttaccggc
     1021 aggtattggt ccccgagatc aacaatgggc agctgatcag gatcatccgc gatcaattcc
     1081 tgatcgacgc caaaggcttt cagaaggtga tgggcatccc cattaccaag actgaattgg
     1141 tgatgaagat ccgcgagatg ctgggtggaa gtaattaagg cgcaccaacg aatgcggtat
     1201 gcagcggcga actgaccttt ccgatgtctg tcgtcacctt gtcgatgaag gcgaactgat
     1261 cggcaatggg tttcaattcc ttcaccgcat tcacgcggct attcaatacc ctccacggct
     1321 ccctcgaacc cgatcgttgc aggaccggta gcgcattctt cagcagacct acggtatttt
     1381 ccaaacggac ggcaatgtcg tcgaccaccg gttgccatgc cgcgtcgata taccgggccg
     1441 gatcggcccg ggcggccctg acggtttccg cataataagc cagcgtagcc acatgcgaag
     1501 tcagcatgtg attggagacg acgaactggt gcatgtcggt cacatttttc tgtcggctgc
     1561 ggggttcgga caacatccgg gaaagcgcat cggagagatt ggccagacct acaaaagcat
     1621 ttttgcgcga aagtttatac cgttgaacgc tgaccggcct tccggtgaat gcgcccgcta
     1681 cgtccctgaa gtaagcaatg tttgcgttga tggcggccgt catgaaatcg aggatctgct
     1741 cgtgctccca ggatggaatg atgaggatgt tcgcgaaaaa agcgatgacc gacccgatca
     1801 atgtgtcgat aacgcgttcg gtaagtatgg tacggaaatc cacgggatat aaaagatgaa
     1861 acaacaacag cacatagggt gtcatgagga tcacgaacac aagatagtcg atccggagca
     1921 cggtgtaaca ggcgatcata aaaacgatca tcagcacgaa tagtacgtcc cggttatgga
     1981 taaaatagat gagcagaagc cctatcaacg cgcccaacag cgtgccaacc aggcgctgaa
     2041 aattgcgctt tttggtaagg ctataggccg gtttgaggat gacgatcgcg gtgagcatga
     2101 tccaatagcc atgcccgacg ggcaggaacc gggaaatgat aaaggcggtg agagtggcga
     2161 tgctgacccg cagcgaatgc cggaagatat tggaccggat ggtcaggttg tcttcgatga
     2221 gcccacgtcc gacccgctga tgggccacaa aggcatccag ctcgataccc ggatcgaagc
     2281 ccgggatgat cttgcggtca taggtcgtat atccatgcag tgtatgaagc cggtcggaaa
     2341 gatcttcgat actgtcgagg atattggcca gatcgagaaa ggcatccgca ttatccgcgg
     2401 tacgatgccc gctgctatac cgttcgaggt gcgtgcggaa ggccccgatc cgctccgata
     2461 aagaggtatt ttcattggaa gccttgccgc tcatgacggc gataccgatc tcatccagct
     2521 cggccgccag atcgaggatc agcgaccggc actcctgcat gagggtggtc ccgtcgaaga
     2581 gacggtgcag cttttgatag tcctgctgtg aggtcatgat cctttcgaac agatcgataa
     2641 tgtccaggaa gatcatcata agcacccgcc cgatattcgt ggtctccttt acgatattcc
     2701 ggcttttgaa caacagctcc ctgaggagat cctgtttctg atgaacggtc acctggctat
     2761 ccagcagccg gtggtatgac tcctcataat cggcatgcgg ggcatagaag gatgctttta
     2821 tccgcaggta ttccgcagtc gcctgcatgc agtctcccaa ggcctgttgc accagcttgt
     2881 aaggccggaa gctatagagc aggagactga gcaaggtgta ccatactccc ccggacagca
     2941 cgtagaccgc attgacaaag ctttcggcac cgcttctggg tcgcgccaca ttcagcacca
     3001 tgaccaacaa cgcattgatc ccgatcgagc ttgcgcgtgt gccgtaaatg ccgataaagg
     3061 aaaagacaaa acaaaagata aagaccagcg cgcccgtcag cacatccgaa ggggaagcca
     3121 ggcccatcag ccaggctaca agaaaaatga ttaagatgca ggctatcatc ccattacgcc
     3181 ggtgatggat ggggccgggg ttgtccgtat tgcccacgca catcgctccg atggaaatgg
     3241 agatgccggc ggcaaggtta tggaaatagc cgaggagaat ggccggcaga gtaattccca
     3301 tggtgatccg gatggcgccg ttaaagtaat ggctgttaat aaaactctta tattgtcgga
     3361 cataatccat gctgctacca aaatattttg cttcgtcttt gcgccttcaa gaaaaatttt
     3421 ctttccttcg tattataacc cggttgggcc ggccattacg ccgcaaccgc tgccaaaatc
     3481 atgagaacgc tgcctttgat cctattgacc actttatcct tccatatcct gcatgggcag
     3541 cagataacag gcgtcgtgca ggacgatcag ggtaaaccat tagccagcgc ttcgatcgcc
     3601 ctcgaaaagg ccatagattc ctcggtcgtg aaattaagca tttcaaaccc aaacgggcaa
     3661 tacgcgtttg ctcccgtcac cgccggccgt tatttcgtct ccgtcactca catcggttat
     3721 caaccgggga gatcggccga tttcgaaacg ggcgggcagg ggaccttccc gatgtcggag
     3781 atccggttag cgcctttatc agggcagctg acacaggcgg tcgtcgaggc gggaaaaccg
     3841 ctcgtcgagg cgaggaccga ccggatcatc ctcaatgtgg aaggcaacat caatgccatc
     3901 ggttcggacg ctctcgagct gttgcggaaa tcacccggcg tcacgataga caaggacaat
     3961 aatctgagcc tcgaagcgcg gggcggagta caggtattcg tcgacggaag gccaacctat
     4021 ctttcggggt ccactcttgc cggctatctt caaacgctac cctcctcgct gatcgaatcc
     4081 atagagatca tcccgcatcc ctccgcacat tacgatgccg ccgggagcgc cggaatcatc
     4141 aatatccggc tgaagaagaa taaggcatat ggaaccaata tgaccgttag cgccggctat
     4201 aacatcggca tttatggcaa atacaacggc tccgtatccc tgaaccaccg cgatgcacac
     4261 ctgaacgtct tcggcgacta tacctacaac cattcgatca acgaatcgtt ctccacgatg
     4321 taccgcgagc aggccgatac ggctttcaat cagcaatcca ccctgatgac gaagacggga
     4381 acgcatagct accatgcagg actggactgg ttcgcggacc aacgaagtac gctgggtctc
     4441 gtcgtgagcg gatcgtccac cattcatacc gtacagaccg ccagcagcac ttccatcgtc
     4501 gatataccct caaacagcac cgatagactc ctcgtagcga ataaccatac cgacgaaacc
     4561 cgggacaacg tgaatgccga tctcaactac aggtatgccg acagcggcgg ccatgagctc
     4621 aacgtcgata tggactatgg ccagtaccat ctgcgcggca accagctcca acccaacgac
     4681 tattacgacc cgacgggtaa acagctgctc tacagcaatg tctataatat cgtctcacct
     4741 accgatatcc ggatctatag tgcaaaggcg gattatgcca ccaacttcgc gaaggggcgt
     4801 ctctctctgg gtttgaaaag ctcgttcgtg acaacttcca atattttccg ggaggatgac
     4861 gtctacacat ggggcacaca gcgcgacagt ctcagcagca acatcttcga ttaccgggaa
     4921 aacatccatg cagcatatgc cgattacaaa cgcacggtca ggggatggga cattcagggt
     4981 ggtatccgcg tggagaatac cttttccgaa gggacgtcga ccggttaccg ggagccggag
     5041 gggtatatcc tgcctatggg cgtgccgcat cattatgtcc cctatgactc cgcttttaaa
     5101 cggaactata ccgatctttt ccccaatgta tcgatcacct ggaacaccca tcccgccaac
     5161 cgatggacgt tgagctatag cagacggatc gaccggccgg cataccagga tcttaatccg
     5221 tttgaattca agatcgacga ctatacgttt tataggggaa acacgagact gctgccccag
     5281 tacacgaaca gtatggggct ttcctggctg tacaggcata agttttccgt ctcgctgaat
     5341 tacagccata ccagtgatgt gttcacggta ctcgcggaca ccaccgaagt ctcgaagttg
     5401 atcgatgagc gggaaaatct gacgagccag gatgtcgccg gcatgacgct gagctatccg
     5461 tttacttata aatggtatag cgtctacgcg agtgttaccc gagcctatat caggaacaag
     5521 gccgatttcg gtccgggcag gggaatcgat ctgggcatgt tcaatatgac ggcttattcg
     5581 cagcacagtt ttcggttcgg aggcggctgg acggggcagc tgacagaata ttacagcggg
     5641 ccgaccatcg tgcaggcaac acttcgtttc cattcgctgt ggagcctgga tgcgggactt
     5701 caaaagacca tcttaggtgg caacggcagt ttcaaggtct cggtcaccga tatctttcac
     5761 accctgaact ggtcggccac gagcgagttc gcgggacaat atatccggac ggccgggggg
     5821 tatgaaagcc gcctgctcaa attgtatttt acctaccggt tcggcaaccg acagttgaaa
     5881 gccgcgcggc gccacagtaa tggcgatgaa gaggagacca aacgcgcagg gagtggtagc
     5941 aactgatttc ctatcttcgc ggctatcata gccactatgc aaacgatcat cgacgagata
     6001 aaaaagctgt ataccgactt tgccggtgcc atcgcccctt tgacaggatt ggagaagatc
     6061 ccgcaatccg gcagcaatcg catgtatttc cgtgtcctga ccgctacggg ttcctgcatc
     6121 gccacctata acgacaatat ccgcgagaac agtaccttcg ttcatttcag ccggcatttt
     6181 cgcgcaaaag gctgtcccgt accggatatc tatgccatca acgacgaatt cactatctat
     6241 ctccaggaag atttcggaga cct
//
LOCUS       DASYSV010000033         7941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1288397, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000033 DASYSV010000000
VERSION     DASYSV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288397"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VGQ51_00950"
     CDS             complement(<1..284)
                     /locus_tag="VGQ51_00950"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV9035147.1"
                     /translation="MKYNFLLLLMLTTGMGYGQNRHRFSLAGHEFLLDGKPFQIISGE
                     MHPARIPRAYWSQRLRMAKAMGCNTIAVYVFWNYLESSPGHWDIRSGNHD"
     gene            complement(281..1741)
                     /locus_tag="VGQ51_00955"
     CDS             complement(281..1741)
                     /locus_tag="VGQ51_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV9035148.1"
                     /translation="MRNLLFFIALLLVCAGLSAQSSIPILPTDTEMDIIRKAAHVAPS
                     PRQLRWQKLELTAFFHFGINTFTDREWGDGKEDLSLFNPTHLNVRQWVRVAKEAGIRQ
                     VILTAKHHDGFCLWPSKYTDHTIAHTPYKNGHGDIVREFADACHEYGMGMGLYLSPWD
                     RNSPFYGDSAKYNQLFLHWLTELLTHYGKVDEVWFDGANGEGPNGKKQVYAFEAWYHL
                     IRQLQPQAVIAIMGPDVRWVGTETGEGRLTEWSVLPVNARQQESIAANSQKDQTFAPL
                     GNLMDQDLGSREQLMKARGLIWYPAETDVSIRPGWFYHTREDSLVRTPSALMDLYFSS
                     VGRNSVLLLNIPPDRDGLISDADIRSLRGWKRRMDATFGLDLTAGAEGGVFKPNGEVA
                     PRYPGMEVTLDLKGPKSFDVVLLQEDIRQGQRVEQFVFEVEENGQWVQVASGTTIGYK
                     RLLRIPPVTARQVRLRILSSRLDPVIAKIGLYNLAE"
     gene            complement(1746..2435)
                     /gene="rpiA"
                     /locus_tag="VGQ51_00960"
     CDS             complement(1746..2435)
                     /gene="rpiA"
                     /locus_tag="VGQ51_00960"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV9035149.1"
                     /translation="MDNPALAGKKIAAGKAIDYIRNGMTLGLGTGSTAYWAIQGIGQR
                     VKEGLTVKAIATSVQSEALARELDIPLVPFSEIDHLDITIDGADEVDRQLNLIKGGGG
                     ALLREKIVASVTRFYIIIVDETKLVDKLGRFPLPVEVAPFGWELTTRRLSGLGCTPRR
                     RMSADTPFLTDNQHYIIDCSFGPMADPAAVHQQVSAITGVMEDGFFIDMANIVVAGSA
                     DGDARVLTKLS"
     gene            2732..3673
                     /locus_tag="VGQ51_00965"
     CDS             2732..3673
                     /locus_tag="VGQ51_00965"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEV9035150.1"
                     /translation="MATTTASSADIALIFAARALEKEIGHTPLHHITHLFRHPRVQVF
                     AKKEWMQLSGSVKARAAYNIIRKAIEEGRLTRKKTLLDATSGNTGIAYAHIAKRLNIP
                     VALCLPENATRERKEILRSLGVELILTSQMEGTDGAQQVARAMAQEDPSRYFYADQYK
                     NDNNWKAHYYTTAPEIYSALPGITHFIAGLGTTGTFVGTGRRLRELQPSIRLISLQPD
                     AALHGLEGWKHLETALVPGIYDPDVADENEEVSTEEAHALIREAWEKEGLLLSPSSAA
                     NLAGAIRVARRLEEGIIVTVLPDNADKYSEITKKLLA"
     gene            3670..4107
                     /locus_tag="VGQ51_00970"
     CDS             3670..4107
                     /locus_tag="VGQ51_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEV9035151.1"
                     /translation="MIIIEPRVRQLLIEDALNTFPDECCGFMFGREEHDGTRLVVDIL
                     VVDNSKEGDKTRRFEISPQDYMEAERHALLHDWTLLGVYHSHPKHPAIPSEHDRKAAQ
                     PYFSYVIISVIDEGEKKVTHLRSWLLSDESQFEEEQVLDYQYS"
     gene            4119..5540
                     /gene="moeB"
                     /locus_tag="VGQ51_00975"
     CDS             4119..5540
                     /gene="moeB"
                     /locus_tag="VGQ51_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEV9035152.1"
                     /translation="MATVIIPTPLRKFTNNAARLQVKPGTIQDTVEELTSNFPDLKKH
                     LLDDQGRIRSYVNIFIGNDDIRDLQQEKSAVKEGEVISIIPAIAGGSPDFTKEELARY
                     NRHIIIPGFGMDAQLKLKAAKVLVIGSGGLGSPVLLYLAAAGVGTIGIVDFDVVDDSN
                     LQRQVLFGVDAIGQPKVEAARRRLTALNPHIRINLYNTHLNSQNALDILKDYDVIADG
                     TDNFPTRYLVNDACVLLGKPNVYASIFQFEGQVSVFNYRDAAGKPGPNYRDLYPTPPP
                     PGLVPSCAEGGVLGVLPGIIGSLQALEVIKVITGVGEPLSGRFYIFDALGFESRTFTI
                     RPREDNPLNGKNPTITTLIDYEQFCGMRAVEERPLKEVTVKELYDLQVKGESFQLIDV
                     REPHEYEIVNIGGELIPLASVAANNDRIDRERKVVVHCKVGGRSAKAIRELEEKFGFT
                     NLYNLKGGILAYIDEVQPELTKY"
     gene            complement(5577..7622)
                     /locus_tag="VGQ51_00980"
     CDS             complement(5577..7622)
                     /locus_tag="VGQ51_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV9035153.1"
                     /translation="MITQEILLKAWRLMYTAATMASTYESNRSLCKYVHSTSRGHEAI
                     QLATAFHLHPQDWVSPYYRDESLLLGLGFEPYQLMLQLLAKADDPFTGGREYYSHPNY
                     RGDDRPRMIHQSSATGMQVIPATGIAQGISYLQSIGRGNGAPIVVCSLGDASITEGEV
                     SEAFQFAVLKKLPILYLVQDNGWGISVRSDEARAMDAFEYAAGFKGMGRMRIDGSDFI
                     TSYERMGEVIGAVRDGRQPWLVQATTPLLGHHTSGVRKETYRDAEDMDTHSASDPLPK
                     LRTRLLALGVEEEQLIQIGNEARETVARQFADAVAAPEPDPSTVADFVFIPTPVTQEK
                     GIREPEGGSKVIMVDAALFAIREIMEDHPEALLYGQDVGRRLGGVFREAATLAERFGD
                     HRVFNTAIQEAYIVGSTVGMSAVGVKPIVEIQFADYIYPGFNQLVTEISKSCYLSRGK
                     FPVQTVIRIPVGAYGGGGPYHSGSIETTLLSIKGIKVAYPSNAADMKGLMKAAFLDPN
                     PVVMLEHKGLYWSKVPGTEEAKTIEPSRDYILPLGKGRVVLAANEDLIAAGDTCCIVT
                     YGMGVYWAKSTAPRFRGRIGIVDLRTLFPLDEELVFATVRRHGKCLVLTEEQQNNSFA
                     EALGWRITRQCFTSLDAPVEVLGALDLPAVPMNIGLENAMLPNPDKIARVIERLLGY"
     gene            complement(7653..>7941)
                     /locus_tag="VGQ51_00985"
     CDS             complement(7653..>7941)
                     /locus_tag="VGQ51_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV9035154.1"
                     /translation="DNSAAIGKWIPLQNIKNKKDINFCMYKNKELVQQGNSGLMINGF
                     DKVVAYISNYFSVNIGDLVFTGTPAGVGECVVGDELEGFIEDESLFTVEIK"
BASE COUNT         1869 a         2317 c         2009 g         1746 t
ORIGIN      
        1 atatcatgat tgcccgatcg aatatcccaa tgtcccgggg aagattccag ataattccaa
       61 aacacataaa cggcaatggt attgcaaccc atcgcctttg ccatcctgag gcgctgcgac
      121 cagtacgcgc gcggtatgcg ggcaggatgc atctcgccac tgatgatctg aaagggtttt
      181 ccatccagta agaattcgtg cccggcaagt gaaaaccgat gccggttctg cccgtatccc
      241 atgccggtag tcagcatcag cagtaaaaga aaattatatt tcattcagcc agattgtata
      301 accctatttt tgcgatcacc ggatccaacc gcgatgagag aatgcgcaga cgcacctgcc
      361 gggccgtcac cggcgggatc cgaagcaaac gtttgtaccc gatagtcgtc ccggatgcga
      421 cctgaaccca ttgcccattc tcctccactt cgaaaacaaa ttgttctacg cgctgcccct
      481 gacggatgtc ctcctgtaaa agcaccacgt caaatgattt agggcctttc agatccaacg
      541 tgacttccat tcccggatac cgcggcgcca cctcgccgtt gggcttaaat actcccccct
      601 ccgcacctgc cgtcagatcg agtccaaatg tcgcatccat ccgtcgcttc catccccgca
      661 ggctccggat atccgcatcg ctgatcaacc catcccgatc cgggggaata ttcagcaaaa
      721 ggacactatt cctccccaca ctcgaaaaat aaaggtccat caaagctgaa ggcgttcgca
      781 ccagcgaatc ttcgcgtgta tgataaaacc aacccgggcg tatgctcaca tccgtctccg
      841 ccggatacca gatcagcccc ctggccttca tcaattgctc cctgctaccc agatcctgat
      901 ccatcaggtt tcccaacggg gcaaacgtct ggtccttctg tgaattggct gcaatgctct
      961 cctgttggcg cgcattcacg ggcaacacgc tccattccgt cagccgcccc tcacctgtct
     1021 ccgtgcccac ccaccggaca tccgggccca tgatcgcgat cactgcctgc ggctgcagct
     1081 gacggatcag atgataccat gcctcaaaag cgtacacctg tttcttccca ttgggccctt
     1141 ccccgttggc accatcgaac cacacctcat cgaccttgcc ataatgcgtc agtaattctg
     1201 tcagccagtg caggaacagc tgattgtatt tggcagaatc cccataaaaa ggagagttgc
     1261 gatcccaggg cgaaagatat agtcccattc ccatcccgta ttcatggcag gcatccgcaa
     1321 attctctcac aatgtcccca tgcccgtttt tatatggcgt atgtgcgatc gtatgatccg
     1381 tatacttact gggccacaga cagaacccat cgtggtgttt agccgtcagt atcacctggc
     1441 ggatcccagc ctcctttgca acccgcaccc actgccgcac attcaaatgc gtggggttga
     1501 acagcgaaag gtcttccttt ccatctcccc attcccgatc agtgaatgta ttgatcccga
     1561 aatggaaaaa cgccgtcagc tcaagcttct gccagcgcag ctgccgcggc gacggcgcca
     1621 catgggcggc tttccggatg atatccatct ctgtgtcggt gggaagaatg ggtatagagg
     1681 actgagcgga caatcctgcg cataccagca acagggcgat aaaaaaaagt agattacgca
     1741 ttttttcagc tgagtttggt aagaacgcgg gcatcgccgt ccgccgatcc ggcgacgact
     1801 atattagcca tatcgataaa aaaaccgtcc tccatgacac ccgttatcgc gctcacctgt
     1861 tgatggacgg cggccggatc ggccatcggc ccgaaagaac aatcaatgat ataatgttgg
     1921 ttgtcggtca gaaaaggagt atcggccgac attcgccgac gtggcgtaca acccagcccg
     1981 ctgagccggc gcgtggtcag ctcccagccg aatggggcta cttcaacagg aaggggaaag
     2041 cggccgagct tatctacgag tttggtctca tctacgatga tgatgtaaaa tcgggtgacc
     2101 gaggcaacga tcttttcccg taacaacgct ccgccgccgc ctttgatcag gttcagctgt
     2161 ctgtcgacct catccgcgcc gtcgatggta atatcgagat gatctatctc ggaaaaaggg
     2221 acgaggggaa tgtccagctc acgtgcgaga gcctccgatt gcacggaagt cgctatggct
     2281 tttaccgtca atccttcttt gacgcgttgg ccgatgccct ggatcgccca gtaggccgtg
     2341 gagcctgtcc cgagtccgag ggtcatgccg ttacgaatat aatctatagc ctttccggct
     2401 gcaatctttt taccagcgag ggcgggatta tccattcctt tacgttaaaa ttgaaccaaa
     2461 aatacaaatt acctgttaac aggtaggaaa tacgcaaggt ttatcctact tttggcccat
     2521 cattcatcga aatgaccaag accactctcc atacgctagc atctcgcacc gaagccacag
     2581 gtttcgaggc gggggtttgt tgttgttgct gttgtcggca agccacggtt attagggtgg
     2641 aggataacct cttataagct aaacgaactt attataaata aatacgaagc tccacctcag
     2701 tggggctttt tttattttat ctatattgaa tatggcaaca acgaccgcct catccgccga
     2761 tatcgcgctt attttcgccg ccagggccct tgaaaaagag atagggcata cgccgctgca
     2821 tcacatcacc catctgttcc ggcatccccg ggtgcaggtg tttgccaaga aggaatggat
     2881 gcagctgtcg ggtagcgtga aagcgcgcgc cgcttacaat atcatccgga aggcgatcga
     2941 agagggcagg ctcacccgca aaaaaacgct cctggacgcc accagcggca atacgggcat
     3001 tgcctacgct catatagcca aacgcctgaa tatcccagta gcgctttgtc ttccggagaa
     3061 cgccacccgc gagcggaagg agatactccg gtcgctgggg gtcgagctga tcctgacctc
     3121 gcaaatggaa ggcacagacg gggcccagca ggtagccagg gcgatggcgc aggaagaccc
     3181 ttcccgatac ttctatgccg accagtacaa aaatgacaat aattggaagg cccactatta
     3241 tactacggcc ccggagatct attccgcgct gcccgggatc acccatttca ttgcaggatt
     3301 gggcaccacc ggtacctttg tcggcaccgg ccggcgactg cgcgagctac agccatccat
     3361 ccggctcatc agccttcagc cggatgccgc gctgcatggc ctggagggct ggaaacatct
     3421 ggaaacggcg cttgtgccgg gtatctacga tccggatgtg gcggatgaga atgaagaggt
     3481 gagcaccgaa gaggcgcatg cccttatccg cgaggcatgg gaaaaagagg gattgctgct
     3541 gagcccgagc tccgccgcca atcttgcagg cgcgatccgg gtcgcacgac gtctcgaaga
     3601 aggaattatt gtcaccgtcc tacccgacaa tgcagacaaa tacagcgaga tcactaaaaa
     3661 attgctagca tgatcatcat cgaaccaagg gtcagacaac tgctgatcga agacgcgctg
     3721 aacaccttcc ccgacgaatg ttgcgggttt atgttcggca gagaggagca tgacgggaca
     3781 agactcgtag tcgacatcct cgtcgtggac aactcgaagg aaggggacaa gacaagacgt
     3841 ttcgagatct ccccgcagga ttatatggaa gctgagcgtc atgcattgtt gcacgactgg
     3901 actttgctgg gtgtttatca ttcacatccc aaacatccgg ctattccttc ggaacacgac
     3961 cggaaggcgg cgcaacccta tttttcctat gtcatcattt ccgtgatcga tgaaggggag
     4021 aaaaaagtaa cccatttgcg ctcgtggttg ttgagtgatg aatcccagtt tgaagaagag
     4081 caggtactcg attatcaata ctcataaaag catacactat ggcaacggtc attatcccca
     4141 cgcctcttcg gaaatttacc aacaacgcgg ccagactaca ggtcaaacct ggaactatac
     4201 aggataccgt cgaggaactg acgagcaatt ttccggatct gaaaaaacat ctcctggacg
     4261 accagggcag gatccgcagt tatgtgaaca tattcatcgg caatgacgat atccgcgatc
     4321 tccagcagga gaagtcggcg gttaaggaag gcgaagtcat cagcatcatt cccgccattg
     4381 ccggcggctc gcccgatttt acaaaagaag aactcgcgcg ttacaaccga cacatcatca
     4441 tcccgggttt cggtatggat gcacagctta aactcaaagc ggccaaggtc ctcgtcatcg
     4501 gctccggtgg tctcggcagc ccggtcctcc tctatcttgc cgccgcaggt gtaggaacca
     4561 ttggcattgt cgatttcgac gttgtcgacg acagcaatct gcagcgccag gtgcttttcg
     4621 gagtcgatgc catcggccaa cccaaagtgg aggctgcgcg ccggcggtta accgcgctga
     4681 atccccatat ccggatcaat ctctataata cccatctcaa ttcccagaat gcgctggata
     4741 tcttaaaaga ctatgacgtc atcgccgacg gtacggataa cttcccgacc cgctatctcg
     4801 tcaacgacgc atgcgtgctc ctcggaaaac ccaatgtcta cgcctccatc ttccaattcg
     4861 aggggcaggt gtccgtattc aattaccgcg atgccgccgg caagcccggc cccaactacc
     4921 gcgacctcta tcctaccccg ccgccccccg gtcttgtacc cagctgtgcc gaaggaggcg
     4981 tcctcggagt actccccggt atcatcggca gcctgcaggc gctggaagtc atcaaggtca
     5041 tcacgggagt aggcgagccg ctttcggggc gtttctatat cttcgatgcg cttggtttcg
     5101 aaagccgcac cttcaccatc cggccgagag aagacaaccc gctcaacggt aagaacccca
     5161 cgatcacgac actcatcgat tacgaacagt tctgcggaat gcgcgcggtg gaagaacggc
     5221 cgctcaaaga ggtgacggta aaagagctat atgatctgca ggtaaaaggc gagtccttcc
     5281 agttgattga cgttcgcgag ccgcacgaat acgagatcgt caacatcggc ggcgagctga
     5341 taccgctggc ctcggttgcc gccaataacg accgcatcga cagggaaaga aaagtggtcg
     5401 tacattgcaa agtaggcggt cgcagcgcca aagccattcg cgagttggag gaaaaattcg
     5461 gttttaccaa cttgtataac ctgaaaggag gcatcctggc ctatatcgac gaagtacaac
     5521 cggagctgac gaaatactaa gcttgtgccg cgacggctcc tccgggcccg ggaagctcaa
     5581 taccccagca acctttcgat cacgcgtgca atcttgtcgg gattgggtaa catggcattt
     5641 tccagcccta tattcatagg caccgccggc agatccaatg cgcctaacac ttcgaccggt
     5701 gcatccagcg aggtaaaaca ctgacgcgtg atccgccatc ccagcgcttc cgcaaacgaa
     5761 ttattctgtt gctcctccgt caggaccagg cacttcccat gccggcgcac cgttgcgaag
     5821 accagttctt cgtccagggg aaaaagggtc cgcagatcga cgatcccgat ccggccgcgg
     5881 aaccgggggg ccgtcgactt cgcccagtat acccccattc cataagtgac gatacagcag
     5941 gtgtcgccag cagcgatgag gtcttcattg gcagccagca ccacccttcc tttacccagc
     6001 ggcaggatat agtcccgtga aggttcgatg gtcttcgcct cttccgtgcc tggcacctta
     6061 ctccagtaaa gccctttgtg ttccagcatg accacagggt tcggatcgag gaaggccgcc
     6121 ttcatcaacc ccttcatatc ggccgcattg gacggatacg ccactttgat gcctttgatg
     6181 gatagcaaag tcgtttcgat gcttccgcta tgatatggtc ctcccccgcc atacgctccg
     6241 acagggatcc ggatcaccgt ctgaacgggg aatttacccc tgctcagata gcaggacttg
     6301 gaaatctccg tcaccaattg gttaaagccg ggatagatat aatcagcgaa ttgtatttcg
     6361 acgatgggct tgacgccgac ggcgctcatc cccacggtgg atccgacaat atacgcttcc
     6421 tgtatcgcgg tattgaatac ccggtgatcg ccgaaccgct cggcaagggt ggccgcctcg
     6481 cgaaatacgc ctccgagcct tctgccgacg tcctgtccgt aaagaagggc ttcgggatgg
     6541 tcctccatga tctcgcggat cgcgaaaagg gccgcgtcga ccatgatcac cttcgatccg
     6601 ccttccggtt cgcgtatacc tttttcttgc gtaacgggtg tcggtatgaa tacgaaatcc
     6661 gcgaccgtgg aagggtccgg ctcgggtgcg gctaccgcgt cggcaaactg acgggccacc
     6721 gtctcccggg cttcgttccc gatctgtata agctgttctt cttcgacgcc cagcgccagc
     6781 agccgcgttc ttagtttcgg aagggggtcg ctggccgaat gcgtgtccat atcttcggca
     6841 tcgcggtagg tttcctttcg gaccccgctc gtatgatgac ccagcaaggg ggtagtagcc
     6901 tgaacaagcc agggttgacg gccgtcgcgg acggctccga tcacttctcc cattctttca
     6961 taactggtaa tgaagtcgct gccatcgatg cgcatccttc ccattccctt aaaacccgcc
     7021 gcatattcaa acgcgtccat ggcacgcgct tcgtccgacc ggacactaat gccccatccg
     7081 ttgtcctgta cgaggtagag gatcggtaat tttttcagta cggcaaactg aaaggcctcg
     7141 ctgacctcac cttccgtgat gctggcgtcg ccaagcgaac aaaccacgat cggtgctccg
     7201 ttacctctcc caatggattg taaataagag atcccctgcg cgatgccggt agccggaata
     7261 acctgcatac ccgtagcgct gctctgatgg atcatccggg ggcggtcatc accccggtaa
     7321 ttcggatggg aatagtattc gcggccgccg gtaaaagggt catcggcctt tgcaagcagc
     7381 tgcaacatca actgataagg ctcgaacccg agccccaaca aaagactctc gtcccggtag
     7441 taagggctca cccagtcctg tggatgaaga tgaaaggccg tggccagttg aatcgcctca
     7501 tgcccgcggg aagtagaatg gacgtatttg cagagcgaac ggttgctttc gtatgtggaa
     7561 gccattgtcg cggcagtata catcagccgc caggctttta gcaagatttc ttgggtgatc
     7621 atgggatttg gaagcccgct tcccgtcgcg ggctatttta tttcgaccgt gaataagctc
     7681 tcgtcctcga taaagccttc cagctcatct ccgacaacgc attctccgac acccgccggg
     7741 gtccccgtga aaaccaggtc cccgatattg acggaaaaat aattggagat ataagcgacc
     7801 actttgtcga aaccattgat cattagaccg gaattcccct gttgtaccag ttccttattc
     7861 ttgtacatgc agaagttgat atccttttta ttcttgatat tctgcaacgg tatccatttc
     7921 ccgatggcgg cggaattatc c
//
LOCUS       DASYSV010000034         8558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1161526, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000034 DASYSV010000000
VERSION     DASYSV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161526"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VGQ51_00990"
     CDS             <1..709
                     /locus_tag="VGQ51_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV9035155.1"
                     /translation="LVEQLSKQNTQLMDFCSIVSHNLRAPLVNMSMLVDFIEASQDPD
                     EQRMLISKLRPVIGNLHATFNELVESIQIRQDLEVKKENVCLHECIKRTVSGLETEIN
                     KLGAKIETDFSEVAVVHYPPAYMNSIIHNLISNSLKYHYPGRSPLIRVRTRLTADKIV
                     LSVQDNGLGIDLEKHRDNFFKIGKVFHRHPNAKGFGLYMTKTHVEAMGGRIWVESVPD
                     EGSTFFIEFENRIKQAS"
     gene            706..1110
                     /locus_tag="VGQ51_00995"
     CDS             706..1110
                     /locus_tag="VGQ51_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9035156.1"
                     /translation="MKSIFLIDDDPIFVYLTRRIIASIDRQCKINEFTDGELAIRYLG
                     EICDKPELLPDIIFMDISMPVMDGWEFLNEYSQLRPKIKKDIALFIVSSSISPQEVER
                     SKGYSAVSDFLIKPLERGKIVEIIDNAKETAE"
     gene            1202..2368
                     /locus_tag="VGQ51_01000"
     CDS             1202..2368
                     /locus_tag="VGQ51_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV9035157.1"
                     /translation="MFDPNLVDLTVLRKRAYNLRWATVPEGVIPLTAADPDTPCAPEI
                     AGAIARFAQDRYFCYGPPDGLPEFRESLACYYQTKRRIPASPSWIFPVDSAAFGIYLT
                     CKAFLSPGDEAILFDPVDFLFGYSIGAAGAKPVPYALPAGEELDIRRMEALITPRTKL
                     ICLCNPLNPTGKVFTEKELLLIGGLACKHDLLILSDEIWSDIVFSPHVYTSIASLNDD
                     IRNRTVTITGFSKSYGLAGLRIGAVMASDRARYQRLFDASLHNTTIHGSNLIGQVAAT
                     TAICACEGWLKEFVRHLQEMRDLCVHRLNAIDGFSCVPPEGCYVAFADISGTRLTSRE
                     MQELLLTKARVAVVPGLPEWFGDGADGYIRLSFATTREILTDALDRIKNTIQQL"
     gene            2365..4311
                     /locus_tag="VGQ51_01005"
     CDS             2365..4311
                     /locus_tag="VGQ51_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9035158.1"
                     /translation="MKIVIIGGGIAGMTIGIELQKKGIEVTIAERQPDLNEKGHAFLM
                     HDDALSILRELGGDGTDLPGQSVGRFCLRRPNGYEVKNVELDSWRCMKRCELINFLRG
                     LLKPDTIVRGRAFSHFIRKDGKAVAAVFANGDVEYGDIFIGADGGNSKVREALFGKVA
                     LTAVAVKEIVGISHNREIAARHTNTFLKFQEIDRGLAFGMIPTSENEFVWFMQYDPTI
                     GDAPEDRPEKIRAFCNRMLRGFPEVTAEILRSTDFSKTYIWSTRDFDLLPAFHRQNVV
                     LLGDAAHLTLPFTSAGTTNAIVGSKILLQCLEDTENHEAAFRDYYRQRGPVIEKHILL
                     GRQLKKIFLFPSGREESPLPFLASSRNAEGEKVTMIKRVIRSGPREKLLRVKYFTDPI
                     CSTCWIIQPIWKKLSLEYGHYLDIQYVMGGLLPSWKDCKGKIISPSDAAIHWKEVSDS
                     YKMPIDSDVWIEDPLSSSWPPSIAFKAAQLQDEPRALLFLRRIREMIFLEKKNIMKWS
                     FLEMAAVEVGLDVPRFRSDYVNEARASFDKDLELARVMNVNSFPTLFFSDQAGNEVRL
                     KGYQHYQDFERVILQLLPFAKRAEIDRQPESLFRSFPTMVDAEFALLSNLMRKDARQV
                     LLQLNQQGFIEKVESGNGILWKMK"
     gene            complement(4397..5740)
                     /locus_tag="VGQ51_01010"
     CDS             complement(4397..5740)
                     /locus_tag="VGQ51_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEV9035159.1"
                     /translation="MKRLTGLLLALALSQQLFSQQVIPDSNSHWSVHFQMTVIDQIHT
                     SFHTAYSGDNSFADTVEPGAVSVTSTLFIGRKLWPGAAFYFNPELSGGRGLSSALGIA
                     GALNGETYRIGDPSPVVSIARGYLQQMIPLGSSDSVSFDNGKNQVAVRVPANRLVITA
                     GKFSMSDFYDNNTYSHDPRTQFLNWSLMSNGAWDYPANTKGYTMGAMVEWIEPNWALR
                     VSSVAVPVIANHSEMEYKFGKAHSETAEFQHNLTFGKRSGVVRLLFSYTASRAPSYQQ
                     GLAALRDGDTGLLQVISGDGKATAYGGKKTGIGLNLEQELTGDISLFARLGWNDGKYV
                     SWAFTEIDQTVHAGLNFKGTQWKRKEDVAGIAGVINGISSDHRAFLKAGGYGFIIGDG
                     ALNYGNEAILEAYYNAKLFDHVWLSVDYEFVHNPGYNKDRSGPVHVFGVRGHVEF"
     gene            complement(5815..6438)
                     /locus_tag="VGQ51_01015"
     CDS             complement(5815..6438)
                     /locus_tag="VGQ51_01015"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV9035160.1"
                     /translation="MIPIFAVQAGIKSSFVRTDNNAVYTLYDETLKTWEPDTSVSNHF
                     IYKENINAAYVDWHQQMGKFAKTNDKNTFGISYGIRIERPSYQSLNPFRFEDELLTFK
                     EHGDSNYTIIQTSGNLASQRNIGLGVNYSKQLMRSWTFNASVNVYNNRYRGVVDSTPG
                     ATKRKRRRRRAKPGRRPQRSAISRAAIPQRQRCNHPCRHPPTATTLQ"
     gene            complement(6481..7161)
                     /locus_tag="VGQ51_01020"
     CDS             complement(6481..7161)
                     /locus_tag="VGQ51_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEV9035161.1"
                     /translation="MIKAPVITFFLLLMTPSIIGGGFMMEKNVSFSAIDALHLNKHDR
                     KFVQTYLLENRMIFSAIRKHDGSRFIITDAVFNQFELPLQLKYLAVIESDLKSDAVSR
                     VGAVGPWQLMPETARLLGLKVTGDRDERTQFDKSTRAAARYLKDLYAEYGDWLLVLAA
                     YNGGEGPVNHAIRKSGSRNFWLLQNYLPSESRGHVKKFLATLLYFEGPSQAASFTGTA
                     APYPVRSV"
     gene            complement(7267..>8558)
                     /locus_tag="VGQ51_01025"
     CDS             complement(7267..>8558)
                     /locus_tag="VGQ51_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEV9035162.1"
                     /translation="GTTPEMIDYFSRILHYDFPWNKYAQIVVRDYASGGMENTTATLL
                     NEYVQQTPRQLLDAYYSKGQSTIVHELFHQWFGALVTCRSWSNQAVDETFAEFSDGLW
                     AEHQYGRDAGDALRAHDVQIYLKTKSVDTAPMVRYHYRNELDMFDVLTYYKGNGVLNM
                     LRNYLGDSAFFSGLSHYLHVNAFKNAEVDQLRLALEDVSGQDLHWFFDQWYYRAGHPI
                     LDIRHRWNDTTATETVWLRQTQEGGPYLLPMAIDIYSGGRKQRYMVRMENKLDSFSFH
                     ASSKPDLVNVDADKVLVAVITDHQSLDEYVFQYFHAPLYLDRSQAINAAIEDPSDARS
                     QEILIAALNDPYYGLRINAVSALDMKNPVIRNAASPILVQLIHSDSNHLVKAAALSVL
                     GKLRDREYMPVFKEALTSPSDAVRSAAGTAVGLLDRN"
BASE COUNT         1938 a         2210 c         2428 g         1982 t
ORIGIN      
        1 gctggtcgaa cagctttcca aacagaatac ccaactgatg gatttctgca gtatcgtctc
       61 gcataacctt cgcgcgccgc tggtcaatat gtccatgttg gtggatttca tcgaggcgag
      121 ccaggacccg gacgaacaac ggatgctgat ctccaagtta cgtcccgtga tcgggaatct
      181 tcacgccacc tttaacgagc tggtagaatc gatccagatc aggcaggacc tggaggttaa
      241 aaaggagaat gtctgtctgc acgaatgcat caagcggacc gttagcgggc tggagaccga
      301 gatcaacaaa ttaggtgcta agatagagac cgatttcagc gaggtggcgg tggtgcatta
      361 tccgccggcc tatatgaata gcatcattca caatctgatc agcaattcgc tgaaatacca
      421 ttatcccggc cggtcgccgc tgatccgtgt caggacgcgg ttgacggcgg ataagatcgt
      481 gctttcggtg caggataacg gcctggggat cgacctggaa aaacaccgcg acaatttttt
      541 caagatcgga aaggtgtttc accgccatcc caatgcaaag ggcttcggcc tctatatgac
      601 caaaacgcat gtagaggcca tggggggaag gatctgggtg gagagcgttc cggacgaagg
      661 gtccaccttt ttcattgaat tcgagaatcg aataaagcaa gcttcgtgaa gagcatattc
      721 ctcatagacg atgaccccat cttcgtatat ctgacgaggc ggatcattgc ttcgatagac
      781 cgccagtgta agatcaacga gtttacggac ggtgagctgg cgatccgtta tctcggggaa
      841 atatgcgaca aacccgaatt gctgccggac attatattca tggatatcag catgcccgta
      901 atggacggct gggaattcct gaatgaatat tcgcagctga gaccgaagat aaaaaaggat
      961 atcgcgttat ttatagtttc atcctctatc tcgccgcagg aagtcgaacg ctcgaagggc
     1021 tatagcgccg tcagcgattt tctgatcaaa ccgctggaaa gaggcaagat cgtggagatt
     1081 atcgataatg cgaaggaaac tgcggagtga ccgagcgtag cgaaggtcac gaacgagcgc
     1141 ttgccggtgg caagggcgaa gttccccgcg ggttccgaca acctgaccca aaccctatcg
     1201 tatgttcgat ccgaaccttg tggaccttac cgtcctgcga aagcgcgctt acaatctccg
     1261 gtgggcgacc gtcccggaag gggtcattcc cctgaccgcg gcggaccctg atacgccctg
     1321 cgcgcccgag attgccgggg ccatcgccag atttgcccag gaccggtatt tctgttatgg
     1381 tccaccggac ggactacccg agttcaggga aagcctcgcc tgctattatc aaactaaaag
     1441 acggataccg gcttcaccct cgtggatctt ccccgtggat agcgccgcat ttggcatcta
     1501 tctcacatgc aaggcctttt tatcacccgg ggatgaggcc attctcttcg atcccgtcga
     1561 tttcctgttc ggctactcca tcggggccgc cggagccaaa ccggtgcctt acgcattgcc
     1621 tgcgggagag gaactggata tccggcggat ggaagcattg atcacgccac gaacgaagct
     1681 catttgtctg tgcaacccgc tgaacccgac cggcaaggtc tttaccgaaa aggagctgct
     1741 gctcatcggc gggctggcct gcaaacacga cctcctgata ttgtccgacg agatctggag
     1801 cgatatcgtc ttttcgccac atgtgtatac cagtatcgcc tcgctgaatg acgacatccg
     1861 gaacaggacg gtgacgatca ccgggttcag caaatcctat ggtctggccg ggttgcgcat
     1921 cggcgccgtc atggcttccg atcgcgcccg ctatcaacga ctcttcgacg cctctctgca
     1981 taacaccacc atacacggaa gcaacctcat cggacaggtg gccgccacca cggcaatttg
     2041 tgcatgtgaa ggatggttga aggaattcgt gcgacatctt caggaaatgc gtgatctctg
     2101 cgtgcatcgc ctcaacgcga tcgacggctt cagctgtgtg ccgcccgaag gttgctatgt
     2161 ggcgtttgcc gacatatcag ggacgcgttt gacgtcacgt gagatgcagg aattactgct
     2221 gacaaaagcc agggtcgccg tcgttcccgg actgcccgag tggtttggcg atggcgccga
     2281 cggttatatc cgtctgagct ttgccacgac acgggaaata ttaaccgatg cacttgaccg
     2341 tatcaaaaac accatacaac aattatgaaa atcgtcatca ttggaggggg tatcgcaggc
     2401 atgaccatcg ggatcgagtt acagaaaaaa ggtatcgagg tcaccattgc cgaaagacag
     2461 ccggatctga atgaaaaagg gcatgctttt ctcatgcatg acgatgccct gtcgatcctg
     2521 cgtgagctgg gcggcgatgg gaccgatctt cccggtcagt cggtcggcag gttttgcctc
     2581 cgaagaccca acggctatga agtaaagaac gtggagctcg actcctggcg ctgtatgaaa
     2641 agatgcgagc tgatcaattt tctccgcgga ttgctgaaac ccgataccat cgtgcgggga
     2701 agggcttttt cgcattttat ccggaaggac ggaaaggctg ttgccgccgt ttttgccaat
     2761 ggagacgtcg aatacggcga tatctttatc ggggccgacg gtggaaattc gaaggttcgg
     2821 gaggccttgt tcgggaaggt cgcgctgacc gctgtggccg taaaggagat cgtggggatc
     2881 tcgcacaatc gggagattgc cgcacggcat acgaatactt ttctgaagtt ccaggaaata
     2941 gaccgcggac tggctttcgg gatgataccc accagcgaga acgagttcgt ctggttcatg
     3001 cagtacgatc cgaccatcgg tgacgccccc gaggaccggc cggaaaagat acgggccttt
     3061 tgtaaccgta tgcttcgtgg attccccgag gtcaccgctg agatcctccg atcgaccgat
     3121 ttcagcaaaa catatatctg gagcaccagg gacttcgatc tgctccctgc cttccacagg
     3181 cagaatgtgg tcctgctggg agacgccgcg catctgacgc tgccctttac cagcgcaggc
     3241 acgaccaatg cgatcgttgg ctcgaaaata ctattgcaat gtctcgaaga caccgagaac
     3301 catgaagctg cattccggga ttattaccgt cagcggggtc cggtgatcga gaaacacatc
     3361 ctgttgggtc gtcagctcaa gaaaatattc cttttcccca gtggacgaga agagtctccg
     3421 ctgcccttcc tggcgtccag ccggaatgcc gaaggcgaaa aggtgacgat gatcaaacgg
     3481 gtgatccgat ccgggccacg cgaaaagttg ctccgggtga aatatttcac cgaccctatc
     3541 tgctccacct gctggatcat tcagcctatc tggaaaaagc tgagtctgga atacggccac
     3601 tatctcgaca tccagtacgt catgggcggc ttgctgcctt cctggaagga ttgcaagggc
     3661 aagatcatca gtccttcgga cgccgccata cattggaaag aggtgtccga ttcctataag
     3721 atgcccatcg acagcgatgt ctggatcgag gatccattgt cgagcagctg gcctccgtcg
     3781 attgcattca aggctgcgca gctgcaggat gagccgcggg cgctgctctt tttaagaagg
     3841 atcagggaga tgatcttcct cgaaaaaaag aacatcatga aatggagctt tttggaaatg
     3901 gcggctgtgg aagtgggtct cgatgtgcca cggttcaggt ccgactatgt caacgaagcc
     3961 agggccagct tcgacaagga ccttgaattg gcgagggtga tgaatgtgaa cagcttcccc
     4021 acgttgttct tcagcgacca ggcgggcaat gaagtccggt tgaaagggta ccagcactac
     4081 caggatttcg agcgggtgat cctgcaattg ctgccgtttg cgaaaagggc ggagatcgac
     4141 cggcagccgg aaagtctttt ccgcagtttt cccacgatgg tggacgcgga attcgcattg
     4201 ttgagcaatc tgatgagaaa ggacgcccga caggtattgt tgcagctgaa tcagcaggga
     4261 tttatcgaaa aggtggaatc cgggaatggg atcctctgga agatgaaata gaaggggcat
     4321 cggcgttccg ccgctggttg gcctggggcg cgggctcgcc gtcgccggtt cggcgccgtt
     4381 tgggggtggg cgcccgtcag aattctacat gccccctgac gccgaagaca tgcacgggcc
     4441 cgctcctgtc cttattatat cccggattgt ggacgaactc atagtccacg ctaagccaga
     4501 catgatcgaa cagtttggca ttgtaataag cctccagaat agcttcattt ccgtaattca
     4561 gcgcaccgtc gccgatgatg aacccatatc cgcctgcttt caggaaagcg cggtggtcgg
     4621 atgaaatgcc attgatcaca ccggcgatgc cggcgacatc ttcctttctt ttccactggg
     4681 tgcctttgaa attcagaccc gcatgcacgg tctggtcgat ctcggtaaaa gcccagctga
     4741 cgtatttccc gtcgttccat cccaggcgcg cgaagaggct gatatctcct gtaagctcct
     4801 gttccaggtt gagaccgata ccggtctttt ttccgccgta ggcggtcgct ttgccatccc
     4861 ccgagatcac ctgcagcaag ccggtgtcgc cgtcccgcaa ggcagcgagg ccttgctggt
     4921 aggaaggggc cctggaggcc gtatagctga agaggagccg cacgacgccg cttcgtttac
     4981 cgaaggtcag attatgttga aattcggcgg tctcggaatg cgccttaccg aatttatatt
     5041 ccatttcgga atgattggcg atcacgggga ccgcgacgct ggaaacgcgc agggcccagt
     5101 tcggttcgat ccattcgacc atggctccca tcgtgtatcc cttggtgttg gcaggataat
     5161 cccaggcgcc attgctcatc agcgaccagt tcaggaattg cgtacggggg tcatggctat
     5221 aggtgttgtt gtcataaaaa tcggacatcg agaactttcc agccgtgatg accagcctgt
     5281 ttgcaggaac cctgacggca acctggttct tgccgttgtc gaacgatacc gaatcggagc
     5341 ttccgagcgg gatcatctgc tgaagatagc cccttgctat ggatactacc ggggacgggt
     5401 ccccgatgcg gtacgtctcc ccgttgagcg ctccggcgat gccgagtgcg gaactgagcc
     5461 ccctgccgcc ggacagttcg ggattgaaat agaaggcagc gcccggccag agtttgcgtc
     5521 cgatgaataa cgtggaagta acgctgacgg cgccgggttc taccgtatcg gcgaagctgt
     5581 tgtcgccgct ataagccgta tggaaagatg tatggatctg atcgatgacc gtcatctgaa
     5641 aatgtacgct ccaatgggaa ttggagtcag ggatcacctg ctgtgagaat agttgttgtg
     5701 ataatgccaa agcgagcaga agtcccgtga gtcgtttcat gctgtcgatg ttgagtcagg
     5761 caacgcaaag gtatttattt ggctaatgcg tcctgttaaa tctgcgtcac caaattattg
     5821 caacgttgtt gccgttgggg ggtggcggca gggatgattg caacgttgcc gttgggggat
     5881 agcggcgcgg cttattgctg accgttgcgg ccgccgaccc ggctttgctc gtcgcctgcg
     5941 ccgcttcctc ttcgttgcac ccggggtgct atcgacgacc ccgcggtagc ggttattgta
     6001 tacattgacc gaggcgttga aggtccagga tctcatgagc tgtttgctgt agttgacgcc
     6061 cagaccgatg ttgcgctggg aagcgaggtt cccgctggtc tggatgatgg tgtaattaga
     6121 atcaccgtgc tctttgaatg tgagcagctc atcctcaaaa cgaaaagggt tcaggctttg
     6181 ataggagggg cgctcgatcc ttatcccgta cgatataccg aaggtattct tgtcgttggt
     6241 cttggcgaat ttgcccatct gctgatgcca atcgacatag gccgcattga tattttcctt
     6301 atagatgaaa tgattgctga cggaggtgtc cggctcccag gtcttcagtg tctcgtcgta
     6361 tagcgtgtag acggcattgt tgtccgtccg gacaaagctc gatttgatgc ccgcttgcac
     6421 cgcgaatatt gggatcaacc tgcggaaaat aatgttaaaa cgggatgaaa ggcgagaata
     6481 tcacaccgaa cgaacagggt agggtgctgc cgtacccgtg aacgacgcgg cttgagaggg
     6541 gccttcgaaa tacagcagag tcgcgaggaa ctttttcaca tgcccgcggc tttcggatgg
     6601 aaggtagttt tgaagcagcc agaaattgcg gcttcccgac ttgcggatgg catgattgac
     6661 cggtccttcg ccaccgttgt atgccgcgag caccagcaac cagtcaccat attcggcata
     6721 aagatctttc aggtacctgg ccgccgcgcg ggtgctttta tcgaattgcg tccgttcgtc
     6781 gcggtcgcct gtaactttca gaccgagcag acgcgccgtt tccggcatca gctgccaggg
     6841 tccaaccgca cccacacggg ataccgcgtc ggacttcaga tcggattcga tcaccgcgag
     6901 atatttcagc tggagcggca actcgaactg attgaaaacg gcatcggtaa tgatgaaacg
     6961 ggatccgtca tgtttcctga tggcggaaaa tatcattcga ttttcgagaa gatatgtttg
     7021 gacgaatttc cggtcgtgct tattaagatg caaagcatcg atggctgaaa aagagacatt
     7081 cttttccatc atgaacccac cgccgatgat agatggtgtc attaacagca ggaagaaggt
     7141 gatgactggg gctttgatca tgatgcccat tatccaatcc cggtgccagt ctgtaaataa
     7201 ctgatcgtta gtatgttacg tggctgtttt gaaccggttt tttcccaaaa agggacgact
     7261 ttttcattag ttgcggtcca gcagaccgac tgcggtgccg gcggcggatc gcaccgcatc
     7321 ggagggactg gtcagggctt ctttgaatac cggcatatat tcccggtccc ggagcttacc
     7381 cagcacggac agcgctgcgg cctttacgag atggttggag tcgctatgga tcaactgtac
     7441 caaaatcgga gatgcggcat tccggatcac cgggtttttc atatccagcg cagaaacagc
     7501 atttatccgg agcccataat aggggtcatt caacgccgcg atcaatattt cttgggacct
     7561 ggcatcggag gggtcttcga tggcggcatt tatcgcctgc gatcggtcga gatacagcgg
     7621 ggcatggaaa tactggaaga catattcgtc cagcgattgg tggtcggtga tcacagccac
     7681 taacactttg tcggcatcca cgttcactaa gtcgggtttc gacgacgcgt ggaaggagaa
     7741 gctgtccagt ttgttctcca tgcgaaccat atatcgttgt tttcgtccgc cggaatagat
     7801 gtcgatcgcc atcgggagca gatagggtcc accctcctgg gtttgtcgca gccagaccgt
     7861 ttccgttgcc gtagtatcgt tccaccggtg tcggatatcc agtataggat ggccggcgcg
     7921 ataataccac tgatcgaaga accagtgcag gtcctgaccg ctgacatcct cgagcgccag
     7981 gcgtagctgg tcgacttccg catttttgaa tgcattgaca tggaggtaat ggctcaaacc
     8041 gctgaaaaaa gccgaatcgc caaggtaatt gcgcagcata ttcaagaccc cgttgccttt
     8101 atagtaagtg aggacatcga acatatccag ctcgttacgg taatggtatc ggaccatggg
     8161 cgccgtatcg acggatttcg tcttcagata gatttgtacg tcatgtgccc ggagagcatc
     8221 gcccgcatcg cgcccgtatt ggtgttctgc ccacagtccg tcgctgaatt ccgcgaaggt
     8281 ttcgtcgaca gcctggttgc tccagctgcg gcaggtgacc agtgcaccga accattggtg
     8341 gaacaattca tgtacgatgg tgctttgccc tttgctgtaa taggcgtcaa gcagctgtcg
     8401 cggggtttgt tgcacgtatt cattgagcag agtggccgta gtgttttcca tgccgccgct
     8461 ggcatagtcc cggactacga tctgtgcata tttgttccag ggaaagtcgt aatgcaggat
     8521 acgggagaaa tagtcgatca tctccggcgt tgtcccga
//
LOCUS       DASYSV010000035         3738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1032188, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000035 DASYSV010000000
VERSION     DASYSV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032188"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VGQ51_01030"
     CDS             complement(<1..213)
                     /locus_tag="VGQ51_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEV9035163.1"
                     /translation="MDIHSATYVISSPDYTKGPAPDRPEYAFIGRSNVGKSSLINMLS
                     HNDRLAKTSGTPGKTQLINHFNIDNAW"
     gene            263..1084
                     /gene="ubiE"
                     /locus_tag="VGQ51_01035"
     CDS             263..1084
                     /gene="ubiE"
                     /locus_tag="VGQ51_01035"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEV9035164.1"
                     /translation="MTDFSHDKVVPFKDSGLGKKQQIAEMFDRIAFRYDFLNRFLSGG
                     TDLYWRRRAIRELMSGGGKQRPVRAGDGAHDAGVAGLRAAGASVLDVATGTGDMAILM
                     TRLLPGVQVTGIDISAGMLDIGREKVNRLRLGQKITLQMGDSEALEFPDEHFDAITVA
                     FGVRNFEHLEKGLSEMLRVLKPGGQLVVLECTQPKTPGVRQFYRWYMCLIAPRIGKMV
                     SSSREAYQYLNDSVKAFPEGAAFLQILDSCGYTDTRLRRLSLGVSTLYLGRKTGS"
     gene            1300..1980
                     /locus_tag="VGQ51_01040"
     CDS             1300..1980
                     /locus_tag="VGQ51_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV9035165.1"
                     /translation="MLANLSASAQLRDYLNLPDHDSKRYYLGIGFMYTNSHFQVTPHP
                     KFLQSDSVLSVTPGNSGGFGVSGMFTLRLAEHWELRLAFPEFIFANNTLAYHVTYPPS
                     GESSVATKQIQSLLLGFPMHVKFLSDRIDNFRVYMLGGFNYQYDLASNSSARKAQNLV
                     KLSPADLSVEAGIGFQFYFPVFILSPELKISEGIKNVHSRDPNLQYSNVIDKLKSRMI
                     VFSLIFEG"
     gene            2050..2832
                     /locus_tag="VGQ51_01045"
     CDS             2050..2832
                     /locus_tag="VGQ51_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV9035166.1"
                     /translation="METFTRTEELGDSLKPKASFIRTQEMVEDYAAKEEVVEILAPKP
                     KANGTYNRNDSYGRAQEVVNGLIHGGGILFGLAGLPVLTSLANVHHNTHAIIGGGIYG
                     FCFLLAFTVSTIYHFASEPKVKRIFKIFDHIGIYYFISGTYTPFLLVYMNNSFGMTLL
                     AVLWGLTALGTVFKVWYTGKFEILSTLIYLAMGWIMLAGGRQFFDVLPHNVLVFLFIG
                     AALYSIGVFFYLWDKYKYTHAVWHVFVLTAALCHYVAVLMAM"
     gene            complement(2875..>3738)
                     /locus_tag="VGQ51_01050"
     CDS             complement(2875..>3738)
                     /locus_tag="VGQ51_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035167.1"
                     /translation="VNQHLTGNDYEYPKKYGVIHHLSFNATPWLRLGVFGNVMFSRAD
                     HYEFSYLNPVIFLVSAQQQNGSPDKTTAGLDWTVNIGHATQFYGQLLIDEFIAHQIAH
                     YSNGYWANKQGLQLGIKYIDAFAVRNLDLQLETNVVRPYTYQHDDTVANYTHYNQPMA
                     HPLGANFFEFIGIVRYQPKYRWNVEAKAIYYKQGLDSAGVNFGGDIFENYLTRPRDYG
                     FKIGSGVPANCINLSGLVSWQCRENLFLDLSVQYRNYSVTDPGNVFHSSSSTLLTGGI
                     RINMFRRQYDY"
BASE COUNT          808 a          903 c         1066 g          961 t
ORIGIN      
        1 ccatgcgttg tcgatattga aatgattgat gagctgcgtc ttaccgggcg tgcctgaagt
       61 cttggccagc cgatcgttat ggctgagcat attgatcagc gaggatttgc cgacgttgct
      121 gcgaccgatg aaggcgtatt ccgggcggtc gggcgcgggg cctttcgtat agtcggggct
      181 gctgatcaca taggtcgccg agtggatgtc catgcctgca agatacatcc tcggcggagg
      241 aaatgattac ctttgcgatc ctatgaccga tttttcacat gacaaggttg tccccttcaa
      301 ggattccggg ctcggtaaga agcaacagat cgcggagatg ttcgatcgca tcgctttccg
      361 atatgatttc ctgaaccgtt ttctctcggg gggtaccgat ctgtactggc ggcggagggc
      421 gatcagggag ctgatgtcag ggggtgggaa gcagagacca gtgagggctg gcgacggggc
      481 gcatgatgca ggggtagcgg gccttcgggc tgcaggggcg tcggtgctcg atgtcgctac
      541 cgggacgggg gatatggcca tcctgatgac gcgtctgttg ccgggagtgc aggtgacggg
      601 tatcgatatc tccgcgggca tgctggacat cgggcgtgaa aaggtgaacc ggctgcggct
      661 gggacaaaag ataacccttc aaatgggtga cagtgaggcg cttgaattcc ccgacgaaca
      721 tttcgacgcc attacggtcg ccttcggggt tcgtaatttt gaacacctcg aaaagggatt
      781 gagcgagatg ttaagagttt tgaaaccggg cggtcaactg gtcgtattgg agtgtactca
      841 gccgaaaacc ccgggggtcc ggcaattcta tcgatggtat atgtgcctga tcgctccgcg
      901 catcggtaaa atggtatcca gcagccggga ggcctatcaa tacctgaacg attcggtaaa
      961 agccttcccg gaaggggcag cttttctcca aatcctggac tcctgcgggt atacggatac
     1021 ccggttgcgc cggctgagcc tgggagtttc cactttatat cttgggagga agacaggctc
     1081 ctgacctcgc cactggcaca attattggcc ggattctccg ttatttctaa tcaccccaaa
     1141 ggcaagccta tagatatttt ctactcttct atcgcatggc agaagcatcc ggacaaagcc
     1201 ggagtgtaaa ttattgattt taaattattt atgcatttac tggggttaca aaagcgaatc
     1261 aggtcctttt tggccgtact cttaataatg tccgcggggt tgctggcaaa tttgtccgct
     1321 tccgcccagc tgagggatta cctcaatctg cccgatcacg acagcaaacg atattacctg
     1381 ggcatcggtt tcatgtatac caattcgcat ttccaggtga ctccgcatcc caaattcctg
     1441 caaagcgaca gtgttctgtc ggtgaccccc ggcaactccg gagggttcgg cgtatcaggc
     1501 atgttcacct tgcggcttgc cgagcactgg gagctgcgcc tggccttccc ggaatttatt
     1561 ttcgcaaaca atacgctcgc ctatcatgta acctatccgc cgtcgggcga gagttcggta
     1621 gcgaccaaac agatccagtc attgctgctg ggattcccga tgcatgtgaa attcctttcc
     1681 gatcgaatcg ataatttccg cgtttatatg ctgggtgggt tcaattacca gtacgatctg
     1741 gcttcgaatt cgtccgcccg aaaggcgcaa aacctggtga agctgtcgcc ggccgatctg
     1801 agcgtcgaag cggggatcgg cttccagttc tatttcccgg tcttcatcct ttcccccgag
     1861 ctcaagatca gtgaggggat caaaaatgtt cattcgcgtg atcccaatct gcagtattca
     1921 aatgtgatcg ataagctgaa gtcgcggatg atcgtctttt ccttaatttt tgaaggttag
     1981 cgaaccgcag gacagtgtat gatgttttcc tagtgagggc tgtggcatta ttttttctaa
     2041 acagtgagta tggaaacctt tacaaggaca gaagagttgg gcgacagcct gaagccgaag
     2101 gccagtttta tccgtacaca ggaaatggtg gaagactatg cggcaaaaga ggaggttgtg
     2161 gaaatacttg ccccgaagcc gaaagccaat ggtacctata accgtaatga tagttatggc
     2221 cgtgcccagg aagtcgtcaa tggtcttatc cacggcggag gcatcctctt cggtctggcg
     2281 ggtctgccgg tgctgacgag cctcgcgaac gttcaccaca atacgcatgc gatcatcggc
     2341 gggggtatct atggtttttg ttttctgctg gcgtttaccg tcagtaccat ttaccatttc
     2401 gcttccgagc cgaaggtcaa acggatcttc aagatattcg atcatatcgg gatctattac
     2461 ttcatatcgg gcacgtatac accgttcctg ctcgtctaca tgaacaattc cttcggcatg
     2521 acgctgctgg ccgttttatg ggggctgacg gcgctgggta ccgtatttaa agtctggtac
     2581 acgggaaagt tcgagatcct ttctaccctc atctacctgg cgatgggctg gatcatgctc
     2641 gccggagggc ggcaattttt cgatgtattg ccccacaatg tactcgtctt tctctttatc
     2701 ggggcggcgc tgtactccat aggcgttttc ttttatctct gggataaata caagtatacc
     2761 catgcggtgt ggcatgtttt cgtcctcacg gctgcattgt gtcattatgt ggcagtgctg
     2821 atggcgatgt aagccacgat ccgcggctca cggcgcggtg gcaccgcgca gttcctaata
     2881 atcgtattgt ctcctgaaca tattgatccg gatgccgcct gtgagcagcg tggagctgga
     2941 gctatggaat acattgcccg ggtccgtaac cgaatagttg cggtactgga ccgacaggtc
     3001 gagaaagagg ttctccctgc attgccagga taccagaccc gagaggttga tacagttggc
     3061 gggtacgccg ctgccgatct tgaatccata atcgcgcgga cgcgtaagat agttctcgaa
     3121 gatatctcct ccgaaattga cgccggcaga gtccagaccc tgtttgtaat agatggcttt
     3181 cgcttccaca ttccacctgt attttggctg gtaacgtacg atgccgatga actcgaagaa
     3241 attggcgccc aacggatgtg ccatgggttg gttgtaatgg gtatagttgg cgacggtatc
     3301 gtcgtgctgg taggtgtagg gtcttacgac attggtctcg agctgaaggt cgagattcct
     3361 gaccgcgaag gcgtcgatgt atttgatccc cagttgaagt ccctgtttgt tggcccagta
     3421 gccattggaa taatgggcta tctggtgggc gatgaactcg tcgatcagca gctggccata
     3481 gaactgtgtc gcatggccga tattgacggt ccagtccaga ccggcggtgg tcttatcggg
     3541 gctgccattc tgttgttgcg ccgagacgag aaaaatgacg gggttgagat aggagaactc
     3601 gtaatggtcg gcacggctga acatcacgtt cccgaatacg cccagtctca accagggtgt
     3661 cgcgttgaag ctgagatgat ggatgacgcc atattttttg gggtattcgt aatcattacc
     3721 ggtaagatgc tggttgac
//
LOCUS       DASYSV010000036        24570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1417543, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000036 DASYSV010000000
VERSION     DASYSV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 24570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24570
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417543"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VGQ51_01055"
     CDS             complement(<1..744)
                     /locus_tag="VGQ51_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEV9035168.1"
                     /translation="MSIAGQNLKYLRKLRGWTQEEFAAKLGIKRSLLGAYEEERAEPR
                     LDVLETISDMFKLTLDELLRKELSDSKGGSYLSKRRTQKLLASSNEIQLVPVKAAAGY
                     LAGYADPEFLDELNTFTLPMMAPGTYRAFEIVGDSMLPTPSGSVIVGEKVDDLEELKS
                     NQTYIVVSRNEGIVYKRVMKNNKSRNKLTLISDNTAYQPYQVNSDDVIEVWKAQMIIS
                     KANTTHRWDVNQLANLVSNLQEQVSTLKKK"
     gene            1013..4354
                     /gene="secA"
                     /locus_tag="VGQ51_01060"
     CDS             1013..4354
                     /gene="secA"
                     /locus_tag="VGQ51_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV9035169.1"
                     /translation="MLGILSKIFGNKSVKDIKYIQPLVEEVNQHFASYQSISNDELRE
                     KTGEFKRRIREHLSETDTRIADLSKQAEELPFSDITGKDALYQEIDKLKKDRDDQIEE
                     ILETILPEAFAVVKETARRFTQNSEMVSTATDLDRALSVKKEHIRIDGQQSVYNNSWM
                     AAGSPITWNMVHYDVQIIGGTVLHQGKISEMATGEGKTLVSTLPAYLNALAGEGVHIV
                     TVNDYLARRDSEWNGTIFEWLGLTVDCIDKHEPNSEARRKAYLADITYGTNNEFGFDY
                     LRDNMVHSPDEMVQRKHHYAMVDEVDSVLIDDARTPLIISGPVPKGDDQQYHILKGRV
                     EKLFEAQRRVCNMFLVEAKKKLAEGNDDPKDGGLALMRAWRGLPKSGPLIKFLSEPGI
                     KVRLQKAENYYLSDQEKQMPTVDAELFFNIDEKNNSVNLTDKGIQMITREGEDPEFFV
                     LPDIGIKLAEIEKVESNNDEKLQRKETLLNDYAVKADRIHTVQQLLKAYTLFDKDVEY
                     VVMEGQVKIVDEQTGRILDGRRYSDGLHQAIEAKENVKIEAATQTYATVTLQNYFRMY
                     HKLAGMTGTAETEAAEFWDIYKLDVVTIPTNVPAIRKDHQDLVFKTKKEKYRAVIDEI
                     ERLRTAGRPTLVGTTNVEVSELLSRMLQVKKIPHNVLNAKQHAREAQVVAEAGLAGAV
                     TIATNMAGRGTDIKLGSGVKEAGGLAIIGTERHESRRVDRQLRGRSARQGDPGTSQFY
                     VSLEDDLMRMFGSDRIASLMDKMGYKEGEVIQHSMVTKSIERAQKKVEENNFGIRKRL
                     LEYDDVMNKQRNVVYKKRNHALFGDRLALDLDNAFYIVAEGLVNSFREGDDYEGFKLA
                     TIVNFSIDTKISQEEFLKGDERTISEKLYQEATQNYENKLSELRRQALPAFANIRATQ
                     GSHVENVVVPFTDGKKRINVLAGLQKTLTTEGRELSNAVERQITLGIIDEAWKEHLRD
                     MDDLKQSVQMAHLEQKDPLVIYKVEAFELFRRMDSDVNRDIISFLCHSGIQQSTQQQA
                     TIREGREQKTDMSKMRVRKDEMAAAGAGAPHSGAPTEGGPDYYEPNDPVKHEPVKVGP
                     KIGRNDPCPCGSGKKYKNCHGKDAA"
     gene            4385..4903
                     /locus_tag="VGQ51_01065"
     CDS             4385..4903
                     /locus_tag="VGQ51_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9035170.1"
                     /translation="MTFAKTTAPTSRILVASLVGTTIEFFDFYIYATAAVIVFPKLFF
                     PAADATSATLESLATFALAFFARPLGAALFGHFGDRKGRKATLVAALMTMGPSTVAIG
                     LLPTYHSIGILAPILLALFRLGQGLGLGGEWGGAVGVSPSSRDHADAQPNRCRTECRS
                     APVGLVAIEDAA"
     gene            complement(4808..6439)
                     /locus_tag="VGQ51_01070"
     CDS             complement(4808..6439)
                     /locus_tag="VGQ51_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HEV9035171.1"
                     /translation="MLKKIFCCLFINLTAMSALRAQTPGLNHHEELPMPVLIESNGRH
                     QLLVDGKPFFIFGGQAHNSSGWPALLPHVWSAVARLNANTLEVPIYWEQIEPEQGRFD
                     FSLIDTLLTQARDHDVRLVLLWFATWKNGSNHYMPSWMKLQPDRYPNCIGKDGKPIDS
                     PSPIADATLEADKKAFAAIMHHLRLADPRHTVILVQVENEPGSWGTVRDYSPAAQRLF
                     EKAVPGALLKADILRALGHPGAAKGTWQQVFGKDADEYFHAWHVASFIGAVAAAGKAE
                     YGLPLYANAALRDPLTNPPASYYESGGPTDNVIPIWKIAAPAIDILSPDIYLDGNDKC
                     LKVIDLYSRPDNALLVPECGLSPDKVKYIYPTLAHGGIGFSFFGIDGNGDHADRLDPA
                     PDLLSPIAKEYRLLAAPAALLSQWAFEDRIRAVVEPEDHSAQSVALPGWQATIKFGTG
                     RGDMRPNEHPTGKALIINLEKNVFLATGTLCRITFRPTGADSTKAWQYLQVEEGEYVD
                     GVFKSRRILNGDETDWGGPAFGSTPVWLRISVIAR"
     gene            6502..7569
                     /locus_tag="VGQ51_01075"
     CDS             6502..7569
                     /locus_tag="VGQ51_01075"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV9035172.1"
                     /translation="MGFSLLHASAIAQYVGLDQKAVRHLERLIKSDSSAKKQYSELAA
                     TADEALNEDPNPIDTIRSEGLLQGDPRKTATWAALADMRKMYDLALVYRVSGNKKYLK
                     AAARYIMAWAATTTTSRGDPIDDTNLDPAIGAFDMIKDDLPAEQTAAISDWLRRTADA
                     ELHSPYNRPERATSYNNWHSHRLKIVGEIAFTIGDTALQGYAIRGLKEQIARNLNPDG
                     PSADFITRDALHYQVYDLEPLLTLTIVLKKATGVDYYSWRSPTGSSIRRSVEWLLPYL
                     TGEKTHAEFVHSTVEFDRQRARNGEKTYQAGALFESRNGIPALILAAWFEAALMQPAR
                     QVLGTNVRYPTWQSVVNALKG"
     gene            7670..10240
                     /locus_tag="VGQ51_01080"
     CDS             7670..10240
                     /locus_tag="VGQ51_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEV9035173.1"
                     /translation="MMRKFLTALALVAGLSTAIAQQRPTNPNQPPAGGVPGGGRGGAA
                     AQRQEPKPYKEVITDKAVSHPGLFTVHRVEDKWYFEIPDSIFGRDILVSTRYSKMAAG
                     GNYGGEAVNLQTIQWQKGPSHTVFMKVVTIVNVAADSSQPIAQAVRNSNLNPIAAAFD
                     VKAYGKSADSTSGTVVIDVTDFFKGDNQPVSLTPAIKRRYSLAMLAADRSYIESIHTY
                     PINTEIKTVKTWTSSPAPAGPTMGGRNPMGELPAAVESGAVTIELNNSFLLLPKVPMR
                     KRPFDPRVGFFASRFTVYGDDEQRVEEDEFIHHWRLEPKDEDIEKWKRGELVEPKKQI
                     VYYIDPATPKKWRPFLIAGINDWNAAFEKAGFKNAIVGKEWPENDRTMSLEDARFSVI
                     RYFASDIENAYGPNVADPRSGEILESHIGWYHNVMKLVHDWYMIQTAAVDPRARRMKF
                     DDALMGDLIRFVSSHEVGHTLGLRHNMGSSSKTPVDKLRDKAWVEANGHTASIMDYAR
                     FNYVAQPEDNITEKGLYPRIGAYDKWAIQWGYGPIADTRSEEEDKKILNKLIIDSLGS
                     NPRLWFGGEGQNFDPRAQTEDLGDNAMKASDYGIKNLKRILPNLPEWCKEEADRYDNL
                     EEMYQQLVGQFSRYMGHVTKNVGGVQETFKSVEQSGDVYEATPKATQQEAVAFLNKQL
                     FATPTWLLNKDIVNKFSNPGTEDQLSTVQANTLKSLLSAARLYRLAVCTQRYGAGVYS
                     VDDLLTDAKKGVWGELATGAAIDPYRRNLQKQYVQALSVLINPEPPVQISLGNLPRGL
                     VFFTGDIRFTDVPSIARAQLVALRTEIGAAIAREGDKVSRYHLQDVSERISQALNPKK
                     "
     gene            complement(10251..12653)
                     /locus_tag="VGQ51_01085"
     CDS             complement(10251..12653)
                     /locus_tag="VGQ51_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HEV9035174.1"
                     /translation="MKLPILASAVLFCCQLNAQRTQLPNGWFLTPAGTGLPLSTDLPL
                     NIALSPDGVHAVVTNNGDGPQAIDLIDLRTRRVTASVPMNRAWLGLAFARRHPWLYAS
                     GGNDNTIIRFTLSGDTLVRPDTLVLGKPWPAANISPAGLCLDETRDRLYVVTKENDAL
                     YVFDTRTKRLLMRTPLGAEAYTCVLSPTGKELYIAAWGARRIWIYDTKQDRLRDSVAT
                     EDHPTDMTLDRHGRMLYVANANSNSVSVIDVAHRRVLETLHTALSPEAPIGSTTNSVA
                     LDAQDHTLYIANADNNYLAVFDVSEPGHSRSLGFIPVGWYPTCVRMSAGTLLVANGKG
                     FTSMPDPYGPSPLEHGDAFKKSHGHDEQYIGNMFKGTLSAIPTPSAAELSAYTQQVYA
                     NSLYTRTREQTAPGQEHNPIPRSAGEPSPIRYVFYVLKENRTYDQVLGDDPRGNGDTS
                     LVLFGKTITPNAHAIVEDFVLLDNFYVDAEVSADGHNWSMAGYATDFTEKTWPTNYSG
                     RGGDYDFDGHRAVANPTNGFIWDYCARAGVSFRNYGEFMDDGHPTLPVLKIPANYCFK
                     YPGWNLDIQDIYRESVFEHDFDSLVAIGAVPHFSTVYLPNDHTSGLNKGSYTPVAQVA
                     DNDLALGRLVDHISHSPIWGESAIFVLEDDAQDGPDHVDAHRSPAYVISPWVRRHSVD
                     HTLYSTTSILRTMELILGLPPMSQFDAASTPLYSSFTSTRDTTPFHALPAGVDIKLRN
                     AGEGPSAQISRHFNLSDPDRIPDRLMNAVIWHALKGENSPVPQPRRGAFVQEKAPAKR
                     GP"
     gene            complement(12710..14035)
                     /locus_tag="VGQ51_01090"
     CDS             complement(12710..14035)
                     /locus_tag="VGQ51_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9035175.1"
                     /translation="MKLSYKLLFCTLLSAATAMAQDDVYPAKPYAGKLIITGGTIHVG
                     NGQVIEDGTIVVEKGKIVSVGAQAPPNTEGAKVVDAKGKQIYPGLILPVTDLGLKEIG
                     NGVRGSNDYLELGDLNPSVRSIVAYNTDSKIIGTLRANGILLAGVTPEGGTISGSSTI
                     VQLDAWNWEDAAYKMENAIHVNLPSFMNRPRRFGGGGGGRFAFGQQAGDPTKEALAKI
                     DELKAFFSEAKAYAAEPGHKETNLKFEAVKGLFNHTQKLFVHGDQVRQMLVAIDFAKE
                     FGMDVTIVGGSESFLIADLLKKNNISVILQQLHALPTTEDDDVDQPFKTPAVLQKAGV
                     LFSLNDNHEESRYRNLAFNAGTAAAYGLTKEQALQAITLNTAKICGIDDRTGSLEPGK
                     DANIVISGGDILDMRTSLIEHCFIQGREVSLENKQTQLYHRYMTKYGLE"
     gene            complement(14047..17193)
                     /locus_tag="VGQ51_01095"
     CDS             complement(14047..17193)
                     /locus_tag="VGQ51_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9035176.1"
                     /translation="MRNARHRPRGTILYLSTALLFLTLFTSAQSTFPVNGVADPRVGC
                     YAFTHATLVKDEGNTISDGTLIIRDGTIVGAGTGLTPPKDAVVVDCSGKYIYPSFIDL
                     YSDYGIAEPEVPGRGGRGAAFDPAAFFRQPAQIGSATKGAYNWNQAVRPETDASHIFV
                     VNETAAKPLRDIGFGTVLTQKKDGIVRGTGVVVTLGNQNENLVIVKQRASANYSLNKG
                     TSTQSYPSSLMGSIALLRQTYLDASWYKNQNYHEGTNLSLQAFNDEQTLPQIFDGDDK
                     WGDLHGDRIGDEFGVQYIIKAGENEYQRMHEIAATKAAYIVPVNFPQAMDVEDPDAAR
                     FVSLADMKHWELAPTNPGAFEKAGITFCLTASDLRDPRQFFTNLRKAFDNGLSEARAL
                     EALTKTPATLIGIYDKVGSLDAGKLANFLITNGPIFSEKTSIVENWVQGRKYGVKEEA
                     WKDVRGQYNLVITSPSGASSTYVLDVKSPTSASVIGKDTLSGKFSFDGKLVALTFGGA
                     TGATPAPRTPGEAAPRGRSFGGGRPGAATHLTGTLNGDIFNGNGVDSAGGLVVWTASF
                     TRALAPDSGSTPRRRQQRLGKVLYPFDGYGWDSLPTAHDLLIRNATVWTNEKEGRLAN
                     TDVLVKNGKIAKIGKDLSAGDAQVIDGSGKWLAPGIIDEHSHIAAFSINEGAQSVTSE
                     VRIADNLNPEDISIYRCLAGGVTSMHILHGSANTIGGQTQLIKLRWGANDTALKFRNW
                     DPFIKFALGENVKRTTSTNNNRFPDTRMGVEEVLMDAFTRARDYEKARKAAPGATRRD
                     LELDALVEILNHKRFITCHSYVQSEITALMRVADKFGFTVNTFTHILEGYKVADKMKA
                     HGSNASTFSDWWAYKMEVQDAIAYNAAIMTKEGLNVCINSDDAEQARRLNQEAAKSVK
                     YGGLSEEEAFKMVTLNPAKALHVADRVGSIREGKDADLVLWSDNPLSIYARAEKTIVD
                     GIVYYDVDHDARMRKYIATERNRLVQKLVAEKRTGTPMAQAMPSYRVELSCGDHDHHD
                     GLLTIDVSDGDDDTNGTN"
     gene            17299..17943
                     /gene="deoC"
                     /locus_tag="VGQ51_01100"
     CDS             17299..17943
                     /gene="deoC"
                     /locus_tag="VGQ51_01100"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV9035177.1"
                     /translation="MSELAHYIDHTILKPTTTHIDINKICQETREYGFAAACVPPSFV
                     RLARQAGVRVATVIGFPFGYSFTRAKLAEIEQALSDGADELDVVINLTALKDGDWGYL
                     ETEMRLLMEPIHTEGRLLKVIIESGVLTEAEIVRCCEIYGGMGVDFVKTSTGYAEQGA
                     TLGAVRLMRAQLPASVQIKASGGIRTAALAQQFIEAGASRLGTSSGVTLIKSYV"
     gene            17936..18376
                     /locus_tag="VGQ51_01105"
     CDS             17936..18376
                     /locus_tag="VGQ51_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEV9035178.1"
                     /translation="MYKSLIVLLLVAGRAAAQTDSGGVNVTVDPRVDQLIKKQISINE
                     ATTLEARRHVPGFRIQVINTPDRNKVNAAKIKVYEQFPDWRPYLIYQAPNYKLRVGDF
                     KTEDEANAAMQQLSRLFPSGLYVIRDIIEVKLSDVQKMDSTNNR"
     gene            18392..19579
                     /locus_tag="VGQ51_01110"
     CDS             18392..19579
                     /locus_tag="VGQ51_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEV9035179.1"
                     /translation="MLKDTIRQLAKIYSPDLIAIRHHLHAHPELSYKEYETSAFVQRQ
                     LEVLGIPFRIMAGTGVVGLLEGRNPKSRVIALRADMDALPIREENKVEYRSKNEGVMH
                     ACGHDVHTTVLLGAARILNDLKGDWEGTIKLIFQPGEEKNPGGASLLIKEGVLKDPEP
                     QAIFGLHVHPGLETGKLSFRGGMVMASADELYITIKGKGGHAAAPHQTVDTILVASQL
                     VVALQQIISRNRNPLSPSVLSITSFQGGHTTNVIPSEVKLMGTFRAMDEEWRFRAHEL
                     IRQLATQLVHSMGAEIDLHIDVGYPMVYNNEALDRVARTAAREYMGADHVLETEVRMG
                     AEDFGYYTRLVPGCFYRLGVMNVAKGITSGVHTPTFDIDESAIEIGAGMMAWLGANIR
                     FEQ"
     gene            19611..21947
                     /locus_tag="VGQ51_01115"
     CDS             19611..21947
                     /locus_tag="VGQ51_01115"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEV9035180.1"
                     /translation="MTQKKQLRKEQALEYHSKGRPGKIEVIPTKEAKTQRDLSLAYSP
                     GVAEPCKEIHKNVEEVYKYTAKGNLVAVISNGTAVLGLGDIGPEASKPVMEGKGVLFK
                     IFADIDVFDIEINEKDPERFVQIVKALEPTFGGINLEDIKAPECFYIERSLREQLKIP
                     VMHDDQHGTAIISSAALLNALELQKKKIDKVRFVINGAGAAAMACVKLYVALGAKPEN
                     FTLFDKDGVLHEDRPDLDEDKKVFAVKKADLTLDKALKDADVFVGLSVGNVVTAEMVK
                     SMARKPIVFAMANPDPEIAYDIATASREDIIMATGRSDYPNQVNNVLGFPFIFRGALD
                     VRATQINEAMKLAAVKALADLAKTPVPDIVNMAYNEKNLSFSATYIIPKPLDPRLLST
                     VAPAVARAAIESGVAQHQIDNWEAYTIELNRRLGLDNQLMRVMSSKARLNPKRVVFAE
                     ADNIKILKVAQLLYDEGVAFPMLLGDERKIRHIASMNQIDLEGMPVINPYNDEYEEQR
                     KKFGELFFQKRQRRGFNKYEAYKTMRNRNYFGCMMVETGEADAMISGLTKNYPDTIKP
                     ALQAIGTEEGVNKIAGMYLLLTKRGPLFLADTTVNFNPTTEELADITLLVAREVQNFN
                     LTPRVAMLSYSNFGTSTSPEAKLVADAREIVKRKNPQLIVDGEMQASVALNNDILREN
                     YPFSELVDQEVNTLVFPNLAAGNIAYNLLKEVGGADAIGPILLGLKKPVHVLQLGSTV
                     RQIYNMALVAIVDAQIKCKNNATDEEAKKAPWWKRTRN"
     gene            21958..22695
                     /locus_tag="VGQ51_01120"
     CDS             21958..22695
                     /locus_tag="VGQ51_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035181.1"
                     /translation="MNFFTEFGSYLLTLKGMFTKPENGKMYWKEFMHQCSEIGIGSLG
                     IVAIISMFMGAVSTLQTAYQLVSPLIPLSTIAQIVRDTVILEFSPTLVCIVLAGVVGS
                     KIASELGNMRVSEQIDALEIMGINTKTYLILPKLLAAIMVIPMLVVLSAVLGIWGGRL
                     AGTLTGILSKEMFDKGLHQYFKPYNVFFAMAKAYTFAFIISSIPAYYGYNVSGGALEI
                     GRASTRSVVVSCILVLFADYLLSALLL"
     gene            22883..23692
                     /locus_tag="VGQ51_01125"
     CDS             22883..23692
                     /locus_tag="VGQ51_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9035182.1"
                     /translation="MIEIKNIKKGFGEKSVINDVSTVFKTGKCNLIIGTSGSGKTVMM
                     KCLVGLMEPDSGEIIYDGQNFSEMNEEGRKELRQSIGMLFQGSALFDSMTVEQNIMFP
                     LDMFTKNTFAEKLRRVNEVLDRVNLQGANKKFPAEISGGMKKRVGIARAIVLNPKYLF
                     CDEPNSGLDPQTSLLIDKLIKEITLEYNITTVVNTHDMNSVMEVGDHIMYMHQGVKEW
                     EGTNKEIIFSKNKLLNDFIFASEFLRDAKDMRMLEVRGKIDNDRNMDELLK"
     gene            23706..>24570
                     /gene="sucD"
                     /locus_tag="VGQ51_01130"
     CDS             23706..>24570
                     /gene="sucD"
                     /locus_tag="VGQ51_01130"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEV9035183.1"
                     /translation="MSILVNKHSKIIVQGFTGTEGTFHATQMIDYGTQVVGGVTPGKG
                     GSTHIERPVFNTVEAAVKATGADVSIIFVPPAFAADAIMEAAEAGIGLVICITEGIPV
                     QDMVKAKNYLLGKNTRLIGPNCPGVITAGECKVGIMPGFVFKTGKIGIVSKSGTLTYE
                     AADQVAKAGLGISTAVGIGGDPIIGTPTKEAVELLMNDPGTEGIVMIGEIGGSMEAEA
                     ARWISQNNRKPVVGFIAGQTAPPGRRMGHAGAIIGGADDTAAAKMKIMAECGITVVQT
                     PAEIGSTMAKVL"
BASE COUNT         5677 a         6618 c         7083 g         5192 t
ORIGIN      
        1 cttctttttt aacgtgctga cctgttcctg aagattgctc acgagattgg ccagctggtt
       61 gacgtcccag cggtgagtcg tattggcctt actgatgatc atctgggcct tccacacttc
      121 gatgacgtcg tccgagttga cctggtaggg ctgatatgcc gtattgtcgc ttatcagggt
      181 cagcttattg cgggatttgt tatttttcat gaccctttta tagacgatcc cctcattgcg
      241 tgagacgacg atataggtct gattgctctt gagctcctcg agatcgtcca ccttttctcc
      301 cacgatgacc gaaccgctgg gggtcggcag catcgaatcc ccgacgattt cgaaggcccg
      361 ataagtgccc ggcgccatca tcggcagggt gaaagtattc agctcatcca aaaattcagg
      421 atcggcataa cctgccagat agccggccgc ggccttcacc ggcaccaact ggatctcgtt
      481 ggaggaagcc agcagcttct gggtcctccg tttggaaaga taactgccgc ctttggaatc
      541 actgagctct ttgcgtagca attcgtcgag cgtcaatttg aacatgtccg atatggtctc
      601 cagcacgtcg agacggggtt ccgccctctc ttcttcataa gccccgagca gcgagcgctt
      661 aattcctaat ttggcggcaa attcttcctg tgtccagccc cgcagcttgc ggaggtattt
      721 gaggttttgg cctgcaatag acataaacta acatatttag cactaagata ctaacatatt
      781 taacagcgca aaaaaatatt tttccgcagt ggcaacatct tatttttcca ccccggcgca
      841 gcacggcgac agtcaatgaa aaggccgatc caccggcggg caggaggcca gttttttcgt
      901 acatttgctt ccctgccaat ttggcctttt gaggaagata gcaccaaatt tgaaggacta
      961 tttcgcggcc tgcctcggac gcgaaacaca aactattaat aattaaggaa ttatgttagg
     1021 tatactctcc aaaatatttg ggaacaagtc tgtaaaggac atcaaatata tccagcctct
     1081 tgtcgaggag gtcaaccagc attttgcctc ttaccaatcc atcagcaatg acgaattgcg
     1141 ggaaaagacc ggtgagttca aacgccgtat ccgcgaacat ctcagcgaga ccgataccag
     1201 gatcgccgat ctgagcaaac aggcggaaga gctccccttc agcgatatca ccggcaagga
     1261 cgccctttac caggagatag acaagctgaa aaaggaccgt gacgaccaga tcgaagagat
     1321 cctcgaaacc atacttcccg aagcgttcgc cgtcgtcaaa gagacggccc ggcgctttac
     1381 gcagaattcc gagatggtct ccacggccac cgatctggat cgtgcgctca gcgtaaaaaa
     1441 ggaacatatc cggatcgacg gtcagcagtc cgtatacaac aactcctgga tggccgccgg
     1501 cagccccatc acctggaaca tggtgcatta cgatgtacag atcatcggcg gtacggtcct
     1561 ccaccagggg aagatctccg aaatggccac gggggaaggt aagacgctgg tgtcgaccct
     1621 tcccgcctat ctgaacgcgc tggcgggaga aggggtccat atcgtgacgg tgaacgatta
     1681 cctggcccgg cgcgactccg aatggaacgg caccatcttc gagtggctgg gtctgaccgt
     1741 cgattgtatc gataaacacg aacccaacag cgaagcccgc cgcaaggcct atcttgccga
     1801 tatcacctat ggcactaaca acgaattcgg cttcgactat cttcgcgata acatggtcca
     1861 ttcccccgat gagatggtcc agcgtaaaca ccactatgcg atggtggatg aggtggacag
     1921 cgtactgatc gacgacgccc ggacaccgct gatcatatcg ggtcccgtcc ccaagggcga
     1981 tgaccagcaa tatcatattc tgaaaggccg cgtggaaaag ttgttcgaag cccagcgccg
     2041 cgtgtgcaac atgttcctcg tcgaggccaa gaagaaacta gccgaaggga acgacgatcc
     2101 caaagacgga ggcctcgcgc tcatgcgcgc ctggagaggt ctgccgaaat ccggcccgct
     2161 gatcaaattc ctcagtgagc ccggcatcaa ggtccggttg cagaaagcgg agaactacta
     2221 cctgtccgac caggaaaagc aaatgcccac agtcgacgct gaattatttt tcaacatcga
     2281 cgaaaagaac aattcggtga acctgacgga caagggcatt caaatgatca cgcgcgaagg
     2341 cgaggacccg gagttctttg tcctgcccga tatcggcatc aaactggccg agatcgaaaa
     2401 ggtcgagagc aacaatgatg aaaagctaca acgcaaggaa accctcctta acgattatgc
     2461 cgtaaaggcg gaccgcattc ataccgttca gcagttgctg aaagcctata ccctgttcga
     2521 caaggacgta gaatatgtcg tgatggaagg gcaggtgaag atcgtcgacg agcagacggg
     2581 tcgtatactg gatggaaggc gttatagcga tggtctgcac caggccatcg aggcgaagga
     2641 gaacgtgaag atcgaagccg ccacgcagac ctatgctacg gtgacacttc agaactattt
     2701 ccggatgtat cacaagctgg ccggtatgac cggtacggcg gaaacggaag ccgcggagtt
     2761 ctgggacatc tacaaactgg acgtggtgac gatccctacc aacgttcccg ccatccgtaa
     2821 ggaccaccag gacctcgtgt tcaagaccaa aaaggaaaaa tacagggccg tgatcgacga
     2881 gatcgagcgg ctgcggaccg cggggcggcc caccctggtg ggtacgacca atgtagaagt
     2941 gagcgagctg ctgagcagga tgctgcaggt aaaaaagatc ccgcataatg tactaaacgc
     3001 caaacagcac gcgcgggagg cacaggtcgt cgcggaagcc ggtctggcgg gcgcagtgac
     3061 catcgccacc aatatggcgg gtcgtggtac ggatatcaaa ttagggtccg gtgtaaagga
     3121 agcgggaggt ctggcgatca tcggtaccga gcggcatgaa agccgccgtg tcgacagaca
     3181 gctgagaggt cgttcggccc gtcagggtga ccccggcaca tcgcagttct atgtttcgct
     3241 ggaagacgat ctgatgcgta tgttcggcag cgacaggatc gcttcgctga tggataagat
     3301 ggggtataag gaaggagagg tcattcagca cagcatggtg accaagagca tcgagcgggc
     3361 gcagaaaaag gtcgaagaga acaacttcgg catccgcaaa cgtctgctcg aatatgacga
     3421 cgtcatgaac aaacagcgga acgtcgttta taaaaagcgt aatcacgcgt tgttcggaga
     3481 caggctggcg ctggacctgg acaatgcctt ctatatcgtg gccgaagggt tggtcaactc
     3541 cttccgcgaa ggggatgact atgaagggtt caagctggcg acgatcgtca atttcagtat
     3601 cgatacgaag atcagccagg aagaattcct gaaaggcgat gagcgcacga tctcggagaa
     3661 attgtaccag gaggccacgc agaattacga gaacaaattg tccgagctgc gcagacaggc
     3721 gttgccggca ttcgcaaata tccgcgccac ccagggcagc catgtggaga atgtggtcgt
     3781 gccgttcacg gacgggaaaa aacgaatcaa cgtgctggcc ggtcttcaga agaccctgac
     3841 gaccgaagga cgcgagttgt ccaatgcggt agaacgtcag atcacgctgg gtatcatcga
     3901 cgaagcgtgg aaagagcatc tgcgtgatat ggacgatctg aaacagagcg ttcagatggc
     3961 gcacctcgag caaaaggacc cattggtgat ctataaggtg gaggccttcg agcttttccg
     4021 ccggatggac agcgacgtca acagggatat catcagcttc ctctgtcaca gcggtatcca
     4081 gcaatccacc caacagcagg ccaccatccg cgaggggcgc gagcagaaga cggatatgag
     4141 caaaatgcgc gtgcgcaagg acgagatggc cgcggcgggc gcaggtgcgc cgcatagtgg
     4201 tgcgcccacc gaaggaggtc cggattatta cgagccgaat gaccccgtca aacacgagcc
     4261 cgtgaaagtg gggccgaaga tcgggcgtaa tgatccctgt ccgtgcggta gcggaaagaa
     4321 atacaagaat tgccatggga aggatgcggc atagccgatc cgcggtaata ttgtaagttt
     4381 gggaatgact ttcgcaaaaa cgacagcgcc aacgagccgt atcctcgtag ccagcctcgt
     4441 cgggaccacc atcgagtttt ttgacttcta tatctatgcg accgcggcgg tgatcgtctt
     4501 ccctaaatta ttttttcccg cggccgatgc cacgtcggca accttggaat ccctggccac
     4561 ctttgcgctg gcttttttcg ccagaccgct gggcgccgcg ctgttcgggc atttcggcga
     4621 ccggaagggc cggaaagcca cgctggttgc agctttgatg accatggggc cttctaccgt
     4681 cgcgatcggt cttttaccta cgtatcattc gatcggcatt ttggctccga tcctgctggc
     4741 cctttttcga ctgggccagg gtctgggtct tggcggagaa tggggcggcg cggtgggagt
     4801 aagcccatca tcgcgcgatc acgctgatgc gcagccaaac cggtgtcgaa ccgaatgcag
     4861 gtccgcccca gtcggtctcg tcgccattga ggatgcggcg tgatttgaag acgccgtcca
     4921 cgtactcgcc ttcttctacc tgcaaatatt gccaggcttt ggtgctgtct gcgccggtgg
     4981 ggcgaaacgt gatgcggcat agcgtgccgg tcgcgaggaa gacatttttt tccagattaa
     5041 tgatcagcgc ctttccggta gggtgctcat tgggacgcat gtctcccctg cccgttccga
     5101 atttgatggt ggcctgccag ccgggcaagg ccacggattg cgcggaatgg tcttcgggtt
     5161 cgacgaccgc gcggatcctg tcttcgaatg cccattggga tagcagtgcg gcgggggcgg
     5221 cgagaagccg atactctttg gcgatcggcg agaggaggtc gggcgccggg tcgaggcggt
     5281 ccgcatgatc gccattgccg tcgatgccga agaatgaaaa tccgataccg ccatgtgcga
     5341 gcgtgggata gatgtatttg accttgtccg gcgacagacc gcattcaggc acgagcagtg
     5401 cattgtcggg acgcgagtag agatcgatca ctttcagaca tttgtcattg ccgtcgagat
     5461 aaatgtcggg agacaggata tctatcgctg gtgccgcaat cttccagatg ggtatgacat
     5521 tatcggtggg accgccactt tcataatagg aagcgggggg attcgtcaac ggatcgcgaa
     5581 gtgcggcatt ggcatacagg ggcaaaccat attccgcctt ccctgcggcc gcgacggccc
     5641 cgataaaaga ggcgacatgc caggcatgga aatattcatc cgcgtccttg ccgaacacct
     5701 gttgccaggt gcccttcgcg gcaccggggt ggccgagcgc tctcagaata tcggctttca
     5761 ataacgcccc gggtacggct ttttcgaata atcgttgcgc ggcgggcgaa tagtctcgaa
     5821 ccgtccccca tgagcctggc tcgttctcca cctgcacaag gatcaccgta tgccgcgggt
     5881 ccgcgagccg gagatggtgc atgatggcgg caaaggcttt tttgtccgcc tccaatgtcg
     5941 catcggcaat gggtgacggg ctgtcgatgg gtttcccgtc tttgccgatg caattcggat
     6001 agcggtctgg ctggagcttc atccaggagg gcatgtagtg gttggaaccg tttttccagg
     6061 tggcgaacca gaggagcacg aggcggacgt cgtgatcgcg ggcctgcgtc aacagcgtat
     6121 cgatcagtga gaaatcgaat cgcccctgtt ccggctcgat ctgttcccaa taaatcggga
     6181 cttcaagggt gttggcgttc agtcttgcca ccgccgacca gacatgcggg agaagtgcag
     6241 gccatccgct cgaattatgc gcctggccgc cgaagatgaa gaagggtttg ccgtcgacca
     6301 gcagctgatg gcggccgttg gattcgatca acacgggcat ggggagctcc tcatgatgat
     6361 ttagcccggg cgtctgcgcc cgaagggcag acatcgccgt gagatttatg aacaggcagc
     6421 agaatatctt ttttagcatg aaagcaagtt ccttgaattt ttgtacattc cgacatgatc
     6481 aggcctgcta taatcttgct tttgggtttt agccttctgc acgcaagtgc cattgctcag
     6541 tacgtcggcc tggatcagaa agcggtccgg cacctggagc gtttgatcaa gtccgacagc
     6601 agcgcaaaga aacaatatag cgaactggcg gctacggccg atgaagcatt gaacgaagac
     6661 cctaacccca tcgacaccat ccggagcgaa ggactgttac agggtgatcc gcgtaagacc
     6721 gccacctggg ccgcattggc ggatatgcgt aaaatgtatg atctggcgtt ggtctatcgg
     6781 gtttctggaa acaagaaata cctcaaagcc gccgcccgat atatcatggc ctgggcggca
     6841 accaccacca cgtcccgcgg cgatccgatc gacgacacca acctcgaccc cgccatcggc
     6901 gctttcgata tgatcaaaga tgatctgccc gcagaacaga cggctgccat ttccgactgg
     6961 ctccgtcgca cggcagatgc cgagctccat tctccttaca acaggcccga acgcgcgacc
     7021 tcctataaca actggcattc ccaccggctg aagatcgtgg gtgagatcgc ctttaccatc
     7081 ggggacaccg cattgcaggg ttatgcgatc cgaggcctga aagaacagat cgcgcgcaat
     7141 ctcaatcccg acggccccag cgccgacttt attacgcgcg atgccctgca ttaccaagtc
     7201 tatgatctcg aaccgttgct gacgctgacg atagtcctga aaaaagccac cggcgtcgat
     7261 tattattcct ggagatcgcc cacgggatcc tctatacgcc gatcggtcga atggctgctg
     7321 ccgtacctca ccggcgaaaa gacgcatgcc gaattcgtac atagtaccgt cgagttcgat
     7381 cggcagcgcg ccaggaatgg cgagaagacc taccaggccg gcgccctgtt cgagtccagg
     7441 aacggtatac cggccctgat cctggcggcg tggttcgagg ccgcgttgat gcagcctgcc
     7501 cggcaggtat tggggacaaa tgtccgttat ccgacatggc agagcgtggt aaatgccttg
     7561 aaggggtgaa gggcggcggc cgcatgcggt gctgaacgca ccggggggct gatgaacggg
     7621 tcggtggggc caattgtttt ccctaatttg ggcgcttaaa ctcccttaca tgatgagaaa
     7681 atttctgact gcgctggcgc ttgtcgccgg tctttcgacg gccattgcgc agcaacgacc
     7741 aactaacccg aaccaacccc ctgcaggcgg agtcccgggc ggcggacgtg gtggcgcagc
     7801 cgcacaacgg caggaaccca agccttataa agaagtgatc accgacaagg cggtgagcca
     7861 tcccggtctt tttactgttc atcgcgtaga agacaaatgg tatttcgaga tacccgactc
     7921 tatcttcggc cgggacatcc ttgtgagcac ccggtatagc aagatggccg cagggggcaa
     7981 ttatggcggc gaggccgtga atttgcagac gatccagtgg cagaagggtc cttcacacac
     8041 cgtgtttatg aaggtggtga cgatcgtgaa tgtcgccgcc gatagcagcc agcccatcgc
     8101 ccaggcggtg cgcaactcca acctgaaccc gatagccgcc gccttcgatg tgaaggcata
     8161 tggcaaaagc gccgattcca cttcgggtac cgtggtcatc gacgtgaccg atttcttcaa
     8221 gggtgacaat caacccgtca gcctgacgcc cgccatcaaa agaagatata gcctggcgat
     8281 gcttgctgct gacaggtctt atatcgagtc catacatacc tacccgatca ataccgagat
     8341 caagacggta aagacctgga cttccagccc agctcccgcg ggacccacga tgggcggacg
     8401 caatcccatg ggtgagctgc cggcggccgt ggagagcggt gccgtgacca tcgagctcaa
     8461 caattccttc ctgctccttc ccaaagtacc gatgcgcaaa cggccgttcg atccgcgggt
     8521 cggctttttt gccagccgtt ttaccgtata cggtgacgac gaacagcggg tcgaggagga
     8581 cgagttcatt catcactggc gtctggagcc gaaagatgag gacatcgaga aatggaaacg
     8641 cggcgagttg gtcgagccta aaaaacagat cgtgtattat atcgatcccg ccacgcccaa
     8701 aaaatggcgt cccttcctga tcgcgggcat caacgactgg aatgccgcct tcgagaaggc
     8761 cggtttcaag aatgcgatcg tcggcaagga atggccggaa aacgacagga ccatgtcgct
     8821 ggaagacgcg cggttctccg ttatccgtta tttcgccagc gatatcgaaa atgcctatgg
     8881 tcccaacgtc gccgatccgc gcagcgggga gatcctcgaa tcgcatatcg ggtggtatca
     8941 taatgtgatg aagctggtgc atgactggta tatgatccag acagcagccg tcgatccgcg
     9001 agcgcggaga atgaagttcg acgatgccct gatgggcgac ctgatccgtt tcgtctcttc
     9061 gcatgaagta gggcatacgc ttggtctgcg ccacaacatg ggcagtagca gcaagacccc
     9121 cgtcgacaag ctccgggaca aagcctgggt ggaagccaac ggacatacgg cctcgatcat
     9181 ggactatgcg cgtttcaact atgtcgcaca gccggaagac aatatcaccg aaaaaggttt
     9241 gtatccccgc ataggcgcct atgacaaatg ggccatccaa tggggttatg gtccgatcgc
     9301 ggacacccgc agcgaggaag aggacaaaaa gatcctcaac aagctgatca tcgacagtct
     9361 cggctccaat ccgcggctat ggttcggggg agagggccag aatttcgatc cccgcgccca
     9421 gaccgaagac ctcggcgaca atgcgatgaa ggcaagcgat tacgggatca aaaatctgaa
     9481 acgcattctt cccaatctgc ctgaatggtg caaggaggaa gctgaccgct atgacaatct
     9541 cgaagaaatg tatcagcagc tggtggggca gttctcccgg tacatgggac acgtgacgaa
     9601 gaacgtcggc ggcgtacagg agaccttcaa gagtgtagag cagtcaggag atgtttatga
     9661 ggccactccg aaggccacgc agcaggaagc cgtcgccttt ttgaacaaac agctgttcgc
     9721 gacgccgacg tggttgttga ataaagatat tgtgaataag ttcagcaatc cgggtacgga
     9781 agatcagctg agcacggtgc aggccaatac gctgaagagc ctgttgtcgg ccgcgcggct
     9841 ttatcgtctg gcggtgtgta cgcagcgtta tggcgccggc gtatatagcg tggacgatct
     9901 gctgacggat gcgaaaaaag gtgtttgggg cgagctggca acaggcgccg ccatcgatcc
     9961 gtatcgccgc aatctgcaga aacaatatgt acaggcgtta agcgtgctga tcaatcccga
    10021 gccgccggtg cagatatcgc tgggcaacct gccccgcggg ctcgtcttct tcacgggcga
    10081 catccggttt acggatgtgc cttcgatagc gcgggcgcag ctggtggcgt tgcgcacgga
    10141 gatcggcgcg gccattgcgc gtgagggtga taaggtgagc aggtatcacc tgcaggatgt
    10201 gtcggaaagg atcagccagg cgttgaatcc gaagaaataa gaaaccaaac ctaaggcccc
    10261 cgcttcgcgg gggccttttc ttgcacgaag gcgccgcggc ggggttgggg gacggggctg
    10321 ttttcccctt tgagcgcgtg ccagatcacc gcattcatca gcctatcggg gatccggtcg
    10381 ggatcggaga gattgaaatg ccgcgaaatt tgcgcggacg ggccctctcc ggcgttacgg
    10441 agtttgatat ccaccccggc gggcaaggca tggaaggggg tcgtatcgcg cgtggaggtg
    10501 aagctgctgt aaagtggtgt cgaggcggcg tcgaactggc tcatcggcgg cagcccgagt
    10561 atcagctcca tcgtccgaag gatcgaggtc gtggaataca gcgtatggtc gacggagtga
    10621 cggcgcaccc atgggcttat gacataggcg ggcgagcggt gtgcatccac gtgatcggga
    10681 ccgtcctgcg catcgtcttc gagcacgaag atggccgact ccccccagat cgggctatgc
    10741 gaaatatgat ccaccagccg gccaagcgca aggtcattgt ctgccacctg ggccaccggc
    10801 gtataggatc ccttgttcaa tccgctggta tgatcattgg gcagatagac cgtgctgaaa
    10861 tgcgggacag cgccgatcgc caccagcgag tcgaaatcat gttcgaatac ggattcccgg
    10921 tagatatcct gaatatccag gttccagccc ggatatttaa agcaatagtt ggcggggatc
    10981 ttcagcaccg gcaatgtcgg atgcccgtcg tccatgaact cgccgtaatt acgaaagctc
    11041 acgcctgcac gggcgcaata gtcccagatg aacccattgg ttggattggc cacggcccgg
    11101 tggccatcga aatcatagtc cccgcccctg ccgctgtaat tcgtgggcca ggtcttttcc
    11161 gtaaaatccg tcgcatatcc cgccatcgac caattgtgac cgtccgcgct gacctcggca
    11221 tcgacataga aattatccag caggacgaag tcttcgacga tggcatgggc attcggcgtg
    11281 atcgtcttcc cgaaaagcac caggctggtg tctccattac cgcgcgggtc gtcccccaat
    11341 acctggtcat aggtcctgtt ctccttcagt acataaaaga cgtatctgat cggggatggc
    11401 tcgccggcgc ttcgcgggat cgggttgtgt tcctggcccg gcgcggtctg ctcgcgggta
    11461 cgcgtatata atgaattcgc atacacctgt tgtgtatagg cggacagttc ggccgccgaa
    11521 ggggtgggta tcgccgacag cgtgcccttg aacatgtttc cgatatactg ctcgtcatgt
    11581 ccatgcgatt ttttgaaggc gtcgccgtgt tccagcgggc tcggcccata cggatcgggc
    11641 atcgacgtaa aacccttccc attggcgacc agcagcgtcc ccgccgacat ccggacacag
    11701 gtgggatacc atcccaccgg aataaaaccc agtgaccggc tatggcccgg ctcggaaaca
    11761 tcgaagacgg cgagataatt gttgtcggcg ttggcgatat acagcgtatg atcttgggca
    11821 tccagcgcca cgctattggt ggtgctaccg atgggggctt cgggagataa tgccgtatgc
    11881 aaagtctcga gcacgcgccg gtgcgcgaca tcgatgaccg atacggaatt cgagttggcg
    11941 ttggctacat acagcatccg gccgtgccga tcgagcgtca tatccgtagg atgatcctcc
    12001 gtcgccaccg aatcccgcaa cctgtcctgt ttggtatcat agatccagat acggcgcgcg
    12061 ccccaggcgg cgatatacaa ttccttcccg gtgggcgata acacacaggt ataagcctcg
    12121 gcgccgagcg gggtccgcat cagaagcctc ttcgttcttg tatcgaagac atatagggcg
    12181 tcattctctt tggtgacgac gtagaggcgg tcacgtgttt cgtcgagaca gagaccggcg
    12241 gggctgatat tagcggccgg ccagggtttg cccaatacca gggtatcggg cctgacaagg
    12301 gtatcgccgc tgagggtaaa tcggatgatc gtattgtcat ttcctcccga cgcatagagc
    12361 cagggatgac gccttgcgaa ggccagcccg agccatgccc tgttcatcgg cacggatgcc
    12421 gtcacacgcc gggtccgcag atctatcaga tcgatggcct gggggccgtc gccattattg
    12481 gtgacgacgg cgtggacccc gtcgggagag agcgcgatat tcagcgggag atcggtgctg
    12541 agcggaagac ccgtaccggc gggcgtgagg aaccagccat tgggaagctg tgtgcgctgg
    12601 gcgttcagct ggcagcaaaa cagcaccgct gaggcaagaa ttgggagttt catagaataa
    12661 agataagggc ccccgctttg cgggggcccc atagaccatg attttattat tattctaacc
    12721 catatttcgt catataccga tgatacagct gcgtctgttt gttctcgagg ctgacttcgc
    12781 gcccctggat gaagcaatgt tcgatcagac tcgtcctcat gtcaaggatg tccccgccgc
    12841 tgatgacgat atttgcatcc ttacccggct ccagactacc cgtgcggtca tcgatcccgc
    12901 aaatcttcgc ggtattgagg gtaatggcct gcaaggcctg ctctttggtc agaccatagg
    12961 cggcggcagt acccgcattg aacgccaggt tgcgatacct ggattcttca tgattgtcat
    13021 tcagggagaa caggacaccg gctttttgca atacggccgg cgttttgaaa ggctggtcga
    13081 catcatcgtc ctccgtagtc ggcagtgcat gcagctgttg caggatcacc gagatattgt
    13141 tcttcttcag cagatcggcg atcaggaagc tctcgcttcc acccacgatc gtcacgtcca
    13201 taccgaactc ctttgcaaaa tcgatggcta ccagcatctg ccggacctgg tcgccatgta
    13261 cgaacagctt ctgggtatgg ttgaacaaac ctttcaccgc ctcgaatttc agattggtct
    13321 ccttatgccc gggctcggcc gcataggctt tcgcttcgct aaaaaaggcc ttcagctcgt
    13381 cgatcttcgc cagagcctct ttggtcggat caccggcctg ctgcccgaag gcaaaacgac
    13441 ctccgccacc accgccaaat cggcggggac gattcatgaa gctcggaaga ttgacatgga
    13501 tcgcattctc catcttatag gccgcgtctt cccagttcca tgcgtccagc tgcacgatcg
    13561 tcgacgagcc gctgatcgta cctccttcgg gtgtcacgcc ggcgagcagg atgccattgg
    13621 cgcgcaacgt gccgatgatc ttagaatccg tgttataggc gacgatagac ctgacgctcg
    13681 ggttcagatc gcccagctca agatagtcgt tcgatccgcg caccccgttg cctatttcct
    13741 tcagacccag gtcggtcacg ggcaatatca gccccggata gatctgtttg cctttggcgt
    13801 ctacgacctt cgcgccttcg gtattcggag gagcctgagc gccaacgctg acgatctttc
    13861 ctttttcaac gacgatggtg ccgtcttcga tgacctggcc gttaccgaca tggatcgtcc
    13921 cgccagtgat gatcagtttc ccggcataag gcttcgccgg gtatacatcg tcctgcgcca
    13981 tcgcagttgc ggcggacagg agcgtgcaaa aaagtaattt atatgatagt ttcatttgtg
    14041 ctgtttttag ttggttccgt tggtatcatc gtcgccatcg ctgacgtcga tagttaataa
    14101 cccgtcatga tggtcgtgat cgccgcagct aagctcgacg cgatagcttg gcatggcctg
    14161 cgccatcggc gttccggtcc ttttctcagc aaccagcttt tgaacgagcc tgttgcgctc
    14221 cgttgcgata tatttccgca tccgggcgtc gtggtcaaca tcgtaataaa cgatcccatc
    14281 cacgatagtc ttctcagccc ttgcataaat actcaagggg ttatcactcc acaggaccag
    14341 gtctgcatcc ttcccttccc tgatgctacc gacgcgatcg gcgacgtgca gggctttggc
    14401 gggattgagg gtcaccattt tgaacgcctc ttcctccgaa aggccaccgt attttacgct
    14461 tttggcggcc tcctggttga gccggcgggc ctgttcggcg tcgtcggaat tgatacaaac
    14521 attgagacct tctttcgtca tgatcgccgc gttgtaggcg atggcgtcct ggacctccat
    14581 cttgtaggcc caccagtcgg agaaggtgga agcattgctg ccgtgcgcct tcatcttgtc
    14641 ggcgactttg tatccttcga ggatgtgcgt gaacgtattg acggtgaagc cgaatttatc
    14701 ggcgacgcgc atcagcgccg tgatctcgct ttgtacatag gagtgacagg taatgaacct
    14761 tttgtggttg agtatctcga ccagcgcgtc cagctcgagg tcgcggcgcg ttgcaccggg
    14821 agccgccttg cgtgccttct cataatcgcg ggcgcgggta aatgcgtcca tgagcacctc
    14881 ttccacgccc atccgggtgt cggggaagcg gttgttattg gtggaggtgg tcctttttac
    14941 gttttctccg agggcgaact tgatgaaggg gtcccaatta cggaacttga gcgccgtatc
    15001 gttggcgccc cagcgcaatt tgatcagctg ggtctgaccg ccgatggtat tggccgaccc
    15061 gtggaggata tgcatgctcg taacgccacc ggccagacaa cgatagatac tgatgtcctc
    15121 ggggttaagg ttatcggcga tgcggacttc agacgttacc gattgcgcgc cttcattgat
    15181 ggagaaagct gcgatatgcg agtgttcgtc gatgataccc ggagccagcc atttgccgct
    15241 gccgtcgatg acctgtgcgt caccggctga taagtccttg cctatcttag ctatcttgcc
    15301 atttttgacc agcacatcgg tattcgccag acgtccttct ttttcattgg tccatacggt
    15361 agcattgcgg atcagcaggt catgcgccgt aggcaggcta tcccagccat agccgtcgaa
    15421 gggatagagc accttgccga ggcgttgctg gcgacgacgg ggtgtgctgc cggaatcggg
    15481 cgccagcgcc cttgtaaagg aggccgtcca gacgaccagt cctccggcgg aatcgacgcc
    15541 gttaccgttg aaaatatcgc cattgagcgt gcccgtgaga tgggttgcgg ctccgggacg
    15601 accgccgcca aagcttctgc ccctgggagc agcttcgccg ggagttcggg gagccggtgt
    15661 ggcgccggta gccccaccga atgtcaatgc gaccagcttg ccgtcgaagc taaactttcc
    15721 cgacagggta tctttgccga tgacggatgc cgaagtcggc gatttgacat cgagcacata
    15781 ggtcgacgat gcgcccgaag gcgaagtgat aacgagattg tactgccctc tgacgtcctt
    15841 ccatgcctct tctttcaccc catatttcct tccctgtacc cagttttcga cgatgcttgt
    15901 cttttcggaa aatatcggac cattggtgat caggaaattc gccagcttgc ctgcatccag
    15961 cgaacccacc ttatcataga tgccgatcag cgtcgccggc gtcttcgtca gcgcttcgag
    16021 cgcgcgggct tccgacaacc cgttgtcgaa ggccttccgg agattggtaa agaactgccg
    16081 cggatcgcgc agatcggagg ccgtcagaca gaacgtaatg cctgcctttt cgaatgcgcc
    16141 gggattggtc ggcgccagct cccaatgttt catatccgcg agggacacaa atcgggctgc
    16201 gtccggatcc tccacgtcca tagcctgcgg gaagttcacg gggacgatat aagccgcctt
    16261 cgtcgcagca atctcgtgca tccgctgata ttcattctcg ccggctttaa tgatatattg
    16321 tacgccgaac tcatcgccga tacggtcgcc atggagatcg ccccatttgt cgtcgccgtc
    16381 gaatatctgt ggcagcgtct gttcgtcatt gaaggcctga aggctgagat tggttccttc
    16441 atgatagttc tgattcttat accaggacgc atcgagatag gtctgacgga gcaacgcgat
    16501 ggaacccatt aaggaagaag gatagctctg cgtcgatgtt cctttattca gcgagtaatt
    16561 ggcggaagcg cgttgtttaa cgatgacgag gttctcgttt tgattgccca gcgtcaccac
    16621 gacgccggta cccctgacga taccgtcctt cttttgtgta agaacggtgc cgaagccgat
    16681 atcgcgaagc ggtttggccg ctgtttcatt gacaacgaag atgtgcgaag cgtccgtctc
    16741 ggggcgtacg gcctgattcc agttgtatgc accctttgta gcggacccga tctgggccgg
    16801 ctgtctgaaa aaggctgccg gatcgaatgc ggcgccccga ccaccacgtc ccggaacctc
    16861 cggttcggcg ataccatagt cgctgtacaa gtcgataaag gaaggataaa tatatttccc
    16921 gctgcaatcg acgaccactg catccttcgg cggcgtgaga cccgtgcctg cgccaacgat
    16981 cgtcccgtcg cggatgataa gcgtgccgtc gctgatcgtg ttgccctcat ccttcaccag
    17041 ggtggcatgg gtgaaggcat aacaacctac cctgggatcg gcaaccccat tgaccggaaa
    17101 ggtggattgc gcggatgtga acagcgtgag gaagagtagt gcggttgaga ggtaaaggat
    17161 cgtgcccctc ggtcggtggc gagcgtttct catgtttgtt agttttagcc ggatgaaata
    17221 atagtcccta atatacttaa atggagtaat tttaaagttc gaatatttta ttatcggtaa
    17281 aaagcaaaca atctatcggt gagtgagctg gcgcattata tcgatcacac catattaaaa
    17341 ccgaccacca cacatattga cattaataaa atatgtcagg aaacgcgcga gtatgggttt
    17401 gctgcagctt gtgttccgcc gtctttcgtg cgtttggccc ggcaggccgg tgtcagggta
    17461 gcgacggtca tcggatttcc cttcggttac tcgtttacga gggccaaact cgcggagata
    17521 gagcaggctt tatcggatgg ggccgatgag ctggacgtgg tcatcaacct gactgcgctg
    17581 aaggatgggg actggggcta cctggaaacc gaaatgcgtc tccttatgga gcctatccat
    17641 accgaagggc ggctgttgaa ggtgatcatc gaaagcggcg tgcttaccga agccgagatc
    17701 gtccggtgtt gcgagatcta tggaggaatg ggcgtggatt ttgtaaagac ttccaccggc
    17761 tatgcggagc agggtgcgac cctcggggcg gtgaggctta tgcgggcgca acttccggcg
    17821 tccgtgcaga tcaaggcttc gggagggatc cggacggccg cgctggcgca acaatttata
    17881 gaagcagggg cgtcgcggct tggtaccagc tccggcgtaa cactaataaa atcttatgta
    17941 taagagctta attgtattac tgttagtcgc gggtcgtgca gcggctcaaa cggattccgg
    18001 cggcgtcaac gtaacggtcg atccgcgtgt cgaccagctg ataaaaaaac aaatttccat
    18061 caacgaggcg accaccctgg aagcccgccg gcatgtcccg ggattccgga tacaggtgat
    18121 caatacgccc gaccgcaata aggtgaatgc ggcgaagatc aaggtctatg agcagtttcc
    18181 cgattggagg ccctatctca tttatcaggc gcctaactac aagttgaggg tgggcgattt
    18241 caagaccgag gacgaagcca atgcggcaat gcagcagtta tcgaggcttt ttcccagcgg
    18301 cttatacgtg atacgcgata tcatagaggt gaagctatcc gacgttcaga aaatggattc
    18361 gacgaacaat cgctaacgaa agcgcactat catgctgaaa gacacgatcc ggcagctggc
    18421 caaaatatat tcccccgacc tcatcgccat ccggcatcac ctgcatgcgc atcccgaatt
    18481 gtcttacaaa gaatatgaga cttcggcgtt tgtgcagcgg caattggagg ttctcggaat
    18541 accgtttcgg attatggctg gtacgggtgt ggtggggttg ctggaaggac gtaatccaaa
    18601 gagccgggtg atcgcactga gggccgatat ggatgccctg ccgatccggg aggaaaataa
    18661 ggtcgaatat cgtagcaaga atgaaggtgt catgcatgcc tgcgggcatg acgtgcatac
    18721 gacagtgctc ctcggcgcgg cccggatcct aaacgacctg aagggcgatt gggagggaac
    18781 cataaaactc atcttccagc ctggcgagga aaaaaatccg ggtggcgcca gcctcttgat
    18841 caaggaaggg gtattgaaag accccgagcc ccaggccatt ttcggattgc atgtgcatcc
    18901 gggtctggaa accggaaagc tcagctttcg gggcggaatg gtcatggcca gcgccgacga
    18961 gctgtatatc accattaagg gtaaaggagg gcacgccgct gccccacacc agacggtaga
    19021 caccatttta gtcgcctccc agctggtcgt tgccctgcaa cagatcatta gccggaatcg
    19081 aaatcctctg tccccctccg tattatcgat cacctctttc cagggagggc atacgaccaa
    19141 tgtcatcccc agcgaggtga agctgatggg taccttccgg gccatggatg aggagtggcg
    19201 tttccgcgcc cacgagctga tccggcagct ggctacccag ctggtccata gcatgggagc
    19261 agagatcgat ctgcatatcg acgtgggtta cccgatggtc tataataatg aagcgctgga
    19321 ccgcgtggca aggacggcag cacgcgaata catgggggcg gatcatgtgc tggaaaccga
    19381 agtccgcatg ggcgccgagg attttggcta ttatacacgc ctggtcccgg gctgcttcta
    19441 ccgcctcggc gtaatgaatg tcgctaaagg gatcacctcc ggggtacata cacccacctt
    19501 cgacatcgat gaatccgcca tagagattgg agcgggcatg atggcctggc ttggagcgaa
    19561 tattcggttc gaacaatgat taccagccat tttcgtattt tgcaccgata atgacgcaga
    19621 aaaaacagct ccgcaaggag caggcgctcg aataccattc caaaggaaga cccggcaaga
    19681 tcgaagtcat tccgaccaag gaggcaaaaa cacagcgcga tctgtccctg gcctattcac
    19741 ccggtgttgc ggaaccctgc aaggagatcc ataagaacgt cgaggaagtc tataaatata
    19801 ccgccaaggg caacctggtg gccgtcatca gcaacgggac ggctgttttg ggtcttggtg
    19861 atatcgggcc ggaggcctcc aaacccgtca tggaaggcaa gggggtgcta tttaagatct
    19921 ttgccgacat cgacgtcttc gacatcgaga tcaatgaaaa agaccccgaa cgttttgtac
    19981 agattgtcaa agcattggaa cccacctttg ggggcatcaa cctggaagac atcaaagccc
    20041 cggaatgttt ctatatcgag cggtcccttc gcgagcaatt gaagataccg gtgatgcatg
    20101 acgaccagca cgggacggcc atcatcagca gcgccgccct cctgaatgcg ctcgagctcc
    20161 agaaaaagaa gatcgataaa gtgcgtttcg tcatcaacgg tgccggagcc gccgccatgg
    20221 cctgcgtcaa gttgtatgtc gccctgggcg ccaaacccga gaatttcacc cttttcgata
    20281 aggacggcgt tctccacgag gaccggccgg atctcgacga ggataaaaag gtttttgcag
    20341 taaaaaaggc cgatctgacc ctcgacaagg ccttaaagga cgcagatgtc ttcgtcggtc
    20401 tcagcgtcgg caatgtcgtg acggccgaga tggtcaaaag catggcgcgc aaacccatcg
    20461 tattcgccat ggccaatccc gatccggaaa tagcctatga catcgcgacc gcatcgcgcg
    20521 aagatatcat catggccacg gggcgcagcg attatcccaa ccaggtgaac aatgtactcg
    20581 ggttcccctt catcttccgc ggcgccctcg atgttcgcgc cacgcagatc aatgaggcca
    20641 tgaagcttgc cgcggtaaag gccctggcgg atctggccaa gacccccgtg ccggacatcg
    20701 taaacatggc ttacaatgaa aagaacctct cattcagcgc cacctatatc atccccaaac
    20761 ctttggaccc gcggcttttg tcgaccgtag ccccggccgt ggcgcgcgcg gccatcgaga
    20821 gcggcgtagc gcaacaccag atcgacaact gggaggctta taccatcgag ctgaaccgtc
    20881 gtctcggcct tgataaccag ctcatgcgcg tcatgagcag caaggcgcgg ctgaatccca
    20941 aacgggtggt cttcgcggag gcggataaca tcaagatcct aaaggtggcg caactgctgt
    21001 atgacgaagg ggtcgccttc cccatgttgc tgggcgacga gcggaaaata cgtcatatcg
    21061 catcgatgaa ccagatcgac ctggaaggca tgcccgtcat caatccttac aacgacgaat
    21121 atgaggaaca gcgcaaaaag ttcggcgagc tgttcttcca aaaacgccag cgacgcggct
    21181 tcaataaata tgaagcctac aagaccatgc gcaacagaaa ttattttgga tgcatgatgg
    21241 tcgagacggg tgaggcggat gccatgatct ccggtctgac caagaactac cctgacacga
    21301 tcaagcccgc cttacaggcc atcggcacgg aggaaggggt caacaagatc gccgggatgt
    21361 acctgttgct gaccaaacgg ggcccgttgt tcctcgccga tacgacggta aatttcaatc
    21421 cgacgacgga agagctggcg gatatcaccc tgttggtcgc ccgcgaagta caaaatttca
    21481 acctgacccc gcgggtcgcg atgctcagct attccaattt cggcaccagc acgtcacccg
    21541 aggccaaact ggtagcggat gcacgggaga tcgtcaaacg gaagaacccg cagctgatcg
    21601 tcgatggcga aatgcaggcc agcgtggcgt tgaacaatga catcctccgc gagaactacc
    21661 cgttcagcga gctggtcgac caggaggtaa atacgctcgt cttccccaat ctcgccgcag
    21721 gcaacatcgc ctacaatctg cttaaagaag taggcggcgc cgacgccatc ggccccatat
    21781 tgcttggtct gaagaaacct gtgcatgtcc ttcagctggg aagtacggtc cggcagatct
    21841 ataatatggc tctggtagcc attgtggatg cacagattaa atgcaagaac aatgcgacgg
    21901 acgaagaggc gaaaaaggcg ccatggtgga agcgtaccag aaactaatat ataccgcatg
    21961 aattttttta ctgaattcgg gagttatctg cttacactaa agggcatgtt caccaagccg
    22021 gagaacggca agatgtattg gaaagaattc atgcatcagt gttccgagat cgggatcggc
    22081 agtctgggta tcgtcgccat catctccatg ttcatgggtg cggtttccac cttgcagacg
    22141 gcctatcagc tggtcagtcc gctgataccc ttgtcgacca tcgcacagat cgtacgcgat
    22201 acggtcatcc tggaattctc gccgacgctg gtgtgcatcg tgctggcggg agtcgtggga
    22261 agcaagatcg cgagcgagct ggggaatatg cgtgtctccg agcagatcga tgcgctggag
    22321 atcatgggca tcaataccaa gacctatctc atcctgccca aattactggc cgcgatcatg
    22381 gtgatcccca tgctggtcgt gctgtcggca gtgctgggca tctggggcgg aaggctggcg
    22441 ggcacactaa cgggcatcct gtcgaaggaa atgttcgaca agggtcttca ccagtatttc
    22501 aaaccttata atgttttttt cgccatggca aaggcgtata cctttgcgtt catcatctcc
    22561 agcataccgg cttattacgg atataacgtt tccggcgggg cgctggagat cggacgggcc
    22621 agcacgagat cggtggtggt gagttgtatt ttggttttgt ttgcagacta tcttctttcg
    22681 gcacttttgc tctaagatgg aaaggtcgcg atcggccagc cgaaagaaga taaaaaatca
    22741 ttagccccgc ggaggccggc gaagacggcc aatgccgagc ggagactgag aatgaggatc
    22801 aaagtccaat agggcgctgc tgttgaacgg agcgtcgcaa cgatgctcaa ccagggcgat
    22861 accgccgata acaaagaaga aaatgatcga gatcaaaaat atcaagaaag ggttcggtga
    22921 gaagtccgtc atcaatgatg tgagcaccgt ctttaagacc ggcaaatgca atctcatcat
    22981 cggcaccagc ggcagcggca agaccgtcat gatgaaatgc ctggtcgggc tgatggagcc
    23041 ggacagcggc gagatcattt acgacggcca aaacttctcc gagatgaatg aagaggggcg
    23101 gaaggagctt cggcaaagca tcgggatgtt gttccagggt tccgccttgt tcgacagcat
    23161 gaccgtagag cagaacatca tgttcccgct ggacatgttc accaaaaata cttttgccga
    23221 aaagttgcga cgcgtgaacg aggtactcga cagggtgaac ctgcagggcg ccaataagaa
    23281 attccccgcc gagatctcgg gtgggatgaa aaaaagggtg ggcatcgcgc gggcgatcgt
    23341 gctcaaccct aaatacctgt tctgcgacga acccaacagc ggcctggacc cgcagaccag
    23401 tctcctgatc gacaagctga tcaaagagat cacgctcgaa tataacatca ccaccgtggt
    23461 caacacccat gacatgaaca gtgtcatgga ggtcggggac catatcatgt atatgcacca
    23521 gggggtcaag gagtgggaag gaaccaacaa ggagatcata tttagcaaga ataagttgct
    23581 aaatgacttt atttttgcgt cggaattcct gcgtgacgcc aaggatatgc ggatgctgga
    23641 ggtcagggga aagatcgata acgaccggaa catggatgag ttattaaaat aaattttcaa
    23701 ttgtcatgtc aatcttagtt aacaagcatt ctaaaatcat cgtacaaggc tttacaggaa
    23761 cggaagggac atttcacgca acacaaatga tcgattacgg cacgcaggtg gtaggtggcg
    23821 ttacccccgg caagggtggc tccacgcata tcgagcggcc ggtcttcaat acggtggagg
    23881 cggccgtaaa ggccaccggc gccgacgttt ccatcatatt cgtacccccc gcattcgctg
    23941 ccgacgcgat catggaggcg gcagaagccg gtatcggact ggtcatctgt atcacggaag
    24001 gcatcccggt acaggatatg gtgaaggcaa agaattatct gctcgggaag aatacgcgtc
    24061 tgatcggtcc caattgtccc ggggtcatca ccgcgggaga atgtaaggtt ggcatcatgc
    24121 ccggtttcgt tttcaagacc ggaaagatcg gcatcgtcag taaatcgggc accctgacct
    24181 atgaggctgc ggaccaggtg gcgaaggccg gtctcggtat ctccaccgcg gtaggcatcg
    24241 gcggcgaccc catcatcggc acgccgacca aagaggcggt cgaactactg atgaatgacc
    24301 ccggaaccga aggtattgtc atgatcggtg agatcggagg gagcatggag gcagaagcgg
    24361 cccgttggat ctctcagaat aaccggaagc cggtggtggg atttatcgcc gggcagacgg
    24421 cgcctcccgg acgtcgtatg ggtcatgccg gtgcgatcat cggcggtgcg gatgatactg
    24481 ccgccgcgaa gatgaagatc atggccgaat gcgggatcac ggtcgtgcag acgccggcgg
    24541 agatcgggtc gacgatggcg aaggtgttga
//
LOCUS       DASYSV010000037         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1930576, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000037 DASYSV010000000
VERSION     DASYSV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930576"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VGQ51_01135"
     CDS             complement(<1..370)
                     /locus_tag="VGQ51_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035184.1"
                     /translation="MLRPIAIYLLLQLSTGYAPAQLHWKRIDSAYGPLPSSMHVYRSA
                     DSLDGFPSIAYYVTARLKDRRLQFTTATGQGKRFTPDQYYQLEQSPYLVVNCAYFSGN
                     QNLSLVVQDGRMLAYNVTSLR"
     gene            complement(385..1311)
                     /gene="lipA"
                     /locus_tag="VGQ51_01140"
     CDS             complement(385..1311)
                     /gene="lipA"
                     /locus_tag="VGQ51_01140"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEV9035185.1"
                     /translation="MDNFGNSPEAQSKIQKPNWLRVKLPIGENYKHVRGLVDTHKLHT
                     ICESGNCPNMGECWGEGTATFMILGNVCTRSCGFCAVATGRPDPVDWDEPQRVAEAIH
                     LMKVKHAVITSVDRDELKDGGSIIWQNTIRAVKALNPGTTLETLIPDFKAEKDNIARI
                     IEAAPEVVSHNIETVERLTRQVRIQAKYWRSMETLAVLKEGGMRTKSGIMLGLGERKE
                     EVIQTMTDLRNSGVDVITLGQYLQPTKQHLPVVRFVHPDEFAELREIGYNLGLDYVES
                     GPLVRSSYHSERHVIPGYGRTKWLKEKEASES"
     gene            complement(1326..2159)
                     /locus_tag="VGQ51_01145"
     CDS             complement(1326..2159)
                     /locus_tag="VGQ51_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035186.1"
                     /translation="MKKLKPLRHILLTALASTLILQLVAQNNPDSAAMMSKMNKQNAK
                     RQRMNNMLRMEEEGDLIFNKHSIFGIRLATDGYGISYEKGKFKTPSKTILYEFELNEK
                     HDPKEHHISATSDGINFSSVVPYKLNNLYEFKMAIGQQHLLGGKGNKNGVAVTYLYAG
                     GVTIGLLKPYLLDIQNQITGATTRKTFAQMATDTTEGDYITGASGFTVGWDKLSIKPG
                     INAKQALRFDYGRLNQTITAIECGLTEEYYFSKIPLVYLVKEHAFFFNAYVSILFGSR
                     K"
     gene            2211..2642
                     /locus_tag="VGQ51_01150"
     CDS             2211..2642
                     /locus_tag="VGQ51_01150"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV9035187.1"
                     /translation="MTSTVIYEGDLRTVARHLQSGTEIQTDAPTDNQGKGERFSPTDL
                     VATALGACMATTMGIKSRDMQVDLRGMKLSVQKIMKPDPRRIAGIHIVFEFPAGLTVD
                     EKQRVILERTANTCPVIYSLHPDMEVKVEFNWNSAVAAPAH"
     gene            complement(2605..>2996)
                     /locus_tag="VGQ51_01155"
     CDS             complement(2605..>2996)
                     /locus_tag="VGQ51_01155"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEV9035188.1"
                     /translation="MATGWFTFIRETVSSKELLRRLEGHPGLGADMQRGLVINAFIPL
                     VFAYGWLRDEPASREKALRWLRELRPEKNIILSRWKLLGMTPRNAGDSQALLQLKKEY
                     CDTRRCLECAIGRALINGPGQPPRCSS"
BASE COUNT          652 a          786 c          822 g          736 t
ORIGIN      
        1 cgcgaagcga tgtgacgtta tatgccagca tccggccgtc ctgtaccacg aggcttaggt
       61 tttggttccc cgagaaatag gcgcagttca cgaccaggta aggcgactgc tccaactgat
      121 aatactgatc gggggtaaat cgtttgccct gccccgttgc agtggtaaac tgcaatcgtc
      181 ggtccttaag ccgcgcggtg acgtaatagg caatcgaagg aaaaccgtcg aggctgtcgg
      241 ctgaccggta gacgtgcatc gaagaaggaa ggggaccgta tgccgaatcg atccgtttcc
      301 aatgcagttg tgccggagca taaccggtcg atagctgcag caaaagatag atggcaatgg
      361 gtcgtagcat cggggcggac atttttacga ctctgaggct tccttctcct tcagccattt
      421 cgtccttcca tagcctggga taacgtgccg ttcggaatga taggaggatc gtacaagggg
      481 accactttct acatagtcga gtccaagatt atagccgatc tcgcggagtt cggcaaattc
      541 atcgggatgg acgaacctga ccaccggcaa atgttgtttg gtaggttgga gatactggcc
      601 cagtgtgatc acatcgacgc cgctgttgcg caggtccgtc atggtctgaa tgacctcctc
      661 ttttctttcg cccaaaccga gcatgatccc actcttcgtg cgcatacctc cctccttcag
      721 cacggccagc gtttccatgc tccgccagta tttggcctgg atgcggacct gtctcgtgag
      781 ccgctcgacc gtctcgatat tatgcgagac gacctccgga gcggcctcga tgattcgggc
      841 gatattgtct ttttccgcct tgaaatcggg gatcagcgtc tccagcgtgg tgccgggatt
      901 gagggccttc acggcccgga tggtattttg ccatatgatg ctgccgccat ccttcagctc
      961 gtcgcgatcg acggaggtga tcacggcatg tttcaccttc atgagatgaa tggcttcggc
     1021 tacccgctgc ggttcatccc agtctacggg atcgggcctg ccggtggcga cggcgcagaa
     1081 gccgcagctc cgtgtacaaa cgtttccaag gatcataaaa gtagccgtgc cttctcccca
     1141 gcattccccc atattcgggc aattaccgct ttcacagatg gtgtgcagtt tatgggtgtc
     1201 tacgagaccg cggacatgct tatagttctc gcctatcggc aactttacgc gtaaccaatt
     1261 gggcttctgt attttagatt gcgcctccgg gctattgcca aaattatcca tttaacaaaa
     1321 gtaactcatt ttcgactgcc gaagaggatg ctcacatagg cattgaagaa gaacgcatgt
     1381 tctttgacca ggtagacgag agggatcttg ctgaaataat attcttccgt cagaccgcat
     1441 tcgatagccg tgatcgtctg attcagacgc ccgtagtcga atcgtaatgc ctgtttggcg
     1501 ttgatgccgg gtttgatgga gagcttatcc cagcccacgg taaaaccgga tgccccggtg
     1561 atataatcgc cttctgtcgt atcggtcgcc atttgggcga atgtctttct tgtcgtggcg
     1621 ccggtgatct ggttctggat atcgagcaga tagggtttca gcagaccgat ggtgacgccg
     1681 cctgcataga gataggtcac ggcgacgcca tttttgttgc ccttcccacc gaggaggtgc
     1741 tgctgaccga tggccatctt gaattcgtat aggttattca gtttgtaggg aaccaccgag
     1801 gaaaaattga tgccatcgga ggttgccgag atatgatgct ctttcggatc gtgtttttca
     1861 ttgagctcga attcatacag gatggttttg ctgggcgtct tgaatttgcc tttctcatag
     1921 gagatgccat agccgtccgt cgccagccgg ataccgaaga tgctgtgttt attgaagatg
     1981 agatcgcctt cctcctccat gcggagcata ttgttcatcc gctgccgctt ggcgttctgc
     2041 ttgttcatct tggacatcat agccgcgctg tcgggattgt tctgggccac cagctgcagg
     2101 atcagggtcg aagccaatgc ggtaagaagg atgtgcctga ggggctttag tttcttcact
     2161 gacgatgatt taaattaact ggcgtaaatt tatgcaaaat ttgttgcaca atgacgtcca
     2221 cagtaattta cgaaggggat ttacgaacgg ttgcccggca cctgcaatcc ggcaccgaaa
     2281 tacagacaga tgcgcctacc gataaccagg gaaaggggga gagattctct cctaccgatc
     2341 tggtcgccac tgcccttggg gcctgtatgg ccacgaccat ggggatcaag tcccgcgata
     2401 tgcaggtcga tctccggggc atgaagctgt ctgttcaaaa gatcatgaaa cccgatccca
     2461 ggcggatcgc cggcatccat atcgtattcg agttccccgc cgggctgacg gtagatgaaa
     2521 aacaacgggt gatcctggaa aggacggcga atacctgtcc ggtcatctat agcttgcatc
     2581 ccgacatgga agtgaaggtt gagttcaact ggaacagcgc ggtggctgcc ccggcccatt
     2641 gatcagggcc ctgccgatcg cgcactccag gcatcggcgg gtatcgcaat attccttttt
     2701 taactgcaga agcgcctgtg aatcgccggc attccggggt gtcatgccca gaagcttcca
     2761 ccgggagagt atgatattct tttcgggacg caattcccgt agccagcgca acgccttttc
     2821 ccgggatgcg ggctcatctc tcaaccagcc atacgcgaaa acgagtggga taaaggcatt
     2881 gatgaccaaa ccgcgttgca tatcggcgcc aagaccggga tgtccttcca gccgtcgcaa
     2941 cagctccttt gaagagaccg tctcgcggat aaaggtgaac caaccggtgg ccatca
//
LOCUS       DASYSV010000038         3998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2186105, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000038 DASYSV010000000
VERSION     DASYSV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186105"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGQ51_01160"
     CDS             <1..273
                     /locus_tag="VGQ51_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEV9035189.1"
                     /translation="YETVPPILQSLGKIKNPWPNVDAHSGALLVHYGLVEYEFYTVLF
                     GVSRALGVLASLCWDRALGFPLERPKSVTTELVKDWLKGEGEIWGE"
     gene            complement(437..1597)
                     /locus_tag="VGQ51_01165"
     CDS             complement(437..1597)
                     /locus_tag="VGQ51_01165"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEV9035190.1"
                     /translation="MSTHPITKAIRIRTGQTQEKEHSSPLYLTSSFCYDDAEEMRAVF
                     AEEKEAFIYSRFSNPNVQEFTDRLCALEGAEAGFATASGMSAIFASFMALLKKGDHLL
                     SSKSIFGSTHTVVTKYLPKYGIEYSYVDAADPASWEKAIRPNTKMFFLETPTNPGLDI
                     IDLEAAAALSRKHNLILNVDNCFATPIGQTPIQYGADLVTHSATKWLDGQGRVVGGAV
                     VGRKQLIHEIYLFCRATGPALSPFNAWVLSRSLETLDVRMERHSSNALFLAQSLENHP
                     AISALKYPMLPSHPQHAIAKKQMRNGGGILCFDLKGGLEAGRNFLDKLKLLTLTANLG
                     DTRSIASHPASTTHAKLTPEERAAVGITPGLIRISVGLEHRDDILQDILQAL"
     gene            complement(1602..2045)
                     /locus_tag="VGQ51_01170"
     CDS             complement(1602..2045)
                     /locus_tag="VGQ51_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV9035191.1"
                     /translation="MTRIKVKRVSGNFGFEAQDAAGHTVHMDSNPESGGQDFGVRPMQ
                     MLLMGIGGCSGIDIVSILGKQRQTVDAFYMTIEGDRQKGTEPSLWEYVHIRFDLEGAI
                     YPDKAIKACQLSMEKYCSVAATLREANCRITWEVYVNNTFAYSTK"
     gene            2209..3225
                     /gene="metX"
                     /locus_tag="VGQ51_01175"
     CDS             2209..3225
                     /gene="metX"
                     /locus_tag="VGQ51_01175"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEV9035192.1"
                     /translation="MNYFTYEHPFELEGGGCLPGITVAYHTYGRLDKDGGNVVWVCHA
                     LTANSDVAQWWPGVVGEKGPIHPDRYFIVCANILGSCYGSTGPLSTDSRTGEPFFAGF
                     PMITIRDMVNAHMLLRKHLGIKRIHLLMGGSMGGYQALEWSVMEKDVIERLFLIATSP
                     SESAWGIAVHTAQRLAIEADPTWCEPSPGAGSKGLKAARAIGILTYRNYGIMVEKQSD
                     ADTEKLDGFRASSYIDYQGDKLVSRFNAVCYWLLTKAMDSHQLARGRGGDVEVVLRTI
                     TQPTLLIGVSSDILCPLVEQRHMADFLPDAKLVEIDSAYGHDGFMVEGEKIATHLGAW
                     LEVR"
     gene            complement(3261..>3998)
                     /locus_tag="VGQ51_01180"
     CDS             complement(3261..>3998)
                     /locus_tag="VGQ51_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9035193.1"
                     /translation="SVTTTDLAAYPTTSDSIFQTALTIVPTVKSTEVKQLYSRKALSY
                     YRGGYLPTLTLYGSLNTNYYSLDLKETSVANVQTGLNTGYYVQGSNASVLYDKTNYNS
                     EKVSWGDQFTANKSPQIGLQLNIPILNNLRARNNVRTAKVNLKTAEFNNNTARLTLQQ
                     NVEQAFQYMLLAYKQYKFYTEQSDAYSESFRITNIKFGEGVVTADVYINSKQRADAAA
                     TNLAAARYAYIFRTKVLDYYQGRLAIP"
BASE COUNT          828 a          828 c         1379 g          963 t
ORIGIN      
        1 tatgagacgg tgccgccgat attgcagtcg ttgggaaaga tcaagaaccc atggcctaac
       61 gtggatgcac atagtggcgc gttgctggtg cattatgggc tggtggaata tgaattttac
      121 accgtcctgt tcggggtcag ccgtgcgctg ggggtattgg cgagtctttg ctgggaccgt
      181 gcgttgggat tcccgttgga gcggcccaag tcggtaacga ctgagctggt gaaggattgg
      241 ttgaagggag agggggagat ctggggggag taggcgtgcc tatgccatgt tgcgttcgtc
      301 gtgtcctgtg gaagccacaa gacaacaagc acctacaagt ttcttggcac cggtgattgg
      361 agatcacagc ggccgatggc gacgggtccc agaccgcaca agcccgggga aggaggcgtg
      421 gagccggagc atatggttag agcgcctgga ggatgtcctg caggatgtcg tcgcggtgtt
      481 cgagaccgac ggagatacgg atgaggccgg gggtgatgcc gacggcggcg cgttcttcgg
      541 gggtcagctt cgcgtgtgtc gtgctggcgg ggtgggaggc gatgctgcgt gtgtcgccta
      601 aattggcggt cagggtaagc agtttcagct tatcgagaaa attccggccg gcttcgagac
      661 cgcctttgag gtcgaaacag aggatgccgc cgccgttgcg catttgtttt ttagcgatgg
      721 catgctgcgg atggctcggg agcatgggat atttgagcgc cgagatcgcg ggatgatttt
      781 cgagcgactg ggcgaggaag agggcgttgg aggagtggcg ttccatacgg acgtcgaggg
      841 tttcgaggct gcggctaagg acccaggcgt tgaaagggga aagggcggga ccggtggcgc
      901 ggcaaaacag gtagatctca tgaatgagtt gtttgcgtcc cacgactgcg ccgcctacga
      961 cgcggccctg gccgtcgagc catttggtag cggaatgagt gacaagatcg gcgccgtatt
     1021 ggatcggcgt ttgtccgata ggggtagcga agcaattgtc tacattgagg atgaggttat
     1081 gtttgcggga gagtgcggct gcggcttcga gatcgatgat atcgaggccc gggttggtgg
     1141 gcgtctccag aaagaacatt ttcgtgttgg gtcggatggc tttctcccag gaagcgggat
     1201 cggcggcgtc gacatacgaa tattcgatgc cgtatttggg gagatatttg gtaacgacgg
     1261 tatgggtgct gccgaagatg gatttggagg acaggagatg gtcgcctttt ttgagcaggg
     1321 ccataaagga ggcaaagatg gcgctcatac cggaggcggt ggcaaaaccg gcttccgcgc
     1381 cttcgagagc gcagagacgg tcggtgaatt cctggacgtt gggattgctg aaccggctat
     1441 agatgaaggc ttctttttct tcggcgaaga cggcgcgcat ttcttcggcg tcgtcgtaac
     1501 agaagctgct ggtgaggtag agcggggagg aatgttcttt ttcctgtgtt tgcccggtgc
     1561 ggatgcggat ggcctttgtg ataggatgag tcgacatgta gttattttgt agagtaggcg
     1621 aaagtgttgt tgacatatac ctcccaggtg atcctgcaat tggcttcccg gagggtggcg
     1681 gcgacggagc aatatttttc catggatagc tggcaggctt ttatggcctt gtcgggatat
     1741 atggcgcctt cgagatcgaa acggatgtgg acatattccc agagggaggg ttctgttcct
     1801 ttttggcgat cgccttcgat ggtcatgtaa aaggcgtcta ccgtctgtct ttgtttgccg
     1861 aggatggata cgatgtctat gccgctgcat ccgccgatgc ccatcagcag catttgcatg
     1921 ggacggacgc cgaagtcctg accgccggat tcaggattgc tgtccatgtg aacggtgtgg
     1981 ccggccgcgt cctgggcctc gaacccgaag ttaccgctga cccttttcac cttaatcctt
     2041 gtcatttcta atttttgtgc aaaggtaagg ggggcggagg ggataagcaa aggaaaggaa
     2101 ggcgggggga taagcaaagg gctgtgaggc gtaggcgagg ggataatggg aaaaaaggta
     2161 aggtggaatg aaaggtatgc gcttattttg cagcgaaatc gagcgcggat gaattatttt
     2221 acttacgagc acccgtttga gctggaggga ggaggatgtt tgcccggtat tactgtagcc
     2281 tatcatacat atggccggct ggacaaggat ggcggcaatg tggtgtgggt gtgtcatgct
     2341 ctgaccgcca attccgatgt cgcgcaatgg tggccggggg ttgtggggga gaaggggccg
     2401 atacatccgg accggtattt tatcgtttgt gccaatatat tggggtcctg ttacggatcg
     2461 acggggccat taagtacgga tagccgtacc ggggagccgt ttttcgcggg ttttccgatg
     2521 atcactatcc gggacatggt gaatgcgcat atgctgctca ggaagcacct ggggatcaaa
     2581 cgtatccacc tgttgatggg cggaagcatg ggtggttacc aggcgctgga atggagcgtc
     2641 atggaaaagg acgtgatcga aaggttgttc ctcattgcga cctcaccttc cgagagtgcg
     2701 tggggaattg cggtacatac ggctcagcgg ctggctatcg aagccgatcc gacatggtgc
     2761 gaaccttcgc caggtgcggg gagcaagggg ttgaaagctg cgcgggcgat cggcatcctg
     2821 acctatcgta attatgggat catggtggag aagcaatcgg acgcggacac ggaaaaactg
     2881 gatgggttca gggcgtcttc ctatatagac taccaggggg acaaactggt gagccgtttc
     2941 aatgccgttt gttattggct gttgaccaag gcgatggaca gtcatcagct ggcacgcggg
     3001 cggggcgggg atgtagaggt agtgttgagg acgatcacgc agcctacgct gttgataggt
     3061 gtttcgagcg acatactttg tccgttggtg gagcagcggc atatggcgga ctttctaccc
     3121 gatgcgaagc tggtcgagat cgactcggcg tatgggcatg acgggtttat ggtggaaggg
     3181 gaaaagatag ccacgcattt gggtgcgtgg ctggaagtaa gataagggcg gcggatgggg
     3241 cgccgtgtgt ggctaaagac ttaggggatc gcgaggcgtc cctgataata atccagcact
     3301 tttgtcctga agatatatgc ataccgcgca gcggcaaggt tggttgcggc tgcgtctgcg
     3361 cgttgtttgg aattgatata cacatccgcg gtcaccacgc cttcgccgaa tttgatattg
     3421 gtgatgcgaa aggactcgga ataggcgtcg gactgttcgg tatagaattt atattgttta
     3481 taggccagga gcatgtactg gaaggcctgt tcaacgtttt gttgaagggt cagcctggcg
     3541 gtattattat tgaactcggc ggtcttcagg ttaactttcg ccgtccggac attgttgcgg
     3601 gccctcagat tgttgagaat ggggatgttg agctgtagtc cgatctgggg tgatttattg
     3661 gcggtgaact gatctcccca ggacactttt tccgaattgt aattcgtttt gtcatagagg
     3721 accgacgcat tggatccctg aacgtaataa ccggtgttca ggccggtctg gacgttggcg
     3781 acggaggttt ctttcagatc gaggctataa tagttggtat taaggcttcc gtaaagggtc
     3841 aacgtaggta aatagccgcc acgatagtag gagagggctt tccgggagta caattgcttt
     3901 acctcggtgg atttgacggt tggaacgatg gtcagtgccg tctggaagat gctatcggag
     3961 gtagtcggat aggccgccag atcggtcgta gtaacgct
//
LOCUS       DASYSV010000039         6765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2186182, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000039 DASYSV010000000
VERSION     DASYSV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186182"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1689
                     /locus_tag="VGQ51_01185"
     CDS             103..1689
                     /locus_tag="VGQ51_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEV9035194.1"
                     /translation="MEKRNAFRQLALLLLPALSIGLMADAQSAGSAGPQVHTIHGIIE
                     GVIGKSGVRSFKGIPYAAPPIGQLRWKEPQDGADWTGVRVCDRFGPRAMQPPIYSDMV
                     FRSAGMSEDCLYLNVWAPAGASPGGGRKLLPVLVYFYGGGFVAGDGSELRYDGESMAT
                     KGIVSVTVNYRLSIFGFFAHPDLTKESPHHSSGNYALLDQTAALRWVQQNIANFGGDP
                     RRVTIAGESAGSISVSALMATPLTKGMMAGAIGESGSLLGALPPVGVSQAEETAVQFT
                     GTVGATSLAELRAMPADSLLAAAMRFGPFRFHMTIDGYFFPKSPYDIYASGEQAHIPL
                     LVGWNSQEGDYHAILGNAAPTVANYEAAVRKAGGVFADTILRLYHASADAEVEGVATA
                     LASDRFIAFSTWRWADLQGKTGGQPVYRYFYERPRPATVAGEPAAKGAVHSAEIEYAM
                     GNLSTNKVFAWTSADYAVSSVLQGYFVNFIKKGDPNGAGLPHWPAANQGSVVSVQHIG
                     VKTRTEPEQHRDRYEWASKR"
     gene            1807..4038
                     /locus_tag="VGQ51_01190"
     CDS             1807..4038
                     /locus_tag="VGQ51_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEV9035195.1"
                     /translation="MNKILLTVICAAVTVTALAQETRVYTNADYERAESMLSYNTGPL
                     IDRADVRPTWVEGDRFWYRVLTATGSEYVLVDPLKKTRTAYPDREKLFAAISALTGKP
                     VEAAPREGGGRRGFRGEGGGNSVLSPDGKRAAFIRDYNLWVRDVATKQETQLTTDGVK
                     DFGYSTDNAGWKSSDRPVLRWSPDGKKIATFKDDQRNVGEMYLVSTKVGHPVLHAWKY
                     PLPGDSVIPMITRVIIDVDAPKVIPLNIPPDPHRATLSDDISSSGTFDDVDWGTDENQ
                     LAFVSTSRDHKDEKFRIANALTGEVRDVFEEKVATQFESGRGTIDWRYLQGTHEIIWY
                     SERDNWGHLYLYDASTGQLKNQITKGDWLVSRLVKVDEKKRVIYFMGAGKEPGCPYFN
                     YLYKIGFDGKHMVLLTPEKGNHTITFSPTGEYFVDTYSQPDVAPVSVLRNQEGKLVSD
                     LEKADISRLVASGWRPPIPITVKAHDGQTDLFGLLFVPSHLDSTKKYPIIDYIYPGPQ
                     GGSVGNWGFQASRNDNQALTELGFIVVEIEGTSNPYRSKSFHDMSYGNMAENTLPDQV
                     TGIQQLAKKYGFIDVDRVGIWGHSGGGFATAGAMFRYPDFFKVGISESGNHENRNYED
                     DWGERYDGLLVKNADGTDNYTAQANQNLASHLKGKLMLAHGLMDNNVPPTNTLLVVEA
                     LEKANKDYDLVIFPNSPHGYGPNGPYMMRRRWDYFVTNLMGGRPPHEYQMKPKTDPRN
                     AME"
     gene            4121..5272
                     /locus_tag="VGQ51_01195"
     CDS             4121..5272
                     /locus_tag="VGQ51_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEV9035196.1"
                     /translation="MKKIYLLLAAVVLISWGVTGHRTVGKIADAHLSPNARAGVRDLL
                     GSETLSDVSTWADEIRGQEKYRQTGPWHYINLPLGLNYDQFKARVENMLESNVYSALG
                     QQMQLITDKSASREQKIEALKFVVHFVGDLHQPMHVSRAEDKGGNTVQLNYEGQGTNL
                     HSLWDSKLIEHTGLDYQQLAEKCDHATPAQVRQWQSDPIVKWMWESYEITSRLYTEVD
                     TMSSRSIGQDYYTAHWPIIQQRLEQAGIRLAGVLNVLFKNGAVTVGASRAAGAGELQS
                     AGASQSAGASQSAAAPTRIDIKDAASHANENVVVSAKVYGYKALEGMTLVNLGAAYPD
                     QLMTVVLRGDAVAIAAGLDGATIRVTGKIELYRGKPEIVVKDPKMITKE"
     gene            5363..6163
                     /locus_tag="VGQ51_01200"
     CDS             5363..6163
                     /locus_tag="VGQ51_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV9035197.1"
                     /translation="MFWNFHKILVICTGGAMIFSPNTTNRTEMEGGGSVVTRSVKHRA
                     EEKPYCIFLSWDDGPSEGSQSVNELALKDSLSINVFLIGRNACLTERSRAMVLEYAAN
                     PMVEIGNHSFTHANRKYEAFFRDPEVVLADFNRSRDSLHLTNGLTRLPGRNFFRLGSF
                     SRNDHNNGKESADVLAANGYSVFGWDLEWRRKAGHGISTHTGEAMFQIVGEMLEKKRT
                     FVPGYLVLLLHDQDCRDGHFRDELEEFIRLAREDGRYRFGHLSEMTGN"
     gene            6208..>6765
                     /locus_tag="VGQ51_01205"
     CDS             6208..>6765
                     /locus_tag="VGQ51_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035198.1"
                     /translation="MVNRRFIILLLCSFPASMVYCQEGGIRVEEIRDTTGGQRGFHQE
                     DIKDWLIQRKVLRDKPPKKNFLLIIPVVASNPTAGFMFGAGMTYAFKVSGTGTKMSTA
                     TANATYSSRGLVNLNMKSNAFFANDRLVLNGDWRYLVNSETTYGLGTTNYISGSIDIN
                     GYDTSTDSIGESLRYHQVRIHETGSW"
BASE COUNT         1537 a         1504 c         2195 g         1529 t
ORIGIN      
        1 ggggggcggt ttgcggcgag atgatggggt tggggggggg cggttggcgg cgagattggt
       61 gcggggtctg gggggcgaat cgcggaattt gtatattcgg gtatggaaaa acgaaatgct
      121 ttccggcagc tggccttgtt gctgttgccg gctttgtcca taggcctgat ggcggacgcc
      181 caatccgccg ggtctgcggg tccgcaggta cacactattc atgggatcat agaaggcgtc
      241 atcgggaaga gcggagtccg cagcttcaag gggatacctt atgccgcgcc gccgataggt
      301 cagctgcgtt ggaaggagcc gcaggatgga gcagattgga cgggggtacg tgtttgcgac
      361 cgttttgggc ccagggcgat gcagccgccg atctattcag atatggtatt ccggtcggcc
      421 ggtatgagtg aggactgttt gtatttgaat gtgtgggcgc cggcgggtgc gtcgccgggc
      481 ggcgggcgta agctgttgcc ggtgctggtc tatttttatg gcggagggtt cgtagcgggg
      541 gatgggtccg agttgcggta tgacggcgaa agcatggcga cgaaggggat agtgagcgtt
      601 acggtcaact atcggctaag tatattcgga ttttttgcgc acccggacct gacgaaagaa
      661 tctccgcatc attcatcggg caattacgct ttgctggatc agacggcagc cctgcgttgg
      721 gtgcagcaaa atatcgccaa cttcgggggc gatccgcgcc gtgtaacgat tgcaggagag
      781 tcggccggat ctatatccgt cagcgcgtta atggcgacac cattaacgaa aggaatgatg
      841 gcgggggcca ttggcgaaag cggcagtctt ttgggcgcgc tgcctccagt cggtgtgagc
      901 caagccgagg agacggcagt tcagtttaca gggacggtcg gggcgacatc gctggctgaa
      961 ttaagggcca tgccggctga ttcactgctg gcggcggcta tgaggtttgg gcctttccgt
     1021 tttcacatga cgatcgacgg ctattttttc ccgaagagtc cttatgatat ctacgcgtcc
     1081 ggtgaacagg cacatatacc gttgctggtg ggatggaatt cgcaggaggg ggattatcat
     1141 gctattttag gtaatgcagc gccgacggtg gctaattatg aagcggcggt ccggaaggcg
     1201 ggtggggttt ttgcggatac gatcctgcga ctgtatcatg cttctgctga tgccgaagtg
     1261 gagggcgttg cgactgcgct ggccagtgac aggtttattg cgtttagcac gtggcggtgg
     1321 gccgatctgc aagggaagac tgggggtcag cctgtttacc gttactttta tgagcgtccg
     1381 cgacctgcta cggtggcagg cgagcctgct gccaaaggag ccgtgcattc ggcggagatc
     1441 gaatatgcga tgggtaatct ttctacgaat aaggtgttcg catggacttc ggccgactat
     1501 gcggtgtcgt cggtgttgca gggatatttc gtcaatttta taaagaaggg tgatccgaat
     1561 ggagcgggtt tgccgcattg gcctgcggcc aatcaggggt ctgttgtgtc ggtgcagcat
     1621 attggggtga agacgaggac ggagccggag cagcatcggg atcggtatga gtgggcgagt
     1681 aagcgataag tcggcggaga gtgtgatgat cggggtgatc gtaatatata cggtgggcca
     1741 catcggtgat cgaatggggc atttgccata tatttagccc ctgagaaaac acaaatggct
     1801 ccgaatatga ataaaatcct tcttaccgtg atctgcgccg cggttaccgt tacggctctc
     1861 gcgcaggaaa cccgagttta tacgaatgcg gattatgagc gtgccgagag catgctaagc
     1921 tataacacag gaccgctgat cgaccgggcg gatgtgcgtc ccacctgggt tgaaggggac
     1981 cggttctggt accgcgtcct gacggctacc ggcagcgagt atgtattggt agaccctttg
     2041 aagaagacgc gtactgctta tccggacaga gaaaagttat ttgctgcgat atctgccctc
     2101 accgggaagc ctgttgaggc tgcgccacgc gaagggggtg gccgtcgcgg attcaggggt
     2161 gaaggcggag gcaactccgt actatcgccg gacggaaaac gcgccgcatt catcagggac
     2221 tataatctct gggtgcggga tgtggcgacg aaacaggaga cgcagctgac cacagacggc
     2281 gtgaaagatt tcggttattc cacggataat gccggctgga agagcagcga caggccggta
     2341 ctgcggtggt cgccggatgg gaagaaaatc gccaccttca aggatgatca acgcaatgta
     2401 ggcgagatgt acctggtctc gaccaaggta ggtcacccag tattgcatgc ctggaaatat
     2461 cctttgccgg gggattcggt aatccccatg atcacacgcg tcatcatcga tgtggatgcg
     2521 ccgaaggtga ttcccctgaa tataccgccc gacccgcatc gtgctacgct cagcgatgat
     2581 atttccagca gcggaacgtt cgacgacgta gattggggca cggacgaaaa tcagttggcg
     2641 tttgtatcta cttcgcggga tcataaggac gagaaattcc gtattgccaa tgcgctgacg
     2701 ggggaggttc gggatgtctt cgaagagaaa gtggctacac agtttgaatc ggggcgcggc
     2761 actatcgatt ggcggtatct gcagggcacg catgagatca tctggtattc cgagcgggac
     2821 aactgggggc atctttatct ctacgatgct tcgaccggac aattgaagaa ccagatcacg
     2881 aagggggatt ggctggtatc gagactggtg aaggtagacg aaaagaaaag agtcatttat
     2941 tttatgggtg cgggcaagga gccggggtgt ccttatttca actatctgta taagataggg
     3001 ttcgacggta agcatatggt cctgttgacg cctgagaaag ggaatcatac gatcacgttc
     3061 tctccgacgg gcgagtattt cgtcgacacg tattcgcagc cggacgtagc gccggtgtcg
     3121 gtattgcgca accaggaagg caagctggtc agcgatctcg aaaaagccga tatttcgcgt
     3181 ttggtcgcat cgggttggag gcccccgata ccgatcacgg ttaaggcgca cgacgggcag
     3241 acggatttgt ttgggttgtt gtttgtgcct tctcatctgg attcgacgaa gaagtatccg
     3301 atcatcgatt acatctaccc cggtccgcaa ggcggcagtg tcggcaactg gggattccag
     3361 gcttcgcgca acgacaacca ggcccttacc gagctgggtt ttatcgtcgt cgagatagaa
     3421 ggtacgagca atccgtatcg ttcgaagtct tttcatgaca tgagctatgg taatatggcg
     3481 gagaatacgt tgcccgacca ggtaacgggt atccagcagt tggcgaaaaa gtatggcttt
     3541 atcgatgttg accgcgtcgg tatctggggt cactccgggg gtgggtttgc gactgcgggt
     3601 gctatgttcc gttatcccga tttcttcaaa gtcggcatat cggagtcagg gaatcatgag
     3661 aaccgcaact atgaggatga ctggggcgag cgttatgacg ggttattggt aaagaatgcc
     3721 gacggaacgg acaattatac ggctcaggcc aaccagaacc tggcgtcgca tctgaagggt
     3781 aagctgatgc ttgcgcatgg tttgatggac aataatgtac cgcctaccaa taccttgctg
     3841 gttgtggagg ccctggagaa ggccaataag gactatgacc tggtgatctt ccccaacagc
     3901 ccgcatgggt atggccccaa tggtccgtat atgatgcgtc gtcgctggga ttatttcgtc
     3961 accaatctta tgggagggcg gccgccgcat gaatatcaga tgaaaccgaa aacggatcca
     4021 aggaatgcga tggaatgacc gcgggtggaa aggccgacgc acatggaatg accgcgggtg
     4081 gggggtccac cgcacatagt tttttgcgta tcttcccgcc atgaaaaaga tatatctctt
     4141 attggccgcc gttgtactta tttcctgggg tgtcaccggt caccggactg tcggcaaaat
     4201 agcggatgcg catctgtcgc cgaatgcacg cgcgggggtg cgcgatctgc ttgggagcga
     4261 gacactatcc gacgtgagca cctgggcgga tgaaatacgc ggccaggaga aataccgaca
     4321 gacgggtcca tggcattata tcaatcttcc gctcgggctt aattacgatc aattcaaggc
     4381 gcgggtcgag aacatgctgg agtccaatgt atacagtgca ttggggcagc agatgcaact
     4441 gatcaccgac aaatcggctt cacgggagca gaagatcgag gctttgaaat tcgtcgttca
     4501 ttttgtaggc gacctgcatc agcctatgca tgtcagccgt gcggaggata aaggcggcaa
     4561 tacggtgcag ctgaactatg aaggacaggg caccaactta cattccttat gggacagcaa
     4621 gctgatcgag catacaggat tggattatca gcagctggcc gaaaaatgcg atcatgccac
     4681 accggcgcag gtcaggcagt ggcagagcga tcctatcgtg aaatggatgt gggaatcata
     4741 tgagattacc tcgcgattat atacggaagt ggataccatg agcagccgta gtatcggcca
     4801 ggactattat accgcgcatt ggcctatcat tcagcagagg ttggagcagg cggggatacg
     4861 gctggcggga gtgttgaatg tgttgttcaa gaatggtgct gttaccgtgg gggcttcacg
     4921 agctgccggt gccggggaat tgcagtccgc aggtgcgtcg cagtccgcag gcgcatcgca
     4981 gtctgcggcg gccccaaccc gcattgacat taaagatgcg gcgagtcatg cgaatgaaaa
     5041 cgtggtcgtt agtgcgaagg tatatggtta taaggcgttg gaaggaatga cgctggtgaa
     5101 tcttggagca gcctatccgg atcagctgat gacagtcgta ctgcggggag atgcggtggc
     5161 catcgccgcg ggactggatg gggctacgat acgggtcacg ggaaagatag agctctatag
     5221 gggcaaacct gagatcgtgg tgaaggatcc gaagatgatc acgaaggagt gagtagattt
     5281 atatcttttg gcaaagtgat gggggccgaa ccgcaggttc ggcccttttt tttgtaaatt
     5341 gacagtccgt caatgaggag tcatgttctg gaattttcac aagatcctgg tcatttgcac
     5401 cggtggcgcg atgatctttt ctcccaacac caccaatcgg acggagatgg agggcggcgg
     5461 cagtgttgtg acccgaagtg tgaagcatcg ggcagaggag aagccttatt gtatcttcct
     5521 aagttgggac gatgggccat cggagggcag tcagagcgtc aacgaattgg cgctgaagga
     5581 cagcttatcc ataaatgtat tcctgatcgg caggaatgcc tgtttgacgg agaggagccg
     5641 tgcgatggtg cttgaatatg cagcgaatcc aatggtggag atcggcaatc acagttttac
     5701 ccacgccaac aggaagtatg aagctttttt cagggacccg gaggtggtgc tcgccgactt
     5761 caaccggagc cgggacagtc tgcatctgac caatgggctg acgcgtctgc ccgggcggaa
     5821 ttttttccgg cttggcagct tcagcaggaa tgatcataac aatggcaagg aatcggcgga
     5881 tgtgttagcc gccaacggat acagtgtttt tgggtgggac ctggaatggc gtcggaaggc
     5941 tgggcatgga atatcgacgc atacgggtga agcgatgttt cagatcgtgg gcgagatgct
     6001 ggagaagaag cggacgttcg tgccggggta tctggtgttg ttgttgcatg accaggattg
     6061 cagggatggg catttccgcg atgagctgga ggaatttatc cggctggcgc gggaggatgg
     6121 gcgttataga tttgggcatt tgagcgagat gacggggaat tgaatggcgg aacggtagtg
     6181 cctgaagtgt cataccagag ccctcgtatg gttaacagac gcttcatcat attgctgctg
     6241 tgcagcttcc cggcctcgat ggtatactgc caggaggggg gaattagggt ggaagaaatc
     6301 agggatacga ctggtgggca gcggggcttt caccaggaag atatcaaaga ttggctgatc
     6361 caaagaaaag tcctcaggga caaacctccg aaaaagaatt tcctgctcat tattcccgtg
     6421 gtcgcgagta atccgacggc gggctttatg ttcggcgctg ggatgaccta tgccttcaaa
     6481 gtgagcggga ccggtacgaa gatgtcgacg gccaccgcca atgccaccta ttcgagcagg
     6541 ggtctcgtga acctgaatat gaagagcaat gcttttttcg cgaatgatcg attggtgctg
     6601 aacggggatt ggcgttacct ggtgaatagc gagactacgt atggtctcgg gacgaccaac
     6661 tatatatccg gaagcatcga tatcaatggt tatgacactt cgaccgacag tatcggggag
     6721 tcgttgagat accaccaggt acggatacac gaaaccggat cctgg
//
LOCUS       DASYSV010000040         6615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2442213, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000040 DASYSV010000000
VERSION     DASYSV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442213"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VGQ51_01210"
     CDS             <1..328
                     /locus_tag="VGQ51_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV9035199.1"
                     /translation="AGFFFQPPQTYDLDAVRPKWTAAKNLFFVELIRAWQLRKANDWE
                     SHSLETGFKEMAAAAAIKPGDLLQPLRIMLVGGRFGPAVFDIAALLGKDETIRRISHV
                     LSLLET"
     gene            379..801
                     /locus_tag="VGQ51_01215"
     CDS             379..801
                     /locus_tag="VGQ51_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEV9035200.1"
                     /translation="MTAEKNTRPIRVLVAKVGLDGHDRGARVIATALRDAGMEVIYTG
                     LRQTPEMVVNAALQEDVDAIGISILSGAHMTVFPRIITLMKEKSMTDVLLTGGGIIPE
                     DHRQQLQNLGVGQLFPPGTPTADIAGYIRAWVRDNREF"
     gene            845..1642
                     /locus_tag="VGQ51_01220"
     CDS             845..1642
                     /locus_tag="VGQ51_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV9035201.1"
                     /translation="MYKTLLTELSDNILTVTIHRPDKLNAINKDVMNELGEVVTEIYT
                     NKDIRSAIITGSGQKAFIAGADISEFQGLTGEQGSALSAKGHAVFNRIASSPKPIVAA
                     VNGFALGGGCELAMACHFRLCTTNAKFGQPEVNLGVIPGYGGTQRLVQLIGKGRALEI
                     MLTGRMIDATEAHTLGLVNYIVTEQNELLTKTREILSIINQKAPIAISRVIECANIAA
                     GDTIAVGTGDNGFLREQEAFGECFATEDMKEGASAFLEKRKPHFRGA"
     gene            complement(1773..2417)
                     /locus_tag="VGQ51_01225"
     CDS             complement(1773..2417)
                     /locus_tag="VGQ51_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035202.1"
                     /translation="MSADEFQQLWKSYEEKLQKSMKLNLALMEANLNRKARVAFNWQI
                     AFKIMMIGLGLAWNIFVGGLMWRYRTEPAFVVAALMVLSCTGYAIGGYFLQVLVMLRV
                     NLSDSIVATQKQLAMLEVLMVRTLRVPFLQTPAYFFFFVTRHMMATSGPWFWLIEGLL
                     VAVSIVVTIWVYRSITLENAARKGWVRRIVDNEGWKAVKRAREYAREIEEFREE"
     gene            complement(2414..2911)
                     /locus_tag="VGQ51_01230"
     CDS             complement(2414..2911)
                     /locus_tag="VGQ51_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9035203.1"
                     /translation="MNHSEFLRLVEDNKGILYKISSSYCRHAGDREDLIQEMIYQLWR
                     SSDKYDNTQKFSTYMYRIALNVAISFHRRTSRGGLKVALDADVELVEEEGMVELDERV
                     EMLQRWIRSLGELDKALMILYLEERPYREIAEILGVTETNVGTKIGRLKERLKKQLIM
                     QNKFL"
     gene            3007..4854
                     /locus_tag="VGQ51_01235"
     CDS             3007..4854
                     /locus_tag="VGQ51_01235"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV9035204.1"
                     /translation="MFRSGTAQLLTILLLLTGRPATIAQTSTSLHYTLTVDSSDLSGY
                     NVDIRIEHPPRRFRLAMATHNEYDDRFWRYVTGFHVTRPAFAVHTDSAVWAVITTGDE
                     VTVSYRISLPPPAPLHFSHRPFLTSYGGLVGDLHSFMYIVDYLDIPCALTLRLPPGWT
                     SADGIYTEEIRVRQTWGKRYYGAVNVSSTSQTQLLDAPILIGRLHRWTFTTDSIRHDV
                     VYLSPSPNLNFDTVSLINNLQKIAHATYSLTGGFPFLYYNFLIEDASAGALEHASSLT
                     IGAKPSDLNNPASGIYEDIAHEFFHAWNGMSIRPSGYTELNYGPQQLTTSLWFTEGVT
                     MLCADLISRRIGLPTEDSTRLSHLTHLITRYYRDTGNSVLPPAKVSLADYAQPGPLGD
                     YSASTHLQGELLGTCLDLLIRDATEGRYSIDALMKEVNYICSAGKQPLTDSVIDSAAA
                     IICQNNAAVHRFFREHLYQGKPIDFATYLHLLGLKIQHDQPTAKDPQGRRLPDIRVYS
                     WILRDDTSLRIGISNPKGCWAKAGLHTGDIIMAINGRPTNSRQDFQSAIRNLHIGDTA
                     RVTVKKQTGTNIVPVYMTGYTTPLITITEDPAATPKQKRLFRAWSSGAP"
     gene            complement(4904..>6615)
                     /locus_tag="VGQ51_01240"
     CDS             complement(4904..>6615)
                     /locus_tag="VGQ51_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV9035205.1"
                     /translation="LLMEQIDKLKKGEFDETLVKAIVANGKLDLLEGLQKNESRVTDL
                     MEGFIQHRDTLWNREVAELDEQAGISKNEIVRVANKYMANNYVLLYKRKGEDKSIMKV
                     DKPPITPVETNAGKQSVFVKKIESMPVSDVTPQWLDFKDGIGHGKAGIADVLYVPNKE
                     NDLFHLYYRLDVGKDNDALLPLAATYLQYLGTDKHPTAEISKAFYDIACNFSINTTSD
                     VTTVTITGLQENFGKAVSLFEDVLHNCLPDTAALVALKDRLTKARANNKLNKNAIMQG
                     LISYARYGSKNPFNTTLSVQEVADLKAEDLTRVLHNLLDYPHTIIYYGPLSMDAFISS
                     IGKLHPLPTAWTAYPAKKHFPFATQSANQVLFANYDMVQSEICWIRNSGSYDVNKEPV
                     VDLFDNYFGGGMGSVVFQTLRESKALAYATYATYGTPMKKEDPFYVLAYIGCQADKMN
                     EAIGGMNALLNDLPENEQNFELARTSEKKDIETQRFTEDGIVFAYLSAKEKGLDYDQR
                     KDEYSAIAKLTMDDVKKFHTQELAGKPYTYCVVASDKKIKMDDLKKVGDVKVLTLDEI
                     FGY"
BASE COUNT         1521 a         2250 c         1396 g         1448 t
ORIGIN      
        1 ggccggattc ttcttccagc cgccgcaaac atatgatctc gacgccgtcc gcccgaaatg
       61 gacagccgca aaaaatctct tcttcgtcga actcatccgc gcatggcagc ttcgcaaagc
      121 caatgattgg gaaagccatt ctctcgagac aggcttcaag gaaatggccg cagccgctgc
      181 aattaaaccc ggcgatctgc tgcagccgct tcgtattatg ttggttgggg gaaggtttgg
      241 acccgctgta ttcgatatcg ccgccttact cggcaaggac gagaccatcc gccgcatcag
      301 ccatgtgctg agcctgctcg agacctgagg cggccccatc gccggcgcta tacaattttg
      361 cctaacttca cattcgatat gacagccgaa aaaaacaccc gccctatccg cgtactcgtc
      421 gccaaagtgg gcctcgacgg acacgatcgc ggggcccgcg tgatcgccac cgccctccgg
      481 gatgccggta tggaggtcat ctatacgggt ctgcgtcaaa cacccgaaat ggtggtcaat
      541 gccgcattac aggaagacgt cgacgccatc ggcatcagca tcctcagcgg cgcccatatg
      601 accgtcttcc cccgtatcat cacactgatg aaggaaaaaa gcatgacgga tgtcctgctc
      661 accggcggcg gcatcattcc cgaggaccat cggcagcagc tacaaaacct cggagtaggc
      721 cagctcttcc cgcccggtac gcccacagcc gatatcgccg gctatatccg cgcctgggtc
      781 cgcgataacc gcgaattcta aacatcgcaa ttcatccacc cccaccgcca aaaactcttc
      841 tcccatgtat aagacattac tcaccgaact ctccgacaat atcctcacag tcactatcca
      901 ccggcccgac aaactcaacg ccatcaacaa agacgtcatg aacgagctcg gcgaggtcgt
      961 gaccgagatc tataccaaca aggacatacg tagcgccatc attaccggct cgggccagaa
     1021 agccttcatc gccggcgccg atatctccga attccaggga ctcacgggcg aacagggctc
     1081 cgccctctct gcaaaaggac acgcagtctt caatcgtata gcctcctccc cgaaaccgat
     1141 agttgccgcc gtcaatggat tcgcgctcgg cggaggctgc gagctggcca tggcctgcca
     1201 tttccggctg tgtacgacca atgcaaaatt tggacagccc gaggtcaacc ttggcgtcat
     1261 tcccggatat ggcggcacgc aaagactggt gcaactcatc ggaaaaggcc gtgctctcga
     1321 gatcatgctc accggccgta tgatcgacgc caccgaagcg cacaccctgg gcctcgtaaa
     1381 ctatatcgtc accgaacaga acgagctcct caccaaaacc cgcgagatcc tctccatcat
     1441 taatcaaaaa gccccgatcg ccatctcccg tgtcatcgaa tgcgccaata tcgcggcggg
     1501 agacaccata gccgtcggca ccggcgacaa cggcttcctc cgcgaacagg aggccttcgg
     1561 cgaatgcttc gccaccgaag acatgaaaga aggggcctcc gcctttctcg aaaagcgaaa
     1621 gccccatttc cgcggcgcct gacggcgccg catccacccg ccgcgtccat ccgccgcatc
     1681 catccgccgc acccaaccac ggcatccacc cgccgcgtcc atccgccgca cccaaccacg
     1741 gcatccaccc gccgcatccc gccgctcaca cctcattcct ccctgaattc ctcaatctct
     1801 ctcgcatact ccctcgcgcg cttcaccgcc ttccaaccct cattatccac tatcctcctc
     1861 acccatccct tccgcgcagc attctccagc gtaatggacc gatagaccca tatcgtcacc
     1921 acgatcgaca ccgccaccag caacccctcg atcaaccaaa accacggccc gctcgtcgcc
     1981 atcatatgcc gcgtgacaaa gaagaaaaaa taagccggag tctgcaggaa cggcaccctc
     2041 agcgtacgta ccatcaaaac ctcgagcatc gccagctgtt tctgcgtagc cacaatgcta
     2101 tcgctcaaat tcacccgaag catcaccagc acctgcaaaa aatacccccc gatcgcatac
     2161 cccgtgcacg acagcaccat caatgccgcc accacgaacg ccggctctgt acggtatctc
     2221 cacatcaatc ccccgacaaa aatattccac gcgagaccca acccgatcat catgatctta
     2281 aaggcgatct gccagttgaa cgctaccctg gccttccggt tcagattggc ttccatcaaa
     2341 gccaaattca atttcatcga tttttgcagc ttctcctcat aagacttcca cagctgctga
     2401 aactcatctg cactcataaa aatttgtttt gcatgatcaa ttgttttttc aaccgctcct
     2461 tcaacctccc tattttcgta cccacattcg tctccgttac ccccagtatc tccgcaatct
     2521 cccgataagg acgctcctcc aggtaaagga tcatcaatgc cttgtccagc tcgcccagcg
     2581 atcggatcca tcgctgcaac atctcgaccc gctcgtccag ctcaaccatc ccttcttcct
     2641 ccaccagctc cacatccgca tccaacgcga ccttcaatcc accgcggctg gtccgtctat
     2701 ggaacgatat agccacattc aaagcgatcc ggtacatata agttgaaaat ttttgggtgt
     2761 tgtcgtattt atccgaactc cgccacaact ggtagatcat ctcctggata aggtcttccc
     2821 ggtcgcctgc atgccggcag tacgaggagc ttatcttgta aaggatccct ttgttgtcct
     2881 ccaccagtcg aaggaattcg gaatggttca tactatatta ttcgccggaa ggtccaaaaa
     2941 atcacatata tttaaataaa tttcgcaatt cccaacatcg caactcctaa cttctccttt
     3001 cctgccatgt tccgttccgg caccgcgcaa ttgctaacca tcctcctcct gctcaccggt
     3061 aggcccgcaa ctattgccca aacctcaacc tccctccatt ataccctgac cgtcgactca
     3121 tctgacctat ccggctataa tgtcgacatc cggatcgaac accctccccg ccggtttcgc
     3181 ctggccatgg ccacgcataa tgaatacgat gaccgcttct ggcgctacgt caccggcttc
     3241 catgttacga gacccgcatt tgccgtacac acggacagcg ccgtatgggc cgtcataacc
     3301 accggcgacg aagtaacggt atcctatcgt atcagcctgc cgccacccgc accgcttcat
     3361 ttttctcacc gccccttcct gacctcctac ggcggcctcg tcggcgatct ccattcattc
     3421 atgtatatcg tggattacct ggatatcccc tgtgcgctca cactacgtct tccgccagga
     3481 tggacttcgg ccgacggaat atacacagag gagatcaggg tccgacaaac ctggggcaag
     3541 cgctactacg gtgcagtgaa tgtctcttcc acttcccaga cccaattact cgatgccccc
     3601 attcttatag gccgattgca tcgttggacc ttcaccaccg acagcatccg gcacgatgtc
     3661 gtctatcttt cccccagccc caacctcaac ttcgacaccg tctcattgat aaacaatctc
     3721 caaaagatcg ctcatgcaac ctacagcctc accggcggct ttccgttcct atattataac
     3781 ttcctgatcg aagacgccag cgcgggcgcg ctcgaacacg cttcctcgct taccatcggt
     3841 gcgaaaccgt ccgatctcaa taaccccgcc tcaggcatct atgaagacat cgcgcatgaa
     3901 tttttccatg cctggaacgg gatgtccatc cggccttcgg gctataccga actcaactat
     3961 ggtccgcaac aactgacgac cagcctctgg ttcaccgaag gtgtcaccat gctctgtgcc
     4021 gatctcatct cgcgccgcat cggcctgccc acagaagact ctacccgtct ttcccatctg
     4081 acccatctca tcacacgata ttatcgagac accggcaaca gtgtcctccc cccggcaaaa
     4141 gtcagtctcg ccgactacgc ccagcccgga cccctcggtg attattccgc cagcacccat
     4201 ctccagggcg agctgctcgg cacctgtctc gatctcctta tccgtgacgc caccgaagga
     4261 cggtactcca tcgacgcgct catgaaggaa gtcaactaca tctgcagcgc cggcaagcaa
     4321 cccctcaccg acagtgtgat tgatagcgcc gctgccatca tttgtcaaaa caacgccgcc
     4381 gtgcaccgct tcttccgcga acacctctat cagggcaaac ccatcgattt cgccacttat
     4441 ctccacctcc tcggtctcaa aattcaacac gaccagccca ccgcaaaaga cccgcaaggc
     4501 cgccgccttc ccgacatccg cgtctattcc tggatcctcc gcgatgacac cagtctccgc
     4561 atcggcatct cgaatcccaa aggctgctgg gccaaagcgg gcctccacac cggcgacatc
     4621 atcatggcca tcaacggcag accgaccaac tcccgccagg acttccagtc cgccatccgc
     4681 aaccttcaca tcggcgacac cgcccgcgtc accgtaaaaa aacaaaccgg gacgaatatc
     4741 gtcccggtct atatgacggg ttatacaacc cctttaatta ccatcactga agaccccgcc
     4801 gccactccca aacaaaaacg cctcttccgc gcatggtcct ccggcgcacc ctgaagcccg
     4861 cgccactcca ccacccagaa cttcgcgccg cgccgcagcc tccctagtaa ccaaatatct
     4921 catccagcgt caacaccttc acatccccca ccttcttcag atcatccatc ttgatcttct
     4981 tatcactggc cacgacgcag taagtatagg gcttccccgc cagctcctgc gtatggaatt
     5041 tcttcacatc atccatcgtc agctttgcaa tggccgaata ttcatccttt cgctgatcat
     5101 aatccaaccc tttctccttc gcactcagat aagcaaacac gatcccgtcc tcggtgaacc
     5161 gctgcgtctc gatgtccttc ttttcactcg tccgcgccag ttcgaagttc tgctcgtttt
     5221 ccggcagatc gttcagcaac gcgttcatac cgccgatcgc ctcattcatc ttgtccgcct
     5281 ggcaacctat ataagccaat acataaaagg gatcttcttt cttcatgggc gttccatagg
     5341 tagcatacgt cgcatacgcc agtgccttcg attcgcgcag cgtttgaaaa acgactgaac
     5401 ccataccccc tccgaaatag ttatcgaaaa gatccactac cggctcctta ttgacatcat
     5461 aggaacccga attgcggatc cagcagatct cgctttgcac catatcgtaa ttggcgaaga
     5521 gcacctggtt ggccgattgc gtcgcgaaag gaaaatgttt cttcgccgga taagccgtcc
     5581 aggccgtcgg caggggatgc aatttcccga tggatgatat aaaggcatcc atcgacagcg
     5641 gtccatagta gatgatggta tgcggatagt ccagcaggtt atggagcacc cgcgtaagat
     5701 cctcagcctt cagatcggcc acttcctgga cggaaagcgt cgtattgaag ggattcttgc
     5761 tcccataacg tgcataggag atcaaaccct gcatgatcgc attcttgtta agtttgttat
     5821 tggcacgcgc cttcgtcagc cggtccttca acgctaccag cgcggcggta tcgggaagac
     5881 agttatgcaa tacatcctcg aaaagtgaca cggccttacc gaaattctcc tgcagaccgg
     5941 tgatggtgac cgtcgtcaca tcgctggtgg tattgatgct gaagttacag gcgatgtcat
     6001 agaaggcctt gctgatctcg gccgtgggat gtttgtccgt tcccaggtat tgcagatagg
     6061 tcgccgccaa cggtaacagc gcgtcattat ccttacccac atccagacga tagtacaggt
     6121 ggaacagatc gttttcctta ttggggacat acaatacatc ggctatcccg gccttgccat
     6181 gtccgatgcc gtctttgaaa tccagccact gcggggtcac atccgatacg ggcatgcttt
     6241 ctattttctt gacgaatacg gactgttttc cggcattcgt ctccaccggc gtgatcggcg
     6301 gtttgtccac tttcatgatg ctcttgtctt cacccttccg cttgtacagc aacacataat
     6361 tgttggccat atacttattc gcgacacgca cgatctcgtt tttggagata ccggcctgct
     6421 cgtcaagctc ggcgacttcg cggttccata acgtgtcccg gtgctggata aagccctcca
     6481 tcagatcggt cacccgcgat tcgtttttct gaagaccttc gagcagatcc agtttcccgt
     6541 tggcgacgat agcctttacc agggtttcgt caaactcgcc cttcttcagc ttgtcgatct
     6601 gctccatcag caggt
//
LOCUS       DASYSV010000041         5490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2187611, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000041 DASYSV010000000
VERSION     DASYSV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187611"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VGQ51_01245"
     CDS             <1..562
                     /locus_tag="VGQ51_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEV9035206.1"
                     /translation="DRDTSGVIVFAKDESTHKYLSGIFEDRKVEKIYQGIVQGTLPQP
                     QGSIDQPIMEHPAKPGVMVINRRGKASLTDYQVLEDLNLFSLVQFRIHTGRTHQIRVH
                     MQALGHPIVADDLYGDGRPVFLSAIKRSYKLSKSEEQERPILSRLALHSHTLAFTDAA
                     GTPHKFEAPLPKDMRALLQQLRKSKK"
     gene            complement(576..1223)
                     /locus_tag="VGQ51_01250"
     CDS             complement(576..1223)
                     /locus_tag="VGQ51_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEV9035207.1"
                     /translation="MEKRSFSLHVSPGVGKVTGEYIVAPDCTAIMTLAHGAGADMQHP
                     FMVELATALAGAGIGTLRFNFPFMEGRRGRPDKPAVAHETVAAAITHAAATYPALPLY
                     VAGKSFGGRMSSQYLAACPRAEVKGVVFYGFPLHPPGKESVERADHLREVTVPKLFLQ
                     GTRDELAEWDLVYAVCSSLRKTRLVKIEGANHAFKAGKRDVIAMLVKATAEWIGK"
     gene            1289..2005
                     /locus_tag="VGQ51_01255"
     CDS             1289..2005
                     /locus_tag="VGQ51_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035208.1"
                     /translation="MATFTGRRRPFPALVIVSLLFLFCLYPGQAWMAHFFPRLAGLAT
                     IDPGLIILGTSFRIPFSIDLNLLPILFLFLYAIIVIIYSISRHQPIGRNVLQRCAAVI
                     SGIFFLVVCTAIGGLISYLLIDLLPARAQNSIAAVGISADIHLPFLGYTTNSLHGNIL
                     SLIGFLAGLVLFIVKVTHNPSSRRPTPLTREQRMTPYKKMLQERRRPAPPLSSNEYLH
                     CRNQPLQTLTPEAVNYRPLG"
     gene            2123..4402
                     /locus_tag="VGQ51_01260"
     CDS             2123..4402
                     /locus_tag="VGQ51_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEV9035209.1"
                     /translation="MRLYRLLLLFISIAPIAADAQIVWNKDGNSYTTIEDNAIVGITL
                     PAMTKTTLISAEQLAPLGLPTRDRSDASRFRGGRGGGAFSYQFSDDHQKVLLYTKPVR
                     IYHDAFYSCWVFDRATGSLTKVAQDRPATSVLTYHLPAGYILNAKLSPDGTKLAYVYD
                     NNIYTEDLATHASSPLTTDGNDRLLNGWFDYAYSEELYTIDGFRWSPDSKSIAYWQNN
                     LAHVGIFYLIDNTDSIYSRPIPIPFSKVGTPIAEARIGVVTLATKATKWMNIEGDPAS
                     HYLPRMEWTPDGAAIIIQQLNRAQDSSKIILASPTTGESKTIYNETDPAWIDIKAFWA
                     HSGGIGWDWIDDGKAFIWASEKDGWRHLYRITLDGKETLLTNGAYDVTSINGWDEKNN
                     ILYFGASPDNATQRYLYRTTLNGATSHARGKSAAATKSAAAASTTVRVTEDLPGTHSY
                     VFSPNGRWAEHNFSSHLYLPATEWLEMPAAKPLDPKDAISAHLRPTPEGKQTTFFRLT
                     TADGVTMDGWVIKPKDFDSTKKYPVIFYTYSEPAGASVSDVANAGGRSSFFNVDSGYI
                     FVSVEGRGTPVPKGRAWRKAAYKSIGWTNVNDQAAAAREVLKWPFTDPTRVAVFGSSG
                     GGSTTQNLLFRYPEIYKVGLASAGVPNQLIYNTIYEERFMGVLPENRDAYIKCNPLTY
                     AKNLQGHLLIMHGTGDHNVNYQGEEMLLNELIKYNRQFTFMPYPNRTHGITEGEGTIL
                     HKTTLMANFLHTWCPPGGR"
     gene            4424..>5490
                     /locus_tag="VGQ51_01265"
     CDS             4424..>5490
                     /locus_tag="VGQ51_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035210.1"
                     /translation="MLTALLAFSGAILLAWLLLPGFNHLSAKDIRFPWANTTFWLAAL
                     AFTIFTGFLAGAYPAFYLSAFNPVRRFTRRTGLFRKVLVVTQFTVSLSLMIATIIIFR
                     QIDFSKDRPTGYDREGLLTVSINTDTLYRHADAVRNELLRTGFISQIARSSWSTDGFW
                     SNSYLTWSGMPPEKQGGLYRDIKVTPEFGATVGWTIVEGRDFSRDFATDSSAIILNEA
                     ALRAAGFKHPIGRMVGFRKKNYHIIGVASNMLTNSPYDNIEPCVFMESKDDLGFFTLR
                     VKRGTAMHGALAAIGTIFNRYNPESPLIYSFNDDAYAQKFAAETRQGNIAIALTILAL
                     FISCLGLFSLASFMAEQRTKE"
BASE COUNT         1247 a         1913 c         1201 g         1129 t
ORIGIN      
        1 cgaccgcgac accagcggcg tcatcgtctt cgcaaaagac gaatccaccc ataaatacct
       61 ttccggcatc ttcgaagacc gcaaagtaga aaagatctac cagggcatag tccagggcac
      121 cctcccgcag ccgcagggca gcatcgacca gcccatcatg gaacatcccg ccaaacccgg
      181 cgtcatggtc atcaatcgcc gtggcaaggc atccctcacc gattatcagg tcctcgaaga
      241 cctaaatctg ttttccctcg ttcaattccg catccatacc ggccgcaccc accagatccg
      301 cgtacatatg caggccctcg gtcatcccat cgtcgccgat gatctctatg gcgacggccg
      361 ccccgtattc ctgtcagcga tcaaacgcag ctacaaactc tccaaatccg aagagcagga
      421 acgccccatc ctctcccgtc tcgcccttca ctcccacacc ctcgccttca ccgacgccgc
      481 cggcacgccg cacaaattcg aagcgccgct gcccaaagac atgcgcgcac tactccaaca
      541 gctgcgaaaa tcaaaaaagt agatcttatt tctctttatt tcccgatcca ctccgccgtc
      601 gccttcacca acatcgcgat cacatccctc ttccccgcct tgaacgcatg attggccccc
      661 tctatcttca ccagtctcgt cttcctcagc gatgaacaaa ctgcatagac caggtcccat
      721 tccgccaact cgtcccttgt cccctgaagg aagagcttcg gcaccgtcac ctcccgcaaa
      781 tgatccgccc gttccaccga ttccttcccc ggcggatgca atgggaatcc ataaaagacc
      841 acacccttca cctccgccct cggacacgcc gccaggtatt gcgaggacat ccgacccccg
      901 aacgatttcc cagcaacata caagggcaga gcaggatagg tggccgccgc atgcgtaatt
      961 gccgccgcaa ccgtttcatg cgcaaccgca ggcttatcgg gccgacccct cctcccttcc
     1021 ataaacggaa agttaaaacg cagcgtcccg atccccgcac ccgcgagcgc ggtcgccagc
     1081 tccaccataa aaggatgctg catatccgcg cccgccccgt gagccagcgt cattatggcc
     1141 gtgcagtccg gcgcaacgat atattccccg gtcactttcc ccaccccggg cgaaacatga
     1201 aggctaaaag atctcttttc cactggtata gtatttgatt ggaatatgtt tataggagga
     1261 ttatgcccaa ttatacaaaa tttttcttat ggctacattt accgggaggc ggcgaccgtt
     1321 ccccgcattg gtcatcgttt cccttctatt cctgttttgt ctttatcccg gtcaggcctg
     1381 gatggcgcac ttctttcccc gcctggcagg actggccacc atcgatcccg gcctgatcat
     1441 cctcgggacc tcgtttcgca tccccttttc catcgatctc aacctcttgc cgatactctt
     1501 cctgttccta tatgcgatca tcgtcataat ttatagtatc agccggcatc aacccatcgg
     1561 ccgcaatgtc ctgcaacgct gcgccgcggt aatttccggc atttttttcc tggtggtctg
     1621 caccgccatc ggggggttga tctcttatct cctcatagac ctgctgccgg cccgcgccca
     1681 aaacagtata gcagccgtcg gtatcagcgc cgacatccat cttccctttc tcggatatac
     1741 caccaattca ctacacggaa atatcctttc ccttatcggc ttcctggccg gtctggtgct
     1801 cttcattgtt aaggtaaccc acaatccttc atctcgccga cccacaccgc tcacgcgcga
     1861 gcagcggatg acaccctaca aaaaaatgct tcaggaaaga cgccggcccg cacctcctct
     1921 ttcctccaat gaatatttgc attgccgcaa ccaacccctc caaaccctca ctcccgaggc
     1981 cgtcaactat cgcccactcg gatgatccgc cgtccgggac gcgcgcgccc ccatacaaat
     2041 tgttaaaatt cgaccagctt gcgccaaaaa caagcaattc cgcccgcccg ttcgacccta
     2101 catttaacca aacattctgc atatgcgcct ctatcgattg ctgctgctct tcattagcat
     2161 cgcccccata gccgccgacg cccaaatagt ctggaacaaa gacggcaact cctacactac
     2221 catcgaagac aacgccatcg tcgggatcac gctccccgca atgaccaaaa ccacgctcat
     2281 cagcgccgaa cagctcgcac ctctcggact acccacccgg gaccgcagcg acgcaagccg
     2341 tttccgcggc ggccgcggag gcggagcttt cagctatcaa ttcagtgacg accatcaaaa
     2401 ggtcctcctc tatacaaaac ccgtccgtat ctatcatgac gcgttctatt cctgctgggt
     2461 attcgaccgc gcaacaggca gtctgacaaa agtcgcgcag gaccgccccg ccaccagcgt
     2521 cctcacctat cacctgcccg ccggctatat cctcaacgcc aagctctccc ccgacgggac
     2581 caaactggca tatgtatacg ataacaacat ctacaccgag gaccttgcaa cccacgcatc
     2641 gtctccgctc actaccgacg gcaatgaccg gcttctcaac ggctggttcg actatgccta
     2701 cagcgaggag ctctatacca tcgacggctt ccgctggagt cccgacagca agagtatcgc
     2761 gtactggcag aacaatctcg cgcatgtagg gatcttctat ttgatcgaca ataccgacag
     2821 catctactcg cgccccatcc ctataccttt ttcgaaagtc ggcacgccca tcgccgaagc
     2881 gcgtatcggc gtcgtcacgc ttgccactaa agccacaaaa tggatgaaca tcgaaggcga
     2941 ccccgccagc cattatctcc cgcgaatgga gtggaccccc gacggcgccg ccatcatcat
     3001 ccagcagcta aatcgtgccc aggactcctc taagatcatc ctcgcctccc ccaccaccgg
     3061 cgaaagcaag accatttaca acgaaaccga ccctgcatgg atcgacatta aggccttctg
     3121 ggcccacagc ggcggcatcg gctgggactg gatcgacgac ggcaaggcct tcatctgggc
     3181 cagcgagaag gatggctggc gtcatctgta ccggatcaca ctcgatggta aggagaccct
     3241 gctcaccaat ggagcgtatg atgtgacgag catcaacggc tgggatgaga agaataacat
     3301 cttgtatttc ggcgcctccc ccgacaatgc cacccagcgc tatctgtatc gcaccactct
     3361 caacggcgcc acatcccatg cccgcggaaa atccgccgct gcaaccaaat ccgccgctgc
     3421 agcctccacg actgtgcgcg tcaccgaaga cctccccggc acacactcct acgtcttttc
     3481 tcccaatggc cgctgggcgg aacacaactt ctccagccat ctctacctcc ccgcgaccga
     3541 gtggctcgaa atgcccgcag ccaaaccgct cgatcccaaa gacgcgatct ccgcgcatct
     3601 ccgccccaca cccgaaggca aacaaaccac cttcttcagg ctcacgacag ccgacggcgt
     3661 caccatggac ggttgggtga tcaagccgaa ggatttcgac tcgacaaaaa aatatcccgt
     3721 tatattctat acgtattccg aacccgccgg cgcctccgtc agcgatgtcg ccaatgccgg
     3781 cggccgctcc agttttttca atgtggacag cggctacatc tttgtgtcgg tagaaggccg
     3841 cggaacaccg gtccccaaag gtcgcgcctg gcgcaaggcg gcatacaagt ccatcggctg
     3901 gacgaatgtc aacgaccagg cggcagccgc gcgcgaagtc ctcaaatggc cctttaccga
     3961 tccgacgcgc gtagcggttt tcggcagcag cggcggcggt tccacgaccc aaaatcttct
     4021 cttccgctat cccgagatct ataaggtcgg tctggccagc gccggcgtac ccaaccagct
     4081 tatctacaac acgatctatg aggagcgttt catgggcgtc ctgccggaga accgcgatgc
     4141 ctatatcaaa tgtaaccccc tcacctatgc gaaaaacctc cagggccatc tcctgatcat
     4201 gcacggcacc ggcgatcata acgtcaacta tcagggcgaa gagatgctcc tcaacgagct
     4261 catcaaatac aaccgccagt tcactttcat gccctacccc aaccgcaccc acggcatcac
     4321 cgaaggcgaa ggcaccatcc tccacaaaac aaccctcatg gccaatttcc tgcatacctg
     4381 gtgccccccc ggcggaagat aatcctccgg tctcggcgag tccatgctca cggccctgct
     4441 ggccttttcc ggcgccatcc tcctcgcctg gctccttctt cccggattca accacctttc
     4501 ggcaaaggac atacggttcc cctgggccaa caccaccttc tggcttgccg cattggcctt
     4561 caccattttc acgggattcc tggccggcgc ctacccggcc ttctatctct cggctttcaa
     4621 tccggtgcgc cgcttcacac gccggacagg actgttccgc aaggtcctcg tcgtgaccca
     4681 gttcacggtc tctctgtccc tcatgatcgc taccatcatc atcttccgcc agatcgattt
     4741 ttcaaaagac agacctaccg gatacgatcg cgaaggtctg ctcacggtca gcatcaacac
     4801 cgacacgctt taccggcatg ccgatgccgt tcggaacgag ttgctccgca cgggattcat
     4861 cagccagata gcccggtcct cctggagcac ggatggtttc tggtccaaca gctatttgac
     4921 ctggagtgga atgccgccag aaaaacaagg cggtctttat cgcgatataa aagtgacccc
     4981 ggagttcggt gccaccgtgg gttggaccat cgtcgaaggc cgcgatttct cccgcgactt
     5041 cgctaccgat agcagcgcca taatactaaa tgaagcagca ctaagagccg ccggctttaa
     5101 acaccccatc ggtcggatgg tcgggtttcg caagaagaac tatcacatca tcggagtcgc
     5161 ctccaatatg ctcaccaact caccctatga caatattgag ccttgcgtat tcatggaaag
     5221 caaagacgat ctcggttttt tcacccttcg cgtcaaacgc ggaacagcca tgcacggcgc
     5281 actcgccgcg atcgggacca tcttcaaccg atacaaccct gagagcccac ttatttacag
     5341 cttcaatgac gacgcctatg cgcaaaaatt tgccgccgag acccgacagg ggaatattgc
     5401 tatcgccctt actattctgg ccctttttat ctcctgtctc gggcttttta gtctggcttc
     5461 cttcatggcg gaacaacgga caaaagagat
//
LOCUS       DASYSV010000042         8697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1291631, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000042 DASYSV010000000
VERSION     DASYSV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291631"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VGQ51_01270"
     CDS             complement(<1..556)
                     /locus_tag="VGQ51_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV9035211.1"
                     /translation="MKAMCIILVMLCGWGLVAAQPVGMMRIPGGNFEPFILTGSQRQM
                     VKVRSFYLDEHAVTNVEFLQFVKANPGWRRSKVVRIFADSSYLKQWAGDLEIGDPRLS
                     KSPVTNISWFAATAYCRWKGKRLPTMAEWEYAAGAAPVRRKGTRDLTRLILEWYDHPT
                     PEVLPAVGSTYKNAFGLYDMHGLVW"
     gene            complement(566..2038)
                     /gene="nirK"
                     /locus_tag="VGQ51_01275"
     CDS             complement(566..2038)
                     /gene="nirK"
                     /locus_tag="VGQ51_01275"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEV9035212.1"
                     /translation="MKKSFVLQKFGLGAARLGPAGLALILLCTISCKHSAEPANPAND
                     TAAVIGEYTAELAHAPSVPLAPTYAHPEKVIVRLEVVEKVMQLADGVKYTYWTFGGSV
                     PGPMIRVQEGDEVEMHLSNNPNNKMPHNIDLHAVSGPGGGAEATLTVPGHTSVFSFRA
                     LKSGLYVYHCATAPVGMHIANGMYGMILVEPKGGFPKVDKEFYLMQGEFYTKGKNGEG
                     GLQPFDMDKAIKEEPEYVVFNGSVGGATGGNALRAKVGDRIRIFVGNGGPNLVSSFHI
                     IGQIFDNVYEEGGSQVHQNVQTTLIPAGGAAIVEFTCREPGVYNIVDHSIFRAFNKGA
                     VAQLVVTGAADKNIYSGKIKDELYQPGAAVAEESDVPAGPPVQDLPLEVRFKNGHELF
                     EMNCSSCHQSTGQGVPGAFPPLSKSDFLMGRPDKGIGVLLNGINNEPITVNGKHFQGV
                     MPKLNLSDDQIADILSYVRNSWGNKGGVVQEKEVKKVRNQ"
     gene            complement(2195..4525)
                     /locus_tag="VGQ51_01280"
     CDS             complement(2195..4525)
                     /locus_tag="VGQ51_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9035213.1"
                     /translation="MKRTAVKCCLWIALCFLLVDGVRGQVVHQGRVIDKDTREPLEGA
                     VVSIRAVSDSAMGRPVHSVLTDKQGMFRMTIRDRGDSLNISFVGYRSYCVCAAACCAD
                     GGCCVDGGCFPEGVCKRAGEYPMIRQAPDLTTITINPAPNASFHILEKVDLGLRPVSS
                     AQDLLRLVPGLFIGQHQGGGLAEHIFYRGFDADHGTDVNLSVDGMPLNLVSHIHGQGF
                     ADMHFLIPELVSRFDYGKGPYYAEYGDFATAGYVAFRTADVLDKNEVKLEGGAFHTGR
                     VLAMLRLPDGRTMSHDQCAYLAGEAAYTDGPFDSPQRLRRLSLFGKYNVNLSAATRLT
                     ATFSTYEGAWRSSGEIPERAVKDGLIGRFGYVDSAQGGNTGRTNLIVRLKTKMGVGWL
                     MEHQVYLSHYYFSLHYDPTFFADDSVNGDQLRQQEQRDLAGYNGRLSHHAYFSDGGEL
                     LSAAGIGSQANFIRLSRLSHTRNENDVMEDLQSGVPREYAASGWLDENYHWGNWMFNV
                     GIRADWLYFRYRDDLASWQAPREKWVVSPKFNVSYTVSPLLQFYFRAGKGFHSNDARV
                     VIANRGLDVLPAAYGADLGVYWKPLPRLLLNAAVWTLSLQQEFTYDADEGTMDPGDRT
                     LRKGIDLSARYQLASWLFAHADVNYCKARDLQAAKSANYLPLAVPLCGTGGLDFRVRN
                     GLNGGLSYRYMKDRPANVDNTLVAKGYCVSDLMVNYTCSRWETGLEIQNLLKTKWREA
                     QFETFSRMKGEPAAVDDISFTPGSPFFIRLKMSVFF"
     gene            complement(4522..5256)
                     /gene="ric"
                     /locus_tag="VGQ51_01285"
     CDS             complement(4522..5256)
                     /gene="ric"
                     /locus_tag="VGQ51_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HEV9035214.1"
                     /translation="MNTNVPNLTPEQTIGELAGKDYRKAEVFRKFGIDFCCGGGQTIG
                     QACHSAGISEGELRSSLDLAELRVRTPEEDFQQWPTGKLAEYIVRRHHEYVRETAPVL
                     TEYARKVAAHHGAAHSELIGLELRVRQTVLDLLGHMDREEKVLFPAVQELAETAEQAG
                     AGNLSFVRHAILQMQNEHSISGEDLEGFRVMTHGYSVPEDACNSYRFLYEKLREFDLD
                     LQQHIHLENNILFPKALALLDKTASE"
     gene            complement(5268..6035)
                     /locus_tag="VGQ51_01290"
     CDS             complement(5268..6035)
                     /locus_tag="VGQ51_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV9035215.1"
                     /translation="MRKIVKYVLLDILRNRMVMSYAVVLLGISLAVLNLEDNSAKALL
                     SLLNIVLIVVPLMSVIFSTVYYYNSVEFIELLVAQPLRRVRILLSLYAGLAFSLLIAF
                     GVGVGIPVLFYLHDVTGVLLVVSGSALTLIFSAVGVLGSVLMRDKARGIGFAILLWFY
                     FSVLYDALVLFLLFQFSDYPLEKPAIVLGCLNPIDLSRILLLLQLDNSAMMGYTGAVF
                     RSFFGSVGGALLALVILLLWMIVPLMAAMRRFNHKDL"
     gene            complement(6049..6840)
                     /locus_tag="VGQ51_01295"
     CDS             complement(6049..6840)
                     /locus_tag="VGQ51_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9035216.1"
                     /translation="MIEISHICKKFGRLSVLDDVSVTLSQGQSIALIGPNGSGKTTLI
                     RSLLGMVKPDKGSIRFNGQDILQGWAYRADMGYMPQIGRYPDNMTIGQVFKMMKEIRM
                     AKEERWVVEANRDRGAGSCAKEGRSLDEELFHAFGLDGMTGKRMRTLSGGTRQKVSAS
                     LAFLFNPSVLILDEPTAGLDPVATGILKEKIQAVREEGRLILITSHILADLDDMVSEV
                     VYLQEGRVKFHKTLSRLRADTGEEKLVRAIAQYMQITGSQQKSHL"
     gene            complement(6837..8156)
                     /gene="nosD"
                     /locus_tag="VGQ51_01300"
     CDS             complement(6837..8156)
                     /gene="nosD"
                     /locus_tag="VGQ51_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEV9035217.1"
                     /translation="MERVVSIILLGLVWPSCGLAKTWEVRKTGGLNSIGAAVRSASPG
                     DTIKVFAGVYLEKGLVIDKRLCLIGIGMPVLDGEGRYEIVLVKADGVTFQGFVLRHSG
                     YSGWNDIAGIHLAGVRGVKVLSNRLDDTFFGLYCQNSDSCVLSGNILVSRAAMGGQTS
                     GAAVGGQTLGAAVGGQTSGAAMSGQAGNGIHCWHCSHMRILDNTISRHRDGIYFEFVT
                     ESLIRGNNSAYNVRYGLHFMFSHNDAYEGNTFRHNEAGVSVMFSHGVDMRGNVFADNL
                     GGGAYGILLKEISDGRIESNRFLHNTIAIYMEGTTRVQINRNLFSGNGWAIKVQASCS
                     EDTISANNFVGNTFDIATNGTLVLNFYDRNYWDKYEGYDIDRDGIGDIPYHPVSLYAM
                     IAEKNPAVMMLYHSLMVSLLDRTEKVVPSITPVNLVDRRPLMKPLAL"
     gene            complement(8135..>8697)
                     /locus_tag="VGQ51_01305"
     CDS             complement(8135..>8697)
                     /locus_tag="VGQ51_01305"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEV9035218.1"
                     /translation="QLLNFGAYSIPDLGGWIFVAVGVVLVGVAVLEWRKGHKVRRLYS
                     GAGAPVLVALAVVVALSSCGSGLQAIRYGQDNCDFCKMAFTDRHFGGEVISKKGKVYK
                     FDDVHCLVTSLKTGVPAAGDVAGIYLVDYNGGPWIKAADAFLLYSENFHTPMNGNIAA
                     FADAAARDAQIIHSEGKAFTWKELYP"
BASE COUNT         2080 a         2782 c         1923 g         1912 t
ORIGIN      
        1 cccaaacgag accatgcatg tcataaaggc caaatgcatt cttgtaggta gatcccaccg
       61 ccggaagcac ctccggcgtc gggtgatcat accattccag gatcagcctg gtcaggtctc
      121 gcgtcccctt cctacgaaca ggcgccgctc cggcggcata ttcccattcg gccattgtgg
      181 gaagacgttt acctttccac cggcaatagg cagtcgctgc aaaccaggag atattcgtca
      241 ccggactctt cgaaagacgc ggatcgccga tctccaggtc ccccgcccat tgcttcaggt
      301 agctgctgtc ggcaaaaatg cggactacct tcgaccgccg ccacccggga ttggccttca
      361 cgaattgcag gaattcgaca ttggtcacgg catgctcatc caggtagaaa ctccgcacct
      421 ttaccatctg tcgttggctt cccgtcagga taaatggctc aaaattccct ccgggtattc
      481 tcatcattcc cactggctgc gccgcaacca gcccccagcc gcacaacatc accagtatga
      541 tacacatggc tttcatgatc tcaccttatt ggttccttac tttttttact tccttctcct
      601 gtaccacacc ccccttattt ccccaactgt tccgaacata gctgagaata tcggcgatct
      661 ggtcgtcgga taaattcaat ttcggcatca caccctggaa gtgttttcca ttcacggtaa
      721 taggctcatt gttgataccg ttcagcaaaa cgccaatccc cttatccggc cttcccatca
      781 ggaaatccga cttcgacaac gggggaaagg cgccgggcac tccctgccct gtagactggt
      841 gacacgatga gcaattcatt tcgaaaagct catgcccatt cttaaatcgt acctccaagg
      901 gaagatcctg caccggcggt cctgcaggta catcgctttc ctctgcaacg gcagcacccg
      961 gctgatacaa ctcgtctttt atcttccccg aatagatatt tttgtctgcc gcaccggtca
     1021 cgaccagctg cgctacggcg cctttattga atgccctgaa aatggagtgg tccacgatat
     1081 tgtagacgcc cggctcgcga cacgtgaact ccacgatcgc cgcgcctccc gccggaatca
     1141 gcgtggtttg gacgttttga tgcacctggc tgccgccttc ctcatagaca ttgtcgaata
     1201 tctgcccgat aatatgaaaa gacgacacga gattcggacc gccattgcca acgaagatcc
     1261 tgatcctgtc ccctaccttc gcccgcagcg cgttgccgcc cgtcgcacct cccacagacc
     1321 cattgaacac cacatattcc ggctcctctt ttatcgcctt gtccatatca aacggctgaa
     1381 gacccccttc cccgttcttt cccttcgtat aaaactcgcc ctgcatcaga tagaactcct
     1441 tgtccacctt cggaaaccct ccctttggtt cgacaaggat catcccatac atcccgttgg
     1501 cgatgtgcat acccacagga gcggtcgcgc agtgatagac ataaagaccc gattttaatg
     1561 cccgaaagct aaaaacagac gtatgccccg gtaccgttag tgtggcctcg gcgcctccac
     1621 caggtccgga gactgcatgc aggtcaatat tgtgcggcat cttattgttg gggttattgc
     1681 tcaggtgcat ttccacctcg tctccctcct gtaccctgat catcggtccg ggtacgctgc
     1741 caccgaaagt ccagtaggta tatttcaccc catcggccag ctgcatcacc ttctccacca
     1801 cctccagtct cacgatcacc ttttcaggat gtgcataggt gggcgcaagc ggaaccgaag
     1861 gcgcatgcgc cagctcggca gtatattcgc cgatcacggc cgccgtatca tttgccgggt
     1921 tcgccggctc ggcagaatgt ttacaactga ttgtacaaag aaggatcaat gccagtccag
     1981 caggccccag cctcgcagca cctaaaccga atttttgaag aacgaaactc tttttcatgt
     2041 tttagcagat tgacaggtag cagatattgc gatgtcaaaa ctagctgccg ccgtctgccg
     2101 cgcgactgac gtccctcaaa ccaaaacatg atttttgtca ggtttcaaaa aaacacactc
     2161 attttcacgc aaataaacac aggctcctct ccgttcaaaa gaaaacgctc attttaagcc
     2221 ggataaagaa cggggacccc ggagtaaagc tgatatcatc caccgcagca ggttccccct
     2281 tcatccgcga aaacgtctca aactgcgcct ccctccattt cgtcttcagg agattctgta
     2341 tctctaaacc cgtctcccac cgggaacagg tgtaattcac catcaggtca ctcacacaat
     2401 accccttggc caccagcgta ttgtccacat tggcgggccg atccttcata taccgataac
     2461 tcaatcctcc attcaaccca ttgcgcaccc gaaaatccaa cccacccgtg ccgcacagcg
     2521 ggacagccag cggcagataa ttcgcgcttt tcgccgcctg cagatcgcgc gctttgcaat
     2581 aattgacatc ggcatgcgcg aacagccagg acgccagctg gtaacgcgct gaaagatcga
     2641 tgcccttccg caatgtccta tcccccggat ccatggtccc ttcatcggcg tcataggtaa
     2701 actcctgctg cagcgacaag gtccacaccg ccgcattcaa cagcaaccgg ggcagtggtt
     2761 tccagtacac gcccaggtcg gccccatacg cagccggcag cacatcgagc cccctgttcg
     2821 cgatgacgac ccgcgcatcg ttggagtgaa atcccttccc cgcccggaaa tagaattgca
     2881 gcaacgggct gaccgtgtag gagacattga acttcgggct gacgacccat ttttcccggg
     2941 gtgcctgcca tgaagccagg tcatcacgat accggaaata caaccagtcc gcccggatac
     3001 ctacattgaa catccaattc ccccaatgat agttctcatc cagccagcca ctcgcagcat
     3061 attcgcgcgg aactcccgac tgcaagtctt ccatcacgtc attttcattc cgggtatgac
     3121 tcaacctgct aagcctgata aaattcgcct gcgaaccgat acctgcagcc gataacaact
     3181 cgccgccatc ggaaaaatac gcatgatgcg aaagacgccc gttgtagccg gccagatccc
     3241 gttgctcctg ttgtcgcagc tgatcaccat taacactgtc gtcggcaaaa aaagttggat
     3301 catagtgcag gctgaaataa tagtgtgaca gataaacctg gtgttccatc agccacccga
     3361 cacccatctt tgtcttcagt cgcacgatca aattggttcg tcccgtattc ccaccttgcg
     3421 cgctgtcgac atatccaaac cggccgatca gtccgtcctt cacagcccgc tccgggatct
     3481 cgccagaaga gcgccaggcg ccttcatatg tagaaaacgt cgcggtcagc cgggtcgcag
     3541 cagaaaggtt tacattgtat ttcccaaaca gactcagcct ccgcaaccgc tgcggcgaat
     3601 cgaaaggacc gtcggtatag gccgcttctc ctgccagata agcgcattgg tcatgagaca
     3661 tcgtccgccc atccggcaga cgaagcattg ccaacaccct ccctgtatga aacgcccctc
     3721 cttccagttt tacctcattc ttatcgagca catcggccgt ccggaacgcc acgtatcccg
     3781 ccgtagcaaa atctccatac tccgcataat atggcccctt cccataatcg aacctcgaca
     3841 cgagctccgg tatcaggaaa tgcatatctg cgaaaccctg gccatgaata tgggatacca
     3901 ggttgagtgg catcccgtcc accgaaagat tcacgtccgt cccatgatcg gcgtcgaacc
     3961 cgcggtagaa gatatgttcc gccagccccc cgccctggtg ttgcccaatg aaaagaccgg
     4021 gtaccagccg gagcaggtcc tgcgcggagc ttacaggccg caaccccaaa tccacctttt
     4081 ccaggatgtg aaaagacgca ttgggcgcag ggttgatcgt tatggtcgtc agatcgggcg
     4141 cctgccggat catcgggtac tccccggccc gcttacaaac tccttccggg aaacaccctc
     4201 catccacgca acaccctccg tccgcacaac aggcagccgc acagacgcaa tatgaccgat
     4261 agcccacaaa ggaaatgttc agcgaatccc ccctgtcacg aatggtcatc cggaacatcc
     4321 cctgcttatc cgtcaatacc gaatggacag gacggcccat ggcggaatcc gaaactgccc
     4381 gtatactgac gaccgcgcct tccagcggct cgcgcgtgtc tttatcgatg acacgtccct
     4441 gatgcaccac ctgcccgcgc acgccgtcca ccaaaagaaa acacaacgcg atccataagc
     4501 aacacttcac agccgtccgt ttcattcaga cgccgtttta tccagcaaag caagtgcctt
     4561 ggggaaaaga atattatttt caagatgaat atgctgttga agatcgaggt cgaattctcg
     4621 cagcttctca tagagaaacc gataggaatt gcaggcatcc tcaggcactg aatatccatg
     4681 cgtcatgacc ctgaaacctt ccaggtcttc ccccgaaatg ctgtgttcat tctgcatttg
     4741 cagaatggca tgccgcacga atgaaaggtt acccgcgccg gcctgttccg ctgtttcagc
     4801 cagttcttgc acagcgggaa acaaaacttt ttcctcccgg tccatgtgac ccaacagatc
     4861 cagcacagtt tgccggacgc gcagttccag gccgatcaat tcggaatggg cagcaccgtg
     4921 gtgcgcagcc acttttcggg catattccgt cagcaccggc gctgtctccc ggacgtattc
     4981 gtgatgacgt cttacgatat attctgccaa tttccccgtc ggccattgtt gaaaatcttc
     5041 ttcgggtgtc cgcacacgga gttcagccag atcgagcgaa gatctcagct cgccctccga
     5101 tattccggcg gaatgacagg cttgcccgat cgtctggccg ccgccgcagc aaaagtcgat
     5161 accgaatttc cggaaaacct cggctttccg atagtctttc cccgccagct ctcctatggt
     5221 ctgctccggg gtgaggttag gtacattcgt gttcatagtt ttgttattta taagtcttta
     5281 tgattgaatc ttcgcatcgc cgccatcagg ggaacaatca tccaaagcaa tagtatcacg
     5341 agcgccagca acgcccctcc gacactccca aaaaaggagc ggaacaccgc gccggtataa
     5401 cccatcatag cggaattatc cagttgcagc agcagcaata tccgggacag atcgatgggg
     5461 ttcagacagc ccagcacgat cgccggtttc tccaacgggt aatcgctgaa ctggaacaac
     5521 aggaacagta ccagcgcatc atatagcacc gaaaaataga accacaataa gatcgcgaaa
     5581 ccaatccccc tcgccttgtc tcgcatcagc accgatccca gcaccccaac cgctgaaaaa
     5641 atgagtgtca gcgccgaccc cgagactacg agcagcacgc cggtcacatc gtggaggtag
     5701 aataacaccg gtatccctac tcccacccca aacgcgataa gtaacgaaaa cgccagcccg
     5761 gcatacagac tcagcaatat cctgacgcgc cgcagcggct gggcgaccag tagttcgatg
     5821 aattcgacgc tgttataata atagaccgtc gaaaaaatga cgctcatcag cggcaccacg
     5881 atcagcacga tgttcagcag gctcagtagc gccttggcgc tattgtcctc cagattcagc
     5941 acggcgagag atatcccaag taataccacc gcgtaactca tgaccatcct gttgcgaagg
     6001 atatccagca atacatattt gacgatctta cgcatagtat ttcgcttgtt ataaatggct
     6061 tttctgttga ctccctgtta tttgcatata ttgcgcaata gcgcgcacga gtttctcttc
     6121 gcccgtatca gcccgtagcc gcgacaacgt cttatggaat ttcacgcgtc cctcctgcag
     6181 gtatactacc tcgctgacca tatcgtccag gtcggcgagg atatgggaag tgatcaatat
     6241 cagccggcct tcctcgcgga cggcctggat cttctctttc aatatgcccg tcgcgacagg
     6301 gtccaatcca gccgtaggct cgtcgagtat cagcaccgag gggttgaaaa gaaaagccag
     6361 tgacgcactc accttttgcc gcgtgccacc cgaaagcgtg cgcatcctct tgcctgtcat
     6421 accgtccagc ccgaaggcat gaaaaagttc ctcgtccaga ctccgtccct ccttcgcgca
     6481 actacccgcc cccctgtctc tgtttgcctc cacaacccat cgttcctcct tcgccatgcg
     6541 tatttccttc atcatcttga aaacctgtcc tatcgtcata ttatccgggt atcgtcctat
     6601 ctgtggcata taccccatat cggcgcgata agcccagccc tggagaatgt cctgcccatt
     6661 gaaccggatg ctccccttgt cgggcttgac catccctagc agactccgga tcagtgtagt
     6721 ctttccgctg ccattgggtc cgatcagcgc aatgctttgc ccctgggaaa gcgtaacact
     6781 cacgtcatcc agcaccgaca gccgtccaaa ttttttgcat atgtgtgaaa tttcgatcat
     6841 aaggctaatg gtttcatgag tggccgacga tcgaccagat tcaccggcgt aatactggga
     6901 accaccttct ccgtccggtc aagcagactg accatcaggc tatggtaaag catcatcacc
     6961 gccgggttct tttcggcgat cattgcatag aggctcaccg gatgataagg aatatctccg
     7021 atcccatcgc gatcgatatc atacccttca tatttgtccc aataattccg gtcataaaaa
     7081 ttcaatacca gcgtcccgtt cgtggcaatg tcaaaggtat ttccgacgaa attgttggcg
     7141 ctgatcgtgt cctccgagca actggcctgc accttgatcg cccatccgtt ccccgaaaaa
     7201 aggttccggt ttatctgtac gcgtgtggtg ccttccatat agatggcgat ggtattgtga
     7261 aggaaacgat ttgactctat tctgccgtca ctgatctcct ttagcagaat accataagcg
     7321 ccgccgccga gattgtccgc gaagacattt ccccgcatat ctactccatg cgagaacatg
     7381 acggacacac cggcctcatt gtgccggaag gtattcccct catatgcatc attatgcgag
     7441 aacatgaaat gaagcccgta tcgcacatta taagcactgt tattgcctcg gatcagtgac
     7501 tccgtcacga attcgaaata gatgccgtcc cgatgccggc ttatcgtatt gtccagaata
     7561 cgcatgtgac tgcaatgcca gcagtgtatc ccgttgcccg cctggccgct catcgccgcc
     7621 cccgaagtct ggccgcctac cgccgccccc aaagtctggc cgcctaccgc cgcccccgaa
     7681 gtctggcccc ccatcgccgc ccgcgacacc aggatattcc cgctcagcac acagctgtca
     7741 gaattctggc aatacaggcc aaaaaaagta tcgtcaagcc ggttcgacaa cactttcact
     7801 cccctaacac cggccagatg gatacccgcg atatcgttcc aaccggaata gcccgagtgc
     7861 cgcaacacaa atccctgaaa agtgacccca tccgccttca cgaggacgat ctcatagcgt
     7921 ccctctccat caagcacagg catcccaatc ccaatcagac agagacgctt gtcaatcacc
     7981 agcccctttt ccagatagac acccgcaaac accttaatgg tatcccccgg cgacgctgat
     8041 cgaacggccg ctccgatgct attcaacccg cctgtcttcc gtacttccca cgtcttcgcc
     8101 agaccacagg aaggccagac cagcccgagc aggattatgg atacaactct ttccatgtga
     8161 acgcttttcc ttccgaatga atgatttgtg catcacgcgc cgcggcatcg gcaaacgcgg
     8221 caatattccc gttcatgggt gtatggaaat tttcactgta taacaggaaa gcatccgcgg
     8281 cctttatcca gggtcctcca ttatagtcga caaggtaaat accggcaaca tcccctgcag
     8341 ccggcacacc cgtcttcaaa gacgtcacca aacagtggac gtcatcgaac ttatacacct
     8401 tccccttttt cgaaatcacc tctcccccga aatgccggtc ggtaaacgcc atcttgcaga
     8461 aatcgcaatt gtcctggcca taccgaatgg cctgcagacc ggacccgcag gatgacaggg
     8521 caacaacgac cgcaagcgcc accagcacgg gcgcccccgc acccgaatac agacgccgaa
     8581 ccttatgccc ctttctccat tccaacaccg caactcccac cagcaccacg cctaccgcaa
     8641 cgaagatcca gccacccaga tccggaatgc tgtaggcccc gaaattgagc agttgct
//
LOCUS       DASYSV010000043         5535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1291700, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000043 DASYSV010000000
VERSION     DASYSV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291700"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VGQ51_01310"
     CDS             <1..754
                     /locus_tag="VGQ51_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035219.1"
                     /translation="AQAHIDQVYSHADTTLYVDRMDHLYVEKDNSFFADGNRIIGGPL
                     VQQGKGEAQVCCFGGQYAGLWVDNNASFTAKDCWWEGAARIPLYLRGSGKITIDGTMI
                     APLHADSLPTIRIGSFNGDVSLLNMYVQGAFLPDAANPGLNLLVWNVHCYYKMNALDF
                     IGKGGSWKGAFLGINTQCFNANDPSCKSIASIPDATHNIGDINPFLDALTAQDRSTEP
                     RMLRNLPAGVSNIYISRVTLGNMQEGILFSKE"
     gene            756..1892
                     /locus_tag="VGQ51_01315"
     CDS             756..1892
                     /locus_tag="VGQ51_01315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV9035220.1"
                     /translation="MKKILLFIDWFEPGYKAGGPIRSCVNFVRSMHRHYKIYVFTSDR
                     DLGSTDAYPDIPVDRWIAGQDQVELFYCSPENLRWSTIRRELDSIRPDFLYLNSMFSL
                     HFTIYPMLINRRRRNPARVILAPRGMLKSTAVQFRPLKKKIFLKSWRWLGLHRPIHFH
                     ASDNTEVNDTHRYFGTRVRVTQIPNFPSIFPERDRPLEKKPGELSIIFVGRVHPIKNL
                     DYLLNALKGLPDCLRLTIIGSLEDKPFWEKCQQIITELPPTTRVVYAGEKPHHELPPI
                     IGEHHIFALPTQGENFGHAIFEAFALGKPVLISDQTPWRNLEKSGVGWDLPLDRPDLF
                     RQAIRTAASFSQNEYDKWSRSARRYSQDYIEKMNLADDYCTLFS"
     gene            1901..3070
                     /locus_tag="VGQ51_01320"
     CDS             1901..3070
                     /locus_tag="VGQ51_01320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV9035221.1"
                     /translation="MKKLAIVTSHPIQYNAPLFKLLTEHGRLSVRVFYTWGQSSQGKK
                     YDPHFGKDIDWDIPLLEGYDHVFVENIAKDPGTHHFKGIVNPTLNREIEGWKPDAVLV
                     FGWSFTSHLRCMRHFYGKVPVLFRGDSTLLGEQPGLKRLMRRIFLKWVYRHVDYALYT
                     GSHNKEYYLRHGMREDQLVFAPHAVDNDRFGEPDADHRARAIEWRHRLGIADDHVVIL
                     FAGKLEPRKNPFFLTELAHRIGNDKIRIVITGNGPLEASLKDAAGAGSRVLFLDFQNQ
                     AIMPVLYRIGDVFVLCSGSETWGLGANEAMASGCALMLSDKTGGAVDLVKEGVNGIIF
                     GLTDYDKCREFAETLVNHPQRLADMKKASRRLIGGFSYARIVGAIETLLLKQDRQ"
     gene            3070..4800
                     /locus_tag="VGQ51_01325"
     CDS             3070..4800
                     /locus_tag="VGQ51_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV9035222.1"
                     /translation="MYILGLNAYHADSSAAIFKDGKMIAATEEERFRRVKHWAGFPSM
                     AVKFCLQEAGIGLSQVDHIAIGRDPYAKLGKKLLFLASHPGGGWSAVKDRLNNAKKVA
                     SLETELTAIDPSVDARLLKSRIHQVEHHRSHLGSAFFASPFDEAALLSIDGSGDFSTA
                     MIGTGKGNKITILDSVDFPHSVGLFYTAFTQLLGFPHYGDEYKVMGLAPYGKPEYVDR
                     LRDVLVFLPNGLFRLNTKYFRDARSGIISYGEDHIPVVAPLYSDYMVEKFGAPRKKEE
                     ALTQYHKDMAASVQRITEELIFHILTHLHKKTGLENICIAGGVAQNSVANGKLTRNTP
                     FRNVYIPSAGHDAGISMGSALYVYNHVLDQPRAEPVWSAYTGSRFSNETIEKYLQSRN
                     ISYRVFPDDQLYETVADRLIDAGVIGWFNGRAEFGPRALGARSILADPRRSDAKDLLN
                     SKIKRRESFRPFAPSILKEYIPEYFEVTDAVPFMEKVFPIREEKRASIPAVTHADGTG
                     RLQSVDKIVSPRYYRLIEAFYKKTGVPILLNTSFNENEPIVNSPEEALECFLRTNMDM
                     LVLENCVITR"
     gene            4805..>5535
                     /locus_tag="VGQ51_01330"
     CDS             4805..>5535
                     /locus_tag="VGQ51_01330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9035223.1"
                     /translation="MKISIITATYNSAATVKDTLTSIREQDHPDIEHIIIDGGSADAT
                     AAIVSGFPHVSKWVSEPDKGLYDAMNKGIRLAHGDVIGILNSDDIYADASVLSDVARV
                     FEDPAVMTVYADLHYVQHDDMARVKRVWHSGPFKRNYFYYGWMPPHPTFFVRKEVYEK
                     AGGFNTSLRTSADYELMLRILLKYGLSTRYIRRVTVKMRVGGVSNATLRNRFRGNRED
                     RLAWKLNDLKPYFFTLYLKPLRKIT"
BASE COUNT         1306 a         1564 c         1465 g         1200 t
ORIGIN      
        1 ggcgcaggct catatcgacc aggtatattc gcatgccgat accacattgt acgtggatcg
       61 gatggaccac ctttacgtag aaaaagacaa ttcctttttt gcggacggca atcgtatcat
      121 cggcgggccg ctcgttcaac aaggcaaggg agaggcacag gtctgctgtt tcggaggcca
      181 gtatgccggt ctctgggtgg ataacaatgc cagcttcacg gccaaggatt gctggtggga
      241 aggcgccgca cgtatcccgc tttacctgcg gggctccggc aagatcacca tcgacgggac
      301 aatgatcgct cccctccatg ccgacagcct gcccaccatc cgcatcggtt cgttcaatgg
      361 cgacgtttcc ctgttgaata tgtacgtgca gggcgctttc ctgcccgatg cggccaaccc
      421 ggggttgaac ctgctggtat ggaacgtaca ctgctattat aaaatgaacg cgctggattt
      481 catcggtaaa ggcggttcct ggaaaggagc attcctgggt atcaacaccc aatgtttcaa
      541 tgccaacgac ccttcctgta aaagcatcgc ttccattccg gatgccacgc ataatatcgg
      601 cgatataaat ccgttcctgg atgcgctgac tgcacaggac aggagtacgg aaccacggat
      661 gctccgcaac ctgccggcgg gcgtcagcaa tatctatata tcccgcgtga cgctgggaaa
      721 tatgcaggaa ggcatcctgt tcagcaagga gtagcatgaa aaagatcctt ttattcatcg
      781 attggttcga acccgggtat aaagcaggcg gtcccatccg gtcctgcgtc aacttcgtgc
      841 ggagcatgca ccgacattat aagatctatg tctttacttc cgaccgggac ctgggttcta
      901 ccgatgccta tccggatatc cctgtcgatc ggtggatcgc tggtcaggac caggtcgagc
      961 tgttctactg ctctcccgaa aacctgcggt ggtcgacgat tcgccgggag ctcgattcca
     1021 tccggccgga tttcctgtat ctgaacagca tgttctcgtt gcatttcacc atctatccga
     1081 tgctgatcaa ccggcggcgc cgcaaccccg cccgggtgat ccttgctccg cggggaatgc
     1141 tgaaaagcac cgccgttcaa ttccggccgc tcaagaagaa aatatttttg aagagctggc
     1201 gctggctggg tcttcatcgc ccgatccatt tccacgcctc ggataatact gaagtcaacg
     1261 atacacacag atatttcggc acgcgcgtcc gcgtgaccca aatacccaat ttcccaagca
     1321 tatttccgga gagggatcgg ccgctcgaaa aaaaaccggg cgagctgtcc attatattcg
     1381 taggccgtgt acatccgatc aaaaatctcg actatctgct caacgccctc aaaggactac
     1441 cggattgtct tcgactgacc attatcggaa gtctcgagga taaacctttc tgggaaaaat
     1501 gccagcagat catcacggaa ctgccgccga ccacccgtgt cgtctatgcg ggcgaaaaac
     1561 cgcatcacga gctgcccccg atcatcgggg aacatcatat tttcgccctg ccgacacaag
     1621 gagagaactt cgggcacgct atctttgaag cattcgcgct gggcaaaccg gtcctgatca
     1681 gcgatcagac accctggagg aacctcgaaa agtcaggagt gggttgggac ctcccgttgg
     1741 accggcccga tctttttcgt caggccatcc ggacggccgc ctccttctca cagaacgagt
     1801 acgataaatg gagccggtcc gcccgccgct attcacagga ttatatagag aaaatgaacc
     1861 tggcggacga ctattgtacc ctgttcagtt aacccgacgc atgaaaaaac tggccatcgt
     1921 tacatcgcac cccatccaat acaacgcgcc cctgttcaaa ttattgacgg agcacggacg
     1981 cctcagcgtc cgcgttttct acacctgggg tcagtcctcg caaggaaaga aatacgatcc
     2041 gcacttcggc aaggacatcg actgggacat tcccctgttg gaaggatatg accacgtatt
     2101 cgtcgaaaat atcgccaaag accccggcac ccatcatttc aagggtatcg tcaacccgac
     2161 gctcaaccgg gagatcgaag gatggaagcc ggatgccgta ttggttttcg gatggagctt
     2221 caccagccat cttcggtgta tgcgccattt ctacggtaag gtgcccgtcc tgttcagggg
     2281 agactccacg ctgctcggcg aacagcccgg cctcaaacgg ctgatgagaa gaatttttct
     2341 gaaatgggta tacagacacg tcgattacgc gttgtatacc ggttcgcata acaaagagta
     2401 ttatttgcgt catgggatgc gcgaagacca gctggtattc gccccgcacg ccgtcgacaa
     2461 cgaccgcttc ggagaacctg acgccgatca ccgcgcgagg gcgatcgaat ggcggcatcg
     2521 gttgggcatc gccgacgacc acgtggtgat ccttttcgcg ggaaaactgg agccgagaaa
     2581 gaacccgttc tttctcacgg aactggcgca ccggatcggc aacgacaaga tccggatcgt
     2641 catcacgggc aacggccctc tcgaggcatc cttgaaagat gccgccggtg ccggttcacg
     2701 ggtgctgttc ctggatttcc agaaccaggc gatcatgccg gtgttgtatc gtatcggcga
     2761 tgtattcgtg ctgtgctcgg gcagcgaaac ctgggggctg ggcgcaaatg aggccatggc
     2821 cagcggctgc gcacttatgt tgtccgataa gacgggcggc gcggtagatc tggtgaagga
     2881 aggcgtcaat gggatcatct tcggtctgac cgactatgac aaatgcaggg agtttgcgga
     2941 aacgctggtt aatcacccgc agcggctcgc cgatatgaaa aaggcttcga gacgactgat
     3001 cggaggtttt tcctatgccc ggatcgtcgg ggcaattgaa acactactac tcaaacaaga
     3061 tcgtcaataa tgtatatcct aggactaaat gcttatcacg ccgactcttc ggcagcaatt
     3121 ttcaaagacg gaaaaatgat cgccgccacg gaagaagaaa gattccggag ggtcaaacac
     3181 tgggcgggct tcccgtcgat ggccgtgaag ttctgtctgc aggaggccgg catcgggctt
     3241 tcgcaggtgg accacatcgc catcgggcgc gacccgtatg ccaaactggg gaagaaactc
     3301 ctgttcctgg ccagccatcc cggaggcggc tggtctgctg tcaaagatcg tcttaacaac
     3361 gccaaaaaag tcgcttccct ggaaaccgaa ttgaccgcca tcgatccttc agtcgatgcc
     3421 cgtctgctga aaagcaggat acaccaggta gagcaccatc gcagccatct cggcagcgct
     3481 ttttttgcca gcccttttga tgaagcggcg ttgctcagca tcgacggctc gggggatttc
     3541 agcaccgcga tgatcggcac gggtaagggg aataagatca ccatcctcga ctcggtggat
     3601 ttcccgcata gcgtaggact attctatacg gcattcacac agctgctcgg cttcccccat
     3661 tatggcgatg aatacaaggt catgggactc gctccctatg ggaaacccga atatgtcgac
     3721 cgtctgcgcg acgtcctggt attcctgccc aacggcttat tccgtctcaa tacgaaatat
     3781 ttccgcgatg ccaggagcgg gatcatctcg tatggcgaag accatatccc cgtggtggcc
     3841 cccctgtatt cggactatat ggtggaaaaa ttcggcgcac cccgcaaaaa ggaagaggcc
     3901 ctgacccagt atcataaaga tatggccgcc tcggtacaaa ggatcaccga agagctcatc
     3961 ttccacatcc tgacgcacct gcacaaaaag accggtctgg aaaatatctg tatcgcaggc
     4021 ggcgtcgccc agaactccgt tgccaatggc aagctcacgc gtaatacacc cttcaggaac
     4081 gtgtatatac cttccgccgg tcatgatgcg ggtatctcga tgggctcggc gctgtatgtg
     4141 tacaatcacg ttttggacca gccccgcgcc gaaccggtat ggtcggccta taccggtagc
     4201 cgtttcagca atgagacgat tgagaaatat ctccagtcgc gcaacatatc ttaccgcgta
     4261 ttccctgatg atcagctgta tgagacggtt gccgaccgcc tgatcgatgc gggagtgatc
     4321 ggctggttca atggaagggc tgaattcggt ccccgcgcac tcggcgccag atccattttg
     4381 gccgaccccc gccggtcgga cgccaaggac ctcctcaaca gcaagatcaa acgccgcgag
     4441 agcttccggc ctttcgcccc ttccatcctg aaggaatata ttcccgaata tttcgaggtc
     4501 accgacgccg ttccattcat ggagaaggtg tttcccatac gcgaggaaaa acgcgcaagt
     4561 atacccgccg ttacgcatgc cgacggtacc ggcaggctgc aatcggtgga caaaatcgtg
     4621 agcccccgtt actaccggct gatcgaagcc ttctacaaaa aaacgggtgt gcccatcctg
     4681 ctcaacacct ccttcaacga gaacgaaccg atcgtcaact cgcccgaaga ggcgctcgaa
     4741 tgtttcctgc ggacaaatat ggacatgctg gtattggaga attgcgtgat cacccgctag
     4801 tcatatgaag atatcgatca taacagccac gtataatagc gctgcgacgg taaaggatac
     4861 gctgaccagc atccgggaac aggaccatcc cgatatcgag catattataa tcgatggcgg
     4921 atcggccgat gcgacagccg ctatcgtctc tggtttcccg catgtgagca aatgggtttc
     4981 ggagccggac aagggtctgt atgacgccat gaacaaaggg atccggctgg cacatggtga
     5041 cgtgatcggg atcctgaatt ccgatgacat ctatgcggac gcctcggttt tatccgatgt
     5101 tgcccgcgtc ttcgaagatc cggccgtcat gaccgtttat gccgatctgc attacgtgca
     5161 gcacgatgat atggcccgcg tcaaacgggt gtggcattcc ggtcctttca aacgcaacta
     5221 tttttattat ggctggatgc ccccgcaccc gaccttcttc gtccggaaag aggtgtacga
     5281 aaaggccgga gggttcaaca ccagcctgag gacatccgcc gattatgagc tgatgcttcg
     5341 tatcttgctg aaatatggcc tttctacccg atatatccgc cgcgtgaccg tgaagatgcg
     5401 ggtaggaggg gtcagtaacg ctacccttcg aaaccgtttc cggggcaacc gcgaggaccg
     5461 ccttgcctgg aagctcaatg atctgaaacc ctattttttt acgctttatc ttaaaccact
     5521 gagaaaaatt accca
//
LOCUS       DASYSV010000044         6024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1806719, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000044 DASYSV010000000
VERSION     DASYSV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806719"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VGQ51_01335"
     CDS             <1..901
                     /locus_tag="VGQ51_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035224.1"
                     /translation="KGEDATEQSFIINGVNFLLNSDVEGQQLPDRGAPNILITNGGTQ
                     LHNEFEDDGIYYWKFHVDWKDTSKTRLEGPLKIEVAPYHYAGDGQLKRSVPQPDTAMR
                     LDTQGDKVLGRLIYRRIGKQQSYIVVHSVRSAAGGSGIRWYEFRGAKHGALQLYQQGT
                     YAPDGDWRWLPGIATDKYGNMGIGYSFGGATHFPGQRFTGRLATDSLGVMGFGEGILI
                     EGQASQMNTLRWEDYTYTAIDPSDDETFWYVGDYLKKGAKNYSTRIGSFRLAGPGVAA
                     ARNQSSITAQAGRQKSINSQALK"
     gene            complement(868..2760)
                     /locus_tag="VGQ51_01340"
     CDS             complement(868..2760)
                     /locus_tag="VGQ51_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEV9035225.1"
                     /translation="MDYFTHLAGLLKTEQNEDKRLFNELTASASTAQRREAGVTWYPI
                     AIRDTELSRGDYLTVEIERTTHRDLAHQLRFGAQAALFSNHDPANDRVEGTIAFQSGD
                     RLKLTLRTDELPEWARDGKLGIDLLFDDNSYTEMFGALKQAASLAEKGPEGRLVRVLT
                     GVETPAFAQTTAAPSPGLNPGQQSAVDKILAADNLAIVHGPPGTGKTTTLVAAILALI
                     QRDGGPILVTAPSNTAVDLLSEKLADAGLYVLRIGNPSKVSARLQSLTLDSRMAEHVT
                     TKEIKKLKKRAAEFKDMAHKYKRNFGPAEREQRKALFTEARAILKEVERSEQYILNDI
                     LARAQVITATLVGANHYTIRHLKYSTTVIDEAGQALEPACWIPALKAPRLILAGDHCQ
                     LPPTVRAEDALNTTLLEKCVSLHPGSVTLLEEQYRMHTRIMGYSSEVFYDRRLRAHPT
                     VAGHTLAEGDAPLVFIDTAGCGFDESPEGTGMTNPEEAAFLVRHVTQLVPELDGRYSP
                     ADFPTIGIIAPYRNQVHLLQELVGATRGLRDHADKITVNTIDSFQGQERDIVYIGLTR
                     SNADSRIGFLSEIRRLNVAMTRARKKLVIIGDGATLSQLPFYEGLITYSQQHDAYFSA
                     WEFMDF"
     gene            2828..3160
                     /locus_tag="VGQ51_01345"
     CDS             2828..3160
                     /locus_tag="VGQ51_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV9035226.1"
                     /translation="MRRDVFQAIADPTRREIIALIADRSLNVNAVAGNFDVSRTAVYK
                     HMKILTECGLVVVRQQGRERFCEARLERLHEISDWVEQYRKFWNARFDALEDYLNKME
                     PKKKKGRG"
     gene            complement(3157..3822)
                     /locus_tag="VGQ51_01350"
     CDS             complement(3157..3822)
                     /locus_tag="VGQ51_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035227.1"
                     /translation="MKNLIFPALLCIGLLPARAQKIVEKHITFSPKNFVSMNFQISDS
                     IHIVTWKKDEVYIHSSIDVNDNQDNDDYKMVFDETGSTINVSAKLETDNTRRSKKDSG
                     NCCCCCNYRTKIYHEVYIPENADFSVETINGNIVINGNTAEIRAKSISGFIDLAIAPD
                     RKAEVKMHTITGTMYSNIDLPTSRHIKQVGGGSITAELNGGGGKEIELETISGNIFFR
                     KAG"
     gene            complement(3889..4668)
                     /locus_tag="VGQ51_01355"
     CDS             complement(3889..4668)
                     /locus_tag="VGQ51_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEV9035228.1"
                     /translation="MKKMKYSFLLVASLCIAGLCHAQEQYKVSLDNLKDAKLILENFR
                     DELTIEGYAGNDIIVTGGREERSPDRARGLKPIYGGGTDNTGIGVAMEKEGNRISLRC
                     LLPITHGSHFKIRIPESIALKITRDCMGGGDTYISDMKGEVEFNGCHDVTLKNVTGPL
                     VISTISGNVSVVFTEINKDKPISIASVSGEVDVTMPAKAGFNLEMGTISGNMYSDFDI
                     PSAKQGDMERVGGSNIHTKLNGGGIDLRLHSVSGNVYLRKG"
     gene            complement(4689..5513)
                     /locus_tag="VGQ51_01360"
     CDS             complement(4689..5513)
                     /locus_tag="VGQ51_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV9035229.1"
                     /translation="MNCKEVQTLMIDYLDRTTDPATSGRMRQHLENCDQCKHEAEELQ
                     QLLTSMRDTVLETPPPALRESFDTMLQSELNMLTTANIIEEFRPAGTQPGKIVPISLS
                     SPVWKVAAAVILVATGVVIGRNLPSGQQPIASANMDSLRSEIRSMRQEVMLNLIDDES
                     ASQRIKAVSYTEDISNPDQQVIDVLVHTLNNDKNVNVRLAALYSIARFADKRAVRDSL
                     VSSLPTQTEPIIQVVLINLLAEKRDTRAIAPIRNIITNKKTLKEVKDAAQRSLQSF"
     gene            complement(5523..>6024)
                     /locus_tag="VGQ51_01365"
     CDS             complement(5523..>6024)
                     /locus_tag="VGQ51_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV9035230.1"
                     /translation="VKEGHLAELTALFDRYQASLYNFFFRLTFDQAASQDLTQNLFYR
                     VIRYRQSYDPTQGSFRSWIYRMARNVHFDYCKQEQKVPGGKTDPSALEQADETTGFTQ
                     DQFQRLDEALAQLAPDQREVLVLSRYQGLKYEEIARIRESSVAAVKVQVFRALRQLRT
                     LYFKQH"
BASE COUNT         1206 a         1541 c         1815 g         1462 t
ORIGIN      
        1 gaagggcgag gatgcgacgg aacagtcgtt cattatcaac ggggtgaatt tcctgctgaa
       61 ttccgatgta gaaggacaac agttgccgga caggggcgcc ccgaatatcc tcatcaccaa
      121 tggcggtacc caactgcaca atgaattcga agacgatggc atttattact ggaagttcca
      181 tgtcgactgg aaggatacct cgaagacgcg tctcgaaggg ccgctgaaga tcgaagtagc
      241 gccttatcat tatgccggtg atggtcagct gaagagatcg gtcccccagc cggatacggc
      301 catgcgtctg gatacccagg gcgacaaagt attgggacgg ctgatctatc gccgcatcgg
      361 caaacagcag tcctatatcg tcgtccattc ggtgcggagc gccgcgggag gcagcgggat
      421 acgttggtat gaattcaggg gagcgaaaca tggtgcgctg cagctgtatc agcaggggac
      481 ctatgcgccg gacggcgatt ggcgctggct gccgggtatt gccacggaca aatacgggaa
      541 tatgggcatc ggttattctt ttggcggcgc gacacatttt cccggtcagc gttttaccgg
      601 caggctcgca accgacagcc tcggcgtgat gggtttcggg gaaggcattt tgatcgaagg
      661 gcaggcgtct caaatgaata cgctgcgctg ggaggattac acatataccg ccatcgatcc
      721 ctccgacgac gagacgttct ggtatgtggg cgactatctg aaaaaagggg cgaagaatta
      781 ttccacgcgg atcgggtcgt tcaggctcgc cgggcccggg gtcgcggcgg ccagaaatca
      841 gtcatcgatc accgcccagg caggccgtca aaaatccata aactcccagg cgctgaaata
      901 agcgtcatgt tgctgggaat aggtgatgag cccttcatag aagggcagtt gcgagagcgt
      961 ggctccgtca ccgatgatca ccagtttttt acgggccctc gtcatggcta cgttgagccg
     1021 gcggatctcc gacaggaaac cgatcctgct gtcggcattg ctgcgggtaa gcccgatgta
     1081 cacgatatcc ctttcctgtc cctggaagct gtcgatcgta ttgaccgtga tcttgtccgc
     1141 atggtcgcgt aagccccggg tggcgccgac cagttcctgc aacagatgga cctggttgcg
     1201 gtaaggagcg atgatgccga tggtcgggaa atccgcaggg gaatagcgac catcgagttc
     1261 gggcaccagc tgagtgacat gcctgaccag gaaagcggct tcttcgggat tggtcatacc
     1321 cgtcccttcg ggtgactcgt cgaagccgca accggcagtg tcgatgaaga ccaggggagc
     1381 gtccccttca gccagcgtat gtccggcaac ggtaggatgc gccctgagcc ggcggtcgta
     1441 aaatacttct gaagaatagc ccatgatccg tgtatgcatc cggtattgtt cttccaggag
     1501 tgtgaccgat ccgggatgga gggatacaca tttttccagc agggtagtgt tcagggcatc
     1561 ttccgcacgc accgtcgggg gcagctggca gtggtcgccc gcaagtatga gacgcggagc
     1621 cttcagcgcg ggtatccagc atgcgggctc gagggcctgg ccggcctcat cgatgacggt
     1681 ggtggagtat ttcagatggc ggatggtata atggttggcg ccgacgaggg tggccgtgat
     1741 cacctgtgcg cgggcgagga tatcgttcag gatgtattgc tcggaccgtt cgacctcctt
     1801 aaggatcgcg cgggcttcgg taaacaatgc ttttcgttgt tcgcgttccg ccggtccgaa
     1861 attccgtttg tatttatggg ccatgtcttt gaactcggcg gcgcgtttct tcaatttttt
     1921 gatctccttc gtggttacat gttccgccat ccggctgtcc agcgtcagcg attgcagtcg
     1981 tgcggatact tttgaaggat tgccgatgcg aaggacgtat aggcccgcgt ctgcgagctt
     2041 ttcgctgagc aggtccacgg cagtattgct gggcgccgta accaggatgg ggccgccgtc
     2101 gcgttggatc agggcgagga tggccgcgac cagcgttgtc gtcttacccg tgccgggagg
     2161 gccgtgtacg atcgcgaggt tatcggctgc gaggatctta tcgacggcgg attgttgccc
     2221 ggggttcagg ccgggcgatg gtgcggctgt cgtttgcgca aaagcgggcg tctccacgcc
     2281 cgtcaggaca cgcaccagtc gcccttccgg gcccttttcg gccagcgatg ccgcctgttt
     2341 cagcgcgccg aacatttcgg tatagctgtt atcgtcgaaa agcagatcga tccccagctt
     2401 gccgtcacgt gcccattcgg ggagctcgtc ggtgcggagg gtcagcttca gccggtcgcc
     2461 ggactggaaa gcgatcgtac cttcgactct gtcgttcgcg gggtcatgat tggaaaaaag
     2521 tgccgcctgg gctccgaaac gcaattgatg ggcgaggtcg cggtgtgtgg tccgttcgat
     2581 ttccacggtc aggtagtctc cacggctcag ctcggtgtcg cggatagcga tggggtacca
     2641 ggtgacaccg gcttcgcggc gttgggctgt cgatgcggat gcggtcagtt cgttgaacag
     2701 gcgtttgtct tcgttttgct cggttttcag gagtccggcg agatgcgtga aataatccat
     2761 ccggcaaatt taaaatttgc cggtcacaag gaataaccta ttttgcaacc aaaaggtttc
     2821 attttatatg cgtagagatg tattccaggc gatagccgac ccgacgcgca gggagatcat
     2881 tgcgctgatc gccgaccggt cgttgaacgt caatgccgtt gcggggaatt ttgacgtcag
     2941 ccggacagcg gtatacaaac acatgaagat actgactgag tgcggtctcg tcgtcgtccg
     3001 tcagcagggc agggagaggt tttgcgaagc gagactggag cggcttcatg agatcagcga
     3061 ttgggtggag caatacagga agttctggaa tgcgcgtttc gacgccctgg aagattatct
     3121 gaacaagatg gagccgaaaa aaaagaaagg caggggttaa cccgcctttc tgaaaaagat
     3181 attgccgctg atggtctcga gctcgatctc ttttccgccg ccgccgttga gctccgcggt
     3241 gatcgagccg ccgcctacct gtttgatatg ccggctggtg ggcagatcga tattcgaata
     3301 catcgtaccg gtgatggtat gcatcttcac ctcggctttc cggtcggggg cgatggccag
     3361 gtcgatgaag ccgctgatac tttttgcacg gatctcggcg gtattcccat tgatgacgat
     3421 gttgccattg atcgtctcca cggagaaatc ggcgttctcg gggatataga cctcgtgata
     3481 gatcttcgtc cggtagttgc agcagcaaca acagttaccg gaatcctttt tcgaacggcg
     3541 ggtattgtcg gtctcgagtt tggcggagac attgatggtg ctgccagtct cgtcgaacac
     3601 catcttgtaa tcatcgttat cctggttatc gttcacatcg atcgagctgt gaatatacac
     3661 ctcgtctttt ttccaggtca cgatgtggat ggaatcggag atctggaagt tcatcgagac
     3721 gaaattcttc ggcgagaagg tgatatgttt ttcgacgatc ttttgcgccc gggcgggcaa
     3781 caggccgatg cacagcagtg caggaaaaat gagatttttc ataaacgctg gtttgtatga
     3841 ttagtttgtt gttggaaaaa ccctggtaga cgatgtgagg gcaggcgcct agcctttgcg
     3901 caggtagacg ttgccgctga cgctatgaag ccggagatcg ataccgccgc cattcagctt
     3961 tgtgtggata ttgctgccgc cgacgcgctc catatctcct tgcttagcgg atggtatatc
     4021 gaaatccgaa tacatgttgc cggatatggt acccatttcc aggttgaagc cggcttttgc
     4081 gggcatcgtc acatcgacct cgccgctgac ggaagcgatg gagatcggct tatctttatt
     4141 gatctcggta aatactacgc tgacattgcc gctgatggtc gaaataacga gcgggcccgt
     4201 gacattcttg agtgtgacgt cgtgacaccc gttgaattcg acctcacctt tcatatccga
     4261 gatataagtg tcgcctccgc ccatgcaatc ccgggtgatc ttcagggcaa tgctttcggg
     4321 tatcctgatc ttaaaatgcg agccatgcgt gatgggcaac agacagcgga gagagatgcg
     4381 gttgccctcc ttttccatag cgaccccgat gccggtatta tcggttcctc cgccatagat
     4441 gggtttcagt cccctggcgc ggtcaggaga cctttcctcc ctgccgccgg tgacgatgat
     4501 gtcattgccg gcataacctt cgatggtcag ctcatcgcgg aaattttcca ggatcagttt
     4561 ggcatctttg agattgtcga gcgagacctt gtactgctcc tgggcatggc acagacctgc
     4621 gatacatagg gacgcgacga ggaggaagct atacttcatt tttttcatgt tgttttatat
     4681 ttaaaaattt aaaaagattg gaggctgcgc tgggcggcgt cttttacctc tttgagggtt
     4741 tttttgttgg tgatgatatt ccggatgggt gcgatggctc tcgtgtcgcg tttttccgcg
     4801 agcagattga tcagcacgac ctggatgatc ggctcggttt gcgttggcag cgagctgacc
     4861 agtgaatcgc gtacggcgcg tttgtcggca aaccgggcaa tcgaatacag ggccgcgagt
     4921 ctcacgttga cgttcttatc attgttcagc gtatggacca gtacatcgat gacctgctgg
     4981 tcgggattgg atatgtcttc ggtatagctg accgccttga tccgctggct ggccgattcg
     5041 tcgtcgatca ggttgagcat gacttcctgc cgcatcgatc ttatctcgct gcgcaatgag
     5101 tccatattgg cagaggcgat cggttgctgg ccggatggca ggttcctgcc tatgacgacg
     5161 ccggtggcta ccaggatcac cgcagcggcg actttccata cgggcgatga cagtgaaatg
     5221 ggaacgatct ttccgggctg tgtgcctgca ggcctgaatt cctcgatgat attggccgtc
     5281 gtcagcatat tgagctcgga ttggagcatg gtatcgaaac tctcacgcaa agcaggcggc
     5341 ggcgtttcca ggaccgtatc gcgcatcgag gtcagcaact gttgcagctc ttcggcctcg
     5401 tgtttgcact ggtcgcagtt ctcaagatgt tgtctcatcc tcccggatgt ggcgggatcc
     5461 gtcgtccggt ccagatagtc gatcatcagc gtttgtactt ctttacaatt catggtcacc
     5521 gcttaatgtt gtttaaaata gagggtcctc agttgtcgca aagcccggaa cacctgcacc
     5581 ttgaccgccg cgactgagct ttccctgatc ctggcgatct cctcgtattt cagaccctga
     5641 taacggctga ggacaagcac ctcgcgctga tcgggcgcca gctgtgccag cgcttcgtcg
     5701 aggcgttgga actggtcctg ggtaaatccg gtagtctcat ccgcctgctc cagtgccgaa
     5761 ggatcggttt tgcctccggg taccttttgc tcctgtttgc agtagtcgaa atgtacattg
     5821 cgcgccatcc gatagatcca tgaccggaag ctgccctgcg tcggatcgta gctttgccgg
     5881 taccggatga cccggtagaa caggttttgc gtcaggtcct ggctcgcggc ctggtcgaat
     5941 gtcagccgga aaaagaaatt atagaggctg gcctggtacc ggtcgaacaa tgctgttagc
     6001 tcggccagat gcccttcctt tacc
//
LOCUS       DASYSV010000045         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1547799, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000045 DASYSV010000000
VERSION     DASYSV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547799"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VGQ51_01370"
     CDS             <1..432
                     /locus_tag="VGQ51_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEV9035231.1"
                     /translation="GLIHYGSAWHLIGWCHLRNDYRDFRLTRMLGVNLEEITFDPSSH
                     PSIREYIDRLRAGADLQEVIILVDRRIVKYLQEQKYLNGFVSQDELEDCMRMKFLVSS
                     LDYFARWLLMYTDAIRVETPRELQDRMQKISGQLSEHHGSY"
     gene            544..1110
                     /locus_tag="VGQ51_01375"
     CDS             544..1110
                     /locus_tag="VGQ51_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV9035232.1"
                     /translation="MNTRTCAAFIFDGFSDHELAITVAGLHRRDNIVLETFANKGRTA
                     TSRTGLRTIPHTSLSYMSPDDFDILVLPGGQQWEKGDNLEIFPLITATAGRRPIVAIS
                     EATLALADLGLLNDIPHTGIHPDHFERFCPDYEGASYYKSQPCIDAGMIITVDGMAIT
                     QSGHGMLCLFDTLQNIIAPHHELFDPAC"
     gene            1085..2041
                     /locus_tag="VGQ51_01380"
     CDS             1085..2041
                     /locus_tag="VGQ51_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEV9035233.1"
                     /translation="MNFSIRHVNEDDFQLIKLTDETAGTEAVLLPGCGALLHAFRVRG
                     RDGSLFNVIDNYRDSADLRTNIGTSFKGPKLSPFPCRIPDGVYQFDNKNYRLEHLFPD
                     GTAIHGLLYDKPFTVVQEAVGDQGAGLVLEYAYRNDDPGYPFQYNCQVRYTLHNGNDL
                     EIVTTVTNVDKTAIPMADGWHPYFRLGGKVDEWTLQFYSEAIVDFDDRLVPTGHLTPY
                     NTFEKARPVGDLFLDNCFSLKPAIVSAACELYNPSNGLRVCFFPDASYPYLQLYTPSS
                     RESIAVENLSGAPDCFNNGLGLLSLQPGHSQIFTVRYKVSVD"
     gene            2073..2825
                     /locus_tag="VGQ51_01385"
     CDS             2073..2825
                     /locus_tag="VGQ51_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035234.1"
                     /translation="MKLFKWVLSAALAFSFAGCLDIDERLDVNKDGSGQLAMDIDMSQ
                     MVDILQQYMGKEELQKKGLQKMDTTILMKDVIDSSKLSADKKAILRPGRVHIKLDMDA
                     KVFTIHSMFPFKNQADLEKLYTLQNDGSTLGNAHLFGNLGGDSAGGPGDINQFNGIFD
                     FKCRDGLLSKKLNKEKFDALKNDPQMAQIKQATQMGIEINYTSTLSLPRPVKKIDNPL
                     LKLSDDKKTVTMKFNLIDVFDHPGQFEYTVEY"
     gene            2837..3586
                     /locus_tag="VGQ51_01390"
     CDS             2837..3586
                     /locus_tag="VGQ51_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035235.1"
                     /translation="MTRTPSCRTILIVLCCCACHSHPVSPDRTGISEQLIGNITIGTY
                     SGHYSKGLLTMAINYISGNIASGYDIHKGLRRNLNGSVESKDGRLEFVLKEPGGNPYD
                     GTFYLSLDTAADKISGKWVPADSKMAKEGPLELARTTYASNMPDLDNCEWTNDLGTLQ
                     FGEEGVCTLQYYPGEDSVNIADANAQQITVKGSYEVKGDTIRIDWQRNNRTPALNMRL
                     IYHAGSEPTDSTSGAPFLEGKKVKFEKLIAG"
     gene            3589..>4227
                     /locus_tag="VGQ51_01395"
     CDS             3589..>4227
                     /locus_tag="VGQ51_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEV9035236.1"
                     /translation="MRKPLLRIVLLLALAFFSFMMIRLTLPYTAMRSDVAFLRSKQHI
                     YHIRYWRISFYTHVFTSCLVLLAGFTQFAPRLLFRRPRVHRIMGWIYLIGVTTISGPA
                     AFIMALYANGGIAARTSFTLLAIGWISFTLFAGYYAFRRRFVLHGAFMFRSYALTLSA
                     ITLRGYTWIIDLINLPMTPRETYILTAWLAWVPNLLLAELLIRRGWVKKILRK"
BASE COUNT          996 a         1152 c         1070 g         1009 t
ORIGIN      
        1 ggactcatcc actacgggtc agcctggcat ctgatcggct ggtgtcatct gcgcaacgac
       61 taccgggact tccggctcac gcggatgctg ggggtcaacc tggaagaaat taccttcgat
      121 ccctcttctc acccttccat ccgggaatat attgatcggc tcagggccgg tgccgacctt
      181 caggaagtca ttatcctggt cgacaggcgg atcgtcaaat acctgcagga gcagaaatac
      241 ctaaatgggt tcgtttccca ggacgagctg gaagactgca tgcggatgaa gttcctggtt
      301 tcctcattgg attatttcgc acgctggctc ctgatgtata ccgacgccat ccgcgtggaa
      361 actccccgtg agctgcagga ccgtatgcaa aaaatttccg ggcaactgtc cgaacatcac
      421 gggtcctact gacataatgt tgtcattccg gcactgtttc tttgtaccgg tactcaatcc
      481 attaaccaga tcactgaaac acagatcggt ctttttttaa cgtatcaggc aaacacctta
      541 tatatgaaca ccaggacatg tgccgccttc attttcgacg gcttttcaga ccacgagctg
      601 gccattaccg tggccggttt gcaccgcagg gacaatattg tcctcgagac gtttgcgaac
      661 aagggacgca cggccacttc gcgcaccggc ctccggacga tacctcatac cagcctttcc
      721 tatatgtcgc ccgatgattt cgacatcctg gtccttccgg gaggacaaca atgggagaaa
      781 ggcgataatc tcgagatttt tccactgatc acggccacgg caggccgccg tcctatcgtc
      841 gccatcagcg aagccaccct ggcgctggcg gacctgggtc tgctgaacga tatcccgcat
      901 accggtatcc atccggatca tttcgaacgg ttctgccccg attatgaggg agcatcttat
      961 tacaagagtc agccttgtat cgatgcgggt atgatcatca cggtcgacgg gatggcgatt
     1021 acacaatccg gtcatggcat gttgtgcctt ttcgatacat tgcagaacat aattgctcct
     1081 caccatgaac ttttcgatcc ggcatgttaa tgaagacgat ttccaactga tcaaactcac
     1141 cgatgaaacc gccggcaccg aggcagtcct tctccccggc tgcggcgccc ttctccatgc
     1201 tttccgggta cgcggcaggg atgggtcgct attcaatgtc atcgataatt accgcgatag
     1261 cgcggacttg cggacaaaca tcggcacgtc gttcaaaggc cccaagttgt cccctttccc
     1321 ctgccggata cccgatgggg tctaccagtt tgataacaag aactatcgcc tcgaacatct
     1381 ttttcccgat gggacggcga tccatggttt gttgtatgac aagccattta cggtggtgca
     1441 ggaagcggtc ggcgatcagg gcgccgggct ggtgcttgaa tatgcctaca gaaatgacga
     1501 tccgggctat ccgttccaat acaactgcca ggtacgatat acccttcaca acggcaacga
     1561 tctggagatc gtgaccacgg tgacaaatgt cgacaagacc gccatcccga tggccgacgg
     1621 ctggcatcct tatttccggt tgggcgggaa ggtggatgag tggacgctgc agttctattc
     1681 ggaagctatc gtagacttcg atgatcgtct cgtgccaacg ggccacctga ctccttataa
     1741 tacatttgaa aaggccaggc ccgtcggtga tctctttctc gacaattgct tttcgctgaa
     1801 acctgccatc gtcagtgcgg cctgcgagct ctacaatcct tccaacgggt tgcgcgtgtg
     1861 cttctttccc gatgccagct atccctatct gcaactgtat acgccgtctt cgagagaaag
     1921 catcgcggta gagaatctca gcggcgcgcc tgattgcttc aacaatggtc tgggactgct
     1981 ctcgctacag ccggggcatt cacaaatatt tacagttcgc tataaggtta gcgtcgacta
     2041 aaaacctaac ttaccggcaa atcgtgcacg tgatgaagtt attcaaatgg gtactgtccg
     2101 ctgctcttgc tttttctttt gcaggctgtc tcgacattga cgagcggctg gatgttaata
     2161 aagatggtag cggccagctc gccatggaca tcgatatgag ccagatggtc gatatcctgc
     2221 aacaatatat gggaaaagaa gagctccaga agaagggttt gcagaaaatg gacacgacta
     2281 ttctgatgaa ggacgtaata gattcctcta agctcagcgc cgataaaaag gccatcctcc
     2341 gcccgggcag ggtgcatatc aagctggata tggatgccaa ggtttttacg atccatagca
     2401 tgttcccctt taagaaccag gcggatctcg agaagctgta caccctccag aatgacggct
     2461 cgacactggg gaatgcgcat cttttcggca atcttggcgg ggatagcgcc ggcggtcccg
     2521 gcgacatcaa ccagttcaac ggcatcttcg atttcaaatg ccgcgacgga ctgctttcga
     2581 aaaagctcaa taaagagaaa ttcgatgcgc tgaagaacga tccccagatg gcgcagataa
     2641 aacaggccac acaaatgggc atagagatca attatacgag taccctctcg cttcccaggc
     2701 ccgtcaaaaa gatcgacaat cccctgctca agctgtcgga tgacaaaaaa accgtcacga
     2761 tgaagttcaa tctcatcgat gtgttcgacc atccgggaca atttgaatat acggtagaat
     2821 attagtaaat ttctgcatga cgcgaacccc atcctgcaga acgatcctga tcgtactttg
     2881 ttgctgcgcc tgtcattcac acccggtgtc tccagaccgg acaggcatct ccgaacagct
     2941 gatcgggaac attaccatcg gtacttattc cgggcactac agcaagggcc ttctgacaat
     3001 ggccatcaat tacatttccg gcaacatcgc ttccggttac gacatccata agggactccg
     3061 ccggaatttg aatggcagcg tggagagcaa agacggccga ctggaattcg tgctgaagga
     3121 acctggcggt aatccctatg acgggacctt ctatctttcc ctcgatactg ccgctgacaa
     3181 gatctcgggc aaatgggtcc ctgccgatag caaaatggcc aaagagggac cgctcgagct
     3241 ggcgcggacg acctacgctt ccaatatgcc ggatctcgac aattgcgaat ggacaaacga
     3301 tctgggaacg cttcaattcg gtgaggaagg agtgtgcact cttcaatatt atcccggtga
     3361 agattccgta aatatcgcgg acgccaatgc acagcagatc accgtaaagg gcagctacga
     3421 ggtaaaggga gatacgatcc ggatagactg gcaacgcaat aaccgaacgc cggcgctcaa
     3481 tatgaggctc atctatcatg caggcagcga acccaccgat tcgacatcgg gagccccctt
     3541 cctggaaggt aaaaaagtga aattcgaaaa acttattgcg ggctgatcat gcgaaagcct
     3601 ttgttacgga tagtgctgtt gctggccctg gccttctttt ccttcatgat gatccggctt
     3661 acgcttccgt atacggctat gcgaagcgat gtcgcattcc tgcggtccaa acagcatatt
     3721 tatcatatcc gttattggcg cattagtttc tatacgcatg tctttaccag ctgcctggtc
     3781 ctgctcgccg ggtttaccca gttcgcgccc cgcctgcttt tccgccggcc ccgggtccat
     3841 cggatcatgg gctggatata ccttatcggt gtgacgacga tcagcggtcc cgccgccttt
     3901 atcatggccc tgtacgctaa cggcgggatt gcggcacgaa ccagctttac cctgctggcc
     3961 ataggttgga tcagctttac cttatttgcc ggatactatg cttttcgcag gcggtttgtt
     4021 ctgcatgggg cctttatgtt cagaagctat gcgctgaccc tgtcggcgat cacgcttagg
     4081 ggatatacgt ggatcatcga tctgatcaac ctgcctatga ctccgcgcga aacctatatc
     4141 ctcaccgcct ggctcgcctg ggtgccgaat cttttgttgg cagagttgct gattcgccgg
     4201 ggttgggtga aaaagatatt gcggaag
//
LOCUS       DASYSV010000046         6808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_395688, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000046 DASYSV010000000
VERSION     DASYSV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395688"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..2585)
                     /locus_tag="VGQ51_01400"
     CDS             complement(42..2585)
                     /locus_tag="VGQ51_01400"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV9035237.1"
                     /translation="MKRIIYLLAAIWSGGLTVSAQAPSTVTSADIYLRLKKLNVLGTV
                     LYVAAHPDDENSRLIAYMAKDRLYRTGYLSMTRGDGGQNLIGDEQGVELGLIRTQEMM
                     AARRVDGAEQFFTRAFDFGFSKSTEEALKTWDKQKILSDVVWVIRKFQPDVIITRFPP
                     DSRAGHGHHSASAVLAHEAYEAAADPNRFPEQLKYGGIKPWKAKRILWNSFNFGPGAN
                     TTSDDQMKIDVGGYNPILGKSYGELAAISRTNHKSQGAALTPARGESMEYFNLVAGDP
                     AHSDPMEGVVTDWSRVEGAQNIPGMIERVIRDFSLMAPEKSVPGLIDIWNAIAQLPDG
                     YWKNKKMQEVQALIADCSGLWVEATVRTPYAVRGDSLAITCVVNDRLGVDMTVNSISV
                     STPPSVQAEYTVPPGHPDAKLYKPDLSATVDQQLRADQNFTFTRSILVSPRKMLTQPY
                     WLAEPMSPGHFNVKDQQLIGEPLGDPPYLAEVFITVDGHQVEVDRPVRYKYTDPVKGE
                     LYEPLTILPRATARFDPELLVFTDGEKKGFNALVQDRTGLDHSPVLGLTAAPALSIQS
                     SAGGTLGGQSWMAKPAGPETALVTVNGVFDDHGRKDTAMELRTIAYEHIPRIDYFRPA
                     EEKFVLADIKTAGKRIGYIEGAGDKVPDALVQMGYEVMMLKEKDITPGFLNQFDAVIT
                     GVRAYDVHGWLAARHSALMEYVKDGGNLIVQYNRGNLGRMTTNIGPYPLAISNLRVTD
                     ENARVNFLQPSHPVLNFPNKITDRDFEGWIQERGIYFAGQTDPQYEAIFSMSDPGEQE
                     QKGSLVIAHFGKGIFVYTGLVFFRELPAGVPGAYRLIANIIALNQKKGF"
     gene            complement(2595..5129)
                     /locus_tag="VGQ51_01405"
     CDS             complement(2595..5129)
                     /locus_tag="VGQ51_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HEV9035238.1"
                     /translation="MKINLLLPLGLLLSVCCCYSQTAPSPEAFLGYPLGAHFTPHHRV
                     VEYFRAVAAAVPGRVQVEQYGTTYEGRPLVMAFIASPANLLRLESIRRNNLRLAGVLH
                     DSAAPDEHAPVIVWLSYNVHGNEPASTEAAMKTLYALVDPAGEQGKGLLDNTVVIIDP
                     CINPDGRDRYVNWYNDVVGAQPNPDPVSREHQEPWPRGRTNHYNFDLNRDWAWQTQVE
                     TQQRLKKYNSWLPQVHVDYHEQGFNNPYYFAPAAEPYHDVITPWQREFQVTIGKNNAR
                     FFDRKGWLYFTKEEFDLFYPSYGDTYPIYNGAIGMTFEQGGIGAGLAVQLSDGDTLTL
                     AQRIEHHYTTGISTIGMAAANADRLVREFHHYFIEAVAHPGGEFKAYILRNDTFGDRL
                     ERLRGFLDRNGIKWDLVAAGTYTGVDYETGKSATFKTAPGDIVINANQPKSNLLRVLF
                     ERQSHISDSVTYDITAWSAPFAYGIQAYGLNAFAGVEKPHIGGTGSTGVDEQYAYAIR
                     WTGMGSVRLLSRLLQKGIKIRFAERPFQSGGQSFDRGTLLITATGNSALGAGLWPLVK
                     GTAKETGVNLWPIASGFVEKGPDLGSGLVRFIRRPRVALLTGEQVGSQEAGEVWHLFE
                     QELGYPITLVNAESMAYVAWKNFDVVILPGGRYRGLEDKNTAEMLRNWVQEGGRLIAM
                     EQAVEQLAKGEWGIRAKAADGDKKEDEKDKKDDEYADLHRYDNRQREEVTGSVPGSIY
                     KVELDNSHPLAFGYPDHYYTLKQDDNVYEFMKDGGWNVGVVRKDGYVSGFTGTRAREK
                     IRDGVIFGVQDLGRGEVVFMADDPLFRSFWENGKMLFCNAVFLVGQ"
     gene            5181..>6808
                     /gene="ggt"
                     /locus_tag="VGQ51_01410"
     CDS             5181..>6808
                     /gene="ggt"
                     /locus_tag="VGQ51_01410"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEV9035239.1"
                     /translation="MRQPLIRFIIHCTLLLVSVSALAQNDQTPFSYTIRKTAFVNHGA
                     VVSAHPLASHAGMEILRQGGNAFDAAITTQLVLAVVYPAAGNIGGGGFMVAHTADGKN
                     ITLDYREKAPTAATRDMYLDADGNPRTDLSQNGHLASGVPGTIAGIFDELRYAKLGFP
                     RLIQPAIDLAENGFALTASEARGLNSAQKAFRKYNTVLPVFVNERGWKAGDTLIQKDL
                     ARTLRRIRDLGAKGFYEGETARLIVAEMQRGKGIITMSDLKGYTAKERQPAIFPYKQY
                     TVVTMPLPSSGGVILQQMMKMVEDQPLASYGFGSVKAVHLMTEVERLAYADRAKYLGD
                     RDFYSVPVDTLTSHAYLQRRMAEIQPARAGSSKDIGAGMLSPKSEETTHFEVVDDQGN
                     AVSITTTLNGGYGSKVVVGGAGFFLNNEMDDFSVKPGVPNMYGAVGTEANAIAPGKRM
                     LSSMTPTIVLEKGKVYLVVGTPGGTTITTSVFQTLLDILDFGLSAEDAVNKPKFHHQW
                     LPDSLYVEKTFPSDTREKLTNMGYNISQRGSIGRT"
BASE COUNT         1532 a         2179 c         1654 g         1443 t
ORIGIN      
        1 gaccagagcc gtaatgacgg caacatccct ccttctcctc atcagaaacc cttcttttga
       61 ttaagtgcaa taatattcgc tatcaggcgg taagcccccg gcacgccggc gggcaattcc
      121 ctgaaaaaca ccagccccgt ataaacgaaa atccctttcc cgaaatgcgc gatcaccagg
      181 ctgccttttt gttcctgttc tcccggatcg ctcatcgaaa atatcgcctc atactgagga
      241 tcggtctgcc cggcaaaata aatgccgcgc tcctgtatcc agccctcgaa atccctgtcg
      301 gtgatcttat tgggaaagtt gagcacggga tgcgacggtt gcaggaaatt gacccgcgca
      361 ttctcatcgg tcacccggag attcgatatc gcaaggggat acggtccgat attggtggtc
      421 atccgaccca ggttgcccct gttgtactga acgatcaggt tcccgccgtc tttgacatat
      481 tccatcagtg cgctatgccg ggccgccagc caaccatgta cgtcataagc ccggaccccg
      541 gttatgacgg catcgaattg attcaaaaag cccggggtaa tatccttttc tttcagcatc
      601 atcacctcat aacccatttg caccaacgca tccgggactt tgtccccggc tccttcgata
      661 taaccgatgc gcttcccggc cgtttttatg tcggccagca caaacttctc ttccgccgga
      721 cggaaatagt cgatccgcgg gatatgttcg taggcgatcg ttcggagctc catcgcggtg
      781 tccttccggc catggtcatc gaaaacgcca ttcacggtga ccagcgccgt ctccgggccg
      841 gcgggtttcg ccatccagga ctgtccaccc aatgtcccgc ccgcggaaga ttgtatggaa
      901 agcgcaggtg cggccgtcag tcccaaaacg ggtgaatgat caagccccgt acggtcctgc
      961 accaacgcgt tgaatccctt tttctcgccg tcggtaaata ccagtaactc cggatcgaac
     1021 cgcgccgtag cccgcggcag tatcgtcagg ggctcgtaga gctcgccttt caccggatcg
     1081 gtatacttgt atcgaaccgg tcgatccacc tccacctgat gcccgtctac cgtaatgaag
     1141 acctccgcca ggtaaggcgg atcacccagc ggctcgccga tcagttgctg gtcctttaca
     1201 ttaaaatggc cgggtgacat cggctctgcc agccaatagg gctgtgtcag catctttcgc
     1261 ggcgacacga ggatcgatcg cgtaaaggtg aaattctggt ccgcacgcag ctgctgatcc
     1321 acagtcgcag aaagatccgg cttataaagc ttggcgtcgg gatgccccgg tggaacagta
     1381 tattccgcct gcaccgacgg aggcgtcgac acagagatcg aattgacggt catatccacc
     1441 cccagccggt cattcaccac gcaagtgatg gcaagcgaat cccctctcac cgcataaggt
     1501 gtccgcacgg tcgcctccac ccataatccg ctgcagtcgg cgatcagcgc ctgcacctcc
     1561 tgcatcttct tattcttcca atatccatcg ggtaactgag cgatagcatt ccagatatct
     1621 atcagacccg gcacactttt ctcgggagcc atcagcgaaa aatcccggat cacccgttcg
     1681 atcatccccg ggatattctg tgctccttct accctgctcc aatccgtgac cacaccctcc
     1741 atcggatcgc tatgcgccgg atcaccagcc acgaggttaa agtattccat cgactcacca
     1801 cgggcaggcg tcagcgctgc accctggctc ttgtgattgg tcctgctgat cgcggccagc
     1861 tcgccatagc ttttccccag tatagggttg tagccaccga catctatctt catctggtcg
     1921 tcgctcgtcg tattggcgcc gggaccgaaa ttgaaggaat tccataatat ccgcttcgct
     1981 ttccagggct ttattccgcc atatttcaat tgctccggga aacggttcgg atcggccgcc
     2041 gcctcatatg cttcatgtgc aagcaccgca ctggccgaat gatggccatg acccgcccgg
     2101 ctgtccgggg gaaatcgcgt gatgatcaca tccggctgga actttcgtat cacccaaacg
     2161 acatcgctca atatcttttg cttgtcccag gtctttaacg cttcctccgt cgatttcgaa
     2221 aagccgaaat cgaacgcacg ggtaaagaat tgctcggccc catccacccg ccgcgccgcc
     2281 atcatctcct gcgtacggat cagccccagc tcgacgccct gctcgtcgcc aatgagattc
     2341 tgaccgccat cccccctggt catcgaaaga tatcccgtcc ggtatagccg gtccttggcc
     2401 atatacgcga tcaaccgcga attctcatcg tcgggatgcg ccgccacata gaggacggtg
     2461 cccaggacat tcaatttttt tagccgtaaa tagatatccg ccgaggtcac ggtggaaggc
     2521 gcctgcgccg agaccgtaag accccccgac cagatcgccg ccaataaata aatgattcgt
     2581 ttcatagtaa tatattattg cccgactaag aacaccgcat tgcaaaacaa cattttccca
     2641 ttctcccaaa aactgcggaa taaaggatca tccgccatga atacgacctc accccttccc
     2701 aggtcctgca caccgaagat caccccatcc cttatctttt ccctggccct ggtacccgtg
     2761 aacccgctca catatccgtc cttccttacg acaccgacat tccagccgcc gtccttcatg
     2821 aattcatata cattatcgtc ctgcttcagc gtataataat ggtcgggata cccgaatgcc
     2881 aacggatgag aattgtccag ctccacttta tagatcgatc cgggaacgct gcccgtgacc
     2941 tcctcgcgct gccggttgtc ataccggtgc agatccgcat attcgtcgtc ctttttgtcc
     3001 ttttcgtctt ccttcttatc cccgtccgca gcctttgccc tgatccccca ttcaccctta
     3061 gccagctgct ccacggcctg ttccatggcg ataagacgcc ctccctcctg aacccagttg
     3121 cgcaacattt ctgcggtatt cttgtcctcc agcccccggt acctgccgcc gggaaggatc
     3181 acgacgtcga agttcttcca ggctacatat gccatagatt ccgcatttac caacgtgatc
     3241 gggtaaccga gctcctgctc gaacagatgc cagacctctc ccgcctcctg cgagcccacc
     3301 tgctcgcccg ttaacagcgc cacccgcgga cgacggataa aacgtaccag accgctgcca
     3361 agatcggggc ccttttcgac gaacccggag gcaatgggcc ataggtttac accagtctcc
     3421 ttcgccgtcc cctttaccag gggccataat ccggcgccca gcgcgctatt gccggtggcc
     3481 gtgatcagca gggtgccgcg atcgaaggat tggccccccg attggaaggg ccgttccgca
     3541 aagcgtatct tgatgccttt ctgcaacagc ctgctcagca aacgcacgga gcccatgccc
     3601 gtccatcgta tggcataagc atattgttcg tccactccgg tcgaccccgt accaccgata
     3661 tgcggtttct ccactccggc aaaagcattc aacccataag cctgtatccc ataagcgaaa
     3721 ggcgcagacc aggccgtaat atcataagtc accgagtcac tgatatgcga ttgccgctcg
     3781 aatagcaccc gcaacaaatt cgatttcggc tgattggcat tgatcacgat atcgcccggc
     3841 gcagtcttga aagtcgcact cttacccgtc tcataatcca ctcccgtata ggtgccggcc
     3901 gccacaagat cccatttgat gccgttccgg tcgaggaaac cccgcagccg ctcaagcctg
     3961 tccccgaagg tgtcgttccg aaggatatat gccttgaact cgccgccggg atgcgcgacc
     4021 gcttcgataa aataatgatg gaattcccgc accagccggt ccgcattggc cgccgccatc
     4081 ccgatggtcg atatccccgt cgtataatga tgctcgatac gctgcgcaag cgtcaatgtg
     4141 tccccgtcgg ataactgcac cgccagccct gcgccgatgc cgccctgctc gaaggtcatg
     4201 ccgatggcgc cattatagat cgggtaggta tccccgtaac tgggatagaa caggtcgaac
     4261 tcttctttcg taaaatacag ccagcccttg cgatcgaaaa aacgcgcgtt attttttccg
     4321 atggtgacct ggaattcgcg ctgccacgga gtgatcacgt catgataagg ttccgcggcc
     4381 ggagcgaaat aatacggatt gttgaaaccc tgttcatggt aatccacatg cacctgcggt
     4441 aaccaggaat tgtatttctt caaccgctgc tgcgtttcca cctgcgtctg ccaggcccag
     4501 tcgcggttga gatcgaaatt gtaatgattg gtccggcctc tgggccaggg ctcctggtgc
     4561 tcgcgcgaca ccgggtcggg attgggctgc gcacccacga catcattgta ccagttgaca
     4621 tacctgtcgc gaccgtcggg attgatgcaa gggtcgatga tgacgaccgt attatccaac
     4681 aatcccttgc cctgctcacc cgcaggatcc acaagagcat acagggtctt catggccgcc
     4741 tcggtggatg ccggctcgtt gccatgcaca ttatagctca accaaacgat cacaggcgca
     4801 tgttcgtccg gcgccgcact gtcatgaagc acccccgcga gccgaagatt attccgccgg
     4861 atggactcca ggcggagaag atttgcggga gacgcgataa aggccatcac cagcggccgt
     4921 ccctcatacg tggtcccata ttgttccacc tgtaccctgc ccggcacagc cgccgccacc
     4981 gccctgaaat attcgacgac acgatgatgc ggagtgaaat gcgcgcccaa tggatagccc
     5041 aaaaatgctt ccggcgaagg cgccgtctgt gaataacaac agcagacaga cagcagcaga
     5101 cccaatggca atagcaggtt tatcttcatg aatgggcgaa tgtaagaaaa atggtaactt
     5161 acggtaaatc tttcttctgt atgcggcaac cgctcatccg ctttatcatc cattgcacac
     5221 ttctcctcgt ttcggtcagc gctcttgccc aaaacgacca gacacccttc tcctatacca
     5281 tccgcaagac ggccttcgtg aatcacggcg ccgtggtctc cgcacatccc ctcgccagcc
     5341 atgcaggcat ggagatcctg cgtcagggtg gcaacgcctt cgatgcagcg atcacgacgc
     5401 agctggtact ggcagtcgta tatcccgccg caggtaacat cggcggagga ggcttcatgg
     5461 tcgcacatac ggccgacgga aaaaatatca cgctcgacta ccgtgaaaaa gcacccactg
     5521 ccgccacccg cgacatgtat ctcgacgccg atggcaaccc tcggacggat ctcagccaga
     5581 acgggcatct cgcgtccggc gtaccgggca ccatcgccgg catatttgac gagctgcgtt
     5641 atgcaaagct gggcttcccc cgtttgatac agccggccat cgatctggcc gaaaacggct
     5701 ttgccctcac agcatccgag gcacgcggtc tcaactccgc ccagaaggca tttagaaaat
     5761 acaatacggt cctccccgtt ttcgtaaacg aaagaggatg gaaggctggc gatacactga
     5821 tacaaaaaga cctcgcccgc accctgagac gcatccgcga cctgggcgcc aaaggcttct
     5881 atgagggcga aacggcccgg ctcatcgtcg cggaaatgca acgcgggaaa ggtatcatca
     5941 cgatgtccga tctgaagggt tataccgcca aagaaagaca accagccatc ttcccctaca
     6001 aacaatacac ggtcgtaacc atgccgctgc ccagcagcgg cggcgtcata cttcaacaaa
     6061 tgatgaaaat ggtggaagat caacccctcg cctcctacgg tttcgggtcg gtgaaagccg
     6121 tccacctcat gacggaggta gaacgcctcg cctatgccga cagggccaaa tatctcggcg
     6181 accgcgactt ctattcggtg cccgtcgata ccctcaccag ccacgcatac ctgcaacgaa
     6241 gaatggccga gatccaacca gccagggcgg gcagcagtaa agatatcggc gcaggcatgc
     6301 tttctccaaa gtccgaggag acgacccatt tcgaggtcgt cgacgaccag ggcaacgccg
     6361 tgtccatcac gaccacgctc aacggcggat atggcagcaa ggtggtggtc ggcggcgccg
     6421 gcttcttcct caacaacgag atggatgact tcagcgtcaa acccggagta cccaatatgt
     6481 acggcgccgt tggcacggaa gccaatgcca tcgcacccgg caaacggatg ctaagctcta
     6541 tgactcccac gatcgtcctc gaaaagggaa aagtgtatct cgtagtagga acgccgggag
     6601 gtaccaccat caccacttct gtcttccaga ccctcctcga catcctcgat ttcggtctca
     6661 gcgcggaaga cgccgtcaat aaacccaaat tccaccacca gtggctgccg gactcgctct
     6721 acgtggaaaa gacgtttcct tccgataccc gcgaaaaatt gacgaacatg ggctacaata
     6781 tcagccagcg cggcagcatc ggccgcac
//
LOCUS       DASYSV010000047        14941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1292873, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000047 DASYSV010000000
VERSION     DASYSV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 14941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14941
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292873"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="VGQ51_01415"
     CDS             <1..1339
                     /locus_tag="VGQ51_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9035240.1"
                     /translation="QSPATVRFTQRVIDYWLTNYRIDGFRWDLAKGFTQTRTCDVQGN
                     NCDVAAWGHYDAGRVATWDTIYNQMQTASPGSYCILEMFADNSEQQVEANYGMLLWGE
                     DLSANYNQATMGYNTPSPGGATWDLSGSIYTSLGGWNAPGLVVYQDSHDDERLQYNNE
                     QYGNHGGSYNITDTATGLARDAMATAFWALAPGPKMLTEFSELGFDYSINWCTNGTVD
                     QSGSCRLVPKPIRWDYLRDSARKKLHDVHSSLLRLLANYPGLRTGATNYSLAGNVKYL
                     GVSSDSLSVMVIGNFDVVPSTASISFPSAGIWYDYLSGKTLQATGGSQQMMFAAGEYH
                     VYTNKNLGGPDTTTTSPVPVSPAGLAIYPNPAGIGSATITYRLPVAGNSVLEVYSISG
                     ARIATLYLGSQGAGTYSLPAGRLYDPASLPNGLYVLKLVGAGITAHFPFLVLH"
     gene            1393..1812
                     /locus_tag="VGQ51_01420"
     CDS             1393..1812
                     /locus_tag="VGQ51_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035241.1"
                     /translation="MRNTFLLFVLVSFTLLAACHKNEHPVCCEPFYYFADSGSWRLVQ
                     VSGGIGGRTTTIPADSLVLLALHGDWRYNNSVNGHTTDTGRFGIAEVATGTLTDTIGL
                     KFSGDTSILYLCRASLDRMVLTQFVSDGFQFTYLKNK"
     gene            complement(1819..2427)
                     /locus_tag="VGQ51_01425"
     CDS             complement(1819..2427)
                     /locus_tag="VGQ51_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEV9035242.1"
                     /translation="MSTPILSLGRSTFGDPANKDRVLSREQAYTLLEDWVPNERLRLH
                     MKQVGAVMRAWAMEREGADERTAFKWELAGLLHDADWEKHPDAHCRIIIEHLESLKID
                     PEVIHCIASHGPKHFGVEPETTMDKMIYVFDELSGFIHAAALIRPTRYEGMDVKSITK
                     KLKTPSFAAQVSRDDINDALSRVNIPLEEIIQFIIDHQKDVQ"
     gene            2674..3117
                     /gene="rplM"
                     /locus_tag="VGQ51_01430"
     CDS             2674..3117
                     /gene="rplM"
                     /locus_tag="VGQ51_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV9035243.1"
                     /translation="MSKLHFTTKHANEATVKRDWYVVDGTNQTVGRMCSKIAAVLRGK
                     NKAYYTPHVDCGDFIIVINADKVVFTGNKAEDKIYINFSGYPGGKKEEIAKNLMRRRP
                     DAVIERAVKGMLPKNRLGRKMFKKLFVYAGAEHPHTAQQPKTLTF"
     gene            3130..3519
                     /gene="rpsI"
                     /locus_tag="VGQ51_01435"
     CDS             3130..3519
                     /gene="rpsI"
                     /locus_tag="VGQ51_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV9035244.1"
                     /translation="MEKQKNAVGRRKEAVTRVFLSRGDGKITINDKDYKQYFSLVYLQ
                     NQVELPLKTVEGIDKYDVKINATGGGMKGQAEAAKLGIARALLEVNAEFRPALKAAGL
                     LRRDPRSVERKKPGRKKARRSYQFSKR"
     gene            3553..4425
                     /gene="rpsB"
                     /locus_tag="VGQ51_01440"
     CDS             3553..4425
                     /gene="rpsB"
                     /locus_tag="VGQ51_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV9035245.1"
                     /translation="MENNTSLQQQLLEAGVHFGHLRKKWNPKMLPYIFAEKKGIHIID
                     LNKTTESLQEAAAALKQIAKSGKKVMFVGTKKQAKEIVTECARKVNMPFVTERWLGGM
                     LTNFNTVRKSVKQMQSIEKMLNDGTFDSITKKERLTLSREKDKKEKVLGGIAQLGRVP
                     AALFIVDIGHEHIALAEAKRLGITTFGMVDTNCDPNKVDFAIPANDDATKSIAIIVNY
                     VTAAIAEGLAERQAAKEEDVEDSGSDENERKAARLQAEAESEASRGRGEKVKGTSQPK
                     RRIPNSGPRRPGGR"
     gene            4452..5300
                     /gene="tsf"
                     /locus_tag="VGQ51_01445"
     CDS             4452..5300
                     /gene="tsf"
                     /locus_tag="VGQ51_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEV9035246.1"
                     /translation="MSTATITAADINKLRQTTGAGMMDCRKALVESDGDFEKAIDYLR
                     KKGQKVAALRSDREAKEGVVIATTTPDNKTGYIITVACETDFVAKNADFVAFAQSIVN
                     VALKENIKTLEELQSARLDGVTISDRINDQVARIGEKIQLARFERIDAEGVAAYIHGA
                     YRMSVLVGLSKNTPAVLEAGKDIAMQIAAMNPVAVDPDSVSADVVAREKAIVTEQIRN
                     DPKMAGKPDEMIDKIAVGKLNAFFKENTLTAQAFVKDASKTVGDYLKGVDPTVKVTQF
                     KRVQLG"
     gene            5344..6057
                     /gene="pyrH"
                     /locus_tag="VGQ51_01450"
     CDS             5344..6057
                     /gene="pyrH"
                     /locus_tag="VGQ51_01450"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV9035247.1"
                     /translation="MIPKYKRVLLKLSGEALMGDKSFGFDNAVIAQYAQDIKRIVELG
                     VQVAIVIGGGNIYRGMNEAETGIERAHGDYMGMLATVINGMALQAGLEKIGVYTRLQS
                     AIKMEQIAEPYIRRRAIRHVEKGRVVIFGAGTGNPYFTTDTAGSLRAIEINAEVILKG
                     TRVDGIYTADPEKVPTAEKFESITFQECISRNLRVMDMTAFTLCMENQLPIIVFDMNK
                     PGNLLRVVRGEKVGTLVKD"
     gene            6293..7402
                     /locus_tag="VGQ51_01455"
     CDS             6293..7402
                     /locus_tag="VGQ51_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035248.1"
                     /translation="MNTLNNLNIILGMELTMSLPVIIICELGALILGFTIHFVWNSKK
                     SLPINDPSPSAGISEDDNWKLKYYNDMDMQERSQQQLRDRLSKAQETEQVLNIELDES
                     RQELDSLRLELDEVRAQLEETEARLVPPATEENGVPADQQPEPDYAGNYLYQLKAAQE
                     KLIEHNGHIQRLLEQTQLVEESEKKNRELTQLNEDINARLREAGQLLIEKETEISNLR
                     HQQKLSEEMTQRLDKVYEEYNTLQDKLQKLQAYLTQPYNRGTDYEGLKESYFKLGKEH
                     DEMKLKYISIREENQRITRILGDTEEKLKEANFQRLQYQKRSAFLEELNHDLQEIAEH
                     NKKIESQLRRVTDMENLLARITGSGTINDIAQGME"
     gene            7441..8082
                     /gene="pdxH"
                     /locus_tag="VGQ51_01460"
     CDS             7441..8082
                     /gene="pdxH"
                     /locus_tag="VGQ51_01460"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEV9035249.1"
                     /translation="MSTIADIRKEYKLQTLSEDDILKDPFAQFGKWWQEAMNAGIEEP
                     NAMTLATATADGMPDARIVLLKGFDERGFTFYTNYNSTKGMQLLENPRGALVFFWKEL
                     ERQVRIGGLINLAPARISDEYFDSRPEGSRIGAWASPQSEPISSREWLEDNERRYREL
                     WQGKMIVRPPHWGGYVLKPVRIEFWQGRPSRLHDRFLFTLQSSGPWKIERLAP"
     gene            complement(8118..8234)
                     /locus_tag="VGQ51_01465"
     CDS             complement(8118..8234)
                     /locus_tag="VGQ51_01465"
                     /inference="COORDINATES: protein motif:HMM:NF028380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HEV9035250.1"
                     /translation="MGRGDKKTKKGKIFRGSFGKSRPAKRTKKSATSAAKGK"
     gene            complement(8361..8630)
                     /locus_tag="VGQ51_01470"
     CDS             complement(8361..8630)
                     /locus_tag="VGQ51_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV9035251.1"
                     /translation="MNKAELIAKIADDAGITKTQANASLDSFVEAVTKTLKGNGKVTL
                     VGFGTFSVSKRAARNGRNPQTGAVIKIKAKKVAKFKAGKELSAKL"
     gene            complement(8744..9049)
                     /locus_tag="VGQ51_01475"
     CDS             complement(8744..9049)
                     /locus_tag="VGQ51_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HEV9035252.1"
                     /translation="MIVYNITVKIRWDIIEGWLTWLKEEQIPAVLSTGTFDSYQLYRL
                     LEQEDEEGPTFVTQYFTTSLERCEQFVTGFAPRFQEEARKKWGDGFIAFRTLMESVG"
     gene            complement(9036..9947)
                     /locus_tag="VGQ51_01480"
     CDS             complement(9036..9947)
                     /locus_tag="VGQ51_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035253.1"
                     /translation="MKKRRWSDRWLPLGLVLLSLHGAAQDSFHYRAPLDTIKSDAFYW
                     VLLPPSLVAKSRPDLGDLRILGPDNRFVPYVLKDIPRALLNIPEVAPEQKDSSDKHSY
                     ITLRFTEAYEIDRVVLVINSPQYYKRSAYITAESPVPGQWEPVTRIMIDPAHILFHIP
                     STKTHCLHIDIANEDNPPLVISRIACSQICRYMVSYLTAGEGYFVLTGNPAAAVPDYD
                     LKYFTDSLIGEMHYLTPGPLQYLTGKGQPASQAAARTVNKKTAAAERQSGVLLWVILS
                     TVLIFLIYFSVKMVGAIGTKKSPHDRI"
     gene            complement(9925..12264)
                     /locus_tag="VGQ51_01485"
     CDS             complement(9925..12264)
                     /locus_tag="VGQ51_01485"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEV9035254.1"
                     /translation="MEEILIILVILLIVLVMVMRTTLLGRFDALEHEIRRLREEKPAD
                     TPVARPAPPEPHRPQTPAEPDRYPGLAEPHRYPVPPPQETEPFRPIPEARPQTPAVRT
                     PEPAQEPEPGFFERHPDLEKFIGENLVSKIGIAILVLAIGFFVKYAIDNDWIGPAGRV
                     GVGILCGGILIGIAHWLRHRYKAFSSVLVGGGLAVLYCTIALAYHQYQLFGQTAAFLI
                     MLVITAFSVILSLLYDRQELAIIALAGGFITPFLVSNGRGDYVVLFSYLLILNTGLLV
                     IAYHKTWRLLNILAFGFTALLFFSWLVSLPYDASSAVYRGGWSFATTFYLLFFAINIA
                     HTIRKTRPFIASDFIILLSNTCLYFSASLYFIEQLGLYRYKGLFCAVMGVFHLVATWF
                     LFRQRTVDKNILYLLVGITLSFVSLTAPLQLHGHYITLFWASESVLLSWLFVRSGINL
                     IRLSALLVWIAMLISLLMDWFNIYGPVIYDMPAANGPGALPIVFNKGFMTGLYSALCC
                     GAIYYLRKRTPIARIALIAGLVLLFADGALEIAHQFAFYFHGIDLAPLYLLLYTYLFI
                     TLLTAVTRGKHDFRLMNAVLQYAAIFLYLVSLIYIFGLQETMLYKNRYGVHFIAHWTG
                     DVLIVWIMWQLVVAARARTLSMDPALFTWLICIITVILLSAEGQLLVNSLFYSKQNDL
                     PELGRVYDKTVLPILWGLCSFTFMWMGMRHKFQPLRIVSLVLFSITLLKLFLFDIRNI
                     PVAGKIAAFFCLGVLLLVVSFMYQRLKKIIIEDEEKKMV"
     gene            complement(12294..13073)
                     /locus_tag="VGQ51_01490"
     CDS             complement(12294..13073)
                     /locus_tag="VGQ51_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV9035255.1"
                     /translation="MSSLRIVSYNVNGIRAAMNKGLIEWLKTDVADIVAIQETKAHRD
                     NVAHALFTELGFHDYWFPAKKKGYSGVAVFTKVRPDHVEYGTGHTVSDDEGRVIQLDF
                     GDIRLINAYFPSGTSGEERQGYKYRWLDEFFGYLGTLKEKYPRLILCGDYNIAHREID
                     IHDPKGNKNNSGFLPDERAWMDKFITSGWIDSFRHLNPEPHRYTWWSQRFPGVREQNK
                     GWRIDYISVTDPLKGQLLKAEIYPDVRQSDHCPVFLELSVE"
     gene            13107..13865
                     /locus_tag="VGQ51_01495"
     CDS             13107..13865
                     /locus_tag="VGQ51_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV9035256.1"
                     /translation="MDHTDLIIPSRETGYIDGPRSRTYELRFAWGIFRELIRGFRTLH
                     FVGPCITVFGSARFKEDHPHYQSAREIGRQIAGLGYTTMTGGGPGIMEAANRGAFEAG
                     GMSVGCNIKLPFEQKPNPYVQTSITFDHFFIRKVMLVKYSYAFVIMPGGFGTMDEFFG
                     TLTLVQTKSLLQFPIVLFGAEYYKPLFAYMEDMAREGTVSKEDLSLVLLTDSIAEAIR
                     HIDTFIKKNYPQKPRRRKWWLFEPRPALPLAGKA"
     gene            complement(13901..14665)
                     /locus_tag="VGQ51_01500"
     CDS             complement(13901..14665)
                     /locus_tag="VGQ51_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035257.1"
                     /translation="MSFAQQQPPKKKKNKLIKEVYVSWGYNKEWYTHSSLHVSQSALG
                     NDYTLEHVKAHDHPGWDEAILTKQLSIPQYNYRLGFLLDEKRGWGFEINFDHTKYIIA
                     DQDVHIKGKLENHPVDSSIAFNSANGFYYYLNNGANFLLFNVTKRYHVLTTRHEKLKL
                     DLYGKLGVGPVIPHTQNSLFGNPNHQGFQFGGWNTGFEGVIRATFFRYLYLEYSNKFD
                     YARYSGVNVYLGKARQAFGTYEMILSLGIDIPTSRK"
     gene            complement(14794..>14941)
                     /locus_tag="VGQ51_01505"
     CDS             complement(14794..>14941)
                     /locus_tag="VGQ51_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEV9035258.1"
                     /translation="GWYLAHPESKYFGVGKIEKDQVIDYAARKGMPVEEAERWLRPVL
                     EYDV"
BASE COUNT         3794 a         3889 c         3919 g         3339 t
ORIGIN      
        1 ccagagtccc gccacggtcc ggttcacgca gcgtgtgatc gactactggc tcaccaacta
       61 tcgcatcgac gggttccgct gggaccttgc caagggattt acccagacaa ggacatgcga
      121 cgtccagggc aataactgcg atgtcgccgc ctggggccat tatgacgccg gacgcgtcgc
      181 cacctgggat acgatctaca atcaaatgca gacggcctca cccggcagct attgtatcct
      241 cgaaatgttt gccgataaca gcgagcaaca ggtggaagcc aactatggaa tgttgctctg
      301 gggagaagac cttagcgcca actacaatca ggcgaccatg ggttacaaca ctccttcacc
      361 cggcggtgcc acctgggatc ttagcggtag catctatacc tcgctcggcg gctggaatgc
      421 tcccgggctt gtcgtttacc aggacagcca tgacgacgag cggcttcaat acaacaacga
      481 acaatatggc aatcacggcg gcagctataa tattacggac acggctaccg gtctggctcg
      541 cgatgccatg gcgaccgctt tctgggctct ggcgcccggg cccaagatgc ttacggaatt
      601 cagcgagctg ggcttcgact attccatcaa ctggtgtacc aatggcaccg tcgatcagtc
      661 gggctcctgc cgcctggttc ccaagccgat ccgttgggac tatctccggg atagcgcgag
      721 aaagaagctg cacgatgtgc attcctcttt gctgcggctc cttgccaatt accccgggct
      781 caggacaggt gccaccaact attcgctggc gggcaacgtc aaatatctcg gtgtcagttc
      841 agattcgctg agcgtcatgg tcatcggaaa tttcgacgtc gtcccctcaa cggccagcat
      901 atccttcccc tccgccggca tatggtacga ttatctctcc ggaaagaccc tccaggcgac
      961 cggcggttcg cagcagatga tgtttgccgc cggagaatat catgtttata caaataaaaa
     1021 cctcggcggc cccgacacta cgaccaccag ccctgttccc gtgtccccgg cgggactggc
     1081 tatctacccc aaccccgcag gtatcggcag cgctacgatc acctatcgtt tgcccgtcgc
     1141 cggtaacagc gtattggaag tctattctat cagcggcgcc cgcatagcga ccctttatct
     1201 cggcagccag ggcgccggga catattccct gccggcagga cgcctgtatg acccggcatc
     1261 tttgcccaac gggctttatg tgttgaagct cgtaggcgcc ggcatcaccg cgcattttcc
     1321 tttccttgtc ctccattagg cggggagcgt gcaacgatgg ccgaatattg attgtcatat
     1381 gaaaaaaatc ctgtgcggaa tacctttcta ctgtttgtat tagtatcgtt caccctttta
     1441 gcagcctgtc ataagaacga gcatcccgtc tgctgtgagc ctttctatta cttcgcggat
     1501 agcggtagct ggcgcctcgt acaggtctcc ggtggaatag gagggaggac gacaacaatc
     1561 ccggcggact cgcttgtcct gttggccctc catggggact ggcgttacaa caatagcgtc
     1621 aacggccata cgacggatac gggtcggttt gggatcgccg aagttgccac tggtacgttg
     1681 acggatacta tcgggctgaa gttctctggt gataccagca ttctgtacct gtgccgggcc
     1741 agcctggata ggatggtgct gacccaattc gtaagcgacg gttttcagtt tacttacctg
     1801 aagaataaat gatcgggctt attgcacatc cttttggtga tcgatgatga actggatgat
     1861 ctcctccagc ggaatattga cgcggctaag ggcatcgttg atatcgtcgc gactgacctg
     1921 cgcggcaaaa gagggagtct ttagtttttt cgtaatgctt tttacgtcca tgccttcata
     1981 acgggtaggg cggatcaatg cggcggcatg gataaacccc gacagctcgt cgaacacata
     2041 gatcatcttg tccatcgttg tttccggctc tacaccgaaa tgtttgggtc catgcgatgc
     2101 aatgcagtgg atcacctccg gatcgatctt cagtgattcc aggtgttcga tgatgatgcg
     2161 gcaatgtgcg tcgggatgct tttcccaatc ggcatcatgg agcagacccg ccagttccca
     2221 tttgaaagcc gttcgctcat cggcgccctc gcgttccatc gcccaggcac gcatgacggc
     2281 ccccacctgt ttcatatgca gccgcagccg ctcgttcggt acccaatcct ccagcagggt
     2341 ataggcctgc tcgcggctaa ggacacggtc tttattggcc gggtcgccga atgtggagcg
     2401 cccgagggag aggatggggg tggacattgt tcaggatttt ttggatgaag ttatcggttt
     2461 gggtcgacat ttttgcagca gggcaaaatt tttattgtaa atcaattaat tacaaatgcc
     2521 cccttaaaaa taccccttca tttctttgga acatcactct aaataaatct acctttgcac
     2581 tcctttcgaa agccgcctca tatgcggccc gggaagggca aaccaaagaa gtgccgtcgg
     2641 gatagcgacg gcttcattca aaaacacaag aaaatgagca aattacattt cacaaccaaa
     2701 catgcgaacg aggctaccgt taaacgcgac tggtatgttg tggacggtac taatcaaacc
     2761 gtaggccgca tgtgttccaa gatcgccgca gtgcttcgtg gcaagaacaa ggcctattac
     2821 accccgcatg tggattgcgg tgatttcatc atcgtcatca atgcggacaa ggtcgtcttc
     2881 acgggtaaca aggcggagga caagatctac atcaatttct cgggttatcc cggcggcaaa
     2941 aaagaagaga tcgccaagaa tctcatgagg cgccgtcccg acgccgtcat cgaaagggcc
     3001 gtcaagggca tgctgccgaa gaaccggctg gggcgcaaga tgttcaaaaa acttttcgtc
     3061 tatgccggtg cagaacatcc gcacaccgca cagcaaccca aaactttaac cttctaattt
     3121 ttctgataaa tggaaaagca aaaaaatgca gttggtcgcc gtaaagaggc tgtaacccgg
     3181 gtgttcctca gcaggggaga cggcaagatc acgataaatg acaaagacta caaacaatat
     3241 ttctcgctgg tatacctcca gaaccaggtc gagcttccgc taaagacggt cgaaggcatc
     3301 gacaagtacg atgtgaagat caatgcgacc ggcggaggca tgaaaggaca ggccgaagcc
     3361 gcaaagttgg gaatcgcccg cgcgctgctg gaagtgaacg ccgaattccg ccccgcgttg
     3421 aaggcggcag gtcttctgcg tcgcgatccc cgctccgtgg aaaggaagaa acccggtcgc
     3481 aaaaaggcaa gaagaagcta ccagttcagc aaacgttaat ccctgttctc actactatta
     3541 taataaaata caatggaaaa taacacttca ttacaacaac aactcctcga agccggtgtc
     3601 catttcggtc acctgcgtaa gaagtggaac cccaaaatgc tgccttacat cttcgccgag
     3661 aagaaaggta tccatatcat cgatctgaac aagactacgg aaagtcttca ggaagcggct
     3721 gccgcgctca agcagatcgc caagagcggc aagaaggtca tgttcgtcgg caccaagaag
     3781 caggcgaaag agatcgttac ggaatgcgcc cgcaaggtga atatgccgtt cgtaaccgaa
     3841 cgttggctcg gcggcatgct gaccaacttc aataccgttc ggaagagtgt caagcagatg
     3901 cagagcatcg aaaagatgct gaacgacggc actttcgaca gcatcaccaa aaaagaacgt
     3961 ctcaccctca gccgggaaaa agataagaaa gagaaagttc tcggcggtat cgctcagctg
     4021 ggacgcgtgc ccgccgccct gttcatcgtg gacatcggtc atgagcacat cgcgctggca
     4081 gaagccaaac gcctgggtat aaccaccttt ggcatggtcg acaccaactg cgatcccaac
     4141 aaggtggact tcgccatccc tgccaatgac gatgcgacca agtccatcgc catcatcgtc
     4201 aactatgtca ccgctgccat tgccgaaggc cttgccgaaa gacaagcagc taaagaggaa
     4261 gacgtagaag attccggaag cgacgagaat gaaaggaagg ctgcgcgtct gcaggcggaa
     4321 gcggaaagcg aagcttcccg cggccgtggt gagaaggtaa agggcaccag ccagcctaaa
     4381 cgcaggatac cgaattccgg accgcgtcgc cccgggggac gttagtatta caccttatta
     4441 aacttaatca tatgtcaact gcaactataa cagcagccga tatcaacaag ctgcgccaga
     4501 ctacaggcgc cggtatgatg gattgtcgca aagcgctcgt ggaaagcgat ggggatttcg
     4561 agaaagcgat cgattatctg cgcaagaaag gtcagaaagt tgccgcatta cgcagcgatc
     4621 gcgaagccaa ggaaggcgtg gtgatcgcta cgaccacccc cgataataag acgggctata
     4681 ttatcaccgt agcgtgcgaa acggactttg tggccaagaa tgcggatttt gtagctttcg
     4741 cgcaaagcat cgttaacgtc gcgctgaagg agaacatcaa gacactggag gaattgcaat
     4801 ctgcaaggct ggacggtgtc acgatatccg acaggatcaa cgaccaggtt gcccgcatcg
     4861 gtgagaagat ccagctggca cgtttcgagc gtatcgacgc agaaggtgta gccgcctata
     4921 tccatggcgc ctatcgcatg agcgtcctgg taggactgtc gaagaatact cccgccgtgc
     4981 tcgaagccgg taaggacatc gccatgcaga tcgccgccat gaatcccgtt gcagtcgatc
     5041 cagattcggt ttccgccgat gtcgttgcac gcgaaaaagc gatcgttaca gagcagatca
     5101 ggaatgatcc gaagatggcc ggcaaacccg atgagatgat cgacaaaatt gccgtcggaa
     5161 aattgaacgc cttcttcaag gagaatacct taactgcaca ggctttcgtc aaggatgcct
     5221 ccaaaactgt aggcgattat ctgaaaggtg tcgatcctac tgtcaaggtg acccagttca
     5281 aaagagtaca actcggataa ttaaaaaagg tggcagtgcc accttttttt ataccttcgc
     5341 gacatgatcc ccaaatacaa gcgtgttctg ctgaaattat ccggagaggc cctgatggga
     5401 gacaagagtt ttggtttcga taacgccgtc atcgctcaat acgcacagga tatcaagagg
     5461 atcgtcgagc tcggagtaca ggtggccatc gtcatcggcg gcggcaatat ctaccgcgga
     5521 atgaatgagg cggagaccgg catcgagcgg gctcacgggg actatatggg catgctggcg
     5581 accgtgatca acgggatggc actgcaggcc ggtctggaaa aaataggtgt ctatacgcgt
     5641 ttgcaaagcg caataaagat ggagcagatc gccgaaccct atatccgccg ccgggcgatc
     5701 cggcacgtcg aaaaaggtcg cgtggtcatc ttcggcgcgg gaacaggtaa tccttatttt
     5761 actaccgata ccgcgggttc gctgagagcc atcgagatca atgcggaagt gatcctgaaa
     5821 ggcacccgcg tggatggcat ctatacggcc gatcccgaga aggttccaac cgcggaaaaa
     5881 ttcgaatcca tcaccttcca ggagtgcatt tcccgaaatt tgagggtaat ggatatgacg
     5941 gcttttaccc tctgtatgga aaaccagctt ccgatcatcg tcttcgatat gaacaaaccc
     6001 ggaaatctcc tgcgggtagt tcgcggagaa aaggtaggaa cattagtaaa agattgattg
     6061 ttaatctatt gaaagcagcc aaatactatt tggtcaaatg tttgcttatc tgggcaaacc
     6121 atagtaattt ggtcacttga tcaccaccga ctacttatgc acgagtagtt cttacagctt
     6181 cctccctcta aatattccat gaaaagcaag ccctgttgtt aacgatcatt cccccggaaa
     6241 agccatagta ttgtaaagta gtctgtttgg ttaaatcttg ttccgaaccg ttatgaacac
     6301 cttaaacaac ttgaacatca tattgggcat ggaattgacg atgagcctcc ccgttatcat
     6361 catctgtgag ttgggagctc ttattctcgg gttcaccatt cattttgtct ggaattcgaa
     6421 aaagagtctt ccgatcaacg acccctcccc ttcagccggt atcagtgaag atgacaactg
     6481 gaaactgaag tattacaacg acatggatat gcaggagaga tcgcagcagc agctgcgcga
     6541 ccgtctttcc aaagcacagg agaccgagca ggtcctgaat atcgaactcg atgagtcgcg
     6601 gcaggagctc gatagcctgc ggctggagct ggatgaagtc cgcgcgcaac tggaggaaac
     6661 ggaggcccgc ctcgtgcccc cggcaaccga agagaacggc gttccggccg atcagcaacc
     6721 ggaacccgat tacgcgggca actatcttta ccagctaaag gcggcacagg aaaaactgat
     6781 cgagcataac ggtcatatac aacgcctgct ggaacagacc cagctggtcg aagagtcgga
     6841 aaagaagaac cgcgagttga cgcagctgaa tgaagatatc aatgcccgtc tccgcgaggc
     6901 aggtcagctg ctgatagaga aagagacgga gatcagcaat ctccggcatc agcaaaaact
     6961 atcggaagaa atgacccagc ggctcgacaa agtctacgag gaatataata cgctccagga
     7021 caagctgcag aaattacagg cctatctgac acaaccctac aaccgcggta cggactacga
     7081 aggattgaaa gagtcctatt tcaaattagg caaggagcat gatgagatga agctgaaata
     7141 tatttcgatc cgtgaggaga accagcgtat cacccgcatc ctcggagaca ccgaagaaaa
     7201 actgaaggaa gccaattttc aacgtcttca atatcagaaa cggtccgcct tcctcgaaga
     7261 gctgaaccac gacctgcagg agatcgcgga gcacaacaag aagatcgaaa gtcagctccg
     7321 gcgcgtcacc gacatggaaa atctgctcgc caggatcacg gggtccggca cgatcaatga
     7381 tatcgcccag ggtatggagt gaaggatttg ccgtactttt accctccaaa attatccctc
     7441 gtgtctacta ttgccgatat tcggaaagaa tataaattac agacgctctc ggaagatgat
     7501 atactaaagg atcccttcgc ccagtttgga aaatggtggc aggaggcgat gaatgccggt
     7561 atcgaagagc cgaatgccat gaccctggcg actgccactg ccgacggaat gccggatgcc
     7621 cgcatagtct tgttaaaagg atttgacgaa agaggtttta cattttacac caactacaat
     7681 agtaccaaag gcatgcagct gctggagaac ccacgcggcg ctctcgtttt tttctggaag
     7741 gaactggaac gccaggttcg tatcggcggt ctcatcaacc tcgcccccgc gcgcatcagt
     7801 gacgaatact tcgatagccg tcccgaagga agccgcatcg gtgcctgggc ttctccccag
     7861 agcgaaccca tcagcagccg cgaatggctg gaagacaacg aaagaagata ccgtgaatta
     7921 tggcagggta aaatgatcgt gcgtcctccg cattggggcg gatatgtgct taagccggtg
     7981 aggatcgaat tttggcaggg acgtcccagt cgtctgcatg accgattcct gttcacgctg
     8041 caatccagcg gaccctggaa gatcgagagg ctggccccat aggcggggcg tctgcacccc
     8101 gcctatgaag aaagttgtta tttgcctttg gcggcagaag ttgccgattt tttggtgcgt
     8161 ttggcgggac ggctcttgcc gaacgatccc ctgaaaatct ttcctttttt ggttttctta
     8221 tctcctcttc ccatgttgaa caattttgat atatagtaaa aaatttaaaa agccccggca
     8281 ccttaaccgg tagcggagct ttgctagccg ttgagacctt tatccaaagc cccggaggcg
     8341 gcttgttcgt tagaacagac ttataatttg gcggacagtt ctttaccagc tttgaactta
     8401 gctactttct tcgccttgat cttgataaca gcgccggtct gagggttgcg accgttacgg
     8461 gcggctcttt tggacaccga aaaagtaccg aagcctacca gggtgacctt tccattacct
     8521 ttcagtgttt tggtgactgc ttcaacgaaa gaatccaggg aagcgtttgc ctgggtctta
     8581 gtaatgcctg cgtcatcggc aatttttgcg attaattcag ctttgttcat agtgtaattt
     8641 tttatttaat tagcagcaac aaatataccc gctttttgaa tccgacaaaa tttttttacg
     8701 tttgtgaccg ggcgtgaaac ccacgctaat cctcatctga cgcttacccc actgactcca
     8761 tcagcgtgcg aaatgcgatg aacccatccc cccatttctt tcgtgcctcc tcctggaacc
     8821 tgggcgcgaa gcctgtcaca aactgttcac aacgttcaag ggaggtggta aaatactgcg
     8881 tgacgaaggt gggtccctcc tcatcctcct gctccagcag gcgatacaat tgataagaat
     8941 cgaatgttcc ggtggacagt acagcaggga tctgttcctc cttcagccag gttagccagc
     9001 cttcgattat gtcccaccgt atcttgacgg taatgttata tacgatcatg aggggatttt
     9061 ttcgttccga tggcgccgac catcttcacg ctgaaataaa ttaagaaaat caacaccgta
     9121 gaaaggatca cccaaagaag gaccccggac tggcgctcgg ccgcggccgt ctttttattc
     9181 actgttctgg cggcagcctg ggatgccggc tgacctttcc cggtcagata ttgcaggggc
     9241 cccggtgtca ggtagtgcat ctcgccgatc aggctgtcag tgaaatattt gaggtcatag
     9301 tcgggcacag cggctgcagg attgccagtc aatacgaagt agccttctcc ggcagtcaaa
     9361 taggatacca tatacctgca tatctgggaa caggctatcc tgctgatgac aagaggaggg
     9421 ttgtcttcat tggcaatatc gatgtgtaag caatgggttt tagttgaagg gatatgaaac
     9481 aggatatgcg cagggtcgat catgatacgg gtaaccggct cccattgccc tggcacaggg
     9541 ctctctgccg tgatatatgc cgaccgcttg tagtattgcg ggctattgat taccagcacc
     9601 accctgtcga tctcgtatgc ttctgtgaat cttagtgtaa tgtaagaatg tttatcgctg
     9661 ctgtcctttt gttcaggtgc cacctcggga atgttcagga gcgcacgggg tatgtctttc
     9721 agcacatacg gcacgaatcg attgtccggc ccaagaatgc gaagatcccc gagatcaggc
     9781 cggctctttg ccaccagcga cgggggcaag agtacccagt aaaaagcgtc gctctttatt
     9841 gtatccagcg gggcccggta atgaaagcta tcctgcgccg caccatgcag cgatagcagc
     9901 accaggccca acggcaacca tctgtcagac catcttcttt tcttcatcct cgatgatgat
     9961 ttttttgagt cgttgataca tgaatgatac caccagcagc aatacgccca gacagaagaa
    10021 cgcggcgatc ttccccgcaa caggaatgtt acggatatcg aaaaggaaca gcttcaaaag
    10081 tgtaatagag aacaagacca gcgaaactat tctcagcggt tggaatttgt gccgcatacc
    10141 catccacatg aaggtgaagg agcacaggcc ccacaggatc ggcagcacgg ttttgtcgta
    10201 tacacgcccc agttccggaa gatcgttctg tttcgaatag aagagcgaat tgaccaggag
    10261 ttgtccttcc gcgctgagca ggataacggt tatgatacag atcagccagg tgaacagcgc
    10321 tggatccatg cttagtgtgc gggcccgggc cgctacgacc aattgccaca taatccacac
    10381 gatcaggaca tcccctgtcc agtgtgcgat aaaatggacg ccatagcggt ttttatacaa
    10441 catggtctcc tgcagaccga aaatatatat cagcgagacg agatagagga atatcgcggc
    10501 gtattgtaac accgcgttca tcagccggaa atcgtgtttt ccacgggtaa cggccgtaag
    10561 caaggtgata aaaagataag tatacagcag gagatagagc ggcgccagat cgatgccatg
    10621 gaagtaaaag gcgaactgat gagcgatctc caatgcgcca tcggcgaaaa gcaggaccag
    10681 gccggcgatc agcgctatcc tggcaatcgg ggttcttttt ctcagataat agatcgctcc
    10741 gcagcaaagg gccgaataaa gcccggtcat gaagccctta ttgaagacga tcggtagcgc
    10801 cccaggacca ttggcggcag gcatgtcata aattacgggc ccgtaaatgt tgaaccagtc
    10861 cataagcaga ctgataagca tggctatcca gacgagcagc gccgacagcc ggatgaggtt
    10921 tatcccggaa cgcacgaaaa gccagcttaa caataccgac tcggatgccc agaatagggt
    10981 gatatagtgt ccatgcaatt gcaatggcgc cgtcagtgat acgaaggaca atgtgatacc
    11041 taccagcaaa tagaggatat tcttgtcgac ggtccgctgc cgaaaaagga accacgtagc
    11101 gacgaggtgg aagacgccca tgacggcgca gaacagtcct ttataccggt agagacccaa
    11161 ctgttcgata aaatagagac tcgccgagaa gtacagacag gtattggaca gcaggatgat
    11221 aaagtccgag gcgatgaagg gcctggtctt ccggatcgta tgcgcgatat tgatggcgaa
    11281 gaacagcagg taaaatgtcg ttgcgaagct ccatcctccc ctgtagacgg cggaagacgc
    11341 gtcatagggc aggctcacca gccaggaaaa gaaaagcagg gctgtgaagc cgaaggccag
    11401 gatgttcagc agccgccagg tcttatgata cgcaatgacc agcaatcccg tattcaggat
    11461 cagcagatag gagaaaagga ccacataatc tcctcttccg ttgctgacca ggaatggcgt
    11521 gatgaatccg cccgccagtg cgatgatcgc aagctcctgc cggtcgtaca acagagaaag
    11581 gatcaccgag aaagccgtga tgacgagcat tatgaggaag gctgccgtct gcccgaacag
    11641 ctggtactga tgataggcga gcgcaatagt acaatacaac actgccagcc ccccgcccac
    11701 cagtactgag ctaaaagcct tataccggtg acgcaaccaa tgggcaatgc cgatcaggat
    11761 gccaccgcac agaataccga caccaacgcg tcctgccggg ccgatccagt cgttgtcgat
    11821 ggcatatttt acgaagaaac cgatggccag cactaaaatg gcgattccga tcttgctcac
    11881 aaggttttcg ccgatgaatt tttccaggtc gggatgacgt tcgaaaaacc cgggctccgg
    11941 ctcctgcgca ggttcgggcg tacgcaccgc cggtgtttgg ggacgggctt cggggatggg
    12001 tctgaaaggt tccgtttcct gaggcggcgg aacaggatag cgatgcggtt cggcaagccc
    12061 gggatatcga tcaggttcgg caggcgtctg gggcctatgc ggctcggggg gtgcaggcct
    12121 tgcgaccgga gtgtccgccg gcttttcctc gcgaagccgt ctgatctcat gctcaagcgc
    12181 gtcgaatcgt cccagcagtg tcgtacgcat caccatcacc agcacaataa ggaggatgac
    12241 taaaatgatg agtatttctt ccatgcaggg gggctggtta atggataaag atactattct
    12301 accgacaatt cgaggaatac gggacagtgg tcgctttgtc gtacatcggg gtatatttcc
    12361 gccttaagca gctgcccttt cagcgggtcg gtcacgctga tataatcgat cctccatcct
    12421 ttattctgct cacggacgcc tggaaaacgc tggctccacc aggtataccg atgtggttcg
    12481 ggattcagat gacgaaaact gtctatccaa ccgctggtga tgaacttatc catccaggcc
    12541 cgctcatccg gaagaaaacc ggaattgttc ttattgcctt tcggatcgtg gatgtcgatc
    12601 tccctatgtg cgatgttata atcgccgcaa aggatgagcc gtggatattt ctccttcaac
    12661 gttcccaggt aaccgaagaa ttcatccagc catcgatatt tatatccctg gcgttcttcg
    12721 cccgaagtgc ccgaagggaa ataggcattg atcaaacgga tatccccgaa atccagctgg
    12781 atcacccggc cttcatcatc gctgacggta tgtcccgtcc catattctac gtgatccggc
    12841 cgcaccttgg taaagaccgc tacgccgctg tacccctttt tcttcgccgg gaaccaataa
    12901 tcatgaaaac ccaattcggt gaacaatgcg tgcgccacat tgtccctatg cgccttcgtt
    12961 tcctgtatgg cgacgatgtc ggcgacgtcg gtcttcagcc actcgatgag gcccttattc
    13021 atggcggcgc ggataccgtt gacattgtag ctgacgatgc gtaatgaaga cataagcgct
    13081 atattagagg cgcaatttaa ttcaaaatgg accacacaga ccttataata ccttccaggg
    13141 agacgggata tatcgatgga ccccggagcc gtacctatga gcttcggttc gcctggggca
    13201 tattccgcga gctgatccgg ggattccgga ccctgcattt cgtcggaccc tgtatcaccg
    13261 tattcggatc ggcgcgtttc aaagaagacc atccgcatta ccagtcggca cgcgagatag
    13321 gacgtcagat cgccggtctg ggttatacca cgatgacggg gggaggaccg gggatcatgg
    13381 aagctgctaa ccgcggggct ttcgaggcgg gtgggatgtc ggtgggatgc aatatcaagc
    13441 tgcctttcga gcaaaaaccc aacccttatg tacagacctc gatcaccttc gatcattttt
    13501 tcatccgcaa ggtcatgctg gtgaaatatt cctatgcatt cgtgatcatg ccagggggat
    13561 tcgggacgat ggacgaattc ttcgggaccc tgaccctggt tcagaccaag agtcttttgc
    13621 agtttcccat cgtgctgttc ggggccgaat attacaaacc gttgttcgcc tatatggaag
    13681 atatggcccg tgagggaaca gtctcgaaag aggatctctc gctcgtcctt ctgaccgaca
    13741 gcatcgccga ggctatccgg cacatcgata ccttcatcaa aaagaattat ccgcaaaaac
    13801 cgcgtcgccg caaatggtgg ctgttcgagc caagacccgc attgcctctg gctgggaaag
    13861 cctgaggctc ttgcgcgaca ccggacatcc aggcgactgg ctacttccgg ctggtcggaa
    13921 tgtcgatgcc caggctcagg atcatctcat acgtgccgaa tgcctgcctg gccttgccga
    13981 ggtaaacatt gaccccggaa taacgggcat agtcgaattt gttggaatat tcgagataga
    14041 gatagcggaa aaaggttgcg cggatgacgc cctcgaatcc cgtattccat ccgccgaact
    14101 ggaaaccctg gtgattgggg ttaccgaaga ggctgttttg agtatgcggg atgaccggtc
    14161 caacacccag cttcccgtag agatccagct ttagcttttc gtgacgggtc gtcagtacat
    14221 ggtagcgttt ggtcacgttg aaaagcagga agtttgcccc gttgttgaga taataataga
    14281 atccgttggc tgagttgaag gcgatcgagc tatccaccgg gtgattttcc aacttccctt
    14341 ttatatggac atcctggtct gctatgatat atttggtgtg gtcgaaattg atctcaaatc
    14401 cccatccccg tttttcatcg agcaggaaac cgagacggta attgtactgc ggaatggaga
    14461 gctgtttggt caggatcgcc tcatcccaac ccgggtgatc atgcgctttt acatgttcga
    14521 gcgtatagtc atttcccagc gcagactgcg acacgtgcag gctgctatgg gtataccatt
    14581 ccttattgta tccccaggag acatacactt cctttataag tttgttcttt ttcttcttcg
    14641 gaggctgttg ttgcgcgaag ctcaaaagag gcagggagag aatcaaaata catagggcaa
    14701 ctctggggta cacgacgcca ctggatctca taccggtcta ttatgcttgt agtaagatat
    14761 tgttatagcc ggtgcgaaac tacggaaatt tcatcataca tcatactcca ggacgggtct
    14821 aagccatctc tcggcttcct cgacgggcat ccctttccgt gcggcatagt cgatcacctg
    14881 gtccttttca atcttgccca cgccgaaata cttactttcc gggtgcgcga gataccagcc
    14941 g
//
LOCUS       DASYSV010000048         5246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1676898, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000048 DASYSV010000000
VERSION     DASYSV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676898"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VGQ51_01510"
     CDS             complement(<1..1033)
                     /locus_tag="VGQ51_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEV9035259.1"
                     /translation="MRLFTKYSRVNIVASILALVLGSIGYFFTVRYVLVHELDDTLRI
                     EEAEILDNVHRRDRLPDPANYVDQQIQYNPATREVPRKFHQTTWNDLVATAGKPAGAR
                     NHDQDHPFRELVFSIRVGGQLYTVSIAKSEEETEDLLMLIMVITAGMIVILLGVLFLA
                     NRLLLRRIWQPFYRTLDNIRAFNLSTRESLPVPDTDIEEFHSLDEAVRQMTDKIIKDY
                     EILKNFADNASHEMQTPLAVINSKLDLLIQDIGLGEAHHRPVQAMYDAIGRLRQLNQS
                     LLLLTKIENNQFVDTRSIDLALLIGDKLHQLEDPLKDRHLRVYTELDHRPLPINGYLA
                     DILLNNLLTN"
     gene            1089..1637
                     /locus_tag="VGQ51_01515"
     CDS             1089..1637
                     /locus_tag="VGQ51_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV9035260.1"
                     /translation="MSSRREFEIAFVGLKAGIHEFTYRIADKFFEAYQQQDFRNCDAQ
                     VKLTLDKKNSFMLLKFEVGGKVEIACDRCGNMLPLDLWDEFNILVKLVEDPEIMNQQE
                     EDPDVYYISKGESHLHLSDWIYEFINLSIPMQRMCKPEEMGGPHCNREVLEMLKELDR
                     EKSQPGNPLWKGLEKFKDLGNG"
     gene            1670..1861
                     /gene="rpmF"
                     /locus_tag="VGQ51_01520"
     CDS             1670..1861
                     /gene="rpmF"
                     /locus_tag="VGQ51_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV9035261.1"
                     /translation="MPNPKRRHSQQRGAKRRTHYKAEKVTLSKDSTTGEMHVRHRAHV
                     SEGKLFYKGKVVAENSPIK"
     gene            1874..2812
                     /gene="plsX"
                     /locus_tag="VGQ51_01525"
     CDS             1874..2812
                     /gene="plsX"
                     /locus_tag="VGQ51_01525"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV9035262.1"
                     /translation="MNIGLDMMGGDYAPLEAVKGIQTWLQEAGSATLWLIGDESLISP
                     LLQEYHIPAASVRVVHAAQTIDMHEHPTRALKEKPQSSIAVGFHLLASGKMDAFISAG
                     NTGAMLVGALYSLKPIEGIHRPTISTVIPKENGKTGVLLDVGLNADCKAENLNQFAVL
                     GSLYARYILGIDEPRVGLLNIGEEEGKGNLLAQAAYPLLAANPHIRFIGNIEGRDLLL
                     DKADVMVCEGFTGNVILKLAESLFEITSRHQTESEFFDRFDFEYYGGTPLLGVTKPVI
                     IGHGISHGKSFLNMLRLAQKMLETDLLGKMKESFLS"
     gene            2812..3741
                     /locus_tag="VGQ51_01530"
     CDS             2812..3741
                     /locus_tag="VGQ51_01530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV9035263.1"
                     /translation="MRAFFQYFASWLRSSSRPALLITIFFVASLVAANYTIGIEWRLQ
                     ALPWYLALVGFFVLFSMVLALVWGLQYEWGWKKQGYFKPKMVLLLALAALFFSFKMVH
                     WDIMPLLPRRLVYPWTRYVLLVLQLPLKLLLLFIFLWICRRLDLIGSLGSIGLTAKDF
                     KPAPYFGMLLLVVPLVALASTQHDFQAVYPKVRNLAFMNAYVHPAWPWKLAYEISYGL
                     DFLGIELFFRGLLVIAVLRYAGTDAILPMAAFYCTIHFGKPMGECITAFFGGLILGVL
                     AARTRTIYGGLIVHLGLAWLMELGGWLGHLWMH"
     gene            complement(3751..>5246)
                     /locus_tag="VGQ51_01535"
     CDS             complement(3751..>5246)
                     /locus_tag="VGQ51_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9035264.1"
                     /translation="STSRMKEIGIRKVLGGLRRQLRIQFLTEAILLAMASTVVALLLY
                     VLLFPLAGDMLGRGLPSLSALPVAAWGLILLFALFTGWLAGLYPATLLSSLASVDALK
                     ARVGTVEEKTLLRKGLVGFQFGTAAVAFIGVIIVSRQIRLFFSDRLGYNKDYMVSTPL
                     PRDWSAQGVGRMEGIRNIFAAMPGVKDVALSYEIPNGMNGGGFGAYRAGGDSSSPVIA
                     DALVTDANYAATYQIPLAAGVFFHLPGEDDSRDSTRLVLNETASKALGWRSPREAIGQ
                     PLRLVGIPGKVFTVSGVVRDFHFGGMNRPINPVIFGPVSLFITYRYLSFKLEPGNIGS
                     AIAALQRQWVRLMPGSPFEYKFMDESLEALYTNERRLQKAASTAAALMAVIVLLGVIG
                     LVSGSLRRRTKEMAIRKVVGASVPGIIRLFIREYLPVLLVAGGTASPLAWWIMHRWLD
                     EYATRITITVWPFVTAIVCLAVVIVVLIIAQTLHAARANPIDALRSE"
BASE COUNT         1197 a         1450 c         1411 g         1188 t
ORIGIN      
        1 cattggtcag gaggttgttg agcaggatat cggcgagata tccgttgatg ggcagcggcc
       61 ggtggtccag ttcggtgtat acccggagat gacggtcttt cagcggatct tccagctggt
      121 gcagtttgtc tcctatcagc agggcaagat cgatggacct ggtatccacg aattgattgt
      181 tctcgatctt ggtgagcagc agcagcgact ggttcagctg acgcagccgg ccgatggcgt
      241 cgtacatagc ctgtactggc cggtgatgcg cttcaccaag tccgatatcc tgtatcagca
      301 ggtccagttt ggagtttata acggccagcg gcgtttgcat ttcatgcgat gcgttgtccg
      361 cgaaattctt cagtatctca tagtctttta tgatcttatc cgtcatctgg cggacggcct
      421 catcgagact gtgaaactct tcgatatccg tgtcgggaac aggcagcgat tcccgggtgg
      481 acaaattgaa ggcccggata ttgtccaggg tccggtaaaa gggctgccag atacggcgca
      541 gcagcaaccg gttggccaga aaaaggaccc ccaggaggat cacgatcatc ccggcggtga
      601 tgaccatgat cagcatcagc aggtcttcgg tctcctcctc ggatttcgcg atggatacgg
      661 tgtataattg ccccccgacc cggatggaaa aaaccaattc ccgaaaagga tgatcctggt
      721 catggttccg cgccccggcc ggcttcccgg ccgtcgccac caggtcgttc caggtggtct
      781 ggtggaattt tctcgggact tcccttgtgg ccggattata ttgtatctgc tggtccacgt
      841 aattggccgg gtccggcagc cggtcacggc ggtgcacgtt gtcgagtatc tcggcttctt
      901 cgatccttag cgtatcgtcc agctcatgca ccagcacata cctgacggta aagaaatacc
      961 cgatgctgcc caggaccagc gccaggatgc tggcgacgat attgacacgg ctgtatttgg
     1021 taaaaagcct catttgttgt aatttagata ttttctccct acctttgcaa gctctttaaa
     1081 aagcagtcat gagcagccgc cgggaatttg aaatagcgtt tgtgggccta aaggctggta
     1141 ttcatgagtt tacctatcgg attgccgata agttctttga ggcctatcag caacaggatt
     1201 tccggaattg cgatgcacag gtaaagctga cgctggacaa aaagaactcg ttcatgctgc
     1261 tgaaattcga ggtcggcggc aaggtcgaga tcgcctgtga tcgttgcggg aatatgcttc
     1321 cgctcgatct ttgggatgag ttcaacattt tggtcaaact ggtggaggac cccgagataa
     1381 tgaaccagca ggaggaagac cccgacgtat attatatatc aaaaggggaa agccatctgc
     1441 acctatccga ctggatctat gagttcatca atctgagcat ccctatgcag cggatgtgca
     1501 agcccgagga aatgggcggg ccgcattgca acagggaagt tctcgaaatg ctgaaagagc
     1561 tcgaccggga gaaaagccag cccggcaacc cgttgtggaa gggtctcgaa aaattcaaag
     1621 acctcggcaa cggttaataa gtataaagat tttttaaaat agaagtgata tgccaaatcc
     1681 gaaaagacgc cattcccagc agcgcggagc aaagagaaga acgcattata aggcggagaa
     1741 agtgaccttg agcaaagaca gcaccaccgg tgagatgcac gtacgccatc gcgcccatgt
     1801 cagcgaaggc aaattattct acaaaggaaa agtcgtcgcc gaaaactctc ccatcaagta
     1861 atcagagagt ccaatgaaca ttggtctcga catgatgggg ggcgactatg ccccgctgga
     1921 ggcggtgaaa ggcattcaga cctggttgca ggaagccgga tcggccaccc tgtggctgat
     1981 tggtgatgaa agcctgatat cgccattgtt acaggaatac catattcctg ccgcctcggt
     2041 ccgggtcgtt cacgccgccc agaccatcga catgcatgaa caccctaccc gcgctttgaa
     2101 agaaaagccg cagtcttcca tcgccgtagg ctttcatctg ctggcgtccg gtaaaatgga
     2161 cgcctttatc agcgccggca acaccggggc catgctcgtt ggggcgctct acagtctgaa
     2221 acccatcgaa ggcattcatc gacccaccat ttccaccgtc ataccgaaag agaacggcaa
     2281 gacaggagtc ttgctcgacg tcgggctgaa cgcagactgc aaggcggaga acctgaacca
     2341 atttgccgta ctcggctcat tgtatgcacg ttatatcctc ggcatcgacg aaccccgtgt
     2401 gggtctgctg aatatcggag aagaagaagg taaaggcaat cttctcgccc aggccgctta
     2461 tcccctgctg gctgccaatc cgcacatccg attcatcggc aatatcgaag gacgagacct
     2521 cctgctcgat aaggcagacg tcatggtatg cgaagggttc accggcaacg tcatcttaaa
     2581 gctggccgaa tcgctcttcg aaattacgag tcggcatcag acggaaagtg aattcttcga
     2641 caggtttgac ttcgaatatt atggaggcac tccgctgctg ggcgtgacca aaccggtgat
     2701 catcggacat gggatctcgc acggcaaatc ttttctcaat atgcttcggc tcgcacaaaa
     2761 aatgctcgaa accgacctgt tgggcaaaat gaaagaaagc ttcctgtcct gatgcgggca
     2821 tttttccaat actttgcctc ctggctgcgc agcagctccc gccccgccct cctgatcacg
     2881 atcttctttg tcgcctcact ggtcgccgcc aattatacca tcggcatcga atggcgttta
     2941 caggcgctgc cctggtatct ggccctggtg ggttttttcg ttttgttcag catggtgctc
     3001 gcgctggtct gggggctgca atacgaatgg ggatggaaaa aacaggggta tttcaaaccg
     3061 aaaatggtat tgctgctcgc gctggcggct ttgttctttt cgtttaagat ggtccactgg
     3121 gacattatgc cgttgttgcc gcggaggctc gtctatccct ggacgcgata tgtgttgctc
     3181 gtcctgcagc taccgctcaa gctgctgctg ctgttcattt tcctttggat atgccggcgg
     3241 ctcgacctca tcgggagtct cgggtcgata gggcttacgg ccaaagattt caaacctgct
     3301 ccgtattttg ggatgcttct tctggttgtc ccgctggtgg cgctcgcgtc tacccaacat
     3361 gattttcagg ccgtttatcc gaaggtgcgg aacctggcct ttatgaatgc ctatgttcat
     3421 cccgcctggc cgtggaagct ggcgtatgaa atttcctacg gtctcgattt tctgggcatc
     3481 gaactttttt tccgtggact gctggtcatc gcggtgttgc gctatgccgg caccgatgcc
     3541 atacttccaa tggccgcttt ctattgcacc atccatttcg ggaaaccgat gggagaatgt
     3601 atcaccgcct ttttcggcgg tctcatcctc ggcgtactgg ccgcccgcac ccgcactatc
     3661 tatggaggtc tcatcgttca cctcggtctg gcctggctca tggagctggg tggatggctg
     3721 gggcatttgt ggatgcattg agcacactca tcactcactt cttaaggcgt cgattggatt
     3781 cgctcttgcc gcatgcaacg tctgcgcgat gatcaatacc acgatgacta cagccagaca
     3841 tacgatcgcc gtcacaaacg gccaaaccgt gatcgtgatc ctcgtcgcgt attcatccag
     3901 ccagcgatgc atgatccacc aggccagcgg agaggcagtc cctcccgcca ccaataggac
     3961 cggcagatat tcccggatga acagacggat gatgcccggc acgctggcgc ccacgacctt
     4021 gcggatggcc atttcctttg tccgccggcg aagacttccc gacaccagac cgatgacgcc
     4081 aagcaggacg atgacagcca taagtgcggc tgccgtcgag gcggccttct ggagccgccg
     4141 ctcgttcgta tacagcgctt cgaggctttc gtccatgaat ttgtattcga agggcgagcc
     4201 gggcatcagt ctcacccact gcctttgtaa tgcagcgatg gcagagccga tattgccggg
     4261 ctccagctta aaggacaggt agcggtaggt gataaataga ctgaccggcc cgaatatgac
     4321 cggattgatc ggcctgttca ttcctccgaa atggaaatcc ctgacgactc cgctgacggt
     4381 aaagactttc ccgggtatgc caaccagccg taagggttga ccgatcgcct cgcgtggaga
     4441 tctccatcca agcgccttcg atgccgtctc attcaacacc aaccgggtag agtcgcgcga
     4501 atcgtcctcg ccgggcaaat gaaaaaagac accggcagcg agcggtatct ggtaggtcgc
     4561 cgcatagttg gcgtcggtga ccagggcatc ggcgatcaca gggctggaag aatcgccccc
     4621 cgcccggtaa gcgccaaagc cgcctccatt catcccattg ggtatttcat aggagagagc
     4681 gacgtcctta acacccggca tcgcggcgaa aatgttcctg ataccttcca tccgccccac
     4741 gccctgcgcc gaccaatcgc gcggcaacgg tgtggatacc atataatcct tattgtaacc
     4801 caaccgatcg ctgaagaaaa gcctgatctg ccgggaaacg atgatgactc ctataaaggc
     4861 cacagcagcg gtgccgaatt gaaacccgac cagtcctttt cgaagcaatg tcttttcttc
     4921 cactgtcccg acccgcgcct tgagcgcgtc taccgaggcc agcgatgaca gtaaggtcgc
     4981 gggatataaa cctgccagcc agccggtgaa caacgcaaac aataagatca gcccccatgc
     5041 cgcaaccggc agcgccgata aggatggcag ccctcttccc aacatatcac cggccagcgg
     5101 gaacaacaag acatacagca acaacgcgac tacagtagag gccatcgcca ataggatggc
     5161 ttcggtgagg aactgtatcc gtaactgccg gcgcaaacct cccagcactt ttcggatgcc
     5221 gatctctttc atccgcgagg tcgatc
//
LOCUS       DASYSV010000049         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_782512, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000049 DASYSV010000000
VERSION     DASYSV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782512"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VGQ51_01540"
     CDS             complement(<1..516)
                     /locus_tag="VGQ51_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9035265.1"
                     /translation="MRIAKASPTQLQFLIWCFVFLFSLLTFLPEEGFWQALSWSALNC
                     FFYAVIIYGNILFLFPRLYEKEHLGWYILASIALLAGVGIARAIIYPAIYNRWFAKMK
                     EIVTIWTYLYYTIAGFVIFLLSFVFRIALAYFRIKRQAEQILGQKSQAELNLLKSQVQ
                     PHFLFNTLNNIY"
     gene            805..2196
                     /locus_tag="VGQ51_01545"
     CDS             805..2196
                     /locus_tag="VGQ51_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEV9035266.1"
                     /translation="MITTKITLKRFLPFFLLAAVATAAVFIPSIAPKLADGKYSSADV
                     AWMLVATALVFLMTPGLAFFYGGMVNRKNVLSTMMKSVVAAGVVGVLWIVVGYSLCFG
                     DSIHGVIGNPLTHLFYKGVVSGDPFSPAPTIPKALFSMFQLMFAIITPGLVVGAIAER
                     MRFSAYVLFTVLFSLLVYAPLAHWSWHPDGFLAKMGALDFAGGTVVHISAGCAALAGA
                     IVLKRRKVHINHQETHPANIPYVLIGTGLLWFGWFGFNAGSAFGANQLAVSAFSTTNT
                     AAAAAGLSWMFFDVLRGKKPSVVGFCVGAVVGLVAITPAAGFIAIPQSIFVGVLAAVI
                     SNMAVHFKATKTVLDDTLDVFPCHGIGGMVGMLLTGIFATKAVNINGANGLLYGNPSF
                     FLTQLKAMGCAVGYSFVVSYGIFKLVNFIVPLRVSEEEEEEGLDASQHDEKYTQGTLL
                     VATPKGLEEHEVM"
     gene            2257..2913
                     /locus_tag="VGQ51_01550"
     CDS             2257..2913
                     /locus_tag="VGQ51_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEV9035267.1"
                     /translation="MITENVLVEARKLWDYHHLHHVPAQTDCILVLGSHDLRVADRGA
                     DLYLQGWAPIIIFSGGLGNVTKGIWKDPEADRFARIALDKGVPSEAIFVENESTNTGE
                     NILFTGRMLEEKGLHPQSFLLVQKPYMERRSYATFKKHWPDKDLIVTSPQIAFEDYPT
                     EEISMQRVIEIMVGDLQRIRIYPEKGFQIPMEIPDEVWAAYEMLVAWGFTKHLVKDEA
                     "
     gene            complement(2917..>3561)
                     /locus_tag="VGQ51_01555"
     CDS             complement(2917..>3561)
                     /locus_tag="VGQ51_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9035268.1"
                     /translation="DHQMPGMDGITLVGKIKESLKDVPQPFILMLSSLDKGMCIDDAQ
                     RMGIDLFLSKPVKLHELNGILQSIFGSPREQPQETPATPEIRKLGTINTSILVAEDDP
                     VNMLLISEVLAKMGFTVIKATDGKQVLEQLGSHIPAIILMDVNMPEMDGLEATQIIRT
                     MPQPQGNIPIVALTAAAMSEDRERCLLAGMNSFITKPFKLQELEDVLRKYVDAA"
BASE COUNT          796 a          830 c         1015 g          920 t
ORIGIN      
        1 atagatattg ttgagggtat tgaagaggaa atgcggttgg acctgggatt tgagcagatt
       61 gagctcggcc tggcttttct gcccgagtat ctgttctgcc tgacgtttga tccggaaata
      121 ggcgagcgcg atgcggaaga cgaagctgag gaggaagatt acgaaaccgg caatagtgta
      181 atagaggtag gtccagatgg tgactatctc tttcatcttt gcgaaccagc ggttatagat
      241 agcagggtag atgatggcgc gggctatgcc gacgccggcg agcagtgcga tcgaggcaag
      301 aatataccag ccgagatgtt ctttttcata gagacgggga aagaggaaca ggatattccc
      361 atagataatg accgcataaa agaagcaatt gagggcgctc caggaaaggg cttgccaaaa
      421 gccttcttcc ggcagaaagg tcagcaggct gaacaggaag acgaaacacc agatgaggaa
      481 ctgcagctga gtgggagatg ccttggcgat tcgcatggga cgaaattact atccttttga
      541 cctgccgggg caggatttca acagcggggc aatgcggttc aatgatcatt ctttgaagta
      601 atagcggctt ttgagagatt ttttagcgtt ttttcgaatt tttcatgaac atcgttttgc
      661 ctgtatttct gtatttaatt gacaattaaa ttgttgcgta cagtgtgaca aacatgtgca
      721 ttttaattat atgttgttgt tcggcgcaat ctcaaaatat ttgtatcttc acttaggatt
      781 taaatgtatt aatatttaat taatatgatt acgactaaga tcactttaaa gcgcttttta
      841 cctttttttc tactggcggc ggtagccaca gcggcggtgt tcataccgag tatcgccccg
      901 aagctggcag atggcaaata cagttctgcc gacgtggcct ggatgttggt cgccacggcg
      961 ctggttttcc tgatgacccc tggtctcgcc ttcttttatg gggggatggt gaacaggaaa
     1021 aacgtgcttt ccacgatgat gaagagtgtc gttgcggcag gggtggttgg tgttttgtgg
     1081 atcgtagtcg gctatagtct ttgtttcggg gactcgatac atggggtgat cggaaatccg
     1141 ctgactcacc tgttttataa aggggttgtt tccggcgatc ctttttcgcc tgcgccgact
     1201 atacccaagg ccttattctc catgttccag ctgatgttcg ccattattac cccggggctg
     1261 gtggtaggcg ccattgcaga gcgtatgcga ttctcggcct atgtattgtt caccgtactg
     1321 ttcagcctgt tggtttatgc tccgctggcg cactggagct ggcacccgga tggctttctc
     1381 gccaagatgg gagcgctcga cttcgcaggt gggacggtgg tacatatatc cgctggctgt
     1441 gcggccctgg ccggcgccat cgtgctcaaa cggaggaagg tacatatcaa tcaccaggag
     1501 acacatccgg ctaatatacc ttatgtattg atcggtaccg gtctgttatg gtttggctgg
     1561 ttcgggttca atgccggatc ggcttttggc gccaatcaac tggctgtgtc agccttttcg
     1621 actacgaata cagcggcggc agctgccggt ctttcctgga tgttcttcga cgtgctgcgg
     1681 ggaaagaagc cttctgtggt tggtttctgc gtgggtgccg tggtcgggct ggtggcgata
     1741 acacctgcgg ccggctttat cgcgatccct cagagcatat ttgtcggagt actggcggct
     1801 gtcatttcaa atatggcggt tcatttcaag gcaaccaaga cggtcctcga tgacacgctg
     1861 gacgtgtttc cctgtcatgg catcggcggt atggtaggga tgttgctgac gggcattttt
     1921 gccacgaagg cggtgaacat caacggagcg aatggtctgc tgtatggtaa tccatctttt
     1981 ttcctgacac agctgaaagc gatgggctgt gcggtaggat atagctttgt agtctcctat
     2041 ggcatcttca aactggtgaa ttttatcgtt ccgctgcgcg tcagtgaaga ggaagaagaa
     2101 gagggactgg atgccagcca gcacgacgaa aaatatacgc aaggcacatt gttggtcgca
     2161 accccgaaag gactcgaaga acacgaagtc atgtaagaat caggctttcg atagggggcc
     2221 ggcgcaagcc ggccctttcg cttatctttg ccccgtatga tcacggaaaa cgtactggtg
     2281 gaggcaagga aactgtggga ctatcatcat ttacatcatg tgcccgcaca aacggactgt
     2341 atcctggttc taggcagtca tgatcttcgg gtagccgatc ggggtgcaga tctgtatctg
     2401 caaggctggg cgccaatcat catcttctcc gggggactcg gcaatgtcac taaagggatc
     2461 tggaaggatc ccgaggcgga tcgttttgcg cgtatcgccc tcgacaaggg tgtcccgtcg
     2521 gaagccatct ttgtcgagaa tgaatccacc aatacaggcg agaatatcct gttcaccggg
     2581 cggatgctgg aagaaaaggg attgcatccg caatcgttcc tgctggtaca gaaaccttat
     2641 atggagcgcc gcagctatgc cactttcaag aaacactggc cggacaagga cctgatcgtc
     2701 acttctcccc agatagcgtt tgaagactat cctaccgaag agatctctat gcagcgggtg
     2761 atcgagatca tggtagggga tctgcagcgt atcaggatct atccggagaa aggctttcag
     2821 ataccgatgg aaatcccgga tgaagtctgg gcggcatatg agatgctggt ggcctggggg
     2881 tttacgaagc acctggtgaa agacgaagcc tgacggttac gccgcatcca catattttcg
     2941 gagtacatct tccagttcct gcagcttgaa tggcttggtg ataaaactgt tcataccggc
     3001 caggagacaa cgttcgcggt cttcgctcat cgccgccgcg gtcagcgcca caatgggaat
     3061 attgccttgt ggctgcggca tggtccggat gatctgggta gcctccaacc cgtccatctc
     3121 cggcatattc acgtccatca gaatgatggc agggatatgg ctgcccagct gctccagcac
     3181 ctgtttaccg tcggttgcct tgatgaccgt gaagcccatt ttagcgagca cctccgagat
     3241 caggagcata ttgaccggat catcttccgc cacgaggata ctggtgttaa tggtccccag
     3301 cttgcggatc tccggcgtgg cgggtgtctc ctgaggttgc tcccggggcg atccgaatat
     3361 cgattgcaaa atgccattca gctcgtgtag tttcaccggt ttggagagga acaggtcgat
     3421 gcccatccgc tgagcatcgt cgatacacat ccctttgtcc agcgaggaca gcatcagaat
     3481 gaatggttgc gggacgtcct tcagtgattc cttgatcttt ccgaccaggg taataccgtc
     3541 catccccggc atctggtgat c
//
LOCUS       DASYSV010000050         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1165637, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000050 DASYSV010000000
VERSION     DASYSV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165637"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VGQ51_01560"
     CDS             <1..1403
                     /locus_tag="VGQ51_01560"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEV9035269.1"
                     /translation="VGDYLNEHASIVAGLWLLCLTFQLVRLTGGLYQMRRFRRKGIHP
                     PGIEWVGRLATLCRVAGVRKKVLLLQSELVSMPVTFGFLKPTILIPFGMIAGLPADQL
                     ETILLHELAHIRRNDYLSNLLLYITESVFFFNPAFRWMASRIRQEREACCDDIVLADT
                     PNRNSYFDALIAFRVQAMAQEGYPLQLGIGKTDLLWRIRRMLDKENKKLHVMEKALLS
                     VGLMIVLAIGLVSMNEWQAPPPAVTSAPAEVSSDTAPAIKVPYPSISKSSDIEGARKT
                     CKISATDAEGNTFQLTKTNGEITEMIINGNVLSPADYDRYEYIFDKIERHREARENAQ
                     SDEATEATEQVEATEATEATEATEATETTEATEATEAPETTEATEATEVTEAPAPASN
                     RWIHCIIKDLTRNGIIANEDPLSFTLDKDHLIVNGVRQPDEVYRLFKEKYINNPKDHF
                     DYSRDGGSTHVEVWVE"
     gene            complement(1406..>2592)
                     /locus_tag="VGQ51_01565"
     CDS             complement(1406..>2592)
                     /locus_tag="VGQ51_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HEV9035270.1"
                     /translation="TIFSLWDTYRTFHPLMTIIQPERVNDFVNTMLAIYQQQGKLPVW
                     HLQGRETDCMVGYSAVPVIADAWLKGFHGFDAGLALTAMKATSTRDDYGLKYLKQDGF
                     IPADKEVEAISKGMEYAIDDWCIARMASQMGRTEDAATYTRRSQLFTRYFDPSTRFMR
                     PVLADGKFITPYSPFLTKNYTEGNGWEYTWLVPQDPEQLISLMGGDKPFVAKLDSLFT
                     VTGSMGDAPPDISGMVGMYAQGNEPNHHVPYLYAFAGIQWKTAERIAHIARKFYTAGK
                     DGLCGNDDAGQMSAWYVMSALGFYPVNPCNGIFVFGTPLVRKAMVEVGEGKLFAIETE
                     NFGLKNIYIQRVTLNGAPYSKSYIDYHTIMTGGTLRLYMGDRPNKEFGAAPADRPVSS
                     PS"
BASE COUNT          614 a          755 c          678 g          545 t
ORIGIN      
        1 tcgtcggcga ttatctgaac gaacatgcgt ccatcgttgc cgggctctgg ttgctttgtc
       61 ttacgtttca actggtgcgt ctgacgggag gtctttacca aatgcgccgg tttcgccgga
      121 aaggcatcca tcctccggga atcgaatggg tcgggcggct ggcgacattg tgcagggtgg
      181 ctggcgtccg gaaaaaagtg ctcctgctgc aatcggaatt ggtcagcatg ccggtgacct
      241 tcggctttct gaaaccgacc attcttattc cgtttggaat gatagcgggt ctgcctgccg
      301 accagctgga gacgatcctg ctgcacgagc tggcgcatat ccgcaggaac gactatctgt
      361 ccaacctgtt actctatata acagagtccg tctttttctt caacccggcc tttcgttgga
      421 tggcatctcg tatccgccag gagcgggaag cctgctgtga tgatatcgtc ctcgcggata
      481 cgcccaaccg gaacagctat ttcgacgcgc tgatcgcttt ccgggtacag gcaatggcac
      541 aagaaggcta cccgctgcag ctgggcatcg gcaagacaga tctgttatgg cgtattcgcc
      601 gtatgctcga taaagaaaat aaaaaattac atgttatgga aaaagctctt ttatccgtag
      661 gattgatgat cgtcctggcc atcggcctgg tcagcatgaa cgaatggcag gctccgccgc
      721 cggcagtgac ctccgctccc gccgaagtat cgtccgatac tgcaccggca atcaaagttc
      781 cttacccgag tatcagcaag agcagcgata tcgagggagc caggaagaca tgcaagatct
      841 cggctacgga tgccgagggc aatacgttcc agctgaccaa aaccaacggc gagatcactg
      901 aaatgatcat caacgggaat gtgctgtcgc ccgccgatta tgaccggtat gaatatattt
      961 tcgacaagat agaaagacac cgcgaggcgc gcgagaatgc gcagtccgat gaagcaaccg
     1021 aggcgaccga acaggtagag gccaccgaag ccacggaagc taccgaagcc acggaagcca
     1081 ccgagaccac tgaagctacc gaggccacgg aagctcccga aaccaccgaa gccaccgaag
     1141 ccaccgaagt caccgaggcg cccgctcccg catccaaccg ctggatccat tgcatcatca
     1201 aagacctgac ccgcaacggc atcatcgcaa acgaagatcc cctgtccttc acgctggaca
     1261 aagaccatct gatcgtcaac ggcgtccgcc agcccgacga agtctatcgt ctgttcaaag
     1321 aaaaatacat caataaccca aaagatcatt tcgactattc ccgcgacggc ggctcgaccc
     1381 acgtcgaagt ctgggtggag taagatcagg agggcgatga caccggccgg tccgcgggag
     1441 cggcgccgaa ttccttattc ggtctgtcgc ccatatacaa ccgcagcgtg ccgccggtca
     1501 tgatcgtatg ataatctata taagacttac tatagggcgc cccattcagc gtcacgcgct
     1561 gaatataaat attcttcagc ccgaaatttt cggtctctat cgcaaacagc ttcccttcgc
     1621 cgacctccac catcgccttt cgtactaacg gcgtcccgaa aacgaagatt ccgttacacg
     1681 gatttaccgg gtagaacccc agcgccgaca tgacatacca ggcgctcatc tgcccggcgt
     1741 cgtcattgcc gcaaagacca tctttccctg ctgtatagaa cttacgcgcg atatgggcga
     1801 tccgctccgc agtcttccac tgaatgccgg cgaaagcgta gagatatgga acatgatgat
     1861 tcggctcgtt gccctgcgca tacataccga ccatacccga gatatccggc ggagcgtcac
     1921 ccatcgagcc tgtgacggtg aaaaggctgt ccagcttggc gacgaaaggc ttgtcgccgc
     1981 ccatcaggct gatcaactgt tccgggtcct gcggcaccag ccaggtatac tcccagccat
     2041 tgccttcggt ataattcttt gtcaggaacg ggctgtacgg ggtaatgaat tttccgtccg
     2101 ccagcacggg acgcatgaac cgtgtcgacg gatcgaaata tcgggtgaac agctgcgacc
     2161 ttcgtgtata tgtcgctgca tcttccgtgc gtcccatttg ggaggccata cgcgcgatac
     2221 accagtcatc gatggcgtat tccatcccct tcgatatcgc ctctacctcc ttgtcggcgg
     2281 gaataaagcc gtcctgtttg agatacttca ggccatagtc gtcgcgggta gaagtagcct
     2341 tcatcgctgt gagcgccagc cccgcgtcaa aaccatgaaa acctttcagc caggcgtcgg
     2401 cgatgacggg caccgcgctg tatccgacca tacagtccgt ctcacgaccc tgcaaatgcc
     2461 aaacgggcag cttaccctgc tgctgataga tagcgagcat ggtattgacg aagtcgttga
     2521 ctctttccgg ctggatgatg gtcatcagcg gatgaaaggt ccggtatgta tcccagagcg
     2581 aaaagatcgt at
//
LOCUS       DASYSV010000051         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1807203, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000051 DASYSV010000000
VERSION     DASYSV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807203"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..>2338)
                     /locus_tag="VGQ51_01570"
     CDS             complement(28..>2338)
                     /locus_tag="VGQ51_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035271.1"
                     /translation="FGLALGMACSLLILLWIRDEKSMDGYHVNRDRIYNVYERQYYDA
                     KVSSGFYTPGVLPDQMKKDLPEVEMSSGFTWQNEYTFQVNDKILKEKGRFAGADFFRM
                     FTFPLLEGNPSAALSNPVSIAISRKMANDFFGSPGAAIGQAIRFENRKVFKITAVFEN
                     VPQNTSNKFDYLINWTAFLDENSWAKDWGNNGPQTILLLRKDADPAAFRRKITHYLAT
                     YNKEMSKSFRIELGIQRYPDGYLYSNFDPTGQIDGGRIEYVRLFSVVAIFILLIACIN
                     FMNLTTARSVKRAREIGIRKVVGAVRPALIRQFIGEAVLISCFSVLLALGMVLLLLPG
                     FDHMTEKTISFPFSDGRFWLSLAGLTLVTGIVSGSYPALFLSAFQPIRVLKGSMKFSP
                     RATWFRKGLVVFQFVLSIALIIGTIVVSGQVNYIQSVNLGFDRDNVVYLPLEGDMPDK
                     YKTFKEQALLLPGVTGITKVSQEPTDIENGTGGIDWDGKDPNNLIMFTNVSVGYDFVK
                     TMRLQLVKGREYSSAYGTDTAGYMLNEEALRRIGYKDPIGRRLTMWGKKGTIIGIVKN
                     FHFNSLHDPMPPLIIRYGEDGKDNNYGSIVVRIKGGQMRQTLSQLETLSHQLNPKFPF
                     TYRFADDEFRNLYKSEALIHRLSNCFAGLAICICCMGLLGLAMFTAEQRTKEFGIRKV
                     LGAKAGTLFSLLSRDFLSLVLIAFLLAMPLAGWAMHHWLQNFAYHIDLEWWVFLLAGM
                     MALMIALLTVSFQAFRVAVANPVKSLRTE"
BASE COUNT          564 a          643 c          613 g          518 t
ORIGIN      
        1 attatctgaa aatgcaacct gaatcgtcta ttccgtccga aggctcttta ccggattagc
       61 caccgccacc ctgaacgcct ggaagctgac cgtgagcagg gcgatcatca gcgccatcat
      121 gcccgccagc agaaagaccc accactcaag atcgatatga taggcgaagt tttgcaacca
      181 gtgatgcatg gcccatcctg ccagcggcat cgccagcagg aaggcgatca gtaccagcga
      241 gaggaaatcc cgcgacaaca aggaaaaaag cgttcccgct ttggcgccca gcaccttgcg
      301 aatgccgaat tcctttgtcc gttgttcagc ggtaaacatc gcgaggccca gaaggcccat
      361 acaacagatg cagatcgcca aaccggcaaa acaattggaa agacgatgga tgagcgcttc
      421 gctcttgtac agattgcgga attcatcgtc ggcgaaccgg taggtgaacg ggaatttcgg
      481 gttcagctgg tgtgacaggg tttccagttg gctcagcgtc tgccgcatct gaccgccctt
      541 tatccgcacc acgatgctgc cataattgtt atccttgccg tcctcgccat aacggatgat
      601 cagcggcggc atcgggtcgt gcagcgaatt gaaatgaaag ttctttacaa tgccgatgat
      661 cgtgcccttc tttccccaca tggtcagtcg acggccgata ggatccttat aaccgatgcg
      721 acgcagcgcc tcttcgttga gcatatagcc cgcggtatcc gtcccgtagg cggaggaata
      781 ttccctgccc ttcaccagtt gcagccgcat ggtctttacg aaatcatatc ctaccgatac
      841 attcgtgaac atgatgagat tgttcgggtc cttcccgtcc cagtcgatgc ctcccgtgcc
      901 gttctcgata tccgtgggct cctgcgacac tttggtaata cccgtcactc ccggcagcaa
      961 cagcgcctgt tccttgaaag ttttatattt atccggcatg tctccttcga ggggcagata
     1021 gacgacatta tcccgatcga atcccaggtt caccgactga atataattca cctgcccgga
     1081 cacgacgatg gtcccgatga tgagggcgat ggaaaggacg aattggaata cgacaagccc
     1141 tttgcgaaac cacgtggcac ggggactgaa tttcatcgat cctttgagca cgcgtatcgg
     1201 ctggaaggcg gataggaata gtgccggata gctgccggaa acgatgccgg taacgagcgt
     1261 gagccccgcc aggcttagcc aaaacctgcc gtcggagaag gggaatgaaa tggtcttctc
     1321 ggtcatgtga tcaaaacccg gtagcagcaa caggaccatg cccaatgcca gcaggacgga
     1381 aaaacagctg ataagcacgg cttcgccgat aaactgacgg atgagcgctg gacgaaccgc
     1441 gccaaccacc ttacggatac cgatctccct ggcacgtttg accgaccgtg cagtggtcag
     1501 gttcataaaa ttgatacagg cgatgagcag gataaagatc gccactacgc tgaacaggcg
     1561 tacatattcg atgcggccgc catcgatctg gccggtgggg tcgaagttgg aataaagata
     1621 gccatccgga tagcgctgga tgcccagctc gatacggaaa gacttgctca tctccttatt
     1681 ataagtcgcg agataatgag tgatcttccg tctgaatgcg gccggatccg catccttacg
     1741 cagcagcaaa atcgtttgcg gaccgttgtt accccaatct ttggcccagg agttttcgtc
     1801 gagaaaggcg gtccaattga tcaggtaatc gaatttgttg ctggtgtttt ggggcacatt
     1861 ttcgaatact gcggtgattt tgaacacctt gcgattctcg aaccgaatgg cttggccaat
     1921 tgccgccccc ggacttccga aaaaatcgtt tgccattttc cgcgagatgg cgatgctgac
     1981 cgggttggac agcgccgccg aaggattgcc ctccagcagc ggaaacgtga acatgcggaa
     2041 aaaatcggcg ccggcaaaac ggcccttttc cttgagtatc ttatcattta cctggaaagt
     2101 gtattcgttc tgccaggtga atcccgagga catctccacc tcgggcaggt cctttttcat
     2161 ctggtcgggc aatacccccg gcgtatagaa tccggaggaa accttcgcgt cataatattg
     2221 gcgctcataa acgttgtata tcctgtcgcg attgacgtgg tacccatcca tgcttttttc
     2281 atcccggatc caaagcagga tcaacagact gcaggccatg cccagggcca ggccgaag
//
LOCUS       DASYSV010000052         8995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2445692, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000052 DASYSV010000000
VERSION     DASYSV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445692"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VGQ51_01575"
     CDS             complement(<1..341)
                     /locus_tag="VGQ51_01575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV9035272.1"
                     /translation="MKFLIIRFSSIGDIVLTTPVVRCLKKQLLTAEVHYLTKRSFRPI
                     LAANPYVDKIHVLEDDLETVIETLRQEDFDYVIDLHHNLRTLKVKRGLGKKAVSFDKL
                     NVKKWLYTNFK"
     gene            complement(343..1578)
                     /locus_tag="VGQ51_01580"
     CDS             complement(343..1578)
                     /locus_tag="VGQ51_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9035273.1"
                     /translation="MEKKLHPLFNLAVIVGALGYFVDVYDLLLFSIIRVPSLKSLGIA
                     ADDGRGLNILTIQMYGLLIGGIIWGVLGDKRGRLRVLFASIILYSLGSIANGMVHTVG
                     QYALARFVTGLGLAGELGAGITLVSELMPKEKRGIATAWVAGIGLSGAVFAYLIKYWF
                     VGTDGSGWRTCYYIGGGLGFLLLLLRVGVLESGMYKHMAVSNVSRGNIFMLFTNGQRF
                     KKYITAILIALPNWYVIGILITFCDKFGADMKVHGAIDPGKGIMVAYAAISIGDVLIG
                     FISQWMKSRKKSLWLFNIITVFGVIAYFNLQGASSTALYWVCVVLGFGTGFWAMFVTM
                     AAEQFGTNIRATVATTVPNMARGSLAVVVVPIFKGLLPSLGHYGAGWVTGIIVFTISF
                     IALALTPETFGKDLNYIEA"
     gene            complement(1582..2283)
                     /locus_tag="VGQ51_01585"
     CDS             complement(1582..2283)
                     /locus_tag="VGQ51_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEV9035274.1"
                     /translation="MLEFLTITWKDLLNPDFYINNGGLWVMLFIVFAETGLMFGFFLP
                     GDSLLFVAGIYSEKLVGSLISGGTGTDIFDLIVLWLLVSVAGIIGNAVGYWIGNKSGP
                     FLFHRKDTLFFKKKYLFQAKAFYDKHGGEAIVFARFLPIVRTFAPIIAGIVAMNKRKF
                     HLYNVVGCVAWVFAMLFAGHYLDKLFLTKFGFDLKKHLEVIVIGIVVITTAPVIYKLF
                     FGRKGTAPAEDPYQH"
     gene            complement(2319..3554)
                     /locus_tag="VGQ51_01590"
     CDS             complement(2319..3554)
                     /locus_tag="VGQ51_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEV9035275.1"
                     /translation="MPKISLTGWIMIAMVAGVLLGLWVHYSGNPTQIDTFSSAMGVIT
                     DVFLRMIKMIIAPLVFSTLVVGIAKLGDIKAVGRIGGKALLWFLSASFLSLLLGLIIM
                     NSLSLGSELNLPVPASGAVSGIETTSVTFKSFITNIFPRSIFEALSGNEVLQIVVFAV
                     FFGIATASIGEKGKVVIKALDAVSLIILRVTGYVMYFAPVAALAAISAVMAAKGPELL
                     VTYGKLIISFYLAILGLWLILLLVASLIIGRRVIHLAFAIKDMILLAFSTASSEAAFP
                     RLIVELENFGCPPKIVSFVLPLGYSFNLDGSMLYMTFASLFIAQAYGVPLTLGQELSM
                     LLVLMLTSKGVAGVPRAALVVIAGTLSLYNIPQQGLLLLLGIDQILDMARSATNVLGN
                     SLATVVVSKWEGELEEAAI"
     gene            3743..5239
                     /locus_tag="VGQ51_01595"
     CDS             3743..5239
                     /locus_tag="VGQ51_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV9035276.1"
                     /translation="MSLFRKKTLAVLLAQADESEKGLKRTLGVWSLIALGIGAIIGAG
                     LFVRTAAAAGEHAGPAVTISFIIAAVGCCFAGLCYAEFASIIPIAGSAYTYAYATMGE
                     LVAWIIGWALILEYALGAATVAISWSEYLNNLLYNLFQYKIPFEWCHSPMQHLKDAAG
                     VMHSGIINLPALFILMCLTLLLIKGMQESAAVNGIIVFLKVSIVVIFIIIGWAYINPA
                     NHHPYIIPADAAPALQADGKYYSYLPFLNHGWGGVLRGAAVVFFAFIGFDAVSTAAQE
                     AKNPKKDMPMGILGSLAICTLLYILFSYVLTGVAPFTDFIKAGKEASVAYAIQAYMPG
                     HQWLGTLVTIAILLGFSSVILVMLMGQSRVFYTMSTDGLLPKVFSDLHPKFRTPYKSN
                     MLLFIFVGLFAGFLPESVAGDLTSIGTLLAFVLVCAGIIILRRRDPNLHRPFKTPLVP
                     LVPILGIIVCAAMIFSLWGTTLLSAFVWMIIGLLIYFGYSKNNSKLKV"
     gene            5299..6021
                     /locus_tag="VGQ51_01600"
     CDS             5299..6021
                     /locus_tag="VGQ51_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV9035277.1"
                     /translation="MGRIFEVRKSTMFARWDRMAKQFSRIGKEIAIAVKTGGPDPATN
                     PGLRRCMQNAKSVNMPKDRVEAAIKRAQGKEMENYEEILYEGYGPHGVAILVETATDN
                     HVRTVANVKSHFNKGNGTLGNSGSVSFQFKKMGVFKLKPEGISADDLELELIDFGLEE
                     MGEGTGEHGEPVLVLRAEFNDFGRMQKALEEKHITPISAETEWIPSSTVELSEDMAQD
                     VLKLVDRLEQDEDVQKVFYNLK"
     gene            complement(6036..8429)
                     /locus_tag="VGQ51_01605"
     CDS             complement(6036..8429)
                     /locus_tag="VGQ51_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV9035278.1"
                     /translation="MENFAPGEVLDPEKLSFDRFRDEVLNDYRLACESREASLLGRKE
                     VLTGKAKFGIFGDGKEVPQVAMAKFFKPGDFYAGYYRDQTFAFATGVATIEEFFSQLY
                     ADPDVRHDPHSAGRQMNSHFASAFIDTDGSPLDLVNRKNLTAGLAPTAAQMPKALGLA
                     CASKAFRDVRLLDHLSNLSNNGNEVCFCTIGDASTSEGHFWETLNAAGVLQVPLAIFV
                     WDDGYGISVPRQYQTTKGSISEVLEGFQKREGTNGIDIYKVKAWDYAGMCEVFEAGLR
                     NVRQTHTPALFHVEEVTQPQGHSTSGSHERYKTPERLEWEREWDGVKKMKEWIVENAL
                     ASEDELGDIEVEAKELTRNSRNLAWEKYQAPIRVQVSRSVELIREMAHQLPEQAETLQ
                     KLASALALNREPGRKEVLQTLHTAMGVAGKEPASRNASDYYQQLRVENARLYNTHLYH
                     EGDRRILHVPEIKAEYSFNSPVLNGYEVLNRYFDLLFESNPKVLAFGEDLGKIGDVNQ
                     GFAGLQAKHGEERIFDTGIRELTIMGQGIGMALRGLRPIAEIQYIDYLLYGLQPLSDD
                     LASLQYRTVGRQTAPLIVRTRGHRLEGIWHSGSPMGMILHSLRGLLVCVPRNMVQAAG
                     MYNTLLEGVDPALMIECLNGYRLKEKLPSNLLEFRVPLGVPEVLTEGDDITIVSYGST
                     LRIVQEAAEILQARGISCEVVDVQTLLPFDVHHNILESLKKTSRLLIVDEDVPGGAAG
                     FIFNKVMEEQGGYRWLDTAPRTLTGQAHRPAYSSDGDYFSKPNAEDIIDTVMEMMAE"
     gene            8551..>8995
                     /locus_tag="VGQ51_01610"
     CDS             8551..>8995
                     /locus_tag="VGQ51_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEV9035279.1"
                     /translation="MKKTAIAAFLLFTGLYAGAQTAGATDATTPHLQPNTGATALPRP
                     ADVLQLKENLHNFGKIPQGRPATYTFEIVNTGSVPLRLDNVQASCGCTTPVWSRDAIE
                     PGATGKILVGYNAYSEGSFTKTVTIIYNTSRTKTLTITGEVYKAPP"
BASE COUNT         2161 a         2566 c         2232 g         2036 t
ORIGIN      
        1 atcttgaaat tggtgtacaa ccatttcttt acattcaatt tatcgaaaga cacggccttc
       61 tttcccagcc ctcgtttcac cttcagcgtg cgaagattat gatggaggtc gatgacgtag
      121 tcgaagtcct cctgtcgcag cgtttctatc acggtctcca ggtcatcttc cagcacatgg
      181 atcttatcta catagggatt ggctgcgagg atgggccgga aggatctttt agtcaaataa
      241 tggacctccg ccgtgagtaa ctgctttttc aaacaacgga ccaccggggt agtgaggacg
      301 atatccccga tggaactgaa ccgtataata agaaacttca tcttatgctt ctatataatt
      361 cagatctttt ccaaaagtct ccggcgtcag cgcgagcgca ataaacgaga tcgtaaacac
      421 gatgatgccc gtcacccagc ctgccccata atgccccagc gatggcaaaa gccctttgaa
      481 tatggggacc accactacag ccagcgaccc gcgggccata ttcggaacgg tcgtcgccac
      541 cgtcgcccgg atattcgtcc cgaactgctc ggcggccatc gtcacgaaca tcgcccagaa
      601 accagtcccg aatcccaata ccacgcaaac ccaatacaag gcggtggacg acgccccctg
      661 caaattaaaa taagcgatca ctccgaatac cgtaataata ttgaagagcc acaacgattt
      721 tttccggctc ttcatccatt gactgataaa cccgatcagc acatctccta tcgagatggc
      781 ggcataggct accatgatcc ccttgcccgg atcgatcgct ccatgcacct tcatgtccgc
      841 tccgaacttg tcgcaaaagg tgatcaggat cccgatcaca taccaattcg gcaacgctat
      901 cagtatagct gtaatatatt tcttaaaccg ctgaccattc gtgaagagca tgaatatatt
      961 tcccctgctt acgttcgata ccgccatgtg cttatacatc ccggactcca gcacaccaac
     1021 ccgtaataac aagagcaaaa atccaagccc gccgccaata taatagcagg tgcgccaacc
     1081 actgccatcc gtacctacaa accaatattt gatcaggtag gcaaagacgg ctccgctgag
     1141 tccgataccg gctacccaag cggtggcgat gcctctcttc tctttcggca tcagctcgct
     1201 caccagcgta ataccggcgc ccagttctcc agccagaccc aatcccgtaa caaaacgagc
     1261 cagcgcatat tgtccaacgg tatgaaccat cccattggcg atgctcccca atgaatacaa
     1321 aatgatcgat gcaaacagga cccgcaaacg gcctctttta tctcccagca caccccagat
     1381 gataccgccg atgagcagtc cgtacatttg gatggtcaat atgttcaggc cccgcccgtc
     1441 atccgccgct atgccaaggc tcttcaatga aggcacgcgg ataatactga acaacaaaag
     1501 atcatataca tctacaaaat atcccaacgc cccgacaatg actgcaaggt tgaaaagcgg
     1561 gtgtaatttt ttttccatgg cttagtgttg atacgggtcc tcggcggggg cggtcccctt
     1621 ccggccgaaa aacagcttgt aaataacagg cgctgtagtg atgacgacga tcccgatcac
     1681 gatcacctcg agatgttttt taagatcgaa accgaacttc gtcaggaaca gtttgtcaag
     1741 gtaatgcccc gcaaacagca tggcgaacac ccaggcgaca caccccacca cattatagag
     1801 atggaatttc cgcttgttca tggccacgat acccgcgatt ataggagcga aggtccggac
     1861 gatgggcagg aaacgtgcga atacgatggc ttcgccgcca tgtttgtcat agaaggcctt
     1921 ggcctggaaa agatacttct ttttgaaaaa aagcgtgtcc ttccgatgaa aaagaaatgg
     1981 cccgctctta ttcccgatcc aatagccgac ggcattcccg ataatgcccg cgaccgagac
     2041 cagcaaccat aatactatga gatcgaatat gtcggtaccc gttccgccgc tgatcaggct
     2101 ccccaccagc ttttcgctat agatgccggc gacgaacagc aggctgtccc ccggcaaaaa
     2161 aaagccgaac atgagaccgg tctcagcaaa gacgataaac agcattaccc aaagtccgcc
     2221 gttattgatg tagaagtcag ggtttaatag atctttccag gtaatagtca agaattcaag
     2281 catacattca ttttatgttt atcgccgccg atggcggctt agatggcggc ttcttccagc
     2341 tcgccttccc atttgctgac caccaccgta gccaggctgt tgccgagaac attcgtggcg
     2401 ctgcgtgcca tatccaatat ctggtcgatg cccagcaaca gaagtaaacc ctgctgtgga
     2461 atattatata aagagagcgt acccgcaatc actacaagcg ccgccctggg cactccggct
     2521 acgcctttgc tcgtcaacat caacaccagc aacatcgaca gctcctgccc cagggtcagc
     2581 ggcacgccgt atgcctgcgc gataaacaga gaagcaaagg tcatataaag catgctgccg
     2641 tccagattga aagaatagcc gagtggaaga acgaaactga cgatcttggg tgggcaaccg
     2701 aaattctcca gttcgacgat cagccgggga aacgcggcct cggaactggc cgtactgaat
     2761 gccagcaaga tcatatcctt gatcgcgaat gccaggtgga tcaccctccg gccgatgatc
     2821 agggaagcca ccagcagcag gatcaaccat aaccccagta tggccaggta aaagctgatg
     2881 atcagtttgc cgtaggtgac cagcaactcg gggccctttg ccgccatgac agccgaaatg
     2941 gcagccagcg ccgccaccgg ggcaaaatac atcacgtagc ccgttacgcg aaggatgatc
     3001 agcgaaacgg catccagggc tttgatcacg accttcccct tttcacctat cgatgcggtg
     3061 gcgatcccga aaaaaactgc aaaaaccacg atctgtaaga cctcatttcc cgacagcgcc
     3121 tcgaaaatac tgcgtggaaa gatattggtg ataaaacttt tgaaggtgac cgatgtggtt
     3181 tcgatcccgc tgacggcccc cgaggccggc acgggcagat tgagctcgct gcccagcgaa
     3241 agactgttca tgatgatcag gccgagcaac agggagagaa aagaggcgct gagaaaccag
     3301 aggagggcct tgccgccgat gcgtccgacg gccttgatgt ctcccagttt ggctatgccc
     3361 actacaagag tggaaaagac cagtggagca atgatcattt tgatcatccg caaaaagaca
     3421 tcggtgatga ctcccatggc cgaagaaaag gtatctatct gggtggggtt gccgctataa
     3481 tgcacccaaa gtcccagaag gacgccggcc accatggcga tcataatcca tcctgtaaga
     3541 ctgatcttgg gcataggtct aagcacgcgg gccggccccc gctccgcctt ttagctaatg
     3601 tcgggccttc ggccaatggg ccaccggccc attctggcct gcaaagaatc gaaaaatatt
     3661 gatttggcaa aaatttgttt attttcggcc ctagtcgtca cacatcaaag cattcaccaa
     3721 ttttacctaa acgatcttgc acatgagttt attcagaaaa aagacattgg ctgtccttct
     3781 ggcgcaggcc gatgagtcgg aaaaaggcct gaaaaggacc ctgggcgtat ggagcctgat
     3841 cgccctgggc atcggagcca tcatcggggc cggtctgttc gtccggacag ccgccgcggc
     3901 aggggagcat gcgggacccg ccgttaccat ctcctttatt attgccgccg tcggttgctg
     3961 ttttgcggga ctttgttacg ccgagttcgc ctccatcata cctatcgcag gtagcgctta
     4021 tacctacgct tatgccacca tgggcgaact ggtcgcctgg atcatcggct gggcgctgat
     4081 cctcgaatat gcgctcggcg ccgctactgt agccatatcc tggagcgaat acctcaataa
     4141 tctgttatat aacctcttcc aatataagat accattcgaa tggtgtcatt ccccgatgca
     4201 gcatttgaaa gacgccgccg gcgtcatgca ctcgggcatc atcaacctcc ccgccctatt
     4261 catcctgatg tgtcttaccc tgctgctgat caagggcatg caggaatcgg ccgccgtcaa
     4321 cgggatcatc gtttttctca aggtgtccat cgtcgtgatc ttcattatca tcggctgggc
     4381 ctatatcaat ccggcaaatc atcaccccta tatcataccg gccgatgccg caccggcatt
     4441 acaggccgat ggcaaatatt actcctacct gcccttcctc aaccacggat gggggggtgt
     4501 tctgcgaggg gcggccgtcg tattctttgc cttcatcggg tttgacgccg taagtacggc
     4561 cgcacaggag gcaaaaaatc ccaaaaagga catgccgatg ggtatcctcg gatcgctggc
     4621 gatctgcacc ttattgtata tcctgttctc ctatgtgctt accggcgtgg cccctttcac
     4681 ggatttcatc aaggctggaa aggaagcttc ggtcgcctat gcgatacagg cctatatgcc
     4741 gggtcaccaa tggttaggta cacttgtaac gatcgccatc ctgctgggtt tctcctcggt
     4801 gatcctggtg atgctcatgg gccaaagccg cgttttctat accatgtcta cggatggcct
     4861 gctgcccaag gtcttttccg acctgcatcc taaattcagg acgccctata agagcaatat
     4921 gctgctgttc atcttcgtag gccttttcgc cggtttcctc ccggaaagcg tggccgggga
     4981 ccttacctct atcgggacgc tgctggcctt cgtgctggtt tgcgcgggta tcatcatcct
     5041 gcggagaaga gacccgaatc tccatcgtcc cttcaagacc ccgctggtac cgctggtccc
     5101 catattgggt ataatcgttt gtgcggccat gatcttcagc ctttggggta ctacgctgct
     5161 cagcgccttt gtttggatga tcatcggtct gctcatttat tttggttaca gtaaaaataa
     5221 cagcaagcta aaagtctgat aaattactaa atttgccccc cggcgccgcg aagcggcgcc
     5281 gggcctaaat attttcgcat gggtcgcatt tttgaagtac gcaaatccac gatgttcgcc
     5341 cgctgggacc ggatggccaa gcagttttcg cgtataggta aggaaatcgc cattgccgtc
     5401 aagaccggcg gtcccgatcc tgctacgaat ccaggcctgc ggcggtgtat gcagaacgcc
     5461 aaaagtgtca acatgcccaa ggacagggtc gaagccgcca tcaagcgggc ccagggcaag
     5521 gaaatggaga attacgagga gatcctgtat gaagggtatg gtccccacgg cgttgcgatc
     5581 ctcgtggaaa cggccaccga caaccacgtc cggacggtcg ccaatgtcaa atcgcatttc
     5641 aacaaaggca acggcacact cggcaacagc ggcagcgtga gcttccagtt caaaaaaatg
     5701 ggcgtcttca aactaaaacc cgaaggcatc agcgcagatg atctcgagct cgagctgatc
     5761 gatttcggtc tggaggaaat gggcgaaggg accggcgaac acggggaacc cgtacttgta
     5821 cttcgggcgg agttcaacga tttcggaaga atgcagaagg cgctggaaga aaaacacatc
     5881 accccgatca gcgcagaaac ggaatggatc ccgtcttcta cagtcgagct ttcggaggac
     5941 atggcgcagg atgtgctcaa acttgtcgac cggctcgaac aggacgaaga cgtacaaaag
     6001 gtattttaca atttgaaata ggcaagtggg cttttttatt ccgccatcat ctccattacc
     6061 gtatcgatga tatcttccgc attcggcttg ctgaaataat cgccgtccga agaataggca
     6121 ggccggtgtg cctgacccgt cagcgtcctc ggggccgtat ccagccagcg gtaccctccc
     6181 tgctcttcca tcactttatt aaagatgaag cctgcggcgc cgcccgggac atcttcatcc
     6241 acaattaaca gtcggctcgt ttttttcagg gattccagga tattatgatg cacgtcgaaa
     6301 ggcagcagcg tctgcacatc cacgacctcg cagctgatgc cgcgagcctg gaggatctcg
     6361 gcggcctcct gcacgattcg aagcgtcgaa ccataggaga cgatagtaat atcatcccct
     6421 tccgtcagta cttccgggac tcccagcggg accctgaatt ccagcagatt cgacggtaat
     6481 ttctccttta gccggtagcc gttgagacat tcgatcatca gcgcgggatc gacgccctcc
     6541 aataaggtgt tgtacattcc cgcggcctgc accatattgc ggggtacaca caccaacagg
     6601 ccacgcagcg aatgcaggat catccccatg ggagagccgc tgtgccagat accttccagg
     6661 cgatgaccgc gcgtacgcac gatcagcggt gctgtctgtc ggccgacggt ccggtattgg
     6721 agcgaggcaa gatcatcgct cagcggctgg agaccatata aaagataatc gatgtactgg
     6781 atctctgcga tgggtcgtaa accacgaagc gccataccga tgccttgtcc cataatcgta
     6841 agttcgcgga taccggtatc gaagatgcgt tcttccccat gttttgcctg gagacctgca
     6901 aatccctgat tcacgtcccc gatcttaccg aggtcttccc cgaaggccag caccttcgga
     6961 ttgctctcga acaacaggtc aaaataccgg ttcaggacct cataaccatt cagcaccggc
     7021 gagttgaaag agtactccgc ttttatctcg ggcacgtgca gtatccggcg gtcgccttca
     7081 tgataaaggt gtgtattata tagccgggcg ttctcgacac gcagctgctg gtaatagtcg
     7141 ctggcattcc gggaggcagg ttcctttccc gcaacaccca tggcggtgtg cagggtttgc
     7201 aggacttctt tgcgacccgg ctcgcgattc aaagccagcg cggaagccag cttctgtaat
     7261 gtctcggcct gttcaggtaa ttggtgggcc atttcacgga tgagctctac actacggctg
     7321 acctgaaccc ttatcggagc ctggtatttt tcccaggcaa gattgcgact gttccgggta
     7381 agctccttgg cttcgacctc gatatccccc aattcgtcct cgctggccag ggcgttttcg
     7441 acgatccatt ccttcatttt cttgacgccg tcccactccc tctcccattc caggcgttcg
     7501 ggggttttat atctttcatg gctgcccgag gtggaatgcc cctgcggctg cgtaacttct
     7561 tcgacatgga acagggccgg ggtatgtgtt tgacggacgt tccgcagccc tgcctcgaag
     7621 acctcgcaca tgcctgcata atcccaggct ttcactttat aaatgtctat accgttggtg
     7681 ccttctcttt tctgaaaacc ctccagaacc tccgaaatgg accccttcgt ggtctggtat
     7741 tgtctcggga ccgagatccc atacccgtcg tcccacacga aaatggccag ggggacttgt
     7801 agcacgcctg ccgcattcag agtttcccag aagtgccctt cactggtgga agcatcgccg
     7861 atcgtgcaga aacaaacttc attgccgttg ttggataaat tgctcagatg gtccaggagt
     7921 ctcacgtccc ggaaagcctt ggatgcgcag gccaggccca gcgccttggg catctgggcg
     7981 gccgtaggtg ccagtcccgc cgtcaggttc ttgcggttca ccaggtcaag cggcgacccg
     8041 tccgtatcga taaaggcgct tgcgaaatgc gagttcatct gccggccggc gctatgcggg
     8101 tcgtgacgga catccggatc ggcatagagt tgcgaaaaga attcctcgat ggtggcgact
     8161 cctgtggcaa aagcaaaggt ctgatcacga taatatcctg catagaaatc acccggtttg
     8221 aagaatttgg ccatcgctac ctggggcacc tcctttccgt cgccgaagat gccgaatttg
     8281 gctttccccg tcagcacctc tttgcggcca aggaggctgg cttcccggct ttcgcaggcc
     8341 agccggtaat cgttcaatac ttcatccctg aatctgtcga aggatagctt ttcgggatcg
     8401 aggacttcgc cgggcgcaaa attttccatc ggaataattt accaaaatta cctgtatctg
     8461 attgattgtt aaaaaaataa aattaggggg tttttattgg taaagctgac cgccaatgct
     8521 taattttagg tgcttaactc gggccaaact atgaaaaaaa cagcaattgc cgcttttctt
     8581 ttattcaccg gattgtacgc tggtgcacag acggcgggtg ccaccgatgc taccactccc
     8641 cacctccaac ccaacactgg ggcaacggcg ctgccccgcc ccgccgatgt ccttcagttg
     8701 aaggagaacc tccacaactt cggtaagatc ccccagggac gtcccgcaac ttatactttc
     8761 gagatcgtca ataccggtag tgtcccgttg cggctggata atgtgcaggc ctcctgcggc
     8821 tgtaccactc ccgtctggag ccgcgatgcc atcgaacccg gcgctaccgg aaagatcctg
     8881 gtcggttata atgcgtatag tgaaggttct ttcacgaaga ccgtcaccat catttataac
     8941 accagccgga cgaaaacgct tactattacc ggcgaggtat ataaggcgcc gccga
//
LOCUS       DASYSV010000053         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1166263, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000053 DASYSV010000000
VERSION     DASYSV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166263"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1809
                     /locus_tag="VGQ51_01615"
     CDS             223..1809
                     /locus_tag="VGQ51_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035280.1"
                     /translation="MNYFDFHAHILLKQLFEDTPDVNQTVSPGDVKLIPEDCSDLPFI
                     IETQIHQTQLLSMHSPVLIGAALYGMESFLAAVVDPLRSFLKDASQAKLSHNVLTHIA
                     APDYKTFSTFTKANTLDRYLGAPNLFNILGKANATAPLDPKKVNIFFVVEGCHSLVDS
                     KNRVNLATGDGYDPKEILANLDILLRQVTILAVNLTHLQQSNLCNHAFGMQIADPGNF
                     FPAGNGLTDAGRTVVRGLFDRGICVDVKHMSYLSRLQLRNEIDAGKYTNPQPLLCTHA
                     GFTGTSIAEWPTFIRKQLPVNPGTMYLEIAKNIQAYTNRTRPGAPAFNLSTINLFDDE
                     IVWIVKHGGMIGLSMDRRILGFVSTFDVKPTGIGAGSEFTVDKEYMSMAEWKVLGINK
                     IGDAVDEDHCELLSEVGEFMSAATPIPMRDAFFYAHVMVHIKHFLQVCIDNGIPLDTA
                     RKHLCIGSDFDGVINPFLNSRTCLEMPALRSFLQSRFKIFLKGLTDSVQWADQLDIGG
                     FLEDLFFNNGFTFVTGHLAG"
     gene            1861..>2763
                     /locus_tag="VGQ51_01620"
     CDS             1861..>2763
                     /locus_tag="VGQ51_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035281.1"
                     /translation="MRPPHRLFSPALLCGAILFSIASFAQTTPAQTTSTQTTPDTIHV
                     AGKVLVRNHGLYMYDSVEWQAMQWQKQHRKETKPSRPAFLSTDSAATPGQTLLVPAPL
                     ILQQNTAHPLNNCGVSASFTPGDDSVLTTMTGPVTFTSTSTNATSLTWLDVGIPISNG
                     NTFTWGENQPGLYDIMLVAQNGACTDTAIAYYYIKGVQPANRRNLWAYYGTNQDEFSN
                     DFAAVPSGGYILGGYGYTYLKYIADDGIPKGMLIKTSESGCVEWSKKISSISSGSLYK
                     VIALQNGGYAISGLNDSISYVMRLD"
BASE COUNT          647 a          835 c          673 g          608 t
ORIGIN      
        1 gacaaacagg tcggggcgaa cacagaggct catcgccagg gcagaaaaag cccgcgcaag
       61 aaaagaccgc cgcgattata ccatatgaat tacaaagact tgtaacaggt agtgttctta
      121 actaaacgat atgggtgaaa atacggtacc gcgtttcggc ggaaattgtc agattcgccc
      181 cgggccgcgt ctcttcggca ggcaaatcta aagcgcaaca ccatgaacta cttcgacttt
      241 catgcccata tcctcctcaa acagctgttt gaagacaccc cggacgtaaa ccagaccgtc
      301 agcccgggcg atgtcaagct gatccccgag gattgctccg atcttccgtt catcatcgag
      361 acacagatac atcaaacaca gctgctctcg atgcacagcc ccgtactcat cggggcggca
      421 ctatatggca tggaaagctt tctcgcggcc gtcgtcgacc ccctgcgctc cttcctgaaa
      481 gacgcctcgc aggccaaact gtcccataat gtgctgacgc atatcgccgc ccccgactat
      541 aaaacttttt cgacatttac aaaagcgaat actcttgacc gttatctagg ggctccgaac
      601 ctcttcaaca ttctgggtaa ggccaacgca accgcaccgc tggaccccaa aaaggtgaat
      661 attttcttcg tcgtggaagg ctgtcattcg ctggtggact cgaagaacag ggtgaacctg
      721 gcgacaggcg acggatacga tccgaaggag atcctggcta atctcgatat ccttctgcgg
      781 caggtgacga tactggcggt gaacctcacc catctccagc agtccaatct atgcaaccac
      841 gccttcggca tgcagatagc cgaccccggc aacttctttc ccgccggcaa cggcttgacg
      901 gacgcaggac gaaccgtggt ccggggactc ttcgatcgcg gcatctgtgt cgacgtcaaa
      961 cacatgagct atctgtccag gctccagctg cgcaatgaga tcgatgccgg taaatatacg
     1021 aacccccaac ccctgctctg cacacatgcc ggttttacgg ggacatcgat agccgaatgg
     1081 cctaccttta tccgcaaaca gctaccggtc aacccgggta cgatgtatct cgagatcgcc
     1141 aagaacatcc aggcttacac gaaccgcacc cggcccgggg cgcccgcctt caatctttcc
     1201 accatcaatc tcttcgatga cgagatcgtc tggatcgtca aacatggtgg tatgatcgga
     1261 ctctccatgg accgccggat actcggtttc gtcagcacct ttgatgtgaa gcctaccggg
     1321 atcggcgccg gcagcgaatt tacggtggac aaggaatata tgtccatggc cgaatggaag
     1381 gtcctcggta taaacaagat cggggacgcc gtcgatgaag accattgcga gctgctcagc
     1441 gaggtcgggg aatttatgag cgccgccact cccatcccga tgcgcgatgc cttcttttat
     1501 gcgcatgtca tggtgcatat aaaacatttc ctgcaggtgt gtatcgacaa tggcatcccg
     1561 ctcgacaccg ccaggaaaca cctctgcatc ggctcggatt tcgacggggt gatcaacccg
     1621 ttcctcaatt cccgtacctg tctcgagatg cccgccttgc ggtccttcct tcagtcccgt
     1681 tttaagattt tcctcaaagg ccttaccgat tccgtccaat gggcggacca actggatatc
     1741 ggcggtttcc tcgaggacct gttcttcaat aacggcttta ccttcgttac cggccatctt
     1801 gcgggataac ttgcaagata tcagtgagtt tattattttg tcgcacaatt acccgatacc
     1861 gtgcgacctc cccatcgatt attttcaccg gccttactct gtggagctat cctgttttcc
     1921 atcgcttcat tcgcgcaaac cacccccgcg caaaccacct ccacgcaaac cacccccgac
     1981 acgatccatg ttgccggcaa ggtccttgtc cggaatcacg gtctttatat gtacgattcg
     2041 gtggagtggc aggccatgca atggcaaaag cagcaccgga aggagaccaa accttcgcgt
     2101 ccggcctttc tttcgaccga cagcgccgcc acgcccggcc aaacgctcct ggtgccggcg
     2161 cccctcatcc tgcaacagaa tacagcacat ccgctgaaca attgcggagt gagcgccagc
     2221 ttcacgccgg gtgacgattc ggtccttacg acgatgaccg ggcctgtcac gtttacgagc
     2281 accagcacca acgctacctc gcttacctgg ctggatgtcg ggatacccat ctccaacggc
     2341 aataccttca cgtgggggga gaaccagccc ggtctgtatg acatcatgct cgttgcgcag
     2401 aacggcgcct gtaccgatac ggcgatcgcc tattattata ttaaaggcgt tcagcccgcc
     2461 aacaggagaa acctatgggc ctattacggg accaatcagg acgaattttc caatgacttt
     2521 gccgcagtgc cgtcgggcgg ttatattctc ggcggctacg gctataccta tctcaagtat
     2581 attgccgatg acggtattcc caagggcatg ctgataaaga cctctgaatc cggttgtgtc
     2641 gaatggtcca aaaagatatc gtccatttcc tccggctcgc tatacaaagt aatcgcgctg
     2701 cagaacggcg gttatgccat cagcggactc aacgacagta tctcctatgt catgcggctg
     2761 gat
//
LOCUS       DASYSV010000054         5401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1166322, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000054 DASYSV010000000
VERSION     DASYSV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166322"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..667
                     /locus_tag="VGQ51_01625"
     CDS             200..667
                     /locus_tag="VGQ51_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV9035282.1"
                     /translation="MNSKQSAGILLYRRHTYLEVFLVHPGGPFWKNKDSGSWTIPKGE
                     FTDDEPALDAAVREFREETGVALKGPFQPLSPIRQKGGKRVYAWASPGDIDPAAIVSN
                     TFELEWPRRSGKQKTFPEVDKGGWFSLDAAKKAIIAAQIPLLDELEETQTPRH"
     gene            complement(781..1221)
                     /locus_tag="VGQ51_01630"
     CDS             complement(781..1221)
                     /locus_tag="VGQ51_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9035283.1"
                     /translation="MKKKFLVIDDDTDDRELFCEALASVDPGIHCIQAVDGKEALTRL
                     SSNEQPDIIFLDINMPIMDGWQFLSHLKKQDGHKNIPVIVYTTSSTQKDKRIANDLGA
                     LCFITKPHAYSRLQNILGVIVMHVNTQNYAKICEDVHAMGNGTS"
     gene            complement(1233..4334)
                     /locus_tag="VGQ51_01635"
     CDS             complement(1233..4334)
                     /locus_tag="VGQ51_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9035284.1"
                     /translation="MTPEFLSGGGEMGRRIREYDWSATPLGPVETWPQNLRTCIRIML
                     TSQQPIWIGWGKQLINLYNDPYRAILAGKHPGALGAPAEVVWKDIWRQIGPMLHHVTH
                     DNEGTYVESQLLLMERNGYPEETYYTFSYNPVLNDDGGTAGMICFNVDDTERIVGERQ
                     LRTLTGLGKRLTDCDSRQEALHRTLITLGENAHDFPFVLFYSGLTVGAVLTHSTHLGE
                     AAERVPSTIAPDEEGVLADALRRADRERRRQVISGIGEAMGPMPKGVWDVSPDKAVVL
                     PVFQAGESQAYGFLVIGVNPYRLPDEKFFGFCSMVVDQLMTSFADIHVLEMERKKAEA
                     LADIDRAKTVFFSNISHEFRTPLTLLISPIEELLSDPDTIGINRYRMGVAHRNALRMQ
                     RLVNTLLEYSRIEAGRMEGSYRRVNIVTLTKDLASTFRSAVEKAGMQLLMVTGAVDGD
                     VYVDTEMWERIMLNLISNAFKYSHRGSITVEVRQVDGRVQVSVTDTGVGIPGDQLEKI
                     FDRFHRVENSGGRSQEGTGIGLAMVRELVRLHHGTIHVRSVVEEGSVFTVSLPLGKDH
                     LPADRVFEPPEPAVKQYESEAFVAEAMKWIPATEEEWEEGALPERWGASILPGGDGKA
                     GEDGVEDEMGGFGEETEDDGRYTVVLADDNADMRGYVGRLLSSHYRVITARDGEDAFE
                     KMIRYRPDLLLSDVMMPRLDGFGLLKRVRQTPGLQHIPVILLSARAGEEAKVEGLETG
                     ADDYLVKPFGARELLARVDANIRITKERMNVMAAYAENLEIEVHKRTEELLSLNVSLE
                     RSNEDLQQFAHVASHDLKEPVRKVRTFTGRILEEYGSMLNQEARMFLIKIQQATARMG
                     SMIEGVLNYSMLNSHDQAMTLVDLNEVFINIESDLELVLQERNATIRRESLPMVEGAP
                     VLLYQLFYNIVGNSLKFSRRDSRPLITIASRVVEEVGKQIALFTITDNGIGFDQQQAG
                     RIFEAFTRLNSKDRYEGTGLGLALCKKIVERHHGSISATGVSGVGAVFVIKLPVRQME
                     RSI"
     gene            complement(4411..>5401)
                     /locus_tag="VGQ51_01640"
     CDS             complement(4411..>5401)
                     /locus_tag="VGQ51_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9035285.1"
                     /translation="TTSPTSPNYVTTATVTPRLEKKWVSIYSPVSYNVNGQLNWGAGV
                     RFGPVFIGSGTVLSSLLKQRLQTADAHIGLTIPIFQHGRTLEDKKHKADTVFNTKNLN
                     NDKDGDGVVDSKDNCPDSAGPVALIGCPDRDGDGVPDIKDKCPDVKGSANYQGCPAPD
                     SDGDSVNDDDDKCPLVKGDKSNFGCPAIRPELLARVKNAADRIYFVRAKANIEPVSFG
                     ELERVAEVLKSDSTLRLRIEGFTDSEGPDAREMRLSARRAQAVLKYLVVLGISPSRME
                     TIGYGKKKPLAPNDSPEGMARNRRVEMVLMNYPDKKKEDKPKPAGPGAAEATN"
BASE COUNT         1168 a         1736 c         1277 g         1220 t
ORIGIN      
        1 gacaaacagg tcggggcgaa cacagaggct catcgccagg gcagaaaaag cccgcgcaag
       61 aaaagaccgc cgcgattata ccatatgaat tacaaagact tgtaacaggt agtgttctta
      121 actaaacgat atgggtgaaa acacccattt ttcccccctc catccccatt attggcccct
      181 tatttgcgcc ccctcaccca tgaactcaaa acaaagcgcc ggtatattac tataccggcg
      241 ccatacatat ctcgaagtat tcctcgtcca ccccggaggc cccttctgga aaaacaaaga
      301 ctccggctcc tggaccatac caaaaggtga atttaccgat gatgaacccg cactcgatgc
      361 ggccgtccgc gaattcaggg aagaaacagg cgtcgccctc aaaggtccct tccaacccct
      421 cagccctatc agacagaaag ggggcaaacg cgtatatgcc tgggcgtccc ccggcgatat
      481 cgatccggca gccatcgtca gcaatacgtt cgagctggaa tggccgcgcc gctcggggaa
      541 acaaaagaca tttccggaag tggataaagg aggctggttc tcgctggacg ccgcaaaaaa
      601 agccatcatc gccgcccaaa ttcccctgct cgacgagctg gaagagaccc aaaccccgcg
      661 gcactaacgc atccggccat ccgcccaaac caagcgtccc ggcccaaacc ccacacccaa
      721 accgcaccgc aaaacccagc acccaaaccc caccgcacaa ccgcgccaaa ccccgccgca
      781 ctaactcgtc ccattcccca tcgcatgcac atcctcacaa atcttcgcat aattctgcgt
      841 attgacatgc atcacgatca cccccagaat attctgtagc ctcgaatacg catgcggctt
      901 cgtgataaag cacaacgcac ccagatcatt ggcaatacgc ttgtctttct gtgtcgacga
      961 ggtcgtatag acgatgacag gtatattctt atgcccgtcc tgtttcttca aatgcgaaag
     1021 gaactgccac ccatccatta tcggcatatt gatatccaaa aaaatgatat ccggctgttc
     1081 gtttgatgac agtctcgtca gggcttcctt accatcgacc gcttgtatgc agtgaatgcc
     1141 cgggtccacg gaagccaacg cctcgcaaaa caactcccgg tcgtccgtgt catcatcgat
     1201 gactaaaaat tttttcttca tgacttctgg ctttagatgc tcctttccat ttgtctcaca
     1261 ggcagcttta tcacaaaaac agctcccacg ccgctgacgc cagtcgcgga gatactgccg
     1321 tgatggcgct ccacaatttt cttgcataag gccaggccca atccggtgcc ttcgtatctg
     1381 tccttagagt tcagcctggt aaacgcttcg aagatgcgac cagcttgctg ctgatcgaac
     1441 ccgattccgt tgtccgttat tgtgaagaga gcaatttgct tgccaacctc ttcgaccact
     1501 cgtgatgcaa tggtaatcaa tggcctacta tctcttcttg aaaacttcaa tgaattcccc
     1561 actatgttgt agaacagctg ataaagcaac accggcgcgc cttccaccat gggcagcgac
     1621 tctcgccgga tcgtggcatt ccgttcctgc aacaccagtt cgagatccga ttcgatattt
     1681 ataaacactt cattcagatc gactaacgtc atcgcctgat cgtgactgtt cagcatggaa
     1741 taattcagca cgccttcgat catcgaaccc atacgcgcgg tggcctgctg tatcttgatc
     1801 aggaacatcc gcgcttcctg gttaagcatc gatccgtatt cttcgaggat cctgccggta
     1861 aaggtcctga cctttcgaac cggttccttg agatcgtgac tggcgacatg cgcgaattgc
     1921 tgcaggtcct cgttcgacct ttccagcgag acgttcagcg acaacaattc ttcggtgcgt
     1981 ttgtggactt ctatttccag gttttcggca taggcggcca taacgttcat cctctccttg
     2041 gtgatccgga tattggcatc aacgcgcgca agcaactccc ttgcgccaaa cggcttgacg
     2101 agataatcgt ccgcacccgt ctccaacccc tcgaccttcg cctcttctcc cgccctcgcc
     2161 gatagcagga tcaccggtat atgctgcaga cccggcgtct gccgcacacg cttcaacagc
     2221 ccgaatccat ccagcctggg catcatgacg tccgataata acagatcggg cctataacgt
     2281 atcatctttt caaaagcatc ctctccgtcc ctggccgtga tcactcgata atgggacgac
     2341 aacagccgcc cgacatatcc ccgcatatcg gcattgtcat cggccagcac gaccgtatat
     2401 ctgccgtcat cctctgtctc ctccccaaac ccgcccatct cgtcttcaac cccgtcttcc
     2461 cccgccttcc catccccacc cggtaaaata ctcgcccccc aacgctcggg caaagccccc
     2521 tcttcccatt cctcttccgt ggcgggtatc catttcatcg cttccgcaac aaaggcttca
     2581 gactcatatt gtttgaccgc cggctccggg ggctcgaaga cccgatccgc cggcaaatgg
     2641 tccttcccca gtggcaggct gaccgtaaat acggaacctt cctccacgac gctcctcaca
     2701 tggatggtcc cgtgatgcag acgaaccagc tcgcgtacca tggccagacc gataccggta
     2761 ccctcctggc tgcggccccc gctattctcg acacggtgaa atcggtcgaa gatcttttcc
     2821 agctgatccc ccgggatccc tacgccggta tcggtgacgc tcacctgcac cctgccgtcc
     2881 acctgccgga cctctaccgt tatgcttccc cgatggctgt atttaaaagc attcgatatg
     2941 agattgagca tgatccgctc ccacatttcc gtgtccacat acacgtcgcc atctaccgcc
     3001 cccgtcacca ttaacaactg catccccgcc ttctcgacgg ccgaccggaa ggtgctggcc
     3061 aggtccttcg tcagggtgac gatgttcacg cgacggtaag agccttccat ccgccccgcc
     3121 tctatccgcg agtactccag taaggtattc accagccgtt gcatccgcag cgcattccgg
     3181 tgcgctactc ccatccggta ccggttgata ccgatagtgt caggatcgct cagcagttct
     3241 tcgatcgggg aaatcagcag ggtcaacggc gtgcggaact catgactgat attggaaaaa
     3301 aagacggtct ttgccctatc gatgtccgcc agggcctccg ccttcttcct ttccatctcc
     3361 aggacatgga tatcggcaaa gctggtcatt agctgatcca cgaccatcga gcagaaaccg
     3421 aagaattttt catcgggcaa cctgtacgga ttcaccccga tgaccaggaa tccatacgcc
     3481 tggctctccc ccgcctggaa gacgggcaac acgacggcct tgtccggcga tacgtcccaa
     3541 acgcctttcg gcataggacc catggcttct ccgattccgg agatcacctg tcgccgccgc
     3601 tcccggtcgg cccggcgcaa cgcgtcggcc aggacacctt cttcgtcagg cgcgatagtc
     3661 gacggtaccc gttcggccgc ctctccgaga tgcgtggaat gcgtcagcac cgcgcccacc
     3721 gtcaacccgg aataaaaaag aacgaagggg aaatcatgcg cattctcgcc aagcgtgatc
     3781 agggtacggt gcagcgcctc ctgtcgggaa tcgcaatccg tcagccgctt gcccaacccc
     3841 gtaagtgtcc gcagctgccg ctcacccacg atccgttcgg tatcatccac attgaaacag
     3901 atcatccccg ccgttccgcc gtcatcattg agcaccgggt tatagctaaa ggtataatag
     3961 gtctcttccg gatacccgtt ccgttccatg agcaatagct gcgactcgac ataagtacct
     4021 tcgttatcat gcgttacgtg atgcagcatg gggcctatct gacgccagat gtccttccag
     4081 actacctccg ccggtgcgcc cagtgcgccc ggatgcttgc cggctaagat ggcccgatat
     4141 gggtcgttgt aaagattgat cagttgtttg ccccatccga tccagatggg ctgctgcgag
     4201 gtgagcataa tgcggataca ggtgcggaga ttctggggcc aggtctccac cggccctaag
     4261 ggtgtggccg accagtcgta ttcacggata cgcctgccca tttctcctcc tccagataga
     4321 aattcagggg tcatgttgcc taaaaataag caaaaaaaag gccatccttt ggacggcctg
     4381 gctcaggttt atctgatgta gcgtagtaga ctaattcgtg gcttccgcgg ctccgggtcc
     4441 tgccggcttt ggcttgtctt ccttcttttt gtccgggtag ttcattagca ccatctccac
     4501 cctccggttc cgcgccatcc cttcgggcga atcattcgga gcgagcggct tcttctttcc
     4561 gtatccgatg gtctccatcc gggaaggcga gatacccaat acgaccaggt atttgaggac
     4621 ggcctgcgcc cggcgggccg aaagacgcat ctcacgcgca tccggtccct cgctgtcggt
     4681 aaacccttcg atccgcaacc gcagggtgct gtccgatttg aggacctcgg ccacgcgttc
     4741 cagttcgccg aacgacaccg gttcgatatt ggccttggcc cggacaaagt agattcggtc
     4801 tgccgcattc tttacccggg ctagcagttc cggccggatt gcagggcagc cgaaattaga
     4861 cttatccccc ttgaccagcg gacacttgtc gtcatcgtcg ttcacgctgt cgccgtcact
     4921 gtcgggcgcc ggacaaccct ggtagttggc tgagcctttg acgtcgggac atttgtcttt
     4981 tatatcgggt acgccatcgc catccctgtc gggacatccg atcagcgcga cgggtcctgc
     5041 actatccggg caattgtctt tcgaatcgac gacaccgtcg ccatccttat cgttattgag
     5101 gttcttcgtg ttgaagacgg tatccgcctt gtgtttcttg tcttccaatg tccttccatg
     5161 ctggaaaatg ggtatcgtga ggccgatatg ggcatcggcc gtttgtaaac gttgtttgag
     5221 caggctcgac aggacggtgc cgctgccgat aaacaccggg ccaaaacgga ctcccgcccc
     5281 ccagttcagc tgtccattga cattatacga caccggggaa tagatgctga cccatttctt
     5341 ctccagccgc ggcgtcaccg tggccgtggt cacatagttg gggctggtcg ggctggtcgt
     5401 c
//
LOCUS       DASYSV010000055         4503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_526246, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000055 DASYSV010000000
VERSION     DASYSV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526246"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VGQ51_01645"
     CDS             <1..495
                     /locus_tag="VGQ51_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035286.1"
                     /translation="MLSRYALAGWIPAYVIYLILEKKWKHLSIITLTGILCFVLLFLL
                     PVGWDTFIRLASLPADYVSFAARVWKDSPDTFSTAPGFAWFFGPQKTWLLHRLLLCLS
                     FIVPLLFILYRRKRRATNLPLAALKLTLVIFYCFIDVPYLYLFYTSSFVSLIAVTLAV
                     PETA"
     gene            complement(452..1990)
                     /locus_tag="VGQ51_01650"
     CDS             complement(452..1990)
                     /locus_tag="VGQ51_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEV9035287.1"
                     /translation="MKLLSPAISRLARLRLGRIEHWMQNPVAVQREVLQHLVTSAQYT
                     EFGRKYNFSRIFSIREYKQAVPIHEYDDLKPYIQRIMNGEQNVLWNTPINWFAKSSGT
                     TSDKSKFIPVSEESLQDCHYKGAKDVLTMYYDFNPESDLLTGKGLVIGGSHTINQMNE
                     DVHYGDLSAVLLQNAPFWGNWIRTPELSIALLDEWEDKIERLAKNTIQENVTSISGVP
                     TWTMVLIRRILEITGKKTLAEVWPSLELYIHGGVAFTPYQQQFRNLIGKDINYLDMYN
                     ASEGFFAAQGNPNDEGMVLFLDHGIFMEFMPVEEYGKPHPETIGLDAVEIGHNYALVI
                     NTNGGLWRYLLGDTIQFTSRSPFRIKVSGRLKHFINAFGEEVIVDNTDKAVAVACEKT
                     GAVVNDYTAAPVYFSDGGNGAHEWLVEFEKKPADLTLFTTELDKALQNVNSDYEAKRH
                     KDIALRMPILHVLEKGVFTAWLKRKGKLGGQHKVPRLSNDRKYIDEIHAMLSQAPPAS
                     PLSD"
     gene            complement(2035..2817)
                     /gene="lptB"
                     /locus_tag="VGQ51_01655"
     CDS             complement(2035..2817)
                     /gene="lptB"
                     /locus_tag="VGQ51_01655"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEV9035288.1"
                     /translation="MSLTIHSQDLVKTYHNRTVVNHVSVNLKQGEIVGLLGPNGAGKT
                     TTFYMVVGLIKPDSGDVFLNDINITKLPMYKRAQLGLGYLPQEASVFRKLSVEDNIKA
                     VLEMTDLNRAQQREKLEALLDEFHLHHVRKNNGDSLSGGERRRTEIARALAVDPKFIL
                     LDEPFAGVDPIAVEDIQTVVARLKFRNIGILITDHNVNETLSICDRAYLLIDGKIFKH
                     GSAEELAEDEQVRRLYLGRNFELKRKDYLHEEARQAEEKNTH"
     gene            2934..3887
                     /locus_tag="VGQ51_01660"
     CDS             2934..3887
                     /locus_tag="VGQ51_01660"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEV9035289.1"
                     /translation="MKVIDHITNAKDTLVSFEILPPLKGKTISSIYEHLDPLMEFGPA
                     FINVTYHRSETMFKKKTDGTFEKVEVRKRPGTVGICAAIMNHYKVDAVPHLICGGFAK
                     RETEDALIDLHFLGIDNVLVLRGDAAKNESSFEPEPDGHRFAIDLLKQVACLNQGVYL
                     EEDIKNGGKTNFCIGVAGYPEKHFEAPNLETDLMYLKAKVAAGAEYVTTQMFFDNQKY
                     FDFVKVCREQGIHVPVIPGLKPITSKKQMSMLPKVFHVDLPTELSNEILQCKTDADVE
                     QVGTEWLIHQSRELKKAGVPVLHYYTLGKPKVIEKVVRATL"
     gene            complement(3918..>4503)
                     /locus_tag="VGQ51_01665"
     CDS             complement(3918..>4503)
                     /locus_tag="VGQ51_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035290.1"
                     /translation="RNHLEEYNADFAFQLSEFREGNLLFEIMQRKIWDKASTDSAGLM
                     KYYEGHKNNYWWNASADALLFTCRDAATAEGLKARLQTHPIVSWRQMVDSGATAIQAD
                     SGRYELAQIPNPGGTALTPQTYTAFSTNPADNSVSFAYILDIYRDRAPRNFRDARGFV
                     INNYQNWMEDQWIAELKKKYPVKIEEKVIAGLPR"
BASE COUNT          992 a         1205 c         1141 g         1165 t
ORIGIN      
        1 atgctgagcc gttatgccct ggcaggatgg atacccgcct acgttattta tcttatcctc
       61 gaaaaaaaat ggaaacacct gtcgatcatt acgctgacgg gcatcctgtg ttttgtattg
      121 ttgttccttc ttccggtggg atgggacacc ttcatacgac tcgccagtct tccggcggat
      181 tacgtttctt tcgctgcgcg ggtctggaag gatagtcccg acaccttttc aacggcgccg
      241 ggttttgcat ggttcttcgg gcctcagaag acctggctct tgcatagatt gctgctgtgt
      301 ctgagcttca tcgtgccgct gctgttcatc ctctatcggc gcaaacggcg cgccacgaat
      361 cttccgctgg cggcgctaaa acttacgttg gtgatctttt actgttttat cgacgtcccc
      421 tatctctatc ttttctatac ctcgtctttt gtcagtctga tagcggtgac gctggcggtg
      481 cctgagacag catagcatgt atctcatcta tgtatttacg gtcattggac aatcgaggta
      541 ctttatgctg tccccccagc ttgcccttgc gtttcaacca ggctgtgaaa acgcccttct
      601 ccagcacatg caggataggc atccgcagcg caatatcctt atgtcgtttg gcctcgtaat
      661 cgctattgac gttctgaagg gctttatcca gctcggttgt gaacagggta agatcggcgg
      721 gttttttttc aaactccacc aaccactcat gtgccccatt acccccatcg ctgaaataga
      781 ccggcgcggc ggtataatcg ttcacgacgg cgcccgtctt ttcgcaggcg acagcaaccg
      841 ccttatcggt attgtcgacg atcacctcct cgccgaaggc gttgatgaaa tgtttgaggc
      901 gtccgctgac tttgatccgg aacggacttc gtgacgtgaa ttggatggta tcgcccagca
      961 aatagcgcca gagaccaccg ttagtattga tcaccagcgc gtagttatgg ccgatctcca
     1021 ccgcgtcgag cccgatcgtc tcgggatgcg gcttgccgta ttcctccacc ggcataaact
     1081 ccatgaagat gccatgatcc aggaaaagga ccatgccctc gtcattggga ttgccctgcg
     1141 cagcaaaaaa tccttcgctg gcattgtaca tgtcgagata gttgatgtcc ttcccgatga
     1201 ggttccggaa ttgttgttga taaggcgtga aggctacccc accatggata taaagctcca
     1261 gcgaaggcca tacctccgcc agcgtttttt ttcccgtgat ctcgaggata cgcctgatca
     1321 acaccatcgt ccaggtcggc acgcccgaga tcgaagtcac attctcctgt atcgtattct
     1381 ttgccagccg ttcgatctta tcctcccatt cgtcgagcaa tgcgatgctc agctcgggcg
     1441 tccggatcca gttgccccag aacggcgcgt tttgaagcag gacggcgctg aggtctccat
     1501 aatgaacatc ttcattcatt tgattgatag tatgactgcc cccgatcacc agccccttac
     1561 ccgtcaacag atcagactcg ggattgaaat cataatacat ggtcagtacg tcctttgcgc
     1621 ctttatagtg gcaatcctgc aggctttctt cgctgacggg gatgaacttg cttttgtcgc
     1681 tggtggttcc gctgcttttg gcaaaccagt tgatgggggt attccagagg acgttctgtt
     1741 ccccgttcat gatacgctgg atgtagggct tcagatcgtc gtattcatgg atgggaacgg
     1801 cctgtttgta ttcgcgaatg gagaagatcc gggagaaatt gtatttgcgg ccgaattcag
     1861 tgtattgagc cgaggtgacg agatgctgca gcacctcccg ttgtacggct acggggttct
     1921 gcatccagtg ttcgatgcgg ccaagacgca atctggcgag tctcgatatg gccgggctca
     1981 aaagtttcat ggtataccag caaaataact aatcggccgg acattcagga cattttagtg
     2041 ggtattcttt tcctccgcct gtcgtgcttc ttcgtggaga tagtcctttc ttttcaattc
     2101 gaaattgcgt ccgagataaa gacgtctcac ctgctcgtcc tccgcgagtt cctccgccga
     2161 gccatgtttg aagatcttgc catcgatgag aagataggcc ctgtcgcata tggaaagcgt
     2221 ctcgttcacg ttgtggtccg tgatgaggat gccgatattc ctgaatttaa gccgcgccac
     2281 caccgtttgg atatcttcca cggcgatggg atcgacgccg gcaaatggtt cgtcgagcag
     2341 gatgaatttc gggtcaacgg ccagcgcccg ggcgatctcg gtccgccggc gttctccgcc
     2401 tgacagcgag tcaccgttgt tcttgcgtac atgatgcagg tggaattcat ccaacagggc
     2461 ttccagcttt tcgcgttgct gtgcgcggtt aagatcagtc atctccaata cggccttgat
     2521 attgtcttct acgctcagct tgcgaaagac cgaggcctcc tgcggcagat agccgagtcc
     2581 aagctgggcc cgtttgtaca tgggtaattt cgtgatattg atatcattga gaaagacatc
     2641 acccgaatcg ggcttgatca ggccgaccac catatagaag gtcgtagtct taccggcgcc
     2701 attgggtccg agcaaaccta cgatctcgcc ttgcttaagg ttcaccgaga cgtgattgac
     2761 caccgtcctg ttgtgatacg tttttacaag gtcctgggaa tgaatggtca gactcacgta
     2821 tttctaaatt tgcccgcaag atacgccccg ccggtcatgt ggcgaagccc ggggtttaaa
     2881 tttataggtg ttttaagagt tatcaactaa gtttgcggga ttattcgcgc aatatgaagg
     2941 ttatagacca cattaccaac gcaaaagata ccctggtttc gttcgagatc cttcctcccc
     3001 tgaagggaaa aaccataagt tccatctacg aacatctcga tcctttgatg gaattcggcc
     3061 ctgcgttcat caacgtcacc tatcaccgca gtgaaacgat gttcaagaag aagacggacg
     3121 gcacttttga aaaggtcgag gtccgtaaac gtcccggtac cgtcggcatc tgcgccgcga
     3181 tcatgaacca ttataaggtc gatgccgttc cgcatctcat ctgcggcgga tttgccaaac
     3241 gcgagacaga agacgctctg atcgatctgc atttcctggg tatcgacaac gtactggtac
     3301 tgcgcggaga cgccgcaaag aatgaaagct ctttcgaacc cgaaccggac gggcatcgct
     3361 tcgcgatcga tcttcttaaa caggtggcat gtcttaacca gggcgtctat ctcgaagaag
     3421 atatcaagaa tggaggtaag accaatttct gcatcggcgt cgcaggctat ccggaaaaac
     3481 acttcgaagc acccaacctc gaaacggacc tgatgtacct gaaggcgaaa gttgccgcgg
     3541 gcgcggaata tgttactact cagatgttct tcgacaacca gaaatacttc gattttgtaa
     3601 aggtctgtcg cgagcagggt atccatgttc ctgtgatacc gggtctgaag cctattacga
     3661 gtaaaaaaca gatgagtatg ctgccgaagg tgtttcatgt agatctgccc accgaactat
     3721 ccaacgagat cctgcaatgc aagaccgatg cggatgtcga gcaggtgggt accgaatggc
     3781 tgatccatca gagcagggag ctcaaaaaag caggcgttcc cgttctccat tattatacgc
     3841 ttggaaaacc caaggtgatc gagaaggtag ttagagcgac cttgtaggcc ggaagggcat
     3901 acccgcgaag catatggtca acgcggcagt ccggcaatca ccttctcctc gatcttcacc
     3961 ggatacttct ttttcagctc ggcgatccac tgatcctcca tccagttttg gtaattattg
     4021 atcacgaaac cccgcgcgtc ccggaaattt cgcggagccc gatccctata aatatccagg
     4081 atataagcga agctcaccga attgtccgcc ggattggtcg agaatgccgt atacgtttgc
     4141 ggcgttagcg cggttccccc cggattgggt atctgtgcca gctcatacct ccctgaatcg
     4201 gcctgtatgg ccgttgcgcc gctatccacc atctgccgcc agctgacgat gggatgggtc
     4261 tgaagccggg cctttaggcc ttccgcggta gcggcatccc ggcaggtgaa cagcagggcg
     4321 tcggcgctgg cattccacca atagttgttt ttatgaccct cataatattt cattaacccg
     4381 gcgctgtccg tcgacgcctt atcccatatc tttcgctgca tgatctcgaa gagtaggttg
     4441 ccttccctga attcgctcag ctgaaacgcg aaatccgcgt tatattcttc cagatgattc
     4501 cga
//
LOCUS       DASYSV010000056         7845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1934570, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000056 DASYSV010000000
VERSION     DASYSV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934570"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGQ51_01670"
     CDS             <1..182
                     /locus_tag="VGQ51_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035291.1"
                     /translation="PVDLEKIVKKDKPIPSPIERLMKARWVDDPRHYIYCSAFPGQRV
                     KLSTRSGLISHLPAN"
     gene            319..915
                     /locus_tag="VGQ51_01675"
     CDS             319..915
                     /locus_tag="VGQ51_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035292.1"
                     /translation="MLSHDKPIIPIDSSYFLTLNTVDKIDVFTRPAYKQVIADSLNYF
                     IGTQGLVVYSWCLMTSHLHLIVRTREASALAYFERDFKKHTTPIILKTIEMEMDFRRD
                     WMMQRFEDYGKPLRRIEKFLLWQNCSSPLRIDCQQPRYLLDRIAHIHENPVREKIVEQ
                     PEAYIFSSARDYAGIRGLVNVRVVQQQGIFGMKALPVN"
     gene            complement(901..1257)
                     /locus_tag="VGQ51_01680"
     CDS             complement(901..1257)
                     /locus_tag="VGQ51_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEV9035293.1"
                     /translation="MKKLYHLSSCTTCQRILKETGAAQKGFVLQDIKTQPITPAQLDE
                     MKEKAGSYEALFSRRALKYKELGLKDQKLTEADYRRLILEEYTFLKRPVVVAGKRLFA
                     GSEKATVAALKVLIDR"
     gene            1321..1836
                     /locus_tag="VGQ51_01685"
     CDS             1321..1836
                     /locus_tag="VGQ51_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9035294.1"
                     /translation="MSNQDRQEVFISLVNANRSILYKICRMYGATEPDRQDLFQEIVI
                     QLWRSYPGFRGDARFSTWLYRIALNTAISDLRKQRRVGPSFDPDQLSIPMQDMQYVND
                     KEEQLQQLYAAIDRLTEVEKALTMLYLEDKSYQEMEEILGISQNNLRVKMNRIKEKLR
                     KMTKEAAYGIR"
     gene            1823..2431
                     /locus_tag="VGQ51_01690"
     CDS             1823..2431
                     /locus_tag="VGQ51_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035295.1"
                     /translation="MELDNLKTIWKEQETLAPVTDPEHLAWLLQMKSRGPIARMRRNL
                     RVEALLMVITYIPTIIAYLVLFAGRLSLISLFLFVVLCFYAVYFYRKDRLLIKMQCVT
                     CEVRSNLAGQVKALRKYIRFYIWSGTLVILIAMVVACCTIRYAMQLKSMPIHWWFEPP
                     FLLAILTPFAFGLLHLNKWYASKLYGRHIEKLDAMLREMDED"
     gene            complement(2454..3227)
                     /locus_tag="VGQ51_01695"
     CDS             complement(2454..3227)
                     /locus_tag="VGQ51_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV9035296.1"
                     /translation="MPTRLKKTSLWKLVLVTMAAAVLVMLGFTAYDWWLERRAHFVRY
                     AAFGIDIPVNYTIHGIDVSKYQNIIDWGSVKGMKVGDVQMSFAFIKATEGLDNEDAYF
                     KRNWKKVKDAGLARGAYHFFLATKSGKAQAENFINSVELAPGDLPPVLDIEQTYGIPS
                     EKMRERAKEWLTTVQDYYHVQPIIYTNVDFYKQYLKDDFDGYPLWVAHYLRQERPNIY
                     RTWAFWQHSESGRVNGILTRVDFDVFNGDSSEFRRLLIN"
     gene            3265..4296
                     /locus_tag="VGQ51_01700"
     CDS             3265..4296
                     /locus_tag="VGQ51_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HEV9035297.1"
                     /translation="MPDLNRYDTINFLRKLTPRRMINGIKVLSSYYVSKWTKKAVQWG
                     YPISLSFEPTTSCNLRCPECPSGLRAFTRPTGMLGDDFFRQTIDQLSDHLLYLVFYFQ
                     GEPYLNPSFLDMVTYASSRGIYTATSTNAHYLNDRNARKTVESGLDRLIISIDGTTQD
                     VYEQYRVGGRLDKVLEGARNIVKWKKELKSRKPFVFFQFLVVRPNEHQLDDIRRLAKE
                     IGVDQVRFKTAQVYDYQKDPNGLIPRQEKYSRYRKDANGEYVFKNGLENHCWRLWHTT
                     VITWDGLVVPCCFDKDAQHTLGDLKGKPFKEIWHDDRYVEFRRRVLQSRKNIDICANC
                     SEGTKVWED"
     gene            4352..4831
                     /locus_tag="VGQ51_01705"
     CDS             4352..4831
                     /locus_tag="VGQ51_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV9035298.1"
                     /translation="MPLSPSAWTRLEYQHRTIRELIEDFSEQQLKQRVDPSKWSVFEN
                     IVHLASYQPIFLLRLDRILNGDLPAFTRYTADDDPQFSIDLELPLHSLLDYIDTRRAD
                     LVLKLQGMSETELSRTGVHPAFGKMNVVRWTEFFLLHEAHHIYTIFRLVQQMRKELR"
     gene            4857..6461
                     /locus_tag="VGQ51_01710"
     CDS             4857..6461
                     /locus_tag="VGQ51_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEV9035299.1"
                     /translation="MAFKIAPALLIAVLIVLLPGRTFSQTDSATSPVTAAMAPKNETV
                     PTRPKYEFRAVWVASVENIDWPSKKGLPVDSQKVEFTRLLDLHKRNGFNAVVVQIRPA
                     ADAFYPSPYEPWSEWLTGVQGQAPSPYYDPLQFMIEETHRRGMEFHAWINPYRAVKTI
                     GKSSVAADHVTRQHPDWFVRFENTLYFDPGNKAVQAYVTNVVRDIVHRYDIDAIHMDD
                     YFYPYDIVEGGPRGKDFPDQPSYAEYGNGMSKGDWRRSNVDSVILRVGKAIKEEKSWV
                     KFGISPFAIWRNSNKDPEGSDTHGGVTDYDNLYANILLWLKQGWIDYVVPQIYFEFTQ
                     PHAPYGPLLEWWAHHSYGRQCYIGLGVYRAGSNAAWRDPTLLPRQVQALRSYPTVQGA
                     VYFSSRSLEDDLLGWSDSLRNNYYDYPALIPPMSWIDSIKPQAPTAHIEYDPKGLSAT
                     AWLSKGEPGDSLRGYAIYQSDSANIDIDSSRIFDFIPYDPVAGFTIHPGPAEQKGAVH
                     YYFATAISRTNVESLPVPLVFSNFGH"
     gene            6473..7693
                     /locus_tag="VGQ51_01715"
     CDS             6473..7693
                     /locus_tag="VGQ51_01715"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV9035300.1"
                     /translation="MPGFELFGPEERKEVNDVLETGILMRYGFDGPRKGIWKAKELET
                     AIGEKLGSRYTQLVSSGTAALTTAFAALGIGAGDEVIMPTFTFVASFECVLSVGAIPV
                     LVDVDETLTLDPAAVRRAITPRTKCIMPVHMCGAMADLDALRNVCKEHKLILLEDACQ
                     SFGGRYKGRALGTIGDAGTFSFDFVKIVTCAEGGAVVTNDESLYTKLDGYSDHGHDHK
                     GVDRGADLHPFIGYNFRISELHAAVGLAQVRKLDKFIAMQRKTHSRLQAILSEVPEIG
                     FRSIPDPDGDICSFLSWFLPTEEITREVVAELRQQGILAGNFYWYDNSWHYIRKWDHL
                     RNATTLNPLSESQKQGLLALNQTSFAASDRVMSRCISTAIGLLWTEQQTEEKGTKIVA
                     AVRKVLSRVKTPVV"
BASE COUNT         1852 a         2159 c         1988 g         1846 t
ORIGIN      
        1 agcctgtcga tctggaaaag atcgtcaaga aggacaagcc cattccatca cccatcgagc
       61 ggctgatgaa ggccagatgg gtcgatgatc cgcgccatta tatttactgc agcgccttcc
      121 cggggcagcg ggtaaaatta tctacaaggt ccggtctgat cagccatctc cccgccaatt
      181 gagaaattgg cgatatgggt aaaaaataag ggaaagttgg caaaccaatc tcccggattg
      241 ttgtaacttt aaaagcattc ccaccccgaa taaccgtgct cattcgtctg tattattttg
      301 ccaccaaaat aagaggttat gctttcacac gacaagccca tcattcccat tgactcttcc
      361 tactttctga cgcttaatac agtagacaaa atagatgttt tcacccgccc ggcttataaa
      421 caggtgatcg cagactccct gaactatttc atcgggacgc agggtctcgt cgtttattcc
      481 tggtgcctga tgaccagcca tctccacctc atcgtacgga cgcgggaagc ctccgcactg
      541 gcttatttcg agcgggattt caaaaaacat acgactccga tcattctgaa aacgatagaa
      601 atggagatgg atttccgccg cgattggatg atgcagcgtt tcgaggatta tggcaagccg
      661 ctcagacgga tcgagaaatt ccttttatgg cagaactgca gcagcccgct gcgcatcgac
      721 tgtcagcagc cgcgttatct gctcgaccgc attgcgcata tccatgagaa tccagtacgg
      781 gaaaagatcg tcgagcagcc ggaagcctat attttcagca gtgcacgcga ttatgccggg
      841 atccgcgggc tggtgaacgt acgcgtcgtc cagcagcagg gcatctttgg aatgaaagcc
      901 ttaccggtca attagtactt tcagagccgc caccgtcgcc ttctcactcc ctgcgaaaag
      961 ccgttttccg gccacgacaa cagggcgttt caggaaagta tattcttcca ggatcagccg
     1021 gcggtaatcg gcctcggtca gcttttggtc ttttaacccc agctccttgt atttcagcgc
     1081 acgacggctg aacagcgcct cgtaggaacc cgccttttcc ttcatttcgt ccagttgcgc
     1141 cggcgtgatc ggctgggtct tgatatcctg gaggacaaag cccttttgcg cggccccggt
     1201 ctctttcaat atccgctggc aggtggtgca ggaggaaaga tgatacagct tcttcatgag
     1261 ggcgaatgta atatttttcg cgggtcaccg acaaatagta ttattgttta aaccctcgtt
     1321 ttgtccaatc aagaccgaca ggaagttttt atcagcctgg tgaacgccaa ccgaagcata
     1381 ctctataaga tatgcaggat gtatggcgct acggaacccg accgccagga tctgttccag
     1441 gagatcgtca ttcagctttg gcgatcgtac cccggattcc ggggagacgc ccgtttcagc
     1501 acctggctat accgcatcgc gctgaatacc gccatatccg atctgcgcaa acagcggcgg
     1561 gtcgggccgt ccttcgatcc cgaccagtta tccattccta tgcaggatat gcaatatgtc
     1621 aacgacaagg aagaacagct acagcaatta tatgccgcca tcgatcggct caccgaagtg
     1681 gaaaaagcgc ttaccatgct ctacctggaa gacaaaagct accaggaaat ggaagagatc
     1741 ctgggtattt cacaaaacaa tctgagggtt aaaatgaatc ggatcaagga aaaattacgt
     1801 aaaatgacga aggaggccgc ttatggaatt agataacctg aaaacgatct ggaaggaaca
     1861 ggagaccctg gccccggtga ccgatcccga acatctggcc tggctgctcc aaatgaaatc
     1921 gcgcggcccc attgcccgta tgcgtcgtaa tcttcgcgtc gaagccctcc tcatggtcat
     1981 tacctatatt cctaccatta tcgcctacct ggtcctgttc gccggccggt tgtcgctcat
     2041 ctcgctattc ctctttgtcg tcctgtgttt ttatgccgta tatttttatc ggaaagaccg
     2101 gctgctgata aagatgcaat gcgtcacctg tgaggtgcgg tccaaccttg caggacaggt
     2161 aaaggcactg cggaagtata tccggttcta tatctggtcg ggtacactgg tcatcctgat
     2221 cgctatggtg gtggcctgtt gcacgatccg gtatgcgatg cagctgaagt cgatgcctat
     2281 ccactggtgg ttcgagccgc ccttcctgct ggcgatattg acacccttcg ccttcgggct
     2341 gttacacctc aataagtggt atgcgagcaa actgtatgga cgccatattg aaaaactcga
     2401 cgcgatgctt cgcgaaatgg atgaggattg atgcccccca gccctggccg gccctaattg
     2461 atcagcaatc ttctgaattc cgaactgtcc ccattgaaca cgtcgaaatc cactcttgta
     2521 aggatgccgt tgacacgccc cgattcgctg tgttgccaga aggcccaggt ccggtagata
     2581 ttgggtcttt cctgcctgag atagtgagct acccacaacg ggtacccgtc aaaatcatcc
     2641 ttgaggtatt gtttgtagaa atccacattg gtatagatga tcggctgaac atgataatag
     2701 tcctggacgg tcgtaagcca ttcctttgcc cgttcgcgca tcttttcgga cggtattcca
     2761 taggtttgtt cgatatccag cacgggaggc agatcgcccg gcgccagctc aacggaattg
     2821 atgaaattct cggcctgcgc cttcccgctc ttggtagcca ggaaaaaatg ataggctccc
     2881 ctggccagac cggcatcctt cacctttttc cagttgcgct tgaaataggc gtcttcatta
     2941 tccaggccct ccgtcgcctt gatgaaagca aagctcatct gtacgtcgcc gaccttcatg
     3001 ccctttacgg aaccccagtc gataatgttc tggtatttcg agacgtcgat gccatggatg
     3061 gtataattga cgggaatatc gataccgaat gcggcatacc ggacaaaatg ggctcttctc
     3121 tccagccacc agtcatatgc ggtgaatcca agcataacca gcacggcggc ggccatagtg
     3181 accaatacga gcttccataa agaggttttc ttcaatcttg ttggcatagg ttcctacaaa
     3241 cataattagt atttttatac ttccatgccc gatcttaaca ggtacgatac cattaatttt
     3301 ctcaggaagc tcacgccgcg gcggatgatc aacgggataa aggtcctaag cagctactat
     3361 gtcagcaagt ggaccaaaaa agccgttcaa tggggctatc ccatttcatt atccttcgaa
     3421 cctacaacgt cctgtaatct ccgctgtccc gagtgtccca gcggattgcg cgctttcacc
     3481 cgccccaccg gcatgctcgg ggatgatttt ttccggcaga cgatcgatca gctctccgac
     3541 cacctgctct acctcgtctt ttattttcag ggtgagccct atcttaaccc atctttcctg
     3601 gatatggtca catatgcgtc ctccaggggc atctataccg ccacttctac gaacgcacat
     3661 tatctcaacg accgcaacgc cagaaagacc gtagagagcg gactcgaccg cctcatcatc
     3721 tccatagacg gtacgacaca ggatgtctac gaacaatacc gggtcggagg cagattggat
     3781 aaggtcctgg aaggtgccag gaacattgtc aaatggaaaa aagaattgaa gagccggaag
     3841 ccctttgtct ttttccagtt cctcgtagtg cggcccaacg aacatcagct cgacgatatc
     3901 cggcgtcttg ccaaggagat cggcgtggat caggtgcgtt tcaagacggc gcaggtatat
     3961 gactaccaaa aggaccccaa cggcctgatc ccacggcagg aaaaatacag ccggtatcgg
     4021 aaggatgcca atggagaata tgtctttaaa aatgggctgg agaaccattg ctggcgtctc
     4081 tggcatacga ccgtgatcac ctgggacggc ctcgtggtcc cgtgctgttt cgataaagac
     4141 gcgcaacaca cgctgggcga tctgaagggt aagccgttca aagagatctg gcatgacgac
     4201 cgttatgtgg agtttcgtcg ccgggtactg caaagcagga agaatattga catctgcgcc
     4261 aactgcagcg aggggacgaa ggtctgggaa gattaggagt gcgaagatgc cggcgggcat
     4321 gcgccacagg gagattacct aaaccgatca tatgcctcta tcaccatccg cctggacccg
     4381 gctggaatac cagcatcgga ccatccgcga gctcatcgaa gatttctccg aacagcagct
     4441 gaaacaacgc gtagacccct ctaaatggag cgtcttcgag aatatcgttc atctcgccag
     4501 ctatcaacca atttttctgc ttcgtctcga caggatactg aatggggacc tgcctgcatt
     4561 tacccgctat acggcggacg acgatcctca attctccatc gatctggaat taccgctgca
     4621 ttctttgctc gactatatcg acacccgtcg cgcagatctg gtcctcaaac tgcaaggcat
     4681 gagcgaaacc gagctttccc ggacgggcgt gcatcccgca ttcggaaaaa tgaacgtcgt
     4741 ccggtggacg gagttctttc tcctgcacga agcgcaccat atttatacaa tttttaggct
     4801 ggtacagcag atgcgcaagg agttgcgatg aggtttttcg ttaattttat cggatcatgg
     4861 cattcaagat cgctccggca ttgctcatcg ctgttttaat cgtgctcctt cccggcagaa
     4921 cgttcagtca gaccgacagc gccacttcac ccgtcacagc ggcgatggcg ccgaaaaatg
     4981 agaccgttcc gacgcgaccc aaatatgaat tccgagccgt ctgggtagcc tctgtggaga
     5041 acatcgactg gccttccaaa aagggccttc cggtcgacag ccagaaagta gaattcacgc
     5101 gtctgctcga tctgcacaaa cgcaatggct tcaatgcggt ggtcgtccag atacggccgg
     5161 cagccgacgc cttctatcct tctccctatg agccctggag cgaatggctc accggcgtcc
     5221 agggtcaggc cccttctcct tattacgatc ccttgcaatt catgatcgag gagactcacc
     5281 ggcgggggat ggagttccat gcctggatca atccttacag ggccgttaag accatcggga
     5341 aatcgtccgt cgccgccgac catgtcaccc ggcagcatcc ggattggttc gtgcggttcg
     5401 agaatacgct ttatttcgac cccggcaaca aggcggtgca ggcctatgtc acgaatgtcg
     5461 tccgtgatat cgtgcatcgc tatgacatcg acgcgatcca tatggacgac tatttctatc
     5521 cgtacgatat cgtcgaggga ggcccgcggg gaaaagattt tcccgaccag cccagctatg
     5581 ccgaatacgg caacggcatg tcgaagggtg actggcgacg cagcaatgtg gattccgtca
     5641 tcctgcgtgt cggcaaggcc attaaggaag aaaagtcgtg ggtcaaattc ggcatcagcc
     5701 cttttgccat ctggcgcaac agcaataaag acccggaagg cagcgacacg catggcggcg
     5761 ttaccgatta cgacaactta tatgcgaata tcctgctttg gctgaaacag ggctggatcg
     5821 attatgtcgt acctcagatc tatttcgagt tcacgcaacc ccatgcgccc tatggtccgc
     5881 tgctcgaatg gtgggcacat cacagctatg gcagacaatg ttacatcggt ctgggtgttt
     5941 accgcgcagg ctccaatgcg gcctggcgcg atccgaccct gcttccgcgg caggttcagg
     6001 cgctccggag ctatccgacg gtccaggggg cagtctattt cagcagcaga tctttggaag
     6061 acgatcttct cggatggagc gacagcctgc ggaacaacta ttacgactat cctgccctga
     6121 taccgcccat gtcctggatc gactcgatca agccgcaggc gccgaccgcc cacatagaat
     6181 acgatccgaa aggactctcc gctaccgcgt ggctttcgaa aggcgagccc ggcgattcgt
     6241 tgcggggata cgccatctat caatccgatt cggcaaacat tgatatcgac agttcccgca
     6301 ttttcgattt catcccctat gatcccgtcg cgggattcac catccatccc ggaccggccg
     6361 aacaaaaggg tgcggtacat tattatttcg cgacagccat cagccggacc aatgtggaaa
     6421 gtctgcctgt tccgctggtt ttctctaact ttggacacta aatcattata ctatgcccgg
     6481 ctttgaacta ttcggccctg aagaaagaaa ggaagtcaat gatgtattgg aaaccgggat
     6541 cctcatgaga tatggattcg acggcccacg taaaggcatc tggaaggcga aggagctgga
     6601 aaccgccatc ggtgaaaaat tgggtagccg gtatacacaa ttggtcagca gcggtacggc
     6661 ggctttgacg acggcctttg ccgcgctcgg tatcggtgcc ggcgacgagg tgatcatgcc
     6721 cacttttact ttcgtggcca gctttgaatg cgttctaagt gtgggcgcaa tacccgtgct
     6781 ggtagacgta gacgaaaccc tcactctcga tcctgctgcc gttcgccgtg ccattacgcc
     6841 tcgtaccaaa tgtatcatgc ctgtacatat gtgtggcgcc atggccgatc tcgatgccct
     6901 gcgcaatgtc tgcaaggaac ataagctgat cctcctggaa gacgcttgtc agagtttcgg
     6961 aggccgttat aaaggtcgcg cgctgggcac gatcggcgac gccggaacat tctctttcga
     7021 ttttgtcaag atcgtcacct gtgccgaagg gggcgccgtc gttaccaatg acgaatcgct
     7081 atataccaaa ctggacggct actcggatca tggacatgat cataaaggcg tcgaccgcgg
     7141 tgccgatctt catcctttca tcggctataa cttccgtatc tccgaactgc acgccgccgt
     7201 gggtctggcg caggtgcgca agctggacaa attcattgcg atgcaacgga agacgcatag
     7261 ccggttgcaa gccatcctct ccgaagtgcc ggagatcggt ttccgcagca tacccgatcc
     7321 cgatggcgat atctgcagtt tcctgagctg gttcctgccg actgaagaga tcacccgcga
     7381 agtcgtcgcc gagctgcgtc agcagggcat actggcaggc aacttttact ggtatgacaa
     7441 cagctggcac tatatccgta aatgggacca tcttcgaaac gcaactaccc tgaacccgct
     7501 gtccgaatcg cagaaacagg gtctgctggc attgaaccag acgtcctttg ccgccagcga
     7561 ccgggtcatg agccgttgca tttctactgc catcgggctc ttgtggaccg agcagcagac
     7621 agaagaaaaa ggaacgaaga tcgttgccgc cgtccggaag gtattgtcca gagtgaaaac
     7681 gccggttgtc tgatccctcc gaagaaacga ttccctatat gttggaaaca ctgaaacggg
     7741 tccctctttt cgttcccttg ctttccatct tttttgttct gcacggggtc ctcgagaatt
     7801 tcgggttcat cccggtcatg gatgctttta tcctgatcat cgtct
//
LOCUS       DASYSV010000057         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_653782, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000057 DASYSV010000000
VERSION     DASYSV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653782"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1586
                     /locus_tag="VGQ51_01720"
     CDS             <1..>1586
                     /locus_tag="VGQ51_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEV9035301.1"
                     /translation="DRKALPAPEAASDANYIGPSGEIEERLTDIWADVLKTDRSLIGA
                     NSDFFKLGGHSLNAIMLVRRVEKFFGRSIPLRVFYHLSTVREMARFLHTFSGDEDRTI
                     PAAGEKEYYTLSSQQKRLFFLHELDRYSLAYNESAEYGLEGPLDTERLSLVFQRLVDR
                     HEILRTSFGLVDGTPVQKVAAAAKLPIEWLPEGRPSEETLRKFIRPFDLSEPVQLRVG
                     LMRLAPERHLLIVDLHHIITDGISKGVLINDFIAIYQDEPLPPPALHYKDFAEWQQGP
                     GQRRLSAVQREFWLEQFSGEIPVLELPADFPRPPVKDYAGGRFMFALGREDTAALKSL
                     ALGERVTLYSVILALYNVLLARLSNQDDIVVGTPVAGRPHADLERVMGMFIDTIPVRN
                     HPAENTGFRQFLQDLHSRTTACLDNQAYPYEQLVEELKVPRNTGRNPLFDALFIFHNF
                     EMFLLEMRGLVIRRMERSHPISKFDLTLEAVESPEGLSCTFEYSTNLFRRDTVERFAG
                     YFQRIVAAVTEDPEVKLGAI"
BASE COUNT          334 a          426 c          474 g          352 t
ORIGIN      
        1 gaccggaagg cgttgccggc gccggaagcg gcgtccgatg cgaactatat cgggccctcg
       61 ggagagatcg aggagaggct gacggatata tgggccgatg tcctgaagac cgacaggtcg
      121 ctgatcggcg ccaacagcga ttttttcaaa ctgggcggac attcgctgaa tgccatcatg
      181 ctggtaagac gcgtagaaaa attctttgga aggagcattc cgctcagggt attttaccat
      241 ctttccacgg tccgggagat ggcccgtttc ctgcatactt tttccggcga tgaggacagg
      301 accatccctg ccgcaggaga aaaggaatat tatacgctat cctcgcagca aaagcgcctg
      361 ttctttctcc atgaactcga caggtactcg ctggcctata acgaatccgc ggaatacggc
      421 cttgaaggac cattggatac ggaaagactg tcgctggtct ttcaacgact ggtcgaccgg
      481 catgagatcc tccggacctc tttcggcctg gtcgatggaa cgccagtgca gaaagtcgca
      541 gcagcggcga agctgccgat cgaatggctg cccgaaggaa ggccttctga ggagaccctg
      601 cggaaattca ttcgcccatt cgatctgagt gagccggtgc agctccgggt tggattgatg
      661 cgtctggcgc cagagcgcca cctgctcatc gtagacctgc atcatatcat tacggatggg
      721 atctccaagg gggtgctcat caatgatttt atcgctatct atcaggatga accgctgccg
      781 ccaccggccc ttcactacaa ggattttgcc gaatggcagc agggacccgg gcaacgccgg
      841 ctgtcggccg tgcaacgcga gttctggctg gagcaattct cgggagagat accggtgctg
      901 gaattgccgg cagattttcc acgtcccccg gtaaaagatt atgcgggcgg gcgtttcatg
      961 ttcgccctcg gcagggaaga cacggccgcg cttaagtccc tggcccttgg ggaaagggtg
     1021 acgctttatt cggtgatcct tgcgctatac aatgtattgc tggccaggct cagcaaccag
     1081 gatgatatcg ttgtcggcac gccggtggcg ggccgtcctc atgcggacct cgagagggtg
     1141 atgggcatgt tcatcgatac gatacccgtg cgcaatcacc ctgcggaaaa cactggtttt
     1201 cgccaatttt tgcaggatct tcattccagg accaccgcgt gcctggataa tcaggcctac
     1261 ccgtatgaac aactggtgga agagctgaag gtcccgcgca ataccggtcg caatcctttg
     1321 ttcgacgccc ttttcatctt ccacaatttc gagatgttcc tgctggagat gcgcggcctg
     1381 gtcatacggc ggatggagcg ttctcatccc atctcgaaat tcgatctgac gcttgaggcg
     1441 gtggaatcgc cggagggttt gtcctgcacg ttcgaatatt cgacaaacct gttccgtcgg
     1501 gatacggtgg agcgatttgc gggatacttc cagcggatag tcgccgcggt gacggaggac
     1561 ccggaggtga agcttggggc gatcga
//
LOCUS       DASYSV010000058         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_397109, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000058 DASYSV010000000
VERSION     DASYSV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397109"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VGQ51_01725"
     CDS             complement(<1..323)
                     /locus_tag="VGQ51_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035302.1"
                     /translation="MTPRSLQFICASTVILLSAALPSGAQTASWPKTINTAGGTIIHL
                     YSPQILSWSGDSVQSRSVISVQGAPDREPVFGVAWTTARVVADADNKLLTIGVVRVDR
                     LSIPG"
     gene            complement(435..1085)
                     /locus_tag="VGQ51_01730"
     CDS             complement(435..1085)
                     /locus_tag="VGQ51_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEV9035303.1"
                     /translation="MVKLGSTFYDRPGVVRVARALLGKVLVTEFDGERTSGRIVEVEA
                     YNGVVDRASHAWSGRRTKRTEVMFGAGGSAYVYLIYGIHHLFNVVTNRKDVPHAVLVR
                     GLEPLDGIPVMLQRTGKVRLDLSLTRGPGNLSKAMGLLTVHTGLSLMGKEVWIGDDGF
                     RVRPSEIGISARIGVDYAGEDALLPYRFFIKGNPYVSGKKGLNAKDDGDTRRLKSD"
     gene            1124..1888
                     /locus_tag="VGQ51_01735"
     CDS             1124..1888
                     /locus_tag="VGQ51_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEV9035304.1"
                     /translation="MLKEIIIAFQSYLKAHQFISKHRIWKWILIPGILYTILFCIGMY
                     FFWASATTAVATLSRFIGIERFLQHQHSAGLSFLFVMGGIMVHLVLVFFYFSLFKYLF
                     LIIGSPLFAYLSQLTEALINGKEDDFKLDGKQMLQDMRRGIRVALRNGLWQTVYTISI
                     LILSFIPLIGWITPVISTFVECYYYGFSMLDYSCQRHKLSQSASLAYISRHKGLAIGN
                     GIIFYGMHLIPVLGWVLAPAYAVIAATISLYHQNLE"
     gene            1890..2591
                     /locus_tag="VGQ51_01740"
     CDS             1890..2591
                     /locus_tag="VGQ51_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV9035305.1"
                     /translation="MVYLIPCWLEDHIVTPLPAYISDAVKDCQTFFVENERSARRYLK
                     ALWPEMIIDNYEWHPIHKLEETPEITAAFRKALAGNATIGILSEAGCPGIADPGQILI
                     AIAQQSGAIVRPLVGPSSILLALMGSGLNGQQFRFTGYLPVDNAGRTKALKDLETDSQ
                     RHHCTQLFIETPYRNNQLLATVLQTCRPATRLCIAANLTGPAEYIRTLAISEWRQLTP
                     LPDLNKQPAVFCLLA"
BASE COUNT          604 a          956 c          578 g          603 t
ORIGIN      
        1 tcaccgggga tggaaagcct gtctaccctc accaccccga tggtcagcag cttattatcc
       61 gcatccgcga ccacccttgc agtcgtccat gccacgccga agaccggctc gcgatccggc
      121 gcaccctgca ccgatatcac actgcgggat tgaacgctgt ccccggacca ggacaatatt
      181 tgtggtgaat atagatggat aatggtacca ccggcggtgt tgatggtttt cggccagctg
      241 gcagtctgcg cgccggaagg gagcgctgcc gacagtaaga tcaccgtcga cgcacagata
      301 aattgtaatg atcggggtgt cataaagtgt ttttgatagg tgagagcaac ggaggtcagc
      361 ctgatcctaa gttactactt agtaaccccg aaacctacac cccgcctcat ctttaatcca
      421 actttcatcc ggctttaatc gctctttaac cgacgcgtat ctccgtcgtc tttcgcattt
      481 aatcccttct tcccgctaac atacgggttc cctttaataa agaagcgata cggcagcaac
      541 gcatcttccc cggcataatc caccccgatc ctcgccgaga tgccgatctc cgacggccgc
      601 acccgaaacc cgtcatcgcc gatccatacc tcctttccca tcagcgacaa tcctgtgtgc
      661 accgtcagaa gccccattgc cttcgacaga ttgcccggtc cgcgcgtcaa agaaagatcc
      721 aaccgcacct ttcctgtacg ctgaagcatc accggtatac cgtccagcgg ctccagcccc
      781 cggacgagca ccgcatgcgg cacatctttc ctgttcgtca ccacattgaa gagatgatgg
      841 atgccataga tcaggtatac atatgcgcta cctcccgcgc caaacattac ctccgtacgt
      901 ttggtcctgc gtcccgacca ggcatggctc gcgcgatcca cgacgccgtt ataggcctcc
      961 acctctacga tccgcccgct ggttcgctcg ccatcgaatt ccgtgacgag caccttgccc
     1021 aaaagcgccc gcgccaccct taccacaccc ggtcgatcat agaatgtgct tcccagtttc
     1081 accatattta cgtaggtttg acgaatatca aaggtagtcg cacttgctga aagaaatcat
     1141 tatagccttt caatcttatc tgaaagcaca ccaattcatt tccaaacacc gcatctggaa
     1201 atggatcctg atacccggca tcctgtatac gatcctgttt tgtatcggca tgtacttctt
     1261 ctgggcctcc gcaaccaccg ccgtcgccac cctcagccgt ttcatcggca tcgaacgctt
     1321 ccttcaacac cagcatagcg cgggcctcag cttcctgttc gtcatgggcg gcatcatggt
     1381 ccacctcgta ctcgtgttct tctatttctc gttgttcaaa tatctcttcc tgatcatcgg
     1441 ctcaccttta ttcgcctatc tcagccaatt gaccgaggcc ctcatcaatg gcaaggaaga
     1501 cgatttcaaa ctggatggaa aacaaatgct gcaggatatg cgccgcggca tccgcgtagc
     1561 cctccgaaat gggttatggc agacggtcta taccatttca attctcatcc tgtcctttat
     1621 cccgctgata ggctggatca cgcccgtcat ctccacgttc gtggaatgtt attactatgg
     1681 cttctcgatg cttgactaca gctgtcagcg gcacaaactc tcccagtccg ccagcctcgc
     1741 ctatataagc cgccacaagg gcctcgccat aggcaacggc attatctttt atggtatgca
     1801 cctgataccc gtgctgggct gggtgctcgc ccccgcctat gccgtcatcg ctgcgactat
     1861 cagcctctat caccagaatc ttgagtaaaa tggtatacct catcccatgc tggctggaag
     1921 accacatcgt tacgcctctc cccgcgtaca tcagcgacgc cgtcaaagac tgtcaaacct
     1981 ttttcgtgga aaacgaacgt agcgcccgcc gctaccttaa agccctatgg ccggaaatga
     2041 tcatcgacaa ctatgaatgg catcctatcc acaagctcga ggaaaccccc gaaataaccg
     2101 ccgccttccg caaagcgctc gccggcaacg ccaccatcgg catcctctcc gaagccggct
     2161 gccccggcat cgccgacccc ggccagatcc tcatcgccat cgcccagcaa tccggcgcca
     2221 tcgtccgtcc cctcgtagga ccgagctcga tcctgctcgc cctcatgggc agcggtctca
     2281 acggtcagca gttccgcttc accggctatc tccccgtcga caatgccggc cgaacaaagg
     2341 cgctcaaaga cctcgagaca gattcacaac gccaccactg cacccagcta tttatcgaaa
     2401 cgccttatcg caataaccag cttctcgcta ccgtcctcca aacctgtcgc cccgcgacac
     2461 gtctttgcat agccgccaat ctcaccggcc ccgccgaata catccgcacg ctagctatct
     2521 ccgaatggcg ccagctcaca cccctccccg atctcaataa acaacccgcc gtcttctgcc
     2581 tcctcgcctg accgccccgg cagccagccg cacctcccaa accaccaccc cgcagcccgc
     2641 cgcccccggc aactcccccc atctacttgg tcacctttcc cgactccgca tccagacgaa
     2701 atatctgcag tttcctatag atcggcgaat tcacggtagc c
//
LOCUS       DASYSV010000059         4395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1678561, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000059 DASYSV010000000
VERSION     DASYSV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678561"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..3691
                     /gene="treS"
                     /locus_tag="VGQ51_01745"
     CDS             236..3691
                     /gene="treS"
                     /locus_tag="VGQ51_01745"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEV9035306.1"
                     /translation="MADPTALDDELHWYKDAIIYELHIKAFCDGDADGVGDFQGLLQK
                     LDYLQDLGVTAIWLLPFYPSPLRDDGYDIADYYSINPSYGDISQFKHLLKEAHQRNLR
                     VITELVINHTSDAHAWFQRARQAPKGSKERNFYVWTDDPGQYKDVRIIFQDFENSNWT
                     WDPVAEQYYWHRFFHHQPDLNYDNPAVQDEVFRIIDNWCKMGVDGFRLDAVPYLFERE
                     GTNGENLPETHDFLKKLRRHIDERFPGTVLLAEANMWPEDSASYFGNGDECHMNYHFP
                     VMPRMFMALQMEDHYPITDIFDQTPEIPATCQWAIFLRNHDELTLEMVTNEERDYMYK
                     VYARDPKARINLGIRHRLAPLLDNNRRKIELLNSLLFTLPGTPVIYYGDEIGMGDNFY
                     LGDRDGVRTPMQWSPDRNAGFSAANPQKLYLPLILDPEYHYESVNVETQQSNTSSLYW
                     FMKRIIAVRKKSRTFGRGNLKFVSVENPKVLSFTRTYEDETLLIVVNLSKFSQAAEVD
                     LVAYKGFVPVDVFSRNRFPVIREDGQYFFTLAPYAFNWFVLEKAHPEMDEEKPLPLLL
                     LRKWSGLMEGTARKQLESTILPRYLVRAKWFIPKERYTYQVVVTDYAVLSADDRSVFW
                     LLLEITGDDGITGLYQLPLWVVREEAAKKLGEVFPEALVAQVKSGEGDGILVDAFYAP
                     EWQLVLLQKLSGQKQGHPAAGPIIYETNPRVKQYLQQAEGLVPRIHSSDFYNTSIQYD
                     GFFYLKLYRSVTWSVHPDQELTRWLSEESKFPYLPAFIGSIEWKDKKGCVVLGLLQEM
                     IENHGDGYGYFQERINNYLERLLALEKNGPLPDRQIGTMEEPIAFEELPPEMQNLLGV
                     HAADLARLIGTRTAEMHLALAAATGRDMKPEPFSLHYQRSLYSSITSLVRETYENLSR
                     RIQSLPKGWHERMDRVLAYRPELLTTLRKIYTRKMEVSKIRIHGNYHLGQVLLTGKDL
                     AISDFSGDPSLSYSERRLRRSIFVDLASMIASFHDVAFEGFLHGRQVHAEDAQRLLPM
                     ATFWAHYISGFFIRAYKEKAAGSVLIPSAPADFSALLQYFVVQKAMTVFNVYLKKDPR
                     KLIIPQTMVRAVLPAQQESVAVSAPVVPEVGGQTLKEKPAMSTVQSEEIATPKPADVA
                     AESKK"
     gene            complement(3680..>4395)
                     /locus_tag="VGQ51_01750"
     CDS             complement(3680..>4395)
                     /locus_tag="VGQ51_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV9035307.1"
                     /translation="SQRYDLPVYISRPTLRECRFAPAPQLVHHFDASQPVTIGDLTVT
                     AFSKAHDASDPYSFTIASPSVCIGVFTDIGAVCGNVIRHFTQCHAAFLEANYDEQLLE
                     KGRYPWPLKNRIRGGHGHLSNRQALELFMDYRPAFMSHLLLAHLSQDNNRPELVQSLF
                     DSHAAGTTISIASRYNESEIYPIDGRYSGVAGPAWTFDGQPKPAAPVMPVAAAAGERN
                     TASRPGRPATPAPIQISLF"
BASE COUNT         1026 a         1087 c         1313 g          969 t
ORIGIN      
        1 ccaggacggt gaagtagggc gccagcatta cccgctgacc tgaggaaaga gggggagagg
       61 ggggcattcc accggcatta ccagggacta cgaacaacaa gacgaaaagc agccaaactg
      121 ctgattttaa ggaccgttca gggatcatcc gaataaaaat acaaaaattc agcagatagg
      181 gggatcaaat tttttgttga gtggggtcgg gcacaggatt tgaaacactg tccgtatggc
      241 agatcctacc gcactcgatg atgaattgca ctggtacaaa gatgcgatca tctatgaatt
      301 acatatcaag gccttttgtg acggtgatgc cgatggcgtc ggggatttcc agggattgct
      361 gcagaaactg gattatctgc aggacctggg tgtgaccgcc atatggcttt tgccgtttta
      421 tccctcgccg ttgagggatg acggatacga tattgcggac tattacagca tcaatccttc
      481 ctatggcgat atttcccagt tcaagcattt attaaaagag gcccatcaac gcaatctgcg
      541 ggtgataacc gaactggtca tcaatcatac atcggatgcg catgcctggt tccagcgggc
      601 ccggcaagcg ccgaaaggtt cgaaggagcg gaatttttat gtctggacgg atgatcccgg
      661 ccaatacaag gacgttcgca tcatcttcca ggatttcgaa aattcgaact ggacctggga
      721 tccagtggcg gagcaatatt actggcaccg attttttcac caccagccgg acctcaacta
      781 tgacaacccg gcggtgcagg atgaggtttt ccggatcatc gataactggt gcaagatggg
      841 ggtggacggg ttccggctgg atgccgtgcc ttatttgttc gaacgggaag gcacgaatgg
      901 cgagaacctt ccggagacgc atgatttcct gaagaagctg cgccggcata tcgacgagcg
      961 atttcccggg acggtcctcc ttgcggaggc gaacatgtgg ccggaagatt ccgcttccta
     1021 tttcggcaat ggcgatgaat gtcacatgaa ctatcatttt cctgtgatgc cgcgaatgtt
     1081 catggcgctg cagatggaag accattaccc tatcaccgac atctttgatc agacgccgga
     1141 aattcccgcg acctgtcagt gggccatttt tcttcgtaac catgacgagc tgacgctgga
     1201 gatggtgacc aatgaggaaa gggattatat gtataaagta tatgcccgtg atcccaaggc
     1261 gcggatcaat ctgggcattc gtcaccgtct ggcccctctg ctcgataaca accgccggaa
     1321 gatcgaattg ttgaattccc tattgttcac attgccgggg acccccgtca tttattacgg
     1381 cgatgagatc gggatgggtg acaatttcta cctcggagac cgcgacgggg tacgcacgcc
     1441 gatgcaatgg tctccggacc ggaatgcggg cttttcggcc gccaacccgc aaaagctgta
     1501 tctgccactc atactcgacc cggaatacca ttatgaatcg gtgaatgtgg aaacacagca
     1561 aagcaatacg tcttccctgt actggttcat gaagcggatc atcgccgttc gcaagaagtc
     1621 gaggacattc ggacgcggca atctgaaatt cgtaagtgta gagaacccga aggtgctgag
     1681 ctttacgcgt acctatgagg acgagacctt gctgatcgtc gtcaacctgt ccaaattctc
     1741 gcaggcggcc gaggtcgatc tggtggcata caaaggattc gttccggtgg atgttttcag
     1801 caggaacagg tttcccgtga tcagagaaga cggacagtat ttcttcacgc tggcgccata
     1861 tgccttcaac tggttcgtgc tcgagaaggc tcatcccgaa atggatgagg aaaagccatt
     1921 gccactcctg ctgctgcgca aatggtcagg actgatggaa gggacggcga gaaagcaatt
     1981 ggagagtacc atcctgccgc gttacctggt gcgcgcgaag tggtttattc cgaaggagcg
     2041 atatacctac caggtggtag tcaccgacta tgccgttctg tctgccgacg atcgttccgt
     2101 gttttggctg ttgctggaga tcaccggaga tgacggtatc acggggttat accagttgcc
     2161 gctatgggtg gttcgggagg aggctgcaaa aaagttgggc gaagtcttcc cggaggcgtt
     2221 ggtcgcgcag gtgaagtccg gggaagggga cgggatactg gtcgacgcct tctatgcccc
     2281 ggaatggcag ctggtcctgt tgcagaagct gtcgggacag aaacagggac atcctgctgc
     2341 gggtcccatc atctatgaga ccaatccacg cgtcaaacaa tacctgcaac aggcagaggg
     2401 tctcgtgccg aggatacaca gctcggattt ttataatacc tctattcaat atgacgggtt
     2461 cttctacctg aaattatacc gttccgtgac ctggtcggtc catccggacc aggagctgac
     2521 ccgctggctt tcggaggagt cgaaatttcc ctatctgccg gcattcatcg gcagcatcga
     2581 atggaaggat aagaagggtt gtgtcgtgct cggcttatta caggaaatga tcgagaatca
     2641 cggtgacgga tatggatatt tccaggagag gatcaacaac tatctcgaac ggctattggc
     2701 gctggaaaag aacggtccgt tgccggaccg gcagataggg acgatggagg aacctatcgc
     2761 attcgaagag ctgcctcccg agatgcagaa ccttctgggc gtccatgcgg cagacctggc
     2821 gcggctgatc gggacccgga cggctgaaat gcatctggct ctcgccgccg cgacaggcag
     2881 ggatatgaag ccggaaccct tttcgctgca ttatcagcgc tcgttgtatt cttccatcac
     2941 ctcgctggtg cgggagacct atgagaatct ttcgcgtcgt attcaatcgc taccgaaagg
     3001 atggcatgaa cggatggacc gggtcctggc ctatcgtccg gagttgctga ccaccctgcg
     3061 aaagatatac acccgtaaaa tggaggtctc gaagatccgc atccacggaa attatcatct
     3121 ggggcaggtg ctgttgacgg gaaaggacct tgccatcagc gatttcagcg gagatccctc
     3181 actgagctac agcgagcggc ggctccggcg aagcatattc gtcgatctgg cttcgatgat
     3241 cgccagcttt catgacgtgg cattcgaagg gttcctccat ggacggcagg tccatgccga
     3301 agatgcgcaa cgattgttgc cgatggcgac cttctgggct cattatatca gcggattctt
     3361 catacgcgct tataaggaaa aggcggcggg ttcggtgctt ataccgtctg cgccggcgga
     3421 tttcagcgcc ctgttgcaat atttcgtagt gcagaaggcg atgacggtct tcaatgtcta
     3481 tctgaaaaag gatcccagaa agctcatcat tcctcagacg atggtgcggg cggtgttgcc
     3541 ggcgcagcag gaatccgtcg ctgtatcggc accggtggtt ccggaggtcg gtgggcaaac
     3601 gctgaaagag aagccggcga tgagcaccgt tcaatccgag gagattgcga cacccaagcc
     3661 tgcggacgtg gctgcggaat caaaaaagtg atatttgaat gggggcgggt gttgcgggac
     3721 gcccgggacg ggaggcggta tttcgctccc cggccgccgc agcgacgggc atgacagggg
     3781 ctgcaggctt tggctggcca tcgaatgtcc aggcgggtcc ggcgacgccg ctgtaccggc
     3841 catcgatggg atagatctcc gattcattat acctcgaggc gatgctgatg gtagtacccg
     3901 ccgcgtggct atcgaaaagg gattgtacaa gttcgggccg gttgttatcc tgcgacagat
     3961 gcgcgaggag gagatggctc atgaatgccg ggcgatagtc catgaaaagc tccagcgcct
     4021 gccggttgga aagatggcca tgaccgccgc gtatgcgatt cttcagcggc caggggtagc
     4081 gtcctttttc aagcagctgt tcatcataat tggcttccag gaaggcggca tgacattgcg
     4141 taaaatgacg gatgacattc ccgcagaccg cgccgatgtc ggtaaagacc ccgatacaca
     4201 ccgatggaga agcgatggta aaactgtaag gatcggaggc gtcgtgcgct ttcgaaaagg
     4261 cggtgactgt cagatcgccg atggtcaccg gctggctggc atcgaaatgg tgcaccagtt
     4321 gaggggcggg ggcaaaccgg cattcgcgca gggttgggcg ggagatatat acagggagat
     4381 cgtagcgttg ggaca
//
LOCUS       DASYSV010000060        11402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_527398, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000060 DASYSV010000000
VERSION     DASYSV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11402
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527398"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VGQ51_01755"
     CDS             complement(<1..1028)
                     /locus_tag="VGQ51_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEV9035308.1"
                     /translation="MNRIIKKVAVLGSGVMGSRIACHFAGVGIQTLLLDIAPKELTPA
                     ETAKKLTPEHPAVRNRIVNEALAAALKSNPSPIYTKEVVSRITTGNFTDNMKDIADAD
                     WVIEVVVERLDIKQQVFTEVEKYRRPGTLITSNTSGIPINLMAEGRSEDFKKHFCGTH
                     FFNPPRYLRLLEIIPTPMTDADVVGFLLHYGDLYLGKTTVLCKDTPAFIANRVGVYGI
                     MAIFGLVEQLGLTVDEVDALTGPLIGRPKSATFRTADVVGIDTLVKVANGVAENCPDD
                     EQRSVFTIPAWLDKMVANKWLGDKTGQGFFKKTKGAGGEKEILTLNLNTMEYGLRQRP
                     KFPSVEAA"
     gene            complement(1123..1761)
                     /locus_tag="VGQ51_01760"
     CDS             complement(1123..1761)
                     /locus_tag="VGQ51_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035309.1"
                     /translation="MKQNYSYRILPYNRYSSLYQERESFYRSFINQYYLDFAHQVLTQ
                     EHYSPYLTKTLIAPQDIQFGASPRRVIWKIGMPSFQVKDINDFKDHKILFYRTKLLRQ
                     KVLIQFHFINSFFYYSQLSFLTFTEKTNNILFDSLRSKYECPESLEEQDKACMTDICG
                     NRLIIEKGIYLTVSYITGDSYPREQMKVQLAQKTELVNRADNYEIDKLSKIL"
     gene            complement(2012..4132)
                     /gene="metG"
                     /locus_tag="VGQ51_01765"
     CDS             complement(2012..4132)
                     /gene="metG"
                     /locus_tag="VGQ51_01765"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV9035310.1"
                     /translation="MHQPKRYLITAALPYANGPKHIGHLAGAYLPADIYVRYLRAQRR
                     DVAFICGSDEHGTAIPIQAMKEGTTPRAIVDKYHALLKENFAELGISFDIYHRTTDPI
                     HHVTAQEFFTHLNDRDELIVQESEQYYDEEAKTFLADRYIVGTCPVCRNPRAYGDQCE
                     NCGTSLSPDMLIEPRSTLSGNPPVKKKTRHWYLPLDRHEAFLRQWILGEHEGDWRANV
                     VGQCKSWLDGGLQPRAVTRDLDWGVKVPLSEAEGKVLYVWFDAPIGYISATKQWSLDT
                     GKDWEPYWYDKETKLVHFIGKDNIVFHCIIFPVMLRLHGNILPENVPANEFMNLEGDK
                     MSTSRNWKLDMQDYILDFIKPANGGTQLADSLRYYLTAISPETKDSEFTWKGFQDANN
                     SELVAKFGNFVNRTFVLMHKLCNGKVPGLHKTRMDDADEEMIAAIGATRTKVEQLLEN
                     YRFREAQFEVIMLSDLANKYMQKKEPWIVVRSLAQDSANQQLIDNCLHICLQLTANLA
                     ILINPFLPYTAKKMLHMMKVVEKMLDWENAGKLNLLSVGYSLRAPELLFRKIEDEEVN
                     IQMEKLRTASAARVGNTAVAPTTPAAEKPETKPTIVYDDFAKMDLRVGTIISAEKVEK
                     ADKLLKLGIDLGYEQRTIVSGIAQHFKPEEIVGRRVVVVANLAPRKMRGIESNGMILM
                     AEDGAGGLHFVHAGEQAEPGAAVS"
     gene            complement(4182..5057)
                     /locus_tag="VGQ51_01770"
     CDS             complement(4182..5057)
                     /locus_tag="VGQ51_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035311.1"
                     /translation="MRLLIILLMISGFIGEAKGQDLTGQWTGTATDKTSESKQKLVLS
                     ITEGDSAFGGVLHWYSPETRTIRHIIVSGRFYGKDSILTIREDSTRQNVVGYASGGDP
                     AAKGPPGGFYVLFYRRTAGRKDMLEGHWQANFQSVMKTGEWTIRMEKKAPAFIPVPMA
                     KLRKKDSADVRQFQALQSRESVVAARIPAQGIDTIRVALYDNGEIDGDSVSLYINNEL
                     VAQHVRLSADAKVILVPIDKSIAVNKLVLFAENLGRLPPNTALMEITVHGQTYNLFLS
                     TDYKRNASVEFLLEE"
     gene            5140..6219
                     /locus_tag="VGQ51_01775"
     CDS             5140..6219
                     /locus_tag="VGQ51_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV9035312.1"
                     /translation="MNRKNFLSSLLAIGATGPVLAANRTTAAHGTAAAAHNITPPLII
                     PPYLRPGDTIGITCPAGDITAKDIEPAVQLIESWGFRIKVGDTVGKKDFIFGGTDEER
                     AADFQQMLRDPGLSAILCARGGYGAVHIVDQLDFSSLAQRPKWIIGFSDITVFLCHLH
                     TNLNLASIHSKMCNSFPDDWSKAEPVQADSILSIRRALSGEKMEYSATPHPMNRSGFA
                     EAPLVGGNLKTIESLAGTVSDIDTAGKILFVEDTGEYLYSIDRMFWHLQRTGKLDRLA
                     GLVVGGFKVKPDDPGDEYGHTVYEIVSARMKPYSYPVCFDFPVGHQKDNYALKYGMPH
                     MLEVKTDSVTLHERAVSGLQTTTPA"
     gene            6216..7124
                     /locus_tag="VGQ51_01780"
     CDS             6216..7124
                     /locus_tag="VGQ51_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV9035313.1"
                     /translation="MITIPPFLRPGDTIGITCPAGFMTREKALTCIGTLETWGYRVRE
                     GRTLGGDSDNYFSGTDEQRLDDLHAMLADPGIDAILCGRGGYGLSRIIDRVDFSAFCQ
                     KPKWIIGFSDITVLHAHLFTNYKIATLHAPMAGAFNDGCADTPGVLSLKRVLAGEPMS
                     YTCAPHPFNRTGYTRGILVGGNLSLLAHLVGSVSDIDTKGKILFLEDVGEYLYNIDRM
                     LRQLKRSGKLDHLAGLVIGGFTETKDTMRPFGATVEDIIREVVKEYAYPVCYGFPVSH
                     GIENVALKIGTEYTLTVAPGSTALTE"
     gene            complement(7114..7227)
                     /locus_tag="VGQ51_01785"
     CDS             complement(7114..7227)
                     /locus_tag="VGQ51_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HEV9035314.1"
                     /translation="MGAVEQDINGDTLMVYNNTYYLPISQDGQDAYEVVTP"
     gene            7430..8260
                     /locus_tag="VGQ51_01790"
     CDS             7430..8260
                     /locus_tag="VGQ51_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035315.1"
                     /translation="MKKWLSFLLLPAVLWVASCKKSGSSNKNSNNNAWLSSVVSLQPQ
                     TRVVDSFYYDSSHRITGFHQYEYDSSQGYPAFGEWTVQFAYPAGTASQPSSYLYVAGF
                     SPDIHALTYDAQGRITRDTSVSGTGYVAHYSYPNGNIATTVLFDGTNANNQIDTMFLS
                     NGNVSTLRIYYPNTAGTADSLEGAVNFGFTSLANPAYHADITNSIGPLLYILQLDGFG
                     SSVDPISQHALNSVGGSGSGLPPGVTLKYNQTTDSQGRLIELSSPLGPVAGSISFTYY
                     "
     gene            8266..9201
                     /locus_tag="VGQ51_01795"
     CDS             8266..9201
                     /locus_tag="VGQ51_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEV9035316.1"
                     /translation="MTFVQLEYIVAVDTYRHFAIAAGHCFVTQPTLSMQVHKLEEELG
                     LKIFDRSKQPVIPTEAGREIIEQARRILAEKEVIGEIVQTKKGILTGELRIGIIPTLA
                     PYLLPLFVQSFTAKYPQVKLVVNELMTDLVVSRLREGRIDAGILVTPLQENGIREQVL
                     FYEELLVYVSRKNKAYKKTYMLARDIDPGKLWLLEEGHCFRSQIVRLCELRKASKEGS
                     HFDYEAGSLETLRRMVELNDGITILPELAVRDLTGKQQQLIRHFRRPAPMREVSLVTH
                     RDFVKQRLVQTLYQEILRAVPEKVRQNKNQRVVPI"
     gene            complement(9180..9893)
                     /locus_tag="VGQ51_01800"
     CDS             complement(9180..9893)
                     /locus_tag="VGQ51_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV9035317.1"
                     /translation="MRKLFFRWLKVVVLVYCLIGIGIYYAQDRLVMHPVTLPATQNFA
                     FDRPFTEVHLPYDAQTNLDVVEFKASPADSVPEGVVLYFPDSKGNIATNAQDALGFAP
                     RGYEVWVMDYPGFGKSTGVFTEKNVYAYALIFYKLARSRWKPSQIVICGTGLGTGIAA
                     QLASVRNCRRLILNDPYYSMTSRFRKWLFLYPVGLMLHYHFPTYQYLPAVTDPITMIG
                     GDTRLKAFLKPGDQMGTTR"
     gene            complement(9903..10196)
                     /locus_tag="VGQ51_01805"
     CDS             complement(9903..10196)
                     /locus_tag="VGQ51_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035318.1"
                     /translation="MKKIIVLFLPVIFFAVTAMSQTAGQVPIKQSDATPRLTPKTISI
                     PIKPAGPVGATAQKLTAENMTGTTQATKTATTVPAKNTGTLPSQTPVKVPQKQ"
     gene            complement(10204..>11402)
                     /locus_tag="VGQ51_01810"
     CDS             complement(10204..>11402)
                     /locus_tag="VGQ51_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035319.1"
                     /translation="NSNTNNIANTLNVTTNTQGDIPDHSFGNAGNFFNNNNFGVGAGV
                     RGEVNSIFGNNGTAGVYGTSSGTGGYGGYFEHSNTAGFGYAMEAITYGLGVGADFQTQ
                     NANNTQPTLQAQTIGINPAGYYVNYNANNPSNVLSVTTNSNGSISDHSTANASNFFVN
                     NNFAVAAAVRGEVNSIFGNEGTAGIYGISSGTGGYAGFFEHTETTGFGITLQVVSNDQ
                     GVAEVVDQEGPNDILWFQSHGGNKARIDNTGRGYFDGGTQNSGADLAEVFDVEGKFGD
                     YEPGDVLVISEVKDRTLVRSAAAYSMLVAGVYATKPGVLLTEESLNGSIAGKVPLGVV
                     GVIPTKVCDEGGPIHRGDILVTSSRAGYAMKADLHRLQPGQAIGKALENFEGPAGKIK
                     VLVNVK"
BASE COUNT         2551 a         3341 c         2800 g         2710 t
ORIGIN      
        1 ctggccgctt ccaccgaagg aaatttcgga cgttgtcgca gaccgtactc catcgtattt
       61 aaattcaagg taaggatctc cttttccccg ccggctccct tcgttttctt gaaaaaaccc
      121 tgtcccgtct tatcccccag ccatttgttc gcgaccatct tatccaacca ggcagggatt
      181 gtgaagacgc tccgctgttc atcatcggga caattctccg ccacgccatt cgccactttc
      241 accagtgtgt cgatgccgac cacatccgcc gtgcgaaagg tggctgactt cggcctcccg
      301 atcagcggac ccgtcaatgc atcgacctca tctaccgtca gcccgagttg ctccaccagc
      361 ccgaatatcg ccataatgcc atagacgccg acgcggttgg caatgaatgc cggggtatcc
      421 ttgcaaagca ccgtcgtctt gcccagataa agatcgccgt aatgcagcag gaagccgacc
      481 acgtcggcat cggtcatggg cgtggggatg atctcgagta atcgtaaata acgaggtgga
      541 ttaaaaaaat gcgtcccgca gaaatgtttt ttgaaatcct cgctccgtcc ttcggccatc
      601 aggttgatgg gaatgcccga agtattcgag gtgatcagcg ttcccggcct gcggtatttc
      661 tccacctctg taaatacttg ctgctttata tccagccgct ctaccaccac ttcgatcacc
      721 caatcggcgt cggcgatatc cttcatgttg tcggtaaaat tgccggtcgt gatccgtgac
      781 acaacttctt tggtatatat tggagagggg ttggacttga gtgccgccgc aagggcttcg
      841 ttgacgatcc tgttccgcac ggcaggatgc tccggggtaa gctttttggc cgtttctgca
      901 ggggtcagct ccttcggtgc gatgtccagc aaaagggtct gtatcccgac gcccgcaaaa
      961 tgacaggcga tccgtgaccc cattaccccg cttcccagaa cggccacttt cttgatgata
     1021 cggttcatat ggttaattag ttagttaaac aactattcta gccgaaggta ggaaaaaaaa
     1081 ggattggccg cgcatgcggc gggacccggc gggatttctt gtctaaagga tcttgcttaa
     1141 tttgtcgatc tcgtagttgt cggccctgtt gaccagttcg gtcttttgcg ccagctgcac
     1201 cttcatctgt tcacgagggt aggagtcgcc cgtgatatag ctgacagtaa ggtatatacc
     1261 cttctcgatg atcagcctgt tgccacaaat gtcggtcata catgccttat cctgttcttc
     1321 gagagattcg gggcattcgt atttgctccg cagcgagtcg aaaaggatgt tgttggtctt
     1381 ttccgtaaag gtcaggaagc tgagctgtga gtagtagaaa aagctgttga tgaaatggaa
     1441 ctgtatcagc accttctggc ggagaagttt ggtgcgatag aaaagtatct tgtggtcctt
     1501 aaagtcattg atgtccttca cctggaaaga aggcatccct attttccaga tgacacgacg
     1561 cggagatgcg ccgaactgga tgtcctgcgg cgcgatcagg gtcttggtca ggtaaggcga
     1621 atagtgctcc tgagtcaata cctggtgtgc aaagtcgaga taatactggt tgatgaagct
     1681 gcgatagaaa ctctctcttt cctggtataa agaactataa cggttatagg ggaggatccg
     1741 ataagaatag ttctgtttca tagcacagtg gattgcgtta gattgatatt agattttttt
     1801 tgtattcgta atatcttgct aaatacatga atgatcgccc attttccaca aatgaaaaat
     1861 cattcgaata aaaacgctga cattcaatgc aaagtattct gccgcaaaaa tttaagcggt
     1921 tacccctaag aatatgtcct acttcgcact acagctaccc atttccacta tactatccca
     1981 agggctctcc agcttctatt cagattcatg atcagctgac cgccgcccct ggttcggcct
     2041 gctccccggc atgcacgaaa tgaaggcctc ccgcaccatc ttcagccatc aggatcatcc
     2101 cattactctc gataccccgc attttcctcg gagccaggtt ggcgacaacg acaaccctcc
     2161 ggccgacgat ctcctccggt ttgaaatgct gcgcgatacc ggaaacgata gtccgctgtt
     2221 cataaccaag atcgatgcca agtttcagca atttatcggc cttttccact ttctcggcgc
     2281 tgatgatggt gcccacgcgg agatccatct tcgcaaaatc atcgtatacg atggtaggct
     2341 tggtctccgg cttctccgcc gccggtgtag ttggtgccac cgccgtattc cccacgcgcg
     2401 ctgccgaggc agtacgcagc ttttccatct gtatattcac ttcttcatcc tctatcttcc
     2461 tgaacaaaag ttccggcgcc cgcaggctat accctacgct caggagattc agcttccctg
     2521 cattctccca atcgagcatc ttctccacca ccttcatcat atgcagcatc ttcttcgccg
     2581 tgtaaggcaa aaaaggattg atcagtatcg ccagattcgc cgtcagctgc aaacagatat
     2641 gcagacagtt atcgatcagc tgttgattgg ctgagtcctg cgccagactt ctcaccacga
     2701 tccagggttc ttttttctgc atatacttgt tggccaggtc ggataacatg atcacttcga
     2761 actgcgcttc gcgaaaccgg taattctcca gcagctgctc caccttcgtc ctggtcgcgc
     2821 caatagcggc gatcatttcc tcgtcagcat catccatccg cgttttatgc aagcccggta
     2881 cttttccgtt acacagcttg tgcatcagca cgaaggtgcg gttgacgaaa ttgccgaatt
     2941 tcgctaccag ctcgctgtta ttcgcgtcct ggaaaccttt ccaggtgaat tcgctgtcct
     3001 tggtctcggg cgagatggcc gtcagataat agcgaaggct gtccgccagc tgcgttccgc
     3061 cgttggccgg tttgatgaaa tcaagaatat aatcctgcat gtccagcttc cagtttcgac
     3121 tggtgctcat cttgtctcct tccaggttca tgaattcgtt ggccgggaca ttttcgggca
     3181 ggatatttcc atgcaaccgc agcatcaccg ggaagatgat gcaatggaaa acgatattgt
     3241 ccttgccgat gaaatgcacc agcttggttt ctttgtcgta ccaatatggc tcccagtcct
     3301 tgccggtatc caggctccac tgcttcgtgg cgctgatata cccgatcggc gcgtcgaacc
     3361 agacatacag cacttttccc tctgcctcgg acaggggaac ctttacgccc cagtccagat
     3421 cgcgtgtgac ggcccggggc tgaagaccgc cgtcgagcca gctcttgcac tgtccgacga
     3481 cattggcccg ccagtcgccc tcatgttccc ccaggatcca ctggcgtaaa aaggcttcat
     3541 ggcggtccaa aggaagatac caatgcctgg tctttttctt taccggcgga ttaccgctca
     3601 aagtgctgcg cggttcgatc agcatatccg gacttaatga cgttccgcag ttttcgcatt
     3661 ggtcgccata tgccctcgga tttctgcata ccgggcaagt ccccacgata tacctgtccg
     3721 ccaggaacgt cttcgcttcc tcatcatagt attgctcgct ctcctgcacg atcagctcgt
     3781 cccggtcatt gagatgggta aagaattcct gggcggtgac atgatgaatg gggtcggtgg
     3841 tccgatgata gatatcgaac gagatgccga gttcggcaaa attctccttg agcagggcgt
     3901 ggtacttgtc gacgatggcc ctcggggtgg tcccttcctt catcgcctgt atcggtatag
     3961 ccgtcccgtg ttcgtcgctg ccgcagataa aagccacatc ccgccgctgc gcccgcagat
     4021 agcgtacata gatgtccgca ggcagatagg cgcccgccag atgaccgata tgtttcggtc
     4081 cgttggcata cggcaaggcc gccgtgatta agtatcgttt gggttgatgc atgtggacgc
     4141 aaaattacgc aaaatcaccc gcactgagca aacgtccgca ctcactcctc cagcaggaat
     4201 tctaccgacg cattccgttt ataatccgtg gaaagaaaaa gattataggt ttgaccgtga
     4261 accgttatct ccattaacgc cgtattcgga ggcagtctgc ccagattctc ggcgaataat
     4321 accaatttat tgaccgcgat gctcttatcg atgggcacca ggatcacttt cgcgtccgcc
     4381 gacagccgta catgctgagc caccagttca ttattgatat acaggctgac cgaatcgccg
     4441 tcgatctccc cgttatcgta caaggctacc cgtatggtat cgatgccctg tgccgggatg
     4501 cgcgcggcca cgacggattc acgcgactga agcgcctgga attgccggac gtcggccgaa
     4561 tccttcttcc tgagctttgc catgggcaca gggataaaag ccggggcttt cttttccatc
     4621 cggatcgtcc actcgccggt tttcatgacg ctctggaaat tagcctgcca atgcccttcc
     4681 agcatatctt tccgccccgc cgtcctgcga tagaacagca cataaaaccc tcccggtggg
     4741 ccctttgccg ccggatcgcc tccgctggca tatcccacga cattctgccg cgtgctgtcc
     4801 tcccgaatgg tcaggatgga atctttccca taaaaacgac cgctgacgat gatatgccgg
     4861 atggtccggg tctccggaga ataccagtga agcacacctc caaaggcaga atcgccctcc
     4921 gtaatggaca gtaccagctt ttgcttgctc tccgacgttt tgtccgtggc agtgccagtc
     4981 cattgtccgg ttagatcctg tcccttcgcc tccccaataa atcctgaaat catcaacagg
     5041 attatcaata aacgcatacg ctaaaagtag taattttaag cccgcgcctg aactcgttca
     5101 ggaaatcaga gcccgggcaa tattaccgta attgttaaca tgaaccgaaa aaactttcta
     5161 tcgtccctgc tggcgatcgg cgccacagga cccgtactcg cagctaaccg caccacggcc
     5221 gcacacggca cagctgcagc agcacacaac atcactccgc ctctcatcat cccgccctat
     5281 cttcgccccg gcgacaccat cggcatcacc tgccccgcag gcgacatcac cgccaaagac
     5341 atcgaaccgg ccgttcaact catcgaaagc tggggatttc gaatcaaagt cggcgatacc
     5401 gtgggaaaaa aagatttcat cttcggaggc accgacgaag aaagggccgc ggacttccag
     5461 caaatgctcc gggatccggg attgagtgcc atcctctgcg cacgcggtgg atatggcgcg
     5521 gtacatatcg tcgaccagct ggatttttca tcgcttgcac aacgccccaa atggatcatc
     5581 gggttcagcg atatcaccgt atttctttgt catctccata ccaatctcaa cctggcttct
     5641 attcactcca agatgtgcaa cagcttcccc gacgactggt cgaaagcaga acccgtccag
     5701 gcggattcga tcctctccat ccggcgggcg ctcagcggcg agaaaatgga atacagtgct
     5761 actccccatc cgatgaatcg ctccggtttc gccgaagccc cgctcgtggg cggcaacctc
     5821 aaaaccatcg agtccctggc cggcactgtc tccgatatcg acacggcggg caagatcctc
     5881 ttcgtcgaag ataccggtga atatctctac agcatcgacc gcatgttctg gcatctgcag
     5941 cgtacgggca aactggacag actcgccggc ctggtcgtcg gcggtttcaa ggtcaaaccc
     6001 gacgatcccg gtgatgaata cggtcatacc gtttacgaga tcgtctccgc caggatgaaa
     6061 ccctactcct acccggtgtg tttcgatttt ccggtcggtc accagaaaga caactatgcg
     6121 ttgaaatatg gtatgccgca tatgctcgaa gtaaagaccg acagtgtgac gctacacgaa
     6181 cgagcggttt caggcctcca aacgactacc cctgcatgat caccatccct ccctttctcc
     6241 ggcccggtga taccatcggc atcacctgtc ccgccggttt catgacgcgc gaaaaggcgc
     6301 tcacctgtat cgggacgctg gagacctggg gctatcgcgt ccgcgagggc cgaaccttgg
     6361 gcggcgattc ggacaactat ttctcgggca cagacgagca gcgtctcgac gatctgcacg
     6421 caatgcttgc agaccccggc atcgatgcca tcctctgcgg ccgcggcggc tatgggctta
     6481 gccgtatcat cgacagggtc gatttttccg ccttctgcca aaaacctaaa tggatcatcg
     6541 ggttcagcga cattaccgtc ctgcacgccc atcttttcac caactataag atcgcaacgc
     6601 tgcatgcccc gatggccggc gcgttcaatg acggctgcgc ggacacaccc ggtgtcctgt
     6661 cgctgaaacg cgtactcgcg ggcgagccga tgtcttatac ctgtgcccct catcccttca
     6721 acaggacggg ctatacccgc ggcatcctcg tcggcggcaa tctctccctg ctggcccatc
     6781 tcgtgggcag cgtttcggac atcgatacga aagggaagat cctctttctc gaggacgttg
     6841 gcgaatatct ctacaacatc gaccgcatgc ttcgtcagct taaacgaagt ggaaagctgg
     6901 atcatctcgc cggcctcgtc atcgggggat tcaccgaaac gaaagatacc atgcggccct
     6961 tcggcgccac ggtcgaagac atcatccgcg aggtcgtaaa ggaatacgcg taccctgtct
     7021 gttatggctt tcccgtcagt cacggcatag agaacgtcgc cctcaagatc gggacggaat
     7081 acacgcttac ggtcgcgccc ggttccaccg cccttacgga gtaaccacct catacgcatc
     7141 ctgtccgtcc tgtgatatcg gcagataata cgtgttgttg tacaccatca atgtatcccc
     7201 gttgatatcc tgctcgacgg cccccacgcg ccagcgcctt tatatttgcg ccagttaata
     7261 tcactgccgt caggaggaaa ggaagttttt taaacatggc ttttcatttt gaaggttctg
     7321 ataaagagac taattctatc aattaatgat tgttatttaa acggtaacag ccacccgatt
     7381 ttattgtttt tatgttgtac atttgagggt catccaataa gaacacgcta tgaaaaaatg
     7441 gctctcgttc cttctcctgc ccgctgtcct gtgggtcgcg tcctgcaaaa aaagcgggtc
     7501 gtcgaataag aatagcaata ataacgcctg gctttcctcg gtggtgagcc ttcagccgca
     7561 gacaagggta gtcgattcct tctattacga tagctcgcac cggatcaccg gctttcatca
     7621 gtacgagtac gattcgtctc agggatatcc cgcctttggg gaatggaccg tccagttcgc
     7681 ctatcccgcc ggtacggctt cacaaccttc gtcctatttg tatgtcgccg gattctcccc
     7741 ggatatccat gcgctgacct atgacgcaca gggacgcatc acccgcgata ccagcgtcag
     7801 cgggacgggt tatgtggcac attattccta tcccaacggc aatatcgcaa ccaccgtcct
     7861 cttcgacggg acaaacgcca acaaccagat cgatacgatg ttcctcagca acgggaatgt
     7921 ctccacactg cgcatctact atcccaatac ggcaggaacc gcagattcgc tcgagggcgc
     7981 cgtcaatttt ggattcactt cgctcgccaa cccggcctat catgccgaca taaccaatag
     8041 catcggcccg ctgctctata tcctgcagct ggacggcttc ggtagttcgg tcgaccctat
     8101 ctcccagcat gccctcaatt ccgtcggcgg ctccggctca ggtctgccgc ccggcgtaac
     8161 gctcaaatac aatcagacca ccgactcgca gggaaggctg atcgaacttt cgagcccgct
     8221 cggccccgtc gccggttcta tctcgttcac gtactactga tacgtatgac cttcgtccag
     8281 ctggaatata tcgtcgctgt ggacacttac cgccatttcg ccatcgcggc cggtcattgt
     8341 ttcgttacgc aacctaccct cagcatgcag gtccacaaac tggaggaaga actcggtctc
     8401 aagatcttcg accgcagcaa acaacccgtc atccccaccg aggccggacg cgagatcatc
     8461 gaacaggccc gccggatcct tgccgaaaaa gaagtcatcg gcgagatcgt acagacaaaa
     8521 aagggcatcc tcaccggcga gctgcgcata ggcatcatcc cgaccctcgc tccctatctg
     8581 ctaccgctgt tcgttcaatc gtttacggcg aaatatcccc aggtaaagct cgtagtgaac
     8641 gagttgatga cagacctcgt ggtctcgcgt ctgcgcgaag gtcgcatcga tgccggtatc
     8701 ctcgttacgc cgcttcagga gaacggcatc cgggaacagg tactctttta tgaggagctg
     8761 cttgtatatg tgtcccggaa aaataaggcc tataaaaaga cctacatgct ggcccgggat
     8821 atcgaccccg ggaaactatg gctgctggaa gaaggacatt gtttccgctc gcagatcgtc
     8881 cggctatgcg aactgaggaa agccagcaag gagggaagcc atttcgatta tgaagccggc
     8941 agcctcgaaa cccttcgccg gatggtcgag ctcaatgacg gcatcaccat ccttcccgaa
     9001 ctggccgtca gggatctcac cggcaaacaa caacaactga tccgccactt caggcgacct
     9061 gcgccgatgc gcgaggtcag cctggtaacc caccgcgatt ttgtaaaaca acgtctcgtc
     9121 caaaccctgt accaggagat cctccgggca gtccccgaaa aagtaaggca aaataaaaat
     9181 caacgcgtcg tccccatctg atcacccggc ttcagaaatg cctttagccg cgtatcgccg
     9241 ccgatcatgg taatgggatc cgtcacggca ggcagatatt gataggtagg aaaatgataa
     9301 tgcagcatca gtcccaccgg gtaaaggaac agccatttcc ggaaccggga tgtcatacta
     9361 taataaggat cgttcaggat cagtcgccgg caatttcgca ccgacgccaa ctgcgccgca
     9421 atacccgtcc caagccccgt cccgcagatc acgatctgcg aaggcttcca gcggctgcgc
     9481 gccagcttat agaatataag cgcataggca tacacattct tttcggtaaa tacgcccgta
     9541 ctcttgccaa aaccgggata atccatcacc catacctcat atccgcgcgg cgcaaaccca
     9601 agcgcgtcct gcgcattggt tgcaatgttt cctttgctat ccggaaaata caggacgaca
     9661 ccctccggca cgctgtccgc gggactggct ttgaattcga cgacatccag atttgtctgc
     9721 gcatcgtagg gcagatgtac ctcggtaaac ggacggtcga atgcaaagtt ctgcgtcgcc
     9781 ggcaacgtca caggatgcat taccaggcgg tcctgcgcat aatagatgcc gatcccgatc
     9841 agacaataga cgagcacgac caccttcaac caacgaaaga atagtttgcg cataggcgca
     9901 agctactgct tttgaggcac ctttaccggc gtctgcgagg ggagggtccc tgtatttttt
     9961 gccggtacgg tagtggcggt cttcgtcgcc tgggttgtac ctgtcatatt ttccgccgtc
    10021 agtttttgag cggtggctcc gaccgggccc gcaggcttga tgggtattga aatggttttc
    10081 ggggtcagcc gcggggtcgc gtcggactgt ttgatgggta cctggccggc cgtttgggac
    10141 attgccgtaa ctgcgaagaa gattaccggc aggaacaata caattatttt tttcatatta
    10201 tcattatttg acattgacca atacttttat ctttcccgca ggaccctcga aattttccag
    10261 cgccttgccg atggcctgac ccggctgcag acgatggagg tccgccttca tcgcatatcc
    10321 cgcccggctg ctcgtaacga gaatatcccc cctatggata ggcccgcctt cgtcgcaaac
    10381 cttggtgggg atgactccta cgacacccaa cgggaccttt ccggcaatgc taccgttcag
    10441 cgattcctcc gtcagcaaca cacccggctt cgtcgcataa acgcctgcga ccagcatgga
    10501 atacgctgcg gccgaacgca ccagcgtcct gtccttcacc tcggaaatga cgagcacatc
    10561 gccgggttcg tagtccccga atttaccttc gacgtcgaac acctcggcaa ggtctgcgcc
    10621 agaattctgt gtgccgccat cgaaatagcc cctgccggtg ttgtcgatcc tggctttatt
    10681 acctccatgc gattggaacc agaggatatc attgggtccc tcctggtcca ctacttccgc
    10741 aaccccctga tcattgctga ccacttgcag ggtgatgccg aaaccagttg tctcggtatg
    10801 ctcgaaaaat ccggcataac ctccagttcc cgaagagata ccataaatcc ctgccgtccc
    10861 ctcattccca aagatcgaat tgacctctcc tcttaccgca gcggcgactg caaagttatt
    10921 gttgacgaag aaattggagg cgttggcggt cgaatggtca ctgatgctgc cattgctgtt
    10981 ggtggtcacc gagaggacat tggatggatt gttggcattg tagttcacat aatatccggc
    11041 ggggttgatc ccgatggtct gcgcttgtaa cgtaggctgt gtattgttgg cattctgggt
    11101 ctggaaatcc gcgccgacgc ccagtccgta tgtgatggcc tccatcgcat agccgaaacc
    11161 ggccgtattg ctgtgttcga aataccctcc ataacctccc gtcccgctgg acgtaccata
    11221 gacaccggcc gtaccgttat tgccgaagat ggaattgacc tcgccgcgca cgccggcccc
    11281 gaccccgaaa ttgttattgt tgaaaaagtt gccggcattg ccgaaggaat gatcggggat
    11341 atcgccctgg gtgttcgttg tcacattcag ggtattggcg atattgttgg tattggagtt
    11401 aa
//
LOCUS       DASYSV010000061        16800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2062473, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000061 DASYSV010000000
VERSION     DASYSV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 16800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16800
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062473"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="VGQ51_01815"
     CDS             <1..1758
                     /locus_tag="VGQ51_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035320.1"
                     /translation="YGKNDYNDYGKAYYPSYNTYTYTQVGSHKNNGSGTNSPTGTQNV
                     LYNYNSFANKTITNGNQLQNSYSGTKNWQATEGLSYDNRFGDHEISATLVSEQEQTTG
                     NVYSTTDNTQVIPGVDQLYGFSSDGSNLSISGQTVSTGRWSYLGRLNYSYKGKYLLEG
                     SFRDDASPNFPIEHQWGFFPSASVGWKISEENFFQNHVHFLDDLKIRFNLGLTGNDAG
                     QPFGWVERYTSANGYLFGGTNTNGLSTTQVPNPEITWESALFKDLGVDGTFANRKFNF
                     TFDYWFKHGFNMLEPPQTTVTNSSGITSTASVNHGIINSWGTEFQLSYHQMVNKDWTV
                     FATVNFNWSDNKVIKEYYNPGTDTGYLYPIGKRIDQGITGMKSTGIVRTAAEASDWNS
                     KHPTWTVNKDTLRAGDLNFVDVNGDGKIDGNDQTRIADRSSTLFGLGFNFGAQWKGLR
                     ISTNISLGIGGQIAWKKVDIAPPTKDAASLSMWKGSYTAYRTNAALPAIYAPFANQVS
                     TFWLHSATYMYVNNMMLSWQVPVSFTSTHHLPETRFYVTGTNLWSIIDPTPYKDPRSN
                     EITDYPILRNWTFGANISL"
     gene            1789..3633
                     /locus_tag="VGQ51_01820"
     CDS             1789..3633
                     /locus_tag="VGQ51_01820"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9035321.1"
                     /translation="MKKLITYSLLLIGLANAGCQKQILDKVDFNGVPVTTAWNNAATA
                     NVYLDDLYQLCMPVYYSTASISTIPTNWHNISDECNGGSNIGVLQGTLTQESETDFFP
                     TKNSSYQWIRKINILLTDIDKYGLTPDVTAPIKAQAYFLRAWTYFQLVKVYGGVPYIT
                     KVLDYVNDSLNIPRNTTTQCVDSMISDLNHCSILPAHWAGADMGRITSGAALALKGRL
                     LLYYASPQFNPTGDQARWQLALQANKAAYDSLTAEGYALYPKYSRIFLDASLATNTEP
                     IIFRAYNGSSVVDQDANNWDNTVRPYSQAYSGGGKNYNPTWNLVSAYPMADGYAAGSP
                     QSKYAYDSIRFWHNRDPRFYSTVVYNGAVYGLGNTAGRIQYTYVGTGEDLSAPTTTGF
                     YCRRNVDTSLASSATQYGKTWWVEMRFAEVMLNYAECANATGDQATAYTMLEAIRKRA
                     GISANGDGFYGLTQGMTQTQMATAILNERQIEFAFEDKRYDDLRRTRSFDALNGQIRM
                     GFFIVPRAPYFASTKSSDANKANNIERYISGTTGPKIRDTINVEGPAYQVFFKDSLAP
                     IANEKPINFQTTYYSYGIPSTNIQKQPALQQTAGWTYLGAAGTFDPTR"
     gene            3660..5300
                     /locus_tag="VGQ51_01825"
     CDS             3660..5300
                     /locus_tag="VGQ51_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase"
                     /protein_id="HEV9035322.1"
                     /translation="MKKRFFLLVMVAASLGLSRKATAQYPNVPEDVKAQSEAMLKAAW
                     AHSDSAWQVAWPIIDDEARHGKPYVPWASRPTDLPQSALLAFPGAEGGGAHSFGGHGG
                     RVLVVTNLDDSGPGSLRWACEQGGARIIVFNVAGIIHLHSPIIVRAPYVTIEGQSAPG
                     DGICVAGESFWVNTHDVVIRYMRFRRGETNVGRRDDALGGNPVGNIIVDHTSTSWGLD
                     ENFSMYRHMFDPGDGSKEEKLGTVNITIQNCISAESLDTWNHAFGTTMGGENCMLVRN
                     LWADNTGRNPSIGWNGVFNFVNNVVFNWHHRSMDGGDYTSLFNIINNYFKPGPVTPKD
                     ENVGHRILKPETGRSKLKELHFGRAYVNGNIMDGYPAVTKDNWDGGVQIGEEKDAGAY
                     KPYMKWDKPFPMPPVKILPAEEAYRFVLANVGATLPHRDAVDSRVIEQVRTGKIAYKE
                     VNTDTLPQFHVRKLPKDSYKEGIVTEVFEVGGYPDYKGTAYKDSDHDGLPDEYEIKHG
                     LDPHDPNDSAKPAKDGSGYSNIEVWLNNLASRGEKAVK"
     gene            5305..8373
                     /locus_tag="VGQ51_01830"
     CDS             5305..8373
                     /locus_tag="VGQ51_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HEV9035323.1"
                     /translation="MRSFIIGCFLVSVSLPLFTYAQKKTKEVPPPPVAVRGGHLVYTP
                     DGRGDRIPDFSYSGYRGGDVPIPDVPVHITVPLHVAAPGAVKDDDATSRIQAALDFVS
                     GLPAGADGIRGAVLLDKGVYHISGSLLIRHSGVVLRGSGMGGDGTILRATGHDRRTLI
                     RIAGVDDRTTSTEVTIADAYVPVGAMHLHVTGSQPFHKGDLVLIHRPSTAAWIRLLGM
                     ETFGGGISALGWKPGQRDIFWDRRITAVDGSDITLDAPITTALDTAYGGGRIAGYRWP
                     GRIDEVGVENLRCVSDFDIHNPKDEAHSWMAITLENVSDAWVRQVVAEHFAGSAVMVL
                     GTANRITVEDCKYLEPVSEIGGQRRNAFLTMGGQTLFQRLYSERAMHDFGVGFCAPGP
                     NAFVQCQSHYSYGISGAIDSWASGILFDVVNVDGNTLGYFNREQDAQGAGWCGANSLL
                     WNSTASRIDCYRPPGAENWSYGSWGQFGGDGYWNESNSSVQPRSFFYAQLGDRLGADA
                     ARRGQVLPIENEASSSPTVEQAAALTAWSVKPRVQMTEWIDQAAERNPIPTAPGNAPS
                     VDKIGVRQPLTNAKVAPMHVSDGWLVRGNAVLTGHRYLEPWWEGSVRPYGVAEARPAI
                     TRWVPGRTGTGLTDDLDAMTDTMLERHVVALDHNYGLWYDRRRDDHERIRRADGDVWP
                     PFYELPFARTGKDTAWDGLSKYDLTKYNPWYWDRLRRFADLADEKGLVLLYQDYFQHN
                     IIEAGAHNADFPWRPANNINHTGFPEPTPYAGDKRVFLAEQFYDTANPARHDLHRAFI
                     RQCLDNFADNTGVIQLTSAEFTGPLHFMQFWIDNIRQWEQEKSRREIIGLSATKDVQD
                     AILADPVRAAVIDVIDIRYWYYQQDGKAYAPLGGQSLAPRQHERLLKPKRSSFEQVYR
                     AVREYRERYPTKAVIYSAEGENFGWAVFMGGGSLANIPPVSEAGFLDAASQMAPVDIP
                     GKPQGQYALAGKDGYIIYSAKGELAVGTKGYRVHRISADGSAFVYWLEKK"
     gene            8377..9723
                     /locus_tag="VGQ51_01835"
     CDS             8377..9723
                     /locus_tag="VGQ51_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HEV9035324.1"
                     /translation="MRIFWMILLGCLTVHRPAAELPLLHVSPDGHYLMTDKGAPFFWL
                     GDTGWLLLSRLTTGQAEQYFTNRHTKGFNVIQVMVLHGLGDVDVYGDSALVRRRLAMP
                     TGKGYWEHLDSLVDMAAAKDIYLALVPVWGGVVKSAKATPDQARAYAQFLALRYKNKT
                     NIIWMNGGDIKGSDYEAVWNTIGTTLRENDPHHLITFHPRGRASSSFWFEGQSWLDFN
                     SVQSGHRSYAQDTSAGDPHYGEDNWKYIRADYTKHPVRPVLDAEPSYEQIPHGLHDTS
                     QPRWTAADVRRYAYWSVFAGGCGFTYGDNSVMQFLRPTDKGSAYGARAPWFRAIDDPG
                     AGQMNYLKKLMLSRGGYFTRVPDTSMITDQGTRYDYVTATRGKDYAFIYTYTGRTFTV
                     NPTALGSRRLQASWYDPKDGTTKTIGVFSGRETLTFDPPGNPAPGNDWILILDDHE"
     gene            9716..10666
                     /locus_tag="VGQ51_01840"
     CDS             9716..10666
                     /locus_tag="VGQ51_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEV9035325.1"
                     /translation="MNKVYRILLPLLLLASCKPAKDIYLFTSFREPAVDGLHFLYSRD
                     GKHWNDIPGSWLTPEVGGDSSTKPTAGGKKIMRDPSMAQGPDRVWHLVWTCGWNGDRG
                     FGYASSRDLIHWSPQQFIPVMQHEPDAFNVWAPEIFYEKDSAQYIIVWATTIPGRFPK
                     GMEDEKNNHRLYCTTTRDFVTFTPTRLYYDPGYSVIDAMILQRGPKDFVLVFKDNTRL
                     QRNIKVAFSDSPAGPWHDDSKAFTPEFTEGPTVAKVGAEYYIYFDGYRAHKYGAVKTS
                     DFRTFTDASDEVSVPAGHKHGTIVMTDKKTLKRLIHEREK"
     gene            10650..11453
                     /locus_tag="VGQ51_01845"
     CDS             10650..11453
                     /locus_tag="VGQ51_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEV9035326.1"
                     /translation="MSEKNSRRNFLKTAALGGMMATAIPEIVSAAMAKDGPSAKTPHA
                     PSEPSKAKKIRLNDGDVVLFQGDSITDWHRKRDDDKPNSGGALGTGYPFFVTGELLCR
                     WPGRDLKCYNRGVSGNKVYQLADRWQTDCLDLKPTVLSILVGVNDFWHTLVNGYKGTI
                     KTYRDDYKTLLDRTKQALPDVRLIIGEPYAVIGVKAVDDKWYPAFNEYRAAAKEIAES
                     FGAVFIPYQEIYDKAQRSAPGSYWTIDGVHPSLAGARLMAEAWVATIHG"
     gene            11455..13266
                     /locus_tag="VGQ51_01850"
     CDS             11455..13266
                     /locus_tag="VGQ51_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-hairpin glycosidase"
                     /protein_id="HEV9035327.1"
                     /translation="MKFAIHTIFLLSLTATVSAQDSELMHYSGKVLSNIDYHHGMLVP
                     AVGVHSIQVLRANREHPDSAEGSGYTYNHAPMLAYWNNKFYLEYLSDRVGESVPPGQT
                     LVTTSENGYTWAKPVVVFPPYKIPDGTTKEGHPGVAKDLYAVMHQRMGFYVSKDHRLL
                     VLGFYGICLDPHDDPNDGKGIGRVVREVHADGSFGPIYFIHYNPAWTTGNTSYPLYTS
                     SKDKDFVRDCNELMATPLMMMQWVEETDRKDPLIPLHKDYKAFCFYHLPDGHVVGMWK
                     NGLTGTSKDEGRTWSRVVRAPHVVTSNAKMWGQHTSDGKYATVYNPSEFRWPLAISTS
                     KDGLDYTDLLLVNGEISTARYGGNYKNYGPQYTRGIIEGNGTPPDPNIWITYSMNKED
                     IWVSTIPVPVTAAVAADVNDDFGKADLKQWNTYSPRWAPVGLVDHTLQLRDADPYDFA
                     MAERVFPVAKKMQVEFSLAAGQNDHGQLQIEMQDEHNLPAIRLSFEPDGTLIAKAGAR
                     IKKILSYLPGVVYTIRISVDADTRMYTVNIDGKDVLTQLFFQPVESFGHIVFRTGETR
                     HFPTADSPAEADTDRPKAGDPVPMASYSIRYLKTTRE"
     gene            13263..16508
                     /locus_tag="VGQ51_01855"
     CDS             13263..16508
                     /locus_tag="VGQ51_01855"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEV9035328.1"
                     /translation="MSGRVFIGMALLLAASLKAGAQRNVERPVQPEDVTAIGGFAGSR
                     IEKNQRNYIKTFDIAKYVGLMETRDFTGWDWRQGEQPGKWLEAAIWASTRTHDTALSN
                     EAHIMLDRIIRSQAPDGYIGITSQTVRTPERPLGGMDAYELYFLEHALLTANDLWHDR
                     AALRAARRLGDYFVRYIGPGKAEFWPSPLRPPENKDKVLKGTMHSEIAGHSIHYGWEG
                     TLFIDPMMRLYQETGDRRYYDWCKWVVNSIDKWSGWDSYSNLDKVAADSMPINKIQPY
                     VHAHTFQMNFLGLLRMYAVSGDTSYLRKVEGAWKDIASRQLYITGGVSVGEHYERDYI
                     KPLTGEMMETCATMSWMQLSQALLDITGDPKYADAMGRILWNNVFAEQTIDGDANHYF
                     TPPNGVTPRGYYREPDCCMGSGERLLAMLPGFIYAKGAGRSIYINQFIASSTVIDGTR
                     ITQTTDFPESGKVTVSVGPAKAGVFAVYVRIPGWADSAVVAVNGVAVQRVRPGSYLKL
                     ERTWRSGDKIGVELPMQLHWVRHEHYEKTADRKPYKTAPDNDAPYALLRGPVVYALDD
                     IWYKGDTAVFRPMDSIRYVLSDPDGMRRIGAPGPDILGPGYEVRLILADGSETSIPVY
                     PFANIGKWYRDAAHKPDSNAAVWKYAVWLKGSDAASGRLSLPSIFGDNMVLQRNVPVP
                     VWGDADAGSTVTVHFGSQTRSVVADGKGHWRVSLGPLSASTEGVEMTVTGDGASIRFR
                     DVLVGEVWLCSGQSNMEYSMRKNSKFEKGGDALDSARNPLIRIFLVRNNYSKPDRHIR
                     RRWDSAEGAPLRDFSAAGYFFAQEVFRRLNVPVGVISNAIPGSLIEPWLPDSGKFYKT
                     LVTPLAPYALKGFLWYQGESNCFLADTLRYAGKMKTLIESWRTLWANPDLSFYFVQIA
                     PYYYSKTTGSTGTEPVFWEAQEKALSLPHTGMVMTTDLVDTPFDLHPAYKWKIGRRLA
                     LLALANDYHEAVVCSGPVFDHLTVRGRRVELHFTGGPLVSRKPLRWFETEDKSGKFTP
                     ATAAIAGDDKIILKARNRPIAVRFGWNEAAQPNLFNKEGLPAMPFRATTNE"
     gene            16501..>16800
                     /locus_tag="VGQ51_01860"
     CDS             16501..>16800
                     /locus_tag="VGQ51_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEV9035329.1"
                     /translation="MNKLLSISLLALLPAFGQGQETQRVFLSGTGNDHTVDWQFYCTE
                     GRNSGKWTTIPVPSNWELQGFGKYNYGLDKDSVRGHEKGLYKYRFTIPADWKGREV"
BASE COUNT         3901 a         4591 c         4769 g         3539 t
ORIGIN      
        1 tacggcaaaa atgactacaa cgattatggc aaggcctatt atcccagcta taacacgtac
       61 acctataccc aggtaggcag ccataagaac aatggctccg gcacaaattc cccgacggga
      121 acccagaatg tcctgtacaa ctacaattcg ttcgccaaca agacgatcac caacggcaac
      181 cagcttcaga acagctactc gggcaccaag aactggcagg ccaccgaagg actatcatac
      241 gacaaccggt ttggcgatca tgagatctcg gcaaccctgg tatccgagca ggagcaaacg
      301 acaggaaatg tctatagcac taccgacaat acccaggtca tcccgggcgt cgatcagctc
      361 tatggtttca gctcggacgg cagcaatctc agcatcagcg gtcagacggt atctacagga
      421 cgctggagct atctgggacg actgaactat tcttataaag gtaaatatct tctcgaaggc
      481 tcattccgcg acgatgcctc gcccaatttt ccaatcgaac accaatgggg cttcttccct
      541 tccgcctccg taggttggaa aatatccgag gagaatttct ttcagaacca cgtccacttc
      601 ctggacgatc tgaagatcag gttcaacctg ggtctgacgg ggaatgatgc cggtcagcct
      661 ttcggatggg tggaaagata tacctctgct aacggatacc ttttcggagg cacgaatacc
      721 aacggacttt cgacgacaca ggtgccgaat ccggagatca cctgggaaag cgccctgttc
      781 aaagacctcg gcgtcgacgg aacctttgcg aaccgcaaat tcaactttac tttcgattac
      841 tggttcaagc atggcttcaa tatgctcgag ccgcctcaga cgacggttac caattcttcg
      901 ggtattacca gcaccgcatc ggtcaaccac ggtatcatca atagctgggg tactgaattc
      961 cagctcagct atcaccagat ggtcaacaag gattggactg ttttcgcaac cgtcaacttc
     1021 aactggagcg acaataaggt gatcaaggaa tattacaatc cgggcaccga cacgggttat
     1081 ctctatccca tcggcaaacg catcgatcag ggcatcaccg ggatgaagtc cacggggatc
     1141 gtccgtacgg cggccgaagc gtcagactgg aattcgaaac accccacctg gacggtcaat
     1201 aaggacaccc ttcgcgcggg cgatctcaat ttcgtcgacg tgaatggcga cgggaagatc
     1261 gatggcaatg atcaaacccg gatcgctgac cgctccagca cgcttttcgg cctcggcttt
     1321 aattttgggg cacagtggaa aggattaagg ataagtacga atatctcgct gggtatcgga
     1381 ggtcagatag catggaagaa agtggatatc gccccgccga ccaaggatgc tgcatcgctg
     1441 tccatgtgga agggatccta tacggcatat cgtaccaatg cggccctgcc cgcgatctac
     1501 gcgccgttcg ccaaccaggt gtccaccttc tggctgcata gcgccaccta tatgtatgtc
     1561 aacaacatga tgttgtcctg gcaggtaccg gtatccttca cctccactca ccatttgccg
     1621 gagacgaggt tttatgtcac gggcaccaac ctctggtcca tcatcgaccc tacgccttac
     1681 aaggatcccc ggtccaacga gatcacggac tacccgatcc tgcgtaactg gacctttgga
     1741 gccaacatca gtctataacc attgctgctt tataaaaaat atcaaaagat gaaaaagctt
     1801 atcacatatt cccttttgct gatcggtctg gcgaatgccg ggtgtcagaa acaaatactc
     1861 gacaaggtgg acttcaacgg agtgcccgtc acgacggcct ggaataatgc ggcgacggca
     1921 aacgtgtatc tcgacgatct ctaccagctg tgtatgccgg tatattacag cactgccagc
     1981 ataagcacca tcccgaccaa ctggcataat ataagtgacg aatgcaacgg cggttccaat
     2041 atcggcgtac tgcagggtac gcttacgcag gagagtgaga ccgacttttt ccctaccaag
     2101 aactcctcct atcagtggat ccgcaagatc aatatcctgc tgaccgatat cgacaaatac
     2161 gggctgacgc cggacgtcac ggctcccatc aaggcccagg cctatttcct gcgcgcctgg
     2221 acgtatttcc agctggtaaa agtgtatgga ggcgttccat atatcaccaa ggtgttggat
     2281 tatgtaaacg acagcctgaa cataccacgg aacacgacca cgcaatgtgt agacagtatg
     2341 atctcggatc tcaaccattg ctcgatcctt ccggcgcatt gggccggagc tgatatgggt
     2401 cgcattacca gcggcgcagc gcttgccctg aagggaaggc tactgctata ctatgccagc
     2461 ccgcagttca accccaccgg cgaccaggcg cgctggcagc tggcattgca ggcaaacaag
     2521 gcggcctatg attcgttgac ggcggaagga tatgcgttgt atcccaaata ttcccgtatc
     2581 tttctcgatg ccagtctggc gaccaacacc gagcctatca ttttccgggc atacaatgga
     2641 agcagcgttg tagaccagga tgccaacaac tgggacaaca cggtccgccc atattctcag
     2701 gcttacagcg gcggaggtaa gaactacaac ccgacctgga acctcgtatc cgcatatccc
     2761 atggcggacg gctatgctgc gggaagcccc cagtccaaat atgcctatga cagcatccgg
     2821 ttctggcata accgcgaccc gcgtttttat tccactgtcg tatataatgg cgccgtctat
     2881 ggtctcggga atacggccgg ccggatccag tatacatatg taggtacggg tgaagacctg
     2941 agcgctccga cgaccaccgg gttctattgc cggaggaatg tggataccag ccttgccagt
     3001 tcggctaccc agtacgggaa gacatggtgg gttgaaatgc gctttgccga ggtcatgctc
     3061 aactatgccg aatgcgccaa tgcaaccggc gaccaggcta cggcatatac catgctggag
     3121 gccatccgca aacgtgcggg tatctcggcc aacggagacg gcttttatgg attgacacag
     3181 ggcatgaccc agacgcaaat ggcgacggcc atcctgaatg agcggcagat cgaattcgca
     3241 ttcgaagaca agcgttacga cgacctgcgt cgtacgcgct ccttcgacgc gctgaacggc
     3301 cagatacgga tgggcttctt tattgtgccg agggcgccgt atttcgcctc caccaaatcg
     3361 tccgatgcca acaaagcgaa caatatcgaa cgttatattt caggaaccac cggccccaag
     3421 atcagggaca ccataaatgt cgaagggcca gcctaccagg tattcttcaa ggacagcctg
     3481 gcgccgatcg cgaacgaaaa gcccatcaat ttccagacta cctattattc ttacggtatc
     3541 ccctccacga acatccagaa acagcctgct cttcaacaga cggccggatg gacctatttg
     3601 ggagccgcgg gtacgttcga tccgaccagg tgatccatcg catctaaaca caataaacaa
     3661 tgaagaaacg attttttcta ttggtaatgg ttgccgcttc gctgggtctg tcccgcaagg
     3721 cgaccgccca atatcccaac gtacccgaag atgtcaaggc ccaatccgaa gccatgctga
     3781 aggctgcatg ggcgcatagt gattccgcgt ggcaggtggc ctggcccatc atcgacgacg
     3841 aggccaggca tggcaaaccc tatgttccct gggcatcgag gcctacagac ctcccgcaat
     3901 ccgcgttgct cgcctttccc ggcgccgaag gcggcggggc acacagcttt ggcggacacg
     3961 gcggacgcgt cctcgtggtg acgaatctgg acgattctgg tcccggttcg cttcgctggg
     4021 cctgcgagca gggtggggcg cgcatcatcg ttttcaatgt agcgggtatc atccatctgc
     4081 atagcccaat catcgtccgt gcaccttatg tcaccatcga aggtcagtcc gccccgggcg
     4141 acggtatctg tgtggccggg gaatccttct gggtcaacac tcatgacgtg gtcatacgct
     4201 atatgcggtt tcgccgcggc gagacgaatg tcggacgtcg tgacgacgca ctcggtggca
     4261 acccggtagg gaatatcatc gtcgaccata cttccaccag ttggggactg gatgagaact
     4321 tttcgatgta tcgccatatg ttcgatccgg gtgacgggtc gaaagaagag aaattaggca
     4381 ccgtcaatat tactatccag aattgtatct cggctgagtc actcgatacc tggaaccatg
     4441 ccttcggaac gacgatgggc ggcgagaact gcatgctcgt ccgcaacctc tgggccgata
     4501 atacgggacg caatccctcc atcggctgga acggcgtttt caatttcgtc aacaacgtcg
     4561 ttttcaactg gcatcaccgg tcgatggacg gtggcgatta taccagcctg ttcaatatta
     4621 tcaacaatta tttcaaaccc ggtcctgtaa ctccgaaaga cgagaatgtc ggccatcgta
     4681 tcctgaaacc cgagaccggg cgcagcaagc tgaaggaatt gcatttcgga cgcgcctatg
     4741 tcaatgggaa catcatggac ggctatcccg cggtcacgaa ggataactgg gacggaggtg
     4801 tgcagatcgg cgaggaaaag gacgccggtg cttataaacc ctatatgaaa tgggacaaac
     4861 ccttccccat gccgcccgta aagatccttc ccgccgagga agcctatcgg ttcgtccttg
     4921 ccaatgtcgg cgccacgctg ccgcatcgcg acgccgtaga cagccgtgtc atcgagcagg
     4981 tgcgtaccgg taaaatagcc tataaagaag tgaatacgga tacccttcca cagtttcatg
     5041 ttcgtaaatt gccgaaggat tcctataaag aagggatcgt taccgaggta ttcgaggtgg
     5101 gtggctatcc cgactataag ggtacggcct acaaggattc cgatcatgac ggtctgccgg
     5161 acgaatatga gatcaaacac ggtctcgatc cgcacgatcc gaatgactcc gccaagccgg
     5221 cgaaagatgg cagcgggtat tcgaatatag aagtatggct caataacctc gcgtcgagag
     5281 gcgaaaaggc ggtaaaatag aaatatgcgg tcgtttatca taggttgttt tttggtatcg
     5341 gtctcgttac ccttgtttac gtatgcacaa aagaagacca aagaggtgcc gcctccgccc
     5401 gtggctgtgc ggggcggcca cctggtctac acccccgatg gtcggggtga ccgtatccct
     5461 gatttttctt acagcggata cagaggcggc gatgtgccga tccccgatgt acccgtccac
     5521 atcaccgtcc cgctccacgt cgccgccccc ggcgccgtca aggacgatga cgccacttca
     5581 cgaatacagg ctgccctcga ctttgtatcg gggcttccgg ccggcgccga cggtatccgc
     5641 ggcgccgttt tgctggataa aggcgtgtac catatttccg gaagcctgtt gatccgccat
     5701 tccggcgtcg tccttcgcgg tagcgggatg ggcggggacg gtacgatcct ccgggctacg
     5761 ggtcatgacc gccggaccct gatccggatc gccggtgtgg atgaccggac aacgagtact
     5821 gaagtcacta tcgccgatgc ctatgtgccg gttggcgcca tgcatcttca tgtgacgggg
     5881 tcgcaacctt ttcacaaagg ggacctggtt ctcatccatc ggccttcgac ggcagcctgg
     5941 atcaggctgc tggggatgga gaccttcggc ggcgggatct ccgcgctggg atggaaacct
     6001 ggccaacggg atattttctg ggaccggcgg atcacggccg tcgacggcag cgatattacc
     6061 ctcgatgcgc ccatcacaac ggcgctggat acggcctatg gcggcggtag aatagcaggt
     6121 tatcgctggc cgggccgtat cgacgaggta ggagtggaga acctgcgatg cgtctccgac
     6181 tttgacattc ataacccgaa ggacgaagcg catagctgga tggccatcac actcgaaaat
     6241 gtatccgacg catgggttcg ccaggtggta gccgaacatt tcgcgggttc ggcggtaatg
     6301 gtgcttggga ccgccaatcg tatcacggtg gaagattgca aatacctgga gccagtctca
     6361 gagataggag gacaacgccg caatgcgttt ctgacgatgg gcggtcaaac cttgtttcag
     6421 cggctgtatt ccgaacgcgc catgcacgat ttcggcgtag gtttctgcgc acccggcccc
     6481 aatgcctttg ttcaatgcca gtcgcactat tcctatggca tcagcggcgc catcgacagc
     6541 tgggcctcgg gtatactttt cgacgtggtc aacgtcgacg ggaatacgct ggggtatttc
     6601 aaccgcgaac aggacgccca gggagcagga tggtgcggag ccaacagtct gctctggaac
     6661 tctacggcct cgaggatcga ttgttaccgc ccgccgggcg ccgaaaattg gtcctatgga
     6721 tcatgggggc agtttggcgg cgatggttac tggaacgaat cgaattcctc cgtacaaccc
     6781 cgcagctttt tctatgctca gctgggggat cgtctcgggg cggatgcggc gcgtcggggg
     6841 caggtgttgc ccatagagaa tgaggcatcg agcagtccta ccgtcgaaca ggctgccgcg
     6901 ctgacagcgt ggtcggtcaa accgcgggta cagatgaccg aatggatcga tcaggctgca
     6961 gaacgcaatc cgatacctac ggcgccgggt aatgctccat cggtggacaa gatcggtgtg
     7021 cggcagcctc tgacaaatgc gaaagtcgcg ccgatgcatg tttccgacgg ttggcttgtc
     7081 cgcggtaatg ccgtactcac gggacatcgc tatttggagc cgtggtggga gggatcggta
     7141 agaccctatg gggtagccga agcccggcca gccataacgc gctgggtccc cggccgtacc
     7201 ggtaccgggc tgaccgacga cctggatgcg atgaccgata ccatgcttga aagacatgtc
     7261 gttgctctgg atcataacta tggtctatgg tatgaccggc gtcgcgatga tcatgaacgc
     7321 atacgtcgtg ccgacggcga tgtctggccc ccattttatg agctgccctt cgcgcgcacc
     7381 ggtaaggata cggcctggga cggtctcagc aaatacgatc tgaccaaata caatccatgg
     7441 tactgggatc gcctccggcg ttttgccgat ctggccgatg agaaaggatt ggtcctgttg
     7501 tatcaggact attttcagca taacatcatc gaagctggcg cgcacaatgc cgatttccca
     7561 tggcggcccg ccaacaacat caatcatacc ggcttccccg agccgactcc atacgccggc
     7621 gacaaacgcg ttttcctggc agagcaattc tatgatacgg ccaatccggc ccggcatgat
     7681 cttcaccgcg cttttatccg gcaatgtctc gataactttg ccgataatac gggggttatc
     7741 cagctgacga gcgccgaatt caccggaccg ctgcatttca tgcagttctg gatcgacaat
     7801 atccgtcagt gggagcagga gaagagccgc cgcgagatca tcggtctcag tgcgaccaaa
     7861 gatgtccagg atgcgatact ggccgacccg gtgagggccg cagtcatcga tgtcatcgac
     7921 atccgctatt ggtattatca acaggatgga aaagcatatg ccccgctggg cggacagagt
     7981 ctggcgccgc ggcagcacga gcggttgctc aaaccgaagc gttcgtcctt cgaacaggtc
     8041 tatcgcgcgg tcagggaata ccgggagcgg tatccgacaa aagcggtgat atattcggcg
     8101 gaaggcgaga actttggctg ggccgtattt atgggcggcg gttcgctggc gaatatcccg
     8161 ccggtgtcag aggccggttt cctcgacgca gcatcacaga tggcgccagt ggatattccg
     8221 ggcaagccgc aggggcagta cgctctggcc ggaaaagacg gatacatcat ctattcggcc
     8281 aaaggagaac tcgccgtggg cacgaaaggc tatcgcgtgc accggatatc cgcggacggt
     8341 agcgcgttcg tatattggct ggagaaaaaa tagtctatga gaattttctg gatgatatta
     8401 ctgggttgtt tgaccgtgca tcgccccgcg gcagaactgc cattgctgca tgtctcgccc
     8461 gacgggcatt acctcatgac ggataagggc gcaccttttt tctggctggg ggacaccgga
     8521 tggctgctgc tgagccgtct gaccacaggg caggcggagc aatatttcac caaccgccac
     8581 acgaaaggct tcaacgtgat ccaggtgatg gtgctgcatg gtctcggaga cgtcgatgtc
     8641 tatggggaca gtgcgctggt gcgtcggcga ttggcgatgc cgacgggcaa aggctattgg
     8701 gaacatctcg actctctcgt cgatatggcc gctgcaaaag acatctatct cgcgctggtg
     8761 cccgtctggg gaggcgtggt caaatcggca aaagcgactc cggaccaggc acgggcatac
     8821 gcgcaatttc tggctctccg ctataagaac aagaccaaca tcatctggat gaacggcggg
     8881 gatatcaaag gatccgatta tgaagccgtc tggaacacga tcggaacgac gctccgcgaa
     8941 aatgacccgc atcacctcat cactttccat ccgcgcggac gagcctcttc ttccttctgg
     9001 ttcgaaggac agtcatggct ggatttcaac agcgttcagt ccggtcaccg cagctatgcc
     9061 caggatacct ccgccggcga cccgcattac ggcgaggaca attggaaata cattcgtgcc
     9121 gactacacca aacacccggt gcgacccgtg ctcgacgcgg aaccgtccta cgagcagata
     9181 ccccatggac tgcacgatac cagccagccc cgctggacgg cggctgatgt tcgccgttat
     9241 gcctactggt cggtgtttgc aggaggctgc gggttcactt atggcgacaa ttccgtcatg
     9301 caatttctcc gccccacgga taaaggatcc gcatatgggg cacgtgcgcc gtggtttcgc
     9361 gcgatcgacg atcccggcgc cggccagatg aactatttga agaaactcat gctctcgcgg
     9421 ggtggctatt tcacccgcgt gccggatacg tcaatgatca ccgaccaggg tacccggtac
     9481 gactatgtca ctgctacacg cggaaaggat tacgcattta tatacaccta tacagggcgt
     9541 accttcaccg tcaaccccac cgcactcgga tcgaggagac tacaggcatc ctggtacgac
     9601 cctaaagacg gcactaccaa aactatcggc gtattttcag gccgggagac gctgaccttc
     9661 gatccgcccg gtaaccctgc acccgggaat gattggatat tgatccttga cgaccatgaa
     9721 taaagtatat cgcatcttgc ttcccttgct gctgctggct tcctgcaagc cggcaaagga
     9781 catctatttg ttcacctctt tccgcgagcc ggccgtcgac ggcctgcact ttctgtacag
     9841 ccgcgacggt aagcattgga acgatatccc gggctcctgg ctgacgccgg aggtcggcgg
     9901 cgactcctcg accaaaccga cggccggagg gaaaaagatc atgcgtgacc cttcgatggc
     9961 gcagggcccc gacagggtat ggcatctcgt atggacctgc gggtggaacg gcgaccgtgg
    10021 attcggttat gcttcctcac gcgaccttat ccattggagc ccgcagcaat tcattccggt
    10081 catgcaacac gaacccgacg ccttcaatgt ctgggcgccc gagatctttt atgaaaaaga
    10141 cagcgcgcag tacatcatcg tctgggcgac gacgattccg ggtcgttttc ccaaagggat
    10201 ggaagatgaa aagaacaatc accgtctata ctgcacgacc acccgcgatt tcgtgacctt
    10261 tactcctaca cggctttatt acgatcccgg gtatagcgtc atcgacgcga tgatcctgca
    10321 acggggacct aaagattttg tcctcgtatt caaggacaat acgcgtctgc aacggaatat
    10381 aaaagtggcc ttcagcgaca gtcccgccgg cccatggcac gacgattcga aggcatttac
    10441 tcctgaattt accgagggac ccacggtcgc aaaagtgggc gcggagtatt acatctattt
    10501 cgacggttat cgtgcgcata aatacggagc ggtgaagacc agcgatttca ggacctttac
    10561 tgatgcctcg gatgaggtgt cggtgccggc ggggcataag cacggaacca ttgtgatgac
    10621 cgataaaaaa acacttaaac gattgataca tgagcgagaa aaatagtcgt cggaattttt
    10681 tgaaaaccgc cgccctgggc gggatgatgg cgaccgcaat cccggagatc gtgtccgccg
    10741 caatggctaa agatgggcct tcggccaaga cgccacatgc gccttcggaa ccttcaaaag
    10801 caaagaagat caggctcaac gacggggacg tcgttctctt ccagggcgac agcattaccg
    10861 attggcatcg caaacgcgat gatgacaaac ccaacagcgg cggcgcactc ggtacaggtt
    10921 atcctttttt cgtcacgggt gaattgttat gccggtggcc cgggagagat ctgaaatgct
    10981 ataacagggg cgtcagcggc aacaaggtct atcagctggc cgatcgatgg cagaccgact
    11041 gtctcgatct gaaaccgacg gtcctcagca tccttgtcgg cgtcaacgat ttctggcata
    11101 cactggtcaa tggatataag ggcacgatca aaacttaccg ggacgattat aagacattgc
    11161 tcgaccggac caaacaggct ttacccgacg tgcgtctgat catcggagaa ccctatgcgg
    11221 tcatcggtgt aaaggcggtg gatgataaat ggtatcccgc ttttaatgag taccgggcag
    11281 cggcaaagga gatcgccgaa agcttcggcg ccgtctttat cccttaccag gagatctatg
    11341 acaaggcgca aagatcggct ccggggtctt attggacgat cgatggcgtg catccaagcc
    11401 tggccggtgc ccgtctgatg gcggaggcct gggtggcgac catacacgga taacatgaaa
    11461 tttgcaatac atacgatctt tctcctttcg ctgaccgcga ccgtaagtgc gcaggacagc
    11521 gagctcatgc actacagcgg gaaggtgctg tcgaacatcg actatcacca tggcatgctg
    11581 gttcctgcgg taggtgttca cagtatacag gtgcttcgcg ccaacaggga acatcccgac
    11641 tccgcggaag gttcaggata cacgtacaac catgccccga tgctggcata ctggaataac
    11701 aaattttatc tcgagtacct tagcgatagg gtaggggaga gcgttccgcc ggggcagacc
    11761 cttgtcacaa cgtccgaaaa cggttacaca tgggcgaaac ctgtggtcgt gttcccgcct
    11821 tacaagatcc ccgacggtac gacaaaagaa gggcatccgg gcgtagcgaa ggacctttat
    11881 gccgtcatgc atcaacgcat gggtttctat gtttcgaaag atcatcgcct gctggtgctt
    11941 ggattctatg gcatctgtct cgacccgcat gacgatccca atgacggcaa aggtatcggc
    12001 cgcgtagttc gcgaggtgca tgcggacgga agtttcggcc ccatctattt tattcattac
    12061 aaccccgcat ggactacggg aaatacttcg tatccccttt atacctccag caaggacaag
    12121 gattttgtcc gggattgcaa cgagctgatg gcgacaccgc tgatgatgat gcaatgggtg
    12181 gaggagacgg atcggaagga ccctcttata cccttgcata aggactataa ggcgttttgt
    12241 ttttatcatc tccccgatgg acacgtcgtc ggcatgtgga agaatggact caccggcacc
    12301 agcaaagacg agggccgtac atggtcgcgg gtcgtgcgtg cgccacatgt cgtcaccagc
    12361 aatgccaaga tgtggggaca acacacctcc gacgggaaat acgctaccgt ctacaatcct
    12421 tccgaatttc gctggccgct ggcgatctcg acgagcaaag acgggcttga ttataccgat
    12481 ctcctgctgg tgaacgggga gatcagcacg gcgcggtatg gaggtaatta caagaattac
    12541 ggaccgcagt atacacgggg gatcatcgaa ggcaatggaa ccccgccgga ccccaatatc
    12601 tggatcacgt atagcatgaa taaagaggac atctgggtga gcaccatccc ggtacctgtg
    12661 acggctgcgg ttgctgcgga tgtcaacgat gatttcggga aggcggattt gaaacagtgg
    12721 aatacgtaca gcccgcgttg ggcgcctgta ggtctggtcg atcataccct gcagctgcgc
    12781 gacgccgatc cctatgattt tgcaatggcc gagcgcgtct ttcccgtcgc caaaaagatg
    12841 caggtggagt ttagccttgc cgcaggtcaa aatgaccatg gacagctgca gatcgaaatg
    12901 caggatgagc acaatctgcc ggcaataagg ctgagcttcg agcccgacgg aacgctgatc
    12961 gccaaagcgg gcgcgcgcat caaaaagatc ctgagctatc tgccgggagt cgtctatacg
    13021 atacgtatca gtgtcgacgc ggatacaaga atgtataccg tgaatatcga cggaaaagat
    13081 gtactgacgc agctgttctt ccaacccgtc gaaagcttcg ggcatatcgt attccggacc
    13141 ggtgagaccc ggcatttccc taccgccgac tctcccgcgg aggcggatac ggacaggccc
    13201 aaagccggcg atcccgtgcc tatggccagc tattccattc gttatctgaa aactacccgt
    13261 gaatgagcgg gcgcgtattc ataggaatgg cgctgttgct ggccgcttct ttgaaggccg
    13321 gcgcgcaacg caatgtcgaa cgccccgtgc agccggagga tgttacggct atcggaggat
    13381 ttgcagggag tcgcattgaa aagaaccagc gcaactacat caaaaccttc gacatcgcga
    13441 aatatgtggg actcatggaa acgcgcgatt tcaccgggtg ggactggcgt cagggcgaac
    13501 agcccgggaa atggctggag gcggcgatat gggcttctac caggacacat gacacagcac
    13561 tctcgaacga agcacatatc atgctcgaca ggatcatccg cagtcaggcg cccgacggat
    13621 atatcggcat aaccagccag accgtccgca cgccggagcg tccgctggga ggcatggatg
    13681 cctatgagct gtatttcctc gaacatgctt tgctcacggc taacgacctg tggcacgatc
    13741 gcgcagccct tcgggcggcc cggcgtctgg gcgattattt cgtcagatac atcggacccg
    13801 gtaaggcgga attctggcct tcaccgctgc ggcccccgga aaacaaggac aaggttctca
    13861 aaggtaccat gcattccgag atcgcggggc atagcataca ctatggctgg gagggcacgc
    13921 tgttcatcga cccgatgatg cggctgtacc aggagaccgg tgaccgacgt tattacgact
    13981 ggtgcaaatg ggtggtgaac agcatcgata aatggagcgg ctgggacagc tattccaatt
    14041 tggacaaggt ggcggcagac tcgatgccca tcaacaagat acagccttat gtgcatgcgc
    14101 atacttttca aatgaacttc cttggactgc tgcgtatgta cgccgtcagc ggcgacacct
    14161 cctatctccg gaaggtggag ggcgcctgga aggatattgc atccagacaa ctgtacatta
    14221 ccgggggcgt cagtgtcggc gagcactacg aacgggatta tatcaaaccg ctcaccgggg
    14281 aaatgatgga gacctgcgcg acgatgtcct ggatgcagct gtcgcaggcg ctgctggaca
    14341 tcacgggcga cccgaaatat gcggatgcga tggggcggat cttatggaac aatgtctttg
    14401 ccgagcagac gatcgacggc gatgccaatc attattttac gccccccaat ggggtgacgc
    14461 cgcgcgggta ttatcgcgag cccgactgtt gtatgggcag cggcgaacga ctgctggcta
    14521 tgctgccggg attcatttac gcgaaaggcg cagggcggtc catctatatc aatcaattta
    14581 tcgcctcttc gaccgtgatt gacgggacca ggatcacaca aacgaccgat ttcccggaga
    14641 gcggaaaggt aacggtaagt gtggggccgg cgaaggctgg cgtgtttgcg gtttatgtgc
    14701 gtatccccgg ctgggctgac agcgcggtgg tggcggtaaa cggtgttgcg gtgcagaggg
    14761 ttcgcccggg cagctatctg aaactggagc gtacctggcg ttcgggggat aagatcggtg
    14821 tggagctgcc catgcagctt cattgggtga gacatgagca ttatgagaag acagcggacc
    14881 gcaaacccta taagaccgct ccggataacg atgcccccta tgcgctgttg cgcggaccgg
    14941 tagtatatgc gttggacgat atctggtata agggcgatac ggcggtattc cgcccgatgg
    15001 atagtatacg gtatgtgttg agtgatccgg acggcatgcg ccggatcggg gctccgggac
    15061 ccgatatcct ggggccgggg tatgaagtgc ggttgatatt ggccgatggg agtgagacat
    15121 cgattcctgt gtacccgttt gccaatatcg ggaaatggta tcgggatgcc gcgcacaaac
    15181 ccgacagcaa tgccgcggtt tggaagtatg ccgtttggct gaagggttcg gatgcggctt
    15241 cgggcaggct ttcgttgcca tctatcttcg gcgacaatat ggtgctgcaa cggaatgttc
    15301 cggtgcctgt gtggggggat gcggatgcgg gatcgaccgt gacggtgcat tttggctcgc
    15361 agaccaggag cgtggtggca gacggcaagg ggcattggcg tgttagtttg gggccactgt
    15421 ccgcttcgac cgaaggggtg gagatgacgg tgaccggaga tggggcatcg atccgtttcc
    15481 gggatgtgct ggttggtgag gtctggctct gcagcggaca gtccaatatg gaatatagta
    15541 tgcgtaagaa cagcaagttc gagaagggcg gcgatgccct cgatagcgcg cgcaatccgc
    15601 tgatccgcat ttttctcgtg cgcaacaact acagcaaacc tgacaggcat atccgccgtc
    15661 gctgggattc tgccgagggt gcaccgttgc gtgacttttc agcggcgggt tatttctttg
    15721 cgcaggaggt gttcaggagg ttgaacgtgc cggtaggcgt gatctcgaac gcgatccccg
    15781 gcagtcttat cgaaccctgg ctacccgatt ccggtaaatt ctataaaacc ctggtgaccc
    15841 cgctcgcgcc atatgccttg aaaggcttcc tgtggtacca gggtgaatcc aattgttttc
    15901 tcgccgatac gctgcgatac gcgggaaaaa tgaagacgct gatcgaaagc tggcgcaccc
    15961 tgtgggcgaa cccggacctg tccttttatt tcgttcagat agccccttat tattattcga
    16021 agacgacggg ttcgacgggg accgagcctg ttttctggga ggcgcaggaa aaagcattgt
    16081 ccctgccaca tacgggtatg gtcatgacga ccgacctggt agatacccca ttcgacctgc
    16141 acccggccta taaatggaag ataggtcgcc ggctggcgct gcttgcgctg gccaatgact
    16201 atcatgaagc ggtggtttgt tcgggaccgg tattcgacca cctgacggtc aggggtcgga
    16261 gggtagagct gcattttaca ggtggccccc ttgtcagccg caaacccctg aggtggttcg
    16321 agaccgaaga taaaagcggt aaattcactc ccgcaacggc ggccatcgcc ggtgacgaca
    16381 agatcatcct gaaagcccgt aaccggccga tcgctgtccg ttttggctgg aacgaagctg
    16441 cccaacccaa tttattcaat aaagaaggat tgcccgcgat gccattccgg gccacgacaa
    16501 atgaataagc tactatctat ctcgctgctc gccttgctgc ccgcctttgg ccaggggcag
    16561 gagactcaac gtgtttttct ttccgggacg ggtaacgacc ataccgtcga ctggcagttt
    16621 tattgcacgg aaggcaggaa ttcgggaaaa tggacaacca taccggttcc gtccaactgg
    16681 gagctccagg gtttcggcaa atacaactat ggcctggata aagattcggt ccgtggccat
    16741 gaaaaaggct tatataaata tcggttcact attcctgccg actggaaggg ccgtgaggtc
//
LOCUS       DASYSV010000062        12704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1549776, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000062 DASYSV010000000
VERSION     DASYSV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12704
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549776"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VGQ51_01865"
     CDS             <1..1045
                     /locus_tag="VGQ51_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV9035330.1"
                     /translation="LSVLLCQTAHAQVKGPAQTFIEKEVVFWDYHSTIIGDDYTIYVH
                     VPPGYDTSNVKYPSLYLTDGDWDKNPAINSFNMLRQDYITREAVIVAIGYGDGPNQRN
                     RDLEPEKGGPNFLAFIEKEVIPFIESKYRVTDDRTLSGYSYGGVFATYALFNRPGLFS
                     TVCIGAPGYSGRQLIPSARKYFATHKDLKARVYLGVGSYEHENVDNIAKFKTYLLQQN
                     CKDLEVETDVIPHAFHGAAKPPVIQNGVEFAFCKKHKPVTVPVADLEKYAGTYARAGK
                     PDNKIKFYVENGKLYISDGDTPIEFVPFTTDGGFFIYENQKADMYFKTEGGRMYELFV
                     PYNGSPVRFDRVD"
     gene            1127..1282
                     /locus_tag="VGQ51_01870"
     CDS             1127..1282
                     /locus_tag="VGQ51_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035331.1"
                     /translation="MRGEQTAGSCSGSSPSGPVKRAGWSRQLTAKDAQLYTIENGLGE
                     WGKKDYL"
     gene            1341..1862
                     /locus_tag="VGQ51_01875"
     CDS             1341..1862
                     /locus_tag="VGQ51_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9035332.1"
                     /translation="MENLAAIRNGDHAAFIRVYEQLHARVFRFFLKRVNFFDTAKDLT
                     QQCFIRVWQYRESLSLDHALEKQVFIIARGLLINHLKKEATQRKLKENVSRLTLERTE
                     PATDFHLERANEVNAAIEGLPKTRKRIIILKTFHGYSNKEIAQQLHISVKTVEDHVTK
                     AFRHIRQVIAFFL"
     gene            1926..2969
                     /locus_tag="VGQ51_01880"
     CDS             1926..2969
                     /locus_tag="VGQ51_01880"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEV9035333.1"
                     /translation="MPAITRELLEKFWMGFCTEEEATLVEAYFREHPEDESLFEEFEA
                     AGEEPVGDKHRVEMLSRVMHAVRPRGAVVRRIRVTMAAASLLAVVAAGWLIFRPAPGR
                     SVTGPAMSEAAVWVGKHNADNKNVRVMLPDSTETILAPDAIIIYRTDLGYYDKREVKV
                     EGEVVFMVRRNKEQPFIVYSGGVETTVLGTIFDVSNKKDGDHISIRLLQGKVMVGMDA
                     KTGDPAKKYYLRPGEEFVYKRGDRSVAVREFDHKGEAYAVHRARRPRMREDSLANWYM
                     FNDQELADVLDQLAIIYNVEIQYSSSDLRNKYFIGKLEKHDSLTEILKDIALLNHLSV
                     IGKEGCYIIRKAK"
     gene            2987..6253
                     /locus_tag="VGQ51_01885"
     CDS             2987..6253
                     /locus_tag="VGQ51_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9035334.1"
                     /translation="MEKFYLKALVLVLGMGFIAASLQAQRVRSGSVSGIVRNDQGEPV
                     VGVTVLATNLSTGTTAGTQTDTGGVFRFLKLPASGKYSFQFSSVGFVSQTLTGYSVKA
                     DGSTSILVRLEESKNSLNEVVVVGYGTQKQANLTGAVTQINREVLNNRSLPNLTQGLQ
                     GAIPNLNLVMADGKPYTSPVFNVRGSTSIGQGGAALVLIDGVEGDPSLLNPDDVASVT
                     VLKDASSAAVYGARAAYGVVLITTKSPKKGGFTVTWSSDYSLKMPTEIPHTVNNGYQY
                     ALMFDSAYYSWNDDAAVPQNVNKTQVFSAAYLTDLQKHDQDPSLPKVAVDPVTGQYVY
                     YSNTDWYGLLYKKNLGAIDQNLSISGGTGKTNYYVSGRYYNQDGLFRFNSDNYRMYNL
                     MAKGSMQVLPWLQIYNNTQFTARKYHNPINVGEGGGIWRNMADNSHTSTPLFNPDGSL
                     TSAGAYSVGDFWYGKNGIDFYDQIIKNTSGFVANIYKSKIQLRGDFTFQSTQSTQTQI
                     RVPVPYELSPGVVNYLGTNYNDIKNSYNPTSYLASNLYGQYENTFGSDHYVKVLVGYN
                     YEQSVLDGYSVQRNGLIFNDATNLNLALGQNTTTTGSYDKWDILGGFGRINYAFRDKY
                     LLEVDGRYDGSSKFPSNQRYAFFPSVSAGWRVSKEGFWHVPEKAISDLKIRGSYGSLG
                     NGNIASYNYQEQFKISQATRVLGGVLPQTTTAPGVVPAGLTWETAKTADVGMDLTMLS
                     GRLAVTADYYNRTTLNMFTVGPTLPNVFGATVPKGNYANMWTKGGELSITWHDQFKLS
                     GSAFHYHIGAWVSTYSAKVTRYNNATGILTDHYAGEKLGQIWGYVNDGYWTDANVGDA
                     KTYQSFIHVSNGNNYKPGDIRFRDLNGDKVVNNGANTVSNHGDMKIIGDSIPQYSFGI
                     NLGGDYKGFFLNAFFQGVGKMDWWPGAEADAFWGQYNRPYDFLMKSQVGKVWSESNPN
                     AYWPRYRGYVAQSTNRELYVAQTKYLQNVRYIRLKNIQIGYNLPQSLMSKIGLNGARV
                     YVSGENLWTASPLYKITRDFDVENIGKSDNIATGTTNNGNTNNYPILKSVTLGLSLTF
                     "
     gene            6299..8047
                     /locus_tag="VGQ51_01890"
     CDS             6299..8047
                     /locus_tag="VGQ51_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9035335.1"
                     /translation="MMLLTAGALASCTKNLDQTPQSTATEASVFGSQTGLALYSNSFY
                     NILPDIKTPFRTDCNLSDYGAVSSVPLYIQQGAYTSLNNNDWTTSIWGDLRSINYFIA
                     HCDNPGVPASVNANYIGLAKFFRAYFYYRKVVEYGDVPWIGKALDVSDTTILYASRTP
                     RNVVMDSVLADLDYAIAHITATNDPTRSTITRWVAYGMKSRICLFEGTYREYQEQDKA
                     SATTWLQQAAAAAKAVMDSAGFSLNTGGNMAYRNLFISPTPLTNEVMLADVTSTSLGV
                     YTDANWYLTSATYGPRFNFIRTFINTYLNIDGTRFTDIPGHDTISFPNETKNRDWRLQ
                     QTIRTPGYTRISSGNVVPSPPVFSYTYTGYQPIKWVLDDMVNDNGATNTNSICLMRYA
                     EILLNYAEASEELGTFADADWNKTIGALRARAGITGGINTKPAVADPYLQAHYFPDIT
                     DATLLEIRRERGIELALEGFRLDDLIRWKHGELLLNTWNGFYVPALNTLMDLNADGKP
                     DVYFYQGSAPANKVSGVTYVNVSGPGLTLSSGTYGELHWLDNVPRQWFDWMYVNPIPY
                     SALELNPKLQQNPNWQ"
     gene            8059..9351
                     /locus_tag="VGQ51_01895"
     CDS             8059..9351
                     /locus_tag="VGQ51_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEV9035336.1"
                     /translation="MQKILFLMTTLLAVLAGRVFAQGGRPIRHVVVVTIDGSRPDFYL
                     DSSWHAVHLRELAASGTYARGVNSVFPSMTYPSHTTIVTGVQPAKHGVYYNTGPQGIY
                     WNDSSIKSPTLWQAAEDKGMVVASLFWPVSADAPVQYNIPDIGSLGEAVREKYSKPAG
                     FVDTLKAEVFGGASRIEYGRDVNVAKIAANVIGKARPNLMTIHLFSVDHNEHMEGRDG
                     PGVRAAVRGADSAVGIIMDALKAAGIWDSTVLIVTGDHGFMTVHTQVNPNVWLAKAGL
                     ITDLKKGDWKAMFFSVGGSSYLYSKDATALASVKRILRELPDSVKNYVRVIDRRQLDK
                     IGGNPEVALALSGLNGAAFGNATAGDAVRPAHGGQHGFFPDFYEIRTGFVIHGPGVRV
                     GGVIPEMNERDIAPTVARLMGIPFPSAQGKIPVGVLTE"
     gene            complement(9476..10222)
                     /locus_tag="VGQ51_01900"
     CDS             complement(9476..10222)
                     /locus_tag="VGQ51_01900"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEV9035337.1"
                     /translation="MRSLMAVLVAGSISAMPNPTHAQTRAVGHFYKVIVSPYIQATFV
                     EGSEESVTVNSTIVDSDKLHVEVHGGELRLYLDGAKDLPHYQRDYREDDGQQSHPLYP
                     NHAVIVTVVYKKLDALSLRGTETYLCESPLSEKKFTLRVYGEGNVIFTEVHLSKMHTI
                     LYGESCLDIRSGEANEQYYTCYGEGKVNSTAIAGQAAKVTAFGEAEFKVNVSDVIRVT
                     SFGEARVCYLGNPTIIKALHFGGVDLRRLN"
     gene            10317..12185
                     /locus_tag="VGQ51_01905"
     CDS             10317..12185
                     /locus_tag="VGQ51_01905"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV9035338.1"
                     /translation="MIKTFIFSTILFLAFTRTDAQTLTMPPEIQKAYDNGTRSLNGKP
                     GKNYWENHGHYHISLTVAPPNTLITGVEHITYYNNSPDTLKSLNMKLIENVHRGGTVR
                     GGVADTAAAIKVDNMTINGAQTAWDNEKAITTNQMVDLPAPLMPHDSIKLDITWHYNL
                     SKRRGREGVIDSTSFYVAYFYPRVSVYDDYKGWDTQPHTGSLEFYNDFNNYEVEVTVP
                     KNFIVWATGTLVNPSEVLQPEAAQRLEQSFTSDSTIHVATRQELADYKVTAQNATNTW
                     KWSAENVSDVAIGISDHYDWDAGSVVVDNKTQRRASMQAAFADSSDDFHHSVQFGRYS
                     LAWFSNNWPGVPYPYSKSNAFQGFADMEYPMMINDSHAPDLGFAELVQDHEQAHTYFP
                     FYMGTNESRYAFMDEGWATTFEYLIGISEKGQAAADQFYKSFRVTRWIHGPHEKENPI
                     ITPSPDVTFGAGNNAYGKPSLSYLALKDLLGDKLFKKGLHNYMENWNGKHPIPWDYFY
                     SMSKGSGKDLNWFFYNWFFTPGYIDLDLSNVQKAGKGYTLSITNVGGFAVPFDVVATY
                     DDGTTERFHQTPKVWERDQKATSVTVKTGKAVKALALDGGIFMDANEKDNTWMAQP"
     gene            complement(12256..>12704)
                     /locus_tag="VGQ51_01910"
     CDS             complement(12256..>12704)
                     /locus_tag="VGQ51_01910"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEV9035339.1"
                     /translation="INDSKAAKASFIKSDASMENLFKHSAGYVIFPNVGKGAAGLGAA
                     AGQGAVYDKGVLIGTAHMTQVTVGAQAGGQAYREIIFFENQDALDRFMHDKVEFSGQA
                     SAIAVKAGASANANYRNGVVVFSQEKGGLMLEASLGGQKFAYKTLK"
BASE COUNT         3097 a         3139 c         3500 g         2968 t
ORIGIN      
        1 cttatcggtc ttactctgcc aaaccgccca tgcccaggtg aagggccccg cccaaacatt
       61 catcgaaaag gaagtggtct tctgggatta tcattctacc atcattggag acgattatac
      121 gatctatgtc cacgttcccc cgggatacga taccagcaac gtgaaatatc catccctata
      181 cctgacggac ggcgactggg acaagaaccc agccatcaac agcttcaata tgctgcgcca
      241 ggattacatc acgagggaag cggtcatcgt agccatcggt tatggcgacg gtcccaacca
      301 gcggaacaga gacctggagc cggaaaaggg cggtccgaat ttcttagcct ttatcgaaaa
      361 agaggtcatc ccgttcatcg aaagcaaata ccgggtgacg gatgacagga cattgagcgg
      421 ttattcttat ggcggggtgt ttgctaccta tgcgcttttt aaccgtcccg gtttgttcag
      481 tacggtttgc ataggagctc ccgggtatag cggtcgccaa ttgatacctt ccgcccggaa
      541 atactttgcc acacataagg atctcaaggc aagagtctac ctgggagtgg gctcctacga
      601 gcatgagaat gtcgacaaca tcgcgaaatt caagacttac cttctgcaac agaactgtaa
      661 agacctggaa gtcgaaaccg atgtcattcc tcacgctttt catggagcgg ccaaaccgcc
      721 ggtcatccaa aatggagtgg aattcgcctt ctgcaaaaaa cataaacccg ttaccgtgcc
      781 tgttgccgat ctcgaaaaat acgcaggcac ttatgcacgc gccggaaaac ccgataacaa
      841 gatcaaattc tatgttgaga acggcaagct ttatatttcg gatggcgaca caccgataga
      901 attcgtcccc tttacgactg acggcggctt tttcatctac gagaaccaaa aggctgatat
      961 gtactttaag acggaaggcg gcaggatgta tgaattattc gttccgtaca atggaagccc
     1021 ggttcgtttc gacagggtcg attaagccgc tctgctccaa taaccccacc tcgcagctgc
     1081 ctgctggtca cgcggtcgaa attcgaccgg ctcataaaac cctgatatgc ggggggagca
     1141 aactgcggga agctgttcgg ggtcctcccc gagcgggccg gtcaagcgag caggttggtc
     1201 gaggcaattg acggcgaagg atgcgcagct gtatacgata gagaatgggt taggagaatg
     1261 ggggaagaag gattatcttt gagttaaggc tctggctggg agctgactgc tttggcgatt
     1321 ttttgcaact tgcgaaccat atggaaaatc tagctgctat ccgaaacggt gatcatgctg
     1381 ctttcatccg ggtgtatgag cagctgcatg cgagggtgtt ccgcttcttt ctcaagagag
     1441 ttaatttttt cgacacggca aaagatctta cgcaacaatg ttttatccgg gtttggcagt
     1501 accgggaatc gctttcattg gaccatgcat tggagaagca ggtctttatc attgcccggg
     1561 ggttactgat caatcattta aagaaggagg caacgcagcg gaagctaaag gagaatgtga
     1621 gccggcttac actggagaga acggaaccgg cgactgattt tcatttagag cgggccaatg
     1681 aagtgaatgc cgccatcgaa ggtcttccga aaacgcgtaa gcggatcatt atcttgaaaa
     1741 cttttcatgg ctactccaat aaagagatcg ctcaacaatt acacatctcg gtaaagaccg
     1801 tcgaggatca tgtaacgaaa gcattccggc acatccggca ggttattgcc ttttttttat
     1861 aactgcaggg gcgagcaggg gtagagctat ccttgaactg tacatagtta tcgaataaac
     1921 catcaatgcc agcaattacc cgggaactct tggaaaagtt ctggatgggc ttttgtaccg
     1981 aagaagaggc gacgctcgtc gaagcttatt tcagagaaca tccggaagat gagtcgctat
     2041 tcgaggaatt tgaggcggcc ggcgaagagc ctgtggggga caaacaccgg gtagagatgt
     2101 tgtcgcgggt gatgcacgcc gtccggcccc ggggggcagt ggtccggcgg ataagggtaa
     2161 cgatggcggc ggcttcgtta ctggcggtcg ttgcagcagg gtggttgatc ttccggccgg
     2221 cgcctgggcg aagtgtgacg ggacccgcga tgtcggaggc tgcggtttgg gtgggaaagc
     2281 acaatgcgga caataaaaat gtacgggtca tgctgccgga cagtactgaa acgatcctgg
     2341 ctcccgacgc cattatcatc taccgaacgg atcttgggta ttatgataag cgggaagtga
     2401 aggtagaggg cgaggttgtt ttcatggtaa gaagaaacaa ggagcagcct tttatcgtct
     2461 acagtggcgg tgtggaaacc acggttttgg gcaccatttt cgatgtttca aataagaagg
     2521 acggcgacca tatcagtatc cggttgctgc agggtaaggt catggtggga atggatgcga
     2581 agaccggtga tcctgcaaaa aaatattacc tgagaccggg agaagaattc gtgtacaagc
     2641 gcggcgacag gagtgttgcg gttcgggaat tcgatcataa aggggaggcc tacgccgtgc
     2701 atagggcgcg tcggccgcgg atgcgggagg acagtcttgc gaactggtat atgttcaacg
     2761 accaggaact cgcggatgtg ctcgatcagt tggctatcat ctataatgtc gagatacaat
     2821 attccagttc tgacctgcgt aacaaatatt tcatcggcaa actggaaaag catgattctt
     2881 taaccgagat cctcaaagat atcgcgctat taaaccatct atccgtgatc gggaaagaag
     2941 gatgttacat catccgaaaa gccaagtaaa cgcaaactaa cacataatgg aaaaattcta
     3001 cttgaaagca ctcgtgctgg tgcttggcat gggatttatt gccgcgtccc tgcaagcgca
     3061 gcgagtccgc tcgggaagtg tcagcggcat tgtgagaaat gaccagggcg agcccgtggt
     3121 cggggttact gttctggcga cgaatctttc gaccgggacg acggccggaa cacagaccga
     3181 cactggcggg gtgttccgtt ttctaaaatt acctgccagt ggcaagtaca gttttcagtt
     3241 cagtagtgtg ggttttgtca gtcaaacgct taccgggtat tcggtgaaag ccgatgggtc
     3301 cacttccatt ctcgtgcgat tggaggagag taaaaactcg ttgaatgagg tggtggttgt
     3361 aggttatggc actcagaaac aggcgaacct tacgggcgcc gttacgcaga tcaatagaga
     3421 ggtgttgaac aacaggagcc tgccgaattt gacccagggc ttgcaggggg ccattccgaa
     3481 cctgaatctc gtgatggcgg atggtaagcc ttatacttcc ccggtcttta atgttcgcgg
     3541 aagcacttcg atcgggcagg gtggcgccgc gcttgtgctg atcgacggtg tcgagggcga
     3601 cccgagtttg ctgaacccgg atgacgtcgc cagcgttacc gtattgaagg atgcgtcttc
     3661 ggcggcagtc tatggtgcga gggctgccta tggcgttgtg ctcattacga cgaagagtcc
     3721 caaaaaaggc ggtttcaccg tcacctggtc ctccgattat tcgctgaaaa tgccgaccga
     3781 gatcccgcat acggtgaaca atggttatca gtatgcgctg atgttcgact ccgcttatta
     3841 ttcctggaat gacgatgcgg cagtaccgca gaatgtgaac aagacacagg tgttctcggc
     3901 cgcatacctg accgatctgc agaagcatga ccaggacccg agcctgccga aggtagcggt
     3961 cgatccggta acgggacaat atgtctacta ttccaatacg gactggtatg ggttgcttta
     4021 taaaaagaac ctgggcgcta tcgatcagaa tctttccatt tcgggcggca ccggcaagac
     4081 gaattattat gtatccggcc ggtattataa ccaggacggg cttttccgat tcaattccga
     4141 taattaccgg atgtacaacc tgatggccaa gggcagcatg caggtgctcc cgtggctgca
     4201 gatctataac aatacccagt tcaccgcccg aaagtatcac aaccccatca atgtgggtga
     4261 aggcggcggc atctggcgta atatggccga caactctcat acttctaccc ctcttttcaa
     4321 tccggacgga tcgctgacgt cggcgggcgc ttattcggtc ggggatttct ggtatggtaa
     4381 gaacggcatc gatttctatg accagatcat caagaataca tcgggattcg tagcgaacat
     4441 ctacaagagc aagatacagc tgagaggcga ttttactttc cagagcacac agtctaccca
     4501 gacccagatc cgcgtgccgg tgccctatga gctttctccc ggcgtggtca actatctcgg
     4561 tacgaattac aatgatatca agaactcgta taacccgacg agttatttgg cgtcgaatct
     4621 ttatgggcag tatgaaaaca ccttcgggtc ggaccattac gttaaggtcc tggttggata
     4681 caactatgag cagtcggtgt tggatggtta tagtgtacag cgcaacggtc ttatattcaa
     4741 cgacgccacg aatttgaacc tcgcgttggg acagaatact acgactaccg gtagctatga
     4801 caaatgggat atccttggcg ggtttggccg gatcaactat gcattccgcg acaaatatct
     4861 gctggaagtg gacggccggt acgacggttc ctcgaagttc ccatccaatc agcggtatgc
     4921 gttcttccct tccgtgtcgg ccggctggcg tgtctccaag gaaggtttct ggcatgtgcc
     4981 tgagaaggct atctccgatc tgaagatccg cggttcatac ggttcgcttg gcaatggaaa
     5041 tatcgcctca tataattacc aggagcagtt caagatctcg caggcaacgc gggtgctggg
     5101 cggtgtattg ccgcagacca ctactgcgcc gggcgtggtt ccggcgggtc ttacctggga
     5161 gacggccaag acggcggatg tcggtatgga cctgaccatg ctttcgggcc ggctggctgt
     5221 tacggccgat tactataacc ggacgacgct gaatatgttc acggttggcc cgacgctgcc
     5281 gaatgtcttt ggcgcaacgg taccgaaggg caactatgcc aatatgtgga ccaagggtgg
     5341 agagctttcg atcacctggc acgatcagtt caaattatcg ggctccgcct tccactatca
     5401 catcggcgca tgggtgtcga cctattcggc gaaggtgacc aggtacaaca acgccaccgg
     5461 catattgacc gaccattatg cgggcgagaa gctcggccag atctggggtt atgtgaacga
     5521 tggttattgg acggatgcca atgtgggcga cgcgaagact taccaaagtt tcatccatgt
     5581 ctccaatggc aacaactata agccgggaga tatccggttt agggacctga atggcgacaa
     5641 ggtggtcaat aacggcgcca ataccgtgag caatcacggc gatatgaaga tcatcggcga
     5701 ttccattccg caatacagct ttggcatcaa cctggggggc gattacaagg gattcttcct
     5761 gaatgccttt ttccaggggg tagggaagat ggattggtgg cccggcgcag aggcagatgc
     5821 tttctggggt cagtacaacc ggccttatga tttcctgatg aagtctcagg tcggcaaggt
     5881 ctggtcggaa tccaatccga atgcctactg gccgcgttac cggggttatg tcgcgcaaag
     5941 tacgaaccgc gagctgtatg ttgcccagac caagtacttg cagaacgtgc gttatatccg
     6001 actgaagaat atccagatcg gttacaatct gccacagagc cttatgtcga agatcggact
     6061 caatggcgcc agggtctatg tctcgggcga gaatttgtgg accgcgtcgc cgttgtacaa
     6121 gatcaccagg gacttcgatg tggagaacat cggaaaatcc gacaatatcg cgacgggtac
     6181 gaccaacaac ggtaatacga ataactatcc tattcttaag agtgtaacac ttggcttatc
     6241 tctcaccttt taaaaacaag ttatgaccct tcatcgttct atcataaaaa acgttgttat
     6301 gatgctgctg acagcggggg ctctggcttc ctgtacaaag aatctcgatc agactccgca
     6361 gagtacggcc accgaggctt cggtgttcgg gagtcagacc ggtttggcgc tttattccaa
     6421 ttccttttat aatatccttc cggatatcaa gacacctttc cggacagatt gcaacctttc
     6481 cgattacgga gcggtgtcga gtgtgcccct gtatattcag cagggcgcct atacatcatt
     6541 gaataataac gactggacta catcgatttg gggtgacctg cggagcatca actattttat
     6601 tgcgcattgc gacaatccgg gggttccggc ttcggtgaat gccaattata tcggtttggc
     6661 gaagttcttc cgcgcttact tctattacag gaaggtggtc gaatatggcg acgtgccgtg
     6721 gatcggcaag gcgctcgatg ttagcgatac cactattttg tatgcttcgc ggactccgcg
     6781 gaatgtggtg atggattcgg tgctggccga tctcgactac gccattgccc atattacggc
     6841 taccaacgat ccgacccgca gcacgatcac caggtgggtt gcttatggca tgaagtcaag
     6901 gatctgtttg ttcgaaggca cctatcgtga gtaccaggag caggataaag cgtcggctac
     6961 tacctggctg cagcaggcgg ccgcagcggc aaaggcggtg atggacagcg ccggattcag
     7021 tctgaacacg ggcggcaata tggcgtatcg caacctgttc atcagcccga cgccgttgac
     7081 caatgaagtg atgctggccg atgtgacgag tacctccctg ggcgtctaca ccgatgccaa
     7141 ctggtatctt accagcgcca cgtatggccc gcggttcaat tttatccgga cgttcatcaa
     7201 tacgtatctg aacatcgacg ggacacggtt caccgatatt ccgggacacg atacgatcag
     7261 tttcccgaac gaaacgaaga atcgcgactg gcggcttcag caaactatcc ggaccccggg
     7321 ttatacccgt atcagcagtg gtaatgtggt accgtctccg cccgtatttt cctatactta
     7381 tacgggttac cagcctatca agtgggtgct cgatgatatg gtgaacgaca atggagcgac
     7441 gaataccaat tccatctgtt tgatgcggta tgccgagatc ctgttaaatt atgcggaggc
     7501 cagtgaagag ttggggacct ttgccgatgc cgattggaac aaaacgatcg gtgcgcttcg
     7561 tgcacgggca ggcattaccg gcggaattaa tacgaagcct gcggttgccg atccttattt
     7621 gcaggcgcat tattttcccg atatcacgga tgccacattg ttggagatcc ggcgggaacg
     7681 agggatcgag cttgcgctgg aaggtttccg gctcgatgat ctgatccggt ggaaacatgg
     7741 cgagctgctg ttgaacacct ggaatggatt ctatgttccg gccctgaata cgctcatgga
     7801 tctcaacgca gacggaaaac ccgatgttta tttctaccag ggttcggctc ctgcgaacaa
     7861 ggtaagcggc gtgacgtatg tcaatgtcag cggccccggt ctgacgctgt ccagcggcac
     7921 ttatggcgag ctgcattggc tggacaacgt accacggcaa tggttcgact ggatgtacgt
     7981 caatccgata ccttacagcg ctctcgagct caatcccaag cttcagcaga atcccaattg
     8041 gcaataatca atctgaatat gcaaaagatc ttgtttttga tgacgaccct gctggctgtt
     8101 ttggccggca gggtttttgc gcagggcggc cgcccgatca gacatgtcgt ggtcgtcacg
     8161 atagacggtt ccaggcccga tttttatctc gacagcagct ggcacgcggt acatctccgg
     8221 gaactggcgg cttcgggcac gtatgcccgc ggtgtcaaca gcgtttttcc atccatgacg
     8281 tatcccagtc ataccacgat tgtgacggga gtgcagcctg cgaagcatgg cgtttattat
     8341 aacaccggac cgcagggtat ttactggaat gacagttcga tcaagtcgcc cacgctgtgg
     8401 caggcggcgg aggacaaggg gatggtggtc gcttcgcttt tctggccggt gagcgccgat
     8461 gcgccggtgc agtacaatat tcccgatatc gggagcctgg gtgaggcggt acgcgagaaa
     8521 tacagtaagc cggcgggttt tgtagatacg ctgaaggcgg aggtttttgg cggtgcgtcg
     8581 aggatcgagt atggcaggga cgtgaatgtt gcgaagatcg cggctaatgt gatcgggaag
     8641 gcacggccga acctgatgac gattcacctg ttctcggtag atcacaatga gcatatggaa
     8701 ggccgcgatg gccccggggt gcgggcggcg gtgcgcgggg cggacagtgc ggtgggcatc
     8761 atcatggacg cgttgaaggc cgccggcatc tgggacagca cggtgttgat cgtaacgggg
     8821 gatcacgggt ttatgacggt gcatacacag gtgaatccca acgtatggct ggcgaaggcg
     8881 ggtctgatca cggatctgaa gaaaggggac tggaaggcga tgttcttttc ggtgggcggc
     8941 tcttcttatc tttattcgaa agatgcaaca gcgctggctt ccgtgaagcg tattctacgc
     9001 gagctaccgg attccgtgaa aaactatgta cgcgtgatcg accggcggca gttggacaag
     9061 atcggtggca atcccgaggt ggcgctggcg ttgagcggat tgaatggcgc agcattcggg
     9121 aatgctaccg ctggggacgc ggtgcggccg gcgcatggcg ggcagcacgg attcttcccc
     9181 gatttctatg agatcaggac gggttttgtg atccatgggc cgggggtgcg cgttggcgga
     9241 gtaatcccgg agatgaatga gcgggatatt gcgccgacag tggccagact gatgggcatc
     9301 cccttccctt ccgcgcaggg caagatcccc gtcggtgtgt tgacggagta aaaccattgc
     9361 cgctttccgc gtcgcttgcg ggcaggcgcg ccggcggcgc ccaggctggt gccggtgcaa
     9421 ggaatgatac agttgaaaaa ggtgaattgt gagtcgcatc gacataagga agaagtcagt
     9481 tcagtctccg caagtcaacc ccgccaaaat gtaaagcctt tatgatggtc gggttgccca
     9541 ggtaacaaac cctggcctcg ccgaaagagg ttaccctgat gacgtcggag acatttacct
     9601 tgaattcggc ttcgccaaaa gcggtgacct tagccgcctg tccggcaata gccgttgagt
     9661 tgaccttccc ctctccatag caggtataat actgttcatt ggcctccccg gagcggatat
     9721 ccaaacaact ctcgccgtac aggatagtgt gcattttgga aaggtgtact tcggtaaaga
     9781 ttacgttccc ttctccatat acacgaagag taaacttttt ttccgacaaa gggctctcgc
     9841 aaagataggt ttccgtcccg cgcagcgaca gtgcatccag ctttttgtaa acgaccgtga
     9901 cgatcactgc atggttggga taaagaggat ggctttgctg gccgtcgtcc tcccgataat
     9961 cacgctggta atgcggaagg tccttcgccc cgtcgagata caaccgcaat tcgccgccgt
    10021 gcacctcgac gtgcagcttg tcgctgtcga ctatagtgct gttgacggta acgctctcct
    10081 cgctgccttc gacaaaagta gcctggatat acgggcttac gatgacctta tagaaatgtc
    10141 cgacagccct tgtttgtgca tgggtcgggt tgggcatggc cgaaatcgac cccgcgacca
    10201 aaacagccat taatgatctc atatgtatcg ttttatcgtg atacgaacga gccgggcaat
    10261 tgttacaggg aaaaccatac atttacccca taatttatcc aaatttaaaa ctccgcatga
    10321 taaagacatt catcttctcc accattcttt tcctggcatt tacccggacc gacgcccaaa
    10381 ccctcacgat gcctcccgag atccagaagg cttacgacaa cggcacccgc tcgctcaacg
    10441 gcaagcccgg aaaaaattac tgggaaaatc atgggcacta tcacatttcc ttaaccgtcg
    10501 caccgccgaa taccctgatc accggagtag agcacatcac ctattataac aatagcccag
    10561 atacgctgaa aagcctgaat atgaaactga tcgaaaatgt ccatcgcgga ggtaccgtcc
    10621 gcggaggagt cgccgacacg gcagccgcga tcaaggtcga caacatgacg ataaatggcg
    10681 cccaaacggc atgggacaat gaaaaggcga ttacaaccaa tcaaatggtc gatctgccgg
    10741 cgccgctgat gcctcatgat tcgataaagc tcgatattac ctggcactac aatctatcca
    10801 aaagacgcgg tcgtgaaggc gtgatcgatt ccaccagttt ttatgtcgct tatttttatc
    10861 cgcgggtatc cgtatacgat gattataaag gatgggacac acagcctcac accggttcat
    10921 tagagtttta taacgatttc aacaactatg aggtagaagt gacggtgcct aagaatttca
    10981 ttgtctgggc gaccggtaca ttggtgaacc cgtcagaagt cctgcagcct gaggccgctc
    11041 aacgcctcga acaatccttc accagcgatt ctacgataca cgtcgctacc cggcaggagc
    11101 tggccgacta caaagtcact gcccaaaatg ccacgaatac ctggaaatgg tccgccgaga
    11161 atgtaagcga tgtggcaatc ggcatcagcg accattatga ttgggatgcc ggaagcgtcg
    11221 tcgtggataa caaaacgcag cgacgtgcca gcatgcaggc cgcatttgcc gactcgtcgg
    11281 acgattttca tcattcggta caattcggca ggtattcttt ggcctggttc tcgaacaact
    11341 ggccgggcgt accctatcct tactccaaat caaacgcatt ccagggattt gcggatatgg
    11401 aatatcctat gatgatcaat gacagccatg ctcctgacct gggctttgcc gagttggtac
    11461 aggatcatga acaggcgcat acctacttcc cgttttatat gggcaccaat gagagtcgtt
    11521 acgcctttat ggacgaagga tgggccacca ctttcgaata ccttatcggc atttcggaaa
    11581 aaggacaggc agcggccgat cagttctata aatcgtttag ggtaacgcgc tggatccatg
    11641 gcccgcatga aaaggaaaat cccattatca ccccgagccc ggacgttacc ttcggcgccg
    11701 gcaataacgc ttatggcaaa ccgtccctga gctatctcgc cctgaaggat cttctcgggg
    11761 ataagctatt caaaaagggg ctgcataatt atatggaaaa ctggaatggg aaacacccta
    11821 ttccctggga ttatttttat tcgatgagca aaggctcggg caaggatctg aattggtttt
    11881 tttataactg gttctttacg ccgggctata tcgacctgga cctgtcgaat gtccaaaaag
    11941 ccggtaaggg atatacgttg tccatcacga atgtaggtgg atttgccgtt ccgttcgacg
    12001 tggtcgcgac ttatgacgat ggcaccaccg aacgctttca ccagaccccg aaggtttggg
    12061 agcgtgatca aaaggcaacg agcgtaaccg ttaaaacagg aaaagcggtg aaggcccttg
    12121 cattggatgg gggaatattt atggatgcca atgagaagga taatacatgg atggctcagc
    12181 cataggttac aacgataccg acgatgaata aaaaaagccg ctgaaattca gcggctttcc
    12241 tatttgtcag cttacttatt tcaacgtctt ataagcgaat ttctgtccgc ctaaggaggc
    12301 ttccagcatc aatccgcctt tttcctgcga aaatacgacc acgccatttc tatagttggc
    12361 attggccgat gcacccgcct tgacggctat cgccgaagcc tgtccgctaa attcgacctt
    12421 atcgtgcatg aaacgatcca gtgcgtcctg gttttcgaag aagataattt cgcgatatgc
    12481 ctgtccgccc gcctgggcgc cgaccgttac ctgtgtcata tgagcagtac cgatcagaac
    12541 tcctttgtca tatacggcgc cctgaccggc agctgcgccc aggcctgccg cacccttgcc
    12601 tacattagga aagatgacat agcccgctga atgtttaaac aaattttcca tagacgcatc
    12661 tgactttata aaggaagctt tggcggcttt actgtcattg atta
//
LOCUS       DASYSV010000063         7187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1679438, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000063 DASYSV010000000
VERSION     DASYSV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679438"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /locus_tag="VGQ51_01915"
     CDS             complement(<1..1813)
                     /locus_tag="VGQ51_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9035340.1"
                     /translation="MLVTRKLTFALFFFLSLATTAIGQSKFHVEGKVKQSTAEPLAGA
                     TIAEKGASTSTLTREDGSFTIDVSGPNATLVISYVGYTSKEIKLNGQQRLDITFQSTP
                     SNLDEVVVIGYGTTTRKDLTGSVASVSGKTISAIPVPNIAQAMQGKLPGVSVVSQDGR
                     PDADVEIRVRGGGSISQSNQPLILIDGVPGTLSDIPPDQVKTIDVLKDASSTAIYGAR
                     GANGVVLVTTKGAQEGKTRISYNGYVKFNTPAKYLGELAPYDYLKYVWANAAANGAAY
                     QTPFEQLFGLGANAGKNTGGIESYRTLQTDNEQKLVYNNSTSSNHDLTITGGTDRTKM
                     LFSVNYANEQGMKINSYLKRANVSFKLSQKLFDNLIFNLDTRYTDIQDMDDEGTTNGS
                     GSILSTAYRFRPIATNHILGDTNALKTGNIEQYGKNAMWDLYSPAARIADFNPLSISQ
                     GARAIASLDWKIIKGLVYHTDLTLSRSWGQKRYWSGAVYNNYVDDAGNHLFAGAVDYG
                     KSDSWGMRWSNTLNYDFIINSNNRLNLLAGQEVSNSGGSSMDIQANHFPSNFDKTTAF
                     AQINQYDQVNGTSQFSSSVTIPSRLLSYFGRANYSLMD"
     gene            2534..2788
                     /locus_tag="VGQ51_01920"
     CDS             2534..2788
                     /locus_tag="VGQ51_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035341.1"
                     /translation="MEMPLNSLSGSVLRKLALNELRQFIRLLDDGTTAELQSKKAYLT
                     AIFAKLSEKEQEEFHRLIYLVGNVDTSFADAGPAPLQKSA"
     gene            3214..3486
                     /locus_tag="VGQ51_01925"
     CDS             3214..3486
                     /locus_tag="VGQ51_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035342.1"
                     /translation="MKKSGMGVRLTARILQEWKDLGFRFVLVKGYTVDRYAGCIELNH
                     FMVEPVKQLPEDPADQEIYEPIDSEILLGWASSTESGVLAFIQKDS"
     gene            3491..3946
                     /locus_tag="VGQ51_01930"
     CDS             3491..3946
                     /locus_tag="VGQ51_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEV9035343.1"
                     /translation="MCAENQKNKPGYWRSLFHHKCSRCRTGDMFKVRSSYNLRQFMKM
                     YDKCPVCDQDLEIEVGFYYGTSYISYAITVALSAVTCLAWWVSIGFSLNDGRFFWWMG
                     CNIVALVIAQPWLMRFSRAIWLSFFVRYNENWQVERPADPERKGSRKPD"
     gene            3978..4352
                     /locus_tag="VGQ51_01935"
     CDS             3978..4352
                     /locus_tag="VGQ51_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035344.1"
                     /translation="MASNAMEQYTNGKNEANTAEGCDAEPYITPMKNEHNDNIHAAVP
                     LTLHTLKELKNQGFRYVLVNAFSHGRCPDYIEPSYFMLSPLMHLSEDREQKGIYESID
                     SQILVDWANTPNEGIKVFITKG"
     gene            complement(4363..5115)
                     /locus_tag="VGQ51_01940"
     CDS             complement(4363..5115)
                     /locus_tag="VGQ51_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035345.1"
                     /translation="MLDNFKILLHQGKQYIPDVTKAQVPGNFRGRPVISTSPVDSHAL
                     AELCPTGAISGDPVSIHLGKCTFCGECAFSFPEKICFTRDYRIATNLRNELIVREGVD
                     VPIRLNPDAIREEISKYFSGSLKLRQVCAGGDSSTEWELNACGNVNFDMGRFGVEFVA
                     SPRHADGIVITGPISENMAEPLRICYEAIPEPKIIILAGTDAISGGIFAGRPSLNRGF
                     LQNYKIDLYVPGNPVHPLTFINGVLDLIRKRK"
     gene            complement(5128..6627)
                     /locus_tag="VGQ51_01945"
     CDS             complement(5128..6627)
                     /locus_tag="VGQ51_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035346.1"
                     /translation="MNRISISNNQTIPLRDIPVLDYDDFLEMNIKMLERAESHCVNYY
                     GFRWKHGIKLICCIAHDREHSIHLSSAEISTHGGALPSFTARNLAFQGFERELAENFG
                     LSYTDHPWLKPLRYAFNRTDRSKTIANHPFYRIDSEELHEVGVGPIHAGIIEPGHFRF
                     ICNGEQILHLDIQLGYQHRGIESLLVSRKKPLERVTIAESIAGDTVVGHSLAFVNLWE
                     SLSGFDPPEELQLSRTLALELERMAIHTGDLSAICTDIAYQLGSAVFGRLRTPLINFM
                     QEWCGNRLAKGLIRVGGSHFPFNGSLAQRLGHLLDAYEPALTEMFDEMKSLPTVLARL
                     EKTGVVTREQAASIGAVGMVARTSGIDRDIRESHPFGLFRRTVHQPIIKHHGDVYSRT
                     QLRKEEIAQSTRYAREWLRNIPVPATRTDAPGRPAAGAFAISLVEGWRGEICHCAVTD
                     DAGELLHYKVKDPSFHNWLAVALAVRNNEISDFPICNKSFNLSYCGHDL"
     gene            complement(6632..>7187)
                     /locus_tag="VGQ51_01950"
     CDS             complement(6632..>7187)
                     /locus_tag="VGQ51_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV9035347.1"
                     /translation="FAYSSVEHIGLVTLGLAAGGVGYYAAILHIVLHSFAKSAMFFQV
                     GQVYRIYKSKSIYDAGDYFRFNPAGAMVLLAGFFVITAMPPSGLFISEFLLFSALFAG
                     GHFALLAAVLLLLTVIIWCFGKNIFKILFIKPVGFDEGAVERIPPVESLSQYVLLAGV
                     VYLGFFPPGVFVNLINHAIGLLPK"
BASE COUNT         1738 a         1759 c         1888 g         1802 t
ORIGIN      
        1 ggtccattaa agaataattg gcccgcccaa aataggaaag cagacggctg gggatggtaa
       61 cactcgaaga aaattgcgat gtgccattca cctggtcgta ttggttgatc tgggcaaaag
      121 ccgttgtctt gtcaaaattg gaagggaaat ggtttgcctg gatatccatg ctgctgccac
      181 ccgaattgga tacttcctgt cctgccaaaa ggttcagtct gttattgcta ttgatgatga
      241 aatcatagtt aagggtattt gaccaacgca ttccccagct gtcggatttt ccataatcga
      301 cggcgcctgc gaaaaggtgg tttcctgcat cgtcgacata gttgttgtaa acagcgcccg
      361 accaatatcg cttttggccc catgacctgc tcagggtcag atcggtgtga tagacaagtc
      421 ctttgatgat cttccagtcc agcgacgcga tggcccgggc gccctggctg atcgaaaggg
      481 gattgaaatc cgcaatacgt gcagcaggac tatacagatc ccacatcgca ttttttccgt
      541 attgttcgat attgcctgtt ttcaatgcat tggtatcgcc cagaatgtga ttagtcgcga
      601 tcgggcggaa acgataggcg gtagagagta ttgacccgct gccgttggtc gtgccttcat
      661 catccatatc ctgaatatcc gtatagcgcg tgtccagatt gaagatgagg ttgtcgaata
      721 gtttttgact aagcttaaac gatacgttgg cgcgtttcag gtaggaattt attttcatcc
      781 cctgttcgtt cgcataattt acagagaaca gcatttttgt tctgtctgtt ccgcctgtaa
      841 tcgtcaggtc atgatttgag gatgtggaat tgttatacac cagtttttgc tcattgtctg
      901 tttgtaaggt ccggtagctt tcgatcccac cggtattttt gccggcgttg gctcccaggc
      961 caaatagttg ttcaaaaggc gtttgataag cagcgccgtt ggctgcagca ttggcccata
     1021 cgtatttcag atagtcatag ggcgccagtt cacccaggta ttttgcaggg gtattgaatt
     1081 tcacatagcc attgtagctg atcctggttt ttccttcctg tgcgcctttg gtggtgacaa
     1141 ggacgacacc gttggctccg cgggcgccgt atattgccgt agaagaggca tccttcagca
     1201 cgtcgatggt tttcacctga tcaggcggga tatcgctcaa tgtaccaggt accccatcta
     1261 tcaggatcag gggctggttg ctttgcgata tggaaccgcc gcctcttacg cggatctcta
     1321 catctgcatc cggacggccg tcctgggaaa ccacgcttac tcccggcagt ttgccctgca
     1381 tcgcctgggc gatgtttgga acggggatgg cggagatggt ttttccgctg actgaagcga
     1441 cggagccggt cagatctttc ctggtagtcg ttccataacc gatgaccact acctcgtcta
     1501 aattgctggg ggtggattgg aacgtgatat ccagccgttg ctggccgttt agtttgatct
     1561 ccttcgaggt gtagcctaca taggagatca ccagggttgc attcgggccg gaaacgtcaa
     1621 tagtaaagct cccgtcttct cttgtaaggg tgcttgtgga agcgcccttt tcggcaatcg
     1681 ttgcgccggc caggggttca gccgtagact gtttgacttt tccttctacg tgaaattttg
     1741 actgaccgat ggcggttgtt gcgagggaaa gaaagaaaaa taaagcaaag gttagctttc
     1801 gtgtaacaag cataaacata ggtcgtagtt ttggttaaga cgtatatcgc aaagaattca
     1861 caaagggcaa gaaaagtgga ttagacactt tcttttaaca tacaagcaga gtgggaaatg
     1921 aatgaaaatt aaagatagct tcccggggtt aaaaaggggg gattgggctt cgaaatattt
     1981 acgcaatcgt tgccggtgga ttggggaatt gggatttttc ggatcgaggg gcgagggcgg
     2041 gatggggcca tgaaattgtt tatatattat tgattgccaa tgctttgagt tttgtcgggt
     2101 tggtcgcttt ggaggatgct attgataaag atgacgatga gttgcaactc gtgaaaagaa
     2161 tagcctagag cagttctttg gaagcgccat gattcagttg gcaattgcgt gaattcaaac
     2221 gggggccaaa gatccgttca atggaccgtg ttttggaaca agggaagatc ataagtgaca
     2281 taagcattaa tgggattgcc atttataata agaattggca cagggggaag ataaaattgg
     2341 ggaatttttg cccgatttcc ttacgtgagc gagggaatca ccgaataatg cggcggtcca
     2401 aaatgtcatc gaaatcgttg tagagccttc ttacgaaaca tctcctgggg tccggaccga
     2461 ccccatttgg ggtggaacaa gatttgtttt atatattggc gaacgtgtgt tttggacttt
     2521 ttaaacgtgt gttatggaaa tgcctctcaa tagcctgtct gggtcggtcc tgcggaaact
     2581 tgccctcaat gaattaaggc aattcattcg cctgcttgac gatggtacga ctgcagaatt
     2641 gcaaagtaag aaggcgtatc tgacagcaat ttttgcaaaa ctctcggaaa aggaacagga
     2701 agagttccac cgcctgatct acctggtggg aaacgtagat acatcctttg cggatgctgg
     2761 tcccgcgcct cttcagaaga gtgcctaaga catttaacag ctatatcaat tctagggttc
     2821 tttttgagga ctttcattga agggtattag cctgatagtg tcggtataga gttttcctgg
     2881 cctatctcct ttccgaaatt ttcgggattt tcatgccgat gtcggagaag gcgtgctttt
     2941 gcaatattga cgcaagggat ttgagcattt cgtcagatcc gggattgtgg ttggttttgc
     3001 tgataaaacg caacgctcca agggcagccg ttgcagtccc gaatggagaa aatccaggtc
     3061 gtcgggatca tcatccgcca gtaggagata tttataacga cttttcacta aaattcaaga
     3121 ttcatgccga atgggtcagg gcagtgatct ttgtcacttt tcccggtaaa aaatgtcacc
     3181 ggaatctcct tgccgtttga ttagttttgt cgcatgaaaa agtccggaat gggcgtcagg
     3241 ttgacagcgc ggatcctcca ggagtggaaa gatctcggct ttcgttttgt cctggttaag
     3301 ggctatacgg tagaccgata tgccggctgc atcgaattga atcattttat ggtggagccg
     3361 gttaaacaac tccccgagga ccccgccgac caggaaattt atgaacccat tgacagtgaa
     3421 atattgctcg ggtgggctag ttccacagag agcggtgtgc tggcatttat ccaaaaagac
     3481 tcgtaattag atgtgtgctg aaaaccaaaa gaacaaaccg ggctattggc ggagcctgtt
     3541 ccatcataag tgttcgcgct gcaggaccgg cgatatgttc aaggtacgca gctcctataa
     3601 tcttagacag ttcatgaaaa tgtatgacaa gtgccccgtt tgcgatcagg acctggagat
     3661 cgaagtggga ttctattatg gcaccagcta tatcagctat gccatcaccg tagccttgtc
     3721 tgccgttaca tgtctcgcct ggtgggtgtc gatcgggttc tcgctcaacg acggcaggtt
     3781 tttctggtgg atgggatgca atatcgtcgc cctggtgatc gctcaaccct ggttaatgcg
     3841 gttttcccgg gccatttggt tgtctttctt cgtcaggtac aacgagaact ggcaggtaga
     3901 gcggcccgcc gatcctgagc gaaaggggtc caggaaaccg gattgatcgt ggtgtgaaaa
     3961 cacgccctac atcccggatg gccagcaatg caatggagca atatacaaat ggcaagaatg
     4021 aagctaatac agctgaagga tgcgatgcag agccatatat caccccgatg aagaatgaac
     4081 acaacgataa catccatgca gccgtccccc tgacgctgca cacgctgaaa gagttgaaaa
     4141 accagggatt ccggtatgtg ctggtgaatg ctttttcaca tggcagatgc ccggattata
     4201 tcgagcccag ttatttcatg ctgtctcctt taatgcacct gtccgaggac agggaacaaa
     4261 aaggtatata cgaatcgatc gacagccaga tcctggtcga ctgggcaaac actcccaatg
     4321 aagggatcaa agtttttatt acaaaaggat agcagacccc ttttattttc ttttcctgat
     4381 cagatccagc acgccgttga tgaaggtcag gggatggacg ggattgcccg gtacatacag
     4441 atcgatctta taattctgca ggaaaccccg gttcagggat ggcctcccgg caaagatccc
     4501 gccgctgatg gcatctgtcc ccgccaggat gatgatcttt ggttcgggga tggcctcata
     4561 gcagatccga agcggttcgg ccatattctc ggagatgggg ccggtgatga cgatcccgtc
     4621 cgcatgccgt ggggaggcca cgaactcgac cccgaaccgg cccatgtcga aatttacatt
     4681 cccacatgca ttcaattccc attcggtgct gctatcgcca ccggcacaca cctgccgcag
     4741 cttcagcgag ccgctgaaat acttcgatat ttcttcccgg atagcatcgg ggttcagccg
     4801 gatggggaca tctacccctt cccgtacgat cagttcattc cggagattag tggcaatcct
     4861 atagtccctg gtaaaacata tcttctccgg aaaggaaaac gcgcactcgc cgcagaaagt
     4921 acacttcccc aggtggatgc taacaggatc accgctaata gctcccgtgg gacatagctc
     4981 cgccaatgca tggctatcga ccggcgaagt gctgatcacc ggcctccccc ggaaattgcc
     5041 cggtacctgc gctttcgtca catcggggat atattgtttt ccctggtgga ggaggatctt
     5101 gaaattgtcg agcatacgta tcaaaattta aaggtcatga ccgcagtagc ttagattaaa
     5161 gcttttgttg caaatcggaa aatcggaaat ttcattgttt cggacagcca gggcaaccgc
     5221 cagccagtta tgaaaggagg ggtcctttac tttataatgc agcagctcgc cggcgtcatc
     5281 tgtgacggcg cagtggcaga tctcaccccg ccagccttcg acaagagaaa tggcgaatgc
     5341 acccgcggcg ggtcttcccg gagcgtcggt gcgggtagcc ggcaccggaa tattccttaa
     5401 ccattcacgc gcgtaccggg tggattgtgc gatctcctcc tttcgcagct gcgtccggga
     5461 gtagacatct ccatgatgtt tgatgatggg ctgatgcacc gtcctcctga acagaccaaa
     5521 gggatgcgat tcacggatgt cccggtcgat ccccgaagtt cttgccacca tccccacggc
     5581 cccgatcgaa gctgcctgct cacgggttac tactcccgtt ttttccaacc gtgccagcac
     5641 tgtcggaagg cttttcattt catcgaacat ttcggtcaga gcgggttcat aggcgtccag
     5701 caggtggcca agtcgctgcg ccaggctgcc gttaaacggg aaatggctcc cccccacccg
     5761 tatcaaccct ttggccagcc tattcccgca ccattcctgc ataaaattga tgagcggggt
     5821 gcgcagccgc ccgaaaacgg cgctgcccaa ctggtaggcg atgtccgtac agatagcgct
     5881 caggtccccg gtatggatgg ccatccgctc cagctccagc gccagcgtcc gggaaagctg
     5941 cagctcctcc ggcggatcga acccgctcag gctttcccat aggttgacaa aagccaggct
     6001 atgtcccacc acggtatcgc cggcaatgga ttcggcaatg gtgacacgct ccaacggttt
     6061 tttcctgctc acgagcagcg actcgatgcc cctgtgctga tagcccagct ggatatccag
     6121 gtgaaggatc tgctcgccat tgcagataaa tcggaaatgc cccggctcga tgatgcccgc
     6181 atggatcggc ccgacgccca cctcatgcag ctcttcactg tcgatacggt agaatggatg
     6241 gttggctatc gtcttgcttc tgtccgttcg gttgaatgca taccgcaatg gcttcaacca
     6301 ggggtggtcg gtataggaca gaccaaaatt ttccgccagc tcgcgctcga aaccctggaa
     6361 tgccaggttc cttgcagtga acgacggcaa tgcccctcca tgcgtactga tctccgccga
     6421 tgacagatgg atcgaatgct cccggtcatg ggcaatgcag cagatcagct tgatcccgtg
     6481 cttccatcgg aatccataat aattgacgca atgactttct gctcgctcca acatctttat
     6541 gttcatctcc aggaagtcgt catagtccag cacaggaata tcccgcaaag gaatggtttg
     6601 attgttcgat atggaaatac ggttcatagt attattttgg caaaagccct atagcatggt
     6661 tgatgagatt cacaaatacg cccggtggaa agaaaccaag gtaaacgaca cccgccagga
     6721 gcacatactg gctcagggat tctactggcg ggatcctttc taccgccccc tcatcaaatc
     6781 ccaccggctt tataaaaagg atcttgaaaa tattctttcc gaaacaccag atgatgacgg
     6841 tgagcagtag cagcaccgcc gccagcaacg caaaatgtcc cccggcgaaa agggcgctga
     6901 agaggaggaa ttcgctgatg aaaagacccg aaggaggcat ggccgtgatg acgaaaaacc
     6961 cggctaataa aaccattgcc cccgcgggat tgaatcggaa ataatctccc gcatcataaa
     7021 tgctcttgct tttatagatg cggtacacct gtcccacctg gaagaacatc gccgatttcg
     7081 cgaaggaatg caggacgatg tggagaatag cggcataata ccctactccc ccggccgcca
     7141 gacccagcgt gaccagaccg atgtgctcca cgctggaata ggcgaac
//
LOCUS       DASYSV010000064         3761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1936427, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000064 DASYSV010000000
VERSION     DASYSV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936427"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="VGQ51_01955"
     CDS             complement(<1..1530)
                     /locus_tag="VGQ51_01955"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEV9035348.1"
                     /translation="MKAVIGFFGLALVGMIGFGWVRVERQAGPGMRGASLVGSGMPGA
                     RLEGPGMRGARLEGPGYRFPWEQAGLTERQAAAHLVSRLTYGATPGEIDAVVRMGLEK
                     WFQQQLEAQVPDDSLDSVLSRYDALGLSNNEVVETYPRNGQVLRMAVRDGVIDKDSVK
                     TDKKEYREILKNYMEQKGLRRQQELFRQFFCQNILRAAYSNDQLQEVMTDFWFNHFNV
                     SITKNDCAEFIPAYERDVIRPNALGRFNELLLETAKSPAMLYYLDNFSSSAPVDGRDS
                     MRGLRAEGRGRSEGGRDSGRVQAGAGQARRARGINENYAREVMELHTLGVDGGYTQQD
                     VTQAARVLTGWGIYPMGDVAKGAAANMNRFSAEQIARRGFVHDGDFFFNANRHDKGEK
                     VVLGHVFPAGGGYEEGVQLLEMLAHHPSTARFISRKLAVHFVSDDPPQSLIDKMAKTF
                     LSKNGDIRQVLITMVTSPEFWSPDALREKTKSPFELAIGAIRGMHATIYQPMQLYNWV
                     AK"
     gene            1676..2095
                     /locus_tag="VGQ51_01960"
     CDS             1676..2095
                     /locus_tag="VGQ51_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035349.1"
                     /translation="MMRSLLLAGIGLGFCLSIHAQIPDSSLQQSAQFKLPPAQQAARQ
                     VKNLEKKLRLTPDQVLQLQVILINRDVAMDSARNNPSGNHRTEAHTRRGIMQQADRQI
                     DAMLTDDQKALYQQWKQQQKEQAKRRRRLNTSTPPQP"
     gene            complement(2123..>3761)
                     /locus_tag="VGQ51_01965"
     CDS             complement(2123..>3761)
                     /locus_tag="VGQ51_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9035350.1"
                     /translation="AVEKSTAKIEVILHPLDKWRLYSQFQNGKAVGGFIQYVRIFGLV
                     GLLVLVIACINFVNLATARSERRAREVGLRKAIGSARMDLIVQFLIESLAIAFVAFLF
                     SLLLVQMALPGFNRLAQTRIDLPYSSPVFWAVMTGYVLLTGLLAGIRPAFYFSSFQPV
                     KILRGVMQAGTMASLSRKMLVVVQFACSFALITGTLIIYHQIKYGKDRPTGYDPSGLL
                     EVDMTHDLQKNYKPLKNELLQDRLVESISTASNTPDGFSASLAVTGWPGQKAGESLEM
                     PITGASMEYFRTVGMKMKAGREFNSNADSTNIILNEAAVKRLRLVDPLNSLITMEDSS
                     KHLRVIGVVGDAVMLSPFQAAIPSIFTYNPNWANNILLRLPSSANIHESVARLTAVFN
                     KYNPSSPMMLHFADENYAHKFDAETMLGTLAALFAGLAILISCLGLFGLSAYVAEQRN
                     REIGIRKIVGASVWQIWRLLSTNFILLVGIGCLVAIPVAYYLLSGWLDQYDYRISIGP
                     GVFLLATGIAILIAVGTVSFQVVRAARANPVKSLRRE"
BASE COUNT          891 a         1242 c          850 g          778 t
ORIGIN      
        1 cttcgccacc caattgtaga gttgcatggg ctgatagata gtggcatgca tcccccgtat
       61 cgcgccgata gccagctcga acggcgattt cgttttttcg cggagcgcgt cgggcgacca
      121 gaactccgga gacgtgacca tcgtgatcaa tacctggcgt atatccccat tcttcgacaa
      181 aaaggtcttt gccatcttat cgatcagcga ttgcggggga tcgtcactga caaaatgcac
      241 cgcgagcttc cggctgatga accgggctgt cgacggatga tgcgccagca tctccagcaa
      301 ttgcacgcct tcttcatatc ctccgccggc aggaaataca tgtcccaata cgaccttctc
      361 gcctttgtcg tgacgattgg cattaaaaaa gaaatctccg tcatggacaa aaccacggcg
      421 ggcgatctgt tcggcgctga atcgattcat attcgcggcc gcgcctttcg caacatctcc
      481 catcggatag ataccccaac ccgtcaatac cctcgccgcc tgcgtcacat cctgttgcgt
      541 ataccctccg tccaccccca aagtatgaag ctccatgacc tcccgcgcat agttctcatt
      601 gatgccccgc gcgcgccgcg cctgtccggc tcccgcctgc acccgtcccg aatcccgccc
      661 cccttcggac cggccgcgac cctccgcccg gagcccccgc atcgaatccc gtccatccac
      721 gggcgcacta ctgctgaaat tatccaggta atacaacatc gccggcgact tcgccgtctc
      781 cagcaacaat tcattgaatc gccccagcgc attcggccgg atcacatccc gctcataagc
      841 agggatgaac tccgcacaat cattctttgt aatggaaaca ttaaaatggt tgaaccaaaa
      901 atccgtcatc acttcctgca gctgatcgtt cgaataggcg gcccgaagaa tgttctgaca
      961 aaagaactgc cggaacaact cctgctgacg cctcaatcct ttctgctcca tataattctt
     1021 caaaatctcg cgatactctt ttttatccgt ctttaccgag tccttatcga tcactccgtc
     1081 ccggacggcc atccgcaaca cctgcccgtt gcgcggatac gtctcgacga cctcattatt
     1141 acttagcccc agcgcatcat aacggctcag caccgagtcc agcgaatcat ccggcacctg
     1201 cgcctccagc tgctgctgga accatttctc caatcccatt cgcacgaccg catcgatctc
     1261 tcccggtgta gcgccataag taagacggct gaccaggtgc gccgccgcct gccgttccgt
     1321 caatcccgcc tgctcccagg gaaatcggta tcccggtccc tccagtctcg cacccctcat
     1381 acccggtccc tccagtctcg cgcccggcat acccgacccc acgaggctcg cacccctcat
     1441 acccggtccc gcctgccgct ctacccgcac ccagccaaac ccgatcatcc cgacgagcgc
     1501 aagcccgaaa aacccgatca ccgctttcat tgttgtaatt ttttgttaga cgcccccatc
     1561 ccttttttag acgcccccat cccttttttt agatgccccc atcccccaaa ggtttaattc
     1621 catcgggtat taaaccttcc atcccctccc gagtcacata acaaatttac tacaaatgat
     1681 gcgatctctc ctgctggccg gcattggtct tggcttctgt ttatcgatcc acgcccagat
     1741 ccccgactca tccctgcagc aatccgcaca attcaaactc ccacccgccc aacaagccgc
     1801 acggcaggtc aaaaatctcg aaaagaagct ccgcctcacc cccgaccagg tgctccagct
     1861 ccaggtgatc ctcatcaacc gggacgtggc catggacagt gcccgaaaca atccctccgg
     1921 caaccaccgc acggaagccc acacccgccg cgggatcatg caacaggccg accggcagat
     1981 cgatgccatg cttaccgatg accaaaaagc gctttaccag caatggaaac aacaacaaaa
     2041 agaacaggca aaacgacggc ggcggctcaa tacgtccact ccaccccaac cctgatcccc
     2101 caaccgcgcc tccgcccccc actcattcgc gccgcaacga tttcacggga ttggccctcg
     2161 ccgccctcac cacctggaag ctaaccgtcc cgacggcgat gagaatggct atcccggtcg
     2221 ccaacaggaa aacgcccgga ccgatactga tccggtaatc atattggtcc agccagccac
     2281 tgagcagata ataagccacc ggtatcgcaa ccaaacaccc gatccccacg agcaggatga
     2341 agttggtaga caacagcctc catatctgcc acaccgatgc tcccacgatt ttccggatgc
     2401 cgatctccct gttacgctgc tccgccacat aagcggacaa tccgaacagg cccagacaag
     2461 agatgaggat cgccagtccg gcaaacaacg cggccagggt acccaacata gtctccgcgt
     2521 cgaatttgtg cgcataattt tcatcggcaa aatggagcat catcggcgag gaagggttgt
     2581 atttgttgaa cacggctgta agtctcgcga cactctcatg aatattcgcg gatgacggca
     2641 atcgcaacag tatattgttc gcccaattgg gattataggt gaagatactt gggatagccg
     2701 cctggaaagg ggatagcatt acggcgtccc cgacaacgcc gatcacgcgc agatgtttcg
     2761 agctgtcttc catggtgatc aggctattca gcggatcgac cagacgcaac cgtttgaccg
     2821 cggcttcatt caggataata ttcgtactat ccgcattact gttaaattcc ctcccggctt
     2881 tcatcttcat cccgaccgtc cggaagtact ccatggaagc accggtgata ggcatctcga
     2941 gtgattcgcc cgccttctgg cccggccatc ccgttacggc caaggaggca gaaaagccgt
     3001 cgggcgtatt actggctgtc gagatggatt cgaccagtct gtcctgcagc agttcattct
     3061 tcagaggctt atagttcttt tgcagatcgt gggtcatatc cacttccaat aatccactgg
     3121 gatcatagcc tgtcggcctg tccttgccat acttgatctg gtggtagatg atgagggtac
     3181 ccgtgatcaa ggcaaaagaa caggcaaact ggacgaccac cagcatcttc ctggacaaag
     3241 aggccatggt cccggcctgc atcacccccc gcaggatctt caccggctgg aaagaagaaa
     3301 aataaaaggc cggccggata cccgcgagca atcccgtaag cagcacatag ccggtcatga
     3361 ccgcccagaa taccgggctg gaataaggta ggtcaatacg cgtttgagcc aggcgattga
     3421 acccgggtaa ggccatctga actagcaata atgagaatag gaaggcaaca aatgcaatag
     3481 ccagcgattc tatcaggaac tgcacgatca ggtccatgcg cgccgaaccg atggccttgc
     3541 gaagacccac ttcccttgcc cgtcgctcgg accgtgcagt agctaaattt acgaagttga
     3601 tgcacgcgat caccagcacc agcaaaccta cgaggccgaa gatccgaacg tattggataa
     3661 atcctcctac ggctttgccg ttctggaatt gcgaatacag ccgccattta tccagcgggt
     3721 ggaggatcac ttcgatcttt gcagtggatt tttccacggc c
//
LOCUS       DASYSV010000065         7311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1679771, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000065 DASYSV010000000
VERSION     DASYSV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679771"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2686)
                     /locus_tag="VGQ51_01970"
     CDS             complement(<1..2686)
                     /locus_tag="VGQ51_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9035351.1"
                     /translation="MNTSTKRLLWTLLPPLLACSVAMAQQRTITGAVVDETTGEPVAG
                     VTVAVKGHPADATATDSMGIFHLHPTTAGRIVLQFSSVGYESQDFRYGANQASIRIAL
                     KKSDQSMDEVVVIGYGQTVKKRDLTGAISVVKAADIVRAPTNDPLSAIEGMVPGADIT
                     VTNGKENAADSIHIRGTHTFNGLTQPIFVIDGVQGGNLSTLNPNDIESIEVLKDASST
                     AIYGSQGANGVIVVTTKHGHAGKVKVNYNGYMGTNGFVQYPQPRTGQSYVGLRRAAYQ
                     ASGLWNSPADDSKIFSAGEMAAIQNNQWVNWVDIVTNAGLRQSHNVSVSGGNDKSKAY
                     LSAGYYGDDGMIKGNNQKQYNLLLNLDQTIAPWIKTGVQAGLVYSNINTRSSDPYSLA
                     ETAVPLGTAFDSAGRINVYPIAGAASTMSPLSDDRGPLIATNNNVSTATSINVHLDIT
                     PLKGLSVVSRFGANFFSSRSGQYFDSSSLEEINHKYSYGAAINNSTHFYEWDNIVTYN
                     KDFGPHSFTLTGLSTYTQNVKETYSVSGTGLIYSSQLFYSLQGAPDNRFISSGYVQQN
                     NMSYAGRLHYAYKGKYIFDVTDRYDGASILAVGHKNYNFPSAAAAWRVSDEGFMQNLN
                     AISNLKLRFSYGVAGNSGIPAYGTQSFLVVQNMGFENTNAPAYIFNTSIGNTNVGWEL
                     SKTANLGVDLGLFRNRIDLSVDVYNTNTDNILMLRSLPPSLGVGNTWQNVGATNNKGI
                     EVALNTRNIETPNFRWMSTVSFMSNRERVTSLINGTNIIDGQNAEVNSLLLGHPVHSF
                     YNYVKQGIWQTDEASKAANLVFGTTPFAPGSIKLKDLNGDNKISPDSDRTYIGSQVPK
                     FSLGFQNTFVYKNFDLTVYAILRYGQMIQAQFLGRYYPN"
     gene            complement(2702..3769)
                     /locus_tag="VGQ51_01975"
     CDS             complement(2702..3769)
                     /locus_tag="VGQ51_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9035352.1"
                     /translation="MTIETNSPLPQHPRPVILIGAGGIVNDAHLPAYKIAGFWVAGIY
                     DVDREKALRTAAKFDIPLVYYSMEDMLDRSPRSVVFDIAVPGSQMLPVLRQLPEGACV
                     LLQKPMGDDLSMAREILGLSRQRRMTAAVNFQLRYAPFINAARNIVKQGLIGELVDIE
                     FNVNVYTPWHLWKFLYSLPRVEILYHSIHYIDLVRSFLGNPESIYAKTVKHPAMAELA
                     SVRTNIIMDYGPTIRANILTNHCHEFGLQQQQSYLKFEGTRGAIKIKMGLLMNYPEGV
                     PDSFEYFLKHDETPGEWKTLDVPGGWFPHAFIGSMAQVMLAAEGSIERPDNSVEDAIH
                     TMACVEAAYESSERGGVIPNF"
     gene            complement(3775..4827)
                     /locus_tag="VGQ51_01980"
     CDS             complement(3775..4827)
                     /locus_tag="VGQ51_01980"
                     /inference="COORDINATES: protein motif:HMM:NF018123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEV9035353.1"
                     /translation="MNVLIGLTMLILGGISAGSSYVPIKGIRGWAWESLWAVQGVVAW
                     LLLPMLLALLATPHLFVLLSRIPLSTLTSTFLFGLLWGLGGLAWGMAVRYIGLALGFT
                     IATGFLSSLGTLVPIIAGGRMGQIWSTGSGRITLLSIALSLVGIVICGRAGWKRERDT
                     AGQSAGGVAGAAGETFQFKKGILIAFAAGVLGACFAFGIAAGEPIRQQAIADGAAPVW
                     AASPVFFVELLGGITVNLFYCLWMNRRNRSWRDYRAAGKTGDLKTNYGLSALVGVLWY
                     LQFVFYGMAAFFMGPYSFANWSVHMSFMVVASNLWGYYYKEWKGASRATIRTNTAGIL
                     LLVISGIIMGVAGYSK"
     gene            complement(4824..5684)
                     /locus_tag="VGQ51_01985"
     CDS             complement(4824..5684)
                     /locus_tag="VGQ51_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9035354.1"
                     /translation="MNVIDTHIHVWDLDRAEYPWLKGDTSILNRTYEIEELEEERMRT
                     GVTHGVLVQAAYNLEDTDWMIRVAETHPWIAGVVGWLPLEDPSATAALLERDYVTGGL
                     LKGVRHLIHDEADTEWLLQDTVLESLGLLAARDISYDIVGILPAHIATALKVAARRPG
                     LRMIFDHVNQPPPPADGPLEDSVWATLMKEAAANPNFYVKISGLATAARKGRQWKADD
                     LEPGIAFAARHFGVERICCGGDWPVSLLAGGYADTWNAYRTIIARHLNEDDQAKLLSQ
                     NAERFYKLAI"
     gene            complement(5781..6932)
                     /locus_tag="VGQ51_01990"
     CDS             complement(5781..6932)
                     /locus_tag="VGQ51_01990"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV9035355.1"
                     /translation="MNLLKDILVVDFSQFLSGPSAGLRLADMGARVIKVERPGTGDIC
                     RQLYVSDTRIEGESTIFHAINRNKQSYAADLKKGEDLEKIKKLIQKADVVLHNFRPGV
                     MERIGLDYSTVRSIKPDIIYGEVSGYGNEGPWKDLPGQDLLVQALSGLTWLSNDRNAR
                     PTPMGVSVVDILAGTYLTQGILAALYRRGVNGSGGLVQVSMMEAVLDFQFEVLTCHYN
                     DGRQLPQRSQVSNGNAYIPAPYGIYKTREGYLSLAMGDIPILARLLECPPLENYTDKA
                     EWFDRRDEIKAILTRHLLNRTADEWLSILQPADIWCARVMDYHELMQQLGYKVLNMEL
                     KVKTSNGLSIRTTRCPIRVDGQMAASEKGAPLLGEHNALIDEQFGLGAE"
     gene            complement(6942..>7311)
                     /locus_tag="VGQ51_01995"
     CDS             complement(6942..>7311)
                     /locus_tag="VGQ51_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV9035356.1"
                     /translation="PDLAKAISSEELAAYRQEQAFSHRDEIKEVLTRELAKHPTAYWL
                     ERLHAEDMWAMEVLDWKRLAAHPGYQVLEMEQPIDTGEGRTMITTRCPIRIDGRRLYS
                     PRPAPSLGQNNTEIGMELLK"
BASE COUNT         1602 a         2068 c         1976 g         1665 t
ORIGIN      
        1 cattggggta ataccggccc aggaactgcg cctggatcat ctggccataa cggagaatgg
       61 catagaccgt cagatcgaag ttcttataga caaaggtatt ctggaagcca agcgagaatt
      121 tcggtacctg cgagccgata tacgtgcggt cgctgtccgg agagatctta ttgtcaccgt
      181 tgaggtcctt cagcttgatg gaacccggag cgaagggcgt ggtgccaaaa acgaggttgg
      241 cggccttact ggcttcatcc gtttgccaga tgccttgttt cacgtagttg tagaaggagt
      301 ggacgggatg accgagcaac agcgagttga cctcggcatt ctgcccgtcg atgatattcg
      361 tcccgttgat caggctggtg acccgctcgc ggttcgacat gaagctgaca gtgctcatcc
      421 acctgaagtt cggcgtttca atattccggg tattcagagc gacctcgata cctttgttat
      481 tcgtggcgcc gacattctgc caggtattgc ccacgccgag gctgggaggc agcgagcgga
      541 gcatcaggat attgtcggta ttggtattat atacatccac cgagagatcg atccggttgc
      601 ggaacagacc gaggtccaca ccgagattag ccgtcttcga caattcccag cctacattcg
      661 tatttccgat gctggtgttg aagatatacg cgggggcatt ggtattttcg aagcccatgt
      721 tctgcacgac gaggaaggat tgggtgccat aagccgggat acccgagttt ccggctactc
      781 catagctgaa ccgaagtttc agattggaga tggcgttgag gttctgcatg aagccctcgt
      841 cgctgacccg ccaggccgcg gcggccgacg ggaaattgta gttcttatgt cctacggcca
      901 ggatagacgc gccgtcatac cggtcggtga cgtcgaatat atatttgcct ttatatgcat
      961 agtggagcct accggcataa gacatattgt tttgctgcac ataaccggaa gagatgaacc
     1021 ggttgtccgg tgcgccctgc aggctgtaaa acagctggct gctatagatg agacccgtac
     1081 cgctgaccga gtaggtctcc ttgacgttct gcgtataagt cgagagtccc gtcagtgtaa
     1141 aactatgcgg tccgaagtcc ttattgtaag tgacgatatt gtcccattcg taaaagtgcg
     1201 tcgaattatt gatcgcggcg ccataggaat atttgtgatt gatctcctcc agcgaagacg
     1261 agtcgaaata ctgaccgctg cggctgctga agaagttggc accaaaacgc gagacgacgc
     1321 tcaacccctt caaaggcgta atatccagat gcacgttgat cgaagtagcc gtcgagacat
     1381 tgttattggt agcgatgagg gggccgcggt catccgacaa cgggctcatc gtgctggctg
     1441 cgccggcaat cgggtaaacg ttgatacgtc ctgcgctatc gaaagccgta ccgagcggta
     1501 ctgccgtctc tgccagcgaa taggggtcgg acgagcgggt attgatattg gaatacacca
     1561 ggccggcctg tacacccgtc ttgatccacg gcgcaatcgt ctgatcgaga ttcagcaaca
     1621 gattgtactg tttctgatta ttacctttga tcatgccgtc atcaccatag taaccggcgg
     1681 acagatacgc tttgctttta tcgttgccgc cgctgacgga cacattatgg ctttgccgca
     1741 gaccggcgtt ggttacgata tccacccagt tgacccattg gttgttctgg atggccgcca
     1801 tttccccggc ggagaatatc ttcgaatcgt cggcagggct gttccatagg cccgatgcct
     1861 ggtaggccgc gcgacggaga ccgacatacg actggcccgt acgcggctga ggatattgca
     1921 cgaagccgtt ggtacccata taaccattat agttgacttt gaccttgccg gcatgaccgt
     1981 gctttgtggt cacgacgatc actccattgg cgccctgcga cccatagatc gcggtggagg
     2041 atgcatcctt cagaacttcg atggactcga tatcgttcgg attcagcgtg ctgagatttc
     2101 ctccctgtac gccgtcgatg acaaaaatgg gctgagtaag cccgttgaag gtatgcgttc
     2161 cgcggatatg gatgctgtcg gcagcattct ccttgccgtt ggtgactgta atatccgcgc
     2221 cgggtaccat cccttcgatg gccgacaacg gatcgttcgt aggcgcgcga acgatatcag
     2281 ccgccttgac taccgagatg gcgccggtga gatcccgctt tttgaccgtc tgcccataac
     2341 cgatgacgac cacttcatcc atgctttggt cgctcttctt caaagcgatg cggatggatg
     2401 cctggttggc gccgtaccgg aagtcctgcg attcatagcc gaccgatgag aattgcaaca
     2461 caatacggcc cgcggtcgtc ggatggagat gaaatatgcc catgctatcg gtagcggtgg
     2521 catcggcggg gtggcccttt actgcgacgg tgacgccggc taccggctca ccggtggttt
     2581 catcgactac ggcgcccgtg atcgtccttt gctgcgccat agctaccgag caggccagca
     2641 gggggggaag caaggtccat aagagtcgtt ttgtactagt gttcatatag caattgtggt
     2701 tttaaaaatt tggaatgaca ccgccccgct cgctggattc ataggctgcc tccacgcacg
     2761 ccatggtatg aatagcgtct tcgacggagt tgtcgggtct ttctatcgaa ccttcggccg
     2821 caagcatcac ctgtgccatc gatccgatga aggcatgcgg gaaccagcca ccggggacgt
     2881 ccagtgtttt ccactcaccc ggtgtttcgt catgcttcag gaagtattcg aagctgtcgg
     2941 gtacgccttc cggataattc atcaacagac ccatctttat tttgatggcg ccacgcgtgc
     3001 cttcgaattt gagataggat tgctgttgct gtagtccgaa ctcatgacaa tggttggtca
     3061 ggatattggc gcggatcgtg gggccgtagt ccatgatgat attggtgcgg acggaagcaa
     3121 gctcggccat cgcgggatgt tttaccgttt tggcataaat gctttcgggg tttccgagaa
     3181 agctgcggac aaggtctata tagtggatgc tgtgataaag gatctccacc cggggcagcg
     3241 aatacaggaa cttccagaga tgccagggcg tatatacatt gacattgaat tctatatcta
     3301 ccaattcgcc gatgagcccc tgtttgacga tattccgcgc agcattgatg aagggggcat
     3361 atcgcagctg gaaattcacg gctgccgtca ttcgccgctg gcgtgacagc ccgaggatct
     3421 cacgggccat ggaaagatcg tcgcccatcg gcttttgcaa caacacgcat gctccttcag
     3481 gtagttgtcg caatacgggg agcatctgcg aacccggtac cgcgatatcg aagaccaccg
     3541 accgcggact ccgatccaac atatcctcca tgctataata cacgagaggg atatcgaact
     3601 tcgcggctgt ccgcagcgct ttctccctat cgacgtcata gatgcccgcc acccagaaac
     3661 ccgcgatctt atacgcagga agatgcgcgt cattgacgat gcccccggct ccgatgagga
     3721 tgacgggtcg ggggtgctgg ggtaaagggc tgtttgtttc gattgtcatt tgtcttattt
     3781 tgaataaccg gcaacgccca tgatgatgcc ggagatcaca agcagaagga tgcccgcagt
     3841 attcgtacgt atcgtggccc gcgaagctcc cttccattct ttataataat agccccacaa
     3901 attgctggcc acgaccatga aggacatgtg tacggaccag ttggcgaagc tgtaaggccc
     3961 catgaaaaat gcggccatcc cataaaagac gaattggaga taccacagca ctccgaccaa
     4021 tgccgagaga ccatagttgg tttttagatc gccggtcttc cccgccgcac gatagtcgcg
     4081 ccagctgcga ttgcgccggt tcatccagag gcaatagaac agattgacgg tgataccccc
     4141 cagcagctcg acgaagaaga cgggtgaagc cgcccacacc ggcgccgcgc catcggcaat
     4201 ggcctgttga cggatgggtt cgccggccgc tatgccaaag gcaaaacaag cgcccagtac
     4261 gccggcggca aatgcgataa ggatgccttt tttgaactga aaggtttcgc ccgccgcgcc
     4321 cgcgacgccc ccggccgact gccctgctgt atcccgctct cttttccatc cggcccggcc
     4381 gcagatgacg atacccacca gtgacagggc gatactgagc aaggtgatcc ggcctgaacc
     4441 ggtcgaccat atctgaccca tcctgccccc ggcgatgata ggaaccaggg tccccagcga
     4501 agaaagaaaa ccggtagcga tcgtaaaacc caaagcaaga ccgatatatc ggaccgccat
     4561 cccccaggca agcccgccca atccccacag aagaccgaaa aggaaggtgg aggtcagcgt
     4621 ggacagcggt atccgggaaa gcaggacgaa tagatgagga gtagcgagca gcgccaatag
     4681 catcgggaga agcaaccagg cgacgactcc ctgaaccgcc cataggctct cccaggccca
     4741 gcccctgata ccttttatag gcacatagct gctgccggcg gagatgccgc caaggatgag
     4801 catggtcaaa ccaatgagta cattcatatg gcgagtttat aaaaccgttc ggcgttttgc
     4861 gacaacagct ttgcctggtc gtcctcattc aaatgccggg cgatgatggt gcggtaagca
     4921 ttccaggtgt cggcatatcc gcctgcgagt aaagagaccg gccagtcgcc gccgcaacaa
     4981 attctttcga ccccgaagtg acgcgccgca aaggcaatac cgggctcgag gtcatcggcc
     5041 ttccattgcc gtcctttcct cgctgccgtc gccaaacccg aaatcttgac gtagaaattc
     5101 ggattagccg cggcctcctt catcaacgtc gcccagacac tgtcttccaa cggaccatcg
     5161 gccggaggcg gaggctggtt gacatgatcg aagatcatcc gcagaccggg ccttctcgcc
     5221 gccactttca atgcagtcgc gatatgcgca ggcagaatgc cgacgatatc ataggatata
     5281 tcacgtgctg ccagcaaacc caggctctcg agcaccgtgt cctgcaacag ccactccgta
     5341 tcggcttcgt catggattag atgccggacg cctttcagca agccgccggt cacatagtca
     5401 cgttccagca gcgcggccgt cgcggaaggg tcctccaaag gcagccagcc tacgactccg
     5461 gcgatccagg gatgcgtttc ggccacccgg atcatccagt cggtatcttc aaggttatag
     5521 gccgcctgta cgagaactcc gtgagtaacg cccgtccgca tgcgttcttc ctccagctct
     5581 tctatttcat aagttcggtt aaggatcgac gtgtctccct tcaaccaggg gtattcggcc
     5641 cgatcgagat cccagacatg gatatgtgtg tctatgacat tcattccgcc catttaaggc
     5701 aaattacttc atgccgccgc caaacccatc ccgtgccatc ctgaacccat gcctgtctcg
     5761 atccgaactg cgcccatgcc ctactccgcc cccaacccga actgctcgtc gatcagcgca
     5821 ttatgctctc ccagcaacgg cgcgcctttt tccgacgcag ccatctggcc gtcgacgcgt
     5881 atcggacaac gtgtagtacg gatggaaagt ccattgctcg tcttcacctt cagctccata
     5941 ttcagtactt tatatcctaa ctgctgcatc agctcgtgat aatccatgac cctggcgcac
     6001 cagatgtcgg cgggttgaag gatggacagc cattcgtcgg cggtccggtt cagcaggtgt
     6061 ctggtcagaa ttgcttttat ctcatcgcga cggtcgaacc attcggcctt gtcggtatag
     6121 ttttccagcg ggggacattc cagcagtcgc gctaatatcg ggatatctcc cattgccagc
     6181 gataagtagc cttcacgggt cttataaatg ccataaggag ccgggatata agcattgcca
     6241 ttgctgacct ggctccgctg cggaagctgt cgcccgtcat tatagtgaca ggtaagcact
     6301 tcgaactgaa agtcgagtac agcctccatc atgctcacct ggacgagtcc gccggaacca
     6361 ttcactcccc gtcgatataa ggccgccagg ataccctgag tcagataggt gcccgccagg
     6421 atatccacca cggatacgcc catgggcgtg ggtcgtgcgt tgcggtcatt gcttagccac
     6481 gtgagccccg acagggcctg tacgagcagg tcctgacccg gaaggtcttt ccatggtccc
     6541 tcatttccat acccgctgac ctctccatat atgatgtccg gcttgatgga cctcaccgta
     6601 gagtaatcga gcccgatacg ctccatcact ccgggacgga agttgtgcag gacgacatcg
     6661 gccttctgta tcagtttttt tatcttttcc aggtcttcgc cttttttaag atcggccgca
     6721 tacgattgct tattgcggtt gatcgcatgg aagatggtgc tttcgccctc gatacgggta
     6781 tcggaaacat acagctgtct gcagatgtcg ccggtgcccg gtctttccac ctttatcacc
     6841 ctcgcgccca tatcggccaa ccgcaaaccc gccgagggac ccgacaggaa ctgactgaaa
     6901 tcgacgacga ggatatcctt caatagattc atggacattg gttattttaa taattccatc
     6961 ccgatctccg tattattttg tcccagcgaa ggcgccggtc tcggggagta cagcctccgc
     7021 ccatcgatac ggatcgggca acgggtggtg atcatggtcc tcccttcccc ggtgtctatc
     7081 ggctgttcca tctccagcac ctggtaaccg gggtgtgcgg ccagcctctt ccagtccagt
     7141 acttccatcg cccacatatc ttcggcatgc aaacgttcca gccagtatgc cgtcggatgc
     7201 tttgccagtt cgcgggtcag cacttctttt atttcgtcac gatgactgaa ggcctgttcc
     7261 tgacggtaag ccgcaagctc ttcggaggag atagccttcg ccagatcggg g
//
LOCUS       DASYSV010000066         6108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1936719, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000066 DASYSV010000000
VERSION     DASYSV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936719"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VGQ51_02000"
     CDS             complement(<1..455)
                     /locus_tag="VGQ51_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035357.1"
                     /translation="MLRNILLVTYRNLLKHRSFTLINILGLALGMAAFLLIGGYVRFE
                     KSYDRSYADADHIYRVESQFFKGNVLTNDWPTSTNGYARAIKENIPGIAAITRINWHN
                     SERVVKYNNLKFREEHVCFADSNFLTFFSYPLIKGDAATALSRTHSIVIS"
     gene            complement(484..1035)
                     /locus_tag="VGQ51_02005"
     CDS             complement(484..1035)
                     /locus_tag="VGQ51_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV9035358.1"
                     /translation="MRKLIYAINLAIDGCCDHTKMTGGEDILDYFADLILEADLLIYG
                     RITYQLMVPYWPDVAKENSSSKAEIEFARAFDSVGKVVFSKSLDKVEDKNSRLVRTKP
                     EDEILKLKQQEGKNMLLGGVAFPSHLIELGLVDEYIFVVQPTLVGGGRRLLEGINLPE
                     RLKLKLVGTKILRSGCVVLHYIN"
     gene            complement(1104..3596)
                     /locus_tag="VGQ51_02010"
     CDS             complement(1104..3596)
                     /locus_tag="VGQ51_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9035359.1"
                     /translation="MKKLIMAAGLILALASDSFAQTTQTSGNWSDPSIWSGGAVPAAS
                     GTVKVNNPVTIDGNLSPTGAWTFNSNATDQPGGTAYTFNPGAGTNTITVSPGSIVTFE
                     GGSSGTPNVCSSGTINIYGTLVLGFTQLNNSGNLKITIESGGTLIINGDLTNKNNSGT
                     FTVNGSLIVNGNFANTSGSVTVGGSGTINTTGTITTSGGSTVFGSGNDCSTGPCSGTT
                     LSCTFTNTITPTSKVVCSGTSGIVLTSGNSASSPTYQWLSSTDGVTYANASGTSTNST
                     YTTPTLTQTTWYKVTVKGTGPSCTSTSAVVKLTVVNGGGWLGATSNDWGTASNWCSLV
                     VPTSTTDVVITNATGVTNMPTINAGTAASARSVTISDTYPVSSLTIAASGTASLSIYG
                     DLTLNGSFTDYSTASTAGVVLAGSVAQTISGTTAAVFKNLTINNSSGSASAITLSSNN
                     LSVSSNLTLSSGIVNLSGNTVTLGSSATNTGTLNYTSGSWLYGGNIVRWFPTSAVTLG
                     SNGSLFPIGDASNYRPMYFGSSGLSASGGTIKVSHTGIVNATPVSFLDAATAVLVRSN
                     SYWKVSTGNGISSTGTPFNLRTEGTGFGIIGDVADLRMILAATSAPGTDGVNAGTVGN
                     PQVNRTASSVANLANTYYWGSVNPGYTTLRVSLVSFTAGPRSGEIVLNWRTATEGDCR
                     YFSLERSEDGTNFSEIVSVAAEGGADLGHVYGYTDRSPGGGKNFYRLKMVGSGGAVSY
                     SWIVTADLSAGGGLVLFPDPTDGSAISVRVPVGGNYTVDVYDGQGMVVRHVSCSSPVE
                     RVLFSPSLPPGVYVARVLAQGYSAVETFLVRR"
     gene            4041..>6108
                     /locus_tag="VGQ51_02015"
     CDS             4041..>6108
                     /locus_tag="VGQ51_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035360.1"
                     /translation="MTNYLRSAWRHFSREKGITAITITGLALGLAACIFIALYVTDEF
                     AYDRYNKNADRIFRIASDLHINGGTINDMSTAPAMAAALVRDFPAIENAVRIRDVRRD
                     VEVHVGTRLFSQTGVVLADSTLFKIFTLPMIEGDPGTALDAPNSIVISATTAKKYFNS
                     TDVVGRTLQLDQDTAVCKVTGVIRDMPAASHFHFQLIRSLRQTRQEWINFFATTYVLA
                     RPGIQTADIDRMLATEVERYVYRQVREQMHNSKADLKRNGDYFRYYSMPLTRIHLYSN
                     LGHEWEPNGNIRYVILFIVIAVLILVVAGINFVNLSIARSLRRLRDIGVRKILGSDRR
                     RLIVQFLTESILMTTIAMGLAIFLVLLGLPLFRRLANKSFTAAVFLSHWTVPALVLAT
                     ILVGLLAGAYPALILSRVEPLKILRGQLTLDSSPKTLRTILLVFQFSVAMTLIIGTGV
                     IYSQLSYIQHRDIGYLRQQVVTIKDTYRLGDKAWTFADESRKLPGVIGATVSGDLPNQ
                     KAIYTGFFKDRSASVTSTALLGVWHIDPDYLPTLGMKLVAGRNFSPQLHTDSACALVN
                     ETAARMLGYAHPLGEKVYRGPDSTSAHTIIGVVKDFNTGTLRNPIDPVIFVLAPDGVA
                     VTFRLAPGDIASTMNAIRRRYETVAGGYPFIYSFLDEDFNRQYAADQRTAGLFTVFSG
                     LALIIAA"
BASE COUNT         1419 a         1709 c         1533 g         1447 t
ORIGIN      
        1 gaaatgacga tcgagtgggt tcgacttaat gcggtagcag cgtctccttt tatcagaggg
       61 tatgaaaaaa aagtcaaaaa attactgtcg gcaaagcaaa catgttcttc ccggaatttt
      121 aaattgttgt atttcaccac ccgttcagag ttgtgccaat tgattcgggt gatggcggcg
      181 atgccgggta tgttctcttt tatcgccctt gcatagccgt tcgtgctcgt gggccaatcg
      241 ttcgtaagga cattgccttt gaaaaattgg ctttctaccc ggtagatatg gtctgcgtct
      301 gcatacgacc ggtcatagct cttttcaaac cggacatagc cgccgatcaa caggaatgcc
      361 gccataccta aagcgagccc gaggatattg atcagggtga aggatctgtg ttttagaaga
      421 tttcgatagg tgaccagtaa aatgttccgg agcatgggag aaggaatgaa gattaatcgg
      481 gggttaattg atataatgaa gcacgacgca gcctgatctt aagatcttcg tgcccaccag
      541 ttttaatttt aatctctcgg gcaggttaat accttccagc aaccgccttc cgcctcccac
      601 aagtgtaggc tgaacgacaa aaatatattc gtccaccaga ccaagttcta taagatggga
      661 aggaaacgct acgccaccga gtaacatatt cttgccttcc tgctgtttca atttaagtat
      721 ttcgtcttcg ggctttgtac gaacgagcct cgagttctta tcctcaacct tgtctaatga
      781 tttagaaaaa acgactttgc caacggagtc gaatgcccgg gcaaattcaa tctcggcttt
      841 tgaagaggaa ttctcttttg cgacatcggg ccaataggga accatcaact ggtaagtgat
      901 acgtccgtag atcaataggt cggcttctag tataaggtct gcaaaataat caagtatgtc
      961 ttcgccgcct gtcattttgg tatggtcgca acacccatcg atggcaaggt tgatggcgta
     1021 tatcagcttt ctcatttgat tgcgtttaat cttaattttc tgtcccaaaa atagaatatc
     1081 gcattcaaac tgaaaaggtg cgtttaccgc ctcaccaaaa aagtctccac cgcactatac
     1141 ccctgcgcca aaacccttgc aacatatact ccaggcggca gcgacgggct aaacaatacc
     1201 cgctccaccg gtgatgaaca agatacatgc cgcaccacca tcccctgacc gtcatataca
     1261 tccacggtat aatttccccc gacaggaaca cggaccgaaa tcgcactccc atccgtcgga
     1321 tccggaaaaa gcaccaaccc cccgcccgcg gaaagatcag cggtgacaat ccacgagtaa
     1381 gacacggccc cacccgaacc caccattttc aaacgataaa aattcttccc tccccccgga
     1441 ctcctatcgg tataaccgta aacatgcccc agatccgccc caccttccgc tgcaaccgaa
     1501 acgatctccg aaaaattcgt cccatcctcc gaccgctcca gagaaaaata cctgcaatcg
     1561 ccttccgtcg ccgtcctcca attcagaacg atctcgccgg acctgggtcc cgccgtgaag
     1621 gataccagcg agacacgaag cgtggtatat cccgggttga cggagcccca gtagtaggtg
     1681 ttcgccaggt tcgcgacgga tgaagcggta cggttcacct ggggatttcc aaccgtcccg
     1741 gcattgacgc cgtctgtccc cggtgcggag gtcgcagcca ggatcatacg cagatcggcc
     1801 acatctccga tgataccgaa accggtgcct tccgtacgca ggttgaaagg ggtgcccgtg
     1861 ctgctgatcc cattgcccgt actcaccttc caataagaat tcgatcgtac caggaccgct
     1921 gtggcagcat ccaggaagga gacaggcgtg gcgttcacaa taccggtatg actaactttg
     1981 atggttcctc ccgatgcgct aaggcccgaa ctgccaaaat acattggccg gtaattggaa
     2041 gcgtcgccga ttgggaaaag actgccgttg gaaccaagcg tcaccgcgga agtcgggaac
     2101 caccggacaa tatttccgcc gtataaccag gacccggaag tgtaattcag cgtgccggta
     2161 ttggtggcgc tactgccgag agtcaccgtg ttaccgctga gattgacgat acccgaggat
     2221 agggttaggt tactgctgac cgatagattg ttcgagctca gtgtgatcgc ggaagcggat
     2281 cccgagctgt tattgatcgt caggtttttg aaaactgccg cggtggttcc gctgatcgtc
     2341 tgcgcaacgc tgcccgccag gactaccccc gccgtggaag cggtcgaata gtcggtgaag
     2401 ctgccattga gcgtcaggtc cccatatatg ctcagcgatg ccgtaccgct tgcagcgatg
     2461 gtcagcgacg agactggata agtatcgctg atggtaacgc ttcgggccga agccgccgtc
     2521 cccgcattga tcgttggcat attagtgaca cccgtggcat tggtaatgac gacatccgtc
     2581 gtgctggtcg gcaccacaag actacaccag ttgctggcgg ttccccaatc attgctcgtg
     2641 gctcccaacc agccgccacc attgacgaca gtgagcttta caactgccga ggtgcttgtg
     2701 caggaaggtc cggtaccttt gaccgtcact ttataccagg tggtttgcgt caacgtgggc
     2761 gtggtatagg ttgaattagt gctggttccc gaagcattgg cataggtgac gccgtcggtg
     2821 ctgctcaacc attgataagt gggagagctg gctgaattgc cggaggtaag cacgattccc
     2881 gacgttccgc tgcagacgac cttgctcgtg ggtgtgatgg tattggtaaa ggtacaacta
     2941 agcgtagtcc ccgagcaggg gcccgtgcta cagtcattac cgctgccaaa aacggtggag
     3001 ccaccggaag tggtgatcgt gccggtggta ttgatcgtgc ctgaaccgcc gacagtgaca
     3061 cttcccgatg tgttggcgaa atttccattg acgatcagcg acccgttgac ggtaaaggtc
     3121 cccgaattat tcttattggt caggtccccg ttgatgatca gggtgccccc cgactcgatc
     3181 gtgatcttca ggtttccgct gttgttcagc tgagtaaaac ccaggaccag ggtgccataa
     3241 atattgatcg ttccgctgct gcacacattg ggagtgcctg agctgcctcc ttcgaaggtg
     3301 acgatactgc ccgggctcac cgtgatggtg ttggttccgg ctcccgggtt gaacgtatag
     3361 gccgtgcccc ccggttggtc ggtggcgtta ctattgaatg tccacgctcc tgttggcgac
     3421 aggttgccat cgatggtgac agggttattc acttttacag taccgctcgc cgccggcact
     3481 gccccaccgg accaaatgga aggatcactc cagttcccgc tcgtttgggt ggtttgagca
     3541 aaagaatcgc ttgccagggc caagatcagc cctgctgcca tgatcagctt tttcatacga
     3601 tatgtttgga tgtagctttc ccgccccgac gaattataaa ggctaggggt tttccttgat
     3661 tatctgccag cctttaacta aaagaaacca cttattagta tataagtgga ataaattaga
     3721 ccccggggta gggggacccc ggggaggggg atatatgacc ctggagggtt acttagccgg
     3781 tgaaagaaac gctttcccgc tggcatgatt ttttggttcc tcacctaaaa aaaagactta
     3841 tgatccgaaa gctaaagtcc ggtcagtacc gtatttactc ccggaaaaaa gacgcaaaaa
     3901 ccaacaaaag gcgaaacctc ggtactttcg actccctcga ggcggcaaga aaacacgaac
     3961 gcgagatcca atactttaaa cacggttaat tctcggaata atttttatgg aatccccgcc
     4021 cttttgcatc ccgccaggcg atgacgaact atctgcgctc cgcctggcgc catttttccc
     4081 gtgaaaaagg aataacggca atcaccatta caggtctggc ccttggtctt gccgcctgca
     4141 tcttcatcgc cctttacgtc acggacgagt tcgcatatga tcgttacaac aaaaatgccg
     4201 accgcatctt ccgaatagcc tccgatctcc acatcaacgg cggcactatc aatgacatgt
     4261 cgacagcgcc tgccatggcc gccgcactgg tccgcgattt cccagcgatc gaaaatgccg
     4321 tccgcatccg ggatgtacgc agggatgttg aagttcatgt cggaacccgt ctgttctccc
     4381 aaaccggcgt cgtattggcg gattctaccc tttttaagat atttaccctt ccgatgatcg
     4441 aaggcgatcc cggaactgcc ctcgacgcac ccaactccat cgtaatctcg gcaaccaccg
     4501 cgaaaaaata tttcaacagc acggacgtag tagggcggac gctgcaactc gatcaggata
     4561 cggccgtctg taaggtgacg ggagtcatcc gggatatgcc cgctgcatcg catttccatt
     4621 ttcagctgat ccgatcgctc agacaaacga gacaggaatg gatcaatttt tttgctacga
     4681 cctatgtgtt ggcccgcccc ggcattcaaa cagccgacat cgaccggatg ctggccactg
     4741 aggtcgaaag atatgtctat cgacaggtgc gcgagcaaat gcacaatagc aaagccgatc
     4801 tgaaacgcaa cggcgattat ttccggtatt attccatgcc gctcacccgg atccatctct
     4861 attccaatct gggtcatgaa tgggaaccca acggaaatat ccggtacgtc atccttttta
     4921 tcgtcattgc cgtcctgatc ctggtcgtcg cgggtatcaa tttcgtcaac ctttctatcg
     4981 cccgcagcct gcgccggctt cgtgacatcg gcgtgagaaa gatcctgggg tccgaccgcc
     5041 ggagactgat cgttcagttc ctgaccgaat ccattctcat gaccacgatc gcaatgggcc
     5101 tggccatctt cctggtgctg ctcggcctcc cacttttccg ccgtctggcg aacaagtcgt
     5161 tcacagccgc cgtcttcctc tcgcattgga cggttcctgc tctcgtactt gcaacaatcc
     5221 tggtaggcct cctggccggt gcctatcctg cattgatcct gtcgcgggtc gagcccctga
     5281 agatcctgcg tggacagttg acactggatt cgagcccaaa gacccttcgg accatcctgc
     5341 tggtcttcca attcagcgtg gccatgacgc tcatcatcgg tacaggcgta atctattcgc
     5401 agctatccta catccaacac cgtgacatcg gatacctccg tcagcaggta gtgacgatca
     5461 aagacactta caggctcggc gacaaggcct ggacctttgc agatgaatcc cgaaagctac
     5521 ccggggtcat cggcgctact gtatccggcg acctgcccaa ccaaaaagct atctacacag
     5581 gcttttttaa agaccgaagc gcttcggtaa cttctacggc cctgctgggt gtatggcata
     5641 tcgatcccga ctatcttcct acattgggaa tgaaactcgt cgccggcagg aatttctcgc
     5701 cgcagttgca caccgactcc gcctgtgcac tcgtcaatga gacggctgcg cggatgctgg
     5761 gctatgcgca tccgcttgga gaaaaggtct acagaggtcc tgactctaca tcggctcaca
     5821 ccattatcgg tgtggtgaag gatttcaata caggtacgct gcgcaacccg atcgatccgg
     5881 tgattttcgt tctggcaccg gacggtgtgg ccgtcacttt tcgattggcc ccgggggata
     5941 tcgcttccac gatgaatgcc atcaggcgcc ggtacgagac ggtagcgggc ggatatcctt
     6001 tcatctattc ttttctcgat gaggatttca accgccagta tgcggccgat caacgcaccg
     6061 ccggtctgtt taccgttttc tccggtctgg ccctgatcat tgccgccc
//
LOCUS       DASYSV010000067         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1810773, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000067 DASYSV010000000
VERSION     DASYSV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810773"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VGQ51_02020"
     CDS             complement(<1..989)
                     /locus_tag="VGQ51_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035361.1"
                     /translation="MKRLLFSTVILVAAGQVRAQSARPALSDIHSNKAVKHDVSASLS
                     DQRLSPDEDDDDSEGSDSGRIGPSRIGRGISQLEGRVGKPWNPAVSIEQTWFGSRPAP
                     ELLNSFDGLGDGFSGPQGTAFYRNPSDNALAVGPDHIFQIVNTRMAIFTKKGKKYDTT
                     GRVILGPAETRSVFKGFGGPCERMNNGDAVVRYDQLANRWLIVMPTFRRGIPRTEEVD
                     SAHGMKPWFHQPGEAEMMYQAPPTYVQERPFTGQRPSRTRVDTTGTFCMCYAISTGPD
                     PLGPYYRYEFDRPLFPDYPRPTIWPDGYYTTSSTSDNLIQRQAFVADRDKMLKG"
     gene            complement(1064..1843)
                     /locus_tag="VGQ51_02025"
     CDS             complement(1064..1843)
                     /locus_tag="VGQ51_02025"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEV9035362.1"
                     /translation="MLRIEGLRCLAGGKPVIQEITTGFAPGMLHVIVGPNGSGKSTFL
                     KAFSGEWPLAAGEITYDGEPLRQLTPTALSRRRAVMSQLPELHFPLRVDDIVMMGRYP
                     HFSFRPSRHDTGICRAAMERLSILNLAARDYLTLSGGERQRVQFARALTQIWDPPDKG
                     SRYLFLDEPVSSLDIRYQHQLLESVRVLVGQNIVIVAILHDLNLALQYADRILFLKEG
                     RIAAEGPAPEVVRPELIREVFGVESHVLQNPFGPQPVIVYG"
     gene            complement(1836..2903)
                     /locus_tag="VGQ51_02030"
     CDS             complement(1836..2903)
                     /locus_tag="VGQ51_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV9035363.1"
                     /translation="MKKNISFGAYYWGLAILLVLTLVVSARVGAVSIGFDRIFLFLRH
                     AVGFGRDGSAPSIEEQLFLQIRLPRVLLCALTGATLSVSGVLMQALFRNPIVEPGLAG
                     TSSGAAFGAAFVFVMGKSLTGAWVDALGPLLLPAFAFAGAGVATWLVYRVANVFGKVN
                     VNTLLLAGIAVNALAAGGTGFFSYVARDPQARSIVFWNLGTLSGADWQNTGIVAVTTV
                     GGIVLSLRYARALNALMLGETEAGYLGVRTQRLKSRVLLVNTVMVAVATSVVGVIGFV
                     GLVVPHILRLLKSSDNRFLIIASALLGAILLNLADMLARAIVAPSEFPIGVVTAFAGA
                     PVFLFLLIRAGRQQQEGGFYA"
     gene            complement(2900..>3300)
                     /locus_tag="VGQ51_02035"
     CDS             complement(2900..>3300)
                     /locus_tag="VGQ51_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV9035364.1"
                     /translation="RRTYAGEPPLRVLVIHFGQAANQYFVLSHRGIQQQMLEWAGAIN
                     AADTAQKWKNLSAEVIAEAQPDVILATDYGFDLQGSVEKFKQLPGVSLSPAAKHNRIY
                     RIEEHDLVYLGPRTGQNVYNLIRMIHQGAR"
BASE COUNT          729 a         1041 c          900 g          630 t
ORIGIN      
        1 cccttcagca ttttgtcccg atcggcaacg aaagcctgcc gctggatcag gttgtcgctt
       61 gtactgctgg tcgtataata accgtcgggc cagatggtgg gtcggggata atcggggaac
      121 aacggccggt cgaattcata acggtaatag ggacccaacg ggtcgggtcc ggtactgatg
      181 gcataacaca tgcaaaaggt gccggtcgta tcgacacgcg tccgcgacgg ccgctgaccg
      241 gtgaaaggcc tctcctggac ataagtgggt ggcgcctgat acatcatttc ggcttctccc
      301 ggctgatgaa accagggctt cattccgtgc gcgctgtcga cttcctccgt acgcggaata
      361 cccctgcgga aggtcggcat gacgatcagc caccggttcg ccagctgatc ataccggacc
      421 acggcatccc cattgttcat tcgctcacag ggcccaccga atcctttgaa aacgctgcgc
      481 gtctccgcgg gccccaggat cacgcgcccg gtcgtatcgt actttttgcc ttttttggta
      541 aatatcgcca tccgggtatt cacgatctgg aagatatgat caggccctac cgccagcgca
      601 ttgtcggaag ggttccggta aaaggcggtg ccctgcggac ccgaaaatcc gtcaccaagt
      661 ccgtcaaaac tgttcagcag ttcgggcgcc ggcctggacc cgaaccaggt ctgctctatc
      721 gatactgcgg ggttccacgg tttgccgacc cggccttcca attgcgaaat gccgcggcct
      781 atcctcgacg gaccgatccg gccgctatca ctcccttcgg agtcatcgtc atcttcatca
      841 gggctcagcc gctgatccga caaagacgcg gagacatcgt gtttgacagc tttgttgctg
      901 tgtatgtccg aaagagcggg cctggcggat tgcgcccgga cctgtcctgc ggctaccagt
      961 atgacggtgg aaaataagag acgcttcata ccttaaatgt ccttaacgtc ccataaatac
     1021 cctattcgga aattagccat ttcccgtttg aaattaaccc attctatccg tagacgatga
     1081 cgggctgcgg gccgaacggg ttctgaagca catgcgattc cactccgaat acttcacgga
     1141 tcaattccgg ccggaccacc tccggcgccg gcccctccgc agcgatacgc ccctctttca
     1201 gaaagagtat ccgatccgca tattgcaagg ccaggttcag atcgtggagg atggcgacga
     1261 tcacgatgtt ttgaccgacc agtaccctca ccgattccag cagctgatgc tgataccgga
     1321 tgtcgagact gctgacgggc tcatcgagaa aaagatagcg gctgccttta tcaggaggat
     1381 cccagatctg cgtcagcgcc cgggcgaact gcacccgctg ccgctcgcct cccgacaggg
     1441 tgagataatc cctcgctgcc agattaagaa tggacaatcg ctccatggcc gcgcggcaga
     1501 tcccggtatc gtggcgcgaa gggcggaaag aaaaatgcgg ataacgaccc atcataacga
     1561 tgtcatctac ccgaagcggg aaatgcagct cgggcagctg gctcatcacg gccctgcgtc
     1621 gcgaaagggc cgtcggggtc aattgccgca agggctctcc gtcgtaggta atctcgccgg
     1681 cggcgagagg ccactcgccg ctgaaggctt tcaggaaggt ggatttcccc gagccgttcg
     1741 gccccacgat cacatgcagc atccccggag cgaatccggt ggtgatctcc tgtatcaccg
     1801 gtttgccgcc cgccagacag cggagacctt cgatcctaag catagaaccc tccttcctgt
     1861 tgttgtctcc cggccctgat gagcaggaac agaaagaccg gggcgcccgc aaatgcggtg
     1921 acaacgccga tcgggaattc ggagggcgcg acgatcgccc gcgccagcat gtcggcaagg
     1981 ttcagcagga tagcacccag cagcgcggag gcgatgatca ggaaccggtt atccgagctc
     2041 ttcaacagtc ttaaaatgtg cggtaccacc agcccgacaa aaccgatcac gcctacgacc
     2101 gaggtcgcca ccgcaaccat gacggtgttg accagcagga cacggctttt cagacgctgt
     2161 gtccggacgc cgagataacc tgcttccgtc tctcccagca tcagcgcgtt cagcgcccgc
     2221 gcatatcgaa gagacaacac gatgcccccg acggtggtca ccgcaacgat cccggtattc
     2281 tgccaatccg cgcccgacaa ggtgcccagg ttccagaaaa cgatactgcg ggcctgcgga
     2341 tcacgcgcga cataggagaa aaagcccgtt ccacccgccg caagcgcgtt cacggcgata
     2401 cccgccagca acagcgtatt cacattcacc tttccgaata cattcgccac ccggtacacc
     2461 agccaggtcg cgacgccggc accggcaaaa gcaaacgccg gaagaagcaa gggtcccagc
     2521 gcatccaccc aagcccccgt gagactcttt cccatcacga agacgaatgc agccccgaac
     2581 gcggcgccgg aagacgtccc ggcaagaccg ggctctacaa taggattgcg gaacagcgcc
     2641 tgcatcaata ctcccgagac cgagagcgtg gcgccggtca gcgcgcaaag cagcacacgc
     2701 ggcagccgga tctgaaggaa tagttgctct tcgatggaag gagctgatcc gtcacgtccg
     2761 aaacctacag cgtgacgcag gaacagaaag atgcgatcga aaccgatcga taccgccccc
     2821 accctcgccg atacgacaag ggtcaggacc agcaatatcg ccagccccca ataatatgct
     2881 ccgaatgaaa tattcttctt catcgtgctc cctgatggat catccggatc aaattgtata
     2941 cattttgacc cgtccgtggc ccaagataca ccaggtcatg ctcttctatt ctataaatcc
     3001 gattgtgctt tgccgcgggg gacagcgaca cccccggcag ctgtttgaat ttttccacgc
     3061 tgccctgcag atcgaagccg tagtctgtgg ccaggatgac atccggctgc gcctccgcta
     3121 tgacctccgc gctcaggttc ttccatttct gcgcggtgtc cgcggcattg atcgcgccgg
     3181 cccattccag catctgctgc tggataccgc gatggctgag tacgaaatac tggttagcgg
     3241 cctgtccgaa atggatgacc agcacccgta gcggcggctc ccccgcatac gtgcgtcgca
//
LOCUS       DASYSV010000068        12495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_15822, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000068 DASYSV010000000
VERSION     DASYSV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12495
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15822"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VGQ51_02040"
     CDS             <1..466
                     /locus_tag="VGQ51_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035365.1"
                     /translation="ALPKPVTRSFWMAMLVFTFVNFSVYPTILKYQAGTQAGNYLSRA
                     DGADSTGPTAYLLQEAPVDYSFEFDCPRPVQRLTMDSLARTLTPSGKVLVFAPGSFAD
                     SLTRHGLHTTALASFPNFHISQLTREFVNYRTRASVLTPWSLFIVRRQPFAP"
     gene            528..1706
                     /locus_tag="VGQ51_02045"
     CDS             528..1706
                     /locus_tag="VGQ51_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEV9035366.1"
                     /translation="MSVQPFTISVSPFVIEDLRERLGRARWPDEIRDSGWQYGTNLSY
                     LRDLCIHWGHGFDWKEQEEALNAFHHYRAEVNGVGLHFIHERGKSHHTLPLLLIHGWP
                     DSFVRFLPLIPLLTAEGPDGLSFDVIIPSIPGFGFSDRPEEPGMNAERIARMFARLMK
                     DQLGYSRFICHGGDWGSSIAVQLARMFPDRLLGIHMTDVPVRGVLDLTELELSPAEKE
                     YLETGAQWTRAEGAYGMLQATKPQTLAYALNDSPIGLAAWIIEKFHTWTDHDGHLEQT
                     YSRDQLLTNLTIYWVTQTAGSSFRLYYETFHHPSSMAAGRIQVPAGICLAPREFGNAP
                     REFAERILDVRQWTILPEGGHFLAMEQPQRLADDIFRFAAILRDTGLPTPEPAFPLRL
                     "
     gene            complement(1759..2256)
                     /locus_tag="VGQ51_02050"
     CDS             complement(1759..2256)
                     /locus_tag="VGQ51_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV9035367.1"
                     /translation="MSTIKPLLKELEQEAQTTRKMLERVPTAYFTWQPHKKSMTVQQL
                     ATHIAELPSWITMAVTTDGLDFAANPYEHKELHNNAELMTYFEESLADGRSHLADTTE
                     DKLEETWTLRNGEQVYHVATKGEIVRMAYCQIVHHRAQLGVFLRLLDIPIPGSYGPSA
                     DEHEN"
     gene            2401..2688
                     /locus_tag="VGQ51_02055"
     CDS             2401..2688
                     /locus_tag="VGQ51_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035368.1"
                     /translation="MNQQETVHPASPDKKQVRKEIGDKLTGALADYKQELGEKKLATR
                     VKKVSKLISRDLLKVSKKQKKNGKKASSAKPAKKVAKVKDKKQAARKKETV"
     gene            2800..3072
                     /locus_tag="VGQ51_02060"
     CDS             2800..3072
                     /locus_tag="VGQ51_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035369.1"
                     /translation="MASFGELKPNTYYLIQVDADSDIELISVILHTRETVLLRSFLPQ
                     AEDFFKYQDEPIFKLVEEVDEETAQQLESLYPPATEEEYYEEEAEE"
     gene            complement(3083..3448)
                     /locus_tag="VGQ51_02065"
     CDS             complement(3083..3448)
                     /locus_tag="VGQ51_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEV9035370.1"
                     /translation="MAINIRDTDLKRTIGQLLRYGVLSSSAIVLTGGIVYLVRHGGQY
                     PQYHSFLGEPDKMRRPALMWKAMLRGEGRALIQIGLLVLIATPIARIIFSIFGYLLEK
                     DYLYTVITAIVLLVIVWNF"
     gene            complement(3452..4288)
                     /locus_tag="VGQ51_02070"
     CDS             complement(3452..4288)
                     /locus_tag="VGQ51_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV9035371.1"
                     /translation="MTVLVFTAIIFVGAVVAGLLGSLTGLGGGVVIIPLLTLVFKVDI
                     HYAIGASLVSVIATSSGAAAAYVKEGITNIRLGMFLELATTVGAIIGAWMATWLSTSI
                     IAILFGAILILSAILSFVRLADPAVTGKPGSLSQRLRLNSSFPTAEGEQPYFVKHVIG
                     GFFMMNIAGIISGLLGIGSGALKVIAMDNVMRVPFKVSTTTSNFMIGVTAAASVGIYL
                     KRGYIDPSLSMPVVLGVLAGAFAGSKILTTARTTRLKTLFAFVILVLAAEMIYNGFVH
                     NI"
     gene            complement(4354..5433)
                     /locus_tag="VGQ51_02075"
     CDS             complement(4354..5433)
                     /locus_tag="VGQ51_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9035372.1"
                     /translation="MRYSILFVAVVLFFAACTSDRAADKIKAKNEVAPAHYQLDTIEK
                     HGVASVIKLPGQLAAYEEVSIYPRVNGYVKSVLVDIGSQVSKGQLLMELEAPELLQAT
                     MEAREKYARSLADFSMSKEHYQRLLEAAQTAGAVSPLDLSTLKSRMMADSALSNAEKS
                     NWQVQQTMLSYLRVTAPFDGVITERNVHPGTLISASGKDKPVLELKQVAHLRLQVDIP
                     ENLAVNLKDKDSLTFYTSALPGTRMTGYISRKSMNVNTEFRSERMEADVMNKSGRLSP
                     GMYADVLVYSNGNPNGLTVPRSAVVTSTERKYVIVVRDGKTVKVNVSTGNESGGKVEI
                     VGAVAPGEQVITNANDEIKEGIALK"
     gene            complement(5430..8657)
                     /locus_tag="VGQ51_02080"
     CDS             complement(5430..8657)
                     /locus_tag="VGQ51_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9035373.1"
                     /translation="MIRAALKRPISVLVLFTGLLLFSVMAVRTIPIDIFPKLNLPVIY
                     VIESYGGMSPQQMEGFFSTRMMDQFLYVNGVRSIESKNIQGLTLIKISFYDNTNMAEA
                     SAQLALQVNRAAKFFPPGALPPQVIRFDASSLPVGQLVFSSKTKSLKEIYDLVATRIR
                     PMFASVRGLSAPPPFGANSRSIVVGVDPEKLRRYNISPDQVVEAIARNNSMTPAGNLR
                     IDSLMYVTTVNSLEDHVQEFEDIPIVTNGSSTVFIHDIATVSDASDVTVDYALINGKR
                     SVYIPVVKTADASTWDVVQDLKSRLPEMKNLMPDDVNISYEFDQSVFVINSVKSLMTE
                     GILGAILTGLMVLLFLRDWRSSLVVIITIPVSILSAILFLSLSGQTINMMTLSGLALA
                     IGILVDQATVTIENIHQHLEMGKPKRQAILEACEEISFPLLLILLCILAVFAPSFVMK
                     GVPKAMFLPLSMSIGFAMIISYIAAQTLVPVISNWLLKEEMFRYKHTGFHAHAGLALD
                     KKEVDEVGRHTRQDTHHSQENGFFQRIKMRLTSRLEKWMPKRKWIVPVYLLLSLGAAG
                     FCFAIIGKDLIPKTNNGELQVRIKEPDGTRLEKTERTVRGILNVLDSTVNGHVAVSSA
                     YVGLVPSSYGTSNLYVFNSGTNEAVMEVELDKDYKVDLDGLKDKLRETIHQVYPAVTV
                     SFEPIELTEKIMAQGASTPIEVRIAGKNFDNIKDYAERLTGNLKKIPYLRDIQIAQPL
                     HFPTISMHIDRYRLAQMGLSLNEVARSITDATSSSRFTEKVQWLDQKVAYTYQVQVQV
                     PEYIMNSIEQLKSISLVKGRSRPVLSDVAQFSIDTLPGEYDRSGPRRFLTVSANIDHK
                     DLGAATAAVRNAVADLGALPPGLITQVRGMSSLLTDTLDSLQAGLLVAIVVILLLLAA
                     NYQSFGLAISVLATVPAVLLGAMLMLLATGATLNLQSYMGIIMSMGVSVANAILIVTN
                     AESLRLEYKDPFRAACVSAGIRLRPILMTSMAMIAGMVPMASGIGEAGDQSAPLGAAV
                     IGGLAASTFAALFMVPLVYGWVRQKSSFQSVSLLPELSNQQTIPS"
     gene            complement(8666..10063)
                     /locus_tag="VGQ51_02085"
     CDS             complement(8666..10063)
                     /locus_tag="VGQ51_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9035374.1"
                     /translation="MVAFCTRKLYRAISILLLMPPLSLFAQQKTYTLAEFVDSAQRHL
                     PVLLQKRSLVDAAKAGVTDARHAFLPNSYLGAELTAGTDNSLPGSYISFGVIPSSSSG
                     VRASNNYQPALGNIGFFSNQYELYDFGLKKATVQNAQAFANLSQADLERETYLIKWQV
                     ARLYLDILKSQFQLSIDRENVHRYDTIYKVIQAVTRSGIKPGADSALAMAELSRTRTT
                     YNQTMGQVRQLQQQLSYLTGIPAGGIAIDTSKTNDYRTLLSGNRTDSLVTGNPLVDYF
                     DKERAMYLQREQLVRKSYLPRVLLNGVGWARGSSIDYTDQYKSSAISGLGYQRFNYMA
                     ALTVEYDLFNTVHRKDKALIARNNVAASEYGLQQQQLSLQNVGNKAEEGIKTASLNLI
                     EIPIQVRSAEDAFNQKTAQYKAGIINLVDLTNASYVLYSAQISYAETINDWFLANLDK
                     AAATGSLNQFIQLIK"
     gene            complement(10108..10482)
                     /locus_tag="VGQ51_02090"
     CDS             complement(10108..10482)
                     /locus_tag="VGQ51_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035375.1"
                     /translation="MPKRIHKYLFYTLIAVVYGVFFSVESFYNFEGHSEAKKLLSHAS
                     LVRLTDGNRGVSTSSARFPSSHSIRLNKRYQQENFTPCPIFRVEMPVCYVVPRTPRPY
                     PVCPLPFITIDHSSLRGPPIAA"
     gene            complement(10551..11237)
                     /locus_tag="VGQ51_02095"
     CDS             complement(10551..11237)
                     /locus_tag="VGQ51_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEV9035376.1"
                     /translation="MPVLIRAILSIIMKGFNAIVQIFLPKDKVFYSLFEEVADSVARM
                     GELLKEVVGEQDFDKRAALILQLEEQEHVNDDLTHRIFTELGRNFITPFDREDIHYLA
                     SALDDICDYIYAAGKKINFYKVNPNDPGIQKMADLIQQGANQICGAVKELRNMRNMRT
                     ITDALVRVNSIENQADDVFDMSIERLFAMEPDAKEVIKKRDIYRVMETVTDKCEDATN
                     VIESIIIKYA"
     gene            complement(11280..12389)
                     /locus_tag="VGQ51_02100"
     CDS             complement(11280..12389)
                     /locus_tag="VGQ51_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9035377.1"
                     /translation="MHYTFHIRPLTIVALCTAFSACGDHSKDGAQATSAIPAAQDTAQ
                     VFLLRSDTANKTVELPSELLPYLQTDLSAKVQGYVKEIKVDIGDQVHKGQTLAIIEAP
                     EVNTQVSQSEAALQAARAKYTASADKYHRLYEASQSASPGIVAPVDLISSHDQMLADS
                     AAFEVVRQQSKAYKEVSGYLHITAPFNGVITNRKADPGALVSAGTILLTVQDNNVLRL
                     RVAVPEIYVAAAAGRTSLSFAVDAYPEERFTGKLTRKTETIDPTTRTELWEFDVINNK
                     HLLKAGTFVSARLQLERNTPSFILPPSAIATTLERKFVIRVTDGKAQWVDVRQGMTTD
                     AGIEVFGDLHKSDTLLVKATDERKPGSSAVWKVVR"
BASE COUNT         2874 a         3387 c         3309 g         2925 t
ORIGIN      
        1 ggcgcttccg aaacccgtta cgcgcagctt ctggatggcc atgctggttt tcacattcgt
       61 caatttcagc gtttatccga cgatattgaa atatcaggcg ggcacacagg cgggcaatta
      121 tctctcccgt gctgacggcg ctgacagcac cggcccgacc gcctacttac tacaggaggc
      181 tccggtcgat tattccttcg aattcgattg cccgcggcca gtccaacggc tgacgatgga
      241 tagccttgca cgcacgctga cgccatccgg taaagtactg gtttttgcgc ccggcagctt
      301 cgccgattcg ctgacccggc atgggctgca cacgacagcc ctggccagct ttcccaattt
      361 ccatatcagc cagctgacaa gggaattcgt gaattaccgc acccgcgcat ccgttctgac
      421 gccctggtcg ctgttcattg tccggcggca accgtttgcc ccttagtcac ttacagtcga
      481 cgtgtagggc tggtcggatt tttgctgcgc ctagtagaaa atcacctatg agcgtgcaac
      541 catttaccat ctcggtttcc cccttcgtca tagaagacct gcgcgaacgg ttgggaagag
      601 cgcgctggcc cgatgaaatt cgggacagcg gttggcagta cgggaccaat ctgtcctatc
      661 tgcgggatct ctgcatacac tggggacatg gcttcgactg gaaagaacag gaagaagcct
      721 taaatgcttt tcaccactac cgcgcggagg tcaatggcgt cggacttcat ttcatacacg
      781 aaagagggaa gagccaccat acgcttccgc tgttattgat ccatggctgg ccggatagtt
      841 tcgtacgctt cctgcccctg atacccttgc tcacggccga aggtcctgac ggtctttctt
      901 tcgatgtcat cattccctcc atccccggtt tcgggttttc agacagaccc gaggaacccg
      961 gcatgaacgc cgagcgcatc gcccggatgt tcgcccgcct gatgaaggac caattgggat
     1021 attcacggtt tatttgtcat ggcggcgatt ggggaagcag catcgccgtg caacttgccc
     1081 ggatgtttcc cgaccgcctg cttgggatcc atatgacaga tgttccggtc aggggcgttt
     1141 tggatctgac cgagctcgag ctcagtcctg ccgaaaaaga atatctggaa accggtgctc
     1201 aatggacccg ggccgagggg gcatacggca tgctgcaggc gaccaagccg cagacacttg
     1261 cctatgcgct caatgattct cccatcgggc tggccgcatg gatcatcgaa aaattccata
     1321 cctggaccga tcatgacggt cacctggagc agacctattc ccgggaccag ctgctgacca
     1381 atttgaccat ttattgggtc acccagaccg cgggctcgtc tttccggcta tattatgaaa
     1441 cctttcatca cccttcgtcc atggcggccg gaaggatcca ggtaccggca ggaatctgcc
     1501 tggctccaag agagtttggt aacgcgccga gagagtttgc cgagcggatc ctggatgtac
     1561 gacagtggac catcctgccc gaagggggac atttcctggc gatggagcag ccgcagcgac
     1621 tggcggacga tattttccgg tttgcggcca tcctgcggga tactggtctg cccactcccg
     1681 aaccggcgtt ccccttacgt ttataattgc tacatgacgg gcgccgcccc gaaactgtca
     1741 gcgcggcgcc ccaacacctt aattctcatg ctcatccgca ctcgggccat aactcccggg
     1801 gatagggata tccaatagcc ggagaaagac acccaactgg gcccggtgat gaacgatctg
     1861 acaataggcc atccggacga tctccccctt tgtcgcgaca tgatagacct gctcgccgtt
     1921 acgcagggtc caggtttcct ccagcttgtc ctcggtagta tcggccagat gcgatcttcc
     1981 gtctgccagc gactcctcga aataggtcat cagttcggca ttgttatgca attccttgtg
     2041 ttcataggga ttagcggcaa aatcgagtcc gtcggtcgtc acggccatgg tgatccagct
     2101 gggcagctcg gcaatatggg tggccaactg ctgaacggtc atgctctttt tgtgcggctg
     2161 ccaggtaaaa tatgcggtcg gtacgcgttc cagcatcttg cgggtggtct gagcttcctg
     2221 ctccagttcc tttaacagcg gtttgatcgt tgacataaaa agtggttttg ttaagacaaa
     2281 gtaaccaccg ccccgtgaca gccttatgtc agcagcaaac ggcgacgaca caaaaaactt
     2341 aacattgggg attattatat taatattaaa tttaggttat cacatataac atttatctgt
     2401 atgaatcaac aagaaaccgt gcatcctgcg agtccggaca aaaaacaggt tcgtaaggag
     2461 attggggaca aactgacagg agccctggcc gactataaac aagaactggg ggaaaaaaag
     2521 ctggctaccc gcgtgaagaa ggtcagcaaa ctgatcagcc gcgatctgtt gaaggtcagc
     2581 aagaagcaaa aaaagaacgg caaaaaagca tcttccgcca aacctgccaa aaaggtggcg
     2641 aaggtcaaag ataaaaagca ggccgcgcgt aaaaaggaga ccgtataagc gatctcccct
     2701 ccgaatgaaa gagaccctgt aaagggtctc ttttttttaa catggacatt caacccgtgg
     2761 ccggctcgtt tgttaactta cagactaaat catcttacca tggccagctt tggagaatta
     2821 aagcccaaca catattacct gatccaggta gacgcagact cggatattga attgatctcg
     2881 gtgattcttc ataccagaga aacggttttg ctgcggagtt tccttcccca ggccgaggat
     2941 ttcttcaaat atcaggatga gcccatcttc aaattagtcg aggaagtaga cgaggagacg
     3001 gcccaacagc tcgaaagcct ttatccgccg gccaccgaag aggaatacta cgaggaagag
     3061 gcggaagaat gagctgcccc ggttagaaat tccacacgat gaccagcagt acgatcgccg
     3121 tgatcacggt gtacaggtaa tctttttcca ggaggtatcc gaagatggaa aaaatgatcc
     3181 gcgcgatggg cgtggcgatc agcaccagca gaccgatctg tatcaacgcc cggccttcgc
     3241 cccgcaacat cgctttccac atcagcgcgg gccgccgcat cttatccggc tcgcccagaa
     3301 aggaatggta ttgcggatac tggccgccat gccgcacgag atacacaata ccgccagtca
     3361 acacgattgc ggacgacgac agtacgccat accgcagcaa ctgaccaatg gtccttttca
     3421 agtcggtatc acggatattt atagccattt ttcaaatatt gtgtacaaac ccgttgtaga
     3481 tcatctccgc ggcaagcaca aggatcacga acgcgaacag cgtcttcagc cgtgtagtcc
     3541 tggcggtggt gagtatcttt gatcccgcaa atgcgccggc cagcacgccc aataccaccg
     3601 gcatggaaag gctgggatcg atatatcccc gtttcagata gatgccgaca ctggcggccg
     3661 cagtcacgcc gatcatgaaa ttgctggtgg tggtggatac cttgaacggc acgcgcatca
     3721 cattgtccat ggcgatcact tttagcgccc ccgagccgat gcccaataat ccggagatga
     3781 tccctgcaat gttcatcata aagaatccgc cgatcacatg cttcacgaaa taaggttgtt
     3841 cgccttctgc ggttgggaag ctgctgttca gccgtaatcg ctgagacaaa gaaccgggct
     3901 taccggtgac tgccggatcc gccagtcgta cgaaggacag gatggctgaa agaatgagga
     3961 tcgcaccgaa aaggatggcg atgatcgaag tcgacagcca cgtagccatc caggccccga
     4021 tgatcgcgcc gaccgtcgta gccagttcga ggaacatacc cagccggata ttggtaatgc
     4081 cttctttcac ataagcggcg gccgcacctg atgacgtggc gatcacgctg accagggacg
     4141 caccgatggc atagtggata tccaccttga agaccagggt cagcagaggg atgatcacca
     4201 cgcccccgcc aaggccggtg agcgaaccca ataacccggc taccaccgcc cctacaaaga
     4261 tgatagccgt aaaaacgagg acggtcatac aacaaatata gagcaataca acggtccccg
     4321 tgtcgtatag ccgacattca tggaaaacga tccttatttt aaggcaatgc cttcttttat
     4381 ctcatcgttt gcattggtga tcacttgttc gcccggcgcc acggcgccta ctatctccac
     4441 ctttccgccg ctttcgttgc cggtactgac attgaccttg acggtctttc cgtcccggac
     4501 cacgatgaca tatttccttt cggtggaagt cactacagcc gaccgcggca cggttaagcc
     4561 gttcgggttg ccattggaat agaccaggac gtcggcatac atgccagggg acaacctccc
     4621 actcttattc atcacatccg cctccattct ttcggaacgg aattccgtat ttacattcat
     4681 ggatttgcgg ctgatatagc cggtcatcct tgtgccgggc agcgcgctcg tgtaaaaagt
     4741 aaggctgtct ttatctttca ggttcactgc caggttttcc gggatatcca cttgcaggcg
     4801 caggtgagcc acttgtttca attccagcac gggtttatcc ttacccgagg cgctgatcag
     4861 ggtgccggga tgcacattcc gttcggttat cactccatcg aacggggcgg tgaccctgag
     4921 gtaggaaagc atcgtttgtt gtacctgcca gttgctcttc tccgcattgc tgagcgcgct
     4981 gtccgccatc atccgggatt tcagtgtgga cagatccagc ggcgaaacgg cgcccgccgt
     5041 ttgagcagct tccagcagac gctgataatg ttctttcgac atcgaaaaat cggccaggct
     5101 gcgggcatat ttttcacggg cttccatggt ggcctgtaac agctccggcg cctccagttc
     5161 catcaacagc tggcccttcg agacctgcga gccgatatcc accaacactg attttacata
     5221 gccgttcacc cttggataga tgcttacctc ctcataggct gccagctgac cgggaagttt
     5281 gatcaccgag gctaccccgt gcttctcgat cgtgtccagt tggtaatgag ccggtgcgac
     5341 ttcgtttttt gccttgattt tatccgctgc cctgtcggaa gtgcaggccg caaaaaacag
     5401 aacaacggcg acgaacagga tagaatatct catgatggaa tagtttgttg atttgaaagt
     5461 tcgggtaata aggaaacaga ttgaaaggat gatttttgcc tgacccaacc atagaccagc
     5521 ggcaccatga acagggcggc aaaggtggag gcggccaggc ctccgataac ggctgcgcca
     5581 aggggagcgg actggtcgcc cgcctcaccg ataccactgg ccatggggac catgccggcg
     5641 atcatagcca tgctcgtcat cagaatgggg cgcaacctta tgccggcgct cacgcaggcc
     5701 gccctgaatg ggtccttgta ctccaatcgc agcgattctg catttgttac gatcaggatg
     5761 gcattcgcca ccgacacccc catggacatg ataatgccca tgtaggattg gaggttcagg
     5821 gtggcgcctg tcgccagcag catcagcatg gctcccagca ataccgcggg taccgtcgcc
     5881 agtacggaga tcgccaggcc gaaggactgg tagttggccg ccagcagcaa caagatgacg
     5941 acaatggcga ccagcaggcc cgcctgcagg gaatccaggg tgtccgtcaa taacgaggac
     6001 atacccctga cctgggtgat cagacccggc ggcagcgctc ccaggtcggc taccgcgttc
     6061 cggactgccg ctgtggccgc gcccagatcc ttgtggtcga tattggcgct taccgtcagg
     6121 aaccgccggg gacccgaacg gtcatactcg ccaggcagcg tatcgatgct gaattgagcc
     6181 acatccgata ataccgggcg gctcctcccc ttaacgagcg aaatggattt cagctgttcg
     6241 atggaattca tgatgtattc cggcacctga acctgcacct ggtaagtata ggccactttt
     6301 tggtccagcc attgcacttt ttcagtgaaa cggctggaag aggtggcatc cgtgatgctc
     6361 ctcgccactt cgttgaggct taatcccatt tgcgccagcc ggtaccggtc gatatgcatg
     6421 cttatagtcg gaaaatgcag gggctgggcg atctgtatat cgcgcagata gggtatcttt
     6481 ttgagattgc cggtcagcct ttcggcgtaa tccttgatgt tgtcgaaatt tttgcccgcg
     6541 atccgcactt cgattggcgt agaggcaccc tgcgccatga tcttttccgt cagttcgatc
     6601 ggttcgaagg aaacagtcac agcaggatat acctgatgga tcgtttcgcg cagtttgtcc
     6661 ttcaacccat ccagatcgac cttataatct ttgtccagct ccacttccat aacggcttcg
     6721 ttggtgccac tgttaaaaac gtacaggttg ctggtgccat agctactggg caccaatccg
     6781 acatacgcgg agctgacagc tacatggccg ttcaccgtcg agtccagcac attcaggatc
     6841 ccccttaccg ttctttcggt tttttccagc ctggtcccgt ccggctcttt tatccggacc
     6901 tgcaattctc cattattggt tttgggtatc aggtcttttc caatgattgc gaaacaaaag
     6961 cctgcggcgc ccagggagag aaggagatag acaggaacga tccactttcg tttcggcatc
     7021 catttctcca ggcggctggt caaccgcatc ttgatacgct gaaagaaccc attttcctgg
     7081 gaatgatggg tatcctggcg ggtgtgccga cccacctcat ctacctcctt tttgtcaagc
     7141 gccaatccgg catgcgcatg gaagccggta tgcttgtacc ggaacatctc ttcttttagc
     7201 agccaattcg agatgacggg caccagcgtt tgggcggcga tgtaagatat gatcatggca
     7261 aaccctatag acatggacag gggaaggaac atcgccttgg gcactccttt cataacgaag
     7321 gaaggcgcga acaccgccag gatgcagagg agaatgagca atagcggaaa ggaaatctcc
     7381 tcgcacgctt ccaggatggc ctgtcgcttt ggcttgccca tttccaggtg ctgatgaatg
     7441 ttttcaatgg tgaccgttgc ctggtccacc aaaataccga tcgccaacgc cagcccgctc
     7501 aatgtcatca tattgatcgt ctggccggac aggctcagga acaggatggc gctcaggatg
     7561 gagacgggaa tggtaatgat cacgaccagg ctgcttcgcc aatctctcag gaaaagcagc
     7621 accatcagac cggtgagaat cgctcccagt ataccttcgg tcatcaggct ctttaccgag
     7681 ttgatcacaa atacagattg gtcgaactca tagctgatat tgacatcgtc cggcatcagg
     7741 ttcttcatct ccggtagcct ggacttgagg tcctgaacca catcccaggt tgaggcatcg
     7801 gctgttttta cgacagggat atagacggat cttttgccgt tgatcagcgc ataatccaca
     7861 gtgacatcgg aggcatcact gacagtggca atgtcatgta taaataccgt cgaagatcca
     7921 tttgtcacga tcgggatgtc ttcgaactcc tgcacatgat cctcgagaga gttgacggtg
     7981 gtcacataca taaggctgtc gatccggagg ttccctgccg gtgtcatcga attgttcctc
     8041 gcgatcgcct ccactacctg gtcgggtgaa atgttataac gccgcagttt ttccggatcg
     8101 acaccgacca cgatcgatct cgaattcgcc ccgaatggag gaggtgccga caacccgcgc
     8161 accgatgcga acatcggccg gatacgggtg gcgacgaggt cgtagatttc tttcagggac
     8221 ttggtcttgc tgctgaacac caactgtccg accggaagag aagaagcgtc gaaccggatc
     8281 acctgcggcg gtaatgcgcc gggtggaaag aacttggctg cccgatttac ctgtaatgcc
     8341 agctgggcag acgcctcggc catattggtg ttgtcgtaga acgagatctt gatcagcgtc
     8401 agcccctgaa tgttcttgct ctcaatgctt ctgacgccgt tgacatacag gaactgatcc
     8461 atcatacggg tcgaaaaaaa cccttccatc tgttgcggcg acatgccgcc ataggattca
     8521 atcacatata tgaccggcaa attcagtttg ggaaatatgt ctatcgggat ggtccggacg
     8581 gccatgacgg aaaacagtaa aaggcccgtg aagaggacga gtacgctgat aggccttttt
     8641 aaagctgcac gtatcatcag gtgtattatt tgatgagttg gatgaattga ttcagactac
     8701 ccgtggccgc ggccttatcc aggttggcca ggaaccaatc gttgatcgtt tctgcgtagg
     8761 atatttgcgc cgaatacagc acatacgaag cgttcgtcag gtcaacgagg ttgatgatgc
     8821 cggctttgta ctgggcggtc ttttgattaa aggcgtcctc ggcagagcgc acctggatcg
     8881 ggatctctat gaggttcagc gatgccgtct ttataccttc ctccgccttg ttgcccacat
     8941 tctgcagtga caactgctgc tgttgtagcc catactcgct ggctgccaca ttgtttcggg
     9001 cgatcaacgc cttatccttc cggtggacgg tattgaacag atcgtactcc accgtcagcg
     9061 cggccatata attgaaccgc tggtagccga gaccgctgat cgctgacgat ttgtattgat
     9121 cggtataatc gatactcgaa cccctggccc aaccgacgcc attgagaagc acacggggca
     9181 gataggattt tcggaccaac tgttcccgct ggagatacat tgcccgctct ttgtcgaaat
     9241 aatcgaccag cggattgccg gtcaccaggc tgtcggtacg gttccctgaa agaagggtcc
     9301 ggtagtcatt ggtcttagag gtatcgatgg cgataccgcc ggccggtata cccgtgagat
     9361 agctgagctg ctgttgcaat tgtctcacct gtcccatggt ctgattatag gtggtacgcg
     9421 tccttgacag ctcagccatc gccagcgccg aatccgcgcc cggtttgatc ccgctacggg
     9481 tcaccgcctg tatcacctta taaatggtgt cgtaccggtg tacgttctcc cggtcgatgg
     9541 acagctggaa ctggctcttc aggatatcga ggtacagcct ggcgacctgc catttgatca
     9601 gataggtctc ccgttccagg tcggcctgcg agagattggc gaatgcctgt gcattttgga
     9661 ccgtagcctt tttgagaccg aaatcataga gctcgtattg attggaaaag aaaccgatat
     9721 tgccgagcgc cggttgataa ttattcgaag cccgtacccc gctggaactc gatgggataa
     9781 cgccgaaaga gatatagctt ccgggtagcg aattgtccgt tcccgccgtc aactcggccc
     9841 cgagatacga attgggaagg aacgcatgcc gggcatccgt gaccccggct ttggcggcgt
     9901 cgacgagcga tctcttctgc agcaacaccg gtaggtgcct ttgggcgcta tcgacgaatt
     9961 cagccagtgt ataggttttt tgctgcgcaa aaagggaaag tgggggcatc agcagcagaa
    10021 tgctgatagc ccggtacaac tttctggtac agaaagcaac cataataata atatagattt
    10081 agatggaaga aagtttttac ggaaagatca ggcggcaatg ggaggcccgc gcagggagga
    10141 atgatcgatc gtaataaagg gtaatgggca aacaggatag ggccggggtg tcctgggcac
    10201 gacataacat accggcattt ccacgcggaa aatgggacag ggggtgaagt tctcctgctg
    10261 gtaccgtttg ttcagccgta tcgaatgcga ggacgggaat cgggccgatg aggtggaaac
    10321 gccgcggttg ccgtctgtaa gacggaccag cgaagcatgg ctcagtaact ttttggcttc
    10381 ggaatgcccc tcgaaattgt aaaaggattc aacggaaaag aatactccgt acacgacggc
    10441 gatcagtgta taaaacaggt acttatgtat tctttttggc atttgatgcg gcggaggcca
    10501 aaggtaggca gaaaaaatta catttctccg cggcggccca tcgggccgcc ctaagcatat
    10561 ttgattatta tggattctat aacattcgtc gcatcctcgc acttatcggt tacagtctcc
    10621 atcacccggt agatatccct ttttttgatg acctccttgg catccggctc catcgcaaaa
    10681 agacgctcga tgctcatgtc gaaaacgtcg tcggcctgat tctcgatgct gttgaccctc
    10741 accagcgcgt ccgtaatggt ccgcatattc cgcatgttcc gcagctcttt caccgcacca
    10801 catatctggt ttgccccctg ctggatcaga tctgccattt tctggatccc cgggtcattg
    10861 gggttcacct tataaaaatt gatctttttg ccggcggcat agatataatc gcagatgtca
    10921 tccaacgcac tggcgagata gtggatgtcc tcccggtcga aaggagtgat gaaattccgg
    10981 cccagctcgg tgaaaatgcg atgcgtcagg tcgtcattga catgctcctg ctcctcgagc
    11041 tgcaggatca gagccgcccg cttgtcgaaa tcctgctccc cgaccacttc ttttaatagc
    11101 tcgcccattc tcgcgacact gtccgccact tcttcgaaca gtgaatagaa gaccttgtcc
    11161 ttcggcagga agatttgtac gatggcattg aatcccttca taatgatcga caagatagcg
    11221 cggatgagaa ccggcatgcc cgacaccgac gattggtaat aatttcttaa cattagaccc
    11281 taacgaacca ccttccaaac agctgaactg ccgggttttc gctcatcggt agctttgacc
    11341 aacagggtat cgcttttgtg cagatcgccg aacacttcga tcccggcatc ggtcgtcatg
    11401 ccctggcgta cgtcgaccca ctgggccttg ccatccgtta cccgtatcac gaatttccgt
    11461 tccagcgtcg ttgcaatggc ggatggcggc agtataaagg acggggtgtt tctctccagt
    11521 tgaagacgcg ccgatacgaa agtgccggct ttcaataaat gcttgttgtt aattacatcg
    11581 aattcccaga gctccgtacg tgtcgtggga tcgatggtct ctgtcttccg ggtgagtttg
    11641 ccggtgaagc gttcttcggg atatgcgtca acggcaaatg acaggctggt acgccctgct
    11701 gccgcggcga cataaatctc gggaacggct acacgcaatc gaaggacgtt attgtcctgt
    11761 accgtaagaa gtatggtgcc tgcgctcaca agcgcacccg ggtccgcttt cctgttggtg
    11821 atgaccccgt taaatggagc ggtgatatgc agatatcccg agacttcctt atatgccttg
    11881 gattgctgac gcacaacctc aaaagcagcg ctgtccgcga gcatttgatc atggctgctg
    11941 ataaggtcta ccggcgcgac gatcccgggg gaggccgact gtgaggcctc atacagacga
    12001 tggtatttat ccgcgctggc ggtatatttc gccctggcag cctgtagggc ggcctcggac
    12061 tggctcacct gggtatttac ctccggagcc tcgatgatag caagtgtttg acctttatgc
    12121 acctggtcgc cgatgtccac ttttatttcc ttgacatagc cctgcacctt ggcagaaaga
    12181 tcggtttgca aataaggcag taattccgac ggaagctcga ccgttttgtt ggcggtatcg
    12241 ctccggagca ggaatacctg tgcagtgtcc tgtgcggccg gtattgcgga cgttgcctgc
    12301 gccccatctt tcgaatgatc gccgcatgcg gaaaaggcag tgcacaacgc aacgatagta
    12361 agcggtctga tatggaatgt atagtgcata gttcggatat agtagtgaat attattcatt
    12421 gtttgttacc aggatgtcgt cgggataaag cgaaacgggt ttatatgact ttcgtcccgc
    12481 caaccattga tagat
//
LOCUS       DASYSV010000069        13824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_142525, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000069 DASYSV010000000
VERSION     DASYSV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13824
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142525"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VGQ51_02105"
     CDS             <1..492
                     /locus_tag="VGQ51_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035378.1"
                     /translation="AMRARFKRGSDKFHVDSFDIGAFNDRAHSHLKANFTLQGYAKHL
                     GDEWFINMNLFKLYEHEEIDYPKRKMPIEMHFLNRRKYVVVLNIPDGYDVSYLPAGKT
                     YRNAIWGFKMTYEKKGKQLVFTQEFDNDHLMLQPDQFADWNKVLENLFPLYRETISLQ
                     KKS"
     gene            489..2450
                     /locus_tag="VGQ51_02110"
     CDS             489..2450
                     /locus_tag="VGQ51_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEV9035379.1"
                     /translation="MRNFLLALPLLLLTPPASSQNIVHEDWVANPVLHKLDARYSKES
                     AIILSDKRRIEYIDQPKDEIGEYRTLHKIIRVMDDNGIESFNKIYLGYNDPADIIAMR
                     ARTILPNGSVKEIRKEDMKDVQEEDGSKYKIFAMEGLEKGAEIEYFYTLKREADFFGH
                     ESLQGKFPIVDAEVDILCPDRLVFQMKGYNCALTVGDTSDGKTRTFSTRLGNIPGADE
                     EKYAAYEANLQRLEYRLSYNTAGGNEHVRLNTWNLLAQRIHEGYETNTEKELSRVGNF
                     IKDNGWDKLTDDRQKIVAVENFLKKNVTTREDIETRDAGNLEWIIKNKIATHRGIIRL
                     YAAIFNKLGIEHQIVLTCDRSEKSIDKSFENWNNASDLVFYFPSTKKFLAPTLLQMRY
                     PWINPYWGAEDALYCQTTTIGNYTTAIALVKPVPLEDFSQSFTRIDASIQFDKGLDSL
                     LIDLKHSFGGYGASGYRAAFTLGTPDQQHELLKDLVKSATNSERIVSSKIENAAFESY
                     SDNKPLIVQATVKSDGLLENAGNKLLVKIGEIIGSQVEMYQEKPRQFPVQVAYPQTEE
                     RFITFIIPDGYTIRNPGDLVIRHEYKEGDVVTMGFASDYKLDGRTLTVHILEQYRRTS
                     YPISQYEEFKKVINAAADFSKVSLILQKS"
     gene            complement(2488..4959)
                     /gene="priA"
                     /locus_tag="VGQ51_02115"
     CDS             complement(2488..4959)
                     /gene="priA"
                     /locus_tag="VGQ51_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV9035380.1"
                     /translation="MFAEVIIPLALPKNYTWSVPEQWQAAIREGCRVEVELRNKKYAG
                     IVKRLHEEKPGAFEPKPIGDLLDSDPIVHPAQLRLWEWIAGYYLCSEGEVMAAALPAH
                     FKLSSETILVFNPEAGDDLSFLDNDEFVVAEALQIKQELRLSDVQQLLDSTHVYPLIK
                     RLIEKKICFVWEALKDTYTAKTENFVILNPAYGSEEKLADLLNNWGRAPKQLELLLAW
                     LHLSKTEGDVTRAQLLKKSGASDAQLKGLVDKDILRIEKRTIARIRQLPRDIHIGFEL
                     TPAQQVAYDQTNAAFEKRPVCLLHGVTSSGKTQVYIRQIEQVIRQGKQVLYLLPEIAL
                     TSQIIRRLQQHFGGYIGIYHSKFSQNERLEIWNKIRKGEIKVVLGARSALFLPFDGLG
                     LIVVDEEHDASYKQQEPAPRYHARDAAIYYASLFGARVLLGSATPSVESYFNACSGKY
                     GLVALMERFGQVRLPEISIVDMKTLRAAMKGEKEFGGARDNGKPILSPSLHAAIEQTL
                     AANKQVILFQNRRGYSPYQVCEVCGWIPQCRNCDVSLNFHKLTNKLHCHYCGTVYPPL
                     QTCAACGNHHFVQRNFGTERIEEYLDEVFPDARIARMDIDSVRGKTAHDTLIQQFEQR
                     RIDILVGTQMVVKGLDFEHVGLVGILDADSILSFADYRVNERAFQLMEQVSGRAGRKD
                     GEGFVLIQVANTLHPVLGYVRAHDFGLFYQHEIEMRREFHYPPYSRVIRVTFRHKNRD
                     IVREAAQFFAGGLKKDFGAWLVGPAEPVVARVRNQYLYELLLKLPKDGQTIAFAKTVI
                     QQQTAIVQNARHWKSVVILPDVDAL"
     gene            complement(4972..5766)
                     /locus_tag="VGQ51_02120"
     CDS             complement(4972..5766)
                     /locus_tag="VGQ51_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV9035381.1"
                     /translation="MTPPTTPTMLKSLLRPLRWIYCSYALVLFIAGMFLVLPFVAFFA
                     LQGPKNGGDRIYRICRWWDLAWLTLVGIRRQLVYESVPDPARSYIFISNHISYLDIPM
                     ILRAVTRDELRVLGKAEMAKWPVFGYIYSRAVVMVDRSSMKDRSRSVRDLKTALTMGM
                     SVFIFPEGTFNETGRPLKDFYDGAFRIAIETQTPLQPILFLDTYDRMHYSGLFSLTPG
                     RTRLVFLPAVEVEGLRPSDVGQLKERVYRQMEEGLLHYRASWIKDR"
     gene            5855..7108
                     /gene="mtaB"
                     /locus_tag="VGQ51_02125"
     CDS             5855..7108
                     /gene="mtaB"
                     /locus_tag="VGQ51_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEV9035382.1"
                     /translation="MTSKTVAFHTLGCKLNYSETSSLSRMLTDEGFGKASFDDVADIY
                     VINTCSVTDNADKECRYLVRKVRRQAPGSMVVITGCYAQLKPEEIAGIPGVDLVLGAA
                     EKFNIAQHLKEWSKGDGAKICSCDIEGVTGFHSSYSLGDRTRTFLKVQDGCDYSCSFC
                     TIPMARGRSRSDTIKSTVSTVRHLAARGDVREIVLTGVNLGDFGAPPENLFGLMQALD
                     DIDGIDRYRISSIEPNLLTPDMIEWVAASRHFMPHFHIPLQSGSNRLLGLMRRRYRRE
                     LYAERVRLIKEVMPHCAIGADVIVGFPGETDEHFRETVDFLQELDVSYLHVFTYSERD
                     HTTALELTPVVPVAIRNERNKFLRSLSYRKMQEFIRRHEGQTRKVLLEGHVKTPMMEG
                     YTDNYIKITTPYREEWANRIVNWKL"
     gene            7195..7485
                     /locus_tag="VGQ51_02130"
     CDS             7195..7485
                     /locus_tag="VGQ51_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HEV9035383.1"
                     /translation="MRKFQVTIQFEMNDEFAGLVPSHRTYINRLIEQGVIDHYVVTME
                     TQRVWITFSAENRKAVEKYLSKSPLFKYWTYEIDELFMVDGLHYRLPVVQLN"
     gene            complement(7514..8071)
                     /locus_tag="VGQ51_02135"
     CDS             complement(7514..8071)
                     /locus_tag="VGQ51_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV9035384.1"
                     /translation="MENLSGYCKVVASMDKNNVHVHYHDKEYGFPIEDDNHLFARLIL
                     EINQAGLSWTTILNKKGNFFRAFDDFEIEKVARYGEKQRQRLLADAGIIRNRLKIDAT
                     IENAKKIRGIQKEFGSFRNWLDKHHPLSKEEWVKLFKNTFRFTGGEIVGEFLMSTGYL
                     PGAHVEGCPVYKKVLKHRPAWAKKG"
     gene            8115..10085
                     /locus_tag="VGQ51_02140"
     CDS             8115..10085
                     /locus_tag="VGQ51_02140"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV9035385.1"
                     /translation="MSRLPLFFLAGLLSSVALQAQSVSSPMGNPTTYDPQALFAPGFY
                     KDDHLATRSPNGAPSASYWQNRADYTLSVRLDTVRNELDGSAVIHYTNNSPDSLRSLW
                     LYLDQQTYRPDARSNSLTSAAAGGHTEGYRLSSVKIEQGGVTTDAEYIITDTRLQVRL
                     LHPLPARGGRVSLTIRYSYTVPGDFGNRTDFVSTRNGKIYEIAQWYPRMCMYDDRQGW
                     NTLPFLGAGEFYDEYGDFDYAVTVPWDMIVAGSGELLNPAAVLTSKQIARLAAARNSD
                     KTVMIRDAAAIGDPNSRPVHTGTLTWHFKMNNTRDVAFGASRAYIWDAARVNLPAGKH
                     SLAMSVYPVESAGPGAWDRATEYLKSSIEYFSKQWFAYPYPVAINEAGIAGGMEYPGI
                     VFDGITDKGKELYWVTAHEIGHNWFPMIVGSDERTYGWMDEGFNTFIDVYASDAFNNG
                     EYAPKRDDEYAPGGGNPVDEILPYLKDPQAPVIMTRADGVPEKYRHPMVYFKPALGLV
                     LLRERILGRDRFDYAFRRYIRSWAYRHPGPYDFFRTIENEAGEDLSWFWREWFFHNWS
                     LDLAVREVRITNGAAEITIENLDRMALPAILDLTWKDGTHERLTVPVETWLQGGTRVL
                     HVPATQPLVSVTIDPDHVLPDANRENDTWKTH"
     gene            10295..10480
                     /locus_tag="VGQ51_02145"
     CDS             10295..10480
                     /locus_tag="VGQ51_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035386.1"
                     /translation="MRILTKSQVAITGTDPAAYFWAGSSSGRASDAALGFATAANAFC
                     GGSTAGDFVLQNNDTIH"
     gene            10633..10710
                     /locus_tag="VGQ51_02150"
     tRNA            10633..10710
                     /locus_tag="VGQ51_02150"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10668..10670,aa:Val,seq:tac)
     gene            complement(12376..13281)
                     /locus_tag="VGQ51_02155"
     CDS             complement(12376..13281)
                     /locus_tag="VGQ51_02155"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEV9035387.1"
                     /translation="MRIERVNNLSEQDFLAEYLLPGRPVIVEDAMAGWDVASFQQEAL
                     LRKFGNCQVQVYNDLFDLQTIDRLEGYLKKNFNRTDGQSAHHYVRWYSRFKDVDFIWS
                     DEVFKALEDRWAHPYFLPDTSFVVPYSKGDDKVRINEKRYPYKGLFISGKGARTRLHR
                     DPLHSDAILCQFYGEKKVTLIPPSQEGLVMENGEVVDIQNPDLARFPRFRGAEIPYVD
                     VLAPGEVILFPAGWFHDVTCLSDSISVTWNFIHVSGAERFIQHLQDYPQDQQLEVVRF
                     FLRDELDAWATAEEIIEFVREKIII"
     gene            complement(13311..>13824)
                     /locus_tag="VGQ51_02160"
     CDS             complement(13311..>13824)
                     /locus_tag="VGQ51_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035388.1"
                     /translation="SVLAKNILYHMPNREAYVSPIGPWANSILCRKDIITGEIRYPHV
                     SLQEDSIFLNRLFGINCLIPVLAPWTYIYVYHGKNSWHSRHFRRLFGHSKKLPPAISA
                     LVTDVLEGKLDNAEASRLLMREEVLKEMDYFYFPPLAKRQIFANICQGSAYLLQRIVA
                     SVINFARRAS"
BASE COUNT         3254 a         3974 c         3473 g         3123 t
ORIGIN      
        1 gccatgaggg cccggtttaa aagaggctct gataaattcc atgtggattc cttcgacatc
       61 ggcgccttta acgacagggc gcatagccac ctcaaggcca acttcaccct gcagggctat
      121 gccaaacacc tcggcgacga atggttcatc aacatgaatc tcttcaaact ctatgaacac
      181 gaggagatcg actaccccaa acggaaaatg ccgatcgaaa tgcattttct caaccgccgg
      241 aaatatgtcg tcgtgctcaa tattcccgat ggatacgacg tcagctatct gcctgcggga
      301 aagacgtatc ggaatgccat ctggggcttc aaaatgacct atgaaaagaa agggaagcag
      361 ctggtcttca ctcaggagtt cgacaacgac cacctgatgc tgcaacccga tcaattcgcc
      421 gactggaaca aagtgctgga aaacctattc ccgctatacc gcgaaaccat cagtttacaa
      481 aagaaatcat gagaaacttt ttactcgccc tcccgttatt gctgctgacc ccgccagcaa
      541 gctcgcagaa catcgttcat gaggactggg tcgcaaatcc tgttcttcat aagctggacg
      601 cccgttatag taaagaatcc gctatcatcc tttccgacaa acggaggatc gaatatatcg
      661 accagcccaa ggacgagatc ggcgaatacc ggactctcca taagatcatc cgcgtcatgg
      721 acgacaatgg catcgagtcg ttcaataaga tatatctcgg gtacaatgat ccggccgata
      781 tcattgccat gcgtgcgcgg accatcctcc cgaacggatc ggtaaaagag atccggaaag
      841 aggacatgaa agacgtccag gaagaagatg gcagtaagta taagatattc gccatggaag
      901 gccttgaaaa aggcgcggag atcgaatatt tctatacact gaaacgcgaa gcggatttct
      961 tcggtcatga atccctgcag ggcaagttcc ccattgtcga tgccgaggtg gatatcctgt
     1021 gtcccgacag gctggtcttt cagatgaaag gctacaactg tgcgctcacc gtcggcgaca
     1081 cgagcgacgg caaaacccgg accttctcca cccggctcgg gaacatcccg ggtgcggatg
     1141 aggaaaaata tgcggcttat gaagccaatc tccagcgatt ggaataccgt ctttcctaca
     1201 ataccgccgg tggcaacgaa cacgtccggc tgaacacctg gaacctgctg gcacaacgca
     1261 tccatgaagg ctatgagacc aataccgaaa aggagctcag ccgggtagga aactttatca
     1321 aagacaacgg ctgggataaa ttgacggacg accgacagaa gatcgtcgcc gtcgaaaatt
     1381 tcctgaagaa gaatgtgacc acccgcgaag acatcgaaac gcgcgacgcc gggaacctgg
     1441 agtggatcat caagaacaag atcgccaccc atcgggggat catccggctc tatgcggcca
     1501 tattcaataa gttggggatc gagcaccaga tcgtgctcac ctgcgatcgg agcgagaagt
     1561 cgatcgacaa gtcgttcgag aactggaaca atgccagcga cctcgtcttc tactttccgt
     1621 ccaccaaaaa attcctcgct cctaccctgc tccagatgcg ctatccctgg atcaaccctt
     1681 actggggggc ggaggacgcc ttatattgtc agaccacgac catcggcaac tatacgaccg
     1741 cgatagccct tgtcaaaccg gtgccgctcg aagactttag tcaaagcttc acccggatcg
     1801 acgcttccat tcaatttgac aaggggctcg atagcctgct catcgatctg aagcacagtt
     1861 tcggcggtta tggcgcttcg ggataccgcg cggccttcac cctgggcacc cccgaccagc
     1921 agcacgagct gctcaaggac ctcgtgaaat ccgcgaccaa ctccgaaagg atcgtttctt
     1981 cgaagatcga gaacgccgcc ttcgagagct acagcgataa caaaccgctg atcgtccagg
     2041 caacggtaaa atccgatggg ttgctcgaaa acgcggggaa caagctgctc gtcaagatcg
     2101 gcgagatcat cggctcacag gtcgaaatgt accaggaaaa acccagacag ttccctgtcc
     2161 aggtggccta tccccaaacg gaggaaagat tcatcacctt catcataccc gatggctata
     2221 ccatccgcaa tccgggggac cttgttatca gacatgaata caaagaaggc gacgtggtca
     2281 cgatgggctt cgcctcggac tataagctgg acggcaggac gctcacggtc catattctcg
     2341 aacaataccg gcggacctcc tatcccattt cacaatatga ggaattcaag aaagtgatca
     2401 atgcggccgc ggatttcagt aaggtgtcgc tgatattgca aaagagctga acccttgcgg
     2461 ccacggccgg ccggcggcgg ccgcgactca caacgcatcg acgtccggca ggatcaccac
     2521 gcttttccaa tggcgcgcgt tctggacgat cgccgtctgt tgctgtatca cagttttggc
     2581 gaacgcgatg gtctgtccgt ccttcggcag cttgagcagc agctcataga gatattgatt
     2641 gcggacgcgt gccaccaccg gctcggccgg cccgacaagc caggcgccga agtccttttt
     2701 cagtcccccc gcgaaaaact gcgcggcctc tcgaacgata tccctgttct tatgccggaa
     2761 ggtcacacga atgacgcggc tatagggcgg ataatggaat tcacgacgca tctcgatctc
     2821 gtgctgatag aacagaccga aatcatgcgc gcgtacataa cccagcaccg gatgcagggt
     2881 attggccacc tggatgagaa cgaaaccttc accatccttt cgccccgcgc gaccgctgac
     2941 ctgctccatc aactgaaagg cccgctcgtt cacccggtaa tcggcgaatg acaggatgct
     3001 gtcggcatcc aggatgccta ccagcccgac atgttcgaaa tccagaccct tcaccaccat
     3061 ctgtgtcccg accaggatat cgatccgccg ctgttcgaat tgctggataa gggtatcgtg
     3121 ggcggtcttg ccgcgaacgc tgtcgatgtc catccttgcg atgcgggcgt cggggaagac
     3181 ttcgtccagg tattcctcga tccgctccgt cccgaagttt cgctgcacaa aatgatggtt
     3241 gccacaggcc gcgcaggtct gcagcggcgg atataccgtc ccgcaataat ggcaatgcag
     3301 cttgttggtc agcttgtgaa aattcaggga cacatcgcaa ttccggcatt gcggtatcca
     3361 gccgcagacc tcacagacct ggtaggggga atatccccgg cggttctgaa aaaggatcac
     3421 ctgtttgttc gccgccagcg tttgttcgat ggcggcatgc agcgacgggc ttaggatggg
     3481 tttgccattg tcgcgggcgc ctccgaattc cttctctccc ttcatggcag cccgtaacgt
     3541 tttcatatcc acgatcgata tttccggcag cctcacctgg ccgaaccgtt ccatcagcgc
     3601 cacgagccca tatttgcccg agcaggcatt gaaataggat tccacggaag gcgtcgcgct
     3661 tcccagcagt actcgcgccc cgaacaaaga ggcataatag atcgccgcgt cgcgcgcatg
     3721 gtatctcggt gcgggttcct gctgcttata ggaagcgtcg tgttcttcat cgacgacaat
     3781 gagccctaac ccgtcaaaag gcaggaaaag cgccgagcgc gcccccagga ccacttttat
     3841 ctcgcctttt ctgatcttgt tccagatctc gagtctttcg ttctggctga atttggagtg
     3901 atagatgccg atatatccgc cgaaatgttg ttgcagacgg cggatgatct gcgacgtcag
     3961 cgcgatctcc ggcaacaggt agaggacctg tttaccctgc cggatcacct gctctatctg
     4021 gcggatatag acctgcgtct ttccgctgga agttacgcca tgcaggagac aaaccggtcg
     4081 tttttcaaat gcggcattcg tctggtcgta ggcgacctgt tgtgctggtg tcagctcaaa
     4141 tccgatatgg atatcccgtg gcagctgccg gattcgggct atcgttcgtt tttcgatcct
     4201 caggatgtcc ttgtccacca accctttcaa ttgggcgtcg gaggctccgg attttttaag
     4261 cagctgagcg cgggtcacat cgccttctgt tttggagaga tggagccagg ccagcagtag
     4321 ttccagttgt ttgggcgcgc gtccccagtt gttgagcaga tcggccagtt tttcctcact
     4381 gccgtatgcg gggttcagaa tgacgaaatt ctccgttttg gcggtatagg tatcctttag
     4441 cgcctcccag acgaaacata tctttttttc gatgagcctt ttgataagag gatatacgtg
     4501 cgtagagtcc agcaattgct ggacatcgct gagccgcagc tcctgtttga tctgcagcgc
     4561 ttcggcgacg acgaattcgt cgttatccag gaagctgaga tcgtcgcctg cctccgggtt
     4621 gaagaccagg atcgtttcac tgctcagctt gaaatgcgcg ggcaaagcgg ccgccatgac
     4681 ctccccttcg ctgcacagat aatagcccgc gatccattcc catagccgca gctgggcggg
     4741 atgcacgatc gggtcgctgt cgagcagatc gccgatgggt ttgggctcga acgcaccggg
     4801 tttttcttca tgcagacgct tgacgatgcc ggcatatttc ttgttccgca gctcgacctc
     4861 cacgcggcaa ccttcgcgaa tggcagcctg ccactgctcc ggtacggacc aggtataatt
     4921 ctttggcaat gccagtggta tgataacttc cgcaaacatg aagcgtcaaa tttaccgatc
     4981 ttttatccaa ctggccctgt aatgtaataa cccctcctcc atctgccggt aaaccctttc
     5041 cttcagctgc cccacatccg acggcctcag cccctctacc tccaccgcag gcaagaacac
     5101 caggcgtgtg cgaccgggcg tcaacgaaaa aagacccgaa taatgcatcc ggtcgtaggt
     5161 atcgagaaaa aggatcggct gcagcggcgt ctgggtctcg atggcgatac gaaacgcacc
     5221 gtcataaaaa tctttgagtg gtcgcccggt ctcgttgaac gttccctcgg ggaagataaa
     5281 aacggacatg cccatggtta gagccgtttt cagatcgcgc acgcttcggg aacggtcctt
     5341 catgctgctg cggtcgacca tgacgacggc ccggctgtag atatacccga atacgggcca
     5401 tttggccatc tcggccttgc ccagcacgcg cagctcatcc cgggtcacgg cccggaggat
     5461 catggggata tccaggtaag agatatgatt ggaaatgaag atataagatc ttgcggggtc
     5521 gggcaccgac tcataaacca gctgccgccg gatgccgacg agcgtcagcc aggctaaatc
     5581 ccaccagcgg cagatgcgat aaatacgatc cccgccattt ttggggccct gcaacgcgaa
     5641 aaatgcgaca aagggcaata cgaggaacat ccccgcgata aaaaggacca gcgcatagga
     5701 acaatatatc caacggagag gccggagtaa ggatttcagc attgttggcg tcgtcggcgg
     5761 cgtcataatg tccgaagata cggtccgggc ttgtatttcc acgctttcac ggtatcttca
     5821 tatttccacc ctttcgcagt atctttgccg gctgatgaca tccaaaacgg tagcgttcca
     5881 caccctgggt tgtaagctca actattcgga gacctccagt ctttcccgca tgctcacgga
     5941 cgagggcttc ggaaaggcct cttttgatga tgtcgcggat atctatgtca tcaatacctg
     6001 cagcgtcacc gacaacgccg ataaggaatg ccgctacctg gtccggaaag tccgccgcca
     6061 ggcgcccggc agcatggtcg tcatcaccgg ctgctatgcc cagctgaaac ccgaagagat
     6121 cgccggtatt cccggtgtcg acctggtcct cggcgccgcc gagaagttca atatcgcgca
     6181 acacctgaaa gaatggtcca aaggcgacgg cgcgaagatc tgcagctgtg acattgaagg
     6241 ggtgacggga ttccattctt cctattctct gggagaccgg acacgtactt tcctgaaggt
     6301 gcaggacggt tgcgactaca gctgttcgtt ttgcaccatt cccatggccc gcggccggag
     6361 ccgtagcgat accattaagt cgactgtttc aaccgttcgg cacctggcgg cgcgcggaga
     6421 tgtccgcgag atcgtcctca ccggcgtgaa cctgggcgat tttggcgcgc cccccgaaaa
     6481 cctcttcggc ctgatgcagg cgctcgacga catcgacggc atcgaccggt atcggatctc
     6541 ttcgatagaa cctaatctgc tcacgcccga tatgatcgaa tgggtggccg cgagccgcca
     6601 cttcatgcca catttccaca ttccgctgca aagcgggagt aacagactgc tggggctgat
     6661 gcgccgccgg tatagaagag agctatatgc ggaaagggtg cggctgataa aagaagtcat
     6721 gccccattgc gccatcggcg cggatgtcat cgtcggcttc cccggcgaga cggatgaaca
     6781 tttcagggag acggtcgatt tccttcagga gctggacgtt tcttatcttc acgtcttcac
     6841 ctactccgaa agagaccata cgacagccct cgaactcacg ccggtggttc ccgtcgccat
     6901 tcgtaatgaa aggaataaat tcctgcgcag cctctcctac cggaaaatgc aggaattcat
     6961 ccgccggcat gaaggccaga cccgcaaagt actcctggaa ggtcatgtaa agaccccgat
     7021 gatggaagga tataccgata actatatcaa gatcaccaca ccgtatcgcg aagaatgggc
     7081 caaccgcatc gtcaactgga agctataggc aactcttttc cacgacaatt cgtataatta
     7141 tagaaggcca tatggcaccc ggcacaagga ttgcagttgc gccttgatga acgtatgcga
     7201 aaattccagg tcaccataca attcgagatg aacgatgaat ttgcgggatt ggttccctca
     7261 caccgcacct acatcaaccg cctgatcgag caaggggtca tcgaccacta tgtcgtgacc
     7321 atggagacgc aacgcgtatg gatcactttt tcggccgaga acagaaaggc cgtcgaaaaa
     7381 tacctctcca aatccccgct tttcaaatat tggacctatg agatcgacga gctcttcatg
     7441 gtcgatggac tacactaccg tctgcccgtc gtgcagttga actaacgctc tgcccgtgtc
     7501 gtcatcatcc gccctatcct ttcttcgccc atgccggccg atgcttcagc actttcttat
     7561 aaacaggaca accctccacg tgcgcacccg gcagataccc ggtactcatc aggaattctc
     7621 cgacgatctc gccacctgta aatcgaaagg tatttttgaa caacttcacc cattcttcct
     7681 tactcagcgg atgatgcttg tcgagccagt tcctgaacga cccgaattct ttttgtatcc
     7741 cgcgtatctt tttcgcattt tcgatggtcg cgtctatctt cagccggttg cggatgatgc
     7801 cggcgtcggc gagcaaacgc tgtcgctgct tttccccata acgcgccact ttttcgatct
     7861 cgaaatcgtc gaaagccctg aagaagttgc cctttttgtt caggatggtg gtccaggaca
     7921 gaccggcctg gttgatctcc aggatgagcc gggcgaacag atggttgtcg tcttcgatcg
     7981 ggaacccgta ttccttatcg tgatagtgca catggacatt gttcttgtcc atgctggcga
     8041 cgaccttgca ataaccagaa agattttcca taatcggggt ctgatagcgg gtaaatatac
     8101 tatagatttg ctgcatgtcc agacttcccc tgttcttcct cgccggcttg ctgtcctccg
     8161 tcgcgctgca agctcagtcc gtgtcttccc caatgggtaa tcctaccacc tacgacccgc
     8221 aagcgctttt cgcaccgggt ttttataagg atgatcatct cgctacgcgt tcgcccaatg
     8281 gcgctccgtc ggcatcttac tggcaaaacc gtgccgacta tacgctttcc gtccggctcg
     8341 ataccgtccg caatgagctc gatgggagtg ccgtcatcca ttataccaac aacagccccg
     8401 acagtctgcg atccctctgg ctctatctcg accagcagac ctatcggccc gatgcccgtt
     8461 cgaattcgct cacctcggcc gctgcaggcg gccacaccga aggctatcgg ctttcatccg
     8521 tgaagataga acagggaggc gtcaccacag atgcggaata tatcatcacc gatacacggc
     8581 ttcaggtgcg tctgctacac cccttgcctg ccagaggcgg acgggtcagc ctgaccatcc
     8641 gctacagcta tacggttccg ggcgattttg gcaaccggac cgattttgtg tctacgcgaa
     8701 acggcaagat ctatgagatc gcccagtggt atccgcggat gtgcatgtat gacgaccggc
     8761 agggctggaa taccctcccc ttcctcggtg ccggagaatt ctatgacgaa tacggcgatt
     8821 tcgactatgc cgtcacggtg ccatgggata tgatcgtcgc cggttccggc gaactgctga
     8881 atcccgcggc cgtcctcact tcaaagcaaa tcgcccgcct ggccgccgct cgcaacagcg
     8941 acaagacggt gatgatacgg gatgcggccg ccattggcga tccgaacagc cgccccgtcc
     9001 ataccggcac cctcacctgg cattttaaga tgaacaatac ccgcgatgtc gctttcggcg
     9061 ccagccgcgc ctatatctgg gatgccgctc gcgtgaacct cccggccggc aaacattcgc
     9121 tggccatgtc ggtatatccc gtggaaagcg ccggacccgg cgcctgggat cgcgccaccg
     9181 aatacctcaa gtcttccatc gaatattttt caaaacaatg gttcgcttac ccatatccgg
     9241 tagctatcaa cgaagcgggt attgccggcg gtatggaata ccccggcatc gtgtttgacg
     9301 gaatcaccga taaaggaaag gaactgtact gggtcaccgc ccatgagatc gggcataact
     9361 ggtttcccat gatcgtcggg tccgacgaac gtacgtatgg atggatggat gaaggcttca
     9421 atacatttat cgacgtatat gcttccgatg ccttcaacaa tggagaatat gctcccaaac
     9481 gcgatgacga atatgccccc ggcggcggca atcccgtcga cgagatcctg ccgtacttaa
     9541 aggacccgca ggcgccggtg atcatgacgc gcgcggatgg ggttccggag aaataccggc
     9601 atcccatggt ttatttcaag ccggcgctgg ggctagtatt gctacgcgaa cggatcctcg
     9661 gccgcgaccg cttcgactat gccttccggc gctatatccg gtcctgggcg tatcggcatc
     9721 cggggcctta tgattttttc cggacgatag agaatgaggc aggcgaagac ctgtcctggt
     9781 tctggcgtga atggttcttc cacaactgga gcctcgatct cgccgtgcgc gaggtgcgaa
     9841 tcacgaatgg cgccgcggag atcaccatcg aaaacctgga ccgcatggca ctgccggcta
     9901 tcctcgatct cacctggaaa gacggcacac acgagcggct gaccgtcccc gtcgagacct
     9961 ggctacaagg cggcacgcgt gtcctgcatg tccccgcaac acagccgctg gtgtcggtga
    10021 ccatcgatcc cgatcacgtg ctcccggatg cgaacaggga aaacgatacg tggaagacgc
    10081 attaaccgat cctcccccgc actactgctg cgttgtgcac caacgcgaaa gataacaacc
    10141 tgcacagtta taaacgaaag ctacacactt atcgctcgaa tcgtcacgcc tgtacattcc
    10201 tgtttttgcc ggatgaagct gggaccaacc caccaatttt gtcggcacaa cagacaacca
    10261 gccgctggtc ttcaagacca atgccaccga attcatgaga attcttacta aatcccaggt
    10321 cgcgatcacg gggacggatc ccgcggcata tttctgggcc ggcagcagtt ccggcagggc
    10381 atccgacgca gctctcgggt ttgcaacggc ggctaatgcg ttttgcggcg gttcgacggc
    10441 gggcgatttt gtccttcaga acaatgatac catccattaa gagccctgaa ttggactgag
    10501 cacgaatcga cctgagctcc gaaccggccg ggcgacggcc tgcccgaaaa accgacaaat
    10561 ttttttgggt gggcaccgtt ttactcctat ctttgccctc ctttcaacag attaccagtc
    10621 tgttaaggag acgggagttt agctcagttg gttcagagca tctgccttac aagcagaggg
    10681 tcggcggttc gaggccgtca actcccacca aatcaagcac cttttcggtg cttttttgtt
    10741 tttcgccaac gagggcgttg gcatcagcct tcaggatgat cctgttctcg aaaaccagca
    10801 catccatttc ggtatccagg aagcaatcga tcgcgtcctt tggcgtctct acgatcggcc
    10861 cgcccttgat gttgaacgac gtgttcagga gcacaggtac ccctgtcaac tggtaaaacg
    10921 agtcgatcag ccggtaatag tttggattgt caacagccgt tacggtctgt atcctggcgg
    10981 tcccatcgac atgtgttacg gcagggatat cctccttcag tacattgcat accctcagca
    11041 tgaaaggact gtccttcagt ccaaaccatt cttcggcatg ctcattcagc acgctgggag
    11101 caaatggcct gaacgcttcc cgaaacttca cctttttgtt cagcatgtct ttcattcccc
    11161 gattccgggg gtcggccaga atacttctgt tacccaacgc ccgggggcct acttcgctgc
    11221 taccctgaaa ccagccgatg atcttgccgt ccgccaggta ggaggcagtt ctgccgacaa
    11281 aatcctccgt cttttccact cgcagcttct ctttatattg cctgtgctcc agcacttcat
    11341 ccagcgtctg atcataagcg gctacggaat acatcatatc ccggttggaa aacattccct
    11401 tcggatgatc cagatgatcg taccaatacc atagcgccgc gccgatggcc gtcccatcat
    11461 ctccgctggc cggatggatg tgcaggcgct cgaacccgga ccgttcgaat cccaattggt
    11521 tggctactga gttcaatgct cctccaccgg ataaacaaag atgaagcgca aacttttccc
    11581 gaaccgccag gtcgtaaaga tccttcaata cactctccag ttgcatttcc aacagttgtt
    11641 gaatgttata tgccgctgtt gcattcagct tcttccccct ttcatccaga taaatggaac
    11701 gttcatttac cgagtagaaa ctcaagtcct ccaaggtcct gatgtcggca tacggtattt
    11761 tgacgttgct ttcggcaatg ccatacggtg caagccccat tacctttccc gctcctacga
    11821 tacccgtccc gaataacacc gtggcaaccg ttgcatacca gtcacaacta ttatacccct
    11881 tcaactggaa atcctgctta tatagcttgt tcttgcggcc aatgaacacg ccatatccta
    11941 cgggatccca cgaaaaacat atggcttctt caaaaggtga ggtctgatag acaagggaac
    12001 aatgcagcaa atgatgctcc gggtttatca aacatggaaa ttgtataccc cggatactga
    12061 ccttatccaa agtctgatta acccagaatt caagccagga ctcctttaag gaagtgccgt
    12121 acaggtacgg gatatccggc gaatccatat tgtggatatt gcacaaaagc acaatgccga
    12181 tatcacgtag gtccaccttc gcctgatcta atagagcatc cagatcggct tccctgatcc
    12241 cgggatgctt cttttttttc gaaaaccgct cggtattgac aaatgctgcc aatcttccat
    12301 tggagacaat tgcggccgac ccgtcatgat tgaagttgat cgctaaaatg tttgtgttaa
    12361 gcatttccca ttggtttaaa taattatttt ttcacgcaca aactctatta tctcttccgc
    12421 ggtcgcccac gcatccagtt catcccggag aaaaaatcga acgacttcca gctgttgatc
    12481 ctgcggataa tcctgaagat gctgaatgaa cctctccgct cccgagacat gtataaaatt
    12541 ccaggtgacg gatatactat cgctcagaca cgtgacgtcg tggaaccaac ccgccgggaa
    12601 cagaatgact tcacccggtg ccagcacatc gacatagggt atttctgcgc cgcggaaacg
    12661 ggggaaccgg gcgagatcgg gattttggat atccaccacc tcgccattct ccataaccag
    12721 cccctcctgt gagggcggga tgagcgttac ttttttttcg ccatagaatt ggcagaggat
    12781 ggcgtcgctg tgcaggggat ctctgtgcag ccgtgtcctc gcacctttgc ccgatatgaa
    12841 taaccctttg tacgggtagc gcttttcatt gatccttact ttgtcatcac ctttcgaata
    12901 aggtacgaca aaagaggtat cgggtagaaa ataaggatgc gcccatctat cctccagggc
    12961 tttaaaaact tcgtccgacc aaataaaatc gacgtccttg aaccggctat accaccttac
    13021 ataatgatga gcactctgcc catccgttct gttaaaattc tttttcaaat agccttccag
    13081 ccgatcgatc gtctgaagat cgaacaagtc gttgtatacc tgtacctgac aattcccgaa
    13141 tttcctcagc aaggcttcct gctgaaaact cgccacgtcc cagccggcca tggcatcctc
    13201 cacgataacc ggtctgccgg gaagcaaata ctccgctaaa aaatcctgct cacttaaatt
    13261 atttaccctt tcgattcgca taaaacaaag attgattgag tgaaattatt ttaggatgcc
    13321 cgtcgcgcga aattgatgac agaagcaaca atccgctgta gaagatacgc ggacccctga
    13381 cagatgttgg cgaagatctg ccgtttggca agtggcggga aatagaaata atccatctct
    13441 ttcagcacct cctcccgcat gagtaaccgc gaagcctccg cattgtccag cttcccttcc
    13501 agcacatccg ttaccagcgc gctgatggcc ggcggcaatt ttttactatg gccgaatagc
    13561 cggcggaaat ggcgggaatg ccagctattc ttcccatgat acacatatat atatgtccag
    13621 ggggcgagta ccgggatcag gcagttgatg ccgaacagcc gattcagaaa aatggagtcc
    13681 tcctgcaggg aaacgtgagg atatcttatc tccccggtga tgatatcctt ccggcacagg
    13741 atgctgttcg cccatgggcc gatgggagaa acataggcct cccggttcgg catatggtac
    13801 aaaatattct tggccagcac gctg
//
LOCUS       DASYSV010000070         7426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2192739, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000070 DASYSV010000000
VERSION     DASYSV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192739"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..496)
                     /locus_tag="VGQ51_02165"
     CDS             complement(203..496)
                     /locus_tag="VGQ51_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035389.1"
                     /translation="MLGLGCLCIYLLSSSPWATTLFAICADALLAIPTIGKAWRDPAL
                     ERSPAWVLGMISAALALIICFGHDWIYVLFPVYLLVFNGAMAWLTQMRVRLPG"
     gene            complement(569..778)
                     /locus_tag="VGQ51_02170"
     CDS             complement(569..778)
                     /locus_tag="VGQ51_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035390.1"
                     /translation="MVEKIIGVVAAIVIGMAQLTYLIDTIRKKIKPSVLSWLGWALLM
                     GTSVASQVATKGWQWSQASVLFSAF"
     gene            complement(788..1417)
                     /locus_tag="VGQ51_02175"
     CDS             complement(788..1417)
                     /locus_tag="VGQ51_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV9035391.1"
                     /translation="MANPSKTGSVKALFLDVGGVMLTNGWDGRSRQAAAKHFGLDIDE
                     LNDRHHLTFDTYESGKLSLDEYLQRSVFYQERSFSVADFRKFMFDQSVVLPGMIELVK
                     ALRAKYDLRIAVVNNEGRELNEYRIAKFGLNEFVDFFVSSCFVHFRKPDADIWKAALD
                     IAQVPREEVVYIDDRQLFVQVAEGLGIRSVWHKGVQATREILAGMGLVL"
     gene            1509..2543
                     /locus_tag="VGQ51_02180"
     CDS             1509..2543
                     /locus_tag="VGQ51_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV9035392.1"
                     /translation="MLPALSSKNVAAATAACLISLTLHAQKGSASDPYILNPSATTVA
                     WGYYWSEAKPVLSVHSGDYLTVRTLITSSPDRLEAAGVPPDQVEKELRDVQSVKERGP
                     GGHVLTGPIFIEEAEPGDVLEVKFNAIDLAIPYGYNAIGSAGFLSDEIFDRKMRIVPL
                     DAKKMIGHFAEGIDIPLHPFFGSVGVAPPKEAGRWNSAPPWVHAGNIDNKEMVAGTSL
                     FIPVFVRGALLEIGDGHAAMGNGEVDITAIETSLIGHLQLIVHKGKTQHWPRAETPTH
                     YISMGFDRDLNAATHIAVREMIAWLMDEKHLSRADAYMLCSVAGDVDITELVDGNVGV
                     HVMLPKNIFK"
     gene            complement(2594..3649)
                     /locus_tag="VGQ51_02185"
     CDS             complement(2594..3649)
                     /locus_tag="VGQ51_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035393.1"
                     /translation="MKKIFFGLAVSIAAVSLIVACNKSNSAGGNGTTTDGTTTTQLQT
                     QTDDETQVSTELDAATNDVNNTLNSVAALSGGTTTGFTGGVVVDGGNSGIVSNGSTGG
                     VITDGGSGSSVVAIDGICDARVTIDTANGLRVITITYNGNDCWVGHRRVRSGKVVITI
                     PVGVRWKDAGAVVTVSFVNLRVIRTRDHKTITLNGTYTYTNVTGGLLKDLPTLGTITH
                     TIAADSVTIRFADSATRIWSVAKQRVFTYNDGIVVTTTGTHSDGTNNDVSVWGTNRYG
                     NSFERMIVQPMVIAQSCDDRLTSGETETIRPAVTFTITYGLDVNGNPTTCPGSGYYYY
                     KLVWTKNGKTYTFIAPY"
     gene            3785..4891
                     /locus_tag="VGQ51_02190"
     CDS             3785..4891
                     /locus_tag="VGQ51_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEV9035394.1"
                     /translation="MRKTLHALTAAALLLPFALFAQRPLDSVLPVRGFCIDAPRPSGV
                     DSFVHFIDEELAPRKVNTLVLLVDYHYQFKSHPELIDSFALSDADVKKIVKVCARRHI
                     RIIPQINCLGHQGWEEHPGKLLQAYPEFDETPWVKNPVKYVWPNDDNLYCRSYCPLHP
                     RLHEVLFAAIDELCDAFECTAFHAGMDEVFYLGESKCPRCGGHDPAVLFAGEVRAIRD
                     HLAQKGRELWIWGDRLIDGKTTGIGMWEASYNNTFRAIDLIPKDVVICDWHYERPDQT
                     APYFALKGFRVVTCPWRVSANGVAQVDDMARYRDQSTREMKDRFLGIVETTWSPTRFF
                     LSGYYGLTPPNPSRPNTAWQCFRDVFQRIDALGN"
     gene            complement(4896..5084)
                     /locus_tag="VGQ51_02195"
     CDS             complement(4896..5084)
                     /locus_tag="VGQ51_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035395.1"
                     /translation="MFDSIHGVFTEGGPAFDGAGLFAKSHIQICIRNPNCIQGFFMPR
                     KEIDFIDWLEKKGLRAAA"
     gene            5395..>7426
                     /locus_tag="VGQ51_02200"
     CDS             5395..>7426
                     /locus_tag="VGQ51_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Helicase associated domain protein"
                     /protein_id="HEV9035396.1"
                     /translation="MDLFPHNREAYECATNLFKTGNRACIIQPTGTGKSVVIAEFVNR
                     NRSKRHLLLAPGSHIFDEIRKHLKSSKMSFSTYIGLKTNKSIFIENSFDFIYLDEFHR
                     LGAEIWGGGIRRLLRCNPRAKVLGTSATPIRYLDDNRDMASEIFDGRIAIQMSLNSAI
                     VKGILPSPTYVSAIYSLKEDLQLLKTKIASSRADNKKAILKDLNSKVIDWEKSSGLDT
                     VIQKHIDPHRKRVIVFCKNWEHLRDAQTLLDPIFKPIYGDFRSLCLYSKNKNAENESA
                     LNIFRENHEFPIVLYAIDKINEGLHSKNCNTVILFRNTTSPIVFYQQIGRAFSIKPAN
                     GPLILDLVNNFRNLQIATFKSDFERELEIASKERQASGKSDKKTTIRFIDETQDIRQI
                     FSLFEESIDNWTRLYAKAKLFFDQNGHLIVPAVGNMELYHWLNNQRKSYLAGSMSKER
                     IAKFNEIGMIFTPPLPLTFLRMQQKMEQWMQQHAELPTQSTNKKLAMWISNQRKDYKQ
                     GKLTEEQIAILQRFFPMEGDRTWLKIQACIDKVVAHFKQGNIDTADKRIRMDLLNLRN
                     LYNCDRLPQSALTQLREARVPVEVTINDFIWFENVKKILAWYEQNERLPSREDCQPLH
                     SFWMREKRYINNSHPYAKYLDFDREAGSWLQKLQTTISAEDPCVWNEQYLALR"
BASE COUNT         1873 a         2053 c         1773 g         1727 t
ORIGIN      
        1 cggcgcatac tatgtcgcct ggatcggttt tctgatgcac cacattctcc agactggcgg
       61 gtttcactga taccactggc tattaccact tccatgccaa accgaaattt tcggcgggat
      121 cgccgcggaa tcaggtgctc tcttatacgt ccgcgccgcg cggacgtatt tgcgtcagcc
      181 agccattgca cacgcatctg ggtcagccag gcagtcgcac ccgcatctgc gtcagccacg
      241 ccatcgcacc gttgaaaacc aacaaataga cgggaaacaa tacatagatc cagtcatgcc
      301 cgaaacaaat gatcagggca agcgcggccg agatcatccc caacacccac gcgggtgatc
      361 tttccagggc cggatcacgc caggctttgc caatggtagg tatggcgaga agcgcgtcgg
      421 cgcagatcgc gaagagggta gttgcccacg gactgctgga tagcaggtag atgcaaagac
      481 agccgagacc cagcaccagg aacgaccagt cttttcgcag cagcgaaaaa ttgccgctga
      541 aaaaagctac cgccgcgatc gccagacatc agaaggccga aaaaaggaca ctcgcctggc
      601 tccattgcca gcctttggtc gccacctgcg atgccacgct cgtacccatc agcaaagccc
      661 agcccagcca ggaaagcaca ctgggcttta tctttttccg gatcgtatcg atcagatagg
      721 tcaattgcgc cattccgatc acgatcgccg cgacaacgcc gattattttt tctaccatcg
      781 ccatgcttta caaaaccaat cccattcccg ccaatatttc acgcgtcgcc tgcacgccct
      841 tatgccacac acttcggatc cccaggccct ccgccacctg cacgaacaac tgccggtcat
      901 cgatatacac cacttcctcc cggggcacct gggcgatgtc cagcgccgcc ttccagatat
      961 ccgcatccgg cttccggaaa tgaacgaaac aagaactcac gaaaaaatcg acgaattcat
     1021 tcaacccgaa tttcgcgatc cggtactcat tcaattccct cccttcatta ttgaccaccg
     1081 caatccggag atcgtatttc gcccgcaatg ccttgaccaa ttcgatcatc ccgggaagga
     1141 cgacagactg atcgaacatg aattttcgga aatccgccac tgaaaaagat cgctcctggt
     1201 agaagaccga tcgctggagg tattcgtcca ggctcagctt gcccgactca taggtgtcga
     1261 aggtcaaatg atgccggtca ttcagctcat cgatatccaa accgaaatgt tttgccgcgg
     1321 cctgtctcga ccttccgtcc cagccattgg tcagcattac cccgccgaca tcgaggaaca
     1381 gggcttttac gctgccggtt ttcgaaggat ttgccattgc tgcgagttgt tattgtaacg
     1441 gcaatttaaa gcaaaggagg cgcaaccgcc taaatttgca tatcttgttt tcagaaaatt
     1501 gaccagccat gcttcctgct ctctcgtcca aaaatgtcgc ggccgccacc gccgcctgcc
     1561 tgatatcgct gacccttcac gcccaaaagg gatccgcgtc ggatccctat attttgaatc
     1621 cctccgccac gacagtggcc tggggatatt actggtccga agcgaaaccg gtgctcagcg
     1681 tccattccgg ggattatctg acagtccgca cactcatcac gtcgagcccc gaccgcctgg
     1741 aagctgcggg agtccctccg gaccaggtgg aaaaagaatt gcgcgacgta caatccgtga
     1801 aggagcgcgg acccggcggc catgtgctaa cagggccgat ctttatcgaa gaggctgaac
     1861 cgggagatgt actcgaggtc aagttcaatg ccatcgatct ggccattccc tacggctaca
     1921 atgccatcgg aagtgcagga tttctatccg atgagatctt cgaccggaaa atgcggatcg
     1981 tcccgctcga cgcaaaaaaa atgatcgggc atttcgccga aggcatcgat ataccgctac
     2041 atcccttctt cggaagcgta ggcgtcgccc cgccgaaaga agcgggtcgc tggaacagcg
     2101 ctccaccatg ggtccatgcc ggaaacatcg acaataaaga gatggttgcc ggtacttcgc
     2161 tattcatccc cgtattcgtc aggggcgcgc tgctggagat cggcgatggc catgccgcca
     2221 tgggcaatgg cgaggtagat attacggcga ttgaaacctc gctcatcggc catctgcaac
     2281 tgatcgttca caaaggcaaa acccagcact ggcctcgtgc cgaaactcct acccactaca
     2341 tcagcatggg tttcgacagg gaccttaacg cggccacgca tatcgccgtc cgcgaaatga
     2401 tcgcctggct gatggatgaa aaacatctgt cccgcgccga tgcctacatg ctctgcagtg
     2461 tagccggtga cgtcgatatc acggaactcg tcgacgggaa tgtcggagtg catgtgatgt
     2521 tgccgaagaa catcttcaaa taataaggcc gccccgcgca agcgcggggc ggccccaggc
     2581 tctattgtgt gactcaataa ggcgctataa acgtatacgt cttcccgttc ttcgtccaga
     2641 ccagtttgta ataatagtaa ccgcttcccg ggcatgtcgt cggatttcca ttgacgtcca
     2701 acccataggt gatggtgaag gtgacggcgg gacggatggt ctctgtctca ccgctggtca
     2761 gccggtcgtc gcaggattgt gcgatcacca tcggctgcac gatcatcctc tcgaaagagt
     2821 tcccataccg gttggtgccc cataccgaaa catcgttatt ggtgccgtcg gaatgtgtgc
     2881 cggtggtagt gaccacgatg ccgtcattat aggtaaagac tcgctgtttc gctacgctcc
     2941 agatccgggt ggcgctgtcg gcaaaccgga tggtcacgct gtcggccgcg atcgtatgcg
     3001 tgatcgtccc cagcgtgggc aggtccttta acaatccgcc ggtaacattc gtataagtat
     3061 aggtgccgtt cagtgtgatg gtcttgtggt ctctggtacg gatgaccctc aggttcacga
     3121 agctgaccgt gacgaccgcg cccgcgtctt tccaccgcac tccgacagga atggtgatca
     3181 cgaccttgcc gctgcggaca cgcctgtggc cgacccagca gtcgttacca ttataagtaa
     3241 tggtaatgac ccggagaccg ttcgccgtat cgatggtgac ccgcgcatca cagatgccgt
     3301 cgatggcaac gaccgaactc ccactgccgc cgtccgtgat gaccccgccg gtactcccgt
     3361 tcgaaacgat cccggaattg ccgccgtcga cgaccacccc gcccgtaaac ccggtcgtgg
     3421 ttccgccgct aagcgccgca acgctattca atgtattgtt cacatcattg gtcgcggcat
     3481 ccagttcggt ggacacctgt gtctcatcat ccgtctgcgt ctgcagctgt gtggtcgtgg
     3541 ttccatcagt cgtagtacca ttgccgccgg ccgaattgct tttgttacag gcgacgatca
     3601 ggctgacggc cgcaatacta accgccaggc caaagaatat ttttttcatg tgttgtgatt
     3661 tttaatctca gattaatttc gacgtgcgag gtttaacaga aacttatata ttctttttgt
     3721 ataaaatatt tcgaacggcg attaaactta tcggtttccg gccggtctgt tgtaaattca
     3781 gcctatgaga aaaacactgc acgcgctaac cgcggcagct ttattgctgc ctttcgccct
     3841 tttcgcgcaa cgaccgctgg acagcgttct gccggtacgg ggattttgca tcgatgcgcc
     3901 ccgcccttcc ggcgtagatt cctttgtgca ttttatcgac gaagagttgg cgccgcgcaa
     3961 ggtcaatacc ttggtccttc tcgtagatta ccattatcaa ttcaaatccc atcccgaact
     4021 gatcgacagc ttcgcactgt cggatgcaga tgtaaaaaag atcgtaaagg tctgtgcccg
     4081 ccggcatatc cgcatcatcc cgcagatcaa ttgcctgggt caccagggct gggaagaaca
     4141 tcccgggaaa ttattacagg cctatcccga attcgacgag accccgtggg taaaaaatcc
     4201 ggtgaaatat gtatggccca atgacgataa tctctattgc cgtagctatt gtccgctgca
     4261 tccccggctg catgaagtgt tgttcgccgc gatcgacgaa ttatgcgatg cctttgaatg
     4321 cacggccttt catgccggca tggacgaagt cttctatctg ggtgaaagca aatgcccccg
     4381 ttgcgggggc catgatcctg ctgtattgtt cgccggagaa gtgcgggcga tccgcgacca
     4441 ccttgcgcaa aagggccgag agctatggat ctggggcgac aggctcatcg acggcaagac
     4501 tacgggcatc ggcatgtggg aagccagcta taacaatact ttccgcgcca tcgatctcat
     4561 cccgaaagac gtcgtcatct gcgactggca ctacgaacgt cccgatcaga ccgctcccta
     4621 cttcgcgctg aaggggttcc gcgtggtcac ctgcccctgg cgcgtgtcgg ccaatggcgt
     4681 cgcacaggta gacgacatgg cgcgctatcg tgatcagtcc acgcgcgaaa tgaaggaccg
     4741 cttcctcgga atcgtcgaga ccacctggtc ccccacccgc ttcttcctca gcggctatta
     4801 cgggctgacg ccccccaacc cctcgcgacc caatacggcc tggcaatgtt tccgcgacgt
     4861 ttttcagcgt atcgatgcat tggggaatta gataattaag ccgcggctcg caaccctttc
     4921 ttttccagcc aatcaataaa atcaatttct ttccttggca tgaaaaatcc ctgaatgcag
     4981 ttggggttgc ggatgcagat ctgaatatgg cttttcgcaa ataatcccgc cccgtcgaac
     5041 gccgggcctc cttctgtaaa cacgccatgg atggaatcaa acatcttata ttcactatgc
     5101 cccctttcgg ccttaccgca gccgcataac tatgaaataa aattttaagt ccttcccaaa
     5161 aacttatgga tatcgaaaaa atagtcattc cccttataca tgattactct tatccgatag
     5221 atttttccgc ggaattcaaa tttctacgct attttgccac gtaattattt gaccaggctc
     5281 attcgcccat taaaccttac caaaatgtac ctatctacgc tttccttgtt cattttctcc
     5341 gccatgcacg cttaataagt aagcgtttct gttcatcacg tttaattatc ttatatggat
     5401 ttgtttccgc ataaccggga agcatacgaa tgtgccacca atttatttaa gacgggcaac
     5461 cgcgcttgca tcatacagcc tacgggcaca ggtaaaagcg tcgtcatagc cgaattcgta
     5521 aatcgcaaca ggtccaaaag gcatctgttg ctggcccctg gatcgcatat tttcgatgag
     5581 attcgaaaac atttgaaaag ctcgaaaatg tccttttcca cttatattgg cttaaagact
     5641 aacaaatcga ttttcatcga gaactcattc gatttcatct atctcgacga attccatcgt
     5701 ctcggcgcgg aaatatgggg tggcggtatc agacggctat taaggtgcaa tccaagggca
     5761 aaagtcctcg gcacgtcggc cacacccatc agatatcttg acgataaccg ggatatggca
     5821 tccgagatct ttgacggacg aatcgctatc caaatgtcgc tcaattcggc aatcgttaaa
     5881 ggaattcttc cttcaccgac atatgtaagc gccatttatt ccctaaagga ggacctacag
     5941 ctattaaaaa ctaaaatagc ctcttcgcgt gcagacaaca aaaaagcgat cctgaaggac
     6001 cttaacagca aggtcatcga ttgggagaag tcttccggcc tggacaccgt cattcagaag
     6061 cacatcgacc cccaccggaa aagggtgatc gttttctgta aaaactggga acacctgaga
     6121 gatgcccaaa cgctgctcga tccaattttt aaacccattt atggcgactt ccgaagccta
     6181 tgtctctatt ccaaaaataa aaatgcagaa aatgagtcgg ctctgaatat tttcagagaa
     6241 aatcatgaat tcccgatcgt gctatatgcg atcgataaga tcaatgaagg cctccattca
     6301 aaaaattgca acacggtcat cttgtttagg aacacaacat ctcccatcgt attttaccag
     6361 cagatcggca gggctttttc tatcaagccg gcaaatggtc cgctgatcct cgacctggtt
     6421 aacaatttca ggaatcttca gattgcaaca tttaaaagcg attttgagcg ggaattggaa
     6481 attgcatcga aagagcgaca agcatccggg aaaagcgata aaaagaccac gatcagattt
     6541 atagatgaga ctcaggatat acggcagatc ttctcgcttt tcgaagaaag tatcgataat
     6601 tggacgagac tatatgcgaa ggcgaagcta ttttttgacc aaaacggtca cctcatcgta
     6661 cccgccgtcg gaaatatgga actgtatcac tggttaaata accaacgaaa aagctatctg
     6721 gccggaagta tgagcaaaga gcggattgcc aaatttaatg agatcgggat gattttcact
     6781 cccccacttc ccttaacttt tttaagaatg cagcagaaaa tggaacagtg gatgcagcaa
     6841 cacgctgaac tacctaccca atcgacaaac aagaagttgg caatgtggat cagcaaccaa
     6901 cgcaaggact ataagcaggg caaactgacg gaggaacaaa tagccattct tcaacgattt
     6961 tttcctatgg agggagacag aacctggctg aagatccagg cctgtatcga caaggtcgta
     7021 gcgcacttca aacaaggcaa catcgatacg gctgacaaaa ggatcaggat ggaccttctt
     7081 aacctgcgga atttatataa ttgtgaccga ttacctcaaa gcgcattgac ccaattgcgg
     7141 gaggcgcgtg ttccggtgga agtgacgatc aatgacttta tctggtttga aaatgtaaaa
     7201 aaaatcctgg catggtacga acaaaatgag cgactgcctt cccgggagga ttgccaacca
     7261 ctgcattctt tttggatgag ggagaaaagg tatattaaca attcccatcc ctatgcaaaa
     7321 tatctcgatt ttgatagaga agccggaagc tggctccaaa agctgcaaac tacgatctcc
     7381 gcggaggatc cttgcgtttg gaatgaacag tatttggcac ttaggc
//
LOCUS       DASYSV010000071         7131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1938298, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000071 DASYSV010000000
VERSION     DASYSV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938298"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1825)
                     /locus_tag="VGQ51_02205"
     CDS             complement(<1..1825)
                     /locus_tag="VGQ51_02205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEV9035397.1"
                     /translation="MSISQDQLRRRFQEEYQQLNEQQRLAVDNTEGPVMVIAGPGTGK
                     TQILAARIGKILLDTDVFPGNILCLTYTEAGAIAMRRRLLSFIGPDAYKVHIHTFHSF
                     CNDIIQENLSLFEKTSLDPISDLEKIQLFKALIDRFPKNHPLKRYRGDVYFEMNNLQN
                     LFSTMKREGWNPTFIKEKIDQYLASLPDREEYIAKRATASFKKGEVRTDKIAEETERM
                     EKLRAAVDEFDRYQELMRVHNRYDFDDMINWVIRAFGENKPLLSSYQERYQYILVDEY
                     QDTSGVQNRLVNLLISYWEQPNVFVVGDDDQSIYRFQGANVENMLEFAQQYQKDLLTV
                     VLTNNYRSTQPILDVSRTLIQRNQERLVRQIPGLSKHLLASHPRTRQLRYPPRLLEYL
                     SPRQEMTGITLRVEQLLAKGISPGHIAIIYKENRYGEELARFLQLKNIPVYSKRSLDL
                     FGIALAQQIILLLRYLAAEHDAPYGGDEMLFEILHFHFFGIAPIEIAKLSVEVADKQY
                     NEEQRTSIRRLLYEKAQRPARDLFDTGIPEGLKKASRALEKLIGDVPNTTLQALFENV
                     IRDTGILASIVSSPDRIWQMQVLTALFDFVKEETRRNPDLDL"
     gene            1988..3349
                     /locus_tag="VGQ51_02210"
     CDS             1988..3349
                     /locus_tag="VGQ51_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV9035398.1"
                     /translation="MKNHHSFKVFIVEDDVWYGSMLQHYLALNPEYEVKRFESPRDFF
                     AQLHENPDVVTLDYSLPDCDGAEVLKKIKEHNPDIRVIVISGQEDVATAINLLKNGAF
                     DYIVKDDDTKDRLWNSILHLREISNLKQEVETLKSQVSRKYDFSQMIIGKSEQIQKVF
                     SMIEKASKTNITVSITGETGSGKEMIAKAIHYNSDRHKLPYVAVNVAAIPRDLIESEL
                     FGHEKGAFTGAVTRRIGKFEEADKGTLFLDEIGELDINLQAKLLRVLQEREITRIGAN
                     GITKIDVRIVVATHKNLLDEVKNKTFREDLYYRLIGLPIALPPLRERGNDILILAKHF
                     IDLFCKDNKIDKKTLSPEAQQKLLQYPFPGNVRELKSVVELASVMADEEMIQAEHITL
                     NTSASIADLLNREMTLKEFEIQILQHYLDKYDKDVLLVAKKLDVGKSTLYRMIQNGEL
                     KMK"
     gene            3372..5822
                     /locus_tag="VGQ51_02215"
     CDS             3372..5822
                     /locus_tag="VGQ51_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV9035399.1"
                     /translation="MHLENDFFEEILDKVPAEIIVADAEYRFLYVNPSAIPEPDLREW
                     MIGKTNEEFCQHSGRPVAMSRARRMVFEKARRTHNVVEWMESVQDSDGVVQHFQHRVF
                     PIYDEENTFRGVIVYAVRITETKEFEEKVKRSEKRYRDLFNHSQALICTHDLDGRLLS
                     VNPAICKVLGYEEQEMLGRSIKDFVPEKHQARFEEEYIKLIRDSTNAVSGEFCVLNKG
                     GEEIYMLYKNYRMEEPGSDPYVIGFSQDITERIRTEKELRLTKQLTDEMARAKESFLA
                     HMSHEIRTPMNGILGIASLLNKTELDPQQRNYLKLIQESAGNLLVIVNDILDLEKIVA
                     GKLQLEQIPFKIVDKIATIIQSFIYRAEEKELGLIFQNSVPADMVVEGDPYRLSQVLN
                     NILSNALKFTDAGHINIITGISERNEEEVVIEITIQDTGIGIGKEQLRTIFEPFEQAD
                     ATISRKYGGTGLGLAISKNMIEMQQGELLVDSEEGRGSAFTIRLPYLLSIEAMQENES
                     SQEIDYKSLGKRKVLVAEDVELNQFLARHILESWDFEVVIAANGREALEAMEHSFFDC
                     VLMDVQMPEMDGIEATQHIRRLSDPVKAAIPIIALTANALKGDSEKYIAAGMTDYLAK
                     PFDEARLFRVISRNLSRPEPSLSESPTHAIVADPTQPMTSAPSAHAKSPSANPTAAAP
                     NPQKEFFTKHLNNNHNMSVENSRLYDLSMVQSVSGGDEGFIRRMVALFIETVPQNVED
                     LKTAMQEENWEQVGKTAHKLKSTIDSMGIKSIRQEIRTVEANARQKESLQDIPMLVTN
                     IDSVIRECIGQLQTEVLK"
     gene            5967..6359
                     /locus_tag="VGQ51_02220"
     CDS             5967..6359
                     /locus_tag="VGQ51_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9035400.1"
                     /translation="MEFNQQPVIFVVEDNVLYQNLIAKELETLSTNLHFYTNGESAVS
                     DLGKHPTVIVLDYNLDGKMNGLDTLQRVRDIDPNVYVILFSSQKGLNTKEVFLQYGTF
                     DFLEKNSLSLRTLRQMVDCVTSGSKPLN"
     gene            complement(6518..7123)
                     /locus_tag="VGQ51_02225"
     CDS             complement(6518..7123)
                     /locus_tag="VGQ51_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035401.1"
                     /translation="MALVVIAVVVNVYSGFWPAFLPYLLAVILLFGYFFFGPLRLIQE
                     HMESGNMEAAEKVLNSVRFPNLLYKPIRSVYYTLKGNIAMMKQDFDGAEKNMKKGLDL
                     GMPMKEAEGASLLQMGMLAMQKNDIRQGESYIRQAIRKGLPDKENQAAAFLQLCSIMM
                     TKREFRAAKDFFRKAKALKPATPQIVDQIKQIEKYISRIPG"
BASE COUNT         1791 a         1834 c         1809 g         1697 t
ORIGIN      
        1 ccagatcgag atcggggttt cgccgggtct cttctttgac aaaatcgaac aatgcagtca
       61 gcacctgcat ctgccagatc cgatcaggac ttgaaacaat ggaggctagt atccccgtat
      121 cgcggataac gttttcgaaa agggcctgca gggtcgtatt tggcacgtcc ccgatgagtt
      181 tctccagcgc ccgggacgct tttttcagtc cttcgggtat ccccgtatcg aacagatccc
      241 gtgcaggtcg ctgcgccttt tcataaagca aacggcggat ggacgtcctt tgttcttcat
      301 tatattgttt atccgccacc tcgacggaaa gctttgcgat ctctatcgga gcgataccga
      361 agaaatggaa atgcaggatc tcgaacagca tttcatcccc gccataaggc gcgtcatgct
      421 cggcagccag gtaacgcagc aaaagaatga tctgctgcgc cagcgcaatc ccgaaaagat
      481 cgaggctgcg tttgctgtat acggggatat tcttcaattg aaggaagcga gccagttcct
      541 ctccatacct gttctcctta tagatgatgg caatgtgacc gggagatatc cctttggcca
      601 gcaactgctc cacgcgcagg gtgatacccg tcatttcctg tcttggactg aggtattcca
      661 gcagccgggg aggatagcgc agctgacgtg tccgcggatg gcttgccagc aggtgcttgg
      721 aaagtccggg tatctgccgg acaaggcgtt cctggttccg ttggatcagc gtcctcgaaa
      781 catcgaggat aggctgggta gaccggtaat tattggttaa gacgacagtc agcaggtcct
      841 tttgatactg ctgggcaaac tccagcatat tctccacatt ggctccctga aatctataaa
      901 tgctctgatc gtcatctcct accacgaata cattcggctg ctcccaatag ctgatcagca
      961 ggttgacgag cctgttctgg accccgctgg tatcctggta ttcgtcgaca aggatatatt
     1021 ggtaacgttc ctggtaagac gacaacaggg gcttgttctc cccgaaggca cggatgaccc
     1081 agttgatcat atcgtcgaaa tcataacggt tatgcacgcg catcagctcc tgatagcgat
     1141 cgaattcgtc tacggccgct ctcagcttct ccatcctctc ggtttcttcg gcgatcttgt
     1201 cggttcggac ctcgcctttc ttgaaggacg ccgtggccct tttggcgata tattcttccc
     1261 tgtccggcag cgaggccagg tattggtcga tcttctcctt aataaaggtc gggttccagc
     1321 cttcgcgttt catcgtggag aacagatttt ggagattgtt catctcgaaa tacacgtccc
     1381 cgcgataacg cttcagggga tggttcttcg gaaaccggtc gataagggcc ttgaagagct
     1441 gtattttttc cagatccgaa atgggatcca ggctggtctt ttcgaacagc gacagatttt
     1501 cctggatgat atcgttgcaa aaggaatgga aggtatggat atgcacctta taggcatccg
     1561 gaccgataaa agacaggagc ctgcggcgca tggcgatcgc tccggcctcg gtataggtaa
     1621 ggcaaaggat attgccgggg aaaacatccg tatcgaggag tatcttcccg atccgggctg
     1681 ccaggatctg tgtcttgccc gttccgggtc cggctataac cataacgggt ccctccgtat
     1741 tgtctacggc aagacgttgt tgttcattta attgctgata ttcttcctga aaacgccgtc
     1801 tcagctgatc ctgacttatg gacatggtcc aaagatagga attcagataa tcccctcatt
     1861 tttacctttt gcaccgccag tttagtcctt cttcgataaa aagttgaata ttgggttgga
     1921 aaatgtctta atatgggaat aaattcgcca attcaacgtt aaatcttatc ctatacaatc
     1981 gtagtttatg aaaaaccatc actcgtttaa ggtctttatc gtagaagatg atgtctggta
     2041 tggctcgatg ctccagcact acctcgctct caaccccgaa tacgaggtca aaagattcga
     2101 atctccccga gattttttcg ctcagctgca tgagaacccg gacgtggtaa cgctcgacta
     2161 ttcgctcccg gattgtgatg gggccgaagt tctcaaaaag atcaaggaac ataacccgga
     2221 tatccgggtc atcgtcattt cgggccagga agacgtcgct accgccatca acctcctcaa
     2281 gaacggcgcc ttcgactaca ttgtaaagga tgatgatacc aaggatcgtc tctggaacag
     2341 tatcctccac ctcagggaga taagcaatct gaaacaggaa gtggagaccc tgaagagcca
     2401 ggtcagccgc aaatacgatt tctcgcagat gatcatcggg aagagcgaac agatacagaa
     2461 agtgttctcg atgatcgaaa aagcttcgaa gaccaacatc accgtctcga tcaccggaga
     2521 aacgggttcc ggtaaagaaa tgattgccaa ggcaatacat tataattctg accggcacaa
     2581 gctaccctat gtcgccgtaa acgtggccgc cattccgcgg gatctcatag aaagcgagct
     2641 tttcggtcat gagaaaggag cattcaccgg cgccgtcacc cgccggatcg ggaaattcga
     2701 ggaggccgac aaaggcactc tcttcctcga tgagataggc gagttggata tcaacctgca
     2761 agccaagttg ttgcgtgtat tgcaggaacg tgagatcacc cgcattggcg ccaacggcat
     2821 caccaagatc gatgtgcgga tcgtcgtcgc cacccacaaa aatctgctgg atgaagtcaa
     2881 gaacaagacg tttcgcgagg acctatatta ccgtctgatc ggtctgccga tcgcgctgcc
     2941 gcccttacgt gaaaggggta atgacatcct gatcctcgcc aagcatttca tcgacttatt
     3001 ctgtaaggat aacaagatcg ataaaaagac gctttctccc gaagcccagc agaagctgtt
     3061 gcagtatcct ttcccgggta atgtgcgcga gctgaaatcg gtggtggagc tggcttcggt
     3121 catggccgat gaagaaatga tacaggccga gcatattacc ctgaatactt cggccagcat
     3181 tgccgatctg ttgaacaggg aaatgaccct taaagagttt gagatccaga tcctgcagca
     3241 ttatctcgat aaatatgaca aggatgttct gctcgtcgcc aaaaagctcg acgtgggtaa
     3301 atccacgttg tatcggatga tacagaacgg tgagctgaag atgaaatgat gcaagatcaa
     3361 aaaccctggg tatgcactta gagaatgact tttttgagga gatcctcgat aaggtccctg
     3421 cggagatcat cgtggcggat gcggaatacc gtttcctcta cgtgaatccc agtgccatac
     3481 cggaacccga tctcagggag tggatgatcg gtaagaccaa tgaagaattc tgccagcatt
     3541 cagggcgtcc tgtcgccatg tcacgggccc gtcggatggt ctttgaaaag gcgcgtcgga
     3601 cccacaatgt agtggaatgg atggaatcgg tccaggacag cgatggcgtc gtacagcatt
     3661 ttcagcaccg cgtttttcct atttatgatg aagagaacac cttccgtgga gtgatcgtct
     3721 atgccgtccg gatcacggag actaaagagt tcgaggagaa ggtgaaacgc agcgaaaaac
     3781 gatatcgcga tctattcaac catagtcagg cactgatctg cacccatgac ctcgatgggc
     3841 ggctgctgtc cgtgaacccg gccatctgca aagtattggg atatgaagaa caggaaatgc
     3901 tgggccgaag catcaaggat tttgttcccg agaaacatca ggcgcggttc gaagaggaat
     3961 atatcaagtt gatccgggac tccaccaatg ccgtcagcgg cgagttctgt gtgctcaaca
     4021 aggggggcga ggagatctat atgctgtata agaactaccg gatggaagag cccggttcgg
     4081 acccctatgt cattggattt tcgcaggaca tcaccgagcg gatccggacg gaaaaggagc
     4141 tccgtcttac caaacagctg accgacgaga tggcccgggc gaaagaatcc tttttggcgc
     4201 acatgagcca tgagatccgg acgcccatga atggtatcct cgggattgcc agtctgctga
     4261 acaaaacgga gctcgacccc cagcaacgaa attatctgaa gctgatccag gagtcggcgg
     4321 gcaacctgct ggtcatcgtc aatgacatcc tcgacctgga aaagatagtt gcagggaaat
     4381 tgcagttgga gcagatcccc ttcaagatcg tcgacaagat cgccacgatc atacagtctt
     4441 ttatataccg cgcggaagag aaggagctgg gtctcatttt tcagaactct gtccccgcag
     4501 atatggtagt agagggcgac ccttatcgtc tcagccaggt actgaacaat attctcagca
     4561 acgcgttgaa gtttacggat gccgggcata tcaacatcat caccggcatc agtgagcgta
     4621 atgaggaaga ggtggttatc gagatcacca tccaggacac ggggatcggc atcgggaaag
     4681 agcaattgcg taccatattc gagcctttcg aacaggccga cgcgacgatc tcgcggaaat
     4741 atggaggcac gggactcgga ctcgccatct cgaagaatat gatcgagatg cagcagggcg
     4801 agcttcttgt cgacagtgag gaaggcaggg gatcggcctt taccattcgg ctaccttatc
     4861 ttttgagtat tgaagctatg caggaaaatg agtccagtca ggaaatcgac tataaaagcc
     4921 ttggcaagag aaaagtattg gtagcggagg atgtggaatt gaatcaattc ctggcccggc
     4981 atatcttgga gtcctgggac tttgaagtcg tcatagcggc caacgggcgg gaggccctgg
     5041 aggcaatgga gcattcattt ttcgattgtg ttctgatgga tgtgcagatg cccgagatgg
     5101 atgggatcga agcgacacag catattcggc gtctttcgga tcccgtcaag gctgcgattc
     5161 ccattattgc gctgacggcc aatgcgctga agggcgatag tgagaagtat atagcggcgg
     5221 ggatgaccga ttatctggca aagccgttcg atgaggcgag gttgttccgg gttatttcaa
     5281 ggaatctttc gcggccggag ccttcccttt cggaatcccc aacgcatgcg atcgtcgccg
     5341 accccacgca accgatgacc tcagcccctt cagctcacgc gaaatccccc tcagcgaatc
     5401 cgacggccgc tgcccccaac ccccaaaaag aattttttac caagcacctt aacaacaacc
     5461 acaatatgtc agttgagaac tcaaggcttt atgatctttc catggttcag tccgtatcgg
     5521 gcggcgacga aggctttatc cggcggatgg tggccctttt tatcgaaacg gttccccaaa
     5581 acgtagagga cctcaaaacg gccatgcagg aagagaactg ggagcaggtg ggcaaaacgg
     5641 ctcataaact aaagtcgacc atcgactcca tgggaataaa gtccattcgt caggagatcc
     5701 ggacggtcga ggccaatgcc agacaaaaag aatctctcca ggatatccct atgctggtaa
     5761 ccaacatcga ctcggtgatc cgtgaatgta tcggccagct gcagaccgag gtgctaaaat
     5821 gattcccacg tacaggtcaa ttttcccaaa cagggattag ggtttttact agtggcaacc
     5881 attctgaatg acaatcaaca aattacatca attttttttc tggcattatt ttccattgac
     5941 ttaccctcaa tacagccaca aagccaatgg aattcaatca acaacccgtt atattcgtag
     6001 tagaggacaa tgtcctttac cagaacctta tagcgaagga gcttgaaacg ctcagcacga
     6061 acctgcattt ctataccaac ggggaatccg ctgtttccga tctcggcaaa catcctaccg
     6121 ttatcgtcct ggattataac ctggatggga aaatgaatgg tttggatacc ctgcaacggg
     6181 tccgggacat cgaccccaac gtctacgtga tcttgttctc ttctcagaag ggtctgaata
     6241 ccaaggaagt attcctgcaa tatgggacct tcgattttct cgaaaaaaat agtctttcgt
     6301 tacgtacgct gcgtcagatg gtggattgcg tcacctccgg cagcaagccc ttgaattaga
     6361 tcgtacccct ccctgacttc tcctcctgat cgctttttat ccctttctaa ctgttctccc
     6421 ttccgccgcc gctcaccccg gtttcgaatc actatgtccg ccggcttacc ccgacggccg
     6481 ccccggctca ccctgggaat ccgcatcatg acccgcatca ccccgggatt cgcgaaatat
     6541 acttctcgat ttgtttgatc tgatcgacga tctgcggcgt ggcaggtttc aatgcctttg
     6601 ccttccggaa gaaatccttg gccgcgcgga actcccgttt ggtcatcatg atcgagcata
     6661 gctgcagaaa tgccgccgcc tggttctcct tatcaggcag tcctttgcgg atggcttgcc
     6721 ggatatagct ttctccctgc cggatatcgt tcttttgcat cgccagcatt cccatctgca
     6781 gcaagctggc tccttcggcc tccttcattg gcatccccag atcgagcccc ttcttcatgt
     6841 tcttttccgc cccgtcaaaa tcctgtttca tcatcgcgat attgcccttg agggtataat
     6901 agaccgaccg gatcggctta tacagcagat tggggaaacg gaccgaattc aatactttct
     6961 ccgccgcctc catattgccg gattccatat gttcctggat caggcgcagc ggcccgaaaa
     7021 agaaatatcc gaagaggagg atcaccgcca gcagataggg gaggaaggcc ggccagaacc
     7081 ccgaatagac atttacgacc accgcaatga ccactaaagc gatgactaat t
//
LOCUS       DASYSV010000072         8516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2448940, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000072 DASYSV010000000
VERSION     DASYSV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2448940"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VGQ51_02230"
     CDS             <1..989
                     /locus_tag="VGQ51_02230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEV9035402.1"
                     /translation="VGYIVSCSARNIVNNVLDMAGIETGKTPRIVSGPIAVIPFFREV
                     MIVHKTIAANEDKRLRLEIDMPKVIFGDPLVITQILTNLLTNALKYGARGTIIMVRVK
                     RAGVNWEIAVSNYGEVVFTDLNEIFDPFVTGKTGQIQGSGLGLFIVKNKVSQMGGTIR
                     AESHPGGYTTFTVALPLVEGKPSDLPEEGNPNVEVIDLSSIRVLVAEDNNLTAFLLTT
                     FLEDLGCQFEIVSNGRDLLELAQKKSPDEYPDIILLDSRMPVLGGEETIRMLKRLPVL
                     NEIPIIVSTADVYSDALERMLKAGASTYLKKPIDHQALKRAISLYVKKISLE"
     gene            complement(1046..2155)
                     /locus_tag="VGQ51_02235"
     CDS             complement(1046..2155)
                     /locus_tag="VGQ51_02235"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010333 - terpene synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terpene synthase family protein"
                     /protein_id="HEV9035403.1"
                     /translation="MKKVLPLKAAVSSILQRTQTEYRALVQTESWSLKELFSPAHFRF
                     DEFCRGFSPHPDSTRLTQLAQQFGEEHDIWLPNAKGHVSCALFLYPSAHAGRMIAIMK
                     NLIMDFYLNDVMGRDLFKFLSVNQQRDARRLIASMAGIRENLVVSPDAHPIEHLNAQV
                     LREFRENSPEPWFRKFFNFYCHHLNITHSDNDSEALGYIPEVDEYIDKRCHYAGMYHI
                     VLWVEYNNARFLDWDLLGDLGLASPLRRLHYLTTAFGALSNDLFSFEKEVIDQGSDCN
                     LVMVAMLNNPQWSLKEGIAASCRIVRDMVHELSGILRSVRRRIDILERTDPGLSSALT
                     DHIEGVLRCIQASWLWQCHSTRYKRAQSLWEETTL"
     gene            2349..2960
                     /locus_tag="VGQ51_02240"
     CDS             2349..2960
                     /locus_tag="VGQ51_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9035404.1"
                     /translation="MQPVILLADDHSMIRKGLRSSFRFDLGYSNITEVTSCTELMSQL
                     KKKIFTHLILDINLSDGSSLEILPNIRRLYPHLQIAVLTMQPAAIFRNVLKQYGIYHF
                     MQKTAPEEDTTRMLAQFLQNEMPARHASDNDAPSNPFLALSSREHEVLHYLLKGTSTT
                     EIGEKLNLKQNTISTFKTRIFEKTGTRNLQELAELATAYKIKG"
     gene            3121..3606
                     /locus_tag="VGQ51_02245"
     CDS             3121..3606
                     /locus_tag="VGQ51_02245"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEV9035405.1"
                     /translation="MDLINTFFGHGKDLNSLQMICRSIVAFFLTLVMIRVAGIRTFGK
                     KTPFDNVIIIMLGSIFSRVVVGVSPFIATTLACFVFVLIHRLLALISFYNDTVGRWIK
                     GEASTLYADGQPVRRNMRSANVSDKDLMESVRRQMHEDSLANVSEIIQERNGEVSVVK
                     K"
     gene            complement(3639..4301)
                     /locus_tag="VGQ51_02250"
     CDS             complement(3639..4301)
                     /locus_tag="VGQ51_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEV9035406.1"
                     /translation="MAQTRWLEFYTQQFDATEINASFYRLPSKTTVEAWQKRVGRHFY
                     FCPKISRFITHAKKLNDPEQSVPRFFDVFGLLKRQLGPVLIQLPAALAYHEEKATIFF
                     GFLRKHYPDFSYALEARHLSWTQPQAEALLKRYDIGWVIADSGGRFASARLVTARHIY
                     LRFHGPDGSYATPYTRRQLAAYAAKCRRWREEGYSVWAFFNNDVHGHAPRDARTLKEL
                     TA"
     gene            4518..5015
                     /locus_tag="VGQ51_02255"
     CDS             4518..5015
                     /locus_tag="VGQ51_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEV9035407.1"
                     /translation="MSYCVDVVIETPKGSSQKYDYVPNTPFFKLKKILPSGMVFPYDF
                     GFIPNTRGEDGDPLDVIVVSEFNSFPGCIIKCRIIGGIKAEQSESKDSHKMIRNDRFL
                     AIPKFTNIFQNVKKMDDLPGQVTDQLEEFFVDYNKMQGKKFKALSKMGSGEAHRMIEE
                     LLQPA"
     gene            complement(5052..5738)
                     /locus_tag="VGQ51_02260"
     CDS             complement(5052..5738)
                     /locus_tag="VGQ51_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEV9035408.1"
                     /translation="MQTLRFNPFNQIHKGLRALLYDTALLLQHTDFTRAEEKERITDR
                     MHFLIEAFAFHAHTEDHRIFPMITGFAPELVDDFESQHQTDEQLSLGLQECVNTLAEA
                     NSPSQNVWAGNELLQAFNSFLAFNVEHMKKEETMINAVLWQHYNDEQLLFRVREIGGS
                     IPIEENMLISAWMLKGLAVHEIIPWYKGIEKAAPPVVFEKFRQLAERTLAPSRWSKIR
                     EALQDTEQVL"
     gene            5839..7773
                     /locus_tag="VGQ51_02265"
     CDS             5839..7773
                     /locus_tag="VGQ51_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9035409.1"
                     /translation="MKTSAFLLAMMICLSVSAQDKHVSDSLLTILRSGRQDTVMVSAL
                     LSWGDRYSIRMPDSAVFYFEKAHRLAEGLHWSRGIAGYDSHIIKILDDRGDYQEALRL
                     CKEAIRIYSDSGTREDMVRGYNNLANEYQYLGDLDATVDNYMNAIRLAETLTQRRYMK
                     ILTNNLASVFYELGQFDKCYTYALQAYHIAGELKDTEGIASSLVNLGLSEGHLDRTDS
                     AMKHFREIVRFGRRLDDYTLLCDAYVNIASIYVRQHRAVLAKPKLDSVLLLADKHDNP
                     NYRMLALAGLADVAAEDHEWQEADRYTKDAMAIASLLRDRDDLVQLFDKEATIREKML
                     DWKGALAFRNRFDTLNDSLLNEKTRNHINDLETRYQAARKDHALAEQRLQLAQTKEIV
                     RRRNTQLIILTGGVTALVILLLLAWRLFRQRQKLNRQALITLQKEQEVTVLKAVLEGQ
                     EDERQRIARELHDDMGAGLTTILYLSRQPAADGATATGEKIARTAAGLVDTINEIIWS
                     MNQRYDSLEDLVTFIRRQASQLLEINGIDYRIEIPERVPDIRLRGELRRNVYLVVKEA
                     INNALKHAGASLILLNIDIDSKLHIRVQDNGRGLGADRNRKNRVGNGLGNMQQRMEKA
                     GGTICIVSDRGTIVRLTVPI"
     gene            7819..8448
                     /locus_tag="VGQ51_02270"
     CDS             7819..8448
                     /locus_tag="VGQ51_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9035410.1"
                     /translation="MTSIGVSIIEDIADIRHGLERIIADAPDFLLLSSYDNAEDAISS
                     LPDIRPDIVIADIQLPGLSGIDCIRLVKAVCPSVQFMMFTIYEDSEQVFEALEAGANG
                     YLLKQTPQDRILDAIRELHQGGSPMSSGIARRVVSSFRKQALQSADANVLSARESEVL
                     QLLAKGLLYKEIGQRLQISTGTVRQHIHKIYGKLQVQNKTEAINKAFRK"
BASE COUNT         2081 a         2279 c         2247 g         1909 t
ORIGIN      
        1 atgtgggtta tatcgtgagt tgctctgcgc gcaatatcgt gaacaacgtg ttggatatgg
       61 ccgggataga gaccggaaag actccgagga tcgtgagcgg gcccatcgcg gtcattccct
      121 ttttcaggga ggtcatgatc gtacacaaga ctatcgccgc caacgaggac aaacggctgc
      181 gtctcgagat cgatatgccg aaagtgatct tcggcgaccc gcttgtgatc acccagatat
      241 tgaccaacct gctgaccaat gccctcaaat acggtgcgag aggtaccatt atcatggtgc
      301 gcgtcaagcg tgccggcgtc aattgggaga tcgccgtgtc caactacgga gaggtagtgt
      361 tcaccgacct aaacgagata ttcgatccct tcgtgacggg aaagaccggt cagatacagg
      421 gctcgggact gggtttgttc atcgtaaaaa ataaagtgag ccagatgggt ggtacgatcc
      481 gggcggaaag tcacccgggg ggatatacca cttttaccgt tgccctgccg ctggtcgaag
      541 gcaaaccgtc cgatctgccc gaagaaggga atcccaatgt cgaggtcatc gatctcagtt
      601 cgatccgcgt attggtggcc gaggacaata atctcacggc cttcctgctg accaccttcc
      661 tggaggacct gggatgccag ttcgagatag tcagcaacgg acgcgatctg ctcgagctgg
      721 cgcaaaaaaa atctccggac gagtacccgg acatcatcct gctggattcc cggatgccgg
      781 tgctcggcgg cgaggaaacg atccggatgc tcaaacgact acccgttctg aacgagatac
      841 cgatcatcgt ctccaccgcc gatgtctatt cggacgcgct ggaacgcatg ctcaaggcag
      901 gcgccagcac ctatctgaaa aaaccgatcg atcaccaggc attgaaaagg gcgatcagcc
      961 tctatgtaaa aaaaatctcg ctggaatgac cgcgatcgtt cagcgcgacc ccggtttccg
     1021 acccggtgaa tgcgacctgc cctccttaca gcgtcgtctc ctcccacagc gactgcgcgc
     1081 gtttgtagcg ggtggaatgg cactgccaaa gccagctggc ctgtatgcaa cgaaggacgc
     1141 cttcgatatg atcggtaagc gccgacgaca accccggatc ggttcgctca aggatgtcga
     1201 tccgcctgcg caccgatcgc aagataccgg agagctcatg caccatatcc cgtactatcc
     1261 tgcaggaggc cgcaatgcct tccttcagcg accactgtgg gttattgagc atcgcgacca
     1321 tgacgagatt gcaatcgctg ccctggtcga tcacttcctt ttcaaaggaa aaaagatcgt
     1381 tcgacagcgc tccgaaggcg gtggtcaggt aatgcagccg ccgcaacggt gacgccagac
     1441 ccaggtcgcc gagcagatcc cagtcgagga accgggcgtt gttgtattcg acccagagga
     1501 cgatatggta cataccggca taatgacaac gcttatcgat gtattcgtcg acttccggta
     1561 tgtatcccag cgcttccgag tcgttgtcgg agtgggtgat gttcaggtga tgacaataga
     1621 aattaaaaaa cttccgaaac cacggttcgg gggagttctc cctgaattca cgaaggacct
     1681 gcgcgttgag atgctcgatg ggatgggcgt cgggtgacac gacaaggttc tcacggatac
     1741 ccgccatact ggcgatcaac cgcctggcgt cgcgttgctg gttgacggaa aggaatttga
     1801 acagatcgcg acccatgacg tcgttgagat agaaatccat gatgaggttc ttcatgatag
     1861 cgatcatccg ccccgcatgc gcggaaggat acaggaacag cgcacacgag acatgtcctt
     1921 tggcgttggg cagccagata tcgtgttctt cgccaaactg ctgagccagt tgtgtcagcc
     1981 tggtactgtc gggatgcgga ctgaatcccc ggcagaattc atcgaaccgg aaatgcgctg
     2041 gggagaacaa ttctttgagc gaccaggatt cggtttgaac aagcgcgcga tactccgtct
     2101 gggtgcgttg aaggatggag gacaccgccg ccttaagggg aagtactttt ttcataaaat
     2161 aagctttaaa tggttaaaaa atcgatgtat caaaatcaaa tacggggccg taatcgcagt
     2221 gttttatcat cttcataaat taagtagatc accttataaa actgcgacat gaatgcagtc
     2281 tgcggaaaaa ccaacatagg ggtttccggg tattttacgt atcattacaa acctccaaat
     2341 acgacggcat gcaacccgta attcttttgg cggatgatca cagcatgatc cggaagggat
     2401 tgagatcctc attccggttc gatctgggat actccaatat caccgaggtc accagttgta
     2461 ccgagctgat gagccagctg aaaaaaaaga tatttactca cctgatactt gacatcaatc
     2521 tttccgacgg cagcagtctc gagatcctgc ccaatatccg tcgtctgtac ccgcatcttc
     2581 agatagcggt gctgaccatg cagcccgccg ccatcttccg caatgtgctg aaacaatatg
     2641 gcatctatca ttttatgcaa aagaccgcgc ccgaagaaga tacgacgcga atgctcgccc
     2701 aattccttca gaacgagatg cccgcgcgtc acgcttccga caacgatgcc ccttccaatc
     2761 cattccttgc gttatccagc cgtgagcatg aggtgctcca ctatctcctc aaaggcacca
     2821 gcacgaccga gatcggtgag aagctcaatc ttaaacagaa taccatctct actttcaaga
     2881 cgcgcatctt cgagaagacc ggcacgcgca atctccagga gctggcagag ctcgccaccg
     2941 cctataagat caaaggctga ggtccttgcc gaaggcacga tattttcagt ggtaaaatta
     3001 ttcctccgac aatggtcatg gcgtcgtccg ggaccatggg gaagggtcct ccttctcttt
     3061 tgtgcttcct gttggtgaca ctgggccggt ttttgccaca ggatcttaaa agcattcctt
     3121 atggatctca ttaatacctt ctttggccat ggtaaagatc tgaacagtct ccagatgatc
     3181 tgccggtcga tcgtcgcctt tttcctgacg ctggtcatga tccgtgtggc gggcatccgc
     3241 accttcggga aaaaaacacc cttcgacaat gtgatcatca tcatgctcgg gtccatcttc
     3301 agccgcgtcg tagtcggcgt ctcccctttt atagcaacga cgctggcctg cttcgttttc
     3361 gtactgatcc atcgactgct ggccctgatc agtttctaca acgacacggt cggccgctgg
     3421 atcaaaggtg aggcttcgac cctgtatgcg gacggtcagc ctgtccggag gaatatgcgc
     3481 tccgccaatg ttagcgacaa ggaccttatg gaaagtgtcc gacggcagat gcatgaggat
     3541 agtctcgcca atgtcagcga gatcatccag gagcgcaacg gagaagtgag tgtggtgaaa
     3601 aaatagggaa tgcacacctc atggcatgga ttttttgatc acgctgtcag ctctttcagg
     3661 gtgcgggcat cccggggagc atggccatgg acatcgttat taaagaatgc ccagacggag
     3721 tatccttcct cacgccatct cctgcatttt gcggcatatg ctgccagctg ccgtcgtgta
     3781 tagggggtgg catagcttcc gtccggtccg tgaaaccgca gatagatatg ccgggcagtc
     3841 accagcctgg ccgatgcgaa gcgtccgcct gaatcggcga tcacccatcc gatgtcatag
     3901 cgtttcagca atgcctccgc ctgcggttgc gtccatgaca ggtgccgggc ctccagagca
     3961 tagctgaaat ccgggtaatg ttttcggaga aacccgaaga agatcgttgc cttttcttcg
     4021 tgataagcca gcgcggccgg caactggatc agtaccggcc ccagttgtcg tttcaataac
     4081 ccgaaaacat cgaagaaccg ggggacgctc tgctcagggt cgtttagctt ttttgcatgg
     4141 gtgatgaacc ggctgatctt cggacaaaaa tagaaatgcc gccctaccct cttttgccag
     4201 gcctcgaccg tcgtcttcga cggcagccgg taaaagctcg cattgatctc ggtggcgtcg
     4261 aattgctgcg tatagaactc gagccacctg gtttgcgcca tccctaccgg gtaaaaagat
     4321 tctctccagt ggggatatac ccaccccgaa gtgccgatat gaatagctgc aggcatcgga
     4381 taagagcccc gaaagatcat gccggcattc cagcccggac cgcctccgcc tgataccccg
     4441 gggatccgaa aggcataaag attgcaaccg tggctttgac acccattctt ttacaactgt
     4501 tataaatcga attaattatg tcctattgtg ttgatgtagt gatagaaacc ccgaagggca
     4561 gctcgcagaa atacgattat gtgccgaaca cgcctttttt caagctgaag aagatcctcc
     4621 cttccggcat ggtctttcct tacgacttcg gatttatccc gaatacccgc ggcgaagacg
     4681 gcgatccgct ggatgtgatc gtggtctcgg aattcaacag ctttcccggc tgcattatca
     4741 aatgccgcat cattggcggg atcaaggccg agcagtccga gagtaaggac agtcacaaaa
     4801 tgatccgtaa cgaccggttc ctggccattc ccaaattcac caacatcttc cagaatgtca
     4861 aaaaaatgga tgacctgccc gggcaggtga cggaccagct ggaagaattt tttgtggatt
     4921 ataacaaaat gcaagggaaa aaattcaagg cgctgtcaaa gatgggttcc ggcgaagcgc
     4981 atcgtatgat cgaagagctt ttgcaacccg catgacagcc ataatgcttc tgccagccgg
     5041 cgctactctt cttataatac ctgttccgta tcctgtagcg cttccctaat cttgctccac
     5101 ctcgacggtg ccagtgttct ttcggcaagc tgacggaatt tttcaaaaac aacaggcgga
     5161 gcggcctttt cgatcccttt ataccaggga atgatctcat ggaccgccag gcctttcagc
     5221 atccacgcgg agatcagcat attttcttcg atcggtatcg agccgccgat ctcccgcact
     5281 ctgaaaagca gctgttcgtc attgtagtgt tgccaaagca cggcattgat catggtctcc
     5341 tccttcttca tgtgttctac attgaatgcg agaaaggaat tgaatgcctg gagcaattca
     5401 ttgccggccc atacattttg agagggagaa ttggcttcgg caagcgtgtt cacacactcc
     5461 tgcaggccta gcgacagttg ttcatcggtc tggtgctggc tttcgaaatc atcgaccaat
     5521 tccggcgcaa atccggtgat catgggaaag atccgatggt cttccgtgtg agcatgaaaa
     5581 gcaaatgcct cgattaaaaa atgcatccga tcggtaattc tctctttttc ttccgcccgg
     5641 gtaaagtcgg tgtgctgaag taacaatgcc gtatcataca gtaatgcgcg gagacccttg
     5701 tgaatttgat tgaatgggtt gaatcgtaac gtttgcatag ataaatttta ttgcaaagta
     5761 caaacctgag taacggccgg gaatataacc ttcgttatat ccgccggctt tcctatcttt
     5821 agagacctga catcgaccat gaagacctcc gctttcttgc tggctatgat gatttgcctg
     5881 tctgtatctg cccaggacaa acatgtttcc gacagtcttt tgaccattct gcgatcaggc
     5941 agacaggata ccgttatggt cagcgcatta ctctcgtggg gagaccgcta tagcatccgg
     6001 atgccggatt cagccgtgtt ctattttgaa aaagcccatc gacttgccga aggattgcac
     6061 tggtccaggg ggattgccgg ctatgacagt catatcatta aaattctgga cgaccgcggc
     6121 gattaccagg aggcactcag gctttgcaag gaagccattc gtatctacag tgacagcggg
     6181 acgcgcgaag atatggtccg gggttacaac aatctcgcca atgagtacca gtatctcggc
     6241 gatctggacg ccaccgtgga taactatatg aatgcgatcc ggctggcgga gacgctgacc
     6301 caacgacggt acatgaagat cctcacgaac aatctggcgt ctgtgttcta tgagctgggg
     6361 caattcgaca aatgctatac ctatgccctg caggcctatc atattgcggg tgagctgaaa
     6421 gatacggaag gcatcgccag tagtctcgtc aacctcggtc taagcgaggg tcatctggat
     6481 cgaacggact cggctatgaa acatttccgg gagatcgtgc ggttcggccg ccggctggat
     6541 gactataccc tactctgcga tgcctacgtg aatatcgcaa gcatctatgt ccgacaacac
     6601 cgcgcggtcc tcgcgaaacc caaattagac agcgtcctgc tcctcgcgga taagcatgac
     6661 aatcccaact accggatgct ggcgctggca gggctggcgg atgtcgccgc cgaagaccat
     6721 gaatggcaag aggccgatcg gtatacgaaa gatgcgatgg cgatagcctc gttgctccgg
     6781 gaccgcgatg atctcgtaca gctgttcgac aaagaagcca ctatccggga aaaaatgctc
     6841 gattggaaag gagcgctggc tttccgtaac cggttcgata cgctgaacga ctcgctgctc
     6901 aatgaaaaga cgcgcaatca tatcaacgac ctggagacgc gttaccaggc ggccaggaaa
     6961 gatcatgcac tggcagagca gcgtctgcag ctggcgcaga caaaggagat cgttcgcaga
     7021 cggaataccc agcttatcat cctgacgggc ggggtgacag ccctggttat cttgttgttg
     7081 ttagcatggc ggctattccg ccaacgtcag aaactgaacc ggcaggcgtt gatcaccttg
     7141 caaaaagagc aggaggtaac ggtgctgaag gccgtgctgg aaggtcagga agacgagcga
     7201 cagcgtattg cgagggaact gcacgatgac atgggtgcgg gtctcacgac gatcctttat
     7261 ctgagccggc aacctgctgc agacggcgcg accgccaccg gcgaaaagat cgcccgcacc
     7321 gcggccggtc tcgtcgatac gatcaacgag atcatctggt ccatgaacca acgatatgat
     7381 tcactggaag atctcgtgac attcatccgc cggcaggcca gccagctact cgagatcaat
     7441 ggcatcgact accgtatcga aatccccgaa cgggtccccg acatccgtct tcggggtgag
     7501 ctgcggcgaa acgtttacct cgtggtaaag gaggcgatca ataatgcact gaaacacgcg
     7561 ggtgccagcc tcatcttgct gaatatcgac atcgattcga aacttcatat ccgggtacag
     7621 gacaatggca ggggtctggg ggccgacagg aaccgtaaaa atcgggtcgg caatggcctg
     7681 ggcaatatgc agcaacggat ggagaaggcg ggcggtacca tctgtatcgt ttccgacaga
     7741 ggtaccatcg tccggctaac cgtcccgata tgaccttcgt tatattggcg atccggccgg
     7801 aagccgtatt tttatgccat gacatccatt ggcgtcagca ttatcgaaga tattgccgat
     7861 atacgacacg gcctggagcg gatcatcgcc gacgctcccg acttccttct cctttcctcc
     7921 tatgacaacg cggaagacgc cataagctcg ctgccggata tccgtccgga catcgtgatc
     7981 gccgatattc agctgccggg attgagcggc atcgactgta tccgtctcgt aaaagccgtc
     8041 tgtccttccg tccagttcat gatgttcacc atctatgaag acagcgaaca ggtcttcgaa
     8101 gcgttggaag cgggcgccaa cggctatctc ctcaaacaaa cgcctcagga ccgcatcctc
     8161 gatgcgatca gggaactgca tcagggcgga tcaccgatga gctcgggcat cgcgcgacgg
     8221 gtcgtcagct cattccgtaa gcaggctcta caatcggccg atgcaaacgt gctttcggca
     8281 cgcgaaagcg aggtcctgca acttctcgca aaaggtctgt tgtacaagga gatcggccag
     8341 cgtctgcaga tcagtacggg aaccgtgcgc cagcacatcc acaagatcta tggtaaactc
     8401 caggttcaaa acaaaaccga agccatcaat aaggcgttca gaaaatagca ggtatgacga
     8461 acgttctatt tgggcgaaaa tgatcgggtt ctaacttgcg ggaccaacca acgatc
//
LOCUS       DASYSV010000073         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2193038, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000073 DASYSV010000000
VERSION     DASYSV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193038"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..3162
                     /locus_tag="VGQ51_02275"
     CDS             154..3162
                     /locus_tag="VGQ51_02275"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEV9035411.1"
                     /translation="MMPYILHVTVITTICFLFYKLLLQKETFYHLNRWILIGCLAVSF
                     GLPLLPVPREFSWRDKWTELTNRQDALQIASRTNAETKFVRNTTDVGSPVIDASSTDA
                     SIGSLVQADPKGLPADAKAKTKAGHKPRHGEHSRMMSDKATKAEAGMAGILNGVEPNV
                     AAAARGQKTEAAAQGQKEEAAAQGQKTEAAAQGQKPEAASQGKDTNVAGTPSTPIFST
                     ANLFLVLTWLFYGYLFGVLVFGGSFLMQIAVLLYQSYSRPVIRDGRFRIVEVRGNRAP
                     CSFGNTIFINPENYDWETYNQILIHEKIHVSGRHTLDILLAEIALVLQWFNPFVWWYR
                     REVENNLEFLTDASVLLHRDVERSAYQLSLLRVSAPHLPFSITNNYNQSLLKRRIVMM
                     NSKRSSLHTVWKYFFLLPVLSVLMCALNKPAALAATVRSSAPGHSGTTANQDGPALTA
                     NPLKLLAAKVASAFGDTTIRPASGNASSSEGSGNSDSSPDHHYYNEDDYQMLKAYSAG
                     QNAYDLAHLNFKLAAATQASLQLLFKNPQIHLDEQLVQAEKIQLKMLKDNQVHFKFDP
                     SINMNMDIKAKIFSKIDGSHFDLSHMDMMADTGLREGAWFATLSDEKDKDKMRIELRS
                     GDDNHNWNSTFSVDKKDINPFPGQGNVEFKLVREAGTISFKGQFDGQEGFGHFKYTPD
                     EGYLAAIKQMGVEDVDDRRQFAYFTLDIKKDYVNMLTHSGYPHISQHDLISFAAMKID
                     KAFIDNWRGSGFEDADNPRNLISLKAMNIDRAYVDEMKSAGYDHLSIHELMSLRAMKV
                     DRAYIRSLGRNSDNTPIPVHEVISYKAMNIDSGYVEGLRKAGYTNLTRHDVTSLYAMH
                     VTPEFIRGLQDAGYTNIPVHELTSLKAMNVTPEFIKSYREMGFKDLNVRELTSLKAMN
                     ITPEFIKSFRDIGYTDLSARELSSLKAMNVTPEFVNGFKKLGFDHIPVHTLTSLKATG
                     VDADYVAKMKAKGFVSEDLNKYIRLKNDFN"
     gene            3202..>3549
                     /locus_tag="VGQ51_02280"
     CDS             3202..>3549
                     /locus_tag="VGQ51_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9035412.1"
                     /translation="MAGRQSDPARIRIMVTIFFFVSGFGFSTWASRIPTLQQQLHLNE
                     AQLGAVLFALPIGLMGTLPLTSYLLSRYDSRQVMMIGAVLFNVLLCCIGLAAHPWQLA
                     ILLVCFGSSRNLMN"
BASE COUNT          865 a          946 c          959 g          779 t
ORIGIN      
        1 gaaagaacaa taccagagcc atgctttcga cgatatcgtc aagcaatatt tcaataactc
       61 ctatccgcgg atgctggcct acttcgccaa agaacaaaag atatcgcggg aagaactaaa
      121 cgaaatcctg gagcttattc aatcccaaaa gaaatgatgc cttatatcct gcatgtaacg
      181 gtgatcacca ccatctgttt cctgttctat aagctcctgc tgcagaagga gactttttat
      241 cacctgaaca gatggatcct gataggttgc ctggcagttt ccttcggttt gcctttgctg
      301 cccgttccgc gcgagttcag ctggcgcgat aaatggacgg agctgacgaa ccggcaggat
      361 gcgctgcaga tagcctcccg gacaaacgcc gaaacgaaat tcgtgcggaa cactaccgat
      421 gttgggtcgc cggtgatcga tgcatcctcc accgatgcgt caatcggctc cctggtgcaa
      481 gcggatccga aaggactgcc cgcggatgcg aaagcgaaga cgaaggccgg gcacaagccg
      541 cgacacggcg aacatagcag gatgatgtcg gataaagcaa cgaaagcgga ggccggcatg
      601 gcgggcattt taaacggagt cgagcccaat gtcgcggctg cggcacgggg tcaaaagacg
      661 gaggccgcgg cacagggcca gaaggaggag gctgcggcac agggtcaaaa gacggaggcc
      721 gcggcacagg gccagaagcc ggaggccgca tcacagggaa aagatacgaa cgttgcgggg
      781 acgccttcga cgcccatctt ttccaccgcc aaccttttcc ttgttctcac ctggcttttt
      841 tacggctacc tgttcggcgt cctagttttc gggggaagct ttttgatgca gatagccgtc
      901 ctgctgtacc agtcctattc acgcccggtg atccgggacg gtcgtttccg gatcgttgag
      961 gtccggggca accgcgcacc ctgctcattc ggcaacacca tttttatcaa cccggaaaac
     1021 tatgactggg aaacctacaa ccagatcctt atccatgaaa agatccatgt cagcggccgg
     1081 catacgctgg atatcctgct ggcggagatc gccctcgttc tccaatggtt caaccccttc
     1141 gtatggtggt atcgcaggga agtggagaac aatctcgaat tcctcaccga cgcatcggtg
     1201 ttgctgcacc gggacgtaga gcggtcggcc taccagctga gtttgctcag ggtatctgcc
     1261 cctcatctgc ctttcagcat taccaacaat tacaatcaat cactccttaa aagacgtatc
     1321 gttatgatga atagtaaacg ttcctctctt catacggttt ggaaatactt ttttctcctg
     1381 cccgtcctca gcgtgctgat gtgcgcgctg aacaagcccg ccgcacttgc cgcaacggtg
     1441 cgatcctccg cgcccgggca ttccggcact acagcaaacc aggacggacc ggccctgacg
     1501 gccaacccgc tgaagctcct ggcagcgaag gtcgcctcgg catttgggga cacgaccatc
     1561 cggccggcat ccggcaatgc atcttcgagt gaaggttcgg gcaacagtga ttcttcgccg
     1621 gatcaccact attacaatga ggacgactat cagatgctga aagcgtattc cgccggacag
     1681 aatgcctatg atctggccca tctgaatttc aagctggcgg ccgccacaca ggcttccttg
     1741 cagctgcttt tcaaaaaccc ccaaatacat ctcgatgagc aactggtgca ggcggagaag
     1801 atacagctga aaatgctcaa ggataaccag gtgcatttca aattcgatcc ttcgatcaat
     1861 atgaatatgg acatcaaggc taagattttt tcgaagatcg atggttctca tttcgatctg
     1921 tctcatatgg atatgatggc cgatacggga cttcgcgaag gggcctggtt tgccacgtta
     1981 tctgatgaaa aggataagga caagatgcgc atcgaactca gatcgggaga tgataatcat
     2041 aactggaaca gcactttcag cgtggataaa aaagatatca atcctttccc cggtcagggc
     2101 aatgtcgaat tcaagctggt gcgtgaggcc gggaccatct ccttcaaggg tcaattcgac
     2161 gggcaggaag gtttcgggca tttcaagtac acgcccgatg aagggtatct ggctgcgatc
     2221 aaacaaatgg gtgtcgagga tgtggatgac cggcgtcaat tcgcctattt taccctggac
     2281 atcaaaaagg actacgtcaa tatgctcacg catagcgggt atccgcatat cagtcagcac
     2341 gacctgatca gtttcgcggc gatgaagatc gataaggctt tcatcgataa ctggcgcgga
     2401 tcgggttttg aagacgccga caatcccagg aacctgatct cattaaaggc catgaatatc
     2461 gatcgtgctt atgtcgacga gatgaaatca gcgggctatg accatctgag catccatgaa
     2521 ctgatgtctt tgcgcgccat gaaagtcgac agggcctata tccggtcgct tggacgcaac
     2581 agtgacaata cccccatccc cgtccatgag gtgatcagct acaaggcgat gaatatcgac
     2641 agcggttatg tcgaagggct gcgcaaggca ggttatacca acctgacacg gcatgacgtg
     2701 acctcgctgt atgccatgca tgtgacaccg gaattcatca ggggtcttca ggatgccggc
     2761 tataccaata tccccgttca cgagctgacc agtctcaagg cgatgaacgt gacccccgaa
     2821 tttatcaaga gctaccgtga aatgggattc aaggacctca atgtgcgcga gctgaccagc
     2881 ctgaaggcga tgaatatcac acccgagttc atcaagagct tccgcgacat cgggtacacc
     2941 gatctgagcg cccgcgagct gagctcgctg aaggcgatga atgtcacgcc ggaattcgtc
     3001 aatgggttca agaagctggg tttcgaccat atcccggtac atacgctgac ctcgctgaag
     3061 gctacgggcg tggatgcgga ctatgtggcg aagatgaagg cgaagggttt cgtctccgaa
     3121 gacctgaaca agtatatccg gctgaagaac gattttaatt gaccggggcg ggggacggcg
     3181 tatatttgcc gaacggcaag catggccggc aggcaatcgg atcctgcgag gatccggatc
     3241 atggtgacga tctttttctt tgtatcgggg tttgggttca gcacgtgggc atcccgtatc
     3301 ccgacgctcc aacagcaact tcatttgaac gaggcccagc tcggggccgt cctgttcgca
     3361 ttaccgatag gtttgatggg cacgctgccg ctcaccagct atctgctgag ccgttatgac
     3421 agcagacagg tcatgatgat cggggcggtc ctgttcaatg tcctgctttg ctgtatcggg
     3481 ctggccgcac atccctggca actggcgatc ctgctggtct gtttcggatc gtcacgcaat
     3541 ctcatgaat
//
LOCUS       DASYSV010000074         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1040920, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000074 DASYSV010000000
VERSION     DASYSV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040920"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGQ51_02285"
     CDS             <1..552
                     /locus_tag="VGQ51_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV9035413.1"
                     /translation="IRITTDGKPAAGNPFAGRSNAKPEIYSYGHRNPEGLDIDPENGE
                     LWESEFGPHGGDEINLIRPGKDYGWPVITYGLEYSGDKVGEGIQQKAGMEQPVYFWDP
                     VVSPSGICFYKGSGIPEWKDNLFVACLSGEHVDRLIIHNNRVVGEERLLTDKNQRFRD
                     LAYLDGALYAITDDGDIYRISKR"
     gene            complement(589..1035)
                     /locus_tag="VGQ51_02290"
     CDS             complement(589..1035)
                     /locus_tag="VGQ51_02290"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV9035414.1"
                     /translation="MQTESVKTPAGQREETLSVIGHEITVKLSADQTDGTYSVFELIV
                     PPEVGTGLHIDQDWDEYWHVMEGTFAFMLNGERKELPAGGFAFGPKGISHSFRNVGDT
                     VGKLVMVTVPSGLEDFFRHVHQASLKGRPDKEEFVGIMRAHHIEPA"
     gene            1177..>1698
                     /locus_tag="VGQ51_02295"
     CDS             1177..>1698
                     /locus_tag="VGQ51_02295"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV9035415.1"
                     /translation="MVQGEVQKFLRSWPQLTDEQRDTLSRIIPTDRNTVVIFSSCGET
                     VCTWFTSRNFPSVLGHPSGTFVEEGVQFVISRAHPGDLPSVLRFMEASHRPEATWFED
                     PEAGTFQCIYRLKHADGGWRWVTQQLLVLSLTRAGLLDKVLMTFYDCTTQQKKLAERH
                     TAALAVKRTNSRLL"
BASE COUNT          410 a          491 c          420 g          377 t
ORIGIN      
        1 ataaggataa cgacggacgg caaacccgcc gccggtaatc catttgccgg ccgatccaac
       61 gcaaaacccg aaatatattc ctatggccat cgcaaccccg agggcctcga tatcgacccg
      121 gagaacggcg aactatggga atccgaattc ggaccacacg gcggtgacga gatcaatctg
      181 atccgtcccg gcaaggacta tggctggccg gtgatcacct atggtctcga gtactccggc
      241 gataaggtcg gcgaaggcat tcagcaaaag gccggaatgg aacaaccggt ctatttctgg
      301 gaccccgtcg tctcgccaag cggcatctgt ttttacaaag gctccgggat ccccgaatgg
      361 aaggacaatc tattcgttgc ctgtctcagc ggagaacacg tggaccgatt gatcatccat
      421 aataacaggg tggtaggaga ggagcgcctg ctcaccgaca aaaaccagcg gttccgcgac
      481 ctcgcctatc tggacggcgc actctacgcg atcaccgacg acggggatat ctaccggata
      541 tcgaaacgat agccggcagg ttcaatatca acgacggcca actggttcct atgccggctc
      601 gatatggtgc gccctcatga tgccgacaaa ctcttcttta tccggccgtc ccttaagcga
      661 agcctggtga acatgcctga aaaaatcttc cagccctgag ggcacggtca ccatgaccaa
      721 tttgcccacc gtatcaccca cgtttcgaaa gctgtgagat atgcctttgg gtccgaatgc
      781 aaaacccccg gccgggagct cctttcgctc tccgttcagc ataaaggcga atgttccttc
      841 catcacatgc caatattcgt cccaatcctg gtcaatatgc agacccgttc ctacttcggg
      901 cgggacgatg agctcaaata cactatacgt gccgtccgtc tgatccgcac tgagtttaac
      961 cgtaatctcg tgacctatca cggaaagtgt ttcctcccgt tgcccggctg gcgtctttac
     1021 agattctgtt tgcatcgtta taaaagtttt aaagtaatga atgatgcaaa ttatcccgct
     1081 gcacctgcga atgccatccc tgaaaatagt gatttttagg tcgtccggat cccttccgct
     1141 ccccccgtca aagcaaaaaa aacgtaaatt taccacatgg ttcaaggcga ggtacaaaag
     1201 ttccttcgat cgtggcccca actgacggac gagcagcgag atacactcag ccgcatcata
     1261 cccaccgatc gaaatacggt agtcatcttt tcttcctgcg gggagacggt gtgtacatgg
     1321 tttaccagcc ggaactttcc ctccgtcctc ggacacccat ccgggacgtt tgtcgaggag
     1381 ggcgtgcaat ttgtcatctc gcgggcccat ccgggcgatc tgccgtcagt ccttcgcttt
     1441 atggaagcat ctcaccgacc cgaagccacc tggttcgagg atcccgaggc cggcacattt
     1501 cagtgtattt accggttgaa acacgccgat ggaggatgga gatgggtgac ccagcagctg
     1561 ctggtgcttt cgcttacccg cgcaggattg cttgacaaag ttttgatgac cttttatgac
     1621 tgtaccacgc agcaaaaaaa attagcggag cgacataccg ctgcccttgc cgtcaaaaga
     1681 actaacagtc ggcttctg
//
LOCUS       DASYSV010000075         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_914855, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000075 DASYSV010000000
VERSION     DASYSV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914855"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1823
                     /locus_tag="VGQ51_02300"
     CDS             <1..>1823
                     /locus_tag="VGQ51_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV9035416.1"
                     /translation="ADEYFRLLSSMIAGWRREWKQPDLPFYVVQISAFGYASPDNAAK
                     VREAEYEISKRIPHTATAVTLDLGDMHNIHYTHKQEVGLRLAGIALNKDYGFSAIVYR
                     GPEFQSAQLRDDKVEISFAPSTARLAGDAINGFELGYPAPSGDSLLYVPAHARVQGEK
                     VLVWKEGIDHPSEVRYGWALPSEANLFDSEGLPAFPFRAKIDGDNATGKSGAESQNLA
                     LGRPVFANSSDPHYPPSNVVDGRITRESKWQSGNITPPHILEIDLQRYCNIDRVVVHT
                     GIPEKERTPAESQQAAGFWSAKNFKLQYWDDANWTDIPGSEVHENRLTDVAFRLAPAI
                     NTFRLRLVCDDGEPISIMEIEVFGKETSSIPVAGSGTPDLAPKTERSADQRIAIRLTD
                     SVIGKTMRYVGYNQGYYVPGGNTSGWLEYSGVNSLRVWTTLDSYVPAADVQVDKSVTT
                     VEEFDRRKHELRANPEHNTFLHWDHLLPLYEQPEKGGSTNPMVLSYCLSELKRLHIDP
                     VIQAGSTDFDTSWSNKWKQWQRYYALAFYCAKTGDVTMFAMQNEPNHKNSGPMKLDQW
                     IAGMQIVSDALTCAIDDVDRLYGKHLRAKMVGPVTAGNNPE"
BASE COUNT          431 a          553 c          502 g          337 t
ORIGIN      
        1 acgccgatga atactttcgc ctgctcagct caatgatcgc cggctggcgc cgcgaatgga
       61 aacagcccga cctcccgttc tacgtggtgc agatctccgc cttcggctat gcttcgcccg
      121 acaatgccgc aaaggtccgg gaggcggaat acgagatctc caagcgtata ccccataccg
      181 cgacggccgt gaccctggac ctcggcgaca tgcacaatat tcactatacg cacaaacagg
      241 aggtggggct ccggctcgcg ggtatcgcgt tgaataaaga ctacggattt tcagccatcg
      301 tctaccgcgg tcccgagttc caatccgcac agttacgcga cgacaaagtg gaaatcagct
      361 tcgcgccatc cacggcgcga ctcgccgggg atgcgatcaa tggattcgag ttgggctatc
      421 ctgcgccgtc gggcgacagt ctgctgtacg taccggccca cgcgcgggtg cagggcgaaa
      481 aggtgctcgt ctggaaggaa gggatcgacc atccctccga agtacgctat ggatgggcat
      541 tgccatcgga agcaaatctc ttcgacagtg aaggattacc ggcatttcca ttccgggcaa
      601 agatcgatgg cgacaacgcg acaggcaagt ccggagccga atcgcaaaac ctcgccctgg
      661 gccggccggt attcgccaac tcttcggacc cgcattatcc cccatccaat gtggtggacg
      721 gccgcatcac gcgcgaatcg aaatggcaat ccggcaatat aacgcccccg catatcctcg
      781 agatcgatct gcagcgctat tgcaatatcg accgggtggt ggtacacacc ggcattcccg
      841 aaaaagaacg cactcctgcc gaatctcagc aggccgcagg tttctggtcg gccaagaatt
      901 tcaaactgca atactgggat gacgccaact ggacggacat ccccggaagc gaggtgcatg
      961 agaacaggct gaccgacgtc gctttccgtt tggcaccggc catcaatacc ttcaggctcc
     1021 gtcttgtctg cgacgatggg gaacccatca gcataatgga gatagaggtc ttcggcaagg
     1081 agacgagttc gattcccgtt gcgggtagcg gtacgcccga tctggcgcct aaaactgaac
     1141 gctccgccga ccagcggatc gccatccggc tcaccgacag cgtcatcggt aagaccatgc
     1201 gctatgtggg ctacaaccag ggatattatg ttcccggggg gaatacctcc ggctggctgg
     1261 aatattccgg cgtcaacagc ctgcgcgtct ggacgacgct cgattcttac gttccggccg
     1321 cggacgtaca ggtggataag agcgtaacga ccgtagagga gtttgaccgc cgcaaacatg
     1381 agctgcgtgc caacccagaa cataatacat ttctccactg ggaccatctg ctgccgctgt
     1441 acgaacaacc cgaaaaagga ggttccacca atcccatggt cctcagctac tgcctttccg
     1501 agttgaagag gctgcatatc gatcccgtca tacaggccgg cagcacggat ttcgacacta
     1561 gctggagcaa taaatggaaa caatggcaac gctactatgc gcttgcattt tactgcgcaa
     1621 agaccggaga tgtcacgatg ttcgccatgc agaacgagcc caaccacaag aactccggac
     1681 cgatgaagct ggaccagtgg atcgccggca tgcagatcgt ctcggatgcc ctgacctgcg
     1741 ccatcgacga cgtagaccgc ctctatggaa aacacctccg ggcaaaaatg gtgggccccg
     1801 tcaccgccgg caacaatccc gag
//
LOCUS       DASYSV010000076         4898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_16694, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000076 DASYSV010000000
VERSION     DASYSV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16694"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..585
                     /gene="rnhA"
                     /locus_tag="VGQ51_02305"
     CDS             115..585
                     /gene="rnhA"
                     /locus_tag="VGQ51_02305"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEV9035417.1"
                     /translation="MSNQLIMYTDGAARGNPGPGGYGTILMWGDHSKELSCGYRYTTN
                     NRMELMAVIAGLEALKKEGLSVTVYSDSQYVVKAVEQGWLKNWIATDFKGGKKNRDLW
                     LQFYDLSKKYKLRFVWVRGHADNPYNNRCDELATSAADGKDLQIDQVFESEKKK"
     gene            complement(662..1972)
                     /gene="manA"
                     /locus_tag="VGQ51_02310"
     CDS             complement(662..1972)
                     /gene="manA"
                     /locus_tag="VGQ51_02310"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV9035418.1"
                     /translation="MEKDQKVFRLQGKVQHYAWGGTRFLPGLLGIDNSDNRPFAEYWL
                     GAHDNAPAEMGETKAAAAEAAKDGAAGRTDGAAARTDGAAARTGTTLNAYIREHPDDT
                     LGPHTAARFGRLPYLLKILDVNDMLSIQVHPSKRNAELEFAEENRKGIALDAPHRNYR
                     DDNHKPELMLALGEFWLLHGFKPEADLLVILRAVPELRFLIPVFNSHGYQGVYQEVME
                     LPQAEVDAILKPLLARIVPMYQEGELKRNDENFWAARAALTYKNGGKTDRGIFSIYLF
                     NLLNLHPGEAIFQDAGLPHAYLEGQNIEIMANSDNVLRGGLTPKHVDVPELMKHIRFE
                     ATNPRIIFEGDDPGHIAVYRTPAPDFELSKLSLLRGESLTIKAHSAEIFIVLEGSLGV
                     IEPGVPPFGRKKGEAFVSFHQAKFELKAQQDTVVYRASVPAGAL"
     gene            2000..2350
                     /locus_tag="VGQ51_02315"
     CDS             2000..2350
                     /locus_tag="VGQ51_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEV9035419.1"
                     /translation="MPHHIRFGRTGEDLAARWLSEKGFSVLQRNWRYGRYEVDIIAGR
                     QGILHFIEVKSRRSTRYGYPEQSVSQKKLGNVMQGASGWLHRWPAFRRVQYDVLAITM
                     SKDAPPEYMLFEDV"
     gene            complement(2347..2850)
                     /locus_tag="VGQ51_02320"
     CDS             complement(2347..2850)
                     /locus_tag="VGQ51_02320"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEV9035420.1"
                     /translation="MATVNLRSFRKLVLENRRRMRVFLTRLEKKSPRGLDRLKLDSDK
                     GAWLRTDCLDCANCCKTMSPTYTRKDIRRISRHLGMTESAFREKWLYKDKSGDWMNVK
                     QPCQFLDTRTNLCSIYAVRPRDCAGFPHHTTKKMNADYMHMFKQNVEYCPATYRLVES
                     LMEKLKV"
     gene            2878..3783
                     /gene="xerD"
                     /locus_tag="VGQ51_02325"
     CDS             2878..3783
                     /gene="xerD"
                     /locus_tag="VGQ51_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV9035421.1"
                     /translation="MWEPYKKGYKAWLQLERSLSDNSVEAYLRDIDKLTDYLRVSGTL
                     KTPETVDLSDLQQFLQWIAGLELNPASQARILSGIRSFFKYCFIEQIVRTDPTTLLES
                     PKLKRALPDVLSFDEIEQIIGCIDLSKPEGGRNKAILETMYGCGLRVSEVVGLRISCL
                     YLDEGYIRVIGKGDKERLVPIGDSAVKYIRIYQQDIRVHVPAKPGNEDILFLNRRGSK
                     LTRVMIFLLIKELVRQAGISKKISPHTFRHSFATHLVEGGADLRAVQEMLGHESITTT
                     EIYTHLDREYLRETLHRYHPAFSKK"
     gene            3801..4412
                     /locus_tag="VGQ51_02330"
     CDS             3801..4412
                     /locus_tag="VGQ51_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9035422.1"
                     /translation="MGFLRSIPPHNGNTDQADDQTLIAAYRQSADLKVVAALYQRYLD
                     LLYGVCLKYMGEPETAKDSVMDIFEELSQKLLRHEVTYFKGWLYTLAKNHCLMKLRSA
                     GRTRTQSFDPEVMQTEEDPHLMDRMEKEEQFDRLSLCIETLPPDQKAVITLFYLQDKC
                     YKEIETITGMEWNKVRSHVQNGRRNLKLCMQRQEAAARITTNE"
     gene            4405..>4898
                     /locus_tag="VGQ51_02335"
     CDS             4405..>4898
                     /locus_tag="VGQ51_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035423.1"
                     /translation="MNNQDKHITPWSDSDVQKYLKGELSAREMHQLEKAALEDPFLAD
                     ALEGFIQHPAATQDLDELQARLNARVSKKNKSIAWMKMAAAILLLVGLGYTAWYILLD
                     NRKSDLAAFAPRQKHQLSPTAVPPPANSATADSVAPSLASAATADTIAPQATAMAARP
                     SPAKA"
BASE COUNT         1162 a         1526 c         1206 g         1004 t
ORIGIN      
        1 gtgggtagac gcgatcatcg atcccgcaga tacccgccgc gtcatcgccg aaggattgaa
       61 ggcggccgcg cacaacccct ggcttcccga ttttaaaaca ggtgtcatac aggtatgagc
      121 aaccaactga tcatgtatac cgacggcgcc gcccgcggaa accccggccc aggcggttat
      181 gggaccatcc ttatgtgggg cgaccatagc aaggaactct cctgcggcta tcgctatacc
      241 accaacaatc gtatggagct gatggcggtc atcgccggtc tcgaagcgct gaaaaaagaa
      301 ggattgtccg ttacggtcta ttccgacagc cagtacgtcg taaaagccgt ggagcaaggc
      361 tggctcaaaa actggatcgc caccgatttc aaagggggaa aaaagaaccg ggacctctgg
      421 ttacagttct atgatctgtc gaaaaaatat aagcttcgtt tcgtttgggt gaggggccat
      481 gccgacaatc cctataacaa ccgatgcgac gagctcgcca cgtccgccgc cgacggcaag
      541 gatctgcaga tcgaccaggt cttcgaatcg gaaaagaaaa agtaaccacc ggcacccgcg
      601 gcccgacgcg caccctacaa cgccccctcc attcacagcc cagcgcgtca aaagcgccgt
      661 cctacaacgc ccccgccggc accgacgcac gatacactac cgtatcctgc tgcgccttga
      721 gctcgaactt cgcctgatga aaagacacga aggcctcccc ttttttcctg ccgaaaggcg
      781 gtacccccgg ctcgattacc cccagcgatc cctccagcac gatgaagatc tctgcggaat
      841 gcgcctttat cgtcagcgat tcccctcgca acagactcag cttgctcagc tcgaaatccg
      901 gcgccggcgt acgatacacc gcaatatgtc ccggatcgtc gccttcgaaa atgatccgtg
      961 gattagtggc ttcaaaccgg atatgtttca tcaactcggg cacatcgaca tgttttgggg
     1021 tcaatccgcc ccgcaatacg ttgtccgagt tcgccatgat ctcaatattc tgcccctcga
     1081 gataagcatg cggcaatccg gcatcctgga agatcgcttc gccgggatga agattcagta
     1141 agttgaacag gtaaattgaa aagatgccgc gatccgtctt cccaccattc ttataggtca
     1201 atgcggcccg ggccgcccag aaattttcat cgttcctttt cagctcgcct tcctggtaca
     1261 tcggtacgat ccgggccagc aaaggtttca ggatagcatc cacctcggcc tgcggcaatt
     1321 ccatcacctc ctggtagact ccctggtagc cgtggctatt gaacaccggt atcagaaaac
     1381 gcaattccgg caccgcccgc aggatcacca gcagatcggc ctccggcttg aacccatgca
     1441 acagccagaa ttcgcccaat gccagcatca gctctggttt gtgattgtca tcgcgataat
     1501 tccggtgagg cgcgtcgagc gcaatcccct tcctgttctc ctcggcaaac tccaactccg
     1561 cattccgctt gctgggatgt acctggatgg aaagcatgtc attgacgtcg agtatcttca
     1621 acaggtaagg cagcctcccg aaacgggccg cagtatgcgg gccgagcgta tcgtccggat
     1681 gttccctgat ataggcattc agcgttgtac ccgtcctcgc ggcagcccca tccgtcctcg
     1741 cggccgcccc atccgtccgc ccggcagccc catccttcgc ggcttccgca gccgcggctt
     1801 tcgtctcccc catttccgcc ggcgcattgt cgtgcgcgcc cagccagtat tccgcaaacg
     1861 gccggttatc ggaattatcg atacccaaca atcccggcaa aaaacgagtt cccccccagg
     1921 cataatgctg cactttcccc tgtagtctga aaaccttctg atctttttcc atgttatacc
     1981 aaagataaat aacaacccga tgccgcatca tatccgtttc ggacgcaccg gggaagacct
     2041 cgccgcccga tggctctccg aaaaaggctt ctctgtcctg cagcgcaact ggcgctacgg
     2101 ccgctatgaa gtagacatca tcgccggcag acagggcatc ctccatttca tcgaggtcaa
     2161 atcccgtcgt tccacccggt atggatatcc cgagcaaagc gtaagccaaa aaaaactggg
     2221 gaacgtaatg cagggcgcct cgggctggct tcaccgttgg cccgcctttc gtcgggtcca
     2281 gtacgatgta ctggccatca cgatgagcaa agacgccccg cccgaatata tgttatttga
     2341 agacgtctaa accttcagct tctccatcag cgactccacc agacggtagg tagcgggaca
     2401 atactcgacg ttctgtttga acatgtgcat gtagtccgca ttcattttct tggtggtgtg
     2461 atgcggaaaa ccggcgcagt cgcgtggtct cacggcatag atgctgcaca ggttggtcct
     2521 ggtgtcgagg aactggcagg gttgtttcac attcatccag tcgccgctct tgtctttata
     2581 cagccacttc tcgcgaaacg cggactccgt catgccgaga tgcctcgata tccggcgtat
     2641 gtccttgcgc gtgtaggtgg gcgacatggt cttgcaacag ttggcgcaat ccagacaatc
     2701 ggtacgcagc caggcccctt tatccgaatc cagcttcagc cggtcgagac cacgcgggga
     2761 ctttttttcg agcctggtga ggaacacccg catacgacgc ctgttttcaa gaacgagttt
     2821 tctgaacgaa cgcaaattga ctgttgccat aggtgcaaat atagttatct tcggcacatg
     2881 tgggagccct ataaaaaagg ttacaaggcc tggctgcaac tcgagagatc gctctcggat
     2941 aactcggtcg aggcctatct ccgcgatatc gataagctca ccgactatct ccgggtctcc
     3001 ggcaccctaa aaacccccga aacagtcgat ctcagcgacc tgcagcagtt tctgcaatgg
     3061 atcgcaggtc tggagctgaa cccggcttct caggcccgta tcctgtccgg catccgctcc
     3121 tttttcaaat attgcttcat cgaacagatc gtccgcaccg atcccacgac cctgctcgaa
     3181 tcacccaagc tcaaaagagc actgccggac gtacttagct ttgacgaaat agagcagatc
     3241 ataggatgta tcgatctcag caagccggaa ggcggccgca acaaggccat cttagagacc
     3301 atgtacggct gcggtctccg ggtcagcgag gtggtaggtc tccgcatctc ctgtctctat
     3361 ctcgatgaag gctatatccg ggtcatcggc aaaggcgata aagaaaggct ggtacctatc
     3421 ggcgattcgg cggtcaaata tatccgcatc tatcagcagg acatccgggt gcacgtcccc
     3481 gcgaaacccg gcaacgagga tatccttttc ctcaaccgcc ggggcagcaa gctcacccgc
     3541 gtgatgatat tcctgctgat caaagagctt gtcaggcagg ctgggatctc caaaaagata
     3601 tccccgcata ccttccggca ttccttcgcc acccacctcg tcgaaggagg cgcagacctt
     3661 cgcgccgtcc aggagatgct cggtcatgaa agcattacca ccaccgagat atatacccat
     3721 ctggaccgcg aatatcttcg cgaaacgctc caccgctatc accccgcctt ttccaaaaaa
     3781 taattagtat tttgtaagcc ttgggcttcc tccgctccat accacctcat aatggcaata
     3841 ccgaccaggc agacgatcaa acgctgatcg ccgcttaccg gcaatcggcc gacctgaagg
     3901 tggtggctgc gctctaccaa cgctatctcg acctcctcta tggcgtttgt cttaaatata
     3961 tgggtgagcc agagacggca aaagacagcg tgatggatat cttcgaggag ctgtcccaaa
     4021 aattactccg ccacgaggtc acctatttca aaggctggct ctataccctt gctaaaaacc
     4081 actgcctgat gaagttgcgt tctgccggcc gcacccgcac gcagtccttc gaccccgagg
     4141 ttatgcaaac ggaggaagat ccgcatctga tggacagaat ggagaaagaa gaacaattcg
     4201 accgcctgtc actgtgcata gagaccctgc cgcccgacca gaaggcggtg atcacccttt
     4261 tctacctgca ggacaaatgc tacaaggaga tcgaaacgat caccggcatg gaatggaaca
     4321 aggtgcgaag ccacgtacaa aacggccgca ggaacctgaa gctctgtatg cagcgccagg
     4381 aggctgccgc ccggatcaca accaatgaat aaccaggata aacatatcac cccctggtcc
     4441 gattcggacg ttcagaaata cctgaaagga gaactgtctg cccgggaaat gcatcaattg
     4501 gaaaaagccg cactcgaaga cccattctta gccgacgcgc tcgaaggttt catccagcat
     4561 ccggccgcga cgcaggatct cgacgagctg caagccagac tcaacgcccg tgtcagcaaa
     4621 aaaaataagt ccatcgcctg gatgaaaatg gctgcagcca tcctgttgct ggtcggactc
     4681 ggctatactg cctggtatat cctcctcgat aaccgaaaaa gcgatcttgc ggcattcgca
     4741 ccccggcaaa agcaccagct atccccgacg gccgtaccgc cgccggccaa ttcggccacc
     4801 gccgactccg tcgcaccctc gctagctagc gccgccaccg cagacaccat cgccccgcaa
     4861 gcaaccgcca tggccgcacg cccgtcgccc gcaaaagc
//
LOCUS       DASYSV010000077         4440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1425522, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000077 DASYSV010000000
VERSION     DASYSV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425522"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGQ51_02340"
     CDS             <1..289
                     /locus_tag="VGQ51_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEV9035424.1"
                     /translation="SLGCVLYEEKIPVAEDTRQAAFKFELDPTACALSGGEDYELLFT
                     IAQQDYDKLVLNEQISVIGYMTEPEKGSKIITKGGNTFDITAQGWNAFTSK"
     gene            299..1864
                     /locus_tag="VGQ51_02345"
     CDS             299..1864
                     /locus_tag="VGQ51_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV9035425.1"
                     /translation="MKLFCRSYWFLLSFFLTACAGTKDFRADRKYAPDELRKDYTVFR
                     NVLETWHPSLYWYTTKDSMTRYFDEGWAAIRDSMTEEQFRVVLSHVIAQVDCGHTSVR
                     ASKAYAKYLDTARLTQFPLILKCWPDTMVVAANLNRRDSVLRRGTVILSIDGRTPRQL
                     TDTLFPYIVTDGYNLTGKYQYLSTGLHFSSWIKEILGSADSFDIGYLDTSGIAREIRI
                     PLYNPRADTLRGSLGRVLLPGTGQRGQGPPRRLRPPSRKRRKERERLLNRDFRVDSAT
                     ETAFLTLNTFESGYGLRGFFRHSFGEMKDRRIKNLVIDVRSNGGGEASLSTLLTRYLI
                     DKKFKLADSLYTVRRHSKYDRYINNGFLYRLLTIVTTSKRSDGFYHFGYFERHYYTPL
                     KKLHFDGRVYIMTGGNSFSATCLFAGALKGQSNVTLVGEETGGGSYGNTAWMIPDVTL
                     PVTKVRFRLPRFRLVVDKQRVKDGRGILPDVEALPTAQAIGKGQDYKTAAVKELISTN
                     ATSPHTAESKGHP"
     gene            complement(1867..2280)
                     /locus_tag="VGQ51_02350"
     CDS             complement(1867..2280)
                     /locus_tag="VGQ51_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9035426.1"
                     /translation="MIFSKDYPLNILIAENNPDARASTTTIITELGYQPRVAASTEEL
                     LHLTCTESYDVILMDIHMPAVEDMLATGLSGRKDGRPLIIGITGNARPGFKQICLQAG
                     MDHHIRRPVDPQELKLQLKACSVLSGNRRIRTAGE"
     gene            complement(2319..3362)
                     /locus_tag="VGQ51_02355"
     CDS             complement(2319..3362)
                     /locus_tag="VGQ51_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV9035427.1"
                     /translation="MKWSYVISFAAGILFFTLLTWSFWWLVGGAVILVGAWVYQMVAE
                     RRYMHAVKTATLENQLRELQAKLDRSVYREQQLSRIAEQAMTAKKQWLATLNQEIRTP
                     VNGMAGMASLLADTSLNDQQLEYINGIQHCGEQLVAVVNHILVDDLLNTSLIGTDDGQ
                     PQEDHLDLRGAIKDVLDLFAGRVGDKGPVLSAHIDDQIPQQLAGEGGRIKQILINLVD
                     NAVKNTSQGEISIDARLLKIKEDAPLELGFEVRDTGNGIPASRIGKIFEGLSPVAPGD
                     ENPGLGLVVCRRLVEMMDGSIEVRSEPGKGCTFSFHLCLNAAGVQPSRQPISAATSAT
                     VLTDEVRRLALKV"
     gene            complement(3506..>4440)
                     /gene="nagA"
                     /locus_tag="VGQ51_02360"
     CDS             complement(3506..>4440)
                     /gene="nagA"
                     /locus_tag="VGQ51_02360"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV9035428.1"
                     /translation="IYGGNGRLFSAELTTEALEAVYEYCLQGGCTQFLITLATNSIDK
                     FIRAMEVAREYRISGGKGLLGVHLEGPYINPAKKGAHLEKFIRRPTPEEIELLITKGK
                     DVLRMMTLAPEQCDPECIELLLRNGIVVSAGHSNATYEEATDGFYKGIPAATHLFNAM
                     SPLQGRQPGMVGAIYDHNDVLTSVVCDGIHVDFASVRISKKILGERLFFITDAVAEVQ
                     YGEYVHVFRGDRYTLPDGTLSGSALTMLQAVRNGVEKTGIPLPEALRMASLYPAQLIG
                     LQDRLGSICPGASADLILLDEQLNLLQVFAEGEE"
BASE COUNT         1019 a         1223 c         1178 g         1020 t
ORIGIN      
        1 cagtctgggt tgtgtgctgt acgaggaaaa gatacccgtc gcggaagata cgcggcaggc
       61 agctttcaag ttcgagctcg atccgacggc ctgcgcgctc agcggcggag aggattacga
      121 actccttttc accatcgccc agcaggatta cgataagctg gtcctcaatg aacagatcag
      181 tgtcatcgga tatatgactg agcctgaaaa agggagcaag atcatcacca aaggaggcaa
      241 cacgttcgac attaccgcac agggatggaa cgcttttact tccaaatgaa cccaccacat
      301 gaagctattt tgccgatcct attggttcct cctctctttt ttccttaccg catgcgctgg
      361 cacgaaagat ttccgcgccg accggaaata tgctcccgat gagctccgaa aggattatac
      421 cgttttccgg aatgtgctcg agacctggca ccccagtctt tactggtata cgacaaagga
      481 tagcatgacc cgctatttcg acgaaggatg ggctgccatc cgggattcga tgacagaaga
      541 gcaattcagg gttgtcctga gtcatgtcat cgcacaggtg gattgcgggc atacttcggt
      601 aagggcttct aaggcctatg ctaaatatct ggatacggca aggctgacgc aatttcccct
      661 tatcctgaaa tgctggcccg atacgatggt ggtggccgcc aatctcaacc gccgggattc
      721 cgttttgcga agagggacgg tgatcctgtc catcgacggc aggacaccgc ggcaattgac
      781 ggacacgctg ttcccctata tagtgacgga cggttacaac ctgacgggaa aataccaata
      841 cctgagcaca ggtttgcatt tcagctcctg gatcaaggag atactcggat ccgccgattc
      901 cttcgacatc ggctatcttg atacatcggg aatagctcgt gagatccgca tcccgctgta
      961 caatccgcgg gcggataccc tgaggggaag tctcggacga gtgctccttc cgggcacggg
     1021 tcagcgggga cagggtcccc cacggcgttt gagaccgccg agccggaaaa gacgcaaaga
     1081 acgcgaacgg ctgctcaacc gggattttag agtggacagc gccacggaaa cggctttcct
     1141 cacgctcaat accttcgaaa gcggttatgg gttacgggga tttttccgtc actccttcgg
     1201 tgagatgaag gatcgacgga taaaaaacct tgtgatcgat gtccgctcca acggaggggg
     1261 agaagcctcg ttatcgaccc tgctcacgcg gtatctcatc gacaaaaaat tcaagctggc
     1321 cgattcgctc tataccgtcc gccgccatag caaatacgac cggtacatca acaacggctt
     1381 tctgtatcgc ctcctgacta tagtgacgac ctccaaacgc tccgacggat tttatcattt
     1441 cgggtatttc gaacgacatt attacacgcc gctgaaaaaa ctgcacttcg acgggcgggt
     1501 gtacatcatg accggaggga attcattttc ggcgacctgt ctttttgcgg gggcgttgaa
     1561 gggtcagtcc aatgttacgc tggtgggtga agagacgggt ggaggatctt atggaaatac
     1621 tgcctggatg atccccgacg tcacgttgcc tgtaacgaag gttcgcttcc gtctgccgcg
     1681 gttcagattg gtggtggaca aacagcgggt gaaggatggc cggggcatcc tacccgacgt
     1741 cgaggcgctg cctaccgcgc aagccatcgg caagggacag gactacaaga ccgccgccgt
     1801 taaagagctg atcagcacaa atgccacgag cccgcacacc gcggaatcga aaggccatcc
     1861 atagggtcac tcgccggcgg tgcgaatccg ccgattcccc gacaacacgg aacaggcttt
     1921 gagctggagc ttcagctcct gcggatcgac gggtctccgg atatggtgat ccatccccgc
     1981 ctgcagacat atctgtttga aacctggccg ggcgttgccc gtaataccaa tgatgagcgg
     2041 ccgtccgtct ttcctgccgg acaggcctgt ggcgagcatg tcttctacag caggcatatg
     2101 gatatccatc aggatgacgt cataagattc ggtgcacgtc agatgtagca gctcttcggt
     2161 gctcgccgcc acccggggct ggtatcccaa ttcggttata atagtggtag tagacgcccg
     2221 ggcgtccgga ttattttcgg cgataagtat attaagagga taatctttcg aaaagatcat
     2281 agggttttgc atggatatca aacattcaga tgccggggtt agaccttaag agccagacgc
     2341 cggacctcat cggtgaggac ggtcgcggag gttgccgcgg agatcggttg ccgcgaaggt
     2401 tgaaccccgg cggcgtttaa acacagatgg aagctaaagg tgcatccttt acccggttcg
     2461 ctccggacct cgatggaacc gtccatcatt tccaccagcc tgcgacatac caccaatccg
     2521 agaccgggat tttcatcacc cggcgccacg ggggaaagcc cttcgaatat ctttcctatc
     2581 ctggatgccg gtatcccatt gccggtatcc cgcacctcga atcccagctc cagaggggcg
     2641 tcttctttta tctttaacag acgggcatct atgctgatct cgccctggct ggtattcttc
     2701 acggcattat ccaccaggtt gatgaggatc tgtttgatcc ttcccccttc tccggccagc
     2761 tgttgcggta tctggtcgtc gatatgtgcg gaaagcacag ggcctttatc ccccaccctt
     2821 ccggcaaaca gatcgagaac gtcttttatg gcgccgcgga gatcgagatg gtcttcctgc
     2881 ggctgaccgt catccgttcc gatgagggaa gtattcagca gatcgtcgac aaggatatga
     2941 ttcaccactg ccactaattg ttcgccgcag tgctggatcc cgttgatata ttcaagctgt
     3001 tggtcgttca gtgaggtatc ggccaataac gaagccatgc cagccatacc gttcacggga
     3061 gtgcggatct cctggttcag cgtggccagc cattgctttt tggcggtcat ggcctgctcc
     3121 gctatcctgg atagctgctg ttcccggtaa acggatctgt ccagtttggc ctggagttcc
     3181 cgaagctggt tttcaagggt cgcggtcttc accgcgtgca tataacgccg ctcagcaacc
     3241 atctgataga cccaggcgcc aaccaaaatg acggcgccgc caactaacca ccagaaagac
     3301 catgtgagta aggtgaaaaa aaggattcct gcggcaaaag agataacata agaccatttc
     3361 ataacaatac gtgacatttg ggtgagggga tatccttaac aatataacag agggtagacc
     3421 tttttatgac cgaccgccgg tcgaattttt tcttaatagg agaaagcgca actaaaatta
     3481 cgtgattcca cttaattcat tagacttatt cttctccttc cgcaaaaacc tgcaacaaat
     3541 tcaattgctc gtccagcagg atcagatcgg cgctcgcccc cgggcaaatg ctacccaacc
     3601 ggtcctgcaa tcctatcagc tgagccgggt acagtgatgc catcctcaga gcttcgggca
     3661 gtgggatccc cgtcttttcc acgccattgc ggacggcctg caacatcgtc agggccgatc
     3721 cggaaagggt gccgtcgggc aacgtatagc ggtctccccg gaatacatgc acatattcgc
     3781 catattgtac ttccgctacg gcatccgtga taaagaacag cctttcccct aggatctttt
     3841 tgctgatgcg gacggaagcg aagtcgacat gaatgccgtc gcatacgaca ctggtgagga
     3901 cgtcattgtg gtcataaata gccccgacca tccccggctg acgcccctgt aatggcgaca
     3961 tggcattgaa aagatgtgtt gctgcgggga tacccttgta aaagccgtct gtcgcctctt
     4021 cgtatgtagc attgctatgc cccgccgaca ccacgatccc gttccggagc aggagctcta
     4081 tacattcggg gtcgcattgc tcgggtgcaa gggtcatcat cctcaataca tccttccctt
     4141 tggtgatcag gagctctatt tcttccggtg tcggccgccg gatgaatttc tccaggtgcg
     4201 cccctttttt cgcggggttg atatagggac cctccaggtg cacaccgagc aggccctttc
     4261 ctccgctgat gcgatattcc cgggcaactt ccatagcccg gatgaatttg tcgatggaat
     4321 tcgtggccag cgtgatcagg aattgggtac agccgccttg cagacaatat tcatacacgg
     4381 cttccagcgc ttctgtggtc agctcagcag aaaaaagtct gccgttacca ccatagatct
//
LOCUS       DASYSV010000078        18827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_400472, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000078 DASYSV010000000
VERSION     DASYSV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 18827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18827
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400472"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VGQ51_02365"
     CDS             <1..818
                     /locus_tag="VGQ51_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEV9035429.1"
                     /translation="RTLHISPRQQDLEVDGRRVRIGLFPMGIDYRLICEVSRSVRCQE
                     LKTAILNDFSGRKLILSVDRLDYTKGILNRLMAFDRLLRDDPGLKGKLLLMLVVAPSR
                     REIPSYQHIKRNIEEWVGRINGAHGTNSWVPVVYQYRQFDLTELCALYAASDVALVTP
                     LKDGMNLIAKEYIASHTDRLGVLILSEMAGAIHELTDALSVNPYSVEEMAATIKEALM
                     MDVDEQRFRNERMHRRLEAYDVVKWAHDILETTLSLNRAGQLGGTPAGETLLA"
     gene            815..1777
                     /locus_tag="VGQ51_02370"
     CDS             815..1777
                     /locus_tag="VGQ51_02370"
                     /inference="COORDINATES: protein motif:HMM:NF042586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6734 family protein"
                     /protein_id="HEV9035430.1"
                     /translation="MKIIQSFWTLPMLKREAAAPENRFSGGWCEMKYHLMSWAYSCLQ
                     LRCFYDEVELITDQAGKELLIDRLQLPYTHVKVVLDNLNGYDPNWWAIGKVYAFGLQD
                     QPFLHVDGDVFIWEPFPERLVKAALVSQHLEYAYPFYKEVLGFLLNRGAYIPKDILSG
                     AEGQTALHAFNGGIIGGNDLAFFKEYVLEAWKFIRQNQAISDAPNAGNLNAFYEQHLF
                     YCMAARRKIPVECYTYETDEDRLWDLFKSVQRFGDAPAGGGYIHLYGEAKKMVRHCEI
                     LAERLRQEHQRYYYRVLNALTSDFNENQYDDEITNAAGDMAPGL"
     gene            1728..2096
                     /locus_tag="VGQ51_02375"
     CDS             1728..2096
                     /locus_tag="VGQ51_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035431.1"
                     /translation="MTTRSLTRLEIWHQDYNLGYKHNLDELPSGKAVFGVFAIVNDLP
                     VNCRYAAETENLRDSVQRLFEHPEGKGLRKFMQGPWIQMLVYESMPDSEEGERRTVLE
                     EWIRNYRPAIDEDGEYPGYY"
     gene            2117..2386
                     /locus_tag="VGQ51_02380"
     CDS             2117..2386
                     /locus_tag="VGQ51_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035432.1"
                     /translation="MAILGNVEEIQVVKERLETLKGQGLIKEWEVPYEHLLTRLSAAV
                     FFLTPSEPSRTEDIRTALSCYPNLHYGVNENKQLSRMDWKFEYHP"
     gene            2383..3105
                     /locus_tag="VGQ51_02385"
     CDS             2383..3105
                     /locus_tag="VGQ51_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV9035433.1"
                     /translation="MKKIKLFCLPYAGGSAMVYNRWREYTDPAIEIVPVELAGRGARM
                     NEPFYADIRHAAHDVKHFIKKEAAGGSYALFGHSMGGLIAYLVARELEDQEFPPAAHL
                     FISGTGAPHIARNDKKYHVMEEGEFRKEVLSLGGTPTELFEYPELLELFLPLLRNDFR
                     LVEQYVPAEVIRPLEKEITILFGKDDDLKPDECDGWKMHTTQVCRMYHFAGGHFFIQD
                     EMEKITHLINTTLCHSPILQQY"
     gene            3075..9437
                     /locus_tag="VGQ51_02390"
     CDS             3075..9437
                     /locus_tag="VGQ51_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEV9035434.1"
                     /translation="MPFTHLAAVLKERSDCFDRGITFIDGEGKEDFLSYGDLYRNALK
                     VLSWLQSKGLRQGSELVFQVEDNRTFVIAFWACLTGGLIPVPLTPGKTADHRQKLFGV
                     WSLLRQPALLISRNMLSQIGEFATLKGLEALYADMMAGMIAEEELFSGTEDGQPVDVR
                     EEDIAFIQFSSGSTGNPKGVILTHRNLLTNIRSIGKAASYAMDDVVLSWMPLTHDMGL
                     IGFHLNPLFVGMNQFLMPAGLFVRRPALWLDKATEHKATIMSSPNFGYEYIVKHCLKE
                     GAWFDWDLSAVRLLYNGAEPVSEKICRHFLDTFHVYGLRRNSMCPVYGLAEASVAVTI
                     SEPAEEVKAVVVSRHHLKLGDNVTEPESEADEVSFVNVGKPIDDCQLRIMDDDDREVD
                     PGVIGHIQIRGGNVTRGYYNNDAETRRVLTEDGWLRTGDLGFLRQASLYVTGRWKDIF
                     FINGQNYYPHDIESIACEVDGVELNKIVIVGRRDDANSREEAIAFVLHRGETADFLHV
                     ALAIRNTVNREAGIVLDKILPVKDIPRTTSGKLQRYKLLQQLMDGELEEADRLITPLL
                     SSSGIIGGAVPRGGEEAAVLELWKEISGNPDAGPDDRFFETGGNSLKAVELSMAVLKV
                     FQVELGPELIYQKQTAKLLAEAIRAGVKKPYTAIPVGPQLDHYPLSGAQERLYYIWKA
                     APSSIAYNLPVAFRLRGRIDTDKLEACIRKMILRHDAWRSVFLEEAGPQLSIREDMPF
                     DLSCRNCHPDELDATLRSLVRPFHLLEGPLFRITLVKTGDSEQVLLADFHHSISDGIS
                     LYRCAEELMSLYCDRQPPELPVGYKDYILWEKGEGHIRPYAAQQAFWLEELKGPLPVL
                     EMPIHSLRPPVFQTEGGKMEFHLPGKLVSGLHGVALRQRCTMHVLLLTLYKILLWKYT
                     GQEDLIAGVPVAGRNHPDLRHLQGMFVNNLVIRSHVRGEDNFLSFLELQRARISAILA
                     NQEYPFDRLLSQLSLKRDSSRNPVFDTMFNYQNMGDPAREAGGIIMSRYSFDPGFAKF
                     DISMDVFEQDNTLVYGIEYCTHLFERKFILDLARHFQHMTERVAEDPDRSVGELAALP
                     DEETERYIYRWNASGADYPRAMTVHQLFEEQAGKTPDRIAIEWDGKEISYRELNERAG
                     SVARWLADQHLGKEEIVALHMPRCSELIVAMLGIWKAGGAFLPIEMELPERRKQVILQ
                     KSGCRLVITENHLPEEIRPHEPPVPTPASLAYVIYTSGTTGEPKGVAIEHASLVNYIH
                     WAAGQYLQGERHAFPLFTSIAFDLTLTSIFVPLVTGNRIVIYNQRQGEVLLERIIADN
                     KVGIIKLTPSHLRLLRDGCRRSEGSRIKRLIVGGERLETQLAAAITQLLGGDVEIYNE
                     YGPTESTVGCMIHRYRSEEKRIGVPIGIPIANTRIYLLDGQHRPVPEGVKGELYIAGR
                     GLARGYLHDEGQEKFMDDPFFKGEKMYRTGDLAIRLPEGYLDYMGRCDRQVKINGYRI
                     ETGEIAGVLSGLPEIGDVEVSVRKGEEGKENLYAYYTLTDNRQEALPETWLRDYLAER
                     IPYYMIPARFILLKKMPLNGNGKIDMDALPLPETQLREQRRPIGTWEELSVKIWEEIL
                     GREGIGVRDSFFELGGDSIKAVQIVGRMADAGVSLQVRDILTYHSIEQTCRHAHAVQG
                     RWYHQGRAEGEKERSPIETWFFDLALKDPSFYNQSVLFRLGRRIPKALLEEAFRLLIN
                     HHDTLRMNVDVKTGRLFYNNRHAETEFSIGEGGSTADLLPLAGGFDLGSSLLLRASLL
                     ADTDAGNYLSITAHHLIMDGVSWRIFLEDLYSVSTALEKGEEPKLPRKTASLKDWQAE
                     WRRHADTLLPEEEAYWTRVEEIRFALPVDLQTNDWRAKHLATQSGRLSPEETRLLLQA
                     HAVYRADISTILLTALAAVLKEWTGSDHCVLEMEHHGREMEFADVSRTLGWFTSLYPL
                     HLRCPEGTEDRRVSAIWQQVKEVPRQGIGYGAGPRKRRKGSEDCAPRTEIRFNYLGRF
                     DTEFDNDLFQYIPSSGGIGTDPENSMTAKLEMIAMIFGGQLIVDVHYNRLAHEDGTIR
                     TFMEGFLRHLRRLVGPTDKNDQAGEFLPSDFGAVELNEKDWKTLFG"
     gene            9468..10322
                     /locus_tag="VGQ51_02395"
     CDS             9468..10322
                     /locus_tag="VGQ51_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEV9035435.1"
                     /translation="MNMVAVVGAGVMGADVALTLSCYGYPVVLKDIRPDALQQARERI
                     CRDLRMVRMMKPVFKNLAAEEVMGRIRFALDYEGFERADIVIENVSETWTVKAGVYQE
                     LKGVCHGNVLVCVNTSCISITRIAALLDHPQNVIGMHFMNPVPLKEIVEVVKGYHTSP
                     DTIDRAKAFLRCLEKTPVLVNDLPGFVANRLSHLFMNEAAFLIQDQVASAREVDLIFK
                     KGYGHSMGPLETADLIGLDTVVSSLEVLYTSYQDPKFRCCPLLRKMVDAGLLGKKSGE
                     GFFKYQDE"
     gene            10319..11383
                     /locus_tag="VGQ51_02400"
     CDS             10319..11383
                     /locus_tag="VGQ51_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEV9035436.1"
                     /translation="MTHLKEVKCVVWDLDNTLWCGTLLEPGDVLLRPGIREVIAELDG
                     RGILHSIASRNNYQDAMGKLREWDLHDFFLYPQINWGSKSESIRLIQEKLNIGMDTIV
                     FIDDQPFERDEVSESLPEVETFDASTYLDLPALTRMNPRFVTRDSKQRRIMYQEDMQR
                     VKDEEEYIGPRESFMASLRMRFIISEAEEEDLERAEELTVRTNQLNATGRTYSYEELY
                     ALMKSDHHKLYVCELTDRYGSYGKIGLALVEMQADHWCLNMLLMSCRVMSRSVGSILL
                     SYIMQRARQDGKKLLAHFKQTDRNRMMYVTYRFSNFSELTNDGHGNIVFANDLSQVQG
                     FPPYVEVMTPGAAGFPHSQA"
     gene            11395..11643
                     /locus_tag="VGQ51_02405"
     CDS             11395..11643
                     /locus_tag="VGQ51_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV9035437.1"
                     /translation="MTIREKIRSFIGANLLVSGEDEEFSDADNLFQLGYVNSLFAMKL
                     LNYVEREFDITVDNEAMDIRNFSSVLNIVSLVEKIRQT"
     gene            11640..12803
                     /locus_tag="VGQ51_02410"
     CDS             11640..12803
                     /locus_tag="VGQ51_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV9035438.1"
                     /translation="MTDSLQDRILAEAKDFADREIRPFAAVFDKNQGISRDLIGKMAE
                     RGYLAPCLPRLYGGLELDALHYGLFTEIIGKACPSTRVLLTVHCSLIGETILRWGTME
                     QKNRILPELAGGRRLAAFALTEPGTGSDAKNVQTLYRQQGDHYHITGKKKFISLGAIA
                     DLFLVLARGAAGEVTAFLIDRNSPGISIEPMTGLLASRAAYVAAIGFSDVCVPAENVL
                     GKEGGGFTFIVNTALDYGRYSIAWGGLAIAQESLDVMVAYARNRKQSGRPIHTFQLIE
                     GIIGDGVARVEAARSLCMHAARLRDQRHEDAVIHTTIAKYFASRAANRLASDAVQVHG
                     GNGCYDEYPAERLFREAKVLEIIEGTSQVLQAVIAEYGLRKFYAGPSIKSTSL"
     gene            12800..>18827
                     /locus_tag="VGQ51_02415"
     CDS             12800..>18827
                     /locus_tag="VGQ51_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEV9035439.1"
                     /translation="MKAKIEKSNVLEIFELSSIQKGILFHYLRDSGSHIYNVQLVFDI
                     RGSLDKDRLDRAVAAVQRQNPVLRSVFSWEKVSKPLQILLKTYPRPFEYIDISGIGTQ
                     ERQASLQRCLEEDRQERFDLETLALRLKLIKTSPDTFVFSITHHHILYDGWSTGLLLT
                     ELFLAYRHPDREPIKPDYPGIYRRHAGRNLPEDGRRYWRAYLEDYQMHSLLPPIRDRN
                     HTVNSFRVGRPMAALDRFSAQHRVTKAALIYAAFGLLLQKYANSSDVVFGTPVADRDP
                     AIPGIERLIGNFINTLPLRVTATDEATLLDIVLRVHADMADRHEHSHHSYAEIKDVLK
                     LRPDENLFEITVAVENYPLDKTAIEGIEGLSVTLRSSYEFTDSPIAVNVFFGADLGVS
                     IAYDLAFADEAFARQLAEHFLAILDKICDDPFCLVHRLDILSDAERRLLLETFNDTAA
                     EFPETETIVSLFEKQVSRTPDNTAICCDGKIFSYRTVYEEMNRIAAGLTTTYALGAGD
                     VAGVLMMKSERLLITILAILKTGASYLPIDLSNPPDRVAYMVEDSDMKLLITDQPSVS
                     AITPSGLSVLGYETLQAAAGWSGNMTGAPCWPDYLAYTIYTSGSTGMPKGVGLKHRGF
                     VNLIYWYAKALAGTETDNYLLIAPVSFDLAQKNIFTPLLTGASITLSEDIQLDYGRMA
                     ATIASKGVTVINCAPSAWYPLLEEAAHNGYRDLAGVRKVVLGGENIHMKAFEGWFHSG
                     NFHAQLINSYGPTECTDVVAWHQLEDPVHRAYDRVPIGRPVDNMRLYILDRQGALQPL
                     GALGEICIGGTGVSDGYRNNPELTRRKFFPDAISGKGMLYRTGDAGRWLRNGEMEFIG
                     RIDNQVKIRGFRVEPGEIEVQLSRHERVRQCAVIVGEKEAEKFLVAYYVADHPLESAA
                     LRAFLSARLPHYMVPAWYVHLTSLPRTPNGKLDRRALPSPQIRAESEQTVPTRAMEKK
                     LLAIWCDVLGQTRIGVTDNFFDVGGDSLKLITVSSRIRVDMEKQVPVTELLQYPTIRT
                     LADHLQPAVQKNREQSNAEPSAAAKDIAIIGMACRFPGAPDVDAFWGNLRAGRESITR
                     EGDADISGEYRLVRAKGLLEGYECFDAAFFNYSPADAKLMDPQIRIFHETVWEALEHA
                     GYRCPSFPGRIGLYAGATPSPYYELDTSKPQGDQLLEEWASLSYADKDFLCARVSYKL
                     NLRGPSLNINTACSTSLVATDLACQELMAGKCEMAVAGGISVTLHDNEGYIYHKGMVM
                     SPDGRCRAFDAEAAGTVGGNGSGAVVLRRLEDALRDGDTIYAVIKGTATNNDGNRKVG
                     FTAPGVDGQAAVISHAMRKAGVRPEDIGYVEAHGSGTLLGDPIEVAALVEAFGTDKRA
                     YCGLGSVKTNIGHLDAAAGIAGLIKTVLALYHGEIPPSLHFHQPNPNLELGASPFYIN
                     DRLRDWQKDGRPRTAGVSSFGIGGTNAHIILQEAPPRSAGSASRSHQLLLLSARSENA
                     LAANSARLADHLQAMADEEHSGRTAEQQALADLAYTLQTGREPMPYRRMIVCDTVVQA
                     IEQLSIFPVNGKTSPLDESLANRVVFLFPGQGAQYSGMCRGLYEKEPYFREQMDSCLA
                     IVRQLSGKDLKPLIFGGSRRLHDTETAQPALFVVEYALARLLMKWGILPHLMIGHSFG
                     EYVAACLSGVFSLEDALSIVVKRGELMQQAPGGISLGVAVSEEVLTSWLEKYPGVSLA
                     TVNSSELCVVSGQAEQIEAFRKEMVICGYPSEVVSEAHAFHSSRMDGILEAFASHIRQ
                     IHPATPRIPFVSNLTGEVATADAVTGTAYWVDHLRKTVRFADGIRTLMKEEHMVFVEV
                     GPGIELSSFVRSMRERGPGHKVINMVRHAQTEGDDQYHLEKGIGRLWLIGIEPAWGSF
                     YEGETRRRVPLPSYAFDRVCFPVRNKRPVAGTAKTEEDLVRSKDISRWWYAPTWRHVP
                     LLPEPAGSDGSCTLLLDDDLWIGAGVAEVLRGYGERVILVRKAAAYKNDGD"
BASE COUNT         4436 a         4681 c         5670 g         4040 t
ORIGIN      
        1 tccggacact tcacatcagc ccacggcaac aggacctgga ggtggatggc cggagagtca
       61 ggataggcct gtttcccatg ggtatcgatt accggttgat ctgcgaagtt tcgcgatcgg
      121 tccgctgcca ggagttgaaa acagccattt taaatgattt ttccggccgg aaactgatcc
      181 tttccgtgga ccggctggat tataccaagg ggatcctgaa ccggctgatg gcattcgatc
      241 gactgttacg ggacgatccc ggattgaagg gaaaactact tttgatgctg gtggtggctc
      301 cttcccggcg ggagatccct tcgtatcaac atataaagcg caatattgaa gaatgggtgg
      361 gccgcatcaa cggggcgcat ggtaccaata gctgggtgcc ggtggtatat caataccggc
      421 agttcgacct gaccgagcta tgtgcgctgt atgcggcgag tgatgtggcg ctggtgactc
      481 cgctgaagga cggcatgaat ctgatcgcca aggagtacat cgcatcgcat acggacaggc
      541 ttggcgtact gatcctcagt gaaatggccg gggctatcca tgagctgacg gatgccctat
      601 ccgtgaaccc gtatagcgtg gaagaaatgg cggccaccat aaaggaagcg ctgatgatgg
      661 acgtggacga acagcgcttc aggaatgaac ggatgcatcg gaggctggaa gcctatgatg
      721 tcgtcaaatg ggcacatgat atcctggaga cgaccttgtc cctgaaccgg gcgggacagt
      781 tgggtggaac gccggccggg gaaacattgc ttgcatgaag atcatacaaa gtttctggac
      841 gttgccgatg ttgaagcgtg aagctgccgc gccggagaac cgctttagcg gcgggtggtg
      901 cgagatgaaa taccacctga tgagttgggc atacagctgt ctgcaactgc gctgtttcta
      961 cgacgaggtc gagctgatca cggaccaggc gggaaaagag ctgctcatcg acagactaca
     1021 gcttccttat acgcacgtca aggtagtcct ggataacctc aacgggtacg atccgaattg
     1081 gtgggccatc gggaaggtat atgcattcgg gctgcaggac cagcctttcc tgcatgtcga
     1141 tggagatgta ttcatctggg agccttttcc cgaacggctg gtaaaggctg ccctggtgag
     1201 ccagcacctg gaatacgcct atccatttta taaagaggtg ctgggcttcc tgctgaatcg
     1261 gggcgcctac atcccaaagg acatcttatc cggagccgag gggcagaccg cgcttcacgc
     1321 attcaatggc ggtatcatcg gaggcaacga tctggcgttt ttcaaagaat acgtcctcga
     1381 agcatggaaa ttcatccggc agaaccaggc catatcggat gcccctaatg caggcaattt
     1441 aaatgcgttt tatgagcagc acctgttcta ttgcatggca gcccggcgga agataccggt
     1501 ggaatgctac acgtacgaaa ccgacgagga ccggttgtgg gatcttttta agagcgtgca
     1561 gcgatttgga gatgcacccg caggtggcgg ctatatccat ttatacggcg aagccaaaaa
     1621 gatggtccgg cactgtgaga tactggcaga acggctgaga caggaacatc agcgttatta
     1681 ttaccgggta ttgaacgcat tgacgagcga ctttaacgaa aatcaatatg acgacgagat
     1741 cactaacgcg gctggagata tggcaccagg attataacct tggctacaaa cacaacctgg
     1801 acgaattgcc atccggaaaa gccgtattcg gcgtatttgc catcgtaaac gatctgcctg
     1861 tgaattgccg ttatgccgcg gagacggaaa acctgcggga ttcggtccaa cggctgttcg
     1921 agcacccgga aggaaaaggt ttacgaaagt tcatgcaggg gccctggata cagatgctgg
     1981 tatacgagtc gatgccggat tctgaggaag gagagcgacg gacggtcctc gaagaatgga
     2041 taaggaacta tcggcccgcc atagacgagg acggagagta ccccggatac tattgatctt
     2101 acaaaaaaca tttgtcatgg caatacttgg aaacgtggaa gaaatacagg tggtaaaaga
     2161 acggttggag accctaaaag ggcagggcct gatcaaagag tgggaggtgc catacgagca
     2221 tcttcttacc cgattgtcgg ccgccgtttt tttcctgacg ccttcggagc cctcccggac
     2281 cgaagatatc cggacggcat tgagctgcta cccaaacctt cactacggcg tgaatgaaaa
     2341 taagcagctt tcccgaatgg attggaagtt cgaatatcat ccatgaaaaa gataaaactt
     2401 ttttgtcttc cttatgcggg agggtcagcg atggtctata ataggtggag ggaatacacc
     2461 gacccggcca tcgaaatagt tcctgtggaa ctggcgggaa ggggtgcgcg gatgaacgag
     2521 ccgttttacg cagatatcag gcatgccgcc catgatgtga aacatttcat caaaaaggaa
     2581 gctgccggcg gaagctatgc cttgttcggt cacagcatgg gcggcctgat cgcatacctg
     2641 gtcgcccggg aattggaaga ccaggaattc ccacctgccg cgcacctatt catttccggg
     2701 accggcgctc cgcacatcgc gcgcaacgat aaaaaatatc atgtaatgga agagggagag
     2761 ttccgcaagg aggtacttag tcttggcgga acgcctacag agctttttga atatccggaa
     2821 ctcctggagc tgttcctccc attgctacgc aacgatttcc gactcgtgga acaatatgtg
     2881 ccggcggagg ttattcgtcc gttggagaag gagatcacga tccttttcgg caaggacgac
     2941 gacctgaaac ccgacgagtg cgacggctgg aagatgcata ccacccaggt ttgccgcatg
     3001 taccacttcg ccggcggaca tttctttata caggacgaaa tggaaaagat aacccatttg
     3061 atcaatacta ccttatgcca ttcacccatc ttgcagcagt actgaaagaa aggagcgact
     3121 gtttcgaccg gggtattacc ttcatcgacg gggagggaaa ggaagatttt ctgtcctacg
     3181 gggatttgta ccgtaatgcc ctgaaagtct tatcctggtt gcaaagcaaa ggtctcaggc
     3241 agggaagcga gctggtattc caggtggagg acaacaggac tttcgtgatc gccttttggg
     3301 catgcctgac tgggggtctc atacctgttc cgttgactcc cggcaagact gccgaccatc
     3361 ggcaaaaatt gttcggtgtt tggtccctct tgcggcaacc tgcgttgctg atatcgcgca
     3421 acatgttgtc tcagatcggg gagtttgcga cactgaaagg ccttgaggcg ctatacgcgg
     3481 acatgatggc ggggatgatc gcggaagaag agctgttctc cggcacggaa gatgggcagc
     3541 cggtcgatgt ccgggaggaa gatattgcct ttatacagtt ttcttcgggc tctacgggta
     3601 accccaaagg ggtaatactg acgcaccgga atctccttac gaatatccgc tccatcggca
     3661 aagccgcgag ctatgccatg gacgatgtcg tgctcagctg gatgcccctg acccacgaca
     3721 tgggccttat tgggttccat ctcaatccct tattcgtggg tatgaatcaa ttcctgatgc
     3781 ctgcgggtct gttcgtccgg agacctgccc tatggctcga caaggctacc gagcataagg
     3841 ctacgataat gagctcgccc aacttcggat atgagtatat cgtcaaacat tgtctgaaag
     3901 agggagcatg gttcgattgg gacctgtcgg ccgtacgctt gttgtacaat ggcgccgaac
     3961 ctgtcagtga gaagatctgc cggcattttc tcgatacctt ccatgtgtat ggtcttcgcc
     4021 gcaacagtat gtgccctgtc tatgggttgg cagaggccag tgtcgccgtg accatttcgg
     4081 agccggcgga ggaggtaaaa gcagtggtcg tatcccggca tcacctgaaa ttgggagaca
     4141 acgttaccga accggaaagc gaggccgatg aggtcagttt tgtcaacgtc ggcaagccca
     4201 tcgacgattg tcagctacgg atcatggacg acgatgaccg tgaagtcgat cccggtgtta
     4261 taggccacat ccagataagg gggggcaacg tgaccagggg ctattacaac aatgacgccg
     4321 agaccaggag ggtcctcacc gaggacggct ggctgcgaac cggggacctg ggattcctgc
     4381 gacaagcctc tctctatgtc accggcagat ggaaggatat cttctttatc aacgggcaaa
     4441 actattaccc tcatgacatc gaaagcatcg cctgtgaagt agatggtgtg gagctcaata
     4501 aaattgtcat tgtaggcaga cgcgatgacg cgaattcgcg ggaggaggct attgcattcg
     4561 tgcttcaccg gggagaaaca gccgatttcc ttcatgttgc cctcgctata cggaacacgg
     4621 tgaacaggga agcgggcatc gttttagaca agatactgcc tgtgaaagat attcccagga
     4681 ccaccagtgg aaaactacag cggtataaat tattgcagca actgatggac ggcgagctcg
     4741 aggaggcgga caggctgatc acccccctgc tttcatcatc cgggatcata ggaggtgcgg
     4801 tgccgcgcgg tggggaagaa gcggcggtac tggagctgtg gaaagagata tccggcaacc
     4861 cggacgccgg gccggacgac cgatttttcg aaaccggagg caatagcctc aaagcggtcg
     4921 agctgagcat ggccgtccta aaagtattcc aggtggagct gggacccgaa ttgatctatc
     4981 aaaaacagac cgcgaagctg ctagccgaag cgatccgcgc gggtgttaag aagccttata
     5041 ccgcgatacc cgtggggcca cagctggatc actatccctt gtcgggggca caggaaagac
     5101 tttattatat atggaaggct gccccgtctt ccattgcgta taacctgccg gttgcgttcc
     5161 ggttgcgggg caggatcgac acggataagc tcgaagcctg cattcggaaa atgatcctcc
     5221 ggcatgatgc ctggagatcc gtatttctgg aggaagccgg accgcagctg tcgatcaggg
     5281 aagacatgcc ttttgatctg tcctgcagga actgccatcc ggatgagctg gatgccacgc
     5341 tcaggtccct ggttcgtcct ttccacctgc tggagggacc gctgttcaga ataaccttgg
     5401 ttaagaccgg cgattcggag caagtgctgc tggcagattt ccaccatagc atttcggatg
     5461 gcatatccct ttatcggtgt gcggaggaat tgatgagcct ttattgcgac aggcagccgc
     5521 cggaattgcc ggtggggtac aaagattata tattatggga gaaaggggag gggcatatcc
     5581 gtccctacgc ggcacaacag gcattctggc tcgaagagct gaaagggccg ttgccggtac
     5641 tggagatgcc cattcattcg ttgcggccgc cggtatttca aacggaaggc gggaagatgg
     5701 agttccattt gccgggaaag ctggtctccg gattgcatgg ggtcgccctg cggcaacgct
     5761 gtacgatgca cgtgctgctg ctgacgttgt ataagatcct gttgtggaaa tataccgggc
     5821 aggaggacct tattgccggt gtccccgttg cgggaaggaa ccaccccgat ctgcgtcatc
     5881 tgcaagggat gtttgttaac aacctggtga tccgaagcca tgtcagggga gaagacaact
     5941 tcctgtcttt tttggaatta caacgggcac gcatatccgc aatattggcg aaccaggagt
     6001 atccgttcga taggcttcta agccaactgt cccttaaacg cgactcgagc aggaacccag
     6061 tgttcgacac catgttcaat tatcagaata tgggggaccc cgcacgggaa gcggggggga
     6121 taatcatgag ccggtattct tttgatccgg gcttcgccaa attcgatatt tcaatggatg
     6181 tattcgagca ggacaataca ctggtctacg gcatcgaata ttgtacccat ttgtttgaac
     6241 ggaaattcat tttggacctg gcccggcatt tccaacacat gaccgaacgg gtggcggaag
     6301 atccggacag aagcgtggga gaactggcgg cccttccgga tgaggaaaca gaacggtaca
     6361 tctatcgatg gaatgcgtcg ggggccgatt atccccgggc aatgacggtg caccaactgt
     6421 tcgaagagca ggccgggaaa accccggatc ggatcgcgat cgaatgggac ggtaaagaga
     6481 tcagctaccg ggaactcaac gagcgggcgg gatccgttgc ccgttggttg gctgatcagc
     6541 acttagggaa agaggagatc gtagcactgc atatgccgcg gtgttccgaa ctgatcgtgg
     6601 ccatgctggg gatatggaaa gccgggggcg catttctgcc tatcgagatg gaactgccgg
     6661 agcggcgcaa acaggttatc cttcaaaaaa gcgggtgcag actcgtcatt acggaaaacc
     6721 acctgccgga agagatccgg ccgcacgagc cgcccgttcc gacgcccgcg agcctggcgt
     6781 atgtgatcta tacttcggga actacgggcg agccgaaagg ggtcgccatc gaacatgcgt
     6841 ccctcgtcaa ttatatacac tgggcggccg gccaatacct gcagggtgag cgtcatgcct
     6901 ttccgctgtt tacttcgata gcattcgacc tgacgctgac ctccattttt gttccgttgg
     6961 tcaccggcaa ccggatcgtc atttacaatc aacgtcaggg agaggtcttg ttagaaagga
     7021 tcatcgcgga taacaaggtc gggataataa agcttactcc ttctcatctg cggctactga
     7081 gggacggctg ccgacggtcg gagggcagtc gtataaagcg gctgatcgtt ggaggagaaa
     7141 gattggagac gcaactggcg gcggcgataa cgcaattact gggaggggat gtcgaaatat
     7201 acaatgaata tggtcctacc gaaagtacgg taggctgtat gatccatcgg taccgcagtg
     7261 aagaaaagcg cataggcgtt cctatcggca tacccatcgc caatacacgg atatacctgc
     7321 tggacgggca acaccggccc gtaccggaag gggtaaaagg agaactctat attgccggcc
     7381 gggggctcgc caggggatat ctccatgacg agggtcagga gaagttcatg gatgatccat
     7441 ttttcaaggg agagaagatg tacaggacgg gggatttggc catccggctg ccggaagggt
     7501 acctggatta tatgggacga tgcgatcggc aggtgaagat caacggatac cgtatcgaaa
     7561 ccggagagat tgccggagtg ctctccggtc tgccggaaat aggggatgtc gaagtgtcgg
     7621 taagaaaggg tgaggaggga aaagagaacc tgtatgccta ctataccctg accgataacc
     7681 ggcaggaagc gctgccggag acctggttgc gggattacct ggcggaacga ataccttatt
     7741 atatgatacc ggcgcggttt atcctgctga aaaaaatgcc gctgaacggg aatggtaaaa
     7801 tcgatatgga cgcattgccc ttacccgaaa cacaactccg tgagcaacgc cggcctatcg
     7861 ggacctggga agaattgtcc gtgaaaatat gggaggaaat tcttggacgg gaaggcatcg
     7921 gcgtccggga tagctttttc gagctggggg gtgactcgat aaaggcggtg cagatcgtcg
     7981 ggcggatggc agatgccggg gtgtcattac aggtacggga catcctcacc tatcacagta
     8041 tcgaacagac ctgccggcat gcacatgcgg tgcagggacg ttggtaccac cagggcaggg
     8101 ccgagggaga aaaagagcga tcgcctatcg aaacctggtt tttcgatctt gcgttgaaag
     8161 atccttcctt ctataatcaa tcagtgttgt tccgtctggg ccggcggatc ccaaaagcgt
     8221 tattggaaga ggcattccgc ttgctcatca accatcatga tacgctgcgg atgaatgtcg
     8281 acgtgaagac cggccggctt ttctacaata acaggcatgc ggaaacggaa ttttccatcg
     8341 gagaaggcgg aagtacggcc gatctccttc ctcttgccgg agggttcgac ctcggcagca
     8401 gtcttctttt gcgcgccagt ctcctcgcag acacagacgc gggaaactac ttatcgatca
     8461 ccgctcatca tctgatcatg gacggtgtct cctggcggat attcctggaa gacctttatt
     8521 ccgtctctac ggcgctggag aagggggagg agccgaagtt gccgcggaag acggcttcgc
     8581 tgaaagactg gcaggcggaa tggcggcgac acgccgatac ccttcttccg gaagaagaag
     8641 cctattggac ccgggtagaa gagataaggt ttgcacttcc ggtggacctg cagacaaatg
     8701 actggagggc gaagcacctg gcgacgcaat cgggccggct tagtccggaa gagacccggt
     8761 tattgctgca agcgcatgcc gtctatcggg cggacatttc cactatcttg ttgactgcac
     8821 tcgcggcggt ccttaaggaa tggacgggca gcgaccattg cgtattagag atggaacatc
     8881 acggacggga aatggagttc gcagatgtct cgcgtacgct gggatggttc acctcgctgt
     8941 atcccttgca cctccggtgt ccggaaggaa cagaagaccg tcgggtcagt gccatttggc
     9001 agcaagtgaa ggaggttccc aggcagggga tagggtatgg cgccgggccc cgcaaaagac
     9061 gaaagggtag cgaggactgc gccccgcgga cggagatcag gttcaattac ctgggccggt
     9121 tcgacaccga gttcgataac gaccttttcc aatacatacc ctcttccggc ggcatcggga
     9181 cagatccgga aaacagcatg acagcaaaac tggagatgat cgcgatgatc tttggcgggc
     9241 aattgatcgt cgacgtgcat tataaccggc tggcgcatga ggatggcacc atccggactt
     9301 ttatggaagg gtttctccgc catctgcgcc ggctggtggg gccaacagac aagaacgacc
     9361 aggccgggga gttcctgcct tccgactttg gtgcagttga actgaatgag aaggattgga
     9421 agacgctatt cgggtaattg atcatcgaaa gatcaaatga atcgacaatg aatatggttg
     9481 cagtcgttgg tgcaggcgtc atgggcgccg atgtggcgct gaccctttcc tgttacgggt
     9541 accccgtagt tttaaaggat atccggcctg atgccctgca acaggccagg gaaaggattt
     9601 gccgcgacct ccggatggtc aggatgatga aacccgtatt taaaaacctg gcggccgaag
     9661 aggtgatggg aaggatccgt tttgcactgg attatgaggg tttcgagagg gcggatatcg
     9721 ttatcgaaaa tgtctccgaa acatggacgg taaaggccgg agtttaccag gaactgaaag
     9781 gggtctgcca cgggaatgta ctggtttgtg tgaataccag ttgtatttcc attacccgga
     9841 tagcggcctt gctggatcat ccgcaaaatg tcatcggcat gcatttcatg aacccggtgc
     9901 cgttaaagga gattgtcgag gtcgtaaagg gctatcatac gtcaccggat accatcgaca
     9961 gggcgaaagc ctttctgcgc tgtttggaaa agacgccggt gctggtgaat gatctgccgg
    10021 ggttcgtggc caacaggcta tcccatctgt tcatgaatga agcggccttc ctgatccagg
    10081 accaggtggc atcggcccgc gaagttgatc ttatcttcaa aaaaggttat ggccattcga
    10141 tggggccatt ggaaacggcc gacctgatcg ggctcgatac ggtggtcagt tcgctggaag
    10201 tgttgtatac cagctaccag gacccgaagt tcaggtgctg cccgctgttg aggaaaatgg
    10261 tagatgccgg attgctgggc aaaaaatcgg gtgaagggtt cttcaaatac caggacgaat
    10321 gacccatctt aaagaagtga agtgcgtggt gtgggatttg gacaacacgc tatggtgcgg
    10381 cacgctgctg gagccgggcg acgtcttgct cagaccgggc atcagggagg tgatcgccga
    10441 actggatgga agagggatac tgcattccat cgccagcagg aataactacc aggacgccat
    10501 gggaaagctc cgggagtggg acctgcacga tttctttctt tatccccaga taaactgggg
    10561 ttccaaatcg gaatccatcc gattgatcca ggagaaactg aatatcggca tggacaccat
    10621 cgtattcatc gacgatcagc cgttcgaaag agatgaggtg tcggaaagtc tcccggaggt
    10681 agagacattc gatgcttcga cttacctcga cctgcccgcg ctgacgagga tgaatccccg
    10741 ttttgtgacc cgggacagca agcagcggcg gatcatgtac caggaggata tgcaaagggt
    10801 aaaggatgaa gaggaatata tcggaccgcg ggagtccttc atggcatctc ttcgaatgcg
    10861 gttcattatc tccgaggcgg aggaggaaga cctcgaacgt gcggaggagc ttacggtaag
    10921 aacgaaccag ctgaacgcca ccggtcggac atacagttat gaagagctgt atgcgctcat
    10981 gaagtcggat catcataagc tgtacgtttg cgagttgact gatcggtatg ggtcctatgg
    11041 gaaaataggt ctggcattgg tggagatgca ggcggaccat tggtgtttga atatgctgct
    11101 gatgtcctgc cgggtaatgt cgaggagcgt cggctcgatc ctgcttagct atatcatgca
    11161 acgggccagg caggacggaa aaaagctact ggcgcatttc aagcaaacgg accggaaccg
    11221 gatgatgtat gtcacctatc gtttttccaa cttttcggaa ttgaccaacg atggtcatgg
    11281 gaacatcgtc ttcgcaaacg acctcagcca ggtccagggt tttcctcctt atgtggaggt
    11341 gatgactccc ggggccgccg gctttcccca tagccaagct taaacgacag cagcatgaca
    11401 ataagagaaa agatcaggag cttcatcggg gccaatttgc tggtatccgg cgaagacgag
    11461 gaattctccg atgcagacaa tctgttccag ctgggatatg tcaattccct gtttgcgatg
    11521 aaattgttga actatgtgga acgggagttc gacataacgg tggacaacga agcaatggat
    11581 atccgaaatt tcagctcggt tctcaatatc gtgagcctgg tggaaaaaat cagacaaacg
    11641 tgacggactc tttacaggac aggatactcg ccgaggcaaa ggattttgcc gaccgggaga
    11701 taaggccctt tgctgctgtc ttcgacaaga accagggtat atcccgggat ttgatcggaa
    11761 agatggccga acgcggctac ctggctccct gtctgccccg gctatatggg gggctggagc
    11821 tggatgcgtt gcattacgga ctttttaccg agatcatcgg gaaagcctgt ccttcaacgc
    11881 gtgtattgct gaccgtgcac tgctcgctca tcggtgagac catcctgcga tggggtacca
    11941 tggaacagaa gaaccggatc ctgccggaac ttgctggtgg ccggcggctg gctgcgtttg
    12001 cgctcaccga gccggggacg gggtcggatg ccaaaaatgt gcagacgctc taccggcagc
    12061 agggagacca ctatcatatc acaggaaaga agaaattcat ctccctggga gcaatcgccg
    12121 atctcttcct tgtgctggcc cgtggcgccg caggcgaggt aacggccttt ctcatcgaca
    12181 ggaacagtcc cggcatttcc atcgagccta tgaccggatt gctggccagc agggctgcct
    12241 atgtagcagc aatcgggttt tccgatgtct gcgtgccggc agaaaatgta ttgggaaaag
    12301 agggcggcgg gttcacgttc atcgtcaata cggcgctgga ctatggccgt tatagcatcg
    12361 cgtggggcgg tctggccatc gcgcaggagt cattggacgt gatggtggct tatgccagga
    12421 acaggaagca atccggtcgt cccatccata ccttccaatt gatagagggg atcatcggcg
    12481 atggcgtggc ccgggtggag gccgcccggt cgctgtgcat gcatgctgcc agattgaggg
    12541 accagcggca tgaggacgcg gtcatccaca ccactatcgc caaatatttt gcttccaggg
    12601 cggccaaccg gctcgcttcg gacgcggtgc aggtgcacgg aggcaatgga tgctacgatg
    12661 agtatcctgc cgagcggcta ttccgcgagg cgaaagtgct ggagatcatc gaggggacct
    12721 cacaagtcct gcaggcggtg atcgccgaat acggactaag gaagttctat gccgggccat
    12781 ctataaaatc aacatcgttg tgaaagcgaa aatcgaaaag agcaatgtcc tggagatatt
    12841 cgagcttagc tccatccaaa agggcatcct cttccactat ttaagagaca gcggttcaca
    12901 catatacaac gtccagctcg ttttcgacat ccgcggttcg ctggataaag accggctgga
    12961 ccgcgcggtg gcagccgtac agcgccaaaa cccggtgttg aggtccgtct tcagctggga
    13021 aaaggtgagc aagccgctcc agatattgct caaaacctac ccgcggccat tcgaatacat
    13081 cgatatttcg gggatcggga cgcaggaaag acaagccagt cttcagcgat gcctggaaga
    13141 ggaccggcaa gagcgattcg acctggagac cctggcgttg cgtctaaagc tgataaagac
    13201 ctcccccgat acgttcgttt tttcgatcac ccatcaccat atcctttacg acggctggag
    13261 tacgggttta ctgctgacag aactgttcct ggcttaccgg catccggatc gtgagccgat
    13321 aaagcccgat tatcccggaa tataccggcg ccatgccgga cgaaacctcc cggaggatgg
    13381 ccggcgttat tggagagcct atttggaaga ctatcagatg cattcgttgt tgccacccat
    13441 ccgggatcga aaccatacgg taaatagctt tcgggtggga aggcctatgg cagccctcga
    13501 tcgtttctcg gcgcaacacc gggtgacgaa ggcggcgctc atctatgcgg cctttggtct
    13561 attgctgcag aaatatgcca acagcagtga tgtagtgttc ggaacgcccg ttgcggacag
    13621 ggatcccgcc attccgggca tcgaacggct gatcggcaat tttatcaata cgttgcctct
    13681 acgtgtgact gcgacagacg aagcgacgct cctggatatc gtccttcgtg tacatgcgga
    13741 tatggctgac cgtcatgaac acagccacca ttcttatgca gagatcaagg atgtgttgaa
    13801 actgcgcccc gatgagaacc tgttcgagat tacggtcgca gtggagaact atccattgga
    13861 caagaccgct atcgaaggga ttgaaggtct ttccgtcact ttgcgctcca gctatgaatt
    13921 tacggacagc ccgatagccg taaacgtctt tttcggcgcc gacctgggag tgagcattgc
    13981 ctatgacctg gcatttgccg acgaggcttt cgcgcgacaa ctcgcggaac atttcctggc
    14041 catcctggat aagatctgcg acgacccttt ttgcctcgtc catcggctgg atattctttc
    14101 cgatgccgaa aggcggctgc tcctggagac attcaacgat acggccgccg aattccccga
    14161 aaccgaaacg atcgtctctc tcttcgaaaa acaggtgtcg cgcacgccgg acaatacggc
    14221 catttgctgc gacgggaaga ttttttccta ccgcaccgta tatgaagaga tgaatcgtat
    14281 cgcggctggc ctcaccacaa cctatgcatt gggagcggga gacgtcgccg gggtgctcat
    14341 gatgaaatcg gagcgcctcc tcatcaccat cctggccatt ctgaagaccg gcgcttccta
    14401 tttacccatc gatctgagca atccgcctga ccgtgtcgcc tatatggtgg aggacagcga
    14461 tatgaagcta ctgatcaccg atcagccatc ggtcagtgcg attacaccgt cgggattatc
    14521 ggtactcggg tacgagacat tgcaggcggc ggccggatgg tccgggaaca tgaccggggc
    14581 accttgctgg cccgattacc tggcctatac tatctatact tccgggtcca cgggtatgcc
    14641 caaaggcgta ggtctcaaac accgcggttt tgtgaacctg atctactggt atgcaaaggc
    14701 gttggccggc acagagacgg acaattatct cctcatcgct cccgtaagct tcgacctggc
    14761 gcaaaaaaat atttttaccc ctttgctcac aggggcctcg ataaccttgt cggaggatat
    14821 tcaattggac tatggccgca tggcggcgac gatcgcgtcc aaaggcgtga cggtcatcaa
    14881 ttgtgcgcct tccgcctggt acccattgct ggaggaagct gcgcacaacg gctaccgcga
    14941 cctggccggg gtacggaaag tcgtattggg tggggagaat atccatatga aagccttcga
    15001 gggctggttt cattccggca acttccatgc tcagctgatc aactcctacg gaccaacgga
    15061 atgtacggat gtagtcgcct ggcaccaatt ggaagatccc gtgcatcgtg cctatgacag
    15121 ggtgccgatc ggcaggccgg tcgacaatat gcgactctat attctcgacc ggcagggcgc
    15181 cttacagcca ttgggggcgc tgggcgagat ctgcatcggc gggacaggcg tttccgacgg
    15241 ctatcggaac aacccggaac tgacgcggag gaagttcttt cccgatgcca tttcgggaaa
    15301 ggggatgtta taccggacag gggatgcggg caggtggctg cggaatggtg aaatggagtt
    15361 cataggacgg atcgacaacc aggttaagat aaggggtttc cgggtagagc cgggggagat
    15421 agaagtacaa ctgagccgtc atgagcgggt gcggcaatgt gccgtcatcg tgggcgaaaa
    15481 ggaggccgaa aaattccttg tggcttatta cgtggccgac catccgctgg agagtgcggc
    15541 gttaagagca ttcctctccg ccaggttgcc gcattatatg gtccctgcct ggtatgttca
    15601 cctgacatcg ctgccgagga ctcccaacgg caagctcgat cgaagggctt tgccttctcc
    15661 gcagattcgc gcagagagcg agcagaccgt tcctacgcgg gcaatggaga agaaattact
    15721 ggcgatatgg tgcgatgtgc ttggacaaac gcggatcgga gtcacggaca atttctttga
    15781 tgtaggggga gattcgttga agctgataac cgtgagcagc cgtataagag tggatatgga
    15841 aaagcaggtg ccggtgaccg agctgttgca atatcctacc atcaggacgc tggccgacca
    15901 cctgcagcct gccgtccaaa agaaccggga acagtcgaac gccgaaccgt ctgcggcagc
    15961 gaaggacatc gcgatcatcg ggatggcatg ccggtttccc ggggctcccg atgtcgatgc
    16021 tttctggggc aatctgcgcg cgggccggga atccattacg agggagggag atgcggacat
    16081 ctccggagag taccggctgg tgcgcgcgaa gggtttactg gagggatatg agtgtttcga
    16141 cgccgctttt tttaattaca gccccgcaga tgcaaagctg atggaccccc agatacgcat
    16201 ctttcacgag acggtctggg aggcgctgga gcatgcaggt tatcgctgtc cttcatttcc
    16261 cgggaggatc gggctttatg cgggggctac tcccagtccc tattatgaac tggataccag
    16321 caagccgcag ggggaccaac tgctcgagga gtgggcctcg ctaagttatg ccgacaagga
    16381 tttcctatgt gccagggttt cctataagct gaatctacgg ggtccgagcc tcaatatcaa
    16441 tacggcctgc agcacttcgc tggtggcgac cgacctcgcg tgccaggagc tgatggcagg
    16501 caaatgcgag atggctgtgg cagggggtat ctctgtgacg ctgcacgaca acgaaggtta
    16561 tatctatcat aaggggatgg tcatgtcccc ggacggacgg tgcagggctt tcgatgcgga
    16621 agccgcgggg acggtgggcg gcaatggttc gggagccgtc gtactccgaa gattggaaga
    16681 tgctttaagg gatggggaca cgatttacgc ggtgatcaag ggtacggcca ctaacaatga
    16741 cggaaatcgt aaggtcggtt ttaccgcgcc tggtgtcgat gggcaggcgg cggtgatcag
    16801 ccacgcgatg aggaaggcgg gcgttcgtcc agaggatatc ggctatgtgg aagcgcatgg
    16861 gtcggggacc ttgttgggcg atcccatcga ggtggccgcg ctcgtcgagg cattcggcac
    16921 ggacaaacgc gcttattgcg gtctcggttc ggtgaagacc aatatcggtc acctggatgc
    16981 cgcggcgggt atcgccgggc tcatcaagac agtattggcc ttgtaccatg gagaaatacc
    17041 gcccagcctt catttccatc agcccaatcc gaacctagaa cttggcgcga gtccttttta
    17101 tatcaacgat cgactacggg attggcaaaa ggacggtagg ccccggacag caggcgtaag
    17161 ctcattcggg atcgggggca ccaacgcgca tataatcctg caggaagctc cgccgaggtc
    17221 cgcgggctcc gcgagccggt cgcaccaact tttgttgcta tcggccagga gcgagaatgc
    17281 gcttgccgcc aactccgccc gtttggcgga tcacctgcag gcaatggccg acgaggagca
    17341 ttccggccgg acggcagagc agcaggcgct tgcagatctg gcatataccc tgcagacggg
    17401 cagagagccg atgccttacc ggcggatgat cgtctgcgat acggtggtgc aggctatcga
    17461 acagctgagc atattcccgg tgaatggaaa gactagtccg ctggatgaaa gtctggcaaa
    17521 ccgcgtggtc ttcctgttcc ccggtcaggg tgcccaatat tcgggcatgt gccggggcct
    17581 gtacgaaaaa gaaccttatt tccgggaaca gatggacagc tgtcttgcca tcgtccggca
    17641 attgtccggc aaggacctga agccgctgat cttcggggga tcgcgaaggc tccacgacac
    17701 cgagacagcg cagcctgctc tttttgtcgt ggaatatgcg cttgcccggc tgttgatgaa
    17761 atggggcatc ctgccacacc tgatgatcgg acatagcttt ggggaatatg tggctgcctg
    17821 tctgagtggg gtcttttccc tggaggatgc actatccatc gtcgtaaaaa ggggagagct
    17881 catgcaacaa gcgccgggag gcatatcgtt gggcgtagcc gtttcagaag aggtcctgac
    17941 ctcctggctt gaaaaatatc ccggtgtatc gcttgcgacc gtcaacagct cggagctttg
    18001 cgtggtttcc gggcaggcgg aacaaataga ggcattccgg aaagaaatgg ttatttgcgg
    18061 ttatccatcg gaggtagtca gcgaggcgca cgccttccat tccagccgga tggatgggat
    18121 attggaagcg tttgcatcgc atatacggca gatccatccg gcaacgccgc gtataccgtt
    18181 tgtatccaac ctcacagggg aggtagcaac ggcagatgcg gtgacgggta ctgcctactg
    18241 ggtcgaccat ttgagaaaga ccgttcggtt tgcagatggg atccgcaccc tgatgaagga
    18301 ggagcatatg gtattcgtcg aagtggggcc cggaatagag ctgagcagct ttgtgagaag
    18361 catgcgggag agaggtccgg ggcataaggt gatcaatatg gtccgccatg cgcagacaga
    18421 aggggacgac cagtatcatt tggaaaaagg gatcggcaga ttgtggctca tcgggatcga
    18481 gccggcatgg ggcagctttt acgagggcga gacccgccgg cgggtgccct tgccatccta
    18541 tgctttcgac cgggtttgct tccccgtgag gaacaagcga cccgtcgccg gtacggcaaa
    18601 gacggaagaa gaccttgtca gatcaaaaga catatcgcga tggtggtatg ctcccacgtg
    18661 gcgtcatgtg cccctgctcc cggagccggc cggcagcgat ggatcgtgca cgttgttgct
    18721 cgacgatgac ttatggatcg gcgcgggcgt ggcggaagtt ttgcgcggtt atggagaacg
    18781 ggtcatcctg gtaagaaagg ctgccgcgta taagaatgac ggggatc
//
LOCUS       DASYSV010000079         3862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_16984, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000079 DASYSV010000000
VERSION     DASYSV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16984"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /gene="ychF"
                     /locus_tag="VGQ51_02420"
     CDS             complement(<1..752)
                     /gene="ychF"
                     /locus_tag="VGQ51_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV9035440.1"
                     /translation="MALQAGIVGLPNVGKSTLFNAVSNSAKAQASNYRFCTIEPNTGL
                     VDVPDERLDRLAALVRPQRIVPTQVAIVDIAGLVRGASKGEGLGNKFLANIREVDAII
                     HVIRCFEDENILRDEGPIDPVSDKEIIDTELQLKDLESVEKKLNRTEKMLKTGDPKIK
                     AEYEVLKRCYQTLEQGLSIRTLGLSKEERTAIDDLFLLTEKPVLYVANVDEASMHAGN
                     KFSETLKTAVAGENAQVIVMNNSIEAQIAEME"
     gene            828..2093
                     /locus_tag="VGQ51_02425"
     CDS             828..2093
                     /locus_tag="VGQ51_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEV9035441.1"
                     /translation="MSLSRIWAAFILVSITVAVARYAFVPADKAIFSQMVTGKATDPG
                     ARADGLIETCKTAVNLAIGLIGIVALFMGLVNIAEQAGGIRLLARWSAPFFSKLFPEV
                     PDGHPAMGHMVMNFSANMLGLDNAATPFALRAMESLQELNPDKTAASNSQIMFLALHA
                     SGLQIIPVSIIALRAASHAPDPTDIFIPIVIATFITTVASMFIVAAWQRINIFKPVVL
                     VWIGSISVIIAALVIYLRSLDPDHVQSLTSALSGGLILAIFLCIIAGALYKKIDIFAA
                     FVEGAKGGFDIAVRIIPYLVGMLAAISLLRTSGTFDTVLGGVRHLFGWLGTDTRFVDG
                     LPTALIKPISGAGARGMMVDAMQHDPKGFAGRLSGILQGSSETTFYVVAVYFGAVGIK
                     NTRYSIPAMLLAELVSVIVSIALAYLFFG"
     gene            2367..3443
                     /locus_tag="VGQ51_02430"
     CDS             2367..3443
                     /locus_tag="VGQ51_02430"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9035442.1"
                     /translation="MKASSLKRVSLHVLFWLAYLTEDVTLIYLWDKDLLTKISPGHQL
                     GLAILNSLTSLIPKLLFIYYVLYYNLPKLLNAKTERRKYIVLLFLALIVTISLYRVIG
                     VYFQGPILYHGLAQYGPLFSGRRFLQFLMDVGFPAGLAIMIKQFRLQLAAKEKEQRLI
                     RDKLETELKYLRNQTNPHFLFNTLNNIYALARKKSDETADVVMKLSKLLRFMLYETRN
                     AQIRISDEIKTLDDYIELEKLRYNDRLTLSFVREIDNDNEPIAPLLLIPFVENAFKHG
                     ASESRFESFINLELVLQNGILEFYIENTKEENGKQTNDENIGLSNVRRQLELLYREHE
                     VSIKCEPTVFKVFLKINLRRYANL"
     gene            3430..>3862
                     /locus_tag="VGQ51_02435"
     CDS             3430..>3862
                     /locus_tag="VGQ51_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9035443.1"
                     /translation="MQTYNCIIVEDEPLAAEVLQDYIRQVPFLNLVGCCSDAFFAMES
                     LQKFDVDLLFLDIHLPKMKGLDLITVLKKPPKIIITSAYQEYALQGYELNVIDYLLKP
                     IEFSRFLKAVNKMEQTREMALPVRSPVLPAERVYLFFIVGKK"
BASE COUNT          879 a          919 c         1055 g         1009 t
ORIGIN      
        1 tcttccattt cggcgatctg cgcctcgatg gaattattca tgacgatgac ctgcgcattc
       61 tcgccggcga ccgcggtctt cagcgtctcg gagaacttat tgccggcgtg catcgaggct
      121 tcgtcgacgt tggcgacata caagaccggt ttttcggtca gcaggaaaag atcgtcgata
      181 gcggtgcgtt cctctttgga gaggcccagg gtacggatgc tcagcccttg ttcaagcgtc
      241 tgataacagc gtttgagcac ttcgtactcg gcttttattt tgggatcgcc ggtctttagc
      301 atcttctcgg tgcggttcag ctttttctcg acgctttcca ggtctttgag ctggagctcg
      361 gtatcgatga tctctttatc actgacggga tcgatgggtc cttcgtcgcg aaggatgttc
      421 tcgtcttcga aacaacggat gacatggatg atggcatcca cctcgcgtat attggccagg
      481 aatttattcc cgagaccctc acctttgctg gcaccgcgaa cgagaccggc gatatcgacg
      541 atggctacct gcgtaggtac gatgcgttgc ggcctgacca atgcagcgag acggtcgaga
      601 cgttcatcgg gtacatccac aagcccggta ttgggttcaa tcgtgcagaa ccggtagttg
      661 ctggcctgtg ccttggcgct gttactgacg gcgttgaaaa gagttgattt ccccacgttc
      721 ggtagtccca cgatgcctgc ttgtaatgcc ataattttcc ctttgaggcc gcaatattag
      781 gcaattaatg gtaatttgag ggcgttaagt gtaaataacg gattattatg tcattaagca
      841 ggatttgggc ggcttttatc ctcgtctcca taaccgtcgc ggtagcgcga tatgcgttcg
      901 tacccgcgga caaagcgatc ttctcgcaaa tggtgacggg taaggctacg gatccggggg
      961 caagggcgga cggtttgatc gaaacctgca agactgccgt aaacctggcg atcgggctca
     1021 tcggcattgt cgcattgttc atggggctgg taaatatcgc ggagcaggcc ggcggtatcc
     1081 ggttgctggc gcgctggtcg gcgccttttt tttctaaatt atttccagag gtgccggatg
     1141 ggcatccggc gatggggcat atggtcatga atttttcggc gaatatgctt ggactcgaca
     1201 atgcggcgac gccattcgcg ctccgggcga tggagagttt gcaggagttg aaccccgata
     1261 aaacggcggc ttccaacagc cagatcatgt tccttgcgtt gcatgcttcg ggtttgcaga
     1321 tcatacccgt gagcattatc gccctgcggg ccgcttcgca tgcgcctgac ccgacggata
     1381 tctttatccc catcgtcatc gccaccttca tcacgacggt tgcatcgatg ttcatcgtgg
     1441 cggcatggca gcggataaat attttcaagc cggtggtgct ggtttggatc ggaagtatct
     1501 cggtgatcat cgccgcactg gtgatctatc tgcggagcct cgatccggat cacgtacagt
     1561 ccctgaccag cgccctcagc gggggactga tcctggcgat ctttttgtgt atcatcgcgg
     1621 gggccctgta taagaagatc gatattttcg cggcttttgt ggagggtgcg aagggtggat
     1681 tcgacatcgc cgtgcgtatc attccttatt tggtgggtat gttggccgca atcagcctgt
     1741 tgcgcaccag cggcaccttc gatacggtat tgggaggggt gaggcatctt ttcgggtggc
     1801 tgggcaccga cacccgattc gtagacggtc tgccgactgc attgatcaaa cccatttccg
     1861 gggcgggggc acggggaatg atggtggatg cgatgcagca tgatccgaaa ggttttgcag
     1921 ggcggctctc ggggatattg cagggctcgt cggagactac attctatgtg gtggcggtgt
     1981 attttggggc ggtagggatc aagaatacgc gttattccat tcccgcgatg ctgctggcag
     2041 aattggtgag tgtgatcgtg agtatcgcac tggcgtatct tttctttgga taaaaaagcc
     2101 cccgcgtgag gacgggggcg tcgattgcaa acccagacct atgactcaat gctgacaaaa
     2161 aattggtttt ggtgtaccgg ccatcggcca ggaaattaaa ttagcacatt atcccgccaa
     2221 atccatttaa tttcaatgaa catcggggtt gaccccacag acagcagcat tcaacctacg
     2281 atcgccatac tgtcccgaac tttagcggcc gattcctgca tcatccttcc ggaaatgccg
     2341 tattttgttc ttcctggaag cgaaaaatga aagcaagtag tctaaaaagg gtatccctgc
     2401 atgtcttgtt ctggctcgcc tatttgacgg aggacgtaac cctcatctat ctgtgggata
     2461 aggatctgct cacgaaaatt tcgcccggcc accagctggg actggctatt ttgaatagtc
     2521 tgacgtcgct gatcccgaaa ttgctcttta tctactatgt cctgtattac aacctgccga
     2581 agcttttaaa cgcgaagacc gaacgaagaa aatatatcgt cctgctgttc cttgccctga
     2641 tcgtcaccat ttctttatat agagtcatcg gggtatattt tcagggtccc atactctatc
     2701 acggattggc gcagtatggt cctctgttca gcggccgccg gtttttacag tttttgatgg
     2761 atgttggttt tccggccgga ctggcgatca tgatcaagca attccggttg cagctggcgg
     2821 ccaaggaaaa ggagcagcgc ctgatcaggg ataaactgga gacagagttg aaatatctca
     2881 ggaaccagac caatccccac tttcttttta acacattgaa caatatctat gcattggcgc
     2941 gaaagaagtc cgatgaaacc gcggatgtgg tgatgaaact gtcgaaactt ctgcgattca
     3001 tgctctatga aaccaggaat gcgcagatca ggatcagcga tgagatcaaa accctggatg
     3061 attatatcga gttggagaag ctaaggtata atgaccggct tacgctgagt tttgtcaggg
     3121 agatcgacaa tgacaatgaa ccgatagccc cgcttctttt gatccctttc gtggaaaatg
     3181 cattcaagca tggggcaagc gaaagccggt tcgaatcctt catcaatctt gaattggtgc
     3241 ttcaaaacgg gatcctggaa ttttatatcg aaaataccaa ggaggagaat ggcaagcaaa
     3301 ccaacgatga aaatataggg cttagcaatg tccgcagaca attggagcta ctgtacagag
     3361 agcatgaggt ctccataaaa tgtgagccca ccgtttttaa agtgttcctg aaaattaacc
     3421 tgaggcgata tgcaaaccta taactgtatc atcgtggaag acgagccatt ggctgccgag
     3481 gtgttgcagg actatataag gcaagtgccc tttttgaacc ttgtcggatg ctgcagcgac
     3541 gctttttttg ccatggagag cctgcagaaa ttcgatgtcg acctcctgtt cctggacatt
     3601 catttgccaa agatgaaagg gctcgatctg atcacggtcc tgaagaaacc gccgaagatc
     3661 atcattactt cggcttacca ggagtatgcg ctgcagggtt atgagctcaa tgtgatcgat
     3721 tatcttttga agccgataga gttcagcaga tttttaaagg cagtcaataa gatggagcaa
     3781 accagggaaa tggcgttgcc ggtcaggtcc ccggtcctgc cggccgagcg cgtgtatctg
     3841 ttctttatcg tcggtaaaaa aa
//
LOCUS       DASYSV010000080         7261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2066369, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000080 DASYSV010000000
VERSION     DASYSV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066369"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(903..1127)
                     /locus_tag="VGQ51_02440"
     CDS             complement(903..1127)
                     /locus_tag="VGQ51_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035444.1"
                     /translation="MPDTTLHIRIKKEYAAALIEDLIKVNAVEAVDEDEDIELTAQQK
                     EALDKELVAIAANPAYLQRWDDVKRRFKKL"
     gene            1228..1839
                     /locus_tag="VGQ51_02445"
     CDS             1228..1839
                     /locus_tag="VGQ51_02445"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEV9035445.1"
                     /translation="MSIIRNALAGPLATALLTAGLLAACQNSPDNVKTAEKVNADRID
                     SANKTASLADSASVPTKQDAQFMVKAKSGNQLEVTLGELAQTNAQSLAVKRFGLMMVR
                     DHTEGEKELRSLAIAKRVVMPDTLSKQQQKEADDLKKKTGHDFDKAYIDLMVDDHKED
                     IDEFTKAAQQANDPDIKALAAKMVPVLKEHYDSARILKRNGGR"
     gene            complement(1908..2753)
                     /locus_tag="VGQ51_02450"
     CDS             complement(1908..2753)
                     /locus_tag="VGQ51_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV9035446.1"
                     /translation="MSTNVSFRKEFTLGDDLTVRRMGFGAMRITGQGIWGPPKDHDEA
                     IRVLRRAVELGVNFIDTADSYGPDVSEELIAEALYPYPKGLVIATKGGFLRSGPNQWH
                     INSSPDHLKEALEGSLARLRLDRIDVYQLHRIDPTVPFEETLSFLQGVQEEGLIKHVG
                     LSEVGVEDIKKAEEYVEVVSVQNKYSQDFRKWEPVLEYCEQTGKAFIPWNPLNAGKLE
                     NMTKIESVAGRLGVTPHQLALAWLLYHSPNNLLIPGTSSVRHLEENMDAGKIELNEEL
                     LEELS"
     gene            3464..5914
                     /locus_tag="VGQ51_02455"
     CDS             3464..5914
                     /locus_tag="VGQ51_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035447.1"
                     /translation="MFRNYWKIAIRSLLKRKGFTLINILGLATGMAVCLLILLFIRRE
                     TGYDNFEPAGDRIYRVVLERKYPGRSTSYAIIPQSIGGAIQKEFPEVQASTGVQDITG
                     NANFLVRIGDRNFEETRVLLADSLFFRVFPMHLLAGDTATALQQPNMAILNETTATKL
                     FGSAGAALGKTFESENKQHYQVSGVCADWPDNSHISFNILLSAASFPFTKVPNFTGFS
                     TYTYLLLRPNTDPAALEAKFPQIVQKYVAGEIGRSFGMSFAQFEASGNGYHYYMQPLG
                     QIHLISDLEAEMRPNGSKRAITIFGIIAIFILGIACVNFINLSTARSIERAREVGIRK
                     TFGSERRSLIGQFLLESTVVSILSVALAAGILTLALPWFNRLAGQQLSILAFLTPVNI
                     SGILAFGIAVGVLAGIYPAFVLSSFRPITVLKGRFQSARYGIVLRNALVVFQFAISII
                     LIICTVVVHQQMRFMLGDKLGFTRDAIVEIQRSDLLDKNTGAFRNALRSIPGVEEIGG
                     SSSMPGDANFFGIAWQQVGSTESMTGRGIVVDDQFANTLGLTLKEGRFFSKSYATDSL
                     SVVLNEKAVSALGLKEPVIGTRLTTTSGFLNPRPDSFYTYTVVGVVNDFHFQTLHQAI
                     APLVMTDAVKFGDATAITAVRLKGSEFSSAIASIGQQWKRFVPQRPFRYTFLDQQLAA
                     QYKAEQTLQRLFTVFSLLAIFIACIGLLGLAAYSTQQRIREISIRKVLGARPGSIIGL
                     LSVGFIRLVTVSAFVAFPLAWLAMHSWLQGFAYRVGLGWWVFVLAWLLSLVITLVTIS
                     LQAIRAAGVNPVKILRSE"
     gene            5925..>7261
                     /locus_tag="VGQ51_02460"
     CDS             5925..>7261
                     /locus_tag="VGQ51_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035448.1"
                     /translation="MIKNYLLTAWRNLVKHKLNASILVIGLTVAFTCCTLLFLMVRYE
                     FSYDRWHTKKDRLYEAYERNESPDGERRREGFPYPAARTMKAEIPSIVRSTSFMSAGG
                     AIRYRGKEVYQRLVLVDSDFFAMFSFPVVAGNGISPLANTDKVVINQTVATALFGKDE
                     AVGRHVQVKISGQWKALTVAAVLRDVPANSSLQFGILARIEIHKDYPTQKDDWGDSHH
                     RVYVELAPGATQQVAEAGLREMVHRHNLSDSDFLKSEGYLRDRNGDYLSIRLVRLPDV
                     HFDGVLGGRSSIDKSYLYTMMLIALIVMVIACFNFVNLNVARSFTRAREVGVRKTIGA
                     GRRDIYLQLWLESLILFVIALVLSMIASGSLLHPFNDLFTEKLRLSSLLDPAVLGIIA
                     GATVLISFLAGGYPGWMVSRFHVVDVLKGKVSMRRNSFLRSGLITLQFIMAAA"
BASE COUNT         1670 a         1920 c         1984 g         1687 t
ORIGIN      
        1 tgtgcctttt gtgacaatca tgcttttttt gtactatgtt ttcttggtat tgtttaagat
       61 tcccaagggc agggtgttcg gcatgttcta ctaggatcaa gagcttgcgt tgacccaagt
      121 tatgactcgc gaagactccg ccctgataag ccctaagtat attgatcgtg taaggtctca
      181 tcaaaacgtc aatagcaagc tgatggatcc ggtatcattt ctttatgtag acgatactta
      241 ccatttgatc atatttaaaa tgcatatggg aactgatcga cctttgaagc agatattaag
      301 gtgtgatatt gagagttcgc aacgatcgaa cgacgaaacc tattctatgg tagattttaa
      361 cggattttcc gggtttgagt ggcggccggt gcctatgatg gagggcagga atatcttttt
      421 atctcttgta agaatttttc cataaaatat gagaaagaag gggtagtaca ggtctttatg
      481 gcgtgtggag tgaacacacc ttcggacatt ttctttttga aaaggggtaa gatcgtatac
      541 tttatgctga tgaccactgc taaacccgat tctattattg attccggttg caaaatgttt
      601 cgttcactca tgcaataatt tacccggatc cagataagta ttgaacacgc gaaggatgtt
      661 gatagagtgc ccggcttcat cgatggtgaa ggtgataaga aatgggaagc gtttcatggg
      721 tttgcagcgg acggttttat agcggacgga agaggcggtc ggcaggtcgg caatacgatc
      781 aaaatactcg gatagcaggc cagcgaaccg gtaaccaaga tcaaccgcaa cggcattata
      841 ctattccacg gccacggcga tatcctcgat agcagcagga gtgacaaaaa tcgtgtaagg
      901 cattacagct ttttaaagcg gcgtttgaca tcgtcccacc tttgtaagta ggcggggttg
      961 gcggcaatag caaccagttc tttatcgagc gcttcttttt gctgggccgt cagttcaata
     1021 tcctcgtctt catcgacagc ttctaccgca ttgactttga tgaggtcttc gatcaaggcg
     1081 gcggcatatt cttttttaat gcggatatgt aatgtagtat caggcataag aaagtagcat
     1141 acaaatttaa gctatttatt cctgcttttc aaaccaaaaa agctggcctc gggcacacct
     1201 tttgaaccgc ccttccaaaa aacgaacatg agtataatac gtaacgccct cgccgggcca
     1261 ctggcgaccg cactgctgac cgcgggcctg ctcgccgcct gccagaattc cccggacaat
     1321 gtaaagacgg ccgaaaaggt caacgctgac cgcatcgact cggcgaacaa gacggcgtca
     1381 ttggccgact cggcttcggt gccgaccaag caggatgcac aattcatggt aaaagccaaa
     1441 agcgggaacc agttggaggt gacgctgggg gagctggcgc agaccaatgc tcagagcctg
     1501 gcggtcaaac ggttcgggct catgatggtc cgggaccata ccgaagggga aaaggagctg
     1561 aggtcgctgg ctatcgccaa gagggttgtc atgccggata ccctctccaa acaacagcaa
     1621 aaagaggcag acgatctgaa aaagaagacc gggcacgatt tcgacaaggc ctatatcgac
     1681 ctgatggtcg acgatcataa ggaagacatc gacgaattca cgaaggccgc gcagcaagcc
     1741 aacgatccgg atatcaaggc gctagccgca aagatggtgc cggtgctgaa agaacattat
     1801 gactcggcgc ggatcctcaa acgcaacggg gggagatgag accaggaggc gggggccgca
     1861 aaagaaaaaa aataaggccg cgcgggcagc gcggcctgtc ggctaattca ggataattcc
     1921 tccagcaact cctcgtttaa ttcgatcttc ccggcatcca tattctcctc cagatggcgg
     1981 acgctggacg taccgggaat aagcaggtta ttaggggaat ggtagaggag ccaggccagc
     2041 gccagctgat gtggggtgac ccccagccgg cccgccacag actcgatctt cgtcatattc
     2101 tccagtttac ccgcattcag cgggttccag ggaatgaagg ccttgccggt ctgttcgcaa
     2161 tattccaaca ccggctccca cttgcggaag tcctgactgt acttattctg gacggagaca
     2221 acttccacat attcttcagc ctttttgata tcctccacgc cgacctcgga cagaccgaca
     2281 tgtttgatca acccttcttc ctgaacacct tgcagaaagg aaagggtctc ctcgaacggc
     2341 acggtcggat cgatacggtg cagctgatag acgtcgatgc ggtccagccg cagccgggcc
     2401 aggctgcctt ccagcgcctc tttcaggtga tcgggactgg agttgatatg ccattgattc
     2461 ggaccacttc ggagaaagcc gcctttggtg gcgatgacca atcctttggg ataggggtag
     2521 agcgcttcgg cgatcagctc ttccgataca tcgggcccat agctgtcggc cgtatcgatg
     2581 aagtttacgc ccagctcgac ggcccggcgg agtacgcgga tggcctcgtc atggtccttc
     2641 ggcggacccc agatgccctg acctgtgatg cgcatggcac cgaaccccat ccggcggacg
     2701 gtcagatcgt cgccaagggt aaattcttta cggaaactta cattagtact catataattg
     2761 aatattttaa gcggaaagag gcgggacgga agagatcaaa ggacggcggg gtagaaaaca
     2821 acagtttgta cagcgaaatt gttcatcagg tgtccccggt ttattttata ttcgttatgc
     2881 tccaaatatc gcttcatgag aacaaccttc ctctgctgtc ttctgctcgc gaccttcatc
     2941 gccttcagcc agaaaaagcc gctggacccg tccgtgtacg atggatggca gagtattgga
     3001 gagaagctac tctcccccga cggtaaatgg ttggtctata cgatcactcc tggggaaggg
     3061 gacgccagtc ttgtcatacg ggcgaccgat ggcgcttatg caaaagaaat accgcgcggc
     3121 gccggggccg catttacgga tgacaaccgg ttcgtcgtct ttcatatcaa acctatgtac
     3181 cgtaatattc gcgaggcccg tatacggaaa aagacgccgg accagtcacc caaaggcagc
     3241 ctggcatgga tcgaattggg gaccggtcgc ctcaccattt tccccagggt gaagtcctac
     3301 aagctgcccg aaaaggacgg cgaatggatc gcataccttc gcgaaaaacg gttacccggt
     3361 accggcagac cggacccggg aaggagaatg gcgaacggcg actcggggcg gagaatgacg
     3421 aacggcatac cggtcgttaa aagcacacct ataaacactg catatgttca ggaattactg
     3481 gaaaatcgcc ataagaagcc tcctgaaacg gaaaggcttc accctgatca acatcctcgg
     3541 gctggccacg ggtatggccg tttgcctgtt gatcctgctg ttcatccgtc gcgagacggg
     3601 ttacgacaat ttcgagccgg cgggagaccg gatctacagg gtcgtgctcg aaaggaaata
     3661 tcccggccgg agcacttcct atgcgatcat accccaatcg ataggcggcg ctattcaaaa
     3721 agagtttccg gaggtgcagg cttccaccgg tgtccaggat atcaccggca acgccaattt
     3781 cctggtcagg atcggggatc gcaattttga agagacgcgg gtgctgctgg cggactctct
     3841 ctttttccgg gtgttcccga tgcatctgct ggcaggcgat acggctaccg ccctgcagca
     3901 accgaatatg gcgatcctga acgagaccac cgcgacgaaa ctattcggat cggccggagc
     3961 cgcgttgggc aaaaccttcg aatcggagaa caaacaacac taccaggtct ccggtgtctg
     4021 cgcggactgg ccggataatt ctcacatcag cttcaacatc cttttgtcgg ccgccagttt
     4081 cccttttacc aaagtcccga attttaccgg attttccacc tacacttacc tgttgctgcg
     4141 tccgaatacc gatcccgcgg cgctggaagc caaattcccg cagatcgtcc aaaaatacgt
     4201 ggccggtgaa ataggccgga gtttcggaat gagcttcgcc cagttcgagg catccggtaa
     4261 cggatatcac tattatatgc aaccgctggg acagatccac ctcatttccg atctggaagc
     4321 ggagatgcgc cccaacggca gcaaacgggc cattacaatc ttcggtatca tcgccatatt
     4381 tatcctcggc atcgcctgcg tcaacttcat caatctctct acggcccggt cgatcgagcg
     4441 ggccagggaa gtaggcatcc gcaagacctt cggttccgaa cgacggtcgc tcatcgggca
     4501 attcctgctg gagtcgacgg tggtcagcat cctcagcgtt gcactcgcgg cggggatcct
     4561 cacgctggcg ctgccctggt tcaaccgcct ggccggtcag cagctgtcga tcctcgcctt
     4621 ccttactccc gtgaacatat cgggtatcct tgctttcgga atagcggtag gagtgctcgc
     4681 gggcatctat cccgccttcg tgctctcgtc cttccggccg ataacggtgc tgaaaggcag
     4741 atttcaatcc gcacggtatg ggatcgtcct gcgcaatgcc ctcgtcgttt ttcaattcgc
     4801 catatccatc atcctcatca tctgcaccgt cgtcgttcac caacagatgc gtttcatgct
     4861 gggggataag ctgggtttta ccagggacgc gatcgtagag atccaacggt ccgatctcct
     4921 ggataagaat accggggctt tccggaatgc gttgagaagc attccgggag tggaagagat
     4981 cggagggtca tcgagcatgc cgggtgatgc gaattttttc ggcattgcct ggcaacaggt
     5041 gggttccaca gaatcgatga ctggcagagg gatcgtcgtc gacgaccaat tcgccaatac
     5101 cctgggattg acgttaaaag aagggaggtt tttttctaag agctacgcga ccgactcgct
     5161 ttcggtagtg ctgaatgaaa aggccgtgag cgcactgggc ttaaaagaac ccgtcatcgg
     5221 cacccggctg acgaccacat cgggattcct caatccacgg cccgattctt tctataccta
     5281 tacggtggta ggcgtggtga atgattttca tttccagacg ctgcatcagg cgatagcccc
     5341 gctggtcatg accgatgcgg tgaagttcgg ggacgcgacg gcgataacgg ccgtcaggct
     5401 aaagggcagt gagttcagct ccgcgatagc gtcgatcgga cagcaatgga aacgttttgt
     5461 tccgcaacgg cctttccgct atacctttct cgaccaacaa ctggcggcgc aatacaaggc
     5521 cgagcaaacg ttgcaacggc tgttcaccgt attttccctg ttggccatat ttatagcctg
     5581 tatcggactt ttagggcttg ccgcctattc gacacagcaa aggatcaggg agatcagcat
     5641 ccggaaggtg ctcggggcgc gtccgggatc gatcatcggt ctgttgtcgg tgggtttcat
     5701 ccgcctggtg acggtatcgg cattcgtcgc cttcccgctg gcctggctcg ccatgcattc
     5761 ctggttacag ggctttgcct atcgggtcgg gctggggtgg tgggtctttg ttcttgcctg
     5821 gctcctttca cttgtcatta cacttgtcac catcagcctc caggccatcc gcgcggctgg
     5881 cgtcaacccg gtaaaaattt tgcgatccga gtaagacaag ccatatgatc aaaaactatc
     5941 tcctgacggc ctggcgcaac ctggtcaaac ataagctgaa tgcgtccatc ttagtgatcg
     6001 gactcacggt ggcgttcacc tgttgcaccc tgctgttcct gatggtacgt tatgaattct
     6061 cctatgaccg ctggcatacg aagaaggaca ggttatatga agcctacgaa cgaaacgaga
     6121 gccccgacgg agaacgacgc agggaaggct ttccctaccc ggcggcccgg acgatgaaag
     6181 cggagatacc ctccatcgta cgcagcacct cgttcatgtc tgcaggcggc gccatacgct
     6241 acaggggtaa ggaggtttat cagcggctgg tactggtgga cagcgatttt ttcgccatgt
     6301 tctcttttcc cgtcgtggcc ggcaatggga tctctccgct ggcaaatacg gacaaagtgg
     6361 tcatcaatca aacggtcgct acggcactat tcggtaagga tgaggccgtg ggccgacatg
     6421 tacaggtgaa gatcagcggg caatggaaag cgcttacggt tgccgccgtc ttacgcgatg
     6481 ttccggccaa ctcgtcgttg caattcggca tattggcccg tatcgagata cacaaggatt
     6541 atccgaccca gaaagacgac tggggcgaca gtcatcatcg cgtctatgta gagctggctc
     6601 ccggagcgac ccaacaagtg gctgaagccg gtctgcggga gatggtccac cggcataatc
     6661 tttccgacag cgactttctg aagagcgaag gttatctccg tgaccggaac ggcgattatc
     6721 tttctatccg gctcgtgcgg ctgcccgacg tgcatttcga tggcgtgctc ggaggacgtt
     6781 cgtccatcga caagtcctat ctctatacga tgatgctcat cgccctcatc gtgatggtga
     6841 tcgcctgttt caatttcgtc aatctcaatg tagcacggtc ttttacacgt gccagagagg
     6901 tgggtgtccg aaagaccatc ggcgccggcc ggcgcgatat ctatttgcag ctgtggctgg
     6961 agagcctgat cctgtttgtc atcgccctgg ttttatctat gatcgcatcg ggatccctgc
     7021 tccatccctt caacgacctt tttaccgaaa agctgcggct gtcctcgctg ctggaccctg
     7081 cggtgctcgg gatcatcgcg ggtgcgactg tcctgatctc ctttttggcc ggggggtacc
     7141 cgggctggat ggtatcccgg tttcatgtcg tggatgtatt gaaagggaag gtctccatgc
     7201 gccgcaacag tttcctgcgg agcggtctga tcacgctcca gttcatcatg gcggcggctt
     7261 t
//
LOCUS       DASYSV010000081        15811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1298684, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000081 DASYSV010000000
VERSION     DASYSV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 15811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15811
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298684"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VGQ51_02465"
     CDS             complement(<1..203)
                     /locus_tag="VGQ51_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9035449.1"
                     /translation="MKKNVVVIGGGIIGLGSAYYLQKAGHQVTVLDRSDFMDNCSYGN
                     CGYVCPSHFIPLATPGIVRQGLK"
     gene            381..1271
                     /locus_tag="VGQ51_02470"
     CDS             381..1271
                     /locus_tag="VGQ51_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9035450.1"
                     /translation="MKVLQFTIPVPHDRTVIVQKDELPHFYPHLHRHQEVQLTWVQQG
                     EGTLVAENNMYAFRPGDIFWLGVNLPHVFKSDPSYFSPSSRKKVRTLNIFFNIKGQLD
                     SFFELPEIKNLKNFLANRQTGFKVPPQHVTEVSAKMLSMQNSAGVDQLIHFIELLKQL
                     SSFPELEPLSSCSQPSGYSEHEGIRIGTIYNYIIQMYDKPITLEDVAKQAHMTPQAFC
                     RYFKKHTLHTFVSFLNEVRINEACKKLTDGNYDSIATVAYNCGFNSITNFNRVFKSVT
                     RKSPSEYVDSYFNLNAAPVE"
     gene            complement(1314..3671)
                     /locus_tag="VGQ51_02475"
     CDS             complement(1314..3671)
                     /locus_tag="VGQ51_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEV9035451.1"
                     /translation="MKRGLLLSVIVLAGCGLRAQSYVPEYGNASVKVKPVVAIRAMPF
                     SIKDVRLLDGPFRQAMEADARYLSIIEPDRLLSEFRAHAGLTPRAPKYGGWESSGLAG
                     HTLGHYLSACSMQYASTGDTVFLHRVNYIVSELETCAKTRGGYVGAIPREDTLWAEVK
                     AGHIRSHGFDLNGAWSPWYTVHKVMAGLLDAWLYCGNKEALNVERGMADWTGRIVGGL
                     PDSTIQKMELCEYGGMNDVLANTYAITGDKKYLDLSYKFHDRRILDSLSHGLDDLAGK
                     HSNTQIPKVIGCARQYELTANAGDSAIAKSFWSIVVGHHTYATGGNSDYEYLGAADKL
                     NDELTDNTEESCNTYNMLKLTRHLFAWRPTAALMDYYERALYNHILATQDHATGMTTY
                     FTPLRMGGHKEYSDSFNTFTCCVGSGMENHVKYGESIYSRGSDGSLYVNLFIPSRLSW
                     KEKGVTVEQRTLLPREDKTELNITASHPAMFTLRIRRPHWSGPVSLSVNGKPVTVVVG
                     EDGYLWVRRTWKNGDKVEVRFPESFYTMAIPDNKDRIALFYGPVLLAGELGDKEPDPV
                     SGIPVLVSSSEDPNQWVHRLADKDRLVFRTSAVGHPGDITLIPFNRTENEYYSVYWDY
                     FSPAGWTAQQEKYAAERKSEQDLEDRTVDRLRLGEMQPERDHSFTGEGLQTGDGHGRK
                     WRSAEDGGWFSFVIKVDSAAQNTLLCSYWGDDHRGRIWDIQVDGQTIATQNLTSFKQS
                     KFYEIAYPVPTELVHGKQTVTVRFAAHGKRTAVGPVSGVIRMVRN"
     gene            complement(3673..5862)
                     /locus_tag="VGQ51_02480"
     CDS             complement(3673..5862)
                     /locus_tag="VGQ51_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV9035452.1"
                     /translation="MRSIARFVFIVAFFFFLTGTVSAQRGGFGVKWGKGGDKYYALES
                     NSIVCYSLPGFQRTVAVEASQMVPSGRSEPLPIQGYSFSDDDKKLLIYTNSKKVWRQN
                     TRGDYWVLDLATHTLKQLGIGRPASSLMFAKLSPDGTKAAYVSEHNLYVEDLAGGTIT
                     PLTTDGAFRMINGTFDWAYEEEFSCRDGFRWSPDGKSIAFWQLDATKVKNFLMIDNTD
                     SLYPFTVPVEYPVAGEDPSPCRVGVVDVASKKITWMDVPGDNRQHYIPRMEWADNSKE
                     VVLEQLNRKQNDARVFLCDASSGAARQIYEESDSTWIDVKHRYSDDPTGWEWIDGGKD
                     FLWVSEKDGWRHLFKISRDGKKETLLTKGNYDLISLKAIDEKGGFAYFMASPDNATQA
                     YLYRVPLDGKGKLDLVSPAMQKGTHNYNISPNAAYATHNFSNHLYESVSEWVSLPKHT
                     TIKTNEQPARRGNGRPQYPVKMFQLTTDDGVTMDGWMILPKDFDSTKKYPVLFNVYTE
                     PAATTVKDVAGSAGTPLYAGDIAADGYIQISLDGRGTPAPKGAAWRHCIYRKIGIINI
                     HDQAMAARKILQWPFVDSSRIAVHGWSGGGSATLNLMFQYPDIYKTGISIAPVADELL
                     YDNIYQERYMGLPQENREDFVRGSPLYYAKNLRGNLLVIHGTGDDNVHYKGTEELINE
                     LVKDDRQFQVMVYPNRTHAIREGEGTSLHLSHLFTAYLRAHCPGGGR"
     gene            complement(5859..7604)
                     /locus_tag="VGQ51_02485"
     CDS             complement(5859..7604)
                     /locus_tag="VGQ51_02485"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV9035453.1"
                     /translation="MNVKGLLPALCLTAPLFCLAQAGQGGSQNPDSVRARRQRMIERA
                     RYEYEHKVFTHADTLRGSNGAERSWWDIQRYDITVKPDYPTKTTTGDDLITYKVVSAN
                     QPAMQIDLQAPLHIDSVFGDGHRTLSFTQEGNAWHVHSPRQKVGATATVRVYFSGTPT
                     EGKRPPWNGGWTWTKDSLGRPWMTVTCQGLGASVWYPCKDYQGDEPDKGASMTMIVPD
                     TLVGVSNGRLQFKKDNGDGTTTYKWAVVNPISNYCLIPYVGKYVTFHEDFQGEKGHLD
                     LDYWVLDYNLTKAKAYLPEQVHNMLHAHEHWMGPYPFYEDSYKLVDVEHTGMEHQSAV
                     AYGNHYAFGYRGRDGSGTGIGMRYDFIIIHESGHEWFGNNLTTKDLADMWVHEGFTNY
                     SETLFAEYMWGKEAGNDYCVGTRRGIRNDRPIIARYGVNEEGSGDMYPKGGNMLQSIR
                     HSIDNDEKWRQILRGLNKDFYHQTVTSAQVEHYVSQHAGYDYGKVFDQYLRTIQIPDL
                     EYYFSGDHKKVFYHWTNCVAGFDLPLVLAGDGVKIRIVPTEQWQSRDLKGDEAALFGE
                     EAIKKMYYIGVKQKS"
     gene            complement(7618..9057)
                     /locus_tag="VGQ51_02490"
     CDS             complement(7618..9057)
                     /locus_tag="VGQ51_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEV9035454.1"
                     /translation="MRKLFIISLFAGGLASSCTKNTQNLNIDTKHAVSVPATSLFLAG
                     EKNLSDDVTTTNIGVEPFRVFSQTWTETQYTTEAKYVLSAYNSPDNWWGNLYSGVLNN
                     FANAKAAFPVSVTDPGTLRNDLIITDILEVYTYNLLVSTYGNIPYSQAESRSIPFPKY
                     DDAKTVYSDLLTRLDTCIAGLNSSSPAMGSADQIYKGNIAKWKKFAATLKLKMAMVLA
                     DTDPTTAGKKVQEAVATGVFAANSDNATVAYQSSPTGNTNPVWQAIVNSGRHDFCPAN
                     LLINTMNSWNDPRLPLYFTTVGGVYQGGIPGATNQFSVFSTFSAQWIAPTYPGDVLDY
                     AETNFLLAEAVERGFLTGGNTAEQYYNAGVTASITFWGGSASDASTYLAQSAVAYTTA
                     AGDYKQKIGYQEWIAMANRGWDSWTSIRRLHQPNIDVVNPPIGATGPMPIRFYYPLRE
                     QTANPVNWAAAAKTISGGDVVTSKLFWMP"
     gene            complement(9085..12423)
                     /locus_tag="VGQ51_02495"
     CDS             complement(9085..12423)
                     /locus_tag="VGQ51_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9035455.1"
                     /translation="MRKRLFSALCLIMASLLFLNANAQKRTITGKVVDSVGVPIPNAS
                     VRLRSTHAGVNTVEDGTFRITAASTDILLVTVVGFEPQEIKVGSQDQISITLHRTTQN
                     LNEVVVTALGIRRTRNSVPYAMQQISGDQVNKVVTTNFVDNLAGKVAGLTVTSSNTMG
                     GSNNVILRGMKSLTQTNQALFVVDGVPYNNGNNSAGGYDVGNAVSDINPNDIESVSVL
                     KGAAASALYGSLASNGVILITTKRGSRLNKGLGIVVDEGVTVGTPDKSTLPSYQLQYG
                     EGSGSAGAGIAGNPNAFFYWYPTSFSNGQAVQIVQTDVDQMTGPAYDPSMMVYQWDAF
                     SPGNPNWGKATPWKPAAHFRPTDYFVTPITNSTSVFLDGGNDRGTFKMGYTRSTDNGY
                     LPNSSLLKNQLVFGATYNLARNLTATGDFSFTEDDAIGRYGYGYAPGNGTQYNPMTDF
                     RQWWQTGIDLHEMKADYFRTNTNATWNWQVPAYTANAPGIIGKPAFHDNPYWVRYTNF
                     ESDGRQRYFGYASLTWRVAPFLNIMGRVSRDNWNQLVETRYNVGTYGTPSYGRTNSTF
                     TETNYDFLANFDKNLNESLNLKALLGANIRQDLSTSIAASTNGGLVVPGFYALGNSVN
                     PINAPTESYVPKEVDGLFAGATLTWKDMVTLDATIRGDKSSTLPTANNRYYYPSVSAN
                     WVFSKLMPETEWLTYGKLRVNYASVGGDAPPFSLLNTYVAGNPFNSQTIFTSPTTNNN
                     PSLVPERNNTYEVGAEMSFLQGRVGFDATYYHAQQVNQIMPVSVSTSSGYSSFYVNGG
                     TVQNQGVELTVNLVPVKTQNFEWDMTINWSTNKSKVLSLYGGQPSFLITGYQNSVQLV
                     AEVGKSYGILRGTDYKYLNGQGQVDTLGQGKRLIDANGYPVLSSNRLSDIGNINPDWM
                     GGIYNSLRYKNLSFGFLIDVKQGGSVYSLDQDYGANAGLTTHTGGYNKNGVGVRAPLS
                     QGGGYLFEGVTADGKANATLVDASDVNGKAVAGHVAYPWGSPAYEAARTFIYDASYIK
                     LRELNLTWSVPQNILSGANFIKGLDLSLTGRNLWIIHKNLPDADPEQGVPISGSYGAN
                     GSQGFQSGAYPTFRTYGFKVKVKF"
     gene            complement(12606..12845)
                     /locus_tag="VGQ51_02500"
     CDS             complement(12606..12845)
                     /locus_tag="VGQ51_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035456.1"
                     /translation="MTTLVGIFFSLISLLGLFKGSSKRVLRRIVITAFCIFLVLAQVL
                     FIYSRVRMESQQFIGFSGLLSGIILILSFQKEEAW"
     gene            complement(12968..13189)
                     /locus_tag="VGQ51_02505"
     CDS             complement(12968..13189)
                     /locus_tag="VGQ51_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV9035457.1"
                     /translation="MDKQEGALRSFGRQLSALREEQGMSITELASRSGLQPADITSIE
                     SGEKDIHITEIFRLAAALGVPPSQLLGSA"
     gene            complement(13357..13593)
                     /locus_tag="VGQ51_02510"
     CDS             complement(13357..13593)
                     /locus_tag="VGQ51_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV9035458.1"
                     /translation="MITPADKKILKRFGENLRKLRKDKGLSLREMSYACSIDNSKIAK
                     IEKGMINITFTTLLQLAVALEAHPAVLLEYAPAE"
     gene            complement(13691..14818)
                     /locus_tag="VGQ51_02515"
     CDS             complement(13691..14818)
                     /locus_tag="VGQ51_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEV9035459.1"
                     /translation="MKIVFPALIAGFLVTAVFSSGDPAWALTGFIKSDSINPVLTPGT
                     GIFTDPVLHRTIAWEAKDVFNPATVVRNGKLYLLYRAQDAVGHPAGTSRIGLAESIDG
                     YHFTRRAMPVLYPDNDAFRKYEWEGGCEDPRVVEDGKGVYYMTYTGFDGHTARLLVAT
                     STDLVHWTKHGPAFGKAMHGKYLDKWSKSGSIVSRYEGARVVAARIKGKYWMYWGDQF
                     IWAAVSDNLIDWTPVEKAPGQTDPVELRGQAREMPALKIVLPTRKGKFDSDLVESGPP
                     AMLTDQGILLLYNSRNIPSIGDPALPEGTYTASEALFDSADPTHLLHRLDHYFLRPDK
                     PYELTGQVSSVCFIEGLARFDGRWMLYYGTADSKIAVATRP"
     gene            complement(14843..15076)
                     /locus_tag="VGQ51_02520"
     CDS             complement(14843..15076)
                     /locus_tag="VGQ51_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035460.1"
                     /translation="MFNKFQIHFNYDGRQYKGVIWPLQAGIQHRIPTTFQVILNNVYC
                     GLIKRRGADWETDSPKCAIMVDIIGNQIYDWYE"
     gene            complement(15139..>15811)
                     /locus_tag="VGQ51_02525"
     CDS             complement(15139..>15811)
                     /locus_tag="VGQ51_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEV9035461.1"
                     /translation="KNVFLRHPFVYLVEAADDICYRIIDFEDAHRLNIISIDTIRDLF
                     LSFFDGETGYDARDRVEEVFRGIRDDNQKVQFLRAKLINLLISRVRDIFMEKEADLLE
                     GTVSRPLIDFLPAREAALIRTIDAYSVRHIYNHRSVVEIEIAGYHVIGGLLREFMGAV
                     LNPRSAKSIKLLQLIPEQFIVSGSSESLYTDLLSVVDFIAGMTDLYAVDIYRKITGIE
                     FPRVG"
BASE COUNT         3544 a         4358 c         4169 g         3740 t
ORIGIN      
        1 cacttcaatc cctgacgaac gatgcccggg gtagccagcg ggataaagtg gctggggcaa
       61 acatacccgc aattgccata ggagcaattg tccataaagt ccgatctgtc caaaacggta
      121 acctgatgtc cggccttttg aagatagtaa gcagagccga gtccgatgat ccccccgccg
      181 ataacgacca catttttttt cataattccg caaatttagg gctaatcgaa cccccgattt
      241 tatcccttcc agcgatattt taaccagttt ttacccgatt ttcacagcat aatgataata
      301 tccgtaaatt gagtaatttc atacagaggc taatggtaac gaaatctttg tcacacaacc
      361 accaccaaaa ctgatcagcg atgaaagtac tgcaattcac catcccggtg ccgcatgaca
      421 ggacagtcat cgttcaaaaa gatgaactgc cacattttta tccgcatctc caccggcacc
      481 aggaagttca actgacctgg gttcagcagg gagaagggac cctggtcgcg gaaaacaata
      541 tgtatgcctt ccgtccggga gacatcttct ggctgggcgt caaccttcca cacgtattca
      601 agagcgaccc ctcctatttt tcgccgtcca gccgtaaaaa ggtccggacg ctgaatatct
      661 ttttcaatat aaagggtcag ctggactcct ttttcgagct gccggagatc aagaacctca
      721 aaaatttcct ggccaaccgg cagaccggtt ttaaggtccc cccgcagcat gtgaccgagg
      781 tatcggcgaa aatgctcagc atgcagaaca gtgccggtgt cgatcagctc atccatttta
      841 tcgagctgct gaaacaattg agctctttcc ccgaactgga gccgctgtcc tcctgttctc
      901 aaccttccgg ctacagcgag catgagggca tccgtatcgg gaccatttac aactacatca
      961 tccagatgta tgacaaaccc attacgctcg aggacgtggc caaacaggcg cacatgacgc
     1021 cacaggcttt ctgccgttat ttcaaaaaac acactttgca caccttcgtc tccttcctca
     1081 acgaggtgag gatcaacgaa gcctgtaaga agctgaccga cggcaactac gacagcatcg
     1141 ccaccgtcgc ctacaactgc gggttcaaca gcatcaccaa tttcaaccgc gtgttcaaaa
     1201 gcgtcacgcg aaaatcaccc agcgagtacg tagacagcta tttcaatctc aacgccgcac
     1261 ccgtcgagtg agggcgcgcc cgtcggggga aggagcacgc gccgggtgag ggcttagttc
     1321 ctcaccatac gaatgacgcc ggacacaggt ccgacggccg tccgtttccc atgcgccgca
     1381 aaccgcaccg tcacggtttg tttcccatgc accaattcag tgggtacggg atacgctatt
     1441 tcatagaact tactttgttt gaaggacgtc aggttctgag tggcgatggt ctgcccgtca
     1501 acctggatat cccagatgcg gccgcgatga tcgtcccccc aatagctgca tagcaaagtg
     1561 ttttgggcgg cgctatccac tttgatcaca aaggaaaacc agccgccatc ctcggcggaa
     1621 cgccatttcc gtccatgtcc gtcacccgtc tgcaacccct cgcccgtaaa tgaatgatcc
     1681 cgctccggct gcatttcgcc aagacgaagc cggtcgacgg tcctgtcttc gaggtcctgc
     1741 tcgctttttc tttctgccgc atacttctcc tgctgagcgg tccagccggc gggtgagaaa
     1801 taatcccaat acacggaata atattcattc tccgtcctgt tgaatggtat caacgtgata
     1861 tcccccggat gcccgacggc ggatgtgcgg aagacgagcc ggtccttgtc cgccagacga
     1921 tgcacccatt gattcgggtc ttcgctggaa gataccagca cgggaattcc gctgaccgga
     1981 tcaggctcct tatcccccaa ctcgccggcc agcaggacgg ggccatagaa taaggcgata
     2041 cggtccttgt tatcggggat cgccatagta taaaaggatt cgggaaaccg gacctcgacc
     2101 ttatccccat tcttccacgt acggcgcacc cacaaatagc catcttcgcc gacaaccacc
     2161 gtgacaggtt tcccgttgac cgacaggctc accggtcccg accaatgcgg ccgccggatg
     2221 cgcagtgtaa acatagccgg gtgtgatgcg gtaatgttga gttcagtctt gtcttcgcgg
     2281 ggaagcaggg tacgctgctc caccgtgaca cccttctcct tccagctaag ccgggagggt
     2341 atgaaaagat ttacatacag actgccgtcg ctgccgcggg agtaaatact ttctccatat
     2401 ttcacgtggt tttccatccc cgagcccacg caacaggtga acgtattgaa agagtcacta
     2461 tattccttat gcccacccat gcgaagcggt gtaaaatagg tcgtcatacc cgtcgcatga
     2521 tcctgcgtgg caaggatatg attatacagc gcgcgctcat aatagtccat cagggcggcg
     2581 gtcggacgcc aggcgaaaag atgcctggtc agcttcagca tgttataggt attacaggac
     2641 tcctccgtat tatcggtgag ttcatcattc agcttgtccg cggcacccag atattcataa
     2701 tcgctgttgc cgccggtggc ataggtatga tgcccgacga cgatggacca gaaggacttt
     2761 gcgatggcgc tgtcgccggc gttggccgtc agctcatatt gccgggcgca accgatcacc
     2821 ttcggtatct gtgtattgga atgttttcct gcgagatcgt ccaaaccatg cgagagcgag
     2881 tccagtatcc gccggtcgtg aaatttatac gaaagatcga gatatttttt gtcgccggtg
     2941 atggcatacg tattggccag cacatcattc atgccgccat attcgcaaag ctccatcttc
     3001 tggatcgtac tgtcggggag accaccgacg atacggcccg tccagtcggc catgccgcgt
     3061 tccacgttca gggcttcttt attgccgcaa tacagccagg cgtccagcag acccgccatc
     3121 actttgtgca ccgtatacca gggcgaccat gcgccgttca gatcgaaacc atgcgaccgg
     3181 atatgccctg ccttcacttc cgcccacagc gtatcttccc gggggatggc tcctacataa
     3241 ccaccacgcg tcttcgcgca agtctccagc tcgctaacga tatagttcac ccgatgaaga
     3301 aagacggtgt cgcccgtaga tgcatattgc atcgaacagg ccgagagata atgccccagc
     3361 gtatggcccg ccagacccga ggattcccat cctccatatt tgggcgcgcg tggggtcagg
     3421 ccggcatgcg ccctgaactc cgacaacaac cgatcgggct cgatgatcga caggtatcgg
     3481 gcatcggctt ccatcgcctg tctgaaaggc ccgtccagca gacgcacatc cttaatggaa
     3541 aagggcatgg cgcggatcgc gacgacaggc tttaccttta ccgatgcatt cccatactcc
     3601 ggcacataag actgcgcccg cagcccgcag cccgccagaa cgatcaccga taacaataaa
     3661 cctctcttca tattatcttc ctccgcccgg acaatgcgcc cgaagatatg ccgtaaacaa
     3721 gtgtgataaa tgcagcgaag tcccttcccc ttcgcggatc gcatgcgtcc ggttgggata
     3781 gaccatgacc tgaaattgcc ggtcatcttt tacgagctca ttgatcagct cctcggtacc
     3841 cttataatgc acgttgtcat cgccggtgcc atggataacg agcaggttac cgcgcaggtt
     3901 cttcgcataa tacagcggcg aaccgcgtac gaaatcctcc ctgttctcct gcggcagacc
     3961 catatagcgc tcctggtaga tattgtcata caacagctcg tccgccaccg gcgcgatgga
     4021 aatgcccgtc ttatagatat cgggatattg aaacatcaaa ttcagcgtag ccgagccgcc
     4081 gccgctccac ccgtgtaccg ctatacggct gctgtctaca aacggccatt gaaggatctt
     4141 tctcgccgcc atggcctggt cgtggatatt gatgatgccg atcttccggt agatgcaatg
     4201 ccgccaggcg gcacccttgg gagccggcgt gccgcgtccg tcgagactga tctggatata
     4261 tccgtcggcg gcgatatctc cggcatatag cggcgtgccg gccgatccgg ccacatcctt
     4321 cacggtcgtt gcggcaggtt cggtatagac gttgaaaaga acgggatatt tttttgtgct
     4381 gtcgaaatcc ttcggcagga tcatccagcc atccatggtg acgccgtcat cggtggtcaa
     4441 ttggaacatt ttgaccgggt attgcggacg cccgttaccg cggcgggccg gctgctcatt
     4501 ggtcttgatg gtcgtgtgtt tgggaaggct cacccactcc gataccgatt catacaggtg
     4561 attggagaaa ttgtgtgtcg cataagccgc attcggagat atattataat tatgcgtccc
     4621 cttttgcatt gccggcgaca ccaggtcgag tttacccttg ccatccagcg gtacacgata
     4681 caaataggcc tgcgtcgcat tgtcgggcga ggccatgaaa taagcaaatc cgcctttttc
     4741 gtcgatggct ttgagactga tcaggtcata gttgcccttg gtgagcaagg tctctttttt
     4801 gccgtcacgg cttatcttga aaagatgacg ccagccgtcc ttttcggaga cccagagaaa
     4861 atcctttccg ccgtcgatcc attcccagcc ggtggggtcg tcgctgtaac ggtgtttcac
     4921 gtcgatccag gtgctgtcgc tctcctcgta aatctgtctg gccgctcctg aggatgcatc
     4981 acagagaaat accctggcat cgttctgctt tctgttcagc tgttcgagta cgacctcctt
     5041 gctattgtcg gcccattcca tgcgcggaat atagtgttga cggttgtcgc cgggcacatc
     5101 catccaggtg atctttttcg acgccacatc gacgaccccg accctgcagg gcgacgggtc
     5161 ctcacccgct accgggtatt cgacaggcac cgtaaaagga tacaacgaat ccgtattatc
     5221 gatcatgagg aaattcttca ccttcgtcgc atccagctgc cagaaggcaa tgctcttgcc
     5281 atccgggctc caccggaaac catcccggca gctgaactct tcctcatatg cccagtcgaa
     5341 ggtgccgttg atcatacgga aagcgccgtc ggtcgtcagc ggagtgatgg tccctccggc
     5401 cagatcttcg acataaagat tgtgctcgct gacgtaggcc gccttcgttc cgtcgggtga
     5461 cagcttggcg aacatcaatg aagaggcggg ccttccgatc cccagctgtt tgagcgtatg
     5521 cgtcgccaga tcgaggaccc aatagtcgcc gcgggtattc tgacgccaca cctttttact
     5581 gttggtatag atcagcaact tcttatcgtc atcgctgaat gaatagccct gaatggggag
     5641 cggctcgctc cttccggaag gcaccatctg agacgcctcg acggcaaccg tacgctgaaa
     5701 accgggcaga ctatagcaga cgatggaatt gctttccagg gcataatact tatctccgcc
     5761 cttcccccat tttacgccga aacctccacg ttgcgcgctg acggttcctg tcagaaaaaa
     5821 gaagaacgcg acgataaaaa caaaacgggc aatcgatctc atgatttttg tttgacacca
     5881 atgtagtaca tttttttaat agcttcttct ccgaacagcg ccgcttcgtc tcctttcagg
     5941 tctctgctct gccattgctc cgtcggcacg atccggatct taacgccgtc acctgccagc
     6001 accagcggca gatcaaaccc cgctacacaa ttcgtccagt gatagaacac tttcttatga
     6061 tccccgctga aataatattc gagatcgggt atctgtatcg ttcgcagata ctgatcgaac
     6121 acttttccgt aatcgtaacc ggcatgctgc gagacatagt gctccacctg cgcggacgtc
     6181 accgtctgat gataaaagtc cttgttcagc ccacgcagta tctggcgcca tttttcgtcg
     6241 ttatcgatgc tatgccggat gctctggagc atattgcccc ctttggggta catgtcaccg
     6301 ctgccttcct cgttgacacc atagcgggcg atgatggggc ggtcgttacg gataccacga
     6361 cgggtaccga cacaataatc atttccggct tctttgcccc acatatactc tgcgaataat
     6421 gtttcgctgt aattggtaaa gccttcatgc acccacatgt ccgcgaggtc ttttgtggtg
     6481 aggttattgc cgaaccactc gtgaccggat tcatgaataa tgatgaaatc gtaccgcatg
     6541 ccgatgccgg taccgctgcc atcgcggccc cggtacccaa acgcataatg attgccatag
     6601 gcgacagcgc tctggtgttc catacccgtg tgttcgacat ccaccagctt atagctgtct
     6661 tcataaaagg gatagggtcc catccaatgc tcatgcgcat gcagcatgtt gtgcacctgt
     6721 tcgggcagat aagctttcgc ttttgtcaag ttataatcca gcacccagta atcgagatcg
     6781 agatgtccct tctcaccctg gaaatcctca tgaaaggtga catatttccc gacatagggg
     6841 atcagacaat aattgctgat ggggttgacg accgcccatt tataggtggt tgtcccgtct
     6901 ccgttatctt ttttgaattg cagccggccg ttgctgacgc cgacgagggt gtctggaacg
     6961 atcatggtca tcgacgcgcc cttatcgggt tcatcgccct ggtaatcttt acagggatac
     7021 cagacggatg cgccgaggcc ctgacaggta acggtcatcc acggacgccc gagcgagtcc
     7081 tttgtccatg tccatccgcc attccatggt ggtcttttcc cctctgtcgg agtaccggaa
     7141 aaatataccc gcacggtagc cgtggctccg accttttgcc gcggtgaatg cacgtgccag
     7201 gcgttgcctt cctgtgtaaa agagagcgtt ctgtgcccat cgccaaagac actgtcgata
     7261 tgaagcgggg cctgcagatc gatctgcatg gccggctggt tcgcgcttac gaccttatac
     7321 gtgatcaggt cgtcgcctgt ggtggtcttt gttggataat ccggtttcac cgtgatatcg
     7381 taccgttgga tatcccacca cgacctttcg gcgccgttag agcctcggag ggtatcggca
     7441 tgggtgaaga ccttgtgttc gtattcgtag cgtgcgcgtt cgatcatgcg ctggcgccgg
     7501 gcgcgtacgc tgtcggggtt ctgcgagcca ccctggcccg cctgggcaag gcaaaacagg
     7561 ggggcagtca aacatagggc cggtaataat ccttttacat tcatatgatt ctgcaattta
     7621 tggcatccag aaaagcttgg aggtgacgac gtctccgccg ctgatggtct ttgctgcggc
     7681 ggcccaattc accgggttgg ccgtctgctc tctcaacgga taataaaaac ggataggcat
     7741 cggaccggtt gcgcctatcg gcggattgac gacatcgata ttgggttggt ggagacgacg
     7801 gatactggtc caggaatccc aaccgcgatt ggccatggcg atccattcct ggtagccgat
     7861 cttctgtttg taatcgccgg ctgccgtagt ataggccacc gcggattgtg ccaaataagt
     7921 gctggcatca gaagcgctgc ctccccagaa cgtgatggaa gcggtgacgc cggcgttata
     7981 atattgttca gccgtattgc cgccggtgag gaaaccgcgc tcgacggctt ccgccagcag
     8041 gaaattcgtt tccgcataat ccagcacgtc acccggatag gtgggggcga tccattgagc
     8101 ggaaaaggtg gaaaacacgg agaactggtt ggttgcgccc ggtatgcctc cctggtatac
     8161 gccgccgacc gtggtaaagt ataggggtaa acgcggatcg ttccagctgt tcatcgtgtt
     8221 gatcaacaga ttggccgggc agaaatcatg acgcccgctg ttgacgatcg cctgccatac
     8281 ggggttggta ttgcccgtcg gtgacgattg gtaagcgacc gtggcattat cggaattggc
     8341 agcgaataca cctgttgcta ccgcctcctg taccttcttc ccggccgtcg tcgggtcggt
     8401 gtctgccagc accatcgcca tcttcaattt cagtgtagcc gcaaatttct tccatttggc
     8461 gatattgccc ttgtagatct ggtcggcgga gcccatcgca ggcgatgatg aattgagacc
     8521 cgcgatacag gtgtcaagcc gggtcaacag gtcggaatag actgttttgg catcatcgta
     8581 tttcggaaaa gggatgctcc ggctctccgc ctgcgagtaa gggatattgc catacgtgct
     8641 gaccagcagg ttgtacgtat agacctcgag gatatcggta atgatcaggt cattgcgcaa
     8701 ggtgccggga tcggtgacgg agaccggaaa tgcggctttc gcattcgcga agttgttgag
     8761 caccccggaa tacaggttgc cccaccagtt gtcgggagaa ttataggcac taagcacata
     8821 tttggcttcg gtcgtatact gcgtctccgt ccaggtctgt gaaaaaaccc tgaacggctc
     8881 gacgccgatg ttggtcgtag taacgtcatc ggaaagattc ttctccccgg caaggaaaag
     8941 gctggtggcg ggtaccgaca ccgcgtgttt ggtatcgatg ttcaggttct gcgtgttttt
     9001 ggtgcaggac gaggccagac caccggcaaa cagacttatg atgaatagct ttcgcattgg
     9061 aaaatgtttt aggttcattt cagatcagaa tttgactttg actttgaaac cataggtcct
     9121 gaatgtggga taagcccctg actggaatcc ctgagaaccg ttcgcgccat acgaaccact
     9181 gatgggcaca ccctgctccg gatccgcatc gggaagattc ttgtggatga tccaaaggtt
     9241 tctcccggtg agcgacagat cgagtccctt gatgaagttc gccccgctca gtatgttttg
     9301 cggcaccgac caggtcaggt tcaattctct cagcttgata tagctggcat cgtaaataaa
     9361 ggtcctggct gcctcataag caggcgagcc ccagggatag gcgacatgac cggcgacagc
     9421 cttgccattc acatccgaag cgtccaccag agtggcattg gctttgccat cggccgtcac
     9481 tccttcgaaa agataaccgc cgccctgtga cagcggagcc ctcaccccta cgccgttctt
     9541 attgtatccg cccgtatgcg tggtgagacc ggcattggcg ccgtaatcct ggtcgagcga
     9601 atacaccgaa ccgccttgct tgacatcgat caggaagccg aacgaaaggt tcttataccg
     9661 caaactgttg tagatacctc ccatccagtc cgggttgata ttcccgatgt ccgaaagccg
     9721 gttgctgctc agcacgggat acccgttggc gtcgatcagt ctcttgccct gtcccagcgt
     9781 atccacctgg ccctggccat tcaggtattt ataatcggta ccccggagga tgccgtagga
     9841 tttacccact tccgccacca gctgaacgct gttctgatac ccggtgatga ggaaggaagg
     9901 ctggccacca taaagactaa ggaccttgct cttgttggtg gaccagttga tcgtcatatc
     9961 ccattcaaag ttttgggtct tgacaggcac caggttcacc gtcagctcga cgccctggtt
    10021 ctgtacggtc ccaccattga cataaaagga ggaatacccg ctgctggtag ataccgacac
    10081 cggcatgatc tggttgacct gttgcgcatg ataataggta gcgtcgaaac ccacacgacc
    10141 ctgcaggaag gacatttccg cacccacctc atatgtattg ttgcgctccg ggaccaggct
    10201 ggggttatta ttggtcgtcg gcgaggtaaa gatcgtctgg ctgttgaagg ggtttcccgc
    10261 aacgtacgta ttgagcagac tgaacggcgg cgcatctccg cccaccgatg catagttgac
    10321 cctcagtttg ccataagtga gccattcggt ctcgggcatc aatttggaga agacccagtt
    10381 ggcagagacg gaagggtaat aatatctgtt gttggcggtt ggcaacgtcg acgatttatc
    10441 cccccggatg gtagcatcca atgtgaccat atccttccag gtcagcgtgg cgcccgcgaa
    10501 gagaccgtcc acttcttttg gaacataact ctcggtgggg gcgtttatcg gattcacgga
    10561 attgcccagt gcatagaatc ccggcacaac caggccgcca ttggtagagg cggcgataga
    10621 agtactcaga tcctgccgga tattggcgcc caacagtgct ttcaggttga gggactcgtt
    10681 gaggttcttg tcgaagttgg cgaggaagtc gtagttggtc tcggtgaagg tcgagttcgt
    10741 cctcccatag ctcggtgttc cgtaagtgcc cacattataa cgggtttcca ccagttgatt
    10801 ccagttgtcg cgggaaaccc tgcccatgat gttcaggaag ggggcgaccc tccaggtcag
    10861 cgatgcataa ccgaaataac gctggcggcc gtccgattcg aaattcgtgt aacgcaccca
    10921 ataaggattg tcatggaaag ccggtttgcc gataatgccg ggggcatttg cggtataggc
    10981 cgggacctgc cagttccagg tggcgttggt attggtccgg aaataatctg ccttcatttc
    11041 gtgcagatcg atacccgtct gccaccattg gcggaagtcg gtcataggat tgtactgcgt
    11101 accattgccg ggagcatagc cgtagccata ccggccgatc gcgtcatcct ccgtaaaact
    11161 gaaatcgcct gttgcagtga gattccgcgc aagattatac gttgccccga agaccagctg
    11221 gttcttcagc aggctgctgt tgggaagata tccgttgtcg gtgctgcggg tatatcccat
    11281 tttaaaggta cccctgtcgt tgccgccatc caggaaaaca ctggtactgt tggtgatcgg
    11341 cgtgacgaaa taatcggttg gcctgaaatg cgcggcaggt ttccatggtg tcgccttgcc
    11401 ccagttgggg tttccgggcg agaaagcatc ccattgatag accatcatgc tgggatcgta
    11461 agcagggccg gtcatctggt cgacgtcggt ttgcacgatc tgcacggcct gcccgttgct
    11521 gaaggaggta ggataccaat aaaaaaatgc gttcggatta ccggcaatgc cggcgccggc
    11581 gctgcctgag ccttccccgt attgtagctg atagctcgga agcgtgctct tgtcgggcgt
    11641 acccaccgta acgccttcat cgacgacgat acccagtcct ttgttgagtc tcgatccacg
    11701 tttggtggtg atgagtataa ccccgttgga agcaagcgag ccatacagcg cggaagcggc
    11761 cgcgcctttc aacacgctaa ccgattcgat atcgttggga ttgatatcgg atactgcgtt
    11821 gccgacatcg taaccgccgg cggagttgtt gccattatta tagggtaccc cgtccacgac
    11881 gaaaagcgcc tggttggttt gtgtcagcga tttcatcccg cggaggataa cgttgttgga
    11941 gccgcccatc gtgttgctcg aggttaccgt caacccggct accttccccg ccaggttgtc
    12001 gacaaaattg gtcgtgacca ctttgttgac ctggtcgccg ctgatctgct gcatggcata
    12061 agggaccgag ttcctggtcc ggcggatgcc gagagcggtg acgaccactt cgttcagatt
    12121 ttgggtggtg cgatgcaggg tgatagatat ctggtcctgg ctgccgacct ttatctcctg
    12181 gggttcgaat ccgaccacgg tgaccagcag gatatctgtg ctcgcggcag taatgcggaa
    12241 agtcccgtcc tcgacggtgt tgacgcccgc gtgtgtcgac cggagacgga cgctggcatt
    12301 cgggatcggg acaccgacag aatcgacgac cttgccggtg atcgttcttt tctgggcatt
    12361 ggcgttaagg aatagcaacg aagccataat caggcataac gcactgaaga gccgttttct
    12421 cattgcaggt gttgttttgg tgagtagaat agtaaagctt gcctttacga acgaatgtac
    12481 ttcaccgcaa tgcccggtac ttcatcgttt ttgatcggtt atgctcgaaa attaccaggg
    12541 tttcgcccgt cacttgcggg agcaacggga cccgccgcaa tgccccatcg ggcgtcggcc
    12601 atgcctcacc aggcttcctc tttctgaaaa gagaggatca ggatgatccc gctgagcaac
    12661 ccggaaaagc caatgaattg ctgactctcc atgcggaccc ggctgtagat gaaaagcacc
    12721 tgcgccagga ccaggaagat acaaaaagct gtgatgacga tcctgcgcag cacgcgcttg
    12781 gaagatccct taaataagcc caacagcgaa atgagggaaa agaagatacc caccagtgtt
    12841 gtcatagtgt aatggttttt tcatggatac tcggattagt atatccaata tacggccctg
    12901 ccgcgcgaat tgcaatagtt aaattgcgca gcggattttc gcaaccatcc acggaaaata
    12961 cggctgttca cgccgaaccc agcagctgcg acggcggaac accgagcgcc gcggcaagcc
    13021 ggaaaatttc tgtgatgtgg atatctttct ccccgctctc gatggatgtg atatccgcgg
    13081 gttgcaaacc gctgcgggaa gcgagttcgg tgatactcat tccctgctcc tcgcgtaatg
    13141 cagatagttg tctgccgaat gaacgcagtg cgccttcctg tttgtccata agaaaacaag
    13201 cgtttgaaga tgacaaatca atagtgagga ttggaaggac gcaaagaagg atcacccctt
    13261 ttacgggatg aacgcgaata taaccattag cagaccttct ccggcgttat tccgcgggtg
    13321 cgtttttaac cattagcaga ccccccccct ccggcgtcat tccgcgggtg cgtattcgag
    13381 gaggacggca ggatgtgctt ccagggcgac agccagctgt agaagagttg tgaaagtgat
    13441 gttgatcatc cctttctcga tcttggcgat cttgctgttg tcgatgctac aggcataaga
    13501 catctcgcgg aggctcaggc ccttgtcttt acggagtttc cgcaggttct ctccaaaccg
    13561 ttttagtatt tttttgtctg ctggcgtgat cattagttgt cagttcgtct gatgaatacc
    13621 taaggtggaa gattttttga aaatctttgg ggtcgtataa ggccgcaaat aaaatttaat
    13681 aattcgttca tcaggggcgc gtcgccaccg caatcttcga atcagcagta ccgtaataca
    13741 acatccaccg gccgtcaaac ctggccagtc cttcaataaa acaaacggag ctaacctgtc
    13801 ccgttagctc atagggctta tccgggcgca ggaaataatg atcgagacga tgcagtaaat
    13861 gggtcggatc ggcactatcg aataaggctt ccgatgcggt ataggtgcct tccggcaatg
    13921 ccggatcgcc gatggagggg atattccggc tattgtaaag aagaagaatg ccctgatccg
    13981 tgagcatggc gggaggtccg gattccacga ggtcactgtc gaacttccct ttccgggtcg
    14041 gcaggacgat cttcaaggca ggcatctccc gcgcctgccc cctcaactca acgggatcgg
    14101 tctggcccgg ggccttctcc acgggtgtcc agtcgatgag attatcgctg actgccgccc
    14161 agatgaactg atcgccccaa tacatccagt acttgccctt gatccgcgcg gcgacgaccc
    14221 tcgcgccctc atatctcgat acgatactcc ctgatttcga ccatttgtcg agatactttc
    14281 catgcatggc cttaccaaaa gcgggaccat gcttagtcca gtggaccagg tctgtcgacg
    14341 tagcgaccag tagccttgcg gtatgtccgt caaaaccggt ataagtcatg taatacaccc
    14401 ccttcccatc ctccactacg cgcggatcct cgcatccgcc ttcccattcg tatttccgga
    14461 aggcgtcgtt gtcgggatat agtacaggca ttgcccggcg ggtaaaatga tatccgtcta
    14521 tactttcggc aagaccgatc cgggaggtgc cggcggggtg cccgacggcg tcctgcgcgc
    14581 ggtataacag gtagagctta ccgttccgca ctacagtagc gggattaaaa acgtctttcg
    14641 cttcccaggc aatggtccga tgaaggacgg ggtcggtgaa gatacctgtg ccgggtgtga
    14701 gcacggggtt tatgctgtcc gattttataa agcccgtaag ggcccaggcg gggtcgccgg
    14761 acgaaaaaac agctgtaacc aggaagccgg cgattagcgc cggaaagacg attttcatgc
    14821 ttccctaaaa ataagaagct aatcattcat accaatcata aatttgattg ccgatgatgt
    14881 ccaccatgat cgcgcatttt ggggaatctg tttcccaatc cgcgcccctt cttttgataa
    14941 ggccgcaata gacgttattg aggatcacct ggaaagtggt gggaatacgg tgctggatgc
    15001 ctgcctgcag gggccaaatc actcctttgt attgtcttcc atcgtagttg aaatggatct
    15061 gaaacttatt gaacatacac tagggtttcc taatccagat caaattattg tgccagaaaa
    15121 aagcacgtgg catggggctt atccgacccg cgggaattcg atcccggtta ttttccgata
    15181 aatgtccacg gcatagaggt cggtcatgcc ggcgatgaag tcgaccaccg acagcagatc
    15241 ggtataaaga ctttcggacg aaccggaaac gatgaactgc tccgggatca attgcaggag
    15301 cttaatcgat tttgcagaac gcgggttcag tacggcgccc atgaattccc tcaataatcc
    15361 gccgatcaca tgataacccg cgatctcgat ctcgaccacc gaccggtggt tatagatatg
    15421 cctgacggaa taggcatcga tcgtcctgat cagggcggct tcgcgcgcgg gcagaaaatc
    15481 gatcagcggc cgggaaaccg ttccttccag caagtccgct tccttctcca taaagatgtc
    15541 ccttacgcgg ctgatcagga gatttatcag tttggcacgg aggaattgca ctttctggtt
    15601 gtcgtcacgg ataccgcgga atacttcctc gaccctatcc cgggcatcgt agcctgtctc
    15661 accgtcgaaa aaagaaagaa aaagatcgcg gatggtgtcg atggaaatta tgttgagccg
    15721 atgggcatct tcgaaatcga tgatgcggta gcagatatca tcggcagcct ccaccaggta
    15781 aacgaaagga tggcggagaa atacattttt c
//
LOCUS       DASYSV010000082         4128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_916144, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000082 DASYSV010000000
VERSION     DASYSV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916144"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /gene="hutU"
                     /locus_tag="VGQ51_02530"
     CDS             <1..875
                     /gene="hutU"
                     /locus_tag="VGQ51_02530"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEV9035462.1"
                     /translation="HDPLIGYWPHELTYDQAVVLRRDNPTQYIDYAYRSIRRHVECML
                     ELQRRGAITFDYGNNIRARARERGLENAFDFPGFVPAYIRPLFCEGKGPFRWAALSGD
                     PADIAVTDGVIASLFPENSSLQRWLKLAREKIAFQGLPARICWLGQGEREKAGLAFNE
                     LVRSGAVKAPIVIGRDHLDTGSVASPNRETEAMLDGSDAVADWPILNALVNTAGGASW
                     VSLHHGGGVGMGYSIHAGMVIVADGTPEAAARLSRVLRNDPGMGVIRHADAGYQLAQD
                     TARSHDLDIRARLK"
     gene            1189..1887
                     /locus_tag="VGQ51_02535"
     CDS             1189..1887
                     /locus_tag="VGQ51_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEV9035463.1"
                     /translation="MLLYLPFSGNIADSSGNGNPTTAVGSVLTADAHGYANSAFGATG
                     NGERIYVTNNGSIQFDTAYTVSFGFMVNAARQQFYMSMVDPATGYGPSFALGTSLTAN
                     SNLVMGTEDVSLGCGKYGLADNINVTDTSNFVPLVGSWYSFIGVYHKGSVQIYINGKM
                     ISSKTGTGTSALLCPASQVIIGAWWNSDPLNLNGKLDNIRLYNRVLTPHEIAALSSSY
                     QVTSNSQKHQLITH"
     gene            1901..2851
                     /locus_tag="VGQ51_02540"
     CDS             1901..2851
                     /locus_tag="VGQ51_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEV9035464.1"
                     /translation="MTFPKSLFIAAVLVPGTLALIVGCDKNATPPPPVHDTVTVIKYD
                     TSILIQKIDTPDLKTGLVLYLPFNGSFADSSGLNNTVTPINGAALGYDMHGYANSAFN
                     SIGNGTVLQVTNNGSYAVDTAFSLSFDFKINTNSVYYGGYDYSGMMVFASIVNFSNGL
                     GPTFDCGLDVPSEPMNFDFGVTGSANTCSGYGTIDAARNNDSSNFVPQVGAWYNAICI
                     FSHGNSSIYINGKLVSQKTGLSSSVNFCPDASFIVGGWWNGTSGGASALENVRGEMDE
                     LRFYNRTLNAKQIAWLARNFQVNSTKQGATPETGKGSRLN"
     gene            2965..>4128
                     /locus_tag="VGQ51_02545"
     CDS             2965..>4128
                     /locus_tag="VGQ51_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEV9035465.1"
                     /translation="MGRLIIISNRLPYSLDKEGDKVSLRQSSGGLVSAIKSYFENTAA
                     QKGEITEKMWMGVADFPQEDWDQMAADGSRQDFNVLPVFVDKDLYHDYYNGFSNSVLW
                     PLFHYFTSLAEYKNEYFEAYVKVNQQFAERLVPLLQPDDQIWIHDYQLLILPHLIRTK
                     RPEATIGFFLHIPFPSYEIFRLMPTEWKKTLLHGVMGADLIGFHTYDYVQHFLQSVKM
                     LLGADSHFHTLQYMNRLIRIDLFPIGIDYNKFHNGGNDPEVEEYRNKVRTNLEDKKII
                     FSVDRLDYTKGLMQRLEGFEYFLERYPEWREKMVFILNIVPSRDTIPAYNDRKKQIEE
                     KVGTINGRFSTISWQPLIYRYNHLSFPELIALYQAADVALITPLRDGMNLVSKE"
BASE COUNT          969 a         1079 c         1119 g          961 t
ORIGIN      
        1 cacacgaccc gctgatcgga tactggccgc atgagctgac gtacgaccag gcggttgtcc
       61 tgcgcaggga caatccgacg caatatatcg attatgccta ccggagtatc cgccggcatg
      121 tggaatgcat gctcgaattg cagcgtcggg gtgcgataac cttcgactat ggcaacaata
      181 tacgggcgag ggcgcgcgaa agaggactgg agaatgcgtt cgatttcccg ggctttgtcc
      241 cggcctatat acgcccgctt ttttgcgagg ggaagggccc attccggtgg gccgcgctca
      301 gcggcgaccc ggccgatata gccgtcacgg atggggtgat cgcctcgctg tttcctgaaa
      361 actcgtcgct gcaacggtgg ttgaaattgg cgcgcgaaaa gatcgctttt caggggctgc
      421 ccgcgcgtat ctgctggttg ggccagggcg aaagggaaaa ggctggcctg gcattcaatg
      481 aactggtgcg atcgggtgcc gttaaagcgc cgatcgtgat cggtcgggac cacctggaca
      541 caggcagcgt cgccagcccg aaccgcgaga cggaagccat gctggacggg agcgatgccg
      601 ttgcagactg gccgatcctg aatgccctgg tcaatacggc gggtggcgcc agctgggtca
      661 gcctgcatca tggcggaggc gtgggaatgg gatatagcat tcatgccggg atggtcatcg
      721 tggccgatgg gacgcccgaa gcggctgcac gactgagccg ggtattgcgc aatgaccccg
      781 gcatgggggt aatccgccat gcagacgctg ggtatcagct ggcacaggac accgccagaa
      841 gccatgatct ggacatcagg gcaagattaa aataatggtg cgctgcacag ttattgccgt
      901 tttcgacacg cttgtagccg gaacgacaca cttgtgaaat atgcttgcgt ttagaagggg
      961 agtttattac tttgcactcg ttaaaattca ttaacccaga cttaccgtta tgaaaatcaa
     1021 aagattaacc cccctctttt aatcgtcctt cccgccctga tcttcgctac cggttgtaac
     1081 aagaatgtca cgccgccacc tcccgtccat gatacgacag tagtcgtcaa aacggacacc
     1141 atacaggttc ctcctccgcc agacccgacg gtcaatctta ctaaaggttt gctactctat
     1201 ctgccgttct ccggtaatat cgccgactcc agtggcaacg ggaatcccac gacggcggta
     1261 gggtccgtac tgactgctga cgcgcatggg tatgccaaca gtgctttcgg cgctacaggc
     1321 aatggcgaaa ggatatacgt gaccaataac ggatcgatcc aattcgatac ggcctatacg
     1381 gtttcgtttg gtttcatggt caatgcagcg cggcagcaat tctatatgag catggttgac
     1441 ccggctacag gttatggacc cagcttcgcg ttgggaactt ctttgacagc caacagcaac
     1501 ctggtcatgg gaaccgagga tgtatcgctg ggctgcggta aatacggact tgccgataac
     1561 attaatgtca ccgatacttc caattttgtg cctttggttg gcagctggta tagtttcatc
     1621 ggcgtctacc ataaaggctc cgttcaaata tatattaatg gaaaaatgat ttcgagcaag
     1681 actggtacag gcacaagcgc actcttatgt cctgcatccc aggtgatcat cggggcatgg
     1741 tggaattcag accctcttaa cctgaacggg aagctcgaca atatccggct gtacaaccgc
     1801 gttctgacgc cgcatgagat cgccgcatta tcgagcagtt accaggttac ttccaacagc
     1861 caaaaacacc agttaattac acattaattt taataagatt atgaccttcc ctaaatctct
     1921 gttcatcgct gctgtgctgg taccgggcac actcgcactg atcgtcgggt gcgataaaaa
     1981 tgctactcct ccgccgccgg tccatgacac ggtaacggta atcaaatacg acacgtccat
     2041 cctcattcag aagatcgata cgccggatct gaagaccggg ttggtactgt atctgccttt
     2101 caatggcagc ttcgccgata gcagcgggct caacaatacg gtaaccccga tcaacggcgc
     2161 ggcgctgggc tatgacatgc acgggtatgc gaacagcgcc ttcaattcca tcggcaacgg
     2221 tacggtgctg caggtcacca acaacggcag ttatgccgtg gatacggcgt tctcgctttc
     2281 cttcgatttc aagatcaaca cgaattcggt ctattatgga ggatatgact atagcgggat
     2341 gatggtgttc gcgagcatcg tgaacttcag caacggtctg ggcccgacct tcgattgcgg
     2401 tctggacgtc ccgagtgagc cgatgaattt cgatttcggc gttaccggct cagccaacac
     2461 ctgttcgggt tacgggacga tagatgcggc cagaaataac gactcttcca acttcgttcc
     2521 gcaggtcggc gcatggtaca acgcgatctg tatcttttca cacggcaaca gttccattta
     2581 tatcaacgga aagctggtga gccaaaagac cgggcttagc agttcggtca atttctgtcc
     2641 cgatgccagc tttatcgtcg gtggctggtg gaatggtacc tccggtgggg cctctgccct
     2701 cgagaatgtc agaggagaga tggacgagct gaggttctat aaccgcacgc tgaatgccaa
     2761 acagatcgcc tggttggcca ggaatttcca ggtgaactcg accaaacagg gtgcgacccc
     2821 ggagacgggc aaaggttcgc gtctcaacta gactaacgaa ggctcgtatt cgcattatgt
     2881 tctatagcta aaaggaaccc cggtccgggg ttcttttttt aacgtttcga tcccattttg
     2941 gcgctattgt tgaggatgga gggcatggga cgtttgatca tcatttccaa ccggcttcct
     3001 tactccctgg acaaggaagg cgataaggtc agtttgaggc aaagttcggg ggggcttgtt
     3061 tcggccatca aaagctactt cgagaacact gcggcccaga agggggagat cacggagaaa
     3121 atgtggatgg gtgtggcgga ttttccccag gaggactggg atcagatggc tgcagacggg
     3181 tcgcggcaag atttcaatgt gctgccggta ttcgtcgaca aggatctgta tcacgactat
     3241 tacaatgggt tttccaattc ggtcctctgg ccgctgttcc attattttac ttcactggct
     3301 gaatataaga acgaatattt cgaggcgtat gtaaaagtga accaacagtt tgccgagagg
     3361 ctcgtcccgc tgttgcaacc cgatgaccag atctggatac acgactatca gctcctgatc
     3421 ctgccgcacc tcatccggac caagcgtcct gaggccacca tcggattctt tctacatatc
     3481 cctttcccct cctacgagat cttccggcta atgcctaccg agtggaaaaa gaccctgctg
     3541 catggtgtga tgggcgcgga cctgatcggt tttcacacct acgactacgt gcagcatttc
     3601 ctgcaatcgg tgaagatgct gctgggcgcc gacagtcatt tccataccct gcagtatatg
     3661 aacaggctga tccggatcga cctgttcccg ataggtatcg attataacaa attccataac
     3721 ggtggcaacg atcccgaggt agaagaatac cgcaataagg tgcgcaccaa tctcgaggac
     3781 aagaagatca ttttctccgt cgaccgtctc gactatacga agggattgat gcagcgtctc
     3841 gagggtttcg aatattttct cgagcgttat cccgagtggc gtgaaaagat ggtgttcatc
     3901 ctgaatatcg ttcccagccg ggatacgata cctgcctata atgaccgaaa aaaacagatc
     3961 gaggagaagg tcgggacgat caatggccgt ttctccacca ttagctggca gccgctgatc
     4021 tacaggtata accatctttc atttccggag ctgatcgctt tataccaggc cgctgacgtc
     4081 gccctgatca cgcccttgcg tgacgggatg aatctcgtct cgaaggaa
//
LOCUS       DASYSV010000083         4649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1042248, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000083 DASYSV010000000
VERSION     DASYSV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042248"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="hutU"
                     /locus_tag="VGQ51_02550"
     CDS             complement(<1..811)
                     /gene="hutU"
                     /locus_tag="VGQ51_02550"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEV9035466.1"
                     /translation="MSTVSYQRYDPVKYKTPTGTKLTCKGWVQEAALRMLLNNLNPEV
                     AERPDELIVYGGRGKAARNFESLDLIIRALKDLGEDETLLIQSGKPVGILRTHRDAPR
                     VLLSNSQLVPKWADWEHFDELEKKGLMMYGQMTAGSWIYIGSQGIVQGTYETYAAAAA
                     KHFSSTLRGTLNVTAGLGGMGGAQPLAITMNGGVALIAEVEEWRIDKRIETRYLDRKT
                     EDIDAAIDLALAARERGEAVSIGVHCNAVHLLERLLERNIVPDTLTDQTSAH"
     gene            complement(830..2401)
                     /gene="hutH"
                     /locus_tag="VGQ51_02555"
     CDS             complement(830..2401)
                     /gene="hutH"
                     /locus_tag="VGQ51_02555"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEV9035467.1"
                     /translation="MDQQNSFHFGSDRLDIGRTIRLGEGKVQGSLSPEVVEKINASQR
                     HVTDIVSRQSTVYGVNTGFGILANTRISEEDTQILQHKILQSHSVGVGEPVPTDIARL
                     MLITKVQALAQGYSGVPRTTLERILWHIREDIIPVVPEKGSVGASGDLAPLSHLFLPL
                     IGLGEVFYNGKRVPSKEVLDRYGLSPLRLGPKEGLALINGTQFILAYAVKGVQRLHYC
                     LEAADIIGAMSLEALTGTKAPFDERLHRLRPFAGNRLVAQRLRLLLHDSEIMKSHVDC
                     GRVQDPYSLRCMPQVHGASRNAWLHLRQLTETELNAVTDNPIILGEGDTISGGNFHGQ
                     PLALPLDYACLAAAEIGNISDRRCYLLLEGKWGLPLLLMRNVGLNSGFMIPQYTTAAL
                     VTENKTLCFPASADSIPTSLGQEDHVSMGSISGRKLWQVLDNLEYILAIELMSACQAI
                     EFRRPLKSSGILEAAHEYVRNFVGFASEDRIFAEDIRQLHRIIHDFSFVGHCNEFALR
                     KGIDWNAGFEAEFSI"
     gene            complement(2469..3215)
                     /locus_tag="VGQ51_02560"
     CDS             complement(2469..3215)
                     /locus_tag="VGQ51_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9035468.1"
                     /translation="MIRTVLIDDEPDSIRILQRLLETYCPEVSVIGKAEGVESGEQTI
                     RTLVPDLVFLDIEMIQGNAFDLLNRLQPQTFQVIFVTAFDNYAIRAFKYSAVDYLLKP
                     VDVEDLRSAVGKVAGRSKDKIDMLQIKTLLENVGNLHIWQQKMAVPTMTGLNFITISE
                     IIRFEARGNYTGIHLDNGECVLATKNIQDYDALLPDTLFYRIHHSHIINLQKIHKYQK
                     GRGGYIIMEDGTSIEVASRRREDFLRRLIK"
     gene            complement(3212..>4649)
                     /locus_tag="VGQ51_02565"
     CDS             complement(3212..>4649)
                     /locus_tag="VGQ51_02565"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9035469.1"
                     /translation="GTIWIAFYDGGVVAYRDGRVITTLTTKQGLTSNICRTLFVQDFA
                     VWVGTNKGINKIALDRPGYPVTRYTSNDGLGSDNVNSIYAVGPTIYVGTSGGLSYFDQ
                     TKVNVSEGTQLHLLSILNSGTERIADSGHLVLPYKDKSIRLEYVAISYRSVGNILYRY
                     RVLGLDTAWRETKETFLEYPSLPSGDYVFQMVAVNKFGVASSLLSVPFEVATPFWHTP
                     IFDGALIAIFVSMVWFVATLRIKRIRRRQEEKEQINHRLAELENTALQAQMNPHFIFN
                     CLNSIQQYIFDHDTLVANKYLTGFSRLIRATLHNSSRNFISLADEIDYLSTYLSLEKL
                     RFKEKMDYTIEVDPSIDKDLFIIPPMLIQPFAENSMRHGLRHKIEGRGTIHIRMKTSD
                     EKLLVTVEDNGIGRKKAASYKTHEHIEYQSKGMSLTADRIRMMNVKYGDSIHIEVTDL
                     EDADGRVAGTRVSLEFPIFHLATQNVII"
BASE COUNT         1008 a         1317 c         1209 g         1115 t
ORIGIN      
        1 cgtgtgccga ggtctggtct gtcaatgtat cggggacaat gttccgctcc agcagtcgct
       61 ccagcagatg caccgcattg cagtggacac cgatagacac ggcctcacct ctttcgcgcg
      121 cagccaatgc cagatcgatc gcggcatcga tatcctccgt cttccggtcc aggtaccttg
      181 tctctatccg tttgtcgata cgccattctt cgacctccgc gatcagcgca acgccaccgt
      241 tcatcgtaat ggccaggggc tgagcgcctc ccattccccc gagcccagcc gtaacgttga
      301 gcgtgccgcg cagcgtggat gaaaaatgtt tcgcagcggc ggcggcatag gtttcatagg
      361 taccctgcac gatgccttgc gagccgatat agatccagga ccccgccgtc atctggccgt
      421 acatcatgag cccctttttt tccagctcgt cgaaatgctc ccagtcggcc catttgggca
      481 ccagctgaga attggagagt aatacacgag gggcgtcgcg atgcgtacgc aggatgccca
      541 ccggttttcc gctctggatc aataaggtct cgtcttcccc taagtctttc agcgcgcgaa
      601 tgatgagatc caagctctcg aagttgcgtg cggccttgcc gcgtccgcca tacacgatca
      661 gctcatcggg acgctcggca acttcggggt tcaggttgtt cagcaacatc ctcaatgccg
      721 cctcctgcac ccagccttta caggtcagtt tcgttcctgt aggagtttta tacttcacgg
      781 ggtcataacg ttgataggaa accgtactca taagatccga tttgaaaggt taaatgctga
      841 attctgcttc gaaaccggca ttccaatcga tccccttgcg caatgcaaat tcattgcagt
      901 gaccaacgaa ggagaaatcg tggatgatac ggtgcaactg ccggatatct tctgcaaaga
      961 tgcggtcttc actcgcaaat ccgacgaaat ttcttacata ttcatgcgcc gcctccagga
     1021 tgccgctgct tttcaggggt cgccggaatt cgatcgcctg gcaggcgctc atcagttcga
     1081 tggccagtat gtattcgaga ttgtccagca cctgccataa ttttcttccg ctgatgctgc
     1141 ccatgctgac atgatcttcc tggcccagcg aggtcggtat gctgtctgcg cttgcgggaa
     1201 aacaaagcgt tttattttcc gtgaccagcg cggcggtggt atactgtggt atcatgaacc
     1261 cgctgttcag acccacgttg cgcatcagca gcaatggcag gccccatttc ccttccaaca
     1321 aaagataaca acgccggtcg gagatattcc cgatctcggc ggccgccaaa caggcatagt
     1381 ccagcggaag agccaggggt tggccatgga aattgccgcc gctgatcgta tctccctcgc
     1441 ccaatatgat gggattatcg gtgactgcgt tcaattccgt ctcggtcagt tgccggagat
     1501 gcaaccaggc attccgcgag gcgccatgca cctgcggcat acaacggagt gaataagggt
     1561 cctgtacccg cccgcaatcc acatggcttt tcatgatctc actatcgtgc agcaacaaac
     1621 gcagccgttg agccaccagc cggttgccgg caaagggtcg cagccggtgt agccgctcgt
     1681 cgaaaggggc cttggttccc gtaagcgcct ccaggctcat ggcgcctata atgtcagctg
     1741 cctcgagaca ataatgcagc cgctgaactc ctttcacggc ataggcaagg atgaattgag
     1801 taccattgat caacgccaac ccctctttgg gcccaagacg cagcggggat agtccgtacc
     1861 ggtccagtac ttccttggaa gggacccgct ttccgttgta aaacacctcc ccaagtccga
     1921 taagtggaag gaaaagatgc gacagcggcg ccagatcgcc cgaggcgccc accgaaccct
     1981 tttcagggac gacaggaatg atgtcttcgc ggatatgcca gagtatccgc tccagcgtcg
     2041 tgcggggcac cccagaatat ccctgcgcca gggcctggac cttggtgatc agcatcagcc
     2101 gggcgatgtc tgttggaacg ggttcaccta cccctacact atgactttga aggatcttat
     2161 gctgcaggat ctgcgtgtcc tcctcggata tccgggtatt ggccaggatg ccgaacccgg
     2221 tattgacccc gtatacagtg gactggcggc taacgatgtc ggtaacatgc cgctggctgg
     2281 cgtttatctt ttcgacgacc tcgggcgaca aactcccctg taccttacct tccccgagcc
     2341 tgatcgtcct gccgatgtcg agtctgtccg atccgaaatg gaaactgttt tgctgatcca
     2401 tgacaagctt ttccgcgcaa aaataggctt tttgcccact cgatcgctgc ctgaccccgg
     2461 cttgatcctt acttgatcag ccgacggaga aaatcctctc tgcgacggct ggccacttcg
     2521 atagaggttc cgtcctccat gatgatatac cctcccctgc ctttctggta tttgtgtatc
     2581 ttctgcagat tgatgatatg cgaatgatgt atccggtaga atagcgtatc cggtaacagc
     2641 gcgtcgtaat cctggatgtt cttggtcgca agaacgcatt ccccgttgtc gagatggatg
     2701 cccgtgtaat ttccacgggc ttcaaaccgg atgatctcgc tgatggtaat gaagttcaga
     2761 ccggtcattg tcggaactgc catcttctgc tgccagatgt ggaggttccc gacattctcc
     2821 agcagggtct tgatctggag catgtcgatc ttgtccttgc tgcggcctgc cactttgccc
     2881 acggcgctgc gcagatcttc gacatccacc ggcttcagca ggtagtccac tgcgctgtat
     2941 ttgaatgccc gaattgcata gttatcgaag gcagtcacga agatgacctg gaaggtctgc
     3001 ggctgcaacc ggttgagcag atcgaacgca ttcccctgga tcatttcgat atccaggaat
     3061 accaggtcgg gaaccaaggt acggatggtc tgctcaccgg actctacccc ttcggccttt
     3121 ccgatcacgc tcacctcggg acaataggtc tcgaggagac gctggaggat ccttatgcta
     3181 tcgggttcgt catcgattag gactgttctg atcatatgat tacattttgg gtagccaggt
     3241 gaaagatcgg gaattccagc gacactcgcg tacccgcgac ccggccatcg gcgtcctcca
     3301 ggtccgtcac ttcgatatgg atgctgtccc catattttac attcatcatc ctgatacggt
     3361 cggccgtcag cgacattccc ttcgattgat actcgatatg ttcgtgtgtc ttgtacgaag
     3421 cggctttctt tcttccgata ccattgtctt cgacggtgac cagtagcttt tcgtccgatg
     3481 tcttcatacg gatatgaata gttcccctgc cttcgatctt atgacgcagg ccgtgccgca
     3541 tgctgttttc cgcgaagggc tggatcagca tcggcggaat gataaagaga tctttatcga
     3601 tggagggatc gacctcgata gtataatcca ttttttcctt gaaccgcaat ttttccagcg
     3661 aaagataggt ggacagataa tcgatctcgt ccgccaggga tatgaaattc ctcgaagagt
     3721 tgtgcagggt cgcccggatc agcctcgaaa atccggtgag gtatttgttg gcgacaaggg
     3781 tatcgtgatc gaagatgtat tgctgaatgg aattgaggca attgaagata aaatgagggt
     3841 tcatctgggc ctgcaatgcc gtattctcca attccgccag ccggtgattg atctgctcct
     3901 tttcttcctg acggcggcgg atgcgtttga tcctcagggt cgcgacaaac cagaccatcg
     3961 agacgaagat tgcgatcagg gcgccatcga aaattggcgt atgccagaag ggagtagcca
     4021 cctcgaaagg caccgacaac agcgaactgg cgacaccgaa cttattgacg gccaccattt
     4081 gaaaaacata atcacccgac ggaagcgagg gatattccaa aaaggtctcc tttgtctccc
     4141 gccaggccgt gtccagcccc agcacccggt aacggtacag gatattcccg acgctccggt
     4201 aggagatcgc gacatattcg agacggatgc tcttatcttt atacggtaat accaggtggc
     4261 ccgaatccgc gatccgttcc gtcccggagt tgagaatgga caacagatgc agctgggtcc
     4321 cttccgatac gttgaccttt gtctgatcaa aatagctgag accgccactg gtacccacgt
     4381 agatggtagg accaacggca tagatcgagt tgacattatc cgaacccaat ccgtcattgg
     4441 atgtataccg ggtgactgga taccccggcc ggtcgagcgc gatcttattg atccctttat
     4501 tggtcccgac ccacaccgca aagtcctgga caaacagtgt cctgcagata ttgctggtaa
     4561 gtccctgttt cgtggtcagg gtcgtgatca cccgaccatc cctgtatgcg acgacgccgc
     4621 cgtcatagaa ggcgatccag atcgttccg
//
LOCUS       DASYSV010000084         5920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1940623, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000084 DASYSV010000000
VERSION     DASYSV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940623"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VGQ51_02570"
     CDS             <1..461
                     /locus_tag="VGQ51_02570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEV9035470.1"
                     /translation="LARKNSLWPMPFATSCCGIEFMATMASHYDLARFGAERLGFSPR
                     QCDLLMVIGTIAKKMGPIVKQVYLQMAEPRWVMAVGACASSGGIFDSYSVLQGIDQVV
                     PVDVYVPGCPPRPEAILDGFMRIQDLVGKESIRRRNGDRYKELLQSYGIQ"
     gene            466..1107
                     /locus_tag="VGQ51_02575"
     CDS             466..1107
                     /locus_tag="VGQ51_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEV9035471.1"
                     /translation="MALTNETIKERLIAKFADQLSHFEEPYGLLTFEAPKELNLKVLQ
                     FLFDDEELRFRFLTDLTAVHYPGGVGRELAVVYHLHNLVDNIRLRFKVFTSIGQPDVF
                     TATQIYSSANWMERETYDFFGVNFVGHPNLKRILNVDEMDYFPMRKEYPMEDQTRVDK
                     DDAMFGRGDLPAAASAGAVETPRSASEPTVPASGTPGEPGQKQDWPSDTTKIS"
     gene            1107..2333
                     /locus_tag="VGQ51_02580"
     CDS             1107..2333
                     /locus_tag="VGQ51_02580"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEV9035472.1"
                     /translation="MSTEHHIKLPEGSIEKTTTTLNLGPTHPATHGVFQNIVELDGER
                     IVSAEQTVGYIHRAFEKIAERRPLYQITTLTDRLNYCSSPINNMGWHMTVEKLLGLKL
                     PKRVDYLRVIIMELARISDHLICNSVVGVDSGAFTGFLYVMQYRELIYEIYEEICGSR
                     LTTNMGRIGGFERNFSNAAWEKLEKFMAEYPAVLKEFENLFTRNRIFMERTIGAGPIS
                     GDRALNYGFTGPNLRAAGVDYDVRVHSPYSSYEDFDFEIPVGSTGDCYDRFLVREGEM
                     WQSMRIIEQAYRKIQDMKGADADVYHADAPEYYLPKKEDVYTKMEALIWHFKIVMGET
                     QIPPGEVYHSVEGGNGELGFYLISDGGRNPYRLHFRRPCFIYYQAYSELTQGAMLSDA
                     IIVMSSLNLIAGEMDA"
     gene            2338..2838
                     /locus_tag="VGQ51_02585"
     CDS             2338..2838
                     /locus_tag="VGQ51_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEV9035473.1"
                     /translation="MIRFSDEKLKKVDEIVARYPAGKQKSAVIPVLHMAQEEFGGWLS
                     VDAMDYVAGLLKLEPIEVYEVATFYSMLNTKPVGRFVFEVCQTGPCMVRGCDDIIGHI
                     KTRLGIGVGETTADGLFTLKTVECLGACGYAPMMQLGKYFREHLTKEKVDAIIKECRE
                     KAAVNN"
     gene            2844..3020
                     /locus_tag="VGQ51_02590"
     CDS             2844..3020
                     /locus_tag="VGQ51_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035474.1"
                     /translation="MKRKKFRIITFSLLAAGLIPMIASMGIEKESMKELLLGVTGLLY
                     LAAAIQIVIYKRNG"
     gene            3013..4368
                     /gene="nuoF"
                     /locus_tag="VGQ51_02595"
     CDS             3013..4368
                     /gene="nuoF"
                     /locus_tag="VGQ51_02595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEV9035475.1"
                     /translation="MGRKLLLDKANVEGIRGYDVYRREGGYASVEKALKTMTPDQVTE
                     EVKKSGLRGRGGAGFPTGMKWSFLAKPEGVPRYLVCNADESEPGTFKDRYLMEFLPHL
                     LIEGLIVSSFALGSKSTYIYIRGEYAWIPEILEQAIAEAKANGWLGKNILGTGFDCEI
                     YVHTGAGAYICGEETALIESLEGKRGNPRIKPPFPAVKGVWDCPTVVNNVETLAAVVP
                     IINNGGEAYSKIGVGKSTGTKLISACGNINKPGVYEIEMTMSVEEFIYSDEFCGGIPN
                     GKRLKACIPGGSSVPILPANLLLKTVKGETRLMNYESLSDGGFAKGSMMGSGGFIVLD
                     EDQCIVRNTYTLARFYRHESCGQCSPCREGTGWMEKILRNIDTGKGKKSDIDLLWDIQ
                     RKIEGNTICPLGDAAAWPVAAAIRHFRDEFEWHVDNPQECLVRNYGLAHYADPLEAAT
                     A"
     gene            4380..5534
                     /locus_tag="VGQ51_02600"
     CDS             4380..5534
                     /locus_tag="VGQ51_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV9035476.1"
                     /translation="MADEKKLFKVTIDNITIEVEPGTSILQAARRIGGDVAPPAMCYY
                     TKLKGSGGKCRTCLVEVAAGSTADPRPMPKLIASCRTNVMDGMVVKNITSEKVIDARN
                     GVVEFLLINHPLDCPICDQAGECHLQDLSYEHGKEGTRYEFTRRTFEKHDLGPYIQLH
                     MTRCILCYRCVFVADQITERREHGVLDRGDQSEIATFIQKSLDHDFIGNVIDVCPVGA
                     LTDKTFRFKNRVWFLKPVDAHRDCNHPKCKGNVTLWYRGEEVFRVTARKDQWGEVEDW
                     ICNECRFEKKKTSDWVIEGPTKVSRHSVISQGHYVGVNRPQEVLYDVLDGRKPRLLMD
                     IHSVSEVNRPEIDLSKIEGPAHSTDFEPHEPRGTTGVEAGNPWDTGKLEK"
     gene            5546..>5920
                     /locus_tag="VGQ51_02605"
     CDS             5546..>5920
                     /locus_tag="VGQ51_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEV9035477.1"
                     /translation="MTLLAIDMVLLLEKAILILIIISVSLLVAMYETWAERKVAAFMQ
                     DRRGPNRAGPAGLFQPLADGLKLFMKEEIIPLSSNRFLFVLGPGLAMLTAMMTSAVVP
                     WSSHFTIGGREVSLQIADVNIGI"
BASE COUNT         1400 a         1321 c         1909 g         1290 t
ORIGIN      
        1 gattagcccg taagaactcg ttgtggccca tgcctttcgc aacgtcctgt tgcggtatcg
       61 aattcatggc cacgatggcc agtcactacg atctggcgag atttggcgcc gagcgtctcg
      121 ggttttcgcc acgtcagtgc gatctgctga tggtgatcgg caccatcgcc aagaaaatgg
      181 gccctattgt caaacaagtg tatctgcaga tggccgagcc gcgttgggtg atggcagttg
      241 gtgcctgtgc cagcagcggg ggaattttcg attcttattc ggtattacag gggatcgacc
      301 aggtggtccc tgtagacgtt tatgtgccgg gttgcccgcc gagaccggaa gcgatactcg
      361 atgggtttat gcgcatccag gacctggtag gaaaggaaag tatcaggcgc cggaatggcg
      421 atcgctataa agaactactc cagagctacg gaatacaata atattatggc tttaacgaat
      481 gaaacgatca aggaacgcct aattgccaag tttgccgacc aactgtccca tttcgaagag
      541 ccgtacggtc tgctgacctt tgaggcgccg aaggaattga atttgaaagt attgcagttc
      601 ttatttgacg atgaggagct gcggttccgg tttctcaccg atctgacggc tgtgcattat
      661 cccggcgggg tcggccgcga gctggcggta gtctatcacc ttcataatct cgtcgataat
      721 atccgtcttc gtttcaaggt ctttacctcc atcggacagc ccgatgtctt taccgccacg
      781 caaatttatt caagcgccaa ctggatggaa agggagacct atgatttctt cggcgtcaac
      841 tttgtcgggc acccgaatct gaaacgcatc ctgaatgtcg atgagatgga ttatttcccc
      901 atgcggaagg aatacccgat ggaagatcag acgcgcgtgg acaaggacga tgcgatgttt
      961 ggccggggtg atctgccggc ggcagcgagt gcaggggcgg tagagacacc gaggtcggct
     1021 tcggagccga cggtgcccgc aagcgggacg cccggagagc cgggacagaa acaagattgg
     1081 ccaagcgata cgacaaaaat aagctaatga gtacggaaca tcatatcaag cttccggaag
     1141 gcagcatcga gaaaactact actacgttga acctggggcc tacgcacccg gcgacacacg
     1201 gagttttcca gaatatcgtg gagctggacg gggagcggat cgtctcggcg gagcagacgg
     1261 tgggatatat tcaccgggct ttcgagaaga tcgccgaacg gcggccgtta taccagatca
     1321 cgacgctgac ggaccggctg aactattgtt cttccccgat caacaatatg ggctggcata
     1381 tgaccgtcga gaagctgctg gggttgaaat tgcccaaacg cgtggattat ctgagggtaa
     1441 ttatcatgga gctggcgcgt atctcggacc acctgatctg taattcggtg gtgggtgtgg
     1501 acagcggggc ctttacgggc ttcctgtatg taatgcaata ccgcgagctg atctatgaga
     1561 tctatgagga gatatgcgga agccggctga cgaccaatat gggccgtatc ggcgggttcg
     1621 agcgcaactt cagcaatgcg gcgtgggaga agctcgagaa attcatggcc gagtatccgg
     1681 ccgtgctgaa agaattcgag aaccttttca cccgcaaccg catctttatg gagcggacca
     1741 tcggggcggg acccatcagc ggagaccgtg cacttaatta cgggtttacg ggccccaatc
     1801 tacgggccgc gggagtcgat tatgatgtcc gtgtacattc gccctatagc agctatgagg
     1861 acttcgactt cgagatcccg gtcggttcga cgggcgattg ctatgaccgg tttctcgtac
     1921 gcgaggggga gatgtggcag tcgatgcgga tcatcgagca ggcttacagg aagatacagg
     1981 atatgaaggg tgccgatgca gacgtctatc atgcggatgc gccggaatat tatctgccta
     2041 agaaagagga tgtatatacg aagatggagg ccctgatctg gcatttcaag atcgtgatgg
     2101 gagagacgca gataccgccg ggtgaggtgt atcactccgt ggaagggggc aatggggagc
     2161 tggggttcta tctgatcagc gatggggggc ggaatccgta cagattgcat ttccgccggc
     2221 cctgttttat ttattatcag gcttatagcg agctgacgca gggagcgatg ctgagcgatg
     2281 cgatcattgt gatgagctcg ttgaatctta ttgctggcga aatggatgca taataatatg
     2341 atacgatttt cagacgaaaa attgaagaag gtggacgaga tcgtcgcgcg ctacccggcg
     2401 gggaagcaga agagcgccgt tattccggtg ctgcatatgg cgcaggagga gttcggcgga
     2461 tggctgagcg tggatgcgat ggattatgtg gcggggttgc tgaagctgga gccgatagag
     2521 gtgtatgagg tggcgacgtt ttattccatg ctgaatacga aaccggtagg aaggttcgta
     2581 ttcgaagttt gtcagacggg tccctgtatg gtgcgtggtt gcgacgacat catcgggcat
     2641 atcaagaccc ggttgggcat cggggtcgga gagacgacgg cggatgggtt gttcacgctg
     2701 aagaccgtgg agtgccttgg ggcatgtggc tacgcaccga tgatgcaact agggaaatat
     2761 ttccgggagc atttgacgaa agagaaagtg gacgccatca ttaaagagtg cagggagaag
     2821 gcggcggtga ataattaacg accgtgaaaa gaaagaaatt caggatcatt actttcagcc
     2881 tgcttgctgc gggactgatc ccgatgatcg cgagcatggg tattgagaag gaatcgatga
     2941 aggaactgct gctgggagtg accgggcttt tgtatctggc tgctgctatt cagattgtaa
     3001 tatataaaag aaatgggtag aaaactttta ttggacaagg caaatgtgga aggcatccgc
     3061 ggttacgatg tgtatcgtcg tgagggcggt tatgccagcg tggaaaaagc gctgaagacg
     3121 atgactccgg accaggtgac ggaggaggtg aagaagagtg gtcttcgcgg tcgcggcggc
     3181 gccggttttc ccacgggaat gaagtggagt tttttggcaa agcctgaagg cgtaccgcgc
     3241 tatctcgttt gtaatgcgga cgaatcggag ccggggacgt tcaaggaccg gtacctgatg
     3301 gaattcctcc cgcacctgct gatcgagggg ctgatcgtct cgtcgtttgc cctgggcagc
     3361 aagagcacct atatctatat ccgcggcgaa tatgcctgga tacccgagat actcgaacag
     3421 gccatcgccg aggcgaaggc caacgggtgg ctggggaaga atattctggg caccggcttc
     3481 gattgcgaaa tttacgtcca cacgggcgcc ggggcgtata tctgcggtga agagaccgca
     3541 ctgatcgagt cgctggaggg caaacgcggg aacccacgta taaagccccc cttccccgcc
     3601 gtcaaaggcg tatgggattg tccgacggtg gtcaataacg tcgagacgct ggccgcggtg
     3661 gtgcccatca tcaataatgg cggtgaagcc tattcgaaga tcggcgtcgg caaatcgacg
     3721 ggcaccaagc tgatctcggc gtgcggcaat atcaataagc caggggtcta tgagatcgag
     3781 atgacgatgt cggtagaaga atttatctat tccgacgaat tttgcggcgg gatcccgaat
     3841 gggaagaggc tgaaggcctg tatcccgggt ggatcgtcgg tgcctatttt gccggcgaat
     3901 ttgctgttga agaccgtcaa gggcgagacg cgtctcatga actatgagag tcttagcgac
     3961 ggcggttttg ccaagggatc gatgatggga tcgggaggat tcatcgtgct ggatgaagac
     4021 cagtgcatcg tcaggaatac gtatacgctg gcgagatttt accggcatga gagctgcgga
     4081 cagtgttcgc cttgtcgcga ggggacggga tggatggaga agatcctccg gaacatcgat
     4141 accgggaagg ggaagaagag cgatatcgat ctgttgtggg atattcaaag gaagatagag
     4201 ggaaatacga tatgcccgct tggtgacgcg gcggcatggc cggtcgcggc ggcgataagg
     4261 catttcaggg atgagttcga gtggcatgtc gataatcctc aggagtgttt ggtgaggaat
     4321 tatgggctag cacactatgc ggatccgctg gaggcggcca cggcataaaa actttattta
     4381 tggctgacga aaagaaactt tttaaggtta cgattgacaa tatcaccatc gaggtggaac
     4441 cggggacgtc gatcttgcaa gcggcccgga ggatcggcgg ggacgtagct ccgcccgcca
     4501 tgtgttacta caccaagctg aagggtagcg gcgggaaatg tcgcacctgt ctggtggagg
     4561 tggccgccgg cagtacggcg gacccgagac cgatgcccaa gctgatcgcc agctgcagaa
     4621 cgaatgtgat ggatgggatg gtcgtcaaga atattacgag cgagaaagtg atcgatgcac
     4681 ggaatggggt cgtagagttc ctgttgatca atcaccccct ggattgccct atctgcgatc
     4741 aggccggcga gtgtcatctg caggacttaa gctacgaaca tgggaaagag gggacgcggt
     4801 atgagtttac acgcaggacg ttcgaaaagc atgacctggg tccttatatc cagctgcata
     4861 tgacacgctg tatattatgc taccggtgtg tgtttgtcgc cgaccagatc actgaacggc
     4921 gggagcatgg ggtcctggac cggggcgacc aatcggagat cgccaccttt attcagaaat
     4981 cgctggatca cgactttata gggaatgtca tcgacgtatg cccggtgggt gcgttgacgg
     5041 ataagacgtt ccgtttcaag aaccgggttt ggttcctgaa accggtggac gcgcaccgcg
     5101 attgcaatca cccgaagtgc aaagggaatg tcaccctatg gtaccggggt gaggaagtat
     5161 ttcgtgtcac ggcgcgcaag gatcagtggg gtgaggtgga ggactggatc tgtaatgagt
     5221 gccggttcga gaaaaagaaa acttcggact gggtgatcga aggaccgacc aaggtcagcc
     5281 ggcattcggt gatctcgcag ggacactatg tcggagttaa caggccgcag gaggtgttgt
     5341 atgatgtgct ggacgggcgg aagccgcggt tgctgatgga tatacattcg gtaagcgagg
     5401 tgaacagacc ggaaatagat ttgagcaaga tagaggggcc ggcgcattcg acggattttg
     5461 agccccatga gccgcgcggg accacaggcg tagaggcagg caatccctgg gatacaggca
     5521 aactggaaaa gtaaaataaa gcagaatgac cttactggct attgacatgg ttttattgct
     5581 ggagaaagcc atactgatcc tgatcatcat cagcgtttcg ctgctggtgg cgatgtatga
     5641 gacatgggcg gagaggaagg tggcggcctt tatgcaggac aggcggggcc ctaaccgggc
     5701 ggggcctgcg gggctgttcc agccgctggc cgacggattg aagctcttta tgaaagagga
     5761 gatcatcccg ctgagctcga accggtttct gttcgtattg ggtccgggtc tggcgatgct
     5821 gacggccatg atgacgagtg cggtcgtgcc ctggagcagt cattttacca tcgggggccg
     5881 tgaggtctcg ttgcagatag cagacgtgaa tatcggcatc
//
LOCUS       DASYSV010000085         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1299161, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000085 DASYSV010000000
VERSION     DASYSV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299161"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VGQ51_02610"
     CDS             <1..391
                     /locus_tag="VGQ51_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035478.1"
                     /translation="EQMKASDALVAQLQTLGKMIWNAGLPVLDDLHNFSYDWKVPDSV
                     AHNDRKLQRYRVAEYIDALRRLQPGVTMMIMHCSDAGEHFTHISDSGPVRRGDMLAML
                     DPAFRRALKDEHIILTTWRELMRRRSR"
     gene            complement(439..873)
                     /locus_tag="VGQ51_02615"
     CDS             complement(439..873)
                     /locus_tag="VGQ51_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035479.1"
                     /translation="MQDDVLMLPPTFKLYKDRAIAIGTFVGGPLVAGYLAAENFKQLG
                     QKSGAKAAWVISVLVTIIIFGGIFLIPDIDKVPRYIIPLSYTALAQVLVGKYQGAAIK
                     KHIDDGGQTYSAWRAVWIGLIGLAILVAIILCILYFAAPDLT"
     gene            952..>1821
                     /locus_tag="VGQ51_02620"
     CDS             952..>1821
                     /locus_tag="VGQ51_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9035480.1"
                     /translation="MVVQSLPRSFNPDNEITPPVYSDHFLFAEQLRQRYECAEYPAGI
                     GLLAAMEGRCDYFVNGAKQPIDGTRSFFINRGSRLAVKVAAKDSAPALLFFRTALPDL
                     VQHSLNYGNEVLLDEPFDNLPYDFSYLERTHLGPRLHETVASLIELGSSCSAFSSLSA
                     DLLIRHLFEDLLRANRDGYKLSQNIQAAKVSTRLEIFRRVSHARDWMEENYGINISLE
                     DIAAEAAMNSQHFLRMFKQVYLITPHQYLIERKLKKARQLLESTEMTINEICQAIGFE
                     SVFSFSILFKNRFG"
BASE COUNT          445 a          417 c          534 g          425 t
ORIGIN      
        1 ggaacaaatg aaggcgtccg acgcgctggt agcccagttg cagacgctcg gtaaaatgat
       61 ctggaacgcg ggactacctg tgttggacga tctgcacaat ttcagctatg actggaaggt
      121 gccggacagc gtcgcgcata atgaccgaaa attacaacgc tatcgggtag cagaatatat
      181 cgatgcgtta aggcgtttgc agccgggagt cacgatgatg atcatgcact gctccgatgc
      241 gggcgaacat tttacgcata tcagcgatag tgggccggtg cggcgtggag atatgcttgc
      301 gatgctggac ccggcgttca ggagggcgtt gaaggacgag catattattc tcaccacgtg
      361 gagggaattg atgagacgaa ggagccggtg aaggaatgga ggagggaatt gatgagacga
      421 aggagccggt aagtcagctc aggttaggtc gggggctgcg aaataaagaa tacaaagaat
      481 gatagcgacg aggatagcaa gaccgatgag cccgatccaa accgcccgcc aggcggaata
      541 ggtctggccg ccgtcatcga tatgtttttt gatggcggca ccctgatatt ttcccaccag
      601 gacttgcgcc agggccgtat aggataaggg gatgatgtat cggggcactt tatcgatgtc
      661 ggggatgagg aatatcccac cgaaaattat gatggtcacg aggaccgaaa tcacccaggc
      721 tgcctttgcg ccggactttt ggccgagctg tttaaagttt tcagcggcca gataacctgc
      781 gaccaatggg cctcctacaa aagttccgat ggcaatcgcc cggtctttgt agagcttgaa
      841 agttggtggt agcatcagta cgtcgtcttg catggctgtt ggattcaggg ttcgaatttc
      901 aagataagca aatccgggcg tatattggag tagatttgaa gataatatac catggtggtc
      961 caatcacttc cgaggtcatt caatcctgac aacgagatca cgccgcccgt ttattcagac
     1021 catttccttt tcgcggagca gctgcggcag cgctatgaat gtgccgaata cccggcgggg
     1081 atcgggcttc tggcagccat ggaggggaga tgcgattatt ttgtgaacgg ggctaagcaa
     1141 cccatcgacg ggacgagatc attctttatc aacaggggca gcaggctggc cgtcaaagtt
     1201 gccgcaaagg attccgcgcc tgcactgttg ttctttcgta ccgctctccc cgatctggtg
     1261 cagcatagtc tcaactatgg gaatgaggtg ttgctggatg aacccttcga caatctgcct
     1321 tatgactttt cttacctgga aaggacgcat ctcggcccgc gtttgcatga gaccgttgca
     1381 tcgctgatcg aattggggag tagctgctcc gccttttcct cgctgagcgc ggacctcctg
     1441 atcaggcatc tgttcgaaga cctcctgagg gctaacaggg atgggtacaa actttcgcaa
     1501 aatattcagg ctgccaaggt gtcgacccgg ctggagatct tccggcgggt gtcgcatgcc
     1561 agggattgga tggaggaaaa ctacggtata aatatcagtc ttgaagatat tgccgccgag
     1621 gcggccatga acagccagca ttttctgaga atgttcaagc aggtgtacct tattactccg
     1681 catcaatacc tgatagagcg caagctgaaa aaggcaaggc aattgttgga gtctaccgaa
     1741 atgacgatca atgagatatg ccaggcgatc gggttcgaga gcgtgttttc tttcagcatt
     1801 cttttcaaga atcgttttgg g
//
LOCUS       DASYSV010000086         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1299220, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000086 DASYSV010000000
VERSION     DASYSV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299220"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VGQ51_02625"
     CDS             <1..997
                     /locus_tag="VGQ51_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9035481.1"
                     /translation="AAIEKATAQFKLAGSAYDNALFQQRYAGVLTYLGIIYLQKLLKA
                     EQENIDRSQVSLQQSLVLAQQGLRPGLDTVQFQSTLAQARMQWLNTESIYQAQVLELC
                     RLTGLPGTPDAYVFSDTLMAARLPAAPDTSGGYVQNPAYRYYEAQVSLGAANLMQIQR
                     GWRPTLNLWGNVYARGSGIEPNGVIDNAQGWALTRSNLAAGIQLSFPILQFSTVNIQK
                     KQYRSLLKSDQNQLSQMGINLQRQIETAESNYRQNLRIAEEAPIQSRAARLSYHGLEL
                     SYKSGLVDFTQLTQGQYQLLNAETMEANAYLQVWKSLLDIGVAKGNLNLFTDELK"
     gene            1010..>2616
                     /locus_tag="VGQ51_02630"
     CDS             1010..>2616
                     /locus_tag="VGQ51_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9035482.1"
                     /translation="MNKLIISALRHPITILVTVISILFFSWLAIRNTKVDIFPRLGLP
                     TVYVAQPYGGLSPDQMEGFVTSYYEYHFLYITGVKYVDSKSIQGASLIKIEFNEGTDM
                     AQAMAEVVGYVNRSRAFMPPGTVPPFIMRFDAGSVPVGQLVFSSETRSLTEINDLALF
                     RVRPMFSTLPGVSAPPPFGGNQKTVVVTLDPEKIRAYNLSPDQVVQALVKFNGITPAG
                     NVSIGDTTYLTPQNSVIEDVQDLQKVPLQLGSGPTVYIRDIATVSLGSDITSCYALIN
                     GKRSVYIPVTKRADASTWDVVQRVKAALPDMQAAIPADIKVSYEFDQSGYVINSLKTV
                     LFEGGLGALLTGLVVLLFLADMRSALIVIMTIPLALLSAATALYLCGQSINIMTLGGL
                     ALSVGILVDETTVTIENIHRHQELGKDRGRAIADACLEIAGPKLLILLSILAVFVPSL
                     FMNGVPRAMFLPLSLSVGFAMIASFLLSQTFVPVVSNWLLKHRIDEGRKSSAFQRMKD
                     RYVRGGRRFHRSYALFTMLFLLVAVVLV"
BASE COUNT          589 a          732 c          678 g          617 t
ORIGIN      
        1 cgccgccatc gaaaaggcta cagcgcaatt caaactcgcc ggcagcgcct atgataatgc
       61 cctttttcaa caacgttatg cgggtgtcct tacttacctg ggcatcatct atcttcagaa
      121 gcttctgaaa gctgaacagg agaatatcga tcgatcgcag gttagtctgc agcaatccct
      181 ggtactggcc caacaagggc ttcgtcccgg gttggatact gtgcagttcc aatcgacgct
      241 ggcacaggcc agaatgcaat ggctcaacac ggaaagcatc tatcaggcac aggtactcga
      301 gctgtgccgg ctgaccggtc ttcccggcac accggatgcc tatgtattta gcgatacgct
      361 gatggcggcc cggcttcctg cggctcctga taccagcggg ggttatgttc aaaatcccgc
      421 ttatcgttat tacgaggcac aggtatctct tggtgcagcc aacctgatgc aaatacagcg
      481 tggctggcgc ccgaccctca atctgtgggg gaatgtctat gcgcgaggct cgggcatcga
      541 accgaatggg gtcatcgata atgcgcaggg ctgggccctt acacgcagca acctggccgc
      601 aggtattcaa ttaagcttcc cgatcctgca attttccacg gtaaatattc aaaagaagca
      661 gtaccgatcc ctgctgaagt ccgatcagaa ccagctatcc caaatgggca tcaaccttca
      721 acggcaaatc gaaacggcgg agtccaatta ccggcagaac ctgcgcatag cggaggaagc
      781 gcccatccag tcgcgggcag cccggctttc ctaccacggg ctggagctta gctacaagag
      841 cggcctggtc gatttcaccc aactcacgca agggcagtat caactcctga acgcggaaac
      901 gatggaagcg aacgcttacc tgcaggtatg gaagtcgtta ctggatatcg gggtagcaaa
      961 aggcaatctg aatctattta cagatgaatt gaaataaccg tattggacca tgaataaact
     1021 catcatttcg gcgctgcgac atcccatcac gatcctggta acggtgatct ccatcctgtt
     1081 cttttcctgg ttggccatcc gcaataccaa agtagatatt tttccccggc ttggtcttcc
     1141 gaccgtctat gtcgcgcagc cttacggcgg cctgtcaccg gaccagatgg aagggttcgt
     1201 cacctcctac tacgaatacc attttcttta tataaccggg gtgaaatatg tcgattcgaa
     1261 gagtatccag ggtgcttcgc tcatcaagat cgagttcaat gaaggaacgg atatggcgca
     1321 ggctatggcg gaagtcgtag gttatgtcaa ccgctccagg gccttcatgc ctccgggaac
     1381 ggtccccccc ttcatcatgc gttttgacgc cggaagcgta cccgtcggtc aattggtgtt
     1441 cagctccgag acgcgctcgc tcaccgagat caatgacctc gctcttttcc gggtccgccc
     1501 catgttctct acgcttcccg gcgtctctgc gcccccaccc ttcggcggaa atcagaagac
     1561 cgtggtggtg accctcgacc cggaaaagat acgcgcctac aatctttctc ccgatcaggt
     1621 cgtacaggca ctggtcaaat ttaacggcat tactcccgcc ggtaacgtca gcatcgggga
     1681 cactacctat ctcacacccc aaaacagcgt catagaagat gttcaggatt tgcaaaaagt
     1741 acctctgcag ctgggtagcg gccctacggt ttatatcaga gacatcgcaa ccgtaagtct
     1801 cgggtcggat attacaagct gctatgcgct tatcaacgga aagcgttcgg tttatatacc
     1861 tgtgaccaaa cgtgcggacg cttcgacctg ggatgtcgta caacgggtaa aagccgcgct
     1921 gccggacatg caggccgcga tccctgccga tataaaagtc agctatgagt tcgatcaatc
     1981 gggctatgtg atcaattcgc tcaaaacggt attgttcgaa ggaggcctcg gcgcgctgtt
     2041 gaccggtctg gtcgtactgc tttttctggc agatatgcga agcgcactca tcgtaatcat
     2101 gaccatcccg ctagccttgt tgtccgcggc cacggccctt tatctctgcg gacagtccat
     2161 caacatcatg accctcggtg gtctggcgtt atcggtcggt atactggtgg atgagaccac
     2221 cgtcaccatc gagaatatcc accgtcacca ggaattgggt aaggacagag gtcgcgccat
     2281 cgcagacgcc tgtctggaga tagccggacc caaattgctg atcctgctga gtattcttgc
     2341 cgtattcgtg ccatcgctgt tcatgaatgg cgtgcccagg gctatgttcc tgccgctgtc
     2401 gttgtcggtg gggtttgcca tgatcgcttc tttcctgttg tcgcagacgt ttgtacccgt
     2461 cgtttcgaac tggctgctaa aacaccggat tgacgagggc cggaaatcca gtgcattcca
     2521 acggatgaag gaccgatatg tccgcggcgg acgacggttt catagaagct atgcgctgtt
     2581 tactatgctg ttccttctcg ttgccgtcgt tttggt
//
LOCUS       DASYSV010000087         8861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_18357, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000087 DASYSV010000000
VERSION     DASYSV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18357"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VGQ51_02635"
     CDS             <1..425
                     /locus_tag="VGQ51_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV9035483.1"
                     /translation="VVIESGFADKQFFYGKGAGSFPTASAVLSDLSALRYEYKYEYKK
                     LYHHTPHQLTDDFFVRVYLSFDDLKYIPKDRFASIEEWHADNDRKYLVGVIHFREIQG
                     QNWWRRNGTSLILTPDPIIEDMEVRRLKKKSLELAGLI"
     gene            complement(402..2168)
                     /locus_tag="VGQ51_02640"
     CDS             complement(402..2168)
                     /locus_tag="VGQ51_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9035484.1"
                     /translation="MANPYLSLLRTSWHYSRNERKRYVLVYSLFVCASLVNACNPLLL
                     GWFIGRLQKDMTHVWHFTLLYVIGYIGLKFFQWCFHGPARIMERMLAFNLSRNFLQEK
                     YHQVLHLPVKWQQDHHSGAIINRVRKAYESLRDFFDRGFIFLYTLLKFVISVVAILYF
                     SPLFGSIAVVMGIGLLFVIRTFNKPFLKAVDEVNEKEHIVSATLFDSLSNIMTVITLR
                     LERSMETGLLARVRDIFRPFRRSAVVNEWKWFCAEMIIVLIYSMITMGYIWQHWTPGR
                     LFEVGGLVALLGFVQQFTSVFTDVAFQYTNIMLYDSYVQTASVISEAYAERHRADRSD
                     GLSDGWDTLRIDRLHFSYHAEYDSHRAQSLHDLNLTIRRGQRIALIGESGSGKTTLLS
                     LLRGLYNPEPGTGILLDGRETSLEQLNESVTLFPQEPEIFENTLAYNITLGLPFPDEE
                     VLRACDAAHFTDVIRQLPQGLETDIREKGVNLSGGQKQRLALARGILAARDSAIVLLD
                     EPTSSVDPRTEALIYDGLFSAFADKAVISSVHRLHLLNHFDYIYILHQGRIIGEGTFD
                     QLLANDPAFGEMWRHSNKSRQL"
     gene            complement(2161..3375)
                     /locus_tag="VGQ51_02645"
     CDS             complement(2161..3375)
                     /locus_tag="VGQ51_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9035485.1"
                     /translation="MLKEISRISLENEMDIVVAHKRMLGVAQYFSLLLSTQATIATAV
                     AEVTRAVIERTDDGWLTIGIDDEGGRFTLVAIISFSPETDIRASDEGLRYAQMLVPDF
                     RIDREKEVASIYLGIGIPRSLKVSKARIGEATEYFQGIQPTTPYERLKLRNSFLNQQA
                     LEREAELRHSRLLDQKKNEFISIASHELKTPLTSLIAFTKLALSTDQSEASPRVRRYL
                     EKIDMQTYKLHQMIQQLLDIARIESGRMDYNMQPREWNGYMGEMMLTLEQMVPTHRLS
                     WQPCEGPVIVTMDVLRIEQVLTNLVNNAAKYSDSDTPINIVCSTDEEALTVCVRDQGI
                     GISRQNITRIFEKYFRDEAVTNKYSGFGMGLYITSGIVREHRGTIWAENNEQQAGSSF
                     YFTLPLKPDGHG"
     gene            complement(3379..4389)
                     /locus_tag="VGQ51_02650"
     CDS             complement(3379..4389)
                     /locus_tag="VGQ51_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEV9035486.1"
                     /translation="MGNRHYKSFRADDRSFFSLIKKEIHQQVASAGFDEIRIGKIDII
                     IAEITSNLSKYAVGGQLFAGIGSDAYGEYFDVIGLDGGPGIPDLSRVLSDGYSSTGSL
                     GHGLGSIRRLSDQFDLFSMKDWGTILISRVYKNDLPRTRKKKIDCQGLNVPKAGESVS
                     GDGFYIRDTPDTWSILVADGLGHGAGAHAAVEKAGEVFAECPEESPTEIIRYIHHRIR
                     KTRGIVGVIIVLDKKTRRWKNAGVGNISLKWTGHNTSRTYISYNGIIGFNIPGSMNDQ
                     DLSQDEFHEFIACSDGIRSRWDLARFPGIGAHQGMIIASAIFKEFARGTDDSSVIVCK
                     SV"
     gene            complement(4382..4795)
                     /locus_tag="VGQ51_02655"
     CDS             complement(4382..4795)
                     /locus_tag="VGQ51_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEV9035487.1"
                     /translation="MILSLTKDKVVIEKEQDVILFRNRVKEFAQKIKMSLVNQTKLIT
                     AASELVRNMLKYANGGNITIEVVSRGRDNGIRLVFSDQGPGISDINLAMKDGWSTTKS
                     LGLGLPGAKRLVNDFNIISELNKGTVITIIKWANG"
     gene            complement(4776..5186)
                     /locus_tag="VGQ51_02660"
     CDS             complement(4776..5186)
                     /locus_tag="VGQ51_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV9035488.1"
                     /translation="MDKIPILRMGPFLLVTIQVDLYDRLALNLEADLVQAVSKTGARG
                     VLIDISAVSIVDSFMGRIIGNIASMSRILDAETVVVGMQPAVAITLVELGLPLKGVHT
                     ALDVERGMDLLRSKIDYGVDGDQESTEENDIVAD"
     gene            complement(5200..6063)
                     /locus_tag="VGQ51_02665"
     CDS             complement(5200..6063)
                     /locus_tag="VGQ51_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV9035489.1"
                     /translation="MAIDITKQLKTKRKALFERWMQNQLTSDGLREDLLSNEELRSTS
                     EELIDVFLQNLTPENLDDPTSTSFEPVLDILSGISISRARQGFSPRETGMFVISLKEA
                     LQAQLQEEIKDNPKQLYEESLKLGRIMDHFAINSFETYIKGREEVILRQTDEMAEIST
                     PVIRVWDGILALPIIGTLDSSRTQVIMEALLNEIVTSGSSITILDISGVPTVDSLVAQ
                     HLIKTVSATRLMGAECIISGIRPEIAQTIVHLGIDLTGIITKSSLASALQFAFQALNL
                     QVKKNHLQKQA"
     gene            6171..6539
                     /locus_tag="VGQ51_02670"
     CDS             6171..6539
                     /locus_tag="VGQ51_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9035490.1"
                     /translation="MKKKILIFDDDADILEVCTILLEGNGFEAYTQNNCEDILQKLDH
                     VLPDVVLMDNKIPPNGGIPASRMIRASADYRTVPIVFFSANQDVARLAAEAGADTYIE
                     KPFDLDNLVRVLQTASSPKV"
     gene            complement(6682..8745)
                     /locus_tag="VGQ51_02675"
     CDS             complement(6682..8745)
                     /locus_tag="VGQ51_02675"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEV9035491.1"
                     /translation="MPFSRLPVCIAVLLISSSCFAQPITLRSPDGDLRFAFGRGDKLP
                     VYSVWYREKPLISASPLDLFFLETGDKQPSSPFGRGWRAGPGVYREGKDDYTLPVGRN
                     SAVHDRYNEYRIPLEETGGRRRRLSLVVRLYNDGVAFRWSFPQQPNRRSFRLTAENTG
                     FRLEGNPILLASFLPGFTTSHENRYTRLPFSEVRPDTLMDMPVFLQWPGESSKTGIRD
                     SLYMVITEAQLTDYAGMYLAKHDGMLRSELSPLPIGPAVVETSPVAATAHNALRVKAT
                     LPHRSPWRVIFIGPRPGAFLESNLLTDLNDPCAIKDLSWLHPGKTDFHWWNGDILPDS
                     TISPGINFETNQYYIDFCARNGIDYHTVIGFGGIAWYTNDGVSYDAGPHSDPSKPIPG
                     LDMKQICDYAASKGVGVRVWVHWKALWPHIDSAFTRMQRWGIKGMMVDFMNRDDQEMV
                     NIQTTILRKAAQHHLHIQFHGAYKPTGLIRTWPNELTREGTLNYENDKWNDPITPDDD
                     LNVVFTRLMAGATDYHLGGFRAVTPQGYKPQFTRPLVLGTRCHMLAMYIVLESYLPMV
                     CDYPAAYEGQKGFDFLKSVPSTWDETRVPVAIPGEWVAIARRKGDDWYVGAINDHAAR
                     EACIPLNFLPPGRYVATIYADAPDADTDPNHLVITTREVGPADCLHVALAGGGGQAIR
                     IAPAK"
BASE COUNT         1967 a         2324 c         2510 g         2060 t
ORIGIN      
        1 gcgtagtgat cgaaagcggt tttgcggaca agcaattctt ctatggcaag ggcgcgggat
       61 cctttcctac cgccagcgcg gtgctaagcg atctttcggc gctcaggtat gaatataaat
      121 atgagtataa gaaactctat caccatacgc cgcatcagct gaccgacgat ttcttcgtcc
      181 gcgtctatct cagcttcgac gatctgaaat atatcccgaa ggaccgtttt gcatcgatag
      241 aggaatggca tgccgataat gaccggaaat acctggtagg agtgatccat ttccgcgaga
      301 tacagggtca gaactggtgg aggcggaatg gtacgtcgct gatcctgaca cccgatccca
      361 tcatcgagga catggaagtg cggcggctga agaaaaagag tttagagctg gcgggactta
      421 tttgagtgtc tccacatctc gccgaaggcg ggatcattgg caagaagctg gtcgaaggtg
      481 ccctctccga tgatgcggcc ctggtgaagg atatagatat agtcgaagtg gttgagcaaa
      541 tgcaaccggt gtacggacga gatcacggct ttgtcggcaa aagccgagaa caatccgtca
      601 tagataagcg cctccgtgcg cgggtccacg ctgctggtag gttcatcgag caggacgatg
      661 gcgctatcgc gtgccgcaag gataccccgg gccagggcta accgctgttt ctgcccgccc
      721 gaaagattca cccccttttc acggatatcc gtttccagac cttgcggcag ctggcggatc
      781 acgtcggtaa aatgagccgc gtcgcaggcg cggagcacct cttcatccgg gaaaggcagt
      841 cccagcgtga tgttataagc caacgtattt tcgaagatct cgggttcctg cgggaaaagc
      901 gtaacggatt cgttcagctg ttcgagcgag gtctcgcggc cgtcgagcag gatgccggtg
      961 ccgggttcgg ggttgtagag accgcggagc agcgaaagca gcgtggtctt cccgctgccg
     1021 ctctcgccga tgagtgcgat gcgttgcccg cgccggatgg tgaggttcag gtcgtgcagg
     1081 ctttgggcgc ggtggctatc gtattccgca tgataggaga aatggagacg gtcgatccgg
     1141 agggtatccc agccatcgga tagtccgtcg gacctgtcgg cgcggtgtcg ttccgcatag
     1201 gcttcactga tgaccgaggc cgtctgcaca tagctatcgt acaacatgat attggtatac
     1261 tgaaaggcga cgtccgtaaa cacgctggtg aactgttgga cgaagccgag cagcgcgaca
     1321 agaccgccta cctcgaacag ccggccgggg gtccagtgct gccagatata ccccatcgtg
     1381 atcatactgt aaataaggac gatgatcatt tccgcgcaga accatttcca ttcattgacc
     1441 accgcgctgc ggcggaaagg acggaagatg tcgcgcaccc tcgcaagcag gccggtctcc
     1501 atgcttcttt ccagtctcag cgtgatcacg gtcatgatat tggacagact gtcgaagagg
     1561 gtggcggaga cgatatgttc cttctcattg acctcatcca cggccttcag aaaaggtttg
     1621 ttgaaggtgc ggatcacgaa gaggaggccg atgcccatga ccacggcaat actgccgaac
     1681 aacggcgaaa aatacaggat ggcgaccacc gagatcacaa atttgagaag ggtatataaa
     1741 aaaataaagc cgcgatcgaa gaaatcccgc agcgattcat atgccttgcg aacgcggttt
     1801 atgatcgcgc cgctgtgatg gtcctgttgc cattttaccg gcaggtgcag cacctggtgg
     1861 tatttttcct gtaaaaaatt ccgggagagg ttgaaggcga gcatccgctc catgatccgc
     1921 gcgggtccat ggaaacacca ttggaagaat ttgagaccga tatagcctat gacataaagc
     1981 agcgtaaagt gccagacatg cgtcatgtcc ttctgtagcc ggccaatgaa ccagcccagc
     2041 agcagcgggt tgcaggcgtt gaccaggctg gcgcatacga acagactata tacgaggaca
     2101 tatctcttcc tttcattacg ggaataatgc catgaagttc tgagcagtga aagataagga
     2161 ttagccatgg ccatccggct tgagcggcaa ggtaaaataa aaggacgagc cggcctgttg
     2221 ttcattgttt tcggcccaga tggtaccccg atgttcacga acgatcccgg aggtaatgta
     2281 cagtcccatg ccgaagccgc tgtatttatt ggtcaccgcc tcatcccgga aatatttttc
     2341 gaagatgcgg gtgatgtttt gccgggagat gccgataccc tggtcgcgga cgcatacggt
     2401 gagggcttct tcgtcggttg agcaaacgat attaataggc gtgtcggaat ccgaatattt
     2461 ggcggcattg ttcacgaggt tggtgagcac ctgttcgatc cgcagtacat ccatggtgac
     2521 tatgacgggg ccttcgcagg gctgccagct gagccggtgg gtaggcacca tttgttcgag
     2581 cgtgagcatc atctcgccca tatagccgtt ccattcccgg ggctgcatgt tataatccat
     2641 gcggcccgac tcgatcctgg cgatgtccag caattgctgg atcatctggt gcagcttgta
     2701 ggtctgcata tcgatctttt cgagatagcg ccgtacccgg ggcgaagcct cggattgatc
     2761 cgtgctgagg gccagtttcg taaaggcgat cagcgaggtg agtggtgtct tcagctcgtg
     2821 gctggcgatg gagatgaact cgttcttttt ctgatcgagc aggcggctat ggcgcagctc
     2881 ggcttctctt tcgagggcct gctggttgag gaaggagtta cgcagtttga gccgttcgta
     2941 cggcgtggtg ggttgtatcc cctggaagta ttcagtcgcc tcgccgatac gcgctttcga
     3001 gaccttcagc gatcggggga tcccgatgcc caggtagatg gaggcaacct ccttttcgcg
     3061 atcgatgcgg aagtcgggga ccagcatctg ggcgtatcgg agaccttcat cggaagcccg
     3121 gatatcggtc tcgggcgaaa agctgataat ggcgacgagc gtgaatctgc cgccctcatc
     3181 gtcgatgccg atagtcagcc agccgtcgtc cgtcctctcg atcaccgccc tggtgacttc
     3241 cgcgaccgct gtggcgatgg tagcctgcgt ggacagaagc agagaaaaat attgcgcaac
     3301 gccgagcatg cgtttgtgcg ctaccacgat atccatttcg ttctccagcg aaatccggct
     3361 gatctcttta agcataggtt aaaccgattt acagacgatt accgatgaat catccgtccc
     3421 acgggcaaat tccttgaaga ttgcagaggc aataatcatt ccctggtgcg cgccgatgcc
     3481 tggaaagcgc gccagatccc accgggagcg aattccgtcg gaacaggcga tgaattcatg
     3541 gaactcgtcc tgggacagat cctggtcatt catggagccg gggatattga atccgatgat
     3601 cccgttatag gatatatagg tacggcttgt attgtgcccc gtccacttta gcgatatgtt
     3661 ccccacacct gcatttttcc aacggcgggt ctttttgtcg agtacgatga tgacaccaac
     3721 gatgccgcgg gtcttgcgta tccggtgatg aatataccga atgatctccg tggggctctc
     3781 ttcggggcat tcggcaaaga cttctcccgc cttctccact gccgcgtgtg cacccgctcc
     3841 gtgacccagc ccatcggcca ccaggatact ccaggtgtcg ggtgtatcgc ggatataaaa
     3901 accgtcgccg ctgaccgatt cgcccgcttt tggaacattc agcccctggc agtcgatctt
     3961 tttcttccgt gtccggggca gatcattctt ataaacgcgg gaaatcagga tcgtccccca
     4021 atccttcatc gaaaaaagat cgaattgatc ggagagccgt cgtatgctgc ccaacccgtg
     4081 tccgaggctg cccgtagagg aatagccatc ggacaagacc cggctgaggt cgggaatacc
     4141 cggtccccca tccagaccga tcacatcgaa atattcaccg taggcatcag aaccgatacc
     4201 ggcaaacagt tggccgccca ccgcgtattt gctgagattg gacgtgatct ctgcgataat
     4261 gatgtctatt ttcccgatac gtatctcgtc gaatcccgcg cttgccactt gctgatggat
     4321 ctcctttttt atcaacgaaa aaaaacttcg gtcatcggca cggaagcttt tgtaatgcct
     4381 gttacccatt cgcccacttt atgattgtta tgaccgtgcc tttgttgagc tcgcttatga
     4441 tattgaagtc gttgaccagc cgtttggcac cgggaagacc cagacccagg cttttcgtag
     4501 tggaccaccc gtctttcatg gccaggttga tatcgctgat gcccggtccc tggtcactga
     4561 aaacgagtcg gataccgtta tcccttcctc gtgacaccac ctcgatcgtg atgttcccgc
     4621 cgttggcata cttgagcata ttccgtacga gctcgctggc ggcggtgatc aatttggtct
     4681 ggttgacgag gctcatcttt attttttggg caaattcttt cacgcgattg cggaacagga
     4741 tcacgtcctg ttctttttca attacgacct tatctttagt cagcgacaat atcattttcc
     4801 tcggttgatt cttggtcccc gtctacgcca taatcgatct tcgagcgaag caggtccatt
     4861 cctctttcta cgtcgagggc ggtatgtaca cccttcaagg gcagacccag ctcgaccagc
     4921 gtgatggcta cggcgggctg catgccgacg accacggttt ccgcatcgag tatcctggac
     4981 atgctggcga tgttcccgat aatgcggccc atgaaggaat cgacgatact gacagcggag
     5041 atatcgatca gcacgcctct tgcgccggtt ttgctgaccg cctgtacgag gtcggcctcc
     5101 agattcagcg cgagacggtc atacaggtcc acctgtatcg tcacgagtaa gaaaggtccc
     5161 attcgcagga ttggaatctt atccatgtct tagaaagatt taggcttgct tttgtagatg
     5221 attctttttg acctgcaaat tcagcgcctg gaaagcgaac tgcaatgcgc tggcgagcga
     5281 agatttggtg atgatgcctg tcagatcgat acccaggtgt acgatagttt gtgcgatctc
     5341 gggacggata ccactgatga tgcattcggc gcccatcagc cgtgtggcgc tcaccgtctt
     5401 gatgaggtgc tgggcaacca gcgagtcgac tgtcgggacg ccggagatat ccaggatggt
     5461 gatgctgctt ccggaagtca cgatctcgtt cagcagcgct tccatgatca cctgggtgcg
     5521 gctactgtcg agggtaccga tgatgggcag cgcgaggata ccatcccata cgcggatgac
     5581 cggggtagat atctcggcca tctcatcggt ctgacgcagg atcacctctt cgcggccctt
     5641 aatataggtc tcaaagctgt tgatggcgaa atggtccatg atccggccga gcttgagact
     5701 ttcttcatac agctgtttgg gattgtcttt aatctcttcc tgcagctggg cctggagcgc
     5761 ctctttcagg ctgatgacga acatcccggt ctcgcgggga ctgaaaccct gtctggcccg
     5821 ggaaatggaa atccccgaaa ggatgtcgag caccggttcg aaagaggtag aagtgggatc
     5881 gtccaggttc tcaggggtca gattctgtag gaagacgtcg atcagctctt cggaggtgga
     5941 tcgaagctct tcattgctga gcaggtcttc ccggaggccg tcgctggtca actggttttg
     6001 catccagcgc tcgaatagcg cttttctttt ggtttttaat tgtttggtaa tgtctatggc
     6061 catggaattc gagtttaaaa gaagtaaatt aaggcatcgg aaaataaaag tagcggataa
     6121 ttgaattaaa ttcgcggacg gaacttcaat aactaaacca tactgaccgc atgaaaaaga
     6181 aaatcttgat cttcgacgac gatgcggaca tcctcgaggt atgtactatc ttgttggagg
     6241 gcaatgggtt cgaggcatat acgcaaaaca actgcgaaga tattttgcaa aaactggacc
     6301 acgtgcttcc agatgttgtt ttgatggaca acaaaatccc gcccaacggc ggaatacccg
     6361 catcacgtat gatcagggca agtgcggact atcgaacagt tccgatcgtg tttttcagcg
     6421 ccaatcagga tgtcgcccga cttgccgccg aggccggtgc ggatacgtat atcgaaaagc
     6481 ctttcgacct cgacaacctg gtccgcgtac tgcaaaccgc cagcagtcct aaggtatagc
     6541 gccgcggcca tttcgggtat gtttcccgcg cggccatttc gggtatgttt cccgcgctgc
     6601 catttcgggt atgcttcccg cgccgaccgt tccacgcgca ttttcacttg tacattactg
     6661 ttagttcgac agccgctgca gctattttgc aggcgcgatc ctgatggcct gtccgcctcc
     6721 acccgccagc gccacatgca ggcaatccgc cggtcccacc tcgcgtgtcg taatgacgag
     6781 atgattggga tccgtatcgg catccggcgc atcggcatag atggtcgcca cataacgacc
     6841 gggcggaaga aaattcaacg gaatacacgc ttcgcgcgcc gcatgatcgt tgatcgcgcc
     6901 gacataccaa tcgtcaccct tgcgtcttgc gatggccacc cattctccgg ggatggctac
     6961 gggaacgcgg gtctcgtccc aggtggatgg cacacttttc agaaaatcga aacccttttg
     7021 tccttcatag gccgccgggt agtcgcagac catcggaaga tagctttcca ggacgatata
     7081 catcgccagc atatgacaac gggtgccgag tacgagcgga cgcgtgaatt gcggcttata
     7141 cccctggggt gtcaccgcgc ggaatccgcc gagatggtag tcggtagctc ctgccatcag
     7201 ccgcgtgaac actacgttga gatcgtcgtc gggggtgatg ggatcgttcc atttatcgtt
     7261 ctcgtaattc agcgttccct cgcgggtcag ctcgttgggc catgtgcgga tgagacccgt
     7321 cggtttatag gcgccgtgga attggatatg caaatggtgt tgcgcggcct tgcgcaggat
     7381 cgtcgtttgg atattgacca tctcctggtc gtcgcggttc atgaaatcga ccatcatgcc
     7441 tttgatgccc cagcgttgca tgcgggtgaa ggccgagtcg atgtgtggcc acagcgcctt
     7501 ccaatggacc catacgcgga cccccactcc cttcgaggcg gcatagtcgc agatctgttt
     7561 catatccaat cccggaatgg gtttggacgg atcggaatgc ggaccggcat catagctgac
     7621 tccgtcgttg gtataccacg cgatgccgcc gaagccgatc accgtatgat agtcgatgcc
     7681 gttacgggcg caaaaatcga tatagtattg attcgtctcg aaattgatac ccggcgaaat
     7741 cgtggaatcc gggaggatat cgccgttcca ccaatggaaa tccgttttcc cgggatgcag
     7801 ccaggaaagg tctttgatgg cgcaggggtc attcaggtcg gtgagcaaat tggactcgag
     7861 aaaggcgccg ggacgcggtc cgataaagat cacgcgccag ggactacggt gcggcaaagt
     7921 cgcttttacc cggagggcat tatgggcggt ggcggcgacg ggagaggtct cgacgaccgc
     7981 gggtccgatc ggcagggggg ataattcgct tctcagcata ccgtcgtgtt tggcgagata
     8041 catcccggca tagtccgtga gctgcgcctc ggtgatgacc atatagaggg agtcccggat
     8101 accggttttc gagctttccc cgggccactg gagaaaaacg ggcatatcca tcagggtatc
     8161 cggtctcacc tcgctaaagg gtagcctggt atagcgattc tcgtgagagg tggtgaaacc
     8221 cgggagaaaa gacgccagca ggatcgggtt cccctcgagg cgaaaaccgg tattttccgc
     8281 tgtaagccgg aaggaccttc ggttgggttg ttgggggaag gaccacctga aagccactcc
     8341 atcattatag agacggacca ccagggaaag ccttcgccgg cgtccgcccg tttcttccag
     8401 cgggatgcgg tattcgttgt aacggtcgtg gaccgcgctg ttccggccca ccggcagcgt
     8461 ataatcgtcc ttcccttccc ggtagacgcc cgggcctgcg cgccaccccc ggccgaatgg
     8521 agaagaaggc tgtttatccc ccgtctcgag gaaaaaaagg tccagcggag aggctgaaat
     8581 cagcggtttt tccctgtacc agacgctgta gacggggagt ttgtcgcccc gtccgaaggc
     8641 aaaccggagg tctccatcgg ggcttcggag ggtgatcggt tgtgcaaaac aggaggagga
     8701 gatcagcagg acggcgatac atacggggag acgggagaag ggcatgtagc tgttttttgc
     8761 aaattttaaa aaattatact tacaaattat gcatacagaa aaattaaaat ctgtacattt
     8821 atttcattaa cccattaaaa tgaaataatc atggcaaaag c
//
LOCUS       DASYSV010000088         8772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2067525, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000088 DASYSV010000000
VERSION     DASYSV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067525"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..944)
                     /locus_tag="VGQ51_02680"
     CDS             complement(21..944)
                     /locus_tag="VGQ51_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9035492.1"
                     /translation="MRSVRHFPILLPCVALSFIGCVPSGKFHDMEKQAHLSDSLYTQS
                     MRTLKGCQDDNIRLGKEKSTLQEHNKEMSQQVTAANEVNGQLRKQMEQLSAVSSAQAE
                     SIKRSLDNMGANDSYMIALHSAITYRDSVNLALLFILKQTIGGYGEQNVSIKLEKGAV
                     HVDLSDSLLFGSDSAGYTVSEKATQVLGHLARALTNAPDVEFTVEGQVDSLNAPADSV
                     HDNWDLALRRASAVVRILQNDQVSPTRMTAAGTTSLPVEHRTRILFTPKTELLSQLLE
                     HGPGGQSAAPPPPAPAGQSAAPPAAPAANSY"
     gene            complement(983..2062)
                     /locus_tag="VGQ51_02685"
     CDS             complement(983..2062)
                     /locus_tag="VGQ51_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HEV9035493.1"
                     /translation="MATPRRMLKWSNYVPFLSWIVYGLHYWIHSGIWLIPMVAGLIGS
                     VLAAVHHAEVIAHRVGQPFGVLVLAIAVTVIEVALIVSLMFAGGDEGLARDTVFAAVM
                     IILNGMMGLCLLIGGLKYRVQKFELEGVRSTLTVLVSISVLTLILPNYTSSASGPEYS
                     NTQLLFVGIVTLVLYATFVIVQNIRNRDFFLSESEKKEEEAAAEHPPTRRETTVSFLL
                     LLVCLLAVVIIAKALSPDLERLVDRMNAPHSLVGVIIAMIVLLPESVAAVRAARHDHL
                     QKALNLSLGSAVASIGLTIPTVSAVSIITGMPIALGIDIKSTLLFFLTLLVVAISLST
                     GRTTILQGIVLLVLFAVYLFMIIFP"
     gene            2113..3546
                     /locus_tag="VGQ51_02690"
     CDS             2113..3546
                     /locus_tag="VGQ51_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEV9035494.1"
                     /translation="MKPVWTIFLVLVLSSPVPAQPIYNIAGYGAVGDGATLNTAAIQR
                     AVDSCSAHGGGRIIVPAGRFVTGSVRLYSNMELCIEAGATLIGSPDNKDYLRQKDFGF
                     SGPGAGSRTGLLFAHDAENISITGHGVIDGRGDLFVYPDSLQRGTDFAPKYTRQGSGY
                     GDPKFGSADGPVLWKGDYESRPGVMLIFDGCKHIRVEDIGLHESPNWTIAFQSCEDVK
                     VRGISIDNDMSIPNSDGIDLYDSKSAVLSDCVINSGDDAIALISSSNIAVTNCILHSR
                     SSGIRIGYNVFNHRNSGGLLFDNIRIYDSNRGIGIFQRMDGDMSDMVFSNMIIQTRLH
                     TGEWWGHGEPIHISAVPGLGSKTVGKIRNVRFSHIVATAQTGIVLYGSPGSILKDITL
                     EDIDLRIERGPLSDSYGGNIDLRPANDLSLAIFRHVIPAVFATGVDGLDIRNFRVSWG
                     ADLPEYFQKEAKFPGCTRVSVQGWRNN"
     gene            complement(3607..4974)
                     /locus_tag="VGQ51_02695"
     CDS             complement(3607..4974)
                     /locus_tag="VGQ51_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035495.1"
                     /translation="MRPICLLPLVPLLFTASCKPILETEAGPAFHAPLPTSSECVVLG
                     VSDTIAIEGDTVGALNYSQTATSIPWDPNQIARYFQGEALHNGGNLVKITKYDWAEGE
                     LDQMTVHAAVYRVPDVRHYEKQIEWTPGRRLKLSDFKGPPDWDPTGRPLLHSYSGCDF
                     YLFSRSKFYCYYGWIDPASPDSLRLLLHEQGNFDLCEVYRRQLDEYVLRYENRGASQN
                     KTTEHIFREVYSAFLAKQAQYETDTDHGHDPVAQAEWTKRIAAELANPQHTHDPLFAA
                     DPDLVQQQNGNLVYALKPLPGKALVYIIRPKTISTPLPSRMLYDPLPLLLCAPYAYFI
                     NANSYTVGFKDSATNPIHGRTYTYTYLDPDKYTFSAVMNRQNEYEGVFRGHRYKKGPG
                     VELSVSAGKVYYLKLTTGETWFRFDKPRLELLSEKEGRALVRKCSLVSTGDDVDLPGL
                     ANPLE"
     gene            5080..6954
                     /locus_tag="VGQ51_02700"
     CDS             5080..6954
                     /locus_tag="VGQ51_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035496.1"
                     /translation="MKKYARLAALFCLAVLTNQRLSAQPELEPWGNLAGIRTDGQLFG
                     LATCLRVIDGDGHELVTAKERQDPHYSRTDKGWVVRTNLDSIYFTEMMADGGMGTAFI
                     SVDYRAHADMRLRKIYFTIDLPKGFAEHGINEWMDTVTHRKVDFRWVPPPTDSVWLEQ
                     EIASDKHIRINVPMRTGSVTSIDGKLMVFTITTSGPIDRRRANVHLNATTEGRPFDGV
                     GGNFRIQNTRLDPMVIDYCLKNIRVAWGRVELPWRWWQPVKDSDPLDSARAGHLNPAV
                     QRAMEMAHTLGAKKIPFVLSAWFPPDWAVVGPVSMRQPGPGHIWGNPLNHENDIAIYR
                     SIADYIAFLKEHDSVEPKYFSFNESDLGINVRQTAQEHDALIKGLGAYLASRGVRTRV
                     LLGDNSDATTWPFIGVAMQDTAARRWMGAVSFHSWRGWGTATLEHWADAATRLRLPLL
                     VAEGSIDAAAWHYPDIFKEPSYALKEIGLYTRLLALCQPESILQWQLTSDYSLLAGGG
                     IFGDMDELHPSQRFYNLKQLSMTPPGLFAMRVSCDRPNITVAALGDNKRRLYTLHLVN
                     DGATRDVVLTGLPADVKTLEIYTTNQMVSVKEGEPVHVLNGQARFTLAARSFVTLISR
                     "
     gene            complement(6991..7818)
                     /locus_tag="VGQ51_02705"
     CDS             complement(6991..7818)
                     /locus_tag="VGQ51_02705"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEV9035497.1"
                     /translation="MSLFTRMSNGWQLTLDSFAVLKENRQLILFPILSGISMMLVISS
                     FLVILLSSTGWDWEALRDQDNTTNYLFVFGYYLVNYFIIVFFNTALVHCTHLYFNGEK
                     PTVGQGLRFAFSRVGSILAWAVFAATVGTILRAIQDKVGALGKFITGLIGIVWSVATF
                     FVVPVLAYEHLGPLAAFKRSASLMKQKWGESLTASFSFGILQFLAILALVIPSFALGA
                     FVHPLAGIALFMLGVFALIVLFSAVKMIFISAVYHNINGDPVRHFNQQFADNLFVGK"
     gene            7961..>8772
                     /locus_tag="VGQ51_02710"
     CDS             7961..>8772
                     /locus_tag="VGQ51_02710"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEV9035498.1"
                     /translation="MKNLLLSALVLLLGACSEKPGNTEEGPAQHAAFHAANIKEQMIY
                     DRGVAAAIWGIPAVNFRLMYAAMEKLNGTFNEVVWWPKLLDWENQTLTPNQDVIYLMP
                     FVNTKEAGPMVLEIPPADSGALNGSVMNYWQAAIEDVGPGGVDKGHGGKYLVLPPGYD
                     MRKLPSGFIPMPSDTYQGYALLRSVLKSGSDADVAAAIRYARRIRVYPLSQASHPVPT
                     VFIDAGKDVYDATIPYDIRFYRLLDTMIQAEPWLDRDKAMIDPLKTIGIERG"
BASE COUNT         2026 a         2271 c         2567 g         1908 t
ORIGIN      
        1 accgaaaccc gggaaataga ctagtagctg ttggcagcgg gtgcggcagg tggcgctgca
       61 ctttggccgg ccggagccgg tggcggcggc gcggcgcttt gcccgcccgg accgtgttcg
      121 agaagttgtg acagcagctc cgttttcgga gtgaaaagga tgcgggtgcg atgttcgaca
      181 ggtagcgatg tcgtgccggc ggccgtcatc cgcgtcgggg agacctgatc gttttgcagg
      241 atacgaacga cggccgaggc ccgccggagc gcgagatccc agttgtcgtg gacgctgtcg
      301 gcaggcgcat tcaggctatc cacctggcct tcgaccgtaa attcgacatc gggtgcgttg
      361 gtgagcgccc gtgccagatg acccaggacc tgggttgcct tttcacttac cgtatagccg
      421 gcgctgtcgc taccgaaaag caggctgtcg gagagatcga cgtggaccgc ccctttttcc
      481 agcttgatgc tgacattctg ctccccgtaa ccgccgatgg tttgtttcag aatgaacaac
      541 agggcgagat tcaccgaatc acgatacgtt atcgcggaat gcagcgcgat catatagctg
      601 tcattggcgc ccatattgtc gagcgacctt ttgatgcttt ccgcctgggc gctgctgacg
      661 gcggatagct gctccatttg ttttctgagt tgcccgttga cctcattggc agcagtgact
      721 tgctggctca tctctttgtt atgttcctga agtgtcgatt tctctttgcc caggcgaatg
      781 ttgtcgtcct ggcaaccctt caacgtgcgc atcgactggg tgtacaggga gtcagaaaga
      841 tgggcctgct tttccatgtc gtggaatttc ccggaaggga cgcaaccgat aaaggaaagc
      901 gctacacaag gcaacaggat ggggaagtga cggaccgatc gcataaaaag gggtagtttg
      961 aagtgcacaa agatagggtc tttcagggga agatgatcat gaacaggtag accgcgaaca
     1021 gcaccagcag cacaataccc tgtaagatgg tcgtcctgcc cgtactcagc gaaatagcga
     1081 ccaccagcag cgtcagaaag aacagcaggg tggactttat atcgatgccc agcgctatgg
     1141 gcatcccggt gatgatcgat accgctgata cggttgggat ggtcagaccg atgctggcca
     1201 ctgcggaacc cagcgacaga ttgagcgcct tctggagatg atcgtgtcgc gcggcgcgaa
     1261 ccgccgccac cgactccggc agcagtacga tcatggcgat aatgacgcct accagcgaat
     1321 ggggggcgtt catcctgtcg acgagcctct ccagatcggg cgagagcgct tttgcgatga
     1381 tcaccacggc caggagacaa accagtaaga ggagaaagct gacagtcgtt tcgcgccggg
     1441 taggcggatg ctccgccgcg gcttcttctt cctttttttc cgactccgaa agaaagaaat
     1501 cgcggttcct gatattctgt acgataacga aggtggcata aagcacaagt gtgacgatac
     1561 ctacgaacag caattgagtg ttgctgtatt ccgggccgct ggctgaagaa gtgtaattcg
     1621 gaaggatcag cgtgagaacg gagatggaga ccagaacggt cagcgtcgaa cgaacccctt
     1681 ccagctcgaa tttctgcacc cggtatttaa gaccgccgat cagcagacac agacccatca
     1741 tgccgttgag gatgatcatc acggccgcaa acaccgtatc ccgggccagt ccttcatctc
     1801 ccccggcaaa catcaaagac acgatcaatg ccacctcgat gacggtgacg gcgatggcga
     1861 gtaccaggac accgaagggc tgaccgacgc ggtgagcgat gacctccgca tgatggacgg
     1921 ccgcgagaac gcttcctatc agacctgcca ccatcggtat cagccagata ccggaatgga
     1981 tccagtaatg caggccatat acgatccagg agaggaaggg aacataattc gaccatttca
     2041 gcatgcggcg gggtgttgcc atagaatcga ggttctccaa aattaaaatt ttaaaaccat
     2101 aactttaccc gcatgaagcc cgtttggaca atattcctcg tactcgtgct ttcttcaccg
     2161 gtgcccgcac agccgattta caatattgcc gggtatggcg ccgtcggaga cggcgccacg
     2221 ttgaacacag cggcgataca acgcgccgta gacagttgtt ccgcacacgg aggtggcagg
     2281 atcatcgttc cggccggtag gttcgtcacg ggatcggtca ggttgtacag caatatggaa
     2341 ctctgtatcg aggcgggggc gacgctcatc ggcagtccgg acaacaaaga ctatctacgt
     2401 cagaaagatt tcgggttcag cggtccgggt gcgggctcgc gtaccggcct cctgttcgcg
     2461 catgacgcgg aaaatatttc catcaccgga catggggtga tcgatggccg cggtgatctt
     2521 ttcgtctatc cggacagtct gcagcgcggc accgatttcg ccccgaaata tacgcggcag
     2581 ggtagcggct atggcgatcc gaaattcgga agtgcggacg gcccggtact ctggaaaggc
     2641 gactacgaga gtcggccggg tgtcatgctc atcttcgacg gttgtaagca tatccgggta
     2701 gaggatatcg gattgcatga atcgcccaac tggacgatcg cttttcagtc ttgtgaagac
     2761 gttaaagtac gcgggatcag catcgataac gacatgtcga tcccgaatag cgacggtata
     2821 gatctctatg actccaaaag cgccgtcctc tcggattgcg tgatcaattc gggggatgat
     2881 gcgatagcat tgatcagcag cagtaatata gcggtcacca attgtatcct tcattcccgg
     2941 tcgagtggta tccgaatagg atataatgtc ttcaatcacc gcaactccgg aggactgctg
     3001 ttcgacaata tccgcatcta tgattcaaac cggggtatcg gtatctttca gcggatggac
     3061 ggggacatga gcgatatggt cttttccaac atgatcattc agacgaggct gcacaccggt
     3121 gaatggtggg gacatggcga gccgatccat atctccgcgg tgcccggatt gggcagcaag
     3181 acggtaggga agatccggaa tgtgcggttc tcgcatatcg tagctacggc gcagacgggc
     3241 atcgtgctgt atgggtcgcc cgggagtatc ctgaaggaca tcacgctcga agatatcgat
     3301 ctccgtatcg aacgcggccc cctgtcggac agttatggag gaaatatcga tctgcggcct
     3361 gcgaacgatc tttcgctggc gatattccga catgtcattc ccgctgtttt tgccaccggg
     3421 gtagatggat tggatatccg gaatttccgg gtcagctggg gcgcggatct gccggaatat
     3481 tttcaaaagg aggcaaagtt tcccggatgc acgcgggtca gtgttcaagg ctggcgcaat
     3541 aattagtgtc cggctgggtg aaatgcacgg gccgggaggc gcccgatcac tccaggaggc
     3601 gcgcgatcac tccagcggat tggccagacc gggcagatca acatcgtcac cggtggacac
     3661 caggctgcat ttccggacga gggctcttcc ctctttttcg ctgagtaatt cgagccgggg
     3721 tttgtcaaat ctgaaccatg tctcccctgt ggtcagcttc agataataga ccttaccggc
     3781 cgaaacagaa agctccactc ccggaccttt tttataccga tgtccccgaa agactccctc
     3841 atattcgttt tgccggttca tgacggcgct gaaagtatat ttgtccgggt cgagatacgt
     3901 ataagtatag gtccgcccgt gtatcgggtt ggtggccgaa tccttaaatc ctaccgtata
     3961 tgaattggca ttgatgaaat acgcataagg agcgcacaac aacagcggga gagggtcata
     4021 gagcatcctc gaaggcagcg gcgtgctaat ggtcttcggc ctgatgatgt acacgagggc
     4081 cttgccgggc agcggcttga gcgcatagac aagattgcca ttttgttgct gtaccaggtc
     4141 gggatcggct gcgaacagcg gatcatgcgt gtgttgtgga ttcgccagct ccgcggcgat
     4201 cctttttgtc cattcggcct gtgcgacggg atcgtgtcca tgatcggtgt ccgtctcgta
     4261 ttgtgcctgt ttggcgagga atgccgaata cacttcccgg aagatatgtt ccgtcgtctt
     4321 attttggctg gcgccgcggt tctcgtaacg cagtacatat tcgtccagct gtcgccggta
     4381 gacctcgcaa agatcgaaat tcccctgttc gtgcagcagc aggcgaagcg aatcgggaga
     4441 ggccgggtcg atccatccgt aatagcaata gaatttggat cgcgaaaaaa gataaaaatc
     4501 gcaaccgcta tagctatgca ggagtggtct gcctgttggg tcccagtcgg gcgggccttt
     4561 gaaatcggac aattttagcc ttcggcccgg tgtccattcg atctgctttt cataatgccg
     4621 gacatcgggg acgcggtaga ctgcagcgtg gaccgtcatc tggtcgagtt cgccttcggc
     4681 ccaatcgtat ttcgtgatct tgacgagatt gccaccattg tggagcgcct cgccctggaa
     4741 ataccgggcg atctggttag ggtcccacgg aatggatgtc gccgtttggg aataattgag
     4801 cgcgcctaca gtatcgcctt caatggcgat ggtatcgctg acgccgagga ccacgcattc
     4861 cgaactggtg ggcagcgggg catgaaaagc aggaccggct tccgtctcta atatgggttt
     4921 gcaggaggct gtgaataata ggggaaccag cggcagcagg cagatagggc gcatggtgtt
     4981 gaaggttata atgggaagtt aggacaaaac cggccggagt gtccttaaaa accggttaaa
     5041 gacttctaat cgggttgtta atgattgtat ttttatccta tgaaaaaata tgcccgtctc
     5101 gccgccttgt tttgcctcgc ggtcctaacc aaccaacgcc tctcggccca gccagagctg
     5161 gaaccctggg gcaacctcgc tggtatccgg accgatggtc agcttttcgg gctggccacc
     5221 tgtttgcgcg tgatcgatgg cgacgggcac gagctcgtca ctgccaaaga gcggcaggac
     5281 ccacattatt cgcggacaga caaggggtgg gtcgtgcgca cgaatctcga cagcatttat
     5341 tttacagaga tgatggcgga cgggggcatg ggtacggctt ttatcagtgt cgactacagg
     5401 gcacatgcag atatgagatt gaggaagatc tattttacca tcgatctgcc gaagggtttc
     5461 gccgaacatg ggatcaacga atggatggac actgttacgc atcggaaagt ggatttccgg
     5521 tgggtgccgc cccccacgga cagcgtttgg ctggagcagg agatcgccag tgacaaacat
     5581 atccgcatca atgtaccgat gaggacgggg tcggtcactt ccatcgacgg gaagctcatg
     5641 gtcttcacca tcaccacaag cggccccatc gaccgacgcc gcgcgaacgt tcatctgaat
     5701 gccacaacgg aggggcggcc tttcgacggc gtcggaggca atttccgcat ccagaatacc
     5761 cggctggacc cgatggtgat cgactattgt ctgaaaaata tacgcgtggc ctgggggagg
     5821 gtagagcttc cgtggcgctg gtggcagccc gttaaggaca gcgatccact ggactcggca
     5881 agggccggtc atctcaatcc ggcagtacaa cgggcgatgg aaatggcgca tacgctcggc
     5941 gccaaaaaga tcccctttgt cctttctgcc tggttccccc cggactgggc ggtcgtcgga
     6001 cccgtgagca tgcggcagcc cggtcccggt catatctggg gcaatccgct gaaccatgag
     6061 aacgacatag ccatctatag atccatcgcc gactatatcg ccttcctgaa agagcacgac
     6121 agcgtcgagc cgaaatattt ttccttcaat gagtccgatc tggggatcaa tgtccgtcag
     6181 acagcgcagg aacatgacgc gctgatcaaa gggctggggg cgtatcttgc ttcacggggt
     6241 gtgcggacac gcgtattgtt gggggataat tccgatgcga caacctggcc gttcatcggt
     6301 gtggccatgc aggacaccgc ggcccggcgt tggatggggg cggtatcttt tcattcgtgg
     6361 cgcggttggg gtaccgctac cctggagcac tgggcggatg ccgcgacaag gcttcgcctt
     6421 cccctgttag tagccgaagg cagcatcgac gcggccgcat ggcattatcc cgacatcttt
     6481 aaagaaccga gctatgccct gaaggaaata gggttgtata ctcgtctgct ggctctctgt
     6541 caaccggaaa gtatcctgca atggcagctg acctcggatt attcgctgct ggcaggtggc
     6601 gggatcttcg gcgatatgga cgagcttcat cctagccagc ggttttacaa tctgaaacag
     6661 ctgtctatga cgcctccggg actatttgcg atgcgcgtga gctgcgaccg gcccaatatt
     6721 accgtagccg cactgggcga taataaacga cggctttata ccctgcatct cgtcaatgac
     6781 ggagcgacac gtgatgtcgt cctgacagga ctgccggcgg acgtgaagac tctggagatc
     6841 tatacgacga accagatggt ttcggtaaaa gaaggagagc ctgtccacgt gttaaatgga
     6901 caggctcgtt tcacgcttgc cgcgcgaagt tttgtaacac tgatcagccg ataaacaccg
     6961 cggccgcccg aagggcggcc gcgtctcaat ctattttccg acgaaaagat tatctgcaaa
     7021 ctgttggttg aaatgtctca cggggtcacc gttgatatta tgataaacgg cactgatgaa
     7081 gatcatcttt acagcgctga aaagcacgat gagcgcgaag acgcccagca tgaacaatgc
     7141 gatgcccgcc agaggatgaa cgaatgcacc caaagcgaac gacgggatca ccaatgccag
     7201 gatggccagg aattggagga tgccgaaact aaagctggct gtgagacttt ctccccattt
     7261 ttgtttcatc agcgaagccg agcgtttgaa agcggccagc ggtccgaggt gttcgtaagc
     7321 gagaacgggc accacgaaaa aggttgctac ggaccaaacg attccgatca ggcccgtaat
     7381 gaatttaccg agggcgccga ccttgtcctg tatcgcgcgc aggatagtgc ccacagtcgc
     7441 cgcaaaaact gcccatgcca ggatcgatcc caccctgctg aacgcgaacc gaagcccttg
     7501 tccaacggta ggtttttcac cgttgaaata taaatgggtg cagtgaacca gcgctgtatt
     7561 gaaaaagacg atgatgaaat agttgaccag gtaatagccg aaaacgaaca ggtaattggt
     7621 ggtgttgtcc tggtcgcgca gcgcctccca gtcccagcct gtagaagaca ggaggatcac
     7681 caaaaaggac gagatgacca gcatcatcga aataccggac aggatcggga aaaggatcaa
     7741 ctgacgattt tctttgagta cggcaaagct gtccagcgtt agctgccagc cattcgacat
     7801 acgcgtaaat aggctcatag taaaggattg atttaattgc ccaatataag gcaaagtgct
     7861 ggaaattgca agcggctaag tgactgatat ataggcgttt tttgcctgca tttttcctcc
     7921 agaattggcg gagcggctgg tcggcgggtg tcttttcggt atgaaaaatt tattattatc
     7981 ggcccttgtg cttctgctcg gagcctgcag cgaaaaaccc ggcaatacgg aggagggccc
     8041 ggctcagcac gcagccttcc atgctgcaaa tatcaaagaa caaatgatct atgacagggg
     8101 tgtcgccgcg gcgatctggg ggataccggc cgtcaatttc cggctgatgt atgcggcgat
     8161 ggaaaaattg aacggcacgt tcaacgaggt ggtctggtgg ccgaaattgc tcgactggga
     8221 aaaccagaca ctgacgccca accaggatgt gatatatcta atgccgtttg ttaatacgaa
     8281 agaggcggga ccgatggtac tcgagatccc gcccgccgat agcggggcac tcaatggcag
     8341 cgtaatgaat tactggcagg cagccatcga agacgtcggc ccgggtggtg tcgacaaagg
     8401 gcatggcggc aaatacctgg tattgccgcc gggttatgat atgaggaaac tgccttccgg
     8461 ctttattccc atgccatcgg atacctatca gggatatgcc ttactgcgga gcgtcctgaa
     8521 gagcggcagc gatgccgatg tggcggccgc gatccgttat gcgcgaagga tcagggtcta
     8581 tccactgtcg caggcatccc atccggtgcc taccgttttt atcgatgccg gcaaggacgt
     8641 ttacgatgcg accataccct acgatatccg gttctaccgt ctgctcgata cgatgataca
     8701 ggcggagccg tggctcgacc gggataaggc catgatcgat ccattgaaaa ccatcggcat
     8761 cgaaagagga aa
//
LOCUS       DASYSV010000089         6693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1813220, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000089 DASYSV010000000
VERSION     DASYSV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813220"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VGQ51_02715"
     CDS             <1..213
                     /locus_tag="VGQ51_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035499.1"
                     /translation="EGIIDSGTLTIYIRSSLPYLTAWYPFPSYVFPDGSTVTLRRVSE
                     QPGKIVLASVGATTPPMNYFISLSVK"
     gene            complement(645..1856)
                     /locus_tag="VGQ51_02720"
     CDS             complement(645..1856)
                     /locus_tag="VGQ51_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9035500.1"
                     /translation="MVMRNSFIVAIGTFLLVACQGKKKEQITAMATRPDTMYTCSMHP
                     QVMQDRPGKCPICRMQLIPVKMGVDEEGTVALSDQQMRLGDIRVDTVEATAVGDRMVL
                     SGTVTVDETRSSAVSARIGGRVERLYYKATGEYLHKGDKLYDLYSEELNNAKQEYLLA
                     LDRLDNLKGGLVDLKQLAEAARNKLLLWGMSESQVAELARTRQAGNVTSFYSPVSGYI
                     SSLESHEGDYLSTGTVLVRLVDLSIVWVEAQVYAAQLSALDRNGKAEVHFPDLPGKEF
                     TGRIGLVNPELDPAGRTNLVRVEVQNAGSLLRPGMPAEVMVTSRERHSLTLPVGAVLH
                     GKRGDVVWVATGHNRFRPVMVSLGLEADGRVEIRSGVKAGDAVVTEGAYLVNSEYVFK
                     YGQDARAAMNM"
     gene            complement(1856..3148)
                     /locus_tag="VGQ51_02725"
     CDS             complement(1856..3148)
                     /locus_tag="VGQ51_02725"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9035501.1"
                     /translation="MKSIPYGVTLAVAVIILAGCGRGSSGKTPTKDSGMQGMAVTDSE
                     IAAVPVAKTAWTCPMHPEVIKDKPGTCPICGMDLERKETTAPHVKNIGLEDLVQPTNG
                     FVVSSVATTVMRRDTVRPELAVFGTVGYDTRLQNTIAARVSGRIERLYVRYRYQHVQH
                     GQRIMDIYSPELETGEQEYLYLLKNDPGNAAMIGAARQRLLLLGVNGAQLEQVARTGK
                     ASATLSVFSPYTGHIHEAGNTMPGGSLSGTAGVGMTEELPVKEGMYVEKGQPVFQVFN
                     MDRGWVLLSLYPGQEALVRKGDPVEVVPETAPDKAFAGRVDLVEPVYRQGDWSLTVRI
                     YFDNSRRDIPIGSAVKATIGPAGVTGNWLPKAAVLSLGMDKVVFVRQDSGFRAHQVVT
                     GLESGHRVQILAGLSDHDLVAANAQFLMDSEDFVKVKN"
     gene            complement(3155..4405)
                     /locus_tag="VGQ51_02730"
     CDS             complement(3155..4405)
                     /locus_tag="VGQ51_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9035502.1"
                     /translation="MKKIICLIGMLGVVAARGQTIMSLDSIFSVISRNNPALRSSDAS
                     ARSLDEAAKGARSWEAPMASTGWWMTPYDPSLWKRQANGASGMGQYMVSAEQLFPNRR
                     SQEAQQKYLGALSSVERTKKGATMNELYASARQAYFQWLVAKRREMVLDDNGKLLDFM
                     IRDAELKYKNNLGKIGAYYKAKAALGNLENRRIALDNIMEQQRIKLNTLMNRDKVAAF
                     DIDTSYKVMDVAGLGADSAGLLDSRSDIRAVSESIRVTGLEQDAERARLKPEFGVRYD
                     HMFGFGGTPMQYSLMATVRLPMAAWASRGVKANVESLKWKALSLEEERDAMVTEATGA
                     AYGLKRAILAKQRQVHVLAEQVLPALRKNFETMELAYEQNTEELFTLYDAWETLDSSQ
                     MEYWDIVESLLMMQVELKRVLEIK"
     gene            complement(4410..>6693)
                     /locus_tag="VGQ51_02735"
     CDS             complement(4410..>6693)
                     /locus_tag="VGQ51_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEV9035503.1"
                     /translation="MADVQRGLPAGVTFRVAYDRSELIKAAVGNVKSKLIEELVIVSL
                     IVLLFLLDVRSALSIIIQIPISIATSFILLNAFGLSSNIMSLTGIALAIGVIVDNGII
                     MSENAYRHLADEQARLAVARGGVGSLPLLTGGNRLDIIRKACIQVSRGVFFSTVIIIA
                     SFLPVFLLTGQEGRLFHPLAYTKTFILLVDAILVVTLAPVLISLFMRGRFRREGANPI
                     NRLLERVYERVLRACLRVRWMTLGVNVLVLAACIPLLLSLGREFMPPLDEGMILFMPV
                     TLPDVSNAEIKRLLQVQDKIIASEPEVANVLGKAGRANTATDNSPISMVETIVLLKPK
                     AQWRPGVTKDSIIRELNSKLQIPGVTNGWTQPIINRINMLSTGIRTDVGVKVYGQNLD
                     TINSIAQRIRAALNGIPGVSDLYVDPITGGKYLDITVKREEIGRYGLTVDDVNTIVES
                     ALGGMKLTTTVEGRQRFSVNARYGQDFRDNLTALRRLQVPTPSMGPIPLETVADIQLT
                     EGPPMINSENAALRGTVLFNVRGRDLAGTVKEAQQRLDKALTALPKGYYIEWSGEYEN
                     LIRSERTLTWILPVVLGIIFAAMYMAFRSMREAFFSLISIPFALIGGALMVYLWGVNL
                     SVAVAVGFIALFGLAVETGIVMVIYLNDAMSQLVATSGNSREAISRTALREAVIAGAA
                     KRLRPKLMTVSVSLFALIPILWSNGVGSDVMKPIVLPMIGGVLTSATHILLVTPLIFL
                     MTKEYELRKYGRIEVMEAKK"
BASE COUNT         1474 a         2228 c         1431 g         1560 t
ORIGIN      
        1 gaagggatta tcgacagtgg aaccttaacc atttatatcc ggtcgtcgtt accttatctt
       61 acggcctggt atccctttcc tagctatgtc tttccggacg gttccactgt aacgctgaga
      121 agggtgtcgg agcagccggg aaagatcgtc ctcgcctccg tgggtgctac gacaccaccg
      181 atgaattatt ttatttccct ttcagtaaaa tagacatctc cgtaatcagc ccgcattgat
      241 tttactgggt cgggcaatgc tgccttcatc gcctggaaat ccgccactaa cagggcagcc
      301 gttccaggcg gttctgtagt aacgaaagcg atactcggca ttccgatatt ggaaataaat
      361 gaactcaggg aaaacatgaa agttattctt cttgccagga aacggttaat tcagtgtccg
      421 ggcgttaaat tgacaagttg ctgaaaacca ataaatcaat catgatttgg ttagataatt
      481 ttccccgtag gggtacttct tcttttgagg tcgtcactaa aaatgacgac cctttttcat
      541 ttaattcaca acatactata gattacgggt agacgcgatg cgggcaaata gtatatcttc
      601 agttttgact ccaagttaga acacctaatc atggttatgc aattttacat gttcatcgcc
      661 gcccttgcat cctgcccata cttgaacaca tactcactat ttaccaaata ggccccttcc
      721 gtaaccaccg cgtcccccgc cttcactccc gaccgaattt ccacccggcc gtccgcctcc
      781 agaccgagcg aaaccattac gggccggaac cggttatgcc ctgtcgctac ccaaaccaca
      841 tcaccacgct tgccgtgcaa gaccgcccct acgggcaagg tcagcgaatg ccgttccctg
      901 ctcgttacca tcacctctgc cggcatcccg ggccgcaata ggctcccggc attttgcacc
      961 tcaacccgca ccaggttggt ccgccccgcc gggtccagct ccgggtttac aaggccgatc
     1021 cggcccgtaa actccttacc cggtaaatcc gggaaatgca cctcagcctt cccattccga
     1081 tccaacgccg ataactgcgc agcatatact tgcgcttcta cccatacgat ggacaagtcc
     1141 accaaccgaa ccagcacggt acctgtcgaa agataatcgc cctcatggct ttccagagaa
     1201 gaaatatatc cgctcaccgg gctataaaac gaagtgacat tccccgcctg ccgcgttcgc
     1261 gccagttccg ccacctggct ctcgctcata ccccacagca acagcttatt ccgcgccgct
     1321 tccgcaagct gcttcaggtc caccagccca cccttcagat tatccaatct gtcaagggcc
     1381 aacagatatt cttgtttggc attgttcaat tcctcgctat acaaatcgta tagcttatcc
     1441 cccttatgca ggtattcccc tgtcgcctta tagtacaacc tttcgacacg tcctccgatc
     1501 cgggcgctca ccgccgaact acgggtctca tccaccgtta ccgtccccga cagcaccatg
     1561 cggtcgccaa ccgcggtggc ctccaccgta tccacacgga tatcccccaa tcgcatctgc
     1621 tgatcgctca acgccactgt cccttcctcg tccactccca tctttaccgg tatgagctgc
     1681 attcgacaga tcgggcattt acccggccta tcctgcatca cttgcggatg catagaacat
     1741 gtgtacatcg tgtctggcct cgtggccatc gccgtaattt gctctttttt ctttccctgg
     1801 caagcaacca acaagaaagt ccctatcgcc actataaaag aatttcgcat aaccatcagt
     1861 tttttacttt tacaaaatct tcgctatcca tgagaaactg tgcattcgct gccaccaagt
     1921 catggtccga aagccctgct agtatctgca cccgatgtcc actctccaat cccgtcacca
     1981 cctgatgcgc ccggaacccg ctatcctgcc gcacgaaaac aaccttatcc attccaagtg
     2041 acaagaccgc cgccttcgga agccagttcc ccgtcacccc agcaggcccg atcgtcgcct
     2101 tcaccgcgct gccgataggg atatcccgtc tactattgtc aaaatatatg cgaaccgtca
     2161 gcgaccagtc cccctgccgg tacacaggct ctacgagatc cacccgccct gcgaacgctt
     2221 tgtccggcgc cgtttccggc accacttcca ccgggtcgcc tttccgcacg agcgcctcct
     2281 ggccgggata caaactcaac agcacccagc cccggtccat attgaaaacc tggaagaccg
     2341 gctgcccctt ctcgacatac atcccttctt ttaccggcag ttcctccgtc atgccgaccc
     2401 ctgctgtccc actcaaactg ccgcccggca tggtgtttcc agcctcgtgg atatgtccgg
     2461 tataagggct aaagacactc agcgttgccg acgccttccc cgtccgggct acttgctcca
     2521 actgtgcccc gttcaccccc agcaacagca aacgttgtcg cgcggccccg atcatcgctg
     2581 cattaccggg atcgttcttc aagagataca aatattcttg ttcccccgtc tccaattccg
     2641 ggctgtagat atccatgatc cgttggccgt gctgaacatg ctggtaccgg taacgcacat
     2701 acagtctttc gatccgtccc gaaaccctgg cagcgatcgt attttgcaga cgggtatcat
     2761 atcccaccgt cccgaacacc gccagttcgg gtcgaaccgt atcccgacgc atcaccgtgg
     2821 ttgcgacgga cgacaccacg aacccgttgg tcggttgtac caaatcctcc aggccaatat
     2881 tctttacatg cggtgccgtc gtctccttcc tttccaagtc cattccacag atggggcagg
     2941 ttcccggctt gtcctttatg acttccgggt gcatcgggca cgtccatgcc gtttttgcta
     3001 ctggtaccgc ggctatttcc gaatcggtca ccgccattcc ttgcatccca gaatccttcg
     3061 tgggcgtctt ccccgacgaa cctcttccgc aacccgccaa tattatgaca gccaccgcca
     3121 acgtcacgcc atatggaata gatttcataa acgatcattt aatctccaat actcttttta
     3181 gttccacttg catcatcagc aaactctcca caatgtccca atactccatc tgcgaggaat
     3241 ccagcgtctc ccaggcgtca tagagcgtaa acaactcctc cgtattctgt tcatacgcca
     3301 gctccatcgt ctcgaaattc ttacgcaatg ccggcaatac ctgctccgcc aatacgtgca
     3361 cctgacgctg tttcgccagg atggcccgct tcagcccgta agcagcgccc gttgcctcgg
     3421 tcaccatcgc atcccgctct tcctcgagag acaacgcttt ccacttcagg ctctcgacat
     3481 tcgcctttac cccccgcgat gcccatgctg ccatcggaag ccgtacggtc gccatcagtg
     3541 aatactgcat gggcgtgccg ccaaagccga acatatgatc gtaccgcacg ccgaactccg
     3601 gcttcaacct ggcccgttcc gcatcttgtt ccagccccgt cacccgaatg ctttcgctca
     3661 ccgctcgaat gtcgctacga ctatccaaca gacccgcact atcggcgcct agcccggcaa
     3721 cgtccataac cttatagctc gtgtcaatat caaacgcagc cactttgtcc cggttcatca
     3781 gcgtattcag tttgatccgc tgttgctcca ttatattgtc caatgcaatg cggcggtttt
     3841 ccaggtttcc cagcgccgcc ttcgccttgt aataagcgcc gatctttccc aggttgtttt
     3901 tatactttaa ctccgcatcc cggatcataa agtccagcag cttgccgtta tcatccagca
     3961 ccatctcccg gcgcttagcg accaaccatt ggaaataggc ttgcctggct gaagcataga
     4021 gctcgttcat tgtagcccct ttctttgtgc gttctactga cgacaatgca cccaaatact
     4081 tctgctgcgc ctcctggcta cgcctgttag gaaatagctg ttccgccgac accatgtatt
     4141 gccccatacc cgatgcccca ttcgcctgcc gcttccacag agaaggatcg taaggcgtca
     4201 tccaccaacc cgtgcttgcc ataggcgctt cccagctacg cgctcccttc gccgcttcgt
     4261 ccaggcttcg tgccgaggca tccgatgacc ggagcgccgg attgttacgg ctaatcaccg
     4321 aaaaaatgct gtccaaactc atgatcgtct gcccccgtgc cgcaacaacg cccagcattc
     4381 ctatcaaaca aataatcttt ttcatggtat tacttttttg cctccattac ttcaatacgc
     4441 ccatatttcc gcagctcata ctccttcgtc attaaaaata tcaagggtgt caccagcagg
     4501 atatgcgtcg ccgatgtcaa caccccgccg atcatcggca ggacgatggg cttcattaca
     4561 tcgcttccga ccccattgct ccacagtatc gggatcagtg caaacagcga caccgatacc
     4621 gtcatcagtt tgggtcgtag ccgttttgcc gctcccgcga tcactgcttc ccgcaaggcc
     4681 gtccgcgaaa tagcctcccg tgaattgccg ctcgtagcca ctagctgaga catcgcatca
     4741 ttcaggtaga tcaccatcac aattcccgtc tccaccgcca gcccgaataa cgcaataaaa
     4801 cccaccgcaa cggccaccga caggttcacg ccccacaggt ataccatcag ggccccgccg
     4861 atcagcgcga agggtatcga gatcaacgag aagaacgcct cccgcatcga cctgaacgcc
     4921 atgtacatcg ccgcaaagat gatccccagc acgaccggca ggatccaggt caatgtccgc
     4981 tcgctgcgga tcaggttctc atactctcca ctccattcga tataatatcc tttgggtaag
     5041 gccgtcagcg ctttgtccag ccgctgctgc gcctccttta ccgttcccgc caggtcgcgg
     5101 ccccgcacgt tgaagagcac cgttccccgc agcgccgcgt tctccgaatt gatcatgggc
     5161 ggtccctccg tcagttgtat atccgccaca gtctccaagg gtattggacc catcgatggc
     5221 gtaggcacct gtaatcttct gagtgccgtt aaattatccc ggaaatcctg gccgtatcgt
     5281 gcattcaccg aaaaccgctg ccggccctcg accgtcgtcg tcaatttcat gccgcccagg
     5341 gcgctctcga caatggtatt gacgtcatca acagtcagcc catatcggcc gatctcctcc
     5401 cgcttcaccg taatgtccag gtatttgcca cccgtgatcg ggtctacata gaggtccgat
     5461 acaccgggaa tgccgttcaa cgccgcccgt atccgctgtg caatgctatt gatggtatcc
     5521 aggttctgtc cgtagacctt cacccccaca tccgtgcgta tgccggtcga aagcatgttg
     5581 atccggttga tgatcggctg cgtccatccg ttcgtcaccc cggggatctg caacttgctg
     5641 ttcagctccc ggatgatgct gtccttcgtt acaccaggcc gccactgggc cttaggtttc
     5701 agcagcacga tcgtctccac catgctgatc ggtgaattgt ccgttgccgt atttgcccgg
     5761 ccggccttcc ccagtacatt cgccacttca ggctcggaag cgatgatctt atcctggacc
     5821 tgtaacagcc gtttgatctc tgcattcgac acatccggta aggtcaccgg cataaatagg
     5881 atcataccct cgtccaatgg cggcatgaat tcccttccca ggcttaaaag caggggtata
     5941 caggctgcaa gcaccagcac gttcacgccg agcgtcatcc agcgaactcg caaacaggcc
     6001 cggagcaccc gttcatatac tcgttccagc aggcgattta tcggattagc gccctcccgg
     6061 cgaaaccgcc cccgcataaa caacgagatc agcaccggtg ccagcgttac caccaggatc
     6121 gcatcaacca gcaggataaa tgtctttgta tatgctaagg gatgaaacag cctgccttcc
     6181 tgccctgtca gcaggaacac cggcaagaaa gaagcaataa tgattaccgt agaaaagaac
     6241 accccgcgtg atacctggat acaggcctta cggataatgt ccaatcggtt acctcccgtc
     6301 agcaggggca gcgaacccac gcctccccga gcaaccgcca gcctcgcttg ttcgtcggcc
     6361 agatgccggt acgcattctc cgacatgatg atcccattgt cgacgatcac tccgatggcc
     6421 aacgcaatgc ccgtcaacga catgatattt gatgacagcc caaaggcatt cagcaggata
     6481 aaactcgtgg caatgctaat tggtatctgg atgattatgc taagtgcact gcgcacatcc
     6541 agcaggaaca gcagcacgat cagcgacacg atgaccagtt cctcgatcaa cttgctcttc
     6601 acattcccta cggccgcctt gatcaattca ctgcggtcat aggccacccg gaacgtcacc
     6661 cccgccggca gtcctcgttg cacatccgcc atc
//
LOCUS       DASYSV010000090         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_402819, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000090 DASYSV010000000
VERSION     DASYSV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402819"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGQ51_02740"
     CDS             <1..463
                     /locus_tag="VGQ51_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035504.1"
                     /translation="QFDAASKSYDATIQNSPDKRMKENGYYNKGVAMIKQKKLQESID
                     AWKQALKLDATDADARDNLERALRQLKQQQQQQQQNQNEQKKNQKDQKKNEKKNQDQQ
                     QQQQEPKPQPSRLNKQEVEQLLKALEQKENDLHNKMQQNKMHIPNQPDKDW"
     gene            537..2426
                     /gene="ispG"
                     /locus_tag="VGQ51_02745"
     CDS             537..2426
                     /gene="ispG"
                     /locus_tag="VGQ51_02745"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEV9035505.1"
                     /translation="MQSYCNSLTAYSRLKTLEVKIGDLLLGNFHPIRVQTMTTTDTMD
                     TNATVEQSIRCIRAGSELIRITAPSKKEAENLLNIKNELRRRGYTTPLVADIHFTPNA
                     AEIAARIVEKVRINPGNYIDKKKFEHLEYTDAEYREEIDRIRQRFTPLIKICREYGTA
                     MRIGTNHGSLSDRIMSRYGDTPMGMVESAMEFLRVARDESYHNIVLSMKSSNPQVMVQ
                     AYRLLIRNMNEEFGVCYPLHLGVTEAGDGEDGRIKSAIGIGTLLEDGIGDTIRVSLTE
                     DPEFEIPVCKELVQRYESRQTAAGIPAIEKIPYSPFEYRRRVTTPVGPIGGQHVPVVV
                     ADFSGSQTIEREHLRAIGYAYDAATDKWNISDAAADLIYTGNKTIPFDLPGTLRPIRD
                     ADSIHFIHIDSADPDAPALAASFRDNATAVLCISSSHPQPVQSVRRLIMHLMTNDIRC
                     PVILKVESRQETIDRQLIHFSTETGALLLDGLGDGVWLKNHPEKIVDTKISGRTYLET
                     RNNHQFLNNTAFSILQATRTRISKTEYISCPSCGRTLFDLQETTAKIRSVTHHLKGVK
                     IAIMGCIVNGPGEMADADFGYVGSGPGKITLYRGKEVVKRGVDSAIAVDELINLLKES
                     KAWID"
     gene            2444..>2921
                     /locus_tag="VGQ51_02750"
     CDS             2444..>2921
                     /locus_tag="VGQ51_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEV9035506.1"
                     /translation="MNRIDRLHAILTHLQSKRRVTAQEMADRFNISLRTVYRDVKALD
                     ESGVPVIGEAGTGYTIMEGYRLPPVMFTQEEASALLLGAKLAERFTDGSVKRHFQSAL
                     FKIKAVLRSPDKEYVDSLTEHIEILTRYTPDSDSPQQYLSLMQQAIVHKKTVFIQYR"
BASE COUNT          797 a          847 c          738 g          539 t
ORIGIN      
        1 ccagttcgac gccgcctcga agtcgtatga cgctaccatc caaaacagtc ccgacaaaag
       61 gatgaaggaa aatggctatt acaacaaggg cgtcgccatg atcaaacaga aaaagctgca
      121 ggagagcatc gatgcctgga aacaggcgct caagctggac gccactgatg ccgacgcaag
      181 ggataatctc gaaagggcgc tgagacagtt gaaacaacag caacagcaac aacagcaaaa
      241 ccagaacgag caaaaaaaga accaaaaaga ccaaaagaag aacgaaaaaa agaaccagga
      301 tcaacagcaa cagcagcaag agccaaaacc tcagcccagc cggctgaaca aacaggaagt
      361 ggaacagctg ctaaaggcgc tcgaacaaaa ggagaacgat ctgcacaaca agatgcagca
      421 aaataagatg catatcccca atcagcccga taaggattgg taggccacgc acctgcacgc
      481 cgccagcccg tgcgcggccg ctgcttcccg ccgattcttt gtacctttac actcctatgc
      541 aatcctattg taactccctt accgcctact cccggcttaa aacgctggag gtgaagatcg
      601 gcgacctgtt gctgggcaat ttccatccta tccgcgtgca gaccatgacc accaccgata
      661 cgatggatac caacgcgact gtggaacaat cgatccggtg tatcagggca ggctctgagc
      721 tgatccgtat cacggcgccc agtaaaaaag aggcggagaa cctgttgaac ataaagaacg
      781 aacttcgccg cagaggctat accactcctc tcgtcgcgga tatccatttc acccccaatg
      841 cagcggagat cgcggcgcgc atcgtcgaga aggtccgtat caatcccggt aactatatcg
      901 ataagaaaaa attcgaacat ctcgaataca ccgacgccga ataccgggaa gagatcgacc
      961 gcatccggca acggttcact ccgctgataa agatttgcag ggaatacggg acggccatgc
     1021 gcataggcac caaccatggc tctctcagcg accggatcat gagccgctac ggcgacaccc
     1081 cgatggggat ggtggagagt gccatggaat tccttcgggt agccagggac gaaagctatc
     1141 acaatatcgt cctgagcatg aagtccagca atccgcaggt gatggtacag gcctaccgcc
     1201 tgctgatccg caatatgaat gaagagttcg gcgtatgcta tccgcttcac ctgggcgtta
     1261 cggaagccgg cgacggcgaa gacggacgca tcaaatcggc catcggcatc gggacacttc
     1321 tcgaagacgg catcggcgat acgatccgcg tgtcgttgac ggaggacccg gaattcgaga
     1381 tacctgtctg taaagagctg gttcaacgat atgagagccg ccagacagcc gccggtatcc
     1441 ccgcaatcga aaagataccc tattcgccat tcgaatatcg ccgccgcgtt acgactcccg
     1501 tgggtcctat cggcggtcag catgtccccg tagtagtcgc agatttcagc gggtcgcaaa
     1561 ccatcgaaag ggaacacctc cgcgctatcg gctatgccta tgacgccgcc accgataaat
     1621 ggaatatctc cgatgctgcc gccgatctta tttacaccgg gaacaagacc atcccattcg
     1681 acctgcccgg cacgctccgg ccgatccgtg acgccgacag catccatttc atccatatcg
     1741 atagcgctga ccccgacgcg ccggcactcg ccgcatcctt cagggacaat gccacagccg
     1801 tactttgcat cagctcctca catccccagc cggtgcaatc cgtccgtcgg ctgatcatgc
     1861 acctgatgac caatgatatt cgctgtcccg tgatcctgaa agtagaaagc cggcaggaga
     1921 ccatcgaccg gcagctgatc catttttcta ctgaaacagg tgcgcttctc ctggatggct
     1981 taggcgacgg cgtctggctg aagaaccatc cggaaaagat cgtggatacg aagatcagcg
     2041 gtcgcacata cctggagacg aggaacaatc atcaatttct caataatact gccttctcca
     2101 tcctgcaggc cacgcgtaca cgcatttcca agacggaata tatttcctgt ccctcctgcg
     2161 gacgtactct tttcgatctg caggagacca cggcgaagat ccgctcggtg acccatcatc
     2221 tgaaaggcgt gaagatcgct atcatgggtt gtatcgtcaa tggacccggc gagatggccg
     2281 atgccgactt cggctatgta ggcagcggac cgggaaagat caccctctat agaggaaaag
     2341 aggtcgtcaa aagaggagtg gacagtgcca tcgcggtaga cgaattgatc aatttgttaa
     2401 aagaaagcaa ggcgtggatc gattaaatta aacagttgaa ccggtgaacc gcatcgatag
     2461 actacatgct attctcaccc atctccaaag caaacggcgg gtgactgcac aggaaatggc
     2521 agaccgtttc aatatcagcc tgcgcaccgt ctatcgcgac gtcaaggcgc tggatgaatc
     2581 cggcgtaccg gtcatcggtg aggcgggcac cggctatacg atcatggaag gctaccggct
     2641 tccgccggtg atgttcaccc aggaagaggc cagcgcattg ctgctgggcg ccaaactggc
     2701 cgagcggttt accgacggct ccgtcaaacg gcatttccaa tccgctcttt ttaagatcaa
     2761 agcagtcttg cgtagccccg acaaggagta tgtcgacagc ctgaccgaac acatcgagat
     2821 cctcacccgc tatactcccg acagcgactc tccccagcaa tatctttccc tcatgcagca
     2881 ggccatcgtc cacaaaaaga cagtatttat ccaataccgg t
//
LOCUS       DASYSV010000091         6238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_789520, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000091 DASYSV010000000
VERSION     DASYSV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789520"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2770)
                     /gene="uvrA"
                     /locus_tag="VGQ51_02755"
     CDS             complement(<1..2770)
                     /gene="uvrA"
                     /locus_tag="VGQ51_02755"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV9035507.1"
                     /translation="MEEMIEVSGARVHNLKNIDIRIPKNQLVVITGISGSGKSSLAFD
                     TIYAEGQRRYMESFGAYARQFIGDMERPDVDKITGLSPVISIEQKTTNKNPRSTVGTV
                     TEIYDFLRLLYARIGIAHSYNTGKVMTKFSEEEILDNVFKKFRNKKISILAPLVRGRK
                     GHYRELFEDIRKKGYLKVRVDGEVKDLVPKMQVDRYKIHDIEVVIDRLAVTDEMKARL
                     GQSVQKALQMGKDLLFLLLNDDNKVIQYSKQLMCEDSGISYEEPSPNSFSFNSPYGAC
                     PTCKGLGAVYQIGMDAIIPDRSKSINEGGIAPLGGEREAYMFRQVQALAKKNKFSLDT
                     PIEDMSKKALNLVLYGNETGEATLEVEFNDAANAETGQVYASDYEGIVNALKRWFAGG
                     TTDSLREWVEQFMELKTCGTCGGTRLRRDSLWFRVGEKNIAELSSMNLNRLVDWFAGI
                     EKKLSDKQNIIAKDILKEIRERLRFLTDVGLTYLTLDRPSRTLSGGESQRIRLATQIG
                     SQLQGITYILDEPSIGLHQRDNHRLIQALKNLRDIGNSVLVVEHDKDIMLAADYLVDI
                     GPQAGFHGGRVVAQGRPPALLRLNTLTADYLNGRRGIAIPSERRKGNGKFLELKGANG
                     NNLQRVDAKFPLGKLIVITGVSGSGKSTLINETLYPILSKHAYGSRMTPLEYKSIKGL
                     EEIDKVIEIDQSPIGRTPRSNPATYCGFFTDIRTLFASVPEAKIRGYNAGRFSFNVKS
                     GRCDVCEGGGMRVIEMNFLPDVYVHCEKCNGRRYNRETLEIRYKGKSISDVLDMTVDE
                     AVEFFQAVPYLYRKIKVLQEVGLGYITLGQSAVTLSGGEAQRVKLGTELGKKDTGRTF
                     YILDEPTTGLHFEDIRHLLEVLNKLVDRGNTVLVIEHNLDVIKVADHIIDLGPEGGDG
                     GGKILFEGT"
     gene            complement(2868..3755)
                     /locus_tag="VGQ51_02760"
     CDS             complement(2868..3755)
                     /locus_tag="VGQ51_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEV9035508.1"
                     /translation="MKTVTKWLAAVSGAAAILLFFACNKSNSSNSNPNIPKGQSQVSL
                     YMMDGPMAFTKVLIDIRQVTVEIDTASKQNDADEDDEWDNNFCGRHRTQANSSIIWDT
                     LDIKPGVYNLLDLRNGTDTLLGTGLITTGKILKIKITLGSDNTVYTDSTTFYPLAVFG
                     PSPSFTINVARTNVQDVSNNEFRIWLDFNLSRSIFFWNGEYLLKPFIVVFNDMTEAKV
                     IGKVLPEGAGALVMGVMGTDTIYAIPWWGGQYQFRNVASGSWDFTFKGRNGYQDTTIS
                     NIKVDSMQKVTIPTVTLHK"
     regulatory      3944..4065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4257..5585
                     /locus_tag="VGQ51_02765"
     CDS             4257..5585
                     /locus_tag="VGQ51_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEV9035509.1"
                     /translation="MQATTQLLHSIPVDPLTGAISVPIYQTSTFVQEAPGVNKGYDYS
                     RTGNPTRATLENLVAQLEGGTTAAAFASGLAAIDAVLKLLKTGDEIIAVDDIYGGAFR
                     LFEQVYKRFGITVHYVDSSQPGLVFNALTPRTKLIWIETPTNPTLKITDIRAIANIAA
                     VGRAPRSEGRQRSDGEGRRQSDGEGRKWSDGEERQRSDGEGRKESDGKILLCVDNTFA
                     SPALQQPLALGADIVVHSGTKYLGGHSDLIAGLVVTADAQLGQQIKFLQNAGGAILGP
                     FDSWLLIRGIETLHLRVRQHCANARAIAAFLEQHPAVDKVYYPGLPTHPGHAVARAQS
                     KGFGGIVSFSLKADSQEAATAFVTSTNLFKLAESLGGIKSLVSHPAQMTHKTIPAERR
                     RAAGVADSLIRLSAGLEDTEDLIDDLRQALDRLPAAAPAKTKKIHQTLSI"
     gene            5599..>6238
                     /locus_tag="VGQ51_02770"
     CDS             5599..>6238
                     /locus_tag="VGQ51_02770"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV9035510.1"
                     /translation="METHKQLTIGLFGFGVVGEGLYKVLQQTPSLKATIKRVCIKHPG
                     KKREAPAELFTTDRDVLLNDPEINVIVEVINESGPAYEIVSRALRSGKSVVSASKKMI
                     AEHLTELLQLQKETTASLLYESAACASIPVIRNLEEYYDNDLLHSIKAIVNGSTNFIL
                     TKMFEEKLDFQEALLLAQQLGFAESDPSLDVEGYDAANKWTFLLTHAYGIVEN"
BASE COUNT         1396 a         1693 c         1694 g         1455 t
ORIGIN      
        1 gcgtgccttc gaaaagtatc ttccctcctc cgtcaccacc ttcgggaccc agatcgatga
       61 tatgatcggc gactttgatc acatccaggt tgtgctcgat gacgagaacg gtattgcccc
      121 tgtccaccag cttattcagc acctccaaca aatgccggat gtcttcgaaa tgaagccccg
      181 tcgtaggttc gtcaaggata taaaaggtcc ggccagtatc cttcttgccc agctcggtac
      241 ccagtttcac acgttgcgcc tcgccgccgc tgagcgtgac ggccgactgt cccagggtga
      301 tgtatcccag acccacttcc tgcaggacct tgatcttgcg atacagataa ggcactgcct
      361 ggaagaattc caccgcctcg tcgacggtca tgtcgagcac atccgagatc gatttaccct
      421 tataacggat ctccagcgtt tcgcggttgt agcgccgccc attgcatttc tcgcaatgaa
      481 cgtagacgtc gggcaggaaa ttcatttcta tcacgcgcat cccgccgcct tcacagacat
      541 cgcaacggcc gcttttgacg ttgaaggaga acctgccggc attatacccg cggatcttcg
      601 cttccggtac cgaggcaaaa agcgtccgga tatccgtgaa gaacccgcaa taggtagccg
      661 ggttgctgcg tggcgtcctg ccgatcggcg attggtctat ctcgatgacc ttatcgatct
      721 cttcgagacc cttgatgctt ttatactcaa gtggcgtcat gcgcgatccg taggcatgtt
      781 tgctgaggat gggatacagc gtctcgttga tcagggtaga cttgccgctg ccgctgacgc
      841 ccgtaatgac gatcagcttg cccagcggaa attttgcgtc tacgcgttga agattgttgc
      901 cgttggctcc tttcagctcg aggaacttac cgttcccttt ccgcctttcg gagggtatgg
      961 caatgccgcg ccggccattg agataatcgg cggtgagcgt attgagccgg agcaacgcgg
     1021 gcggacgccc ctgtgctact acgcggcctc cgtgaaatcc ggcctgtgga cctatatcaa
     1081 ccaaataatc cgccgccagc atgatatcct tatcatgttc tacgaccagt acgctgttac
     1141 cgatatcgcg aagatttttc aacgcctgga tcagccggtg gttatcgcgc tggtgcagcc
     1201 cgatgctggg ctcatccagg atataagtaa ttccctgaag ctgtgagccg atctgcgtcg
     1261 ccagacggat gcgctgggac tcaccgccgc tcagggtacg ggacggccgg tcgagcgtca
     1321 gataggtaag tccgacatcc gtgaggaacc gcaatctttc gcgtatctcc ttgaggatat
     1381 ccttggcgat gatgttctgt ttgtcgctga gctttttttc aattccggca aaccagtcca
     1441 cgagccggtt gaggttcatg ctgctgagct cggcaatgtt cttttctccc accctgaacc
     1501 agagactatc cctgcgaagc cgtgttccgc cgcaggtgcc gcaggtcttc agctccatga
     1561 actgttcgac ccattcgcgt aacgaatcgg tagtaccgcc cgcgaaccag cgtttcaggg
     1621 cattgacgat cccttcatag tcggaggcat acacttgtcc cgtctccgca ttggcggcat
     1681 cattgaactc cacctcgagc gtcgcttcgc cggtctcatt cccataaagc accaggttca
     1741 gcgctttttt cgacatatct tctataggcg tgtcgagcga gaacttattc tttttggcca
     1801 gcgcctgtac ctggcggaac atgtaggcct cgcgttctcc gcctaacggc gcgatgccac
     1861 cttcgttgat actcttgctg cgatcgggga tgatggcgtc cattccgatc tggtagacgg
     1921 cgccaagtcc tttacaggtg gggcaggccc cataaggcga gttgaaggaa aagctattgg
     1981 ggcttggttc ctcgtagctg atgcccgagt cttcgcacat cagctgtttg ctgtattgga
     2041 tgaccttgtt atcgtcgttg agcaggagga aaagcaggtc ctttcccatc tgaagggcct
     2101 tctggacgct ttgcccgaga cgggccttca tttcgtcggt gacggccagc cgatcgatca
     2161 cgacctcgat gtcgtggatc ttgtaccggt ccacctgcat cttgggaacg aggtctttca
     2221 cctccccgtc gacgcgcacc ttgagataac cctttttgcg gatatcctcg aacagctcgc
     2281 gatagtgacc cttgcgtccg cggaccaatg gcgccaggat cgatattttc ttattcctga
     2341 atttcttaaa gacgttgtcg agtatctctt cctcgctgaa tttggtcatc acttttccgg
     2401 tattgtagga atgcgcgatg ccgatgcggg catacaacag gcgaaggaaa tcgtagatct
     2461 ccgttacggt gccgacggtg ctgcggggat tcttgttcgt agttttttgt tcgatggaga
     2521 tgaccgggct gagtccggtg atcttgtcga catcgggccg ttccatgtct cctatgaact
     2581 gccgcgcgta agcgccaaag ctctccatat aacgacgctg gccttccgcg tagatggtgt
     2641 cgaaggccag tgaagattta ccgctgccgc taatgccggt gatgaccacc agctggttct
     2701 taggaatgcg tatgtcaatg tttttcagat tgtggaccct cgccccggat acttcgatca
     2761 tttcttccat agggggacaa agttactaaa aaatttgggt cggagacaag ctccgaccca
     2821 aagccaattg gacaggtcaa atgattatgg gtcaactatt tatacaacta tttgtgcaaa
     2881 gtgaccgtgg gtatggtgac cttttgcatc gagtctacct tgatattgct gatcgtcgta
     2941 tcctggtagc cgttccggcc cttgaaggtg aagtcccagg agcccgaggc cacatttcgg
     3001 aactggtatt gtccgcccca ccacggtatc gcatagatgg tatcggtacc catgactccc
     3061 atcaccaggg cgcctgcgcc ttccggcaac accttgccta tgaccttggc ctccgtcata
     3121 tcattgaaca cgacgatgaa aggcttgagc aggtattctc cgttccagaa gaagatggac
     3181 cgggacaggt tgaagtccag ccagatcctg aactcgttgt tggagacatc ctgcacattg
     3241 gtcctggcca cattgatcgt gaaagacggg ctcgggccaa agaccgccag cggataaaag
     3301 gtcgtgctgt cggtgtagac cgtgttatcg gaacccaggg tgatcttgat cttgagtatc
     3361 ttacccgtgg taataagacc cgtacccaac agggtatccg taccgttgcg cagatccagc
     3421 agattgtaaa cgccgggctt gatatccagc gtgtcccaga tgatgctgct gttagcttgc
     3481 gtgcgatgac ggccgcaaaa gttgttatcc cattcgtcgt cttcgtcggc atcgttctgt
     3541 ttggaagccg tatcgatctc gaccgttacc tggcggatgt cgatcaatac tttcgtgaaa
     3601 gccataggtc cgtccatcat atacagagaa acctgggact gacctttagg gatattcggg
     3661 ttggagttag aggagttgct tttattacag gcaaagaaga gtaggatggc tgcggcgccg
     3721 gagacggccg ccagccattt agttacagtt ttcatgtcgt gaagttttag tgtagattta
     3781 ctccgataga tgtctttcag ttgccaaaaa ccgttccaaa atctcgttat tgatacgtta
     3841 agcacacgac cgccctccgg aacaacctgc cggcagggtg aattccgggc aaaatttggc
     3901 ggatcgaaaa aagatattac ctttgccgca gttcttaaaa catctcttat caagaaaggc
     3961 tgagggaaat ggcccgatga agccttgaca acctgcgttc atttacgttg tgaatgaaag
     4021 taaggtgcca actccatccc gatggaatat cgggacagat aagtcagaat cccggcttga
     4081 cgataatgat aaaacatatt caaagctctt ctgacgaatc agaagagttt ttttttgccc
     4141 ggatgcgaac atccgggggg aggactccga tgattaactt ggtaagtggt gtcgctgaac
     4201 aattttttaa accgggcccc ttcgggcggc cctgaacgtc aaaaaaaatt tgttttatgc
     4261 aagcgacaac acaactgctc cacagcattc ccgtagatcc cctcaccggt gcgatctcgg
     4321 tgcccatcta ccaaacctcc actttcgtgc aggaggcacc tggtgtgaac aaaggctatg
     4381 attattcccg tacgggcaac ccgacgaggg ctacactcga aaacctggta gcgcaactgg
     4441 aagggggcac aacggctgcg gcctttgcca gcggactggc ggccatagac gccgtgctga
     4501 aattattaaa gacgggcgac gagatcatcg cagtggatga catttacggc ggcgccttcc
     4561 ggcttttcga acaggtgtac aagcgtttcg ggatcaccgt ccactatgtt gacagcagcc
     4621 aaccgggatt ggttttcaac gcgctgacgc caagaacgaa attgatctgg atcgagaccc
     4681 caaccaaccc aacgctaaag atcaccgata tccgggcgat agcgaatatt gccgcagtcg
     4741 gacgggcacc caggtcagag gggcgtcagc ggtccgacgg agaggggcgt cggcagtccg
     4801 acggagaggg gcgtaagtgg tccgacggag aggagcgtca gcggtccgac ggagaggggc
     4861 gcaaggagtc cgacgggaaa atcctcctat gtgtggacaa taccttcgct tcacccgccc
     4921 tccagcagcc actcgcatta ggcgccgaca tcgtcgtcca ctcgggtacc aaatacctgg
     4981 gcggacacag cgatctgatc gccgggctgg tggtgacggc cgacgcgcag ctcggtcagc
     5041 agattaagtt cctgcagaat gcaggcggcg ccatcctcgg accattcgac agctggctgc
     5101 tgataagagg cattgaaaca ttgcatcttc gcgtacgaca acattgtgcc aacgcaaggg
     5161 ccatcgcggc tttcctggaa cagcatccgg ccgtagataa agtatattat cccggcttgc
     5221 cgacgcatcc gggtcacgca gtcgccaggg cgcagtcgaa gggattcggg ggtatcgtca
     5281 gcttttcgct gaaagccgat tcacaggagg ctgctaccgc cttcgtgacc tcgacgaacc
     5341 ttttcaagct ggcggagagc ctgggtggga tcaagagcct ggtatctcat ccggcgcaga
     5401 tgacgcataa gacgattccg gcggaacggc gtcgggcagc gggtgtggcc gacagcctga
     5461 tccgtctttc ggcgggtctg gaagatacgg aagacctgat cgacgatctt cgtcaggcgc
     5521 tcgaccggct accggcagcc gcaccggcaa aaacaaaaaa gattcatcaa acattgtcaa
     5581 tataacgagt ataataatat ggaaacgcat aagcagctga cgatcggact tttcggattt
     5641 ggcgttgtag gggaaggact ctataaggtc ctgcagcaga ccccgtctct caaggccacc
     5701 atcaagaggg tatgtatcaa acatccgggc aagaaaaggg aggccccggc ggagctcttt
     5761 accacggacc gcgatgtact gttgaacgat ccggagatca atgtcatcgt cgaggtcatc
     5821 aacgaatccg ggccagccta tgagatcgtt tcgcgggcgc ttcgcagtgg caaatccgtg
     5881 gtcagcgcca gcaagaaaat gatcgccgaa caccttaccg agctgcttca gctgcaaaag
     5941 gagaccaccg cctcgctatt gtacgaatcg gcggcctgtg cttcgatacc ggtgatccgt
     6001 aatctcgagg aatactatga caacgacctg ctgcattcca tcaaggccat cgtcaatggc
     6061 agcacgaatt tcatcctgac gaagatgttc gaggaaaagc tggatttcca ggaagccctg
     6121 ttgttggcac agcagctggg tttcgcggag agcgatccat cgctggatgt ggaaggatat
     6181 gacgccgcca acaaatggac cttcctgctg acgcacgcct atggcatcgt ggagaaca
//
LOCUS       DASYSV010000092         6828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_917289, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000092 DASYSV010000000
VERSION     DASYSV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917289"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VGQ51_02775"
     CDS             <1..589
                     /locus_tag="VGQ51_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9035511.1"
                     /translation="SFKSEIKPFAFMLNNNRQDNFTIKVDAAGLNNTIAAIQKKWTSY
                     AGERPFRYSFLDETFANLYKSDQRFNQVILYLTILAITIGCLGLFGLTAFTVERRTRE
                     IGIRKVVGASAGSIVVLLSKDFIRLVMLAILIAVPIAWYAMDKWLQNFAYRVHISWWI
                     FLLAGALAVLIALVTISFQAVRAALTNPVRALRSE"
     gene            700..1626
                     /gene="rbsK"
                     /locus_tag="VGQ51_02780"
     CDS             700..1626
                     /gene="rbsK"
                     /locus_tag="VGQ51_02780"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEV9035512.1"
                     /translation="MRPSILVAGSSNTDMVVKSPSLPSPGETILGGTFFLFPGGKGAN
                     QAVAAARLGGNVIFLAKTGADIFGAQSVEGFKKEGIDCRYIATDPSAASGVALINVDA
                     KGENCIVVAPGANATLTPEEVLKARDAIRNADIVLTQLEIPLNTVIELVSFAAACDRR
                     IILNPAPACNLPDYLLQNVSILTPNEKEAELLTGVKVTDTAGALTAAQILFAKGVATV
                     IITLGRGGALLYDGREPALIPAPKVEAVDTTAAGDVFNGALAVALSEGMRMTDAVKFA
                     NHAAALSVTKLGAQTSAPRRAETETFLLLNNS"
     gene            1634..3562
                     /locus_tag="VGQ51_02785"
     CDS             1634..3562
                     /locus_tag="VGQ51_02785"
                     /inference="COORDINATES: protein motif:HMM:NF018506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEV9035513.1"
                     /translation="MFIVNDYTTAVLLCIITMLCWGSWANTQKLAGRSWRFELFYWDY
                     VIGILLFSIVFGHTLGTLGSGGRHFIDDLHQASWSNFFSAFIGGIIFNAANILLAAAI
                     AVAGMAVAFPIGIGMALVLGVIINYAADQKGSPLWLFAGVACIILAILLNAAAYRRAS
                     RTTLKGILLPITAGILMSFFYRFVAASMDLDNFAHPAAGKMTPYTAMYVFALGIFISN
                     FFFNTLFMRRPVSGQPVSYKEYFAGPWKTHLVGLLGGLIWGLGNGLNLVASGKAGPAI
                     SYGLGQGATLVAAAWGVFIWKEFSPASAKTRRFIAGMFVFFIAGISMIVYAGSLPGSA
                     LPQQPPVSVIFDSDIGPDYDDVGAITLLHAFADSGEANILATMASNKYEGIAGVLNVF
                     NTYFHRPDIPIGVPKGNGVSLRDNQHWTDTLLAKYPHAIKTNSQAEDAVTLYRKILSQ
                     QPDTSVTIISVGFLTNLAGLLKSNPDNYSDLDGRQLVERKVRRLVSMAGSFPKGREFN
                     IYMDSTSSQYVTDNWPTRIFFSGFEIGVKIKCGLPLIHNDAIQNSPVKDVFRISIPQA
                     AEDSLGRSSWDETAVLVGIKGYWEWYSIHRGSIHVKPDGSDFWEPSEVGRHYYLEERK
                     APSVVQGVIDQLIMHQPK"
     gene            3855..4442
                     /locus_tag="VGQ51_02790"
     CDS             3855..4442
                     /locus_tag="VGQ51_02790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEV9035514.1"
                     /translation="MENPQTYRAVKFDRYGGIDVLHIDNVSMQEPYADQVLVKVRAAG
                     INPGEAAIREGWMDKQWPAHFPSGQGSDFAGVVEKVGPGVKNFAHGDAVIGFTDSRAS
                     QAEYVVAEAGHLVRRPVHVPWEQAGALFVAGTTAYAAVRAVDLKKGDNLVISGAAGGV
                     GCIALQLALLKGARVIGLSQSRASCLDLLKRRDPR"
     gene            4456..4791
                     /locus_tag="VGQ51_02795"
     CDS             4456..4791
                     /locus_tag="VGQ51_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV9035515.1"
                     /translation="MTERILAGTDGKVDAFIDTYGHGYVDLALQLGVHPDRIDTIIDF
                     AAAQKYPIRTEGNAQGATREIMDELAYLIEKGYLEIPIERVYPLAEVRDAYRHLERRH
                     THGKIVLVP"
     gene            complement(4788..5198)
                     /locus_tag="VGQ51_02800"
     CDS             complement(4788..5198)
                     /locus_tag="VGQ51_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV9035516.1"
                     /translation="MEPLPYKVIKSAKQYKEYCAALEEMVMVRRKTQAQQDTIDLLTL
                     LIEKWDATHNTFADADPVELLNYLMKENKMKAVDVAGELEISKSLVSDILHYRRGLSK
                     EIIRKLASRFKVSQELFNRPYKLVLPAREKAGRG"
     gene            5807..6316
                     /gene="msrA"
                     /locus_tag="VGQ51_02805"
     CDS             5807..6316
                     /gene="msrA"
                     /locus_tag="VGQ51_02805"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEV9035517.1"
                     /translation="MATEKAILAGGCFWGVEELFRNLKGVLSTTVGYTGGDVPNATYR
                     NHGTHAEGIEILFDTEKINYRELLEFFFQIHDPTTRNRQGNDMGASYRSAIFYLDEQQ
                     KDIAEHLIADLNAAHIYRSPIVTELAPAGPFWVAEEFHQNYLQKYPNGYTCHYIRPEW
                     ALKGEKASG"
     gene            complement(6370..>6828)
                     /locus_tag="VGQ51_02810"
     CDS             complement(6370..>6828)
                     /locus_tag="VGQ51_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEV9035518.1"
                     /translation="IKEIPEDFYVNYFLLVDKEILKDETRRREIYTNLEMIHPTDTEK
                     EIVFFSGLFQKMIDMLTLPFTREEFDFGNEAYFAEIYNYMEYVSNLKEVRESKVARGS
                     RHSLYVNRTYFGLYSILSDLKARVETSEARVKGLMEGAGVRAGKAESLPM"
BASE COUNT         1533 a         2177 c         1599 g         1519 t
ORIGIN      
        1 ttctttcaag tcggagataa agccgttcgc cttcatgctg aacaacaacc ggcaggacaa
       61 tttcaccatc aaggtcgacg ccgcgggtct gaacaatacc attgccgcga tacaaaagaa
      121 atggacttcg tatgccggcg agcggccctt ccgttactcg ttcctcgacg agacgtttgc
      181 caatctttac aaatcggacc aacgcttcaa ccaggtgatt ctctatctca ccatactggc
      241 gatcaccatc ggctgtctgg gactctttgg cctcaccgcg ttcaccgtcg agagaaggac
      301 cagggagatc ggcatccgga aggtggtggg cgcctccgcc ggcagcatcg tcgtgctgtt
      361 gtcgaaagac ttcatccgcc tggtcatgct ggcgatactc attgccgtcc ccatcgcctg
      421 gtatgccatg gataaatggt tacagaactt tgcctatcgc gttcacatct cgtggtggat
      481 attcctgctg gcgggcgccc tggccgtgct catcgcattg gtaaccatca gctttcaggc
      541 tgtgcgtgcc gcgctgacca atcccgtcag ggccttgcga agcgaataat gagattcgaa
      601 gcgagtaatg agattcgaag cgaataatgt cattcgaagc gaataatgag attcaatgcg
      661 aataatggtc attcgcaaaa attgacgatc tttagctgca tgcgtccatc tatcctcgtc
      721 gccggcagct ccaataccga tatggtcgtc aaaagcccga gtctcccttc accgggcgaa
      781 accatccttg gaggtacctt cttcctgttc cccgggggta aaggcgctaa tcaggccgtc
      841 gcagccgcgc gtctgggcgg caacgttatt ttcctggcaa agacaggcgc cgacatcttc
      901 ggcgcccaat ccgtcgaagg cttcaaaaaa gaaggcatag attgccgtta tatcgccacc
      961 gacccttccg ccgcctcggg cgtggccctc atcaatgtcg acgccaaagg agagaactgt
     1021 atcgtcgtag cccccggcgc caacgcaacg ctaacacccg aagaggtcct caaagcgcgc
     1081 gacgcgatca gaaacgccga catcgtcctg acgcaactcg agatcccttt gaatacggtc
     1141 atcgagctcg tatccttcgc agccgcctgt gaccggcgca tcattctcaa cccggcgcct
     1201 gcctgcaacc tgcccgacta tctccttcaa aacgtctcta tccttacacc caatgaaaaa
     1261 gaagccgagc tgctcacagg cgtcaaagtc accgacaccg ccggcgccct cacggccgca
     1321 cagatccttt ttgccaaagg tgtagcaacg gtgatcatca cgctcggccg gggcggagct
     1381 ctcttatatg atggtcggga acccgcgctt atacccgctc cgaaggtcga ggccgtcgac
     1441 acgaccgccg ccggagatgt gttcaatgga gcgctggccg ttgccctttc cgaagggatg
     1501 cgtatgaccg atgccgtgaa gttcgccaat catgccgccg ccctttccgt cacgaaattg
     1561 ggcgcgcaga cctccgcgcc ccggcgcgcc gaaaccgaga cctttctatt gctaaataac
     1621 agttaactac accatgttca tcgttaatga ctacaccacg gccgtactgc tctgcatcat
     1681 caccatgcta tgctggggca gctgggccaa cacacaaaaa ctggccggaa gatcctggcg
     1741 tttcgagctt ttctattggg attatgtcat cgggatcctg ctgttttcca tcgtttttgg
     1801 acacacgctg ggaacattgg ggtccggggg aagacatttc atcgacgacc tccaccaggc
     1861 cagctggagc aatttcttca gcgccttcat cggcggcatc atcttcaatg ccgccaatat
     1921 tctgctggcc gccgccattg ccgtggcggg catggcggta gcgtttccta tcgggatcgg
     1981 gatggcactg gtgctggggg tgatcatcaa ttatgcggcc gaccagaagg gcagcccgct
     2041 ctggcttttc gccggagtcg catgcatcat ccttgcgatc ctgctgaatg ccgccgccta
     2101 ccggcgcgct tcccgcacca ccctgaaagg catcctcctg ccgatcactg ccggcatcct
     2161 catgtccttc ttttatcgtt tcgtcgccgc ttccatggat ctcgacaact tcgcccatcc
     2221 cgccgcgggg aaaatgacac cgtatacggc gatgtatgtc ttcgcgctcg gcatcttcat
     2281 cagcaacttc ttcttcaata cgctcttcat gcgacgaccg gtcagcggac aacccgtgag
     2341 ctataaggaa tatttcgccg gaccctggaa aacgcatctc gtcggtctgc tcggcggtct
     2401 tatctggggt ctgggcaacg gcctcaatct cgtcgcctcg ggtaaggcag gccccgcgat
     2461 ctcctatggt ctcggacagg gcgccaccct ggtcgcagcc gcctggggcg ttttcatctg
     2521 gaaggaattt tcgccggcct ccgcaaagac acgccgcttt atcgctggca tgttcgtctt
     2581 cttcattgcc ggtataagca tgatcgtcta tgccggcagc ctgcccggct ccgcccttcc
     2641 ccaacaaccg ccggtctccg tcatcttcga cagcgacatc ggtccagact atgacgacgt
     2701 cggcgccatc accctgctgc atgcctttgc cgacagcggc gaggcgaata tcctcgccac
     2761 tatggccagc aacaaatatg aaggtattgc cggcgtcctc aacgtcttca atacgtattt
     2821 ccatcgtccc gatatcccaa tcggcgtccc gaagggcaat ggcgtgagcc tccgtgacaa
     2881 tcagcattgg acggacacgc tcctcgcaaa atatccgcat gccatcaaaa ccaacagcca
     2941 ggcggaagat gcagtcacgc tctaccggaa gatactgtcg caacagcccg ataccagcgt
     3001 caccatcata tcggtggggt tcctcaccaa tctcgcgggg ctcctgaaga gcaaccccga
     3061 caactattcg gatctcgatg gccggcagct ggtcgagcga aaggtgcgac ggctggtatc
     3121 catggccggt agcttcccga aaggccgtga gttcaatatc tatatggata gtacttcctc
     3181 gcagtatgtc accgacaatt ggccgacgcg tatcttcttc agcggcttcg agataggcgt
     3241 gaagatcaaa tgcgggctcc cgctgataca caatgacgct attcaaaaca gtcctgtcaa
     3301 agatgtcttc cgcatctcta ttccccaggc cgccgaggac agtctcgggc gtagcagctg
     3361 ggacgaaacc gccgtcctcg ttgggatcaa aggttactgg gagtggtatt cgatacatcg
     3421 cgggtccatt catgtcaaac ccgacggcag cgacttctgg gagccatccg aggtcggccg
     3481 gcattattat ctcgaagagc ggaaagcgcc gtctgtcgtt cagggtgtga tcgatcaact
     3541 gatcatgcat cagccgaagt gatgtcgccc ccccgcgcgc ccgcctgcgt ccttgccccc
     3601 gcgtccccgc cgcgcccata aagcaccgcc gccgccccgc acctccgcga cgtcgctcag
     3661 cccgcacccg cgtccccgca ccctccgtcc gcgacgccac ccaccccgca cccgcgtccc
     3721 cgcctcgccc ataaagcacc tccgccgccc cgcccgcgac gccacccaac ccgcgcctcc
     3781 cgcccgcgac gccgaccacc ccccgcgtcc ccccgcgggc acgattttct ctgtaatccc
     3841 ccaaagggcc aatcatggaa aatccacaaa cataccgcgc cgtaaaattc gaccgctatg
     3901 gcggcatcga tgtccttcat attgacaacg tcagcatgca agaaccctac gcggatcagg
     3961 tgttggtgaa ggtgcgcgca gccggcatca atccgggtga agcggccata cgtgaaggat
     4021 ggatggacaa acagtggccc gcgcattttc cttccgggca gggaagtgat tttgcaggcg
     4081 tcgtggaaaa agtaggtccc ggggtgaaaa attttgcaca tggcgatgca gtcatcggct
     4141 ttaccgacag ccgtgccagc caggccgaat atgtggtcgc ggaagccggc catctcgtcc
     4201 gtcgtcccgt gcatgtccca tgggagcagg cgggcgcatt attcgtggca ggcaccaccg
     4261 cctatgccgc cgtcagagcg gtagatctga aaaaaggcga caatctcgtc atatccggtg
     4321 ccgccggcgg agtaggttgt attgccctgc agctcgcgct cctgaaaggc gcccgcgtca
     4381 tcgggctgtc ccagtcccgg gcatcatgcc tggatctcct caaaaggcgc gatccccgtt
     4441 agctatggcg aggggatgac cgaaaggatc ttggctggca ccgacggtaa ggtcgatgcc
     4501 ttcattgaca cctatggtca tggctatgtc gacctcgcct tgcaactagg cgtccatccc
     4561 gatcgcatcg acaccatcat cgatttcgcc gccgcccaaa aatatccgat caggacggaa
     4621 ggcaatgccc agggcgcaac ccgcgaaata atggacgagc tggcctatct catcgaaaaa
     4681 ggttatctcg aaattccgat cgagcgcgtc tatccgctcg ccgaagtccg cgacgcctat
     4741 cgtcatttgg agcgccgcca cacccacggc aagatcgtcc tcgtgcccta gcctcgtccc
     4801 gccttttccc gcgccggcaa gaccaatttg taaggcctat tgaacaattc ttgcgaaact
     4861 ttgaatcttg acgccaattt acggatgatc tctttagaca accctcttct gtaatggagg
     4921 atgtccgaga caagactctt gcttatttcc aattcacctg ccacatcaac cgccttcatt
     4981 ttattttctt tcatcaaata attcaacaac tccaccggat cagcgtcagc gaacgtatta
     5041 tgcgttgcat cccacttttc gatcaataag gtcaacagat cgatggtgtc ctgctgtgcc
     5101 tgcgtcttcc tcctgaccat gaccatctcc tccagagcgg cacaatactc tttatattgc
     5161 tttgctgatt taattacttt gtaaggcaat ggttccatgg cgacttgatt aataaacgct
     5221 aatataatac tgcttcttct ctttacttaa ttgactgtac tccgcatgag tgccgatcca
     5281 gcacacaaac aaatgaacct gtttttgtcc aaatacataa tgacagatca tcctgaagtc
     5341 attccctcct atatcaaaaa tcacccggcc acatccattg cccaatagat ccgcagagcc
     5401 gaatgtccca aaatgtcctc cgggctattc caatcaaccc tgctcaaagt cgacaaccac
     5461 aatctcaatg acgacctgct tcgagcattt tcccttgcat aatcttctat ggtctctctc
     5521 ctgatcaagt gcactctcat acagtcagct ttacaaatat acaaatagtt ctcggattgc
     5581 gaactttccc ccaaaaaaaa tcccctcttt ccccggtaac ctattatccc ggaacccgca
     5641 aagttagaca ccccataaaa tcttgaagaa aaatctgcat aatccccggc tgcaccctcc
     5701 tgttccccat ccccctaacg gtaggaaatt ggccccccct ccccatttcc ccaaacaatt
     5761 tcacctccga tttgtatatt atatgaaata acaaaatcag aataccatgg ccacagaaaa
     5821 agcaatccta gccggcggat gtttttgggg agtcgaagag ctgttccgca atcttaaagg
     5881 cgtcctttcg acgaccgtcg gctatacggg cggagatgtg cccaatgcca cctaccggaa
     5941 tcacggcacc catgccgaag gcatcgagat actgttcgat accgaaaaga taaactaccg
     6001 cgaattgctt gaattttttt tccagatcca cgaccctacc acgcgcaacc gtcagggaaa
     6061 tgacatgggc gcctcttacc ggtcggccat tttctatttg gacgaacagc aaaaagacat
     6121 cgccgaacat ctcatcgccg acctcaatgc cgcccacatc tatcgctcgc ccatcgtcac
     6181 cgagctcgcg cccgccggcc ccttctgggt cgccgaagaa ttccatcaga attatcttca
     6241 aaaatacccc aacggctata cctgtcacta tatccgcccc gaatgggcgt tgaaaggtga
     6301 aaaagcatcg ggctaaccgc cttccgcccg cctctcacat cggtagagac tcagtcttcc
     6361 gcccgcctct cacatcggca aagactccgc cttccccgcc cgcaccccgg ccccttccat
     6421 caaccccttt acccgggcct cactcgtctc tacgcgtgcc ttgagatcac tcaatatcga
     6481 atacagccca aaataagttc ggttcacata gagcgaatgc ctcgaccctc tcgccacctt
     6541 cgactccctc acttctttca gattcgacac atattccata tagttataga tctccgcaaa
     6601 atacgcttca tttccaaaat cgaactcttc ccgtgtaaag ggcagggtca gcatgtcgat
     6661 catcttctga aacaatcccg aaaagaacac aatctctttt tccgtatccg tcggatggat
     6721 catctcgaga ttcgtgtaga tctcccgccg ccgcgtctcg tctttcaata tctccttatc
     6781 caccagtaaa aaatagttca cataaaagtc ttccggtatc tccttgat
//
LOCUS       DASYSV010000093         5093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_19420, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000093 DASYSV010000000
VERSION     DASYSV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19420"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2443)
                     /locus_tag="VGQ51_02815"
     CDS             complement(<1..2443)
                     /locus_tag="VGQ51_02815"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HEV9035519.1"
                     /translation="MSGKFAPIPRTLQIVFLLAAGLLWKTGTGQNRAVIGTVLDSISR
                     QPIPGASITTPDSHHGVLTDQKGQFHIVVGYRVRHLVVSATGYRQLTVSARDEGEPLI
                     ILLSKNFTLLQDVVVRSKPGKYRNKNNPAVELIRKVIANKSKNAPEADAYESYSEYEK
                     TRLLLDNVPHVITDNTLLKKYHFLFGNTDTTILPGHTLIPFYIEEVSSQQYRRKHPDT
                     DKKMVLGHKSVDFGEYLDMKGISAGVNRLFEDIDIYDNTIEAFTMQFTSPVADLAPTF
                     YMYFIRDTVVIDSELLVELYFTPRNPEDLLFRGTLYITLDGNYAVRRAELGVSRHINL
                     NYTREFRIDLDFQKDSSTGRWHLAVSDMLALLSPLPKSMGLVGQRIVRFGRFSTNPLP
                     DSLFSGAAIDSSGLAEVRTDSFWTAGRPVPLSITEARTYANTDSLVRMRSYHRLMDYT
                     TAFTAGYKSVGKFDMGPIGSFWTFNPVEGQRLKFGGRTTTRLNNRWFGESYVAYGFKD
                     RRWKYFVSATYAFNHKSIYTYPLHFVQVSWLDDARALGQENAFAVANNFFTSFSHGDN
                     SKWLYNRVLRVTYIRELASHLSWSFGMKYWRQQPTGSLYYIYMNQADQADTVRQLTTG
                     EASVTLRWAPHEQFLQNKSGRYNIVNRYPIITLQYGKGIEGLFGGEFNYDALHLRVYK
                     RCYISPAGYSDVSLDAGWLSGNLPFPLLVIHPGNPSYFYSQGAYNMMNVGEFVSDHYA
                     GLNIDHFFNGFFFNKIPGLKRLRLREVVAGKILYGGLNDQNNPAVNPGQMKFPTTGGV
                     TSTYALGGKPYIEGSV"
     gene            complement(2457..4526)
                     /locus_tag="VGQ51_02820"
     CDS             complement(2457..4526)
                     /locus_tag="VGQ51_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HEV9035520.1"
                     /translation="MKRCMIWFLASVCCTNVWAQDKTLTGARYGEQDKALTNTGESRH
                     VVLEAIDMGDVHWTTGFWADRFRVCKDSMIPHLWRIYHDKQLCHSYENFLIAAGIDTG
                     SHEGPPFHDGDFFKLLEAVAAVYAITKDHSLDSMMDEAIAVIAACQRGDGYLSTPALI
                     EERNHPGKETAFEDRLNFETYNMGHLMTAACVHYRATGKTTLLKVAIRAADYLASFYR
                     RSTPQLARNAICPSHYMGIVELYRTTHNPVYLQLAKNLIDIRGMVNDGTDDNQDRVPF
                     RDQTTAMGHAVRANYLYAGVADAVAETGDDSLYHCLKAIWQDITDRKMYITGGCGALY
                     DGVSPDATSYTPAHIQEVHQAYGRDYQLPNTTAHNETCANIGNMLFNWRMLLLSGDAH
                     YADVLEQALYNSVLSGVSLDGDRYFYTNPLRASRAFPYTLRWSGGRQEYISLSNCCPP
                     NLARTIAEVQDYAYCSNDRGLWVNLYGGNRLDTHLPQGPLQLDEETGYPWDGNVRLTI
                     RQAASSAFSIFLRIPGWCDKASMTVNGKPVAASFHPGTYAEIHRTWKTGDVLTLRLPM
                     RTELIESNPLVEETRGQVAVRRGPIVYCLESPDMHGGESIFRVALPWKTDLRPVPMTI
                     GKSRLMTLEGTAWLLPRGDGRLYQELPSAPSKTIHIRLIPYYAWANRGASDMTVWMDL
                     ALHARNY"
     gene            complement(4523..>5093)
                     /locus_tag="VGQ51_02825"
     CDS             complement(4523..>5093)
                     /locus_tag="VGQ51_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035521.1"
                     /translation="LPSISFRTFDGHPTIAPARLWERVNNTECPQLYPVFPWGIYGIG
                     RPGLDTAINTFKYDTDAVKFRSFAGWKQDNIFAARLGLTDQAAKFTVAKLKDGPRRFP
                     AFWGPAFDWVPDHNWGGSGMIGLQEMLMQTDGKKIYLFPAWPISWDVHFKLYAPYSTT
                     VEATLKHGRLVQLDVTPEQRKKDIINFLP"
BASE COUNT         1124 a         1403 c         1422 g         1144 t
ORIGIN      
        1 ccacgcttcc ctcgatatac ggctttcctc ccagtgcata ggtagaggtc accccgccgg
       61 tggtggggaa cttcatttgc cccggattga ccgcgggatt gttttggtca ttaagtccgc
      121 cgtagaggat tttgcccgcg acgacctcgc gcaaccggag tcgcttcaac ccgggtatct
      181 tattgaagaa aaatccatta aaaaaatgat cgatgttcag accggcatag tgatcgctca
      241 cgaactcgcc cacattcatc atattgtagg cgccctggct atagaaatag gacgggttcc
      301 cgggatggat caccagcagg gggaacggaa gattaccgct gagccaacct gcgtccaggc
      361 ttacatcgga atatcccgcg ggtgagatat agcagcgttt gtagacgcgg agatgcagcg
      421 cgtcgtaatt gaattcgccg ccgaacaaac cctcaatgcc tttgccatac tgcagggtga
      481 tgatgggata tcggttcacg atgttgtaac gcccgctctt gttctgcagg aattgctcat
      541 gcggagccca gcgcagcgta acggatgcct cacctgtggt tagctgtcgc acggtgtcgg
      601 cctggtcggc ctgattcata tagatgtaat aaagcgatcc ggtgggctgc tgccgccagt
      661 acttcatacc gaacgaccag gacaggtggc tggcgagctc acggatatac gtgacccgca
      721 agacccggtt gtagagccat ttcgaattgt cgccatggct gaaggatgtg aagaaattgt
      781 tggctaccgc aaaggcattc tcctgcccca gcgcacgcgc atcgtccaac cagctcacct
      841 gcacaaaatg caaagggtag gtatagatgg atttgtgatt gaaggcgtag gttgcactca
      901 caaagtattt ccaacgccga tctttgaacc catatgcgac ataggactcg ccgaaccatc
      961 ggttattcag acgcgtggtg gtccttccgc cgaatttcaa ccgttgccct tcaacagggt
     1021 tgaaggtcca gaagctgccg ataggcccca tatcgaattt tccgaccgac ttatatcccg
     1081 ccgtaaaggc tgtcgtataa tccatgagcc ggtgataaga ccgcatcctg accaggctgt
     1141 ccgtattggc ataggtgcgg gcctccgtaa tactcagcgg aacgggacga ccggcggtcc
     1201 aaaagctgtc cgttcgcact tccgccagac cgctgctgtc gatggcggcg ccggagaaca
     1261 gactgtccgg cagtggattc gtgctgaacc gaccgaaccg gacgatccgt tggcccacga
     1321 gccccatcga tttggggaga gggctgagca gggcgagcat gtccgaaacg gccaggtgcc
     1381 atcggccggt gctgctgtct ttttgaaagt ccaggtcgat cctgaactcg cgtgtataat
     1441 tcagattgat atgccggctg acgccgagct cggcccgtcg gacggcatag ttgccgtcga
     1501 gcgtgatata gagggtcccg cggaaaagca ggtcttcggg gttgcgggga gtaaagtaca
     1561 attcgacgag cagttcgctg tcgatcacga cggtgtcgcg gatgaaatac atgtagaagg
     1621 tgggagcgag atcggccacc gggctggtga actgcatagt gaacgcctcg atggtattgt
     1681 catagatatc gatgtcttcg aacagacggt tgaccccggc gctgataccc ttcatatcga
     1741 gatattcccc gaaatcgacg cttttatgac cgagcaccat cttcttatcc gtatccggat
     1801 gtttgcgccg atattgctgc gacgacacct cttcgatata gaacgggatc agcgtatgac
     1861 ccggaaggat ggtcgtatcg gtgttcccga aaaggaaatg gtattttttc agcaacgtat
     1921 tgtcggtgat gacatgtggt acgttatcga gaaggagcct cgtcttctcg tattccgaat
     1981 aggattcata ggcgtccgcc tccggagcgt tcttcgattt attggcgatg accttcctga
     2041 tcagctcgac cgccggattg ttcttgttcc ggtattttcc cggtttgctc ctgacgacga
     2101 catcctgtaa gagcgtaaaa tttttcgaga gaaggatgat aagcggctct ccttcatccc
     2161 tggcggagac cgtcaactgc cgatagcccg tggccgaaac gaccagatgc cggacccgat
     2221 agccaactac gatatgaaac tgcccttttt gatcggtgag caccccgtgg tggctatccg
     2281 gcgtcgtgat actggcgccc gggatgggtt gacgcgaaat agaatccaac acggtcccga
     2341 tgacggccct gttctgaccg gtcccggttt tccacaaaag tccggcggcc agcaagaata
     2401 cgatctgtag tgtacgcgga ataggcgcaa attttccaga cacgaaggta aggtaatcaa
     2461 taatttcgcg catgcagcgc cagatccatc cacaccgtca tatcggaagc cccccggttg
     2521 gcccaggcat aataggggat gagccggatg tggatcgttt tggagggcgc ggaggggagt
     2581 tcctggtaga gcctgccgtc gccgcgtggc aacagccacg cggttccttc gagcgtcatc
     2641 agcctgcttt tgccgatggt catcggcacc ggccgcagat cggtcttcca tggcaatgcg
     2701 acgcggaata tcgactcccc cccgtgcata tcgggagatt ccagacaata tacgatgggg
     2761 ccgcgtcgca ccgccacctg gccgcgtgtc tcttctacca gcgggttgct ttcgatgagc
     2821 tccgtacgca tgggcaaccg tagggtgagg acatccccgg tcttccatgt ccgatggatc
     2881 tctgcataag taccggggtg aaacgatgcg gcgaccggct tgccgttcac ggtcatactt
     2941 gccttgtcac accagccggg gatacgcaga aagatcgaaa atgcgctcga tgcagcctgt
     3001 cgtatcgtca agcgcacatt tccgtcccag ggatagccgg tctcttcatc cagttgcaac
     3061 ggaccctgtg gcaaatgcgt atcgagcctg ttgcccccgt acagattgac ccaaagaccg
     3121 cgatcgttgg aacaataggc atagtcctgc acttccgcaa tggtccgcgc gagattgggc
     3181 ggacagcaat tggacagcga gatatattcc tgtctgcccc ccgaccagcg cagggtataa
     3241 ggaaaagcgc gcgaggcgcg tagcggattc gtatagaagt agcgatcccc gtcgagactt
     3301 accccggaca acacgctatt gtaaagcgcc tgttccagca cgtcggcata atgggcgtcg
     3361 ccgctgagca gcagcatccg ccagttgaac agcatattcc cgatgttggc gcaggtttca
     3421 ttatgcgccg tcgtatttgg cagctggtaa tcgcggccat aggcctggtg cacctcctgt
     3481 atatgtgccg gcgtataaga ggtggcgtcg ggcgataccc catcatagag ggctccgcat
     3541 ccaccggtga tgtacatttt cctatcggtg atatcctgcc agatggcttt cagacaatga
     3601 tatagcgaat cgtcgccagt ctcggcgacc gcatcggcga cacccgcata caggtagttg
     3661 gcgcgaacgg catgacccat ggcagtagtc tggtcccgga aaggcacacg atcctggtta
     3721 tcgtccgtgc cgtcattcac catcccgcgg atatcgatca gatttttcgc cagctgcaga
     3781 tataccgggt tatgggtagt ccggtacaat tcgacgatac ccatgtagtg agacgggcag
     3841 atggcatttc gcgccagctg gggcgtgctt ctccggtaga aggaggccag ataatccgcc
     3901 gcccggatag ctaccttcaa cagcgtcgtt tttcccgtgg cgcgataatg aacacaggcc
     3961 gcggtcatga gatgccccat attgtaggtc tcgaaattca accggtcttc gaaagccgtc
     4021 tccttaccag gatgattgcg ttcttcgatg agtgcaggtg tagacaaata tccgtctcct
     4081 ctttggcagg cggcgatgac ggcgatggcc tcgtccatca tcgagtccag gctatggtct
     4141 ttggtgatgg catagacggc cgccacggcc tccagcagct tgaaaaaatc gccgtcatga
     4201 aagggtgggc cttcgtggga cccggtatcg atacctgcgg caatgaggaa attctcgtag
     4261 gaatgacata gctgtttatc atgatagatc cgccagagat gagggatcat gctatccttg
     4321 caaacccgaa aacgatcggc ccagaaaccg gtcgtccagt gaacatcgcc catatcgatg
     4381 gcttcgagca cgacatgcct gctttccccc gtattcgtca gtgccttgtc ctgctcaccg
     4441 taccgcgctc ccgtcagcgt cttgtcctgc gcccatacgt tcgtgcagca aacactcgct
     4501 aagaaccata tcatacatcg cttcatggca aaaaatttat aatgtctttt ttccgttgtt
     4561 ccggtgttac atccagctgt accagccggc cgtgtttcaa tgttgcttcg acggtcgtgg
     4621 agtagggggc gtataactta aaatgcacat cccagctaat gggccaggcg gggaagagat
     4681 aaattttttt tccatcggtc tgcatcagca tttcctgcag cccgatcatt cccgaaccac
     4741 cccaattgtg atcgggaacc cagtcaaaag caggtcccca aaaagccggg aaccgtcgcg
     4801 ggccgtcctt caacttagcg acggtgaatt tcgccgcctg atccgtcagt cccagtctcg
     4861 ccgcgaagat attgtcctgt ttccatccgg cgaacgaccg gaatttcacc gcgtcggtat
     4921 cgtatttgaa ggtattgatg gcggtgtcca ggcccgggcg tccgatacca tagatgcccc
     4981 aggggaacac cgggtaaagc tgcggacatt cggtattgtt gacacgctcc cataaccgcg
     5041 caggggcaat cgtcggatga ccgtcgaagg tccggaaaga gatagacggc agc
//
LOCUS       DASYSV010000094         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_789645, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000094 DASYSV010000000
VERSION     DASYSV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789645"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VGQ51_02830"
     CDS             <1..260
                     /locus_tag="VGQ51_02830"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV9035522.1"
                     /translation="FQDVEDDIKKLVPEGIEGRVAFKGSLSEVVQQFIGGLRAGMGYC
                     GSKDIRTLQQQAQFIRITNAGMKESHAHDIEITREAPNYSR"
     gene            273..1832
                     /gene="lnt"
                     /locus_tag="VGQ51_02835"
     CDS             273..1832
                     /gene="lnt"
                     /locus_tag="VGQ51_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEV9035523.1"
                     /translation="MQKLHPFLLSALSGLLLYAAWPMSPFTFLIFVAFIPLLWLERRG
                     IRRRSFFGWVYLSMLIWNIGVTWWIWNSTGPGAAAAIMANSLLMCLPWLGFRWVRRSC
                     GDAFGYLSLLVFWLSFEYLHLHNWGLSWPWLTLGNVFATHTGWVQWYEYTGTSGGGLW
                     ILAANILLFQLWISRRKRTLLIPTIIVIFLPLGFSALLGNHTPDKPTASIGVVLVQPN
                     IDPYGKLVAGSFEAQLQELIRLSESAIDSNTKLVVWPETAIFSDNGFIEDSLKENYFL
                     NPLWAFLRRHPNINLLTGIESLRIFTYKHSPTALKIPNSKDYYESYNEAIILDSSGPV
                     SFYHKTMLVPGVETLPPFLHFLDSWFEKFGGTTAGYTGQADRTPLATSDKGLVIAPSV
                     CYESIYGEFMAGFVDNGAGLLAVITNDAWWGNTPGHLQLENYARLRAIETRRWVVRSA
                     NTGISCFIDPAGMIVGSGPWEQPAVIRRQVPILQQLTFYTRYGDILSKIILALAAFLL
                     AFTIIKRIRRG"
     gene            1825..2580
                     /locus_tag="VGQ51_02840"
     CDS             1825..2580
                     /locus_tag="VGQ51_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV9035524.1"
                     /translation="MDKTKDLPVMLIHGFAEDSTVWDGIASRLENTCRLLIPDLPGSG
                     SSPLPEGEISIDTLAAHCVGLLEKENLDRCIFIGHSMGGYITLAIAEHYPEKVLAFGL
                     FHSTAYADTEEKKANRRKSIDFIRQYGATPYIRQSTPNLFAAGTREHRPQLVENMILR
                     YSGFSGEALVAFLNAMMQRPDRLSVLERFTVLFIIGEKDQIVPMEQSLKQSHMPPISQ
                     VRVLREAGHMGMLEDPDAGSGAIRSFINFIQQS"
     gene            2577..3332
                     /locus_tag="VGQ51_02845"
     CDS             2577..3332
                     /locus_tag="VGQ51_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV9035525.1"
                     /translation="MNRTIIITGATSGFGEACARKFAAGGDHLIISGRRKDRLESLQR
                     ELGNKVRVLPLVFDVRERKAVFDAVSGIPESWRKIDILINNAGLALGRDYFDQADIDD
                     WETMIDTNVMGLLYMSRAVLPLMIPSGRGHIINLGSVAGKDVYEKGNAYCGSKFAVDA
                     LSRSMRIDLLRHGIKVTAIHPGAAETEFSIVRFKGDETQAKKIYQGFTPLSADDVADV
                     IHYCASLPPHVCINDLVLTPIAQADGIYFHKKP"
     gene            complement(3348..>3828)
                     /locus_tag="VGQ51_02850"
     CDS             complement(3348..>3828)
                     /locus_tag="VGQ51_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035526.1"
                     /translation="FVNLMTGKLGYSRSRTLSLSLIDYQLSETHSSEFSIALGYRKRN
                     VGLPFGWKVPGNGKTPPNGPPGSINAQKGSNDLSLNLQIDIRDDAMSSTYLDQGTSLP
                     TGGQRVIRIEPSIDYVLNNRINLKFYFDQTRTTPKISTTPPIVTTKGGIQIRIALAP"
BASE COUNT          874 a         1111 c          981 g          862 t
ORIGIN      
        1 acttccagga cgtcgaagac gatatcaaaa aactggtgcc ggaagggatc gagggccggg
       61 tcgcattcaa aggcagtctc tcggaagtgg tacagcaatt catcggaggt ctgcgggcgg
      121 gcatgggata ttgcggctca aaggatatcc ggacccttca acaacaagct caattcatcc
      181 gaatcaccaa cgccggcatg aaagaaagtc atgcacatga tatagagata acgagggaag
      241 cgccgaatta cagccggtaa aacccatcca ccttgcaaaa actacatccc tttcttcttt
      301 cggcgctcag cggtctgtta ttgtatgcag catggccgat gtcgccattc accttcctga
      361 tcttcgtcgc tttcatcccg ctgttatggc tggagcgaag aggcatacgc cggcgctcct
      421 ttttcggctg ggtatatctt tctatgctaa tctggaatat cggtgtcacc tggtggatct
      481 ggaactctac cggacccgga gccgcggcgg caatcatggc caacagtcta ctgatgtgtc
      541 ttccctggct gggttttcgc tgggtcaggc gtagttgcgg agacgcgttc ggctatctgt
      601 ccctgctggt cttctggctc agctttgaat acctccacct gcacaactgg gggctcagct
      661 ggccctggct gactctcggc aacgtctttg ccacccacac aggctgggtc caatggtatg
      721 aatatacggg taccagcggc ggcggcctct ggatccttgc agcgaacatc ctcctgttcc
      781 aattatggat ctcccggcgc aaacgcacac tgcttatccc gaccatcatc gtcatcttcc
      841 tgccgttagg tttttcggcc ctcctgggca atcatactcc ggataaaccc accgcctcga
      901 tcggagtagt cctcgtacag ccgaacatcg acccgtacgg caagctggtg gcgggcagtt
      961 tcgaagccca attgcaagag ctcatccggc tcagcgagtc ggccatcgac agcaatacca
     1021 aactcgtcgt ctggcccgag acggccatct ttagcgataa cggattcata gaagacagtt
     1081 taaaggaaaa ctatttcctc aacccactat gggcatttct ccgccgccat ccgaacatca
     1141 acctgctcac cggtatcgaa agcctgcgga tattcaccta caaacactcc cctaccgccc
     1201 tgaagatccc caattcgaaa gattattacg aaagttacaa cgaggcgatc atactagaca
     1261 gctcgggacc cgtgtccttc taccataaga ccatgctcgt gcccggggtc gaaacacttc
     1321 ctccctttct ccatttcctc gacagctggt tcgaaaaatt cggagggact accgcgggtt
     1381 acaccgggca agcagaccgt accccactcg ccacgtccga caaaggcctt gtcatcgctc
     1441 cttccgtatg ctatgaaagc atttatggag agttcatggc cgggttcgtc gacaacgggg
     1501 ccgggttgct ggccgtcatt accaacgacg cctggtgggg caataccccc ggtcatctgc
     1561 agctggaaaa ctatgcccgt ctgcgcgcca tcgagacccg gcggtgggtc gtacggagcg
     1621 ccaataccgg catttcgtgt tttatagacc cggcaggcat gatcgtggga tccggtccct
     1681 gggagcaacc cgccgtcatc cgccggcagg taccaatttt gcagcagctg actttctata
     1741 cccgctatgg agacatcctt tcgaaaataa tattggcctt agccgcattc ctcctcgcct
     1801 ttaccattat caaaaggata cgccgtggat aagacaaaag acttgccggt aatgctcata
     1861 catggcttcg ccgaagacag cactgtttgg gacggcatcg catcccgtct cgaaaatacc
     1921 tgtcgcctgc tgatcccgga cctccccggc agcggcagct cgccccttcc cgagggagag
     1981 atctccatcg ataccctggc cgcccattgc gtaggtctgc tggaaaaaga gaacctcgat
     2041 cggtgcattt ttatcggcca cagcatgggt ggctatatca ctctcgcgat cgcggagcat
     2101 tatcccgaaa aagtccttgc cttcggtctt tttcattcga cggcctatgc cgacaccgaa
     2161 gaaaaaaagg ccaacagacg taaaagcatc gacttcattc gtcaatatgg cgccaccccc
     2221 tacatccgcc agtcaacccc caacttattt gccgcgggga cgcgggagca tcgcccccag
     2281 ttggtagaaa acatgatcct gcgttattcc ggtttctccg gcgaagccct cgtggccttt
     2341 ctgaatgcaa tgatgcaacg tcccgaccgg ctgtccgtac tggaacggtt taccgtactg
     2401 tttatcatcg gggaaaaaga tcagatcgta ccgatggagc aatccttaaa acaatcgcac
     2461 atgcctccga tatcgcaggt gcgggtgctg cgggaggccg gtcatatggg catgctcgaa
     2521 gacccggacg ccggctcggg cgccatccga tcatttataa acttcatcca acagtcatga
     2581 acaggaccat catcattaca ggtgcaactt ccggtttcgg cgaagcatgc gcccgcaaat
     2641 tcgccgcagg aggcgatcat ctcattatct ccgggcggag aaaggataga cttgagtctt
     2701 tgcaacgcga actcgggaac aaggtccggg tattgccgct ggtattcgac gtccgtgaaa
     2761 gaaaggctgt attcgacgcc gtatccggca tccccgaatc atggcgaaag atcgatatcc
     2821 tcatcaataa cgcaggtctc gcgctgggaa gggattattt cgaccaagcc gatatcgacg
     2881 attgggagac aatgatagat acgaatgtaa tgggcctgct ttatatgtcc agggcggtct
     2941 tgcccctgat gatcccctca ggccgtgggc atattatcaa cctcggcagc gtagccggca
     3001 aagatgtata tgagaaaggg aatgcctact gcggcagcaa attcgcggtg gacgccctca
     3061 gcagatcgat gcgtatcgat ctgctgcgac acgggatcaa agttaccgcc atccaccccg
     3121 gcgccgccga gacggagttt tccattgtcc gattcaaagg cgacgaaaca caggccaaaa
     3181 aaatatatca ggggttcact ccactgagtg cggatgatgt agcggatgtc atacattatt
     3241 gcgcctcgct tccgccgcat gtctgcatca acgatctggt ccttaccccg atcgcccagg
     3301 ccgacgggat ttattttcac aagaaaccct gagggtggca ggggaaatta cggcgccagt
     3361 gcgatcctta tctgaatccc ccccttcgtc gtcacgatgg gcggcgtggt cgatatcttc
     3421 ggtgtggtcc gcgtctggtc gaaatagaat ttaagattga tccggttgtt caatacgtaa
     3481 tcgatggacg gctcgatcct tatcacccgc tgtcctccgg ttggcaacga tgtcccctga
     3541 tcgagatagg tgctggacat ggcgtcatcc cgaatatcta tctgcaggtt gaggctgaga
     3601 tcattggatc ccttctgcgc attgatgctc ccgggcggcc cgttgggtgg agtcttacca
     3661 ttacccggga ccttccagcc gaacggcaac cccacattcc gcttccgata gcccagtgca
     3721 atggaaaatt cggaagaatg cgtctcgctc agctggtaat cgataagact cagactgagg
     3781 gtccggcttc ggctatatcc gagcttaccc gtcatcagat tgacgaac
//
LOCUS       DASYSV010000095        15093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_145659, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000095 DASYSV010000000
VERSION     DASYSV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 15093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15093
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145659"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..2047)
                     /locus_tag="VGQ51_02855"
     CDS             complement(26..2047)
                     /locus_tag="VGQ51_02855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEV9035527.1"
                     /translation="MKKFLYLSVVVSLISACNNPPVEGTRSFVDVAGLDSSIKPGDNF
                     FRLVNGRWYDTVKIADDQTGIGSYSFLNIPQKQLLQHILDSVSGTSHAPGSIDQQVGD
                     FYASGMDTTTINRRGYEPVKPILARIDAVSDEQGLVRLAASESKEGNGSIIGLGIAPD
                     NKNSGINIAHLGQTGIGLPERDYYFKTDSSTLRIQHAYLKYIGTLFELSGSDPATAAH
                     QAEIVYGIEKQLAESHKTNIDLRNVQANYHKLAVASIDKTQPKLHWPLVLASLDAKTD
                     SVDMSQPAYYDRLNGLLISVPVADWKIYLKAATLRNYADALSKPFVDASFAFTKVLTG
                     QDVQKPRGKIMTNQVDGLLGMALGQLYVKRYFNEEAKKRVLALVDNLQKAFENRIKRL
                     DWMSDSTKKKAIEKLYAITKKIGYPDKWRDYSKVQIDRSKYFENILSLQQNDYRYQLA
                     KLNKPVDRSEWGTTPSTVTAYYNPYYNEIVFPAGILQYPYFDFAADDAINYGGIGMVI
                     GHEMTHAFDDQGAQFDKEGNVKNWWTKEDYEKFKERTRRVVDLYNSFTVLDSVHVKGA
                     LTLGENTADNGGIQIAYDAFKMTPEGHDTTRIDGFTPDQRFYLSIGRIWRVKTRDAFL
                     RMYVNTNPHSPAMWRVNGPIMSCAPFYKAFNVQPGDKNYLPEDQRIKIW"
     gene            complement(2082..3374)
                     /locus_tag="VGQ51_02860"
     CDS             complement(2082..3374)
                     /locus_tag="VGQ51_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEV9035528.1"
                     /translation="MLKCIVLIILMSAWGSVYGQVDSLKTDSLRVKLFKGKAPENDTA
                     GQYDFGDLWKAVFHPNRQGGAVHSSSGITVVPNISANPTIGAQIGIKAVAGRRLGNDP
                     KTLFSTGATSASITTKGIIYFYLTHNLFTPGNKFNFQGNLVVAKTVTPDFGLGIGAPF
                     TGGTASDSVLANPTHHAYAIHSFYYNFREKIYKNVAKNLFIGAGVSFEIRRGIRRSDT
                     TENATPSSVYDNRHGMPHDHYSYNGLLFNVEYITRDNPNRAYKGLYTDVGLRINQTWL
                     GSTKNAVQVTTDIRKYIGLSAAVPELVLALWNWGSYLVDGTIPYFELPGTGRDGSFRS
                     GRGYMAQYFKGTEYNDTEAELRFPILRNQLVSGVAFGNLQTCNDEHGTKIFQVFQPGY
                     GGGLRILFNKLTRTNLALDYAFGRFGSKGFFLNLNEAF"
     gene            3583..6024
                     /locus_tag="VGQ51_02865"
     CDS             3583..6024
                     /locus_tag="VGQ51_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035529.1"
                     /translation="MQRNKVNSILNISGLAIAIACVIMIAMYITDELSYDRIFANADH
                     LFQVNMTIRDNGVEGTTGGNTAPAVGPTLKEMYPEVASFTRVYRPGDVVVRYDEPGKQ
                     ETFYSEKNTLGVDSNFLEMFDYKITEGNAATCLQKTNSVVITQSTAKKYFADKNAIGK
                     ILLFDADKKPFVVTAVVKDIPSNSSFRFDMLTPISAHPVVKKRSWNWGWLQVNTWIRL
                     KNSSPVDAASVARLQAKFPEMVKERAFNKDYGQSYDEFIRKGGGLSYSLMPFTSVHLH
                     AIPMGVPARLTTLGNIKYIYIFSIVAFFIVILACVNFMNLSTAQSVTRAKEVGIRKVM
                     GSVKTQLIRQFLMEAMLYSLIATIAGVVLTALLLDPFNAIAGKTLAFDSIFSNKLWLL
                     IPALTLVTAFLAGLYPAFYLTSFNPVEVLKGIRLFKANIGNLLIRNGLVIIQFTIAVV
                     LIICTLIVFQQMKFEQNKDLGLNKENVLVVENTKRLGNNEESFRQELAKQPGILSASI
                     SSGIPTKGSFGDGYVPEPAGGDKPLLSDIGLSSFMVDDAFVPTYRLQMLKGRNFSKTF
                     NDSASVIINETAAQKIGWKDPVGKYLAYPGNAQRFKVIGVVKDFNIESLHEIVEPFAL
                     FHNSSHTYGLFSSYVSVRLQPGTISRQIGSIKTAWQNFAPATPFDFSFLDSDFDALYR
                     SEQQMGAVFRIFTFLSIFVACLGLFGLSIFAAQRRKKEIGVRKVLGASVGNVVRLLSA
                     DFIKLVAVAALIAFPIAWLAMNKWLADFAYRINISWWIFLISGLIAIAIALMTVSFQA
                     IRSATSNPVNSLRSE"
     gene            6026..6793
                     /locus_tag="VGQ51_02870"
     CDS             6026..6793
                     /locus_tag="VGQ51_02870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV9035530.1"
                     /translation="MKPYTITLLLCMAFIRAHAQQPRNLILSHGAILRGDSTKKTIAL
                     VFSGDEFGDGLPAIIKALHKHHVTGSFFFTGRFYRNPGYTSSIQQLYRQHNYLGPHSN
                     GHLLYADWTHRDSTLVTEDSFRTDLQKNLAAMLALGIDTSGPHYFIPPYEWWNDTIAA
                     WCHTIGWHLINFTPGIRTNADYTYPEMGRSYLSSEAILRTLKKYRPSGLNGAIILIHA
                     GTDPRRTDKLYNRLDQLLTQLQSDGYTCLPISKFFSD"
     gene            6839..8125
                     /locus_tag="VGQ51_02875"
     CDS             6839..8125
                     /locus_tag="VGQ51_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV9035531.1"
                     /translation="MKSMTLAGLGLPFAQKSDPRPATLADLKDLISQYGEIAPATLAE
                     NEEFWTGIRAQYNLKPDYANLENGYYNIQPTPVLEAFLRHVKDVNYEGAFYMRTVQFD
                     RKKAIAARLAALGGCTTEELVITRNTTESLDMIIAGYPWKPGDEAIVAEQDYPAMLAM
                     FRQVEKRHGIIVKRVSVPNHPASDADVISVYEQAITPRTRLMMVCHMINITGQVLPVR
                     KISDMAHSHGVEVMIDAAHTYAHIRHTIPELGGDYYAASLHKWLSAPLGAGILYVKKE
                     HQAKIWPLFAEPDGSAEGIARLNHTGTLPVHTDLAIGDALDYYTTLGAERKEARLRYL
                     QQYWTSKIRDVPGIVVNTPADPTRGCAIANVGIKNIAPGDMAARLLQQYRIYTVAIDY
                     PAANVRGCRITPNVYTLTTELDRLVAALRGMASASA"
     gene            8161..10179
                     /locus_tag="VGQ51_02880"
     CDS             8161..10179
                     /locus_tag="VGQ51_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEV9035532.1"
                     /translation="MNWLTLLLFFQTLGHPTPPPHRHPTPPPHLHPSPTPHRHPTPPP
                     DSPFAILTRKQEALHHLPVGAIRPEGWLKDQILSNLLGFTGHLDDLAPDLLIRDDIFG
                     ADRLTKKIKKKDVGALAGGDTSAQIQYLWWNSETQGNWRDGFIRSAILTGDPAAIKRA
                     RIYVRRILATQDPDGYLGIYDEDLRYHFHTENGELWAKTTLLRGLLAWYEYSRDPAVL
                     KAVERAVADVICNYPAGHSHPFYSTTAYVGGLSHGLMFTDILEELFRITGRTSWLDYA
                     VFLYKDFSTQHLNEDAQYSRIIDPAYRLQGHGVHTYEHLRAVAAAAWASGNPSLLSAL
                     KIYLTRISNELMPSGGPAGDEWIGNRTGDATTTGYEYCSLQELMDGYIDLMQKTGSAS
                     YGDRIERLFFNAAQGARDPQASCIAYLKTDNSYQMTGGLNGDTTAHQIRYKYSPVHQD
                     AAVCCVPNAGRIAPYFVDGMWMIGSTGPVATLLGPCTASITWKAQPLKISESTGYPND
                     FTFDFRITAAHPTTFTLSIRKPAWADRFTLNAPYVEKDGYIRVTKTWKTGESIRLRLL
                     PDVKTANDRKGSTFFTYGPLVLAHPISAAGTITKRYPLPGFYDYQYRPDSLTLYHYGG
                     EKISRSPDPYNGFGAIRFKTSLYTDHASRKDSVSLVPMGGTILRQVTF"
     gene            10219..10953
                     /locus_tag="VGQ51_02885"
     CDS             10219..10953
                     /locus_tag="VGQ51_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEV9035533.1"
                     /translation="MPEGPSIVILKEQTQKFSGRKILEVDGNTKVDLTGVAGSKITAF
                     ASWGKHFLIVLPKRTIRIHFMLFGSYSIDEQTKPTPRLHLKFKTGDLYFYACSVKVLP
                     GDIDSQYDWSADVMSDTWDPAKARKKLKTCPEELAADALLDQDIFAGVGNIIKNEVLF
                     RIRVHPESRIGALPARKLTQLISEARIYSFDFLKWKKAFQLRKHWLAHAKTICPRDHI
                     PFVKVKHLGKRKRRAFFCTKCQRLYT"
     gene            complement(10950..12032)
                     /locus_tag="VGQ51_02890"
     CDS             complement(10950..12032)
                     /locus_tag="VGQ51_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEV9035534.1"
                     /translation="MESGTTLALSHKEFLRVDRDHEKAASVAHLRYVRDCQPGILRVK
                     KGKGFRYLFNNKVVGDPGQLERIRKLVIPPGWTQVWICADEKGHIQATGLDLRRRKQY
                     RYHPLWNSLRNETKFHRLYEFGKALPLLRGRVEEDLRAKGLTQDTVLATVVSLMDRTF
                     MRVGNNEYEKANGSYGLTTLKNKHVSISGDKISFSFTGKKGIHHEVTLRDRRLARIVA
                     QCHDIPGKELFEYYGEDGTRHPVDSGMVNTYIREATGEDFSAKDFRTWAGSLHALQEF
                     IRVGEAGTDAATKRNINVVLDSVSSRLGNTRTVCKKYYVHPGLIRLYEEKKLANYLKE
                     EVAEDHAGLTAEEKVLMKILKKFIAS"
     gene            12138..12878
                     /locus_tag="VGQ51_02895"
     CDS             12138..12878
                     /locus_tag="VGQ51_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035535.1"
                     /translation="MNLFKSGSSLLFTGAVILLTSCGSGGNDKTTTADSTAAADSAAK
                     AKAASTIVTTPTNIVEVTHKVANYEKWLAAYEAHDSARLAAGLHNYVIGRGLEDSNMV
                     LVALKCDDTAKAKAFAKDPGLKAAMKKGGVLGTPTISISTETWQDTVTLAPNTIRSVA
                     TFTVKDWDAFVKQFESDKQERLDNGIMDRVVGHDLDDNKKVSIVTALSDSAKATAYHK
                     SDALKKRMEAAGVVGEPARFLFRIVKMN"
     gene            complement(13152..13997)
                     /locus_tag="VGQ51_02900"
     CDS             complement(13152..13997)
                     /locus_tag="VGQ51_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035536.1"
                     /translation="MYVLFGAVIFGGVWVNPLSVSAQHSCADMHSGEYYFYPRNTPQQ
                     YHVVVSGDMEKVVNLNKRKGASYYDSSVYKIEWKGDCDYTLKYQQGSGLGEDDLKFLA
                     KHKVAYHVGGIGPDYFVFSSYIDRASPKNLLANDTMWMHPQTHTEENMVFGITDPGRI
                     RKIHFSDTSRMALLYIYRPGKLKLSFSPLLIYYNDLPMVVLKNKTAAVVAIFKEGAFT
                     LRSRVSETKVEGDLPMEIKFGNSYFVRADMIWGLYKTGNTKLEFTVMEPKEGKQDFDA
                     IYKVE"
     gene            14041..>15093
                     /locus_tag="VGQ51_02905"
     CDS             14041..>15093
                     /locus_tag="VGQ51_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9035537.1"
                     /translation="MKNILSLCLMLGILSTTIAQPLHKKYDLLITNANVVDVPTGKLL
                     KNRLLAISGDTIRAVDDTRKAATYKADKVIDANGKYIIPGLWDMHIHLRGGDSTIEAN
                     KKLLPLFLAYGVTTVRECGGDITPSVMRWRAETANGELAGPRIFTSGPKLDGPHAVWA
                     GSIPVVTPQDVTHALDSLQEIHSDFVKIYDSKISGDAYLEIIRQAKKRGMKVTGHMPY
                     TVELKEAAALGMDGSEHMYYVFKACSSKEDSITNLVREREHTARPMGLFGALPALYAT
                     FDSVKAAQLFRYLAAKHFTITPTLFISKTLAGIKDTDHSKDSLLNYIDPKIQATYAGR
                     IRGANRQSDQARQFTEK"
BASE COUNT         3711 a         4500 c         3427 g         3455 t
ORIGIN      
        1 attgtttttt caatgcttta ctatctcacc agatctttat cctctgatcc tccggcagat
       61 aattcttatc ccccggctgc acattgaacg ccttataaaa tggtgcacaa ctcatgatcg
      121 gcccattgac acgccacatg gccggtgaat gcgggttggt attgacatac atccgcaaaa
      181 acgcatctct cgtcttcacc ctccatatcc tcccgatcga taaataaaat cgttgatccg
      241 gcgtaaaccc atctatcctg gtcgtatcat gcccttcggg agtcatcttg aaggcatcat
      301 acgcgatctg aatacctcca ttatctgcgg tattctcccc caacgtcagc gctcccttca
      361 catgcacgct atccaggacg gtgaaactat tgtacaagtc cacgacccgg cgagtccttt
      421 ccttgaattt ctcataatcc tccttcgtcc accaattctt tacatttcct tccttatcga
      481 attgcgcgcc ctgatcgtcg aaggcatgcg tcatctcatg accgatgacc atcccgatcc
      541 cgccgtagtt gatggcgtcg tctgccgcaa aatcgaaata gggatattgc agaatgccgg
      601 caggaaaaac gatctcgtta taatacgggt tataatacgc ggtgaccgta gacggcgtcg
      661 taccccattc cgatctgtca acaggcttat tcagcttggc cagctggtat cgatagtcgt
      721 tttgttggag cgaaagaata ttttcgaagt atttcgagcg gtcgatctgt actttgctgt
      781 aatcgcgcca cttatccggg taaccgatct ttttggtgat cgcatacaac ttttcgatcg
      841 cctttttctt cgtgctgtcg ctcatccaat cgagacgttt gatcctattc tcgaatgctt
      901 tctgcaggtt gtccacgagc gcgagtaccc gctttttggc ttcctcgttg aaatatcttt
      961 ttacatatag ttgcccaagc gccattccca aaagaccatc aacctgattg gtcatgatct
     1021 tcccgcgggg cttctgtacg tcctgtccgg tcaatacttt agtaaaggca aacgacgcgt
     1081 ccacgaaagg tttgctcagc gcatcggcat aattgcggag cgtagccgcc ttcaaatata
     1141 ttttccaatc ggccacaggc accgagatca gcagtccatt gaggcgatca taataggcag
     1201 gctggctcat atcgacagaa tcggtcttcg catccaggct ggcaagcacc aggggccaat
     1261 gtaacttagg ctgtgtcttg tcgatcgacg ctaccgctaa cttatgatag ttggcctgaa
     1321 cattcctcag atcgatattc gtcttatggg attctgccag ctgcttttcg atgccataaa
     1381 cgatctccgc ctgatgcgca gcagtagcag gatcgctccc gctcaattcg aataaggtgc
     1441 cgatatattt cagataggca tgctgaatac gtagcgtaga cgagtccgtt ttaaaataat
     1501 aatcccgctc gggaaggccg atacctgtct ggcccaaatg cgcgatattg atgccgctgt
     1561 ttttgttgtc gggcgcgatc cccaaaccaa tgattgaccc attgccctcc ttcgactcgc
     1621 tagcagccag ccggaccagg ccctgctcat cggaaacagc atcgattcgc gcgagtatcg
     1681 gtttcaccgg ctcatatccc ctccggttga tggtagtggt gtccataccg gaagcgtaga
     1741 agtcccccac ttgctgatcg atactgcccg gcgcatgcga agtccccgag acgctatcca
     1801 ggatgtgttg cagcaactgc ttttgcggaa tgttcaaaaa gctgtaagag ccgatccccg
     1861 tctgatcgtc cgcgatcttc acggtatcgt accaccgccc gttgaccaaa cgaaaaaaat
     1921 tatcaccggg ttttatcgaa gagtcgagtc ccgccacatc gacaaaagat ctcgtaccct
     1981 caaccggcgg gttattacat gcggaaatca acgatacgac gacagacaga taaaggaatt
     2041 tcttcatacg cagaggtttc actaaaggta atacaccgtc ctcaaaacgc ctcattcaga
     2101 ttcaaaaaga accccttact cccaaatcgg ccaaaagcat aatccagcgc cagattcgta
     2161 cgcgtcaatt tgttgaacaa tatcctcagc cctcctccat aacccggctg aaagacctgg
     2221 aatatcttcg ttccatgctc gtcgttacaa gtctgtagat tcccaaatgc caccccgctc
     2281 accaactgat tacgaaggat agggaaccgc agctctgcct ccgtatcatt gtactccgtg
     2341 cccttgaagt attgagccat atatcccctg ccgcttcgga aggatccatc cctccccgta
     2401 cccggtaatt cgaaataggg tatcgtacca tcgacaagat aggaacccca gttccataat
     2461 gccaatacta attccggcac agcggcagac aagccgatgt atttccggat gtccgtcgtt
     2521 acctgcaccg cattctttgt gctcccgagc catgtctgat tgatcctgag acctacatcg
     2581 gtataaagtc ccttgtaggc ccggttcgga ttgtcacggg taatatattc cacattgaaa
     2641 aggagcccgt tgtaagagta gtggtcatgc ggcatcccat gcctgttgtc ataaacgctg
     2701 gaaggcgtgg cattctcagt agtatccgat cgccggatac cccttctgat ctcgaaagat
     2761 acgccggcgc cgataaaaag gttcttcgca acattcttat atatcttttc cctgaaattg
     2821 taataaaagg aatgaatggc ataggcatga tgcgtaggat ttgccaacac ggaatcgctc
     2881 gccgttccgc cggtaaaggg cgccccgatc cccaatccaa aatcaggggt gaccgtcttt
     2941 gctacgacca ggttaccctg gaaattaaat ttattcccgg gggtgaaaag attatgcgtc
     3001 agatagaaat agataatgcc ctttgtcgtg atagaagccg aggtcgcacc ggtcgaaaag
     3061 agcgtctttg gatcattgcc aagccgcctg cccgccaccg ctttaatacc tatctgtgcg
     3121 ccgatggtag gattggccga tatattcgga accaccgtta ttcctgagct ggaatgcact
     3181 gccccgcctt gccggtttgg atgaaagacc gccttccaca gatccccaaa gtcatactga
     3241 cccgcagtgt cgttctcggg cgcctttccc ttgaatagct tcacccgcaa actatccgtt
     3301 ttaagagagt ccacctgacc atacaccgat ccccaggcgc tcataaggat gatcaatact
     3361 atacatttca gcaagacccg acgtttgtca ttcctcactc aaaataccgt accaatgtcc
     3421 tcctcctgtc ttataaatga acatccactg tcgcgaaacc gaactgatcg ataccctatt
     3481 acaaccttaa acacctgaac atcaagccct tctatttttg gcataggctt agtggccgct
     3541 ggttctatgt ttcggaacta tctcaaaaca gccctgcgca acatgcagcg caacaaggta
     3601 aactccatcc tgaatatttc cgggcttgcc attgcgattg cctgcgtgat catgatcgcg
     3661 atgtacataa cggatgaatt gagctacgat cgcatcttcg ccaatgccga ccacctgttc
     3721 caggtaaata tgacgatcag ggacaacggt gtcgaaggca ccaccggagg taacacggct
     3781 cccgcggtcg gaccaacatt gaaggagatg taccccgaag ttgcgtcgtt cacaagagta
     3841 taccgtccgg gtgacgtggt agtgcgatac gacgaaccgg ggaaacagga aactttctac
     3901 tcggaaaaaa acaccctggg cgtcgattcc aatttcctgg agatgttcga ctacaagata
     3961 acagaaggca atgccgcaac ctgcctccaa aaaacgaact cggtggtcat cacccaatcg
     4021 acggcaaaaa aatatttcgc cgacaagaat gccatcggaa agatcttatt gttcgacgcg
     4081 gataaaaaac ccttcgtcgt aaccgccgtg gtgaaagaca tcccttccaa ttcttcgttc
     4141 cggtttgaca tgcttacccc catctctgca catcccgtgg tgaaaaaacg tagttggaac
     4201 tggggctggt tacaggtgaa cacctggatc cggttaaaaa acagctctcc cgtcgatgct
     4261 gcgtctgtcg cacggcttca ggcgaagttt ccggaaatgg taaaagaacg cgcattcaat
     4321 aaggattatg gtcagtcata tgacgagttc ataagaaaag gtggtgggct cagctacagc
     4381 ctgatgcctt ttacgtcggt gcacctgcac gcgataccga tgggagtgcc cgcgcgtctg
     4441 accacgctgg ggaatataaa atatatctac atcttctcga tcgtcgcctt tttcatcgtc
     4501 attcttgcct gcgtaaattt catgaatcta tccaccgccc aatccgttac aagggcaaag
     4561 gaagtcggca ttcgcaaggt gatgggttcg gtaaaaacgc aattgatacg acagttcctg
     4621 atggaagcga tgctgtatag ccttattgca acgatcgccg gcgtcgtgct gacggccttg
     4681 cttctggacc ctttcaatgc cattgcaggc aagacacttg ccttcgattc gatcttttca
     4741 aacaagttgt ggttgctcat accggccctg acgctggtga cagcgttcct ggcaggcctg
     4801 tatccggcct tctatctcac ctccttcaat ccggtggaag tattgaaagg tatccggcta
     4861 ttcaaggcga atatcggcaa cctgctgatc agaaatggac tggtgatcat tcaattcacg
     4921 atcgccgtgg tattgatcat ctgcacgctg atagtattcc agcagatgaa attcgagcaa
     4981 aacaaagacc ttggtctgaa taaggaaaat gttttggtcg tcgaaaacac caaacgcctc
     5041 gggaataacg aagaatcctt tcgacaggag cttgcaaaac aacccgggat cctgagtgcc
     5101 tctatctcca gcggtatccc taccaaaggt agttttggcg atggatatgt tcccgaaccc
     5161 gccgggggag acaaaccctt gctcagcgat atcgggcttt cttcgtttat ggtcgatgat
     5221 gcctttgttc ccacataccg cctgcaaatg ctgaaaggaa ggaatttttc gaagacattc
     5281 aatgactcgg catcggtgat catcaatgaa accgccgcgc aaaaaatagg ctggaaagac
     5341 ccggtgggaa aatatcttgc ctatccgggt aacgctcagc ggttcaaagt gatcggcgta
     5401 gtaaaggact tcaatatcga atcccttcac gagatagtcg aaccctttgc cttattccac
     5461 aactcctccc acacatacgg tctgttttcc tcgtatgttt cggtgagatt gcaacccgga
     5521 acgatcagcc ggcagatcgg cagtataaag acggcctggc agaatttcgc cccggccacc
     5581 ccgttcgact tcagcttcct ggacagcgat ttcgacgcat tatatcgctc cgaacaacag
     5641 atgggcgccg tcttccgcat ttttacattt ctttcgatct tcgtggcctg tctcggtctt
     5701 ttcggcttgt ccatctttgc tgcacagcga cggaaaaagg agatcggtgt cagaaaagta
     5761 ttgggcgcat ccgtggggaa tgtggtcagg ctcttgtccg ccgattttat aaaactggta
     5821 gccgtggccg cgctgatcgc tttcccgatt gcctggctgg ctatgaacaa atggctcgcc
     5881 gatttcgcat accgtataaa catcagctgg tggatattcc tgatctccgg cctgattgca
     5941 atagccatcg cattgatgac cgtaagcttc caggcgatca ggtccgcaac gtcaaaccct
     6001 gtgaacagcc tgcgatcgga atagcatgaa accatacact atcaccctcc tgctctgcat
     6061 ggcattcatc cgcgctcatg cccagcaacc ccgcaatctt atcctgtccc acggcgcgat
     6121 cctcagaggc gactctacaa aaaagaccat agccctcgtc ttttccggtg acgaatttgg
     6181 cgacggcctg ccggcgatca ttaaagccct ccacaaacac cacgtaaccg gatcgttctt
     6241 cttcaccggc cgcttctacc gcaatcccgg gtacacatcc tctatccaac agctctaccg
     6301 acaacacaat tatctcggcc cccactccaa cggtcacctg ctgtacgccg actggaccca
     6361 tcgcgacagt acactcgtca ccgaagacag cttccgcacc gatcttcaaa agaatctcgc
     6421 cgccatgctc gcccttggca tcgacacttc cggtccgcat tatttcatac ctccttacga
     6481 atggtggaac gatacgatag ccgcctggtg ccataccatc ggctggcacc tgatcaattt
     6541 cactcccggt attcgcacca acgccgacta tacctatccc gaaatgggcc gcagctacct
     6601 cagcagcgaa gccatccttc gaaccctcaa aaagtaccgc ccctccggcc tcaatggagc
     6661 catcatcctt atccacgccg gcaccgaccc ccgccgcacg gataaactct acaaccgcct
     6721 ggaccaattg ctgacccagc tgcaatccga cggctatacc tgcctcccga tctcaaaatt
     6781 cttctccgat tagcgtacct taggaggcaa gaccctgcat atggataaaa ggacatttat
     6841 gaaaagcatg acgctggcgg gcctcggcct gccattcgcg caaaaaagcg atccccggcc
     6901 ggcaacactc gccgacctca aagacctcat cagccaatac ggcgaaatag cacccgccac
     6961 cctcgcggaa aacgaagagt tctggacggg catccgggcc cagtacaatc tcaagcccga
     7021 ctatgccaat ttagagaacg gctattacaa tatccagccc acacccgtat tggaagcatt
     7081 tttgcgccat gtgaaagacg tcaactatga gggcgccttt tacatgcgga ccgtacagtt
     7141 cgatcgaaaa aaagcgattg ccgccagact cgctgccctc ggcggctgca caaccgaaga
     7201 gcttgtcatt acccgaaata ccaccgaatc cctcgacatg atcatcgccg gctatccctg
     7261 gaagccaggc gacgaagcca tcgtggcgga acaggactat cccgccatgc tggcgatgtt
     7321 cagacaggtc gaaaagcgtc acggtataat agtaaagcgg gtctccgtcc caaaccatcc
     7381 ggcatccgat gcagacgtga taagcgtcta tgagcaggcc attacccccc gcacccggtt
     7441 gatgatggtc tgtcatatga tcaatatcac gggtcaggtc ctgcccgtgc gcaagatctc
     7501 tgacatggcc cattcgcatg gcgtcgaggt catgatcgat gcggcgcata cctatgccca
     7561 tatccgtcac actatacccg aattaggcgg cgactattac gcagcaagcc tgcacaaatg
     7621 gctgagtgcg cccctcggcg ccggtatcct gtatgtgaag aaggaacacc aggcgaagat
     7681 ctggcccctg ttcgcagaac cggatggctc cgctgagggc atcgcccgtc tcaaccatac
     7741 cgggaccctg cccgtacata ccgatcttgc catcggcgat gcgttggatt attatacaac
     7801 tctcggggcg gaacgcaagg aggcgcggct caggtatctt cagcaatact ggacctcgaa
     7861 gatcagggat gttccgggca tcgtcgtcaa cacccctgcc gatccgacgc gaggctgcgc
     7921 gatcgccaat gtcgggataa agaacatagc tcccggcgat atggctgccc gtctccttca
     7981 gcagtatcgg atctatacgg tcgctatcga ctatcccgcc gccaacgtcc gcggttgtcg
     8041 cattacaccg aatgtctaca ccctgacgac cgaactggac agactcgtgg cagctctccg
     8101 cgggatggca tcggcgtccg cataatcccc ccttcgtcgt catcctctca aaatattgaa
     8161 atgaactggc ttaccctgct tctcttcttc caaacactcg gtcatccgac tccgccgccc
     8221 caccgtcacc cgactccgcc gccccacctt cacccctcgc cgacgcccca ccgtcacccg
     8281 actccgccac ccgacagtcc cttcgccatc ctcacccgaa aacaggaggc cttacaccat
     8341 ctgcccgtcg gtgcgatccg tccggaaggc tggctcaaag accagatcct ctccaatctg
     8401 ctcggcttca caggccacct cgacgacctc gcgcccgacc tgctgatccg cgatgatatc
     8461 tttggcgccg acaggctgac caaaaaaata aaaaagaaag acgtcggcgc cctcgccggc
     8521 ggtgacacca gcgcgcagat ccagtacctc tggtggaaca gcgagacaca gggcaactgg
     8581 cgggacggct tcatccgcag cgccatcctc acaggcgacc ccgccgccat aaaacgcgca
     8641 cgtatatacg tccggcgcat ccttgccacg caggacccgg atggctacct cggtatctat
     8701 gacgaggacc tccgttatca cttccataca gaaaatggcg agctatgggc aaaaactacc
     8761 ctgctacgcg ggctgcttgc ctggtatgaa tatagtcgcg accctgcggt gctcaaggcg
     8821 gtggagcggg ccgtagcgga cgtaatatgc aattatccgg cgggtcactc tcatcccttc
     8881 tattcgacaa ccgcgtacgt aggcggcctg tcacatgggc tgatgttcac cgatatcctg
     8941 gaagagctgt tccggatcac cggccgcacg tcctggctcg attatgcggt attcctctac
     9001 aaggacttct caacccaaca cctgaatgaa gacgcgcagt attccaggat catcgatccg
     9061 gcttatcgtc ttcagggcca tggcgtgcac acctatgaac acctgcgtgc cgtggcagcc
     9121 gcagcctggg catcgggcaa cccaagcctg ctgtcggcgt tgaaaattta tctcaccagg
     9181 atatccaatg agctgatgcc ttccggcggt ccggccggcg atgagtggat cggcaataga
     9241 accggtgatg cgacgaccac cggttatgaa tattgttcgc tgcaggagtt gatggacggc
     9301 tatatcgatc ttatgcaaaa gaccggctcc gcatcctatg gcgaccgtat cgagcggctt
     9361 ttcttcaatg ctgcccaggg cgcccgtgat ccgcaggcct cctgtatcgc ctatcttaaa
     9421 accgacaact cctaccagat gaccggcggg ctcaacggcg atacgacagc gcatcagatc
     9481 aggtataaat attcacccgt ccaccaggat gcggcggtgt gttgtgtgcc gaatgccgga
     9541 aggatcgccc cttatttcgt agacgggatg tggatgatcg gcagcaccgg tcccgttgcc
     9601 accttgctgg gtccatgtac ggccagcatc acctggaaag cccagccgct gaagatcagc
     9661 gaaagcacgg gctatcctaa tgactttacc ttcgacttca ggatcaccgc cgcccacccg
     9721 acaaccttta ccctcagcat ccgcaaacct gcctgggcag acaggttcac gctcaacgct
     9781 ccatacgtcg aaaaggacgg gtacatccgc gtgacgaaga cctggaagac cggagaatcc
     9841 atccggcttc gattgctccc tgacgtgaag accgccaacg accgcaaagg ttcaaccttt
     9901 ttcacctatg gccccctcgt cctcgctcat cctatctccg ccgcaggcac gatcaccaaa
     9961 cgctatcccc tgcccggctt ttatgactat cagtatcgcc ccgacagcct tactctatat
    10021 cactatggcg gtgaaaagat ctcccgttca cccgatccct ataatggctt tggcgccata
    10081 cgcttcaaaa cgtcgctgta tacagaccac gcgagccgca aggacagcgt ctcgcttgtc
    10141 cccatgggcg ggaccatcct ccggcaagtg accttctagc cggtgcgttc cgggttcatc
    10201 ctttgcataa tccattgtat gcccgaaggc ccgtcgatcg tcatattgaa agaacagacc
    10261 caaaaattct ccggcaggaa aatattagag gtggacggca atacaaaagt cgatctcacc
    10321 ggcgttgccg gcagcaagat caccgctttc gccagctggg gcaaacactt tctcatcgtc
    10381 ctgcccaaaa ggaccatccg cattcatttc atgctcttcg gttcatacag catagacgaa
    10441 cagaccaaac ctacgccaag acttcacctg aagttcaaaa ccggcgacct gtatttttat
    10501 gcctgttcgg taaaggtcct ccccggagac atcgattcgc aatacgactg gagcgccgat
    10561 gtgatgagcg acacctggga ccctgcgaaa gcccggaaaa agctgaagac ctgcccggag
    10621 gagctggcgg ccgatgccct cctcgatcag gacatctttg ccggagtagg caatattatc
    10681 aagaatgaag tccttttccg gatacgcgtc cacccggaaa gccggatcgg ggccctcccc
    10741 gctcgcaaac ttacccagct gatcagcgaa gcccgaattt atagtttcga tttcctgaaa
    10801 tggaaaaaag ccttccagct cagaaaacac tggctggcac atgcgaagac catatgcccg
    10861 cgcgatcata taccattcgt caaagtaaaa cacctcggaa aaagaaaacg gcgggccttc
    10921 ttctgcacca aatgccagcg gctctacacc tagctggcaa taaatttctt taatatcttc
    10981 atcaacactt tctcttcagc cgtaagcccc gcatggtcct ctgccacctc ttccttaaga
    11041 taattagcca gcttcttttc ctcatacagc ctgatcagcc ccggatgcac ataatatttt
    11101 ttgcagacgg tcctcgtatt tcccaaccgc gagctgacgc tatcgagaac gacatttata
    11161 ttccttttcg tcgccgcgtc cgtccctgcc tcgcccaccc ggatgaattc ctgcaacgca
    11221 tgcagcgaac ctgcccaggt ccgaaaatcc tttgccgaaa aatcttctcc ggtggcctca
    11281 cggatatagg tattcaccat cccggaatcc accgggtgcc gggtgccatc ctctccataa
    11341 tactcgaata attccttgcc ggggatatcg tggcattggg cgacgatccg cgcaagccgc
    11401 cggtcacgca acgtgacttc atggtgtatg cctttcttac ccgtgaacga aaagctgatc
    11461 ttatcccccg aaatagaaac gtgtttattc ttcagggtcg taagaccata tgagccgttc
    11521 gccttttcgt attcattatt acccacccgc ataaaagtcc gatccatgag gctgacaaca
    11581 gtagccaaca ccgtgtcctg ggtaagaccc tttgcccgca ggtcttcctc tacccgacca
    11641 cgcaataaag gcagggcttt cccgaactca tacaggcgat gaaattttgt ctcattgcgc
    11701 aaagaattcc acagcggatg atacctgtac tgtttgcggc gccgaagatc gagccccgta
    11761 gcctggatat gccctttctc atcggcacag atccacacct gcgtccaccc cggcgggatc
    11821 acgagtttcc ggatacgctc cagctgcccc gggtccccga ccaccttatt gttgaaaaga
    11881 tagcggaacc ccttcccttt cttaacgcgc aagatccccg gctgacaatc cctcacatag
    11941 cgcaggtgag ccaccgaggc agccttttca tgatccctgt ccacccgaag gaactcttta
    12001 tgagagaggg ccagtgtagt tcccgattcc atgtgatgcg tacttcataa atgatgccgt
    12061 atcgcaacac ggccatttaa acaaaaattt ggccgcttct gtattatgat tatatttaat
    12121 acctaaactc aaaatcgatg aacctcttca aatctggtag ctcgctcctt ttcacagggg
    12181 ctgtaattct tctgacctca tgcggatccg gtggcaacga caaaacaacc accgctgact
    12241 ccaccgctgc cgccgacagc gccgcgaagg caaaagcggc aagcaccatt gttacgacac
    12301 cgacgaatat cgtcgaagta acacacaaag tcgccaacta tgaaaaatgg ttagccgcct
    12361 atgaggcgca tgactccgcc cgactggctg cgggattgca caattatgtc attggccgcg
    12421 gtctcgaaga ttccaatatg gtcctggtgg cgttgaaatg tgatgacacc gctaaagcca
    12481 aagcatttgc aaaagatccc ggtctgaagg cagcgatgaa aaaaggagga gtcctcggca
    12541 ccccgacaat ctccatctct accgaaacct ggcaggatac cgtaaccctc gctcccaaca
    12601 ccattcgttc agtggccacc ttcaccgtca aagactggga tgccttcgtt aagcaatttg
    12661 agagtgacaa acaggaacgt ctcgataatg gcatcatgga ccgtgtggtc ggccacgatc
    12721 tggacgacaa taaaaaagta tccatagtaa cggcgttatc ggattccgcg aaagccaccg
    12781 cataccataa atcggatgcc ctgaaaaaaa gaatggaggc agccggcgta gtaggggaac
    12841 ccgccaggtt tctcttccgc atcgtaaaaa tgaactagtc cctccccgac ctcgggtgga
    12901 tcaaaatgaa gcaaccgctt catttttttt tgcgctaaat ttcaaagaat aacctccttt
    12961 tccaacacca ccagctccaa cgccctcttc tgcgttccaa atctatcccc cgcatgaaaa
    13021 gcctccacct caccagtcct ctgaaatccc tgctggtcca tcccccttct ccccgataag
    13081 ccctgttcac caggccgacc agttccgtta tatcatcctt cgttgcggcg cgaatttgca
    13141 taaattaatt tttactccac cttatagatc gcgtcgaaat cctgcttccc ctccttcggt
    13201 tccataacgg taaactccaa tttcgtattc cccgtcttat acaaccccca tatcatatcg
    13261 gctcgaacga aataactatt gccaaatttg atctccatcg gcaagtcgcc ctcgaccttc
    13321 gtctcgctaa cgcgtgaccg caacgtaaaa gctccttctt taaaaatagc cacaacagcc
    13381 gccgtcttgt ttttcagcac gaccatgggc aggtcattat aatagatcag cagcggcgag
    13441 aaagacaatt tcaactttcc cggacgatag atgtaaagaa gagccatccg cgaagtatcg
    13501 ctgaaatgta tcttccggat ccgacccgga tcggtaatgc caaacaccat gttttcctcc
    13561 gtatgcgtct gcgggtgcat ccacatcgta tcgttcgcca gtagattttt cggcgaagcc
    13621 cggtcaatat agctactgaa tacaaaataa tccggtccga tgccccctac atgataagcc
    13681 accttgtgct tagccagaaa tttcagatca tcctcgccca gaccgctgcc ctgctggtat
    13741 ttcagcgtat agtcgcaatc tcccttccac tcgatcttgt atacacttga atcataatat
    13801 gaggcgccct tgcgcttatt caggttcacg actttctcca tatcgccgga cactaccaca
    13861 tggtactgct gaggcgtatt ccgcggatag aaataatatt cccccgaatg catatctgca
    13921 cacgaatgct gggccgagac agatagagga ttcacccaca cgcccccgaa tatcaccgct
    13981 ccaaacagaa catacattat tttcatggtc aattttccca aatgtagtac cttctcaccg
    14041 atgaaaaata tcctttccct ctgcctgatg ctcggcatcc tctctacgac gatcgcccag
    14101 cctctccata agaaatatga tctcctcatc acgaacgcca atgtcgtcga tgtccctacc
    14161 ggtaagctcc tcaaaaaccg gctgctcgcc atatccggag atacgatcag ggccgtggat
    14221 gacacccgaa aggccgcgac ctacaaggcc gacaaagtca tcgacgccaa tggaaaatat
    14281 attatacccg gcttatggga catgcacatc catctgcgcg gcggcgattc caccatcgag
    14341 gcgaataaga agctattgcc cttgttcctc gcatatggcg tcacgacagt gcgggaatgt
    14401 ggcggagata tcacgccttc cgtcatgcgc tggcgcgctg agacggccaa tggagagctc
    14461 gcgggtccac ggatctttac ctccggcccg aagctcgacg gaccgcacgc agtatgggcg
    14521 ggttccatcc ccgtcgtaac gccacaggat gttacacatg cgctggactc actgcaggag
    14581 atccattccg acttcgtcaa aatatacgat agcaagatct ccggcgatgc ctatctggag
    14641 atcatccggc aggcaaaaaa gagaggcatg aaggttacgg gtcatatgcc ttacaccgtc
    14701 gagctaaagg aagcagccgc actcggcatg gacggctcgg agcatatgta ttatgtcttc
    14761 aaagcctgtt cgtccaaaga ggacagcatc accaatctgg tccgtgagcg ggaacacacc
    14821 gcccgcccga tgggcctctt cggtgccctg cccgccttat atgctacatt cgatagcgtc
    14881 aaagccgcac aattgttccg ttatctggcg gcgaaacatt ttacgataac tcccaccctg
    14941 ttcatctcga agacgctcgc cggcatcaaa gacaccgacc acagcaagga tagcctgctc
    15001 aactatatcg atcctaagat acaggccacc tatgcagggc ggatcagagg agccaacaga
    15061 caatccgatc aggcaagaca gttcacagaa aaa
//
LOCUS       DASYSV010000096        17623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2068855, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000096 DASYSV010000000
VERSION     DASYSV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 17623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17623
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068855"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..950
                     /gene="sufC"
                     /locus_tag="VGQ51_02910"
     CDS             177..950
                     /gene="sufC"
                     /locus_tag="VGQ51_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV9035538.1"
                     /translation="MLIIKNLHAEVEEKQILKGIDLTVRPGEVHAIMGPNGSGKSSLA
                     SVLAGKEGYHVTEGDVTFRGKDLLDMDPEIRAREGLFLAFQYPIEIPGVSNVNFLKTA
                     LAEIRAYRNQPALDAKEFLRMSREKQKLVEFDAQLVNRSLNEGFSGGEKKRNEIFQLA
                     MLEPSLAILDETDSGLDIDALRIVAGGVNKLRSKDNAFIIITHYQRLLEYIIPDFVHV
                     LYNGRIVKSGTKELALELEEKGYDWLREESREQEATITQ"
     gene            947..2260
                     /gene="sufD"
                     /locus_tag="VGQ51_02915"
     CDS             947..2260
                     /gene="sufD"
                     /locus_tag="VGQ51_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV9035539.1"
                     /translation="MSTQHETAYPLYTEVVNEFDKLTAAGRADKARQTAFERFRLLGF
                     PSIKHEDWKYTNITRFLKDEYALVPTVREIPAGLVEKATIPNLDCYQLVLVNGIWDGQ
                     VRGGNLPGGISIRRVNEALRDPGTAALFENGKWGNQHFANLNAALFTDGLFITVDKGA
                     DAAKPLHILHLYTTDHNLLVQPRHLWVVGANAGLSVIESTVCTTTGEAKVWINGLTEI
                     VAASDARIDHVAIQTATAGTRLVQHTTVRQKKQSLYNNYTFTLPGADLVRNDLQLRLE
                     EDHTETHLYGLYLTAGNQLVDNHTLVDHQKPFCFSNELYKGVLLEQSTGVFNGKVYVH
                     KDAQKTNAFQQNNNLVIGKRATIDSKPELEIYADDVKCSHGSTVGQFSEEALFYLRSR
                     GIGEATAKALLINAFAFDVTEKLKIGAVETYINQLIRRHIPAEHA"
     gene            2253..3533
                     /locus_tag="VGQ51_02920"
     CDS             2253..3533
                     /locus_tag="VGQ51_02920"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEV9035540.1"
                     /translation="MPDPATIMSTGGEARKPESPALDINVIRKDFPILFRTVYGHPLV
                     YLDNAATTQKPEQVLRAMDDYYHTINSNVHRGVHYLSQQATEAYEGARQKISDFINAR
                     HNHEIIFTRGTSEGINLVASGFAKKILQAGDNVLVSAVEHHSNIVPWQMACEEKDAHL
                     RVIPVDENGVLVIDGLDRLLEGVKIVAFNYVSNSLGGINPVREIIEAAHRRGIPVLLD
                     AAQAVQHLEVDVQSLDVEFLAFSSHKLYGPTGIGILYGKEEWLHRLPPYQGGGEMIKT
                     VTFEKTTYNDLPYKFEAGTPDMAGAIGLAAAIDYVQNLGLRNIHAAEVSLMSYALDTL
                     HEIEGLRLIGNAAADGRAGVISFLVGDIHPFDMGEVLDKQGIAVRTGHHCAQPVMDRY
                     CIPGTVRASLAIYNNREDIDRLAAGIRKAAKLLG"
     gene            3566..3994
                     /locus_tag="VGQ51_02925"
     CDS             3566..3994
                     /locus_tag="VGQ51_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEV9035541.1"
                     /translation="MTIKEQQDEIIADFELFGDWMDKYEYIIQLGKELPMIDDKYKVD
                     ENLIRGCQSRVWLHADYSEGKLLFTADSDAVITKGLVSLVIKVLSGHTPKEIADADVY
                     FVDEIGLSSHLSPTRSNGLLSMLKQIKMYALAFQSKNLQS"
     gene            3991..4317
                     /locus_tag="VGQ51_02930"
     CDS             3991..4317
                     /locus_tag="VGQ51_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEV9035542.1"
                     /translation="MSETTSPAPTLRDEIEVQLRTVFDPEIPVNIFDLGLVYEIKVGD
                     NGAVKITMTLTAPACPVAGEIVREVQEKIEALPEVLDCHVQLTFDPPWTKDMMTEEAK
                     LELGFL"
     gene            4600..7845
                     /locus_tag="VGQ51_02935"
     CDS             4600..7845
                     /locus_tag="VGQ51_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9035543.1"
                     /translation="MKFFSPKEWFFRPLSWLLAVLLLAGPVLAQGQGAPKITVTGKVL
                     DDASQPVAGASVMIKGTRNAVTAGADGSFSIVTVRGATLVISYVGFQDKEVKVTGAAV
                     GPISMAKGDKSMEDVVVIGYGTQKRQNVTAAVATMDTKSIQERPVTRIDQAMIGQMPG
                     VQVRQQTGMPGSGFTILVRGTGSISAGTEPLYVIDGFPLDVNSVNTAGGFSNGNPLNN
                     LNPDDIESIQVLKDAAAGAIYGSRAANGVVLITTKHGQIGKPRLVVNANAGVSNVARK
                     MDLLDAKDWIALMTDLTNYKWVNSGTGRTADQSIAQRQAILNLGPNVYNTTYMADPRW
                     FQPGHPGLEYVNWQDSAFRAAPFQNYQVSVSGGSENIRYFVSGNYLNQEGTLLNTGYT
                     NYSVRANVEATVAKRLKLGFNIAPTYSITQAPSAEGKDNQLAHLYNMIPVVEDSAGMN
                     SGAGKNNVYGWAGSSVSPVAYLNNVIATNKTTRNLYTVYGEFTILDGLTARSTFNYDQ
                     MNQSTKSYISDFVAGNITDRLNSPGKDASGSYAGYTKQNFVNENTLNFNRTFHEDHSI
                     SAVAGVSYSYVHLENFSIKTAGGFANDLLNTLNNAIPSTAGVTVTGNTTESNNALFSL
                     YSRVQYSYQGKYLVSASIRRDGSSRFGKDNEYGTFPSVSVGWRISQENFMRNVNFIND
                     LKLRASWGKSGNNNIGDYSAIPTLTGANYSFGGNTAAAVTGQRPNGLPNPFLQWETSN
                     TYDVGFDATVWHSRINIIVDAYRKKNTNLLLTVPVPAASGTTSNLENVGSVQNQGLEF
                     GLGATIIRGRDLTWTLNGNIAFNQNKVLSLGGLGDVINIPNAFGTGYPPFLLQSGKPM
                     YSYYVIKNIGILSQDDINNPKVTKMPKQAVGDEKYLDVNGDGIIDASDRVIDGQPTPK
                     YTWGLSNTFRYKSLDLNVQLYGQHGGAILSYMARAIDNPGNGKATNLGVWRNRYSYYH
                     QSLHSPRGNIGYAYSIPYTTTDWLYSTDFWRIQNITLGYNFRNIAKTHVISTMRVYAT
                     ALNWFGWDKYKGGVNPESQNTNLTNGTYPLPGDYGAMPLSKSIILGVNVGF"
     gene            7902..9440
                     /locus_tag="VGQ51_02940"
     CDS             7902..9440
                     /locus_tag="VGQ51_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9035544.1"
                     /translation="MNTVKYSIYSILIMGLAMAGCSKQLNQQPPSSIVTSLFYSNTND
                     FTQAVNGVYNQLRNYPDQELWMGEMRSDNIVATSDGNRDWQAINDFLPNLTTVSFITS
                     GWDNNFNGIYNANSVLDNLVTKGSNVTDPNLRARFGAECRFLRAFYYFQLVRLYGAVP
                     IVTHVMSATDVVKVKRSPVADVYKQIIGDLDTAITGLPNSYTGVDVGRATKWAAEGLL
                     GLVYLTRSGPVYQGVDGPTMGTGEWDQALTHFNNVINSGQFKFNASYPGIFSYTGENN
                     PEVLFDVQYMSTNNGADFPSMLVNSGYWGGLGISGSYGNGYGSSTYDVSKNLRASYAA
                     SMGSATDVRDTFNIQWKYNTNTTNPIVTDTSDPFEKKYINISKRGTGYNDWPINFIVM
                     RYTDVLMMKAEAILNKPSLGSQATVDSIVNLVRTRAGVAPMTNVTLSQLMEERRREFL
                     GEGLRWNDLVREGMAVTTMNAWIASDGITKDQQVIPAFVIYPVPEAEILAVNGLYTQN
                     PGYQ"
     gene            9437..10624
                     /locus_tag="VGQ51_02945"
     CDS             9437..10624
                     /locus_tag="VGQ51_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HEV9035545.1"
                     /translation="MRTLIIFAAVFFCRGVAAVAQPLEETGPKKAMPDEWIDQDTHHK
                     VVRLSRKDGNNLSFYFHNNPFLDGKMIFYSTDESGKWLYTVDLKTLALTRVSKTTGNS
                     EIVGHHSGSVYYQSHDSVFNTSIGSGKTKLVFVFPADFKGNITTLNADETLLAGGWAS
                     EEQREISRKYPEKRDFFDRIFDAHLPNKLFTIDLRTGQLRVIHTENTWLGHIQFSPTD
                     PDLLMFCHEGPWHKVDRIWTIRMKSGDVRLMHKRTVDMEIAGHEFFSPDGKTIYFDWQ
                     IPKGQTFYLGAVDVATGAEKKYAITRDEWSIHFNVAPNEKLYCGDGGDAGQVAHAKDG
                     RWIYLFRPEGDSLAAERLVNMKDQYYKLEPNVHFSPDGKWVIFRANFEGKEEVYAVEC
                     KKS"
     gene            10631..13399
                     /locus_tag="VGQ51_02950"
     CDS             10631..13399
                     /locus_tag="VGQ51_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEV9035546.1"
                     /translation="MRSLLLFVLLTVQLSVSAQLQWPAITAQTKPWTRWWWMGSAVDK
                     ADLTANMEKYAAAGLGGLELTPIYGVHGYENQFVDYLSPKWMDLFGYTLGEARRLGLG
                     LDMTTGTGWPFGGGPLIDSTYACREMFHKLWAVKGGEGLPDSIRYQQEGYVHTDGRPT
                     TIAQLVEPVFANKDRQALALFQVRFPHSLPPYSLMAYSGDGRVLDLTNRVDAKGHLDW
                     TAPAGSDWTLYGLFLGWHGKMVERAAPGAEGNVIDHFSSVALRKYLSRWDTAFGHLDL
                     SPLRCFFNDSYEVDDSRGQSNWTPGFFDDFRHLRGYDLREHLPALFGKGNSEDIARVI
                     CDYRETISDLLLQQFTQPWHDWAHAKGARIRDQAHGSPANILDLYAAADIPETEGSEI
                     LRYKFATSAAHVTGKPLASAETVTWLNEHFLSSYGDVKTALDNYFLGGVNHVFYHGTA
                     YTPMADPWPGWLFYASVHFTPNDPAWPDVSTLNRYVARCQSFLQKGAPDNDILLYYPL
                     YDSWSEPGNVLLKHYDRMDPEFTGTGFMTCADFLQHHGYTFDFISDRQLQGVTAGAGG
                     GIVTGGVTYKTILLPDCRYLSVDALKKLVGLARHGVKVLVYKRLPYDVPGWGHWQERE
                     TQFKELLSGNNFAVGEDLDSLLTTANVHREKLIDDSIGFFRRRLGSGEVYFIVNKGQT
                     AFAGYLPLDRVFRSAAIFDPMTGGKGMARRRGDSVYVQLRRGETCIIQTNPAGGASLF
                     PYYLPMGGSMRLTGRWHLEFLSGGPVIPHDTTVGMLGSWTDLGDEAMQNFSGVGKYSI
                     DFDRPATGVQAWLLQLGKVYRTVEVFLNGHKLTTLLGPDYEVVIPSSAMHQRNTLEIK
                     VANTMVNRIEYMDRRHILWKKFYNYNFPAHERANRGADGLFDTSKWAPMDSGLSGPVT
                     LLPLRLLP"
     gene            13417..16557
                     /locus_tag="VGQ51_02955"
     CDS             13417..16557
                     /locus_tag="VGQ51_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HEV9035547.1"
                     /translation="MVYALILAPGFVPAQVRLAAAKGGPGKGHSVSAEGGPADHMVRY
                     HPEGGDFVIVNGTRRFNRALYGTHTAFRVEAGDLPEFAFYMPGMGGNLHFELEGRPLI
                     RSQYIKAIYRPGSMLYEIRDPALGAKGVLSMTVLALADAEGMIIRISGRNAGRHLGVV
                     FGGASGKKFSRDGDIGADPESSFYLDSAGCRGDHYVVRGDGWSLEYGAGKKLEGIFPP
                     GSGLRVDAVPALHGQLSLGDTCYLLIRNPASASASLPSYSELPAFFDSAETVRERLAG
                     RVVLHTPDPWINTLGGALAVAADAIWEDPSYLHGAVAWRQRLPAWRGPYVADPLGWHD
                     RARRHFSSYALSQLTEPATAPVVADTALHLARQLERLGTSLFSSGYICRNPGGDLRPH
                     HYDMNLVFIDQLLTHFYWTGDTAYVRQMWPVLTRHLAWEKRNFDSDGDGLYDAYACIW
                     ASDALEYGGGGVTHSSAYNYRANKLAADLARVIGEDPAPYAAEAAHILNAINSRLWMT
                     EKGWYAEYIDWAGGHGTPVLHPSAGLWTIYHAIDEGVADPVQAWESLKYIDRYIPHIP
                     IRARGLGDTTLYLLSTTNWQPYTWSLNNVALAESLHTSLAYWQGGRPDEAFRIWKSAL
                     VESMYMGSSPGNFEQLSSYDVARGELYRDFGDGIGMAARSLVEGLFGISPDAIHDTLT
                     IAPGFPGEWTKASLHIPDISVEFRRQGRTDEYHIFPNFPRHMNLRLRLHAQAETIRRV
                     VVNGKPASWKKIVEEVGVPVMEVDVPPATEYHVSVSWGGGPAFGIGIALGGPRLRTYT
                     GGSDLPAKGEHADTVSLTGYFNDDVTHIFQQQYLSPRPKSPTLQLPTQGIGNWCYPLV
                     NADIDDAGLRASGGEVRLPGGIPFATPASGHNIVFTSLWDNYPDSVSVRLGGQAKHAW
                     FLMAGSTNPMQSQITNGVIDVYYTDGTSDRLELVNPSNWWPIEEDYLEDGPAFTTGAP
                     HPWRVYLKTGVVSNQPGKWTTIKGLTNTAIEGGAATVADMTLQPDKTLDHLVLRTTAH
                     DVVVGLMSITLIKKNE"
     gene            16554..>17623
                     /locus_tag="VGQ51_02960"
     CDS             16554..>17623
                     /locus_tag="VGQ51_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEV9035548.1"
                     /translation="MMKYLLILLLMPWSGPERGGKAGADPDWTTKVGAHEFPHGKAMV
                     TVTGAAKDGSKLATHAIQSAIDDCAAKGGGVVVFAPGIYQTGSIFLKANVELRIDKGV
                     ELIGSQHFEDYPEIDTRIAGIEMRWPAALLNIIGVKNAAVTGEGKVDARGKFCWDKYW
                     AMRKEYDAKGLRWIVDYDAQRVRTLLIQRCGDVTIKGVTFVDAGFWTVQVLYSEHVTV
                     DGIVVRNNEGGHGPSTDGVDIDSSSWVLVQNCDIDCNDDDFCLKAGRDWDGLRVNKPT
                     EYVVIRHCVARKGGGLLTIGSETSGGIRHVLATDLTARGTGNGFHIKSATTRGGTVED
                     IHVKNITMDSVGNAILFTMNWNP"
BASE COUNT         4102 a         4519 c         5136 g         3866 t
ORIGIN      
        1 aacggatatg cgcgtgaggt gctgaaccag ctgccgatgg aatttgccgt cgaggctcaa
       61 aagctattat cgattacact ggaagggagc gtaggatagg ggagggagct ctcaaaggaa
      121 gcgtaggtta aacaataatg acggacttcg ggtccgtcgg cccaaaacaa aaataaatgc
      181 tgatcattaa aaatttacac gccgaagtcg aggaaaagca gatactcaaa ggcatcgatc
      241 tgacggtaag accgggtgaa gtgcatgcga tcatgggtcc caacggctcc ggtaagagtt
      301 cactggcgtc cgtacttgcg ggaaaagaag gttatcatgt caccgaaggc gacgtcacct
      361 tccggggtaa ggacctcctg gatatggatc ccgagataag ggcccgggaa ggattgtttt
      421 tggctttcca gtatccgatc gagattccgg gtgtgtccaa cgtcaacttt ttgaagacgg
      481 cactcgccga aataagggca tatcgcaacc agcccgcgct ggatgcaaaa gaatttctgc
      541 ggatgtcacg cgaaaagcaa aaactggtgg aattcgatgc tcagctggtc aaccggagtc
      601 tgaatgaagg gttttccggc ggcgagaaaa aaaggaacga gatctttcag ctggcgatgc
      661 tggagccttc gctggccatt ttagacgaga cggattccgg tttggacatc gatgcgctgc
      721 gtatcgtggc gggaggcgtc aataagcttc ggtccaaaga caatgccttc atcatcatca
      781 cgcactatca acggttgctc gaatacatca tccccgattt cgtccatgtc ctttataatg
      841 ggcgtatcgt caaatcggga acaaaggaac tggccctcga gctcgaggaa aagggctatg
      901 actggctgag ggaagaatcc cgtgaacaag aagcaactat tacccaatga gcactcaaca
      961 cgaaacagca tatcctcttt atacggaggt cgtcaatgaa tttgataaat taaccgcggc
     1021 aggcagggcg gacaaggccc gtcagacggc tttcgagcgg tttcgtctcc tgggttttcc
     1081 ctctatcaaa catgaggatt ggaagtatac caatatcacg cgattcctaa aggatgaata
     1141 cgcgctggtg ccgaccgttc gcgaaatacc tgccgggctg gtagagaagg ctacgattcc
     1201 taatctcgac tgttaccaac tggtgctggt caacggcatt tgggacggcc aggtgagagg
     1261 aggcaatctg ccgggcggga tcagcatccg gcgggtgaat gaagccctca gggacccggg
     1321 caccgccgcg cttttcgaaa acggcaaatg gggcaatcag cattttgcca atctgaacgc
     1381 agcacttttc accgatggtc tcttcatcac cgtcgataaa ggcgccgatg ccgcaaagcc
     1441 actgcatatc ctccatctgt atactacgga tcacaacctg ctggtacaac cgcgccatct
     1501 atgggtagta ggcgccaatg ccggcttatc cgtcattgaa tccaccgtct gcaccacgac
     1561 gggggaggcg aaggtctgga tcaacggtct tacggaaata gtcgcggcta gcgacgcaag
     1621 gatcgatcat gtcgccattc agaccgcgac agccgggacc aggctcgttc agcataccac
     1681 cgtccgtcag aaaaagcaaa gtctttataa caactatacc tttacgctgc ccggtgccga
     1741 tctcgtgcga aacgaccttc aactacggct ggaggaagat cacaccgaga cccatctgta
     1801 cggcctttat ctcaccgccg ggaaccagct ggtggacaat catacgctgg tcgatcacca
     1861 gaagcccttt tgtttcagca atgagctcta caagggcgtg ttgctcgagc agtccacggg
     1921 cgtcttcaac ggtaaggtct acgtgcacaa ggatgcgcag aagaccaacg ccttccagca
     1981 gaacaacaat ctcgtcatcg gcaaacgggc gaccatcgac agcaaacccg agctcgagat
     2041 ctacgcggac gacgtaaaat gcagtcatgg tagtaccgtg ggtcaattca gcgaagaagc
     2101 gcttttttat ctccgctcgc gcgggatcgg cgaagcgacg gccaaggccc ttctgatcaa
     2161 cgcctttgct ttcgacgtca ccgagaagtt gaagatcggc gcggtggaaa cttatatcaa
     2221 ccagttgatc agacgtcata taccggccga acatgcctga tcctgcaacc ataatgtcga
     2281 cggggggaga ggcccggaag ccggagagcc ctgcactgga cataaacgtg atccgcaagg
     2341 actttcccat tttgttcagg accgtttatg gacatccgct ggtctacctc gacaatgccg
     2401 ccaccaccca aaaacccgag caggtgctcc gggccatgga cgactattat catacgataa
     2461 acagcaacgt acatcgcggc gtacactatc tcagccagca agccactgaa gcgtatgagg
     2521 gggcacgaca gaagatatcg gattttatca atgcgcgtca taaccacgag atcattttta
     2581 cgcgcggtac ctccgaaggt atcaatctcg tggcgtccgg tttcgcgaaa aagatactgc
     2641 aggccggcga caatgttttg gtgtcggccg tggagcatca ttccaatatc gtcccctggc
     2701 agatggcctg tgaggagaag gatgcccacc tgcgcgtgat cccggtagac gagaacgggg
     2761 tattggtcat cgatgggctc gaccggttgc tggaaggtgt aaagatcgtc gccttcaatt
     2821 atgtttccaa ctcgctgggc gggatcaatc ccgtccggga gatcatagag gcggctcatc
     2881 gccggggcat ccccgtcctg ctggacgccg ctcaggccgt acagcatctc gaagtcgacg
     2941 tgcagtcgct ggatgtagag tttctggcgt tttcgagcca caagctctac ggaccgaccg
     3001 gtatcggcat cctctatgga aaggaagaat ggttgcatcg tcttccgccc tatcaggggg
     3061 gcggtgagat gatcaagacc gtcactttcg aaaaaacgac ctataacgat cttccctata
     3121 aattcgaggc gggtactccc gacatggcag gggccatcgg tctggcggcc gccatcgatt
     3181 atgtgcagaa ccttggattg cgcaatatcc atgctgccga ggtaagcctg atgagctatg
     3241 cgctggacac gttgcacgag atagaaggac tgcgactgat cggaaatgct gccgcggatg
     3301 gccgcgcagg ggtcatctct ttcctggtgg gggatattca tccgttcgac atgggtgagg
     3361 tgctggacaa gcagggaatt gcggtccgga ccggtcatca ctgcgcgcaa cccgtgatgg
     3421 ataggtactg cattcccggg acggtgcggg cttcactggc catttataat aaccgggaag
     3481 atatcgacag gctggcggcc gggattcgga aggccgcgaa actgcttgga tgaacggcgg
     3541 cggcaagcgc tgcaaagttg ttatcatgac tatcaaagaa caacaggacg agatcatcgc
     3601 cgattttgaa ctattcggcg actggatgga caaatacgaa tacatcatcc agctgggtaa
     3661 ggaactgccg atgatcgatg ataaatacaa agtggacgag aacctgatcc ggggctgtca
     3721 gtctcgggtt tggctgcatg ccgactacag cgaaggcaag ctgctattta ccgccgacag
     3781 cgatgcagtc attacgaaag gactggtgag cctcgtcatt aaagtccttt cgggtcatac
     3841 tccaaaggaa atcgccgatg ccgacgtcta ttttgtggat gagatcgggt tgagtagcca
     3901 tctttctccg acgcgctcca acggcctgct ctcgatgttg aaacaaatta aaatgtacgc
     3961 cctggctttc cagagcaaaa atctgcagtc atgagcgaaa ccacctctcc ggcaccgacg
     4021 cttcgggatg aaatagaagt gcagcttcgc acggtattcg atccggagat ccccgtcaat
     4081 attttcgatc tcggtcttgt ttacgagata aaagtcggcg ataatggcgc cgtcaagatc
     4141 actatgacgc tgactgcacc cgcctgtccc gtggctggtg agatcgtcag ggaagtgcag
     4201 gaaaagatcg aggctctccc cgaagtactc gattgtcatg ttcagctgac cttcgatcca
     4261 ccctggacca aggacatgat gaccgaggaa gccaaattgg agcttgggtt tttgtaagaa
     4321 ccgcgtcgag cgcggagggc ggtacgagat gagccgcgtc gagaataact gtcaatcata
     4381 cacttccgac aacgattgcg tagatttcgg ctgaaaagca ggatgcagca ggatagtttc
     4441 cgatctcagt ttttctactt ttatatcgct ggatcaaacg attacttgct atgagcgact
     4501 gcttttcggg gtcattccta actcctattt atccatctct ttgcaatcgg actggcaaga
     4561 cgttccagct ttatttcacc atcaaaacaa cttgccgtca tgaaattttt ctctccaaaa
     4621 gaatggttct ttcgtccgct gtcatggttg ctggccgtgc tgcttttagc aggcccggtt
     4681 ttggcacagg ggcagggcgc gcctaagatc actgttacgg gtaaagtgct ggatgatgcc
     4741 agtcagccgg tagccggtgc gagcgtcatg atcaaaggca cgaggaatgc cgtcaccgcg
     4801 ggtgcagatg gcagtttctc gatcgttact gtcagggggg ctacgctggt catttcttat
     4861 gtcggcttcc aggataagga agtgaaagtg actggcgccg ccgtcggccc catatccatg
     4921 gctaaaggcg acaaatcgat ggaagacgtc gtcgtcatcg gctacggtac gcagaaacgt
     4981 caaaatgtaa ccgctgcggt cgctacgatg gacaccaaga gcattcaaga gcggcccgtt
     5041 acgcgtatcg atcaggccat gatcggccag atgcccggtg tacaggtaag acagcagacg
     5101 ggcatgccgg ggtcgggatt caccatattg gtgagaggga cgggttccat cagcgcgggt
     5161 acggagccgt tatatgtgat cgacggattt ccgctcgatg tcaacagtgt caataccgcg
     5221 ggtggttttt cgaacggcaa cccgctcaac aatctcaacc ccgacgatat cgaaagcatc
     5281 caggtgttga aggatgcggc ggcaggcgcc atctacggat cgagagccgc taatggggta
     5341 gtgttgatca cgacaaaaca cggccagatc ggtaagcctc ggcttgtggt gaatgcgaac
     5401 gcgggggtga gcaatgtagc tcgtaagatg gatctgctgg atgcaaaaga ctggatagct
     5461 ctgatgaccg atctgaccaa ttacaaatgg gtgaactcgg gtacgggccg tacggcggat
     5521 cagagcattg cccagcgtca ggcgatactg aatttggggc cgaatgttta taataccact
     5581 tatatggcgg acccgcgttg gtttcagccg ggtcatccgg ggctggaata tgtgaactgg
     5641 caggactcgg ctttccgggc agcgccattc cagaactatc aggtatcggt gagcggcgga
     5701 tccgagaata tccgatattt tgtgagcggc aactatctga accaggaagg cacgctgttg
     5761 aacacgggat atactaacta cagcgtgcgg gccaatgtag aagcgacggt cgcaaagcgg
     5821 ttaaagctcg gtttcaatat cgcacccacc tattccatta ctcaggctcc cagcgcggaa
     5881 ggcaaggaca atcaactggc gcatctctac aacatgatcc cggtggtgga ggacagcgcg
     5941 ggcatgaaca gcggcgcggg taagaataat gtctatggct gggcagggtc gagcgtcagc
     6001 ccggttgcct acctgaacaa tgtgatcgcc acaaacaaga ccacccgtaa cctttatact
     6061 gtgtatggcg aattcacgat actcgatggt ctgacggctc gcagcacgtt caactacgac
     6121 cagatgaatc aatccaccaa gtcatatatt tccgacttcg tggccgggaa catcaccgac
     6181 cggctgaata gtcctggcaa ggatgcttcg ggttcctacg ccgggtacac gaaacagaat
     6241 tttgtgaacg agaatacgct gaatttcaac cggaccttcc atgaggacca ctcgatctcc
     6301 gcggtcgcgg gtgtcagcta cagctatgtc catctggaga atttttctat aaagacagcg
     6361 ggcgggtttg ccaatgacct gctgaatacg ttgaacaatg ccattcccag cacggcgggt
     6421 gtcacggtga cggggaatac gacggaatcc aacaatgctc ttttctcgct gtattcacgg
     6481 gttcaataca gctatcaggg taaatacctg gtaagcgcct cgatccgccg ggacggttcc
     6541 tcacggtttg gtaaagacaa tgaatacggt actttccctt cagtgtcggt gggttggcgt
     6601 atctcacagg aaaacttcat gcggaatgtg aattttatca acgatctgaa gctacgtgca
     6661 agttggggga aatcgggaaa caacaatatc ggggactact cggcgatccc gacgttgacg
     6721 ggcgccaact atagttttgg cggaaatacg gcagcggccg ttaccggtca gcgcccgaac
     6781 ggtctgccca atccctttct gcagtgggag acttccaaca cttatgacgt tggtttcgat
     6841 gctaccgtct ggcacagccg gatcaatatc atcgtggatg catatcgtaa gaagaatacc
     6901 aatctgctgt tgactgtgcc ggtccccgcg gccagcggca ccacctccaa tctggagaat
     6961 gtggggtccg tacagaacca gggcctggag ttcgggttag gagctacgat catccgcgga
     7021 cgggatctca cctggacgct gaacggaaac attgccttca atcagaacaa ggtgttgagt
     7081 cttggcgggc tgggcgacgt gatcaatatc ccgaatgctt tcggcaccgg ttatccgcca
     7141 tttctgcttc agtcaggcaa gccgatgtac agctattacg taatcaaaaa tataggaatc
     7201 ctgagccagg acgatatcaa caaccctaag gtgaccaaga tgccaaaaca ggcggttggc
     7261 gatgagaaat acctggatgt gaacggtgac gggatcatcg atgccagcga ccgtgtcatc
     7321 gacggacagc cgacgccgaa atatacctgg ggactcagca ataccttccg gtacaagagc
     7381 ctggacctga acgtccagct atacggccag catggcgggg cgatcctgag ctatatggcc
     7441 agggcgatcg acaaccccgg taacggcaag gctaccaatc tcggtgtatg gcgtaaccgt
     7501 tattcctatt atcaccagag cctgcattcg cctcgcggga acatagggta cgcctattct
     7561 attccctaca cgacgacgga ctggctgtac tccaccgatt tctggcggat acagaatatc
     7621 acgctgggct ataatttcag gaatatcgca aagacgcacg tgatcagcac catgcgggtg
     7681 tacgccactg cgctgaactg gttcggctgg gataaataca agggcggcgt caatccggag
     7741 tcgcagaata ccaatcttac gaatggtacc tatccgctgc ctggggacta tggcgctatg
     7801 ccgctgagca aatccatcat cctcggcgta aacgtcggct tctagacgga tcacgggtcc
     7861 gccggccgaa ggcttcgatt caatattaaa agtttaatca aatgaacaca gtgaaatata
     7921 gcatatacag tattctgatc atgggtctgg ccatggccgg gtgcagcaaa cagctgaacc
     7981 agcagccgcc ttccagcatc gtcacctcgc tgttctacag caacaccaac gattttacac
     8041 aggcggtcaa tggggtttac aatcaactcc gtaattatcc ggaccaggag ttgtggatgg
     8101 gggaaatgcg gagcgataat atcgtagcga catccgacgg caaccgtgac tggcaggcga
     8161 tcaatgattt tttgcctaac ctgacgacgg tcagctttat caccagcggc tgggataata
     8221 acttcaacgg catatacaat gccaacagtg ttctggacaa tctggtcacc aagggtagta
     8281 atgtcaccga cccgaatcta cgggcccggt tcggggcgga gtgccgtttt cttcgggcat
     8341 tctattactt tcagctggtg aggctttacg gagcggtgcc catcgtaaca catgttatga
     8401 gcgctaccga cgtggtgaag gtcaagcgga gcccggttgc ggatgtatac aagcagatca
     8461 tcggggacct cgatacggcc attaccggat tgccgaacag ctatacggga gtggatgtcg
     8521 gccgggcgac gaaatgggcc gccgagggat tgctgggcct tgtctatctg acgcgttcgg
     8581 ggccggtcta ccagggtgtc gacggaccga cgatgggcac gggtgaatgg gatcaggcgc
     8641 tgacgcattt caataacgta atcaacagcg gacaattcaa gttcaacgcc agctatcccg
     8701 gcattttctc ctatacgggt gaaaataacc cggaggtgct tttcgacgta cagtacatga
     8761 gcacgaataa cggcgccgat ttccccagca tgctggtgaa cagcggttac tggggcggtt
     8821 tgggcatctc cggcagctat ggcaacgggt atgggtcatc gacatacgat gtgagcaaga
     8881 acctgcgtgc ttcctacgcc gcgagtatgg gcagcgccac ggatgtgcgc gacaccttca
     8941 acatccaatg gaaatataac accaatacta ccaacccgat cgtgacggat acgagcgatc
     9001 cgttcgaaaa gaaatacatc aatatctcca agcgcggtac aggttataat gactggccca
     9061 tcaattttat cgtaatgcgg tataccgatg tgttgatgat gaaggcggag gcgatattga
     9121 acaaacccag cctcgggtca caggcaacgg tggacagcat cgtcaattta gtgaggacgc
     9181 gcgccggtgt cgcaccaatg accaatgtta cgctctcgca gctgatggag gagaggaggc
     9241 gcgagttcct gggtgaaggg ctccgctgga acgatctggt ccgtgaggga atggccgtga
     9301 cgacgatgaa tgcgtggatc gcttcggacg ggataacgaa ggaccagcag gtaatacctg
     9361 cctttgtgat ttatccggtg cctgaagcgg agatcctggc ggtgaatgga ctgtacactc
     9421 aaaatcccgg atatcaatga ggactttaat tattttcgcc gcggtctttt tttgccgcgg
     9481 tgtggcggct gtagcgcagc cgctggagga aaccggcccg aaaaaagcta tgcccgatga
     9541 atggatcgac caggacactc atcacaaagt ggtgcgtttg tcccggaagg atggcaataa
     9601 tctcagtttt tatttccata acaacccttt cctcgacggg aagatgatct tttacagtac
     9661 cgatgagagc ggcaagtggc tctataccgt cgatctgaag acgctggcgc tgacgagggt
     9721 gagcaaaacg accgggaatt ccgagatcgt cggtcatcat tcgggcagcg tttactacca
     9781 atcgcatgac agtgttttca ataccagcat cgggtcgggt aagacgaagc tcgtgttcgt
     9841 tttccctgcc gacttcaagg gtaatatcac cacgctcaat gccgacgaga ccctgcttgc
     9901 ggggggatgg gccagtgaag agcagcgcga gatttccagg aaatatcccg agaaacgcga
     9961 ttttttcgat cgtatcttcg atgcacacct gcccaacaaa ctgttcacca tcgatcttcg
    10021 gacgggtcag ctgagggtga tccataccga gaatacctgg ctgggtcata tccagttttc
    10081 cccgaccgat cccgacctgc tgatgttctg tcacgagggc ccctggcata aggtggaccg
    10141 tatctggacc attcgcatga aaagcggcga tgtccgcctg atgcacaaac ggacggtcga
    10201 catggagatc gcgggtcatg aatttttcag tcccgacggg aaaaccattt atttcgactg
    10261 gcagataccg aaggggcaga ccttttatct cggggcggtg gatgtcgcca ccggcgccga
    10321 gaagaaatat gcgatcaccc gcgacgaatg gtcgatccat ttcaatgtcg cccccaatga
    10381 aaaattatat tgcggggatg gcggggatgc ggggcaggtc gcgcatgcga aggacggccg
    10441 ctggatctat cttttccgtc ccgagggcga cagcctggcc gcagagcggc tggtaaatat
    10501 gaaggatcag tattacaaac tggagccaaa cgttcatttt tcgccggatg ggaaatgggt
    10561 gatctttcgt gctaacttcg agggtaagga agaagtgtat gccgtcgaat gtaaaaagtc
    10621 ataatatcgg atgagatctc tgttgctgtt tgtcctcctg accgtccagc tgtcggtgtc
    10681 tgcccagttg caatggccgg cgatcaccgc gcaaacaaag ccgtggaccc gctggtggtg
    10741 gatgggcagc gcggtcgata aggccgatct gaccgcgaac atggaaaaat atgccgcggc
    10801 gggtctcggc ggtctcgaac ttacccctat ctatggcgtc catggttacg aaaaccaatt
    10861 cgtcgactat ctttcaccga aatggatgga cctcttcggg tatacacttg gggaggccag
    10921 gcgattggga ttgggtctgg atatgacgac cgggacagga tggccgttcg gcggaggacc
    10981 gctgatcgac tccacctatg catgccggga aatgtttcac aagttgtggg cggtgaaggg
    11041 aggagaagga ttgcctgatt ccattcgcta tcagcaggaa ggttatgtac atacggacgg
    11101 gcgcccgacc accatcgccc agctggtgga acccgtgttt gcaaataagg accgccaggc
    11161 cctcgcgctc ttccaggtgc ggttcccgca ttcgctgccg ccatactcgc tcatggcgta
    11221 ttcgggggat ggccgcgtgc tcgatctgac gaacagggtc gatgctaaag gtcatctcga
    11281 ctggaccgcc ccggctggca gcgactggac gctatatgga ctgttcctcg gctggcatgg
    11341 gaaaatggta gaacgcgccg ccccgggcgc cgaaggtaat gtcatcgatc acttctcgtc
    11401 cgtggcgctg aggaaatatt taagccggtg ggacacggca ttcgggcatc tcgatctatc
    11461 gccgctgcgc tgtttcttca acgacagtta tgaggtggac gactcgcgcg gtcagagcaa
    11521 ctggaccccc ggttttttcg acgatttccg gcatctccgg ggatacgatc ttcgtgaaca
    11581 tcttcctgcg ctgtttggaa aaggtaacag cgaggatatc gcgcgtgtca tttgcgacta
    11641 ccgggagacg atctccgatc tgttgctgca acagttcacc caaccctggc atgactgggc
    11701 gcatgcgaaa ggcgcccgca tccgcgacca ggcgcatggg tctcccgcga atatcctcga
    11761 tctttatgcg gcggccgaca tcccggagac ggaaggcagc gagatcctgc gttataaatt
    11821 cgctacttcc gccgcgcatg ttacaggcaa accactcgcc tcggccgaga cggtcacgtg
    11881 gctgaatgaa catttcctca gcagttatgg agatgtaaag accgcactcg ataattattt
    11941 cctcggcggt gtcaaccatg tcttttatca cggcactgcc tatacgccga tggccgaccc
    12001 atggccggga tggcttttct atgcctcggt gcactttact cccaatgatc ctgcctggcc
    12061 cgatgtctca acgttgaacc ggtatgtagc ccgttgccag tcctttctgc aaaagggagc
    12121 gccggacaac gatatcctgt tgtattatcc gctgtacgac agctggtccg agccggggaa
    12181 tgtcttgctc aaacactatg accggatgga tcccgaattt accggcaccg ggttcatgac
    12241 ttgtgcggac tttctgcagc atcatgggta taccttcgac tttatctcgg accggcaatt
    12301 gcagggagtg acggcgggcg ccggtggtgg gatcgttacg ggtggggtaa cctacaagac
    12361 catcctgtta cccgattgcc ggtatttgtc ggtggacgcg ttgaaaaaac tggtcgggct
    12421 tgcccgtcat ggggtgaaag tgttggtgta taagcgacta ccttatgatg taccgggttg
    12481 gggccattgg caggaacgcg agacgcagtt caaagaattg ctgtcgggga ataattttgc
    12541 tgtaggtgag gatctcgaca gtctgttgac aaccgcgaac gtccaccgtg aaaagctcat
    12601 cgatgatagt attggattct ttcgccgcag gctcggttcc ggcgaagtat attttatcgt
    12661 caataaaggt cagacggctt ttgccggtta ccttccgctg gatcgtgtgt ttcgcagcgc
    12721 cgccattttc gatcccatga cgggcgggaa aggcatggcg cgaagaagag gtgattcggt
    12781 atatgtgcaa ctaaggcgcg gtgagacctg tatcatccag accaaccccg ccgggggggc
    12841 gtcgctgttt ccctattatc tgccgatggg aggcagcatg cgcttgaccg gaagatggca
    12901 tctcgaattt ctgtcgggcg gacccgtcat tccgcatgac acgacggttg gtatgctggg
    12961 ctcatggacc gatctgggag acgaggcgat gcagaatttt tcaggtgtcg gaaaatattc
    13021 gatcgatttc gaccggcccg cgacgggcgt tcaggcctgg cttctccaat tgggcaaggt
    13081 atacaggacg gtcgaggtct ttctcaacgg tcacaagctg accactctgc tgggcccgga
    13141 ttatgaagtc gtcattccct cttcggctat gcatcaaaga aataccctcg aaataaaagt
    13201 ggccaacacc atggtcaacc gtatcgaata catggatcgc cggcatattc tttggaagaa
    13261 attctacaat tacaatttcc cggcgcacga gcgcgccaac cggggtgcgg atggtctttt
    13321 cgatacgtcc aaatgggctc ctatggactc cggactatcg ggtccggtga cgcttttgcc
    13381 tttgcggcta ttgccatgac aagaatcgtc ctttatatgg tgtatgcact aatactggcg
    13441 ccgggtttcg tcccggccca ggtccgtttg gcagcggcga agggtggtcc gggcaagggt
    13501 cattcggtgt cggcggaggg tggtccggcg gatcacatgg tgcggtatca tcctgaaggt
    13561 ggcgatttcg tgatcgtcaa cgggacgcga agattcaacc gcgcgctgta cggcacccac
    13621 accgcctttc gtgtcgaggc cggcgacctt cccgaatttg ccttttacat gcccgggatg
    13681 ggcggtaatc tgcatttcga gctggaaggg cgcccgctga tccggtctca atacatcaag
    13741 gccatctacc gtcccggatc gatgttgtac gaaatacgcg acccggcgct cggagccaaa
    13801 ggggtactat cgatgacggt gctggcgctg gccgatgccg aagggatgat cattcgtatc
    13861 agcggccgga atgcgggacg gcatttgggc gtggtcttcg gcggcgcatc gggtaaaaaa
    13921 ttctcgcgcg acggagatat cggcgccgac cctgaatcgt cgttctacct ggatagcgca
    13981 ggttgtcggg gtgatcatta tgtagtgagg ggggacggtt ggtcgttgga gtatggggca
    14041 ggtaaaaagc tggaaggtat ttttcctccc ggctctgggt tgcgggtgga tgcggtgccg
    14101 gcgttgcatg ggcaactgtc actgggggat acctgttatc tgctgatacg caatcctgct
    14161 tcggcatcgg cgtctttgcc ctcctatagc gaactgccgg ctttcttcga ctccgccgag
    14221 accgtacgcg aacgtcttgc cggtcgcgta gtgttgcata cgcccgatcc atggatcaac
    14281 acccttggcg gtgcattggc cgtcgcagcg gacgccatct gggaagaccc atcatatctg
    14341 cacggcgccg tcgcctggag acagcgtctt cccgcgtggc gggggcctta tgtcgccgat
    14401 ccgctgggtt ggcatgatcg cgcccgccgg catttttcaa gctatgctct ttcacaactg
    14461 accgagcctg caactgcacc ggtagtggcc gatacggcgc tgcatctcgc gcgtcagctg
    14521 gagcgtctgg gtacttccct attcagcagc gggtacatct gccgtaaccc cggcggtgat
    14581 cttcgtccgc atcactatga tatgaacctc gtttttatcg accagctatt gacgcatttt
    14641 tactggacag gggataccgc gtatgttcgg caaatgtggc cggtgctgac gcgtcatctg
    14701 gcctgggaga aacggaactt cgatagcgac ggcgacggtc tctatgacgc ctatgcctgt
    14761 atctgggcaa gcgatgcgct ggaatacggc ggcggaggag tgacacattc ttcggcttac
    14821 aattatcggg cgaataagct tgctgccgac ttggcgcggg tgatcgggga agacccggcg
    14881 ccctatgctg ccgaggctgc gcatattcta aacgctatca atagccgctt gtggatgacg
    14941 gagaaagggt ggtatgcgga gtatatcgat tgggcgggag gccacgggac gccggtcctg
    15001 catccgtcgg cggggttatg gacgatctat catgccatcg acgaaggggt cgccgatccc
    15061 gttcaggcct gggaatcgct gaaatacatc gacagatata taccgcatat ccctatccgt
    15121 gcccgggggc tgggcgacac gacgctatat ttattgagca ccaccaattg gcagccatat
    15181 acctggtcgc tgaacaatgt tgcgctggcg gagagtttgc atacctcgct ggcttactgg
    15241 cagggagggc gaccggatga agcgttccgg atatggaaga gcgcactggt ggaaagcatg
    15301 tacatgggtt cgagccccgg gaatttcgaa cagctgtcct catacgatgt cgcgcgcggc
    15361 gagctctacc gggattttgg agatggcatc ggcatggcgg cgcggagcct ggtggaagga
    15421 ttgttcggca tatcgccgga tgctatacac gatacgctca cgatcgctcc gggatttccc
    15481 ggggagtgga caaaggcttc actgcatata ccggatatca gtgtggagtt ccggcgtcag
    15541 ggccggacgg atgaatatca tatttttccg aatttcccgc ggcatatgaa cctgcgtttg
    15601 cggcttcatg ctcaggcgga gacgatcaga cgtgtggtag tcaacgggaa gccggcttcg
    15661 tggaagaaga tcgttgaaga ggtgggcgtt ccggtgatgg aggtggatgt tccgccggcc
    15721 acggaatacc atgtttccgt gtcatgggga ggcgggccgg cgtttggaat aggaatagct
    15781 ttgggcgggc cgaggttaag aacatacacc gggggatcgg atttgccggc caaaggcgaa
    15841 cacgcggata cggtttcgct gacgggatac ttcaatgatg atgtgacgca tatctttcaa
    15901 caacaatact tgtcgccacg accaaagagt ccgaccttac agctgccgac gcagggcatc
    15961 ggcaattggt gttacccgct ggtgaatgcc gatatcgatg atgcgggact aagggcatcg
    16021 ggtggagagg tccggctgcc gggcgggata ccgttcgcta ctcccgccag cggtcacaat
    16081 attgttttca cctctttgtg ggacaactac ccggattcgg tatcggtacg gctgggcggg
    16141 caggcaaaac atgcctggtt cctgatggcg ggatccacca accccatgca aagccagatt
    16201 accaacggtg ttatcgatgt gtattatacc gacggcacct ccgacagact cgagctggtg
    16261 aatccttcca actggtggcc catcgaagaa gattatctcg aagacggtcc ggccttcacc
    16321 accggagctc cacatccctg gcgggtgtat ctaaagacgg gcgtcgtcag caatcaaccc
    16381 ggcaaatgga cgacgatcaa aggtcttacc aataccgcca tcgagggggg cgcggctacc
    16441 gttgccgata tgactttaca gcccgacaag acactggatc atctcgtgtt gcggacgacg
    16501 gcgcatgacg tagtggtcgg actgatgagt atcacgttaa ttaaaaaaaa tgaatgatga
    16561 aatatctatt gatcttgtta ctgatgccct ggtccggccc ggagcggggg ggcaaagcag
    16621 gtgcggaccc ggactggacg actaaagtcg gggcgcatga attcccacac ggcaaggcga
    16681 tggtgactgt cacgggcgct gcgaaagacg gcagtaagct tgcaactcat gccatccaat
    16741 cggctatcga cgactgcgcg gccaaaggcg gaggcgtcgt cgttttcgcc ccgggtatct
    16801 atcagacggg gagtatcttt ctgaaagcga atgtcgagct tcgtatcgac aagggcgtag
    16861 agcttatcgg cagtcaacat ttcgaagatt atcccgagat cgacacccgg atcgccggta
    16921 tcgagatgcg atggccggct gcgctgctga atatcatcgg ggtcaaaaat gcggctgtca
    16981 ccggcgaggg caaggtcgac gcgcgcggaa agttctgctg ggacaaatat tgggccatgc
    17041 ggaaagaata cgatgccaaa gggctgcgct ggatcgtgga ttatgacgca cagcgggtaa
    17101 ggaccttgct catccagcga tgcggcgatg tgacgataaa gggggtaacc ttcgtggatg
    17161 cggggttctg gacggtgcag gtcttatatt cggagcatgt gacggttgat ggcatcgtcg
    17221 tacgcaacaa tgaaggcggc catggaccca gcaccgacgg tgtagacatc gatagctcgt
    17281 cctgggtgct ggtgcaaaat tgcgacatcg attgtaatga tgatgatttc tgtctgaaag
    17341 ccggtaggga ctgggacggt cttcgtgtca acaaacctac tgagtatgtc gtcatccggc
    17401 attgcgtcgc acggaaagga ggcggtttgc tgaccatcgg cagcgaaaca tccggcggga
    17461 tacggcatgt gctcgcaacc gacctgacgg ccaggggcac gggtaacggg tttcatatca
    17521 agtcggcgac tacgcgaggt ggaacggtag aagacatcca tgtgaagaat attacgatgg
    17581 acagcgtggg taatgccatc ctcttcacca tgaactggaa ccc
//
LOCUS       DASYSV010000097        13641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_276166, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000097 DASYSV010000000
VERSION     DASYSV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13641
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276166"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VGQ51_02965"
     CDS             <1..355
                     /locus_tag="VGQ51_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035549.1"
                     /translation="FVTFTNMAKHKNTTPHDPVKDAQREAEKDIHLDPEFAEGDPTDD
                     LDEGELARRDNSDEEGLNALDKKRPRGGNAGTGHAGTGPAAKGGNAPHPAKGGGAPHS
                     GKGNNAPHSGKGSEK"
     gene            complement(328..1956)
                     /locus_tag="VGQ51_02970"
     CDS             complement(328..1956)
                     /locus_tag="VGQ51_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV9035550.1"
                     /translation="MEEALPQANGKFEAVNKQAMLAAIIESSVDAIISKTLGGYITSW
                     NQAAERMFGYTENEVIGRHISLLIPPDKLDEEDMIIGRIRSGLRVEHFQTERLTKTGD
                     IIPISLTISPIKDSDGRIIGASKIARDISDQVRAQQELQTHAHIQELLLSAGTLLSQN
                     LELRSILQKVTDITTQLSGAEFGSFFYNMTDANGETYMLYTLSGVPKDHFKDFPMPRN
                     TPLFQPTFSGEAIVRSDNIREDPRYGRNAPYNGMPDGHLPIISYLAVPVSSPSGTATL
                     GGLFFGHSKPAMFREEHEKLVAGIAAQAGAAIENAKLYEEIRQLNTRKDEFIGVAGHE
                     LRTPLTTVKGYLQLLEDQMPEGLSKDFTVKAIRQVNKLNRLVADLLDVTKIQAGKLEY
                     TMMPCFMLPLIRESLDTVGQMHTTHRIVAELPDEDIVVTADSTRIEQVLINFLTNAIK
                     YSPDASEIGLSVMVKEARVIVSVQDWGMGIPKEHQAHIFNRYYRAHSDRIVSGMGIGL
                     YIAKEIIDRHGGTIGVDSQEGVGSVFYFSLPLPE"
     gene            2120..2572
                     /locus_tag="VGQ51_02975"
     CDS             2120..2572
                     /locus_tag="VGQ51_02975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEV9035551.1"
                     /translation="MPLLEKQLVVRRSTLKGAGKGLFTTKAIPVGKKIVEYKGEITTW
                     KEVNDSKGFNGYIYYINRHHVIDAATAKEALARYANDAKGIVRTKGLLNNCTYQIEGL
                     RVFIYSMRDIPAGSELFVGYGKEYWDIIRENRRLDSAGSRPPKRTKVH"
     gene            complement(2577..2897)
                     /locus_tag="VGQ51_02980"
     CDS             complement(2577..2897)
                     /locus_tag="VGQ51_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035552.1"
                     /translation="MISNSPYDPIEPTAFLGSGYHTVITLRIRPGAPVHTKRTVLSIL
                     IAVPIAYYAMRQWLQGYSYRAPLSVWIFVSAGVGILAVTLATVSYQSIKAARMNPVKS
                     LRSE"
     gene            3088..3687
                     /locus_tag="VGQ51_02985"
     CDS             3088..3687
                     /locus_tag="VGQ51_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV9035553.1"
                     /translation="MQKANTAKKTAAKGAHAPAKGNAHARSTAGGAEDSKLKEFFVDE
                     LKDILWAEQKLVKTLPKLQKAATSGELQDAFESHLEETKTHVDRLEQVFKLLGEKATA
                     KKCDAMEGITEEGSSVIEDTDEGTATRDVALIMAGQKAEHYEIATYGGLIQIARTLGQ
                     EDVADLLEETLEEEKQADQKLTEIAEAGVNSQAGNEPRD"
     gene            complement(4283..6826)
                     /locus_tag="VGQ51_02990"
     CDS             complement(4283..6826)
                     /locus_tag="VGQ51_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEV9035554.1"
                     /translation="MIPIKNAIWMAALAVVTTVPAMAQRRRANPVPTDTTKPRIPGFP
                     GFNLPKQGPKPYKDVITDKAITHTGLFTVHQIEDKWYFEIADSMMEKEFMAITRFSKT
                     AAGGGIYGGELANQQTMEWEKGPSHTLFLRVVTLVSMADSTNKIYKAVTNSSVNPIAA
                     AFDIKAYGKDSSSVVIDVTDFFKGDNQVVSIPQHVKSRMRLGGIASDRSFIGHIHTYP
                     INTEVRTVKTYSSGGGMPSPFGLSITTPTDAAGAVTLELNTSFILLPAVPMAKRLFDP
                     RVGYFADDYTVYSDAQQKAENQEFIVRWRLEPKPDDRDKWLRGELVEPKKPIVYYIDP
                     ATPRRWVPYLMQGIDDWQQAFEKAGFKNAIQAREWPDGDSTMSPEDARYSVLRYFASD
                     IENAYGPNVHDPRSGEIIESHIGWYHNIMEILHDWYMIQAAAVDPRARTMHLDDSLMG
                     QLIRFVSSHEVGHTLGLRHNMGSSSTVPVERLRDRSWLDIHGHTPSIMDYARFNYVAQ
                     PQDSLPERDLFPRIGEYDRWAIRWGYGYAGDGDPKKESKVLNKWVIDSLRTNPRLWFG
                     GEGFNGDPRAQMEDLGDNSMKAGEYGIKNLQRILPSLPVWTREEADKYDNLEEMYKQL
                     VAQFNRYMGHVLRNVGGVYETFHSVEQPGDVYQPTPKAVQREAVRFLDAQLFETPRWL
                     QDTAILNKISNPDGGDPVAPVQASVLSSLLSAGRLNRLLQSAERYGDARVYTVEDLLG
                     DVRTDIWKELATHRTIDVYRRGLQKTYVESLIMLANPASPSSSGIPGIILFFGPNTKN
                     TDLPSIAKAELSALRARIAAAIPLTADRLSKYHLQDLADRIRRALEPKG"
     gene            6887..7555
                     /locus_tag="VGQ51_02995"
     CDS             6887..7555
                     /locus_tag="VGQ51_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035555.1"
                     /translation="MKIIPFGLAPVLGCLALVLGFAAAAQTPSSSSTSGGQTLSLNGE
                     EHAGLVTGRHHSLIAYAGKDHSFTLDIGAYPAKPSDIPGFISIDKQIVQVTLVPVSGS
                     AGLNALTTDKEKEMLLKYMNYELEFYKKKLKQHYSHLQTEWVSLNGRLFLLWYFDMPE
                     NYKLVSRQVYVSTLFYNRVMDLNAPVFKSDDWGRARAILVKLAGTMRTYDRRLDLEGL
                     RKKL"
     gene            complement(7564..7863)
                     /locus_tag="VGQ51_03000"
     CDS             complement(7564..7863)
                     /locus_tag="VGQ51_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV9035556.1"
                     /translation="MAKKKTVTSFENHLIKRYGRRGTEKRTEFEIKAKAFMIGELIRE
                     ERRLANMTQEQLADRIGAKKSFISRIENGHCDIQLSTLFRLLEFGLGKKVELVVH"
     gene            8176..9408
                     /locus_tag="VGQ51_03005"
     CDS             8176..9408
                     /locus_tag="VGQ51_03005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV9035557.1"
                     /translation="MKPFLLFPLLLIAVCPVSAQSKPSKPATYFPPKGQWERRAPGQM
                     GLDSLQLQAAIRFARDNESRAPRDQELAAAESFGKNEPFDAGVGPFKERGDPTGVIIY
                     KGYIVAEWGDPSRVDMTHSVTKSFLSTVIGVAVDQGLIGSVMDTVAGYVPPIEVYDPA
                     APQRSPEDIGKPQLLYPFESPHNKVLTWDVMLRQTSDWEGVLWGKPDWADRPEGPANE
                     WTTRKRHAPGTFWKYNDVRVNALALAATSVWRMPLPQVLKTYIMDPIGASNTWRWNGY
                     RNSWIVLDGQPVQSVSGGGHWGGGMFIDAYDMARFGLLTLHRGNWNGRQLISEQWVKQ
                     ALTPTSVQPDYGYMNWFLNTNQKLLPSAPASAFVHIGNGTNFIYVDPQHDLVAVVRWI
                     ANPAMDGMVKQILLALGK"
     gene            9414..10037
                     /locus_tag="VGQ51_03010"
     CDS             9414..10037
                     /locus_tag="VGQ51_03010"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV9035558.1"
                     /translation="MELAKTYRIDTERLLIRCYEPADAPKLHQAITGSLEHLRPWVPW
                     ARQEPREQEWMESFIRMCRGQFDLGQDAIYGIFDGRTEQLIGGTGLHNRIGKDAREIG
                     YWIDARHTHHGYATEAVCALIRVGFGIERLSRIEIRCSPENIPSRRIPQRLGFRHEMT
                     LKGNATDLKGQPADTMVWCMTKSDYDLSPLRATTLRAIDFMGREIAL"
     gene            complement(10034..11251)
                     /locus_tag="VGQ51_03015"
     CDS             complement(10034..11251)
                     /locus_tag="VGQ51_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HEV9035559.1"
                     /translation="MRILVSSLICLLTVAVTAQEHHQLQPKPALLRLIDGSLRQAVNQ
                     YKVLMGRVPPDRLPKTWYANTGRLETSDPGWWTSGFYPGTLFELYSFSHDTTLLREAT
                     DRLRLLAPEQYNKGTHDLGFMLYCSYGNALRVADRPGYKEILLNAARSLSTRFNPTVG
                     CIKSWDSKPWHYPVIIDNMMNLELLFWASSVSGDSSFYHIAVTHANTTMRNHFRPDYS
                     SFHVVDYDSATGAIIAKKTAQGYSDSSAWSRGQSWGLYGYTMCYRWTHDARYLQQARH
                     IARFILTHLPADKIPYWDYNAPGIPAGTSAQTPGPLRDASAAAVLASALIELGGDVPA
                     SEGRGYLDVAEQIIVTLSSNAYKAIVGSNGGFILLHSVGNLPGRSEIDVPLTYADYYF
                     VEAMLRYKQLAAH"
     gene            11278..>13641
                     /locus_tag="VGQ51_03020"
     CDS             11278..>13641
                     /locus_tag="VGQ51_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEV9035560.1"
                     /translation="MEEKLKILGGGLEGELYFDKTMRTLYATDASAYREMPLAVAIPK
                     TTDDIKRLIAFAKTNHTSLIPRTAGTSLAGQVVGKGIVVDVSHYFTRILELNADERWV
                     RVQPGVIRDDLNLFLRPHGLLFGPETSTANRAMIGGMVGNNSCGSNSIVYRSTREHLL
                     EVKAILSDGTEATFTALGPDDFHAKCEGNGLEAEIYRSVRQMLSNYDNQVAIRENFPK
                     KSIERRNTGYPLDLLVETAPFTAGGPDFNFCSLLAGSEGTLAFLTEIKLDLSPLPPKE
                     TGLLCVHFHNIDESLRANLIALRYHPTASELIDHFILECTKDNIEQSKNRFFVQGDPG
                     AILVIEFKKDTREEIAAIAAAAEADMRAAGLGYHFPLLFGADTGRIWSLRKAGLGLLA
                     NLPGDDKAVPVIEDTAVDVQDLPEYIRDFNEILKKYGLYSVHYAHAGSGELHLRPIIN
                     LKTAEGNRLFRVIADEIATLVKKYRGSLSGEHGDGRLRGEFIPRMVGERNYSLFKSIK
                     NTWDPAGIFNPGKIVDTPPMNEHLRYEPGQQTPPFPTVFRFHQQDILQHAEQCNGSGD
                     CRKTHLSGGTMCPSFMATRNEKDTTRARANILREFLTRDDSPNRYDHKEIYEVMDLCL
                     SCKGCKSECPSNVDVAKLKAEFLQHYYDANGVPLRTRLIAGFTRSAQLGSMAPGVYNL
                     FMRSRLLKRLIGFAPDRSMPSLHKTTLRKWWKGHRREPASRRVYLFCDEFTNYNDVEI
                     GIKAILLLERLGYEVIIPDHIESGRTWLSKGLVRKAKAIAGENIRLLS"
BASE COUNT         3153 a         3715 c         3868 g         2905 t
ORIGIN      
        1 atttgtgacg ttcaccaata tggctaagca caaaaatacc acgccgcacg accctgtaaa
       61 ggacgcgcag cgcgaggcgg aaaaagatat tcatctcgac cccgaattcg ccgaaggcga
      121 tcccaccgac gatcttgacg aaggcgaatt ggcccgaagg gacaactccg atgaagaagg
      181 gctgaacgcg ctggacaaga aacgcccgcg cgggggcaat gcgggcacgg gtcatgcggg
      241 cacgggtccg gcggccaaag gcggcaacgc tcctcaccct gccaaaggcg gcggtgctcc
      301 tcactccggc aaaggcaaca acgcccctca ctccggcaaa ggcagcgaaa aatagaagac
      361 agaaccaaca ccttcctggc tgtccacgcc gatcgtgccg ccatgtctgt ctatgatctc
      421 tttggcaatg tataagccga tgcccatacc gctgacaatg cggtccgaat gggcccgata
      481 atacctgttg aagatatggg cctggtgttc tttcgggata cccattcccc aatcctgcac
      541 gctgacgatc acccgagctt cctttaccat gaccgaaaga ccgatctcac tggcatccgg
      601 cgaatacttg atggcattcg taaggaaatt gatcaatacc tgttcgatcc tggtgctgtc
      661 ggcggtgacg acgatgtcct catccggcaa ttcggccacg atccggtgcg tggtatgcat
      721 ttgtcctacg gtatccaggc tttctcttat cagcggaagc ataaaacagg gcatcatggt
      781 atattcgagc ttgcccgcct ggattttggt gacgtcgagc agatcggcga ccagcctgtt
      841 cagtttgttc acctgccgga tcgcctttac cgtaaaatcc ttcgacaggc cttccggcat
      901 ctggtcttcc agcaactgga gatacccctt gacggtagtg aggggcgttc gcagctcgtg
      961 acccgctacg ccgatgaatt cgtccttccg ggtattcaac tgccggatct cctcgtaaag
     1021 ctttgcattc tcgatggccg cacccgcctg tgcggcgatg cctgcaacca gcttttcgtg
     1081 ttcctccctg aacatggccg gtttggaatg accgaagaac aatcctccga gcgttgccgt
     1141 gcctgacggc gagctgacgg gcaccgcgag atagctgatg atgggcagat gcccgtcggg
     1201 cattccgtta tacggggcat tccggccata acgcggatcc tcgcggatat tgtccgagcg
     1261 cacgatggct tcgccgctga aagtcggttg gaataaaggt gtattgcgcg gcatggggaa
     1321 atccttaaag tgatccttcg gcacgcccga taaggtatac aacatgtagg tttccccgtt
     1381 ggcatcggtc atgttgtaaa agaaagagcc gaattccgcg cccgacagct gggtcgtgat
     1441 atcggtcact ttctgaagaa tggatcgcag ctccaggttt tgcgatagca gggtgcccgc
     1501 cgataacaac agctcctgga tatgcgcatg ggtctgtagc tcctgctgcg ccctgacctg
     1561 gtccgagatg tcccttgcga tcttggaggc gccgatgatc cggccgtcgc tgtcctttat
     1621 tggagaaatg gtcagcgata tcgggatgat atccccggtc ttggtgagcc gctcggtctg
     1681 aaagtgctct acgcgcagac cgctgcgtat ccggccgatg atcatatctt cttcatccag
     1741 cttgtccggc gggatcagca gcgagatatg ccgtccgatg acctcatttt cggtataccc
     1801 gaacattcgc tccgccgcct ggttccaact ggtgatataa ccccccagcg ttttgctgat
     1861 gatggcatct acggacgact cgatgatggc ggcaagcatc gcctgcttgt tcacggcttc
     1921 gaactttccg ttcgcttgcg gtagggcttc ctccataacc gcaatttacg tgaatggccg
     1981 aagaatcccg cgctccccgg gagattttca tacggctggc tggctcggga attgctgccc
     2041 gaggctgata gaagaggtcc cggtcatcca gccataatac ctatattgcg catcctaaaa
     2101 ggctatacaa attcgcatca tgccgttact cgagaaacag ctcgtcgttc gacgatccac
     2161 cctgaaaggc gccggaaagg gcctcttcac taccaaagcc attccagtcg gaaagaagat
     2221 cgtggaatac aagggggaga tcaccacgtg gaaggaagtg aatgatagca aagggttcaa
     2281 cggatatatc tattatatca atcgccacca tgtcatagat gcggcgacag caaaggaggc
     2341 gctggcccgc tatgcaaacg atgcgaaagg tatcgtccgc accaaaggtc tcctgaacaa
     2401 ttgcacctat cagatagaag gattgcgcgt attcatctat tccatgcggg acatccccgc
     2461 gggaagcgag ctgttcgtgg gttatggcaa ggaatattgg gacatcatcc gcgagaaccg
     2521 gcgtctcgac tccgccggat cccgaccgcc aaaacgcaca aaggtccact gaaagatcat
     2581 tcggacctta aactcttcac aggattcatc cgtgcggctt taatggactg gtagctgacg
     2641 gtcgccagtg ttaccgcaag gatgccgacg ccggcggaga cgaaaatcca cacggacagc
     2701 ggggcccggt acgaataacc ttgcagccat tggcgcatgg cgtagtacgc gatgggtacg
     2761 gcgatgagga tggacaggac cgttcgcttc gtatgaaccg gggcgccggg ccggatccgg
     2821 agggtgatca cggtgtggta gccgcttccg aggaaggcgg tgggctcgat ggggtcgtag
     2881 ggggaattgg agatcatgtc ctttcgtaca ttttgtgtgc cagtttatgc taaatgccct
     2941 cgcggaatgg ctgggcgtca gcgggccgat tgcgatggtc atggctcatc atgtggaaaa
     3001 acctgtacaa attgtcggca attcagaccg ggcttgcgag tgacccggat ggcatgattt
     3061 tcctttgggt aatacaaaac caattttatg cagaaagcaa ataccgcaaa aaaaaccgcg
     3121 gccaaaggcg cccatgcccc tgcgaaaggg aatgcccatg cccgaagcac cgccggaggg
     3181 gctgaagatt cgaagctgaa agaatttttt gtagacgagc ttaaggatat cctttgggcg
     3241 gaacaaaagc tcgtcaagac gctgccaaag cttcagaaag cggcgacttc gggggaattg
     3301 caggacgcgt tcgaaagcca ccttgaagaa acaaaaacgc atgtagaccg gctcgaacag
     3361 gtgttcaagc tactgggaga aaaggccacg gcaaagaagt gcgatgccat ggaagggatc
     3421 accgaagaag gaagttccgt gatcgaagat accgatgaag gtacggcaac gcgcgacgtg
     3481 gcgctgatca tggccggtca aaaggccgag cattacgaga tcgcaaccta tggcggcctt
     3541 atccagattg cgcggacact cggccaggag gatgtcgccg acttgttgga ggagacgctc
     3601 gaggaggaaa aacaggccga tcagaagctg acggagattg ccgaggcggg tgtcaatagt
     3661 caagccggta atgagccgag agactaggaa tatctttgga taccatctca tgtgcgcggg
     3721 ccccttcggg gcccgcgctt ttctaatcga ggaccaggtg ccgcaagttt tttgcctgag
     3781 ttgatggagg gtttggagca gaaggtggat gagagtttgt gaattttttt ccgttatgac
     3841 caaagtattg aagaactgtg tagtcagcgg ggcagtagcg ttgtggtcaa ggccgagatt
     3901 acatctgtcg taaatggtgt caagagcggg cgaaacggcg acattttcgt aaacggagtt
     3961 cttacagaaa tagagtcgat tagctcagta cgcggcgatt tgtctaaagc cataaagatt
     4021 aggctttcgg acgcttcgtc tcaagcgaaa aatgtaataa tagatttaac cgaacaacaa
     4081 ggcgcgacct tggaagagga ttccgtgcaa gtttaatcaa ttccgaaaaa tcaaatgaca
     4141 gatcgtataa aatatacacc aatgctggcg aagtctttaa ggagagcgtc agggatcgaa
     4201 gtgaaaagcc cacaggccaa ataggccgag gacttgtaac aaagagcccg aaccgaacgg
     4261 ggacgcccca aaaaaaacat cctcacccct tcggctccag cgcccgcctg atcctgtccg
     4321 ccagatcctg caaatgatat ttacttagcc ggtccgctgt cagcggaatg gccgcggcga
     4381 tccgcgcccg cagggccgaa agctcggcct tcgcaatcga aggaagatcg gtattcttcg
     4441 tgttgggtcc gaaaaacagg atgatacccg ggatgccgga ggaggacggc gaagcgggat
     4501 tggccagcat gatcagcgat tcgacatagg tcttttgcag gccacggcga tagacatcga
     4561 tggtacgatg ggtggccagc tctttccaga tatcggtccg gacgtcaccg agcaggtcct
     4621 ccaccgtata aacgcgggcg tcgccatatc gttcggcaga ctgcagcagg cggttgagac
     4681 ggccggcgga aagaagactg ctcaggacac tggcctggac gggcgcgacg ggatcgccgc
     4741 cgtcggggtt gctgatctta ttcaggatcg cagtgtcctg gagccaccgc ggcgtttcga
     4801 acaactgggc atcgagaaaa cggacggcct cgcgttgaac agccttcggc gtaggctggt
     4861 agacatcacc aggctgctcg acgctatgaa aggtctcata gacgccaccg acattccgga
     4921 ggacatgtcc catataccga ttgaactgcg ccacgagctg tttgtacatc tcttcgagat
     4981 tgtcgtattt gtccgcctct tcgcgcgtcc agaccggcaa cgacggtagt atccgctgta
     5041 aatttttgat gccatattcg ccggccttca tactgttgtc gccgagatcc tccatctgcg
     5101 cacgcgggtc gccgttgaac ccctcccctc cgaaccacag ccggggattg gtccgcagac
     5161 tatcgatcac ccatttgttg aggaccttcg attctttctt cggatcgcca tcaccggcat
     5221 agccataccc ccaccggatg gcccatcggt cgtactcccc gatacggggg aacaggtccc
     5281 gctcggggag gctgtcctgc ggctgtgcca catagttgaa ccgggcataa tccattatcg
     5341 aaggtgtatg accgtggata tccagccagc tcctgtcgcg gagcctctcg accggcacag
     5401 tgctgctgct gcccatgttg tgacgcaatc ccagggtatg ccccacttca tgcgaggaga
     5461 caaaccgtat cagctgaccc atcaggctgt catcgagatg catcgtcctg gcgcgcgggt
     5521 cgaccgcagc cgcctggatc atataccagt cgtgcaggat ctccatgatg ttatgatacc
     5581 agccgatatg actctcgatg atctcaccgc tgcggggatc gtgcacgttc gggccatacg
     5641 cattttctat atccgacgca aaataacgga gcaccgaata ccgggcatcc tcgggactca
     5701 tcgtactgtc accatcgggc cactcgcgcg cctgtatggc atttttgaac cccgcctttt
     5761 cgaaggcctg ctgccaatcg tcgataccct gcatcagata ggggacccac cggcgcggcg
     5821 tggcagggtc gatataatat acgatcggtt ttttcggctc taccagctct ccacgcaacc
     5881 atttgtcccg gtcgtccggc ttcggctcca gccgccagcg tacgatgaac tcctggttct
     5941 ccgccttctg ctgtgcgtcg gaatagaccg tatagtcgtc ggcaaaatat ccgactcgcg
     6001 gatcgaacaa ccgcttggcc atggggacgg ccggcaaaag gataaaagaa gtatttagct
     6061 ccagcgtgac ggcaccggcc gcatccgtcg gggtggtaat ggacagaccg aagggcgacg
     6121 gcatcccgcc gccggaagaa tatgtcttaa cggtacggac ttcggtattg atgggatagg
     6181 tatggatatg cccgatgaag gaccggtcgg aggcgatacc gccgagccgc atccggctct
     6241 tgacatgctg cggtatgctg accacctggt tgtccccttt gaaaaaatcc gtcacgtcga
     6301 tgaccacgct ggaggaatct ttgccatagg ccttgatatc gaaggccgcg gcgatggggt
     6361 tgacgctgga attggtgacg gccttgtata tcttattggt cgagtcggcc atgctgacga
     6421 gggtgaccac ccggaggaat aaagtgtgtg aaggaccctt ttcccattcc atcgtttgct
     6481 gattggcgag ctcgccgcca tagatgccgc caccggcagc ggtcttgctg aaccgcgtga
     6541 tcgccatgaa ttccttctcc atcatcgaat ccgcgatctc gaaataccat ttatcctcta
     6601 tttggtggac ggtgaaaaga ccggtatgtg taatggcctt gtcggtgatc acgtccttgt
     6661 agggctttgg cccttgtttg ggcaggttga aaccggggaa tccggggatc cggggtttgg
     6721 tagtatcggt ggggacgggg ttagcgcgtc ttctttgagc cattgcagga actgtcgtca
     6781 ctacagccag ggccgccatc caaatggcat tcttgattgg gatcatatga gggcggtaaa
     6841 actatacgat ttttccgttt aaattcgtta aagagccaaa aaattgatga aaatcatccc
     6901 tttcggtctc gcgccggtgc tcggttgtct cgcgctggta ctcggtttcg ccgcggccgc
     6961 tcaaacccct tcatcttcat ccacctcagg cggtcaaacc ctttcgctca acggggaaga
     7021 acacgccggt ctggttaccg gacggcacca cagcctgatc gcctatgcag gcaaggacca
     7081 ctcctttacg ctggatatcg gggcctatcc cgcaaaacct tccgatatcc cgggatttat
     7141 cagcatcgac aaacagatcg tccaggtcac gctggtgccc gtttcgggat ccgccgggtt
     7201 gaacgcgctg accaccgata aagaaaaaga gatgctattg aaatacatga actatgagct
     7261 ggaattctat aagaaaaaac tcaaacaaca ttacagccat ctccagacgg aatgggtcag
     7321 cctcaacggc cggcttttcc tgctgtggta tttcgatatg cccgagaact ataagctcgt
     7381 aagccggcag gtttatgtgt ctacgctctt ttacaaccgg gtgatggacc tgaatgcgcc
     7441 ggtgttcaaa tcggatgact ggggcagggc gcgggcgata ttggtgaagc tggctgggac
     7501 gatgaggacg tatgatcgac gattggattt ggaggggttg aggaaaaaat tgtaaccaat
     7561 cgatcagtgc acgaccaatt cgaccttttt tcccaggccg aattcaagaa ggcggaaaag
     7621 agtagaaagc tgaatatcgc aatgcccatt ctcgatccgc gagataaagc tttttttggc
     7681 gccgatcctg tcggccaatt gctcctgggt catattggca agacgccttt cctcccggat
     7741 gagttcaccg atcatgaagg ctttcgcttt tatctcgaat tccgtccgct tttcggtgcc
     7801 ccgcctgcca tagcgtttga ttaggtggtt ttcgaatgaa gtaaccgtct tcttttttgc
     7861 catgggattt atttgccttt atttataaaa tattccttca taagcttaac agccatcttg
     7921 atctcatccc gaggcgtctt catcgtctta atcgatcttc tctttcacct tctccggctg
     7981 ccggtcgaaa aactcgtgaa aataatgccg gtaaaacatc acttctctaa tcctttccat
     8041 acaaagttaa cttaaaatgg aacaatatca aaaattatta tgggagggca aatttatcat
     8101 ccctacttcg aagcggcaga aaaaaatgag tctgcaagcc ggccgcggcc tgcccaaaac
     8161 ttgtacctta atggtatgaa gccctttctc ctcttcccat tactgctgat cgccgtctgc
     8221 cccgttagcg cccaatccaa accctctaaa ccagccacct attttccccc aaaaggccaa
     8281 tgggaacgcc gcgcgccggg gcaaatgggg ctcgattcac tgcaattgca ggccgccatc
     8341 cggtttgcca gggacaacga gagccgcgct ccgcgcgatc aggagctcgc agcggcggag
     8401 agcttcggaa agaatgaacc gttcgacgcg ggcgtcggcc ccttcaagga acgcggcgat
     8461 ccgacgggtg tcatcatata caagggatat atcgtcgccg agtggggcga tccatcgcgg
     8521 gtcgacatga cccatagcgt gacgaagagc tttctttcga cggtgatcgg cgtggccgtc
     8581 gaccagggtc ttatcggtag tgtaatggat acggtcgccg ggtatgtgcc gccgatagag
     8641 gtgtatgatc cggcggctcc gcagcggtcg ccggaagaca tcggcaaacc gcagctgctc
     8701 tatccgttcg aaagcccgca taacaaagtc ctcacctggg acgtcatgct gcgccagacc
     8761 agcgattggg aaggcgtcct ctggggtaag cccgattggg cggatcgccc cgagggaccc
     8821 gctaacgaat ggaccacccg caaacgtcac gcgcccggca ctttctggaa atacaacgat
     8881 gttcgcgtca atgcgctggc gctggcggct acgagtgtgt ggcgtatgcc attgccgcag
     8941 gtgctgaaga cgtatatcat ggatcccatc ggcgccagca atacgtggag gtggaacggc
     9001 tacagaaatt cctggatcgt cctcgacgga caacccgtac agtccgtcag cggcggcggt
     9061 cactggggag gcggtatgtt catcgatgcg tacgatatgg cgaggttcgg gctgctcacg
     9121 ctgcatcgcg gcaattggaa tggccgtcag ctcatctccg aacaatgggt aaaacaggcg
     9181 ctgacaccta cttccgtcca gccggattac ggttatatga actggttcct caacaccaat
     9241 caaaaacttc tgcccagcgc gcctgcgtcc gccttcgtac acatcggcaa cggcaccaat
     9301 ttcatctatg tcgatccgca gcatgacctg gtggcggtgg tacgctggat cgccaatccg
     9361 gcgatggacg ggatggtaaa acagatctta ctcgcactgg gaaaatgaat aacatggaac
     9421 tcgcaaagac ataccggata gataccgaaa gattgctgat ccggtgctac gaaccggccg
     9481 acgctcccaa gctgcatcag gccatcaccg gcagtctcga acatctccgc ccctgggtgc
     9541 catgggcaag acaggagccc agggaacagg aatggatgga aagctttatc cggatgtgtc
     9601 gcggacagtt cgatctcggt caggacgcca tctacggcat cttcgacggc cggacggaac
     9661 agctcatcgg gggaacggga ctgcataacc gcatcggtaa ggacgcgcgc gagatcggct
     9721 attggatcga tgcccggcat acgcatcacg ggtatgccac tgaagcagta tgcgcgttga
     9781 tcagggtagg cttcgggatc gaacgtctct cccgtatcga gatccggtgc agcccggaaa
     9841 atataccgag ccggaggata ccccaacggc ttggattccg acatgagatg acgctcaaag
     9901 ggaacgccac cgacttgaaa ggacaacccg ccgataccat ggtctggtgc atgacgaaaa
     9961 gcgattatga tctgagcccg ctcagggcga cgacgctgcg cgccatcgat tttatgggaa
    10021 gagagatcgc gctctaatgc gcggcaagct gcttgtaacg caacatcgcc tcgacaaaat
    10081 aataatcggc ataggtgagc gggacgtcga tctcgctgcg acccggcaga ttgccgacgc
    10141 tatgcagaag gatgaatccg ccgttggagc ccactatggc tttataggcg ttggatgaca
    10201 gcgtcacgat gatctgttcg gccacgtcga ggtatccccg accttcggac gccggcacgt
    10261 ccccgccgag ctcgatcaat gccgaagcca gaaccgccgc cgccgaggca tcgcgaagcg
    10321 gcccgggcgt ttgggcagaa gttcctgcgg gtatacccgg cgcattgtaa tcccagtacg
    10381 ggatcttatc tgccggaaga tgggtcagga taaaacgcgc gatatgacgc gcctgttgca
    10441 gataccgcgc gtcatgtgtc caccggtaac acatcgtata cccgtacaaa ccccaggatt
    10501 gtccccgcga ccaggctgaa gagtcggaat aaccctgcgc ggtctttttt gcgatgatgg
    10561 cgcccgtcgc gctgtcataa tcgacgacat ggaacgagct gtagtcggga cgaaaatggt
    10621 ttcgcatggt cgtattggca tgcgtcacgg caatatgata aaaagacgaa tccccgctga
    10681 cggagctggc ccagaacaaa agctcgagat tcatcatatt gtcgatgatg acgggataat
    10741 gccagggttt gctgtcccat gacttgatgc aaccgacggt cgggttgaac cgcgtgctga
    10801 gcgatcgcgc cgcgttgagc aatatctcct tgtagcccgg acggtccgcg acacgcaatg
    10861 cgttgccata tgagcaatac aacatgaaac cgaggtcgtg cgtaccctta ttgtattgtt
    10921 cgggagccaa aagtcgcagc cggtcggtcg cctcgcggag caatgtcgta tcgtgtgaaa
    10981 aggaatacag ctcgaaaagg gtccccggat aaaagccgct ggtccaccaa cccgggtcgc
    11041 tggtctccaa cctgccggta ttggcatacc aggtcttcgg aagacggtcc ggtggaacac
    11101 gtcccatcag tactttgtat tgattgacag cttgccggag agagccgtcg atgagccgta
    11161 ggagtgccgg tttgggttgg agctgatggt gttcctgcgc cgtgaccgcg acggtcagga
    11221 ggcagatcag ggaagagacg agaatgcgca taccgcaaaa tacctatttt tgcgcgtatg
    11281 gaggaaaaac tgaagatcct gggaggcggg ttggaaggtg agctgtactt tgacaagacg
    11341 atgcggaccc tgtatgccac ggatgcttcc gcctatcggg agatgccgct ggcagtcgct
    11401 ataccgaaga ccaccgatga catcaaacgg ctgatcgcat tcgcgaagac gaatcatacc
    11461 tccctgatcc cccgtacggc gggcacctcg ctggcgggac aggtggtggg gaaaggcatc
    11521 gtggtcgacg tatcgcacta tttcacccgg atactcgagc tcaatgcgga tgaacgctgg
    11581 gtgcgtgtac agccgggcgt tatccgcgac gatctcaatc tattccttcg gccgcacggg
    11641 ctgcttttcg gtccggagac ctctacggcc aacagggcga tgatcggcgg tatggtcggc
    11701 aacaattcct gcggcagcaa ttccatcgtt tatcgcagca cgagagaaca tttattggaa
    11761 gtgaaggcta tcctcagcga cggtaccgaa gctacgttta ctgcactcgg gccggacgat
    11821 tttcatgcga aatgcgaggg caacgggctg gaggcggaaa tttaccgatc ggtccggcag
    11881 atgctgagca actacgataa ccaggtcgcc atccgcgaga acttccccaa gaaaagtatc
    11941 gaacgccgga ataccggcta cccgctagat ctgttggtag agacggcgcc gtttaccgcc
    12001 ggcggccccg atttcaattt ctgctcatta ctcgcaggat cggagggcac attggccttt
    12061 ctgaccgaga tcaagctcga cctgtctccg ctgccgccga aagagacggg actgctttgt
    12121 gtccatttcc ataacatcga cgaatcgctc cgggccaatc ttatcgcttt gcgctatcat
    12181 cccactgcca gcgagctcat cgaccacttc atcctggaat gcacaaagga caatatcgaa
    12241 caaagcaaaa accggttctt cgtacagggt gatcccggcg ccattcttgt catcgaattc
    12301 aaaaaggata cgcgggagga gatcgccgcg atcgccgccg cggcggaggc cgatatgcgc
    12361 gcggccggtc tgggctatca ttttccgctg ctattcgggg cggacaccgg caggatctgg
    12421 tcgctacgaa aggctggtct cggattgctg gccaatctgc cgggagacga taaagcggta
    12481 cccgtgatcg aggataccgc ggtggacgta caggaccttc cggagtatat ccgggatttc
    12541 aacgagatcc tgaagaagta tggtctttac tccgttcact acgcacatgc ggggagcggc
    12601 gagctgcatc tccgcccgat catcaacctg aagacggcag aagggaaccg tctgttccgc
    12661 gtcatcgcgg acgagatagc caccctcgta aagaaatacc ggggttcgct cagcggggaa
    12721 catggagacg gccgtctgag gggcgagttc ataccccgga tggtcggcga gcgcaactat
    12781 agtcttttca aatctatcaa aaacacctgg gaccccgccg gcatcttcaa ccccggcaag
    12841 atcgtggaca cgccgcccat gaatgagcat ctgcggtatg aaccgggaca gcaaacgccc
    12901 cccttcccta ccgtcttccg cttccaccag caggacatcc tccagcacgc cgaacaatgc
    12961 aatggcagcg gcgattgccg caagacccat ttgtccggag gcacgatgtg tccatccttc
    13021 atggctaccc gaaatgaaaa ggataccacc cgggcccggg ccaatatcct gcgcgaattc
    13081 ctgacgcgcg acgactcccc gaacaggtat gaccataagg aaatttatga ggtgatggat
    13141 ttgtgtctaa gctgtaaggg ttgcaaatcg gagtgtccgt cgaatgtcga cgttgccaaa
    13201 ctgaaagcgg aattcctgca acattattat gacgctaatg gcgtaccgct gcggacccgg
    13261 ctgatcgctg gttttacgcg gagcgcccag ctgggttcga tggcgccggg agtgtataat
    13321 ttgtttatgc ggagccggtt gctgaaaagg ctgatcggtt ttgcgccgga tcgtagcatg
    13381 ccgtcgctgc ataagaccac gctgcgcaaa tggtggaagg gtcaccggcg cgagccggcg
    13441 tcccgccggg tgtatctttt ctgcgatgaa ttcaccaatt acaatgatgt ggagatcggc
    13501 atcaaggcca ttctgctgct cgagcggctt ggctatgaag tgatcattcc cgatcatatc
    13561 gaaagcggac ggacatggct ctcgaaaggg ctggtcagaa aggctaaggc tatagcgggc
    13621 gagaacatcc gcctgttgtc g
//
LOCUS       DASYSV010000098        10400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2068985, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000098 DASYSV010000000
VERSION     DASYSV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10400
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068985"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VGQ51_03025"
     CDS             complement(<1..491)
                     /locus_tag="VGQ51_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEV9035561.1"
                     /translation="MQDSLKQLFQKEFTKMVAVISRLFGLEHLGIAEDIVSETFLQAA
                     ESWGVKGLPPNPTAWLYLVAKQKALYHFRRNKIFQKKIVPELKGRQKIETDIEEMDFS
                     HEHIRDSQLQMLFAVCDPAIASEAQIGLALRILCGFGIDEIAGAFLTNKETINKRLFR
                     ARE"
     gene            complement(503..847)
                     /locus_tag="VGQ51_03030"
     CDS             complement(503..847)
                     /locus_tag="VGQ51_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV9035562.1"
                     /translation="MKEFALLFRFPEMDVSKFSPQEFQDIAKKWQEWAGGIASQGKMA
                     SNGIRLDMEGKVLRPGGVVTDGPFVEIRERLGSFIVVKAENLEEATTLAHGCPALDQG
                     GSVEIRPIMNYH"
     gene            1039..1380
                     /locus_tag="VGQ51_03035"
     CDS             1039..1380
                     /locus_tag="VGQ51_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV9035563.1"
                     /translation="MLIFRHEDGTKIASPEQMQIWMKQTMDWIGGIAAQNKFVTGTGL
                     PMDDSRVVWHNNVVTNGPFGEIKETIGGYIVVRADSIEEAVEFAKGSPVLQGDGNSVE
                     VRKVAKRDGVH"
     gene            1380..2615
                     /locus_tag="VGQ51_03040"
     CDS             1380..2615
                     /locus_tag="VGQ51_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEV9035564.1"
                     /translation="MEQQQLIPHLFRTEYRKIVSVLGKRFGFDHLETAEDIASETFLT
                     AAQTWPLNGLPENPTAWLYRVAANKAKNYLVRDQLFKGKVSKAIRPPEASSDAAPDQD
                     IDLTPQNINDSQLQMMFAICHPSISTEAQIGLSLRILCGFGIDEIADAFLTSRETINK
                     RLFRARERLREEKIPIEMPAQPEIGRRLDTVLATIYLLFNEGYYSISGDTTLRKDLCL
                     EAMRLCHMLTDNSSTDRPEVNALLALMCFHASRFEARVDSDGETILYDDQDTTLWNDE
                     LIRRGAFYLHRSTASGQLARYHLEATIAFWSTQKTDSPEKWENILQLYNHLLQRHYSP
                     IAALNRTYALARARDKQAAIAEAEKLRLTGNHFYFVLLGELYTGIDNARAREHFENAL
                     ALARSPSDKRIIEKKIGRL"
     gene            complement(2623..3420)
                     /locus_tag="VGQ51_03045"
     CDS             complement(2623..3420)
                     /locus_tag="VGQ51_03045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV9035565.1"
                     /translation="MIRIAFLLLLLPALGCRSQSPEVWDHRQCAVVLTYDDAIDADLD
                     RVIPALDSLGLKGTFYIIGSSSVVAKRLPEWRLAAAHGHELGNHALFHPCDGSKPGRG
                     FVQADFDLSKYTVRRAVDEIRMNNVLLNAIDGKTVRTFAYPCGDLKIGDTLFYKRLEH
                     DFAGARGVTPGMHTIDKVDLDNIDCYGMNGDSGEHMIELVKSAMQTHTLLVFLFHGVG
                     GGHNINVDAAAHSQLLHFLKDHEKEIWIAPMVDVAEHIRAYQQRAGH"
     gene            3530..4753
                     /locus_tag="VGQ51_03050"
     CDS             3530..4753
                     /locus_tag="VGQ51_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9035566.1"
                     /translation="MRKPLPIFFLFAFALQKSTGQSYKQTATLYQVSTSTTIAVTLGA
                     TVLAGDLIVVHVDWDGQTRDIASVTDNKGNIYKKINGPTNWNGANYRAELWYTYGTLA
                     GGTKITAKLNGAPTSFMQIYATEYTGIASSIDPLDQNSAAAGNTAAVSSGSKTTTYTN
                     ELIYGASIGASGSLTTGATFTNRSTANQNIIEDKNVVATGTYNTTFTSGGGNWVAQMA
                     TFISTMSVLPVTLEFFEGRCLNNNVVLDWATTNEKNFAGFTVEGSIDGSNWDDIALIR
                     ATGNSTVTQQYSYTVDPHHSFAWYRLKQIDLDGYFYYSKVIGAGSCGVAKLGVTLFPN
                     PSAGNNLLGIMGGSAGESFQVEILDVTGKIIHRALVGSGPFQLNFGTTLPAGVYYARF
                     VSARSSSVTAFIVKH"
     gene            4825..5499
                     /locus_tag="VGQ51_03055"
     CDS             4825..5499
                     /locus_tag="VGQ51_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035567.1"
                     /translation="MKKICIGALLLCCTGAAFSQRQVPIVLNVYGGYTFPDHVPLGYY
                     NNYNSYGSINGSGQWGAGLEFFVDKLRALELSYQYMGTHMPFYNWEGQTNSGNDKCTI
                     QYIMLGGNNYVPTHGNLMPYAGVGLGVGLMNYTYYNGYSENLTKFAWNIHLGVKVKTE
                     SAVSIKLQAYLQSIAQGVGVGVGFGTGGAGAGVTTYSSMLQFGLGGAICFALGGKGSG
                     GHHSDQ"
     gene            5631..6959
                     /locus_tag="VGQ51_03060"
     CDS             5631..6959
                     /locus_tag="VGQ51_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV9035568.1"
                     /translation="MKRPLFSVLFLLTLTVCSGQPSADRPAWVRRANIYEVNVRQYTP
                     AGTFKAFAKHLDRLKAMGVEILWFMPINPISHTDRKGSLGSYYAVSDYTAVNPEFGTL
                     ADFRMLVASAHAKGMKVILDWVPNHTGADHRWLRQHPDFYKKDSSGKAAVPFDWTDTR
                     QLDYDNPVLRDSMIAAMQYWVRQTDIDGFRCDVAWNVPGDFWKDCITRLRKLKSLFFL
                     AEGDKPYLARNGFDALYPWDFFHKMISVAAGERPAFALDSILHQYDTAYPKGTLEMFF
                     TSNHDENSWNGADYKTFPGVAHAPFAVLSQTLPWDIPLIYSGQEEPVLRALSFFEKDS
                     IGWQHFGRATFYKKLLSLRKRNPALDADASFTKLKTGNDQAVYAFLREKTGKRVLVLL
                     NFSPTSQQVSIRRPDLIGGTPTEIFTGKTTSPGMKVPVLPPWGYMVFEYP"
     gene            complement(6956..7843)
                     /locus_tag="VGQ51_03065"
     CDS             complement(6956..7843)
                     /locus_tag="VGQ51_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEV9035569.1"
                     /translation="MEVQDLSKVHGLAGRLFEWPKDRKGWEEYRLTKEQLQQYEELGY
                     VSGIKMLDESQIAVLKEELAALADPKHPGHSLFYEFSTNESTDPAKILFHALGAWRIT
                     PGFHDVLWNPRFLMAASQLLGNRAVRFWHDQLFCKPAKHGGVVAWHQDYSYWTRSVPM
                     QHLTCWCGLDAADEENGCLYYVPGSHHWGLLPKPILAGNMDDLVGVLTEEQRAAFHPV
                     AIPLPAGYATFHHPLMVHGSYANTTGRQRRAFVINVFADGTRSNADEQLLAGTPMVPK
                     GDKLAGDFFPLLFEPMSAH"
     gene            8002..9546
                     /locus_tag="VGQ51_03070"
     CDS             8002..9546
                     /locus_tag="VGQ51_03070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV9035570.1"
                     /translation="MPTTAFTRSLLFSLTLGLTIIRPAIAQDLPRGIPEKEGVPSTAI
                     SDFLNAASRGRTEFHSFMLLRHGKVLAEGWWNPYGPHLKHTLYSVSKTFTMTAVGLAI
                     KEKKLKLTDKVVSFFPNDLPAAVSPNLAELTVKDALMMADGQDPEPRAVSTDTNWARA
                     FLAVPIVNKPGTKFLYNSMGTYMLSAIVQRATGQKVLDYLKPRLFDPLGIIDEDWETS
                     PQGVNTGGWGLRLSTEDMAKFGQMMLQKGKFNGRQIVPRDWVEEATTMKILQDPNASP
                     AARDSSDWLQGYCFQMWRCRHNGVRADGALGQFIILLPDQDAVVVMTAETPSMQEEIN
                     LVWQYLLPSMQNWAYKEDPDGDARLKKQLSALALPPLEAKASAADFSVNGRTYALQAN
                     DWNLTSIGFSFQGGLWKVTIHDSKGSWPFSFGDGKWEHATTDRPGPTITSRAVNSAVG
                     LAPFQVAGSYHWVDENTLELVLRYIESPHTEEMVCHFDGDNIKIEDHYSFDYGKAPVV
                     LTGSAQ"
     gene            complement(9589..10059)
                     /locus_tag="VGQ51_03075"
     CDS             complement(9589..10059)
                     /locus_tag="VGQ51_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035571.1"
                     /translation="MKKLLLAATVFAAAALSNTTQAQIHFSINIGDQPSWGPTGYEHV
                     EYYYIPDIQCYYYVPSHEFVYLDGGGHWTFSRELPPAYPRFDLYHSRKIVMNEPQPYL
                     HFDEHRRQYEHFRGDPHGQKFIRDSHEDRYRYHYHDQHEADEEWRHRGGGHDHH"
     gene            complement(10172..>10400)
                     /locus_tag="VGQ51_03080"
     CDS             complement(10172..>10400)
                     /locus_tag="VGQ51_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MutS2/Smr-associated SH3 domain-containing
                     protein"
                     /protein_id="HEV9035572.1"
                     /translation="QVNEKVRRKFDAKYTEIGGDAQVGNKVMMKKNHQVGTVREIRGK
                     KAVVQVGLVPITVDISDLIVVQDRPQEESAG"
BASE COUNT         2423 a         3001 c         2652 g         2324 t
ORIGIN      
        1 ttctccctgg cccggaaaag ccgtttgttg atcgtctcct tattggttaa gaatgccccg
       61 gcgatctcat cgatgccgaa tccgcaaagg atacgcaggg ccaacccgat ctgcgcctcg
      121 ctggcaatag cgggatcgca gacggcaaac agcatctgca gctgactgtc ccggatatgc
      181 tcatgcgaga aatccatttc ttcgatatcg gtttcgatct tctgccggcc tttcagctcg
      241 ggaacgatct tcttttgaaa gatcttgttc ctcctgaaat ggtacagcgc tttttgtttg
      301 gctaccaggt agagccaggc ggtcggattt ggcggaagac cctttacccc ccagctttcc
      361 gccgcctgca ggaaggtctc gctcacgatg tcctccgcta tcccgagatg ctccagcccg
      421 aagagccggc ttatcaccgc caccattttg gtgaactctt tttgaaacag ctgttttaaa
      481 gaatcctgca tggcgatcgc ccttaatggt aattcatgat cggcctgatc tctacgcttc
      541 cgccctggtc cagcgcgggg cagccatgcg ccagcgtcgt agcctcttcg agattttccg
      601 ccttcaccac gataaagcta cccagtcttt ccctgatctc tacgaacggt ccatcggtga
      661 ccacgcctcc gggccggagc acttttcctt ccatgtccag tcgaatgcca ttgctggcca
      721 ttttgccctg cgaagcgata cccccggccc attcctgcca ttttttggcg atgtcctgga
      781 attcctgcgg cgaaaacttg ctgacgtcca tttcgggaaa ccggaacaat aatgcaaact
      841 ctttcataaa tcttttttta attgtttaca gattttttta tccgaataag taccggacct
      901 gcaaaaattg gacatggcgg caaaaaaaat tattttacaa aagattctcc aggatgtcca
      961 atccctgccc cgtcgcttgt actaggtatg tcaatcgaat cgtcaatact attaaaattt
     1021 acgaaaatgg atgaatttat gttgattttc aggcacgaag acggcaccaa aatagcctcg
     1081 ccggaacaaa tgcagatctg gatgaaacaa actatggact ggatcggagg tatagcggca
     1141 caaaacaagt tcgtcaccgg caccggtctg ccgatggacg acagccgcgt agtctggcat
     1201 aacaatgtgg tgaccaacgg cccgttcggt gagatcaagg agaccatcgg aggctatatc
     1261 gtcgtcaggg cggacagtat cgaggaagcg gtggaatttg ccaagggcag ccccgtactc
     1321 cagggcgacg gcaatagcgt ggaagtgcgg aaggtcgcca aaagggacgg agtacattaa
     1381 tggaacagca acaactgatc ccgcatctct tccggacgga atatcgcaag atcgtctccg
     1441 tcctgggcaa acgcttcggc ttcgatcatc tcgagacggc cgaagacatc gccagcgaga
     1501 cctttttgac ggctgcacaa acctggccgc tgaatggcct gccggaaaat cccacagcct
     1561 ggctctatcg cgtcgccgcc aataaggcca aaaactatct ggtgcgggat cagcttttca
     1621 aaggaaaggt ctcgaaggcc atccggcccc ccgaggcttc ctccgatgcg gcccctgacc
     1681 aggacatcga tctcacgccg cagaacataa atgacagcca gctgcaaatg atgttcgcga
     1741 tctgtcatcc ctccatttcg acggaagccc agatcggcct gtcgctccgc atcctgtgcg
     1801 gttttggcat cgacgaaata gccgacgctt ttcttaccag ccgggagacc atcaacaagc
     1861 gtctgttccg cgcccgcgaa aggctgcgcg aagaaaagat acctatcgag atgcccgccc
     1921 agcccgagat cgggcgtcgt ctcgacaccg tgctcgctac catctatctc ctgttcaatg
     1981 aaggatatta ttccatcagc ggcgacacta cccttcgcaa agacctgtgt ctggaagcca
     2041 tgcggctttg ccatatgctg accgacaatt cttccaccga ccgtccggag gtgaatgcct
     2101 tgctggcatt gatgtgtttt cacgcctccc gtttcgaggc ccgcgtcgac agcgacggcg
     2161 agaccattct gtatgacgac caggacacca ccctctggaa cgatgagctc atccgcaggg
     2221 gcgcattcta cctgcaccgc agcacggcat ccggccaact cgcccgctac cacctcgagg
     2281 ccacgatcgc attctggagt acccaaaaaa ccgattcacc ggaaaaatgg gagaacatcc
     2341 tgcagctcta taatcatctg ttacagagac attattctcc catcgccgcg ctcaatcgca
     2401 cgtacgcgct cgcccgcgcg cgagacaagc aagccgccat cgccgaagcc gaaaagcttc
     2461 ggctgaccgg taatcatttt tattttgtcc tgctgggcga gctctatacg ggtatcgata
     2521 acgcgagagc acgggagcat ttcgaaaatg cactggcact cgcccggtcc ccgtcggaca
     2581 aacgcatcat cgaaaagaag atcgggcgac tttaatgaat cgtcagtgcc cggctcgctg
     2641 ctgataagcc cgtatatgtt cggcgacatc gaccatgggg gctatccata tttctttttc
     2701 atggtccttc aggaaatgta aaagctgact gtgcgcagcg gcgtctacgt tgatgttatg
     2761 cccgccgcct accccatgaa atagaaagac cagcagggta tgcgtctgca tggcggactt
     2821 aacgagctcg atcatatgct ctccgctgtc cccgttcatt ccataacaat cgatattgtc
     2881 cagatcgacc ttatcgatcg tatgcatccc gggagtaacg ccacgcgcac ccgcgaaatc
     2941 gtgttccagc cgtttgtaga acagcgtgtc gccgatcttc agatcaccgc agggataggc
     3001 gaaggtgcgt acggtctttc cgtcgatggc attcaacagg acgttgttca tccggatctc
     3061 gtcgacggcc ctccgcacgg tatacttgct cagatcgaaa tccgcctgaa cgaagccgcg
     3121 tccgggttta ctcccgtcac atggatgaaa gagcgcgtgg ttccccaact catgtccatg
     3181 cgcagcggca agccgccact ctggcagtcg tttagccacc accgatgatg aaccgatgat
     3241 atagaaagtt cccttgagcc ccagagagtc cagcgcggga atgacacgat cgagatcggc
     3301 atcgatggca tcgtcatagg tcaatacgac cgcacattgc cggtgatccc atacttcggg
     3361 cgattgcgac cggcatccca gcgcaggcaa taataaaagg agaaaggcta ttctgatcat
     3421 ggctcaaaat aacaaaaaag gacagctttt cggtaataat ctgacaatgc ttccgttata
     3481 ccccgcggta tacccatcga accgtagtta gctaaagacc gatatccaaa tgcgcaaacc
     3541 cctgcccata ttctttctat ttgcattcgc cctgcagaaa agcacggggc aaagctataa
     3601 gcagactgct actctttacc aggtatccac ctccacgacc atcgcggtca ccttaggagc
     3661 caccgtcctt gcaggcgatc tgatcgtcgt tcacgtcgac tgggatggcc agacccgcga
     3721 tatcgcctcg gtcaccgata acaaaggcaa tatctataag aaaataaacg gtcccaccaa
     3781 ctggaacggc gcgaattacc gggccgagct ttggtatacc tacggtaccc tcgccggagg
     3841 caccaagatc accgccaagc tgaatggagc acccaccagc tttatgcaga tatatgctac
     3901 cgaatatacc ggcatcgcct cgagcatcga cccgctggat caaaactccg cagccgcggg
     3961 aaacacggca gcagtcagca gcgggtctaa gacgaccacg tataccaacg agctgatcta
     4021 tggcgccagc atcggcgcct ctggttcgct gacgacagga gcgactttca ccaaccgatc
     4081 caccgccaat cagaatatca tcgaagacaa gaatgtcgtc gccacgggca cctataacac
     4141 gacctttacc agcggcggag gaaactgggt agcgcaaatg gctaccttca tttcgacgat
     4201 gtccgtgctc cccgtgacgc tggaattctt tgagggaaga tgcttgaata acaacgtggt
     4261 gctcgactgg gccacgacca atgagaagaa ttttgccggg tttacggtag aaggcagcat
     4321 cgacggcagc aattgggacg atatcgctct gatcagggcg acaggcaatt ccaccgtcac
     4381 tcagcaatat tcgtataccg tcgatcccca tcattcgttt gcgtggtacc ggctgaaaca
     4441 aatcgatctc gacggctatt tttattattc gaaggtgatc ggagcaggca gctgcggggt
     4501 cgcgaaattg ggcgtaacgc tatttcccaa tccttccgcg ggcaataatc ttttgggtat
     4561 catgggcggt tccgcgggtg agtccttcca ggtggagatc ctcgacgtca ccggtaaaat
     4621 aatccaccgg gcattggtgg gctcaggtcc cttccaattg aatttcggca cgacgcttcc
     4681 agcaggcgtc tattatgcac gattcgtttc ggcacggtcc tcctccgtga cggccttcat
     4741 cgtaaaacat tgatcttctt tttcccgacc cgaatggcgg aaccatctgt ctgaatgtgt
     4801 cctatacctg tcaatcgggt aattatgaaa aaaatatgca tcggggcact cctgctgtgc
     4861 tgtacgggtg ccgctttctc ccagcggcag gtgccaatcg ttctgaatgt gtatggcggc
     4921 tatacctttc ccgaccatgt accactgggg tattacaaca attacaacag ctatggatct
     4981 atcaacggct ccggccaatg gggcgccggt ctggaattct tcgtggacaa gcttcgggcg
     5041 ctggagctca gctaccagta catggggacg cacatgccat tctataattg ggaggggcaa
     5101 accaattcgg gcaatgacaa gtgtacgatc caatacatca tgctcggtgg gaataattat
     5161 gtcccaacgc acggcaatct catgccttat gccggcgtcg gtctcggagt aggcctgatg
     5221 aattacacct actacaacgg ctactccgaa aacctcacga aattcgcctg gaacatccat
     5281 ctgggtgtga aggtcaaaac ggaatccgcc gtttccatca aattacaagc ctatctgcaa
     5341 tccatcgcgc aaggtgtggg cgtcggcgtg ggcttcggta ccggcggcgc cggggcgggt
     5401 gtgaccacct attcctcgat gcttcagttc ggactgggcg gcgctatatg tttcgcgctg
     5461 gggggaaagg gatccggagg acatcattcc gaccaatgac cttcgatgcc cgatcgatca
     5521 cctccggctg ccgatcgatg acgtctaccc atcgatgccc gttgccgatc gataccttcg
     5581 acgaagaagc ctgaattgac tatcttcggc aaaacttctt tttgctgctc atgaaacgcc
     5641 ccctcttttc cgtcctcttc ttgttaacgc tgaccgtctg ttccggtcag ccctccgccg
     5701 atcgcccggc ctgggtccgt cgcgcgaaca tctatgaagt gaatgtccgt caatataccc
     5761 cggcgggcac cttcaaagcc ttcgcaaaac atctcgatcg tcttaaagcc atgggtgtgg
     5821 agatcctgtg gttcatgccc atcaatccca tcagtcacac cgatcgcaag ggcagccttg
     5881 gcagctatta tgccgtctcc gactatacgg ccgtcaatcc cgagttcgga accctggccg
     5941 atttcagaat gctggtagca tccgcgcatg ccaaaggaat gaaggtgatc ctcgattggg
     6001 tgcccaatca caccggcgcc gatcaccgat ggctcagaca acacccggat ttttacaaaa
     6061 aggatagctc tggaaaagct gccgtccctt tcgactggac ggatacccgt cagctcgact
     6121 atgataaccc ggtattgcgc gacagcatga tcgcagccat gcaatactgg gttcggcaaa
     6181 cggatataga cgggttccgt tgcgatgtcg cctggaatgt tcccggcgac ttttggaagg
     6241 attgtatcac ccggctcaga aagctgaagt cgctgttctt tcttgccgag ggcgacaagc
     6301 cctatctggc aaggaacggt ttcgatgcgc tgtatccctg ggattttttc cataagatga
     6361 tcagcgtggc ggcgggcgag cgccccgctt tcgcgctcga cagcatattg caccaatacg
     6421 acaccgccta tccgaaaggg acgctcgaaa tgttcttcac gagcaatcac gatgagaaca
     6481 gctggaacgg cgccgattat aagaccttcc ccggtgtcgc gcatgctccc ttcgctgtcc
     6541 tgagccaaac gctcccatgg gatatcccgc tgatctatag cgggcaggaa gaacctgtac
     6601 tgcgggccct gtccttcttc gaaaaagatt ctatcgggtg gcagcatttc ggcagagcga
     6661 ccttttacaa aaagctattg tcccttcgca aacgaaatcc cgcgctcgac gccgatgcat
     6721 cgttcacaaa actcaagacc ggcaacgacc aggccgttta tgcctttctg cgtgaaaaaa
     6781 ctggcaaaag ggtactcgtc ttgctgaact tttctccgac atcgcagcag gtatcgatca
     6841 ggcgtcccga tttaattggt ggcacaccaa cggaaatctt tacgggtaag acaacttcac
     6901 ccggaatgaa ggtgcccgtg cttcctccat ggggatatat ggtattcgaa tatccctaat
     6961 gcgccgacat aggctcgaac aacagcggga aaaaatcacc tgcgagctta tcccctttcg
     7021 ggaccatcgg cgtacccgcc agcagctgtt catccgcatt cgatcgggtc ccgtcggcaa
     7081 atacattgat gacgaaggcc cggcgttgcc gccctgtagt attggcatag gacccgtgaa
     7141 ccatcagggg atgatgaaag gtggcataac ccgcgggtaa gggtatcgcc accgggtgga
     7201 atgccgcgcg ttgttcttcg gtcaacactc cgacgagatc gtccatattt cccgcgagga
     7261 tcggtttcgg caacagcccc caatgatggc ttccgggaac ataatacaaa caaccatttt
     7321 cctcatccgc cgcatcgagt ccgcaccagc acgtgagatg ctgcatcggc acgcttcggg
     7381 tccaatagga ataatcctgg tgccaggcaa ctaccccgcc atgtttggca ggtttgcaaa
     7441 acagctgatc atgccagaac cgcaccgccc tgtttccgag caactggctg gcagccatca
     7501 gaaatcgcgg attccaaagt acgtcgtgaa atcctggtgt tatacgccac gcgcccagcg
     7561 catggaaaag gatctttgcc ggatcagtgg attcgttggt ggagaattca taaaaaagcg
     7621 aatgaccggg atgtttcggg tccgccaatg ccgccagctc ctctttcaac accgcgatct
     7681 gcgactcatc gagcattttg attcccgaaa catagcccaa ttcctcgtat tgctgcagct
     7741 gttctttcgt cagccggtat tcctcccagc ctttccggtc cttcggccac tcgaagagtc
     7801 gccccgccaa accatgtact ttactaagat cttgtacctc cataacgcat atattttaag
     7861 attccattca atactttttc cgatgcgtcg atcgcatttg aaccaaacat actgaataaa
     7921 gcgatcccac ccaagccccc acagacatgg cccggcaatt caatttttgg tttatcttca
     7981 ctcctacata aacccatcgc tatgccgacc accgctttca cccgcagcct cctgttttcc
     8041 ctcacgcttg gcctgacgat tataaggccg gccatcgccc aggatcttcc caggggcatc
     8101 cccgagaagg aaggcgtacc ctccaccgcc atcagcgatt ttctcaacgc cgcctcgcgt
     8161 ggccgtacgg aattccacag cttcatgctg ctgcgccacg gcaaggtcct ggcggaaggc
     8221 tggtggaacc cctatgggcc tcacctgaaa catacgcttt attcggtgag caagaccttc
     8281 acgatgacgg ctgtcggtct ggccatcaaa gaaaaaaagc tgaagctcac cgataaggtg
     8341 gtctccttct ttcccaatga tctcccggca gccgtaagcc ccaatcttgc ggaactcacc
     8401 gtaaaagacg cgctgatgat ggccgacgga caggatcccg aaccgcgtgc agtttctacg
     8461 gataccaact gggcgagggc ctttcttgcg gtgcccatcg tcaacaaacc gggtacgaaa
     8521 ttcctgtaca actcgatggg aacctatatg ctctcggcca tcgttcaacg cgccacgggc
     8581 caaaaggtgc tcgattatct caaaccacgc cttttcgatc cgctgggtat catcgatgag
     8641 gactgggaga cgagcccgca gggtgtcaat acgggaggat ggggattgcg tctgagtacg
     8701 gaggatatgg ccaaattcgg acagatgatg ctgcaaaaag ggaaattcaa cggccgtcag
     8761 atcgtcccgc gcgattgggt ggaggaagca actacgatga agatcctgca ggaccccaat
     8821 gcctcacccg cggcaaggga ttccagcgac tggctgcagg gttattgttt ccagatgtgg
     8881 cgctgtcgtc acaacggtgt aagagccgat ggcgcgctcg gacagttcat catcctcctg
     8941 ccggaccagg atgcggtggt ggtaatgaca gccgaaacgc cgagtatgca ggaagagatc
     9001 aacctcgtat ggcagtacct gctgccttcg atgcagaact gggcctataa ggaggatccc
     9061 gatggcgatg cgcggctcaa aaagcagctg tccgccctcg ccttgccgcc actcgaagca
     9121 aaggccagcg cggcggactt ctccgtcaac ggcaggacct atgcgctgca ggccaacgac
     9181 tggaacctga cctccatcgg tttctcattt caaggtggtc tatggaaggt gacgatacac
     9241 gattcaaaag gctcctggcc tttttctttc ggagatggca aatgggagca cgcgacgacg
     9301 gatcggcccg ggcccaccat tacgtcacgc gcagtgaaca gcgcggttgg gctggcgccc
     9361 ttccaggtcg ccgggtctta tcattgggta gacgaaaata cgctcgaact cgtgttacgc
     9421 tatatcgaaa gtccgcacac cgaggagatg gtttgtcatt tcgacggaga taatatcaag
     9481 atcgaagatc attatagctt cgactatggg aaggcgccgg tcgtgctgac cgggtccgcg
     9541 caataagtga ggcccccggc ttcgccgggg gcctcctcag atagtctttt agtgatggtc
     9601 gtggccacct ccccggtgcc tccactcttc gtcggcctca tgctgatcat gataatggta
     9661 tctgtacctg tcttcatgac tatcacggat gaacttctgg ccatgcggat caccgcgaaa
     9721 atgttcgtat tgcctccggt gctcgtcaaa atgcagatag ggctgcggct cgttcattac
     9781 gatcttccgg ctatggtaca ggtcgaaacg aggataggcc gggggaagct cgcgggaaaa
     9841 ggtccagtgt ccgccgccat ccaggtaaac gaattcatga gaagggacat aatagtagca
     9901 ctggatatcg ggaatataat aatattccac gtgctcatag cccgtcggac cccaggaagg
     9961 ctgatcgccg atgttgatgg aaaaatggat ctgcgcctgc gtggtgtttg acaaggccgc
    10021 cgccgcgaag accgtcgcgg ctaaaaggag tttcttcata ttggaggttt ttaagttact
    10081 accgtcaacc ggcaaagtca gtgccaaaag atgtgagaga accgttaacc ggcggcattc
    10141 ggcgtggcgt ggcggggcgg caagcgaact cttaaccggc gctttcttcc tgcggacggt
    10201 cctgaacgac aatcagatcg gagatatcta ccgttatcgg aacgagcccc acctgcacga
    10261 cggccttttt cccgcggatc tcgcgaacgg tgcccacctg gtggttcttc ttcatcatca
    10321 ccttattgcc tacctgcgca tcccctccga tctcggtata tttggcgtcg aacttccggc
    10381 ggactttttc attcacctgt
//
LOCUS       DASYSV010000099         7509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2196002, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000099 DASYSV010000000
VERSION     DASYSV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196002"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VGQ51_03085"
     CDS             complement(<1..544)
                     /locus_tag="VGQ51_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV9035573.1"
                     /translation="MRRLLFSVMMILAAIMTSAQGDTSWFYDSHFHLTNYIQEGITPQ
                     QMLGIMGGKVGRSTLFGIPLQQTWAYGNSGDFAPNYYLASDAPLYYYSFTDAYIAMAW
                     RSLTREQQLRFDPMITGFNPADMYAADHIRRVLTVFPGVFEGIGEFTIHKEFVSSKVA
                     GEKASLINPALDRILDFAGEA"
     gene            complement(560..1891)
                     /locus_tag="VGQ51_03090"
     CDS             complement(560..1891)
                     /locus_tag="VGQ51_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035574.1"
                     /translation="MDHSNGIRSISLFILLLTVYRVSFAQVDQTTSTRDSTGYTRSER
                     ETFPNTAAGELTPGRGFNLVKTEKGSLNVSIYAIVRYLNQLPGNQTWYDHLGRQQNFV
                     GRNDFYWHRTMIWFTGFVLTPRLTYTATVWTIMTTQQTLVYGNLQYAVNKYINLGMGI
                     MPNISVRSMQGPFPFYSSTDRTMGEESLRAGFTNGFFVRGQVVPRLTYILMLGDNLST
                     LGVAAANLTRTLSKGAGLVWMPSTGEFGQRGGIMDFEHHDKVATRFGVNYTYDRDSRF
                     NDMSNPSPDNVQVRMTDGVLFFQTGALADGVTVQDATYQMLAVDAGLKYKGFQVQLEG
                     YDRWLTDFNADGPVPVSSLHDIGYSVQLNQMVVPKTLGIYAIHSYFFDAFNRHPWEVG
                     GGFNFYPKQSRSWRINAQVHHVYKSSAGGTFGLYNAGQTGTTITIGSDILL"
     gene            complement(1932..2969)
                     /gene="glsA"
                     /locus_tag="VGQ51_03095"
     CDS             complement(1932..2969)
                     /gene="glsA"
                     /locus_tag="VGQ51_03095"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEV9035575.1"
                     /translation="MKYASLLSRKLAVLMLPLLGPLMVLKTNAQGVALSKQAIQSALD
                     EAYNQFKDLREGKNADYIKELAQVDPNIFGIALVTTDGTVYTKGDIGSMVSIQSVSKV
                     FTMAKNIDLLGPKAIQDKIGVDATGLPFNSIVAVEEHRGKEINPLVNPGAIASTSLMP
                     GDDSAAKWASVVQVESDFAGRPLGLNWPVYVSEAGDNLRNQAIAHLLFAYGRMYFDPV
                     QATDIYTKQCALNVSAKDLAEMAATLANGGINPVTKKRCVGPQTTAYTLAVMATAGLY
                     DDSGIWLYNSGLPAKSGVGGGLLAVCPGKFGIAVVSPPLDQAGNSVKAQRVIQYVVEK
                     LKVNPYLVQPR"
     gene            complement(2997..4223)
                     /locus_tag="VGQ51_03100"
     CDS             complement(2997..4223)
                     /locus_tag="VGQ51_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HEV9035576.1"
                     /translation="MCLKLRATLALLFLFSLSRSFAQDQSTDTSLIKTFEIYGFAMAD
                     FGYNTKQINPAWFDALRVTRLPTYKNQYAPDGTFYASIRQTRFGVRGWTPTPIGNLKA
                     VFEFDMFGVGGDEGQTTMRPRHMYGELGRFLLGQTNTPFMDGDVFPNTVEYWGPTGIV
                     FFRNIQLRFAAMQGDNELFFALERPGASADAGTEAGRIELDSIVPKLELPDVSAHYKK
                     SGSWGHIQIAGMLRDIKWKDIHESGGYDLSGSVLGWGLHLSTVLNLGKMNVFRGSIVH
                     GEGVENYMNDAPFDVGVEVDSTDPQKPLKGKALPVTGWSAYLEHNWSPVWQTVIGYSA
                     THIENTTQGSTTAYKDGQYGTITMVATPFTNFMAAVEAEYGKRANFGNDFTSDALKIQ
                     VSFKYTFSQSFYSRRK"
     gene            4395..5513
                     /locus_tag="VGQ51_03105"
     CDS             4395..5513
                     /locus_tag="VGQ51_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035577.1"
                     /translation="MLCKTAFAICFLCGSLSIFGQYTTDKVVGQKHEALIDSLKKTEY
                     PYLLPIWGQKVISKGYNLPYSAGLSVQYLWQQSDILINNLQVGFNNGPKYQLDEIIRF
                     DGAQATSNGVNIRPDFWLFPFLNVYAIFAQSRTSTAVNCGLWVPDSAGFHKILDFNTK
                     ANFNATTVGFGVTPTFGIGGYFVVLDFNFSWSDVDALDKPAFVFNFGPRLGKNFSFRK
                     AEQSLAVWVGGFRVKINSGTSGSLSTSQLFPTDQWQSKLDTGYMKVAASQENVDAWWA
                     NLTPAGQKNPVNVAKYNTANAALMRAGEMLDAASTAVTNAGNSTVQYSLEKKQKHMWN
                     FIIGSQFQINKSWMIRAEYGFLGSRMQFIGGLQYRFGL"
     gene            5713..6261
                     /locus_tag="VGQ51_03110"
     CDS             5713..6261
                     /locus_tag="VGQ51_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035578.1"
                     /translation="MRTLLFIAACTSLGLSAHSQDEAVIIKASKQISKQMTPQQVIDS
                     LNAHFPNAHAVKYYKAPADAVARGWSVSKDDNFSHGDQIDYYTISFKNEDLQYYGLFE
                     ADGRLIRSKQENTVEHVPDAVKTSVQNLGASHPGWKVVSKKYFKNLDSNSMEEYYEII
                     ATDGNKVKSLYYKPDGTLMEIK"
     gene            complement(6311..6898)
                     /locus_tag="VGQ51_03115"
     CDS             complement(6311..6898)
                     /locus_tag="VGQ51_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035579.1"
                     /translation="MKTAIIVSLMAIGPVCGFAQGNFMISYPISFPMGNMHDYIGNTS
                     FRGISLEFNKKVAPNQTVGIETGWNVFYQHVDQKVYTEGSSSISGVQYRYTNSVPIIA
                     GGKYYFEGHGHIHPYAGVGLGTLYVSRTTDFGIFRIESDAWQFCIRPELGIDAKIGPA
                     ESLFVGAKYYWAFNGGDLDAQPYVSINIGFKFTNL"
     gene            complement(6909..>7509)
                     /locus_tag="VGQ51_03120"
     CDS             complement(6909..>7509)
                     /locus_tag="VGQ51_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEV9035580.1"
                     /translation="PKGAQYYSDFDLVYTNHSDSVDFSRRKTYARPDSIIKITGDVFN
                     GSGGTDEPTFLPSAYTEVIVKQVDENMAAYGWTKVDKNANPDVVLLMSASNTSYMYWY
                     YDWGYWGWYYPGYYPGWGWYYPGYYPPYVSTYTTGTLLIQMTDQQQVVNPTSGTTANV
                     PVIWVCVLNGLVDGSTADVAARVQTNLNQAFVQSPYLKH"
BASE COUNT         1754 a         2035 c         1930 g         1790 t
ORIGIN      
        1 cggcctcgcc ggcgaaatcc aggatacggt ccagcgccgg attgataaga ctcgcctttt
       61 cccccgccac tttcgaggag acgaattcct tgtgaatggt gaactcgccg ataccttcaa
      121 aaacaccggg aaatacggtt aacacccgac ggatatgatc ggccgcatac atatcggcag
      181 gattgaagcc ggtgatcatg gggtcgaacc gcaattgctg ttcgcgcgtc agggatcgcc
      241 aggccattgc gatataggca tcggtaaatg aataatagta caaaggcgca tcgctcgcga
      301 ggtaataatt gggagcgaaa tccccgctgt ttccataggc ccaggtttgc tgcaaaggga
      361 ttccgaataa ggttgacctg cctacctttc cgcccatgat cccgagcatt tgctggggtg
      421 tgatcccctc ctggatataa ttggtcagat ggaaatgcga gtcatagaac caggaagtat
      481 cgccctgcgc agacgtcatt atcgctgcaa ggatcatcat aacggaaaat aacaatcgcc
      541 tcatagcttt cagatttgat caaagtagta tatcgcttcc tatcgtgatc gtcgtgccgg
      601 tctgacccgc attgtataac ccgaaggttc cgccggccga ggatttatag acatgatgca
      661 cctgcgcatt gatcctccag ctcctgcttt gtttgggata gaaattgaac cccccgccca
      721 cctcccaggg gtgccggttg aaggcatcga aaaaatagga atgtatcgca tagatgccga
      781 gcgttttcgg aaccaccatc tggttaagct gtacgctata gcctatgtcg tgcagcgagc
      841 ttaccggcac cggtccatcc gcattgaaat ccgtaagcca ccggtcgtat ccttccagct
      901 gcacttgaaa ccctttatat ttcagaccgg catcgacggc gagcatctga taggtagcat
      961 cctgcacggt aaccccatcc gcgagcgcac cggtctggaa gaaaagaacc ccgtcggtca
     1021 tcctgacctg cacattatcg ggcgacgggt tactcatatc gttgaaccgg ctgtccctgt
     1081 catacgtata gttgacgcca aaacgcgtgg cgactttgtc gtggtgttcg aaatccatga
     1141 ttcccccacg ctgtccgaat tccccggtgc tgggcatcca gaccagtccg gctcctttcg
     1201 acaatgtccg cgtgagattg gccgcggcta ctcccagtgt gctgaggttg tcgcccagca
     1261 tcaggatata ggtgagacga ggaaccacct ggccacggac aaagaacccg ttggtaaacc
     1321 ctgcccgtag gctctcttcg cccatggtcc ggtcggtaga tgaataaaag gggaagggcc
     1381 cctgcatcga ccggaccgaa atattcggca tgatccccat gccgagattg atgtatttat
     1441 tcacggcata ctggaggttg ccatagacga gggtctgctg cgtcgtcatg atggtccata
     1501 cggtggccgt atacgttagc ctgggcgtca gcacgaatcc ggtgaaccag atcatggtcc
     1561 tgtgccagta aaagtcgttc ctgccgacaa agttctgttg tcttcccagg tggtcatacc
     1621 atgtctggtt gccgggcagc tggttcagat agcggacgat agcatagatg ctgacgttga
     1681 gactaccctt ttcagtcttc accaggttga accctcgccc cggcgtcagc tctccggcag
     1741 cggtattggg gaaggtttcc ctttcgctcc tggtataacc cgtcgaatcg cgcgtgctcg
     1801 tggtttgatc cacctgcgcg aaggaaaccc ggtacaccgt caataacaaa ataaaaaggc
     1861 ttatcgaacg tattccgttg ctatgatcca tcgggtcaac tatttaattt aatttcagag
     1921 ataaaccctg atcatctggg ttgaacgaga taaggattga ctttgagttt ttcgactaca
     1981 tactggatca ctctttgggc cttgacgcta ttacctgcct gatcgagggg cggcgatacg
     2041 acggcgatcc cgaatttgcc cggacagacg gccaataagc cgccgccaac accgctcttt
     2101 gccggaagcc cggaattata taaccagatg ccgctgtcat catataaccc cgcagtggcc
     2161 attactgcca gggtataagc tgtggtctgc gggccgacac agcgtttttt cgtcaccggg
     2221 ttgatgccgc cattggccag cgtggccgcc atctcggcca ggtcttttgc cgagacattg
     2281 agggcgcatt gtttggtata gatgtctgtc gcctgcacgg gatcgaaata catcctgcca
     2341 taggcaaata gcaaatgcgc gatggcctgg ttcctcagat tgtcgcccgc ctcgctgaca
     2401 taaacgggcc agttgagccc cagcggacgg ccggcaaaat cgctctccac ctgtacgacg
     2461 cttgcccatt tggccgcgga atcatccccc ggcatcaggc tggtggatgc gatagcgccc
     2521 gggttgacga gggggttgat ctctttaccc ctgtgctctt cgacggcaac gatggaattg
     2581 aacggcagac ccgtcgcgtc gacgccgatc ttatcctgta tcgccttagg tcccaacaga
     2641 tcgatattct tcgccatggt gaatactttg gagacgctct ggatagacac catggaaccg
     2701 atgtcgcctt tcgtatagac ggtgccgtca gtggtgacca gcgcgatgcc gaagatgttc
     2761 ggatccacct gcgcaagctc tttgatataa tccgcatttt tcccttctct gagatccttg
     2821 aactggttgt aagcctcatc caacgccgac tggatggctt gtttgctcaa tgcaactccc
     2881 tgagcgttgg tcttgaggac cattagcgga cccagcaggg gcagcatcag gactgccagc
     2941 ttcctcgata aaagagatgc gtatttcata cttcaaattt tttgattcgg gagaatttat
     3001 ttcctgcgtg aataaaagga ttgtgagaac gtgtacttga acgaaacctg gattttgagc
     3061 gcatccgacg taaaatcatt tccgaaattg gcacgcttgc catattccgc ttccactgct
     3121 gccatgaaat tcgtgaaggg ggtggccacc atcgtgatgg tcccatactg tccgtcctta
     3181 taggcggtgg tagacccctg cgtcgtattt tcgatatgcg tagctgaata accgatgacc
     3241 gtctgccaaa cgggcgacca gttatgctct agataggcgc tccacccggt gacaggcagc
     3301 gctttgccct tcaatggttt ttgcggatcc gtggaatcca cttcgacgcc tacgtcgaag
     3361 ggtgcatcat tcatatagtt ctccacacct tcgccatgta cgatggagcc cctgaataca
     3421 ttcatctttc cgaggttgag caccgtgctc aaatgcagcc cccacccaag gacgctgccg
     3481 ctgagatcat atcctcccga ttcgtggatg tccttccact tgatgtccct cagcataccg
     3541 gcgatctgta tatgccccca tgagccgctc tttttataat gcgccgatac atcggggagc
     3601 tcgagtttcg gcacgatgct gtccagctcg atccttccgg cttccgtccc tgcatcggcg
     3661 cttgcgccgg ggcgttccag ggcaaagaac aattcgttat cgccctgcat ggccgcgaac
     3721 cgcagctgga tattccggaa gaatacgatg ccggtaggtc cccagtattc aaccgtgttg
     3781 ggaaagacat cgccatccat gaaaggcgtg tttgtctggc cgagcaggaa ccggcccaac
     3841 tctccgtaca tatgccgcgg ccgcatcgtt gtctgtcctt catcccctcc tacaccgaac
     3901 atgtcgaatt cgaacaccgc tttcagattg cctatgggtg tcggcgtcca tccacggaca
     3961 ccaaaacgtg tctggcgtat gctggcataa aaagtcccgt caggggcgta ttggttctta
     4021 taggtgggca gcctggtgac cctcaacgca tcaaaccagg ccggattgat ctgtttggta
     4081 ttataaccga aatccgccat ggcgaatcca tagatctcga aggtctttat gaggctggtg
     4141 tcggtggatt ggtcctgggc gaaggatctt gaaagactaa acagaaaaag caatgcaaga
     4201 gtagcgcgca gttttaggca catatcaaga gcatttaata gttgaggaat ctatttcagc
     4261 cttatgacac cataaaagtt gcgggaccgc caaccccctt gcattatttg gcatatttaa
     4321 cggctaaaaa cggcggcgcc atccgcccgt ccgtgtcgaa tagtaaggat accgtctcat
     4381 aaaatcaggt taccatgctt tgcaaaaccg cttttgccat ctgctttttg tgtggctctc
     4441 tttcgatatt cgggcagtac actaccgata aggtggtcgg ccaaaaacat gaggccctca
     4501 tcgacagtct gaaaaagacg gaatatccct atctcctgcc catctgggga cagaaggtga
     4561 tcagtaaggg gtataacctc ccctattctg ccggtctcag cgtccaatat ctatggcagc
     4621 aatccgacat cctgatcaat aatctccagg tggggtttaa caacggtccg aaatatcagc
     4681 tcgatgagat catccggttt gacggcgcgc aggcgaccag caacggggtc aatatacggc
     4741 ccgacttctg gttgttcccc ttcctgaatg tctatgcgat cttcgcccaa tccaggacca
     4801 gcacggccgt gaattgcggt ctctgggtcc cggacagcgc gggcttccat aagatcctcg
     4861 acttcaatac gaaggcaaat ttcaatgcga ccaccgttgg cttcggagtc acgccaactt
     4921 ttggcatcgg cgggtatttt gtagtactcg atttcaattt ctcgtggagt gatgtcgacg
     4981 cgctcgataa acccgccttc gtcttcaatt tcgggcccag gctgggaaag aatttcagct
     5041 ttcggaaggc ggaacagtcg ctggccgtgt gggtaggagg attccgggtc aagatcaaca
     5101 gcggcaccag cgggtccctg tctacctcgc agctgttccc caccgaccaa tggcagagca
     5161 agctggatac aggatatatg aaagtggccg ccagccagga gaatgtagac gcatggtggg
     5221 caaacctcac tcccgccggg caaaagaacc cggtcaatgt agccaaatac aatacggcca
     5281 atgctgcgct gatgagggca ggtgagatgc tggatgctgc ctccaccgcc gtcaccaatg
     5341 ccggcaattc tactgtccag tattcgctgg aaaagaaaca aaagcacatg tggaatttca
     5401 tcatcggcag ccagttccag atcaacaaaa gctggatgat ccgggccgaa tatggctttc
     5461 tcggatcgag gatgcagttc atcggggggc tgcaataccg gtttgggttg tgatcgccgt
     5521 gcccgccctt tcccttcgcg gcccccgggt catccgatgc cgtctctcgc ctggcggggt
     5581 tcattttccg gcgttcccgg ggtcatccgc cgcctctcac cgggtcctcc ccggccaccc
     5641 tgccgtgcct ctttttcgtc ggaattggcg gaagatacct ttcctgtgtg tcataggcaa
     5701 aaacaaacaa atatgaggac tttactattt atcgccgcgt gtacatccct gggcctctcc
     5761 gctcactccc aggatgaggc cgtaatcatc aaggcgtcca agcagatcag caagcaaatg
     5821 acgccgcaac aggtgatcga ttccctgaat gcgcattttc ccaatgccca tgccgtgaaa
     5881 tattataagg cgcctgcgga tgcagtcgcc agggggtgga gcgtctcgaa ggacgacaat
     5941 ttttcgcacg gcgatcagat cgattactat acgatcagct tcaagaacga agacctccag
     6001 tactatggac ttttcgaggc cgacggaagg ctcattcgca gcaaacagga gaatacggtc
     6061 gagcacgtac ccgatgcagt gaagacctcc gttcaaaatc tcggcgcctc tcatcccgga
     6121 tggaaggtcg tctcgaaaaa atatttcaag aacttagact ccaattccat ggaagaatat
     6181 tatgaaatta tcgcaacgga tggcaataag gtgaagagct tatattacaa gccggacggc
     6241 acgttgatgg agatcaaata agacagaggc cgggcccgaa gggcccggcc tctcacattt
     6301 acacccttct tcacagattc gtaaatttaa accctatgtt gatcgatacg tagggttgcg
     6361 catccaggtc tccgccattg aaggcccaat agtatttagc gccgacaaac aatgattccg
     6421 ccggtccgat cttcgcatcg atgccgagtt cgggacgtat gcaaaattgc caggcatcgc
     6481 tttcaatcct aaagatcccg aagtcggtag tacggctcac atacagggtc cccaatccga
     6541 cacccgcata aggatgtata tgaccatgcc cctcgaaata gtatttgccg ccggcaatga
     6601 tgggtacaga attggtatac cggtattgca caccgctaat ggaagacgag ccctccgtat
     6661 atactttctg atcgacatgc tgataaaaga cattccatcc ggtttcgatg ccgacggtct
     6721 gattgggcgc tacctttttg ttgaattcca ggctgatgcc ccggaaactg gtgttgccga
     6781 tatagtcgtg catgttgccc atcgggaaag agatgggata ggagatcata aagttcccct
     6841 gtgcaaagcc gcaaaccggg ccgattgcca ttagcgagac gataattgct gttttcataa
     6901 atcttttttt aatgttttaa gtaaggggat tgtacaaaag cctgattgag gttggtctgc
     6961 accctggcag ccacgtcggc agtactgccg tctaccaatc cgttgagcac acagacccag
     7021 atcacgggaa cattggcggt cgttccggaa gtgggattga ccacttgctg ttggtcggtc
     7081 atctggatca gcaatgttcc ggtagtatat gtcgatacat aggggggata ataaccggga
     7141 tagtaccaac cccagccggg ataatagccg ggataatacc atccccagta accccaatcg
     7201 taataccagt acatatagga agtattcgac gcactcatca ggaggacgac atccggattg
     7261 gcattcttgt ctaccttcgt ccaaccgtat gctgccatat tctcgtccac ctgtttgacg
     7321 atcacttccg tataggcgga cggtaggaag gtcggctcgt cggttccgcc actgccattg
     7381 aaaacatcgc cggtgatctt gatgatgcta tccggtctgg catacgtctt tcgccgggaa
     7441 aaatcgacgg aatcggaatg gttcgtgtat acgagatcaa aatccgaata gtactgggcg
     7501 cctttcgga
//
LOCUS       DASYSV010000100         7282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_533896, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000100 DASYSV010000000
VERSION     DASYSV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533896"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..2301
                     /locus_tag="VGQ51_03125"
     CDS             97..2301
                     /locus_tag="VGQ51_03125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV9035581.1"
                     /translation="MRNIHYLLPSLLITTALCAQPAQEPVKLTDLLKIHTVGDIHLSK
                     DGRLAVFTLTTIEPDTSAKTSKWDYKYVNQLYLVPSDGSVRPRQLTTAREGAGQPDWS
                     PDGKQIAFVRTADGKPQIFLLSLEGGEPTQLTHFRYGASSPKWSPDGKEILFSARIGL
                     TQLIRDSALNPGHALPAWPDEKPGFTADADLQPSTVKGDPDGNIDQIRAWLAGNVTDH
                     KAKVVNKLNFQDEMEVNPETTFTHWFIQSLQPASGSAEVTPPPQPASGPTEVTHGFFT
                     YNSAEYSPDGKFLYFAAHIDSTQDPDRDLESEIWFADRDGGHMHKVLGEKGKVFGNPR
                     VSPDGKWMAYTVSPISFVSVPQLYVQSLTGNTPAIHIAFDRAPGGLTWSDDDRYLYFI
                     AQSNGGAPVYRVPPASPKVEQLFDYNTGTTAFALENGHLLFARTQPSDPSELFTADAA
                     GQKATIITAFNAEWLSHRRLSLPEKHSFSNSLGQTIEYWVMKPIGYTAGQRYPVLLDI
                     HGGPSAMWGPGEPSMWHEFQYFCGHGYGVVYCNPRGSGGYGLDFLRANVKDWGAGPTS
                     DVLTALDKAVAEGWADTARLVVSGGSYAGYLTAWIVGHDHRFKAACAQRGVYDLATFF
                     GEGNAWRLVPNYFGGYPWEPATKAILQHESPITYVANITTPLIIFHGESDRRTGFVGG
                     EMLYRSLKVLGRPVEYVRHPGATHELTRSGDNRQRMDQMLRTYEFFERYIKH"
     gene            2442..3011
                     /locus_tag="VGQ51_03130"
     CDS             2442..3011
                     /locus_tag="VGQ51_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9035582.1"
                     /translation="MIEAISAGKDLNDAIRFLYRQHSEAISSFIIHYGGNEQDADDIF
                     QETVVAFIDIVRKGKYRMEATVRTFLASIARNCWFNELKKRGRSDHRDRQFELGRDKD
                     EADVSHHITELEQKRQLRKLVDQLGEACRKVLLLFYYENMSMKEIVSHLPYENEQVVR
                     NKKYKCLQQLTGLIKSNPAIARQMQETLK"
     gene            3020..3841
                     /locus_tag="VGQ51_03135"
     CDS             3020..3841
                     /locus_tag="VGQ51_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035583.1"
                     /translation="MTPEQTHILIDRLDESLSGNPATGPSLEQNPEASQEWQYLHMAV
                     DAIRDAGLYEQVSAVRAALKAEQEGTRVVPFAETSGSASGATVETSEPTGAVVRSIYR
                     RYSLRAAALILVITSSAFLYKYLSISSQSLYDRYYSSFELNTARGAGDEQPIVKAYDA
                     KDWTTVLSLAGTAKSRDNQTDFLAGMADLELKRYDDAITHFEEIIAVNTHAGTDFYQD
                     EAEYYLAISWLARGNVNEAMPILEKIRADAHHQYHEKVAKMSFFDLRLAQYKENK"
     gene            complement(4170..6989)
                     /locus_tag="VGQ51_03140"
     CDS             complement(4170..6989)
                     /locus_tag="VGQ51_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEV9035584.1"
                     /translation="MYPRIAVSLSTFLLLAGLVFGYPGGMPGGLLALYRRSDSLFHLT
                     RSTPSTDSMALAGFSTVIKGLEALGSPPDGGTDSLLAGALLRKGVLLDAAGDFEGAKT
                     AYCKALSHHRIDDSLVFVSEVYAGATYYNLNHFDSANYFLLRAMTKVGRFNDRDDEVR
                     LYNTLGVLYYDNGNYRQGKDYFDRALEMVRGRRPLDTAAAVSLQTNIATSFFRLEQYQ
                     QALSIFRQLLSYRPLTNYVNMNMGRAYAALSEYPAALACFRKVDAGKIPGVWNEMANA
                     QQQLHRPDSCSWYLHRLQTAAHADPTRFNVLDLGVNALYEAGWLRAQGKRADALVSLQ
                     RAIVIFSRNFKNTDVNSNPSNFTGSFAEYRLFDALVQKAELFRQLYGGEEGEGHRSAR
                     PKTDETYLKASYAAYTSALSLLRYIEKSYATDEAKLFLKKTSGPAHAGALSVALQLYK
                     LHPKEDYLEQAFLISERSKASVITTQLEEREFMGMTGTAQVLLHNLNDCKYNIARLDV
                     RIEGTSDSAELVSLTREREAGELELSRLQQQMEQNGEYYKLKYEDTSPGIHDLQGELT
                     GGQAMISLYAAGGVLHVFVVTKDDFKYTHIDSLSRVQADVASWLDALKTTGNGRRFHG
                     EAIGRRLYTELVNPIQAVAGGQREWILIPDGFLYLLPWESLPADADGRQYLLETTTIS
                     YRWSSKLLREGGEGPEKAMGVLSFAPFEKEGDGSFSRLAASGEEIAGLAGAQYLDRNA
                     TKAEFLQTVNRYPIVHLATHAVSSTDNAAASFIAFYPVRHSPMEDRLFLEELYGLDLR
                     PTRLVIISACETGEGEVVAQEGVISLARAFAYAGCGSTINSLWKADDRATSFILRQFY
                     VHLKEGETKARALQLAKLDYLKSDAVDKSPAYWAHLVLTGDSSPLYSGSLSLWWWLLL
                     VPVGVVVWGIGRKRKKKSTIQRVRA"
     gene            complement(7082..>7282)
                     /locus_tag="VGQ51_03145"
     CDS             complement(7082..>7282)
                     /locus_tag="VGQ51_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035585.1"
                     /translation="YYKLNDGSNQALQNISVQIQYFSSKSSISSGVLGGILNSVNSLL
                     SGNVISTSANPRPPLIIITRRE"
BASE COUNT         1758 a         2177 c         1824 g         1523 t
ORIGIN      
        1 gccaggaaaa gaaatagcaa atacgcgaat gaacgaagtg gcattaggtc ttttaaggat
       61 gaatatacgt aaatttatgg ccatcaaaac ccgcgcatga gaaacatcca ttacctgctg
      121 ccgtcgttat tgatcacaac cgcgctgtgc gcacaacctg ctcaggaacc cgtcaaactc
      181 accgatcttc tgaaaataca cacggtcgga gatatccatc tcagcaagga cggtcggctt
      241 gccgtcttca cgctcaccac catcgaaccc gacacttccg caaaaacttc caaatgggat
      301 tataaatatg tcaaccagtt gtacctggtc ccttccgacg gaagcgtccg gccacgtcag
      361 ctcaccacgg ccagggaagg cgccggccag cctgactgga gtcccgacgg caaacaaatc
      421 gccttcgtcc gtaccgccga cggcaagccg cagatattcc tcctttccct cgaaggaggc
      481 gaaccgacac agctgacaca tttccgctat ggcgcctcct cacccaaatg gtcacccgat
      541 ggaaaggaga tcctcttctc cgcccgtatc ggtctcactc agctgatcag ggatagtgcg
      601 ctcaatcccg gtcatgccct ccccgcctgg cccgatgaaa aacccggttt taccgccgat
      661 gccgatctgc agccgtctac cgtcaaaggc gatcccgacg gcaatatcga ccagatcagg
      721 gcctggctcg ctggcaatgt cactgaccac aaagcgaaag tggttaacaa gctcaatttc
      781 caggacgaaa tggaggtcaa ccccgagacc acttttacgc actggttcat ccaatccctc
      841 caacccgcat caggctccgc agaggtcacg ccaccgccgc aacccgcatc cggccccacc
      901 gaagtcacgc acggattctt cacatataac agtgccgaat attcgcccga cggcaaattc
      961 ctctattttg ccgcgcatat cgacagtaca caggaccccg accgcgacct tgaatccgag
     1021 atatggttcg cagatcggga cggcggacat atgcataagg tcctcggcga aaaaggaaag
     1081 gtcttcggca atccccgcgt cagtcccgac gggaaatgga tggcctacac ggtcagcccg
     1141 atctcattcg tatcggtgcc ccagctgtat gtccagtcgc tgacgggcaa tacccctgcg
     1201 atccacatcg ccttcgaccg ggctcccggc ggtctcacct ggagcgacga cgaccgttac
     1261 ctctatttca tcgcacaatc caacggcggc gctccggtct atcgggtacc accggcctct
     1321 ccgaaggtcg agcagctctt cgactacaac accggcacca ccgcctttgc gctggagaat
     1381 ggacatctcc tgttcgcgcg aacccaaccc tcggatcctt ccgaactctt caccgccgac
     1441 gccgccggtc aaaaggcgac gatcatcacc gctttcaatg cagaatggct cagccaccgc
     1501 cggcttagtc tgcccgaaaa acatagcttt tcaaattcac tcggccagac catcgaatac
     1561 tgggtgatga aacccatcgg ctataccgca ggacagcgct atccggtcct gctggatata
     1621 catggcggtc cttccgccat gtgggggccc ggtgaaccct ccatgtggca tgaattccag
     1681 tatttctgcg gacatggtta cggcgtcgtt tactgcaatc cgcgtggctc gggaggctat
     1741 ggtctcgact tcctccgcgc caatgtcaag gactggggcg ccggtccgac ctcagatgta
     1801 ctgacggcgc tggataaggc cgtagcggaa ggctgggccg atacggcaag actcgtcgta
     1861 tccggggggt cttatgcggg ttatctgacg gcctggatcg tgggtcacga ccatcgcttt
     1921 aaagctgcct gcgcgcaacg cggcgtttat gacctcgcca cctttttcgg cgaaggcaat
     1981 gcctggcggc tcgtacccaa ctatttcggc ggctatccat gggaaccggc cacaaaagcc
     2041 attttgcagc acgaatcgcc cattacctat gtggctaata tcaccacccc tcttattatc
     2101 ttccatggcg agagcgaccg gcggacaggc ttcgtcgggg gtgaaatgct gtaccggagc
     2161 ctgaaggtcc tgggacggcc cgtggaatac gtccggcacc ccggggcgac ccatgaatta
     2221 acccgcagcg gcgacaaccg gcagcgcatg gatcagatgc tccgaaccta cgagtttttc
     2281 gaacgttaca taaaacattg attgcaaata ttttacattt ggcataccat tggctctcca
     2341 tttctcgtta atagaaaaaa tgtttcactt tcaacggtta caaatcacca cctggaattg
     2401 actaattaat gaaagccgag gaaaaatata ctgatgctga attgattgaa gctatcagtg
     2461 ccggtaaaga cctgaatgat gccattcggt tcctctaccg gcaacactcc gaagccatta
     2521 gctcttttat aatccattat ggcggcaatg aacaggacgc cgatgacatc ttccaggaga
     2581 cggtcgttgc ctttatcgac atcgtaagaa aagggaaata ccgtatggaa gccaccgtca
     2641 ggacttttct tgcctccatc gcgcggaact gctggttcaa cgaactgaag aaaagaggtc
     2701 gttcggacca tcgcgaccgg caattcgagc tgggacgcga caaggatgaa gccgatgtaa
     2761 gccatcatat cacggagctc gaacaaaaac gtcagctccg gaaattggtg gaccagctcg
     2821 gggaagcgtg ccggaaagtg ctgttgctgt tctattatga gaacatgtcc atgaaagaga
     2881 tcgtcagtca cctgccttat gagaatgagc aggtggtacg aaataaaaaa tacaagtgcc
     2941 ttcagcaatt gacaggtttg ataaaatcta atcctgccat tgcaaggcag atgcaggaaa
     3001 ccctaaaata gtcaatgcca tgacacccga acagacacac atattgatcg accgtcttga
     3061 tgaaagtctt tccggaaacc cggctaccgg cccgtcgctg gagcagaacc cggaagcttc
     3121 gcaggaatgg caatatctgc atatggccgt ggatgccatc cgtgatgccg gtttgtacga
     3181 gcaggtctct gccgtcaggg ctgccttgaa agcagagcag gaaggtacgc gtgttgtccc
     3241 gtttgcggag acctccgggt cggcaagtgg cgcaaccgtg gagacctccg aacctaccgg
     3301 tgctgtcgtt cggtccatct atcggagata cagcctccgg gctgccgccc ttatcctggt
     3361 catcaccagc agcgcattcc tgtataaata tctttcgatc agcagccagt cgctatacga
     3421 ccgctattat tcctccttcg agctgaatac cgcccgcggc gccggcgacg aacagcccat
     3481 cgtaaaggcc tacgacgcca aagactggac gaccgtattg tcactggccg gcacggctaa
     3541 aagccgggat aaccagaccg atttcctggc aggcatggcg gatctcgagc tgaaacgata
     3601 cgacgacgcc atcacccatt tcgaagagat catcgccgtc aatacccatg cgggcaccga
     3661 tttctaccag gatgaggccg aatactatct cgcaatcagc tggctggctc gcgggaatgt
     3721 caacgaagcg atgcctatcc tcgaaaagat acgtgccgac gcccatcacc agtatcatga
     3781 aaaagtggca aaaatgtcct ttttcgacct cagactggcc caatacaagg aaaataaata
     3841 aaaaaccggg gttgacccgg ttacgtcccg atctccggcg attactaatt agtgatcgta
     3901 cccattaact ctttaggaag aacccccgat agcctgaaaa acccgaccgt agttcgtatc
     3961 ctttggaaaa gccctatcca acccatgaaa accgccaatc cttgaaaaac cgccgcatcc
     4021 ttgaaaagcc acccaatcct cgaaaaccac ccacaagatc cgccctgcga aaaccgaagc
     4081 cccgtttgca aaacccaatc ctctggaacc actgaaaccg aaaaaagtcg gcgtaataat
     4141 taccctttaa gaaactctat caccggccat caagcacgaa ctctctgaat cgtcgacttt
     4201 tttttccgtt ttctgccgat cccccacacc actaccccca ccggcaccag cagtaaccac
     4261 caccacaaag aaagagatcc cgaatacaaa ggactactat cccccgtcaa tacgagatgc
     4321 gcccaatagg caggactctt atcgacggca tcgcttttca gataatccag cttggccagc
     4381 tgcaacgccc tggcctttgt ctccccctcc ttcagatgta cataaaattg ccggagaata
     4441 aaggacgtcg cccggtcatc ggccttccaa aggctattga tcgtgctgcc gcaacctgca
     4501 tacgcgaaag ccctcgcaag gcttatcacc ccttcctggg ccactacctc cccctctcct
     4561 gtctcgcaag cgctgataat gaccagccgg gtgggacgca gatcgagacc atacaattcc
     4621 tcgaggaaca aacgatcttc catcggcgaa tgccggacag gataaaaggc gataaaggaa
     4681 gccgctgcat tgtcggtgga ggatacggcg tgggtggcga gatggacgat aggataacga
     4741 ttcacggttt gcaggaactc cgcctttgtc gcattacggt ccaaatattg agcccccgcc
     4801 agtcccgcga tctcctcgcc cgaagcggcc agccggctaa aactcccgtc cccctccttc
     4861 tcaaacggcg cgaaggacaa cactcccatc gccttttccg gtccctcccc gccctccctg
     4921 agtaattttg acgaccaccg gtagctgatc gtcgtcgtct ccaaaagata ctgccggcca
     4981 tccgcatccg ccggcaggga ttcccagggc aacagataca ggaacccatc ggggatcagg
     5041 atccattccc gctggccccc cgcgacggcc tgtatcggat tgaccagctc ggtatacaat
     5101 cgtcgtccga tcgcctcacc atgaaaacgc cggccgttgc ccgtcgtctt gagtgcatcc
     5161 agccagctcg cgacatcggc ctgcacccgc gaaagactat cgatatgggt atatttgaaa
     5221 tcgtccttcg tcacgacgaa aacatgcaat acccctcccg cggcataaag actgatcatc
     5281 gcctggccgc ctgtcagctc accctgaaga tcgtggatac ccgggctggt gtcttcgtat
     5341 ttcagcttgt aatattcgcc gttctgctcc atctgctgtt gcagacgcga aagctccagc
     5401 tcacccgctt cgcgttcccg ggtcagcgag acaagctccg cactatccga ggtgccttca
     5461 atgcgtacgt ccagccgggc gatattgtat ttgcaatcgt ttaaattgtg aagcagcacc
     5521 tgcgccgtgc ccgtcatccc catgaactcc cgctcctcca gctgggtggt gatcaccgag
     5581 gccttgctcc gctcgctgat gagaaaggcc tgctcgagat aatcttcttt gggatgcagt
     5641 ttgtacagtt gtaaagcgac ggataacgca ccggcatgcg ccggaccgct cgttttcttg
     5701 aggaaaagtt tggcttcgtc ggtagcatag ctcttctcga tatagcgcaa cagcgacaac
     5761 gcagaggtat aggcggcata ggaagccttc agatatgtct cgtcggtctt cggcctcgcg
     5821 ctccggtgcc cctcgccctc ttcgcccccg tacaattggc ggaacagttc ggctttttgg
     5881 acgagcgcgt cgaatagtcg gtactcggca aaggagcccg taaaattcga aggattggaa
     5941 ttgacatcgg tattcttgaa attgcgcgaa aaaataacaa tggcccgttg gagactaacc
     6001 agggcatcgg cgcgctttcc ctgagcgcgc agccacccgg cctcataaag ggcgttgact
     6061 cccagatcga gcacgttgaa acgcgtagga tccgcatggg cagccgtctg cagccgatgc
     6121 agataccagc tgcaggaatc cggccggtgc agttgctgct gcgcattggc catctcgttc
     6181 cagactccgg gtattttccc ggcgtcgact ttgcggaaac aggcgagtgc ggcgggatat
     6241 tcgctcaggg cggcataggc cctgcccata ttcatgttga cataattggt cagcggacga
     6301 taggacaaca gctggcgaaa gatggacaac gcctgctgat actgttccag ccggaaaaag
     6361 gaggtggcaa tattggtttg aagactcacc gctgcggcgg tgtccagcgg ccttcgtccc
     6421 ctaaccatct cgagggccct gtcgaaataa tcttttccct ggcgatagtt gccattgtcg
     6481 tagtaaagga cccccagcgt gttgtaaagc cgcacttcat catcccggtc gttgaaccgg
     6541 cctaccttgg tcatcgcacg taacaggaaa taattggcgc tgtcgaaatg gttcaggttg
     6601 taataggtgg cgccggcata gacctcgctg acgaagacca gcgaatcgtc gatacgatga
     6661 tgcgacagcg ctttgcaata ggccgtcttc gccccttcaa agtcacccgc ggcatccagc
     6721 aggacaccct ttcgtaaaag cgcgcccgca agcagactgt cggtcccgcc gtcgggcggg
     6781 ctccccaatg cctccaaacc cttgataacg gtgctgaaac ccgcgagcgc catgctgtcg
     6841 gtggaaggag tgctccgggt aagatggaaa agactgtcgg atctccggta tagcgcgagc
     6901 agcccccccg gcatgcctcc gggatagcca aagacaagcc ccgccaatag caaaaaggtc
     6961 gataacgata cggcgatcct cgggtacatg gtgcgtactt tggataaatc tccaaaaaaa
     7021 aggcgaaaga tggaaatcct tcgccttgcc gtatccgata tttgtgattg ctaaagactt
     7081 tttactctcg tcgcgtgatg atgatcaggg gtggtcgggg gttcgcggaa gtggagatga
     7141 cattaccgct gagcaggctg ttcaccgaat tgagtatccc gccgagcacg cccgaactga
     7201 tggaagactt actgctgaag tattggatct gcacgctgat attctgaagc gcctggttac
     7261 ttccgtcatt cagtttataa ta
//
LOCUS       DASYSV010000101        10536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2452662, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000101 DASYSV010000000
VERSION     DASYSV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452662"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3736
                     /locus_tag="VGQ51_03150"
     CDS             <1..3736
                     /locus_tag="VGQ51_03150"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEV9035586.1"
                     /translation="AGMLKTPSHTLVGLDLPNSHVQVKDILVFAPQLASQPVFRNPND
                     VWQVNGKIRGTFDVLDVQQLQFSGIRDLRLDMAGVIRHPFDSRRIQGDINLRNLSGSR
                     TALISLVPRGTLPSSIRIPARYDLHGRLAGSMDAITTDLTLHTSSGTVIVKGFARNFR
                     STTAASYDIAVRTRALDLGTILQDSAQWGAVTSDFTLRGTGLDIHSAKSRFSGRITAA
                     TFRHYHYHDLTIDGSVADQQATLHSAIDNTAVSFELQASADLSRKFPALRLDWQIDTL
                     DLHAVGLTTDTMQFKGHLAAQFEDTNPDSLQGSLRVTKMDLLQGHQHLITDSLILLAT
                     RESDREHIQLHSEMADLDLDGHYLLTQVPTALEHTISRYYHLEGFRDTAFAPQDWTLR
                     MTLRTSPLVLAMMPSLRGTDTVGGVIAYNSDRDDLQLGIRTPRIDLGTQFIHNVAITA
                     GTGNGQLQYAVTISDAHGSGLVLYKTSVAGALKDDKLTTTLLLKDVKGKDRYRLAGQL
                     EKLSGGLKLHFDPDSLMLNYDRWQVSRDNFIQYDSSGIVARDFTISNNGDSLSLNSRG
                     TTGAAPLDVSFGHFRLSTISRLANQDSVIADGILNGNAEVRNVTTNPLFTSDLKIENV
                     SYKADTLGDLTLKVNNKKANAFSADIALQGKNNDIAISGDYYTGESKMDLKLDLRRIN
                     LAAFTGAAQGVMDKMKGYLAGQFTIAGTFDKPNVRGNLYFDSTVVTPTISGETLDVSK
                     DRIAFDEDGFNFSEFTLKDSAGNKLILDGNVFTKDYHSFAFDISLNAQNFRLINAPEN
                     SSRQFYGKLNLDAAINLEGKMEAPKIDGTIRVNKNTNFYYGLPPDNPEVGDRVGIVRF
                     VDHRTGDTLVDKKALALNAKKTEIKGLDVSLNLLTDTAALLNIVIDPRSGDELNVRGR
                     SNLVFQMEKSGKMDLTGSYQVNGGYYTVSFEVLKRKFNINPNSIITWTGDPTKATVDL
                     TASYTAMTPSIDLVSNVIGDLPTTEQNKFRQKLPFLVTLKMEGDMLKPTITFDVSLPT
                     TTLTLWPDVDQRLTELRSQPSEMNKQVFALLLLGRFVGEDPLNSQAGGGSTIGNLAFS
                     SASQILTNQMDQIAASLIKEVDIHFDLNNQQDWTTGNEIDYTELDVTVSKTLMHDRLR
                     VGVGSSFDVVGTGAPQQAPSNLAGTVDASYKLSKDGRYLLRVYRQNQYEAVVLGQVIE
                     TGVGFVFTVNYDKLREIFHRAKGDQIQPTKTTTPKTTSPK"
     gene            3733..6042
                     /locus_tag="VGQ51_03155"
     CDS             3733..6042
                     /locus_tag="VGQ51_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEV9035587.1"
                     /translation="MTGIRRMLRLFLSMGLFWSALAACNESKYLAGNETLYSANKTKV
                     ESSVAMSNKEKKRLADDDMEDYLRPKLNGKILGLRVKLWVYNIAGTTHKTKGFKHWLK
                     YKIGEPPVLASPAILANNAQVLQSHLENKGFFHDTVIVTTFVKDKHLTATYTAQIGPR
                     YTIGKLDYPDDSDDVSNRIDSLKKRSLLKTGDFFDLETIKEERVRIDDRLKNHGFYYF
                     NPDDLIVDLDTAVGDHKVDMFMRLKDDMPDPARKVYRIDSITAYVNPAGKADTNRSHA
                     YITPEGYRIVDSAHYLRPHVFSRTLLIKPKDIYKENDHNSSLSRLVSLGVFQFVKARF
                     EPATSADTNKMLNVYYYLTPAQSKQLRFEVTALTRSDNTTGTEVALDWRHRNIFHGGE
                     LFSIRVYGGLEGQSVSTTEKSLTRRTGVDATLTIPKIVAPFKLNTSGKFVPKTKIAAG
                     YDIFENNSLYTLTSSRVSFGYQFKNRVTSENGITVLGITYVRPSNITDSFQAAIDKNH
                     NLYYAIEPQLIIGPSWNFNYSSVFDPRHARRPNNYYFNANVDFSNNLLGLISGANFNR
                     TGHRKEILGVPWAQYMRFEADFRHYLNFSKYNMLVSRFDAGIGIPYGNSASLPFVKAF
                     FAGGTNDIRAFRARALGPGHYYAGDPNVNAFIPDQPGDIRLEFNLEYRSNLWGLVRWA
                     VFTDWGNVWTLRNDPNRPGAVFTSAFLKDFAVGAGAGLRFDLTVMVLRIDVAVPLRTP
                     WKPEGSKWNFHSATDVSDMVLNLAIGYPF"
     gene            6170..6517
                     /locus_tag="VGQ51_03160"
     CDS             6170..6517
                     /locus_tag="VGQ51_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035588.1"
                     /translation="MKDEKSKQQRQQPQENDMGQKKSSDQQNQGKSSSDHQSSGKQKP
                     GQQPTGQHPTGEDPSRKSQQQKGGMGHPMDVKEGDRRGDIENPDQQDEPGKKVNIDDD
                     PDQTKKKVPNMHK"
     gene            6571..7065
                     /locus_tag="VGQ51_03165"
     CDS             6571..7065
                     /locus_tag="VGQ51_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEV9035589.1"
                     /translation="MKSKLYEHEVKGPARMTGRNKKILSILDDLIRINIDRITGYEKA
                     AHEENTFDPEWRARFYRLATDSRSYVNNLHAEVIRLGGPPVTQATISGKIHLHWLEGK
                     NRFEGKDFLSRLAACRAAEMSVQTAYHQALDEPEHLPPAIYDLLETQLWALERAYQAF
                     ITLV"
     gene            7394..7918
                     /locus_tag="VGQ51_03170"
     CDS             7394..7918
                     /locus_tag="VGQ51_03170"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV9035590.1"
                     /translation="MLSQPSLLFLREKILDLKNAIFISQHTSLLKMPTTIVSIEKMDE
                     LGQLWFFVPRPQQALHEFDREFPVRLEFFRKGRDFFLHASGKAFIVTDPEDINGWLED
                     DIREKADDRLVLIRVNLLKAEYFQTPAPHTQTRWWRDLRNQLHVWIFNTRPGYKPYHL
                     ADAPMPMLSYRPVA"
     gene            8076..8669
                     /locus_tag="VGQ51_03175"
     CDS             8076..8669
                     /locus_tag="VGQ51_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEV9035591.1"
                     /translation="MKQLSIYLLSGALTLACFSCGNNQPSSTEAAKDSNATKMSDSNS
                     TNAMASTVSKADQSFAVDAADGGMTEVKAGQMAEEKGMAKDVKEYGNMMVKDHTELAD
                     KLKSIATTKNITLPGNISPDKQKDLDDLDKKTGKDFDKAFLDMMVSDHKKVISAFQDE
                     AKNGSDADIRAFADSSLHILQHHLDEAQKCQKMEKKM"
     gene            8800..9762
                     /locus_tag="VGQ51_03180"
     CDS             8800..9762
                     /locus_tag="VGQ51_03180"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV9035592.1"
                     /translation="MSPQNFTLSLQDGLFFRTVDTVGVCFERILDHSPSRVWEVLTQP
                     AHLSRWLAPAEIKGGAGGEIRLQMTGGTMGGRILQWKENALLEYIWYNGTVVRWELLQ
                     EGKDRTRLVFTHTHVQQIQLLDAAKGWHYHLDLLSIELDGKEIPSNPVERWDTITREA
                     TVRYKEQLGGVRQTPPPFVLERVFDATADRVWQALTNKDEIRQWSFDIADFEPEEGFD
                     FTFDGEKDGERFVHLCRVTEVVPGKKLAYSWRYENVRGVSHVTWELFPEGRKTRLRVT
                     HEGLEKLAHAGEPWARHNFEAGWTAIFDQGLRSWLQQAPMALAR"
     gene            complement(9817..>10536)
                     /locus_tag="VGQ51_03185"
     CDS             complement(9817..>10536)
                     /locus_tag="VGQ51_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEV9035593.1"
                     /translation="LCWSSDKVGPICRSAEDAAFVFAFIHGGDSVDASSRTMPFNYTG
                     RIALAGLRVAYARNYIDSLPDDSPSKQALAALRKMGVTVIPVDFPDDLNGNDLLSLII
                     GVESAAAFDKLTRTNLDDEMVQQNKDRWPNTFRTARFVPAVEYVNACRMRTAIMRKMG
                     PFMRQYDIIIAPPETGDQLAITNLTGNPSVTLPVGYNKDGTPGSISFIGQLFGEARLL
                     AFARAFQDATPYNKQHPKGFE"
BASE COUNT         2589 a         2879 c         2755 g         2313 t
ORIGIN      
        1 cgcgggcatg ctgaagaccc catcacatac gctggtcggt ctcgatctgc cgaacagcca
       61 tgtgcaggtg aaggacatcc tggtctttgc accccagctg gccagccagc cggtattccg
      121 caacccgaat gatgtctggc aggtcaacgg gaagatcagg gggaccttcg acgtcctgga
      181 tgttcagcag ttgcagttca gcggtataag agacttacgg ctggacatgg cgggagtgat
      241 ccggcatccc ttcgacagcc ggcgaataca gggggatatt aaccttcgca atttgtcggg
      301 cagcaggacg gcactcataa gtcttgttcc cagggggacc ctgccttctt ccatccggat
      361 cccggcccgg tacgatctgc acggacggct ggcggggagc atggatgcga tcactaccga
      421 tctgacgctg cacacttctt ccggcaccgt gatcgtcaag ggatttgcac ggaatttccg
      481 gtccacgacg gccgcaagct acgacatcgc ggtgaggaca agggcgctcg atctgggtac
      541 gatcctgcag gacagcgcac agtggggagc cgttacatcg gactttactc tacgcggtac
      601 ggggctcgac atacatagcg cgaagagccg cttttcaggc aggataacgg cagctacctt
      661 ccggcattat cattatcatg atctgacgat cgacgggtcg gtggccgatc agcaggcgac
      721 actgcactcg gcgatagaca atactgcggt cagcttcgag ctacaggctt cggccgacct
      781 atcccgtaag tttcccgccc tcaggctcga ctggcagata gacaccctgg atctccatgc
      841 cgtcggctta accacggata cgatgcaatt caaaggacat ctggcagctc agttcgaaga
      901 caccaatccc gattcgttgc aggggtctct gcgggtcacg aagatggatc ttctccaggg
      961 acaccagcac ctgatcaccg acagccttat ccttctcgcg acgcgggaat cagacaggga
     1021 acatattcaa ttgcattcgg agatggccga tctcgatctg gacgggcatt acctgttgac
     1081 gcaggtacct accgctctcg aacatacgat cagccggtat tatcatttag aaggattcag
     1141 agacaccgcc ttcgcccccc aggactggac cctgcggatg acgctaagaa cctcgccgct
     1201 ggtgctggcc atgatgcctt cgctcagggg gaccgatacg gtagggggtg tcatcgcgta
     1261 taacagtgac cgtgacgatc tgcaactggg gatcaggacg ccgcggatcg atcttggtac
     1321 ccagttcatc cataatgtag cgatcaccgc cggcaccggc aacgggcagc tgcaatatgc
     1381 cgtaacgatc tcggatgccc atggcagcgg tttggtgctg tataagacct cggtggcggg
     1441 cgcattgaag gacgataaat tgacgacgac gcttttgctg aaagatgtca agggaaagga
     1501 tcgttaccgt ctggcggggc agctggaaaa actatccggg gggctgaaac ttcatttcga
     1561 tccggatagc ctgatgctga actacgaccg ctggcaggta agccgcgata acttcatcca
     1621 atacgattcc agcggcatcg tcgcccgcga tttcaccatt agcaacaatg gggattcgct
     1681 tagcctcaac agccggggga cgaccggcgc cgcacccctc gatgtcagct tcggtcattt
     1741 caggctcagc accatcagcc gtctcgccaa ccaggactcg gtgattgccg acggcatcct
     1801 gaatgggaat gccgaggtca ggaacgtcac gacaaatccc ttgtttactt ccgatctgaa
     1861 aattgagaac gtgtcttata aggccgacac tttaggcgat ctcactttga aggtcaataa
     1921 taaaaaggcc aatgcctttt ctgcggacat cgccttgcag gggaaaaata acgatatagc
     1981 tatctcgggc gactattata caggcgaaag caagatggat ctcaaactcg atctccggcg
     2041 catcaatctt gcggccttta ccggcgcggc ccagggcgtc atggataaaa tgaaggggta
     2101 cctggcgggc cagtttacca ttgccggcac attcgataag cccaatgtcc gcggtaatct
     2161 ttatttcgac agcaccgtgg tcacacccac tatttccggc gaaacgctcg acgtatccaa
     2221 ggaccggatc gcctttgacg aggatgggtt caacttcagc gagttcactc tgaaggattc
     2281 tgccggcaat aagctgatcc tcgatggcaa cgtctttaca aaggactatc acagttttgc
     2341 tttcgatatc tctctgaatg cacagaactt ccggttgatc aatgcgccgg aaaacagctc
     2401 gcggcaattt tatgggaagc tcaatcttga cgcggccatc aacctggaag ggaagatgga
     2461 ggcaccgaag atcgacggca ccatccgggt aaacaaaaac actaatttct attatggcct
     2521 gcctcctgac aatcctgagg tcggcgatcg tgtcggcata gtccgcttcg tcgaccatcg
     2581 caccggcgat acgctcgtcg acaaaaaggc gctggcgctg aatgccaaaa agacggagat
     2641 aaaaggtctc gacgtgagcc tgaacctgct taccgatacc gccgctttgc tcaacatcgt
     2701 catcgaccct cgcagcgggg atgaactgaa tgtccgcggc cgtagcaatc tcgtttttca
     2761 gatggaaaaa agcggcaaga tggatctcac gggttcctac caggtgaacg gcggttatta
     2821 taccgtttcc ttcgaggtgc tcaaacgcaa attcaatatc aatccgaaca gcattattac
     2881 ctggacgggc gatcccacca aggccaccgt cgaccttacc gcctcctaca ccgcgatgac
     2941 accttccatc gatctggtgt ccaacgtaat tggcgatctt cccacgaccg agcaaaataa
     3001 attccggcag aaattgcctt ttctcgtgac gctaaagatg gaaggcgaca tgcttaagcc
     3061 gacgatcacc ttcgatgtct cactgccgac cacgaccctc acgctctggc cggatgtaga
     3121 ccagcgtctc accgagctgc gtagccagcc atcggagatg aataaacagg ttttcgccct
     3181 gctgctcctg ggccgctttg tcggtgagga cccgttgaac tcgcaggcgg gcggaggctc
     3241 gaccatcggt aatcttgctt tctccagcgc cagccagatc ctcaccaacc agatggacca
     3301 gattgccgcc agtctaatca aagaagtgga tatacacttc gacctcaata accagcagga
     3361 ttggaccacc ggcaatgaga tcgactacac cgaactggac gtcacggttt ccaaaacgct
     3421 gatgcacgac cggcttcgcg tcggggttgg cagcagcttc gatgtcgtcg ggaccggtgc
     3481 gccgcaacag gcgccgtcca atctggccgg gacggtcgac gcgtcctaca agctttcgaa
     3541 agacggtcgg tatctcctgc gtgtttatcg tcagaatcaa tacgaggccg tagtgctggg
     3601 acaggtgata gagacgggcg taggctttgt atttaccgtc aattacgata agctcaggga
     3661 gatcttccac cgcgccaagg gcgatcagat acaaccgact aaaacgacaa cccccaagac
     3721 aacttctcca aaatgacagg cattcggcgc atgctccggc tttttttatc gatgggactt
     3781 ttctggagtg cccttgctgc ctgtaatgaa tcgaaatatc ttgcggggaa cgagacgctg
     3841 tactcggcta acaagaccaa ggttgagagt tccgtcgcta tgtcgaataa agaaaaaaag
     3901 cggctggccg atgacgatat ggaggattac ctgcggccaa agctcaacgg aaagatcctg
     3961 ggtctccggg tcaaactctg ggtatataac attgccggaa cgacgcataa gacgaaaggc
     4021 tttaaacact ggcttaaata caagataggt gaaccgccgg tgctggcgtc accggccatc
     4081 cttgccaata atgcgcaggt gctgcagagc cacttggaga acaaggggtt cttccatgat
     4141 acggtgatcg tgaccacctt tgtaaaggac aaacacctga ccgccaccta taccgcgcag
     4201 atcggcccac gttataccat cgggaagctc gactaccccg atgactcgga tgacgtcagc
     4261 aaccggatcg attcgctgaa aaagcgctca ttgctgaaga caggcgattt cttcgatctt
     4321 gagaccatca aggaagaacg cgtcaggatc gacgaccggc tcaagaatca cggcttctac
     4381 tatttcaacc ccgatgatct gatcgtcgat ctcgatacgg ctgtcgggga tcacaaggtc
     4441 gacatgttca tgcggttgaa ggacgatatg cccgaccccg cccgcaaagt gtacaggatc
     4501 gatagcatca cggcctatgt caatcccgcg ggcaaagccg ataccaatcg aagccatgcc
     4561 tatatcactc cggaaggtta tcggatcgtc gattccgccc attatctccg gccacacgtt
     4621 ttcagcagga ccctgctgat caagccgaag gacatctata aggaaaatga tcacaattca
     4681 tcactgagcc gcctggtaag cctgggtgtt ttccagttcg taaaagcgcg tttcgagcct
     4741 gccacatcgg cggatacgaa caagatgctc aatgtttatt attatcttac tcctgcccaa
     4801 tcgaaacagc tgcgattcga ggtcacagct ctcacgcgga gcgataacac gacaggtacc
     4861 gaagtcgcgc tcgattggcg gcatcgcaat atcttccatg gcggagaatt attcagtatc
     4921 cgtgtctatg gcggtctcga aggtcagtcg gtgagtacga ccgaaaaatc attgacaaga
     4981 cgcaccggcg tcgatgctac tctcaccatt ccaaaaatcg ttgctccgtt caagctgaat
     5041 acgagcggta agttcgtgcc taagacaaag atcgcggcgg gctatgatat ctttgaaaat
     5101 aattcgttgt atacgcttac ttcctcgcgg gtgagctttg gctaccagtt caagaaccgt
     5161 gtgacgtcgg agaatggcat taccgtgttg ggaataacgt atgtccggcc ctccaatatc
     5221 accgatagtt tccaggccgc catcgataag aaccacaatc tgtattatgc catcgaaccg
     5281 cagctcatca tcggcccttc ctggaacttc aactatagct ccgtcttcga tccgcgtcat
     5341 gcccgccggc caaacaatta ttatttcaat gccaatgtcg atttttccaa caatctcctc
     5401 ggcctcattt cgggagccaa tttcaacagg accggtcatc ggaaggagat cctgggcgtg
     5461 ccgtgggcgc agtatatgcg gttcgaagcc gatttccggc attatctgaa cttcagcaag
     5521 tataatatgc tggtgagccg tttcgacgcg gggatcggta tcccgtatgg gaatagtgcc
     5581 agtcttccct ttgtaaaggc gttctttgcg ggagggacca atgatatccg cgcctttcgt
     5641 gcccgggcgc tcggtccggg acattactac gcaggcgatc ctaacgtaaa tgccttcata
     5701 ccggaccagc cgggtgacat aagactggaa ttcaacctgg aatatcgttc taatctgtgg
     5761 ggtcttgtcc gatgggcggt ctttaccgat tggggcaatg tctggacact tcggaacgac
     5821 cccaacaggc cgggtgccgt ctttacatcc gcttttttga aggatttcgc cgtcggtgcc
     5881 ggcgccggcc tccgctttga tctcaccgtc atggtgttac gtatcgatgt cgccgttccg
     5941 ctgcggacgc catggaagcc agaggggtcg aagtggaatt ttcatagcgc gacggatgtc
     6001 agcgatatgg ttctcaacct ggccatcggg taccccttct aagccgttgg tcccgctgct
     6061 tcctcaaaaa gggaatcttt tccccgcctg ttcttcccgc gtaaaatggc attatttttt
     6121 cggccgttct tattgataac ctatttgcaa acgatttaaa tacggagtta tgaaagacga
     6181 gaagagcaaa cagcagcggc agcaaccgca ggaaaacgat atggggcaaa agaaaagctc
     6241 cgatcagcaa aatcagggaa aatcttcctc cgatcaccag tcatccggga aacaaaagcc
     6301 gggacaacag ccgacaggac aacatcccac cggtgaagat ccttccagga agtctcaaca
     6361 acaaaagggt ggtatgggac acccgatgga tgtcaaagaa ggagatagga gaggggatat
     6421 cgagaatccc gatcagcagg acgaaccggg taaaaaggta aacatcgatg acgacccgga
     6481 tcagacgaaa aagaaagtgc ccaacatgca caagtagttt gtctatcccg atacgacggg
     6541 cgtcgtatcg ggatattaaa accttttttt atgaagtcga agttatatga acacgaggtg
     6601 aaaggtccag cgaggatgac aggacggaat aaaaaaatct tgagcatact ggatgacctt
     6661 atcaggatca atatcgaccg tattacggga tacgaaaaag cggcgcatga agagaacacg
     6721 ttcgaccccg aatggcgcgc caggttttat cgattggcca ccgacagcag gtcttatgtc
     6781 aacaatctgc atgcggaagt gatccgtctg ggagggcctc cggtcaccca ggctaccatc
     6841 agcggaaaga tacatctcca ttggctggaa ggaaagaacc gcttcgaagg caaggatttc
     6901 ctttcccgtc tcgcagcatg cagggcggcc gaaatgtctg tccagacagc ctatcaccag
     6961 gctttggatg aacccgagca tctgccgccg gctatttacg accttttaga gacccagtta
     7021 tgggcgcttg aaagggcgta ccaggcattc attacgcttg tttaaaagag aaattcccca
     7081 ttttttgccg aaataacagg accccgtaga aacggggtcc attatattat tctttcaggt
     7141 ttattgacta tatagatgtt aaaaatatta tgcccgaatg gaccgtcttg cacggtgatg
     7201 ctcatgggca attaattcct caaagattgc tgaaacttcg cccggggggc aaccattttc
     7261 cgtgtggaaa atctgccgcg tcacctgttt cttgctgata atgaaacgct ccggggcctc
     7321 tacttcattt ttggggcgat gtcttcccgg aacttggtat tgcttttgaa aaatgttgtt
     7381 aaaatgtcaa tctatgttgt ctcaaccaag cctgcttttt ctgcgggaaa aaatactgga
     7441 cctgaaaaac gcgattttca ttagccagca tacctcttta ctcaaaatgc ccacgaccat
     7501 cgtatccata gagaaaatgg acgagttggg tcaactgtgg ttttttgtac cccgcccgca
     7561 acaggctctc cacgaattcg accgcgaatt ccccgtgcgg ctggaattct tccggaaggg
     7621 aagggatttt tttctgcatg cttcgggtaa ggcgtttatt gtcaccgacc cggaagacat
     7681 caatggttgg ctcgaagatg acatccgcga aaaggccgac gatcgtctcg tgctgatccg
     7741 ggtaaatctc ctgaaagcag aatattttca gactccagct ccacataccc agacccgctg
     7801 gtggcgcgac ctgcgtaacc agttgcatgt ctggatcttc aatacccgcc ccggatacaa
     7861 accgtatcac ctggcagatg ctcccatgcc gatgctctcc taccggccgg tggcctgaac
     7921 cacctcttcc gtaccctcta tttatttatc cgccccgcca tggtgcgctt tccgctccgc
     7981 atggtgcgct ttccgcccgc acggcacccc ttccgccccg catggcacag gaattgaatt
     8041 cgaacgaggt gaaatcttct aaaaaacagc tatttatgaa acaattatcc atctacctgt
     8101 tatccggagc gcttactctt gcctgtttta gttgcggcaa caaccagccg agcagcacag
     8161 aggcggcaaa ggatagcaat gccacgaaaa tgagcgacag taactcaacg aatgccatgg
     8221 cttccaccgt cagtaaagcc gatcagagct ttgcggtcga tgccgccgat ggcgggatga
     8281 ccgaagtaaa ggccggccag atggccgaag aaaaaggaat ggcgaaagac gtaaaggaat
     8341 atggcaatat gatggtcaag gatcatactg aactggccga caagttgaag tcgatagcca
     8401 ccaccaagaa cattacgctc ccggggaata tctctcccga caagcaaaag gacctcgacg
     8461 atctggataa gaaaaccgga aaggatttcg ataaggcctt tttggatatg atggtctccg
     8521 atcataagaa agtcatcagc gccttccagg atgaagcaaa gaatggttcc gacgccgata
     8581 tacgcgcctt cgccgatagc agtctgcata tccttcagca ccacctggat gaggcgcaga
     8641 aatgccagaa gatggaaaaa aagatgtaag aacgtacgta cacctcttgc cataccttac
     8701 ccatgatacg gcggacagga cctccctgtc cgcttttttt gccggcgcca ggcccgccaa
     8761 aaatttttgt gaaaccaaaa ggtttcctat atttgtgtca tgtcaccgca aaacttcacg
     8821 ctctccctgc aggacggatt atttttccgg actgtcgaca ccgttggcgt ttgtttcgaa
     8881 cgcatcctcg atcattcccc ttcccgggta tgggaagtcc tgacgcaacc cgcgcacctg
     8941 tcccgatggc ttgcgcccgc ggagatcaaa ggcggtgcag gaggcgagat caggctgcaa
     9001 atgaccggtg gcaccatggg aggacgcatc ctccaatgga aggagaacgc gcttctggaa
     9061 tacatatggt acaatggtac cgtcgttcgc tgggaattac tgcaggaagg taaggaccgc
     9121 acgcggctgg tcttcacgca tacgcatgtg cagcagatcc agttgctgga cgcggccaag
     9181 ggctggcatt atcatctcga cctcttatcc atcgagctcg acggaaaaga gatcccttcc
     9241 aatccggtag aacgatggga tacgatcacc cgtgaagcca cagtccgtta taaagagcag
     9301 ctcggaggag tgcggcagac gcccccgccc ttcgtcctgg agcgcgtatt cgacgccacg
     9361 gcagatcgcg tatggcaggc cctgacgaat aaagacgaga tcaggcaatg gtcgttcgat
     9421 attgcggact tcgagcccga agaaggattc gattttacat ttgacgggga aaaggacggc
     9481 gaacgcttcg tccatctttg ccgcgttacc gaggtcgtcc ccgggaaaaa acttgcctat
     9541 tcctggcgat atgaaaatgt ccggggcgtt tcacatgtca cctgggaact gttccccgag
     9601 ggcaggaaga cacgcctccg tgtcacgcac gagggcctcg aaaaactggc gcacgcaggc
     9661 gaaccatggg cacgtcacaa tttcgaagcc ggatggacgg cgatcttcga tcagggcttg
     9721 cggtcatggc tgcagcaggc tccgatggcg ttggcgcgat agcgggcgat ggcacttatt
     9781 cgatagtgtt ggttgcttcc cggaggccgg cggcgtctat tcgaaccctt tcggatgctg
     9841 cttattatac ggcgtcgcat cctggaacgc cctggcgaat gccagcaacc gcgcctctcc
     9901 gaacaactgc ccgataaagg atatactccc cggcgtccca tctttgttat aacccaccgg
     9961 cagcgtcacc gaaggattcc ctgtcagatt ggtaatggcc agctggtcgc ccgtctccgg
    10021 cggtgcaatg atgatatcat actgccgcat aaagggcccc atcttccgca tgatggctgt
    10081 ccgcatacgg caggcattga catattccac tgccggcacg aaacgcgccg tcctgaaggt
    10141 attgggccac cggtccttgt tctgttgcac catttcatca tccaggttgg tgcgcgtcag
    10201 cttgtcgaac gcggctgccg attcgacgcc gatgatcagc gacagcaggt cgttgccatt
    10261 taaatcgtcg ggaaaatcca ctggtataac ggtaacgccc atcttccgaa gggccgccag
    10321 cgcctgtttc gaaggactgt cgtccggcag actgtcgata taattgcgcg cataggctac
    10381 ccttaatccg gcgagcgcga tccttcccgt ataattgaac ggcatcgtcc gcgaggaggc
    10441 gtcgacgcta tcacccccat ggataaacgc aaatacgaag gcggcgtctt ctgcgctacg
    10501 gcagataggc cccactttgt cagagctcca gcacag
//
LOCUS       DASYSV010000102         5279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_20456, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000102 DASYSV010000000
VERSION     DASYSV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20456"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..924)
                     /locus_tag="VGQ51_03190"
     CDS             complement(157..924)
                     /locus_tag="VGQ51_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035594.1"
                     /translation="MDKLKRYLLRHKDELDVDAPPDDAWEPIASKLSDGPYPANLSTM
                     PGFPAKPPVSGRLVRYLVAACLIGLAGIGFWLVVRDQKVPVKATANSNGAGDIKKPAH
                     ADGTRRDTIRVREEPSGKELVRNSGAPKRPRHKAGTPAPVDPSDAIGVIDNSYSSLID
                     DQLRKLRATPLYAENGDYFSFYADQLKQMDQDENQVRSDIKTFGLTSEFLEQLINVYQ
                     QKLNLLNNLRKEVNKMNNKVRGKQTPSPKAVVHYLNI"
     gene            complement(917..1435)
                     /locus_tag="VGQ51_03195"
     CDS             complement(917..1435)
                     /locus_tag="VGQ51_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV9035595.1"
                     /translation="MVMAGQGDPNAQADLYRHYSKAMYSLCIRMTGDPDDAKDVLQDT
                     FITAFRHLGQLKQPAAFAGWLRRITINKCIQFGKNNDQWSSLDQEATHPEEDQDGAWW
                     LKIPIQLVQWEIKNLPAGCRQVFVLFALENFGHRDIARNLGISESTSKTQYQRARKLL
                     RDRITKKMHLHG"
     gene            complement(1616..1867)
                     /locus_tag="VGQ51_03200"
     CDS             complement(1616..1867)
                     /locus_tag="VGQ51_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035596.1"
                     /translation="MTMNNLNASLDRLENHCPVCGQKRYMTNRGDREWTLHCSSVEAR
                     FWNFKTGSLAQTIARQHWVQSRLALRYRKEDHENAVLLI"
     gene            complement(2549..>5279)
                     /locus_tag="VGQ51_03205"
     CDS             complement(2549..>5279)
                     /locus_tag="VGQ51_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9035597.1"
                     /translation="RTGKVIRHYNAGKDSTSLNSNFIVTLYKTRNNDILVGTWEGMFK
                     YNREKDNFIRLPFFNTQIQAIHEDGQGVIWASTYGNGVYYFNPATGRGGRLKHDPGNP
                     NSLINDYVNGLFEDRDHNYWFSTEGGISKWDKNGRFTNMTIESGLPDNQTFRVLQDDQ
                     GRLWISTSKGLVRLDPTHNTLKLYQTSNGLLTDQFNYNSAFKNTDGSLYFGTVKGLIS
                     FNPDNFIENEIVPPVYITGIQVNNAILPIGGKCAALTKAITSAGQVRLPHDSADISFD
                     VAALSYTNPEMNAYQYKMEGFDKEWTALKTNRRIYYTRLPPGQYTFRVRGSNGDGIWN
                     SGPTSLRVVILSPVWATTWAYIGYIVLGGLLLFTILWYYFKALRARNKRKIDIFEREK
                     EREIYNSKIEFFTNIAHEIRTPLTLIKMPLDKLLDQESMSYSTTECLHMIKKNTSRLI
                     DLTNQLLDFRKAEANHFSLNFTKTDINVLLEEMIALFRSAAENKKIRLTLDLPSIDLN
                     AFVDEEAFKKIVANLLNNAIKYGRTQVTVRLLPFSSEDSFFRIEFRNDGYTIPSALQQ
                     KIFEPFYRIRETSKEAGTGIGLPLARSLAELHSGTLEWKESADKTNLFLLSLPFHQKR
                     EMNLKGEDIPESPSMETQEVPGSAFDTDKPLLLFVEDNKDILAFVHKELSLFYNIIKA
                     FNGLEALELLKKEVIQLVISDIMMPVMDGIELSKKMKSDIQFSHIPIILLTARSSVQS
                     KIEGLEVGADAYIEKPFAIGHLHAQIDNLLQNRNIIKDYFARTPLAHIKSIAISAADK
                     HFIEELNKVIHDNIADMDLNVEHLSKMMNMSKATLYRKINALSDMTPNELINLSRLKR
                     AAELLSEGNHKISEVSCMVGYTLQTNFTRDFSKQFGVTPSNYILRLKNEQLKA"
BASE COUNT         1178 a         1274 c         1386 g         1441 t
ORIGIN      
        1 ggatgacatt accaatgaat accgtcgccg ttgcatcgca ggggagctcg tccgtaactt
       61 ctattaattt gcgttcctgg gcacgtagtc cgccgccgat catacacagc aaaaggcagg
      121 ttgtcggcat gagcctattt cttgtcatag cattgtttat atattcagat aatgaactac
      181 ggcctttggt gatggagtct gttttcctct aaccttatta ttcattttgt taacctcttt
      241 ccgcaggttg ttcaacaggt tgagcttttg ctggtataca ttgatcaatt gctccaggaa
      301 ttcgctcgtg aggccaaagg tttttatgtc gctccgcact tgattctcat cctggtccat
      361 ctgcttgagt tgatcggcat aaaatgaaaa atagtcgcca ttttctgcat ataagggtgt
      421 cgcgcgtaat tttctcagtt gatcatcgat gaggctcgag tagctgttgt cgatgacgcc
      481 gatggcatca gatggatcca ccggagcagg tgttcccgcc ttgtggcgcg gccgttttgg
      541 tgcaccggaa tttcggacca gctctttgcc ggagggctct tcccgtaccc gaatggtatc
      601 tctgcgcgtg ccgtccgcgt gtgccggctt ttttatgtcg cccgcgccgt tggaattcgc
      661 cgtggctttg accggcactt tttggtctct gactacgagc caaaatccga tgcctgcaag
      721 accgatgaga caggcggcca ccaggtatcg caccagccgt cctgaaaccg ggggttttgc
      781 cgggaagccg ggcattgtcg aaaggttggc tggatacggt ccatccgaca attttgaggc
      841 aatcggttcc caggcatcgt ccggcggagc atctacatcc agctcatctt tgtgccgcag
      901 caggtatctt tttagcttat ccatggagat gcattttttt tgttattcga tccctcagta
      961 atttcctggc gcgctggtac tgggtcttac tggtgctttc cgagatgccg aggtttctcg
     1021 cgatgtcccg atgtccaaaa ttctccaggg cgaacagcac gaatacctgt ctgcaaccag
     1081 cgggtagatt ttttatctcc cactgtacca gctgaatcgg tattttaagc caccaggcgc
     1141 cgtcctggtc ctcctcaggg tgggtcgctt cctggtccag gctactccat tggtcattgt
     1201 ttttcccaaa ctgaatacat ttattaatag tgatccttcg gagccatccg gcgaaggctg
     1261 ccggttgttt taactgccca aggtgccgga atgcagttat gaatgtatcc tggaggacgt
     1321 cctttgcgtc gtcagggtcg ccggtcatcc ggatacaaag gctatacatg gccttgctgt
     1381 aatgccgata gagatcggcc tgggcgttag ggtcgccctg ccccgccatg accacagtca
     1441 attcatgtat gttgggtgtt tgctgcaagt ccgtagtccc atcaaaaaga gatatgaaat
     1501 ggctaaaagt tgcaagggac cggaaaaaat attttactca gtgtgattcg gctaaagcgg
     1561 gacgagacat ggcgccgtcc ctccggtggc tgcgtcggca ggaactacaa gctgtctaga
     1621 tcagtaacac cgcgttttca tgatcttctt ttctatagcg aagggcaagt cgagattgga
     1681 cccaatgttg tctggcaatc gtctgagcga gactgccggt tttgaaattc cagaaccggg
     1741 cttccacaga cgaacaatgc aacgtccatt cccgatcgcc gcggttggtc atatagcgtt
     1801 tttgcccgca gaccggacaa tggttctcca acctgtccag ggaggcattc agattattca
     1861 tggtcatgct gcaagtattg acgagcgtcc cggcgccggc ccgatagccg tccgccgctc
     1921 ccctaccagg aagaaaactg gatttatgtg gcaaaacagc ccggcgggga cgctctgagt
     1981 aaatgaattt tcggcctaag gacggctgaa gtgttcagac cgatgaaacg tctgcatcag
     2041 ccgttaaaac ttggagaaag gagggcccct caggtaagcg gtgaggcatg aagccgtcgc
     2101 ataaaagccc gaccggccga cggtaattac tgctacccgt gttctcagcg cagcgatatc
     2161 catcaggggc ggctgcaaca aatcgaaatg agccgtttga cgtaaatgcg tttgggaacg
     2221 ccccctgtcg tagcccaaat gggtccgctc atcgaccacc cgacgggatt ttttcagcga
     2281 caggcttgtt tgtatgcgtt gagcactcac acgcccgtac gactgaacag aagcctgcgc
     2341 agcgaatacc gtaaagcaaa tcgccgtgag aaaggcggat gcagtccagc gaagtaggtt
     2401 atgttttaga gttaacatgt cctgatggct tcgtttatac gagcatttac cgtttgaaat
     2461 tagttcaaat cccggtgaat agaaaaacgg ccgtcacaaa gttttgtcaa agtaagattg
     2521 taaggacggc aatgtgccat ggcctcttct acgccttgag ctgttcattt ttcaaccgga
     2581 ggatatagtt ggacggcgtc accccgaatt gtttgctgaa gtcccgggta aagttcgttt
     2641 gcagggtata gccgaccatg caggaaacct cgctgatctt gtggttgcct tccgacaaca
     2701 gctcggccgc cctcttcaac cgggagagat tgatcaattc attgggtgtc atgtccgaca
     2761 acgcattgat ctttctatac agggtcgcct tgctcatatt catcattttc gagaggtgct
     2821 ccacattcag atccatgtcc gctatgttat catggatcac cttattcagt tcttcaatga
     2881 aatgcttgtc ggcggcggag atagcgatgc ttttaatgtg cgccagtgga gtgcgggcaa
     2941 aataatcctt tatgatgtta cggttttgaa gaagattgtc gatctgcgca tggaggtgcc
     3001 cgatggcgaa gggcttttct atataggcgt cggcgccgac ttcaagtccc tctattttgg
     3061 attggacgga ggatcttgcg gttagaagaa taatagggat atgactgaac tggatgtcgg
     3121 atttcatttt tttgctcaat tcgatcccgt ccatcaccgg catcataata tcgctgatca
     3181 cgagctgtat gacttccttt ttcagcaatt cgagggcctc cagcccattg aaggctttaa
     3241 taatattata gaacaggctt aattccttat ggacaaaggc caggatatcc ttgttatcct
     3301 ccacgaacaa aagcagcggt ttatcggtat cgaatgcgct gccgggtact tcctgcgtct
     3361 ccatggacgg agattcgggt atgtcttcgc ctttcaggtt catttccctt ttctggtgga
     3421 aggggaggga cagtaagaat aggtttgtct tatcggccga ctccttccat tcaagcgtgc
     3481 cgctgtgtaa ttcggcgaga gaccgggcga gtggcaggcc gataccggtg cccgcctctt
     3541 ttgacgtctc cctgatgcga tagaagggtt cgaagatctt ttgttgaagc gcggacggaa
     3601 tggtgtaccc atcgttcctg aattcgatgc gaaaaaagct gtcttcactg ctaaaaggca
     3661 atagcctgac ggtaacctga gtcctgccat atttaatggc attgttcaac aaattggcca
     3721 ctattttttt aaaagcctcc tcatctacaa aggcgttgag gtcgatgctg ggcaaatcga
     3781 gtgtaagccg gatctttttg ttctcggctg cggacctaaa aagggcgatc atttcctcca
     3841 gaaggacatt gatatccgtc ttggtaaagt tgaggctgaa gtggttggct tccgctttcc
     3901 ggaagtcgag caactgattg gtcaggtcga taagacgact ggtatttttt ttgatcatat
     3961 gcagacactc cgtagttgag tagctcatgc tttcctggtc gagcaattta tccagcggca
     4021 tttttatcaa ggtcagggga gtccttattt catgcgcgat gttggtgaag aattcgatct
     4081 tggaattata aatttcccgc tctttctccc gttcgaatat atcgattttc cgcttattcc
     4141 gggcccggag ggccttaaaa tagtaccaca ggatggtaaa caggagtaag ccgcccagga
     4201 cgatgtaacc gatataggcc caggtggtgg cccagacggg ggacaggatg acgacccgta
     4261 aactggttgg tccgctattc cagatgccgt cgccattgga ccctctgaca cgaaaggtat
     4321 attgccccgg cgggagtcgc gtatagtaaa tcctgcgatt ggtctttagc gcggtccatt
     4381 ccttgtcgaa cccttccatc ttatactggt aagcattcat ttcggggttg gtatagctta
     4441 gggccgcgac gtcgaagctg atgtcggcgc tatcatgcgg aaggcggacc tggccggcgg
     4501 aggtgatcgc ctttgtcaat gccgcgcatt tgccgccgat aggaagaatt gcattgttta
     4561 cctggatgcc tgtaatgtac accggcggta cgatctcatt ttcgatgaaa ttatccgggt
     4621 taaaactgat caaacctttg accgtcccga agtaaaggga gccgtcggta ttcttaaatg
     4681 ctgagttgta attgaactgg tcggtcagca gtccattgga ggtttgatac aatttaaggg
     4741 tattatgggt aggatccagc cggaccaggc ctttggatgt agagatccat aatcggccct
     4801 ggtcatcctg aagcacgcgg aaggtttggt tgtcgggcag gcccgattca atggtcatgt
     4861 tggtgaatcg gccattctta tcccatttgg agatgccgcc ttccgtgctg aaccaatagt
     4921 tgtggtcacg atcctcaaaa agaccgttga catagtcatt gatcaggctg ttcgggtttc
     4981 cgggatcgtg ttttaatcgg cctccccgtc ccgtcgccgg attgaagtag taaacgccat
     5041 tgccataggt gctcgcccag atcacgccct gcccgtcctc atggatcgcc tgtatctggg
     5101 tgttgaaaaa gggcaaacga atgaaattgt ctttttccct attatacttg aacattcctt
     5161 cccaggtgcc gacgagaata tcgttgttcc ttgttttata aagggtgacg atgaaattac
     5221 tgttcaggga agtagagtcc ttaccggcat tgtaatgccg aatgaccttt ccggtgcga
//
LOCUS       DASYSV010000103        14820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1428602, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000103 DASYSV010000000
VERSION     DASYSV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428602"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2240
                     /gene="nirB"
                     /locus_tag="VGQ51_03210"
     CDS             <1..2240
                     /gene="nirB"
                     /locus_tag="VGQ51_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEV9035598.1"
                     /translation="QARVVHSLRGLSQPYDVLVLATGSSAFVPDIVGIEKEGVFVYRT
                     IEDLERIKAYAPRAKSGAVIGGGLLGLEAAKALLDLGIPRTHVIEFAPRLMPRQIDSA
                     GSAALEASLRKLGLQLHLNRVTQSIEGNGRVEGLQFADESRLAVDMLVISAGIRPRDE
                     LARLAGLSVGTRGGILVNDKLQTSDKHIYAIGECALSQGMIYGLVAPGYEMADVVATN
                     LAGGNKTYTGFDMSTKLKLIGVDVASFGDPFAAEPDCKSIVFENRHKGVYKRINISSD
                     GKYLIGGVLIGDAGAYNLLLQTVNNRLVLPPDPEDLILGSRDGSSEGAGVMHLPDEAL
                     ICSCESVTKAAICSAVTEQGVETLDGVKKCTRAGTGCGGCIPLVKDLMTGTMKAAGVY
                     IRNTLCEHFTKSRQELFDLIRLHDVHTYEDTLKKWGKGDGCEVCKPAVASILASIWNE
                     NIAKQATIQDSNDRYLANIQKGGSYSVVPRIPGGEITPDKLIVLGKVAKKYKLYTKIT
                     GGQRIDLFGAHVGDLPSIWEELIEAGFESGHAYGKALRTVKSCVGSTWCRFGLHDSVS
                     FAIGIEERYRGIRAPHKIKGGVSGCIRECAEAQGKDFGIIATEKGWNLYVCGNGGSRP
                     QHAQLLASDIDAASCIRYLDRFLMFYIRTADPLTRTATWLNKMEGGMSYLRNVVINDS
                     LGMGAQWEREMQGLVDSYKCEWKEVVENPELRKRFNHFVNAPRKKDPALEFDDLRGQI
                     KAREW"
     gene            2258..2620
                     /gene="nirD"
                     /locus_tag="VGQ51_03215"
     CDS             2258..2620
                     /gene="nirD"
                     /locus_tag="VGQ51_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEV9035599.1"
                     /translation="MNTTTTAERVKWINACRVEDIPADGGACVLLGGEQIAIFHFKRR
                     GEWFATENRCPHKMQMALSRGMTGSAGEQCEPKIACPFHKKTFSLRTGECLSGEEYKI
                     RTFPVQVKGGKVYVGMMA"
     gene            2631..3581
                     /gene="moaCB"
                     /locus_tag="VGQ51_03220"
     CDS             2631..3581
                     /gene="moaCB"
                     /locus_tag="VGQ51_03220"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HEV9035600.1"
                     /translation="MRDISHKQITLRTARASGIVFCSPATIEIIRNGQLPKGNLFDVA
                     RAAGFLGAKHTPQLLPHCHPVTIDGMEMEFEIPGPGTGATPGDEWPVGRTGILITAEA
                     RSIGRTGIEMEVLTGVAVAALEIYDMLKPVDRQLEIGHIRLLEKRGGKSDRERYFATA
                     PTCAVMVCSDSTAEGKREDKSGRIIQDMLTAAGALVVSYVVVPDEKTVIQQQVRQWVD
                     EDIQFIFTTGGTGLGPRDNTVEAVSSILERDADGIAEAMRAYGQQRTPMAMMSRGVAG
                     SIRHTLIVTLPGSSDGAKESLEAILPAVFHARKMMKGGGH"
     gene            3613..4857
                     /locus_tag="VGQ51_03225"
     CDS             3613..4857
                     /locus_tag="VGQ51_03225"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEV9035601.1"
                     /translation="MLINYRQAREIVLSQARSFGREEVSLEQAFGRVLSETVRADRDY
                     PPFDRATMDGYALRYADLELGIRRFRVAGVLYAGGRPEMNTVIGAGECFRIMTGAAVP
                     APADIVIRRENVKEGDPFVELPGTAADLPSGVLPAPADPSFPWRPWQNIARQGEDLAA
                     GEPAIEDACVCEPAVIGLLATLGRAGVTVERLPRVSILTTGNEVVPVGVPVGPVQIRN
                     SNRWQLEAALRKGGIVPAVCTHAPDDPAALHDQLQTLIDDDLLILCGGVSAGDADHVP
                     AALESVGIKKLFHKIAMRPGKPTWCGVTPSGRMVFALPGNPFSTFVNMTLLIQPFLQA
                     CFGLPPSGPMGVPLGVERKKRSPLDEFFPVYVHGSPVRAEPVALNGSGDIRLGRQANA
                     LALHPAESGALAAGAEVPCYLL"
     gene            4972..6255
                     /locus_tag="VGQ51_03230"
     CDS             4972..6255
                     /locus_tag="VGQ51_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9035602.1"
                     /translation="MLPENQPLYRIRLFSLGMVQMRTFHITWLMFFVCFFGWFGLAPL
                     MPTIRADLGLTRSQVGNTIIASVSSTIIARLLIGRLCDRIGPRKTAVRLLMLGSIPVL
                     LVGLATNYSTFLLFRLAIGVTGASFVITQSHTSLMFAARVRGTAGAVTGGWGNLGGGV
                     TNMVMPLIFSAIVGFGYIPHMAWRYAMIVPAIMMWVVAFLYYRYTKDTPAGNFSDIGR
                     KTVARTTDWSVLADWRVATLALAYAMCFGMEITFDNVAALHFVDHFHLSQHSAGFWAG
                     MFGCMNLFARALGGIFSDKAGARWGMRGKGLLLTCALLLEGIGLIAFAQAPGFAVAIV
                     CMLSFALFLKMANGATYGIVPFIDEKNVGLISGAVGAGGNLGGMIFGFLFRSENITYS
                     QAFTCIGVMILLAAGIVALVRWTKSDAVTTTPVTA"
     gene            6264..9845
                     /locus_tag="VGQ51_03235"
     CDS             6264..9845
                     /locus_tag="VGQ51_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV9035603.1"
                     /translation="MSGPPRHTERFHSTCCYCGVGCGVNVYKDRKGMITVEGNPDYPV
                     NKGMLCSKGLNLHYTVNDRRDRLLYPQMRYNRQMPLRRVGWDTALDRAAAVFKSLIGR
                     YGPDSVGFYISGQCLTEEYYVANKLMKGFIGSNNIDSNSRLCMSTAVAAYKMALGEDS
                     VPLSYADLELADCFFLSGANPAWCHPILWRRIEAHKAAHPAVKIIVADPRRTDSSAIA
                     DLHLPLNPGTDVTLHYAIGRLLIENGMVSTAFIREHTEGFEDYRVRVFERTVEEAAAI
                     CGLTAESIRQAAEWIGGAGGYISLWTMGLNQSVTGVDKVLSLIDLHLSTAHIGRPGSG
                     PFSLTGQPNAMGGRETGGLANLLPAHRDLSNPAHRRAVEDHWRSGPISPRPGLTATEM
                     FEAMADGRLKAIWIMGTNPLVSLPDVRLAEAALKKARFVVVQEVSTKPETLAYADLIL
                     PAAAWTEKQGTMTNSERRISLLDKIVEPPGEALPDGVILCRLAQKMGFTGFDYAGPAD
                     IFREHALLTFGTSIDISGLDYDILRERRSVQWPFPAGGQELGTGGHGSGPGLEQQGSG
                     PGLGQHGSGLDPVRLFTGGRFPTPTGKARFHAPADGLRSELPDQDFPLVLTTGRVRDH
                     WHTMSKTGKVARLRQHAPQSILEMHPEDACHLGIGDGDIVVVESRRGTVRVPVRLTAN
                     VKKGSVFMPMHWGTIQGSDLNRVNNLTHNLLDPVSKEPDLKFCAVRVTRYQKRKETIV
                     IVGAGAGAHAFVRSYRALNGEDEIIVFNKEDLPFYNRVQLPDYISGTRHWEQLVRMTD
                     EEEIDCDIRLHRGVSIELIDREKQQVKDSRGAVTGYDKLILAMGSRAALPRQVPELEG
                     IFTLRSRGDADRFRQRLETGSRVIVVGGGLLGLELAASLREMDIGVSIVHRSSRFLDR
                     QLDHLGSQLLHDEMIDQGCIIYYSDEVQLYYGQTRLTGIRLRSGRVLECDALVFAIGT
                     LPNIEIAKTAGLEHRRGVVVDGYLRTTDPNIHAIGEIAEFEGGLYGFTAAAEQQAEAL
                     ARYLSGDIARPYTGTVPMNIIKIHGFDLCSIGLPECPDARDYEEIVFIDKARRYYKKC
                     IIYQDRLVGAILIGDKDEFREFREWITGGIELNERRLQLLRSGTRVQPVLGRLVCSCN
                     NVGTDNLRQKIAEGCSDLAALCSVTGAGTGCGSCRPEVKRILEEHVS"
     gene            9835..11316
                     /locus_tag="VGQ51_03240"
     CDS             9835..11316
                     /locus_tag="VGQ51_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEV9035604.1"
                     /translation="MFRKNYIVKLNLPGGIVAAGDLYAITEAAERARVEDMQLGGRQQ
                     LFCKVADRYGEAFLRELDAAGIFYETEKERYPNIISSYVTEGIFEKSQSQWVSEGLYK
                     DILEGFDFRPRLKVNLVDIGQSFVPFLTGHLNFISSAVNNHWYLVVREPGTNRMYTWP
                     TGIYSPDIPAISRAMEKVLLDGSGGEIRSAAGSRGQQMYEAVRGSGNFREGPVLPEMR
                     LPPFQLPYYEGFNRAGNKSWLGIYRREELFPLAFLKDVCLLALQTKNGQLYTTPWKSL
                     LIKNIGDPDLSRWEFVLGRYRMNVRHASNELNWQIEDLCPDGLRLKRYLVRQMDQDDV
                     RTEGLSFTIKTQKGSGLAGSVVIRLQEGARSGQHKVLDRYSIWHTEEFDAHARESVLY
                     RKDLEKETLFPYLVSLCKAFYEQQSGDPILAVEPGTGIGAIARQEPGFQCRHCLTLYE
                     EQYGDPDQAVLPGTPFGRLGADYVCPVCGAPKADFDASVVTLG"
     gene            11319..12284
                     /locus_tag="VGQ51_03245"
     CDS             11319..12284
                     /locus_tag="VGQ51_03245"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEV9035605.1"
                     /translation="MMAPIKDDYGRTFRTLRVSLLQHCNLGCVYCVAGEDQLKQANAS
                     PAGGSLAVGELLAIIRRLHGQLELDHVRLTGGEPLLYPELPALIRGIRDAGIASVRMT
                     TNGFLLERQAASLKEAGLEALNVSLDAVDEEVFFRISRRRSAVRIIKGVDAALQAGLA
                     VKLNAVVMKGINDSEILPLLDLAISRGIPIRFLEVMAMGHLHADADRYLFPQEEILRV
                     IGARYPCEPLPRAAGATARYWKTENGHVFGIIANESEPFCRDCDRLRLDSSGKIYGCL
                     SSNHPIGLGVDEGDDEWRDKLQRALSQKQALRFVGSDLSMLRIGG"
     gene            12298..12531
                     /locus_tag="VGQ51_03250"
     CDS             12298..12531
                     /locus_tag="VGQ51_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEV9035606.1"
                     /translation="MKIHVYAGLKDYFQPEFEIESFARNTDELKVLLLAINDKAEKML
                     SSCRFAVEEGFIDNEFKLNEHDTIVIIPPASGG"
     gene            12491..13060
                     /locus_tag="VGQ51_03255"
     CDS             12491..13060
                     /locus_tag="VGQ51_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEV9035607.1"
                     /translation="MTPSSSSRRLAGDRGAGEGGERVAAGRPGGEEAGRVGALSAGPI
                     DMAGMLAQAHHPGAGGVVLFSGEVRDSNHGRAVGFLEYEAYAPLAEKMIGEILATAKE
                     RWELHIALAQHRTGRVDVGDTAVVVITASAHRSEAYAANRYIIDRIKHEAPIWKCEHY
                     ADGGKEWGNNCNCHAVTGDVSKHIYESER"
     gene            13044..13649
                     /locus_tag="VGQ51_03260"
     CDS             13044..13649
                     /locus_tag="VGQ51_03260"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEV9035608.1"
                     /translation="MKANADAPGRSKDGRIADLLGVILCGGESRRMGRDKGLLLADGV
                     PWALHMAGKLGPWGLPVVYSINKGQYETYSAHLPGQRLVVDRLGLAGPLEGLLTVHAA
                     YPGSDLLLLACDLQDLDAETLSGLIGKYLAEEANAYAYRDADGLQPLCAIYTSVLLRP
                     RYEAVKEGRAIDVRLRSLLDGEGVRLLEAGSEGAFRNYNTL"
     gene            13935..>14820
                     /locus_tag="VGQ51_03265"
     CDS             13935..>14820
                     /locus_tag="VGQ51_03265"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV9035609.1"
                     /translation="MGEKAFVTPAVLKWSRETARMTVEQAAGKASVSAQQVVDWESGH
                     DRPTIRQARLLAEAYRRPFALFMLPAPPRDWTPLKDFRRRSAIPFTTATEFLIRDLRV
                     KQTWASEWNREQGEAPVPFVGRFTLRHSPAKVAADILKELDIHPPNYANPLKEWLTKA
                     ELKGIYISRTSYLNSHLTLDSEEFQGFAIADPMAPLIFINTDDWDTAQLFTLVHELAH
                     VWIGETGISNEVALNFKSLNNIDQVERFCNQVAGIALMPDGFVSVLPASTFTSLEEVL
                     AQARRCGVSGFALLVRARN"
BASE COUNT         3108 a         3762 c         4810 g         3140 t
ORIGIN      
        1 ggcaggcgcg ggtggtgcat tccttaaggg gtctttcgca gccctatgac gtactggtgt
       61 tggccacggg ttcctcggcc ttcgtgccgg atatcgtcgg gatcgagaag gagggagttt
      121 tcgtataccg aacgatagaa gatctcgagc ggataaaggc ctatgctccg cgggcgaaaa
      181 gcggggccgt catcggcggc ggtttattgg ggctggaggc ggccaaggcc ctgctcgatc
      241 tcggtatccc ccggacgcac gtgatcgagt tcgccccgag actgatgccc cggcagatcg
      301 acagcgcggg gagtgccgca ctggaggcca gtcttcgcaa gctggggttg cagctgcatc
      361 ttaacagggt gacccagtcc atcgaaggta atggacgggt ggaagggttg caattcgccg
      421 atgaatcgag gcttgccgtg gatatgctgg tgatctcggc gggtatccgg ccccgtgacg
      481 agctggcgcg gctggcgggt ctttcggtcg gcacgcgggg cggcatcctg gtcaatgata
      541 agctgcagac gtccgacaag catatttatg ccattgggga atgcgcgctt tcgcagggaa
      601 tgatctatgg gctggtagcc ccgggttatg aaatggcgga tgtggtcgcc acaaacctgg
      661 caggggggaa caagacgtac accggcttcg atatgagcac gaagctgaag ctcatcgggg
      721 tggacgtggc cagtttcggc gatccgtttg ccgccgagcc ggattgcaag agcatcgtgt
      781 tcgagaacag gcacaagggc gtttacaaac ggatcaatat cagcagtgac gggaaatatc
      841 ttatcggcgg cgtcctgatc ggggatgccg gcgcctataa tcttttatta cagacagtga
      901 acaacaggct agtccttcca cccgatccgg aggacctgat actcggtagc cgggacgggt
      961 cttcggaggg tgcgggcgtg atgcatcttc cggatgaggc cctgatctgt agttgtgaat
     1021 cggtgaccaa ggcggcgatc tgttcggcgg tgacggagca gggggtggaa acactggacg
     1081 gggtgaagaa atgtacacgc gcgggcacgg gatgcggcgg atgtatccca ttggtgaagg
     1141 atctgatgac gggaacgatg aaggcggcgg gcgtgtatat ccgcaacacg ctgtgcgaac
     1201 atttcacgaa atcgcggcag gagcttttcg atctcatcag gctccatgac gtgcatacct
     1261 atgaggatac gttgaagaaa tggggtaagg gcgacggatg cgaggtctgt aaaccggccg
     1321 ttgccagcat cctggccagc atctggaacg agaatatcgc caaacaggcg accatacagg
     1381 atagcaatga ccggtatctc gccaatatcc agaaaggggg cagctattcg gtcgtgccgc
     1441 gcatcccggg tggcgagatc actccggaca agttgatcgt gctgggaaaa gtggcgaaga
     1501 aatacaagct gtataccaag atcaccggtg gtcagcgtat cgatctgttc ggtgcgcatg
     1561 tcggcgatct tccgtccatc tgggaagagc tgatcgaagc ggggttcgag agcggtcatg
     1621 cctacgggaa ggcgttgcgt accgtgaaga gctgcgtggg cagcacctgg tgccgttttg
     1681 gattacatga cagcgtttcc ttcgcgatcg ggatcgaaga acgataccgc gggatccgcg
     1741 ccccgcataa gataaaaggc ggcgtcagcg gctgtatccg ggaatgtgcc gaggcgcagg
     1801 gcaaggattt cgggatcatc gccacggaaa aaggctggaa cctgtatgtc tgcggcaacg
     1861 gcggatcgag accgcagcat gcgcagctgc tggcatcgga tatcgatgcc gcctcctgta
     1921 tccggtatct cgacaggttc ctgatgttct atatccgtac ggccgacccg ctgacgcgga
     1981 cggccacctg gctgaacaag atggagggcg gcatgagcta tctgcgcaat gtcgtgatca
     2041 atgacagcct gggtatgggc gcgcaatggg agcgggagat gcagggtctc gtggacagct
     2101 acaaatgtga atggaaggag gtcgtggaga accccgagct gcggaagcgg ttcaatcatt
     2161 tcgtcaatgc gccccggaaa aaggatccgg cgctggaatt cgatgacctg cgcggtcaga
     2221 tcaaggcgcg ggaatggtaa acttttaatt tttttctatg aatacgacga ctacagctga
     2281 acgagtgaaa tggatcaacg cctgccgggt ggaggatatc cctgccgacg gcggagcgtg
     2341 cgtccttctc ggcggagagc agatcgctat ctttcatttc aagcggcgtg gcgaatggtt
     2401 cgccacggag aaccgctgtc cccacaagat gcaaatggcg ctttcgcgcg ggatgaccgg
     2461 cagcgccggc gagcagtgcg aacccaagat cgcttgtcct tttcataaga agacgttctc
     2521 tttacgcacc ggcgagtgtc taagcggcga ggaatacaag atccgcacgt ttcccgtgca
     2581 ggtgaagggc gggaaggtat atgtcgggat gatggcgtaa attcgccttc atgcgcgaca
     2641 tcagccacaa gcagatcact ctgaggacgg cccgggcatc gggcatcgtc ttttgttctc
     2701 ccgcgaccat tgagatcatt cggaacgggc agctgcccaa gggcaatctt tttgacgtgg
     2761 caagggccgc aggatttttg ggcgccaaac atacgccgca gctattgccg cattgtcatc
     2821 cggtgactat cgacgggatg gagatggaat tcgaaatccc cggtcccggc acgggtgcga
     2881 cgcccgggga cgagtggccc gtgggaagga ccggcatcct gatcacagcc gaggcccggt
     2941 cgataggaag gaccggcatc gagatggaag tcctgaccgg agtggccgtg gcagcgctcg
     3001 agatctacga tatgctcaag cccgtagaca gacaactgga gataggtcat atccggttgc
     3061 tcgaaaagag gggcggcaag agcgaccggg agcgctattt cgccactgcg ccgacctgcg
     3121 cggtcatggt gtgttcggat tcgacggcgg aggggaagcg cgaagacaaa agcggccgga
     3181 tcatacagga tatgctgacg gcggcgggtg cgctggtggt gtcctatgtc gtcgtgcccg
     3241 atgagaagac ggtgatccag cagcaggtac ggcaatgggt agacgaagac attcaattca
     3301 tctttacgac cggcgggacg ggtctcggac caagggataa tacggtggag gccgtcagct
     3361 cgatccttga gcgcgatgcg gatgggatcg cggaggcgat gcgggcctat ggccagcagc
     3421 gcacgccgat ggctatgatg agcaggggag tggcggggtc gatcaggcac acgctgatcg
     3481 taacgttacc cggcagcagc gacggggcga aggagtcgct ggaggcgata ctaccggccg
     3541 tttttcatgc aaggaaaatg atgaagggag gagggcattg agtgccgcgg ccgatgaatg
     3601 caaagtgtcg acatgttgat caattatagg caggcaagag aaatcgtatt atcgcaggcg
     3661 cggtccttcg gacgcgaaga ggtgtcgctg gagcaggcgt tcgggcgggt attatcggag
     3721 actgtccgcg ccgaccggga ttatccgcct ttcgaccgcg cgaccatgga cgggtatgcc
     3781 cttcgctatg cagacctgga gctgggtatc cggcgttttc gcgtggcggg tgtcctgtat
     3841 gcggggggga ggcccgaaat gaacaccgtc attggcgccg gggaatgttt caggatcatg
     3901 accggtgcgg ccgtacccgc gccggccgat atcgtgatcc gccgggaaaa tgtaaaagaa
     3961 ggcgatccct ttgtggaatt gcccgggacg gcggccgatc tgccttccgg cgtattgccc
     4021 gcacccgccg atccttcttt tccatggcgt ccatggcaga atatcgcgag acagggagag
     4081 gatctggctg ccggcgagcc ggccatcgaa gacgcctgtg tgtgcgaacc tgccgtcatc
     4141 ggactgctgg ccaccctggg ccgggcgggt gtcaccgtcg aacggctgcc gcgcgtgagc
     4201 atattgacga ccggaaatga agtagtaccg gtaggcgtcc cggtgggtcc ggtacagata
     4261 cgcaacagca accgctggca gctggaagcc gccttgcgga aagggggtat cgttccggcg
     4321 gtgtgcacgc atgcgcccga tgacccggca gcgctgcatg accagctgca aacgttgatc
     4381 gatgacgatc tgctgatcct ttgcggcgga gtctcggccg gcgatgccga tcatgtcccg
     4441 gcggcgctgg aatcggtcgg tataaaaaaa cttttccata agatagccat gcggccgggc
     4501 aaacctacgt ggtgcggggt gacgccttcc gggagaatgg tattcgccct gcccggcaat
     4561 cctttttcca cctttgtcaa tatgacgctg ctgatccagc cctttctgca ggcctgtttc
     4621 gggctccctc catcggggcc gatgggtgtg ccgctgggtg tcgagcggaa aaagcgtagt
     4681 ccgctcgatg agtttttccc ggtgtacgta catgggtcgc cggtacgcgc agaacccgtc
     4741 gcattaaacg gatcaggaga tatacgtctc ggaagacaag ccaatgccct cgccctgcat
     4801 ccggcggaga gtggagcgct tgcggcaggc gcggaagtgc cctgctattt attataaaaa
     4861 taaatatatt tacttttatg ggcacgtatg ccaaaaaatt ggcgattttt cgttaatttt
     4921 atcacataat cggttgttgc catatgttat tttccatcca aaacaaccgg catgctacct
     4981 gaaaatcaac ctttgtacag gatccggctt ttttcgctgg gaatggtcca gatgcggact
     5041 tttcatatta cctggctgat gttctttgtt tgtttcttcg gatggttcgg tctggctccc
     5101 ctgatgccta cgatccgggc cgatctgggg ctcacccggt cgcaggtggg gaataccatc
     5161 atcgcatcgg tgtcatcgac cattatcgcc cggttactga tcggcaggct ttgtgacagg
     5221 atcggaccgc gcaagaccgc cgttcgtctg cttatgctag gctccatccc ggtcctcctg
     5281 gtcggattag cgacgaacta ttctaccttc cttttgttca ggttggccat tggagtgaca
     5341 ggcgcctctt ttgtcatcac gcagtcacat acgagcctga tgttcgccgc gcgtgtccgc
     5401 gggacggcgg gtgcagtcac cggggggtgg ggcaaccttg gcggcggcgt caccaatatg
     5461 gtcatgccgc tgatcttctc cgcgatcgtc ggattcggtt atatcccgca tatggcgtgg
     5521 cgttatgcca tgatcgttcc tgccataatg atgtgggtcg tcgccttctt atattaccgt
     5581 tatacgaagg atacgcccgc cggaaatttc tccgacatcg gccggaagac tgtagcgcgt
     5641 actaccgatt ggtccgtact ggcagactgg cgcgtcgcaa cgctggcact ggcctatgcg
     5701 atgtgtttcg ggatggagat caccttcgac aacgtagccg cgctgcattt cgtcgaccac
     5761 tttcacttgt cgcagcacag cgcgggtttc tgggccggca tgttcgggtg catgaatctt
     5821 ttcgcacggg cgctgggcgg gatcttttcc gacaaggcag gcgcccgctg ggggatgcgg
     5881 ggcaagggat tattgctgac ctgcgcgctg ctgctggaag gcatcgggct catcgccttc
     5941 gcacaggccc cgggttttgc cgtcgccatc gtctgtatgc tctcgttcgc acttttcctg
     6001 aaaatggcta acggggccac ctatggcatc gtacccttca tcgatgaaaa aaatgtaggg
     6061 ttgatcagcg gagccgtcgg cgccggcggc aacctgggag gcatgatctt cgggttcctg
     6121 ttcaggtccg aaaacatcac ctattcacag gcctttacct gcatcggcgt gatgatcctg
     6181 ctggcagccg ggatcgtcgc tttggtcagg tggacaaaaa gcgacgcggt gacgaccaca
     6241 ccggtcactg cataaacgca attatgtccg gacctccccg tcataccgag cgttttcatt
     6301 ccacctgctg ttactgcggt gtgggttgcg gtgtcaatgt ctataaggac agaaagggca
     6361 tgatcaccgt ggagggcaac cccgattacc cggtgaacaa agggatgctt tgcagcaagg
     6421 ggttgaatct tcattatacc gtcaacgatc gccgcgaccg gctgctgtat ccccagatgc
     6481 gatacaaccg acagatgccc ctgcggcgcg tcggctggga cactgcgctg gaccgcgccg
     6541 cagccgtgtt caagtctttg atcgggcgat acggacccga cagcgtcggt ttctatattt
     6601 ccggtcaatg tctcacggaa gaatactatg tcgcgaacaa actgatgaag ggtttcatag
     6661 ggagcaacaa tatcgacagc aattcacggc tttgtatgtc caccgctgtc gcggcatata
     6721 agatggcgtt gggggaggac agcgtaccgc tgagctatgc cgacctcgaa ctcgccgatt
     6781 gttttttcct atccggcgcg aacccggcct ggtgtcatcc tatcctgtgg cgacgcatcg
     6841 aggcgcataa ggcggctcat ccggcagtaa agatcatcgt ggccgacccc cggcgtacgg
     6901 atagctccgc gatcgccgat ctgcacctgc ctctgaaccc gggcaccgac gtcacgctgc
     6961 attatgccat cgggcgtttg ctgatcgaaa atggaatggt ttcaactgct tttatccgcg
     7021 agcatacgga aggattcgaa gactatcgtg ttcgggtatt cgaaaggacg gtcgaagaag
     7081 ccgccgcgat ctgcggttta acggccgagt cgatccggca ggcggcagaa tggatcggcg
     7141 gcgccggtgg gtatatcagt ctctggacca tggggctcaa tcaaagcgtc acaggagtgg
     7201 ataaagtgct atcgctgatc gatctgcatt tatctaccgc tcatatcggg cgtccgggct
     7261 cgggaccctt ttcgctgacc ggtcagccga atgccatggg cgggcgcgag accggcgggt
     7321 tggcgaattt attgccggcg catcgcgatc tgtcgaaccc ggcgcatcgg cgggcggtgg
     7381 aagatcattg gcgtagtggc ccgatctctc cccggccggg attgacggcc accgaaatgt
     7441 tcgaagccat ggccgacgga aggctaaaag cgatctggat catgggcacc aacccactgg
     7501 tgagtcttcc cgacgtgcgg ctcgcggaag cggcattgaa gaaagcccgg ttcgtcgtcg
     7561 tgcaggaggt gagcacgaag ccggagacgc tggcatacgc cgatctgatc ctgccggccg
     7621 cggcctggac ggaaaaacag gggacgatga cgaattccga gcgaaggatc agccttctcg
     7681 ataagatcgt cgagccgccg ggagaagcgt tgcccgatgg cgtcatcctt tgccggctgg
     7741 cgcagaagat gggttttacc ggtttcgatt atgcggggcc ggcggatatc ttccgcgaac
     7801 atgcgttgct gacattcggg acgtccatcg atatcagcgg tctcgattat gacatactgc
     7861 gtgaacgccg gtcggtgcaa tggccttttc ctgcaggtgg gcaggaactg ggaactggtg
     7921 ggcatggctc cgggccagga ctggagcagc agggctccgg gccaggactg gggcagcacg
     7981 gctccggcct tgatccggtc cgtcttttca ccggcggtcg atttcccacg cctacaggca
     8041 aagcgcggtt tcatgccccg gcggacgggc tccggagtga gctccccgat caggacttcc
     8101 cgctggtatt gacgacgggt cgggtccggg atcactggca taccatgagc aaaacgggca
     8161 aggtagccag gctaaggcag cacgcgccgc agtctatatt ggagatgcat ccggaggacg
     8221 cctgccacct gggtatcggg gacggcgata tcgtagtcgt cgaatcgcgg cgcggcacgg
     8281 tcagggtccc tgttcggtta accgctaacg ttaaaaaagg cagcgtattc atgcctatgc
     8341 attggggtac gatacagggc agcgatctga atagggtgaa caatctaacg cacaacctgc
     8401 tcgatccggt aagcaaggag ccggatctca aattttgcgc ggtaagagtg acccgctacc
     8461 agaagagaaa agagacgatc gtgatcgtcg gcgccggcgc tggcgcgcac gcattcgtcc
     8521 gcagctaccg ggctctcaac ggcgaggatg agatcatcgt tttcaataag gaggaccttc
     8581 ctttttacaa ccgggtgcag ctgccggatt atatcagcgg aacgcggcat tgggagcagc
     8641 tggtacgaat gacggacgag gaagagatcg attgcgacat ccgtcttcac aggggggtca
     8701 gcattgaact gatcgaccgg gagaaacaac aggtaaagga ctcgaggggc gccgtcaccg
     8761 gctatgataa actgatcctc gccatgggga gccgggcggc gcttccgcgt caggtgccgg
     8821 aactggaagg catctttacc ctgagaagcc gcggggatgc cgatcgtttc agacaaaggc
     8881 tggagacggg cagcagggtc atcgtagtag gcggcgggtt gctgggtctt gagctggccg
     8941 cgtcattgcg cgagatggac atcggtgttt cgatcgtcca tcgcagcagc cgtttcctgg
     9001 accgtcagct cgatcatctg ggaagccagc tgttgcatga cgaaatgatc gaccagggat
     9061 gtatcattta ttattccgac gaagtgcagc tgtattacgg acagacgcgg ctaacgggga
     9121 tccgtcttcg aagcggccgg gtgctggaat gcgatgcgct tgtattcgcc atcggcacct
     9181 tgccgaatat cgagatcgcc aaaacggcag gtctggagca ccggaggggt gtcgtggtgg
     9241 acggatatct gcgtaccacc gatccgaata tccatgcgat cggcgagatc gcggaattcg
     9301 aaggtggcct gtatgggttt accgcggcgg ccgagcagca ggcggaagca ttggcccgtt
     9361 atctcagcgg ggatatcgca aggccgtata ccggcaccgt gccgatgaac atcatcaaga
     9421 tccatggatt cgatctgtgc agcatcgggt tacccgaatg tcccgacgcg cgcgattatg
     9481 aagagatcgt cttcatcgac aaagcgcgcc gctattataa gaaatgcatc atctaccagg
     9541 acaggctggt gggcgccatc ctaatcgggg acaaggatga attccgggag ttcagggaat
     9601 ggatcaccgg cggcatcgag ctgaacgaac ggcgattgca actgttgcgc agcggcacga
     9661 gggtccagcc ggtgttaggc aggctggttt gcagctgtaa caatgtcggc accgacaacc
     9721 tgcgtcaaaa gatcgccgaa ggctgtagcg atctggccgc cctgtgcagc gtaacgggag
     9781 cgggaaccgg atgtggcagc tgccgtccgg aggtgaaacg gatactggag gaacatgttt
     9841 cgtaagaatt atatcgtcaa gcttaatctc cccggtggga tcgtcgccgc aggggacctg
     9901 tatgccatca ctgaggcggc ggaaagggcg agggtcgaag acatgcagct ggggggccgg
     9961 cagcagcttt tttgcaaggt ggccgatcgg tatggcgagg cgttcctgcg cgaactggat
    10021 gctgccggca tcttttatga gacggagaaa gagcgctatc cgaacatcat cagcagctat
    10081 gtcacggagg gcatttttga aaaatcgcaa tcgcaatggg tcagcgaagg actttataag
    10141 gatatcctgg aaggattcga tttccgcccg cggctgaagg tcaatctcgt cgatatcggt
    10201 caatcgttcg ttccctttct gacagggcat ctcaatttta tcagcagcgc ggtgaacaat
    10261 cattggtatc tggtagtccg ggagccgggc acaaaccgga tgtatacgtg gccgacgggt
    10321 atctattcgc cggatatccc ggcgatcagc cgggcgatgg agaaggtgtt gctggatgga
    10381 tctggcgggg aaatccggag cgccgcaggt agccgcgggc agcagatgta tgaagccgtt
    10441 aggggctccg gtaattttcg cgagggaccg gttttgccgg aaatgcggct gccgcctttc
    10501 cagctaccct actacgaggg cttcaaccgt gcgggcaata aaagctggtt gggcatctac
    10561 cgccgggaag agttattccc gctggccttt ttgaaagatg tttgtttgct ggccttgcag
    10621 acgaagaacg ggcagctata tacgacgccc tggaaatcgc tgctgatcaa aaatatcggg
    10681 gacccggatc tgtcacgctg ggaattcgta ctgggcagat accgtatgaa tgtccggcat
    10741 gcttccaacg agctgaactg gcagatcgag gatctgtgcc cggacggttt gcggttgaag
    10801 cggtacctcg tccggcagat ggaccaggac gatgtccgga cggaggggct tagctttacg
    10861 atcaagacgc aaaagggttc ggggttggcg gggtcggtgg tcatccggct gcaggagggg
    10921 gccagatccg gtcagcacaa ggtgctggac cgttattcga tatggcatac ggaagagttc
    10981 gacgcacatg cccgggagtc ggtcctctat cgcaaggatc tcgaaaagga gacccttttt
    11041 ccctatttgg tatcgttgtg caaagccttc tacgagcagc aaagcgggga cccgatattg
    11101 gcggtggaac cgggaacggg tatcggcgcc atcgccaggc aggagcctgg ttttcagtgt
    11161 cgtcattgtc tgacactgta tgaagagcag tatggcgatc cggatcaggc tgttttgccc
    11221 gggacaccgt tcggtcggct gggggccgat tatgtctgtc ccgtttgcgg agcgccgaag
    11281 gcggatttcg acgcgagtgt cgtaacttta ggatgattat gatggctcct ataaaagacg
    11341 attatggaag aacgttccgc accttgcggg taagcctgtt gcagcattgc aatctgggtt
    11401 gtgtttattg tgtggcgggt gaggaccagc tgaaacaggc caatgcttcg cctgcaggcg
    11461 gatcgctggc ggtcggcgag ctgctggcca tcatccggcg tctgcatgga cagctggaac
    11521 tggatcatgt ccggctgacg ggcggggagc ccctgctata tccggagctg ccggcgctga
    11581 tccggggtat ccgggatgcc ggcattgcct cggtccggat gacgaccaat ggttttttgc
    11641 tggagcgaca ggctgcgtcg ctgaaagagg ccggtctcga agcattgaat gtctcgctgg
    11701 atgccgtcga tgaggaggtg ttctttcgga tcagcaggag gcggtctgcg gtaaggatca
    11761 tcaagggcgt cgatgccgcc ttgcaggcgg gtctggccgt caagctcaat gcggtcgtaa
    11821 tgaaagggat caacgatagc gaaatattgc ctttgctgga tctcgccatt tcgcgcggga
    11881 tcccgatccg tttcctggaa gtgatggcga tggggcatct gcatgccgat gcggatcgct
    11941 atctttttcc acaggaggag atcctgcggg tgatcggggc gcgttatcct tgcgagccat
    12001 tgcccagggc agcaggcgcc acggcgcggt attggaagac ggaaaacggg catgtcttcg
    12061 gcatcatcgc caatgagagc gagccgttct gccgcgattg cgatcggctc cggctggaca
    12121 gcagcgggaa gatctatggg tgtttgagca gcaaccaccc catcgggctg ggtgtcgatg
    12181 aaggcgacga cgaatggagg gacaagctgc agcgggcact gtcgcagaaa caggccctgc
    12241 gttttgtggg tagtgatctg agtatgttaa ggatcggcgg gtaagagaag ttcgaaaatg
    12301 aaaattcacg tatacgccgg gttgaaagat tactttcagc cggaattcga gatcgaaagt
    12361 ttcgcccgga atacggacga gctgaaggtg ttgttgctgg ccattaacga taaggcggag
    12421 aagatgctat cctcctgccg gtttgccgtg gaagaaggat ttatcgacaa tgaatttaaa
    12481 ttgaatgagc atgacaccat cgtcatcatc ccgccggcta gcgggggata ggggcgccgg
    12541 ggaggggggc gaacgagtag cggctggcag gcccgggggt gaggaggccg gacgggtcgg
    12601 cgcgctgagc gccggaccga tcgatatggc cggaatgctg gcacaggcgc atcatccggg
    12661 cgcaggcggc gtcgtattat tcagcggcga agtgcgggat agcaatcatg gccgggcggt
    12721 agggttcctg gaatacgagg cgtatgcgcc gctggcggaa aagatgatcg gggagatact
    12781 cgctactgcg aaagagcgct gggagcttca tattgcgctg gcgcaacacc ggacagggag
    12841 ggtggacgtc ggcgatacgg ccgtcgtggt gatcaccgcc tccgcgcacc ggagcgaggc
    12901 gtatgcggcc aaccgttata ttatcgaccg gatcaaacat gaggcgccga tctggaaatg
    12961 cgagcactat gccgacggcg gcaaggaatg gggcaataat tgtaattgtc atgccgtgac
    13021 cggcgatgtt tccaaacaca tttatgaaag cgaacgctga cgcgcccggg cggtcgaagg
    13081 atgggcgcat cgctgatctg cttggcgtga tcctctgcgg gggtgagagc cggaggatgg
    13141 gcagggataa gggattgttg cttgccgatg gggttccatg ggcgctgcat atggcgggaa
    13201 agctggggcc atggggtcta ccggtcgtct attccatcaa taaaggtcag tacgagactt
    13261 attcggcgca tttgcccggg cagaggctgg tagtggacag gctgggtctc gcggggccgt
    13321 tggaagggtt attgaccgtc catgccgcgt atcccggatc cgatctgttg ttgctggcct
    13381 gcgatcttca ggacctcgat gcggaaacgt tgtcgggcct gatcgggaag tatcttgcgg
    13441 aagaggccaa tgcctatgct tatcgggatg cggacggttt gcaaccgttg tgcgcgattt
    13501 atacatcggt gttgttgcgt ccgagatatg aggcggtgaa ggagggtcgt gcgatcgacg
    13561 tccggcttcg gtcgctgctg gatggtgagg gggtaaggtt gctggaggcg gggagcgaag
    13621 gggcgttcag gaattataat acgttataga aaaggacgtc cgcggatgca ggtggctagt
    13681 ttaactcaaa tgcgtgtttc tcccggttgt gtacgaccat cgcggccaga agaaccaatg
    13741 gttagtatgc agatacgaat gccggcgcca tttgacagga accctgggct tcccaggtga
    13801 aggatgattt gaggagcctc ttgaaattaa atattctggc gaatatcatt tttcattgcg
    13861 actttggcct tgctgccgcg atcctggcaa atttgattgc tcgttcttgg aaggatgatt
    13921 atctttaggt tgatatggga gagaaggcct ttgtaacccc ggctgtgttg aagtggtccc
    13981 gtgagactgc ccggatgact gtagagcagg ctgcggggaa ggccagcgtg agcgcgcaac
    14041 aagttgtcga ttgggaatcc ggccatgatc gtcctacgat ccgccaggcc agattgctgg
    14101 ccgaagccta ccgccggcca tttgcgttgt tcatgttgcc cgcgccaccc agggattgga
    14161 ccccattaaa agactttaga cgaaggagcg cgattccctt taccacagcg accgagtttt
    14221 tgatcaggga cctgcgtgtg aaacagactt gggctagcga atggaatcgc gagcaaggcg
    14281 aagcgccagt gccctttgtc ggacggttta cgctccggca tagtcctgca aaggtggccg
    14341 ccgatatctt gaaagagttg gatattcacc ctccgaacta tgccaacccg ctaaaagaat
    14401 ggctaactaa agcagaattg aaaggcatct atatttcaag gaccagttac ctgaactccc
    14461 acctgacgct ggatagtgaa gaatttcaag gtttcgcaat agccgatccg atggcgccac
    14521 tcattttcat caatacggat gattgggata ctgcgcagct atttacactg gtgcatgaac
    14581 tggcgcatgt ctggattgga gagaccggaa tctccaatga agtagccctg aattttaaat
    14641 cgctaaacaa tattgaccag gtggaacgct tctgtaacca ggttgcgggg atcgctttaa
    14701 tgccagatgg ttttgttagc gttctgcctg cctccacctt cacttccttg gaggaggtcc
    14761 ttgcgcaggc tcggcgctgc ggagtaagtg gttttgcgct gctggttcgg gcgcggaatc
//
LOCUS       DASYSV010000104         5200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1428836, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000104 DASYSV010000000
VERSION     DASYSV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428836"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..475
                     /locus_tag="VGQ51_03270"
     CDS             59..475
                     /locus_tag="VGQ51_03270"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV9035610.1"
                     /translation="MSNRTFTMIKPDAFSKGHSGAIIDKILKSGFRIVAMKLTRLSKE
                     TAGEFYAVHKARPFYDELVEFMSSGPIVAAILEKDNAVADFRKLIGATDPAKADEGTI
                     RKLYAASVGENAVHGSDSDENARIEGDFFFNAFERV"
     gene            complement(501..1499)
                     /locus_tag="VGQ51_03275"
     CDS             complement(501..1499)
                     /locus_tag="VGQ51_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEV9035611.1"
                     /translation="MGGAGAGPPDCFSIAKIQGSIIRRGGRSIMMRKIYLLFAGTLIA
                     LLGIAQTPHSLLWRISGKNIMRPSYLFGTMHVLCAADARLSDSLTAAIKNCDEIWFEI
                     DLSDMAGMLGSMQYMQMNDSKRLSDLLKPDDYARVKAYFAKHMAMMPFTMLERFKPML
                     ISALIEQQELDCETTDGMELRIMKESKTWHKPINGLETAEFQASLFDSIPYEEQAREL
                     VKSIDSSDQNKKMTQQLVRLYNQQDLDGIDSLSQQDDQEMSAYMDLLLYGRNRKWAKK
                     LDGMLRKKSMLIAVGAAHLPGKGGVIALLRKEGYTVEPVQNQLTNGQVADVARPAR"
     gene            complement(1409..3316)
                     /locus_tag="VGQ51_03280"
     CDS             complement(1409..3316)
                     /locus_tag="VGQ51_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEV9035612.1"
                     /translation="MAYRNQQDFIQALEKAGELVRISAFVDPKLEMAEITDRISKSGK
                     GGKALLFENNGTDFPVLMNAYGSERRMCMALGVSGLDDVTREIEGLFKLLSSPKEGLL
                     DKLKLLPKLSQFAGWMPVVKSGRGECQEVILPNPDISRLPVITCWPKDGGPFVTLPII
                     HTKDPTTGIRNVGMYRMQVFGPTLTGMHWHKHKVSARHFSEYKKTGRKMPVAVALGGD
                     PVYAYSATAPLPENVDEYMLAGFLRKKKVQLVSCITQPGIEVPADADFVIEGYVDPTE
                     ELIWEGPFGDHTGYYSLPDWYPRFHITAITHRKNAVYPATIVGIPPQEDAWLGKATER
                     IFLAPIKMTLVPEIVDMDMPVEGVFHNLVIAQIKKEYAGQGQKVMNAMWGAGQMMFNK
                     ILVLADETANIRDYAQLARKVFANLNPATDIYFSQGPMDVLDHSCSKLGFGGKMCIDG
                     TRKMEEEKDTDLHEETAGDDVTESLFMDRWPEITAVNLSLLQQQLPCLILSVRKDRPG
                     HIRELHNAIRALDAVEGTKLILYVEHTVDARDLPVALWRLCNNLDPRRDHILSERASN
                     KIACMGLDGTRKTRELDNFQRDWPNIIVADDTTIENVDRKWEELGLGPLIASPSLKFR
                     DQLYGGEAVVS"
     gene            complement(3452..4303)
                     /locus_tag="VGQ51_03285"
     CDS             complement(3452..4303)
                     /locus_tag="VGQ51_03285"
                     /inference="COORDINATES: protein motif:HMM:NF039345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HEV9035613.1"
                     /translation="MKRFLLAGILLPLFAHAQWNVNLYGGFSNYIGDLQSSPYTTKQS
                     NGSFGAGIQYDLSSHISLLSNLTYGRISAADGYSNQADLRARNLNFESVIKEWNVLAE
                     YNLLDLNEHRFTPYIFAGLAIFHFDPYAYDTTGRMVYLKPLSTEGEGLPQYASRKPYA
                     LTQLAIPFGGGIKFRINDRVVLAYEIGMRKTFTDYLDDVSTRYVDQGTLLAAKGPEAV
                     EMAWRGNEIKNGGPYPPDGTIRGGAKRKDWYYMSGIRVTIALNNPYNPSSHRRHSAIM
                     DCPKKVY"
     gene            4591..4664
                     /locus_tag="VGQ51_03290"
     tRNA            4591..4664
                     /locus_tag="VGQ51_03290"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4625..4627,aa:Pro,seq:ggg)
     gene            4950..>5200
                     /locus_tag="VGQ51_03295"
     CDS             4950..>5200
                     /locus_tag="VGQ51_03295"
                     /inference="COORDINATES: protein motif:HMM:NF036423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEV9035614.1"
                     /translation="MRTSHSFSIEFLKRGCRGRKKKGKAHIYARIWVDGEAAEISLKE
                     EINEKDWDPQAEQVKGRNIQVKSLNDHIDCVRDKIRNKYR"
BASE COUNT         1182 a         1394 c         1356 g         1268 t
ORIGIN      
        1 acattaccta gctttgcccc ccaaaaaaga tactttcacc agtcaaaaag aaaggaatat
       61 gagcaatcgc acattcacaa tgatcaaacc ggacgccttt tccaaaggac actccggcgc
      121 catcatcgat aagatcctta aaagcggctt tcgcatcgta gccatgaaac tgacccgtct
      181 gtccaaagag acggccggtg aattctatgc cgtccataaa gcccggcctt tttacgacga
      241 gctggtcgaa ttcatgagca gcggccccat cgtggcggcc atcctggaga aagacaatgc
      301 cgtagccgat ttccgcaagc tgatcggcgc aaccgacccc gccaaggctg acgagggcac
      361 gatccgcaag ctgtatgcgg catcggtagg agaaaatgcc gttcatggca gcgacagcga
      421 tgaaaatgcc cggatcgaag gggatttctt cttcaatgcg tttgaaaggg tgtaactcat
      481 gacgccggct ataccggaag ctagcgtgcg ggacgggcaa catcagccac ttgtccgttc
      541 gtcagctggt tctgtacggg ctcgaccgta tatccctctt ttcgcagcag ggcgatcaca
      601 ccgcctttgc ccggcagatg cgcggcgcct acggcgatca gcatcgattt tttccgcagc
      661 attccgtcca gcttcttcgc ccatttgcgg ttgcggccat agagcaacag gtccatgtag
      721 gcactcatct cctgatcgtc ctgctggctc aacgaatcga tgccgtccag gtcctgctgg
      781 ttgtagagac gcaccagctg ttgggtcatt tttttgttct ggtcactgct gtcgatggat
      841 ttaaccagct ccctggcctg ctcttcatat ggaatgctgt cgaaaagact cgcctggaac
      901 tccgcggtct ccagcccgtt gatgggtttg tgccaggtct tcgactcctt catgatcctc
      961 agctccattc cgtccgtggt ctcacaatcc agctcctgtt gttcgatgag cgcgctgatc
     1021 agcatcggtt tgaaacgttc gagcatggtg aacggcatca tagccatatg tttcgcgaaa
     1081 tatgctttta cccgggcata gtcatcgggt ttcagcagat cggacaaccg cttgctgtca
     1141 ttcatttgca tgtattgcat cgaacccaac atgccggcca tgtcgctgag gtcgatctcg
     1201 aaccagatct cgtcgcaatt ctttatcgcg gctgtgaggc tgtcgctcag ccgggcgtcc
     1261 gcggcgcaaa ggacatgcat cgtgccgaag agataggaag gacgcataat gttcttcccc
     1321 gaaatgcgcc agagcaggga gtgaggcgtc tgggcaatac ccagtaaagc gatcagtgtt
     1381 cctgcaaaca gtaagtaaat tttccgcatc atgatactac ggcctccccg ccgtataatt
     1441 gatccctgaa ttttagcgat ggagaagcaa tcagggggcc cagccccagc tcctcccatt
     1501 tcctgtcgac attttcgatg gtcgtgtcgt ccgcgacgat gatattcggc cagtcccgtt
     1561 gaaaattgtc cagctcgcgt gtcttgcggg tgccgtccag tcccatacat gcgatcttat
     1621 tggatgcccg ttcgctcaga atatgatccc gccgcggatc aaggttattg cacaaccgcc
     1681 ataacgccac cggcagatcc ctggcatcta ccgtatgctc gacataaagg atcaatttag
     1741 tgccctccac ggcatccagc gcgcggatgg cattgtgaag ctcgcggata tggcccggcc
     1801 tgtcttttcg caccgaaagg atcagacagg gtagttgctg ttgaagcagc gacagattga
     1861 cggctgtgat ctccggccag cgatccatga atagcgactc ggtgacatca tcgccggctg
     1921 tctcctcatg gagatccgta tccttttctt cctccatttt gcgtgtacca tcgatacaca
     1981 tcttcccccc aaatcccagc ttgctgcaac tgtgatccag cacatccatc gggccctgcg
     2041 agaaatagat atccgtcgcg ggattcagat tggcgaatac ctttctcgca agctgcgcat
     2101 aatcgcgaat gtttgccgtc tcatccgcaa gcaccagtat cttgttgaac atcatctggc
     2161 cagcgcccca catggcattc atcacttttt gaccctgacc ggcatattct ttttttatct
     2221 gcgctatgac gaggttgtga aaaacgcctt ccacgggcat atccatatct acgatctccg
     2281 gtacgagcgt catctttatc ggagcaagga agatgcgttc ggtcgctttt cccagccagg
     2341 cgtcttcctg tggcgggata ccgacgatgg tggccggata gacggcattc ttacgatggg
     2401 tgatggcggt gatgtgaaaa cgcggatacc agtccggcag agaatagtag ccggtgtggt
     2461 cgccgaaggg cccttcccag atgagctctt ctgtcggatc gacatacccc tcgatgacaa
     2521 aatcggcgtc tgccgggact tcgatacctg gctgagtaat acaggatacc agctggacct
     2581 ttttcttgcg aaggaaacca gccagcatgt attcatcgac attttccggt aagggagcgg
     2641 tagcgctgta agcgtaaacg ggatcgccgc cgagcgctac ggctacgggc atcttccttc
     2701 ccgtcttttt atattcggag aagtgccggg cactgacctt gtgtttatgc cagtgcatgc
     2761 cggtcagcgt cgggccgaag acctgcatgc gatacatccc gacattacgg atgccggtcg
     2821 ttgggtcttt ggtgtgaatg atgggaagag tgacgaaggg tccgccgtct ttcggccagc
     2881 aggtgatcac gggcagcctg gaaatatccg ggttggggag gatgacctcc tggcattcgc
     2941 cgcgtccgct cttaacgacg ggcatccatc cggcgaactg gctcagtttg ggcagcagtt
     3001 tcagcttatc cagtaaacct tctttggggg aggaaagtaa tttgaacaac ccttcgatct
     3061 cgcgggtgac atcgtcgaga cccgaaacgc ccagcgccat gcacatgcgt cgctcgctgc
     3121 cataggcgtt catcaatacc gggaaatccg tgccgttgtt ctcgaacaac agggctttgc
     3181 cgcccttacc gctcttactg atcctgtcgg tgatctccgc catctccagt ttcgggtcga
     3241 caaacgcgct gatccgaacc aattcccctg ctttctccaa tgcctgtatg aaatcctgtt
     3301 gattcctgta cgccatgacg taaatatact atgtgccggg gattcgctgt ctgaaaatta
     3361 gcgaatcccg gccaagatat tatgtctgtc ctatcggaca aagcctcctc cgtcggcttt
     3421 tggtcggctt tggcggtggc ccgttctcct ctcaataaac ttttttagga caatccatga
     3481 tggcgctatg tcgcctgtgc gaagacggat tatagggatt attaagggcg atggtcaccc
     3541 gtatcccgga catatagtac cagtccttgc gcttggcgcc tccgcggatg gttccgtcgg
     3601 gaggatacgg cccgccgttc tttatctcat tgccgcgcca ggccatttcg acggcttccg
     3661 gcccctttgc cgccagcagc gtgccctgat cgacatatct ggtgctgaca tcgtcgagat
     3721 aatcggtgaa tgtctttcgc atacctatct cataggcaag caccacccgg tcgttgatcc
     3781 tgaacttgat acccccgccg aaggggatcg ccagttgggt gagtgcatag ggtttccggc
     3841 tggcatactg aggtaggccc tccccttccg tactcaatgg cttcagatag accatgcgac
     3901 cggtggtatc atacgcatag ggatcgaaat ggaagatcgc caggcccgcg aagatatagg
     3961 gcgtaaaccg gtgttcattc agatcgagca ggttgtattc ggcaagcaca ttccattcct
     4021 tgatcaccga ttcgaaattc agattgcgcg cccgcagatc ggcctggttg ctgtagccgt
     4081 ccgccgccga gatgcgtccg taagtaagat tggaaagcag cgaaatatga ctgctgagat
     4141 catattgtat cccggctccg aaggagccgt tggattgctt tgtcgtgtag gggctggatt
     4201 gcagatcgcc gatgtagttg gaaaaacctc cgtataagtt cacattccat tgggcatggg
     4261 caaacagtgg caatagtatc cccgctaaga ggaatctttt cattgaatgc agaatttgtt
     4321 aggcaaagtg gttaggctaa cgccaggctt tcgctcgcgt aaagtttctc gcagggatgt
     4381 ggatcggttg ctacgtttgg gggaggtttt accgcataga actatacggt tgaagagtta
     4441 ttggagttgc tgctgtaagg caactattaa gccaattatt tgaaaaagga gacatcaaaa
     4501 aaaagttaag ggtttttcgg cggcaaaacc gcttttccct acctttgcac cccacgaaac
     4561 ctcctgatcc tcatcgggca gggcggtttt cgggacgtag cgcagcccgg tagcgcactt
     4621 gcatggggtg caaggggtcg ctggttcgaa tccagtcgtc ccgacaataa gacagatcaa
     4681 aatcgatcaa agcctgtaag tattcactat ttacaggctt ttttcatgcc cagggcatca
     4741 aaaagtagca taaaaaggca aatttctggt gaacaaatcc ggtacaagat ttgtgttcac
     4801 tactttcccc gcaattcatt gcctgacggt cgattagctc aattcttttg agcttttttg
     4861 atacgattcg acaactggat tcccaccgcc taacactgac aattccgaag agcaaggacg
     4921 aatcatttat caaagtcaaa aaaacgggta tgagaacgtc gcacagtttc agcattgaat
     4981 tccttaaacg cggctgccgt ggtaggaaga aaaaagggaa agcgcatatc tatgcgcgca
     5041 tttgggtcga tggcgaggct gcggaaattt cgctgaagga agagatcaac gaaaaggatt
     5101 gggatccgca ggccgagcag gtaaagggcc gcaacattca ggtaaagtct ttgaacgatc
     5161 atatcgattg cgtccgcgac aaaatccgca acaaataccg
//
LOCUS       DASYSV010000105        10339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2196755, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000105 DASYSV010000000
VERSION     DASYSV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10339
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196755"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VGQ51_03300"
     CDS             complement(<1..604)
                     /locus_tag="VGQ51_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HEV9035615.1"
                     /translation="MRRSIRILIASLLALFLAGQTARAQMGEVRFGPPGNDSVKVIHI
                     LSDDKYHFNQIDSVNSSTTLVGNVGIRQEKTTIYCDSLIMYPHLNYIECFGKVHINDN
                     DSVNIYSDYMKYMVDTKLVYFQKNVKLTDGKGVLTTEDLNYDLNQHVGTYTNGGRIVN
                     KESVLTSQRGVYYEDTKDIHFYDNVVLRDPQYDLSADSLLY"
     gene            complement(601..1599)
                     /locus_tag="VGQ51_03305"
     CDS             complement(601..1599)
                     /locus_tag="VGQ51_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV9035616.1"
                     /translation="MTKISNETKIGVLAVVALAALILGFNFLKGSSLFKNTKHLYAVF
                     DNVEGLEVSNAVQIDGLTIGSISAINESDKDLSNGIVVTITLKKDVHIPKNSIGEINS
                     GFIAAATIVIRRGDETQYLVNGDTLRTMQKANLFAQVQSSVNPVILKLGGTLTSLDSL
                     IEVVGTMFDPRLKNNFSAIIANMAVTSAELQRMLNTQTGAIAQSLKNMNEFTANLAKN
                     NDHITHTLDNLEKASDSLAASKIPQTVESLRTTMGDLRAAIDKVNSPNGTLGLLINDK
                     GLYQNMVNTTRSLNILLDDLRAHPKRYVNISVFGKKDKNGYLTAPLDSTASKPGNK"
     gene            complement(1738..2694)
                     /locus_tag="VGQ51_03310"
     CDS             complement(1738..2694)
                     /locus_tag="VGQ51_03310"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV9035617.1"
                     /translation="MTLLGALVCALTSFQAGGPRPPQRQALRTIVIDPGHGGFDTGTR
                     GLFSSEKDVALSISLKLGQMIAETWPDTKIVFTRTTDVMPGGGSTIASGLNYRADLAN
                     RSRGDLFIAVHCDNDGHPAGPFEVHRIIGHKWVGKKRRHRVPIYETYEGHHTRKGTET
                     FIWKADRSGFKGSAINERGAGELSDSSGETNSDSAAFDMNSPEAMVRARLYEQKYFGN
                     SALFATLVEDEFSKAGRASEGVFQRDEGIRVLQATGMPSVLIETGFLSNVDEEKYLNS
                     EEGQEEVARNILDALKRYKATLEGHPISVTELPSGTRGTSSR"
     gene            complement(2797..3630)
                     /locus_tag="VGQ51_03315"
     CDS             complement(2797..3630)
                     /locus_tag="VGQ51_03315"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV9035618.1"
                     /translation="MISPILRTIVIDPGHGGFDPGTHGLLVKEKDVALGISLKLGKAL
                     QQAFPGIRIVYTRTTDIMPGNKSTIQEGIRYRAELANRSNGDLFICIHCNSNGKAAGS
                     YPVRHLVRYRIIGKGRHKRSVPVYATAWVKNMTHGTTTYIWKADRNEKKGAAVDAELR
                     NGENMGDSTGTAFDFSTPEAMMRAQLYEKKYFANSALFGTLVEDEFARTGRRSYGVQQ
                     RPVGIGVLEATGMPSVLIETGYLTNRDEERYLASNKGQNEVARDIVNALKRYRSLAAN
                     Q"
     gene            4106..6625
                     /locus_tag="VGQ51_03320"
     CDS             4106..6625
                     /locus_tag="VGQ51_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HEV9035619.1"
                     /translation="MLASKRVDTLDYKVSKDTLEAPISYKASDSIVMIIPTRSIILYN
                     KADAKYKDAELMADHIVYDQDRNVVVAKPGVDSAGNPMGLPKIVQTDNTMTSDSIIYN
                     IKTGKAFTQGTVTNSGEMFVHAEKMKKVTPDVYYGWRGVFTTCDLDTPHFAFRTNKMK
                     MINKKFAITGPIHPEFEGVPLPIYLPFGFFPLTQGRHSGLLPPTFSASDQAGLGLEGL
                     GYYKVLSDNFDVTVRTNLYSYGGWNVNILPEYKVRYHYAGNMSFSLQNTRLLADAGKD
                     AYTTQRTFNFNWTHQMDQKARPGTSFGANVNVASSKYNQYLLNNPTANYQNQLSSSIT
                     YSKTWDGKYNLTLSANHNQNNESRLVNVSLPNLTFTAPTIYPFTSTTFIGTPKWYQKL
                     GVGLSTTITGAASFYDSLFSLQRIVDTFQWGAQNSIPISLALPIKGPIQVSPGISLQN
                     RVYSRKLYRVYDPITNKVDTTGIEKGIYTANNMSFSLSLSTAIFGTFQNFGKHSSLLG
                     IRHVIRPTMSFSYSPDLSGAYYYNLTTPYDTAAGVPKITHNQRVSYFDGSTYGPFGEG
                     RFGGISFGLDNHIEIKTRSKSDTSESGVKKMTIIDGFGFNGSYNYLADSFKLSPITLY
                     FRSTLFKNINITGGATLNPYQTDSFGFNINKYAWEARKFSLGRITTGNLAVSTSFKSK
                     PKDDKLAQQKEKEQQDNQIPMTMEEQQAQLNYIRNNPAQFADFNIPWSLNLSLAFNFQ
                     NAEKPDYSGFATTITSSLNWSGDFNLTEKWKVMLSGYYDLKATQMQSLTMSISRDLHC
                     WQMSINVTPVGYSHYFSITISPKSGILQDLKINRTKYFINE"
     gene            complement(6672..7436)
                     /locus_tag="VGQ51_03325"
     CDS             complement(6672..7436)
                     /locus_tag="VGQ51_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV9035620.1"
                     /translation="MDSRNKSVFWSVFGRLWAVWVIVIFVLTMLIFLIPFLLFSYFRP
                     DPQKTRRWASIARAWMAVFLPLAGCPLRVRGREKFVKGQSYIVTCNHNALIDVPVSYP
                     GIPGGNKTIAKIEMAKIPIFGMFYRTGSVLVDRSSEASRKDSFTKMKEVLDMGLHMCI
                     YPEGTRNKTADPLKSFHDGAFRLSLTTGKAIIPAIIFNSRKIMPPDRTFFLRPCPLYI
                     HFLDPVMPAPDETVESLRQRIFTVMWNYYMAGPRQK"
     gene            complement(7495..8775)
                     /locus_tag="VGQ51_03330"
     CDS             complement(7495..8775)
                     /locus_tag="VGQ51_03330"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9035621.1"
                     /translation="MNRRPAHRNRTSTTTHRYGIITTGSRGDIQPFVALALTLIRRGR
                     DVTIVASENFKDFVEGFGIAFRPITGDSERIINSPEALKLLKGGNVFKFVYHLLKISE
                     RSAHQSNRDILNACEHFDHLIVSVLPLPYVYSIAERYRKKCAVIFLSLPPIPTREFPY
                     QALNLPIPSWLNRLSYRMVGLAYRMTSKQIDRFRMGIGLPPGNVLKAILGSDILAILA
                     ESRHLVRQPADWPPNAHVSGFFYLPPSAAASPTPLLEDWLHAGTQPVYIGFGSIPIPD
                     PTRFYNVLQGVLAKNRVVLATGWSVLDDLPRHPGLFTTKYIDHEWLLPRCSAAVIHGG
                     IGTAAAVLKSGIPIIVVSILADQPINGKMIRKRRLGFHIPFKKLTTEKLLQALEDAQA
                     EEIKTNCRFIAEQIRLENGVEKAARLIEDYFEDA"
     gene            complement(8782..9459)
                     /gene="ung"
                     /locus_tag="VGQ51_03335"
     CDS             complement(8782..9459)
                     /gene="ung"
                     /locus_tag="VGQ51_03335"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV9035622.1"
                     /translation="MDVKIEDSWKEALRQEFTKPYFLQIVTLLKNEKISGKTIYPPGS
                     LIFNAFNTTPFDKLKVVILGQDPYHGPGQAHGLSFSVPKGIPPPPSLINIFKELNQDV
                     GMAIPSHGNLTHWAEQGVLLLNASLTVRANEPMSHSKIGWAEFTDAVISKVSALKEHT
                     VFLLWGKFAQEKQSLVDETKHLVLKAAHPSPFSADKGFFGCRHFSKANAWLAGNRIDP
                     IDWSLGN"
     gene            9605..9678
                     /locus_tag="VGQ51_03340"
     tRNA            9605..9678
                     /locus_tag="VGQ51_03340"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9639..9641,aa:Arg,seq:tct)
     gene            10073..>10339
                     /locus_tag="VGQ51_03345"
     CDS             10073..>10339
                     /locus_tag="VGQ51_03345"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEV9035623.1"
                     /translation="MPNTPEYIPQLVGEIYRARSRSVFATLIRLLKDFDLAEDAMHDA
                     FKAALEQWGEKGIPSNPLAWLVSAGRFKAIDRIRRESKFDSLEEQ"
BASE COUNT         2277 a         2581 c         2953 g         2528 t
ORIGIN      
        1 tgtacagcag tgagtctgcg gaaagatcgt attgaggatc gcgaagaacg acattgtcat
       61 agaaatggat gtctttcgtg tcctcgtaat agacaccgcg ctggctggta aggacggatt
      121 ccttattgac gatgcggccg ccattggtat aggtgcccac atgctggttg agatcgtagt
      181 tgaggtcttc ggtagtgagg acgcctttac cgtccgtgag tttgacattt ttctggaaat
      241 agaccagttt tgtgtccacc atgtatttca tgtaatccga gtagatattc accgaatcgt
      301 tgtcgttgat gtgcactttt ccgaaacatt cgatgtaatt cagatgcggg tacatgatga
      361 ggctgtcgca atagatggtg gtcttttcct ggcggatgcc gacattgcct acgagggtgg
      421 tgctcgagtt gaccgaatcg atctggttga aatggtattt gtcatcgctg aggatgtgga
      481 tgaccttcac gctatcgtta cccggggggc cgaatcttac ctctcccatt tgtgcccggg
      541 cggtttggcc tgccagaaaa agggccagca ggctggctat taaaatacgg atgcttctcc
      601 tcatttatta ccgggtttgg acgcagttga gtccaggggg gcagtcaggt agccgttctt
      661 gtcctttttc ccgaatacgg aaatattcac atagcgtttg ggatgggccc gcagatcgtc
      721 gagaaggata ttgagactgc gggtggtatt gaccatattc tgatagagac ctttgtcgtt
      781 gatgagcagt cccagcgtgc cgttggggct gttgacctta tcgatggcgg cgcgaagatc
      841 gcccatggtg gtccgcaggc tttccacggt ttgtgggatt ttggaagcgg ccaggctgtc
      901 gcttgccttt tcgagattat cgagggtatg ggtgatatgg tcgttgtttt tggccagatt
      961 ggccgtgaat tcattcatat tcttcagcga ctgcgcgatg gcgcccgtct gggtgttgag
     1021 cattcgctgg agctccgcgg aggtcaccgc catattggcg atgatggcgc tgaaattgtt
     1081 cttcagccgt gggtcgaaca tcgtccctac gacctcgatc agcgagtcca gcgaggtcag
     1141 ggtaccgccc agtttgagga tgacggggtt gacgctggat tgtacctggg cgaaaagatt
     1201 ggctttttgc atcgtccgca gggtatcgcc gttgacaagg tattgggttt cgtccccgcg
     1261 ccggatgacg atggtcgctg ccgcgataaa acccgaattg atctccccga tggaattctt
     1321 agggatgtgg acgtcttttt tcagcgtgat ggtaacgacg ataccattgg agaggtcttt
     1381 gtcggattca ttgatggcgc tgatgctgcc gatggtcaga ccgtcgatct gtacggcgtt
     1441 ggagacctcc agcccttcta cgttgtcgaa gacggcatag aggtgtttgg tattcttgaa
     1501 caggctgctg cctttgagaa aattaaaacc gaggatcagg gcggccaggg caacgacggc
     1561 caacacgccg atctttgttt cattggaaat tttggtcatg cattaaggtt tagccattca
     1621 aacgggcctc taatctacaa atgtaggaaa attgccgtca ttacggcaga agacaacgtt
     1681 aaactgtggc gggtcgatcg attgtcgaca cgtgtgatgt gggcggtggg ccgatcgtta
     1741 ccggctggat gttccgcggg tgccggaggg gagttcggtg acgcttatcg gatggccctc
     1801 gagggtagcc ttataccgtt ttaatgcatc gaggatattg cgggccacct cttcctgacc
     1861 ttcttcgcta ttgaggtatt tttcttcgtc tacattcgac aggaagcccg tttcgatgag
     1921 cacgctgggc atgcccgtgg cctgcagcac gcggatacct tcgtcgcgtt gaaagacgcc
     1981 ttcgctggcg cgtccggctt tggagaattc atcttctacc agggtcgcga agagggcgct
     2041 attgccgaaa tatttttgtt cgtagagtct cgcgcgcacc atggcttcgg gggagttcat
     2101 gtcgaaggct gcgctgtcgg agttggtctc acccgagctg tcggagagtt cgcccgcgcc
     2161 ccgctcgttg atcgcgctgc ctttgaagcc gctgcggtcc gccttccaga tgaaggtttc
     2221 ggtgcctttt ctcgtatgat gtccctcgta ggtctcgtag atgggtaccc ggtgtctacg
     2281 tttcttgccg acccatttgt gtccgatgat ccggtgaact tcgaaaggtc cggcgggatg
     2341 accgtcgttg tcgcaatgca cggcgatgaa aaggtcgccc ctgctgcggt tggccaggtc
     2401 ggccctgtag ttgagaccgg aggcgatggt agagcctccg ccgggcatca cgtctgtcgt
     2461 tcgtgtaaag actattttgg tgtcgggcca ggtttccgcg atcatctgcc ccagcttgag
     2521 cgagatggag agcgctacgt ctttttcgga ggagaagaga cccctggtgc cggtgtcgaa
     2581 tccgccgtgg ccgggatcga tgacaatggt acggagagcc tgccgctggg gcggccgggg
     2641 gccgcctgcc tgaaaggagg tcagggcgca gacaagtgcg cccaacaagg tcattgtaga
     2701 tagtttaaac atggtcattc actctggatc ttcttctgca gaatgggcca caggcccatt
     2761 ccaaaattga ataatggtat cgcgaccgcg gctactttat tggttagccg caagggagcg
     2821 ataacgtttt aatgcgttta cgatatcccg ggcgacttca ttctggccct tattgctggc
     2881 aaggtacctt tcttcgtcgc gattggtcaa ataaccggtt tctatgagca cgctgggcat
     2941 ccccgtcgcc tccagcaccc cgatacccac gggacgttgc tgaacaccat agctccggcg
     3001 gcccgtccgg gcgaactcat cttcgacaag ggtaccgaat agggcgctgt tcgcaaaata
     3061 ttttttctca tacagctgtg cccgcatcat cgcttcgggg gtgctgaagt caaaggcggt
     3121 gcccgtgcta tcacccatat tctcgccgtt gcggagctct gcatccaccg ccgcaccctt
     3181 tttttcattg cgatcggcct tccagatata agtggtggtc ccgtgcgtca tattttttac
     3241 ccaggcggta gcatacacag gaacacttcg tttatgccgg ccttttccta tgatccggta
     3301 tcggacaagg tgtcggaccg ggtagctgcc cgctgctttg ccattggaat tgcaatgaat
     3361 gcaaataaac agatcgccgt tcgaccggtt ggccagctcg gcccggtagc gaatgccttc
     3421 ctggatagtg gatttgttac ccggcatgat atccgtcgtg cgcgtataaa cgatgcggat
     3481 gccggggaaa gcttgctgca gggcctttcc caatttcagc gaaatgccca gcgcgacgtc
     3541 tttttctttc accagcagcc catgtgtgcc cggatcgaat cccccgtgac cgggatcgat
     3601 cacgatggtg cgaagaatcg gcgaaatcat tagcggtcgg ggagtcggca atttgttaaa
     3661 agacgtgaag ccaaacgctg ttccacaaag aagtaagaga gtggtcgtct ttttgatcat
     3721 agctatgtta ttttcccaaa taggttcaat cttcacataa tcttaatgga gttatggcta
     3781 tttttgacac gacagaacgg gccggaggtc cgttggccga aggccagaca ttagcgcttt
     3841 gcaggatatg cgctaatttt cggaaagtgc atatcctgta ctatttatga ataacggacg
     3901 caaaattagc tcaaaatatt tactggtgct gctttttact gctttattat tttcagtaac
     3961 gttcgacagc ccagcaaatt atctagctag cacccgtttt tgcaaaatca taacaactcg
     4021 cgacaccacc ctgccacgcg ccgtcctgcc gccactggca tcggtcaccg gagcgaggga
     4081 taccattctt cgagaagccg actcgatgct ggccagcaaa cgggtcgata ccctcgatta
     4141 caaagtgtcg aaagacacgc tggaagctcc catcagctat aaggcctcgg attcgatcgt
     4201 catgataatc cccacccgca gcattatcct ctataataag gcagacgcca aatacaagga
     4261 cgcggagctg atggccgatc atatcgtata tgaccaggac aggaacgtgg tcgtggcaaa
     4321 acccggcgtg gattcggccg gcaacccgat ggggcttccc aagatcgttc agacggacaa
     4381 cacgatgacc tcggattcca tcatttacaa tatcaaaacg gggaaggcct ttacgcaggg
     4441 taccgtcacc aactccgggg agatgttcgt gcatgccgaa aagatgaaga aggtaacacc
     4501 ggatgtctat tacggctgga ggggggtctt cacgacctgc gacctggata cgccgcattt
     4561 tgccttccgg acaaacaaga tgaagatgat caacaagaaa ttcgcgatca cgggaccgat
     4621 ccatccggag ttcgaaggag tgcctcttcc catctatcta ccgttcggct ttttcccgct
     4681 gacgcagggc cgtcattccg gcttgctccc gccgactttt tctgcgagtg accaggcagg
     4741 tctgggtctt gaaggactgg gttattataa ggtgctgagc gataatttcg atgtgaccgt
     4801 gcgtacgaac ctgtattcct atggggggtg gaacgtgaat atcctgccgg agtataaagt
     4861 tcgttaccat tatgccggaa atatgagctt ctcgctgcag aatacccggc tgctggccga
     4921 tgcgggcaag gatgcctata cgactcaacg gaccttcaat ttcaactgga cccaccagat
     4981 ggatcagaag gcccggccgg ggaccagttt cggcgccaat gtcaatgtcg cctcctcgaa
     5041 gtataatcaa tacctgctga acaaccccac cgccaattat cagaatcagc tgagctcttc
     5101 gatcacctat tcgaagacct gggacggcaa atacaacctg acgctgagcg cgaaccacaa
     5161 ccagaacaac gaaagccggc ttgtcaatgt cagcctgccc aatctaacct tcaccgcgcc
     5221 tactatttat ccgtttacga gcaccacctt tatcggcacg ccgaaatggt accagaagct
     5281 gggggtggga ctcagcacta cgatcaccgg tgcagccagt ttttacgatt cgttgttcag
     5341 cctgcagcgt atcgtcgata ccttccagtg gggcgcgcag aacagcatcc ccatcagcct
     5401 cgcgttgccg ataaaaggac ccatccaggt ctcgccgggg atcagcctgc agaaccgggt
     5461 ttattcgcgg aaactatacc gcgtctatga ccctatcacc aacaaagtgg acacgacggg
     5521 tatcgaaaag ggcatttaca ctgccaacaa catgtcgttc agtcttagcc tgagcacggc
     5581 catcttcggt acattccaga atttcggcaa gcacagctcg ctgctgggca tacggcatgt
     5641 catccgacct accatgagct tcagttattc tcccgatctt tcgggagcct attactataa
     5701 cctgacgaca ccatacgata cggcggcggg ggtgccgaag atcacgcata accagcgtgt
     5761 ctcgtatttc gacgggagca cgtacggacc cttcggtgag gggcgtttcg gcggcatttc
     5821 gttcggactg gataaccaca tcgagatcaa gacccggtcg aaatcggata cttcggaatc
     5881 gggtgtcaaa aagatgacca tcatcgatgg ttttggtttc aacggcagct ataactacct
     5941 ggctgattcc ttcaagctgt ccccgataac gctttatttc cggagcaccc tattcaaaaa
     6001 tatcaacatc accggcgggg cgacgctcaa tccctatcag accgacagct tcggtttcaa
     6061 catcaacaaa tatgcctggg aggcccggaa gttctcgctg gggcggatca ctacaggcaa
     6121 cctcgccgtc tccacctcct tcaagagcaa gccgaaggac gacaaactgg ctcagcaaaa
     6181 agagaaggag cagcaggata accagatacc catgaccatg gaggaacagc aggcccaatt
     6241 gaattatatc cgaaacaatc ccgcgcaatt tgccgatttc aatattccat ggtcgttgaa
     6301 cctttcgctg gccttcaatt ttcagaatgc cgagaagccc gactattcgg gtttcgcgac
     6361 gactatcacg tcgagcctca actggagcgg cgatttcaac ctgacggaga aatggaaggt
     6421 gatgctcagc gggtattatg acctcaaggc tacgcagatg cagtcgctga cgatgagcat
     6481 ctcgcgcgat ctgcattgct ggcagatgtc catcaacgtg acaccggtgg ggtatagcca
     6541 ttatttcagc atcacgatca gtccgaagtc ggggatcctg caggatctga agatcaatag
     6601 gacgaagtat tttattaatg aatagggggg cgcgggcttc gggcgccacc ggatgccggc
     6661 gtatccgatg attacttttg ccgcgggccg gccatataat agttccacat gacggtgaag
     6721 atccgttgtc tgagactttc gacggtctcg tcgggtgcgg gcatcaccgg gtcgagaaaa
     6781 tggatataga gcgggcaggg ccggaggaag aaggtccggt ccgggggcat gatcttccgg
     6841 ctattgaaga tgatggcggg gatgatggcc ttcccggtgg taagtgacag acggaaggcg
     6901 ccgtcgtgaa aagacttcag tggatcggcc gttttgttcc gggtgccttc gggatagatg
     6961 cacatatgga gtcccatgtc gaggacttct ttcatttttg taaaactgtc tttgcggctg
     7021 gcttcactgc tgcggtcgac cagcacgctg ccggtgcggt agaacatacc gaagatgggt
     7081 attttcgcca tttcgatctt ggcgatggtt ttgttgccgc cgggtatgcc gggataggag
     7141 acgggtacgt cgataagcgc gttgtgattg caggtgacga tataggactg gcctttcacg
     7201 aacttttcac ggccgcggac gcgcagggga catccggcca gcggtagaaa gacggccatc
     7261 catgcccgtg cgatcgaggc ccagcgcctg gtcttttgcg gatcgggccg aaaatagctg
     7321 aaaaggagaa agggaatgag gaagatcagc atcgtcagga cgaagatgac gatcacccag
     7381 acggcccaaa ggcggccgaa gacggaccag aaaacggact tgttacgcga atccatcctg
     7441 caatttaatt gaacgggcgc tatttggcgc atccggaccg acgattgggt gtaatcaggc
     7501 gtcttcgaaa taatcttcta tgaggcgggc tgctttttcg acaccattct ccagccgtat
     7561 ctgttcggcg atgaaacggc agttggtttt gatctcctct gcttgcgcgt cctctaaggc
     7621 ctgcagcagc ttttcggtgg tcaatttttt aaacgggata tggaaaccga gtcgccgctt
     7681 tcggatcatt ttgccattga tgggttgatc ggccaggatg gatacgacaa tgatcgggat
     7741 accacttttc aaaacggcgg cagcggtacc gatgcctcca tggatgacgg cggcggagca
     7801 gcgcggcagc aaccactcgt gatcgatgta tttagtcgta aaaagaccgg gatgccgggg
     7861 taggtcgtcc aggactgacc atcccgtggc cagcaccacc cggttttttg ccaggacgcc
     7921 ctgcaggacg ttgtaaaatc gcgtgggatc ggggattggg atactgccga atccaatata
     7981 gaccggttgg gttcctgcgt ggagccagtc ttccagtagc ggcgtgggtg atgcggccgc
     8041 ggatggcgga agataaaaga acccggagac gtgtgcattg ggcggccagt cggcgggctg
     8101 ccgtacgagg tgccggcttt cggcaaggat agccagtatg tccgaaccga ggatcgcttt
     8161 caatacattg cccggtggaa ggccgatccc cattcggaac ctgtcgatct gcttacttgt
     8221 cattcgatag gccagtccca ccatccggta agacaggcgg ttcagccacg acgggatggg
     8281 aaggttcagc gcctggtaag ggaattcgcg ggtaggtatg ggcgggagac taagaaagat
     8341 gacggcacat tttttccggt atcgctcggc tatcgaatag acgtagggca gcggcaggac
     8401 gctgacgatc aggtgatcga agtgttcgca ggcgttcagg atatcgcggt tggactggtg
     8461 cgcggatcgc tccgatattt tgagcagatg gtagacgaat ttgaatacat tcccgccctt
     8521 caggagtttg agggcctcgg gtgaattgat gatccgctcg ctgtcgccgg tgatgggtcg
     8581 aaaggctatg ccgaaacctt ctacgaaatc tttaaaattt tcggaggcga cgatggtgac
     8641 gtcgcggcct ctgcgtatga gcgtcaacgc gagtgccacg aagggttgga tgtccccgcg
     8701 ggatccggtg gtgatgatac catagcggtg ggttgttgtg ctggttcggt tgcggtgggc
     8761 ggggcggcgg ttcatcgttg ctcaattgcc caatgaccaa tcgatgggat cgatgcggtt
     8821 gccggccagc caggcattgg ccttcgagaa atgccggcat ccgaagaaac ctttgtcggc
     8881 cgagaagggg gaggggtgag cggccttcag gacgaggtgt ttggtttcat ccacgagcga
     8941 ctgtttttcc tgtgcgaact tgccccatag gagaaagacg gtatgttcct tgagtgccga
     9001 aactttgctg atgacggcat cggtaaattc ggcccaacct attttggaat ggctcatggg
     9061 ctcgttggcc cggacggtca gggacgcgtt gaggaggaga acgccctgtt ccgcccaatg
     9121 ggtaagattt ccgtgtgacg ggatggccat gccgacgtcc tggttcaatt ccttgaagat
     9181 attgatcagt gaaggaggcg gggggatgcc tttgggcacc gagaagctga ggccatgggc
     9241 ctgaccgggc ccgtgatagg ggtcctggcc cagaatgacg acttttagct tatcgaaagg
     9301 tgtggtattg aaggcattga aaataagact gccgggaggg tagatcgtct ttccgctgat
     9361 cttttcgttt ttcagcaggg tgacgatctg taggaagtag ggtttggtaa attcctgccg
     9421 gagggcttct ttccaactgt cctcaatttt tacatccata cgggtggctc gctttggtgt
     9481 gggccgaagg taaataaaag catgggtttg ggtcgaagcg ggattttttt tatttttgcc
     9541 ggctgttttg tggtgctgcg gtcgcccggc tttccggggg tgccgcgggc acgatacgat
     9601 aaccgacccg gtagctcagc tgaatagagc tcctgccttc taagcaggtg gtcgtgggtt
     9661 tgaatcccgc ccgggtcacg attatatctc cacttcttaa aacccgctct ggcagcgggt
     9721 tttttggttc gatacattta gtagtcaatc tcgtcaattc ctcaagcgtt ttattgtcat
     9781 gaacttaaag gtttgaatcc tgcatggtcg gccataattt aaaatccaca tttaacggcc
     9841 gccaaaactt tcaagcaaat acaaaaaatt ggcaccgcca ggtgccgcga agcgatcagc
     9901 ggagccaatc aacccaagtc ataaagccga tcagacaaat tctagccggc tttatctctt
     9961 tttttacgag tgtctcgtcc taactggtca gacctgttcg cactgacatg tctggaatgc
    10021 aattgattga ataagtgtat attaattacc tttatagagt tgaacaatcg acatgcccaa
    10081 tactccggaa tatattcctc aacttgtagg cgagatttat cgtgccaggt cgcgctcggt
    10141 gtttgccacc ctgattcgtt tgcttaaaga ttttgatctt gccgaagacg ccatgcacga
    10201 tgcttttaag gctgcacttg aacaatgggg tgaaaaaggc attccttcta acccgctagc
    10261 gtggcttgtt tccgccggcc ggtttaaagc aatcgatcgc atccggcgcg aatcaaaatt
    10321 cgactcgttg gaggaacag
//
LOCUS       DASYSV010000106         9732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_21394, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000106 DASYSV010000000
VERSION     DASYSV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21394"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VGQ51_03350"
     CDS             <1..999
                     /locus_tag="VGQ51_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEV9035624.1"
                     /translation="GSFLGDASHFVFNDHMEVVMEKKHGGFYQTALFNGKVMESERFG
                     IVIGSGRKGQSYLYWDAGRLLQLPISYYTPLDSWCNSPGYPPTMADFFKQVHGECMEC
                     HGTYASAAQREDGTTIFDSASIVYGIDCERCHGPGAEHVSWHTAHPGAIAGKYILNAR
                     HLARQQRLDACALCHSGFRQEILPAFSFKVGDTLDNYSSAGYDTAAVSNLDVHGNQYG
                     LLTASRCFRESDQLDCSTCHDPHADEHGKMEVYSSRCAGCHNGTSLHSCSYAPVIKMR
                     LTTNCIDCHMPALPSNVITLQVSGQEQPVHDLVRTHRVAVYPRISAEWLREHPRGQ"
     gene            1066..2274
                     /locus_tag="VGQ51_03355"
     CDS             1066..2274
                     /locus_tag="VGQ51_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV9035625.1"
                     /translation="MSEKHLLYKKRIIRELYFAGNLSCSDISDRTKTSLPLTTKIVSQ
                     LVEEKYVGTMGYAPSTGGRRPLIYSLLPGSLYVVSVAMDQLFTRVVVMDMHRKYVTPI
                     GKFEFSLADKNATADRLAEIISQFILESGIRSEQIAGIGIGMPGFVDPGRGINYSFLK
                     TADKPLAQFITEHTGIETFLDNDSGLIALAEFRFGAARNRKNAMVINLGWGIGLGMIL
                     NGEYFRGHNGFAGEFSHLPLYSNNKLCSCGKSGCLETEASLLEVVKKAEQGIREGRLT
                     TLSQHLPAKNVDLASRAVLSSAEEGDKFAVGLLSDAAYTIGRGVAILIHLLNPETIVL
                     SGRGSSAGKIWQTPIQQALNEHCIPRLAANTHIEISTLGYHAELVGAAALVIEHYYHP
                     EKKRPARVTQ"
     gene            complement(2343..3131)
                     /locus_tag="VGQ51_03360"
     CDS             complement(2343..3131)
                     /locus_tag="VGQ51_03360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV9035626.1"
                     /translation="MKKQVSFLGLTILFFAAGAAPLQGGNVPPPSVIMGRKEVPVLCY
                     HQVRDWKPTDSKTNKDYIMPPADLRAQLKMLHDSGYHTILPDQLYAWLTKGTALPSKP
                     IMLTFDDTDEDQYAVAWPEMKKYGFRGVFFIMTISLNKLPHYMTVGQVKDLYAAGNVI
                     GSHTWDHHNVKKYQGKDWEIQIDKPTKQLEAITGAPIRYFAFPFGLWNRQALPELRKR
                     GFIASFQLADKMDPTDPLMTIRRIIVGGGWSLKTFNTAIHRDFH"
     gene            complement(3148..3630)
                     /locus_tag="VGQ51_03365"
     CDS             complement(3148..3630)
                     /locus_tag="VGQ51_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV9035627.1"
                     /translation="MSEDLKIAGGSKTEQYASLLPQIRALMEGESDGTANLANMTAAL
                     KEQFGWLWVGFYLVKNGELVVGPFQGPVACTRIRKGRGVCGTSWAEAKTLIVPDVTAF
                     PGHIACSSLSRSEIVIPINAGGEIVGVLDADSSELNAFDEEDGRWLEEMVKLIPGIND
                     "
     gene            3679..4761
                     /locus_tag="VGQ51_03370"
     CDS             3679..4761
                     /locus_tag="VGQ51_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV9035628.1"
                     /translation="MPRFFIDPDIAKAATLPSDFYTDAQLFELSKEKLFAPSWQFIGD
                     SDQVAGPGDCRPMMLLEPYLDEPLVLVRDQESELRLLSNVCTHRGNLVVNEPCRLTHL
                     RCRYHGRQFSLDGRFRSMPEFREVENFPTANDDLPQSGLASWGKWLFASLGTGDGFGQ
                     YWGDLMRRLHWLSLDSFVHRPDLSREFTVKAHWALYCENYLEGFHIPFVHAGLNAVID
                     FGNYTTELFGLSNLQLGIAKDDEDCFDLPRDSPDYGKKVAAYYFFVFPNMMFNFYPWG
                     LSVNVVRPVTLTETRVVFSTYIADEQKYNKGAGSGLDAVELEDEEVVESVQKGIRSRY
                     YTRGRYSVTREQGTHHFHRLIAERIR"
     gene            4763..6196
                     /locus_tag="VGQ51_03375"
     CDS             4763..6196
                     /locus_tag="VGQ51_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV9035629.1"
                     /translation="MAQSLKKNIGLWSAVSIVIGGIIGAGIFMRPASMAERLGSPVLL
                     LVVWLVGGAVSLVGAMIYAELGTMFPDTGGPYIYLQKTYGDFTAFLYGWSTMAVINTA
                     AIASIAFVCAQYAGFFIHLPRLPPEIEHSVKLHIPYLADIYPLENFGVKLLTSGIIVV
                     LILVNYLSTRYGNAIQVVTTVMKTLALTLLIGGILLSGKGHPANFVTSSPGFHPGSLL
                     LMVGFMGAISGAFSSYDGWNNVNMVAGEIENPQRNISRSLFIGVGICIAVYVLTTLAY
                     NYVLPVAEMAKSPLVAADAMEKVVGVAAAGLVSGLIAFSAFGATHVNLLTNARITFAM
                     GEEGSFFPWTGVVHRRFGTPGNAVIVIGAWSILFVWSGSFDILADMFVFMSWVFYGLT
                     GLGLFILRKRIPEATRPYRVWGYPWLPLVFVAFTLFYLVTTIYGDVAAYRSGKSPVIN
                     SVFGLALTAAGIPFYLYFQRKRRNHEK"
     gene            6186..7508
                     /locus_tag="VGQ51_03380"
     CDS             6186..7508
                     /locus_tag="VGQ51_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9035630.1"
                     /translation="MRNKMYAILAACLLFATNAAHAQVADPHGRIGAGHRQTGHDSVY
                     LLRPDRVFDGEQLHEGWVVLVRGREIEAAGPSASVQAPAGVIILDLKGATLLPGLIEG
                     HSHLFLHPYNEVSWNDQVLKESRAERVARAVVHARKTLMAGFTTTRDLGTEGAGYDDV
                     GLKQAIEKGVVPGPRILCATRAIVATGSYGPKELAYDIESPHGAAEADGLEGITREVR
                     TQIGKGADVIKLYADYRWGINGASEPTFTVEEMKKAVEVASSSGRIVAVHTGTTEGMR
                     RAIEAGVTTIEHGDGGTPELFAAMKAKGIALCPTLAAGDAILQYAGWRKGVDPEPERI
                     KAKRASFQAALAAGVTICMGGDVGVYPHGDNAREMEDMVDYGMKPVDVLRSATSINAD
                     VFRISKRVGRIRAGLLADILVVEGDPAVNISQVRKVIWVMKDGVIYRP"
     gene            complement(7573..8565)
                     /locus_tag="VGQ51_03385"
     CDS             complement(7573..8565)
                     /locus_tag="VGQ51_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035631.1"
                     /translation="MKLPQLLSQYLYQHRKLDLPGIGSFTLDAGAVIPQESDRIGQVP
                     ATGISFKNANIALADDVLITYIKEHTGKMKSLAAADLDFYLTTGRQLLNIGKPFYLDG
                     IGTLLKNKEGLLEFTPGDYLVARLEDHHHDRRTTAGHEEETREESRNTNSRQTLLLVG
                     LIAGLIVIGWGGYYLYKRNNFVEPTSENRATVIPDTTAARRVDSTTVARTAVDTNTAA
                     RNASAAVLEKKDTARVDATAQKPVVTPPPDLHDPVPGAGEALYRFVILQTANKGHALR
                     RYNQLLGYKLNIRMYQKDSAFFKVYFPIAASVHDTTRIKDSLADTYEAHVTIEQ"
     gene            8663..9073
                     /locus_tag="VGQ51_03390"
     CDS             8663..9073
                     /locus_tag="VGQ51_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEV9035632.1"
                     /translation="MMTKEETDFIRYWEANRLKRKKLSRQLLVGIPAGLLITVPIVIN
                     LTSGWYKRATMEMNSSDFNPMVLIVALLIIVAFSAIFWQRHQWDQYEQRYLELLIKRD
                     REEDRQSEHVEARSGPDEGDDPGAKQSPGENQGS"
     gene            complement(9324..9512)
                     /locus_tag="VGQ51_03395"
     CDS             complement(9324..9512)
                     /locus_tag="VGQ51_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035633.1"
                     /translation="MARSVGFYKVIDSAMNRHRRRVQSQPLAEWLHEDKGNISRKLHG
                     NEAITRNDLRDVKWGSSN"
     gene            9506..>9732
                     /locus_tag="VGQ51_03400"
     CDS             9506..>9732
                     /locus_tag="VGQ51_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HEV9035634.1"
                     /translation="MPHESISVFDMFKIGVGPSSSHTLGPWRAAQRFTDSLQEKHGLS
                     GLEKITVLLYGSLAKTGKGHGTDIAIQLGLGG"
BASE COUNT         2075 a         2615 c         2810 g         2232 t
ORIGIN      
        1 gggagttttt tgggagatgc cagtcatttc gtgttcaatg atcatatgga ggtcgtcatg
       61 gagaagaaac acggcggttt ttaccagacc gcgttgttca atggcaaggt catggaaagc
      121 gaacggttcg ggatcgttat cggatcgggc aggaagggcc agtcctacct gtattgggac
      181 gcaggcaggt tgcttcagtt gcccatatcc tattataccc cgctcgacag ttggtgcaac
      241 agtcccgggt atccgccgac gatggcggat ttctttaaac aggtgcatgg cgagtgtatg
      301 gaatgtcatg gcacatatgc gagcgcggcc cagcgggagg acgggacgac gatattcgat
      361 agcgccagta tcgtgtatgg tatcgattgc gagcgatgtc atggtccggg tgcggaacat
      421 gtgtcctggc ataccgctca tcccggagcc atcgccggca agtatatcct caacgcccgg
      481 catctcgcac gccagcaaag gctcgacgcg tgcgcgcttt gtcactcggg tttccggcag
      541 gagatattac ccgccttcag ctttaaggta ggggacaccc tcgataacta ttcatcggcc
      601 ggctatgaca cagccgcagt ctctaacctg gatgtccatg ggaaccagta tgggttgctg
      661 acggccagtc gttgtttccg ggagtccgac cagctggatt gttcgacctg tcatgacccg
      721 catgccgacg agcatgggaa gatggaggtc tattccagtc ggtgtgcagg ctgtcacaat
      781 ggaacatcgc tgcattcctg ctcctatgca cccgtgataa agatgaggtt gacaaccaat
      841 tgtatcgatt gccatatgcc cgccctgcct tccaacgtga tcacgctcca ggtgtcgggt
      901 caggagcaac ccgtccatga tctcgtacgg acccatcggg tggcggtcta tccccggatc
      961 agcgcggagt ggttgcggga gcacccccgg ggccaatgac cggccaccag ggggatcacc
     1021 ggccgctagt aaaatccggc aatatccatt atctttaatg atcacatgag tgaaaagcat
     1081 ctactataca aaaagaggat catcagagaa ctgtatttcg ccggcaatct ctcctgttct
     1141 gatatcagcg atcgcacaaa gaccagtctc ccgctgacca ccaagatcgt cagccagctg
     1201 gtcgaagaaa aatatgtcgg gacgatgggc tatgcacctt cgaccggggg acgccggccc
     1261 ctcatctatt cactccttcc gggatcgtta tacgttgtca gcgtcgccat ggatcagctg
     1321 ttcacccgcg tggtggtgat ggacatgcac cggaaatatg tcacgcccat cgggaaattc
     1381 gaattctcac tcgcagataa gaacgcgacg gccgatcggc tcgcggagat catcagtcaa
     1441 ttcattcttg aatcgggcat ccgttcggag cagatagcag gcatcggtat cgggatgccc
     1501 ggttttgtcg atccggggcg cggtatcaat tattcctttc taaagacagc cgacaaaccg
     1561 ttggctcaat tcattaccga acacaccggt atcgaaacct ttctcgacaa cgattccggc
     1621 ctgatcgcac tggccgagtt caggtttggc gccgcacgca accgaaagaa tgcgatggtg
     1681 atcaacctgg ggtggggcat cggtttgggc atgatcctga atggcgaata tttccgggga
     1741 cacaatggtt ttgcagggga gttcagccat ctcccgttgt attcgaataa caagctgtgc
     1801 tcctgcggga agagtggctg tctcgagacc gaggcctcat tgctggaagt ggtgaagaaa
     1861 gccgaacagg gcattcggga aggacgtctg accaccttgt cgcagcatct ccccgcgaag
     1921 aatgtggacc ttgcgtcgcg tgccgtgctg tcgtccgccg aagaaggcga caaattcgcc
     1981 gtcgggctgc tctccgatgc agcctatacc atcggacgtg gcgtggcgat cctgatccat
     2041 ctcctcaatc cggagacgat cgtgctcagc ggacgcggat catccgccgg taaaatatgg
     2101 caaacgccga tacagcaggc tttgaacgag cattgtatcc cacggctggc cgccaacacg
     2161 catatcgaga tctccaccct tggttaccat gcggaactcg tcggagcggc ggcgctggtc
     2221 atcgaacatt attatcatcc agagaaaaag agacctgccc gcgtcactca atgagtggcg
     2281 tcggcttagc ggccgtcgga aagcgctcat cgcgcgtgcg gaaagcgctc accgcacgtc
     2341 actcaatgaa aatcccggtg gatcgccgta ttgaaggtct tgagactcca gcctccccct
     2401 acgatgatcc ggcggatggt catcagcggg tcggtcgggt ccatcttgtc ggccagctga
     2461 aaggaggcga tgaatcccct cttccgtagc tcgggcagcg cttgccggtt ccacaagccg
     2521 aaaggaaagg cgaaatagcg gataggcgcc cctgttatcg cttcaagctg tttggtcggt
     2581 ttatcgatct ggatctccca atccttaccc tggtattttt tcacgttgtg atgatcccag
     2641 gtatggctgc cgatcacatt tcccgcggca tagaggtcct taacctgccc gaccgtcata
     2701 taatgcggca gtttattgag actgatcgtc atgatgaaaa agaccccgcg aaaaccgtat
     2761 tttttcattt cgggccaggc gacggcatac tggtcttcgt cggtgtcgtc gaaggtcagc
     2821 atgatcggtt tgctgggaag agccgtaccc ttcgtcagcc aggcgtatag ctgatccggc
     2881 aggatggtat gatagccgct gtcatgcagc atcttcagct gcgccctcag atcggcgggt
     2941 ggcatgatgt aatccttgtt tgttttggaa tcggtcggtt tccagtcacg cacctggtga
     3001 taacaaagga cgggcacttc cttccggccc atgatcaccg acggcggcgg tacgttccca
     3061 ccttgaagcg gcgccgcacc ggcagcaaaa aacagaatgg tcaacccaag aaagctcact
     3121 tgctttttca tatcgaatgt cagattttta atcattgatg cccggtatca gcttcaccat
     3181 ctcctcgagc cagcggccgt cctcttcgtc gaatgcgttg agctcggagc tgtccgcatc
     3241 cagcactccg acgatctcgc cacctgcgtt tatcggaatg acgatctccg accgcgagag
     3301 gctgctgcag gcgatgtgtc cgggaaaagc cgttacgtcg ggtacgataa gagtcttcgc
     3361 ctccgcccag ctggtcccgc agacgccccg gcccttacgg atacgcgtac aggccaccgg
     3421 accctggaac ggaccgacca ccagctcgcc attttttacc aggtagaacc cgacccacaa
     3481 ccagccgaac tgctccttca gcgccgcggt catattggcc agattggccg tcccgtcgga
     3541 ttccccctcc atcagggccc tgatctgcgg caacagagaa gcatattgct cggtcttgct
     3601 gccgccggct atctttagat cttcggacat gcgcgcaaag atactatgtc tgcgcaatct
     3661 ttcctatatt gggagaccat gccccgattc tttatcgacc cggatatcgc caaagccgca
     3721 accctgccgt cggatttcta taccgacgcg cagctgttcg agctctcgaa ggaaaagctc
     3781 ttcgctcctt cctggcaatt catcggggac agcgaccagg tagccgggcc gggtgattgc
     3841 cggccgatga tgctgctcga gccttacctg gacgaacctt tggtcctggt ccgtgaccag
     3901 gagagcgagc tgcggctgtt gtcgaatgtc tgcacccatc ggggaaatct cgtggtcaac
     3961 gagccctgcc ggttgacgca tctgcggtgc cgttatcatg ggcggcagtt tagcctcgac
     4021 ggcaggtttc ggagcatgcc cgagttccgc gaggtagaga attttccgac ggctaacgac
     4081 gatctcccgc agtcggggct cgcatcctgg ggcaaatggc tattcgcctc gctgggcacc
     4141 ggggatggtt tcggacaata ttggggcgac ctcatgcgac ggcttcactg gctgtcgttg
     4201 gatagcttcg tccatcggcc cgacctctca cgggaattca ccgtgaaggc ccattgggcg
     4261 ttgtactgcg agaactacct ggaaggtttt catatcccct tcgtccacgc cggtctcaat
     4321 gcagtcatcg atttcggcaa ctatacgacg gagttattcg ggttgtcgaa cctgcagctg
     4381 ggcatcgcca aagacgatga agattgtttc gatctgcccc gggattcacc cgactatgga
     4441 aaaaaagtag cggcgtatta tttcttcgtc ttccccaata tgatgttcaa tttctatccc
     4501 tggggcttgt cggtgaatgt ggtgcggcca gtgaccctca ccgagaccag ggttgttttt
     4561 tcaacctata tcgccgatga gcagaaatac aacaaaggcg cgggatccgg tctggacgcg
     4621 gtagaactgg aggatgaaga ggtggtggag tcggtgcaga agggtatccg ctcccgttat
     4681 tatacccgcg gacgctattc ggtcacgcgc gagcagggaa cccatcattt tcaccggttg
     4741 atcgctgaac gcatacgata gtatggctca atccttaaaa aagaatatcg gtctctggtc
     4801 cgccgtatcg atcgtgatcg gcgggatcat cggggccggc atctttatgc gccctgcctc
     4861 gatggccgaa agactcggat cacccgtgct gctgctcgtc gtctggctgg tgggcggcgc
     4921 agtctcgctg gtgggcgcca tgatctatgc cgagctcggg acgatgttcc ccgacactgg
     4981 tgggccctat atctatctgc agaagaccta tggggatttt accgcttttc tatatggctg
     5041 gtccactatg gccgtgatca ataccgccgc gatcgcctct atcgcgtttg tttgtgcgca
     5101 gtatgcgggg ttctttatcc atcttccccg gctgcctccg gagatcgagc actcggtcaa
     5161 gctgcacatc ccgtatctcg cggacattta tccgctggag aattttggtg tcaagctcct
     5221 gaccagcggc atcatcgtcg tactcatcct ggtcaattat ctcagcacaa ggtatggtaa
     5281 tgcgatacaa gtcgtgacca ccgtgatgaa gacgctggcg ttgacgcttc tcatcggcgg
     5341 gatactgttg tcgggtaaag ggcatccggc gaatttcgtt accagcagtc ccgggttcca
     5401 tccggggagc ctgttgctga tggtaggttt catgggcgcc atttcggggg cattctcctc
     5461 ctatgacgga tggaacaatg taaatatggt cgccggcgag atcgaaaacc cgcagcgtaa
     5521 tataagcagg agtcttttta tcggggtggg tatctgcatc gccgtctatg tgctgaccac
     5581 tctggcgtat aattatgtgc tgccggtagc cgagatggcg aagagcccgc tggtcgccgc
     5641 tgatgcaatg gagaaggtgg tgggtgtggc ggcggcggga ctggtttcgg gtctgatagc
     5701 attctcggcg tttggcgcaa cgcacgtcaa cctgctgacc aatgcccgta tcacgtttgc
     5761 gatgggggag gagggcagct tcttcccatg gaccggagta gtccatcggc gttttggtac
     5821 gccgggcaat gccgttatcg tcatcggtgc ctggagcata cttttcgttt ggagcggcag
     5881 tttcgacatc ctcgccgata tgttcgtctt catgtcatgg gtgttttatg gacttaccgg
     5941 gttggggctt tttatccttc ggaaaaggat ccccgaagcg acgcggccct accgggtttg
     6001 gggctatccg tggctgccgt tggtctttgt cgctttcacc cttttctatc ttgtgacgac
     6061 tatctacggc gacgtcgccg cttaccgcag cgggaagtct ccggtcatca attccgtttt
     6121 cggtctggca cttacagcgg cgggtatacc tttttatttg tattttcaac gtaaacgcag
     6181 gaaccatgag aaataagatg tatgcgattc tggccgcttg tctacttttc gctacgaacg
     6241 ccgcgcatgc ccaggtggcc gaccctcacg gccgtatcgg tgcagggcac aggcaaaccg
     6301 gacatgacag cgtatatctc ttacggccgg accgcgtctt cgacggcgaa caactgcatg
     6361 aaggatgggt ggttctcgtg cggggccgtg agatagaggc cgccggaccc tccgcatcgg
     6421 tacaggcgcc tgcaggcgtc atcatcctcg atctcaaagg cgccacgctg ctaccggggc
     6481 tcatcgaagg gcatagtcat ctctttttgc atccgtataa cgaggtctcc tggaatgacc
     6541 aggtcctgaa agaatcgagg gcggagcggg tagcgagagc ggttgtacat gctcgtaaga
     6601 ctttgatggc gggttttacg accacacgcg atctcgggac cgagggggcg ggatatgacg
     6661 atgtggggct gaagcaggca atcgagaagg gggtcgtacc cggaccgcgg atcttatgtg
     6721 cgacccgcgc catcgtcgcc acgggcagct atggaccgaa ggagctggcc tacgatatcg
     6781 aatcgccgca tggcgccgcg gaagccgacg gcctcgaagg gatcacccgc gaagtgcgta
     6841 cgcagatcgg caaaggcgcc gatgtaataa aattatatgc cgattaccgc tggggaatca
     6901 atggcgccag tgagccgaca ttcaccgtgg aagagatgaa aaaggcagtg gaagtcgcct
     6961 ccagcagcgg ccgtatcgtc gcggtacata ccggtaccac cgaagggatg cgtcgcgcta
     7021 tcgaagccgg tgtcacgaca atagaacatg gcgacggcgg tactccagag ctttttgccg
     7081 ccatgaaggc caaaggcatc gcgttgtgtc ctacgctggc agccggagac gccatccttc
     7141 aatatgccgg ctggaggaag ggagtcgatc cggaacccga acgcatcaag gccaagcggg
     7201 ccagctttca ggccgcgctc gcggcgggcg tcaccatctg tatgggcggt gacgtagggg
     7261 tgtatccgca tggcgataat gcgcgcgaga tggaggacat ggtcgattat ggtatgaagc
     7321 cggtggacgt cctgcgttcg gccacctcca taaatgcaga tgtcttccgg atatccaaac
     7381 gggtcggaag gatacgcgcg ggactcctgg cggatatcct ggtggtggag ggcgatccgg
     7441 cggtcaacat atcgcaggtg cggaaggtga tctgggtaat gaaggatggg gtgatctacc
     7501 ggccctgatc agccgctacg gatcgttacc ggtcgcccgg gatggtcccg gcgggtcgcg
     7561 ggtcacatcc ctttattgtt cgatcgtcac gtgtgcttca tacgtatccg ccaacgaatc
     7621 tttgatacgg gtagtatcgt gtaccgacgc agcgatgggg aaataaactt tgaaaaaggc
     7681 cgaatccttc tgatacatcc ggatattcag cttgtaaccc agcaattgat tataacgccg
     7741 cagggcatgg cccttattgg ctgtttggag aattacaaaa cgatagagag cctcacccgc
     7801 acccgggaca gggtcatgga gatccggcgg gggcgtgacg accggtttct gagcagtggc
     7861 atccaccctg gccgtatcct ttttttcaag gacggcggcc gaagcgtttc gggcggccgt
     7921 attggtatcg acggcagtgc gggccacagt cgtagaatcc acgcgccgtg cagcggtcgt
     7981 gtcggggatg acggtggcgc ggttctcgga agtcggctca acgaaattgt tgcgtttgta
     8041 caggtaataa ccgccccatc cgatgacaat gagcccggca atgagaccga ccagcagcag
     8101 cgtctgccgt gaattggtat tccgggattc ttcccgggtc tcttcttcat ggccggcggt
     8161 ggtccggcgg tcatggtggt ggtcttcgag acgggcaacg agatagtcgc cgggggtgaa
     8221 ttccagcagc ccctctttgt ttttgagcag ggtcccgatg ccgtcaagat aaaaaggctt
     8281 gccgatattg agcagctgac ggccggtggt caaatagaaa tccaggtcgg cggcggccaa
     8341 cgatttcatt tttcccgtat gctccttgat ataggtgatg aggacgtcgt ctgcgagggc
     8401 gatattggca tttttaaagg aaatgcccgt agcgggcacc tgtccgatcc tgtccgactc
     8461 ctgggggatc actgctcccg catcgagggt gaaactgccg attccgggta gatcaagctt
     8521 tctgtgttga tataagtact gtgataatag ttgtggtagt ttcacacaat cggattttgc
     8581 gatgggataa agataaagct tttggtgtac ttccccgtaa ctttgtggag catacggccg
     8641 aatgctgccg tcaaacgccg ctatgatgac gaaggaagag acagatttta tccggtattg
     8701 ggaggccaat cgcctcaaaa gaaaaaagct ttcccgacaa ttgctcgtag gtatcccggc
     8761 aggtttgctg atcacggtgc cgatcgtcat taatctcacc tcgggatggt acaaacgcgc
     8821 gaccatggag atgaacagca gcgatttcaa ccccatggta ttgatcgtcg cgctattgat
     8881 catcgtcgct ttttcggcca ttttctggca gcggcatcag tgggaccaat atgaacagcg
     8941 gtatctcgag ctgctgatca aacgcgatag ggaagaggac cgtcagagtg agcatgtgga
     9001 agcacggagc ggcccggacg aaggagatga cccgggggca aaacaaagcc ccggcgaaaa
     9061 ccagggctct tagcaggtag ttcatggtta ctgcacatca agcagctgta tatctaaaaa
     9121 tcaggatgaa aaaacggtat ctggcgccac acaacgataa ggttagacat tgattggcag
     9181 tctcttaagt tgatccaata aggttgatta gtgatttgtc ccgtgtaaac ccagtaaatt
     9241 agacaactcc ttagcaggaa ttgctgcaag aaaattaaaa aaacttcaag attttttaat
     9301 tctgtcctgt aattggacgc gtgttagttg gaactgcccc attttacatc cctgaggtca
     9361 ttgcgcgtaa tggcttcatt accatgcagt ttcctgctta tgttgccctt gtcctcgtgc
     9421 aaccattcgg ccagcggctg ggattgaacc cgccggcgat gtctattcat cgcggaatcg
     9481 ataactttgt agaaacccac cgatcttgcc acacgaaagc atttccgtct tcgacatgtt
     9541 caaaattggc gtcggtccgt ccagctcgca caccctcgga ccctggcgcg ccgctcaacg
     9601 gttcaccgac tcgctccagg aaaaacatgg actatccggt ctcgaaaaga taaccgttct
     9661 tctctatggc tcgctggcca agacagggaa aggacacggc acggatatcg ccatccagct
     9721 gggtctgggg gg
//
LOCUS       DASYSV010000107        18820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1685689, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000107 DASYSV010000000
VERSION     DASYSV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 18820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18820
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685689"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VGQ51_03405"
     CDS             <1..759
                     /locus_tag="VGQ51_03405"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEV9035635.1"
                     /translation="FASPASPIDYLKIPIQTLKVGSLGTHNCLGLAKSKRARILVAST
                     SEIYGDPLIHPQTEEYWGNVNPVGPRGVYDEAKRFQEAMTMAYHNFHKLETRIVRIFN
                     TYGPRMRLNDGRALPAFIGQALRGENLTVFGDGSQTRSFCYVDDLVEGIYRLLLSDYH
                     LPVNIGNPSEITLKQFAEEVIRLTGTKQSIVYKPLPVDDPKQRRPDITRAKEILGWEP
                     KVERKEGLKRTYEYFKSLPPEEWTRQPKEFVSQS"
     gene            783..2081
                     /locus_tag="VGQ51_03410"
     CDS             783..2081
                     /locus_tag="VGQ51_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV9035636.1"
                     /translation="MNESKIYEDLLTKKAQLAVIGLGYVGLPIALEFARRISVIGFDI
                     NNHRIQLMRAGVDPSKEVAPGEFRDRDITFTSDPNVLAKARFFIVAVPTPVDKYNVPD
                     LKPLIGASTTVGKVLKKGDYIVYESTTYPGCTEEDCLPLLEKHSGLSLKDGDFSLGYS
                     PERINPGDKKHTLRTVVKVVSGDTPLALEEIAKTYELVVDAGVHRAPSIKVAEASKII
                     ENTQRDLNIALMNELSLIFDRMGINTYDVIDAAGTKWNFLKFHPGLVGGHCIGIDPYY
                     LTYKAAALGYESKVIASSRYINDDMARYVARKIITHVLRISNEPKVLIKGVTFKENVS
                     DIRNSRIVDAVKELLAFNIKVDVEDPYAEEEEVHHEYGLHLTSQLRTDYDAVIITVPH
                     TQYKDLDDAYFCSITREGALVADLKGTYKNKIHNRKYWSL"
     gene            2081..3145
                     /gene="rfbB"
                     /locus_tag="VGQ51_03415"
     CDS             2081..3145
                     /gene="rfbB"
                     /locus_tag="VGQ51_03415"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV9035637.1"
                     /translation="MAEQKRTILITGGAGFIGSHVVRLFVTKYPQYRIVNLDALTYAG
                     NLANLEDIESAPNYIFEKGDITDVGLLKQLFATYRFDSVIHLAAESHVDRSIMDPLAF
                     VRTNVLGTATLLNAALNSWKDDFPGKLFYHVSTDEVYGSLGAEGFFTEETPYDPRSPY
                     SASKASSDHFVMAWHHTYGMPVILSNCSNNYGSHHFPEKLIPLMINNILHSKPLPVYG
                     KGENVRDWLYVEDHARAIDTVFHKGRVGEKYNIGGFNEWKNIDIVHLLCGIMDRKLER
                     PAGTSAGLIQFVKDRPGHDLRYAIDATKLNRELGWQPSLQFEEGLEKTVDWYLSNQDW
                     VRAVTSGDYQKYYKEQYHKR"
     gene            3149..3709
                     /gene="rfbC"
                     /locus_tag="VGQ51_03420"
     CDS             3149..3709
                     /gene="rfbC"
                     /locus_tag="VGQ51_03420"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEV9035638.1"
                     /translation="MPFRTTDIPGLLIFEPAVYTDERGYFFESYNEEVFRREGIANHF
                     VQDNQSFSGYGVIRGLHYQLEPYAQCKLLRVLQGAILDIAVDIRVGSPTYGRHIAIEL
                     SAENKWQFLIPPGFAHGFSVLTPTAEVAYKCDAFYKKESEAGIRFDDPVLDIDWRIPA
                     DKRIVSAKDLALPLLADCRNTFKLVG"
     gene            3706..4560
                     /gene="rfbD"
                     /locus_tag="VGQ51_03425"
     CDS             3706..4560
                     /gene="rfbD"
                     /locus_tag="VGQ51_03425"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV9035639.1"
                     /translation="MKTVLVTGAGGQLGRELQRLAAGHHFVFTTREQLEVDDPEQVQA
                     VFADVKPDWCVNCAAYTAVDKAESEPETAFRINGDAPGFLAKACRAAGTRLIHISTDY
                     VFDGSRNIPLKEDDPTGPINVYGASKLEGEKQVLREYSEGALIIRTSWVYSEFGNNFV
                     KTMIRLMNERPAIGVVDDQVGTPTYAADLAAAILMILSARKFLPGIYHYSNEGQISWY
                     QFALAIRFLIDSPCKVSPIPTTQFPTPARRPAWSLMDKTLIRDSYGVPILAWERSLAA
                     CINRLQKA"
     gene            4808..7759
                     /locus_tag="VGQ51_03430"
     CDS             4808..7759
                     /locus_tag="VGQ51_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9035640.1"
                     /translation="MKPVYPTRVFSLLVNLIRHRRFAAALLILSGIAYSTRIVAEGTK
                     QVMPSSTNGTGLIVSTTASFPLGNVGSYLGAPVDARVYIYVKDFTTEKVYYGFNWETL
                     SPATPINTYSDVYMNIYNPTGALISTVHLPSTTGSAGFISSYASAVAGPQIGGLPLSG
                     YVPLSFTPTMNGDYYVSFYRSSDGGVTHLAGGESMLSKYFDITVAASAASKFAGRVHC
                     NEWAMSVYNPAKNDIQDPQSPTNAAFFGYTPDSVTVKVSFPNNGFMPLTFIVAFNSFG
                     VTNSGNWLVDRNSINLPNLVSPYLTGGFKVFLNAPDASIYPACSIPPPPVLLAPVISG
                     CPPGPYNVRFMAPQAGDYYMLFDLNGVPGFQNNTADRFIELVNQSPGVITYVWDGKDG
                     LGNVVPANTTFPINFSYRKGRINIPFYDVELNQLGFRVDGVSPAGAVVSNTTLYWNDT
                     QLSSMGTDCSNNNNNTTGTGYNNSVVGVTPVWPQTPAVPTYGRAWSGDGNPLNVTPAD
                     SVLVSGTYNNHDAQQCDDYGNARLLNTWAWGIVLNTTQNLTLTCITVSGTVWDDADGS
                     AAGGFTNIRTNSEPGTNAGGGLYANLVDPVTGDVLSSVAVNTDGTYSLANCPVNATGM
                     EIYLTTTQGVIGSPVATAAVPSAWINTSPMIRTFNSTNTDVTGQDFGIEQLPNSVDQT
                     YTIPVPVQNSLMSLNGFSTIASPGPLAGSDPEDGTLGSGKSVVITTVPANEQLYYNGV
                     LVTNNFVITGYNPFLLQMKFTDISVTATSFTYAFWDAANKQDPTPATYSINMSVALAA
                     TLSSFTGRATEAGNVLNWTDQSETPDVTFYIQRSVDGLNFTTIGQVAAASGTGSGGGS
                     VNHSFTDNSPTPNTPNEYRLEWTDGRGDVVYSNVVTLVNSLASGLQDVSPNPFRDQVT
                     VRLSLGRTQPVTIRLLDSKGQLLRDRQYAGMKGANSFVLDGLSALPSSVYLVQIVLTD
                     QVFVRKVFNNR"
     gene            7828..9630
                     /locus_tag="VGQ51_03435"
     CDS             7828..9630
                     /locus_tag="VGQ51_03435"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV9035641.1"
                     /translation="MKILFPVAALLLLAEAGVHAQSSMSVTSTEVNNILLGSYTPGNY
                     TASTVITDPATIAAGLLNDISADSLKADLFALNAFHNRNMFSDTTSTTTGIGATRRWI
                     YNKFLQYSAVNDNRLRPAYLKFTYAPSTYGCTGVGSNSTQYNVMAVLPGSQKTNPSLI
                     IIEAHMDSRNSSNCDVTGSAPGIGDNATGTALVMELARVLSKYTFRYTIVFSVNTGEE
                     QGLIGAHALADYLDSKNVLIKAVNNNDVSGGVFCGHTASAPSCPAYGNIDSTDLRIFS
                     LGDVNSANKQWARYIKLEYKEMLSSLVSVPTNIDIMEADDRTGRGGDHQAFTKDGYTA
                     VRFTQANEDGNASNGPGYSDRQHNVRDSLGHYNSVTHTIDSIYVNVDYLARNALVNGN
                     ALAMAALGPDTISMSGTLWTAGTVKVKFSPTSSPGYRVAVRSASNDWDTVYTVTGMST
                     AVLTLPYSSNTTFYISAAAMDANGTEGQFSTEYTLSTGLIPLDLLPDTTKPVPTDPDY
                     YGIRLMPNRPNPFDESTMIAITSGTDMFAARTCLQIADIEGRVIARIPVHLVTGLNQV
                     IYQHGFGVSGMYICSLVIDGLPVQSTKMIFRGSH"
     gene            9806..11446
                     /locus_tag="VGQ51_03440"
     CDS             9806..11446
                     /locus_tag="VGQ51_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035642.1"
                     /translation="MKHRIVLLFFLFCSSKLFCQHGTLTLSNDFKIAEGQYKHETVTN
                     SVYFNNNFYTVTNSAPSAGKWLFTKLYDVKFSLTVSEFDKDMNKIREVVLENGDKNFG
                     PLIPKLISFHDRLYLTYFKAADKTSFSVYLAALDENNLTLGEPKLICTIKQDNVGLGQ
                     IESVINAGLVYFVISPDNARLLAVCKAAPNKVQAVLLDDKLATIKQSLVSVNLQDFEI
                     PSAVLTNDNRACLVLDSKDGTRLLCIGPDGAKTEIRYNAAASFAPYITRARLVRDGKS
                     IIIYSTTIESGGSDSWCTGIMVARLDPATFRLSKPLTYALTPEFIQAISEQGGGTKHK
                     HDYFMSNFTPQLLELDNGDVAIIGSPEMISEKSSTSAPNMNGQTHEIATTTVTVGPVI
                     VLFPDKNGKTYNPVIVPREIILSKSQSSGSGAIRVVASPRQSDVSTGFLATQIGGDIV
                     IIYNDNVKNLSKDAGEKLTVSKSPGDLELAEALVNKDKKLEYRKLISETTKGRATFYL
                     GSSVPSNSSSIVFPIGKEGQGFNALKTYYTNWCFLEIK"
     gene            11493..12074
                     /locus_tag="VGQ51_03445"
     CDS             11493..12074
                     /locus_tag="VGQ51_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV9035643.1"
                     /translation="MEELLSFLNQIASSEGWPLSAACLAHLGEIIKIRTLKQEELLLS
                     AGQVCKNIYFIRRGLLKCFYIVHEREVSDWFFGELETVVSIDSFYDQVASEDFIQALE
                     DCELFCISFGELNYLFRTFVEFNVVGRVLFNKYLRIWHRQARNIRMLTAEERYQFLLT
                     TQPNLIQRVPVRDLASYLDMSRETLSRMRRRIQ"
     gene            complement(12082..14064)
                     /locus_tag="VGQ51_03450"
     CDS             complement(12082..14064)
                     /locus_tag="VGQ51_03450"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YWFCY domain-containing protein"
                     /protein_id="HEV9035644.1"
                     /translation="MQPANDNQYRELRNLAACCCAALLILHYYYFCHRGLATWSLTCP
                     IADRLVTGIASKGLFRHPAVSLGLALLALAGTSIGAKTPPQIKSGRLIVILIAGIVLY
                     FGSCLILYVDADPRMLTIVYAVTTVCGLGLLYTSVRALTGLVTWPAKRGLFNQYNESF
                     PQEERRLTHPNFFHLKGRYRFQNRWRNSVINLDIYRGTLVMGSPGSGKTRYVFKQLIR
                     QSIDQGMAIFIFDLKYPGLTEFTYQQLESVRAGLKNPPKFYSFNFNTLETRCNVLAPE
                     TLQELSDAMECARTLYFGINRKAAKSKGDFFVESAVNFFAANIWFLRQYEEGRYCTLP
                     HLLELMSTDYAHLFSVLRSYPDIATLVKPFTDALQHNVMDQLQGQLDSARIPLFSLVS
                     PKLYYLLSGSDLNLDINNPNAQKIVCLGSDPERQHIYGAVISLFVSRMLKRINRPGGT
                     PCQVYIDEFPSFHVLNMDTILATCREHKVGIVLGIQTIDQLRAEYGREHAEALVNLPA
                     NLLTSQVTGDSAKFTSEQIGKILQERSTISTNNRGTSTSQSLQLDLAMPASKIAALSS
                     GEFVGITADSPDRPMPLKAFHCQVKLEPQPDNVNNPRPSQKVSLETVNQVFQQIQEET
                     KRIVKNRLNEMRNNPTRARLILNPKVGARRRKQTKL"
     gene            14272..14937
                     /locus_tag="VGQ51_03455"
     CDS             14272..14937
                     /locus_tag="VGQ51_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9035645.1"
                     /translation="MRERERPCLHILVADQHRIFRAGIRTLLAEAGSWQAFEVGEAET
                     TEEAIAIVATGRYPVVLIEYNLPGRGGIKAMEIIRSRWPAIAVIAVVETDDGGPAERM
                     IDAGAKGCILRNVGLDTLVGAIRTVMGGRRFFSNEIAQRLLERPRRAKQDPLERLTAK
                     EKQVFHSILAGLRDREIAAQMGIAKRTVDKHRQHIHYKLGTKNALELLQTGLRLGLVR
                     GPE"
     gene            15492..16694
                     /locus_tag="VGQ51_03460"
     CDS             15492..16694
                     /locus_tag="VGQ51_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035646.1"
                     /translation="MKRFFFLLLIVTIVSMNNSFAQTTFWSVTGNTGQRGDTSFVGTI
                     DSNRLAFRTKNREHMSISPTGTVAVGTETELDSLTLNGPMYIWDSVGIDPLSIESNND
                     YGSFVNFTNASTYYAASIGLRFYNSAGIVSQMFVGSPSSIYAPNGFGFQDLSGGGFDF
                     SSRIAGSFFSWGSDFKAAGGSKMIFYTTPGHLIIGSTTDSGNSKLQVRGNAWMQGLTL
                     PTGAASGYVLTSDTAGNASWQPGSSGRWAFANGSVFDSSDNIAIGTSNPQGYKLAVNG
                     TAMFTKIKVRPLSSWPDYVFRKGYVLPGLEELEAYVNQYHHLPGITSEDEVKQDGIDL
                     GEQHTALLKKVEELTLYLLNENRSLKDQIKELSEQKSQLAEQNARLQAQQKEIDDLKA
                     MIQANTKH"
     gene            16705..18171
                     /locus_tag="VGQ51_03465"
     CDS             16705..18171
                     /locus_tag="VGQ51_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035647.1"
                     /translation="MKKILVACAVGLSLNIGISRAQSVDSAAGKVVSFPSRLFSRIQS
                     RTARLNNQLTSQTQKYLQKMARREQQLQRRLASVDPAAAQRLFAGSAQRYAVLEQQIK
                     ADTGGSGRSFSGVYMPYLDSLQGSLAFLQKNPQLLPNPGVGQLTQLQAAGGQVKSYEA
                     KLMDADQVKAYIQQRKQMIGQYITQHANLQGLLGKSYAGMNQDVYYYSQQLRAYKEMW
                     NNPDQLLQKALVVLNRLPTFQSFMKGNSMLGTLFNLPGNYGSPQAISGLQTKDQVAQQ
                     IQGHVSAGGTGGAAALQSNLQLAQSQLAGYKSKLSQLGSGNGDMDMPNFKPNDQKTKT
                     FWKRLEYGADFQTTRNNYYFPMVTDFGLSLGYKLGHNNIIGVGASYKLGWGNGIQHIA
                     FTNEGVGLRSFLQIAVKGSFSATGGFEYNYTTPFTSYQQLKQIQYWTKSGLIGATKTV
                     SLKSRVFKKTSLSLLWDFLSYQQVPRTQPILFRIGYNF"
     gene            18191..>18820
                     /locus_tag="VGQ51_03470"
     CDS             18191..>18820
                     /locus_tag="VGQ51_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035648.1"
                     /translation="MRRVILFLLFSGVSLFGYTQSTTDPTQGNINVVTPRTPESAGFE
                     KFGKYDVNEFTGTANISIPLYTLSSRHLAVPITLSYHPTGIRVNEEASWVGLGWDLIA
                     GGRITVETKGSVDFATGSVGGNGPSSTANEMQRIFKHFDGGGELGIATFPTFFETDVG
                     SSGQLASDVDSNLDITSGVQDMDEFGTGEPDIFRANFMGHTFNFYFDKIT"
BASE COUNT         4498 a         4947 c         4948 g         4427 t
ORIGIN      
        1 ttcgcctcac cggccagccc catcgactat ctcaagatac cgatccaaac gctgaaagta
       61 ggttcactcg gcacccataa ttgccttggc cttgcgaaat ccaaaagggc gcggatcctg
      121 gtggcctcca cttcggagat ctacggcgac ccgctgatcc atccgcaaac ggaagaatac
      181 tggggcaatg tcaaccccgt cggcccccgc ggcgtctacg acgaagccaa acgcttccag
      241 gaagccatga cgatggccta tcataacttc cataaactcg agacccgcat cgtaaggata
      301 ttcaatacat acggaccgcg gatgcggctc aatgacggca gagcccttcc ggcttttatc
      361 ggtcaggcgc tgcgaggtga aaacctcacc gtcttcggtg atggcagcca gacccgcagc
      421 ttctgctatg tggacgacct ggtggaagga atttaccgtc tcctgctgag cgattatcat
      481 cttcccgtca atatcggcaa cccttcggag atcaccctca aacaattcgc tgaggaagtg
      541 atcaggctga cggggactaa acaatccatc gtctataaac cgctgccggt ggacgatccc
      601 aaacaacgcc ggcccgatat cacccgcgca aaagagatcc tcggatggga gcctaaggtg
      661 gagaggaagg agggtctgaa acgcacctat gaatacttca aatcgctgcc tcccgaggaa
      721 tggacccggc agccgaaaga attcgtaagc caaagttaaa atcaactcag ttctccaata
      781 ccatgaatga atcgaagatc tacgaagacc tcctgacaaa aaaagcgcaa ctggccgtca
      841 tcggtcttgg ctacgtaggg ctgcctatcg ccctcgaatt cgcgcgccgg atatccgtta
      901 tcggctttga tataaacaac caccgcatcc agctgatgcg cgcaggcgtc gaccccagca
      961 aagaggtcgc ccccggcgaa ttcagggacc gggatatcac atttaccagc gatcccaatg
     1021 tcctcgcaaa ggcccgcttc tttatcgtag ccgttccgac tcccgtagat aaatacaatg
     1081 ttcccgatct gaaaccgctg atcggcgctt cgaccactgt cggaaaggtg ctcaaaaaag
     1141 gcgattatat cgtctatgag tccaccacct atcccggatg taccgaagag gactgtctcc
     1201 cgctgctgga aaagcattcc gggctcagtc tgaaggacgg cgatttcagc ctgggctatt
     1261 cacccgaacg catcaatccc ggcgataaaa aacatacgct gcggaccgtc gttaaagtgg
     1321 tctccggcga caccccgctc gcgctcgaag agatcgcaaa gacatatgag ctcgtcgtcg
     1381 atgccggcgt tcaccgcgct ccgagcatca aggttgccga agccagcaag atcatcgaaa
     1441 acacccagcg ggacctgaat atcgccctca tgaatgaatt atcgcttatc tttgaccgca
     1501 tggggatcaa tacctatgat gtcatcgatg ccgccggcac caaatggaat tttttgaaat
     1561 tccaccccgg tctggttggc gggcactgca tcgggatcga tccctattac ctcacctata
     1621 aggctgccgc acttggttac gaatcgaagg tcatcgcttc gtcgcgctat atcaacgacg
     1681 atatggcacg ttatgtcgcc cgcaagatca tcacccacgt cctccgcatc tcgaatgagc
     1741 caaaggtgct gatcaaaggc gtgacgttca aggagaatgt cagcgatatc cgcaactcgc
     1801 gtatcgtcga tgcagtgaag gagctgctgg ccttcaatat aaaagtggac gtcgaggatc
     1861 cgtatgccga agaggaagag gtgcaccatg aatatggact gcatctgacc agccagctca
     1921 ggaccgatta cgatgccgtc attattactg tcccgcacac ccaatataag gatctcgacg
     1981 atgcttattt ttgctcgatt acccgtgaag gcgcccttgt cgctgacctg aaaggaacct
     2041 ataagaataa gatacacaat agaaaatact ggagtttgta atggcagagc agaaaaggac
     2101 tatcctgatc accggcggcg ccggctttat cggctcgcat gtagtgcggc tgttcgtcac
     2161 caaatacccg caataccgca ttgtcaatct cgacgcgctc acctatgccg gcaacctggc
     2221 caatctggaa gacatcgagt cggcacccaa ttatattttc gaaaagggcg atattaccga
     2281 tgtcgggctg cttaaacagc tgttcgccac ctatcgcttt gacagcgtga tccatctcgc
     2341 ggcggaaagt catgtagacc gcagtatcat ggacccgctg gctttcgtac gcaccaacgt
     2401 attgggcaca gccaccctcc tcaacgcagc actcaattcc tggaaagacg atttccccgg
     2461 caagctcttc taccacgtct ccactgacga agtatacggt tcgctgggcg ccgaagggtt
     2521 cttcaccgaa gaaacgcctt atgatccgcg ttcaccctat tcggcctcca aagcctcttc
     2581 ggaccatttc gtcatggcat ggcatcacac ctatggcatg ccggtcatcc tctccaactg
     2641 ctcgaacaat tatggcagcc accattttcc cgagaagctg atcccgctga tgatcaacaa
     2701 tattttgcac agcaaaccgc tgcctgtcta tgggaaaggg gagaacgtcc gcgactggct
     2761 gtatgtcgag gaccatgccc gcgctatcga taccgtcttt cataaaggaa gggttggcga
     2821 gaaatataat atcggcggct tcaacgaatg gaagaacatc gatatcgtcc atttgctgtg
     2881 tgggatcatg gaccggaagc tggagcgtcc cgcaggcacc tcggcaggac tgatccaatt
     2941 tgtcaaagac cgccccgggc acgatcttcg ttatgccatc gatgcgacga aattgaacag
     3001 ggaactggga tggcagccgt ctctccaatt cgaagaagga ttggaaaaga ccgtcgactg
     3061 gtatctttcc aaccaggact gggtccgggc agtaacctcc ggcgactatc agaaatacta
     3121 taaggaacaa taccacaaaa gataaggaat gccgttcagg actaccgata tccccggact
     3181 gctgatcttc gagccggcag tatataccga cgagcggggc tactttttcg aatcctataa
     3241 cgaagaggtc tttcgccgtg agggcatcgc caatcacttt gtgcaggaca atcaatcgtt
     3301 ttccggctat ggggtgatcc gcggtcttca ttaccagctg gaaccctatg ctcaatgcaa
     3361 attgctgcgg gtacttcaag gagccatatt ggacatcgcc gtcgatatcc gcgtggggtc
     3421 tcccacttat ggccgccaca tcgccatcga gctttcggcc gaaaacaaat ggcaattcct
     3481 cattcccccc gggttcgcac acggattttc cgtgctgacg ccaaccgccg aggtggccta
     3541 taaatgcgat gccttctata aaaaggaaag cgaggccggc atccggttcg acgaccctgt
     3601 gctcgacatc gattggagga tcccggcgga caagcggatc gtttcggcca aggacttagc
     3661 attgcctttg ctcgccgact gccggaacac ctttaaacta gtcggatgaa gacagtactc
     3721 gtcaccggcg cgggcggcca gctaggtcgc gagctgcagc ggctggctgc ggggcatcac
     3781 tttgttttta ccacccgcga acaacttgaa gtggatgacc ctgagcaggt acaggccgta
     3841 ttcgccgatg tcaaacccga ttggtgcgtc aactgcgcgg cttataccgc ggtcgacaag
     3901 gcggagagcg aaccggagac cgccttccgc atcaatggcg acgctccggg tttcctggca
     3961 aaggcctgcc gggctgccgg cacgcggctg atccatatct ccaccgatta tgttttcgac
     4021 ggcagccgga acatcccatt gaaagaagac gacccgacag gtcccatcaa tgtctatggc
     4081 gccagcaagc tcgaaggcga aaaacaggta ctgcgcgaat acagcgaagg cgcattgatc
     4141 atccggacct cctgggtgta ttccgaattc ggcaacaact tcgtgaagac gatgatccgg
     4201 cttatgaatg agcggcctgc tatcggcgtc gtcgatgacc aggtaggtac acctacctat
     4261 gcggctgatc ttgccgcggc gatcctaatg atcctttctg cccggaaatt ccttccgggt
     4321 atctatcatt atagtaatga aggacagatt agctggtatc agtttgcatt ggcgatccgg
     4381 ttcctgatcg acagcccgtg caaagtgagc cctattccta ccacgcaatt ccccacaccc
     4441 gcccgccggc cggcctggtc gctgatggat aagaccctta tccgggacag ctatggagtg
     4501 ccgatcctgg cctgggaacg cagtctggcg gcttgtatca accgattgca aaaggcatga
     4561 ttttcccatt atcccgcact aagtgttttt actgatgtgg aaaactaaac acgtattaaa
     4621 attaatttat ttaatgcgta agtcattgtt tactaaaaca tatagaactt atttgtcctt
     4681 ttagagtagt ctacagaatt tgtgaggata ggaccatcgg ataaaagatg ataatttgca
     4741 ttcaggtctt aaatcccgtc gacgaatgct tgggtcgccg gggattagtc cactttaatc
     4801 catacttatg aaacctgttt acccaaccag ggttttcagc ctgcttgtga atttgatccg
     4861 ccatcgccgt tttgcggccg ccctgctaat cctttccggg atagcgtata gcacgcggat
     4921 cgtcgccgaa ggtacaaagc aggtgatgcc ttcctccacc aacggtaccg gtctcatcgt
     4981 ttccaccacc gccagttttc ccctcggtaa tgtcggcagc tatcttggcg cgcccgtcga
     5041 cgcccgggtc tacatctatg tcaaggattt taccaccgaa aaggtatatt atggcttcaa
     5101 ttgggaaacc ctgtctcccg ccacgccgat caatacctat tcggacgtgt atatgaatat
     5161 atataacccg acgggcgcac tgatctcaac cgttcatctg ccgtcgacca cgggtagcgc
     5221 gggttttatc agcagttatg ccagcgcggt ggccggacct cagatcggcg gtctgccgtt
     5281 gtcgggttat gtcccgctga gctttacccc gaccatgaac ggggactatt atgtgtcatt
     5341 ctaccggagc agcgacggcg gtgtaacgca tctcgccggc ggggaatcca tgctgtcaaa
     5401 atatttcgat attaccgtgg ctgcaagcgc agcatccaaa tttgccggcc gtgtgcattg
     5461 taacgagtgg gccatgtccg tttacaaccc ggcaaagaac gatatccagg atccccagtc
     5521 gcctacgaat gcagcattct ttggctatac tccggactcg gtgaccgtga aggtctcgtt
     5581 ccccaacaat ggattcatgc cgctgacctt tatcgtggct tttaacagtt ttggtgtgac
     5641 caatagcggc aactggctcg tagaccggaa ttccatcaat ctgccgaacc tggtatctcc
     5701 ctatctgacc ggcggtttca aggtcttcct caacgcgccc gatgcctcca tttatccggc
     5761 ctgctcgatc ccgcccccgc cggtgttgct cgctccggtg atctcaggtt gtccacccgg
     5821 accctataat gttcggttca tggcgcccca ggccggtgac tattatatgc tgttcgatct
     5881 gaacggcgtc ccgggttttc aaaataatac agccgaccgg tttatcgaat tggtgaatca
     5941 gtcgcccggt gtcatcacct atgtctggga cggcaaagac ggtctgggca atgtcgtgcc
     6001 ggccaatacc acctttccga tcaatttctc ataccgcaag ggtcgtatca atatcccttt
     6061 ttatgatgtg gagctgaacc agctgggttt ccgtgtcgat ggggtatctc ctgccggcgc
     6121 ggtggtgtcc aatactaccc tgtattggaa tgacacgcaa ctcagcagta tgggtaccga
     6181 ctgttcgaac aacaacaaca atactaccgg caccggttat aataattccg tagtcggcgt
     6241 tacgcccgtt tggccgcaga ccccggccgt ccccacgtat ggacgcgcct ggagtggcga
     6301 tgggaacccg ctgaatgtca cgccggccga ctcggtgctt gtcagcggca catataacaa
     6361 ccatgacgct cagcagtgtg atgactatgg gaatgcccgt ctgctcaaca cctgggcatg
     6421 gggtatcgtg ctcaatacca cgcagaacct gacgcttacc tgtatcaccg tcagcggcac
     6481 ggtttgggat gacgccgacg gcagcgcggc gggcggtttt acgaatatcc gtacaaactc
     6541 ggagcccggc accaatgcag gtggtggtct gtatgccaac ctcgtggacc cggtgaccgg
     6601 cgacgtcctt tcttcagtcg ctgtgaacac cgatggtacg tattcactgg ccaactgccc
     6661 cgtgaacgcc acggggatgg agatatatct gacgacgacg cagggcgtga taggaagccc
     6721 ggtggccacc gccgcggttc catcggcctg gatcaatacc tcgccgatga tacggacctt
     6781 caactcgacc aatacagatg ttacgggtca ggatttcggc atcgagcaat tgcccaattc
     6841 cgttgaccag acctatacaa tcccggtccc cgttcaaaac tctttgatgt cactgaatgg
     6901 gttcagcact atcgccagtc ccggccccct ggctggcagc gaccctgagg atgggaccct
     6961 gggtagtggc aaatcggtgg tgatcactac ggtgcctgcc aacgaacagc tgtactacaa
     7021 tggcgtactc gttacgaaca atttcgtcat taccgggtat aatccgttct tgctgcagat
     7081 gaaattcacg gatatttcgg tgaccgcgac ctcctttacc tatgcgtttt gggatgcggc
     7141 taacaaacag gatccgacgc cggctaccta ttccatcaat atgtcggtag cgctggccgc
     7201 tacgctgtca tcctttacgg gaagggccac agaagcagga aacgtcctca actggacaga
     7261 ccagagcgag acgccggatg tcactttcta tatccagcgt tctgtagatg gcctcaactt
     7321 taccaccatc ggacaagtgg cggcggccag cggcacgggc tccggcggcg gcagtgtcaa
     7381 tcattcattc acggataata gccctacccc taatacccct aatgagtacc gtctcgaatg
     7441 gacggatggc agaggcgatg tcgtctacag caatgtcgtg accctcgtca attccctggc
     7501 cagcggcttg caggacgttt cgccgaatcc tttccgcgac caggtaacgg tgcggttgag
     7561 tcttggccgt acccagccgg tgactatccg gctgctggac agcaagggtc aattgttgcg
     7621 cgaccggcaa tacgccggca tgaagggagc gaactccttc gtgctcgacg ggttatccgc
     7681 gctgccttca tccgtatacc tggttcagat cgttctgacc gatcaggttt ttgtcagaaa
     7741 ggtattcaac aaccggtgat ccgcgggtcc gctgatcgca gcggcgctga cccctaaccg
     7801 aacattaact tttaaatcca agccggcatg aaaatcctat ttcctgtcgc cgccctactg
     7861 ctgttggcag aagctggcgt acacgcgcaa tccagtatgt ctgttacttc cacggaagtg
     7921 aacaatatcc tcttggggtc ctataccccg ggtaactata cggcgtcgac cgtcatcacc
     7981 gaccccgcga ccattgcggc ggggttgctc aacgacattt cggccgactc tctgaaggcg
     8041 gaccttttcg ccctgaacgc ctttcacaac cggaacatgt tctcggacac cacctcgacc
     8101 acgacaggca tcggcgccac ccggcggtgg atctataaca aattcctgca gtacagcgcg
     8161 gtcaatgata accgcctgcg acccgcctat ctaaaattta cctatgcgcc ctccacttat
     8221 ggctgtaccg gtgtgggcag caattcgacc caatataacg tgatggcggt gctgcccggc
     8281 agccagaaga ccaatccctc gctgatcatc atcgaggcgc atatggacag ccgcaattcc
     8341 agcaattgcg acgtcaccgg cagcgccccg ggcatcggtg acaatgcgac cggcaccgcg
     8401 ctggtcatgg agctggcccg tgtgcttagc aaatatacct ttcggtatac catcgtgttt
     8461 tccgtgaata ccggtgagga gcaggggctc atcggcgcgc atgcgctggc agactatctg
     8521 gatagtaaaa atgtgctgat caaggcggtc aataataatg atgtgtccgg aggtgtattt
     8581 tgcgggcata cggcatcggc gcccagctgc cccgcatacg ggaatatcga cagtaccgat
     8641 ctgcgtatct tctcgctcgg tgacgtcaac tcagccaaca aacaatgggc gcgttatatc
     8701 aaactggagt ataaggaaat gttgagcagc cttgtcagcg tcccgaccaa catcgatatc
     8761 atggaagccg acgaccggac gggaagggga ggcgatcacc aggcctttac caaagacggt
     8821 tatacggccg tccggttcac gcaggccaat gaggacggca atgcgagcaa tggccccggc
     8881 tatagcgacc ggcaacacaa tgtccgcgac agtctaggcc attacaacag cgtcacccac
     8941 accatcgact ccatttatgt caatgtcgat tacctggccc gcaatgcgct ggtcaacggc
     9001 aacgcgctcg ccatggcggc gctgggtccc gatacgatca gcatgtccgg cactctttgg
     9061 acagcgggta ccgtgaaggt gaaattctca ccgacatcca gcccgggcta ccgcgtggcc
     9121 gtccgctccg catccaatga ctgggacacc gtatacacgg ttaccggcat gtctaccgcc
     9181 gtgctcacgc tgccatattc cagcaatacc accttttata tcagcgcggc cgcgatggat
     9241 gcgaatggca ccgaaggtca gttctctacg gagtatacgc tttcgacggg gctcattccc
     9301 ctcgacctgc tgcccgatac gactaaaccg gtacccaccg atcccgatta ttacggcatt
     9361 cgcctgatgc ccaaccggcc caatcccttt gacgaatcga cgatgatcgc catcacatcg
     9421 ggtacggata tgtttgctgc ccggacctgt cttcagattg ccgatatcga agggcgggtg
     9481 atcgcgcgta tccccgtgca cctcgttaca ggcctcaacc aggtcatcta ccagcacggg
     9541 ttcggagtat cgggcatgta tatctgttcg ctcgtgatcg atgggttgcc ggtgcaatcc
     9601 actaaaatga tatttcgcgg ctcccattga gcattgaacc taatcctcca gcctcccaag
     9661 ggcggccttc gggccgctct ccccttcggg ggatcgccac ttccatcatt cttcgcctcc
     9721 cgtcccttcg gcggatcccc actttcatca tcaatggtta gtccaatggt aattgctata
     9781 tttaggtcct cttaattgcc tgcatatgaa acatcggatc gtccttcttt ttttcctttt
     9841 ttgttcttcg aaactttttt gccagcatgg aaccctaacc ctatccaacg atttcaaaat
     9901 cgcggagggc cagtataaac atgaaaccgt caccaactcg gtttacttca ataacaattt
     9961 ttataccgtc acgaattccg cccccagcgc gggaaaatgg ctctttacca aattgtacga
    10021 cgtaaaattt tcgcttaccg tgtccgagtt cgacaaggat atgaacaaga tcagggaggt
    10081 cgtactggaa aatggagaca agaatttcgg cccgctgatc cccaagctga tctcctttca
    10141 cgacaggctg tacctgacat attttaaagc tgccgataaa acgtctttca gcgtttatct
    10201 cgccgccctc gatgaaaata acctgacgct tggagagccc aaactgatct gtacgataaa
    10261 acaggataac gtggggctcg ggcagatcga atcggtgatc aatgccgggc tggtgtattt
    10321 cgttatttcg ccggataatg ccaggctttt ggcggtgtgc aaggctgccc ccaacaaggt
    10381 gcaggctgtt cttttggatg acaagctggc gacgataaaa caaagccttg tttcggtgaa
    10441 cctccaggat ttcgagatcc cttcggccgt actgaccaac gacaacaggg cctgcctggt
    10501 gctggattcg aaggacggca cccgcctgct ttgtatcggc ccggatggcg cgaaaactga
    10561 gatacgctat aacgccgcgg ccagctttgc cccctatatc acccgcgcca gactggtccg
    10621 tgacggtaag agcatcatca tctattccac cactatagaa tcgggaggat cggattcgtg
    10681 gtgcaccggg ataatggtgg cgcgactgga ccccgccacc tttaggctat ccaaaccact
    10741 tacctatgcg ctcacgccgg agttcattca ggcgatcagc gaacagggcg gcggtacaaa
    10801 acacaaacac gactatttta tgtccaattt tacgcctcag ctgctggagc tggacaacgg
    10861 ggatgtcgcc attatcggta gccctgaaat gatctccgaa aaaagttcga catccgcgcc
    10921 caatatgaac ggccagaccc atgagatcgc caccaccacg gtgaccgtag ggccggtgat
    10981 cgtcctgttc cccgataaga acgggaagac ctacaacccc gtgatcgtcc cccgggagat
    11041 cattctgtcg aaaagccaga gcagcggatc gggcgccatc cgggtagtag cgtcgccgag
    11101 acagtcggat gtgtccaccg gtttcctggc tacgcagata ggaggcgata tcgtgatcat
    11161 ctacaatgac aatgtgaaaa atctctccaa agatgccggc gaaaagctga cagtctccaa
    11221 gtcgcccggc gatctcgagc tggccgaggc gctcgtgaat aaggacaaaa aactggaata
    11281 ccgtaagctg atcagtgaga ccacgaaagg aagagcgact ttttatttag gcagctcggt
    11341 tcccagtaat tcttcctcca tcgtatttcc catcggaaag gagggccagg gattcaatgc
    11401 cttgaagacg tattatacga attggtgctt tttggagatc aagtagaggc tgacgcccct
    11461 tagaagttgt aaccattaac ccctattatg ccatggaaga gctcctatct ttcctgaacc
    11521 agatcgcctc ttcggaggga tggccgctgt cggccgcatg tcttgcacat ctcggcgaga
    11581 taataaagat ccgcactttg aaacaagaag agcttctgtt atccgcaggg caggtttgta
    11641 agaatatata ttttatccgg agagggttgc tgaagtgctt ttacatcgtg cacgagcgcg
    11701 aggtttccga ctggtttttc ggcgaattgg agacggtcgt ttccatcgac agcttttatg
    11761 accaggtggc cagcgaggac ttcattcagg ctttggaaga ctgtgaactt ttttgcatct
    11821 cgttcgggga attgaattac ctctttcgaa ccttcgttga attcaatgtt gttggccggg
    11881 tgctgtttaa taagtatttg aggatttggc accggcaggc caggaatatc cggatgctga
    11941 cggccgaaga gcggtatcaa ttcctgctca cgacacagcc aaacttaatt cagagagttc
    12001 cggtccggga tctcgcatct tatctcgata tgtcccggga aacgctgagc cggatgcgga
    12061 ggcgtatcca atgacagcct attatagttt cgtttgtttc cgtcggcgtg cgccgacttt
    12121 aggattaagt atcagacggg cgcgagtcgg gttgttacgc atctcattca gccggttttt
    12181 cacgatacgt tttgtctctt cctggatttg ttggaagacc tggtttacgg tttccaggga
    12241 taccttttgg gatggtctgg gattgttgac attgtccggt tgcggttcga gcttgacttg
    12301 gcaatgaaag gctttaagtg gcatgggtcg gtccgggctg tcggccgtaa taccgacgaa
    12361 ttcgccgctc gataacgcgg cgatcttact ggcgggcatg gcaaggtcga gctgcaggct
    12421 ttggctggta gaggtgccgc ggttgttagt gctgatagtt gatcgttcct gtagaatttt
    12481 gccgatctgc tcgctggtga attttgcgga atctccggtg acctggctgg tcagaaggtt
    12541 ggccggcagg ttgacaaggg cttcggcatg ttcgcgaccg tattccgctc gcagttggtc
    12601 gatggtttga atgccgagga caattcccac cttgtgctcg cggcacgtgg caagaatggt
    12661 atccatgttc aggacatgga agctgggaaa ttcatcgata tacacctggc agggtgtgcc
    12721 cccgggacgg ttgatcctct tcagcattcg actgacgaaa aggctgatga cggcgccgta
    12781 aatgtgttgt ctttcgggat ctgagcccag gcagacgatc ttctgagcgt tgggattatt
    12841 gatatccagg ttgagatccg agcccgaaag aaggtaataa agtttcggcg atacgagcga
    12901 gaagagcggg atccgtgcgc tatccaactg cccctgcagc tgatccatga cattgtgttg
    12961 gagggcatcg gtaaacggtt tgaccagcgt agcgatgtcc ggataggacc gtaataccga
    13021 gaatagatgg gcgtagtcgg tagacatgag ttcgaggaga tggggaaggg tgcaatacct
    13081 tccttcttca tattgtctca ggaaccagat attggctgca aaaaagttga cggccgactc
    13141 gacaaagaaa tcgcccttgg atttggcagc tttgcgatta atgccaaaat atagtgttcg
    13201 cgcgcattcc atggcgtcgg atagctcctg caaggtttcc ggagcgagga cattacatcg
    13261 ggtttcgagg gtattgaaat tgaaggaata aaatttcggc ggatttttca gaccggctct
    13321 gacactttcg agttgctgat aagtgaattc cgtgagaccg ggatatttaa ggtcaaagat
    13381 gaaaattgcc atgccttgat cgatagattg ccggatcagt tgtttgaata cgtatcgggt
    13441 cttgcccgaa ccggggctac ccatgacgag ggtaccccgg tagatgtcca ggttgataac
    13501 gctgttccgc cagcggttct gaaaacgata ccgtcccttc aagtgaaaga aattcgggtg
    13561 cgtaaggcgc cgctcttcct gcggaaagct ctcattgtat tgattgaaca gcccgcgttt
    13621 cgccggccag gtcaccaggc ccgtcaacgc cctaacggaa gtatacaaca aaccgagccc
    13681 gcaaacggtt gtcactgcat aaacgatggt tagcatcctc ggatcggcat cgacatacag
    13741 tatgaggcaa ctgccgaaat agaggacgat gccggcgatc aggattacaa taagacggcc
    13801 ggacttgatt tgaggcgggg tttttgcgcc aatgctcgtg ccggccagcg ccaggagggc
    13861 cagtccaagc gacaccgcag gatgacggaa aagacctttt gacgcgatgc cggtcacaag
    13921 acggtccgcg attggacagg tgagcgacca tgtagcgaga ccgcggtggc agaagtagta
    13981 ataatgtagg atgagcagcg cagcgcagca gcaggccgcg aggttccgca attcgcggta
    14041 ttgattgtcg tttgccggtt gcatgtattc ctttttgtag gaatcttgaa ggtagaacca
    14101 cgatcggtac cttccaacac ttaggcgtat tctgtatagg tatcgtttcg gaagtgacaa
    14161 atgtcacata atgtctcaat aactcttttt atgatttaag tgacttttat catttgcctt
    14221 tggtggattt gcatcttagc tttatgaaag ctaaaaataa cgacttatcc tatgcgtgaa
    14281 agggagcggc cttgcttaca catcctggtc gcggatcaac atcggatatt cagggcgggt
    14341 attaggacgc tgctcgccga agcgggttcc tggcaggcct ttgaggtcgg cgaggcggag
    14401 acgacggagg aagccattgc catagtcgca acgggtcgat atcccgtggt gctcatcgag
    14461 tataaccttc cgggaagggg cgggatcaag gcgatggaga tcattcgttc acgatggccg
    14521 gccatcgctg ttattgccgt ggtagaaact gacgacggtg gtcctgctga gcgaatgatc
    14581 gatgccggtg ccaaaggttg cattttgcgc aacgtaggat tggatacatt ggtcggtgcg
    14641 attcggacgg tgatgggcgg acgtcgcttt ttcagcaatg agattgccca gcggttactg
    14701 gagcggccgc ggcgagcgaa gcaggatcca ttggagcggc tgacggcgaa ggaaaagcag
    14761 gtattccact ctattcttgc aggactcaga gacagggaga tagcggcgca aatgggcatt
    14821 gcgaaacgga cggtggataa gcatcggcaa catattcact ataaattggg gacgaaaaat
    14881 gcgctggaac tattgcagac tggcttacga ttgggtctgg taagggggcc ggaatgaatg
    14941 ttccaaaatt ttatctaagc gttttcacta tatttttttt aatatcttga ctacaaaacg
    15001 tcgttgccgg acgcaggcgc catctacaca gttatacaga aaatcgacac gtctatcaac
    15061 gatttccaca cgctggtcat tgaattaggg gactgtatca caatatgaaa atattgcaat
    15121 atgaacgaga tgcgtatgcg ttttcactgc gtctattgtg cctgtctttg gaaaatctaa
    15181 aagttttatt ttacttcgcc ttaatagaaa tcctgccctt accatcttct gcaatttttt
    15241 cttagaccct aaaatcgctt gctatatgag atactacagc ctcctcgagc gcgaaccttt
    15301 gcgcgcacct cctactctgt ttgccgtccg ggcatatttt ctagtttttc tatacaaggg
    15361 ctgagccgcg gtgccctttc ggtcatcact ataagggctt tgtcttcact ttaaaaatta
    15421 ccgtctcgta cggatagggc aggttttgct ttgcccacaa caatcttatg cgctataaat
    15481 ccaaaataag aatgaaacgc tttttctttt tgctcctgat tgtcacgatc gttagcatga
    15541 acaattcgtt cgcccaaact actttctggt ctgtcaccgg caataccggg caacggggcg
    15601 ataccagctt tgtcggaacc atcgacagca accggctggc tttccggaca aagaacaggg
    15661 aacatatgag tatttctccg acgggcacgg tcgccgtggg gactgaaacc gagttggata
    15721 gtttgaccct gaacggtcca atgtatatat gggactcggt gggtatcgat ccattgagta
    15781 tagagagcaa taacgattat gggtcattcg tcaattttac gaatgcaagt acgtactatg
    15841 ctgcgtcaat agggttgagg ttctataatt ccgccggtat cgtcagtcaa atgtttgtcg
    15901 gcagcccttc cagtatctac gccccgaacg gttttggctt ccaggatctt tccggaggag
    15961 gttttgactt ttcttcccgc atcgccggaa gcttttttag ctggggcagc gattttaagg
    16021 cggcgggtgg ttcgaaaatg attttctaca ccacacccgg acatttgatc atcggctcga
    16081 caaccgatag cgggaacagc aagttgcagg tgaggggaaa tgcctggatg caaggattga
    16141 cgcttccaac gggtgctgct tctggatatg tgctgaccag cgacacggcg ggtaacgcga
    16201 gctggcagcc gggctcttcg gggcgatggg cttttgccaa tggctcagtg ttcgattcct
    16261 cggataatat tgccatcggc acgagcaatc ctcagggata taaactcgcg gtcaatggta
    16321 ccgccatgtt cacgaaaata aaagtacgac ccttgtcgag ctggccggac tatgtcttca
    16381 ggaaggggta tgtattacca gggttggagg aactggaagc gtatgtcaac cagtatcatc
    16441 acctgccggg gataacatcg gaagatgagg tgaagcaaga cggtatcgat ctgggcgagc
    16501 agcatacggc gctgttgaaa aaagtggagg agcttacgct ttacctgctc aatgaaaatc
    16561 gctccttgaa ggatcagatt aaagagctct ccgaacagaa aagccaactg gccgagcaaa
    16621 atgctcgcct gcaagcgcag caaaaagaga tcgatgatct caaggcgatg atccaggcca
    16681 ataccaaaca ttaatctggc ttttatgaaa aagatactgg tagcctgtgc agtcggactc
    16741 agtttgaaca taggtatctc gcgggcacag tcggtcgact cggcggccgg aaaggtagta
    16801 agctttccat ccaggctttt ttcgcggata cagtcgcgta cggcccggct caacaatcag
    16861 ttgacaagcc aaactcagaa atatctgcaa aaaatggccc ggagggaaca gcaacttcaa
    16921 cggcggttgg catctgtcga tccggcggcg gcgcagcggc tgttcgcggg cagcgcccag
    16981 cgctatgcgg tattggagca gcagatcaag gcggatacgg gcggtagcgg ccgttcattc
    17041 agcggggtct atatgcccta tctcgattct ttacagggtt ctctagcatt ccttcagaaa
    17101 aacccgcagc tgctacccaa tccgggcgtc ggacagttga cacaattaca ggcggcaggt
    17161 ggccaggtaa aatcatacga ggcaaaatta atggatgcag accaggtcaa ggcgtatatc
    17221 cagcaacgga aacagatgat cgggcaatat atcacgcaac atgccaacct gcagggattg
    17281 ctggggaagt cttatgccgg tatgaaccag gacgtttatt actactctca acaattgcgg
    17341 gcatataagg aaatgtggaa caatcccgat cagctattgc aaaaggcact cgtggtgctg
    17401 aacaggttgc ctacattcca atcgttcatg aaaggtaact cgatgctcgg gactcttttc
    17461 aatctgccgg gcaattatgg ttcgccacag gctatcagcg ggctacagac caaggatcag
    17521 gttgcacaac aaatacaggg gcacgtgtcg gcaggaggca cgggtggagc ggccgcgtta
    17581 caatcaaatc tgcaattggc gcagtcgcag ctggctggct ataaaagcaa actcagccag
    17641 ctgggttcag gcaatgggga tatggatatg ccgaatttca aacccaatga tcagaagacg
    17701 aagacttttt ggaagaggtt ggaatatggc gccgactttc agacgacgcg caacaactat
    17761 tactttccga tggtgactga ttttgggttg tcgctgggtt ataagctggg gcataacaat
    17821 attatcggcg tgggggctag ctacaaacta ggttggggga atggaataca gcatatcgcc
    17881 tttaccaatg aaggggtggg attgcggtct tttttgcaga tcgcggtcaa aggcagcttc
    17941 agcgcgacag gtgggtttga gtataattat actactccat tcacttctta ccagcaacta
    18001 aagcaaattc aatattggac caagagcggg ctcatcggcg cgacgaagac ggtgtcgttg
    18061 aagagcaggg ttttcaagaa gacgtctctg agcctgcttt gggacttttt gagctatcag
    18121 caggttccac ggacgcagcc gattttgttc aggattgggt ataatttcta aaagacgtaa
    18181 ttcaataaat atgcgaaggg ttattttgtt cttgcttttt tccggcgtca gtttatttgg
    18241 ttatacacag tccacgactg accctacaca gggtaatatc aatgttgtta cacctagaac
    18301 tccggagtcg gcaggctttg aaaagttcgg caaatacgat gtgaatgaat tcactggtac
    18361 ggcgaatatt tccattccgc tttatacact gtccagcaga catttggctg tgccaattac
    18421 gctttcttat catcccaccg gtatcagggt caatgaagaa gccagttggg tcggcctcgg
    18481 atgggatctg attgccggag gaagaataac ggtagaaacc aaaggaagtg tggactttgc
    18541 cacagggtca gtaggtggga atgggccttc cagcactgcc aacgaaatgc aaaggatatt
    18601 caagcatttt gatggaggag gtgaacttgg catcgcgact tttccaacat tttttgaaac
    18661 cgatgtgggt agttccggac aactcgcttc ggatgtcgat tcaaacctgg atatcacttc
    18721 aggcgtccag gacatggatg aatttggcac gggtgaaccc gatatatttc gagcaaactt
    18781 catggggcac actttcaatt tctattttga taagataacc
//
LOCUS       DASYSV010000108         7850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_21775, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000108 DASYSV010000000
VERSION     DASYSV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21775"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1918
                     /locus_tag="VGQ51_03475"
     CDS             <1..1918
                     /locus_tag="VGQ51_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035649.1"
                     /translation="IDNDDHVLISSAISENTPRVVKTMTVAEVQSSLQLGQQETALSK
                     DTAVVAPKVYFRMEPTALVLIDGDPVLVRNDRWGLDFVKNTRNVIVQDKDGKFYLYGG
                     GYWYVAPSATGPYVLEGERVSRTLRKIERDLAKAARKSDVPFTESEMMLNHIIVSTVP
                     AVLVQSDGTPKGAPMPYSSLFYVKNSDNAIFYDTVSKHFYVQGGGNWFQGDSLYGDNN
                     WHLISKVDLPAELLVSLSGWEWNGEGPVAGRMAARKDATVNAVMAEQVPQTARVDRTI
                     TTSVDYDGEPRFAPIPGTALEYATNTCSIVLKYNDAYYALDNGIWFIASSPLGAWRVS
                     DVRPEGVELIARKYRVYRAKFVFIYQTTPTAVYEGYLPGYEEAPVDGCGMAVAYDQDW
                     SNMAWSYDLDYVFDWGLGFLDGYYRYDPWNYYYGYMVFGGRHPEWRWKAHGPATKGGG
                     GAPGSGSGGVYASRGVGPHGRPPVGAAPGSSSTRVAGSGRSAGLPPHPPGGWGQRTQN
                     IGYSGVVVATPHQPGMPRVSGGPSGNVGGLHGLPSGGNAARVGNTGGQVSRGYSGGGY
                     SGGGRPSGGYSGGGGGAVRSSNSGGGSGGGGHVSSGGGGGGHVSSGGGGGNSGGGGGG
                     HSSPPSGGGASTHH"
     gene            complement(1915..2985)
                     /locus_tag="VGQ51_03480"
     CDS             complement(1915..2985)
                     /locus_tag="VGQ51_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEV9035650.1"
                     /translation="MNNIPHIFRSGPATLRHLILAAALFILAPCAKAQDPGFSQFFAS
                     PMTLNPALTGKFSGVLRVAGNYRNQWPAINNAFITNTISIDAPILVKRLPENDTWGIG
                     LMAMTDKTASGILNDNYFAVSTAYHLALDPDGYHSIGVGFQGTYGNQSLDGTRLTFED
                     GLQLDGTWLRSPSEAINSEFVSVHYFDMGLGALYNASTNGNNNYYFGFSAYHLNHPKV
                     SFLGVDTINVPTRLTLHGGGFFPIPGSPSTVYVSGLVNDQAGAREYVLGGAWQIAAST
                     DETNPVNFYAGMWGRFTNNTTDALIPYVGLDYSNFSLGVTYDVNISSLQTASQNRGGI
                     EVSLIYIQKASNGHHFVPCPRF"
     gene            complement(3096..3746)
                     /gene="upp"
                     /locus_tag="VGQ51_03485"
     CDS             complement(3096..3746)
                     /gene="upp"
                     /locus_tag="VGQ51_03485"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV9035651.1"
                     /translation="MVVNLSEQFSILGDWISEIRDEDVQKDRLRFRHNLVRIGEVAAY
                     EISKTLPYIEKEVETPLGTTIVKVLKEQPVITTILRAGLPMHQGLLNFFDKADNGFIS
                     AYRKHQRDGSFEISLEYVSCPELENRVVIISDPMLATGASLVKTIQYLREEGHPSEIH
                     VVVAIACTVGIEFVLRSEPAAKIWCGAIDDELTAKGYIVPGLGDAGDLAFGNKAQM"
     gene            complement(3802..4785)
                     /locus_tag="VGQ51_03490"
     CDS             complement(3802..4785)
                     /locus_tag="VGQ51_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV9035652.1"
                     /translation="MNTSISRPSRLLLILAFTAVYIIWGTTYLAMRFGMLGIPPFMLC
                     LFRYALVAVILLAWMGIKGLSFPSAKNTKILSASGLLMLVGGTGLVVTAEQYINSGAT
                     ATVIATEPLMFLILDRTSRKSYSFATVAGIVLGFAGIFLFSKFTSNHGDALSGAGSGD
                     VWKGTLMVLVSAAFWVLGALQSRRQTNADHSPVASAAVQHIVAALGCLPIVLARGEFA
                     TFHLAQVPAAAWLGLAYLVIMGSLVAFMAYMWLMKVQPPAIVSTHTYVNPIVAVIAGW
                     ILAGETINAPQVLALAMVLIGVLLVQSPRLQRIRLPRPRRTPYRPHSQPWS"
     gene            4870..6417
                     /locus_tag="VGQ51_03495"
     CDS             4870..6417
                     /locus_tag="VGQ51_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV9035653.1"
                     /translation="MIIIQNLVRIDKDRDEPVYLQIANGIAGIIQQGILKPGTALPSS
                     RKLAEMYDIHRKTVTAAFDELQAMGWAESLPRKGFFVASELPVVKPRAVSAARAAAAG
                     LSRGAGPGTGIVGAVAALGRPGIGYPSATAFAIENRPYGYDQPFVARRERLAFNDGFP
                     DTRLAPVELLVREYRRFSNYRFTAKFLSYGPAQGSENLRNEMARFLNVTRGLSIGPEN
                     ILITKGVQLAIYLVSRLLLKKGDEVIVSEPGWTGTSEVFRAAGASLNFVGVDDDGMDI
                     DAVERICRVKKVRMVFVIPHHHMPTTTTLCAARRQKLLELARRYEFAVLEDDYDFDFQ
                     YSSSPILPLASVDTSGHVIYVGSFCKIIAPAIRIGFLVAPVNLIAQATRLRKMVDRQG
                     EQLMEEAMANLLRNGDIDRHLKKAGKIYHERRDRLSGLLASELSGRVEFREPDGGFAA
                     WVRFVGDRPVREISVAASRLGLAMSDGEDYWHVKPPRVNGVRLGFASLNEKEMEEAVG
                     ILRRVVG"
     gene            complement(6414..6833)
                     /locus_tag="VGQ51_03500"
     CDS             complement(6414..6833)
                     /locus_tag="VGQ51_03500"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HEV9035654.1"
                     /translation="MNHVALTSSESKIRLTAFFVLLFILTYLITRNPWLILFLLIDFS
                     FRSTPELAKYSPLANLSGLISKTFRLANKPVYMPPKRFAARIGLLFIITIIIAQNSKP
                     GTLILGSTIALFAALESLAGFCTACWIYSAFMRLRSH"
     gene            7196..>7850
                     /locus_tag="VGQ51_03505"
     CDS             7196..>7850
                     /locus_tag="VGQ51_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEV9035655.1"
                     /translation="MMNSKIWLGWVLVGAMGCHEAGRRGASAGVGTATGKAQAAGVAR
                     EAAAMARVAAAKKSAICCEKNIPARFAVAAVLPVRGDTVAGARDIAGRDIAGHATHEG
                     MIWIPGGTFRMGADNDQAAADEYPKHLVTVSGFWMDRSEVTNVEFADFVRATGYVTTA
                     ERAPDWNELKKQLPPGTPKPDASLLVPASLVFTPPDYPVELGDYSQWWSWVKGADWRH
                     "
BASE COUNT         1608 a         1669 c         2868 g         1705 t
ORIGIN      
        1 tatcgacaat gacgatcatg tgcttatctc atcggccatt tcggagaata ctccgcgcgt
       61 ggtaaagacc atgaccgtcg cggaagtgca gtcgtcgctg cagctgggac agcaggagac
      121 cgccttgtcg aaggatacgg ctgtcgtcgc gccgaaggta tatttccgga tggagcccac
      181 ggcgctggtg cttatcgacg gggacccggt gctggtcagg aacgaccgct ggggactcga
      241 cttcgtgaag aatacccgga atgtgatcgt tcaggataag gacggaaaat tctatttata
      301 tggcgggggt tactggtatg tcgcgccttc ggcgacggga ccctatgtcc tcgagggtga
      361 gcgcgtcagc cgcacgctcc gcaagataga acgcgatctt gcaaaggcgg cgcgaaagag
      421 cgacgtgcct tttacggagt cggagatgat gctgaaccat atcattgtga gcacggtgcc
      481 tgcggtgctg gtccagagcg acgggacccc caagggtgca cccatgcctt attcttccct
      541 gttctatgtc aaaaattcgg ataacgccat tttttacgat acggtgtcga agcatttcta
      601 tgtgcagggg ggtggtaact ggttccaggg cgactcgctg tacggcgaca acaactggca
      661 tttgatcagc aaggtcgatc tgccggcgga acttttggtt tcgctgtccg gatgggaatg
      721 gaatggggaa gggcctgtcg ccgggcgtat ggcggcacgg aaggatgcca ccgtaaatgc
      781 ggtgatggcg gagcaggtcc ctcagaccgc aagagtcgac aggacgatca cgacttcggt
      841 ggactatgac ggtgagccgc ggtttgcgcc gatacctggg acggccctgg aatatgcgac
      901 gaatacctgt tcgatcgttt tgaaatataa cgatgcctac tatgcgctgg ataacggcat
      961 ctggttcatt gcgtccagcc cattgggcgc ctggcgtgtc agcgacgttc ggccggaagg
     1021 tgtggaactg atcgcgcgta agtaccgggt ctaccgggct aaattcgtct tcatttatca
     1081 gacgacgccg acggcggtat atgaaggtta tttgccggga tatgaagagg ccccggtgga
     1141 tgggtgcggg atggccgtag cgtatgacca ggactggtcg aatatggcct ggagctatga
     1201 cctcgattat gttttcgact ggggcctcgg gttcctcgat ggatattatc ggtatgatcc
     1261 atggaattat tactatggat atatggtctt tgggggccgg cacccggaat ggagatggaa
     1321 ggcgcatggt cctgcgacga agggcggggg cggtgctccg ggtagtggtt ccggcggcgt
     1381 atatgcgagc cggggtgtgg gtcctcatgg gaggccgccg gtcggtgcgg cgccgggttc
     1441 ttcttcgacg cgggttgcgg ggtccgggcg gagtgccgga ttgccgccgc atcctccggg
     1501 aggatgggga cagcgtacgc agaatatcgg gtattccggt gtggttgtgg ctacgcctca
     1561 tcagcctggg atgccgcgtg tttcaggtgg tccttcgggg aatgtcggcg ggttacacgg
     1621 acttccgtcg ggaggtaatg cggcgcgggt tgggaatacg ggtggacagg tttcgcgtgg
     1681 ctattcgggt ggagggtata gtggaggggg gcgcccttcg ggcgggtata gtgggggtgg
     1741 tggtggtgct gtgcgcagta gtaattcggg aggtgggtct ggtgggggag gacatgtgag
     1801 tagtggcggt ggtggaggcg ggcatgtgag ctcaggtggc ggaggtggga attccggtgg
     1861 aggtggtgga gggcattcga gtccaccttc tggtggtggc gcgagtacgc atcattaaaa
     1921 tctcgggcag ggcacgaagt ggtgaccgtt ggaagctttt tggatataga tgagcgagac
     1981 ctcgatgccg ccacggtttt gagaggccgt ttgcagggaa gatatattga cgtcataggt
     2041 gacgccgaga ctaaaattac tataatccag ccccacgtat gggatgaggg cgtcggtcgt
     2101 attattcgtg aaccggcccc acatccccgc gtagaaattg acggggttgg tctcgtcggt
     2161 gctggccgcg atctgccagg ctcctcccaa tacatattcg cgcgcacccg cctggtcgtt
     2221 gaccagaccg ctgacataaa cggtgctggg tgaaccgggg ataggaaaga atccgccgcc
     2281 atgaagggtt aggcgggtgg gaacgttgat cgtgtcgacg cctaagaagg acaccttcgg
     2341 gtgattgagg tggtaggcgc tgaagccgaa ataatagttg ttattgccat tggtcgatgc
     2401 gttatacagc gcaccgagtc ccatatcgaa ataatgcacg ctgacgaatt ccgagtttat
     2461 cgcctcactg ggcgagcgga gccaggtgcc gtcgagctgg agaccgtctt cgaaggtaag
     2521 gcgggtgccg tcgaggcttt ggttgccgta ggtgccctgg aagccgacgc cgatgctatg
     2581 atagccgtcc gggtcgagcg cgaggtgata ggccgtggaa acagcgaaat agttgtcgtt
     2641 gaggataccg ctggccgtct tgtccgtcat ggccatcagc ccgatgcccc aggtgtcgtt
     2701 ctcgggaagg cgtttgacga ggatgggagc gtcaatggat atggtgttgg tgatgaatgc
     2761 gttgttgatg gccggccatt ggttgcggta gttgccggcg acgcgaagga ctccgctgaa
     2821 tttgccggtg agggccgggt tgagggtcat gggggaggcg aagaattggg agaagccggg
     2881 gtcctgggct ttggcgcagg gggccagaat aaataaagct gcggcaagga tgaggtgccg
     2941 gagggtggcg gggccggatc ggaagatgtg aggaatgttg ttcataaaca gttgcgtaaa
     3001 ggtatagacg gataaatgga tcgcgcggtt ggcttttgga ggggatatgg tggggaaaat
     3061 tagcaaaaaa gggtgaaacc gcaaagcggc gggggttaca tttgggcctt gttgccgaag
     3121 gcgaggtcgc cggcgtcgcc gagaccggga acgatataac ctttggcggt gagctcgtcg
     3181 tcgatggcgc cgcaccagat ctttgccgcg ggctcgctac gcagcacgaa ttcgatgccg
     3241 acggtgcagg cgatggccac taccacgtgg atctcggagg ggtggccttc ttcgcgaaga
     3301 tattggatgg tcttgacgag gcttgcgcca gtggccagca tggggtcgga gatgatgacg
     3361 accctgttct ccagttcggg gcaggagaca tattcgaggc tgatctcgaa gcttccgtcg
     3421 cgttggtgtt tgcgataggc ggagatgaag ccgttgtcgg ctttgtcgaa gaaattgagg
     3481 agaccctggt gcatggggag gccggcgcgg aggatggtgg tgatgacggg ttgttctttg
     3541 aggactttga cgatggtggt gccgaggggg gtttcgactt ccttttctat gtatgggagg
     3601 gtcttactga tctcataggc ggcgacttcg ccgatgcgca cgaggttatg acggaaccgg
     3661 aggcgatcct tctgcacgtc ttcatcgcgg atttcgctga tccaatctcc caggatggag
     3721 aattgttcac tcagattgac gaccataatg atggggtttg cgggcacaaa cctacaataa
     3781 tattgggaga acggcaatgg gtcacgacca cggttggctg tgaggacggt agggcgtgcg
     3841 acggggccgc gggagcctga tccgttggag acgcggggac tggaccagca ggacacctat
     3901 caggaccatc gcgagggcca ggacttgcgg ggcatttatc gtctcgccgg cgaggatcca
     3961 gcccgcgatg acggcgacga tagggttgac atacgtgtgg gtgctgacga tggcaggcgg
     4021 ctggactttc atcagccaca tataggccat gaaggcgacg agcgagccca tgatgacaag
     4081 ataagcgaga ccgagccatg ctgcggccgg gacctgcgcg agatgaaaag tggcgaattc
     4141 gcctctggcc aggacgatgg ggagacagcc gagtgctgcg acgatatgct ggacggctgc
     4201 gctggcgact ggggagtggt cggcgtttgt ctgtcgcctt gattgaagcg cgccgagcac
     4261 ccagaatgcg gcgcttacca ggaccatcag ggttcccttc catacatcgc cgctgccggc
     4321 gccggataat gcgtcaccat ggttgctggt gaatttcgag aaaaggaaga tgccggcgaa
     4381 tccgaggacg atgccagcga cggtggcgaa ggaataagac ttgcggctgg tgcgatcgag
     4441 tatcaggaac atcagtggct cggtggcgat gacggtggct gtggcgccgg agttgatgta
     4501 ttgttccgct gtgacgacca ggcccgttcc cccgacgagc atcagcagcc cgctggcgct
     4561 gaggatcttc gtattttttg cggacgggaa ggaaagacct ttgatcccca tccaggcgag
     4621 caggatgacg gctaccaggg cataccggaa aagacagagc atgaaaggcg gaatgccgag
     4681 cattccaaaa cgcatagcca gataagtggt tccccagatg atatatacag ccgtgaaggc
     4741 gaggatgaga agaagccgcg aggggcggga aatggacgtg ttcataatat cgaaattgca
     4801 ggtgtaaaat tactccacca aaaaggtata tttatagtcc agaatctata aatgaggtag
     4861 tccaagaaca tgatcatcat tcaaaacctg gtgcgtatcg ataaggatag ggacgaacca
     4921 gtgtatttac agattgcgaa cggaattgcg gggatcatcc agcaaggcat tttgaaaccg
     4981 ggcacggcgt tgccgagcag caggaagctg gcggagatgt atgacattca ccggaagacg
     5041 gttacagcag cctttgacga gctgcaggcg atggggtggg ctgagtcgtt gccgagaaag
     5101 ggtttttttg tggcgtcgga gttaccggtg gtgaagccgc gggcggtgtc ggcggccagg
     5161 gctgctgcgg cggggctttc ccggggtgcg ggaccgggca cgggtatcgt gggggcggtg
     5221 gctgcattgg gacgaccggg gataggctat ccgtctgcaa ccgcatttgc aatcgagaat
     5281 cggccatatg gatacgatca gccatttgtg gcccgccgcg agcggttagc ctttaacgat
     5341 gggttcccgg acacgcggct cgcgccggtg gagttgctgg tccgcgagta ccggcgattt
     5401 tcgaattacc ggtttaccgc taagttttta tcttatggtc cggcgcaggg gtcggaaaat
     5461 ctgcgcaatg aaatggcgcg ttttctcaat gtgaccaggg gtttgtcgat aggaccggag
     5521 aatatcctga tcacgaaggg cgtccagctg gcgatatatc ttgtgagccg gttgttattg
     5581 aaaaaaggcg acgaggtgat cgtcagtgaa cccggatgga cggggacgag cgaggttttc
     5641 cgggcggcgg gagcttcgtt gaactttgtc ggtgtcgacg atgacgggat ggatatcgat
     5701 gccgtggaaa ggatctgtcg ggtcaagaag gtgcggatgg tctttgtcat cccgcatcat
     5761 catatgccga cgacgacgac gttgtgcgct gcgcgccgcc agaagttgct ggagctggcg
     5821 cggaggtatg agtttgccgt gctggaagac gattatgatt tcgattttca gtattcatcg
     5881 agtccgatat tgccgttggc gagcgtggat acgagcggac atgtgatcta tgtgggatcg
     5941 ttttgcaaga tcatcgcgcc cgcgatacgg atcgggtttt tggtggcgcc ggtgaatctg
     6001 atcgcgcagg cgacgcgttt gcggaagatg gtggaccggc agggggagca gttgatggag
     6061 gaggcgatgg cgaatttgct gcgcaatggg gatattgaca gacatttgaa aaaggcgggg
     6121 aagatatatc atgagagaag ggatcggttg agtgggttgt tagcttcgga gttatccggg
     6181 cgggtggaat ttcgggaacc ggatggaggg tttgcagcgt gggttcgctt cgtgggggat
     6241 cgtccggtgc gggagatatc ggtggcggct tcccgtttgg ggcttgcgat gtcggatgga
     6301 gaggattatt ggcatgtgaa gcctccgagg gttaatgggg tgaggctggg gtttgcttct
     6361 ttgaatgaga aggagatgga ggaggcggtg gggatattga gacgtgtggt gggttaatga
     6421 ctgcgcaaac gcatgaaggc gctatagatc cagcaggcgg tgcagaatcc ggcgagcgat
     6481 tcgagggcgg cgaagagggc gatggtggag ccgaggatga gtgtgccggg cttgctgttt
     6541 tgcgcgatga tgatagtgat gatgaatagc aggccgatgc gggcggcgaa acgtttgggc
     6601 ggcatgtaga cgggtttgtt tgccaggcgg aaggttttcg atatcaggcc gctgagattt
     6661 gcgagggggc tgtattttgc gagttccggg gtggagcgga aggagaagtc aattaataag
     6721 aataggatga gccaggggtt tcgtgtgatg aggtaggtga ggataaagag gagcacgaag
     6781 aaggcggtga gacggatctt tgattcgctg gaggtgagtg cgacgtggtt catggggtaa
     6841 aggtaaagaa agattttgaa tagtcgagtt gcgtggtgga ctaatggagg gcgggcggca
     6901 gcggtgcggg tggcgggatg ggacggtcgc ggatttggcg agggtttggt gcgtcgggcc
     6961 agtgggccct ggatgagagc gaggggctgc ggcttctggg tgagggtgga gggcggcggc
     7021 ttctgggtga gggtggcggg cagcggcccg cggatgagcg cgggggaatg tatttatttt
     7081 tgcggggatt tttggtacta aagttaactt gctgaaagtc aagaatataa ttttggttcc
     7141 aaaaaacggg gtgaaataaa ggaaatttgg cgctgtggac ctctaaatga aaattatgat
     7201 gaattcgaaa atatggttgg gttgggtgct ggttggagcg atgggctgtc atgaggcggg
     7261 gcggaggggt gcgtcggctg gtgtcgggac ggctacgggg aaggctcagg cggcgggcgt
     7321 ggcgcgggag gcggcagcaa tggcgcgggt ggcggcggcg aaaaagagtg caatttgctg
     7381 tgagaagaat attccggcgc ggtttgcggt ggcggcggta ttgccggtgc gcggagatac
     7441 agtggcggga gcgcgtgata ttgcggggcg tgatattgct gggcatgcga cgcatgaagg
     7501 gatgatctgg atcccgggag ggacttttcg catgggcgcc gataatgatc aggcggctgc
     7561 cgatgaatac cctaaacacc tggtgacggt atcggggttc tggatggata ggtcggaggt
     7621 aaccaatgtg gaatttgcgg attttgtacg cgcgacgggg tacgtgacga ctgccgaaag
     7681 ggcgccggac tggaatgaat tgaaaaaaca gctgccgccg ggtacgccaa agccggacgc
     7741 ttcgctgcta gtgcccgcct cgctggtctt cacgccgccc gattatccgg tggaactcgg
     7801 cgattatagc cagtggtggt cctgggtgaa gggggccgat tggcggcatc
//
LOCUS       DASYSV010000109        10763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_535473, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000109 DASYSV010000000
VERSION     DASYSV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535473"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..1056
                     /locus_tag="VGQ51_03510"
     CDS             373..1056
                     /locus_tag="VGQ51_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9035656.1"
                     /translation="MKSIVTFSQTLRITFFAIILFAVAGQQANAQQTQSSNAVVTPAL
                     LLSFDGQVENSCANLTWVMENETNCKMFVIERAGEKGGYDSINVVEGMNNSNQTTYTF
                     EDENMVIGNNYYRLRQVDRDGVVRYSKIVTLYKNAPAVAANTIGIYPNPATSTINYTV
                     ASTTSQQVIVQVFDISGVVLLTTQQQLYAGNNQQTVGLSGLKNGNYFLRVTNREGSTQ
                     YVQPFVKIM"
     gene            complement(1120..3837)
                     /locus_tag="VGQ51_03515"
     CDS             complement(1120..3837)
                     /locus_tag="VGQ51_03515"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV9035657.1"
                     /translation="MTTLKLDFYLRFYTHPGQSILLSGNLPELGNGEIAAAIPLEYVN
                     GEFWHRSVVLGDIPSSPVQYHYILKNIDGTLTEEWGDDKFIETPAATVEELQVIDSWN
                     YAGEFENVFYTSPFRQVLLPRHKGKKQKKKTGTTHVFRVKAPLLAADEVVGLLGNAGA
                     LHDWGADEADPLLLSPDGDWWTLALSIPPEAFPIEYKYAVYHKKDRRLIGFEDGPNRR
                     LPGDARPNKISILHDGFVHMPNRGWKGAGVSVPVFSLRSKRSFGVGEFTDLKALIDWA
                     VKCGLRLVQILPVQDTTASHTWQDSYPYAAISAFALHPLYLNLEQCAGKKYAQLIKPL
                     HKKQKELNALPEVDYEQVMKIKLLAIKELYELQKEEFKDVPDFQTFFRQHRNWLVPYA
                     AFCYLRDKHGTPDFTRWKMHNNYDEDAILRYTAPGTRHYDSIALHYFTQYHLHLQLRE
                     AVDYAHENGVILKGDIPIGIFRYSCDAWVEPRLYHMEMQAGAPPDNFAVKGQNWGFPT
                     YNWEEMRQDGYSWWKRRFAHMNNYFDGFRIDHILGFFRIWSIPMDVVEGILGRFVPAI
                     PVYRVEFDQRNIWFDHDRYTRPFINEAVLWELFGNESDHIKDNFLHPTGNGFYVLREP
                     FDTQRKIEQWFGHQPPGSCNEKIRQGLYDLVSNVILLGIEGSEGQEYHFRISMEHTSS
                     FRYLEWHTQQQLKDLYVNYFYSRQDDFWQRQAMRKLPALKRATNMLVFGEDLGMVPRS
                     VPDVMRQLGILSLEVQRMPKNPQKEFSHPAHAPYLSVVTPSTHDMSTIRGWWEEDRTI
                     TQRFFNRELGQWGEAPVFCEPWINKAIVLQHLHSPAQWAIFQLQDIMGISDEFRRENP
                     HDERINVPANPHYYWRYRMHLTLEELLKEKTFNAEFRGEVENCGRWFPE"
     gene            4011..4166
                     /locus_tag="VGQ51_03520"
     CDS             4011..4166
                     /locus_tag="VGQ51_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035658.1"
                     /translation="MEQQKKNTGAYWILFFVSAAALIWAVATHFSYLTLILPFVCTSF
                     VKAMDIM"
     gene            4166..5974
                     /locus_tag="VGQ51_03525"
     CDS             4166..5974
                     /locus_tag="VGQ51_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035659.1"
                     /translation="MSLHSYYQGQVDACRMEIRRLQNKLNSLAILRVLVFAWFAGSVY
                     TLIQGLSAGWLVSAILAAAGFIGCVNIYLRWKDKRRLQEKLRYVCENELAQLGGAENR
                     FPDGGSWLDSANYLDDLDIFGPRSLFHVLNRTTTAPGASDLAARLRQPFLMSGTIQQQ
                     QEAIRVLTPQAVIRQLLTATGMVSGEDAQRAGQSGGHNASENKNLDDWLDTPPRLVNR
                     PWLWLVIAALSLFNLYGLYLFFDNGNYTLIIAGIIISRAITGAWSRYINTQHRLIGHQ
                     HALLDQYAGILSVFNQTATTGSHLLQQVQESTEKASSAMRRLSRLSSYFDQRLNLLVN
                     LILNTFFFYDLLCMIALERWKTANRAGFPAWIDAVGTVEALNSLAGFAYNHPEYIYPS
                     PVAGPLLIEATGLAHPMIPATRRIANDIAIGRDQRLILVTGSNMSGKTTFLRTVGVNL
                     LMAQCGLPVCATTFTFTPMQLLTSLRITDSLQEQTSYFMAELNKLQMIIHRLRAGEPA
                     LVLIDEILRGTNSEDKTYGSEQFIRRLLQYPCLSFFATHDLTLGQLEGEMPGAISNYC
                     FESVIEGEELHFDYRLRRGVARNRNASFLMRRMDII"
     gene            complement(6009..7919)
                     /gene="dnaK"
                     /locus_tag="VGQ51_03530"
     CDS             complement(6009..7919)
                     /gene="dnaK"
                     /locus_tag="VGQ51_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV9035660.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVIANDEGHRTTPSIVGFLK
                     NGERKVGDPAKRQAITNPVNTIMSVKRFMGRRYDEVTNEITHWSYKVVKGDNNTCRID
                     IDGRLYTPQEISAMILQKMKKTAEDYLGQEVTEAVITVPAYFNDAQRQATKEAGEIAG
                     LTVRRIVNEPTAAALAYGLDKQGKDEKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDKVVMDWLADEFRKDEAVDLRKDPMALQRLKEAAEKAKIELSSSSETEIN
                     LPYITAVDGVPKHLVKKLTRSKFEQLADSLFERCLKPCEQALKDAGISASQIDEVILV
                     GGSTRIPKVQDLVEKFFGKKPNKGVNPDEVVAVGAAIQGAVLTGEVKDVLLLDVTPLS
                     LGIETMGGVMTRLIDSNTTIPTKKSETFSTASDNQPGVQIHVLQGERPMASQNKSLGM
                     FNLNDIPPAPRGVPQIEVIFDIDANGILHVTAKDKGTGKEQKIRIEAGSGLSKEEVEK
                     MKAEARANESSDKATREKIDKLNQADSRIFQTEKQLKEFGDKLPADKKAPIETALSKL
                     KEAHKSEDVAAIDAAITALDNAWTVASEEMYKASQQQGGQPGGGPNGPDGGGQANTGS
                     ENVTDAEFEEVK"
     gene            complement(8048..9436)
                     /locus_tag="VGQ51_03535"
     CDS             complement(8048..9436)
                     /locus_tag="VGQ51_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV9035661.1"
                     /translation="MNKKTPMVTVVIPALNEEKTIRQAIRQAKRNQLVDEIIVVDDMS
                     TDDTIAEARNEEVKVITSTHVGKGDSMREGLLMAKNDIVVFMEADIPDYEVDIVGMLT
                     APLIRDEADFVKSYFERSGGRMAEILAKPLLEFFFPHLLKFKQPLSGMIAGKKSILQK
                     VEFDNDYGVDIGLLIDMHQVQARIAEVCIGEVGNDRQPLHVLSRMAKQVANAIFKRVN
                     IFDSRRQQEDAPPAMREQVEFALKELVRAPKKMIVFDMDHTLLQGSFIQAAAKQFGFT
                     RELADILGQQKSNYIKTQKIARLLEGRTFAELIDVVESIPLIGDAVQVIRELQVRGYV
                     CGIISDSYHAIANHIKNLLGLDFTLGNELEFRKSVATGEVKIPSQLLRDRFSQCDHDH
                     CKSNMFQYILHRFGISLADSVAVGDGENDICMVKMAGTGISFNSITPILDEVADYVFR
                     GNSLKPVLEVVR"
     gene            9555..10004
                     /locus_tag="VGQ51_03540"
     CDS             9555..10004
                     /locus_tag="VGQ51_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9035662.1"
                     /translation="MPIAPDIYRRIVSAKLYIDGNFRERLDLDRLAREACLSRYHFHR
                     LFTRIYRVTPHQYLTRKRIEAAQLGLAGQARTVTEVCNDVGFESIGSFSSLFKKEIGH
                     APASYRSLTHGRNRQVKDSPRSFIPHCFIETLTTKSNSQEAVSPAKS"
     gene            10028..10441
                     /locus_tag="VGQ51_03545"
     CDS             10028..10441
                     /locus_tag="VGQ51_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9035663.1"
                     /translation="MITRVSHVTFFVTDQQKAHEFYVGILGFRVNTDATMENGFRWLT
                     VSPPDQPDLEIALIAPLNGGMGYDEETTQAFRVLLEKGALGAGVMDTPDCRATYEELR
                     SKGVGFKGEPKDQFYGTEAILFDGIGNWFSMTQRK"
     gene            complement(10468..>10763)
                     /locus_tag="VGQ51_03550"
     CDS             complement(10468..>10763)
                     /locus_tag="VGQ51_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV9035664.1"
                     /translation="SPHVASIFIAFCFSFPTGFFLNRHIAFPGSTLHGRIQLFRYFML
                     VIICIGLNIIFIKLFVEQFHIYPTIAKVLTTVIVVCFSYVTQKNFTFKIEESE"
BASE COUNT         2321 a         2976 c         2792 g         2674 t
ORIGIN      
        1 ggtatattat aataggtgat tgccggcgcc tcgccatatc cgaccaggcc gttgagttcc
       61 agcctgacga tgagggtagg ctgatgggtc ttggtggccc tggaaatggt aaacggatgt
      121 tggaacggga gactataaga ataatgtgta cagatcatgg ggggcaaaga taagggtaag
      181 cgccgttcgg accccttcgg aaattaccat ccgggctggg gtttccacga ccttttttgc
      241 agaatcgaga aaaattttcc cactccggga agagattcgg caaagatgcg cttccaaaat
      301 attgaaaatg aatagattgt gttttggcac tcttttgctt acttattcag cattcacgta
      361 atccttacca gtatgaaatc gatcgttacc ttttctcaga cccttcgcat cacgttcttc
      421 gctatcattc ttttcgctgt tgccggtcag caggccaacg cacagcaaac gcaatccagc
      481 aatgccgttg ttactcccgc tctgttgctg agctttgacg gtcaggtaga gaacagctgt
      541 gcaaatctca cctgggtcat ggaaaatgaa acgaattgta aaatgttcgt gatcgaaagg
      601 gccggcgaaa agggcggtta tgactcgatc aatgttgttg aaggtatgaa caacagcaat
      661 cagacgactt atactttcga agatgagaat atggtcatcg gtaacaacta ctatcgtctt
      721 cgtcaggtgg accgtgatgg cgtcgttcgc tactctaaga tcgtgacgct ttataagaat
      781 gctcccgctg ttgcagccaa tacgataggc atctatccca accccgctac ttcgactatc
      841 aattataccg ttgcttccac tacttcccag caggtgatcg tccaggtgtt cgatatctcc
      901 ggtgttgttc tgctgacgac ccagcaacaa ctgtatgccg gtaacaacca gcagactgtt
      961 ggtctctccg gtctgaagaa cggtaactat ttcctgcgtg taaccaaccg tgaaggttct
     1021 acccaatatg tacagccctt cgtcaagata atgtagaagc ctgaaaaatt taaccccttc
     1081 tcccgcagcc ggttgccgct ttcagcgcgc cggctgtaac tattcaggga accagcgccc
     1141 gcagttctcc acctcccctc tgaattccgc attaaaggtc ttttctttca gcagctcttc
     1201 cagcgtcagg tgcatacggt accgccaata ataatgcggg ttggccggca cattgatccg
     1261 ctcgtcatgc gggttctcgc ggcggaattc gtcgcttata cccatgatat cctgcagttg
     1321 gaagatcgcc cactgggcgg gcgaatgcaa atgttgcaag acgatggctt tattgatcca
     1381 gggttcgcaa aagaccggcg cttctcccca ttggcccagc tcccggttaa agaaccgctg
     1441 cgtgatcgtt cggtcttctt cccaccagcc gcgaatcgtg ctcatgtcat gggtggaagg
     1501 cgtcaccacc gacagatacg gggcgtgcgc cggatgcgag aattccttct gcgggttttt
     1561 cggcatgcgc tggacttcca ggctcaggat ccccagctgc cgcatcacat ccggtacgct
     1621 ccggggcacc atgccaagat cttctccgaa aaccagcata ttggtcgcgc gtttcagcgc
     1681 cggcagcttg cgcattgcct gacgctgcca gaaatcgtcc tgcctgctat agaaatagtt
     1741 gacataaagg tctttcagct gttgttgggt gtgccattcc aggtaacgga aagaggaggt
     1801 gtgttccatc gagatccgga aatggtattc ctggccttcc gaaccctcta tcccgagtaa
     1861 aatgacgttc gatacgaggt cgtaaagccc ctgcctgatc ttctcgttgc agcttcccgg
     1921 cggctgatgg ccgaaccact gctctatctt ccgctgtgta tcgaagggct cccgaaggac
     1981 ataaaagccg ttcccggtag gatgcagaaa gttgtctttg atgtgatccg attcgttccc
     2041 gaacagctcc cacagaacag cttcattgat aaagggccgg gtataacggt catggtcgaa
     2101 ccagatgttc cgctggtcga attccacgcg gtacacgggg atagccggaa cgaaacgacc
     2161 caggatgcct tccacgacgt ccatggggat gctccagatg cggaaaaagc cgaggatatg
     2221 atcgatacgg aacccgtcga aataattgtt catatgcgca aaccgtcgtt tccaccagga
     2281 atagccgtcc tggcgcatct cctcccagtt gtaggtcggg aagccccaat tttgtccttt
     2341 tacggcaaaa ttgtccggtg gtgcgcccgc ctgcatttcc atatgataaa gccggggctc
     2401 tacccaggca tcgcagctgt atcggaagat accgatcggg atatcgcctt tgaggatgac
     2461 cccgttctca tgcgcatagt cgacggcctc cctcagctga agatgcagat gatactgcgt
     2521 gaaatagtgc agagcgatgg aatcgtagtg ccgcgtaccc ggagccgtat accggagaat
     2581 ggcgtcctca tcatagttgt tgtgcatctt ccaacgggtg aaatccgggg taccgtgttt
     2641 gtcccggaga tagcagaagg cggcataggg caccagccag ttacggtgtt gccggaaaaa
     2701 tgtctggaaa tccggaacgt ccttgaattc ttctttctgt aattcgtata attctttaat
     2761 agccagcaat ttgatcttca tcacctgctc gtaatccact tccggaaggg catttagctc
     2821 cttttgtttt ttatgcaggg gtttgatcag ctgggcatat ttcttacctg cgcattgttc
     2881 caggttgagg tacagcgggt gtagggcaaa cgccgatatg gccgcataag gatatgagtc
     2941 ctgccaggta tggctggcgg tggtatcctg cacgggaagg atctgaacga gacgcagacc
     3001 gcatttgacg gcccagtcga tgagagcctt cagatcggtg aactccccta ccccgaacga
     3061 tcgtttgcta cgcaggctga agacgggtac cgaaacgccc gcacccttcc agccccggtt
     3121 gggcatatgg acgaatccgt catgcaggat gctgatctta ttcggtctgg cgtcaccggg
     3181 aagacggcgg ttcggcccat cttcaaaccc gatcagccgg cgatcctttt tatgataaac
     3241 agcatacttg tattcgatgg gaaaggcctc gggagggatg gaaagcgcca gcgtccacca
     3301 gtcgccatcc ggagagagca gcagagggtc ggcctcatcg gcgccccagt catgcagcgc
     3361 gccggcgtta ccaaggagtc cgacgacttc gtccgccgcc agaagcgggg cctttacccg
     3421 gaagacatgg gttgtaccgg tcttcttttt ctgttttttc cctttatgcc ggggcagcag
     3481 cacctgtctg aagggagaag tataaaagac gttctcaaac tccccggcat agttccagga
     3541 gtcgatcacc tgcagctctt cgacggtggc ggccggcgtt tcgatgaact tgtcatcgcc
     3601 ccattcctcg gtcagcgttc catcgatgtt cttcaggata tagtgatatt gcaccggcga
     3661 cgatgggata tcccccaaaa ctacgctccg atgccagaat tcgccgttga catattcaag
     3721 tgggatggcc gcagcgatct ccccgttgcc caattccggg agattccccg ataaaaggat
     3781 tgactggccg ggatgtgtat aaaaacgcag atagaaatcg agtttgagcg ttgtcatctt
     3841 ggtgtgatct tttgcaattt gctggccata tgccgaaaac ccgggagcca ttttaccccc
     3901 gacgccatcg ttaatgtgta tttattacat tatatcgcca atttatggat aaattccttt
     3961 ccttgtcgct ttaaaataat ttttgcccta aattcgcaca aatttctctc atggaacagc
     4021 aaaagaagaa taccggcgcc tattggatct tgttttttgt ctccgcggct gcgttgatct
     4081 gggccgttgc cacgcatttt tcctatctca ccctgattct tcctttcgta tgcacatcct
     4141 tcgttaaggc gatggatatc atgtaatgtc ccttcactcc tactaccagg gtcaggtcga
     4201 cgcctgtcgg atggagattc gtcgactgca aaataagttg aatagcctgg ctattctgcg
     4261 ggtactcgtt tttgcctggt tcgccgggtc cgtctatacc ctgatacagg ggctttcggc
     4321 cggctggctg gtttcggcga tcctggcagc cgcaggtttc atcggttgcg tcaacatcta
     4381 tctccgttgg aaagacaagc gccggttgca ggagaagctc cggtatgtct gtgaaaacga
     4441 gctggctcag ctgggagggg ccgaaaaccg gtttccggat ggcgggtcct ggctggatag
     4501 cgccaattat ctcgatgacc tcgatatctt cggtccacga tcgctttttc acgtgctgaa
     4561 ccggaccacc acggcgccag gcgcctccga tctggcggca cggctgcgtc agccttttct
     4621 catgtccgga accattcaac agcagcagga agcgatccgt gtcctcacgc cccaggcggt
     4681 aatccggcaa ctccttacgg cgactggaat ggtcagcggg gaagacgcgc aacgggcggg
     4741 gcagtctggc ggtcataacg cttcggaaaa caaaaacctc gatgattggt tggatacgcc
     4801 gccacgtctg gtcaaccgtc cctggctttg gctagtcatc gccgcactct ccctgttcaa
     4861 cctctatggg ctgtatctct tcttcgacaa tggcaattac accctgatca ttgccggcat
     4921 catcatttcc cgcgcgataa cgggcgcctg gtcgcgctat atcaacaccc agcaccgtct
     4981 aatcggtcac cagcatgcgc tgctcgacca gtatgccgga attctttccg tgttcaacca
     5041 gaccgcaacc acgggctctc atctgttgca gcaggtacag gagagtacgg aaaaggcttc
     5101 ttcggctatg cgccggcttt cccggttgtc ctcctatttc gatcagcggc tcaacctcct
     5161 ggtcaacctg atcctgaaca ccttcttttt ttatgatctg ctttgcatga tcgcgctgga
     5221 gcgctggaag actgccaacc gggcggggtt cccggcgtgg atcgatgccg tcggtacggt
     5281 cgaggcgctc aattcgctgg ccggtttcgc gtataatcac cccgaataca tctatccttc
     5341 accggtagcc ggccctcttc tcatcgaagc taccggtctg gcacatccga tgatccccgc
     5401 cacgcgccgt atagccaacg acatcgccat tggccgcgac cagcggctca tcctggtcac
     5461 ggggtcgaat atgtcgggca agacaacttt tttacgaacc gtgggcgtca acctgctgat
     5521 ggcgcagtgc ggtctgccgg tatgcgccac gacattcacg ttcaccccga tgcagctgtt
     5581 gacctcgctc aggatcaccg actcgctgca ggaacagacc tcttatttta tggcggagct
     5641 gaataagctg cagatgatca tccaccggct tcgcgcgggc gaaccggcgc ttgtcctcat
     5701 cgacgagata ctccgcggta ccaattccga ggacaagacc tatggttccg aacaattcat
     5761 ccggcgattg ctgcaatatc cctgtctctc ctttttcgcc actcacgatc tgacgctggg
     5821 acagctggaa ggcgaaatgc ccggcgcaat cagcaactat tgtttcgaga gcgtcatcga
     5881 aggcgaggag ctgcacttcg attaccggct gcgacgcggt gtcgcgcgca accgcaatgc
     5941 gtcattcctc atgcgaagaa tggatatcat ataaatgatg agccccgctg gtgcggggct
     6001 cgtatcgatt acttcacttc ttcgaactcg gcatctgtta cattctcgct gccggtattg
     6061 gcctggccgc cgccatcggg cccattgggc ccgcctccgg gctgtccgcc ctgctgttgc
     6121 gacgccttgt acatttcttc ggaagcgacc gtccatgcat tgtcgagcgc ggtgatcgcg
     6181 gcatcgatag ctgcgacgtc ttccgattta tgggcctcct tcagtttgga aagggcggtt
     6241 tcgatcggcg ccttcttatc ggcaggcagc ttgtccccga attccttcag ctgcttttcc
     6301 gtttggaaga tgcggctgtc ggcctggttg agcttgtcga tcttttcgcg cgtcgccttg
     6361 tcgctgcttt cgttggccct ggcttcggct ttcatctttt cgacttcttc cttgctcaga
     6421 ccgctgccgg cttcgatacg gatcttctgt tccttaccgg tgcctttatc cttggccgtg
     6481 acatgcagga taccgttggc atcgatgtcg aagatgactt cgatctgcgg tacaccgcgc
     6541 ggggcaggcg gaatatcgtt gagattgaac atgcccaggc tcttgttctg cgaagccatg
     6601 ggacgctcgc cctgcagcac atggatctga acgccgggct ggttatcgct cgcggtggag
     6661 aaggtctccg atttcttggt cgggatggtc gtattcgaat cgatcagtct cgtcatgact
     6721 ccgcccatgg tctcgatacc cagcgacagc ggggttacgt ccagcaggag tacgtccttt
     6781 acctcacccg tgagaacggc gccctggatg gccgccccga cggctaccac ttcatccgga
     6841 ttcacaccct tgttgggctt tttgccgaag aatttctcga cgagatcctg cactttcggg
     6901 atacgcgtac tgccgccgac gaggatgacc tcgtcgatct gcgaagccga tattccggca
     6961 tctttcaacg cctgctcgca gggcttgaga catctttcga aaagggaatc ggccaactgt
     7021 tcgaatttgc tccgggtgag tttttttacc aggtgtttgg gcactccgtc cacggcggta
     7081 atatagggaa gattgatctc cgtttcgctc gatgaggaca gctcgatctt ggctttctcg
     7141 gcggcttctt tgagacgctg taaagccatc gggtccttcc tcagatcgac ggcttcgtct
     7201 ttcctgaact cgtcggccag ccaatccatg accaccttgt cgaaatcgtc accacccaga
     7261 tgggtatccc cgttggtgga tttaacttcg aaaacgccat cgcccagctc caatatcgaa
     7321 atatcgaacg taccgcctcc aaggtcgaat acggcgatct tctcgtcttt cccctgctta
     7381 tccagtccat aggccagcgc agcggcagtg ggctcgttga cgatacggcg aacggtgaga
     7441 cctgcgatct cgcctgcttc cttggtagcc tggcgctgtg cgtcgttgaa ataggcgggg
     7501 acggtgatca ccgcttccgt cacttcctga cccaaataat cttcggccgt cttcttcatc
     7561 ttctgaagga tcatggccga tatttcctgc ggcgtataca gacgaccgtc aatgtcaatg
     7621 cggcatgtat tgttgtcgcc tttcactact ttatagctcc agtgtgtaat ttcgttggtt
     7681 acttcgtcgt atcggcgtcc catgaacctt ttaaccgaca tgatggtgtt aacaggattg
     7741 gtaatggcct ggcgcttggc aggatctccc acctttcttt ccccgttttt gaggaaaccc
     7801 acgatggaag gtgtggtgcg atgcccttcg tcattggcga tcaccacggg ctcattgcct
     7861 tccatgacag ctacacaact gttcgtcgtg ccgaggtcaa ttcctataat ttttcccata
     7921 taatttatct gattttaaag gttttgtaag taattccggg gtaccatgac aactatgatg
     7981 ccacgcccga aaaagtgaaa aaatgtcaga ccttttgtca ggccgggtcg cacgctctaa
     8041 cggcgcttta gcggaccact tccaatacgg gtttcagcga attaccccgg aatacgtagt
     8101 cggccacctc atcgaggatc ggagtgatgg agttgaagga gatgcctgta cccgccattt
     8161 tgaccatgca aatatcattt tcaccgtccc cgactgccac ggaatcggcc aggctgatcc
     8221 caaaacggtg gaggatatac tgaaacatat ttgatttaca atgatcatga tcgcactggc
     8281 tgaagcggtc gcgtaacagc tgggacggta ttttgacctc accggtagca acgcttttcc
     8341 ggaattccag ctcatttccc agcgtgaaat caagtccgag caaatttttg atatgattgg
     8401 ctatcgcatg atagctgtca ctgatgatcc cgcagacata gccccggacc tgtaactccc
     8461 tgatcacctg gaccgcgtcc ccgatcaggg gaatggattc cacaacgtcg atcagctcgg
     8521 caaaggtcct tccctccagc agccgtgcga tcttttgggt ctttatatag ttgcttttct
     8581 gctggcccag gatatcggcc agctcgcggg taaagccgaa ctgtttggcg gctgcctgga
     8641 taaagctgcc ctgcagaagc gtatggtcca tatcgaagac gatcattttt ttcggcgccc
     8701 ggaccagttc cttgagtgcg aattccacct gctcgcgcat ggcaggcggg gcgtcctcct
     8761 gctggcggcg gctgtcgaag atattcaccc gtttgaatat ggcattggcg acctgtttgg
     8821 ccatacggct cagcacatga agcggctgcc ggtcgttgcc gacctcgccg atacagactt
     8881 ccgcgatacg cgcctgcacc tggtgcatat cgatcagcaa cccgatatca accccgtagt
     8941 cgttgtcaaa ctccaccttt tgcaggatcg attttttgcc cgcgatcatc ccgctcagcg
     9001 gttgtttgaa tttcagcagg tgcgggaaaa agaattccag caacggcttg gcgaggatct
     9061 ccgccatccg gcctccggat cgctcgaaat aggatttcac gaaatcggcc tcatcgcgga
     9121 tcagcggggc cgtgagcatt cctacgatgt ctacttcata atcggggata tcggcctcca
     9181 tgaaaacgac gatatcgttc ttcgccatca gtaatccttc cctcatcgaa tctcccttac
     9241 cgacgtgtgt gcttgttatg accttcactt cttcgttcct tgcctccgct atcgtgtcat
     9301 cggtagacat atcgtcgacc acgatgatct cgtccaccaa ctggtttcgc ttggcctgac
     9361 ggatcgcttg gcggatcgtc ttttcttcat tgagggcggg tatcacgact gtcaccatag
     9421 gcgtcttttt gttcaaaagc aggtaaagtt acgaattgtt gagggctata acttcccaaa
     9481 tatcgtaccg gcaatttcat gtaaagattt gaaaaacaag aattaaattt agggctgtca
     9541 attttaatcc aggtatgcca attgctcccg acatctacag gaggatcgtt tcggccaagc
     9601 tgtacatcga cggaaatttc cgcgagcgtc tcgatctgga ccggctggcg cgtgaggcct
     9661 gtctctcccg gtatcatttc caccggctgt tcacccgcat ctaccgggtg acccctcacc
     9721 aatatctcac ccgcaaaagg atcgaggctg cgcagctggg tctggccggg caggcgcgga
     9781 cggtgaccga ggtctgtaac gatgtgggtt tcgaaagcat cggctccttc agcagcctgt
     9841 tcaaaaaaga gatcggtcat gcccccgcca gttatcgttc gctcacacac ggccgcaaca
     9901 gacaggtgaa ggactcgccc cgttcgttca tcccgcattg tttcatagaa accctgacga
     9961 caaaaagcaa ttctcaagaa gccgtttccc ccgcgaaatc ctagctttac cggcaaatac
    10021 tacaaccatg atcacacgtg tctcgcacgt cacctttttc gtgaccgacc agcaaaaagc
    10081 ccatgaattc tatgtgggaa tattagggtt cagggtcaat accgacgcca ctatggagaa
    10141 tggattccgg tggctgacgg tcagtcctcc cgatcagccc gacctggaaa tagcgctgat
    10201 cgctccgctc aacggcggta tgggttacga tgaggaaacg acacaggctt tcagggtcct
    10261 gctggagaaa ggcgccctgg gcgcgggcgt catggatacc cccgattgcc gcgccaccta
    10321 tgaagagctg agatccaaag gagtcggttt caaaggcgag ccgaaagacc aattctacgg
    10381 gacggaagcg atcctgttcg atggtatcgg caactggttc tccatgaccc agcgaaaata
    10441 aaccagcatt acccgatcgc acggaaatta ttcggattcc tctattttaa aagtgaagtt
    10501 cttctgcgtg acatagctga agcagacaac gatcacggtc gtcaggacct ttgcgatcgt
    10561 cgggtagata tgaaactgtt ctacgaacag cttgatgaag atgatgttga gcccgataca
    10621 gatgatgacc agcatgaaat aacgaaacag ctggatacgg ccgtgcaagg tgctgcccgg
    10681 gaacgcgata tgccggttca ggaaaaaacc agtggggaag ctgaagcaaa atgcgatgaa
    10741 gatagaggcc acgtgaggag aca
//
LOCUS       DASYSV010000110         3620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_792448, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000110 DASYSV010000000
VERSION     DASYSV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792448"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1420
                     /locus_tag="VGQ51_03555"
     CDS             257..1420
                     /locus_tag="VGQ51_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV9035665.1"
                     /translation="MSNILIIDDEKAIRKTLSEILSYEGYKIDEAGDGEEGLKKVKEK
                     EYDVILCDIKMPKLDGIEFLEKTRETHPDIPVIMISGHGTIETAVEAVKKGAYDYISK
                     PPDLNRLLITIRNAMDKTTLVAETKVLKRKVSKVQEMVGSSTPIHKIKETIEKVAPTE
                     ARILVTGENGVGKELVARWVHEKSNRAGGALVEVNCAAIPSELIESELFGHEKGSFTS
                     AVKQRIGKFEQANGGTLFLDEIGDMSLSAQAKVLRALQEGKITRVGGDKDLNVDVRVI
                     AATNKDLLKEVDEKNFRLDLYHRLSVIIIHVPSLNERKDDIPILVEKFLVDICGDYGI
                     AKKSIDEDALKALQQHNWTGNIRELRNVVERLIILSGKTITQSDVENYVLPKK"
     gene            1426..2097
                     /locus_tag="VGQ51_03560"
     CDS             1426..2097
                     /locus_tag="VGQ51_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV9035666.1"
                     /translation="MRTVYCISGLGADHRIFAKLNVPGIDFSPLSWMAPSRHEDIGTY
                     ANRMREGVPEDHPVLLGVSFGGMMAIEMAKKIPGATVIIVSSVSTRSELPLWMRLGGY
                     LHIDRLSPAAVIKRGAILTRLENHKLGVESEEEALLCQEFRDHIDGRYLNWAIGVILR
                     WRNEWKPASFYHFHGGKDHIFPIRTVTPTHIIGDGGHFMIYNRAEEIGRLLGGILAKL
                     SWSML"
     gene            2147..>3620
                     /locus_tag="VGQ51_03565"
     CDS             2147..>3620
                     /locus_tag="VGQ51_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEV9035667.1"
                     /translation="MSSHQLLVLVLIQVVILLLPSIGASKLFEKAGVPGWKAFVPFYN
                     TWIMLQLAERPKHWVFWQIIPVVGWFISLGIYVEFVKTYGKFKLWEHAAAALLPLIYF
                     PIIGFDPKVKYKGAEPVRKHKKGTAREWIDAGVFAIVAATLIRTFVFEAYTIPTGSME
                     KTLLINDFLFVSKFSYGPRIPNTPLSIPFVHNTLPVTNGNSYVEWIKLPYTRWFPSPV
                     KRRDVVVFNFPAGDTVINLPEYQSLRPYYDVCRELGRGNIDSGRAIVLGNPDQYPLVI
                     RPVDKQENYIKRCVAIAGDTLQIRNQEVYINGEKQDLPPESETYYTVETKGQPLDEDA
                     MKSAYDVDINNSDEVRATGTPNQYDMLLTWDAHNKMVASGLAKSIVPDIDSQQSVYPY
                     SPSSPWSRDNYGPIWIPAKGATLTLTAQNYPIYERAIRVYERNEFELRDGKFWLNGQP
                     VTQYTFKMNYYWMMGDNRHGSQDSRYWGFVPEDHVVGEAAL"
BASE COUNT          924 a          903 c          984 g          809 t
ORIGIN      
        1 ataggggaat attttttgga tgttaaattt ttcggttttc agtagtcatt ggatagcgct
       61 aaaatacaaa ccggggggtt ttcaaacaag gggtttttac gatcgccggc ttggtttttt
      121 tacggcccgg ttcgtaactg attggttgtg aatgcccatt ttttgcgcct gcaaaaggga
      181 aacaccgtcc aattccacaa atccgtaaat ttgaaactcc taagattatt attaaaacaa
      241 aaagtcaggc caatccatgt cgaacattct aatcattgac gacgaaaagg cgatacgtaa
      301 aacattgagc gagatcctat cttatgaagg ctataagatc gatgaggccg gcgatggcga
      361 ggaggggctc aaaaaggtga aggaaaagga atacgatgtc atcctttgtg atatcaagat
      421 gccgaaactc gatggtatcg aattcctgga aaagacaagg gagacccatc ccgatatccc
      481 cgtcattatg atatcgggtc atggaaccat cgagacggcg gtagaggcgg taaaaaaggg
      541 cgcctatgac tatatctcca aaccacctga tctgaaccgt ctgctcatca cgatccgcaa
      601 tgcgatggac aagaccactc tcgtggccga gaccaaggtg ctgaaacgga aggtcagcaa
      661 agtccaggaa atggtaggct cttccactcc tatacacaag atcaaggaga ccatcgaaaa
      721 ggtggcgcct acggaagcgc gcatcctcgt cacgggagag aacggggtag ggaaggagct
      781 cgtcgcgcgt tgggtgcatg agaaaagcaa ccgggccggc ggagcgctgg tcgaggtcaa
      841 ttgcgccgcg atccccagtg agctgatcga gagcgagctg ttcggtcatg aaaagggttc
      901 ctttacttcc gccgtcaaac aacggatcgg gaagttcgaa caggccaatg gcggaacgct
      961 tttcctcgat gagatcggag acatgagtct gtcggcccag gccaaggttt tgcgggctct
     1021 tcaggaaggt aagatcacac gcgtaggcgg cgacaaagac ctcaatgtgg acgtgcgggt
     1081 catcgcggcg acgaataagg acctcctcaa agaagtggat gaaaagaatt tccggctcga
     1141 tctttaccac cggttgagcg tgatcatcat ccacgtccct tcgctcaacg aaagaaaaga
     1201 cgatatcccc attttagtcg agaaattcct cgtagacatc tgcggcgact atggcatcgc
     1261 gaaaaaatcc atcgacgaag atgcgctcaa ggctttgcag caacacaact ggaccggtaa
     1321 tattcgcgaa ttgcgcaacg tggtcgaacg cctgatcatc ctgtcgggca agacgatcac
     1381 acaaagcgat gtcgagaatt acgttctgcc gaaaaaataa gggtgatgcg aacggtgtat
     1441 tgcatcagcg gtctgggcgc ggatcatcgg atctttgcca aattgaatgt tcccgggata
     1501 gatttttcgc cgttgtcctg gatggcgcct tcgcggcatg aagacatcgg gacctatgcg
     1561 aaccgcatgc gcgaaggtgt cccggaggac cacccggtgc tgctgggcgt ttcatttgga
     1621 ggcatgatgg cgatcgagat ggcgaaaaag atccccggcg cgaccgttat catcgtctcg
     1681 agtgtcagca cccgaagcga gcttcccctg tggatgagac ttggaggcta tctgcacatc
     1741 gatcggttgt cacccgcggc cgtgatcaaa agaggggcta tcctgacccg gctggaaaat
     1801 cataagctgg gggtcgaaag tgaagaagag gcgctattgt gccaggagtt cagagaccat
     1861 atcgacggga gatacctgaa ctgggcgatc ggggtgatcc tgcgttggcg caacgaatgg
     1921 aaacccgctt ctttttatca ttttcacggc ggtaaggacc atatctttcc catacgcacg
     1981 gtgactccca cacatatcat cggcgacggc gggcatttca tgatctataa ccgcgctgag
     2041 gagatcggtc ggctgctggg gggtatttta gcaaaacttt cgtggtcaat gctttgagag
     2101 gccaaatatt agattaaatt tgccgcaatt caaaaatatt ccaggaatgt catctcatca
     2161 gctgctggtt cttgtgctta tccaggtcgt catcctccta cttccctcta tcggtgcttc
     2221 caaactcttt gaaaaagccg gtgttccggg ttggaaggcg ttcgtcccct tttacaatac
     2281 ctggatcatg ctgcagctgg cagagagacc caaacactgg gtattctggc agatcattcc
     2341 cgtagtgggc tggttcatct cgctgggtat ctatgtggaa ttcgtcaaga cctacggaaa
     2401 gttcaagctc tgggaacatg cggcagccgc gctgctcccg ctgatctatt tcccgatcat
     2461 cggattcgac cccaaggtaa agtataaagg agccgagccg gtgcgtaaac ataaaaaagg
     2521 tacggcgcgg gaatggatcg atgccggcgt tttcgccatc gtcgcggcga cacttattcg
     2581 caccttcgtt ttcgaggcct ataccattcc tacaggctcg atggaaaaga ccttgctgat
     2641 caatgacttc ctttttgtca gcaaattcag ctacggcccc cgcatcccca acacgccatt
     2701 gtctattccc ttcgtccata acacgctgcc cgttaccaat ggcaactcgt atgtggaatg
     2761 gatcaagttg ccctataccc ggtggttccc cagcccggtg aaacggcgcg atgtggttgt
     2821 cttcaatttt ccggcaggcg ataccgtcat caatcttccc gaataccagt cgctgagacc
     2881 ctattacgat gtatgccgcg aactgggcag gggcaacatc gattccggca gggcgatcgt
     2941 gctgggcaat cccgatcaat atccgctggt gatccgtccc gtggataagc aggagaatta
     3001 tatcaaaaga tgcgtggcga tcgccggcga tacgctccag atcaggaacc aggaggtcta
     3061 catcaatggt gaaaaacagg atctccctcc cgaatccgag acctattaca ccgtagagac
     3121 aaagggacag ccgctggacg aggacgccat gaaaagtgcc tatgatgtcg atatcaataa
     3181 tagtgatgaa gtgcgcgcga cggggacacc gaaccagtac gacatgctcc tgacctggga
     3241 cgcgcataat aagatggtgg cgagcgggct tgccaaaagc attgttccgg atatcgacag
     3301 tcagcagtcg gtgtaccctt attcgccgtc ctcaccctgg tcgcgggata attacgggcc
     3361 gatctggatc cccgcaaaag gtgcgacgct gacgctgacc gcgcagaact atcccatcta
     3421 tgaacgcgcg atccgggtat atgagcgcaa cgaattcgag ctgcgcgacg gtaaattctg
     3481 gctcaacgga caaccggtga cccaatatac tttcaaaatg aattattact ggatgatggg
     3541 cgataaccgt catggatcgc aggattcgcg gtattgggga ttcgtaccgg aagaccatgt
     3601 agtgggcgag gccgcgctca
//
LOCUS       DASYSV010000111         6106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2328715, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000111 DASYSV010000000
VERSION     DASYSV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328715"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VGQ51_03570"
     CDS             complement(<1..956)
                     /locus_tag="VGQ51_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEV9035668.1"
                     /translation="MRRKTIPRDISWLSFNGRVLQEAADPTVPLRERIRFLGIFSNNM
                     DEFYRVRVATLRRMSEYSGAKSKLNMHLEVSPERILQDIQSIVLEQGDEFDAIWSGIR
                     KEMENEKIFLVTETELDPRQQQFIQNYFEEEVRSNTIPLMIESIPTFPYLRDKSLYLG
                     VVLSRRDNSMKRKYALVEIPSRVLGRFVILPSPAPEEHHIILLEDIIRFNLRNIFAYF
                     GYDSYESWVFKVTRDAEIDLDNDVSTTLIQKIEKGLKNRRKGKPVRFVYDEQLDKGLL
                     EYLLKRLNLSHKSGNLIPGGRIHNFRHFMDFPDVFHRQGHRR"
     gene            1135..3063
                     /locus_tag="VGQ51_03575"
     CDS             1135..3063
                     /locus_tag="VGQ51_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEV9035669.1"
                     /translation="MLYKYAGWLIGLLSITCAATAQTPEGSWSVYGQDAGGMRYTSLT
                     QINDRNVTQLKPAWTFRTGELKKYEGNYALQKAAFETTPILIGRTLYLSTPSDRVIAV
                     DAATGQQRWVYDPGVDLNADHSEISNRGVAAWPAGASTADRIFIGTIDGRLIALNAAT
                     GRPSASFGKDGIVDLRSGLGEDIAETSPPVVAGNLVIVGSSLGDNQRFDYPPGTVRAY
                     DVKTGAQVWSWDPIPQDAGDTAYASWQGPKAHRTGGANAWSILSYDAARDLLFVPTTS
                     PSPDYYGGERKGSNHYANSIVCLRASTGKLRWSFQVVHHDLWDDDIAAQPVLAELDRG
                     NKKVPVVIIGTKMGHIFVFNRETGVSLFPIEERPVPASTVAGEQAWPTQTFPVLPAPV
                     GIQHLSLDDAWGPTEADRDEARRRIAGYRNQGPFTPPSFEGSIMVPGNVGGINWSGMC
                     YDPIHECLITNIERVPAVIRMIPRDSLDLVERAEPSVLRAETGRQQGTPYVMKRDYLL
                     KTDERGYVMQVKPPWGTLLAIDLHTGLKKWEVPLGYMMDPAKYPEARQWGSINFGGAI
                     VTAGNLIFIAASMDGHLRAFDRRTGKVLWEYALPASAQATPMTYSLDGKQYVVIAAGG
                     HGKLHTKQGDYVMAFALP"
     gene            3140..3376
                     /gene="rpmB"
                     /locus_tag="VGQ51_03580"
     CDS             3140..3376
                     /gene="rpmB"
                     /locus_tag="VGQ51_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV9035670.1"
                     /translation="MARVCQITGKVPVTGHKVSHSNIKTKRRFLPNLQTKRFFLAEED
                     KWITLKLSSEGLRTINKNGLYNVVKKLRANGEKI"
     gene            3426..3608
                     /gene="rpmG"
                     /locus_tag="VGQ51_03585"
     CDS             3426..3608
                     /gene="rpmG"
                     /locus_tag="VGQ51_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV9035671.1"
                     /translation="MAKKGSRVQVILECTEHKHSGQPGTSRYITTKNKKNTPERMELK
                     KFNPILKKVTVHKEIK"
     gene            3611..3784
                     /locus_tag="VGQ51_03590"
     CDS             3611..3784
                     /locus_tag="VGQ51_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HEV9035672.1"
                     /translation="MAKAAKTAIKGKDAKAAAEAKNWTKVIKAVRSPKTGAYTFKEAI
                     VHKDRIKAYLAEK"
     gene            3860..4828
                     /gene="ftsY"
                     /locus_tag="VGQ51_03595"
     CDS             3860..4828
                     /gene="ftsY"
                     /locus_tag="VGQ51_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV9035673.1"
                     /translation="MGLFDKLFGKKEKQSLDEGLQKTKEGFLSKITKAIAGKNTVDEE
                     VLDQLEEALVSADVGIDTTIQIIQRIEKRVAKDKYINTSELNSLLQQEIEAILADAGD
                     QGYRDFTIPSGQKPYVILVVGVNGVGKTTTIGKLAYNFKRAGRSVILGAADTFRAAAV
                     DQLTIWSERAGVGIVKQGMGSDPGAVAFDTVQSGVARGTDVVIIDTAGRLHNKAYLME
                     ELSKINRVIKKVIPDAPHEVLLVLDGSTGQNAIEQARQFTAATDVTALAITKLDGTAK
                     GGVVLAIANQFKIPVKFIGVGEKMEDLLVFDKHEFVDSLFNLEGQR"
     gene            complement(4846..5322)
                     /locus_tag="VGQ51_03600"
     CDS             complement(4846..5322)
                     /locus_tag="VGQ51_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035674.1"
                     /translation="MRKTLLLASIFAFIFSTRGFAQAEGNSYSTAIGIKFWPGAISIK
                     HFVSDDNALEGLLNFWDGGFRLTGLYEIHGDISGADGLKWYVGPGAHFGVFNGYGYND
                     YYYNSGTVSIGVDGVLGLEYKFSGAPIAASLDINPYLELNHVYLGVWGGLGLKYTW"
     gene            5457..>6106
                     /locus_tag="VGQ51_03605"
     CDS             5457..>6106
                     /locus_tag="VGQ51_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV9035675.1"
                     /translation="MKTRSLQKDKVNIITLGCSKNMVDSEVLSGQLRANAIDVVHENS
                     KKDHNIVVVNTCGFIDKAKEESINTILRQVDLKKKGRLDKVYVTGCLSQRYREDLEKE
                     IPEVDAWFGTMELPLLLRRMDADYKSELLGERMLATPQHYAYLKISEGCNRTCAFCAI
                     PLMRGGHVSRPMDDLVREAEGLVRRGVKEILLIAQELTYYGLDLYKQRMLPQLLHRL"
BASE COUNT         1526 a         1577 c         1698 g         1305 t
ORIGIN      
        1 ttcctccggt ggccctgccg gtggaaaacg tcgggaaaat ccatgaagtg acggaaatta
       61 tggatacgcc cgccggggat gaggttgccg cttttgtgcg agaggttcag acgtttgagc
      121 aggtattcca gcagaccctt atccaactgt tcgtcataca cgaagcggac gggtttgcct
      181 ttgcgtctgt tcttcaggcc tttttcgatc ttctggatca gcgtggtcga gacgtcgttg
      241 tccagatcga tctccgcgtc gcgggtcacc ttgaagaccc agctttcata gctgtcatag
      301 ccgaaatacg cgaagatatt ccggaggttg aaccggatga tgtcttcgag aagtatgata
      361 tggtgctctt cgggcgccgg agagggaagg atgacgaacc ggcccagtac gcgggagggt
      421 atctccacga gggcatactt tcgcttcata ctgttgtcgc gccgcgacag gaccacgccc
      481 agatacagcg atttgtcccg cagatagggg aaggtcggga tgctctcgat catcagcggg
      541 atggtgttgc tgcgaacttc ctcctcgaaa tagttttgta tgaattgctg ctgccggggg
      601 tccagctccg tttcggtgac gagaaagatc ttctcgttct ccatttcctt gcggatgccg
      661 ctccagatgg catcgaattc atcgccctgc tcgagcacga tggactggat atcctgcagg
      721 atgcgttctg gagacacctc gagatgcatg ttgagcttgg acttggcgcc gctgtattcg
      781 ctcattctac gtaatgtcgc cacacgcacc cggtagaatt cgtccatatt gttcgaaaat
      841 atgcccagaa aacggatcct ttcgcgaagc ggcaccgtgg ggtcggccgc ctcctgtaaa
      901 acccgtccgt tgaatgaaag ccagctgata tcgcggggta tggtttttct tctcattgat
      961 tccttgattt cgcccatttt gcgcaaagta cccaatgcaa aagggataag taaattaagt
     1021 aattattatt aattactatg ccacgccatc ttttggtcac cggtggcggg gcattcgtca
     1081 aagaaattcg cggtgcgcgc agattctcat taggtttgat aaaaatcatt aaccatgtta
     1141 tataaatatg ccggatggct catcggatta ctgtcgatca cctgcgccgc aaccgcccag
     1201 acgccggaag gatcatggtc cgtatatggt caggacgccg gaggtatgcg gtatacctcg
     1261 ctgacgcaga tcaacgaccg gaatgtcacg cagctgaaac ccgcatggac cttccggacc
     1321 ggggaattga aaaaatatga agggaactat gcactgcaga aggcggcatt cgagacgacg
     1381 cccatcctca tcgggcggac gctctacctg tccacgccga gcgaccgtgt catcgccgtg
     1441 gatgcggcta ccggacagca gcgatgggtc tatgatccgg gtgtcgatct gaacgcggat
     1501 cattcggaga tcagcaatcg cggcgtcgca gcctggcccg caggcgcatc gaccgccgac
     1561 cgcatcttca tcggcaccat cgacggcagg ctcattgcgc tgaatgccgc caccggacgg
     1621 ccatccgctt catttggaaa agacggcatc gtcgatcttc gctcaggcct cggcgaagac
     1681 attgccgaga catcgccacc tgtcgtcgcg ggtaacctgg tcattgtcgg aagctcgctg
     1741 ggcgacaacc agcggttcga ttatcctccc ggaacggtgc gcgcttatga tgtgaagacc
     1801 ggcgcgcagg tatggagctg ggatccgatc ccacaggatg ccggggacac tgcttatgcg
     1861 agctggcagg ggccgaaggc gcatcggacc ggcggggcca atgcctggtc catcctttct
     1921 tatgatgccg cgcgcgatct gttgttcgta ccgactactt ctccaagtcc tgattattat
     1981 ggcggcgaac gcaaggggtc aaaccactat gccaattcga tcgtttgtct ccgggcgtcg
     2041 acgggcaagc tccggtggag cttccaggtc gtacatcacg atctgtggga tgacgatatt
     2101 gccgcgcagc ctgtactggc cgagctggat cgcgggaata aaaaggtgcc ggtggtcatc
     2161 atagggacca agatgggaca tattttcgtt ttcaaccgcg agacgggcgt cagcctgttc
     2221 cccatcgagg agcgaccggt accggcatcg accgtagccg gtgagcaggc gtggccgacg
     2281 caaacatttc cagtgctgcc ggcgccggtg ggaatacaac atcttagcct ggatgacgca
     2341 tggggaccga ccgaagccga tcgggacgaa gcacggcggc ggatcgccgg atatcgcaat
     2401 cagggcccct tcaccccacc cagtttcgag ggatctatca tggttccggg aaacgtcggc
     2461 ggcatcaact ggagcggcat gtgctacgac cccatccatg aatgcctgat caccaatatc
     2521 gagcgggttc cggccgtgat ccggatgatc ccccgcgaca gcctcgacct ggtggaaagg
     2581 gccgaacctt cggttctgcg tgccgagacg ggcaggcaac agggcacccc ctatgtcatg
     2641 aaacgcgact atttgttgaa gaccgacgaa cggggatacg tcatgcaggt gaagccaccc
     2701 tgggggacat tgctggccat cgatcttcat accggactga aaaaatggga agtgccgctc
     2761 ggctatatga tggaccccgc aaaatacccc gaggccaggc aatggggctc gatcaatttc
     2821 ggcggagcga tcgtcaccgc gggaaacctc atctttatcg cggccagcat ggatgggcac
     2881 ttgcgggcct tcgaccggcg gacgggtaag gtgctctggg aatatgctct cccggccagt
     2941 gcgcaggcga cgcctatgac ctattcgctg gatgggaaac agtacgtggt gatcgccgcc
     3001 ggcggacatg ggaagctgca tacgaaacaa ggggattatg tgatggcgtt cgcgctgccg
     3061 taagctttat gaaaaaactg ccatcttttt tggctaattc ccaatatttt ccgacatttg
     3121 caatccaaaa tttcacgtca tggctagagt ttgtcagata acgggtaagg ttccggtcac
     3181 cgggcataag gtgtcgcact cgaatattaa gaccaaacgc aggttcttac ccaatttgca
     3241 gaccaagcgt tttttcctgg ccgaggaaga caaatggatc accctgaagc tgtcttcgga
     3301 aggattgcgc acgatcaaca agaacggtct gtacaatgtc gttaaaaaac tgagagcaaa
     3361 cggagaaaag atctaaaacc atttcacccc gagtctaatt tatacctcgg tctaatttat
     3421 tcaacatggc aaaaaaaggc agcagggttc aggtgatact ggagtgcacc gagcacaaac
     3481 acagcggaca gccgggtacc agccgttata tcacgaccaa gaacaaaaag aatactccgg
     3541 aacgtatgga gctgaagaag ttcaatccca ttctgaaaaa agtaaccgtt cataaagaaa
     3601 ttaaataatc atggcaaagg ctgcaaaaac cgctattaag ggcaaggatg ccaaggctgc
     3661 cgcggaagcc aagaactgga cgaaggtgat caaggctgtt cgcagcccca agaccggcgc
     3721 ctatacgttc aaagaggcga tcgtccataa ggacaggatc aaggcatacc tggctgaaaa
     3781 ataatttcgg catagaataa acttaactga aaagcggacg gggcaaatag gctcgtccgc
     3841 tttttgcaat tggcaccata tgggattatt cgataagcta ttcggaaaaa aggagaagca
     3901 aagcctcgac gagggtctgc aaaagacgaa ggaaggattc ctgtcaaaga tcaccaaagc
     3961 catcgccggc aaaaacacgg tcgatgaaga ggtcctcgac caattggaag aagcgctcgt
     4021 cagcgccgat gtcggcatcg acaccaccat ccagatcatt cagcggatag agaagcgggt
     4081 tgcaaaagac aagtacatca ataccagtga attaaactcg ctacttcaac aagaaataga
     4141 agcaatactg gcggatgccg gggatcaggg ctaccgggat ttcaccattc cctcaggaca
     4201 aaaaccgtat gtcatcctgg tcgtcggggt caacggtgtc ggcaaaacga ccaccatcgg
     4261 caagctggct tacaatttca aaagggccgg gcggtccgtt attcttgggg cggccgatac
     4321 ctttcgcgcc gctgcggtgg accagctgac catctggagc gagcgggcag gggtgggtat
     4381 cgtcaaacag gggatgggct ccgatccggg cgccgtggcc ttcgataccg tacaaagcgg
     4441 ggtggccagg ggtaccgatg tcgtcattat cgatacggca ggccggctac ataacaaggc
     4501 ctacctgatg gaagagttga gcaagatcaa tcgcgtgatc aaaaaggtga tccccgacgc
     4561 tcctcatgaa gtgctgctgg tattggacgg ttcaacaggt cagaatgcga tcgaacaggc
     4621 ccggcagttt acggctgcca cggatgtcac tgcgctcgcc attaccaagt tggatgggac
     4681 ggcaaaaggc ggagtggtgc tggctatcgc caaccaattc aaaatacctg taaaatttat
     4741 cggagtggga gagaagatgg aggatctgct tgtctttgac aaacatgagt tcgtcgatag
     4801 tctgtttaat ttagaaggcc agcgatagct ggccttctgt aatatttacc aggtatactt
     4861 cagtcccaat cctccccata cgccgagata aacatgattc agctccagat agggattgat
     4921 atcgaggctc gccgcaatgg gcgcgccgct gaacttgtat tccagaccca gcacgccgtc
     4981 aacgccgata gagacggtgc cgctgttgta ataataatca ttgtaaccat aaccgttgaa
     5041 gacgccgaaa tgcgcgcccg gcccgacata ccatttcaga ccgtcggctc cgctgatatc
     5101 gccatggatc tcatacagac cggtcaaacg aaaacctccg tcccagaaat tgagcaggcc
     5161 ttcgagggcg ttgtcatccg aaacgaaatg tttgatggaa atggcgcccg gccagaactt
     5221 aatgccgatg gcggtggaat aactgtttcc ttcggcctgg gcaaagccgc gggttgaaaa
     5281 aataaaggca aaaatgcttg ctaataacag ggtttttctc atattctgat ttgctttgtg
     5341 tggcaaatat ataaaaaacc atgccataat tgcggataca tgtaatttac gttcaactag
     5401 ttaagcatca ataaggcaaa ctggcccaga gttcgtaact ttgcggccca cacattatga
     5461 agacgaggtc attacagaag gataaggtga atatcatcac cctgggttgc agcaagaata
     5521 tggtagatag cgaggtcctg agcgggcaat tgcgcgccaa tgccatcgac gtcgtccacg
     5581 agaattccaa aaaagaccac aatatcgtcg tcgtcaacac ctgcgggttt atcgacaagg
     5641 cgaaggaaga atccatcaac accatcctcc gtcaggtgga cctgaaaaag aaaggtcgtc
     5701 tcgacaaggt gtatgtcacc ggctgtctga gccaacggta tcgcgaggac ctcgaaaaag
     5761 agatacccga ggtggatgcc tggttcggga ctatggagct gcctttgctc ctgcgccgga
     5821 tggacgccga ttacaaatcc gagctgttgg gcgagcgtat gctggccact ccgcaacatt
     5881 acgcttatct caagatatcg gaaggatgta accgcacctg cgctttttgt gcgatccctt
     5941 tgatgcgcgg cgggcatgtc agccggccga tggatgatct cgtacgcgaa gcggaaggac
     6001 tggtcagacg cggcgtaaag gagatcctgc ttatcgcgca ggaacttacc tactatggtc
     6061 tcgatctcta caaacaacgg atgcttcctc agctgctgca ccggct
//
LOCUS       DASYSV010000112        10968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1430020, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000112 DASYSV010000000
VERSION     DASYSV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430020"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1998)
                     /locus_tag="VGQ51_03610"
     CDS             complement(<1..1998)
                     /locus_tag="VGQ51_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035676.1"
                     /translation="MKSFFIDIFRRNIYLLLTAAGLFISGYLLNLYFSSDASVGFLRG
                     NIQSFLRDRQVDFDKVTRDTALLSRLAGQSYSPPQLKSFIDRKYGIFLYTLDSIGEPA
                     TLRFWNDQRSEPTSEMLYGPDSSAFLDLPNGEYEYVRRNVTTARKEHMIAIALIPVRW
                     QYYISNPNLTPEFVDIPSAEERVMIVSNPTEFPVRDNSGRVIFYLQKRPGYHAPAHNW
                     PIPMVILLATLLLLIIIHNIAHSIRERWGPALGIGFLITVILLMRAMIYISPEMLNLR
                     QFELFDPRIYGSSVILSSLGDLVINAFLFCWIILFIRRELSDYTIPQSIQRWKNWTWI
                     VTGLTLLIVTTFEFANIVQSLVTDAKISFNVTNFFSLLDNYSFVGFIALAALALGYFF
                     LSQVLFQLIGYLIRSLGIVLYILTATIGLLLLTFIHGTETVELDIFVLAWLLAYVWLM
                     QRRIFFDLRFRLNVSEVLFWLFIFSTSISAVILFENRKIEEGERRRMAERLADQANPE
                     SEKMLSIVFGYIDNDFLASNFARFRHFEENAAIKDSMVNRSLSMYQTKFDTRIYTFDG
                     NEKPLFNDPPLSFDTLNTIFTVAARNTDLPDLKYYLRSFDKYTYIYRKTLRNDSTGKM
                     AGYLFILAEPKEYKGTDALVPELFQQKTASLPEYYAIYNKRE"
     gene            complement(2033..2836)
                     /gene="mazG"
                     /locus_tag="VGQ51_03615"
     CDS             complement(2033..2836)
                     /gene="mazG"
                     /locus_tag="VGQ51_03615"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEV9035677.1"
                     /translation="MNENQKAFQRLVEIMDDLREKCPWDRKQTIATLRPLTIEELYEL
                     ADAVTEEDWKGIKEELGDLLLHIVFYARIGKEQGKFTLDEMINGICDKLISRHPHIYG
                     DTNSHPGLVEVRNEEDVKRNWEQLKLREGKKSTLSGVPVSLPATVKATRLQEKAGKVG
                     FEWEQTEQVWEKVKEEMDELQEAVASRDPDKIAEEFGDLSFSLINYARFLQIDAENSL
                     ERTNKKFIDRFTRMEEVARSRGLSLADMSLAEMDEIWNDIKKAGSAPKQ"
     gene            complement(2866..4101)
                     /locus_tag="VGQ51_03620"
     CDS             complement(2866..4101)
                     /locus_tag="VGQ51_03620"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEV9035678.1"
                     /translation="MSHLKERKEKNCLNCQAQLVGRYCHVCGQENLEPRETVWHLVSH
                     FFNDITHFDGKFFATVKYLLRKPGFLSHEYSKGRRMAYLNPIRMYVFTSAIFFIILYS
                     LTRPGDMMHVTTTKDKPVGIAELQAQEARRQKRLASADDADDREELRSNIQKGAIEIA
                     AIKKTYGDTTTRKFTEDELTGILVQAYIDSASVAEGLRSADSTRFKNAVKHISNPIGT
                     TNIFGWSGDYTSVEDYDSVQRALPDSARDGWFKHLMMRRGVVIAQEWHEDSRLFKEHL
                     GENVLHSFPKILFVSLPIFALILNMLYFRHKQYYYVNHGIFTIHVYCATFILMLAGML
                     INQLDKALGVHWVHVVCDLLVFGIFLYAMIYLYKAMRGFYKQGRGKTFLKYFITFVTA
                     SVINLILLFIFLLITLFSV"
     gene            4190..5041
                     /locus_tag="VGQ51_03625"
     CDS             4190..5041
                     /locus_tag="VGQ51_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HEV9035679.1"
                     /translation="MIKLPIGCLLLCVITQASKAQYFYKDLIATRENTAHWQSYRDNH
                     VKSITLSSFEADGKPTEGFVGEKQLTDDCSEITTHTKTSGTTDSWIITDYSSQGRLLK
                     NTDTSDTYRNVTVYQYDDRGRIRAITDTSIETDNQLKDVEMHLWTYDATHPDKPSSMI
                     KVFNGVDTTYVRFVLDEKGNVAEEHASRHGSSMPVIYYYYDASSRLTDIVRYSMKAQR
                     LLPVNMFEYAENGKMESMLVVPEEGNSFYQKWYYEYNEKGLKVKDFCYNKQKELLGSV
                     DYEYGYK"
     gene            5047..5682
                     /locus_tag="VGQ51_03630"
     CDS             5047..5682
                     /locus_tag="VGQ51_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035680.1"
                     /translation="MRIFLCLCLSMGALSVCSQDLDYPDFRQKKDNFSKIHEQDIRAD
                     VASFALAAVDESIGKPQLPSLPVVDYGSNFMKYANDNIQVIIRTGFFLPAKHKLMVQE
                     GHLLRIDGKPYYGGVYGQNPHTTIESITVVVGTDTVPIPSTAYFDLYDPKFSFRENGA
                     TRSRNGVFLSGDKHTFYIYMLNIDNKGTEYTWVIRDKQYLRRVVDFGFLEN"
     gene            5745..7280
                     /locus_tag="VGQ51_03635"
     CDS             5745..7280
                     /locus_tag="VGQ51_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9035681.1"
                     /translation="MASVSIRTLAGKWIMTSAILASSMAFIDSTALNVVLPSLQKSLH
                     ATGADLFWILNAYLLMLASLILVGGSLGDRLGRKKVFMTGIAVFVTGSAACGLAPHTG
                     QLILFRVLQGLGGALMIPGSLALISSSIEEKEKGKAIGTWSAITTVVTMGGPILGGTL
                     ADAGLWRYIFFINVPIGIATLLVLWRKVEEPPIEKAADSGIDIPGAISIALGLAALTF
                     GFLRIPALGFHHWIVRGSLIVGLVLLVLFVITERRSKHPMMPLSLFANPVFAGVNGLS
                     FFLYAGLGAGMLFLSLNFIQMQGYNQLESGMTFLPFTILMVLLARPAGTLSDRIGPRI
                     FLTVGPATAGAGLLLLSLIGQTRGPTHYWTTYFPGVLVLGLGMSLTVAPLTTTVMRSV
                     SDIQSGLASGVNNAMTRIANVFANAIFGALAILFFTGTLHQRIDRWGLAPATKEIVLA
                     QTTDLGNAHVPDTVPKKDRPAVAAAYRDGFISTYQKILRLAACLAFIGAAIGFISVRK
                     PAT"
     gene            7399..8052
                     /locus_tag="VGQ51_03640"
     CDS             7399..8052
                     /locus_tag="VGQ51_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035682.1"
                     /translation="MKIGILCLLSAITLHAPAYAQLPVAAEKHHRVLLQNDYIRILDG
                     HIPARDTTPVHLHAANSVVVFLSLSTFAIQKPGEPPVVTHVRPGDLKYAGYGDKPVNH
                     IVWNESRNVFHFYVVELVKTHAAGAPCPISPQPGLMLQWKQPSVEAYYLEPPAGGSCR
                     LPATSCARLLIGLSGTTTASTPARTEIRQPEGYVYFPPGTRIDIRGDHSRCILLEIR"
     gene            8164..10029
                     /locus_tag="VGQ51_03645"
     CDS             8164..10029
                     /locus_tag="VGQ51_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV9035683.1"
                     /translation="MRLQVLIAATISLLFLVEPGRAQKPNDSAALAEAHATFRLISQY
                     PDSALKLSETQLASAQKGGDQALAAVAYHTRGWAWLHKGDYSKSFPDLKKAAQLFHEL
                     HNPMDEMHVYINLGLAYSQHSEFANSVRYLILADSLAQKLNSEPTKAEVHRQMGILYR
                     EQGQYAMAIPHFRQSMSMYRQLHDTIHFFGAASSLSAVYMGMDKPDSSLALLQDCLPL
                     VGALRGYEKGMLMEHYGDAYFALSHYDKAMDSYGGAYRLFLANHNDADMAYEAMNVGK
                     TLTRMRNYRDAETYLLLSYRINDSLKMTNYTHDVAEQLSNLFEAKGDWHKAYHWLAIK
                     DSLQDFLDLRAVNEKTAQLQAQYEADKKEKEISLLKEEKALNLAILERQRVFQRGAIV
                     VVILLVLIGLLLIGRYRAASRARQLIEMEKMRNHIARDLHDDMGSALSSIHIISRVAL
                     TNSSDGPKMTDRMKKIHEHSGQILENMSDIVWTINPANDTLEKVIFKMREFAADILDP
                     LNIEYVFQQEGDFQGTRLGLQTRRDLYLIFKEAVNNAAKYSGCSKVVITVAVNDRQVE
                     VNIGDDGIGFDKEEVKFGNGLKNMEERASQIGGNLSIATAPGKGTTITLRVRSHD"
     gene            10075..10713
                     /locus_tag="VGQ51_03650"
     CDS             10075..10713
                     /locus_tag="VGQ51_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9035684.1"
                     /translation="MQTGIVIFEDNQSLRESIAQLLADSNSFVLLGSFPNALQADRHV
                     RDLKPEVILMDIDMPGGVSGIDAVKKIRTFDRQTPIIMLTVFDDQTNVLEAVFAGASG
                     YLLKKHLSDHLIDSIREALSGGAPMSPNVARMVIASMHKYPSSAGNDYQLTTRETEIL
                     SALAQGTGYKAISARLFISVDTVRSHIKNIYQKMQVHSQLEAVARGRDAGII"
     gene            complement(10752..10837)
                     /locus_tag="VGQ51_03655"
     tRNA            complement(10752..10837)
                     /locus_tag="VGQ51_03655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10801..10803),aa:Leu,seq:taa)
BASE COUNT         2561 a         3036 c         2874 g         2497 t
ORIGIN      
        1 ctcgcgtttg ttataaatgg cataatattc cggaagcgag gcggtctttt gctgaaacag
       61 ttcgggcacc agcgcatcgg tacccttata ctccttcggc tcggccagga tgaacaggta
      121 gcccgccatt tttccggtag aatcgttgcg cagcgtcttc cggtagatgt aggtgtattt
      181 gtcgaaggac cgcagatagt atttgagatc gggcagatcg gtgttcctgg cggcgaccgt
      241 aaaaatcgta ttcagggtat cgaaggacaa gggaggatcg ttaaacaacg gcttctcgtt
      301 cccgtcaaaa gtatagatac gggtgtcgaa cttcgtctgg tacatagaaa ggctgcggtt
      361 gaccatactg tccttgatcg ccgcattctc ctcaaaatgc ctgaaccggg cgaaattgga
      421 tgccaggaaa tcgttgtcta tatacccgaa aacgatactg agcatctttt cgctttccgg
      481 gttagcctgg tcggccagcc gctcggccat gcgtctccgc tcgccttctt ctatcttccg
      541 gttctcgaac aggatgacgg ccgatatgga cgttgaaaag atgaacagcc agaacagcac
      601 ctcggagacg tttagccgga accgaaggtc gaaaaagatg cgccgctgca tgagccagac
      661 ataggccaac agccaggcca gaacgaaaat gtccagctcg acggtttcgg tcccgtggat
      721 gaaggtcagc aggagtagtc cgatggtggc cgtcagaatg tacaggacga tacccaggct
      781 gcggatcagg taaccgatca gctggaaaag cacctgcgac aggaagaaat agcccagcgc
      841 cagcgccgcc agcgcgatga accctacaaa gctgtagttg tccaaaaggc tgaagaagtt
      901 ggtgacgttg aacgatattt tcgcgtccgt gaccagcgac tggacgatat tggcgaattc
      961 gaaggtggtg acgatcagta atgtcagccc ggtgacgatc caggtccagt tcttccatcg
     1021 ctggatggac tggggaatgg tataatcgct aagctcgcgg cggataaaga ggatgatcca
     1081 gcagaacagg aaggcgttaa tgacgaggtc tcccagcgag ctcaggatga cgctcgatcc
     1141 atagatccgg ggatcgaaca gctcgaattg ccggagattg agcatttccg gggagatata
     1201 gatcatggcg cgcatcagca ggatgacggt gataaggaac ccgatgccca gtgccggccc
     1261 ccatctttcc cggatggaat gtgcaatatt atggatgatg atgagtagca ggagtgtggc
     1321 cagcaggatc accatgggga tcggccagtt atgcgcggga gcgtgatacc ccggtctttt
     1381 ctgcagatag aatatgaccc ttccggagtt gtcccggaca gggaattcgg tcggattgga
     1441 gacgatcatg acgcgctctt cggcagaggg gatgtcgacg aattcaggcg tcagattggg
     1501 attggaaata taatactgcc agcgcacagg gatcagcgct atggcgatca tatgttcctt
     1561 tctggcggtc gtcacgttac ggcggacata ttcgtattca ccgtttggga ggtccaggaa
     1621 ggcgctgctg tccggcccgt aaagcatttc ggaggtgggt tccgaccgct gatcgttcca
     1681 gaaacgcagg gtcgccggtt cgccgatgga gtccagcgta tacaggaaga taccgtactt
     1741 cctgtcgata aaggatttca gctgcggcgg tgaataggac tgacccgcga gacgggaaag
     1801 caatgcggta tcccgggtca ccttatcgaa atcgacctgt ctgtcccgga gaaaggactg
     1861 gatattgccc cggaggaagc cgaccgacgc atcgctgcta aaataaagat tgagcaggta
     1921 cccgctgatg aataggcctg cagcggttag caggaggtag atgttcctgc gaaaaatatc
     1981 gataaaaaag gacttcaatc gcctccttcg ttttttttat tgtttaggcg cgttattgtt
     2041 taggcgcgga tcccgccttc tttatgtcgt tccagatctc atccatctcc gccaggctca
     2101 tatcggccag cgacaggccc ctggagcggg ccacttcctc catgcgggta aatcgatcga
     2161 tgaatttctt attcgtacgt tccagggaat tctccgcgtc tatctgcaaa aatcgtgcat
     2221 aattgatcaa agaaaagcta agatcgccga attcttcggc tattttatcg ggatcgcggc
     2281 tggcgacggc ttcctgcagc tcgtccatct cctcttttac cttctcccat acctgctcgg
     2341 tctgctccca ttcgaaacct actttcccgg ctttttcctg cagccgggtt gcctttacgg
     2401 tggcgggcaa cgatacgggc acgccgctca gggtggattt cttaccctcc ctgagcttta
     2461 gctgctccca gttgcgtttg acatcctctt catttctgac ctctaccagc cccgggtggc
     2521 tgttagtgtc gccatagata tggggatgcc ggctgataag tttgtcacaa atgccgttga
     2581 tcatctcgtc gagcgtgaat tttccctgct ccttcccgat ccgcgcataa aaaacgatgt
     2641 gaagcaacag gtcccccaat tcctctttta tacccttcca gtcctcctcc gtgacggcat
     2701 ctgccagctc atatagctcc tcgatagtta gcggacgcag ggttgcaatg gtctgtttcc
     2761 ggtcccaggg acatttctca cgcagatcgt ccatgatctc caccaatcgc tggaaggcct
     2821 tctgattctc gttcattttt caactcattt ttgccgtatc tgccgctata ccgaaaagag
     2881 ggtgatgagc aggaagatga acagcaggat caaattaata acactcgcgg taacaaaggt
     2941 gatgaaatat ttcagaaaag tcttacccct cccctgttta taaaagccgc gcatcgcctt
     3001 atacaggtag atcatcgcat agagaaaaat tccgaagaca agcaggtcgc agaccacatg
     3061 gacccaatgt acgccgagcg ccttgtccaa ttgattgatg agcatgcctg ccagcatcag
     3121 gataaaggtc gcgcaataaa catggatcgt gaagatgcca tggttcacat aataatactg
     3181 cttatgacgg aaatagagca tgttcaggat gagcgcgaat atcggcaggg aaacgaacag
     3241 tatcttgggg aaggaatgaa ggacgttctc acccaaatgt tctttgaaga gacgcgagtc
     3301 ctcgtgccat tcctgggcga tgaccacccc cctgcgcatc atgaggtgtt tgaaccagcc
     3361 gtcgcgcgca ctgtcgggta acgcgcgttg tacggagtca tagtcttcca cgctggtata
     3421 gtcaccgctc catccgaaga tattggtagt cccgatcgga ttggagatat gtttgacggc
     3481 atttttaaat cgggtgctat cggcagacct gagcccttcg gcgactgatg ccgagtcgat
     3541 ataagcctga acgagtatgc cggtcaattc gtcttcggta aatttccgcg tggtcgtatc
     3601 cccataggtc tttttgatcg cggcgatctc gatggcgcct ttttgaatat tgctgcgcag
     3661 ctcttcccgg tcatcggcat catccgcaga ggccagcctt ttctgccggc gagcctcctg
     3721 tgcctgcaat tcggcgatcc ctacgggctt atcctttgtc gtggtgacgt gcatcatatc
     3781 tcccggcctg gtcagggaat acaggataat gaaaaaaatg gccgaggtga acacatacat
     3841 ccggatcgga ttcaaataag ccatccgtct ccccttcgaa tattcgtggg aaaggaatcc
     3901 cggcttgcga agcagatatt tgacggtagc gaagaatttc ccgtcgaaat gggtgatatc
     3961 gttgaagaaa tgggatacca ggtgccagac cgtctccctg ggttccaggt tctcctggcc
     4021 gcagacatga cagtatcggc cgaccagctg ggcctggcaa ttcaggcaat ttttctcctt
     4081 acgctccttg agatgggaca cagcagttaa aatttcacct aaaataagcc gtccggggca
     4141 gtttcgtgta ttaaaaaaag gattttttcg ttcctttgat atgattgata tgatcaagct
     4201 acctatcgga tgcctgctgt tgtgcgtaat tacgcaggca agtaaagccc aatatttcta
     4261 caaggacctg atcgccaccc gggagaacac cgcccactgg caatcatacc gggataatca
     4321 tgtcaaatcg attaccctga gcagctttga ggccgacggc aaacccactg agggattcgt
     4381 aggcgaaaag cagctgaccg acgactgttc tgagatcacc acccatacca aaacgtcggg
     4441 cacgaccgac tcctggatca tcaccgatta ttcatcccag ggacgcctcc tgaaaaacac
     4501 cgataccagc gatacctacc gcaacgtaac cgtctatcag tatgatgacc gcgggcgcat
     4561 ccgggcgatc acggatactt ccatagaaac ggacaatcag ctgaaggatg tcgagatgca
     4621 tctctggact tatgatgcca cgcacccgga caagccttcg tcgatgataa aggtcttcaa
     4681 cggtgtcgac accacctatg tacggttcgt actggatgaa aagggcaatg tggccgaaga
     4741 gcatgccagc cgtcatggct cttccatgcc ggtgatctat tactactacg atgcgtccag
     4801 tcgccttacg gatatcgtcc ggtatagcat gaaggcacag cggctgctgc cggtgaatat
     4861 gttcgaatat gcggaaaacg ggaagatgga gtccatgctg gtcgtccccg aggagggaaa
     4921 cagcttctac cagaaatggt attacgaata caatgaaaaa ggcttgaaag taaaagactt
     4981 ttgttataac aaacagaaag agttgctggg aagcgtcgat tacgagtacg gctataaatg
     5041 atcactatgc gtatattcct ttgcctgtgt ctgtcaatgg gcgcgttatc agtttgttcg
     5101 caggatttgg attacccgga ttttcgtcaa aaaaaggata atttctccaa aatacacgag
     5161 caggatatcc gggcggatgt cgccagcttc gcgctcgccg cggtggatga aagcatcggg
     5221 aaaccgcaat tgccgtcgct gccggtggta gactacggtt cgaatttcat gaaatatgcg
     5281 aatgacaata tccaggtcat catccggact ggcttctttc tgcccgccaa acacaagctc
     5341 atggtgcagg aaggtcatct gttgcgcatc gacggcaaac cctattacgg cggcgtctat
     5401 ggtcagaacc cgcataccac catcgaatcg ataaccgttg tggtaggtac ggataccgtc
     5461 cccatccctt ccactgcata tttcgatctc tacgatccca agttcagctt ccgtgagaat
     5521 ggcgctacca ggtcccgaaa cggcgtattc ctgtcgggcg acaagcacac cttctatatc
     5581 tacatgctca atatcgacaa taaaggcacc gaatacacct gggtcatccg cgacaaacag
     5641 tacctgagac gggtagtcga ttttggattc ctcgagaatt gatggagcgc cccgacccat
     5701 gacgcccccg ccgctgaacc accccctttc gttatcttta ggtcatggca tcggtatcca
     5761 tccgaacact cgcgggcaaa tggatcatga cttctgcgat cctggcctcc tccatggcct
     5821 tcatcgactc gaccgcgctc aacgtcgtgc tgccatcgct gcaaaaaagc cttcatgcca
     5881 ccggcgccga tctattctgg atattgaatg cctacctgct gatgctggcc tcgctgatcc
     5941 tcgtcggcgg ctcgctggga gaccggctgg gccggaaaaa ggtattcatg actggtatcg
     6001 ccgtctttgt tacgggaagc gctgcctgcg gcctggcgcc gcatacgggt caactcatcc
     6061 tattccgggt cctgcagggc ctgggtgggg cattgatgat ccccggtagt ctcgcgctga
     6121 tctcttcttc catcgaagaa aaagaaaaag gtaaggccat cggcacctgg tccgccataa
     6181 caaccgtagt caccatggga gggcctatcc tcggcgggac gctcgcggat gccgggctat
     6241 ggcggtatat cttctttatc aacgtcccca tcggtatcgc aacgctgctg gtcctctggc
     6301 ggaaggtaga ggagcccccc atcgaaaaag ccgctgattc cggcatcgac atccccggag
     6361 ccatatccat agccctcgga ctcgcggcac tcaccttcgg ctttctccgc atcccggcgc
     6421 tgggtttcca tcattggata gtccgcgggt ccctgatcgt ggggttggtc ctgctggtcc
     6481 tgttcgttat caccgagcgc cgcagcaaac atccgatgat gcccttatcg cttttcgcca
     6541 atcccgtttt tgccggcgtc aacggactgt ccttttttct ctatgccgga ttgggcgccg
     6601 gcatgctttt tctttcgctc aacttcattc agatgcaagg ttataaccag ctggagtccg
     6661 ggatgacctt cctgccgttt actattttga tggttttgct ggcgcgaccg gcaggaaccc
     6721 tctccgatcg catcgggccc cgaatattcc ttacggtggg tccggcgacg gccggcgccg
     6781 gtttactgct gctttccctg ataggacaaa cccggggacc gacccattac tggacaacct
     6841 attttcccgg tgtactggtc ctcggacttg gaatgtccct taccgttgca ccactgacga
     6901 cgacggtcat gcgctcggtc agcgacatac aatcaggact ggcctccggc gtcaacaacg
     6961 ccatgaccag gatcgcgaat gttttcgcca atgccatctt cggcgcactg gccatattgt
     7021 tcttcaccgg gacgcttcat cagcggatcg acagatgggg attagccccc gcaacaaaag
     7081 agatcgttct cgcgcagacc accgacctcg gcaatgctca tgtaccggac acagttccca
     7141 aaaaggatcg accggcggtg gcagccgcct atcgcgacgg attcataagc acctaccaaa
     7201 agatccttcg attggcagca tgcctcgcat tcataggggc ggcgataggg tttatatccg
     7261 tgagaaagcc tgcgacgtga aaggctttat aaccagcccg cattgccgcc gtgaccccgg
     7321 cgtttaccgg catagttcag ccgggatggt agcatttgtc atcccggtcg ccttatttta
     7381 catggctaaa gcatttgtat gaagatcggc atcctctgtc tactgtccgc gatcaccttg
     7441 catgcccccg cctatgccca gctgcccgtc gccgcggaaa agcaccatcg cgtcctgctg
     7501 cagaacgact atatccgcat ccttgacgga catatccccg cccgcgatac gacccccgtg
     7561 catctgcatg cggcgaattc cgtggtggta tttctttcac tgtcaacttt cgcgatccaa
     7621 aagcccggcg agccgccggt ggtcacccat gtgcgcccgg gagacctgaa gtatgccggt
     7681 tatggcgaca aacccgtcaa ccacatcgtc tggaacgaga gcaggaacgt tttccatttt
     7741 tatgtggtcg agctggtgaa gacccatgcc gccggcgccc cctgccctat ctccccgcaa
     7801 cccggtctaa tgctccaatg gaaacaaccg tcagtcgaag cctactacct cgagcctccc
     7861 gccggcgggt cctgccggct ccctgccacc tcctgcgccc gtctcctcat tggcctgtcg
     7921 ggtacgacta ccgcctcgac tccggcccgc acggagatcc gtcaacccga gggttatgtc
     7981 tattttcctc ccggcacccg catcgatatc cggggcgacc attcccgttg tatcctgctg
     8041 gaaatacggt aactctgccg cgcgccccgc cccgctgctc catcgcgtga tcccgccccg
     8101 gcgattttac cgccttaccc cacgatttac cttatcttgt gtaggctaat tttcctccct
     8161 cccatgcgcc tacaggttct gattgccgcg acgataagtc tcctcttcct tgtcgaaccg
     8221 ggcagggcgc agaaacccaa tgactccgca gcgctggctg aagcgcacgc tactttccgc
     8281 ctcatttcgc aataccctga ctcggcgctg aaactttccg aaacacagct ggcatcggct
     8341 caaaaagggg gagaccaggc gctggctgcc gtagcttatc acacacgggg atgggcctgg
     8401 ctgcacaagg gagactatag caagagcttc cccgatctaa aaaaggccgc acagttattc
     8461 cacgagctgc acaacccgat ggacgaaatg catgtgtaca tcaacctggg acttgcctac
     8521 tcccaacaca gcgagttcgc caacagcgtt cgatacctta tcctggctga ttcgctcgca
     8581 caaaaattga atagcgagcc taccaaggcc gaagtgcacc gtcagatggg tattctctat
     8641 cgagaacagg ggcaatatgc catggccatt cctcatttca ggcagagtat gtcgatgtac
     8701 cggcagcttc atgataccat ccattttttc ggtgccgcct cgagcctcag cgccgtctat
     8761 atgggtatgg acaagccgga cagcagtctc gcgttgctgc aggattgcct gccgctggtc
     8821 ggggccctgc gcgggtatga aaaaggaatg ctgatggaac actatgggga tgcgtacttc
     8881 gcgctttccc actatgacaa ggccatggac agctatggcg gggcataccg gcttttcctc
     8941 gccaaccaca acgacgccga tatggcctat gaggcgatga acgtaggtaa gacgcttacg
     9001 cgtatgcgga actaccggga tgcggagacc taccttttat tgagctatag gattaatgac
     9061 agcctcaaga tgaccaacta tacccatgac gtggccgaac agctgtccaa tcttttcgag
     9121 gcgaaaggag actggcataa ggcctatcac tggctggcca tcaaagactc tttgcaggac
     9181 tttctcgatc tgagggcggt gaatgagaag acggcgcaac tgcaggcgca gtacgaggcg
     9241 gataagaagg aaaaggagat atcgctgctg aaggaagaaa aagctctcaa tctcgcgata
     9301 ctcgaacggc aacgggtgtt ccagcgcgga gcgatcgtag tggtcatcct gctggtcctg
     9361 atcggcctgc tgctcatcgg gaggtaccgg gccgcctcca gggcaagaca gctgatcgag
     9421 atggagaaga tgcgaaatca tatcgcgcgc gatcttcacg acgacatggg ttcggcgctg
     9481 tccagcatcc acatcatcag cagggtggcc ctgaccaata gcagcgacgg tcccaaaatg
     9541 accgatcgta tgaaaaagat ccatgaacac tccggacaga tccttgaaaa catgagcgac
     9601 atcgtatgga cgatcaaccc tgccaacgac acgctcgaga aggtgatctt caaaatgcgc
     9661 gagttcgccg ccgatatcct cgacccgctg aatatcgaat acgtctttca acaggaaggc
     9721 gatttccagg ggaccaggct ggggctgcag acccggagag atctctatct catcttcaag
     9781 gaagccgtta acaatgcggc gaaatatagc gggtgttcga aagtggtgat caccgttgcc
     9841 gtaaacgacc ggcaggtgga agtgaatatc ggcgatgacg gcatcggttt cgacaaggaa
     9901 gaagtgaaat tcggcaacgg tctgaagaat atggaagagc gtgcaagcca gatcggcggc
     9961 aatctcagca ttgctactgc acccggcaaa ggcacgacta taacccttcg ggtaagatca
    10021 catgattgag ggatgcatcg cggttttttt ttcccgaatt tgtaaaagga aatcatgcag
    10081 acaggcatag tcatattcga ggacaaccag tccctccgcg aaagcatcgc acaattgctg
    10141 gccgattcaa attcattcgt tttgctgggt agtttcccca atgccctgca agcggatcga
    10201 cacgtcagag acctgaagcc ggaagtcatc ctcatggata tcgatatgcc cggcggggtc
    10261 agcggcatcg acgccgttaa aaagatccgg accttcgatc gtcagacgcc gataatcatg
    10321 ctgactgtgt tcgacgacca gacgaatgtg ctggaggccg tattcgcggg agcctccggt
    10381 tatctcctca agaaacatct ttccgaccat ctcatcgact ccatccgcga ggcattatcc
    10441 ggcggcgcgc cgatgagccc aaatgtcgcg cgtatggtca ttgccagcat gcataaatat
    10501 ccttcctccg ccggcaacga ttaccagctg acgacccgcg aaaccgagat cctttcggcg
    10561 cttgcgcagg gcaccggtta taaggctatt tccgcgcgtt tgttcatctc cgtggacact
    10621 gtccgcagtc atatcaagaa tatctatcag aaaatgcagg ttcattcgca gctggaagcc
    10681 gtcgcccgcg gccgcgacgc gggaatcatc taaaacaaaa aaggccctca ttctcatgat
    10741 gaccttcgaa gtacccggga cgggaatcga acccgtactg ccgttgcggg caacaggatt
    10801 ttaagtcctg cgcgtctacc tgttccgcca cccgggtgca tcggcttact aaagatataa
    10861 ataaaaaatc catcagcgtg atgcggatgg attttcgacc ataattcact catcagactt
    10921 cgtccgatct ggagcggaag acgaggctcg aacccgcgac ctcaacct
//
LOCUS       DASYSV010000113         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_920270, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000113 DASYSV010000000
VERSION     DASYSV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920270"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VGQ51_03660"
     CDS             <1..624
                     /locus_tag="VGQ51_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV9035685.1"
                     /translation="TGHQFWWEVNYPGKHVTTANEVHLPVGRQLVMEMRSADVVHDWW
                     VPQLGNKMDLVPGVKNYLWLNIHKPGDYIGTCSEFCGEQHAWMRIRVVAQDSLDFNRW
                     LDSNAVAASPPRDDLARQGKDLFEVKSCANCHRINGTSAAGTAGPDLTHVGSRSTILT
                     GMLETSEKNLTEWIEHPQEIKHGAYMPEFLFAKDSIRALAHYLEQLK"
     gene            648..2327
                     /gene="ctaD"
                     /locus_tag="VGQ51_03665"
     CDS             648..2327
                     /gene="ctaD"
                     /locus_tag="VGQ51_03665"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEV9035686.1"
                     /translation="MGTTIKIPDPASPFPGLGVETDQGLLQWLSAVDHKIIGIMYMVM
                     SLFFLVVGGVLVLLMRIQLAVPMNHFLPPETFNQFFTMHGTTMIFFVAMPAIFGFAVY
                     FIPLMIGANEMAFPRLNAFGFWMTLFGGLLLYFSYLAGGAPDAGWFNYAPLSERNYSF
                     HSGVDYYCMGLLVSGIGSVVTSVNIIVTVLHYRIPGMRLNKLPLFVWMVFVNSFLLLA
                     AFPSLNAALAMLLLDRQLNAHFFNPTSGGSALLWQHLFWIFGHPEVYIVILPAFGMLS
                     EVFQVFSRKKIFGYPFVAASSVAIALLSFGVWAHHMFAVGMGNTLNNFFSAGSMLIGI
                     PTGVKIFNWLGTMYGGKIRFTTSMLFAIAFLIEFTIGGLTGIAFAIFPIDWQLTDTYY
                     VVAHFHYVLIGGTVSGLLAALFYWFPKMTGRLIDERGGKWFFWLWTIGFNLTFLIQHV
                     LGILGMPRRVYTYPRLPWYGTLNFISTVGAMIMGIAGLLLVCLIWKCGRHGRPSGPDP
                     WDAYTLEWATRTSPPPLKNFEDLPPVTSRRAWWDMKQSRRAADPEQQNSES"
     gene            2324..2878
                     /locus_tag="VGQ51_03670"
     CDS             2324..2878
                     /locus_tag="VGQ51_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEV9035687.1"
                     /translation="MTQSEDTTMMQAVIFTEAFFFLCLIMAFVYMAYNTGFEPHELSR
                     LDIRTAGIFTAVLIASSFTLHAAERGYARGKPVVLKTWLIVTIVLGAIFLAGQGHEYI
                     SLIRENVTLNGSVFGASFFTLTGFHGLHVLVGLLILSIVLALAFLRDSKSAIGTVAMY
                     WHFVDIVWIFVFTVVYVLPQFTNI"
     gene            2893..3624
                     /locus_tag="VGQ51_03675"
     CDS             2893..3624
                     /locus_tag="VGQ51_03675"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEV9035688.1"
                     /translation="MRILSLLLGILVLAICLGIPLPALDANSPFSSHMLRHTSLMLLA
                     APLMALAISPAGFAKKQLTAMSRLTNRLPFAAWLTGVAVMWIWHVPGWYNSITPGADT
                     ISCAPLAPALFSHLSSIAPFSRPLIIADRVFPLLNDLSLLIAGFIFWWPVITPCHSFR
                     LSPLRGVAYLASACICCSLLGLLITFARPGVYRGISLSDQQTGGMIMWIPCCLIYLTA
                     SMGLLIEWLSKKEADTSISIQNHHA"
BASE COUNT          720 a         1069 c          981 g          959 t
ORIGIN      
        1 accggccacc agttctggtg ggaggtgaat tatcccggca aacatgtcac cactgccaat
       61 gaggtccatc ttcccgtcgg ccggcaactg gtaatggaaa tgaggagcgc cgatgtggtc
      121 catgactggt gggtaccgca attagggaat aaaatggacc tcgttcccgg agtcaagaat
      181 tatctctggc tgaacatcca caaaccaggc gattatatcg gcacctgcag cgaattttgc
      241 ggcgagcaac atgcctggat gcggatacgg gtcgttgcgc aggattcgct ggacttcaac
      301 cgctggctgg attcgaatgc agtagccgct tcgcctccgc gggatgatct cgcccggcaa
      361 gggaaggatc tattcgaggt caagtcctgc gccaattgtc atcgtatcaa cggcacatcc
      421 gccgcgggta cggccggtcc cgatctgacg cacgtcggca gcaggtccac catcctgacg
      481 ggaatgctgg agacaagcga gaaaaatctt accgaatgga tcgaacatcc gcaggagatc
      541 aaacatggag cctatatgcc tgaattcctg ttcgcgaaag attctatccg tgcccttgcc
      601 cactatctcg aacaattgaa ataaatcggt caatctgaca atacaatatg gggacaacca
      661 ttaaaatacc cgatcctgcg tcgccatttc cgggtctcgg cgtagagacc gatcagggcc
      721 tgttgcaatg gctcagtgcg gtggatcata agatcatcgg catcatgtat atggtcatgt
      781 ccctgttctt cctggttgtc ggcggtgtgc tggtcctgct catgcgcatc cagctcgccg
      841 taccgatgaa tcatttcctg ccgcccgaaa cgttcaacca gttcttcacc atgcatggca
      901 cgacgatgat ctttttcgtg gccatgcctg cgatattcgg ttttgccgtt tattttatcc
      961 cgctcatgat cggagcgaat gaaatggcct ttccgcggtt gaacgcattt ggtttttgga
     1021 tgaccctttt cggcggtctg ctgctctatt tcagctatct cgccggcggc gccccagatg
     1081 cggggtggtt caattatgct ccgctcagcg agcgcaacta ttcgttccat tccggagtcg
     1141 actattactg tatgggtctt cttgtctcgg gcatcgggag cgtcgttact tccgtcaaca
     1201 tcatcgtcac cgtgctgcac taccggatac cggggatgcg gctgaacaag ctgccgttgt
     1261 tcgtatggat ggtcttcgtc aactcttttc tgctgctggc cgcttttccg tcgctgaatg
     1321 ccgcattggc catgctgctt ctcgaccgcc agctcaatgc gcatttcttc aatccgacga
     1381 gtggcgggtc ggccctgttg tggcagcatc tcttctggat cttcggacat cccgaggtct
     1441 atatcgtgat ccttccagcc ttcggcatgt tgtccgaggt cttccaggta ttctcccgga
     1501 aaaagatctt cggctatccc tttgttgcgg cttccagcgt agcgatcgcg ttgctttcat
     1561 tcggcgtatg ggcgcatcat atgttcgcgg tcggaatggg caataccctc aataactttt
     1621 tttccgccgg cagcatgctg atcgggatac ccaccggcgt caagatcttc aattggctgg
     1681 gaacgatgta tgggggcaag atcaggttca ccacttcgat gctttttgcc atcgccttcc
     1741 tgatcgaatt tacgatcggg ggactcaccg gcattgcatt cgcgatcttt cctatcgact
     1801 ggcagttgac cgacacctat tatgtggtgg ctcatttcca ttatgtgttg atcggcggca
     1861 ccgtctcggg tctgctggcg gcgttgttct actggttccc gaagatgacc ggccgtctta
     1921 tcgatgaaag agggggaaaa tggttcttct ggctttggac catcggcttc aacctcacct
     1981 ttctgatcca gcatgtgctt gggatcctcg gtatgcccag aagggtatat acctatcccc
     2041 ggcttccatg gtacgggacg ctgaatttca tctccaccgt cggagcgatg atcatgggca
     2101 tagcggggct gctgctcgtc tgtctgatct ggaaatgcgg acgtcatggc cggccttccg
     2161 gtccggaccc ctgggatgcg tatacgctgg aatgggcgac acgtacttcg ccgccgccgc
     2221 tgaagaattt tgaggacctg ccgccggtga ccagccgccg ggcctggtgg gacatgaaac
     2281 aatcccgccg tgccgcggat ccggaacaac aaaacagcga atcatgacac aatccgaaga
     2341 tactaccatg atgcaggcgg tcattttcac cgaagccttt tttttcctgt gtctcatcat
     2401 ggccttcgtc tacatggcgt acaacaccgg cttcgagccg cacgagctaa gccggctgga
     2461 tatcaggacg gccggaattt ttacggcggt gctcatcgca agcagcttca cccttcatgc
     2521 cgcggaaaga ggatatgcaa gaggaaaacc ggtcgtgctc aagacttggc tgatcgttac
     2581 gatcgttctc ggggccattt tcctcgccgg ccagggccac gaatacataa gtctgatccg
     2641 ggaaaatgtg accctcaatg gaagtgtctt cggagccagc ttttttacac tcaccgggtt
     2701 tcatgggctg catgtgctgg tgggtctcct catcctttcg atcgtcctgg cgctggcatt
     2761 tctgcgggat tcgaaaagcg ctatcggcac tgtcgccatg tactggcatt ttgtggatat
     2821 cgtttggatc ttcgtattta ccgtcgttta cgtattacct caattcacga atatctgatc
     2881 aacccgtctg ctatgcgtat actatcctta cttctcggca tcctggtact tgcgatctgt
     2941 ctgggaatac cgctgcccgc gctggatgcc aattcgccgt tcagctcgca tatgctgcgg
     3001 catacctcgc tgatgctgct ggcagctccc ttaatggcgc tggcaatctc accggccggt
     3061 ttcgccaaaa aacaattgac ggctatgtcc cgactgacta accgcttgcc tttcgcagcc
     3121 tggctcacgg gtgtcgccgt catgtggata tggcatgtcc cgggctggta taactctatc
     3181 actccggggg cggacactat ctcttgtgct ccgcttgctc ccgcattatt ctctcacctg
     3241 tcttcgatcg cgccattttc gcgacctttg atcatcgcag acagggtctt tccactgctg
     3301 aatgacctga gcctgcttat agccggtttc atcttctggt ggcctgtgat cactccctgt
     3361 cattccttcc ggctgtcccc gttgagaggc gtcgcgtacc tggcttcggc atgtatctgc
     3421 tgttcgctgc tcggactcct gatcacgttt gcgcgacccg gcgtctaccg cgggataagc
     3481 ctcagcgatc agcagaccgg cgggatgatc atgtggatcc cctgctgctt aatatacctg
     3541 acggcatcga tgggtctgct gatcgagtgg ctgtcgaaga aagaagccga cacctccatt
     3601 tccattcaaa atcatcacgc atgacacctg acatgactcc cgaagaaggt ttgcccgcac
     3661 ccgaacacca ggatgccctc ccgtcctccg caacggccgg cgagctgccg ccacccggcg
     3721 acctgtacc
//
LOCUS       DASYSV010000114         9284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2328817, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000114 DASYSV010000000
VERSION     DASYSV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9284
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328817"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="VGQ51_03680"
     CDS             <1..124
                     /locus_tag="VGQ51_03680"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEV9035689.1"
                     /translation="SVDHGTAFEIAGKGIASPDAMILAIEAAVRLSRYKKSQTA"
     gene            121..1233
                     /locus_tag="VGQ51_03685"
     CDS             121..1233
                     /locus_tag="VGQ51_03685"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEV9035690.1"
                     /translation="MIAVIADDLTGAAELGGIGVRYGLRTEIRTSAGTHTDADLLVIA
                     GDSRSKNEREAVEEMTGITRGLRLLQPGWVYKKTDSVLRGHVVAELEAHMQALGRHLA
                     LLVPANPSLGRTIHDGHYFLNGLPVHLSSFATDPEFPVTSSDIQDVLHARERPVFIRK
                     AADVLPERGIIVGEVHDKSNLQLWARRLPQQALPAGGAEFFSTLLELRLGRKPSGSSQ
                     PFVPGAPVLFVSGSTFDSSRKLIREQFDGGGPVSYMPRSQDTDWAAGVIRLLKDEGKA
                     IMAIDAAHRGHSALQLRQQMAEVVATVLKTSMPGELIIEGGSTAFAILEKQGWESLLP
                     EQELGPGTIRMRVPQTPGLFITVKPGSYRWPGSIRY"
     gene            1239..6500
                     /locus_tag="VGQ51_03690"
     CDS             1239..6500
                     /locus_tag="VGQ51_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEV9035691.1"
                     /translation="MNQSLHLPDILIIAIFLAATLFLGLRFARKKQSTQSYFLAKGKI
                     PAWAIGMSLLSSMISSVTFLAYPGEGYSSNWILLVQGLMVPVVLIGIIWFVVPLYRQV
                     IGLSTYEYFEKRFGVFARYYSSLAFVLRQFSAMGTIFFLLALALSKMTGMNTTAMIVA
                     IGCIIILINLLGGIEAVIWLDVLQGFLLFASGITCFLILLFGSAGSPSEAWHIAQAGH
                     HTGFGPYDWNFTRLTFVVMAINGVFYAIQKYGTDQTVVQRYLTARTDKAAIRASLMGV
                     LLTVPVWTLFMFIGTGLFVFYQSHPLPAGTRADAVFPYFIMTRLPVGLVGFILSAMTS
                     AAICSMSADLNSLGAVGVEDYYRKLRPGRPDGHYLRAGRWIVVAAGIVSIGIAMLYVN
                     AGSEGVLGIVFTLYAIFSGGISGIFLLGIFSPRTNRQGMNIAIVACILFTSYAFLTST
                     AIGVGEKKSVLMDLGSWNFTQNKLMLGVYSHLVVIVVGYFASLFFPRPDIDGRLLFSG
                     WRKARRLAPALGLLLVCGVGARAQESYKRSYEKGREALDHLASLYHNKTEWEARKSVL
                     RPCLIDALQLSHVPRSTAAVVSTPWRRMDGYEVSNIAIPIFPGLYVNGSLYRPLKRKG
                     RIPVILDPDGHWERQRYRADCQIRCAAEARMGAMAFNYDLFAWGESLLQFKTEDHRTP
                     MAETVQLLGGERILDYLLSLPDADTSRVGITGGSGGGTQDVLLTAIDSRIKVSVPVVM
                     VSAYFDGGCPCEHGRPVHECGGGTDNVEIAAMAAPRPQLLISDGGDWTAHMPEHDFPY
                     LQTIYGYYGATANVENVHLPNDHHDYGLSKRIPMYRFMASHLGLDIRAIEHRDGSIDE
                     SRCTVEPDSALYVFGPHGEHLPADAIMGFDNLQRAWEAHRGLKTVAAGQRYKVGVVDL
                     MLLKRQKLGAFGTARIVGADGLEVDMGGLGQRETFENTLADDSVRRVFLATAQADSLD
                     ICSLAMTGFYSQNFATRPTWQRMIGDCIATMRKMHVHIGFLPLGVNADPGRHPELRDS
                     VIRRLRIAGRMAVDAGVVIGIETTLPAAEELRLLREIGSPGIRSYFNFANACDAHRDI
                     SSELRTLGKEYICMIHCTNTDGVWLQNDSAIDMKKVKATLDSMGWSGWLVVERSRDAK
                     DVHNVKKNFGANVRYVRSVFQPAAEIWVAPNGSDAAAGKADAPLATLSAALRRARELR
                     RLDDPSVANGVHIRMKGGEYPLYEPVFLRPEDSGTGDGPTIIEAVAGETPVLSGGVPV
                     QGWRHVAGDRWETDIPAGFLFRELWVNGVKAIRARTPNDDDHMDRILSIDRRGRTIDI
                     PATQPLPDRPGNMEMVIHQMWAIAILRVKSIQRIGDRYRLSFKEPESHIEFEHPWPAP
                     VLDSAHKLNGSSAYYLTNAPEFLDQPGEWYEDIAQGKLYYRPRPGEDMNTAVVRAPAL
                     TQIVVIQGTQDRPVTNIRWKGITFSYAGWLRPSLEGHVPLQAGQYLLDAYKLKIPGTP
                     DKQSLENQAWIGRPPAAVEVAYGSGIVFRGCTFKHLASTGLDFVRGTHADTITRSLFR
                     DIGGTGLQIGVYSDPSFETHLPYDPADSREVCQDEWVTDNLVTDCTNEDWGCVGIGAG
                     YVHDVHIEHNEVSELSYSGICVGWGWTRTVNCMRNNRIIGNYVHHYAKHMFDVGGIYT
                     LSAQPGTLITGNRIDSIYHPPYAHDPNHWFYFYFDEGSSGITIRDNWCPGEKFMKNSN
                     GPGNEWENNGPMVADSIKSSAGIEKIK"
     gene            6502..7563
                     /locus_tag="VGQ51_03695"
     CDS             6502..7563
                     /locus_tag="VGQ51_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9035692.1"
                     /translation="MLRLGIVGLGEGRSTMSAALNSPKWQLKKICDLNEDLCRHRAEE
                     FGFPHYTLRYQDILDDPGIDVIGIYTPDKFHASHVMAALSAGKHVVCTKPFIDDLADA
                     RKIMDLVGSTGKKVFVGQSSRFFEPMRRQRKDYEDGLIGELITVEAYYHADHRWFLEK
                     GWSLEQAFKWLYGGLSHPVDLVRWYLPDIAEVMGYGMLSSNGRKGGLHHEDTMHFIFR
                     AADGRVARVSGAYTGPVQPVVRDSEMSCILRATAGCSQADYMDLRYAITDRAGEERVI
                     TWEHRLKHYFRFEGKSHHAGEYQNYLEYFHDCIETGVTAFPDVREGIGTIALLKAMEG
                     SLATGQPVRVESVLKRYGL"
     gene            7560..>9284
                     /locus_tag="VGQ51_03700"
     CDS             7560..>9284
                     /locus_tag="VGQ51_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV9035693.1"
                     /translation="MKDLLQPVDFIIVAVYLLALLGIGYWVSFVRRKKENENLFLAEH
                     SLGWASIGLNMWGTNVGPSMLLASASIGYTSGIVAGNFAWYAFVFILLLAVVFAPRYL
                     GAKVSTLPEFMGRRFGESTRNILAWYTLITILISWLSLGLFAGGILVRQLLGLPMWLS
                     VISMVVLATFFTAAGGLKAIAYTNVFQMLLLIAVSALLVFLGIRKIGGIGELFARTPH
                     SYWNLFLPAGDKNYPWPAILLGYPVMGVWFWCTEQSMVQSVLGARNLEQGQLGANFIG
                     WLKILDVPLFIIPGVLCFILYPHLSNPDEAYLTLVTRLFPTGLRGLIIVVLIAALVGN
                     IGSSLNSVSTVFTMDIYIKKYRPGATTREIIRIGRIMTLAGAAVSVLMALAIDNIKGL
                     NLFDVFQSVLGFLAPPMSVAFLLGILWKGTTARAINGVLTVGTLFSVGVGVLFLWVFP
                     ASRYPVWPHFLLLSFYIFVVITIGAVLFSLADRTASPAAPVIPVARPTRRVRLLWIAL
                     TAVMIVLYILFNGFSAAAQDAPPPSAASWIGYPGDYAIWLGNQVQNRRTERGTFFPPF
                     WKLDNHYVL"
BASE COUNT         1876 a         2579 c         2732 g         2097 t
ORIGIN      
        1 gagcgtcgat catggcaccg cgttcgagat cgccggtaag ggaattgcca gcccggatgc
       61 aatgatattg gccatcgaag cggcggtcag gctcagccgc tacaaaaaaa gtcagacagc
      121 atgatagccg tgatcgcgga cgatctgacg ggcgccgccg aattgggcgg catcggtgtc
      181 cgctatggac tcaggacgga gatccgcacc agcgcgggta cacatacgga cgccgatctg
      241 ttggtcatcg caggcgacag tcgttcgaag aacgagcgcg aggcggtgga agaaatgacc
      301 ggcatcaccc gcgggctccg gttgcttcaa cccgggtggg tctataaaaa gaccgattcc
      361 gtgttgcgcg gacatgtcgt cgcggaactg gaagcgcata tgcaggcgct gggtcgccac
      421 ctcgcgctgc tggtacccgc caacccgtcc ctgggccgca ccatccatga cggccattat
      481 tttctcaacg ggctacctgt ccatctaagc tcgtttgcta ccgacccgga atttccggtg
      541 accagcagcg atatacagga cgtgctgcat gcccgcgaac gacccgtgtt catccggaaa
      601 gctgccgacg tgctgcccga aagaggcatc atcgtaggcg aggtgcatga caaaagcaat
      661 ttgcagctat gggcgcggcg gcttccgcag caagcgctgc cggcaggcgg agcggagttc
      721 ttttctacac tgctggaact gcggctcggc aggaagccat ccgggtcatc gcaacccttc
      781 gtacccggcg cccccgttct cttcgtcagc ggcagcacct tcgacagcag ccggaagctc
      841 atacgcgagc aattcgacgg tggcggcccc gtcagctata tgccccgcag ccaggacacg
      901 gattgggcgg ccggcgtgat ccgtttattg aaggacgaag gcaaggccat catggccatc
      961 gacgcggcgc atcgcggaca ttccgcactt cagctgcgtc aacaaatggc ggaggtggta
     1021 gccaccgtcc taaaaacatc gatgcccggt gagctgatca tcgaaggcgg ctccacggcc
     1081 tttgccattt tggaaaagca aggctgggag agcctgctgc cggagcagga actgggtccc
     1141 ggcacgatcc ggatgcgggt accgcagact ccgggcctat tcatcaccgt gaaaccgggc
     1201 agctatcgct ggccgggatc catacgctat taaccactat gaaccagagc ttgcatcttc
     1261 ccgatatcct catcattgcc atattcctgg ccgcgaccct gttcctgggt ctgcgctttg
     1321 cccggaaaaa acaatccacg caatcctatt tccttgccaa aggaaagata cccgcatggg
     1381 ccatcggcat gtcgctgctt tcgagcatga tcagcagcgt gacctttctc gcctatcccg
     1441 gcgaaggtta ctcgtccaat tggatattgc tggtgcaggg attgatggtt ccggtcgtgc
     1501 tgataggcat catctggttt gtcgttccgc tgtatcgcca ggtcatcggt ctgagcacct
     1561 acgaatattt cgaaaaaaga ttcggggtct tcgcacggta ttatagctcg ctcgccttcg
     1621 tcctccgcca gttttcggcc atgggaacga tctttttcct gctggcgctg gcgttaagca
     1681 agatgaccgg gatgaacacc acggccatga tcgtggccat cggctgcatc atcatcctca
     1741 tcaatctcct gggcggtatc gaagcagtga tctggctgga cgtattgcag ggctttttgc
     1801 ttttcgcaag cggcatcacg tgttttttga tcttattgtt cggctcggcc gggtcaccct
     1861 cggaggcatg gcatatcgca caggcaggcc atcacaccgg attcggtcct tatgactgga
     1921 atttcacccg gctgaccttt gtcgtcatgg ccatcaacgg cgtcttctat gccattcaga
     1981 aatatggtac cgaccagacc gtagttcagc gttatctgac ggcccggaca gacaaggcgg
     2041 ccatacgtgc ctcattgatg ggcgtgctgc tgacagtgcc tgtctggaca ttgttcatgt
     2101 tcatcggcac gggtcttttt gtcttttacc agtcgcaccc gctgcctgcc ggaacccgtg
     2161 ccgatgccgt ctttccatat tttatcatga cgcgtcttcc cgttgggctg gtcggattca
     2221 tcctttcggc gatgacctcc gcggctatct gcagcatgag cgccgatctg aattcgctgg
     2281 gcgcggtggg cgtggaagat tattaccgca aacttcggcc gggccgtccc gatggccact
     2341 atctacgggc gggcagatgg atcgtcgttg cggcgggcat cgtatccatc ggcatcgcca
     2401 tgctgtatgt caatgcgggc agcgaaggtg tgctcgggat cgtctttacg ctgtatgcta
     2461 ttttttccgg cggcatctcc ggtatcttcc tgctgggcat ctttagcccc cgtacgaaca
     2521 gacagggaat gaacatcgcc atcgtcgcct gtatcctgtt tacctcctat gccttcctga
     2581 cctccaccgc catcggcgtg ggggaaaaga aatcggtgtt gatggatctg gggtcctgga
     2641 attttaccca gaataaattg atgctggggg tctacagcca cctggtggtt atcgtcgtgg
     2701 gctatttcgc cagcctgttc ttcccccggc ccgacatcga cggaagactg ctattcagcg
     2761 gctggcgcaa ggcccgaagg ctggcgcccg ctctgggact attgttggtc tgcggcgtgg
     2821 gtgcccgcgc gcaggaaagt tataaaagat cgtacgaaaa gggcagggaa gcgctcgatc
     2881 acctggcttc gctgtatcat aacaaaacag aatgggaagc aaggaaatcc gtgttgcgcc
     2941 cctgtttgat cgacgctttg caactctcgc atgttccgag atcgaccgcc gcggttgtga
     3001 gcacaccctg gcgtcggatg gatggatatg aagtgtcgaa tatcgccatt ccaatatttc
     3061 ccggtctgta tgtcaacgga tcgctatatc gcccgttgaa gagaaagggc cggatacccg
     3121 tcatcctgga tccagacgga cactgggagc gccagcgcta ccgtgcggat tgccagatcc
     3181 gttgtgcggc cgaagcccgc atgggcgcca tggcattcaa ctatgacctg tttgcctggg
     3241 gagagtcgtt attacaattc aaaaccgaag accaccgcac tccgatggcc gagacagttc
     3301 agctgctggg cggcgagcgc atcctcgatt atttgttgtc gctgcccgac gcggacactt
     3361 cgcgggtggg catcaccggc gggtccgggg gcggcacgca ggatgtgttg ctgacggcta
     3421 tagatagccg tataaaagtg agcgtgccgg tcgttatggt gtcggcatat ttcgatggag
     3481 gctgtccctg cgagcacgga cgtcccgtcc atgaatgcgg tggtgggacc gataacgtcg
     3541 agatcgccgc catggccgct ccgcgtccgc agctgctgat ctccgatggg ggcgactgga
     3601 cggcgcatat gcccgaacac gattttccct atctgcagac gatctatggc tattatggcg
     3661 ctacggccaa tgtggagaac gtccatctgc ccaacgacca tcatgattat ggtctttcca
     3721 aacggatacc gatgtatcga ttcatggcca gtcatctcgg cctggacatc cgggcgatag
     3781 aacacaggga cggcagtatc gacgaatccc ggtgtaccgt ggagcccgac tcggcgctat
     3841 atgtctttgg accgcatggc gagcacctgc ccgccgatgc catcatgggt ttcgataacc
     3901 tgcaacgggc atgggaagcc catcggggtt tgaagactgt tgcggccgga cagcgatata
     3961 aggtaggcgt cgtcgatcta atgttactca aacgccagaa gctgggcgca ttcggcacgg
     4021 ccaggatcgt cggcgcggat gggctggaag tggacatggg cgggttgggt cagcgcgaaa
     4081 catttgagaa tacgcttgcc gacgactcgg tacgaagggt ttttctcgct acggcacaag
     4141 ccgatagcct ggatatctgt tcgctggcga tgaccggttt ttactcgcag aatttcgcca
     4201 ctcgtcccac ctggcagcgt atgatcggcg attgtattgc taccatgcgc aagatgcacg
     4261 tgcatatcgg ttttctgccg ctgggcgtta atgccgaccc cgggagacat cctgaacttc
     4321 gcgacagtgt tatccggcga ttgcgcatcg cgggtcggat ggcggtcgat gcgggcgtcg
     4381 ttatcggtat cgagacgacg ttgccggcgg cggaggaatt gcggctgctg cgggagatcg
     4441 gatcgcccgg catcaggagc tatttcaatt ttgcgaatgc atgcgacgct catcgcgaca
     4501 tctccagcga actgcggact ctgggaaaag agtacatctg catgatccac tgcacgaata
     4561 ccgacggtgt ctggttgcag aacgatagcg cgatcgatat gaagaaggtg aaggccacgc
     4621 tggattcgat gggctggagc ggctggctgg tcgtcgaacg ctcgcgcgac gccaaagacg
     4681 tgcataatgt caaaaagaat tttggcgcca atgtccggta tgtcagatcc gtgtttcagc
     4741 ccgccgcgga gatatgggtc gcacccaatg ggagcgatgc ggctgcaggt aaggcggatg
     4801 ctccgcttgc gacattgtcc gcggcactgc gcagggcgcg tgagctgaga cgtctggatg
     4861 acccatcggt tgccaacggg gttcacatcc ggatgaaagg cggggaatat ccgttatatg
     4921 agccggtgtt tcttcgtccg gaagattccg gcaccggcga cggacctacg atcatcgaag
     4981 cggttgcggg cgagacaccc gtgcttagcg gaggagttcc ggtacagggt tggcggcatg
     5041 tggcgggcga tcgttgggag accgatattc ctgccgggtt tctcttccgc gaactgtggg
     5101 tcaatggggt caaggcgatc cgcgcgcgca caccaaatga cgatgatcat atggaccgta
     5161 tcctgtctat tgaccggcga gggcggacca tcgatatccc ggcgacgcaa ccactgccgg
     5221 atcggcccgg caacatggaa atggtgatcc accagatgtg ggctatcgcc atattgcgtg
     5281 tcaaaagcat tcaacgtatc ggagatcgtt accggctgag cttcaaagaa cccgagagcc
     5341 atatcgaatt cgaacatccg tggcccgccc ccgtgctcga cagcgcgcat aagctcaacg
     5401 gcagctcggc ctactatctg accaacgcgc cggaattcct cgatcagcca ggtgaatggt
     5461 atgaagacat cgcccagggg aagctctact accggccccg acccggtgaa gacatgaaca
     5521 ccgccgtggt ccgcgctccg gcgcttactc agatcgtcgt gatacagggc actcaagatc
     5581 gaccggtaac gaatatccgc tggaaaggga tcacgttctc ctatgccggc tggctccggc
     5641 cgtcgctgga aggtcatgtg ccgctgcagg caggtcaata ccttctggat gcctacaaat
     5701 tgaagatccc cggcacgccg gataaacaga gtctcgaaaa tcaggcctgg atcggtcggc
     5761 cgccggcagc ggtggaagtc gcctatggct ccggcatcgt cttccgcggc tgtacgttca
     5821 aacatctggc atcgaccggg cttgactttg tccggggcac ccatgcggat accattaccc
     5881 gcagtctttt ccgcgacatc ggcggcacgg gtttgcagat cggagtttac tccgacccgt
     5941 cattcgagac acatctgcca tatgatccgg ccgacagccg ggaagtatgc caggacgaat
     6001 gggtcaccga caacctggta acagactgca ccaatgaaga ctggggttgt gtcggcatcg
     6061 gcgccgggta cgttcacgac gtccatatcg aacataatga agtcagcgag ctctcctatt
     6121 cgggtatctg tgtcgggtgg gggtggacgc gaacggtcaa ctgcatgcgc aataaccgca
     6181 tcatcggcaa ctatgtccat cattacgcca aacatatgtt cgatgtgggc ggtatctata
     6241 ccctgtcggc ccaacccggc actctgatca ccggcaaccg catcgactcc atctatcatc
     6301 ccccgtatgc acatgatccg aatcactggt tctatttcta tttcgacgaa ggatcgtccg
     6361 gcattacgat cagggacaac tggtgtccgg gggaaaaatt catgaagaac tccaacggac
     6421 cgggtaatga atgggagaac aacggcccca tggtggccga cagtattaag agcagtgcag
     6481 gaattgaaaa aattaaatag tatgttacga ttaggaatag taggattggg tgagggccgc
     6541 agtaccatgt cggcagcgct caatagtccc aaatggcagc tgaaaaagat atgcgatctc
     6601 aatgaagacc tttgccgtca tcgcgcagaa gaattcggat ttccgcatta tacgctgcgc
     6661 taccaggata ttctcgatga ccccgggatc gatgtcatcg gcatctatac gcccgataaa
     6721 tttcatgcct cacatgtgat ggcggcattg tcggcgggta agcatgtcgt ctgcaccaaa
     6781 ccttttatcg acgatcttgc agacgcgcgg aagatcatgg acctcgtcgg gtccacgggg
     6841 aaaaaagttt tcgtggggca gagcagccgt tttttcgagc cgatgagacg gcagcgaaag
     6901 gattatgagg acggactgat cggcgagctg atcaccgtgg aagcctatta tcatgcggat
     6961 catcgctggt ttttggaaaa aggctggtcg ctcgagcaag ccttcaaatg gctgtatggc
     7021 ggccttagcc acccggtgga tcttgtccgg tggtacctgc ccgacatcgc cgaagtgatg
     7081 gggtatggga tgctcagctc caatgggaga aaaggcggac tgcatcatga agacacgatg
     7141 catttcatct tccgcgctgc agatggccgg gtggctcgcg tcagcggcgc gtataccgga
     7201 cccgttcaac ctgtcgtgcg cgacagcgag atgagctgta tcctccgggc cacggcgggc
     7261 tgcagccagg ccgactatat ggatctccgg tatgcgatca cggaccgcgc gggagaagag
     7321 cgggtcatca cctgggaaca ccggctgaag cattatttcc gttttgaagg caagagccat
     7381 catgccggag aatatcagaa ctatctcgag tatttccatg actgtatcga gactggcgtc
     7441 acggcctttc cggatgttcg ggaaggcatc ggcaccatcg ccctgctgaa agccatggaa
     7501 ggatcactgg cgacgggcca gcctgtaagg gtagagtcgg tgttaaaacg atatggacta
     7561 tgaaggacct gctgcaaccc gtggatttca tcatcgtggc cgtctacctg ctggcgctgc
     7621 tggggatcgg ttattgggta agttttgtgc ggcggaagaa agagaatgag aacctttttc
     7681 tcgcggagca ttcgctgggc tgggccagca tcgggttgaa catgtggggc accaatgtcg
     7741 gcccctccat gcttctcgca tcggccagca tcggttatac ctccggcatc gtggcgggca
     7801 atttcgcctg gtatgcattc gtattcatcc tgctgctggc agtggtcttc gccccgcgtt
     7861 atctgggcgc gaaggtctcc acgctgccgg aattcatggg acgccgtttc ggtgaatcga
     7921 cgcgcaacat cctggcctgg tatacgctga taacgatact catctcatgg ctttcgctgg
     7981 ggctttttgc cggcggcatt ctcgtccggc agctgctggg tcttcccatg tggctttccg
     8041 tgatcagtat ggtcgtgctg gccacctttt ttacggctgc cggcggactg aaggccatcg
     8101 catatactaa tgtattccag atgttgttgc tgatcgccgt atcggccctg ctcgtctttt
     8161 tgggtatccg aaagatcggc ggcatcggtg agctatttgc gaggacaccg cattcctatt
     8221 ggaatctgtt tcttccggca ggggataaga actatccctg gccggccatc ctgctgggtt
     8281 atcccgtgat gggcgtttgg ttctggtgta cggagcaatc gatggtccag tcggtgctgg
     8341 gtgcgcgcaa tctcgagcag ggacagctgg gcgccaattt catcggatgg ctgaagatac
     8401 tcgacgtgcc gctgttcatc attccgggtg tattgtgttt tatcctctat cctcatttgt
     8461 ccaaccccga tgaagcctat ctcacgctgg tgaccaggct tttccctacg ggtctgcgcg
     8521 gcctgatcat cgtggtgctc atcgccgcgc tggtaggcaa tatcggttct tccctcaatt
     8581 ccgtcagcac tgtttttacc atggatatct atattaaaaa atatcgtccc ggcgcgacta
     8641 cgcgcgagat cattcgcatc ggacggatca tgacgctggc aggggccgcc gtatccgtgt
     8701 tgatggcgct tgcgatcgac aatatcaagg ggttgaatct ttttgacgta ttccaaagcg
     8761 tccttggatt tttggcgccg ccgatgtcgg tggcttttct gctcggcatc ctttggaaag
     8821 ggacgacggc gcgcgcgatc aatggggtgc tgaccgtagg gaccttgttc agcgtcggcg
     8881 tcggcgtgct ttttttatgg gtattccccg cctcgcgtta cccggtatgg ccgcatttcc
     8941 tgttgctgtc gttttatatt ttcgtcgtca tcacgatcgg cgccgtattg ttttcgctgg
     9001 ctgatcgcac ggcgtctccc gcagccccgg tgatccccgt cgcgcgccct acgcgccgcg
     9061 tccgcctgct ctggatagct ctcaccgccg tgatgatcgt tttgtatatc ttattcaatg
     9121 gattcagcgc tgccgcgcag gatgccccgc caccatccgc cgcctcctgg atcggctatc
     9181 cgggagacta tgccatctgg ctaggcaacc aggtgcagaa ccgaaggacc gaacgcggaa
     9241 cctttttccc gccattctgg aaactggaca accattatgt cctg
//
LOCUS       DASYSV010000115         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1175556, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000115 DASYSV010000000
VERSION     DASYSV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175556"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VGQ51_03705"
     CDS             <1..587
                     /locus_tag="VGQ51_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9035694.1"
                     /translation="AKYGLISFASMACEGTMFDWSGIYFRKAVGAGREVATLGFVAYM
                     TAMTLGRLTGDRLANRFGIRTMLTYSGILIGSGLLIATLLPYPMTAGFGFILTGFGVS
                     CVIPMTFSMAGRSEGMSSGSAIAAVSTVGYLGFLAVPPLVGSVAQLAGLRSAWLMMAG
                     FGVLITVLVRLTLGNEKPMGSPDPAIMEADKMEC"
     gene            complement(636..1160)
                     /locus_tag="VGQ51_03710"
     CDS             complement(636..1160)
                     /locus_tag="VGQ51_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035695.1"
                     /translation="MSTPTCVRFEAGPDLSLFRFNTGFAGCAVFQLRFSALPKGIYDA
                     SIEYLHCFPGALYIPLTDVQIEELLLVMIQMIELYTLKYPERKIRLKADGQLQTMLFR
                     VMLLVHQDALLPWFTIVEERQRSLISFFRGRDSPTFFLKRRPDTSQPAPIQTILHTRS
                     RLFGNPMHVQVYEN"
     gene            complement(1211..2005)
                     /locus_tag="VGQ51_03715"
     CDS             complement(1211..2005)
                     /locus_tag="VGQ51_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035696.1"
                     /translation="MNKDNLAFLHDNLKYLGFGEYSLLNHLLESEMTNGTLSFELTTD
                     AFFDEDSRLEAKLYFRRSDQLDMYFFNKYDALLMYPDNPEKNKEQTFYIYKGSGVTLK
                     EAFNLLQGRAVYKKMTNSAGEKYTAWIQLNFEQKDLHENYKYKYFRSDNRYDLGKVLA
                     NYKLREMENEETREMLFQSLRRGNLHPVTLMKSNKTEKLLIHANPQFKTINIVPSATR
                     AVRKEKDNRKERCDVRETPDLAIPEREVNESVLEEDAEHAHQKNRQ"
BASE COUNT          462 a          509 c          585 g          567 t
ORIGIN      
        1 tggccaaata tgggttgatc tcgtttgctt ccatggcctg cgagggaacg atgttcgact
       61 ggagcggcat ctattttcga aaggccgtcg gcgccggcag ggaggtggcg acgcttggct
      121 tcgtagccta tatgacggcg atgacactgg gtagattgac gggggacagg ctggccaacc
      181 ggtttggtat acgcacgatg ctgacctata gcgggattct gattggatcg gggcttttga
      241 tcgccacctt gttgccttac ccgatgaccg ccggtttcgg ttttattctg acagggtttg
      301 gcgtttcctg cgtcatcccg atgaccttta gcatggcggg gcgatcggaa ggtatgagca
      361 gcggatcggc gattgcagcc gtgtcgacgg taggatacct cggattttta gcggtcccgc
      421 cattggtggg gtctgtggcg caattggcag gattaaggag cgcctggctg atgatggcgg
      481 gttttggagt gctgatcacg gtgctggtgc ggctgacact tggcaatgaa aagccgatgg
      541 gtagcccgga tcccgcgatc atggaggctg ataaaatgga atgctgagaa aaggcaaaag
      601 accgtccatc atttttagcg acggacggcc tattgttagt tttcataaac ctgcacatgc
      661 atcgggttgc cgaacaggcg gctgcgtgta tgcaatatgg tttgaatagg agcaggttga
      721 ctggtatccg gtcttcgctt caggaagaag gtgggactgt ccctgcccct gaaaaaggat
      781 attaacgatc tttgtcgctc ttcaacgatg gtaaaccatg ggagcagagc atcctgatgc
      841 acgagcagca tgaccctgaa gagcatcgtc tgcagctgcc cgtcggcctt caaccggatc
      901 tttctttccg ggtattttag cgtatataac tcgatcatct ggatcatcac cagcaacagc
      961 tcctctatct ggacatcggt caatgggata tagagggcgc cgggaaagca atgtaggtac
     1021 tctatcgacg cgtcatagat gcccttgggc agagcggaga agcgcagctg gaaaacggca
     1081 catcccgcga aaccggtatt gaaccggaac aggctcagat ccggaccggc ttcaaatcgg
     1141 acacatgtag gggtagacat tcgaaaaaag tttaaataga gcgaccgcta ccggtcgccc
     1201 cacaggatta ttattgacga ttcttttggt gcgcatgttc ggcatcttct tccagaaccg
     1261 actcgttcac ctctctttcg ggtatcgcca ggtcgggtgt ctccctgacg tcacaccgct
     1321 ctttacggtt atccttctct tttcggacgg ccctcgttgc cgacggaact atattgatcg
     1381 ttttgaattg aggattggca tgaatgagca gcttttccgt tttgttcgat ttcatcaggg
     1441 tcaccgggtg aagattgccg cgacggagcg attggaaaag catttctctc gtctcttcgt
     1501 tctccatttc ccgcagcttg taattggcca gcactttgcc cagatcatac cggttgtccg
     1561 atcggaaata cttgtattta tagttttcat ggaggtcctt ctgctcgaag ttcagctgga
     1621 tccaggcggt atatttctcg ccggccgagt tggtcatctt tttgtaaacg gccctgccct
     1681 gcaacaggtt gaaggcctct ttgagcgtaa cgcccgaacc cttatagata taaaaggtct
     1741 gctccttgtt cttttccggg ttgtccggat acattagcaa tgcgtcgtat ttattgaaga
     1801 aatacatgtc gagctgatcg gatcgccgga aatacaattt ggcctccagc ctgctgtctt
     1861 catcaaaaaa agcgtccgta gtgagctcga atgagagcgt tccattggtc atttccgact
     1921 cgagcagatg attcagcagt gaatattctc cgaatcccag gtatttcaga ttgtcgtgta
     1981 aaaaagcaag attatccttg ttcataacta tggttttttt gagtgaatag atcagatgag
     2041 tctggccctt gccagcatcc agttgcccac ggggatatgc agatgccggc ttccgttgct
     2101 ctcccgtacg tcgatccaaa gtc
//
LOCUS       DASYSV010000116        16530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_536443, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000116 DASYSV010000000
VERSION     DASYSV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 16530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16530
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536443"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VGQ51_03720"
     CDS             <1..1426
                     /locus_tag="VGQ51_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HEV9035697.1"
                     /translation="KVSVEGGTIDQLRTFYSCLYRMLFFPMRLYEKDATGRIVHYSPY
                     NGKIGTGYLFGGTGFWDTFRALYPFLNLLYPSINKEMQEGLVNDYKEGGWLPEWSAPG
                     YRNVMIGNNSASIVADAWVKGLRGYDIHTLYEALLHDANAAGPIDAVGRKGVDYYNTL
                     GYVPYDVGINENAARTLEYAYDDFSIYQLGKTLGRPADELKLYASRCQNYRHLFDPEH
                     KLMRGKNKDGSFESPFNPFKWGDAFTEGNSWHYTWGVFHDIQGLIDLIGGKSEFVRML
                     DSVFIMPPVFDDSYYGEVIHEIREMQIAGMGQYAHGNQPIQHMIYLYNYAGQPWKCQY
                     WVRQTMRRLYQGTPDGYCGDEDNGQTSAWYVFSALGFYSVCPATDQYVLGTPLFRKTV
                     LHLENGKEFVITAADNSETNLYVQNALLNGKVYTKNWIAHSVLEAGGQLQLTMGAVPN
                     KQKGINSDDFPYSFSTDKEAPKIK"
     gene            1423..3393
                     /locus_tag="VGQ51_03725"
     CDS             1423..3393
                     /locus_tag="VGQ51_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9035698.1"
                     /translation="MKQLFIFVCAALTASAGTAQDSATFFLHKFAQNIGKETYHRMTG
                     DSGVVYTVHFKFVDRGSAVPLEARMVETPAGDPVSLWIKGNTSRFSGIHDSVVLRDGK
                     AWITVDDSSYSQALEGPAFPVAGYSPGTVQMLLLQYWRQHGEPAVLHLLPTGAVSIRA
                     AGKDTLALYGQRLVLDRMVIHGLIWGNELVWTDEHGNLVCLITNDAEGDKLEMMRDRY
                     ETLLPEFIGRAATYGMALFAQQMHGDAAQPQTGTLAVVGGRMVDVATGETTNDAVILI
                     RKGKIVRTGARAAVAVPVGAKIIHAEGKTILPGLWDMHSHFEQAEWGPAYLAAGVTTV
                     RDCGNEFNYINAVKRAIDEGHGVGPHILKAGVIDGPGPMGLGIIRASNPYEARAAVRR
                     YKDSGFVQIKIYSSVKPPIVKAICDEAHRLGLTVTGHIPEGMTLIQGVDSGMDMVNHI
                     QYVGPILARTKDRLVDWSDPKTAAGFAFIKEHGTVIDPTLGVFEMVLRSVKDTITNME
                     PAFYSLPQPLQVLFRTMGMQPALAEKYKPMFGDMKKMVKTLYDEGVPIVAGTDMGFPG
                     WSVDRELELYVEAGLTPLQAIRTATIVPAMVMKQTAVSGTLMPGRNADLIIVDGDPLH
                     HIRDIRNVRVVVKDGKVYDPVELHRIAGFEVK"
     gene            complement(3569..4933)
                     /locus_tag="VGQ51_03730"
     CDS             complement(3569..4933)
                     /locus_tag="VGQ51_03730"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV9035699.1"
                     /translation="MLAIVKSAKSVRASLKYNEQKVEQRQARLLDAHNFWQEKEELTP
                     RDKWQRFNDLSTLNQRSQKHSVHITLNFPTKDDLSDRKMTEIATAFMERIGFAEQPWL
                     TYRHLDAGHPHMHIVSTNIRPDGSRIPNDLRSPAHLKQICFTMEEEYRLAPAIQMPDL
                     FEEKKAPSRKQNYGGEVGITYGEAGITYGDVGITSGEAGITYGEARVTYGKAPTKTEI
                     ARVLEHVVNQYAYSDLESYNAILSQYHVRADRGSENSPMYSNKGLYYRVVDEKGKKIG
                     APIKASAFDQPVKLQQLETMFRLNQAKLQQESQFLRNRIDSCVWFAGKPCSLGILKED
                     MMRENIDIVIDGRHGFFYIDHHAKIAIRDTDLGEQYSASAILRRTGIEQDIRQLANKN
                     RFRLTIGEQRVLSQPDGKNSSETLSLIFKLSGRHDEIVEHKLKLLEKEELSQHRGRGL
                     GRSR"
     gene            complement(4918..5238)
                     /locus_tag="VGQ51_03735"
     CDS             complement(4918..5238)
                     /locus_tag="VGQ51_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035700.1"
                     /translation="MKSEAKRFLSIRLSQDEFDKVQHYLQQSTCRSLTEYAKKTLTRQ
                     PVTVKVRDQTREDILHELGMIRSLLNKMPGSPDPSRVDPNVMSFVAEIYCCFREIAKK
                     CSQS"
     gene            complement(5348..6079)
                     /locus_tag="VGQ51_03740"
     CDS             complement(5348..6079)
                     /locus_tag="VGQ51_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035701.1"
                     /translation="MRIYFKYVLLAGLQWLAMRGLAQSASAKDDAFKFADSVFGRPAF
                     LFETLGPDIPADMQPVLIRMNNAISANKEWFIEYRNKYGGSGQALPYDEHFGISREDY
                     RKVQHMETQPPQLVVADSQKVAVSKDHGVIGFKSEGSTHLLDYLFIDIQHQRLMYGGD
                     TIPYNGRASADPSNPFGKWRGYSWRLERTDVNASLAAGKPTARVIEVDLGLPQIPGKT
                     YIRIEYQDIKAGVSTANMELIGYIR"
     gene            complement(6076..6720)
                     /locus_tag="VGQ51_03745"
     CDS             complement(6076..6720)
                     /locus_tag="VGQ51_03745"
                     /inference="COORDINATES: protein motif:HMM:NF025028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HEV9035702.1"
                     /translation="MLLLLLLPLLVASGYFYFGVEKRKTAVAAAKRDHDSVLVKLTDD
                     DFGCRIEKWTAGQFSRCFGEFYEDKRGEFFVKSFALVKGAHDSLREVDVFVRIPKLDS
                     GTFRSDGMYIMDNTKVICVFGTSDGGQYVVVKHADPKTFKAFRNVFGGKDSEHVFYEY
                     EIIPGIRPNKVKVLSATDNCANCDGYFRDGDLTYHNADKLEARFRIPQEYKYTE"
     gene            6846..6916
                     /locus_tag="VGQ51_03750"
     tRNA            6846..6916
                     /locus_tag="VGQ51_03750"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6878..6880,aa:Gln,seq:ctg)
     gene            7093..8313
                     /locus_tag="VGQ51_03755"
     CDS             7093..8313
                     /locus_tag="VGQ51_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEV9035703.1"
                     /translation="MVPSTAAPFALQRPLIAATPKNRIPSIDLLRGIVMLIMPIDHLR
                     DLLHFGHPLATDLHTTTAILFFTRWITHLCAPAFVFLSGVSAYLAGKRRTRQELAGFL
                     IKRGIWLLFIEVFVINLAVEVDPLYHLIVLQVIWAIGGSMILLGVLVWAKASPRVIGA
                     IGLVIFLGHNIIDVLHNDTIDNNPIWRLFVSAAGFSRHDPIGHGRYFVLAYALLPWTG
                     VMLLGYAIGTLYSQDAAKRRRTLTTIGLALLAFFVVFRAFNIYGDPAPWSAQKNSLFS
                     VMSFLNVTKYPCSLLYLCVTLGISLLILANTEGATNRLSRIVIVYGSVPFFYYIVHWF
                     LAQSITIILFFSLGHHMSEAYHSDFAFSTNTFGLPLAGVYGIWLLLVTVLYFPCRWFA
                     NYKKTHRQWWLSYL"
     gene            8919..9161
                     /locus_tag="VGQ51_03760"
     CDS             8919..9161
                     /locus_tag="VGQ51_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035704.1"
                     /translation="MPDLAIIPMRIEALRKLSEKKDVYLDDVQEIFRRDLQHFIMGET
                     ITQRNGKLVIGKRLYSQWLNKIKTKGFDYEIDFKND"
     gene            9154..9942
                     /locus_tag="VGQ51_03765"
     CDS             9154..9942
                     /locus_tag="VGQ51_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEV9035705.1"
                     /translation="MTEKLGIINRLKRMADEHAPQKDWDQAFLEKIKIDFTYNSNKIE
                     GNTITYGQTVKLLRDLVTPKNASTGEVLDMVNHQLVLTTVFKTYHSRDITEESIKELH
                     RLLMKNREQWSDDGLFNPGRYKDFENMTLRSTGKLHKYLPPTEVPLAMADLVAETNRR
                     IKSADINETEKHPLAIATFFHQEFLNKIHPFSDGNGRIGRIFTNLILIKEGYPPIFIQ
                     DVDRDTYLNRFELEETMPGAMLDFFVDKMIESLKIKIEFANLHR"
     gene            10214..10456
                     /locus_tag="VGQ51_03770"
     CDS             10214..10456
                     /locus_tag="VGQ51_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035706.1"
                     /translation="MSVPVSNIPFNQPSQPSTFAQMVDQLRTKSEAELKMLYLQFFKN
                     DLNEEWKSITETADFKNVSEEDIVIAIQKNRYTDHV"
     gene            complement(10792..13164)
                     /locus_tag="VGQ51_03775"
     CDS             complement(10792..13164)
                     /locus_tag="VGQ51_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035707.1"
                     /translation="MFKNYFKTAWRNLLKNKFYSLINIVGLTVGLAVCMLMMEYLEHE
                     RSYDSFHANADRICWVQSKVLLNNDSFFTPAVKYQTGPIVKSRVPGVDAFVRLKKERE
                     ATIVQNAQYPDLRFAEQYVAFADSNFFRFFSFKLLSGNKDEVLRNPFTVAISQRIARK
                     YFGTENPVGKVLRFNNTDNFVVSGVAEDAPSNSSITYDFLASMSSLAAYNKGEELKEN
                     EFATYFLLQKPSDGSRVETALLGLFREEKSPVKVDIRYIATMLPQTHLATGSANPSNV
                     KYLSVFPFVAALILLLAIINYSSLSTAGATARGKEIGVRKMIGAGRKAIVLQFFTESA
                     MFTSVAFILGYVLCIVGQAVFFRFLNIEIDNRFLYGPYVLLTFLGLFLVSVLLAGAYP
                     ALVLSSYKPLQVLYSRVIGIGGGVNLRRFFTVFQFTIAVTIMICGFVIRQQMDFFRHS
                     DTSLNRSNIVMIPFSKKAGRQYSALKNDIRSLPAVDMVSTSSYPMYKDYNISVIPGPN
                     GQGFIPLPSLQVDANFIPMLGIRWKIAPLDSTFSLHPGYSVINEAAVEKIGITGNPVN
                     QVVNGQFKVAGVVKDFDYSSLQNKIDAMVLSFNQENDTASGWAKNGGCLFARIKAGSN
                     VPTVIDGIKSVYSKYDHSAPFEYYFMDDAFNSMYSAEDKLHRIFMVFTLFTVVIACLG
                     LLGMSVFEVARRTKEISIRKVLGASIADIATRLSSDLLKPVLIAMAIASPIGWFCMHR
                     WLQGFAYRIHIQWWLLLMVGLLTGLIALITIGLQVVKAGLANPVASLKSE"
     gene            13461..15929
                     /locus_tag="VGQ51_03780"
     CDS             13461..15929
                     /locus_tag="VGQ51_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035708.1"
                     /translation="MFRSYLKIAIRSLGKSKGFTALNIIGLAAGLGVCLLIVLYVTDE
                     LSYDRYNTGADRIYRIDEDSYINNTRYTAATTSKFFGPALVASYPKIRQMVRFRNPGD
                     LLVRKGNGHVLDHRFVFADSTIFKVFTLPMIAGDPNTALNNPHSIVIDESAARRYFNS
                     TDVIGRTLEVGSDNTLLKITGVIRDMPEQSQFHFSFIRPLREANTFNDPDDNDWLSSN
                     YYTYILVQPGTTRAEVQKDVDAVASLHIGQALQEMVHSSEADLEKAGNHVGCQVFPLT
                     DVHLHSNKSSELEANSNIQFVYIFSVIAALILLIACVNFMNLSTARSANRAREVGIRK
                     VSGSTRGHLIIQFLTESILLSLFSLVLALAIAVLLLPMFNQLAGKSLNPDVLFSGRFL
                     PILIGLVLLVGCLAGSYPAFYLSSFQPIHVLKGKMAAGFKSSWLRNSLVVFQFFISIG
                     LIVCTLVIYRQLHYIRNKEVGFNRDQVLVIHGTWSLGLDGTANLRQNLLTLAGVTDAT
                     VTGDLPTVGGSQYAEPGWFRDASFDVRKATFMTTLRIDDHYIPTLGIQMVKGRNFDLA
                     QFPTDSTAIILNEAAVAMFGARDPLNMLLYNRADELKPNQLDHLKPLAFHVVGVVKDF
                     NYNSMHDKIRPLVMMVNMFNLGSMAVRFHTSDVSGLVRQVESKFQAAKQGLPFSYTFM
                     DNDFDKLYHAEQQTGRIFITFAVFAILIACLGLFGLVTYAAEQRTKEIGVRKVLGASV
                     SGIVGLLSRDFTRLVGIAALIAFPVAWWAMYKWLETFAYRTEISWWIFVVAGAVALAI
                     ALLTVSIQTIRAALANPVKSLRSE"
     gene            complement(15948..16304)
                     /locus_tag="VGQ51_03785"
     CDS             complement(15948..16304)
                     /locus_tag="VGQ51_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035709.1"
                     /translation="MADTVGQWQNTPDDIRFIHDILERYKQVRDDVTASDPIREGEPG
                     NSSETIEKINVRDGRGEVVLFSNGGHRRYITEHRVDPHIWCTSGASVHLDASGRAVID
                     VPFDRPGATIVFFGVL"
BASE COUNT         3858 a         4385 c         4218 g         4069 t
ORIGIN      
        1 aaaggtatcg gtcgaaggcg ggaccatcga tcagctcagg accttttatt cctgtctgta
       61 ccggatgttg ttcttcccga tgcgtctata cgagaaggac gccacgggcc gcatcgtgca
      121 ttatagtccc tacaacggaa agatcgggac cggctatctt tttgggggca ccggtttctg
      181 ggatacgttc cgcgcgttgt atcctttcct gaatctacta tatccgtcga taaataaaga
      241 gatgcaggaa gggctcgtaa acgattataa ggaaggggga tggctgccag aatggtcggc
      301 cccgggttat cgcaatgtga tgatcggcaa caactccgca tccatcgtgg cggatgcctg
      361 ggtaaagggg ctccgcgggt acgatatcca tacgctgtat gaggcattgc tgcatgacgc
      421 caatgcggcg ggcccgatcg atgccgtggg ccgtaaaggc gtcgattatt ataataccct
      481 gggttatgtt ccctatgacg tgggcatcaa cgagaatgcg gcgcggacgc tggaatatgc
      541 ctatgatgat ttcagcatct accagctggg taagacgctt gggcggccgg cggatgagtt
      601 gaagctctat gcgagccggt gtcagaacta ccgtcatctt ttcgatccgg agcacaagct
      661 catgagagga aagaacaaag acggcagctt cgagagcccg ttcaatccct tcaaatgggg
      721 ggacgccttc acggagggca acagctggca ctatacctgg ggcgtcttcc acgacatcca
      781 ggggcttatc gatctgatag gtgggaaaag cgaattcgtc cggatgctgg acagcgtgtt
      841 catcatgccg cccgtcttcg acgacagcta ttatggcgaa gtgatccatg agatccgcga
      901 gatgcagatc gcgggcatgg ggcaatatgc gcatggcaac cagccgatac agcacatgat
      961 ctatctttat aactatgccg gccagccgtg gaaatgccag tattgggtcc ggcagacgat
     1021 gcggcggttg taccagggca cgcccgacgg atattgcggt gatgaggaca acgggcagac
     1081 ctccgcgtgg tatgtcttct cggcattggg tttctattct gtctgtcccg ctacggacca
     1141 gtatgtcctg gggacgccgc tgttccggaa gacagttctg cacctggaga acgggaagga
     1201 gttcgtgatc acagcggctg acaatagcga gacgaacctg tacgtacaaa acgcgttatt
     1261 gaacggaaag gtctatacga agaactggat cgcgcattcg gtattggagg cgggaggtca
     1321 gcttcagctg acaatggggg ccgttccaaa taagcagaaa ggtataaatt cagacgattt
     1381 tccatacagt ttttcaacgg ataaggaggc accgaaaatc aaatgaaaca actattcatc
     1441 tttgtttgtg ccgcactgac ggcaagcgcg ggaaccgcgc aggacagcgc tactttcttc
     1501 ctgcataaat tcgcgcaaaa catcggcaaa gagacctatc accgaatgac gggtgacagc
     1561 ggtgtcgtct acacggtaca tttcaaattc gtggaccgcg gctcggcagt gccgctggaa
     1621 gcccggatgg tggagacgcc tgcgggtgat cccgtaagcc tttggataaa gggcaacacg
     1681 tctcgttttt cgggcatcca tgacagcgtc gtactgcgag acggaaaggc ctggatcacc
     1741 gtagacgaca gcagctatag ccaggcgctg gagggcccgg cgttcccggt ggcgggatat
     1801 tcaccgggga cggtacagat gttattattg caatactggc ggcagcacgg cgagccggcc
     1861 gtcctccatc tcttgcctac gggcgccgta tcgatcaggg cggcgggtaa ggatacgctg
     1921 gcgctttacg gacagcggct ggtgctggac aggatggtca tccatggtct gatctggggg
     1981 aatgagctgg tgtggacgga tgaacacggc aatctcgtct gcctgatcac caatgatgcg
     2041 gaaggtgaca agctggagat gatgcgcgac cgttatgaaa cgctgttgcc cgagttcatc
     2101 gggcgggcgg ccacgtatgg gatggcgctt tttgcgcagc agatgcatgg agacgcggcg
     2161 cagccgcaga cggggacgtt ggcggtcgtc ggaggccgga tggtcgacgt agccacaggc
     2221 gagacgacca atgatgcagt gatcctgatc cgaaagggga aaatagtcag gaccggcgct
     2281 cgcgccgctg ttgccgttcc cgttggcgcg aagatcattc atgccgaagg caagaccatc
     2341 cttcccggac tttgggatat gcactcgcat ttcgaacagg cggagtgggg gccggcctat
     2401 ctggccgcgg gcgtgacgac ggtgcgcgac tgcggaaatg agttcaacta tatcaatgcc
     2461 gtcaaacgtg ccatcgacga aggtcatggt gttggtccgc atattttgaa agccggcgtc
     2521 atcgacggcc ccgggccgat ggggctgggt atcatccgcg cctccaaccc ctatgaagcc
     2581 agggcggcgg ttcggcgtta taaagattcc ggttttgttc agataaagat ttatagcagc
     2641 gtcaagccgc cgatcgtaaa ggcgatctgc gatgaagcgc accggctggg cctgaccgtt
     2701 accggccata tccccgaagg gatgaccctg atacagggag tggattcggg aatggatatg
     2761 gtgaatcata ttcaatatgt cggccctatc ctcgcacgca cgaaggaccg gctcgtcgat
     2821 tggagcgatc cgaagacggc cgcgggtttt gccttcataa aagagcatgg gacggtcatc
     2881 gatccgactc tcggcgtatt cgaaatggtg ctccggtcgg tgaaggatac gatcacgaac
     2941 atggaaccgg cattctactc gctgccgcag cccttgcagg tattgttccg gaccatgggc
     3001 atgcaacccg cattggcgga gaaatacaag cccatgttcg gggatatgaa gaaaatggtc
     3061 aagaccttgt atgacgaggg cgtaccgatc gtggcgggca ccgacatggg cttccccggt
     3121 tggagtgtgg accgcgagct cgagctctat gtggaggcag gcctgacgcc cctgcaggcg
     3181 atccggaccg ccacgatcgt ccccgcgatg gtcatgaaac agacggcggt cagcggcacc
     3241 ctgatgcccg gccgcaatgc cgatctgatc atcgtcgacg gcgatcccct ccaccatatc
     3301 cgggatatcc gcaatgtgcg ggtggtcgtc aaagatggaa aggtctatga ccccgtggaa
     3361 ctgcatcgta tcgcggggtt cgaagtaaaa tagggcccgc gccacagcca cctcaccgcg
     3421 accggccgcg ccccccaccg cgcccccgcc atcaccgcga ccctcctcag cgcgcccccc
     3481 ctcagcgcgc cgcgcccctc agcgcgcccc caccgcgccc ccgccgcatc cccgccatca
     3541 ccgcgacccc ctcagcgcgc ctgccacatc accgcgaccg acccagtccc cgcccgcgat
     3601 gctggctgag ctcttctttt tccaatagct tcaatttatg ctccacgatc tcatcgtgac
     3661 ggccggacaa tttaaaaata aggcttagcg tttcggagga gtttttacca tcgggttggg
     3721 ataagacccg ctgttctcca atggtcagcc gaaaccggtt cttattggca agctgacgga
     3781 tatcctgctc gatgcccgtc ctgcgtagta tggcggaagc gctatactgc tcgccaagat
     3841 ccgtgtcgcg gatagcgatc ttagcgtgat ggtcgatata gaaaaagcca tgcctgccgt
     3901 cgataacgat atcgatattc tcccgcatca tgtcttcctt taaaatgcca aggctgcagg
     3961 gtttacctgc gaaccagacg caactatcta tacgattgcg cagaaactgc gactcctgct
     4021 gcagttttgc ctggttcagc cggaacatcg tttcgagttg ctgcagtttt accggttggt
     4081 caaaggcgct ggcttttatc ggtgcgccga tttttttccc cttctcgtcg acgacccggt
     4141 aatacagccc cttattgcta tacatcgggc tgttttcgct gccacggtcc gcccgcacgt
     4201 gatactggct caggatggca ttgtacgatt cgagatcgga gtaagcatat tggttgacaa
     4261 cgtgctccag cacacgggcg atctccgttt ttgtcggggc tttgccatac gtgacccggg
     4321 cctcgccgta cgtgatcccc gcctcaccgg aagtgatccc cacatcgccg tacgtgatcc
     4381 ccgcctcgcc gtacgtgatc cccacctcgc ctccgtagtt ttgctttctt gaaggcgcct
     4441 tcttttcctc gaaaagatcg ggcatttgga tggcgggggc gaggcggtat tcctcttcca
     4501 tcgtgaagca aatctgtttc agatgggcgg gtgaccgcag atcattcggg atgcgactgc
     4561 catcgggtcg gatgttcgtt gaaacgatgt gcatatgcgg gtgtccggca tcgagatgcc
     4621 ggtaggtcaa ccacggttgt tcggcaaacc cgatccgttc cataaacgcg gtcgcgatct
     4681 ctgtcatctt ccgatcggac agatcgtcct tcgtcggaaa attcagtgtg atgtggacgg
     4741 agtgtttttg ggaacgttga ttcaatgttg acaggtcgtt aaaccgctgc catttatcgc
     4801 gcggagtcaa ctcctctttt tcctgccaga aattgtgcgc gtccagcagg cgcgcctgtc
     4861 gttgttcgac tttttgttcg ttgtatttaa gcgaagcgcg aacgctcttc gccgatttta
     4921 cgattgcgag cattttttgg caatttcacg aaagcagcaa tagatctcgg cgacaaaact
     4981 catgacattg ggatcgaccc tcgaagggtc cggcgatccg ggcatcttgt tgagaagact
     5041 tctgatcatg cccagctcat gaaggatatc ctcacgggtt tggtcgcgaa ctttgacggt
     5101 aaccggctga cgggtcaacg ttttttttgc atattcggtc agactgcgac atgtggactg
     5161 ctggagataa tgctggacct tatcgaattc gtcctgggat agacgaatcg agagaaaacg
     5221 ctttgcttca cttttcatac atcctcaatt tcaggataaa gatagttgga taatccgaaa
     5281 atcgatcgat ctaagtcgga attcgattca cgggcgtgac acgcgcgcct cgtgtaaatg
     5341 ggtagtccta acggatatat ccgatgagct ccatgtttgc cgtagagacc cccgccttga
     5401 tatcctggta ttcgatccga atataggttt tgccgggtat ctgcggtagc cccagatcga
     5461 cctcgatgac gcgggctgtg ggtttgccgg ctgcgaggga agcgttgaca tcggtccttt
     5521 ccagccgcca ggaatagccc cgccattttc cgaagggatt ggagggatcg gcgctggctc
     5581 gcccgttata gggaatcgta tcgccgccat acatcagccg ttgatgttgt atgtcgatga
     5641 aaaggtagtc gagaaggtgg gtgcttcctt cgcttttgaa gccgatgacg ccgtggtctt
     5701 tgctgaccgc taccttttgc gaatccgcga ccaccaattg gggtggttgt gtctccatat
     5761 gctgcacctt gcgataatcc tcgcggctga tgccgaaatg ttcgtcatac ggcagggcct
     5821 gcccgctccc gccatattta ttccggtatt cgatgaacca ttccttattg gcgctgatgg
     5881 cgttgttcat ccggatgagg acgggctgca tgtcggccgg tatatccggc ccgagggtct
     5941 cgaagaggaa ggcgggacgc ccgaaaaccg agtcggcaaa tttgaatgcg tcgtccttcg
     6001 cgcttgcgct ttgggccaaa ccgcgcatcg ccaaccactg aaggcccgcc aataaaacgt
     6061 atttgaaata aatcctcatt cagtgtattt gtattcttgc gggatacgaa accgcgcctc
     6121 caatttatcc gcgttatgat aggtcagatc gccatccctg aaatagccgt cgcagttagc
     6181 acaattatcc gtcgcactta acaccttcac cttatttggc ctgatacccg gtattatttc
     6241 atattcataa aaaacatgct cgctatcctt tcccccgaac acatttctga acgccttgaa
     6301 tgttttcggg tcggcatgct ttaccaccac atactgcccc ccatcagaag taccgaaaac
     6361 gcagatcact tttgtgttat ccatgatata cataccgtcg ctgcggaaag tcccggaatc
     6421 caatttcggg atccggacaa atacatccac ttcccgaagt gaatcgtgtg cacctttcac
     6481 cagtgcaaaa cttttcacaa aaaactcccc ccttttatcc tcgtagaact cgccgaaaca
     6541 ccgcgaaaac tgccccgccg tccatttttc gatccggcag ccgaaatcat catccgtcaa
     6601 tttcaccagg acactgtcat ggtctctttt cgccgccgcg accgccgtct tccttttttc
     6661 caccccaaaa tagaaatagc ctgacgctac caacaaaggc aacagcaata gtagtagcat
     6721 aaccgatttt ttcatcggat gggttcatcc tcctttcgca aaaataacgc attgattgct
     6781 agcaccttga ttttaatcct tgaggttttc gccattccaa aaaaatcgta ccttcgcggt
     6841 cccattgccc aatagtataa tggtagtaca gaagattctg gatcttttag tcttggttcg
     6901 aatccaggtt gggcaacaac gagccccgaa gctttgcttc ggggctcgtt gggtaatgca
     6961 aatacaggtg gatcagcccg tcatcccccc ggatgacggg aataatccag tcaggcaaca
     7021 gctctacctc tcgtcgcgtt tgcggcgggc tttttttatg ctggtttgta aatagttgtc
     7081 tatctttcat acatggttcc atctacggcg gctccctttg ccctgcaaag acccctaatt
     7141 gccgcaaccc ccaaaaaccg cattccctcc atcgatctgc ttcgtgggat agtgatgctt
     7201 attatgccca tcgatcacct cagggacttg cttcatttcg gccacccctt ggcgaccgac
     7261 ctgcatacga ccacggcgat acttttcttt acgcgatgga tcacccatct ctgcgccccg
     7321 gcttttgtat tcctgagcgg cgtttctgct tatctcgccg gcaagcggcg gaccaggcaa
     7381 gaattggccg gctttctgat caagagaggt atctggctgc tttttatcga ggtcttcgtt
     7441 atcaacttgg ccgttgaggt agatccgctt taccacctga tcgtgctaca ggtgatctgg
     7501 gcaattggcg gcagcatgat cctcctcggc gttttggtat gggcaaaagc gagtccgagg
     7561 gtgatcgggg cgatcggatt ggtgatcttc cttggtcata atatcatcga cgtgctccac
     7621 aacgacacga tcgacaataa tccaatttgg cggctgtttg tttcggctgc cggattcagc
     7681 agacatgacc cgataggtca tggacggtat tttgttttgg cctatgccct cctgccatgg
     7741 acgggggtaa tgctcctggg ttatgccatc ggaacgctgt acagccagga tgccgcgaaa
     7801 cggagacgga ccttgacaac catcggtctg gcattgctgg cgttttttgt cgttttccgg
     7861 gcgttcaata tttatggcga tcccgcgccg tggtcggcgc aaaagaattc tctgttcagc
     7921 gtgatgtcgt ttctgaatgt caccaaatat ccgtgctcgc tactgtatct gtgcgtgaca
     7981 ctcgggatct cgttactgat cctggcgaat accgaaggcg ctaccaaccg gctttcgcgg
     8041 atcgtgatcg tatatggcag tgttcctttt ttctattata tcgttcactg gttcctcgcg
     8101 caatcgatca cgatcatcct tttcttttcg ttggggcatc acatgagcga agcctatcac
     8161 agcgactttg cttttagtac gaatactttt ggcctcccgc tagcgggggt atatgggatt
     8221 tggctgttgc tggttacggt gctgtatttc ccttgccggt ggtttgcgaa ttacaagaag
     8281 acgcacaggc agtggtggtt gagctatttg tgagtgtcag tcaccgtgat tccacttttg
     8341 ttaaacaaga taaaaggtgt attagcaatc agatcatgcg cttaattctg aaataagcct
     8401 caaatccgcc aggaattggt tcgagaaacg tccagtcggg gcaacaaaac taatatagaa
     8461 tctttttcta gcgtttttat gacagattta ctgattgtta taaagactct acgacgaagg
     8521 ttctattttt ctttcgggca atgaaccaat attttctgcg attttgctta attcccaacg
     8581 ttcttacttt caatagcctg catgactttt tgacaattat taaatctatt tacaagtcca
     8641 tttccagcct aatccggagg acgatcgaga tagacttttt tcagcctacc aattggatca
     8701 ttgttcggtg caagaagtgc ggtgagctga acttgctgga acccggcgtc ggaatcgccg
     8761 ccggtagccc ggtgggaaaa gagaccgagg tttgctttgg cgcaatggga tcattataag
     8821 acaaaaaatc gtcgacttaa acgcccttcc ttaatgataa tgaatggctt gcaatctcca
     8881 aacaatttga gtaattttga cttataaaga ttttgaccat gcccgatctg gcgataatac
     8941 caatgcgcat tgaggcgctg cgcaaacttt ctgaaaagaa agatgtttac ctggatgacg
     9001 tccaggaaat ctttcgaagg gatcttcaac actttattat gggagaaaca atcacccaac
     9061 gcaatggcaa attggttatt ggaaagaggc tctattcgca atggctgaat aagatcaaga
     9121 ccaaaggatt tgactacgaa atagacttta agaatgactg aaaagctggg catcatcaat
     9181 aggctgaagc gtatggcgga cgaacatgcg ccacagaaag attgggatca ggcattttta
     9241 gaaaaaatta agatcgattt tacctacaat tcgaataaaa tagagggcaa cacgatcacg
     9301 tatggccaaa ccgtcaagct gctacgcgat ttggtgaccc ccaaaaatgc ttcgaccggc
     9361 gaggttctag acatggttaa tcatcaactc gtcctgacga cggtattcaa gacttatcat
     9421 tccagggaca tcacggagga aagtatcaag gaactgcacc gactgttgat gaagaaccgc
     9481 gagcaatgga gcgatgacgg cctcttcaac cccgggcgat acaaggattt cgagaatatg
     9541 acgcttcgat ccaccggaaa gctgcataaa tatttgccac caacggaagt accattggca
     9601 atggccgacc tggtcgcgga gacgaatagg cgcattaaga gcgctgatat caatgagacc
     9661 gaaaaacatc ctttagcgat cgcgaccttt ttccatcagg aatttctcaa taaaatccat
     9721 ccgttcagcg atggaaatgg gcggataggc cggattttca ccaatctgat cttgatcaag
     9781 gaaggatatc ctcccatttt tatccaggat gtagatcggg atacctactt gaaccgtttt
     9841 gagttggaag aaacaatgcc tggtgccatg ctcgattttt tcgtcgacaa gatgatagaa
     9901 agcctgaaga tcaagatcga atttgctaat ttacaccgat aggaacattc gggccgcttg
     9961 atggccgata tctgaggtca ttgggtaaat tggcggctag tttcctgcgg gtaaatatct
    10021 cttaccatgt gtttccattt gcgtttccac tctcatagta tgaattgatt tacaatgctt
    10081 tttcataccc aggttgggca acaagtgacc cctcgctttg cgaggggttt gtctttcgac
    10141 ggcgtggcaa gtacctgaat atctactaat taactcgttt cgatctatcg aaaattaagt
    10201 ttaaatttgt gatatgtcag ttccggttag taatattcca ttcaatcaac catcccagcc
    10261 atcgactttt gcgcagatgg tggatcaact tcgtactaaa agcgaagctg agcttaaaat
    10321 gctctatttg caattcttca aaaatgattt gaatgaggaa tggaaaagca tcaccgaaac
    10381 tgcagacttc aagaacgtct ccgaagaaga cattgtaata gccattcaaa agaatcgtta
    10441 tacggatcat gtataatctt cttgttgatg ccaatgtttg gataaaattt gcaagaagca
    10501 agaacatctc cccattgtta gacagatttg tgttttcatc attaatgatg attcaaaact
    10561 gctgcgtttc aatctgaaac cagttccggt ccatggtacc agttggttcc tcaaaaaatt
    10621 tccgcttggc ggagaagcat aagcatagtg gtaggccttt acaatatctt tatatatgac
    10681 tttccaaaga tgagctggca cgattcacct atttacggaa tggcattctt cagtgattcc
    10741 ctaacagtgt ccaatgagct gcttcgggtt tttgttttta ggagcaaaag ttcattccga
    10801 cttcaggctc gcaaccgggt ttgccaaccc tgccttcacg acctgtaaac cgatcgttat
    10861 cagagcaatc aatcctgtta gtaggcctac catcagcaat aaccaccact gaatgtgaat
    10921 gcggtaggca aacccctgta gccaccggtg catgcaaaac cacccgatgg gcgatgcgat
    10981 ggccattgca atcagcacag gcttcagcag gtcagacgat aatctggtgg ctatgtctgc
    11041 tatgctggcg ccaagcacct tcctgatact gatctctttt gtacgccggg caacttcaaa
    11101 aacgctcata cccaggaggc caaggcatgc tatcaccaca gtgaacaagg taaagaccat
    11161 gaatatcctg tgcaatttat cttctgcgct gtacatacta ttgaatgcgt cgtccataaa
    11221 ataatactcg aaaggtgcgc tgtggtcata ttttgaatac actgacttga ttccatcgat
    11281 aacggtgggg acattactac cagcttttat ccgggcaaaa agacatccgc cgtttttggc
    11341 ccatccggac gcggtatcat tttcctggtt aaaggaaagc accatggcgt caatcttgtt
    11401 ttgaagcgag gagtaatcga agtcttttac cactccggcc actttaaact gcccatttac
    11461 cacctggttc accgggttgc cggtaatccc gatcttttcg acggctgcct cgtttatgac
    11521 ggaataaccg ggatgcaggc taaaagtaga atccagaggc gcaatcttcc agcgaatccc
    11581 tagcataggg atgaaattgg catccacttg caagcttggc agcggtatga atccctgtcc
    11641 gttcggcccc ggaatgacgc tgatattata gtccttatac atggggtagc tgctggtact
    11701 caccatgtca acagcaggca aagaccggat gtcatttttc aacgccgaat attgcctgcc
    11761 ggcctttttt gaaaacggta tcataacaat attgctgcgg ttcaggctgg tatcggagtg
    11821 tctgaaaaaa tccatttgct gacggatgac aaatccgcag atcatgatcg taacggcaat
    11881 agtaaattgg aaaacagtga agaatctacg taggttcacg ccgccgccaa ttccgattac
    11941 acgactatac aggacctgca agggtttata agaagatagc accagtgccg ggtaagctcc
    12001 agccagcaac acagacacca ggaacagacc caaaaatgtc aacaatacgt atggaccgta
    12061 caagaaccgg ttgtcgattt cgatattcaa gaagcgaaaa aagaccgctt gacccactat
    12121 gcaaaggaca tagcccagta tgaacgccac ggacgtaaac atggccgatt ctgtaaaaaa
    12181 ttgcaggact attgcttttc gccccgctcc gatcattttt cttacgccga tctcttttcc
    12241 cctggccgtc gcgccggccg tagaaaggct gctgtaattg atgatggcca gcaataagat
    12301 caatgccgcc acaaaaggaa agacagaaag gtatttaaca ttggatggat tagcggaacc
    12361 agtggctaga tgcgtttgag ggagcatggt tgctatatag cggatatcca ctttgacggg
    12421 tgacttttcc tccctgaaga ggcccagcag cgccgtctcc acgcggctgc catccgatgg
    12481 tttctgtaac aggaaatacg tcgcaaactc attctccttt agctcttcac ctttattgta
    12541 tgcagccaaa gaagacatgg aagcgagaaa atcgtaggta atacttgaat tggaaggtgc
    12601 gtcctcagct accccactca cgacaaagtt gtccgtattg ttgaatcgca atacttttcc
    12661 gaccgggttt tcggtcccga aatatttcct ggctattctt tgggatatgg cgaccgtaaa
    12721 aggattccgt aacacctcgt ccttgttgcc ggatagcagc ttaaaggaaa aaaaacgaaa
    12781 gaaattgctg tcggcaaatg ccacatattg ttcagcgaat cgcagatcgg gatattgagc
    12841 attttgaaca atagttgctt ccctttcttt cttaagacga acaaaagcat ccactccagg
    12901 tacgcggctt ttcactatcg gaccagtctg gtatttcact gcgggcgtaa agaatgaatc
    12961 gttgttcagc aggacttttg actgcaccca acagatgcgg tcggcgttgg catgaaaact
    13021 atcgtaactg cgctcatgtt ccagatattc catcatcagc atgcaaactg ccagaccgac
    13081 ggtcagccca acaatattga tcagcgaata aaatttattc ttcagcaggt tgcgccaggc
    13141 agtcttgaaa tagtttttaa acatggcaga agattgggag tgcaataaat atatggtctt
    13201 ttctattcag taagaatgct gccgcgccgt gttataagag ctttaacaat gctgggccga
    13261 ctcatgaccg cggattttgc aaattccccg catacccccg ccgatgctcg ttcctgcacg
    13321 atcaatgtat cgaaaccgaa cattatggtc tctcgcaggt catgacagaa ggatggtctt
    13381 caagacttta taaacccggc attcttttgg gatcatcccc ctgatctggc cgccttactc
    13441 gtcataaaac cgaccatccc atgttccgca gctatctcaa gatcgccatc cgcagcctcg
    13501 ggaaaagcaa gggctttacc gccctcaaca tcatcggcct cgcggccggg ctcggtgtct
    13561 gtctgctgat cgtcctgtac gtcaccgatg agctcagtta cgaccgctac aacacgggcg
    13621 ccgaccgcat ctatcggatc gatgaggata gctacatcaa taacacccgg tacaccgctg
    13681 caaccacctc caagtttttc ggccccgctc tcgtcgcttc ctatccgaag atccggcaga
    13741 tggtcaggtt ccgcaatccg ggcgacctgc tggtgaggaa gggtaacggc cacgtcctcg
    13801 accatcgttt cgtcttcgcc gactcgacga tcttcaaggt cttcaccctg cccatgatcg
    13861 ccggcgaccc caataccgct ctcaacaacc ctcactccat tgtgatcgat gaaagcgccg
    13921 cgcgccgcta tttcaacagc accgatgtaa tcggccgcac gctggaggtg ggtagtgaca
    13981 atacactcct caagatcacc ggggtcatcc gggacatgcc ggaacagtcc cagttccatt
    14041 tcagcttcat ccgccccctc cgcgaagcca acacctttaa tgaccccgac gacaacgact
    14101 ggttaagcag taattattat acctatatcc tggtccaacc cggcaccacg cgagccgagg
    14161 tccagaagga tgtagacgca gtcgccagcc tccatatcgg acaggcccta caggaaatgg
    14221 tccacagttc ggaggccgac ctcgagaaag cgggtaatca tgtcgggtgc caggtcttcc
    14281 ccctcaccga cgtgcacctc cattccaata agtccagtga gctcgaagcc aacagcaata
    14341 tccagttcgt atacatcttt tccgtcatcg ctgcgctcat cctcctgatc gcctgcgtca
    14401 atttcatgaa cctgtccacc gcccgctcgg ccaaccgggc cagggaagtg ggcattcgta
    14461 aggtctccgg ctcgacaaga ggacacctca tcatccagtt cctcaccgag tccatcctcc
    14521 tcagcctctt ctccctcgtc ctcgccctgg ccatcgctgt cctgctgcta ccgatgttca
    14581 atcagcttgc cggtaaatcg ctcaaccccg acgtgctttt cagcggccgg ttcctgccca
    14641 tcctcatcgg cctggtgctg ttggtcggct gtctggcagg gagctacccc gccttctacc
    14701 tttcctcctt tcagcctatt catgtactca aaggaaagat ggcggcaggc ttcaagagca
    14761 gctggctccg gaacagcctg gtggtctttc aattttttat ttctattggc ctcatcgtct
    14821 gcaccctcgt catctatcgc cagttgcact atattcgaaa taaggaagtc ggctttaacc
    14881 gggatcaggt actcgtcatc catgggacct ggtcgctcgg cctggatggt acggccaatc
    14941 ttcgccagaa cctgctcact cttgccgggg tcaccgacgc caccgtcacc ggcgacctcc
    15001 ccaccgtcgg cggcagccag tacgcggagc caggttggtt ccgggatgcc tcgttcgacg
    15061 tcaggaaggc gacattcatg accacgctcc gtatcgacga ccattatatt cccaccctcg
    15121 gcatacagat ggtcaaaggc cgcaacttcg acctcgcgca atttcctacg gactccaccg
    15181 ccattatcct caatgaagcc gcggtagcga tgttcggcgc cagggacccg ctcaatatgc
    15241 tcctctacaa tcgggcggat gagctcaagc ccaatcaatt ggatcacttg aagcctctcg
    15301 cctttcatgt cgtcggggtt gtaaaggatt tcaactataa ctccatgcat gacaagatac
    15361 ggccccttgt catgatggtc aacatgttta acttgggcag catggcagtc cgcttccaca
    15421 ccagcgatgt ttccggtctc gtccggcagg tagaaagtaa gttccaagcc gctaagcaag
    15481 gactgccgtt cagctacacc ttcatggata acgactttga caaattgtat cacgccgagc
    15541 agcagaccgg ccggatcttc atcaccttcg ccgtcttcgc catccttatt gcctgtctcg
    15601 gtctctttgg cctcgttacc tacgctgccg aacaacgcac caaggagatc ggtgtccgca
    15661 aggtcctcgg cgccagcgtc agtggtatcg tcggcctact cagcagggac ttcacgaggc
    15721 tggtcgggat cgctgcgctc atcgcgtttc ctgtcgcgtg gtgggccatg tacaaatggc
    15781 tggagacgtt tgcctatcgc acggagatca gctggtggat attcgtcgtg gccggcgccg
    15841 tcgccctcgc cattgcgctg ttgacggtga gtattcagac catccgcgcg gccctcgcta
    15901 accccgtaaa atctctccgt agcgaatgat tggggattat caatccctta aagcacgccg
    15961 aagaagacga tcgtcgcgcc cgggcggtcg aaagggacat cgatgacggc gcggccggat
    16021 gcgtcgaggt gcacgcttgc gccggaagtg caccagatat gcgggtcgac acggtgttcc
    16081 gtaatatatc ggcggtggcc gccattgctg aaaaggacga cttcgccgcg gccgtcacga
    16141 acgttgatct tttctatcgt ttcgctgctg ttgcccggtt cgccttcgcg gatggggtcg
    16201 ctggcggtga cgtcgtcccg gacctgtttg taacgttcga ggatgtcgtg aataaatcgg
    16261 atgtcgtcgg gggtgttttg ccattggcca acggtgtctg ccattcggga acgatatcga
    16321 agttgtggaa ataggcgccg ttgttcagga tgatgtattt accggaagat agaaatccca
    16381 gccccatgct ccggctttct tcggtgacat ccaattcgaa aatggtgttg ggtgccgcag
    16441 cgcagacttt gtcggcaatc ttcgagagat ataccggcaa ttggaaggca tagctgtccg
    16501 cgcgctcctg cgggctattt gcttcggtgc
//
LOCUS       DASYSV010000117         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2071793, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000117 DASYSV010000000
VERSION     DASYSV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071793"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /gene="murA"
                     /locus_tag="VGQ51_03790"
     CDS             <1..1331
                     /gene="murA"
                     /locus_tag="VGQ51_03790"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV9035710.1"
                     /translation="PWRHQTQRVHSFEVIGGKKLKGEIVPQGAKNEALQIISAVLLTP
                     EKMTISNIPDILDVNLLIDLLEEMGVKIERSDRHTCIFQADEVDIGYLRSEAYQKKSG
                     RLRGSVMVAGPMLARFRKAFIPKPGGDKIGRRRLDTHIIGFEKLGANFRYEQGDGLFH
                     LEAPHLKGTHLLLDEPSVTGTANIIMAAVMAEGVTTLYNAACEPYIQQLCKMLSRMGA
                     KIHGIGSNLLHIDGVSYLGGTEHRMLPDMIEIGSFIGLAAMTQSEITIRNAGLDHLGI
                     IPEKFRQLGIRLDFRGDDIHIPEQDVYEIQSFLDGSVLTIYDHPWPGFTPDLLSIVLV
                     VATQARGSVLIHQKMFESRLFFVDKLIDMGAQIILCDPHRAAVIGLAREQRLRGITMS
                     SPDIRAGVALLIAALSAEGRSVIQNIEQIDRGYQQIDHRLRQLGAQIDRI"
     gene            1328..1903
                     /gene="gmk"
                     /locus_tag="VGQ51_03795"
     CDS             1328..1903
                     /gene="gmk"
                     /locus_tag="VGQ51_03795"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV9035711.1"
                     /translation="MTEQGKIIIITAPSGAGKTSITHWLLAKYPDKLAFSVSAATRNA
                     RSYEKDGTDYYFIPLENFKQKIQHGEFVEWEMVYEGKYYGTLKSELERIWKAGKTPLL
                     DIEVKGAIHIREQFPDSTLALFIEPPSVDELRRRLEMRGTETVESLLARVNKASYEIS
                     FKHHFDQIIVNDDLNKACLETETAIRAFLGF"
     gene            1952..>2831
                     /locus_tag="VGQ51_03800"
     CDS             1952..>2831
                     /locus_tag="VGQ51_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEV9035712.1"
                     /translation="MINTNTLVWIVIAWLMTSFFGGIGVAFARANRLAIELRKKQGLS
                     SGHILSHFMDHPTEFIGTSLGAYTVFLVIFGLMVGETFLPLWNKMITIVHMPGSYVNA
                     IRLFAETLVASLFILLFGEFIPKALFRAKSDSLLSFFAQTMDFFSRFFSPLVGFFAGI
                     AQWILKYVFNVRIDDRKPVFAEADLENFLQQTGDTEVEAQDLNKNLFEAALALPKVRV
                     RECLIPRKEVEGIDANQTMAEVRKKFVGTRLSKLVVYEGNLDHVTGYIHQLDLFRNPS
                     SIKSILHPIPAVPESMS"
BASE COUNT          677 a          764 c          767 g          623 t
ORIGIN      
        1 ggccctggcg ccatcaaacc caacgtgtgc attcattcga agtcatcggc ggcaaaaagc
       61 taaaaggtga gatcgtcccg cagggtgcga agaatgaggc cttgcagatc atcagcgccg
      121 ttctgctgac cccggagaag atgaccatct ccaatatccc cgacatattg gacgtcaacc
      181 tgctgatcga tctcctcgaa gaaatggggg tgaagatcga aaggtccgac cggcatacct
      241 gtatatttca ggccgatgag gtggatatcg ggtacctgcg aagtgaggcc tatcagaaga
      301 agagcgggcg actccgtggt tcagtaatgg tcgcggggcc gatgttagcg cgatttcgca
      361 aggcgttcat tcccaagccg ggcggcgaca agatcgggag acgccggctg gacacacata
      421 tcattggctt cgaaaagctg ggcgctaact tcaggtacga gcagggggac ggtcttttcc
      481 atctcgaggc gccgcatctg aaaggaacgc atctgttgct ggacgagcct tccgttaccg
      541 gcaccgccaa catcatcatg gctgccgtga tggccgaagg ggtcaccacc ctctacaatg
      601 ccgcatgtga gccttatatc cagcaattat gcaaaatgct gtcccggatg ggcgcgaaga
      661 tccatggcat aggcagcaat ctcctgcaca tcgatggagt atcgtatctc ggcggcaccg
      721 aacaccggat gttgcccgat atgatcgaga tcggatcttt tatcggtctc gcagccatga
      781 cgcaaagcga gatcacgatc cgaaatgccg gactggacca cctggggatc attcccgaga
      841 agttcagaca gctgggcatc cggctggatt tcaggggtga cgatattcac atacccgaac
      901 aggacgtcta tgagatacag agttttctcg acggttcggt cctgacgatc tatgaccatc
      961 cctggccggg tttcacgccc gatctgctga gtatcgtcct ggtagttgct acacaggcgc
     1021 gcggttccgt cctcatccat cagaaaatgt tcgaaagcag gctctttttt gtcgacaagc
     1081 tgatcgatat gggagcccag atcattcttt gcgaccctca tcgcgcggcc gtcatcggat
     1141 tggccaggga gcagcgatta cgcggcatta cgatgagcag tcccgatata cgggccggcg
     1201 tcgccctcct cattgccgcc ctcagcgcgg aaggacgcag cgtcattcaa aatatcgaac
     1261 agatcgacag gggttaccag cagatcgatc atcgactcag acagctgggc gcccaaattg
     1321 acagaatatg accgaacaag gcaagatcat catcattacc gcgccatcgg gtgcgggaaa
     1381 gacgtccatc acccattggc tgctggcgaa atatcctgat aaactggctt tttccgtcag
     1441 cgccgcgacc cgcaacgcaa ggtcgtatga gaaagatgga acggattatt attttatccc
     1501 gctggaaaat ttcaaacaga agatccagca tggcgagttc gtcgagtggg agatggtcta
     1561 tgaaggcaaa tactatggaa cgctaaaaag cgagctcgaa cgcatctgga aggccgggaa
     1621 gacgcccttg ctggatatcg aggtgaaagg cgccatccat attcgcgaac aatttcccga
     1681 ttcgaccctg gccctgttca tcgaaccgcc gtccgtcgat gaattgcgcc gccggctgga
     1741 aatgcgcggc accgagacgg ttgaaagcct gctggcccgc gtaaataagg cctcctacga
     1801 gatctctttc aaacaccatt tcgatcagat catcgtcaat gacgatctga ataaggcctg
     1861 tctggaaacc gagacggcga tcagggcgtt ccttggattt tagtactttc gtgaagggga
     1921 ctgcaggtcc gccggccgaa agagcatcat tatgatcaat accaatactc ttgtctggat
     1981 tgttatcgcc tggctgatga cctccttttt cggaggtatc ggggttgcat ttgcccgggc
     2041 caaccgcctt gcgatcgaat tgagaaaaaa acagggactc agcagcgggc atatcctttc
     2101 gcatttcatg gatcacccga ccgaattcat cgggacttcg ctgggagcct ataccgtatt
     2161 cctggtcatc ttcggtctga tggtaggcga gacctttctg ccgctatgga acaagatgat
     2221 aaccatcgtc catatgccgg gcagctatgt caatgcaatc cgtctttttg cagaaaccct
     2281 ggtcgcctcg ctgttcatcc tgctcttcgg tgaattcatc cctaaagcgt tgttccgcgc
     2341 caaaagcgat tcattgctgt ccttcttcgc gcaaacgatg gattttttca gccggttctt
     2401 ttcaccactg gtgggattct tcgccggtat cgcgcaatgg atcctgaaat atgtgttcaa
     2461 cgtgcgtatc gacgaccgga aacccgtatt tgcggaggcc gatctggaga attttctaca
     2521 gcaaaccggc gataccgaag tcgaagcgca ggatctgaac aaaaaccttt tcgaagcggc
     2581 gctggccctt cccaaggtga gggtacgcga atgcctgatc ccacgcaagg aggtcgaagg
     2641 catcgacgcg aaccagacca tggcggaagt cagaaagaaa ttcgtcggga cccgtctgag
     2701 caagctggtg gtctatgagg gcaacctgga tcatgtgacg ggctatatcc atcaattgga
     2761 cctgttcagg aacccctcct ccatcaagag tatcctgcat cccatcccgg ccgtgcctga
     2821 aagcatgagc g
//
LOCUS       DASYSV010000118         4788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_407051, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000118 DASYSV010000000
VERSION     DASYSV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407051"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VGQ51_03805"
     CDS             complement(<1..704)
                     /locus_tag="VGQ51_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9035713.1"
                     /translation="MQQSSALAGGAFLLPALGTGGIFSRVSPADQLNVGAIGLNGMGW
                     ADLSAAIKIPGVNVVALCDVDQNVLDRRMKELASHGVDTGRIKVYNDYRKLLDRKDID
                     FVIIGTPDHWHALIMMEACMAGKDVYVEKPVGNSIGECRAMIAAAKKYNRVVQGGQWQ
                     RSQQHFKDAVDFVYSGQLGRIRTVKVWCYQGWMKPGPVVADSAPPAGVDFAMWLGPAP
                     KRPFNSSRFHFNFRWF"
     gene            complement(812..1366)
                     /locus_tag="VGQ51_03810"
     CDS             complement(812..1366)
                     /locus_tag="VGQ51_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035714.1"
                     /translation="MHRKLTLLLFLAPLLLVISCKKSNSSGPSALVGTWNFLDMKANT
                     STRATNSGITTVTVANYTTINNAGTAKFTNDSMSVAGLTYAVNTNAETYLYQGSVLYD
                     SLSLPFSETIPPITETVKYKLIGSDSIYFPNGGFTPTGIPTGQGTGGRFTIKGDTLAI
                     NISGSQSSPIGLVTVVGAVYFIKQ"
     gene            complement(1478..2134)
                     /locus_tag="VGQ51_03815"
     CDS             complement(1478..2134)
                     /locus_tag="VGQ51_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035715.1"
                     /translation="MNKSHAYLLIVSCAALTIACNKHTSGPDAGYGPNSLFPLTNGDT
                     WYYSDSAFNDSALIAAYADTMVATKNSITDNTGTVYLELSDPYGWFNGSYIAVDPSNF
                     AVYEVDSPAYQPYTFFATVNQDGQISAPYTDGSNTACPITYAQYGYANPVNVYGFSAY
                     KNSEMVTDCHGVVLEETNSYLSPGIGVVRIEDFLTDTTGGRNLFYEDFSQTLTAKSLH
                     "
     gene            2285..3877
                     /locus_tag="VGQ51_03820"
     CDS             2285..3877
                     /locus_tag="VGQ51_03820"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV9035716.1"
                     /translation="MKQVSLIVTILFFLVGCSSVTNPNPHVSPTPYSDTSNPAARYTS
                     LTIDTTTLSRFIKNEVHDPNDARRIRAFYAGRNFQFAWFDEQGLTEQGEAYWNLHQML
                     AAKLGDTSTFERELAMDMATLTDNEGSPLSRFEWPKTELRLTSGFFRYLNRLFDTKIR
                     PEDMQWYIPRRKINPRAMLDSFLAGGRPELKPLNRFYYRLLDKMEQYIAIAKNGGWPS
                     IPHAKKALSPGSTDPIITLIKRRLQITGDLDPTDTGPLFSDSLHAAVIRMQRSFGLKE
                     TGNIDDRLIRLLNVPVEQRIETMDINLYRTRWLPEESPDFLVANIPEFRLHVFENNKL
                     AFSMRVVVGKTAHRTVIFSDSLKYIVFSPYWNVPPSIVRNEMLPAMKKDRSYLARHNL
                     EITGYNNGLPVIRQKPGKDNSLGRVKFLFPNRYNIYFHDTPAKSLFEKETRAFSHGCV
                     RLGDPVKLAVWLLRDDRSFTREKILAAMAQPKEKWVRLDHPIPVFIVYFTAWVTSDGL
                     LNFRDDIYGHDKRMARHLYADK"
     gene            complement(3983..4056)
                     /locus_tag="VGQ51_03825"
     tRNA            complement(3983..4056)
                     /locus_tag="VGQ51_03825"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4020..4022),aa:Val,seq:gac)
     gene            complement(4123..>4788)
                     /locus_tag="VGQ51_03830"
     CDS             complement(4123..>4788)
                     /locus_tag="VGQ51_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEV9035717.1"
                     /translation="RAAAERGIGVLVDAEETWIQHPVDVLTINMMDQFNKGRVVVYNT
                     IQLYRTDRLGFLKDSYEAAVQRDFVLGAKLVRGAYMEKERKRAGEMGYASPIQPDKEA
                     TDRDYNAGVAFCIGALDRIAMIVASHNENSNLLAVQLLQEKGLPLDHPHVHFSQLYGM
                     SDNITFNLAKAGCAVSKYLPFGPIKDVIPYLMRRAQENSSMSGQTGRELGLIRKELKR
                     RGA"
BASE COUNT         1161 a         1476 c         1076 g         1075 t
ORIGIN      
        1 caaaaccagc ggaaattgaa atgaaaacga ctcgaattga aaggacgctt cggcgccgga
       61 cccagccaca tggcaaagtc cactcccgct ggaggcgccg aatccgcgac gacaggcccc
      121 ggcttcatcc atccctggta gcaccatacc ttgaccgtcc tgatacggcc taactgaccc
      181 gaataaacga aatcgaccgc gtccttgaaa tgctgctggc tccgttgcca ctgtccgccc
      241 tgcactaccc ggttgtattt tttcgctgcg gctatcatgg cccggcactc ccctatggaa
      301 ttacccacgg gcttctccac gtaaacgtcc ttccccgcca tacaggcttc catcatgatc
      361 aacgcatgcc agtgatccgg cgtaccgatg atcacgaaat cgatatcctt ccgatcgagc
      421 aatttccggt agtcgttata gaccttgatc cgtcccgtat cgaccccgtg cgaagccagc
      481 tccttcattc ggcggtccag cacattttga tcgacgtcgc aaagcgcgac gacattcaca
      541 ccgggtatct ttatagccgc ggacaggtcg gcccatccca ttccattcaa cccgatcgca
      601 cccacattca gctgatcggc cggcgataca cgcgaaaata ttccgccggt cccaagggcc
      661 ggcaaaagaa aagccccgcc cgcaagggct gagctttgtt gcaaaaaagt tcgacgatta
      721 tttttcatat ggaaacagcg caactctgcg ccgatccaat atacgatctt ccccgcgccg
      781 atccaaacat acctctcacg gagccccctt cctattgttt gatgaaatag accgcgccaa
      841 ccacggtcac gagaccgatc gggctggact ggctccccga gatattgatc gcgagcgtat
      901 cccccttgat cgtgaaccgt ccccccgttc cctgacccgt cgggatcccg gtaggcgtaa
      961 agccgccatt ggggaaatag atggagtcgc tgccgatcaa tttgtacttg accgtttcgg
     1021 taatgggtgg gatcgtttcg ctgaaaggaa gactaagcga atcgtacaac acggagccct
     1081 ggtaaagata agtctcggca ttggtattga cggcgtaggt cagcccggcg accgacatgc
     1141 tgtcgttagt gaatttggcc gtaccggcat tgttgatcgt ggtataattg gccacggtga
     1201 cggtagtaat acccgagttg gtcgctctcg tggacgtgtt cgccttcata tcgaggaagt
     1261 tccaggtacc gaccagcgcg ctcggtccgg atgaattcga tttcttacag gagatcacaa
     1321 gcagcaaagg agccaaaaaa agcagtagag taagcttacg gtgcatagta taggatttca
     1381 tttgctaacg aaaaaatggc atttttagta tgccattcgc ccacctgatc gaagtattcc
     1441 cgcccgcacc gcccgccagg ctccaacgtc tcatccgtca gtgcaaactc ttcgccgtca
     1501 gcgtctgcga aaaatcttcg tagaacaaat tccttcctcc cgtagtgtcg gtgagaaaat
     1561 cctcgatcct cactacccca ataccgggac tgagatagga attcgtctct tccagcacca
     1621 cgccatgaca atcggttacc atctccgaat tcttataagc ggagaaacca tatacattca
     1681 ccgggttggc ataaccatac tgggcataag tgatcggaca ggccgtattg gaaccatccg
     1741 tatagggagc ggagatctgc ccgtcctggt tgacggtcgc aaaaaacgta tatggctgat
     1801 aggctggact atccacctca taaacggcaa aattgctcgg atctactgcg atatagcttc
     1861 cattgaacca gccatacgga tcactcagct caaggtagac cgttccggta ttgtcggtga
     1921 tggaattctt cgtcgccacc atcgtatcgg catatgcggc gatcagggcg ctgtcattga
     1981 atgccgaatc cgaataatac caggtatccc cattcgtcag gggaaacaag gaattggggc
     2041 cataccccgc atccggtccc gaggtgtgct tattacaggc gatagtcagg gcggcacagg
     2101 atacgatcaa aagatacgcg tgcgatttat tcataatgaa ggatttcggg tccgttggac
     2161 ccttaataaa actcaaaagt ttatccatta attgttaaag aatccttaac aagtctgttt
     2221 tttaacgggc aaatccggtt tttgggtccg actccaccca tgtacctttg tgtttcaaaa
     2281 ggttatgaag caggtatcac tgatcgtcac catccttttc ttcctggtcg gttgctcatc
     2341 cgtcaccaat cccaatcccc atgtctcccc gacgccttat tcggacacct ccaatcccgc
     2401 tgcccgctat acctctctga ccatcgatac tactacgctc tcccgcttta taaaaaacga
     2461 agtgcacgat ccaaacgatg cccgcaggat cagggccttc tatgccggcc ggaattttca
     2521 gttcgcatgg ttcgacgagc agggtctcac cgaacaggga gaagcctatt ggaatctcca
     2581 tcaaatgctc gcagccaaac tgggagatac ttcgacattc gaacgcgagc ttgccatgga
     2641 tatggccacg ctcacggata acgagggctc cccgctttcg cgtttcgaat ggcccaaaac
     2701 cgaactacgg ctgacttcag ggttcttcag gtatctcaac cggctcttcg acacgaagat
     2761 ccggcccgag gacatgcaat ggtatattcc ccggcgtaag atcaacccgc gggcaatgct
     2821 cgattctttc cttgccggcg gccgtcccga actcaagccg ctgaataggt tctactaccg
     2881 cctcctggat aagatggagc aatatatcgc catcgcaaaa aatggaggct ggccctccat
     2941 tccacacgcg aaaaaagcgc tctcacccgg ttcgacagac cccatcatta ccctcatcaa
     3001 acgacgactt cagatcaccg gcgatcttga ccctaccgat accggcccgc tattttcgga
     3061 tagcctgcac gcagccgtca ttcgaatgca acgctccttt ggcttgaagg agacgggcaa
     3121 tatcgacgat cgattgatca gactgctgaa cgtccccgtc gaacagcgca tcgagacaat
     3181 ggatatcaat ctttaccgta cacgatggct gcccgaagaa agccccgatt tcctcgttgc
     3241 caacatcccc gagttcaggc tccacgtctt cgaaaacaac aaactggcct tcagcatgcg
     3301 ggtcgttgtc ggaaagacgg cccacagaac cgttatcttt tccgacagcc tcaaatacat
     3361 cgtcttcagc ccctactgga atgtcccccc atcgatcgta cgcaatgaaa tgctgcccgc
     3421 gatgaaaaag gatcgctctt acctcgcccg ccacaacctc gagatcaccg gttacaacaa
     3481 cggactgccc gtcatccgtc aaaagcccgg caaagacaat tcgctgggcc gggtgaaatt
     3541 ccttttcccc aatcgttata atatctattt ccacgacact cccgccaaat cccttttcga
     3601 aaaagaaacc cgtgccttca gccacggatg cgtccgcctc ggcgaccccg tcaagctcgc
     3661 cgtctggctc ctccgggatg acaggtcctt cacccgcgaa aagatcctcg ccgccatggc
     3721 ccagcctaaa gagaaatggg tccggctcga tcaccctatc cccgtcttca tcgtttattt
     3781 cacagcctgg gtcaccagcg acggcctcct caacttccgc gatgacatct acggccatga
     3841 caaacgcatg gcccggcacc tctacgcaga caaataaaaa aagcccaagg ctcccaaagg
     3901 ccccggttcc caaaagcccc cggctccatc ggatctcccc attttcaaaa taaaaagccc
     3961 ccggcgattg ccggaggcct cgtgggtcgt acaggattcg aaccggtgac ccctaccatg
     4021 tcaaggtagt actctaacca actgagctaa cgaccctcat tccttcccgt tcgaataaac
     4081 agggcgtaaa actaaggatt ttttgcattc agcccctaaa tattatgctc ctctcctctt
     4141 cagctctttc cgtataagcc caagctccct gcccgtctgc ccggacatac tgctattctc
     4201 ctgcgcacgc cgcatcaggt aagggatcac gtcttttata ggtccgaaag gtaaatactt
     4261 gctcaccgcg caccccgcct tcgccaggtt gaacgtgata ttgtcgctca ttccgtacag
     4321 ctgagagaaa tgaacgtgcg gatggtccag cggcaagccc ttttcctgca gcagttgcac
     4381 cgccagaaga ttactatttt cgttgtgcga agccacgatc atcgcgatac gatccaacgc
     4441 gccgatacaa aaagccaccc ccgcattgta atccctgtcg gtagcttcct tatccggttg
     4501 tatcggcgac gcatatccca tctcaccggc acgcttccgc tctttttcca tgtatgcacc
     4561 gcgcaccagt ttggcgccca acacaaaatc gcgctgcaca gccgcctcat aagaatcttt
     4621 caaaaaaccc agcctgtccg tacggtacaa ttgtatcgta ttatagacga ccacacgccc
     4681 cttgttgaac tgatccatca tgtttatcgt cagcacatcc acgggatgct gtatccatgt
     4741 ctcttccgca tcgaccagaa cacctatccc ccgctcggcc gccgcccg
//
LOCUS       DASYSV010000119        13549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1175939, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000119 DASYSV010000000
VERSION     DASYSV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175939"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="VGQ51_03835"
     CDS             <1..1561
                     /locus_tag="VGQ51_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035718.1"
                     /translation="DPNNYPVGQVTLAHNQYTHQMTETMGRWTGDVGPGRLEGLLGVD
                     WQQRNTQYNSLLSSGYPNDISLNAGVGATSYIPMSNTVPYRYAALFGRANYNVANKYL
                     VTASWRRDGSSRLGNQEPYGDFWALGGAWVFSEERFLAADTGIINFGKIRASYGTTGN
                     EPNEDNQFMEVYTSSLPSRGYQGLQGLRPTTLGNQQLQWEMNYREELALEMELFHHRL
                     WFSVAGSRSWTDNQLIIMKAGPVAGINKVLMNQPGINVENRALEIELQANKVALGPVN
                     WTSAVMLTVPRNKLAAWPGLAGTSYSNTYVIGHSLTTSQNYHWTGVDPKTGYYSFQTN
                     NPNGSPGPFDVAPNSGLDPKYYAGWSNSFRMGNWQLDVLLDYRRQQGYNPLVILAQQH
                     APGSQAPLQLSNGPVEWLDHWRKTGDVTRQQRVTSGADTLALTRLYDYTASDAWSMDA
                     SYLRLRNMTLSWHLPEGMAKKWKLQGARIYVSGRNLLTVTKFPVADPETQNPTVLPPL
                     RTVIAGLRVNF"
     gene            1564..2958
                     /locus_tag="VGQ51_03840"
     CDS             1564..2958
                     /locus_tag="VGQ51_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9035719.1"
                     /translation="MKRIRKYWLGIAVLFAGWFIPTSCRKLTQTAPAADLLTVSKVFS
                     GDESAQEAMSAFYIDMVFTPRSMMNAGTSLLAGLSGDELDCQIKPYPAEDSFRLNMLN
                     ANSVISNNLFTGAYALIYELNSILEGVAHSTGMSVAVQHELKGEAEFNRAMLYFYLVN
                     LYGPVQLALKPNFQDNEQLARTSVDSVYAQLASDLEDAERLLPGDYIDAPGYTGDRTR
                     PNQAAAAALLARIRLYQGKWASADSAATAVINNSHYVLETNLDSVFLSVSREAIWQLQ
                     PVHGPVATVDASVFLTPPGKPAFVFTSGLLGAFEAGDLRRQHWTKSSLFGGVTYTYPY
                     KYKSLNAGATTEYEMVLRLAEQYLIRAEAEVQEGDVSHAIADLNVVRFRAGLPAYSMG
                     TTTAAIMAAVMQERRIELMTEWGHRWLDLKRWGLANTVLSAEKPWWQTDDALYPIPAA
                     QLQATPGMSQNAGY"
     gene            complement(2955..3335)
                     /locus_tag="VGQ51_03845"
     CDS             complement(2955..3335)
                     /locus_tag="VGQ51_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035720.1"
                     /translation="MKRLSISLPAIALVLALALSGTAFTSAPGTSNNTITTTPAQHYQ
                     NVPFLKKNYFDQSYFWYWADDDTYNDYGTVLYEEWELGQYYYPGVEIDTNPGAGTLLM
                     KGYPYYCQPHNTLPLVFLYAHFTY"
     gene            complement(3569..4834)
                     /locus_tag="VGQ51_03850"
     CDS             complement(3569..4834)
                     /locus_tag="VGQ51_03850"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEV9035721.1"
                     /translation="MPIKPMEQPGQFNIDPIAVKYAQREPLTPEEETLLREWIARGEG
                     RAQLLEQLRGGPDWADTDPTPDDKHADIRIWNKLETRLQGEGFWPDNDIAAPIPVIPA
                     RHTIPWRTAIAVAASILIVATGVLLWTTRRNTTTPTTPVAGIVSADVQPGGNRAMLTL
                     ADGSHIDLDSTANGVLSSQGNTFVAKQDGLLAYNKASSEKPAGLTYNTLTTPRAGQFK
                     LTLSDGTRVWLNNASALRYPVWFTGNTREVDLDGEAYFEVAKDAAHPFRVHIHNGVAG
                     QDGGTIDVLGTSFNIMAYDDESAERATLIDGSIRYSRAGATALLKPEEQSVLDAQGHL
                     KTLHNVNVAEITAWKNGYFHFDHANLETTMRQLARWYDVTVEYQGNIPPQEFHGRIQR
                     SMSLSAVLKGLEGDDIHFRLEGKRLTVMP"
     gene            complement(4907..5536)
                     /locus_tag="VGQ51_03855"
     CDS             complement(4907..5536)
                     /locus_tag="VGQ51_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9035722.1"
                     /translation="MQADQRSDQSIHNNEEAIMTGFREGNKDAFRMVYDQMVRPLTYF
                     VENIIRNQADAEDIVANAFYKLFHAREGMRTFEHIKRWLYVIVRNEAIDYLRSKTRVK
                     ESHHDMAYLESGVDEHVETERVRSILLQDINREIDKLPRQRKTILRLYFFEQKNTTEI
                     AELLQLSPQTVLNHKTKALDSLRKSSLRFKWLIEGVPGFILAAAFLLMK"
     gene            complement(5789..7129)
                     /locus_tag="VGQ51_03860"
     CDS             complement(5789..7129)
                     /locus_tag="VGQ51_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV9035723.1"
                     /translation="MKRFLSLILAIIPTYPVLSQPVRHVILITIDGMRPEFYEDPSWP
                     APNLQHLMHEGAYAVKLRPVFPTVTLPNHTSMITGALPARHGVYFNAPFRPLDPAPAW
                     NFYADIIKSPTLFDKIHEEKLLTAAVDWPVTVGSPSIDYNFPDYWGFDDKTDNIALIR
                     KGVHPAGLWEELEANALGKVPSEEINSDHYLRTDENAGRIAAYLIMRYKPAFTAFHIT
                     ETDHAQHDEGRESYAIRIAVAAADRAIGDVLEAVERAGIQDSTTIIIAGDHGFSDIHS
                     AIFPNVWLAKAGIFHPGNPHWRAKFQSASGSAFLYLEDKHDTAAIIAARQTLENVPSG
                     YRRLFRIIDRPELDRLGVDSSAALGFAPVIGVAVGNSGEGEPLRAAHGGTHGFMNDTP
                     EMYTGFIAYGAGIRKGTVIPMMSTPDIAPLIAKLLGIDFPCPDGILYPGLLKNK"
     gene            7216..7773
                     /locus_tag="VGQ51_03865"
     CDS             7216..7773
                     /locus_tag="VGQ51_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9035724.1"
                     /translation="MRDNHPSDSALLEGLRADSRDAFAAVYRRYCDSVYLCTRKLSGD
                     DDLASDLTQEVFKGLWDYRSQLRDARHLRSYIFFMARCHFLEHLRKVRTVVAAQEELA
                     LLSDPSEPSIELAIITEEVFAELEAALRQLSPQRKQVMQLRYFEGLDIRSIALRLDIA
                     PQTVRNHISQAIEFLRLKKILLPTS"
     gene            7850..8914
                     /locus_tag="VGQ51_03870"
     CDS             7850..8914
                     /locus_tag="VGQ51_03870"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEV9035725.1"
                     /translation="MHEPLTPEEDHLLADWRGRSPGHDELPEQLRDPEWIEEHRRLLR
                     QNPAPIVSLPVSPYRIGWRKPLLAVVCVLLVVGGWMLLRPGSSSGADDLVASGRVPAG
                     VRAYLTREDGSVLVLDTVADNTLSYVGYRGPVSRQRLVVSPGAGPWRILLADGSRVVL
                     KDGSRLEFTTDLRSGRPVLEGEAWFGVSRDASRPLTLGLAGGTEVGVLGTTFAARAAG
                     GETRVLLYSGAVRVRKGADSLLLRPGQAAVVGGSGLAARAMDDPERELAWRGRYAEEE
                     VFDFESASLAAMLKELAGWYGVRVSNPDNIQGVGMTGKLQRSKSLEDMLKALERVEKG
                     HARLRVNSDTIQVLPWHPGG"
     gene            9412..12894
                     /locus_tag="VGQ51_03875"
     CDS             9412..12894
                     /locus_tag="VGQ51_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9035726.1"
                     /translation="MSTFKDRSPFVKARPSNQPFARSAAPYPRANHIASITKTLLLAV
                     RLTGIVILIACLHASAGINAQDRISINMKSAPLEKVFAEIELRSGYTVFYNTEVLKYS
                     GPITVEMKDAMVADVMRHVLRGLPLEFNIQDKTIFVKHVKPAAVAGDPEGPGKNGATP
                     STVSGIVQNEAGQPLVGATVYIKRLKKMAVTDAKGEVSWKDVPDGEYDVDVSYVGFER
                     LTTTITVVNHEAVFTAALKQMMSKLDETVVKGYYTTTNRLNTGDVTTVKGEDIQKQPV
                     SDPILALEGRVPGMYIQQTSGIPGAYSTIMIRGQSSIANGNDPLYIVDGVPFSSVSLT
                     NTYIHGGAAGSPNPNGISNVQGGYASGGGLSPFNNLNPADIESIVVLKDADATAIYGS
                     RGANGVVLITTKKGKAGKTRFDMNVFSGGAEVTRIIHLLNTQQYLAMRREAFANDGLA
                     VPSIITNPKDNNYDIDGFWDTTRYTNWQKALIGKTANFTNAQGNLSGGNANTQFEIGG
                     GYSKQGTVYPGDYSDQKTSAHINLTHISENQRFQTQFTTNYVFDNNNIPLIDLTGRIV
                     LAPDAPSLYSANGNINWAVLNGTSTFTNPIAGILNTAKATTDNLISSLKLSYQIIPGL
                     EIKSDFGYNHTQMNQSLLTPASISNPPPFNLSSRSSNYIAVTDFKSWIIEPQIDFKHN
                     IGHGRLEALLGTSFQENNHSTSSQIAVGFTSDALISDVPAASSISLGPQGSILYHYNA
                     LYGRLNYDWEEKYLINVTARRDGSSRFGPGKQFGNFGAIGAGWIFSKESFIEDALPFL
                     SFGKLRVSYGTTGNDQIIDYQYLSTYTPSSPTYQGITGLTPSQLFNPDFAWEVVKKLE
                     GGLELGFLRDRILVTASYYRDRTGNQLVGYSLPTVTGFSSVQFNLPAVVQNSGMELTL
                     NAVNVRTKSFGWTTALNLTIPQNKLVAFPNLAKFPSYATKFVVGKSLFIQEIYHSTGV
                     NPQTGLYSFATKNTNGLPSSPQDLVITQPVTQKLYGGISNSLSYKGIQFDVLVQYVDQ
                     IGRNITSFGAAGTVNANQPITVLGRWKSIGNLTNVQRFGTSIATALNYNYFASSDGIL
                     ANTSFFRLKNVAVSYSIPANWVSKYNIKDVRIYFQCQNLFTITEDYKGLDPETGGLGL
                     PPLRTITAGIQVGF"
     gene            13130..13297
                     /locus_tag="VGQ51_03880"
     CDS             13130..13297
                     /locus_tag="VGQ51_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035727.1"
                     /translation="MIFYLMAVNIHPCPCPTEPGLSIFEVAALAAILEAVLSFVVAIN
                     KMQEKRKEKED"
BASE COUNT         3189 a         3018 c         4094 g         3248 t
ORIGIN      
        1 ggaccccaat aactatccgg tgggacaggt gacgctggcg cacaaccaat atacgcacca
       61 gatgacggaa acgatgggaa ggtggacggg tgatgtcgga ccgggtcggc tggaagggct
      121 gttgggggtt gactggcagc agaggaatac gcaatacaac tcgctcctat cgagcggtta
      181 tcccaacgat atctcgttaa atgcgggagt gggggcgact tcctacatac cgatgagcaa
      241 tacggtccct taccggtatg cggccctgtt cggaagggct aattacaatg tcgcgaataa
      301 atacctggtg acggcgtcgt ggagacggga tgggagcagc cggttgggta accaggaacc
      361 ttatggcgat ttctgggcat tgggcggggc ctgggtgttc agcgaagagc ggttcctcgc
      421 ggcggatacg ggtattatca atttcgggaa gataagggcc agctatggta cgacgggtaa
      481 tgagccgaac gaagacaatc aatttatgga agtctacacc agcagcctgc catcgagggg
      541 ataccaggga ctacagggtc tgcgaccgac gaccctgggg aatcagcagt tgcaatggga
      601 gatgaattac cgggaagagc tggcgttgga gatggaatta tttcatcacc ggttgtggtt
      661 ctctgtagcc ggctcgcgca gctggacgga caatcagctg atcatcatga aggcgggtcc
      721 tgtagcaggc atcaacaaag tcctgatgaa ccagccgggt atcaatgtcg agaaccgcgc
      781 gttggaaatc gaactgcagg cgaacaaagt tgccctgggg ccagtgaact ggacctcggc
      841 ggtgatgctg acggtaccgc gaaataaatt ggcggcgtgg ccgggtctcg ccggtaccag
      901 ctactcgaac acctatgtca tcgggcattc gctgacgaca tcgcaaaatt atcattggac
      961 gggagtggat ccgaagaccg ggtattacag ttttcaaacg aacaatccca atggatcgcc
     1021 ggggcctttc gatgtggcgc cgaattccgg gttggatccg aagtattacg ccgggtggag
     1081 caatagcttc cgtatgggca actggcagct ggatgtattg ctggattatc gccggcagca
     1141 gggttataat ccgctggtga tcctggcgca gcaacatgcg ccgggaagcc aggcgccgtt
     1201 gcaactaagc aacggaccgg tggaatggct ggatcattgg cgcaagaccg gggatgtgac
     1261 gcggcaacag cgcgtgacga gcggagcgga tacattggcg ctgaccaggc tttatgatta
     1321 tacggcctcg gatgcgtgga gcatggatgc gtcgtatctg cgtctccgga atatgacgct
     1381 aagctggcat ttgccggagg gcatggcgaa gaagtggaag ctgcaggggg ccaggatcta
     1441 tgtgagcgga aggaatttac tgacggtgac gaaattcccg gtggccgatc cggaaacgca
     1501 gaatccaacg gtgctaccgc cgctgaggac agtgatcgcg gggttgcggg ttaattttta
     1561 actatgaagc gtattcgaaa atattggttg ggtatcgcgg tgctttttgc cggatggttc
     1621 atcccgacga gctgccgcaa gctgacgcag accgcgccgg cggccgatct gctgacggtg
     1681 tccaaggttt tttccggtga tgaaagcgca caggaggcga tgagcgcctt ctatatcgat
     1741 atggttttca cgccgaggtc gatgatgaat gcgggaacgt cgctgctggc gggattgagt
     1801 ggcgacgaac tggattgcca gatcaaacct taccctgcgg aggacagttt ccgattgaac
     1861 atgctgaacg ccaacagcgt catctccaac aatcttttca ccggcgcgta tgccctgatc
     1921 tatgagttga attcgatact ggagggggtg gcgcattcga ccgggatgtc ggttgcggtt
     1981 cagcatgaat tgaagggcga ggctgaattc aatagagcca tgctgtattt ctatctagtg
     2041 aacctgtatg ggccggtgca gttggcgctg aagccaaatt tccaggataa tgaacaactg
     2101 gcgaggacgt cggtggacag tgtctatgcg cagctggcca gcgatctcga ggatgccgag
     2161 cgattgttgc cgggggatta tatcgatgcg ccgggatata cgggggacag gacgagaccc
     2221 aaccaggcgg cggccgcggc gttgctggcg aggatacgtt tgtaccaggg gaaatgggca
     2281 tcggccgaca gcgcggctac ggcggtgatc aataattcac attatgtgct cgaaacaaat
     2341 ttggacagcg tctttctttc cgtgagccgg gaggccatct ggcagctgca gccggtgcat
     2401 ggtccggtgg cgacggtcga tgcaagcgtg tttcttaccc ctccagggaa gccggcattc
     2461 gtattcactt ccggtctgct gggggccttc gaggcgggtg atctgcggcg gcagcattgg
     2521 acgaagagca gtctctttgg cggggtcacg tatacttatc cgtacaagta taaatccctg
     2581 aatgccggtg cgacgacgga atacgagatg gtgctgcggc tggcggagca atatttgatc
     2641 cgggcggagg ctgaagtgca ggaaggtgat gtgtcgcacg ctatcgccga tctcaatgta
     2701 gtgcggttca gggcagggct tcccgcttat agtatgggaa cgacgacggc ggccataatg
     2761 gccgccgtca tgcaggaacg ccgcatcgag ctgatgaccg aatggggtca tcgctggctg
     2821 gacctcaaaa ggtgggggtt agcgaacacc gtcctgtcgg cggaaaaacc ctggtggcag
     2881 acggacgatg ccctgtatcc tattccagcc gctcagttgc aggcaacgcc ggggatgtcg
     2941 caaaatgccg gttactaata agtgaaatgg gcataaagaa agaccaatgg tagggtattg
     3001 tgcggctgac agtagtaggg atatcccttc atgagcaggg tgccagctcc cggattcgtg
     3061 tcgatctcga cgcccgggta gtaatattgt cccaattccc attcttcata taagaccgta
     3121 ccgtagtcgt tgtacgtgtc gtcatcagcc cagtaccaaa aataggattg atcgaagtaa
     3181 ttctttttga gaaagggaac attctgatag tgctgggccg gagtggtcgt gatcgtgttg
     3241 ttcgatgtgc cgggagcgga agtgaaggcc gtaccggaca aagcgagggc caataccagc
     3301 gcaatggccg gcaggctgat ggataagcgt ttcattgatg ttgccgcgtt ttgggtcaaa
     3361 atgcggtcaa aactttagat taattaaata ggattccgat taccttatag ggtaataata
     3421 atgagaacaa tcaatctgtg gtataagtaa attcctatag gagatgaatg caggtaaaca
     3481 cggagctcac ggacaaatgt agaaagctgc gatgcaatca ggctgcggtg aaaagaattt
     3541 tttggaggaa aaaaaggaaa gatcaggttc agggcattac cgtgaggcgt ttgccttcga
     3601 gcctgaagtg aatgtcatcg ccctcgagac ctttgaggac ggcggacagc gacatgctgc
     3661 gttggatgcg gccgtggaac tcctgcggtg ggatattgcc ctgatattcg acggtgacgt
     3721 cgtaccagcg ggcgagctgg cgcatagtcg tttcgaggtt ggcgtggtcg aagtggaaat
     3781 agccattttt ccaggccgtg atctcggcga cgttgacgtt gtggagggtc ttaagatgac
     3841 cctgcgcgtc gaggacggat tgttcttcgg gtttgaggag tgcggttgcg cctgcgcggg
     3901 agtagcggat gctgccgtcg atgagggtgg cgcgttcggc gctctcatcg tcgtaggcca
     3961 tgatattgaa tgaggtgccg aggacgtcga tggtgccgcc gtcctggccg gcgacgccgt
     4021 tgtggatgtg gacgcggaag gggtgggcgg cgtctttggc gacctcgaaa taggcttcgc
     4081 cgtcgaggtc gacctcgcgg gtgttgccgg tgaaccagac gggatagcgg agcgcggagg
     4141 cgttgttgag ccagacgcgg gtgccgtcgg agagggtcaa tttgaactgg cctgcgcggg
     4201 gggtggtgag cgtattatag gtgaggcccg cgggtttttc ggatgaggcc ttgttatagg
     4261 ccagcagacc gtcctgtttg gcgacgaagg tattgccctg tgagctgagc acgccgttgg
     4321 cggtggaatc gagatcgata tggctgccgt cggcgagggt gagcatggcg cggttgccgc
     4381 cgggttggac gtcggcggag acgatgccgg cgacgggggt ggtgggcgtt gtggtattgc
     4441 ggcgggtggt ccataggagg acgccggtag ctacgatcaa tatggaggcc gctactgcaa
     4501 tggcggtgcg ccaggggatc gtgtgccggg ccggtatgac ggggatgggg gcggcgatgt
     4561 cgttatcggg ccagaagcct tcgccctgca ggcgggtttc gagcttgttc cagatgcgga
     4621 tgtcggcgtg tttgtcatcc ggggtggggt cggtatcagc ccaatcggga ccgccgcgga
     4681 gctgttcgag aagttgggca cgtccttcgc cacgggcgat ccattcgcgg aggagggttt
     4741 cctcttccgg cgtcagcggc tcacgctgtg cgtatttgac ggcgataggg tcgatattga
     4801 actgtcctgg ttgctccatt ggtttgattg gcattagccc ctataatagt tatacacggg
     4861 atagttgaat ttttaactat ccccgtccgg ttaagatttt attaatttat ttcattagga
     4921 ggaaggcggc cgcgaggata aacccgggaa cgccttcgat gagccatttg aatcgaaggc
     4981 tgctttttct taaggaatcc agggctttgg tcttatggtt tagaacggtc tgcgggctga
     5041 gctgcagcag ctcggcgatt tccgtcgtat ttttttgttc gaagaaatag agccgaagga
     5101 tggtcttgcg ttggcggggc agtttgtcga tctcgcggtt gatgtcctgg agaaggatgg
     5161 atctgaccct ttcggtctcg acgtgttcgt cgacgccgga ttcgagatag gccatatcgt
     5221 gatggctttc cttgactcgt gttttggagc ggaggtagtc gatggcttcg ttacggacga
     5281 tgacgtatag ccagcgtttg atgtgttcga aggtgcgcat gccttcgcgc gcatggaaga
     5341 gtttgtagaa ggcgttggcg acgatgtctt cggcgtcggc ctggttgcgg atgatgttct
     5401 cgacgaaata ggtgaggggt cggaccatct ggtcatatac catgcggaag gcgtctttat
     5461 tgccctcgcg gaacccagtc atgatggctt cttcgttgtt gtgtatcgac tgatcagatc
     5521 gttggtctgc ctgcattcgg ggtttatgaa tacacggatt tataaatagt tacgaaaaca
     5581 ccggcattga ggacaggata aaaatttaac gtgtcggcgg gaaagttgcc gaatcgatgc
     5641 aatgatagac gtttaaggtc tcatgcaact aatgaggaaa agttcgatgg gaatggttgg
     5701 gcagctgatt ggaccgcagt ttgcctgcac taaggtaata aatttatttt aggaaagaag
     5761 tatcctattt aatttttgaa gtgtctcctc atttattttt caacaatccg ggatagagga
     5821 tgccgtcggg gcaggggaaa tcgatgccca gcaattttgc gatgagcggc gcgatgtcgg
     5881 gtgtgctcat catggggatg acggtgcctt tgcggatgcc ggcaccatag gcgatgaagc
     5941 cggtgtacat ctcgggcgtg tcgttcatga agccatgtgt accgccgtgt gcggcgcgga
     6001 ggggttctcc ttcgccgctg ttgccgacgg cgactccgat gacgggtgcg aagcccagtg
     6061 ccgcggagga atcgacgccg agccgatcga gctcgggacg gtcgatgatg cgaaagaggc
     6121 ggcgataacc cgaggggacg ttttccagcg tttgacgagc ggctatgata gccgcggtat
     6181 cgtgcttatc ttcgagatag agaaaggcgg agccgctggc cgactggaat ttggcgcgcc
     6241 agtgcgggtt gccaggatga aagatcccgg cctttgcgag ccagacgttg gggaagatgg
     6301 cggaatggat atcgctgaat ccgtggtcgc cggcgatgat gatcgtcgtg ctgtcctgta
     6361 ttcccgcgcg ttcgacggct tcgaggacgt ccccgatggc gcggtcggct gccgcgacgg
     6421 cgatgcggat ggcgtaggac tcgcggcctt cgtcatgctg ggcgtggtcg gtctcggtga
     6481 tgtggaaggc ggtgaatgcg ggtttataac gcatgatgag ataagccgcg atgcggccgg
     6541 cattctcgtc ggtgcggagg tagtggtcgc tgttgatctc ttcggagggg accttgccga
     6601 gggcattggc ttccagctct tcccacagac cggcgggatg gacgcctttg cggatgaggg
     6661 cgatgttgtc cgtcttgtcg tcgaagcccc agtagtccgg gaaattgtag tcaatcgacg
     6721 gagaaccaac ggtcacgggc caatccaccg cggccgttaa caatttttct tcatgtattt
     6781 tatcgaagag agtaggtgat ttgatgatat cggcgtagaa attccacgcg ggtgcaggat
     6841 ccagggggcg gaagggggcg ttgaagtaga ccccgtggcg tgcgggaagc gcaccggtga
     6901 tcatagaggt gtggttgggg agggtgacgg tggggaagac ggggcgtaat tttaccgcat
     6961 aggcgccttc gtgcatgagg tgctgcagat tgggggcggg ccaggagggg tcttcataga
     7021 attccggccg catcccgtcg atggtgatga ggatgacgtg gcggacgggt tgcgaaagga
     7081 cggggtaagt ggggataatt gccagaatga gggaaagaaa gcgtttcatg atacgtacaa
     7141 ttataaatag gtgtgatatt tttgccatag ctaacgagcc agtgtggaaa ataaccatta
     7201 acgaaaccga agtcaatgcg tgataaccat ccaagtgatt ctgccttgct ggaaggcctc
     7261 agggccgact cgagggatgc gtttgcggcc gtgtaccgtc gttattgcga cagcgtctat
     7321 ctgtgtacac gcaaattgag cggtgatgac gacctggcca gcgatctgac gcaggaggtg
     7381 ttcaagggtc tttgggatta ccggtcgcag ctgagggatg cgcgtcatct ccgcagctat
     7441 atctttttta tggcgcggtg tcatttcctg gagcatctcc ggaaggtgcg cacggtggtc
     7501 gccgcgcagg aagagctggc gttgttgtcg gatccttccg agccctctat agaattggcg
     7561 atcatcacgg aggaggtctt tgccgagctg gaggccgcgc tccgtcagtt gtccccgcag
     7621 cgcaagcagg tgatgcagct gcgttatttc gaaggactgg atatccgatc gatcgctttg
     7681 cgactggata tcgccccgca gaccgtgcgg aatcatatct cgcaggctat agaattcctg
     7741 cgtctgaaaa aaattcttct ccccactagt taaacggaag gttttttgtc tgatcatagc
     7801 gatgaccgaa gatcagaacc gaattgtcga aatcctggta aagtatgcct tgcatgagcc
     7861 tctgacgccg gaggaggacc atctgcttgc cgactggcga ggacggtcgc cgggtcatga
     7921 tgagctgccg gagcagttga gggatccgga atggatcgag gaacatcgtc ggttgcttcg
     7981 tcagaatcct gcacctattg tttcacttcc tgtttctcct tatcggatcg gctggcgcaa
     8041 gccgctgctg gcggtggttt gcgtgctgtt ggtcgtgggc gggtggatgt tgttgcggcc
     8101 ggggagttct tcgggggcgg atgatcttgt tgcgagcgga cgcgtgccgg cgggagttcg
     8161 ggcttatctg acccgggagg acgggagtgt gcttgttctg gacacagtgg ctgataacac
     8221 attgagttac gttggatata gaggaccggt ttcgcgtcag cgtctggtgg tctcaccagg
     8281 ggcgggtcca tggcggatcc tgctggcgga tggtagccgg gttgtattga aggacgggtc
     8341 gcgtttggag ttcactaccg atctgcgcag cgggcggccg gtattggaag gcgaggcctg
     8401 gttcggagtg tcgcgggatg cgtcacgacc attgacattg ggcctcgccg gagggacgga
     8461 ggtgggggta ttggggacga ccttcgcggc gcgggctgcc ggcggagaga cgagggtgct
     8521 gctttattcg ggagcggtgc gggtgaggaa gggggccgac agcctgctgt tgaggccggg
     8581 tcaggcggcg gtggtcggcg gttcggggct ggccgcgcgg gcgatggatg atcccgagcg
     8641 ggagctggcg tggaggggac gatatgcgga ggaggaggtc ttcgatttcg agagtgcgag
     8701 cctggcggcg atgttgaagg agctggccgg gtggtatgga gtgcgggtat ccaaccccga
     8761 caatattcaa ggtgttggta tgaccggcaa gctgcagcgc agcaagtcgt tagaagatat
     8821 gttaaaggcc ctcgaacggg tagagaaggg ccacgcccgg cttcgggtga attcagacac
     8881 gatccaagta ctaccctggc atcctggggg atgaaataat catcagccat acaccattat
     8941 cccagcctat tagtaatgaa gcgccggtgg tcgagagatc gtcggcgttt tgatttatgc
     9001 gtgccaatga gggacttttg cgttttaatt aattattaat aaaaaaaatt ataaaagtcc
     9061 ttagttaact aaggacaata tcccgtagtt aatccgattg cgaaaatcaa actttacaat
     9121 tgcccttgct ttagatacca gtttacctgg gctaagatct aaccacatcc tttaggcaat
     9181 taaaccttcc tgttccgtta attcaattcg cgcaaatcat cggttaagca ccttcgggtg
     9241 acgtttggtt tattgacttt tttgacccta aaaaaggaac taacatgctc agattcagac
     9301 aaatcctgac tcctccgcca tggaggaaag ccagcccacc ctggcgcgcc gcgacatctc
     9361 ttcccgccgc gtattcaccc catcgactcc aacacttata atccataaaa tatgtctact
     9421 tttaaggacc ggtcgccctt tgtcaaggcg cgaccttcca accaaccatt tgcccgatcc
     9481 gcggcgcctt atccccgagc gaaccatatt gcatctatca ccaaaaccct gctacttgca
     9541 gtgcgactaa cggggatcgt catacttatc gcctgtctcc atgcgagcgc cggcatcaac
     9601 gcgcaggacc gcatctccat taatatgaag agcgccccgc tggaaaaggt cttcgccgag
     9661 atcgaacttc ggtcgggcta taccgtgttt tataatacgg aagtcctgaa gtactccggg
     9721 ccaatcaccg tcgagatgaa ggacgccatg gtcgccgacg tgatgcgcca cgtgctccgc
     9781 ggtctgccgc tcgagttcaa catccaggac aagaccatct tcgtcaaaca cgtcaaacct
     9841 gctgctgtcg ccggtgatcc cgaggggccc ggcaagaacg gggcgacgcc atcgaccgta
     9901 tccggcatcg tacagaacga ggccggtcag ccgctggtgg gcgcgaccgt ctatatcaag
     9961 cggctgaaga agatggcagt gacggatgcg aagggagagg tttcgtggaa ggatgtaccg
    10021 gatggggaat atgatgttga tgtatcgtat gtggggttcg agcggcttac gacgacgata
    10081 acggtggtga atcatgaggc ggtgtttacg gcggcgctga aacagatgat gagcaagttg
    10141 gatgagacgg tggtgaaggg gtattatacg acgacgaata gactgaatac gggggatgtg
    10201 acgacggtga aaggggagga tatacagaag cagccggtga gcgatccgat attggcgctg
    10261 gaggggaggg tgcctggaat gtatatacaa cagacgtccg gaattcctgg cgcatatagt
    10321 actatcatga tccgaggaca aagtagtata gcaaatggca atgatccatt atacattgtg
    10381 gatggtgtgc cgtttagttc tgtatcacta acaaacactt atattcacgg cggcgcggct
    10441 ggttctccga atccaaacgg tataagtaac gtacagggcg gatatgcgag cggcggcggc
    10501 ttgagcccat tcaataattt gaatcctgca gatatcgaaa gcatagtggt cttgaaagat
    10561 gccgatgcga ctgcaatata tgggtcgagg ggtgcgaatg gggtagtgtt aattactaca
    10621 aagaaaggaa aggcgggaaa gactaggttt gatatgaatg tgttttctgg cggtgccgaa
    10681 gtcacaagaa taatacacct tttgaatacc caacaatatc tggcaatgcg tcgggaggca
    10741 tttgcaaatg atggcttggc tgtaccatcc atcattacaa atcctaaaga taacaattac
    10801 gacatcgacg ggttctggga tacaacgaga tatacaaatt ggcaaaaggc attgattggt
    10861 aaaaccgcta attttacaaa tgcacaaggg aatttgtcag gcggtaatgc caacactcaa
    10921 tttgaaatcg gaggcggata tagtaagcaa ggcacagtat acccagggga ttattcagat
    10981 caaaaaacct cggcgcacat taatttaacg cacatttcag aaaatcaacg atttcagaca
    11041 caatttacga ccaattacgt atttgacaac aataacatac ctcttattga tctcacggga
    11101 cgaattgtgc ttgctccgga tgcgccgtcg ctatatagcg ccaatggtaa tataaactgg
    11161 gcggttttga acggcacctc cacattcaca aaccctatag cagggattct aaataccgca
    11221 aaggctacta cggacaatct tatctctagc ctcaaattgt cgtatcaaat tattccaggg
    11281 ctggagatta aaagtgattt cggctacaac catacacaga tgaaccaatc tcttttaaca
    11341 cctgcaagta tctccaatcc cccccccttt aatttatcaa gcagatcttc aaattatatc
    11401 gctgttacag attttaaatc atggataata gagccacaga ttgattttaa gcacaatatt
    11461 ggacatggaa ggttggaagc actcttggga acgagttttc aagaaaataa tcactctact
    11521 tcttcgcaaa tcgctgtagg gtttacaagt gatgcattga tttccgatgt gccagctgct
    11581 tcgtcaattt cgttgggtcc tcaaggtagt atattgtatc actataatgc tctatatggc
    11641 cgtctgaatt atgactggga ggaaaaatac cttataaacg ttacagctcg cagagatgga
    11701 agcagtcgct tcggtcctgg aaaacaattt ggaaattttg gcgcaatcgg tgcaggatgg
    11761 attttttcaa aagaatcatt tattgaagac gctttaccct ttctcagttt tggaaaatta
    11821 agagttagct acggtactac cggcaacgac caaattatag actatcaata tctgagcacc
    11881 tacaccccca gctcgcccac ctaccaaggc ataactggct taacgccatc tcagctattt
    11941 aaccctgatt ttgcctggga ggttgtaaag aaattggagg gtggacttga acttggcttt
    12001 ctaagggacc ggatacttgt tactgccagc tattaccgtg atcgtaccgg caatcaatta
    12061 gtaggttatt ccttgccaac ggttactggt ttctcatctg ttcagttcaa tcttcctgcc
    12121 gttgtacaaa actcaggcat ggagttaact ctgaatgcag ttaatgttag aacaaagagc
    12181 ttcggctgga cgactgcctt gaacttgaca ataccgcaaa ataagcttgt ggcatttcct
    12241 aacctggcta aatttccttc ttatgcaacg aaatttgtgg tgggtaaatc tttattcatt
    12301 caggagatat atcactccac tggtgtaaac ccccaaactg gtctttatag ctttgccact
    12361 aaaaacacaa acggcctacc ctctagcccc caggacttgg taataacaca gccagtaact
    12421 caaaagctgt atgggggcat ttcgaacagc ttgtcatata aagggattca attcgatgtt
    12481 ttagtacaat atgtcgatca aattgggcgc aatatcactt cttttggtgc tgccggtacg
    12541 gttaatgcca atcagccaat aacagttctg ggtcgctgga aatccattgg caatcttacg
    12601 aatgtccaac gattcggcac aagcatcgca acagctctta attataacta ttttgcaagt
    12661 agtgatggga ttctcgcaaa tacctcattc tttcgtttga aaaacgttgc agtttcgtac
    12721 tcgattcctg cgaactgggt gtccaaatat aatatcaaag acgttcgcat ttattttcaa
    12781 tgtcaaaatt tatttacaat caccgaggac tataagggcc ttgatccgga aactggggga
    12841 ttgggtcttc ctccattgag gacaattaca gccggaattc aggtcgggtt ttgatttagg
    12901 ttcgatggta aagcatcata ctaggccgta tccatggagt tatggggttc tgttgttggt
    12961 cttccgcgaa aagaaggtta ccctgttgtt aaacagacaa acccagcaca tttgaattag
    13021 gctaataagg aaagaccaaa caacggtgga acaattgtgg cgaaccccat aaaaaatacg
    13081 acaagtgtaa aatgtgttag cggtatgaat caataaaatg accaaaataa tgatcttcta
    13141 ccttatggca gttaatattc atccatgtcc ttgtccgaca gaaccgggat tatcgatctt
    13201 tgaagttgcc gcattggcgg ctattcttga ggcggtgctg tctttcgttg tagcgataaa
    13261 taagatgcaa gaaaagagaa aggaaaaaga agattagcgt ttgcagtttc cggaggcttt
    13321 agtgactcgc aaccggaaaa gactgggtta aatggatatt gagtaaacta tatttgaaaa
    13381 caatattttc taacaaaaaa accggcaaag ccacgtacat acctgtactt cgtcgggtgt
    13441 ttcacattca aatggatata aaatgctaag caaatctatt tacaaaaatc aggttgccat
    13501 aattttgggc atcgggctgt cgacgatttg tttcagttgc aaaaaattt
//
LOCUS       DASYSV010000120         7896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2329320, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000120 DASYSV010000000
VERSION     DASYSV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329320"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VGQ51_03885"
     CDS             complement(<1..411)
                     /locus_tag="VGQ51_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035728.1"
                     /translation="MLPIYKAKIFNGIIEKGGRTKPWSVLVDTGSGAKQFVVKMFPAQ
                     LVHARDSVTNEVLGYALAKEFDLPVPDAALIDMDMDFQMTINDGLAQMAFDQADDRLK
                     FGTEVIEGNYLFNSSFTRAQASRMIDVDTLYGFDN"
     gene            complement(509..709)
                     /locus_tag="VGQ51_03890"
     CDS             complement(509..709)
                     /locus_tag="VGQ51_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035729.1"
                     /translation="MIDKASLSAEWLAGKKQRYKMIQLLPEKGGTREFAHPPQSVADE
                     KNALLMLNIAMVFFQHCLAPVS"
     gene            complement(702..1508)
                     /locus_tag="VGQ51_03895"
     CDS             complement(702..1508)
                     /locus_tag="VGQ51_03895"
                     /inference="COORDINATES: protein motif:HMM:NF042521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6577 family protein"
                     /protein_id="HEV9035730.1"
                     /translation="MPIAVQCLIIFAYKTANLIRDYLIEQLKQEFGGRKSFSRQELLA
                     FFRRFEPDVKESTFRWRVYNLKHKNIISPISRQKFVITDKPAFAVSTEKIDEKLTALI
                     RKQFPHLRFAVWSTKAVNEFMLHQPARYYTLVEVEREATEAVFNFMKDNRVKNVFLEP
                     NEKELQKYISDLENAVVVLPLVSKAPIQTKRDKASITIEKLLIDLYSDRQLFNAYQGS
                     ELVNIYNTAYNRYPIDFTKLFSYATRRRKAADLKHFLSQKTDIPQNLIYD"
     gene            complement(1604..2971)
                     /locus_tag="VGQ51_03900"
     CDS             complement(1604..2971)
                     /locus_tag="VGQ51_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV9035731.1"
                     /translation="MPASLLVILDTRRIKQKINKYPVKLRVTSGRRTKNYATIYDMSE
                     EEFGKLNASNLGATLKVIRNKLRDMEREADNVIRAMTCFHFEDFEREYISNHPLFNPK
                     KSALNATVKGNYGYDAKLYAHRFPIFKELTMPAGTLLVAFRSYIDKLLRGDRIGTAVC
                     YQTSYYALEKFRGNVLFSDVTVDYLNDFEKWMLRKNISKTTIGIYVRCLRCIFNEAIE
                     DGIIKRDRYPFGRRRYQVPTSRNVKKALTLEDVSKLYYFQPTRKEEQWAKDFWFFSYL
                     GNGINPKDIAYLKYKNIEGDYFVFERAKTENATRNDPKPITVFITDDMRAIIDRWSNK
                     DKRPNNYVFPVLEPDINALRQYELIELFTQSINDWLLKIKKKLKIEKRLTTYVACHTF
                     STVLKRSGVSTEFIQESLGHMNIRTTEKYLDSFAKEVKKELVGSLTAFKAASPTTANE
                     FEESV"
     gene            complement(3293..3754)
                     /locus_tag="VGQ51_03905"
     CDS             complement(3293..3754)
                     /locus_tag="VGQ51_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEV9035732.1"
                     /translation="MEALASKETAGVLEDLIRINNDRIEGYEKALKDLKPEDSDLQLV
                     FLKAIDQSRAIRMRLGTELQTLKTDVPTGTSGSGSIHRAWLDLKATFTGHNRHAILAS
                     CEFGEDAAQKAYESALSGEHLPEYLSTLVLQEKEELKMVHDEIRALRDQSK"
     gene            3951..4304
                     /locus_tag="VGQ51_03910"
     CDS             3951..4304
                     /locus_tag="VGQ51_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035733.1"
                     /translation="MSTGGSTSDWSHSFIVECENKQRKAGFFGQHLRKYIQSDTAAVR
                     YLNQYAAKQSFKLATSVSTVVLFSIFGISNLAQKNTPENPDPPTVNMGRKTPGLQVLI
                     SECKMLGRPGRSLWE"
     gene            4619..7303
                     /locus_tag="VGQ51_03915"
     CDS             4619..7303
                     /locus_tag="VGQ51_03915"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV9035734.1"
                     /translation="MKHPSITSTDRFLLISFILCITGSLLIYQQINRQYTRSNLVTHS
                     YIIKVTLNQFLLNLTEAETNQRGYLLSHDSDFLNDYQRAIRKARFIVGILDSLTIDDP
                     GQQEGLRKLRQAFNNSMLTLQASMMLPTESFSDPHSDEIIQHTKDVLDNLRGNAYTML
                     DAEDSLLRNRVVARNNLAKRNPWYMLTTGLLVTSLIFFVYLKLKKETLLRLDSEHNTA
                     ELEKKDLLRSRELASLNEQLTTQNNVFLHAEKVALIGSYTWNLSTGQMTFSDNLFRLL
                     GYDPGEFTPSHEHLLRLIHPAERSEILKNTPFLLESIIQTETPCRFITKEGTIRYFRA
                     TGKHLYKPDGEVIAGTFQDITKDIELRQALENNDNFIRSLLDIAPSTITIFDLEKSTT
                     VFSSHPQLPTLGHRVDISGPNDNPYSLMHPDDREPAAQYLESLRHLQDREVSEFEYRM
                     KNDEGGYTYFMSRNTPYKANSRHEIIQILGATIDITEKVRAQLEVKAKTEALLRSSFE
                     QQSAIRFKTLADSMPQIVWTADAGGRIDYFNQRWYSYLRLPDKTLSTRDITDNMHPED
                     VADYFRNWNISVETGNPFQVEFRFRDQNNPGQYCWFLAKALPITDANAVIVKWHGTFT
                     DINDLKLAQLSIQQLVKEKEDFIHIASHELKTPVTSLKGSIQLLERMSGEGALAEEMD
                     VLIEMAVRQVNKLSRMVGDLLDVARMDADKIELVGRDFELQEAIADSLEQVRFLAPGS
                     TIRIDGPTDIRIHSNKERIEQVITNFLSNAIKYSPEGAEIVLEPSLLDGQLRISVTDQ
                     GIGIPSSKLPFVFDRFFRVEGSSDKFSGLGLGLYISAGIVSRLRGEIGAVSELGHGST
                     FWFTLPVNSHAQNGEPQDIIHSAKPSPDWLWQHAPPAS"
     gene            complement(7242..>7896)
                     /locus_tag="VGQ51_03920"
     CDS             complement(7242..>7896)
                     /locus_tag="VGQ51_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9035735.1"
                     /translation="KQIKNYDKTWDVIGVINDYHQKSLRYPVEPTMLMPAYSNNSIIS
                     VKVNTSDMPATIGAIKKTYDAFFPGNYFDYYFLDDHYNQQYASDRLFGKAFSIFAGFA
                     IFIACLGLLGLSLFATMQRTKEIGVRKVLGASVAHIIVLLSADFIRLVIVSILIATPL
                     AWYIMHRWLQDFAYRIDIGWWVFVVAGLLAVVIALATISWQAARAAIANPVKALRSE"
BASE COUNT         1976 a         2019 c         1914 g         1987 t
ORIGIN      
        1 gttatcaaaa ccgtacaagg tatccacgtc aatcattctg gaggcttgag cccgggtaaa
       61 agaggagttg aacagataat tgccctcaat gacttccgtg ccgaatttca aacggtcatc
      121 agcctgatca aatgccatct gcgccagccc atcgttgatg gtcatttgaa agtccatatc
      181 catgtcaatg agcgccgcgt caggtaccgg caaatcgaat tccttggcta gagcgtagcc
      241 cagcacctcg ttggtgacgg aatctctggc gtgtacgagt tgggcaggga acattttcac
      301 gacaaattgt tttgcaccgc tgcccgtgtc cacaagcaca gaccaaggct tggtccggcc
      361 gcctttttcg atgatcccgt taaatatttt ggctttgtaa ataggaagca tcgactgtat
      421 gtccagggta tatcccaaat gaccaaacga aggtaaggat tactgctttt ggcaccgcct
      481 tttacgcgat ttcccaaata attcggattc acgaaaccgg cgcgaggcag tgctgaaaaa
      541 agaccattgc gatgttcagc atgagcaaag cgtttttttc gtcggccacc gactgaggcg
      601 gatgcgcgaa ctcccttgtt cctcctttct cgggcaacaa ttggatcatt ttgtatcgtt
      661 gcttcttccc tgctaaccat tcagccgata gggatgcttt gtcaatcata gatcaggttt
      721 tgaggtatat ccgttttctg ggacaagaaa tgtttcaaat ccgcggcttt cctcctacgc
      781 gtggcatagc tgaaaagctt ggtgaaatca atgggatatc gattatacgc ggtattgtaa
      841 atgttaacca attcgcttcc ctgataggca ttgaacagtt gtctgtcgct gtagaggtct
      901 atgagtagtt tttcaatggt tatactcgcc ttatcccgct ttgtttggat aggcgccttt
      961 gacaccaagg gaagaacgac caccgcattc tccaggtcgg agatatattt ttgaagttct
     1021 ttttcattgg gttccaggaa cacgttcttc acgcggttat ccttcatgaa attgaaaaca
     1081 gcctcagtcg cctccctttc tacttcgaca agggtgtaat atctggcggg ctggtgcagc
     1141 ataaattcat tgaccgcctt tgttgaccac acggcaaagc gtaaatgggg gaattgtttc
     1201 cggatcaatg cggttagctt ttcgtcgatc ttttcagttg atactgcgaa tgccggttta
     1261 tccgttatga caaacttctg ccgggaaatg gggctgatga tatttttatg cttgagatta
     1321 taaactcgcc agcgaaaggt actttctttc acgtctggct caaaccggcg aaagaaagcc
     1381 aatagctcct gccggctgaa ggacttcctt ccgccaaatt cttgctttaa ctgttctatg
     1441 aggtagtcgc ggatcaaatt agctgtttta taggcaaata taatcaaaca ttggacagca
     1501 ataggcataa agaggcaaat atttgaaatc aaatattgga cagcaaatag cttcttgagg
     1561 caattgtttg aatccatata ttttataatc agatagttat gaattacacg ctttcctcaa
     1621 actcattggc tgtagtcggg gatgcagctt tgaaagccgt caaactaccc accagctcct
     1681 tttttacctc ctttgcaaag ctgtcgaggt atttctctgt ggtccggata ttcatgtgcc
     1741 ctagcgactc ctggataaat tcggtgctga caccggaacg tttcaggacg gtggagaagg
     1801 tatggcaggc aacataggtc gtcagccttt tttcgatttt cagtttcttt ttgatcttca
     1861 acaaccagtc attgatcgat tgcgtgaaaa gctcgatcag ctcgtattgt cttagggcat
     1921 tgatatccgg ctccagaacg gggaaaacgt agttgttcgg acgtttatcc ttattagacc
     1981 atcgatcgat aattgcccgc atgtcatccg taataaaaac agtgataggc ttcgggtcgt
     2041 tccgggtggc gttttcggtt ttagcccgct caaagacgaa ataatcgcct tcgatattct
     2101 tatacttcag ataggcgata tccttcggat tgatgccatt gccgagataa gaaaagaacc
     2161 agaagtcctt cgcccattgc tcttccttgc gggtaggttg gaaataataa agctttgaga
     2221 catcctccag cgtgagcgcc tttttgacat tcctggacgt cggaacctga tacctgcggc
     2281 ggccaaaggg gtatctgtcc cgcttaatga tgccgtcctc gatcgcttca ttgaagatgc
     2341 accgcagaca cctgacatag atcccgatag tagttttgga gatattcttc cgaagcatcc
     2401 atttttcaaa gtcgttcaga taatcgaccg tgacgtccga aaagagaaca tttccgcgga
     2461 acttctccag ggcgtagtag ctcgtctgat aacatacagc ggtgccgatc cgatcgcccc
     2521 ggagcagttt atcaatgtag gagcgaaagg caaccaggag ggtcccggcc ggcatggtca
     2581 attctttgaa gatgggaaat cggtgagcat atagcttggc atcgtagcca tagttaccct
     2641 taacagttgc gttgagggca gacttcttag gattgaaaag gggatggttt gaaatgtatt
     2701 ccctttcgaa atcttcgaag tggaagcagg tcatcgccct gatgacattg tccgcctccc
     2761 gctccatgtc cctaagctta ttccgaatga ccttgagcgt cgcgcccaaa ttcgacgcat
     2821 tcaattttcc gaattcttct tcggacatgt cgtagatggt ggcgtaattt tttgttcgcc
     2881 ttccggaggt gacccgaagt tttacggggt acttgttgat cttttgtttg atcctgcgtg
     2941 tgtccaaaat gactaataat gaggctggca taactgaaag atttttgttt gtttgcaaat
     3001 cctacgtttt gccaattatt tgcaatcaaa cagcaggtca aacgaccttt aacggggtcg
     3061 tccgaagtta gcctaaaatt gtaaacgttt gaaatttaaa cagttaagtt aaaacagctt
     3121 cacctgaaag cactctgatt aatagtcaga gggtctgtta atcagagggt cgttggttca
     3181 agtccaacag ggggagcaaa agatcaagca cctggcagat gaatgtcagg tgcttttttc
     3241 atttaaggcg actggacgga aacggacgga acgcagtact aaaaaacgcc gatcatttgc
     3301 tttgatcccg gagtgcccgg atctcatcat ggaccatctt caactcttct ttttcctgta
     3361 gaacgagggt ggaaaggtat tcgggtaaat gttcgccgga aagcgcggat tcataggcct
     3421 tttgcgcggc atcttcccca aattcgcaac tggccagtat cgcatggcgg ttatgtccgg
     3481 tgaaggtcgc cttcaaatcc agccaggcgc gatggatcga accgcttccg gaggtaccgg
     3541 tgggaacatc tgttttcagc gtttgtagtt ccgtgcctaa tctcatccgg atagcccggc
     3601 tttgatcgat ggctttcagg aagaccagct gcagatcgga atcctccggt ttcaggtctt
     3661 tcagggcctt ctcatatccc tcgatccgat cgttattgat ccttatcaga tcttccaata
     3721 cgcccgccgt ttctttagat gctaatgctt ccatatgttg ttttatggta ccgtacaaaa
     3781 aaatcatgcc agcgtcttat attcgcaaaa gccccaaaac ctaagatact aggactcatg
     3841 ctatccgact gttcctggca attgtcctga catttccact caacacttat tgttccgccc
     3901 agcagctgcc gcacatcacg ctgatccaaa aaaactatgt gtataccaat atgtcaaccg
     3961 gcgggtccac gtccgattgg agtcattcgt ttatcgtcga atgcgaaaac aaacagcgga
     4021 aagccgggtt cttcggccag catttaagaa aatacataca atccgatacc gccgccgtgc
     4081 gctatttgaa ccaatatgcc gcgaagcaaa gctttaagct ggctacttcg gtgtctacag
     4141 tcgtgctttt ttcgatcttt ggcatttcca atcttgcgca aaaaaatact ccggaaaatc
     4201 ctgacccgcc gacagtgaat atgggaagga agacaccggg tttgcaggtt ttgatctcgg
     4261 agtgcaaaat gctgggccgt ccaggcagat cgctatggga gtaaagttca atttttgacc
     4321 ggatcagcca tttcaaatcc tctgcccaag cctaaaaagt gaagtaaaac tgcagtgtac
     4381 gtgccagcgg acggccgcgg cagcgccggc cctggcaacc catcccgcta acagacagcc
     4441 gatcccgaca accgcccgcc catccccgcc aggagcctat ttctgtcgcc ccggagccaa
     4501 atttggtcca aatgttgtac cttgtaattt ccctcctctt aaaagcaata ccactgtccc
     4561 cgaagcctcc gatcataggc cctatggtaa ttttattgct ttttccaatt tctgacaaat
     4621 gaagcaccca agcattacct caaccgatcg ctttttgctg atctccttta tcctgtgtat
     4681 aacaggctcc ctgctgatct atcagcagat aaacaggcaa tacaccaggt ccaatttggt
     4741 gacccactct tatatcatca aggtcacttt aaatcaattc ctgctgaacc ttacggaagc
     4801 ggagactaat caacgcggct atctgttgtc gcatgattcc gatttcctga acgactatca
     4861 acgcgcgatc cggaaggcaa ggttcatcgt tggcatcctc gacagtctca ccatcgacga
     4921 tccgggacaa caggaaggcc tccgtaaatt acggcaggct ttcaacaata gcatgctcac
     4981 cctgcaggcc agtatgatgc tgccgacgga atcattctcc gacccccatt cggacgagat
     5041 catccagcat acgaaggacg tcctcgacaa tcttcgcggc aacgcgtata cgatgctcga
     5101 cgcggaagat agcctgctgc gaaaccgcgt cgtagcccga aacaacctgg caaagcgaaa
     5161 cccctggtat atgctcacca cgggtctgct ggttacctcg ctcatcttct ttgtctatct
     5221 caaactaaaa aaagaaaccc tcctccgtct ggactcggag cacaataccg ccgaactgga
     5281 gaaaaaggac ctgttgcgtt ccagggagct ggccagcctg aacgaacagc tgacgacaca
     5341 aaacaatgtt ttcctgcatg cggagaaggt cgccctgatc ggaagctata cctggaatct
     5401 ctccacgggc cagatgacat tctccgataa cctgttccgg ctactcggat acgatccggg
     5461 ggaattcact ccttcgcacg agcatctgct ccgcctgatc cacccggcgg aacgatcgga
     5521 gatcctgaaa aatactccct tcctgctgga gtcgatcatt caaaccgaga cgccttgccg
     5581 gtttatcacc aaagaaggca ccatccggta tttccgcgcc accggcaaac atctgtataa
     5641 acccgacggc gaggtcatcg ccggcacttt ccaggacatt accaaggaca tcgagctgag
     5701 acaggcactc gagaataacg acaatttcat ccgcagcctg ctggatatcg cccccagcac
     5761 gatcaccatt ttcgatctgg aaaaatccac tacggtattc tccagccatc cgcagctgcc
     5821 tactctgggg caccgcgtag acatctctgg tcccaacgat aacccgtatt cgctgatgca
     5881 tcccgacgac agggaacccg ccgcgcaata cctggagtcc cttcggcacc tgcaggaccg
     5941 tgaggtctcg gaattcgaat accgcatgaa gaacgacgag ggcggctata cttatttcat
     6001 gtcgcggaat acgccttaca aggcgaatag ccggcacgag atcatccaga tcctcggagc
     6061 taccatcgat atcaccgaga aagtaagagc gcagctggag gtgaaggcga aaacggaagc
     6121 cctactgagg tccagtttcg aacagcaaag cgcgatccgg ttcaagacgt tggcggattc
     6181 catgccccag atcgtctgga cggcggatgc aggcggaagg attgattatt tcaaccagcg
     6241 ctggtacagc tatctgcgtc tccccgacaa aacactgtcg acgagagata tcacggataa
     6301 tatgcatccg gaggacgtcg ccgactactt ccggaactgg aatatttctg tggaaaccgg
     6361 gaacccgttc caggtagaat tcaggttcag ggaccagaac aatcccgggc aatattgctg
     6421 gttcctggcg aaagccttgc ctatcaccga tgcgaacgca gtcattgtta aatggcatgg
     6481 gacgttcacg gatatcaatg atctaaagct ggcccagctg tcgatccagc agctggtaaa
     6541 ggagaaggag gattttatcc atatcgccag tcacgagctg aaaacgcccg ttaccagcct
     6601 caaaggttcg atccagctgc tcgaacggat gtcgggtgaa ggagccctgg cggaagaaat
     6661 ggacgtgctg atcgaaatgg ccgtcagaca ggtgaataag ctaagccgta tggttggcga
     6721 tctcctggac gtcgcccgta tggatgcgga caagatcgag ctggttggaa gagacttcga
     6781 gcttcaggag gcgatcgccg acagtctcga acaggtgcgg ttcctcgcgc cgggatctac
     6841 catccggatc gacggtccca ccgatatccg cattcactcc aataaagaac gtatcgagca
     6901 ggtgatcaca aattttttat ccaacgccat caaatattcg cccgaaggtg cggaaatcgt
     6961 tttggagccc tcgctgctgg acggtcaact gcggatctcg gtgacggatc aggggatcgg
     7021 cattccgtct tcgaagctgc cttttgtttt cgatcgattt ttccgggtgg aagggtcgtc
     7081 cgataagttc tcggggctgg gcctgggatt gtatatctct gcagggatcg tgtcgagact
     7141 ccggggggag atcggcgccg tcagcgagct cggacacggg tccaccttct ggttcacttt
     7201 gccggtgaat tcgcatgcgc agaacggcga gccacaggat atcattcact ccgcaaagcc
     7261 ttcaccggat tggctatggc agcacgcgcc gcctgccagc tgatcgtcgc caatgcgata
     7321 accacggcca gcagacccgc gacgacgaac acccaccatc ctatatcgat acgataggca
     7381 aaatcctgta accaccgatg catgatatac caggcgagcg gagtggcgat gaggatcgat
     7441 acgatcacca gtcgtataaa atctgccgaa agaagaacga tgatatgcgc taccgatgca
     7501 cccagcacct ttcgaacgcc gatctccttt gtccgctgca tcgtcgcgaa aagggatagt
     7561 ccgagcaatc ccagacaggc tataaatatc gcgaagcctg cgaatatgct gaaagccttt
     7621 ccgaacaacc ggtcactggc atattgctga ttgtaatggt catccaggaa atagtaatcg
     7681 aaatagttgc cggggaaaaa ggcgtcatag gtctttttta tagcgccgat ggtggccggc
     7741 atgtcactgg tattgacctt caccgagatg atgctgttgt tgctgtacgc cggcatgagc
     7801 atggtgggct cgacagggta gcgcagcgat ttctgatgat aatcgttgat cacgccgatg
     7861 acatcccatg tcttgtcgta gttcttgatc tgtttg
//
LOCUS       DASYSV010000121         4517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_537083, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000121 DASYSV010000000
VERSION     DASYSV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537083"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..524)
                     /locus_tag="VGQ51_03925"
     CDS             complement(75..524)
                     /locus_tag="VGQ51_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEV9035736.1"
                     /translation="MQKDQNKLLIMREDHNLISRYMNAFRKIKCSDAKTIQLLWERLR
                     NAIIIDNNLFPRDVARLNSTLIVKNLETNLAVAYTIVLPRDVDRRKNKVSILSPIGIA
                     LIGLKKGLCFNWERLARKKNFAVLEIYNSPSLERSHRKAYQSKFLSY"
     gene            complement(1265..1648)
                     /locus_tag="VGQ51_03930"
     CDS             complement(1265..1648)
                     /locus_tag="VGQ51_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9035737.1"
                     /translation="MTILLVDDDEDDRSLFIEAAKEVDSTIICHSASDGMEALAYLRD
                     DRNDVPDFIFLDLRMPGLSGEECLIEIKREPRMADVPVIVYTTSRDVRESVKLKQLGA
                     AHFASKPVSQEEVYFMLSFVIGERW"
     gene            1805..2335
                     /locus_tag="VGQ51_03935"
     CDS             1805..2335
                     /locus_tag="VGQ51_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV9035738.1"
                     /translation="MDSLLFVRNELDRLSLHGVSIGISEISTDEELSPSQYGTLREVL
                     KQAGIELFEDKKEILVQRIKNVIMEVVYHGNEPLVYNLSTYLSEKLQLDYTYMSNLFS
                     DSQNITIEKFYISHKIELVKELLLYEGMTLTEIGHKMHYSSLAHLSNQFKKVTGLTTS
                     QFKQQYARHDLPPETC"
     gene            2635..>4517
                     /locus_tag="VGQ51_03940"
     CDS             2635..>4517
                     /locus_tag="VGQ51_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEV9035739.1"
                     /translation="MAKSREKHKSGYSVSDGFPVVGVGASAGGLEAFKRLLKAIPETS
                     WMAFILVQHLEPNHESMLTEILQKSTPIPVEEITDNVHVEPNHIYIIPSNKLLTAYDG
                     RLRLQPRPKNKKSLPIDVFFASLAEVHGSHAIGVVLSGTASDGTQGLKAIKERGGLTF
                     AQEQGSAAFRGMPQNAIDAGVVDFVLTPEEIPLQIAKSTGVSKEAPPETAEDHPDPEG
                     AFRQLLTLLRLRKGTDFTYYKQKTIRRRIERRMGLNNIGTITAYLDFFKENPAEQDLL
                     YQDILIPVTQFFRDPLTYDAIRESLLPLLFAGKEEGSSLRFWIAGCSTGEETYSMAIC
                     LNEYLGARAAAFQIQIFSTDISEKSIAIARKGIYSRTEVGGLSPERLARYFEKVAGSY
                     HVNKSIREMCVFAHHDFLKMPPFARMDLISCRNVLIYMEPFLQKKVLATFHYALKENG
                     YLLLGHSETTAPAMDLFLPFDRKEKIYTRKPGPAKFLPASAGRTENATVGTEKPIRKE
                     SGRNDFQKSADILLLSHFSPPGVIVNDQLDIVEFRGSTGGWLQPGRGKPSLNILKLTK
                     DGLAFELRNALHKSRTSGQPQISENIPLAASGKQRWMTLEVHPLPRTAEPHFLILFRD
                     GTSG"
BASE COUNT         1163 a         1194 c         1091 g         1069 t
ORIGIN      
        1 cataacgctg aaggagtttg tagtcgtccc tcagcaacac aacctcgttg atagccgtat
       61 gcatatgcgt tgaattagta ggataaaaat tttgactgat acgccttcct atggctcctc
      121 tccagggaag gtgaattata gatctccaat accgcaaagt tctttttgcg ggccaacctt
      181 tcccagttaa aacataaccc ctttttcagc ccgataagtg cgatgccgat gggggacaat
      241 atcgatacct tattctttct tctatcgacg tcccgcggca gtacgatcgt gtaggccacg
      301 gcgaggttgg tttcaaggtt ctttacaatt agcgtcgagt ttagtcttgc cacatcccgg
      361 ggaaaaagat tgttgtcaat aataattgcg ttacgaagac gctcccaaag aagctggatc
      421 gtcttggcat cactgcactt gatcttcctg aaggcattca tatacctgga tatcaaatta
      481 tgatcttccc gcatgatcaa tagcttattc tggtcctttt gcataattat atgattttgc
      541 catagccgga atcaacgtcc tgtgcatctt tgacaaactt ccgccaaaca ggtaccgatg
      601 cgcgatcggg cattgacatc cggacacgtc ggcccgtgtg ttttaaaatt gaaattgctc
      661 gccaaaaaat gtcggaaggt ccccctggct gtcagaaact cagcaagggg acgatggtac
      721 taaagtcctt aaagttgggg aagaattggc gcctgtggca gaagtcaccg gtctgtggga
      781 aaaatgcatt tcgggcaata cgcaaacata ccagggatac agaagcaatg ctgccggtat
      841 ctattgtggt atgcttgtta ttattcatgt cccgtaaaat tacaattatt gttcgtacaa
      901 tatcgactga agcgcatatt gcattgttaa tgatttggca aaaaaagtga aggtcgtcca
      961 agcgaggcat gcatcatttt tctaccagct gcccgggtac atcctcaagg aacggatagt
     1021 tgaacctaag cccggtcaat cactaattca ttgccaaccg tccatacgcc tatcgccttc
     1081 caggctattc gctccgcttc atctttctgg tccgtggaat atacccggcc ggttagcgtt
     1141 acggttgact tcgatacctt gaccttgata ctacagccgg ttaacgataa cgaattcttc
     1201 aggatggctt caagatctga tttttcgatc atgctataag atttacgtcg aagattaatc
     1261 aattttacca acgttctccg atcacgaaag atagcataaa atagacctct tcctgggaaa
     1321 cgggcttgct tgcaaaatgt gccgcgccta gctgttttag tttcaccgat tcccgcacgt
     1381 cccgggaagt ggtatataca attaccggaa cgtcggccat ccggggctcc cgtttgatct
     1441 ctataagaca ctcttcgccc gagagcccgg gcatccgcag gtcgagaaag atgaaatccg
     1501 gcacgtcgtt acggtcgtct ctcagataag ccagcgcttc catgccatcg gaagcggagt
     1561 ggcagatgat cgtgctgtcc acctctttcg cggcttcgat aaagagactc cggtcatctt
     1621 catcatcgtc aaccaataat atggtcatat ggtagagttt ttcgcttcct gcatgtaagg
     1681 tagactaaag gacccgaatt tcatcccgga acagggtctg ccaaaaatta tgtaacttac
     1741 atgtcaggga gcagtctcaa ggcaaacatg tattttgaaa ctcttgataa agaatataat
     1801 tagtttggat tcactattgt tcgtccgcaa cgaactggat aggcttagcc ttcatggtgt
     1861 ttccatcggg atcagcgaga taagtacgga tgaagaactc tccccttccc aatatggtac
     1921 gctgcgcgag gtactgaagc aggccgggat cgaattattc gaagataaaa aggagatcct
     1981 ggtgcaaagg ataaagaacg ttattatgga ggtcgtctac catggcaacg aaccgttggt
     2041 atacaatctg tcgacctacc tgtccgaaaa gctgcagctt gactatacct atatgtccaa
     2101 cctgttctcg gattcgcaga atatcacgat cgaaaagttc tatatcagcc acaagatcga
     2161 actggttaag gaattgctgc tctatgaggg tatgacgttg acggaaattg gacataaaat
     2221 gcattacagc agcctcgcgc atctttccaa tcaattcaaa aaagttaccg gcctgaccac
     2281 ttctcaattc aagcaacaat atgccaggca cgatcttcca ccggaaactt gctaaacttt
     2341 tcttttagcc caaacctgtg aattttgtaa cctttccaat aaccttgtaa ttttccgggc
     2401 cggcgccgat ccggagacca tcgaccagct catcgttgcg tgtagttggc gccgagaccc
     2461 tgagccgcgt gatcgccccc cacgaccggt tccggcgaca cgggtatcct tggctccata
     2521 agccagacac atgcgctggt cgaaagctcc tggcatcctc ggatccggaa agatgtgatc
     2581 ccacgctaca agttcccata taaagcgtaa ctttccaaaa atcctgctgg cgtcatggcg
     2641 aaatcgagag aaaagcataa gtcaggatat tctgtatctg atggctttcc cgtagtcggg
     2701 gtcggcgctt cggccggcgg attggaagca ttcaaaaggc tgctaaaggc tattcccgaa
     2761 acctcctgga tggcgtttat tttggtgcag cacctggagc cgaaccacga gagcatgctc
     2821 acggagatcc tgcaaaaaag cactcccatc ccggtggaag agatcaccga caatgttcac
     2881 gtcgagccca atcatattta catcattccc tcgaacaagc tcctgacggc ctatgacggc
     2941 cgattacggc tccaaccgcg tccaaaaaat aagaaaagcc tgcccatcga cgtatttttt
     3001 gcttcgctgg cggaagtcca cgggtcccat gccattggcg tggtgctgtc cggaaccgct
     3061 tcggacggaa ctcagggact gaaagccatc aaggaacgcg gcgggctcac cttcgcacag
     3121 gagcaggggt cggcagcctt tcgcgggatg ccccaaaacg ccatcgatgc cggcgtagtg
     3181 gatttcgtcc ttacaccgga agagatcccg ctgcagatcg ctaaatccac aggggtatcc
     3241 aaagaagcgc cgccggagac agccgaagac cacccggacc cggagggcgc cttcagacaa
     3301 ctgctcacgc tgctacgatt gagaaaaggc accgatttca cctattacaa acaaaagacc
     3361 atccggaggc ggattgaacg ccggatggga ttgaacaata tcgggacgat aacggcttac
     3421 ctggattttt ttaaagagaa ccccgccgaa caggacctcc tctaccagga tatcctgata
     3481 cccgtcacgc aatttttccg cgaccccttg acctatgacg ccattcgcga atcgctgctg
     3541 ccgctgctat tcgccggtaa agaagaggga agctcgctgc gcttctggat cgcagggtgc
     3601 tccaccggcg aggagacgta ctccatggcg atctgcctga acgaatacct gggcgccagg
     3661 gccgccgcct tccagataca gatcttttca acagatattt ccgaaaaatc catcgccata
     3721 gcccgaaagg ggatctattc ccggacggaa gtgggcggcc tctcgccgga acggctagcg
     3781 agatattttg aaaaggtcgc cggaagctat cacgtcaata aatccattcg ggaaatgtgc
     3841 gtattcgctc atcacgattt cctgaaaatg ccgcccttcg cgcggatgga cctcatcagc
     3901 tgccggaacg tgctcatcta tatggaaccc tttttgcaaa aaaaagtgct ggcgacattt
     3961 cattatgcct taaaggagaa tggctatctc ttgttgggcc actcggaaac gaccgctcct
     4021 gccatggacc tgttcctgcc tttcgacaga aaggaaaaga tatacacccg caagcccggt
     4081 ccggcaaaat tcttaccggc atccgccggc cgcaccgaga acgcaacggt cgggacggag
     4141 aaacccatcc gaaaggaatc tggccggaac gattttcaaa aaagcgccga tatccttctg
     4201 ctgtcgcact tttcgccacc cggcgtcatc gtgaatgacc agctcgatat cgtggaattc
     4261 cggggctcca ccggcggctg gctgcaaccc gggcgcggta agccgagcct gaatatcctg
     4321 aagctgacaa aggacggcct ggcatttgag ctcaggaacg ccctgcacaa gtccagaacg
     4381 tcaggacagc cgcagatcag cgaaaacatc ccgttggcgg cgtccggaaa acagcggtgg
     4441 atgacgctcg aagtacatcc gttgccgcgc acagcggagc ctcatttcct gatccttttc
     4501 agggacggga cttccgg
//
LOCUS       DASYSV010000122         5616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1558244, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000122 DASYSV010000000
VERSION     DASYSV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558244"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VGQ51_03945"
     CDS             <1..1018
                     /locus_tag="VGQ51_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9035740.1"
                     /translation="RADTGVARNDVVMITCENTLKQYQAFKREIATLPWVQQTATANY
                     GFYKGLPFASVKTQTPGHNIGMGYMIVDTGFISLMGLKWKEKPASMITVMDGRHPILN
                     EVAVAAIGFSGRATGQIVTDQYQVGGVLKDFNYFSLARQIQPLELYIGLDTPAFGLQR
                     NILFVRYRPHSDISAMLKNIGQVYSSFDKISSFSYEFLDDAYDSQYKTEDRLARLMGV
                     FSTITVIIACLGLFALATFSAQQRVKEIGVRKVLGASVKRIASGLCIDFLKPVALSLL
                     LAVPISAWVMHGWLNNYAYRIPLNWQTFAVASGFMAILALVAVFFTSVKAATANPVTS
                     LRSE"
     gene            1192..1431
                     /locus_tag="VGQ51_03950"
     CDS             1192..1431
                     /locus_tag="VGQ51_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEV9035741.1"
                     /translation="MSFAEHIQRMQRIDGFVRRKATGNTLEFARKLGISRITVIRTIN
                     EMKDMGFPIKFCRTRCSYYYEYPGKMVEKMFIGMD"
     gene            1535..1720
                     /locus_tag="VGQ51_03955"
     CDS             1535..1720
                     /locus_tag="VGQ51_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035742.1"
                     /translation="MKISIQTVVDHKTTIPMLSGFSSHQNVQMVIPGACTTCNGSSCC
                     SCCGGVELSGSVTVAQA"
     gene            1772..2317
                     /locus_tag="VGQ51_03960"
     CDS             1772..2317
                     /locus_tag="VGQ51_03960"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9035743.1"
                     /translation="MSVGKIFEDYAQLISISLGVQEIARTAAFYEKTLGVKLAYSLSE
                     HRESLHSVISSGVQFAIDKAQRPEERGLMLHFAVGDLEKFKKNATGAGGKVVADNIPL
                     TIAEKVYSEFAAQHKGLKTTNKMGTLAIIKDPEGHSFAAIQLEEWARYLFAPGNITMV
                     ENDNQHEGIKLGERLKTLLKK"
     gene            2352..3311
                     /locus_tag="VGQ51_03965"
     CDS             2352..3311
                     /locus_tag="VGQ51_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEV9035744.1"
                     /translation="MKDPENIPSTPNEAFLNSIYSNTYYQVPLWYDSIGFRNNTLKYY
                     SAGEAGDAGTAEDFLVNSKYFGQNKEFQFSVGVFFEDAHMMMVHNRSDPWGNNRHFDY
                     SGFEKISLGKGIRLPVSFGDVIKGRRSKREFTGDPMSMDFLASILRCASGVSSIGSAS
                     LSDGSQVKIGFRTVASGGGLYPTDIIVAALNIQGLPGGIYQYVPQMDESGDFLIRLDE
                     NTDPVVRALPHSESSMNIDNAAAVFLLATNPWKSMRKYGSRGLRFIFLEAGGISQNIH
                     LASTALGIGSCDFGAFIDADIDRSLGFDGISQTVIHTIITGII"
     gene            3318..4349
                     /locus_tag="VGQ51_03970"
     CDS             3318..4349
                     /locus_tag="VGQ51_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HEV9035745.1"
                     /translation="MEEQTPRIKRYVTVIGHSPDTVELRVGVWNPISHILNDDESGGT
                     LYKILSLLKGDRTLPEIAQKAGIPLSKIEDIVSQLAQLNVIEYSPESGIDYYLNHAIP
                     ALQPRTDTIKKIKIIGSQTLGNIIYGLLEQCNYFDSIDILSTTGYEQLQNSSRAQSWE
                     KDSLAFEQLVSQYESWKGHYIVVASNQVDPLVMQAVNRICFALDLPFFYTTIDGPFLF
                     IGPTIVPGKSACWECLETRVTMNLRNSASYLNYKNALTNGRVHSIDYYPERIIQHLLS
                     THASMEILNYCLTGSSFTVNKVLSIYLPTMEIVFHEIFKVPSCKACYPSRTSRIRESY
                     YNLSTLLNT"
     gene            4346..>5616
                     /locus_tag="VGQ51_03975"
     CDS             4346..>5616
                     /locus_tag="VGQ51_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEV9035746.1"
                     /translation="MKISYSIRENNVSSTFPLAGRCFSPLTGLGVQIGSFLQGRSDSP
                     LICLGAQLTGVHYLLDRPEPKSSYHVGGSGLLEFEAFIKCIAETGERYSQLVSGIENE
                     HLIGFTTYDELKASHGDELLDLSCFDFFTPAQLSQPGFPFRSFGSASKYGFIRLESLI
                     GGWHRWVPVQLVLLGYNVRDRKGEQRILSAVTTGTATHTHRVKCLKNAILELIQIDSA
                     MGNWYGHSRSTRIVLDERTKNLSSILSKTLSDKSYEAGFYLLPNPDLAGFTIACLIDN
                     GHRIPSMVVGLGCCTSLESAMYKAFIEAVSCHQLARVISFKELFPENGIKREARIDTS
                     NIADLDTNVFYYSLPQNRISLLERFDTGVTMSASEAPRDMEGSEQHQTKMLIDSFRTS
                     GKELLFLDLTAHEVSELGMVTYRIWSPDTLSL"
BASE COUNT         1449 a         1467 c         1358 g         1342 t
ORIGIN      
        1 gcgggccgac acgggtgtag cccgaaatga tgtcgtcatg atcacctgcg aaaacacact
       61 gaagcaatac caggcattca aacgggaaat cgccacactg ccctgggtgc agcagaccgc
      121 aaccgccaat tatggctttt ataaagggct gcccttcgcc tccgtcaaaa cacaaacgcc
      181 gggacataat ataggtatgg ggtatatgat cgtcgacacc ggttttatct ccttaatggg
      241 cttgaaatgg aaggaaaaac cggcctctat gatcaccgtc atggacggcc gtcaccccat
      301 acttaatgaa gtggcagtag ccgcgatcgg gttttccggc agggctaccg gacaaatagt
      361 caccgaccaa taccaggtcg gcggtgtatt gaaggacttt aattacttct cgctcgccag
      421 gcagatccag ccgctcgaat tgtatatcgg cctggacacc cctgccttcg gcctgcaaag
      481 aaatatcctg tttgtccggt accggccgca tagcgacatc tcggccatgc tgaagaacat
      541 cgggcaggtc tatagctctt ttgacaaaat atccagcttc tcgtatgaat ttttggatga
      601 cgcttacgat agtcagtata aaactgagga ccggcttgcc cgcctgatgg gcgtcttcag
      661 caccatcacc gtcatcatcg catgtctcgg cttgtttgcc ctggcaacct tctccgcaca
      721 gcaacgggta aaggagatcg gcgtccgcaa agtgctgggg gcgtcggtga aacgtatcgc
      781 ctcagggctc tgcatcgatt ttctaaaacc cgtagctttg tccctgcttc tcgccgttcc
      841 catatccgcc tgggtaatgc acggctggtt gaataattat gcctatcgca ttcctctaaa
      901 ctggcagaca ttcgccgtag cttccggatt tatggcaata ttagcgttgg tcgcagtctt
      961 ttttacctcg gtaaaggcgg ccacggccaa cccggtaacc agtctccgtt cggagtgaaa
     1021 ccgacctcgt ctcgtagcat atctcatccg ctgccaaccg caatttcagg tgtttatact
     1081 cttgtttggc cctcgcgccc atcgtaactt gccctcgccg ctactaccac ctaagtcata
     1141 gtctttacta cccacgaaat actgaaaagt atacccgcat tattttgcag aatgtcattc
     1201 gcagaacata tccaacggat gcagcgcatc gacggctttg tcagaagaaa agcgactggc
     1261 aacactttgg agttcgcgag gaagctcggc atttcgcgga tcacagtgat ccgtaccatc
     1321 aatgaaatga aggacatggg gttcccgata aaattctgcc gcacgaggtg cagctattat
     1381 tatgaatatc cgggcaaaat ggtagagaag atgttcatcg gcatggatta aaaaacccgc
     1441 cccggtatca aaaaatgaga tcggtgtttt ggagctttaa cagtcgcaac cataattttt
     1501 aaaccattca acattaacct ttcaacatta acttatgaaa atatctattc aaacggtcgt
     1561 cgaccacaaa acgaccatcc ccatgctatc gggcttttcg tctcatcaaa atgtccagat
     1621 ggtcattccc ggcgcctgca ccacctgtaa cgggtcctcc tgctgctcct gctgcggagg
     1681 agtagaattg tcaggcagcg tgacagtcgc ccaggcgtga cagttccaaa attgatcgaa
     1741 aacattttta caactttcaa aactcaaagt catgagtgta ggaaaaatat tcgaagacta
     1801 tgcccaattg ataagcattt cactgggtgt gcaggaaatc gcaaggacgg ccgcctttta
     1861 tgaaaaaaca ttgggagtaa aactggccta cagtctctcc gagcacaggg agtccctgca
     1921 ttcggtgatt tcctcaggtg tacaattcgc gattgacaag gcgcaaagac cggaggagcg
     1981 tggtctcatg ttgcattttg cggtgggaga tctggaaaaa ttcaaaaaaa atgcgacagg
     2041 cgcgggggga aaggtggtag ccgacaatat cccgcttacc atcgcggaaa aggtatattc
     2101 cgaattcgcg gcgcagcata aaggtctgaa gactaccaac aagatgggaa cccttgctat
     2161 tatcaaggac cccgaaggcc attccttcgc ggccatccag ttggaagaat gggcacgtta
     2221 tctttttgcg ccggggaata tcaccatggt agagaacgat aatcaacatg aagggataaa
     2281 gctgggcgag cggctaaaga ccctgttaaa aaaataacgc cctctattcc caaccagcca
     2341 tctatcaggt tatgaaagat ccggaaaata tcccctcaac tcccaatgaa gcttttctca
     2401 attcgatcta ttccaatact tattaccagg ttcctctgtg gtacgactcc atcggcttca
     2461 ggaacaatac cctgaagtat tactctgcag gcgaggcggg cgatgccggt accgcagagg
     2521 atttcctggt caattcgaag tatttcggtc aaaacaagga attccagttc tcggtcgggg
     2581 ttttctttga agacgctcac atgatgatgg tgcataatcg ttccgacccc tgggggaaca
     2641 accgtcattt cgactactct ggtttcgaaa agatcagtct agggaaaggt atcaggttgc
     2701 cggtctcgtt cggagatgtg ataaaaggca gaagaagcaa acgggaattt accggcgacc
     2761 cgatgagcat ggattttctg gcatccattc ttcgttgcgc gtcgggcgtc agcagcatag
     2821 gcagcgccag tctttcggac ggttcgcagg taaagatcgg cttccggacc gtcgccagtg
     2881 gcggagggct ttatcctacc gacatcattg ttgcggccct gaatatacag ggacttccgg
     2941 gtggcattta tcaatatgtc ccgcagatgg acgaatcagg tgattttttg atccggctgg
     3001 atgaaaatac cgatccggtg gtccgggcat tgccacatag cgagtcatct atgaatatcg
     3061 ataatgcggc ggcggtattc ctgctcgcga cgaatccctg gaaaagcatg cgaaaatatg
     3121 gttcgagagg ccttcggttc atctttctcg aagccggagg gataagtcag aacatacatc
     3181 tggcatctac ggcactggga atagggtcct gcgatttcgg agcctttatc gatgcagata
     3241 tcgaccggtc gctgggattc gatggaataa gccaaacggt cattcatact attatcacag
     3301 gtataattta aatcccgatg gaagaacaaa ccccgagaat taaaagatat gtcacggtga
     3361 tcggtcattc tcccgacacc gtcgagctga gggtcggcgt ctggaacccg atctctcata
     3421 tcctgaacga tgatgagtcc ggcggcacac tatataagat cctttcctta ttgaaaggcg
     3481 accggaccct ccccgagatc gctcaaaagg ccggtattcc cctttcgaaa atagaggaca
     3541 tcgtcagcca gctggcacag ttgaatgtaa tagaatattc cccggagtca ggtatagact
     3601 attatttgaa ccatgcgatc cccgcgctcc agccaaggac ggacaccatc aaaaaaataa
     3661 agatcatcgg aagccagacc cttgggaaca tcatttacgg actcctcgaa cagtgcaatt
     3721 atttcgacag catcgatatc ttatcgacca ccggctatga acagttgcaa aacagctcga
     3781 gggcccaatc ctgggaaaag gactccctcg ccttcgagca gctggtcagt cagtatgaat
     3841 cctggaaagg acactatatc gtcgtcgctt ccaaccaggt agatccactg gtgatgcagg
     3901 ccgtcaatcg catctgcttc gcgctcgatc ttcccttttt ttatacgact atcgacggcc
     3961 cctttctttt tataggtccg acgatcgtac ccggaaaatc cgcctgctgg gaatgcctcg
     4021 aaacaagagt gaccatgaat ctccggaatt cggcatccta tctcaactat aagaatgccc
     4081 tcaccaacgg cagggttcat tccatcgatt attacccgga gagaataata caacacctgc
     4141 tctccacgca tgcttcgatg gaaatcctga attattgcct gacgggcagc tcattcaccg
     4201 tcaacaaggt cctgtcgatc tatctgccta cgatggagat cgtctttcac gagattttca
     4261 aagtcccctc ctgcaaggca tgctatcctt ccaggacttc caggatcagg gaatcctatt
     4321 acaatctgtc cacactcttg aacacatgaa gatcagttac tccatacgtg agaataatgt
     4381 atcgagcacc tttccgctgg ccgggagatg ctttagcccc ctgacgggcc tgggagttca
     4441 gatcgggtca ttcctccagg ggcgatcgga ctcaccgctg atctgtctgg gcgcacagct
     4501 gacgggtgtc cattatttac tcgacagacc cgaacccaaa agctcctacc atgtcggcgg
     4561 gagcggttta ctggaattcg aggcattcat aaaatgcatc gcggagacgg gggaaagata
     4621 ctcccaattg gtttccggca tcgagaacga acatctcatc gggtttacga cctatgacga
     4681 gcttaaagcc agccatggtg acgagctatt ggacctgtcc tgcttcgact tctttactcc
     4741 cgcccagctg agccaacccg gattcccttt cagatcgttt ggatccgcct cgaaatatgg
     4801 gttcatccgc ctggagtcac tgatcggtgg ctggcataga tgggtacccg ttcaattggt
     4861 ccttctggga tataacgtgc gggacagaaa aggcgaacag cggatactct ccgccgtcac
     4921 caccggtacc gccacacaca cccaccgggt gaaatgcctg aagaacgcga tactggagct
     4981 gatacagata gattccgcta tggggaattg gtatggtcat agccggtcca cgaggatcgt
     5041 tttggatgag cgaacgaaaa atctgtcttc cattctttca aaaacccttt ccgataaatc
     5101 ctatgaggcc ggcttttacc tgttgccgaa cccggatctg gcagggttta caattgcctg
     5161 tctcatcgac aacggacacc gcataccttc gatggtcgtc gggctgggat gttgtacttc
     5221 cctcgaatca gccatgtaca aagcatttat cgaggcggtg agctgtcatc aattggccag
     5281 ggtcatctca ttcaaagagc tattccccga aaatggaata aagcgggaag caaggatcga
     5341 tacatccaat atcgctgacc tggataccaa cgtattctat tattccctgc ctcaaaacag
     5401 gatatccttg ctggaacggt tcgataccgg cgtaacaatg tccgcctccg aggcgccccg
     5461 ggatatggaa ggctccgagc agcatcagac aaaaatgctg atcgactctt tcagaaccag
     5521 cggcaaagag cttctctttc tcgatctcac tgcgcatgaa gtaagcgagc tcggaatggt
     5581 cacttacagg atatggagtc ccgatacgct gagcct
//
LOCUS       DASYSV010000123         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1558256, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000123 DASYSV010000000
VERSION     DASYSV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558256"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VGQ51_03980"
     CDS             <1..275
                     /locus_tag="VGQ51_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9035747.1"
                     /translation="ESLGHLFSLERGALRGALVHSAILDWEDAPSIRGGYSFETVGGE
                     TERAALAGPIEDTLFFAGEAVYNGPVPGTVEAAFSSGITVAELIAG"
     gene            complement(354..806)
                     /locus_tag="VGQ51_03985"
     CDS             complement(354..806)
                     /locus_tag="VGQ51_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEV9035748.1"
                     /translation="MQDVKETIEVLNDLVQINNDRIVAYEKAIRQTKTEDTDMKILYA
                     SMVAEGHRIKIALATEVQALGAEIEQGTTTSGKIYRGWMDVKAIFTGHNRHTILANCL
                     SGEEAIQKAYRMALGHDLPAHIRELLLRQRDALLASLEEIASLRDQYA"
     gene            complement(914..1243)
                     /locus_tag="VGQ51_03990"
     CDS             complement(914..1243)
                     /locus_tag="VGQ51_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEV9035749.1"
                     /translation="MSTEELISAREFCTVHHIELSFIRHLHDAGLIGMTVMDGGFFLA
                     TGELPAVEKFVRWHYDLAINSEGIEALSHVLERMERLLAENRALRNRLHRYEQGPHGS
                     VTPAEEL"
     gene            complement(1253..2185)
                     /locus_tag="VGQ51_03995"
     CDS             complement(1253..2185)
                     /locus_tag="VGQ51_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEV9035750.1"
                     /translation="MDFIDYYKILGIGKDATEEDIKKAYRKLARKHHPDLNPNDKEAN
                     KKFQQINEANEVLSDPEKRKKYDQYGKDWMHAEAFEKAQQSGNRGGQGGARGFEGFGE
                     GFGGGFTGDFNTGEGGFSDFFESLFGRSSGRGSGGGARGQFRGQDYQAELEISLADAA
                     TTHQRVLTVGGKNIRITIPAGVENGQVIRLKGHGAPGSNGGPDGDLYLTFSIADDPRW
                     TREGNDLHTKIPLDLYTAVLGGEITVDTLTGKVKLKIKPGTQNELRLKGKGFPVYKQD
                     GQYGDLYVTPEIRLPTDLTEKEKELFRQLAALKH"
     gene            2272..2520
                     /locus_tag="VGQ51_04000"
     CDS             2272..2520
                     /locus_tag="VGQ51_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035751.1"
                     /translation="MQRTRNILFAGILLVTGTLGLARCKAPGQNPDNTATDTSPMVSA
                     PDNNSANNPSLADTAYEKNRTPPITDTLKPRDTSRHRR"
     gene            complement(2560..>2956)
                     /locus_tag="VGQ51_04005"
     CDS             complement(2560..>2956)
                     /locus_tag="VGQ51_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9035752.1"
                     /translation="SIRESGARIHSGELPLLRAYPTELKLLFQNLITNAVKFRQPDRY
                     PEISISATSVDGQWKFIIGDNGIGIEKQFLERIFIIFQRLHNRTEYEGSGIGLAHCKK
                     IVELHGGTIWADSQPGNGSRFHFTISKDI"
BASE COUNT          602 a          805 c          811 g          738 t
ORIGIN      
        1 ttgaaagcct tggacatctg ttctcgctgg agcggggggc gcttcgtgga gcgctggtcc
       61 attcggcaat cctggattgg gaggatgccc cctctatccg gggaggctat agcttcgaga
      121 cggtgggcgg cgaaacagaa cgcgctgcac ttgcggggcc gatcgaggat acgctctttt
      181 ttgccgggga ggctgtatat aatgggcccg tccccgggac ggtcgaggcg gctttcagca
      241 gtggtatcac cgtggcggag ctgatcgccg gttgagtgta tcgatcgcga aatggggtga
      301 tctcgtcgat acttaagtgt atcgatcgcg aaatggggtg atctcgtcga tacttaagcg
      361 tattgatccc gcagggaggc aatctcttcg agagacgcga gaagcgcatc ccgctgacgg
      421 agcagcagct ccctgatatg agcgggaaga tcatgcccca atgccatgcg gtaagctttc
      481 tggatggcct cttctcccga caggcagttg gcgaggatgg tgtgccggtt gtgtcccgta
      541 aaaatcgcct ttacgtccat ccacccccga taaatcttgc ctgaggtggt cgttccctgc
      601 tcgatctccg cgccgagggc ctggacctcg gttgcaaggg cgatctttat ccgatgtccc
      661 tccgctacca ttgaagcata caatatcttc atgtcggtgt cttccgtctt tgtttgccgg
      721 atagctttct cgtaggcaac gatccggtca ttgtttatct gcacgagatc gttcagcact
      781 tcaatcgttt ccttgacatc ttgcataatg cttgtttaat aagatatcag cgtcaaaatc
      841 atgccaaaat ggaaagaaag gcttcagaac cgggcgggcg ggaaaaatct tcctcagcct
      901 acagctcttc ggcctacagt tcctcggccg gtgtgaccga tccatggggg ccctgctcat
      961 aacggtgcag acgattcctg agagccctgt tctcggcgag caggcgctcc atacgctcca
     1021 gcacatgcga caaggcttcg ataccttcgg aattgatggc gaggtcgtaa tgccagcgga
     1081 cgaatttttc cactgcgggc aattcgccgg tggcgaggaa gaaaccaccg tccatgaccg
     1141 tcattccgat cagacccgca tcgtggagat gccggatgaa ggacagctcg atatgatgaa
     1201 cggtgcaaaa ttcgcgggcg gaaataagtt cttcggttga catacacgat gtttaatgtt
     1261 tgagtgcggc caattggcgg aacaattctt tttccttttc ggtcagatcg gtgggcaacc
     1321 ggatctccgg ggtcacatag agatcgccgt attgtccatc ctgtttgtaa accgggaatc
     1381 cttttccctt gagacgcagc tcgttttgcg tccccggttt gatcttgagc tttacttttc
     1441 ccgtaagggt atcgacggtg atctcgccgc cgagcacggc cgtatatagg tccagcggga
     1501 tcttcgtatg cagatcgttg ccttccctcg tccatcgggg atcatcggcg atcgaaaagg
     1561 taagatacag atcaccgtca ggtccgccat tggagccggg cgccccgtga ccttttagcc
     1621 tgatcacctg tccattttcc acaccggcag gaatggtgat ccggatattc ttaccgccaa
     1681 cggtgagtac acgctgatgg gtcgtcgcgg catcggccag gctgatctcc agctctgcct
     1741 gatagtcctg accgcggaat tgaccgcgtg cgccgccacc gctaccccta ccggaggagc
     1801 gaccgaacaa cgattcgaaa aagtcggaga atccgccttc tcccgtattg aagtcgccgg
     1861 tgaagccgcc tccgaaacct tccccaaaac cttcgaagcc cctggcaccg ccttgtccgc
     1921 cgcggttgcc cgactgctga gccttttcga acgcttccgc atgcatccag tctttaccgt
     1981 actgatcgta cttctttctt ttttcgggat cgcttaacac ctcattggcc tcgtttatct
     2041 gctggaattt tttgttcgct tccttgtcgt tggggttgag atcggggtga tgtttcctgg
     2101 cgagtttgcg gtaggccttc ttgatatctt cctccgtcgc atccttgcct attcccagaa
     2161 ttttataata atctatgaaa tccattgcaa cgcctttttt tacaaagtta ggtcctgtag
     2221 ggaaacgaaa tgcgctggca tagttttttt agcgctgctt aaaagaaggt tatgcaaaga
     2281 acgcgcaaca tccttttcgc ggggatatta ttggtgacgg ggacgcttgg tctggccaga
     2341 tgcaaggctc ccggccagaa tccggataat accgccaccg atacgtcacc catggtgtcg
     2401 gcgccggata acaacagcgc caacaatcct tcgctggccg atacagccta tgagaagaac
     2461 aggacgcccc cgatcaccga tacgttgaaa ccgcgtgata cttcgcgtca tcggcgctga
     2521 ccggccgccg cgtcttctgc aggcgcctgc cggtgctgac tatatatcct tactgatcgt
     2581 aaagtggaac cggctgccat ttccgggctg tgagtccgcc cagatggtac ctccatgcag
     2641 ctccacgatc tttttgcaat gtgcgagccc gataccggaa ccttcgtatt ccgtgcggtt
     2701 gtgcagccgc tggaagatta taaagatacg ttccaggaat tgtttttcga taccgatgcc
     2761 gttgtcgccg atgatgaatt tccattgtcc gtcgacggat gtcgcagata tggagatctc
     2821 cggatagcga tcgggctgcc ggaacttgac ggcgttggtg atcaggttct gaaagagcag
     2881 cttgagctcg gtaggatagg cccggagcaa agggagttca cctgaatgga tacgcgcccc
     2941 gctttcccgg atactt
//
LOCUS       DASYSV010000124        11256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_921502, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000124 DASYSV010000000
VERSION     DASYSV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11256
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921502"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGQ51_04010"
     CDS             <1..415
                     /locus_tag="VGQ51_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9035753.1"
                     /translation="GRLQHIRDLSRGDYNTYIGYDEGLSHLLYAGADFILMPSRVEPC
                     GLNQLYALRYGTVPVVRNTGGLHDTVIDYGDRGGYGIRFLHASVQDMTHGIWRSVELF
                     QQPGMLKEIRQHMMQLDYSWESSVSTYIALYESMR"
     gene            443..1717
                     /locus_tag="VGQ51_04015"
     CDS             443..1717
                     /locus_tag="VGQ51_04015"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV9035754.1"
                     /translation="MSKEVLSVILGGGAGTRLYPLTASRSKPAVPIAGKYRLVDIPIS
                     NCLNSGISRMFVLTQFNSASLNRHIKNTYHFSAFSSAFVDILAAEQTPDNPTWYQGTA
                     DAVRQCLRHLGPFDSQYVLILSGDQLYQMDFMEMLENHKKAGADISIATIPVNDRDAS
                     DFGIMKMDGNGFITSFTEKPKKDMLPPWVSDTGDAMRAVGRNYLASMGIYIFNRQLLS
                     DLLQNEFAESTDFGKEIIPQSLEKYKVVSYQYDGYWTDIGNIPSFFEANLGLTKDIPD
                     FNLFDNEKSIYTRARMLPPAKISGTTLEKTIIAEGCIINASRIENSVIGIRTRIGTGT
                     TIVSSYLMGVDYYETIEEIELARAHGLPTVGIGHRCYIRNAIVDKNCRIGDDVRINGG
                     HHLENSDHSLYTVKEGVVVIKKGAVLPNGFVI"
     gene            1730..3157
                     /locus_tag="VGQ51_04020"
     CDS             1730..3157
                     /locus_tag="VGQ51_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9035755.1"
                     /translation="MANQPSSVGGGTTGVQKPLLTNRQIISMSFGFLGIQCGFALQNA
                     NTSRILQSFGADVDQLPAFWLVAPIMGMIVQPLIGHYSDRTWNRLGRRKPYFLTGALL
                     SALALILLPNSGSMAGIVPALWIGAGFVMVMDASFNVAMEPFRALVADNLPDRQRAKG
                     FAIQACLIGIGAVVGSYMTSILTWLGFSQVSGQNGVADNIKYSYYIGSTIFIVAILVT
                     VLFSKEYPPDEYARYHGHADTTGNKAGLGEIIRDFRKMPRTMRQLGLVQFFSWFAMFS
                     MWVFAFPAVATHVYGLAGGYEHTKAGNDAGNIVGNAFGIYNGVGAIYALCLTPMARRL
                     GRKMTHAISLFAGGIGLISIFFVHGQWGLYGSMIGVGLAWASILAMPYVILSGCIPAG
                     KMGIYMGIFNFFITLPQIVNGLFGGWIEKHIYGGQPIYSIVLAGFCLIAAAVSVLFVY
                     DGGAIRMQQEKAGFYAERVASSSNP"
     gene            3160..5001
                     /locus_tag="VGQ51_04025"
     CDS             3160..5001
                     /locus_tag="VGQ51_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV9035756.1"
                     /translation="MQRLSVFFAACLLCLTATAQLSTKVYPTNWWVGMKLSTIQVMIR
                     GEGVGEAKTVTVRYPGVSVVDWHHTANPHYLFLHLKIGPDTKPGTVKIILQNDREIDF
                     PLYARRSGNGTEYAQGVTSKDFIYLLMPDRFSNGDPSNDRVPGMRDQSLDRDSIFLRH
                     GGDFQGILNHLDYLQSLGVTALWMTPVIENDMPNRTEHGYAFTDHYSIEPRLGGAAMY
                     RRLSDSLHRRGMKLIQDAVYNHVGLYNWFVQDLPEKSWLHQWPHYTQSNFRDQPLFDP
                     HGSAIDKKRESDGWFTHEMPDLNQDNPDVAEFLIEHAIWSVETFGVDGWRIDTYIYCS
                     LPFMNKCNSALIAEYPHITMFGETWVHGTANEAYFVRNNLNVPFKSNLMGAVDFQLLF
                     SGIQPAIKDTTGSWDTGIGSLYNTLSNDFLYQDPTRNVLLLDNHDMTRWFSQNDEDVA
                     AQKMGIEWLLTERGIPQLYYGTEICMKGIDNPDGWVRLDFPGGWAGDRKNAFTGEGLS
                     DDEKAIRHLVRTLGQFRLHSTALRTGKMMQYIPEKTLYVYFRYDDRQTVLCAMNTGNR
                     PVTLDFSRFSERTHGFSQGTDVLTGERHPLAQPAQLPGRTMWVLELQ"
     gene            complement(5024..5779)
                     /locus_tag="VGQ51_04030"
     CDS             complement(5024..5779)
                     /locus_tag="VGQ51_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HEV9035757.1"
                     /translation="MKKPGWPSFIALLLLAVARPAAAQNAIFLSSGKIEFERKVNVFA
                     QIGNADDQWTELQRKMSNHFKTSYFDLVFTPEKSLYKPGRESGDKDNFFFVQTPAQDN
                     TVWSDLAHDRSVSTKNVFEQTFLVQDSLRHIRWKITDETRTIAGFNCRRANAIIMDSI
                     YVVAFYTDEIITTGGPESFSGLPGMILGVSLPHQHVSWFATKVEAIPISESQVAAPVK
                     GKKVDNTALKETVRGSLKDWGKTGQQYMEAILL"
     gene            complement(5790..8588)
                     /locus_tag="VGQ51_04035"
     CDS             complement(5790..8588)
                     /locus_tag="VGQ51_04035"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV9035758.1"
                     /translation="MPKPTFLLSCCLLLSVSLLAQSSHITGSVADTLEKKPLVNASIL
                     LLRPADSILVRHTRSDAAGHFKLQGVPAGHYLLLVTYPKYADYVDEVEVKDSGELGLP
                     PVPMVLKSTLLESVVVKGVGAVRIKGDTTEFNADSFRTEAGANVEDLLRKLPGIQVDR
                     DGKITAQGESVKKILVDGEEFFGDDPTLVTQNLRADMIDKVQVFDKKSDQAVFTGIDD
                     GQREKTINLKLKDSKKNGYFGRATASAGTDGYYDEELMANYFRRKEKLAVYGIISNTG
                     KTGLNWNERDNYGQSILGMADVDASTGAISIEYNGDDLDSWSGQYQGQGFPAVKTGGL
                     HYNNKWNDDNQSINGNYKYMDLSVTGNSNTNSQYILPDTVYYNSQRQLFNNQITRQTL
                     DGTYETKFDSTTSLKIMANGGTDHKTTFSGYNSVYLASDSSLVNSNDRTTTTTGDNRV
                     LNGNLLFRKRFAKAGRTFSLNARENYTDNTSSGYLNSETKLYSGGSPYSDSVIDQFKD
                     YHTTKLLIDSRAVYTEPLSKTSFLMANYGVEIDNNRSNRDSYNKALGGKYSLLDSLYS
                     NDYQYNVLTQRTGLAYRYTGKKLKVSAGSDIGISRFDQRDLHADTVSTRRFVNWFPTA
                     TVSYAFSAMRRLSLQYNGSTSQPSVTQIQPIVTNEDPLNIYIGNPALKPKFDNRISLY
                     FDDYKVLTDRGIWANIWSDFTSNAISSSVNVDSAGRSVHQYVNVKGIWTMNGYLQYSL
                     KWKKPDIRFNIHINTNQSNNSSIVNGLTNDTRSGTMSMGTGIYKSKEKKYLVGIDLSA
                     SYTSSSSSINTGIVTQYWTWQIQPNADLFLPLKFQIHADADINLRQKTPTFQTNNNVV
                     LINAWLGKKLLARDALLIKVSVNDLLDQNIGFNRTVNSNFITQNTYSTIRRFAMLSVV
                     WNFTKAGTTAPKSSIEIN"
     gene            8676..10628
                     /gene="glgB"
                     /locus_tag="VGQ51_04040"
     CDS             8676..10628
                     /gene="glgB"
                     /locus_tag="VGQ51_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV9035759.1"
                     /translation="METSTHQRYEEVHFVDSTKPVWNYSLFTDEDIRNFQAGTHYRLY
                     QLFGSHRVTVLGTEGYYFAVWAPNATAISVIGNFNNWQPDQHPLFVRLERSGIWEGFI
                     PGVPKGEAYKYHIRGYNGGTLDKGDPYSWFWEMRPMTASITWELDYDWKDTDWMQKRA
                     ARNSLNAPWSIYEVHLASWMRPDPFDEERYNTYDQIRELLVPYVKQMGFTHVELMPVM
                     EHPFDGSWGYQGTGYYAPTSRFGDPQGFMALVEAFHRENIGVILDWVPSHFPYDYHGL
                     YLFDGANTYEYADMRKGFHPDWNSYVFNYRRGEVKSFLISNARYWLDKYHTDGIRVDA
                     VSSMLKLNYSRQEGAWEPNEYGGDGNLEAIAFIKDLNATIFRDFPDVQTIAEEATDWP
                     GVSRPTFAGGLGFGMKWMMGWMHDTLDFFKMDPVFRRDHLDKFTFSLMYYYDENFMLP
                     LSHDEVVHGKSPMIYKMPGDDWQRFANLRLLYTYMFTHPGGKLLFMGNEFGATTEWNY
                     KTELPWQLLQHDSHRLLRDCVRDLNGLLRNEPAMYENQFTVYGFEWVDLRHRDESVAV
                     YRRKGRDPQGDVLVILNFTPVVRRNWKVYTHGKSAWKEIFNSDDRKYWGTGDTNNPSI
                     PVTPTKDEGWFELNVHLPPLGAVVLK"
     gene            complement(10634..>11256)
                     /gene="argS"
                     /locus_tag="VGQ51_04045"
     CDS             complement(10634..>11256)
                     /gene="argS"
                     /locus_tag="VGQ51_04045"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV9035760.1"
                     /translation="VELPTGRMKTREGTVVDADDLLDEMIAEATRKTKELGKTEDFSA
                     DELEVLYATIGLGGLKFFLLRVDPKKRMVFNPEESIDLHGYTATFIQYAYARIRSILR
                     KAGTPAAATASSNDNLLPLEKALTVVLEKFPTVLEQACAEMNPSVVASYAFELAKTFN
                     SFYAEHSVAGAETPEKQALRLQLCRLTATVLRNAMNVLGIRVPDRM"
BASE COUNT         2475 a         2979 c         3123 g         2679 t
ORIGIN      
        1 aggaaggctg cagcacatac gggacctgtc caggggcgac tacaatactt atatcggcta
       61 tgatgaaggg ctcagtcacc tgctgtacgc gggggcggac ttcattctga tgccctcccg
      121 tgtagagcct tgcggtctta atcagcttta tgccctacgg tatggcacag ttcctgtagt
      181 tcgcaatacg ggaggtcttc acgatacggt cattgattat ggggacagag gaggttatgg
      241 catccggttc ctccacgcgt cggtccagga catgacccat ggcatctggc gaagtgtcga
      301 gctgttccag cagcccggga tgctgaagga gatcagacaa cacatgatgc agctggatta
      361 cagctgggag tcgagtgtca gcacttatat cgctctttat gaaagcatgc gctagaggag
      421 tcttacttta aatgtttcct atatgtcaaa agaagttttg tccgtaatat tgggaggggg
      481 agccggaaca cgcctgtatc ctctcacagc aagccgctcg aaaccggccg ttccgatcgc
      541 agggaagtac cggctggtag acatccctat ctccaactgt ctgaattcgg gtatctcacg
      601 aatgttcgta ctgacccagt tcaattcggc gtccctgaac cgccatatca agaataccta
      661 tcatttcagc gccttcagct cggccttcgt ggacatcctc gcggcggagc aaacgccgga
      721 taacccgacc tggtaccagg gtaccgccga cgccgtccgg caatgtctcc ggcatcttgg
      781 gcccttcgac agccaatacg ttctcatact gtcgggtgat cagttgtacc agatggattt
      841 catggagatg ctggaaaacc ataaaaaggc cggtgcggat atctctattg ccaccatccc
      901 ggtgaatgac cgggatgcat ccgatttcgg gatcatgaaa atggatggga acggctttat
      961 cacctccttt acggaaaaac ccaagaagga tatgcttccg ccctgggtaa gcgatacggg
     1021 cgatgccatg cgcgccgtcg gccgcaacta cctggcgtcg atgggcatct atatcttcaa
     1081 ccgccagctg ctgtccgatc tgcttcagaa tgaatttgcc gaatccacgg atttcggaaa
     1141 agagatcatc ccgcaatcgc tcgaaaagta caaggtcgtc agctatcaat atgatggtta
     1201 ctggaccgat atcgggaata tcccctcatt tttcgaggcc aatctgggtc tgaccaagga
     1261 tattccggat ttcaatctct tcgacaacga aaaatccatc tatacccgcg cgcgcatgct
     1321 gccgcccgcg aagatcagcg ggaccaccct cgaaaagacc atcatcgccg aaggatgtat
     1381 catcaatgcc tctcgtatcg agaacagcgt gatcggtatc cggacccgca tcgggacggg
     1441 taccaccatc gtcagcagct atctgatggg ggtcgattac tatgaaacga tcgaagagat
     1501 agagctcgcc cgtgcgcacg gtctgccgac ggtaggcatc ggccatcgct gttatatccg
     1561 caacgccatc gtggataaga actgccgtat cggggacgac gttcggatca acggcggtca
     1621 tcatctcgaa aattccgatc attcgctgta cacggtgaag gaaggagtgg tggtgatcaa
     1681 gaagggcgcg gtgcttccca atgggttcgt gatctaacat catttcaata tggccaacca
     1741 accatcctcc gtcggcggtg ggacgacagg cgttcaaaag cccctgctga ccaacaggca
     1801 gatcatttcc atgagctttg gcttcctggg gatccagtgc ggttttgccc tgcagaacgc
     1861 caataccagc cgcatcctcc aatccttcgg ggccgatgtc gatcaactgc ccgccttttg
     1921 gttggtggcg ccgatcatgg gaatgatcgt tcaaccgctg atcggccatt atagcgaccg
     1981 cacctggaac cggctgggac gccgtaaacc ttatttcctt accggcgcgc ttctctccgc
     2041 gctggccctg atcctgcttc ccaactccgg cagcatggcg ggaattgtgc cggctctttg
     2101 gatcggcgcg ggcttcgtga tggtcatgga cgcttcgttc aatgtggcga tggagccttt
     2161 ccgggcgctg gtagcggaca atctgccgga tcgccaacgg gcaaagggtt ttgcgatcca
     2221 ggcctgtctt atcggtatcg gcgcggtggt cggctcctat atgaccagca tcctcacctg
     2281 gctcggtttc agccaggtgt cgggccaaaa tggcgtcgcg gacaatatca aatattccta
     2341 ttacatcggt tccaccattt ttatcgtcgc tattctcgtg accgtccttt tttccaaaga
     2401 atatcctccg gacgaatatg cacggtatca tgggcacgcg gatacgaccg ggaataaggc
     2461 cggtcttgga gagatcatcc gggattttcg caaaatgccc cggaccatgc gtcaattggg
     2521 gctcgtacaa ttcttcagct ggtttgcgat gttcagcatg tgggtattcg ccttccctgc
     2581 cgtggctacc catgtctacg gtctggctgg gggctatgaa catacaaagg cggggaatga
     2641 tgcgggcaat atcgtcggca acgccttcgg catctacaat ggcgtgggcg ccatatacgc
     2701 gttgtgtctg acgccgatgg cgagaagact tggtcgtaag atgacccatg ccatttcgct
     2761 ttttgccggc gggatcggcc ttatctcgat cttttttgtc catgggcaat gggggctcta
     2821 tggctccatg atcggggtag gtctggcctg ggccagcatc ctcgccatgc cttatgtcat
     2881 cctcagcggc tgtatccccg ccggaaagat ggggatctat atgggcatct tcaatttctt
     2941 tatcacgctt ccacagattg tcaatggtct tttcggggga tggatcgaaa aacatatcta
     3001 tggcggccaa ccgatctatt ccatcgtttt ggccggcttc tgtctcatcg ccgcggccgt
     3061 ctccgtcctg ttcgtttatg acggcggtgc gatacgtatg caacaggaaa aagccggttt
     3121 ctatgctgaa agggtagcct cttcctcaaa tccatgatta tgcaacgact ctccgtattc
     3181 ttcgctgcct gcttgctctg cctgacggcg actgctcagc tttcgacaaa ggtatatccg
     3241 accaactggt gggtcggaat gaaactttcc acgatccagg tcatgattcg gggcgaaggg
     3301 gtgggggagg cgaagaccgt caccgtccgc tatcccggcg tgagcgtcgt cgattggcat
     3361 catacggcca acccgcatta tctttttctc catctgaaga tcggccccga caccaaaccg
     3421 gggacggtaa agatcatcct gcaaaacgac cgggagatcg acttcccgct gtacgcccgc
     3481 cggtcgggta acggcaccga atacgcgcag ggtgtcacgt caaaagactt catttacctc
     3541 cttatgcccg accggttcag caacggcgac ccctccaacg accgcgtccc gggcatgcgc
     3601 gaccagtcac tcgatcgcga ctctatcttt ttaagacacg gcggcgattt ccagggtatt
     3661 ctcaaccacc tcgattatct ccagtccctc ggcgtcaccg cactttggat gacgccggtc
     3721 atcgagaacg acatgcccaa tcgcaccgag cacgggtatg cctttaccga ccactatagc
     3781 atcgaacccc ggcttggcgg cgcggcgatg tatcgaaggc tctccgactc gttgcacagg
     3841 aggggtatga agctgatcca ggatgccgtc tataaccacg tcggtctgta taactggttc
     3901 gtccaggacc tgcccgagaa atcctggctt caccaatggc cgcattacac ccagtccaat
     3961 ttccgggacc agccattgtt cgacccacat ggaagcgcga tcgacaaaaa gcgcgagagc
     4021 gacggctggt tcacacacga gatgccggac ctcaaccagg acaatcccga tgtcgcggaa
     4081 ttcctgatcg aacacgccat ctggtccgtc gagaccttcg gcgtcgatgg ctggcgtatc
     4141 gacacctata tctattgcag tctgccgttc atgaacaagt gcaattcggc actgatcgcc
     4201 gagtacccgc atatcaccat gttcggagag acctgggtac atggcacggc caacgaagct
     4261 tattttgtgc gcaacaacct gaatgtcccg ttcaaaagca acctcatggg cgccgtcgat
     4321 tttcaattgt tgttctccgg tattcagcct gcaataaaag acaccaccgg cagctgggat
     4381 acgggaatcg gatcgctcta caataccctc agcaacgact ttctttacca ggaccctacc
     4441 cgtaatgtcc tcctgctcga caaccatgac atgacccgct ggttctcgca gaacgacgaa
     4501 gacgtagccg cccagaagat ggggatcgaa tggctgctga cggagcgcgg catccctcaa
     4561 ctgtattatg gcaccgagat ttgcatgaaa gggatcgaca accccgacgg ttgggtccgt
     4621 ctcgatttcc cgggcggctg ggcgggggac aggaagaacg cgttcacagg agaagggctc
     4681 tcggatgacg agaaagcgat ccggcatctc gtccggaccc tgggacagtt ccggctgcat
     4741 tcgacggcgt tacgcactgg taaaatgatg cagtatatcc cggaaaaaac gctgtatgtc
     4801 tatttccggt acgatgaccg gcagaccgtg ctttgcgcaa tgaatacggg caaccggccg
     4861 gtaaccctcg atttcagccg cttttccgaa cggacgcacg gcttttcaca ggggaccgac
     4921 gttctcacgg gtgaacgaca tcccttggct caacccgcac agctacccgg ccggacgatg
     4981 tgggtgctgg aactacaata gacgaaccgt ccgcgcgcag atcctacagt aagatcgctt
     5041 ccatgtattg ctgaccggtc ttaccccaat ctttcagact gccgcgaacc gtctctttca
     5101 atgcggtatt atcgaccttt ttccctttta ccggggcggc gacctgcgat tcggagatcg
     5161 ggatggcctc tacctttgtc gcaaaccaac tgacatgctg gtggggcagc gagacgccga
     5221 ggatcatgcc cggaagaccg ctgaaagact ccggcccgcc ggtcgttatg atctcgtcgg
     5281 tatagaaggc gaccacatag atagaatcca tgatgatggc attggcgcgc cggcaattga
     5341 aaccggcgat cgtccgcgtt tcatcggtga tcttccaccg gatatgccgg agactatcct
     5401 gtacaagaaa ggtctgctcg aagacatttt ttgtactgac ggacctgtca tgagcgagat
     5461 cggaccacac ggtattgtcc tgcgcagggg tttgaacgaa gaaaaagtta tccttatcac
     5521 ccgattccct cccgggtttg tagaggcttt tttcaggcgt gaaaacgaga tcgaaatagg
     5581 aagtcttgaa atggttggac attttcctct gcagctccgt ccattggtcg tccgcattgc
     5641 caatctgtgc gaagacattg accttccgct cgaactcgat cttgcccgag gacaggaaaa
     5701 tggcattctg cgcagccgcc ggccgggcga cggcgagaag aagcagggca atgaatgagg
     5761 gccatccggg ctttttcatt ttgtcttatt taatttattt cgatcgagga ttttggcgcc
     5821 gtcgttcccg ccttcgtgaa attccatacg accgacagca tggcgaaccg cctgatcgtg
     5881 ctataggtgt tctgggtgat aaaattactg ttgacggtac ggttgaatcc gatgttctga
     5941 tcgagcagat cgttgacgga cactttaatg agcagggcat cgcgcgcaag cagcttctta
     6001 ccgagccacg cattgatgag caccacattg ttattggtct ggaaggtcgg cgttttctga
     6061 cgcagattga tatcggcatc ggcatggatc tggaatttca gcggaagaaa gagatcggcg
     6121 ttgggctgga tctgccaggt ccagtattgc gtgacgatgc cggtatttat gctcgagctg
     6181 ctgctggtat agctggcgct gagatcgatg cctacaagat atttcttttc ctttgattta
     6241 tagatgcccg tacccatcga catcgtcccg ctgcgggtgt cgttcgtcag accgttgacg
     6301 atactggaat tgttgctctg gttggtgttg atatggatgt tgaaccggat atcgggtttt
     6361 ttccatttga gcgaatactg cagatatcca ttcatggtcc agatgccctt tacattgacg
     6421 tattgatgga cgctgcggcc ggcggaatcg acattgacgc tgctactgat ggcattggaa
     6481 gtaaagtcgg accagatatt ggcccagatg ccgcggtcgg tcaaaacctt gtaatcgtcg
     6541 aagtacaggc tgatccgatt gtcaaacttg ggtttcagcg ccgggttgcc gatatagatg
     6601 ttcaaagggt cttcattggt gacgatgggt tgtatctgcg tgaccgaagg ctgtgaggtg
     6661 ctgccgttgt attgcaggct gagccgccgc atggcgctaa aggcgtagga aacggtggca
     6721 gtgggaaacc agttgacgaa ccggcgggtg gataccgtat cggcatgcag atcgcgctgg
     6781 tcaaaacgcg aaataccgat atcggagccc gcagagacct tcagcttctt cccggtataa
     6841 cggtacgcca gccccgtccg ctgggtcagg acattgtact ggtagtcgtt actgtacagc
     6901 gagtccagca gggaatattt accgcccagc gccttgttgt aagaatcgcg gttggaacgg
     6961 ttgttgtcga tctccacacc gtaattggcc atcaggaaag aggtcttcga gagcggttcg
     7021 gtatagacag ccctggaatc gatcagcagc ttggtggtat gataatcctt gaactggtcg
     7081 atcacgctgt ccgagtaagg gctacctccg ctatagagtt tggtctcgga attgagatat
     7141 cccgacgacg tattgtctgt gtaattctcc cgcgcattga gcgagaaggt ccgcccggct
     7201 tttgcgaatc gcttgcggaa aagcaggtta ccgttgagga cacggttatc accggtggtc
     7261 gtggtggtcc ggtcgttgga attgaccagg ctgctgtcgc tggccagata gaccgagtta
     7321 tacccgctga aagtggtctt atgatccgtg cccccgttgg ccatgatctt gagcgacgtc
     7381 gtggaatcga attttgtctc gtaagtaccg tccaaggtct gccgggtgat ctgattgttg
     7441 aacaactggc gctggctgtt gtaatagacc gtatcgggca ggatatattg cgaattggta
     7501 ttgctgttgc ccgttacgct gagatccata tacttataat tcccgttgat ggattggttg
     7561 tcgtcgttcc atttgttgtt gtaatgcagt ccacccgttt ttaccgcggg gaagccttgt
     7621 ccctggtatt gtcccgacca gctgtcgaga tcgtcgccgt tatactcgat actgatcgca
     7681 cccgtagaag catccacatc cgccattccg agtatgctct gtccgtaatt gtccctttcg
     7741 ttccagttga gaccggtttt accggtattg gaaatgatgc cgtaaacagc gagcttttct
     7801 tttcggcgga aatagttagc catgagctct tcgtcataat atccgtcggt gccggcgctg
     7861 gcggtcgccc ggccgaaata gccgttcttc ttgctgtcct tcagcttcag attgatggtc
     7921 ttttcgcgct gaccgtcgtc gatgccggta aagacggcct gatcgctttt tttatcgaag
     7981 acctgcacct tgtcgatcat gtccgcccgg aggttctgtg tgacgagagt gggatcgtca
     8041 ccgaagaatt cctcgccatc gaccagtatt ttcttcaccg actcaccctg cgcggtgatc
     8101 ttgccgtcgc gatcgacctg tataccgggc agcttccgga gcaggtcttc gacgttggcg
     8161 cctgcttctg tgcgaaaact gtcggcattg aattcggttg tatccccctt gatgcgtaca
     8221 gctcccacgc ctttgaccac tacggattcc agcagcgtgc tcttcagcac catcgggacg
     8281 ggcggcagcc ctagttcgcc cgaatccttc acctccactt catccacata gtcggcatat
     8341 tttggatagg tgacgagcag gagatagtgt ccggcgggga cgccctgcag cttgaaatga
     8401 ccggccgcat cgctgcgggt atgacgaacg aggatcgaat cggcggggcg cagtaaaagg
     8461 atggaggcgt tgaccaacgg ttttttctcg agcgtatcgg cgacggaccc ggtgatatgc
     8521 gaagattgtg ccagaagcga cacggagaga agcagacagc aggataagag aaaggtcggt
     8581 ttaggcatgg atcggagcaa ttagcgaatt gaaattaggt gcaaatggat ggatatacaa
     8641 cgcctaaagc ttaaatttac gttaaagaga cgaaaatgga aacttcgaca catcagcgat
     8701 acgaagaggt gcatttcgtg gattcgacga aaccggtttg gaactactcc ttattcacgg
     8761 acgaggatat ccgaaatttc caggcaggta cgcattaccg cctctaccag ttgttcggca
     8821 gccatcgtgt gacggtgctc gggaccgaag gttattattt cgccgtatgg gcgccaaacg
     8881 ccaccgcgat atccgttatc ggcaacttca ataactggca gccggatcaa catccgctgt
     8941 tcgtgcggct cgaacgttcg ggcatctggg aaggtttcat accgggcgtg ccgaagggcg
     9001 aggcttataa atatcatatc cgcggataca acggcgggac gctggacaag ggcgatcctt
     9061 attcctggtt ctgggaaatg cggcccatga ctgcctccat tacctgggag ctggactatg
     9121 actggaaaga taccgactgg atgcaaaagc gtgcggcgcg caactcgctg aacgcgccct
     9181 ggagcatcta tgaagtgcat ttggccagct ggatgcggcc cgatcctttc gatgaggaac
     9241 gttacaatac ttacgatcag atccgcgagc tgctcgtacc ctacgtgaaa cagatgggct
     9301 ttacgcatgt ggagctgatg cccgtcatgg agcatccctt cgacggcagc tggggctacc
     9361 aggggacggg atactatgcg ccaacttcac gtttcggcga tccgcagggc ttcatggcgc
     9421 tggtagaagc ctttcaccgc gaaaatatcg gcgtgatcct ggattgggtg ccttctcatt
     9481 ttccttatga ctatcacggg ctttatcttt tcgacggggc taacacctac gaatatgcgg
     9541 atatgcggaa gggtttccat cccgactgga acagctatgt cttcaactac agaaggggag
     9601 aggtgaaatc cttcctgatc agcaatgccc ggtactggct cgataagtat cacactgacg
     9661 gaatcagagt ggacgccgtc agctcgatgc tgaagctgaa ttattcccgt caggaggggg
     9721 cgtgggaacc caatgaatac ggtggcgacg ggaatctcga agcgatcgcg ttcattaaag
     9781 atcttaacgc aacgatcttc cgcgacttcc ccgatgtcca gaccatcgcc gaggaggcga
     9841 ccgactggcc gggcgtgagc cgtccgacct ttgcgggggg actggggttc ggtatgaagt
     9901 ggatgatggg ctggatgcat gataccctgg atttttttaa gatggacccg gtcttccgcc
     9961 gtgatcacct ggataagttc acgttcagcc tgatgtatta ctatgacgaa aacttcatgc
    10021 tgccgctgag tcatgatgaa gtggtacacg gtaaatctcc gatgatctat aaaatgccgg
    10081 gcgacgactg gcagagattc gcgaatctca gactcctata tacctatatg ttcactcatc
    10141 cgggcgggaa gcttcttttc atgggaaatg aattcggtgc gactactgaa tggaattaca
    10201 agaccgaact gccctggcaa ctgctgcaac acgactcgca caggctgctg cgggattgcg
    10261 tacgcgatct gaatggattg ctgcggaatg aaccggcgat gtatgaaaat caattcaccg
    10321 tctatggttt cgagtgggtg gatcttcgtc accgggatga aagcgtggcc gtctacaggc
    10381 gtaaaggtcg cgacccgcag ggagatgtgc tggtgatcct gaattttacc ccggtggtgc
    10441 gccggaactg gaaggtgtat acgcatggga agtcggcctg gaaagaaata ttcaacagcg
    10501 acgaccgcaa atactggggc acgggggata ccaataatcc ttcgataccg gtgacgccga
    10561 cgaaagatga gggatggttt gagctcaacg tgcatctccc gccgttgggg gcagtggtgt
    10621 tgaaatgatc acttcacatt cggtccggta cgcgaatacc gagtacattc atggcattgc
    10681 gcaggacggt agccgtcagc cggcaaagct gcagacgcag cgcttgtttt tcgggggttt
    10741 ccgcgcccgc gacgctatgt tccgcataga aggagttgaa ggtttttgcc agttcgaacg
    10801 cataagaggc cacgaccgag gggttcattt cggcgcaggc ttgttccagc accgtcggga
    10861 atttctccag caccaccgtc aacgcttttt ccagtggtaa caggttgtca ttagatgatg
    10921 cggttgcagc cgcgggggtg cctgcctttc ggaggatgct ccggatacgc gcataagcgt
    10981 attgaataaa ggtggccgtg tatccatgca gatcgatcga ttcctccggg ttgaagacca
    11041 tccgtttctt cgggtccacg cgtaataaaa agaatttaag accgccaagg ccgatggtgg
    11101 catacagaac ttccaactca tcggccgaaa agtcttcggt cttgccgagc tcttttgtct
    11161 tacgggtcgc ctcggcgatc atttcatcca gcaggtcgtc ggcatcgacg accgtacctt
    11221 cacgcgtttt catgcggccc gtgggcagct cgacca
//
LOCUS       DASYSV010000125         7107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1946312, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000125 DASYSV010000000
VERSION     DASYSV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946312"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VGQ51_04050"
     CDS             complement(<1..917)
                     /locus_tag="VGQ51_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV9035761.1"
                     /translation="MEHQDELSVALLQMQDNLRKVADEHAESLWLQSAMNILNAKLRG
                     YRSLSDLSKNVLDTLASFCEAQIGAFYVLEEDAFQVQYRFGATGPLPPPFRVGEGLAG
                     QAAADRQLKVVHPVPDNYFQIESAIGRHSPAAIVLVPTLFDEKVVAVIELGKFGECSP
                     LHLRLLEQISEPVAIAVNALLAKKDLEGLVRQLNGKEKELNGRITAINRSNAAVEFDL
                     NGIILSINQQYLDMTGYSEEEVLGRHHSIFVEKGVEDTKEYRDFWKRLKKGEFIQGVF
                     KRATKSGEPIWLQGNYNPLIDASGKPDRVL"
     gene            1084..1785
                     /locus_tag="VGQ51_04055"
     CDS             1084..1785
                     /locus_tag="VGQ51_04055"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV9035762.1"
                     /translation="MRLNTSSGRPADISDDQRAVSAFAGSLLLYFVAKRHRIDSLLML
                     GGGYLLYRAVSGHCPVSQALKARPRADGSNINIRTSVVVNKPRADVYAFWRRLENWPL
                     FMRHLENVDELDGNRSVWRLNMPGMDEIRWDARVVKEEKDAELSWHSVPGAPIESTGK
                     INFSDTPGDATRIDVLLSYRAPSGALGEKLSRLLTPAFRSRVEEDIRNFKHYFENIGI
                     EDSRVDGKNAGIVRS"
     gene            1800..2555
                     /locus_tag="VGQ51_04060"
     CDS             1800..2555
                     /locus_tag="VGQ51_04060"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEV9035763.1"
                     /translation="MLKGKKQRKYLAKKGREWQDQLQLFGRDRKEEALHDLRVAVKKI
                     KAFTRLSKACSGRAAAKDFRLLKKMFRQAGAVRDLGNLLGELDHFHSVPQEFRQQKQL
                     LQQTETTVFIDNIGEYRKKGKKAGRRLLHDVHAIPAACIRNWYAEQLVDIGVLLTASG
                     DRLHKARKKIKELLYVLKVLPSGLVEGLALDAKYLNDLQDAIGKWHDTVMVVTSWAGT
                     DRDGGLEMVRECRERESAVRRLGDDFFLRAHVS"
     gene            complement(2561..3241)
                     /locus_tag="VGQ51_04065"
     CDS             complement(2561..3241)
                     /locus_tag="VGQ51_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV9035764.1"
                     /translation="MPDIPCKLDHCFLCRHCMPEWKDVTALHRKTLNFRKGKSLFGQG
                     EPVKGIFFIYSGAVKIHAPWGDGREMIIRWAKAGDIAGLRGLGGNAVFPISATALEDT
                     TACFIPNEFLELSLKANPSLTYTLMHFYATELQAAEKRMRDLTHMEVKGRIAGALLDI
                     AAIFGTNPEGFLGVTLTRQDIAAYAGTTYETAFKFFTELTAAEVISTSGKDIRINQPE
                     RLTRLAGR"
     gene            3333..5033
                     /locus_tag="VGQ51_04070"
     CDS             3333..5033
                     /locus_tag="VGQ51_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV9035765.1"
                     /translation="MRTKASSTIPLSTSLGNLDEHGVSKRTRPVNKRVFYLSIQVVCN
                     AIIIGVVAKGLVYLIDFITNVSFYGRFSFEATSPAGNQLGLAVIAIPIIGAIAVGIMA
                     RFGSRAIGGHGIPEAMEKIILEESRIPPAITFLKPLSAALSIGTGGPFGAEGPIIATG
                     AAFGSVTGQWIHISSSERKIVLAAGACAGMAAIFGSPLAAVLLAVELLLFEFSPRSVI
                     PVALACVTGAGMHYLLFSTRPVFLMPDIPSPNTDALLIYVGLGAFVGVIAAMVSRSVY
                     WIEDMFEKLPVHWMWWPAIGAVAIGVIGYFAPHTMGVGYDNIQLLLTGVAPLAIVGSL
                     CFLKYLSWSISLGSGTSGGTLAPLFTIGGALGCLLGAAILHYFPHSGINIATAALIGM
                     AAMFAGASRAWLTSIVFALETTGQIHGLLPLLGGCVAAYFVSFFLMNGSIMTEKIQRR
                     GVKTPDAYEPDILQGASVRQLVEPLESDQTPVEYIHASEDAGLAAEMMGRHNTGSLAV
                     RDDKDGDKVIGMITAHGLLKFYSDHRENERHYESPARTRRMLARGRKLLRNINHEKYE
                     "
     gene            5026..5454
                     /locus_tag="VGQ51_04075"
     CDS             5026..5454
                     /locus_tag="VGQ51_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035766.1"
                     /translation="MSKENYHLFHKAMMTGLFVGIIDTLICLAYNIGYRNYTGYIPSS
                     FINVSSLIFVVNLTMTIVGIVFYAFHRNLKQGDIVFFIFFLALTAFLAWKISGIHRFD
                     DVKDNSSFKGLLGGVVVVLGISGACLPFLFKSQKFVDAII"
     gene            complement(5545..>7107)
                     /gene="ligD"
                     /locus_tag="VGQ51_04080"
     CDS             complement(5545..>7107)
                     /gene="ligD"
                     /locus_tag="VGQ51_04080"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEV9035767.1"
                     /translation="QKHDASHLHYDFRLEMEGVLKSWAIPKGPSLNPADKRLAMMVED
                     HPFDYKDFEGIIPEGNYGAGTVMVWDEGTYEPLEPAGASKTVQNRALLKQLRDGSLKF
                     RLNGKKLKGEFALVHTKGRGDNAWLLIKHRDRYATDSPVTDKDKSVLSKKTIEQIAHN
                     PRAKQWQSNKQATAKTTTRPQRNAIPAPASRSTKAIARTSKSSPATTSPKDKLLNPDD
                     DTQTVRLNKHELSFTHLSKVFWPKEKYTKRDLLNYYYEMASYILPYMKDRPQSLNRHP
                     NGITGPGFYQKNVSGKVPEWIETFPYRTAEDDEDKEFLVCTDEASLLYIISLGCIEVN
                     PWSSRVGKPDNPDWCIIDLDPDKKTPFDKVIEAAQVTKKVLDAAGVESWCKTSGSTGL
                     HVYFPLGAKYSYEHSKEFARLVVQLVQGQIPDYTTTERSVKARKGKMYLDFLQNRPQA
                     TIAAPYSVRPKPGATVSAPLHWDEVKKGLRMQDFTSAPMPARVKEMGDLFQPVLGRGI
                     DMQKTLKTLESL"
BASE COUNT         1461 a         1926 c         1997 g         1723 t
ORIGIN      
        1 agcaccctgt ccggttttcc cgaggcgtct atcagcggat tgtaattccc ctgcagccag
       61 atcggctccc cgctctttgt ggcgcgtttg aacacgccct gtataaattc tccctttttc
      121 agccgcttcc agaaatcgcg atactccttt gtatcttcaa cccctttttc gacgaaaatg
      181 ctatggtgcc tgcctagcac ctcttcctcg ctgtaccccg tcatatcgag gtattgctga
      241 ttgatgctca ggatgatgcc gttgagatcg aattctaccg ccgcattgct tctgttgatg
      301 gccgtgatac ggccgttcag ctctttttcc tttccgttga gttgacgcac cagcccctcg
      361 agatcttttt tggcaagaag cgcattgacg gcgatggcca ccggttcgga tatctgttcg
      421 agcagccgca gatggagagg gctgcactcc ccgaattttc caagctctat caccgcgacg
      481 actttctcat cgaacagcgt gggtacgaga acgatagcag cagggctgtg gcggccgatc
      541 gccgattcta tctggaaata attatccggg accgggtgga ccaccttgag ttgtcggtcc
      601 gcggccgcct gaccggcaag tccttcccct acccgaaaag gcggcggcag gggcccggtc
      661 gccccaaaac gatattgaac ctggaatgcg tcctcctcga gcacatagaa ggcgcctatc
      721 tgcgcctcgc agaaagaggc cagtgtatcc aggacgtttt tggataggtc ggacaggctc
      781 ctgtatcccc ggagctttgc gttcagtatg ttcatagcgg actgcagcca gagactctcg
      841 gcatgttcat ccgcaacttt ccgcaggttg tcctgcattt gcagcagggc aacgctgagt
      901 tcatcttgat gttccatgaa tagttgggtt cgtttactca tttgcgacag gtagaggcca
      961 tggatatcgg ctttccaaat tacaatttcc gtacctgatc gcaaaaatct tcggcttcgg
     1021 gggccagggc gcgcagacgg ccgtcgtatt agcatattat ttgcagcgta tggaagaaag
     1081 actatgcgcc tcaatacctc ttccgggcga ccggccgata tcagtgacga tcagcgtgca
     1141 gtatcggcat tcgcaggttc acttttactc tattttgtag ctaaaagaca taggatcgat
     1201 tccctgctca tgttgggcgg cggatattta ctttaccgcg ccgtttcggg gcactgccct
     1261 gtttctcagg ccttaaaggc aaggcctcgt gccgatggtt cgaacatcaa tatccggacg
     1321 agcgtcgtcg tgaacaaacc gcgtgcggat gtctatgcct tttggcgaag actggaaaac
     1381 tggccattgt tcatgcggca cctcgaaaat gtggacgagc ttgatggcaa cagatcggtt
     1441 tggcggctta atatgcccgg catggatgag atccggtggg atgcgagggt cgtcaaggaa
     1501 gaaaaggatg cggagctatc ctggcatagc gtgccgggtg cgcctatcga gagtacggga
     1561 aagatcaatt tttcggacac gccgggcgat gccacgcgta tcgacgtcct tctttcatat
     1621 cgggctccgt cgggggcgtt gggcgagaaa ttgtcccgtc tgcttacccc ggctttccgc
     1681 agcagggtag aggaagatat ccgtaatttt aaacattatt tcgaaaatat cgggatcgaa
     1741 gattcgaggg tcgacggaaa gaatgccggg atcgtgagga gttgagcaaa aatctcccca
     1801 tgcttaaagg aaagaaacaa cggaagtacc ttgccaaaaa aggcagggaa tggcaggacc
     1861 agctgcagct cttcggccgc gaccggaaag aagaggcgct gcacgatctc cgcgtcgccg
     1921 tcaaaaagat caaggctttc acccgtcttt cgaaggcctg ttccgggcgg gcggcggcaa
     1981 aggatttccg cctgctgaaa aagatgttcc gtcaggccgg cgctgttcgc gatctcggta
     2041 acctgctcgg tgagctggat cattttcatt ccgttcccca ggagttcaga cagcaaaaac
     2101 aactgctgca acaaaccgaa accacggttt tcatcgacaa catcggagaa tatcgcaaaa
     2161 aagggaaaaa agcgggccgc aggctgttgc acgatgtgca cgctatccct gcggcttgca
     2221 tacggaactg gtatgcggaa cagctggtag atatcggggt gcttctgacg gcgtcgggcg
     2281 accggctcca caaggcgcga aaaaaaataa aggaattgtt atatgtgctg aaggtgctgc
     2341 catccgggtt ggtcgaaggg ctcgctttgg atgcgaaata tcttaacgat ctccaggacg
     2401 ccatcgggaa atggcatgat acggtgatgg tggtgacgtc atgggcaggt accgatcggg
     2461 atggtggcct ggaaatggtt agggaatgca gggaaaggga aagcgcggtg cgccggctgg
     2521 gggatgattt tttcctgcgg gcgcatgtgt cgtgagggag ctatcgtccg gccagacggg
     2581 tcaaccgttc aggttgattg atccggatgt cttttcccga agtcgaaatg acctcggcgg
     2641 ccgtcaactc tgtaaagaac ttaaaggcag tctcataggt ggtccccgca taggcggcaa
     2701 tgtcctgccg ggtgagggtc acccccagga aaccttcagg attcgttccg aagatagctg
     2761 caatgtccag cagcgcgccc gcgatccttc ctttgacctc catatgggtg agatcccgca
     2821 tccgcttctc cgccgcctgc agctccgtcg catagaaatg catcagcgta taggtcagag
     2881 aagggtttgc tttgagactg agctcgagga attcattggg gatgaaacag gcggtggtgt
     2941 cctccagtgc ggtggcggag atgggaaata ccgcattgcc gcccagaccg cgaagaccgg
     3001 cgatatcccc ggcctttgcc caacggatga tcatttcacg gccgtcgccc cagggcgcat
     3061 ggatcttgac cgccccggaa tagatgaaaa agatgccttt taccggctcg ccctgtccga
     3121 aaagggactt ccctttccgg aaattaaggg ttttccggtg cagcgcggtc acatccttcc
     3181 attccggcat gcagtggcgg cataaaaagc agtgatccag cttacaagga atgtcaggca
     3241 taatatggtt tgagcaatat aattaattaa aatttagcgc aattttcgtg aaaaaacatg
     3301 gtttatacaa tatctttgca tttcgaaaaa atatgagaac gaaagctagt agcaccatcc
     3361 ctctctcgac atcgctgggc aatcttgatg agcatggagt atcgaaacgg acccggccgg
     3421 tgaacaaacg ggttttttat ctcagcatcc aggtcgtttg taatgccatt attatcgggg
     3481 tagtcgccaa agggctcgtt tacctcatcg acttcattac gaacgtttct ttttacggcc
     3541 gattttcttt cgaggccacc tcccctgcgg gtaatcagct ggggcttgca gtcatcgcca
     3601 ttccgatcat cggcgccatc gcggtaggga ttatggcccg ttttggatca agggccatcg
     3661 gggggcatgg tatccccgaa gctatggaaa aaatcattct ggaagaaagc cgcatcccgc
     3721 cggcgataac ctttctcaaa cctttatcgg cggccctttc catcgggacg ggcggtccgt
     3781 tcggcgccga aggtccgatc atcgctaccg gcgccgcttt cggttcggtg acgggtcagt
     3841 ggatacatat ctcctcctcc gaacgtaaga tcgttctggc ggccggcgcc tgcgccggta
     3901 tggcagccat tttcggcagt cctctggcgg cagtcctgct ggcagttgag cttctccttt
     3961 tcgaattctc accgcgttcg gtaatccccg tcgcgctggc ctgtgtcacc ggcgcgggta
     4021 tgcattatct gttgttcagc acccgtccgg tcttcctgat gcctgacatc cccagcccca
     4081 atacggatgc gctcctgatc tatgtgggat tgggcgcctt tgtcggcgtg atcgccgcta
     4141 tggtaagccg gagcgtctat tggatcgagg acatgttcga gaaactaccc gtccattgga
     4201 tgtggtggcc ggccatcggc gccgtcgcca taggcgtcat cggctatttc gctccgcata
     4261 ctatgggtgt cggctatgac aatatccagc tgctgctcac cggagtcgcg cccctcgcca
     4321 tcgtaggatc gttatgcttt ctgaaatacc tgtcctggtc gatcagcctg ggcagcggta
     4381 catcgggcgg tacgctggcg cccctgttca ccatcggcgg cgcgctcggc tgtctgctcg
     4441 gtgcagccat tctgcactat ttcccgcaca gcgggatcaa tatcgctacc gctgcgctta
     4501 tcggtatggc agcgatgttt gcaggagcct cccgcgcctg gctgacctcc atcgtcttcg
     4561 cactcgagac gacgggccag atccatggcc tgttaccgtt gctcggcggg tgcgtagcgg
     4621 catatttcgt cagtttcttc ctgatgaatg gtagcatcat gaccgaaaag atacagcgtc
     4681 gcggcgtcaa gacaccggat gcctatgagc ccgacatcct tcagggcgca tccgtcagac
     4741 agctagtaga accactggag agcgatcaga cgcccgtgga atatatccat gcctccgaag
     4801 atgccggtct ggccgctgaa atgatgggcc ggcataacac cggtagcctc gccgtcaggg
     4861 acgacaaaga cggggacaag gtgatcggga tgatcacggc acacggtctc ctgaagttct
     4921 acagcgatca ccgcgagaac gagcggcatt atgagtcgcc cgcccgcacc cggcgcatgc
     4981 tggcacgcgg aagaaaatta ttgagaaaca taaatcacga aaaatatgag taaggaaaac
     5041 tatcacctct ttcacaaagc gatgatgaca gggctgttcg tcggtatcat cgacaccctc
     5101 atttgcctgg cctataacat cggctaccgg aactacaccg gttacatacc gagttcattc
     5161 attaacgtct cttcgctgat ctttgtcgtg aatctgacga tgaccatcgt cggtatcgtt
     5221 ttttatgcct tccaccggaa cctcaaacag ggagacatcg tcttcttcat ctttttcctg
     5281 gcgctgacgg ccttcctggc ctggaagatc tccggtatcc accgttttga cgatgtcaag
     5341 gacaattcca gcttcaaggg tcttttgggc ggggtcgtag tcgtcctggg tatcagcggc
     5401 gcctgcctgc ctttcctttt caaaagccag aagttcgtag acgcgatcat ctagggacgg
     5461 ctcttgcgcg gatgggttca tgccgggggg gcgcgatggc cggagccgcg ggcgaatggt
     5521 ccggagccta tgcatggagc gcctctacag cgactccagc gttttcagcg tcttctgcat
     5581 atcgatcccg cgtcccagca ccggctggaa aaggtcgccc atttctttta ccctcgcagg
     5641 catgggcgcg ctggtgaaat cctgcatcct cagtcctttc ttcacttcat cccagtgcag
     5701 cggcgcgctg acggtagcgc cgggtttggg tctcaccgaa tagggcgcgg caatcgtcgc
     5761 ctgcggcctg ttctgcagga aatcgagata catcttcccc ttacgcgcct tcacgcttcg
     5821 ttcggtagtt gtatagtccg gtatctgacc ctgtaccagc tgtacgacca gccgcgcgaa
     5881 ttccttggaa tgttcatagc tgtatttggc gcccagggga aagtatacat gcaatccggt
     5941 ggaacccgag gtcttgcacc aggattcgac acccgcggca tccagcactt ttttggtgac
     6001 ctgtgccgcc tcgatgacct tatcgaaagg cgtttttttg tcggggtcga gatcgatgat
     6061 acaccagtcg gggttatcgg gctttccgac acggctgctc cagggattca cctcgataca
     6121 acccaatgag atgatatata gcaggctcgc ctcgtcggta cagaccagga attccttatc
     6181 ctcgtcatct tcggctgtgc gatagggaaa ggtctcgatc cactccggaa ctttcccgct
     6241 gacattcttc tgatagaaac cgggaccggt gatcccgttg ggatgccggt tcagcgattg
     6301 cgggcggtcc ttcatatagg gcaggatata ggacgccatc tcataatagt aattgagcag
     6361 atcgcgtttc gtgtatttct cttttggcca gaagaccttg ctgagatgag tgaaagataa
     6421 ttcgtgtttg ttcagccgga ccgtttgcgt atcgtcgtcc gggttgagca atttgtcttt
     6481 cggggaggtc gttgccggtg aagacttcga ggtgcgggcg atggctttgg tcgaccggga
     6541 ggccggggcc ggaatggcgt tgcgttgcgg gcgggtcgtg gtcttcgctg tcgcctgctt
     6601 gttgctttgc cattgtttgg cccggggatt atgcgctatc tgttcgatgg tctttttcga
     6661 cagtaccgat ttatccttgt ccgttacggg agagtccgtc gcatagcggt cgcggtgttt
     6721 gatcagaagc caggcattgt cgccccttcc tttcgtatgc accagcgcga attcgccctt
     6781 cagcttttta ccgttgagcc ggaatttcag cgagccgtcg cgcagctgtt tcagcagggc
     6841 gcggttttgc accgtcttcg atgcgccggc cggttccagc ggttcatagg ttccttcatc
     6901 ccacaccatt accgtcccgg cgccatagtt gccctcgggg atgatgccct cgaagtcttt
     6961 gtaatcgaaa gggtgatctt cgaccatcat cgccagccgt ttgtccgccg gattcagcga
     7021 aggacccttg gggatcgccc agcttttcaa aacgccttcc atttccaggc ggaagtcata
     7081 gtgcagatgc gaggcgtcgt gtttttg
//
LOCUS       DASYSV010000126         3431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_663857, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000126 DASYSV010000000
VERSION     DASYSV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663857"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VGQ51_04085"
     CDS             complement(<1..640)
                     /locus_tag="VGQ51_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035768.1"
                     /translation="MKIGAAFGKLLTGERIIREFLTVGIEDYHLVREKVYLQVGDSVL
                     DVSGNQWLLSLDPPVIGIWVEDKASTSLFVPGREFRLYFRDSAPPADGSPERNALSVL
                     SLEYADQLTEEDGVLFLLRVVSTTIHHISPIRARLLYEKFYKKPGVGFGRLKTIAAAY
                     SYPRRVRVISFQLEGNYNYIFPMDLLADIRSCGRFLFGMRHSNQVLKRIVDVK"
     gene            720..1817
                     /locus_tag="VGQ51_04090"
     CDS             720..1817
                     /locus_tag="VGQ51_04090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV9035769.1"
                     /translation="MKFVFLTYNISLHFNRAEDWFYHLRHSTGVYDALGKKHTVISIE
                     QINHTGRIERNGVDYHFLNYRKKKLYFPTRLHRYIKALSPDVIVVHGMHFPLQVLQLR
                     AQVGRHARIILQNHADQPGSGKRKILQTLADRSIDAYLFVSKEQGEQWVSRGIIANSG
                     KIREVMEASSLFIPQDRSLARSRTATSGDPLFLWVGRLDRNKDPLTVLEAFILFAKIH
                     PAARLYMVYHTEELLSEIREFVAATGVGDAVVLVGKIEHQQLQDWYNAAEFILSGSHY
                     EGSGIAVCEAMSCGCIPVVTDISSFRKMTGNGGCGILYPPGNKDALLAALLETRSMDK
                     VKERQKVLDQFRSELSFEAIAGGIDAVASSL"
     gene            complement(1748..2872)
                     /locus_tag="VGQ51_04095"
     CDS             complement(1748..2872)
                     /locus_tag="VGQ51_04095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9035770.1"
                     /translation="MRKNKPPLLVILSPGFPADEQDTSCLPAQQVFVRALNSNYPGLK
                     VVIIAFEYPKRKDRYSWSGNTVIALNGWKKGRMSKLLTCLAAWRQMNLLRRNTRMVGA
                     LSFWCTGCALTGKYYAKWKGLRHFAWILGQDARKGNVFIPLIRPVGGELVAMSNFLAG
                     EFERNYGIRPAHVIPNGVDPSLFTNRGMQRDIDLLGVGSLIPLKRYDLFITVVKELSR
                     DLPSICGVLCGTGPEQERLQQMIDRLGLDDRVRLAGGQSHQAALGFMQRSRILLHTSS
                     YEGFSTVCLEALYAGAHVISFCDAVGSPVKNWHVVTTIEEMIGLARKLLLDEGTVYES
                     VRLFTMDESVRQMMELFSYSDEATASMPPAMASKDSSDRN"
     gene            complement(2859..>3431)
                     /locus_tag="VGQ51_04100"
     CDS             complement(2859..>3431)
                     /locus_tag="VGQ51_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9035771.1"
                     /translation="LNAGEIIGLSGASGRGKTTIVNLVLGFFRPQEGEILINGQPANP
                     DKLRSYWTRVAYVQQHPFLLHDTILKNIVLSDERYDKDRLRDAVEAAGLQQMTSSQEE
                     GLNKLITENGKNISGGQRQRIAFARALYKDADLLILDEPFSELDETSEEEMLRYCRKL
                     AREGKIVLLITHNKKNLFLCHQTLELDEEE"
BASE COUNT          779 a          925 c          927 g          800 t
ORIGIN      
        1 ttttcacgtc gacgatcctt ttcagcacct ggttggaatg ccgcataccg aatagaaaac
       61 ggccgcagga ccggatatcg gccagcaggt ccatcggaaa gatatagttg taattcccct
      121 ccagctggaa ggagatgacc cgtacccttc gcggatagga gtaagcggcg gcaatagttt
      181 tcaacctccc gaaaccgacc cccggctttt tatagaattt ctcatagaga aggcgggccc
      241 ggattggcga gatgtgatgg atggtcgtgg atacgactct aaggagaaac aggactccgt
      301 cctcttcggt caactggtcc gcatattcca ggctcaatac gctgagcgca tttcgttcgg
      361 gggacccgtc cgcgggcgga gcggaatcac gaaaatacaa tctgaattcc ctgcccggga
      421 cgaaaagaga ggtggatgct ttatcctcca cccagatgcc gatgacggga gggtcgagac
      481 tgagcagcca ctggttaccc gagacatcca atacgctgtc gcccacctgc agatacactt
      541 tctcccgtac gagatggtag tcttcgatac cgaccgtcaa aaattcccgg atgatacgtt
      601 ctccggtgag tagctttccg aaagcagcgc ctattttcat atttgggtca ggtttgcaaa
      661 ggcatccctt tgcaaagaac gctcgttttg cttgaaagga tgccttaaat ttgggaccca
      721 tgaaattcgt tttcctcact tacaatattt ccctccattt caaccgtgcc gaagactggt
      781 tttatcatct ccggcatagt acgggcgttt acgatgcgct gggtaaaaag cataccgtta
      841 tcagtatcga gcagatcaac cataccggca gaatagaacg caacggcgtc gattatcatt
      901 ttttgaatta ccggaaaaaa aaattatact tccctacaag gttgcaccgg tatattaaag
      961 cgttatctcc cgatgtgatt gtcgtacatg gaatgcattt cccgctgcag gtgttgcagt
     1021 tgagggccca ggtgggccga cacgcccgga tcatccttca aaaccacgcg gatcagccgg
     1081 gcagcgggaa gagaaagatc cttcagaccc tggcggaccg ctcgatcgat gcctatctgt
     1141 tcgtgagcaa agaacaggga gaacaatggg tcagccgcgg catcatcgcc aactccggaa
     1201 agatacggga agtgatggaa gcctcttctc ttttcattcc tcaggaccga tcgttggcca
     1261 ggagccgtac ggcaacaagt ggagacccgc tgttcctatg ggtgggacgt ctcgaccgga
     1321 ataaagatcc gctgaccgtc ctggaggctt tcatcctctt tgcgaagatc catcctgccg
     1381 ctcgtttgta catggtctat cataccgaag aattgttatc ggagatcagg gagtttgtcg
     1441 cggcgaccgg cgtgggagat gccgtcgttc tggtaggaaa aatcgaacac caacagctgc
     1501 aggactggta taatgcggcc gaatttattc tttcgggctc gcactatgaa ggcagtggta
     1561 tcgccgtctg tgaagcgatg tcctgcgggt gcataccggt ggtcaccgat atttcttctt
     1621 tccgcaagat gacgggtaac ggcggctgcg gcatcctgta tccgcccggg aataaagacg
     1681 ccttgttggc cgccttgctg gagacccggt ctatggacaa ggtgaaagaa cgtcaaaaag
     1741 tgttggatca attccggtcg gaactgtcct tcgaagccat cgccgggggc atcgacgccg
     1801 tagcctcgtc gctgtaggag aagagttcca tcatttggcg gacactttca tccatggtga
     1861 acagccgcac ggattcatat accgttcctt catcgagcaa gagcttcctt gccaggccga
     1921 tcatttcttc gatggtcgtc acgacatgcc agttcttcac cggggaaccg acagcatcgc
     1981 aaaagctgat cacatgcgca ccggcataaa gggcttccag gcatacggtg ctaaagccct
     2041 cgtaggagga agtgtggagc aggatacggc tccgctgcat gaaccctagg gcggcctggt
     2101 gcgattgtcc cccggcgagc ctgacccggt cgtcgaggcc gagcctgtcg atcatttgct
     2161 gcaatctttc ctgttcaggg cccgttccgc agagcacccc gcaaatggaa ggcaggtccc
     2221 gggacaattc cttcacgact gtgatgaaga gatcataacg tttgagcggg atcagcgagc
     2281 cgacgccaag cagatcgata tcgcgctgca tcccgcggtt cgtgaagaga gaaggatcca
     2341 caccgttggg gatgacatgg gcgggacgta tgccataatt cctttcgaat tctccggcaa
     2401 gaaaattcga catggccacc agctcgccgc caaccggtct gatcagggga atgaagacat
     2461 taccctttct ggcgtcctgg cccaatatcc acgcgaaatg tcgcaggcct ttccattttg
     2521 catagtattt gccggtgagc gcgcagcccg tacaccagaa gctaagtgct cccaccatcc
     2581 tggtgttccg ccgaagaaga ttcatctggc gccatgcggc aaggcaggtc agcaacttgc
     2641 tcatacggcc ttttttccag ccgttgagcg caatgaccgt attgccggac caggaataac
     2701 ggtccttccg tttagggtat tcgaaagcga tgatcactac cttcaggccg ggatagttgc
     2761 tgttcaacgc cctcacaaag acttgctgag cgggcagaca ggacgtgtcc tgttcatcgg
     2821 cgggaaatcc cggggacagg atcaccagca acggcggttt attcttcctc atccagctcc
     2881 agcgtttggt gacaaaggaa aagatttttc ttattatgcg tgatcaacag cacgatcttt
     2941 ccctcccggg ccagcttccg gcaatagcgg agcatttctt cttccgaggt ttcgtccagc
     3001 tcgctgaaag gctcgtcgag gatcagcagg tcggcgtcct tataaagggc acgcgcaaag
     3061 gcgatccgtt gacgctgacc gccgctgatg ttctttccat tttccgtgat cagtttattc
     3121 aatccttcct cctgtgagga ggtcatctgt tgcaggcccg cagcttcgac ggcgtccctc
     3181 aatctgtctt tgtcatatcg ttcgtcgctg aggacgatat tcttcaggat cgtatcgtgg
     3241 agcaggaacg ggtgttgctg gacataggcg acgcgtgtcc agtacgagcg cagcttatcg
     3301 gggtttgccg gctgtccgtt gatgagtatc tctccttcct ggggccggaa aaagccgagc
     3361 accaaattga cgatagtggt tttacccctg ccggatgcgc cgctaagccc tattatctca
     3421 ccggcattca g
//
LOCUS       DASYSV010000127         4082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1304592, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000127 DASYSV010000000
VERSION     DASYSV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304592"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1020
                     /locus_tag="VGQ51_04105"
     CDS             37..1020
                     /locus_tag="VGQ51_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035772.1"
                     /translation="MITRENYEEFFLSYVDDELPVTVRAAVERFVADNPDLGPELEAL
                     LQCRVAPDHESGFRDKNILFQYESSLLSYVDGELEEPGRTSLEEWVQRHPGAARELRH
                     LQMTVSRPDLSIVCPDKESLYRDQRRRAVVLLWLRAGVAAAVLAGVALLLLPRQHSSG
                     TVAVAPKNISPAVTSAAPPALYSTVKDDRKNADTPVKARPARIVVKYAVRKQEDTKER
                     PVVVAPTVKNEVAIADPQPLKQQTITVEQNKIASAEVNIPKEQSSFATQALMQQEQEH
                     DNSIAEVSATPPKNKLRGLFRKVGRAFGTTAERDDDGQRQVLISAFQVALK"
     gene            1043..1882
                     /locus_tag="VGQ51_04110"
     CDS             1043..1882
                     /locus_tag="VGQ51_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV9035773.1"
                     /translation="MNRVFLLTAGLCIALSGRAQTDTTGTGNNTAPGSDTIHVGNLLI
                     VRDGKNDSSWHDDYGHHHHSYHNYHPSNITTTWCIVDLGYTNYNDMTNYASAATQAFA
                     PGSNKGWFTLNNGKSVDVNIWVFMQKINLVKHVVNLKYGVGVELNNYRYQDDIRYLIN
                     PTKVIMDTIHYHKNKLAEDFVTIPVMLNFNFTPHRRHPFGLSAGASFGYRYSSRQKFV
                     SAASGKQKTYNNFDLDSWKISYIGELNLGWFVLYGSYATKSIFTNGLNQVPYSVGIRI
                     GNW"
     gene            1978..2529
                     /locus_tag="VGQ51_04115"
     CDS             1978..2529
                     /locus_tag="VGQ51_04115"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEV9035774.1"
                     /translation="MTSRFPILCSLLVVSLISFRDAPTNSARHRLFHRTPEGEVYIVV
                     VKSNYELQLYDKDGWYATYPAVFGSKSLDDKMIMGDRKTPEGIFHIVSKRPHEKWNKI
                     MDLDYPTAEDVAKFNERKAEGLIPRTAKIGNGIAIHGTWPHDDNAVDAYQMWTNGCVS
                     LKNEDMDEVYRMTPVGTKVIIQH"
     gene            complement(2543..>4082)
                     /gene="acs"
                     /locus_tag="VGQ51_04120"
     CDS             complement(2543..>4082)
                     /gene="acs"
                     /locus_tag="VGQ51_04120"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEV9035775.1"
                     /translation="YMPMVPELAIAVLACARIGVIHSVVFGGFSAQSIADRIQDATCS
                     LVITSDGAFRGNKEIPLKSVIDDALVRCPSVNRVIVLTRTRTPISMIKGRDVWWEDEI
                     RKVETQGNPVCPAEEMDAEDPLFILYTSGSTGKPKGVVHTCGGYMVFTNYSFVNVFQY
                     NPGDVYFCTADIGWITGHSYIVYGPLSAGATTLLFEGIPTYPDAGRFWDIVDKFKVNV
                     LYTAPTAIRSLMGFGLDPVKNKDLSSLKVLGTVGEPINEEAWHWYDEHIGKGRCPIVD
                     TWWQTETGGILISNMAGITPGKPSFATLPLPGIQPMLVDDKGDEVQGNNVTGNLCIKF
                     PWPSIIRTTYGDHERCRKTYFATYPNLYFTGDGALRDEQGNYRITGRVDDVLNVSGHR
                     IGTAEVENAINMHTGVVESAVVGYSHDIKGQGIYAYVIFNGTKSDDESIKAGIVQTVA
                     RIIGPIAKPDKIQLVSGLPKTRSGKIMRRILRKIAEGELQNLGDTTTLLDPAVVEEIK
                     KGRL"
BASE COUNT         1012 a         1101 c         1067 g          902 t
ORIGIN      
        1 atacctggta aaggtggaaa atttaatttg aggctcatga tcaccagaga aaattatgaa
       61 gaattctttt tatcgtacgt cgacgacgag ttgccggtga cggtccgcgc cgcggtggaa
      121 cgattcgtcg ccgacaatcc agatctgggt ccggaactgg aggcattgct gcaatgccgt
      181 gtcgcgccgg accacgaatc cgggttccgg gataagaaca tccttttcca atatgaatcg
      241 tcattgctgt cctatgtcga cggagagctg gaagagccgg gacgcacttc gctggaagaa
      301 tgggtgcagc gccatccggg tgcggcgcgc gagcttcggc atttacagat gaccgtgagt
      361 cgccccgatc tttcgatcgt ctgccccgac aaggaaagcc tgtatcggga tcagcgccgg
      421 agggcggtcg tccttctttg gctgagggcg ggtgtagctg ccgcggtgct ggcaggcgta
      481 gcgctattac tcttgccgcg acaacattcc tccgggaccg tggccgtggc gccgaaaaat
      541 atttctccgg ctgtaacttc ggctgctcca cccgcgttat actctacagt aaaagatgat
      601 cggaagaatg cggatacacc ggtgaaagcc cggccggcgc gcatagtggt gaagtatgcg
      661 gtgcggaagc aggaagatac caaagaaaga ccagtcgttg tggctcctac ggttaagaac
      721 gaggtcgcca tcgccgatcc ccaaccgctg aagcaacaga cgataacggt ggagcagaac
      781 aaaattgcaa gtgcggaggt gaatatcccg aaggagcagt cttctttcgc cacccaggcc
      841 ttaatgcagc aggaacagga acacgacaat agtatcgccg aagtatcggc gacgccgcca
      901 aagaataaat tacgcgggct cttcagaaaa gtgggccgcg ctttcggaac gacggcggaa
      961 cgcgacgacg acggtcagcg acaggtcctg atcagcgctt tccaggtcgc attgaaataa
     1021 ttctcattaa aaaaacaaat gtatgaaccg ggtattctta ctaacggccg gattatgtat
     1081 tgccctttcc ggccgggcac agaccgatac gacaggcacg ggaaataaca cggctccggg
     1141 atccgatacc attcacgtcg gcaacctcct gatcgtccgc gacggaaaaa acgattccag
     1201 ctggcatgat gattatggac accatcacca tagttatcac aattatcatc cttccaatat
     1261 cacgaccacc tggtgtatcg tcgatctggg gtacaccaat tacaacgata tgaccaatta
     1321 cgcttccgcc gcgacccagg cattcgcgcc ggggtctaac aaagggtggt tcacactcaa
     1381 taatggcaaa tccgtggacg tgaatatctg ggtctttatg caaaagatca acctggtcaa
     1441 acatgtcgtc aatctgaaat atggggtggg cgtcgaactg aacaactacc gttaccagga
     1501 tgatatccga tatctcatca accccactaa agtgatcatg gataccatcc attaccataa
     1561 gaacaaactc gcggaggatt tcgtcactat tcccgtgatg ctcaatttca atttcacccc
     1621 gcatcgtcgt cacccgtttg gattgagcgc gggcgccagt tttgggtaca ggtattcttc
     1681 gcggcagaaa ttcgtcagcg ctgcatccgg caaacaaaag acatacaata atttcgatct
     1741 cgattcctgg aagatttcct acatcggaga gctgaatctt ggatggttcg tgctatatgg
     1801 cagctatgcc acgaaaagca tatttaccaa tggcctgaac caggtgccgt acagtgtggg
     1861 gatccggatc gggaattggt aagccgccgc cccgccgccg ctgcagctgt taaagttttt
     1921 tcacatcaaa ataatcgggg ctcgcgtcac gttggaaagg gttaaaacca tatcatcatg
     1981 acgtcccgat tcccgatctt gtgcagtctg ttggtagtca gcctgatatc tttccgtgat
     2041 gcacccacga attcagcccg tcaccgtctt tttcatcgta ctcccgaagg agaggtctat
     2101 atcgtcgtcg tcaagtccaa ttatgagctg cagctgtacg acaaggatgg ctggtatgcc
     2161 acatacccag ccgtattcgg aagcaaatcc ctggatgaca agatgataat gggagaccgc
     2221 aagacgcccg aaggcatctt tcacatcgtc agcaaacgac cgcatgagaa atggaacaag
     2281 atcatggacc tcgattatcc cacggcggag gacgtcgcca aattcaacga acgcaaggcg
     2341 gagggtctta tcccccggac ggcaaagatc ggtaacggca tcgctataca cggcacctgg
     2401 cctcacgacg ataatgcggt ggatgcctac cagatgtgga ccaatggatg cgtctcgcta
     2461 aagaatgaag atatggatga ggtgtaccgt atgacgccgg tggggacgaa agtgatcata
     2521 caacattagc cggggcggct cgctagagcc tccccttctt tatttcttcc accacagccg
     2581 gatcgagcaa ggtggtcgta tctcccaaat tctgtaactc cccttccgca atcttccgca
     2641 gaatgcgtcg catgatcttt ccgctgcgcg tcttcggcag accactgacg agctggatct
     2701 tgtcgggctt ggcgatcggc ccgatgatcc tggcgaccgt ttgtacgatc cccgccttta
     2761 tggactcatc gtccgatttg gtaccattga agataacata ggcatagatc ccctgtccct
     2821 tgatatcgtg agaatagccg accacggcgc tttcgacgac acctgtatgc atattgatag
     2881 cattctcgac ttccgcagtg ccgatacggt gtccgctgac attgagaacg tcgtcgacgc
     2941 gcccggtgat ccgataatta ccttgctcat cacgaagtgc gccatcgccc gtaaaataga
     3001 gattgggata ggtggcgaaa tatgtcttac ggcaacgctc atggtcgcca taagtcgtcc
     3061 ggatgataga aggccaggga aatttgatac atagattacc ggtgacattg ttcccctgca
     3121 cttcgtcgcc tttgtcatcg accagcatag gttgtatacc cggcaggggc agggtcgcaa
     3181 aggacggctt ccccggggta atacccgcca tattcgagat caaaataccg cctgtctccg
     3241 tttgccacca ggtatccacg atgggacagc ggccttttcc gatatgctcg tcataccaat
     3301 gccaggcttc ttcattgatc ggctcgccta cagtccccag cactttgagc gagctcaggt
     3361 ccttgttctt taccgggtcg agaccgaaac ccatcaggct gcggatcgcg gtgggagcgg
     3421 tataaaggac attgaccttg aatttgtcta cgatatccca gaaacggccg gcatccggat
     3481 aggtcggaat gccctcgaac agaagcgtgg tggcaccagc gctcagcggt ccataaacga
     3541 tataactatg tcccgttatc cagccgatat ccgccgtgca gaaatacacg tcaccgggat
     3601 tgtactggaa tacattgaca aaactatagt tagtaaagac catatagccg ccgcaggtat
     3661 gtacgacgcc tttgggcttc ccagtagacc ccgaagtata taaaataaaa agcgggtcct
     3721 ccgcatccat ctcctctgcc ggacagacag ggttgccctg cgtctcgacc ttgcggatct
     3781 catcttccca ccacacgtcg cgacccttga tcatgctgat aggagtacgg gttcgcgtga
     3841 gaacgattac cctgttgacg gaggggcacc gcaccaacgc atcgtcgatc acggatttca
     3901 aagggatctc tttattaccc cggaaggctc cgtcggaagt gatgaccagc gaacaggtcg
     3961 cgtcctgtat cctgtcggca atgctttgtg cggagaaccc tccaaagaca acagagtgta
     4021 taacgccgat gcgcgcgcag gcaaggacgg cgatggccag ctcaggcacc atcggcatat
     4081 ag
//
LOCUS       DASYSV010000128         4364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_664201, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000128 DASYSV010000000
VERSION     DASYSV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664201"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VGQ51_04125"
     CDS             <1..491
                     /locus_tag="VGQ51_04125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEV9035776.1"
                     /translation="AQACIYGYMKKSESEKAAGNKKIGLEFLAANKNKPGVVTLPSGL
                     QYQVIKEGTGPKPALTDRVKVHYHGTTIDGKVFDSSIQRGQPVELAVNGVIPGWTEAL
                     QLMSVGSKWKLFIPSDLAYGDQQAGPLIPPGSALVFDIELLDIVGKPVPPPLPHDSTT
                     KQ"
     gene            complement(462..3041)
                     /locus_tag="VGQ51_04130"
     CDS             complement(462..3041)
                     /locus_tag="VGQ51_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEV9035777.1"
                     /translation="MHRINFLFFLPAMWLGFAREGICQGVGPGVPYSLAVYRSAVLRD
                     IRYSLDFRLPAERESPVRGEEKLSFRLDSAWSGEAVLLDFRTPDKTAESLRINGVSAD
                     ARCINEHLVLPAGLLHKGLNTVAVDFTAGDPALNRNREYLYTLLVPDRARTLFPCFDQ
                     PDLKAVFELTLVAPAGWAVMANAPVLDSMALGDSCRWRFRPSNRISTYLFSFVAGKWS
                     SAVRIVDGRPMRFLYRETDSTKIRLSLDSIFRIEGQALRFMEEYTGIRYPFQKFDFVA
                     IPDFQFGGMEHVGAIQYKASSLFLDSGATREQLIGRSNLLSHETEHMWFGDLVTMRWF
                     NDVWMKEVFANFMADKIGNVTLPDGKYELKFLTDHYPAAYSIDRTEGTHPIRQELDNL
                     QEAGSLYGNIIYHKAPIVMRQLEALMGAEAFRNGLRDYLRDYAGGNASWPDLIRALQR
                     YTKIDLHNWNEVWVNGAGRPVFSYDCGINRKSRRITSFTIHQKGEDGSDRVWPQLFSV
                     ALVYADRMEQLMVRMNDSAVRLDPAIGKDLPVFVILDADGRGYGLFPPSSIYMRRADD
                     SAFYKTSPVFRAALYVNRYESRLADTVSSPGISNVDLDLVRREPEELNLNLLLDEMTS
                     IWWRFESPTMRTPLAPILESTYWGTMRHAHSDNAKKLLFRAYSNIVLTRAGQDTLFTI
                     WKEQRPPVGVKLSEDDYTNLAAALAIREYPGWRDILKEQLGRIHNPDRRERWLFLQPA
                     LSNDTAERDGFFAMLHDPQVRKKEAWVLAGLSYLHHPLRTGYSEKYLPATLELLEDIQ
                     RTGDVFFPQSWLQTSLSYYRTASAAAIVRVFLQTHPNYNPRLKAKILQAADNLLFRRT
                     IVR"
     gene            complement(3066..>4364)
                     /locus_tag="VGQ51_04135"
     CDS             complement(3066..>4364)
                     /locus_tag="VGQ51_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEV9035778.1"
                     /translation="SIELRTELSGFADKATHWKEVREKLKAQIQEEEKKNSRTGGALV
                     LQLMSPTAGPCDFTVSYLTMAAYWQPSYDLKAGGGTDSLHLLYKANLVQTSGIDWRRV
                     KLSLSTAMPTQSGNAPILQAWFLRFVEPVAAGYFLNSAQPSNSVAANAPTIMLRGTQS
                     LKEAALPMYVVDGKIMSEAEFKTIDSRAIKSINVLKGEAATAIYGSRAANGVIQIMMK
                     DEPGDYVSLNEQQMDAVFDIDIPYDVAGNGKEQSVELKDYRLPCTYQYYAAPRADRDA
                     YLLGQVTGWEKLNLLPGEASIMVDGTYIGKSNIDPGSLQDTLNLTLGKDKRIVIKKEK
                     VTDFSSVKFLGSNKKQVFTYEITVRNNKKEKASLTLKDQYPISTDKEIESELLESSGA
                     TVNTDTGILTWKVELAPGESRKLRISYSVKYPKDKSISMN"
BASE COUNT          997 a         1348 c         1042 g          977 t
ORIGIN      
        1 aggcccaggc ctgcatttac ggctatatga aaaagagcga gtccgaaaaa gcagccggca
       61 acaaaaagat cggactggaa ttcctcgccg ccaataaaaa taagccaggc gtggttacgc
      121 tccccagcgg tctgcaatac caggtcatca aggaaggcac cggcccgaaa ccggcgctca
      181 ccgacagggt gaaggttcat tatcacggca ccaccatcga tgggaaggtc ttcgatagca
      241 gcatccaaag aggccaaccg gtcgagctgg ccgtcaatgg cgtaatcccc ggctggacgg
      301 aagcgttaca gctcatgtcc gttggcagca aatggaagct cttcattcct tccgatctgg
      361 cttatggcga ccagcaggcc ggtccgttga tcccgccggg ctccgctctc gtatttgata
      421 tcgaattgct ggatatcgtc ggcaaaccgg tgcccccgcc gctaccgcac gatagtacga
      481 cgaaacagta aattgtccgc ggcctggagg atcttcgcct tcagccgcgg attataattt
      541 ggatgcgtct gtaaaaacac gcgcacgatc gccgctgcac tcgccgtccg gtaatagctg
      601 agactcgtct gcagccagct ctgcgggaaa aagacatccc ccgtacgctg gatatcctcc
      661 agcaattcga gcgtcgccgg taaatatttt tccgaatatc ctgtcctcaa ggggtgatgc
      721 agataggaca atcccgccaa cacccaggcc tctttttttc tgacctgagg atcatgcaac
      781 atcgcaaaaa acccatcgcg ttcggccgtg tcgtttgaca atgccggttg caggaacagc
      841 caccgttcac gacgatcggg attgtgaata cgccccaatt gctccttcag gatatcccgc
      901 caccccgggt actcgcgtat agccagcgcc gccgccagat tcgtataatc gtcttcggat
      961 aattttacgc ccaccggagg tcgttgctct ttccagatcg taaataatgt atcctgcccc
     1021 gcccgggtca gcacgatatt gctatacgcg cggaaaagga gtttctttgc attgtccgaa
     1081 tgggcgtgac gcatggttcc ccaataggta ctttccagga ttggcgccag tggggtccgc
     1141 atggttggag actcgaaacg ccaccagatg gaggtcattt catccagcag gagattcagg
     1201 ttcagctcct ccggctcccg cctcaccaga tccaggtcca cattcgagat acccggcgac
     1261 gaaactgtgt ccgccaacct gctttcatat cggttgacat agagcgccgc acggaacaca
     1321 gggctggtct tataaaaagc gctatcatcg gctctacgca tatagatact gctgggcggg
     1381 aacaatccat atccacgtcc atccgcatcc aggatcacga agaccgggag gtctttaccg
     1441 atagccgggt ccagccgaac cgcggaatca ttcatccgga ccatcagttg ctccatcctg
     1501 tcggcgtaca ccaatgccac ggaaaataac tgcggccaga cccgatccga cccatcctcc
     1561 cctttctgat ggatcgtgaa cgaagtgatc ctcctgctct tcctgtttat gccacaatca
     1621 tagctgaata ccggtctccc cgcaccattc acccaaactt cattccagtt atgaagatcg
     1681 atcttcgtat atcgctgcaa ggcccggatc agatcgggcc atgatgcatt cccacccgcg
     1741 taatcgcgca aataatcccg aagaccgttc ctaaaggcct ccgcccccat cagggcttcc
     1801 agctgccgca tgacgatcgg cgctttgtga tagatgatat tcccgtataa tgatccggct
     1861 tcctgcagat tgtccagctc ctgccggatg ggatgcgttc cctccgtccg gtcgatacta
     1921 taggccgcgg gataatgatc cgtcaggaac ttcagctcat atttaccatc gggcagcgtc
     1981 acattcccga tcttgtcggc catgaaattc gcgaacactt ccttcatcca tacgtcattg
     2041 aaccagcgca tcgtcaccag atcgccgaac cacatgtgct ccgtctcatg agataacaga
     2101 ttgctcctgc cgatcaattg ctcgcgcgtc gcgcccgaat cgaggaacaa tgaggaagcc
     2161 ttgtattgta tagcccccac atgctccatg cccccgaatt gaaaatcggg tatcgctaca
     2221 aaatcgaact tttgaaaagg atagcggatg cctgtatatt cctccatgaa ccgtaacgcc
     2281 tgtccctcta tccggaatat cgaatcgaga ctgagcctga tcttcgtact atccgtctcg
     2341 cgatacagga atcgcattgg acgaccatcg acgatcctca cggccgacga ccattttccg
     2401 gcgacaaaag aaaaaagata cgtgctgatc ctgttcgatg gccggaaacg ccagcgacag
     2461 ctgtcaccca aagccatgga atccagcaca ggggcattgg ccatgacggc ccatcccgcc
     2521 ggcgccacca gcgttaattc gaaaacggcc ttgagatcgg gctgatcgaa acaagggaac
     2581 aacgtacgcg cccggtccgg caccagcagg gtatacaggt actcgcggtt acggttaagc
     2641 gccgggtcgc ccgccgtgaa atcgaccgca acggtattga ggcctttatg cagcaacccc
     2701 gccggcagga cgaggtgctc attgatacaa cgggcatccg cagagactcc attgatccgt
     2761 aaactctcag cagtcttatc gggcgtcctg aaatccaata gcaccgcctc cccgctccac
     2821 gccgaatcca gccggaagga taatttctcc tctccgcgaa ccggactctc ccgctccgcg
     2881 ggcaaccgaa agtccaacga ataccggata tcccgtaata ctgcgctccg atagaccgcc
     2941 agcgaataag gcaccccggg gccaacgccc tgacagatcc cttcccgggc aaaacccagc
     3001 cacatcgccg gcaagaagaa aagaaagttg atcctatgca tgagcgtttg gtcgcccaat
     3061 atacgctaat tcatactgat ggatttatcc ttgggatatt ttacgctata gctgatccgc
     3121 aacttccggc tctcgcccgg agccagctcc accttccatg tcaggatgcc tgtatcggta
     3181 ttgacggtcg cgccgctgct ttccagcaac tcgctctcga tctccttatc cgtactgatc
     3241 ggatattgat ccttcagggt aagactggcc ttttctttct tgttattccg gacggtgatc
     3301 tcataagtga acacctgctt cttattcgaa cccaggaatt tcacactgct gaaatccgtc
     3361 accttctcct ttttaatgac gatgcgtttg tcttttccca gcgtcaggtt cagcgtatcc
     3421 tgcagcgagc ccggatcgat atttgatttt ccaatatacg tcccatctac cataatgctc
     3481 gcttctcccg gcaggaggtt cagcttctcc caccccgtca cctggcccag cagatacgca
     3541 tcccggtccg cccgcggggc tgcataatac tgataggtgc atggcagccg gtagtctttc
     3601 aattccacac tctgctcttt cccgttgccc gcgacgtcat agggaatatc gatatcgaag
     3661 acggcatcca tctgctgctc attcagcgaa acataatcgc ccggctcatc cttcatcatg
     3721 atctgtatca cgccgttcgc cgcacgcgag ccataaatag ccgtcgcagc ctctcccttc
     3781 agcacattga tcgatttgat cgcccggctg tcgatcgttt tgaattccgc ctcggacatg
     3841 atcttcccat cgacgacata catgggtaag gcggcttcct tcagcgattg cgttcctctt
     3901 agcattatgg tcggtgcatt cgcggcaacc gaattcgacg gctgagccga attcaaaaaa
     3961 tatcccgcgg caaccggctc cacgaaccgc agaaaccagg cctgcagtat cggcgcattc
     4021 ccgctctgcg tgggcattgc ggtggacaga ctcagcttta cccgtcgcca gtcgatgccc
     4081 gatgtctgca ccaggttggc cttataaagg agatgaagcg aatccgtgcc gccccccgct
     4141 ttgaggtcat atgaaggctg ccagtacgcc gccatcgtca gatatgaaac agtaaaatca
     4201 caaggccccg ccgtcgggga catcagttgc agcaccagcg cgcctcctgt ccgggaattc
     4261 tttttttcct cttcctgtat ctgcgccttc agcttttccc gaacttcctt ccaatgagta
     4321 gccttgtccg caaagccgct gagttccgtt cgcagctcga tcga
//
LOCUS       DASYSV010000129         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_922343, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000129 DASYSV010000000
VERSION     DASYSV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922343"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /gene="ligD"
                     /locus_tag="VGQ51_04140"
     CDS             complement(<1..1166)
                     /gene="ligD"
                     /locus_tag="VGQ51_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEV9035779.1"
                     /translation="MLATLVDSPPSDAGWIYEVKWDGYRAIARCDKSDVRLISRNNKS
                     FNDKFYPIREALTALKLRAVLDGEITVLQPNGASSFSALQGWRSEADGDLVYYVFDLL
                     WLDGKDLTSLPLSRRRALLTRLLPAEGPIRESHSFDTSATKFLATAKKLGLEGIIAKR
                     ADSPYRPGDRGRDWLKIKVQRRQEVVIGGYTKNDDSPKLFSALLIGVYDKDGLHYIGK
                     VGTGFNETLQREMMKKFKPLIVSRSPFTIVPNVNEPSRFRPDPPHAEAFWLKPRLVCE
                     VSFAEMTRDGSMRHPSFEGLREDKDPKDVHAELAKPVAAATLTEKPPRTKAEKPPRTN
                     GKKPPRTNLKKAPAPDRTHKTGSGRTLELLRPAGSSERRTLLNPKDQRQVRVVSG"
     gene            1341..1520
                     /locus_tag="VGQ51_04145"
     CDS             1341..1520
                     /locus_tag="VGQ51_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035780.1"
                     /translation="MGIIEQLAEIKYEEGIEKGLEKAVRVLLVNTEFSSAKIARLVDV
                     PVARVRRIKKELNGK"
     gene            complement(1584..>3335)
                     /locus_tag="VGQ51_04150"
     CDS             complement(1584..>3335)
                     /locus_tag="VGQ51_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV9035781.1"
                     /translation="RTRVSKVQYLLITAALTGLVSGVMAVFLKRSVQQVEKLVQHFSG
                     KEYLFLLCPAAGLLLTTWIIQRWFRGHIDKGIAMVLKAIARKSSFIPSRNNYIHFITS
                     AITVGSGGSAGLESPIIATGSSLGSTMGRLGLLNYPERTLMIACGAAAGIASIYDAPI
                     AGVMFAVEVLLAETVVSYFVPLIIAAVVGVLCSKIYIGGPSMFNFALKQDFNYRNVPM
                     YVLMGVGAGFLSLYYAKAFRGVDKKLHAWKTGPYTKALAGGLMLAAFFLLFAPLYGEG
                     YESARMLANDTPNRILPTAGWLHGIVPQDVLLVIFTGCILLLKPITAAITIGSGGNGG
                     NFAPSVFVGAYWGFFFSKLTNVTCWPWLHLPETNFTLVGMAGLLSGVMYCPLTAIFLI
                     AEITNGYGLIIPLMLVSCIAYFIARTFEPYYMETKQLAMDGLIFTHRREGNILSALSL
                     ENLIERDHQTLRPEDGFGDLIGHIRESDKQIFAIIDAKHKLAGVITLNDIRGKMFQPE
                     LYDQLLLKEIMRQPAAVLPLDSAMTQVMHEFDTTKARFLPVVDEEQRFVGFISRTRLF
                     EQYRSRVAQQKDIYEDE"
BASE COUNT          772 a          912 c          932 g          719 t
ORIGIN      
        1 ccgctgacga cgcggacctg tcgctggtcc tttgggttca gaagggtccg gcgttcggag
       61 gatccggcgg ggcggagaag ttctaaggtt cggccggagc cggtcttgtg cgttctgtct
      121 ggcgccgggg ccttctttag gttggtgcga gggggcttct ttccgttggt gcgggggggc
      181 ttctctgcct tggtgcgagg gggcttctcc gtcagcgtcg ccgcggctac cggcttcgcc
      241 aattccgcat gcacgtcttt cggatcctta tcttccctca gcccctcgaa agaaggatgc
      301 cgcatcgatc cgtcgcgtgt catctcggcg aagctaactt cgcataccag ccggggtttg
      361 agccagaagg cttcggcatg gggcggatcc ggccggaagc gcgagggctc gttgacgttg
      421 ggcacgatcg taaacggaga gcgggagaca atcagcggtt tgaatttctt catcatctcg
      481 cgctgcagtg tttcattaaa acccgtgccc acctttccga tataatgcag cccgtccttg
      541 tcatacacgc cgatcaacag cgcgctgaac agtttggggg aatcatcatt cttcgtatac
      601 ccgccgataa cgacctcctg acgccgttgc actttgatct tcagccaatc gcgcccccgg
      661 tcaccgggtc ggtacgggct gtcggcgcgt ttggcaatga tgccttccaa acccagcttt
      721 ttcgcggttg caaggaattt ggtggcggaa gtgtcgaaag aatggctttc ccgaatgggt
      781 ccttcagcag gcaggagacg agtcagcaaa gcgcgccgcc gcgatagggg gaggctcgtc
      841 aggtcctttc cgtccaacca tagcaggtcg aaaacataat agacaaggtc gccgtccgcc
      901 tcactccgcc aaccctgcag ggcgctgaaa ctggaagcgc catttggctg caggacggta
      961 atctcaccgt caaggaccgc gcgaagcttc agcgcggtaa gcgcttcccg gatcgggtag
     1021 aatttatcgt tgaaagattt attgttccgg gagatcagcc ggacatccga tttgtcacac
     1081 cgggcgatgg cccgatagcc gtcccatttt acctcataga tccatcctgc atctgacgga
     1141 gggctgtcca ccagtgtggc cagcatggga ctgatcgtta gtggcgggcg agtcacaatt
     1201 atttctctaa aaatcttatg ccaaacctat gcaaattata gtcctaaagg ccggagagtt
     1261 tcccaggtcg cttaatgagt tggcgtgaat tatttatctt tatggaccgg gcaagtaaga
     1321 tttttcaaaa aaagactact atgggtatca tcgaacaatt ggcggagata aagtacgagg
     1381 aaggcataga gaagggcctg gaaaaagccg tccgcgtcct tcttgtcaat accgaattct
     1441 caagcgcgaa gatcgccagg ctcgtggacg tgccagttgc cagggtaagg agaataaaga
     1501 aagaactgaa cggaaaatag ttccgcctca acgctcagga gagccgtcga ggtcgggcca
     1561 ggagagcctg agaggtcggg agcttactca tcctcataga tatccttctg ctgcgcgact
     1621 ctcgaccggt actgttcaaa cagtcttgtc ctcgaaataa atccgacgaa ccgctgttcc
     1681 tcatccacca ccggcagaaa cctggccttc gtcgtatcga attcgtgcat cacctgcgtc
     1741 atagcgctat ccagcggcag gacagctgca ggctgtcgca tgatctcctt taacaatagc
     1801 tggtcataca gttccggttg gaacatcttc cctcggatgt cgttcagcgt aataacaccg
     1861 gccagcttat gtttggcgtc gatgatagcg aatatttgtt tgtcgctttc ccggatatgg
     1921 ccgatcagat cgccgaaccc atcttccgga cgcagtgttt ggtgatcccg ttcgatcagg
     1981 ttttcaaggc tcaacgcgga gaggatattg ccctcccggc gatgcgtgaa tatcagcccg
     2041 tccatcgcca gctgtttggt ctccatatag tagggctcga aagtacgcgc gataaaatag
     2101 gcgatacacg atacaagcat cagcgggatg atcagtccat agccattggt gatctcggcg
     2161 atcaggaaga tggcggtcag cgggcaatac attacgccgc tgagcagtcc agccatgccc
     2221 accagcgtaa aattggtctc gggcaggtga agccagggcc agcaggtgac attcgtgagc
     2281 tttgaaaaaa agaaacccca atatgcccca acgaataccg aaggggcgaa attgccgcca
     2341 ttccctcccg atccgatggt aatggccgcg gtaatcggtt tcaacagaag gatgcaacct
     2401 gtgaagatga ctaacaggac gtcctgcggg acaatgccat gcagccagcc cgctgttgga
     2461 aggatgcggt tcggagtatc gttggccagc atccgggcgc tctcgtaacc ctccccgtat
     2521 aacggggcaa aaagaaggaa aaaggctgcc agcatcaggc cgccggccag cgccttggta
     2581 tagggtccgg tcttccatgc atgcagcttc ttatcgacgc cgcgaaaggc tttcgcataa
     2641 tacagcgaca agaaacccgc tccgacaccc atcaggacat acatcggcac gttccggtag
     2701 ttgaaatcct gtttcagggc aaagttgaac atgctgggtc ccccgatata tatcttcgaa
     2761 caaagtacgc cgacgacggc ggctatgatc aacgggacaa aatagctgac caccgtctcg
     2821 gcaagcaaca cttcgacggc aaacatgacg cccgcaatgg gcgcgtcgta tatcgaggca
     2881 attccggcag cggcgccgca agcgatcatc agggtacgtt ccggatagtt cagcagacca
     2941 agacggccca tcgtcgaccc cagcgaggag cccgtggcaa tgatagggga ttccaaccca
     3001 gccgatccgc ccgatcccac ggtgatagcg ctcgtgatga aatggatgta attattacgc
     3061 gaaggaataa aagaggattt gcgcgcaatg gccttcagca ccatggcaat ccctttgtcg
     3121 atatgccccc tgaaccaacg ctggatgatc catgtcgtca gcagtaatcc cgccgccggg
     3181 caaagcagga acaaatattc tttgccgctg aaatgctgta ccagcttttc cacctgctgt
     3241 acagagcgct tcaggaaaac cgccatgaca cccgagacaa gtccggtcag ggcggcggtg
     3301 atgaggaggt attgtacttt tgatacgcgt gtacg
//
LOCUS       DASYSV010000130         4111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1432292, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000130 DASYSV010000000
VERSION     DASYSV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432292"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VGQ51_04155"
     CDS             <1..475
                     /locus_tag="VGQ51_04155"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEV9035782.1"
                     /translation="QKAFLLRHGFNDGICFLVDMRLPSGKNRFFVYDLHSDSILLAGI
                     VAHGCGRRNFSPTPEFSNAEGSNCTALGRYRIGYPYKGQFGLAYKLYGLDSTNDQAFN
                     RNIVLHSYGAVPFEETYPTPICNSLGCPMVSPRFLNNLQPLIDHSRKPVCLWIFN"
     gene            complement(472..1185)
                     /locus_tag="VGQ51_04160"
     CDS             complement(472..1185)
                     /locus_tag="VGQ51_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEV9035783.1"
                     /translation="MTTIFHPAAGRGHVNFGWLDSHHSFSFGNWHDREKVQFGALRVL
                     NDDVVKGGGGFDTHKHENMEIISIPLKGALAHKDSTGTDGIIYSGDVQIMGAGTGITH
                     SEYNASHYDPVNFLQIWIFPRQNGGKPRHEQKTFDAAARAGHWQVIVSPRAEDGALRI
                     NQDARLALARVGAGETLEYRTVFPGNGIYLFVVYGEIEAAGQALQQRDGLAISGVDVV
                     ALRAVSGAEVLAIEVPLFG"
     gene            complement(1201..2001)
                     /locus_tag="VGQ51_04165"
     CDS             complement(1201..2001)
                     /locus_tag="VGQ51_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV9035784.1"
                     /translation="MTPYQIKLPQFEGPFDLLLFFIERDELDIYNIPIHNIIQDFLTY
                     IHSQESLNIELSSEFILFISTLMRIKAKMLLPRKELDAEGNEIDPRQELIDKLLEYKR
                     YKEAAATLAEMEAIRHLMVRRGNLQKELSGIGEEAGEGTEIQAITLFKLMKTFEKVMQ
                     RIQQRQNRPVHTVVQYNYTMEGSRKHILTTVREQRTLSFERLFEVCRDRLQAIFIFLS
                     MLELVQLNYMSILIGEGRNNFIVEYNADKPSDPVEEVVLTGPVDPLAN"
     gene            complement(2163..3155)
                     /locus_tag="VGQ51_04170"
     CDS             complement(2163..3155)
                     /locus_tag="VGQ51_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HEV9035785.1"
                     /translation="MTNHCARRWLIVFLFTVGFTTFLQAQNDLQKAVSGAAITKDPKD
                     TTKMTWKTGGLINLTFNQTALSNWSAGGDKSALAFNGLVNLYAFYLDGRRSWDNFLVV
                     GYGIANTTSLGTRKTDDRFNITSKYGYDLGHKWYLTGLFDFRTQFAPGYNYPDATHQV
                     LTSDFLAPAYLLFSIGMDYKPTDNFSLFLSPATVRELIVDNDSLAAHGAFGVDSGQKS
                     RFEFGAYASINYNSPLSKTASYTGRLDLFSDYLNNPQNIALYMTNVLNVKVTTLLSMN
                     LALTLVYDDRIHSVKEDGTFGGPRLQLQEVLGVGLAYKFAKKKREGSEAVKIAQ"
     gene            complement(3370..3750)
                     /gene="mscL"
                     /locus_tag="VGQ51_04175"
     CDS             complement(3370..3750)
                     /gene="mscL"
                     /locus_tag="VGQ51_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV9035786.1"
                     /translation="MAFLKEFRDFVMRGSVIDLAVAVIVGAAFTAIVTSLTDNVITPL
                     LLAPALKAAHLSDIDKLAWGAVKYGLFLSAVLKFIIFALILFLFIKGIHSLHKKKAAA
                     PAEPSSTDKLLMEIRDELKKGKAA"
     gene            complement(3777..>4111)
                     /locus_tag="VGQ51_04180"
     CDS             complement(3777..>4111)
                     /locus_tag="VGQ51_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HEV9035787.1"
                     /translation="INHFERLLIGHNDTHILKFYLHISIDEQKHRLEERIRNPSKQWK
                     YNEDDFKEAKLWDEYMEAYEDCFENCSEVPWKIVPADQNWYKEYLIAHTLRDTLRDLN
                     MQYPGLKK"
BASE COUNT          924 a         1213 c          983 g          991 t
ORIGIN      
        1 gcaaaaagcc tttctccttc gccacggctt caacgacggc atttgttttc tcgtcgatat
       61 gcgtcttcct tccggcaaga accgtttttt cgtttatgac ctgcacagcg attccatcct
      121 gctggcaggc atcgtcgcgc atggatgcgg ccgcaggaac tttagtccga cgcccgaatt
      181 ctccaacgcc gaaggaagca actgtaccgc gctcggaaga taccgcatcg gttatcccta
      241 caagggacaa tttggactcg catataaatt gtatggattg gacagcacca atgaccaggc
      301 attcaaccgg aatatcgtct tacatagtta tggtgcggtg cctttcgaag agacttaccc
      361 cacccccatc tgcaacagcc tgggctgtcc gatggtgtcg ccgcggtttc tgaacaacct
      421 gcaacccctg atagatcatt cgcgaaaacc ggtatgtctg tggatcttta actaaccgaa
      481 tagcggcacc tctatcgcca gcacctccgc gcccgatact gcacgaagcg caaccacatc
      541 gactccgcta atggccagcc catcgcgttg ctgcagcgcc tgacccgccg cctctatttc
      601 tccgtaaact acaaaaagat agatcccatt cccggggaat acggtcctat actccagcgt
      661 ctccccggca ccgacccggg ccaacgccag tcgcgcatcc tgattgatcc gaagcgcccc
      721 gtcttccgcc cgcggactga cgatcacctg ccagtgaccg gcacgcgcag cggcatcgaa
      781 tgtcttctgt tcatgacgtg gcttcccacc gttctgtctc ggaaagatcc agatctgcag
      841 gaaattgacg ggatcgtaat gcgaagcatt gtattctgaa tgggtaatgc cggtgccggc
      901 gcccatgatc tgtacatcgc cgctatagat gatgccgtcg gtgcccgtgc tgtccttatg
      961 ggcaagcgcg cccttcagcg gaatggagat gatctccatg ttctcgtgct tgtgggtatc
     1021 gaaaccgccg ccgcctttca ccacatcgtc attcaacacc cgcaacgctc cgaattgtac
     1081 tttctccctg tcatgccagt tcccgaagct aaacgaatga tgactatcca gccatccgaa
     1141 gtttacgtgg ccccgccctg cggcgggatg aaatatggtt gtcatagaat ggaaaattcc
     1201 tcagttcgcc agcggatcca ccggcccggt caacactact tcctccaccg gatcggatgg
     1261 cttgtccgcg ttatattcaa cgataaaatt attgcgcccc tcaccgatga ggatcgacat
     1321 atagttcagc tgtaccagtt cgagcatcga caaaaaaatg aagatcgcct gcaacctgtc
     1381 tctacacact tcgaatagcc tctcgaatga cagcgtccgt tgctcacgta cggtcgtgag
     1441 gatatgtttc cggcttcctt ccatcgtata gttgtattgc acgacggtat gcacgggcct
     1501 gttctgacgt tgctggatcc gttgcatcac cttctcgaaa gtcttcatta atttgaaaag
     1561 tgtgatcgcc tggatctcgg tgccctcgcc cgcctcttcg ccgatcccgg acaattcttt
     1621 ttgcagattg cccctgcgca ccatcaggtg acggatcgcc tccatttcgg caagcgtagc
     1681 cgctgcctcc ttgtaacgct tgtactccaa cagcttgtcg atcaattcct gacgcgggtc
     1741 gatctcgttg ccctccgcgt ccagttcctt ccgtggcaac agcattttgg ccttgatacg
     1801 catcagggtg gagatgaaga ggatgaattc gctggacagc tcgatgttca gactctcctg
     1861 cgaatggata taggtgagga aatcctggat gatgttgtgg atcggtatat tgtagatgtc
     1921 caattcgtcg cgttcgataa aaaagagcag caggtcgaac ggaccttcga attgtggcaa
     1981 tttgatttga tagggcgtca tgatccgacg aaagtagtgc tattttgggg aaaaccggac
     2041 ccggttttcc ccaagttgtt aagccccgcg ccatccacgt catcccatgc accatctccg
     2101 gtcccgcacc atccacgtca tcccatgcac catcaccgcc gcacccttcg cggctccgca
     2161 cactactgcg caatcttcac ggcctcggac ccctcccgct ttttcttggc gaacttatac
     2221 gccagcccga ccccgagcac ctcctgcagc tgcagcctgg gtccgccaaa agtcccatcc
     2281 tctttcaccg aatggatcct gtcgtcatag accagcgtca acgccagatt catactgagc
     2341 agcgtcgtca ctttgacatt cagcacattg gtcatataca aggcgatatt ctgcggattg
     2401 ttcagatagt ccgagaacag gtccagcctg cccgtatagc tagccgtttt actcagcggc
     2461 gagttatagt tgatcgacgc ataggcgccg aattcgaacc gggatttttg tcccgaatcc
     2521 accccgaacg ccccatgagc cgcaaggctg tcattgtcga cgatcagctc gcggaccgtc
     2581 gccggcgaaa ggaagagcga aaaattatcg gtcggtttgt aatccatacc gatcgagaac
     2641 aacagatagg ccggagccag aaagtccgaa gtcagcacct ggtgtgtggc atcggggtaa
     2701 ttgtaccccg gtgcgaactg cgtccggaag tcgaacaggc cggtgagata ccacttatgg
     2761 cccagatcat acccatactt tgaggtgata ttgaaccgat cgtccgtttt acgggtacca
     2821 aggctcgtgg tattagcgat gccatatcct accaccagaa aattatccca ggatcgccgg
     2881 ccatccaggt aaaaggcata caggtttacc aacccgttga atgccagggc cgatttatca
     2941 ccgccggccg accagttgct cagcgccgtc tggttgaagg tgagattgat caaacccccg
     3001 gttttccagg tcatcttagt cgtgtctttg ggatccttgg taatagcggc gccgctgact
     3061 gccttctgca gatcgttctg agcctgtagg aaagtcgtga agccaacggt gaaaaggaag
     3121 acaataagcc atctccgcgc acaatgattc gtcatagtcg tcagtttatt tcggtttcaa
     3181 aagctatcaa aaattctgcc agcggcatcc atccaccgcc aaagcgctca caccagcgcc
     3241 atcccgcccc gcatccatcc accgccaaag cgctcatccc accggcatcc cgccccgcat
     3301 ccatccgccg ccagaacgct catcccgccg gcatcccgcc ccatcggcag cgcccttcgg
     3361 cccccttccc taagccgcct tgcccttctt caattcatcc cttatctcca tcaacaactt
     3421 atccgtactc gatggctccg ccggcgccgc ggcttttttc ttatgcagag aatgaatacc
     3481 cttgatgaag aggaagagga taagggcgaa gatgatgaac ttcaggacgg cagacaggaa
     3541 aagcccgtat tttacggctc cccaggccag tttgtcgatg tccgagagat gcgccgcttt
     3601 cagtgctgga gccagcaata gaggagtgat cacattgtcg gtcagcgacg taacgatggc
     3661 ggtgaatgcc gcgccgacga tcacagcaac ggcaagatcg atgacactgc cgcgcataac
     3721 gaagtctctg aactctttta aaaaggccat gacggttggt tttaattatg aagaatctat
     3781 tttttcaatc ccgggtactg catgttcagg tctcttaggg tatccctcaa cgtgtgagcg
     3841 atgagatatt ccttatacca gttttgatcc gcgggtacga tcttccaggg tacttcgctg
     3901 caattttcga aacaatcctc gtaggcctcc atatattcat cccatagttt ggcctctttg
     3961 aaatcgtcct cgttgtattt ccattgcttg gaagggtttc ttatgcgctc ttccaatcga
     4021 tgtttctgtt cgtcgatcga gatgtggaga tagaatttca ggatatgtgt atcattatgc
     4081 ccgatcagca atcgctcgaa atgattaatg g
//
LOCUS       DASYSV010000131         2954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2074076, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000131 DASYSV010000000
VERSION     DASYSV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2074076"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VGQ51_04185"
     CDS             <1..283
                     /locus_tag="VGQ51_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9035788.1"
                     /translation="VENNNNLRYTSGIRMWPNPVQNYLNVWYQSADHDRFVVSVISMS
                     GVNMMTTSYDNVNGQISFHINVGRLARGTYFLVVTDSNGQKTVRQFVKQ"
     gene            complement(316..936)
                     /locus_tag="VGQ51_04190"
     CDS             complement(316..936)
                     /locus_tag="VGQ51_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEV9035789.1"
                     /translation="MSIIRKIKSSQRNKELVLKILMPSGQARPRWWVRSFVNPFVHRR
                     GRHSVIRPNVRLDVVPFRKFVVGRHTIIEDFSCINNGMGDIIIGDHCSIGLSNTLIAP
                     VTIGNNVILAQNICLSGLNHGYEDIHTPIRFQKCTTAQITVEDDCWIGSNSVITAGVT
                     IGRHSVVAAGSVVTRDVPAFSVVGGNPARLLRQYNETSGKWERPPQ"
     gene            complement(933..2117)
                     /locus_tag="VGQ51_04195"
     CDS             complement(933..2117)
                     /locus_tag="VGQ51_04195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9035790.1"
                     /translation="MDIIITGLSPWYFEIGSNCKSIALELSRHHRVLYVNMPLDRRTI
                     RHESSNPDIRRHIGIVRHKQENLLRIGPNLWNYYPYHILESANWLPSTSLFKIFCRIN
                     SRRFAADIHRAAQRLGFKDYVFFNDNDIFRSFYIREFLRPKVYIYYSRDNLLGVPYWK
                     KHGQKIEPLHIAKADIAVANSIYLANYLRQHNPNSHYIGQGCNITLFDPTIPRPVPPD
                     MTTIRGPIIGYVGAILSLRIDIAILLHIAKTCPEWNIVLVGPEDDFFKASPLHDMPNV
                     HFLGRKDINDLPAYVARFDVCINPQLINAVTVGNYPLKVDEYLAMGKPVVATRTETMQ
                     IFENCVYLAEKPEDYPGFIKAALSENDPSFTEKRIKLARTHTWTACVDTLYSLIAKKL
                     KN"
     gene            complement(2175..>2954)
                     /locus_tag="VGQ51_04200"
     CDS             complement(2175..>2954)
                     /locus_tag="VGQ51_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035791.1"
                     /translation="TQPRDDANPTQRQILLAGKDSIMAEFPGRALDFWTPVTDPSTLG
                     ILPQYSKGDAIHLNDAGHAVIAQVAENTNIMDPVPLALTLTAFTARWTGQNVLLQWTG
                     VNDDPSDPILFDVQRSTNGNAFSSLYKMTTTVSGSWSYTDNDCPAGIAYYRLSWLDGA
                     KTSYSKTIPINHSTGKLSIDNLYLSGGSQLVTDMELPAPGDASIAILSLSGRLILRKA
                     YSGLPQSGTLSIPLPQLAGGEYVLKIMTSNGQTTAKPFVKF"
BASE COUNT          616 a          656 c          948 g          734 t
ORIGIN      
        1 tgtagagaat aataataacc tgcggtatac cagcggtatc cggatgtggc cgaaccctgt
       61 gcagaactat cttaatgttt ggtatcagtc cgcggatcac gaccggtttg tagtgtctgt
      121 catttcgatg agtggagtaa atatgatgac gacgtcttat gacaatgtaa acgggcagat
      181 cagctttcac atcaatgtcg gccggctggc gcgcgggacc tacttcctgg tggtgaccga
      241 cagcaacggg cagaagacgg tcaggcagtt tgttaagcaa taaagaaatg agataatttt
      301 tcgaggccgc tccatttatt ggggcggcct ttcccattta ccggatgtct cattatattg
      361 gcgaagaagg cgggccgggt tgccgccgac cacgctaaat gccgggacgt cgcgggtgac
      421 tacgctgccg gcagcgacaa cggagtggcg cccgatggtg acacccgcgg taatgacgct
      481 gttcgatcct atccagcagt catcctccac cgtgatctgg gccgtggtgc atttttggaa
      541 gcggatgggc gtatggatat cctcgtatcc atggttgaga cccgagaggc agatgttttg
      601 cgcgagaatg acgttgttgc cgatggtcac cggggctatc agggtattgc tgagaccgat
      661 gctgcagtga tccccgatga tgatgtcgcc catcccgtta ttgatgcagg aaaaatcctc
      721 gatgatcgta tgtctgccca cgacgaattt tcggaagggc accacatcca gccttacatt
      781 gggtctgatg accgagtgtc ttccccgccg gtgtacgaat ggattgacga aggaccttac
      841 ccaccagcgc ggtctggcct gtccggatgg catcaggatc ttcaggacca attccttgtt
      901 ccgctgactc gattttattt tccggatgat gctcaatttt tcagtttttt agcgatcagg
      961 gaatagagtg tatctacgca ggcggtccag gtatgcgtgc gtgcgagttt gatccttttt
     1021 tcggtgaatg aagggtcgtt ttccgacagg gccgctttta tgaaaccggg atagtcttcc
     1081 ggcttttccg ccaggtatac gcaattttcg aagatctgca tggtctccgt ccgggtggcg
     1141 acgacaggtt tgcccatggc caggtattcg tcaactttaa ggggataatt gccaacggtg
     1201 accgcgttga tcaattgggg attgatgcaa acatcgaaac gggccacgta ggcgggcaga
     1261 tcgttgatgt ccttccttcc caggaaatgg acgtttggca tgtcatggag tgggcttgcc
     1321 ttaaagaaat cgtcttccgg gccgaccagc acgatattcc attcgggaca tgtctttgcg
     1381 atatgcagga ggatcgcgat atctatgcgg aggctgagta ttgcgccgac atacccgatg
     1441 atgggtcccc ggatcgtcgt catatcgggc ggaacgggcc gtgggatcgt gggatcgaag
     1501 agcgtgatat tgcatccctg accgatataa tggctattgg gattgtgttg acgaaggtaa
     1561 ttggccaggt agatggaatt ggccactgct atatctgctt ttgcgatgtg tagcggttcg
     1621 atcttctgtc cgtgtttttt ccagtaggga acacccagca ggttatccct gctatagtag
     1681 atgtagactt ttgggcgaag gaattccctg atgtaaaacg accggaagat atcgttgtcg
     1741 ttgaagaaga cgtagtcttt gaatcccagg cgttgggccg ccctgtggat gtctgccgca
     1801 aatctccggc tgttgatccg gcaaaatatt ttgaacagcg aggtcgaggg gagccagttg
     1861 gcggattcaa ggatatggta cgggtaatag ttccaaaggt tgggtccgat ccggagtaga
     1921 ttttcctgtt tgtggcggac gatcccgatg tgccggcgga tatcggggtt ggacgattcg
     1981 tgtcggatcg tccggcggtc gagcggcatg ttaacataca ggacccggtg gtgacgcgac
     2041 aattccaggg caatgctctt gcagttactg ccaatctcga aataccatgg tgagagtccg
     2101 gtgatgatta tatccatagc cagcggagaa aggtcttcta tgcggccgct aaaatagaaa
     2161 gaaagtgcga gattttagaa tttgacgaat ggtttggcgg tcgtctgtcc gttggatgtc
     2221 atgatcttca ggacgtattc accgccggcc agctggggca gggggatgga gagggtgccg
     2281 gattgcggga gaccggagta tgcctttctt aggatgaggc ggcccgagag gctcaggatc
     2341 gcgatcgaag cgtctccggg tgcgggaagt tccatatccg tgacgagctg tgaaccgccc
     2401 gataaataga gattgtcgat ggatagtttg ccggtagaat ggttgatcgg gatggtcttc
     2461 gaatagctgg ttttggcgcc gtcgagccag ctcaggcggt agtaggcgat accggccggg
     2521 cagtcgttgt cggtgtagga ccaggagccg gagacggtcg tggtcatttt gtacagtgat
     2581 gagaaggcat tgccgttggt gctgcgttga acgtcgaaaa ggatggggtc ggagggatcg
     2641 tcgttgaccc cggtccattg gaggagtacg ttctggcctg tccatcgcgc ggtgaaggcg
     2701 gtaagggtaa gggcgagggg tacagggtcc atgatattgg tgttctcggc cacctgggcg
     2761 atcactgcat ggccggcatc gttgagatgg atggcgtcgc ccttgctgta ttgcgggagg
     2821 ataccgaggg tggaagggtc ggtaacgggg gtccagaaat cgagggcgcg gccggggaat
     2881 tccgccatga tggagtcttt acctgcgagg aggatctggc gttgcgtggg gttggcgtcg
     2941 tcgcggggct gggt
//
LOCUS       DASYSV010000132         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2331107, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000132 DASYSV010000000
VERSION     DASYSV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331107"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VGQ51_04205"
     CDS             complement(<1..223)
                     /locus_tag="VGQ51_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035792.1"
                     /translation="MRLSLLFPEEGVTAPDRRRIIRGTLHSFLIQGFSIALVFISNWW
                     IVRLSDPDDYGRYVHVFNWVSILSVIVLGG"
     gene            complement(220..981)
                     /gene="tpiA"
                     /locus_tag="VGQ51_04210"
     CDS             complement(220..981)
                     /gene="tpiA"
                     /locus_tag="VGQ51_04210"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV9035793.1"
                     /translation="MRQQIAAANWKMNLTLSQGEQLLDGILGADIALAPHQLAVFAIP
                     FPYLAMAVGKLKGRKNFFVSAQNCSDEKAGAYTGEVSAEMLRSINVGYCVLGHSERRE
                     YFSERNQSLAKKVELCLENNIIPIFCCGEELSIREVGAQNGFVETQLMESLFHFSEEQ
                     IRKIVIAYEPIWAIGTGKTASVEQAQEMHAHIRSLFAKKYNEATADHLPILYGGSVKA
                     ANSKELFHSPDVDGGLVGGASLNAAEFTSIIRSLK"
     gene            complement(978..>1735)
                     /locus_tag="VGQ51_04215"
     CDS             complement(978..>1735)
                     /locus_tag="VGQ51_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEV9035794.1"
                     /translation="EALQEDFDLLTTSRVSEPIPPSVQAINPEMIFIETGARLQHCML
                     NASTGPIYIARDAEIMEGSLIRGPFAACESAVVKMGTKIYGATTLGPGAVVGGELKNV
                     VIFGYSNKGHDGYLGDSVIGEWCNLGAGTSVSNLKNNAGEVRVWHPASRDFLRAGLKC
                     GLLMGDYSRAAINTSFNTGTVVGVCCNIFGDGLPPKYVADFSWGIEGATRYEWEKALR
                     DIGNWKNLKNRSLTEPEIETLKHIFEQHKTLHS"
BASE COUNT          370 a          493 c          476 g          396 t
ORIGIN      
        1 gtcctccgag tacgatcacg ctcaggatgc tgacccagtt gaatacatgg acataccggc
       61 cgtaatcgtc gggatcggac agccggacga tccaccagtt ggatatgaac accagggcga
      121 tggaaaagcc ctggatcaga aaggaatgca gggtcccccg gatgatgcgc cgccggtccg
      181 gcgccgtcac gccttcttcc gggaaaagga ggctcaacct catttaagac tgcggatgat
      241 ggacgtgaat tcagccgcgt tcagcgaggc tccgcccacg agaccgccgt cgacatcggg
      301 agaatgaaaa agctctttgg aattggccgc ctttacgctt ccgccataga ggatgggcag
      361 gtgatcggcg gtcgcctcgt tatatttttt tgcgaaaagg ctccggatat gggcatgcat
      421 ctcctgcgcc tgttccaccg aagcggtctt gccggtaccg atggcccaga tgggttcata
      481 ggcgatgacg atctttcgga tctgttcttc ggagaaatgg aacagcgact ccatcagctg
      541 cgtctcgaca aacccgttct gcgctcctac ctcgcggatg ctcaattcct cgccacagca
      601 aaatatcggg atgatattat tttccaggca gagctcgacc tttttggcca gcgattgatt
      661 acgctcggag aaatattccc ttctttcgct atgcccgagg acgcaatacc ctacgttgat
      721 ggaacgcagc atttctgccg acacttcccc ggtataggcg ccggctttct catcggagca
      781 gttctgcgcg gagacgaaaa agttcttccg gcctttcagt ttcccgacgg ccatcgccag
      841 gtaagggaat ggaatggcaa acacggccag ctgatgtggg gccagcgcga tatccgctcc
      901 aaggatgcca tccagcagct gctctccctg ggaaagcgtc aggttcattt tccagttggc
      961 ggccgcgatt tgttgtctca tgaatgaagg gttttgtgct gttcaaaaat atgttttagt
     1021 gtttcgatct cgggttcggt gagcgatcgg ttcttcaggt ttttccagtt gccgatgtcg
     1081 cgaagcgcct tctcccattc gtacctcgtc gctccctcga tcccccagct gaaatcggca
     1141 acatatttcg gcggcaggcc gtcgccgaag atattgcagc agacaccgac gaccgtaccg
     1201 gtattgaaag aggtattgat agccgcgcgg ctatagtccc ccatcaacag accgcattta
     1261 agaccggcgc gaagaaaatc ccgcgatgcc gggtgccata ccctgacctc gccggcattg
     1321 ttcttcagat tggagaccga agtgccggcg ccgagattgc accactcgcc gatcaccgag
     1381 tcaccgaggt agccgtcgtg acctttattg gaatagccga agatcacgac attcttgagc
     1441 tcgcctccaa cgaccgcacc ggggccgagc gtggttgcac cgtatatctt cgttcccatt
     1501 ttaacgacag cactttcgca ggctgcgaat gggccccgga tcaacgagcc ttccatgatc
     1561 tcggcgtcgc gggcgatata gatcggaccc gttgaagcgt tgagcataca atgttgtagt
     1621 ctggcgcccg tctcgataaa gatcatttca ggattgatgg cctggacgga cggcggtatc
     1681 ggctctgaga ccctgctcgt ggtcaacaga tcgaaatctt cctgcagcgc ttcag
//
LOCUS       DASYSV010000133         4412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1178845, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000133 DASYSV010000000
VERSION     DASYSV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178845"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VGQ51_04220"
     CDS             <1..255
                     /locus_tag="VGQ51_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035795.1"
                     /translation="DFDLKRYFLSIIKIICYYEYEPRLMAFGIVERLAPEISNATRAK
                     ALKFLEAHRIRLERDAIEKGENSSLHFVEKTQELLSQHHL"
     gene            complement(456..1487)
                     /locus_tag="VGQ51_04225"
     CDS             complement(456..1487)
                     /locus_tag="VGQ51_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035796.1"
                     /translation="MNYRTPLLALLIAVTFTRCGSHDERPDISGISVGDIHIDRFDTA
                     FFGLDSNHIAAGLYRLDHEYPWFTGAFVSDILGAGPLTDSNHVAFEAARQFLVSYLPI
                     RDSLEPKYRRLDWLERQLQTGFRYVKYYFPRYKLPPKVVAFIGPFDGPGVALTTYSLA
                     IGLQSYAGRNFSLYLTPKGQDMYPQYISRRFEPEYIAANCLSAIAGDIFPDSSDGRPL
                     IEEMVTKGRYWWLAGRLMPDTPDSIYTGYTKAQLDWCTVNESQIWNFFLQSNDLLYTV
                     DPDQIKNYIGEGPKTLAMPDGSPGNIGAWVGWQIVKKYVSDHHGLTPLQLMQTPVRTI
                     FEESKYKPR"
     gene            complement(1484..1837)
                     /locus_tag="VGQ51_04230"
     CDS             complement(1484..1837)
                     /locus_tag="VGQ51_04230"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEV9035797.1"
                     /translation="MAFNEQLAHRIRESLAHLPDVEEKKMFHGICFMVNGKMCLCVRL
                     DEMLCRIGPHKYTEALDMSHCRPMIHNGRTMTGFVFVAGEGIKRKKDFEYWVNLALDF
                     NKEAKASKKSLKKKK"
     gene            1864..3768
                     /gene="nadE"
                     /locus_tag="VGQ51_04235"
     CDS             1864..3768
                     /gene="nadE"
                     /locus_tag="VGQ51_04235"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEV9035798.1"
                     /translation="MKIALAQQNYHIGNFEENTRKILAGIAHAKSVGADLVVFSEMSV
                     CGYPPRDFLEFDDFIVQSYAAVDRIRQEADTIGVIIGAPQRNPAKEGKELFNSVLLLH
                     EKEIKGVAHKTCLPNYDVFDDYRYFEPAYEWKVIPFKGKKIALTVCEDIWNLGDNPLY
                     RDCPMDHLIRQHPDVMINISASPFDYDHDDDRKEVVRQNVLKYHLPMYYCNAVGAQTE
                     VVFDGGSVIYDAAGNLLKELRYFEEDFACVPLSHPVPPLYEELLPGLSVAINNPDPED
                     DLEQVRATSSTVTRRPADPATVFDKDMRVSKQNDPERVLAYLTDERNIRQIRQALILG
                     IRDYFSKMGFSKAIVASSGGIDSAVTLALACEALGAGNVRAVLLPSAYSSGHSVSDAE
                     QLSRNLGNQYDIIPIREVYETMLKALQPAFGDLPKADGDGAPANERSEGCEREGARDE
                     APPKLQSLAVGLAEENLQSRIRGNLVMALANKFGYILLNTSNKSELATGYGTLYGDMA
                     GGLSVIGDVYKLQVYALARDINREGEIIPLNILEKAPSAELRPGQKDSDSLPEYEVLD
                     KVLYQYIERRQGPKEIIALGIDEALVRRVLRLVNISEYKRNQFCPIIRVSAKAFGVGR
                     RVPIVGKYLA"
     gene            complement(3779..4195)
                     /locus_tag="VGQ51_04240"
     CDS             complement(3779..4195)
                     /locus_tag="VGQ51_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035799.1"
                     /translation="MFSKWTILAVTAGILSGCLRSNSSGKDVEVLTGSPWKYEKAGFD
                     NDDGTFDALDPQIGDRDNTIIFCKDGTGYSRIARRAVGMPDTLPFMWSFQNGDSTIYF
                     QNQYYRVKLLTRQRLEIYADQRYGGTSTRYTIILKR"
BASE COUNT         1051 a          976 c         1326 g         1059 t
ORIGIN      
        1 gactttgatt taaaaagata ttttctttcc attattaaga taatttgtta ctatgaatat
       61 gaaccaaggc ttatggcatt tgggattgta gaaaggcttg ccccggaaat ttctaatgcg
      121 actagagcaa aagcgctaaa attcctggaa gctcaccgaa taaggcttga gagggacgca
      181 attgaaaaag gggaaaatag ctcgctgcat ttcgttgaaa agacgcaaga attgttatct
      241 cagcatcatt tataaaaggt tatagcattc tacagtatta gcctggtagg agtcaacagt
      301 ttgatatcgc aatatgcgat atcatgttgg gacgtgacgg cggctggggt tgcgcggcat
      361 ggcggcatgc gacggcgcgg cgggcgggat gacgcgacag gcgagatcgc tgcggaggcg
      421 ggatgacgcg acctacggcg gcgcggcggt cggctctacc gcggtttgta tttcgactct
      481 tcgaagatgg tccgcacggg cgtctgcatg agctgcaatg gtgtcagacc gtgatgatcc
      541 gacacatatt tctttacgat ctgccatccg acccaggcac cgatatttcc gggcgatccg
      601 tcgggcatgg cgagggtctt ggggccttcg ccgatgtaat tcttgatctg atcgggatcg
      661 acggtataga ggagatcgtt cgattgcagg aaaaaattcc agatctgcga ttcgttgacg
      721 gtacaccagt cgagctgcgc ctttgtataa ccggtataga tactgtcggg ggtgtcgggc
      781 atcagccttc ccgccagcca ccagtagcga cccttagtga ccatttcttc tatgagcggc
      841 cggccgtcgc tgctgtcggg aaagatatct ccggcgatgg cgctgagaca attggccgcg
      901 atgtattcgg gctcaaaacg cctggagata tactgcggat acatgtcctg ccccttgggc
      961 gtcaggtaga ggctgaaatt cctgcccgca taggattgga gaccgatcgc gagcgaataa
     1021 gtggtgagcg ctacgccggg gccgtcgaac ggaccaataa acgccacgac cttgggggga
     1081 agcttgtacc ggggaaaata atattttaca taccgaaatc cggtttgcag ctggcgctcc
     1141 agccaatcga gacgacggta tttgggttcg agcgaatctc tgatgggcaa atagctgacc
     1201 aggaattgcc gggctgcttc gaaggcgaca tgattgctgt cggtgagtgg acctgcacca
     1261 aggatgtcac tgacgaaggc gccggtgaac cagggatact cgtgatcgag ccggtatagg
     1321 ccggcggcga tatgattcga gtccagacca aagaaggcgg tatcgaaccg gtcgatatgg
     1381 atgtctccga cgctgatgcc ggagatgtcg ggtctttcgt cgtgactgcc gcagcgggta
     1441 aaggtgactg ctatgaggag ggccaaaaga ggggtacgat agttcatttc ttttttttaa
     1501 gcgatttttt cgaggccttc gcctctttat tgaaatccag ggcaagattg acccagtatt
     1561 cgaaatcttt ttttcttttg atgccttcgc cggcgacgaa gacgaagcct gtcatcgtcc
     1621 ggccattgtg gatcatgggc cggcaatggc tcatatccag cgcctctgta tatttatgcg
     1681 gcccgatgcg acagagcatc tcatccagcc ggacacaaag acacatcttg ccgttgacca
     1741 tgaaacagat gccatggaac atcttttttt cttctacatc gggcagatgg gcgagcgatt
     1801 cccgtatccg gtgggctagt tgttcgttga aagccatgtt caaatatagc ttaaatttgt
     1861 cctatgaaaa tcgcactcgc gcagcagaat tatcatatcg ggaattttga agagaatacc
     1921 cgaaaaatat tggcaggtat cgctcatgcg aaaagcgtcg gcgccgacct ggtcgtcttt
     1981 tccgagatgt ccgtttgcgg ctatccaccg cgtgacttcc tggagttcga cgattttatc
     2041 gttcagtcct atgccgcggt ggatcgtatc cggcaggagg ccgataccat tggtgtaatt
     2101 atcggtgcgc cgcagcgcaa tcccgcaaag gaaggcaagg agcttttcaa ctccgtgtta
     2161 ttgttgcatg aaaaagagat caagggggtt gcgcataaga cctgtctgcc caattatgac
     2221 gtcttcgacg attaccggta tttcgagccg gcctatgaat ggaaagtgat ccctttcaaa
     2281 gggaagaaga tagcgctgac ggtatgcgag gacatctgga acctcgggga caacccgttg
     2341 taccgggatt gcccgatgga tcacctgata cggcagcatc ccgatgtgat gatcaatatt
     2401 tcggcatcgc ccttcgacta tgatcacgat gacgaccgca aggaggttgt aagacagaac
     2461 gtgctgaaat atcatcttcc catgtattat tgcaatgcgg tgggtgcaca gacggaggtc
     2521 gttttcgacg gaggcagcgt gatctatgat gctgcgggca atctgctcaa agaactgcgt
     2581 tattttgaag aagattttgc gtgcgtgccg ttgtcgcatc cggtgcctcc gttgtatgag
     2641 gagctattgc cgggattatc ggtggcgatc aacaatcccg atcccgaaga tgatcttgag
     2701 caggtgaggg cgacgagttc cacagtgaca cggcggcctg ctgatccggc gaccgtcttc
     2761 gataaggaca tgcgggtctc gaaacaaaac gatccggaga gggtgttggc ctatctcacc
     2821 gatgagcgga atatccgtca gataagacag gcattgatcc tggggatccg cgactatttt
     2881 tcaaagatgg ggttttcgaa ggccatcgtc gcgagcagcg gcggtatcga tagcgccgtg
     2941 acgctggcgc tagcgtgtga agcgttgggg gctggcaatg tgcgggctgt attgttgcca
     3001 tcggcttatt ccagcgggca ttcggtcagc gatgccgaac agttgtcgag gaatctggga
     3061 aatcagtatg atattattcc tattcgggaa gtctatgaaa cgatgctgaa ggcgctgcag
     3121 ccggcgtttg gggatctacc aaaagccgac ggagacggcg cgcccgcaaa cgagcgaagc
     3181 gaaggttgcg agcgagaagg agcgagggac gaagctccgc cgaagctcca atcattagcg
     3241 gtcggtttgg cggaggagaa tctgcagagc agaattcgcg gaaacctcgt gatggcgctt
     3301 gcgaataagt tcggatatat actattgaat acttccaaca agagcgagct ggcaacgggg
     3361 tatgggacgt tgtatgggga tatggccggg ggtttgagtg tgatcggcga tgtatataag
     3421 ttgcaggtat atgcgctggc gcgggacatc aatcgggaag gagagatcat tcctttgaat
     3481 attttggaga aggcgccatc ggccgagttg cggccgggtc agaaggatag tgatagtctg
     3541 ccggagtatg aagtgctcga taaggtgctt tatcaatata tcgagcgccg ccaggggccg
     3601 aaagagatca ttgcattggg tatcgatgag gcactggtgc ggcgggtgtt gagactggtg
     3661 aatatcagtg agtataagcg aaatcaattt tgtccgatca taagggtcag tgcgaaggcg
     3721 tttggggtag ggcgaagggt gccgatcgtg ggaaagtatc tggcgtgatc gtcgcgtact
     3781 agcgtttgag gatgatcgta tagcgggtgc ttgtgccgcc gtaacgctgg tcggcgtaga
     3841 tctcgagacg ttgccgggtg aggagtttta cgcgatagta ttgattctgg aaatagatgg
     3901 tgctatcgcc attctgaaag gaccacatga agggaagcgt gtcgggcatt ccgactgcgc
     3961 ggcgggctat ccgggaatag ccggtaccgt ctttacaaaa gatgatggta ttatcacggt
     4021 ccccgatttg agggtcgagg gcgtcgaagg tgccgtcgtc gttgtcgaat cctgcttttt
     4081 catatttcca cggacttccg gtgagcactt ccacgtcttt tccggaagaa ttcgagcgta
     4141 agcatccgct aaggataccc gcggtaacgg ccagtatcgt ccatttagaa aacatcaggc
     4201 gcatcatttt cgtttagaag gatagggtgt ccctcaggtt taaaactccc gggacggcaa
     4261 cttattgtta acatatgaaa ttaaaattat attagttatc cacataaggg ccgggaaagg
     4321 aagatgccaa aattgttaaa atttggcagg ctagttgccg gtagtgatcg acgaaaaagg
     4381 cagatggctg atggtcagcg agcgatacgg at
//
LOCUS       DASYSV010000134         7100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_26108, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000134 DASYSV010000000
VERSION     DASYSV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26108"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VGQ51_04245"
     CDS             complement(<1..1501)
                     /locus_tag="VGQ51_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV9035800.1"
                     /translation="MIPETLDFNKNEDSMRRAISQLKTRLDKIALGGGKAAIEKQHEK
                     KKLTARERIRYLLDKDTPFIELGALAGFELYEEQGGCPAGGTVAGIGYVSNRQCVILA
                     NDQTVKAGAWFPITGKKNLRLQEIAMENHLPVIYIVDSAGVFLPMQDEIFPDKEHFGR
                     IFRNNARMSAMGITQIAAVMGPCVAGGAYLPIMSDETLMVEGNGSIFLAGPYLVKAAI
                     GEDIDAETLGGAVTHTEISGIADYKFKTEQECLDQVRNIVGRLGHPAGNGIFDRTTPA
                     PPRHPAEELYGILPADASKPYDMTDIISRIVDNGVFEQFKEDYGKTILCGYARIDGWA
                     VGIVANQRRIVKNRKGEMQMGGVIYNDSADKAARFIMNCNQKKIPLVFLQDVTGFMVG
                     SRSEHAGIIKDGAKMVNAVANSVVPKITIIIGNSYGAGNYAMCGKAYDPRFIYAWPTA
                     RIAVMGGEQAAKTLLQIQVSAAKAKGKQIDPAEEKRLLDEIRQRYDSQTS"
     gene            1593..2666
                     /locus_tag="VGQ51_04250"
     CDS             1593..2666
                     /locus_tag="VGQ51_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HEV9035801.1"
                     /translation="MVLIMLAASIKILSFYPTVVERYYSKGVYPELARLQRILFGWIP
                     FSIGDVLYLAAIVLLIWRLVRLIRVLIRRKAEKGWFLLFLRRTLFVVLWVYVLFNGLW
                     GLNYDRPGIADELHLQVRPYSTADLNHLILLLIGEMNRLDSAGRLDRAGFSHMRTLRN
                     GAVAAYDALSGQDPRFTYSRPSVKPSLFSYPGAYIGFAGYYNPFSGEAQVNTRNPLFT
                     QPYTACHEVSHQLGYAKENEANFVGFLAASRSNDPAFRYSVYFDLFLYAVREVAVRDS
                     ALIRSYRDSLGPGVREDLRELQRFNRKYANPMEPVIWGLYGKYLRANRQPHGITTYTE
                     VTAWLIAYIRQYGEGALEGKGPR"
     gene            complement(2689..3225)
                     /gene="dcd"
                     /locus_tag="VGQ51_04255"
     CDS             complement(2689..3225)
                     /gene="dcd"
                     /locus_tag="VGQ51_04255"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEV9035802.1"
                     /translation="MILSDTRILQEIEKGTIKIDPYDRGCLGSNSYDVHLGKWLATYR
                     EHILDARKHNEIEYFEIPEEGFVLYPHIFYLGVTHEYTETHAHVPFLEGKSSTGRLGI
                     DIHATAGKGDVGFCGNWTLEISVKQPVKVYKGMPVGQLIYFPVDGEIMVKYNQKKNAK
                     YNGQPDKPIESMMWKNTF"
     gene            3309..3974
                     /locus_tag="VGQ51_04260"
     CDS             3309..3974
                     /locus_tag="VGQ51_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEV9035803.1"
                     /translation="MPLFYQHNINQETKLGIWHIAEAESFFLEKVPLKRGVSHPFKRL
                     QHLAGRYLLVTLFPDFPLDEIRIADTRQPFLASEKYHFSISHCGNYAAAIVSKNSRVG
                     IDIELITPRIERLADKFLHEEEAHFFNDDYALFLEQWGLRGRVFQEFLTLIWNAKEAI
                     YKWYGRGELDFKRHMRLEGGINPDGDWIKLPFIFAKGKCIHLGVEGRIFEERSLTLAW
                     VLT"
     gene            4095..5135
                     /locus_tag="VGQ51_04265"
     CDS             4095..5135
                     /locus_tag="VGQ51_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HEV9035804.1"
                     /translation="MKNLFLFAGMLFFLCSCSSTNLMSLSVTVPAPVSLSPAAHSAAV
                     VNRSGDDNSAVDRLHRTLSLETRNLQTEGARASMRGLADELIKDGRFANVIRLDSLDL
                     RSYGAGVFPTYLSWDTVERICRQNHADVLFSLELFDAASKVNVGSAPALLANPLGAVA
                     VLDQPFGVNTRVKTGWRIYDPATRNILDEYVLYKDLDFQGRVGNPVRAANSMLGRQEA
                     VRQAGIEAGQSYALRIVPYSIRVSRFYFVRGNGNFIIAKRMAQSGDWDGAARIWQQES
                     TSSSRKVAGRACYNMAIIREINGDLNGAVTWSTKAYEVYNVRLALSYLSVLHNRQNED
                     AVLKSQTELTGN"
     gene            complement(5177..6376)
                     /locus_tag="VGQ51_04270"
     CDS             complement(5177..6376)
                     /locus_tag="VGQ51_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV9035805.1"
                     /translation="MPTEIKCPNCGHQFPMEDAVAEEYKKDLREQMQKFIRDKEASFV
                     RKLEEEKSNLQTTLEKSLRNSIGSDYENKLRLLEDARKDTEEKLRVSRQREFEFLKKE
                     QELQTKEAELELAVQRLLQEERVKLSKELRDLEEQKIAAKETEFRLRLKELEKQLDDQ
                     RKLAEEMRRKAEQGSMQLQGESQELALEELLRSAFPFDKVSEVGKGVRGADCIQTVFN
                     KSGLECGRIIYESKRTQSFSQEWVEKLRADMRGQGAVVAVIVTQTMPKDLERFGEING
                     VWVCSFKEVKALAHVLRDGIIRIFNITKSQENKGDKMTLLYNYLTSAEFSEQWKAIRE
                     GFLSMKQSIQKERDTMERLWKAREKQLEKVLLNAAHVRGSIEGIAGKDVIDVPLLEDQ
                     DDDLLTE"
     gene            6411..6896
                     /locus_tag="VGQ51_04275"
     CDS             6411..6896
                     /locus_tag="VGQ51_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035806.1"
                     /translation="MVELTYDEILEVKASLQSYVDKVRMEIEIIEGALQLLTKISNNK
                     KINVSTIFLPEEILRLNEVKANKKPGIEWKKAVYETIRKSNFFLSSKMIYDRMLVYHP
                     FQLGDKRYSQKCISSALVNLVADGKVGKFKDESGLYFFGDTERHFEATGKPNLQFFTN
                     T"
BASE COUNT         1539 a         1689 c         1925 g         1947 t
ORIGIN      
        1 gggaggtctg gctgtcgtat cgttgccgga tctcgtcgag caggcgtttt tcttcggccg
       61 gatcgatctg ttttcccttt gctttggcgg cgctgacctg tatctgaaga agggtcttcg
      121 cggcctgttc accacccatg acggcgatgc gggctgtcgg ccaggcatag atgaagcggg
      181 ggtcataggc cttgccgcac attgcatagt tgcccgcacc ataggaattg ccgataatga
      241 tcgtgatctt cgggacgacg gaattggcga ccgcgttgac cattttggca ccgtccttga
      301 tgatccctgc atgttcgctt cgggaaccca ccataaagcc ggtgacgtcc tgcagaaaga
      361 cgaggggaat ctttttctgg ttgcagttca tgatgaagcg ggccgccttg tcggcgctgt
      421 cgttatagat gacgccgccc atctgcattt cacccttccg gttcttgacg atcctgcgtt
      481 ggttggcgac gatgccgacc gcccagccat cgatacgggc ataaccgcat aggatggtct
      541 tgccgtagtc ctctttgaat tgttcgaaga cgccattgtc gacgatgcgg gaaatgatat
      601 cggtcatatc gtagggtttg gacgcatcgg cagggaggat accatagagc tcttcagcgg
      661 gatgtcgcgg aggagccgga gtcgtccggt cgaaaatgcc gttgcccgcg ggatgcccca
      721 gcctgccgac gatgttcctc acctgatcga gacactcttg ttccgtcttg aatttatagt
      781 cggcgatgcc ggatatttcg gtatgggtaa cggcgccgcc cagtgtttcc gcgtcgatat
      841 cttcgccgat ggctgcctta acgagataag ggcctgccag gaagatggat ccgttgcctt
      901 cgaccataag ggtttcgtcg ctcatgatcg ggagataggc gccgcccgcg acacagggtc
      961 ccataacggc agctatctgg gtgatgccca ttgcgctcat tcgggcattg ttgcggaaga
     1021 tgcgcccgaa atgttcttta tcgggaaata tttcgtcctg catgggcagg aagacgccgg
     1081 cgctatcgac gatatagatc accggcagat ggttttccat cgcgatctcc tggagacgga
     1141 ggttcttttt acctgtgatc ggaaaccagg cacctgcttt tacggtttgg tcgttggcga
     1201 ggatgacgca ttggcggttg ctgacatagc cgatgccggc aacggtgccg ccggcgggac
     1261 agccgccctg ttcttcatag agctcgaatc cagcgagagc gccgagctcg atgaatgggg
     1321 tatccttatc caggagatac cggatgcgct cacgcgccgt cagctttttt ttctcgtgtt
     1381 gcttttctat cgccgctttt ccacctccca gcgcgatctt gtccagccgg gtcttcagct
     1441 ggctgatcgc acggcgcata gagtcttcgt ttttgttgaa atccagggtc tccgggatca
     1501 tagtcaacta ttatggatgc aatttaacat ggatgcagaa atattttgga ctatttttag
     1561 ccgctatgag gagacaacgc aaaataacct ggatggttct gatcatgctt gcggcgtcga
     1621 tcaagatcct gtccttttat ccgaccgtcg tggaacgcta ttattcgaaa ggggtctatc
     1681 cggaattggc gcggctgcag cgcatacttt tcggctggat ccctttcagc atcggagatg
     1741 tcctttacct ggccgcgatc gtcctgttga tctggcggct ggttcggctg atcagggtcc
     1801 tgatccgtcg taaggctgag aaaggttggt tcctgctttt tttgcggcgg accttgttcg
     1861 tcgtcttatg ggtttatgtt cttttcaacg ggctttgggg attgaattat gatcggccgg
     1921 gcattgccga tgagctgcac ctgcaggtgc gaccctattc cacggctgac ctgaatcacc
     1981 tgatcctcct gctcatcggt gagatgaaca ggctggatag tgcgggaaga ctggatcgtg
     2041 ccggcttttc gcatatgcgg acgttgcgga atggcgccgt tgccgcgtat gatgcgcttt
     2101 ccggtcagga cccgaggttt acttacagca gaccttccgt aaaaccatct cttttcagct
     2161 atcccggcgc ttatatcggg tttgcgggat actacaatcc tttttcaggt gaggcgcagg
     2221 tgaacacgcg gaatccgtta ttcacccaac cttataccgc ctgtcacgag gtgagtcacc
     2281 agctgggata tgcgaaggag aatgaagcta atttcgttgg attcctggcc gcaagccgct
     2341 cgaatgaccc tgccttccgt tattccgtgt acttcgatct tttcctctat gcggtgaggg
     2401 aggtggccgt tcgggattcg gcgttgatac ggtcgtacag ggatagtctt ggacccggcg
     2461 tgcgggagga cctgcgggaa ttgcagcggt tcaaccggaa atatgccaac ccgatggagc
     2521 cggtaatatg ggggctctat ggaaaatatc tgcgtgccaa ccgacagccc catgggataa
     2581 cgacctatac ggaagtcact gcgtggctga tcgcgtatat ccgccaatat ggtgaggggg
     2641 cgctggaggg gaaaggtccg cggtaggcgc atggatggcc gcggccagct aaaatgtatt
     2701 cttccacatc atactttcga tcggcttgtc gggctgaccg ttgtatttgg catttttttt
     2761 ctggttgtat ttaaccatga tctcgccatc gacggggaaa tagatcagtt gtcctacagg
     2821 cataccctta tataccttca cgggttgttt gaccgagatc tcgagtgtcc agttgccgca
     2881 gaagccgaca tcgcctttac ctgcggtggc atggatgtcg atgcccagcc gcccggtgga
     2941 ggatttccct tcaagaaagg gaacatgggc atgggtttcg gtatattcat gggtcactcc
     3001 caggtaaaaa atgtgtgggt ataacacgaa gccttcctcg gggatctcga aatattcgat
     3061 ctcgttatgt ttacgggcgt ccaggatatg ttcccggtaa gtagccagcc actttccaag
     3121 gtggacatcg taggaattgc ttcccaggca gccgcggtca taaggatcga tcttaatagt
     3181 tcctttttct atttcctgca atatgcgagt atcggatagg atcatattga aaattggtta
     3241 agtttgtgaa ttggcccgtt gaagaagacc gtaggatcat ttgcgaatat gtgaatataa
     3301 ttttagagat gcccctgttt tatcaacata atattaacca ggaaacaaag ttagggattt
     3361 ggcatatagc ggaggcggag tcctttttcc tggagaaggt acctttgaaa aggggtgtct
     3421 cgcatccgtt caagcgtctt cagcacctgg caggccgata tctgctggta accctgttcc
     3481 ccgatttccc gttggacgag atacggatcg cggatacccg gcagccattc ctggcgagtg
     3541 agaaatatca cttttccatc tcgcattgcg ggaactatgc cgccgccatc gtcagcaaaa
     3601 atagtagggt ggggatcgat atcgagctga tcacgccgcg tatcgagcgc ctggcggata
     3661 agttcctgca tgaagaggag gcgcactttt tcaatgacga ctatgcgctt tttctcgagc
     3721 aatggggatt acggggacgg gtattccagg aattcctgac gttgatctgg aatgctaaag
     3781 aagcgatcta caaatggtat gggcgtgggg agctggattt caagaggcat atgaggctgg
     3841 aggggggcat taatccggat ggggattgga tcaaattgcc gtttattttt gccaagggaa
     3901 aatgcatcca tctgggtgtg gaggggcgca tcttcgaaga gcgttcgctg acgctcgcgt
     3961 gggtgctgac gtaggccggc ttgggtgacg acctcgcgat tggcgccggc atgcatggcg
     4021 gcgtgcataa cggccgcggg acatactaaa acgttcgttt gcccgttgat atatctgctc
     4081 taaacggaat cctcatgaag aacctctttc tctttgccgg aatgcttttt ttcctgtgtt
     4141 cctgctcttc caccaacctg atgtccctta gtgtgactgt gcctgctcca gtaagcctct
     4201 cgccggctgc gcattcggct gccgtcgtga atagaagcgg cgatgataac agcgcggtcg
     4261 accgtcttca ccggacgctg tcgctcgaga ccaggaatct tcagactgaa ggcgccaggg
     4321 cgagcatgag gggtctggcg gatgagctga taaaagacgg ccgcttcgca aatgtcatac
     4381 gtttggatag tctggatctg cgttcttatg gtgcgggggt atttcccact tatctgtcgt
     4441 gggataccgt ggaaaggata tgcaggcaga atcatgcgga tgtactgttc tcgctggagt
     4501 tgttcgatgc tgcatcgaag gtgaatgtcg gatcggctcc cgcgcttctg gcaaacccgc
     4561 ttggtgctgt tgcggtcctc gatcagccgt tcggcgtaaa cacccgtgtc aagacgggct
     4621 ggaggatata tgatcccgct actcgaaata tactcgatga atatgtgctc tataaggacc
     4681 ttgattttca gggtcgcgtc ggcaatccgg tgcgggcagc caattccatg ttgggccgcc
     4741 aggaggccgt aagacaggca ggtatcgagg caggacagtc gtatgcctta cggatcgtgc
     4801 cttattccat ccgggtgtcg cggttttatt ttgtgcgggg gaacgggaat tttatcatcg
     4861 ccaaacgcat ggcgcagtcg ggggattggg atggcgccgc aaggatatgg cagcaggagt
     4921 ctacgtcttc ttcgcggaag gtcgccggac gggcctgtta taatatggcg atcatcaggg
     4981 agatcaatgg ggatctgaac ggtgctgtga catggtctac caaggcatat gaagtgtata
     5041 atgtccggtt ggccttaagc tatttgagcg ttcttcataa caggcagaat gaggacgctg
     5101 ttttaaaaag tcaaactgag ttgacgggga attagtcgga cgggcagccc gccggcggca
     5161 tgcggctgtg cgacggctat tcggtgagaa gatcatcgtc ctggtcttcg agcagcggga
     5221 catcgatgac gtctttacct gcgatgcctt cgatggagcc gcggacatgg gccgcattga
     5281 gcagcacttt ctccagctgt ttttcccttg ctttccagag ccgttccata gtatctcttt
     5341 ccttttgaat ggattgtttc atcgagagga atccttcccg gatggctttc cactgttcgc
     5401 tgaattcggc gctggtcaga taattataca gcagggtcat cttgtcgcct ttattctcct
     5461 gggatttggt gatattgaag attcgaatta tcccatctct gagcacgtga gctaatgctt
     5521 ttacttcctt aaaggagcat acccaaacac catttatttc gccgaatcgt tccaaatcct
     5581 tcggcatggt ctgcgtaaca atgacggcga ccacggcccc ctggccgcgc atatcagccc
     5641 ttaacttctc cacccactcc tgggagaaag attgagttcg tttactttca tagatgatcc
     5701 tgccgcattc tagtcctgat ttattaaaaa ctgtttgtat acaatctgcc ccgcgcacgc
     5761 ctttaccaac ctccgagacc ttatcgaacg gaaatgcaga ccgtaataat tcttccaaag
     5821 caagctcctg ggattcacct tgtaattgca ttgacccttg ctcagccttg cgccgcatct
     5881 cctccgccaa ttttctttga tcgtcgagtt gtttttccaa ctcttttaac cgcaagcgaa
     5941 attccgtttc ctttgctgca attttttgtt cttccaaatc acgaagttcc ttggatagtt
     6001 tgactctttc ttcttgtagc aaccgctgaa cagctagttc cagttcggcc tccttggttt
     6061 gtaattcctg ctcctttttg aggaattcga attcgcgttg tcgagacact cttagctttt
     6121 cctcggtgtc ctttcgagca tcttccagca agcgaagctt gttttcgtaa tcgctgccaa
     6181 ttgagttgcg caatgacttc tctaacgtgg tttgcaaatt gcttttctct tcctccaatt
     6241 tgcgaacgaa agaagcttcc ttgtcccgaa tgaatttttg catctgctcc cgtaggtcct
     6301 tcttatattc ttctgccacg gcgtcttcca tggggaattg atggccgcag ttcgggcatt
     6361 ttatttctgt tggcatctgt ttttatttct gggtaaagat aagttgattt ttggtggaac
     6421 tcacatatga tgaaattcta gaagtcaaag cttcattgca aagctacgtc gataaagtaa
     6481 ggatggagat tgaaataata gagggcgctc tccagctatt gactaaaata tctaataata
     6541 aaaagataaa cgtctccaca atctttctgc cagaggaaat cctaaggttg aatgaagtca
     6601 aagcgaataa gaagcccggc attgaatgga agaaggcggt ttatgagacc atcagaaaat
     6661 caaatttttt tttaagcagc aaaatgattt acgatcgcat gcttgtctat cacccgtttc
     6721 aacttggtga taagcgatac tcgcaaaaat gtataagctc cgctttagtg aatcttgttg
     6781 cagatggaaa agtggggaaa ttcaaagatg agtctggatt atactttttt ggcgatacgg
     6841 agaggcattt cgaagcaact ggcaagccta atcttcaatt tttcaccaac acataaggtt
     6901 ctctgctcca acgattaatg aatggaccgt tggatgaaat tttaattatt gaatttgaaa
     6961 ttcactttta aatgaatacc tttacacttg agctatggaa tgatgagggt aagaagtgta
     7021 ttttctatac tgtccgttgg caggacgcga gaatgaatga ggcggatagg ttttttgaaa
     7081 aatattatgc ggtagatgaa
//
LOCUS       DASYSV010000135         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2331500, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000135 DASYSV010000000
VERSION     DASYSV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331500"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..2737
                     /locus_tag="VGQ51_04280"
     CDS             35..2737
                     /locus_tag="VGQ51_04280"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9035807.1"
                     /translation="MLPTFQGMTRRKRPNELDRTPSDRLPPLVAVLLFILFSFLTTPS
                     TAQDQKFAQTLVTVTLKNASFPEALSVIESKCPYKFAYDAGILPAQHRVSLTAVQMPL
                     QELLRQVLVGTTLTYQVVGDQIILQSPHAASTVTLSGYVKDTRTGELLIGASIYEPFS
                     GHGILSNNYGFYSLSVPVSDTLNLEISYVGYKTLSRRVNLSGDEMLSFELEHNDDQES
                     IGKLTISKDKREDNVKKNQTALVDLSNDMIVSAPSVSGSGDVISSVEMLPGVQAGIDG
                     SPGYYVRGGNEGQNLILLDDATLYNPSHIFGLVGIFNPPTVKYASLMKGGFPAIYGDH
                     ISSVLNVVMKDGSNQQTGGNVQMGTLSSGATVYGPLEKGKSSYLISGRRSMTDVLLHP
                     LLQNNYFKDYYFYDVNAKLSFQLSPKDRLLVGFYSGRDNNTYSGNSTDSTGIDYSMNF
                     GNTTFMLRWNHQFSGKLFSHTSVEYNRYHQFLSATEDGFFAQLYSGIKDINAKSEIDW
                     YPASGHTITAGVDYLHQALYPASLSGQVAPPDSTLNIVPSGIPPKTNNRVAAYVGDNI
                     VLGKKWQAYVGVRAPYFFKDDATYFSVEPRLSLLYLIDPTTSVKVSFTSMHQYIHLVQ
                     SYNASFPAEIWIGSSNVVQPQTSTEYTAGVYKNFLSNILQTSLEVYYKQMGNQLLFGG
                     VDTPVIDNSVESQLVFGKAWSYGAELFVRKNRGRWTGWLAYTLAYAYQQFDSLNEGQT
                     FPFAYDRRNMVDLSTAYAITPHWKIAANFLAASGRAFSLSPDSSYIINPGPGHSPLFD
                     NPGRGRGQGRGLNRGKTTLDIIANNYRLTPYNRLDLSLHYQRSRTIGRRTLLSEWIFS
                     VYNVYARPNNSFVYRTIDPSTRKVIAKQLPLIPVIPSITYELTF"
     gene            2833..3645
                     /locus_tag="VGQ51_04285"
     CDS             2833..3645
                     /locus_tag="VGQ51_04285"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEV9035808.1"
                     /translation="MKKPSLKKTLLISTLLFAGLFLFFSCSKSTSRTSATGHLEIRLT
                     DGPATYDAVYIDVQQVAVNVSSDSATDSGWHTIPLLRPGIYNLLDFKNGIDTVLASAD
                     LPAGKLSQLRLILGDGNSVVVDGQSYPMKTPSGQQSGVKLKINTTLTGGIVYRLWIDF
                     DAGRSIVATGSGKYILKPVIRTYADAIGGSIKGFVLPAAAKPEVWAVQGTDTLMALPD
                     TATGYYFFAGVNAGAWNLLFHAQDTAYSDTTSAISVSTGVVTDAGTVTLRKK"
     gene            3749..>5397
                     /locus_tag="VGQ51_04290"
     CDS             3749..>5397
                     /locus_tag="VGQ51_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035809.1"
                     /translation="MKQAIRFLSIAISLAPLHGYGQLNPGGIFANFGVDADTRTNWVK
                     YGPVKGAISTDDWFAPSGVGNNVIDTSNAAVYRADLQGGLNVSFNKRMSTLLYSKVGG
                     KLWLDAVYGRDFSAASTLKDSTTFTIASKNGDNPSNWVGGVSSFPNKNDLVDVYAHMR
                     RDGTSVYDSLWFFTAVSTYGTVGSSYFDVELYKNSFTYSSATGKFTTAGPDAGHTQWL
                     FDASGNILQTGDMILAVNISPGLTPVIDLRIWVSSNTLATVTPARFNFTGAFNGASAS
                     PAFGYASIVSKTGSTAWGAGISNFSVTPTQDTTYATPWGTGTTLGASVWNNQYSTEQF
                     NEIGLNLSRVGVDPALYSALSPCQSLFANIFFKSRSSNSFTSNMQDFVTPLTFLQNPV
                     MDYTLKPDTIRCNHPEGTITLTNNSTVGIYTWKMLSGQIVGSNADSSQLNIDKPGTYV
                     VTASPAEGCPPTRVDTVIVPIDTFPPIPSAFSGLVGSQIALYGGNAIASNYPTPFGGS
                     DGLLYAWSGPNGFAATSQNPLTGDTAWGTYNVTVVEKRNGCP"
BASE COUNT         1234 a         1560 c         1331 g         1272 t
ORIGIN      
        1 cgatcaagta aataggccgg gaataataac tttcttgcta ccgacgtttc aaggcatgac
       61 ccgccgcaaa cgccccaatg aattggatcg gactccgtcc gatcgtctgc ccccgctggt
      121 cgcagtactt ctttttattc ttttctcctt tttaaccacg ccctcgaccg cccaggatca
      181 gaaatttgcc caaaccctcg ttaccgtcac cctgaaaaac gcttcctttc ccgaggcgct
      241 ctcggtcatc gagtcgaagt gcccgtataa attcgcttac gacgccggta tcctgcccgc
      301 tcagcaccgc gtcagcctta cggccgtgca gatgccgtta caagagcttt tgcgccaggt
      361 actggttggg acgaccctca cctaccaggt ggtcggcgat cagatcattc tgcaatcccc
      421 ccatgcggct tcgaccgtca ccctcagcgg atatgtaaag gacacccgta ccggcgaatt
      481 gctgataggc gccagcattt atgagccttt ctcggggcac ggtatcctct ccaacaacta
      541 tggtttctac tcgctgtccg tcccggtctc cgatacgctc aatctcgaga tatcttatgt
      601 cggctataag actctgtccc gtagggtaaa cctctccggg gatgaaatgc tttcgttcga
      661 attggaacac aacgacgacc aggagtcgat cggcaaactc accatatcga aagacaaaag
      721 agaagataac gtcaaaaaga accagaccgc gctggtggat ctgtccaatg atatgatcgt
      781 ctcggcgccc tccgtcagcg gcagcgggga tgtgatctcg tcggtcgaaa tgcttcccgg
      841 cgtacaggcg ggcatcgacg ggtcaccggg ttattacgta cggggcggca acgaaggtca
      901 gaacctcatc ctgctggacg atgcgacgct ctacaacccc agtcatatct ttggactggt
      961 gggcatcttc aacccgccca ccgtcaaata tgccagcctg atgaaaggtg gctttcccgc
     1021 tatttacggc gatcacatca gctcggtgct gaacgtggtc atgaaagacg gaagcaatca
     1081 gcaaacgggc gggaacgtcc agatgggcac gctttcttcc ggcgccacgg tttatggacc
     1141 cctggagaag ggtaaatcct cttacctgat ctccggccgc cggagtatga cggatgtcct
     1201 gcttcatccc ctgctgcaga acaactattt caaagactat tatttctacg acgtcaatgc
     1261 caagctcagc ttccagctgt ctccgaagga tcgtcttctc gtcggctttt acagcggccg
     1321 ggacaacaat acctattccg gcaattcgac ggacagcacg ggcatcgatt attccatgaa
     1381 tttcggtaat acgaccttca tgttgcgatg gaaccatcaa ttctcgggga agctcttttc
     1441 acatacgtcg gttgaatata accggtatca tcaattcctg tcggcgactg aagacgggtt
     1501 cttcgcgcag ctgtattccg gcatcaaaga tatcaacgct aagagcgaga tcgattggta
     1561 tccggcctcc ggccacacca ttactgcggg tgtcgattat ttgcaccagg ccctctatcc
     1621 cgcgtcgctg tcaggacagg tcgctccgcc cgattcgacg cttaatatcg tcccgtcggg
     1681 gataccgccg aaaacgaaca accgcgtcgc ggcgtatgtc ggggacaaca tcgtactcgg
     1741 caaaaaatgg caggcatacg tgggcgttag ggcgccttat tttttcaagg acgacgcaac
     1801 ctatttttcg gtggaaccgc gtctgtccct cctttacctg atcgatccta ctaccagcgt
     1861 aaaagtctcc ttcaccagca tgcaccagta catccatttg gtgcagagct ataacgcttc
     1921 cttccccgcc gagatctgga tcggcagcag caatgtcgtg caaccgcaga cctccacgga
     1981 atacaccgcg ggagtataca agaatttttt gtccaatatc ctgcagacca gtctggaagt
     2041 ctattataag caaatgggga accaattgct tttcggaggg gtcgatacgc ctgtcatcga
     2101 taactccgtc gaaagccagc ttgtcttcgg aaaagcgtgg agctatggcg ccgaactttt
     2161 cgtgcggaaa aaccggggca gatggactgg atggctggct tacacgctgg cttatgcgta
     2221 ccagcaattc gactctctca acgaaggaca aacctttcct tttgcctacg accggcggaa
     2281 tatggtggat ctttcgacgg cctatgccat taccccgcat tggaagatcg cggccaattt
     2341 cctggcggcc agcggccggg ctttttcgct gagtcccgat tcgtcctata tcatcaaccc
     2401 gggacccggg catagcccgc tattcgataa ccccggcagg ggccgtggac aggggcgtgg
     2461 tctcaatcgt ggcaagacca ccctcgacat catcgccaac aactatcgcc tgaccccgta
     2521 taatcgtctg gacctcagcc ttcactacca aagatcgcgg actataggcc gaaggaccct
     2581 cttatcggaa tggatattct cggtatacaa tgtctacgcc cggccgaata actccttcgt
     2641 ttacaggaca attgatcctt ccacgcggaa agtgatcgcc aaacagctac ctcttatccc
     2701 tgttattcct agcattacgt atgagctaac gttttaatac aaaaaaacaa aaataatact
     2761 tcccgaccta ggaccacggg gctattatcc tgtcttatat acgtaagcct tcatgataaa
     2821 attctgaatc tcatgaaaaa accgagcctt aagaaaactc tgctaatctc cactctcctg
     2881 tttgcaggtc tgttcctttt cttttcctgc agtaaaagca caagcagaac cagtgctacg
     2941 ggtcatctcg aaatcagatt gacggacgga ccggcaactt acgacgcggt ttatatcgac
     3001 gttcagcagg tagcggtcaa cgtttcctcc gactcggcaa ccgattccgg ctggcatact
     3061 atacccttac ttcgacccgg catatacaac ctgctcgatt tcaaaaatgg gatcgacacg
     3121 gtattggcct ctgccgacct gcccgcgggc aaattgtccc agctgcggct gatcctcggg
     3181 gatggcaatt ctgtggtcgt cgacggccag tcctatccga tgaaaactcc ttccggacaa
     3241 caatccggag tgaagctcaa gatcaatacg acgctcaccg gcggtatcgt ctaccgtctg
     3301 tggatcgatt tcgacgcggg tcgctcgatc gtggcgaccg gctcaggaaa atatattttg
     3361 aaaccggtga tccggaccta tgcagatgcc attggcggca gtatcaaggg ctttgttttg
     3421 cctgcggccg ccaaacccga agtttgggca gtgcagggca cggatacgct gatggcccta
     3481 cccgacacgg caaccggtta ttactttttt gccggcgtaa atgccggcgc ctggaatctc
     3541 ttatttcacg cacaggatac tgcctattcc gacacaacat ccgctatatc agttagtacg
     3601 ggtgttgtga cggacgcagg aacggtgacc ctgcgtaaga aataaggcac ctatgaaacc
     3661 taaggcgatc cgggcatact ttgcccggat ttgtcccgtt tataaataag gcgatctacc
     3721 gtagggtcgt ctcacataat ccgaagctat gaaacaagct atccgattcc tctcgatcgc
     3781 gatcagcctt gcacccctgc atggctatgg ccagctgaac cccggcggca ttttcgcgaa
     3841 ctttggtgtg gacgccgata cgcgtaccaa ttgggtgaaa tatggtccgg tcaaaggcgc
     3901 catatccaca gacgactggt tcgcgccgtc aggcgtcggc aacaacgtca tcgacacgtc
     3961 caacgcggcc gtttatcgcg ccgatctgca gggtggtctc aatgtctcct tcaacaaacg
     4021 aatgtcgacg ctgctgtatt cgaaggtcgg cggcaaactc tggctggacg cggtctatgg
     4081 aagggacttt tcggcggcct ccacgcttaa ggattcgaca acgtttacca ttgcgtcaaa
     4141 aaacggagat aacccatcca actgggtagg cggcgtttcc agcttcccca ataaaaacga
     4201 cctggtagac gtctatgccc atatgcgcag ggacggaaca tcggtgtacg attcgctttg
     4261 gttctttacc gcggtctcca cctatggtac ggttggatcc agctatttcg atgtcgagct
     4321 ctataagaat tcctttacct atagctctgc gaccggtaaa tttacgacag ccgggccgga
     4381 tgcaggacat acccaatggc tcttcgacgc ctccggcaat atcctccaga cgggtgatat
     4441 gatcctggca gtaaatattt cgccgggcct gacccctgtg atagacttgc gcatctgggt
     4501 cagctcgaac acattggcga cggttacgcc tgcccgtttc aattttaccg gcgccttcaa
     4561 tggcgcatcg gcgtctcccg ccttcggata tgcttccatc gtctcgaaaa ccggttccac
     4621 cgcctggggc gcaggtattt ccaacttttc cgttactcct acacaggata ccacctatgc
     4681 caccccctgg gggacgggta caacgctcgg agcctctgtt tggaataatc aatactcgac
     4741 agaacagttc aacgagatcg gtctgaatct gtcccgtgtc ggtgtcgatc ccgcgctgta
     4801 ttccgctttg agcccctgtc agtcgctgtt cgccaatatc ttctttaaat cgaggtcctc
     4861 caattcgttt acttcgaata tgcaggactt tgttacgccg ttgaccttcc tgcagaaccc
     4921 cgtgatggat tatacgctga aaccggatac catccggtgt aaccatcccg aggggacgat
     4981 cactctgacc aataacagta ccgtgggtat ctatacctgg aagatgctga gcggccagat
     5041 cgtggggagc aacgccgaca gctcgcagct caacatcgac aaaccgggta cctatgtcgt
     5101 cacggcatcg cctgccgaag gctgtccgcc gacgcgcgta gatacggtaa tcgttcctat
     5161 cgataccttc cctcctatcc cctcggcctt ctcggggttg gtaggcagcc agatagcgct
     5221 ctatggcggc aatgccattg ccagcaacta tcccacaccg tttggaggtt ccgatgggtt
     5281 attatatgcc tggagcggac cgaacggttt tgcagctacg tcccagaacc cgctcaccgg
     5341 cgatactgcc tggggtacgt ataatgtaac ggtggtggaa aagcggaacg gctgtcc
//
LOCUS       DASYSV010000136         9555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_923087, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000136 DASYSV010000000
VERSION     DASYSV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923087"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1576)
                     /locus_tag="VGQ51_04295"
     CDS             complement(26..1576)
                     /locus_tag="VGQ51_04295"
                     /inference="COORDINATES: protein motif:HMM:NF037325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030247 - polysaccharide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycan-binding surface protein"
                     /protein_id="HEV9035810.1"
                     /translation="MKRLLLFLVSLAALAQFACKKSESGMPTVTHVRLVDPTKADSFF
                     TQAKPGTLIAIQGSGFAGLQAVYFNDTSAYFNPVYATNTNIIITIPGYAQTLATKSNA
                     TNTIKIVTGHGTTTYAFKLVLDPPSISSITLDNTGKLLYINGYNLLGVKKITFPVPNP
                     DTALGYTVDTSGRLVTAVIPPGLASNDSIRVYCTFGVASFPYPPPMTISSVSNENAIL
                     GDTITITGTNFSGISSVTFPGGIVVNNPKVLSISQMTVAVPAGITAPDSLRIAGALGT
                     ATAPQLFDSYITHPSPGYLSTFDVQYTVTNTDNNGFVGWTGGYANAPASAYPNATGGV
                     AFLANGSPIAGNTGGGSQGNPGFIQLYDVPWVANTGDNPSNYSLKFEVYVKGSWKAGA
                     IYVMMGDWYGWKGYLARYAPWTTDPNANFSPGAWVTATIPLSQMVSTTGPGTSVMANG
                     KVASPNNDANEWDYQTFPTGGMPAANFTTYNSTALCFAIVNDQASPAIAANALNIAID
                     NIRIVKGK"
     gene            complement(1617..3338)
                     /locus_tag="VGQ51_04300"
     CDS             complement(1617..3338)
                     /locus_tag="VGQ51_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9035811.1"
                     /translation="MNKIITILSIMLLGAAILPGCKKNFLNRPPLSSPTSGTFYQTDA
                     DILGGTAVQYNASWSGYNGTSLNYIGDVLGGDMVIDNYNGRGAYMNFTVSSADGSGAL
                     QSAYQAFWYVVANANVIAANIKAAGAGASQAARSEGLAECYFMRAAAYYYLTLNWGAV
                     PIIYDNSSQIGDSTIHRNLLSDDWKFMINDLLFAMKNLPATPVQPGRITKWSAEGMLA
                     RAYLVRSGLTGSGGSRNQSDLDSAKLYAGDVCHNSGLTLEPNYYDLFTSGKFSGTTVP
                     SESLFSLMWVPTGQWFVQNHMQANLAYDSKITQTGDGWGGSFGGSASLMAYYMDPANA
                     ADTMRRRATVFMPGDAYSDINKPAGWLVDTSLFNNAKIYVAGQQGNGYGNDRAYVKKY
                     VIGSPSDNGGQGAQQDENLNTYMFRLADVYLIYADAILGNNPSTTDAEALTYFNAVRS
                     RAGATTKTSITYADLLLERKIEFAFEGHVWYDWKAWYYFDPTDALNYFSNQNRGNYNI
                     SYNSGHPFITFFNSNGLPNADGSAGSVHYQITQQTADLPFPEAELLVAPALGQAPVPF
                     DFSKVKY"
     gene            complement(3362..6556)
                     /locus_tag="VGQ51_04305"
     CDS             complement(3362..6556)
                     /locus_tag="VGQ51_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9035812.1"
                     /translation="MKKLRLLANVAIFVPLAALLLFAVPLSAQSPAAHRVTGKVTAQS
                     NGSAVGGATVSVKGTKNAVQADDNGDFSIMAAPGEHLLVSYVGFTTKDIRLGNSTVVN
                     IELSQDISNLSDVVVVGYGKMKKSDLSAAVGTISNSDLEKTINVTLDDAIQGKAANVY
                     VSQTSGAPGAGASVIIRGVSTVTGNYQPLYVIDGVQIRPQLPTGGAYNVTPNLANELQ
                     GLNPDDIENISVLEGPAATSIYGAAGANGVIMITTKQGKAGQTRVNFNELQNLQTRPK
                     DLPVMNLQQYAVYVNKLQGLGLVPTEPSELSDPSLLGPGTDWQHALFQNVWMQKHSLG
                     VSGGNDKTTFYISGDYLNQNGVAIGSGFKRGSVRMNITNQANKWLRLGLNLSGFGTRE
                     TSNIFQGNLINVAIGQNPTIPVKNPDGTFGGPAPAQAVYSNTNPVAVATLNHNYNTSY
                     GGIGGVYIDITPVKGLTWHTEANGNISFSQNFSFNPSYTIGSYVNGTTTGNRGAGNNW
                     WGSLNSRLQYDYTNGKHQASLMVGHEASEYAYQNLFANAQDWSTNSIEELSAASNIIS
                     NPTSGRGDGAQESYIARANYTYDNKYIAQLVFRRDGSSNFGPQNQWGNFPAASVAWKI
                     SDEKFMQTISWLNDLKFRFEYGISGNAGPGGAIYSNLYSTSTVWGTGFAPANFANPAL
                     QWEQDKSANVGLDAHLFNNRVEIIADAYKKTISNLILPASGQEFLGGVLNGGYGGMIT
                     WPIKNYGGMQNEGIGITVNTDNISTHDFQWKTGVNFSIDKNKVTKLYAPIYTQYYSTT
                     NSRQAEFLTEVGQPLSMITGYIADGLFMNYKDISGHAIQTSNGVMTISPTQGSWPGDV
                     KFRDINKDGLVDNNDRTIIGNPWPKFTANFNTNFTFKGFELNLFFTGVYGNKILNLTR
                     YQNEYIPLGVGPYSNHFRSDVNFAVPSSTNIADALTATLTNPGHQIPRPSQADANQNV
                     RLTQWDVEDGSYIKLKNARISYSVPSRILQTTHVLRGVIASFQCQNVFTVTKYKGYDP
                     EVGMYQYGGFNIVGMDEGRYPQTRSYAFSLSLNF"
     gene            6763..>9555
                     /locus_tag="VGQ51_04310"
     CDS             6763..>9555
                     /locus_tag="VGQ51_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9035813.1"
                     /translation="MRLQKTLLFILCCLTINLLSAQPEEYLFSHVDVTYGLSNNHITS
                     IYKDARGFMWFGTVSGLNRYDGYQTRVFKHDPQDPHSIADNYIEQIFEGPGGKMWVES
                     RKGRYNIYDACLDRFEGDFSSYLKKLGLPVYELLTITPSKSGYWFIYRDSGIFHYMPN
                     GKIVAFRPDALSGLTVAAAREDGNGNCWVVHENGLLEEIDGRQNKVIFRLPALEKEFG
                     NVPMTACRLFIDAQNDLWLFSNGVFKGTYYYHPPTHELRHFALDAGERRLNSNIVLTA
                     LQDKKGLIWLATDHGGVDIVDKRNFSVKFVTHVEDDNKSIAGNSITSLYRDNGGTVWL
                     GTYKNGISFYHQDNFQFPQYRHEPNRPGTLPFEDVNSFAEDEAGNIWIGANGGGLIYF
                     DRKKNSFRQWLHDPGNPNSLCSNIIVSLLRDRDGRLWIGTYFGGLDCFDGQHFIHYRH
                     DDNDPYSLADDRVMCLCEDGDGQLWVGTLSAGMDRLDQRRKVFYHYRANLPNSIHNDY
                     VSSIITDKQDNLWIATGWGIDVIEKNTGDIRHYGVDYNQLSSDNVTWLYMDSKQRLWA
                     ATREGLDVLTPDEKMFQAFTTEDGLPDNTIRDIEEDSLHRLWVSTANGLSRIAVVPTG
                     DHGSLRIRCRNFHEQDGLQGREFNERTGLVTKDGSLLFGGPNGFNIFRPEDIAPGRRT
                     PPIVLTGLEVFDKTIKVGDQAGGHVILQKALTETGDITLTHHDNVFSIEFASLGYIPN
                     ATNRYAYELEGFNHNWLITDGKIRKATYTNLDAGDYLFRVKASDEDGEWYDKEATLRI
                     TVLPPWWKTELAYILYFFMLAGSLLFARRMVLRRARVRFALENERRETRRMHEMDMMK
                     IRFFTNMSHELRTPLSLILAPVDKLLNQTPQSEPRRQYEMIRRNARRLLHLVNQLLDF
                     RKMEEGELKLHLREGDV"
BASE COUNT         2154 a         2518 c         2654 g         2229 t
ORIGIN      
        1 tcttaatttt catagttaca atttgttatt tccctttcac gatccggata ttgtcgatag
       61 caatgttcag cgcattcgcc gcaatggccg gcgaagcctg gtcattcaca atggcaaaac
      121 acagtgccgt cgaattatag gtcgtgaagt tggccgccgg cattccgccg gtggggaaag
      181 tctgataatc ccattcattg gcgtcgttgt tgggcgaagc aaccttgcca ttcgccatga
      241 cgctggtacc cgggccggtc gtagacacca tttgactgag cggtatggtc gccgtaaccc
      301 aggctcccgg actgaaattg gcgttgggat cggtagtcca gggcgcatat cttgcgaggt
      361 aacctttcca tccgtaccaa tcacccatca taacgtagat cgcgccggcc ttccagctcc
      421 ctttgacata gacctcgaac ttcagcgagt agttgctcgg gttatcgcct gtattggcga
      481 cccagggcac atcgtacaat tggataaatc cggggttacc ctgcgatccg cctccggtat
      541 tacccgcgat cggacttcca ttcgcaagga aagcgacacc gccggtggca ttgggatagg
      601 cggatgccgg ggcgttcgca taacctcccg tccatcctac gaagccatta ttgtcggtat
      661 tcgtgaccgt atactgcacg tcgaaggtgg agagatagcc gggtgaagga tgtgtgatat
      721 aggaatcgaa cagctgaggg gcggtagccg ttcccagcgc gcccgcaatt cgtaacgaat
      781 ccggagccgt aatacccgcc ggcaccgcaa ctgtcatctg gctgatgctg agtaccttcg
      841 gattgttgac cacgataccg ccggggaagg tgacgctcga gataccgctg aaattggttc
      901 ctgtgatggt gatggtatca cccaaaatgg cattctcatt cgagacggaa ctgatcgtca
      961 ttggcggcgg ataggggaag gaggcgaccc caaacgtgca atacaccctg atggaatcgt
     1021 tgctggcgag accgggtggg atcaccgcgg tgaccagtct gccgctggtg tctaccgtat
     1081 agccaagcgc agtgtccggg ttgggcacgg ggaaggtgat ctttttcaca ccgaggagat
     1141 tgtaaccgtt gatgtacaac agctttcccg tattgtccag cgtgatcgaa ctgatcgacg
     1201 gaggatccag caccagtttg aacgcatagg tcgtcgttcc gtgaccggtg acgatcttga
     1261 tggtattcgt tgcattcgat ttcgtagcca aggtctgggc atagcccgga atggtgatga
     1321 tgatattcgt atttgtcgcg tatacaggat tgaaataggc ggacgtgtca ttgaaataca
     1381 cggcctgcag cccggcaaaa ccactcccct gtatcgcgat gagggtcccc ggtttggcct
     1441 gcgtaaagaa actgtccgct ttcgtagggt ctacgagacg tacgtgggta acggtgggca
     1501 tcccgctttc gcttttttta caggcgaatt gcgcaagggc cgccagggaa acaagaaata
     1561 gcaacaatcg tttcataatt tctattttta attagggcgg ggggccgcct tggcaatcaa
     1621 tatttcactt tactaaaatc gaaaggcacc ggagcctgtc ccagtgcagg cgctaccagc
     1681 aattccgctt cggggaacgg caggtccgca gtttgctgcg tgatctggta atgcaccgat
     1741 ccggccgaac cgtccgcatt gggcaatccg ttcgaattga aaaaagtgat gaacggatgt
     1801 ccgctattgt agctgatatt ataattgccc ctgttctggt tgctgaaata gttcagtgca
     1861 tcggtcggat cgaaataata ccaggctttc cagtcgtacc atacatgtcc ttcgaaagcg
     1921 aactcgatct ttctttcgag aaggaggtcc gcataggtga tggacgtctt tgtcgtcgcc
     1981 cctgcccgcg agcggacggc attgaaataa gtcaacgcct cggcgtcggt ggtcgaagga
     2041 ttgttgccca ggatagcgtc tgcatagatg agatatacat ccgccagccg gaacatatag
     2101 gtattgaggt tctcgtcctg ctgcgcgccc tgtccgccat tgtcggatgg cgatccgatg
     2161 acatatttct tgacatacgc gcggtcgttg ccgtagccat tgccttgctg gccggcgaca
     2221 tagatcttcg cgttgttgaa cagggaagta tcgaccagcc agcccgctgg cttgttgata
     2281 tccgaataag catcgcctgg cataaagacc gtagcccttc tgcgcatcgt gtccgccgca
     2341 ttggccgggt ccatatagta cgccatgaga ctggccgaac cgccgaacga tccgccccat
     2401 ccgtcaccgg tctgggtgat cttcgagtca tacgcgagat tcgcctgcat atgattctgg
     2461 acgaaccact gaccggtagg cacccacatc agcgagaaaa gactttcgga aggcaccgtc
     2521 gtcccgctga attttccgga ggtgaaaaga tcgtaatagt tgggttcgag cgtgagaccg
     2581 ctgttatgac agacatcgcc cgcataaagt ttggcgctgt ccagatcgct ttgattgcgc
     2641 gaaccgcccg acccggtcaa tcccgaacgg acgagatagg cgcgcgccag catgccttcc
     2701 gcactccatt tggtaatgcg tccgggctgg acaggcgtgg caggcagatt cttcattgca
     2761 aacagcaggt cattgatcat gaacttccaa tcatccgaca ggagattacg gtggatagtg
     2821 gaatcgccga tctgcgagct gttgtcatag atgatcggca ctgcgcccca gttcagcgtg
     2881 agatagtaat aggcggcggc gcgcatgaaa tagcattccg ccaacccttc gctccttgct
     2941 gcctgcgatg cgccggcgcc tgcggccttg atattggccg cgatgacatt ggcgttggct
     3001 acgacatacc agaaggcctg atacgcgctc tgcagcgcgc cgctaccgtc ggcggagcta
     3061 acggtgaagt tcatgtacgc gccccttccg ttgtagttgt cgatgaccat atcccctcct
     3121 aacacatcgc cgatataatt cagcgaagtt ccgttgtaac cggaccagga tgcattatac
     3181 tgtacggcag tgcctcccag gatatcggca tcggtctggt agaaggtacc ggaggtcggc
     3241 gacgaaagcg gcggccggtt caaaaaattc tttttacaac ccgggaggat ggccgccccg
     3301 agaagcatta tagaaaggat ggttattatc ttgttcatac cttttgaatt aaatgttgtg
     3361 atcaaaagtt aagtgaaaga ctgaatgcat aagaccttgt ctgaggataa cggccttcgt
     3421 ccatgcctac gatattgaat ccaccgtact gatacatgcc gacttccggg tcataacctt
     3481 tgtatttcgt gacggtgaat acgttctgac actggaacga cgctatcacg ccgcgcagca
     3541 catgcgtggt ctgcaatatc ctggatggta cgctatagct gatcctggcg ttcttgagct
     3601 tgatatagct gccgtcttcc acatcccatt gcgtgagccg cacattctgg ttggcatcgg
     3661 cttgcgatgg cctggggatc tggtggccgg gattggtcag cgtggcggtc agcgcgtccg
     3721 ctatattcgt gctgctgggc acggcgaaat tgacatccga ccggaagtgg ttggagtaag
     3781 gacctacgcc caacggaata tattcgttct ggtatctcgt caggttgagg atcttgttgc
     3841 cgtacacacc ggtaaagaac aggttcagct cgaaaccctt gaatgtgaaa ttcgtattga
     3901 aatttgccgt gaatttcggc cagggattgc cgataatggt gcggtcgtta ttatcgacca
     3961 ggccatcctt gttgatgtcc ctgaatttga cgtctcccgg ccaggaaccc tgggtcgggc
     4021 tgatggtcat cacgccgttg gaggtctgta tcgcatggcc gctgatatcc ttataattca
     4081 tgaacagacc atccgcgata taacccgtga tcatgctcag cggctggcca acctcggtga
     4141 ggaattcggc ctggcggctg ttggtagtgg agtaatattg ggtatagatc ggggcgtaca
     4201 gcttggtcac tttgttcttg tcgatcgaga aattgacacc cgttttccat tggaaatcgt
     4261 gggtggagat gttgtccgta ttgaccgtga tcccgatacc ttcgttctgc atgccaccgt
     4321 agttcttgat gggccaggtg atcatgccac cataaccgcc attgagcaca cctccgagga
     4381 actcctgtcc ggaagccggc aggataaggt tggagatcgt ctttttgtag gcatcggcga
     4441 tgatctcgac cctgttgttg aacaggtgcg cgtccagacc gacattggcg gatttgtcct
     4501 gctcccactg caacgcgggg ttggcgaagt tggcaggggc gaaacccgtt ccccagaccg
     4561 tcgatgtaga gtaaaggttt gaatagatgg cgccgccagg acctgcgtta cccgagatgc
     4621 cgtattcgaa acggaacttc aggtcattca gccagctgat ggtctgcatg aatttctcat
     4681 cggagatctt ccaggcgacc gatgcggcgg ggaaattacc ccactggttc tgaggaccga
     4741 agttcgagct gccatccctg cggaagacca gctgtgcgat atacttgtta tcgtaggtgt
     4801 aattggcacg cgcgatatac gattcctgcg cgccgtcgcc gcgtccgctg gtaggattcg
     4861 aaatgatgtt gctggcggcg cttagctcct cgatactgtt ggtcgaccag tcctgcgcat
     4921 tggcgaacag gttctgatag gcatattccg aagcctcatg accgaccatc agcgaagcct
     4981 gatgtttgcc gttggtatag tcgtactgca aacggctatt caacgatccc caccagttgt
     5041 tccccgcgcc cctgttgccc gtcgtagtcc cgtttacata cgagccgatg gtataggagg
     5101 ggttgaaaga aaagttctgg ctgaaggaga tattgccatt ggcctcggtg tgccaggtga
     5161 gacccttgac cggcgtgatg tcgatgtaga ccccaccgat gccgccatag gacgtgttat
     5221 aattatgatt gagcgtggct acggctaccg ggttggtatt ggagtatacc gcctgcgccg
     5281 gagccggacc accgaacgta ccgtcggggt tcttaacggg gatagtgggg ttctgaccta
     5341 tcgcaacatt gatcagattg ccctggaaaa tgttggaggt ttccctggtg ccgaaaccgg
     5401 aaaggttgag accgagcctg agccatttat tagcctggtt ggtgatattc atccggaccg
     5461 atccgcgttt gaaaccggat ccgatcgcga cgccgttctg gttcagatag tcaccggaga
     5521 tataaaaggt ggttttgtcg ttacctccgc tcacacccag cgaatgtttt tgcatccaga
     5581 cgttctggaa aagcgcgtgc tgccagtccg tcccggggcc caggagcgag gggtcgctca
     5641 attcactggg ttcggtcggc accaggccga ggccttgcag cttattgaca tagaccgcat
     5701 attgctgaag attcatcacc ggcaggtcct tgggtctcgt ttgcaggttc tgcagctcgt
     5761 taaaattcac ccgcgtctgt cctgccttgc cctgtttcgt ggtgatcatg atcaccccgt
     5821 tggcgcccgc agcaccatag atagaagtgg cggcaggccc ttcgaggacg ctgatattct
     5881 cgatatcatc ggggttcaga ccctgcaatt cattggccag attgggggtg acattatagg
     5941 cgccgccggt cggcagctgg ggccggatct ggaccccgtc tatgacgtac aacggctggt
     6001 agttacccgt taccgtgctg actccacgta tgatgacgga ggcgccggcg cccggggcac
     6061 cgctggtctg tgatacatat acgttggcag ccttgccctg aatggcgtca tccagggtca
     6121 cgttgatggt cttttccaga tcgctgttcg atatggttcc gaccgcggcg ctcagatcgg
     6181 acttcttcat ctttccgtag ccgacgacca cgacgtcact caggttgctg atatcctggg
     6241 aaagctcgat attcacaacc gtcgaattgc ccagccggat atctttcgtg gtgaagccga
     6301 cataagacac gaggagatgc tcgcccgggg cggccatgat cgaaaagtca ccgttatcat
     6361 ccgcctgaac ggcattcttg gtgcccttga ccgaaacagt cgctcccccg actgccgacc
     6421 cgttggactg ggcggtcacc ttaccggtaa cacgatgggc ggcgggggac tgggcggaga
     6481 gcgggacggc gaaaagcagg agtgcggcga gcggcacaaa gatggccacg ttcgccagta
     6541 atctcaattt tttcatactt aagcaattag tgcaatcggt ttatttttct acataaatgt
     6601 atatagaatg gggttccgca aggggtatta ttttgatcaa aaaggggggc aaaatgtttt
     6661 ttttcctgtc caaaaagata aaatagcagc aaaaaaccgc aacgcattgt ctatatttga
     6721 tataggacga aagcccgagt cccaactgcc atgtgaatct ttatgagact gcaaaagacc
     6781 ctgcttttca ttctttgctg cctgactatc aatctcctgt ccgctcagcc ggaagaatac
     6841 cttttttccc atgtcgatgt cacctacggc ctgtccaata accatatcac cagtatttat
     6901 aaggatgcga gaggatttat gtggttcggc accgtatccg ggctaaatcg ttatgacgga
     6961 taccagaccc gggtattcaa acacgacccc caggatcccc actccatcgc cgacaattat
     7021 atcgaacaga tattcgaagg ccccggcgga aagatgtggg tggagtcgag aaaaggaagg
     7081 tataatatat atgatgcctg tctggaccgg tttgaaggcg atttctcctc ctatctgaaa
     7141 aagctcgggc tgcccgttta cgaattactg actattaccc cctccaaatc aggttattgg
     7201 ttcatctatc gcgatagtgg gatctttcat tatatgccca acgggaagat cgtggcattc
     7261 aggcccgacg ccctgtcggg tctcaccgtg gccgcggcac gcgaggacgg caacgggaac
     7321 tgctgggtgg tccatgagaa tggcctcctc gaagagatcg acggccgtca gaacaaggtg
     7381 atcttcagac tgcccgcgct ggaaaaagag ttcggcaacg taccgatgac ggcttgccgt
     7441 ctattcatag atgcccaaaa tgacctgtgg cttttctcca atggcgtctt caaaggcacc
     7501 tattattatc atccccctac gcatgagctt agacatttcg ctctcgatgc gggcgaacgg
     7561 cggctgaaca gcaatatcgt tctgacagca ctgcaggaca aaaagggact catctggctg
     7621 gccaccgacc acggcggggt cgacatcgtc gacaaacgga acttctcggt aaaattcgtg
     7681 acgcatgtcg aggacgataa caaaagcatt gccgggaaca gcatcacttc gctgtaccgt
     7741 gacaacgggg gcacggtctg gctcggcacc tacaaaaacg gcataagctt ttatcaccag
     7801 gacaatttcc agttcccgca atatcgtcat gaacccaacc gtcccggtac gctacccttc
     7861 gaggacgtca acagttttgc cgaagacgaa gccggcaata tctggatcgg ggccaacggc
     7921 ggcggactca tctatttcga tcgtaaaaag aatagcttcc gccagtggct gcacgacccg
     7981 ggtaacccga acagcctttg cagcaacatc atcgtcagcc tgctgcggga cagggatggg
     8041 cggctgtgga taggcaccta tttcggagga ttggactgtt tcgacggaca acatttcata
     8101 cactaccggc acgatgacaa cgacccgtac agcctcgccg atgacagggt catgtgtctt
     8161 tgcgaggacg gtgacggaca gctgtgggta gggaccctgt cggcgggtat ggacaggctc
     8221 gaccagcgaa gaaaggtatt ttatcattac cgcgcgaacc tgcccaattc cattcacaat
     8281 gattacgtct cctccatcat caccgacaaa caggacaatc tctggatcgc aacgggttgg
     8341 ggcatcgacg tcatcgaaaa gaacaccggc gatatccggc attatggcgt ggattacaat
     8401 cagctgagct cggacaacgt cacgtggttg tacatggaca gcaaacaacg tctctgggcg
     8461 gctacacgcg aaggactgga cgtactcacg cccgacgaaa agatgttcca ggccttcact
     8521 accgaagatg gtctccccga taacactatc cgcgatatcg aggaagacag cctgcatcgt
     8581 ctctgggtca gcacggccaa cggcctttcc aggatagcgg tggtcccgac cggcgaccat
     8641 ggaagtctgc gcatccgctg caggaatttc cacgaacagg atgggcttca gggcagggag
     8701 ttcaatgaaa gaacggggct cgtcacaaag gatggatcgc ttcttttcgg cggacccaac
     8761 gggttcaata ttttccggcc ggaggatatc gcccctggtc gaaggacacc acccatcgta
     8821 ctcacgggtc tggaagtttt cgacaagacg ataaaggtgg gcgaccaggc ggggggccac
     8881 gtcatcctgc agaaggcgct taccgagacc ggcgacatca cgctcacgca ccacgacaat
     8941 gtattctcga tcgaatttgc gtcgctgggt tatatcccca atgcgactaa caggtacgcc
     9001 tatgagctgg aaggctttaa tcataactgg ctgatcacgg acggaaagat ccgaaaggcc
     9061 acttatacca acttagatgc cggggactac ctgtttcggg taaaagcctc cgacgaagat
     9121 ggggaatggt atgacaagga agccacattg cggatcaccg ttctccctcc gtggtggaag
     9181 acggagttgg cctatatcct gtattttttt atgttggctg gaagcttgtt gttcgcgcgc
     9241 aggatggtgc tccggagagc cagggtccgt ttcgccctcg agaacgagcg gcgtgagaca
     9301 cgtcgtatgc acgagatgga tatgatgaag atccgcttct tcaccaatat gagccatgag
     9361 cttcggacac ccttgtcgct catcctcgct cccgttgaca agctgctgaa ccagacgccg
     9421 caatcagaac cacggcgcca gtatgagatg atccgccgga atgcgcgccg cctcctgcac
     9481 ctcgtgaacc agttgctgga tttccgcaaa atggaagaag gcgagctcaa attgcatctc
     9541 cgcgaaggag acgtg
//
LOCUS       DASYSV010000137         6283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1179603, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000137 DASYSV010000000
VERSION     DASYSV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179603"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VGQ51_04315"
     CDS             <1..1083
                     /locus_tag="VGQ51_04315"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEV9035814.1"
                     /translation="EFWHMSKITFNNSNHAFYTSLKTSVNVYFERYEKRRTGNIRLYA
                     KAVILILTAIALYSLLLFMAMPGLVMVICSLFLGLVLACIGFNVMHDANHGSYSSRKW
                     VNDTLGLTLNALGGNSFIWKQKHNIVHHTYTNIDGIDDDIAKSPFIRMCSSQPWVPAH
                     RLQHFYTPFLYAFSSMIWILFQDFEKYFRRKIAGTPLSRMHRADHIIFWASKTGYLLF
                     YVLIPMLLMGWQSWLLFFLSLHIALGLTLSIVFQLAHVVEDTEFESATGDEKQIENEW
                     AIHQVKTTSNFSPGHPIISWLVGGLNYQIEHHLFPRVSHIHYAALSKLVQRECAVFGL
                     PYNSMPTFKAAVASHFRFIRALGRKP"
     gene            1115..1699
                     /locus_tag="VGQ51_04320"
     CDS             1115..1699
                     /locus_tag="VGQ51_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV9035815.1"
                     /translation="MNPCLNFRNVQLRLVVNAGSVLEDDDQQGLVHFMEHMNFNGLRH
                     FPKNELVSYLQSLGLQIGADLNANTGYDATNYILRMPTGDDKRIEKGFTILYYDTWYR
                     PDLEAVVVVGDIDVGDAEKQIIRHFRNHHEGQMKTNEYWLASLSHSWIDQEDPSWMND
                     FYKTVEGLSARDLRETARKYLNTSNYIQVQLLPE"
     gene            complement(1681..2487)
                     /locus_tag="VGQ51_04325"
     CDS             complement(1681..2487)
                     /locus_tag="VGQ51_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035816.1"
                     /translation="MDPAIARIGRIFYGIAIAAFGLLTIYYRDFPYFLIPPKHGWISD
                     HVIVVYLSGALLLLAGVCIVSGKKVLQVSLLLGTVLLSIFCFYFIPYELLVSTNYMHF
                     GAWENAAKTLALAGGAFVIAARYPGAPAGFWKTLIPLGAICFPLTIISFSIDHFLYAE
                     QAAGYVPSWIPNPVFWLYLTGSALLASGVAIVLRIKPRLSAGLLGSMIFIWVLILHIP
                     KAVAESLAGGGGEISSAFLALAYCGIAFVIAGAAQGTEPLPASLTYSGNS"
     gene            complement(2505..3749)
                     /locus_tag="VGQ51_04330"
     CDS             complement(2505..3749)
                     /locus_tag="VGQ51_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9035817.1"
                     /translation="MAAPRKIGELNVFRAFRSKNYTLYFTGRAVSQFGTWMQRTAVLW
                     FVYSMTNSAFLSGVTIFAEQFPSFLFSMFGGIAADRYDRFKIIKITQIASMIQSGLLA
                     VLMMTRHAAIWQVLALSVLLGIINAFDVPARQAMLHDIVAEQSDLSNAISLTTATACL
                     AQLLGPPAAGFIYKSFGPAVCFWINAASFGGVLVSILLMNLPPYRPSATRKKVIKELA
                     EGFSYVRRTPAIGLTILLAAIASLVVLPYNTVLPVFAKVVFHGDSSTFGYITGFVGVG
                     AVIGTIYLASRKPEAHLRRILFFTSVILCVGLILFAMTKNFSLAMFFAVLTGFGGITE
                     FAVCNILVQSESAPDMRGRAIGILLMAFFGMAPLGSLLVGAISQHIGAPYTVLGEGII
                     GVAVILAFAKYLLRPQNRRILQ"
     gene            3797..4735
                     /locus_tag="VGQ51_04335"
     CDS             3797..4735
                     /locus_tag="VGQ51_04335"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEV9035818.1"
                     /translation="MKKYTIAILGVGGVGGYIGGKLAAAQLSSIRVVFIARGQTLDVV
                     RRDGLKLITDEATFIVHPDIISDDPELIGPIDLLVCSVKGYDLRDSLLRYRSCITSAT
                     NLLPLLNGIGIADKVRAILPGVSSWEGLIYIVARQVEKGIVKQTGALREIIFGSQAGT
                     SPGMQRVAGIFGEVGLKATVSTNIELELWRKFLFISVMATLTSFYHSPIGDIRLDAVK
                     AANIPLLVHELQSVAVANGIPVNDQLVAAVLERINGLAAGTTTSMYADYQRGGNTEVE
                     ELTGDVIRLGGRLGVPTPVYTEMYKSLTAKREPIGE"
     gene            complement(4739..>6283)
                     /locus_tag="VGQ51_04340"
     CDS             complement(4739..>6283)
                     /locus_tag="VGQ51_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9035819.1"
                     /translation="KQTYWALAGIALFIIITACINFINLATGQSATRAREVGVRKVLG
                     ARRPQLIGQFIGETALQVLVAVIIGAILAAVLVPRLAVWLDIGIHTSELTAPAVITLL
                     AALVVCIIPLAGLYPAFVQSAYRPAISLKGTPAVSTGGLTLRRGLVIVQFGISQLMII
                     GTIIVARQMDFFQNRDLGFNKDFVVTFPIADSAHRQVLWQQLSAMPAISAISFSSGAP
                     GYMGGAGSYDAPQFGVNGENITEIKFVDEHFMRMFGITLLAGDSIVRSDIKRGDTSAN
                     VVVNETMTQSLHFQDPHKAIGQSIFFGKFRCIITGVVRDFQDESKHKKIRPCVMLYAE
                     NNFGSASVRINPHNMDATIAHIGKLWSALYPDRLFEYEGLDDHIAAFYRQEQKVYTAF
                     RIFSCIAIFIGCIGLYGLIAFATLQRTREVGIRKVLGASVPNILYLFGREFIWLIVLA
                     FVVAAPLAWLAMHSWLTNFAYRIDIGWGTFVIAASVSFLIAALTISYKTIAAAFANPV
                     QSLRTE"
BASE COUNT         1615 a         1565 c         1673 g         1430 t
ORIGIN      
        1 gaattttggc atatgtccaa aataaccttc aacaatagca atcatgcctt ttatacttca
       61 ttaaagacat ctgtaaacgt gtattttgaa cgttatgaaa agagaagaac tggtaatatc
      121 cgtttatatg ccaaggcagt cattcttatc ttaacggcga ttgccttata ttccttattg
      181 ctatttatgg cgatgcccgg gcttgtcatg gtcatctgca gtttgttttt gggtttagta
      241 cttgcctgta tcgggtttaa cgtcatgcat gatgctaacc acggcagcta ttcttcccgt
      301 aaatgggtga acgatacgct tgggctcacg ctgaatgccc tgggcggcaa tagttttatt
      361 tggaaacaaa aacacaacat cgttcatcat acttacacaa acatcgacgg catcgacgat
      421 gatatcgcta aaagcccttt tatccgcatg tgcagttccc agccctgggt accggcgcac
      481 cggctgcaac atttttatac tccattcctg tatgctttca gttccatgat ctggatcctg
      541 ttccaggatt ttgaaaagta tttcagaagg aagattgccg gtacgcctct ttcaagaatg
      601 cacagggcgg atcatatcat cttctgggcg agcaagaccg gttatctgct tttttatgtc
      661 ctgatcccga tgttgctaat gggctggcaa tcctggttgt tatttttttt atcgctgcac
      721 attgctttgg ggcttacatt atccatagtt tttcaattgg cgcatgttgt ggaggatacg
      781 gaattcgagt cggcaaccgg cgatgaaaaa caaattgaaa atgaatgggc catccatcag
      841 gtgaaaacga cttcgaactt ttcacccggc cacccaataa tttcctggtt ggtcggagga
      901 ctaaattatc agatcgaaca tcatttattt ccaagggtaa gtcatatcca ctatgcggca
      961 ttgagcaaac tggtacaaag ggaatgtgcg gtcttcggct taccttataa tagtatgcct
     1021 actttcaaag ccgccgttgc ctctcatttc cggtttatca gggcgctggg aaggaaaccg
     1081 taaaaataac tatctttccc tctcaataac taccatgaac ccctgtctga attttcgcaa
     1141 cgtccagctg cggcttgtgg tcaatgccgg atcggtcctg gaggatgacg accagcaggg
     1201 actcgtgcat ttcatggagc acatgaattt caatggtctc aggcattttc cgaaaaacga
     1261 gctggtcagc tatctgcaat cgctgggtct gcagatcggt gccgatctca atgcgaacac
     1321 cgggtatgat gccaccaact atatcctccg gatgcccacc ggcgacgaca aacgaatcga
     1381 aaagggtttt accatcctct actatgatac ctggtatcgc cccgatctgg aggctgtcgt
     1441 ggtcgtagga gatatcgatg tgggggatgc ggaaaaacag atcatccggc atttcaggaa
     1501 ccatcacgag gggcaaatga agacgaacga atactggctg gccagtttga gccatagctg
     1561 gatcgatcag gaagaccctt catggatgaa tgatttttat aagaccgtcg agggtttatc
     1621 cgcgagggat ctgagagaga cggcgagaaa gtatttgaat acctcgaact atatacaggt
     1681 tcagctgttg ccggaataag tgagagaggc cggtaagggc tcggtcccct gagcagcacc
     1741 ggcgataacg aaagcgatcc cgcaatatgc cagcgctaag aatgcgctgg atatctctcc
     1801 cccaccgcct gccaacgatt cggccacggc ttttgggata tgaagaataa gcacccaaat
     1861 aaagatcatt gatcctagaa gaccggcgct aagcctcggt ttgatcctca aaacgatcgc
     1921 aaccccggag gcaagcaagg ccgaaccggt gagatagagc caaaagacag ggttgggtat
     1981 ccaggaaggg acatagccgg cggcttgttc agcatacagg aaatgatcga tggaaaagct
     2041 gatgatggtt agtgggaagc aaatagctcc caggggtatc agcgttttcc aaaaacctgc
     2101 aggtgcgccc ggatagcgcg ccgcgatgac aaatgctccg ccggccaatg ccaatgtctt
     2161 cgccgcattc tcccatgcgc cgaaatgcat atagttcgtc gataccaata attcatacgg
     2221 gataaaataa aaacaaaaga tcgaaaggag aaccgttccc agcagcagag aaacctgcag
     2281 gacctttttc ccggacacga tgcatacgcc ggccagtaat agtaaagctc cggatagata
     2341 aaccacgatg acatgatcgc taatccagcc atgtttcggc ggtatcagaa aataagggaa
     2401 gtcccggtag tagatcgtca gcaagccaaa tgctgctata gcgatgccat agaaaatgcg
     2461 accgattctc gcaattgccg gatccatggt cctgtcggtt ttggttattg caatatacgg
     2521 cgattttgcg gtcttaggag atatttggcg aaagccagga tgaccgctac cccgatgatt
     2581 ccttcgccga gtaccgtata gggtgctccg atgtgttgcg agatggcgcc tacaagcagg
     2641 ctccccagtg gcgccatccc gaaaaaggcc atcagcagga taccgatggc gcgaccgcgc
     2701 atatccgggg cggattcgga ctggacgagg atattgcaaa cggcaaattc ggtaatccct
     2761 ccgaaacctg tcaggacagc gaagaacatg gccagggaga agtttttggt catagcaaaa
     2821 aggatgagtc ccacgcaaag gatgaccgag gtgaaaaaca agatccgccg cagatgcgct
     2881 tctggtttac gcgaagccag atagatcgta ccgatgacgg cgcctacacc tacgaaaccc
     2941 gtgatatagc caaaggtcga cgagtcgcca tgaaagacca cttttgcaaa aacgggaagt
     3001 accgtattat aaggcaacac taccaggctg gcgatagcag cgagtaaaat cgtcagaccg
     3061 atcgcaggcg ttctccggac atagctaaag ccttccgcaa gctccttaat gacctttttc
     3121 cgggtggccg atgggcgata cggcggcaaa ttcatcaata gtattgaaac aagcactccg
     3181 ccgaaactcg cggcattgat ccaaaaacag acggcgggtc cgaaggattt gtagataaaa
     3241 ccggctgcag gcggacccag cagctgggcg agacaggccg tcgccgtggt cagcgaaatg
     3301 gcgttggaaa gatcggactg ctcggccacg atatcgtgga gcatagcctg tcgggcgggg
     3361 acatcgaagg cattgatgat ccccagcagc acgctcagcg cgagtacctg ccagatggcc
     3421 gcatgacgcg tcatcatcag cactgccaat agacctgatt ggatcatgga ggcgatctgc
     3481 gtgatcttga tgatcttaaa acgatcgtat ctgtctgccg cgatgccgcc gaacatcgag
     3541 aaaagaaaag aaggaaattg ttccgcaaag atcgtgacgc cgctaaggaa ggctgaattc
     3601 gtcatcgaat aaacgaacca caggaccgct gtacgttgca tccaagtgcc gaattgagac
     3661 actgccctgc cggtaaaata tagggtataa ttcttactgc ggaatgctct gaatacattt
     3721 agttcgccga tctttcgagg tgctgccatg gactggcaaa aataccgctc aaaagctgta
     3781 atttgttcca ccaactatga aaaagtatac cattgccatc ctgggtgtcg gcggtgtggg
     3841 cggttatatc ggcggcaaac tggcggcagc gcagctctct tcgataagag tcgtctttat
     3901 cgcacgtggt cagacgctgg acgttgtgcg gcgggatggt ttgaaattga tcaccgacga
     3961 ggcaacattc atcgttcatc ccgacataat aagcgacgat ccggaattga tcggacctat
     4021 cgatctgctg gtttgctccg tcaaaggtta cgatctcagg gacagtcttc tccgatatcg
     4081 atcctgcatc acttctgcca cgaacctatt gccgctgctg aatggtatcg gaattgccga
     4141 taaggtccgg gcgatccttc ccggagtatc ttcctgggaa ggcctcatct atatcgttgc
     4201 acgccaggtc gaaaaaggga tcgtcaaaca aaccggagcc ctgagagaga tcatcttcgg
     4261 atcgcaggca ggaacatcac cgggtatgca gcgggtagcc gggatcttcg gggaagtcgg
     4321 tcttaaggcg accgtcagca cgaacatcga gctggagctg tggcgaaaat tcctttttat
     4381 ctccgtgatg gcgacgctga cttcctttta tcatagtcct ataggtgata tccggctgga
     4441 cgccgtaaag gccgccaata ttccgctatt ggtccatgag ctgcagtcgg tagccgttgc
     4501 caacggcatt ccggtcaacg atcagcttgt cgcggcggtg ctggaaagaa taaacggtct
     4561 ggcggcgggt actaccactt cgatgtatgc cgattatcaa agaggcggca acacagaagt
     4621 cgaagaattg accggagatg ttattcggtt gggcggaagg ctgggtgttc cgacgcccgt
     4681 ctatacggag atgtataaga gcctgacggc taagcgcgag cccatcggtg aatgaccgct
     4741 attccgtcct caggctttgc acggggttgg cgaatgcggc ggctatcgtt ttgtacgaga
     4801 tcgtcagggc cgctatcagg aaggatacac tggccgcgat cacgaaggtc ccccacccta
     4861 tgtcgatgcg gtaggcaaaa ttggtgagcc aggagtgcat agccagccat gccaggggcg
     4921 cggccacgac gaaagccagc acgatcagcc agatgaattc ccgtccgaaa aggtaaagta
     4981 tattcgggac cgaggcgcca agcaccttgc ggatccccac ctcgcgggtt cgttgcaggg
     5041 tcgcgaaggc gatgagcccg tagagtccga tgcaaccgat aaatatggcg atgcaggaga
     5101 atatcctgaa cgcggtgtag accttttgtt cctgccggta aaaagccgca atatggtcat
     5161 cgaggccttc atattcgaac agccggtcgg gatacaacgc cgaccatagc tttccgatat
     5221 gtgcgatcgt ggcatccata ttgtgcggat tgatccgcac gcttgcagac ccgaaattgt
     5281 tttcggcgta gagcatgaca cagggtctta ttttcttgtg tttgctctca tcctggaaat
     5341 cccggaccac acctgttatg atgcaacgga atttgccaaa gaaaatactc tgaccgatgg
     5401 ccttatgagg atcctggaaa tgcagacttt gggtcatcgt ttcattgacg actacattcg
     5461 cagaggtatc gccccgcttt atatcggacc tgacgatgga atcgccggca agcaaggtta
     5521 tcccgaacat ccgcataaaa tgttcgtcga cgaattttat ttccgtgata ttctcgccgt
     5581 taacgccgaa ctgcggcgcg tcatagctgc ccgcgccacc catatacccg ggcgctcccg
     5641 aagaaaagct gatcgcggat attgccggca tagcggaaag ctgttgccac aatacctgtc
     5701 ggtgtgccga gtcggctata gggaacgtga cgacgaaatc cttgttgaaa cccagatcgc
     5761 ggttctgaaa aaaatccatc tggcgggcca cgatgatcgt gccgatgatc atcagctgag
     5821 aaattccgaa ctgtacgata acaaggcccc tccgtagggt cagaccacct gtggatacgg
     5881 cgggtgtccc cttcagcgat atggcgggac gataggccga ttgaacgaat gcggggtaga
     5941 gacccgcaag cgggatgatg cacactacca gcgccgccag caaagtaata acggcgggtg
     6001 cggtcaattc gctggtatgg atcccgatat ccaaccaaac cgcgagtctg gggaccagga
     6061 ctgcggctaa aatcgcccct atgatgacgg cgacgagaac ctgcaaggcg gtttccccaa
     6121 tgaattgccc gatcagttgc ggacgccttg ctccgaggac cttacggaca cccacttccc
     6181 tggcgcgggt ggcggattgc ccggttgcca ggttgatgaa attgatacag gcggtgatga
     6241 tgatgaatag cgctatgccg gccagtgccc aataggtctg ttt
//
LOCUS       DASYSV010000138        11672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_26678, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000138 DASYSV010000000
VERSION     DASYSV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11672
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26678"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VGQ51_04345"
     CDS             <1..869
                     /locus_tag="VGQ51_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9035820.1"
                     /translation="GTAKSEERMYEMLRVDDDYVKTYGLRIIAGRDFDKSRPLDSFGV
                     ILNESAVRQFGFASPEQAIGGRVWLETVDKHPSEVIGVIKDYHQQSLQKEFTPIILCM
                     DPALGWVPTDYFSVKINSGRMRDKVAELKNVWDRFFPESSFDSFFLEESYNRQYQQEV
                     QFGRNFMIFASLAIFVACMGLYGLTAYSTARRNKEIGVRKVLGASVRNIVTLLTRDVI
                     RLILFCSLIAMPAALFLIDRWLRGYAFRVKLVLWQFILPIAALVLIALLTTVWLTVRA
                     ALANPTTSLKDE"
     gene            943..2481
                     /locus_tag="VGQ51_04350"
     CDS             943..2481
                     /locus_tag="VGQ51_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9035821.1"
                     /translation="MKYLLAAGCLLLASLSLRAQLDNSFSGNGKVLAKIGSFTTAGSD
                     MLIQPDQNIVVAGYYDDGSTTGFAVMRFLPNGTLDPNFGSGGKVSIAFANYIFGSVKV
                     GIQTTQKLVLMGEFGLPNEGREAVLVRLAADGSLDLGFSPTGYLVIPAPIQAYAMAIQ
                     ADDKILVGGNGSGNGVDVARYSADGVLDNTFGTNGLATLTGGPINAMTVDGAGRIITV
                     GGGGATAFTQVSCFTHTGFPDPQFGVGGVTPVSVGNSPVFLDAVSMQTDGYILAAGQY
                     LPSGTNTKYRFMIVRLRPDGSLDPGFSGNGKQNVAFPSASAFATAVLQDWQGFIYAAG
                     IVEPTNGAVHTFGLCRLTVNGALDTRFGTIGVLTTGWSGQSANATAFAQQQNGKLVVA
                     GYAGLYDAVARYNNPFVLSPPPFVTSSDPGSISQLGSGLAPTGLRVFPNPVRSLLHVQ
                     GLDAKSSTLLLVRDAAGNALMSVKLEQQSEYSLDVHRLEPGTYFLELTAEGGRKSFPF
                     VKAP"
     gene            complement(2478..11174)
                     /locus_tag="VGQ51_04355"
     CDS             complement(2478..11174)
                     /locus_tag="VGQ51_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEV9035822.1"
                     /translation="MKVTASTVFDILSRLRSYFQKAANPWAYGDEEPFRSELYSSDQL
                     NNHAKAIAAKHLLQTTHPVDRLLNRLADNENTLLEVRDLLVGNIKSGKVITPGAEWLL
                     DNFYLIEEQVVLARKHLPKGYSEALPALATGNSAGLPRVYDIVLEIISHSDGRVDAHN
                     LGSFVAAYQTVQALTLGELWAIPIMLRLAVIENLRRVCGKIALDMIDLNLADYWADKM
                     IDTIKNETGDLILTIADMVRSKPPLSSPFVAGFIRNLQGKGPALALPLSWLEQQLAGV
                     GTNSNVLVRLENQKQAADQVSVRNSIGTLRFIGTTDWKEFVETLSLVEQILVKDPAGT
                     YSRMDFATRDRYRHVVESIAKGSRLPETEVAQKVLTLAGNHNDTHPSGKKEQHVGYYL
                     IGKGLKQTAQALGKQNGPTRRLLRKATKIPLFLYLCSIVLLTGLITAAMFYLPYSQGI
                     RNKYLLSVIILLVISASAQLAIALVNWLSTLLVKPNLLPRMDFSMGIPSEYRTLVIVP
                     TLLSSPAYIESLMEGLEIRFLANKEDHLHFGLLTDFVDADVEKLEGDQELIDLASAGI
                     DALNKKYRQTENDIFFLFHRSRLWNAGERKWMGYERKRGKITALNALLRRGGEHEFSL
                     IRGDYRILYDVKYVITLDSDTQLPRESAWKLVATMAHPLNQPEYNARKKCVTAGYGVL
                     QPRVESSIPLTATSLYLNMQGSDSGIDPYTRVSSDVYQDLFDEGSFIGKGIYDVDIFE
                     LTFRDLFQENRILSHDLLEGCYVRSGLVSDVFLYEENPSRYESDLRRHHRWIRGDWQI
                     AGWMLPFVTTGTGRVIRNKLSALSRWKIIDNLRRSLLPLSLFSILLLGWFVLPFPWFW
                     TLAVFTIILLPQMVAISWQLLHKPADITVKAHLSEIAGNLKISLIRFVFAIAILPFEA
                     FQYADAILRTGWRMLVTRRRLMEWTPFASHSGNRRGSLWSTYQFMPIGPLLALGCAAF
                     LLTNLQDLLVASPILFLWLLSPAVVWRLSKPETEPSPKLNTEELSFLHKTARKTWAFF
                     EQFVTADDNWLPPDNFQEYEGPVIAHRTSPTNMGLSLLANLAAYDFAYLSGTELMNRC
                     ALTLKTMNKLERYKGHFCNWYDTRSLSTLHPRYVSTVDSGNLVGHLLTLRQGLLAIPG
                     QPIFTDKVLEGFNTTVTIISGESKGRFDQIIGQLKSPLQQTLPDGGSLVTINRLLDEL
                     AGLVDRIRLPAEEADTELSRWVGRLSHQVSDHREQLRQLIPWIDLLPIPERFARLAPM
                     DRIPTLRSLQAMRDTCLKVLTEYEGQTQIAEDKKWLGRMRTSLDEAETLAISRIGQLE
                     QLAEQCEEFSEIEYDFLLEPSTGLLRIGFNLEEQRKDNGYYDLLASEVRLGIFAGIAQ
                     GKLLQESWFALGRLLADSGEDPILLSWSGSMFEYLMPRLVMPSYEKTLLAQTSKAMVR
                     RQIEYATQQGKPWGISESAYNLVDANLNYQYRAFGVPGLGLTRGLEENFVIAPYACMM
                     ALMVLPEDAVSNLQVLSAKGLEGEYGFREAVDYTASRVPRGKNSVIIRSYMVHHQGMG
                     LLSLAYVLLDKPMQQRFVSDLRFQATLLLLQERIPRTTLFYAHTEDMVDSHSSTPDTQ
                     IRVINTPFTRIPEIQLLSNGRYQVMVANSGAGYSRWKDLSVTRWREDPTKDDRGIFCY
                     IKDVTSGNFWSNTYEPTLRAGKKYEVIFSQAHVEFHRHDHGIDTNTEIVISPEDDMEM
                     RRVKITNRSAFSRVLEITSYAEVVMATQAADEAHPAFSNLFVETEILPEYSAVVCTRR
                     PRSEEETPPWMFHLMSVQGATVEATSYETARLPFIGRGRSLIHPQAMDMESLSGSQGA
                     VLDPIVAIRYRISIKPNHTATIDLIYGISETREACEALMHKYRDEHLRNRAFELSWTH
                     NQVLLRQINATEADAQLYDQLASSLIYPNPALRGEATVILNNFRGQSGLWSHSVSGDI
                     PIVLLHMSDQENLELARQMIQAHTYWRLKGLTVDLVIWNDDHGSYRQFLQDQIQGLVN
                     TDAGNQPYQKPGKIFVRSADQISPEDRILFESVARVVIYDHKGTLSEQVNHIYMGNTP
                     LPFLQSSPVQTEEPSDSLALPDNLLFDNGTGGFTPDGKEYKILTERKRTTPAPWVNVI
                     ANHAIGTVLSESGSGYTWCVNAHEYRLTPWSNDPVIDAGGEAFYLRDEESGYFWSPVP
                     FPVRGPTPYLTTHGFGYSRFQHVEQGIATEMCVFVDSDLAVKWTVLKIKNQSGRRRKL
                     SATGYLEIVLGDVSSRTGMHILSQQDTDSGALLFFNRYNPAFSDRVNFFKVDEAGPPA
                     FTADRLEFIGRNRNLQDPQAMHRKRLSGRTGAGIDPCAALQVAFELSEGAEKEMVFVL
                     GSAPNAREARELIGKLSGPRTAQLSLSRVTEYWKEILGSVQITTPDDALNIFGNGWLL
                     YQTTSSRLFGRSGFYQSGGAFGFRDQLQDVLALLHTRPDLAREQILLNASRQFVEGDV
                     QHWWHPPEGRGVRTHCSDDMLWLPYVVARYIAVTGDTDLLNSTVGYLESRLLYPEEDS
                     LYDLPASGNFSGTLFEHCVRAIKHALRFGQHGLPLIGTGDWNDGMDKVGNKGTGESVW
                     LAFFLYDILRRFSNTANSYGDHFFAEICATEADSLQSRIETAGWDGAWYKRAYFDDGT
                     PLGSAENKECRIDAVSQSWAVLSGAASDKRAVMAMSSLEEKLVRKDLKLIQLLDPPFN
                     STELNPGYIKGYVPGVRENGGQYTHAAIWSLMAFAMLGQREKVWQLLEMIHPINHSDD
                     DASVRVYKVEPYVMVADIYANESHKGMGGWTWYTGSAGWMYQFILGSLIGLDRRSDSL
                     SFKPCFPSNWPSIQLTYRYGRSEYRITVFQLSDDSASRWKMDDLQGSGNTIKLTDDGQ
                     IHLVEMHIRNGH"
     gene            complement(11415..>11672)
                     /locus_tag="VGQ51_04360"
     CDS             complement(11415..>11672)
                     /locus_tag="VGQ51_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV9035823.1"
                     /translation="FFAPYGEITSAKVIMDKITNQSRGFGFIEMPDEAAAKKAITELD
                     GASVENRTIKVNEARPKEARPARNDNSRNFNNANSYNKNRY"
BASE COUNT         2594 a         2983 c         3202 g         2893 t
ORIGIN      
        1 atggcacggc caaatcggag gagcggatgt atgaaatgct gagagtggat gatgattatg
       61 tcaagacgta cggactccgg atcatagccg ggcgggactt cgacaaaagc cgcccattgg
      121 actctttcgg cgtcatcctg aatgaatcgg ccgtaagaca attcggtttt gcttcccctg
      181 aacaggccat aggagggcgg gtatggttag aaacggtgga caagcatccg agtgaggtta
      241 tcggggtgat aaaagattat catcagcaat cgcttcaaaa ggaattcacg cctattattt
      301 tgtgtatgga ccctgccctg ggatgggttc ctacggatta tttctccgtt aagatcaatt
      361 cggggcgtat gcgcgacaag gtggccgaat tgaaaaatgt ctgggaccgg tttttcccgg
      421 aatcatcttt tgattccttt ttcctggagg aatcttataa ccggcaatat cagcaggagg
      481 tgcaatttgg acgcaacttc atgatatttg cctcacttgc catatttgtc gcctgtatgg
      541 gattgtatgg gctaaccgcc tattccaccg cccgtcggaa taaggagata ggggttcgta
      601 aagtattggg cgcatccgtt cgaaatatcg taaccctgct cacccgggat gtgatccggc
      661 tgatcctttt ttgcagcctg atcgctatgc ctgcagcgct tttcctgatc gatcgatggt
      721 taagaggcta tgcattcaga gtgaaattgg tcttgtggca atttattctt cctatagccg
      781 cgctcgtgct gatagcgctg ttaacgacag tctggctcac cgtgcgggct gctctggcca
      841 atccgaccac ttcgttaaag gatgaatgag ctttcttcac tcccccgatt acggtattga
      901 ggtcgggatt gtgcacgcta tcttgcgggc aaaacctgcg ttatgaaata tctgctggcc
      961 gcgggctgtc tgttgctcgc ttcactatca ctccgcgcac aactggataa tagctttagt
     1021 ggaaatggga aagtgctggc gaagatcggc agttttacta ctgccgggtc cgatatgctt
     1081 atccagcccg accagaacat cgtggttgcc ggatattatg atgatgggtc gacgacgggg
     1141 tttgccgtca tgcggttctt gccgaacggc acgctggatc cgaatttcgg atcgggcgga
     1201 aaagtttcga tcgcctttgc caattatata ttcgggtcgg tgaaggttgg gatccagacg
     1261 acccaaaagc tcgtgttgat gggggagttt ggtttgccca acgaaggacg cgaggccgta
     1321 ttggtgcggc tggcggcgga cggatcgctg gatctcggtt ttagtcccac cggttacctg
     1381 gtcattccgg cacccataca ggcctatgct atggccatac aagccgatga taagatactt
     1441 gttggcggca acggaagcgg aaatggggtc gacgtcgccc ggtacagtgc ggatggagtg
     1501 ctggacaata ctttcgggac gaatggcctg gccactttga ccggcggacc gatcaatgcc
     1561 atgaccgtcg atggagcggg gcgcattatt accgtcggcg gcggtggagc tacggccttt
     1621 acacaggtat cctgtttcac tcataccggc ttccccgatc ctcaattcgg tgtcgggggt
     1681 gtaacgccgg tgagtgttgg caatagcccg gtcttcctcg atgcagtaag catgcagacc
     1741 gatggctata ttcttgccgc agggcaatac ttgccctcag gcacgaatac caaatatcgt
     1801 ttcatgatcg ttcgtttgcg gcccgatgga tcgttagacc cgggtttctc ggggaatgga
     1861 aagcagaacg tcgcctttcc ttcggccagc gctttcgcaa cggcagtttt gcaggattgg
     1921 cagggattca tctacgcggc gggtatagtt gagccaacaa acggcgcagt ccacacattc
     1981 gggttatgcc ggttgaccgt gaatggggct ttggataccc gatttggtac aatcggggtc
     2041 cttacgacgg gatggagcgg tcagtcggct aatgcgacgg cgttcgcaca acaacagaac
     2101 gggaaacttg tcgtggcggg ttatgccggc ctgtatgatg ctgtggcccg atacaataat
     2161 ccttttgtcc tttctcctcc accgtttgtg acctcgtcgg atccgggttc cattagtcag
     2221 ctgggatcgg gactggcgcc gacagggcta agggtatttc cgaatccggt gcgctccctt
     2281 ttgcatgtgc agggattgga tgcaaagagt tccaccttgt tgttggtgag ggatgcagcc
     2341 ggaaacgcgc tgatgagcgt gaagctggag cagcagagcg agtattcgct ggatgtgcat
     2401 cggttggagc cggggacgta ttttctcgag ttgacagcag agggcgggcg aaagagtttt
     2461 cctttcgtga aggcgcctta gtgtccatta cgaatatgca tttccacgag gtggatctgt
     2521 ccgtcatctg tgagttttat cgtgttcccg gaaccttgca ggtcgtccat tttccaccgg
     2581 gatgcagaat catcggacag ctggaaaacg gtgatccggt attcggatct gccatagcga
     2641 tacgtcagtt gaatggaagg ccagttcgac ggaaagcaag gcttgaaact taatgaatcc
     2701 gaccgtcgat ccaatccgat caacgagcca aggataaatt gatacatcca gccggcggag
     2761 ccagtgtacc aggtccagcc gcccatacct ttatgcgact cgtttgcata aatatccgcc
     2821 accattacat aaggttcgac tttgtatacc cggacggacg catcatcatc cgaatggttg
     2881 atgggatgga tcatttccag cagttgccag accttttccc tttgacccaa catagcgaat
     2941 gccattaagg accagatggc ggcatgtgta tactggccac cgttctcccg gacgccaggt
     3001 acatagcctt tgatataccc ggggttcaat tcggttgaat tgaaaggtgg atccagcagc
     3061 tggatcaatt tcaggtcttt tctgacgagc ttttcttcga gcgaggacat ggccatgacg
     3121 gcccttttgt cgctcgcagc cccggacagg accgcccagc tctgcgaaac ggcgtcaatc
     3181 cggcattctt tattttctgc ggaacccaaa ggcgttccgt catcgaaata cgcccgttta
     3241 taccacgcgc catcccagcc ggccgtttcg atcctggact gtaagctgtc ggcttccgtc
     3301 gcgcagatct cggcaaagaa gtgatctccg taggaatttg ccgtattgct aaaccgtctc
     3361 agtatatcat acaggaaaaa agcgagccag acgctttcgc ccgtgccttt attgcctact
     3421 ttgtccattc cgtcattcca gtcgcccgtg ccgatgagtg gcaggccatg ttgaccaaag
     3481 cgtaaagcat gtttgatcgc ccgcacgcag tgttcgaaca atgtcccgct gaagttgccg
     3541 cttgccggca aatcatagag ggagtcttct tccgggtata gcagcctgct ttccagatag
     3601 ccgacggtgg agttcagcag gtccgtgtcg ccggtgaccg cgatatatcg ggcgacgaca
     3661 tagggtagcc aaagcatatc gtcggaacag tgggttcgga cacctcttcc ttcgggcgga
     3721 tgccaccagt gctgcacatc gccttccacg aactgcctgg atgcgttcag gaggatttgt
     3781 tcgcgggcca gatcaggtct ggtatggagc aaggccagca cgtcctgcag ctgatccctg
     3841 aatccgaaag cgccgcctga ttgataaaaa ccgctccgac cgaacaacct ggaggaagtt
     3901 gtttgataaa gaagccagcc attgccgaat atattcaggg cgtcgtcggg ggtggtgatc
     3961 tgtaccgacc ctaaaatctc tttccagtat tcagtgaccc ggctcagtga aagttgggcg
     4021 gtgcgcgggc cggaaagttt cccgatcaac tcgcgggctt cccgggcatt cggtgcgctt
     4081 cccaggacaa ataccatttc tttttcagcg ccttcggata gttcgaatgc cacctgcaag
     4141 gcagcacagg gatcaatacc ggcaccggtt ctgccggaca gccttttgcg gtgcattgct
     4201 tgtgggtcct gcaggttcct gttcctgccg ataaattcaa gccggtcggc ggtgaaggcc
     4261 ggaggtccgg cttcgtcgac cttaaaaaaa ttgacccggt cggaaaatgc agggttgtac
     4321 ctgttgaaga agagcagcgc cccgctatcg gtgtcctgtt gcgaaagaat atgcatacca
     4381 gtcctgctcg aaacatctcc caggacgatt tccaggtacc cggtagcgga gagcttgcgt
     4441 cgccgtccgg actgattttt tatcttcaac accgtccact taacagccag atcgctgtca
     4501 acaaaaacac acatttccgt agcgatgcct tgttcgacat gttgaaatcg gctatagccg
     4561 aatccatgcg tggttaaata aggggtaggt ccgcgaaccg ggaaagggac cggggaccaa
     4621 aaatagccgg attcctcatc ccgcagatag aatgcttccc cgcccgcatc gatgacgggg
     4681 tcattgctcc agggtgtcag ccggtattca tgcgcgttaa cgcaccatgt gtagccggag
     4741 ccgctttccg aaaggacggt accgatagca tggttggcga ttacattgac ccagggagcg
     4801 ggtgtcgttc tctttctttc cgtcagtatc ttatattctt tgccatccgg ggtgaaaccg
     4861 cccgtgccat tgtcaaaaag aagattgtcc ggcagtgcga gcgaatcgga tggctcttcc
     4921 gtctgcacgg ggcttgattg aaggaagggc aaaggcgtat tccccatgta aatgtggttc
     4981 acctgttctg aaagtgtgcc tttatggtca taaatgacga ccctcgcgac gctttcaaaa
     5041 aggatacggt cttcgggcga gatctgatct gcggatcgga cgaatatttt gccgggtttt
     5101 tgataaggct gatttccggc atcggtattg accaggccct gtatctggtc ctgcaggaat
     5161 tggcggtagg acccatggtc atcattccag atcacgaggt cgacggtcag gcctttcagg
     5221 cgccagtagg tatgcgcctg tatcatttgt cgtgcgagct ccaggttttc ctgatcactc
     5281 atatgcagca atacgatcgg gatgtcaccg gacaccgaat ggctccaaag acccgattgg
     5341 cctctgaagt tattgaggat cacagttgcc tcacctcgta aggcaggatt gggatagatg
     5401 agggaggaag ccaactgatc gtatagctgg gcgtcggctt ccgttgcgtt gatctggcgc
     5461 agcagtacct ggttatgggt ccaggaaagc tcaaaggcgc ggtttctcaa atgttcatcc
     5521 cggtatttgt gcatcaatgc ttcacatgct tcccttgttt cgctgatccc atagatcaga
     5581 tcgatggtgg cggtatgatt tggttttatc gaaatgcggt agcggattgc cacgatcgga
     5641 tcaagtactg ctccctggct gccggacaag gactccatgt ccatcgcctg cgggtggatc
     5701 agcgatctac cccggccgat aaatggcagc cgtgcggtct cataggaggt ggcttccacg
     5761 gtggcgccct gaacactcat caggtgaaac atccagggtg gcgtttcctc ttctgagcgc
     5821 ggacggcgtg tgcaaaccac cgctgaatat tcgggaagga tctccgtctc cacgaagagg
     5881 ttgctaaaag ccggatgcgc ctcatccgcg gcctgggttg ccatgaccac ctcggcatag
     5941 cttgttatct ccagcactct tgaaaatgcg gatctattgg tgatctttac ccgacgcatt
     6001 tccatatcat cttccggtga gatgactatt tccgtgttgg tatcgatgcc atgatcgtgt
     6061 ctgtggaatt caacatgggc ctgtgaaaat attacctcgt attttttgcc ggcgcgcaag
     6121 gtgggttcat atgtatttga ccaaaagttg ccgctggtca cgtctttaat ataacagaat
     6181 atgccccggt cgtctttggt agggtcttct ctccagcggg tgaccgaaag gtctttccac
     6241 cggctgtatc ccgctccgga attggcgacc atgacctgat agcgaccatt gctcaagagt
     6301 tggatttccg gtatcctcgt aaaaggggta ttgatgaccc ggatctgggt gtcgggcgta
     6361 gacgagtggg agtctaccat gtcctccgta tgagcgtaaa acagcgtggt gcgcgggatc
     6421 cgttcctgta aaagcagcag ggttgcctga aaacggagat ccgatacgaa ccgttgctgc
     6481 atcggcttat ccaacaggac ataggccagc gatagcaatc ccatgccctg atggtggacc
     6541 atataagacc ggataatgac cgaatttttg cccctgggca cccgggaagc tgtataatcc
     6601 accgcctcac gaaagccata ttcgccttcc agacctttcg ctgacaagac ctgcagattg
     6661 gaaacggcgt cttcgggcaa aaccatcaat gccatcatgc aggcataggg tgcgatgaca
     6721 aaattttctt ccagcccccg ggttagcccc agtccgggta cgccgaatgc ccgatactga
     6781 taattcagat tggcgtccac caggttgtag gcggattccg agatgcccca gggtttgccc
     6841 tgctgcgtcg catactcgat ctgccgtctg accatcgctt tactggtttg tgccagaagc
     6901 gttttctcgt aggaaggcat caccagccgg ggcatcagat attcaaacat cgaaccgctc
     6961 caggaaagta agatagggtc ttcaccggag tcggcgagca aacgtccgag ggcgaaccag
     7021 ctttcctgca ggagcttacc ctgggcgatg cccgcaaaaa ttcccagcct tacctcggag
     7081 gccaataaat cgtagtatcc gttgtctttt cgctgttcct cgagattgaa gccgatcctg
     7141 agaagcccgg tcgacggctc gagcagaaaa tcgtattcta tttcactaaa ctcttcgcat
     7201 tgttccgcca actgctccag ctgtccgatc ctggatatcg ccagtgtttc ggcttcgtca
     7261 agcgaggtgc gcatccggcc gagccatttc ttatcttccg caatttgcgt ttgtccttcg
     7321 tattcagtta gcaccttcag gcaggtgtcc cgcatggcct ggagagatcg caacgttggt
     7381 atccgatcca tgggcgccag tcgcgcaaag cgttcgggaa tgggtaacag atctatccag
     7441 ggaatcagtt gacgaagctg ttcccggtga tcgcttacct gatgggaaag cctgccgacc
     7501 catctcgata gttcggtatc ggcctcttct gccggaaggc gtatccgatc gacgagaccg
     7561 gccagttcgt caagcagtcg gtttatggtg accagcgatc cgccatccgg aagggtttgt
     7621 tgcaacgggg actttaattg accaatgatt tggtcgaaac gtcctttgga ttcgccggaa
     7681 atgatcgtta ccgtcgtgtt gaatccttcc agcactttgt cggtgaatat gggctgtcct
     7741 ggtattgcta acagtccctg acgcaacgtg agcagatgtc cgacgaggtt cccgctgtcg
     7801 acggtcgata catacctggg gtgcaaggtc gatagggatc gggtatcgta ccaattgcaa
     7861 aaatgccctt tgtagcgctc cagcttgttc atcgtcttaa gggtgagggc gcatcggttc
     7921 atgagttcag tgccggaaag gtaggcaaaa tcgtatgcgg caagattggc cagcagcgac
     7981 agtcccatgt tggttggcga tgtccggtga gcgataaccg gtccttcgta ctcctggaag
     8041 ttgtcgggtg gcaaccaatt gtcatcggct gttacgaact gttcgaagaa cgcccaggtc
     8101 ttgcgggcgg tcttatgcaa gaatgacaac tcttcagtgt tcaatttcgg actgggctcg
     8161 gtctccggtt tgctcaggcg ccataccacc gccggcgaca gaagccataa aaaaagaatg
     8221 ggactggcga ccagcagatc ctgtagatta gtcagcagga aggctgcaca gccaagggcc
     8281 aggagcggcc cgatcggcat gaattgataa gtggaccaga gagagccacg gcggttcccg
     8341 gagtgactgg caaagggcgt ccattccatt agcctccgcc gggtaaccaa cattcgccag
     8401 cccgttcgca gaatagcgtc cgcgtactgg aacgcttcaa atggaaggat ggcaatagcg
     8461 aagacaaacc ggatcagcga aattttcaaa ttaccggcta tttcggacag gtgcgcttta
     8521 accgtaatat cagcgggttt atgtaatagt tgccagctaa tagcgaccat ctgcgggagc
     8581 aggatgatcg taaagaccgc caacgtccaa aaccagggaa aagggagaac gaaccatcct
     8641 aacaaaagaa tggagaatag ggataggggt agaaggcttc gccgaaggtt gtcgattatc
     8701 ttccacctcg acaatgccga aagtttgttc ctgatcacgc gtccggtgcc ggtcgtgaca
     8761 aagggcaaca tccagcccgc gatctgccag tcgcccctga tccagcggtg atgacgcctg
     8821 aggtccgatt catatcggga cgggttttct tcatagagga aaacatcgct gaccaatccg
     8881 gaccgcacat aacagccttc cagcaaatcg tgactcagga tccggttttc ctgaaaaagg
     8941 tccctgaaag tcagctcaaa aatgtctaca tcatagatcc cttttccgat gaaagatcct
     9001 tcgtcaaaaa ggtcctggta tacgtcggag gagacgcggg tataagggtc gatacccgaa
     9061 tcgctgcctt gcatattcag atagagagaa gtcgcggtta aagggatgga cgactccacc
     9121 ctcggctgta acacgccgta tcctgccgta acgcattttt tccgcgcatt gtattcgggc
     9181 tggttcagcg gatgtgccat cgtcgctacc agcttccatg cggattctct cggaagctgg
     9241 gtgtcggaat ccagggtgat cacgtatttt acgtcataca gaatgcggta gtctcccctg
     9301 atcagggaaa attcatgttc ccccccgcgc ctcagcaggg cattaagcgc tgttatcttc
     9361 ccccgtttgc gttcgtagcc catccatttt cgttctccgg cattccaaag cctggagcga
     9421 tggaacagaa agaatatatc gttctccgtt tgccggtatt tcttattcaa cgcgtcgatc
     9481 ccggcgcttg ccagatcgat caattcctga tctccttcaa gcttttcgac atcggcgtct
     9541 acaaaatcgg tcagcaatcc aaaatgcagg tggtcttctt tgttggccag gaaacgtatt
     9601 tctaatcctt ccatcagcga ttcgatatac gccgggctgg aaagcagggt gggtacgatg
     9661 accagggttc gatattcgga aggaattccc atggaaaaat ccatgcgtgg caataagtta
     9721 ggcttgacca gcagcgtgga gagccagttg accagggcaa tggccagctg tgcggacgct
     9781 gagatcacca gcaggattat caccgatagc agatatttgt tgcgaatgcc ctgactgtag
     9841 ggaagataga acatcgcagc ggttatcaac ccggttagca aaacgataga gcacaggtaa
     9901 aggaaaagtg ggatcttcgt tgctttccgg agcaggcgcc gggtggggcc attttgtttt
     9961 cccagcgctt gtgctgtctg ctttagtcct ttaccgatca gatagtaacc tacgtgttgc
    10021 tcttttttac cggaaggatg agtgtcattg tggtttccgg caagggtgag cactttttgt
    10081 gcgacctccg tctccggcaa cctggaacct ttggcgatgg attccacaac gtgccggtat
    10141 cggtcccggg tagcgaaatc catacgggaa taggtgccgg caggatcctt taccaatatt
    10201 tgttctacga ggctgagcgt ctcgacaaat tccttccagt ccgtcgttcc gataaatcgc
    10261 aaagttccga tgctgttacg gaccgaaacc tggtcggcag cttgtttttg gttttccagc
    10321 cggaccagga cattgctgtt ggtacccact cctgccaatt gttgttccag ccaactgagc
    10381 ggcaaggcca atgcgggtcc ctttccttgc aggtttcgga taaaaccagc gacgaatggg
    10441 ctgctcaacg gaggttttga ccgtaccatg tcggcgatcg taaggatcag atctcccgtc
    10501 tcgttcttta tggtatcgat cattttgtcg gcccaataat cggcaaggtt gagatcgatc
    10561 atatcgagcg ctatctttcc gcaaacccgc cgcaggtttt cgataaccgc caatcgcagc
    10621 atgatgggta tggcccacaa ctcgcccagc gtaagtgcct ggaccgtctg ataggcggct
    10681 acaaaactgc cgaggttgtg ggcatccacc cggccgtcgc tatgagaaat aatctccagt
    10741 acgatatcgt agacccgcgg gaggcccgcg gaattgccgg tcgccagtgc ggggagcgcc
    10801 tcactatatc cctttgggag gtgtttccgg gccagcacta cctgctcctc gatgaggtaa
    10861 aaattgtcga gcagccattc tgctccggga gtaatgactt tccccgactt gatgttcccg
    10921 accagtaaat cccgcacttc cagcagcgtg ttttcattgt cggcaagtct gttcaacagt
    10981 cgatcaacgg gatgggtcgt ttgcagaagg tgctttgcgg caattgcttt ggcatggttg
    11041 ttcagctggt ctgaactgta cagttcggag cgaaagggct cctcgtcgcc ataagcccac
    11101 ggattcgcgg ccttctggaa ataagagcgt agacgtgaca agatgtcaaa aaccgtactg
    11161 gctgtgactt tcataataga aatatttgag cgcggcgatt gacacaatat tgcctaaaat
    11221 acgcttttcc aatggtttgt ttactgctga tgttgcaaaa cgaatgcccg ggcgttttct
    11281 cttcagcgcc agcggtttgg ctcatacttt tcgataagga ttcgggagca ggtgatgaat
    11341 tcggaccctc ggctgccgtc cgataataaa aaaagccccg gttcgccggg gctttaattc
    11401 aatttaaaca aatatcaata cctgttttta ttgtagctgt tggcgttgtt aaaattgcgg
    11461 ctgttatcat tgcgtgccgg tcgagcctct ttaggccttg cttcattgac cttgatggtc
    11521 ctgttctcga cggatgcccc atccagttcg gtgatcgcct tcttcgcagc ggcttcatca
    11581 ggcatttcaa taaaaccgaa tccacgggat tggttcgtta ttttgtccat tatgactttc
    11641 gcggaggtaa tttcgccata aggagcaaaa aa
//
LOCUS       DASYSV010000139         4246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1433648, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000139 DASYSV010000000
VERSION     DASYSV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433648"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VGQ51_04365"
     CDS             complement(<1..839)
                     /locus_tag="VGQ51_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEV9035824.1"
                     /translation="MDRIYFDNAATTPIDPQVLEAMMPYLTEKFGNPSSIYSYGRESR
                     LAMENARKSVAKILNAHPAEIFFTSGGTESSNTAITAAVRDLGCTHIITSPLEHHATL
                     HTVEHLHKRGEAALSFVKVLPNGHIDLEDLEQQLAASPEKAVVTLMHANNEIGNMLDI
                     QSVGELCKLYGAIFHSDTVQTVGHFPFDLRNTPVHFITGAGHKFHGPKGVGLLYINEN
                     VRIHPYIHGGSQERNMRAGTENVYGVVGFARALELATAAFEDDSASIKGLKVYMMEQL
                     KRQ"
     gene            complement(930..2315)
                     /gene="glmM"
                     /locus_tag="VGQ51_04370"
     CDS             complement(930..2315)
                     /gene="glmM"
                     /locus_tag="VGQ51_04370"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV9035825.1"
                     /translation="MALIKSISGIRGTIGGKPGENLSPLDVVKFTAAFGSRILQTGKE
                     KTIVIGRDARISGEMINALVVSTLRSLGLNVIDLGLSTTPTVELAVTGEKAAGGIILT
                     ASHNPKEWNALKLLNGNGEFISATEGAAILERVQKEDFVFASVDKLGEYRRSDVWLQR
                     HIDAIVKYPLVDVDAIRQKKYKIVVDVINSTGALFIPPLLKALGVGEVILLNEEINGR
                     FAHNPEPLPENLSGLSAAVVKTKADMGIAVDPDVDRLCFVSEDGSMFGEEYTLVAVAD
                     YVLGQRPGNTVSNMSSTKALKEITIRHGGEYHPSAVGEVNVVRMMKSVGAVIGGEGNG
                     GIIVPDLHYGRDALVGIGLFLSALSHHKNGIKSFRTKYPDYFISKNKIELRSGIDVPA
                     ILEKIKKKYKNHPVNTDDGLKIEFDNDWVHLRSSNTEPIIRIYAESSFETTANNIALR
                     LMQDIKEAIAP"
     gene            2492..3124
                     /locus_tag="VGQ51_04375"
     CDS             2492..3124
                     /locus_tag="VGQ51_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV9035826.1"
                     /translation="MVLIDAGPPHPVFLHKVLQGDYWLFSRINQGWTSPFLDTVFPFI
                     RQAECWFPFYLFLLMFITLNFGKKGWLWYLYLPMTAIIGELLSRYLIDDHLVFRLRPC
                     NNPLWADSLRFLADSCPAGSGFFSAEACDHFAIAFFLFRTLRHTSPWWRLVFAWAFLI
                     SYAQIYVGTHYPLDILLGGVLGSVIGIFSARLFRLQAGTLLLEPYKSSHA"
     gene            3117..>4246
                     /locus_tag="VGQ51_04380"
     CDS             3117..>4246
                     /locus_tag="VGQ51_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV9035827.1"
                     /translation="MLEILILLFLIFLNGLFVMAEIALVSARKSRLENLADNGDDRAK
                     SALRLSEKPELFLSVAQIGITLIAILTGFYSGEKFSADLTPYLEKIEFLRPYASNIST
                     ALILVIVTLLSIIFGELIPKRIGLLRAEQIARLVARPMRTLSRIAYPIVWLLNKVSNL
                     FFKFFSIRPSEDSAVTEDEIKAIINEGTEQGTIDEAEQEIIERVFHLGDRNITSLMTH
                     RNDIIWFDLNENEETIKEKIIREPHSVYPICDGEIDNIKGVVSIKDLYVTDDSVLFKD
                     IMTPALFVPENNTAYLVLEKFKTSRIHSCFIVDEYGSLLGMITLNDILEAIIGEMAQP
                     DITDYEIREREDGSYLVDAQIPFYDFLARFHKGDWLTDSDND"
BASE COUNT          954 a         1263 c          977 g         1052 t
ORIGIN      
        1 aactgcctct tgagttgttc catcatatat accttcaacc cctttataga tgcgctgtcg
       61 tcttcaaagg ccgcggtggc cagctccagc gcccgggcga aaccgacgac gccatagaca
      121 ttctccgtac ctgcgcgcat gttccgctcc tgcgatccgc cgtggatgta gggatgtata
      181 cgcacattct cgttgatata caaaagaccg acacctttcg gcccatggaa tttatgaccc
      241 gcgccggtaa tgaagtgaac cggcgtattg cggagatcaa acggaaaatg accgacggtt
      301 tgcaccgtat cggaatggaa aatagccccg tatagcttgc acagctcgcc caccgattga
      361 atatccagca tgttcccgat ctcattgttg gcatgcatca gcgtcaccac ggccttctcc
      421 ggactggccg ccagctgttg ttcgagatcc tcgaggtcga tatgaccgtt cggcaacacc
      481 ttcacgaagc taagcgccgc ttcaccgcgt ttgtggagat gttcgactgt atgcagtgtg
      541 gcatgatgtt ccagcggaga ggtaatgata tgggtacagc cgagatcgcg tacggcggct
      601 gtgatcgccg tattggagct ttccgtgcct cccgaggtga aaaagatctc cgcaggatgc
      661 gcattcagga tctttgcaac ggatttgcgg gcgttttcca tcgccagccg cgactccctg
      721 ccataagaat aaatagaaga aggattaccg aacttttccg tgagataagg catcatcgct
      781 tccaatacct ggggatcgat gggggtggta gcggcattgt cgaaatatat tctgtccata
      841 gcggactcaa aggtaaatga atttcgcaaa ccgaatgtcg gccccgcgac gttgcccccc
      901 aatggcccag gccaccttgc gacgcggatc tagggcgcga tcgcctcctt gatatcctgc
      961 atcagcctca acgcaatatt attggccgtc gtctcgaagc tgctctccgc atagatccgg
     1021 ataataggct ccgtattgga tgaccgcaaa tgcacccagt cgttatcgaa ctcgatcttc
     1081 aacccgtcat ccgtattcac cgggtgattc ttatatttct ttttaatttt ctccaatatc
     1141 gccggcacat cgatgcccga ccgaagctcg atcttattct tggagataaa ataatcggga
     1201 tatttcgtcc ggaaggattt aataccgttc ttgtgatgcg aaagagcgct gagaaaaaga
     1261 ccgatgccga ccagcgcatc ccgcccgtag tgcagatctg gaacgatgat acccccattc
     1321 ccttccccgc cgatcaccgc accgacggat ttcatcatcc tgaccacatt gacctctccc
     1381 accgccgagg gatgatactc gccgccatgc cggatcgtga tctccttcag cgccttggtg
     1441 ctgctcatat tgctgacggt attaccgggc cgctggccga gtacataatc ggccaccgca
     1501 accaatgtat actcctcccc gaacatgctc ccatcttcag agacgaaaca aagcctgtcc
     1561 acgtccggat ccactgctat tcccatatcg gccttcgtct tcaccaccgc cgcgctcaga
     1621 cccgaaagat tctcaggcaa cggttccgga ttatgggcaa accgcccgtt gatctcctcg
     1681 ttcaaaagga tgacctcgcc cacccccaaa gccttcaaca acggaggaat gaacaatgct
     1741 cccgtggaat tgatgacgtc tacgacgatc ttatacttct tttgccggat agcgtccacg
     1801 tcgaccagcg gatatttgac gatcgcgtcg atatgacgct ggagccagac atcgcttcgc
     1861 ctgtattcac ctaatttatc caccgaagca aagacaaaat cctccttctg cacccgctcc
     1921 aatatggcgg cgccttccgt ggcactgatg aactcaccgt tgccgttcaa taatttcaag
     1981 gcattccact ctttcggatt atgactcgca gtaaggatga tcccgccggc cgccttctcc
     2041 ccagtcaccg ccagttccac cgtcggcgtc gttgacaaac ccagatcgat gacattcaaa
     2101 cccaggctcc tcaaagtgct taccaccaga gcattgatca tttccccgct gatccgggcg
     2161 tcacgaccga tcacgatcgt tttttcctta cccgtctgca ggatccggct cccaaaagca
     2221 gcagtaaatt tgacaacatc caacggagaa aggttttcac cgggttttcc tcctatcgtt
     2281 cctctgatac ccgaaatgct tttgatcaac gccacaaata catgttttta ggtgtccaca
     2341 aaaataaggt ttcctgcaaa tttgcggtag gtgttttcct cctaattttc tcaaaagatg
     2401 gctccgtctg gcgcgcaatt tgcaagcatg cacctcccga tatgcgcagc cgatcgataa
     2461 tttattatat ttgataaatt tgtcgccgaa aatggtatta atcgacgcgg ggcctcctca
     2521 tccggtgttt ttgcacaagg tcttacaggg tgattactgg ctcttttcac gtatcaacca
     2581 gggctggacg tctccttttc tggatacggt gtttcccttt atccgccagg cggaatgctg
     2641 gttcccgttc tacctttttc tgctgatgtt catcaccctg aatttcggaa aaaaaggctg
     2701 gctctggtac ctctacctgc ctatgaccgc catcattgga gaacttctga gccgatacct
     2761 gatcgatgat cacctggtct ttcgtcttcg tccctgcaat aaccccctgt gggcggattc
     2821 cttgcgcttc ctcgccgatt cctgcccggc aggctccgga tttttctcgg cggaggcctg
     2881 cgatcatttc gcgatcgcat ttttcctttt ccgcactttg agacacacaa gcccctggtg
     2941 gcgattggta tttgcctggg ccttcctcat ctcttacgcg cagatatatg tcggcactca
     3001 ctacccgctg gatatcctcc tcggcggagt gctgggctcg gtcattggca tcttttccgc
     3061 ccggctgttc cggctccagg ccggtaccct ccttttagaa ccctataaat catcccatgc
     3121 ttgagatcct tattttattg ttcctcattt tcctgaacgg tctgttcgtc atggcggaga
     3181 tcgccctggt ctcggcccga aaaagccgtc tggagaacct ggcggacaat ggggacgatc
     3241 gggctaagtc cgcgctcagg ctctcggaaa aaccagaact gttcttatcc gtcgcccaga
     3301 taggcatcac cctgatcgcc atcctcaccg gtttttattc tggggaaaaa ttcagcgccg
     3361 atctgacccc ctacctcgag aagatcgaat tcttacgccc ctatgcctcc aatatctcca
     3421 ccgccctcat cctggtcatc gttaccctgc tatccatcat cttcggggag ctgatcccca
     3481 aacgcatcgg gctgcttcgc gccgaacaga tcgcccgcct ggtagcaaga cccatgcgta
     3541 ccctctcgcg gatcgcgtat cccatcgtct ggctgctcaa taaagtctcg aacctctttt
     3601 ttaaattctt ttctatacgt ccgtcggaag acagcgccgt cactgaggat gagatcaagg
     3661 ccattatcaa tgaaggcacc gagcagggca ctatcgatga ggccgaacag gagatcatcg
     3721 aacgcgtctt tcacctgggt gaccggaata tcacctctct gatgacccac cgcaacgaca
     3781 tcatctggtt cgacctcaac gaaaatgaag agaccatcaa ggaaaagatc atccgggagc
     3841 cgcattccgt ctatcccatc tgcgacggcg agatcgacaa tatcaaaggc gtggtctcca
     3901 tcaaggacct ctatgtgacg gatgacagcg tgctgtttaa agacatcatg acccccgccc
     3961 tgttcgttcc ggagaacaac acggcttacc tggtcctcga gaaattcaag acctcacgga
     4021 tacactcctg cttcatcgtc gatgaatatg gaagcctgct gggcatgatc accctcaacg
     4081 atatcctcga ggccatcatc ggtgaaatgg cccagccgga tatcactgac tatgagatcc
     4141 gcgaacgcga agacgggagc tacctagtgg acgcgcagat cccattttat gatttcctcg
     4201 cacgattcca taagggagac tggcttacgg acagcgataa cgattt
//
LOCUS       DASYSV010000140        18354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2075015, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000140 DASYSV010000000
VERSION     DASYSV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 18354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18354
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075015"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(864..2390)
                     /locus_tag="VGQ51_04385"
     CDS             complement(864..2390)
                     /locus_tag="VGQ51_04385"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9035828.1"
                     /translation="MNKNFFPGFLLLILVGTSCTKDLNQIPISSATTATFYKTPSDFI
                     QGVNAAYSDLRGYPDRLLNLSEIRSDNIYGVSVVVRDWDPINDFSSGIASNTYVEEGW
                     NTDFNGIFRANTALDQLSKNGSNVGSASLATRLQAESKFLRAFYYFDLVRYYGRLPIV
                     DHPMTTSEANNLKQSSVTDVYALIISDLQFAIANLPASYTGVDVGRATKYAAEGLLAQ
                     VYMARSGPAYSINGPGLALNEWALALPLINDIVGSGLFAFNPNYANIFSYSNQSPTST
                     GNKEAVFDVMYQTGISGTASTYGATYPWDLTPNGYFLSLKDTKSNGSLEIIPVSQDLV
                     ADYETGDLRLPATIYTAGYTNAGSTELRPFFKKYLDVTKIPTTSRFDWGINFMAIRYT
                     DILMMKAECILNGAPGNQTTVDSIVNLVRTRAGLASKTGVTLTQLFDERRKEFADEGF
                     RWFDLQRSGQVLTIMNDWISKEDVQHRINTVVANYVIYPVPQSQLDAAPGLYTQNPGY
                     "
     gene            complement(2411..5638)
                     /locus_tag="VGQ51_04390"
     CDS             complement(2411..5638)
                     /locus_tag="VGQ51_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9035829.1"
                     /translation="MKSNALLRRGLLLTFLLRLCMLSFSQQNFQVSGKVSDETGKPLT
                     NVTVSVKGSRTSTVSGADGSYQLTVPSGNVVLVFSYVGYEQQEIKLNSRHELSLSMKI
                     AGTSMSDVVVIGYGTQKRANVTGAVATLRSDAFDDRPITRVDQALVGQVAGVTVKQNT
                     GIPGKAFSIQVRGSGSISAGNEPLYVVDGMPISNNSSNTGNGSFVGGNPMDNINPNDI
                     ENVEVLKDAAAAAIYGSRASNGVVIITTRHGKNGKPVIGVSGYAGYNEAAKKLQMLNG
                     QQWIDRATEMINAAYVLKYGPAGATAGDDPATRAAKIGLAPGQVNVAYMLDPRWSTPG
                     HPGLEFKDWQSDIERKGPMQNAGLSASGGTDAVRYYFAGNYARQDGFIKGVDYKEYSA
                     RANVDVNASKILRLGVAVSPSYSITDDPGVEGKDNIFHQALSMSPVQEDSVGAYPNIG
                     KNAQYLWSNSTNGSLGKLTYNIGQTKRARVLGIIYGEVQLYKGLAFRSTLNVDNVNSN
                     TSTYVPYITQGTVTARTFNAATNPNLTAATSGTYSNYRRLTLVNENTLTYNAVIAEDH
                     SLNFLVGQAYNKDRLDQMGITSVGGFTSASIQTLNAAADIGFTGNNVSSQNVLISYFS
                     RLQYDYKQKYFLSASLRRDASSRFGANNQYANFPSVSLGWRIIQEKFMNRLGVVSDLK
                     LRGSYGVNGNNNIGDYDIPTLGSYGYVLGSPQGAAIGQAPNVIANPNLKWERSQTYDA
                     GIDFGLFNNRLTGSFDYYNKLNTQLLLNVPVPEVTGLQFALANAGSVRNIGQELELTS
                     RNMVGRFQWTTSINISHNGNKIVALYGNQNQIIIPNGFDVTDNIQRVGSPLNSIYVVK
                     MIGILTQDDINKKVAVYGTGETVGDPRYEDLNHDGVITEADKQIVGHPNPNYTYGMTN
                     VFRYKGFDLSVLIQGQSGGSIYSLLGRALTRTGQGFTDNAPEFYIHRWESPTNPGAGR
                     VSKAYSTFGFVANTDWLYSSDYIRVRNITFGYNLKQLIRTSVVQNARLYVSAENFFGH
                     DKYSGGLNPEAANTTISTNSAYPEAGDYGGLPLAKSLIVGLNINF"
     gene            5904..7532
                     /locus_tag="VGQ51_04395"
     CDS             5904..7532
                     /locus_tag="VGQ51_04395"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV9035830.1"
                     /translation="MRGLMMDYQLTVAAILRRVESLFAHKPIITRLPDRSIHRYTYRD
                     FAKRVKRLSVALQRLGVGEGDRVATLCWNHYQHLEVYYAAPCMGAVMHSLNLRLNPAD
                     LSYIVNHAEDKVIIVDQVLLPLFEQFRDKIKVASIIVIRQCEEPLPAGYLDYEDVIAK
                     GDAAQFRPFEGDENSAAFLSYTSGTTGKPKGVLYSHRSIVLHAMGSLISCAGFGITER
                     DVVLPIVPMFHASAWGLPFNCALAGAALVLPGPYLDAESLLQLFENEKVTITAGVPTV
                     MLSILSRLEAEPQKYKLFLRTIVIGGSAAPRSMVKTFDERYGIQLLHSWGMTEISPLG
                     STATPTSRLSAASKEVQYDYSMKQGLPVPLVEIRGRNESGLIPWNGVTMGELEVRGPW
                     VASAYYRDEGPSAKFTSDGWLKTGDIVTITEEGCIEIKDRSKDVIKSGGEWISSIALE
                     NALMGHPSILEAAVIAIPDSRWIERPFAYVVPKEGKTVSVEELCTFLVNKFPKFWLPD
                     GYAVVTAIPKTSVGKFLKSALREAYAREHPGDGV"
     gene            complement(7559..8854)
                     /locus_tag="VGQ51_04400"
     CDS             complement(7559..8854)
                     /locus_tag="VGQ51_04400"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV9035831.1"
                     /translation="MKRCLTILVILLASIANVFPQEKDRSVLTPGAPGSGGFSAARLA
                     RLDSGMSDWVKKKWINGSVAFIARRGKIVYYKAYGYNDLVKKAPLPKDDIFRIASQTK
                     AITSVAVMMLWEEGKFGLDDPVSMYIPSFAHQGVLASFNWKDTTYTTVPAKRPITIRD
                     LITHTSGLGYPAIGTPEANAIYAKDAMTGGVGVKQQTLADAMNRLGKLPLMFQPGEKW
                     MYGLGHDVLGYLVQIWSGMSLEEFFSKRIFRPLGMNDTYFNVPASKGSRLVNFFMEDS
                     SGIHRQDKTFGGALDMNFPLQHTDYFSGGGGLSSTISDYAIFLQMLLNGGEYNGVRLL
                     SRNTVRMMTTNQIGDLFINIGGEEYKWGLGVSIESEKGSRLMPSQAGSYGWGGVFSTV
                     YWVDPKEDMIVLLYMQMWGPHVADAQKQFKPLVYQAIDD"
     gene            8927..9667
                     /locus_tag="VGQ51_04405"
     CDS             8927..9667
                     /locus_tag="VGQ51_04405"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HEV9035832.1"
                     /translation="MRLQNKIAVITGGAAGIGLATAERFAKEGAKLVLWDVSDKGNEV
                     AQRLRKEGHDAIFKKINVTDQDEVQTAVGEARDHFGRIDVLINNAGITRDRTLLKMSK
                     NEWDDVIGVNLSGVFYCTQAVAPIMKEQNYGRIVSASSNVAIRGNFGQTNYVATKSAI
                     IGMTKVWAMELGRYGITVNCIAPGFIHTAMTDAMPEEVRKQSIPHIPLGKWGYPEDIA
                     NGYLYLASDEASFVSGICLTIDGGAARP"
     gene            9676..11217
                     /locus_tag="VGQ51_04410"
     CDS             9676..11217
                     /locus_tag="VGQ51_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV9035833.1"
                     /translation="MLAICLTTAAWPALAQKEVIYHIFQRSFFDANGDGIGDLRGLKE
                     KLGYLQQLGVTSILLTPLYKSVFYHNYFADDWYAIDPGYGSIKDFTGLARALHVRGMK
                     LYIDMETQYVTDRHPWWTSAVGNLRSPCRDYLLFEDSAHQKPAPIIWGITELKGYNGD
                     SRKVTTVNLHDPGVLAYNERLFSFFADPNGDGKFDDGVDGFRLDHTMDDLDGNPRLGD
                     LFTRFWAPLIDRLKKLNPRLTFVAEQANWADFGFDYFRSGKLDRVFAFRLAMAIQSFD
                     KSQLEKAADTTLRQLPPGKEQVVFIENHDLDRFASRIDNDAGKLRVAAVLNILLGGIP
                     SIYYGQELGMFGKGGKYGMTDANDIPRREAFEWYAADTGRGMALWYKGTGPWWDSTNL
                     IPHDGVSLEEERSDSGSLWVWYRRLLEVRKEFPVLQTGVYDSLHNGNPKVFTWRRRDA
                     RFTAYLAVNLSDQPQQTTIETERVVRDVFGNATVPSGQKRFTINLSPYGAGIWMTVDA
                     EASRK"
     gene            11281..12246
                     /locus_tag="VGQ51_04415"
     CDS             11281..12246
                     /locus_tag="VGQ51_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035834.1"
                     /translation="MKPIYLLLPIALLLRAASAQQPFASAQQQQPAAARQPAAASLAD
                     YGYNIPAGWTPTQYPDGIVLSAPVSNTAERCLLQIWPMRTPSGNLAQDAVTAFRQVFS
                     AYQPTESQYATRNSVIRGTSAQGWDYFMIKNAIRLSEGNYQVMWGFVFVAGLGNRVAV
                     ISGISKDPLVSSCFGLNLSDVWPKFFYSLRFKGWNSSLSPQQMMKRIAGSWIAVTATA
                     GDKWVFAANGRFASASAAQRYYSISSNEAISVTDAYFGDGSWSLDGQAIVLTKDNDKA
                     RPSKGWIRLEQESYDNGNNWAEHLYLLRTSTVDGKEYEMNYHRDK"
     gene            complement(12429..13688)
                     /locus_tag="VGQ51_04420"
     CDS             complement(12429..13688)
                     /locus_tag="VGQ51_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HEV9035835.1"
                     /translation="MSSRELRLIPLALLFAIACSKSNSPAPPPVNPGGPSKPDTIQGT
                     SAAIDPSNGAQIIRGFGGATVFLGALTTAEMNLLYGTQSGQLGLTVLRIRVDPGGPGN
                     WGVELGNAQKAIANGAIVMATPWSPPANFKSNGSTIGGVLDTASYAAYANYLDSFAVY
                     MSANGAPLYALSIQNEPDISVTYESCNWTAQQLFNFMKYNAPAIKNTRLIASESFHFD
                     PSYTDPILNDSTAASHLSIVGGHIYGAGLAPYPLAVSKGKEVWMTEHLDTSTDWTGAL
                     NTAKEISDCMGTAGFSVYNWWYLKRSYGPIDQNDNPTKRGYVMAQFAKFVRPGSQRIS
                     VTYSPSNNVYVTAYKHGTGLVIVVVNMGTTVVNQPFSVTGTSPSSFTPYVTSADKSLS
                     SDNPVNVGSGSFSYPLLPKSVTTFVSN"
     gene            complement(14113..17076)
                     /locus_tag="VGQ51_04425"
     CDS             complement(14113..17076)
                     /locus_tag="VGQ51_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9035836.1"
                     /translation="MRSVKEKQLDFLWGNTEMTHRIRQFDWSKTTLGPLSEWSPSLKN
                     VVGLMLANRFPMILWWGEDYIQLYNDPYIPITGLKHPQRSFGVPASEGWSEIWHILKP
                     LIDSPFNGGPATWMEDITLILNRNNFTEETHFTIAYSPVPDNEDSLGIGGVLATVNEI
                     TATVVGRRQMETLRDLGKNIAATLSEDEVYSKTLEVLANNPQDIPFASIYRIRKDEVE
                     DAPEAMQENILVAAQQRKIVVFPMAPETGGWAKGAWDVAPSFYVCVPVRQSGSRAPVA
                     VIVAGLNPYRQFDDSYHNFFQLIADQVSLGLNNAFAYEEERRRSQQLEELDKSKTIFF
                     SNISHEFRTPLTLILGTIEQLLKESALSVSDHDRLEIAHRNSIRLLRLVNTLLDFSRI
                     ESGRQKAAYTLTDIAGLTKNLASNFRSVIEKAGLEFVVGTDDEPGEPVYVDRDMWEKI
                     VFNLLSNAFKYTLKGSISVRLFFREHQVLLEVSDTGTGIPEKELPHMFERFHRVENSG
                     GRTIEGTGIGLSLIKELVHLHCGDIGIKSVEGEGTTVTVTLPTGKDHLPSGQVAGIPY
                     EEEHSGVYIGEASALFEHMPYQEPDQDGGDLKDTVLVVDDNSDMSNHIASILQKYYHV
                     VTASNGKEALKKIDTVMPTLVLSDIMMPEMDGIQLLKTLRQDKRTARLPVVLLTARAG
                     EESRMEGYETGADDYLVKPFAGNELLSRVRTQVSIARKRAEVEDRLEAEVARRTFQLK
                     QMNKELESFNYIASHDLQEPLRKIQTFILLLQERRDDPAAWKKYAGKINESSQRMSKL
                     IQSVLEYSRLSQVTASAASTDLNTILKNVKTDYELIIRESGAEISSDHLPTVEAIPFQ
                     MQQLFSNLISNGIKFAGKKPVIRISSEIVTGDKIGVLPDGPPKQTYAEIRFADNGIGF
                     EQQYTDKIFKLFQRLHPKSEFSGTGVGLSIVAKIVKNHRGYIMAESQKGEGAVFTVWL
                     PLKFEGGEEGWQVEE"
     gene            complement(17082..17483)
                     /locus_tag="VGQ51_04430"
     CDS             complement(17082..17483)
                     /locus_tag="VGQ51_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9035837.1"
                     /translation="MAKEPLVFLIEDDTDDQEFFVMAVENAFPTIKCICADTGIDALE
                     ILKADRAFVPDKIFVDINMPRMDGMECLMEIKKIHSLESVPVYMYSTSAEPAIVKSCI
                     RLGAAGFIKKEISISGLQRQLEEVMRNPKKE"
     gene            17743..>18354
                     /locus_tag="VGQ51_04435"
     CDS             17743..>18354
                     /locus_tag="VGQ51_04435"
                     /inference="COORDINATES: protein motif:HMM:NF036998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035838.1"
                     /translation="MRSFPLSAAYTDLYELTMGEVFFREGKQDTAVVFDYFFRKVPGG
                     GGYVLFAGLEDLLETLSALRFTDEDIAFLRDQGFHASYIDFLRGWEFKGMILSAREGE
                     IVFPGCPILRVEGGLFEAQLIETLLLNILNFESLIATKASRIKYVAGDRVVSDFGLRR
                     SHGPGGILATRAAFVGGATSTSNVYAAEKYHLPVTGTMAHSFVE"
BASE COUNT         4182 a         4985 c         4749 g         4438 t
ORIGIN      
        1 gctctctttt gcttttttcg gatccataat taatctctaa atgtaagaga tttttctgtc
       61 cttggaaatt gggccatctc acttcgccaa ccgtttatcg aaggtgagca agctaaattg
      121 caggacatcc aagcgttgct aactttcaat ggttttgaaa atatcaagcg acaggattat
      181 tttaataaag aatatacgct ggtcttggaa gatatgcacg acgaaaatgt tatttctcgg
      241 caaggtgtgc tatttttcat agaccgtatt ctacgtgatc acacaaccaa tgaaaagctt
      301 ttccagatta taaattagcg gctaatcaga tgaaatacct ggcacggcaa ttattggcaa
      361 cggcagtact gtcatctatg tcattaccta ccaacatggg gtcaaacccc agtgactaag
      421 ccgtatccgg aagcggatgc cggacaagca ttaattaacc caggtgaatc aaccgggaat
      481 attcagcacc gaactttgtg tgattaattg attaaacata aaaacaagta atgaatttcc
      541 caaatggggg acgcattcgg tctcctattg gatggcgatg ctcgtggctg taggaacttt
      601 tttattgggg gccgcggcgt atgtgtttac ggcagctatc atcattccta ttgcggattg
      661 cttaacagta cttgcaacca gcgggccggc agatttgcat ttcctggtgc acggtttgac
      721 cgctgtttat atgatcataa caagctgcct ccttttcaag aactccagcc aaatacagcc
      781 atgagcacgc taattaccct gaaggtgttg ctgttgctgc atttgacaga gttgacctta
      841 atggcattac cggcccgcgt cccctaatac cccggattct gcgtatacaa ccccggcgcc
      901 gcatccaact gcgactgcgg caccggataa ataacataat tcgccaccac agtattaatc
      961 ctatgctgca catcttcttt gctgatccag tcgttcataa tagtcaacac ctgtccgctg
     1021 cgttgcagat cgaaccacct gaagccttca tcggcaaatt cttttctgcg ctcatcgaac
     1081 aattgcgtga gtgtcacccc cgtcttgctc gccagacctg cccgggtcct caccagattg
     1141 acgatggagt ctaccgtagt ctgatttccg ggcgccccgt tcaggatgca ttcggctttc
     1201 atcatcagta tatcggtata ccggatagcc atgaagttga tcccccagtc gaaccggctc
     1261 gtcgtcggga tcttcgttac gtccaggtat tttttaaaga aagggcggag ttccgtgcta
     1321 ccggcattag tatatcctgc tgtatagatg gtggcaggca gccgcaggtc tcctgtctca
     1381 taatcggcta ccaggtcctg cgacacaggg atgatctcga gactcccgtt cgacttcgta
     1441 tccttaagcg ataaaaaata gccgttaggc gtcagatccc agggataggt ggcaccataa
     1501 gtggatgccg ttccgctgat cccggtttga tacatgacgt caaagacggc ttccttattt
     1561 ccagtggagg tcgggctctg gttggaataa gagaagatat tcgcatagtt ggggttgaaa
     1621 gcgaaaagcc cgctgccgac aatatcgttt attaaaggca gcgccagcgc ccattcgttg
     1681 agtgccagac ccggcccgtt gatactgtag gcaggtcccg atcttgccat atatacttgc
     1741 gccagcagac cttcggcggc atatttggtt gcccttccca cgtcaactcc cgtatagctg
     1801 gccggtagat tcgcaatcgc aaactgcaga tctgaaatga tcaatgcata cacatcggtc
     1861 actgaacttt gtttgaggtt gttggcttcg ctggttgtca tgggatgatc gacgatgggc
     1921 aatctgccat aatatcttac gagatcgaaa taatagaagg cccgcaggaa cttcgactcg
     1981 gcttgcaaac gcgtagccag gctcgctgat cctacattgc tgccgttctt gcttagttga
     2041 tccagggcgg tattagcccg gaagatgcca ttgaaatccg tgttccagcc ttcttctaca
     2101 tatgtattcg aggcgatgcc tgacgaaaag tcattgatag ggtcccagtc gcgcacgacg
     2161 acggacacac catagatatt gtcggaccgg atctcagaga gattcagcaa acggtcagga
     2221 taacccctca gatccgaata ggctgcgttc actccctgga taaaatcact gggggtctta
     2281 taaaaggttg ccgtggtggc ggatgaaatg ggtatctggt tcaggtcctt ggtgcaggaa
     2341 gtgcccacga ggataagaag gaggaaacca ggaaaaaagt tcttattcat tgtatgcatg
     2401 attgtaatga tcaaaaattg atgttcagtc cgacaataag cgattttgcc agcggcaggc
     2461 cgccgtagtc gcccgcttcc ggataagcgg aattggtact gatggtggta ttggcagctt
     2521 cggggtttag gccgccggag tatttgtcat ggccgaagaa attctcggct gaaacataca
     2581 aacgtgcgtt ctgaacgacc gatgtcctga tcaactgttt caaattatat ccaaacgtga
     2641 tattccttac acgtatataa tcggaagaat atagccagtc ggtattcgct acgaacccaa
     2701 acgtcgagta cgccttgctc acgcggccgg cgccaggatt ggtcggagac tcccatcgat
     2761 ggatataaaa ttcgggggca ttgtccgtaa acccctgacc cgtacgcgta agtgcccggc
     2821 ccagcagaga atagatggac ccccccgatt gtccctgtat caatacgctc aggtcaaagc
     2881 ccttgtatct gaatacattg gtcattccat acgtgtagtt ggggttggga tgtcccacaa
     2941 tctgtttgtc cgcctccgtg ataacaccat catggttcaa atcttcgtac ctgggatccc
     3001 ccacggtctc gccggtacca tacacagcca cttttttatt gatatcatcc tgtgtaagaa
     3061 taccgatcat tttgacgaca tagatgctgt tcaatgggct tcctacgcgc tgaatattgt
     3121 cggtcacgtc aaatccattg ggaattatga tctggttctg gttgccgtac agcgcaacga
     3181 tcttattgcc gttgtggctg atgttgatgc tggtggtcca ctggaacctt ccaaccatgt
     3241 tgcgtgatgt caattcgagc tcctggccga tattccgtac ggaccccgca ttggcgagtg
     3301 cgaattgcag gcctgtcact tcgggtaccg gcacattcaa caagagctgg gtgttcagtt
     3361 tattataata gtcgaaagat cccgtgagcc ggttgttgaa aagtccgaag tcgatgcctg
     3421 catcataggt ctgcgaccgc tcccatttga gattggggtt ggcgatcaca ttgggtgcct
     3481 gccctatcgc ggccccctgc ggggatccta atacgtagcc atacgatcca agcgtaggga
     3541 tgtcataatc gccgatattg ttattgccat tcacgccata gctgcctctc agtttcaggt
     3601 cgctgaccac ccctaatcta ttcataaatt tttcctggat tatcctccat cccaggctca
     3661 ccgagggaaa atttgcatac tggttgttgg cgccgaatct cgacgatgca tcccggcgca
     3721 aactggccga taaaaagtac ttctgtttat aatcgtactg caaacgactg aaataagaga
     3781 tcagcacatt ctggctcgat acgttattgc cagtgaaacc gatgtccgcg gctgcattca
     3841 gggtctgtat cgacgcgctg gtaaatcccc ctacggacgt gatgcccatc tggtccagcc
     3901 ggtctttgtt gtacgcctgc cccactaaaa agttcagact atggtcttcc gctatcacgg
     3961 cattataggt cagggtattt tcattgacca gggtcagccg acggtagttg gaatacgttc
     4021 cggaggtagc cgccgtcagg tttggattgg tagctgcgtt aaacgtcctg gccgtcaccg
     4081 taccctgggt tatatatggc acatacgtgc tggtattgct gttgacattg tccacgttca
     4141 acgttgaccg gaaggccaac cctttgtaga gttgtacctc gccatagata atgcccaaca
     4201 cgcgtgccct cttggtctgc ccgatattat aggtcaattt gcccaggctt ccgttggtgc
     4261 tgttactcca gagatactgt gcatttttcc cgatgttagg ataggccccg acgctatcct
     4321 cctgcacagg tgacatgctc agcgcctggt ggaaaatgtt gtccttccct tctacacccg
     4381 gatcgtcggt aatggaatag gaaggcgaaa cggcgacccc gagccgtagg atcttgctcg
     4441 cgttgacatc cacattcgca cgtgccgaat attccttata gtctactcct ttgatgaacc
     4501 cgtcctgtct ggcatagttg cccgcgaaat aatatcggac ggcatcggtg ccgccgctgg
     4561 ccgaaagacc cgcattctgc ataggacctt tccgctcgat gtcgctctgc cagtctttga
     4621 attccagacc cggatgacct ggcgtcgacc accggggatc cagcatatag gccacgttca
     4681 cctgaccagg cgcaagccct attttggcgg ccctcgtcgc tgggtcgtca cccgccgtag
     4741 cacccgccgg gccatatttc aatacgtagg cagcgttgat catttccgtc gcacggtcga
     4801 tccattgctg accgttcagc atttgtaatt tcttggcggc ctcgttatat cctgcatacc
     4861 ccgatacccc gatcaccggc ttcccatttt tgccgtgccg tgtagtgatg atgaccacgc
     4921 cattggaagc cctggatcca tagatagcag cggctgcggc gtccttcagc acttcaacat
     4981 tctcgatatc gttcggatta atattgtcca tcgggttgcc acccacaaaa ctcccgttac
     5041 ccgtattcga ggaattattt gatatcggca tcccgtccac cacatacaat ggctcgttcc
     5101 cggcgctgat agagcccgaa ccacggacct ggatgctgaa tgctttgccg ggaataccgg
     5161 tattttgctt gacggtgaca cccgccacct gccctacaag ggcctgatcc acccgggtta
     5221 tcggccggtc gtcaaacgca tccgatctaa gcgtggcgac ggcgccggtg acattggccc
     5281 gtttttgcgt gccatacccg atgacgacga catcgctcat tgaagtcccg gcgatcttca
     5341 tggaaagcga caattcgtgt cgcgaattca gtttgatctc ttgttgttca tatcccacat
     5401 aactgaatac aaggacgacg ttgcccgaag gaacggtcag ctgatatgac ccgtcggcac
     5461 cggatacagt ggaagtccgc gagcctttca cggaaacggt cacattcgtt aacggtttac
     5521 cggtctcgtc cgatactttt ccggaaacct gaaaattttg ttgggaaaaa gagagcatac
     5581 ataggcgcaa taggaacgtt agcaatagtc cccgccgcaa aagcgcattt gatttcataa
     5641 aagcagcaat tatggttata aataggccag tatcctaaaa gtagactaac agctcaccgc
     5701 aactgtcctg aagcatcctg cttatatgag aaaatctacg caatcgttcc cggcccgtaa
     5761 tcagggtaat aattatcaag agaaagataa aaaagtatca aagcgtaatc ctccgtcgtc
     5821 tcaaatacag gatcagaaac gccggttcac catgtcgccg gtttcgcgta tattgctaat
     5881 acagcaataa tcaccccctt cacatgagag gtctaatgat ggactatcag ctcaccgtcg
     5941 ctgccattct ccgaagggtg gagagcctgt ttgcacacaa acccatcatc acccgtctgc
     6001 ctgaccgatc catccaccgc tatacgtatc gcgattttgc aaaacgcgtg aagcgattgt
     6061 ccgtggcact gcagcggctt ggggtaggcg agggcgacag agtggcgacg ctttgctgga
     6121 accactacca gcacctggag gtctattatg ccgccccctg catgggtgcg gttatgcatt
     6181 ccctcaatct taggctcaac ccggcagacc tgtcatatat cgtcaaccat gccgaagaca
     6241 aggtcatcat cgtcgaccag gtgctgctgc cactgttcga gcaattcaga gacaagatca
     6301 aagttgcgag catcattgtc atccggcagt gcgaagagcc gctccccgct ggatatcttg
     6361 actacgagga cgttatcgcc aagggtgatg ccgcgcaatt ccggcccttc gaaggcgatg
     6421 aaaattccgc cgccttcctg tcctacacat cggggacaac cgggaagccc aaaggggtcc
     6481 tgtattcgca ccgttcgatc gtgctgcatg ctatgggcag cctgatcagt tgcgccgggt
     6541 tcggcatcac cgagcgggat gtggttcttc cgatcgtccc tatgtttcat gccagcgcct
     6601 gggggcttcc attcaattgc gccctcgccg gcgcggcgct cgtccttccg ggtccttatc
     6661 tcgatgcgga aagcctgctc cagctgttcg aaaacgaaaa agtgacgatc acggcggggg
     6721 tgcccacggt gatgcttagc atcttaagca gactggaagc ggagccgcaa aaatacaagc
     6781 tctttctgcg taccatcgtg atcggtggct cggcggcccc gcgatcgatg gtcaagacct
     6841 tcgacgagcg ttacggcata cagctgcttc actcctgggg tatgacggag atatctccgc
     6901 tgggaagcac cgcgactccg accagccgac tgtcggcggc gtcaaaagaa gtgcagtacg
     6961 actattcgat gaaacaaggt ttgcctgtcc cgctggtaga aattcgtgga cgcaacgagt
     7021 cgggactcat tccatggaat ggagtgacga tgggcgagct ggaggtccgg ggaccctggg
     7081 tggcgtcggc ctattatcgg gatgaagggc cgtcggcgaa gttcacgtcc gatggctggc
     7141 ttaagaccgg cgacatcgtt accattacag aagagggatg tatcgagatc aaagaccgaa
     7201 gcaaggatgt tatcaagtca ggtggtgaat ggatcagctc catagcgctg gagaacgcac
     7261 tgatggggca tccttccatt ttggaggccg ccgtgatcgc cattcccgat agcaggtgga
     7321 tcgagcggcc cttcgcctac gtggtgccga aggaaggaaa gacggtgtcg gtggaggagc
     7381 tatgcacgtt cctggttaac aaattcccga aattctggct tcccgacggc tacgccgtcg
     7441 tgacggcgat accgaagacg tcggtcggta aatttttgaa gtcggcgctt cgcgaagcct
     7501 acgcccgcga gcacccggga gacggcgtct gatcatcgat cgccatgccg cgccgaccct
     7561 agtcatcgat cgcctggtat accagcggct tgaactgttt ctgcgcatcg gcgacatgtg
     7621 gcccccacat ctgcatatac agcaacacga tcatatcctc cttcgggtcc acccaataaa
     7681 cggttgaaaa cactcctccc catccatagc ttcccgcctg gctgggcatc aaccggctcc
     7741 ctttttccga ctcgatcgac acgccgagtc cccacttgta ctcctcacct cctatattga
     7801 taaaaagatc gccgatctgg ttcgtcgtca tcatccgaac agtattgcgt gacagcagcc
     7861 gcacgccgtt gtactccccg ccgttgagca acatctgcaa aaaaatggca tagtcggaga
     7921 tcgtcgatga cagtcctcct ccacccgaaa aataatccgt gtgttgcagc gggaagttca
     7981 tatcgagcgc gccgccgaac gtcttgtcct gccggtgtat gcccgaagag tcctccatga
     8041 aaaaattgac cagccgcgaa cctttcgacg ccggcacgtt gaaataggtg tcgttcatcc
     8101 ccagcggccg aaagatcctt ttcgaaaaga attcctccag cgacatcccc gaccagatct
     8161 ggacaagata tcccagcacg tcatgcccga gtccatacat ccatttctct cccggctgga
     8221 acatcagtgg caacttgccc agccggttca tcgcatcggc aagggtttgt tgttttacac
     8281 caaccccgcc ggtcatcgcg tcttttgcat agatcgcgtt ggcttccgga gtgcctatcg
     8341 ccgggtaacc aagtccgctc gtatgcgtga tcagatcgcg aatggtgatg ggacgtttgg
     8401 cggggacggt ggtatatgtg gtgtctttcc aattgaaact agccaatacc ccctggtggg
     8461 cgaaggacgg gatgtacatc gacaccgggt catcgagtcc gaatttccct tcttcccaca
     8521 gcatcatgac ggccaccgag gtgatggcct tggtctgcga ggcgatacgg aagatatcgt
     8581 ccttgggcag aggcgctttc ttaaccaggt cattgtaccc atacgctttg taataaacga
     8641 tcttccccct gcgggctatg aatgccaccg atccgttgat ccatttcttt tttacccagt
     8701 cgctcatgcc ggaatccagc cgggcaagcc gggcagcgga gaatccgccc gacccggggg
     8761 cgccgggagt cagcaccgac cgatctttct cttgagggaa tacgttagcg atggacgcca
     8821 gcagtataac caggatggtt agacaccgtt tcatagccgt taatttttgg ggagataatt
     8881 tataatagga aattaatgga tatatttagt acctccaaat aatcagatga gactgcaaaa
     8941 caagatcgcc gtgatcaccg ggggagcggc tggcatcgga ctcgccaccg ccgaacggtt
     9001 tgccaaagaa ggtgcaaagc tcgttttatg ggacgtttcg gataaaggaa atgaggtggc
     9061 gcagcggctc cggaaggaag gccacgacgc catcttcaaa aagatcaacg ttaccgacca
     9121 ggacgaggtg cagaccgccg tcggcgaagc ccgcgaccat tttggtcgta tcgatgtcct
     9181 catcaacaat gcgggtatca cgagggacag gacgctgctg aagatgtcga agaacgagtg
     9241 ggacgatgtg ataggggtga atctttcagg cgttttctat tgcacacagg cggttgcgcc
     9301 cattatgaaa gagcagaact atggccgtat tgtttccgcg tcgtcgaatg tggccattcg
     9361 cgggaatttc ggacaaacca actacgttgc gaccaaatcg gccatcattg gcatgacgaa
     9421 ggtgtgggcc atggagctcg gtcgctatgg gatcaccgtc aattgcattg ccccgggatt
     9481 cattcatacg gctatgaccg acgccatgcc cgaagaggtg cgaaaacaaa gcatccccca
     9541 tatcccgctg ggtaagtggg gttatccgga agatatcgcc aacgggtatc tctatctggc
     9601 ttccgatgag gcttcgttcg tgagcggcat ttgtttgacg atcgacgggg gggcggcgcg
     9661 gccatgaaaa gaatattgct tgcgatttgc ctcaccacgg cggcatggcc ggctcttgcc
     9721 caaaaagagg tcatctatca tattttccaa cgcagcttct tcgacgccaa tggcgacggc
     9781 atcggcgatc tccggggtct gaaggaaaag ctcggttatt tgcagcagct gggcgtcacc
     9841 tccatactcc ttacgcctct gtataaatcc gtgttctatc ataattattt cgcggacgac
     9901 tggtatgcca tcgaccccgg ttacggatcg ataaaagact tcaccggtct cgccagggca
     9961 ctgcatgttc gcggaatgaa attgtatatc gacatggaga cgcaatatgt caccgatcgg
    10021 cacccctggt ggacctccgc cgtcggcaat ctccgatcgc cctgtcgcga ctatctgctt
    10081 ttcgaggact ctgctcatca gaaacccgct ccgatcatct ggggcatcac cgagctgaaa
    10141 ggatacaatg gcgacagccg caaggtcacg accgtcaacc ttcacgaccc cggcgtgctc
    10201 gcatataacg aaaggctttt ttcctttttc gcagacccca atggcgatgg gaaattcgac
    10261 gacggcgtag acgggttccg gctggatcac acgatggatg acctcgacgg caacccgcgg
    10321 cttggggacc tgttcacccg attttgggcg ccattgatcg accggctcaa aaagctgaac
    10381 ccgcggctga cattcgtcgc ggagcaggcc aactgggccg actttggttt cgactatttc
    10441 aggagcggaa agctcgatcg ggtgtttgct ttccggcttg ctatggcaat tcagtccttc
    10501 gataagagcc aactcgaaaa ggccgcggat accacgctcc ggcaactgcc gccgggtaag
    10561 gaacaggtgg tctttatcga aaaccacgac ttggaccgat ttgccagccg catcgataac
    10621 gacgcaggca agctgcgggt tgcggcggta ttgaatatcc tgctcggggg gatcccctct
    10681 atttattatg gccaggaact cggcatgttc gggaaaggag ggaaatatgg aatgaccgac
    10741 gcgaatgaca tcccccgccg cgaagctttc gaatggtatg ccgccgacac cgggagaggc
    10801 atggcgcttt ggtataaggg taccggtcca tggtgggaca gcacgaacct tataccacac
    10861 gatggggtct cgctggaaga agaacggagc gattccgggt cgctttgggt ttggtaccgg
    10921 cgactactgg aagttcgaaa agaattcccg gtcctgcaaa ccggtgtata cgattcgctc
    10981 cataacggga atccaaaagt atttacctgg cgccggcggg acgcacgatt caccgcttat
    11041 cttgctgtca atctttccga tcagccgcaa cagacgacga tcgaaacaga gcgggtagtg
    11101 cgcgacgtct ttggaaatgc caccgtgccc tccggtcaaa aacgcttcac gatcaacctc
    11161 tcaccgtacg gagcaggtat ctggatgacg gtcgacgccg aggcatcccg gaaataactt
    11221 tagttatgtt gcttttcctc gttgatccgg ctaagtttgc gcaaacgcta accctttttc
    11281 gtgaaaccga tatatcttct tctgcccata gccttactgc tgcgcgctgc cagcgcccag
    11341 caacccttcg ccagcgccca acagcaacaa cccgccgccg ccaggcaacc cgccgccgcg
    11401 agccttgccg attatggcta taacataccc gctggctgga ccccgaccca atatcccgac
    11461 ggtatcgtcc tcagcgcccc cgtctcgaat acagctgaga gatgtctttt gcaaatttgg
    11521 cctatgcgca caccttccgg caatctggcg caggatgcgg tcacggcgtt cagacaggtg
    11581 ttcagcgcct atcaaccgac ggagagtcaa tatgccacac gcaattccgt catccgtggg
    11641 acctcggcgc agggatggga ttatttcatg atcaaaaatg ccatccggct ttccgagggg
    11701 aattaccagg tcatgtgggg gtttgtcttt gtcgccggac tcggcaaccg ggtggccgtg
    11761 atctcgggca tctcgaagga ccccctggtc agctcctgtt tcgggctcaa cctgagtgat
    11821 gtatggccga aattctttta cagcctccgg ttcaaaggct ggaattcttc cttatcaccg
    11881 caacagatga tgaagcggat agcgggatcc tggatagccg taacggcgac ggccggggac
    11941 aaatgggtct tcgccgccaa tggccgtttt gccagcgcct ccgcggcaca acgctactat
    12001 tctatttcct ccaatgaagc tatcagcgtt accgatgcgt atttcggcga cgggtcatgg
    12061 tcgctcgacg ggcaggccat cgtcctcacc aaagacaatg ataaggcccg gccatcgaag
    12121 ggctggatca ggctggagca ggaaagctat gacaatggta acaactgggc cgagcacctc
    12181 tatttgctac gaaccagcac ggtggatggg aaagaatatg aaatgaacta tcatcgggat
    12241 aaatagcggc tttgctattt cttcaacagt ttatcatcga tgatcttcat cactgcctcc
    12301 ttataatccc gcttccttca ccagcagttc catcccttcc tgggccagtg gttcatcatc
    12361 cactatgtat gatgagccgc agtggttgta tgaacagatc gatcgctgga tacggacagc
    12421 cgggtcggtc agttcgagac aaaggtagtg acgctcttcg gcaaaagggg atagctgaac
    12481 gaccccgatc cgacattcac cggattgtca ctggaaagac ttttatctgc cgatgtgacg
    12541 tagggggtaa acgaagacgg cgacgtcccg gtgaccgaaa aaggctggtt gaccacggtt
    12601 gtacccatat tgaccaccac gatgacgagg cctgttccat gtttataagc ggtgacatac
    12661 acgttgttgg acggactata agtcacgctg atccgctgag acccgggccg cacgaatttg
    12721 gcaaactgcg ccatgacata accgcgtttt gtcgggttgt cattctgatc gatcggtccg
    12781 taactgcgtt tcaaatacca ccagttgtac acgctgaaac cggccgtgcc catgcaatcg
    12841 ctgatctctt ttgccgtatt cagcgcgccg gtccaatcgg tactggtatc gagatgctcg
    12901 gtcatccaga cctcttttcc cttcgatacc gccagcgggt agggagcgag ccctgcgcca
    12961 tagatatgac cgccgacgat agacagatgc gaggccgctg tcgaatcgtt caggataggg
    13021 tccgtatagg aaggatcgaa atgaaaggat tcgctggcga tcagccttgt gtttttgatc
    13081 gcgggcgcgt tgtatttcat aaaattgaac agctgttggg ccgtccagtt gcaggattca
    13141 taagtcacgc tgatatcggg ttcattctgt atcgaaagcg catacaacgg cgctccgttg
    13201 gcggacatat atacggcaaa actatccagg taattggcgt aggcggcata agatgcggta
    13261 tccagcactc ccccgatagt gctgccgttg gatttgaaat tggccggtgg cgaccatggt
    13321 gtcgccatca cgatcgcgcc gtttgcaatc gctttttgag cattcccgag ctcgacaccc
    13381 caattgcccg gaccgccggg atcgacgcgg atgcgcaata ccgtgagacc cagctgtcct
    13441 gattgagttc cataaaggag attcatctcg gccgtggtca gcgcgccgag gaatacggtc
    13501 gctcctccga agccccggat gatctgggct ccattggaag gatcgatggc cgcagatgtc
    13561 ccctgtatgg tatcgggttt tgatggcccg cccggattga ccggtggcgg cgccggcgaa
    13621 ttcgatttgc tgcaagcgat cgcaaagagc agggcaagcg ggataagacg aagttctcgg
    13681 gagctcatat agatggtgcg gtagctatct ttggtccacg ctccgcacca taaaaataag
    13741 agaaaatatc aagattgcaa tcgattgcaa tcgcgaactt ggaccagccc ctatgtggat
    13801 aactatgtgc ttaacgggtg cagaactgca gcagaaaaaa tttggaatcg ggggtttggt
    13861 agttgtatct taggtttaca attgagagac ggaaaatcga taaaaagaaa ggaagtctct
    13921 taagggaagt ggaagttctg aaagttacca cccgaaaggg tgaagacaaa gaagaatcgg
    13981 aaacaaccca gaaaagcatc gggtttagta agtagaggaa accttggtcg aaaggctttg
    14041 gtcgagacta ctgaaaacga cttggtgctt tttgttttta tataccttcc cgccggccac
    14101 acacccggga gatcactctt ccacctgcca tccctcctct ccaccttcga acttcaacgg
    14161 cagccatacc gtaaacaccg ccccctcgcc tttttgggac tccgccataa tatatcccct
    14221 gtgattcttc acgatcttgg ccacaatact aagtcccacg cccgtaccgg aaaactccga
    14281 cttgggatgg agccgctgaa aaagcttgaa gatcttatcg gtatactgct gctcgaaccc
    14341 gatgccatta tcggcaaacc gtatctcggc gtaggtttgt ttcggcgggc catccggtag
    14401 caccccgatc ttgtcccccg tgacgatctc cgagctgatc cggatcacgg gcttcttacc
    14461 cgcgaatttt attccattgc ttatcaggtt gctgaacagc tgctgcatct ggaaaggaat
    14521 ggcctccacc gtcggcagat gatcgctgct gatctccgcg ccgctctccc ggatgatcag
    14581 ctcgtaatcg gtttttacat tcttcaatat cgtattcaga tccgtggacg cggcggaagc
    14641 cgtcacctgc gaaagacggg aatattccag cacactctgg ataagctttg acatgcgttg
    14701 cgatgactcg ttgatctttc cggcatattt tttccaggcg gcggggtcat cgcgacgctc
    14761 ctgcagcagc aggatgaagg tctggatctt tcgcagcggc tcctgcagat cgtggctggc
    14821 gatataattg aaggattcca gctctttgtt catctgcttc agctggaacg ttctacgcgc
    14881 gacctccgct tccagcctgt cttccacttc cgcccttttc cgcgcgatgc tgacttgtgt
    14941 cctaaccctc gacagcagct cattccccgc aaacggcttg acgagatagt cgtccgcccc
    15001 cgtctcatac ccctccatcc tcgattcctc tcccgcgcgc gcggtgagta acacgaccgg
    15061 aagccgggcg gtccttttgt cctgtctcaa cgtcttcagc aattggatgc catccatctc
    15121 cggcatcatg atatcggaga gcaccagggt cggcatcacc gtgtctatct ttttcagcgc
    15181 ctcctttcca ttggaggccg tcactacatg ataatatttt tgaaggatag aagcgatatg
    15241 attgctcata tcggaattgt cgtctaccac aaggacggta tctttcaggt caccgccatc
    15301 ctggtccggc tcctgatatg gcatgtgttc gaacagcgcg ctggcttccc cgatatatac
    15361 cccggaatgc tcttcctcat aggggatacc cgctacctgt cccgacggca aatgatcttt
    15421 accggtggga agtgtgacgg tgaccgtagt accttcgccc tcgacactct tgatgccgat
    15481 gtcgccacaa tgcaggtgga ccagctcttt gatcagagag aggccaatgc ccgttccttc
    15541 gatggtccgt ccccccgaat tctccacccg gtgaaaacgc tcgaacatat gcggtagctc
    15601 tttttccggg atacctgtgc cggtatcgct gacttccagc aatacctggt gttcgcggaa
    15661 aaagagccgc acggagatgc ttcccttcag cgtatattta aaagcattgg acagcaggtt
    15721 gaacacgatc ttttcccaca tgtcccgatc cacgtaaaca ggttcaccgg gctcgtcgtc
    15781 cgtccccacg acaaactcca gaccggcctt ttcgatcacc gaccggaaat tgctggccag
    15841 gttcttcgtc agcccggcga tatcggtcag ggtgtaagct gccttttgtc tgccgctctc
    15901 gatacgactg aaatccagca gggtattcac cagccggagt aaccggatcg aatttcggtg
    15961 ggcgatctcg agccggtcat gatcgctcac ggaaagggcg gattctttca gcagctgttc
    16021 gatggtcccc aggatcagcg tcagcggggt ccggaattca tggctgatgt tggaaaaaaa
    16081 gatggttttt gatttgtcca gctcctccag ctgctgggac ctccggcgct cttcctcata
    16141 ggcgaatgcg ttgttcaagc cgagtgagac ctggtcggcg atcagctgaa aaaagttatg
    16201 gtagctgtca tcgaattgcc ggtaggggtt gagccccgcc acgataacgg ccaccggtgc
    16261 ccgactgccc gactgcctca ccggcacgca cacatagaaa gagggggcta cgtcccatgc
    16321 gcctttcgcc cagccgcccg tttccggtgc catcgggaaa acgacgatct tcctttgctg
    16381 ggccgcgacg aggatattct cctgcatggc ttccggcgcg tcttccactt cgtcctttct
    16441 tatccggtag atcgacgcga aagggatatc ctggggattg tttgccagga cttccagcgt
    16501 ttttgaatag acttcatctt ccgataacgt ggcagctatg tttttcccga gatcacggag
    16561 cgtttccatc tggcgccggc cgacgaccgt tgccgtgatc tcattgaccg tagccaggac
    16621 gcccccgata cccagggagt cctcattgtc cggaacaggg ctgtaggcga tggtgaaatg
    16681 agtttcctcg gtgaaattat tccggttgag tatcagtgtg atgtcctcca tccaggtggc
    16741 ggggccgcca ttgaatgggg aatcgatcag gggcttcaga atatgccata tctccgacca
    16801 gccttcggat gctggtacac cgaaggacct ttgcggatgt ttcagacccg ttatagggat
    16861 atagggatca ttgtacaact gtatatagtc ctctccccac cataagatca tgggaaatcg
    16921 attggcaagc atcagaccga cgacattctt gaggctgggc gaccattccg acagaggtcc
    16981 aagcgtcgtc ttgctccagt caaactgacg gatccggtgt gtcatttccg tgttgcccca
    17041 cagaaaatcc aattgttttt ctttaacact ccgcatatcc gttattcctt tttagggttt
    17101 cgcatcacct cctccagttg tcgctgcaag ccggaaatgc ttatctcttt cttgatgaac
    17161 cccgcagctc ccagccggat acagcttttg acgatagccg gctcggccga tgtcgaatac
    17221 atgtacaccg ggacagactc gagactgtgg atcttcttga tctccatgag gcattccata
    17281 ccgtccatac ggggcatatt gatgtcgaca aatatcttat cgggaacgaa ggcgcgatcg
    17341 gctttaagta tttcgagcgc atcgataccg gtatctgcgc aaatgcattt gatcgtagga
    17401 aaagcattct ctacggccat tacgaaaaat tcctgatcgt cggtatcatc ttcgatcaaa
    17461 aaaacgaggg gttccttcgc catgcatttt ttatcgtaaa ctgttctaac agcataaata
    17521 acgccggatt tagcccacgg attagctaag tggataccca tagagtttat ccccggggac
    17581 caccggcggc cacgaattga gtaatttatt ccccaatctg ggatatcttt aaaggcactt
    17641 acactaccga tccgcacccg tgcaccccat aggcttaccc ttaggctcac acgtaccggc
    17701 atcgacaatt gcaatcaaac agccaaaata tggccatcca acatgcgatc atttccccta
    17761 tccgcggctt ataccgattt gtacgaactg accatgggag aggtgttttt ccgtgagggg
    17821 aaacaggata ccgcagtcgt cttcgattat tttttccgga aggtgcccgg cggcggtgga
    17881 tatgtgctgt ttgccgggct ggaggatttg cttgagaccc tttcagcatt gcggtttacg
    17941 gatgaggaca tcgcctttct ccgggatcag ggtttccatg cctcctatat cgattttttg
    18001 cgtgggtggg aatttaaggg gatgatcctt tccgcgcgcg aaggggagat cgtctttccc
    18061 ggctgtccta tccttcgtgt cgaaggagga ctattcgaag cacagctgat cgagacctta
    18121 ttgctgaata tcctgaattt tgaatccctg atcgccacca aggcttcccg gataaaatac
    18181 gtagcgggtg accgtgtggt cagtgatttt ggtcttcgtc gttcgcacgg ccccggcggt
    18241 atcctggcca ctcgcgcggc gttcgtggga ggagcgacaa gcacgagcaa tgtctatgcg
    18301 gcggagaaat atcatctgcc cgtcaccggt acgatggcgc attcttttgt cgaa
//
LOCUS       DASYSV010000141         4873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1306817, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000141 DASYSV010000000
VERSION     DASYSV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306817"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(363..1397)
                     /locus_tag="VGQ51_04440"
     CDS             complement(363..1397)
                     /locus_tag="VGQ51_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEV9035839.1"
                     /translation="MGSTTTTSPTLTSKDFATDQEVRWCPGCGDYSILAQVQKVMPTL
                     GIPRENIVIISGIGCSSRFPYYMNTYGMHSIHGRAAAIASGLKATRPELSVWVVTGDG
                     DSLSIGGNHTIHLLRRNFDINILLFNNQIYGLTKGQYSPTSEEKKITKSTPFGSIDHP
                     FNPLALARGADASFVARSMDRDPKHLQQMLLRSHAHKGASFLEIYQNCNIFNDGAFEI
                     FTEKGSKPLETLFLEQGKPLVFGANRDRGIRLDGFHPVVVQLGGEFSESDCWIHDEHD
                     FFKAQILVRMFDDPRIEGHLPRPFGVFYETERPCYEELLNLQIEEVVKSKGKGDLDKL
                     LRGSETWTIS"
     gene            1540..1830
                     /locus_tag="VGQ51_04445"
     CDS             1540..1830
                     /locus_tag="VGQ51_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035840.1"
                     /translation="MKKTSLIMAIIIGCSMAMTGCAVDGYVTDEPADVVYSRPVTPGE
                     GYVWIDGDWVWASGTYHWHNGYWSRPREGRTWAPGHWEHSGRGYHWNRGHWR"
     gene            2046..2456
                     /locus_tag="VGQ51_04450"
     CDS             2046..2456
                     /locus_tag="VGQ51_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV9035841.1"
                     /translation="MATDLFTTIPLHQFVDELTAGLLPRAVSRKSIIINQIDRDLQVG
                     SDENLLAFVLWNLLDRAVDSTQNECIHIESVCLGERTMIRVRNAGVYFYRSCANCFRQ
                     MQDAAEKLGGSISIDSMDMETTVALTLHKDLTAA"
     gene            complement(2558..2887)
                     /locus_tag="VGQ51_04455"
     CDS             complement(2558..2887)
                     /locus_tag="VGQ51_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV9035842.1"
                     /translation="MNIYVSNLSFNVTDEDLQDYFAEYGEVSSAKVITDKFTGKSRGF
                     AFVEMPDDAAAQKAIQELDGASVDGRTIGVSVAKPREDRGNGGGGNRGGGGGFQRRNN
                     NFSNSRY"
     gene            complement(3099..4307)
                     /locus_tag="VGQ51_04460"
     CDS             complement(3099..4307)
                     /locus_tag="VGQ51_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9035843.1"
                     /translation="MQQTEKEYLKRSQIVTMAICTGLIVANIYYCQPLLVLISQTFRI
                     PESEAGLIAFFTQVGYAVGLLFFVPLGDKVERRRQIVWMAGFAVASLVFAALSPNLLC
                     LEIASVFIGASSVIPQLILPMAAHLAAPSRTGKVIGAVMSGLLIGILLSRTLSGFIGD
                     WLGWRGMFAVAAGISFLLLLIIRFSFPVSRPGFSGSYGSLMRTLLTLVKQQPVLREAA
                     AINALAFATFGLFWTTMVLHLHAAPFHFSTHVIGLFGLAAAAGALAAPLVGGSADKRN
                     PRIPIGYGLVLLGLSFVLMYARAGSVVGMIAGIVLLDLAMQGIHVSNQSRVYALLPAA
                     RNRLNTVYMTVSFTGTSLGSAAGLYAWKLAGWNGVCLTGVLLTAAAATIYVLTYKRER
                     PSAGNPLVSG"
     gene            4455..4850
                     /locus_tag="VGQ51_04465"
     CDS             4455..4850
                     /locus_tag="VGQ51_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV9035844.1"
                     /translation="MLHKRKIAVLVALLAVLFVGMAAMRPQDDKPKRNLKVLPKDISK
                     DSLDHVMHGWAHALGVHCNFCHAPAGGDSTSRRLDFASDAKPEKTAARHMYQMVGKIN
                     KKFFKNDQGVAVISCITCHRGSPHPDEVK"
BASE COUNT         1211 a         1232 c         1276 g         1154 t
ORIGIN      
        1 cgaccagtcc cctgtcctga caagtttttt ttcgttaccc tatctgaagc gatcgtttcc
       61 ggtaagcaca gtctgtctgt ctacccccat gatcagttac ccggatctta ttgtcgtaac
      121 caggactagt gcaaattcac atatatgcca actttttctt tttgtggttt cagaggtaag
      181 cacaggccgg cgatttctat cgctggctct tttatttgat ctgaaacggg cgcatagatt
      241 ccgaatagaa tcgatgcgta gcattggaaa agaaaaaaac ttcactgtga aaaaaccaaa
      301 aaagaggcgg ctcaatcctg tcgggccgac ctctttttat tttatgtacg ccaggattga
      361 aatcaggaaa tcgtccaggt ctcgctaccc cggagcagct tatccagatc acctttcccc
      421 ttgcttttta ccacctcttc gatctggaga ttgagcaact cctcataaca gggacgttcc
      481 gtctcataaa agacgccgaa ggggcgtggc agatgacctt cgatccgcgg atcgtcgaac
      541 atgcgtacca ggatctgtgc tttgaagaaa tcgtgttcgt cgtgtatcca gcaatcgctc
      601 tcgctgaact cgccgcccag ctgcactacg acaggatgga atccgtcgag acggatacct
      661 ctgtcccggt tggccccaaa taccagcggt ttaccctgtt cgaggaacag ggtctccagc
      721 ggcttgctac ctttttcggt gaagatctcg aacgccccat cgttgaagat gttgcagttc
      781 tgatagatct cgaggaagct ggcgccctta tgtgcatggc tgcggagcaa catctgctgg
      841 agatgtttcg gatcgcggtc catgctccgt gctacgaagc tggcgtcggc gcccctggcg
      901 agggccagcg ggttaaaggg atggtcgatg cttccgaatg gcgtcgattt ggttattttc
      961 ttttcctcgg aagtgggcga atattgtccc ttggtgagcc cgtatatctg gttgttgaaa
     1021 aggagaatat tgatatcgaa gttgcggcga agcaggtgaa tggtatggtt gccgccgatg
     1081 ctgaggctgt ctccgtcacc ggtgaccacc catacgctga gttcggggcg cgtggctttc
     1141 aggcctgagg cgatggccgc ggcgcgacca tgtatcgagt gcatgccata cgtgttcatg
     1201 taatagggaa agcggctgct gcagccgatg ccagaaatga tgacgatatt ctcccggggt
     1261 atccccaggg tcggcatgac cttctgtacc tgcgccagga tggaatagtc cccgcatccg
     1321 ggacaccagc gcacttcctg atcggtggca aaatctttcg atgtcagggt cggtgatgta
     1381 gtagttgttg agcccatgcc tacaaattta atgaaataat cgtcgttttt ggggaattga
     1441 caccacaaat accggctctt gaccctccgg gacggtatgg catactaatt ggccttttat
     1501 cctgtagagt cccttaatac tcatttaaaa atcttgatta tgaaaaagac atcattgatc
     1561 atggcaataa ttataggatg cagtatggcg atgaccggct gcgccgtgga tggatatgtc
     1621 accgatgaac ctgcagacgt ggtctattca cgacccgtga ctccgggtga aggatatgtc
     1681 tggatcgatg gagactgggt ctgggccagc ggcacctatc actggcataa tggatattgg
     1741 tcgagaccga gagagggccg tacctgggct cccggtcatt gggaacattc cggaagaggg
     1801 tatcactgga accgcggaca ttggcgctag ttccaaggtt ctgtctgcat atatcgaagc
     1861 ctgtagccaa cgctacgggc ttcacttttt tagatacggg ttcatttttt agatgggcct
     1921 aaggtccatt tgctaaatag attatttatt ttccgaaaat aaaaccttag agcgctgact
     1981 aaagtatgtc tcattgcgtt acatttacac ggttaaaagc aaccctctct aaaatcctgt
     2041 atcaaatggc tactgatttg tttaccacta tacccctgca ccagttcgtg gatgagctga
     2101 ctgccggact cctccctcgg gcagtaagtc gtaagtctat tataatcaat caaatagatc
     2161 gtgatcttca agtaggttcg gacgagaatc tccttgcctt cgtattgtgg aatcttctgg
     2221 accgggctgt ggacagcact cagaatgaat gtattcatat cgaatccgta tgtctggggg
     2281 agagaacgat gatccgtgtc cggaatgccg gagtgtattt ttatcgcagc tgcgccaatt
     2341 gtttccggca gatgcaggac gccgccgaga aactgggcgg gtcgatcagc atcgactcga
     2401 tggatatgga gacgacggtt gcgcttacac tgcataaaga cctcacggcc gcctgacgct
     2461 caatcttaca gccggatcat caggccgaat aaaatacacg cattacaaaa taaaagggct
     2521 cccatatggg agccctttcc tatcgacaac gaatagctta gtatctgctg ttgctgaagt
     2581 tgttgttcct gcgctggaaa ccgccgccgc cgcctctgtt accgccgccg ccattgcccc
     2641 ggtcttccct gggtttggct acggatacac cgatggtcct tccatcgacc gaagcaccgt
     2701 ccaattcctg gatggctttt tgagctgctg catcatcggg catttcgaca aacgcaaaac
     2761 cgcgggattt gccggtaaat ttgtcggtaa tgaccttggc ggaagaaact tctccgtact
     2821 ccgcaaaata atcctgtaag tcttcgtcgg tgacgttgaa acttaagttc gaaacgtaaa
     2881 tgttcataca aatgactggt taaaaaaaat aattaaaaac aatctgagta aaaaagcagg
     2941 gagtaaatac gactaataat taccgggaga tttgacttag cggaaatatt cattcaatta
     3001 ctaatgctgt tatcagctca aatttgacta atgcaaatgt acaacaaatt cgattatctc
     3061 caagcaaatc ccgcatgaaa atctcaatta gtaatttgtt aacccgaaac taagggattc
     3121 cccgcagaag gccgttccct tttataggtt aatacgtata tggtcgcggc cgcagcagtc
     3181 agcagaacgc cggtcaggca aacgccgttc caaccggcga gtttccacgc ataaagtccg
     3241 gcggcggagc ccagagaggt cccggtgaag ctaacggtca tgtatacagt attcaaccgg
     3301 ttacgggccg ccggaaggag ggcatatacc cggctctggt tggagacatg gatgccctgc
     3361 atagcgaggt ccaggaggac gatcccggct atcataccaa cgaccgaacc ggcccgggca
     3421 tacatcagca cgaaggacag cccaaggagg acgaggccat aaccgatcgg gatacgggga
     3481 ttgcgtttat ccgccgatcc gcctaccagc ggcgcagcga gagcgcctgc cgccgcagcc
     3541 agtccgaaga gcccgatgac atgggtgctg aaatggaacg gcgcggcatg gagatgcagg
     3601 accatggtcg tccagaacag gccgaaggtg gcgaaggcca gggcattgat agcggctgcc
     3661 tcgcgcaata ccggctgttg tttcaccagc gtgagcaggg tccgcatcag cgagccgtat
     3721 gatccggaaa agccgggacg gctgaccgga aaactgaatc ggatgatgag cagaagcagg
     3781 aaactgatgc cggccgctac cgcgaacatc ccgcgccagc caagccagtc gccgatgaat
     3841 ccgctcagcg ttcgtgacag gaggatcccg atgagcagtc cgctcatgac ggcgccgatc
     3901 acttttcccg tccgcgaagg agccgcgaga tgtgcggcca tgggcaggat cagctgtgga
     3961 atgacggacg aggcgccgat gaatacgctg gcgatctcga gacaaagcag attgggtgac
     4021 agcgcggcaa acacgagcga ggccacggcg aaacccgcca tccagacgat ctggcgccgg
     4081 cgttcgacct tatcgcccag tggaacgaag aacagtaacc cgacggcata tcctacctgc
     4141 gtaaaaaagg cgatgagccc cgcctcactt tccggaatgc ggaaggtctg gctgataagg
     4201 acgagcaatg gctgacaata gtagatattg gctacgatca atcccgtaca aatggccatg
     4261 gtcacgatct ggcttctctt caggtactcc ttttccgtct gttgcataag cccgcaaaga
     4321 tagcaaggaa catcgacccg tcttctaaca gaagccacta agttattttt tggggcgtct
     4381 ccgaaaatga atttgtcaga atattgtcat tttatcggca gcattcccta atttagagaa
     4441 ccaaaaattt ctctatgctt cataaaagaa agattgccgt gctcgtcgcc cttctcgctg
     4501 tcctgttcgt cggcatggcc gccatgcgtc cccaggacga caagcccaaa agaaacctga
     4561 aagtgttgcc taaggacatc agcaaggata gtctcgacca tgtcatgcat ggctgggcgc
     4621 atgcactggg tgtgcattgt aatttctgtc atgcgcctgc cggcggtgac agcacctcac
     4681 gccgtttgga tttcgccagc gatgccaagc cggagaaaac agcggctcgt cacatgtacc
     4741 agatggtcgg caagatcaat aagaaattct ttaagaacga tcagggtgta gccgtcatta
     4801 gctgtatcac ctgtcatcgc ggaagccctc atccggatga ggtaaaataa ttattaaata
     4861 atttgcgata tat
//
LOCUS       DASYSV010000142         7172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_410317, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000142 DASYSV010000000
VERSION     DASYSV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410317"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VGQ51_04470"
     CDS             complement(<1..309)
                     /locus_tag="VGQ51_04470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEV9035845.1"
                     /translation="MNSPHPLVSCLCLTRQRPLQLKRAIGSYISQTYRPLELLVLVRD
                     DDPESKAVVLSYGRDDIRLIEFAGQPERSLGELRNLVISHSRGEYFCQWDDDDWYHNE
                     R"
     gene            complement(322..2739)
                     /locus_tag="VGQ51_04475"
     CDS             complement(322..2739)
                     /locus_tag="VGQ51_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035846.1"
                     /translation="MFKNSLKLAWRNLLKDRLFTLLNLVGLATGLACTLLIYLWVNDE
                     LHMDRFNSDDSRLYQVMKNSPTPTGIETVPNTPGLLASTLARQMPDVEYAVAVIPPSW
                     FDRKGIITYQGHLFEASGQFAGKDFFNVFSYDLLRGNREEVMKDKGAVLISDDLARRV
                     FGSADDVVGKSIEWNQKDFSGVYPIAGVFRKPSANSTSQFDVLFNYDLFVDKYPKMTD
                     WGNSDPSSYVVLKPGTDAVAFDRKIAGLIKANSHDSQETLFMQKFSDKYLHNHYENGA
                     PSGGRIEYVRLFSIIAAFILVIACVNFMNLSTAKAVSRMKETGIKKVIGAPRYTLILQ
                     YLSESLLMSFLSALVAVGMVLLVLPQFNTITGKDMHPDFGAGFILTVLAITLMTGIIA
                     GSYPAIYLSSFQPAAVLKGKLKSKVGEMMVRKALVVFQFTLSGIFIIAVLVIYRQMQL
                     VQTINLGYNRDHLVYFDKGGMAFADKQDYAPGGRYEASLDNFLQRVRSIPGVATAANF
                     RHNITDRNGGTYDLSWPGKDPNSRIDFTDLGVGYGFIETMGIQMLEGRPFSRDFGSDR
                     SSIIFNETAIKKMGIKDPVGKVIHVWGEDRQIIGVVKDFHFQSLYENIKPCFFDFTFN
                     QRAAKIMVRLKAGQEKLALDGLERLYREENPGIPFEYKFLDDDYQALYASEKRVAALS
                     RYFAGLAVIISSLGLFGLSAFMAQKRQKEIGIRKVIGASTGSVVLLLSKDFVQLLLIA
                     ILVAFPVSGWVMDHWLKGFAYRVPMKADIFLIAGLLICFITLLSVSFQSVRAAISSPI
                     RSIRTDG"
     gene            complement(2836..3768)
                     /locus_tag="VGQ51_04480"
     CDS             complement(2836..3768)
                     /locus_tag="VGQ51_04480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV9035847.1"
                     /translation="MARDIIAVTWGGLGDVLMCTPAFHAIKEAHPDRRVIVYCSREDH
                     RNVLLQNPNIDSLRVLLPKYMWRYPRHLYAYLFNSEGLKYYDSKRFKFYYLFFQHVPS
                     VLYEQKHVMDILPEIFGDLAIDVSRKKCELYFTRNEEEKARSAVAPYKNIVFVHVHSK
                     CSENHHWLPERWNELVASLPQYTFIQVGGKNETPVRSAVDWRGRTGLREAFCLFKHGD
                     AFVGVDSCFAHVANAFNVPGVVLFGDSSPVFVGHNTNINIYKGLSCSPCEEYLNGHAC
                     PIGHSCMNLITVEEVRESLLRQLAVRKRAYVEKV"
     gene            complement(3761..3964)
                     /locus_tag="VGQ51_04485"
     CDS             complement(3761..3964)
                     /locus_tag="VGQ51_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035848.1"
                     /translation="MSALREPKEILERIGQVEAHLRLIEENFRQEMTKPYFHRRRNFC
                     IFLHLEKKWYGEILKELKWVLNG"
     gene            complement(3981..6035)
                     /locus_tag="VGQ51_04490"
     CDS             complement(3981..6035)
                     /locus_tag="VGQ51_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEV9035849.1"
                     /translation="MNCSFARIGAVCLLILFSSFGFRPQGKGKILWDRFGVPHIYGSD
                     LSQMYYEFGWAQMHNHANLVLRLYGQARGQAAAYWGDQYLASDKQVRLFQIPELAEKE
                     YSRQQPAFRKWLDAFANGINAYATAHPDAISTENRQVLPVRGQDVLAHGIRIIFLRFV
                     AGDNLGLPSRTGKAGSNGYAIGPSRTLSHHTLLLANPHLPWGDLYTFFEAHLQAPGFN
                     AYGAAVVGLPVLNIAFNLHLGWTLTVNTIDACDRYELSLQGDGYILDDHTVPFEKKEV
                     SLRIKQADGSLREERMECLYSRHGPVIGSQEGKAYAIRIAGMENPDILYQWHRMAAAR
                     DSKEFETALKMMQLPMFNVIYADDKGHILYLFDGNVPDRSEGDWKFWNGTVDGRFSKY
                     IWDRTLPYEDLPRLWDPVTGFVQNANDPPWSCTYPALLDPGKYRLYISPSGMPLRPQR
                     AVNMVRRDSFVTAERLAGYKLNTGMEAADRFLNDLLAAVQAYPDSLAAAAAAVLQKWD
                     RMTDSDSRGAVLFARWFDKMTPEMFHTPWSPGDPLTTPAGLKDPRAAVHLLDLAAEEI
                     LKEDGRLDIAWGEVYRFRLNEMDYPANGGSAQYGIYRTIYYARDKDGKYRAVAGDSYV
                     AITEFGKEVKAWVLLSYGNASQPGSKHAGDQLLLLSRKQLRTAWLGRKDILLNLEEKE
                     IF"
     gene            complement(6046..>7172)
                     /locus_tag="VGQ51_04495"
     CDS             complement(6046..>7172)
                     /locus_tag="VGQ51_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV9035850.1"
                     /translation="QKQQEQLIHALDFPTENRLQLVETLLDELPEVVRGQFKVCFCGP
                     TGSDAVEAAIKLAKHKTGREGVIAFHGSYHGMTSGALSATSNVSLRSRLGSLVPHIDF
                     IPYSYCYRCPFGKDRDSCDLECAGYLRYILENPHSGIVRPAAILLEPIQGEGGSIVPR
                     AGFLEEVVRIAREKDIVVIFDEIQCGFFRTGDCWGFQGSRAIPDIITMSKGLGGIGFP
                     ISAILYNTSIESWGAGDHIGTFRGNQVSIAAGNGAFDFVRRYDIATHVKEMGEYLMSR
                     LRRLKEEFSVIGEVRGEGLIIGIEYVKDPLSRTPDPEIARKIRKNCFQAGLLFELGGH
                     YGNVIRFLPPLIVNRQIIDNALAIFERAHEAVMATASAVN"
BASE COUNT         1657 a         2049 c         1810 g         1656 t
ORIGIN      
        1 ccgttcatta tggtaccaat cgtcgtcgtc ccattggcag aaatactcgc cgcgcgaatg
       61 gctgatcacc aggtttctca gctctccaag cgatcgttcc ggctgtccgg caaattcgat
      121 gagccggata tcgtcgcggc catacgacag tacgacggct tttgactccg gatcatcatc
      181 acggacgagg accagcagtt cgagggggcg atacgtttgg gaaatatacg acccgatcgc
      241 tcttttcagc tgcaatggac gctgtctcgt cagacagaga caggatacga ggggatgtgg
      301 ggaattcatg gatctttgcg gtcagccatc ggtccggatg ctcctgatcg gactggatat
      361 ggcggctctc accgattgga agcttacgga gagcaaagtg ataaaacaga tgagcaaccc
      421 agctatgagg aagatatccg ccttcatggg cacccgatag gcaaatcctt tcaaccaatg
      481 gtccatgacc caccccgaca cggggaatgc caccaatatg gctatcagca gcaactgtac
      541 aaaatccttc gatagcagca ggactacgct gccggtagat gcaccgatca ctttccttat
      601 accgatctcc ttttgcctct tctgcgccat aaaagccgat agcccaaaca gccccagaga
      661 ggaaataatg accgccaacc cggcaaaata ccgggagagg gccgcgaccc gtttttccga
      721 agcatacaat gcctggtaat cgtcgtcgag gaacttgtac tcgaaaggta ttcctggatt
      781 ttcttctcta tacagccgct cgagaccatc gagcgccagc ttttcctgac cggcttttaa
      841 ccgcaccatg atcttggcag cccgctggtt gaaggtaaaa tcgaaaaaac agggtttaat
      901 attttcgtat aaggactgaa agtgaaagtc tttcactacg ccgatgatct gccggtcttc
      961 gccccataca tggatgacct ttcccaccgg gtcttttata cccatctttt tgatggcggt
     1021 ctcattgaag atgatactcg atctgtccga accgaaatcc cgggaaaagg gcctcccctc
     1081 gagcatttga atgcccatcg tttctataaa gccataccct acccccaaat cggtaaaatc
     1141 gatccgtgaa ttggggtctt tcccgggcca cgacaggtca taggtacccc cgttacgatc
     1201 cgtgatatta tgtctgaaat tggctgccgt agccaccccg gggatgctcc ttaccctttg
     1261 cagaaagtta tccaggctgg cctcgtatct tccgccggga gcataatcct gcttatcagc
     1321 gaaggccatc ccgcctttgt cgaaataaac aagatggtcg cggttgtacc ccaggttgat
     1381 cgtttgtacc aattgcatct ggcgatagat gaccagcacg gcgatgataa agatgcccga
     1441 cagggtgaac tggaacacga ccaaagcctt tctcaccatc atctcgccta ctttgctctt
     1501 tagtttgccc ttaagcacgg ccgcgggctg aaaactcgaa agataaattg ccggatagct
     1561 gcctgcaatg atgccggtca tcaatgtgat cgccagcact gtaaggataa aaccagcgcc
     1621 gaaatccggg tgcatgtctt ttcccgttat cgtattaaat tggggcagca ccagcaggac
     1681 catcccgacc gccaccagtg ccgagagaaa tgacatgagc aaagactcgc tcaggtattg
     1741 caggataagt gtgtaccgcg gcgcacctat cacctttttg atacccgtct ccttcatcct
     1801 gctcaccgct ttggccgtgg acaggttcat gaagttgacg cacgcgatca ccaggatgaa
     1861 tgcggcaata atggaaaaca gccgcacata ttctatcctg ccccccgaag gcgctccgtt
     1921 ctcgtaatgg ttatggagat atttgtctga aaatttctgc atgaacaagg tctcctgcga
     1981 gtcgtgggaa ttggctttga taagacccgc gatcttcctg tcaaatgcca cggcatccgt
     2041 accgggcttg agcaccacat aggagctggg atcactgttc ccccaatccg tcatctttgg
     2101 gtacttatcc acaaacaggt catagttgaa gagaacatcg aattgggagg tggaattggc
     2161 agaaggtttc ctgaagaccc cggctatggg atagaccccg ctaaagtcct tttggttcca
     2221 ttctatgctt ttgccgacga catcgtcggc ggagccaaag actctcctgg ccaggtcgtc
     2281 ggagatcagg acggcgccct tatccttcat tacctcctcc ctgttccccc gaaggagatc
     2341 ataggagaaa acgttgaaaa aatctttgcc ggcgaactgc ccgcttgctt cgaaaagatg
     2401 accctggtac gtaatgatcc ctttcctgtc gaaccacgaa ggagggatca ctgccaccgc
     2461 atattcaacg tccggcattt gccgggccag ggtcgacgcc agcaggccgg gcgtattcgg
     2521 cacggtctct atacccgttg gcgtcggcga atttttcatc acctggtaaa gccggctgtc
     2581 gtcgctgttg aacctgtcca tatgcagctc atcattcacc cataggtaga tcagcagtgt
     2641 acaggccagg ccagtagcga gaccaacgag attgaggagg gtgaaaagac gatccttcag
     2701 gagattgcgc caagccagtt tgaggctgtt cttaaacata cgataagatt gaatggtgtt
     2761 gacaatgtgc ctgccagaaa gacggattgt caaacgaaat gttgcatccc gaagttatcg
     2821 atttttccgt aaatattaaa ctttttcgac ataagcgcgt ttacgcactg cgagctgtct
     2881 gagaagggat tcccggacct cttccaccgt aatgaggttc atgcaggaat ggccgatcgg
     2941 gcaggcatgt ccgttgaggt attcttcgca gggcgagcag ctcaggccct tgtagatatt
     3001 gatattggta ttatgcccga cgaagacggg cgaggaatcg ccgaacagga caacgccggg
     3061 cacattgaag gcattggcga catgcgcaaa acaagaatcc actccgacga atgcgtcgcc
     3121 atgtttgaac aggcagaacg cttcccgcaa tcctgtcctc cccctccagt cgacggcgct
     3181 cctcaccggc gtctcattct tcccgcccac ctggataaag gtatattgcg gcagggacgc
     3241 taccaattcg ttccatcttt ccggcaacca gtgatggttc tccgaacatt tggaatggac
     3301 gtgcacaaag acaatgttct tatacggcgc aaccgcggat cgggcctttt cctcctcatt
     3361 ccgggtgaaa taaagctcgc atttctttct actcacatcg atggccagat ctccgaatat
     3421 ctcgggcagt atgtccataa catgtttttg ttcatacagg acggatggca catgctgaaa
     3481 gaacagataa tagaacttga aacgcttgct gtcgtaatat tttagcccct cgctgttgaa
     3541 gaggtaagca tacaaatgac gcggatatcg ccacatgtat ttcggtaaaa gcacccgtag
     3601 cgaatcgatg ttcggatttt gcagcaatac gttccggtgg tcctcgcgcg agcaataaac
     3661 gatcaccctg cgatcggggt gggcctcttt gatcgcatgg aaagcgggcg tacacatcag
     3721 cacgtctccg agaccgcccc aggttacggc aatgatatct ctagccattt aggacccatt
     3781 ttagctcttt caggatctcg ccataccatt tcttttccag gtgaaggaag atgcagaagt
     3841 tccgtctccg gtggaaataa ggtttggtca tctcctgacg aaaattttcc tcgatgagcc
     3901 ggagatgtgc ttcgacctgg ccgatgcgtt cgagtatctc cttcggttcc cttaaagcag
     3961 acatagtaaa attagatttg tcagaagatt tctttctcct ccaggttcaa caagatatcc
     4021 tttctgccca accaggcggt gcggagctgt ttacgggaaa gcaacagcaa ctggtcgccc
     4081 gcatgtttgc tgcccggttg cgaagcgttc ccatagctga gcaggaccca tgctttcacc
     4141 tctttgccga attcagtgat ggcgacatag ctatcccccg caacggcccg gtatttccca
     4201 tccttatccc gcgcatagta gatggtccgg tagatgccgt attgcgccga cccgccattg
     4261 gccgggtaat ccatctcgtt tagccggaac cgatagacct ctccccacgc aatatccagg
     4321 cgcccgtctt cttttagtat ttcctcagcc gccagatcca gcagatggac ggctgcccgt
     4381 ggatctttca gcccggccgg agtcgtcagc gggtccccgg gactccaggg cgtatggaac
     4441 atctccgggg tcatcttgtc gaaccaccgg gcgaacagca ccgctccgcg gctgtcgctg
     4501 tccgtcattc tgtcccattt ttgcaacacg gctgcggccg ccgcagccaa tgagtcggga
     4561 taggcctgca ccgccgccaa cagatcgttc aaaaaccggt cggcagcctc catgcctgta
     4621 ttcagcttat aacccgccag cctctctgct gtgacgaagg aatctctcct caccatattc
     4681 accgcacgct gcggccgtag cggcatgccc gacggggata tgtatagccg gtatttcccg
     4741 ggatccagca aggcaggata tgtgcagctc cacggcggat cattggcgtt ctgcacaaaa
     4801 cccgtaaccg ggtcccatag cctggggagg tcctcataag gcaacgtccg atcccagata
     4861 tatttcgaaa accgaccgtc cacggtaccg ttccagaatt tccaatcccc ttcagaacgg
     4921 tccggtacat tgccgtcgaa caggtacagg atatgccctt tatcgtcggc atagatgacg
     4981 ttgaacatgg gcagctgcat catcttcaat gccgtctcga actccttcga atcgcgggct
     5041 gcggccatcc ggtgccattg atataaaata tcgggattct ccatgcccgc tatccggatg
     5101 gcatacgcct tgccttcctg cgacccgatc accggtccat gcctggagta aagacattcc
     5161 atcctttctt cccggagact gccgtcggct tgttttatcc gaagggacac ctcttttttt
     5221 tcaaatggta ccgtatgatc atccaggata tacccatccc cttgcaggct cagctcatac
     5281 cggtcgcatg catcgatggt attgacggtc agggtccatc caagatgtag attgaaggct
     5341 atgttgagga cgggcagccc caccacggct gccccatacg cgttgaaccc gggagcctgc
     5401 agatgcgctt caaaaaaggt gtacaggtca ccccagggaa gatgcgggtt ggccagcaac
     5461 aaggtatgat gtgacaaggt cctggacgga ccgatggcat agccattgga gccggctttg
     5521 ccggtccgcg aaggcaaccc gagattgtcg cctgccacaa aacgcaaaaa gatgatccgg
     5581 atgccgtgcg ccagcacatc ctgtccacgg accggaagca cctgcctgtt ctccgtactg
     5641 atggcatccg gatgcgccgt ggcataggcg ttgatcccgt ttgcaaacgc atccagccat
     5701 ttccggaatg caggctgttg ccgggaatac tctttttctg ccagttcggg tatctggaaa
     5761 agccgcacct gcttatccga ggccagatac tgatcgcccc aataggcggc agcctggccc
     5821 ctggcttgtc catacaaacg caggaccagg ttggcatggt tgtgcatctg tgcccaaccg
     5881 aattcataat acatctggct caggtcgctg ccgtaaatat gagggacccc aaaacggtcc
     5941 caaaggatct ttcccttacc ctgtggccgg aagccgaagg acgagaagag gataaggagg
     6001 catacagccc ctatcctggc gaaggaacag ttcatatact attgattaat tgacagccga
     6061 tgccgtcgcc atcactgctt cgtgcgcgcg ttcgaatatc gcgagggcat tgtcgatgat
     6121 ctgcctgttg acgatcaatg gcgggagaaa acggatcacg ttgccatagt gaccacccaa
     6181 ttcgaacagc agccccgcct ggaaacaatt cttccggatc ttcctggcga tctccgggtc
     6241 cggtgtccgc gataaagggt cctttacgta ttcgataccg atgatgaggc cctcgcccct
     6301 cacttcgccg atgacggaga attcctcctt cagtcttctc agccgcgaca tgaggtattc
     6361 gcccatctcc tttacgtgcg tcgcaatatc atagcgccgt acgaagtcga aagcgccgtt
     6421 gccggcggct atgcttacct ggttgcctct gaaggtcccg atatgatccc ccgcccccca
     6481 ggactcgatc gatgtattat acaggatggc gctgatcgga aacccgatac cccccagccc
     6541 cttggacatg gtaatgatat ccggtattgc acgactgccc tggaaccccc agcaatcgcc
     6601 ggtccggaag aacccgcatt gtatctcatc gaagatgact acgatatcct tctcccgtgc
     6661 gatgcgcacc acctcttcca ggaaccccgc gcgcggcacg atcgagccgc cttctccctg
     6721 tatcggctcc aggagaatgg ccgcgggccg gacaatgccg gaatgtgggt tctccaggat
     6781 atatctcagg tagccggcgc attccaggtc acaggagtcc ctgtccttcc cgaaaggaca
     6841 ccggtaacaa tagctgtagg gaatgaaatc gatgtgcggc accagggaac ccagtctcga
     6901 acggagactg acattgctgg tggcggagag ggcgccggag gtcattccgt gataggaccc
     6961 gtgaaaagcg attacccctt cccttcccgt cttatgcttg gccagcttga tagccgcctc
     7021 cactgcatcc gaccctgtcg gtccgcaaaa acacactttg aactggcccc tgacgacctc
     7081 cggcagctcg tcgagcagcg tttccaccaa ttgcagcctg ttctcggtag gaaaatccag
     7141 ggcatgtatc agctgttcct gttgcttttg ca
//
LOCUS       DASYSV010000143         5068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_29010, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000143 DASYSV010000000
VERSION     DASYSV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29010"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4012)
                     /locus_tag="VGQ51_04500"
     CDS             complement(<1..4012)
                     /locus_tag="VGQ51_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEV9035851.1"
                     /translation="MKRLVIILFLGVAYRASAGDTLDLAGTWRFRMDPHDVGETQRWF
                     AQPLKETVHLPGSMMQNGKGDPVNVNTQWTASIYDSSWFFDPRMAKYRRADNLKLPFW
                     LTPPKHYLGAAWYQKDIVVPVSRAGRHFVLYLERPHSETTVWLDDKPMGREYGFCTAQ
                     EFDLTGLLTAGHHVLTLRIDNRIKDINVGPDSHSLTDQTQGNWNGVVGRLLLTARSDT
                     WIDDVQVFPDVAGKRAHVHLLIRSLHPGGSGTIRLKAESFNTSEPKQSDSLTVSYTLS
                     EGRDSVVADLPMGEHPLLWDEFDPALYRLTVATAHDRRTVEFGMREFGIEGTHFTING
                     RLIHLRGMVENCEFPLTGYAAMDEASWERVFRIARSFGLNHMRFHSYCPPEAAFQAAD
                     RVGFYLQPEGPSWANHGSSLGDGKPIDRFIFDETNRMAYYYGNYASWCMLSYGNEPRG
                     GHQVEYLTRFIDYWKAKDRRRVYTGADVGMSWPLVPANDYMVKSGPRGLNWTSLPESE
                     TDYSPAIQKFTVPYVTHEMGQWCAYPDFTEISHYTGVYKEKNFELFQELLRGQGMGDE
                     ARRFLMASGKLQVLCYKAEIEKSLRTRGSAGFQLLCLTDYSGQGTALVGVLNALWQEK
                     GYVTAKEFRRWCGPTVPLVRMPRFVYTSKDTLSASVELYHYGAKPLRRAVIDWTLRNE
                     AHVPIAHGSFAPMDFPIGNDPIGTINIPLHATGHFKLEVSVANTAYANDWDCWVYPAV
                     LPTVANDVYYCTSLNDTARRILQQGGKVFLNAAGKIVKGKEIIQHFTPVFWNTSWFKM
                     RPPHTLGILLDPKHPAFSDFPTSYHSDYQWWDVLYQAQVMHLEDFPPGFRPLVQPIDT
                     WFLNRRLGLIFEAGVGRGRLMVSSADLGPDLPADRPASRQLYHSILHYMNTGRFQPAS
                     AVSLKVVNDLFMTPSRFVWNSYTKSSTDDLRPHPQAGAKIDRRALVGRHNVINEKVDP
                     LSSLSVGNGKFAFTVDITGLQSFPEAYDKGVPLGTQSEWGWHSWPDSAGYRFGSTLRS
                     GYSIQLKNPAVDWFRANPHRLQLGNIGFELRKQDGSLAGPGDLQDIRQELVLWTGEIR
                     SHFTLEGIPVDVSTVAHPDFDEVAFRVNSPLIRTGRLKVRIRLPYPTGAFADGGDDWD
                     HADRHRSFLVNSGDKGAEISHQLDSTTYDLQMDWSGGTIAEHQPHYFLVTPAVADSFS
                     MGCAFTQDHEARPASWTATRKASADAWNTYWTTGAAVDFSGSTDPRAAELERRIVLSQ
                     YLMRVQEAGDYPPQETGLTYNSWYGKFHLEMHWWHEAQFALWGHPELLERSLDWYFRA
                     APGAAAIASRQGYKGVR"
     gene            complement(4009..4773)
                     /locus_tag="VGQ51_04505"
     CDS             complement(4009..4773)
                     /locus_tag="VGQ51_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEV9035852.1"
                     /translation="MYKYLPILLLAGLVWRDQRPTLYIIGDSTVKNGRGKGDGGLWGW
                     GNFLDASFDTTRIAVRNYALGGRSSRTFITEGHWEQVRSQLKKGDFVIMQFGHNDGGP
                     LDDTARARGTIKGIGDETKDIYNPIMKKNETVHSYGWYMRQYVNDTKAMGATAIICSP
                     IPRDMFKDRKVLRSSADYGGWAKEVADATGAWFIDLNGIIADEYDRLGADSVKGFFPG
                     DHTHTNEAGARLNAAAVVTGLRGLNGLALDGYLKDR"
     gene            complement(4778..>5068)
                     /locus_tag="VGQ51_04510"
     CDS             complement(4778..>5068)
                     /locus_tag="VGQ51_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035853.1"
                     /translation="AHWKTMLHKVEPPEKGIPHFRDIYVSDVHVASARKAISATGLEQ
                     SLIEGVHIDRTTIHAATAGDIAFAKDWNLDQVDITAKDGSKLQIKNSSHVTF"
BASE COUNT         1086 a         1561 c         1310 g         1111 t
ORIGIN      
        1 agcggacacc cttatatcct tgtctggaag cgatggcggc tgccccgggc gcggcacgaa
       61 aataccagtc gagcgatcgc tccaataatt ccggatggcc ccagagtgcg aactgcgcct
      121 catgccacca atgcatctcg agatggaatt tgccgtacca gctgttatag gtgagaccgg
      181 tttcctgtgg cggataatcg ccggcctcct ggacacgcat caggtattgg gagagtacga
      241 tacgccgctc caactctgcg gccctcggat cggtgctgcc cgaaaaatcc accgccgccc
      301 ctgtcgtcca gtaggtattc catgcgtccg ccgatgcttt cctggttgct gtccaggaag
      361 ccggacgagc ctcgtggtcc tgcgtaaacg cacaacccat gctaaaacta tccgccaccg
      421 caggcgtcac taaaaaatag tgcggctgat gctctgcgat agtcccgccg ctccaatcca
      481 tttgaaggtc atatgtcgtc gaatccagtt ggtggcttat ctccgcgcct ttgtcgccgc
      541 tgttcaccag aaagctccgg tggcggtccg catgatccca atcgtcgcct ccatccgcaa
      601 atgctcccgt cggataaggc aaccgtatcc tcaccttcaa ccgtcccgtc ctgatcagcg
      661 gactattgac ccggaaagcc acttcatcga agtccggatg cgccaccgtc gaaacatcca
      721 ccggaattcc ttccagcgta aaatgacttc gtatctcgcc cgtccacaat accagctcct
      781 gccggatgtc ctgcagatcc cccggccccg ccaggctgcc gtcctgcttc cgcaattcga
      841 aaccgatatt gcccaattgc aacctgtgcg gatttgcgcg aaaccaatcc accgccggat
      901 ttttcaactg tatcgaatat cccgagcgaa gcgtggaccc gaaacggtaa cccgccgaat
      961 ccggccaact atgccatccc cattccgatt gggtacccaa cgggacgccc ttgtcatagg
     1021 cttcaggaaa actctgcaac ccggtaatgt cgaccgtaaa cgcaaatttc ccgttgccca
     1081 ccgataacga gctcagcggg tcgacctttt cattgataac attatgccgc ccgaccagcg
     1141 cccgccgatc gattttcgcg ccagcctgcg gatgcggccg cagatcatcc gtactactct
     1201 tcgtatagga gttccacaca aacctgctcg gagtcataaa aagatcattc accactttga
     1261 ggctcaccgc cgacgcaggc tgaaaccggc cggtattcat atagtgaaga atgctatgat
     1321 acaattgtcg cgacgccggg cgatccgccg gcagatcggg tccgagatcc gcactgctca
     1381 ccatcagtct tcctctccca acccccgctt cgaagatcag ccccagccgc cggtttaaaa
     1441 accacgtatc gatcggctgc accagcggtc tgaatcccgg ggggaaatcc tcgagatgca
     1501 tcacctgcgc ctggtagagg acatcccacc actgatagtc gctgtgatag ctggtcggaa
     1561 aatccgagaa ggccggatgt ttcggatcaa gcagaatgcc taacgtgtgg ggcggccgca
     1621 tcttgaacca ggaagtattc cagaataccg gcgtaaaatg ctggatgatc tcttttcctt
     1681 tgacgatctt gccggcggcg ttcagaaaaa ccttgccgcc ctgctgcagg atacgccgcg
     1741 cggtgtcgtt cagcgaggtg cagtaataaa cgtcgttggc cacggtcggc agcaccgccg
     1801 gatacaccca gcaatcccag tcattcgcat aagccgtatt ggcgacggag acttcgagtt
     1861 tgaaatgacc ggtggcatgg aggggaatat tgatcgttcc gatcgggtcg ttaccgatgg
     1921 ggaagtccat gggtgcgaac gagccgtgtg cgatcgggac gtgcgcttca ttacggaggg
     1981 tccaatcgat gacggcccgc cgcaatggtt tggcgccata atgataaagc tcgacggatg
     2041 ccgacaacgt gtccttactc gtatagacga accggggcat ccggaccagc ggcaccgtcg
     2101 ggccgcacca tcgccggaat tcctttgccg tcacataccc tttctcctgc cacagcgcat
     2161 tgagcacgcc gacgagcgcc gtaccctgac cggaataatc ggtgagacac aggagctgaa
     2221 acccggcaga ccccctcgtc cgcaacgatt tctcgatctc ggccttataa caaagaacct
     2281 gcagcttgcc ggaggccatc aggaaccggc gcgcttcatc gcccatgccc tgcccgcgca
     2341 gcagttcctg gaaaagctcg aagttctttt ccttatatac gcccgtataa tgcgatatct
     2401 cggtaaagtc cggatacgca caccattgac ccatttcatg cgtcacatat ggaacggtga
     2461 atttttgtat cgccggagaa tagtcggtct cagactcggg tagagacgtc cagttgagtc
     2521 cacgcggccc cgatttgacc atataatcgt tggcagggac cagcggccag ctcataccta
     2581 cgtccgcccc cgtatatacc cggcgtctgt ctttcgcctt ccagtagtcg ataaagcgcg
     2641 tcagatactc cacctggtga ccgccgcgtg gttcgttgcc gtatgacagc atacaccagc
     2701 tggcatagtt gccgtaataa tatgccatcc ggttcgtctc atcgaagatg aatcgatcga
     2761 tcggttttcc gtccccaagc gagctgccgt gattggccca cgaaggtcct tccggctgaa
     2821 gatagaaccc gaccctgtcc gccgcctgga acgccgcttc cggggggcaa tacgaatgga
     2881 accgcatgtg gttgagaccg aagctgcggg caatgcggaa tacgcgctcc caggacgctt
     2941 cgtccatcgc ggcataaccc gtcaggggga attcgcaatt ctccaccatc ccgcgtaaat
     3001 ggatgagtcg tccgttgata gtgaaatgtg tcccttcgat gccgaattcg cgcataccga
     3061 attcgacggt gcgcctgtca tgcgcggttg cgacggtcag ccggtacagt gcgggatcga
     3121 actcgtccca taacagcgga tgttcgccca tcggcagatc ggctacgaca gaatcgcggc
     3181 cttcagaaag agtatagctt accgttagag aatcgctttg tttgggttcg gatgtgttga
     3241 agctctccgc cttcagccga atagttcccg aaccgccagg atgcagactg cggatgagga
     3301 gatgaacatg cgcgcgttta cccgccacat ccgggaacac ctgcacatcg tctatccagg
     3361 tatcggaacg ggctgtcagc aggagtcggc ctacgacccc gttccagttg ccctgggtct
     3421 ggtcggtgag gctgtgcgag tccggtccga cattgatgtc ttttatacga ttgtcgatac
     3481 gaagcgtcag gacatgatgc cctgccgtta gcagccccgt cagatcgaat tcctgcgccg
     3541 tacagaaacc atactcgcgc cccatgggct tgtcatccag ccagacggtg gtctcgctat
     3601 gcggtctttc gagatacagc acgaagtgcc tccctgcccg ggagacaggt acgactatgt
     3661 ctttttgata ccaggccgcg ccgagatagt gtttgggcgg tgttagccaa aaaggtaact
     3721 tgagattgtc cgcccggcga tacttcgcca tccgggggtc gaaaaaccag ctgctgtcat
     3781 agatgctggc ggtccattgg gtattcacgt tgacagggtc gcccttgccg ttctgcatca
     3841 tcgagccggg taagtgcacg gtctccttca gcggctgcgc aaaccagcgt tgtgtctctc
     3901 caacatcatg cgggtccatc ctgaaacgcc atgtcccggc cagatccagc gtatccccgg
     3961 cgctggcgcg atacgccacg cccaaaaaca atatgatgac caatcgtttc atctgtcttt
     4021 cagatatccg tcgagcgcca gcccattcag cccgcgcaat cccgtaacca ccgccgccgc
     4081 attcagccgt gccccggcct cgttggtatg cgtatgatcg cccgggaaga accccttcac
     4141 gctatccgcc cccagccggt catactcatc ggcgatgata ccgttgagat cgataaacca
     4201 tgcccctgtc gcatcggcaa cttctttcgc ccaaccgcca taatcagccg atgaacgcaa
     4261 caccttcctg tccttgaaca tgtccctggg tatggggctg cagataatag ccgtggcgcc
     4321 catagcctta gtatcgttga cgtactgccg catataccag ccatagctat gcaccgtttc
     4381 atttttcttc atgatagggt tatagatatc ctttgtctca tcgccgatcc cttttatcgt
     4441 gccgcgcgca cgggctgtat cgtccaacgg ccctccgtca ttatgcccga actgcatgat
     4501 cacgaaatcg cccttcttca actgcgaacg aacttgctcc caatgcccct ctgtgataaa
     4561 ggtccggctg ctacgtcctc cgagcgcata gttcctgacg gcgatacgcg tagtatcgaa
     4621 cgacgcatcg agaaaatttc cccagcccca gagtcctccg tcccctttgc ctctgccatt
     4681 tttgacggtt gaatcaccga tgatatacag cgtcggccgc tggtcgcgcc agaccaggcc
     4741 ggctaacagg agaatgggaa gatatttata catgggatta aaaagttacg tgggaagaat
     4801 tctttatctg caatttcgat ccgtctttgg cggtaatgtc cacctggtcc aggttccaat
     4861 cctttgcaaa ggcgatatcc cccgccgtcg cggcatggat ggtcgtgcgg tcgatatgca
     4921 cgccctcgat cagcgattgc tccagcccgg tagcggatat ggccttccgc gcgctggcta
     4981 catggacatc ggaaacgtag atgtccctga aatgcggaat gcctttttca ggcggctcca
     5041 ctttgtgcaa catcgtcttc caatgcgc
//
LOCUS       DASYSV010000144         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2459274, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000144 DASYSV010000000
VERSION     DASYSV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2459274"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1831)
                     /gene="glgX"
                     /locus_tag="VGQ51_04515"
     CDS             complement(<1..1831)
                     /gene="glgX"
                     /locus_tag="VGQ51_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEV9035854.1"
                     /translation="MTIYPGNPYPLGSYWDGKGVNFAVYSHNATGVELCLFDTEKGVG
                     ETQKIGLNERTHDVWHGYLPDLKPGQLYGFRVGGPYDPSNGHRFNPAKILLDPYARAF
                     CNIGDWDDTLFGYTIGQDDLVRDDRDDAAVAPKSIVIDHRFDWAGDRPPKVAYFNTVI
                     YETHVRGFTKCFPGIPDDIKGTYAALAHPTVIAYLKKLGITSVELMPVHQSMSERHLV
                     EKGLNNYWGYNTIGFFAPAHRFYSGRELGGQVKEFKNMVKMLHKAGIEVILDVVYNHT
                     AEGNQLGPTLAYKGIDNSEYYRLADDKRFYFDYTGTGNTLNARLPNVLQLIMDSLRYW
                     VTEMHVDGFRFDLASALARELHDVNRLSAFFSLIYQDPVISQVKLIAEPWDVGEGGYQ
                     VGQFPPGWAEWNGKYRDCMRDYWRGEGSMLGEFAERFTGSSDLYKNDNRNPTASINLI
                     TAHDGFTLNDLVSYNDRHNEANKEDNNDGHSDNRSWNCGAEGPTEDIGINILRARQRR
                     NFLATLFLSQGVPMLLAGDEMGHTQQGNNNAYCQDNNISWIDWEKADQDLIKFASLVI
                     KMRRDHHAFSRKMWFKGREVRKVGLTDIAWFKPDGTEMTEGDWN"
     gene            complement(1861..2214)
                     /locus_tag="VGQ51_04520"
     CDS             complement(1861..2214)
                     /locus_tag="VGQ51_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035855.1"
                     /translation="MMNAPDSMATTSSQVIEKLRQNHAGDEFRWTPGGFTLGKGKSYS
                     PQELEILKTYRFEGASDPSDMEIIYVIRANDGQLGYSLDAYGAYSSHDNEAGYDNFIR
                     QIPERGHDQQLTFEL"
     gene            complement(2295..2990)
                     /locus_tag="VGQ51_04525"
     CDS             complement(2295..2990)
                     /locus_tag="VGQ51_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035856.1"
                     /translation="MAPTTAKKTPRTSSRKKTKAKKDLSASFNKFKEFNGKLYTGVQI
                     GHGHHWNYDAGDWKETKITPDLWEISYAVTKRRVGHAPDFSGVPVGTEYHWYILAHQT
                     AVKRNANDYDTKMTGLKFKLAHKRADKGKWSASGPTQRKHLIAFLKDFIRRLEKDPIP
                     IEFEYQGKRFKGEGVPVPQTCAKGFCYELDITLNNETLGIIRYGASGWKMDLLHDQPL
                     IDAIGACIIEWFE"
BASE COUNT          617 a          800 c          835 g          770 t
ORIGIN      
        1 cgttccagtc gccttcggtc atctcggtgc cgtcaggttt gaaccaggcg atgtccgtga
       61 gaccgacctt acggacctcc cttcctttga accacatttt acgcgagaac gcatggtgat
      121 ccctccgcat tttgatcaca agcgacgcga atttgatcag gtcctgatcc gccttttccc
      181 agtcgatcca tgagatattg ttatcctggc aataggcatt gttgttgccc tgctgggtat
      241 gtcccatctc gtcgccggcc aggagcatgg gtacgccctg tgaaaggaac agggtggcga
      301 ggaaattacg tcgctggcgg gcgcgcagga tatttatgcc gatatcttct gtcggcccct
      361 ctgctccaca gttccaggac cggttgtccg aatggccgtc attgttgtcc tctttgttgg
      421 cttcgttgtg gcggtcatta taggagacga ggtcgttgag cgtaaagccg tcgtgggccg
      481 tgataagatt aatgctcgcg gtaggattcc ggttgtcgtt cttatagaga tcggagctgc
      541 cggtgaatcg ctccgcgaac tcgccaagca tcgaaccctc gccacgccaa taatcgcgca
      601 tgcagtcacg gtatttaccg ttccattccg cccacccggg gggaaactgc ccgacctggt
      661 atccgccttc gcccacgtcc cacggttcgg cgatcagttt cacctgcgag atgaccgggt
      721 cctgataaat aaggctgaag aaagcgctga gccggttgac gtcatgcagc tcgcgggcga
      781 gcgccgaggc gaggtcgaaa cgaaagccat cgacgtgcat ttcggtcacc caataccgga
      841 ggctgtccat gatcagctgg agcacgtttg gaagccgggc gttgagcgta ttgccggtgc
      901 ccgtgtaatc gaaataaaaa cgcttgtcgt ccgccagccg gtaatattcg ctattatcga
      961 tacccttgta ggccagtgtc ggtcccagct ggttgccttc cgccgtatgg ttgtatacca
     1021 catcgaggat gacctcgatg ccggccttgt gaagcatctt gaccatgttc ttgaattctt
     1081 tcacctgccc gcccagctcc ctgccgctgt aaaaacggtg tgcgggtgca aagaacccga
     1141 tcgtgttata gccccagtag ttgttcaggc ccttttcgac gaggtggcgt tcggacatgc
     1201 tctgatgtac gggcatcagc tccacagagg ttatgcccag ctttttcaga taggcaatga
     1261 cggtaggatg cgccagcgcg gcataggtac ctttgatatc atcggggatg ccgggaaagc
     1321 atttggtaaa gccccgaaca tgcgtttcat aaatgaccgt attgaaatac gccactttcg
     1381 ggggacggtc tcctgcccag tcgaaacgat ggtcgatgac gatcgatttg ggtgcgacag
     1441 ccgcatcgtc acggtcgtcc cggaccaggt catcctgtcc gatagtataa ccgaacagcg
     1501 tatcgtccca atcaccgata ttgcagaacg cccgggcgta gggatcgagc aggatcttgg
     1561 ccgggttgaa ccggtgcccg ttggaaggat cataaggacc gccgacgcga aagccgtata
     1621 gttggcccgg tttcagatcg ggtagatagc catgccagac atcgtgcgtc ctttcgttga
     1681 gtcctatctt ttgcgtctcg cctacgccct tctccgtatc gaacaggcat agctcgacgc
     1741 ccgtggcatt atgtgaataa acggcgaaat tgacgccctt cccgtcccag taagagccaa
     1801 gcggataggg gttacccgga tagattgtca tgatattgtt tttactgagg atttttttcc
     1861 tcagagttcg aatgtgagtt gctggtcatg gcctctttcg ggtatctgcc ggataaaatt
     1921 gtcataaccg gcctcgttat catgactgct gtaggcgcca taggcatcca gactgtaacc
     1981 taattgaccg tcattggctc gaatgacgta tatgatctcc atatcggagg ggtcgctcgc
     2041 accttcgaat cgataggtct ttaagatctc cagctcctgc gggctatagg atttgccttt
     2101 gccgagcgta aatccgccgg gagtccagcg gaattcatcg ccggcgtggt tttggcgcag
     2161 tttctctatt acctgcgagg aagtcgtagc catgctgtcg ggtgcgttca tcatacgaag
     2221 aaagtttaag tgtgtatttc aaatagtgta ccgcagggga aacggagacc aggcaccggc
     2281 ggagtgaggc cgcatcattc gaaccattca atgatacagg cgccgatggc atcgatcagc
     2341 ggctggtcgt ggaggaggtc cattttccag cccgacgcgc cataccggat gatgccgagg
     2401 gtctcgttgt tgagcgtgat atccaactca taacagaagc cttttgcgca ggtctgcgga
     2461 acgggaacgc cttcaccctt gaatcttttc ccctggtatt cgaattcgat ggggatcggg
     2521 tctttttcca gccgccggat gaaatctttc aggaaagcta tcaaatgttt tcgctgggta
     2581 ggcccggagg cgctccattt tcccttgtcg gcccgtttgt gcgcgagctt gaatttcaga
     2641 cccgtcatct tcgtgtcata gtcgttggcg ttgcgtttga ccgcggtctg atgggcgagt
     2701 atataccaat ggtattcggt accgaccgga acccctgaaa aatcgggagc atggcccacc
     2761 ctgcgttttg tcactgcata ggatatttcc caaagatcgg gcgtgatctt ggtctctttc
     2821 caatcgcccg catcgtaatt ccagtgatga ccgtgtccga tctggactcc cgtatacaat
     2881 ttcccgttga attctttgaa tttattgaaa gaggccgaca ggtccttttt agcctttgtc
     2941 tttttgcggg aggaagttcg gggcgttttc ttcgcggtcg ttggagccat aagcgatcga
     3001 attttaccgg tcacccgcag aa
//
LOCUS       DASYSV010000145         9108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_798959, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000145 DASYSV010000000
VERSION     DASYSV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798959"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1081
                     /locus_tag="VGQ51_04530"
     CDS             80..1081
                     /locus_tag="VGQ51_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV9035857.1"
                     /translation="MGIYQDKVVVITGASGGVGRSTAHAFATEGAKMVLLARGKEQLD
                     VTQKEVEALGAMALTIPTDVSDPAQVEAAAEKAEATFGPIDVWVNNAMASVFAPIKEI
                     SPEEFRRVTEVTYLGQVYGAMAALRRMLPRDQGSIVFVGSALAYRGIPLQSAYCGAKH
                     GIEGFYDSLRCELLHDKSKIKTCMVQLPAMNTTQFGWVLSRLPRKPRPMGKIYEPEVA
                     ARAILYAAAHNRRSIWVGAPTYKAIIGNKLSPSYADHVLARIGYKGQQTNEPVSPDRV
                     NNVWEPVSEDRGTHGDFSAMSTNNSFTLWAATHRRWAALAAGIAAVSAGLLIRRVTR"
     gene            complement(1093..3495)
                     /locus_tag="VGQ51_04535"
     CDS             complement(1093..3495)
                     /locus_tag="VGQ51_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV9035858.1"
                     /translation="MDRYSNPAPGAPGQFSNWTTGAKTGVGRALNPGSKVSFTLGKGV
                     LNEVYYPREDTACIRECGFIVSDGVRWFSDERIHTHHKQRSQEPGIPAWLLENRCSQD
                     HYRLGKTIITDPARDCVLQLVRFRPTKKNAQLSLYVYLTPHLQNRGEANEAWLEDYKG
                     MPMLFATGGGLALAMACSCGWRQRTVGFIGASDGYSDIRDHHRLTHQYGYAGKGQVQL
                     CAEPLIGSGHGALKGEEFVLAIGFGHCPEDAAHQARASLLDGFSIAKEQYISEWKEWH
                     HALRSRIKGHAKGKYLKESAAVLRINESRLYPGGIIASLAIPWGEARGVDNGLGYHLV
                     WPRDLVESAWGFLALNAERDVLRILNYLFATQEEDGRWSQNQWLDGQPNLKALQMDQV
                     ALPLLLVDSCYHRGLLDKDRWQRLLPGVRKAIGFILRYGPYTQEDRWEQQPGLSPFTL
                     ATQIAALLGTAALLEESGDTTGAQFCRETADSWNGQIEEWTYVRHTETAKRHGVDGYY
                     IRINPFYAPIQEVKDRRVTIHHHPEHEGDLSIGEVVSVDALALVRFGLRRADDPRILD
                     TIRVIDAELKTELPSGACWHRFTGDAYGEDEAGNPFVVTGIGRCWPLLTGERAHYEIA
                     AGHLRDAGQLVKAMEGFSWHGLLPEQVWDKDDIPEKGLFKGKYTGSAMPLTWAQAEYI
                     KLAISLRHEKVFDIPPATVKRYSKKEHHSHLLIWRFDRPMLGAADRVSKTHLRVELFD
                     RACVRWSMDGWATNDELETTDTGLGIHYADVPLPSGGRLMFTFYWKEAGHWEGKDFAV
                     EI"
     gene            complement(3501..6626)
                     /locus_tag="VGQ51_04540"
     CDS             complement(3501..6626)
                     /locus_tag="VGQ51_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEV9035859.1"
                     /translation="MDKGSAYVPHAHSHNKYELFQENSYYDTRYVTPQSENVDVAALE
                     DDLKSAQVGEVRFDPGSKATYSTDSGNYRQIPLGVVVPRTQRDIEEIIAICHRHKAPV
                     VNRGGGTSLAGQTCNVAVVIDMSKYYNRVLVLDKEGKTVTVQPGIVLDHMKAYTEQHG
                     LTFGPDPSTHNHCTIGGMLGNNSCGVHSIMSANYGYGAKMEHNITTMTVLTYDGIKMT
                     VGPTSDEEFARIVAGGGRKAEIYMKLRALVDKYADQIRERFPDIPRRVSGYNLPDLLP
                     ERGFNVAAALVGSESTCVTILSATCRLMPTPKARSLVVLGYPDLYESGKHVKEILAFK
                     PIGLEGLDDLLIQNMQRKGYHTEHLTLLPGGKAWLLVEFGGDSKTESDELAHAMMAKL
                     KARKDPPEMNLFDDPKQEEMIWKIRESGLGATAWVPGDPITEEGWEDSAAPPDKVGEY
                     LVALRKLFEKYGWANVPLYGHFGQGCVHCRIPFDLQSPEGLDQYKRFTEEAAHLVVSF
                     GGSISGEHGDGQSKADLLGIMYGPELVQAFREFKEIWDPDWRMNPGKITEPYGQLRNL
                     RTGAQYQPAEPVTHFQYPDDIGRFSRAALRCVGIGECRRTSGGTMCPSYRVTKEEEHS
                     TRGRARLLYEMLMGDVVDGWKDKNVKNALDLCLSCKGCKGDCPVNVDMATYKAEFLSH
                     YYKGKVRPRSAYAMGFIYWWARLASVTPRVANFFTQTPGLRSISKWIMGVAPQRQIPA
                     FATQTFKDWFRRRTGPAYYRPLGDRMRPGERPRVILWADTFNNNYFPETLKAAVTVLE
                     AAGFSVAVPGASLCCGRPLYDYGLLTTAKKLLRKILDTLREDIRNGIPVVGLEPSCIA
                     VFRDELTNLFPTDKDAVRLKTQTFTLAEFLQKKAPNFRPPLLKHEALVHGHCHQKAIM
                     KMDAEKELFEKMKLDFEIVDSGCCGMAGSFGYEKGTHYTVSVQVGELVLLPAVRNASR
                     DAFIIADGFSCREQIIQQTDRTPMHTAEVIALALRQGQTSVVAQPSMVTQPASTSEPI
                     SNKKEISQHED"
     gene            complement(6681..7808)
                     /locus_tag="VGQ51_04545"
     CDS             complement(6681..7808)
                     /locus_tag="VGQ51_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEV9035860.1"
                     /translation="MNTLVKPGAGRVTDHRISDVQVTAYTLPTDRPESDGTLEWQETT
                     IIVVRVRSADKEGLGYTYGHVAIVDVIRSLLAPIVMDRQPMDVMALQTAMIHHVRNNG
                     QAGLAMMAVSAVDMAIWDLKARLLGLPLCRLLGQEHESMLIYGSGGFTSYTNAELRQQ
                     LEGWAEQGLVAVKIKVGRDMEADKTRIGVARDAIGEKIGLFVDANAAYSVSEALDQAR
                     LFSGYGVDWLEEPVPAQSREQLNLIRQRLDGGMRVVAGEYGYTLNDFRDLLEAGAVDV
                     LQADATRCGGITGFLKAGHLSEAWHVPLSSHCAPSAHLHAALSLPVFSIGEYFHDHVR
                     IEKLLFDGAAVPQDGVIFPDMGRPGLGLIFLDSKAEPYRCA"
     gene            complement(7821..8165)
                     /locus_tag="VGQ51_04550"
     CDS             complement(7821..8165)
                     /locus_tag="VGQ51_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEV9035861.1"
                     /translation="MPFSVYRDCIDACTHCAEMCHQCADACLEEDDVHSLRTCIRLDL
                     ECAAACRTAVEMMQLDGNFTGAYCELCADVCDACAEECTKHAGKGMEHCRICAEACRQ
                     CSEECSKMASMA"
     gene            complement(8181..>9108)
                     /locus_tag="VGQ51_04555"
     CDS             complement(8181..>9108)
                     /locus_tag="VGQ51_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV9035862.1"
                     /translation="FLPKEGAAKAVQIDIDGRMLGLRYPTDVNLQGDSRRTLKALIPL
                     LVRKEDRTWREKIESEITRWWKVLEARAMKSANPINPQRFFWELSPLLPDNCILTSDS
                     GSTANWFARDLRVRKGMKASLSGNLATMTPGVPYAIAAKFAFPDRIPIAMVGDGAMQM
                     MGMNELITISKYYRRWSDPRMLIIVLNNQDLNQVTWEQRVMSGDPKYEASQDLPGLNY
                     AAYAQLLGLEGIRIEKEDEIGPALQTALNARRPVVLDLLSDPNVPPLPPHITLKQAKD
                     FSSSILKGDVNAWDMIKQTYKEVVENFLPHHS"
BASE COUNT         1875 a         2622 c         2521 g         2090 t
ORIGIN      
        1 tgtcgggggg gtcctggcga aggcggcctc ttttttgtcg ggcgatgacc atggcattct
       61 tttttctttc ggataagata tgggaattta tcaggacaag gtagtggtca tcaccggcgc
      121 cagcggcggg gtgggacgtt caacggcgca cgcgtttgcc acagagggcg ctaagatggt
      181 gttattggcc agaggcaagg aacagctgga tgttacgcaa aaagaagtgg aggcgctggg
      241 ggccatggcg ctgaccatcc ccacggatgt atcggatccg gcacaggtgg aggcggcggc
      301 ggaaaaggcg gaggcgacgt tcggacccat cgacgtgtgg gtaaacaatg ccatggccag
      361 cgtgtttgca cctataaagg agatcagtcc ggaggaattc agacgtgtca ctgaagtgac
      421 ctatctcggt caggtgtatg gcgcgatggc agcgcttcgc cggatgttgc cccgcgacca
      481 gggttcgatc gtgttcgtcg gctcggcatt ggcctatcgc ggcattcctt tgcagtccgc
      541 ttattgcggc gcgaaacatg gcatcgaggg attctacgat tccctgcgat gcgagttgct
      601 gcatgacaaa agcaagataa agacctgcat ggtacagctg cccgcaatga ataccacaca
      661 attcggttgg gtgctgagcc ggcttccccg aaaaccgcgt ccgatgggaa agatatatga
      721 acccgaagtg gcggcccgcg ctatcctgta tgcggccgcg cacaaccgcc gttctatctg
      781 ggtgggggcg ccaacatata aggcgatcat cggaaacaag ctgtcgccat cctatgccga
      841 ccacgttctt gcgcgcatcg gatataaagg ccagcagacg aatgagccgg tctcgcccga
      901 cagggtcaac aatgtgtggg agccggtgag cgaggatcgc ggtacgcatg gagatttttc
      961 ggcaatgtct accaataaca gttttacttt gtgggccgcc acgcatagaa gatgggctgc
     1021 gctggcggcg ggtatcgcgg ctgtttcggc aggattgctg atccggcggg tgaccaggtg
     1081 acgcgaatgc ctttagattt cgaccgcaaa atcctttcct tcccaatgcc ccgcttcctt
     1141 ccaataaaag gtaaacatca atcggccgcc ggagggcaat ggtacatcgg catagtggat
     1201 cccgagccct gtatccgtcg tctccaattc gtcattggtg gcccatccgt ccatactcca
     1261 gcgaacacag gcccggtcga acagctctac ccgtaaatgc gtcttcgaaa cgcggtcggc
     1321 cgcgcccagc atcggacggt caaagcgcca gatgagcaga tgggaatggt gttctttttt
     1381 ggaatatctt tttaccgtcg ccggagggat gtcgaatacc ttttcgtgtc gtagcgatat
     1441 ggcaagcttg atatattccg cctgcgccca ggtcaacggc attgccgatc cggtatactt
     1501 gcctttgaag agtccctttt ccggtatatc gtccttgtcc cagacctgtt cgggcagcag
     1561 cccatgccag ctgaacccct ccatggcttt gaccaattgg ccggcatcac ggaggtgccc
     1621 cgcggcgatc tcataatggg cacgttcgcc ggtgagcagt ggccagcagc gccctatgcc
     1681 tgtaactacg aatggattac cggcctcatc ctccccgtat gcgtcgccgg taaatcgatg
     1741 ccagcatgca ccggacggaa gctctgtctt tagttccgcg tcgatgaccc ggatagtatc
     1801 gaggatgcgt ggatcatcgg cccggcgtaa gccaaaccgt acaagcgcga gcgcgtcgac
     1861 gctgactact tcgccgatgg acagatcgcc ctcgtgttcc ggatgatggt gaatggtgac
     1921 gcgacggtcc tttacctcct gtatcggagc atagaacgga ttgatacgga tataatagcc
     1981 gtcgacgcca tggcgtttag ccgtctccgt atgcctgacg tacgtccact cttcgatctg
     2041 cccgttccag ctgtcggccg tctcgcgaca aaattgcgcg ccagtggtat cgccggactc
     2101 ttccagcagg gcggccgttc ccagcaaagc ggctatctgg gtggcgagcg taaagggaga
     2161 gaggccgggc tgttgttccc accggtcctc ctgcgtgtag gggccatacc gcaaaatgaa
     2221 gccgatggct tttcgtactc ccggcagcaa ccgctgccag cggtccttat caagcagccc
     2281 gcgatgatag caggagtcga cgagtaacag cggcagcgcc acctggtcca tctggagagc
     2341 cttcagattc ggctggccat ccagccattg gttctgactc catctgccat cctcctcctg
     2401 tgtcgcaaaa agatagttca ggatccgcag gacatcccgt tcggcattca gggcgaggaa
     2461 tccccaggcg ctctccacca ggtctcttgg ccatacgagg tgatatccca gtccgttgtc
     2521 tacccctctt gcttcccccc agggtattgc gaggctggcg atgatgccgc cgggataaag
     2581 ccggctctca ttgatgcgta atacggcggc gctttccttc aggtatttgc cctttgcatg
     2641 cccttttatc ctcgagcgga gagcatggtg ccattccttc cattccgata tgtattgttc
     2701 tttggcgatc gaaaacccgt cgagcaggct tgccctcgcc tgatgtgccg catcttccgg
     2761 gcaatgcccg aacccgatgg cgagaacgaa ttcttcgcct ttgagggcgc cgtggcccga
     2821 tccgatcagc ggttcggcgc atagctgcac ctgacccttg cccgcatatc cgtattgatg
     2881 tgtcagccgg tgatgatccc ggatatcgga atatccgtcg gatgcgccta taaaaccaac
     2941 agtccgctgg cgccacccgc agctgcaggc catggccagc gccagaccgc cacccgttgc
     3001 gaacagcatc ggcatcccct tgtaatcctc cagccaggct tcattggcct cgccgcgatt
     3061 ttgcagatgg ggagtaagat agacatacag cgaaagctgc gcattctttt tcgtcggccg
     3121 gaaccgcaca agctgcagca cgcagtcgcg cgccggatcg gtaatgatcg tcttcccgag
     3181 ccggtagtga tcctgcgaac agcgattttc gaggagccac gcgggtatcc cgggctcctg
     3241 tgagcgttgt ttatgatgcg tatgtatcct ctcatcggaa aaccagcgga cgccgtcgct
     3301 cacgatgaaa ccgcattccc tgatgcaggc cgtgtcctcg cgtggataat agacctcgtt
     3361 gagcacacct ttgccgagcg tgaagctcac cttcgaccct gggttcagtg cccggccgac
     3421 acctgttttg gcgcctgtag tccagtttga aaactgcccc ggtgcacccg gggcagggtt
     3481 gctgtatcga tccatataca tcagtcttcg tgttgactga tctctttttt atttgaaata
     3541 ggctcgctgg tggatgcggg ttgcgttacc atcgagggtt gcgctaccac cgaggtttgt
     3601 ccctgtcgca atgccagcgc aatcacctcc gcggtatgca tcggtgtgcg gtccgtctgc
     3661 tggatgatct gttcgcggca gctgaatcca tcggcgatga tgaacgcgtc acgcgaggcg
     3721 tttcgcacgg caggcagcag cacaagctca cccacctgca cacttacggt atagtgcgtg
     3781 cctttctcat agccgaacga gcctgccatt ccgcaacaac ccgagtccac gatctcgaaa
     3841 tccagcttca ttttctcgaa caattccttt tcagcatcca ttttcatgat cgccttctga
     3901 tgacagtgac catggaccag cgcctcatgt ttcaacaaag gcggacggaa gttcggcgcc
     3961 tttttctgca ggaactccgc aagcgtgaag gtctgtgtct tcagacgcac ggcatctttg
     4021 tcggtgggga agaggtttgt cagctcatcg cggaataccg ctatacagct cggctccaga
     4081 cccacgacgg gtatgccgtt acggatatct tcacgcaggg tgtccagtat ctttcgcaat
     4141 agttttttgg cagtggtgag cagaccgtag tcatacaacg gccggccgca gcaaagcgag
     4201 gcgcccggca ctgcgacact gaaaccggcg gcttccagta cggtaacggc ggccttcagc
     4261 gtctccggga agtaattgtt gttgaatgta tccgcccaca ggatgacgcg cggccgttca
     4321 ccgggtcgca tacggtcacc caacggccgg taatatgccg gtccggtccg gcgtctgaac
     4381 caatccttga atgtctgtgt ggcaaaggcc ggtatctgtc gctggggcgc cacgcccatg
     4441 atccatttac tgatgctgcg aagtccggga gtttgtgtaa agaaattcgc cacacgcgga
     4501 gtcaccgagg ccagtctcgc ccaccaatag atgaacccca tggcataggc agaacgcggt
     4561 ctgaccttcc ccttataata atgtgaaagg aattcggctt tataggtggc catatcgaca
     4621 ttcaccgggc aatcgccttt acagcccttg caggacagac agagatccag ggcatttttg
     4681 acatttttat ctttccagcc atcgaccaca tcgcccatca gcatttcata caaaagacgc
     4741 gcgcggccgc gggtagaatg ttcttcttcc ttcgtgacgc gatagcttgg acacatcgtc
     4801 cctccgctgg tcctgcggca ttcgccgatg cccacgcaac gcagcgcggc gcggctgaat
     4861 cttccaatgt catctggata ttgaaaatga gttaccggtt cggccggctg atattgtgcc
     4921 cccgtccgca gattgcgcag ctgaccatag ggctcggtga tcttacccgg attcatacgc
     4981 caatcgggat cccagatctc cttgaactcc cggaatgcct gaacgagctc ggggccgtac
     5041 atgatcccga gcagatccgc cttcgactga ccgtcaccgt gttcacccga gatgcttcct
     5101 ccgaaactga ccacgagatg ggcggcctct tccgtaaacc gtttgtattg atccaggcct
     5161 tcgggggact gcagatcgaa cggtatgcgg caatggacgc agccctggcc gaaatggcca
     5221 tatagcggaa cattcgccca gccgtatttt tcaaaaagtt ttcttaacgc cacaagatat
     5281 tcgccgacct tatcgggcgg cgccgcggag tcttcccatc cttcctcggt aatgggatcg
     5341 cccggcaccc aggcggtggc gccgagaccc gattcgcgaa ttttccatat catctcctcc
     5401 tgcttagggt cgtcgaaaag attcatttcc ggcggatctt ttcttgcttt cagtttggcc
     5461 atcatcgcgt gagccaattc gtcggattcc gtcttgctat cacctccgaa ttccaccagc
     5521 agccaggcct tgccgcccgg caataatgtc aaatgctccg tgtgataacc tttccgctgc
     5581 atgttctgga tcagaagatc gtcaagacct tccaacccga tgggtttaaa agccaatatc
     5641 tccttcacat gtttgccgct ctcgtataga tcaggatagc cgagcaccac cagcgaccgt
     5701 gcctttggcg taggcatgag cctgcaagtc gcgctcagga tggtcacgca ggtgctttcg
     5761 ctgcctacca gcgccgcggc gacgttgaaa cctctttccg gcaacagatc cggcagattg
     5821 taacccgaga cacgacgggg tatatccggg aagcgttcgc ggatctggtc ggcatatttg
     5881 tcgaccagcg ccctgagttt catatagatc tcggcttttc gtccgcctcc cgccacgata
     5941 cgggcaaatt cttcatcgga cgtaggtccg acggtcattt tgatcccgtc ataggtgagc
     6001 actgtcatcg tggtgatatt atgctccatc ttggcgccat agccataatt ggcggacatg
     6061 atgctgtgta caccgcagga attattgccc agcatgccgc cgatggtgca gtggttatga
     6121 gtggacgggt cgggaccgaa agtaagaccg tgttgctcgg tatacgcttt catgtgatcg
     6181 aggacgatgc ccggctgtac ggtgacggtc ttgccttctt tatcgaggac gagcacccgg
     6241 ttatagtatt tggacatgtc tatgacgaca gccacattgc aggtctgacc ggccagactc
     6301 gtaccgcctc cgcggttgac gaccggcgcc ttatggcggt gacagatggc aatgatctct
     6361 tcgatatcgc gctgcgtgcg cggcactacg actcccaggg gtatctgacg gtaattgccg
     6421 gagtcggtgc tgtaggtggc cttgcttccc ggatcaaaac gtacctcgcc aacttgcgcc
     6481 gacttcagat cgtcttccag cgccgccacg tcgacgtttt ccgattgagg tgtgacataa
     6541 cgtgtatcgt aatagctgtt ttcctgaaag agctcgtatt tgttgtggct atgagcgtgc
     6601 ggcacgtaag cagatccctt atccatctta ttggaattta agagtgtacc atttagtatg
     6661 gaaatattat gccatccggt ctatgcgcaa cgataaggct ccgccttgct gtcgaggaat
     6721 atcagaccaa gaccggggcg gcccatatcg ggaaaaatga ctccatcttg tggtactgcc
     6781 gcaccgtcga agaggagttt ttcgatccgg acgtggtcat gaaaatattc gccaattgaa
     6841 aagacgggta gggacagcgc cgcatgcaga tgtgcggatg gagcacaatg agaagacagc
     6901 gggacatgcc atgcctcgga gagatggccg gccttaagaa aacccgtaat acctccgcaa
     6961 cgtgtggcgt ctgcctgcag cacatccact gctcctgcct ccagcagatc gcgaaagtca
     7021 ttaagagtat agccgtactc acccgcgact acgcgcattc caccatcgag tcgctgccgg
     7081 atgagattca attgttcgcg tgattgtgcc ggcacgggtt cttccagcca gtctacgccg
     7141 tagcccgaga acagccttgc ctggtcgagc gcctcgctga cggaataggc ggcgttggcg
     7201 tcgacaaaga ggccgatttt ttcaccgatg gcatcccgcg cgacaccgat acgggtttta
     7261 tcagcctcca tgtctctgcc aacctttatc ttgactgcaa caagcccctg ttcggcccat
     7321 ccttccagtt gctggcgcag ctccgcatta gtataggagg taaaccctcc gctgccgtag
     7381 atgagcatgg actcatgttc ctgtcccaat agccggcata agggaagacc gagcaaccgg
     7441 gctttaagat cccagatggc catatcgacg gcggataccg ccatcattgc gagacccgcc
     7501 tgaccgttgt tgcggacgtg atggatcata gccgtctgga gggccatgac gtccatcggt
     7561 tgcctgtcca tgacgatggg agcaagtaat gatctgatca catcgacgat ggcgacatga
     7621 ccataggtat agcccagccc ttccttatcc gcggacctga ccctgacgac gatgatcgtc
     7681 gtttcctgcc attccagcgt accatccgac tcaggacgat cggtcggtag cgtgtaggcg
     7741 gtgacctgca cgtccgatat ccggtgatcg gtcaccctcc cggcgcccgg ttttacgagt
     7801 gtgttcataa atatttccat tcaggccatc gaggccattt tactacattc ttcggaacat
     7861 tgacggcagg cttccgcgca gatgcggcaa tgttccatgc ctttgcccgc atgtttggta
     7921 cattcttcgg cgcaggcgtc gcatacatcc gcacagagct cgcaataggc cccggtgaaa
     7981 tttccgtcca gctgcatcat ctcaaccgcc gtgcggcagg cagccgcgca ctccaggtcc
     8041 agccggatac aggtgcgcag ggaatgtaca tcgtcctctt ccagacaggc gtccgcacac
     8101 tgatggcaca tctccgcaca gtgggtgcag gcatcgatac agtcccgata aactgagaag
     8161 ggcatagcca tgatttattt ttatgaatga tggggtaaga aattttcgac gacttcctta
     8221 tacgtctgtt tgatcatatc ccaggcattg acgtctccct tcaggatcga ggagctgaaa
     8281 tccttcgcct gtttgagcgt aatatggggc ggcaatggcg ggacgttcgg atcactgaga
     8341 aggtcgagca ctacgggccg ccgggcgttc aatgccgttt gcaatgccgg accgatctcg
     8401 tcctcttttt cgatgcggat accctcgaga ccgagcagct gcgcgtaagc agcatagttc
     8461 agcccgggaa gatcctgtga tgcctcgtat ttaggatcgc ccgacatgac acgttgttcc
     8521 catgtcacct ggttgaggtc ctggttgttc agcacgatga taagcatacg cgggtcggac
     8581 cagcgacgat agtatttgga gatggtgatc agctcgttca tgcccatcat ctgcatcgcc
     8641 ccgtccccta ccatggcgat gggaatgcga tcggggaagg caaacttcgc ggcaatagca
     8701 taaggaacac caggggtcat cgtggccaga ttgcccgaca acgaggcctt catgcccttc
     8761 cggacgcgaa ggtccctcgc gaaccagtta gccgtcgatc ccgagtccga tgtcaggata
     8821 caattgtccg gcagaagggg cgatagttcc cagaagaaac gctggggatt gatgggattg
     8881 gcgcttttca tcgcgcgcgc ctcgaggacc ttccaccatc gggtgatctc cgactcgatc
     8941 ttttcgcgcc aggtgcggtc ttcttttctg acaagcaatg gtatcagcgc cttcagcgtc
     9001 ctgcggctgt cgccttgaag atttacgtct gtaggataac gcagccccag catccttccg
     9061 tcgatatcga tctggaccgc ctttgccgcc ccttctttcg gaaggaat
//
LOCUS       DASYSV010000146         8600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1051771, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000146 DASYSV010000000
VERSION     DASYSV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051771"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VGQ51_04560"
     CDS             <1..286
                     /locus_tag="VGQ51_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEV9035863.1"
                     /translation="GNKVVEVRIAGYDKGATALRIVGESDPDFVLCMGDDTTDEDMFK
                     ALDGEAYTIKINNGASAAQYTILSQQQVLPLLNRITSESSVRSEKEYAGS"
     gene            273..2051
                     /locus_tag="VGQ51_04565"
     CDS             273..2051
                     /locus_tag="VGQ51_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV9035864.1"
                     /translation="MPVHKYDMGVIGNCSYLAYIDTRADVKWMCLPRFDSSFLFGSLL
                     DEDNGGHFYIRPTMDNCSSRQYYIPNTNVLCTEFTCPDGKFVVTDCAPRMRIYERQFR
                     PLMMVRKIHLLEGTPAVTICCQPRGDYGRVVPESVIASNHIRYLNLDQQVRLTTDVPL
                     NYVQKEKSFILDQDRYLVLTYGEPLEAPLKETAEEFIRKTVEDWQSWIKSSYLPDIYQ
                     EQIIRSALVLKLHQYEDTGGIIASGSTSLPEFHDSTRNWDYRYCWFRDAHYTLRAFNQ
                     IGHFEELEKYFDFIYNILHHATDALQPLYSVSGDQKLYEITLDLEGYLGNKPVRIGNK
                     AYIQSQHDVYGQVLVSLLPLYTDKRLLFLKKASYRKIVPWLLAQIERTLEMPDSGLWE
                     FRNAEQVHTYTLLFHWAGAKAAYKIGHHFGDQDLMRKAHRLVEAADQLIAKTYDPGRK
                     LFTQAIGTANLDASTLSMITMDYLDHRGERAKEHIAALEKELLTPKGLFYRYKHYDDF
                     GFPESTFLVCAFWYVDALACVGRVDDALRHLDQLLGFANHLGIFSEDAIDDGSQWGNF
                     PQTYSHVGLINAACRISRKLDKADFL"
     gene            2068..3321
                     /gene="hutI"
                     /locus_tag="VGQ51_04570"
     CDS             2068..3321
                     /gene="hutI"
                     /locus_tag="VGQ51_04570"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEV9035865.1"
                     /translation="MSPHLFYNIRQLVNTREDSPLLRGGELAELPVIHDAWLHIEGEH
                     IAGFGPMSELPPALASLRGTSDGTDASGRLVLPAWCDSHTHLVYAGSRESEFVDKIRG
                     LSYADIHAKGGGILNSARRVAETPEEVLFTEAWQRLQELVKMGTGAVEIKSGYGLTVE
                     GELKMLRVIRKLKQRSKLAIKATFLGAHAYPERYRQDHEGYIDLIVNEMLPAIAAGGL
                     ADYIDVFCEEGFFSPAETETVLRAGARYGLPAKIHANQLHLSRGVETGVRLGALSVDH
                     LETMDSSAIAVLAGSGTIGTLLPTAAFFLRMPYQPARALIDAGCAIALASDYNPGSSP
                     SGNMNLVVSMGCIQMRLLPEEAINAATLNGAHAMSLGDRIGSITRGKLANLIVTRPVP
                     SLAYLPYSFGSDLVERVMIRGEFLR"
     gene            complement(3326..3535)
                     /locus_tag="VGQ51_04575"
     CDS             complement(3326..3535)
                     /locus_tag="VGQ51_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035866.1"
                     /translation="MLKSKLTLYAVIASFFFLGVYEKKMGEVLDIPTSNAPKPEYMSI
                     SGNILIFIAIGLFLLCSYTLDKKKG"
     gene            3622..5265
                     /gene="ftcD"
                     /locus_tag="VGQ51_04580"
     CDS             3622..5265
                     /gene="ftcD"
                     /locus_tag="VGQ51_04580"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEV9035867.1"
                     /translation="MDSLIECVPNFSEGRRPEIIKQITDVIEGVPGVRLLNVDPGKAT
                     NRTVVTFVGAPAAVIEAAFQAIRKAGELIDMRDHKGEHPRMGATDVCPLIPIAGITME
                     ETADWARRLASRVGTELRLPVYLYEEAQPDKRRSNLSIIRAGEYEGYFKKIALPEWKP
                     DFGPAEFDARRGGTVIGARDFLVAYNVNLNTTSTRRANAIAFDVREAGRTLPDGTHQP
                     GTLKSVKAIGWFIEEYGVAQISMNLTNIRITPVHVAFDEVARAASARGIRVTGSELVG
                     LVPLQAMLDAGRYFLEKQQRSTGVSEKELIRIAILSMGLDELAPFVPEERIIEYLLRG
                     SADRRLVGMSLLDFADETASESPAPGGGSIAAYVGALGAALGTMVANLSSHKKGWDDR
                     WTLFGGWAERGQGYKDELLRLVDADTRAFNSIMAAFGMSKGTAEEKALRHQAIQAATR
                     TAIEVPFRVMEVAAASMEVIRAMALDGNPNSVSDAGVGALCVRAAVMGAFLNVRINAA
                     GYEDKEFVKDIHDRGSRIMAEANKAETEILNIVLGKMGS"
     gene            complement(5307..7592)
                     /locus_tag="VGQ51_04585"
     CDS             complement(5307..7592)
                     /locus_tag="VGQ51_04585"
                     /inference="COORDINATES: protein motif:HMM:NF039873.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV9035868.1"
                     /translation="MKNYPLLSFCVFFLLAGGRLSAQDSSASQSPAMQSQTAIRSQRP
                     KIGLVLSGGGAKGLAHIGILKAIDSAGLKVDYITGTSMGSIIGGLYAVGYSADSIEKI
                     AGSIDWDLLLSNQSSMRSMIMDEKDEYGKYIVELPWVNHWFRINTGVIEGEELWMKFS
                     ELFRPVYNIKDFSKFSIPFRCIATDISTGEAVVLDTGEIVPALRSSMAIPTIFTAVQY
                     GGHKLVDGGVVRNFPVSDVRKMGADIVIGDNVSGPLRTPDQVTNPLQVLMQVAFFREA
                     ADTRTQIPLCNIYVYQPLDKYNMGNFGEAGEIIETGLKAGRLLYPRLKKLVDSLDATY
                     GPQQTVKNRLPAVYPVRISSYEVTGLKHTSEAFFIQSINLESGHSYSATALSKMVRRA
                     FGTRYYSRITYSLTPQPDSSSHIKFEVEETPLTFAKLGLNYNQFSGISAILNLTTRDF
                     FTPTSRSLVTLNIGQNFRARAEHLQYFSRRANFSFALATQFDEFRITTYNDKYQEAGL
                     YDQNYFKLDGRFGYSTNRDLTVGVGSRWEYTDYAPSITSSLEFEGNNNFLTSYFFVRS
                     NTLDRPIYPRRGFRFHVEADHVFEQSPEAQYFASTVNRSDTSFSEEPYERLLANYDQF
                     IPVTNRYTVQVHLQGGLNLNYHHNIMNEFSIGGLTDQFHNQVVFAGLREGTFYSASVA
                     EGMLALRYQLFNNVMVTGKANILFNNFLDKSSFFTTPDFLSGYALTFTYRFALGPLEL
                     SAMYCDQSRRVIGYVNIGIPF"
     gene            complement(7589..>8600)
                     /locus_tag="VGQ51_04590"
     CDS             complement(7589..>8600)
                     /locus_tag="VGQ51_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9035869.1"
                     /translation="ILASLKGASKHFGPITIVENASAEIDRGDKIALIGANGKGKSTL
                     LRMVAGTEPFEGQRVWGHNVEESFYAQHQLESLNVNNTLLEEMQLCGSQKTDLELRVL
                     LGCFLFSGDDVEKKIRVLSGGEKARVALAKTIISKANFLLLDEPTNHLDMHSCDLLIE
                     ALTRYEGSYLLVSHDRYFISRTANKIWEIEDGLIREFKGTYEEWVDWKERNLAAKQQA
                     AVAAVPVAVAAPPPPPPVPGPPSDRPRPSDLGARPNKEAKKELQRQQRLFQDLEQKIA
                     TLTRQKDALEASLADPATYSDKNKFIKAETDYKKTSDELDKANRQYEQVFEKIMELEK
                     TL"
BASE COUNT         1855 a         2227 c         2567 g         1951 t
ORIGIN      
        1 cgggaacaag gtcgtcgagg tgcgcatagc gggttacgac aagggggcga ccgccttgag
       61 gatcgtcggc gaatcggacc cggacttcgt cctgtgcatg ggtgacgata ccaccgatga
      121 ggacatgttc aaggcgttgg atggcgaggc ctataccata aagatcaata acggcgcttc
      181 ggcggcgcaa tatacaattc tttcccaaca acaggtgctg ccgctgttga acaggatcac
      241 cagcgaatct tccgtccgat cagaaaaaga atatgccggt tcataaatac gacatggggg
      301 ttatcgggaa ttgctcgtac ctggcttata tcgataccag ggcggacgtg aaatggatgt
      361 gtctgcccag atttgacagc agctttcttt tcgggagcct gctcgatgag gacaacggcg
      421 gccacttcta tatccgtcct acgatggaca actgcagctc ccggcagtat tatatcccca
      481 acaccaatgt tctctgcacc gagttcacct gccccgacgg aaaattcgtc gtcaccgact
      541 gcgccccgcg gatgcgcatc tacgagaggc agttccggcc gttgatgatg gtgcggaaga
      601 tacaccttct ggagggcacg cccgcagtga ccatttgttg tcaaccgaga ggggattatg
      661 gcagggtggt accggaatcg gtgatcgcca gcaaccatat ccggtatctc aacctcgatc
      721 agcaggtgcg tctgaccacc gatgtaccgc tgaattatgt ccagaaggag aaatccttta
      781 tcctcgatca ggaccgctac ctggtactca cctatggcga gccgttggag gcgccgctga
      841 aggagaccgc ggaggagttc atccgcaaga cggtggaaga ctggcaatcc tggatcaaga
      901 gctcgtacct tccggatata taccaggaac agatcatccg gagcgcgctg gtcctcaagc
      961 tgcatcaata tgaggatacg ggcgggatca tcgcctcggg atcgacgagc ctcccggaat
     1021 ttcacgacag cacccgcaac tgggactacc gttactgctg gttccgggat gcgcactata
     1081 cgcttcgcgc ttttaaccag atagggcatt tcgaagagct ggaaaaatat ttcgatttca
     1141 tctataacat tctccatcac gccaccgacg cccttcagcc cttgtattcg gtctcgggcg
     1201 atcaaaagct ctacgagata accctcgatc tggaaggcta tctgggcaac aagccggtgc
     1261 gtatcgggaa taaggcctat atccagtctc agcatgacgt atatggacaa gtgctggtga
     1321 gcctgttgcc gttgtatacc gacaagcgtc ttctttttct caaaaaggcc agctaccgga
     1381 agatcgttcc ctggttgctt gcgcagatcg agcggacctt agagatgccg gattccggat
     1441 tgtgggagtt ccgcaatgcc gaacaggtgc atacctatac cctgttattc cattgggcgg
     1501 gagccaaggc ggcctacaag atcggacatc acttcggcga ccaggacctg atgcggaagg
     1561 cccatcggct ggtggaagcc gcggaccagc tgatcgcgaa gacctatgac cccgggcgaa
     1621 agctctttac acaggccatc ggtacggcca atctcgacgc gagcaccctt tccatgatca
     1681 cgatggacta tctcgatcat cgcggagaac gggcgaagga gcatatcgcg gcactcgaga
     1741 aggagctgct gacaccgaag ggactttttt accggtacaa acactatgac gatttcgggt
     1801 tcccggaaag tacctttttg gtctgcgcct tctggtatgt agatgcattg gcctgtgtcg
     1861 gtcgcgtcga tgacgccctc cggcatctcg atcagctatt ggggtttgcc aatcacctgg
     1921 gcatcttcag tgaggacgcg atcgatgacg gatcgcaatg gggcaatttc ccgcagacct
     1981 attcgcatgt agggttgatc aatgccgcat gccgaatatc gcgaaagctg gacaaggccg
     2041 attttttata attccctatc ttcgggaatg tccccgcatc ttttttacaa cattcgtcaa
     2101 ttggtgaata cccgggagga cagccctctg ctccggggag gcgagctcgc cgaattaccc
     2161 gtcatccacg atgcctggct gcatatcgaa ggggaacata tcgcaggctt cgggcccatg
     2221 tccgaattgc caccggcgct tgcgtcgttg cgcggtactt cggacggtac agacgccagt
     2281 ggtcgtctgg tgctgcctgc atggtgcgac agtcatacac atcttgttta tgccgggagc
     2341 agggagtccg aattcgtgga caagatccgg ggtctgagct atgccgacat ccatgcgaag
     2401 ggtggcggga tcctgaactc cgcccggcgt gtagccgaga ccccggaaga ggtgttattt
     2461 accgaggcct ggcagcgtct gcaggagctg gtgaagatgg gtaccggcgc cgtcgagatc
     2521 aagagcggct atggcctgac ggtggaaggg gagttgaaga tgcttcgggt catcaggaag
     2581 ctgaaacaaa ggtcgaaact ggcgatcaag gccaccttcc tcggcgcgca cgcatatccg
     2641 gaacggtacc ggcaggatca tgaaggctat atcgatctga tcgttaacga aatgcttccg
     2701 gctatcgccg ccgggggtct ggctgactat atcgatgtat tctgcgaaga aggttttttc
     2761 agtcccgccg aaacggagac tgtcttacgt gccggcgctc ggtacgggtt acctgccaag
     2821 atccatgcga accagctgca tttatccagg ggcgtcgaga cgggagtcag gttaggggca
     2881 ttatccgtcg atcacctcga gaccatggac tcttcggcga ttgccgtcct ggctggatcc
     2941 ggaacgatcg gtaccctgtt gcctaccgcc gccttctttc tcaggatgcc ttatcagcca
     3001 gcccgcgccc tgatcgacgc gggatgcgcc attgcgctgg catcggacta taaccccgga
     3061 tcgtctccca gtggcaatat gaatttagtc gtgtcgatgg gctgtatcca gatgcggctt
     3121 ctcccggagg aggcgatcaa cgcggccacc cttaatggcg cccatgcgat gtcgctgggt
     3181 gacaggatcg ggagcatcac tcgtggcaaa ttggccaacc tgatcgttac gcggccggtg
     3241 ccttcactgg cgtatcttcc ttattccttc ggcagcgatc ttgtagaaag ggtgatgatc
     3301 cgcggggaat tcctgcgatg aggtgtcaac ctttcttttt gtcgagagta tagctgcaaa
     3361 gaaggaacag gccgatggca ataaagatca gtatattccc gctgatgctc atgtattcag
     3421 gcttaggagc attactggta gggatatcaa ggacttctcc cattttcttt tcatatacgc
     3481 cgaggaagaa gaatgacgct atcacggcgt ataaggtgag cttggatttt agcataaacg
     3541 tcgattcgca cgcaaaatta gttaatttgg gaacggaaac gtaaatcaac gatcgtattg
     3601 aataaataat tgaaaaatta catggattct cttattgaat gcgtccccaa tttcagcgaa
     3661 ggccggcgtc ccgagatcat caaacagata acggatgtca tcgaaggtgt tccgggagtc
     3721 cgtttattga atgtcgatcc tggcaaagcg acgaaccgga cggtcgtgac gttcgtgggg
     3781 gcgccggctg cggtcattga ggcggcgttt caggctatcc gaaaggcggg agagttaata
     3841 gacatgcggg atcataaagg cgagcacccg cggatgggcg ccaccgacgt atgtcctttg
     3901 ataccgatag ccggcataac gatggaagag acggcggact gggcgcgtcg gctggcgtcg
     3961 cgggtaggta ccgagctgcg gctgccggtc tatctttatg aagaggccca gcccgacaag
     4021 cggcgtagca acctctcgat catccgcgcc ggcgaatatg aagggtattt caagaagata
     4081 gcgctcccgg aatggaaacc ggactttggc ccggcggaat tcgatgccag gcgcggcggg
     4141 acggtgatcg gggcgcggga ttttctcgta gcctacaatg tcaatctgaa tactacctcc
     4201 acgcgccgcg ccaatgcaat agccttcgat gtgcgggaag cgggccgtac gcttccggac
     4261 ggcacgcatc agccaggcac cctgaaatcc gtgaaagcca tcggatggtt tatcgaagaa
     4321 tatggcgtcg cgcagatctc tatgaacctg acgaatatcc gtatcacgcc ggtgcatgtt
     4381 gctttcgatg aagtcgcccg ggcggcgtcc gcgaggggta tccgtgttac gggcagcgag
     4441 ctggtaggac tcgtccccct gcaggccatg ctggatgccg gacggtattt tttagaaaaa
     4501 cagcaacgaa gcaccggggt ttccgaaaaa gaattgatcc ggatcgcgat actgtcgatg
     4561 gggctcgatg agctggcgcc tttcgttccc gaagaaagga tcatcgaata cctgctgcga
     4621 gggtcggccg accggcggct tgtcgggatg tctctgctcg atttcgccga tgaaacggcg
     4681 agtgaaagtc cggcccccgg cggtggatcg atcgccgcct atgtgggcgc attgggcgcc
     4741 gcgctcggga ctatggtggc gaacctttca tcgcataaga agggatggga cgaccgatgg
     4801 acattgtttg gcggatgggc ggagcgcggg cagggatata aggatgagct ccttcgcctt
     4861 gtagatgcgg atacccgcgc gttcaactcg atcatggcgg catttggaat gtccaaaggg
     4921 acggccgaag agaaggcctt gcggcatcaa gctatccagg ctgcgacgcg caccgccatc
     4981 gaggtgcctt tccgcgtgat ggaagtggcg gcggcgtcca tggaggtgat cagggccatg
     5041 gcgctcgatg gtaatcctaa ttccgtttcg gacgcggggg ttggcgcgct ttgcgtgcgt
     5101 gccgcagtga tgggagcttt cctgaatgtc aggatcaatg cggccggtta tgaggataag
     5161 gaattcgtga aggatatcca tgaccggggc agcaggataa tggcggaggc caataaggcg
     5221 gaaacggaaa tactgaacat cgtgcttggt aaaatgggaa gctgatatct catgatcgcc
     5281 cttcgggcga gggccgggat gccgccttag aaaggaatac cgatgttcac atagccgatg
     5341 acacgtcgcg actgatcgca atacatcgcg ctgagctcga ggggaccgag tgcgaaacgg
     5401 taggtgaatg tgagcgcata gcccgaaagg aaatcggggg tggtgaagaa actgctctta
     5461 tcgaggaagt tgttgaacag gatattggcc ttacccgtca ccataacgtt attgaacagt
     5521 tgataccgta atgccagcat cccctccgct acgctggcgg aatagaacgt gccttcgcga
     5581 agccccgcga agaccacctg gttgtggaat tgatccgtca ggcccccgat ggaaaattcg
     5641 ttcatgatat tgtggtgata gttgaggttg agcccaccct ggaggtgtac ctgtaccgta
     5701 taccggttgg taacgggaat gaattggtcg tagttggcga ggaggcgctc gtagggctct
     5761 tcggagaagc tggtatcgct gcggttgacg gtggaagcga aatactgggc ctcggggctc
     5821 tgttcgaata catggtcggc ctctacgtga aacctaaagc cgcgccgggg atagatgggt
     5881 cggtccagtg tattgctccg aacgaaaaaa tagctggtca ggaaattgtt gttgccctcg
     5941 aattccagac tcgaggtgat ggacggcgca tagtcggtgt attcccagcg gctgcccacg
     6001 ccgacggtca gatcgcggtt ggtcgaatag ccgaaacggc cgtcaagctt gaaataattt
     6061 tgatcgtaga gtcccgcctc ctggtatttg tcattgtaag tagtgatcct gaactcatcg
     6121 aattgtgtag cgagtgcgaa tgaaaaattg gctctccggc tgaagtattg caaatgttcc
     6181 gccctcgcgc ggaagttctg tccgatgttg agcgtcacca ggcttcgtga ggtcggtgta
     6241 aagaaatcgc gggtggtcag attgaggatc gcgctgatgc cgctgaattg gttgtaattg
     6301 agccccagtt tggcgaaggt aagcggcgtc tcttccactt cgaatttgat atgactgctg
     6361 ctgtcgggct gcggcgtcag cgagtaggtg atgcggctgt aataccgggt accgaaggcg
     6421 cggcggacca tcttggagag tgcggtcgcg ctataagagt gcccgctttc caggttgatg
     6481 ctctggataa aaaatgcttc gctggtatgt ttcagacctg tgacctcgta ggaagagatc
     6541 ctgacgggat agacggccgg aaggcggttt ttgacagtct gctgcggccc ataggtggcg
     6601 tccagcgaat cgaccagctt ttttagccgt ggatagagca ggcgacccgc tttcagaccg
     6661 gtctcgatga tctcgcccgc ctctccgaaa ttccccatgt tatatttgtc cagcggctgg
     6721 tagacgtaga tattacaaag cgggatctgg gtgcgggtgt cggccgcttc gcggaaaaag
     6781 gccacctgca tcaacacctg gagcggattg gtcacctggt ccggagttcg gagcggaccg
     6841 gacacattgt ctccgatgac gatatccgca cccatcttcc ggacatcgct gaccggaaaa
     6901 tttcgaacga cccctccgtc gaccagtttg tgacctccat attgaaccgc ggtaaagatc
     6961 gttgggatgg ccatgctcga tctcagcgcg gggacgatct cacccgtgtc cagcaccacg
     7021 gcctcgcctg tcgaaatatc cgtggctatg caacggaagg ggatggagaa tttcgagaaa
     7081 tccttgatat tgtaaacggg ccggaacaac tccgagaact tcatccagag ctcttctccc
     7141 tcgatgaccc cggtgttgat gcggaaccag tgattcaccc agggcagctc gacgatgtat
     7201 ttgccgtatt cgtccttctc gtccatgatc atgcttcgca tggaggattg attggacagc
     7261 agtaggtccc agtcgatgct acccgctatc ttttcgatgc tgtcggccga gtagccgacg
     7321 gcgtagagtc cgccgatgat actgcccatg ctggtgcccg tgatatagtc cactttcagc
     7381 ccggccgaat cgatggcttt gaggatgccg atatgcgcga gcccttttgc tccgccaccg
     7441 ctgaggacga gacctatttt aggacgctgg ctccggatag ccgtttggga ttgcatggcg
     7501 ggactttgcg acgcgctgct atcctgtgct gacagtcggc cgccggcaag cagaaagaag
     7561 acacagaagg agagaagggg atagtttttc aaagcgtttt ttccaattcc atgatctttt
     7621 cgaacacctg ttcatattgg cggttggcct tatccagctc gtcgcttgtc tttttatagt
     7681 cggtttccgc cttgatgaac ttattcttat ctgaatacgt ggccggatcg gccagagatg
     7741 cctcgagggc atctttctgc cgggtcagcg tggcgatctt ttgttccagg tcctggaaga
     7801 ggcgttgctg gcgctggagt tcttttttcg cctccttatt cggacgcgcg cccaaatcgg
     7861 aggggcgcgg ccggtccgac ggagggcccg gcacaggcgg tggcggggga ggagcggcga
     7921 cggcgacagg gactgcggca acggctgcct gttgtttggc agccaggttg cgttccttcc
     7981 agtctaccca ttcctcgtag gtgcccttga actcgcggat cagcccgtct tctatctccc
     8041 atatcttatt ggcagtcctg gaaatgaaat agcggtcgtg actcaccagc agatagctgc
     8101 cttcgtatct ggtcagggcc tcgatcaaca gatcgcagga atgcatgtcg aggtggttgg
     8161 taggctcgtc cagcagcagg aaattagcct tgctgatgat cgttttggcg agcgctaccc
     8221 gggccttttc accgccgctg aggacacgta tctttttttc gacgtcgtcg ccgctgaaca
     8281 ggaaacagcc gagcaggacg cgcagctcga gatcggtctt ctggctaccg caaagctgca
     8341 tttcttcgag cagcgtattg ttgacgttga gggattccag ctggtgttga gcgtaaaagc
     8401 tttcttctac attatgcccc cagacacgct ggccttcgaa gggttcggtt cccgcaacca
     8461 ttctgagaag cgtggatttc ccttttccgt tggcgccgat gagcgcgatc ttgtcgccgc
     8521 ggtctatctc cgcagacgcg ttctccacga tggtgatagg gccgaaatgt ttgctggcgc
     8581 ctttcagcga agcgagtatc
//
LOCUS       DASYSV010000147         9075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_154494, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000147 DASYSV010000000
VERSION     DASYSV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154494"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGQ51_04595"
     CDS             complement(<1..224)
                     /locus_tag="VGQ51_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035870.1"
                     /translation="MLPLRYLLRAIKIARRGAWAAITTRYTRLYLRANGVRLGHGCLS
                     NGMPELEMSVHGQFTAGCGLELQNGRHYNM"
     gene            complement(199..1437)
                     /locus_tag="VGQ51_04600"
     CDS             complement(199..1437)
                     /locus_tag="VGQ51_04600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV9035871.1"
                     /translation="MNTSLKILWLAPHPIRPGEHPAPWLVTLATELARAGHRLTVLTP
                     SPRTTQVQRIATDQGYELIIVPFKGGIRHLLSGFRTQISSMSAFLRQGASTFDVIHVH
                     GTEMQLATSVMEAQTGTPYIISIQGILSLYKDKLPQKFSKRYLYWSLASRYEKQEVRY
                     SPNFFCRTDWDQHFVRHFQPRASITTCWEMLRPDFFQYRHSFTGKGILFMGGDNPLKA
                     LTLCLKVFGHLIVRQPDLRLHIVGSVNAAAYRRLVHNIDARPLSTDNVVFHGALDAQG
                     ICRLYTECFCLYHPSLIDNSPNSVCEAQVAGLPVIATRVGGVPSLIDDGRTGLLIRKN
                     DEAGHVATLERLYRDSGLQRWISRNSREVARQRHDTATILETTLNTYRTLAAASASNV
                     PASATNVLQTTNHVTTQIPA"
     gene            complement(1434..2114)
                     /locus_tag="VGQ51_04605"
     CDS             complement(1434..2114)
                     /locus_tag="VGQ51_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV9035872.1"
                     /translation="MIKKSLQILKRNLTPPRGIDFESVYPVSDFYGDDRGTPVDRYYI
                     DRFIKDNRHRIRGHVLEVAENTYSRQFGSGIESIDVLHYEKGTPGATLIGDLSRPETL
                     PENRFDCFICTQTIHVIYNYRDAIRGARQLLKPGGILLCTLSGIAQVSRYDMDRWGDY
                     WRFTTLSAQRSFGEVFGAPNVTVDYGGNCYAAVNFLRGIALEELDRNKLGVKDPNYPI
                     LITIIASK"
     gene            complement(2125..4470)
                     /locus_tag="VGQ51_04610"
     CDS             complement(2125..4470)
                     /locus_tag="VGQ51_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEV9035873.1"
                     /translation="MADFQDLKSKAVTASLWAIVEKFSLQIVQFVVSVILARILEPKD
                     YGLIALTAIFTTLSGAIIDGGFEKTLIRAKVLQPIQVTSVFYVNLVLGCLLTVILLVT
                     AQPIALFFRAPTLAPVLRVVSLGLPINALTQVQRVLLMKELQFRKISIAQIFSALAGG
                     ITGIVMACKGMGVWALVGSLLVFQVVTLVIFWIRSDWYPSFSFSYSSIKDMLPYGSRI
                     LVVSLLFFLMQQFNTFIVGKMYSKTDLGYFNRGGRFPDLLVSLVQSIVLKLAFPLFAK
                     VRDEKAQLDELLKRTTQLVAFIVFPLLALMLVNAFDITLVIFTAKWLPSVIFMELFCF
                     ITLLEPFVVIYRELILAKGHARLLMRIFMATSIAEIALVLLLAKYGILYVVVASIISK
                     TIQYAVYLGVASGQTSISWRRQVAWIGPYIGISALMGLIVKGIGWLLSGTSMPPIAGL
                     TIKLTAGIVIYMLLARLFRLNELSFIRTLYAKVQKKIHAMHWKQDLYNAAGLAIRRSR
                     LHRLFSLKDDGRPTFPHKLVYMCGREGQRYLNASLTSVYLFWDSLPEVIVISDGTPLR
                     DIVRWPRKLEVISSMDAYRHFSDRGNPDLCRYADRIVYGKKFISLVYLAQQTPILYSD
                     TDVLWYSTPTAGGIGQGPYVKMGSDISGGYYSGSLLEGLDEHRCSENIPFNAGVMYLS
                     GDLSVYPKWSELCRRLAEDPGVGLKDFTEQTAFAILANHFNAASYFTQREILIRTDDT
                     YALQYTLKHSPGIIARHYVNTRRVAFWRDFAYICVNKKLYA"
     gene            complement(4519..5622)
                     /gene="gmd"
                     /locus_tag="VGQ51_04615"
     CDS             complement(4519..5622)
                     /gene="gmd"
                     /locus_tag="VGQ51_04615"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEV9035874.1"
                     /translation="MKTALITGITGQDGSYLAGLLLDKGYMVHGVKRRSSLINTDRID
                     HLYQDPHEKHVRFRLHYGDMTDSTNLIRLIQETQPDEIYNLAAMSHVKVSFDTPEYTA
                     NADGIGTLRLLEAIRILNLQGKTRIYQASTSELYGLVQEIPQRETTPFYPRSPYAVAK
                     LYAYWITVNYREAYGLYACNGILFNHESPQRGETFVTRKITRAVAAIALGLQDCLFMG
                     NLDAQRDWGHARDYVDAMWRILQQDTPDDYVIATGVTTPVRDFIRMAFAELGIDIEFI
                     GSGLEEKGIIAACRSEEYQLPEGTTVVAVDPAYFRPTEVDLLIGDASKARRVLNWEPR
                     YTLPMLVKEMVANDVDRLKTRMPEFTYGLMEPF"
     gene            complement(5643..6173)
                     /locus_tag="VGQ51_04620"
     CDS             complement(5643..6173)
                     /locus_tag="VGQ51_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HEV9035875.1"
                     /translation="MNTQWYAVYTKPRWEKKVAEHLAKNQFESYCPLNRVLRQWHDRK
                     KFVQEPLFSSYVFVHTEECRHIELSRISGIVNLVYWLRKPAVIKEEEIDAIRKFTAQY
                     PNVQLEKTAVNVDDKVRVIKGPLITQEGSVIAVKSRTVKIALPSLGYIMVAEVEKQNV
                     EIITPSGHTFAYEHTA"
     gene            complement(6301..8670)
                     /locus_tag="VGQ51_04625"
     CDS             complement(6301..8670)
                     /locus_tag="VGQ51_04625"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEV9035876.1"
                     /translation="MIKSNKTIREQKDSNLISFIRFRFLPYWPLFGLLVIISVAVAML
                     YLRWATPTYEAKADLLIKDANKGSGDAQTLASLNIFTTKKIVEDEIEVLQSQTLMREV
                     VRNLHLYAPVWAEGRVKAHLAYSSSPVIIEAMGFDSLYKEHSKMFLGKTAFHFDSATA
                     TIRTAAGSFPLDSWVAYPGTSLLLRFSRNPKLSETDNRAKYFMVLSPKMVTNSLIGKL
                     SVTSTNKLSSVVTLTFGDEDPHRAEDILNELITVYDHASIDDKNQLAINTLTFVENRL
                     LHVRKELDSAENRLQRYKTRNGGADLSQQGTAYLANVSSNDQKASDISMQLAVLDEVD
                     KYVRNDNGGSGIVPSTLGIQDDGLNHLLQKLYDARTNYDKMRKTIGENNPTMLSLANE
                     IQKIKPNILENIRIRKLSLQAGLANLQSTNDKYTTRLAGLPEKERTLLESSRQESILN
                     NVYSFLLQKREEASLSYASTISDSRTIDRAESSLDPISPKRTIILGIALLTALIIGGA
                     IVYTREFINTRILFRKEIETYSSVPIIAEIAAAGHSSSILIPKQSRRVVLTEQFRQLR
                     TALGLHAKRNTNRKLLVTSSISGEGKSFISANLALSLASAGKKVVLLDFDLRNPRITA
                     EFNPAAGPGLSDFLQGDIEPYEIIKSCPYDNLFICAAGGMEGDGIELSFNNRLSELFD
                     YLDGVFDFIIIDTPPVHPVSDAYVLTEYCDISLYVVRHDFTPKAFVQLLDENNKIKPL
                     KNLVIVFNGIKSRGIFKGKYGLDLGYGNEFSVKQRKTSSVVRKLLGARS"
     gene            complement(8676..>9075)
                     /locus_tag="VGQ51_04630"
     CDS             complement(8676..>9075)
                     /locus_tag="VGQ51_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEV9035877.1"
                     /translation="DRKLLMDPIVSIRMLNFKVTVLGEVGHPSVIPVPSEKISLLEAL
                     GLAGDLTINARRDNVMVIREQSGRKVIKRINLNSSELFTSPYYYLQTNDVVYVEPNKA
                     KVRSGANTAMWVSVILAALSFGIIAVTNIK"
BASE COUNT         2036 a         2334 c         2582 g         2123 t
ORIGIN      
        1 atcatattgt aatgccggcc gttctgcagt tcgagaccgc acccggcagt aaattgtcca
       61 tgcacgctca tctccagttc gggcatgccg ttgctaagac agccgtgtcc cagacggacc
      121 ccgttggcgc gcagatatag ccgtgtgtac cgggtcgtaa tggccgccca cgcaccgcgt
      181 cgggcgatct tgatggctct aagcaggtat ctgagtggta acatgattgg tggtttgaag
      241 gacattcgtt gcggacgccg gcacgttgga agccgaagcg gcagcgaggg tccggtaggt
      301 attgagggtt gtttccaaaa tggtcgccgt atcgtgccgc tgccgggcga cctcgcgact
      361 gttgcggctg atccatcgtt ggagaccgga atccctgtag agtctttcca gtgtggccac
      421 atgacccgcc tcgtcattct tacggatcag caaacccgtc cgtccgtcat cgatcagcga
      481 aggcacgccg ccgaccctcg tcgctatcac cggcaacccg gccacctgcg cctcacaaac
      541 tgagttagga ctattatcga tcagcgaagg atggtagaga caaaaacact cggtatacag
      601 cctgcatatg ccctgtgcat ccagtgcgcc atggaatacc acattgtccg tgctcagcgg
      661 acgggcgtcg atattgtgta ccagacggcg gtaggcagcc gcgttgacgg agccgacgat
      721 atggagccgc agatcgggtt gacggacgat caggtgtccg aagaccttca gacacagcgt
      781 cagcgccttt agcggattgt cgccgcccat gaaaaggata ccttttcccg tgaatgaatg
      841 ccggtattga aaaaaatctg gacgaagcat ttcccagcaa gtcgtgatcg aggcgcgtgg
      901 ctgaaaatgc cggacaaaat gctggtccca gtccgtgcgg caaaagaaat tgggcgaata
      961 gcgaacttcc tgtttttcat accggctggc caacgaccag tataggtatc gtttggagaa
     1021 tttctgcggc aatttgtctt tatatagcga aaggatgccc tgtatggaaa tgatataggg
     1081 cgtccccgtt tgtgcttcca ttaccgaagt ggcgagctgc atttccgttc cgtgtacatg
     1141 aatgacatcg aacgtactgg ccccttgccg caaaaaggcg ctcatggacg atatctgtgt
     1201 ccggaagccg ctcaacaggt ggcggatgcc gcctttgaag gggacgatga tgagctcata
     1261 gccctggtcg gttgcaatgc gttggacctg cgttgttctt gggctgggtg tcagcacggt
     1321 gagtcgatga ccggcgcggg ccagctcggt agccagggtg acgagccagg gagcggggtg
     1381 ttcgccgggc ctgatggggt gcggcgcgag ccagaggatt ttcagggacg tattcatttt
     1441 gaagcgatga tggtgataag aatgggataa ttgggatctt ttactcccag tttgttacgg
     1501 tcaagctctt ccagagcgat accgcgcagg aaattgacgg cagcgtagca gttaccgccg
     1561 tagtcgacgg tgacattggg cgcaccgaat acttcgccga acgagcgttg ggcggacagg
     1621 gtggtgaaac gccagtagtc accccagcgg tccatgtcgt agcgcgacac ctgtgcgata
     1681 cccgacaagg tgcacaacag gataccaccg ggtttcaaca actgacgggc gccccggatg
     1741 gcatcccggt aattgtaaat gacatggatg gtctgggtgc agatgaaaca atcgaaccgg
     1801 ttttcgggaa gcgtttcggg ccggctcaga tcgccgatca gcgtcgcgcc cggcgtgccc
     1861 ttttcataat gcagcacgtc tatcgactcg ataccgctgc cgaattgccg ggagtaggta
     1921 ttttccgcta cttccaggac atgtccccgg atacgatgac gattatcctt gatgaacctg
     1981 tcgatataat accggtcgac cggcgtgccc ctgtcgtccc cgtaaaaatc agagactggg
     2041 tacaccgatt cgaaatcgat gcccctcggc ggggtcagat tccttttcag tatttggaga
     2101 gatttcttga tcatagtcat tgtttcacgc gtaaagtttt ttattgacgc agatgtaggc
     2161 gaagtcccgc cagaaagcga cccgcctggt gttgacataa tgtcgtgcga tgatgccggg
     2221 agaatgtttg agggtgtact gaagagcata ggtatcatcc gtacggatga ggatctcgcg
     2281 ttgagtgaaa taggaggctg cgttgaaatg attggccaag atggcgaatg ccgtttgttc
     2341 ggtgaaatcc ttcagaccca cacccggatc ttcggccagc cgtcgacaga gctcggacca
     2401 tttgggatag accgaaaggt ctccgctgag atacatgacc cctgcattga agggtatgtt
     2461 ttcggagcaa cgatgttcgt cgagaccttc cagcagcgag cctgaataat agccgccgct
     2521 tatgtcgctg cccattttta cataaggacc ctgcccgatg ccgccggccg tgggcgtcga
     2581 ataccagagc acgtcggtat ccgaataaag gataggcgtc tgttgcgcca ggtagacgag
     2641 tgatatgaat ttcttaccgt aaacgatccg gtcggcgtag cggcataggt ccggattgcc
     2701 tctgtcggaa aaatgccggt aagcgtccat cgatgaaatg acttccagct tacgcggcca
     2761 tcgtacgatg tcccggagcg gcgttccatc ggagatgacg atcacctccg ggagactgtc
     2821 ccagaaaaga tatactgaag tcagcgaggc gttgagatag cgttgtcctt ctcttccgca
     2881 catatagacc agcttgtgag ggaaggtagg tctgccgtcg tcctttagag aaaagagacg
     2941 gtgcagcctt gacctgcgga tagcgagacc cgcggcatta taaaggtcct gtttccaatg
     3001 catggcgtgg attttttttt ggaccttggc atatagggtc cggataaaag agagttcatt
     3061 gagacggaat agacgtgcga gcagcatata aatgacgata ccggccgtca gcttgatggt
     3121 cagccccgcg atgggcggca tggacgttcc cgacaggagc cagccgatac ccttaacgat
     3181 cagacccatc agcgcggaga taccgatata gggaccgatc caggccacct ggcgtcgcca
     3241 ggaaatggac gtttgtcccg acgctacccc cagatagacg gcgtattgga tggtcttgct
     3301 gatgatagag gcgacgacga catacaggat gccgtatttc gcaagcagca ggacaagtgc
     3361 tatttcggcg atggacgtag ccatgaagat gcgcatcagc agacgggcat gacctttcgc
     3421 caggatcagc tcgcgataga tgactacaaa cggctccagg agggtgatga aacaaaacag
     3481 ctccataaag atcaccgagg gaagccactt ggccgtaaag atcaccagcg tgatatcgaa
     3541 ggcgttgacc agcatcagcg ccagcagcgg aaagacgatg aaggccacca gctgcgtggt
     3601 ccgtttgagc agctcgtcca gttgcgcctt ctcatccctg accttggcaa acagcgggaa
     3661 agccagttta agcacgatgc tttgcacgag actgacgagc agatcgggaa atcgcccgcc
     3721 tcggttgaaa tagcccagat cggtcttgct gtacatcttg ccgactatga aagtgttgaa
     3781 ctgctgcatg agaaagaaca gcaggctcac gacgagtatc ctgctcccgt acggaagcat
     3841 gtctttgatg gacgagtagg aaaagctgaa gctgggatac cagtcgctgc gtatccagaa
     3901 gatgacgagg gtcaccacct ggaaaacgag cagtgatccc accagcgccc atacgcccat
     3961 gcctttgcag gccatgacga taccggtgat gcctccggcc agcgcgctga agatctgcgc
     4021 aatcgatatt ttccggaact gaagctcctt catcagcaat acgcgttgca cctgggtgag
     4081 cgcattgatc gggagaccca gcgacaccac ccgtaatacg ggagcaagcg tcggtgcccg
     4141 gaagaagagc gctatcggct gcgcggttac caggaggatc accgtcagca gacaacccag
     4201 aacgagattt acgtaaaaaa cactggtgac ctgtatcggc tgaaggacct tggccctgat
     4261 cagtgttttt tcgaaaccgc cgtcgatgat agcgcccgac agcgtggtga agatggccgt
     4321 gagggcgatc agtccatagt ccttcggctc gagtatcctg gccaggataa cgcttaccac
     4381 gaactgaacg atctgtagcg agaatttctc gacgatcgcc cacaacgagg cggtgactgc
     4441 ttttgatttc aggtcctgaa agtctgccat tctaaatgcg gaaaaaacga gccgccggag
     4501 gcggctcgga gtaattcatc aaaaaggttc catcagcccg taggtgaatt cgggcattcg
     4561 ggtcttcagc cggtcgacat cgttggcgac catttcctta accagcatcg ggagggtata
     4621 gcggggctcc cagttcagca cgcgtcgtgc cttggaggca tctccgatga ggaggtctac
     4681 ttcggtgggc cggaaatagg ccggatcgac ggcgaccacc gtcgtgcctt ccggtagctg
     4741 gtattcttcc gatcggcatg cggcgatgat gcctttttct tcgagaccgc tgccgatgaa
     4801 ttcgatgtcg atacccagct cggcgaaagc catgcggatg aaatcgcgta ccggggttgt
     4861 gacacccgtg gcgatgacat aatcgtcggg agtgtcctgc tgcaggatgc gccacatggc
     4921 atccacgtaa tcgcgtgcat gaccccagtc gcgttgagcg tccaggttgc ccatgaaaag
     4981 acagtcctgc aaacccaggg cgatggcggc caccgccctg gtaattttgc gagtgacgaa
     5041 ggtttccccc ctttgggggc tttcgtggtt gaacaggatc ccgttgcagg cataaaggcc
     5101 ataggcctca cgatagttga cggtgatcca gtaagcgtac agcttggcga cggcgtaggg
     5161 actccgggga tagaaagggg tggtttcccg ctggggaatt tcctgtacca gcccgtataa
     5221 ttcggatgta gaagcctggt aaatgcgagt tttcccttgc aggttaagaa ttcggatagc
     5281 ttcaaggaga cggagggtgc cgataccatc ggcattggcg gtgtattcag gcgtatcgaa
     5341 gctcactttt acatggctca tcgccgccag attatagatc tcatccggtt gcgtctcctg
     5401 gatcaggcgg atcaggttgg tgctgtccgt catgtctcca tagtgaagac gaaaccgtac
     5461 gtgtttttca tgcgggtcct gatagaggtg gtcgatgcga tcggtattga tcagggacga
     5521 ccttcgtttg actccatgga ccatatagcc tttgtccaat agtaacccgg ccaggtagga
     5581 tccgtcctga ccggtgattc cagtaattaa cgctgtcttc atgatttgat tttaagaggt
     5641 ttttatgcgg tgtgttcgta agcgaaagtg tggcccgagg gagtgatgat ctcgacattc
     5701 tgtttttcca cctcggccac catgatatat cccagcgatg ggagagcgat ctttaccgtc
     5761 ctgctcttca ctgcgatgac gctgccttcc tgtgtgatga gcggtccctt gatgaccctt
     5821 accttatcgt ctacattgac agctgtcttt tcaagctgca cgttcgggta ttgagcggtg
     5881 aacttcctta tcgcatcgat ctcctcctct ttgatgacag ctggtttacg cagccagtag
     5941 accaaattga cgatgcccga tatgcggctt agttcgatat gacggcattc ttccgtatgg
     6001 acgaatacat aagaggagaa taaaggctcc tgaacgaact tcttccggtc atgccattga
     6061 cgaagcaccc tgttcagcgg acaataactt tcaaactggt tctttgccaa atgctctgct
     6121 actttctttt cccaccttgg cttagtgtaa acggcatacc attgagtatt catagttata
     6181 ctttttttga gatgattgtg aatttgtgaa aaggctccgc ccatctcagt aacagggcct
     6241 gtttccgagc gacgatgaag tggtaggtta agaaaatccg cctcaagcgg ctgaagtcga
     6301 tcaggatctg gcgccgagca atttccggac gacggaggag gtcttcctct gtttaaccga
     6361 aaactcgtta ccatatccga gatcgagacc atatttccct ttgaagatgc cccgcgattt
     6421 gatgccattg aagacaatga cgaggttctt caggggtttg atcttattgt tctcatccag
     6481 caactggaca aaagccttgg gtgtaaaatc gtgccggacc acatacagcg agatatcgca
     6541 gtattccgtc aacacgtatg catcgctgac cggatgcacc ggcggggtgt cgatgatgat
     6601 gaaatcgaac acaccgtcca ggtaatcgaa cagctcgctc agccggttgt taaaagacag
     6661 ctcgatcccg tcgccttcca tcccgccggc cgcgcagatg aacaggttgt cgtagggaca
     6721 actcttgatg atctcatagg gttcgatatc cccctgcagg aaatcggaca gacccggtcc
     6781 tgccgccggg ttgaactcgg ccgttatccg cggattacgc agatcgaaat cgagcaggac
     6841 cacctttttc cctgcggagg ccagtgaaag cgccaggttg gcgctgataa agcttttacc
     6901 ttcgccggaa atgctggaag tcaccagcag cttgcggttg gtatttcttt ttgcatgcaa
     6961 tcccagcgcc gtgcgcaatt gccggaattg ttcggtgagg acgacgcggc ggctctgttt
     7021 agggatcagg atggaggacg aatgtcccgc agccgcaatc tcggcaatga tcggcacgga
     7081 gctataggtc tcgatctcct tgcggaaaag gatcctggta ttgatgaatt cccgggtata
     7141 tacgatggcg ccgccgatga tcagcgcggt caaaagcgcg atgcccagta tgatcgttct
     7201 tttcggtgaa atgggatcca gcgacgattc tgccctgtcg atggtcctgc tgtcggatat
     7261 cgtcgatgcg taggataacg aggcttcttc ccgtttttgc agcaggaagg agtagacgtt
     7321 gttgagaatg gattcctgcc ggctgctttc gagcagggtc ctttcctttt ccggcagccc
     7381 ggccagacgc gtggtatatt tgtcgttggt actttgcaga ttcgccagcc ccgcctggag
     7441 gctcagcttc cgtatccgga tattctccag gatgttgggc ttgatctttt ggatctcatt
     7501 ggccagcgac agcatcgtgg ggttgttttc gccgatggtc ttgcgcatct tgtcgtaatt
     7561 cgtcctggcg tcatacagct tctggagcag gtgattcagc ccgtcatcct ggatgccgag
     7621 ggtagagggg acgatccccg aaccgccatt atcgttccgc acgtatttgt ctacttcgtc
     7681 aagcaccgcc agctgcatgg aaatatcgga cgccttctgg tcattgctgc tgacgtttgc
     7741 cagataagcc gtgccctgct ggctgagatc ggcgccgccg ttgcgggtct tgtaacgctg
     7801 taaccggttc tccgccgaat ccagctcctt acgaacatgc agcaaccggt tctcgacaaa
     7861 agtgagggta ttgatcgcta attgattctt gtcatcgatg gatgcatggt catacaccgt
     7921 tatcagctcg ttgagaatat cttccgcccg gtgcgggtcc tcatcgccaa aagtcaaagt
     7981 gacgacggaa gacagcttat tggtcgaggt cacgctcaat tttccgataa ggctgttcgt
     8041 gaccattttg ggactcaaga ccatgaagta tttagcccgg ttgtcggttt ccgataactt
     8101 cggattgcgg ctgaaccgaa gcaaaagcga ggttccggga taagccaccc agctgtccag
     8161 cgggaacgac ccggccgccg tccggatggt agcggtagca ctgtcaaaat ggaacgcggt
     8221 cttgcccagg aacatcttgc tgtgttcttt gtacaaactg tcgaatccca tggcctcgat
     8281 gatgacgggg gaggagctgt atgccagatg cgctttgacc cggccttcgg cccacaccgg
     8341 cgcatagaga tggagattac gtaccacttc ccgcatcagg gtctgcgact gaagcacttc
     8401 gatctcgtcc tccacgatct tttttgtggt aaaaatattc agcgaggcca gcgtctgcgc
     8461 gtcgcccgat cccttgttgg catccttgat cagcagatcg gcctttgcct cataggtagg
     8521 agtggcccac cgaaggtata acatggctac ggcgacggaa atgatcacga gcagaccgaa
     8581 cagcggccag taaggcagga aacggaacct gatgaaactg atcaggttgc tgtctttctg
     8641 ttcgcggatc gttttattcg actttatcat gctcatcatt ttatgttggt aactgcgatg
     8701 atcccgaagg aaagggcggc cagtattacg gacacccaca tggcggtgtt agcgccgctg
     8761 cggacctttg ccttattggg ttcaacatat accacgtcat ttgtctgaag atagtaatag
     8821 ggtgaggtga acagttcgga ggaattgaga tttattcgtt tgatgacctt ccggcccgat
     8881 tgttcgcgga tgaccatcac attgtcacgc cgtgcattga tggtgaggtc gcctgcaagg
     8941 ccaagggctt ccagcagtga gatcttttca ctgggtacgg ggatcaccga aggatgcccg
     9001 acctcgccga gaacggtcac cttgaaattc agcatccgga tactgacgat gggatccatc
     9061 agcagtttcc ggtca
//
LOCUS       DASYSV010000148         4114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_154596, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000148 DASYSV010000000
VERSION     DASYSV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154596"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VGQ51_04635"
     CDS             complement(<1..221)
                     /locus_tag="VGQ51_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV9035878.1"
                     /translation="MIREILGVAVLLQGLGVMGQQPALDMDSIAAEGMPRLVSRQFSF
                     TEGASVDRSGNVFFTDQPNDKIWEYTVDG"
     gene            266..3352
                     /locus_tag="VGQ51_04640"
     CDS             266..3352
                     /locus_tag="VGQ51_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9035879.1"
                     /translation="MNTRRTIFGCLLTTQLLPTLLIAQQIPHFIEKLTTSAGLSSNTI
                     TDITQDDQGFLWIATTDGLNRFDGTEITQFYHQDDSHSLPHNFIYCIKRLPGNYLAIG
                     TEGGLGFYDGHTGLFQNFYYRSDSAMAPFNNSILNMETDADGNLWAISKNCIFVFDPL
                     RRLKKLIPSGFTHTLATRNRINFVEKIWPLTDGDMLLYLHDGWRIYSKKTGLLTDSAS
                     SPRLKQLTFLHLLDSTPSHPAGYISSRLFKVFDKFFLLLHRDSLFLFDETGRRRNACH
                     FPYDRYPYVSWSQRIAPIDSTRILLLFHNYGLAILSIDWRNKTPVLDSLSTPLLCDQK
                     YNTVLCDRQNNWWLATSRVGLQKIVPSRQCFTGVTLLDNSSHRTIKYEANSFSRYGRT
                     LWVATYGDGFFAIDPSTHHQQQHRLLNTGIPTWTDFIWNVRQIDKDTLWVGTQVGMFW
                     YCLTTRTNGRLPPFPGKPPALDEVSVTTQFRDSHGLVWMGLGKGNGLCCFDSARHRFD
                     YYPGNSEKGYPLRYPLSIAEDTKGDLWMTGDASNSLVKWHRDSGLFTVIPLPSAVRIH
                     IGPLNGIVIDHDSVFWLSSLTRGLVRFVPSTGSLSVYGHENGLANSYTGSLFKDSTGR
                     IWMMTEGGLACFNPRTESFLNYTEDNGLPVTCLTAEFFYDPLTGRLYNGGHGGFFYFL
                     PGGIDPYGRPPRPMITSISVNGNQRIPDPDSTAEFHSQENDISIRYAAVDLTDGHTTR
                     YAWRLLGADTAWVMADRQRQINFSRLAPGKYTFLVRAATGNGVWNPVPAAFSFRIYPP
                     FTQTPGFYTLLFLAIASLAWLLYRYRRRQTQRSRQIRSEISRNLHDEVGANLTNISLS
                     SLLARRQLHNDEAVSQLLERIYQDSQQVSESMRDIVWSIDPNIETLGEALPRMLHYAS
                     GLLEAKDIGLEAAVAPEVEQLKLNMHQRRDIYLIFKEAVNNMARHSKASSARIRFDLD
                     GHALQMTVQDNGSGFDTLAHAGHNGLRNMQERASRHQWKLNIRSSPLHGTTITLKT"
     gene            3426..4058
                     /locus_tag="VGQ51_04645"
     CDS             3426..4058
                     /locus_tag="VGQ51_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9035880.1"
                     /translation="MTTRIAIFEDNARLRQSLKVLLEGVEGYTVCGDYENCDHAIDVM
                     NQHHPDLVIMDIDMPGVDGIEGLRIIKEQYPRTHIIMHTVLEDEERLFSCLCNGANGY
                     ILKNTSFVHLLEAIGNVLNGGAPLSPPIAKKVLESFRHSDASRLKYHLSDREKEVLAY
                     LVKGYSYKMIAGTCHISLDTVRGHIRNIYSKLHVNCGREAVSKALRDRIV"
BASE COUNT          952 a         1395 c          890 g          877 t
ORIGIN      
        1 ttcccatcca cggtatattc ccagatcttg tcattgggct gatccgtaaa gaacacattc
       61 ccgctgcggt ctaccgacgc cccctcggta aagctgaact gacgcgacac caaccgcggc
      121 attccttccg ccgcgatcga gtccatatcc aatgccggtt gctgtcccat tacgcccaat
      181 ccttgcaaca aaacggccac gcccaatatt tccctgatca tattattttt ttagcaatat
      241 aattcatatc ggtaacttgc catacatgaa caccaggcgc acgatattcg gttgcctgct
      301 aaccacacag ctgctcccga ccctcctcat cgcgcagcag attcctcatt tcatagaaaa
      361 attaaccacc tccgcaggtc tcagcagcaa taccattacc gatataactc aagacgacca
      421 gggcttcctc tggatcgcga cgaccgatgg cctcaaccgc ttcgacggta ccgagatcac
      481 ccaattctac caccaggacg acagccattc cctgccgcat aattttatct attgtatcaa
      541 acgcctgccc ggcaactatc tcgccatcgg taccgaaggc ggcctcggct tctatgatgg
      601 ccacaccggc ctttttcaaa atttctacta ccgatccgat agcgcgatgg cccctttcaa
      661 taattccatc cttaatatgg agacggacgc cgacggcaat ctctgggcca tttcaaagaa
      721 ttgcatcttc gtcttcgatc cgctaagacg attgaaaaag ctcattccct ccggcttcac
      781 acataccctc gctacccgca acaggatcaa ttttgtagag aagatatggc ccctgaccga
      841 cggcgacatg ttgctatatc tgcacgacgg ttggcgaatt tactcgaaaa agaccggcct
      901 cctcaccgat tcagcctctt caccccgcct caaacagctg accttcctgc acctgctgga
      961 ttcgacacct tcccatccgg ccggctacat ttccagccgc ctcttcaagg tgttcgacaa
     1021 attcttcctg ctactccacc gcgatagcct cttcttgttc gatgaaaccg gccgccgtcg
     1081 caacgcatgc cacttcccgt atgaccgcta tccctacgtc agctggtccc aacgcatcgc
     1141 ccccatcgac tccacaagga tactgctcct gtttcataac tatggtctgg ccatcctttc
     1201 catcgattgg cggaacaaaa cacccgtgct ggattcgctg tccacccctt tgctctgcga
     1261 ccagaaatac aacaccgtct tatgcgaccg tcagaacaac tggtggctcg cgaccagccg
     1321 cgtcggtttg caaaagatcg ttccctcccg ccaatgcttc accggtgtca ccctcctcga
     1381 caactcatcc caccggacca taaaatacga agcgaattcc ttcagccgat atggccggac
     1441 actttgggtc gctacctatg gcgatggctt cttcgccatc gatccctcca cccaccatca
     1501 gcaacaacac cgcctgctga acaccggcat acccacctgg acggatttca tctggaacgt
     1561 ccgccagatc gataaggata ccctatgggt aggcacacag gtaggtatgt tctggtattg
     1621 cctcaccacc cgtaccaacg gtcggttgcc tccctttcca ggtaagccgc cagcactcga
     1681 cgaagtctcc gttacaaccc agttcaggga cagccatggc ctcgtatgga tgggcctggg
     1741 aaaaggcaac ggcctctgct gtttcgatag cgcacgacac cgcttcgact actatccggg
     1801 caatagcgaa aagggctacc ccctgcgata tcccctttcc atcgccgagg atacgaaggg
     1861 cgatctctgg atgaccggcg atgccagcaa tagcctcgta aaatggcatc gcgattccgg
     1921 cctttttacg gtgatccccc ttccatccgc ggtccggata cacatcggtc cgctcaacgg
     1981 catcgtcatc gatcacgatt ccgtattctg gctttcctcc ctaacccgcg ggctggtgcg
     2041 tttcgttccc tccaccggct cgctgtcggt atatggacat gagaatgggc tggccaatag
     2101 ctacaccggc agcctcttta aagattcgac aggccggata tggatgatga cggaaggagg
     2161 tcttgcctgc ttcaacccgc ggaccgaatc ttttcttaac tatacagagg acaacggtct
     2221 gcccgtcacc tgcctgacgg ccgaattctt ttacgatccg cttaccggac ggctgtacaa
     2281 cggcggtcac ggcggcttct tctatttcct ccccggcggc atcgacccat acggaagacc
     2341 gccccgccca atgatcactt ccatttcggt caacggaaac cagcgcatac ccgatcccga
     2401 tagcaccgcc gaatttcatt cgcaggagaa cgatatatcc atacgctatg ctgccgtcga
     2461 tctcaccgac ggacacacca cccgctacgc ctggcgactg ctgggtgccg acacggcatg
     2521 ggtgatggcc gaccgccaga gacagatcaa tttcagccgc ctcgccccgg gtaaatatac
     2581 ttttctcgtg cgtgcggcta ccggcaacgg cgtttggaac ccggtcccgg ccgcattcag
     2641 cttccgcatc tatccgcctt tcacgcagac ccccggattc tataccctcc ttttcctggc
     2701 tatcgcttcc cttgcctggc tcctgtatcg ctaccgccgc cggcagaccc agcgctccag
     2761 acagatccgc agcgagatat cccgcaacct tcacgatgaa gtgggtgcca acctcaccaa
     2821 tatcagcctg agcagcctcc tggccagacg acaattgcac aacgatgaag ccgtcagcca
     2881 gctgctcgaa cggatctatc aggacagtca acaggtctcc gaatccatgc gggacatcgt
     2941 ctggagcatc gatcccaata tcgaaaccct cggcgaggcc cttccccgaa tgctgcatta
     3001 tgcctccggt ctcctcgagg caaaagacat cgggctcgaa gcggcggtcg ctcccgaagt
     3061 cgagcagctg aaactaaaca tgcaccaacg acgcgacatc tatctcatct ttaaagaggc
     3121 agtgaacaat atggccagac attcaaaagc ctccagcgcc cgtatccgct tcgatctcga
     3181 cggacatgcc cttcagatga ccgtccagga caatggttcc ggcttcgata cgctggccca
     3241 cgcaggacac aacgggctca ggaacatgca ggaaagagcc agccgtcacc aatggaagtt
     3301 gaatatccgt tccagccccc tccacggcac taccatcacc ctcaagacat aaacatgcta
     3361 ttgacaaccc accccctgat cttaacttta ccaaaggatc ccgcgccacc ggcacccaat
     3421 ctgccatgac tacccgcatc gccatattcg aagataatgc ccggcttcgc cagtcgctaa
     3481 aagtactgct cgaaggcgtc gaaggctata cagtatgcgg agactatgaa aattgcgacc
     3541 acgccatcga tgtgatgaac cagcaccacc ccgatctcgt tatcatggat atcgatatgc
     3601 ccggcgtgga cggcatcgaa ggactccgca tcatcaagga acaatacccg cgcacccata
     3661 ttatcatgca caccgtcctg gaggacgaag aacgcctgtt cagctgtcta tgcaacggag
     3721 ccaacggcta tatcctcaaa aatacttcct tcgtccacct cctcgaagcc atcgggaatg
     3781 tactcaacgg cggagccccg ctctcccctc ccatcgccaa aaaagtgctg gaatcattcc
     3841 gccactccga tgccagccgg ctgaaatacc atttgtccga ccgggaaaag gaagtgctcg
     3901 cctatctcgt caaaggctat agctataaga tgatcgccgg cacctgccat atcagtctcg
     3961 acaccgtccg cggccacatc cgcaacatct actccaagct ccatgtcaat tgcggccgcg
     4021 aagccgtctc aaaagccctc cgcgaccgca tcgtctgatc tcaccccctc tcctccgcga
     4081 ccgcatcgtc tgatctcacc ccctctcctc cccc
//
LOCUS       DASYSV010000149         4795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_412539, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000149 DASYSV010000000
VERSION     DASYSV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412539"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VGQ51_04650"
     CDS             complement(<1..619)
                     /locus_tag="VGQ51_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035881.1"
                     /translation="MSYARSYVQCAHCIMDTNDDPDITFNAEGVCNHCQTYKYEEEHY
                     VFSGEEGQRRFDDYIRQMKEDGKNKPYDCIIGISGGVDSSYLALVAKENGLRALGVHC
                     DNGWNSELAVNNIESIVKTLDMDLYTYVIDWEEFRDIQLSYFKANVIDIEAITDLAFR
                     AVLEKTSIKYNQKYFLSGDNVVTESVLPRAWICKDYQNLINIHKRY"
     gene            complement(638..1654)
                     /locus_tag="VGQ51_04655"
     CDS             complement(638..1654)
                     /locus_tag="VGQ51_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9035882.1"
                     /translation="MSGLKFGIIGCGRIAQRHAEHIQKTGQLIAVCDIEEKKAAELAA
                     KYGVKSYKLVKDMLSAHPEMEVVAVCSPNGLHATHTIQALKAGFHVLCEKPMALSVQD
                     CGDMINTAEKSNKRLFVVKQNRFNPPVVAVKQLIEEGRLGKIFSVQLSCFWNRNEEYY
                     RNSWKGTKDLDGGTLFTQFSHFIDLLYWIVGDVKEAQALIGNFHHQGAIEFEDTGCVS
                     LKFYNGAIGTINYTVNSFGGNMEGSLTIFGEKGTVKIGGQYLNELEYQKIDGYEIKDL
                     PPGNPPNNYGQYLGSMSNHDKVYQNVVEVLTNGGVIATNGFEGLKTIEIIDKIYSSAP
                     KQQF"
     gene            complement(1651..2400)
                     /locus_tag="VGQ51_04660"
     CDS             complement(1651..2400)
                     /locus_tag="VGQ51_04660"
                     /inference="COORDINATES: protein motif:HMM:NF038364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HEV9035883.1"
                     /translation="MSLVRIIPVLLLKEGGLYKTVKFKEPKYVGDPINTVKIFNEKEA
                     DELVLLDYTATREKRDPDFKKIAEIAGEAFMPMTYGGGIRTLENAKKVFDSGFEKIVL
                     NSVLYNDLSIIEGIANIYGAQAVVGSIDVGRNLFGKQKAYSVSGTKNSGLYPVGWATT
                     LEKAGVGEIFLNAIDRDGTWEGYDIPLIRAVSAAVKIPVVACGGASGMADIERAVREG
                     GASAAAAGSLFVYQRKGMGVLVSFPTQKLKI"
     gene            complement(2407..3018)
                     /gene="hisH"
                     /locus_tag="VGQ51_04665"
     CDS             complement(2407..3018)
                     /gene="hisH"
                     /locus_tag="VGQ51_04665"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEV9035884.1"
                     /translation="MTIIVNYGLGNLTSIKNMCKRIGVEADISGDPARIEEADKLILP
                     GVGHFKKGMENLNSGGTRQLLDRLVLAERRPILGICLGAQLMTGHSEEGDVDGLGWVD
                     AVTVRFEEAAMNGLKIPNMGWREINRCGESPLWADLPAESRFYFVHSYHFRFTQPDEV
                     TATAVYGYEYACAFRKDNIFGTQFHPEKSHKFGMKVLENFFKG"
     gene            complement(3015..3665)
                     /locus_tag="VGQ51_04670"
     CDS             complement(3015..3665)
                     /locus_tag="VGQ51_04670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV9035885.1"
                     /translation="MLQKIAYFLKRPLQLPIVLLTKTIPYFRWIYRTRDYQNYVNFEF
                     WFTQKILNWGGNRKAYWPVHFTSKVYDVENILVGIDAYPGTMGGCYITGRGGLTIGDY
                     TQIAPNVVIVTANHDLYDSRKHICKPVKIGRYCWIAAGAKIMPGVELGDWTIVAAGAV
                     VTTSFPDGYAVLGGVPAKKIKDLEKEKCVEFRCEHEYHGYIPASKFKKFREKNLKI"
     gene            complement(3637..4512)
                     /locus_tag="VGQ51_04675"
     CDS             complement(3637..4512)
                     /locus_tag="VGQ51_04675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9035886.1"
                     /translation="MAANSGMTPLVSVLMTAYNREKYIGSAMESVLNSGYKNLELIIV
                     DDGSKDNTVEIARGYADKDPRVRVYINEKNLGDYPNRNQAAAYANGKYLKYVDADDYI
                     YPWGLGLLVDMMEAFPSAGWGLCSLEQYVPKPFPFQLSPKEAYEFHYLGPGLFHKAPL
                     SSIIRTDAFRSVGGFAPIRMAGDFEMWHRLGQKYPVVLMPHGIVWYREHDAQEMNSHR
                     KFIRTYVDITLKYFSDASCPLDKPTVQKILRAGRRSIYKEVLKGALTLNTHRIADNWI
                     RLNQYRHASKDRLFS"
     gene            complement(4491..>4795)
                     /locus_tag="VGQ51_04680"
     CDS             complement(4491..>4795)
                     /locus_tag="VGQ51_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035887.1"
                     /translation="TGEQKRSFTHVEDICRQIFRVVQDPQSKNNTFNIAGETYSLKEV
                     ADAIGRKFSVPTVLTPWPAIDEKLESGDTIFDSAKLENLMRNPLQNSFTTWLQTVA"
BASE COUNT         1010 a         1312 c         1190 g         1283 t
ORIGIN      
        1 cgtaccgttt gtgaatattg atcagatttt gatagtcctt gcatatccag gcccttggca
       61 aaacggattc ggtcacgaca ttatccccgg agaggaaata tttttgattg tatttgatgg
      121 atgtcttttc gaggaccgcc cggaatgcca ggtcggtaat ggcttcgata tcgatcacgt
      181 tcgctttgaa ataggacagc tggatatccc ggaactcttc ccagtcgatg acataggtat
      241 aaagatccat atcgagtgtc ttgacgatgg actcgatgtt gttgaccgcc agctcggaat
      301 tccatccgtt gtcgcaatga acgccgaggg cgcgaagacc gttctctttg gcgacgaggg
      361 ccagataaga gctgtccact ccaccgctga taccgatgat gcagtcataa ggcttgttct
      421 tcccgtcttc cttcatttgc cggatataat cgtcgaatct ccgctgtcct tcttccccgc
      481 tgaatacgta atgttcttcc tcgtatttat atgtttggca atgattgcaa acgccttccg
      541 cattgaaggt gatatcgggg tcatcattgg tgtccatgat acaatgggca cattgcacat
      601 aacttctcgc gtagctcatt ttcgtcagtt cgatgtttta aaattgctgc ttaggcgcgc
      661 tgctatatat cttgtcgatg atctcgatcg ttttcagtcc ctcgaatccg ttggttgcga
      721 tcacaccgcc attcgtcaat acttcgacga cattctgata gaccttgtca tgattgctca
      781 tcgagccgag atactgtcca taattgttcg gcggattgcc ggggggaagg tctttgatct
      841 catacccgtc gatcttctgg tattccagct cgttgaggta ttgaccgccg atcttgaccg
      901 ttcctttttc gccgaaaatg gtcagcgacc cttccatatt cccgccgaag ctgttcaccg
      961 tataattgat cgtcccgatc gcgccgttat agaatttgag gctgacgcag ccggtgtctt
     1021 cgaattcgat ggcgccctgg tgatggaagt tgccgatcag cgcctgcgcc tccttgacat
     1081 ccccgacgat ccaatagagc agatcgataa aatgactgaa ctgggtaaat agggtacctc
     1141 catcgaggtc tttcgttcct ttccaggaat tgcggtagta ttcctcgttg cgattccaga
     1201 aacagctgag ctggacgcta aagatcttcc ccagtcgtcc ttcttcgatc agctgtttga
     1261 cagccaccac cggtgggttg aaccggtttt gcttgacgac gaagagccgc ttgttggact
     1321 tttccgccgt attgatcatg tcaccgcagt cctgtacgct aagggccatg ggtttttcgc
     1381 aaaggacgtg aaaccctgct ttcaacgcct ggatcgtgtg cgtggcatgc agaccgttgg
     1441 gtgagcagac ggccaccact tccatttcgg gatgcgccga aagcatgtct ttcaccaatt
     1501 tgtacgattt aaccccatat tttgccgcga gttccgcggc ctttttctcc tctatatcgc
     1561 aaaccgcgat cagttgtccc gtcttttgaa tgtgctccgc atgccgttgg gcgatacggc
     1621 cacaaccgat aattccgaat tttaatccac tcatatcttt agtttttggg tagggaaact
     1681 gaccagtacc cccataccct ttcgctgata aacgaatagg ctgccggctg ctgctgccga
     1741 tgcgcctcct tcccggaccg cccgttcgat atccgccatt cccgatgccc cgccgcaggc
     1801 aaccaccggg atctttaccg ccgcgctgac tgccctgatg agcgggatat catatccttc
     1861 ccaggtgccg tcccggtcga tagcgttgag aaagatctcg ccgacgccgg ctttttccag
     1921 cgtcgtggcc cacccgacgg ggtaaaggcc cgaattcttc gtgccagaca ccgaatatgc
     1981 tttttgcttt ccgaaaaggt tccgtcctac atcgatgctc cctacaacgg cttgagcgcc
     2041 ataaatattg gctatccctt caataataga aagatcgttg tacaggaccg aattcagcac
     2101 gatcttctcg aaacccgaat cgaagacttt tttggcgttc tccagggtcc tgatccctcc
     2161 gccataggtc attggcatga acgcctctcc ggctatttcg gcgatctttt taaaatcggg
     2221 gtctcttttt tcccgcgtgg cagtataatc gagcaatacc agctcgtccg cctccttttc
     2281 gttgaagatc tttaccgtat tgatggggtc gccgacatat ttgggctcct tgaatttgac
     2341 ggtcttgtac agacctcctt ccttcagcaa cagaacgggt atgatacgta ccaggctcat
     2401 ggacggttat cctttgaaaa aattttccaa caccttcatc ccgaatttat ggcttttttc
     2461 ggggtggaac tgggttccga agatgttgtc tttccggaac gcgcaggcat attcgtaacc
     2521 atatacggca gtggccgtga cctcatcggg ttgcgtgaac cgaaaatgat atgaatgaac
     2581 gaaataaaac cgggattcgg ccggcagatc ggcccatagc gggctctcgc cgcaccggtt
     2641 gatctccctc cagcccatgt tcgggatttt cagcccattc atcgcggcct cttcgaatcg
     2701 aacggtgacc gcatccaccc accccagtcc gtcgacatcg ccttcttcgc tgtggccggt
     2761 catcaactgg gcgccgagac aaatgccgag tatcggccgc cgctccgcca gcaccaggcg
     2821 atcgagcagc tgtctggtgc cgccgctatt caggttctcc attccttttt tgaaatggcc
     2881 cacgccggga aggatcagct tgtcggcctc ctctatcctg gcgggatcgc cggagatgtc
     2941 agcctccacc cctattcgct tgcacatatt cttaatagag gtgagattgc cgagcccgta
     3001 gttgactata atggtcaaat ttttagattt ttctctctga atttcttgaa tttggatgca
     3061 gggatatatc catgatactc atgctcacaa cgaaattcga cacatttttc tttttcaagg
     3121 tctttgattt ttttggccgg cacccctccg aggacggcat atccatccgg gaaggaggtc
     3181 gtgaccaccg cgccggccgc gacgatggtc cagtcgccca gctcaacgcc cggcatgatc
     3241 ttggcgccgg cggcgatcca gcagtacctg cctatcttca ccggtttgca aatatgcttc
     3301 cgggagtcat atagatcgtg gttggcagta acgatcacca cattgggggc gatctgggta
     3361 taatctccga tcgtaagccc tcccctcccg gtaatataac agccccccat ggtccccgga
     3421 taggcgtcga tgccgacgag gatgttctcc acgtcatata ccttgctggt gaagtggacc
     3481 ggccaatagg cctttctgtt gccaccccaa tttaaaattt tctgtgtaaa ccagaactcg
     3541 aagttcacgt aattctggta gtccctggtc ctgtagatcc aacggaaata aggaatggtc
     3601 ttcgtcagca atacgatggg taactgaaga gggcgtttaa gaaaataggc gatcttttga
     3661 agcatgtcgg tactggttta ggcggatcca attgtccgcg atgcggtggg tattcagcgt
     3721 cagtgcgccc ttcagcactt ccttatagat ggaacgtcgc ccggcgcgca ggatcttttg
     3781 aacggttggc ttatccagcg gacaggacgc gtcggagaaa tacttcaatg tgatgtcgac
     3841 gtaggtgcgg atgaatttcc gatggctgtt catttcctgc gcatcatgct cgcggtacca
     3901 aacaatgcca tgcggcatga gcaccacagg gtatttctga cccagcctgt gccacatctc
     3961 gaaatccccc gccattctta tcggcgcgaa accacccacc gaacggaagg catccgtcct
     4021 gatgatcgaa gaaagcggcg ccttatggaa gagtcccggc cccaggtagt ggaactcata
     4081 agcttccttc gggctcagct ggaaggggaa cggttttgga acgtattgct ccagcgaaca
     4141 gagaccccat cccgccgacg ggaaggcttc catcatatcg accagcaagc caagccccca
     4201 gggataaata taatcgtcgg cgtctacata tttcaggtat ttgccattgg cataggcggc
     4261 ggcctggttc cggttgggat agtcgccgag gtttttctca ttgatatata ccctgaccct
     4321 cggatctttg tcggcatatc cgcgcgcgat ctccaccgta ttgtccttcg agccatcgtc
     4381 gacgatgatg agctcgaggt ttttgtagcc ggagttgagc acgctttcca ttgcgctgcc
     4441 gatgtatttt tcgcggttgt acgccgtcat cagcacgctg accaggggag tcatgccact
     4501 gtttgcagcc atgtggtaaa actattttgt aaagggtttc gcatcaggtt ttcgagcttc
     4561 gcggaatcga agatcgtgtc cccgctttcc agcttttcat cgatggccgg ccagggggtc
     4621 aggaccgtcg ggaccgaaaa ttttcgccca atggcatccg ccacttcctt caatgaataa
     4681 gtctccccgg caatattgaa agtattattc ttgctttggg ggtcctgcac gacccggaat
     4741 atctggcggc agatatcttc gacgtgcgtg aaagaccgtt tttgttcgcc ggtgc
//
LOCUS       DASYSV010000150         6933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2335301, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000150 DASYSV010000000
VERSION     DASYSV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335301"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VGQ51_04685"
     CDS             complement(<1..410)
                     /locus_tag="VGQ51_04685"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9035888.1"
                     /translation="MARSIVLFVLSWVCHSVVYANRVTGSVRDDKGNVLPYASVLVKG
                     TTRGVTAGSDGRYSIALSSGDYTLVCQYVGYGRQEKKISVRDSALVVDFSLSPQQLSM
                     AAVVVRPGGEDPAYAIIRHAIKRRRDYEAPLDSFS"
     gene            complement(418..762)
                     /locus_tag="VGQ51_04690"
     CDS             complement(418..762)
                     /locus_tag="VGQ51_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035889.1"
                     /translation="MVAKFFITTGLIALLSFIAGLFLPWWTIAVVAFLVSALIPQKPL
                     RAFFAGFLALFLLWGGMALGIDLANDSILSARVAAILPLGGSSLALIVVTALVGALVG
                     GGGSVTAAFLRK"
     gene            complement(866..3325)
                     /locus_tag="VGQ51_04695"
     CDS             complement(866..3325)
                     /locus_tag="VGQ51_04695"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEV9035890.1"
                     /translation="MRIVPFLISLILTIGLIVCLNRQWGVTKTPLLGQFLSPQHGFWQ
                     NAEPIGKDFGGDVKLEGMQGKGDVYFDENLIPHVFADSRTDACYIQGYLHARFRLWQM
                     EFQVYAAAGRLSEILGPGADNSILKYDRSMRRLGMVTAARAALAAMESDSSVKADCDA
                     YTAGVNAYISGLKASDLPLEYKLLNYMPEPWTNLKIALFIKYMAYDLAGADNDFELTN
                     ARSFFGKDEFARLFPAVQDSVDPIIPKGTLFQPPNVLPKMPRMADSLYLNSTATVTDL
                     ELKPEKDNGSNNWAVSGRKTNSGKPILCNDPHLSTNLPAIWYQMQIHTPSYNVYGVSF
                     PGAPYVIIGFNDSCAWGVTNAGRDVRDYYSIRFKDESRREYWFNGAWKDAPQRIDTIL
                     VKGREPFYDTVSTTVFGPVMFDPGFTGYSDVSSSHSYAVHWKPADTSDEIRTFSRFQS
                     MHNYSDYKAALNTFRSPGQNFAFADKAGEVALWQQGEFPAKWNEQGLFVMPGEDSSYM
                     WQGNIPEEENPHMVVSEHDRGFVSSANQLPADTSYPYYLGDDYPIYRGLVINRKLNNS
                     NSITAADMMAMQTSNYDIFAAMAKPMLLHNIQDLRLNEKAKNYLGIFSGWDLNDDPDE
                     VAPTIFRYWWAHLEKDIFDDEYSKTSLPMKRPYESTVLEALLRDSTWSFIDNVNTPEK
                     ETLRQVVTTAFLQAIPDLEEAASQGRLAWAKNKDTWARHLLRLPALSRMHLPIGGGVH
                     CINAAKQFHGPSWRMVVHLTTPTEAYGIYPGGQSGNPGSPYYDTFIDKWAAGLYNTLW
                     VMSADDVKNQRIRWVLHFSGT"
     gene            complement(3570..4667)
                     /locus_tag="VGQ51_04700"
     CDS             complement(3570..4667)
                     /locus_tag="VGQ51_04700"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEV9035891.1"
                     /translation="MPDEDFLGKRLEERRTQDALRRLRLPDGKVDFCSNDYLGVARNG
                     LLEKEMVHGDAHGSGGSRLLAGNYALIEETETAIAGFHSAPAGLIYNSGYDANVGLLS
                     CVPRRGDVILYDALGHASIRDGIRLSFAQSFGFAHNDCRDLERRLAAAEGGGTVFVVT
                     ESVFSMDGDLAPLYVMEELCGRYGAHLIVDEAHATGVIGAEGEGLVQRMGLQDRCFAR
                     VHTFGKAMGCHGAIVLGSETLREYLINFSRAFIYTTALPPASVRAIASAYRLAPGLGA
                     ERQQLQRLIERFCLAEIGFRRLGSGTPIQGVVVPGNAEVKALATRLQEAGLDVRPILY
                     PTVPRGGERLRIVLHSFNTEEEVDRLTGCLK"
     gene            complement(4703..6019)
                     /locus_tag="VGQ51_04705"
     CDS             complement(4703..6019)
                     /locus_tag="VGQ51_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9035892.1"
                     /translation="MRNFLPFLLLAFTNIAIAQTNTKYLIRAGSLFDSETGQFRDDMT
                     ILVTDNHITAVKPAKEVTRQEEQDRQVIDLSKYTVLPGLIDAHTHLLNREILHPGNDF
                     DGLDMARQLMMDGDAYRAIYGAARARAYLEAGITAVQDLGNSGEFADIALRRTIREGL
                     VAGPRMRCAGKGLSSQGGQMPGTIYKHQSVINDEYRIVRGPDDGVQAVRENITQGADV
                     IKIYSNNTPNNTYLSIAEMQAIVGEAHRYHIRVTAHATNNQAVYNAVVAGVDGIEHGY
                     EVADSTLELMAKKGVVLVPTDLDSVTFTQYVKLIAPSNPDEMMREIMGYRAYLGTRLR
                     RAIQKGVTIVAGSDDYVDFKLPFAEPSKRTLIGYHESGIAIPQVLQFATINASRQLNW
                     AGKIGVLKTGYLADIIAVDRDLEKNIDAILHVHFVMKDGKVITAVK"
     gene            6062..6676
                     /locus_tag="VGQ51_04710"
     CDS             6062..6676
                     /locus_tag="VGQ51_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HEV9035893.1"
                     /translation="METTPGVSQKNILGLQLPTDPRWVDLAAISLEDILTDHAWCEQK
                     AATSCISMIQRYPSRQRLVDDVSPIVTEEWGHFRLVLAELQKRGLRLGPQRKDEYVNR
                     LMAFEAKGGSEEQRFLDRLLICALIEARSCERFKRLSEGLEDPYLRQFYRRFMESEAG
                     HYHLFIELAESYIDAAVVRRRWAEWLEYEAGVIASLEVRGDRIH"
     gene            complement(6673..>6933)
                     /locus_tag="VGQ51_04715"
     CDS             complement(6673..>6933)
                     /locus_tag="VGQ51_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4918 domain-containing protein"
                     /protein_id="HEV9035894.1"
                     /translation="KELLRDAEPFVLKCIRRQLETMPTYPVCYCLGEGTNYKYFSRIN
                     EKHGFFHEIVPLPHPRWVMQYRRKRVGEVVEVYVSRLRAHVP"
BASE COUNT         1516 a         2101 c         1795 g         1521 t
ORIGIN      
        1 gaaaaggaat ccagcggggc ctcgtagtcc cgtcgccgct tgatggcatg acggatgatc
       61 gcataggccg ggtcttctcc gccgggtcgc accaccacgg cagccatgga caactgctgc
      121 ggcgagagac tgaagtcgac caccaacgcg gagtcgcgga cgctgatctt cttctcctgc
      181 cggccatagc ccacatactg gcatacgagg gtataatctc ccgaagaaag cgcgatgctg
      241 tatcggccat cactacctgc cgtcacgcca cgcgtggtcc ctttgacgag aacagaggca
      301 tagggaagaa cgttcccttt gtcgtcccgg acgctaccgg tcacgcggtt cgcgtagacg
      361 accgaatgac agacccagga gagtacgaat aatacaatac tgcgtgccat cgacacctca
      421 ttttcttaaa aaagccgccg tcacggatcc acccccgccg accagcgcgc ccacgagggc
      481 ggtgacgacg atcagcgcga gggaagaacc tcccagcgga aggatggccg ccacacgcgc
      541 ggacaggata ctatcgttgg ccagatcgat gcccagcgcc atgcctcccc acaacaggaa
      601 cagtgcgaga aacccggcaa aaaaagcgcg aagaggcttt tgcggtatca gcgcgctcac
      661 gagaaaagcc accacggcga tcgtccacca ggggagaaaa agtccggcga taaagctcag
      721 cagcgcgatc agaccggtcg ttataaagaa ttttgccacc atatgcgaat aaagtttatg
      781 tgctcgaatt ggtctgccgg aaaaatagcc caattcgagc aatctgccaa cgattgccct
      841 tcgggcgagc cggcccgtgg ccgtcttatg tacccgaaaa atgcaggacc caccggatcc
      901 gctggttctt cacatcgtcc gcgctcatca cccaaagcgt attgtaaagc cccgccgccc
      961 atttgtcgat aaaggtatca tagtacggac ttccgggatt gccactctgt ccccccggat
     1021 agatcccata ggcttccgtc ggtgtcgtca ggtgtacgac catcctccag ctggggccat
     1081 ggaattgctt ggcagcattg atgcaatgca ctccgccgcc gatgggcagg tgcatacgac
     1141 tcagagccgg cagcctcaac agatgacgcg cccaggtgtc cttattcttc gcccaggcaa
     1201 gccggccctg gctggcagcc tcttccagat cgggaatggc ctgcaggaag gcggtagtaa
     1261 ccacctgccg cagcgtttcc ttctcggggg tattcacatt gtcgataaaa gaccaggtgg
     1321 agtccctaag cagcgcttcc agcaccgtgc tttcataggg gcgtttcatg ggcaggctgg
     1381 tcttcgaata ttcatcgtcg aagatgtcct tttccaggtg agcccaccaa tacctgaaga
     1441 tggtgggcgc cacttcatcg ggatcatcgt tgaggtccca gccggagaag atgcccaggt
     1501 agttcttcgc cttttcattc aatcttagat cctggatatt gtgcagcagc atcggtttgg
     1561 ccatcgccgc gaaaatgtca taattgctgg tctgcatcgc catcatatcc gcggccgtga
     1621 tcgagttgct attgttcaat ttccggttga tgacgagccc ccggtagata ggatagtcgt
     1681 cgcccaggta atagggatag ctcgtatccg ccggaagctg gttggcgctg ctcacaaaac
     1741 cgcgatcatg ttcgctgacg accatatggg ggttttcctc ttcggggata ttaccctgcc
     1801 acatatacga gctgtcctcg ccgggcatca cgaagagtcc ctgttcgttc cacttcgcgg
     1861 ggaattcacc ctgctgccag agcgccactt ccccggcctt atcggcaaag gcgaaattct
     1921 gaccgggact cctgaatgtg ttcaacgccg ctttgtaatc ggaataatta tgcatcgact
     1981 ggaaacgact gaaggtcctg atctcatccg aagtatcggc gggtttccag tggacggcat
     2041 aggaatggct gctggataca tcggaatatc ccgtaaagcc ggggtcgaac atcaccggtc
     2101 cgaaaacggt ggtagacacc gtatcgtaaa aaggttcccg acccttgacc aggatcgtgt
     2161 cgatgcgttg cggcgcatct ttccacgcac cgttgaacca gtattcccgg cggctttcgt
     2221 ccttgaatct gatggaataa taatcgcgca cgtccctgcc tgcatttgtg acgccccacg
     2281 cgcagctgtc gttaaatccg atgatgacat agggcgctcc cggaaacgag actccataaa
     2341 cgttatacga cggcgtatgt atctgcatct ggtaccagat agcgggaaga ttggtgctca
     2401 gatggggatc gttgcaaagg atgggcttgc cgctattcgt cttcctgccg ctgaccgccc
     2461 agttattgct gccgttgtcc ttctccggct tgagctccag atcggtgacg gtagcggtgc
     2521 tgttgagata aagactgtcg gccatgcgcg gcatcttcgg caaaacattc ggaggctgga
     2581 ataacgttcc ttttgggatg atgggatcga cgctgtcctg caccgccgga aaaagcctgg
     2641 caaattcgtc tttgccaaaa aagctgcggg cattggtcag ctcgaaatcg ttgtcggcgc
     2701 ccgccagatc ataggccatg tatttgatga ataaggcgat cttcagattg gtccagggct
     2761 ccggcatata gttcagcagt ttgtactcga ggggaaggtc gcttgctttg agcccgctga
     2821 tataggcgtt tactcccgcg gtgtaggcat cgcaatccgc cttcacggag gagtcgcttt
     2881 ccatggccgc cagcgccgcc cgggctgcgg tcaccattcc cagcctccgc atgctccggt
     2941 catacttcag aatggaattg tccgccccgg gtccgagtat ctcgctgaga cgccccgcgg
     3001 cggcatagac ctggaattcc atttgccaca ggcggaatct cgcatgaaga tacccctgga
     3061 tatagcaggc atccgtccgg ctgtccgcaa agacatgcgg aatgagattc tcgtcaaaat
     3121 aaacgtcgcc tttcccctgc ataccctcca gcttcacgtc gccgccgaag tccttgccaa
     3181 tgggttccgc attctgccaa aatccgtgct gcggactaag gaattgaccg agaaggggtg
     3241 tcttcgttac accccattgt ctgttcagac aaacgatcag cccgatagtc aggatcagcg
     3301 agatcagaaa tgggacgatt ctcataccag tcgtattgat caactaaaat aagttatttt
     3361 ttggtaaagg gcccgccgaa ccgctttcgg ctgttcattc cctgttccgg taataccaca
     3421 aaatggtatt gtttggatgc atccttcccc tcatttttgc agtggtaaat gtatagtaac
     3481 acaagcgcgc aacgtactca acgtatcctt taatttagtt caggtggttc aggtaggcaa
     3541 taggccggtc tccaaaagag ccggcttatt cattttagac aaccggtcag cctgtccacc
     3601 tcctcttcgg tgttgaacga gtgcaacacg atgcgcaaac gctcgccgcc acggggcacg
     3661 gtggggtata gtatcggccg aacatccagc cccgcctcct gcaaccgtgt cgccaaggcc
     3721 ttcacttccg cattcccggg caccaccact ccctgtatag gcgtcccgct accgagtcgc
     3781 cggaagccga tttcggccag gcaaaagcgt tcgatcagcc gttggagttg ttgtctttcc
     3841 gccccgagcc ccggcgccaa ccggtaggcc gacgctatcg cccgcacgct ggccggaggc
     3901 aatgcggtcg tataaatgaa agccctcgag aaattgatca ggtattcccg caatgtctca
     3961 ctcccaagca cgatcgcgcc atgacatccc atcgccttcc cgaacgtatg cacccgcgca
     4021 aaacaccggt cctgcaatcc cattctttgc accagcccct ctccttccgc gccgatgaca
     4081 cccgtcgcat gcgcctcgtc gacgatcaaa tgggcgccgt aacgcccgca caactcctcc
     4141 atcacgtaca gcggcgccag atccccatcc atcgaaaaca cggattccgt aaccacgaag
     4201 acggtccccc cgccctccgc cgcggccaga cgccgctcca gatcgcggca atcattatgc
     4261 gcaaagccaa aagactgcgc aaacgacagc cttatcccgt cccttatcga tgcatggccc
     4321 agcgcgtcat acaggatgac atctccgcgc cgcgggacgc agctcaacaa cccgacattc
     4381 gcatcataac ccgaattata gatcaatccc gccggcgccg aatggaaacc agctatcgcg
     4441 gtttccgttt cttcgatcaa cgcatagttc cccgccagca gcctcgatcc gccgctgcca
     4501 tgcgcgtcgc catgaaccat ctccttttcc agcaacccat tcctcgcgac gcccaggtaa
     4561 tcgttggaac aaaaatccac cttcccatcg ggcagccgca accgcctcaa cgcatcctgt
     4621 gtcctgcgct cttcgagcct ctttcccaga aaatcttcat ccggcataac cgcattttcc
     4681 ggcaaacgta cacaaatccc gcctatttaa cagccgtgat taccttcccg tccttcatga
     4741 cgaaatgcac atgcaggata gcgtcgatat ttttttcgag gtccctgtcc acagcgatga
     4801 tatcggccag atagccggtc ttcagcacgc cgatcttacc ggcccagttc aattgccggc
     4861 tcgcattgat ggtagcgaat tgcagcacct gcggtatagc gatcccactt tcgtgatacc
     4921 cgatcagggt ccgtttggac ggttcggcga acggcagttt aaaatcgaca taatcgtcgg
     4981 agccggcaac gatagtcaca cctttctgga tggcccggcg caaacgtgtg cccaggtacg
     5041 cgcggtagcc catgatctcg cgcatcatct cgtcgggatt tgacggagcg attaatttga
     5101 catattgcgt gaaggtgaca ctatccagat cggtaggcac caacactacc cctttttttg
     5161 ccatcagttc cagtgtcgag tctgccacct catagccgtg ctcgatgccg tccaccccgg
     5221 ccaccaccgc attgtaaacc gcctggttgt tcgtcgcatg cgccgtcacg cggatatgat
     5281 accgatgcgc ttcacccact atcgcctgca tttccgctat gctcagataa gtgttgttgg
     5341 gcgtattatt cgaatagatc ttgatcacgt ccgcaccctg ggtaatattt tccctgacag
     5401 cctggacgcc atcatcggga ccccggacga tcctgtattc gtcattgatc accgactgat
     5461 gcttatagat cgtcccgggc atctggccgc cctgcgagga caggccttta cccgcgcacc
     5521 gcattcgcgg accggcgaca agaccttccc ggatggttct gcgcagggcg atatcggcaa
     5581 actcgcccga gttacccaga tcctgcaccg cggtgatccc tgcttcaaga tatgcccggg
     5641 cccgtgcggc gccgtagatc gcccggtagg cgtccccatc catcatcagc tgtctggcca
     5701 tatccaaccc atcgaagtcg ttgcccggat ggaggatctc cctgttaagc aaatgggtgt
     5761 gggcatcgat cagtcccggc aggacggtgt atttcgaaag atcgatgacc tggcggtcct
     5821 gttcttcctg cctggtcact tccttcgcag gctttacagc ggtgatgtga ttgtcggtta
     5881 ccaagatggt catgtcatcc ctgaactgcc cggtctcgct gtcgaacagg ctgcctgccc
     5941 tgatcaggta tttggtattg gtttgcgcaa tggcgatgtt ggtgaatgcc aggaggagga
     6001 atggcaggaa gtttctcatg atgcaaggtt ttgggctccc gaatttactt aattttggcc
     6061 catggaaacc acgcccggcg tcagccagaa aaacatcctc ggtctgcagc tgcccaccga
     6121 tccgcgctgg gtcgatctgg ccgctatctc attggaggat attcttacgg atcatgcctg
     6181 gtgcgaacaa aaggccgcca ccagctgtat ctcgatgatc cagcgatacc ccagccggca
     6241 gcgtctggtg gacgatgttt cacccatcgt caccgaagaa tggggtcatt ttcgcctcgt
     6301 actggcagaa ctgcaaaaaa gaggtctccg gctggggccg cagcgtaaag acgaatacgt
     6361 caaccggctg atggccttcg aagcgaaagg cggtagcgaa gaacagcgct tcctggatcg
     6421 cctcctgatc tgcgcgctga tcgaagcccg tagctgcgag aggttcaaac gtctcagcga
     6481 agggctcgaa gacccgtatc tccggcagtt ttaccggcgg ttcatggaaa gcgaggcggg
     6541 gcattaccac ctcttcatcg agctcgcaga atcctatatc gatgccgccg tcgtccgccg
     6601 acgctgggcg gaatggctgg agtatgaagc cggcgtcatc gcttccctcg aagtacgagg
     6661 cgaccgtatc cattaaggca catgcgcacg cagccgcgat acatacacct ccacaacctc
     6721 ccccacccgt ttgcgccgat actgcattac ccatctcggg tgcggcaacg gcactatctc
     6781 gtgaaaaaat ccatgctttt cattgatgcg gctgaaatac ttatagttgg tcccctctcc
     6841 cagacaataa cacaccggat aggtcggcat cgtttccaat tgacggcgga tacattttaa
     6901 aacgaaaggc tctgcatccc gcaacaattc ctt
//
LOCUS       DASYSV010000151         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1309524, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000151 DASYSV010000000
VERSION     DASYSV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309524"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VGQ51_04720"
     CDS             complement(<1..587)
                     /locus_tag="VGQ51_04720"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEV9035895.1"
                     /translation="MTKKIYFLSDFHLGAPDHAASLVRERRIVDFLQRIRADASEIFI
                     VGDLFDFWYEYRTVVPKGYVRILGKMAEIADSGIPIHFFTGNHDMWMSGYFEEELGIP
                     VHHEPKIFDFNGRRLLVGHGDGLGPGDHGYKFLKKIFRNRLCRGLFGILPPFVGVGIA
                     GWLSRRSRAVTGSDDARFLGPDKEWLIAYCREILQT"
     gene            complement(584..1222)
                     /locus_tag="VGQ51_04725"
     CDS             complement(584..1222)
                     /locus_tag="VGQ51_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEV9035896.1"
                     /translation="MKVSSAKENILKKIRQALSESTPIPFPQSEGNASVFQPSRQDLE
                     IEFAEQFAKLQGKFVFCVDHNELVSQLNALIATNGWKKIHCREQRLQDVLNTHSFGSF
                     FSSELAECDAAITGCEALVARTGSIILSSAGASGRTVSVYAPVHLCIAYTDQLVYDIR
                     DGLLGLKEKYHHQLPSMMSFATGPSRTADIEKTLVVGVHGPKQVYLFLVDRQ"
     gene            complement(1317..>3099)
                     /gene="ftsH"
                     /locus_tag="VGQ51_04730"
     CDS             complement(1317..>3099)
                     /gene="ftsH"
                     /locus_tag="VGQ51_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV9035897.1"
                     /translation="YIKQDSLNKRYYDKFKKSSSWPGGFSKEGPQFEFRVTDVKDYEK
                     MLRDYFAAHPNQEIPIVSKPEGEWFGPLLNFLLPLLIIVLIWVMLMRKMGGQAGAGGP
                     GGIFNIGKSKATLFDKGTKVNITFSDVAGLDEAKVEVMEIVDFLKNPKKYTALGGKVP
                     KGALLVGPPGTGKTLLAKAMAGEAQVPFFSMSGSDFVELFVGVGASRVRDLFKQAREK
                     SPCVIFIDEIDAIGRARGKNAIMSNDERESTLNQLLVEMDGFGGDSGIIVLAATNRPD
                     VLDTALLRPGRFDRQISIDKPDLKGREAIFKVHLKPIKISSKLDIHKLAEQTPGFAGA
                     DIANVCNEAALIAARKGKETVEMEDFQDAVDRVIGGLEKKNKIISPDEKRIIAYHESG
                     HAICGWFLEHAYPLLKVTIVPRGVAALGYAQYTPKEQYLYNTDQLFDQICMTLGGRAS
                     EELNFGKISTGAQNDLQQITKIAYSMVTVYGMNEKVGNISFYDPQQENTFTKPYSEET
                     SKLIDEEVRKLIDKAYEATKRLLTAKSREVRMLAEALLEKEVLFQSDVEALIGKRPYE
                     EKRALDVSEHENGVPPAVVVPPTTEQV"
BASE COUNT          651 a          865 c          822 g          761 t
ORIGIN      
        1 gtctgcagga tctcccggca ataggcgatc agccattcct tgtccggacc caggaaccgg
       61 gcatcgtcag agcccgtaac ggcccggcta cggcggctca gccagccggc gatcccaacc
      121 ccgacaaacg gcggaagtat cccgaaaaga ccgcgacaaa gcctgttgcg gaatatcttt
      181 ttgaggaact tgtatccatg gtcgccggga ccgagtccgt ccccgtgtcc gaccagcagg
      241 cgtcgcccgt tgaaatcaaa aatcttcggt tcatgatgca ccggaatacc cagctcttcc
      301 tcgaaatacc cgctcatcca catgtcatgg ttaccagtga agaaatggat cggaatgccc
      361 gaatcggcaa tctcggccat cttgccaaga atacggacat accccttggg gaccaccgtc
      421 cggtattcat accaaaaatc gaaaagatcg ccgacgataa agatctccga cgcatccgcg
      481 cgtatccgct ggaggaagtc gacgatacgc cgttcacgca ccaggctggc ggcgtgatcg
      541 ggagcgccca gatgaaaatc ggaaaggaaa tatatctttt ttgtcattgc ctgtctacca
      601 ggaaaaggta tacctgtttt gggccatgga cgccgacgac cagggtcttc tcgatatcgg
      661 ccgtccggct gggcccggtt gcaaagctca tcatcgacgg cagctggtga tggtattttt
      721 ctttcaatcc gagcagaccg tcacggatat cataaaccaa ttggtcggtg taggctatgc
      781 agagatggac gggcgcataa acactgaccg tccggccgct ggcgcccgcc gagctgagaa
      841 taatactacc cgtccgcgct accaatgctt cgcagcccgt aatagcggca tcgcattccg
      901 ccagttcgga ggaaaaaaag ctgccgaagg aatgggtatt caggacatcc tgcagccgtt
      961 gttcgcggca atggatcttt ttccagccat tggtcgcgat gagggcattc agctgcgaga
     1021 cgagctcgtt atgatcgacg cagaagacga attttccctg cagcttcgcg aactgttcgg
     1081 cgaattcgat ctcgaggtcc tgtctggaag gctggaaaac ggaggcattg ccttcgcttt
     1141 gcggaaaagg aataggcgtt gattcactca acgcctgtct tatctttttg agaatatttt
     1201 cctttgcgga ggataccttc atcgggagtt tatgtttttt tcaatggtcc gatgcagtcg
     1261 ctgtgctcgt gtcattatcg cgattttaat tcggggctat gccgccgcgg caattattat
     1321 acctgttcgg tcgtcggggg aaccaccacc gccgggggaa cgccattctc gtgctcggaa
     1381 acgtcgaggg ctcttttctc ctcgtaagga cgtttgccga tgagggcttc cacatcactc
     1441 tggaacagga cctctttctc cagcagcgct tccgccagca tcctcacttc ccggcttttc
     1501 gccgtcagga gtcgtttggt cgcttcatat gctttatcga tcagcttgcg cacctcctcg
     1561 tcgatgagct tggaggtctc ctccgaatag ggcttggtaa aggtgttttc ctgctgtggg
     1621 tcgtaaaagc tgatattccc gaccttttcg ttcatgccgt agacggtcac catcgagtag
     1681 gcgatcttcg tgatctgctg cagatcgttc tgtgcgcccg tcgatatctt cccgaaattg
     1741 agctcttcac tggcacgacc gccgagggtc atacagatct ggtcgaacaa ctgatcggta
     1801 ttgtagagat attgttcctt aggagtatac tgggcatacc cgagtgcagc gacgccgcgc
     1861 ggcacgatgg tcaccttcaa taatggatag gcatgttcga ggaaccagcc gcagatggcg
     1921 tgtcccgact catgataagc gatgatccgc ttctcatcgg gggagatgat cttgttcttc
     1981 ttctccagac cgccgatgac cctgtccacg gcatcctgga agtcttccat ttcaaccgtc
     2041 tctttaccct tacgggcagc gatgagcgca gcctcgttgc agacgttggc gatatccgct
     2101 ccggcgaaac cgggcgtctg ttcggccagt ttatgtatat ccagcttgct ggagatcttg
     2161 atgggcttga ggtgtacctt gaatatcgcc tctctgccct tcagatcggg tttgtcgatg
     2221 gaaatctgac ggtcgaacct gccgggacgt agtaacgcag tgtccaatac gtcgggtctg
     2281 ttggtcgcgg ccagtacgat aatgccgctg tcgcctccaa agccatccat ttctacgagt
     2341 aactggttga gtgtgctttc cctttcatca ttgctcatga tcgcattctt tccccgggcg
     2401 cgaccgatgg cgtctatctc gtctatgaag atgacacagg gagatttctc gcgggcctgt
     2461 ttgaacagat cgcgaacgcg gctggcgccg acgccgacga acaattccac gaagtcactt
     2521 ccgctcatgg aaaagaacgg cacctgtgct tcgcctgcca tcgcttttgc cagcagggtc
     2581 ttaccggttc cgggaggacc taccaaaagg gcccccttgg gtaccttacc tccgagggca
     2641 gtatattttt tggggttctt caggaaatct acgatctcca tgacctccac cttcgcctcg
     2701 tccaaaccgg caacatcgct gaaggtaatg ttcaccttgg tgcctttgtc gaagagcgtg
     2761 gccttggact taccgatatt gaagatgcct cccggtccac cggctcctgc ctggccgccc
     2821 atttttcgca tgagcatgac ccagatgagc acgatgatca gcaacggcag caggaaattc
     2881 agcagcggac cgaaccattc tccttccggt ttcgagacga tgggaatctc ctgattggga
     2941 tgagccgcga aataatcccg cagcatcttc tcataatcct ttacgtcggt gacccggaac
     3001 tcgaactggg ggccttcctt gctgaaaccg ccgggccagg agctggattt cttgaatttg
     3061 tcgtagtagc gtttgttgag actgtcctgt tttatatag
//
LOCUS       DASYSV010000152         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1182890, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000152 DASYSV010000000
VERSION     DASYSV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182890"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1678
                     /locus_tag="VGQ51_04735"
     CDS             119..1678
                     /locus_tag="VGQ51_04735"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEV9035898.1"
                     /translation="MRKLLVITALFLNIHSSRGQQAATQRQTVTPQQITTPDQTVTPQ
                     QTVTPPMGWNSYNCFGSAVHEDEVKENASYMAQYLKSYGWRYIVVDFLWSYDNPPGSF
                     IGNPFQQRLQDGSYIPWLEMDKWGRLTPDIHKFPSAFGGKGFGPLAAYVHSLGLKFGI
                     HVMRGIPRQAVWAHSPVLGTNGITADQVADTNSTCPWMNHMYGLDMDKPGAQEYLNSL
                     LRLYAGWGVDFIKVDDISRPYHSREIEGYRKAIGSCGRPIVLSLSPGETPLKDSAHVR
                     RYADMWRMADDFWDSWKQLLKMFDYARQWEGVGGDGHWPDCDMMQIGRVSKRGPVGPE
                     RWSRLTEDELRTHVTFWCIYRSPLMLGGNLPENRPFETQLLTNPEVLAADQQGANPRQ
                     IFKEDGKMVWVSTAPGPTASGAIPTASGANSFYVALFNIGDSAQDVRVDFASLGLKGK
                     VSVRDCWKRSDAGVFEHGYGQHLPAHGATLIRVSPLIHRPNRMSIAPVPSTNRGTGYS
                     FNSSDNSSSTL"
     gene            complement(1951..2304)
                     /locus_tag="VGQ51_04740"
     CDS             complement(1951..2304)
                     /locus_tag="VGQ51_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEV9035899.1"
                     /translation="MHTIKIKRIYEPSAEADGYRVLVDRLWPRGIKKESAHIHIWEKD
                     VAPSKELRKWFGHDPEKWDGFKKKYLAELKGSDAIDHLVGELRSHKTVTLLYGAHDEE
                     HNNAVVLREFLVKKV"
     gene            complement(2515..>3010)
                     /locus_tag="VGQ51_04745"
     CDS             complement(2515..>3010)
                     /locus_tag="VGQ51_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEV9035900.1"
                     /translation="QMATGQAPNLGALNAAAGIIRSFVEDYHEKQEEDYLFTRFRKAG
                     QLVDLVDILLRQHNAGRKITDQILQLTKGPSVNAAEAPQLNGLLTSFNTMYRPHEARE
                     DTVLFPAFRKLVSRHEYDSLGEEFEKNEQKHFGKDGFETMVGRVADIEKQLGIYDLNQ
                     FTPS"
BASE COUNT          700 a          812 c          790 g          708 t
ORIGIN      
        1 tgggaaaaag gcccgatggg ggcggggctt cgtttggtaa aaactgcttc tttcattcaa
       61 atggagtttc ctgaaaataa taaaaattaa tcgacatctt tgaacaaata aatttgcgat
      121 gagaaagctg ctggtcatta ccgctctttt cctgaatatt cacagctccc ggggacagca
      181 agccgctacc cagcggcaaa ccgtcacgcc gcagcaaatc actaccccgg atcagaccgt
      241 cacgccccag cagaccgtca cgccgccgat ggggtggaac agctataact gttttggctc
      301 ggcagtgcat gaggacgagg taaaggagaa cgcttcctat atggcgcaat atcttaaatc
      361 ttacggctgg cggtatatag tagtcgattt tctttggtcg tatgacaatc cccccggcag
      421 tttcatcgga aatccttttc aacagcggtt gcaggatggc tcctatattc cctggctgga
      481 gatggataaa tgggggcggc tgacgccgga catccataaa ttcccttcgg ctttcggagg
      541 caaaggcttc ggcccgctgg ccgcttacgt ccactccctg ggattgaaat tcggcatcca
      601 tgtgatgagg ggcattccac ggcaggccgt atgggcgcat tcccccgtcc tggggaccaa
      661 cggcattacc gccgaccagg tggccgacac caactctacc tgtccgtgga tgaatcacat
      721 gtatggtctg gacatggaca aaccgggtgc gcaggaatac ctcaattccc tgcttcggct
      781 atatgcggga tggggcgtcg atttcatcaa agtggacgac atctcgagac cttatcattc
      841 gcgggagatc gaaggttatc gaaaagctat cgggtcctgc gggagaccca ttgtcctcag
      901 cctctccccc ggagaaacgc cgttgaagga ttccgctcac gtccgtcgct acgcggatat
      961 gtggcggatg gccgatgact tctgggacag ttggaaacag ctgctgaaaa tgttcgatta
     1021 tgccagacag tgggagggcg tcggcggtga cggtcactgg ccggattgcg atatgatgca
     1081 gataggaagg gtgtccaaac gcgggcccgt aggacccgaa cgctggagcc gtctcacgga
     1141 agacgaactc cggacccatg tgactttttg gtgtatctac cgctcgcccc tgatgctggg
     1201 tggcaatctg cccgagaacc gcccgttcga aacacaattg ctcaccaacc cggaggtgct
     1261 ggccgccgat caacaaggtg cgaacccccg acagatcttt aaggaggatg gtaaaatggt
     1321 atgggtgtcc accgcaccgg gccccactgc ctccggcgcc atcccgactg cctctggcgc
     1381 caattcattt tatgttgcgc ttttcaacat cggtgattcg gcacaggatg tacgcgtaga
     1441 tttcgcttcc cttgggttga agggtaaggt gtcggtgcgc gattgctgga aacggtccga
     1501 tgcaggcgtc ttcgaacatg gttatggcca gcatctcccc gctcacgggg ccacattgat
     1561 cagggtaagt cccctgatcc atcggccaaa ccggatgagc atcgcgcccg tacccagcac
     1621 gaacaggggt accgggtatt ctttcaatag cagcgacaat tcatcgtcca ctttatgaca
     1681 aaagatatat tgcagggctt cctgcctgtc atcccgtccc ggattggcgg caggctttga
     1741 aaaagtatcc gcctggatgg accgaagaaa ggagagcgag gacgagttga aataatataa
     1801 ccctgcattt tcttcgccca gcaccaaaag aagaaaccgt aagccgcgag agttcatgac
     1861 gcaacaattt aaaaggagac ggccattcgg agttagcgta aattccttcg caatgattaa
     1921 aatgaagtta gaaaggaatt agaaaatctg ttaaactttc ttaacaagga attcccgcag
     1981 caccaccgca ttattgtgct cctcatcatg cgcgccatac aacaaggtca ccgtcttatg
     2041 tgaccgcaac tctcccacga ggtgatcgat ggcgtccgac cccttcagct cggcgaggta
     2101 tttctttttg aaaccatccc atttctccgg gtcatgacca aaccattttc gcagctcctt
     2161 cgaaggagcg acatccttct cccagatatg aatatgcgca gattcttttt ttatccctct
     2221 cggccagagc ctgtcgacca gcacccggta cccgtcggct tcagcggacg gttcatatat
     2281 ccgcttgatc ttgatggtat gcataataga tcgttttcaa aaaaaaccgc cccaggatta
     2341 gctgaggcgg catatcattg aagcaggctg ccatgcggcc ttacggggcc tgtgctggta
     2401 acccggcttc agcgcgtgaa caaactaaag ttaaatcatt ctcccggaaa aaacaatagc
     2461 gggaccgggg aattatacgc caatttttca gagacgcccg attcttcgaa ggttttatga
     2521 tggtgtgaat tggttgagat cgtaaatacc caattgtttt tcgatatcgg ccacccttcc
     2581 gaccatcgtt tcgaaaccgt ccttgccgaa atgtttttgc tcattcttct cgaactcttc
     2641 cccgagggag tcatattcat gccgggagac cagcttccga aaggccggga acaacaccgt
     2701 gtcctcgcgt gcttcgtgcg gccggtacat ggtattgaag gaggtaagca ggccgttgag
     2761 ctggggcgcc tctgcggcat ttaccgatgg ccccttcgtc agctgtagaa tttgatcggt
     2821 gatcttccgt ccggcattat gctgccgcag caggatatct acaaggtcga ccagttgccc
     2881 ggcctttctg aatcgggtaa agaggtagtc ttcttcctgt ttctcgtgat agtcttcgac
     2941 gaaactgcgg atgataccgg cggcggcgtt caacgcccct aaattcggcg cctgtcccgt
     3001 cgccatctgc
//
LOCUS       DASYSV010000153         5263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_801430, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000153 DASYSV010000000
VERSION     DASYSV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801430"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VGQ51_04750"
     CDS             complement(<1..572)
                     /locus_tag="VGQ51_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035901.1"
                     /translation="MKTYLYLFVFAGCAFSASAQNKTAHDPDDSIVVEKSKAASAASA
                     APWHWQDVDGDYGPLPASVHVYRTADSLDGRPQVAYYVSAPLGDKELGFTAQVGYGKR
                     YTPAQYYGQEGHPLLVINTSFFSFATNGNLNLVMRDGKMLAWNQPSRKGKKDSIFHYT
                     TRSALGITAGRKADVAWIFTDTTQPLPYAF"
     gene            complement(583..1335)
                     /locus_tag="VGQ51_04755"
     CDS             complement(583..1335)
                     /locus_tag="VGQ51_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9035902.1"
                     /translation="MIDAIIIDDESHCIRHLSGLLSASCGSTIRLADAASTLEDGWRS
                     ILRLKPQLVFLDIQLHDETGFDLLDRIPVIDFDIIFVTAYEQYAIRAFKFSAIDYLLK
                     PVDPDDLLQAVSRLQQRRTKEDTARRFETLLDNLRNGTGLPRRISVPTIKGMEFLPVQ
                     DIIRCESHVNYTTIFLTNGQKLTVARTLKEFEELLEEHHFYRVHNSHLINLACIKSYH
                     KGKGGSVRMSDNTEIEVSTRRKDELLKKLAEL"
     gene            complement(1342..3159)
                     /locus_tag="VGQ51_04760"
     CDS             complement(1342..3159)
                     /locus_tag="VGQ51_04760"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV9035903.1"
                     /translation="MAKRLLLIALFVRAIAGFGQQTAAADSLLRVIRAHPARDSNRVT
                     SLLRLADAIVYTDAPTAMRYADEALAISEEQHWTKGIALSWRQKGNAYYVFSDNPAAM
                     DCYIKALNSARPLAIRELDASLFNNLANIYSDEKQFDKALNYYGQFLDAARELRSGKE
                     ETIALVNMGTVYSEIKEHGKAISCGLQALEISRRDKIDYFIPIILNNLGVAYVKKGDA
                     DSALDYFRQSITLGEKFDNKDARGSSLEETGKIYLLRGDYSNADRSIRQALAIGQELH
                     SAERLTDAWQSLSQLYEKQHDYKKALDAYRQYILFRDSVSNDEKRSSITKKEMQFDFE
                     RKESATKAASDKQAAVAQAEIRRQELIRKGMVAGVTFLLMAGLCGFLLYKRRRDADMR
                     RKESDLKALVAETEMKVLRLQMNPHFIFNSLNSISDYISRNETTTATYFLSKFAKMMR
                     QVLEHSEKSEITLAEDLQVVETYVQLETLRLRNELRYEIVIDKDIDPEVTMVPPLIAQ
                     PFVENSIWHGLSGRQGQLSIRIHRSDGMMCYIVEDNGIGRKEAAKRTPAGKRSMALRI
                     TQDRLDILNQTRGKGAGLEIFDLSDGTRVELRLPLELNY"
     gene            3295..3864
                     /locus_tag="VGQ51_04765"
     CDS             3295..3864
                     /locus_tag="VGQ51_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035904.1"
                     /translation="MKRLLLLLSVTGLALAGHTQEFQKGTNILSAGIGLGSSILSYSG
                     ASQTPALSLQYERGVWDIGGPGVISLGGYVGYKGYSYSAGEQNYNYSENWHYTVVGVR
                     SAYHYNGLDAKKLDLYGGLMLAYNLLSFSYSDNTGSGTIAGSSGSYGSTVGLSLYVGA
                     RYFFADHFAVLGELGYGVAYLNVGIAAKF"
     gene            3974..4237
                     /locus_tag="VGQ51_04770"
     CDS             3974..4237
                     /locus_tag="VGQ51_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035905.1"
                     /translation="METNGAQLPQAELFWYDSTGVGYLTAGTMSDANGHVSLSGVVAD
                     AKVTITDLTATYVKVTFSGTVYRPDFAVSRVITNGEFYLKRNN"
     gene            complement(4242..5222)
                     /locus_tag="VGQ51_04775"
     CDS             complement(4242..5222)
                     /locus_tag="VGQ51_04775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HEV9035906.1"
                     /translation="MEFQLPISPDPLPAPIRYGDKLLLIGSCFTEHIGGALADWKFDT
                     LQNPNGILFDPASVASSLESYVAPESYGSGDLFFANELWQSWKHHGIFSGVDRDEVLQ
                     GINASQESAHLFLKKADWLIITLGSSFSYRLVGDGRPVANCHRVPQQQFRKHLMTIEE
                     IVTALDGCLHRLFYFNPNLRIIFTVSPVRHIRDGVVENNRSKARLIESVHHLVNKFDR
                     LWYFPAYELVIDVLRDYRFYGIDMVHPNYAATQFVLEHFTQFGIDAESRSVLEHVKQL
                     VAARRHRPRHPSTAAHARFMREHYEKAAALGRQYPFLHLQEELEYFSAGK"
BASE COUNT         1149 a         1509 c         1374 g         1231 t
ORIGIN      
        1 tcgaacgcat agggcaacgg ctgcgtcgta tccgtaaaga tccaggccac atccgcctta
       61 cggccggcgg taatgcccag tgcgctacgg gtcgtataat gaaagatcga atcctttttt
      121 ccttttcgcg aaggttgatt ccacgcgagc attttcccgt cccgcattac caggttcaga
      181 ttgccgttgg tggcgaatga aaagaaggac gtattgatca ccagcagcgg atgcccttcc
      241 tgcccgtagt attgggccgg cgtataccgc ttgccatagc ccacctgcgc ggtaaagccc
      301 agttccttgt cgcccagcgg cgccgagaca taataggcca cctgcggacg tccatccagc
      361 gaatcggccg tcctgtagac atgcaccgat gccggtaaag gaccatagtc gccgtccacg
      421 tcctgccaat gccagggagc cgcggaggcc gcggaagccg ccttgctttt ctctaccact
      481 atcgaatcat cggggtcatg cgccgtctta ttctgcgcgc tcgcggaaaa agcacatccc
      541 gcaaatacga ataaatacaa atatgttttc atcgtcggca aattacaact ccgccagctt
      601 tttcagcagt tcatcttttc tccgggtgga tacttcgatc tcggtattgt ccgacatacg
      661 tacggatccg cccttgccct tatgatagct tttgatacag gcgagattga tgaggtgaga
      721 attgtgaacg cgataaaagt gatgttcctc caacaattcc tcgaattcct tcagcgtcct
      781 ggcaacggtc agcttctgcc cgtttgtcag gaatatagtc gtgtagttga catggctttc
      841 gcagcggata atgtcctgca ccggcaggaa ctccatcccc ttaattgtgg gcacgctgat
      901 acggcgggga agccctgttc cattgcgaag gttatccagc agcgtttcga atcgtcgggc
      961 ggtatcttct tttgtgcgcc gctgctgaag ccgtgacacg gcctgaagca ggtcgtcggg
     1021 atccaccggc ttcaacagat aatcgattgc gctgaacttg aaggcccgta tcgcatattg
     1081 ctcgtaggcg gtgacgaaga tgatgtcgaa atcgatcacc gggatccggt ccagcagatc
     1141 gaaacccgtt tcgtcatgca gctggatatc caggaatacc agctggggct taagccgaag
     1201 aatactcctc catccgtcct ccagcgtcga agccgcgtct gccagccgga tcgtcgaacc
     1261 gcatgacgcg gaaaggagtc ccgacaggtg gcggatacaa tgcgattcat cgtcgataat
     1321 aattgcgtcg atcatgaatg cttaatagtt taattccaat ggaagacgca gctccacccg
     1381 cgttccatcg gaaagatcga atatctccag acccgcccct tttcccctcg tctgattgag
     1441 gatatcgagc cggtcctggg tgatacgaag cgccatcgaa cgttttccag cgggtgtgcg
     1501 ctttgccgcc tccttacgtc cgatgccgtt gtcctccacg atatagcaca tcattccatc
     1561 gctccggtgg atgcggatcg aaagttgacc ctgtcttccc gaaagcccgt gccagatgct
     1621 gttttcgaca aagggctgcg cgatcagcgg aggcaccatc gtaacctccg gatcgatatc
     1681 cttatcgatg acgatctcat atcggagttc gttccgcagc cgcagcgtct ccagttggac
     1741 atacgtctcc actacctgca ggtcttccgc cagcgtgatc tcgctttttt cggaatgttc
     1801 cagcacctgc cgcatcatct tcgcgaattt gctcaggaaa taagtggctg tcgtcgtttc
     1861 attccgggat atatagtcgc tgatcgaatt caacgaattg aaaatgaaat gcgggttcat
     1921 ctgcagccgc agcaccttca tctccgtctc ggcgaccagc gccttgagat ccgattcctt
     1981 tcggcgcatg tcggcatccc gccgccgttt gtataacagg aagccgcaca accccgccat
     2041 caacaggaag gtgacgcccg ctaccattcc cttccggatc agctcctgcc ggcggatctc
     2101 cgcctgcgcg accgccgcct gcttgtcact ggccgccttc gtggcggatt ctttccgctc
     2161 gaaatcgaat tgcatttcct ttttggttat cgacgacctt ttctcgtcat tcgaaacgct
     2221 gtcccggaaa aggatatatt gccggtaagc atccagcgcc tttttataat catgctgttt
     2281 ctcatacagc tggctgagac tctgccaggc atcggtcagc cgttcggccg aatgcagctc
     2341 ctgtccgatg gccagcgcct gacggatact tctgtccgca ttcgaatagt caccgcgcaa
     2401 aaggtatatc ttacctgttt cctccagcga ggacccccgg gcatccttgt tgtcgaattt
     2461 ttcaccgagc gtaatactct gccggaagta gtccagtgcg ctgtcggcat cccctttctt
     2521 gacataggcg acgcccaggt tgttcaaaat gatcggaatg aaataatcga tcttgtcccg
     2581 ccgcgagatt tcgagcgcct gcaatccgca ggagattgcc tttccgtgct cttttatctc
     2641 ggaatacacc gtccccatat tgaccagcgc aatggtctcc tcctttccgg atcgcaattc
     2701 cctggcggcg tccaggaatt gtccgtagta attcagcgcc ttgtcgaatt gcttttcatc
     2761 gctgtaaata ttcgcgagat tgttgaagag actggcatca agctcccgga tcgccagcgg
     2821 cctggcgcta ttcaaggctt tgatatagca gtccatcgcg gcgggattgt cggagaatac
     2881 gtaatacgca ttccccttct gacgccagga gagcgcaatc ccttttgtcc aatgttgttc
     2941 ttcggagatc gccaatgctt cgtcggcata cctcatggcc gtgggtgcgt cggtatagac
     3001 tatcgcatcc gccagcctga gcaggctcgt tacccggttg ctgtcccggg cgggatgcgc
     3061 gcggatgaca cgcagaagag agtcggcggc agctgtttgc tgcccaaaac cggcaatggc
     3121 gcgcacaaac agcgcaatca gcagtagtct tttggccatc ggtagggggt gcttggggcc
     3181 ataaaagata cacaaatttc accgctttcg gctcaaaaaa taaataccgg tcggcagaga
     3241 ataaatgccg cttgcctcgc cttcaccaca cggctaattt ggtcaaaaaa aattatgaaa
     3301 aggctactac tcctcctatc cgtgaccggt ctcgccctcg ccggccatac acaggaattc
     3361 caaaaaggga caaatattct cagcgcaggc attggtctcg gaagctcgat cctcagctat
     3421 tcgggcgcat cgcagacacc cgccctcagc ctgcagtacg aacggggtgt atgggacatc
     3481 ggagggccgg gcgtcatcag tctcggcggc tatgtcggct ataagggcta cagttactcg
     3541 gccggcgagc agaactacaa ctacagcgag aactggcatt ataccgtcgt cggcgtccga
     3601 agcgcctatc attacaatgg tctcgacgca aagaagctgg acctctatgg cggtctgatg
     3661 ctggcttaca atctcctcag cttttcctac agtgataaca ccggctccgg gaccatcgca
     3721 ggcagcagcg gcagttatgg cagtaccgtc gggctcagcc tttatgtcgg cgcccggtat
     3781 ttcttcgccg accatttcgc cgtgctgggc gagttgggct atggtgttgc atatctgaat
     3841 gtagggatcg cggctaaatt ttaaatcgtt gtcatgaaga tccatctgtc ccgtcaatta
     3901 caccagcgac cccaacgcca cgctttcgtt tctcagcgga gagggcgtcg cctacgacga
     3961 cccgaacaag gcggtggaga ccaatggcgc ccaactcccg caggccgagc tgttctggta
     4021 tgattccacc ggtgtaggct acctcacggc aggtacgatg tccgatgcca atggccatgt
     4081 ttccttatcc ggtgtcgtcg ccgatgcaaa ggtgaccatc accgatctga ccgccaccta
     4141 tgtcaaggtt acattttccg gcacggttta tcgtcccgac tttgccgtgt ccagggtcat
     4201 aacgaatgga gaattctatt tgaagcgcaa taattagcag gttacttccc ggcggaaaaa
     4261 tattccagct cttcttgcag gtgaagaaat ggatattgcc ggcccagggc ggctgccttt
     4321 tcataatgct cgcgcatgaa cctggcgtga gcggccgtcg agggatgtct gggccggtgc
     4381 cgccgggcgg caaccagctg tttcacatgc tcgagcacgg agcgcgattc ggcatcgatg
     4441 ccgaattgtg taaaatgttc gagtacgaat tgcgtggcgg catagttggg atggaccata
     4501 tcgatgccgt agaaccggta atcccgaagc acatcgatca cgagctcata agcggggaaa
     4561 taccacaggc ggtcgaactt attcacgaga tgatgcaccg attcgatcag cctggccttg
     4621 ctgcggttgt tctccaccac tccatcgcgg atatgacgca cgggggatac ggtaaagatg
     4681 atccgcagat taggattgaa atagaaaagc ctgtggagac agccgtccag cgcggtaacg
     4741 atctcctcga tggtcatcag gtgcttgcgg aattgctgtt gcgggacccg gtgacaattg
     4801 gctaccggtc gtccatcccc gacaagacga taagagaacg agctgcccag cgtaatgatc
     4861 aaccaatccg cctttttcag aaataggtgc gcagactcct gcgacgcgtt gatcccctgc
     4921 agtacctcgt cgcgatcgac tcccgaaaag atcccatgat gtttccagct ctgccagagt
     4981 tcgttcgcaa aaaacaggtc cccggaccca tacgattccg gcgccacata ggattcaagg
     5041 ctcgacgcca cgcttgcagg atcgaaaagg atgccattgg ggttttgcaa ggtgtcgaac
     5101 ttccagtcag ccaacgctcc cccgatgtgt tcggtaaaac acgagcctat cagcaatagc
     5161 ttgtccccat accggatggg agccggcagg ggatcgggcg atatgggcag ttgaaattcc
     5221 atttatacga atacctcccc cgccagccgc aggctctcgc gca
//
LOCUS       DASYSV010000154         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1436955, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000154 DASYSV010000000
VERSION     DASYSV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436955"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VGQ51_04780"
     CDS             <1..700
                     /locus_tag="VGQ51_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEV9035907.1"
                     /translation="EQDVVLILSKSGESPEIKMLLSLIRGFGNTLIGMVGNLQSVLAK
                     QSDIVLNTTVAQEACTNNLAPTSSTTAQMVMGDALAVTLMEMRGFGSEDFARFHPGGT
                     LGKRLYLRVEDVYTHNERPRVAVDTMLREVIIEITSKRLGAAAVTEEDGTLLGIITDG
                     DLRRLLEKEGVREGLKAGDIMTRSPKTVEADALAVEALDLMRIHDITRLVVTNGRRYL
                     GFIDLNNLIREGII"
     gene            705..1787
                     /locus_tag="VGQ51_04785"
     CDS             705..1787
                     /locus_tag="VGQ51_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEV9035908.1"
                     /translation="MNRHHYVVIMAGGIGSRFWPMSRQDYPKQFLDILNTGRTLIQWT
                     FDRFASFIPVENIYVVTSQEYGDIVKEQLPQLPVSNILGEPSRKNTAPCIAYISFKLQ
                     QSDPKASLIVAPADHLILDKTAFTKVCLEALSFVGKHNAFITLGIKPTYPNTGYGYIQ
                     YEQHSVSDNVYKVKTFTEKPNLELAKTFMASGDFLWNAGIFVWQVKNVIHAFEKYLPE
                     MYDVFAAEKDKFNTPAERSALQTIYPLCTNISIDFGIMEKADNVYVIPSSFGWSDLGT
                     WNSAYENLKKDHLENAVAGDNVIVVDASRCMVHAPNDKLLVVQGLDKFIVVDTSDVLL
                     ICHRDKEQEIKEYVAEVKRNKGDQYL"
     gene            1821..2540
                     /locus_tag="VGQ51_04790"
     CDS             1821..2540
                     /locus_tag="VGQ51_04790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV9035909.1"
                     /translation="MSPYPLHTILFLDIETVSQYPDFNRVPGEWQQLWRLKAEALNRD
                     KTPATPEELYGRAAIYAEFGKVICISCGVLSGPPADRKLSLKSFYGDDEKELLQQFYE
                     LLHKWGSEGQKYLCAHNGKDFDFPYLCRRMVIHGMKIPSVLNLAGKKPWEVPHIDTME
                     LWRFGEYKNFVSLNLLAHSLGVPTPKDDIDGSQVGEVYWGQNDLPRIANYCQKDVITV
                     AQVYLRIHGEALVRDENISVK"
     gene            2537..3673
                     /locus_tag="VGQ51_04795"
     CDS             2537..3673
                     /locus_tag="VGQ51_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEV9035910.1"
                     /translation="MKSKLPRVGTTIFTVMSSLAAELGAVNLGQGFPDYPMSDELIAL
                     VAQAMRDGHNQYAHMNGLPRLRQVLAAKIAALYGTTVDPDTQITITPGGTYAIYTALT
                     TILAPGDEVIVFEPAYDSYIPNIGINGAIPVRIDLRLPDYHIDWDEVRQKITPRTRMI
                     LLNTPHNPTGSILRPEDIEQLRAIVKNTDILIHSDEVYEHLVFDGLPHCSILRYPDLL
                     RRSFVSFSFGKVYHCTGWKLGYSVSSPELMSEFRKVHQFNCFSCHTPTQVALAEYMQN
                     AETYLDLAAFMQRKRDHFASLMTQTRFTPIRSYGSFFQLYKYDRISDEPDRDFVLRIT
                     REYGVAAIPVSVFYQEGKDDRVVRFCFAKKEETLEKAAERLIKI"
BASE COUNT          911 a         1008 c          988 g          806 t
ORIGIN      
        1 ggaacaggat gtggtgctga tcctcagcaa aagcggtgaa agcccagaaa taaaaatgct
       61 gctttccctc atccggggtt ttggaaatac cctgatcggg atggtaggga atttgcagtc
      121 tgtattggcc aaacaatcgg atatcgtcct gaatacgacc gtggcccagg aggcctgtac
      181 gaataatctc gctccgacca gcagcacgac cgcccagatg gtcatgggag atgcactggc
      241 ggtcaccctc atggagatgc gtggcttcgg cagcgaagat tttgcccgtt ttcatccggg
      301 cggcacgctg ggcaaacggc tctacctgcg ggttgaggac gtatatactc ataacgaacg
      361 gccccgcgtc gcggtagaca ccatgcttcg ggaggtgatc atcgagatca cctcgaaacg
      421 gctcggtgcg gccgcggtga cggaggaaga cgggacttta ctcggcatca ttaccgatgg
      481 cgatctccgg cggctgctcg aaaaggaggg cgtgagggaa ggactgaagg ccggcgatat
      541 catgaccagg agcccgaaaa cggtggaggc cgatgcgctg gccgtagagg cgctggatct
      601 gatgcgcatc catgacataa cgaggcttgt cgtcaccaat ggtcgccgct acctcggttt
      661 tatcgatctg aacaatctca tccgggaagg aataatataa aagtatgaac agacatcatt
      721 atgtagtcat tatggccggc gggatcggca gccgtttctg gccgatgagc cggcaggatt
      781 atccgaaaca gttcctcgac atcctgaata ccggacggac cctgatccaa tggaccttcg
      841 atcggtttgc cagcttcatt cccgttgaaa atatctatgt ggtcacttcg caggaatatg
      901 gggatatcgt caaggaacag ctgcctcagc tgccggtgag caatatcctc ggcgaaccct
      961 cgcgcaagaa tacggccccc tgtatcgcct acatctcctt caagctacag caatcggacc
     1021 ccaaagcttc gctgatcgta gccccggcgg accatctcat cctcgacaag acggcgttca
     1081 ccaaggtctg tctggaagcc ctcagcttcg tcggcaaaca caatgccttc attaccctgg
     1141 gtatcaaacc cacctacccg aacacgggat acggatatat ccagtatgaa cagcattccg
     1201 tgtcggacaa tgtttacaaa gtgaagacct tcaccgagaa acccaatctc gagctggcga
     1261 agacctttat ggccagcggt gacttcctct ggaatgcggg tatcttcgtc tggcaggtga
     1321 aaaatgtcat ccatgccttc gaaaaatatc tccccgagat gtatgatgtc ttcgccgccg
     1381 aaaaggacaa gttcaatacg ccggccgaaa gatcggcact gcagaccatt tatcctctgt
     1441 gtaccaatat atccatcgac ttcggaatca tggagaaggc ggataatgtt tacgtcatcc
     1501 cgtcttcctt cggctggagc gatctcggta cctggaacag cgcctacgag aatctgaaaa
     1561 aggaccacct ggaaaatgcc gttgccggcg ataatgtcat cgttgtcgac gccagccggt
     1621 gcatggtgca tgccccgaat gacaagctgc tggtggtaca gggtctggat aagtttatcg
     1681 tcgtcgacac atcggacgtg ctcctcatct gccaccgcga taaagaacag gagatcaaag
     1741 aatatgtcgc ggaagtgaag cggaacaagg gagatcagta tctttaggga acaacgctgc
     1801 aaacggcgtc aggtccgccc atgtcaccct atccactgca tacgatcctt ttcctcgata
     1861 tcgaaaccgt ctcccagtat ccggacttca accgcgtacc gggcgaatgg caacagctat
     1921 ggcgtctgaa ggcagaagcg ttgaatcgcg acaagacgcc ggcgaccccc gaggaattgt
     1981 atggaagagc cgccatctat gcggagttcg ggaaggtcat ctgtatcagc tgcggtgtac
     2041 tgtccggacc tccggcggac cggaaacttt ccctgaagtc tttttatggc gatgacgaaa
     2101 aagagctgtt gcagcaattc tacgagctgc tccacaaatg gggctccgaa ggacagaaat
     2161 acctttgcgc acacaacggg aaggactttg actttcccta tctctgccgc cgtatggtca
     2221 tccatggcat gaagatccct tccgtcctca atcttgcggg gaagaaaccc tgggaggtgc
     2281 cgcatatcga tacgatggag ctatggcggt tcggggagta taaaaacttc gtatcgctga
     2341 acctgctggc ccattcgctt ggggtaccta cccccaagga cgatatcgac ggcagccagg
     2401 tgggagaagt ttattgggga cagaatgacc tgccccgtat cgcaaactat tgtcagaagg
     2461 acgttatcac cgtggcacag gtatacctgc gtatccatgg agaggctctc gtccgggatg
     2521 aaaacatcag cgtcaaatga agagtaaatt gcccagggtg ggcacgacga tctttaccgt
     2581 tatgagctcg ctggcggcgg agctgggcgc ggttaatctg ggccagggct tccccgacta
     2641 cccgatgagc gacgagctga tagccctcgt cgctcaggcc atgcgcgatg gccataacca
     2701 atatgcacat atgaacggct tgccgcgtct cagacaagtg ctggcagcga agatcgccgc
     2761 gctttatggg acgaccgtcg accccgacac ccagatcacc atcacgcccg gcggcaccta
     2821 tgccatctat accgcgctga cgaccatcct cgcacccggc gatgaagtca tcgtcttcga
     2881 accggcttac gatagctata ttcccaatat agggatcaat ggcgccatcc cggtacggat
     2941 cgatctgcgt ttacccgatt accacatcga ctgggacgaa gtccgacaaa agatcacgcc
     3001 ccggacgagg atgatccttc tcaatacccc gcacaacccc accggttcta ttctccggcc
     3061 ggaagacata gaacagttgc gggcaatcgt aaagaacacc gatatcctca tccattcgga
     3121 cgaagtgtat gaacaccttg tattcgatgg cctcccgcac tgttcgatcc tgcgttatcc
     3181 ggatctgctc cggaggagct tcgtctcttt ctctttcggc aaggtgtatc actgtacggg
     3241 gtggaaattg ggatacagcg tgtcttcacc cgaactgatg agcgagttcc ggaaggttca
     3301 tcaattcaat tgtttcagct gtcatacgcc gacgcaggtg gctttggctg aatatatgca
     3361 gaatgcggag acctacctgg atctcgccgc cttcatgcaa aggaaacgcg atcattttgc
     3421 aagcctgatg acacagacca ggtttacccc aatacggtct tacggcagtt tttttcaact
     3481 gtacaaatac gaccggatca gcgacgagcc cgacagggat ttcgttttgc ggatcaccag
     3541 ggaatatggc gttgcggcga tacccgtgag cgtattttac caggaaggta aagacgaccg
     3601 tgtggtgcgt ttctgttttg caaaaaagga agagacgctg gaaaaagcag ccgaacggct
     3661 gataaaaatt taaatgccca tgaagcttct tccttacttt tcccattccc tac
//
LOCUS       DASYSV010000155         6733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_31399, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000155 DASYSV010000000
VERSION     DASYSV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31399"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VGQ51_04800"
     CDS             <1..1107
                     /locus_tag="VGQ51_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9035911.1"
                     /translation="FSVSVILIIGSIAVFHQIQYVKDRPVGYDRNSLVMVPMQNGNTR
                     QHYDALRTRLLQTGLIEDVAASQNSVIDTYTTNMGYSWRGKDPSLQEEFTVNTVTPEF
                     GRTTRWKIVEGRDFSSDLATDSNAFIINETAVRYMGFKHPIGEIVDWGANGRYTVIGV
                     VRDMISQNPFESARQMIFNLSRKTDGNNRANLVTIRVKPQVGIHRALAAIGSIFKSYD
                     TDDPFTSSFADQEYAKKFADEERVGQLAGFFTILAILISCLGLLGLSAFVAEQRTREI
                     GIRKVLGASVPHLWSLLSREFLLLVSLSLLIGSPVAYWIMHGWLNSYSYHTGVSWWIF
                     AACALGAIALTLFTVSFQAVRAAIANPVDSLRSE"
     gene            complement(1187..1936)
                     /locus_tag="VGQ51_04805"
     CDS             complement(1187..1936)
                     /locus_tag="VGQ51_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9035912.1"
                     /translation="MEQIKVILVDDERSSLQNLEQKIVEFCPELKIVAAVDQPSEAIF
                     LIRHHRPDVIFLDIEMPHINGFRMLNELGEYESEIVFTTAYNHYAIEALRLSAFDYLM
                     KPVSIQDLRDTVGRLSRHLAAATQERLNVLRQSLSKNISQESKIAVPTWEGLEFILIR
                     NVLRIESSSNYSRIFFISGESLLVTKQLKEFEEMLTPYRFCRVHNVHLINLNYVKKYI
                     RGEGGSVVMENGDEIDVSRRKKDDFLKLINL"
     gene            complement(2099..5176)
                     /locus_tag="VGQ51_04810"
     CDS             complement(2099..5176)
                     /locus_tag="VGQ51_04810"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9035913.1"
                     /translation="MLRTAVLILLLLPGLWARPQPFPQLRFTRLTERDGLSCDKASGV
                     TQDKDGIIWISTNNGLNRWDGYGFTHWFANPDDSMTIPANLIEDLVADKKDNIWMVTS
                     GGVCKFSTLTHKVTRFGHGDTAPAVFRTFDDPRICFVNDEYTYIVSPDGLYQIHSDGH
                     YSVVDEGFSPFVFSERRYTHYYKMVRDREGGLWAFRENHVYRLDPVTKRVRQSFEVGE
                     HVSIYDMLFDSEDHCWVSTWNRGILRIDADGVVSAVPGDFKGVAVERGVEWSYNGRRY
                     LVFATTQPSLVLVDEKTGNTRSYLYEQKMESIGAPFVDRQNMLWVPTTHGVLYVSSSG
                     KLFDFLPVSMGPGGKPDSAYLATAYDLREEPSGYWIARRYNGGMLWFDKDWRLVHGWL
                     KPVDSVGEGFREDIGTIREVYDFRQRGDSMFITTEWGIMVMDLKTLHRTMIRYQGPTP
                     IIRLRTIVPVSDHKWLIRSFSNGVFEFDPVALRFVRHYQLVGQCTGCGNVSANYMVRD
                     HKGRIFLTTNGGLFQYNERADTFLPVRVAGPHSFGNSLIGEAVDSAGLIWLGVDDGIC
                     AYDPDAGRITRVLTEHNSIGPVNRIAIDSLQNVWFSSPAGYWCWLRRQDKLIQFRFSL
                     GLPDNDEGFFYTASNGNVYAGCTGGVIWFHNDRLMQYDATSSVKIMDAVAGGVPVDLT
                     TASNGEKRLTLGPDQNNLQVNFDVINYDLPENNLFYYRLKPGPGSWVQLENGRLSFNN
                     LPAGEYELAVRGGNKVTGNFTAEDRLVFVIRPYWWQSWWFKTLVALALMSSIGVLVWL
                     RIRHIRREAAFRQKMADTELRALRAQMNPHFIFNSLNSIENFMMKNEKWLASDYLNKF
                     ARLFRMILHSSRNELVPFAKDMEALQLYVDLESLRFNNKFDYRTKIDPLLVDGEYRVP
                     SLLIQPYVENAIVHGIGLSKKRGLYVQLTAFLQGDCIHYIIRDNGIGRRQAEEVNQVN
                     RPNHRSVGLSITADRINIFSHQQRSEGSVTITDLWEEDGTAAGTKVEVIIKAV"
     gene            5299..6687
                     /locus_tag="VGQ51_04815"
     CDS             5299..6687
                     /locus_tag="VGQ51_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9035914.1"
                     /translation="MKGYLFTVCLLCYGTLSLGQSTGDLIKQQAGQGVREGAAIATEQ
                     TANNVSNKLINKLFSPKKPKNAKSDSAKTVAALHNGTAAPSSAPSSAAGTPASLSAPS
                     GASAPSASPTTSSDLKTYSKFDFIPGDKILVVEDFAPDAIGDFPDKWNTNSTGEIQTI
                     EGRDGKWLSLTKKGVFLPEFITSLPDNFTLQFDLVCSEKNTVYSGRIGLGFYAIPDPG
                     KQFASLGFSAKRNGVDLSLTPGGGSASGLTRCAVFSDDLQTMSNEIEQTDWVSREPAK
                     RTVRLSIWRQRQRLRMYMNEEKVWDMPRAFETGKNYNSVLFYLDNGTIAGDRYLIGNL
                     CLAVGAPDTRNKLITEGRFSTTGILFDVNSAVLKPESYGSLKDIAEVLKDNGTVNVKI
                     VGHTDSDGDAAQNLALSQKRAAAVKDALIKTFGIDASRLQTDGKGATQPVSPNTSAAG
                     KAQNRRVEFIKL"
BASE COUNT         1555 a         2227 c         1521 g         1430 t
ORIGIN      
        1 ttctccgtat ccgtgatcct catcatcggc agcatcgccg tattccacca gatacaatat
       61 gtgaaggatc ggcccgtcgg ttacgatcgc aacagcctcg tcatggtccc tatgcagaat
      121 ggcaataccc gtcagcacta cgatgccctc cgcacccggt tgctgcaaac cggcctgata
      181 gaagacgtag cggcctcaca aaactccgtc atcgacacct acaccacgaa tatgggctat
      241 agctggcgcg gaaaagaccc gtcgctacag gaagagttca ccgtcaatac cgtgaccccg
      301 gaattcggac gcactacaag atggaagatc gtcgaaggcc gcgatttcag cagcgacctc
      361 gctaccgatt ccaatgcctt catcatcaac gagacggcgg tgagatacat gggcttcaaa
      421 catcccatcg gcgaaatagt cgactgggga gccaacggca ggtataccgt catcggcgtg
      481 gtcagggata tgatcagcca aaaccctttc gaatccgcaa gacagatgat ctttaacctc
      541 agccgcaaaa ccgacggcaa caatcgcgca aacctcgtca ccatccgcgt caaaccgcag
      601 gtcggtatac accgggcgct ggcggccatc gggtccatct ttaaaagcta tgataccgac
      661 gatcccttta cttcatcctt tgccgatcag gaatacgcca aaaaattcgc cgatgaagaa
      721 cgcgtcggcc agctggccgg tttctttacg atcctcgcca tcctcatctc ctgtctcgga
      781 ctcctgggac tctcggcttt cgtagccgaa caacgtaccc gcgagatcgg catccgcaaa
      841 gttctcggcg cctcggtccc ccatctctgg agcctcctgt cccgcgaatt cctgttgctg
      901 gtaagtctgt cgctcctcat cggctcgccc gtggcttact ggatcatgca cggctggctg
      961 aatagctata gctatcacac cggagtgtcc tggtggatct tcgcggcctg cgcgctgggc
     1021 gcaatcgcgc tgaccctgtt caccgtcagc ttccaggcag tgcgcgccgc gatcgccaac
     1081 ccggtcgata gcctgcgttc cgaatagtca ctgctgcgtc cccgccggta tatacccgat
     1141 atccaccaat cccggcgccg tctgcacttc atctgttccg cccccctcac aaattgatca
     1201 acttcaaaaa atcatctttc ttccttctgc tcacatctat ctcatcccca ttctccatca
     1261 ctacactacc gccttcgccg cgtatatact tcttcacgta gtttaagttg atcagatgca
     1321 cgttatgcac gcggcaaaac cggtagggag tcagcatctc ctcgaattcc ttcaattgtt
     1381 tggtcaccag caacgattcg ccgctgataa aaaagatgcg cgaataattc gaactggact
     1441 cgatacgcaa cacattccgg atgaggatga attccagccc ctcccatgtc ggcaccgcga
     1501 tcttgctttc ctggctgata ttcttgctca gcgactgtcg cagcacattc agtctttcct
     1561 gtgtcgccgc ggccagatgc cgggaaagac gacccacggt atcgcgcagg tcctgtatcg
     1621 ataccggctt catcaggtag tcgaaggcac tcagacgcaa cgcctctatg gcatagtggt
     1681 tatatgccgt cgtaaacact atttccgact catactctcc cagctcattc agcatccgga
     1741 aaccattgat atgcggcatc tcgatatcca ggaatataac atccggccgg tgatgcctga
     1801 tcaggaatat ggcttcgctg ggctgatcta cggccgcaac gatcttcagc tctggacaga
     1861 actcgacgat cttctgctcc aggttctgca gactgctacg ctcatcgtct accaatatga
     1921 cttttatctg ctccataata aaggtatgct taacccttcc cactccgccc acctccagct
     1981 cccgccccgg ctcacgcccc gccgacctcc tgcccacctc cggctcccgc cccgccccgc
     2041 cgccttccgc cgccctccag ttgccaccca ccccgcccac cgcgccgccc ccatcaccct
     2101 acaccgcttt aatgatcacc tccaccttcg tccccgccgc cgtcccatcc tcctcccaaa
     2161 gatccgtaat cgtaacactc ccttcagaac gctgctgatg actgaaaata ttgatccggt
     2221 cggccgtgat cgacaatccg acactccggt gattcggccg gttcacctgg ttgacttcct
     2281 ccgcctgtct gcggccaatc ccattgtccc ggatgatata atggatacaa tccccctgca
     2341 ggaaggccgt caactgtaca tacaaccccc tcttcttgct cagcccgatc ccatgcacga
     2401 tcgcattctc gacataaggt tgtatcagca acgacggcac ccggtattct ccgtctacca
     2461 gcaaaggatc gatcttcgta cggtagtcga atttattgtt gaatcgcaag ctctccaggt
     2521 ccacatacag ctgcaaggcc tccatgtcct tggcaaaagg caccagctca ttcctgctgc
     2581 tgtgcaggat catccggaac agccgcgcaa acttattaag atagtcgctc gccagccact
     2641 tctcgttctt catcatgaag ttctcgatgc tattcagact gttgaagata aaatgcgggt
     2701 tcatctgcgc gcgcaacgcc cgcagctcgg tgtccgccat cttttgacga aaagcggctt
     2761 cccgccggat atgccggata cgcagccata ccagcacgcc gatcgacgac atcagcgcca
     2821 gcgctaccaa agtcttgaac caccacgact gccaccaata cggccggatc acgaagacca
     2881 gcctgtcttc tgccgtaaaa ttgcccgtta ccttattgcc gcccctcacc gccagctcat
     2941 attcacccgc gggcaaattg ttgaaagaaa gacgcccgtt ctccagctgt acccaactcc
     3001 ccggcccggg tttgagtcga tagtagaaaa ggttattctc cggcagatcg taattgatga
     3061 catcgaaatt cacctggagg ttattctggt cgggcccaag cgtcaatcgc ttttcaccgt
     3121 tcgaagccgt cgtgaggtcc acgggaacac ccccggcgac cgcatccatg atctttacgc
     3181 tgctggtcgc gtcatactgc attagcctgt cgttatggaa ccatatcacg ccaccggtgc
     3241 accccgcata tacgttgccg ttggaggcag tataaaaaaa gccttcgtca ttgtcgggaa
     3301 gacccaggct gaacctgaac tggatcagct tatcctggcg gcgcagccag caccaatacc
     3361 ccgcgggact gctgaaccag acgttctgga gcgaatcgat ggcgatccgg ttcacggggc
     3421 ctatactgtt gtgctcggtc agcacacgcg ttatgcggcc cgcatcggga tcatacgcac
     3481 aaatgccatc gtcgaccccc aaccagataa gacccgcgct gtccactgcc tcgccgatca
     3541 gcgagttgcc aaaagaatgc ggacccgcca cccgtacggg aaggaaggtg tcagccctct
     3601 cattgtattg gaaaagcccg ccattggtag taagaaagat cctcccttta tgatcccgca
     3661 ccatataatt ggcgctgaca ttgccgcatc ctgtgcattg acccaccagc tgatagtgtc
     3721 gcacaaaacg cagggccacc ggatcgaact cgaaaacacc attgctgaaa ctccggatca
     3781 gccacttatg atcgcttacc ggcacgatcg ttctcagtcg gatgatcggc gttggacctt
     3841 ggtaccggat catagtgcga tgcagcgtct tcagatccat gaccataatc ccccattccg
     3901 tcgtaataaa catgctgtcg cccctctgcc tgaagtcgta cacctccctg atcgtaccga
     3961 tatcctcacg aaaaccctca cctaccgaat ccaccggttt cagccatcca tggaccagtc
     4021 gccagtcctt gtcgaaccaa agcatgcctc cgttatatcg ccgcgcgatc caataaccgc
     4081 tgggttcttc ccgcaagtca taggccgtgg ccaggtacgc actgtccggt tttccccccg
     4141 ggcccatgct caccggcaga aaatcgaaca acttgccgga cgagctgaca tacagaacgc
     4201 catgtgtagt cgggacccag agcatgtttt gacggtcgac gaagggcgcg ccgatacttt
     4261 ccatcttttg ttcgtagaga taagaccgcg tattccccgt cttctcatcg acaaggacga
     4321 gcgaaggttg ggtcgttgca aagaccaggt aacgccggcc attatacgac cattcgaccc
     4381 ctcgctctac cgcgacacct ttgaaatcgc cgggcacggc agatacgacg ccatccgcat
     4441 ctatacgcag aatgcccctg ttccaggtgc ttacccagca atgatcctcg ctgtcgaaaa
     4501 gcatatcgta aatgctgaca tgctccccaa cctcgaaaga ttgccgcacc cgtttggtaa
     4561 caggatcgag acgatagaca tggttctccc ggaaggccca tagcccgccc tctctgtccc
     4621 gcaccatctt ataataatgg gtataccgcc tttccgaaaa tacaaacggt gaaaacccct
     4681 catcgacaac actgtaatgc ccgtcggaat ggatttgata aagcccgtca ggcgagacga
     4741 tgtatgtata ctcatcattg acaaaacaga tcctgggatc atcgaatgtg cgaaagaccg
     4801 ccggagccgt gtctccatgt ccgaacctcg tcaccttatg ggtaagtgtg ctgaatttgc
     4861 agaccccgcc cgacgtcacc atccagatat tatccttctt gtcggcgaca aggtcttcga
     4921 tcagattggc gggtatcgtc atgctgtcgt ccgggttggc gaaccaatgc gtgaaaccgt
     4981 aaccgtccca ccggttcaga ccgttattcg tactgatcca aatgatgcca tccttatcct
     5041 gtgttacgcc gctggccttg tcacaggaaa gcccgtcccg ttcggtcagc cgggtaaacc
     5101 tcagctgtgg aaaaggttgc ggccgggccc atagccccgg tagcagcagc aatatgagta
     5161 cggccgtcct gagcatcgac ctaaattagg ctaaattttg gcgaagtccc taccgaaaat
     5221 gtaatcgggt ccgccggata cgcagtctga tcgccaccac ggtcgccgtc ttcctacttt
     5281 cgccaaaaca ccactattat gaaagggtat ctcttcactg tctgcctcct ctgctacggc
     5341 acattgtccc tcggacagtc caccggtgac cttatcaaac aacaggcagg acaaggcgtc
     5401 cgcgaaggtg cggccatagc caccgaacaa accgccaata atgtttcgaa taaactgata
     5461 aacaaacttt tctccccaaa aaagccaaag aacgccaaat ccgattccgc caaaaccgtc
     5521 gctgccctcc ataacggaac cgccgcaccc tcctccgcac cctcctccgc cgccggtacc
     5581 ccagcctcgc tctccgcacc ctccggcgca tccgcaccct ccgcctcccc caccacctct
     5641 tccgatctca aaacatacag caaattcgat ttcatccccg gcgataagat cctcgtcgtc
     5701 gaagacttcg cccccgacgc gatcggcgac ttccccgata aatggaatac caacagcacc
     5761 ggcgagatac agaccatcga aggtcgtgac ggcaaatggc tgtcgctaac caaaaaaggc
     5821 gtcttcctcc cggaattcat caccagcctc cccgataatt tcacgctcca gttcgacctc
     5881 gtatgcagcg aaaagaatac cgtctattcg ggaaggatcg gtctgggctt ctatgccata
     5941 cccgatcccg gaaaacaatt cgcatccctt ggcttcagtg ccaaacgcaa tggcgtcgat
     6001 ctttccctta cacccggcgg cggatcggcc agcggcctca cccgttgtgc ggtcttcagc
     6061 gatgacctgc agacaatgag caatgagatc gaacagactg actgggtctc ccgcgagccc
     6121 gccaaacgaa ccgtcaggtt gtccatctgg cgccagcgtc agcgtttgcg catgtacatg
     6181 aatgaagaaa aggtctggga catgccccgc gccttcgaga ccggaaagaa ttataacagc
     6241 gttctcttct atctcgacaa cggcaccatc gccggcgacc gctatctcat cggtaacctc
     6301 tgcctggccg tcggcgcccc cgacacccgc aacaagctca ttaccgaagg ccgcttctcc
     6361 actaccggca tcctcttcga cgtcaattcg gcagtcctca aacccgaatc ctatgggtcg
     6421 ctcaaagata tcgccgaagt cctcaaagac aatggcaccg tgaacgtaaa gatcgtcggc
     6481 cataccgaca gcgatggcga cgccgcccaa aatctcgccc tctcgcaaaa acgcgctgcc
     6541 gccgtcaaag acgccctcat caaaacattc ggcatcgacg cctcccgcct gcaaaccgac
     6601 ggcaaaggcg cgacccaacc cgtctccccc aatacctccg ccgccggcaa agcccaaaac
     6661 cgccgcgtcg aattcatcaa actttgatcc atcgcgtgca ggcaggcgca cgcatcccat
     6721 ccggccgctt aga
//
LOCUS       DASYSV010000156         4037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_31631, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000156 DASYSV010000000
VERSION     DASYSV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31631"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VGQ51_04820"
     CDS             complement(<1..923)
                     /locus_tag="VGQ51_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035915.1"
                     /translation="MTRTVLHMILGGLFTLAALAAAAQSSSCPLNSNFSTGTLLHWQA
                     YTGNNSGGNDSNAIKLVYDSSRQFPGGTIGATSLPEYELPSVSGIRVITSQGTDAFGS
                     FDMIPTINGYAYHYSILLGSTSVTNHRNIDSINGQPNPNSGPKTGGYVRGIRYLIDVP
                     AGPSGTPYTLTYAYAMVLENGTHVSDNQPLARAIVSTPAGIIECASPVYFLPTNGGDL
                     DSAISRANGFTPSPIPTPNSTLNPVGQEVCLNDVWTKGWTEVTFDLSPYRGQQISLTF
                     EADNCVPGGHFAYAYFAVRNECAGLQMIGDTL"
     gene            complement(1047..2546)
                     /locus_tag="VGQ51_04825"
     CDS             complement(1047..2546)
                     /locus_tag="VGQ51_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEV9035916.1"
                     /translation="MRTKFIIIAAFIASAAVPGCKKFPADINVSPNSPTTASNAQLLT
                     SAINYIPVVIEGISGALYTQQWAEKPYTDDSRYISVNFDFYGIYAGPLENLQTILGNK
                     SYNITEGSKNNQLAAARILRAWFFWSTTDRWGDIPYSGALKGNAGLAPKYDAQKDIYY
                     DLLNELKQAASQIDDGNAVTGDLLYGGDMGKWKKFANSMRALMALRLSKIDPTRGKTE
                     FADAIAGGVFTDNSDNAVYVHLADPVNQNYWYYVADVQNREWYWISKTLTDYMNPLQD
                     PRLPIFADPAPATGAYAGVPYGLDGDSIAKITTSKVSFMGVHVRAQNAPCFIITYAEL
                     LLAEAEADKTGWLPGGDADAAQKYNAAIEASVRQWVRNSFKAGVIDDVEKAAYDPDDQ
                     GDTTGLAAYLARPEIAYDPANALKQIANQRWVHLYMNGYEAWAEWRRTGYPVLTPAPD
                     NNNIPIPRRQAYPSSEPNINADNYKAAVQAQSGFGGKDDLNGRVWWDKP"
     gene            complement(2559..>4037)
                     /locus_tag="VGQ51_04830"
     CDS             complement(2559..>4037)
                     /locus_tag="VGQ51_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9035917.1"
                     /translation="TQRYDYLQEETAGGFVTPGFYNIANSLERPSVINYLQKKEVRSA
                     YGSVTLGFKNTYFLDASLRNDISSTLPQNSNSYLYPAVSASMVFSQLLKWRTLSFGKA
                     RLSFAQAGADITPYLTTGYYNLGQPYGSNFPMDVPDILNNPNLKPSLGTSYEAGLDLK
                     FFQNRLNASATWYHQNNKNAVLPLDVSGTSGYTTSVINAGNIENHGIELTVGGTPVTT
                     KRFSWISTFNFSHNTSRIISLYPGLHSYQLDYNRYASVNMYLLANEGEPFGTIVGNGY
                     KRDLKTGKVLLDASNLPQYESNHKLGNVLPKFTGGWQNTVTWKNFDLSATIDFQSGGK
                     FLSWTKMLAVKSGQAAETAATNDKGKNVRDDPASGGGVKISGLSSVTGQDVTAYVPAR
                     AYYRNVLGSKIYDPWTEDASYIRLREARLGYTFSRTAFPSFPFRSLNLALIARNPWMI
                     WQKAPKGVNPAELATGASSLNWLETGQLATARSYGVNLTISF"
BASE COUNT          813 a         1071 c         1184 g          969 t
ORIGIN      
        1 agggtgtccc cgatcatctg cagacccgcg cactcattcc ttacggcaaa atacgcatag
       61 gcaaaatgcc cgcccggtac gcaattgtcc gcctcgaagg tcagcgagat ctgttgcccg
      121 cgatacggcg acaggtcaaa ggtcacttcg gtccagccct ttgtccagac atcgttcagg
      181 catacttcct gacccaccgg attgagggtg gagttcgggg tagggatggg gcttggggtg
      241 aacccgtttg cacgcgagat ggccgaatcg aggtctccgc cattggttgg aagaaagtag
      301 acgggggaag cgcattcgat gatccccgcc ggcgtgctga cgatcgcgcg ggccagcggt
      361 tggttatccg agacatgggt gccgttctcc agtaccatgg cataggcata ggtcaacgtg
      421 taaggagtcc ccgacggccc ggccggcacg tcgatcaggt aacgtatccc gcggacatac
      481 ccgccggtct tcgggccgct gttggggttg ggttgtccgt ttatcgagtc gatgttgcga
      541 tggttggtga cggatgtaga gcccagcagg atggaataat ggtaagcata gccgttgatg
      601 gtcgggatca tatcgaagct gccaaacgca tcggttccct ggctggtgat gaccctgatg
      661 ccgcttaccg aaggcagctc gtattcggga agagaggttg caccgatggt gccaccggga
      721 aattggcggc tggaatcata gactaacttg atcgcgttgg aatcattgcc tccgctgtta
      781 ttgcccgtat aggcctgcca gtgcagcagg gtgccggtgg agaaattgct attgagcgga
      841 caggatgatg actgggctgc tgccgccagg gcggcgaggg tgaataagcc ccccaggatc
      901 atatgcaata ccgttcgcgt catagcagca gtttggcccg tctgtaaatt tacttaatta
      961 caaacggaag aaaccaccgg ggggttgaaa aaaaggggcc ggcgaccgcc ggccccggat
     1021 gggtatttca tagtgttaat ccgttgctag ggtttatccc accaaaccct gccgttgagg
     1081 tcgtctttgc ctccaaaacc tgactgtgcc tgcacagcag ccttgtaatt gtcggcattg
     1141 atgttcggtt cgctgctcgg ataggcctgt cttctcggaa tgggaatatt attgttgtcc
     1201 ggcgccggtg tcagcacagg atatccggtg cgtctccatt ccgcccaggc ttcatacccg
     1261 ttcatataca ggtgcaccca tcgctgattg gctatctgtt tcagggcatt ggcgggatcg
     1321 taggctattt ccggccgcgc cagatatgct gcgagtcccg tggtatcccc ctggtcgtcg
     1381 ggatcatagg cggccttttc cacgtcatcg atgacgccgg ccttgaagct attccgtacc
     1441 cattgacgta ccgaggcttc gatggcggca ttgtatttct gcgccgcgtc cgcatcccct
     1501 ccgggcagcc atcctgtctt atccgcctcc gcctctgcca gcaacagctc ggcataggtg
     1561 atgataaaac agggagcatt ctgcgcgcgg acatgtaccc ccatgaaaga gaccttcgag
     1621 gtagtgatct ttgcgatcga atcgccatcc agcccgtaag ggacgcctgc ataggccccc
     1681 gtagcaggcg cggggtcggc gaatatcggc agccggggat cctgcaaagg gttcatgtaa
     1741 tccgtgagcg tcttgctgat ccagtaccac tcacggtttt gtacatccgc tacataatac
     1801 cagtagtttt ggtttacggg gtcggccaga tggacataga cggcattgtc gctgttgtcc
     1861 gtaaaaacgc cgcctgctat cgcatcggca aattcggtct tacccctcgt gggatcgatc
     1921 ttcgagagac gcaacgccat caatgcccgc atggaattgg cgaacttctt ccatttcccc
     1981 atgtccccgc catacaacag atcgcctgtc acggcattcc catcgtctat ctgcgaggcg
     2041 gcctgtttca gctcgttcag cagatcgtaa tagatatctt tttgcgcatc gtatttgggt
     2101 gcgagaccgg cattgccctt caacgctccc gaataaggga tatctcccca ccggtccgtc
     2161 gtcgaccaga agaaccaggc ccgcaggata cgggctgccg ccagctggtt gttcttcgac
     2221 ccttcggtga tattgtagct tttgttgccc agtatggttt gaaggttttc cagcggaccg
     2281 gcatagatgc catagaaatc gaagttgacg gaaatatacc gggaatcgtc agtatagggt
     2341 ttttcggccc attgctgcgt gtacaatgcc ccggagatcc cttcgataac gacaggtatg
     2401 tagttgatcg cggaagtgag taattgcgca ttgctggcgg tggtggggct gttgggactg
     2461 acattgatgt cggcagggaa ttttttacat cccggtacag cggccgacgc tataaatgct
     2521 gctatgatga tgaattttgt tcgcatggtg gatgattttt aaaaggagat ggtcaggttt
     2581 acgccatagg agcgggccgt ggccagctgc cccgtttcca gccagttgag cgaggatgca
     2641 ccggtagcca gctcagccgg attgacgcct ttgggcgcct tctgccagat catccaggga
     2701 ttgcgcgcta tgagcgccag gttcaggctc ctgaacggaa acgacgggaa ggccgtcctt
     2761 gagaacgtat aacccagtct tgcctcgcgc agccggatat agctggcgtc ctccgtccat
     2821 ggatcataga tcttggagcc caggacattc cggtaataag cccttgccgg cacataggcg
     2881 gtgacgtcct ggcccgtgac cgaagagagc cccgatattt taacgcctcc gccggaggcc
     2941 ggatcgtccc tgacattctt gcctttatcg ttggtggccg cagtttcggc cgcctgtccg
     3001 ctctttaccg ccagcatctt cgtccagctg aggaatttgc cgccgctttg gaaatcgatg
     3061 gttgcggaca ggtcgaaatt cttccaggtc accgtgttct gccatccccc ggtaaacttt
     3121 ggtaagacat tacccaattt gtgattcgac tcatattgcg gcaggttgct cgcgtcgagg
     3181 aggaccttgc ctgttttcag atcccgctta tagccgttgc ccacgatggt gccgaagggt
     3241 tcaccttcat tcgccagcag gtacatattg acgctggcat atcggttgta gtccagctga
     3301 taggagtgaa gaccgggata cagcgagatg atcctgctcg tgttgtgact gaaattgaag
     3361 gtgctgatcc agctgaagcg tttagtcgtc accggtgtac cgccgacggt cagctcgatg
     3421 ccatggttct ctatgtttcc cgcattgatg acggaggtcg tatagccgct ggtgcccgat
     3481 acatccaggg ggagcaccgc gttcttatta ttctgatggt accaggtggc gctggcattg
     3541 agccggtttt ggaagaactt caggtccaga ccggcctcat acgaggtgcc cagcgaaggt
     3601 ttcagattcg gattgttgag gatgtcggga acgtccatgg gaaaattgct gccatagggc
     3661 tgacccagat tgtagtaccc ggtagtgagg taaggcgtga tgtccgcgcc ggcctgtgcg
     3721 aaactcagac gggccttgcc gaatgacaac gtccgccatt tcagcagctg gctgaagacc
     3781 atgctggcgg acacggcggg atacaggtag gaattgctgt tctgaggcag cgtggaagaa
     3841 atgtcgtttc ggagacttgc atccaggaaa taagtgttct tgaatccgag cgttaccgag
     3901 ccgtaggcgc tcctgacctc ttttttctgc aggtagttga tcacggaggg tctttccagc
     3961 gagttggcga tattatagaa tcccggtgtg acaaagccgc ctgcagtttc ctcctgcagg
     4021 tagtcgtacc gttgggt
//
LOCUS       DASYSV010000157         6176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1053893, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000157 DASYSV010000000
VERSION     DASYSV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053893"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VGQ51_04835"
     CDS             complement(<1..855)
                     /locus_tag="VGQ51_04835"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEV9035918.1"
                     /translation="MSNTPIIGITMGDPASIGPEIAVKALLTDAVRSICRPVIIGDAA
                     TLRSIIDQLRWKASVHAIESVHEARFEPGVIDVLDMKTVRLDKLVLGEISAMAGHAAF
                     EAVRKVIALAMAGEIDATVTGPINKKSINEAGHHFAGHTEIYAHYTDTKKYGMLLVED
                     NLKVIHVSTHVSLRHACDLVKKDRVLAVIGLLRDGLISLGETNLKIGVAGLNPHAGDS
                     GLFGTEDDQEILPAVLEARRQGYDVDGPVPPDTLFAKAVTGAYGGVVAMYHDQGHIPF
                     KLTGFQWNA"
     gene            complement(863..1687)
                     /locus_tag="VGQ51_04840"
     CDS             complement(863..1687)
                     /locus_tag="VGQ51_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV9035919.1"
                     /translation="MNKKYRGIIVPAITPLTADRTLDTGAAERLLTSFPHPFILGTTG
                     EAPSLPAELKKQYIRLAGKVKKTGQQLYVGISSNCPDESVEWARFAFGEGADVVVSNL
                     PSYYPLTDDLIRRHFEWLIARVNGPLVIYNIPSTTHHSIPLAIIDELSHHPNVVATKD
                     SERNEERLHASLKLWAGREDFSHFLGWAARSGEALLNGCDGLVPSNGNLDAGVYVDLE
                     KAAAKGDKTEVQRLQAISDQLGASYQSGRSLGESLAALKSLMHQRGLCQPYMMPPL"
     gene            complement(1680..2816)
                     /locus_tag="VGQ51_04845"
     CDS             complement(1680..2816)
                     /locus_tag="VGQ51_04845"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV9035920.1"
                     /translation="MSTTPPLRIFFPGRLVFGNGCLDQLSDELIRLSPSRILVITIDP
                     LLSSLSGLFTLLEANGISVTVYTRIVQEPSFEDFHDVMREAAPLCPDIVLGIGGGSVL
                     DIAKLVAAQLDNDQQLADYVGNGLLKGRKRTLVCMPATSGTGSEVSPNAILVDNADNQ
                     KKGVISPFLVADIVYVDPLLTRSVPPAVTAATGLDALTHCLEAYTNKFAQPFIDVYAF
                     EGMRLIAENLVRAVRNGADEEARSQVAMGSLLGGFCLGPVNTAAVHALSYPLGSLFHL
                     PHGLSNALLLPYVMEFNIPAAPERYANVARALGCGTAEGGVEKIRSLIRECGLPATLK
                     EVGVPLDAIDGMAADAMKITRLLNNNPRPVTLEDAINIYIAAYE"
     gene            complement(2829..3806)
                     /locus_tag="VGQ51_04850"
     CDS             complement(2829..3806)
                     /locus_tag="VGQ51_04850"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV9035921.1"
                     /translation="MEEKKFVPVMITPFNLKAKVDLDAVAVLVEFYLEAGVKGFFANC
                     LSSEMYSISEDERLELTRHVVECVDGRVPIVATGSFGLTIEDKADFTARIYDTGIDAV
                     ILITGHFANVEDPDAILLRNFSKMLRLTRDIPLGLYECPAPYKRIIPPDVFKHLLDTG
                     RLVYHKDTSIDLEKVKAKLEVLKTGQYDLEFYDAHTPNAMYSLQMGAKGMSSISGNFY
                     PEILVWMCDHANDPDRQEDVRWLQGELSRVDPLIHVAYPMSAKYFLQKRGLPVRTISR
                     AHALELTVEQKRTLDDIHQSFLGWCERLDITPVQTGRFIRPKLPLDPPI"
     gene            complement(3812..4891)
                     /locus_tag="VGQ51_04855"
     CDS             complement(3812..4891)
                     /locus_tag="VGQ51_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEV9035922.1"
                     /translation="MYRLTSIGFLLMLTLHSNGQPIVRSEFIYDTAPFPECHAATIAE
                     TPKGLVTAFFGGTKERNPDVCIYVSRKDKGTHRWTTPVNVANGIQNEKLRYPCWNPVL
                     YQAPGGDLLLFYKVGPSPSTWKGWLVRSRDGGVSWGKPEALPEGFLGPIKNQPVRLSD
                     GTLLCPTSVEGHGWKVHFELTQDNAKTWQMAAPVPASDSLRAIQPTILLRKDGSIEAL
                     CRSENRAILSTVSKDKGRSWSPLEPTALPNNNSGIDGVTLADGRQVLVYNHVLPPPGA
                     TKGERSPLNVAVSTDGHTWYAALVLEDDHDGQYSYPTVIQSKDGHVHIVYTWRRKKIK
                     YVEIDPSKLKLIKIENGVWPSSVKK"
     gene            complement(4896..5549)
                     /locus_tag="VGQ51_04860"
     CDS             complement(4896..5549)
                     /locus_tag="VGQ51_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3826 domain-containing protein"
                     /protein_id="HEV9035923.1"
                     /translation="MKKISLFLMMTCTLAAGAQTPSIDSAITKKAASWVASLNLNDAA
                     KETRLTAVIATHLNTVRNWNNDHPYTEVPAGIDPATGRPLSKMDRQIIAVSAIPKTTH
                     DSLLDGLRKDLTPDQVNAILDKYTMGKVQFTFNGYKAIVPDLTPKEDSVILSNLEQAR
                     EQAVDYKNSKEISAIFEIYKTKNEQYLNANGRNWHALFKAYVDAQKAKKAAASKQNK"
     gene            complement(5546..>6176)
                     /locus_tag="VGQ51_04865"
     CDS             complement(5546..>6176)
                     /locus_tag="VGQ51_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV9035924.1"
                     /translation="DDGYGNNSVTSNLLPLYFGMVPQRARAAVASRLVERVASDGHIA
                     TGVIGTGWLMRGLTDLGRSDLALRLATNKDYPGWGYMVGQGATTIWELWNGNTADPAM
                     NSQNHVMLLGDLLTWCFRDLAGIAPADPGYKKIRLQPVFPEKLDSVTASYNTPYGIVT
                     SEWRREGGHISWQITVPPNTRAIVHLPDGSESETGSGEYHYDIQTKKSI"
BASE COUNT         1325 a         1752 c         1681 g         1418 t
ORIGIN      
        1 cgcattccat tggaatccgg ttaatttgaa agggatatgt ccctgatcgt gatacatcgc
       61 gacaacgccg ccataggccc cggtcaccgc cttggcgaac aaggtatccg ggggtacggg
      121 gccatccacg tcataaccct gccggcgtgc ttcgagcacg gcgggaagta tctcctggtc
      181 gtcttcggtg ccgaaaagac ccgaatcacc cgcatgcgga ttgagacccg ccacgccgat
      241 cttcagattt gtctcgccca ggctgataag gccatcgcgt aagagaccga taacagccag
      301 tacgcggtcc ttctttacca ggtcacaggc gtgccgcaaa gaaacatgtg tggaaacatg
      361 aatgaccttc agattatcct cgacgagtaa catcccgtat ttcttcgtgt cggtgtaatg
      421 cgcatagatc tccgtatgac cggcgaagtg atgacctgct tcattgatcg atttcttatt
      481 gatcggaccc gtgaccgtgg catcgatctc gccggccatc gcgagcgcga tcactttccg
      541 aacggcttcg aaggcggcgt gacccgccat ggccgatatt tccccaagga ccagcttatc
      601 caacctgacc gtcttcatat cgaggacatc gatgactccc ggttcgaacc gggcttcatg
      661 aacgctctcg atggcatgga cggaggcctt ccatctcagc tgatcgatga tggaacgaag
      721 ggtagcggca tcgccgatga tcacggggcg gcagatagac cggaccgcgt cggtgagcag
      781 ggccttgacg gcgatctccg gtccgatact ggccgggtcg cccatggtaa ttcctatgat
      841 cggtgtgttt gacatcctaa agctataggg gcggcatcat atagggttga caaagaccac
      901 gctggtgcat caaagatttc aacgcagcca gcgattcgcc caggctacgg ccgctctgat
      961 acgaggcgcc cagctggtcc gaaatggctt gcagccgctg gacttccgtc ttgtctcctt
     1021 tcgcagctgc cttttccaga tcgacatata cgccggcgtc caggttgcca ttgctgggaa
     1081 ccagcccgtc gcagccattc agcagggcct cacccgacct cgccgcccaa ccgagaaaat
     1141 gactgaaatc ttcacgaccc gcccatagtt tcaatgaagc atgcaatcgt tcttcattac
     1201 gttcggaatc cttggtcgct acgacgttcg gatgatggct cagctcgtcg atgatggcca
     1261 gcggtatcga atgatgcgtg gtcgacggga tattgtaaat gaccagggga ccgttcacac
     1321 gtgcgatcaa ccattcgaaa tgccgccgga tcaggtcgtc ggtcagcggg taatacgacg
     1381 ggaggttgct taccaccacg tcggcgccct cgccaaaagc gaaacgcgcc cattcgacgg
     1441 actcatcggg gcaattcgac gatatgccca cgtacaactg ctgaccggtc ttcttgacct
     1501 ttcccgccag acggatatac tgtttcttga gctcggccgg cagcgatggc gcctcaccgg
     1561 tggtcccgag gatgaaagga tgcggaaagg acgtcaacag tctttccgcg gcgccggtat
     1621 ccagcgtacg atccgccgtc agcggtgtaa tggcgggaac aatgatcccg cgatattttt
     1681 tattcatatg cagcaatata aatattaatg gcatcttcca gcgtgacggg ccgcggattg
     1741 ttattcaaaa gccgggtgat cttcatcgcg tccgccgcca tcccatcgat tgcatcgagc
     1801 ggcacaccca cctccttcag cgtcgccggc aatccgcatt cgcggatcag gctccgtatc
     1861 ttctccactc ccccctccgc ggtcccacac ccgagggcgc gagccacgtt cgcataccgc
     1921 tccggcgccg ccggaatatt gaattccatg acatagggca acagcaacgc attggataac
     1981 ccatgcggca ggtgaaaaag gctgcccagc gggtaagaaa gcgcatgcac ggcggcagta
     2041 ttcacaggac ccaaacagaa ccctcccaac aggctaccca tagccacctg cgagcgggct
     2101 tcttcatcgg cgccattccg caccgcacgg acgagatttt ctgcgatcag ccgcatgcct
     2161 tcgaaggcgt agacgtcgat aaaaggctga gcgaatttat ttgtataagc ctccaggcaa
     2221 tgggtcagtg catccagccc ggtggcggcg gtgacggccg gcggtacgct acgcgtcagc
     2281 agcgggtcta cgtacacgat gtccgcgacg aggaaaggac tgatgacccc ctttttttga
     2341 ttgtccgcat tgtctacaag gatggcattg ggagaaacct cgctgcctgt gcccgaggtg
     2401 gccggcatac agaccagcgt tcgctttctt cctttcagca agccattgcc tacatagtca
     2461 gccagctgct gatcgttgtc cagctgcgcg gcaaccagtt tggcgatatc gagcacgctg
     2521 ccacccccga tgcccagcac gatgtcggga catagtggcg cggcttcgcg catcacatcg
     2581 tgaaaatctt cgaatgaagg ttcctgtacg atacgtgtat aaacagtgac actgatgccg
     2641 ttggcttcca acagggtgaa aagtccggac agcgaggata acaatggatc tatcgtgatc
     2701 accagtatac gggaaggtga cagccggatc aattcatcgg acagctggtc cagacagccg
     2761 ttgccgaaaa cgagccggcc agggaaaaag atgcgtagtg gtggtgtagt cgacatggtc
     2821 gtcaatcatt agattggggg atcgagtggc aatttcggcc ggataaaccg gcctgtctgt
     2881 acgggcgtta tatccagtct ttcgcaccac ccgagaaaac tctgatggat gtcgtcgagc
     2941 gtgcgtttct gctctaccgt cagctcgagc gcgtgcgcgc ggctgatggt ccttaccggc
     3001 aatcctcttt tctgaaggaa atacttggcg ctcatcggat aggcgacatg gatcagcggg
     3061 tcgacacggc taagctcgcc ctgcagccag cggacatcct cctggcgatc gggatcatta
     3121 gcgtgatcac acatccagac gaggatctcc gggtagaaat tacccgagat cgaggacatg
     3181 cctttggcac ccatttgcag cgaatacatg gcattgggag tatgggcatc ataaaattcc
     3241 aggtcatact ggcccgtttt cagcacctcc agcttcgcct tcactttttc gagatcgatg
     3301 gatgtatcct tatgatagac cagtctcccg gtatcgagca ggtgtttgaa aacgtcagga
     3361 ggtatgatcc gtttgtaggg cgcgggacat tcataaagcc cgagcgggat gtcgcgggtc
     3421 agacgtaaca tcttgctgaa attccggagc aagatggcgt cagggtcctc cacattggca
     3481 aaatggccgg tgatcaggat gacggcgtcg atgcccgtgt catagatgcg cgcggtgaaa
     3541 tccgccttgt cttcgatggt cagcccgaac gaacctgtgg cgacgatggg aacgcgtcca
     3601 tccacacatt ccactacatg acgcgtcagc tcgagacgct cgtcctcgct gatgctgtac
     3661 atttcgctgc tgagacagtt ggcaaaaaaa cccttgacgc ccgcctcgag atagaactcc
     3721 accagcacgg ctacggcgtc cagatccacc ttcgccttca gattgaaggg agtgatcatc
     3781 accggaacaa atttcttctc ctccatatgc attatttttt aaccgaggac ggccaaacgc
     3841 cattctctat tttgatcaat ttcaatttcg aaggatcgat ctccacatat tttatcttct
     3901 ttcgtcgcca ggtatatacg atatgtacat gcccgtcttt cgattggatc acggtcggat
     3961 aggaatattg gccatcatga tcgtcttcca gtacaagcgc ggcataccag gtatgcccgt
     4021 ccgtagatac cgccacgttc agcggcgaac gctcaccttt ggtggcgccc ggcggcggaa
     4081 ggacatgatt gtacacgagt acctgtctgc catcggcaag tgtcacgccg tcgatgccgg
     4141 agttgttatt gggtagcgct gtcggctcca gcggcgacca gctgcggcct ttatctttgg
     4201 aaacagtcga aagtatcgca cggttctcgc ttcggcacaa tgcctcgata ctgccatcct
     4261 tccgcagcag gatggtgggc tggatggccc tcagggaatc ggatgcaggg acgggcgcgg
     4321 ccatttgcca ggtcttcgca ttatcctgcg tcagctcgaa atgtactttc cacccatgcc
     4381 cttcgacgct ggtgggacaa agcagcgttc cgtcactgag ccgtacaggc tggtttttga
     4441 tcggaccgag aaacccttcg ggcaaagctt ccggtttccc ccagctgacg ccaccatcgc
     4501 gactcctgac caaccatcct ttccacgtgg atgggctggg gcccacttta tagaacagca
     4561 gcaggtcgcc ccccggcgcc tggtacagca cgggattcca gcagggatag cgcagctttt
     4621 cattctggat cccattggcg acattgaccg gtgtcgtcca tcggtgagtc cctttatcct
     4681 tccggctgac ataaatgcaa acatccgggt tccgctcctt cgtccctcca aaaaaggccg
     4741 ttacgagccc ctttggcgtc tcggcgatgg tcgccgcatg acattcgggg aagggggcgg
     4801 tatcataaat gaattcactt cggacgatcg gctggccgtt ggaatgaagg gtcagcatca
     4861 ataaaaatcc tatggaggtc agtctgtaca tatacttatt tattctgttt ggaagccgcc
     4921 gcttttttcg ccttttgtgc atctacatag gctttgaata aagcatgcca gttgcggccg
     4981 ttggcattca ggtattgctc gttctttgtc ttatagatct cgaagatggc ggatatctcc
     5041 ttgctgttct tataatcgac ggcctgctcg cgcgcctgtt cgaggttaga tagtatgacg
     5101 gagtcttcct tcggcgtaag atcgggcact atcgctttat agccgttaaa ggtgaattgg
     5161 acctttccca tggtgtattt gtcaaggatg gcattcacct ggtccggtgt gaggtctttg
     5221 cgcagcccgt cgagaaggct gtcatgcgtc gtctttggta tcgccgagac ggcaatgatc
     5281 tgacggtcca ttttggacag cggtcttccg gtggcggggt cgatgccggc agggacttcc
     5341 gtgtagggat ggtcgttatt ccagttcctg accgtattga gatgtgtggc gatgacggcg
     5401 gtcagacgcg tctcttttgc cgcgtcgttc aaattcagtg aggccaccca tgaggcagcc
     5461 tttttggtga ttgcactatc gatcgaaggg gtctgggcgc cggcggccag cgtacatgtc
     5521 atcatcagaa aaagagaaat ttttttcata tcgacttttt tgtttgaatg tcataatgat
     5581 attctcccga gccggtctcg ctttcgctgc cgtcggggag atgcacgatg gcccgggtat
     5641 tgggcggcac agtgatctgc catgagatat gcccgccctc gcgccgccat tcgctggtca
     5701 cgatcccgta aggcgtattg taagaggccg tgacactgtc gagtttctcc gggaagacgg
     5761 gctgcaaccg gatctttttg tacccgggat cagcaggcgc aatacctgcc aggtcccgga
     5821 agcaccaggt cagcagatcg ccaagcagca tgacgtggtt ctgtgaattc atggccgggt
     5881 cggcagtatt tccattccat agctcccata tcgtggtggc gccttgtccg accatataac
     5941 cccagcccgg ataatcctta ttcgtagcca accggagcgc cagatcggat ctccccagat
     6001 cagttagccc gcgcatcagc catccggttc caataacccc ggtagcaata tgcccatccg
     6061 aagccacccg ttcaactaac ctggaagcga cggctgcacg cgctcgctgc ggcaccatcc
     6121 cgaaatacag cggcagcaaa ttcgacgtca cgctattatt cccatatccg tcatca
//
LOCUS       DASYSV010000158         8750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2336280, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000158 DASYSV010000000
VERSION     DASYSV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2336280"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VGQ51_04870"
     CDS             <1..634
                     /locus_tag="VGQ51_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV9035925.1"
                     /translation="GVTSLKPEGGPDGAKIGEVSAGSAAEKAGLKQGDLITKVDDITI
                     DGPESLADAIRGYKPEDKVTITYKRDGKEQKATAVLGKSKEATVWNMAPEPYISRDFK
                     KMMPQGSYNFSYKLDNSPRLGIHAQDTEDGKGVKILEVDEESNAAKAGLKEGDIITKF
                     EGKEVNSAMELAQIARESRTKPSVHVDLLRGGKSMSLDVKIPRKLKTADL"
     gene            698..2149
                     /gene="gatB"
                     /locus_tag="VGQ51_04875"
     CDS             698..2149
                     /gene="gatB"
                     /locus_tag="VGQ51_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV9035926.1"
                     /translation="MGINYDAYEIVVGLEVHAQLLTRTKLFCGDSAVFGAEPNTQISP
                     VTLALPGALPRLNKLAVEYAIRMGLACHSEIERNNYFARKNYFYPDLPKGYQISQHTT
                     PICKGGRVSIRTAAGERVICLNRIHLEEDAGKSLHDVAPDTTSIDLNRAGVPLIEIVT
                     EPDIRSGDEAYAYLTELRQLLRYLEICDGNMEEGSMRCDANVSVRLKGETALGTKVEV
                     KNLNSIRNVRRAIEFEARRLIGLLAAGEPIQQQTRSFDASEGTTFPLRTKEEANDYRY
                     FADPDLPPFVVTDELLERVRASLPALPEELVQRYTSDLQLPEYDARVIVSDKDSAVYF
                     GRLIAHTTNYKAAANWMLGPVRSGLNELSLEWKDLPVPPDALAELIRLVEEGRLSFSI
                     AAQRIYPVLGLEPSRKPLDIAIALNLLQSSDTTEIAVWIEQALAGMPDKVMEYKKGKK
                     GLIGLFVGEVKKLSKGKADPRLTNQLLLEKLEN"
     gene            2153..3349
                     /locus_tag="VGQ51_04880"
     CDS             2153..3349
                     /locus_tag="VGQ51_04880"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV9035927.1"
                     /translation="MTKHLSRIVPVVMMACVLFTLSCQPKKKGTFTVEGTFKNADKLS
                     AMAGPISKVYLEEVSYGKDQAPVILDSARLPVTSGSFTVTGAVKTQEIFEVVFGSNVI
                     AVPLINDASEIRVNVDLGKQDDFYTVSGSEASGQLKDLITAFGKKNFEVEKSMADLDS
                     LKQANAADTQLLAATGVKNNAIQDLNIYLKQVVNTNTNATICALALSWASRSFPRSDF
                     EASLSDLVRKYPENGVLTALRQSYDLQKEQLVQQEAHDQANSWVGKQAPDLSLPDANG
                     NTVSLSSFKGKYLLVDFWASWCGPCRAENPNVVIAYNQFKGKNFTIVGVSLDKEKDAW
                     QEAIRADKLDWTQVSDLKFWESKAVKTFNFDGIPFNVLIDPQGKIIAQGLRGDDLEKE
                     LKSVLK"
     gene            3395..4549
                     /locus_tag="VGQ51_04885"
     CDS             3395..4549
                     /locus_tag="VGQ51_04885"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV9035928.1"
                     /translation="MGLIRLLLALAVVATHCGPIFGCNLVGGQIAVQSFFIISGFYMS
                     VILNEKYVGVNNSYRLFITNRFMRLYPIYWAVFVATMLVCIGISYLNADHTAPVFVNY
                     TSFKTSWFASTFLILINLLIFGQGLAMFLGLNPDGNLFLTANFRNTNPYVYTFLFIPQ
                     AWTLGLELTFYLIAPFILRRGLKTVVPLIIGAFLLRMVLYDRFHLQNDPWTYRFFPTE
                     VMFFLLGYVSYRIYLKLKTKHIPPSYCRALLIFTVLFTILYTHLPTLKFGFLPFSMRD
                     FAYFTTMLIAIPLLFNFLKKNRLDSKIGELSYPIYISHMLVLMICDVFSERLSSGFLK
                     ASWVVALLTIAVSYGLNIAIAAPIEKYRQSRLTKTRLQPAMQTPIQLRLF"
     gene            complement(4611..4682)
                     /locus_tag="VGQ51_04890"
     tRNA            complement(4611..4682)
                     /locus_tag="VGQ51_04890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4647..4649),aa:Arg,seq:ccg)
     gene            complement(4756..5376)
                     /gene="cobC"
                     /locus_tag="VGQ51_04895"
     CDS             complement(4756..5376)
                     /gene="cobC"
                     /locus_tag="VGQ51_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HEV9035929.1"
                     /translation="MKEIYLIRHTTPSVEKGICYGQTDLDVTESFREEAEMILKSLPA
                     GIMTVYSSPLKRCTLLAEHLFPGVPVSLRRELMELHCGSWEMQPWDQLPKEEIDPWMA
                     DFVNIRVPGGESYLDLHERVMNCFTAIAATGGGPQVIVAHGGVLRSILAGITGTPLID
                     SFKAFALHYGCVVRVRPEEGAFEMLWNRTPPEKEQHKPSSFYKRFV"
     gene            complement(5373..6137)
                     /locus_tag="VGQ51_04900"
     CDS             complement(5373..6137)
                     /locus_tag="VGQ51_04900"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEV9035930.1"
                     /translation="MKKEVRIFFTAVMFLTRLRVPSSVDHSPGYMRQAPRYFPVIGWI
                     VGAISSLFYFLFARYVSVEIGIAASMIAGVLVTGAFHEDGFADVCDGFGGGWSKEKIL
                     LIMKDSRIGAFGAIGLIVILGTKFLVLKELTPGPFFIVALIAAHSLSRLMPVLVMQTS
                     RYAGDTDTSKSGSMTSQRIGTGGLVTAVVLGLAPFALLPPGFLLAVAPALIATWLMYR
                     FFYRWIGGYTGDCLGAIQQVSEITIYLGFVVIWRYL"
     gene            complement(6140..7186)
                     /gene="cobT"
                     /locus_tag="VGQ51_04905"
     CDS             complement(6140..7186)
                     /gene="cobT"
                     /locus_tag="VGQ51_04905"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEV9035931.1"
                     /translation="MQTGTQATLRQRLQHVIDNKTKPLGALGRLEEIALQTGLIQGTL
                     HPQLNRPYIVVFAGDHGIAATGTVNPYPQAVTAQMVLNFITGGAAINVLCRLHHIGLC
                     VVDAGVNFDFDAAIFPGKLISKKIGHGTRNYLETAAMTPEEAERAVEAGKEVVTDLYA
                     SGCNCVGFGEMGIGNTSSASLIMSYVTGIPVEECVGRGTGTNDEQLTKKKEVLRDAFR
                     RHLPAVAAHPGAMTILSHVGGFEIAMMTGAYLKAAELRMVILVDGFISTAALLLARLL
                     RSDVQDCCFFAHASGESGHARMLEYLGADPLLDLGMRLGEGTGAAMAFPLLQSAVHFM
                     REMASFESAGVSKA"
     gene            complement(7191..7703)
                     /locus_tag="VGQ51_04910"
     CDS             complement(7191..7703)
                     /locus_tag="VGQ51_04910"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEV9035932.1"
                     /translation="MMIFITGGARSGKSRYAQERALQFSGNPVYVATARIWDEEFRNR
                     VDRHRQDRDERWTLLEEEKYIGGLSLDGKTAVIDCVTLWINNFYSDLRYDAEACLEVC
                     RQEVDRLLQQEARIIIISNEIGMGMHADTEAGRKFTDLQGWVNQYIAGRADEVIFMIS
                     GIPLTVKQNK"
     gene            complement(7700..>8750)
                     /locus_tag="VGQ51_04915"
     CDS             complement(7700..>8750)
                     /locus_tag="VGQ51_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9035933.1"
                     /translation="RNYLDDSAMAIAQGGSFSQSSYTGRSHFAELYTNLALSRHVDLL
                     AGIDYRNQGTDQSNVYLSQYYSDTSKIGFDSARVRQSAGYVSLQIKELGGFNLGLGGR
                     YNGFSKYGNVVTYSIDPSYVIDDRVKLFANLSSGFSAPTLYQLYSPYSDPYGQLSPEK
                     TESLEMGAQYSRKHFNARGVFFQRHTKDEIIFTYDATYYNGFYINQDRQDDYGMEWEV
                     SWKAGPWSLTGNYTYITGKGTTLSGGKDTSFYNLYRQPKNLLNLKAGWQPDEGLYISL
                     SLRTVGKRIEPVYGSAPIPVASYYTLNGYAEYRLAKTWKFFLDLENLTDRQYFDIPGF
                     NSKRFNFMAGIAVHL"
BASE COUNT         2125 a         2428 c         2222 g         1975 t
ORIGIN      
        1 gggtgtcact tcgctcaaac cggaaggcgg tcccgacggc gcgaaaatag gagaagtgtc
       61 ggccggtagc gccgccgaaa aagcgggact gaaacaaggc gatctcatca cgaaggtgga
      121 cgatattacg atcgacggtc ccgaatccct cgcagatgct atccgcgggt ataaacctga
      181 ggacaaggtc accataacgt ataaaaggga tggaaaagaa caaaaggcga cagccgtgct
      241 cggcaagagc aaagaagcga ccgtctggaa tatggccccc gaaccgtaca tctcccgtga
      301 tttcaaaaag atgatgccgc agggaagtta taactttagc tacaaactcg acaacagccc
      361 gcgtctcggc atccatgcgc aggataccga agatggcaaa ggggtgaaga tattggaagt
      421 ggatgaagag tcgaacgctg cgaaagcagg gctaaaggaa ggggacatca tcacgaagtt
      481 tgaggggaaa gaggtcaata gcgccatgga actggcccag atagcaaggg aaagtcgcac
      541 caaacccagc gtccacgtcg atctgctgcg gggaggcaaa tccatgagcc tggatgtaaa
      601 gataccgagg aagctgaaga ccgccgatct ctgatatagc ccccactttt ttcgtagctt
      661 tgctggctgc cggttcgccg acagccagtt ttattatatg gggattaatt acgatgcata
      721 cgaaatagtg gtggggctcg aagtgcatgc acagctgttg acccggacca agctattttg
      781 cggcgacagc gcggtgtttg gagcagaacc caatacacag ataagtcccg tcacgctggc
      841 tttacccggc gcgctaccgc gtctcaacaa gctggcggtc gaatatgcga tccgcatggg
      901 tctcgcctgt catagcgaga tcgagcggaa caattatttt gcccgaaaga actatttcta
      961 tcccgacctt cccaagggtt accagatatc gcaacatacg acgcccatat gcaaaggcgg
     1021 aagggtgagc atccggaccg cggcgggcga gcgcgtgatc tgcctcaacc gcatccatct
     1081 tgaagaagat gccggcaaaa gcctccacga tgtggccccc gataccactt ccatcgatct
     1141 gaacagggcg ggggtgccgt tgatcgagat cgtcaccgaa ccggatatac gcagcggcga
     1201 cgaagcctat gcgtatctta ccgagctccg ccagttgtta cgctacctgg aaatttgtga
     1261 cgggaatatg gaggaaggca gcatgcgttg tgatgccaat gtctccgtca ggcttaaagg
     1321 cgagacagcg ctgggtacga aggtcgaggt caagaatctc aactctatcc gcaacgtccg
     1381 gcgggcaata gaattcgaag cccgcaggct catcggtctg ctggcggccg gagaacccat
     1441 tcaacagcag acccgcagtt tcgatgcgtc ggaaggaacg accttcccgc tgaggacgaa
     1501 agaagaagcc aacgattacc ggtattttgc ggatccggat cttccgccgt ttgtggtgac
     1561 agacgaatta ttggagcgtg tgcgcgcgtc gctcccggct ttgcccgaag aattggtcca
     1621 gcgatatacc agcgacctgc agttgccgga atacgatgcc cgcgtcatcg tcagcgataa
     1681 agacagcgct gtttatttcg ggcggctcat cgcccatacg accaattata aggcggctgc
     1741 caactggatg ttaggcccgg tacgttccgg cctcaatgag ctgtcgctgg aatggaaaga
     1801 ccttcctgtg cctccggatg cgctggccga attgatccgg ctggtcgaag aagggaggct
     1861 gagcttttcc atcgcggctc agcgcatcta tcccgtcctc ggcctggaac cttcgcgcaa
     1921 acccctggat attgctatcg ccctcaacct gctgcaaagc tcggatacta ccgagatcgc
     1981 agtctggatc gaacaggctc tggcgggcat gcccgacaag gtcatggaat ataagaaagg
     2041 gaagaaaggt ctcatcggtc tcttcgtcgg agaagtaaaa aagctttcca aaggcaaagc
     2101 ggacccgagg ctcaccaatc aattattgct cgaaaaactg gaaaattaag atatgacaaa
     2161 gcacctctcc cgtatagtac cggtcgtcat gatggcctgt gttcttttca ccctctcttg
     2221 tcagccgaag aagaaaggga ccttcaccgt cgagggcacc tttaagaatg cggacaagct
     2281 gtccgccatg gcgggaccta tctcaaaagt ctatctcgag gaggtgtctt atggaaagga
     2341 tcaggcgccg gtgatcctcg attccgccag gcttccggtc accagtggca gcttcaccgt
     2401 gacgggcgca gtaaagacgc aggagatctt cgaagtggta ttcggttcca atgtcatcgc
     2461 tgttccgttg atcaatgacg cctccgagat acgcgtcaat gtcgatctgg gaaaacagga
     2521 cgatttctat actgtcagcg gttcggaagc cagcggtcag ctgaaagatc tgatcaccgc
     2581 ttttgggaaa aagaatttcg aggtagagaa gagcatggcc gatctcgaca gcctcaaaca
     2641 ggcgaatgca gccgacacac agctgcttgc cgcgaccgga gtgaagaaca atgccatcca
     2701 ggatctcaat atctatctca agcaggtggt caataccaac accaatgcga cgatatgcgc
     2761 gttggcacta agctgggcct cccgcagctt cccccggagc gacttcgaag cctccctgtc
     2821 cgatctggtg aggaaatatc ccgaaaatgg cgttttgacc gcgctaaggc aaagctacga
     2881 tctgcaaaag gaacagctgg tccagcagga agcgcatgac caggcgaata gctgggtcgg
     2941 caagcaagcc cccgatcttt cgctccccga tgcaaacgga aatacggtgt ctctttcctc
     3001 ctttaaagga aaatacctgc tcgtcgattt ctgggccagc tggtgcggac catgccgcgc
     3061 cgaaaatccc aatgtggtaa tcgcctacaa tcaattcaaa ggcaagaatt ttacgatcgt
     3121 cggggtttcg ctcgacaaag aaaaggatgc atggcaggag gcgatccgcg ccgacaagct
     3181 cgactggacc caggtcagcg atctcaaatt ctgggaaagc aaagccgtca agacctttaa
     3241 tttcgacggg atccctttca acgtccttat cgatccccag ggcaagatca ttgctcaggg
     3301 cctccgcggc gacgaccttg agaaggaatt gaagagcgtc ttgaaataat agaccgcgcc
     3361 gaaaataatc gtctgtatta gtcgttattg taatatggga ctgatcagac ttttattggc
     3421 gctggcagtc gttgccacgc attgcggacc tatattcggc tgtaatctgg ttggagggca
     3481 gatcgccgtt caatccttct ttattatctc gggtttttac atgtcggtca tattgaatga
     3541 aaaatatgtc ggtgtaaata actcgtaccg gcttttcata acgaataggt ttatgcgcct
     3601 ataccctatc tactgggcgg tatttgtggc caccatgctg gtctgtatag gcatttcata
     3661 cctcaacgcg gaccacaccg cgccggtttt cgtcaattac acgtcgttca aaaccagttg
     3721 gtttgcgtcg acatttttga tcctgatcaa cctcctgata ttcggacagg gccttgccat
     3781 gttcctgggt ctgaatccgg acggaaactt gtttctgacg gctaattttc ggaatactaa
     3841 cccgtatgtt tatacgtttt tgttcatccc ccaggcgtgg accctgggcc tcgagctgac
     3901 attttacctg atcgcgccgt ttatcctgcg cagggggtta aagactgtag ttccactcat
     3961 aatcggagct ttcttgctca ggatggtgct ctacgatcgt tttcatttgc agaacgatcc
     4021 atggacgtat cgctttttcc cgaccgaggt gatgttcttc ttactgggat acgtctccta
     4081 ccggatctac cttaagctaa agacaaaaca cattcccccg tcatactgca gagcgctgct
     4141 gatattcact gtcctcttta ccattttgta tacgcacctg ccgacgctga aattcggatt
     4201 cctcccgttt tccatgaggg attttgcata ctttaccacg atgttgatag ccatcccctt
     4261 gcttttcaat ttcctcaaga agaacaggct tgacagcaag atcggagaac tctcatatcc
     4321 catctatatt tcccatatgc tggttttgat gatctgcgat gtattcagcg aaaggctgtc
     4381 gtcaggtttc ttaaaagcaa gttgggtcgt ggcgttactg actatcgccg tctcctatgg
     4441 tttgaacatt gccattgcgg cgcccataga aaaatacagg caatccaggc tgacgaagac
     4501 ccgcctgcag ccggctatgc aaacgcctat acaattgagg ctgttctaac acatccccca
     4561 aagacaaaac ctcataaaca attgataaac aattgactat gaggctaatg tggtcccgtc
     4621 cagaatcgaa ctggaaccca gggcttcgga gacccttata ctatccattg tactacggga
     4681 ccaattcctt cgcggcaaac ggagtcgcgg cgaaaggagt gcgaaattag gcaatttctt
     4741 ctatttgtca ggattttaaa cgaacctctt gtaaaagctg gaaggcttat gctgctcctt
     4801 ctccggcggc gtcctattcc acaacatttc aaacgcccct tcctccggcc gcaccctcac
     4861 cacacaccca tagtgcagcg caaacgcctt aaagctgtcg atcagcggtg tacccgtgat
     4921 cccggcaagt atactacgga gaacgccacc atgggcaacg atgacctgcg ggccgccgcc
     4981 ggtcgcggcg atcgcggtaa aacaattcat cacgcgctca tgcaggtcaa gatagctctc
     5041 gccacccgga acgcggatat tgacgaaatc tgccatccag gggtcgatct cttctttggg
     5101 cagttgatcc cagggttgca tctcccagct gccgcaatgc agctccatca gctcccgccg
     5161 gagcgatacc ggtacaccgg gaaataaatg ctccgccaga agcgtacacc ttttcaacgg
     5221 actgctgtat acggtcatta tacccgctgg cagcgacttc aatatcatct ccgcctcctc
     5281 gcgaaagctt tccgtcacat ccaggtccgt ctgtccatag cagatgcctt tctctaccga
     5341 tggggtggta tgtcttatca gatagatctc cttcatagat agcgccaaat taccacaaat
     5401 cccaggtaaa tcgtgatttc ggacacttgt tgtatggcac cgagacaatc tccggtatac
     5461 ccgccgatcc aacggtaaaa aaaacgatac atcaaccacg ttgcgatcag cgcgggcgca
     5521 accgccagca aaaaaccggg tggcaacaag gcgaacggcg caagccccag cacgacagcc
     5581 gtcaccagtc cgccggtccc gatacgctga ctcgtcatcg atccggattt gctggtatcc
     5641 gtgtctcccg cataacgcga ggtctgcatc accagcaccg gcatgagtcg gctaagactg
     5701 tgtgcagcga tcagcgcaac gataaaaaaa ggaccgggcg tcaattcctt caatacgaga
     5761 aatttggtgc ccagtatcac tatcagcccg atggcgccga aggcgcctat gcggctgtcc
     5821 ttcatgatca gcaaaatctt ctcttttgac caaccgccgc caaatccgtc gcagacgtcg
     5881 gcgaaaccgt cttcatgaaa ggccccggtg acgagcacgc cggcgatcat cgaagccgca
     5941 ataccgattt cgacggatac ataacgggca aacaagaaat agaaaagcga gctgatcgcg
     6001 cccacgatcc agccgatgac gggaaaatat cgcggcgcct gccgcatgta ccctggcgaa
     6061 tggtcgaccg atgatggaac tctcagacgc gtcaggaaca tcacagctgt aaaaaatatc
     6121 cggacttctt ttttcatatc taagccttac tcacccccgc cgattcaaac gatgccatct
     6181 ccctcataaa atgtacagcc gactgcaaca gagggaatgc catcgcggcg cccgttcctt
     6241 ctcccagacg catacccaga tccagcaacg ggtcggcgcc gagatattcc agcatcctcg
     6301 cgtgtccgct ctctccgctg gcatgcgcga aaaaacaaca gtcctgtacg tcgctgcgga
     6361 gaagcctggc caataacagc gccgcggtac tgatgaaccc atccacgagg atcaccatcc
     6421 gaagctctgc cgctttcaga taggctccgg tcatcatcgc gatctcgaag ccgccgacat
     6481 gcgaaagaat ggtcatagcc ccgggatggg cggcaacggc cggcagatgc cgccgaaatg
     6541 catcccgaag gacctctttc ttcttcgtca attgttcatc attcgtgccg gtgccgcgtc
     6601 cgacacattc ttctaccggg ataccggtga catagctcat gatcagcgag gcggaggagg
     6661 tattgccgat acccatttca ccgaagccga cacaattgca tccggaggca tacaagtcgg
     6721 tcaccacttc tttccctgcc tcgacggccc gttcggcttc ttccggagtc atagccgcgg
     6781 tctcgagata attgcgggtg ccgtgaccga tcttcttaga gatcagcttc cccggaaaga
     6841 tcgctgcgtc gaaatcgaaa ttgacccccg catcgacaac gcaaagcccg atgtgatgga
     6901 gacgacataa gacattgatg gcagctcctc ccgtgatgaa gttcagcacc atctgtgccg
     6961 taaccgcctg cgggtaaggg ttgaccgttc ccgtcgcggc gatgccgtga tctcccgcaa
     7021 aaaccacgat atatggccgg ttcagctgcg gatgcagggt cccctggata agccccgttt
     7081 gaagagctat ttcttccaga cgtccgagcg cgccaagcgg cttggtcttg ttgtcgatga
     7141 catgttgcag cctttgccgc agtgtcgcct gtgtacctgt ttgcatggac ttatttattt
     7201 tgtttgacag tgagcggaat tccgctgatc ataaagatca cctcatcggc ccggcctgcg
     7261 atatactggt tcacccatcc ctgcagatcg gtgaatttcc ggcccgcttc tgtgtctgca
     7321 tgcattccca ttccgatctc attgctgatg ataatgatcc gggcctcctg ttgtaacaat
     7381 cggtcgacct cctgccggca gacctccaga caagcctcgg cgtcatagcg cagatcggaa
     7441 tagaaattat tgatccaaag ggtaacgcaa tcgatgaccg cggtctttcc gtctaatgat
     7501 aacccgccaa tgtatttttc ttcttccagc aaggtccacc tttcatcgcg atcctgccgg
     7561 tgccggtcga cccggttccg gaactcctcg tcccaaattc ttgcagtggc tacatagacc
     7621 gggtttccgc tgaattgcag ggcccgttcc tgggcgtacc ggctcttgcc gctgcgcgcc
     7681 ccgccggtta taaagatcat cataaatgta ctgcgatacc cgccatgaag ttaaatcgtt
     7741 tactgttaaa acccggtatg tcgaagtatt gcctatcggt taaattttcg agatcgagga
     7801 agaatttcca ggtctttgcc agccggtact ccgcatatcc gttaagcgtg tagtaggacg
     7861 cgaccggaat gggggcgctg ccatagacgg gttcgatcct tttgcccacc gtacggagcg
     7921 aaaggctgat atatagtccc tcatccggct gccagcccgc cttcaggttc aacagatttt
     7981 tcggctgccg gtataaatta tagaagctgg tgtccttgcc accggaaagg gtcgttccct
     8041 tcccggtaat ataggtataa ttcccggtca acgaccaggg acccgccttc cagcttactt
     8101 cccattccat accataatcg tcctggcggt cctggttgat atagaaccca ttgtaatagg
     8161 tagcatcgta ggtgaagatg atctcgtcct tcgtatgccg ctggaaaaat actccccgtg
     8221 cattgaaatg tttccggctg tattgtgccc ccatctccag gctctccgtt ttttcggggc
     8281 tcagctgtcc atacggatca ctatacggag aatacagctg gtacaacgtc ggtgcggaga
     8341 aacccgaaga caggtttgcg aacagcttca cgcgatcatc gatgacatac gacggatcga
     8401 tcgaatacgt gacgacgttt ccatatttgc tgaagccgtt gtatcgcccg ccgaggccaa
     8461 gattgaaacc gcccagctcc ttgatctgca gcgagacata tcccgccgat tgcctgaccc
     8521 tggcgctgtc gaacccgatc ttgctggtat ccgaataata ttgcgaaaga taaacgttgg
     8581 actgatcggt accttgatta cggtaatcaa tacccgccaa aaggtcgaca tgacgcgata
     8641 gcgcaagatt tgtgtacagc tcggcaaaat gcgaccggcc cgtatagctg ctttgactga
     8701 atgaaccgcc ctgtgcaatg gccatggcag agtcgtccag gtaattcctg
//
LOCUS       DASYSV010000159         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_31875, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000159 DASYSV010000000
VERSION     DASYSV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31875"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VGQ51_04920"
     CDS             complement(<1..1062)
                     /locus_tag="VGQ51_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV9035934.1"
                     /translation="MAEVILMPRLSDTMTEGVIAAWHKKVGDSVRKGELLAEVETDKA
                     TMDLESYKDGTLLYTGTDKGGKIQVNDLLAIIGAPGEDITSLVKNKGGAASPAPAAPA
                     AANNPAPQTAPAQASGIDLSKQEEVILMPRLSDTMTEGVIADWHKKVGEAVKKGDILA
                     DIETDKATMELESYKEGKLLYVGAQKGEKIAVNDLLAVIGDEKKVDLEKIVAASRNKG
                     VAGPQAAPATNAPAMGATAPAAGQTSSAAGNETQHSGMSVTADGRIKASPLARKMAAE
                     KGIDLSKVAGSGDNGRIIRKDVDSYTPGKAAAAVTPGSAPGKTVSPAVMGQESYDEVA
                     VSQMRKVIAKRLAESKFSAP"
     gene            complement(1108..>3014)
                     /locus_tag="VGQ51_04925"
     CDS             complement(1108..>3014)
                     /locus_tag="VGQ51_04925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9035935.1"
                     /translation="YTPKILDILSKYHVPAAFFLVGINAENNIPIVKREFREGHEIGN
                     HTFTHPNIAKVSTRRALLEMESTRLLIECITGRSTIMFRAPYNADFEPEKWEELIPVA
                     IARTKNYLDIGESIDPLDWEPGATADSIFYRCIARKNEMTKAGLSGNIILLHDAGGDS
                     RQATVDALPRIIEYFQRKGFHFTTVADILGKTRDEMMPPVPKGSGYYLLQINYYFAEL
                     GYWSSHILFSAFVVFMILSVSRVLFIAFIATKEHYREKRYGLAPFWGTDRSDAPLVSI
                     IVPAYNEEVNAVGSVESLLKCDYPNFEIIFVNDGSLDQTYQRVSETFAGNDKVKVYTK
                     VNGGKASALNYGISRSKAEFVICIDADTKLKSDAVSLLMEHFGKERQGGRKEGPVGAV
                     AGNVKVGNQVNLLTRWQAIEYISSQNFDRKAFSYLNAITVVPGAIGAFRKKAIEDAGG
                     FTTDTLAEDCDLTVRILRCGYVIDNDNRAIAMTEAPETLKQFFKQRFRWSYGVMQTFW
                     KNRDALLNWKYRWLGWAALPNILIFQYIIPFVIPLADFFMIVGLLTGNASKIGGYYLV
                     FMLVDLAVGLLAFIFEREKLIRLIWLIPQRLIWRWLTWLVLFRAVRRALKGELQHWGV
                     LKRTGNVKVV"
BASE COUNT          615 a          924 c          724 g          751 t
ORIGIN      
        1 cggggcggag aatttactct cggcaagacg ttttgcgatc accttcctca tctgcgagac
       61 agccacctcg tcataactct cctgtcccat tacggcagga gatacagtct tacccggcgc
      121 gctccccggc gtaacggcag ctgcggcctt acccggcgta tagctatcga cgtccttcct
      181 aatgatacga ccattgtcac cgctgcctgc aaccttcgag aggtcgatcc ctttctcggc
      241 agccatcttc cttgccagcg gcgaagcctt gatgcgtccg tcggcagtca ccgacatacc
      301 actatgctgg gtttcgttac cggcagccga tgatgtctgt cccgcagcgg gtgccgtagc
      361 gcccatagcc ggcgcatttg tcgccggcgc agcctgtggt cctgcaacgc ctttattcct
      421 ggaggccgcc actatcttct ccagatcgac ctttttttca tccccgatca ccgccagcag
      481 atcgtttacg gcgatctttt cgcccttctg cgcgccgaca tataacagct ttccttcttt
      541 atagctctcc agttccatcg tcgccttatc cgtttcgata tcggcgagga tatcaccctt
      601 ctttaccgcc tcacctactt tcttatgcca atcggcgatc acaccttctg tcatcgtatc
      661 gctgagccgc ggcatcagga tcacttcttc ctgtttggaa agatcgatac ccgatgcttg
      721 cgccggtgcg gtctgcgggg cagggttatt ggcggcagcc ggtgctgcgg gagcaggcga
      781 ggccgccccc cctttattct tgaccagcga ggtgatatct tctcccggag cgccgattat
      841 cgccagcaga tcgttcacct ggatcttgcc tcccttgtcc gtgccggtat ataataaggt
      901 cccgtcttta taactttcca ggtccatcgt cgccttgtcc gtctccactt ccgccaatag
      961 ctcgcctttc ctgacggaat cgcctacctt cttatgccac gcagcgatca ccccttcggt
     1021 catcgtatcg ctcaaacgag gcatcaaaat aacttcagcc ataacaattt tccggttttt
     1081 tggagccgtg aaattaagac aaaattgtca aaccactttt acattcccgg tcctcttcaa
     1141 cactccccaa tgctgcagtt cacccttcag cgccctccgg acggccctga ataacaccag
     1201 ccaggtcagc cagcgccaga tcagccgctg agggatcagc cagatcagcc ggatcagttt
     1261 ttccctctcg aagataaagg ccagcagtcc cacggccaga tccaccagca tgaaaacgag
     1321 gtagtatccg ccgatcttcg aagcattgcc cgtcaacaag cctacgatca taaagaaatc
     1381 ggccagcggg atcacaaaag gtataatgta ttggaagatg aggatattgg gtaacgccgc
     1441 ccatccgagc caacgatatt tccagtttag cagcgcgtcc ctgttcttcc agaaggtttg
     1501 catgacgcca tagctccacc ggaaacgttg cttgaagaat tgtttcaggg tctcgggcgc
     1561 ttccgtcatg gcgatagcgc ggttgtcatt gtcgatgaca tatccgcaac gcaggatccg
     1621 aaccgtgaga tcgcaatcct cggccagcgt atcggtcgtg aaccctccgg catcctcgat
     1681 cgctttctta cggaaagccc ctatagcgcc cggaacgacc gtaatggcat tgagatagga
     1741 aaacgccttc cggtcgaaat tctggctgct gatatattcg atggcctgcc agcgtgtgag
     1801 caggtttacc tggttgccga ccttgacatt gcccgcaacg gcgccgacag gaccttcctt
     1861 tctccctccc tgacgctcct ttccaaaatg ctccatcagc aggctcacgg catcggactt
     1921 tagcttcgta tccgcgtcga tacagatgac gaattcggct ttgctccggc tgatgccata
     1981 attgagcgcg gacgccttcc ccccgttgac cttggtatag acctttactt tgtcatttcc
     2041 ggcaaaagtc tcactgaccc gttgataggt ttggtccagg cttccgtcat tgacgaagat
     2101 gatctcgaaa ttcggataat cgcatttgag caggctttcc accgaaccca ctgcgttcac
     2161 ttcctcattg taagccggga cgatgatgct tacgaggggt gcgtcggatc tgtcggtccc
     2221 ccaaaatggc gccaggccat accgcttttc ccggtaatgt tccttggtgg cgataaaggc
     2281 gatgaacagc acgcggctca ccgacaggat catgaatacc acgaaggcgg agaaaaggat
     2341 atggctgctc caatacccca gctccgcgaa ataataattt atctgcaaca ggtagtaacc
     2401 gctgcctttc ggtaccggcg gcatcatctc atcccgggtc tttccgagga tatccgccac
     2461 cgttgtgaaa tggaaaccct ttcgctggaa atattcgatg atccgcggca gggcatctac
     2521 tgtcgcctgc cggctgtccc cgcctgcgtc atgcagcaga atgatattcc cgctcaggcc
     2581 tgccttggtc atttcgttct tacgcgcgat acatctgtaa aagatgctgt cggctgtcgc
     2641 ccctggctcc cagtccagcg ggtcgatcga ttccccgatg tccaggtagt tcttggtccg
     2701 cgcgatcgcc accgggatca gctcttccca tttttccggc tcgaagtcgg cgttatacgg
     2761 cgcacggaac atgatcgtcg atctcccagt gatacactcg atcagcagac gcgtcgattc
     2821 catttccagc agggcccttc gggtactcac cttcgcaatg ttgggatgtg taaaggtgtg
     2881 attgccgatc tcatgccctt ccctgaactc ccgcttgacg atcggtatat tgttctccgc
     2941 gttgatccct accaggaaaa aagctgccgg aacatggtat ttgctcagta tatccaatat
     3001 tttgggtgta tact
//
LOCUS       DASYSV010000160         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1563890, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000160 DASYSV010000000
VERSION     DASYSV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563890"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VGQ51_04930"
     CDS             <1..1123
                     /locus_tag="VGQ51_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV9035936.1"
                     /translation="VTDVVLEKDVMLHFPYHSFNPLIDMLREAAIDPNVTTIKITGYR
                     LASNSKIINALVNAVRNGKHVTVMLELRARFDEEANLEWKDRLEDEGVRVLIGIPNVK
                     VHAKICLIKKRINNFTIHYGFVSTGNLNEKTARVYGDHCLLTSDRHIMADVNRIFNYL
                     ENWKEGTAPLKACKILVPCPTSLRRELARMIDRETKAAREKKPASMILKMNSLSDESL
                     IDKLYEAARAGVKIQLIIRGIFCMLSENNKFLIPVKAISIIDEYLEHARVFIFHNGGN
                     EKTYISSADWMVRNLDHRVEVTCPVLDEGICQELKEILNIQLSDNVKARWLDNELLNR
                     YKRDHPEDRVRSQIEIYNYLYQKAIPVGERTGQEHAVTG"
     gene            1150..2052
                     /locus_tag="VGQ51_04935"
     CDS             1150..2052
                     /locus_tag="VGQ51_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEV9035937.1"
                     /translation="MKMRLAAIDIGSNAARLLISDVSDNFGKPAFNKINLVRVPLRLG
                     FEVFDNKEISSEKIDMILHTIKAYKHLIEAYEVDHIKAAATSAMRDAVNAESIIHRVK
                     EETGIGIEIISGGYEASLIYENHIAENMDTEHAYLYIDVGGGSTELTFFAGNDLLFSS
                     SFNIGTIRLLKNQVSVAQWDNMKEYIRRETRNFADRVVAIGSGGNINKVFSLSKRKEG
                     KSLNIELLKDYYKEFSSFTLEDRMRLYKLREDRADVIVPALLIYVNTMRWAGAQEIYV
                     PKIGLADGLIQHLYADLRKKGVEV"
     gene            2086..2901
                     /gene="panB"
                     /locus_tag="VGQ51_04940"
     CDS             2086..2901
                     /gene="panB"
                     /locus_tag="VGQ51_04940"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEV9035938.1"
                     /translation="MSVHTEIKKVTTHSLLRMKASGEKISMITAYDYSFARIFDAAGI
                     DVILVGDSASNVMAGHETTLPITLDQMIYHASSVVRGVNRAFVVVDLPFGSYQSNSKE
                     ALASAIRIMKETGAHGIKCEGGEEIMESVKRILAAGIPVIGHLGLTPQSIYKFGTYSV
                     RAKEQTEAEKLRRDAGLLEEAGCSGIVLEKIPAQLARIVAQSLQIPIIGIGAGKYCDG
                     QVLVMHDLLGINTEFQPRFLRRYLRLHEEITDAVKQYIHDVKKNDFPNEQEQY"
BASE COUNT          818 a          863 c          837 g          657 t
ORIGIN      
        1 ggtcaccgat gtcgtgctgg aaaaggatgt catgctgcat ttcccctatc attccttcaa
       61 cccgctgatc gatatgctgc gggaggccgc gatcgacccg aatgtcacca ccatcaaaat
      121 aacgggttat cgcctggctt cgaacagcaa gatcatcaac gccctcgtca atgccgtccg
      181 caacggcaaa catgtgacgg tgatgctgga gctgcgcgcc cgttttgacg aggaagccaa
      241 tctcgaatgg aaggacaggc tcgaggatga aggggtccgg gtgctcatcg gtattccgaa
      301 cgtgaaggtg catgcgaaga tctgtctcat caagaaaagg atcaacaatt ttaccatcca
      361 ctacggtttt gtcagcacgg gcaatctcaa tgaaaaaacg gcccgcgttt atggcgatca
      421 ttgtctgctc acttccgacc ggcatattat ggctgacgtc aaccggatct tcaattacct
      481 ggaaaactgg aaggagggca ctgctccgct gaaggcctgc aagatcctgg tcccctgccc
      541 caccagcctg cggcgggaat tggccaggat gatcgaccgc gagaccaaag cggcccggga
      601 gaagaagcct gcatccatga tcctcaagat gaactctctt tccgacgaaa gccttatcga
      661 caagctctat gaggcggcga gagcaggtgt gaagatccaa ttgatcatcc gcggcatctt
      721 ttgcatgttg tcggagaaca acaaattcct gatcccggtg aaagccatca gcatcatcga
      781 cgagtatctc gaacatgcgc gggtatttat ctttcacaac ggcggaaacg aaaaaactta
      841 catatcttcg gccgactgga tggtgcgaaa cctggatcat cgcgtggagg tcacctgccc
      901 ggtcctggat gaaggcattt gccaggagct gaaggagata ctgaacatcc agctttcgga
      961 taacgtgaaa gcgcgctggc tggataacga gctgttgaac agatacaaac gggatcaccc
     1021 ggaagacagg gtcaggtccc agatcgagat ctataattat ttgtaccaaa aagccatacc
     1081 cgtcggggaa agaaccgggc aggaacatgc ggtcaccggg tagaggaagg aggctgagaa
     1141 acctacaaga tgaaaatgcg acttgcagcg atcgacatcg ggagtaatgc ggcacggctg
     1201 ctgatctcgg atgtgtccga taattttggt aagccggcgt tcaacaagat caatctggtg
     1261 cgcgtccccc tccgcctggg gttcgaggtc ttcgacaata aagagatctc ttcggaaaag
     1321 atcgacatga tcctccatac gatcaaggcg tacaagcatc tcatcgaagc atatgaagta
     1381 gaccatatca aggccgccgc cacgtcggcc atgcgcgatg ccgtgaatgc ggaatcgatc
     1441 atccaccgcg ttaaggaaga aacgggcatc gggatcgaga tcatcagcgg cggctatgag
     1501 gcatccctca tctatgaaaa ccacattgcg gagaacatgg ataccgaaca tgcctatctc
     1561 tatatagacg tgggaggcgg tagcacggag ctgacctttt tcgccggtaa cgatctgctg
     1621 ttctcatcca gcttcaatat cggcaccatc cgtctgctga agaaccaggt gtccgtggcg
     1681 caatgggaca acatgaagga atatatccgg cgggagaccc gcaatttcgc cgacagggtc
     1741 gtggccatcg ggtccggcgg caacatcaac aaggtcttct cgctctccaa acgcaaggaa
     1801 ggcaagtcgc tcaacatcga attgctgaag gattactata aggagttctc cagcttcacc
     1861 ctcgaagaca ggatgcgtct gtataaatta agggaggacc gcgccgacgt gatcgttccc
     1921 gccttactca tttatgtcaa taccatgcgg tgggccggcg cgcaggagat ctatgttccc
     1981 aagataggac tcgcggacgg gctcattcag catctctatg ccgacctccg gaaaaaaggc
     2041 gtcgaagttt aaaaattacg cataccttgc ggcaaaccaa gggccatgtc agttcatacc
     2101 gagatcaaaa aggtaaccac ccattcgctg ctgcgtatga aggccagcgg ggaaaagata
     2161 tcgatgatca ctgcctatga ctattctttt gcgcggatat tcgacgcggc gggcatcgat
     2221 gtcatcctcg tcggcgactc cgcctccaac gtcatggccg gtcatgaaac cacgcttccg
     2281 atcaccctcg atcagatgat ctatcacgct tcctccgttg tcaggggggt caaccgggcg
     2341 ttcgtcgtcg tcgacctgcc attcggttct taccagtcca attccaagga agcgctcgct
     2401 tcggctatcc ggatcatgaa ggagaccggg gcccatggga tcaaatgcga gggcggcgaa
     2461 gagatcatgg aatccgtcaa aaggatactg gcggccggga tacccgtcat cggccatctc
     2521 ggacttaccc cgcaatccat ttataagttc ggcacctata gtgtaagggc caaagagcaa
     2581 accgaagccg agaagcttag aagggacgcc gggctcctcg aagaggccgg atgttccggc
     2641 atcgtgctgg aaaagatacc cgcccaactt gcccgcatcg tcgcacaaag cctgcagatc
     2701 cccatcatcg gtatcggagc cggtaaatac tgcgacgggc aggtactcgt catgcacgac
     2761 ctgctgggta taaacacgga gttccaaccc cgcttcctcc gtcgctatct gcgtctgcac
     2821 gaggagatca ccgatgccgt caagcagtat atccatgacg tcaagaagaa cgatttcccg
     2881 aatgaacagg agcaatatta gccgctgccg gaacggtatg gcatcccgcg gacccgcagc
     2941 cctggcgacg atgcccactc accgatacca attccccatt catctaataa atgcgggata
     3001 aaggccaaac ccaattagat ttgctaaggt taatagcaat caagcgtgaa gaaacttacc
     3061 gtacagatag tcatccatat agcagcctgg ttatgcttcc tgctgctccc gatcctgttc
     3121 tatccccggc cgcgggaggt agactataac tttttcgtag agcatttcct gacgc
//
LOCUS       DASYSV010000161         8036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2461573, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000161 DASYSV010000000
VERSION     DASYSV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461573"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VGQ51_04945"
     CDS             complement(<1..843)
                     /locus_tag="VGQ51_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HEV9035939.1"
                     /translation="MIALLGAFLRLVRWPNLVFIFLTQLLFYYFIQLPVHDQPFGEFP
                     LVLRPRLFYLLAASSVLIAAAGYIINDYFDLNIDRVNKPNRLVVDRIIRRRSTILWHW
                     LLSGLGLLLSFYVSWRIRNPLVAVGNLGCVILLYFYSTTFKRKMLIGNIIISLLTAWV
                     ILVLYIAELYWPGFTDNDYRRFLSTLFKFAIVYSSFAFLLSMIREVIKDIEDMDGDMK
                     YGCRTMPIVWGVNVAKVFAGTLIVVLLGALVILQFYVLQKAWFPLTMYGVLLVDLPLL
                     FILRR"
     gene            complement(855..1379)
                     /locus_tag="VGQ51_04950"
     CDS             complement(855..1379)
                     /locus_tag="VGQ51_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HEV9035940.1"
                     /translation="MMSVLEKFKSIRTFVFDVDGVMTDGSVLVFETGEQVRRMSTRDG
                     YSLQLAIKKGYRVVVISGAASEGVRQRLQYLGIRDIFLSVHDKVDVLRKYTLDHRLEE
                     KDMLYMGDDIPDYAPMQYVGLGCAPSDAAAEIKHIAAYISSYGGGQGCVRDVIEKVLK
                     LNGNWELDQSLGSK"
     gene            complement(1429..2220)
                     /locus_tag="VGQ51_04955"
     CDS             complement(1429..2220)
                     /locus_tag="VGQ51_04955"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEV9035941.1"
                     /translation="MKVVIIGSGNVATALGRTILTAGHVIVQVAGRNRETVVRLAEEW
                     GAHPTTGWGTVDKTADIYLVALSDSALPGLGKALSLPSKLVLHTSGALPADTLRDVTD
                     RAGVLYPLQSFGRGIAPPEEFPLLIDAVRPEDIPLIRGFAGTLATQVVRADDAVRLKL
                     HVAAVLVNNFTNHLYTLAADFCRQENIDFHLLLPLIRETARRVGSTDPRDVQTGPAIR
                     GDGITLEKHRQSLSNYHNIKQLYDLFTILIEEYYRSLDGRGRPLE"
     gene            complement(2230..2886)
                     /locus_tag="VGQ51_04960"
     CDS             complement(2230..2886)
                     /locus_tag="VGQ51_04960"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEV9035942.1"
                     /translation="MRTFEDSYRHKGLRRQLVHVIREKGITDERVLDAILHIPRHFFM
                     DSAFDKIAYEDRAFPIGENQTISQPYTVAYQTQLLEVKPFERVLEIGTGSAYQACVLA
                     EMKAQVYTIERQKKLFDANKQFQYLKRYPALKFFYGDGYEGLPTFAPFDKVIVTAAAP
                     FIPPRLIEQLKPGGRMVIPVGEGQVQRMLRLTRQEDGSATQEVFDNFSFVPMVEGKNS
                     "
     gene            2935..4122
                     /locus_tag="VGQ51_04965"
     CDS             2935..4122
                     /locus_tag="VGQ51_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HEV9035943.1"
                     /translation="MKFKTGSQLAFMMFVEYFIWGAWYVTMGTYMLSHLHTSGTLVGK
                     AYAALAAATIISPFFVGRIADRYFPAQRVMGILHLLGAVVLFCATRVTDGDIFKWMIL
                     LYSLLYMPTIALSNSVAFHQMSDPGRQFPWIRVFGTAGWIVAGILIGVWKIEMTATTF
                     NIAAIASACLGIVSFGLPDTPPKGAESKTRVKEALVLFKSIPYVIFFIAAILVCIPLS
                     FYYGFANPFLNELGMTDAAAKMTLGQFSEGLFILAIPFLFNRIGVKNMLLMGMMAWLL
                     RYVCFAYGSLGAHAWMLYAGIILHGVCYDFFFVTGYMYTEKKAGEKIKNSAQQLFTMA
                     TYGAGMLIGTWVSGLVVDRNIVSATSHDWKSIWLVPAYIAAGVLVYFILFFREKKQLT
                     ANV"
     gene            4134..5327
                     /locus_tag="VGQ51_04970"
     CDS             4134..5327
                     /locus_tag="VGQ51_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9035944.1"
                     /translation="MANIAMLGSGFIGRFYADSIVGQRSRDSIISIYSRRKESAEKFA
                     KDYHCKYVSTDMEEAIAHRDVDLVCISLPNNLHEIAVLLCCKHKKAVMTTKPLGRNAT
                     EARRMLEAVEDAGIFNAYLEDLVYTPKFLKAHASVQNGALGRILWAKSRETHPGPHSE
                     WFWDIEQAGGGCILDLGCHCVEIARSFIGKDIRPVEVMCWADTQVKPIDAEDHAIGLV
                     KYENGAIGQFEVSWTFRGGMDLRDEVMGTEGTVWINNFLRTGLEMFTTGKGADYVAEK
                     AESNTGWLFPVGDEVNDLGYNHMFTDVFNALEKGGTPKETFYDGYIVNAVLDAAYRSA
                     KTKLWEPVRLDIWRGRIGVTRDSHLTEYDAEHYLIKEETTHYGARKLILKSKGDGKIS
                     EKILQ"
     gene            5430..6401
                     /locus_tag="VGQ51_04975"
     CDS             5430..6401
                     /locus_tag="VGQ51_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase small
                     subunit"
                     /protein_id="HEV9035945.1"
                     /translation="MQNTNELLLKENKDRFVILPINFPKVWEMYKKHEASFWTAEEID
                     LGADMQDWQRLNDGERHFISHVLAFFAASDGIVNENLAVNFMSEVQLPEARCFYGFQI
                     MMENIHSETYALLIDTYIKDPQEKYRLFHAIDTVPAVKKKAEWALRWIQNGSFAERLV
                     AFAAVEGIFFSGSFCSIFWMKKRGLMPGLTFSNELISRDEGLHCEFACLLYSMLENKL
                     SHDQVATIIGDAVRIEKEFITEALPVDLIGMNARLMQQYIEFVADRWLGELGYPRLFN
                     VSNPFDFMEMISLQGKTNFFEKRVGDYQKSGVMSGKEAQTFSLEEDF"
     gene            6424..>8036
                     /locus_tag="VGQ51_04980"
     CDS             6424..>8036
                     /locus_tag="VGQ51_04980"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEV9035946.1"
                     /translation="MFVIKRNGKRESVKFDKITARIEKLCYGLDRRFVNSIDVAKKVI
                     EGLYDGVTTTELDNLAAETAASLTVKHPDYALLASRIAVSNLHKNTIKSFSSTMRLLY
                     ECQDSKIGKATPLLSEEVWQIISDHAELLDSTIIYDRDYGFDYFGFKTLERSYLLKVE
                     GKVVERPQHLYMRVAIGIHHHDIEGVIRTYHLMSERWFTHATPTLFNAGTPKPQMSSC
                     FLLTMKDDSIDGIYDTLKQTAKISQSAGGIGLSIHNIRATGTYIGGTNGTSNGIIPML
                     RVFNDTARYVDQGGGKRKGAFAIYLEPWHADVFEFLDLRKNHGKDEMRARDLFYALWI
                     PDLFMQRVETGGNWSLFCPHEAPGLHESWGKEFEDLYTRYESEGRARRTVRAQDLWFA
                     ILDAQIETGTPYLLYKDAANRKSNQQNLGTIRSSNLCTEIIEYTSPDEVAVCNLASLA
                     LPRFVTGGVFDHQKLYEVTYEVTKNLNKIIDHNFYPIEEARNSNLRHRPIGLGVQGLA
                     DAFILMRLPFESERARQLNKEIFETIYFAA"
BASE COUNT         1915 a         2210 c         2119 g         1792 t
ORIGIN      
        1 cctcctcaat ataaaaagca acggaagatc gaccagcagc acaccataca tcgtcagcgg
       61 aaaccacgcc ttctggagga cataaaattg caggatcacc aaggcgccca acaagaccac
      121 gatcaacgta cccgcgaaga ccttggcgac atttacgccc catactatgg gcatggtccg
      181 gcatccgtat ttcatatccc catccatatc ctcgatgtct ttgatgactt cccggatcat
      241 cgaaagcagg aaggcaaaac tgctgtacac tatcgcgaac ttgaacaagg tcgacaggaa
      301 ccgccgatag tcgttgtctg taaatcccgg ccaatagagc tccgcgatat acaacaccag
      361 gatcacccag gccgtgagca gcgagataat gatgtttccg ataagcattt ttcgtttgaa
      421 tgtcgtactg tagaagtaca gcaggatcac gcagccaaga ttgccgacgg cgacgagcgg
      481 attgcggatc cgccaactta cataaaagct cagcagcaac ccgagtccgc tgagaagcca
      541 gtgccacagg atcgtcgacc tccgcctgat gatacgatcc accaccagcc ggttgggttt
      601 gttgacccta tctatattca gatcgaaata atcgttgata atgtaccctg ccgctgcgat
      661 cagcaccgag gaggctgcca gcagatagaa caatcgtggt ctgagcacca gcgggaactc
      721 cccgaatggc tgatcgtgta ccggcagctg aatgaaataa tagaacagca gctgggtgag
      781 aaagatgaac actaaattgg gccatctgac caggcgtaga aaagcaccga gcaatgcgat
      841 catacttaat caaattattt cgagccgaga gattgatcca gttcccaatt accattcagt
      901 ttcagcactt tttcgatgac atcccgcacg caaccctgtc cccctccata cgaagatata
      961 taggcggcta tatgcttgat ctccgcggcg gcatccgaag gagcgcaacc gagtcctacg
     1021 tattgcatgg gggcatagtc cggaatgtcg tcccccatat acagcatgtc tttttcttca
     1081 agccggtgat cgagcgtata cttccgcagg acatccacct tatcatgaac gctcagaaag
     1141 atgtcccgga tgccaagata ctggagacgc tgccggaccc cttccgaggc ggcacccgag
     1201 atcaccacca ccctgtatcc ttttttaatg gccagctgta gcgaatatcc gtcgcgggtg
     1261 ctcatccggc gaacctgctc accggtctcg aataccagga cgctgccgtc ggtcatgacg
     1321 ccgtccacgt cgaatacaaa agtgcggatc gatttgaatt tttccaatac actcatcatg
     1381 ggataattat atacgatata cgttcgaaag cgagcaaaaa taccttaatt actccaaagg
     1441 ccgcccgcgc ccatcaaggg accggtaata ttcctcgatt aaaatcgtaa atagatcgta
     1501 tagttgttta atattatggt aattagatag cgattgccgg tgtttttcca gggtaatgcc
     1561 atcgcctcgt atggccgggc ccgtctggac atcccgcgga tcggtggacc ctacccgcct
     1621 ggcggtttcc cggatcaatg gcagcaggag atggaaatct atgttttcct gccggcagaa
     1681 atcggctgca agagtataca gatgattggt aaaattgtta accagaacgg ccgctacatg
     1741 caatttcaac cggacggcgt catctgcccg aacaacctgt gtcgccagcg tgccggcaaa
     1801 gccccggatg agcggaatat cttccggccg gactgcatcg atcagcaaag ggaactcttc
     1861 cggcggcgcg atccccctcc caaacgattg caagggatag agtacccccg cacgatccgt
     1921 cacgtcgcga agcgtgtctg cgggaagcgc cccggaggta tgaaggacaa gttttgaagg
     1981 caggctgagc gctttgccga gaccggggag cgcactatcg ctcagcgcga ccagatagat
     2041 atccgccgtc ttgtcgaccg tcccccaccc ggtcgttgga tgggctcccc attcttccgc
     2101 gagccggacc actgtctccc tgttgcggcc ggccacctgg acgatgacat gtcccgccgt
     2161 caggatggtc ctccccaggg cggtggcgac attccccgat ccgatgatga ctaccttcat
     2221 agatctcttt tagctgtttt taccttcgac catcggcacg aacgaaaaat tgtcgaagac
     2281 ctcctgcgta gcgctgccgt cctcctgtct ggtcagtctc agcatgcgct gcacctgtcc
     2341 ttctcctaca gggatcacca tccgaccgcc cggtttcagc tgttcgatca gccggggagg
     2401 gataaaaggc gccgccgccg tgacgatcac cttatcgaag ggcgcgaagg tcggaaggcc
     2461 ttcataaccg tctccataga agaacttgag ggcgggatat ctcttcaggt actggaattg
     2521 tttgttggcg tcgaacaatt tcttctgccg ctcgatagta tacacctgcg ccttcatctc
     2581 tgcgagtacg caggcctggt aggcgctgcc ggtgccgatc tcgaggactc tctcgaacgg
     2641 tttgacttcg agcagctggg tttgataggc aacggtataa ggttgcgaga tggtttgatt
     2701 ttctccgatc gggaaggcgc ggtcctcata agcgatcttg tcgaaagccg aatccataaa
     2761 aaaatgtctc gggatatgca ggatggcatc caggacgcgt tcgtccgtaa tgcccttctc
     2821 gcggataacg tgtaccagct gtctgcgcaa ccctttatgc cggtaactat cttcgaatgt
     2881 tctcatcgga tgcaatttaa tacctatatt cgatccacct aattcaccag taaaatgaaa
     2941 tttaagaccg gtagccagct ggccttcatg atgttcgtcg aatattttat ctggggcgcc
     3001 tggtatgtga cgatgggtac ctatatgctc tctcatttgc acacctccgg cacgctcgtg
     3061 ggcaaggctt atgcggctct cgctgccgcc acgatcattt ctcccttttt cgtagggcgc
     3121 atcgcggacc ggtattttcc ggcgcagcgg gtcatgggca ttctccacct gctcggcgcc
     3181 gtcgtgcttt tctgcgctac cagggtcacc gacggcgata ttttcaaatg gatgatcctg
     3241 ttgtattcgt tgctttacat gcctacgatc gcgctgtcga acagcgtagc ctttcatcaa
     3301 atgagcgatc ccggaagaca attcccctgg atccgggtgt tcgggacagc cggatggatc
     3361 gtcgcaggga tcctgatcgg cgtatggaag atagaaatga cggccaccac cttcaatatt
     3421 gccgcaatag cctcggcatg cctgggcatc gtcagctttg gtctgccgga tacaccgcca
     3481 aaaggcgccg aatcgaagac gcgtgtcaag gaagctctgg tactcttcaa aagcatacct
     3541 tatgtgatct tcttcatcgc cgccatcctg gtctgtatcc cgttgtcctt ctattatggc
     3601 ttcgccaacc cttttctgaa tgaattggga atgaccgacg ccgcggccaa gatgaccctc
     3661 ggacaattct cagaagggct gttcatcctg gccattccct tccttttcaa ccgcatcggc
     3721 gtaaagaata tgctgttgat gggtatgatg gcctggttgc tgaggtatgt ctgttttgcc
     3781 tatggtagcc tgggcgctca tgcctggatg ctgtatgcag ggatcatcct gcatggcgta
     3841 tgctacgact ttttcttcgt cacgggatat atgtatacgg agaagaaggc aggggaaaag
     3901 atcaagaatt ccgcgcagca gctgtttacg atggctacct acggtgcggg catgctcatc
     3961 ggcacctggg tatcggggct ggtcgtcgac cggaatatcg taagtgcgac cagtcatgac
     4021 tggaaatcca tctggctcgt acccgcctat atcgccgcgg gtgtcctcgt ctatttcatc
     4081 ctgttcttca gagaaaagaa acaactcact gctaacgtct aaaacgaacg tctatggcta
     4141 acattgctat gcttggctcc ggcttcatcg gccggtttta tgccgactct atcgtgggac
     4201 aacggagccg cgactccatt atcagtattt actcgcgccg caaggaaagc gccgaaaaat
     4261 ttgcgaaaga ttatcattgc aagtacgtgt ccaccgatat ggaagaggcc atcgcacatc
     4321 gcgacgtcga cctggtctgt atcagtctgc ccaacaatct tcatgagata gccgtcctgc
     4381 tttgctgcaa gcataagaag gccgtgatga ccaccaagcc gctcggccgc aatgccacgg
     4441 aagcgcgacg catgctcgag gcggtcgaag acgccggcat ctttaatgcc tatctcgaag
     4501 acctcgtcta taccccgaaa ttcctgaagg cgcacgccag cgttcagaat ggcgcccttg
     4561 gccggatctt atgggcgaag tcacgcgaga cgcatcctgg cccgcatagc gaatggttct
     4621 gggatatcga gcaggcgggg ggtggatgta tcctcgatct gggttgtcat tgcgtcgaga
     4681 tcgcgcgcag cttcatcggc aaggacatcc gccctgtcga agtcatgtgc tgggccgata
     4741 cgcaggtgaa acccatcgat gcggaagacc acgccatcgg gctcgtaaaa tatgagaacg
     4801 gggccatcgg tcagttcgag gtcagctgga ccttccgcgg cggcatggac ctccgcgatg
     4861 aggtcatggg taccgaaggg accgtctgga tcaacaactt ccttcgtacg ggtctggaga
     4921 tgttcaccac cggcaaaggc gcggattatg tggcggaaaa agcggaaagc aacaccggct
     4981 ggctcttccc ggtgggcgat gaggtcaacg atctcgggta taaccatatg ttcaccgatg
     5041 tgttcaatgc attggaaaaa ggcggcacgc cgaaggagac cttttatgac ggctatatcg
     5101 tcaatgccgt cctcgacgct gcttaccggt ctgccaaaac gaaactatgg gagccggtac
     5161 gtctcgatat ctggcgcgga cgcatcggcg tcaccaggga cagccatctc accgaatacg
     5221 atgccgagca ctacctcatc aaggaagaga cgacacacta tggtgcgcgc aaactgatcc
     5281 tgaaaagcaa gggggacggg aagatctcgg aaaagatcct gcaataaccg cgctccgcgc
     5341 ggaccttcag atcgacggaa aaagttaagc acatgcagtg gataaacatg ccccggctat
     5401 gtccggggca ttgttatttt tgacgtccca tgcaaaacac gaatgagctc ttactgaaag
     5461 aaaacaagga ccggttcgtc atcctcccga tcaatttccc gaaggtgtgg gaaatgtata
     5521 agaaacacga ggccagtttc tggacggccg aggagatcga tctcggcgcg gacatgcagg
     5581 attggcagcg cctgaatgac ggtgagcggc atttcatttc gcatgtgctg gccttttttg
     5641 cggccagcga cggcatcgtc aatgagaacc ttgccgtcaa tttcatgagt gaggtccagc
     5701 tgccggaagc ccgttgtttc tatgggttcc agatcatgat ggagaacatc cattccgaga
     5761 cctatgccct gctgatcgac acctatatca aggacccgca ggaaaaatac cggcttttcc
     5821 atgccatcga tacggtgcct gccgtgaaga agaaggcaga atgggcattg cgctggatac
     5881 agaacggcag ctttgccgag cggctggtcg cctttgccgc cgtggaaggc atctttttca
     5941 gcggcagctt ctgctctatt ttctggatga agaaaagagg attgatgccg ggtctcacct
     6001 tctccaacga gctcatcagc cgcgacgaag gcctgcattg cgagtttgcc tgtttgctat
     6061 actccatgct ggagaacaag ctttcgcacg accaggtagc cacgatcatc ggcgatgcgg
     6121 tgaggataga aaaggaattc atcacggaag cgttgcctgt cgatctcatc gggatgaacg
     6181 cccggctgat gcagcaatat atcgaattcg tggccgatcg ctggctggga gagctgggtt
     6241 atccacgact gttcaatgtc tccaatccgt tcgactttat ggaaatgatc tcgttacagg
     6301 ggaagacgaa tttcttcgaa aaacgcgtcg gcgattacca gaaatcgggg gtcatgtcgg
     6361 ggaaggaagc gcagacgttc agtttggagg aagatttcta agaactcatt aaattctaca
     6421 tatatgtttg tcatcaaaag aaatggcaag cgggaatccg tgaaattcga caagatcacg
     6481 gcccgcattg aaaaactatg ctatggtctg gaccgccggt tcgtcaactc gatcgacgtc
     6541 gccaaaaagg tgatcgaagg actctatgac ggcgtcacta cgaccgagct cgataacctg
     6601 gccgccgaaa ccgctgcctc gctcacggtg aaacatcccg actatgcact gctggccagc
     6661 cgcattgccg tcagcaatct gcacaagaat accatcaaaa gcttttcgtc gaccatgcga
     6721 ctcctgtacg aatgccagga tagcaagatc ggcaaggcga cgccgctact atctgaagag
     6781 gtctggcaga tcatatccga ccatgcagag ttgctggatt ctacgatcat ttatgaccgc
     6841 gattacggct tcgattattt cggcttcaag acgctcgaac ggtcctatct gctgaaagtc
     6901 gaaggcaaag tggtcgaacg tccccagcac ctgtatatgc gcgtggccat cggcatccat
     6961 catcacgata tcgaaggcgt catcaggacc taccacctga tgagcgagcg ctggttcacg
     7021 catgccactc ccaccctgtt caacgcaggg acaccaaaac cacagatgag ctcctgtttc
     7081 ctgctgacga tgaaggacga cagcatcgac ggcatctacg atacgctgaa acagaccgcg
     7141 aagatctccc aatccgcggg cggcatcggt ctgtccatcc acaacatccg cgctaccggc
     7201 acctatatcg gcggcaccaa tggaaccagt aacgggatca taccaatgtt acgggtcttt
     7261 aacgatacgg cgcgctatgt ggatcaggga ggcggcaaac gcaaaggcgc attcgccatc
     7321 tatctcgagc cctggcatgc cgatgtcttc gaattcctcg atcttcggaa aaatcacggc
     7381 aaggacgaga tgcgcgcccg cgatctcttc tatgcgctgt ggatccccga tctgttcatg
     7441 cagcgcgtgg agacaggagg taactggagc ctgttctgtc cgcatgaagc gccggggctg
     7501 cacgaatcct ggggcaaaga gttcgaggac ctgtataccc gttatgaaag cgaaggccgc
     7561 gcccgccgaa cggtcagggc gcaggatctc tggttcgcca ttttggatgc ccagatcgag
     7621 acgggtacgc cttatcttct gtataaggac gccgccaacc gcaaaagcaa ccagcagaac
     7681 ctcggcacca tccgaagctc caatctctgc acagagatca tcgaatatac atcgccggac
     7741 gaggtcgcgg tctgcaattt agcttcgctg gcgctgccgc ggtttgtgac gggcggcgtc
     7801 ttcgatcacc agaagttgta cgaggtgacc tatgaggtga cgaaaaatct caataagatc
     7861 atcgatcaca acttttaccc tatcgaggaa gcgcggaatt cgaatctgcg tcaccgtccc
     7921 ataggtctgg gtgtccaggg cctcgcggac gcattcatcc tgatgcggct gcccttcgag
     7981 tcggaaaggg cccgtcagct caataaagag atcttcgaga ccatctattt cgccgc
//
LOCUS       DASYSV010000162         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1184207, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000162 DASYSV010000000
VERSION     DASYSV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184207"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VGQ51_04985"
     CDS             complement(<1..392)
                     /locus_tag="VGQ51_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV9035947.1"
                     /translation="MTVRARITLLFTLLVVGILTLVCVSVYYFSYTNRIKDIQTRLAN
                     RAITTGRLLSQAGVFDRALIRKIDASTSVAMKDKVVEAYDNFDTRIYWYSDNPADTLR
                     TDKPVLNKARNSKESIYFTQGKKDAIAY"
     gene            complement(392..1090)
                     /locus_tag="VGQ51_04990"
     CDS             complement(392..1090)
                     /locus_tag="VGQ51_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9035948.1"
                     /translation="MAEERKILVIEDEKKIADTLKMGLAENGFEVDVAYDGKIGHQLL
                     ISRPFDLVILDINLPGMNGYELCKAIRYRNMNMLVIMLTSMSSLDDKIEGYDAGADDY
                     LVKPFEFRELLLKIRALLKRTPGQPVPVGNFLRAADLEMNLDNKEVHRAGRRIKLTAK
                     EFQLLEYLMRNKNRVVSRADLAINVWDVDFNTNTNVIDVYISYVRNKVDKDFEQKLIQ
                     THVGMGYILKTMSG"
     gene            1202..2038
                     /locus_tag="VGQ51_04995"
     CDS             1202..2038
                     /locus_tag="VGQ51_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV9035949.1"
                     /translation="MAIAFDKILIPVDFSLNTEIAVKKAAGLVGSGEAVLHLLHVMRP
                     GKHAVHQFKVWAVEKELEQWKDYIRENYPALRVKTQIMKGGSAQRMIIECAETLKPDL
                     IIIGRQNNRRRWAFARGISPDGIAKKSNCPVLTVKPGSLDSRTRVIVIPIRDFLPERK
                     LEWGVLLARRYKAQVHLLAIQENPEPKQWALPQVFLKAYHHLRENLHHPIEYSSIGQH
                     SPARAALDYAEMIMADMILVNPQTESGISGFACSRHISDLLGKDSKIQVLDVEPYDTK
                     KS"
     gene            2059..>2949
                     /locus_tag="VGQ51_05000"
     CDS             2059..>2949
                     /locus_tag="VGQ51_05000"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV9035950.1"
                     /translation="MKRLLAIFSSFLLMGGSKAQSIAGAQKLLYYGRYDGAAHQLHAL
                     LQTDPSNTEAWWLLTQVYVHDHRLAALQDTLQAMPAAVRQQPMALCAEGLLFLARKEK
                     DSASSCFNRALKATREKDPHVLLSVAIANETDHADSNDVKYAIDVLNKAIRRDKHNAE
                     LYVALGDAYRRLMDGANSYKAYSDALAQDSRSVDALYKLGKIFLTQNNTDMYLKYFDD
                     AVNLDSMYAPAWFELYYHYYFRDVNTAKRCLDHYIAASDPSVENDYLMTDLLYASRKY
                     SDAIEHAQGLITRQGKVSEPR"
BASE COUNT          711 a          801 c          754 g          683 t
ORIGIN      
        1 taataggcaa ttgcgtcctt ctttccctgt gtgaaataaa tgctttcctt gctgttccgc
       61 gctttgttga ggacgggttt gtcggtacgc agcgtatccg cggggttatc gctgtaccaa
      121 tagatccttg tgtcgaaatt atcgtaggcc tccacgacct tgtccttcat cgcgaccgag
      181 gtggaagcgt cgatcttccg gatcagcgcc cggtcgaaaa cgccggcctg gctcagcaaa
      241 cggccggtgg taatggccct gttggccaga cgggtctgga tatccttgat gcggttggta
      301 taggaaaaat agtaaacgga gacgcagacc agcgtcagta tgcctaccac cagcagggta
      361 aacagcagag ttatgcgggc tcttaccgtc atcagccgga cattgttttt agaatataac
      421 ccatgcccac gtgtgtctgg ataagcttct gctcgaaatc cttatcgacc ttgttccgga
      481 catagctgat atacacgtcg atcacattcg tattcgtatt gaagtcgaca tcccagacat
      541 tgatcgcgag atcggctctt gacaccaccc tgttcttgtt tcgcatgagg tattccagca
      601 gctggaattc tttggcggta agcttgatcc gtcgcccggc gcgatgcact tccttgttgt
      661 cgagattcat ttccagatcg gcggcgcgaa gaaaattgcc gacgggtacc ggctggcccg
      721 gggttcgttt gagcagcgcc ctgatcttaa gcagcagctc cctgaattca aacggcttga
      781 cgagataatc gtccgcaccc gcgtcataac cttcgatctt atcatccagc gagctcatgg
      841 aggtcagcat gatcaccagc atgttcatgt tccggtagcg aatagcttta cacagctcgt
      901 acccattcat gcccgggaga ttgatgtcga ggatgaccag gtcgaagggc cgggatatca
      961 acagctgatg gcctattttt ccatcgtagg ccacatccac ctcaaaacca ttttccgcca
     1021 gccccatttt cagggtgtcg gcgatctttt tctcatcctc gatgaccagt atctttcgtt
     1081 cttcggccat gtcacccaca atattacgga attacggcgc catccctcta ctctaatcca
     1141 attctaatgc cgctctaatt atttgttaat accagcggta tactttggga gaaataggaa
     1201 gatggccatc gcattcgata agatcctgat cccggtagat ttttcgctca ataccgagat
     1261 cgctgtgaaa aaggccgccg ggctggtagg gtcgggcgaa gccgtcctgc acctgttgca
     1321 tgtaatgcga ccgggaaaac atgccgttca tcaattcaag gtctgggcgg tggaaaaaga
     1381 actggagcaa tggaaggact acatccggga aaactatccc gcccttcgtg tgaagaccca
     1441 gatcatgaaa ggcgggtccg ctcagcggat gatcattgaa tgcgccgaaa cgctgaagcc
     1501 ggatctcatt attatcggca gacagaacaa tcgcaggcgc tgggcctttg ccaggggcat
     1561 ctccccggac ggcatcgcaa aaaaaagcaa ttgcccggta ctcacggtaa aacccggatc
     1621 gctcgacagc cgcaccaggg tcatcgtgat ccctatccgc gattttctgc ccgagcgcaa
     1681 actggaatgg ggggtattgc tcgccaggcg gtacaaggcg caggtacatc tactggcgat
     1741 ccaggaaaat cccgagccta agcaatgggc actgccgcag gtgttcctga aagcgtatca
     1801 ccaccttcgg gaaaatctgc atcatccgat agaatattcg tcgatcggcc agcatagtcc
     1861 agccagggcc gcactcgact acgccgagat gatcatggcg gatatgatcc tggtaaatcc
     1921 acagacggaa agcggtatct cggggttcgc ctgttcacgg catatcagcg atctgcttgg
     1981 caaggattcg aaaatacagg tgctggatgt agaaccatac gataccaaaa aaagctaaac
     2041 aacctatact gacgacttat gaaacgattg cttgccattt tctccagttt tttattgatg
     2101 ggtggctcca aggctcagtc gatagccggc gctcagaaat tgctgtatta cggacgctac
     2161 gacggagccg cccatcaatt acatgccctc ctgcaaaccg acccttccaa tacggaagct
     2221 tggtggcttc ttacgcaggt gtatgttcat gaccaccggc ttgccgcatt gcaggatacc
     2281 ctgcaggcca tgcccgccgc ggtaagacag caaccgatgg cgctttgcgc ggaaggtctg
     2341 ctcttcctgg ccagaaagga aaaggacagc gcctcgtcct gtttcaaccg tgccctgaaa
     2401 gcgaccaggg aaaaagaccc tcatgtcctg ctgtcggtgg ccatcgccaa tgaaacggat
     2461 cacgcagata gtaatgatgt gaaatatgcc atcgatgtat tgaataaggc catacgtcgc
     2521 gacaaacaca acgcggaact gtatgtcgct ctcggcgacg catatcgccg gctcatggac
     2581 ggcgccaatt cctataaggc ctattcggac gccctggccc aggacagccg gtcggtcgac
     2641 gcgctctaca agctggggaa gatcttcctg acgcagaaca atacggatat gtacctgaaa
     2701 tatttcgacg atgcggtcaa cctcgattcc atgtatgccc ccgcttggtt cgagctgtac
     2761 tatcattatt atttccgcga tgtcaatacg gcaaaaaggt gtctcgatca ctacatcgcc
     2821 gccagcgatc ccagcgtgga gaacgattat ttaatgaccg atctgctgta tgcttcccgg
     2881 aaatacagcg atgccatcga acacgcgcag ggactcatta cccgtcaggg gaaggtcagc
     2941 gaacccaga
//
LOCUS       DASYSV010000163         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1310723, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000163 DASYSV010000000
VERSION     DASYSV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310723"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VGQ51_05005"
     CDS             <1..898
                     /locus_tag="VGQ51_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035951.1"
                     /translation="HLRLPNGGYDLIVSYTGYETQVLRIGRDHRPTDTLQIRLKEQDK
                     SLEQAVVTGSAEVADGWEKYGQFFLDNFIGTTPNAAQCSLDNKEALKFYFYKKRNKLR
                     VKATNPLIVTNNALGYKIIYQLDSFVYDYNGNISSYTGYPLFDTLSGTPAQQATWKQN
                     RLYAYSGSRLHFIRSWYDSTLDYEGFVLELADSGNGNKMQRIQNPYDPQYYSRDSGDV
                     QIDINGRLRVSYTSQVPDRRYLIQNKFPLSTRFEISAIEIAGGFVIEENGYFYDQGDV
                     TNIGYWAWKKLAEVLPYDYVPE"
     gene            complement(957..1934)
                     /locus_tag="VGQ51_05010"
     CDS             complement(957..1934)
                     /locus_tag="VGQ51_05010"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEV9035952.1"
                     /translation="MRFPSPVSMHWLADFLGAELAGDKNGQATGINEIHKVEHGDLVF
                     VDHPKYYDTCLSSAASYIIINKAVEVPPGKAILIVDQPFEAYLRIVRHFRPFEPAVGM
                     ISDSATIGKDSYLYPNVFVGHHVTIGSHCIIHPNVTVLDHCVIGDNVVIQAGTVIGSD
                     AFYYNSRKDRAVWYKKMLSCGRVVIEDDVEIGAGCTIDRGVTGDTVIGRGSKLDNMIH
                     IGHDTVTGKNCLFAAQVGIAGAVGIGNGVILWGQVGVSKTLSIGDNAVVLAQSGVPAS
                     LETGKTYFGSPAEDAALKKRELVWIKRIPELWKKVMAGRPEEGRPKDNH"
     gene            2186..>2778
                     /locus_tag="VGQ51_05015"
     CDS             2186..>2778
                     /locus_tag="VGQ51_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEV9035953.1"
                     /translation="MRQYYVTYKPFQVLSQFTSQEKKRTLKDFFPVPADCYPVGRLDF
                     DSEGLLILTNDTALNHRLLDPRFAHEREYWVQVEGAFSAVALRQLQMGVTIAVDGKPY
                     RTRPCTVEIFPTTPPVPDRHPPIRYRARIPTSWIRMVLREGKNRQVRKMTAAVGFPTL
                     RLMRWRIGQLDLMGMVPGEMRILDERDIYSLLFAERN"
BASE COUNT          692 a          737 c          717 g          632 t
ORIGIN      
        1 tcatttgcga ctgcccaacg gcggttacga tcttatcgtt tcctatacgg gatatgaaac
       61 ccaggtcctg cgcatcggca gggaccatcg ccctaccgat acactgcaga taagactgaa
      121 ggaacaggat aagagcctgg agcaggcagt ggtcaccgga agcgccgagg tagccgacgg
      181 atgggaaaaa tacgggcaat tcttcctgga taattttatc ggcaccacgc ccaatgccgc
      241 tcaatgctcg ctggataaca aggaggccct gaaattctac ttttacaaaa agaggaacaa
      301 gcttcgcgtc aaagcgacga acccattgat cgtcaccaat aacgcgctgg gatataaaat
      361 aatttatcag ctggattcct tcgtctacga ttataacggc aatatcagca gctatacggg
      421 atatccgctg ttcgataccc tgtccggtac ccccgcgcag caggcgacgt ggaaacaaaa
      481 ccggctatat gcttattcgg gcagccgtct ccattttatc cgcagctggt acgatagcac
      541 tcttgactat gaaggattcg tgctggaact tgcagatagc ggcaacggca ataagatgca
      601 acggatccag aacccttatg accctcaata ctattcgagg gacagcgggg atgtgcagat
      661 cgatatcaac ggccggttaa gggtcagcta tacctcgcag gtacccgatc gaagatacct
      721 gatccagaat aagttcccat tgtctacccg gttcgagatc tccgctatcg agatcgcagg
      781 cgggttcgtc atcgaagaga acggctattt ctatgaccag ggggatgtca ccaatatcgg
      841 atactgggcc tggaagaagc tggcggaggt gctgccgtat gattatgtgc ctgaatgata
      901 aatcatcatc gtgctgcccg cataaggatt cgaccgcgcc ttcggcgcta ccggggctaa
      961 tgattgtcct tcggacggcc ttcctccggt cggccagcca tcaccttctt ccacaactcg
     1021 ggaatgcgtt tgatccaaac cagctctctt ttcttaagcg ctgcatcttc ggccggagag
     1081 ccaaaatagg tttttcccgt ttcaagagaa gcgggcaccc cgctttgggc aaggacgacg
     1141 gcattgtcgc cgatgctcag ggtcttgctg acacctacct gtccccagag gatgaccccg
     1201 ttgccgatcc cgactgcacc ggcaatgccg acctgcgcgg cgaagaggca atttttaccg
     1261 gtaacggtat catgaccgat atggatcata ttgtcgagct tgctccccct gccgatgacc
     1321 gtgtccccgg taacccctct gtcgatagta catcccgcgc cgatctcgac atcgtcttca
     1381 atgaccaccc tcccgcaact gagcatcttt ttgtaccaga cggcccggtc cttcctggaa
     1441 ttgtagtaga aagcatcgga cccgatgacc gttcctgcct ggatgaccac attgtcgcct
     1501 atgacacagt gatccaacac ggtgacatta ggatggatga tgcaatggct gccgatggta
     1561 acgtgatggc cgacaaacac attcggatac aggtaggaat ccttcccaat ggtggcggaa
     1621 tcgctgatca tcccgacggc cggctcgaag ggacgaaaat gacggacgat cctgagatag
     1681 gcttcgaatg gctgatcgac gatcaggatc gcctttcccg gaggcacttc cacggcttta
     1741 ttgatgatga tatagctggc ggcgctgctc agacaggtat catagtattt gggatgatcg
     1801 acgaagacaa gatcgccatg ttctacttta tggatctcgt taatgccggt agcctggcca
     1861 ttcttgtctc ctgccaactc agcgcccagg aaatcagcca gccagtgcat acttacggga
     1921 gaaggaaaac gcatagtgaa agaatttgcg cgtaaagtta ggccgacacc gcggtgaaac
     1981 acgcacattt tccacagccc aaaaaaaaat gtgaataaaa tttatgtgaa aattttttta
     2041 gataggaaaa aatattttta atattgtgta gggaaattta tttcccggta cttactaacc
     2101 catccatata ttgaaagccc ggtttcgcac cgggcttttt cttttgcaaa catggtcgcg
     2161 cgactcgcgg ccgattcgct gaaacatgcg ccagtactac gttacataca aaccattcca
     2221 ggtactctca caattcactt cacaggaaaa gaagcgtacg ctgaaggatt ttttccctgt
     2281 tccggcggat tgctatccgg tcggccggct ggatttcgac agtgaaggat tgctgatcct
     2341 gaccaatgac acggccttga atcaccggtt gctggatccg cggttcgcgc acgagcggga
     2401 atattgggta caggtggaag gcgccttctc cgctgtcgcc ctgcgccaac tgcagatggg
     2461 agtaacgatc gccgtcgacg gcaaacctta ccgaacccgc ccatgcacgg tcgaaatttt
     2521 tccaacgacg ccacccgtac cggacaggca tccacctatc cgttaccgtg cccggatacc
     2581 caccagctgg atcaggatgg tccttaggga gggaaagaac aggcaagtga gaaaaatgac
     2641 ggcggcggta ggcttcccta cgctgcgatt gatgcgatgg aggatcgggc aattggacct
     2701 catgggtatg gttccgggtg aaatgaggat cctcgacgaa agggacatct attcgttgct
     2761 gtttgccgaa aggaatca
//
LOCUS       DASYSV010000164         6632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1184585, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000164 DASYSV010000000
VERSION     DASYSV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184585"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VGQ51_05020"
     CDS             complement(<1..341)
                     /locus_tag="VGQ51_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEV9035954.1"
                     /translation="MRNNHEIDYKIYGEELQFVEVELDPGETVIGEPGGFMMMDDGIE
                     MQTLFGDGSASSNQGGLLGRLMNAGKRVLVGENLFMTAFTNIGTGKRKVSFAAPYTGK
                     IIPLDLQQLGGT"
     gene            complement(369..3956)
                     /locus_tag="VGQ51_05025"
     CDS             complement(369..3956)
                     /locus_tag="VGQ51_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9035955.1"
                     /translation="MSALENFTGKFKHFWPTTWSVKNRTAIYLVMLVFSGIGIFQFYT
                     LPKEQFPDIVIPTIYVSTIYTGNSPKDMENLVTRPIEKQIKAITGAKINKFTSTSQQD
                     YSAIMVEFETDVPTDVALQKVKDAVDKAKQDLPTDLTQQPTALEVSFSDLPIMYVNIS
                     GDYDQQRLKKFADDMKDRLEDLPQLNRVDIVGAPEREFQIDVDNYKMRASNITFSDID
                     QAVKAENRDISGGLLEVGNMKRNLQLKGQFHTTQDIESIILRSPQGGYPVYLKDIATI
                     KDTIKDRESYARLDGKNVVTLNIIKRSGENLIETSDTVRGIVNEMVKSLPRDLHVVVT
                     GDQSTQTRNSFNDLVNSIVIGFVLVLIILMFFMGVVNAFFVALSVPLSMFVAFTFLPG
                     AEFMIGGAVTLNFIVLFALLFGLGIIVDDAIVVIENTHRIFTQAKGRLTAPTSAMMAA
                     GEVFVPVLAGTLTTLAPFFPLLFWPGIIGKFMVYLPAMLIFTLTASLVVAFIMNPVFA
                     VDFMNHPEDGTPATRASIFKKPGLWIALGFGILLDLSGQRFLGNLLLFIVALVILNKF
                     VFDRMIHGFQNRFLPWIMLRYERLLRWALQGWRPAWLLVSTFLLLILSVVLIIVRNGG
                     VVFFPKGDPNQIFVYLKLPVGTNVDYTDSVTHVLERKVFSVLGMGDGSKKNPAVESVI
                     ANVAVGANDPNSGDQSTHPELGRIQVSFVEFKKRQGASTSMYLDSIRHVMKDIPGAEI
                     TVNQEQNGPPTEPPVNIEVASDNFDDLIKTAVSLKNYLDSIQTPGVEELKMDIDLTNP
                     EITLTVDRQRAMIEGINSATIGQELRTAVFGLESSKIKEGKDEYKIYIRNLESQRKSL
                     TDLLNMNITYRDIAAGGAIRNVPISSLVHVDYTSTLGSVKRKNQKRMITLTSNVLNGY
                     TPPAVNQDLAKSIAAFTHKPDDVSITQTGEGQQQAETTAFLGKALVIALATILVILVI
                     QFGSVAKSVIILNEILFSIIGVLLGFAITKMEISAVMTGLGIVGLAGIVVKNGILVIE
                     FTDELRARGMKTREAAIEAGKTRIIPVLLTAMAAILALVPLAVGFNINFVTLFSDLNP
                     QIYFGGDNAVFWKPLSWTIIFGLAFAFFMTLFMVPSMYLISERLRRPMRRHWGGKWVS
                     FLGIPPLTIVFLVLVFISMIRHRTEVARRRRRLAVAQKPADETWARSWF"
     gene            complement(4003..5154)
                     /locus_tag="VGQ51_05030"
     CDS             complement(4003..5154)
                     /locus_tag="VGQ51_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9035956.1"
                     /translation="MNKILTVVMASSLLFAACGSSSVNKDESLAAKKARLDSLNSQKG
                     KIEKQINSLQNEIDKLDTTSGKKEKAKLVTLTPMAPTSFTHYIDLQGNVEAVNMSYIS
                     PRGQGGVVTQLFVRQGDHVRQGQLLLKLDDAIQKQTLAADQTQLAYAKDLYQRRKNLW
                     DEKIGTEVDLVNAKNQVDQVEAKIKIDQQQLDFTNVYADIPGTVNTLKVKVGESFGPG
                     SQALQIVNTDNLKVVVQVPEIYQTRVRVGTPVKIAFPGLNNKEVTGTIAISGQVIDQN
                     NRAFQVEIHIPNSGDIRAYQVAIVKLQDYNATNVLTIPINTMQTDETGKYVMVGVDQN
                     GKLIAHKKSITVGQSYSDKIQVTGGLEVGDKLVTDGYKGLYEGQPIITQ"
     gene            complement(5181..>6632)
                     /locus_tag="VGQ51_05035"
     CDS             complement(5181..>6632)
                     /locus_tag="VGQ51_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9035957.1"
                     /translation="NFRMNKTYNQQRSIPRIDTQSQLRTIRRSPWRSLLSIALLVPVF
                     GLRAQQTPAASSSAMQAPQIHDFSVKEAIDYSSKNNVAVKNSLLDYQIQEQSNRATTS
                     AALPQVTGNLGLTDYIQIPTTLLPGEFLSPPQPGTYFPIKFGTKWNATGGVTLKQTLF
                     DGQVFVGLQARSASLEYYHQREEITQQMIRSNIYKIYYQLLIARSQIEQIDANITNQQ
                     ALLHNSTEMFKNGFAEQLDVDKATVQLANLQSEKIQTEFNVSNGYLALKVLMGMPVRD
                     SLHLTDTLTYDMLRDAALGDNYNYSDRHEFKLLEVGKQLNEFDVKRYKKMYIPTVSLT
                     AAYSQNQYVNEFDLAAKNSWFPTTYLGLNVSIPIFDGFNKDANIRQAKLRLQQTSNNM
                     DSLKIRIDNDVKEAQLRFSAALATLDYQKKNMELSERVYQQTRKKYEQGLGSNLEITN
                     ALTDQKTAQATFFNALYNAVIARVDYLTAIGKL"
BASE COUNT         1426 a         1668 c         1855 g         1683 t
ORIGIN      
        1 gtaccgccca gttgctgcag gtcaagagga atgatcttcc cggtatatgg cgcggcaaag
       61 ctgacctttc tcttgccagt gccgatattg gtaaatgcgg tcatgaagag attttctccg
      121 accaggacac gtttacccgc attcatgagc cggcctagga ggccaccctg attggaagag
      181 gcggagccat ccccgaaaag cgtctgcatc tcgatgccgt catccatcat catgaagcct
      241 cccggttccc cgatcacggt ttcgccgggg tcgagctcta cttctacgaa ctggagttct
      301 tcaccataga tcttatagtc gatttcgtgg ttattgcgca tataataatc gtttaggtta
      361 tttgaatttt agaaccagct acgggcccag gtttcatcag cgggtttttg agcgacggct
      421 aacctgcgtc ttcttctagc gacttccgtt ctatgccgga tcatggagat gaataccagg
      481 accaggaaca cgatggtcaa tgggggtatt cccaggaatg atacccattt gcctccccag
      541 tgacgacgca tcgggcggcg aagtctttcc gagatcaggt acatgctcgg caccatgaac
      601 agggtcatga agaaggcgaa tgccagaccg aagataatgg tccaggacaa gggtttccag
      661 aacaccgcgt tatcgcctcc gaaatagatc tgcggattca gatcggagaa cagcgtgacg
      721 aaattgatat tgaaccccac ggccagcggt accagggcaa gaatggcggc catggccgtc
      781 agcaggacgg gaatgatacg ggtcttaccg gcttcgatgg cggcttcgcg ggttttcatg
      841 ccgcgggcgc gcagctcatc cgtaaattcg atcaccagga taccgttctt gacgacgatg
      901 ccggccaggc ctacgatacc aagaccggtc atgaccgccg agatctccat tttggtgatc
      961 gcgaaaccca gcaggacgcc gatgatgctg aagaggatct cattcaggat gatcaccgac
     1021 ttggcgaccg agccgaattg gatgaccagg atgacgagga tcgttgccag cgcgatgacc
     1081 agggctttgc cgagaaaggc ggtcgtctcc gcctgctgtt gtccctcacc cgtctgagtg
     1141 atagagacat catccggttt gtgcgtgaag gctgcgatgg atttcgcgag atcctggttg
     1201 accgcaggtg gagtataccc attgagtaca ttcgaggtca gggtgatcat ccttttctgg
     1261 ttctttcgtt tgacgctgcc cagtgtgctg gtatagtcga catggaccag cgaactgata
     1321 ggaacattcc ggatggctcc gcctgcggcg atatcccggt aggtgatgtt catattgagc
     1381 aggtcggtca gacttttacg ctgcgattcc aggttgcgga tatagatctt atactcgtcc
     1441 ttcccttctt tgatcttcga agattcgaga ccgaacacgg cggtacggag ctcctgcccg
     1501 atagtggcgg agttgatccc ctcgatcatt gcgcgctgcc ggtcgacagt cagggtgatc
     1561 tccgggttgg tgagatcgat atccattttc agctcttcca cgccgggtgt ttgtatcgag
     1621 tcgagatagt tcttcagcga gacagccgtt ttgatgagat cgtcgaaatt gtcgcttgca
     1681 acttcaatat tgacgggagg ctcggtaggc ggaccgttct gttcctggtt gacggtgatc
     1741 tctgcgccgg ggatgtcttt catcacatgg cggatagaat cgagatacat ggaagtcgat
     1801 gccccctgac gtttcttgaa ttctacaaag gatacctgga tgcggccgag ctcggggtgg
     1861 gtactctggt cgccgctatt ggggtcgttg gcgccgaccg caacgttggc aataacgctt
     1921 tcgacggcgg ggttcttttt tgaaccgtcg cccataccga gcacggaaaa taccttgcgt
     1981 tcgagcacgt gggtgaccga gtcggtataa tctacattgg tgccgacagg cagtttgaga
     2041 tatacgaata tctggtttgg atcacccttg gggaagaata cgactccgcc attgcgcaca
     2101 atgatcagca ccaccgacaa gatcaacagg agaaaggtgc tgaccaggag ccatgcaggc
     2161 cgccagccct gaagggccca ccttagcagt cgttcatacc ggagcatgat ccagggcaga
     2221 aagcggttct ggaaaccgtg gatcatccgg tcgaaaacga atttattgag aatgacgagg
     2281 gcgacaatga acaatagcaa attcccgagg aaacgctggc cggaaagatc gagtaaaatg
     2341 ccgaaaccga gcgcgatcca caatccgggt tttttgaaaa tggaggcacg ggtggccggc
     2401 gtcccgtctt cgggatggtt catgaaatcc actgcaaaca ccgggttcat aatgaacgcc
     2461 acgacaagcg acgctgtcag ggtgaagatc agcatggccg gcagatagac catgaatttc
     2521 ccaatgatac cgggccagaa cagcaggggg aagaaaggcg ccagggttgt cagggtacct
     2581 gccaacacgg ggacgaagac ctcacctgcc gccatcatgg cggaggtcgg cgccgtcaac
     2641 ctgcccttcg cctgggtgaa gatgcggtgt gtattttcga tgaccacgat ggcatcgtcg
     2701 acgatgatac ccagcccgaa gaggagcgcg aagagcacga taaagttaag ggtgaccgcg
     2761 ccgccgatca tgaattcggc gccgggtaaa aaggtaaagg cgacgaacat gctcaaagga
     2821 acggacagcg ccacgaagaa ggcgttgacc actcccataa agaacatcag gatgatcagc
     2881 accagtacga atccgataac gatcgaattg accaggtcat tgaaagaatt cctggtttgg
     2941 gtgctttgat cgccggtcac gacgacatgc agatcccgcg gcaggctctt caccatttcg
     3001 ttgacgatac cgcggaccgt atccgaggtc tcgatcaggt tctcacccga acgcttgata
     3061 atattgagtg tcacgacgtt cttcccgtcg agacgggcat agctctcccg gtctttgatc
     3121 gtatccttga tggtagcgat atctttcaga tatacgggat agccgccctg cgggctgcga
     3181 aggattatcg attcgatatc ctgggtcgta tggaactgtc ccttcaactg gagattgcgt
     3241 ttcatatttc ccacttcgag gaggccgccg gagatatcgc ggttctccgc tttcaccgcc
     3301 tggtcgatat cgctgaaggt tatgttggac gcgcgcatct tgtagttgtc gacgtcgatc
     3361 tggaattcgc gttcgggggc gccgacgatg tccaccctgt tcaactgcgg aaggtcttcc
     3421 agcctgtcct tcatatcgtc ggcaaatttt ttcagccttt gctgatcgta atcgccgctg
     3481 atattgacat acatgatcgg caggtctgaa aaactgacct caagggctgt cggttgttgt
     3541 gtcagatcgg tcgggaggtc ttgtttggcc ttgtcgacgg catccttcac cttttgcaac
     3601 gctacatcgg tcgggacgtc ggtttcgaat tcaaccatga tggccgaata gtcctgttgc
     3661 gaggtgctgg tgaatttatt gatcttggct cccgtaatgg ccttgatctg tttttcgatg
     3721 ggccgcgtca cgaggttctc catgtctttc ggggagttac ccgtatagat ggtggagaca
     3781 tagatcgtcg ggatgacgat atccgggaac tgctctttcg ggagcgtata aaactggaag
     3841 atgccgatac cactgaagac cagcattacc aggtagatgg ccgttcgatt tttgacactc
     3901 caggtggtgg gccagaagtg cttgaatttg ccggtaaaat tttctaaagc gctcataact
     3961 tttttttcag atgaatgaag gattgacggt tggtacgtcg gcctattggg ttattatcgg
     4021 ttgtccttcg tacaggccct tgtatccgtc ggtgaccagc ttgtcgccga cctccagacc
     4081 gcctgtgacc tgtatcttgt cggagtagga ttggcctact gtgatcgact tcttatgcgc
     4141 gatgagcttg ccgttctgat ccacgcctac catcacatat ttaccggtct cgtcggtctg
     4201 catggtattg atgggtatgg tgagcacatt ggtcgcgtta taatcttgca gcttgacgat
     4261 cgccacctgg taggcgcgaa tatcgccgct gttggggata tggatctcta cctggaaggc
     4321 gcggttattc tgatcgataa cctgtccgga gatggctatg gtccccgtga cctccttatt
     4381 gttgagaccg gggaacgcga tctttaccgg agtacccacc cgaacgcggg tctggtatat
     4441 ctccggcacc tgaacgacga ccttgagatt atcggtattg acgatctgga gcgcctggct
     4501 tcccgggccg aagctttcgc cgacctttac tttaagggta ttcaccgttc cgggaatgtc
     4561 ggcatagaca ttggtgaagt ccaactgttg ctgatcgatc ttgatctttg cctctacctg
     4621 gtcaacctgg ttctttgcgt tgaccaggtc tacttccgtc cctatcttct cgtcccatag
     4681 gttcttgcgg cgctgataaa ggtctttggc atatgcaagt tgtgtctggt cggcagccag
     4741 ggtttgcttt tggatggcgt cgtcgagctt aagcagaagc tgaccctgac ggacatggtc
     4801 gccctgtctg acgaagagct gggtcacgac accgccctgc ccgcggggtg agatatagga
     4861 catattgacg gcttctacgt taccctgcag atcgatatag tgtgtaaagg aggttggcgc
     4921 catgggggtc agcgtgacca acttggcctt ttccttcttg ccggatgtcg tatcgagctt
     4981 gtcgatctcg ttctgaagag agtttatctg tttttcgatc tttccctttt gggagttgag
     5041 actgtcgaga cgggcttttt tcgccgcgag cgattcgtcc ttgttgaccg aactgctgcc
     5101 gcaggcggca aacaggagag aagaagccat gacaacagtt aatatcttat tcatacgatg
     5161 tgctttagaa ttttttacgt ttataatttt ccaatggcgg tgaggtaatc cactcttgct
     5221 atgacagcgt tgtacaaggc gttgaagaag gtggcttgcg ccgttttctg atcggtcagg
     5281 gcattcgtga tctcgagatt ggagccaaga ccctgctcat atttctttct tgtctgttgg
     5341 taaaccctct cggatagctc catattcttc ttctggtaat ccagggtcgc cagcgcggcc
     5401 gaaaagcgaa gctgggcttc cttcacgtcg ttgtcgatcc ggatcttcag cgagtccata
     5461 ttgttcgagg tctgctgcag gcgcagcttc gcctgcctga tattcgcatc tttgttgaaa
     5521 ccgtcgaata tcgggatgct gacgttcaga cccagataag tggtggggaa ccagctgttc
     5581 ttcgccgcca gatcgaactc attgacgtac tgattctggg aataggcggc ggtcagcgac
     5641 acggtcggta tatacatctt tttataccgt ttgacatcga attcgttcag ctgtttgccg
     5701 acttccagca acttgaattc atgacggtcg gaatagttgt agttatcccc caatgcggca
     5761 tcccgaagca tatcgtacgt aagggtatcg gtgagatgca gtgaatcccg gaccggcatg
     5821 cccatcagta ccttcaaggc cagatagcca ttggagacat tgaactccgt ctggatcttc
     5881 tcagactgca gattggccag ctgaactgtc gccttatcga catccagctg ttcggcaaaa
     5941 ccgttcttga acatttccgt tgaattgtgc agcagggcct gctggttggt gatattggcg
     6001 tctatctgct ctatctgcga tctggcgatc agcagctggt aatatatctt atagatattg
     6061 gaccggatca tctgttgggt gatctcctcc cgctggtgat aatattcgag cgaagcgctg
     6121 cgcgcctgaa ggcctacgaa cacctgaccg tcgaacaatg tctgcttcag ggtgacgccg
     6181 cccgtagcat tccacttcgt cccgaatttg atcgggaaat aggtgccggg ctgtggcggg
     6241 ctcaggaact ctcccggtag gagcgtcgtc ggtatctgga tataatccgt gagtccgaga
     6301 ttgccggtca cctgcggaag cgccgccgaa gtcgtcgcgc gattgctttg ctcctgtatc
     6361 tggtaatcca ataaggaatt cttgaccgcc acattgttct tcgatgaata atcgatcgct
     6421 tctttcaccg agaaatcatg gatctgcggc gcctgcatcg cggaagaaga ggccgccggg
     6481 gtttgctgag cacggaggcc gaataccggc accagtaaag ctatggacag caaactgcgc
     6541 caggggcttc gccggatagt tcgcagttga ctttgcgtgt cgatccgcgg gatgcttcgt
     6601 tgctgattat acgttttgtt catgcgaaag tt
//
LOCUS       DASYSV010000165         7773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2462022, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000165 DASYSV010000000
VERSION     DASYSV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462022"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1746)
                     /gene="groL"
                     /locus_tag="VGQ51_05040"
     CDS             complement(109..1746)
                     /gene="groL"
                     /locus_tag="VGQ51_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583723.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV9035958.1"
                     /translation="MAKQLFFEIEARNKMKKGVDTLANAVKVTLGPKGRNVVLEKKFG
                     APAITKDGVTVAKEIELEDPIENMGAQMVKEVASKTADIAGDGTTTATVLAQSIISEG
                     LRNVAAGANPMDLKRGIDKAVEKVVESLSAQSKTIGSDKNKIEQVASVSANNDSAIGK
                     LIAEAFGKVGKEGVITVEEAKGTDTTVEVVEGMQFDRGYISPYFVTNSEKMEAELQNP
                     YILIYDKKISAMKDILHILEKVAQGGRPLLIIAEDLEGEALATLVVNKLRGTIKVAAV
                     KAPGFGDRRKEMLQDIAILTKGIVISEEQGYKLEGADLSYLGTAASVTIDKDNTTVVG
                     GKGKKEDITARVNQIKAQIESTTSDYDKEKLQERLAKLAGGVAVLYVGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVPGGGIAYIRAVESLEKLKGANEDETTGIAIVKRALEEP
                     LRQIVANCGVEGSIVIQKVKEGQADYGFNARTEKYENLYRAGVIDPTKVARIALENAA
                     SIAGMLLTTECVVADKPKKEEAHAHPGGAPGMGGMDY"
     gene            complement(1775..2056)
                     /locus_tag="VGQ51_05045"
     CDS             complement(1775..2056)
                     /locus_tag="VGQ51_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV9035959.1"
                     /translation="MGKKLNVTPLSDRVIVKPAPAEEKTAGGIIIPDTAKEKPQRGTV
                     VAAGPGKKDEPVTVKVGDTVLYGKYAGTEIQIEGDDFLIMRESDILAIV"
     gene            2331..3257
                     /gene="nusB"
                     /locus_tag="VGQ51_05050"
     CDS             2331..3257
                     /gene="nusB"
                     /locus_tag="VGQ51_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV9035960.1"
                     /translation="MISRRNIRVKVMQTLYTLEAQEQTIDSPDAVKILQKHFDQSRQL
                     LSYLLYIIIEIARYAETDSRLRASKHLPSAEDLNVNIKLAGNEFLWKMLGDESLKKAL
                     AADKPHLQENKDLIRKLYHGLVATEEYQRYIRQQSRDRKTERDILELIFGTIVLPNDS
                     FLFHIEELFSNWDDDGEMISQIVMSYIAKPGSVDFQEIISKVKWDFARILLQTVIDKK
                     EVVMDLIRPRLKNWDPERIATLDMILMQMGVVEFLYFETIPPKVTINEYIDLAKDYST
                     PQSGQFVNGILDNIHKELVHEDKMHKVDFRTN"
     gene            3284..3727
                     /locus_tag="VGQ51_05055"
     CDS             3284..3727
                     /locus_tag="VGQ51_05055"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEV9035961.1"
                     /translation="MRTFLACCLFTVLIASSVASCHSDSTSVAASAAAMADSTRFTTI
                     QWLDSSYRDFGRIPEGQKLDVSFRFRNTGNMPLVIARVQPSCGCTIAEQPKAPIAPGA
                     EGQIRATFNSEGRPGVNHKTLFVSSNTKGKQTWSLMFSVVVDKKS"
     gene            3751..4095
                     /gene="yajC"
                     /locus_tag="VGQ51_05060"
     CDS             3751..4095
                     /gene="yajC"
                     /locus_tag="VGQ51_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV9035962.1"
                     /translation="MTHSLLFVLLQAGAPSGGGSLQLILLGGMILVFWLFMIRPQAKK
                     AKLAKTFQEGMQKGDKIVTIAGIHGTVNKINEDGTLMIETSPGSYLKIEKSAISMEWT
                     QNLNKATAAATK"
     gene            4142..4750
                     /gene="coaE"
                     /locus_tag="VGQ51_05065"
     CDS             4142..4750
                     /gene="coaE"
                     /locus_tag="VGQ51_05065"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793821.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV9035963.1"
                     /translation="MLRVGVTGGIGSGKSTVAAIFEVIGIPVSYADAEAKRLMNEDSG
                     LRESIRLHFGDEAYVNGQLDRKYLAAKVFGDPAQLQLLNSLVHPVTIREGRRWMQYHS
                     TLDHPYAIREAALLFESAAADELDFIIGIYAPPTLRIHRAMQRDNVTREEVLRRMNNQ
                     IDEEIKMRLCDAVIYNDENHPVIQQVLDLHRQLITLASRERP"
     gene            complement(4722..5861)
                     /locus_tag="VGQ51_05070"
     CDS             complement(4722..5861)
                     /locus_tag="VGQ51_05070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV9035964.1"
                     /translation="MAKLGRELRAKGIDVIDLSLGEPDFDTPEHIKNAAKKAIDDNWS
                     HYTPVAGYADLREAVCVKFKRDNGLDYKPENIVVSTGAKQSLANVILALVDEGDEVII
                     PTPYWVTYSELVKIARGKVAEVHTTLESGYKITPAQLEKAITPKTKVFLFSSPCNPSG
                     AVYSKSELAALAEVFRKHPEVVIISDEIYEYINFIGKNESIAQWPDLKDQVVIVNGLS
                     KGFAMTGWRLGYIAANVEIARACEKLQGQFTSGTNSIAQKSAVVALTADLAPSMDMVK
                     EFTARRERTLQLVKSVPGLKCFEPQGAFYIFPDVSYYYGKSDGTTTISNSADFSMYLL
                     NTAHVSSVMGDAFGEPACVRFSFANSMGNIEKAWGRIKDALAKLK"
     gene            5988..>7773
                     /locus_tag="VGQ51_05075"
     CDS             5988..>7773
                     /locus_tag="VGQ51_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HEV9035965.1"
                     /translation="MRPLVSLFAALLIVISVYQLSFTYFVNKHESAMQAKAKTAVSRL
                     YPQSAAQKYPGDKEAQALYQDTIDEVYNARLARLLDSTKETKITPWGSTYQKSKESEL
                     LLGLDLQGGMSVTMDIQLDKLLIGLANNPRDPNVLKAIAMAQQKKMNSSTNLIDLFAE
                     SYKEINPTAKLAPLFSNINRNKIGYDASDATVLSWLHDEANAAMSETYRVLDNRINQF
                     GVAQPNINLDQAKGIITVELAGAKDPERIRKYLQSSANLQFWEVYSWPDLANDFVAAD
                     KALQGYLNGAKADTAAANRGVANTADSTGANKVAAKDTTKKDTTTQLANANPLFHVLA
                     PAESRGQNGQTPSYSFVGRSMLKDTGTVNNYLNNPIVRNNFPQDLKFLWGKQDLDENG
                     KKLPYLELYAIKTLPGSDKARLQGTIVDDASQDFDPITSDVVVEMSMNKQAAKIWGDM
                     TTKNVNKPIAIVLDDIVYSAPTVNEPITGGSSRITMGRGRGSSDQTITEAKDLANILK
                     SGQLDAPAKIVQEQVVGPTLGQEAVHGGMMAFAISFVVIFALMLIYYNTGGWVANCAL
                     ILNLLFTIGVLSSLGATLTAPGIAGLVLT"
BASE COUNT         1846 a         2290 c         1880 g         1757 t
ORIGIN      
        1 tatcggcatg aaatggtagg acgggcgccg aaggccccgt cgccgccagg caatcataaa
       61 acaaaaaacc ccgcgaaagc ggggtcttgt attcttggag aacagaattt aataatccat
      121 tccacccata cccggtgcac cgccgggatg agcatgcgct tcttctttct tgggcttgtc
      181 ggcaaccacg cattcggtgg tcagcaacat tcctgcaatg gaagctgcat tctcgagcgc
      241 gatacgggcg accttggtgg gatcgataac gccggctctg tacaggttct catatttttc
      301 cgtccgggca ttgaaaccgt aatcggcttg accctctttt actttctgta tgacgatgct
      361 gccttccaca ccgcagttgg caacgatttg tcgcagcggt tcttccagcg ccctcttgac
      421 gatagcgatt ccggtggttt cgtcttcgtt ggcgcctttc agcttttcga gcgattcgac
      481 agcgcggata tatgcgatac cgccgccggg cacgatgcct tcttcaacgg ctgcacgggt
      541 tgcatgcagg gcgtcgtcga cgcggtcttt cttctctttc atctcgactt ccgtggcagc
      601 acctacgtac aatacggcta caccccccgc taacttggca agacgctcct gcaatttctc
      661 cttatcgtaa tccgaagtgg tggactcgat ctgcgccttg atctggttga cgcgggcggt
      721 gatatcttct tttttgccct taccgccaac gacggtagta ttgtccttat cgatagtgac
      781 cgaagcagcc gtaccgagat agctcagatc ggcgccttcg agtttataac cctgctcttc
      841 gctgatcacg atacccttgg tgaggatcgc gatgtcctga agcatttcct tgcggcgatc
      901 gccgaaaccg ggagccttta cagcggcaac cttgatggtt ccacgcagtt tgttgaccac
      961 cagcgtagcc agcgcctcgc cttccaggtc ttctgctatg atgagcagcg gacggccgcc
     1021 ctgtgctact ttttccagga tatgaaggat gtccttcatt gccgagatct tcttatcgta
     1081 gataagaata tagggatttt gcaattcagc ttccatcttc tcgctattcg taacgaaata
     1141 gggagagata taaccgcggt cgaactgcat gccttctacg acttctacag tcgtatcggt
     1201 acctttcgct tcttccacgg tgatgacgcc ttccttacct actttaccga aagcctctgc
     1261 gataagcttg ccgatagccg aatcattgtt ggccgatacg gaagccacct gctcgatctt
     1321 gttcttatcg ctaccgatgg tcttcgactg tgcgctcagg ctttcgacta ctttttcaac
     1381 cgccttgtcg ataccgcgct tcaggtccat cgggtttgcg cctgcggcta cattcctgag
     1441 accttcgctg atgatggact gggccagaac cgtagcggtg gtggttccgt caccggcgat
     1501 atcggccgtt ttggaagcca cttctttcac catctgcgca cccatgttct cgatggggtc
     1561 ctccagttcg atctccttgg ccacggtaac accgtccttg gtgatcgcgg gagcgccgaa
     1621 tttcttttcc aatacgacat tgcgtccttt ggggccgagt gtcaccttga cggcgttggc
     1681 cagcgtatcg acgccctttt tcattttgtt gcgggcttcg atttcgaaga agagttgttt
     1741 tgccataatt atttcagttg agctattttc aaaattaaac gattgctaaa atgtcagatt
     1801 cacgcatgat cagaaaatcg tcaccttcga tctggatctc ggtgcctgca tacttgccat
     1861 acaataccgt atccccgact ttcacggtta ccggctcgtc cttcttgcca ggaccggcgg
     1921 ctactaccgt accacgctgc ggcttctctt ttgccgtatc gggaatgatg ataccgcctg
     1981 ccgtcttttc ctcggcggga gccggcttca caattaccct gtcagataag ggggtaacgt
     2041 ttaacttttt gcccatagtt gtattgattt tgttgttttg atgaaagatg agtctcctgc
     2101 ggcgcctccg cctcctcgca ggtgtgcaaa ggtacacgaa atttatacca aacccggtcg
     2161 caatgccttt tttgcagttt ttggctgacc ggaaccccat ccgggccact ccaggcagcc
     2221 cgcttcgtgt cattttttca gggccatatc cggctgtttg tcagtgggtt tcacccaatg
     2281 gtcaaattac cctatatttg cagcccaaag aataaagttc attcaaataa atgatcagta
     2341 gaagaaatat ccgggtaaag gtgatgcaaa ccctttatac gctggaagcg caggaacaga
     2401 ccatagattc gcccgatgcc gtcaagatcc tgcaaaagca ctttgatcag tccaggcaat
     2461 tattatccta tctcctttat atcatcatcg agatcgcccg ctacgctgag acagattccc
     2521 gcctcagggc ctccaaacac cttccaagcg cggaagatct aaatgtcaat atcaagctcg
     2581 caggcaacga gttcctctgg aagatgctcg gggacgaatc attgaaaaag gcgctggcag
     2641 ccgataaacc ccacctgcag gaaaataagg acctcatccg caaattatat cacggactcg
     2701 tggccaccga agaatatcag cgctatatcc gtcaacagag ccgcgaccgc aaaaccgaac
     2761 gcgacatcct tgaattgatc ttcggcacca tcgtcctgcc caacgattcc tttctctttc
     2821 atatagaaga gcttttctcc aactgggacg acgacgggga aatgatcagc cagatagtaa
     2881 tgagctatat cgccaaaccc gggtcggtcg atttccagga gatcatcagc aaggtaaaat
     2941 gggacttcgc ccggatcctc ctgcaaaccg tcatcgacaa aaaagaggtg gtgatggacc
     3001 tcatccgccc ccggctcaag aactgggacc ccgaacggat cgcgacgctc gacatgatcc
     3061 tgatgcagat gggtgtggtg gaattcctct atttcgagac catcccgccc aaagtgacca
     3121 tcaacgaata tatcgacctg gccaaggact atagcacgcc gcaaagcgga cagttcgtca
     3181 atggtatcct ggacaatatc cataaagagc tggtccacga agacaagatg cataaggtcg
     3241 atttcaggac gaattagtac gtttgcaaaa aaataccaac cgcatgcgca cctttttggc
     3301 ctgttgcctg tttaccgtac tgatcgcttc gtcggttgcc tcctgtcatt ccgacagcac
     3361 gtcggtcgcg gcttccgctg cggctatggc cgattccacc cggttcacca ccatccaatg
     3421 gctggactcc tcctacaggg actttggcag gatccccgaa ggacagaagc tggacgtctc
     3481 cttcagattc aggaataccg gaaacatgcc gctcgtgatc gcccgcgtcc agcccagctg
     3541 cggatgcacc atcgccgagc aacccaaagc ccctatcgca ccgggcgccg aaggacagat
     3601 ccgtgcaaca ttcaacagcg agggccgtcc gggtgttaat cataagacct tattcgtcag
     3661 ctccaacacg aagggcaaac agacctggtc gctcatgttc tccgtggtgg tcgacaagaa
     3721 atcataattc aatacaaaca ataatcaaca atgacgcatt ccttactatt cgttttatta
     3781 caggccggtg ccccctcagg cggcggttcg cttcaactga tcctcctcgg cggtatgatc
     3841 ctcgtgttct ggctcttcat gatacgcccc caggccaaga aagccaaatt ggctaagacg
     3901 ttccaggagg gtatgcagaa aggagacaag atcgtgacca tcgccggcat ccacggaacc
     3961 gtgaataaga tcaacgaaga cggcaccctc atgatcgaga cgagcccggg cagctatctt
     4021 aagatagaaa aaagcgccat ctccatggaa tggacgcaaa acctgaataa ggctactgcc
     4081 gccgctacca aataaacagt aaagcccggt catcccgggc tttatcattg acccattgcc
     4141 tatgttgcga gtcggtgtaa caggcggcat cggttccggg aaatccacgg tagccgccat
     4201 tttcgaagtc atcggtatcc ccgtctccta tgcagacgcg gaagccaaac gtctcatgaa
     4261 tgaagactcc ggccttcggg aatccatccg ccttcacttc ggcgacgagg cctatgtcaa
     4321 cggacagctc gaccggaaat acctggccgc aaaagtcttc ggcgaccctg cacagcttca
     4381 gctgctcaac agtctcgtac accccgtgac catccgcgag ggccggagat ggatgcagta
     4441 ccactcgacc ctggatcacc cgtacgccat ccgcgaagcc gccctccttt tcgagagcgc
     4501 ggccgcagac gagctcgatt tcatcatcgg tatatatgcc cctcctaccc tgcgcatcca
     4561 ccgcgccatg cagcgcgaca acgtcacgcg cgaagaagtg ctccggcgaa tgaacaacca
     4621 gattgacgaa gaaattaaaa tgagactttg cgacgcggtc atctacaacg acgagaacca
     4681 cccggtgata cagcaggtgc tcgacctgca ccggcagctc attactttag cttcgcgaga
     4741 gcgtccttga tccttcccca cgccttttcg atattcccca tgctgttggc aaaagaaaat
     4801 cgcacgcagg ccggctcgcc gaacgcatct cccatcacgg aagacacatg cgccgtattc
     4861 aaaagataca tggaaaaatc agcggagttc gatatcgtcg tcgtaccatc cgatttgccg
     4921 tagtaatagg acacatccgg gaaaatataa aaagcgccct gcggctcgaa acatttcaga
     4981 cccggtacgg acttcaccag ctgtagcgtc cgctcacgac gcgccgtaaa ctcctttacc
     5041 atgtccatcg acggcgccag atcggccgtc aacgccacaa ccgccgattt ctgggcgatg
     5101 ctattcgtac cgctggtgaa ctgaccctgc aatttctcac aggccctggc gatctccaca
     5161 ttggctgcga tataacccaa ccgccatcct gtcatggcaa aacccttgct cagaccgttg
     5221 acgatgacca cctggtcctt cagatccggc cactgcgcta tactttcatt cttgccgata
     5281 aaattgatat attcatatat ttcgtccgaa ataatgacca cttcggggtg cttccggaat
     5341 acttccgcca acgccgccag ctcactcttg ctgtaaacgg ctcccgaggg gttacaaggc
     5401 gatgagaaca gaaatacctt cgttttcgga gtgatcgcct tttccagctg cgccggcgtg
     5461 atcttataac cgctttccag cgtggtatgc acttccgcta ccttcccacg cgctatcttt
     5521 accaattcgg aataggtgac ccagtacggc gtcggtatga tcacctcgtc gccttcatcc
     5581 accagcgcaa ggatgacatt ggccagactc tgcttagcgc cggtcgaaac gacgatgttc
     5641 tcaggcttgt aatccagtcc gttatcccgt ttgaatttga cgcacacggc ttcgcgcaga
     5701 tcggcatagc ccgcaaccgg tgtatagtgc gaccagttgt cgtcgatggc tttcttcgcg
     5761 gcattcttga tatgctctgg ggtgtcaaaa tcaggctcgc caagggagag atcgatgacg
     5821 tcgatgcctt ttgcacgtag ttcacgtcct aacttggcca tttttaacgt ttccggctcg
     5881 ctgaagcggg agagaatgga ggataatcgc ataaattgat aaattgcggc gcgaaggtag
     5941 gaaaaaacct atatttgccg ttcctaaaat tcaaacgtca ttttacaatg agaccactgg
     6001 taagtctttt tgctgcacta ctgatcgtta tctccgtata ccagctttcc ttcacctatt
     6061 tcgtgaacaa gcacgaatcg gcgatgcagg caaaggccaa aacggccgtt tcccggctat
     6121 acccccaatc cgctgctcaa aaataccccg gcgacaaaga ggcccaggct ctctaccagg
     6181 acactatcga cgaggtctac aatgcccgcc tcgccaggct gctggacagc acgaaagaaa
     6241 ccaagatcac tccatggggc tccacctatc aaaagtccaa ggaaagcgag ttgctgctgg
     6301 gtctcgatct gcagggaggc atgagcgtta caatggatat ccagctggac aaattgctca
     6361 ttggactggc caacaacccg cgtgacccga atgtgctgaa ggcgatcgcc atggcccagc
     6421 aaaaaaagat gaacagcagc accaacctca tcgatctgtt cgcggaatcc tataaagaga
     6481 tcaatccgac cgcgaagctt gctccgctgt tctccaatat caaccgtaac aagatcggct
     6541 acgacgcttc cgatgcgacg gtgctctcct ggctgcatga cgaagccaac gccgcgatga
     6601 gcgagaccta cagggtcctc gacaatcgta tcaaccagtt cggcgtagcc caaccgaata
     6661 tcaatctcga ccaggccaag ggtatcatca ccgtcgagct ggccggcgcc aaagaccccg
     6721 agcggatccg caaatacctc cagtccagcg ccaacctgca gttctgggaa gtgtacagct
     6781 ggcccgacct cgcgaatgat tttgtcgctg cagacaaggc gctccaggga tatctcaacg
     6841 gagcaaaggc cgatacggct gccgcgaatc gaggcgtcgc gaacacagcc gatagtaccg
     6901 gcgccaacaa agtcgcagcg aaggacacga cgaaaaaaga taccacgacc caactggcca
     6961 acgccaatcc gctattccac gtactggctc cggccgaaag ccggggacag aatggtcaga
     7021 cgccgagcta ctctttcgtc ggccgctcca tgctgaagga caccggcacc gtcaacaact
     7081 atctcaataa ccccatcgtc aggaacaatt tcccgcagga cctcaaattc ctctggggta
     7141 aacaggacct cgacgagaac ggcaagaaac ttccctacct ggagctgtat gcgatcaaga
     7201 cgctccccgg gtcggacaag gcaagactgc agggaaccat cgtcgacgat gccagccagg
     7261 atttcgatcc catcacgagt gatgtcgtcg tcgaaatgag catgaacaaa caggcagcga
     7321 agatatgggg cgatatgacg accaagaacg tcaacaaacc catcgcgatc gtgctcgatg
     7381 atatcgtcta ttcggctccc accgtaaatg aacccatcac cggcggctcg tcccggatca
     7441 ccatgggccg cggccgtggc agctcggacc agacgatcac ggaggcaaag gacctggcca
     7501 acatcctgaa atccggtcag ctggacgcac ccgccaagat cgtacaggaa caggtcgtcg
     7561 gacccaccct cggacaggaa gccgtacacg gcggcatgat ggccttcgcc atctccttcg
     7621 tagtcatctt cgccctgatg ctgatctact ataacaccgg cggatgggtc gccaactgcg
     7681 cccttatcct gaacctgttg ttcacgatcg gggtcctcag ttcgctggga gccaccctta
     7741 ccgcacccgg tatcgcgggt ctggtactca cca
//
LOCUS       DASYSV010000166         5174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1184853, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000166 DASYSV010000000
VERSION     DASYSV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184853"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2389
                     /locus_tag="VGQ51_05080"
     CDS             <1..2389
                     /locus_tag="VGQ51_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035966.1"
                     /translation="SEPNKLHLMLTNYLKAYFRNLWKNRTYTFLNIFGLAVGIACSTL
                     IFLWIEDEFTWDHQFTKKAVLYQIQGNQTYGGTTYTFSATPGPLAPAMKTDIPGIAAV
                     SRLCWPYRGLFTTDKNPIYEEGQYSDPEFLSMFNLDFIEGSPATALGDIHSLIISQGM
                     AKALFGNTDPIGKTLKMDHNEPYTISGVFKDLPANCSFHFNWLGSYQAFYQKNQWLQY
                     WGSNGVMTFVELKPSADPASINKLLTNYVQEKAPGAVMKPFLYPMNQWRLYTSFKNGK
                     QDPSNGRIQYIRIFTLIAWIILIIACINFMNLATASSERRSREVGVRKVLGAGKGKLV
                     GQFIGESIVMSFLAVVLSLGLTALALPAFNDLVEKQLSLHLLNPLHITALLAIGLICG
                     LLAGSYPSFYLSSFNPVYVLKGIKVRAAGGAVFIRKGLVVLQFATSIVFIITTIIIYQ
                     QLQYVQHRRLGFEKEGLLYMNLQGKMQDHFTAIRNDLIHTGYVKDAALSRSPVLNLYS
                     NGGGFDWPGKDPGKEVLITQETTSPEYISTMGMQLAAGRNFHEAAAEDSNSIIINESF
                     AHLITAGSAVGTLISNGQNKYRVIGVVRDFVYNNMYQTAAPLILYCYPAGTNYLSVRL
                     KANADVPKAVAAIGNVVRTDNPGYPFEYKFLDEDFLNQFKTETLTGRLSAVFAALAVF
                     ISCLGLFGLAGYAAERRTKEIGVRKVLGASVLLITSLLTRDFVKLVLLACLLAFPVAW
                     WIMHSWLNNYTYRTNIDWKIFLLAGAGAVAIALLTVSYITIRASLANPIRSLRTE"
     gene            2504..4885
                     /locus_tag="VGQ51_05085"
     CDS             2504..4885
                     /locus_tag="VGQ51_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9035967.1"
                     /translation="MLNRLKPALRQFRKYPAFSLINLGGLSIGIAASFLLFVYAHREL
                     NTDRQFHDADRIYRIATDFYHMGPFAFSQAMMCNAVRASCKDVEDATAITAFNNDDVR
                     TSTQDRAFKNNYPYYIDTSFFKVFSYTADAGRIPPGGLGPNEAILSPDYAQRFFGNQD
                     PIGKTIYVGKKMKPYKVVAVLHQGFEKSHLHPRLLLPRGVDSSEYATNWVSCSEFNYV
                     KLRPGATQANLQTWLDRLREKVVYPSTGATISYKEWKDASSTVSFLIQPLTSIYFDNN
                     VKFEIGESGNLTQVHLLRTVAWLLIFLAVINYVNLVTARSSVRSKEMGLKKTFGAGRP
                     ALIAQIISESTAFSLLAMIIACSFIQLILWTYHRTTGADLTGPIPFFSANFFYLFLFT
                     VAVGILAGIYPAFYLTDRRNRLTIRSSGAGKDRPAIRNILVTFQFAIATGLVFVSFTV
                     FSQLNFMKTKDKGFNSDGLVVIDNVGDLKDHAEAFRQLVQQQAAVVSASFCGRTPAGN
                     SIIMGTYLNPATNKNMSIQQFPGDDQYIATLGMRLVGGRNFEKNLISDTNSLILNESA
                     VAALGLFHPIGTLINGSQRVIGVVKDFNYATLREKIAPAVITFQRNDNTGADLIIRLR
                     SGNTAHFIDWLRATAKTFLGDTQLHISFMDDQFAHLAEKERLLGNAIAFFTILAIILA
                     ILGLIGLTIFTIERRMKEIAIRKVLGADNRTILGLVSGRFIRLAAIASAIAIPASWWF
                     IHRWLEHYAYRVSIGVGTLLLTVSLILGIALTIIGGLTIRALAADPINALRRE"
BASE COUNT         1208 a         1759 c         1091 g         1116 t
ORIGIN      
        1 ctccgaaccg aataaactac acctcatgct caccaactac ctgaaagctt attttcgcaa
       61 tctctggaag aaccggacct acaccttcct caatatcttc ggtctcgctg tcggcatcgc
      121 ctgctccaca cttatcttcc tatggatcga ggacgagttc acctgggacc accaattcac
      181 caaaaaggcc gtcctctacc agatccaggg caaccaaacc tacggcggaa ctacctatac
      241 tttttctgcc accccgggcc cgctcgcgcc ggccatgaag accgacatcc ccggcatcgc
      301 ggccgtttcc cgactctgct ggccataccg gggcctcttc accaccgaca aaaatcctat
      361 ctacgaagaa ggccaatata gcgatcccga attcctctcc atgttcaatc tcgacttcat
      421 cgaaggcagc ccggcgaccg cactcggaga tatccattcg ctcatcatca gccagggtat
      481 ggccaaagcc ctgttcggca acaccgaccc cattggcaaa accctgaaga tggatcacaa
      541 tgaaccatat acgatcagcg gcgtttttaa ggacctgccc gccaactgct cctttcactt
      601 caattggctc ggcagctatc aggcattcta tcaaaagaac caatggcttc aatattgggg
      661 cagcaatggc gtcatgacct tcgtcgagct aaagccctcc gccgaccccg cttcgatcaa
      721 caagctattg accaactacg tccaggaaaa agcgccgggc gccgtcatga aacccttcct
      781 ctatccgatg aaccaatggc gtctctatac ctcattcaag aatggcaagc aggatccttc
      841 caatggcagg atccagtaca tccgcatctt taccctcatc gcctggatca tccttatcat
      901 cgcctgcatt aatttcatga acctcgccac cgccagctcg gagcgacgct cccgcgaagt
      961 aggcgtccga aaggtcctcg gcgcaggcaa aggcaaactg gtcggccagt tcatcggcga
     1021 gtccattgta atgtcctttc tcgccgttgt cctatcgctc ggtctcaccg cgctggccct
     1081 tccggcattc aacgatctcg tggaaaagca gctctccctg cacctgctga atccgctcca
     1141 catcacggcg ctgctggcca tcggtctcat ctgcggcctt cttgccggct cctacccctc
     1201 tttctatctt tcctccttta acccggtcta tgtcctaaaa ggcataaaag tgcgggccgc
     1261 cgggggagcc gtatttatcc gcaaaggact cgtcgtcctt caattcgcta cctccatcgt
     1321 cttcatcatc acgacgatca tcatctacca gcaactgcaa tatgtccagc atcgccgtct
     1381 gggcttcgaa aaagaaggcc tcctgtatat gaacctgcaa gggaaaatgc aggaccattt
     1441 cacggccatc cgcaatgatc tcattcacac cggttacgta aaagacgccg ccctcagcag
     1501 aagccccgtc ctcaatctgt acagcaatgg aggcggtttc gactggcccg gcaaagaccc
     1561 cggcaaggag gtcctcatta cccaggaaac cacctcaccc gaatatatct ccacgatggg
     1621 aatgcagctg gcagcaggcc gcaatttcca tgaggccgcc gcagaggaca gcaacagcat
     1681 tatcatcaat gagagcttcg cccatctcat taccgccggc agcgccgtcg gcaccctcat
     1741 tagcaacggc cagaataaat accgggtcat cggggtcgtc agggactttg tctataacaa
     1801 tatgtaccag accgccgcac ccctcatcct gtactgctac cctgcaggca ccaattacct
     1861 ctcggtacgc ctgaaggcga acgccgatgt tcccaaagcc gtcgcagcca tcggaaatgt
     1921 ggtcaggacc gacaaccccg gctacccctt cgaatacaaa ttcctcgatg aggattttct
     1981 caaccagttc aagacagaga ccctcaccgg tcgcctctcc gccgtcttcg ccgcactggc
     2041 ggtcttcatt tcctgcctcg gtctcttcgg cctcgctggc tacgccgccg aaagaagaac
     2101 aaaagagatc ggtgtccgaa aagtgctcgg cgcctccgtg ctcctgatta ccagcctcct
     2161 cacccgcgat tttgtcaagc tcgtcctgct ggcctgcctg ctggccttcc cagtcgcctg
     2221 gtggatcatg catagctggc ttaacaacta cacctaccgc acaaacatcg actggaaaat
     2281 attcctcctt gccggcgcag gagcggtcgc catcgccctg ctgacagtca gctatatcac
     2341 gatcagggct tcgctggcca atcccatccg gagcctgcga accgaataaa ccctcctacc
     2401 ctcgaaatgc aacccccgac ccgtataacc ctcctaccct caaaatgcaa cccccgaccc
     2461 ggataaaccc ttctaccctc aaaataatgt agctttatat catatgctca accgactaaa
     2521 acctgccctc cggcaattcc gcaaataccc cgccttctcc cttatcaacc tgggcggtct
     2581 gtctatcggc atcgccgcct ccttcctact gttcgtctat gcccaccggg agctgaatac
     2641 ggaccgccag ttccacgacg cggaccgcat ctaccgcatc gccacagact tttaccatat
     2701 ggggcccttc gccttcagcc aggccatgat gtgcaacgca gtccgggctt cctgtaaaga
     2761 cgttgaagac gccaccgcca tcacagcttt caataacgat gacgtccgca cctccacaca
     2821 ggaccgcgcc tttaagaaca actatcccta ctatatcgac acttcctttt tcaaagtctt
     2881 ttcctacacc gccgatgccg ggcgcattcc tcccggcggc ctcggaccca acgaggcgat
     2941 cctctccccc gactatgccc aacgcttctt cggcaaccag gaccccatcg ggaaaacgat
     3001 atacgtcggc aagaaaatga aaccctacaa ggtggtcgcc gtcctccacc agggcttcga
     3061 aaaatcgcac ctccaccccc gcctcctcct cccccgcggc gtcgattcca gcgaatatgc
     3121 caccaactgg gtctcctgct ccgagttcaa ttatgtaaag ctcagacccg gcgctaccca
     3181 agccaatctg caaacctggc tcgatcgtct ccgcgaaaag gtcgtctatc catccacggg
     3241 agccaccatc agctataagg aatggaaaga cgccagcagc accgtgtcct ttctcatcca
     3301 acccctcaca tcgatctatt tcgacaacaa tgtaaaattc gagatcggcg aaagtggcaa
     3361 ccttacccag gttcacctcc tccgcacggt cgcctggctc ctcattttcc tggccgtcat
     3421 caactatgtc aatctcgtga cggcccgctc ctccgtccgc tccaaagaga tgggactcaa
     3481 aaagaccttc ggcgcgggtc gccccgccct catcgcgcag atcataagcg aatccaccgc
     3541 tttcagtctc ctggccatga tcatcgcctg cagctttatc cagctcatcc tctggaccta
     3601 tcaccgaacc accggcgccg atctcaccgg ccccataccc ttcttctcag ccaacttttt
     3661 ctatcttttc ctgttcaccg ttgccgtcgg catccttgcc ggcatctacc cggccttcta
     3721 tctcaccgac cggcgcaatc gccttaccat ccgcagcagc ggcgccggta aagacagacc
     3781 ggccatccgg aatatcctcg tcacctttca gttcgccatc gccaccggtc tggtattcgt
     3841 gtcgttcacc gtcttcagcc agctcaattt catgaagact aaagacaaag gcttcaactc
     3901 ggacggactg gtggtcatcg ataatgtcgg cgatctgaaa gaccatgccg aagccttccg
     3961 ccaactggtc cagcaacagg ccgccgtagt cagtgccagc ttctgcggtc gcacccccgc
     4021 cggtaattcc atcattatgg gcacctacct gaacccggct acgaataaga acatgagcat
     4081 acagcagttc cccggcgacg accaatatat cgctacgctc ggtatgcgtc tcgtcggagg
     4141 ccgcaatttt gaaaagaacc tgatatccga taccaattcc ctcatcctca acgaatcggc
     4201 cgtagccgcg ctgggccttt tccatcccat cggtaccctg atcaacggca gccaacgcgt
     4261 tatcggcgtc gtcaaagact tcaattacgc caccctccgg gaaaaaatag cgcccgccgt
     4321 catcaccttt caacgcaatg acaacacagg cgccgacctg atcatacgcc tccgcagcgg
     4381 gaacaccgcc cattttatcg actggctgcg agccaccgcc aaaaccttcc tcggcgatac
     4441 ccagttgcac atctcattca tggacgacca attcgcacac ctggcagaga aagaaagact
     4501 cctcggcaat gccatcgcct tctttaccat actggccatc atacttgcaa tcctgggcct
     4561 catcggtctc accatcttca cgattgagcg acggatgaag gagatcgcca tccgcaaggt
     4621 cctgggcgcc gataaccgca ccattctcgg cctggtttcc ggcagattta tccgcctggc
     4681 cgccatagcc tccgccattg cgatccccgc cagttggtgg ttcatccacc gatggctcga
     4741 acattacgct taccgcgtgt ccatcggcgt gggaaccttg ctattgaccg tatcactgat
     4801 cctgggcatc gcccttacca tcatcggagg cctcaccata cgagccctgg ccgccgaccc
     4861 cataaatgca ttgcgtcggg agtgaaccgc ccggcggctc atggatctcc gtgcagaatg
     4921 attcaaaact gtatcgaaat cggacacttt ttgtatcgaa atcgcacccc gaacgaaaga
     4981 aataccacat aaaaccatgt ttatcatata tttatattct ggcatgtctt ttcaccatat
     5041 cgaaacactg taatctctaa tctatcaata tgatcgagct caaacggatc tctaaatggg
     5101 taaatgtagg cggaagaccc aatttcatct taaaggacat caatctcgaa atcaaagaag
     5161 gggagttcat ctcg
//
LOCUS       DASYSV010000167         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2462423, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000167 DASYSV010000000
VERSION     DASYSV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462423"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..977)
                     /locus_tag="VGQ51_05090"
     CDS             complement(333..977)
                     /locus_tag="VGQ51_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035968.1"
                     /translation="MYPPAIQEDYSIGSVLLMPVMVNIFLIDIFYHIPTGALLNCSLF
                     MLGLIFLLYLRRKELIALFLGAGTQLPDVGKGWMRWSLRLLVIGGAIAIECVVGNNTH
                     SVLEGKWHVDTLRRNGHVFEPDEWLRNSGCWTTAYMEQGGWIVLCPNPYIYDVERSYA
                     GRYEYDAVARRLRGLFDNGKDTLVATVGHYDGSHMEWQGMLGKDTLYMRLSRAK"
     gene            complement(926..1393)
                     /locus_tag="VGQ51_05095"
     CDS             complement(926..1393)
                     /locus_tag="VGQ51_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035969.1"
                     /translation="MSLLALVAVFDVVFTTMGLLGQQNILPKLWRLVGRTFVLQLLFA
                     VVLAVWIAFRWQKAEKHGHVDSLKWYARGQGILRYWLAFGISLYGFAKILKLQFAHGQ
                     MWNDIPVGRLPGLQLTWNYFGHSYAFAVIIALCQIGGCILLLFRRTTLLGRSC"
     gene            complement(1489..1965)
                     /locus_tag="VGQ51_05100"
     CDS             complement(1489..1965)
                     /locus_tag="VGQ51_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEV9035970.1"
                     /translation="MTIQFGYNKRQVLSGLRGHFFGRPEIRILVIVINIFALVSAILF
                     ALKKIQALPFLIFSLLWFCLWITIQRLLPLSIYKRSATFQDTFTLTIEDKGLLLQTSK
                     GQQLWQWDRFSGFKETLYFFHVYFNSRAFFMIPKDSFKDITEIQAARKMLKEKIKK"
     gene            complement(2597..>3936)
                     /locus_tag="VGQ51_05105"
     CDS             complement(2597..>3936)
                     /locus_tag="VGQ51_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035971.1"
                     /translation="DDEIEAANNYVCGTMTVEGAPFLKDEHLAVFDCANKCGKKGQRY
                     IHAHGHIRMMGAAQPFISGAISKTINLPNEATVEEIADAYMLSWKLALKACALYRDGS
                     KLSQPLSTRSDKKKKETEPSSAAGEAAAPVAPATESQIVDLSKLTVKELLEEVHKRVQ
                     ASPDTNLKRALATIVEHRTLPAKRRGFTQKAKINGQTIFLRTGEYADGTVGEIFIDMA
                     KEGATMRSMLNCFAIAISIGLQYGVPLEEFVEKFVFTRFEPSGMVDHPNIKSTTSIVD
                     FIFRSLAYEYLHRTDLVHVLDRPTVGNTGKDDWDLPEEASDDFDSEGRGAAEGRGAEG
                     RVAAEGRAKTPELSDVRIVAKAGGGNSSGGASSGGASSGGASSGGAQAQGSAKAARSA
                     APSARAENGLEAMNAAARSMQSDAPACNTCGHITIRSGTCYKCLNCGNSMGCS"
BASE COUNT          984 a         1174 c          902 g          876 t
ORIGIN      
        1 ttgaaaagct gcgggcgcag attagcaaag aaacgtctct atcgtaaaga cactttaaat
       61 cttgaaaagg ctacctacta agtagataaa attattctgt acgttgctgc aaaaaaattg
      121 cagctttgtg acgaagaaga aagaaaatta tgtatccaac tatgaagaac ctgcgccgtt
      181 gcagctcccg gagacgccct tgcggaccat ctttgaagag tcgaaataca agccgcggta
      241 gccccgcccg ccgcgtttgc gcatgtcgcg tcatcccgcc tgcaacgtcg ccgcatgccg
      301 ccatgccacg caaccccagc cgccgtcatt catcacttcg cccgcgacaa ccgcatataa
      361 agggtatcct ttccaagcat accctgccat tccatatggc tgccgtcgta atggccgacc
      421 gttgcaacca gcgtgtcctt tccgttatcg aacaggcctc gcaaacggcg cgcaacagcg
      481 tcatattcat acctgccggc ataacttcgt tctacgtcgt aaatataggg attgggacaa
      541 agcacgatcc agcccccttg ttccatgtac gccgtcgtcc agcatccgga attcctgagc
      601 cattcatccg gctcaaacac atgcccgtta cgtcgcaagg tgtcgacatg ccatttacct
      661 tccagtacgg aatgcgtgtt attgccgacc acgcactcaa tagcaatggc gccgccgatc
      721 actaacagcc gcaacgacca cctcatccaa ccctttccca cgtcagggag ttgagttccg
      781 gctcccagga acagggctat caattccttt ctgcgcaaat acaggagaaa gatcagaccg
      841 agcataaaca agctacagtt cagaagcgcg ccggtgggaa tatgatagaa tatatcgatc
      901 aggaaaatat tgaccatcac cggcatcagc aggaccgacc caatagagta gtcctcctga
      961 atagcaggag gatacatccg ccgatctggc acaaagcgat gatcactgcg aaagcatacg
     1021 aatggccaaa ataattccag gtgagctgga ggccgggaag cctgcctacc ggaatatcat
     1081 tccacatctg tccatgggcg aactgcaatt tcaggatctt agcgaacccg tagagcgaaa
     1141 ttccgaatgc cagccagtag cggagaattc cctggccacg ggcataccat ttcagcgaat
     1201 cgacgtgtcc atgtttttcc gccttttgcc accggaatgc gatccatacc gccaggacaa
     1261 cggcgaacaa taattgcaat acaaatgtgc gcccgaccaa acgccacagc ttcggaagga
     1321 tattctgctg acccaacaat cccattgtcg taaaaacgac atcgaacacc gctactaaag
     1381 cgagcaaaga cacgccaaac tttggaaacc atggggtgca tgctggcggt gagagtatcc
     1441 tatcgggcga catagtgagg ttattacctt aaaggtacca ttcgcgcctt atttctttat
     1501 tttctccttc agcatcttcc gcgcagcctg tatctccgta atatccttga acgaatcctt
     1561 cgggatcata aagaacgcgc gcgagttgaa ataaacgtga aaaaaataaa gcgtctcctt
     1621 aaatccgctg aacctatccc actgccatag ctgctgcccc ttgctcgttt gcaacagcag
     1681 ccctttgtct tcgatagtca atgtgaaagt atcctgaaat gtagcggatc gtttgtaaat
     1741 gctcaatggg agcaaccgct ggatggtgat ccagagacaa aaccagagaa gggaaaagat
     1801 gagaaaaggc agcgcttgta tcttcttcag ggcaaacagg atggccgata ccagggcgaa
     1861 aatattgatc acaattacca ggatcctgat ctccggtctt ccgaaaaagt gacctcttaa
     1921 gccactcaat acctgccgct tgttgtaccc gaattgtata gtcattccgc aaagtaccaa
     1981 aaaaatgtgc cgggaatgcg ccgcctgact aaaatagcgc attcccggca aaccaataat
     2041 gtctgtcctt cagacgagcc gcgtcccgcg actccaatgc ctgcaccggc cgcggagacc
     2101 ggtacaggaa aaaaaatgcc ctgcgttcgt caatgacggc gcagggcaaa aaaactattt
     2161 tttttatcgc atactcctac aatggctgta gtgattgctg gcggcctggt caggcgttac
     2221 gctacgatca caggaagtaa ggaggctgca agaaaataca atcagtacga atgcggcgat
     2281 gaggcgtttc atggttttca ttttgggttt atggattctt aacgtttaag aacgatacaa
     2341 tattttgcaa gatacggcgg acaaggggtg atttataacc gtttttgcaa aaggggagca
     2401 ggcataggac aaacccgggg ccttataaaa attcccccat gaaaatgggg gaatggaatt
     2461 tatggttaaa accgtcctaa ataattgatt gtcaatgcaa ttcatcccgt ctccgtcctc
     2521 gttaacccgc agcgacccag tcccgatggc cgccgcgccc aagaccatcg ccctggcctc
     2581 gccgaccccg ccatcctcaa ctacacccca tgctattccc gcaattcaga catttatagc
     2641 acgtgccact ccggatcgtg atatgcccgc acgtattaca agccggcgca tcactctgca
     2701 tgctccttgc cgccgcattc atcgcctcca acccattctc agccctcgcg gacggagccg
     2761 cagaccgcgc agccttcgcc gaaccctgag cctgagcccc tcccgatgaa gccccacccg
     2821 atgaagcccc acccgatgaa gcacctccgg agctattccc cccgcctgcc ttcgccacga
     2881 tccgaacatc gctcaactcc ggcgtcttcg cgcgcccttc agccgcaacc cgtccctccg
     2941 caccgcgccc ttcggctgcg ccacgaccct cgctatcaaa gtcatccgat gcttcctccg
     3001 gcagatccca atcgtccttc ccggtatttc cgaccgtcgg ccgatccaat acatgcacca
     3061 ggtccgtacg atgcaggtat tcataggcga gactccggaa tatgaagtcg acgatcgagg
     3121 tcgtactctt gatattcgga tggtcgacca tgccgctcgg ttcaaatctg gtgaagacaa
     3181 acttctccac gaactcctcc aacggcaccc catattgcaa cccgatcgag atcgctatcg
     3241 caaaacagtt cagcatgctc cgcatcgtcg ccccctcctt cgccatatcg ataaagatct
     3301 ctcccaccgt accgtccgca tattcgcccg tgcgtaaaaa tatcgtctga ccgttgatct
     3361 tcgccttctg cgtgaaacca cggcgcttag ccggcaatgt cctatgctcg acgatcgtgg
     3421 ccaacgcgcg tttcagattc gtgtcggggg aagcctgtac ccgcttgtgc acttcttcca
     3481 gcagctcttt caccgttaat ttactcagat cgacgatctg cgattcagtg gcaggtgcaa
     3541 caggtgccgc cgcctccccg gccgccgaag aaggctcggt ctcttttttc ttcttgtccg
     3601 acctggtgct cagcggctgc gacaatttgg aaccgtcgcg ataaagcgcg caggccttca
     3661 gagccagctt ccagctcagc atataggcgt ctgcgatctc ttccaccgta gcctcgttgg
     3721 gaagattgat cgtcttcgaa atggccccgc tgataaaggg ctgggcggcg cccatcatac
     3781 ggatatgtcc atgcgcatgg atataacgct gacccttctt gccgcattta ttggcacagt
     3841 cgaatactgc cagatgctcg tctttgagga acggtgcccc ttctaccgtc atcgttccgc
     3901 atacatagtt gttggcggct tcgatctcgt catccg
//
LOCUS       DASYSV010000168         4295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_803543, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000168 DASYSV010000000
VERSION     DASYSV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803543"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VGQ51_05110"
     CDS             <1..1288
                     /locus_tag="VGQ51_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEV9035972.1"
                     /translation="QYFPRIPELFSENRRRFAGAMEPGSIALFVSNDEMPLNGDAHHP
                     FWQNSDLFWVSGIWQEESMVVLFPGHPDERYREVLVLAKPNELKEKWDGRRLRAEEAR
                     AISGIVTVVWLENIWGMLRQWIHLADSIYLDSNENDRRGGPLITRDYRFIDEMRREFP
                     LHVYRRSAKTLRGLRAIKTPMEVTVMQEAADITGATFGELLKRIRPGVMEYEIEAEIL
                     RGFVSRRASGPAYASIVASGDRARTLHYIANSEECRDGELVLMDFGARYGGYSADLTR
                     TVPVNGKFSKRQAEVYNACLHLHRYAKSLLRPGVGLADYGRQFGEEAGKVFVRIGLLT
                     EGDLKNQTPENMAYSRYMYHGVSHHLGIDVHDLGPRYEPIGEGMVLTVEPGVYIEEEK
                     MGIRIENDVWVTASGCQDLMGEVPITVEEIEAAMKK"
     gene            1343..2107
                     /locus_tag="VGQ51_05115"
     CDS             1343..2107
                     /locus_tag="VGQ51_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV9035973.1"
                     /translation="MKKIPNLFTLLNLVFGCVAIIYILQNGITALYNPDGSQYISMPE
                     KTTIASFCIGMAAVVDFLDGLVARLFKATSPMGKQLDSLADLVSFGVAPGMILYQLLR
                     LGYSGQADGIDVSLGWLLPALIFPCAAAYRLARFNLDESQTYGFKGVPTPAAGILIAS
                     LPLISWYSQNPDIAALIINKWVLYGIIVVVAWLMTSNLPLMALKFKDNTMRGNWPKLL
                     LVGIGVVSAVVLKWVAVPVVFVFYIIVSLVAKNRTS"
     gene            2104..2349
                     /gene="purS"
                     /locus_tag="VGQ51_05120"
     CDS             2104..2349
                     /gene="purS"
                     /locus_tag="VGQ51_05120"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEV9035974.1"
                     /translation="MIYSVQIKVMPLKELLDPQGKAVMGGLQNLGIRSVSDVRIGKSI
                     VLQIEAASAGEAKKIAEEASRQLLANPVMEYFETEVN"
     gene            2349..3032
                     /gene="rsmI"
                     /locus_tag="VGQ51_05125"
     CDS             2349..3032
                     /gene="rsmI"
                     /locus_tag="VGQ51_05125"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV9035975.1"
                     /translation="MLYIVPSPIGNLQDITLRALEVLKVVDLVLAEDTRNSARLLNHF
                     QIVKPLSPFHQHNEHKVLHHLTDQLLQGKKMALLTDAGTPGVSDPAFLLVRECIRVGV
                     TVECLPGATAFVPALVNSGLPMNRFSFEGFLPPKKGRQTLLKQLAGEARTLVIYESPL
                     RLVKTLEEMIVHFGEDRACCVSRELTKIYEENKRGSLRDVCEYFREKGVKGEIVIVVE
                     GCSEKRNEL"
     gene            3050..3505
                     /locus_tag="VGQ51_05130"
     CDS             3050..3505
                     /locus_tag="VGQ51_05130"
                     /inference="COORDINATES: protein motif:HMM:NF022838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HEV9035976.1"
                     /translation="MKKSLYLLLVFGFLMTAGHVDAQFRSIPAVVTDSFKVKYPTAAN
                     VSWSDKVSNFQASFMVGKDRLVAKFDSKGVWLQSWEKITRDEIPAPVKDGLSKSKYAS
                     AEWVIKSVTRYHNPGNVTQYGILVQKSDIQKKNLLFSSDGQLLKDSNTL"
     gene            3510..>4295
                     /locus_tag="VGQ51_05135"
     CDS             3510..>4295
                     /locus_tag="VGQ51_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HEV9035977.1"
                     /translation="MKKLVLAGVIFLAYHPVASAQPDRWQQHVKYTMDVKMDVQTNRF
                     AGTQTLVYTNNSPDTLFEVFYHLYWNAFQPGSMMDSRSRKLGEIVYSGRRGQQQRDWD
                     QRVRDRILNLKPDEIGYQHVLSLKMNGVSQTYETDETILKVKLTKPILPRSSVTFQMD
                     FEAQVPLQIRRSGRDNPQTGVRYSMSQWYPKMCEYDYEGWHPTPYVAREFYGVWGDYD
                     VTISIDKHYILGGTGYLENAAQVGYGYEAPGTKVVRPAGDRLVW"
BASE COUNT          962 a          846 c         1480 g         1007 t
ORIGIN      
        1 gcaatatttt ccaaggatac cggaattgtt cagtgagaac cggaggcggt ttgcgggggc
       61 gatggagcca gggtcgatcg cgttattcgt cagtaatgat gagatgcctt tgaatgggga
      121 cgcgcatcat cctttttggc aaaacagcga tctgttttgg gtatcgggta tctggcagga
      181 agagtcgatg gtggtccttt ttccggggca tcccgacgag cggtaccggg aagtgttggt
      241 attggcgaag ccgaatgaat tgaaagagaa atgggatggc cggcggttgc gtgcggagga
      301 ggcaagggcg atatcgggca tcgttacggt ggtgtggctg gaaaatatct gggggatgtt
      361 acggcagtgg atacacctgg ccgatagtat ctatctcgac tcgaatgaga atgatcgtcg
      421 tggggggccg ctgatcacgc gggattaccg gttcatagat gaaatgcgga gggaattccc
      481 gttgcatgtg taccggcgga gcgcgaagac cctgaggggg ctgcgggcga tcaagacgcc
      541 gatggaggtc acggtgatgc aggaagcggc ggacattacg ggtgcgacgt ttggggagct
      601 tttgaagcgg atacggccgg gcgtgatgga atacgagatc gaggcggaga tcctgcgggg
      661 attcgtatcg aggcgggcgt cgggtccggc ttatgcgagt atcgttgcca gcggggacag
      721 ggccaggacg ctgcattata tcgccaatag cgaggaatgt cgggacgggg agcttgtgct
      781 catggatttt ggcgcgaggt atgggggtta tagtgcggac ctgacgagga cggtgccggt
      841 gaatggaaag ttttcgaagc ggcaggcgga ggtgtataat gcgtgtctgc atttgcatcg
      901 ctatgcgaag agtttgttga gaccgggggt aggtttggcg gattatgggc ggcaattcgg
      961 ggaggaggcg ggtaaggtgt ttgtgcggat cgggttgttg accgagggcg atctgaaaaa
     1021 tcagacgccg gagaatatgg cgtatagccg gtacatgtat catggggtat cgcatcacct
     1081 ggggatcgac gtgcatgacc tgggaccgag gtatgagcct atcggggagg ggatggtgct
     1141 gactgtggag ccgggggtct atatcgagga ggagaagatg gggatacgga tagaaaacga
     1201 tgtatgggtc acggcgagtg ggtgtcagga tctgatgggg gaagtgccga taacggtgga
     1261 ggagatagaa gcggcgatga agaaatgagg ggcggtgcgt cgcgggggct atgaatcgaa
     1321 aataaagcat taattttccg gcatgaagaa aattccgaat ctgttcacgt tgctcaacct
     1381 ggttttcggt tgcgtggcga tcatctatat tttacagaac ggcattaccg cgctgtataa
     1441 tccggatggg tcgcaatata tcagcatgcc ggagaagacg acgatcgcct ctttttgtat
     1501 cgggatggcg gcggtggtag atttcctcga cggattggtg gcgaggttat tcaaagcgac
     1561 gtcgccgatg ggcaagcagc tggattcgct ggcggatctc gtcagttttg gcgtggcgcc
     1621 ggggatgatc ctgtatcagc tgctcaggtt gggttattcg gggcaggcgg atgggatcga
     1681 tgtttcgttg gggtggcttc tgccggcgct gatctttcct tgtgcggcgg cgtacaggct
     1741 ggcacgattt aatctcgatg aatcgcagac ctatgggttc aaaggggtgc ctacacccgc
     1801 ggcgggaata ctgatcgctt ccctgccatt gatctcgtgg tattctcaga atccggatat
     1861 tgccgcgctg atcattaaca aatgggtatt gtatgggatc atcgtggtgg tggcctggct
     1921 gatgacgagc aatctgccgt tgatggcgct gaagttcaag gacaatacga tgagaggcaa
     1981 ttggccgaag ttgttgctgg tggggatcgg ggtggtttcg gctgttgtgt tgaagtgggt
     2041 ggcggtgccg gtggtgttcg ttttttatat tattgtatct ttggttgcta aaaatcggac
     2101 ttcatgatat attcagttca gattaaggta atgcccttga aagaattgct ggatccgcag
     2161 ggcaaggcgg tgatgggagg gttgcagaac ctgggtatcc ggagtgtcag cgatgtacgg
     2221 atcgggaaga gtatcgtgct gcagatagag gccgcgtcgg ccggcgaagc aaaaaagatc
     2281 gcggaagaag ccagccggca gttgctggcc aatccggtga tggaatactt tgagacggag
     2341 gtgaactaat gttgtatatc gttccttctc ccatcggtaa cctgcaggat attacgctca
     2401 gggctttgga ggtgttgaag gtggtggacc tggtcctggc ggaagacacc cggaattctg
     2461 cccggttgtt gaatcatttt cagatcgtga agccgctgtc gccgtttcat caacacaatg
     2521 aacataaggt actgcatcat ttgaccgacc agttgctaca ggggaagaag atggcgctgt
     2581 tgacggatgc gggaacgccg ggagtgtccg atccggcatt cctgctggtg cgggagtgca
     2641 tccgggtcgg ggtgacggta gaatgtctgc cgggtgcgac ggcttttgta ccggcgctgg
     2701 tgaatagcgg gttgccgatg aaccggtttt cgttcgaggg ttttttgccg ccgaaaaagg
     2761 gaaggcagac cttgttgaag cagctggcgg gggaagcgag gacgttggtt atttatgagt
     2821 cgccgttgcg gctggtgaag acgctcgagg agatgatcgt tcattttggg gaggataggg
     2881 catgctgtgt gagccgggaa ttgacgaaga tatatgagga gaataagcgt gggtcgcttc
     2941 gcgacgtttg cgaatatttt cgtgagaagg gggtgaaggg ggagatcgtc atcgtggtgg
     3001 aggggtgttc ggaaaagaga aatgaattat aacctataaa tatgttttta tgaagaaatc
     3061 cctttatctg ctacttgttt ttggattcct gatgacagct ggtcatgtgg atgcgcagtt
     3121 ccggtcgata cccgcggtag tgaccgacag tttcaaagta aaatatccga ccgccgcgaa
     3181 tgtgagctgg tcggacaagg tctcgaattt ccaggccagc ttcatggttg gcaaggacag
     3241 gcttgtcgcc aaatttgaca gcaagggcgt ctggctgcaa tcctgggaga agatcaccag
     3301 ggatgagatc cctgccccgg tgaaggatgg tctttcgaag agcaaatatg ccagtgcgga
     3361 gtgggtgatc aaatcggtga cgaggtatca caatcccggg aatgtcacgc agtatggcat
     3421 cctggtgcag aagagcgata tacagaaaaa gaatttgcta ttcagcagcg acgggcagct
     3481 gttgaaggat agtaatacat tgtaataaga tgaagaaatt agttttagcc ggggtgatct
     3541 ttttggcgta tcaccccgtg gcatcggcgc aacccgatcg ctggcagcag cacgtgaagt
     3601 atacgatgga tgtaaagatg gacgtgcaga cgaaccggtt tgcgggtact cagacgctcg
     3661 tctataccaa caattcccct gacaccctgt tcgaggtctt ttaccattta tactggaatg
     3721 cttttcaacc ggggagcatg atggacagcc ggagccgtaa gctgggcgag atcgtgtatt
     3781 cggggcgccg cgggcagcag cagcgggact gggatcagcg ggtgcgcgac cggatactga
     3841 atctgaaacc ggatgagatc ggctatcaac acgtgctttc gctgaagatg aacggcgtgt
     3901 cgcagaccta tgagacggat gagaccatcc tcaaggtgaa gctgaccaaa ccgatattgc
     3961 cgcgaagctc ggtgaccttc cagatggatt tcgaggcgca ggtgccgttg cagatccgcc
     4021 gcagcggaag agataatccg cagaccggag tgcggtattc catgagccag tggtatccga
     4081 aaatgtgtga atacgattat gaaggatggc atcccacgcc atatgtggcc cgggaatttt
     4141 atggcgtgtg gggggattat gatgtgacga tctcgatcga caaacattat atcctgggag
     4201 ggacgggtta tctggagaat gcggcgcagg tcggatatgg atatgaagct ccggggacga
     4261 aggtcgtacg tccggcgggg gacaggctgg tgtgg
//
LOCUS       DASYSV010000169         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1954138, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000169 DASYSV010000000
VERSION     DASYSV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954138"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /gene="gltX"
                     /locus_tag="VGQ51_05140"
     CDS             complement(<1..1129)
                     /gene="gltX"
                     /locus_tag="VGQ51_05140"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV9035978.1"
                     /translation="MSKRVRVRFAPSPTGGLHLGGVRTVLYNYLFARHHHGDFILRIE
                     DTDQGRYVAGAEEYIMDSLKWAGLEPDESPARPGSYGPYRQSERKPLYAQYAQRLIAA
                     GHAYYAFDTPEELEAMREKHKTADNPSPQYNHDLRKKMRNSLTLDPKETDRLLQEGIP
                     YVVRILLEPDHEVSFTDMVRGHVTFNTSGLDDKVLLKADGMPTYHLAVVVDDFLMEIS
                     HAFRGEEWLPSAPVHILLWRWLFGEDKMPAWAHLPLILKPDGNGKLSKRDGDRLGFPV
                     FAMNWTDPRTGEPATGFRETGFLPEAFVNLLAMLGWTDGTPQEVFTLDELIQRFSMDR
                     VHKGGAKFDFEKAKWFNHEWIRRLPASAYSDQVLELFHAKGI"
     gene            1309..2232
                     /locus_tag="VGQ51_05145"
     CDS             1309..2232
                     /locus_tag="VGQ51_05145"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEV9035979.1"
                     /translation="MLLTLFTASAQITYETVFVDYDSAWTFKNLKIIPIRKKAGGGLP
                     GYGGDLVPLGEALKKGLITVTERGTTSFENVHWLRFNTHTKKSVYISGGEIVSGGRQD
                     RIIVRDTILRPSEKDQYVPVMCVEEGRWSEKEKKFGYGNFANNHLRKVVDSTPNQVLI
                     WGEIDRQLQAGAFKNKSFAYLSRYQDKKYMLKHDEYFRYFMDKFAKTDSNVVGFVALT
                     GDKIIGTDIFAGTDLFYPQLDPLLHGYIDEAINSGAPVMLKDPPVKRYMDPLLKDEAS
                     QEDFVRDKGKIFKDGGMVIHVNTYTKDEPVK"
     gene            2294..2920
                     /locus_tag="VGQ51_05150"
     CDS             2294..2920
                     /locus_tag="VGQ51_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV9035980.1"
                     /translation="MFYLVRTPWLMRKLYPGLVWRVPAVGGKVVYLSFDDGPHPVATP
                     FVLDELKKYDARATFFCIGKNVQKYPHVYRRVLMEGHRVGNHTQDHLDGWKTNDRRYL
                     ENVRDAARLIDSDLFRPPYGKIGLFQSSLLRGAPFRYKIVMWEVLSADFDRRVSPQRC
                     GRNVIRHARPGSIIVFHDSEKAFERMREALPAVLEHFSGLGYRFEAIL"
BASE COUNT          678 a          702 c         1028 g          763 t
ORIGIN      
        1 cgatgccttt tgcatggaag agctcgagga cctgatcgct ataggcagag gcgggcaatc
       61 gacggatcca ttcgtggttg aaccatttgg ctttttcaaa atcgaatttg gcgccgccct
      121 tgtggacgcg gtccatagaa aagcgttgga tgagctcgtc aagggtgaag acttcttgtg
      181 gggtaccgtc tgtccatccc agcatggcca gcaggttgac gaatgcctca gggaggaagc
      241 ccgtttcgcg gaagccggtg gctggttcgc cggttcgcgg gtcggtccaa ttcattgcaa
      301 aaacggggaa acccagtcgg tcgccgtcac gtttacttag tttgccattg ccgtcgggtt
      361 tgaggatcag cggaaggtgc gcccacgcgg gcattttgtc ctcgccgaaa agccagcgcc
      421 acagcagtat gtgaacgggt gcgctgggca gccattcttc accacggaac gcatgtgaaa
      481 tctccataag gaaatcgtct acgacgacgg ccaagtggta ggtgggcatt ccgtcggctt
      541 tgagaagcac cttgtcatcg aggccggagg tgttgaaggt gacgtgaccg cggaccatat
      601 cggtgaagct gacttcatgg tcgggctcga gcaagatgcg aacgacataa ggaatacctt
      661 cctgtaaaag ccggtcggtc tctttcgggt cgagcgtaag cgaattgcgc atttttttgc
      721 gcaggtcgtg attgtattgg ggggacgggt tgtcggcagt cttatgtttt tcgcgcatgg
      781 cttcgagttc ttccggtgtg tcgaaggcgt agtaggcgtg accggcggcg atgaggcgct
      841 gggcatattg cgcgtagagc ggcttgcgct cggattggcg gtaagggccg taggagccgg
      901 gacgggcggg gctttcatcg ggttcgagac cggcccattt cagggagtcc atgatatatt
      961 cttcggctcc ggccacatag cggccctggt cggtgtcttc gatgcggagg ataaaatctc
     1021 cgtgatggtg gcgggcgaag agatagttat ataaaacggt acggacgccg ccaaggtgga
     1081 ggccgcctgt agggctgggg gcaaagcgga cccggactct tttgctcatg cgggcaaagg
     1141 tacggaaacg actggcaaac cgactggaag gaggtttgcg ccgctatgcg gcgcagacat
     1201 tagccctgtg gagaccggtg gcgggggatc ggcgggcggg ggatgcgcga tacaataaat
     1261 tttcggtttc gacgttggga cctcgtgaaa ttgtcgttga ccttctgctt gctgctcact
     1321 ctgtttacgg cgtcagccca gatcacctat gagacggtat tcgtcgatta cgacagcgca
     1381 tggactttca agaatctgaa gatcattccc atccggaaga aagcgggcgg ggggttgccg
     1441 gggtatggcg gcgatctcgt tccgttggga gaggcgttga aaaaggggtt gataacggtg
     1501 acggagcgcg gaacgacttc tttcgagaat gttcactggc tgcgtttcaa cacgcacaca
     1561 aaaaaatccg tctatatttc ggggggagag atcgtaagcg gcggcagaca ggatcggata
     1621 atcgttcgcg ataccatcct gaggccttcg gaaaaggatc agtatgtgcc tgtgatgtgt
     1681 gtggaggagg ggcgatggag cgagaaggaa aaaaaattcg gatatgggaa ttttgccaat
     1741 aaccatctcc ggaaggtggt ggattctaca ccgaaccagg tgctcatctg gggtgagatc
     1801 gaccggcagc tgcaggcagg cgcgttcaag aataagagct tcgcttatct gtcgcgttat
     1861 caggacaaga aatacatgct gaagcatgat gagtatttcc ggtatttcat ggacaaattt
     1921 gcaaagacgg acagcaatgt ggtcgggttt gtcgcattga cgggtgacaa gatcatcggg
     1981 accgatatct tcgcgggcac cgatcttttc tatccgcaac tggatccgct gctgcatggg
     2041 tacatcgatg aggcgatcaa ttccggggcg cccgtcatgt tgaaggaccc gccagtcaaa
     2101 cgctatatgg acccattgtt gaaagatgag gcgagccagg aggattttgt gcgtgacaag
     2161 gggaagatat tcaaagacgg ggggatggtc atccatgtaa atacgtatac gaaggatgag
     2221 cctgtgaaat aggggggagt gccgtgatgc ccgtgacgag tatggcgcgg gggaaattcg
     2281 ctatcttgcc ctcatgttct accttgtcag gacgccgtgg ttaatgagga agctataccc
     2341 tggtctcgtc tggagggttc cggcggtggg agggaaagtg gtatacctta gttttgacga
     2401 cgggccgcat cccgtggcga ctccgttcgt tctcgacgag ttgaagaaat acgatgcccg
     2461 cgctaccttt ttttgcattg ggaagaacgt acaaaaatat ccgcacgtct atcggagagt
     2521 gctgatggag ggacatcgtg tgggcaatca cacgcaggat catctcgacg gatggaagac
     2581 gaatgatcgc aggtatctcg agaatgtccg tgatgccgcg aggctgatcg actccgatct
     2641 ttttcgtcct ccctatggta agatcggttt attccaatcc tctttattgc gcggtgcgcc
     2701 gttccgttat aagatagtga tgtgggaagt gttgagtgcg gacttcgaca ggcgggtctc
     2761 tcctcagcgt tgcggacgga atgtgatccg gcatgcgcga ccgggttcga tcatcgtgtt
     2821 ccatgatagt gagaaggcgt ttgaaagaat gcgggaggca ttgcctgccg tattggagca
     2881 tttttcgggg ttgggttacc ggttcgaagc gatcctttga gtaaataaaa ttgggcctgg
     2941 gtagacaccc tggcccgttt ttaatccgta ccctatgaaa actggtttaa aggtaaatct
     3001 tttttttatt tgtgcaaagt aatttagtca gtcggactgc cggttttgct ggttttaggc
     3061 tttttcgagt gtccgggctg tgcggctggg agcctttttt tcccagggct gcgattcgcg
     3121 ctgcgatgga gtttcagcgt ttggtggggg aggtggggga ggcggggtgc c
//
LOCUS       DASYSV010000170         4855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2338019, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000170 DASYSV010000000
VERSION     DASYSV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338019"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VGQ51_05155"
     CDS             complement(<1..293)
                     /locus_tag="VGQ51_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035981.1"
                     /translation="MHLPLLLIATDHASKSFHQNDILMGKTIVITSGLLAIATWFGAE
                     YLFLLVHASPLVDFALYACFPFFVVVFLVSLFIVRVRHPGAGIATRRAFFVLR"
     gene            383..793
                     /locus_tag="VGQ51_05160"
     CDS             383..793
                     /locus_tag="VGQ51_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV9035982.1"
                     /translation="MKNNPIIASPLAGNMLGQEQGDFVIAEWQDPGGSPGPPRFIAPL
                     HVHYKDDEAWYVLEGCLCVRSGDSIIEARPGCAVFVAKGTVHTYWNPLPQKTRYLLIM
                     TPKIHRLIGDIHAMKDRTTEKMKELWMKYDSAMI"
     gene            complement(799..1233)
                     /locus_tag="VGQ51_05165"
     CDS             complement(799..1233)
                     /locus_tag="VGQ51_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035983.1"
                     /translation="MPVFNDLSLPEREQLEKFPVYLSLLAASKDCRLDGREMHTAVKL
                     THIKTFSCDPVLADFYAEVDKNFETTVREISNQLPADRDAREITIRRELNKLEALLHK
                     LDKRYAAIMHDSLRSYKDHVSRAHRNVLEYFIFPLPISGITD"
     gene            complement(1431..1616)
                     /locus_tag="VGQ51_05170"
     CDS             complement(1431..1616)
                     /locus_tag="VGQ51_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035984.1"
                     /translation="MKTLISLLTLVAIVFAYILSASLYVQGELISAYFLVISSIGAFA
                     FWVESHDLLRVRSHRAA"
     gene            1989..2549
                     /locus_tag="VGQ51_05175"
     CDS             1989..2549
                     /locus_tag="VGQ51_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEV9035985.1"
                     /translation="MPSAVKTFSTWGLVFIAGIIFFSVGILAFFDPLSSYVKLAKFAG
                     VGLLLNGGLLLTVIIVRPMRVRERNWLQCESILHLFFGILFLLNPLLSFIALPFFIGC
                     WLFLAGVIKMLAAISLRTRRRGWTFVLFIGLVFAVFGFLLLFSPFARANSITLLIGSF
                     GVVMGCLYIIDALRFRKMEDTLDMML"
     gene            2618..>4855
                     /locus_tag="VGQ51_05180"
     CDS             2618..>4855
                     /locus_tag="VGQ51_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEV9035986.1"
                     /translation="MRMLLRHLLLAVGLLAFSQAGAQDTTKRPAPQPAATKKDSVRPE
                     RRRSAIYRESFKRVRRKFDSTLFTSNNNPSTSDYAENLEKIYVMLSRVPVVTESFSRL
                     PDIDDQLDQEDSALNILRNRLSQNDRSLNIRNLQMYNTLLDALNANVKSYTRILEHYD
                     TTLDAIRKEIAGLRKDTLMLRIFRDTALKDTFRLQLQQLKDKWRQVDSLVSLNDQLIN
                     SLKSEASAHTITISDLLYRVDHDLEDVGSRAFTKEHRYLWERRTGNRSRYSASDVARA
                     VSDEQQLARFYFANNRNKRFWLLLTGLLFFGWVWFNYRVLRRRSRLAVADNLGIEYIR
                     PYPIAGAMIFMGSLAPLFDLHAPAIYIESIEFLLMIVLTFVLKRRLTRVLFTGWSVFL
                     ILFLLMPLIRLLGMPIWIQRWVEFFVDGISLVFGLLYLFGKGRMTGLPRLIYAAAGLY
                     VLFNFLAVVCNLFGRVTLSQIFGATAVYSFAQTVSLAVFVKLALESFLLQIAASRIRK
                     GYPETFELAAVTKSIRRFTLLLSVILWLIVFTTNLNLFDAFNDWLTDFFGSSREIGDF
                     SFTLGGLVLFLGIIWMAHFLQRFIAYFFGDTGDDAAMDDRGQRSRLLITRLVLLIGGF
                     LLAVAASGLSVDRITVILGALGVGVGLGLQNIVNNFVSGIILIFDRPLRIGDTVEIKD
                     KRGRVKEIGIRSSTLLTEEGAEVIIPNGDVLSNQIVNWTLSNNHVRIMLSFTIEKPPK
                     AEAVEP"
BASE COUNT         1164 a         1251 c         1236 g         1204 t
ORIGIN      
        1 atccgcaaga caaaaaaagc tctcctcgtc gcaatgcctg cacccgggtg gcgtacccgt
       61 acaataaaca gtgaaaccag gaacacaacg acaaaaaagg gaaaacaggc gtacaatgca
      121 aaatcgacca acggcgacgc atgcacaagt aggaacagat attcagcccc gaaccaggtg
      181 gctatagcca gcagaccgct ggtgatcacg atcgtcttac ccatgaggat atcgttctgg
      241 tgaaaggatt tgcttgcatg atccgtcgca attaacaaaa gaggtaaatg catatgtcaa
      301 atatacataa gaaatcaatg tatgtgaaaa ataatttgcc acaggaaaga cgcacaatgg
      361 ataaattgcc cctaaaaaga tcatgaaaaa taacccgata atcgcctccc ctcttgcggg
      421 gaacatgctg gggcaggaac agggggattt cgtaattgcg gaatggcagg accctggtgg
      481 gtctcccggt cccccaagat ttatcgcgcc gctgcacgtc cattataaag atgacgaagc
      541 atggtatgtt ctggaaggat gtctgtgtgt acggtccggc gattccatca tcgaagcccg
      601 accggggtgt gcggtctttg tcgctaaagg caccgtgcac acctactgga atccgctgcc
      661 gcaaaagaca aggtacctgt tgatcatgac tccaaaaatc cacaggctga tcggcgatat
      721 ccatgcgatg aaggaccgga cgactgaaaa gatgaaggaa ttatggatga aatacgacag
      781 tgcgatgatt tgacagaatc aatcggttat gccgctgata ggaagaggaa aaataaaata
      841 ttccagcacg ttgcggtgag cgcgcgatac atggtccttg taggaccgca gactgtcatg
      901 catgatcgcg gcatagcgct tgtccagctt gtggaggagg gcttccagct tgttcaattc
      961 ccttctgatg gtgatctcac gtgcatccct gtcagcgggc agctgattgc tgatctcccg
     1021 gacggtcgtt tcaaaattct tatcgacctc ggcataaaaa tccgccagca cggggtcgca
     1081 ggagaatgtc ttgatatggg ttaacttgac ggcggtatgc atttcgcggc cgtccaaccg
     1141 gcagtctttg ctggcggcta gaagggagag ataaaccggg aacttctcca actgttctct
     1201 ctcgggtaga ctcagatcgt taaaaacagg catgcttcgc aatgttgctc atacaaaatt
     1261 acgctatgta atttacgaaa atcgcgccaa caatggccga tcttgcaaca ttttaacaaa
     1321 gagcatgtta gggatatttc cgccgtgtga agagcgcgtg ttttggggaa ttccctgcct
     1381 gtgtggcggc ctgcgcattc accggcaggc acgcccgatc caagaatatc ctatgcggcg
     1441 cggtgcgaac gaacacgcag cagatcgtgg gattctaccc agaaagcgaa ggctccgata
     1501 gaagagatca ccagaaaata agcagatatc aattctccct gaacataaag ggaagcgctg
     1561 aggatgtagg caaatacgat ggctacaagg gtcagaaggc tgatcaaagt tttcatgatg
     1621 tggtacattt aggtaattaa agactatgat taaaaaagga ataagctatc gaaacagctc
     1681 atagtcattg gacacgaaag gccattacgt aggataccag taaaatacga acgtgatcag
     1741 taagatgaaa aaaaaagtga aggtgtttcg tggagattgg gtacgaatct atttgtctct
     1801 tgtgggcttt tcctgagagg agtattgaat cccttgtggt aatacaaaca tataaaccat
     1861 tttcagtaaa tgcaagcttt taggggcttt tttttgatca atttccgaca acgttttcgt
     1921 acatctactg tatcaatgta ttgtgtaaac gcagtgcgca gaacagatat aattgtgtaa
     1981 atttacccat gccatcggcc gtaaaaacct tttctacatg gggtctggta ttcatcgccg
     2041 gcatcatttt tttcagtgtc gggatccttg cctttttcga tccgctgagc agctacgtga
     2101 agctggctaa attcgcaggc gtggggcttc tgctcaatgg cggacttctc ctcaccgtga
     2161 tcatcgttcg gcccatgcgc gtccgcgaac gcaactggct gcagtgcgag agtatcctcc
     2221 acttgttttt cggtatcctg ttcctgctca atccgcttct ctcttttatc gcactgcctt
     2281 tttttatcgg ctgctggctc tttctggccg gagtgatcaa gatgcttgcc gccatttcgc
     2341 taagaacgcg acgcaggggc tggaccttcg tcctttttat cggactcgtc ttcgccgttt
     2401 tcggcttcct gcttttgttc agcccctttg cccgggccaa tagtattacc ctgctgatcg
     2461 gaagtttcgg ggtggtcatg ggatgccttt acatcatcga tgcgctgcgt ttccggaaga
     2521 tggaggatac attggacatg atgctctgat accccgaagc gggtgcggtg atccccgggc
     2581 ggtaccatgc ggggaatgtt tattatcttt actcataatg cgtatgctat tgcgacatct
     2641 cctgctggcg gtgggattgc tggcgtttag ccaggccggc gctcaggata cgacaaaacg
     2701 gcctgctccc cagcctgcgg caacaaaaaa agattcggtc agacccgaac gcaggagatc
     2761 cgctatttac cgcgagtcct tcaaacgcgt gcgccgaaag ttcgattcga ccctcttcac
     2821 cagcaacaac aatccgtcga ccagcgatta cgccgaaaac ctggagaaga tatacgtgat
     2881 gcttagccgc gtaccggtgg ttacggaatc cttctccaga ctgccggata tagacgacca
     2941 gctggatcag gaggattccg cgctcaatat cctgaggaat cgcctttcgc agaacgaccg
     3001 aagtctcaat atccgcaatc tgcagatgta caatacgctg ctggatgccc tcaacgccaa
     3061 tgtaaaaagc tatacccgta tcctggaaca ctatgatacg accctggacg ccatccggaa
     3121 agagattgcc ggtttgcgca aggacactct gatgctacgt atcttccggg ataccgcatt
     3181 gaaggatact ttccgcctcc agctacagca actgaaagac aaatggcggc aggtcgacag
     3241 cctggtatca ctgaacgatc agctcatcaa ttcgctgaaa tcggaagcct ccgctcatac
     3301 gatcaccatc tccgatctgc tgtaccgcgt cgaccatgat ttggaagacg ttggttccag
     3361 ggcctttacc aaagagcatc gatacctgtg ggagcggcgg accggcaaca gaagccgcta
     3421 ttcggccagc gacgtggcga gagcagtctc cgacgagcaa cagcttgccc ggttctattt
     3481 cgctaacaac cggaataaac gtttctggct gctcttaacc ggtctgctgt tttttggatg
     3541 ggtatggttc aattatcggg ttctccgcag aagaagccgg ttggcggtcg ctgacaatct
     3601 cggcatcgaa tatatccgcc cctaccccat cgccggcgcg atgatcttta tgggcagcct
     3661 ggcgccgctt ttcgatctgc acgcgccggc catctacatc gagtccatcg agttcctgct
     3721 gatgatcgtc ctgactttcg ttttgaagag acgattgacc agggtccttt ttaccggatg
     3781 gtctgtcttc ctgatcctat tcctcctgat gccgttgatc cggctgttgg gtatgccgat
     3841 ctggatccag cgatgggttg aattcttcgt cgatggcatt tccctggttt tcggattgct
     3901 ctatctgttc ggcaagggcc gcatgaccgg tcttccccgt ctgatctatg ctgcggcggg
     3961 cctctatgtt ctgttcaact tccttgctgt cgtatgcaat ctgttcggaa gggttacgct
     4021 ttcccagatc tttggggcga cggcggtcta tagcttcgct caaaccgtaa gcctggccgt
     4081 cttcgtcaag ttggcgctgg aatccttttt gttacagatc gcggccagcc gcatacggaa
     4141 aggctacccc gagacattcg aactcgcagc agtcaccaaa tccatccgaa gatttaccct
     4201 gctactgtcg gtcatcctct ggctgatcgt attcactacc aatctcaacc ttttcgatgc
     4261 gttcaacgat tggctaaccg atttcttcgg ttcatcgcgg gagatcggcg atttttcctt
     4321 caccctgggc ggtctggtgc ttttcctggg catcatctgg atggcccatt tcctgcagcg
     4381 cttcatcgcc tatttcttcg gcgatacggg agacgacgcc gccatggacg accggggaca
     4441 gcgctcacga ctgcttatta cccggctggt cctcctgatc ggcggcttcc tgctggccgt
     4501 cgccgcatcc ggtctatccg tcgaccggat aacggtgatc ctcggagccc ttggcgttgg
     4561 cgtagggctg ggccttcaaa atatcgtcaa taattttgtc tccggcatca ttctcatctt
     4621 tgaccgcccc cttcgcatcg gcgatacggt agagattaaa gacaaaagag gccgtgtaaa
     4681 ggagatcggg attcgcagca gtacgctgct caccgaagaa ggtgcggaag tgatcattcc
     4741 caacggggac gtgctctcga accagatcgt caactggacg ctcagtaaca atcatgttcg
     4801 gatcatgctt tcgtttacga tcgaaaagcc ccctaaagcc gaagccgttg aaccc
//
LOCUS       DASYSV010000171        14352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1954484, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000171 DASYSV010000000
VERSION     DASYSV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 14352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14352
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954484"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..2547)
                     /locus_tag="VGQ51_05185"
     CDS             complement(181..2547)
                     /locus_tag="VGQ51_05185"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9035987.1"
                     /translation="MPRIFFTVVGLFLWLTSCAGFDPIPTAKKGIIDLRQENFFDKSF
                     TINGEWGFYWNRLLDPDSLSSADPVYVPFPRLWNDLKIDGHPIPAQGYATYTLTILLP
                     AKRPRLGMEIPDAYCSLKLYVNGVVQAQNGEPATTRESATPFWVTRNIALPPGERDTL
                     VIVLQVANFWHARGGAYKSILIGDKDELFLKKNQDAAYDFMLCGCLFMGGLFFLALYI
                     FARNDKTILYFALFCILYSYRMVGTSHYALHALFSATNWFITIRIEYLTLSLGITLFT
                     AYTRSLYPEDSHRWVMRGMIGFCLLYSAMVLFTPTLVFTRALVVFLAVMFFCIAYAGY
                     VYMRAARKRRSGSAFALASAGVMLFVFLLGNLHYFGFIPDTRIVLFVCYISFFFLQSL
                     ALSHRFAHSFRQATEQAQQGLRAKSEFLSTMSHEIRTPLNAVIGMAHLLLHNKPRHDQ
                     EDDLGVLLFSANNLLSIVNNILDYNKIEEGKINFEQIPMDLPAIAANIVAGLKNTADE
                     KGIGLRIEVDSRLNKLLFGDPTRTGQVINNLVHNAIKFTRAGYVKLSVIVESMSPESA
                     TVTIKVDDTGIGIPKDKHDVIFDRFTQADSSTSRSYGGTGLGLAISKKILELQGVDLK
                     LQSEPGKGSCFYFTQTFRVDGEETLEASKTLSREGQGPLLNNISILLVEDNPLNVLVA
                     QTMLENNGARVEVATNGAEALERLDASRHQLVLMDLHMPVMDGYEATILLRKRGETLP
                     IIALTASTPKEVESDAFAAGLDDVIVKPFSPDELYRVILLYVHSAPNN"
     gene            complement(2622..3485)
                     /locus_tag="VGQ51_05190"
     CDS             complement(2622..3485)
                     /locus_tag="VGQ51_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9035988.1"
                     /translation="MKLKQILLFAAFPIFLFSCVSKKKLEAAQHQIDSLTAANQKLAG
                     DLKNCNDLTATDANQRTALQNQIDALNKQIDFLKQNHDATLKQLQDLSVISNSQAESI
                     KKSLDNIGAKDIYIQNLQSELAHKDSLNMALVTNLKGAIGNMDDKDINIKVDKGVVYI
                     DISDKLLFKSGSWTVTEAAKTVLGKVATVLKAQPDIEFMVEGHTDNKKFVRPNLQDNW
                     DLSVKRATSVVRILQNQYGLDPAHITAAGRGEYAPVAGNDTEEGRAANRRTRIVILPA
                     LDQFFKLLEKK"
     gene            complement(3708..4817)
                     /locus_tag="VGQ51_05195"
     CDS             complement(3708..4817)
                     /locus_tag="VGQ51_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035989.1"
                     /translation="MKKNMYLLGILMGIMATLCAQSPFDSVPPPETQLFELPYFSIGS
                     AWHFDLGKGNYLELELSDNGQLPRFLNLDSLLLVFIDDMKPFRDSLANPVSGKRIDYL
                     VDTAGRKLVRIHETAPRAATFLLGDDQPSLLRTRMDTVYIFLLAPPLSRTSYLPVRYN
                     RLTIVINRYDRLEELVASGMNNKIKEVDPAAGHRYKADFNGAGHLAIDPSVSIKKEGL
                     YERGHSRDFAESWFTIGAQNYRNMFAPTSAFGLDLVFHHHYDIHTVTLSYGGFVLFGT
                     DAQAHRRTYVNDYISLGYSFDRLDRATLKPVGLFTNFSFGWFVHREGDFLPNNSFLFT
                     FCSLKLMNGRFNLEPGAMFDGGKGSPFIRLSYRFL"
     gene            complement(4829..5515)
                     /locus_tag="VGQ51_05200"
     CDS             complement(4829..5515)
                     /locus_tag="VGQ51_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9035990.1"
                     /translation="MSSERKDEFLSWRGRLDSPEGVPGQGLDDHEQTWERLSDRLREK
                     PRRRLSVYGIVAACLLLAVIPASRFFQDRKPANRSEKRATVAPLVVRPDNGRNVYTSA
                     APAARPVPSAAEPGRRETWTLVRSGGRSKPIIRVDMKTVAVTDDSLHMPVASAVPLVS
                     QLPPAGPVPQKKWKVVDLNELNSGWQPPRATAFNFLPGSLHIGPGKPEPFTAGGPAPD
                     KPAIRIRLSP"
     gene            complement(5493..6050)
                     /locus_tag="VGQ51_05205"
     CDS             complement(5493..6050)
                     /locus_tag="VGQ51_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9035991.1"
                     /translation="MNISEQIEDARSGNAAAQKVLFNRLADPMMALCCRYVKNREDAE
                     EILLDAFYKFFTHLQDFRYQGEAALFAWVRQIMVNECLMFLRRHHSFAIVSEAVAEEI
                     AGEEGDGVDDLSAEEIFGLIVRLPVGYRTVFNLHVIEGMEHGEIAELLGIAEGTSKSQ
                     LHKARLLLRKMLHHKGVSYVQRKKR"
     gene            6238..7239
                     /gene="recA"
                     /locus_tag="VGQ51_05210"
     CDS             6238..7239
                     /gene="recA"
                     /locus_tag="VGQ51_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV9035992.1"
                     /translation="MSNAEKLKALKLTMDKIDKDFGKGSVMMMNERSQDPMEVISTGS
                     IGLDMALGVGGLPKGRIVEIYGPESSGKTTVATHIIAEAQKKGGMCAIIDAEHAFDSS
                     YAQKLGVDVDNLLISQPDYGEQALEIADRLILSGALDVVVIDSVAALVPKGELEGEMG
                     DSKMGLQARLMSQALRKLTATISKTNTICIFINQLREKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRMAQIKDGEEAVGNRVKVKVVKNKVAPPFRAAEFDIVFGEGISKMGEIID
                     MGVELGIVQKSGSWFSYGTDKLGQGRDTVKQLMHDNPELATEIENKIREKVKEMQAS"
     gene            complement(7475..8110)
                     /locus_tag="VGQ51_05215"
     CDS             complement(7475..8110)
                     /locus_tag="VGQ51_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9035993.1"
                     /translation="MKRLACLFLMALVCWYPCHAQLDPSLSDSGSDIHSPKAVHPPFA
                     SFTAVVRESNRVWLQWEVDSIIDGEYFIVERSQDGIHYETIGALLSDNGKMHYELTDQ
                     APPNGSDYYRIRYTGHTGETLYSKPMVLKLSGDVDFKFYPNPVDKFFIVRTEHIVDIQ
                     ILDPAGTVWLSKRLQPGLQIVNASALERGVYILRVTDKESNRAISRQLVKN"
     gene            8262..8753
                     /locus_tag="VGQ51_05220"
     CDS             8262..8753
                     /locus_tag="VGQ51_05220"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEV9035994.1"
                     /translation="MHIPVRKMSGSKRIALVAHDNKKKEIIEWAVYNKTALSKHRLYG
                     TGTTGHLLEEALGQSVSKFLSGPLGGDQQIGARIAEGKIDVLIFFWDPMEAQPHDPDI
                     KALLRLAVAWNIPFACDRATADFLLTSPLMFSDYEIIVPDYTTYTRRLEEPGQAAALQ
                     SPQ"
     gene            8762..11020
                     /locus_tag="VGQ51_05225"
     CDS             8762..11020
                     /locus_tag="VGQ51_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEV9035995.1"
                     /translation="MRKLLFTGSLLLASILALPQSKSGLSGFNDADARQELQLETTFD
                     GYLNPANIDSGIRIMSSHPHHVGSPGDRAVADYIYNKFKSWGYDARIETFYVLFPTPK
                     ERLLEMTGPTSFKASLTEPPLKEDATSGQTRDQLPTYNCYSPDGDVTGDLVFVNYGVP
                     EDYDQLERMGISVKGKIVIAKYGRSWRGIKPKVAQEHGAIGCIIYSDPRDDGYFQGDV
                     YPEGAFKNEYGVQRGSVLDMPIYPGDPLTPGIGATKDAKRLDRKDAAVLLKIPVLPIS
                     YHDAEPLLRALGGPVAPEDWRGALPITYHVGPGKTRVHLKLAFNWDIKPINDVIAMMP
                     GTEWPDEWVIRGNHHDGWVNGAADPISGQSALLEEARGLALLHKSGWSPKRTIVFCAW
                     DGEEPGLLGSTEWAEEHADELRQKAVVYINSDGNGRGYLQAGGSHALEPFMNEVARDV
                     MDPQTHISVADRRHDRELVSAAGAGRLKKDLLQKKTFTLSALGSGSDFSAFLQHLGIP
                     SFDLGFGGEDPGGEYHSIYDSYDNYRRFKDPSFVYGVTLAKTAGRTTLRMADAPLLPF
                     DFRALFKTINGYVTDLMTSLDQMRETTALTDQLLKDKAFSYATDTARHLLPPPAHDEV
                     PYLNFSALQNSLEVLDHAINHLADSLAKARLSGDREKAVNEALYRAEQQLLSDNGLPR
                     RPWYRHIIYAPGFYTGYGVKTMPGIREAIEQRNFTEAQQQIEIDAAVLRKFADYLSAI
                     FGSPGKTGVAKQ"
     gene            11091..13583
                     /locus_tag="VGQ51_05230"
     CDS             11091..13583
                     /locus_tag="VGQ51_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEV9035996.1"
                     /translation="MKKFARYTIRIAGTILCLLLLAWLCLVAYIQLNKAALLQKAKTQ
                     LSARISGEVRIGGLDISFFRYWPSVAVRLTTVSVRDSAWQNHHHDLLTAATVDLTCNL
                     WKSLRTFRIQTGSVDISHGQIYLFTDSAGYSNDYLLHVRGRSDPEGPGSSPSYPEITL
                     TDTRVVMEKQDKHKFFDLDVRHLACSIEKQDRSLLLHVRAGNIRIAGFAFNTEKGSFL
                     KDKPLSGNFTLQYNTASKILRFEKARLLIDGIHFLFSGRLFPAVKPDPFLLEVQTDNI
                     GYRQATALLTPDLQQKLDKYNIDKPVAIKARLDAGAADEPTPQIKVELTLSDGGVLTP
                     VGRFTGVSFKGSFINEWIHGRKRDDMNSGIRFVAFTGELQYIPLRSDSITITNLKNPV
                     MSCDLHSQFGLSKLNDLTGSETLWFTGGTGNLNIVYKGPLSENDTAGTVVNGNLDIDS
                     AKLLYLPYNFTLSDGRGRLAFKDQDLEIEKLGIRAGSSNFQVSGKARNLVALLDQNSE
                     NVSMDWNLTSPRLDLEDLVALAGRSTPVTTKQNTVSLFRSTFSRIDRLLKEGAIHVGI
                     EAADLRFRKFSGTHAKADLIFNDRSIRLNRLSVAQGSGAIELKATLSRLAPGESSPLI
                     LESHLEKVDLPRIFASFNNFGQDAIEAQNLKGQLTADARMTGKLTGKAKIVPNSLKGS
                     VGFRITDGELVDFPPMEKIKVGFLKNRDLSAVRFSGLENELDVDSTTVTIHRMEINSS
                     AFTLFAEGTYDLKTGPDISLQIPLRNLSDNKYQSIPPESRGNDGKAGPSIRLRAHRDD
                     EGKLKITWDPFKRALKKEKRASRSKGREAKSS"
     gene            13610..>14352
                     /locus_tag="VGQ51_05235"
     CDS             13610..>14352
                     /locus_tag="VGQ51_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV9035997.1"
                     /translation="MPFKINSPFPPSGDQPEAIRQLTEGIQQGEPYHTLLGVTGSGKT
                     FTIANVIQNVQRPTLVLTHNKTLVAQLYGEFKQFFPENAVGYFVSYYDYYQPEAYMPV
                     SNTYIEKDLSINEELEKLRLQATTQLLSGRRDIIVVASVSCIYGIGNPAEFENGIIRL
                     QQGQTISRQGFLHALVNSLYKRTEIEFARGNFRVKGDTVDINLPYVDYGYRISFFGDE
                     IDSIESFDISSGKRIVAMDNAAIFPANLYL"
BASE COUNT         3535 a         3942 c         3781 g         3094 t
ORIGIN      
        1 tttcgatggg atgcgtcact ggagcctcgg tcggtgattg acaggcggta aagagcgaaa
       61 ggcagatcac ggctagacat ttcatgagat aggttttatc cggatggaaa gtggaaaaac
      121 tatgccaaat gcaaatgagg aagattttcc ccagggtctc gcggcgcccc gcggccgcta
      181 ttaattattg ggcgcgctat gtacgtacag caggatgaca cgataaagtt catcggggct
      241 gaatggcttt acgatgacgt cgtcgagacc tgccgcaaag gcgtcgcttt ccacttcttt
      301 gggggtactg gctgtgagcg cgatgatggg cagggtctct ccccgtttgc gtagcaggat
      361 ggtggcttca taaccatcca ttacgggcat gtgcaggtcc atcaatacga gttgatgcct
      421 ggaggcatcg agcctctcga gcgcctcggc gccgttggtg gccacctcta cacgggcgcc
      481 attgttctct agcatggtct gcgccaccag cacgttcagc ggattgtctt ccaccagcag
      541 gatggaaata ttgttcagca acggtccctg gccctctctc gaaagggtct tgctggcttc
      601 cagtgtctct tcaccgtcga cccggaaagt ctgtgtgaag taaaaacagg agccctttcc
      661 cggttcgctt tgcagcttca gatcgacgcc ttgcaattcc aggatcttct tcgaaatggc
      721 caatcccaga cccgtgccgc catagcttcg tgaggtagaa gaatccgctt gcgtaaaccg
      781 gtcgaatatc acatcgtgtt tatctttcgg aatgccgata cccgtatcat ctaccttaat
      841 ggtgacggtc gcggactcag gtgacatcga ttctacgatg acggagagtt ttacgtatcc
      901 cgccctggtg aatttgatcg cattatggac aagattgttg atcacctggc ccgtccgggt
      961 cggatcgccg aaaagcaatt tgttcagccg gctgtcgacc tcgatacgca agccgatgcc
     1021 tttctcatcg gccgtattct tcaatccggc tacgatattc gcggcgatgg cgggaagatc
     1081 catcgggatc tgctcgaaat tgatcttgcc ttcttcgatc ttgttatagt cgaggatatt
     1141 gttgacgatg gaaagcaggt tgttggctga gaaaagcaaa acgccaaggt cgtcctcctg
     1201 gtcatgtctc ggcttgttat gcaacaacag gtgagccatt ccgatcaccg cgttcagggg
     1261 cgtcctgatc tcgtgcgaca tcgtcgaaag gaattcggat tttgcccgga gaccctgctg
     1321 agcctgttct gtagcctgcc tgaaagaatg agcgaatcga tgtgataatg ccagggactg
     1381 aagaaagaaa aaggagatat agcacacgaa gagcacgatc ctggtatccg gtatgaaacc
     1441 gaaataatgc aggttaccga ggaggaatac aaacagcatc actcctgcgc tggccagcgc
     1501 gaaggcagac ccgctgcgcc gtttgcgtgc ggcgcgcata tatacatagc cggcataggc
     1561 gatgcaaaag aacatgaccg ccagaaagac caccagcgcc cgggtgaaga ccagcgtagg
     1621 tgtgaaaagg accatcgcgg agtacaaaag acagaatcct atcattcccc gcatgaccca
     1681 ccgatgcgaa tcctcggggt aaaggctccg ggtatacgcc gtgaacaggg tgatgcccag
     1741 cgaaagcgtg aggtattcta tacggatcgt gatgaaccag ttggtagcgg aaaaaagagc
     1801 atgcaatgca tagtggctcg tgcctaccat acgatagctg tagaggatgc agaacagggc
     1861 gaaataaagg atggtcttgt cgttgcgtgc gaaaatatac agtgccagga aaaaaagacc
     1921 tcccatgaaa agacagccgc acaacatgaa atcataggcg gcgtcctggt tcttcttgag
     1981 gaacagctca tccttgtcgc cgatgagaat gcttttatag gctcctccgc gggcatgcca
     2041 gaaattggcg acctgcagca caattacgag cgtatcgcgc tcgcccggcg gaagtgcaat
     2101 attccgggtg acccaaaacg gggttgccga ttcgcgcgtc gtcgccggtt cgccattctg
     2161 agcctgaacg acaccgttca catataattt taaggagcaa tatgcgtcgg ggatctccat
     2221 gcccagacgc gggcgtttgg ccggaaggag gatcgtcagt gtataagtcg catagccctg
     2281 ggccggtatc gggtgcccgt cgatcttcag gtcgttccat aaccgcggaa agggaacata
     2341 gaccggatca gccgaggaga gactgtcagg gtccagcagc ctgttccaat aaaaccccca
     2401 ctctccattg attgtaaatg acttatcaaa aaaattctcc tgcctgaggt cgatgatccc
     2461 ttttttcgca gttggaattg gatcgaatcc ggcgcaggag gtcagccaca gaaagagccc
     2521 caccacagta aaaaagattc tgggcatgga tgataacgaa aaagatggcc cgctgagtat
     2581 aacagactcg cgggccattt attattattt ggaaaccggg tttacttctt ttcgagaagt
     2641 ttgaagaact gatccagcgc tggcaggatc acgatccggg tccggcggtt ggctgcacgg
     2701 ccttcttcag tgtcattgcc agcgacggga gcgtattccc cacgacctgc ggcagtgata
     2761 tgggcaggat cgaggccata ttgattctgg aggatgcgga cgaccgaggt ggcgcgttta
     2821 acgctgaggt cccagttgtc ctggaggttc ggacgaacga atttcttgtt gtcggtatga
     2881 ccttcaacca tgaactcgat atcgggctga gccttgagta cggtagcgac cttacccaat
     2941 acggtctttg cggcttcggt taccgtccag ctaccgctct tgaaaagcag cttatcggag
     3001 atgtcgatat agacaacgcc tttgtccact ttgatgttga tgtccttgtc gtccatattg
     3061 ccgatggcgc ctttcaggtt cgtcaccagc gccatgttca gggaatcctt atgtgcgagt
     3121 tcggattgga ggttctggat ataaatatcc tttgccccta tgttatccaa agattttttg
     3181 atgctttccg cctgcgagtt cgaaatgacg gagagatcct gcaattgctt gagggttgcg
     3241 tcgtgattct gtttcaggaa atcgatctgt ttgttgaggg cgtcgatctg gttctgcagc
     3301 gccgtccttt ggttagcgtc cgtagcggtc aggtcgttgc aatttttcag gtcgccggcc
     3361 agtttttgat tggcggcggt aagggagtcg atctggtgtt gggcagcttc taattttttc
     3421 ttactgacgc aggaaaatag aaagataggg aaggcagcaa aaagaagaat ttgtttaagc
     3481 ttcatagtaa tatcattttt ttagaaaata taccttcccg gcgcgtgtta cggggcaacc
     3541 tgagaagaat aatgtgccaa agatagttca acaaaccaga aaagctaatc tgatttggca
     3601 aaaatagccc aatattgtgc caaattcctc gaatgcccat aatttgcaaa cacacgcgca
     3661 atgggacaaa gcgggcgtct atggcccgcc ggaagaagcc gctcgggtta caggaaacgg
     3721 tagcttaaac gtataaaagg agagccttta ccgccgtcga acatggcccc cggttcgaga
     3781 ttgaaccggc cgttcatcag cttgagacta cagaaggtga acaggaagct gttgttcgga
     3841 agaaagtcac cctcgcgatg gacgaaccag ccgaatgaaa aattggtgaa aagacccacc
     3901 ggtttcaacg tggcccgatc gagacggtcg aaagaataac cgagcgagat atagtcgttg
     3961 acatacgttc gccggtgcgc ctgagcgtcg gtgccgaaga gtacgaagcc gccgtaggag
     4021 agagtgaccg tgtggatgtc ataatgatga tgaaaaacga ggtcgaggcc aaaagccgat
     4081 gtcggggcga acatgttgcg gtaattctgc gcgccgatcg tgaaccacga ttcggcaaaa
     4141 tcccgggaat gccccctctc gtacaaacct tcttttttaa tactcaccga tggatcgatc
     4201 gccaggtgac cggccccgtt gaaatccgct ttataccggt gcccggccgc gggatccacc
     4261 tcctttatct tattgttcat accgcttgcc accaattcct cgagccgatc gtaccggttg
     4321 atcacgatgg taagccggtt gtaacgaacc ggcaaataac tggtccggga caaaggcgga
     4381 gcgagcagga agatgtaaac ggtgtccatt cgggtcctga gcagggatgg ctgatcgtct
     4441 cccaacagga aggtagcggc gcgcggcgcg gtctcatgga tacgaacgag ctttctgccg
     4501 gccgtatcga ccaggtaatc tatccgtttg cccgaaacgg ggttggccag agagtcacgg
     4561 aatggcttca tatcgtcgat gaagacgagg agcagcgaat cgaggttcag gaaacggggc
     4621 agctggccat tatcgctcag ctccagctcg agataattgc cttttcccag gtcgaaatgc
     4681 caggcggacc cgatggaaaa gtaaggtagc tcgaagagtt gggtctcggg cggaggaacg
     4741 gagtcgaatg gggactgggc gcaaagcgtt gccattatgc ccatcaggat gccgagcaaa
     4801 tacatgtttt ttttcatact gtatattttt aaggggaaag cctgatcctg atcgccggtt
     4861 tgtcgggggc cggccctccg gcagtgaagg gttcgggctt acccggaccg atatgtagtg
     4921 atccgggaag gaaattgaaa gccgtcgcac gcggaggttg ccagccggaa ttgagctcgt
     4981 tgagatcgac gaccttccat ttcttttgcg gcaccggtcc tgccggcggt aattgcgaca
     5041 ccagggggac agccgacgct accggcatat ggagcgagtc atcggtcacc gccaccgtct
     5101 tcatatctac ccggatgatc ggttttgacc ggccgcccga ccttaccagg gtccaggttt
     5161 cccggcgacc gggttctgct gccgacggaa ccgggcgagc cgccggggct gccgaggtat
     5221 agacatttcg tccattgtcg ggacggacga ccagcggggc gactgtcgct cttttttcgg
     5281 agcggttggc cggctttcga tcctggaaaa aacgcgaggc ggggatcacc gccaacaaca
     5341 gacaggcggc tacgatacca tatacggaca ggcgccgccg cggcttttca cgtaaacgat
     5401 ccgacagacg ctcccatgtc tgttcatgat cgtcgagccc ctgtccggga accccttccg
     5461 gtgagtcgag acgaccgcgc caggacaaaa actcatcttt tctttcgctg gacataactg
     5521 acacctttgt gatgaagcat ttttctcaat aataatctgg ccttgtgcag ctgggatttc
     5581 gacgtgcctt cggcgatccc aagcagctcc gcgatctcgc cgtgctccat gccttcgatg
     5641 acatgcagat tgaagaccgt ccggtaacct accggaagac gcacgatcaa cccgaatatc
     5701 tcttccgccg acagatcgtc cacgccgtcc ccttcctctc ccgcgatctc ctccgcgacg
     5761 gcctccgata cgatcgcaaa ggaatgatgc cgccgaagga acatcaggca ttcgttgacc
     5821 attatctgcc gcacccaggc gaaaagcgcc gcctccccct ggtaccggaa gtcctgtaaa
     5881 tgcgtaaaga acttatagaa cgcatccagc aggatctctt cggcatcctc ccggttcttc
     5941 acataccggc agcacaatgc catcatggga tccgccaggc ggttgaaaag gactttttgc
     6001 gcggcggcat tccctgatcg tgcatcttcg atctgttccg atatattcat atttggcgct
     6061 ccgttgggat aaatgcaaat tacgaatgcc gggttgcccg gcgtccaaaa aaacgcggca
     6121 tgctacgtgt atccaaaaaa gttagtgaaa aaatactaat aatattagtg tgtataaaat
     6181 atttaccata cctttagcca atcaataaat ttggggaaca aacataaaac cacgactatg
     6241 tcaaatgcag aaaaactcaa ggctttgaag ctgacgatgg acaaaataga caaggatttc
     6301 gggaaaggaa gcgtaatgat gatgaatgaa agaagccagg atcccatgga ggtcatttcc
     6361 accggtagta tcgggttgga tatggcgctg ggggtcggag gtctgcccaa aggcagaata
     6421 gtagagatat acggccccga atcatccggt aaaacgaccg ttgccacaca tatcattgcc
     6481 gaagcacaga aaaaaggagg catgtgcgcg atcatcgacg ccgaacatgc tttcgacagc
     6541 agctatgctc agaaactggg tgtcgatgtc gacaatctcc tgatctccca acccgattat
     6601 ggcgaacagg ccctggaaat agccgaccgg ctgatccttt ccggcgcact ggatgtcgtc
     6661 gtgatcgact ctgtcgccgc acttgtccct aagggtgagc tggaaggtga gatgggagat
     6721 agcaaaatgg gcctgcaggc acgcctgatg tcacaggcac tcagaaagct tacggccacc
     6781 atcagcaaga ccaacaccat ctgtatcttc atcaatcagc tgcgggaaaa gatcggcgtg
     6841 atgttcggca accccgagac gaccacgggg ggcaatgcac tcaaattcta tgcttccgtc
     6901 cgtctcgata tccggcgtat ggcacagatc aaggatggcg aagaagcggt gggcaaccgg
     6961 gtgaaagtaa aggtcgtaaa gaacaaagta gcgccgccat tccgtgccgc cgaattcgat
     7021 atcgtattcg gagaaggtat ctccaagatg ggcgagatca tcgatatggg cgtagagctc
     7081 ggcatcgtac agaagagcgg tagctggttc agctatggca ccgataaact cggacagggt
     7141 cgcgataccg taaaacaatt gatgcatgac aatcccgaac tcgcgacaga gatcgagaac
     7201 aagatccggg agaaagtcaa ggaaatgcaa gcctcgtaaa cacgcaattt aaatttggtt
     7261 agtaatacca gggccccgat ttctatcggg gcttttttat ttcgcaagat gggaagaacg
     7321 cggatcggaa ccggctgaaa ataaaaaagg ggtctgaata gaaattcaac cccgttttaa
     7381 aatccaatat cctatgaaaa accaagataa aggtatagga ataaaatcaa atctgcaata
     7441 ccattttatg gtaatatttc tttttaagtc gtttttaatt cttcaccagc tgtctggaaa
     7501 tcgctctatt gctttcttta tcagtaactc ggagaatata gacccctctt tccagcgcag
     7561 aggcgttaac gatttgtaaa ccgggttgca atcgcttgct caaccatacc gtacctgcag
     7621 ggtccaggat ctggatatcg acgatatgct cggtcctaac gatgaaaaac ttgtctaccg
     7681 ggttaggata aaacttgaaa tcgacatccc cggaaagctt gagcaccatt ggttttgaat
     7741 aaagcgtttc gcccgtatgg ccagtatatc gtattctgta ataatccgaa ccgttgggcg
     7801 gcgcctgatc cgtgagctca taatgcattt tcccattatc ggaaagcaac gccccgatgg
     7861 tctcatagtg aatgccgtcc tgactgcgtt cgacgatgaa atattcccca tcgataatgc
     7921 tgtcgacctc ccactgcagc caaaccctat tcgattccct gaccactgcg gtgaaactgg
     7981 caaacggcgg atggactgcc ttaggggaat gtatatccga accggaatcg gacaacgaag
     8041 ggtccaattg agcatggcag ggataccagc aaacaagtgc cataagaaaa agacaagcta
     8101 gtctcttcat tgccgttggg ttagtaagta caacgtaaaa gggtcgatat ctcaacaaat
     8161 tctatgcctg aaggatagaa cgctgcatga cgcgcaaaat tttttacccg attgttataa
     8221 tcttcttaaa acacgactac cttgaggaaa caaatctcta tatgcatatt cccgtgagga
     8281 agatgagtgg ctccaaacgc atagccctcg tggcacacga caataaaaag aaggaaatca
     8341 tcgaatgggc cgtttataac aagaccgccc tttccaaaca ccggttatac ggcaccggca
     8401 ccaccggtca cctgttagaa gaagccctcg ggcagtcggt aagcaagttc ctgagcggtc
     8461 cccttggcgg tgaccagcag atcggcgccc gtatcgctga aggcaagatc gacgtcctca
     8521 tctttttctg ggaccccatg gaagcccagc cccatgaccc cgatatcaag gcgcttctcc
     8581 ggctggcggt agcgtggaat atccccttcg cctgcgaccg ggcaacggcc gacttcctgc
     8641 tgacctcccc gctgatgttc tccgattacg agatcatcgt ccccgattat acgacctata
     8701 cccggcgcct ggaggaacca ggccaggctg ccgcacttca atcacctcaa taaaaatgca
     8761 catgagaaaa ctccttttca cggggagcct gctgctggct tccatcctcg cccttcccca
     8821 gtcgaagtct gggctgtccg gtttcaacga cgccgatgcc agacaggaat tacagctcga
     8881 aaccacgttc gacggctacc tcaatcccgc caatatcgac agcggcatcc gtatcatgtc
     8941 ctcccatccg catcatgtcg gctcgccggg cgacagggcc gtcgccgact acatctacaa
     9001 taaattcaaa agctggggat acgatgcccg tatcgaaacc ttttatgtcc tcttccctac
     9061 ccccaaagaa cggctgctcg agatgacagg cccgacttcc ttcaaggcca gcctcaccga
     9121 accgccattg aaagaagacg cgacttcagg ccaaacccgc gaccaactcc caacctacaa
     9181 ctgctattcg ccggacggcg atgtcaccgg tgacctcgtt ttcgtcaact acggcgtccc
     9241 cgaagattat gaccagctgg aaaggatggg catctccgtg aaaggaaaga tcgtcatcgc
     9301 caaatacggc cgctcctggc gcggcattaa acccaaggta gcccaggaac acggcgccat
     9361 cggctgtatc atctactccg acccccgcga cgatggctat tttcagggag acgtctaccc
     9421 cgaaggcgcc ttcaagaacg aatatggtgt tcaacgcggc tcggtgctcg atatgcccat
     9481 ataccccggc gacccgctga cgcccggcat cggcgccacg aaagatgcca aacggctgga
     9541 tcgtaaagac gccgccgtcc tgctgaagat ccctgttctg cctatcagtt atcacgatgc
     9601 cgaaccactc ctccgcgctc tcggaggtcc tgtcgcgccg gaagactggc gcggagcgct
     9661 tcctataacg tatcatgtgg gaccgggtaa gactcgcgtc catctgaagc tggccttcaa
     9721 ctgggatatc aaacccatca acgacgtaat agcgatgatg ccgggcaccg aatggcctga
     9781 cgaatgggtc atccgcggta atcaccacga tggctgggtc aacggcgccg ccgaccctat
     9841 cagcggtcag tctgccctgc tcgaggaagc ccgcggctta gcccttctgc acaaatccgg
     9901 ctggagtccc aaacgcacca tcgtcttttg tgcctgggac ggtgaagaac cgggtctgct
     9961 cggctctaca gaatgggccg aagaacatgc cgacgaactt cggcagaaag cggtggtcta
    10021 tatcaattcc gacggcaacg gccgtggtta cctgcaagca ggcggctcac atgcgctcga
    10081 acccttcatg aacgaagtgg cccgtgacgt gatggatcca cagacccata tcagcgtcgc
    10141 cgaccgcaga cacgatcgcg agctcgtcag cgcagcgggt gccggccggc tcaaaaaaga
    10201 cctgcttcag aaaaagacct tcaccctctc ggctttgggt tccggttcag acttctcggc
    10261 ctttctccag cacctcggca tacccagctt tgacctgggt ttcggaggag aagaccccgg
    10321 cggcgaatac cattccatct atgattcgta tgacaactac aggcgattta aagaccccag
    10381 ctttgtatat ggggtaaccc ttgccaaaac cgcaggtcgt acgaccctgc gcatggctga
    10441 cgcacctctt ctccccttcg atttccgggc gctgttcaaa accatcaacg gatatgttac
    10501 cgatctgatg acctctctag accagatgcg tgaaaccacg gcgctgaccg accaattgct
    10561 caaggacaag gccttctcct atgccaccga taccgcccgg catctcctgc cgcctcccgc
    10621 acacgacgag gtaccctatc tcaacttttc agcgctgcag aactccctcg aagtcctcga
    10681 ccatgccatc aatcacctgg ccgactcgct ggccaaagcc aggctcagcg gcgatcgcga
    10741 aaaggccgtc aacgaggcgc tctacagagc ggaacaacaa ttgctgtcgg ataatgggtt
    10801 gccccgccgt ccatggtatc gtcatatcat ttatgcacct ggcttctaca ccgggtatgg
    10861 cgtaaaaacg atgcccggga tacgcgaagc gatagaacaa cgcaatttta ccgaggccca
    10921 gcagcagatc gagatcgatg ccgccgtcct gaggaagttc gccgactatc tctcggctat
    10981 tttcgggagt cccggaaaga cgggagtcgc caaacaatag cggcgccgct cccacgcggg
    11041 gtcgccaaac aatagcggct cagttcccgc gcggagcaac ccgcaactga ttgaaaaaat
    11101 tcgcacgata cacgatacgc atagcaggca cgatcttgtg cctgctttta cttgcctggc
    11161 tctgcctggt cgcatacatc cagctcaata aagccgcgct cctccaaaaa gcaaagaccc
    11221 agctaagcgc ccgtatttcc ggggaggtcc ggatcggcgg tctcgatatc tcctttttcc
    11281 gttattggcc atcggtcgcg gttaggctca ccacggtgtc ggtccgcgac agcgcctggc
    11341 aaaaccacca tcacgatctc ctgacagcgg ccaccgtcga tctcacctgc aatctctgga
    11401 aaagcctccg gacattccgg atccagaccg ggagcgtcga tatcagccat gggcaaatct
    11461 atctgtttac ggatagcgcg ggctatagca atgactatct tttgcatgtc agagggcgct
    11521 cggacccgga ggggccggga agctcccctt cctacccgga aatcaccctg accgatacac
    11581 gggtggtcat ggaaaaacag gacaaacaca agttcttcga tctggatgtc cgccatctgg
    11641 cctgctccat cgaaaaacaa gaccggtcac tgctactgca tgtccgcgca ggaaatatcc
    11701 gcatcgccgg cttcgccttc aacacagaaa aaggcagctt cctgaaagac aagcccttgt
    11761 cggggaattt tacccttcag tacaacacgg ccagcaagat cctccggttc gaaaaggcaa
    11821 gactccttat cgacgggatc cacttccttt tttccggccg cctttttccc gccgtgaagc
    11881 ccgacccctt tctcctcgag gtgcagacag acaatatcgg gtaccggcag gcgacggcgc
    11941 tgctgacccc cgacctgcag cagaaactgg acaaatacaa catcgataag ccggtggcca
    12001 tcaaagcccg tctggatgcc ggcgctgccg atgaaccgac accccagata aaggtggagc
    12061 tgacactttc cgatggcggc gttctgacgc cggtgggccg gtttaccggc gtgtccttca
    12121 aaggaagctt catcaacgaa tggatacatg gccggaaacg tgacgatatg aactccggta
    12181 tccggttcgt ggcttttacc ggtgagcttc agtacatccc cctccgctcg gacagtatca
    12241 cgatcaccaa tctgaaaaac cccgtcatgt cctgcgatct gcattcacaa ttcggattga
    12301 gcaagctgaa tgatctcacc ggcagcgaga ccctctggtt taccgggggg acgggtaacc
    12361 tgaatatcgt atataaaggt ccattatcgg agaacgatac cgccggaacg gtcgtcaacg
    12421 gcaatctcga catcgattcg gcaaaactgt tgtatctgcc ctataacttc acgctttcgg
    12481 atgggagggg ccgtctggcc tttaaggatc aggacctgga gatcgaaaag ttggggatcc
    12541 gggcggggag ctccaatttt caggtaagcg gcaaggcccg caacctcgtc gccctgctcg
    12601 accagaattc ggagaatgtg agcatggact ggaacctcac cagcccccgt ctcgatctcg
    12661 aagacctggt cgcattggcc ggacgatcga cgccggtcac cacgaaacaa aataccgtca
    12721 gcctgttccg atccaccttt tcccgcatcg accggctgct gaaagaaggc gccattcatg
    12781 tcgggatcga agcggcagat ctgcgattcc ggaaattctc cggcacccat gccaaagccg
    12841 atctgatttt taacgaccgc tccatccggc tcaaccgcct gagcgtcgca caaggcagcg
    12901 gagctatcga gctgaaggct actttgagca ggctggcgcc cggtgagtcg agcccgctga
    12961 tcctggaatc gcacctcgaa aaggtggatc taccccgaat cttcgcctcc ttcaacaatt
    13021 ttggtcagga cgccattgag gcgcagaatc tgaaaggtca gctgaccgcc gacgcccgga
    13081 tgaccgggaa actcaccggc aaagcgaaga tcgtcccgaa cagcctgaaa ggttctgtcg
    13141 gcttcaggat caccgacggc gagctggtcg acttcccgcc catggaaaaa ataaaggtcg
    13201 gtttcctgaa gaaccgggat ctttccgcgg tacgcttctc cgggctggag aacgagctgg
    13261 acgtcgattc gactaccgtt acgatacacc ggatggagat caattcctcc gcctttacgt
    13321 tgtttgccga gggaacctac gatctgaaaa cgggtccgga cataagtctc cagatccccc
    13381 ttcgaaacct gtccgacaac aagtaccaga gcatcccacc cgaaagccgc ggcaatgacg
    13441 gcaaggccgg tcccagcatc cggcttcgcg cgcacaggga cgacgagggt aagctgaaga
    13501 tcacctggga cccgttcaaa agggcgttga aaaaagagaa acgggcatcg cgctcgaagg
    13561 gccgcgaggc gaaatcttcg taaattcgcc acttacgaaa ctttattcca tgccttttaa
    13621 gatcaactct ccctttcctc cgtccggcga tcagccagaa gccatccgcc agctgacgga
    13681 aggcatccaa caaggagagc cataccatac ccttctcggc gtcaccggct caggaaagac
    13741 cttcaccatc gccaacgtca tccagaacgt tcagcgaccg acactggtgc tgacacataa
    13801 caagacgctg gtcgcgcagc tttatggcga gttcaaacaa ttcttcccgg aaaatgctgt
    13861 gggctatttc gtaagctatt acgattatta tcagcctgag gcgtatatgc cggtcagcaa
    13921 cacctatatc gaaaaagacc tttccatcaa cgaggagctc gaaaagctgc gcttgcaggc
    13981 gactacgcag ttgctcagcg gcaggagaga tatcatcgtc gtggcatcgg taagctgtat
    14041 ctatggtatc gggaatcccg cggaattcga gaacggcatc atacgcctcc agcaggggca
    14101 gaccatctca cgtcagggat ttttgcatgc gctggtcaac agtctctaca aacggaccga
    14161 gatagaattc gcacgcggga acttccgcgt caagggagac acggtcgata tcaatctccc
    14221 ttatgtggac tatggctatc ggatcagctt ctttggcgac gagatcgatt cgatcgaaag
    14281 cttcgacatt tcttcgggta agcgtatcgt cgcgatggat aacgcggcca tttttccggc
    14341 caacctctac ct
//
LOCUS       DASYSV010000172         6847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1185613, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000172 DASYSV010000000
VERSION     DASYSV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185613"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VGQ51_05240"
     CDS             <1..649
                     /locus_tag="VGQ51_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV9035998.1"
                     /translation="GETAIYPPVDMVFDPRLNLLDYQISPAELLQNILWKVEAVSLRV
                     VKELKSPGIFAVELFVTRQGDVYVNETAPRVHNSGHHTIEANYSSQFDMLWRVILGYP
                     LGNTDAILPGAIVNLIGEEGCEGEAYYDGLDEVLHIDNVFVHIYGKTETRAGRKMGHV
                     TIVSREKQDLIYKAHKIKNTLKVISKEAAAANAAAAALALQQPPATEMRDRSIGR"
     gene            674..1192
                     /gene="purE"
                     /locus_tag="VGQ51_05245"
     CDS             674..1192
                     /gene="purE"
                     /locus_tag="VGQ51_05245"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV9035999.1"
                     /translation="MNKPQVGIIMGSDSDLSIMKAAADMLDEFSIPFEMTVVSAHRTP
                     LRMVQYAVQAADRGLKVIIAGAGGAAHLPGMVASLTTLPVIGVPIKSSNSIDGWDSVL
                     SMLQMPGGVPVATVALNGGKNAGILAGSIIGAFDASIREHVSEYKKKLEQEVLSKVDK
                     LKEEGWGNAFDK"
     gene            1285..2094
                     /locus_tag="VGQ51_05250"
     CDS             1285..2094
                     /locus_tag="VGQ51_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEV9036000.1"
                     /translation="MFHLACHSNRRAPLLLPAALLACTSIFAQQYDSTDHRATEVYSP
                     VPPVVTPAAQCAGAPSDAVILFDGSNEDQWVRVSDGKPADWIVADGILTVNKQSGNIQ
                     TKQCFTNYQLHLEFRIPANISGSDQARGNSGLFLASTGKGDEGYELQILDNYNNKTYT
                     NGQVGAVYKQGIPLANPSRKPGEWSSYDVVWTAPVFNSDGSLASPAKVTAFLNGVLVQ
                     NNFELKGVTRYIGKPFYKPHGPCPVKLQAHGDKSEPISFRNIWIRPLKALE"
     gene            complement(2145..4601)
                     /locus_tag="VGQ51_05255"
     CDS             complement(2145..4601)
                     /locus_tag="VGQ51_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036001.1"
                     /translation="MLRSFFTIAWRNLRKNRTYALINVSGLSLGIACGLLIYALVGYH
                     LSFDDFHAGKDRIYRVVTEFHDEIADYSQGVPSPLGKAFRKDFDYAEAVARVVDFNND
                     LITFADGSGGGNLGGGSNGDRGANGGVGGGVIRKFEEPKGIAYAEPAFFGIFNFPLLE
                     GDRTTALQHPGQALITERLAKKYFGEAHAAMGRVIRIDNVTDFAIVGILKDIPVNSDR
                     QQEIYLSYDDVKDKNRRISSDSSWGSIYSQCMCFVRLRPSVTVAQADSGLARMGRTYQ
                     KGRDAQTTIFRLQPLSDIHFNPDFDGYVDKKYLWALFFIGVFIVLTACVNFINLATAQ
                     ALNRCTEVGIRKVLGGLRTQLFWQFVLETAFIAVLAAVIAYGLSLAALPAVNSLFRSA
                     IGFDRLGSLSTLAFLLGLLVVVIFLSGSYPGVVLSRFRPAAALKSRLSQKETGSFSIR
                     RALVVTQFAISQLLIIGTIVIAAQLHFVKDIDMGFDKDAIVMVPLPGHDPVKLRSMRT
                     QLEQIAGVRSVSLCYSAPASESNNSTNLRFDHRAEDEHWEVNTKKADDRYLRTFGLKL
                     VAGRDLFPADTAREFVINETMVRRLDLASATQALGRSLRINKVTGPIVGVVRDFHNSS
                     LRSSVAPIAIFSDINNYDRCAVKITPAHIHSSLAAIEKIWNGAFPDYLYSYRFLDDSI
                     AAFYEFDTSLMNLIGLFAVIAVFIGCLGLYGLTAFMAVRKTREIGVRKVLGAGIPGIL
                     WLFGREFSRLVLVAFLIAAPAAWLIMHQYLQDFKYRITLGPGVFALAMGVTMVIVVLT
                     VGWRSMRAALANPVRALRSE"
     gene            complement(4639..6531)
                     /locus_tag="VGQ51_05260"
     CDS             complement(4639..6531)
                     /locus_tag="VGQ51_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEV9036002.1"
                     /translation="MRILPFFFLLLAAGPLHSQSPIPPPSIAVDQLGFYANGPKYAVV
                     TGPAAGGTFLLVTVGSASPSGSSGVDTVFVGHLGPEMASANSSVSTRLADFSGFHRVG
                     LYRVVVPGFHNSYPFAIGEGVYSRLTAAALKGYYYQRSAMTLTPEFAGKWARQAGHPD
                     TAVLIHPSAAGPERPAGSLISATGGWYDAGDYNKYVVNSGITMGTLLDAYEDFSGYFD
                     TLRTNIPPVGVEGTPGSHSTRVLTSANFTAAHRIPDILNEVLYNLRWMLEMQDPADGG
                     VYNKCTNAAFDGMVMPGVTKTPRYVVQKGTAATLDFAAVMAQAARIFQKFPGRLPGLA
                     DSCRTSSVRAWQWAMQHPDSVYDQNAMNRKFTPPVTTGGYGDYFFGDERYWAAAELLI
                     TTGDTQYLAAVNQGIGAPVVLPTWAYVKTMGDFSLLRHRAGLSALLGVGLDSLRGKML
                     RTADAYTDREAGTAFHTVMGEKRADFVWGSNSVAANEGMLLINAWLLQHKQAYLDGAL
                     SNLDYLLGRNATGYCFVTGLGSHSPMHPHHRPSIADGIDQPVPGLLAGGPNGGRQDGQ
                     HYIYLEPETSYLDNDQAYASNEIAINWNAPLVYLSGGLEALQYSSGYSQHDRLAPSMQ
                     VAAPHK"
     gene            6665..>6847
                     /locus_tag="VGQ51_05265"
     CDS             6665..>6847
                     /locus_tag="VGQ51_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV9036003.1"
                     /translation="MTKVADILTHKGGARITSVTPETTVLEALRIMAEQNIGSVMVLE
                     GSKYLGIMTERDYSRKV"
BASE COUNT         1661 a         2067 c         1788 g         1331 t
ORIGIN      
        1 aggcgagacg gccatctatc cacccgtgga catggtcttc gatccccgtc tcaatctgct
       61 ggactatcag atcagcccgg ccgagctgct tcagaatata ctctggaagg tggaggccgt
      121 ttcgctccgc gtggtcaagg agctgaagag tccgggcatc tttgcggtgg agcttttcgt
      181 gacccgccag ggcgatgtat atgtcaacga aaccgcgccg cgcgtacata atagcggtca
      241 ccatacgatc gaggcgaatt acagctcgca gttcgatatg ctgtggaggg tgatcctcgg
      301 ttatccgctg ggtaacaccg acgccatcct acccggggcc atcgtcaatc tcatcgggga
      361 agaaggttgc gaaggcgagg cgtattatga cggtctggac gaagtgctcc acatcgacaa
      421 tgtgttcgtc catatctatg ggaaaacgga gacgcgggcc ggccgcaaga tgggccatgt
      481 gacgatcgtc agccgtgaga aacaagacct gatctataag gcgcataaga taaagaatac
      541 cctgaaggtc atcagcaaag aggccgcggc ggctaacgcc gccgcggccg cactcgctct
      601 gcaacagccg ccggcgaccg aaatgcggga caggtccatc gggcgctagg cccccccgca
      661 taaaaagaac caaatgaaca aaccacaggt aggtatcata atgggcagcg acagcgacct
      721 atcgatcatg aaggccgccg ccgatatgct cgacgaattc tcgatccctt tcgaaatgac
      781 ggtggtctcg gcacaccgta ccccgctccg gatggtccaa tatgcggtac aggctgctga
      841 tcgcgggctg aaagtcatca tcgccggcgc cggcggagcc gcccacctgc ccggaatggt
      901 cgcctcgctc actacgctcc ccgtaatcgg cgtacccatc aagtcctcca actccatcga
      961 cggctgggac tcggtccttt ccatgctcca gatgcccggc ggagttccgg tggctaccgt
     1021 agccctcaat ggcgggaaga atgccggtat cctggcaggg tccatcatcg gggccttcga
     1081 tgcctccatt cgcgaacacg tatccgaata caagaaaaaa ctcgaacagg aagtcctcag
     1141 taaggtcgac aagctgaaag aagaaggatg gggaaatgca ttcgataaat aactgcccgc
     1201 ctcaccccat tctcaacgaa ctgcccgtta cggcggatat gcgaaaaatg tcctaaattg
     1261 atcgctcatt aattctttta ccgtatgttc catcttgctt gtcactctaa caggagggca
     1321 ccgctgctcc tgccggctgc cctgctcgcc tgcacttcca tttttgccca gcaatacgat
     1381 tccacggatc accgggcaac cgaagtctat agccccgtcc ccccggtagt cacaccggca
     1441 gcgcaatgcg ccggcgctcc ttcggacgcc gtcatcctct tcgacggctc gaacgaagat
     1501 caatgggtac gcgtttccga cggcaaaccc gccgactgga tcgttgccga cggcattctt
     1561 actgtcaata aacaatcggg aaatatccag accaaacaat gctttaccaa ttaccaattg
     1621 catctcgagt tccgcatccc ggcgaatatc agcggctcgg accaggcccg cggcaacagc
     1681 ggtctcttcc tggcttccac cggcaaggga gacgaaggat acgagctgca gatcctcgac
     1741 aactataaca acaagactta tacgaatgga caggtcggcg ccgtctataa acaaggtatc
     1801 ccgctggcca atcccagccg caaacccggg gaatggtcct cttacgatgt agtatggaca
     1861 gcacccgttt tcaattcaga cggcagcctc gcctccccgg ccaaagtgac ggccttcctc
     1921 aatggcgttc tcgttcagaa caatttcgag ttgaaaggag tgacccgcta tatcggcaaa
     1981 cctttttaca aaccccatgg cccctgcccc gtcaagctac aggcccatgg cgataagagc
     2041 gagcccatca gcttccgcaa tatctggata cgccccctga aagcgttgga atagcgcccc
     2101 ttccgcactg aatttctttc aaagccccca cacgggggct ttttctattc gctacgcagc
     2161 gccctcaccg gattagccag cgctgccctc atcgatctcc atcccaccgt aagcaccaca
     2221 atgaccatcg tcacccccat cgccagcgca aagacgcccg gccccagtgt tatccggtat
     2281 ttgaaatcct gcaggtactg gtgcatgatc agccaggcgg cgggagcggc gatcaaaaat
     2341 gcgaccagca ccagccggga gaactcgcga ccgaaaagcc agaggatacc gggaataccc
     2401 gcccccaaca ccttccggac gccgatctcc ctggtcttgc gcacggccat gaaagccgtc
     2461 agaccataga gccccagaca gccgatgaaa acggcgatga cggcaaacaa cccgatcagg
     2521 ttcatcaaag aagtatcgaa ttcataaaag gccgcgatgc tgtcatccag gaaccgatag
     2581 gaatacagat agtccgggaa ggccccgttc catatctttt cgatggcggc caaactgcta
     2641 tggatatgcg caggtgtgat cttcaccgca caacggtcat aattgttgat gtccgaaaag
     2701 atggcgatgg gcgcgacact gcttcgcagc gaagagttgt ggaaatcgcg gaccaccccc
     2761 acgatgggtc cggtaacttt attgatcctg agcgatctcc ccagagcctg tgtagcggaa
     2821 gcgagatcca gccgccggac catcgtctca ttgatcacga actccctcgc ggtatccgcg
     2881 gggaacaggt ccctgccggc aaccagcttt aatccgaagg tccgcaggta tcggtcgtcg
     2941 gccttcttcg tattcacctc ccagtgctcg tcctcggccc ggtggtcgaa tcgcagattg
     3001 gtcgaattgt tgctttctga ggccggcgca ctataacaca acgatactga ccgaaccccc
     3061 gctatctgtt ccagctgtgt gcgcatagaa cgcagcttca ccggatcatg acccggcagc
     3121 gggaccatca cgatggcgtc tttgtcgaat cccatgtcta tgtcctttac aaaatgcagt
     3181 tgtgcggcaa tgacgatcgt cccgatgatc agcaattggg agatcgcaaa ttgcgtgacg
     3241 accagggcac ggcggatcga aaagctgcct gtttcctttt gcgagagcct gctcttgagc
     3301 gcagctgccg gacgaaaacg cgacaaaacc accccgggat agctcccgga cagaaaaatg
     3361 acgaccacta acaatcccaa caggaaagcc agcgtagaca ggcttcccag ccggtcgaaa
     3421 ccgatggcgc tgcggaaaag gctattgacg gccggcaatg cggccagcga cagtccatag
     3481 gcgatcaccg ccgccagcac cgcaatgaac gcagtctcca gtacaaactg ccagaacaac
     3541 tgggttcgca aaccgcccaa caccttgcgt atccccacct ccgtgcaacg attcaacgcc
     3601 tgcgccgtcg caagattgat gaaattgaca caagcggtaa gcacgatgaa gaccccgata
     3661 aagaacaagg cccataggta tttcttgtcg acatagccgt caaaatcggg attgaaatgg
     3721 atatcggaga gaggctgcag ccgaaagatg gtcgtctgcg catcccggcc cttctgataa
     3781 gtcctgccca tccgggccag cccgctgtcg gcctgtgcca cggtgaccga cggtcgcaac
     3841 cgcacaaaac acatacattg gctatagatg cttccccagc tgctatcgct agagatcctc
     3901 cggttcttgt ccttcacatc gtcgtaggag agatagatct cctgttgccg gtccgaattg
     3961 acgggaatat ccttcaggat accgacgatg gcaaaatcag tcacgttgtc tatccggatc
     4021 acgcggccca ttgccgcatg cgcctccccg aaatactttt tggccagcct ttcggtgatc
     4081 aatgcctgac cgggatgttg cagcgccgtg gtcctgtcgc cttcgagcag cgggaaattg
     4141 aatataccga aaaaagcagg ctccgcatat gcaatgcctt tcggttcttc gaacttgcgg
     4201 atcacaccgc cacccacacc gccattcgca ccccggtcac cattcgaacc cccgccgaga
     4261 ttcccacccc cgctcccgtc ggcgaaagtg atcaggtcat tattgaagtc gaccacccgc
     4321 gcgacggcct ccgcataatc gaagtccttt ctgaaagcct tcccgagcgg actgggtacg
     4381 ccctgcgaat agtcggctat ctcgtcatgg aactcggtga ccacgcgata gatccggtct
     4441 ttgccggcat ggaaatcgtc aaagctgaga tgataaccta ccagcgcgta gatcagcaaa
     4501 ccgcaggcaa tgcccagcga caaacccgac acattgatca atgcataggt cctgttcttc
     4561 cggagattgc gccaggcaat ggtaaaaaaa ctcctaagca taactgattg ttcattttat
     4621 tgagttgccc gtatcccttc acttatgcgg tgcggccacc tgcatcgagg gagcaaggcg
     4681 atcatgctgc gaataacccg aactatactg caatgcctcc agtccgcccg aaagatagac
     4741 cagcggcgca ttccagttga tagcgatctc gtttgacgca tatgcctggt cgttgtcgag
     4801 ataggatgtc tccggttcca ggtatatata atgctgaccg tcctgccggc cgccattggg
     4861 tccgcccgcg agtaggccgg gcacgggctg atcgatgcca tctgcaatag acggccgatg
     4921 atgcggatgc attggcgaat gactgcccag accggtgacg aaacaataac ccgtagcatt
     4981 gcggccgagc agatagtcca ggttggaaag cgccccgtcg agataagcct gtttgtgttg
     5041 caacaaccag gcgttgatca gcaacatgcc ttcattagca gcaaccgaat tgctgcccca
     5101 gacaaagtcc gcacgcttct cccccatcac ggtatgaaaa gcagtgcccg cttcccggtc
     5161 ggtataggca tccgccgtcc gcaacatctt cccgcgcaaa gagtcgagtc ccacgccgag
     5221 caatgcggat aaccccgccc ggtgcctcaa cagggaaaag tcccccatcg tcttgacata
     5281 agcccatgtc ggcaggacca cgggagcgcc gataccctgg ttcacagcgg ccaggtattg
     5341 cgtgtccccg gtagtaatga gtaattccgc cgcagcccag taacgttcgt cgccaaaaaa
     5401 ataatcgccg tagccgccgg tggtaacggg tggcgtgaat ttccggttca tcgcattctg
     5461 atcataaacg ctgtcgggat gttgcattgc ccattgccag gcgcggacag acgatgtgcg
     5521 gcaactgtcc gccagccccg gtagccggcc cggaaatttt tggaaaatgc gggccgcctg
     5581 cgccatcaca gctgcaaaat ccagcgtagc agccgttccc ttttgaacca cgtatcgtgg
     5641 tgtcttcgta acccccggca tgaccatccc atcgaatgcg gcattggtgc atttattgta
     5701 aacgcctcca tcggcagggt cctgcatttc cagcatccag cgaagattat acagtacttc
     5761 attgaggata tcgggaatac ggtgtgccgc ggtgaaattc gccgacgtca acacccgcgt
     5821 cgaatgagat cccggcgtcc cttcgacacc caccggcggt atattcgttc tcagggtgtc
     5881 gaaatacccc gaaaaatcct cataagcgtc gagcagggtc cccatggtga tgcccgagtt
     5941 gaccacgtat ttgttatagt ccccggcatc ataccagcca cctgtagccg aaataaggct
     6001 accggccgga cgttcgggtc cggcggcgga aggatggatg agcactgcag tatccggatg
     6061 accggcctga cgcgcccact tacccgcaaa ttcgggtgtc agcgtcatcg ccgatcgctg
     6121 gtaatagtac cccttcaacg ccgccgccgt cagccgggaa tataccccct ccccaatggc
     6181 aaatggataa gaattgtgaa aacccggcac gaccaccctg tataacccga ccctgtgaaa
     6241 accactgaaa tcggcgagac gcgtcgacac cgacgagttg gcggacgcca tttccggccc
     6301 gagatgcccg acaaaaaccg tatccactcc cgaagacccg gacggagaag cgctccccac
     6361 tgtgacgagc aagaacgtcc cccctgcggc aggacccgtt acgaccgcgt atttggggcc
     6421 attggcatag aagcccagct ggtcgacagc aatagacggc ggcgggatcg gtgattggga
     6481 atgcagcgga ccagcggcca gcagcaggaa gaaaaaaggc aggatgcgca tgataaccgg
     6541 ttgatatggt caaagataag cacacggggg atgggcgccc cctcccggga taaattttga
     6601 acgaaaaagg aaaaaaattg cgtaaattga aaaaacagga ttacctcacc aaaaaatcac
     6661 acgtatgact aaagtagctg atattcttac tcataaagga ggggctcgta tcacctccgt
     6721 gaccccggaa acgacagtgc tggaagccct gcggatcatg gccgaacaga atatcggatc
     6781 ggtgatggtg ctggaaggaa gtaaatacct cgggatcatg accgaacggg actattcccg
     6841 gaaagtg
//
LOCUS       DASYSV010000173         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_415876, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000173 DASYSV010000000
VERSION     DASYSV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415876"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VGQ51_05270"
     CDS             <1..489
                     /locus_tag="VGQ51_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEV9036004.1"
                     /translation="ADGSAAALIVPDKDAGRGLQLKHFYSEIVSRGKKEMTWDVSSTG
                     FRMGLSNYVTDLISTDIAGLVEHALEKGGVQRQEITHWCVHPGGKKILDAIHRSLSFS
                     NGNLDPSYTILRQYGNMSSPTILFVLQHIMEGLSYHESNCIFGAAFGPGLTMETFIAQ
                     TH"
     gene            499..1212
                     /locus_tag="VGQ51_05275"
     CDS             499..1212
                     /locus_tag="VGQ51_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV9036005.1"
                     /translation="MPARLDFSRRSNDTELLDRDDIPFGDIERNMRELDFINTWLGGH
                     SISIAGLKQLARGRRRLSICEIGCGGGDNLRVLYRWCQKQGIEVKVTGIDKNAHCLSV
                     ARETWKGGEAEWVHSDYRDARFTIDKPDVIFSSLFCHHFNNEELIFQLRWMEEHAGLG
                     WFINDLHRHPVAYHSIRWLTAGFSTSYLVKHDAPLSVLRGFRRKEWESLLGQALTGSC
                     QLKWKWAFRWLVVGRKRYV"
     gene            1205..2329
                     /locus_tag="VGQ51_05280"
     CDS             1205..2329
                     /locus_tag="VGQ51_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV9036006.1"
                     /translation="MYDVIIAGGGLAGLALSIQLVKAGYGVVLFEKETYPYHKVCGEY
                     ISLESWNFLQELGLPLSDWNLPIIRTLMLSAPDGCSLRQELPLGGFGISRYKIDAALA
                     NIARSAGVQLYESVRVTDIRFERSVFRVRTSAGDFESRVACGTFGKRSNLDVRWKRAF
                     IRKRSDKLNNFVGVKYHVRTEYPPDQIGLHFFRNGYCGISKVEEDRYCLCYMTAAENL
                     SVSGNSIPEMEKNILRKNPFLEEVFASAVPLFEQPVTISQISFEKKTQVENHILFVGD
                     AAGMVPPLSGNGMSMALHGSQIACRCIESFLKDEIARYEMEQEYIDRWNSQFGKRLWM
                     GRSLQRWLGSERGANFLVHSLKPFPKLVSFLIRQTHGQPF"
     gene            2390..>3084
                     /locus_tag="VGQ51_05285"
     CDS             2390..>3084
                     /locus_tag="VGQ51_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036007.1"
                     /translation="MICTLCLEGTAQDYQGIQGSPYAGSLGVANNPASILSTPYKWDI
                     TIFSAQLKTTTNAITVTNLSYLHPFIHRRDSLDYYWNSGYMRRYGAVNYNLHLLNVRL
                     ALNRKQAISFGANLKSYGAVRMSPFAYIDTIGNFNQFFAMNYGTSSYEGEAVSSTWLE
                     LYGTYSKTLIDDEVGRLNGGVTLKLMRGISGAYAKFSGGGVSSTYINSQLVYTLAKGD
                     VQYGYSSNYDSWNS"
BASE COUNT          727 a          727 c          889 g          741 t
ORIGIN      
        1 gcggacgggt cggcggcggc cctgatcgtg ccggataagg atgccgggcg cgggctgcag
       61 ctgaaacatt tctattcgga gatcgtatca cgagggaaga aggaaatgac ctgggatgtg
      121 tcttcgacgg gtttccgcat gggtctgtcc aactatgtga cggatctgat cagcaccgat
      181 attgccggcc tggtcgaaca tgcactggaa aagggcgggg tgcagcggca ggagatcacg
      241 cactggtgcg tgcatccggg tggcaagaag atcctcgacg ctattcatcg gagtctttct
      301 ttctccaacg ggaatctgga cccgagctat acgatccttc gacaatacgg gaatatgtca
      361 tctcccacga tactctttgt cctgcagcat atcatggagg ggctttcgta tcatgaatcc
      421 aattgtatct tcggggcggc attcggcccc ggtcttacca tggaaacctt catagcacaa
      481 acccattaat ttcgtcttat gccggcgaga cttgattttt caaggcgaag caatgacacg
      541 gaattgctgg acagggacga tatccctttc ggggatatcg agcggaatat gcgggagctg
      601 gactttatca atacctggct gggcggacat tcaatcagca tcgcaggatt gaagcagttg
      661 gcccggggcc ggcggagact cagcatttgt gagatcggtt gcggtggcgg ggataatctc
      721 agagtgctat accgctggtg tcaaaagcag ggcatcgagg tgaaagtcac gggcatcgat
      781 aagaatgctc attgtttatc ggtagccagg gaaacatgga agggtgggga agccgagtgg
      841 gtgcattcgg actacaggga cgcacggttc acaatagaca aaccagatgt aattttctca
      901 tcgctttttt gtcaccattt taacaatgaa gagcttatct ttcagctaag atggatggag
      961 gagcatgccg gattgggttg gttcatcaat gatctgcacc gccatccggt ggcttatcat
     1021 tcgatccggt ggctgacggc tggattttct acctcctacc tggtgaagca tgacgctcct
     1081 ctttccgtgt tgcgggggtt ccggcgcaag gagtgggaaa gcttgttggg gcaggccctg
     1141 accggatctt gtcaattgaa gtggaaatgg gctttccggt ggctcgtggt cgggagaaaa
     1201 cgatatgtat gacgtcatca tagcaggcgg aggactggcc ggactggcac tttccatcca
     1261 attggtcaaa gccggctacg gggtagtcct ttttgaaaag gaaacctatc cttatcacaa
     1321 agtctgcggc gaatatatca gcctggaaag ctggaatttc ctgcaggagc tgggattacc
     1381 cctcagcgac tggaatctgc ccatcatccg gacgctgatg ctttctgcac ccgatggctg
     1441 ctcgctgcgg caggaattgc cgcttggcgg attcggtatc agccggtata agatcgatgc
     1501 cgcgctggcc aatattgcgc gatcggcggg cgtgcagcta tatgaatcgg tgcgcgtcac
     1561 ggacatccga ttcgaacgat cggttttcag ggtccggacc agcgccggcg atttcgagtc
     1621 gcgggtagcc tgcggtacct tcggcaaaag aagtaacctg gacgtccgct ggaaacgggc
     1681 tttcatccgc aagcggtcgg ataagctcaa taattttgtc ggggtaaaat accatgtgcg
     1741 caccgaatac cctcccgacc agatcggtct tcattttttc aggaacgggt attgcggtat
     1801 atcgaaggtc gaagaggacc gttattgcct ttgttatatg actgctgccg agaacctgag
     1861 cgtcagcgga aattcgatcc cggaaatgga aaaaaatatt ttgcggaaaa atccgttcct
     1921 ggaagaagta tttgcatcgg cggtccctct cttcgaacag ccggtgacga tctcacagat
     1981 cagtttcgaa aaaaagacac aggtcgagaa ccatatcctg ttcgttgggg atgcagcggg
     2041 gatggtgccg cctttgtcag gcaatgggat gagcatggcg ctgcatggga gtcagatcgc
     2101 ctgtcgttgt atagagagtt ttctgaagga tgaaatagcc cgttacgaga tggaacagga
     2161 atatatcgac cggtggaata gccaattcgg caaaaggttg tggatgggta gatcgttgca
     2221 acgctggttg gggagtgagc gcggggccaa tttcctggta cattcattaa aacctttccc
     2281 aaaactggtt tcatttttga tcagacagac ccatggacag ccgttctaag acggcacata
     2341 tcaattactt ctcatgaaat gtcgcgcaaa aggagttcta attatcggtt tgatctgtac
     2401 cctgtgcctg gaaggaacgg cgcaggatta tcagggcata caaggatcgc cgtatgcggg
     2461 atcgttgggc gtagccaata acccggcctc gatcctgagc acaccctata agtgggacat
     2521 aacgatcttc tccgcccagc taaagactac gaccaatgct atcacggtca cgaatttatc
     2581 ctatctccac cccttcatcc accgcaggga cagtctcgat tattactgga acagcgggta
     2641 tatgcgtcgg tatggcgccg taaattacaa cctgcacctt ctgaatgtcc gactggcgct
     2701 gaatcgcaag caggccattt cctttggggc caacctgaag agctatgggg ccgtgcggat
     2761 gtcgccgttc gcttacatcg atacgatcgg gaatttcaac cagttctttg caatgaatta
     2821 cggcacttcg tcatatgaag gtgaagcggt gagcagtacg tggctggagc tctatgggac
     2881 ctatagtaag acgctgatcg atgatgaggt gggccggctg aacgggggcg taacgctaaa
     2941 actcatgcgc ggtatctcgg gagcctatgc caaattttcg ggtggtggtg tgagcagcac
     3001 ctacattaac tcccagctgg tttataccct ggcaaaaggc gacgttcaat atgggtattc
     3061 ttcgaactac gattcgtgga attc
//
LOCUS       DASYSV010000174         7296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1827580, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000174 DASYSV010000000
VERSION     DASYSV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827580"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VGQ51_05290"
     CDS             <1..279
                     /locus_tag="VGQ51_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9036008.1"
                     /translation="EAAASRIRPILMTSVAMVVGMIPMASGLGEGGDQAAPLGRAVIG
                     GLIASTFAALFLLPLIFAWVQGKTGTESVSMDPSDKESKHYIKSLYEK"
     gene            269..1402
                     /locus_tag="VGQ51_05295"
     CDS             269..1402
                     /locus_tag="VGQ51_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9036009.1"
                     /translation="MKNNLVAGLAIVVCSTGILTGCSNTDGEKSAKGDSGVSAAPAHS
                     DSAAAGTATFILKKGRFSTDLHVPGELIAFQQVDVYAKVSSFVKKLYVDVGSEVREGQ
                     LLVTMEAPELESQLASAESRVKSQEAIYIASKSNYDRIVETSKTPGTISQNDIDQADA
                     RQKSDQAQMDAAKAAYNEVAATLKYLEIRAPFSGVISSRNVNPGAYVGPSGKGSELPM
                     FTLQEQKHMRLVISVPEAYTGYLSDKDKVKFTVKALPTRQFTAQVRRLAGALDNHLRA
                     ERTEMDVYSEDKTLLPGMVAEVDLPLPDRDSSFVVPTSAVVDGTERLFVIRVGDDRKA
                     EWVDVQTGRSQGGKIEVFGKGLKEGDRLVTSATEEFRDGAVVR"
     gene            complement(1405..2796)
                     /locus_tag="VGQ51_05300"
     CDS             complement(1405..2796)
                     /locus_tag="VGQ51_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEV9036010.1"
                     /translation="MAILKMGHRSPTVPAVLIPRVAPHSLIAPTRPFPQHTPYLGGAI
                     LPDHIGRSAMDDTVRSFYRQWKQHYILPGCAPDEKYVWFEGTSGRNICVSEGQGYGMM
                     IVALMAGFDSTAQETFDDLFRYVQSHPSTTSPNLMAWTQTRYCRSVDGGTATDGDMDI
                     AYALLLADAQWGRNSNIPYRQKAIERIEAIRREEINPVTYTVMEADAETPGSRDYFDM
                     RPSDFMPDHIRAFREATGDPRWDSVLNNNYLLLRYMQDTYSPDAGLVPDFIKNIRLRP
                     GQGYDGAPSAPRGDGDETMTATEEEVSATPAQPNYLESKYDGKYNFNACRVPWRIAAD
                     YLVSGDKRAADFLSRINPWIRQTTSGNPDNISAGYNLSGEDLPHRYFEALCFICPFAV
                     AAMTDAHHQEWLNRLWDYIVGFKMKDFDYYDNSIKMIDLIILSGNYWSPVAPSADQAS
                     VPGAIRQSASSAR"
     gene            2986..4026
                     /locus_tag="VGQ51_05305"
     CDS             2986..4026
                     /locus_tag="VGQ51_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036011.1"
                     /translation="MKTSLSLCLSLIAFSFVVVFACTPAGIPATQLGNWVQAAAIGAY
                     PRSNSTCFTIGDDAYVGLGFNESVGGTGRLNDFWTFNVDSGWTQIEDFPGAPRSNAAG
                     FSIGNYGYAGTGWDTYSIFSDFYQYDPAQNVWSRKADFPGRPRYDAVGFGVQGKGYIG
                     TGFNVYWMNDFYGYDPVQNSWSVTPGTSGNFSKRRGAAAFVYMDKAYIVTGSNSGGMV
                     RDFWRFDPSQPEEWYRLSDIINDSAATFDDGYSDIEREFATVFVNGTQAFLTTGDNSR
                     SSMATTTWAYDIPSDRWSRRTSYPRASRNGAVAFTVSGRSFLGTGNTGSNGTFDDFDE
                     FQPLVAFDSNDY"
     gene            complement(4051..6333)
                     /locus_tag="VGQ51_05310"
     CDS             complement(4051..6333)
                     /locus_tag="VGQ51_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV9036012.1"
                     /translation="MRKASIIRIFWILFFTGLLGFIGIIALIAAGAFGQLPSLHELEN
                     PSLVLASEVYAADGTSMGKYYTAKGNRLDVSYKDISPNVIHALVSTEDERFYEHSGVD
                     AKAVLRAILLFGRDGGGSTITQQLALNMFNGERSHNRISRVIQKLKEWVIAIELERNF
                     TKEEILTLYLNDVSFSDNVYGIRGAARTFFRKDPDSLSVDEAAVLVGMVNNPSFFNPR
                     TNPRAATDRRNVVLSRMVSNHYLTEQDAAVLKAKPIDLSHYRKLDENNGLAPYLRDIL
                     RGDLKKWCKEHINPATNKPYNLYQDGLKIYTTIDPRMQAYADEAVNQQMPVLQAMLDG
                     QRNIHNGSVWGPHMNILDAAMRNSERWRDEEDAGLSESEIRYSFTVPVKMKVFAWNAA
                     REKDTVMTPLDSIKYHRQMLQTGFMVMDPFTGAVKAWVGGIDFRTYKFDHVNVGTKRQ
                     VGSSIKPFLYSLALEDFNFTPTTQCPAIQQFFPEYNAYVPAKSGSHWEPGPYPMAYGL
                     AWSINEVAAYLMKSFGPQGPQRFAEFLKQIGIPTTVQPYPSMALGACELSLYEMLGGY
                     TMFPSEGIHTDPYYISRIEDKHGNLLASFIPSHKEVLNQATAYTMTRMMQGTCDFGTA
                     KGLRERLGLAQMGGKTGTTNDNADTWFMGYTPQLLAGAWVGCDDRFIQLEGRSSDGGH
                     IARPIWENFFRLALADKSLGLNRAALFAQPDSVNAALQYDYNGQYEKAPPPGAEGTDQ
                     GNGNAGQYQDSTKAKPGNNY"
     gene            complement(6366..7079)
                     /locus_tag="VGQ51_05315"
     CDS             complement(6366..7079)
                     /locus_tag="VGQ51_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9036013.1"
                     /translation="MSLRCLIVDDEPNAVNLLEMFIRQNTDWDIHGKCYDAREALAFL
                     KSHPVDLIFLDINMPHLTGMEMAPLLPPQTSIVFTTAYSEYAADSYGFPTIDYLLKPI
                     TLKRWYAAMQKIEAHFARPEPPAQSATMPPDDEYFFIKSGRMLHKVLLKDILYFEGDK
                     EYARLVTPTQKLLLYRRLKDIEEQLSASFARVHNSYIVNIARLDSIRDNHVFIGAKQI
                     PISEKFREPFMLRIRERVF"
     gene            complement(7076..>7296)
                     /locus_tag="VGQ51_05320"
     CDS             complement(7076..>7296)
                     /locus_tag="VGQ51_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036014.1"
                     /translation="GNSISLSLTQQDGWLLFATENAIPAEPPAASKPGFGLRNLEKRL
                     TLLYGDRFTLSTIRTETHFQAFLKIPLI"
BASE COUNT         1535 a         1652 c         2385 g         1724 t
ORIGIN      
        1 gaggctgcgg cctcgcgtat caggcccatt cttatgacca gtgtcgccat ggtggttggg
       61 atgataccga tggcatcggg tctcggtgag ggtggcgatc aggcggcacc gttaggacgt
      121 gcggtaatcg gcgggttgat cgcgagcacc tttgctgcgt tgttcctgtt gccactgatc
      181 tttgcgtggg tgcagggtaa gacgggtacg gagtcagttt ccatggatcc ttctgataaa
      241 gaaagtaaac actatataaa gtcattatat gaaaaataat ctggttgcgg ggttggcgat
      301 agtggtgtgt tcgaccggga tattaaccgg ctgctcgaat acggatgggg aaaagagcgc
      361 aaagggggac agcggtgtgt ctgcggctcc ggctcattcg gatagtgccg ctgcgggaac
      421 ggccacattt atattgaaga aggggcggtt ctccaccgac ctgcatgtgc cgggcgagct
      481 gatcgccttt cagcaggtgg atgtctatgc gaaggtgagc agttttgtga agaaattgta
      541 tgtggatgtt ggatccgagg ttcgggaagg tcagttgctg gtgacgatgg aggccccgga
      601 gctggagagt cagttggcca gcgcagagtc ccgtgtcaag tcccaggaag cgatctatat
      661 cgccagcaaa tcgaattatg accggatagt agagacgagc aagacgccgg gcacgatctc
      721 gcagaatgat atcgatcagg cggatgcgcg gcagaagtcc gaccaggcgc aaatggatgc
      781 ggcaaaggcg gcttacaatg aagtagcggc aaccctgaag tatctcgaga tcagggctcc
      841 gttcagcggt gtcatcagtt cgaggaacgt caacccgggc gcctatgtcg ggccttcggg
      901 aaaaggatcg gagttgccga tgttcacctt gcaggagcag aagcatatgc ggctggtgat
      961 ctccgtaccg gaggcgtata cggggtatct gtccgataag gacaaggtga aatttaccgt
     1021 gaaggcgttg ccgacccggc aatttaccgc acaggtgcgc aggctggcgg gggcgttgga
     1081 taatcatttg cgggcagaga ggactgagat ggatgtatac agtgaagaca agaccttgtt
     1141 gccggggatg gtcgcggaag tcgatctgcc gttgccggac agggatagta gttttgtcgt
     1201 tccgacttcg gcggtggtcg atgggacgga gcggttattc gtgatccggg ttggcgatga
     1261 tcgtaaggcc gaatgggtgg atgtgcagac cgggagatcg caggggggaa agatcgaagt
     1321 gttcgggaag gggctgaagg aaggcgaccg gctggtgacg agtgctacgg aggagttcag
     1381 ggatggggcg gtcgtaaggt aatcttagcg tgcagatgag gccgattgcc ggatcgcgcc
     1441 cgggacggag gcctggtccg cggatggcgc taccggtgac caatagttgc cggacaggat
     1501 gatcagatcg atcattttga tgctgttgtc atagtaatcg aaatctttca ttttaaatcc
     1561 gacgatatag tcccatagcc tgttcagcca ctcctggtgg tgggcgtccg tcatcgctgc
     1621 aaccgcgaag gggcagatga agcagagggc ttcgaaatac cggtggggca ggtcttcgcc
     1681 ggaaaggttg tatccggcgg aaatattgtc cgggttgccg ctggtagtct gccggatcca
     1741 tgggttgatc ctgctgagaa aatcggcggc gcgtttgtct ccggagacga ggtagtccgc
     1801 ggcgatgcgc cagggcacgc ggcaggcgtt gaagttgtac ttcccgtcgt atttggattc
     1861 cagataattg ggttgggcag gggttgctga gacctcttct tcggtggcgg tcatcgtctc
     1921 atcgccgtcg ccgcgcgggg cgctgggtgc gccatcgtag ccctggccgg ggcgaagacg
     1981 aatattctta ataaaatcgg gtaccagtcc ggcatcgggg ctataggtgt cctgcatgta
     2041 gcggaggagc agataattgt tgttaagcac gctgtcccag cgagggtcgc cggttgcctc
     2101 gcggaaggcc cggatatggt ccggcatgaa gtccgagggg cgcatatcga aataatccct
     2161 gctcccgggt gtctcggcat cggcttccat gacggtatag gtcaccgggt tgatctcttc
     2221 gcggcgaatg gcttcgatcc tctcgatggc cttctggcga taggggatgt tgctgttgcg
     2281 gccccattga gcatcggcga gcaggagcgc gtaggcaatg tccatgtcac cgtcggtggc
     2341 ggtgcctcca tcaacgctgc gacagtaccg ggtctgtgtc caggccatga gattggggct
     2401 ggtggtgctg ggatgcgatt ggacgtaacg aaaaagatca tcgaaggttt cctgcgccgt
     2461 ggaatcgaag cccgccatca gcgcgacgat catcatacca tagccctgtc cttcggagac
     2521 gcagatattg cggcccgatg ttccttcgaa ccaaacgtat ttttcatcgg gagcgcatcc
     2581 gggaaggata taatgttgtt tccattgccg gtagaacgag cggacggtat catccattgc
     2641 ggaccggccg atatggtcgg gcaggatggc cccgccgaga tagggcgtgt gttgagggaa
     2701 agggcgtgtg ggagctatga gagagtgagg cgcgacgcgc ggaatgagaa cggcggggac
     2761 ggtcggtgat cgatgaccca tcttgagtat agccaaaccc cacaaaactg cttttatacc
     2821 atgcatggga aaaaattgac aaagtaatgt aaagttaagc aattactatg ccggtgcgaa
     2881 gagagaggtc tgttaacaat tcttaaggga aacattaaca ggacgaatgc atcggagaag
     2941 tgagttgagc gctcttagat ctttctccta aatcaaaact ccaacatgaa aacttctctt
     3001 tcactttgtc tttcattgat cgctttttct tttgtcgtcg tattcgcctg cacgcctgcg
     3061 ggcatcccgg caacacagct gggaaattgg gtgcaggcgg cggccattgg ggcttatccg
     3121 aggagcaaca gcacttgttt caccattggc gatgatgcct atgtcgggct cggattcaat
     3181 gaatccgtcg ggggaacggg tcgtctgaat gatttctgga ccttcaatgt cgacagcggc
     3241 tggacgcaga tagaggactt ccccggcgcc ccgcgaagca atgctgcggg attcagcatc
     3301 ggtaattatg gttatgcagg tacgggctgg gacacctatt ccatctttag tgatttttat
     3361 caatatgatc ccgcgcagaa tgtgtggtcg agaaaggcgg attttccggg gaggccgcgg
     3421 tatgatgccg tcgggtttgg cgtgcaaggg aagggatata tcggcaccgg cttcaatgtt
     3481 tattggatga atgattttta tgggtatgac cccgttcaga atagctggtc ggtgacgccg
     3541 ggtacttccg gtaatttttc gaaacggcgt ggcgcggcgg cttttgtcta tatggacaag
     3601 gcttacatcg tgacgggctc gaacagcgga ggtatggtaa gggatttctg gcgtttcgat
     3661 ccttcacagc cggaggaatg gtatcggttg agcgatatca tcaatgacag tgcggccaca
     3721 ttcgacgatg ggtattcgga tatcgagcgg gaatttgcta cggtctttgt gaacggtacg
     3781 caggcattcc tgacgacggg ggacaatagc cgaagcagca tggccactac cacgtgggcc
     3841 tatgacatac ccagtgaccg gtggagccgg cggacgtcgt atccgcgtgc ttcgaggaat
     3901 ggggcggtgg cttttacggt gagtggaaga agttttttgg gaaccgggaa tacgggtagc
     3961 aacggcacct ttgacgactt cgatgagttt cagccgttgg tggcgttcga ttcgaatgat
     4021 tattaggatg gggcgtttgc ggcgcgcggg ctaataattg ttgcccggtt ttgccttagt
     4081 cgaatcctgg tattggccgg cgttgccgtt gccctgatcg gtgccttcgg cgccgggagg
     4141 cggagctttt tcgtattgcc cgttataatc gtattgcagg gccgcgttga cgctatcggg
     4201 ttgtgcaaag agggcggctc tgttgagccc aagggattta tcggcgaggg ccagccgaaa
     4261 gaaattctcc cagatggggc gggcgatatg gccgccgtcg gaggaccggc cttcgagctg
     4321 gataaagcgg tcgtcgcagc cgacccaggc gccggcgagc aattgagggg tatagcccat
     4381 gaaccaggta tcggcgttgt cgttggtcgt acccgtctta ccgcccatct gagcgagacc
     4441 cagccgttcg cggaggcctt tggccgtgcc gaaatcgcag gtgccctgca tcatgcgcgt
     4501 catggtatag gcggtggcct gattcaggac ttccttgtgt gacgggatga aggaagccag
     4561 caggtttccg tgtttgtctt cgatgcggga aatatagtat ggatcggtgt ggatgccttc
     4621 cgaagggaac atggtatagc cgccgagcat ttcatagagc gaaagctcac aggcgcccag
     4681 cgccatagag gggtagggct gcaccgtcgt cggtatgccg atctgtttga ggaattcggc
     4741 aaatcgttgg gggccttgcg gtccgaaaga tttcataagg tatgcggcca cttcgttgat
     4801 ggaccaggcc aggccatagg ccatggggta gggacccggc tcccaatgcg agccactttt
     4861 cgcaggtacg taggcgttat attcgggaaa gaactgttgt attgccgggc attgtgtcgt
     4921 aggcgtgaag ttaaagtctt caagagccag gctgtacaaa aaaggtttta tcgagctgcc
     4981 cacctgtcgt ttggtgccga cgttgacgtg gtcgaactta taggtgcgaa agtcgatgcc
     5041 gccgacccag gcttttacgg ctccggtgaa ggggtccatg accatgaacc ccgtctgcag
     5101 catctgccgg tgatatttga tggaatccag gggagtcatg acggtatctt tttccctggc
     5161 ggcattccat gcgaatacct tcattttaac gggtacggtg aagctgtaac ggatctcaga
     5221 ttcggagagg ccggcgtctt cttcgtcacg ccatctttcg ctattgcgca ttgctgcatc
     5281 gaggatgttc atatgcgggc cccaaaccga gccgttgtgt atattgcgct gaccgtcgag
     5341 catcgcctgg agaacgggca tttgctgatt gacggcttcg tcggcatagg cctgcatacg
     5401 cgggtcgatg gttgtatata tcttcaaacc gtcctgatag agattatagg gcttattggt
     5461 ggcagggttt atgtgctctt tgcaccattt ttttaaatct ccgcggagga tgtcgcgtag
     5521 ataaggggcc aggccgttgt tctcgtcgag cttgcggtag tggctcaggt cgatgggttt
     5581 tgccttgagg acggcggcat cctgttcggt caggtagtgg ttggacacca tccggctgag
     5641 cactacgttg cgtctgtcgg tagcggccct gggattagtg cgcggattga agaaactggg
     5701 gttgttgacc atgcctacga gtacggctgc ttcgtcgacg gagaggctat cgggatcttt
     5761 gcggaaaaag gtccgtgccg cgccgcggat gccatagaca ttatcgctga aggagacatc
     5821 attcagatag agggtgagga tctcttcttt ggtaaaattg cgttccagtt cgatggcgat
     5881 gacccattcc ttcagtttct ggatgacgcg ggagatgcgg ttgtgcgaac gctcgccatt
     5941 gaacatattg agcgccagct gttgggtgat ggtgctgcct ccgccgtccc tgccgaacag
     6001 gaggatggcg cggaggactg ctttggcgtc gacgcccgaa tgttcataga agcgttcgtc
     6061 ttccgtggaa accagcgcat ggatgacatt gggggagatg tccttatagg acacatcgag
     6121 gcggttgcct tttgcggtat agtattttcc catggatgtg ccgtcggccg catagacttc
     6181 cgaggccagg acgagggatg ggttttccag ttcgtgcaac gagggcagct gtccgaaggc
     6241 gcctgcggcg atgagggcga taatgccgat gaagccgagg aggccggtaa aaaacaggat
     6301 ccagaagatg cggataatgg aggctttgcg cataggtaaa taacgcccaa tttcggtaaa
     6361 aattatcaga atacccgctc ccggatgcga agcatgaacg gttcgcggaa tttttcgctg
     6421 atggggatct gtttggcgcc gatgaataca tggttatccc ggatggaatc gagccgggcg
     6481 atattgacga tataggaatt gtgaacgcgg gcgaaagacg cggagagttg ttcctcgatg
     6541 tctttcaggc ggcggtagag cagcaatttt tgggtggggg tcaccagccg ggcgtattcc
     6601 ttgtctcctt cgaaatacag gatatccttt agcagcacct tatgcagcat cctgccggac
     6661 ttgatgaaaa aatattcatc atccgggggc atggtcgccg attgggcggg cggttcgggg
     6721 cgtgcgaaat gggcttctat cttttgcatc gccgcatacc aacgtttgag ggtaatgggt
     6781 ttcagcaaat aatcgatagt agggaagcca tagctgtcgg cagcatattc ggagtaggcc
     6841 gtggtgaaga cgatgctggt ttggggtggg agcaggggtg ccatttccat accggtgaga
     6901 tgcggcatat tgatatcgag aaagatgagg tccacggggt gggattttag gaaggcaagg
     6961 gcttccctcg cgtcgtaaca tttcccatgg atgtcccaat ccgtgttttg gcggatgaac
     7021 atttcgagga gattgaccgc attgggttcg tcatcgacga taagacagcg gaggctcata
     7081 tcagcgggat ttttaggaag gcctgaaaat gggtttccgt acgaatggtg gaaagggtga
     7141 accggtcacc gtataataaa gttaggcgtt tttccagatt gcgaagaccg aagcccggtt
     7201 tggaagctgc cggaggttcc gcggggatcg cgttctccgt cgcgaagagc aaccagccgt
     7261 cctgttgggt gagcgagaga ctgatgctgt tacctt
//
LOCUS       DASYSV010000175         3645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1056465, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000175 DASYSV010000000
VERSION     DASYSV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056465"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1624
                     /locus_tag="VGQ51_05325"
     CDS             83..1624
                     /locus_tag="VGQ51_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEV9036015.1"
                     /translation="MKKSLLIAAGLLSAGLANAQRVIRADYRDEAGSFSRSYATCVGA
                     GRAAEGLRADWQQQLAMAVKAIGFRYIRFHGLLCDEMHVYTEDKTGRPVYNFQYVDKL
                     YDYLLSIGIRPFVELGFMPPDLASGTKTVFWWKGNVTPPKDYQKWADLIQALVRHWQE
                     RYGREEVEKWYFEVWNEPDLHGFFTGTQDDYFKLYKTTAEAIKSVSPTYRVGGPATSG
                     SKWIREMLQYCRNNQVPLDFISTHSYNTKTVFDEFGVRKRTMLPSGYLWGNVVNTRRI
                     MDSCAYSHLPLHYTEINSSPSSRDPLHDSYQNAAFLLNTLKHTEHAAASMSYWTFTDV
                     FEEAGPGPTPFHGGFGLINLQGLRKPTFFAYQFLHRMGNTALRDTDSCSWVCRSDKGV
                     QTLFWNLHLPFADAPYFNDSLFRLNTPPAPAGTVTVKLDHIPNGRYRVLVYHIGYHHN
                     DVFSAWLEMGSPSDLSGQQQQRLDAIANGAPESSGTVTIDDGTFSRRFDTKENDIYLI
                     QLERI"
     gene            1621..>3645
                     /locus_tag="VGQ51_05330"
     CDS             1621..>3645
                     /locus_tag="VGQ51_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036016.1"
                     /translation="MKRLSLLVLLPLLLGVKAEDVPRMIVFTTLHSVRVDLGFDNLPP
                     AGTKFTLLVMDDRTGRLVHHGQVTPVADSKTLRFYADGLDVDAWTPTNPHLYRVALTI
                     EEPGGVSQREEKRIGFRFFESKNGHLYLNGKPIFLRGIAINPPGRGIPDNIEKSRRFA
                     EQYVDFMKSIHVNIIRIPDNDTWYDVCDEKGMMVFGGNYSGTVDGGKPPKDYDKAVDW
                     YETKAFAMIASHPSLMVYAMTNETPFAGRAAGEWEKFLSYAAVRLKKWDSTRVFIANA
                     GYGYGKAGDICDLHRYWGWYYSSPFTFLHARNDEDIIPFPKKAGQPITFTECVGNYSG
                     PDGRYNLTPNHKNPGSQLNWTGHASNDVQALLANEHQCFTFKQAAELNRRLRSVNPEL
                     SGVFPFTILFYNWNTVRDFIDMGPKPVTAQAKLSYSPVLVSWENWTTQVYAGTTLKPT
                     LHIVNDADDFSDLTAATFTYRIIDEAENTIVTESFSLPDIAYYSEFERTLSIPLPADL
                     AAGHYHLLGQVVKDGRLISENKDRIFIAGKDFPLSATRPQRQILPYDKITSFKNISPS
                     SVVLIGENGADQALVRNAADIKRFIAAGGRMLCLRQDSSHLPYLNAILAAPIRNVTMD
                     LDYSQYPPPPRPSRNGYYVNPERPGNPVFDGIDREELKVWSDYTGWKESDR"
BASE COUNT          863 a         1074 c          966 g          742 t
ORIGIN      
        1 agaataccga agtggattcg cccctaccct atgccgatta ttattacgtg gaagccttat
       61 tgagaatgaa aaaccgatcc aaatgaagaa gagcctgctg atagctgccg ggttgttgag
      121 cgcggggctg gcgaacgccc aaagggtcat ccgggccgac taccgggatg aggccggtag
      181 cttcagccgt agctatgcca cctgtgtcgg agcgggtcgc gcagccgaag gcttgcgcgc
      241 ggactggcag caacagctgg cgatggcggt aaaagccatc gggttccggt acatccggtt
      301 tcatggcctg ctatgcgacg agatgcatgt gtatacggaa gacaagacag gccggcctgt
      361 atataacttt cagtatgtcg acaagctcta tgactatctg ctgagcatcg gcatccgccc
      421 cttcgtggag ctgggcttca tgccccccga tctcgccagc ggcacaaaga ccgtattctg
      481 gtggaaaggc aatgtcacgc caccaaagga ttatcaaaaa tgggcggacc tcatccaggc
      541 cctcgtccgg cactggcagg agcgatatgg ccgcgaagag gttgagaaat ggtatttcga
      601 agtttggaac gagcccgatc ttcacggctt cttcaccggg acacaggacg attatttcaa
      661 actctataag acgacggctg aggcgatcaa atcggtctcc cccacctacc gtgtcggcgg
      721 accggccaca tcgggcagca agtggatccg cgagatgctg cagtactgcc gcaacaacca
      781 ggtgccgctg gatttcatct ccacccacag ctataacacg aaaaccgtat tcgacgaatt
      841 cggcgtccgc aaacgaacga tgctgccctc cggctacctg tggggcaatg tggtaaatac
      901 ccgacggatc atggacagct gcgcctacag ccatctcccg ttgcactata cggagatcaa
      961 ttcatctcct tcctcacgcg acccgctgca cgacagctat caaaacgcgg cttttctcct
     1021 caatacgctc aaacataccg aacacgccgc ggcttcgatg tcttactgga ccttcaccga
     1081 cgtcttcgag gaagccggtc cgggcccgac gcccttccat ggcggtttcg ggctgatcaa
     1141 tctccagggg ctgcgaaagc ccaccttttt cgcttatcaa ttcctgcacc ggatgggtaa
     1201 tacggcgctg cgggacacag attcgtgctc ctgggtttgc agaagcgata agggtgtgca
     1261 gacactattc tggaacctcc atctgccttt cgccgatgct ccctatttca atgacagcct
     1321 cttccggctg aataccccgc cggccccggc gggaacggtg acagtgaaac tagaccacat
     1381 ccccaatggc cgctaccgcg tcctggtcta tcatataggc tatcatcata acgatgtctt
     1441 cagcgcctgg ctcgagatgg gctcaccatc cgacctgtcc ggacaacagc aacagcggct
     1501 cgacgcgata gccaacggcg ctcccgaaag cagcgggacc gtgaccatcg atgacgggac
     1561 attcagccgg cgtttcgaca cgaaggagaa cgatatttac ctcatccaac tggaacgaat
     1621 atgaaaagat tatcactgct ggtattgctg cccttgttgc tcggggtaaa agctgaagat
     1681 gtcccccgga tgatcgtctt tacgaccctt cattcagtac gggtggacct gggtttcgac
     1741 aacctgccgc cggcgggcac gaaatttacc ctgctcgtca tggacgaccg gacaggcagg
     1801 cttgtgcacc atggacaggt tactcctgtt gcggatagta agacacttcg gttctatgca
     1861 gacggtctcg acgtcgacgc ctggacgccg acgaacccgc atctctatcg cgtggctctc
     1921 acgatcgaag aaccgggcgg agtatcgcaa cgcgaagaaa aacgcatcgg attccgcttc
     1981 ttcgagtcga aaaacggtca tctgtacctg aacgggaaac cgatctttct gcgcggcatc
     2041 gccatcaacc cgcccggtcg cggaatcccc gacaacatcg agaaaagccg ccgctttgcg
     2101 gaacaatacg tggactttat gaagagcatc catgtgaaca tcatccgcat ccccgataac
     2161 gatacctggt acgatgtctg tgacgagaag ggtatgatgg tcttcggcgg caattacagc
     2221 ggcacggtcg acggcgggaa gccaccaaaa gactatgata aggccgtgga ctggtatgaa
     2281 acaaaggcat tcgccatgat cgccagccat ccaagcctga tggtctatgc aatgacgaac
     2341 gagacgccgt ttgcggggcg ggccgcgggt gaatgggaaa agtttctctc ttatgcggca
     2401 gtccggctga aaaaatggga cagtacacgc gtattcatcg ccaatgcggg ttatggatac
     2461 ggaaaggcag gggacatctg cgatctgcat cgttactggg gctggtacta ttcttcgccc
     2521 ttcaccttcc ttcacgcccg gaatgatgaa gatataattc ccttccccaa aaaagccggg
     2581 cagccgatca cgttcaccga atgcgtgggc aactattccg gaccggatgg aagatataac
     2641 ctgactccca accacaagaa cccgggctca cagctcaatt ggaccggtca cgcgtccaac
     2701 gatgtccagg cgctgctggc aaacgaacac cagtgtttta ccttcaaaca ggccgccgag
     2761 ctcaaccgcc ggttgcgcag cgtcaatccg gagctgagcg gcgtcttccc cttcaccatc
     2821 ctgttctaca actggaatac cgtgcgggat ttcatcgaca tgggaccgaa acccgtcacg
     2881 gcgcaggcga agctctccta ctcaccggta ctggtcagct gggagaactg gacaacacag
     2941 gtctatgccg gcacaactct taaaccgaca ctgcatatcg tcaacgacgc agacgacttt
     3001 tccgatctga ccgccgccac atttacatac cggatcatcg acgaggcgga aaacaccatt
     3061 gtgacggaaa gcttttccct gcccgatatc gcctactatt cggaattcga acgaacgcta
     3121 agcattcccc tgccggcgga ccttgccgca gggcattacc atctgctcgg tcaggtggtc
     3181 aaagacggca ggctgatctc ggagaataag gaccggattt ttattgcggg taaagatttt
     3241 ccgttgtcgg ccacaagacc acagcggcag atcctgccct atgataagat cacttcgttc
     3301 aaaaatattt caccctcctc cgtagtcctt atcggtgaga atggcgccga ccaagcgctg
     3361 gtcaggaatg cggccgacat caaacggttc atcgccgcag gcggccgaat gctctgtctc
     3421 cggcaggaca gctcgcatct gccatatctc aatgccatac tggccgcccc catccgcaat
     3481 gtgacgatgg acctcgacta ttcccagtat ccgccaccgc cacggccctc gcgcaacggt
     3541 tactatgtca atcccgaacg gcctgggaat cccgtcttcg acggtatcga tcgcgaagag
     3601 ctgaaggtgt ggtcggacta tacaggctgg aaggaatcgg acaga
//
LOCUS       DASYSV010000176         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2209489, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000176 DASYSV010000000
VERSION     DASYSV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2209489"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2622)
                     /locus_tag="VGQ51_05335"
     CDS             complement(<1..2622)
                     /locus_tag="VGQ51_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9036017.1"
                     /translation="MTAKTCILALLALIASPALPGQSYYFRHLQVENGLSNNAVICSL
                     QDNRGFLWFGTKDGLDRFDGYSFKIFRNDPDDTTSIGSNFIHALYQDPDGTIWVGTEK
                     GLYSFDASTERFSLLPGLNPGPIRDVRMDGRHNLWFISGFSLFKKSPSATRPEYFPID
                     SFFEATSLCVTADGTLWATSSNGYVYRYEERSRRFQAFDCLAGTKSDRWIERAYPTSD
                     SAILIGTAVHGALLFDTRTLQCKAILTYNPDKTAIFVRNFVETGSNEYWIATESGIYI
                     YNRTTGRITNLRKQFNDPWSISDNAVYTFCKDREGGIWAGTYFGGINYYPGQYNTFKK
                     YFPKSGENSLGGYVVREIHQDSHGDLFIGTEDAGLDKLDPATGHFTHFQPTGARGSIS
                     YSNIHGLLVHGNELWIGTFEHGLDIMDIRTGKIIRHYGVGSGDHQLKSSFIYCITRTR
                     DDHILLGTTRGAYVYDQQHDGFTPLPGMPLNNWYSSLLEDDSGAIWAGTFGNGVNYYD
                     PRTGISNNFRYDGRLHNSLASDRVNSIYRDSKGRLWFATEGGLCRWNPATNPSSTSTI
                     PSAPSTGSFTRYTTRDGLPTDFILALLEDGNGNLWVSTSKGLVSFNPDSLYSTADHPS
                     LATGAPNARQLTVFTRENGLLNDQFNFSSAFRDSTGRMYFGSVKGMISFNPREFRKNT
                     VVPGVYITGFQVDNRELPIGRNGSPLQKAISFTDRVTLDHSQSTFSIDFAALGFTAPE
                     TTEYAYEMEGLDKDWIFLKKNRKAYFTGLAPGHYTFKVKASNSSGIWVDHETRLGIDV
                     LPPWWASTVAYGCYILAGLLLAWYFIRSYHRRVEEKNRRKIEQLETAKEKEILNAKIE
                     FFTNVAHEIKTPLTLIKG"
     gene            2841..>3780
                     /locus_tag="VGQ51_05340"
     CDS             2841..>3780
                     /locus_tag="VGQ51_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEV9036018.1"
                     /translation="MKKWKVLFLLLLISCAGFAQETIKGSVTDKDGKPVVAATITLKG
                     ANVSTTSDESGNFTIRARSGQTLIITSIGFEDHELKIGAGGAPILVRLATRSNSLDDV
                     VVVGYGTVKKKDLTGSVSVVNVADAEKNPTHDVAKMLQGQVAGVSVQGSGEPGGYVSI
                     KIRGVSSFDPNGNNANSPLFVVDGVILDAPFDFSVDDIESVQVLKDASATAIYGTRGA
                     PGVIIITTKRGRVGAPRVTWNSYVGLQNVPKKIPVLDRVGYQKSVNAADVNAGLTLAP
                     ANDPSSPSYVSTVNTDWQHAALKTGLIQDHNLGVSGG"
BASE COUNT          796 a          885 c         1235 g          864 t
ORIGIN      
        1 tcccttgatg agcgtcaggg gcgttttgat ctcgtgtgcg acattggtaa agaattctat
       61 cttggcattg aggatctctt tttccttggc cgtctcgagc tgttcgatct ttcttctatt
      121 cttttcctct accctgcggt ggtagctgcg gataaaatac caggccagga ggaggcctgc
      181 caggatatag cagccataag cgaccgtgct cgcccaccac ggcgggagga catcgatccc
      241 gaggcgggtt tcgtgatcga cccagatgcc gctgctgttg gaggctttca ccttgaacgt
      301 atagtgtccc ggcgccagac cggtgaagta agccttgcgg ttctttttga ggaagatcca
      361 gtctttgtcg aggccttcca tttcataggc gtattcggtc gtctcgggcg ccgtgaagcc
      421 cagtgcggcg aaatcgatgc tgaacgtgga ttggctgtga tcgagagtga ccctgtcggt
      481 gaagctgata gctttttgaa gcggggagcc gttgcgtccg atggggagtt cgcggttatc
      541 gacctggaag ccggttatat aaacgcctgg gaccaccgta tttttacgga actcacgggg
      601 gttaaagctg atcatgcctt tcacgctgcc gaaatacatc cggccggtgg agtcgcgaaa
      661 ggccgagctg aaattgaatt gatcgttgag gaggccattc tcgcgtgtga agactgtcaa
      721 ttgccgggcg ttgggggcgc cggtcgcgag ggaagggtgg tcggcggtgg aataaaggga
      781 atcggggttg aaggagacga ggcctttgga ggtgctgacc cagagattgc cgttgccgtc
      841 ttccagcagg gcgaggatga aatcggtggg taagccgtcg cgggtggtgt agcgggtgaa
      901 ggacccggtc gatggagcag agggtatggt ggaggtggag gatggattgg tggccgggtt
      961 ccaacggcag agaccgccct cggtggcaaa ccagagtcgg cccttactgt cgcggtagat
     1021 gctattgacg cggtcgctgg cgaggctgtt gtgcagcctg ccgtcatagc ggaaattatt
     1081 cgagatgccg gttcggggat cataatagtt gacgccattt ccgaaagtgc cggcccagat
     1141 ggccccgctg tcgtcttcca gcagcgagga ataccaattg ttgagcggca tgcccggcag
     1201 gggggtgaag ccgtcgtgtt gctggtcgta gacatatgcc ccgcgggtcg tgccgagcag
     1261 gatatgatcg tcgcgggtgc gtgtgataca atagatgaag ctgctcttga gctggtggtc
     1321 accgctgcct acgccgtagt gccggatgat ctttccggtg cggatatcca tgatatcgag
     1381 accgtgttcg aaggtgccga tccacagctc gttgccatgg accaggagac cgtgtatatt
     1441 ggaataggag atgctgccgc gtgccccggt cggctggaag tgcgtgaaat ggccggtggc
     1501 gggatcgagc ttgtcgagtc ctgcgtcttc ggtgccgatg aacagatcgc cgtggctgtc
     1561 ctggtggatc tcacggacca cgtagcctcc cagggaattc tcgccacttt tggggaagta
     1621 ttttttgaat gtattgtatt gtccggggta ataattgatc ccgccgaaat aggtgccggc
     1681 ccagatgccg ccttcgcggt ctttgcaaaa ggtgtagacg gcgttgtcgg aaatggacca
     1741 ggggtcattg aactgtttgc ggagattggt gatcctgccg gtggtgcggt tgtagatata
     1801 gatgcccgac tcggtggcga tccaatattc gttggaccct gtttctacga aattccggac
     1861 gaagatggcc gtcttgtcgg gattataggt taggatggcc ttgcattgga gggtacgggt
     1921 atcgaagagg agggcgccat gaacggctgt cccgatgagt atggcgctgt cggaagtggg
     1981 ataggcgcgt tcgatccacc ggtcggactt tgtgccggcg agacaatcga aggcctggaa
     2041 ccggcgtgat cgttcttcat accggtagac atagccattg ctggacgtgg cccagagcgt
     2101 gccgtccgcc gtcacgcaga gtgaggttgc ctcgaagaaa ctgtcgatgg gaaaatattc
     2161 cggccgggtg gctgagggag actttttgaa cagggagaac ccggagataa accagagatt
     2221 gtgccggccg tccatgcgaa cgtcgcgaat gggccctgga ttaagtccgg gaagaaggct
     2281 gaagcgttcg gtggaagcgt cgaagctgta aaggcctttt tccgtgccga cccagatggt
     2341 gccgtcaggg tcctggtaca gcgcatggat gaaattgctg ccgatgctcg tagtgtcgtc
     2401 gggatcgttg cggaagatct tgaaggagta accgtcgaag cggtcgagtc cgtccttggt
     2461 gccgaaccat aggaatcccc tgttatcctg caggctgcag atgactgcat tgttggagag
     2521 gccgttctcc acctgcaggt ggcggaaata ataggactgc cccgggaggg cagggctcgc
     2581 aatcagggcg aggagggcca atatgcaggt tttggcggtc aataccgcta atttgagatt
     2641 ttttgtcgaa acaatgatac gaaaagatga aggcaatttt gggagaaggg tatttttgaa
     2701 ggtcgattaa atgtttaatt gacatttgtg agcccggcgg atgaccggaa tggatgcccg
     2761 ttggtcgggc ggcgggtttt aggggcggat ccggaaggtc gggcgacaat tgagattgtt
     2821 aattaccaaa atgacgctat atgaaaaaat ggaaagtcct tttcctccta ctgctgattt
     2881 cctgcgccgg gtttgctcag gagacgataa agggcagtgt cacggacaaa gacggaaagc
     2941 cggtggttgc agccactatc accctgaaag gtgccaatgt aagcaccacc agcgacgaga
     3001 gcggcaactt cactatccgg gcgcgttcgg gccaaaccct gatcattact tctattggat
     3061 ttgaagatca tgaattgaag attggggcgg gcggcgcccc gatcctcgtc cggctggcta
     3121 cccgcagcaa ttccctcgac gatgtggtag tggtcggtta cggcacggtg aaaaagaagg
     3181 atctgaccgg atcggtctcg gtagtgaatg tcgcggatgc cgaaaagaac ccgactcacg
     3241 acgtcgcaaa gatgttgcag ggccaggttg caggcgtaag cgtgcagggt tccggcgaac
     3301 ccggaggtta tgtgtcgatc aagatccggg gcgtcagttc gttcgacccc aacggcaata
     3361 atgcaaacag cccgctgttc gtagtggatg gggtgatcct cgatgcgcca ttcgactttt
     3421 ccgtggacga tatcgaatcc gtccaggtgt tgaaagatgc gtccgcaacg gccatctatg
     3481 gcacccgtgg cgctccgggg gttatcatca tcaccactaa aagagggaga gtgggtgcgc
     3541 cgagggtgac gtggaatagc tatgtcggtc ttcagaacgt gcctaaaaag atcccggtat
     3601 tagacagggt cgggtatcaa aaatcggtca acgccgcgga tgtgaatgcg ggtctgacct
     3661 tagcgcccgc caatgatcct tccagcccga gttatgtttc gaccgtcaac accgattggc
     3721 agcatgcagc attgaaaacg ggccttatcc aggatcataa cctcggagtt tcgggaggct
//
LOCUS       DASYSV010000177         5758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_288884, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000177 DASYSV010000000
VERSION     DASYSV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288884"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VGQ51_05345"
     CDS             <1..489
                     /locus_tag="VGQ51_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036019.1"
                     /translation="GPDQFLQVIHVIIATGEAVILQHPLAVMIGKRLPPLVEHRFITV
                     ENWSGLEGMGNFDVVRRQMPDGKVYEGRTVRTFDPKTRLWRLYWMDSNGGPIDPPVIG
                     SFENGVGLFFCKDYQVGRPVIIVFRWDKTNPNQPVWGQAFSDDNGKTWEWNSSNTSYR
                     VK"
     gene            544..1347
                     /locus_tag="VGQ51_05350"
     CDS             544..1347
                     /locus_tag="VGQ51_05350"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV9036020.1"
                     /translation="MKHRVCLLIALFSIGYTLRAQEKVLRKPSYIIIADGKLISRQQF
                     DVYAKSGDVKSMAKGVSQAERDRLAQQFGDTIGAKEFIVIITLLSDKAKAERIAAAKL
                     AKPDSAGPATNQSKATNESPLIGKAAANFHVRMSDGRDLQLFSLAGKVVMVNFWATWC
                     GPCLMEFYDFPEKIITPFADKPFVLLPIAKAESKETVLAKMRQLAKDGLHFPVGWDTD
                     NAIWEQLGGSSIPRTILIDKKGIIRWVSVGNNESNMAGLVEEIGRLVKE"
     gene            1532..2041
                     /locus_tag="VGQ51_05355"
     CDS             1532..2041
                     /locus_tag="VGQ51_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036021.1"
                     /translation="MNTRVQYDYNTLCILDYSDKELEESENPFAWVVLTAKKALLRGK
                     NLDKQLLKGKLWVFRKLMENGLFADAKLLAILKFLDKYIVFEETETNLIFNNRVDKIS
                     GKKNTMDILSEIVAEPLVRKKVRATVREIVRNLLTETSLSVEEIARSTNQTVSFVKSI
                     KNRLRKNIK"
     gene            complement(2472..2996)
                     /locus_tag="VGQ51_05360"
     CDS             complement(2472..2996)
                     /locus_tag="VGQ51_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9036022.1"
                     /translation="MQPTSFIITDFQPAHQPAFQHLYQEWFTSALHQPTEPADDFVLT
                     QPEKAILQKGGAILVASENGEIAGFVALKKTGASEMELTKMIIRPESRGKGLGEILCR
                     GILARGATLGAASVILYSHSSLQAALALYRKLGFQDVRLEQCLYSSFRCDIKMKKWLI
                     PAGSGKPEHSTSER"
     gene            3160..4155
                     /locus_tag="VGQ51_05365"
     CDS             3160..4155
                     /locus_tag="VGQ51_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036023.1"
                     /translation="MKKQFVLFACMLAAGSLSLFGQSSRSSADPELVLFGRGTYPGDD
                     SSFAMIRNSGFTTVMLSSFYIKANGDVYSGDDGHQPIVHEGRFTGSREWIKRIASLRS
                     GGSVRRIEILLEGRWFNQAPNTYDFIRDWIDSSKGAPGTVPGTGKSSTLFGILRSMKE
                     EIGVDAICIDDESVYDTASVIRLGEMANSLHLHMSLCPYARTHFWKAVLDGSQPGVVD
                     AIYLQCYDGGKNAVPGDWFRALGSAMPVYPIFLCRGAFSTCGVSHNSKSPEEIKSQLQ
                     EFKKGYPDMHGAGVWQMADIKSYVRQNCAATDPTSGTATSVPEYLSQLKTCLSVY"
     gene            4366..4962
                     /locus_tag="VGQ51_05370"
     CDS             4366..4962
                     /locus_tag="VGQ51_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV9036024.1"
                     /translation="MRKIIVTTNVTLDGVIQSPSGPGEDASGEFKYGGWSVPYADENS
                     GKAVQRYMKEKAEYLLGRKTFEIFNSFWPKHADIWPGVNEDTKYVLSRTMKNSDWKNT
                     VFLENVEDIRKLKATEGPDIQIWGSSELIQLLLKNGLIDELHLRIFPVILGEGKKLFK
                     DGQIATGFTLTENSITPKGVIIAFYKKTGEVKTGKLPV"
     gene            complement(5068..5499)
                     /locus_tag="VGQ51_05375"
     CDS             complement(5068..5499)
                     /locus_tag="VGQ51_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9036025.1"
                     /translation="MRIAESYLLIADDDPEDRQALSEAFADQHPSVTVKHVAGGHELL
                     RYLQECPIHQLPAVLVLDYQMPDLNGPQVLQYLTSHDRYRPIVIVMWSTSRRTKDIDE
                     CNRLGATHYLVKPDTIEELKNFIQKLNAIFETAARKARSLG"
BASE COUNT         1456 a         1270 c         1554 g         1478 t
ORIGIN      
        1 ggccccgatc aattccttca ggttatccat gtgataattg caacgggtga ggcggtcatt
       61 ttgcagcatc cgctggcggt gatgattggt aagcgacttc cgccgctggt cgagcaccgt
      121 ttcattaccg tggaaaactg gtcgggcctg gagggcatgg gtaacttcga tgttgtccgt
      181 cggcagatgc ccgatggaaa agtctatgaa ggccgcacgg tccgcacctt cgaccctaaa
      241 acacgtttat ggagattata ctggatggat agtaatggcg gtccgataga ccctcccgtc
      301 atcggctcct tcgaaaatgg tgtaggtctg tttttctgta aagactacca ggtaggccgc
      361 cccgttatca tcgtattcag atgggacaag acaaacccca accaacccgt ctggggacag
      421 gccttttccg atgataatgg aaagacatgg gaatggaact cttcgaatac ctcctaccgg
      481 gtgaaataaa cggagcgtac aggatttgat ccatcggatc gctttttttg ggtaaacttg
      541 cgtatgaaac atcgagtttg cctcttgatt gccctgtttt ccattggata tacgcttcgg
      601 gcacaggaga aggttttaag gaaaccctca tatattatca ttgccgacgg caaactcatc
      661 agcaggcaac aattcgacgt atacgcgaag agcggagatg tcaaatcgat ggccaaaggc
      721 gtgagtcagg cagagcgcga cagactcgcg caacaattcg gtgatactat tggtgcaaag
      781 gagtttattg tcatcataac attgcttagc gataaggcga aggcggagag gatcgctgcg
      841 gctaaacttg ccaaaccgga tagcgccggg ccggccacaa accaaagcaa agccacaaat
      901 gaaagccctc tgatagggaa ggctgcggct aactttcatg tcaggatgag tgatggacga
      961 gacctgcagc ttttttcgct tgccggaaaa gtggtgatgg ttaatttctg ggcgacatgg
     1021 tgcggcccat gtcttatgga gttttacgat tttcccgaaa agatcataac gccattcgcg
     1081 gacaaaccat tcgtcttatt gccgattgca aaggcggagt cgaaggagac cgtgcttgca
     1141 aaaatgcggc aactcgccaa agacggatta cattttccgg tcgggtggga caccgacaat
     1201 gcgatctggg aacagttggg tggcagcagt atcccccgaa cgatcctcat cgataaaaag
     1261 ggtattatcc ggtgggtttc cgtcggaaat aatgagagca atatggcagg gcttgtagag
     1321 gagatcggtc ggttggtgaa agaataggat aatcaatggc tatgaaccgg cacttcactg
     1381 atctcggggt ccgccgttcc tgcattcttc ttttcgtggc tgggtacaat tatttgcttt
     1441 cgctttcctc gtgacgtatc tttttattgc agctgctact tccccaattt aaactacgcg
     1501 atacaagtct tacttttata gacacgaatt catgaacacc cgggtgcagt atgattataa
     1561 tacgctttgc attctggact attcggacaa ggaactggaa gaaagtgaaa atccatttgc
     1621 ctgggtggtt ttaaccgcca aaaaggcgct gttaagagga aagaatctcg acaaacaatt
     1681 gcttaaaggt aagctgtggg tttttcgaaa attaatggaa aacggccttt tcgcggacgc
     1741 caaattatta gcgattttaa agttcctcga taagtatatc gtttttgaag aaaccgaaac
     1801 aaatcttatc tttaacaata gagttgacaa aatctctggt aaaaagaata cgatggatat
     1861 cctttctgaa atagtggctg agccattggt gcggaaaaag gtgcgggcaa cagtacggga
     1921 aattgttagg aatctcctta ctgaaacgtc cttgtctgtt gaagagattg ccagatcgac
     1981 caaccaaact gtaagcttcg tcaagagcat aaaaaatcgt ctgaggaaga atataaagta
     2041 aactcttgct cctacgtagc agtaataaat aatactctcc agtggcctat tcgggccatc
     2101 gttccgatct tacaacgcac ttcaatattg gaatacctcc tttgcgctac tttttttgat
     2161 cgcggcaatg taaaagttca tcttctgggg ctatcgggct cattgagcgg tggccccgga
     2221 gattgcggaa gatcggggtc cgtttccggg ctgatggccg ggctttccgg tcgtcccggc
     2281 gatagcggtt gccatttgtc gggcttgcca gggatggctg ccggcgcctt ttgcggtgga
     2341 aggccggctg gaggaacatc ctttgccggc ttgccggaag gctcgtagga ttgggcgtta
     2401 tttccaattg tttgagacat atcgggctct aatgaatcga tgcaatcccg atgccacgcc
     2461 taaggctgga tctatctttc cgacgtagaa tgctcgggct tacctgatcc ggccgggata
     2521 agccatttct tcatttttat atcgcagcga aagctagaat acaggcactg ttcgagccgg
     2581 acgtcctgaa aacccaattt gcggtaaagc gccagggcgg cctgaagtga tgagtgcgaa
     2641 tagaggatga cactggcagc cccgagcgtt gctcctcttg cgaggatgcc gcggcatagg
     2701 atctcgccga ggcctttgcc ccgcgattcc ggccggatga tcatcttggt aagttccatt
     2761 tccgaggcgc ccgtcttttt caacgctaca aagccggcaa tctcgccgtt ttcagaggcc
     2821 accaggatgg caccgccctt ttgcagaatg gctttttcgg gttgtgtcag tacaaaatca
     2881 tcggctggct cggtgggctg gtgcaaggcg gaagtaaacc actcctggta aagatgctgg
     2941 aatgccggtt ggtgcgcggg ttggaaatcg gtgatgataa agcttgtggg ttgcatacgg
     3001 aggaattgac aaccgtaaag atggcattct cgcgacggat aaaacagatg cagttttgat
     3061 aattttgact ggatcagttt gtcacgcaaa ctatatacta aattcgcgct ggacaggggt
     3121 gaaaacaccc atactgtgat gggctctaat ttagtcatta tgaaaaagca atttgtatta
     3181 tttgcctgca tgctggccgc cggcagtctt tcacttttcg ggcaatcctc gcgatcatct
     3241 gctgacccgg aactggtctt atttggacgc ggcacctatc cgggtgatga tagctctttt
     3301 gccatgatcc gcaattcggg atttactacg gtcatgttat ccagtttcta tattaaagca
     3361 aacggcgatg tgtattcagg cgatgacggc caccagccga ttgtccacga agggcgcttt
     3421 accggcagcc gggagtggat caaaaggatc gcctctctgc gctccggtgg ttcggtcaga
     3481 cggatcgaga tcctgttaga aggccgctgg ttcaatcagg ctccaaatac atacgatttt
     3541 attcgcgact ggatcgacag cagcaagggg gcgccgggta ccgttccggg cacagggaaa
     3601 agcagtacgc ttttcgggat cttacggtca atgaaagagg agattggagt cgacgcaatt
     3661 tgtatcgatg acgagtcggt gtatgacacc gcctcggtca tacggctggg cgagatggcg
     3721 aacagcctcc acctgcatat gagcctgtgt ccctacgcaa ggacacattt ttggaaggcc
     3781 gttctcgacg gtagccagcc gggtgtcgtg gacgccattt atctccaatg ttatgatggc
     3841 ggcaagaatg cggtcccggg ggactggttt cgcgcgttgg ggagtgcgat gccggtgtat
     3901 ccgatttttt tatgccgcgg cgcttttagt acatgcggtg tttctcacaa tagcaagtca
     3961 ccggaagaaa taaagtcgca gctgcaggag ttcaaaaaag gttacccgga tatgcacggt
     4021 gccggcgtgt ggcagatggc ggatatcaag agttatgtgc gtcaaaactg tgcggcgacc
     4081 gatccaacct cgggcacggc cacaagtgtg ccggaatatc tttcgcagtt gaagacgtgt
     4141 ttgtcggtat attgattctg tcaggcatcg tcactttcag tgtgggaggc atcgatgccg
     4201 catcggtcag atcggccggc tgtgcgagtg tctgtcggct atttgccggg atcttccggc
     4261 ggagggtgtc gcaatccccc cataagttgt cgtgttcata cagcatatat ttctgaaaag
     4321 cattcgatat ttgtgtcgtt agtaaacata aaaaaatcaa acaacatgag aaagatcatc
     4381 gttaccacaa atgttaccct ggacggagtt atacaatcgc caagcggacc cggggaagat
     4441 gcatcggggg aatttaaata cggcggctgg agtgtgccat atgccgatga aaacagcggt
     4501 aaggcggtac aaagatacat gaaggaaaag gcggaatatc ttttaggcag aaagacgttc
     4561 gagattttca attccttttg gcccaaacat gcggatatct ggccgggtgt caatgaagat
     4621 accaaatacg tcttgtcccg gacaatgaaa aactccgatt ggaaaaacac ggtgttcctc
     4681 gagaacgtgg aagatatcag gaagttgaaa gctacggagg gacctgatat tcagatttgg
     4741 ggaagtagcg agcttattca attgctgttg aaaaatggat tgatcgacga gcttcacctt
     4801 aggatttttc ctgtgatcct gggcgaaggc aagaagttgt ttaaggatgg ccaaatcgca
     4861 accgggttta cgcttaccga aaatagtatt accccaaagg gagttattat tgccttttat
     4921 aagaaaaccg gcgaggtaaa gacgggaaag cttccggtat aggcttatcc tggttgaatt
     4981 gtttcatctc ggccgaccaa cccacccggg gcgtcgaata aggttaggat tcgcaagatt
     5041 cgttcggatc gggaatagct ggttaactca acctaaggat cttgctttcc gtgcggcagt
     5101 ttcgaagatg gcattaagct tttgaataaa gttctttaat tcctcgatgg tatcgggttt
     5161 gacaagataa tgtgttgcgc cgaggcgatt gcattcatca atatccttgg tgcgtctgga
     5221 ggtgctccac attacgataa cgattggcct gtagcggtca tgtgatgtga gatactggag
     5281 gacctggggt ccattgagat cgggcatctg atagtccagc accaggacag ccggcaactg
     5341 gtggatcggg cactcctgca ggtagcgcag gagctcgtgg ccgcccgcta catgcttaac
     5401 ggttaccgag ggatgttgat cagcaaatgc ttcgctcaag gcctggcggt cttccgggtc
     5461 gtcatctgcg atcaatagat atgattctgc aattcgcatg acatattgct ttagaataaa
     5521 gatactcttc ccgtttgtta acggtttttc tcttttgtta atggcgatag gaagatacag
     5581 gtttccgata ggtgttacgg tcaaccatct tttccggaag aggaaaagaa tttgtctatt
     5641 ataatttaag ataaggaaaa aaccatgccg aaattgtttt agaggtactc tgcgcttagt
     5701 atcaactaat taagcggcga taaccacatt acgattcgcg attaataaaa tgattggt
//
LOCUS       DASYSV010000178        15284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_805223, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000178 DASYSV010000000
VERSION     DASYSV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15284
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805223"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="VGQ51_05380"
     CDS             <1..1657
                     /locus_tag="VGQ51_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9036026.1"
                     /translation="SHGELSYEVLPGLELKTSLGYTDLQTGEYTDAPTAAIDPTLRSF
                     VPSSADFANYDYQTWIVEPQASYKVKLGGGKLSALVGGTIEQNTSQGQVLQATGFTSD
                     QLLGNIQAASSIVVNAVTNTQYKYGAIFGRLGYSWMDKYLLDLTARRDGSSRFGPGKQ
                     YANFGAAGGARIFSREEFVQKALPFLSFGKLRASYGTSGNDQIPDYGFMSLYSSTINP
                     FLGSQGIYPYNLANPDYAWEINKKLEGGIELGLFKDRILAGASFYRNRSSSQLVGYPL
                     STVTGFTSVQANLAAVVQNTGWEFSVTTKNIAGKDFSWTSNINLTIPRNKLLSFPGLA
                     SSSYAGSYVIGQPLTIFKAAHSLGADSATGSYVFSDVKGNPTFNPTFPDDYTVLVDLA
                     PKFYGGFENSFRYKGFQLDFLFQFVKQTGAIFISNELPGVEGIQQPVAVLKRWQKPGD
                     KTNIQKFNEDYSLDQAWYDYAENSDGGYGDASYIRLKNLSFSYTLPSSWEHKLHLQNC
                     RVYVQGQNLLTITHYAGMDPENPSVVPSLPPLKVITAGIQLGL"
     gene            1793..3148
                     /locus_tag="VGQ51_05385"
     CDS             1793..3148
                     /locus_tag="VGQ51_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9036027.1"
                     /translation="MQIQAPPTQIAASTAFADNATAASVLTGIYDQIQGNGAFAGGLQ
                     SLTLLGGLSADELQLYSSTGFSANDVGYYKNAVPNSSTLLWSEPYKYIYDANAAIEGL
                     KDSTAGVSASVRQQLTGEAKFIRAFFYFYLTNLFGDVPLATTTSYTVNNNLPRTPKAQ
                     VYQQIVADLKDAQNSLSPDFLSTDAVTVTVDRVRPTKWAATALLARVYLYTGDWASAQ
                     AQATTVINNTGSFSILANLNSVFLKNSREAIWELPPVIPGENTLDGQTFILTTAPNSI
                     RPVALSSELVNAFEPGDKRDTAWVGNITRVGKTYYYPHKYKAGFVPGSTPTEYLMMLR
                     LSEQYLIRAEAEAQQNDTADARKDVDVIRNRAGLPNSTVAADKDSQLAAILHERQVEF
                     FAEWGHRWLDLKRTGTIDAVMPAVDAIKGVTWASYKQLFPIPLSDMQKDPQLTPQNSG
                     Y"
     gene            3160..4392
                     /locus_tag="VGQ51_05390"
     CDS             3160..4392
                     /locus_tag="VGQ51_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV9036028.1"
                     /translation="MKRLFFYCLLALIPTCVVAQGIHFSDDLSWSQIVAKARAENKYI
                     FVDCYTTWCGPCRSMKENVFPLKDVGDFYNAHFISVSVQMDSTRKDNDTVRNWYAEAH
                     AMKTRFGVNVFPTYLYFSPDGQPVHEGNAAIPVARFIALGEDALNPAKQLFPLVSRFE
                     TGDRDTSLCKQIAELTYFSDHKIAGEAADAYIAQMPDQLTKANLMFICQYTFSVRSRA
                     FHVLLDNLQKAREVIGAYTVDMTLINAIVVDESDPFLGKSATSTDWKVLEGYLNKKYP
                     AIAAEAFGRAKDRFYYEGKDYRTYVDCFSDFYKKYPQLTPMMMLNTRAWDMFRKSSDK
                     HVLTVALGWSKACNDAENNATYKDTYANLCYKLGRKEEAMKWEQDAINMSSDADDKKP
                     FEVVLEKMKKGEATWPQS"
     gene            4446..7100
                     /locus_tag="VGQ51_05395"
     CDS             4446..7100
                     /locus_tag="VGQ51_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV9036029.1"
                     /translation="MKQCIIGMLLFCSAVSVNAQKKPLDESAIDNWPKIYAPAISPDG
                     KFVTYTLSEGEGSKLIIKATDGSWERDVPGGMNPTITEDGRMVVISLPHDSLEIVRPG
                     SKSKIEGIAGVSAYKTPKEGNGQWLAYRTTDKRLVLRDMFSGSEKSFSSVDDYAFSDL
                     GNVLVVSEANGLLWIDLPGGSVDTICRGSGKVGYFGFDVAGNQLACIVSRGKDHSLVY
                     YSRGMDSAVVRVDDRTPGMEKGVVIAGRPPSFSPNGNRIFFSLMPDRSAAGQGGSKGV
                     QVDVWGYEDEVLQSKQLVQMKYGQLFTAVLNKDQHAVIRINTNQDKPYVQLPDGGNGD
                     WAMVETIEDKSEEYWRPSARPSLYLVSLDDGTRKLVKKELLAPKSLMSLSPQGGYVLW
                     YDMGRACYYTYCVADGKIQNISGRIPVPLFDEGSDDLPQHMPIGIAGWLDNDQAVLLY
                     DKYDIWKVDPAGTKPPENLTHGYGREHGVILRYLSLKFDLIPAIPHDGQLLLTALDLS
                     NKDNGIFRQGLGVQEKPDRLTMGPSVYYYSFYPWPSTTANVPMKPVKAKKAEMYVVER
                     MSAKDFPNLYATSDFIHYSPMSNEQPQKLYNWLSTELVHWGLPDGRRGEGILYKPADF
                     DPKKKYPVIFHFYEKDANGLNFFIDPDWSHGPINIPWFVSHGYLVFDPNIHYTIGAPG
                     ESMVNSVVSAARYLATRPFVDAGKMGLQGHSFGGYEVDYLVASTNFFAAGISVSGISD
                     PVSFYGEIRPGFGETSMQLYETGQNRMGTTPWKNPAAYLESSPILKANKVRTPLLLVN
                     NVGDGNVPFMQGVEMFADLRRLGKPVWMLQYDGEGHTISNEENKRDFTIRMEQFFDHY
                     LKGIPAPEWMTKGVPARLKGIDDGLKMP"
     gene            complement(7418..7672)
                     /locus_tag="VGQ51_05400"
     CDS             complement(7418..7672)
                     /locus_tag="VGQ51_05400"
                     /inference="COORDINATES: protein motif:HMM:NF039804.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6520 family protein"
                     /protein_id="HEV9036030.1"
                     /translation="MKRSKLILNGAAVVVALTGAFAFTNHFSDANLWTLQPNQHCTQI
                     QCASTDVEGTLTPCPDLNAKFLNNKCTIPFTGTGFFVEQQ"
     gene            complement(7778..8221)
                     /locus_tag="VGQ51_05405"
     CDS             complement(7778..8221)
                     /locus_tag="VGQ51_05405"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEV9036031.1"
                     /translation="MKKQKMIDIIAFLLIVLFVYEGTNNLLRHAWFALTLESLPHVGS
                     LGGLFSWAVPTAELTVALLLTFSRRRQRALLASLFMMLGFTVYSAWLFWVAPEFPCNC
                     GGFIQEMTWGFLIPFNLAFAGLAFIGLRMDRQLRKSLRNTMPAIS"
     gene            complement(8232..10871)
                     /locus_tag="VGQ51_05410"
     CDS             complement(8232..10871)
                     /locus_tag="VGQ51_05410"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HEV9036032.1"
                     /translation="MITAAMEDYGNILGHHGLTFKKDNLFLTVGDPRPAPGWILHLSV
                     VASQFEKFLEAVLPLLIGENMPFKIARDKTTAQSILEGKLGYTRLGKVISIFPDDENR
                     ALSLARKLTTLTSTFKGPHILTDRHLGGRVYTRYGYFSAINGTPEIPYTIPFTLAPGL
                     QWPFSSLAQANPPAANKVLNNKYRTMATLKEDARGNVHKALLLGQGWKIKWCLLKEGN
                     PCMNADEQERDIVDRLIWQYQLQNELYESLPVPKAFELFKENDRTYLAMEYIRGTLLN
                     EKIASVFKGRPWRMPPQRSRLRLLGYALQLIDIAEKMHRKGFIHRDITVANFIVTRGG
                     RLTMIDLELVYSKTNRNPLPPFGSGTEGFMSPEQRESRTPTEKEDVYAVGASLIVLLT
                     NLLPGKFSTTNPQLLIEQLRFFLGDHALLPVLAACFNDDPALRPDLSELKTTVRQFVL
                     VPGGAGAPGAAGAPGAAGVPGRAANPIGHGEKKDLGETIDQALNALNTTIMKGSEDVW
                     WSRTIQNKAFYYYQSQSASIYPGFHTGIAGVLYLLARAYRSGYPIRSCGASYTDGWKI
                     IRTKFLQRLQSISGGLYHGTSGIAMALAEGIENGLIPDKQQALQDLGACLYNPRLKDL
                     SLASGFAGQGLALLRCAHLLDGQLVSERLRVMVDHLLRTQQPDGSWTDTDVATPGTAW
                     TGLADSTAGITCFLLSYLIWQKNPPLEAATLRALQWMTRRLTRDPRPLLERLIRPNGP
                     HPPAGLENGPAGTALIFIKAFELFGEPAYRQTAETLLRKYPPYIVQQNVSLGNGLAGL
                     GETCLEAARVFRSHEWSDRAEWIAQLLAHTGYRQPDDSCYWLTEADPLSHTADLFVGN
                     AGIIHFLIRVHSPGALSHPILTI"
     gene            complement(10868..11290)
                     /locus_tag="VGQ51_05415"
     CDS             complement(10868..11290)
                     /locus_tag="VGQ51_05415"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEV9036033.1"
                     /translation="MKRTTIYHFISWLFIALFLYTAVSKLLDWQAFLGQLEESPLIGP
                     NLARFAWVVPAVELSIAVMLGLPRLRIAGLYASMILMIGFTIYTIALTSSGPDLPCGC
                     GGFLEQLPPVTHIVLNVILSLLALAGARMITIHSKTSI"
     gene            complement(11287..13776)
                     /locus_tag="VGQ51_05420"
     CDS             complement(11287..13776)
                     /locus_tag="VGQ51_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HEV9036034.1"
                     /translation="MPYPMLYHITDNAGSPPLPAATNTGGSYPAILTEFGLSFKTDGY
                     YLLVGQMPQMTEWVLHVSVIAASLEELLKTILPLLAKENVCFRLAANKGMAQLILNGG
                     LGPTALGKVIKLYPVNNRQAASLAGQLIAATLPFKGPRIPTDRHLGGLLYVRHQPSPN
                     KLPFHLPADIHWPFDRFAPRATSAQETALPGKYKTFSVLKEDPRGDVKKALWLEKWYK
                     IKWCVIKQGRQSMAADDDGRDTSDRLRWQFRLHRDLQGSLPLPRAYDLFEAGGDTFLV
                     LEYIKGACLEDVILETYRSMPWWRLSQPHRLRLLEYALRLLDIIQRMHDKGYAHRDIT
                     PLNFLVARHGLVMIDLELAWSTSLHQPSPPFRYGTPGYMSPEQHNVQPPTSGEDIYAI
                     GATLVALLTGLIPNKFSTPYQHILQMQLRFFIPDPMLVSIISSCFLDSPARRPGLHLL
                     KKNIREFRDKQLPQCSVPLSATGKPDSVKIERAISSALQALTMPPLTSPAKCWPAGVL
                     YLLAQAQKAGFDIHSCMTEYANSLAYMHLQCEKPARLPAGWFEGAAGMALAMTEGLKN
                     NVIVTRLQSVGDIQSCLLNGTTEDWDLANGMTGRGMVSLNAADLLGGDWVWLLLHSNV
                     EQLISAQQRDGSWISGSHGKGHMKLPGFAHGTAGIICFLLAWSGRFQDCAAQKAAARA
                     LYWLGRRNYPSDPGLSEGLAGIILAFLKGYEKLGDPIYRLLAESALKALPDFYVDRDL
                     TLSSGLAGIGETWLEAARVLRSQEYREKAGWLVRQLQHQYRQQGDGSRYWVQDHTPYP
                     TPDLLTGNCGIIHFLLRYHRPDQLNHPLLPV"
     gene            complement(13792..14283)
                     /locus_tag="VGQ51_05425"
     CDS             complement(13792..14283)
                     /locus_tag="VGQ51_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036035.1"
                     /translation="MKKILLIGAIACFMACRHRSGVIPNITSLPAFTMLLIDSTTHLA
                     SAAIPSGAPLIFMYFRPDCRHCQADTRLLIKNMDSLKSTHIYLLTPMPFKELKGFYDS
                     FDLGRFKNITVAEDEGYSFYKSFQPAAVPYMAIYDSHKRLVNTYLGDRQIQMNNIFKA
                     IYQ"
     gene            14485..>15284
                     /locus_tag="VGQ51_05430"
     CDS             14485..>15284
                     /locus_tag="VGQ51_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEV9036036.1"
                     /translation="MKQLLFIVICLPLFVDAQPAAVDTHAWQPASLTLPTRWSKSVSP
                     SNALPEYPRPQLVRGEWQNLNGLWDYSITDSASAEPKSYAGQILVPYPLESALSGVKG
                     ALRPEQRLWYRRDLAIGSLRKERRYLLHFGAVDYQATVYVNGKAMGQHTGGYQEFTID
                     ISGALHKGTNRLTVAVLDPTDKGNNPHGKQTLDPKGIWYTPSSGIWQTVWLETVPTVY
                     IEGLELTPDVDNGVLKVKANVKAAGKGYTVEAVAFSGSAMTGSVKGAPG"
BASE COUNT         3599 a         3667 c         4462 g         3556 t
ORIGIN      
        1 cagccatggc gagctcagct atgaagtttt acccggtctg gagctaaaga ccagccttgg
       61 ttatacggat ctgcagacag gagagtatac cgatgcgcct acggctgcca ttgacccgac
      121 tctgcgttcg tttgttccaa gctccgccga tttcgcaaac tatgattacc aaacatggat
      181 cgtcgagccg caggctagtt ataaagtgaa gcttggcgga gggaagctca gtgcgcttgt
      241 cggcggcacc atcgagcaga atacgagcca gggtcaggtg ctacaggcga cgggctttac
      301 cagcgatcaa cttttgggaa atatacaggc ggcctccagt attgtggtga acgccgttac
      361 caatacccaa tacaaatacg gggcgatctt tggccggctc ggctatagct ggatggataa
      421 atacctgctg gatctgaccg cccgcaggga tggttccagc cgttttggcc ccgggaagca
      481 atatgcaaat tttggtgcgg caggcggcgc acggatattc tccagggagg aattcgttca
      541 aaaagcactc cctttcctga gttttggaaa gttgagagcc agctatggaa catcgggtaa
      601 cgaccagatc cctgactacg ggtttatgag cctttatagt tcgaccatca accccttcct
      661 cggttcgcag ggcatctatc cttataattt ggccaacccc gattatgcct gggagatcaa
      721 taaaaagttg gaaggcggga tcgagctggg tctctttaaa gaccggatcc tggccggcgc
      781 cagcttctat cgcaaccgaa gcagcagcca gctggtgggt tatcctctgt cgaccgtcac
      841 cgggtttacc tccgtgcagg cgaatcttgc ggcggtggta cagaatacgg gatgggaatt
      901 ttccgtaaca accaagaata tcgcaggtaa ggattttagc tggaccagca atatcaattt
      961 aaccattccc cggaacaagc tgttgtcttt cccggggttg gccagcagct cttatgcggg
     1021 cagctatgtt atcggacaac ccctgaccat ctttaaggca gcgcactcac tcggggcaga
     1081 cagtgcgacg ggctcgtatg ttttttcgga tgtcaaaggc aaccccacct tcaatccgac
     1141 ctttcccgat gactataccg ttctcgtcga tctcgcaccg aagttctatg gcggttttga
     1201 gaacagcttt cggtacaaag gctttcaact ggatttcctg ttccagtttg tcaaacagac
     1261 cggcgccatc ttcatttcca atgagctgcc aggagtggaa ggtatccagc agccagtcgc
     1321 cgtgcttaag cgctggcaaa aaccgggcga caagaccaat atccaaaagt tcaatgaaga
     1381 ttacagcctt gatcaggcct ggtacgatta cgctgaaaat agcgacggcg ggtatgggga
     1441 tgcctcttat atacggctca agaacctctc cttctcttat accttgccgt cctcatggga
     1501 gcataaactg catttgcaaa actgccgggt atatgtacag ggtcagaacc tgttgaccat
     1561 tacccattac gccggcatgg acccggagaa cccctcggtc gtccccagtc tgcctccttt
     1621 gaaagtgatc accgccggga tacagctcgg attgtaaaac agtggatgga catcaacgta
     1681 tcgattcatg aaaaaatctt ttgatcaaat gttacctgac tataaatttt caatacgccc
     1741 gctgattgtc gttggactgg ttgcgggcat gagtgtcgcg tgcaataaaa ttgtgcagat
     1801 ccaggccccg ccgacccaga ttgcggcctc tactgcattt gcggataacg caacggccgc
     1861 gtccgtgctg accggcatct atgaccaaat acaaggcaac ggagcatttg ccggagggtt
     1921 acaaagcctc accctattgg gaggtttatc ggccgatgag cttcagctct attcgagcac
     1981 cggcttcagt gcgaacgatg taggctatta taaaaatgcg gttcccaaca gcagcaccct
     2041 gctttggagt gagccgtaca agtacatcta tgacgcgaat gcagcgatcg aaggcctgaa
     2101 ggattcaacg gccggtgtaa gcgcgtccgt gcggcagcag ctgacgggcg aggcaaaatt
     2161 catccgcgcg tttttttatt tctacctgac caacctattc ggcgacgttc ccttggcaac
     2221 gactaccagc tatacggtga ataataacct tccgcgcact ccaaaggcgc aggtatatca
     2281 gcagatcgtt gccgatctga aggacgccca gaattcattg agcccggatt ttctttcgac
     2341 tgatgctgtg acagttactg tagaccgtgt gcggccgacc aaatgggcag ctaccgccct
     2401 actggccaga gtttatctct ataccggcga ttgggcgagt gcgcaggcac aggccactac
     2461 ggtgatcaat aacaccggca gcttttcgat cctggccaac cttaacagcg tcttcctgaa
     2521 gaacagcaga gaggctatct gggaattgcc cccggtcatt ccaggggaaa atacgctcga
     2581 tgggcaaact tttatactca ccactgcgcc taacagtatc cggccggtgg ctttgagcag
     2641 cgaactggtg aatgcattcg aaccgggaga taaacgtgat acggcgtggg tagggaatat
     2701 aaccagggtt gggaaaactt attactaccc gcacaagtat aaggcgggtt ttgtccccgg
     2761 ctcgactccc accgaatacc tgatgatgct gcgtctctcg gaacaatacc tgatccgggc
     2821 agaggcagag gcccagcaga acgatactgc cgatgcacga aaggacgtgg atgtgatccg
     2881 taacagggcc gggctgccga acagtacggt tgctgccgat aaggattcgc agctcgcggc
     2941 catccttcat gagcggcagg tagaattttt tgcagaatgg gggcatcggt ggctggatct
     3001 gaagcggacc gggacgatcg atgcggtcat gccggccgtc gacgcgatca agggggtcac
     3061 ctgggcttct tataagcagt tgttccctat cccgctgtcg gatatgcaga aggacccaca
     3121 gttgactcct caaaattcag gttattgaaa attattcgta tgaaaagact gtttttctat
     3181 tgccttttgg cattgatccc gacatgtgtc gtggcgcagg gtatccattt cagtgatgat
     3241 ctcagctggt cgcagatagt ggcgaaggct cgggcggaga acaagtatat cttcgtcgat
     3301 tgctatacca cgtggtgcgg tccttgccgg tccatgaagg aaaatgtatt tccccttaag
     3361 gatgtgggcg acttttataa cgctcatttt atcagcgtgt cggtgcagat ggattccacg
     3421 cggaaagaca atgataccgt gcggaactgg tatgcggagg cgcatgcaat gaagacgagg
     3481 ttcggggtca atgtcttccc cacctatctg tacttttcgc cggacgggca gcctgtgcat
     3541 gaagggaatg ccgccatccc cgttgcgaga ttcatcgctc tgggcgagga tgcgttgaac
     3601 cctgccaaac agcttttccc acttgtctcg cggttcgaga ccggcgacag ggatacttct
     3661 ctgtgtaaac agatcgccga attgacttat ttcagcgatc ataagattgc cggggaggcc
     3721 gctgacgcgt atatcgctca gatgcccgat cagctgacga aagcaaatct gatgttcatt
     3781 tgtcaataca cgttcagcgt caggagcagg gcattccatg tcctcctcga taatttgcag
     3841 aaggcgaggg aggtgatcgg cgcctacacg gtagacatga ccctgatcaa tgccattgtc
     3901 gttgatgaga gcgatccttt tcttggtaag agcgctactt ccaccgattg gaaagtgttg
     3961 gaaggctacc tgaataaaaa atatcccgcg atagcggcgg aggcattcgg gagggcgaag
     4021 gataggtttt attatgaagg aaaggattat aggacctatg tcgattgttt cagcgatttc
     4081 tataagaaat acccccagtt gactccgatg atgatgctga atacgcgggc ctgggatatg
     4141 ttcaggaagt catcggacaa acatgttctt accgtcgcat tgggatggag caaggcgtgt
     4201 aacgatgctg agaacaatgc tacgtacaag gatacttatg caaatctctg ctataaatta
     4261 ggcaggaaag aggaagcgat gaaatgggag caggatgcga tcaatatgtc aagcgatgcg
     4321 gatgataaaa agccgttcga ggtagtactg gaaaaaatga aaaaaggtga agcgacctgg
     4381 ccccagtcct gatgaaattt cgtaacttaa ggaggccgcg cgtcatcctt atcgcaagta
     4441 taccaatgaa gcaatgtatc atcggcatgc tgttattttg cagtgccgta tccgtgaatg
     4501 cgcagaagaa gcctctcgat gaatcagcga tcgataactg gccgaagatc tacgcgccgg
     4561 caatcagccc ggacgggaaa tttgtcacct ataccctcag tgaaggggag gggtcgaaac
     4621 tgatcatcaa ggccacggat ggttcgtggg aaagggacgt gccgggcgga atgaatccca
     4681 cgatcacgga agacgggcgg atggtcgtga ttagcctgcc gcatgatagc ctggagatcg
     4741 tacggccagg atctaagtca aaaatagaag gaattgctgg cgttagtgcg tataagacac
     4801 caaaggaagg aaatgggcaa tggctggcat accggactac cgataaacgg cttgtactgc
     4861 gggatatgtt cagtggatcg gaaaagagtt tttcttctgt ggatgattat gcgttcagcg
     4921 acctgggcaa cgtgctggtg gttagtgagg cgaacggttt gttatggatc gatctgccgg
     4981 gtggttccgt ggatacgatc tgtcggggta gtggtaaggt gggatacttt ggttttgatg
     5041 ttgcgggaaa tcagctggct tgcattgtta gcaggggcaa agaccattcg cttgtctatt
     5101 attccagggg aatggatagc gctgtggtcc gggtagatga tcggacgccc gggatggaga
     5161 agggggtggt gatcgcgggt cgtccgcctt cgttcagccc gaacgggaac cggatctttt
     5221 tttcgctgat gcctgacagg agcgctgcgg ggcaaggcgg gtccaaagga gtgcaggtag
     5281 atgtgtgggg gtatgaggat gaggttttgc agtcgaagca attggtgcag atgaaatacg
     5341 gccagttatt tactgcggta ttgaataagg atcaacatgc tgtaattcga attaatacga
     5401 accaggataa accatacgtc caactacctg atggcggcaa cggggattgg gcaatggtag
     5461 agactatcga ggataagtcg gaagaatatt ggaggccttc cgcccgaccc agcctctatc
     5521 ttgtctctct tgacgatggg acgcgcaaac tggtaaaaaa agaattgctg gcaccgaagt
     5581 ctttaatgtc tctatctccg caaggaggat atgtgctttg gtatgacatg gggagagcct
     5641 gttattatac ctattgcgtt gctgatggta aaatccaaaa tattagtggc agaatacccg
     5701 tgccgctatt cgacgaaggg agcgacgatc ttccgcagca tatgccaatc gggattgcgg
     5761 gatggttgga taacgatcag gctgttctgc tgtatgacaa gtacgatatc tggaaagtgg
     5821 atccggcagg gaccaagcct ccggaaaatc ttacccatgg atatgggcgt gaacacgggg
     5881 tcattttacg gtacctatct ctaaagttcg atttaatacc ggcgatcccg cacgatggac
     5941 aattgttgct aacggcgttg gatctttcga acaaagacaa tggtattttc agacaggggt
     6001 taggtgttca ggagaaacca gatagactca cgatgggtcc gtcggtttat tactattcat
     6061 tttacccctg gccgagcacc acagccaatg ttcccatgaa gccggtgaaa gcgaaaaaag
     6121 cggagatgta tgtcgtagag cgcatgagtg caaaggattt tccaaatcta tatgcaacgt
     6181 cggactttat acattattca ccgatgagca atgagcaacc gcaaaaattg tataattggt
     6241 tgagcactga gcttgttcac tggggtcttc ccgatggaag gaggggggaa gggatactat
     6301 ataagcctgc ggatttcgat ccgaaaaaga aataccctgt catttttcat ttttatgaaa
     6361 aggacgccaa tgggttgaac ttttttatcg atccggattg gagtcatgga cccataaaca
     6421 taccttggtt cgtcagccat ggatacctcg tctttgaccc taatatccat tatactatcg
     6481 gtgcgcccgg cgagagcatg gttaattccg ttgtatcggc cgcccggtac ctggcgacaa
     6541 ggccatttgt cgacgcaggg aagatgggtt tgcagggaca tagtttcgga ggatatgaag
     6601 ttgattacct ggtggcgagt acgaatttct ttgcagcggg tatatcggtc tcgggcatta
     6661 gtgatcctgt aagcttctat ggtgagattc ggccgggatt cggtgagact tccatgcaac
     6721 tttatgaaac cggtcagaat cgtatgggaa cgacaccctg gaagaatccg gcagcgtatc
     6781 tcgagagttc tcctatactg aaggcgaata aggtcaggac gccgctgctc cttgtgaaca
     6841 atgtagggga tgggaacgtg ccatttatgc aaggggttga aatgttcgcc gatctacgtc
     6901 ggttaggtaa gcctgtctgg atgctgcaat acgatgggga aggccatacg atcagcaatg
     6961 aagagaataa aagggatttt acgatccgta tggaacagtt cttcgaccat tacctgaaag
     7021 gaatccctgc gcccgagtgg atgacgaaag gggtaccggc gaggctgaag gggatcgacg
     7081 atggactaaa gatgccctag ttagcgatca atttaccagc tgtaacaaat tgggaggagc
     7141 cgagaatttc tctgcgcctc cccgagggtt gtctgagcgc caccaccgtt ggtccaaaga
     7201 tttgagttat tgtaatgaga cttgaatgcg aatgctgcgg tcaatgcgac tgcggcggct
     7261 ccattgagga tcaatctgct tcttttcata aaatagacgc ttttgttgcc caaaagctaa
     7321 agtttaagtg atataaaatt gttttaatta ggaagcgcgg agtttcctcc gcgcctcctc
     7381 atgaaacctt tcctaatgtt taatcaggac tcactggtta ctgctgctca acgaaaaatc
     7441 ctgtacctgt gaacgggata gtacacttgt tattcaggaa tttcgcattg agatccggac
     7501 aaggagtcag cgtgccttcc acatccgtac ttgcgcactg gatctgggta cagtgttgat
     7561 tgggctgaag cgtccaaaga ttcgcgtcgc tgaaatgatt cgtgaatgcg aatgctccgg
     7621 tgagcgcaac tacaacagct gctccgttga ggattaactt gcttcttttc ataaccaagg
     7681 tgctttttga tttttaaaaa gctaaacttt aattgatcaa atagtgccta ctctttttgc
     7741 aggttttcgg ctaaccctgc atgtctgcca agtctgatca ggatatggca ggcatggtat
     7801 tgcgcaaaga tttacgtagt tgtcgatcca tccggaggcc gatgaaggcc aggccggcga
     7861 aggccaggtt gaaagggatc agaaagcccc aggtcatttc ctggatgaac ccgccacagt
     7921 tgcatggaaa ttcgggggca acccagaaca gccatgccga atagacggtg aagcccagca
     7981 tcatgaaaag cgatgccagc agagcgcgct ggcgtcttcg ggagaaagtc agcaagaggg
     8041 caaccgtgag ctcggcggtc gggaccgccc aggagaagag gccgcccaac gaccccacat
     8101 gcggtaaaga ttccagagta agtgcaaacc acgcatggcg gaggaggttg ttggtgcctt
     8161 cgtatacgaa cagcacaatg agtaagaagg cgatgatatc gatcattttt tgttttttca
     8221 taatcggtgg tttaaatggt aaggatggga tggctgaggg cgcccggaga atgaacgcgt
     8281 atcaaaaagt gaatgatgcc cgcatttccg acaaagaggt ctgcggtatg agataaaggg
     8341 tctgcttccg tgagccagta acaggaatcg tcgggctgcc tgtacccggt atgtgccagc
     8401 aattgggcga tccattcggc gcggtcagac cactcgtggg atctgaagac acgtgcggct
     8461 tccaggcagg tctccccgag gccggcgagc ccgttgccaa gagaaacgtt ctgttgcacg
     8521 atatagggcg gatatttccg gaggagtgtt tccgcggttt ggcggtaggc gggctctccg
     8581 aataattcga atgcttttat aaagataagg gcagtgccgg cgggaccgtt ttcgaggcct
     8641 gcgggcggat ggggaccgtt gggccggatc agccgctcga gcaggggacg cgggtcgcgt
     8701 gtcagccgtc ttgtcatcca ctgcagggca cggagcgtcg ctgcctccag cggagggttc
     8761 ttttgccaga tgagatagct gagcaggaaa caggtgatgc cggcggtact atccgcaaga
     8821 ccggtccatg ctgtccccgg ggtggcaaca tcggtgtcgg tccaggagcc gtcgggttgt
     8881 tgcgtccgga gcaggtgatc gaccatgacg cgcaatcttt ccgataccag ttgtccgtcg
     8941 agcaggtggg cgcaccgcaa cagcgccagt ccttgtccgg cgaaaccgct ggcgagtgaa
     9001 agatctttca gccgcggatt ataaagacag gctcccaggt cctgcaaagc ctgttgcttg
     9061 tccgggatca gaccgttctc tatcccttcc gccagcgcca ttgcgatacc cgatgtccca
     9121 tgatacaagc ctccggagat actctgcaat cgctgcagaa attttgtacg gataatcttc
     9181 caaccatcgg tataagatgc gccgcaggag cggatgggat agccggaacg gtaggcccgg
     9241 gccaggagat acagtacgcc ggcaattccg gtatggaaac cggggtagat ggaagctgat
     9301 tgggattgat agtaatagaa cgctttattc tgaatggtac gggaccacca gacgtcttcg
     9361 ctgcccttca tgatggtggt gttgagggca ttcagcgcct gatcgatcgt ttcgccaaga
     9421 tccttttttt cgccatggcc aatgggattt gccgcgcggc cggggacgcc tgcagcaccc
     9481 ggagcgcccg cagcgcccgg agcgcccgca ccgccgggga cgagcacgaa ttgtcgcact
     9541 gttgttttca actcggacag atcgggtcgc aacgccggat cgtcgttgaa acaagcggcc
     9601 aggacaggca gcaaggcatg atccccgagg aagaatcgta attgttcgat cagcagttgg
     9661 gggttggtgg tagagaattt tcccggcagc aggttggtca gcaggacgat caggcttgcg
     9721 ccgaccgcgt agacatcttc tttctccgtg ggggtacggg attcacgctg ttcaggggac
     9781 atgaagcctt cggtgcctga gccgaagggt ggcaacgggt tcctgtttgt tttggaatag
     9841 accagctcga gatcgatcat cgtcagccgg ccgcccctgg tgacgatgaa attggctacc
     9901 gtgatatcgc gatgaataaa gcctttgcgg tgcatttttt cggcgatatc gatgagttgc
     9961 agggcataac ccaggagtct cagacggctc cgttgcggcg gcatccgcca gggtcgcccc
    10021 ttaaacaccg atgcgatctt ttcattcagc agggtacccc tgatatattc catggccagg
    10081 taggtacggt cgttctcttt gaacaattcg aaggctttcg ggacgggcag gctttcatac
    10141 aactcattct gcaattggta ttgccagatg agccggtcaa cgatgtcgcg ctcctgctcg
    10201 tcggcattca tgcaggggtt gccttctttc agcaggcacc attttatctt ccaaccctga
    10261 cccagcaaca gcgccttgtg gacatttccc ctggcgtcct ccttgagcgt cgccattgtc
    10321 ctgtatttat tgttcaggac tttgtttgcc gcgggagggt tggcctgcgc caggctggag
    10381 aatggccact gcaggccggg tgcgagcgta aaaggaatgg tataaggaat ttccggtgta
    10441 ccgttaatgg cgctaaaata gccatagcgg gtatatactc tgcctccaag gtggcggtcg
    10501 gtgaggatgt ggggcccttt gaaggtggat gtcaacgtcg taagcttcct ggcaagcgat
    10561 agtgccctgt tttcatcatc cgggaatatc gagatcacct tgcccagccg ggtatatccc
    10621 aatttgcctt ccaggatgga ttgggccgtt gtcttgtcgc gtgcgatctt gaacggcatg
    10681 ttctctccga tgagcagcgg aagcacggct tccaggaatt tttcgaattg ggatgccacg
    10741 acggaaagat gcaggatcca gcccggggcc gggcgagggt cgccaaccgt cagaaagagg
    10801 ttgtcctttt tgaaggtcag accgtgatgc cccaggatat taccgtagtc ttccatggct
    10861 gccgtgatca tatggatgtt ttagagtgaa tggtgatcat ccgggcgccg gccagcgcca
    10921 gcaaagacag gatgacgttc aggacgatat gggtcaccgg gggcagttgt tccaggaaac
    10981 cgccgcagcc gcagggcaga tcagggcccg aggaagtcag cgcgatcgtg tatatggtaa
    11041 agccgatcat gaggatcatg gacgcgtaga gaccggcgat gcgcagccgc ggaagcccga
    11101 gcataacggc aatggataat tcgacggccg gaacgaccca ggcgaaccgg gcgagattcg
    11161 ggccgatcag gggagactcc tccagctgac cgagaaaggc ctgccagtcg agtagcttgc
    11221 taacggctgt atagaggaac agcgcgatga acagccagct gatgaaatga tagatcgtcg
    11281 ttcttttcat acgggtagta atgggtggtt aagctgatca gggcgatgat agcgcagcag
    11341 gaaatggatg atcccgcagt tgccggtcag gagatcgggt gtgggatatg gggtatggtc
    11401 ctgcacccag taacgcgaac cgtcgccctg ctgccggtat tggtgttgca gctggcggac
    11461 gagccagccg gccttttctc tatattcttg cgaccggagg acccgtgcgg cctcgagcca
    11521 ggtttcgccg atgcctgcga gaccgctgga cagggtaaga tcgcggtcga cgtaaaaatc
    11581 gggaagagct ttgagtgccg attcggccag cagacggtaa atgggatcgc ctagcttttc
    11641 gtatcctttt aaaaaggcga ggatgatacc ggccagccct tcactgagtc cgggatcgga
    11701 agggtaattg cggcgcccca gccagtatag cgcacgggct gctgctttct gcgctgcgca
    11761 gtcctggaac cggccagacc aggccaggag gaaacagatg atgccggctg taccgtgtgc
    11821 aaaaccgggt agtttcatat gtcctttacc gtgacttccg gatatccatg aaccgtcccg
    11881 ttgctgtgcc gatatcagtt gttccacatt actgtgcaat aagagccata cccaatcgcc
    11941 gcccagcaga tcggcggcat tcagtgaaac cattccccgg cctgtcattc cgttggcgag
    12001 gtcccaatct tcggttgtcc cgttgagcag acaggactgt atatctccga ccgattgcag
    12061 ccgggtgact atcacattgt ttttcagacc ttcggtcata gccagcgcca tgcccgcggc
    12121 gccttcgaac catcctgccg gcagtcgggc gggcttttcg cattgaagat gcatgtaagc
    12181 gaggctgttt gcatactccg tcatgcagga atggatatcg aagcccgcct tttgcgcctg
    12241 tgcgaggagg tacaacacgc cggcgggcca gcatttcgcg ggacttgtca gcggcggcat
    12301 cgtcagcgcc tgcagagcgg aagatatagc ccgttcgatt ttaacggaat cgggttttcc
    12361 ggttgccgag agaggtacgg agcattgggg caactgttta tctctgaatt cccgaatgtt
    12421 ttttttgagc aaatggagac cgggtcgtct ggcggggcta tccagaaaac aggaagaaat
    12481 gatcgaaacc agcatgggat cggggataaa gaaacgtagc tgcatctgca ggatatgctg
    12541 gtagggggtg gaaaatttat tcgggatcag gccggtgaga agggcaacca gcgtcgctcc
    12601 gatggcataa atatcctcac cgctcgtagg aggttgcaca ttgtgttgtt ccggcgacat
    12661 gtatcccggt gttccgtagc ggaagggcgg tgaaggttgg tgaaggctgg tggaccaggc
    12721 cagctcgaga tcgatcatca ccagcccgtg tcgtgctaca agaaagttca aaggagtaat
    12781 atccctatga gcataacctt tatcatgcat ccgttgaatg atatccagca ggcggagcgc
    12841 atactccagc agtcgcagcc ggtggggctg cgatagccgc caccagggca tggatcggta
    12901 agtctcgagg atcacgtctt ccagacaggc gcctttgatg tactcgagta ccagaaaggt
    12961 gtcgccgccg gcctcgaaaa gatcgtaggc gcggggaagg gggaggctcc cctgcagatc
    13021 gcgatgcagc cggaactgcc agcgcagcct gtcgctggta tcgcgtccat catcatcggc
    13081 cgccatcgat tgtctgccct gtttgataac gcaccatttt atcttatacc atttttccag
    13141 ccaaagcgct tttttcacgt cgccacgcgg atcttcctta aggacggaga aggtcttgta
    13201 tttgccgggc agagcggttt cctgcgcgga tgtcgcccgg ggagcgaacc tgtcgaacgg
    13261 ccagtgaata tccgcgggca aatggaaagg caatttattg ggggagggtt ggtggcggac
    13321 atagagcagg cctcccaggt ggcggtcggt cgggatgcgg ggccctttaa atggtaaggt
    13381 cgcggcgatc agctgccccg ccagtgatgc cgcctgccga ttgttcaccg gatacagttt
    13441 gatgacctta ccgagtgctg tgggacctaa ccctccattc aggataagct gtgccatacc
    13501 cttgtttgcc gccaggcgga agcagacgtt ttccttcgcc agcaggggaa ggatcgtttt
    13561 cagcaattct tccaggctgg ccgcgatgac ggagacatgc agaacccatt ccgtcatctg
    13621 cggcatttgt ccgaccagca ggtaatagcc gtcggttttg aaactcagcc cgaattccgt
    13681 gaggatcgcc ggataagagc ctccggtatt cgttgcagca ggcagtgggg ggctacccgc
    13741 attatccgta atatgatata acatagggta cggcatgtgg attaaatttt gttattggta
    13801 aatggctttg aaaatattgt tcatctgtat ctgcctatca ccgagatagg tattcacgag
    13861 gcgtttatgg ctatcgtaga tggccatata ggggacagcg gcaggctgga agcttttata
    13921 gaaggaatac ccttcgtctt cggctacggt gatatttttg aaacggccga gatcaaagct
    13981 gtcatagaaa ccttttagct ccttgaaagg catgggggtc aggagataga tatgcgtaga
    14041 cttcaaggag tccatgttct taatgagcag tctggtatct gcctggcaat gccggcaatc
    14101 gggtctgaaa tacatgaaaa tgagcggagc gcctgacggt atcgcggcag aagcgagatg
    14161 cgtggtgctg tcgatgagca gcatcgtgaa ggccgggagg ctggtgatgt tgggaattac
    14221 cccggaccga tggcgacagg ccatgaaaca tgcgatagcg ccgatgagca gtatcttttt
    14281 catattgtct ggataaattt taaatgaaac ccggcggctt cagcatgttt gccgcgcttc
    14341 tcactgaaag ccagccgggt tgtttaagcc atcatctgaa caaaggtatc gcgggcggga
    14401 aggcgggtgt ttatgaatca gggataataa acaaactgat atgggttagt ccgaaaagct
    14461 gagaggaatg ttgatatttg ccaaatgaaa caacttctat ttatcgtcat ttgtctgcct
    14521 ttattcgtgg atgcacaacc ggcggccgtc gacacgcacg cgtggcagcc tgcgtcgctg
    14581 accttgccaa cccgttggag caagtccgtc tcaccttcca atgcgctgcc ggaatatccg
    14641 cgtccgcaac tggtgagggg tgagtggcag aacctgaacg ggctttggga ttattcgatc
    14701 acggattccg cttcggctga gcctaagtca tacgcagggc agatcctggt cccatatcct
    14761 ttggaatctg cattgtcggg ggtgaaggga gccttgcgac cggagcagcg gctatggtac
    14821 cggcgggacc ttgctatcgg gagcttacgc aaggagcgcc gctatctgtt gcatttcggc
    14881 gcggtggatt accaggcgac ggtctatgtg aatggaaagg cgatggggca gcatacaggc
    14941 ggctaccagg agttcaccat tgatatttcg ggtgctttgc acaaggggac caaccggctc
    15001 accgtggccg tgctcgatcc gaccgacaaa ggcaacaatc cgcatggcaa gcagacgctg
    15061 gatccgaaag ggatatggta tacgccgtcc agcggcatct ggcagaccgt gtggctggaa
    15121 accgttccga cggtatatat tgaaggtctt gaattgacac ccgatgtcga taatggggta
    15181 ttgaaggtaa aagcgaatgt gaaggccgcc gggaagggat ataccgtgga ggcggtggcg
    15241 ttttcggggt ctgcgatgac ggggagcgtc aagggtgcgc cggg
//
LOCUS       DASYSV010000179         5329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1057458, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000179 DASYSV010000000
VERSION     DASYSV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057458"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VGQ51_05435"
     CDS             <1..683
                     /locus_tag="VGQ51_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV9036037.1"
                     /translation="AIDYVYQDLHDFTGSFTVPADRTKPWGTAHAVLCAKNAIKEPFA
                     VINADDFYGRDAFEKAYKFLTGPVNPGEHCIIGYELAKTLSDNGTVSRGVCEVDGDGN
                     LVAIAERTKIYQEGDKITYEEGEKKYEVPFDSKVSMNFWGFDPSVFGYIEKLFGAFLK
                     EQGTNPKAEFFIPIIGDRFIKEGKGVIKVIPTSAKWFGVTYKEDAPGVQASLNALIDK
                     GEYPKKLW"
     gene            complement(736..1842)
                     /locus_tag="VGQ51_05440"
     CDS             complement(736..1842)
                     /locus_tag="VGQ51_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV9036038.1"
                     /translation="MQDTTLLDAVERYLRGEMSPEEKALFEHVRNTNPEVDQMVVEHS
                     LFLEHLNQYGETRRIKTQLNDIHHRLAETGQIEQSRPAIVVTLWNKYKRVVFVAACIA
                     GITALAISGLVSALTPKVDKAKIEYLDRRLVAQDYKLNKLSTQINTAGQTQTAPESYG
                     IGGTGFLIDGKGYLITAAHVVRGATRLKVQNPIGEYDAHIVQLDPHADLALLKINDTS
                     YHPFNGLPYGISKTSAELGEELFTLGYPRPDIVYNKGYLSARTGYQGDTTTYQITIAA
                     NPGNSGTPVLNNDGDVIGIVSSTQQNAQGMVFAVRSKNIFQALDSMKTDSALQKTDST
                     LSHLHIPLNSSLKGLDRQQQIRRIEDYIFIVKRN"
     gene            complement(1850..2407)
                     /locus_tag="VGQ51_05445"
     CDS             complement(1850..2407)
                     /locus_tag="VGQ51_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9036039.1"
                     /translation="MGTVTNEQDLLEGLAKNDRMAIETIYKQHYNMVQSMILANSGYP
                     DDARDIFQEAMIVLYEKVISGSFELNSQLKTYLYSVCRRLWLKRLSQLQRVSPEVEGL
                     EERIPVEEDLEKHEQRNRDFIVMEQAMNSLGEPCKSLLEAYYLGKKSMVEIAGDFGYT
                     NADNAKNQKYKCLMRLKKIFSQIKK"
     gene            2615..2815
                     /locus_tag="VGQ51_05450"
     CDS             2615..2815
                     /locus_tag="VGQ51_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036040.1"
                     /translation="MKKFFFVAATVFAFAACNNSASTEAKADSAKMSADSAKMSADSS
                     KMAADSTKMAVDSTKKDTTTKK"
     gene            complement(3068..4291)
                     /locus_tag="VGQ51_05455"
     CDS             complement(3068..4291)
                     /locus_tag="VGQ51_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9036041.1"
                     /translation="MLKDTTAPATTPTETVIRKTVFPILLAISFSHLLNDTMQSLIPS
                     TYPLLKRSLRLDFSQLGLITFCFQLTASLLQPFVGLYTDKKPQPYSLAAGMIFTGMGL
                     ALLSRAASFPVVLLAVALVGIGSSIFHPEASRLAYLASGGRRGMAQSVFQVGGNAGAS
                     FGPLLAAQIVAPYGQSNILWFGLFALTAFFVLLRTGAWYSRNLQRRRNKPAAHQIQTI
                     QFSRGRIAFALAILLVLIFSKYFYMASMSSYYMFFLIDKFHVSVRTAQLMLFIFLFAV
                     AVGTYIGGPVGDRIGRKYVIWFSILGVAPFTLILPYANLLWTGILSVCVGLILASAFS
                     AILVYAQELLPGKVGLISGLFFGLAFGMGGIGAAVLGNLADHTSIRHVFHLCAFLPLL
                     GLLTGFLPVPRRTSA"
     gene            complement(4302..4739)
                     /locus_tag="VGQ51_05460"
     CDS             complement(4302..4739)
                     /locus_tag="VGQ51_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036042.1"
                     /translation="MDRILVIDDLPLIAIAFKEVFRSVNPSALVGYCGNIYTALSAKT
                     YASATYDLVILGSEQEHWSTSLEQGMWELKDRFGVPRIMLYSAVYDAEIVGRMQAAGI
                     DAYVHRHESVEEIREAYRQLSAGTPFISGIFRALYYDYGHERK"
     gene            4821..>5329
                     /locus_tag="VGQ51_05465"
     CDS             4821..>5329
                     /locus_tag="VGQ51_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEV9036043.1"
                     /translation="MRVAINGMGRIGRLLTRRLLGPEGIELVAVNDIMDPENLAYLLR
                     YDSVYGTLPAPVSYAEGYLTVGDNMIAAFQSEDPAELPWGKLGVDIVLECSGRFTRGD
                     AAAIHLRSGARKVLLSTTGSPDIPLLIYGYNQDALKASTDIVSPGGCMTNCMTHLLNL
                     LNGIGIESA"
BASE COUNT         1206 a         1179 c         1684 g         1260 t
ORIGIN      
        1 tggcgataga ttatgtttac caggacctgc atgattttac gggttcgttc acggtgccgg
       61 cggacaggac gaagccctgg ggtacggcgc atgccgtgct ctgtgcgaag aatgcgataa
      121 aggaaccttt cgcggtgata aatgcggatg acttttatgg ccgtgatgct ttcgagaagg
      181 cttataaatt cctgacgggg cctgttaatc cgggcgaaca ttgcatcatc gggtacgagc
      241 tggcaaagac cttatcggac aatgggacgg tcagccgggg agtttgtgaa gtggatgggg
      301 acgggaacct ggtagccatc gccgagcgaa cgaagatcta ccaggaaggg gataagatca
      361 cttatgaaga gggggaaaag aaatatgaag ttccgttcga ctccaaggtg tcgatgaatt
      421 tctggggttt tgatccatct gttttcgggt atattgaaaa gctgttcggt gcttttctga
      481 aggagcaggg cacgaatccc aaggcggagt tctttatccc gataatcggg gatcggttca
      541 tcaaagaagg gaagggggtg atcaaggtga taccgacttc ggccaaatgg tttggagtta
      601 cctataaaga ggatgcgccg ggcgtacagg ctagcttgaa tgctttgatc gataaagggg
      661 agtatccgaa gaaattgtgg taatggccgt gtggcggcta gttccaaaga aaagggcctc
      721 gcggcccttt ttttattagt tgcgtttgac gatgaatatg taatcctcga tgcgtcgtat
      781 ctgctgctgc cggtcgagcc cctttaggga ggagttcagg ggaatgtgca gatggctgag
      841 tgtgctgtcg gtcttttgaa gcgcgctgtc ggtcttcatg gagtccagcg cctggaagat
      901 attcttcgag cggacggcga ataccatccc ctgtgcgttt tgctgggtcg agcttacgat
      961 gccgatgacg tctccgtcat tgttgaggac cggcgtgccg ctgttgccgg ggttggcggc
     1021 gatggtgatc tggtatgtcg tcgtatcacc ctggtatccg gttctggcgc tgagatatcc
     1081 tttattgtaa acgatgtcgg gtctcggata acctaacgtg aacaattcct cgcctaattc
     1141 ggcgctcgtc ttgctgatgc cataggggag gccgttgaac gggtgatagg aagtatcgtt
     1201 gatcttgagc agagcgagat cggcatgagg gtccaattgt acgatatggg cgtcatattc
     1261 gcctatcggg ttttgaactt tcagacgtgt ggcgcccctt accacgtgag cagcggtgat
     1321 gagatagccc ttgccgtcga tgagaaagcc tgtaccgccg atgccatagg attcgggagc
     1381 ggtttgcgtt tgtccggccg tattgatctg ggtagacagt ttgtttagtt tataatcctg
     1441 tgcgaccagg cgacggtcga ggtattcgat ctttgctttg tcgaccttag gtgtcagcgc
     1501 cgacacgaga ccgctgatgg ccagggccgt gataccggcg atacaggcgg cgacgaagac
     1561 gacgcgtttg tatttgttcc agagcgtcac gacgatggcg gggcgcgatt gttcgatctg
     1621 gcctgtttcc gcgagacgat ggtgaatgtc gttcaattgc gtctttatgc ggcgggtctc
     1681 tccgtattgg ttcaggtgtt ccagaaataa cgaatgttcg accaccatct ggtcgacttc
     1741 cggattggta ttccggacat gctcgaagag cgctttttcc tcgggggaca tttccccgcg
     1801 caggtagcgt tcgacagcat ccaatagggt agtatcctgc attttcacat tattttttga
     1861 tctgactgaa tatttttttc aaccgcatca ggcatttata tttctggttc ttggcgttgt
     1921 cggcgttagt ataaccgaaa tcgcccgcta tttcgaccat gcttttctta ccgaggtagt
     1981 aggcctcgag gaggcttttg catggttctc ccaggctgtt catcgcctgt tccatcacta
     2041 tgaaatccct gttgcgctgt tcgtgttttt caaggtcttc ctctaccggg atcctttcct
     2101 ccaggccttc cacttcaggg gagacccttt gcagctgact gagccttttg agccacaggc
     2161 ggcggcatac ggagtagagg taggtcttga gctggctgtt cagttcgaat gagcccgaga
     2221 tgaccttttc atagagtacg atcatagcct cctgaaaaat gtcgcgggcg tcatcggggt
     2281 agccactgtt ggcgaggatc atggactgaa ccatgttgta atgttgctta taaatagtct
     2341 cgatggccat tcggtcgttc tttgccagtc cttcgagtaa atcttgttcg ttggttactg
     2401 tccccacgct tatactttct ttaataccca aattaaacat tcgtaaccca aactaacggc
     2461 aaaaaaatca aaaaagagca atttagcctt caaaagatgg gtgatacgca ttaggttaaa
     2521 aaaaatgtaa aaaacgttga tttttgcggg ttactttttg ggaacacgtg gtattaacca
     2581 cgaaaccatt caattaacgt aaaaacaatc tcaaatgaaa aagttctttt ttgtagcagc
     2641 taccgttttc gcattcgcag cttgtaacaa cagcgccagc accgaagcta aggctgattc
     2701 cgccaagatg agcgccgatt ccgccaagat gagcgccgat tccagcaaga tggctgctga
     2761 ctctaccaag atggcagttg acagcacgaa gaaagatact actaccaaga agtaaagtta
     2821 tacaggtggg cttgtacacc taagttttta tggcaagcaa tcgttaggag gctatcctgt
     2881 tttcagggtg gcctctggcg tttaggggta tgtgaggcgg aggtgcggcg ggggcggaag
     2941 cggtgaaggg tgaggtggcg gagcgatggc gcgggggagc gtcgatggat gcggtggcgg
     3001 tgtgatggat gcggcggcag tgcgatggcg cggggagcgt cgatggatga ggtgccgagg
     3061 gtcgtgatca ggcggaggtg cggcggggta cggggaggaa gccggtgagc aagccgagga
     3121 ggggcaggaa ggcgcagaga tggaagacgt gccgtatgct tgtgtggtcg gcgagattgc
     3181 cgaggacggc tgcgccgatg cctcccatgc cgaaggccag tccgaagaag aggccggaga
     3241 tgaggccgac cttgccgggg aggagttcct gggcatagac gaggatggcg gaaaatgcgg
     3301 atgcgaggat cagtccgacg catacagaca ggatgccggt ccagaggagg ttggcgtagg
     3361 ggaggatgag ggtgaagggc gctacaccca ggatagagaa ccagatcacg tatttgcgtc
     3421 cgatgcggtc tccaaccggt ccaccgatgt atgttcccac agcgaccgcg aacaggaaga
     3481 tgaagagcat cagctgcgcg gtccggacgg atacatggaa cttatcgata aggaagaaca
     3541 tataatagct gctcatgctg gccatgtaga aatatttgga gaagatgagg accagcagga
     3601 tggcgagtgc gaaggcgatg cggccgcggg agaattgtat cgtctggatc tggtgggcgg
     3661 cgggtttgtt ccggcgtcgc tgcagattgc ggctatacca ggcgccggtg cggaggagga
     3721 cgaagaaggc ggtgagggcg aacaggccga accacaggat attcgactga ccatagggag
     3781 caacgatctg tgcggcgagc agcggaccga atgaggctcc ggcatttccg cccacctgga
     3841 agacggattg agccatgccg cgtcgtccgc cggaggcgag ataggcgaga cgcgaagctt
     3901 cgggatgaaa aatggaagag ccgatgccga cgagggcaac ggcaagcaat acaacgggga
     3961 agctggcggc ccgcgataac agggcgaggc ccatgcccgt gaagatcatg ccggcggcaa
     4021 gcgaataggg ttgcggcttt ttatccgtat ataaaccgac aaagggttgg agcagcgatg
     4081 ctgtcagctg aaagcaaaag gtgatgagac cgagctggct gaagtcgagt cttagcgacc
     4141 gtttgagcag tgggtaggtg gaggggatca gcgactgcat ggtatcgttg agcagatgcg
     4201 agaagctgat ggccagcagg atggggaaga ctgtttttcg gatgaccgtt tcagttgggg
     4261 tcgttgcggg ggctgtcgtg tccttgagca tggtttttga ttcatttcct ttcatggcca
     4321 taatcgtagt acagggcgcg gaagatgccg gagatgaagg gcgttccggc agataactgg
     4381 cggtatgcct cgcggatctc ttcgacggat tcatgacggt ggacataggc atcgatgccg
     4441 gcggcctgca tccttccaac gatctctgca tcataaacgg ccgaatagag catgatccgc
     4501 ggtacgccga aacggtcttt caattcccac atcccttgct cgaggcttgt cgaccaatgt
     4561 tcctgctcgg aacccaggat gacgaggtcg tatgtggcac tggcataggt ctttgccgag
     4621 agcgcggtat agatgttgcc gcaatacccg accagggccg atggattgac ggatcggaac
     4681 acttccttaa atgcaatagc gatcagaggc agatcgtcaa ttacgaggat acgatccata
     4741 ggtgtgggag ggtggtttgt tacagaggac atttgtgaca aaaatagcga tatttgtcat
     4801 tcgaaatcaa cgattgaacg atgcgggttg cgataaacgg aatggggagg ataggcagat
     4861 tgctgacacg gcggctgctc gggccggaag gcatcgagct ggtggcggtg aacgatatca
     4921 tggatcctga gaatttagcg tacctgttgc ggtatgattc ggtgtacggc acattacctg
     4981 caccggtatc ctatgcggaa ggatatctta cggtcggcga caatatgatc gcggctttcc
     5041 aaagcgagga cccggcggag ctgccctggg gcaaactggg tgtggatatc gtgctggaat
     5101 gttcggggcg gtttacgcgt ggggatgcgg cggcgatcca tcttcgttcg ggggcgcgga
     5161 aagtgttgtt atccaccacc ggatcgcccg atatacccct gctcatctat ggttacaacc
     5221 aggatgcgct gaaggcttcg acggatatcg tctcgccggg cggttgcatg accaattgca
     5281 tgacgcacct tcttaatttg ctaaacggga tcggtataga atcggcgca
//
LOCUS       DASYSV010000180         3804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_159611, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000180 DASYSV010000000
VERSION     DASYSV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159611"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VGQ51_05470"
     CDS             <1..295
                     /locus_tag="VGQ51_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV9036044.1"
                     /translation="ILQMYKDLDPVLNTPVRLAIVSALIKLKQADFAHLQETTATTQG
                     NLSHQIRRLQEAGYIEVIKTFKGNYPHTICKLTARGRKAFEQYVEDIRKYLHL"
     gene            381..1076
                     /locus_tag="VGQ51_05475"
     CDS             381..1076
                     /locus_tag="VGQ51_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036045.1"
                     /translation="MQNIRFRGPHLGILAVVFTALFIIGLSFVITFTPGAPHYPNLYD
                     AASRVTAYFREHPHDVLMCMFFQFLSAIPFGLFTVTAFNRLKFLGVKAVGPSIALLGG
                     LLVSLTMMVSSMIGWTMVFPGIASDDGALRALYYLGFALGGVGYSVPMGLLIAGIAVP
                     AGFMKLLPRWVVVFGIILGVIGESSCLALLFPNLTALIPLTRFPGFIWMMLAGFMLAR
                     RRPSPGGAIITAQ"
     gene            1125..>3804
                     /locus_tag="VGQ51_05480"
     CDS             1125..>3804
                     /locus_tag="VGQ51_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036046.1"
                     /translation="MTPKTRKRIIRWTLFPIAGIILLVGIAVAILYSQQQRLVKLAVG
                     ELNKRLPGELAVGGSDISIFQNFPYISIGLNHVQFYPNKQPGARPLYEAERLYVGFSL
                     PDILQQRYRVRAIALKNGHLDLIQQPDGQLNIVEASRMAPDTTATTEKAGPGLDLDIR
                     KLVLKNMTVSYFDPRQGQRFTTHIDRIQASLKYNDRLIDAGLDGKMLVDYTSSGDTTL
                     FRHKHVETNIRLSYEKATKLLRLPQGRITLEEAVFNISGMADLLHNNTVDLQITGDKP
                     DFKQLLSFAPDNVARELKHFRYDGHLSFDGKIKGPIGGGQQPLIELSFACSNAWLHNT
                     LAKKNLDSLAFKGYYTNGPGHSLQTSELRLLDMNARPGKGLFRGNFIMRNFADPKIMM
                     QVNSDLELAFIGDFLGIRDLQRITGRIQLKMNFKDLVDFSAPEKEIKALTEGIQSELK
                     VTDLSFRIPNYPYTIQQLNAHAIMKNGAVRLDTLFFHLGHSDFHLDGSLSDLPSLFHH
                     QEKPVMLRLGIHSGRMILKELLAKDSTVDKSVAEGADEEIRDLNLDLSLETSVDELRH
                     PAPLPKGIFKVDNFDASFKKYPHAFHDFGGTLTINDTSLLLRNLSGQLDSSDIRFSGR
                     INNYALWFSKVKKGKTVIAFDLKSKRLAINDLLSSTAYQYVPRDLQQEVATGIWLRSK
                     SELRYDSVFRFANIKIANISGLLQRHAIRMDSISGNIKFGTDHFVKIDTLKGRIGNSD
                     FNLSMRLYAGKDTVRRKKLNFLQFSSHLLDLDQLNRYLAEVETAQREEMTVLAADEPA
                     TPGPTPVTLAVAHDDGFNIFRIPFIDFNAAVNIDRLRYNKLGIKNLVTNLRMQSNQQL
                     YLDTLSLAMAGGTIRARAHFNGANPDKIYLRSRINVE"
BASE COUNT          870 a         1070 c          999 g          865 t
ORIGIN      
        1 aatcctccag atgtataagg acctcgaccc tgtgctcaat acccccgtgc ggcttgcaat
       61 cgtctcggcg ctgatcaagc tgaagcaggc ggatttcgcc catctgcagg agacgaccgc
      121 gactacgcag ggaaatctga gccaccagat ccgacggctg caggaggcgg gttatatcga
      181 ggtcatcaaa accttcaagg gtaactatcc gcacaccatc tgcaaactga ccgccagggg
      241 acgaaaagcc ttcgaacaat atgtcgagga tatcaggaaa tatctgcacc tgtgaaaagg
      301 cgccagtggt gccggggcta ttttttttgc gcatttggtt tgtggcgcaa actatattaa
      361 tcaactttaa aacgacaatt atgcaaaaca tccgattcag gggcccgcat ctgggcatcc
      421 tcgcggtcgt cttcaccgcc ttgttcatca ttgggctatc ctttgtgatc acctttactc
      481 ccggcgcccc gcactatccc aacctttatg atgccgcctc acgggttacc gcctatttcc
      541 gggaacaccc gcacgacgta ctgatgtgca tgttctttca gttcctgtcc gccattccat
      601 tcggcctgtt tacggtcacc gctttcaatc gcctgaaatt tctgggtgta aaggctgtcg
      661 gtccgtccat cgccctgttg gggggactgt tggtttcatt gacgatgatg gtctcttcca
      721 tgatcggctg gacgatggta tttccgggaa tagcgagcga tgacggggct ctgcgggcac
      781 tgtattatct gggtttcgca ctcggcggag tgggttattc ggtccctatg ggcctgctga
      841 tcgccgggat tgccgtgccg gcaggtttta tgaaactcct tccccgttgg gtggtggtgt
      901 tcgggatcat cctcggagtg atcggcgaga gcagctgtct ggcgttattg ttcccgaatc
      961 tgacggcttt gatccctttg acgcggttcc cgggttttat ctggatgatg ctggccggtt
     1021 ttatgctcgc cagacggaga ccctcccccg gcggcgccat tatcactgcc caatgatccc
     1081 cgaattatac tttatcttgc ggtcccatcg ttttatttct acctatgacg ccaaaaacac
     1141 gcaaacgaat aatccgttgg acccttttcc ctatcgctgg catcattctc ctggtcggta
     1201 tcgccgtcgc catcctgtat tcccagcaac agcggttggt caaattggca gtgggcgagc
     1261 tgaacaagcg gcttcccggt gagctcgcgg ttggcggcag cgatatctca atttttcaga
     1321 atttccccta tatctctatc gggttgaacc atgtgcagtt ctaccccaac aagcaacccg
     1381 gcgcccgacc gctctatgaa gccgagcgac tttatgtcgg cttcagcctc ccggatatcc
     1441 tacagcaacg ataccgggtc agggccatcg cgttgaagaa cggccatctc gatctgatcc
     1501 agcaacccga cggtcagctg aatatcgtcg aagccagccg catggctccc gataccaccg
     1561 ccacgacaga aaaagccgga ccgggtctgg acctcgacat ccgcaagctg gtgttgaaga
     1621 atatgacggt atcctatttc gacccccgtc aggggcagcg gttcaccacg catatcgatc
     1681 ggatccaggc atcattaaag tataacgacc ggttgatcga cgccggtctc gatggtaaaa
     1741 tgctggtcga ttatacgagt tccggagaca ccacgctgtt ccggcacaag catgtcgaaa
     1801 cgaatatccg gctttcctat gaaaaggcta caaagctgct ccggctgccc caggggcgca
     1861 tcacgctgga ggaagccgtt ttcaatattt cgggtatggc cgatctgctg cacaacaata
     1921 cggtggacct gcaaatcacg ggcgataaac cggatttcaa acaactgttg tcgttcgcgc
     1981 ccgacaacgt ggccagggag ctgaaacatt tccgttatga cggtcatctc agctttgacg
     2041 ggaagataaa gggtcccatc ggcggtgggc agcagcctct tatcgaactc tctttcgcgt
     2101 gcagcaacgc ctggctccat aatacgctgg ccaaaaagaa cctcgattcc ctggccttta
     2161 aaggttatta taccaacgga ccgggacatt cgctgcaaac ctccgagctt cggctgctgg
     2221 acatgaacgc ccggcccggg aaggggcttt tcagaggcaa tttcatcatg cggaatttcg
     2281 ccgatccgaa gatcatgatg caggtgaact cggacctgga gctcgcgttc atcggcgact
     2341 tccttggtat ccgcgatctg cagcggatca ctggccgtat tcagctgaag atgaatttca
     2401 aggacctggt ggatttcagc gcgccggaaa aagagatcaa ggcgctcacc gaaggcatcc
     2461 aaagcgaatt gaaggtgacc gatctcagct tcaggatacc caattatccc tatacgatcc
     2521 aacaactgaa cgcgcacgcc atcatgaaga acggcgccgt gcgactcgat accctgttct
     2581 ttcatctcgg acattccgat ttccatctgg acggatcgct gagcgacttg ccctcgctgt
     2641 tccatcacca ggaaaagccg gtaatgctgc gcctgggtat ccacagcggc agaatgatcc
     2701 tgaaggaatt gctggcaaag gattcgaccg tcgataaaag tgtggccgaa ggggcggacg
     2761 aagagatccg ggatctgaat ctcgatcttt cgctggaaac ctcggtggac gagctccggc
     2821 atccggctcc gctgcccaaa gggatcttca aagtcgacaa tttcgatgcg tcgttcaaaa
     2881 aatatccgca tgcctttcac gatttcggcg gcaccctgac catcaacgat acatcccttc
     2941 ttctgaggaa cctcagcggg cagctggaca gcagcgatat ccgattcagc ggccggatca
     3001 acaactacgc cctgtggttc tccaaagtga aaaaaggcaa gacggtgatc gctttcgatc
     3061 tgaaatcgaa acggctggcc ataaacgatc tgctcagcag cacggcttac cagtatgtcc
     3121 ccagggatct ccagcaggag gtcgccaccg gcatctggct ccggtcgaaa tccgagctcc
     3181 ggtatgactc ggtgttccgg ttcgccaata tcaagatcgc caacatctcg ggcttgctgc
     3241 agcggcatgc catccggatg gacagcatca gcggtaatat caaattcgga accgaccact
     3301 tcgtcaagat cgatacgctg aaaggccgta tcgggaacag cgatttcaac ctgtcgatgc
     3361 ggctttatgc cggaaaggat accgtccgcc ggaagaaact gaacttcctt caattcagct
     3421 cgcatttact ggatctggat cagctgaacc gatatcttgc ggaagtagaa acggcccagc
     3481 gtgaagaaat gaccgtgctt gcggcggatg agccggcgac tccaggtccg acgccggtga
     3541 cgctggcggt ggcccatgac gatggcttca acatcttccg cattcctttt atcgatttca
     3601 atgctgccgt caatatcgac aggctgcgat acaacaagct ggggatcaaa aacctggtga
     3661 caaacctgcg catgcaatcc aatcagcagc tgtacctgga cacgctgagt ctggcgatgg
     3721 cgggcggaac gatcagggcg agggcgcatt tcaatggcgc caatccggat aagatctatc
     3781 tcagaagcag gatcaatgtc gaag
//
LOCUS       DASYSV010000181         7384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_805501, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000181 DASYSV010000000
VERSION     DASYSV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805501"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..704
                     /locus_tag="VGQ51_05485"
     CDS             369..704
                     /locus_tag="VGQ51_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036047.1"
                     /translation="MSPLTLRTDFGCTEAVIDDDCGLKRFYEVANILSDDLDIKFTQK
                     TDDFDSLMWDFLYKGHILTLHYNIYTGISIYPRKFREAPRKDNDAVVEVAKFLESKLL
                     INTARRFMS"
     gene            859..2016
                     /locus_tag="VGQ51_05490"
     CDS             859..2016
                     /locus_tag="VGQ51_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV9036048.1"
                     /translation="MTPARPTPFYTRLAMVLISLIALVYIAILGKPVLAPLIFGLLFS
                     VMLLPVANFLERRCRLPRSLASLLSVLLLVISIAALFYLVGSQISSLADDWPQFKEQV
                     ISSIDDMQHWISLKFHIRIKQQNTYVNNATSKLLESGGTVLSTAVASVSSLLLSIVFI
                     LFDTFFLLYYRRLIIRFLVAVFKQENAVVVYDILAQVQSRIRQYILGLLLEMVVVASA
                     TCLGLWIIGVNYAILLGLLTGLFNIIPYIGILTALLLSVLVTFAAAGAAKLLLVIAVV
                     LGIHLIDSNVLLPVIVGSKVRINAFITVLGVIIGESIWGIDGTFLAIPVIAIGKIVFD
                     RIESLQPWGLLLGDEIDEKQPPPLKEEIKEEGHDVTVAPERPDNPAGPTAQ"
     gene            complement(2112..3140)
                     /gene="rsgA"
                     /locus_tag="VGQ51_05495"
     CDS             complement(2112..3140)
                     /gene="rsgA"
                     /locus_tag="VGQ51_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV9036049.1"
                     /translation="MTGKVYKSTGSWYVVKTAEGGVWNARLKGLFKLDGITSTNPLAV
                     GDDVEFEIESEDEGTAIITKIHERSNYINRQSPSHRNQHHIVAANVDQSLLIVTLREP
                     RTSQGFIDRWLLTCEAYHVPAVLLFNKSDNYGKKEEARFEEWKAMYEAIGYRVIRMSV
                     VSGDGLEEARALLAGRISLLSGHSGVGKSSFLNALFPEMRLRTHEISGWSGKGTHTTT
                     FAEMFDLPGGEGSEGGGGGERQAGGGEAGGGGGESEASGGEAGGGRIIDTPGMREFGL
                     VDISKQELSGYFPEMRALAGQCHFNNCLHLEEPGCRVKEAVGEGRIAAERYDSYCAIL
                     ESIGKDEY"
     gene            complement(3142..4146)
                     /locus_tag="VGQ51_05500"
     CDS             complement(3142..4146)
                     /locus_tag="VGQ51_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV9036050.1"
                     /translation="MLYDWYQHIYFGQPIFFWLLLLVPLLIWWELKRDSAGQATVMVS
                     SVRAFRGTRSWKNFLRPLLFILRLLTLVCLIIALARPQTHNDEQITNGEGIDIVLCLD
                     ISGSMLAQDFSPNRMEAAKNVAAEFIDNRPTDRIGLVIFSGEAFTMCPLTTDRNMLKS
                     QLYSVQSGLLEDGTAIGSGLATGVDRLRNSPSKSKVIILLTDGENNGGLIDPNTAKEI
                     AKSLGVKVYTVGMGTEGFAPVPVQTPNGVVMQREKVNIDEKLLTQIANETGGHYYRAK
                     DNESLKDIYAEINRLEKSKIEITTNRRYAEQFFPFALAAALALFLELLLRWTVLRKFP
                     "
     gene            complement(4172..5218)
                     /locus_tag="VGQ51_05505"
     CDS             complement(4172..5218)
                     /locus_tag="VGQ51_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036051.1"
                     /translation="MLLQAQHRHKPVAPPTPPATVQASTDVQKIFIGQPIRLTLEATV
                     PENVDFTWPEIDSLAHFEQLDSGKFDTVVLPGARTYRESITFTSWDSGSWAIPRLSFL
                     AGGKKVLTDSIRILVDYTKDSTKDFHDIKDIVEVPNPFEKYFGWIVAGFTLISLVLVV
                     WLVRKKKLLKTWIPRGEVVRLSPYEEALQQLDQLQKQQLPEAGKFKTHYSRLGEILRE
                     YLLRRLGISTHAETSEELIGEIRRHELLPPVLYDALAEGLRMSDFVKFAKYQPGISES
                     GQHYDAVRGAIEAMEQRARGEEERRRSEGQENRGDRGGAGSTGVQGNPGSQGGPGDKA
                     IPEPLEGARPKDRH"
     gene            complement(5275..6177)
                     /locus_tag="VGQ51_05510"
     CDS             complement(5275..6177)
                     /locus_tag="VGQ51_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV9036052.1"
                     /translation="MLTTAEILKKVRELEIKSKKLTRHLFTGEYHSAFKGRGMSFREV
                     REYAPGDDIRFIDWNVSARFNHPFSKLFEEERELTVMLLVDVSASSLFGTVHARKKDL
                     ITEICAVLAFSAINNNDKVGVIFFSDKVEGYIPAKKGREHVLYIVRQLLTIDPKRKGT
                     NLGEAIRFFNRSARHKAIVFLLSDFLDGKGDPSARPSYEEALKVAGKKHDMIGIKVYD
                     KMDMQLPGIGMIEAEDSETGSRYWVDTNDYLVRTNYQQYFFNQTEQCKSIFQKAGCDL
                     LHLRTDEDYVKILQRFFVGRHRPG"
     gene            complement(6391..>7384)
                     /locus_tag="VGQ51_05515"
     CDS             complement(6391..>7384)
                     /locus_tag="VGQ51_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV9036053.1"
                     /translation="TAEDIRQLNSKIQYQAAFIDRLRDEVGRVIIGQHQMLDRLLIGL
                     LSNGHVLLEGVPGLAKTLTIKSLAQAIHAKFSRIQFTPDLLPADVIGTMIYNQQKHEF
                     VVRKGPIFANFILADEINRAPAKVQSALLEAMQERQVTLGDTSHLLEEPFLVLATQNP
                     LEQEGTYPLPEAQQDRFMMKVIVDYPSKQEEQLIIRQNVQGAQASKVEQVVSIQEILQ
                     AKDLARQIYMDEKVEHYILDIVFATRTPEKYKLEKLKPLIAYGGSPRASINLALAAKA
                     NAFLSKRGYVIPEDVRSISRDVLRHRIGLTYEAEAESVNVENVIDEVLKAIPIP"
BASE COUNT         1581 a         2396 c         1633 g         1774 t
ORIGIN      
        1 aaatccaccc ccaaaccccc tgaatcccac atttccggag ttaaattata ggggtatacc
       61 ttgcttttat aggataatat ttgtaggttt aaatacgttt ttacctaaca atccgatgaa
      121 accgaaccct ggctatagaa acaccttcgc tatcattctc cttacatttc tgctcaccgg
      181 cttttcttcc tgtggatggg tgaataacct ttttggaccg aagtacggat gcccaagcaa
      241 tggcaaaaat gtcggcgcgg aacggatact cagtggcgaa aaagtgccaa aagctggtaa
      301 gttcaaaagc tgaaaccaga taccccgaaa aatcaatacc ctttaaataa acacaaaatc
      361 cctacactat gagccctcta actcttcgca ccgatttcgg ttgcacagaa gccgtaatag
      421 acgatgattg cggactgaag cgattctatg aagtcgctaa cattttatcc gacgacctgg
      481 acattaaatt cactcaaaaa accgacgatt tcgactcgct gatgtgggac ttcctttaca
      541 aaggacatat cctcacactg cattacaaca tttacaccgg catctcgatc tacccccgca
      601 aattccggga ggcgccgaga aaagacaacg acgccgtggt ggaagtggcg aaattcctcg
      661 aaagcaaatt actcatcaat accgcgagac gctttatgtc ttaacctctt ccaagagggc
      721 gctaacgatt gtttgcagca tgttgaggac ccgcgacgac cgcgggtctt ttctttgcgc
      781 gacggcctgt ggcggcccgc cttttttcgc cccgcctgcc ccccgcacct catcgctttt
      841 ttccctatct tgctattcat gacacccgcg cgcccaactc ccttctacac aaggctggcc
      901 atggtcctga tcagcctgat cgccctggtc tatatcgcca tcctcggaaa acccgtcctc
      961 gcgccactga tattcgggct gcttttctcc gtcatgctgc tgcccgtcgc caatttcctc
     1021 gaaagacgat gccgtctgcc aaggagcctt gcgtcgctct tatccgtact gctcctcgtc
     1081 atttcgatcg ccgccctttt ttatctcgtc ggctcgcaga tcagctcgct ggcagatgac
     1141 tggccgcaat tcaaagaaca ggtcattagc tccatcgatg acatgcagca ttggatctcc
     1201 ctgaaattcc atatccggat caaacagcaa aacacctatg tcaataacgc gacgtcgaaa
     1261 ttactcgagt ccggcggtac cgtgctgagc actgccgtcg cctctgtctc ctccctactg
     1321 ctgagcattg ttttcatcct cttcgacacc tttttcctgc tctactaccg ccggctgatc
     1381 atccgcttcc tcgtagccgt cttcaaacag gaaaatgccg ttgttgtcta tgacatcctc
     1441 gcacaggtgc agtcccggat ccgtcagtat atcctcggcc tgctgctcga aatggtcgtc
     1501 gtcgcgtcgg ctacctgtct tggcctctgg atcatcggcg tgaattatgc catcctcctg
     1561 gggcttctca ccggcctgtt caatatcatt ccctatatcg gcatcctcac cgccctcctt
     1621 ctttccgtcc tggtgacctt cgccgcggca ggggctgcaa agctgctgct ggtcatcgcg
     1681 gtcgtcctgg ggatacatct tatcgacagc aatgtgctcc ttcccgtgat cgtgggctcg
     1741 aaggtgcgta tcaatgcttt tatcacggta ttgggcgtca tcatcggaga atccatctgg
     1801 ggcatcgacg gcaccttcct ggccatcccc gtgatcgcga ttggcaagat cgtattcgat
     1861 cgcatcgagt cgctccaacc ctggggcttg cttttgggtg atgaaataga tgagaaacag
     1921 ccgccgccgc tgaaagaaga gataaaagaa gaaggtcatg acgtgaccgt cgctcccgaa
     1981 cgcccggaca accccgcagg acctacggct caataatcgc cctccgcgtc ctcaacaccc
     2041 cgcggccccg ggcgaccacc tcaataatcg ccctccgcgt cctcaccacc ccgcggtccc
     2101 cggcgaccac ctcaatattc gtccttcccg atactttcca ggatcgcgca ataactgtca
     2161 tatcgctccg ccgctatcct cccctcgccc accgcctcct tgacccgaca ccccggctcc
     2221 tcgaggtgaa gacaattgtt gaagtgacat tgccccgcca acgcgcgcat ctccggaaaa
     2281 tatcccgata attcctgttt gctgatatcg accagcccga attcccgcat gcccggcgta
     2341 tcgatgatac ggccaccgcc cgcctcaccc ccactcgctt cactctcgcc gccacccccg
     2401 cccgcctcac ccccgcccgc ttggcgctcc ccgccacccc cgccttcgct cccctccccc
     2461 ccaggcagat cgaacatctc cgcaaacgta gtcgtatgcg tacccttgcc gctccacccg
     2521 ctgatctcat gcgtccgcaa acgcatttcg ggaaacaacg cattcagaaa tgacgacttc
     2581 cctacgcccg aatgcccgct taacaaactg atccgtcccg ccagcagcgc acgcgcctct
     2641 tccagcccat cgcccgaaac tacgctcata cggattaccc ggtagccgat ggcttcatac
     2701 attgccttcc actcttcaaa acgcgcctcc tcctttttac cgtaattatc actcttattg
     2761 aacaaaagca cggctggcac atgataggcc tcacaggtca gcaaccacct gtcgataaat
     2821 ccctgcgagg tccgcggctc gcgcaaagtc acgatcagca ggctttggtc gacattcgcc
     2881 gctactatat gatgctggtt acggtgtgaa ggactctgac ggttgatata attgctgcgc
     2941 tcgtggatct tcgttataat ggccgttcct tcgtcttcgc tttcgatctc gaactccaca
     3001 tcgtcaccca cggccagcgg attggtcgac gtaatcccat ccagcttgaa aaggcctttc
     3061 agccgggcat tccatacccc tccttcggcc gtcttcacga cataccagct tccggtcgat
     3121 ttgtatactt taccagtcat ttcacggaaa tttgcggagc accgtccatc tcagcagcaa
     3181 ctcgaggaac aacgccagcg cggcagccag cgcaaaagga aagaattgct cggcataccg
     3241 ccggttcgtg gtgatctcga tcttcgattt ttcgagacga ttgatctcgg catagatatc
     3301 tttcagactt tcattgtcct tggccctgta ataatgccca cccgtctcat tagctatctg
     3361 cgtaagcagc ttttcatcga tgttaacctt ttcgcgttgc atgaccacac cattcggcgt
     3421 ctgtaccggc acgggggcaa atccttccgt acccataccg acggtatata cctttacacc
     3481 cagcgacttg gcgatctcct tggccgtatt cggatcgatc agtccgccgt tattctcgcc
     3541 gtccgtcagc aggatgatca ccttgctctt cgaaggagaa ttacggagcc ggtcgacgcc
     3601 cgtagccaaa cccgatccga tagccgttcc gtcctccaac aacccactct gcacactgta
     3661 cagctggctc ttgagcatgt tgcgatccgt cgtcagagga cacatggtga acgcttcgcc
     3721 cgaaaagatc accagcccga tacggtccgt cggccggttg tcgataaact cggcggctac
     3781 gtttttagcg gcctccatcc ggttgggaga aaaatcctgc gccagcatgc tcccgctgat
     3841 gtccagacac aggacgatgt cgatgccttc accgttggtg atctgttcgt cattatgcgt
     3901 ttggggacgc gccagtgcaa tgatgagaca gaccagcgtc agaagacgca gtatgaatag
     3961 caggggacga aggaaattct tccaggaccg cgttccgcga aaagcacgca ccgaagacac
     4021 catgacagtc gcctgacccg cactatcgcg cttcagctcc caccagatca gcagcggcac
     4081 cagcagcaac aaccagaaga atatcggctg cccaaaatat atatgttgat accaatcgta
     4141 tagcacgaat atcttaattg ctttgttgta tctaatgtct gtccttcgga cgagcccctt
     4201 ccagcggctc aggaatcgcc ttatctcccg ggccgccctg gcttcctggg ttcccttgca
     4261 ctcccgtgct ccccgcgcct ccccggtcgc cccgattctc ctgcccttcg ctcctgcgtc
     4321 tctcctcttc tccccttgcc cgctgctcca ttgcctcgat agccccccgc accgcatcat
     4381 aatgctgccc gctttcgctt atccccggct gatactttgc gaatttaacg aaatcgctca
     4441 tcctcaagcc ctctgccagc gcatcataca ggacaggagg cagcaattca tgccggcgta
     4501 tctcgccgat cagctcttca ctggtctcgg cgtgtgtcga aatacccaat ctccgcaaca
     4561 agtactcccg gagtatttct cccaaccggc tataatgcgt cttgaacttt ccggcttcgg
     4621 gcagctgctg cttttgcaac tgatccagct gctgcagggc ctcctcatag ggcgacaacc
     4681 gcacgacctc accgcgcggg atccaggtct tcagcagttt ctttttgcgg accagccaga
     4741 cgaccagcac cagcgagatc agcgtaaatc ccgccacgat ccaaccgaaa tacttctcga
     4801 aaggattcgg cacttcgacg atgtctttga tatcatgaaa atccttcgtg gaatccttcg
     4861 tataatccac caatatgcgg atggagtcgg ttaagacctt ctttccgccc gccaggaacg
     4921 acaatcgcgg tatcgcccag gaacccgaat cccaactcgt aaaggtgatg ctttcccgat
     4981 aggtccgcgc gcccggcaat actaccgtat cgaatttccc gctgtccaat tgttcaaaat
     5041 gcgccagcga gtcgatttcg ggccaggtga aatcgacatt ttcgggcacc gtggcctcca
     5101 gcgtcagccg tataggttgc cctataaata tcttctgcac gtcggtggac gcctgcaccg
     5161 tcgcaggcgg agtgggcgga gccacaggct tgtgcctgtg ctgtgcttgt aacagcatca
     5221 ccggcaggca gaaaaaaagt atgagggcgt atgttcttaa gggtctcatg gggcttatcc
     5281 gggccggtgg cgaccgacaa aaaacctttg caggatcttc acatagtctt cgtcggtccg
     5341 caggtgcagg agatcgcacc cggccttttg aaagatgctt ttacattgct ccgtctggtt
     5401 aaaaaaatat tgctgatagt tggtgcggac caggtaatcg ttcgtatcta cccaataacg
     5461 gctgcccgtc tccgaatcct ccgcttcgat catcccgatc cccggcagct gcatatccat
     5521 cttgtcatac accttgatgc cgatcatatc atgttttttc cccgccacct tcagcgcctc
     5581 ctcataactc gggcgggccg acggatcacc tttcccatcc agaaaatcgc tcaaaaggaa
     5641 tacgatggcc ttatgccgtg cgctgcgatt gaagaatcgg atcgcttcgc ccagattggt
     5701 cccctttctc ttcgggtcga tggtcagcag ctgtctgacg atataaagaa catgttccct
     5761 tcccttcttt gcaggtatgt atccctccac cttatcgctg aaaaagatca cgccgacctt
     5821 atcgttgtta tttatcgccg aaaaagccag caccgcgcat atctccgtga tcaggtcctt
     5881 cttacgggca tggacggtac cgaaaagcga gctggcgctg acatccacca gcagcatgac
     5941 ggtgagctcg cgctcttcct cgaacagctt gctgaacgga tggttgaatc ttgccgagac
     6001 attccaatcg ataaaccgga tgtcatcccc cggcgcatac tcccgcacct cgcggaagct
     6061 catgccgcgc cccttgaagg cgctatgata ttctcccgtg aacaagtgcc gggtgagctt
     6121 tttgcttttt atctcgagct cccgcacctt tttcaatatt tcggctgtcg tcagcataat
     6181 tttttaatca ttcatccgcc ccgcgcgccc catcatccgc ccagcctcgc ggccttcctg
     6241 ccaccgctca cccgcccccg gacgctccgg gatcattcat ccgccccgcg cccccgatca
     6301 tccgcccagc ctcacggcct ccccccacgc tcatccttcc cgcctcccca ggccttcccg
     6361 ccccacgcct caaccatccg gcaccccacc tcaaggtatc gggatcgcct tcaacacttc
     6421 atcaataaca ttctccacat tcacactctc cgcctcggcc tcatacgtca acccgatacg
     6481 atgtctcaat acatccctcg agatcgaacg cacgtcttca ggaataacat atcctctttt
     6541 acttaggaac gcattggcct tggccgcaag cgcgagattg atgctcgccc gcgggctccc
     6601 gccataggcg atcagcggct tcagcttttc caatttgtat ttctccggcg tccgcgttgc
     6661 aaaaactata tcaagaatat agtgctccac cttttcatcc atatagatct gccgtgcaag
     6721 atccttagcc tgaagtatct cctgtatgga caccacctgc tccaccttcg aagcctgcgc
     6781 tccctgcaca ttctgacgga tgatcagctg ttcttcctgt ttggagggat aatcgacgat
     6841 caccttcatc atgaagcggt cctgctgagc ctcgggcaac ggataggtgc cctcctgttc
     6901 caacgggttc tgtgtggcga gcacaaggaa cggctcctcc agcagatgac tcgtatcccc
     6961 cagcgttact tgtctctcct gcatcgcctc cagcaatgca ctttgcactt ttgcaggcgc
     7021 ccggttgatc tcatcggcca atataaaatt ggcgaagatc ggtcccttcc ggaccacgaa
     7081 ctcatgcttt tgctggttat agatcatcgt accgatgacg tctgccggca acaagtcggg
     7141 cgtaaactgt atacgactga acttggcatg aatagcctgc gccagcgatt tgatagtcaa
     7201 tgtcttagca agacctggta caccttccag caaaacatgg ccattactca acaggccgat
     7261 cagcaaccgg tccagcatct gatgctggcc gatgatcacc cttcccacct catcccgcag
     7321 cctgtcgata aaggctgcct ggtactggat cttactgttc aattgtcgga tatcctctgc
     7381 tgta
//
LOCUS       DASYSV010000182         7347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_159739, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000182 DASYSV010000000
VERSION     DASYSV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159739"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VGQ51_05520"
     CDS             <1..515
                     /locus_tag="VGQ51_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9036054.1"
                     /translation="PKMATARALPAIASVFRRYNPGTVFDYTFNDADYAKKFDFETRI
                     GSLATFFAAFAIFISCLGLFGLASFMAEQRTKEIGVRKVLGASVLHLWAMLSKDFLLL
                     VSISFLVAIPVSWYIMHGWLQQYDYRTSISVWIFVMTAVAALAITLVTVSFQSIRASL
                     ANPVRSLRSE"
     gene            complement(595..3027)
                     /locus_tag="VGQ51_05525"
     CDS             complement(595..3027)
                     /locus_tag="VGQ51_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036055.1"
                     /translation="MLKNYVLIAFRNLTRHRFYSVLNIAGLAAGMAVSLLIGLWVYNE
                     VTYNYYHRNHSRLGIVYDTQVSNGAMHTENSVDIPLADQLRQEYGDQLKHVALATPYE
                     AHLVSPGQASDKKIERTCRWVEPDLPVMLTLHLLKGSADCLRDPSSALLTASMARSLF
                     GTADPMGRTVLLDSRLPFKVAGVYEDVPENTDLHGVDFLLPWEKYAETHSWIRGSQHG
                     WGNYYARIFVQLNDGVDFDRLSERIAYIPRRHLTNSKDSVFIQPMDRYHLYGNFKNGA
                     PAGGQIRFVRMFSIIALSILLLACINFMNLSTARSERRAREVGIRKTLGSLRGQLIGQ
                     FLGESVMISLLSLGFGLLLALLLLPMFNGMARRHLKIPWGEPYFWLIALGFSLFTGLV
                     SGSYPALYLSRFQPVKVLKGDLTRSRTRPSRWLGRSFMRAGRNAALPRKILVVIQFTV
                     SVFLILGTLVIYRQIQYARNRPTGYVREGLLNVNMLNNSGLRFRYNAIRSDLLASGAA
                     QDVTASNGPMTDTWSVRGGLTWKGKDPYANPQFGWVSITHDYGRTVGWQILQGHEPSR
                     SLSGDSIGLVMNEAAVRLAGFRDPIGQRITVDSISRPVIAVVKDLVMDSPYDPALPTI
                     FFMDYSWFNYLTIRLNRNMRPADAMTRIQQVFRKYDPDGLVSYQMADEAYAQNFDVEQ
                     RVGEVSTFFTVLAIFISCLGLFGLASFVAEQRTREIGLRKVLGASVFNLWNTLSREFV
                     MLVIIACAIAIPLSGWLMHEWLQKYAYRTELSWWLFASAAGGALIITLLTVSYQTVRA
                     AMANPVKSLRTE"
     gene            complement(3046..3615)
                     /gene="msrA"
                     /locus_tag="VGQ51_05530"
     CDS             complement(3046..3615)
                     /gene="msrA"
                     /locus_tag="VGQ51_05530"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEV9036056.1"
                     /translation="MSASQPPATHPTDIVTFGTGCFWCTEAIFQELKGIVSVTSGYMG
                     GHGENPNYEEVCSGTTGHAECLQLAYDPAVISYDELLQVFFESHDPTQLNRQGNDIGT
                     QYRSAIFYHTEEQKQKAEHYKAELDKAGAYPNPIVTEITPAGPFYPAEDYHQNYYNSH
                     NSQPYCYVVIRPKVEKFEKAFKNKLKKER"
     gene            complement(3621..4319)
                     /locus_tag="VGQ51_05535"
     CDS             complement(3621..4319)
                     /locus_tag="VGQ51_05535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV9036057.1"
                     /translation="MLVQLLLSWILLHFIERRNLEALGIRPTARRMQLAGIGFGLAMI
                     YMIAYQLGEAAIQHAPYHRNEAYGLPQLGLAMWYLFKSVVFEELIFRGALLYILIRRI
                     GSAKAVLTSAVAFGIYHFFVVGIHSPLQVVILFLTTGLMGYVLAAAFVRTGSIWVPIA
                     IHYSLNFTPYVGFSQSNGMGNQLFVHTAPLHQPTIQTLGLLVIHNFIFPLVVLWWLRS
                     IRPERELKPQTYPN"
     gene            4384..4914
                     /locus_tag="VGQ51_05540"
     CDS             4384..4914
                     /locus_tag="VGQ51_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV9036058.1"
                     /translation="MKNRQLSTGRDVRDGEIREAGPHAPDGEIGVTGPTARDMEVAAA
                     LRTGVHRLVKLLRRETRNEAQLSLTERSTLGLLYPDLQLAPSEIARMEKVTTQSMSQV
                     INYLMELDLIVRTPSGEDKRKVLLSLTPAGRVYVEQRRRDKQEWLANALHEKMSPAEK
                     ELLMEALPLLTKLIDG"
     gene            4907..6307
                     /locus_tag="VGQ51_05545"
     CDS             4907..6307
                     /locus_tag="VGQ51_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9036059.1"
                     /translation="MGKRILASPVFTAFQSRNYRLYVYGQSVSLMGTWMQRTAVSWVV
                     YTLTHNTFMLGLSLFCTQFPSFLLTSIGGVVSDRYNRYKVLLTTQIASLVQSFALFII
                     VWRGNYTVWEILGLGVVLGTINAFDVPARQALVYEMVEKKENLPNALALNSSMVNLAR
                     LIGPAIAGFVLHKLGNATCFLLNTLSFLAVIGSLLRMKLAPYVRGNRDKKVLGDLKAG
                     WTYLVRHPGIGRPILMLALMSLLVIPYATLLPVYAKVIFHGNATTFGLIDSFIGMGAI
                     SCALWLAGRNVNTEIAMKKTLRINTIIFGVGLILFSHSSWFPLAMVFAVVAGFGMMAE
                     TTMTNTIIQMSVVPEMRGRVISYFAMAYFGMMPLGSLLVGFISQYVGAQNTILGQGVI
                     ALVIVALFWKHLTKSPGAQGAIPETRANGETRANGAIPAGENAATEVMAADDANEFTV
                     TSNITTNKSWKQTARL"
     gene            6283..6864
                     /locus_tag="VGQ51_05550"
     CDS             6283..6864
                     /locus_tag="VGQ51_05550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEV9036060.1"
                     /translation="METDSKALATALLVMDIQTMTMQRLGDGAETPIAAINRAIAAAR
                     AAGIPVIYVVVGFRKGFPEIGPSNKSFSVIRQAGAIPGLEDPVIVDPAVKPQPGDIVV
                     TKRRVSAFAGSDLEVILRARRIEHLVLSGFATSGVVLSTLREAADKDYRLSVLSDACA
                     DLDEEVHTVLMNKVFPRQAEVMKAAEWATGVGK"
     gene            6893..>7347
                     /locus_tag="VGQ51_05555"
     CDS             6893..>7347
                     /locus_tag="VGQ51_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV9036061.1"
                     /translation="MRIFPGSALVQLEFDKVKALLAELCNTEYAHGKAEDLRIHTEME
                     FIGLELRQTHEFKQLLQNGQYFPNDHTFNLARELKLLGIPGAILSGEQFLQVRRLAES
                     IQSIFRWFDTDRRTAWPALARVIENTRYEKAIAAAIDEVLDETGVVRDNA"
BASE COUNT         1609 a         1878 c         2217 g         1643 t
ORIGIN      
        1 atccgaaaat ggcgacggcc cgtgcgctgc ctgccatcgc atcggtgttc aggagatata
       61 atccgggcac ggtcttcgat tacacgttta acgacgccga ttatgcgaag aaattcgatt
      121 tcgagacgag gatcggtagt ctcgcgacct tttttgccgc ctttgcgatc ttcatttcgt
      181 gtttagggct tttcgggctg gcctctttca tggccgagca gcggacgaag gagatcggtg
      241 tgcgaaaggt gttgggagca tcggtgctgc atctctgggc gatgttgtct aaagattttc
      301 tgttgctggt gtcgatctct tttttggtgg ctatacccgt gtcctggtat atcatgcacg
      361 gctggttgca gcaatacgat taccggactt cgatatcggt atggatattc gtgatgacgg
      421 cggtcgcggc gcttgcgatc acgctggtca cggtgagctt tcagtccatc agggcatcgc
      481 tggcgaatcc ggtgaggagt ctgcggtcgg aataggagag gccttgcagg tggaaggtcg
      541 ccggggcatc actcggaata ggcgaggtgt tgccggtgca aggtcattcg ggcatcactc
      601 ggtgcgtaaa ctctttacgg gattggccat ggccgcacgc accgtttggt agctgacggt
      661 cagcagcgtg atgatcaggg ctcccccggc ggccgacgca aaaagccacc aggacagttc
      721 ggtccggtag gcatatttct gcagccattc atgcatcaac cagcccgaga ggggaatcgc
      781 tatggcgcag gcgatgatca ccagcatcac gaactcacgt gacaaggtat tccagagatt
      841 gaagaccgac gcgcccagta ctttccgcag ccctatctcg cgcgtgcgtt gttcggcaac
      901 gaaagaggcc agcccgaaaa gtccgagaca ggagatgaaa atggccagca ccgtaaaaaa
      961 ggtcgatact tccccgacac gttgttcgac atcgaagttc tgcgcgtaag cttcgtccgc
     1021 catttgatag gagaccaggc catcggggtc atactttctg aacacctgct gtatcctggt
     1081 catagcgtcg gcgggacgca tgttcctgtt cagccggatc gtgagataat tgaaccagct
     1141 gtaatccata aaaaagatag tgggcagggc cggatcatag ggggaatcca tgaccagatc
     1201 cttcaccacg gcgatgacgg gacgggaaat gctgtccacc gtgatccgct gaccgatggg
     1261 atcccggaag cctgcgagcc tgaccgccgc ctcgttcatg accagcccga tgctgtcgcc
     1321 ggacaggctg cgtgaaggct catgcccctg caatatctgc cagccgactg tcctgccata
     1381 gtcatgggtg atcgataccc aaccgaactg gggattggca taggggtctt ttcctttcca
     1441 ggtgagccct ccgcggaccg accaggtgtc tgtcatcggt ccgttcgacg ccgtcacgtc
     1501 ctgtgcggct ccggaagcga gcagatcgct gcggatggca ttgtatcgaa agcggagacc
     1561 ggaattgttg agcatattga cgttcagcag accttcgcgt acgtagccgg tgggcctgtt
     1621 ccgtgcatat tggatctggc gatagatgac cagtgttcct aaaatcagga aaacggatac
     1681 cgtgaactgg atcacgacca gtatctttct tggcagggcg gcgttccggc cggcacgcat
     1741 gaagcttcgg ccgagccacc ggctcggcct cgttcgggac cttgtcaggt cccctttcag
     1801 tactttcacc ggctggaacc gcgagaggta gagtgcgggg tagctcccgg agacgagtcc
     1861 cgtgaacaga gagaacccga gagcgatcag ccagaagtag ggctcacccc acggtatctt
     1921 cagatgtctg cgcgccatgc cgttgaacat aggtagaagc agcagggcca gcaacaaacc
     1981 gaatccaagc gagagcaacg atatcatcac cgattcaccc aggaattgac cgatcaattg
     2041 tccgcggagg gaaccgagcg ttttgcggat gcccacctcc cgtgcgcgac gttcgctgcg
     2101 agccgtgctg aggttcataa aattgataca ggccagcagc aggatggata gcgcgatgat
     2161 gctgaacatc cggacaaacc tgatctgtcc gcctgccggg gctccgttct tgaaattccc
     2221 ataaagatgg tacctgtcca tgggctggat gaatacggaa tccttgctgt tcgtaaggtg
     2281 gcgtctcggg atataggcga tcctttcgga gagccggtcg aagtccactc catcgttcaa
     2341 ctgaacgaag atccgggcat aataattccc ccacccgtgt tgcgaacccc tgatccaact
     2401 atgtgtttcc gcatattttt cccagggcag gaggaaatcg acaccgtgca ggtctgtgtt
     2461 ttccggtacg tcttcgtaga cgcctgcgac cttaaaggga agccgtgaat cgaggagcac
     2521 cgttcggccc atcggatcgg ccgtgccgaa aagagatcgg gccatggagg ctgtcagcag
     2581 agcggaagaa gggtcccgaa gacaatcggc cgaacccttc agcagatgga gtgtgagcat
     2641 taccggtaga tcgggctcta cccagcggca ggtccgctcg atctttttgt cggatgcctg
     2701 tcccggcgaa acgagatgcg cctcatatgg agtggccagt gcgacatgtt tgagctggtc
     2761 tccatattcc tgccgcagct gatcagccag cgggatatcg accgaatttt ccgtatgcat
     2821 cgcgccattg gaaacctgcg tgtcgtagac gatacccaac cgggaatggt tgcggtgata
     2881 atagttatag gtcacctcgt tgtacaccca cagcccgatc agcagggaca cggccatccc
     2941 ggccgccaga ccggcgatat tgaggacgct gtagaaacga tgccgggtaa gattccggaa
     3001 ggcgatgagg acataatttt tcagcatgga ggtcgtgcta gaagattatc gttctttttt
     3061 caatttattc ttaaaggctt tttcgaattt ttccaccttg gggcggatga ccacgtagca
     3121 gtagggctgt gagttgtgtg aattgtaata gttctgatga tagtcttcgg cgggatagaa
     3181 ggggccggcg ggcgtgatct cggtcacgat gggattgggg taggcgccgg ccttgtccag
     3241 ctcggcctta taatgttccg ccttttgttt ttgttcttcg gtatggtaga aaatggcgct
     3301 gcggtattgc gtgccgatat cgttgccctg gcggttcagc tgtgtcggat cgtgcgattc
     3361 gaaaaacacc tgcagcaact cgtcataaga aatgacagcc gggtcgtacg cgagctgaag
     3421 acactcggca tgcccggtgg ttccgctgca aacctcttca tagttggggt tttcgccatg
     3481 gcctcccata tagcccgagg tcacgctgac gattcctttc agttcctgga aaatggcctc
     3541 ggtacaccag aaacatccgg tgccgaaagt gacgatatcc gttgggtggg tcgcgggggg
     3601 ttgtgatgcg ctcatgttta ttaatttggg tatgtctgtg gttttagctc tcgttccggc
     3661 cgtatggaac gcaaccacca tagaacgacc aatgggaaga taaaattatg gattaccagc
     3721 aatcccagcg tttggattgt cggctgatga agaggcgctg tgtggacgaa cagctggttg
     3781 cccatgccgt tgctctgcga gaagccgaca tagggggtga aattaagcga gtagtggatg
     3841 gcgatcggta cccagatgga gccggtgcga acgaaggcgg cggcgagcac atagcccatc
     3901 aatcccgtgg tcaggaaaag gatcacgacc tgcagtgggg aatggatgcc tacgacgaaa
     3961 aaatgataga tgccgaaggc tactgccgac gtaaggaccg ctttggcgct accgatacgg
     4021 cggatcagga tgtacagcaa tgctccccgg aagatcagct cttcgaagac gacgcttttg
     4081 aacaggtacc acatggccag ccccagctgc gggagaccgt atgcctcgtt gcggtgatag
     4141 ggggcgtgtt gtatagccgc ttcacccagc tgatatgcga tcatataaat catggctagt
     4201 ccgaaaccga tgccggcgag ttgcattcgt ctggccgtcg ggcggatacc cagggcttcg
     4261 agattgcggc gttcgataaa atgtagaagg atccaggaga gcaggagttg gaccagcatg
     4321 ctatcatgga gtgaatataa aggtaaattt tgtaaagtaa ctttataagt ttaccttcgc
     4381 attatgaaaa accgacaact atccaccggt cgcgatgtac gggacggcga aatccgggag
     4441 gcaggacctc atgcgccgga cggcgaaatc ggggtcacgg gaccgacggc ccgggatatg
     4501 gaggtggcgg cagcactgcg cacaggagtg cacaggctgg tcaagctgct ccgtcgcgaa
     4561 acgcgtaatg aggcgcaact ctctcttacc gagcggtcta cactgggttt attatacccg
     4621 gatctacagc tggcgccatc ggagatcgcg cggatggaaa aggtgacgac gcagtctatg
     4681 tcacaagtaa tcaattattt aatggagctg gatctgatcg tccggacgcc ttccggggag
     4741 gacaaacgga aagtattgtt gtcgctgacc cccgccggcc gggtatatgt cgagcaacgg
     4801 cgtcgcgaca agcaggaatg gctggccaat gcgctgcatg agaagatgag cccggcggaa
     4861 aaagaattac tgatggaagc gcttccacta ttaacgaaat tgatcgatgg gtaaaaggat
     4921 attggcatca ccggtgttta cagcgtttca gagccggaac taccggttgt atgtttatgg
     4981 tcagtcggta tcgctgatgg gtacctggat gcagcggacg gccgtcagct gggtggtata
     5041 tacgctgacg cataatacct tcatgttggg tctctctttg ttttgtacgc aattcccttc
     5101 ttttctgctg acctcgatcg gcggcgtagt atcggacagg tacaatcggt ataaggtatt
     5161 actcaccaca cagatcgcct cgttggtgca gtccttcgcc ctgttcatca tcgtatggcg
     5221 ggggaattac accgtatggg agatacttgg gttaggggtg gtcctgggta cgatcaacgc
     5281 ctttgacgtc cccgcccggc aggctttggt atatgaaatg gtggagaaaa aggagaacct
     5341 gcccaatgcg cttgcattga attcttcgat ggtgaatctt gcccggctga tcgggcccgc
     5401 gatcgccgga ttcgtcttgc acaaattggg aaatgctacc tgtttcctgc tcaacacgct
     5461 gagttttctg gcggtgatcg gatcgctttt gcggatgaag ctggctcctt atgtccgtgg
     5521 caacagggac aaaaaagtgt tgggcgatct gaaagccgga tggacctatt tggtgcggca
     5581 cccggggatc ggccgaccta tcctgatgct ggcattgatg agtctgctgg tcatccccta
     5641 tgcgaccctt cttccggtgt atgcgaaggt gatcttccac ggcaatgcga ccaccttcgg
     5701 gcttatcgat agttttatcg ggatgggtgc gatcagctgt gcgttatggc tggcgggcag
     5761 gaatgtcaac acagagatag cgatgaaaaa gacgctccgc atcaatacga tcatcttcgg
     5821 cgtcggattg atcctgttct cgcattcgag ctggttcccg ctggcaatgg tctttgccgt
     5881 cgtcgccggt ttcggcatga tggccgagac caccatgacc aacaccatca tccagatgag
     5941 cgtagttccg gagatgcggg gtagggtgat cagctatttt gccatggcct atttcgggat
     6001 gatgccgttg ggtagtctgc tggtaggatt tatctcgcaa tatgtggggg cgcagaatac
     6061 gatattgggt cagggcgtca tcgcgctcgt cattgtcgcc ctgttctgga aacatttgac
     6121 gaaatcgccg ggggcgcagg gggcgatacc ggagacccgg gcaaacggag aaacccgcgc
     6181 taacggcgcg atccccgccg gggagaacgc agctacggag gtcatggccg cggatgacgc
     6241 aaacgagttc actgtcactt caaatataac aaccaacaaa tcatggaaac agacagcaag
     6301 gctttagcga ccgctctact cgtcatggat atccagacga tgaccatgca aagactggga
     6361 gatggcgccg agacaccgat cgccgccatt aatagggcta tcgcggcggc cagggcagcg
     6421 ggtatcccgg tgatctatgt cgtcgtaggg ttccgcaagg gcttcccgga gatcgggccg
     6481 tccaacaaat cgttttccgt catccgtcag gccggggcca ttccgggtct cgaagatccg
     6541 gtgatcgtcg atcccgcagt taagccgcag cccggtgata tcgtcgtcac caaacgccgg
     6601 gtgagcgcgt ttgcgggcag cgatctcgaa gtgatcctgc gggcgcgacg tatcgagcac
     6661 ctggtattga gcggctttgc gacgagcggt gtcgtcctgt cgaccctgcg cgaggcggcc
     6721 gataaggact accggttatc cgtactgtcg gatgcctgcg ccgacctgga cgaggaagtg
     6781 catacggtgt tgatgaacaa ggttttcccc cgtcaggcag aagtgatgaa ggccgctgag
     6841 tgggcgacgg gcgttggcaa ataagggcgc ttttttgtac ctttgctgcc caatgagaat
     6901 ttttcccgga tcggccctgg tgcaattgga attcgacaag gtgaaagcct tgctggcgga
     6961 gctgtgcaat accgaatacg cccacgggaa ggcggaggac ctccgtatcc atacggagat
     7021 ggaattcata gggctcgagc tgcgtcaaac gcatgagttc aaacagctgc tgcagaatgg
     7081 ccagtatttt cccaacgacc atacgttcaa tcttgcccgg gagctcaagc tgctgggtat
     7141 tcccggcgcc atcctttcgg gggaacagtt cctgcaggtg cgtcgtctcg cggaaagtat
     7201 ccaaagcatt ttccggtggt ttgacaccga ccgccggacg gcctggccgg cccttgcgcg
     7261 ggtcatcgaa aatacgcgtt atgagaaggc catcgcggcg gctatcgacg aagtgctgga
     7321 cgagacgggg gtggtacgcg acaacgc
//
LOCUS       DASYSV010000183         4268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2210163, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000183 DASYSV010000000
VERSION     DASYSV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210163"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VGQ51_05560"
     CDS             complement(<1..171)
                     /locus_tag="VGQ51_05560"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV9036062.1"
                     /translation="MSKKRVLITGAAGFLGSHLCDRFIKEGYHVIGMDNLITGDLRNI
                     EHLFKLEHFEFYH"
     gene            complement(184..1548)
                     /locus_tag="VGQ51_05565"
     CDS             complement(184..1548)
                     /locus_tag="VGQ51_05565"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEV9036063.1"
                     /translation="MKIAVIGTGYVGLVTGTCFSETGNDVVCVDIDTKKVEKLSNGQI
                     TIYEPGLEKLFLRNLKEERLRFTTQLEEGIKDAQIIFLALPTPPGEDGSADLKYILGV
                     SDHLGKLLKDYKVIVDKSTVPVGTAEKVRSAIAKNYKGEFDVVSNPEFLREGVAVDDF
                     MKPDRVVIGASSDRARRVMNDLYGPFVRQGNPIIHMDERSAELTKYAANSFLATKITF
                     MNEVALLCERLGADVDMVRRGIGSDERIGKRFLFPGIGYGGSCFPKDVQALVKSSSEV
                     DYEFRILEAVMDVNEKQKLHLLPKIKRYFKGNLKSKHFALWGLAFKPNTDDIREAPSL
                     YMIEALISEGATVTVFDPEAMNNVKQVVGDKVTYAESQYEALEGADALIIATEWNEFR
                     TPNFLKIVTSLKNKVIFDGRNLFDIAAIRELGFYYESVGRAPAIPGSLAAAANSANPA
                     SVKS"
     gene            complement(1576..2709)
                     /locus_tag="VGQ51_05570"
     CDS             complement(1576..2709)
                     /locus_tag="VGQ51_05570"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV9036064.1"
                     /translation="MQPIQMVDLKRQYTKIKSEVDAAIQEVIDNAAFINGKAVTDFVA
                     ALATYSGSKHVVPCANGTDALQIAMMALDLQPGDEVITPSFTYIATTEVVALLKLKPV
                     FAEVDPRTFCLDPASVEKAITPRTKAIVPVHLYGQAAPMREIMDIAKAHNLAVIEDNA
                     QAIGSTYRFPDGTTKRTGAIGTIGTTSFFPSKNLGCYGDGGAIFTDDDALASRLKMIA
                     NHGQSRRYYHDIVGCNSRLDSIQAAVLNVKLKRLDAYISARRAAADHYDEAFKAHPKI
                     TTPFRAAYSNHVFHQYTLVLEGVDRDGLNEFLAGKKIPSMIYYPVPAHRQQMFAASGG
                     ADHRLDTTDWLTERVISLPMHTELDDQQLQTVSAAVLEYVNKN"
     gene            2760..4157
                     /locus_tag="VGQ51_05575"
     CDS             2760..4157
                     /locus_tag="VGQ51_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEV9036065.1"
                     /translation="MRRRGSQMMSIFSYHVFLWLYKMALRIVSPWNRKARLWLDGRKG
                     LFERIAAQMATTTGAQTQAAAEVEAEAVAVSVARKKIVWVHCSSLGEFEQGRPVIEGL
                     RNGNPGCRVVLTFFSPSGYTAKKDYAGADHVFYLPLDSPRNARRFIDLIRPDLVLWVK
                     YDYWYYFLVELRRRKIPLLLVSGVFRPDQPFFRWYGRLHRYMLECFTHLFVQTEASRV
                     LLGRLRLTDQVSVSGDTRFDRVIDIAEKAEPLSLVAGFCGARPVIVAGSTWEEDEEEL
                     DHYANTNPDLRFIIAPHEISEERMREVENLFRHSIRYSALEAGRRPTVAPKGDRRHGS
                     IAPGGWPEPNVLIIDNIGMLSRLYAYATITYVGGGFGDDGVHNVLEAAVYGKPVIFGP
                     VIEKYIEAVELTDCGGGIIIDSAIEAERVFNRLLQNRQESAEAGEAARTYVYSRRGAT
                     ERIISYIQENRLLTS"
BASE COUNT          861 a         1055 c         1275 g         1077 t
ORIGIN      
        1 gtgatagaat tcgaaatgct ccagcttgaa aaggtgttcg atgttgcgga gatcgccggt
       61 gatcaggttg tccatcccga tgacgtggta gccttctttg atgaaccggt cgcagagatg
      121 cgaccctaag aaacctgcgg ccccggtgat taatacacgc tttttgctca tggtgtcggt
      181 cggttatgat tttacagatg cggggttggc ggagtttgcg gctgccgcaa gactgccggg
      241 gatggcgggg gcgcgaccga cgctttcata atagaagccc agctcgcgga tggccgctat
      301 atcgaaaagg ttacgtccgt cgaagatgac cttgttcttc agcgaagtga cgatcttcag
      361 gaagttgggg gtcctgaatt cgttccattc ggtggcgatg atgagggcat ctgctccttc
      421 gagcgcctcg tactggcttt cggcgtaggt aactttgtcg cctaccacct gcttcacatt
      481 gttcattgcc tcggggtcga atacggtgac agttgcgcct tcgctgataa gagcctcgat
      541 catatacagc gacggggcct cacggatgtc gtcggtattg ggtttgaagg ccaggcccca
      601 gagtgcaaaa tgcttgcttt tgaggttgcc cttgaaataa cgtttgatct tgggcagcag
      661 gtgcagcttt tgtttttcat tgacgtccat gacggcctcg agtatgcgga attcatagtc
      721 gacctccgac gaggatttta cgagggcttg tacgtccttg gggaaacagc tgccgccata
      781 accgatgccg gggaagagga agcgtttgcc gatacgttcg tcgctgccga tgccgcgtct
      841 gaccatatcc acgtctgcgc caagccgttc gcaaagcagg gcgacctcgt tcatgaatgt
      901 gatcttggtg gcgagaaagg aattggcagc atatttggtg agctcggcgg agcgttcgtc
      961 catatgtatg atagggttac cctggcggac gaagggaccg tagaggtcat tcatcacccg
     1021 ccgcgcgcgg tcggatgacg ctccgatgac aacgcggtcg ggtttcatga aatcgtcgac
     1081 ggcaacgcct tcgcgcagga actcggggtt ggagacgaca tcgaattctc ctttataatt
     1141 tttggcgatt gcgctccgga ccttttcggc ggtgccgacg ggcacggtgc tcttatcgac
     1201 gatgacctta tagtctttta gcaatttgcc gagatggtcc gagacgccga ggatatattt
     1261 cagatcggcc gagccgtctt ccccgggggg cgtaggcagg gcgagaaaga tgatctgtgc
     1321 atcttttata ccttcttcca gttgcgtcgt gaaccggagt ctttcttcct tgagattgcg
     1381 aaggaacagc ttttcgagac ccggctcata gatcgtgatc tggccattcg acagtttttc
     1441 gacttttttg gtatcgatat ccacgcaaac gacatcgttg ccggtttccg agaaacaggt
     1501 acccgtaacg agtccgacat aaccggtgcc gatcacagca attttcataa agatctattt
     1561 aggtttagtt tagaatcagt ttttgtttac gtattccagc accgcggcgc ttaccgtctg
     1621 caactgctgg tcatccagtt ccgtatgcat aggtaaggaa atgacccttt ccgttagcca
     1681 gtcggtcgta tccaaccggt ggtccgctcc gcccgatgcc gcgaacattt gttgtctgtg
     1741 ggcaggcacc gggtaataga tcatcgacgg gatctttttt cccgccagga attcattcaa
     1801 cccgtcgcgg tcgacgcctt cgaggaccag cgtatactga tgaaatacgt gattactgta
     1861 tgccgcccgg aacggggtcg ttatcttcgg atgagctttg aatgcctcgt catagtgatc
     1921 cgcggcggcg cgtctggcag aaatgtaggc gtccagcctt ttgagcttga cgttcaggac
     1981 ggcggcctgg atggagtcga ggcggctgtt acaacctacg atgtcgtgat aatatcgcct
     2041 gctctgtcca tggttagcaa tcattttcaa ccggctagct aacgcatcat catccgtaaa
     2101 tattgctcct ccgtccccgt aacaccccaa attcttggag gggaagaacg aggtggtccc
     2161 gatggtgccg atagcgcccg ttctttttgt cgtcccgtcg gggaagcggt aggtgcttcc
     2221 gatggcctgg gcgttgtcct cgataacggc gaggttgtgg gcttttgcga tgtccatgat
     2281 ctcccgcatg ggggccgcct gcccgtaaag atgcacgggt acgatggcct tcgttctcgg
     2341 cgtaatggcc ttttcgacgg atgcgggatc gaggcaaaag gtcctgggat cgacttctgc
     2401 gaatacgggt ttcagtttga gcaatgcgac cacttcggtg gtggcgatat aggtgaagga
     2461 gggggtgatg acctcgtcac cgggctggag atcgagggcc atcatggcta tctggagcgc
     2521 gtcggtgccg ttggcacagg ggacgacgtg tttcgaaccg gaatatgttg ccagcgcggc
     2581 cacaaaatcc gttactgcct taccattgat gaaggccgca ttatcaatta cttcctgaat
     2641 agcagcgtcc acttcgctct ttatttttgt atattgccgc ttcagatcta ccatttggat
     2701 gggctgcatg gtctttgttt tgaacgggca caaaactatt gcaaaaatgt ggtttaacaa
     2761 tgcgacggag ggggtcgcag atgatgagta ttttttcgta tcatgttttt ttatggttgt
     2821 ataagatggc cttgcggata gtttcgccct ggaacaggaa ggcccggctt tggctggatg
     2881 ggagaaaggg gcttttcgaa aggatagccg cgcagatggc gaccactacc ggtgcgcaaa
     2941 cgcaggctgc ggcggaggtg gaagcggagg ctgtggcggt ctccgtcgcc cgaaaaaaaa
     3001 tcgtttgggt gcattgttct tccctcggcg agttcgagca gggccggccc gtgatagaag
     3061 gcctgcgaaa cggcaatccc ggatgtcgtg tcgttctgac gtttttttct ccttcggggt
     3121 acactgccaa aaaagattat gcgggtgcgg accatgtatt ctatctgccg ctggacagtc
     3181 cgcgcaacgc ccgtcgtttc atcgatctta tccgtcccga tctcgtacta tgggtcaaat
     3241 acgattactg gtattatttc ctggtggagc tgcggcggcg aaagataccg ctgttactgg
     3301 tttccggcgt tttccggccc gaccagccgt tcttccggtg gtatggcagg ttgcatcgtt
     3361 acatgctcga atgtttcacg catctcttcg tgcagaccga agcctcgcgt gtcttgctgg
     3421 ggcggcttcg attgacggac caggtgagtg tcagtggcga cacgcggttc gaccgggtca
     3481 tagacatcgc cgaaaaggcc gagccgcttt cgctggtagc cgggttctgc ggcgcgcggc
     3541 cggtgatcgt cgcgggcagc acctgggaag aagatgaaga agagctggat cattatgcca
     3601 atacgaatcc cgatctgcgg tttattatcg ctccgcatga gatcagcgag gagcggatgc
     3661 gggaggtcga aaacctgttc cggcattcga tccggtattc ggccctggaa gcgggacgtc
     3721 gccccaccgt cgcgccgaaa ggggatcgga ggcatggttc gatcgcgccg ggagggtggc
     3781 cggagcccaa tgtcttgatc atcgacaata tcggcatgct atcgcgtctc tacgcatatg
     3841 cgaccatcac ctatgtcgga ggagggttcg gggatgatgg agtacacaat gttctggagg
     3901 cagccgtcta tggcaaacca gtaattttcg gacctgtgat cgaaaaatat atcgaggcgg
     3961 tggagctgac ggattgcggc ggcgggatca ttatcgatag cgccatcgag gcggaacggg
     4021 tgttcaatcg tcttttacag aaccggcagg aaagtgcgga agccggagag gcggctcgca
     4081 cgtatgtgta ttcgcggcgc ggagctaccg agcggataat atcgtatatt caggagaacc
     4141 gtctcttgac cagctgatag aaatgtcgcg tggtcgggtt attctcatcc caaccgagct
     4201 tcactttcag ttctctttcc atccagtatt cggcttcggg gagtattctg aatccattga
     4261 tcgtcttg
//
LOCUS       DASYSV010000184         5912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_417297, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000184 DASYSV010000000
VERSION     DASYSV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417297"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3017
                     /locus_tag="VGQ51_05580"
     CDS             <1..3017
                     /locus_tag="VGQ51_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9036066.1"
                     /translation="NYALRIPVMEKAVLVFTYVGYSTQEVKVYGRSVIDVVLEKSNSS
                     LDDVVVVGYQTMHRRDLTGAVSSVNSKQIKDFPLSSAAQALEGRLAGVNISSTEGAPG
                     ADVTVTIRGGGSITQDNSPIYIVDGIQVENALSTISPQDIASVDVLKDAASTAIYGAR
                     GANGVVIITTKSGIAGRTRVSYNASFGFDELTKHIPVLSPYDFVVWQYERYKLTKLTD
                     SSFFHEYGSTWDTLSVYKKSTPLDWQDQVFRKAGFQNHNVVVSGGNQNTTFNLSLTDN
                     IENGLMINSGLDRKLVNFRLDHKADRLRVGIAVRYMDQVVTGNGTSSISGTSNGLRNS
                     VQYRPLAIPTEPDPNSFDPSYYAAANQITNPVLSAQQEYKRVPTTGTNISGYLSYALL
                     KNLTFRTTVGFDNTSITQDQFHGTVTYTAHNYGSMPVASIGYQHNTTINNSNTLEYSK
                     TGREGLNTWHVLLGQEIYQVNANNTYMETRYFPSNISPDQALANMGLGSPPSGSTEPL
                     PTSGVNPPNRTLSFFGRINYEYNNEFLASAALRSDRSSKFNSDNGLMLFPSATFGWRL
                     SHEKFMENLPFINDAKLRFGYGVAGNNRIGDLLYEQLYGVTGQYAIDRTVLPGFAPSA
                     LANPGLKWERTASRNLGLDLSLLNNKVQFTVDAYYNTSDDLLLQVAIPPTTGYTSQVE
                     NVGGTSNRGLEFQIASTLIQKRDFSWSSNFNISFNRNRVENLGGLKQQTRSSGYQGAD
                     GADDFLVRVGSPVGLMYGMVTDGFYKVSDFDYNSSTGAYTLKKGVTDDSQIYGTAQPG
                     QIKLKDINGDGIITLDSDRTVIGNANPKFFGGWNNQFAYKNFDLSIFVNFVYGNKIDN
                     ANKTQWTDGSFQDVNMLATMKNRWTNIDAQGNLVTDPNELEQMNVHAKIWSPVRNQRY
                     YLTSWAVEDGSYLRINNLTLGYSLPKSILQRAKISGVRIYGTVNNLYTITGYSGYDPE
                     VNTRRSDPLTPGVDLAGYPRGRTWLLGANINF"
     gene            3037..4851
                     /locus_tag="VGQ51_05585"
     CDS             3037..4851
                     /locus_tag="VGQ51_05585"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9036067.1"
                     /translation="MKPKFNMLVVLTTVFLSLVTFQCKKFTQVSPSSQFSLPQVFSNV
                     NNAYTAVIGVYDQLMGDGTYGDIMSQYFPYDCDEMITTGNIDNGRRGMARYQLLLSNT
                     VLRSPFTSLYSGVEKANLCIEQIPKMDMYIHGDTSDKAQLHRLYGESLTLRAQFLFEL
                     VRNWGDVPAPLVPSYQDKNLFLPRADRDSTYDHLIADLKTAEDLVPWRTEVPRDSRIT
                     KGAVKALRARIALFRGGYSLRSDTRTMQRRSDYLTYYQIARDECADLMARRDEHTLDP
                     VFGDIWHKYLCGFQYDPWGEILFEVGAGGGNSNSDSKMGNYGSTVTATNSRYGSGIGG
                     IAVNPQYYYDFDSLDTRRDITVSWYSVNADNTRTFRTLTSLTPAKFGRDFRNPLLPGS
                     VQNCGYNWPLIRFSDVLLMFAEADNELNQGPNAADIAAFEEVRKRGFKGNENMIGVTP
                     TDYAGFFNAIVNERLLEFGGEGIRKYDLLRWNLLGAKIQEARDEIQQMSTGGGPYANI
                     PRFIFWKQSGETILFYGGANGGTVPPLYTPGVTTTPSGYTKTYWRDQLTLSSSHIDGL
                     PLWQAIAEDFTPGKSELFPIDQATLDSYNGKLQQNPGY"
     gene            4878..>5912
                     /locus_tag="VGQ51_05590"
     CDS             4878..>5912
                     /locus_tag="VGQ51_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9036068.1"
                     /translation="MMQAIGKYRWVIVTLLFFATTINYLDRQVIGLLKDALARDLHWS
                     EKDYGRIVTAFSAAYAIGLLLLGRITDKIGTKVGYSIAISIWSLAAIAHAFVGTTFGF
                     GAVRSTLGLGEGGNFPAAIKVVAEWFPKKERAFATGIFNSGSNIAAVIGPFLVMWIYE
                     SYGWRAAFIWTGAIGFVWLIFWWIFYAVPARQWRLGKAELEYINSDAGEGSDATGLGR
                     VLTLRQTWALIAGRFFTDPIWWFYLFWIPSWLNTTFQMDLQHSWIYVSVIYVVASLGS
                     ILGGYLSGWLIKKGWAVYKARKTAMLLCALCVVPVIFIRWTTGVWPAVGLISLAAAAH
                     QAWGANIFTVV"
BASE COUNT         1397 a         1520 c         1644 g         1351 t
ORIGIN      
        1 gcaactatgc gctccgtatt ccggtgatgg aaaaggccgt gctggtgttt acctatgtgg
       61 gctattctac ccaagaggta aaagtctatg gcaggtctgt cattgacgtc gtactggaaa
      121 aaagcaactc ttcgctcgat gacgtcgtgg tcgtcggtta tcagacgatg catcgaaggg
      181 acctgacagg cgcagtgtct tcggtaaatt ccaaacaaat aaaggacttc cccctgtcgt
      241 ccgccgcgca ggcgttggaa ggacggctgg cgggtgtgaa catcagcagc acggaaggcg
      301 caccaggggc ggacgtgacc gtcacgatac gcggcggcgg ctccatcacc caggacaact
      361 cacctatata tatagtggac ggcatccagg tagagaatgc gttgtcgacg atctcgcctc
      421 aggacattgc ctcggtagac gtcctgaagg atgcggcttc caccgcgatc tatggcgcca
      481 ggggcgccaa cggggtagtg atcattacca ccaagtccgg gattgcgggc aggacgaggg
      541 tcagctataa cgcgtccttc ggattcgacg agttgacgaa gcacataccc gtactcagtc
      601 cttatgattt cgtggtctgg caatatgagc ggtacaagct cacgaagctt acagacagtt
      661 cttttttcca cgaatacggc agcacctggg acaccctgag cgtatataag aaatcaaccc
      721 cgctggattg gcaggaccag gtcttccgga aagccggttt ccagaatcat aatgtcgtgg
      781 tcagcggagg gaatcaaaac acgactttta atctcagtct caccgataat atcgagaacg
      841 gcctgatgat caattccggc ctggatcgga aactggttaa tttcaggctc gaccacaagg
      901 cagaccggct cagggtcggc atcgcggtgc gttatatgga ccaggtggtc accggcaatg
      961 ggaccagctc catttcggga acttcgaatg gactccgcaa ctccgtccaa taccggccgc
     1021 tggcgatccc caccgaaccg gaccccaaca gcttcgaccc ctcttattac gcggcggcca
     1081 accagatcac gaatcccgta ctatctgcac agcaggagta taaaagggtg ccgaccaccg
     1141 ggaccaacat cagcggctac ctgagttacg cactgctgaa gaacctgacc ttccggacca
     1201 ccgtcgggtt cgataatacc agcattacgc aggaccagtt ccatggaacg gtgacctata
     1261 ccgcccacaa ctatggcagc atgccggtgg cttctatcgg ctatcagcac aataccacca
     1321 tcaacaacag caatacgctg gaatatagca agacgggcag ggaaggtctc aatacctggc
     1381 atgtcttgct gggtcaggag atctaccagg tcaacgccaa caacacttat atggagaccc
     1441 ggtatttccc gtcaaatatc tctcccgacc aggctttggc gaacatgggt ctgggttcgc
     1501 cgccatccgg ctctaccgag ccgcttccta ccagcggggt aaatcctccc aatcgcacgt
     1561 tgtcattttt tggcaggatc aactatgaat ataacaacga attcctcgcg agtgccgctt
     1621 tgcgatcgga caggtcttcc aaattcaata gtgacaacgg gctgatgctc ttcccttcgg
     1681 ccacattcgg gtggagattg tcgcatgaga aattcatgga aaacttaccc ttcatcaatg
     1741 acgccaagct gcgtttcgga tatggtgtcg ccggcaacaa ccgtatcggc gacctgctat
     1801 atgaacagct gtacggcgtc accggtcagt atgcgatcga ccgtacggtg ctacccggct
     1861 ttgctccctc cgcgctggcg aaccccggtc tgaaatggga gaggactgct tcccgcaatc
     1921 tcgggctcga tctctcgctg ctgaacaaca aggtgcaatt taccgtggat gcctattaca
     1981 atacgagtga tgacctcctg ttgcaggtgg cgatccctcc caccacggga tacacctcac
     2041 aggtggagaa tgtaggggga acttcgaacc gtggtttgga attccagatc gccagtacgc
     2101 ttatccaaaa gcgggatttt agctggtcgt ccaatttcaa tatctcgttc aatcgcaaca
     2161 gggtcgagaa cctgggagga ttgaaacagc aaacccgcag ttcgggttac cagggggccg
     2221 acggcgccga tgattttctc gtaagggttg gcagcccggt tggtctgatg tatggcatgg
     2281 tcacggatgg attctataag gtgtcggatt tcgattataa ttcgtcaacc ggagcgtata
     2341 ccctgaaaaa aggggtaacg gatgactcgc agatctacgg cacggcacag ccagggcaga
     2401 tcaaattgaa ggacatcaac ggcgatggca tcattacgct ggacagcgac cggacggtca
     2461 tcgggaacgc gaatccgaaa ttcttcggag ggtggaacaa ccaattcgcg tacaagaatt
     2521 tcgacctcag cattttcgtg aatttcgtct atggcaataa gatcgacaac gccaataaga
     2581 cccagtggac ggatggcagc ttccaggacg tgaacatgct ggcgaccatg aagaaccgtt
     2641 ggacgaatat cgacgcccag ggcaacctgg tgacggatcc gaacgaactc gagcaaatga
     2701 acgtgcatgc gaagatatgg tcccccgtcc gcaatcagcg ctattatctc acctcctggg
     2761 ctgtcgaaga tggctcttac ctccgcatca ataacctgac attgggatat tctcttccga
     2821 aatcgatcct gcaaagggca aagatatccg gcgtccgcat ctacggcacg gtgaacaacc
     2881 tgtatacgat cacgggctat tccggatacg atcctgaagt gaacacacgg cggtccgacc
     2941 cgctgacgcc gggtgtcgat ctggccggtt acccgagagg caggacctgg ctgctgggag
     3001 caaacatcaa tttctaaaca tcatcaaatc atttttatga agccgaagtt caatatgctc
     3061 gtggtcctga cgacggtatt cctgtcgctg gtcacctttc aatgtaaaaa gttcactcag
     3121 gtgagcccca gctcgcagtt cagcctgccg caggtatttt ccaatgtcaa caatgcctac
     3181 accgcggtga taggggtata tgaccagctg atgggcgatg gcacctatgg cgacatcatg
     3241 agccagtatt tcccgtatga ttgcgatgaa atgatcacga cgggcaacat cgataacggt
     3301 cgtcgcggta tggcacgcta tcaattgctg ctgtccaata cggtactgag gagcccgttc
     3361 accagtcttt attcaggcgt cgaaaaagcc aatctctgta tcgagcagat accgaaaatg
     3421 gatatgtata ttcacggaga cacgagcgac aaggcgcaat tgcatcggct gtatggcgaa
     3481 tcgcttacgc tgagggcgca attccttttc gaattggtcc gcaactgggg agacgtaccg
     3541 gcgccgctcg taccttccta ccaggataaa aacctgtttc ttccgagggc cgatcgggat
     3601 tctacctatg accatctcat cgcggatctg aagacggctg aagacctcgt gccgtggcgg
     3661 accgaggtgc ccagggactc gaggatcacg aaaggcgcgg taaaggctct ccgtgcgagg
     3721 atcgcattat tccggggagg atattctttg cgtagcgaca ccaggacgat gcagcgtcgc
     3781 tcggattatc tcacctatta ccagatagcg cgggatgaat gtgccgatct catggcacgc
     3841 agggatgagc ataccctgga tccggtgttc ggtgatatct ggcataaata cctgtgtggt
     3901 ttccaatacg acccctgggg cgagatcctg ttcgaagtag gggcaggggg cggcaatagc
     3961 aactcggact ccaagatggg caactatggc agcaccgtta cggccacgaa ctcccgttat
     4021 ggatccggga tcgggggcat cgcggtcaat ccccaatatt attacgactt cgattcgctg
     4081 gatacgcgca gggacattac ggtcagctgg tactcggtaa atgccgacaa tacgcgtact
     4141 ttcaggacgc tgacatcgct gacacctgcc aaattcggca gggactttcg taatcccctg
     4201 ttaccgggat cggtacagaa ttgcggatat aactggcctc tcatccgatt ttccgacgtg
     4261 ttgctgatgt ttgccgaggc ggataacgaa ttgaaccagg gacccaatgc tgccgatatc
     4321 gctgccttcg aagaagtgcg caaacgtggg ttcaaaggca acgagaatat gatcggggtc
     4381 acacctacgg attatgcagg gttcttcaat gccatcgtga acgagcggct cctggaattc
     4441 ggcggtgaag gcattcgtaa atatgacctt ctccggtgga acctgcttgg cgccaagata
     4501 caggaggcca gggacgagat ccagcagatg tctaccggcg gcggtccata tgccaatatt
     4561 cccaggttca tcttctggaa acaaagcggc gaaacgatcc tgttttatgg aggggccaac
     4621 gggggaactg ttccgccgtt gtatacgccg ggtgttacca ctacgccatc gggatatacg
     4681 aaaacgtatt ggcgcgatca gcttacactt tccagcagcc atatcgacgg attgccgctg
     4741 tggcaagcaa ttgcagagga ttttacgccc gggaagagcg agttgttccc aatcgatcag
     4801 gcgacattgg attcttataa tgggaagctt cagcagaatc cgggttactg attttttccc
     4861 gtatttttaa accgacgatg atgcaagcca ttgggaaata cagatgggtg atcgttaccc
     4921 tattattttt tgcgacaacc atcaattatc tggacagaca ggtcatcggt cttttgaagg
     4981 atgccctggc acgggacctc cactggtcgg aaaaggacta tgggcggatc gtgaccgctt
     5041 tttccgcggc gtatgccata gggttgttgc ttctcggaag gattaccgat aagatcggga
     5101 cgaaggtggg ttattcgatc gccatttcca tctggagcct ggcagcgatc gcccatgcct
     5161 ttgtcgggac cacgtttggt ttcggcgccg tacgcagcac acttggtttg ggagaaggag
     5221 gaaattttcc ggcagcaatc aaagtggttg ccgaatggtt ccctaaaaaa gagagggcgt
     5281 ttgctaccgg tattttcaat agcggttcga atatagcagc tgtcatcggt ccgttcctgg
     5341 tcatgtggat atacgagagc tatggttggc gtgcggcttt tatctggacg ggtgcgatag
     5401 gttttgtgtg gctgatcttc tggtggatat tttatgcggt gcccgcgagg cagtggcggc
     5461 tgggcaaggc cgaattggaa tatattaaca gtgatgccgg tgagggaagc gacgcgacgg
     5521 gtttgggaag ggttttgacg ctcagacaga cctgggcgct gatagcgggc cggttcttca
     5581 cggatccgat ctggtggttc tatctttttt ggatcccctc ctggttgaat actacttttc
     5641 aaatggacct gcagcattcg tggatctatg tatccgtgat ctatgtcgta gcgagtctgg
     5701 gcagcatcct ggggggctat ctgtcaggat ggctgatcaa aaagggatgg gcggtctaca
     5761 aggctcgcaa aacggccatg ttgttgtgtg ctttgtgtgt ggtccccgtc atctttatac
     5821 gttggaccac gggagtctgg ccggctgtgg gtcttatcag cctggctgcc gccgctcacc
     5881 aggcctgggg agcgaatata tttaccgtgg tg
//
LOCUS       DASYSV010000185        10863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1314334, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000185 DASYSV010000000
VERSION     DASYSV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10863
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314334"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VGQ51_05595"
     CDS             complement(<1..895)
                     /locus_tag="VGQ51_05595"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HEV9036069.1"
                     /translation="MQQTKLLSVSILLAACLLFACSCNNRDNSAKKDNPSHASANNSD
                     STLSQLKGLSSMFGKADSGILKNLGGGGGMDSMMNKMQAMMGGKKGNPGDAVANSVLN
                     LQLGQMKDNNPLKSVAKGMMEAQNNGTAGPSKTYTAAYTPEQSEDYQVPVSGNGNAIM
                     LQYTGGAVVNNKKDGLWKNVYISTTGANKWNVYAEGYAESSALNMKVHSTLLANINET
                     YSIILNDQYKKYSKQQRSDLGKNESGVQVQKIGTERLFGYKCVHIKVTYTIKALGETA
                     HEENDEWYSTEVPGSQFLSSLV"
     gene            complement(924..1502)
                     /locus_tag="VGQ51_05600"
     CDS             complement(924..1502)
                     /locus_tag="VGQ51_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036070.1"
                     /translation="MERANTTPILLFAGLLFFCACGNSSGNKADASDAKAGSSTVQAT
                     SPDINLTGRNGRFSYTINGERVETINYVQHANLFINEVSNDAANGMVKIAVTCSGNNV
                     FDFSIANSGTTTIDNHQPSPSAVTGKKEASYMDGKTSRNLYAVSLTVTITSMDNSRIS
                     GTFSGTFKADESDGGATANITSGSFDLPFLKN"
     gene            complement(1641..2873)
                     /locus_tag="VGQ51_05605"
     CDS             complement(1641..2873)
                     /locus_tag="VGQ51_05605"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEV9036071.1"
                     /translation="MKMQGQPTFYEVGPAAIGLLLCAGVFMLSCNPAPAKRYGFLTML
                     GHDTIAIEDITRQGNTLTSDDVDRFPRVRIRHTVVDLDEDGSIRHLEMEIHTPSEPSG
                     QRDRKVVADVAGNKVHLSKTDSTGTVTRDFATGGSIVVAHVPQMYSLYELYFAAAMKK
                     SAASKLPAGSHVPMRQFYIDREFDRFPLGEATVTPLGNGKVEVTHDWLSGTGEAIMDS
                     GDNMLSYSGARTTYKVDVRQLASPPGVQSIASRWEEKEGGNVKSLSVRDSIGAQIGNA
                     VLTVEYSRPLLRGRTLLGDVIPYDRVWRTGANAATQFRTSAPIKLGGMQVPAGTYTLF
                     TAPHSSGVDLIVNKQTGEWGTEYNGSLDLGRVRMTSEDATAPVEEFTISIVPVDSHHG
                     RLVFEWGSFRWISPVEVQ"
     gene            3237..3923
                     /locus_tag="VGQ51_05610"
     CDS             3237..3923
                     /locus_tag="VGQ51_05610"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEV9036072.1"
                     /translation="MGWPFPGRKDRSDTVSGRILERRASHILVSDHPDGPYTPMADST
                     YLPANKSTLDGTFWVDKDGKPYLVYCHEWLQNGDGTIEKIELKSDLSGSIGTGSLLFR
                     ASSSPWSREKDANGTVRPNKVTDGPYVFRTQTGRLGMLWTSWVYDVYTQGVAYSATGT
                     LEGPWIQEKEPITPPNFGHGMLFRTLQGKLLMAIHSHQNINGHYRRVPHLFEVDDSGD
                     KLIVRGPYHP"
     gene            4022..4465
                     /locus_tag="VGQ51_05615"
     CDS             4022..4465
                     /locus_tag="VGQ51_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEV9036073.1"
                     /translation="MLNTILSNLYEKDLRVLIEEVKQFKVEDNLWQTTGSVKNSAGNL
                     ALHIIGGLNHFFGATLANTGYIRTRDQEFSKKGVKREDLITGLEEVIGIVTNTLNNLT
                     QKELEAEYPLIFDGEKKTTMFIVVRMYAHLSYHLGQINYLRRILE"
     gene            4471..6285
                     /locus_tag="VGQ51_05620"
     CDS             4471..6285
                     /locus_tag="VGQ51_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEV9036074.1"
                     /translation="MKKIFTAICLWSCHTLLAQSEKGAIDLSQLQTPQPHYLPEYTFD
                     SSTDPARWQAEKHGLRVSFASTDIHYFRTEVPDIEQTTEFRAAGWKADRVNALILVWS
                     PDTLEQVRFVVHDLVNTRGQRLDKKNIHLNMVRYVVSNYPYNAKDATCDVSPYKKAFL
                     MPDRFETFEQFDVPGRTVRPVWLSLDIPAGSLPGIYSTTIEAKCKGYSVPLKISIKVQ
                     NEQLPPPHEWKYRLDLWQNPWVVAWYNHVKPWSPEHKMLLKKHLALYASAGGKYITTY
                     AVHSPWSDNSYMIEETMIEWIKRKSGSWQFDYHIFDEYVQLAMEAGIDKAITIYTPVP
                     WGDRFRYMDERTGDYVYELWTPGSDAFKSHWNAFLNDLKKHLQQKGWLNKTYLGINET
                     TLDETMAAIRAIRANSPDWKITYAGNWHKELDGLLNDYCCLYGNEPGVDDVKARAAKG
                     FTSTYYICCNPPKPNDFIFSPPVEGRWLGWYAAAHGYDGFLRWAYDAWPADPARDARH
                     AIWPAGDCFLVYPGGNSCIRFERLREGIVDYEKIDILRKKARASTDPKIKDLLKQLDD
                     HLQTLLAEHEFRTENVISEVEKGKKIIEELSDLEGGRS"
     gene            6282..8078
                     /locus_tag="VGQ51_05625"
     CDS             6282..8078
                     /locus_tag="VGQ51_05625"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HEV9036075.1"
                     /translation="MRTGSIILAIIITLTARTLKAADSLSCTEYDDYILTHQLTPFGP
                     VPTALDPNGVYPYVSYCETSNRPALKKYHFIVLQNDDIKITVCPDLGGKIFSMIHKPS
                     GKEVLYVPGIIRQTRILPRFYFVAGGIEVSFPISHTPVQNEKLLYRIDRTPDRIYVTC
                     GERELRFGMQWSVEYSLGMNDPFLTERVVFRNPGSLAYPWMSWSNAAIPAAPDTRFDF
                     PKGRVLSHASTVDTIDWEKQGPRTQSDIKEMTGYFWESKDVNAFGAFTKTLGIGLYHI
                     ADAHITGGIKLWSYGVGEDSAWSVLSTASHQTYLELQGGPLSDQSVKAELQPKETKWH
                     LEYWIPSDHPLDIYSLKLPNTRLRPVTSIPLFEWARPKEVKIWKDLAGAYNKRSIPPT
                     PPAIDEYCWAPSGMEDLDGAFQWAIRNGKKEQQQLWWFYYGTWLAGRGMTDSAIQALS
                     TSDMGIAKALLARLYHLKGNNNAAADAIRSIQEPWLQRHPQIVVERDKYLRALGPQTL
                     HERDQSLATVDALKDEWVIERKVQLLIDKGEFQKAKDILLSTPFQKVHQTYTRTGLWK
                     QITDKLNIPFLPIPVSLGEDRLAHFGAYREYE"
     gene            complement(8014..9069)
                     /locus_tag="VGQ51_05630"
     CDS             complement(8014..9069)
                     /locus_tag="VGQ51_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEV9036076.1"
                     /translation="MSKILATLYQELIGFLGIGPLLDMYHKGDYSSLHTLQGIENAIG
                     PVIPFLLLLEIVRAAFFRRFKVIDYKVQFFTYVFNQFAGRFIAIAATAYCIALFQKHG
                     LLRTTFTWYWLIYGYIAWELSHFVYHYLAHKVRLLWCLHSTHHAPQTMNLSVTYAHFI
                     LEAPYADVIRTSICILLGVNPPLLFFIMFIDGTWGAFIHAGENVLKDGKLGFLNKIIL
                     TPSRHRVHHARNPVYMDTNFCNLLNIWDRVFGTLADERRDIPIEYGITRKMNPNNFWD
                     VYFGEIVALSKDVYAAPGLKNKFLYIVMPPGWSHTGDHKTAKVVRKEFLQQAQPSIHI
                     PDMPRNEPACLPRETQG"
     gene            9142..10047
                     /locus_tag="VGQ51_05635"
     CDS             9142..10047
                     /locus_tag="VGQ51_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036077.1"
                     /translation="MTRASQRQVWLLGTLAISAFLVATIGCLDAGPDGPTQKHVQYYA
                     MLGTRGIWQDGWKASAVHAAITGKGNFDKDQWQLYHVDADRSESQDSPQQYPDKLKAL
                     VDLWFEEAKKNNVLPLDDRTAAEQLGVDRPSQGPPRDRYIYYPGTEAVPEGVAVNIRN
                     RSYKILANVELGPDASGVIFAHGSRFGGHSLFIKDHKVFYVYNFLGIRPEQVFSSPPL
                     PPGRYTLGVGFTRDSTGKNGESVGHVKLYVNDKIVAGGLMRTQLGKFTLSGDGLCVGF
                     DSGDAVSDQYKTPGTFQGGVINLSA"
     gene            10124..>10863
                     /locus_tag="VGQ51_05640"
     CDS             10124..>10863
                     /locus_tag="VGQ51_05640"
                     /inference="COORDINATES: protein motif:HMM:NF023521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HEV9036078.1"
                     /translation="MRTNKKILAALAILFALGCPAMAQDSTATHQRTYKNVIRYDLSG
                     GLLFGFGKYVVFGYERVLGPHQSMSVNIGPASLPRLLSIETDSFSVSKDTKRKGFNVS
                     VDYRFYLAKENKYGPPRGLYIGPYISYNQFERNNEWNYNSATSPQQVVHTTTEFKILT
                     LGGEIGYQFVIWKRLAIDMVMIGPGFSHYDLHSVIHNTLSERAKEQIQSAIKQLITQR
                     FPGMNYVFSDKQFDANGRIRTWDVGYRY"
BASE COUNT         2788 a         2781 c         2658 g         2636 t
ORIGIN      
        1 aaaccaggga ggataaaaat tgagaacctg gtacttcagt gctgtaccac tcgtcatttt
       61 cctcgtgtgc cgtttctccc aacgctttta tcgtgtaagt aactttgata tgcacacatt
      121 tgtaaccaaa cagccgctca gtcccgattt tttgcacctg aacgcctgat tcgtttttcc
      181 ccaggtcgct tctttgctgc ttgctatatt ttttatactg atcattcagg atgatggaat
      241 aggtttcgtt tatatttgcc agaagggtcg aatgcacttt catatttaag gcggatgatt
      301 ctgcatagcc ttcagcatac acattccact tatttgctcc ggtggtgctg atgtatacgt
      361 ttttccagag cccatctttt ttattgttga cgacagcgcc ccccgtatat tgaagcatga
      421 tggcatttcc attgcccgaa acaggtactt gataatcctc tgattgctca ggagtatagg
      481 cggcggtata tgttttggaa ggcccggcgg tgccattatt ttgagcctcc atcattcctt
      541 tggcaacgga ttttaatgga ttgttgtcct tcatttgtcc cagctgcaga ttcagtaccg
      601 aattggcaac tgcatcgccg gggttccctt ttttaccgcc catcattgcc tgcattttgt
      661 tcatcatact atccatcccg cctccgccgc caaggttttt taggatgcct gaatcggctt
      721 taccaaacat tgagctaagg cccttcaatt gcgaaagcgt gctatcggaa ttgttggcag
      781 atgcgtgtga tggattatct ttcttagcac tattatcccg gttgttgcat gaacaggcga
      841 atagcagaca cgctgcaaga agtatactga ctgataacaa ctttgtttgt tgcatataat
      901 cgggatgttg ggctattgac atttcaattc tttaggaaag gcagatcaaa actgccggag
      961 gtgatgtttg ccgttgcccc gccgtcgctt tcatccgcct tgaatgtacc ggaaaacgtt
     1021 cctgagatac ggctgttgtc catgctagta atcgttactg tcaatgaaac ggcgtaaagg
     1081 tttctggatg ttttgccatc catgtaggaa gcctctttct tacctgtaac agcggaaggt
     1141 gacggttgat gattgtctat ggtagtagtt ccgctattgg cgatggaaaa gtcgaatacg
     1201 ttgtttccgg agcacgtaac cgctatcttt accatcccat tggccgcatc gttacttacc
     1261 tcatttatga ataagttggc gtgttgcaca tagtttatcg tctcaacccg ttctccgttt
     1321 attgtataag aaaagcgccc atttcttccg gtgagattta tatcagggga tgtcgcttgg
     1381 acagtcgacg aacctgcttt ggcatctgat gcatcagctt tatttcctga tgaattgccg
     1441 catgcgcaga aaaatagtaa gcccgcgaag agaagaatag gagttgtatt tgcccgttcc
     1501 ataaaacttg tttgtttggt agattaatgg gtgcccggtc aagtacttaa agttgcactt
     1561 cccctattcg gttttgataa ataaccgaca agtggcatgt taaaatcgac agaaagaaga
     1621 gaggagtgtt caatcccatc tcattgtact tcgaccggcg aaatccaccg gaacgacccc
     1681 cattcgaata cgagcctacc atggtgacta tcgacgggta cgatggatat cgtgaactct
     1741 tccacggggg cggtcgcatc ttcactggtc atcctcaccc tgccaaggtc gagcgagcca
     1801 ttgtattctg tgccccattc accggtctgc ttgttcacga tcaggtcaac gccgcttgag
     1861 tgcggagcgg taaacagcgt gtaagtaccc gcgggaacct gcatcccgcc gagtttgatg
     1921 ggggccgacg tcctgaactg tgtagccgca ttggcgcctg ttctccatac acggtcgtaa
     1981 ggtataacat cccccagcag ggtccgtcca cgcaacagcg gcctgctata ttcgacagtg
     2041 agaactgcat tgccgatttg cgccccaatc gaatcccgaa cgctcagcga cttcacgttg
     2101 ccgccttctt tctcctccca cctgctggcg atgctttgaa cgccgggcgg actcgcgagt
     2161 tgtcttacat ctactttgta tgtagtgcgt gcgcccgaat agctgagcat attgtcaccg
     2221 gagtccataa ttgcctcgcc ggttccggaa agccagtcat gcgtaacctc gaccttgccg
     2281 ttcccgagag gcgttaccgt ggcttcgccc aaaggaaacc ggtcgaattc ccggtcgata
     2341 taaaactgtc tcataggcac gtggctaccc gcgggcagct tggatgccgc cgatttcttc
     2401 attgccgctg cgaagtacag ctcatagaga ctatacatct gcggtacatg cgccaccaca
     2461 atactaccac cggtcgcgaa atcacgagtc accgtaccgg tgctgtctgt ttttgaaaga
     2521 tgtactttgt ttcccgcgac atcggcaact acttttcggt cccgttgacc ggatggttca
     2581 ctgggtgtat gaatttccat ttcaaggtgc cgtatgctac cgtcttcgtc aagatcaacg
     2641 acggtatgcc gtatccgcac ccgtggaaag cgatccacat catcgcttgt taaagtattg
     2701 ccctgccgtg tgatatcctc tatcgcaatc gtatcatgcc caagcatcgt aagaaaaccg
     2761 tatcgttttg ccggcgcagg attgcaggaa agcataaaaa cccctgcaca aagaagaagg
     2821 ccgatcgcag ccggcccgac ctcataaaaa gttggttgtc cttgcatttt cattccattc
     2881 attgttaaac taccgaattc tttcgtattt ttcggaacca aatgaactgt cgtaccgcag
     2941 tttatttgaa aggtttattc aaaggctgga aggcgaaatg gcaatagctt gtgccgcagg
     3001 aatgggacag atggaacgcc gacagacttc taaaatatgt tcattaaagc tctatatttc
     3061 atcttaattc ttccggtgcc tttattcgct caggaaagaa cgatcgtctt taaagaaaat
     3121 atacccctgg attccatccg gcttagcgac ccttgtatcc tggcagatgc taaaacttcg
     3181 atgtactaca tgaccgggac ggggggcatg atatggaaaa gcaaagatct tagattttgg
     3241 gatggccctt tccaggtcgt aaagaccgat ccgatacggt gagcggacgg atattggaac
     3301 gtcgcgcgtc tcacatcctg gtgagtgatc atccggacgg tccatatacg ccgatggcgg
     3361 attcgaccta tttgcctgcc aataaatcga ctttggatgg gaccttttgg gtggataagg
     3421 acggaaaacc gtacctggtc tattgccatg aatggctgca gaacggggat gggactatcg
     3481 aaaagattga gctgaaatcc gatttgagcg gatcgatcgg aacaggaagc ctgttattcc
     3541 gcgccagtag ttcgccttgg agccgcgaaa aggacgcgaa tgggactgtc cgaccaaata
     3601 aagtaaccga cgggccgtat gtattccgca ctcaaaccgg gcggctgggt atgctctgga
     3661 cgagttgggt atatgacgtt tacacacagg gcgtcgctta ttccgccacc gggacattgg
     3721 aaggcccctg gatacaggag aaggagccga tcacaccacc caatttcggg cacgggatgc
     3781 ttttccgcac attgcagggc aaactattga tggcgataca cagccatcag aatatcaacg
     3841 ggcattaccg ccgggtcccg catttgtttg aggtcgatga ctctggtgac aaactgatcg
     3901 ttcgcggacc ctatcatccc tgaagatctt cggtttcccg tatcttttct tcaacgatca
     3961 tcccggaaat tgtaatttcg acctacagcg aatcatcaat tacgcggtaa aattcaaagc
     4021 aatgctgaat accatccttt cgaatttata tgaaaaagac ctacgcgtcc tcatcgaaga
     4081 agtcaaacaa ttcaaggttg aagacaacct ctggcaaact accggctccg tgaaaaactc
     4141 tgccggcaat ctcgcattgc atatcatcgg cggattgaac cattttttcg gagctaccct
     4201 tgcgaatacc gggtatatcc gcacccgcga ccaggaattc agcaaaaagg gcgttaaaag
     4261 agaagacctt ataaccggtc tggaggaagt gatcggtatt gtcacgaata cgctgaacaa
     4321 tctcacacag aaggaactgg aggccgaata ccccctcata ttcgacggtg agaaaaaaac
     4381 caccatgttc atagtcgtgc gcatgtacgc gcatttgagc taccacctcg gacagataaa
     4441 ttatctccgc agaattctgg aatagatatc atgaaaaaga tctttaccgc catttgtctt
     4501 tggtcctgcc ataccctttt ggcccaatcc gaaaaaggtg ccatcgatct gagccagctt
     4561 cagacgcccc agccccacta tcttccggaa tataccttcg atagctctac cgatcctgcc
     4621 cgctggcagg ccgaaaagca tggcctccgc gtatcctttg cttcgaccga catccattat
     4681 ttccgaacgg aagtccccga catagaacaa acaaccgagt ttcgggccgc tggctggaag
     4741 gccgatcgcg tgaatgcgct catcctcgtc tggtcaccgg atacactcga gcaggtacgc
     4801 ttcgtcgtcc atgatctcgt caatacccgg ggccagcgcc tggataaaaa gaatatccat
     4861 ctgaatatgg tccggtatgt ggtctcgaat tatccttata atgcaaagga tgccacctgc
     4921 gacgtttctc catataaaaa ggcattcctt atgcccgatc gtttcgagac atttgagcaa
     4981 ttcgatgtac ccggtcgaac ggtccgcccg gtatggctgt cgctcgacat ccccgccggc
     5041 agcttgcccg gtatttacag cacgacgata gaggccaaat gtaaagggta tagcgtaccg
     5101 ctaaagatca gcataaaggt gcagaatgag cagctgcctc ccccgcacga gtggaaatac
     5161 cggctcgatc tttggcaaaa cccatgggtg gtggcctggt acaatcatgt gaaaccctgg
     5221 tcccccgagc acaagatgct gttgaaaaaa catctcgcct tatacgcatc ggccggcggg
     5281 aaatatatca ctacctatgc cgtgcattcg ccctggtccg ataattctta tatgatcgaa
     5341 gagaccatga tcgaatggat caaacgcaaa tccggttcat ggcaattcga ttatcatatt
     5401 ttcgatgaat acgttcaact ggccatggag gcaggcatcg ataaggccat caccatttat
     5461 acgccggtgc catgggggga taggttccga tacatggacg aaaggacagg tgactacgtg
     5521 tacgagctat ggactcccgg ttccgatgct tttaagagtc attggaatgc atttctcaac
     5581 gatctaaaga aacatctgca acaaaaaggc tggctgaata aaacctatct tgggatcaac
     5641 gaaacaactc tcgacgagac catggcagcc atcagggcca tcagggcgaa ctcacccgat
     5701 tggaagatca cctatgccgg caactggcat aaagaactgg atggcttgct taacgactat
     5761 tgttgtctct atggcaacga accgggtgtc gatgacgtga aagcacgcgc agcaaaaggt
     5821 ttcacatcca cctactacat ctgctgcaat cccccgaaac caaacgattt tatcttttcg
     5881 ccccctgtag aaggccgatg gctcggatgg tatgccgcgg ctcacggata tgacggattc
     5941 ctgcgctggg cctatgacgc ctggcctgca gatccggccc gcgatgcaag acatgccata
     6001 tggcctgcgg gagactgttt cctcgtttat ccgggtggta acagttgtat ccgttttgaa
     6061 cgtctgcgcg aaggcatcgt cgactacgaa aagatcgaca tcttaagaaa aaaggcccgg
     6121 gcatccaccg acccaaagat caaagatctc ttgaaacagc tggatgacca tctgcaaacc
     6181 cttcttgcag agcacgaatt caggacggaa aacgtcattt ccgaggtaga gaaagggaag
     6241 aagatcatag aggaactgag cgatctggaa gggggtaggt catgagaacg ggatccatca
     6301 tcctcgcgat catcattaca ctcaccgcaa ggaccttaaa ggccgcagat agtctatcct
     6361 gtacagaata tgacgattat atcctgaccc atcaattgac accgtttggc ccggtcccta
     6421 ccgccctcga tccgaacggt gtttaccctt atgtcagcta ttgcgaaact tcgaacaggc
     6481 cggcattgaa aaagtatcat tttatcgttt tgcaaaatga cgatataaag atcacagtct
     6541 gccctgatct cggaggaaag atattttcaa tgatccataa accttccggc aaagaagtcc
     6601 tgtatgttcc cgggatcatc cggcaaacca ggatcctgcc aagattttat ttcgtagccg
     6661 gtggcatcga ggtcagtttc cctatttcgc acaccccggt gcagaacgaa aaacttctgt
     6721 accggatcga caggacaccc gatcgtatct atgtcacctg cggagaaaga gaactgagat
     6781 tcggcatgca atggtcggtc gaatattctc tgggtatgaa cgatcccttt ctgaccgaac
     6841 gcgtggtctt tcgtaatccc ggcagtttag cctatccctg gatgtcctgg tcgaatgctg
     6901 cgatacccgc cgctcccgat acccgtttcg attttccaaa aggaagggtt ctgtcacacg
     6961 cctcaaccgt agataccatc gactgggaaa aacagggtcc gcggacccaa tccgatataa
     7021 aggaaatgac gggttatttc tgggagtcca aagacgtcaa tgcattcgga gcattcacaa
     7081 agacacttgg catcggctta tatcatatcg ccgatgcgca tattaccggc gggataaaat
     7141 tgtggagcta tggggtagga gaggacagcg catggtctgt cctgagtacg gcaagtcacc
     7201 aaacctatct cgaattacag ggcgggccct tgagcgacca gtcggtcaag gcggaacttc
     7261 agccaaaaga aacaaaatgg catttagaat actggatccc ttccgaccat cccctggaca
     7321 tctactcgct aaagctaccc aacacgcgat tgagacccgt tacgtccatc ccgctttttg
     7381 aatgggcgag acccaaagaa gtaaaaatat ggaaggacct ggcgggcgca tataacaaaa
     7441 gatcaattcc gcccacccct cccgccatag acgagtattg ctgggcaccc tccgggatgg
     7501 aagaccttga cggagctttt cagtgggcca tccgcaacgg caaaaaggaa caacaacagc
     7561 tttggtggtt ttattatggg acctggctcg caggtcgcgg gatgacggat agcgccatac
     7621 aggctctttc caccagcgat atgggtattg caaaagcact gctggccagg ttataccatc
     7681 tcaaaggcaa taacaatgcg gcggcggacg ctatccgatc catacaggaa ccgtggcttc
     7741 agcgacatcc gcaaatagta gtggaaagag ataaatatct tcgcgcactc ggcccgcaaa
     7801 ccttgcatga acgcgaccaa tcgcttgcaa ccgtagacgc cctcaaggac gaatgggtca
     7861 ttgaaagaaa ggtccagctc ctgatcgata agggtgaatt tcaaaaagcg aaagatattc
     7921 tcctatcgac tccctttcaa aaagtacatc agacctatac ccggactggt ctttggaagc
     7981 agataaccga caaactgaat attccctttc ttcctatccc tgtgtctctc ggggaagaca
     8041 ggctggctca tttcggggca tatcgggaat atgaatagag ggctgggctt gctgcaggaa
     8101 ctccttcctt accaccttcg ccgttttgtg atctcccgta tggctccatc cggggggcat
     8161 cacaatgtat aaaaatttat tcttcaaccc cggcgccgcg tacacatctt tcgacaacgc
     8221 gacgatctct ccgaaataaa catcccaaaa attattcgga ttcattttcc gggtaatccc
     8281 atattcgatc ggtatgtccc ttcgctcgtc ggccagcgtg ccgaataccc ggtcccaaat
     8341 attcaacagg ttacaaaaat tcgtatccat atacaccgga ttacgggcat ggtgtacccg
     8401 atggcggctg ggtgtaagga tgatcttatt caggaacccc aatttaccat ctttcagcac
     8461 attctcacct gcatggataa acgcgcccca ggtgccgtct ataaacataa taaagaacag
     8521 cagtggtgga ttgacgccga gcaaaatgca gatactggtc cggatgacgt cggcataagg
     8581 cgcttcgagg ataaaatgcg cataggtgac cgaaaggttc atcgtctggg gagcatgatg
     8641 ggtactgtgg aggcaccaca acagtcttac cttatgtgcc aggtagtggt agacgaaatg
     8701 cgacaattcc caggcaatat acccgtatat cagccaatac caggtaaagg tggtccggag
     8761 cagaccgtgc ttttggaaca gtgcgataca atatgcagtg gccgcaatgg cgataaacct
     8821 gcccgcaaac tgattaaaca catacgtgaa aaactgcacc ttgtaatcga tgaccttgaa
     8881 tctcctgaag aaggccgcgc gaacgatctc gagcagcagc aaaaaaggga ttacggggcc
     8941 gatcgcattt tcgatgccct gcaaggtatg gagcgagcta tagtcgcctt tgtggtacat
     9001 gtccagcaat ggccctattc ccaaaaaccc gatcagttcc tggtaaagtg ttgcaagaat
     9061 ttttgacatc attgcgaaaa tacggaagaa aatggcgtgt tccgtcgccc aaccgtgtca
     9121 tagttgtata aatacatgca catgacccgg gcttcacaaa gacaggtatg gcttttgggc
     9181 acactcgcaa tatctgcatt tttagtagcg actatcggtt gtctcgatgc cggtcctgac
     9241 ggccccaccc aaaaacatgt tcagtattat gccatgctgg ggacccgggg tatctggcag
     9301 gatggctgga aagcttcggc ggtacatgcc gcgatcaccg gcaaagggaa tttcgataag
     9361 gaccaatggc agctgtatca tgtcgatgcc gaccgctcgg aatcgcagga ctcccctcaa
     9421 caatacccgg acaagctgaa agcccttgtc gatctctggt tcgaggaggc caaaaagaac
     9481 aacgtgcttc cgctcgacga tcggaccgcg gccgaacagc tgggtgtcga tcgaccctca
     9541 caggggcctc cgagagatag atacatttat tatccgggga ccgaagccgt tcccgagggc
     9601 gtcgccgtca atatccggaa ccgatcgtac aagatactgg ccaatgtgga gctgggaccg
     9661 gatgcatcgg gcgtcatttt tgcacatggc tcccgtttcg gcggacattc cctattcata
     9721 aaagaccata aggttttcta cgtttataat ttccttggta tcaggcccga acaggtcttc
     9781 tcgtctccgc cgctgccgcc gggcagatac acgctgggcg tcggattcac cagggactcc
     9841 acaggtaaaa atggagagtc ggtaggtcat gtcaagttat atgtgaatga taaaatagtc
     9901 gcgggaggcc tcatgcggac ccagctgggg aagttcacgc tttccggaga cggtctatgc
     9961 gtcgggttcg atagcggcga cgcggtcagc gatcaataca agacgcctgg aacattccaa
    10021 ggtggggtca tcaatttgtc ggcgtaacgg tcgaaaaaac acaatacatg aacctggaga
    10081 gcgaggcgaa aagggccatg acggccgatt agataaaaag gcgatgcgga cgaacaaaaa
    10141 gatactggcg gctttggcga tactgttcgc actgggttgc ccggcgatgg cccaggacag
    10201 cacagccacc caccagagga cctataagaa cgtgatccgg tatgatctgt cgggaggtct
    10261 tcttttcggt ttcggtaaat acgtcgtgtt cggatatgag cgagtgcttg gcccgcatca
    10321 aagcatgtct gtcaacatcg gacccgccag ccttcccaga cttctctcca tcgagaccga
    10381 cagcttcagc gtgtcgaaag acactaagcg taaggggttc aatgtttcgg tagattaccg
    10441 gttttatctt gctaaggaga acaaatacgg ccctccgcgt gggctttata tcggcccgta
    10501 catctcctac aaccagttcg agcgcaacaa cgagtggaac tacaatagcg caacttcgcc
    10561 acagcaggtc gtgcatacga ccactgagtt caagatattg acgctggggg gtgagattgg
    10621 ttaccaattc gtcatctgga aaagactggc gatcgacatg gtgatgatcg gtcccggttt
    10681 ctcccattat gacctgcatt ccgtgatcca caatacctta agcgagaggg caaaagaaca
    10741 gatccagtcg gctatcaaac aactcatcac ccaaagattc cccgggatga actatgtctt
    10801 ttcggacaaa caattcgatg ctaatggcag gatccgcacc tgggacgtcg ggtaccgcta
    10861 tat
//
LOCUS       DASYSV010000186         7479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_35417, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000186 DASYSV010000000
VERSION     DASYSV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35417"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VGQ51_05645"
     CDS             complement(<1..976)
                     /locus_tag="VGQ51_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036079.1"
                     /translation="MLKNYLLLAFKNFRKHRLFSAVNVLGLTIGITCCLMIFLYVIHE
                     FSYDRWQANGDRIYRAVRVNDVEANNEKKDIAWLSPPYAKALLNDYPDAVQKAVRVRP
                     DNDLITYNSVGFNEKKIYLVDSNFFSFFSFRLLKGDPATVLNDPLSIVMTASAAKKYF
                     GDQDPIGKVVEMNKKLQLKVTGIAADVPGNSTLDFDMVVPLANWRTEYWMNQWPGNSL
                     YTYVQLAPGVDAGRLERQFPTFMDKYLGKYYRENGFKMSLALRPLKDIYFASDYGDNV
                     KHGSRKMVYVFMSIAVLILTIACINFMNLATARANDRSKEVGLRKVMGA"
     gene            1185..1829
                     /locus_tag="VGQ51_05650"
     CDS             1185..1829
                     /locus_tag="VGQ51_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV9036080.1"
                     /translation="MRKLILKMSMSLDAFVAGANGELDWIFKTTDAKSTAWTVDHISN
                     AGLHIMGSKTFHDMAAFWPTSTEVFAAPMNEVPKGVFTKKGFSGADPTRVTRAVADAL
                     KNRAAKGEIASSAPAGADSWAHPRVFDGDLATGIRQLKKEDGAEIIAHGGAGFAQSLV
                     ATGLIDEFRLLVHPVAIGKGLSLFGQLTQRLNLQLAEITPFPAGTVAHIYRPAP"
     gene            complement(1897..3087)
                     /locus_tag="VGQ51_05655"
     CDS             complement(1897..3087)
                     /locus_tag="VGQ51_05655"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEV9036081.1"
                     /translation="MRQLCIFRAMLFCIAFFTALVVRAQKFEKLAATPPMGWNSWNKF
                     ACDGINETVVRETADAMVSSGMAAAGYQYIVIDDCWQVGRDSAGNIVADPVKFAHGIK
                     ALVDYVHSKGLKFGIYTCAGSKTCAGRPASRGYEFQDARQYAAWGVDYLKEDWCNTTT
                     QDAKESYTLMRDALYKARRPIVFSLCEWGSHQPWHWASDVGHLWRTCGDIGNTWNFME
                     AGDGKAWGGSVIGNLDLQSGLEKYAGPGHWNDPDMLEVGNGGLTPEEERSHFTLWCML
                     AAPLMSGNDLRSMSEFTKSILTNRDVIAIDQDALGRQGYRESIKDDLEVFVKPLSGGD
                     TAVCLFNRGEAVRKLDFAWSDYKIGAGHSLRNVWKAAAEGSTSARYKGTIGRHEVILL
                     RLGH"
     gene            complement(3101..3481)
                     /locus_tag="VGQ51_05660"
     CDS             complement(3101..3481)
                     /locus_tag="VGQ51_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV9036082.1"
                     /translation="MTTTAMTMTTAEIANRLVEFCKKGDFEGAQKELFADDAVSIEPH
                     ATADFAQETKGLDAILEKGKKWGEMVEEQHGGSVSDPLVAGDSFAVTISMDITMKGGN
                     RMNMTELCIYHVKDGKIVSEQFFM"
     gene            3689..6100
                     /locus_tag="VGQ51_05665"
     CDS             3689..6100
                     /locus_tag="VGQ51_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036083.1"
                     /translation="MLNNYYKVALRNLRRKRVFSLINILGLSIGLAACLLIAANVYDE
                     THYDRYAARAKDIYRVDLGLTGNTTSGYPMVDVAVGPGMTAVYPDIEAYTRMGHVGDQ
                     FVSYDDRMFKEQKMVFADSNFFRIFTVPFLEGDPRTSLLPPNSLVVTRDFADKYFGTG
                     PALGKTLQVQNYGACKITGVIDRIPDESHFHFDAFLSESSQHFRQQAWTNVGLYTYLL
                     LRPGADPKKLEARFPDLVAKYCVPEIAHDMGVPLSAAQSAVKTFVFSLTPLTDIHLHS
                     DTKYELEANGSSRYVLIFAALAIFILLLACANFTNLSTAAAAGRGKEVGIRKVMGSLK
                     IQLITQFIVESVLLTALSMVLAFFLVSLLLPWFNQVAGKHIPFSFFLGCPALIAAAAL
                     TIIVGVAAGVYPAFFLSSFNTIKVLKSGSAMTGSRRSLLRSSLVVFQFVVSIGLIVAT
                     LIVYRQLRYMQDKRLGFDQNQVIAIEDANLLGKNQDAFKQQLLQDRRVVNASISWSVP
                     GSGAMNGTEIYPKQDGPKNGREIHTNIYDIDYDYVPTLGLQIVRGRNFSRDFPTDSTG
                     VLINETEARDLGWGNTDPVGKTIIRSGQLEYKVIGVVRDFHYMSVKMKIAPLMMLLGN
                     NYGGILVKANTADIGSFLADARQHWKTYNPGAPFSYYFLDERFATMYVAEQRTGRLFT
                     AFTIIAILIAGLGLFGLAAYMVEQRTREIGIRKVLGASVSSVLLLVSKEFLLLVGVAF
                     IIAVPVTWWAMSEWLHEFAYRTPVSWWIFPLAGAAALLIALFTISFQTTRAATSNPVD
                     SLRAE"
     gene            6163..>7479
                     /locus_tag="VGQ51_05670"
     CDS             6163..>7479
                     /locus_tag="VGQ51_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036084.1"
                     /translation="MFKNYLRIALRNLTRNRFSSIINIGGLAIGMAVSILIGLWVYMH
                     FSYNRSIPNHKRIAAVLQNQLLSGNTVTWRGQARQLAPALQKDYPRLFKHVAVIAGPT
                     SRPVAYGDTRITQKGVYTDPGIIDMMSLNMIKGDTGSLSDQSSIILAASTAKSLFADA
                     DPIGKIVKVNNDQLLKVTGIYADPPENSDLADFNFIIPFEYLAHTDSTVAHLNWGNSW
                     FGTYVQLNDNVDMAAASRAIKDVKYRYAEGDRRFKPELFLFPMDDWHLRSEFKQGVAA
                     GGFIQYVRLFSTIGIFVLLLACINFMNLSTARSEKRAKEVGIRKAIGSMRGQLIRQFF
                     TESVLTALVAWFAALVIAQLLLPFFNELANKKMIIPYRQPMFWAAGFIFVFITGLLAG
                     SYPAIYLSSFRPVKVLKGDLTRSRTRPSRWLGRSFIKAGRLAALPRQ"
BASE COUNT         1683 a         2349 c         1721 g         1726 t
ORIGIN      
        1 acgcacccat caccttccgg agacctactt ctttgctccg gtcgttggcc cgcgcagtgg
       61 caagattcat gaaattgata caggcaatgg tgaggatcag taccgcgata ctcataaaga
      121 catagaccat tttcctgctg ccgtgtttta cattgtcgcc atagtcgctc gcgaaataaa
      181 tatccttcaa cggccgtagc gcaagactca tcttaaaccc gttttcccgg taatacttgc
      241 cgagatactt gtccatgaac gtcgggaact gccgctccag ccgtccggca tcgacacccg
      301 gggccagctg cacataggta taaagactat tgcccggcca ctgattcatc cagtattcag
      361 tccgccaatt agccagaggt acgaccatgt cgaaatctaa agtggaattg ccgggaacat
      421 ccgccgcaat tccggtcacc ttcagctgca attttttatt catttcgacg actttgccga
      481 tcgggtcctg gtcgccaaaa tatttcttcg cggcggatgc cgtcatgacg atgctcagcg
      541 ggtcattcaa caccgttgcc ggatcgcctt tcaaaagacg aaaactaaaa aagctgaaga
      601 agttgctgtc caccaggtag atcttctttt cattgaagcc aacgctattg tacgttatca
      661 gatcgttgtc cggccggacc cgcaccgctt tctgaacggc atccgggtaa tcgttcagca
      721 aggccttcgc ataaggaggc gacaaccacg cgatatcctt tttttcattg ttggcttcca
      781 catcgttgac ccgcaccgca cgatagatcc tgtccccatt cgcctgccac cggtcataac
      841 tgaattcatg gatgacatac aggaagatca tcaggcaaca cgtaatgcca atggtcagtc
      901 caaggacgtt gaccgcggag aacaaccgat gtttacggaa gttcttaaag gcgagcagca
      961 gataattttt gagcatactt ctgtcctgtc caaagctgat gccgttttcc caatcatttg
     1021 cccgacaatc cattatgacc cgctccaacc ccttttctgt tcgctttcga tacaggcgat
     1081 gtccggccct gtcacacatg cgttttaacc catcggcttt gtcgcataac ccccgcaccc
     1141 gcttcccgca ccatcactaa atttggatca ttaaattttc aaccatgcgt aaactcatcc
     1201 tcaaaatgtc catgtccctc gacgcattcg tcgccggagc caatggtgaa ctcgattgga
     1261 tcttcaaaac caccgacgcg aagtccaccg cctggaccgt cgaccacata tcgaatgcag
     1321 gcctgcacat catgggcagc aagacctttc atgatatggc ggcattctgg ccgacctcga
     1381 ccgaggtctt cgccgcgccg atgaacgagg tacccaaagg agtattcacc aaaaaaggat
     1441 tttccggcgc cgatcccacc cgcgtgacgc gagcagtcgc cgatgcactt aaaaatcgcg
     1501 cggcaaaagg agagatcgct tcgtctgctc ccgcaggagc cgacagctgg gcacatcctc
     1561 gcgtgttcga tggtgatctg gcgaccggga tccgccagct gaaaaaagag gatggcgcgg
     1621 aaatcatcgc acacggaggc gccggtttcg cccaaagtct cgtcgccaca gggctcatcg
     1681 acgaatttcg gttgctggtc catccggtag ccatcggcaa aggtctgtcc ctcttcggcc
     1741 agctgactca acgactcaat cttcagctgg cggagatcac accctttccg gccggcaccg
     1801 tcgcgcatat ctatcgtccg gctccataac acaccccggc accgtcgcgc acatctaccg
     1861 tggcgcttcc cgtctcgcat accatccggc gaccctctag tgacccagcc gcaacaggat
     1921 cacctcatgc cgccctatcg tccccttata ccgcgcggac gtcgaaccct cggcggccgc
     1981 cttccataca tttcgcaacg aatgccccgc gccgatctta tagtcggacc acgcgaaatc
     2041 caatttccgg accgcttccc cgcggttgaa caaacaaacc gccgtatccc cacccgacag
     2101 tggcttcacg aatacttcca gatcgtcttt gatcgactct ctgtaaccct gacgacccag
     2161 cgcatcctgg tcgatggcaa tgacatcccg gttggtcagt atactcttcg taaactccga
     2221 catgctcctc agatcattcc ctgacatcaa cggcgcggct agcatacacc acagagtaaa
     2281 atgcgaacgt tcttcttcgg gtgtcagacc tccgttgcct acctcgagca tgtccggatc
     2341 attccaatgt cccggaccgg catatttctc gagtccgctt tgaagatcga gattaccgat
     2401 gacactgcca ccccaggcct tgccatcacc cgcctccatg aaattccagg tattgccgat
     2461 gtcgccgcag gtacgccata gatgaccgac atcggacgcc caatgccagg gctgatgaga
     2521 gccccattcg caaagactga atacgatggg ccggcgtgct ttgtacaggg cgtcgcgcat
     2581 cagcgtataa gattccttcg catcctgcgt agtagtgttg caccagtctt ctttaagata
     2641 atctacaccc catgccgcat attgacgggc atcctggaat tcataacccc tgctcgcggg
     2701 acgacccgca caggtcttac tccccgcaca ggtatagatc ccgaatttta gccctttcga
     2761 atgaacatag tcgaccaaag ccttgatgcc atgagcgaat ttgaccggat cggctacgat
     2821 attacccgca ctgtccctgc ccacctgcca gcaatcgtcg atgacgatgt attgatatcc
     2881 ggccgctgcc atgccgctgc ttaccatcgc gtcggccgtt tcgcggacca ccgtttcatt
     2941 gatgccgtca caggcgaatt tattccagct gttccatccc atcggcggtg tcgccgccag
     3001 cttctcgaat ttctgagcac gaaccactag ggcagtaaaa aaggcgatgc aaaaaagcat
     3061 cgccctgaaa atgcagagtt gacgcataat gagtgtgtgt ttacataaag aactgttccg
     3121 aaacgatctt accatccttt acgtgataga tgcacagttc cgtcatgttc atccggttac
     3181 cgcctttcat cgtaatgtcc atgctgatcg ttacggcaaa agaatcaccg gccaccagcg
     3241 gatccgatac tgaaccgcca tgctgctcct caaccatttc cccccatttc ttacccttct
     3301 cgaggatagc atccagtccc ttcgtctcct gcgcaaagtc agcagttgca tgcggctcga
     3361 tactcacggc atcatcggca aacaactctt tctgcgctcc ttcgaaatct cctttcttgc
     3421 aaaactctac cagacgattg gctatctcag ccgtcgtcat tgtcatggct gtcgttgtca
     3481 taaaaataaa ggttttaagg aaagttagtg caattccggc acaagaccgc ctccctccgt
     3541 acacccggcg tatcgaaacc ggacaactca ccctcaccgg cgcaacaagt ctatctgatt
     3601 ttcaataatt taccgatctg gcattttttt agccctccgc ctctccgggc gtaataattc
     3661 ccgcctccta tcctcaataa ttcctcacat gctcaacaat tattataagg tcgccttgcg
     3721 gaacctccgg cgcaaacgcg tcttctctct cataaacata ctcggcctct ccatcggtct
     3781 ggccgcctgt ctgctcatcg ccgccaatgt ctacgatgag acgcactatg accgctatgc
     3841 cgcccgcgca aaagatatct atagagtgga ccttggcctc acgggcaata ccacctccgg
     3901 ctacccgatg gtcgatgtcg cagtcggacc cggaatgacc gccgtctacc cggatatcga
     3961 ggcgtatacc cgtatgggac atgtcggtga ccaattcgtt agctacgacg accggatgtt
     4021 caaagaacaa aagatggtct tcgccgactc caacttcttc cgcatcttta ccgttccctt
     4081 cctggaaggc gacccgcgca cctcgctgct cccgcccaat agtctcgtcg tcacccggga
     4141 tttcgcagac aaatatttcg ggacaggacc cgcgctcggc aagacccttc aggtacagaa
     4201 ctatggcgca tgtaagatca ccggcgtcat cgaccgcatc cccgacgagt cgcatttcca
     4261 tttcgatgcc tttctcagcg agtcctcgca acatttcaga caacaagcct ggaccaacgt
     4321 cggattatat acctacctgc ttctgcgccc cggcgccgat ccaaaaaaac tcgaggcccg
     4381 tttccccgat ctcgtcgcca aatactgcgt tcccgagatc gctcacgata tgggcgttcc
     4441 cctgtccgcc gcgcaatctg cggtcaaaac cttcgtcttt tccctgacgc cactgacgga
     4501 tatccatctc cattccgata ccaaatacga actggaggcc aacggcagca gccgttatgt
     4561 cctcatcttc gccgccctcg ccatctttat cctgttgctg gcctgtgcga atttcaccaa
     4621 tctttccacc gccgccgccg ctggccgcgg aaaagaagtc ggtatccgaa aggtcatggg
     4681 ctcgctcaaa atacagctca tcacccaatt catcgtggaa tccgtcctgc tgactgccct
     4741 ctcgatggtg ctggcctttt tcctggtgag tctgctcctg ccctggttca accaggtcgc
     4801 gggtaaacat attcccttct ccttcttcct cggttgcccg gcactgatag ccgcggcggc
     4861 attgacgata atagtgggcg tggcggcagg cgtatatccc gcctttttcc tttcttcatt
     4921 caataccatc aaagtactca aatccggctc tgccatgaca ggttcgcgcc gaagcctcct
     4981 gcgcagcagc ctcgtcgtct tccagttcgt agtctcgatc gggctgatcg tagcgacgct
     5041 catcgtctat cgccagctgc gttatatgca ggacaaacgt ctcggtttcg atcagaacca
     5101 ggtcatcgcc atagaagacg ccaatttact cggaaagaac caggacgcgt tcaaacaaca
     5161 gctgttgcag gaccggcgcg tcgtgaatgc cagtatcagc tggtccgtgc cgggcagcgg
     5221 cgcaatgaat ggcacagaga tctaccccaa acaggacgga ccgaaaaatg gccgtgagat
     5281 ccacaccaac atttatgaca tcgactacga ctatgttccg accctcggac tgcaaatagt
     5341 acgaggaaga aatttctccc gcgacttccc taccgactcc accggcgtcc tgatcaacga
     5401 gaccgaagcc agggacctcg gctggggaaa cactgacccc gtcggaaaga ccatcatcag
     5461 gtcgggccag ctggaataca aggtcatagg cgtggtccgc gatttccatt atatgtcggt
     5521 caaaatgaag atcgccccgc tgatgatgtt gctcggcaat aactacggtg gcattctcgt
     5581 aaaggcgaac acggcggata tcggcagctt cctcgccgat gccagacaac attggaagac
     5641 ctacaacccc ggcgctccct ttagttatta tttcctcgac gaacggttcg ccacgatgta
     5701 tgtggccgag caacggaccg gcagactatt caccgccttt actataatcg ccattctcat
     5761 tgcgggtctg ggcctgttcg gactcgccgc ctatatggtg gaacaacgga cacgggagat
     5821 cggcatccga aaagtgcttg gcgcctccgt atcttcggtc ctgctcctcg tctcgaaaga
     5881 attcctgtta ttggtaggcg tggccttcat tatcgcagta ccggtcacat ggtgggcaat
     5941 gagcgaatgg ttgcatgaat tcgcctaccg gacacccgtt agctggtgga tcttcccgct
     6001 ggccggcgca gctgccctgc tcatcgcctt attcacgatc agttttcaaa cgacccgcgc
     6061 ggctacctcg aaccccgtcg atagcctccg cgcggaataa cagcgcggcc gaaaccttca
     6121 gtcggatcct ccggctctgg cggccgcccc aaacaacttc ttatgtttaa aaattatctg
     6181 cgcatcgcgc tccgcaacct gacccgcaac cgattctcct cgatcatcaa tatcggcggt
     6241 ctcgccatcg gcatggcggt atccatcctc atcggcctgt gggtatacat gcacttctct
     6301 tacaacagat ccattcccaa tcataaacga atcgccgccg tcctccagaa ccaattgctc
     6361 agcggcaaca cggtcacctg gcggggccag gccagacaac tggcgcccgc tctgcaaaaa
     6421 gactatccgc gattgttcaa acatgtggct gttatcgcgg gtcccaccag tcgccccgtt
     6481 gcctatggcg atacccgcat tacccaaaaa ggtgtttaca ccgaccccgg catcattgat
     6541 atgatgagcc tgaatatgat aaagggtgat accggttcac tgtccgacca gtcttcaatc
     6601 atactcgccg catcgacggc caaaagcctt ttcgcggatg ccgaccccat cggcaagatc
     6661 gtaaaggtga ataatgacca gctgctaaag gtgacgggca tctatgccga tcctccggaa
     6721 aactccgatc tggccgactt taattttatc atcccctttg aatacctcgc ccacacggat
     6781 tccaccgtcg ctcatctcaa ctggggtaac agctggttcg gtacctatgt tcagctgaac
     6841 gacaatgtcg atatggccgc cgcctcccgc gcgataaagg atgtcaaata ccgttatgcc
     6901 gaaggagatc gcaggttcaa acccgagctc ttccttttcc ccatggacga ttggcatctc
     6961 cgttccgaat tcaaacaagg cgtcgccgcc ggcggcttta ttcaatatgt ccggctattc
     7021 tcaaccatcg gtatcttcgt gctccttctc gcctgtatca attttatgaa cctcagtacg
     7081 gcccgctcgg agaaaagggc caaagaggtc ggcatccgga aggccatcgg ctcgatgcgc
     7141 gggcagctga tccggcagtt ctttaccgag tcggtgctca cagccctcgt cgcatggttt
     7201 gcagcgctgg tcattgctca attgctgctt cccttcttca acgagctggc caataaaaag
     7261 atgatcatcc cctatcgtca gcccatgttc tgggcggcgg ggttcatctt tgtattcatc
     7321 accggtctgc tcgccggcag ctatcctgcc atctatctct cttctttccg ccccgtcaaa
     7381 gtgctaaaag gggacctgac aaggtcccga acgagaccga gccggtggct cggccgaagc
     7441 ttcatcaaag ccggcagact ggcggctctt ccacgtcag
//
LOCUS       DASYSV010000187         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_672768, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000187 DASYSV010000000
VERSION     DASYSV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672768"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VGQ51_05675"
     CDS             <1..924
                     /locus_tag="VGQ51_05675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV9036085.1"
                     /translation="SEAHRKDCYMPVLHYSNCPSCRCENLKPALNAIDHTVSHESFAI
                     WECDRCSLRFTQDVPDEGSIGPYYKSDSYISHSNTSKGFVNSLYHMVRRETLAEKYRL
                     VASATRLKQGKLLDIGAGTGAFVGYMQGCGWEVTGLEPDASARSVARTDNEVQLLDIG
                     QLFSLTPDGFDAITLWHVLEHVHDLHGYMEQLKKLIRRSGRIFIAVPNYTCYDASVYR
                     ENWAAYDVPRHLYHFSPMAMQTLVVGHELQLLGSQPMWYDSFYISLLSEKYRGNGRGN
                     MLRAGVNGLVSNAKAFVNKSRCSSLIYVISK"
     gene            complement(916..1800)
                     /locus_tag="VGQ51_05680"
     CDS             complement(916..1800)
                     /locus_tag="VGQ51_05680"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEV9036086.1"
                     /translation="MYKLSLFLLTTGMFVACNSKPDTQAPENSDNTPPPVSYSVVKAY
                     PHDTSAYTEGLLFHDGQLYESTGTEAVDMPDSRRSLFGTVDLNTGRITPKAELDRQKY
                     FGEGIVFLHDKIYQLTYTTKIGFIYDAKTFKKIGEFTFPNKEGWGMTTDGKYILMSDG
                     SSDISYYNPDSTKPVSVMIHYDNGDSTQKTLNSLSLVKTLNVTDNNGPASNINELELI
                     HGYLYANQWQTNYILKIDTATGKIVGRLNLDSIANDAKSKYPGSEWMNGIAYDPATNK
                     VYITGKLWPNIYEIQFSF"
     gene            2094..3365
                     /locus_tag="VGQ51_05685"
     CDS             2094..3365
                     /locus_tag="VGQ51_05685"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9036087.1"
                     /translation="MKYLLSLVGYLLIALSGWTQPVCRSFDYRQRQLSAHPGLASVVN
                     GIEQFTREKLQERTVGVTGQEAPLSTLPVITIPVIVHVLYNNSAENISDAQIQSQIDE
                     LNRDYQKLNPDTSRIPSYFSSVSTDCGFRFILAGVDTNGRIITGVIRKHTNITAFSIE
                     DDMKFSARGGDDAWDRDQYLNIWVCNLANNVLGYSSVIGGAKATDGIVVLYTAFGTTG
                     TAKAPFNLGRTCTHEAGHWLNMIHTWGDDSCGTDFVADTPPQQMATYGDPNGIVLSCN
                     NAPTGNMYMNYMDFTDDMGMHMFTNGQRDRMRTLFAPGGFRYPILSSPAASAAPGEPA
                     DTAYSGIGLSVGSARVYPNPAVSEVTVHLADLSKVGSLLEVYNQEGQRVMGTRVTSQD
                     FNLDVSGLSGGIYFIRLSNGGGQQSLKLVKM"
     gene            complement(3355..>3741)
                     /locus_tag="VGQ51_05690"
     CDS             complement(3355..>3741)
                     /locus_tag="VGQ51_05690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9036088.1"
                     /translation="PIDNLPFKGTFHYDEGFYYFRDLGDRVLLGGARNLAIEEENTDE
                     MILSDKIQTTLERFLREVILPGRSYKIDCRWSGIMGMGAEKMPILKAINDHVFCAVRM
                     SGMGVALAPVIGEKVARLMTGGNPTS"
BASE COUNT          821 a          844 c         1098 g          978 t
ORIGIN      
        1 tctgaggccc accgaaaaga ctgttacatg cctgtattgc attattccaa ttgtccttcc
       61 tgtcgatgtg agaatttaaa gccggcgttg aacgctatcg atcatacggt atcgcacgag
      121 tcgtttgcca tctgggagtg tgaccgctgc agtctgcgat ttacgcagga cgtaccggat
      181 gagggttcca taggacccta ttataaatcg gacagctata tctcgcacag taataccagc
      241 aagggttttg tcaatagcct gtatcatatg gtgaggcggg agacgctggc ggagaaatac
      301 cgtctggttg cctccgctac gcggttgaag cagggcaagc tgctggatat cggggcaggt
      361 acaggtgctt ttgtcgggta tatgcagggc tgtgggtggg aagtgacggg gttggaacct
      421 gatgcgagcg ccaggagcgt ggcccggacg gacaacgagg tacagttgct ggatataggg
      481 caattgtttt cgctgacgcc ggacggattc gacgcgatca cgctttggca tgtgctggag
      541 catgtacatg atctgcatgg atatatggag cagctaaaga agctgatccg tcgaagcgga
      601 aggatcttta tcgccgtgcc gaactatacc tgctatgatg cttcggttta tcgtgaaaac
      661 tgggcggcgt atgacgttcc gcggcacctt tatcattttt caccgatggc aatgcagacg
      721 ttggtcgtcg ggcatgagtt gcagctgctg gggagtcagc cgatgtggta tgatagtttt
      781 tatatttcct tactgagtga gaaatatcgc gggaacgggc gcgggaatat gttgcgcgcg
      841 ggtgtcaatg ggttggtttc gaatgcaaag gcatttgtca ataagtcgcg gtgcagcagc
      901 ctgatctatg tgatatcaaa atgagaattg gatctcgtag atgttcggcc agagtttacc
      961 tgtaatatat actttattcg tagcggggtc gtaggcgatg ccgttcatcc attcggagcc
     1021 ggggtatttg cttttggcgt catttgcaat ggaatccagg ttgagccggc ccacaatctt
     1081 cccggtcgcg gtgtcgatct taaggatata gttggtctgc cattggttgg catagagata
     1141 gccatggatc aattccagct cgttgatatt ggacgcgggg ccgttgttgt cggtgacgtt
     1201 gagcgttttg acgaggctca ggctgttgag ggttttttgc gttgaatcgc cgttgtcgta
     1261 atggatcatg accgacacgg gtttggtgct gtcggggttg taatagctga tatcgctgct
     1321 gccgtcgctc atcaggatgt atttcccgtc ggtggtcatg ccccatcctt ctttgttggg
     1381 aaaagtaaat tcccctattt ttttaaaggt tttggcgtca tatatgaaac caatttttgt
     1441 ggtataggtg agttggtaga tcttgtcgtg gaggaaaaca atcccttctc cgaaatattt
     1501 ctggcgatcg agctcggctt tcggcgttat gcggccggtg ttgaggtcga ccgttccgaa
     1561 cagggaacgg cggctatcgg gcatgtcgac cgcctcggtg cctgtgcttt catagagctg
     1621 accgtcatgg aacaaaagcc cctcggtata ggcgctggta tcgtgcgggt aggctttaac
     1681 aacagaatag ctgaccggtg gtggagtatt atcgctgttt tcgggggcct gggtatcggg
     1741 tttcgagttg caggctacga acatcccggt ggtgaggaga aaaagggaca atttgtacat
     1801 agtaagcaaa aataagggca aaagatgcgg aaaatgcttg caaatgtgtg aaaaagtttg
     1861 ttgttttaca gcttattagc atcggatagc gccactactg caccacacca ccacaattct
     1921 cttctcatta ggtgttatcc ggcatctggt ttgttgacca taatgtatcc ttcctgaaga
     1981 ccaatccaat ttctgccgag gctctcatac ggcacgtggc ttccttttac cgactttcag
     2041 atcaaagttt atttccattc ctatgatggg ccatctctga aaaagaaggc aacatgaagt
     2101 atttactatc ccttgtgggg tatttgctga tcgcattgtc cggctggacc cagcctgtct
     2161 gccgatcatt tgactaccgt cagcggcagc tgagcgcaca tcccggcttg gcgtcggtgg
     2221 tcaatggcat cgagcagttt acgcgggaga agctgcagga gcgcaccgtg ggggtgaccg
     2281 gtcaggaagc cccattatcc actctccccg tcattaccat tcccgttatc gtccacgtac
     2341 tttacaataa ttcggcagag aacatcagcg atgcgcagat acagtcccag atcgacgagc
     2401 tgaacaggga ctatcagaaa ctgaatccgg ataccagccg tatcccttca tatttcagtt
     2461 ctgtttctac agattgcgga ttccggttca tactcgcggg tgtcgacacc aacggcagga
     2521 tcattaccgg ggtcattcgg aaacacacga atattaccgc cttttccata gaagacgata
     2581 tgaagttctc tgccaggggc ggggatgatg cctgggacag ggaccaatac ctcaatatct
     2641 gggtgtgcaa tcttgcgaac aacgtgttgg ggtattcgag tgtgatcggt ggggcgaagg
     2701 cgacggatgg gatagtggtc ctttatacag cctttgggac gacgggtacg gcgaaagcgc
     2761 ctttcaatct cggccggacg tgcacgcatg aagcggggca ttggctgaat atgatccata
     2821 cctgggggga tgacagctgc ggaactgatt tcgttgccga cacgccgccg cagcagatgg
     2881 ctacttatgg cgatccgaat ggtatcgttc tttcatgcaa taatgcgccg accgggaata
     2941 tgtatatgaa ctatatggat tttaccgatg atatggggat gcatatgttc acgaatggtc
     3001 agcgcgaccg tatgcggacc ttgtttgcgc cggggggatt ccgttatccc atactgtcgt
     3061 cgccggcggc gtcggctgcg ccgggagagc cggcggatac ggcgtacagc ggtatcggct
     3121 tgagtgtagg gagtgcgcgg gtttatccca atccagcggt aagtgaagtg acggtgcacc
     3181 tggcggattt gtccaaggtc ggcagtttgc tggaagtgta taaccaggag gggcagcggg
     3241 tgatgggtac gagggtgact tcgcaggatt tcaatctgga tgtttccggg ttgtcgggcg
     3301 ggatctattt tatccggctg agtaatggtg gcgggcagca gagtttgaaa ttagtcaaga
     3361 tgtagggttg ccgccggtca tgaggcgggc gactttttca ccaataacgg gagcgagggc
     3421 cacgcccatt ccgctcatgc gcacggcgca gaatacgtga tcattaatgg ctttgaggat
     3481 gggcatcttt tctgcgccca tacccatgat gccactccac cgacagtcaa tcttgtaact
     3541 acgacccggc aggatcacct cgcggaggaa gcgctccagc gtcgtctgta tcttgtccga
     3601 aaggatcatt tcatcggtat tttcttcttc tatagccaga ttgcgggctc cgcccagtag
     3661 taccctgtca cccaggtcgc ggaaatagta gaatccttca tcataatgaa atgtgccttt
     3721 gaaaggaagg ttgtcgatcg g
//
LOCUS       DASYSV010000188        19070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_290024, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000188 DASYSV010000000
VERSION     DASYSV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 19070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19070
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290024"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="VGQ51_05695"
     CDS             <1..1855
                     /locus_tag="VGQ51_05695"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEV9036089.1"
                     /translation="AFDTYNDERNAMVFATNAYGVQRDLLSFDDLYYDIDWDGLWNVR
                     TNRTDSGWTAEMAIPWKTLRYPGTKDTTQSWGFNVYRNRRLTNEISAFAPFPRIFSAT
                     HMDYAGRLTNLQPPPPSTNITFDPYVLTQVDHYSNFGNAEPSHASTIKFGGDLKWALT
                     PNSVLDLTAHTDFAQADVEQQVNNTSRFNVFFPEKRSFFLENASLFGVQVNMNVDNSG
                     GSMRYQPFNSRTIGLDSIGNPVPVVGGGRFVHRSATTNYGAIAIRQDGVNGEEPTNFF
                     VGRFSRNFAKQDRIGALVTVKNQSAGTNIESTLDGFFRLGESNSINAIFTQTTTSTTG
                     AKGFGGIVQYFNTNNNHEIWLTESLFTRDFDPQMGFLSRSDVIGTTPGMNWFYRGRLL
                     PFKKVMLSYAPGITPELYFTASTRQFSEADVPIYPIWFNFKDGSFIGYGFSMIWQRLA
                     SAFSPLDITIAPGDYHYTNHQVFIGTNPSRMVSGQFMLTAGSYFNGHLITADYKLAFA
                     PIPNISLQGEFNRNHFDKVGINMATQTVDLYILQCRLALNPRLQFTGIYQKNSINSSD
                     AYNLRLAWEFSPLSYLYLIYNRGINNQLAQSPVKSQTEDHLIFKASYLKQF"
     gene            1972..2862
                     /locus_tag="VGQ51_05700"
     CDS             1972..2862
                     /locus_tag="VGQ51_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV9036090.1"
                     /translation="MPTRRNFIKQASLASAGFAFTKNGLSPNKQLIGLQLYTVRDQIA
                     KDPDGVLGKVAAIGYNSVELMGYGNGKYFGLTPAQLAALLKKHRLVSPSGHYMMMNYF
                     MKNDRNDLKDNIAAAVTLGHKFITIPFLTDNMRTSLDDYKRLAAKFNEAAVEAKKSGL
                     RLAYHNHDFEFKDWGGGKTGFGILLKETDPSLVFFEMDMYWVVRAGHNPIQLIRANPG
                     RFKMWHIKDMSQKEPASYTVEGQQYFAEVGAGIIDFKDIFRHKKESGMEYFFVEQDQT
                     SEPVFDAITKSYRYVKTNLA"
     gene            2912..4591
                     /locus_tag="VGQ51_05705"
     CDS             2912..4591
                     /locus_tag="VGQ51_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV9036091.1"
                     /translation="MADNHFDVIVVGSGISGGWAAKELTEKGLTVLLLERGRNIEHVK
                     DYVNATKAPWDFPHRGSKTQQMIKNYPVLNRDYPLNETNLDFWTNEQESPFTEIKRFD
                     WFRGYHVGGRSLMWGRQSYRWSDLQFEANAREGIGVDWPVRYADIAPWYDHVEKFAGI
                     SGSKEGLPQLPDGRFMPAMDMNIVEKDLAARFNDHYKDHRKMIIGRTANITEPLPGRT
                     NCQYRDKCWLGCPFGAYFSTQSSTLPAAMATGKLTLRPWAIVTRVLYDKDKKRATGVE
                     IMDAETNKTYTYTSKIVFLNASTINTAWLLMNSATDVWPDGLGSSSGQLGHNLMDHHL
                     GVGAFGRVEGYEDKYYYGRRANGIYIPHFRNLFGDKRDYIRGFGYQGGAGRDGWDRSI
                     AELSIGSDLREALSEPGNWTFGIGGFGETLPYHENKVTLDKTRKDKWGLNIPAIDAVL
                     RDNELKMRKDMQGDAVEMLTTVGVKDVQGFEGNAVLGRGIHEMGTCRMGKDPKTSVLN
                     KWNQVWDAPNVYVTDGSFMTSANCCNPSLTYMAFTARAANHAVDELKKMNL"
     gene            4603..5139
                     /locus_tag="VGQ51_05710"
     CDS             4603..5139
                     /locus_tag="VGQ51_05710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEV9036092.1"
                     /translation="MQRREALQRVAVLVGGTVIGAEAFLSGCKTPPRKEGLFYDTDVA
                     LMGEIGETILPAGGGVPGAKEAGIGEFMKVIVTDCYTPGDQDVFVKGLQTLRDKKFMD
                     LSAGQRHDLLVELDKEARDPKYNDKNKHYFKMLKQLTIWGYFSSEAGATKATRYIETP
                     GHYDGNLPYKKGDKAWAT"
     gene            5224..6102
                     /locus_tag="VGQ51_05715"
     CDS             5224..6102
                     /locus_tag="VGQ51_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV9036093.1"
                     /translation="MMAKKSILSYGLIPLTFLFLGQPANGQSPDSSGSPRRYHVNYVT
                     GSIIIGIGLGTDAFAISRIQDKPNLTAAELQALNPGVLNSFDRWALHQDPSGYKHYSK
                     ISDVIEPPIFILLPALLALDKKIPKKEWMDILFMYIEGHTITFTFYNYSWLGPTFQNR
                     FRPLTYYSQLPLADRMSGGNRNSFYSGHTASVAFTTFFIAKVFCDYHPDLGFGKYLLY
                     TGALIPPVVMGYFRVKALAHFPSDDLVGLTLGAVVGIVLPALHKANYKGVAINLYSNP
                     ESTGLNVCWTMPDHKK"
     gene            6182..9169
                     /locus_tag="VGQ51_05720"
     CDS             6182..9169
                     /locus_tag="VGQ51_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9036094.1"
                     /translation="MRFLLLIILTLGCTAAASPQIPTSFEQPDVLVKAGVKRVKCILK
                     DSRGFLWIGTETGLFRFDGTNLDLMQHEADSALGLPNSRIAGLAEDTSGNIWVGTMHG
                     AARLDPWTLVCTVYTPQQKNIDLDFDVKPMISNDGRVWLGGNDGLCVLEGGRFRTVTH
                     AEANAPPNAWYVNSMTQWKGDTMAVGTFDGLVLYNMRNGGFTRLFGGRHMTVTKLHVD
                     RRSRLWMGTWGGGCFMLDSNGRSYSRLAPDKPVHGEFRNIITGILETDSREAPHFWIS
                     TEAGVYQVEDPAEQQEGKPECRQIADKGAACIAGDNARYIWIGGQTVTRVFAGHSGFG
                     ILPATIKGSVAAIQPVTYDGQPAVAFSTWYVSGGLTVTNPSGTTTYYRRKARGDAENV
                     SGLATDSLGRHWVSTFDGLDILDQRFMPVRGADTLFRARDKLVTKRTYGVLIHRDTAW
                     VAYYRHGLSLYDMHFHLLHSFSKNDGSGLADDLINRMVADKVGRVWLGGGKGLYEYDA
                     AAGRFRLFNLNPDGSPFEVSDIATLPGGDLILSTTSGIFRFSPATGASSKIRSPLFPD
                     NVIETAAVDKGGDIWFMNREHLVYYQVNSGHFTLFGTEDGLDTRQDLSTLRCPDGEHF
                     YLAGDHLIYTFNRNIRNIAAQPVPLYLHSIQVDDSTLPRNAGVPPLHLNYKQDRITIE
                     FGAINLVKPEQNLYAYMLTGVDTRWIYSTRNYASYANLAPGGYTFRLKVENDAGTWSD
                     VLSLPVAIHPPYWATWWFRLLGLTLIGVAVVLSVRYVVQRNLRERILRLQREQAVEKE
                     RNRIARDMHDDLGSGLTKIAIMSEVTKAQLAVPGAAVTNLDVISNASRELVDNLQDIV
                     WVLNPRNDSIGSLLLYLKEYVEDFFEPTGISCTVVANTVAHDIPISEEKRRNIFLTVK
                     EACNNILKYAECKEVGLAVRIDSRRLTIKLEDNGNGFDIREVGPFANGLKNMRNRIEQ
                     IGGRFQMESEKEIGTIVNIEVPV"
     gene            9240..9872
                     /locus_tag="VGQ51_05725"
     CDS             9240..9872
                     /locus_tag="VGQ51_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9036095.1"
                     /translation="MIQVAIVEDIREIREGLQMLINFSDSFNCSQVYASGEDALKGLP
                     TENPDVVLMDINLPGMTGVECVRRVKPLCPATQFIMSTVYEDDENVFESLKASATGYL
                     LKKTVPARILEAIAEVHQGGSPMSTQIARRIIVTFQKKDGIAAADELTSREKEILKSL
                     SEGLRYKEIAARFRISMDTVRSHARNIYEKLQVQSRTEALNKVYGKDRRV"
     gene            9931..11172
                     /locus_tag="VGQ51_05730"
     CDS             9931..11172
                     /locus_tag="VGQ51_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9036096.1"
                     /translation="MKKTLSVLFALLIAVALQAQVENPGTAAKNGATDHANNDINNSI
                     NNGLNKTEGAIKGLFKKKKKDAAATQPPASTQSAIPTQPAIAPGTASPTDSTQPGQGL
                     AVYKNYDFVPGDKIIFQSALSDESVGEIPSQLTLNKGQMDIQQEDGENVIHIPTGPGA
                     NFSPRMKNMQYMPDQFTIEFDVKNERFGLDHFSIYFGDYRHDDGSLQILRITDASMTW
                     TAGNVEAPRGLDLKKPMTWHHIAIAINKNVGKVYVDQFRFISINNLEGKANQITFDVN
                     GYENSFIKNVRIAAGGIDIYKEVTTDNKIITHGILFDVDKATIRPESMGTINSIYKLL
                     NATPGLKFEIDGHTDNSGNAAHNLTLSQQRADAVRAQLVSMGIDASRLTTKGFGDTKP
                     IGPNDTPENKANNRRVEFIKI"
     gene            11169..13139
                     /locus_tag="VGQ51_05735"
     CDS             11169..13139
                     /locus_tag="VGQ51_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036097.1"
                     /translation="MNRLLFLALCSSIGIGAPAQSDTSHRFLWFTGHWSPTKKNTFVV
                     TGATADGGSDAASSTGGLYAAGATTLTVENDGSVRINSPGIDEWNRWVHKATGSERQF
                     IRQLEEPGDNLDPATHHLYQLILTDAQAEEEEINDLKIDPAQDILSPDTKPSQSTASS
                     QANGSARSSLQQPTDDYCRQVKADYDEVIAWWKAHRHDKDADLTYAPPPGFEYNCYSC
                     DSGLRKVYDATVDNYTRDFFHPEDHLIHKALAIMKAISFRSACSYMSTDDLATALGEI
                     AYHAFRRADKMMMDNRKNFKAEAAIAQTWLKAARDYILITGAKNFANSGIAEIGSIIG
                     KTIEYYTDQLRQNDWRQIGNIPLILNLLHDQAMVGSTDAGFRDLLTRLQKIWNGFKLT
                     IEMDIKVGKNGGYEIEHLKGEGHIIPAFRQDSNQCYKWVMADENRVNNQGFYMASTSQ
                     ALDCNLLDAEVITPADRPKYVGTKFYRTTLKSLSMDFCNPGHDTIMLTNMYPNPATAG
                     QWVYPVGILNLGDNNIDEFFQSIADRKRLTDDGEAKQAAAQLKQQGEQLVAQMQALKS
                     QLGGPQGAANYEKMMEMKNKAHALTTTSVAGKLLYIDFLLPVHNNNPVLVDKRFDAKE
                     INPSENAVIVYGYYTIRIDNDANGRSKTPPTK"
     gene            13136..14056
                     /locus_tag="VGQ51_05740"
     CDS             13136..14056
                     /locus_tag="VGQ51_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036098.1"
                     /translation="MNRTSRLLLLTGLSLTVRLTAMAQCALSPAECPDVHSYRYGDAE
                     DSARRLGNPVLPREIALENRLRRIATGIMDQITTKEHWSYTELTEENSIGYNLADGSI
                     LKYPLRPPHWLTIHFQVVVNDDSLNAWRSWMQDFAQRRLDAMTAAMKGKTDEKTANKQ
                     GEDFDRESQRMTFRYREASIMIVEMEFNSDFARVVGKPTGSAPAAPVSGQILWLNNAN
                     PDLSLADFPGRSHQNAVLLIGTWRRQPSGDYRPAWYFDKKATDEASDKRIKSDELQSI
                     DVRLSGNPAAMKKCIADLPVMELGSLIVQP"
     gene            complement(14061..15092)
                     /locus_tag="VGQ51_05745"
     CDS             complement(14061..15092)
                     /locus_tag="VGQ51_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV9036099.1"
                     /translation="MIRPATIKDIAQRLNISISTVSRSLRNAPDVSPDTKQAVIALSE
                     ELNYQPSKLALSLRQKQTHTIGVIVPNLDYVLSTMVRGIDEVALEAGYTVMVCQSNES
                     YGREILNTRRLLDSLVDGLIISVSSETKLFDHFIKVQDRGLPIVIFDRVTPDLIAPSV
                     RIDNSEGGFLATEHLLEQGYRRIAILAGPANLGISNSRMEGYLKALKRYKIKRDGSLI
                     VYCDFNQDYAYYATRELLAMKKRPDAIFTISDRMAIGAMLAIKEKGLRMPQDIGLVGF
                     NNEPVTGLVTPAISSVDMPSFELGKAAAKLFIETMHNNEHMGKVEEVLRPRLQVRESS
                     MRLKAGRKK"
     gene            15304..18396
                     /locus_tag="VGQ51_05750"
     CDS             15304..18396
                     /locus_tag="VGQ51_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9036100.1"
                     /translation="MKKERLRFLPFTLPFTLLLSFCLHAQQPVVITGTITNSKTGETL
                     EGVSITINKQTTGTTSKTDGTYSIAVTPGSRILSFSFVGFLKQSVDIAGKTKIDIALT
                     PDNSSLNDAVVIGYGTQRKRDLTGSVVSISTKDLAETPITRPDQMLQGRSSGVQVTQT
                     NSAPGGNISIRIRGTNSINSSNEPLFVVDGFPGAGDLNTINPQDIESIEILKDASSTA
                     IYGSRGANGVVLITTRKGHVGAPSVNFESYYGLQKVNKTYQMMDATQFATYLDSVTAQ
                     NNRLTNGTTALPYSAARIGTLGKGTNWEHAVLRTAPIQSFQLSLNGGATNSRYNLSGN
                     FFDQQGIVIGSWFKRGTLRFNFDNTISPKVRMGLASQFAFASQNQALVNTNGGASGGT
                     LYDALRFNPALPIKDSTGAYTYVNGPAPYADLAGNPVAYALETTNKTNNFRSLIDAFL
                     EYEIIRGLKFKALVGADINYTTLDFFTPSTLYLSTQNTSTGNAVKNANTNYSWVNENT
                     FAYDKKINSDNVINAVAGFSWQVFQQGTMTGASNGFFTNDLGTNNLSIGANPLVPGSS
                     QQKNTLASWFGRLNYRLFEKYLFTATMRADASSRFGVGNKWGYFPSGAVAWRLIDENF
                     IRNLNTFSDLKLRIGYGVTGNQEIGNYQSIPQYVTSTANSYTLNNVRVVGVAINNIPN
                     PNLSWEATASTDIGLDFGLLNNRLTGTIDLYSKKTTRLLFNEFVPTTSGFSSELINAG
                     SVGNKGIDIGLDFIAIDRKDFGWKIFGNFSSNRNKVLDLNGTNNLLAGNSSTSIFTGG
                     GQPTTILRVGQPIGSFYGYKFAGIWQNAKQIAASGQKSPVVNPGDPIYVDENGDSSIT
                     GADRVIIGHALPKFIYGFSNNIRWKQFNLSFFIQGVYGDNILNENLYDIQNGFSTDNK
                     LASVAYSWAGDGTSNTLPRVSSTLRRSTGITSDVIQDGSYLRLKTVSLSYNVHISRSG
                     TIKSLLIYVTGQNLLTRTRYSGFDPEVNSYGNSNLSLNTDYGSYPSARSFLLGIKMGL
                     "
     gene            18416..>19070
                     /locus_tag="VGQ51_05755"
     CDS             18416..>19070
                     /locus_tag="VGQ51_05755"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9036101.1"
                     /translation="MKKSVIIILFTCIAVGSCKKFLTETPYSLLSTNNFYQSAGDASV
                     ALNGVFATLTAQTYYGRTSWTVTELGGEDLTVPANSSSDRITLGNYTANSSNGEVANW
                     WNNIYNCINRANDVIANVPKINMDPASRNDIVGNARFLRALGYFELVRSFGSVPLLTT
                     PTSSTSNLFPPKAAIGDIYTQIIGDLQYAETNCLLESQIMAANKGRVSSGAASSILAK
                     "
BASE COUNT         4898 a         5341 c         4772 g         4059 t
ORIGIN      
        1 tgccttcgac acctacaatg atgagcgaaa tgccatggtc ttcgcgacca acgcctacgg
       61 agttcaacgc gatcttctgt cctttgacga tctgtattac gacattgact gggacggact
      121 ctggaatgta cgtaccaacc gaacagactc cggctggact gccgaaatgg ccattccctg
      181 gaagacccta cgctatcccg ggacaaaaga caccacgcaa tcctggggct tcaatgttta
      241 ccggaacaga cgattgacga acgagatcag cgcctttgcg ccctttccac ggatcttctc
      301 cgctacgcac atggattacg cgggccggct caccaatctc cagcctccac caccgtccac
      361 caacataacg ttcgacccat acgttcttac acaggtggat cactattcca attttggaaa
      421 cgcagaaccg tctcacgctt ccacaataaa atttggcggc gacctgaaat gggcattgac
      481 gcctaactcc gtcctggatc tgacggctca taccgatttc gcccaggccg acgtagagca
      541 acaagtgaac aacacttccc gcttcaatgt cttttttcct gaaaagcggt cgttctttct
      601 tgaaaacgca tcccttttcg gcgttcaggt aaacatgaac gtagataatt caggagggtc
      661 aatgcgctat cagcctttta acagccgcac tatcggtctg gattccatcg gaaaccccgt
      721 acccgtcgta gggggcggtc gtttcgtcca ccgctcggca actaccaact atggcgccat
      781 cgccatacgg caggacggcg tcaatggtga ggagcccacc aatttctttg taggtcgttt
      841 ttcccgcaac ttcgccaagc aggatcggat cggcgcgcta gtcaccgtaa aaaaccagtc
      901 cgccggcaca aacatcgagt cgacacttga cggctttttc cgtttgggcg aatccaactc
      961 catcaacgcc atatttacac aaacgacgac ctccaccacc ggtgcaaagg gcttcggcgg
     1021 tatcgtacag tacttcaata cgaataacaa tcacgagatt tggctcacgg agtcattgtt
     1081 cacccgtgac ttcgatcctc agatggggtt tttatcccgc agtgacgtga taggcacgac
     1141 cccggggatg aattggttct atcggggcag gctgctgccg tttaaaaaag tgatgctctc
     1201 gtatgccccc ggtattacgc cggagctgta ttttaccgct tccacccgcc agttctcgga
     1261 agcggatgta cctatttatc ccatttggtt caattttaaa gacggcagct tcatcggtta
     1321 tggcttctca atgatatggc aacgtcttgc ctctgccttt tctcctctcg atataaccat
     1381 cgctcccggt gactaccact acacgaacca ccaggttttt attggaacca atccatccag
     1441 aatggtcagc ggccaattca tgctcaccgc cggaagctat tttaacggcc atcttataac
     1501 agcggattat aaactggcat tcgcccccat cccgaatatt tcgcttcagg gcgaattcaa
     1561 tcggaaccac tttgacaaag taggtatcaa catggcgaca caaaccgtcg acctttacat
     1621 cttacaatgt cgcctcgccc tgaacccacg ccttcagttt accggcattt accaaaagaa
     1681 ttccatcaac agctccgatg cttataattt acgtctggct tgggaatttt cacctttaag
     1741 ctatctttac ctgatatata accggggtat aaataaccag ctcgcacaat caccggttaa
     1801 gagtcaaacg gaagaccatc tgatcttcaa ggcgagctac ctcaaacaat tttgactatg
     1861 caaatatccg acaagtaaaa gagagaatcc gacaattccg cggggaccat cccctgtaaa
     1921 tttaccggta acaacctctt ccaaacctct tcaaattcaa tacgatcagc catgccaaca
     1981 cgaagaaatt tcataaaaca ggccagttta gcaagcgccg gatttgcatt taccaaaaac
     2041 ggcctttccc cgaacaaaca acttatcggg cttcaactgt acacggttcg cgaccagatc
     2101 gctaaagatc cggatggcgt attgggcaag gtggccgcca tcggctacaa ttccgtcgag
     2161 ctaatgggtt atgggaatgg aaagtatttt gggctgacac cggcgcagct cgccgccctg
     2221 cttaaaaagc accgattggt cagccccagc gggcattata tgatgatgaa ctacttcatg
     2281 aagaatgacc ggaatgacct gaaggacaat atcgccgccg ccgtaaccct gggccacaaa
     2341 tttattacga tcccctttct gacggataat atgcgcacca gtctggatga ctataaaagg
     2401 ctggctgcga aattcaacga ggcggctgtg gaagccaaaa aaagcggctt aagacttgct
     2461 taccacaacc atgatttcga gttcaaagac tgggggggcg gcaaaaccgg gttcggaatt
     2521 ctgctaaagg aaaccgatcc ctcgctggtc ttctttgaaa tggacatgta ctgggtggtc
     2581 agggcaggac ataatccaat ccagctgatc agggcgaatc cgggccgttt caaaatgtgg
     2641 catatcaagg acatgtcgca aaaggaaccg gcaagctata cggtcgaggg acaacaatat
     2701 tttgcggaag taggggcggg tatcatcgac ttcaaggaca ttttccgcca taaaaaagaa
     2761 tccggaatgg agtacttctt tgtcgagcag gaccagacca gcgagcccgt tttcgacgca
     2821 attacaaaga gctaccgcta cgtaaagacg aatcttgcgt aataacaaat cctgaagaca
     2881 ggtcataaac taaaattttt aatccaatac aatggccgat aatcattttg atgtgatcgt
     2941 cgtaggctcg ggcatcagcg gtggctgggc ggcaaaagag cttaccgaaa aagggctgac
     3001 ggtgctgctg ctcgaacggg ggcgaaacat cgaacacgta aaagactatg tgaatgcgac
     3061 taaggcgccc tgggattttc cacaccgggg ctccaaaacg cagcagatga tcaagaatta
     3121 tccggtcctc aaccgggact atcccctcaa cgagactaat ctcgacttct ggactaacga
     3181 gcaggaatcg ccgttcacgg agatcaaacg gttcgactgg ttccgcggct accatgttgg
     3241 cggccggtcg ctgatgtggg gccgacaaag ctatcgctgg agcgatctcc agttcgaggc
     3301 taacgccagg gaaggcatcg gcgttgactg gccggtgcgt tacgcggaca tcgcgccctg
     3361 gtacgaccat gttgaaaaat ttgccgggat cagcggctcg aaggagggtc tgccacagct
     3421 gccggacggc cggtttatgc ccgcgatgga tatgaatatc gtggagaaag acctggctgc
     3481 ccgcttcaac gatcattata aggatcaccg gaagatgatc atcggccgta cggccaatat
     3541 caccgaacca ctgccgggtc gcaccaactg ccaataccgt gataagtgct ggctcggttg
     3601 cccattcggc gcctatttca gcacccaaag ctctacccta cccgccgcca tggccaccgg
     3661 taagctgaca ctgcggccgt gggcgatcgt tacacgtgtc ctttatgaca aggataaaaa
     3721 acgtgcgacg ggcgtcgaga tcatggatgc agagaccaac aagacgtaca cgtatacttc
     3781 gaagatcgtg ttcctaaacg cctcgacgat aaataccgcc tggctactca tgaactcggc
     3841 tacagacgta tggcccgacg gtctgggatc cagcagcgga caactggggc acaaccttat
     3901 ggaccatcat ctgggtgtgg gcgcattcgg cagggtcgaa ggatatgaag acaaatatta
     3961 ctacggtcgc cgggcgaacg gcatctatat cccgcatttc cggaatcttt tcggcgacaa
     4021 gcgcgactat atccgaggtt ttggctatca gggcggcgcg ggccgggacg gctgggatcg
     4081 cagcattgcc gagctgagca tcggttccga cctgagggaa gcactatcag agcctggcaa
     4141 ctggaccttc gggataggag gcttcggcga gaccttgccc tatcatgaga acaaagtgac
     4201 gctcgacaag acccggaaag acaaatgggg tttgaacatt cctgctatcg acgctgtgtt
     4261 gcgggacaac gagctcaaga tgcgcaaaga tatgcagggc gacgctgttg agatgcttac
     4321 cacggtaggc gtcaaagacg ttcagggttt cgaaggcaat gcggtgctcg gccgcggcat
     4381 ccatgagatg gggacctgcc ggatgggtaa ggatccaaag acttcagtgc tgaacaaatg
     4441 gaaccaggtc tgggacgcgc cgaatgtata tgtcaccgac ggatctttta tgacgtcggc
     4501 caattgctgc aatccgtcgc ttacctatat ggcctttacg gcaagggcgg caaatcatgc
     4561 tgtcgatgaa ctcaaaaaaa tgaacctcta aaagctaaag ctatgcaaag aagggaagca
     4621 ttacaacggg ttgccgtcct tgtaggggga actgtgatcg gcgccgaagc ctttttgtcg
     4681 ggctgtaaga ctcctccgcg aaaagaaggt cttttttacg ataccgatgt cgcgctgatg
     4741 ggtgagatcg gtgaaacgat ccttccggca ggcggtggcg tccccggggc caaagaggct
     4801 ggcatcggcg agtttatgaa ggtgatcgta actgattgct atacccccgg ggaccaggat
     4861 gtttttgtga aaggcttgca gacgctcagg gataaaaaat ttatggacct cagcgccggt
     4921 cagcggcatg acctgctcgt ggaactcgat aaggaagctc gcgatccgaa atacaacgac
     4981 aagaacaagc actatttcaa aatgctgaag caactgacca tctggggata tttctcctcc
     5041 gaggcggggg cgacgaaggc aacccgctat atcgagacgc cggggcatta cgatggaaac
     5101 ctcccttata agaaagggga caaagcctgg gccacttaac gcatcctgaa agacccggca
     5161 tggttttttt atagccttta aaggggaatt ccatccccat taccgttagc tcaaaataaa
     5221 ggtatgatgg caaaaaaatc aattctcagt tatggtctga tccccctgac tttcttattc
     5281 cttggtcaac ctgccaatgg ccaatccccc gactcttccg gtagccccag gcgttaccat
     5341 gtcaattatg tcactggcag catcatcatc ggcattggtc tgggcaccga cgcttttgcc
     5401 atcagccgga tccaggataa gccaaacctg accgccgccg agctgcaagc gctgaatccc
     5461 ggtgtcctta attcttttga caggtgggca ctgcaccagg atccaagcgg atataagcat
     5521 tacagtaaaa tttccgatgt catcgaaccg cccattttta tcctgttacc cgccttgctc
     5581 gccctggata aaaaaatccc aaaaaaggaa tggatggaca tcctcttcat gtatatcgaa
     5641 gggcatacca tcacctttac cttttacaat tatagctggc tgggccccac ctttcaaaac
     5701 aggttccggc cccttaccta ttattcacag ctgccgcttg ccgatcgcat gagcggaggt
     5761 aaccgaaact cattctatag tggtcatacc gcatccgtcg ccttcactac tttttttatt
     5821 gccaaggtat tttgtgacta tcatcccgat ctgggcttcg gtaaatatct cctgtatacg
     5881 ggtgccctca tcccaccggt ggttatgggc tattttcgcg tgaaggcgct ggcgcatttt
     5941 ccttccgacg atctggtcgg tcttaccctg ggggcagtgg tcggcatagt acttccagcc
     6001 ttgcacaagg ccaactataa aggcgtcgct atcaaccttt attccaatcc cgagtctacc
     6061 gggctgaatg tatgttggac aatgccggat cacaagaagt aataccggct acgggcgact
     6121 tagacggaag cgtacaggag ttttcgaaaa tttccgtatt ttatcgcaat aaaaccctgg
     6181 aatgcgtttt ctcctcctga tcatcctgac ccttggctgt acagcggcgg catcgccaca
     6241 aatccccacc tcattcgaac agccggacgt acttgtcaag gcaggcgtaa aacgtgtgaa
     6301 atgtatcctc aaagacagcc ggggattttt gtggatcggg accgagaccg ggctcttccg
     6361 gtttgacggc accaaccttg acctgatgca acacgaagca gacagcgcgc ttggcttgcc
     6421 aaatagcagg atcgcgggac tcgcggaaga cacatcgggt aatatttggg tggggacgat
     6481 gcatggcgcg gcacggctcg acccctggac gctggtatgt accgtataca cgccgcagca
     6541 gaagaacatc gatcttgatt tcgacgtgaa gccgatgatc agtaatgatg gaagggtctg
     6601 gcttggcggc aacgacggtc tctgcgttct ggaaggtggc cggttccgga cggtgacgca
     6661 cgccgaagcg aacgctcccc ccaacgcatg gtatgtgaac tcgatgacgc aatggaaagg
     6721 cgacaccatg gccgtcggaa catttgatgg cctggtgctt tataacatgc ggaacggcgg
     6781 ctttacgaga ttgttcggcg gccggcacat gacggtaacc aagcttcatg tcgatcgacg
     6841 cagccggctg tggatgggca catggggcgg aggctgcttc atgctggaca gcaacggcag
     6901 gagttatagc agactcgccc ccgacaaacc ggtccacggg gaattcagga acatcatcac
     6961 gggtatcctt gaaacggaca gtcgcgaggc gccgcatttt tggatctcca ccgaggcagg
     7021 tgtctaccag gtggaagatc ctgccgaaca gcaagaagga aaacctgaat gccggcagat
     7081 cgccgacaaa ggcgcagcgt gcatcgccgg cgacaatgcc cgttatatct ggatcggtgg
     7141 ccagacggtg acgagggtct ttgccgggca cagcgggttc gggatccttc cggcgacaat
     7201 aaagggttcg gtagccgcta tacagcccgt aacctatgac ggtcagcctg ccgtcgcttt
     7261 ctctacctgg tatgtctccg gtggactaac ggtgaccaac ccgtcaggaa caaccaccta
     7321 ctaccggcgc aaggcccggg gggatgcgga aaacgtctcg ggcctggcca cggatagcct
     7381 tggccgccac tgggtatcga cattcgacgg attggatatc ctcgaccaac gctttatgcc
     7441 cgtacgcggc gccgacacct tattcagggc gcgcgacaag ctggtcacaa aaaggacata
     7501 cggcgttctg atccatcgcg ataccgcctg ggtggcctac taccggcatg gtttatccct
     7561 gtatgacatg cattttcatc tgctgcacag cttttcgaaa aatgacgggt cggggctggc
     7621 ggatgacctg atcaaccgca tggtcgccga taaggtggga cgggtatggc tgggcggagg
     7681 caaaggcctg tacgaatacg atgccgcggc agggaggttc aggctgttta atcttaatcc
     7741 ggacggatca cctttcgaag tcagcgatat cgccacgctg ccgggcggcg acctcatcct
     7801 gtccacgacc tcaggcattt ttcgcttttc gcccgccact ggcgccagtt cgaagatacg
     7861 gtcgccatta ttcccggata atgttatcga gacagcagct gtcgataagg gtggcgatat
     7921 ctggttcatg aaccgcgagc acctcgtcta ttaccaggta aacagcggac attttaccct
     7981 tttcgggacg gaggacggac tagatacgcg acaggacctc agcacgctgc gatgccccga
     8041 tggcgaacat ttttatctcg ccggtgacca tctcatctat accttcaacc gaaacatccg
     8101 gaatatcgcc gcgcagcctg tcccgctcta cctgcattcc atccaggtcg acgacagtac
     8161 gcttccacgt aatgccggag tcccgccgtt gcacctcaac tacaaacaag atcgtattac
     8221 tattgaattc ggcgccatca acctggtcaa gcccgaacaa aacctgtatg cctatatgct
     8281 aaccggtgtc gacactaggt ggatctatag cacccgcaac tatgcgtcgt atgctaacct
     8341 cgcacccgga ggctatactt ttcgtttaaa ggtagagaac gatgcgggaa catggagcga
     8401 tgtcctcagc cttccggtcg cgatccaccc cccttactgg gcgacatggt ggttcaggct
     8461 gctaggtctg acactgatcg gggtggcagt ggtcttgtca gtccgatatg tcgtccaacg
     8521 caatctgcgc gaacgcatcc tccggctaca acgcgaacaa gcggtggaga aggaacgcaa
     8581 ccgtattgca cgggatatgc atgacgacct gggaagcggt ctgacgaaaa ttgcgatcat
     8641 gagcgaggtc accaaggcgc agctggccgt tccgggtgcc gcagtaacca atctcgacgt
     8701 tatatccaat gccagcaggg aactggtgga caacctgcag gatattgttt gggtgctaaa
     8761 cccgcgtaat gattcgattg gcagcctgct tttgtacctc aaggaatatg tcgaggattt
     8821 cttcgagccg accgggatat cctgtacggt ggtcgccaat acggttgcac acgatattcc
     8881 catctcggaa gagaagcggc gcaacatctt cctgacggtg aaggaggcct gcaataacat
     8941 tttgaagtat gcggaatgca aggaggtcgg gctggccgta cggatcgatt cccgccggtt
     9001 gaccataaag ctcgaagata acggcaacgg tttcgacata cgggaagtgg gaccattcgc
     9061 caatggattg aagaacatgc ggaaccggat agaacagatc ggtggacggt ttcagatgga
     9121 gtcggaaaag gagatcggta cgatcgtcaa catagaggtg ccggtatgag gggcgtgacg
     9181 ggcctaaccc ttttttgcta ttgtcggaat gccccgaagc aataaattca cacgcaatta
     9241 tgatccaggt cgcgatagtc gaggacatac gggaaatccg ggaagggttg caaatgctga
     9301 tcaatttcag cgacagcttt aactgttcgc aggtgtatgc gagtggagaa gacgctttaa
     9361 aggggttacc gacggaaaac ccggacgtag tactgatgga tatcaatctt cccggtatga
     9421 ccggcgtcga atgcgtacgg cgcgtgaaac ccctctgccc tgccacccaa ttcatcatga
     9481 gcaccgtata cgaggacgac gagaatgtct tcgaatcgct gaaggccagc gcgaccggtt
     9541 atctcttgaa aaagaccgta cccgcgcgga tactcgaggc gattgccgag gtacaccagg
     9601 gaggttcgcc gatgagtacg cagatagccc ggcgaattat cgtgaccttt cagaaaaagg
     9661 acggcatcgc ggctgcagat gagcttacgt cgagggaaaa ggagatcctc aagagccttt
     9721 cggaaggctt acggtataaa gagatcgcgg cccggttccg gatcagtatg gatacggtcc
     9781 gcagccatgc gcggaatatt tacgaaaaac tccaggtgca aagtcggaca gaagcgctga
     9841 acaaggtcta tggaaaagac cggcgggtct aacacttttt tgctatggcc ggggccatga
     9901 ctgagcatta gatttacgcc aaaaacagcc atgaaaaaga ccctgtcggt tctattcgcg
     9961 ctgctcatcg cagtcgcatt acaggctcaa gtggagaacc ccggaactgc cgccaagaat
    10021 ggagcgaccg atcacgccaa caacgatatc aataattcga tcaataacgg cctcaacaag
    10081 accgaaggcg cgatcaaggg tcttttcaag aagaaaaaga aagacgccgc ggcaacccag
    10141 cctcccgcgt caacccaatc cgccatccct acccaacctg ctattgcgcc cggtaccgcg
    10201 tcccccaccg atagcaccca gcccggccag ggcctcgcag tctacaaaaa ttacgatttc
    10261 gtaccgggtg acaagatcat cttccagtcg gcgctttccg acgaatccgt cggcgagata
    10321 ccttcccaac tcaccctgaa caaaggacaa atggacatcc aacaggaaga cggcgaaaat
    10381 gtgatccata ttcccacggg cccgggcgcg aatttcagcc cgcgcatgaa gaacatgcag
    10441 tatatgccgg accagtttac gatcgagttc gatgtaaaga acgaaaggtt cggactagac
    10501 cattttagta tctatttcgg cgactacaga cacgacgatg gctcgctcca gatactccgc
    10561 atcaccgacg cgtcgatgac ttggacagcg ggaaacgtag aagctccccg gggactcgac
    10621 ctcaagaaac cgatgacctg gcatcacatc gctattgcga tcaataaaaa tgtcggaaag
    10681 gtgtacgtcg atcaattccg tttcatcagt atcaacaacc tggaaggaaa agccaaccag
    10741 atcactttcg atgtcaacgg ctacgaaaac agcttcataa agaacgtccg gatcgccgcc
    10801 ggcggcattg atatctataa agaagtcacc accgacaata agatcatcac acacggcatc
    10861 ctgttcgatg ttgacaaggc aaccatccgt cccgagtcga tggggacgat caatagtatt
    10921 tacaaactgc tgaatgcaac gccgggttta aagttcgaga tcgacggcca tacggacaat
    10981 agcggcaatg ccgcacataa cctgacactg tcgcaacaaa gagccgacgc cgtcagggcc
    11041 caactggtat ccatgggtat cgatgcttcc cggctcacga caaaagggtt cggcgatacc
    11101 aagcccatcg gtccgaacga tacacccgaa aataaggcca acaaccgccg cgtcgaattc
    11161 ataaaaatat gaaccgcctt ctctttctgg ccctctgcag ctcgatcggt atcggtgccc
    11221 ccgcgcagtc cgataccagc caccgattcc tatggttcac cggccactgg agcccgacga
    11281 aaaaaaatac tttcgtggtg accggcgcta ctgccgatgg gggcagcgac gccgcgtctt
    11341 caacgggcgg attgtatgcc gctggcgcca cgacccttac tgtcgaaaac gacggctcgg
    11401 tccgcatcaa cagccccggc atcgatgaat ggaacaggtg ggtccacaaa gccaccgggt
    11461 ccgaacgaca gtttatccgc cagctcgaag aaccgggaga caacctcgat ccggctaccc
    11521 atcatttata ccagctgatc ctgaccgatg cacaggcaga agaagaggag atcaacgacc
    11581 tcaagatcga tcccgcacaa gacatcctca gcccggatac taaacccagc cagtccaccg
    11641 catccagcca ggcaaacggg tctgcccggt cctccctaca acagcccacc gacgactact
    11701 gtcgacaggt taaggccgac tatgacgagg tgatcgcctg gtggaaagcc caccgccatg
    11761 acaaagacgc cgaccttacc tatgctccgc cgccgggatt cgaatacaat tgttactctt
    11821 gcgattccgg tctacgaaag gtctatgacg caaccgtcga taattatact cgtgacttct
    11881 tccatccgga agaccacctg atccataaag ccctcgctat catgaaagcg atcagcttcc
    11941 gctctgcatg ctcctatatg tccacggatg accttgcaac agccctcggc gaaatcgcct
    12001 accatgcctt tcggcgggcg gataaaatga tgatggacaa cagaaaaaac ttcaaagcgg
    12061 aggccgcgat cgcacaaaca tggctgaaag ccgcccgaga ctacatcctt atcaccggag
    12121 ctaaaaactt tgctaacagt ggcatagccg agatcggcag tatcatcgga aaaacaatag
    12181 aatattacac ggatcagctt cggcagaacg actggcgaca gataggtaac attccgctta
    12241 ttttaaacct cctgcatgac caggccatgg tgggaagcac tgacgccggt tttcgggacc
    12301 tcctcactcg cctgcagaaa atttggaacg gattcaagct gacgatcgaa atggatatca
    12361 aagtcggaaa aaatggcggc tacgagatcg aacatctgaa aggagaagga catatcatcc
    12421 cggcattccg gcaggacagc aaccaatgct acaaatgggt gatggcagac gagaacaggg
    12481 taaacaatca gggtttctat atggcttcga cctcacaggc tctcgattgc aacctgctcg
    12541 acgccgaagt gatcactccg gcggacagac cgaaatatgt cggaaccaaa ttctatcgca
    12601 caaccctcaa atcgctcagc atggatttct gtaaccccgg ccatgacacg atcatgctga
    12661 ccaatatgta ccccaatcca gcgacagccg gtcaatgggt ttaccccgtc ggtatcctca
    12721 acctcggcga taataacatc gacgagttct tccaaagcat cgcagaccga aaaaggctga
    12781 ccgacgacgg agaagcaaag caggccgctg cacagctcaa acaacaaggc gaacagctcg
    12841 tagcgcaaat gcaggccctg aagtcgcagc tgggcggtcc acagggtgcg gcgaattatg
    12901 aaaaaatgat ggagatgaag aacaaagccc acgcactcac caccacatcg gtagccggca
    12961 agttgctata tatcgacttt cttctgccgg tccacaacaa caaccccgtg ctcgtcgaca
    13021 agcgcttcga tgcgaaggag atcaacccct cggaaaatgc cgtcatcgtt tatggctatt
    13081 ataccatccg catcgacaat gacgctaacg ggagatcaaa aaccccaccc acgaaatgaa
    13141 tcgaactagc cgcctcctcc tgttaaccgg actatctctt accgtccgcc tcaccgccat
    13201 ggcccaatgc gccctttctc cggccgaatg cccggatgtc cactcctatc ggtatggaga
    13261 cgcggaagat agcgccaggc gactgggcaa tccggtgctg ccacgcgaga tcgcgctgga
    13321 aaaccggctg cgccggatcg ccaccggcat catggatcag atcacaacga aggaacactg
    13381 gtcatacacc gagctgaccg aggagaacag catcggctat aaccttgccg acggttcgat
    13441 cctgaaatat ccattacgcc ccccacactg gttgacgatc cattttcagg tggtggtcaa
    13501 cgacgattca ctcaatgcct ggcgttcatg gatgcaagat ttcgcacaac ggcggctgga
    13561 tgccatgacg gccgctatga aagggaagac cgatgaaaaa accgccaata aacaaggcga
    13621 ggactttgac agagagtcgc aacgcatgac tttccgatac cgcgaagcct cgatcatgat
    13681 cgtcgagatg gaattcaatt cggattttgc gcgcgtggtt ggcaagccca ccggaagcgc
    13741 cccggccgca cccgtttccg gacagatcct atggctgaac aatgccaacc cggatctatc
    13801 gctcgccgat tttccgggcc gttcgcacca gaatgccgtg ctgctcatcg gcacctggcg
    13861 acgccaacca agtggggact atcgaccggc ctggtacttt gacaaaaaag caacggacga
    13921 agcctccgac aaacggatca aatcagatga gctccaatcc atcgatgtcc gtctgtccgg
    13981 taacccggcc gcgatgaaaa aatgtattgc cgatttgccc gttatggaat tgggttcgct
    14041 gatcgttcaa ccgtaatatc ctatttcttc ctccccgctt tcaaccgcat cgacgattcc
    14101 cttacctgca accttggccg caacacctcc tcaactttgc ccatgtgctc attgttatgc
    14161 atcgtctcta tgaacagctt cgcggccgcc tttcccaatt cgaaggacgg catatcgacg
    14221 ctcgagatcg ccggagtaac caaaccggtc accggctcat tgttaaaccc gaccaacccg
    14281 atgtcctgcg gcatcctaag ccccttctcc ttgatggcca gcatggcccc gatagccatc
    14341 cggtcactga tcgtaaatat cgcatccggc ctttttttca tggcaagcaa ttcccgcgtg
    14401 gcataatagg catagtcctg gttgaaatca caatagacga tcagactgcc gtccctcttt
    14461 atcttatatc gcttcaatgc cttcagatac ccttccatgc ggctgttgct gatccctaaa
    14521 ttggcggggc cggccaaaat cgctatccgc cgatacccct gttccagtaa atgctcggtc
    14581 gccaggaacc cgccttctga attgtcgatc cgcacgctcg gagcgatcag gtcgggggtg
    14641 acgcgatcaa agatcacgat cggcaaaccc ctgtcctgca cctttataaa atggtcgaaa
    14701 agcttcgtct cgctcgatac cgatatgatc aggccatcca ccaggctgtc gagcagcctt
    14761 ctcgtgttca gtatctccct accataggat tcattgctct gacagaccat cacggtataa
    14821 ccggcttcca gcgctacttc atcgattccc cttaccatgg tggataacac ataatccagg
    14881 ttcggaacga tgactccaat ggtatgcgtc tgcttttgcc gcaggctgag ggctagttta
    14941 ctgggttgat agtttagttc ttcggacaat gcgatcacag cctgttttgt gtccggactc
    15001 acatccggtg cattacgcag cgatctcgat acggtggaaa tggagatatt caatcgctgc
    15061 gcgatgtctt tgatggtagc aggcctgatc ataaaaagta aagctaagga taatcgctca
    15121 tgagaatccc ggaaccgtta ccggaaacgt tgccgctcca ttttttttaa attttttcta
    15181 acaaaagagc gcatccatca aaatatatcc agccatccct ccttttttta gataaaaaaa
    15241 tccggcacca taaccgattc ccgtcccata agagataata ttgaccgact aaaaactgcc
    15301 agtatgaaaa aagaacgatt gcgtttcctg ccattcacgc ttccattcac cctcctgctc
    15361 tccttttgtc ttcatgccca gcaacccgtc gtcatcaccg gcaccataac gaatagcaag
    15421 accggagaaa cactggaagg cgtaagcatc accatcaaca agcagacgac aggcaccacc
    15481 agcaaaacag acggcaccta ttccatagcg gttacccccg gaagcaggat cctctccttt
    15541 tcctttgtcg gctttctcaa acagtcggtg gatatagccg gaaaaacgaa gatcgatatc
    15601 gcactgacac cggacaacag cagcctgaat gatgcggtgg tcatcggcta tggcacccaa
    15661 cgcaaacggg atctcaccgg atcggtcgta tccatttcga caaaagacct ggcggaaacc
    15721 ccgatcaccc gccccgatca aatgctacag ggcagatcga gcggcgtcca ggtgacccaa
    15781 accaattcgg cgccgggcgg caacatcagt atccgcatcc gcggcacgaa ttccatcaat
    15841 tccagcaacg agccgctctt tgtcgtggac ggctttcccg gcgcgggcga tctcaacacg
    15901 atcaatccgc aggacatcga gtcgatcgag atcctgaagg acgcctcttc caccgccata
    15961 tatggtagcc gcggcgcgaa cggcgttgta ctcatcacca cgcggaaagg tcatgtcgga
    16021 gcgccatccg tcaatttcga aagttattat gggcttcaaa aggtcaacaa gacctatcaa
    16081 atgatggacg ctacccaatt cgctacctat ctcgattcgg tcacggcgca gaacaaccgc
    16141 cttacaaatg gcacgacggc cctgccgtat tccgctgccc ggatcggtac gctgggcaaa
    16201 gggacgaatt gggaacatgc ggtcctgcgg acggcgccga tccaaagctt tcaactgtcc
    16261 ctcaacggcg gcgccaccaa cagccggtat aatctaagcg ggaatttttt cgaccagcaa
    16321 ggcatcgtga tcggttcctg gtttaaaaga gggacgctgc gcttcaattt cgacaacacc
    16381 atcagtccaa aggtgcgcat gggtcttgct tcacagttcg cctttgccag ccagaaccag
    16441 gccctggtca atacgaatgg aggagcctcc ggtggcactc tatacgatgc gctgcgcttc
    16501 aaccccgccc tccctattaa agacagcacg ggcgcctata cctatgtcaa cggccctgcc
    16561 ccatacgccg atcttgccgg caacccggtc gcatatgcgc tggaaacaac caacaaaacg
    16621 aataatttcc gttcattgat cgacgcgttc ctcgaatatg agatcatcag gggtttgaaa
    16681 tttaaggccc tcgtaggcgc ggatattaat tataccactt tggatttctt taccccttcc
    16741 accctgtacc tgagcacgca gaatacctcc acgggcaacg ccgtcaaaaa cgccaatacc
    16801 aattacagct gggtcaacga aaatacattc gcctatgaca agaagatcaa tagcgacaat
    16861 gtcatcaatg cagtagccgg tttttcctgg caggtcttcc agcagggaac gatgacgggc
    16921 gcgtcgaacg ggttctttac caatgacctg ggaacaaaca atctgagcat cggcgccaat
    16981 ccgctcgtgc ccggcagcag ccagcaaaaa aatacgctgg cttcctggtt cggacggttg
    17041 aattaccggc tgtttgaaaa atacctcttt accgccacca tgcgcgccga cgcgtcgtcc
    17101 cgtttcggtg tcggaaacaa atggggctac ttcccttccg gagctgtcgc ctggcgtctc
    17161 atcgacgaaa actttatccg gaacctgaac accttcagcg atctgaaatt aaggatcggc
    17221 tacggcgtaa ccgggaacca ggagatcgga aattatcaat ccattcccca gtacgtcacc
    17281 agcacggcca atagctatac cctcaacaat gtccgggtgg tgggggtcgc gatcaacaat
    17341 atccctaacc ccaatctgag ctgggaagcc acggcttcaa ccgatatcgg tctcgatttc
    17401 ggtcttctga acaaccggtt gaccggtacc atcgacctct atagcaagaa aacaacccgg
    17461 ttattattta acgaattcgt accgacgacc tccggtttct ccagtgagct gatcaatgcg
    17521 ggaagcgtcg gcaataaagg gatcgacatc ggtctcgact tcatcgccat cgaccggaag
    17581 gatttcggct ggaagatctt tggcaacttt tcttccaaca ggaacaaggt gctggacctg
    17641 aatggaacca acaatctgct ggcgggcaac tccagtacga gcatctttac cgggggcggg
    17701 cagccaacaa ccattttaag ggtcggacag ccgatcggct ctttctacgg atataaattt
    17761 gccggcatct ggcaaaacgc caaacagatc gcggcctccg gccaaaaaag cccggtggtg
    17821 aatccgggcg accccatcta tgtcgatgag aatggcgata gcagcatcac cggcgcggat
    17881 agggtgatca tcggtcacgc cctgccgaaa ttcatctatg gctttagcaa taatataaga
    17941 tggaagcaat tcaatttatc cttttttata cagggagtgt acggagacaa tattttaaat
    18001 gagaatctct atgatatcca gaacgggttt tccaccgata ataaactggc gtctgtcgca
    18061 tacagctggg ccggtgacgg aaccagcaat accctgccaa gggtgagcag cacgctgaga
    18121 aggagtaccg gcatcaccag cgacgtcatc caggacggca gctacctccg tctgaagacc
    18181 gtgtcgcttt cctataatgt ccacatatcg cgatccggga cgatcaaatc cctgctgatc
    18241 tacgtcaccg ggcagaacct cctaacacgg acccgctatt ccgggttcga ccccgaagtt
    18301 aattcgtatg gaaacagcaa tctcagtctg aacacggact acggctccta tccttcggca
    18361 aggtcattcc tgctgggaat aaaaatggga ctttaacgca ccaaacaaaa aaatcatgaa
    18421 aaagagcgtc atcataatcc tcttcacgtg catagcagtc ggctcctgta agaaattcct
    18481 gacggaaacg ccctatagcc ttctaagcac gaacaatttt taccagtccg ccggcgacgc
    18541 gtccgtcgca cttaacggcg tctttgccac cctgacagcc caaacctatt acggtaggac
    18601 atcctggacc gttaccgagc tcgggggcga ggatctgacg gttccggcaa acagctcttc
    18661 cgaccggatc acgctgggca attacactgc caatagctcc aacggcgagg tcgccaactg
    18721 gtggaacaac atctataact gtatcaatcg ggccaatgat gtcattgcca atgttccgaa
    18781 gatcaatatg gaccccgcca gccggaacga catcgtaggg aatgcccgct tcctccgcgc
    18841 gctgggttat ttcgagctcg ttaggagttt cggatcagtt ccgttactga cgacgccgac
    18901 cagctcaact tcgaatctct tcccgccgaa ggctgccatc ggggatatct acacgcagat
    18961 catcggcgat ctgcaatatg cggaaaccaa ttgcctgctg gaaagccaga tcatggcggc
    19021 caataaagga cgcgtttcgt caggagccgc ttcctccatc ttagccaagg
//
LOCUS       DASYSV010000189         6690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1314882, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000189 DASYSV010000000
VERSION     DASYSV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314882"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1087
                     /locus_tag="VGQ51_05760"
     CDS             155..1087
                     /locus_tag="VGQ51_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9036102.1"
                     /translation="MPVSYPIQKIGLTPVSQLRTLTEHLRTFNLKNCELVIFECYEPS
                     TEIPLTFADFVITSMVMGRKVMHLDGLPSFEYLPGETVIIPANRSMTIDFPEASFTTP
                     TQCVALKIDTPYIQETLNYLNEKYNTTIDEPNEWKLLIDQYHFNNDAAITDLINKLVR
                     ICSGPENTKDIYADLTLRELLVRIAQNQHLQHQVNGSAQNSNNSRLHFVLQYIHDHLT
                     ERIDVDTLSRKAYLSRNIFFKWFKEQFGITPLEYITRERIRLARELLAHEKYTITQVS
                     LQCGFTDVNYFVRTFKKSEGLTPGAYRDILRKLP"
     gene            complement(1157..1447)
                     /locus_tag="VGQ51_05765"
     CDS             complement(1157..1447)
                     /locus_tag="VGQ51_05765"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV9036103.1"
                     /translation="MEKKYFFVRLNPSRPDFAQTMTPEEFSVMRAHSNYWREWQARGY
                     VVVFGPVLDPAGTFGMGVVGVEEEEEIRKMIEGDPAQALTRIEYFPMRAVLG"
     gene            1446..2387
                     /locus_tag="VGQ51_05770"
     CDS             1446..2387
                     /locus_tag="VGQ51_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV9036104.1"
                     /translation="MEVGLVRLKIGFFHSAPTAEPPAPPASSSRPPATGTPSLILAPA
                     GPPLTPAASPCARGTVFYGVHPMTHTENAPTPAHNFYREMLVQLQATGDPFLVGGGFA
                     FSHYTGIRRDTKDLDIFCKPSQYPFFLQLFDRLGYRTESTDVRWLAKVFKDDHFMDII
                     FDSPNSICQVDDSWYLHGKDAEFEGVAVRMVPAEELIWSKTYVQNRERFDGADINHLL
                     LAQGKQLDWDRLLFRLDRHWHLLLAELLVFQFVYPSEYRDIIPRGLMDMLIRRVGQQY
                     EIPPSVEKLCRGPLIDQTQYATDIRDWQYKVTTIKTV"
     gene            2398..3138
                     /locus_tag="VGQ51_05775"
     CDS             2398..3138
                     /locus_tag="VGQ51_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV9036105.1"
                     /translation="MSTPEGKTRIAAIADIHVRETDRNSLAGLFREVSHQADILLIAG
                     DLTNTGDEAEAEVLKEELKTCSIPVIAVLGNHDHEKGRQKTIRQILQNDQVHILDGEA
                     FAIGNVGFAGIKGFGGGFDNYMLSMFGESAMKAFVQEAVNDTLSLERALARLDQQFDG
                     LKKIALLHYSPTHDTVKGEPEQIYPFLGSSRLAEPLIRRNVTAVFHGHAHAGTLEGQL
                     PGGIRVFNVARPVLLKAGLPSPFFLLEV"
     gene            3579..3773
                     /locus_tag="VGQ51_05780"
     CDS             3579..3773
                     /locus_tag="VGQ51_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036106.1"
                     /translation="MKNSIKKIVGMMLLAGLLITGFTSCYETTYYHHYHHHTHDWYGH
                     HHQEVPVGVDFNVDVEAHHH"
     gene            complement(3861..5912)
                     /locus_tag="VGQ51_05785"
     CDS             complement(3861..5912)
                     /locus_tag="VGQ51_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HEV9036107.1"
                     /translation="MKTMLCFILVAACGVAMAQPAAGQRDYPISPVPFTAVTVNDGFW
                     LPRIETNRTVTIPASFERCRTTGRVHNFEMAAARTGKFCTTYPFDDTDIYKTIEGASY
                     SLAVHPDPALAAYLDSLIGLIGRAQEADGYLYTARTIDPQHVHPWAGPERWMHEAELS
                     HELYCSGHLFEAAVAHYNATGRRNFLDIALKNADLLVRTFGPDKKHVAPGHEVVEMGL
                     VKLYRVTGRKEYLDLAKFFLEMRGERQYDKTSTDVWKNGRYWQAHEPVTAQDEVVGHA
                     VRAMYLYSAMTDVAALTGDSTYVRAVDRLWDNMVGKKMYLHGGIGAIGDGERFGDNYE
                     LPNATAYAETCAAVGSVFWDYRMFLLHGDAKYIDVMERTLYNGLLSGVGLDGKSFFYT
                     NAMEVVNGHPYPDLEPERAGWFTCSCCPTNLARFLPSVPGYVYAVRGRDVYVNLYVSG
                     KAKIELGAVETAKAAAGMQAIGLEQQSNYPLNGDIRLRVEVGRPMPFRLLLRIPGWAR
                     GEAIPGGLYRFAGEATERVMVKVNGQVVDAPMQQGYAVIDRKWKNGDSVELVLPMKPQ
                     MVEACDSVKADIGKVALQCGPLVYCAEWADNSGRTSNLILPVKSGFSLRAGKVLAGVN
                     MISAAGVRVVVGEDGLNVSTRDAPIELIPYYAWANRGKGEMTLWFPEKVKEVELVSRK
                     E"
     gene            5967..>6690
                     /locus_tag="VGQ51_05790"
     CDS             5967..>6690
                     /locus_tag="VGQ51_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV9036108.1"
                     /translation="MGVLFQTFYWNCPSLENQAYQWFNYVKPRITSLAATGFTAIWLP
                     PFCKAACIGGPSMGYDPYDYWDLGELDQKGGIPTWFGSRADLLALIAEAHTHNIDVYA
                     DLVFNHNSGADAEELNPVTNSMGWTKFTPASGKFPRDWECFHPSEYETTDGFSFGGLP
                     DLCHRNPDVYTSLLEYTRYLLGTIGIDGFRYDCVKGYGGWMVRAIQELRGLRNDIAYR
                     PFGVGECWDTDRTILDWMTEANA"
BASE COUNT         1531 a         2293 c         1418 g         1448 t
ORIGIN      
        1 gtgctaatat gcctgtttat gtgggctact acatgaatta tcgtatttat taatagcaca
       61 atcgtacctc tctttgttat cgccggatca atgatcatga aggaatactg cagttttttc
      121 attaacttta aagtgcactc ataacctgtt gcccatgcca gtaagttacc caattcagaa
      181 aattgggctc acccctgtat cccagttgcg gacgctcacc gagcacctcc gcaccttcaa
      241 tctgaagaat tgcgaattag tcatttttga atgttatgag cccagcacgg aaatccctct
      301 cacgttcgcc gatttcgtca tcacgagcat ggtgatgggc agaaaggtca tgcatctcga
      361 cggccttccc agcttcgaat accttccggg ggagacggta attatcccgg ccaaccggtc
      421 catgaccatc gattttccgg aagccagctt caccactccc acacaatgcg tcgccctgaa
      481 gatcgatacc ccgtatatac aggaaacatt gaactatctg aatgagaaat ataacactac
      541 gatcgatgag cccaacgaat ggaagctgct tatcgatcag tatcatttta acaatgacgc
      601 cgccatcact gacctgatca acaagctcgt caggatttgc agcggcccgg aaaacaccaa
      661 agatatctat gccgacctca ccctccgcga gctgctggtc cgcatcgccc aaaaccagca
      721 tctccaacac caggtcaatg gcagcgccca aaattccaac aacagccgtt tgcacttcgt
      781 ccttcagtac attcatgacc accttaccga acgcatcgac gtggatacgc tcagccgcaa
      841 ggcctatttg agccgcaaca tcttcttcaa atggtttaag gaacaattcg gcattacccc
      901 gctggaatat attacccgcg agcgcatccg cctcgcccgc gagctgcttg cccacgaaaa
      961 atatactatc actcaggtca gtctccaatg cggcttcacc gacgtcaact atttcgtccg
     1021 aaccttcaaa aagtccgaag gcctcacccc cggcgcctac cgcgacatcc tccgcaagct
     1081 gccctgatcc ccgtcgccgt cctcctaccg cagcaccgcc cgccctcgcc ttcctttctg
     1141 caccgccttc ctccccctac cccagcaccg ccctcatcgg gaaatactcg atcctcgtca
     1201 gcgcctgcgc cggatccccc tcgatcatct tcctgatctc ctcttcctct tcgactccca
     1261 ccacccccat cccgaacgtg cccgccggat ccaggacagg cccaaacacc accacataac
     1321 cacgggcctg ccattcccgc caatagttag aatgcgccct cataaccgaa aactcttcgg
     1381 gcgtcatcgt ctgtgcaaaa tccggccggc tcggattcag cctgacaaaa aaatattttt
     1441 tctccatgga agtcggtttg gttcgtctaa agataggttt tttccacagc gccccaaccg
     1501 ccgaaccgcc ggcaccccca gcctcatcct cgcgcccgcc ggccaccggc acccccagcc
     1561 tcatcctcgc acccgccggg ccgcccctca cgcccgccgc atcgccctgc gcccggggca
     1621 cagtttttta tggtgtccat cccatgaccc acaccgaaaa tgccccaacc ccggcccaca
     1681 acttctacag ggaaatgctg gtgcaactgc aagccaccgg ggatcccttc cttgtcggcg
     1741 gcggtttcgc tttttcccac tacaccggta tccggcggga taccaaagac ctcgacatct
     1801 tctgcaaacc cagtcaatac cctttcttcc tgcagctgtt cgaccggctg ggataccgca
     1861 ccgaatccac cgacgtaaga tggttggcca aggtcttcaa agacgaccat ttcatggata
     1921 tcatcttcga ctctcccaat agcatctgcc aggtcgatga ctcctggtat ctgcacggca
     1981 aagacgccga attcgaaggc gtagccgttc gcatggtccc cgcggaagag ctcatctggt
     2041 ccaagaccta tgtccaaaat cgtgaaagat tcgacggcgc cgacatcaac cacttattat
     2101 tggcgcaggg caaacaactc gactgggacc gcctgctttt ccggctcgac agacactggc
     2161 atctgctatt ggccgaactg ctcgtttttc agttcgtcta cccatccgaa taccgggaca
     2221 tcattccccg cgggctcatg gacatgctga tccggcgggt cggtcagcaa tacgaaatac
     2281 ctccttccgt ggaaaaactc tgccgcgggc ctctcatcga ccaaacgcag tatgccaccg
     2341 atatccgcga ctggcagtat aaagtaacaa ccataaaaac cgtctgaaaa aacaccgatg
     2401 agcacgcccg aaggaaagac acgtatcgca gctatagcgg acatccatgt ccgcgagacg
     2461 gacaggaact ccctcgccgg tctcttccgc gaggtttcgc atcaggcaga tatcctcctc
     2521 atcgccggcg accttaccaa taccggtgac gaggcggaag ccgaagtgct gaaagaagag
     2581 ctgaagacct gctccatccc cgtcatcgcc gtgctcggca atcatgatca tgaaaaagga
     2641 cggcagaaga ccatccgcca gatcctgcag aacgatcagg tgcatatcct cgacggagaa
     2701 gcgttcgcaa tcggaaatgt cggtttcgcg gggatcaaag gattcggcgg cggtttcgac
     2761 aattatatgc tgtccatgtt cggcgagtcg gcaatgaagg cctttgtaca ggaggccgtc
     2821 aacgataccc tgagcctcga acgggcgctg gcccgcctcg atcagcaatt cgacggcctc
     2881 aaaaagatcg cccttctcca ttattctccc acacatgata ccgtcaaagg cgagccggaa
     2941 cagatatatc cctttctcgg cagctcccgc ctggccgaac cccttatccg ccgaaacgtc
     3001 acggccgtct tccatggcca tgcccatgcc ggtacgctcg aaggccagct ccccggcggc
     3061 attcgcgtct tcaacgtcgc ccgccccgtc ctgctcaaag caggtctccc gtctccattc
     3121 tttctgctcg aagtctgatc tcgcctcctg cgccaccgcc gcggcgccaa ctcactcccc
     3181 accgacgtct cacaagccag cttcctttcc accgccgcgg cgccaactca ctgccagccg
     3241 aagcctcgcc tccttccttc ccctacgccc cacagcgtac tccccgccgc agcctcccca
     3301 gccagcccaa aaaagcgaaa ggcggccccc ttctcaggaa aaccgcccat ggctgaaagt
     3361 caaacccgtt cgcaaatgat gattaccagg tcagcaacgg aggatacaaa ggtagcaccc
     3421 ttgcccttcc caaagaatac cactttggtg gtatgtggag aaatatctac gttccacgcg
     3481 atccgttcca cctccccgcc caccgttaat ctcttgtcta tcaccgccct acgtccttgg
     3541 cagggttttt tgaatcatta ctcttaaaaa cacccatcat gaaaaattca ataaaaaaga
     3601 tcgtcggtat gatgcttctc gcgggcctgc tgatcacagg cttcaccagc tgttacgaaa
     3661 cgacatatta tcaccattat catcaccata cccatgactg gtacggtcac caccatcagg
     3721 aggtacccgt aggcgtggac ttcaatgtcg atgtcgaagc tcatcatcat tagtcatcac
     3781 tccacgatca tcactcttca accaacatga cccgggattc ccctacccgt ggtacccggc
     3841 ctaccggggc ctcacccggg tcattctttt ctactcacca gttccacttc tttcaccttc
     3901 tccggaaacc ataaggtcat ttctcccttc ccccgattgg cccacgcata atagggtatc
     3961 agctctatcg gggcgtcccg ggtcgataca ttcaatccat cttctccgac tacaacccgc
     4021 acccccgccg cactgatcat attcactccg gccaaaacct tcccggccct caaagaaaac
     4081 ccgctcttca ccggaaggat cagattgctg gtcctccccg aattgtcggc ccactccgca
     4141 caatacacca acggaccaca ctgcaacgcc acttttccga tatcggcttt cacgctatcg
     4201 caagcctcca ccatctgcgg cttcatcggc aacaccagct ctaccgaatc accattcttc
     4261 catttgcgat cgatcaccgc gtaaccctgc tgcatcggcg catccactac ctgcccgttg
     4321 accttgacca tcactctctc tgtggcctca ccagcaaatc gatacaaacc accaggtatc
     4381 gcctcgccac gtgcccaccc cggtatccgt aacaacaacc ggaaaggcat cggccgcccc
     4441 acctctacgc gcaatcgaat atctccattc aaaggataat tgctttgctg ctcaagcccg
     4501 atcgcctgca tgccagcggc cgccttcgcg gtctccaccg cccccaactc aatcttcgcc
     4561 tttccactaa catataagtt cacatacaca tccctgcccc tcaccgcata cacatacccc
     4621 ggcaccgacg gcaaaaatct cgcgagattc gtcgggcagc acgaacacgt aaaccagccc
     4681 gcgcgctccg gttccaggtc aggatacgga tgcccgttca ccacctccat cgcattcgta
     4741 taaaaaaagc tcttcccatc caatcccacc cccgacagca atccattata aagcgtcctc
     4801 tccatcacgt cgatatattt cgcatcccca tgcaggagga acatccggta gtcccagaaa
     4861 acgcttccca ccgccgcaca ggtctctgca taagccgttg cattcggcag ctcatagttg
     4921 tcgccgaacc gctctccgtc cccgatcgcc ccgatccctc catgcagata catcttcttc
     4981 cctaccatat tgtcccaaag gcggtcgacc gcccgaacat aagtactgtc ccccgtcaat
     5041 gcagcgacat cggtcatcgc cgaatagaga tacatggccc tgacagcatg tcccaccact
     5101 tcatcttgcg cggtcaccgg ctcatgcgcc tgccagtacc gtccgttctt ccacacatcc
     5161 gtgcttgttt tatcgtactg cctctccccg cgcatctcca ggaaaaactt cgccagatcc
     5221 agatattcct tccgccccgt caccctataa agcttgacca gtcccatctc tactacttca
     5281 tgacccggag cgacatgttt cttgtcaggt ccgaaagtcc gcaccagcag atccgcattc
     5341 tttaaagcga tatccaaaaa attccgccgg cccgtggcat tgtaatgcgc caccgcggct
     5401 tcgaaaagat gccccgaaca atacagctca tgactcagct cggcctcatg catccaccgc
     5461 tcgggaccag cccaggggtg aacatgctgc ggatcgatcg ttcgggcggt ataaaggtat
     5521 ccatccgcct cctgcgcccg gccgatgaga ccgatcaagg agtcgagata agccgccagt
     5581 gcaggatccg ggtgcaccgc cagcgaatag gacgcccctt cgatggtttt gtagatatcg
     5641 gtatcgtcga agggatatgt cgtgcagaac tttcctgtac gcgcagcggc catctcgaaa
     5701 ttatggaccc gtcccgtcgt ccggcatcgc tcgaaagagg cggggatcgt caccgtcctg
     5761 ttcgtctcga tcctcggcag ccagaacccg tcattcacag tcaccgctgt aaacggtacc
     5821 ggacttatcg gataatctct ctgtcccgcc gccggttgcg ccatcgcgac cccgcaagcc
     5881 gcgaccagta taaaacataa cattgttttc atacgcccaa tttacctttt attcggtaaa
     5941 tttaatcccc accaaaccat cttcttatgg gcgtcctctt ccaaaccttt tactggaact
     6001 gcccgtccct cgaaaatcaa gcctatcaat ggttcaatta tgtcaaaccc cggataacct
     6061 cccttgccgc gacaggcttc accgccattt ggcttccccc cttctgcaaa gctgcctgca
     6121 tcggcggtcc gtccatgggt tatgatccct acgattactg ggatctcggt gagctcgacc
     6181 agaaaggcgg tatccccacc tggttcggtt ccagggccga tcttctcgcg ctcatcgccg
     6241 aagcgcatac tcacaacatc gatgtctatg ccgacctcgt attcaatcat aactcaggcg
     6301 cggacgctga ggagctgaac cctgtcacca acagcatggg ctggacgaaa ttcacgccgg
     6361 ccagcggcaa gttcccccgc gactgggaat gttttcatcc gtcggaatac gagaccaccg
     6421 acggtttttc cttcggcggg cttcccgatc tctgtcatcg caatcccgat gtctatacct
     6481 ctctcctcga atatacccgc tatctgctcg ggaccatcgg catcgacggg ttccggtatg
     6541 actgtgtcaa aggctatgga ggctggatgg tacgtgccat ccaggagctc cgcggtcttc
     6601 gaaatgacat cgcctatcgt cctttcggtg tcggcgaatg ctgggatacg gacaggacca
     6661 tactcgactg gatgaccgaa gccaatgcct
//
LOCUS       DASYSV010000190         3813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1697341, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000190 DASYSV010000000
VERSION     DASYSV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697341"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VGQ51_05795"
     CDS             complement(<1..601)
                     /locus_tag="VGQ51_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV9036109.1"
                     /translation="MQYSNNYNGRISAADAKGTNTDSEESYRCLFEQATDLIVIHDMD
                     GVIVNVNDSFCGSIGFTRDELLKMNYRDLIDPEQLKIQPIRMQELSEGKRIFSERRYV
                     CKDGSLIEIEANVKKMSNNLVMGVARDVTDRKKMERELRETELKFRLISERSLVGIYI
                     FQDGKFAYVNPKFAEIFGYSQEEMINSFDVDAIVHPEDCD"
     gene            1135..1917
                     /locus_tag="VGQ51_05800"
     CDS             1135..1917
                     /locus_tag="VGQ51_05800"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEV9036110.1"
                     /translation="MKTVPTPGKFLLLSLSIIILPILAEAKKKSSVSDVAGRNITYSH
                     RARKTHFSARMIERRMIALEAAQMFSEMQLQDSGLNQRALLYALLGYHRLLKRHLLRK
                     TQVLSICDFSQSSGSRRMYVIDVRNRRLLYRTYVAHGINSGHEYACSFSNRPQSYKSS
                     LGFFVTRNAYFGSNGLSLRIDGVERGINDKARQRNIVIHGAPYVSERILHKYGVMGTT
                     FGCPAIPQEMASQVIPVLKNGTCFFVYYPSRRYLSGSGVING"
     gene            1960..2724
                     /gene="nth"
                     /locus_tag="VGQ51_05805"
     CDS             1960..2724
                     /gene="nth"
                     /locus_tag="VGQ51_05805"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV9036111.1"
                     /translation="MPPKTNWPAAIRPLLEKYNGKRHPLQHRNTYQLIVMVVLSAQST
                     DDVVNQVAPAFFNAYPNLAALSAVRPEDLFPLIGKIRNFGHKAAWLVAIAKQLGTDEH
                     IPLTMDELTALPGIGRKSASVILREAGKAPEGVIVDIHVVRVAPRLGIAKDPDPKKME
                     QQLMKKLPRDEWDAGMAMSFLGREICRPKPECERCLMNPVCAYYARSKKTEAGAAKKT
                     AEGSPKKTGQPKKTRVKSPEKTVKEKVKKRAGRSGK"
     gene            2740..3516
                     /locus_tag="VGQ51_05810"
     CDS             2740..3516
                     /locus_tag="VGQ51_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV9036112.1"
                     /translation="MNHPEPQMAGKQNAVDIAPLLTILNHFHPVSPAIESYLNQHVDV
                     YKVRKRKLLHKEGQPCEHIYFILKGAIRGFTREGQKDITTWINVENQLVSSIFSLTYR
                     APSIENIQALENCELLIMSLDELEALYERFPEANLIARKLLQEYYADAERRAFIARLT
                     KAENRYRHFLIHHRHLVNRIPLKYIASYLGMTLETLSRVRKKFSVGLMLPGPTAVPIA
                     LPIHRDPAAVSIPPPIHRDPTAASSPPPTHRDPTAGKYSS"
     gene            complement(3476..>3813)
                     /locus_tag="VGQ51_05815"
     CDS             complement(3476..>3813)
                     /locus_tag="VGQ51_05815"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEV9036113.1"
                     /translation="LEMIAGSGCTPFLNVFKRMGEGQGILSFPFKGYTLAIDFPVTKS
                     LLSFTPKLDAKVLSAGGRLYLGKDALLHESTFKAMYPQHEKWMAIKRKYDPGNRFSSN
                     ISRRLGLDV"
BASE COUNT          968 a         1032 c          915 g          898 t
ORIGIN      
        1 gatcgcagtc ttcgggatgg acgatcgcat ccacatcgaa cgaattgatc atctcctcct
       61 gcgaatagcc gaagatctcg gcgaatttcg ggttgacata tgcaaatttc ccgtcctgga
      121 aaatatagat gccgacaagc gaccgctccg agatcagcct gaacttcaat tcggtctcgc
      181 gcagctcgcg ctccatcttt ttgcgatcgg taacatcacg ggcgactccc atgacgagat
      241 tgttggacat tttcttcaca ttggcctcga tctcgattaa ggacccgtcc ttgcaaacat
      301 accggcgttc cgaaaagatc ctcttccctt cgctcagctc ctgcatccgg atcggctgga
      361 tcttcaattg ttccggatcg atcagatccc ggtaattcat tttaagcagt tcgtcgcggg
      421 taaagccgat actgccgcaa aagctgtcat tgacattgac aataacgcca tccatgtcat
      481 ggatgacgat cagatccgtg gcttgctcaa aaagacaacg ataactttct tcactatcag
      541 tattcgttcc cttcgcatcc gccgcagaaa tgcggccatt gtaattattc gaatattgca
      601 tggtttacac taaactttct ccagtcactt tcggtaggca atcgtgggca atcctgtcat
      661 aaaggattcg atagggcggt cggaaaacat ggtctatggc ggtctacggt tggaaaaaaa
      721 gggcggatta aatgatgttc atccgggcac gtaagctgaa tttttatact ctttcagagc
      781 tctttttttc aaaagaagcg cgaaggtatg gcaaatcgac caagcggcat gcattattca
      841 aaaaaatatg cgtttttttg cgggtatttt cggttatgtt tggtttttag cttactagaa
      901 ataacacaaa attaatattc gaatgttggt cggtggaaaa actgtgaatt gacttgcatt
      961 tttggctttt ggcgactaca tttgttatcg cagtacccaa tctccctgaa aggcctttcc
     1021 gctaaatccc atctcctttt ttaagctttt tttcaattat cctttgtaat tgccggcgta
     1081 ttcctatgga attccctaaa ggttcctaat ccgtaacacc cgcaaacttc ccttatgaaa
     1141 acagtaccta cgcccggtaa gttcttactc ctttctctat ctattatcat cctgccaata
     1201 ctggcagagg ccaaaaagaa aagttccgtc agcgatgtcg ccggccggaa catcacctat
     1261 tcccacaggg caagaaaaac gcatttctcc gcccgcatga tcgaaagacg gatgatcgcg
     1321 ctcgaagccg cccaaatgtt cagcgagatg cagctgcagg actccggatt gaatcaaagg
     1381 gcgctgcttt acgcgctcct cggctatcac cgcctgctga agagacatct gctccggaaa
     1441 acacaggtat tatccatctg cgatttcagc cagtcttcgg gctcaaggag gatgtatgtc
     1501 atcgacgtgc gcaaccgacg attgctttac cgaacctatg tcgctcacgg catcaattcc
     1561 ggccatgaat atgcctgctc tttctcaaac aggccgcaat cttacaaaag cagcctgggc
     1621 ttcttcgtga cccggaatgc ctattttggc agcaatggcc tctcgcttcg gatcgatggg
     1681 gtcgaacggg ggatcaatga caaggccagg caaaggaata tcgtgatcca tggcgcacct
     1741 tacgtaagcg aacgtatcct gcacaaatac ggggtgatgg gcacgacctt cggctgtccg
     1801 gccattcccc aggaaatggc ctcgcaagtc atccccgtgc tgaagaacgg gacctgcttt
     1861 ttcgtttact acccctccag aagatattta tcggggtcgg gggtcatcaa tggatagttt
     1921 tcgcgggcag tcagtacctt cattcgcaaa tcagaccaaa tgcctccaaa aaccaactgg
     1981 ccggctgcca tccggccgct gctggaaaag tataacggca aaaggcatcc gttgcaacac
     2041 cggaatacct atcagctgat cgtgatggtc gtcctctccg cccagtccac cgatgatgtc
     2101 gtaaaccagg ttgccccggc attcttcaat gcctatccga acctggcggc tttgtcggcc
     2161 gtcagacccg aggacctttt cccgctgatc ggcaagatcc ggaatttcgg tcataaggcg
     2221 gcgtggcttg tcgccatcgc caaacagctc gggacggatg agcatattcc tcttaccatg
     2281 gatgagctga ccgccctacc cgggatcggc agaaaatcgg ccagtgtcat cctgcgggaa
     2341 gccggcaagg ccccggaagg ggtgatcgtg gacatccatg tcgtccgtgt ggcgcccagg
     2401 ctgggtatcg cgaaggaccc ggatccgaaa aagatggagc aacagttgat gaagaagctt
     2461 ccgcgcgacg agtgggacgc cggcatggcg atgtcctttt tgggacgcga gatctgccgc
     2521 cccaaacccg aatgtgaacg gtgccttatg aatcccgtct gcgcatatta tgcccgtagc
     2581 aaaaagacag aggcaggggc agccaaaaag acggcggaag gatcacctaa aaagacaggt
     2641 cagccgaaaa aaacacgggt gaaatcaccc gaaaagacgg ttaaggaaaa ggtgaaaaaa
     2701 cgggccgggc gcagcgggaa atagatttag atttagtcca tgaaccatcc cgaacctcag
     2761 atggcaggca agcaaaacgc cgtcgatatc gctccattgc tcacgatcct caatcatttt
     2821 cacccggtga gccccgccat cgaatcctat ttgaatcaac atgtcgatgt atacaaagtg
     2881 cgaaaaagaa agctcctgca taaagaaggg cagccttgcg agcatattta tttcatcctg
     2941 aaaggggcga tacgggggtt cacccgggag ggacaaaagg atatcacgac ctggatcaat
     3001 gtggagaacc aactggtatc ctccattttc tctctgacct atagggctcc ctcgatcgaa
     3061 aatatccagg ctctcgagaa ttgcgagttg ctgatcatga gtcttgacga gctggaggcg
     3121 ctgtatgaac ggttccccga agccaacctc atagccagga aattattaca ggagtattat
     3181 gcggatgccg aaagaagagc tttcatcgca cgtctgacga aggcagagaa tcgataccgg
     3241 catttcctta tacatcaccg gcacctggtc aaccgcatcc cgttgaaata catcgcctcc
     3301 tatctgggca tgacgctgga gaccctcagc cgtgtaagaa agaaattttc agtggggctt
     3361 atgcttccgg gcccaaccgc tgttccgatc gcgctaccta tacatcggga cccagccgcc
     3421 gtttcaatcc cgccacctat acatcgggac ccaaccgctg cctcatcgcc cccacctaca
     3481 catcgagacc caaccgccgg gaaatattcg agctgaacct gttgcccgga tcatatttgc
     3541 gtttgatggc catccacttt tcatgctgcg gatacatggc cttgaaagtg ctctcgtgca
     3601 gcaacgcgtc ctttcccagg taaagcctgc cacctgccga caatactttg gcatcgagct
     3661 tcggtgtaaa cgacagcaga ctcttcgtca ccggaaaatc gatcgccagc gtataaccct
     3721 tgaaggggaa agagaggatg ccctgcccct cacccatgcg tttgaataca tttaaaaaag
     3781 gcgtgcatcc gctgccggcg atcatctcca ata
//
LOCUS       DASYSV010000191        12131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_36236, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000191 DASYSV010000000
VERSION     DASYSV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36236"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2233)
                     /locus_tag="VGQ51_05820"
     CDS             complement(<1..2233)
                     /locus_tag="VGQ51_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9036114.1"
                     /translation="MTIQRLLAKMASFTLLCVLFTQAAFSQTKTVSGTVTDDKGAPIQ
                     GATITVKGTKAGTSTAVDGKFTLSVPSNATTLVVTSVGFSSQEVAIANQASFSVTLTA
                     SQANLNEVVVIGYGTAKKKDFAGSLTTVGAKEFNKGVVTSPDQLMQNKVSGLEVVNNS
                     GQPGTATTVKIRGNSSIRGTGNPLYVVDGVLLDGRNARPGVNLGTGGFGQTPDANPLL
                     YINPYDVSDITILKDAAATAIFGSRGANGVVMITTKKPTGGGVKLEANISEGWNVGYM
                     KKFDVLSASDYRSALKKYNLDTITVNPLDHHQNVDALDEVTQHSTIQNYAVAMSGGSD
                     LGHFRASFLASKTPGLVKSNDLDKYIGTFAGSYKFFDQHLTIDFSLIAGHTNEDIVLA
                     SNTAGSAGNLFSSALQWNPTLSMTDANGHYVFPSNGSGNPLAFIDGYKDRAKVNTFLG
                     YISADYKIVKNLDYKFLYSINESAGSRGTNIDGWITGIPPISGAGFGIVSNAEMISQV
                     TDNTLNYHVDLTPNVNLTATAGYEYWKSDYNTNQFSALGFNFNLDQANRLDIKYSDVM
                     QDGNAQQVPFSEKDITTEVQSFFGRVGVNFSDKYYLTGTIRADGSNKFGSANKYGYFP
                     SFSGKWVVSNEDFLKTSSLFSNLALRATWGITGSQDFPSGSAIDQFNFITYNTGQQTN
                     GGNTHLKWEETKQIDIGLDFSLLNNRLWGTFDYYDKNTTNILFETTPIQPAPPANQWQ
                     NLPG"
     gene            complement(2405..3073)
                     /locus_tag="VGQ51_05825"
     CDS             complement(2405..3073)
                     /locus_tag="VGQ51_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036115.1"
                     /translation="MYETFLTFNHTSFRYNYLCHYLPIAAGMNTLSRSLMKFKRGAHP
                     DLEAWIDSAREALAEKPPSGDSILLRALGHRELTAHNTSSPNPHSELTPQNIRPSSLD
                     LLCRTLALDFGCLYLPTLLSKTRETLSNKNLSRPEREAQLLDVYSADIIDAPASYLII
                     DDILTTGATIHAIIAALRRKSSTCPIRVFTLARASNNPLPGPIIPLHGRSFRFDPGSG
                     WVQS"
     gene            3093..3761
                     /locus_tag="VGQ51_05830"
     CDS             3093..3761
                     /locus_tag="VGQ51_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV9036116.1"
                     /translation="MENETQNLLYWEELFVDYWKAQEGIADGSHDLGHFQRVWKAARH
                     IAREEGGSADLLVLLASAYFHDVVSMPKDSPRRSESSRLSAERAGELLVSLGFPAGKI
                     EGVRHAIHAHSFSAGVPALTEEAKILQDADRLEAVGAIGVARCFYTAGQMDARLFDAA
                     DPLAKGREPNDRVYALDHFQLKLFKLPGMMKTATGRRLAEENAEWMRGFVSRLCGEIE
                     GDFG"
     gene            3919..4692
                     /locus_tag="VGQ51_05835"
     CDS             3919..4692
                     /locus_tag="VGQ51_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV9036117.1"
                     /translation="MEEQESLDQFLDRAGRDFLAHISLDCVVFGFHAGQLKILLLKMK
                     HEDLLALPGGFVMKDETMEQAAIRVLRERTGVENVFLQQYHVFSDVLRSDPARRPLIV
                     ERLGIPPEKAQFLAQRFISVGFYALVEFSRVVPRPDLLSETCDWYSLEDCGPLILDHG
                     RILETALTELRTQLNYQPVGYNLLPRKFTLPELQKLYEAILGRSLDRRNFQRKIFSYG
                     IVRALDEKRTGVAHKAPNLYSWDLRKYQQALKAGLGGGW"
     gene            4874..7333
                     /locus_tag="VGQ51_05840"
     CDS             4874..7333
                     /locus_tag="VGQ51_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEV9036118.1"
                     /translation="MKKLLPVLCLLLLIINAAYAQTQVCPLNSNFSLGNLTHWQAYTG
                     NNASGNPTRDSIHYDSLTVAPGGTLGATQIQEYNLPSVTGIQIISASSIDPFGGFATI
                     PRINGYQYTNTIKLGSTTITHSSAGGTGGGYVRGVSMRISVPPGPVTTPYTMTYAYAM
                     VLENGAHNTNQQPLFTATLRTADSVIQCASPAYELPTFNNASQGGGNATLDSAAARAN
                     GFYPSTQPSPNPNPNGVGVNSGQHLFDVWAKNWTEVTFDLAPYRGQQVTLTFETDNCV
                     PGGHFAYSYIALRSTCGGLLISGDTVACIGSTLTYSVPGLGGATYQWQVPGDWTVVSG
                     ADSSILKVLIGNNTGAVTAHEQNGCANLQAVLPVTTSPPTIPGALSGNTEVCSGSNNS
                     VITLGGYRGGVLDWLASTDGGLTYHTVTDTTDQYTAQDLTATTIYRALVQNGESCQID
                     TASPVTVVVDPQTVGGALSPPEMQFCIAQTKDALLTLTGETGVLVNWQTSPDGFTWNN
                     FNPADIDTSYEVSGIVSNTQFRVIVQSGVCPALASVPAQIDIVKTAFPQATTEPSDTL
                     ICYGTTATLNAQIITATSFNWTSGVSTLTNQGNGTIDLLPYNIQATARPLKTTNYVLS
                     MVNAGCPNSLKDTFLVRVLPPITVDAGDDTAVVVNQPLQLQASSNDTTGDDTFNWTPA
                     TGLNNPNIADPVGLYTEADSIRYLITATSGNGCKGTASILVKVYGTGPDIFVPNAFTP
                     GGATNNIFRPIPVGIATLRYFRVYNRWGQMVFSTTRIGDGWDGRLNGQSAEGGTYVWM
                     LAGTTYMGRLVVHKGTMVLVR"
     gene            7446..9824
                     /locus_tag="VGQ51_05845"
     CDS             7446..9824
                     /locus_tag="VGQ51_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV9036119.1"
                     /translation="MRKIYFLGVWVVLLSMTEAVTAGPIRDTASLRREIMAVYARQPQ
                     ADFAIAFEDLSTGQQFFFNEHESFHAASTMKTPVLIETFRLIAAGRLSLEDPVLIHTD
                     FISIADSSSYRLDSADDSEKDLYGLAGRRLPLKQLLYKMITESSNLATNLVIELVGAK
                     NVMATMREMGAMDIRVLRGVEDNKAFAQGMNNTTSAYDLMLLLRSIASGTAVDPGASR
                     AMIRILLDQHFKEVIAGNLPDNVRVASKSGWITGSVHDSGIVFLPDGRKYVIVLLSRG
                     IADHGLAVEIGAAVSKIFYDHMVEGPPPGRAGIDDIKGYFPVIEGMYKAYAEKNHFPG
                     MAFGIVAGGKLVYSGGTGYSNPGAEDGGRGVRVPATAETDFRIASMTKSFISVSILQL
                     RDAGKLRLDDPASLYIPELRGQKGPFSDAPVITIRHLLTHSAGFPEDNPWGDRQLADT
                     DEQLLAMVRKGLSFSNSPGIAYEYSNTGFALLGYILQKVSGQKYSDYVTEHILKPLGM
                     THTYFEYSRVPSGSLAHGYRWLGGEWVEQPMLHDGAYGAMGGMITTMEDFAKYEAWQL
                     AAWPSRSGPEEGVLKRSSRREMQQPWVFNNLNALFRYSPGGEACPMTSAYAYGIRWTK
                     DCKGRTMVGHTGGLPGFGSNWMILPDYGIGVICFANLTYANTSAINMRVLDTLVTLAR
                     LKPREGVASSVLLQRQKELVALLPDWNGALQSPAFAVNFWMDYFVDTLRAESRALFAK
                     VGTIRGVGPMHAENGLRGWFLLEGEKGDLEVRFTLTPENPAKVQEFHLRAVD"
     gene            9835..10710
                     /locus_tag="VGQ51_05850"
     CDS             9835..10710
                     /locus_tag="VGQ51_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEV9036120.1"
                     /translation="MDRTFKKTRLAPTPSGYLHVGNVLSFSLTAALARRTGASVLLRI
                     DDLDIERVRTEYVRDIFGTLRFLGIPWDEGPADVAAFERDHSQVQRMELYSRALGSLR
                     RSGLLFACTCSRSDLRDGIYPGTCRDRGLSLDDPGVSWRLRTSASLSLHVKTWDRGMV
                     GEVLPIEMTDFVVRKKDGFPAYQLTSLVDDLHFGVDLIVRGADLWPSTLAQQHLALCV
                     PGAEAFREATFYHHPLLVGGGGEKLSKSAGATSIRYMRGEGLAVGDIYTSIARLLGRE
                     EVVRGWEELAGLILG"
     gene            complement(10742..11185)
                     /locus_tag="VGQ51_05855"
     CDS             complement(10742..11185)
                     /locus_tag="VGQ51_05855"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV9036121.1"
                     /translation="MLRQIAQLYLRLAAGIGFFVLGLDRLGAWGPYGKPWVSWGDWKH
                     FSIRAHELMSFLPYNVAETFAMIATFVEVTGGFLLVIGLFTKQAALACSALTLCFSIA
                     MAITDGITSPMNYSVFTVSAACLLLASLGEYRYSLDGLIAKKRLP"
     gene            11772..>12131
                     /locus_tag="VGQ51_05860"
     CDS             11772..>12131
                     /locus_tag="VGQ51_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036122.1"
                     /translation="MQKIPALCGAALLCLSLNISYAQVVDSVSGRLLNYPSRLFARLQ
                     SKLSGLNNDLGTQTQKYLDRMSRREQQLQRKLISVDSVGAQRLFAGSAAQYAALIQRL
                     KSDTGKAHQALSGQYRPY"
BASE COUNT         2542 a         2683 c         4022 g         2884 t
ORIGIN      
        1 tgcccgggag gttctgccat tggttggccg gaggagcggg ttggatcggg gtggtctcga
       61 agaggatgtt ggtggtattc ttgtcgtagt aatcgaaggt gccccagagg cggttgttca
      121 gcagggagaa gtcgagaccg atgtcgatct gcttggtttc ttcccatttc agatgagtgt
      181 taccgccgtt ggtttgttgc ccggtattgt aggtaatgaa attaaactgg tcgatagccg
      241 aacccgaggg gaagtcctgg ctacccgtga tcccccaggt ggcacggagc gccaaattgg
      301 aaaacaggct gctggtcttc aggaagtctt cgtttgagac cacccattta ccgctgaagg
      361 aggggaaata accatatttg ttggcggagc cgaatttatt ggagccatcc gcgcggatcg
      421 taccggtcag ataatacttg tcggagaaat tgacgcctac gcgtccgaag aaggactgga
      481 cctcggtggt gatatctttt tcactgaagg gaacctgctg tgcattaccg tcttgcatga
      541 catcgctgta cttgatgtcc agccggtttg cctggtcgag gttgaagtta aagcccagcg
      601 cgctgaactg attcgtgttg tagtccgatt tccagtattc gtaaccggct gtggcggtga
      661 ggttaacatt cggggtcagg tcgacatggt agttaagcgt attgtcggtg acctgcgaga
      721 tcatctcggc gttcgaaacg atgccgaagc ccgcgccgct gatcggggga atacccgtta
      781 tccagccatc gatattggta ccgcggctac cggcgctttc gttgatcgag tacaggaatt
      841 tgtaatccag atttttgacg atcttataat ctgccgagat atagccgagg aaggtattga
      901 cctttgcgcg gtctttatat ccgtctatga aggcgagggg attgcctgaa ccattgctgg
      961 ggaaaacata atggccgttg gcatcagtca tggacaacgt ggggttccac tgcagggccg
     1021 agctgaacag gttgcctgcg gacccggcgg tgttggaagc gagcacgata tcttcgttgg
     1081 tatgaccggc gatcaagcta aagtcgatgg tcaggtgctg atcgaagaat ttgtagctgc
     1141 cggcgaaggt accgatatac ttgtccagat cgttagactt taccaggccc ggcgttttcg
     1201 aagcgaggaa ggaggcgcgg aaatgaccga gatcgctgcc gccgctcatc gccactgcgt
     1261 agttctggat ggtgctatgc tgtgtgacct cgtcgagtgc gtccacattc tggtggtggt
     1321 cgagcgggtt gaccgtaatc gtatcgagat tgtatttctt cagtgcagaa cggtaatcgc
     1381 ttgcgctaag gacatcgaac tttttcatat aacctacatt ccagccttcg gagatgttgg
     1441 cctccagttt aacaccgccg ccggtgggtt ttttcgtagt gatcatcacg accccgttgg
     1501 cgccgcggga accgaaaatg gcggtagctg cggcatcttt gaggatggtg atatcggaga
     1561 catcataggg gttgatgtac agcagcgggt ttgcgtctgg ggtctgaccg aatccgccgg
     1621 tacccaggtt aactcccggg cgggcgttcc taccgtcgag gagaacaccg tctaccacgt
     1681 acaacgggtt acccgtgccg cggatggagg agttacccct gatctttact gtggtggcgg
     1741 tgccgggctg gccgctattg ttgaccactt ccaggcccga gaccttgttt tgcatcagct
     1801 ggtcggggga ggtgacgaca cctttgttga attccttggc gccgactgtg gtcagcgaac
     1861 ctgcaaagtc ttttttcttg gccgtgccat aaccgatgac tacaacttca ttgaggtttg
     1921 cctgagaggc tgtcagcgta acggaaaaac tggcctggtt ggcgatggcc acttcctggc
     1981 tggagaaacc gacggaagtg acgaccaggg ttgtagcgtt ggaaggtacg ctgagggtga
     2041 atttaccgtc gacggcggtg gaggtcccgg ccttggtgcc ttttactgta atggttgcgc
     2101 cctgaatggg tgctcctttg tcatctgtaa ctgttcctga gactgtcttg gtttgggaga
     2161 atgctgcttg tgtgaaaagt acacaaagca gggtgaagct tgccatttta gcaagcaatc
     2221 gttgaatggt cataaagcaa aagtttagca attaatgatc taaggtaaaa aaattaaaat
     2281 ttacttaaca aatttttttt cccaagagtc accctcgtaa attattgggt gatattggta
     2341 ttggacagtg tgagcgtgtt ttccgctgcg ccttctttcc tttattttag ctaaaccgaa
     2401 ttagctagga ttggacccat cccgaaccgg ggtcgaaccg gaagctgcgg ccatggaggg
     2461 gtatgatcgg gccggggagg gggttattgg aggcccgggc gagggtgaag acgcggatgg
     2521 ggcaggtgga ggatttgcgg cggagggcgg cgatgatggc atggatggtg gcgccggtcg
     2581 tgaggatgtc gtcgataata agataggagg cgggcgcatc gatgatatcg gcggagtaga
     2641 cgtcgaggag ctgggcttca cgttcggggc gggagaggtt cttgttgctg agggtctcgc
     2701 gggttttgga gaggagggtg ggaagatata ggcaaccgaa atcgagggcc agcgtgcggc
     2761 agaggaggtc gagagaggag gggcggatgt tctgcggggt gagttcggag tgagggttgg
     2821 gggaggaggt gttatgtgcg gtgagttcgc ggtggccgag ggcccggagg aggatggaat
     2881 cgccggacgg aggtttttcg gcgagggctt cgcgggcgga gtcgatccag gcttcgagat
     2941 cgggatgggc cccacgtttg aacttcatca ggctgcgtga aagggtgttc atccccgctg
     3001 caatggggag atagtggcag agatagttat atctgaaaga ggtatggttg aaagtgagga
     3061 aggtttcgta catatttaaa attacaatgc gaatggagaa cgagacgcaa aatctattgt
     3121 attgggagga actgtttgtt gattattgga aagcgcagga ggggattgca gacgggtcac
     3181 atgacctggg gcattttcag cgggtgtgga aggcggcgag acatatcgcg cgggaggagg
     3241 ggggaagtgc ggacctgttg gtgctgctgg cttcggctta tttccatgac gtggtatcga
     3301 tgcctaagga ctctccgcgg cggtcggagt cgtcgcgtct gagcgcggag cgggcggggg
     3361 agctgttggt ttcgctgggc tttcctgccg gaaagatcga gggggtgcgg catgcgatcc
     3421 atgcgcatag tttttctgcc ggggtgccgg cattgacgga ggaggcgaag atattgcagg
     3481 atgccgacag gctggaggcc gtgggggcta taggcgtggc gcgttgtttt tatacggccg
     3541 ggcagatgga tgcgcggttg ttcgatgctg ccgatccgct ggcgaaaggg cgtgagccta
     3601 acgaccgggt gtatgcgttg gaccatttcc agctgaagct ttttaagctg ccggggatga
     3661 tgaagacggc gaccgggagg cggctggcgg aggagaatgc cgaatggatg cgggggtttg
     3721 tttcgaggct atgcggtgag atcgagggag atttcgggtg agggagggcg ttggcggggt
     3781 gaggacgttg gtgggggagg acgttggcgg tgtgagggag gacgttggag aggtgaggga
     3841 gggcgttggc ggcgtgaggg agggcgttgg cggaaggttg atgcggcaat gataggtatt
     3901 ttttttattt tgggaagaat ggaggagcaa gaaagcttag atcaattcct ggatagggcc
     3961 ggacgggatt ttctggccca tatttcgttg gactgtgtgg tattcggttt tcacgcgggg
     4021 cagctgaaga tattattatt gaagatgaag catgaggacc tgctggcatt gccgggcggg
     4081 ttcgtgatga aggacgagac gatggagcag gccgcgatca gggtattgcg ggagcgaacg
     4141 ggagtggaga atgtgtttct gcagcagtac catgtattca gtgacgtatt gcgcagtgat
     4201 cctgccaggc ggccgctgat cgtcgaacgg ttggggatac cgcctgagaa agcgcagttc
     4261 ctcgcccagc ggttcatcag tgtgggtttt tatgcactcg ttgaattttc gagggtagtt
     4321 ccgcgtccgg accttttgtc ggaaacctgt gattggtata gcctggagga ttgcgggccg
     4381 ttgatcctgg atcacgggcg catattggag accgcgttga cggaattgcg gacacagctg
     4441 aactatcagc ccgtgggata taatcttcta ccgaggaagt tcacgctgcc cgagcttcaa
     4501 aaactatatg aggccatatt ggggaggtcg ctggaccgga gaaattttca gcggaagata
     4561 ttttcttatg gcatcgtgcg ggcgttggat gagaagcgga ccggggtggc tcataaggcc
     4621 ccgaatttgt atagctggga cctgcgtaaa tatcaacagg cgctgaaggc cgggctaggg
     4681 ggaggatggt aaaggatatc tatttacttg ttgatattca atatattatt tagtcggttg
     4741 tgaatgagga aaaaaataaa acggaggccg gtaaactctt gaaggaattc tttcgtaatc
     4801 gtatgggaag gggtaacggc ggagcctggt tccgctggac gaggaggccg gaaatccgta
     4861 ttttgcagta accatgaaga aacttttacc tgtattatgt ctgcttttac tgatcattaa
     4921 tgccgcctat gcgcagaccc aggtatgtcc tctcaacagt aatttttcgc tgggaaatct
     4981 gacccactgg caggcgtata ccgggaataa tgcctcgggg aaccctacca gggactcgat
     5041 ccactacgat tctttgacag tggcacccgg gggtactctc ggcgccacgc agatacagga
     5101 atacaacctg ccttcggtga cgggtatcca gatcatatcg gcgtcttcga tcgacccgtt
     5161 cggcgggttt gccacgatcc ccaggatcaa cggctatcag tatacgaata ccatcaagtt
     5221 ggggagcact accattaccc attcgtcggc cggcggcacc gggggaggat atgtgcgtgg
     5281 ggtgagtatg aggatcagcg ttccgccggg tccggtgacg acgccttata cgatgaccta
     5341 tgcctatgcc atggtactgg agaatggggc gcataacacc aatcagcagc ctttatttac
     5401 cgctacgctg cgtacggccg acagcgtgat ccaatgtgct tcgccagcct atgaattgcc
     5461 caccttcaac aacgccagcc agggcggcgg caatgcgacg ctggactcgg cggccgcgag
     5521 agcgaacggg ttttatccca gcacccagcc atctcccaac ccgaacccca atggggtcgg
     5581 ggttaactcc ggtcaacatc tttttgacgt ttgggcgaag aactggacgg aggtgacctt
     5641 cgacctggcc ccgtatcgcg ggcagcaggt gaccctgacc ttcgagacgg acaattgtgt
     5701 tccgggagga cattttgcct attcctatat cgccttgcgc agcacgtgcg gcgggttgtt
     5761 gatcagcggg gacacggtag cctgtatcgg aagtacgctg acctattcgg tccccggtct
     5821 tggcggggcg acctatcagt ggcaggtacc gggcgactgg acggtggtat ccggcgccga
     5881 cagcagtatc ctgaaagtgt tgatcggtaa caatacgggg gcggtgacgg cgcatgagca
     5941 gaacggctgc gccaaccttc aggcagtcct tccggtaacc acctccccgc ccactattcc
     6001 cggcgcgttg agcggcaata cggaggtttg ttcgggcagt aacaactctg tcatcacgct
     6061 cggcggctac cggggcggcg ttctcgactg gctggcttcc acggacggcg gcctgaccta
     6121 ccatacggtg acggatacga cggatcaata taccgctcag gatctaacgg cgacgactat
     6181 ctaccgggcg ctggtgcaga acggggagtc gtgccagatc gatacggcct cgcctgtgac
     6241 ggttgtcgtg gacccgcaga cggttggcgg cgcgctgagt cctccggaga tgcagttctg
     6301 tatcgcccag acgaaggatg ccttgctgac gctgacgggt gagacgggtg tcctcgtgaa
     6361 ctggcagacg tcccccgacg gatttacctg gaacaatttc aacccggcgg atatcgatac
     6421 ttcctacgaa gtgtcgggca tcgtaagcaa tacgcagttc cgggtgatcg tgcagagcgg
     6481 ggtttgtcct gccttggcca gtgtcccggc gcagatcgac atcgtaaaaa cggcgttccc
     6541 gcaggcgacg accgagccat cggatacgct tatctgttat ggcaccacgg cgacgctgaa
     6601 tgcgcagatc atcaccgcca cgagcttcaa ctggacctcc ggggtgtcga cgctgaccaa
     6661 ccaggggaac ggtactatcg atcttctacc ttataatatt caggctacgg ccaggccgct
     6721 gaagacgacc aattacgtgt tgagcatggt caatgccggg tgtcccaact cgttgaagga
     6781 tactttcctg gtgcgggtgc tgccgcccat cacagttgat gccggagatg atacggcggt
     6841 ggtggtcaat cagcccctgc aattgcaggc aagctccaat gatactaccg gcgacgacac
     6901 cttcaactgg acgccggcca ccggattgaa caatccgaat attgccgatc ccgtgggtct
     6961 gtatacggag gcggactcga tccggtacct gataacggcg acgtcgggca atggatgtaa
     7021 ggggacagca agcatattag taaaagtgta tggaacgggg ccggacatat tcgtgccgaa
     7081 tgcatttacg ccggggggag cgaccaataa tatcttcagg ccgataccgg taggtatagc
     7141 cacactcagg tatttcagag tttataacag gtggggtcag atggtattca gcacgacaag
     7201 gatcggagat ggatgggatg ggcgtttgaa cgggcaatcg gcggaaggtg gaacgtatgt
     7261 gtggatgttg gcgggtacga cgtatatggg gaggctggtg gtgcataagg ggacgatggt
     7321 gctggtgagg tagggggcga ttgcggccgg gggcggcgtg gtcgttgagg gtttgcgccg
     7381 gggttggcgt ggtcgttgct cgttcggccg ggggcgtgag tctgaaaatt tcctatcttc
     7441 cggggatgag aaaaatctac tttttaggcg tgtgggtcgt cctgttatcg atgacggagg
     7501 cggtgaccgc gggtcctatc cgggatacgg cttcgctacg acgggagatc atggcggtgt
     7561 atgcgcggca accgcaggcg gatttcgcca ttgcattcga agacctgagt acagggcagc
     7621 aattcttctt caatgaacat gagagctttc acgcggccag caccatgaaa acgccggtgc
     7681 tgatcgagac cttccggctt atcgcggcgg gccggttatc gctggaggat cccgttctca
     7741 ttcacacaga ttttatcagc attgccgaca gcagcagtta ccggctggac tccgccgatg
     7801 acagcgagaa ggacctgtat gggctggcgg gccgtcggct tcctttgaag cagctgttat
     7861 acaagatgat caccgagagc agtaacctgg ctaccaatct cgtcatcgaa ttggtggggg
     7921 cgaagaatgt catggctacg atgcgggaga tgggcgccat ggatatacgg gtattgaggg
     7981 gcgtagaaga caataaggca ttcgcgcagg gtatgaacaa taccacgagc gcgtatgatc
     8041 tcatgctgtt gttgaggagt attgcgtcgg gaacggcagt ggatccgggg gcttcgcggg
     8101 ccatgatccg gatcctgctc gatcagcatt tcaaagaggt gattgcgggg aatcttccgg
     8161 ataacgtgcg ggtggcgagc aagagcgggt ggatcacggg aagcgttcat gacagcggta
     8221 tcgtgttcct gccggatgga aggaaatatg tgatcgtgct gctttcgcgc ggcatcgccg
     8281 atcatggtct ggctgtggaa atcggggcgg ccgtatcgaa gatcttctat gatcatatgg
     8341 tagaaggacc gccgccgggt cgggcgggta tcgatgatat caagggttat ttcccggtga
     8401 tcgagggtat gtataaagct tatgcggaga agaaccattt tcccgggatg gcgttcggga
     8461 tcgtagcagg tggaaagctg gtgtatagcg ggggaacggg ttatagtaat cccggggcgg
     8521 aggatggagg gcggggtgtg cgggttccgg cgacggcgga aacggatttt cggatcgcat
     8581 cgatgaccaa gagttttatt tctgtgagca ttttgcagtt gcgcgatgcg gggaagttga
     8641 ggctggatga tccggcgtct ttgtatatcc cggagctacg cggtcagaag gggccttttt
     8701 cggatgcacc ggtgatcact atacgccatt tgctgacgca ttcggcagga ttcccggagg
     8761 acaatccctg gggagaccgg caactggccg atacggatga gcaactactg gcgatggtga
     8821 ggaagggtct ttccttttcg aatagcccgg gcattgcata tgaatacagc aatacgggtt
     8881 ttgcattgtt gggttatatt cttcaaaaag tgagcgggca gaagtattcg gattatgtga
     8941 cagagcatat cctgaagccg ttggggatga cgcataccta tttcgagtat tcccgcgtgc
     9001 cttcgggttc gttggcgcat ggatatcgtt ggctgggcgg ggaatgggtg gagcagccga
     9061 tgttgcatga cggggcttac ggggcgatgg gcgggatgat cactaccatg gaggattttg
     9121 cgaagtatga ggcctggcag ctggcggcat ggccgtcccg gagcggtccg gaggaaggtg
     9181 tgttgaagcg gagttcccgg agggagatgc agcaaccgtg ggtattcaat aacctgaatg
     9241 ccttgttccg ttattctccc ggcggtgaag cctgtccgat gacatcggct tatgcttatg
     9301 ggatacggtg gacgaaggat tgcaaaggaa ggacgatggt tggacatacg ggagggttgc
     9361 cgggtttcgg gagcaactgg atgatcttgc cggactatgg cattggggtg atctgttttg
     9421 caaacctgac gtatgcgaat acgtctgcga tcaatatgcg ggtattggat acgctggtga
     9481 cgctggcgag actgaagccc cgtgaagggg tggcctcgtc agtcctgctg cagcgacaga
     9541 aggagcttgt agcgttgttg ccggattgga acggggcttt gcagagtccg gcgttcgccg
     9601 tgaatttctg gatggattat tttgtagata cattacgggc ggagtcgcgc gcattgtttg
     9661 cgaaggtggg gaccattcgg ggcgtcgggc cgatgcatgc agagaatggg ttgcgggggt
     9721 ggtttttgct ggaaggtgag aagggtgatc tggaggtgag gtttacgctg acgccggaga
     9781 atccggcgaa ggtccaggag tttcatttgc gggcggtgga ttaattttgc agtgatggac
     9841 cggactttca aaaagacacg tcttgcgccg acgcccagcg gttacctgca tgtgggcaat
     9901 gtcctttctt tttctttgac ggcggcgctt gcgcggagga cgggagcgtc ggtgctgtta
     9961 cggatcgatg atctggatat cgagcgggtg aggactgaat atgtccggga catcttcggg
    10021 accttgcgat ttttggggat accgtgggat gagggtccgg ctgatgtcgc ggcattcgag
    10081 cgggatcatt cgcaggtgca aaggatggaa ttgtacagcc gggcgttggg atctttgcgc
    10141 aggagcgggt tgttgttcgc gtgtacgtgc agccgttcgg atctacggga tgggatttat
    10201 ccggggactt gcagggatcg gggattatcg ttggatgatc cgggcgtgag ctggcggttg
    10261 aggacttcgg catctttatc gttgcatgtg aagacgtggg atcgtggtat ggtcggggag
    10321 gtattgccta tcgaaatgac ggactttgtg gtgaggaaaa aggatggttt tccggcatac
    10381 cagctgacat cgctggtgga tgatctgcat tttggcgtgg acctgatcgt gcggggtgcg
    10441 gatctttggc cttcgacgct ggcgcaacaa catctggctt tatgcgtgcc gggtgcggag
    10501 gcgtttcgcg aggctacttt ttatcatcat cctttattgg ttggcggcgg cggggagaag
    10561 ttgtctaaat ccgcgggtgc gacttctatt cggtatatgc gaggggaggg tttagctgtg
    10621 ggggatattt atacttcgat cgcgaggtta ttggggaggg aggaagtggt gagggggtgg
    10681 gaggaattgg cagggttgat tctcgggtga ggggcgacgc gtcatgggtg ttttctactt
    10741 tttaaggtag tcgtttttta gcgatcaggc cgtcgaggct gtaacggtat tcgccgaggc
    10801 ttgccaggag cagacaggct gcgctaacgg taaagacgga atagttcatg ggtgaagtga
    10861 tgccgtcggt gatggccatg gcgatagaga agcaaagggt cagggcgctg caggccaggg
    10921 ccgcttgttt cgtgaacaga ccgattacca ggaggaagcc gccggtcact tcgacgaagg
    10981 ttgcgatcat ggcgaaggtt tccgcgacat tatagggcag aaagctcatc agctcgtgag
    11041 cgcggataga aaagtgtttc cagtcacccc agctgaccca gggtttaccg tagggacccc
    11101 aggcgccgag gcggtcgagg ccgagtacga agaaaccgat gcctgcggcg agacggagat
    11161 agagttgtgc gatttggcgg agcatgttgg tgggtttatg ggacaaatgt cgggttttgt
    11221 attgggggat cgtggggcca gatgggagaa aagggagggg ccagggggga tgattggaaa
    11281 gacgaatact tcagtggctt ttctgtagac ggcgcctagg tggtttcact atattttcta
    11341 taatatctta actacaaaac gtcctattcg gtcatttcct ttcccgacac agttgtacac
    11401 gaaatcgaca cgtctatcaa cgatttcgac acgcggaata gatcttaggc gcggtaattc
    11461 gatgagaaaa tactgcagcg taaacgacat gcgtatgcgt tttgactgtg cctaattcgc
    11521 agcgctcttg gaaattctaa aagttttcgt ttacttcgtt ctaatagaat tcctgccctt
    11581 ctctttttct ctaagcctaa ttccacgata ccttaaaacc gcttgctaca tgagagaata
    11641 caaccccctc gcgcgggagc agctacgcgc cccgcctttt tccgacacga ccagtgttca
    11701 tttccgagag cttttttgta atggctgacg tccggtaatc cgacgctagt ctttcaacct
    11761 attccggcta tatgcaaaaa attccggctt tgtgcggggc ggcattgctt tgcctttccc
    11821 tcaacatttc ttatgcccaa gtggtggatt cggtatcagg ccgtctgttg aactaccctt
    11881 cacggttgtt tgccaggctg caatccaaat tatcggggct caacaacgat ctgggcactc
    11941 aaacgcagaa atacctggac aggatgtccc gccgcgaaca gcaattgcaa cgaaagctaa
    12001 tttctgtcga ttcggtcggt gctcagcggt tattcgccgg gtcggccgct cagtatgccg
    12061 cgttgatcca acggcttaaa tcagacacgg gcaaagctca tcaagctctc agcggacagt
    12121 atcggcccta c
//
LOCUS       DASYSV010000192         8270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1829645, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000192 DASYSV010000000
VERSION     DASYSV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829645"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="VGQ51_05865"
     CDS             complement(<1..1775)
                     /locus_tag="VGQ51_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEV9036123.1"
                     /translation="MSNQINKVSFANLVIALGIIYGDIGTSPLYVLNAIIHGRIIDEK
                     LILGSLSCIIWTLTLQTTVKYVWLTLKADNRGEGGIFSLYALVRRQKRWLVIPAMLGG
                     AALLADGMITPPISVTSAIEGLRQLPAFRFVEQWTIVYIVIGILVVLFFLQQFGTESI
                     GKLFGPIMFIWFSMMAVLGCSHLIDDWGIFKALSPHYAIELLTTYPKGFWILGAVFLC
                     TTGAEALYSDLGHCGRGNIRFSWIYVKACLILSYLGQGAFLLAQYKDRIFDTSVANPF
                     YSVMPSWFLMPGIIIATAAAIIASQALITGSFTLISESMRLNLWPKLRISYPTEERGQ
                     SYIPAINFMLFIGCSGITLYFQTSAHMEAAYGLAITLCMLATSILFSNYLISRRTSSP
                     LIYLYLAVYLTIEISFLTANLEKFPHGGFVTLIVGGGLFAVMFVWFRARKIKNRYVEF
                     VRMEHYIPQIQELSNDRSVAKAATHLVYMTSANNPKEIEHKIIYSILNKKPKRADIYW
                     FVHVDTMDDPYTAEYSVEHIIPNDIIRVEFRLGFRIEPKINLMFRKVVEDLVNNREVN
                     ITSRYESLERNNVVGDFQFIVMEKYL"
     gene            1920..3458
                     /locus_tag="VGQ51_05870"
     CDS             1920..3458
                     /locus_tag="VGQ51_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036124.1"
                     /translation="MSRILSCLLLILNLSALILAPHPAKAQVQQITYSEPEREDGRRT
                     NFEIIGKVNGNYLVFKNNSANSAISIYDSDMKLQSRIPLSFMPEKYINVDFVTYPDYC
                     YIIYEYQHRNIVYCTAVKLNGQGQELGKPIELDTTQVGFASNNKIYTTVVSEDKQRIM
                     VFKINSRNPHNFLFTTFLYDPQLNVLDRHRIFLPMEERNDLFTDFQLDNEGELVFGRY
                     MRSGSNDYISKVSVVTKGPTSDSFSIRDIGSGDRILDEIKIKVDNHNKRYILSGFFYK
                     QRRGNIEGLYTVIWDKATDSKVEENATVFTDDLRGQAKSSDDNIRMAFNDYFIKHIIT
                     KRDGGYLLVTESLYTTSRGNMFNRWDYMYNPAFGPGGFYSPYYYSPYYNPWSRNYGNY
                     ATRFHAENIMVLSFDAESNLQWSSVIPKSQFDDETENSISHELVNTGGELHFLFNLYE
                     RRNLLLNDQSISPEGKVTRYPTLRNLDRGYDFMPRYGKQVSSYESIIPCLYRNYLCFA
                     KVDF"
     gene            3575..4465
                     /locus_tag="VGQ51_05875"
     CDS             3575..4465
                     /locus_tag="VGQ51_05875"
                     /inference="COORDINATES: protein motif:HMM:NF040411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HEV9036125.1"
                     /translation="MPVLHREDNYIYRLILHVLDSAFHSFPVGWSILTFLLLITQATL
                     FDSVFNHFKVLPKPNFLPGMSYILITSLLPDWSHFSAPLLINTIMIWVWYRMMDLYNS
                     HRPGATIFNIGIWTGIVSLLYVPAVAFLLLVLSGLLTMRPFRVREWFVGLLGFTCPYY
                     FLFLVLYLSGHWKWADIAPSLVFNIPALPSSIWTAMGAAWLVIPFIIGGFFIQKNLSK
                     FLIQIRKSWSLLLLYLMVAIVIILILRVDSYDNWIILAVPLAAFHAAAYYYPTKTIGP
                     NILHWLIVIYILFMNYYPIS"
     gene            4541..5311
                     /gene="purQ"
                     /locus_tag="VGQ51_05880"
     CDS             4541..5311
                     /gene="purQ"
                     /locus_tag="VGQ51_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEV9036126.1"
                     /translation="MKFGVVVFPGSNCDRDMQDALQHDLGQEVVMLWHKDTALAGFGP
                     QDCIVLPGGFSYGDYLRCGAIARFSPIMQSISRFAAEGGKVFGVCNGFQILCESHLLP
                     GALLRNANQQFISRNIFLRGEGDNGKALKIPIAHGEGRYYADEKMLDELEADGQVIYR
                     YSDEQGRIVDAANPNGSLRNIAGICNKTRNVFGMMPHPERACSAALGNIDGKTILHTL
                     FIDAVAQPVIPASGSQSKMTPRRETANVGPSAGGPLDR"
     gene            5376..5831
                     /locus_tag="VGQ51_05885"
     CDS             5376..5831
                     /locus_tag="VGQ51_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036127.1"
                     /translation="MNKTALLIASLLAGTVAMAQSSKEVSWTYSAKKIAEKTYEIHMI
                     ATIGGDFHLYAQDAGGDGPIATTFTFTKSPLFTLDGTVKEKGQLVKKFESAWNHDVRY
                     YEKTVEFVQVVRLKSNVKTSLAGKVEFMVCNDHECLPPADVDIKVNIGG"
     gene            5824..7914
                     /locus_tag="VGQ51_05890"
     CDS             5824..7914
                     /locus_tag="VGQ51_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEV9036128.1"
                     /translation="MGRRLLYLASPPLLTLAVVLLVSSAVFAQNDSSQPPVSWNFSSQ
                     SGAIVLHAHIKDGWRLYSTTMPDSLVNSRIVLDSSAKAIITGIEEHGRLQTAYDTVLS
                     ATTRFFTDDVEWVVHLRPAAGPSTGTASPGGPSTGTSSPGGPSTGPSSPGGPSTGPSS
                     DLKGVINYMAEYGKDSIVTNGEQFRYGRTADGDLVAKSTALRSTGATLKLTTIDLANP
                     INKCGGTGAEGSKGLLSIFILGFLGGLLGLIMPCTFPMIPLTVSFFTKRATSRSKGIA
                     NAFLYGFFIFLIYVLISVPFYFLKQSDSNILNTISTSVPLNIIFGAIFIVFALSFFGL
                     FEITLPSRFSNSVDARSNITSVGGIFFMALTLAIVSFSCTGPILGSLLVGALNQTGGA
                     VQLTVAMAGFGLALGLPFALFALFPQWLKSLPRSGNWMNTVKIVFGFIEIALAVKYLS
                     NADLVVHKGLLKREVFVAVWLLIDIALVLFLFGIVRFHHDPPPARLSTGRKITGLFFI
                     AVGLYLAPGVTNTEYAKLPLLSGIAPPWNYSIYHRDFHLINDFEKAVQLAKQEHKPIL
                     IDFTGWACSNCRHMEEQVWPKPAVQNLIQNDYVLVSLYVDDRQALPEDQQFEFTSSDG
                     SKRAIRTIGDKFATMQTENFGSVSQPFYVLLSPDQRLLTRPVAYTPDPAAYAQWLQCG
                     LDAFKTVSSSSAKN"
BASE COUNT         1962 a         2417 c         1932 g         1959 t
ORIGIN      
        1 aggtatttct ccatgacgat gaactggaaa tccccgacca cattgttcct ttccaacgat
       61 tcataccggc tggtgatatt gacttcccgg ttattcacca ggtcctctac gactttacgg
      121 aacatcaggt tgatcttcgg ctcgatacgg aaacccagtc tgaactccac ccggataata
      181 tcattgggga tgatgtgctc tacggaatat tcggctgtgt atggatcatc catggtgtct
      241 acatggacaa accagtagat atcggcccgt ttgggctttt tattgagaat ggaataaatg
      301 atcttgtgtt ctatctcctt cggattgttg gcgctggtca tataaaccag gtgagtggcc
      361 gccttggcga cagaccggtc attgctcagc tcctgtatct gagggatata atgctccatc
      421 cggacaaatt caacgtagcg atttttgatc ttacgagccc tgaaccagac gaacatcacg
      481 gcaaacagcc ccccacccac gatcagcgtg acgaagcctc cgtgcgggaa tttctccagg
      541 ttggctgtca ggaagctgat ctcgatcgtg agataaacgg ccagataaag atagatcagc
      601 ggagagcttg tccgtctcga gatcagatag ttggaaaaaa ggatcgaggt agccagcata
      661 catagcgtga tggccagacc ataggccgct tccatatgtg ccgaggtttg aaaatataag
      721 gtgatcccgc tacagccgat gaataacata aaattgatcg cgggaatata ggactggccc
      781 cgttcttccg tcggatagct gatccgcagc ttgggccaga gattcagccg catgctttcg
      841 ctgatcagcg taaaggatcc tgtgatcagg gcctggctcg cgatgatggc ggcagccgta
      901 gcgatgatga tcccgggcat caggaaccac gacggcatga cgctgtagaa aggattggcg
      961 acggaggtat cgaatatcct gtccttatat tgtgccaata gaaaggcgcc ttgtcccaga
     1021 tagctgagga tcagacaggc ctttacatat atccatgaaa aacggatatt gcctctgccg
     1081 caatgtccga gatcggagta caatgcctcg gcccccgtcg tacacaggaa aactgcccca
     1141 aggatccaga accccttggg ataggtggtc agtaattcaa tagcatagtg agggctcagc
     1201 gccttgaaga tcccccaatc atcgatcaga tgagaacaac ccagcactgc catcatgctg
     1261 aaccaaatga acatgatcgg tccgaacagc ttcccgatcg actccgtccc aaactgctgc
     1321 agaaagaaca gtaccacgag gataccgatc acgatataaa cgatcgtcca ctgctctacg
     1381 aaacggaatg ccggcaactg ccggagacct tcgatcgctg aggtgaccga tatcggcggc
     1441 gtgatcatcc cgtccgcgag caatgcggct ccgccgagca tggccggtat cacgagccat
     1501 cttttctgtc gccttacaag agcatacaat gaaaaaatgc ccccctcccc cctgttgtca
     1561 gcctttaagg tcagccaaac gtatttgacg gtggtctgta gggtcagtgt ccagatgata
     1621 caggacaacg aacccaggat cagcttctcg tctatgattc gtccgtgaat aatggcgttt
     1681 aaaacgtaga gaggggaggt cccgatgtcg ccgtagatga tgcctaatgc tatgactaag
     1741 ttagcaaagc ttactttgtt gatctgattg ctcacgatgg cgccaaattt acaaccaaaa
     1801 ttcacatttc accagcctaa ttttacccgc cccgtcccat ccttccggca acccccggcc
     1861 aaaaaagccc gtacttttcc ctatcttaca tggcacattt aaaaacccga gctttcacga
     1921 tgagcagaat cctgtcctgc ctcctactga tacttaacct gtctgccctg atcctggctc
     1981 cgcatccggc aaaagcccag gttcagcaga tcacctattc cgaacccgaa agggaggacg
     2041 gccgccgcac caattttgaa attatcggaa aggtcaatgg taactatctg gtattcaaga
     2101 acaatagtgc caacagcgcc atcagcatct acgacagcga catgaagctc cagtcccgca
     2161 tacccctgag cttcatgccg gaaaaataca taaatgtgga tttcgtgacc taccccgact
     2221 actgttatat catctatgaa taccagcacc ggaacatcgt ctactgcacg gctgtcaaat
     2281 tgaacggcca ggggcaggag ctcggcaaac cgatcgagct cgatacgaca caggtcggct
     2341 tcgcctccaa taataagatc tataccaccg tcgtcagcga ggacaaacaa cggatcatgg
     2401 tcttcaagat caatagccga aacccccaca atttcctgtt caccaccttc ctctatgatc
     2461 cgcaattgaa tgtcctcgac cgtcaccgga tctttctgcc tatggaggaa aggaacgatc
     2521 tgttcaccga tttccagctg gacaatgaag gagagctggt cttcggcagg tatatgcgga
     2581 gcggtagcaa cgactatatt tccaaggtgt ccgtcgtcac caagggccca acttcggaca
     2641 gcttttcgat cagggatatc ggctccggcg accgcatcct cgatgagatc aagataaaag
     2701 tggacaacca caacaaacga tatatccttt ccgggttttt ctacaaacag cggcgtggca
     2761 atatcgaagg cctgtacacc gtcatctggg ataaggccac cgattcgaag gtggaggaaa
     2821 atgccacggt attcaccgat gacctgcggg gccaggccaa aagctccgat gacaatatcc
     2881 ggatggcttt caacgactat tttatcaaac atatcatcac caaaagggac ggaggctacc
     2941 tgctcgtgac cgagtcgctg tacacgactt cccgcgggaa tatgttcaac cgatgggact
     3001 atatgtacaa tcccgctttc ggccccgggg gcttctattc accatattat tacagccctt
     3061 attacaatcc ctggagccgc aactacggca actatgctac acgtttccat gcggaaaata
     3121 tcatggtgct ctccttcgac gccgagagca atctgcagtg gagcagcgtt atccccaaaa
     3181 gccagttcga cgatgaaacc gagaactcca tctcgcacga gctggtcaat acgggtggtg
     3241 agctccattt cctgttcaac ctgtacgaac gccgtaacct gctgctcaac gaccagagca
     3301 tctccccgga agggaaggtc acccgctatc cgaccctccg gaacctggat cgggggtacg
     3361 atttcatgcc gcgctatggc aaacaggtaa gcagctacga atcgataatt ccatgtcttt
     3421 atagaaatta tctttgtttt gcaaaagttg atttctaact aagaccacac cacctaccgt
     3481 gatcggcatt tttaaacaaa aaaccccggc aaacgcccta gtactcgttg tttacgcgct
     3541 ggtattaaaa ttttctctct ttcttcaccc ggtggtgccc gttttgcatc gggaggacaa
     3601 ctatatttac cggttgatcc tgcacgtcct cgactccgca tttcattcat tccccgtcgg
     3661 ctggtcgatc ctgaccttcc tcctcctgat aactcaggcc acgctcttcg acagcgtatt
     3721 caaccacttt aaggtcctgc cgaaacccaa ttttctgccc gggatgtcct atatcctgat
     3781 cacgtcgctg cttccggact ggagtcattt ctcggcccca ctgctcatca atacgatcat
     3841 gatctgggtc tggtaccgca tgatggacct ttacaatagc catcgccccg gcgctactat
     3901 cttcaatatc ggcatctgga cgggtatcgt atccctgctc tatgtccctg cagtagcctt
     3961 tctgctgctc gtgctcagcg gtctgctgac catgcgcccg ttccgtgtac gcgaatggtt
     4021 cgtcggcctt ctcggattca cctgccccta ctatttcctg ttcctcgtcc tgtatctctc
     4081 cggtcactgg aaatgggccg acatcgcacc atccctcgtg ttcaatatcc cggccctccc
     4141 gtcctccatc tggacggcca tgggcgccgc ctggctcgtc attcccttca tcatcggggg
     4201 gtttttcatc caaaagaatc ttagcaaatt cctcatccag atacgcaaga gctggagcct
     4261 tcttctgtta tacctcatgg tagccatcgt gatcatcctg atcctccggg tcgactccta
     4321 tgacaactgg atcatcctgg ccgtgcccct ggccgctttc cacgccgccg cctactatta
     4381 cccgacaaaa accatcggac ccaatatttt acattggctg atcgtcattt atatcttatt
     4441 catgaactat tatcctatct cgtgagggcc atcctctccg ccctccgact ttcccttccc
     4501 ggaaaatccg taacttgcac cctcaaacca atcgtcgtct atgaaattcg gagtagtcgt
     4561 ttttcccggt tccaactgtg atcgtgacat gcaggacgcc ctccaacacg acctcggcca
     4621 ggaagtcgtc atgctttggc ataaagacac tgcccttgct ggcttcggac cccaggactg
     4681 catcgtcctt cccggcggat tctcctatgg cgactatctc cgttgcggcg ctatcgcccg
     4741 attcagcccg atcatgcaaa gcatctcccg tttcgcagcc gaaggcggca aagtattcgg
     4801 cgtctgcaac ggcttccaga tcctctgcga gtcgcatctc ctccccgggg cattgctccg
     4861 gaatgccaac caacagttca tttccaggaa tattttcctg cgtggcgaag gcgacaacgg
     4921 caaggcgctg aagatcccca tcgcccacgg cgaaggacgc tattatgccg acgaaaaaat
     4981 gctcgatgag ctcgaggccg atggccaggt gatctatcgc tatagcgatg agcagggccg
     5041 catcgtagat gccgccaacc ccaacggcag cctgagaaat atagcaggta tttgcaataa
     5101 gacgcgcaac gtattcggaa tgatgcccca tcccgaacgc gcctgctctg cagcgctggg
     5161 taacatcgac ggcaaaacga tccttcatac cctgttcatc gatgccgtag cacagcccgt
     5221 gattcccgcc tcggggtcgc agtcgaaaat gacccctcgg cgggaaacag ctaatgtcgg
     5281 gccttcagcc ggcggcccct tggaccgcta gcggcaggcc aggctcgtaa aaatttagtt
     5341 ttctgtaaca ttccaaacca tatcttgcca ccgatatgaa caaaacagcc ctcctcatcg
     5401 catcccttct ggccggcacc gtcgccatgg cccaatccag caaagaagtc agctggacct
     5461 attccgcaaa aaaaattgcg gaaaagacct atgagataca tatgatcgcc acgatcggcg
     5521 gcgatttcca cctgtatgcg caggatgcgg gcggcgacgg accgatagca accactttca
     5581 cgtttaccaa aagcccactg ttcacgctcg atggcaccgt gaaggaaaaa ggccagctgg
     5641 tcaaaaaatt cgagtccgca tggaaccacg acgtacgata ttatgaaaag accgtggaat
     5701 tcgtccaggt cgtcagattg aaaagcaatg taaagacctc gctcgccggc aaagtggaat
     5761 tcatggtctg caatgatcat gaatgtcttc ctccggccga cgtcgatata aaagtgaata
     5821 tcggtgggta gacgtttact ttatttggct agtcccccgt tactgaccct ggcggttgtt
     5881 ctcctggttt catccgccgt cttcgcccag aacgattctt ctcagccacc ggtctcctgg
     5941 aacttctcct cgcaatccgg cgccatcgtc ctgcacgcgc atataaagga tggttggagg
     6001 ctctattcca cgaccatgcc ggatagcctg gtcaattccc gcatcgtcct cgatagctcg
     6061 gcaaaagcga tcatcaccgg tatcgaggaa catggccggt tgcaaaccgc ctatgacaca
     6121 gtcctcagtg caactacccg gttctttaca gacgacgtcg aatgggtcgt tcatcttcgt
     6181 cccgccgcag gcccttccac cggcaccgct tcgccgggcg gcccttccac aggcacctct
     6241 tccccgggcg gcccttccac cggcccctct tcccccggcg gcccttccac cggcccctct
     6301 tccgatctta aaggggtcat caactacatg gccgaatacg ggaaagactc catcgtcacc
     6361 aacggagaac aatttcgata tggcaggacc gccgacggcg acctcgtcgc caaatccacc
     6421 gcgctgcgat ccaccggcgc gacactaaag ctcaccacga tcgatctcgc caaccccatc
     6481 aataaatgcg gcggcacagg cgcagaaggt tccaaggggc tactcagcat atttatcctg
     6541 ggtttcctcg gcggactttt gggtttgatc atgccttgca cctttcctat gatcccgctg
     6601 accgtgagct tttttacgaa acgtgccact tcccggtcca aaggcatcgc caacgcgttt
     6661 ctctatggat tcttcatttt ccttatctat gtcctgatca gcgtgccttt ctatttcctg
     6721 aaacaaagcg actcgaatat cctgaataca atctcgacca gtgtcccgct caacatcatt
     6781 ttcggggcca tattcatcgt ttttgcgctc tcctttttcg ggctgttcga gattaccctt
     6841 cccagccgtt tttctaattc ggtggacgcg cgatcgaata tcaccagcgt cggcggcatc
     6901 tttttcatgg ccctgaccct tgccatcgtg tccttctcat gtactggccc gatcctgggt
     6961 tccctcctgg taggagcgct gaatcaaacc ggcggtgccg tacaactcac cgtggcgatg
     7021 gccggcttcg ggctggcgtt gggactgcct tttgcgcttt tcgccctgtt ccctcaatgg
     7081 ctcaagagcc tgccccgctc aggtaactgg atgaatacgg tcaagatcgt cttcgggttc
     7141 atcgagatcg cgctggcggt aaagtacctg tccaatgccg acctcgtcgt acacaaggga
     7201 ctgctcaaac gcgaggtctt cgtcgcggtc tggctgctga tcgacatcgc gctggtgctc
     7261 tttcttttcg ggattgtccg gttccaccat gaccccccgc cggccagatt gtcgacaggc
     7321 cggaagatca cagggttgtt ctttatagcc gtcggcctct acctggcacc cggagtcact
     7381 aacacggaat atgccaagct gccgcttctt agcgggatcg cgccgccctg gaattacagc
     7441 atctatcatc gggatttcca cctgatcaat gatttcgaaa aagccgttca gctggcgaag
     7501 caggagcata aacccatcct catcgatttt accggatggg cctgttcgaa ttgccgccat
     7561 atggaggaac aggtatggcc aaagcccgcc gttcagaatt tgatccagaa tgactatgtc
     7621 ctggtctccc tatatgtcga cgaccgtcag gccttacccg aggatcagca attcgaattc
     7681 accagtagtg acggtagtaa aagggcgatc cgtactatcg gagataaatt cgcgacgatg
     7741 caaacggaga attttggtag cgtctcacag ccgttctacg tgttgctcag ccccgaccag
     7801 cgacttctga cacgaccggt cgcctacacg ccggaccctg ccgcctacgc ccaatggctg
     7861 caatgtggtc tggatgcctt caagacagtc tcttcttcgt ccgcaaaaaa ttagcgaaca
     7921 tgcaacggct gcccgcactc cattgtcaaa cgggtaagat ccgtatcaga taataagaaa
     7981 aggccgactc tttacagagc cggcctttta ataaaaggtg aggcccgccg aaggcgggcc
     8041 tcattaatta ggcgtcattc tgtcccggcc ttccggcaag actaagggca agtgcttaac
     8101 accagatgga gagccagaat gacaagaaaa taaaaaattc ccactcgaat gagcgggaat
     8161 tttttaatat tttttttaat gcatcttttc acacaatgaa gttagcggtt ctgtccgttc
     8221 cccgttctta aaggtagtta aagattatta aaaaaatccg tccatcgtat
//
LOCUS       DASYSV010000193        17550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_418296, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000193 DASYSV010000000
VERSION     DASYSV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 17550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17550
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418296"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..589
                     /locus_tag="VGQ51_05895"
     CDS             311..589
                     /locus_tag="VGQ51_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV9036129.1"
                     /translation="MRQRQLSCSALWSFFTPYGEITSARIINDKATGLSRGFGFVEMK
                     DEAAARKAIAELNKATVDGRSIKVLGARPRTEMSFRSNSGGGSRNNRW"
     gene            605..1051
                     /locus_tag="VGQ51_05900"
     CDS             605..1051
                     /locus_tag="VGQ51_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEV9036130.1"
                     /translation="MAKSKETFNKKEKEKQRQKAKREKRDKMEERRTSQAKGKSLDEM
                     LAYVDENGYITSTPPDPKKRKRVNAEDIEIGVPKRVEVEDPLKEGKIDYFDGTKGFGF
                     ILQNDGTKVFFHINQTTYPVREGDIVNYTVERGPKGWNAANVTKKP"
     gene            1507..1710
                     /locus_tag="VGQ51_05905"
     CDS             1507..1710
                     /locus_tag="VGQ51_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036131.1"
                     /translation="MAELVGKGIDPNLPLSIWLCFSDCMNNRGTSSLFIDFDPTPEGT
                     VGQIVRFLHDPDSFRVIADSFEN"
     gene            complement(2146..2217)
                     /locus_tag="VGQ51_05910"
     tRNA            complement(2146..2217)
                     /locus_tag="VGQ51_05910"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2182..2184),aa:Glu,seq:ttc)
     gene            2566..3579
                     /gene="gap"
                     /locus_tag="VGQ51_05915"
     CDS             2566..3579
                     /gene="gap"
                     /locus_tag="VGQ51_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV9036132.1"
                     /translation="MSTVKVAINGFGRIGRLAFRQIHGLEGIEVVAINDLTSPKVLAH
                     LLKYDSAQGRFAEEVKATENSIVVGGDEIKIYAHKNPAEIPWKGHNVDVVLECTGFFT
                     DKEKAEAHLKAGAKRVVISAPATGDLKTIVFNVNHNILDGSETIISCASCTTNCLAPM
                     AQVLDDKFGIVTGLMTTIHAYTNDQNTQDAPHPKGDLRRARAAAVNIVPNSTGAAKAI
                     GLVLPKLKGKLDGGAQRVPTLTGSLTELVTILNKKVTLEEVNAAMKAASNESFGYTED
                     EIVSSDIIGISYGSLYDATQTRVITGGDQQLVKTVSWYDNEMSYVSQLVRTLYFFAKM
                     ISK"
     gene            3628..4821
                     /locus_tag="VGQ51_05920"
     CDS             3628..4821
                     /locus_tag="VGQ51_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV9036133.1"
                     /translation="MSQFSQHNFANEKALVRVDFNVPLDDKYNITDDTRIRAAIPTIK
                     KILADGGKAILMSHLGRPKDGPTEKYSLNHLVPHLRKLLKTPIIFAEDCIGSPAEKAI
                     AKAQPGQVVLLENLRFYKEEEKGDKAFAERLAKLGDVYVNDAFGTAHRAHASTAVIAQ
                     FFPKDKRMFGLLMEGEVSSAEKVLHEAQKPFTAIIGGAKVSDKILIIENLLERATDII
                     IGGGMAYTFLKALGGNIGNSLCEDDRLDTARDLCSKADAKGVRIHLPSDSIIADKFDA
                     NANDSTAPSNAIPDGWMGLDIGENACEQFTNVIHRSKTILWNGPMGVFEMAKFQRGTK
                     GIARAVAEATEKGAFSLVGGGDSVAAVNQFGYADKVSYVSTGGGAMLEYFEGKELPGI
                     AAVNA"
     gene            4873..6690
                     /locus_tag="VGQ51_05925"
     CDS             4873..6690
                     /locus_tag="VGQ51_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV9036134.1"
                     /translation="MRRTCLFLALCLLNTVLFAQKKHAPSDPFSGLDTAFARVLKDWH
                     AVGFAVAVVDKDKVIYSNGFGYKDWENKVPVTPHTLFAIGSCTKAFTASLIGMLDEKG
                     TLDIDKPVRNYLPELKFYNNTMNDNITLRDMMCHRTGLPRHDFAWYLFSGDSRDSMIR
                     KVQYQEPSAGIREKWQYNNFMFVAQGVVVEKLSGKSWEENIKEKILQPLGMNETDLSI
                     NDMIRTQDFSRGYGVIRDAFIHKEDYFVIDAMGPAGSINSNVLDMANWVTTWINGGKF
                     NGKQILPAKYVNEAISSQMVMGAGLPAKQSPDVLFATYGFGWMLDVYRGHYRVEHGGN
                     IDGFSANTCFFPSDSIGIIVLSNQSGSAVPSIVRNLVADRLLHLPYKDWDTYLKDIAD
                     KAKRDIRSAEAAQVVDHNPAPGPSHPLTDYTGIYSNPGYGSFEVAMVKDSLTILFPRY
                     KGWLRHQRYDIFQSFEKDAKYGIDTSGTGGPKIQFNMDLAGNISGLSANLEPSLANPI
                     VFIRTPKAKAVSAEILQKYVGDYIIGPVTIKVYIKGGLLYFFVPGQPEYELANVGVDK
                     FALKPLNGYFAQFVLDDQGQVNALLAIQPNGTFKATKKK"
     gene            complement(6758..7747)
                     /locus_tag="VGQ51_05930"
     CDS             complement(6758..7747)
                     /locus_tag="VGQ51_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV9036135.1"
                     /translation="MNALVIKEKNVPPVWTSVTEPVAGAGEAIVRVHAAALNHRDVWI
                     QKGQYAGLKYPIIPGSDGSGVVMPGPDAGAWADKEVIINPGMHWGKHAGFQDPEDFRI
                     LGLPDDGTLGEYVRVPVENLVRKPAHLSFEEAAALPLAGLTAYRALMTRARLQAGETV
                     LITGIGGGVALFALQFAVAEKAKVYVSSGSDQKIDRAMSMGAIGGANYKNPGWTGALR
                     SQGFDVVVDGAAGDGLNDLLDLLAPGGRLAIYGATRGNVSGVVARRIFWKQLNILGST
                     MGSPTDFAAMVALVDQHKIKPVVDRVYAPEDGAAAFQRMDEGLQFGKIVVKMR"
     gene            7903..10278
                     /locus_tag="VGQ51_05935"
     CDS             7903..10278
                     /locus_tag="VGQ51_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036136.1"
                     /translation="MLRSYLAIFLRGFRKKPVYSILNITGLALGIACAALIFLWVEDE
                     LSFDHSYARRDQLYGVMMNMEYSNKIETNSSIPGPMADAVRAVIPDVVNISRFGFGQE
                     LFAINDKAAYENGLYVDTGFFSMMQPEFIKGNAAGFTNLHSIVISATMATKFFGSADP
                     IGRTLRVDNTQEFMVIGVVKDAPSNVSLRYGWLAPVQNFLDKNRWLKSWDTYGISTLV
                     QLRDDADAGKVSNQLTALLRPKSKIYTHASCLLQSIKDWRLRSRFTNGHQDGGAITYV
                     HLMSAIAWIIIFIACINFMNLATARAGQRAREVGVRKTLGAVRKALIGQFIFESLLMS
                     FLAVALAIILVYIALPGFNTLVGKQLPFVPFAPVHLAALLTIGILCGLIAGSYPAFYL
                     SSFQPVAVLKGLRIKVNTGAGFIRKGLVITQFTVSVTLIVCTVIIYQQVQYARSRDLG
                     FDKEHLISISLRGDLAQHFDAVRTELMQTGLVANASLSASPPLSMWETVTSQEITWAG
                     ADAGKGVRICWEHVSPEYFSTMGLQIKRGRNFYPDAKVDSNNMIINGAMAELMGKEGR
                     VGGYLSDGIRAPYRIVGIVKNFLFNDMHASSVAPLMLSCGPVRPGMFNFMEVRLKAGN
                     DLPTALTKVETILKANNPGYPVDYRWTDQDFEGSFSEEAHLGQFAGIFSMLAIFISCL
                     GLFGLAAYTAERRTKEIGIRKILGASTTTLASLLSKEFLQLVTLSCLIAFPLAWWVMN
                     GWLKDYSYRITIRWWVFALSGAAAVSIALLTVSFLAIRTARTNPVKSLRTD"
     gene            complement(10372..11727)
                     /locus_tag="VGQ51_05940"
     CDS             complement(10372..11727)
                     /locus_tag="VGQ51_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9036137.1"
                     /translation="MTTSRRDFIRKSSMAGVAGYMGALSFPASSYRRIPGANDRVRVG
                     VVGFSDRHRSSHVPCFMNHYKELNFDVVAVSDIWKMRREQGAALWKDKMQHDIVACRN
                     NEELYSKKLVDAVFVSTADFQHARHAIEAVKAGCDAYVEKPFAETMEDNRDALKAIKA
                     TDRIVQIGSQRRSGANYHAANEFIRSGKFGPITMVELTWNVNQPGRWRRPSLVGQLKE
                     EDTDWNRWLLNRPHDTFDPRKYLEFRLFWPYSSGLPGQWMSHQIDTVHWFSGLKHPRS
                     VVANGGIYVWKDGRKNWDTITAVFDYGPDDDPSSGFQVTFASRMHNGEEKPAEIYYSN
                     GGELNLITNKVSPTGGLTKHFADQMHMQPNLLPEMSLSEAEPVVASADTGGDKLTSNH
                     IRNWMECIRSRKTPNAPVEAGYSHSIANIMTNAAVHTGHKAYFDEKTQEVMAGGKVFK
                     Y"
     gene            11795..12976
                     /locus_tag="VGQ51_05945"
     CDS             11795..12976
                     /locus_tag="VGQ51_05945"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HEV9036138.1"
                     /translation="MITLRLLILRKRFRWTSMLSTPSTPSIPSIPRIASIAIPILLAL
                     AGLALVPGTLSAQQLPIGVSVDAADRRVVVTAGGQPFTTFIYPDSLEKPVLYPIYAPD
                     GQLITRGFPVSPRPGEPVDHPHHLGLWFNYEKVNGLDFWNNSYAIPAAKKSQYGWIHT
                     DSINGHSAASLPPSNSVMLGRNDLRYKARWTDQQGHVLLDEATHFFFSATRDERIIDR
                     ITTLTAVSDGGVSFPDAKDGMLGLRVTKELQIPSNAPGQFVDDKGNITKVAAGNTPDI
                     NGNYLTSEGKQGDSAWGTRGTWCLLYGKKNNDMLSIAIIDHPGNPGYPTYWHARGYGL
                     FAANPLGQSIFSNGRETMNFRLAKGQSATFRYRIVIAAGKDRLSTGRIRQLADAFAHI
                     E"
     gene            13082..13672
                     /locus_tag="VGQ51_05950"
     CDS             13082..13672
                     /locus_tag="VGQ51_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV9036139.1"
                     /translation="MSTRNLSLIIIALIILVLGGCGCGGYNNMVRLDENVKSSWGKVQ
                     SDYQRRNDLIPNLVNTVKGASNFEQQTLTNVIEARAKATSVQIDANNLTPEKIAQFQQ
                     AQGQVSSSLSRLLAVVENYPDLKATQNYRDLQAQLEGTENRIKVSRNDFNDAVQQYNS
                     TVRRFPNNIFAGMFGFATKGYFQSEPGSEKAPEVKF"
     gene            13809..14267
                     /locus_tag="VGQ51_05955"
     CDS             13809..14267
                     /locus_tag="VGQ51_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEV9036140.1"
                     /translation="MSLSLFKRKPLFNEDQQQEITTAIRDAEKCTSGEIRVFIERHCR
                     YMDPLDRALEVFAALQMERTAARNGVLVYVALKDRQLAVFGDRGIHEKVGVVFWNQQV
                     KTILSHFNNADYSHGIAAVVTEIGKALQQHFPYDPDTDKNELPDDIVFGR"
     gene            14333..15148
                     /locus_tag="VGQ51_05960"
     CDS             14333..15148
                     /locus_tag="VGQ51_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEV9036141.1"
                     /translation="MKKLLGILILAPCLLAVNLTRAQDVPPRPNPATAVNDFAHVMTP
                     DQVSALEQKLIAYNDSTTSQIVIVTVPDIGDAAIEDYALKILRSWGVGDKKNNNGVVI
                     LADIKDHKVYIAVGYGLEGAIPDATAKEIVDNEIVPNFRAGGDDNYYRGFDQAADALI
                     KAAAGEYKAPAGYGQRGRRHGGSGLGVFIIIFIVIIIIISRRGGGGRGGGGYYGGGGW
                     LPFILGTMVGRGLGGGGGGWGSGGGGGWSGGDGGFGGGFGGGSGGGGGAGGSW"
     gene            complement(15275..>17550)
                     /locus_tag="VGQ51_05965"
     CDS             complement(15275..>17550)
                     /locus_tag="VGQ51_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV9036142.1"
                     /translation="TDTVTLDAKGMDIHSIAIDRAGKKTPLKYDYDGMQVTIHLDKTY
                     TRSEKYTLFIDYTAKPNELKVHQGSAAITDARGLYFINPKGEDKTKPTEIWTQGETEA
                     NSVWFPTIDKPNEKTTEEIYMTVPAKYVTLSNGLLVDQNTNGDGTRTDHWKLDEPISP
                     YLFFMGVGDYAIVKDSYKGKEVAYYVEKEYAPVARRIFGHTPEMIAFYSRITGVDYPW
                     PKYDQITGRDYVSGAMENATATLHSDAAQQDARELTDGNQWEDVIAHELFHHWFGDLV
                     TCESWSNITLNESFADYSETLWNEYKYGKDAGEAVNFSRIQSYLQQPDNAKKDLVRYY
                     YADKEDVFDGVSYPKGGRILNMLRNYVGDSAFFKALHLYLTDHKFGNAEAADLRLAFE
                     AVTGEDLHWYWDQWYYGSGHPKLAIDYVYDDAAGKVQVIVNQTQETGKVFRLPIAIDV
                     YNGPAKERHKVWIEDKTDTFTFAYKSHPDLVNVDGDKVLLCEKKDNKTLANFVFQYKY
                     AGNYVDRREAIEYCAKHQDDPKAIDLLKTAMRDRYYRLRNLALTRIDLSHDAVRSAFE
                     PLILEIAEHDPNHPVKAKAIELLGTYKNASYKALFLKATTDSSYSVAGNALTALAAID
                     STQALDVAKKLVKEPAKGGLTVAIAGVLIKSGDESNFDAISENFEKMPVGQAKFNFSM
                     AYANMLGKVSNTEKLKHGVDLLIEFKEAIPSAYRGQTDPVFDNMLQGVAKKRQAAGSQ
                     DQADYITSRLSNEKKGF"
BASE COUNT         4135 a         4907 c         4527 g         3981 t
ORIGIN      
        1 ttcagccaat tcaaggagaa tgcgtttgtc ctgatcgaca aaggcgattt ggaggtcgac
       61 ctgctgccct ttggagccgt cgacgaggaa gacggctggg taggaagacg gatcaaagcg
      121 catcgcggat aatgccttgg tcgcactgac tttgatgatt gccgaaagca aaccgaatga
      181 aaaggacatt atgactcaag tcgtcgtcaa cctcaacaat ggatgtaact cctaaaaagt
      241 ctgtaaattt tttatctaca agcgttgccg aaataaaggc ctatcttcac aggattactt
      301 taggcctcgc atccggcaac ggcaattatc ctgctctgca ctatggagtt tttttacccc
      361 ttacggcgag attacctcag ccaggatcat caatgacaag gcaacaggtc tatcgagggg
      421 attcggtttc gtcgagatga aagacgaggc ggcggccagg aaggccattg ctgagcttaa
      481 caaagccacg gtcgatggcc gcagtattaa agtgctgggg gccagaccca ggacagaaat
      541 gtctttccgt agcaatagtg gcggcggatc caggaacaat cggtggtaat taaaacggaa
      601 aactatggcg aaatcgaaag aaacctttaa caagaaagaa aaagaaaagc agcgtcaaaa
      661 ggcaaagcgg gaaaagcgcg ataaaatgga agaacgccgg accagccagg cgaaaggcaa
      721 gtctttggat gaaatgctgg cctatgtgga cgagaacggc tacatcactt ccaccccgcc
      781 tgaccccaaa aaaagaaaac gggtcaacgc ggaggatata gaaatcggcg tgccaaaaag
      841 ggttgaggtc gaagatccgc tcaaagaagg taagatcgac tattttgacg ggacaaaagg
      901 ttttggcttt atacttcaaa atgatggtac aaaggtgttt tttcatatta accagactac
      961 ctaccccgtt agggaaggtg atatcgtgaa ctacaccgtc gaaaggggac caaaaggctg
     1021 gaacgccgcg aacgttacca aaaagccctg accggctccg ccgcaacaag attatttatc
     1081 caatatgctg gttgtatgtt taaaggctcg tccattgacg accttcttta tcctccttct
     1141 tcgcacttta atgaccccaa tggggcaatt atcgacccaa catcaaacag atctggtcgg
     1201 cgacgttttc ccgcattaat attttcagat cgcggaagaa ctggttctcg tttgtcagcc
     1261 tcccgatgct gatgggaatg cttcctttct tctttctgtt ccggtatttc tcccggtatt
     1321 tgccgatcgc tgttgattgc gaaggccacc ttattatcta actggaacta ttacaaatcc
     1381 ttgtttagcc aatctcctga gaagaggtta gtgtacttca gcccgcggta agtcggtgga
     1441 gcagattccg accaataatc cttacacgca atcgatccag cagatttatg cgaaatactt
     1501 cgacgaatgg ccgagctggt agggaagggg attgatccga acctgccgct gtctatttgg
     1561 ctttgttttt ctgattgcat gaataataga gggacctcca gtctcttcat tgattttgac
     1621 ccgacgccgg aaggaactgt cgggcaaatt gttcgtttct tgcatgaccc ggacagtttc
     1681 agagttatcg ccgattcatt tgaaaattaa ttgcagaaat tgatcgatga aaattttagc
     1741 tttcttgcga aggaagaata agaattagtt gcttgccagg agatgactta agttcaaacc
     1801 acttgaatgc agaccaaaat gtggcacggc aaaattatga attgggaaat aacctgaact
     1861 ttgcaacatt cattacgaat aaagaaggcc atctcgattt gagatggcct tcaattttag
     1921 gtgccacata aggtcatcgc tgatcaggat gtaaagatca atgggagcca acgaatagtg
     1981 caatctatat taaatgagaa agaagaagat ctgctgaaat cggaggatcg ctaattgaag
     2041 acacagctcg ctgtgaaaaa tcttaggtcg accgaagaag attctgatat tcagtaaata
     2101 aaaagcccca atcataaaca attggggctt actgacaaat ttaggtaccg cgtaggggaa
     2161 tcgaacccct cattcctccg tgaaagggag gcgtcttaac cgattgacca acgcggcgtt
     2221 ggattgaacc gtattgccgg ttttcctcga aaggagtgca aagatatagg cgtttgaaac
     2281 gccggccaaa tttttcggac tcttcctttc aattacagca agatacgtgg cggcatggtt
     2341 ttttcgatcc gcgcgagagg ccgcggagag atgcagccgg cgtcacgggt ggtgacgtgg
     2401 cgccgtcgct tcccattatc tccaacgctc aaccgccccc gtcaatccct tggttataaa
     2461 aaaattaggc cgtcttttca attgatatcc aaaaaattac aatcatttag gtctcaaaaa
     2521 cgtaacttcg ctacctcaaa atttaaaatt caaattcatt cgtctatgag cacagttaaa
     2581 gttgctatca atggttttgg ccggatcgga cgtttggctt tccgccagat ccacggtctg
     2641 gaaggaatag aagtagtggc gattaatgac ctcacgagtc ccaaagttct ggctcacctg
     2701 ctgaaatatg acagcgcaca gggccgtttc gcggaagaag taaaggctac cgagaactcc
     2761 atcgtcgtag gcggcgatga gataaaaata tacgcacata agaacccggc cgaaattccc
     2821 tggaaaggcc ataatgtcga cgtcgtcctg gaatgcaccg gattcttcac cgataaagag
     2881 aaggccgaag cccacttaaa agccggcgca aaacgcgtcg tgatctccgc accggcaaca
     2941 ggcgatctga agaccatcgt tttcaacgtc aaccacaaca tcctggacgg cagcgaaacg
     3001 atcatcagct gcgcctcctg taccaccaac tgtctggcgc ccatggccca ggtcctcgat
     3061 gataagttcg gcatcgtcac gggtctcatg accacgatcc acgcttatac caacgaccag
     3121 aatacgcagg acgcgccgca tcccaaaggc gatctccgcc gcgcccgcgc agccgcagtc
     3181 aatatcgttc ccaacagcac cggcgccgcc aaggctatcg gactggtcct tcccaaactg
     3241 aaaggtaagc tcgacggggg cgcccagcgc gtaccgacgc tcaccggaag tctcaccgag
     3301 ctggttacga tcctcaataa aaaggtcacc ctcgaagaag tgaatgccgc catgaaagcc
     3361 gcatccaacg aaagcttcgg gtacaccgag gacgagatcg tcagcagtga tatcatcggc
     3421 atctcctacg gaagcctgta cgacgccacg caaacgcgcg tcatcacggg tggcgaccaa
     3481 cagctggtaa agaccgtctc gtggtatgac aacgagatga gctatgtcag ccagctcgtc
     3541 cgcaccctct acttcttcgc aaagatgatc tccaaataag attgctcccc cggcccggct
     3601 actcgccggg cctttttatc tatacttatg agccagttca gccagcataa ttttgccaac
     3661 gaaaaagcac tcgtccgcgt cgacttcaac gttccgctcg acgacaaata caatatcacc
     3721 gacgacaccc gtatccgtgc ggccatccca acgatcaaaa agatccttgc cgatggtgga
     3781 aaagcgatcc tgatgagcca tctcggccgg cctaaagacg ggcccaccga gaaatattcg
     3841 ctgaatcatc tggtgccaca ccttcgcaaa ctactaaaga cacccatcat cttcgccgaa
     3901 gactgtatcg gctcgccggc ggaaaaggcc atcgcaaagg cacagcccgg tcaggtagtg
     3961 ctgctggaaa atctccgctt ctataaagaa gaggaaaaag gcgataaggc cttcgccgaa
     4021 agactggcga aactcgggga tgtctatgtc aacgacgcct ttggcactgc ccaccgcgct
     4081 cacgcttcga cagccgtcat cgcccagttc ttccccaagg acaaacgcat gttcggtctc
     4141 ctcatggaag gggaggtgag cagcgcggaa aaggtcctgc acgaggcgca gaaacccttc
     4201 accgccatca tcggcggggc caaggtctcc gacaagatcc tcatcatcga aaacctgctc
     4261 gaacgcgcca ccgatatcat catcggcggc ggtatggctt atactttttt gaaggctctc
     4321 ggcggcaata tcggcaactc gctctgtgaa gacgatcgtc tcgacacggc ccgggacttg
     4381 tgcagcaagg ccgacgccaa aggcgtccgt atccacctgc cctccgactc catcatcgcc
     4441 gataagttcg acgccaatgc caacgattcc acggccccca gcaacgcgat ccccgacgga
     4501 tggatggggc tcgatatcgg ggagaatgcg tgcgagcagt tcaccaatgt cattcatcgc
     4561 agcaaaacga tcctttggaa cggcccgatg ggagtattcg aaatggccaa attccagcgc
     4621 ggcaccaaag gtatcgccag ggcggtggcg gaagcaaccg aaaaaggcgc tttctcgctg
     4681 gtaggcggcg gcgattccgt ggcggcggtc aaccaattcg gttatgccga caaggtcagc
     4741 tatgtctcca ccggcggcgg cgccatgctc gaatatttcg aagggaagga actgccgggg
     4801 atagccgcag tgaatgcata gattggccgt gtgcacgata aatcttagat ttatcgtgcc
     4861 aaaccttcta tcatgcgacg cacctgtctc tttctcgccc tttgccttct aaacacagtc
     4921 ctcttcgctc aaaaaaagca cgctccttcg gaccctttct ccggtctcga caccgccttt
     4981 gcacgcgttt tgaaagactg gcatgccgtc ggatttgccg tggccgtcgt cgacaaagac
     5041 aaggtcatct actccaatgg attcggatat aaggattggg aaaacaaggt cccggtaacg
     5101 ccgcatacgc tttttgcgat agggtcctgc accaaggctt tcaccgcgtc gctgatcggc
     5161 atgctcgacg agaaaggtac cctcgacatc gacaagcctg tccgcaacta tcttcccgag
     5221 ctgaagttct acaataacac gatgaacgac aacatcacgc tccgcgatat gatgtgtcac
     5281 cgtaccgggc tcccccgtca tgatttcgcg tggtaccttt tcagcggcga cagccgcgac
     5341 tcgatgatcc gaaaggtcca ataccaggag cccagcgcgg gcatacgcga gaaatggcag
     5401 tataacaatt tcatgttcgt cgcccagggc gtagtggtgg aaaagctgtc cggcaaatcc
     5461 tgggaggaaa atatcaaaga gaagatactg caacccctgg gaatgaacga aaccgatctg
     5521 tccatcaacg atatgatcag gacgcaggat ttctcccgcg gttatggggt gatcagggac
     5581 gcattcatcc ataaggagga ctattttgtc atcgacgcca tgggtccggc cggcagcata
     5641 aacagcaatg tgctcgacat ggccaactgg gtgaccacct ggatcaatgg cggcaaattc
     5701 aatggcaaac agatccttcc tgccaaatat gtgaacgaag ccatcagcag ccagatggtc
     5761 atgggagccg ggttgcctgc gaaacagtct ccggatgtgc tgttcgctac ctatgggttt
     5821 ggctggatgc tcgatgttta ccgcggccat taccgtgtag aacacggcgg caatatcgac
     5881 ggcttttccg ccaatacctg tttcttcccc tccgacagca taggcatcat cgtactcagc
     5941 aatcaaagcg ggtcagcagt gccttctatc gtccggaacc ttgtcgccga ccgccttctg
     6001 catcttccgt ataaagactg ggacacctat ctgaaagata tcgccgacaa ggctaaacgc
     6061 gacatcagga gcgccgaagc cgcgcaggtc gtcgatcata atccagcacc cgggccctcg
     6121 catccgctga ccgactatac cggcatctat tcgaatcccg gctatggcag ctttgaagta
     6181 gccatggtga aggactctct cacgatcctt ttcccacggt ataaggggtg gctgagacac
     6241 cagcggtacg acatcttcca gtcgtttgaa aaagatgcga agtatggtat cgacacctca
     6301 ggtacgggtg ggccgaagat ccaattcaac atggatctcg cggggaatat cagcggcctg
     6361 tcggccaatc tggagccttc ccttgccaat cccatcgtat tcatccgcac ccccaaggcg
     6421 aaggccgtca gtgccgaaat cttgcagaaa tatgtagggg attatataat agggcccgtc
     6481 acgataaaag tatatataaa aggagggttg ttgtattttt tcgtgcccgg tcagccggag
     6541 tatgaattgg ctaatgtcgg cgtggataaa ttcgcactca aaccgctgaa cggatatttt
     6601 gcacagtttg tgttggacga tcaaggccag gtgaatgcgc tgctcgcgat ccagcccaac
     6661 gggacattca aggctacgaa gaaaaagtag gtgacggcga cggcaaagtc ctccgcccgc
     6721 aggagcccgc gtgccgcgag cggcgcaggc cctcgcgtca tcgcatcttc accactatct
     6781 ttccgaactg cagcccttca tccatccgct ggaaagccgc agcaccgtcc tccggcgcat
     6841 ataccctgtc tacgacaggt tttatcttat gctggtcgac cagcgccacc atggcggcaa
     6901 aatccgtggg gcttcccatc gtcgatccga gtatattcag ttgtttccaa aaaatgcgtc
     6961 gcgccacgac accggagaca tttccccgtg tcgcgccata gattgccaga cgccctcccg
     7021 gagcgagcag atccagcaga tcgttgagac cgtctcccgc ggcgccgtcc acgacgacat
     7081 cgaaaccctg cgaccgcaat gccccggtcc accctggatt tttgtagtta gcgccgccaa
     7141 tcgcgcccat ggacatcgcc cgatcgatct tttgatcgct gcccgagctc acatacacct
     7201 tcgccttctc cgccaccgca aactgcagcg caaacaaggc cactcctccg ccgatacccg
     7261 tgattagtac cgtctcgcca gcctgcagcc gagcccgcgt catcaatgcg cggtatgccg
     7321 tcagacccgc taaaggcaat gccgcggctt cttcgaatga cagatgcgcg ggcttacgca
     7381 ccaaattttc caccggaacg cgaacatatt ctccgagcgt tccgtcatcc ggcagtccca
     7441 ggatgcgaaa gtcttcgggg tcctggaacc ctgcatgttt tccccaatgc atcccagggt
     7501 tgatgatgac ttctttgtcg gcccatgcgc ccgcatccgg ccccggcatg acgactcccg
     7561 acccatccga gcccgggatg atgggatatt tcaaacccgc atattgtcct ttctggatcc
     7621 atacgtcgcg gtgattcaat gccgccgcat gcactcgcac gatagcctcc ccggcgccag
     7681 ccaccggctc cgtaaccgat gtccacaccg gcggcacatt cttctcttta atgaccaatg
     7741 cattcataga tggtaagtta aatacttcgc ccgatcgcga acacaaaagt atcgaaaccg
     7801 gtcacagcca caatcataaa taaatataac atcttaaaaa ccagcggttt cataaattgg
     7861 cacacatctg ggatctctaa aacaccgaat cctccaaaat atatgctaag aagttatctc
     7921 gccatatttc ttcgcggttt tcgcaaaaaa cctgtttaca gcatactcaa catcactggt
     7981 cttgccctcg gtatcgcctg tgctgctctc atattcctat gggtcgaaga tgaattgagt
     8041 ttcgaccatt catacgcgag acgcgatcag ctatatgggg tcatgatgaa tatggagtat
     8101 tccaataaga tcgaaaccaa ttcttctata cccggcccca tggcagatgc cgtccgggcc
     8161 gttatacccg acgtcgtcaa tatctcgcgt tttggtttcg gccaggaact ctttgccatt
     8221 aacgacaaag ccgcctatga gaacggacta tatgtggaca cgggtttctt ctctatgatg
     8281 cagccggaat ttataaaagg caatgccgcc ggcttcacca atctccattc catagtcatt
     8341 tctgctacga tggctacgaa gtttttcggt tcggcagatc cgataggcag gaccctccgt
     8401 gtcgacaaca ctcaggaatt tatggtcatc ggggtcgtca aagatgcgcc gtccaatgtc
     8461 tcgcttcgct atgggtggct ggcgccggta caaaacttct tagataagaa caggtggctc
     8521 aaatcatggg acacctatgg catttccaca ctcgtacaat tgcgggatga cgccgacgcc
     8581 ggcaaggtaa gcaaccagct gacggccttg ctgcggccca aatcgaagat atatacgcac
     8641 gcaagctgtt tgcttcagtc gataaaggat tggcgcctgc gcagtcgttt taccaatggc
     8701 catcaggatg ggggcgctat tacttacgtt cacctcatgt ccgcgatcgc ctggatcatc
     8761 atcttcatcg cctgcatcaa ttttatgaat ctcgcgactg cccgggcggg acaacgtgcc
     8821 cgcgaagtag gggtccggaa aaccctgggt gcagtgcgca aagcgctcat cggacaattc
     8881 atcttcgaat ccctcctgat gtcctttctg gccgtagcgt tggcgataat cctggtatac
     8941 atcgccctgc cgggcttcaa caccctcgtc ggaaagcagt tgccattcgt tccctttgct
     9001 cccgtccatc ttgccgcgct gctcactatc ggcatccttt gcggtcttat cgccggcagc
     9061 tatccggcgt tttacctgtc ctcctttcaa cccgttgcgg tgctcaaggg actgcgtata
     9121 aaggtgaaca caggagccgg ctttatccgg aaagggctcg tcattaccca attcaccgtc
     9181 tccgtgactc ttatcgtttg cacggtcatc atctatcagc aggtgcaata tgcccgctcg
     9241 cgcgatctcg gttttgacaa agaacatctg atatctattt ctttacgcgg cgatcttgcc
     9301 caacacttcg acgccgtgcg cacggagttg atgcagaccg gcctcgtggc caacgcatcc
     9361 ctcagcgcca gcccccctct gtccatgtgg gagaccgtta catcccagga aattacctgg
     9421 gcgggagcgg acgcgggcaa aggtgtccgc atctgctggg agcacgtatc gccggaatat
     9481 ttttcgacca tgggactaca gatcaagaga ggccgcaact tttatcccga cgctaaagtc
     9541 gatagtaata acatgatcat caacggggcg atggcggaac tgatgggcaa ggaagggcgg
     9601 gtcggcggtt atctttccga tgggatcaga gctccgtaca ggatagtagg gatcgtaaaa
     9661 aatttcttat tcaatgatat gcatgcctct tccgtcgcgc cgctgatgct gagctgcggg
     9721 ccggtaagac cagggatgtt caatttcatg gaggtcaggc tcaaagccgg caatgacttg
     9781 cctaccgcat tgactaaggt ggagactatt ctgaaagcta acaatcccgg atatccggtc
     9841 gactaccggt ggacggacca ggatttcgaa ggctcgttct cggaggaggc gcacctcggc
     9901 cagttcgcag gtatcttctc gatgctggcc atcttcattt cctgtttggg cctgtttggc
     9961 ctggccgcct ataccgccga gcgccgcacc aaagagatcg gaatccgcaa gattctcggc
    10021 gcctccacca ccacactcgc cagtctgttg tccaaagagt ttttgcaact ggtcacgctg
    10081 tcctgtctga tcgccttccc gctcgcctgg tgggttatga atggctggct taaggattat
    10141 agttaccgca taaccatccg ttggtgggta ttcgcgttgt ccggcgccgc ggctgtgtcg
    10201 atcgccttgc ttaccgtcag cttcctggct atcaggaccg cccgcaccaa tccggttaaa
    10261 agtttacgta ccgattaacc ctcgcgccac cccccgatcc tcgcgccacc ccacaacccg
    10321 cacactatcg cccggtcctc gcgccacccg ccgacccgct cgccaccccc atcaatattt
    10381 gaacaccttt cctcccgcca tcacctcctg cgtcttttca tcgaagtacg ccttatgccc
    10441 cgtgtgtacc gcggcattgg tcatgatgtt tgcaatagaa tggctatacc ctgcctcaac
    10501 gggcgcattc ggcgttttcc ggctgcggat acactccatc cagttgcgga tatggttgga
    10561 ggtaagtttg tctccaccgg tatcggccga cgcgactacc ggttctgctt cggacaagct
    10621 catttccggc aacaggttcg gctgcatgtg catttgatcc gcaaaatgct ttgtcagacc
    10681 tccggtcggg gataccttat tggtgatcag gttgagttcc cctccattgg aatagtagat
    10741 ctcagcgggt ttttcttccc cattgtgcat gcgggaagcg aaagtcacct ggaatcccga
    10801 ggagggatca tcgtcgggac cgtaatcgaa aacggccgtg atggtatccc agttctttct
    10861 gccatccttc cagacataaa tgcccccgtt ggccacgacg ctgcgcggat gtttcaaacc
    10921 gctgaaccaa tgtaccgtat ctatctgatg gctcatccat tggcctggca gacccgatga
    10981 atagggccag aacaaccgga actcgagata tttcctggga tcgaaggtat catgaggcct
    11041 gttgagcagc cagcggttcc agtcggtatc ttcttctttc aattgcccta caagcgatgg
    11101 tctgcgccaa cgaccgggtt ggttgacatt ccaggtcagt tcgaccatcg tgatcggtcc
    11161 gaattttcca ctccggataa attcattggc cgcatgatag tttgcgccgc tccgtctttg
    11221 cgaacctatc tgaacgatcc tgtccgtcgc cttgattgcc ttcagggcat cccggttgtc
    11281 ttccatcgtc tcggcaaacg gtttttcgac ataagcgtcg caacctgctt ttaccgcctc
    11341 tatggcatgc cgcgcgtgct gaaaatcggc cgtgctcacg aatacggcat cgacaagttt
    11401 tttcgaatac aattcttcat tgttgcggca tgctacaata tcgtgctgca ttttgtcttt
    11461 ccatagcgcg gccccctgct ctcttctcat cttccagata tcggataccg ccaccacatc
    11521 gaaattcaat tctttgtaat gattcatgaa acagggaaca tgcgagctcc ggtgccggtc
    11581 ggaaaaccct acaacgccta cgcgcacccg atcattggcg ccgggtatcc tgcggtagct
    11641 gctggcgggg aagctcaggg cgcccatata cccggccacg ccggccatcg agctcttacg
    11701 gataaaatct cttctggatg ttgtcatatg gaagtgtcat ttagataaga taaatgtact
    11761 aatttaggtc attgctcacg caaacgactg aaatatgatc actctccgac tcctgatttt
    11821 acgcaaacgt tttcgttgga cttcgatgct atcgaccccg tcgaccccat cgatcccatc
    11881 gatcccacgt atcgcttcga tcgccattcc gatcctcctt gcactagccg gccttgcgct
    11941 ggtgccgggt acgctttccg cgcagcagtt gcctatcggt gttagcgtcg atgcggccga
    12001 caggcgtgtg gtcgtcaccg caggcggtca gcccttcacc acctttatat atcccgacag
    12061 cctggagaag cccgtgctgt accccatata tgctcccgac ggccagttga tcacacgcgg
    12121 ctttcccgtc agcccgcgcc cgggcgaacc cgtcgaccat ccccatcatc ttggtctttg
    12181 gttcaattat gaaaaggtga acggtctgga tttctggaac aactcctatg ctatcccggc
    12241 cgcaaaaaaa tcccagtacg ggtggataca caccgacagc attaatgggc attccgccgc
    12301 cagtcttcca ccgagcaaca gtgtgatgct cgggagaaat gaccttcgct ataaagcccg
    12361 ctggaccgat cagcaggggc atgtcctgct cgacgaggcg acacactttt tcttcagcgc
    12421 caccagagac gaacggatca tcgaccgcat caccaccctc acagcggtgt cggacggagg
    12481 agtgtccttt cccgacgcta aagacggcat gctgggtctg cgtgtcacga aggagcttca
    12541 gatacccagc aacgctcccg gccagttcgt ggacgacaaa ggaaatatta ccaaagtcgc
    12601 tgccggcaat acccccgaca tcaacggcaa ctatctcacc agcgagggta agcaggggga
    12661 cagcgcctgg ggcacccgcg gcacctggtg tctgctctac gggaaaaaga acaacgacat
    12721 gctgagtatc gcgatcatcg atcatcccgg caatcccggc tatcccacct actggcatgc
    12781 acgcgggtat ggcctttttg ccgccaatcc gctgggacaa tccatcttca gcaatggtcg
    12841 cgaaacgatg aacttcaggc tggcgaaagg acaatccgcc acattcaggt accggatagt
    12901 catagccgca ggcaaagacc ggctgtccac cggcaggatc aggcaactgg ccgatgcttt
    12961 tgcccatatc gaatgacatt accgggcaac caacctaaaa accgaaccat ggcgccccaa
    13021 aaatgttaat tttactgcgg ccggactaaa agaacggcac attaacttaa aatagtaaaa
    13081 tatgagcacc aggaacctct ccttaatcat tatcgcactg atcattttag tattgggagg
    13141 ctgcggatgc ggcggctata acaatatggt gagactcgac gagaacgtaa aaagcagctg
    13201 gggcaaggta cagagcgact atcaacgccg caatgacctg atccccaatc tggtcaatac
    13261 cgtcaagggg gcatccaatt tcgaacagca gaccctgacc aatgttatcg aagcaagggc
    13321 gaaagcgact tcggtccaga tcgatgccaa caacctgacc cccgaaaaga tcgcccagtt
    13381 ccagcaggcc cagggccagg tgagcagctc gctgagccgg ttgctggcgg tggtggagaa
    13441 ctatccagac ctgaaggcga cccagaacta ccgcgatctg caggcccagc tggaaggtac
    13501 ggagaacagg atcaaggtat cgcgcaacga cttcaacgac gccgtacagc agtacaattc
    13561 gaccgtccgg cgtttcccca ataatatatt cgcgggaatg ttcgggttcg cgaccaaggg
    13621 ttatttccag tccgagcccg gctcggagaa ggcgcccgaa gtcaagttct gatcctggtg
    13681 ccgccaacac cgatcgcggc gcccggaaaa gccgatcgct gctccgccaa caccgatcgc
    13741 ggcgcccccg gaaaagccga tcgctgcgcc gcccaaaccg atcccatcca ccgtttaaaa
    13801 ccattgtttt gtcattatcc ctcttcaaac gcaaaccgct gttcaacgaa gaccagcaac
    13861 aagagatcac cacggccatc cgggacgcgg aaaaatgcac cagcggcgag atccgcgtat
    13921 tcatcgaaag gcattgccgg tatatggatc cgctggacag ggccctcgag gtcttcgccg
    13981 cgctccagat ggaacgaacc gcggcccgca acggcgtgct ggtctatgta gcgctgaagg
    14041 accgtcagct ggcggttttc ggggatagag gcattcatga aaaagtgggg gtcgtcttct
    14101 ggaaccagca ggtgaagacg atcctctctc acttcaataa cgccgattat tcgcatggta
    14161 tcgcggcggt ggtcaccgag atcggaaaag cgctgcaaca acattttccg tacgatccgg
    14221 atacggacaa gaacgagctg ccggacgata ttgtcttcgg ccggtgatcc ggcgaataat
    14281 taagggcggc ccggtgtata atttccggca gcccggcgaa taaatcaacg caatgaagaa
    14341 gcttctcggg atactcatcc tcgccccttg cctgttggcc gtcaatttaa cccgcgccca
    14401 ggacgtaccg cccagaccga acccggctac cgcggtcaac gacttcgcgc atgtcatgac
    14461 ccctgaccag gtgtcggcgc tggaacagaa actgatcgcc tacaatgaca gcactacctc
    14521 gcagatcgtg atcgtcaccg tccccgatat cggggatgcg gccatcgagg actatgcgtt
    14581 gaaaatattg cgcagctggg gcgtgggaga taaaaagaac aacaatggcg tcgtcatcct
    14641 cgcggacatc aaggatcaca aggtgtatat cgccgtggga tatggactcg aaggagccat
    14701 tcccgacgcg acggccaagg agatcgtcga taacgagatc gtcccgaact tcagggccgg
    14761 cggcgatgac aattattacc ggggtttcga tcaggccgcc gacgcgctga tcaaagcggc
    14821 tgccggggaa tataaggcgc ctgcaggata cggacaacgc ggacggcgtc acggaggcag
    14881 cggcctggga gtcttcatca tcatctttat tgtcatcatc attatcatca gccgcagggg
    14941 cggcggtggc aggggaggcg gtggttatta tggcggcgga ggctggttac cctttatctt
    15001 aggcacgatg gtcggtcgcg gtctcggcgg cggaggagga ggatggggaa gtggcggagg
    15061 aggcggctgg agtggcggcg atggcggctt cggcggtgga ttcggtggag gcagcggcgg
    15121 cggcggtggt gccggcggca gctggtaaaa aggcattaaa aaacccccgg gtgatcgggg
    15181 gttttgatta tgcgcctccg ggtatcccgg tgaagccctc cggcgctaac cgctaatgat
    15241 gcgccttcgg ccaaatggcc tgtggccctt tatgttaaaa tcccttcttt tcgttagata
    15301 atcgagaagt gatataatcc gcctggtcct ggcttcccgc cgcctgtctt tttttggcga
    15361 caccctgaag catgttgtcg aagacggggt ccgtctgccc gcggtaagcc gacgggatgg
    15421 cctccttgaa ttcgatcaat agatctacac catgtttcag tttttcggta ttggagacct
    15481 tgcccagcat attggcgtag gccatcgaga aattgaattt agcctgtcct accggcatct
    15541 tttcgaaatt ttcggatatc gcatcgaaat tgctttcatc gccggatttg atgagcaccc
    15601 cggcaattgc taccgtgaga cctcctttgg cgggttcttt aaccaatttt ttggctacgt
    15661 ccagcgcctg ggtgctgtct atcgccgcca aagccgtgag cgcatttccg gcgacactat
    15721 aggatgagtc ggtagtagcc tttaggaaca aagccttgta ggaagcattt ttgtaagttc
    15781 ccagcaactc gatagccttg gccttcacgg ggtggttggg atcgtgttca gctatctcga
    15841 gaatgagggg ctcgaaggca gacctgacgg cgtcatgcga cagatcgatc ctggtcagcg
    15901 caaggttgcg cagccggtaa tagcggtccc gcatcgcggt tttcagcaga tcgatcgcct
    15961 tgggatcgtc ctggtgtttg gcgcaatatt ctatagcctc gcgacggtcg acatagttac
    16021 ctgcatattt gtattggaag acaaaattgg ccagcgtctt gttgtccttt ttttcgcaga
    16081 gcagcacctt gtccccgtcc acgttcacca gatcgggatg gctcttgtaa gcaaaggtga
    16141 aggtatccgt cttgtcctcg atccatacct tatgtctttc tttcgcaggc ccgttgtaga
    16201 cgtcgatcgc tatcggcaga cggaagacct tgcccgtctc ctgcgtttgg ttgacgatga
    16261 cctgaacctt gccggccgcg tcgtcataga catagtcgat cgccagcttg ggatgaccgc
    16321 tgccataata ccattggtcc caataccagt gcaggtcttc gcctgtcacg gcctcgaatg
    16381 ccagccggag atcggccgcc tccgcattcc cgaatttatg atcggtcagg tagaggtgca
    16441 aagccttgaa aaaagcgctg tcgccgacat agttccggag catgttgaga atgcgtcctc
    16501 cctttggata gctcacgccg tcgaaaacgt cttccttgtc ggcataatag tagcgaacga
    16561 gatctttctt cgcgttgtcg ggttgctgaa gatagctttg gatgcgggaa aaattgacgg
    16621 cctcacccgc atccttgccg tatttgtatt cgttccagag cgtctcgcta tagtcggcaa
    16681 aggactcgtt cagcgtgatg ttgctccagc tctcgcacgt caccaggtcg ccgaaccaat
    16741 ggtggaacag ctcgtgcgcg atcacgtcct cccactgatt gccgtccgtc agctcgcggg
    16801 catcctgttg tgccgcatcg ctgtgcagcg tcgcggtcgc attttccatg gctccgctga
    16861 catagtcgcg tcccgtgatc tggtcgtatt tcggccaggg atagtcgacg ccggtgatcc
    16921 gcgaatagaa ggcgatcatc tccggcgtat gcccgaagat ccggcgtgcc accggtgcat
    16981 attctttctc gacatagtag gctacttctt tgcctttgta agaatctttg acgatggcat
    17041 agtcgccgac acccatgaag aagagatagg gagagatggg ctcgtccagc ttccagtggt
    17101 cggtgcgggt gccgtcgccg ttggtgttct ggtcgacgag aagaccgttg gacagcgtga
    17161 catatttagc gggaactgtc atgtagatct cctcggtcgt tttctcgttg ggtttatcga
    17221 tcgtggggaa ccagacggaa ttggcttccg tctcgccctg tgtccatatt tccgtcggct
    17281 tggtcttgtc ctcacctttg gggttgatga aatagaggcc acgggcatct gtgatcgccg
    17341 cgctgccctg gtggactttc agctcgttgg gcttcgccgt ataatcgatg aaaagagtat
    17401 atttctccga acgcgtatag gtcttgtcga gatggatggt gacctgcatc ccgtcgtagt
    17461 catattttag cggggttttt ttcccggccc ggtcgatggc gatggaatgg atgtccatcc
    17521 ctttggcgtc cagggtgacg gtatcggtag
//
LOCUS       DASYSV010000194         6522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1315477, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000194 DASYSV010000000
VERSION     DASYSV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315477"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VGQ51_05970"
     CDS             <1..1225
                     /locus_tag="VGQ51_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEV9036143.1"
                     /translation="KNVGKYIEAQRLSERVNYDVEMIRELGYCNGVENYSRFFDRRMP
                     GTRPFCLLDYFPKDFLLVLDESHQTVPQISGMYGGDRSRKLVLVDYGFRLPSALDNRP
                     LNFHEFESMTGQTIYVSATPGDYELEKTEGVVVEQVVRPTGLLDPPIDIRPSAGQIDD
                     LLDEIDKRVKKGDRVLVTTLTKRMAEEMDRYLHRIDIRSKYIHSEVDTLERVEILRQL
                     RLGDIDVLVGVNLLREGLDLPEVSLVAILDADKEGFLRNEKSLTQTAGRAARNVDGLV
                     IFYADTMTESMQRTIDETNRRREKQIAYNEKHGIIPRTIIKSKEQVFAQTSVLDVKGY
                     NPGDPYAVAPDGELVSVVEEEQEVYQTIPQVEKAIARTKKEMEKAARDLDFMEAARLR
                     DEMFGLQKKLKEMKE"
     gene            1286..4213
                     /gene="polA"
                     /locus_tag="VGQ51_05975"
     CDS             1286..4213
                     /gene="polA"
                     /locus_tag="VGQ51_05975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV9036144.1"
                     /translation="MENTDKRLFLLDAMALIFRAYYALISSPRITSRGRNTNAQFGFT
                     NALVDLINNQHPSHMAVCFDTEAPTERHTDFSDYKANRQEAPEDLISALPDIKAIVQG
                     FNIPTVELDGFEADDVIGTLSKQAAAVGYEVYMVTPDKDYGQLVSEKIRIYKPGYQGG
                     LAEILGPKEVCEKWNIRDVAQVIDILGLMGDAVDNIPGIKGVGEKTAAKLLAEFGTLE
                     NVIAGADGIKGALGEKVRGGKESAILSKKLATIITDVPVEFHEENFRLKDWNKDALNE
                     IFNGLEFKTLGKRILGDGYATAGYVAPEAVQTDLFGNAVKPVATGKKKAAAADRLEEA
                     STRLEAAVEKGVDAAEGEAAGETSEPSEGAHPHMGKCISNTPHTYTAVVGDAAIKELV
                     EKLKTQKEICFDTETTGIDANDVDIVGMSFSITPGEAWYVPCCPPTVPQAQNIIDAFR
                     PLFESPDTVWIGQNLKYDLLVLKWYGVTLKGFVFDTMLAHYVIEPEGKRGMDQLSAQY
                     LGYEPVHIEELIGKKGKGQGNMRDVELEKIKDYAAEDADITLQLKHIFLPQLKEKAVE
                     KVFHEVENPLVKVLTDMEFEGIRVDTGFLLEYSKELDHEARQAEEKVYQQAGERFNLA
                     SPKQLGEVLFERMKLDPKAKKTKTGQYATGEDVLLKLAVKHQIVDDILAFRELTKLKS
                     TYVDALPLMINRKTGRVHTSYAQAVAVTGRLSSNNPNLQNIPIRTERGREIRKAFIPR
                     DEDYVLLSADYSQIELRIVAAISEDPNMCAAFREGKDIHTATAARVFGVDEKSVTKEM
                     RYKAKSVNFGIIYGQGAFGLADNLGISRTEAKEIIDNYKSQFPGIQKYMDSTINFARE
                     HGYVETLMGRKRWLRDINSANFTIRGFAERNAINSPIQGSAADMIKLAMIRIHEEFTR
                     QGFRSKMLLQVHDELVFDAYRAELDIIKPIIMRCMETALPLPQEVPALAEMGSGINWL
                     EAH"
     gene            4316..>6522
                     /locus_tag="VGQ51_05980"
     CDS             4316..>6522
                     /locus_tag="VGQ51_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036145.1"
                     /translation="MKSIRSIFSMLLAVILMAGAASATAAPAPQQQDGPPPQQPQDWP
                     RSFTTDDGTIVKIYQPQPEAFADNVLKSRWAISVLEQGKTDPVFGTFWSVANVETDRD
                     ARQVVIQSARVPNVRFPGQSDDNFTNSIKTALETNLPQVVGTLSLDELLAGLDQNTEQ
                     KKLSKDLNTQAPRIIYADQPSLMVLIDGQPKLQTNKDWGLDVVVNSPFTIVKGPDGNF
                     YLYGGKHWYQAQSATGPYSPAGNIPSQLQQVQSAVDNANNGNAGYVDSASAAQDRQIS
                     NIIVSTSPAELIQTDGRPNFTPISGTSLSFASNSSNDIFQDQSGQYYVLISGRWYASG
                     SLTGGWHYIASNSLPPDFAKIPNGSPKDNVLASVAGTDAAREAVMDAQIPQTAKVDRN
                     NASTSVNYNGDPQFAPLQGTDMQYATNTGSSVMEDNGMYYTVDNGVWFQSSSPNGPWV
                     VATARPGDVDRIPPTSPLYNTKYVYIYDVTPDYVYMGYTPGYLNTYIYGPTVVYGTGF
                     YYNPWYGGYYYPRPWTWGFNVCYNPWAGWTLGWGYGFGWFHYGLGFHGGWGGGWAGGG
                     WWGPHVYHPAYAWSRYRSYGFYGSNYYRNRNVYSTNFHTNIYAGRGGVVTRDNHTFYN
                     RTAIGRPSMNGAGRGFAPQGARPGGYSRPSGNGFNRSPRNNVFSDRQGNVYQRNATTN
                     QWQQRQSNSWRPLGNNPGVQQNLNRQQQMRDRGQVRSQNFQQSRSFSRPSGGY"
BASE COUNT         1662 a         1783 c         1782 g         1295 t
ORIGIN      
        1 caagaatgtc ggcaaataca tcgaagcgca gcggttgagc gaaagagtga actacgatgt
       61 ggagatgatc cgcgagctgg gttactgtaa cggtgtggag aactattcgc gttttttcga
      121 ccggcgcatg cccgggaccc gtcccttctg tctattggac tattttccga aagattttct
      181 cctggtgctg gatgagagcc atcagaccgt ccctcagatc agcggcatgt acggtggaga
      241 cagaagcaga aaactggtgc tggtggacta cggttttcgc ctccccagcg cactcgataa
      301 ccggccgctg aatttccatg aattcgaaag catgacgggg cagacgatct acgtatcggc
      361 gacgccgggg gactatgagc ttgaaaaaac ggaaggagtg gtcgtcgaac aggtggtccg
      421 tccgaccggg ttgctcgatc cgcccatcga cataaggccc agtgcaggcc agatcgacga
      481 tcttctcgac gagatcgaca aacgcgtcaa aaaaggagac cgcgtgctcg tgaccacgct
      541 gaccaaacgt atggccgaag agatggatcg ctatctgcat aggatcgaca tccgctccaa
      601 atatatccat agcgaagtcg ataccctcga acgcgtggag atcctgcgtc agttgcggct
      661 gggtgatatc gatgtcctgg taggtgtaaa cctcctccgc gaagggctcg atctcccgga
      721 agtatcgctc gtggctatcc tcgatgccga taaggaagga ttccttcgca atgagaaatc
      781 gctgacccag actgcgggtc gcgccgcgcg taatgtggat gggctcgtta tcttctacgc
      841 ggatacgatg accgaaagta tgcagcggac catcgatgag accaaccgcc gccgcgaaaa
      901 acagatcgcc tataatgaaa agcatggtat cattccccgt accatcatca agtcgaaaga
      961 acaggtcttc gcccagactt cggttctgga cgtcaaaggt tataacccgg gtgatcctta
     1021 tgccgttgct cccgatggcg agcttgtctc agtcgtcgaa gaggaacagg aggtttatca
     1081 gactatcccg caggtggaga aggcgatcgc acggaccaaa aaggagatgg agaaggcggc
     1141 ccgcgacctc gattttatgg aggcagcgag gttgcgggat gagatgttcg gactgcagaa
     1201 gaaattgaag gaaatgaagg aatagcgccg accctgcggg tcgccgtccc ctattaatcc
     1261 cggctaattg cctaaattcg cttccatgga aaataccgat aaaagattat tccttctcga
     1321 tgccatggcg ctcatcttcc gcgcctacta tgccctcatc agcagcccgc gcatcacgtc
     1381 caggggccgc aacaccaatg cgcagttcgg gtttaccaat gcccttgtcg atctgatcaa
     1441 caatcagcac ccttcacata tggcggtctg tttcgatacg gaggcgccga cagaaagaca
     1501 taccgatttt tcagattata aggccaatcg ccaggaggcg ccggaagacc tcataagcgc
     1561 tctgcccgat atcaaggcga tcgtccaggg tttcaatatc ccgaccgtcg agctcgacgg
     1621 atttgaagcc gatgacgtca tcggcacact gagcaaacaa gccgccgccg tgggatatga
     1681 ggtctatatg gtcacccccg acaaagacta tggacaactg gtctccgaaa agatccggat
     1741 ctacaaaccg ggatatcagg ggggacttgc cgaaatattg ggtccgaaag aggtttgcga
     1801 aaaatggaat atcagggacg tggcccaggt gatcgatatc ctgggtctca tgggagatgc
     1861 ggtcgataat atacccggca tcaaaggcgt cggggaaaaa acggccgcca agctgctggc
     1921 agaattcggc acactcgaaa atgtcatcgc cggagcggac ggcatcaaag gcgcgctcgg
     1981 agaaaaggtc cgcggaggaa aagagtccgc gatcctgagc aaaaagctgg ctacgatcat
     2041 cacggacgtg cccgtggaat tccatgagga gaatttccgg ctaaaggact ggaataagga
     2101 tgccttgaat gagatcttca acggtctcga attcaagacg cttggcaaac gcatcctcgg
     2161 ggatggatat gcaacggcag gatatgtggc gccggaagct gttcagaccg atctgttcgg
     2221 caatgcggta aaaccggtgg ctaccggtaa gaagaaggct gcggcggccg accgcctcga
     2281 agaggcaagc acccgtctcg aagccgcagt cgaaaaggga gtagacgccg ccgaaggaga
     2341 agccgccggc gaaacctcgg agccttccga aggggcacac ccgcacatgg gcaaatgcat
     2401 cagcaacacg ccgcatacgt ataccgccgt tgtgggcgac gccgctatta aagagcttgt
     2461 cgaaaaacta aaaacccaaa aggaaatttg tttcgacacc gaaacgaccg gcatcgacgc
     2521 caatgacgtc gacatcgtcg gcatgagctt ctccatcaca cccggtgaag cctggtacgt
     2581 accgtgctgc ccgcctaccg tgccgcaagc gcaaaatatc atcgacgcct tccgccccct
     2641 gttcgaatcc ccggataccg tctggatcgg acagaacctg aaatacgatc tcctcgtgct
     2701 gaaatggtac ggtgtaacgc tgaagggctt cgtcttcgat accatgcttg cccactacgt
     2761 catcgagccc gaaggcaaac gggggatgga ccagctgagc gcccaatacc tgggctacga
     2821 accggtgcat atcgaagagc tcatcgggaa aaaaggaaaa gggcagggca atatgcgcga
     2881 cgtggagctc gaaaagataa aagactatgc cgcggaagac gccgacatca cgctgcagct
     2941 gaaacatatc ttcctgccgc agctgaaaga aaaggcggtc gaaaaggtat tccatgaggt
     3001 cgagaaccca ttggtaaaag tgctgaccga tatggagttc gaggggatca gggtggacac
     3061 cggcttcctg ctggaatatt ccaaagagct ggaccacgaa gcgcggcagg cagaggaaaa
     3121 ggtctatcag caggcgggcg agcgcttcaa cctggcctca cccaaacagc tgggggaggt
     3181 acttttcgaa cgaatgaagc tggaccccaa agccaaaaag acaaagacgg ggcagtacgc
     3241 caccggtgag gacgtgttgt tgaagctggc cgtcaaacac cagatcgtgg acgatatcct
     3301 tgcctttcgc gagctcacca agctgaagtc gacttatgtc gatgcattgc cgttgatgat
     3361 caaccgcaag acgggacgcg tacataccag ctatgcccag gccgtcgcgg tcaccggccg
     3421 cctcagttcg aacaacccca acctgcaaaa tatacccatc cgcacggaaa gaggaaggga
     3481 gatccgcaag gcattcattc cgcgcgacga agattatgtc ctgctatcgg ccgattactc
     3541 tcagatagaa ttgcgtatcg tcgcggccat cagcgaagac ccgaatatgt gtgccgcctt
     3601 ccgcgaagga aaggatatcc atacggcaac ggccgccagg gtgttcggcg tggacgaaaa
     3661 atcggtcacg aaggaaatgc ggtacaaggc caaaagcgtt aatttcggca tcatctacgg
     3721 acagggcgcc ttcggtctcg cggataatct cggcatcagc cgtacagagg ccaaagagat
     3781 catcgacaat tataaatccc agttcccggg tatccagaaa tatatggaca gcaccatcaa
     3841 ttttgcacgc gagcatggct acgtggagac cctgatggga aggaaaagat ggctgaggga
     3901 tatcaattcc gccaatttta ccatacgggg ctttgccgaa cgcaatgcca tcaattcccc
     3961 gatccagggt tcggcggcag atatgatcaa gctcgccatg atccggatcc atgaggaatt
     4021 cacccgccag ggtttcagat cgaagatgct cctgcaggtg catgacgaac tggtcttcga
     4081 cgcttatagg gcagagctcg atataattaa acccataata atgcgttgta tggaaacagc
     4141 gctacccctg ccgcaggaag tgccagccct tgccgaaatg ggctccggga tcaactggct
     4201 cgaggcacac tagccggcgg tgatcattaa tagcttttta acatctttat ttaagaaccc
     4261 accaaacttt gcgcttctgt gatagtcaaa ggactactaa atgaaaaaca aacatatgaa
     4321 aagcattcgt agtatattct ctatgttgct ggcagtcatc ctgatggccg gcgcggcatc
     4381 cgcaacagcc gcacccgcgc cccagcaaca ggacggtccc ccgccccagc aaccgcagga
     4441 ttggccccga agcttcacca ccgatgatgg tacgatcgta aagatctatc aacctcagcc
     4501 tgaggctttt gcagataatg tgttgaaatc ccgctgggcg atttccgtat tggagcaggg
     4561 caaaactgat cccgttttcg gtaccttttg gtcggtagcc aacgtggaaa ccgacaggga
     4621 tgcacgccag gtcgtcattc agtccgccag agtgcccaat gtgcggttcc cggggcagtc
     4681 cgatgacaac tttaccaata gcataaagac cgccctcgaa acgaatcttc cacaggtggt
     4741 cggcacgctt tcactcgacg agctgctggc aggcctcgac caaaataccg aacaaaagaa
     4801 actctccaag gacctcaata cgcaggcgcc ccggatcatc tatgccgatc agccttcgct
     4861 gatggtcctc atcgacggac agcctaagct gcaaacgaat aaagactggg ggctcgacgt
     4921 cgtcgtcaac tccccgttca ccatcgtcaa aggccccgac ggtaatttct acctctatgg
     4981 cggaaaacat tggtatcagg ctcagtccgc taccgggccc tactcccccg ccggaaacat
     5041 cccttcccag ctgcaacagg tgcaatccgc cgtcgacaat gccaacaacg gaaatgcagg
     5101 gtacgtggac agcgcaagcg cagcgcagga tcgtcagatc tccaatataa tcgtcagtac
     5161 ttcccctgcc gaattgatcc agaccgacgg cagaccgaat tttactccga tctccggtac
     5221 cagcctcagc tttgcaagca actcctccaa cgatattttc caggatcagt ccggtcagta
     5281 ctatgtattg atctctggtc gctggtatgc atcgggatcg ctgacgggcg gatggcatta
     5341 tatcgccagc aactcgctgc ctcccgattt tgcaaagatc cccaatggca gtccgaaaga
     5401 caatgttctt gccagtgtag ccggtaccga tgccgctcgt gaagccgtca tggatgcgca
     5461 gataccgcag acggcgaaag tcgatcgtaa caacgcttct acctcggtga actataatgg
     5521 cgatccgcag tttgcgccgc tgcagggtac ggacatgcaa tatgccacga atacgggttc
     5581 ttcggtaatg gaagacaacg ggatgtatta taccgtcgat aatggcgtct ggttccagtc
     5641 gtccagtccg aacgggccct gggtggtggc cacggcacga cctggtgatg tggacagaat
     5701 accgccgact tctccgttgt ataacacgaa atatgtctat atctatgacg tgacgcctga
     5761 ttatgtatat atgggctata ccccgggtta cctcaatacc tatatctatg gaccgaccgt
     5821 cgtttacggt acgggcttct attataatcc ctggtatggc ggatactatt atccccggcc
     5881 ctggacatgg ggtttcaacg tttgttataa tccctgggcc ggatggacgc tgggatgggg
     5941 ttatggattt ggctggttcc attatggcct cgggttccac ggcggatggg gcggcggatg
     6001 ggccggcggc ggctggtggg gtccgcatgt ctatcatccc gcatatgcat ggagccgtta
     6061 caggtcctat ggtttctacg gatccaatta ttacaggaac cggaacgtct actcgaccaa
     6121 tttccacacg aacatctatg ccggccgcgg cggcgtagtc acccgcgata accatacgtt
     6181 ttacaaccgt accgcgatcg ggagaccgag catgaacggt gcgggcagag gctttgcccc
     6241 gcaaggcgcg agaccgggcg gatacagccg tccatcgggt aatgggttca acaggtcgcc
     6301 gcgcaataac gtattctccg accggcaggg caatgtttat caacgcaacg ccactacgaa
     6361 tcaatggcag caacgccaat ccaattcctg gcgcccgctg ggcaataatc ccggcgtaca
     6421 gcaaaacctg aaccgccagc agcaaatgcg cgaccgggga caggtacgtt cacagaattt
     6481 ccagcaatcc cgcagcttca gccggccgag cggcggctat ag
//
LOCUS       DASYSV010000195        11548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_549917, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000195 DASYSV010000000
VERSION     DASYSV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11548
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549917"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..533
                     /locus_tag="VGQ51_05985"
     CDS             306..533
                     /locus_tag="VGQ51_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036146.1"
                     /translation="MFAPHSIRLTSRGRPVRLLDRHFTEWLPVWVMVIEHPEGIFVID
                     TGECTREHPLVFPPSHDSEAGMRLSQLKIVP"
     gene            543..2792
                     /locus_tag="VGQ51_05990"
     CDS             543..2792
                     /locus_tag="VGQ51_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV9036147.1"
                     /translation="MRHTRRDFIRSAGCLTIGFCFGGEAFVSPASRALLQNADTSQGP
                     GLPGDLRENPRINAWLEVLSDGRVRVLTGKIELGQGIATAVAQVAAEELELDLSQVEV
                     LLAETGRTPDEGYTAGSNSIEASAMSVRYAAAAAKEKLLGLAAQKWNVPVTTLTTTKG
                     YIHLPGGARPASSHDDALSFSQVLEGRQITDEVRLPVTLKPRDQYRIVGTPALRQDNI
                     RIVGGQPAYVHDLRFPDMVHARIVRPPAYGATLSHLDESAVHKQFPNILKIVVNGSFL
                     GVIAAHEYEVIQAQRWLKQNAGWSEGPRLPKLAPGQLPSYLKTLPVKTERVGEKGDVA
                     SLQTNTTLKAEYFKPYLMHGSIGPSCAVAIFDNNKLDIWSHSQGVYPLRETLAALLHM
                     PVENIHVKGVPGSGCYGHNGADDAAADAALLALAFPGKHVRLQWSREDEHGWEPYGSA
                     MVMQLQAFLDDNGKISHWKYALWSDTHGTRPGGNPANILAARYIEHPLAARPSGFSGG
                     ANRNAEPYYKISNQLVEANFFNGPLRTSSLRSLGAYANIFAIESFMDELADKSGKDPF
                     EFRLLHLDDPRARDVLAKLQSVTADQTGTTSARQSQPGIGIAFSRYKNSGTYCAVAAR
                     VSVDTKTHDVRVQKMWAVVDAGEAINPDGIKNQIEGGLIQSASWTLYEQVRFDEKRIT
                     SLSWSTYPVMHFDQAPEVEVILIDRPAETPYGAGEAAQGPAAAAIVNAIFHACGKRIR
                     HLPVSETFA"
     gene            2925..3737
                     /locus_tag="VGQ51_05995"
     CDS             2925..3737
                     /locus_tag="VGQ51_05995"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEV9036148.1"
                     /translation="MAFNLLDSLKDIFTHDRVRAAAASLGETESNISKAISGIIPAII
                     GGIITRTDSGIDGASSILNMAKGASGTGILANLGNRFGDLQNSDFVTNGLDTAKRLLG
                     DKLDTVATAVSRYAGIRESSVFSLLAVTAPAALGVLGKYAADSNLTPAGLSNLLSAQR
                     PSVAASLPIGLSSLTGWIKGPAIPLRSAVPPTSDRVRHTSPPVPTPERTRHPSPPAPT
                     PEPRNFLPPRRKGFWPIFIGLTILLSLIFWIRGCNKNPTARNIGVRGFHNSV"
     gene            3761..4147
                     /locus_tag="VGQ51_06000"
     CDS             3761..4147
                     /locus_tag="VGQ51_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036149.1"
                     /translation="MNRKTFIFLSVAGAATLATPIAGCRHYSDTVLSTLATPDFLGHI
                     CDAKTIGNIGSAYRTAIPEESRKSDLIDILLKDIQGTPNAPRLAQLLKDRVGHDFATD
                     NVVTIGGWVLSRTEARQCAIYSFSQQ"
     gene            4156..5694
                     /locus_tag="VGQ51_06005"
     CDS             4156..5694
                     /locus_tag="VGQ51_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV9036150.1"
                     /translation="MHIDARQLPDNSLIEGDLCIIGTGPAGLSIALEWNNTPYKVILL
                     EGGGFDYEEKIQGLYAGQTTGQRYYPLKSTRLHYFGGTSNHWSGYCSTFDPIDFEKRD
                     WVPHSGWPIRRSDLDPFYERAQPILDLGPYEYDVAHWQKQDPGLKSLLPDNGAVWNKV
                     WQFSPPTRLNGKYSRTIKNSKNIHLYTYANLVDITANEAVTSIQSLTVKNLEGKQHTV
                     KARRFVLACNTIQNARILLACTKQAPKGLGNDRDLVGRFFMEHMQIKSAWLHLPAPDP
                     LRLYDIQPGITKMRCELSISREMQIKHRILNGTASFMPLSIARNARASIDSWNDQDPV
                     KSLKNFRNVSAAARRSAGTAPTTGTNTWMLSTRIEQAPNPDSRITLDTEKDAMGMPRA
                     ILHWVLTPLEKRSTRTIYELIGQQVGTAGVGRLQLMEHLQDPKDFSWPDFTSGGWHHM
                     GTTRMSDDPATGVVDAHNKMYGIDNLFIAGASVFATSAAPNPTLTITALSLRLSDHLR
                     SLYR"
     gene            complement(5670..5861)
                     /locus_tag="VGQ51_06010"
     CDS             complement(5670..5861)
                     /locus_tag="VGQ51_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036151.1"
                     /translation="MRHVCGEGRRDGVAEMGRRDGQWRWGGATGSGDGEARRGSGDGE
                     ARWGRGDASEAVYRYNDLR"
     gene            5883..7364
                     /locus_tag="VGQ51_06015"
     CDS             5883..7364
                     /locus_tag="VGQ51_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEV9036152.1"
                     /translation="MSRPSYWLTRWLILRLLGVVYAVAFLVAILQIIPLIGANGLTPV
                     GIYLQRIHDALGSTSAGFFRLPSIFWFTHSDTALLTVAWAGFLLSCIVIAGYANALML
                     VVLWLLYMSFVNAGQEWYGYGWEIQLLETGFLAIFLCPLLDPRPFPKRAPPFPIIVLF
                     RWLIFRIMLGSGLIKIRGDRIWRNSTALYYHFETQPMPGPLSRWFHFLPRGILRLGVW
                     YNWLAELIAPWFIFWPRLARHVAGIIIVLLQIILIFSGNLSFLNWLTIIPALACMDDG
                     FWAKLLPRRLAEKAIAADASAEPSPPMNITVWVVTAIVCLLSIQPAFNILSPNQVMNT
                     SYDPLNLVNTYGAFGSVGTERLNIVFEGTSDSIPDDKANWKEYPYKGLPVAPDKRPPQ
                     VAPYHLRFDWQMWFAAMSTPDEYPWTLTLISKLLHNDPTALSLFAGNPFPDKPPRYIR
                     AVLYRYKFALPGDPQHLWWTRQRVGDPWLPAMSANDPRLGAAQ"
     gene            7503..8075
                     /locus_tag="VGQ51_06020"
     CDS             7503..8075
                     /locus_tag="VGQ51_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036153.1"
                     /translation="MKHAAAILGVLVFLSACTSNPTPSTTDSSKIVSDSSLTRPIQSP
                     FDITYSSKFAMDEPTNAESVLKFWKAYDNGDFSTVKNLFADSFEVHLADGMSMHTSRD
                     STLADVTMFRNSLTASSSTVTAVMAVKSTDRDEHWVLIWGTEKDTHKDGKIDSVDLHE
                     AWQFNKNGQAMMLLQYKRSYAPPAAMKKKK"
     gene            complement(8167..9588)
                     /locus_tag="VGQ51_06025"
     CDS             complement(8167..9588)
                     /locus_tag="VGQ51_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase-like domain-containing
                     protein"
                     /protein_id="HEV9036154.1"
                     /translation="MKRLSLAGMLVLAICWYEPLAAQAVKNIRVIVLTDIEADPDDTQ
                     SLIRWLLYCNQWKVEGLIATTSIHQQNRVAPESILRVLDAYAKVQPNLLKHEKGYPTG
                     DELRAKVKKGLAVYGMQGVGEGKDSEGSDWIVRVLEQPDERPVWVTVWGGPNTLAQAL
                     WKIRKTVGAQAAEKIYRKLRVYTISDQDDSGPWIRQQWPSIFYIVTPGYNYTYATWLG
                     MSFPYPGANNEVTSNEWLARNIQQGHGPLGAAYPDVAYGMEGDTPSFLNLIDNGLNDP
                     EHPDYGGWGGRYALYLPEFRNSNTGMFRRENWPSDQPETRPIWTNAVDSVVSKTDRKS
                     YVGNQETIWRWRTEVQNDFAARMAWTTKAFSECNHPPVPVLAYGERLTVKSGQVFHLS
                     AAGTTDPDGDSMSYLWSQYPEAGSYKGIVSFRPYAANLYDLPVTAPQVDSVCTIHFIL
                     AVTDKGTPPLTRYRRVIVTVVPK"
     gene            complement(9590..10345)
                     /locus_tag="VGQ51_06030"
     CDS             complement(9590..10345)
                     /locus_tag="VGQ51_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV9036155.1"
                     /translation="MKIFKKDALEVRIYDDRIAMGSAAAEMVGQRINELLERQETVNI
                     VFAAAPSQNEFLAAVGEQPVDWHRVNAFHMDEYTGLPGDAPQSFGKFLREKLFSRVAL
                     RAVHYLDGNAANLAEECRRYRGLLEEHPTDIVVLGIGENTHLAFNDPHVARFDDPFWV
                     KAVKLDKVCRRQQVNDGSFATIDEVPEYALTMTIPALFRATYAYVIVPGERKARAMDC
                     TLNERITQRHPSTVLRRHPRAVLFADKESMPNC"
     gene            complement(10332..>11548)
                     /gene="nagA"
                     /locus_tag="VGQ51_06035"
     CDS             complement(10332..>11548)
                     /gene="nagA"
                     /locus_tag="VGQ51_06035"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV9036156.1"
                     /translation="KIYNGRVVTPFGVIGNGVVVMDGGKIVQVMEGRIAGTSLEIDAD
                     GALGIDADDALEIDAGGRWVVPGFIDIHVHGGGGHDFMDGTVEAFLGVAETHARYGTT
                     AMLPTTLTCSKETLFGLFGIYREAQRQNVKGAQFLGLHLEGPYLAMSQRGAQDPRYIR
                     NPDRGEYLEILERSGDIRRWSAAPELEGALGFGRELRSRGIVAALAHTDALYQEALAG
                     YEEGFTLATHFYSCMNGVTRRGVYRYAGAVEFAYLTEGMDVEIIADGVHLPEELLRLV
                     YRIKGPGRTALITDAMRAAGMPDGESILGSRRDGIRVIVEDGVAKLPDRSAFAGSVAT
                     ADRLVRVMIKAGIPLTDAVRMMSETPARIIGMEDRKGSLVPGMDADIVLFDDDIGIAM
                     TIIAGEVVYENI"
BASE COUNT         2547 a         3910 c         2593 g         2498 t
ORIGIN      
        1 gtcacggttt ggatgttgct atacccgaat tcgaattcca gcaccttccg catccctttc
       61 gagagtatga agtgatcgtc ggccaacaat atctcggtac tcatttcgcc ctgcaatttt
      121 cctacctacg cccggacgat attttctaac cagcgaagga ctttattttt gtcgactttt
      181 atctacaatt ccttgttcgg aaaactttat tttcgatttc aaataggata aggtttaatg
      241 gttatgggat ttgattagtc cgggcggctc ctccaacagg tgggagccgt ctctattttt
      301 gcactatgtt tgctccccac tccatccgac tcacctcccg cggccgtccc gtccgtctcc
      361 tcgatcgcca ttttaccgaa tggctgccgg tatgggtgat ggtcatcgaa catcccgaag
      421 ggatatttgt catcgacacc ggtgaatgca cccgcgaaca tccactggtc tttccgcctt
      481 cacacgacag cgaagcgggc atgcgtctct cacaactgaa gatagttccg taactttata
      541 gtatgcgtca cacgagaaga gacttcataa ggtcggccgg ttgtctcacc atcggattct
      601 gtttcggtgg cgaagccttc gtgagccccg cctcgcgcgc ccttctccaa aacgccgaca
      661 catcacaagg acccggtctg cccggcgatc ttcgcgaaaa cccccgtatc aatgcctggc
      721 tggaagtact gtcagacgga cgcgtacgcg tcctcaccgg caagatcgag ctcgggcagg
      781 gtatcgctac cgcagtagca caggtggccg ccgaagaact cgaactcgat ctgtcacagg
      841 tggaagtcct ccttgccgaa acaggccgca ctcccgatga aggctataca gccggcagca
      901 attccatcga agccagcgcc atgtcggtac gctatgccgc agccgcagcc aaagaaaaat
      961 tactcggact cgccgcacaa aaatggaatg tgcccgtcac cactctcacg acgacaaaag
     1021 gatatatcca cctcccgggc ggcgcccgcc cggcatcttc gcacgacgat gccctctcct
     1081 tctcgcaggt tttggaaggc cgccagatca ccgacgaggt ccgccttccg gttacactca
     1141 aaccccgcga tcaataccgc atcgtaggaa cacccgccct cagacaagac aacatccgca
     1201 tcgtcggagg tcagcctgca tatgtccacg accttcgctt ccccgatatg gtacacgccc
     1261 ggatcgttcg tcccccggcc tacggcgcaa cattgtcgca tctggacgaa tccgccgtcc
     1321 ataaacaatt cccgaacatt ttgaagatag tcgtcaacgg cagcttcctc ggcgtcatcg
     1381 ccgcccacga atacgaggtc atacaggcac agcgatggtt aaagcaaaat gccggatggt
     1441 ccgaaggacc acgccttcct aaactcgctc ccggccagtt gccatcttac ctcaagaccc
     1501 tcccggtgaa gaccgaacgg gttggcgaaa aaggcgacgt cgcttcactc caaacgaaca
     1561 ccacgctaaa agccgaatat ttcaaaccct acctgatgca tggctccatc ggtccttcct
     1621 gcgccgtagc catattcgac aacaataagc tggatatctg gtcgcatagc cagggggttt
     1681 atccgttacg cgagacgctg gccgcactcc tgcacatgcc cgttgaaaat atacatgtaa
     1741 aaggcgttcc cggatcgggc tgctatggtc acaacggcgc cgacgacgcc gcagccgatg
     1801 ccgcactcct ggcgctggcc ttccccggaa aacacgtccg tttgcaatgg tccagagaag
     1861 acgaacacgg ctgggagccc tatggcagcg caatggttat gcagctgcag gcttttctcg
     1921 acgacaacgg taagatatcc cattggaaat atgcgctatg gtccgatacc catggcacac
     1981 gaccaggagg taatcccgcc aatatccttg ccgcacgcta tatcgaacat cctttagcgg
     2041 cacgcccttc cggtttcagc ggcggcgcca accgcaacgc agaaccctat tacaagatct
     2101 ccaaccagct ggtggaagca aatttcttca atggcccgct acggacttcc tcactgcgca
     2161 gcctcggcgc ctatgccaac atctttgcca tcgagtcttt catggatgag ctggcagaca
     2221 aatccggcaa ggatcccttc gaattccgcc tccttcacct cgacgatccg cgggcgcgcg
     2281 acgtgctcgc caaattgcaa tcggtcaccg cagaccaaac cggcaccaca tccgcccgcc
     2341 agtcccaacc cggcatcggc atcgccttct cgcgctacaa aaattcaggc acctactgcg
     2401 ccgtggcagc acgcgtatcg gtcgacacca aaacccatga cgttcgcgtc caaaaaatgt
     2461 gggccgtcgt cgacgccggc gaagccatca atcccgacgg catcaaaaac caaatagaag
     2521 gcggcctgat acaatccgcc agctggaccc tctatgaaca agtgcgattc gatgaaaaaa
     2581 ggatcaccag cctcagctgg tccacctatc ccgtcatgca ttttgatcag gccccggaag
     2641 tagaggtgat cctgatcgac cgtcccgctg agacaccata cggtgccggt gaagccgctc
     2701 agggccccgc cgccgcagcc atcgtcaatg caatcttcca cgcttgcgga aaaaggatcc
     2761 ggcatctccc cgtctcggaa acctttgcat aattcacccg cctcgatttt tttatattct
     2821 gattaaatcc tattttgcct tacccacctt gccttaccca ccttgcctta cccaccttgc
     2881 caggccatcc atccgggatc gaaaagcata aattggttcg cgccatggca tttaacttat
     2941 tggattcact gaaagatatc ttcactcacg atcgggtccg cgcagcagcc gcctcgcttg
     3001 gcgaaaccga aagcaatatt tcaaaagcga tctcaggcat cattccggcc attatcggtg
     3061 gcatcatcac ccgcaccgat tcaggcatcg atggcgccag cagcatcctg aatatggcaa
     3121 aaggtgcatc gggcacaggt atactcgcca atctcggcaa ccggtttggc gatctgcaga
     3181 acagcgattt cgtcaccaat ggactcgaca ccgcaaaacg tctgctgggg gacaagctcg
     3241 atacggtcgc gaccgccgtc tcccgttatg ccggcatccg cgaatcctcg gtcttctctc
     3301 tcctcgccgt caccgcgccc gcagcccttg gcgtgctggg gaaatatgcg gccgattcca
     3361 atctgacacc tgccggcctt tccaatcttt tatccgctca acgaccctcc gtcgccgctt
     3421 cactgccgat aggtcttagc tcgctgaccg gctggatcaa gggccctgcg ataccgctca
     3481 gatcagcagt gccgccaacc tccgatcgcg tccgtcatac ctcacctccg gtgcccaccc
     3541 ccgaacgcac ccgacatcct tctccaccgg cgcccacccc cgaaccccgc aacttccttc
     3601 cgccccggcg aaaaggattc tggcccattt tcatcggcct gacgatcctg ctctcactca
     3661 tcttctggat cagaggctgt aacaaaaacc caaccgcccg gaacatcggg gttcgcggtt
     3721 tccacaattc tgtctaactt ggtgactaca gccaggccat atgaaccgaa aaaccttcat
     3781 tttcctttcc gttgccggag cagccactct tgctactcct atcgccggtt gccgtcatta
     3841 cagcgatact gtccttagca ccctcgcaac tccggatttc ctcggacata tctgcgacgc
     3901 gaagaccatc ggtaatattg gctcagccta ccgcaccgca attcccgaag agtcccgcaa
     3961 gagcgatctc atagatatcc tcctcaaaga catacaaggc accccgaacg ccccccgtct
     4021 cgctcaactg ctgaaagata gggtgggcca cgatttcgcc accgacaatg tcgtcaccat
     4081 cgggggctgg gtactctccc ggaccgaggc ccgccaatgc gcgatttact cctttagcca
     4141 gcaatagatt gactcatgca tatcgatgcc cgacaattac ccgacaattc cctgatagaa
     4201 ggcgatctct gcatcatcgg caccggcccc gcaggtctta gcatcgccct cgaatggaac
     4261 aataccccct ataaggtcat tctcctcgaa ggcggaggat tcgattatga agagaagata
     4321 cagggcctct acgcaggtca aaccaccgga cagagatatt atccgctgaa atccacgcgt
     4381 cttcactatt tcggcggcac cagcaatcac tggtccggct attgctccac tttcgatcct
     4441 atcgattttg aaaaaagaga ttgggtcccg catagcggct ggccgatacg gcgatccgac
     4501 ctcgacccct tctatgagag ggcgcaaccc atcctcgatc tgggacccta tgaatacgac
     4561 gtagcgcact ggcagaaaca ggaccccggt ctcaagtcgc tgctacccga caatggcgcc
     4621 gtctggaaca aagtttggca attcagtcca cccacaagac tcaatggcaa atattcccgt
     4681 acgatcaaaa actcgaagaa cattcatctt tatacgtacg ccaaccttgt agatatcacc
     4741 gccaatgaag ccgtgacgtc tatccaaagt ctcacggtca aaaacctcga aggtaaacag
     4801 cacaccgtaa aagcccgtcg cttcgtcctc gcatgcaata cgatccagaa tgcccgtatc
     4861 ctgctggctt gcaccaaaca ggcgccaaag ggtctcggca atgaccgcga cctcgtaggc
     4921 cgcttcttca tggaacatat gcagatcaaa tccgcatggc tccatctgcc cgcacccgat
     4981 ccgctcaggc tttatgatat ccagcccggc atcactaaaa tgagatgcga actttccatc
     5041 agccgtgaga tgcagatcaa acatcgcatc ctcaacggca ccgcctcgtt catgcccctc
     5101 tccatcgccc gcaacgccag ggcctccatc gacagctgga acgatcagga cccggtcaaa
     5161 agcctgaaaa atttcaggaa cgtatcggct gccgcacgtc gcagcgccgg cacggccccg
     5221 accaccggca ccaacacctg gatgctttcc acacgcatag agcaggcccc caaccccgat
     5281 tcccgcatca ccctcgatac cgaaaaggac gccatgggaa tgccccgcgc cattctccac
     5341 tgggtgctca ccccactgga aaaacgcagc acccggacga tctatgagct catcggccaa
     5401 caggtcggca cggcaggagt cggacggctt caactgatgg aacatctgca ggatccaaaa
     5461 gatttttcct ggccagattt taccagcggc ggctggcacc atatgggcac cacgcgaatg
     5521 agcgacgatc cggctaccgg cgtggtcgac gcccataaca aaatgtatgg tatcgacaat
     5581 ctttttattg ccggcgccag cgttttcgcc acctccgcgg cacccaatcc cactctcacc
     5641 atcacggccc tcagcctgcg gctgtcggat cacctgagat cgttgtaccg gtagactgcc
     5701 tcgctcgcat ctccgcgacc ccatcgcgcc tccccatccc cgctgccccg tcgcgcctcc
     5761 ccatctccgc tgcccgtcgc gcctccccat ctccactgcc cgtcgcgcct ccccatctcc
     5821 gcgaccccat cgcgcctccc ctccccacac acatggcgca aaaattgcta attttattac
     5881 gtatgtcccg cccctcctac tggctcactc gctggctcat ccttcgcttg ctgggtgtgg
     5941 tatacgccgt tgcctttctc gtcgcgatcc ttcagatcat accactcatc ggggccaacg
     6001 gtctgacacc ggtcggcatc tatctgcaac gtatacacga cgcgctcggc tccaccagcg
     6061 ccggcttttt ccgccttccc tcgatcttct ggttcaccca ctccgatacg gccctcctga
     6121 cagtggcgtg ggccggcttc ctcctctcct gtatcgtcat cgcgggttac gccaatgccc
     6181 tcatgctggt tgtcctgtgg ttgctgtata tgtccttcgt caatgccgga caggaatggt
     6241 acggctacgg ctgggagatc cagctactcg aaaccggctt cctcgccatt tttctctgcc
     6301 cccttttgga cccccgccct ttccccaaac gcgcgccacc ctttcccatc atcgtcctgt
     6361 ttcgctggct catcttccga atcatgctgg ggtccgggct gatcaagatc cgtggcgacc
     6421 ggatctggcg aaactcaacg gcgctttact accatttcga aacacaaccc atgcccggtc
     6481 cgctgagccg gtggttccat ttccttccgc gcggcatatt gcggttaggc gtctggtaca
     6541 actggctggc cgaactcatc gctccatggt tcatcttctg gccgcgtctc gcccgtcatg
     6601 tcgcaggcat catcatcgtt ctcctgcaga tcattctgat ctttagcggg aacctctcct
     6661 ttctcaattg gctgaccatc atcccggccc tcgcctgtat ggacgacgga ttctgggcaa
     6721 agctgcttcc ccgccgcctt gcggaaaaag caatagccgc ggatgcctcc gccgaaccgt
     6781 ccccaccgat gaatattact gtctgggtag tcaccgccat cgtttgctta ttgagcatac
     6841 agccggcttt caatatcctc tcccccaatc aggtcatgaa tacctcctac gatccgctca
     6901 atctcgtcaa tacttatggc gcattcggtt cggtgggcac cgaaagattg aatatcgtgt
     6961 tcgaaggcac ttccgattcg atacccgatg acaaagccaa ctggaaagaa tatccatata
     7021 aaggactgcc tgttgcgcct gacaaaagac ccccgcaagt ggccccctac catcttcgct
     7081 tcgactggca gatgtggttc gccgctatgt cgacccccga cgaatatccc tggaccctga
     7141 ccctgatctc caaactcctg cacaatgatc cgacggcgct cagcctgttc gccggtaatc
     7201 cctttcccga caagccgccc cgttatattc gcgcagtcct gtatcgctac aagttcgcgc
     7261 tgcccggtga tccacagcat ctctggtgga cccgccaacg cgtcggagac ccatggctcc
     7321 ccgccatgtc cgccaacgac ccccgcctgg gcgcggcaca atgatcattc gcccccgagc
     7381 gcaccacccc cgacactgcg taatgatttt cacccccgcc ccacccaata cgctcccatt
     7441 tgaatttttt tccatacatt aggtgtatcc ccttattttt ttacacctaa acatacacca
     7501 acatgaaaca tgccgcagcc atcttaggcg tcttagtctt cctgtcagcc tgtaccagca
     7561 accccacgcc gtcgaccacc gattccagta aaattgtctc cgattcttcg ctgaccagac
     7621 cgatccagtc cccttttgat atcacctact cctcgaaatt cgcaatggat gaacccacca
     7681 acgccgagtc ggtattgaaa ttctggaagg cgtatgacaa cggcgatttc agcacggtaa
     7741 agaacctatt cgccgactcc ttcgaggtcc atctggcgga cggcatgtcg atgcacacaa
     7801 gccgcgattc taccctggct gacgtaacga tgttcaggaa ttcgctgacg gcctcctcca
     7861 gcacggtcac cgctgtcatg gccgtcaaaa gcaccgaccg cgatgaacac tgggtgctca
     7921 tctggggtac cgaaaaagac acgcacaaag atggcaagat agactccgtc gatcttcatg
     7981 aagcctggca gttcaacaaa aatgggcagg ccatgatgct tctccagtac aagcgatcct
     8041 acgcaccccc tgccgctatg aagaaaaaga aataatcgcg tccccgcgca accaacccgc
     8101 gtcaccgcgc aaccaacccg ccctcacgcg acccgcgcga caaacccgcc ctccccggcg
     8161 ggttttttat ttcggcacca cggtaacgat cacccgccgg tacctcgtca acggcggcgt
     8221 ccccttatcc gtcaccgcca atatgaaatg gatcgtacaa acgctatcca cctgcggcgc
     8281 tgtcaccgga agatcataca ggttcgcagc ataaggcctg aagctcacga tacccttata
     8341 gcttcccgct tccggatatt gcgaccatag ataactcatc gaatccccat ccgggtccgt
     8401 ggttccagca gcgctcaaat ggaacacctg ccccgacttc accgtaagac gctcaccata
     8461 cgccaatacc ggcaccggag gatgattgca ctcgctgaaa gccttcgtgg tccatgccat
     8521 acgcgccgca aaatcattct gcacttccgt ccgccatcgc cagatcgtct cctggttacc
     8581 cacataactt ttcctgtctg tcttcgatac gacagaatcc acggcattgg tccagatagg
     8641 ccgggtctcc ggttgatcgg atggccaatt ctcgcgtcgg aacattcccg tattcgaatt
     8701 cctgaactcg ggcagataca acgcatacct ccctccccat ccgccatagt cgggatgttc
     8761 gggatcgttc agtccattgt cgatcaaatt cagaaaggaa ggtgtatcgc cttccatccc
     8821 atacgcgacg tcgggataag ccgcacccaa cggaccatgc ccctgctgaa tatttctcgc
     8881 cagccactca ttggaggtga cttcgttatt ggcccccggg taagggaacg acatacccaa
     8941 ccaggtcgca tatgtataat tatatcccgg cgtaacgata taaaaaatgg agggccactg
     9001 ctgacgtatc caggggccgc tgtcatcctg gtccgagatc gtatagacac gcagctttct
     9061 gtatatcttt tccgccgctt gcgcccctac ggtcttcctg atcttccata aggcctgcgc
     9121 caatgtgttc gggccgcccc aaacggttac ccataccggc cgctcatcgg gctgctccaa
     9181 taccctgacg atccaatccg atccctccga atccttgccc tccccgacgc cctgcatccc
     9241 ataaacggcc agaccctttt tcactttcgc ccggagctcg tcaccggtag gatacccttt
     9301 ttcatgtttt aagagattgg gctgcacttt cgcataggca tccagcacac gaaggatgct
     9361 ttccggggca accctgtttt gctgatgtat cgaggtcgtg gcgatcaacc cctctacctt
     9421 ccattggttg caatacaata gccatcggat cagcgattgg gtatcgtcgg ggtcggcctc
     9481 gatgtctgtc agcacgatca ccctgatgtt tttgacagct tgcgccgcca gcggctcata
     9541 ccagcatatc gccagcacca gcatcccggc aagagataat cttttcatat tagcagtttg
     9601 gcatactttc cttatctgca aacagcaccg cgcgcggatg tcttctcagg acggtcgaag
     9661 gatgcctctg ggtaatccgc tcattcagcg tacagtccat cgcccgggcc ttcctttccc
     9721 cgggcacgat cacataagca taggttgccc tgaacagcgc agggatcgtc atcgtcaatg
     9781 catactcagg cacctcgtcg atcgttgcaa aactcccgtc attcacctgc tgacgtctgc
     9841 aaaccttgtc cagcttcacg gccttcaccc aaaatggatc atcgaatctc gccacatgcg
     9901 ggtcattgaa ggcaagatgt gtattctctc cgataccaag taccacgata tcggtcggat
     9961 gctcctccaa taagccgcgg tatctgcggc actcctccgc gaggttcgca gcattcccat
    10021 ccaaataatg caccgcgcgt aaggccaccc tcgaaaataa cttttccctc aggaatttcc
    10081 cgaagctctg tggcgcatcg ccggggagcc cggtatattc atccatatga aaggcattga
    10141 cccgatgcca atcgacaggc tgctctccca ctgccgccag gaactcgttt tgcgatggcg
    10201 cagccgcaaa aacgatattg acagtctcct gccgctccaa caattcattg atccgctgac
    10261 cgaccatttc cgccgccgcc gatcccatgg cgatacggtc gtcgtatatc cttacttcga
    10321 gcgcatcctt tttaaatatt ttcataaacg acctcccctg cgataatggt catggcgatc
    10381 ccgatgtcgt catcgaaaag cacaatatcc gcatccatcc ccggaaccag tgaccccttc
    10441 cgatcctcca taccgatgat ccgcgccggc gtctccgaca tcatccgcac tgcatccgtc
    10501 aacgggatac ccgcttttat catgacacgt accagtctgt ctgcggtggc cacactaccc
    10561 gcgaaggcgc tgcgatcggg cagcttcgcc acaccatcct ccacgatgac ccgtatccca
    10621 tcccgtcggc ttccgagtat actttcccca tcgggcattc ccgcggcacg catcgcatcg
    10681 gtgatcagcg ccgtccgccc cggccccttg atccgatata caagcctcag caattcttcg
    10741 ggtaaatgca ctccgtccgc gatgatctcc acatccatgc cctccgtcag atacgcgaac
    10801 tccacggccc ccgcataccg gtatactccc cgccttgtga cgccgttcat gcaggaataa
    10861 aaatgcgtcg caagcgtaaa tccctcctca tatcccgcca gcgcctcctg gtacaacgca
    10921 tccgtatgcg ccagcgccgc tacgatgccc ctggacctca gctcccgtcc gaaacccaaa
    10981 gcaccctcca gttctggtgc ggcgctccat cgtctgatgt cgcccgacct ctccaatatc
    11041 tcaaggtatt ctccgcgatc cgggtttcgg atgtaccgcg gatcctgcgc cccacgctgg
    11101 ctcatcgcca gatagggtcc ctccagatgc aaccccagga attgcgcgcc cttcacattt
    11161 tgccgctgcg cttcgcgata tatcccgaac agaccgaaaa gcgtctcttt actacaggtg
    11221 agcgtggtgg ggagcatcgc cgtcgtccca taccgggcat gcgtttccgc gacacctaaa
    11281 aaagcttcca ccgtcccatc catgaaatca tgcccgccgc ccccatgcac atgaatatcg
    11341 ataaatcccg gtacgaccca ccgcccgccc gcatcgatct caagagcatc gtctgcatcg
    11401 atcccaagag cgccatccgc atcgatctca agcgaggtgc ccgcgatccg cccctccatc
    11461 acctgaacga tctttccccc atccatgacc accacaccgt tcccgatgac gccaaaaggt
    11521 gtcactaccc gtccattgta tatctttt
//
LOCUS       DASYSV010000196         7749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1958254, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000196 DASYSV010000000
VERSION     DASYSV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958254"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VGQ51_06040"
     CDS             <1..352
                     /locus_tag="VGQ51_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036157.1"
                     /translation="RQEGTRIVFTCKEPVDLLVGFFNRKSSAFLAAPQLETDASANDY
                     GQAEPRIANALAVAGMPPVNVHAWSFAPGEHTLTLGKGLCLVLGFAKPMGKISTYDAG
                     VDEPGSKNLDWLFE"
     gene            356..3130
                     /locus_tag="VGQ51_06045"
     CDS             356..3130
                     /locus_tag="VGQ51_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEV9036158.1"
                     /translation="MKRKIILFLLFIGHMAAAQDLRLWYRQPAKIWTEALPIGNGNLG
                     AMIYGGMANERLQLNESTFWTGQPRSYQREDAWQYLDTIRQLLFDGRQAEAETLAQAH
                     FMGKKFPDEKAYDPLKAAWFHKVRSDTSLMSADDSHWPTMKIPTPDGWEAAGLDGVDG
                     AVWFRTTFDVPAGWKGKDVVLDLGRIRDADFTYVNGVLVGHDEGTSKKRVYTISASML
                     KPYHNEIAIHVLNYNDKGGLTGRKGESNLFLRQDGQSIALPHDWKYAIQDGNPPPMPQ
                     YEAAYQPFGDLYLNFPVYQPGDYKRELDLRTAIAKTSYSDNGVHYTREYFASAPNRAI
                     VIHLTADRPGKISFQLAMKTSEHVMSNHRIDEHTFALMETAHNGVLHGVAFVHVETRG
                     GHNEGNMVSGADEATITLVGATSFVNYHDVSGDPERICRARINALRGLSWESLKTAHL
                     QEYRPLFNTFSLRLGKSDTTLPTDERILHYTPGSDPGFIALYVQYARYLLLSSSRPGA
                     SYPANLQGIWNDQLDPPWGSKFTTNINLEMNYWPAEPLHLGECSAPLFRLLQNLSQAG
                     KATAKDYYRAPGWVLHHNTDLWCGTAPINASNHGIWPTGGAWLCHQIWEHYLFTKDEK
                     FLQQYYPIMRSCATFFLSFLIKDPKTGWLISTPSASPEHGGLVAGPTMDHQIIRDLFK
                     NCIAAEAILRHYPEFMNELRDKLSRIAPDRIGRYGQLQEWLEDKDDTTDTHRHISHLW
                     GVYPGTDITWSDSAMMRAARQSLVYRGDEGTGWSLAWKVNCWARFRDGDHALRLVDKL
                     LSSAVGATGEHGGVYPNLFDSHPPFQIDGNFGGAAGIAEMLLQSQDSIVDLLPALPAV
                     LARGAVNGICARGGFELDMQWESGRLENVVVLSRAGGPCVLRYRGHVVRLSTTAGKKY
                     RLTGELKVLN"
     gene            3130..6051
                     /locus_tag="VGQ51_06050"
     CDS             3130..6051
                     /locus_tag="VGQ51_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEV9036159.1"
                     /translation="MRLLFFLLMPMAVLGQRRDILLDGGWTSALNGRHWVAVDIPHNW
                     DDYGGYRRLRHGNLHGYALYRRNLTVDKNSGLRYFLWFEGVGSYATVWVNGRKLGYHA
                     GGRTSFTVDITLALRPGHATNVIDVRADHPAFIRDLPWVCGGCSDERGFSEGSQPMGI
                     FRPVHLVITAPTRIEPFGVHVWNDTTISERNATLYLETEIHNYGTTPVEGLVIQKLFD
                     AHGAPVAITQTQQRIAPGQTVTVSQQLKDIPDPHLWSLNTPYLYSLKTSFGGDGVVTS
                     YGIRRIHWPDLHTPGPHPFLLNGRPVFINGIAGYEHRIGNSHAFTAEEIRARVQQIRA
                     AGFNAFRDAHQPHNLLYQHYWDSLGMLWWPQLSAHIWFDTPAFRQNFKTALTEWVKER
                     RNSPSVILWGLQNESKLPEDFARECVALIRQLDPTASSQRLIVTCNGGTGTDWDVPQN
                     WTGTYGGDPATYAADLRRQVLVGEYGAWRTIDLHGDVPNSEDRMCRLMETKIRLADSV
                     KGEVAGHFAWIFSSHDNPGRVQSGEGLREIDRIGPVNYKGLLTSWEEPVDAYYLYRSN
                     FVDAARSPMAYIVRGVSSPIVVYSNCDSVELFNDVDSISLGKRTRGGIGTHFEWDSAD
                     IRYNVLYAVGWYHGRPVVHDCTVLDHLPRSPHFRRLVGGAELLAAAPGYHYLYRVNCG
                     GPAYTDGAGQAWMADRHYTGGKTWGSLSWTDDYKGLPPFFASQRWTADPIAGTGDWPL
                     FQNFRYGLGKLAYTFPVQDGDYRLELYFAGPWLGAGGGMDCTGWRVFNVAVNGKTVLH
                     DLDIWREAGRNRALKKVIRVHVTGGKLVLSFPHVAAGQAIIDALAIATTDPGMTPAPP
                     SAPDTAKIRLEPAYDLRPVTSYKGDSTKWDIEVGVGDKYSLTVKYRWLKTPVTARLEL
                     RMEDGTLLHSETVTLQTTPPAKWNYLNTTTGTMINAGRYTIRLIVPSREGLRVDELQV
                     Q"
     gene            6059..>7749
                     /locus_tag="VGQ51_06055"
     CDS             6059..>7749
                     /locus_tag="VGQ51_06055"
                     /inference="COORDINATES: protein motif:HMM:NF020120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEV9036160.1"
                     /translation="MKRFVAGIMLLIASSAMGQEIKLTNLRADARGNDDRRPWLSWEL
                     RSSHHDVLQSAYRIMLFDDAGAVWDSHKQESAVSTGVVYDGAALASGHTYYWKAMVWD
                     RGRNDSSKWSTVDTFQTGLFTPADWKGAGWIGYEALPDSALRLPGTEKKKGPYRDTLP
                     LFRKEFRVGRPLHRATAFISGLGQFELRINGEKTGDHFLDPGWVDFAKKALYVALDIT
                     SRLHQGSNALGVMLGNGFYYIPRERYHKLLTAYGYPKMIARILLEYADGSREDIVTDS
                     SWRTAPGPVTYSSIYGGEDYDARRERIGWDRPGFDDKAWRVPVIVNGPPALEEQTADP
                     LKVFEHFGPRDIARRSPSSWVYDLGQNASGIIRLRVSGPAGAVMRITPAELIRGDSVN
                     QTATGKPYYWQYILKGQGIETWQPRFTYYGFRYLQLDGAVPEGQPNPGHLPVVSVLEG
                     LHTRNAMERTGRFTCSNLLFRRIDSLIDWAIQSNAASIFTDCPHREKLGWLEEAHLMG
                     NSLRYSYDIAPLYRKIVRDMMDAQTPDGLIPEIAPEYTVFDNPFRDSPEWGSSSIL"
BASE COUNT         1664 a         2134 c         2286 g         1665 t
ORIGIN      
        1 gcgacaggaa ggcacccgca tcgtctttac ttgtaaggaa cccgtagatt tgctggtcgg
       61 ctttttcaac cggaagtctt ccgcctttct ggcggcgccg caactggaga ccgatgccag
      121 cgccaatgat tacggtcagg ccgaaccgcg gatcgctaat gcccttgccg tcgccggtat
      181 gcctcccgtg aacgtgcatg cctggtcgtt tgctcccggt gagcatacgc tgacgctggg
      241 taagggtctt tgcctggtgc tgggttttgc gaaaccaatg ggtaagatca gcacctatga
      301 tgccggcgtc gacgaacccg gcagcaaaaa cctggactgg ttattcgaat aaagcatgaa
      361 gagaaaaatc atattgttcc tgttgtttat cggtcatatg gccgcggccc aggacctccg
      421 gctttggtat cgccagccgg caaagatatg gacggaagcc cttcccatcg gtaacggcaa
      481 tctcggcgcc atgatctatg gcgggatggc aaacgagcgc ctccagctca acgaatccac
      541 tttttggaca ggacagccgc gtagctatca gcgcgaggac gcctggcaat atctcgacac
      601 catccgtcaa cttttgttcg acggccgtca ggccgaagcg gaaactttgg cgcaggccca
      661 tttcatggga aagaagtttc ccgacgaaaa ggcttacgat ccgctgaaag ccgcgtggtt
      721 tcacaaagtg cggtcggaca cgtctttgat gagcgccgac gatagtcact ggccgacgat
      781 gaagataccg acccccgatg gatgggaggc cgcgggtctc gatggagtgg atggcgccgt
      841 ctggttcagg acgaccttcg atgtgccggc gggatggaag gggaaggatg tggtcctcga
      901 tctgggtcgt atccgggatg cggattttac ttatgtcaat ggggtattgg tggggcatga
      961 tgaaggcacc agcaagaagc gggtctacac catcagcgcg tcgatgttaa agccatacca
     1021 caatgagatc gccatccatg tactcaatta taacgataaa ggagggctca ccgggcggaa
     1081 aggagaaagc aaccttttct tgcgtcagga tggccaatcc atcgcattgc cgcacgattg
     1141 gaagtacgcc atccaggacg gaaaccctcc gcccatgccc caatacgagg cggcatatca
     1201 gcccttcggc gatctttacc tgaatttccc tgtataccag cccggggatt ataaacgcga
     1261 gctcgatcta aggaccgcca tcgcgaagac ctcctattcc gataacggcg tgcactatac
     1321 gcgcgaatat ttcgccagcg cgcccaatcg ggcgatcgtc atccatctca cggccgaccg
     1381 accgggtaag atcagttttc agctggccat gaagacgtcg gaacacgtca tgtccaatca
     1441 ccgtatcgac gaacatacct tcgcgttgat ggaaacggcc cataatggcg tgctgcacgg
     1501 agtagccttc gtccatgtgg aaacccgcgg cggacataat gaaggcaata tggtctccgg
     1561 cgcagacgag gcaaccatta cattggttgg agccaccagc ttcgtcaact atcacgatgt
     1621 ctccggcgat ccggaacgca tctgccgggc tcgtatcaat gcactgcggg gcttgtcatg
     1681 ggagagtctg aagaccgcgc atctgcagga atatcgcccc ttgttcaata ctttttcgct
     1741 gcggttgggt aagagtgata ctacgctgcc caccgatgag cgtatcctgc attatacgcc
     1801 ggggagcgac cccggcttta ttgcgttgta tgtgcaatat gcgcgttatc tcctgctgtc
     1861 ctcctcacgg cccggcgcct catacccggc caatctgcag ggtatctgga acgatcagct
     1921 cgatccgcca tggggcagca aattcactac caatatcaat ctggaaatga actactggcc
     1981 cgccgagccg ctccatctcg gcgaatgctc ggccccgttg ttccggctcc tgcaaaatct
     2041 ctcccaggcc ggaaaggcga cggcgaaaga ttattaccgc gctccggggt gggtgcttca
     2101 tcacaatacc gatctttggt gtggaacggc gccgatcaat gcctcgaatc atggtatctg
     2161 gccgaccggc ggggcctggc tctgtcacca gatatgggaa cattacctgt tcacgaagga
     2221 cgaaaaattc ctgcaacagt attatccgat catgcggagc tgcgcgacat tctttttgag
     2281 ctttctcatc aaagatccga aaaccggttg gctgatcagt acgcccagcg cctcgcccga
     2341 gcacggtggg ttggtggcag gacctacgat ggatcaccag atcatccgcg atctgttcaa
     2401 aaactgcata gctgcagagg ccattttgcg tcactacccg gaattcatga atgagctaag
     2461 agacaaatta tcgcggatcg cacccgacag gatcggccgc tacggacaat tacaggaatg
     2521 gctcgaagac aaagacgaca cgacggacac acaccggcat atctctcatc tatggggcgt
     2581 ctatccgggt accgatatca catggagcga cagcgcaatg atgcgggctg cgcgtcaatc
     2641 gctggtctat cgcggagatg aaggcaccgg ctggagcctt gcctggaagg tgaattgctg
     2701 ggcaaggttt agggatggcg atcatgcgct gcgtttggtc gataagttgc tttcttccgc
     2761 ggtgggcgct accggtgagc atggaggtgt ttatcctaac ctctttgact cgcatccgcc
     2821 tttccagata gacggcaact tcggtggtgc ggccggtatc gccgaaatgc tactgcaaag
     2881 ccaggatagt atcgtcgatc ttttgccggc attgccggca gtgcttgccc ggggcgcggt
     2941 caacgggatc tgcgcccgcg gcggttttga attggatatg cagtgggaga gcgggagatt
     3001 ggaaaatgtc gtcgtcctat cgcgtgcggg cggaccatgt gtcttacgct atcgcgggca
     3061 tgtggtgcgg ttgtccacga cggcgggaaa gaaatatcgt ctcaccggtg aattgaaagt
     3121 cttaaactga tgcgcctact gttcttctta ttaatgccga tggcggtact ggggcagcgc
     3181 cgcgatattc tactcgatgg cggttggacc tctgcgctca atggccggca ttgggttgcg
     3241 gtggacatcc cgcacaactg ggatgactat ggcggttatc gccggctccg tcatggcaat
     3301 ctgcatgggt atgcgctcta tcgccggaac ctcaccgtcg acaaaaattc cggtcttcgc
     3361 tattttcttt ggttcgaggg cgtcggctct tacgcgacgg tatgggtcaa tggtcggaaa
     3421 ctaggttatc atgccggtgg gcgtacctct tttacggtgg acattacgct ggcgctacgc
     3481 ccgggtcatg ccaccaatgt catcgatgtt cgggcggatc atccggcctt tatccgtgat
     3541 ctgccctggg tgtgcggcgg atgttcggat gaacgcggtt tttccgaggg ctcgcaaccg
     3601 atgggtatct ttcggccggt gcacctggtg atcactgcac ctacgaggat cgagccgttt
     3661 ggggtacacg tctggaacga cacgacgatc tccgaaagga acgcgaccct ctatctcgaa
     3721 acagagatac ataactacgg cacgacgcct gtcgaaggac tggtcataca gaaactgttc
     3781 gatgcacatg gcgcaccggt ggctattacc cagacacaac agcgtatcgc accaggccag
     3841 acggtaacgg tatcgcagca gctgaaagat atccccgatc cgcatctctg gtcgctcaac
     3901 acaccatatc tatattcact aaaaaccagt tttggtggcg atggagtggt cacgtcttac
     3961 ggcatccgcc gtatccactg gcccgacctg cacacaccgg gtccgcatcc ttttctactg
     4021 aacgggcggc ccgtcttcat caacggcatc gccggatatg agcaccgcat cggtaacagc
     4081 catgcgttta ccgcagaaga gatcagggcg cgtgtgcaac agatcagggc agcgggattc
     4141 aacgcttttc gcgacgcgca tcagccgcat aacctgttat accagcatta ttgggatagt
     4201 ctcggtatgc tgtggtggcc gcagttgtcc gcgcatatct ggttcgacac gccggcattc
     4261 cggcaaaatt tcaagacggc actcaccgaa tgggtgaaag agcgacgtaa cagtccttcg
     4321 gtgatcctct ggggattgca gaacgagagc aagctgcccg aagacttcgc acgcgagtgc
     4381 gtcgcgctga tcaggcaact cgatcccacg gcttcctcgc aacggctgat cgtcacctgt
     4441 aacggaggca caggcaccga ttgggatgtt ccgcaaaact ggacaggtac atatggaggc
     4501 gatccggcta cctacgccgc cgatcttcgt cggcaggtgc ttgtcgggga atatggcgca
     4561 tggcgcacga tcgatctgca tggcgatgtc cccaacagtg aagacaggat gtgccggctg
     4621 atggagacga agatccgtct cgccgacagc gtgaaagggg aggtggcagg gcatttcgca
     4681 tggatctttt cctcgcatga caatcccggc cgcgttcagt cgggcgaagg attgcgcgag
     4741 atcgaccgca tcggccccgt taactataaa ggattgctca cttcctggga ggaaccggtg
     4801 gatgcctatt atttgtatag aagtaatttc gtcgatgccg cgcgatcgcc gatggcttat
     4861 atcgtccgtg gcgtcagttc ccccattgtg gtgtattcca actgtgactc ggtcgaattg
     4921 ttcaacgacg tcgacagtat atccctgggg aaacgcacgc gaggcggtat cggaacccat
     4981 tttgaatggg atagtgcgga tatccggtac aatgtgctgt acgcggtcgg ttggtatcac
     5041 ggccggccgg tggtccatga ctgtactgtg ctggaccatc ttccgcgaag tccgcatttc
     5101 cgccgactgg ttggcggggc agagctgctc gccgctgccc cgggttacca ttatttatat
     5161 cgcgtcaatt gcggcggtcc cgcctatacc gatggcgccg ggcaggcctg gatggcagac
     5221 cggcattaca ccggtgggaa gacatggggc tcactctcat ggaccgacga ttacaaagga
     5281 ctacctccct ttttcgccag ccagcgctgg acggccgatc cgatcgcggg tacgggggac
     5341 tggccactct ttcagaattt caggtacgga ctcggtaaac tcgcatatac cttcccggta
     5401 caggatggag attaccggct ggaattgtat tttgcgggac cctggctggg ggcgggagga
     5461 ggcatggact gtacgggctg gcgtgtattc aacgtcgccg tcaacggaaa gaccgtgctg
     5521 cacgatctcg acatctggag agaagccggt cgtaaccggg cattgaaaaa ggttatccgg
     5581 gtgcatgtta ccggcggaaa actggtcctt tccttcccgc acgtcgcggc aggacaggcc
     5641 atcatcgacg cgctggcgat cgcgactacg gaccccggga tgacccccgc accgccttcg
     5701 gcgcccgaca cggcgaagat caggctggag ccggcctacg atctcaggcc ggtgacaagc
     5761 tataaaggcg actcgacgaa atgggatatc gaagtaggcg tgggggataa atattcgctg
     5821 accgtcaaat accgttggct gaagaccccg gtgaccgccc ggctggaact gcgcatggaa
     5881 gacggcacgc tgctgcattc cgagacggtg accctgcaga ccacgccgcc ggctaaatgg
     5941 aattatctga acaccacgac cggaacgatg atcaatgccg gtcgatatac tatccgcctc
     6001 atcgtcccct cgcgcgaagg gttgcgtgtg gacgagctgc aggtgcaata agtttacgat
     6061 gaaaagattt gttgccggca taatgttgtt gatagcgtct tccgcgatgg ggcaggagat
     6121 caaactgacg aatcttcgtg ccgatgcgcg cggaaatgat gaccgccgtc cttggttgag
     6181 ctgggagctc aggtcctcac accatgacgt cctgcaatcg gcgtatcgga tcatgctctt
     6241 cgacgatgcc ggcgcggtct gggattcgca caagcaggag tctgccgtca gcacgggtgt
     6301 cgtctatgac ggcgctgctc ttgcgtcggg gcatacctat tactggaagg cgatggtttg
     6361 ggaccggggt cgtaacgaca gctcgaaatg gagtaccgtg gatactttcc aaacgggatt
     6421 atttaccccc gccgactgga agggcgccgg gtggatcggc tatgaggcac tgcccgattc
     6481 tgcattgcgg ttgccgggga cggagaagaa gaaaggtcct taccgcgata cgctgccact
     6541 gttccgtaaa gaatttcggg tagggcggcc gctacaccgc gctacggcat tcatcagtgg
     6601 tctgggtcag ttcgagctgc gcatcaacgg agaaaagacg ggtgatcatt ttctcgaccc
     6661 cggctgggtc gattttgcga aaaaggcatt gtatgtggcg ctggacataa cgtcccggtt
     6721 gcatcaggga tcgaacgcgc tgggggtaat gctgggcaat ggtttctatt acattccccg
     6781 cgagcggtat cacaagctgc tcaccgccta tgggtacccg aagatgatcg cgcgcatcct
     6841 tctcgagtat gccgatggga gcagggagga tatcgttacc gatagcagct ggcgaaccgc
     6901 accgggtcct gtcacctata gcagcatcta tggcggcgaa gactatgacg cccgccggga
     6961 acggatcggg tgggatagac ccggtttcga cgacaaggcc tggcgcgttc cggttatcgt
     7021 gaacgggccg ccggcgctgg aagagcagac tgccgacccg ctaaaagtct tcgaacattt
     7081 cggcccccgg gatattgccc ggcgcagccc atcctcctgg gtatacgatc tggggcaaaa
     7141 tgcttcgggc atcattcgtc ttcgcgtctc gggcccggcc ggcgccgtga tgcgcatcac
     7201 gccggcggaa ttgatccggg gtgacagcgt caaccagacg gctacaggaa agccttacta
     7261 ctggcaatat attttaaaag gacaggggat agagacctgg cagccgcggt tcacttacta
     7321 tggttttcgt tatctgcaac tggacggcgc agtacccgaa ggtcagccca accccggcca
     7381 tcttcccgtc gtgtcggtgc tggagggact gcatacccgc aatgcgatgg agcgcaccgg
     7441 gaggttcacg tgcagcaacc tgcttttccg ccggatcgat tcgcttatcg actgggctat
     7501 tcaaagcaat gcggccagca tttttaccga ctgccctcac cgcgagaagc tgggttggct
     7561 ggaagaggca catcttatgg gcaactcgtt gcggtatagc tatgacatcg ccccgctgta
     7621 ccggaagatc gttcgagata tgatggatgc ccagacaccg gatggcctta tcccggagat
     7681 agcgcccgaa tataccgtct tcgacaaccc gttccgcgat agtcctgaat ggggcagcag
     7741 cagcatcct
//
LOCUS       DASYSV010000197         4437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_933146, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000197 DASYSV010000000
VERSION     DASYSV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933146"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /gene="thiL"
                     /locus_tag="VGQ51_06060"
     CDS             complement(<1..753)
                     /gene="thiL"
                     /locus_tag="VGQ51_06060"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEV9036161.1"
                     /translation="MNQLPVMPVANITNMSENRTEISSIGEFGLIEHLTANIEIKNVS
                     TLVGVGDDAAVIDHFGKQTVITTDLLLEDVHFDLLYTPLQHLGYKSVVVNLSDIYAMN
                     ATPTQLTVSIGISNRFSVEALDEFYEGVYSACETYGVDLVGGDTASSKKGFVISVTAI
                     GEVTPDRFVRRSTAQKGDLLCCTGDLGAAYLGLIFLEREKKIFLENPEVKADLENEKY
                     VIGRLLKPDARKDIIGFFQQSDVLPTAMMDISD"
     gene            complement(764..1546)
                     /locus_tag="VGQ51_06065"
     CDS             complement(764..1546)
                     /locus_tag="VGQ51_06065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV9036162.1"
                     /translation="MLKSTLIRAAEAGAAVMQKYFGGDFYISNKEGINNLVTEADHAS
                     EKAIFSVIKEDFPDHFLLSEEAGAIKMDSSYKWIIDPIDGTVNFAHGLPLCCVSIGLE
                     QEGEMILGAVYNPFIKEFFFAQRGYGATLNDRKISVSDETSVAKSCLVTGFPYTYLDQ
                     PNGPLEVFSRLIRGGVPVRRLGSAAIDLCWVASGRFDGFYEHKLNAWDSAAGFLIVQE
                     AGGRVSDFTGGAYSLYQPHIIATNGKIHDELIEVVNGRHATF"
     gene            complement(1565..1855)
                     /locus_tag="VGQ51_06070"
     CDS             complement(1565..1855)
                     /locus_tag="VGQ51_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV9036163.1"
                     /translation="MTLSLKQIEKISKALGDGNRLKILNYISDKGGCGECSALQEIIG
                     LAQPSVSHHVKILLESGLIRAAKEGRNYSYTLNNGLLNQYTDALRTLILTPC"
     gene            1909..3081
                     /locus_tag="VGQ51_06075"
     CDS             1909..3081
                     /locus_tag="VGQ51_06075"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9036164.1"
                     /translation="MILSVIIVNYRVRYFLELCLHSLQKTLMGIESETIVVDNHSADG
                     SPETLRSLFPSVRWVINDENVGFARANNQALQLANGKYILFLNPDTVVPENFARHCLS
                     FLEGMPGIGGLGVRMVDGSGRFLKESRRGFPSPWVAFCRLSGLSALFPKSRRFSTYYM
                     GYLPEDRTHPAPVLSGACLWVSRSILTDIGGFDEQFFMYAEDIDLSYRLERAGYINYY
                     LADTTIIHFKGESTRKDIRYVRHFYKAMSQFRRKHFRKGLPALLNFAAEIAIWCRAAI
                     VGLIRVIGIGLAGLLGQDPKPGAGPIRTLVTGDPAETRLVAAALVGQRIIVTGQSGAD
                     EIIICQGRDFSFRQSIAELEADAPYRHGRQVKFHAAGSSSATGSPDPNGRGETLIF"
     gene            3200..>4437
                     /locus_tag="VGQ51_06080"
     CDS             3200..>4437
                     /locus_tag="VGQ51_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEV9036165.1"
                     /translation="MAKALRLPQKSPGRQQLKVLRKLLKKARFTEFGQRYRFDELLLD
                     RHPGKKFQQVVPTFDYNKIYAAWWNQTLEGKADICWPGKIKYYALSSGTSEAGSKYIP
                     VTTDLLRGNRIVMVKQLLTLRTYRDIPVKSIGKGWLMIGGSTSLQKGAGYYSGDLSGI
                     TARKVPFWFQPFYKPGRKVARTKDWTKKLEEVVRLAPQWDIGFLVGVPGWIQLCMEKI
                     IERYQLKNIHEIWPNLGFFVHGGVSFDPYRKGFEKLLGKPITYIETYLASEGFIAYQD
                     AQNSKGMRLVTNQHIFLEFVPFDNKNFDADGNLVENPEAYMIHEVEEGRDYALLLSTS
                     AGAWRYLIGDTVRFVDKERCEIIITGRTKHFLSLVGEHLSVDNMNKAVQRVAEEMNLR
                     IPEFTVAGIPHGLFFAHQWWL"
BASE COUNT         1032 a         1158 c         1211 g         1036 t
ORIGIN      
        1 gtcgctgatg tccatcatcg ccgtgggcag aacatcggac tgctggaaga acccgatgat
       61 atccttgcgc gcatcgggtt tgagcaaccg gccgatgaca tatttctcgt tttccagatc
      121 ggccttgact tcagggtttt cgaggaagat ctttttctcg cgttccagaa agatcagacc
      181 gagataggcc gcgcccagat cacccgtaca gcaaaggaga tcgccttttt gcgccgtgct
      241 gcgacggacg aaacggtccg gggtgacctc gccgatcgcg gtcacggaaa tgacgaaccc
      301 ctttttggaa gaggcggtat cgccgcctac caggtcgacg ccataagttt cacaggcgga
      361 ataaacgccc tcatagaact catcgagggc ttctacgctg aatcggttgc tgataccgat
      421 gctcaccgtc agctgggtgg gggtggcatt catcgcgtag atatcgctca aattcacgac
      481 gacggattta tagccgagat gctgcagcgg cgtatagagc agatcgaagt ggacgtcttc
      541 aaggaggaga tcggtggtta taaccgtctg cttaccgaaa tgatcgatga cggcagcatc
      601 atcgccgact ccgacgagcg tcgagacatt ctttatttct atgttggccg tgagatgttc
      661 gatcaaaccg aactccccga tagaagagat ctctgttctg ttttcgctca tattggtgat
      721 gtttgccacc ggcatgaccg gcagttggtt cattggcctc cgtttaaaaa gtggcgtgac
      781 ggccgttgac cacctcgatc agctcgtcat ggatcttccc gttggtggcg atgatatgcg
      841 gctggtaaag ggaatacgcg ccaccggtga aatccgagac ccgcccgccc gcttcctgca
      901 cgatcaaaaa acctgccgcg ctatcccagg cgttcagctt atgctcataa aacccgtcaa
      961 acctgcccga agccacccag caaagatcga ttgccgcaga cccaaggcga cggacgggga
     1021 caccacccct tatgagacgg gaaaaaactt ccaacggtcc gttaggctga tccagatagg
     1081 tatagggaaa accggtgacg agacaggact ttgccacgga ggtctcatcg ctgacactga
     1141 tcttccggtc gttgagcgtg gcgccatagc ccctttgtgc gaaaaagaac tctttgatga
     1201 aggggttgta cacggcgccc aggatcattt caccttcctg ctccaagccg atagaaacgc
     1261 aacaaagcgg aagaccatgc gcgaaattca cggttccgtc tatcgggtcg atgatccact
     1321 tatagctgga gtccatcttg atggccccgg cttcttcgct tagcaggaag tgatcgggaa
     1381 aatcttcttt tattacgcta aagatggctt tctccgaagc atgatcggct tccgtaacca
     1441 ggttgttgat gccttccttg ttggatatat agaaatcacc cccgaaatat ttctgcatga
     1501 ccgcggcgcc ggcttccgcc gcccggatga gtgttgattt tagcatgatg tacgataatg
     1561 gaatttagca cggtgttaag ataagcgttc gcaatgcatc ggtgtactgg ttcagcaggc
     1621 cgttattgag ggtgtagctg tagttcctcc cttcttttgc cgcccggatc agtcccgact
     1681 ccagcaatat ctttacgtgg tgtgagaccg agggttgcgc aagaccgatg atctcctgca
     1741 acgccgaaca ttctccgcag ccgcctttgt cggatatgta attcagtatt ttcaggcggt
     1801 ttccgtctcc gagggccttg gatattttct cgatttgctt caggcttagc gtcatctcat
     1861 tgtattgacg gatgcaaatc tatcaatttt ttggcataat ttgcccacat gattttatcc
     1921 gtcattattg tgaactaccg ggtaagatat tttttggagc tgtgccttca ctcgctgcaa
     1981 aagaccttga tgggaataga atcggagacc atcgtagtcg acaaccattc tgccgatgga
     2041 agtccggaaa cgctgcggtc gctgttccct tccgtaagat gggttatcaa tgacgagaat
     2101 gtcggatttg caagggcgaa taaccaggcg ttgcaattgg cgaacggtaa gtatatcctt
     2161 tttctcaatc ccgataccgt ggtccccgag aactttgccc gccattgcct ttcctttctc
     2221 gaagggatgc ccggcatcgg ggggctcggg gtcaggatgg tggatggaag cggtcgtttt
     2281 ctgaaggaat cgcgaagggg atttcccagt ccgtgggtag ccttttgccg tttgtccggt
     2341 ttgtcggcgc tgtttccgaa aagccgccgg ttctccacgt actatatggg ctatttgccg
     2401 gaggatagga cgcaccccgc accggtactg tccggcgctt gtctttgggt ttcccggtcg
     2461 atcctgacag acatcggcgg gttcgacgag caattcttta tgtatgcgga ggacatcgat
     2521 ctcagctatc gtctggaaag ggcaggctat atcaattatt acctcgcaga tacgaccatc
     2581 atccatttca agggggagag cacccggaaa gacatacgct atgtgcgaca cttctacaag
     2641 gccatgagcc agttcagacg caagcatttc cgtaaaggtc tcccggccct gctcaatttt
     2701 gccgccgaga tcgccatctg gtgccgggcg gccatcgtag ggctcatcag ggtcatcggt
     2761 atcggactgg ccgggctgct cggccaggat cccaaacccg gagccggccc gatccgaacc
     2821 ctggtaaccg gcgatcctgc ggaaaccagg cttgtagccg ccgccctggt gggacagcga
     2881 atcatcgtaa ccgggcagtc cggagcggat gagatcatca tttgccaagg acgggatttc
     2941 tctttccgtc aaagtatcgc agagctggaa gcggatgcgc cttaccggca tggacggcag
     3001 gtgaaattcc acgcggccgg cagcagctcg gccaccggca gccccgatcc caacggccgg
     3061 ggcgaaacgc tgatcttcta aacccccgtt cacagcggcc gaaaagcggc aaaccgaaat
     3121 aaattgttaa tttcgccgga acaccaaacc acgcagacac tacttaatgg cgttgattga
     3181 aattcaatta cctaaatcgt tggcaaaggc tttgcggctg cctcagaaat ctccggggcg
     3241 gcagcaattg aaggtattac gcaaattgtt gaagaaggcg agatttacgg aattcggtca
     3301 gcgatatcga ttcgatgagc ttcttctcga ccgccatccc gggaagaaat tccaacaggt
     3361 ggtacctacc ttcgattata ataagatata tgcagcctgg tggaatcaga cactggaagg
     3421 gaaagcggat atctgctggc cgggaaagat caaatattat gctttgtctt ccgggacatc
     3481 ggaagccggc agtaaatata ttccggtcac gaccgatctg ctccgcggca accggatcgt
     3541 gatggtcaaa cagctgctga cattgcgtac ctatcgcgac atcccggtga aaagcatcgg
     3601 caagggctgg ctgatgatcg ggggcagcac cagccttcag aaaggcgcgg gatattattc
     3661 cggcgatctc agcgggatca ctgcgcgtaa ggtgccgttc tggtttcagc cgttttataa
     3721 gccggggaga aaggtcgccc gcaccaaaga ctggacaaag aaactggaag aagttgtccg
     3781 gctcgctccg caatgggata tcggcttcct ggtaggagta cccggctgga tccagctgtg
     3841 catggaaaag atcatcgagc ggtaccagct gaaaaatatc catgagatct ggcccaatct
     3901 cggattcttc gtacatggcg gtgtctcttt cgatccctac cggaaaggat tcgaaaagct
     3961 gctcgggaag cccatcacct atatcgagac ctatctggca tcggaaggat ttattgcgta
     4021 ccaggatgcc cagaattcga aaggcatgcg cctggtcacc aatcagcata tctttcttga
     4081 attcgtcccg ttcgacaaca agaatttcga cgccgacggg aacctggtcg aaaacccgga
     4141 agcctatatg atccatgagg tcgaagaagg cagggactat gcgttattgc taagcaccag
     4201 cgccggcgca tggcgctatc tcatcggtga cacggtccgg ttcgtggata aagagcgttg
     4261 cgagatcatt attacgggaa ggacgaaaca tttcctaagc ctggtgggtg agcatctgag
     4321 cgtcgacaat atgaataaag ccgtgcagcg ggtggccgaa gagatgaacc tgcgcattcc
     4381 cgaattcacc gtggcgggta tcccacacgg tctgttcttt gcacatcaat ggtggct
//
LOCUS       DASYSV010000198         6081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_161461, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000198 DASYSV010000000
VERSION     DASYSV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161461"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VGQ51_06085"
     CDS             <1..782
                     /locus_tag="VGQ51_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9036166.1"
                     /translation="PHIYLGTISNVSRLQEGDSIPITYKEVSIGSGVLDILNSLGDSA
                     VGHLQDISFNPIDHQIYTTLPTTAAVASPAQIGHFDPSATHPVLNVITPTTTNPPIAN
                     MCGMFSGAAGDLFILTTDGRFYKGDPGTGEISLITRTALPVLGNDLRGDMASCVPRST
                     TANVQSGVSNDADPNGGLHIAPNPVASGQITVSVNSQGNDQARLQIIGPTGSPLKTRN
                     IQLIPGSNQFQLDVSQLNQGVYSVILYCTSGRVSTTKFVRL"
     gene            complement(777..3095)
                     /locus_tag="VGQ51_06090"
     CDS             complement(777..3095)
                     /locus_tag="VGQ51_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEV9036167.1"
                     /translation="MEPVYIQKIAEKLSFSIKQINNIFGLQAEGATIPFMARYRKEAT
                     GNMDEVQINDVVEQIKYFTELDKRKETVLKTIEGLGKLTPEIKTRVEGCFDATELEDI
                     YLPYKPKRKTRASAAMEKGLEPLAKLLFEQGDGDPATAAGAYINEQVKDVKEALQGAR
                     DIIAEWISENEQTRARIRQLFTREASVSSKVITAKKEEEEAQKYRDYFDFSGSLADSP
                     SHRILAIRRGEKEGFLIMDLDIDKQMAVDDLERIVVKNATPAAAEVKRAVEDSFDRLL
                     KPSIENEFRLLSKNRADEEAIHVFAENLRQLLLASPLGSKRVLALDPGFRTGCKLVCL
                     DAQGNFVHNSTIYPHPPQNEAGASIAQLKTLVDRYNIEAIGIGNGTAGRETEQLVRGI
                     DFGRHVSIFMVNESGASIYSASEVAREELPDQDVTVRGAVSIGRRLLDPLSELVKIDP
                     KSIGVGQYQHDVNQTKLKEELDRVVESCVNHVGVDLNTASKHLLTYVSGLSGTLAKNI
                     VEFRAKNGAFTTREDLKKVSLMGPKTYEQCAGFLRIAGAANPLDNSAVHPESYRIVET
                     MAADLNCSVDELIRQEELRKKIVRKKYISETAGEFTINDILKELEKPGRDPRSTIEEF
                     RFEESIKTMEDLKPGMTVPGIVTNITAFGAFVDIGVKQDGLVHISQLSNTFVSDANSV
                     VKLQQKVMVTVLEVDTARKRISLSMKGNSPAKGDQAMKGRTGAAGNKKPMGDNKKPAA
                     MGPQAGKVGKKEEEPDAFQAKLLALKKKFKDS"
     gene            3150..4520
                     /locus_tag="VGQ51_06095"
     CDS             3150..4520
                     /locus_tag="VGQ51_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV9036168.1"
                     /translation="MDIKNNILETIGHTPLIRFNKLTRDLPCTVLGKIEYFNPGNSAK
                     DRMALKMVEVAEKEGRLKPGGTIIECTSGNTGMGLALAAVVKGYKCIFTTTDKQSKEK
                     IDILKALGAEVIVCPTNVEPADPRSYYSIARRLAKEIPDSFHANQYDNLANRLAHYET
                     TGPEIWEQTDGKLTHLVVATGTGGTVTGTAKFLKEKNPKIQVWAMDPYGSLLTKYFRT
                     GNIDMNEVHPYVTEGIGEDFVPQNYDMQYVDVFEQVTDKDAAIMTRRIAREEGFFCGY
                     SAGTAVQGLLQLNHLLKKGDMVVVILHDHGSRYVGKVYNDQWMIERGFLAVKTFRDII
                     SGRGGAKRLISTNPDNTVGEAFQLMQKYHIENLPVLKDGEPVGAISESGLFNRLMNNG
                     SDIKGKKIAEVLEKPYPVVSFDTPVERLSTLISKDNGAVLGRDDSGDYHIVTKYDVIQ
                     ALSPIS"
     gene            4902..5714
                     /locus_tag="VGQ51_06100"
     CDS             4902..5714
                     /locus_tag="VGQ51_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEV9036169.1"
                     /translation="MPSFTDQLNRTICLPQTPRRIISLVPSQTELLFTLGLDETVVGI
                     TKFCVHPETWYHNKPRIGSTKAIDTARVDALRPDLIIAAKEENEKPQIEALASRYPVW
                     ISDVKRLSDAIDMIHAVGRLTDTSGKAMTLADEIGREFATLPGSSPLPAASAPPFPPA
                     AYFIWRNPWMAAGRDTFINDMLYHCGFSNAFANHDRYPTIRLPDLAGRPSPIILLSSE
                     PYPFRNRHIDEVREILPDAMIHLVDGEFFSWYGSRLLQAPAYFRNLRQQIMA"
BASE COUNT         1358 a         1931 c         1390 g         1402 t
ORIGIN      
        1 taccccatat ctatctcggt acgatatcca acgtttcaag actgcaggag ggagactcta
       61 tcccgatcac atacaaagag gtatccatcg gatccggtgt gctggatatc ctgaacagcc
      121 tgggagattc cgccgtagga cacttgcagg atatttcgtt caaccccatc gatcaccaga
      181 tctataccac tcttccgacg acggcagccg tcgcctcccc cgcacagatc ggacattttg
      241 atccatcggc cacacacccg gtactcaatg ttatcactcc gacaactacg aatcctccca
      301 tcgcgaatat gtgcggaatg ttctccggag cggctggcga tctcttcatt ctgaccaccg
      361 acggaagatt ctataagggc gatcccggta ccggggagat cagtttgatc acccggacgg
      421 ccttgccggt gctgggcaac gatttgaggg gagatatggc ttcctgcgta ccgaggagca
      481 caacagccaa cgttcagagt ggtgtctcca acgacgcgga tcctaatgga ggcttgcaca
      541 tcgctccgaa tcccgtggca tcgggtcaga taaccgtatc ggtcaattcg cagggcaacg
      601 accaggcaag gctgcagatc atcggcccca ccgggagtcc cctcaagacc aggaacatcc
      661 agctgatccc gggttccaac cagttccagc tcgatgtgtc gcaattgaac cagggcgtct
      721 attccgtgat cctgtattgt acatcgggcc gcgtcagtac caccaaattc gtccgtctat
      781 gagtctttga atttcttttt cagtgccagt agcttagcct gaaatgcatc gggctcctcc
      841 tccttctttc cgacttttcc agcctgcggt cccatagccg caggcttttt attatcgccc
      901 atgggctttt tattccctgc agcgccggtc ctccccttca tcgcctgatc ccccttcgcc
      961 ggactattcc ccttcatcga cagcgatatc cgcttcctcg ccgtatccac ttccaatacc
     1021 gtgaccatta ctttctgctg cagcttgact accgaattgg cgtcggatac gaaggtattg
     1081 ctcagctggg aaatatgaac cagcccgtcc tgcttgacgc cgatgtcgac aaacgccccg
     1141 aaggcggtga tgttcgttac gatacccggc accgtcatac ccggcttcag gtcctccatg
     1201 gtcttgatgc tctcttcgaa acggaattct tctatcgtac tacgcgggtc ccgccccggt
     1261 ttctccagct ctttcaaaat atcgttgatc gtgaattcgc cggcggtctc gctgatatat
     1321 tttttccgga cgatcttttt acgcaattct tcctgccgga tcagctcgtc gaccgaacag
     1381 ttcagatcgg ccgccatggt ctccacgatc cgatagctct cgggatgcac cgcactattg
     1441 tccagaggat tggcggcccc ggctatccgg aggaatcccg cgcactgctc gtaagtcttg
     1501 ggacccatca gactcacttt tttcaggtct tcccgggtag tgaaggcgcc gttcttcgcc
     1561 ctgaactcca cgatgttctt cgccagcgtt ccgctaagcc ccgatacata ggtcagcagg
     1621 tgcttgctgg cggtattcag atccacgccg acatggttca cacaactttc caccacacgg
     1681 tccagctctt ctttcagctt ggtctggttg acgtcatgct ggtattgccc tactccgatg
     1741 gattttgggt ctatcttcac cagctcgctc aacggatcga gaagacgacg cccgatgctc
     1801 actgctccac gcacggttac atcctgatct ggaagctctt ccctggccac ctcgctcgca
     1861 gaatagatcg acgcgccgct ctcattgacc atgaaaatgc ttacatgacg tccgaaatcg
     1921 atgcccctga ccagctgctc cgtctcgcgt cccgccgtac cgttcccgat gccgatggct
     1981 tcgatgttat accggtccac cagggtcttg agctgcgcga tgctggcccc cgcctcgttc
     2041 tgcggcggat gcggatagat agtactgtta tgcacgaaat taccctgggc gtccaggcag
     2101 accagcttac atcccgtccg gaaccccggg tccaatgcaa gcacacgctt ggaacccaag
     2161 ggcgaagcca gcagcaattg ccgcagattc tcggcaaaaa catggatagc ttcctcgtcg
     2221 gccctattct tgctgagcag ccggaactca ttctcgatgg aaggtttcaa cagccggtcg
     2281 aacgaatcct ctacggcgcg cttcacctcc gccgccgcgg gcgtcgcatt cttcacgacg
     2341 atccgctcca gatcatccac cgccatctgc ttgtcgatat cgaggtccat gatcaggaag
     2401 ccctcttttt ccccgcgacg gatcgccagt atcctgtgcg aggggctgtc cgcaagcgaa
     2461 cctgaaaaat caaaataatc ccggtatttc tgggcttcct cctcctcttt cttagcggta
     2521 atgaccttgc tgcttaccga cgcctcgcgg gtgaacagct gtcggatcct ggcacgggtc
     2581 tgctcgttct ccgaaatcca ctcggcgatg atatccctcg cgccctgcaa cgcctctttg
     2641 acgtccttta cctgctcgtt gatataggct cctgctgccg tcgcggggtc gccgtcgccc
     2701 tgctcgaaca acagtttcgc caaaggctcg agcccctttt ccatggcagc ggacgcccgc
     2761 gtctttcgct tcggcttata aggcagataa atatcctcca gttcggtggc atcgaaacaa
     2821 ccttcgaccc ttgttttgat ctccggcgtc agcttgccca acccctcgat ggtcttgagc
     2881 accgtttctt ttcgtttgtc cagctccgtg aaatatttga tctgctcgac cacgtcgttg
     2941 atctgcacct cgtccatatt accggtggct tccttccggt accgcgccat gaaggggatc
     3001 gtcgcccctt cggcctgcag accgaagatg ttattgattt gcttaatgct gaaagacaac
     3061 ttttcagcga tcttttgaat ataaacaggt tccatgggta attccgtttc tgcgggcggc
     3121 aaatgtaggc aaaatcctac ctttatgcca tggatatcaa aaacaatatt ctggagacga
     3181 tcggacacac tcccctcatc cgtttcaaca aactgacgcg ggatcttccc tgcacggttt
     3241 taggcaaaat agaatatttc aaccccggca attccgccaa agaccgcatg gcgctgaaaa
     3301 tggtcgaagt cgccgaaaaa gagggcagat taaaacccgg cggaaccatc atcgaatgca
     3361 cttcaggcaa caccggcatg ggtctggcgc tcgccgcggt cgtcaagggt tataaatgca
     3421 tctttactac gaccgacaaa caatccaagg aaaagatcga tatcctgaag gcattgggcg
     3481 ccgaagtgat cgtttgtccg acgaacgtgg aaccggccga cccgcgttct tactattcca
     3541 tcgcgcgacg tctggcaaaa gagatccctg attctttcca tgccaatcaa tacgacaacc
     3601 tcgccaaccg cctcgcgcac tacgagacca ccggtcccga aatttgggag cagaccgacg
     3661 ggaaactcac ccatctggtc gtagccaccg gtacgggcgg caccgtcacc ggcaccgcca
     3721 aattcctcaa ggagaaaaac ccgaagatcc aggtttgggc gatggacccc tacggctccc
     3781 tcctcaccaa atatttcagg acggggaaca tcgatatgaa cgaggtacac ccctacgtca
     3841 cggaaggcat cggcgaggac ttcgtaccgc agaactacga catgcagtac gtcgatgtgt
     3901 tcgagcaggt gacggacaaa gacgccgcca tcatgacccg ccgcatcgcc cgcgaagaag
     3961 ggtttttctg cggctattcc gcgggcacgg ccgtacaggg cctcctgcag cttaatcatc
     4021 tgctgaagaa aggcgatatg gtcgtcgtca tcctccatga ccatggcagc cgctatgtcg
     4081 gcaaagtcta caacgaccaa tggatgatcg aacgtggctt tcttgccgta aaaaccttca
     4141 gggacatcat atccggacga ggcggcgcca aacgcctcat ctcgacgaac cccgacaata
     4201 ccgtgggtga ggcattccag ctaatgcaaa aataccatat cgaaaacctg cccgttctga
     4261 aggacggaga gcccgtcggc gccatcagcg aaagcgggct tttcaaccgt ctcatgaaca
     4321 atggttccga tatcaaaggg aaaaagatcg cggaagtcct cgaaaaacca tatcccgtgg
     4381 tttccttcga caccccggtg gaaagactta gtactttgat cagtaaagac aatggcgccg
     4441 tgctgggccg cgacgacagc ggcgactacc acatcgtcac caaatacgac gtcatccagg
     4501 cgctgtctcc catatcgtag ttttacgata gtgcggcgta attgctgcaa cgccgaaata
     4561 aagcaaaacc tctcttgtcc ggcgcgtttt aacaatttat ttttggttca gcatttaacg
     4621 atcgacccta ccccgatatc cttaagaaaa gctatctaaa tcctttaacc gcgaagaacc
     4681 cgataaccgc caacagcgga ttatcgataa gccctaatcc ataatcctat attaaggcct
     4741 gaaagatgcc ctaaagacta aacttattcc gagatccaac gtccgaaaaa accacaaaaa
     4801 gcagtaccat ccgcatcaaa actgctcaac tcccggccct cctgctaagt acctgattag
     4861 agcatttcgt ttatttagca ggaggccttc atcgatggcc catgccttcc tttaccgacc
     4921 aattgaatag aacgatctgt ctaccgcaga ccccacgccg gatcatttcc ctcgtccctt
     4981 cacaaactga actccttttc accctcggtc tcgatgaaac agtcgtcggc atcaccaaat
     5041 tctgcgtaca tcccgaaacc tggtaccata acaaacctcg catcggcagc acaaaagcca
     5101 tcgacaccgc ccgcgtcgac gccctccggc ccgacctcat catcgcggct aaagaggaga
     5161 acgaaaaacc ccagatagag gccctcgcct ccaggtatcc tgtctggatc agcgacgtca
     5221 aacgtctttc cgatgccatc gatatgatcc acgccgtcgg ccggctcacc gacacctcgg
     5281 gcaaagccat gaccctggcc gacgaaatcg gccgcgaatt cgcgaccctt ccaggctctt
     5341 cacccctccc ggccgcgtcc gcgcccccat ttccccccgc cgcctatttc atctggcgca
     5401 atccctggat ggcggcaggc agagacacct ttatcaatga tatgctttac cattgcggtt
     5461 tctccaacgc cttcgcgaac catgatcgct accccaccat ccgtctgccc gatctggcag
     5521 gccggccgtc tcccatcatc ctgctatcct ccgagcccta ccccttccgc aaccgccata
     5581 tcgatgaggt ccgcgagatc cttcccgacg caatgatcca ccttgtcgac ggcgaattct
     5641 tcagctggta cggtagccgc cttctgcaag ccccggccta tttccgtaac ctgcgccaac
     5701 agatcatggc ctgaccccac cgggcccgct atcgccgtca acccggcgcc tgcgtacaac
     5761 tacgtaccca cttcgttgta ttgttcggct gcaaaaacca tcttacattt aactctaccc
     5821 gctttataac tatctccacg gaaagactcc gttatcttgt tacccaccct acccgatttt
     5881 ccgaccattt tccatattct tttccatgag cgcatctatt cacaccaatg cctccctccg
     5941 gaaaatgatc agcgttttgc tgctcatcgg gttgctgtcg ctgttgctgg tgctatattt
     6001 cctgaaatat gtccccgacc agcgcaacga attccaccgc caggcttttc tcgagcttgg
     6061 ccagatccaa aaagcactgc a
//
LOCUS       DASYSV010000199         4273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_37147, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000199 DASYSV010000000
VERSION     DASYSV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37147"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VGQ51_06105"
     CDS             <1..204
                     /locus_tag="VGQ51_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HEV9036170.1"
                     /translation="VSLAPGGSVRLHFTLTSQQLSVVDEKGLSSVPAGKIKISVAGSL
                     PGARSEQLGAAKAVQTEVEVAGK"
     gene            212..1393
                     /gene="uxuA"
                     /locus_tag="VGQ51_06110"
     CDS             212..1393
                     /gene="uxuA"
                     /locus_tag="VGQ51_06110"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEV9036171.1"
                     /translation="MRRTYLMEETMRWYGPKDPVSLWDIRQSGCTGVVTALHHIPVGE
                     VWTMEEIGKRKAEVEAAGMRWTVIESLPVHEEIKKQTAGYKQWIANYQESLRNVAKCG
                     LEVVTYNFMPILDWMRTDIAYEMPDRSRALRFEKSAFIAFDLFLLRRPGAEKDYTPEE
                     IAKAERRLGTMSVAEKETLYRNTLLGLPGSEERFTEEQILAALKTYKDIDASRLKQHL
                     FHFLQQVVPVAEEVGLKMAIHPDDPPFPILGLPRIVSTEQDARELLDCAPSPANGLCF
                     CTGSFGVRADNDLAGMVRRLGDHIHFIHLRSTLRDAEGNFFEADHLDGDVDMVGVIRE
                     LLEVQRQRGIAIPMRPDHGHQMLDDLHKTTYPGYSAIGRLRGLAELRGVQRALAAVMP
                     R"
     gene            1494..3086
                     /locus_tag="VGQ51_06115"
     CDS             1494..3086
                     /locus_tag="VGQ51_06115"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV9036172.1"
                     /translation="MPRTFFCCFSLFFACVAFGQRPTYGGKLKPEQANMDVKHYTIDL
                     ALDIPNHSVAGYAIVRADLKQPATGLLLDLMDSFRVSKVLVNGKTATFDHSAHELHIT
                     SDKPWPAGPVTVRVEYAGHPMIAKRPPWDDGFTFTTDSTGHPWVAVTAEGTGGKIYFP
                     AKDHPSDEPDEGVDMTITVPKGLVVAGPGLLQGVKNRGNQSSFHWKTNYPINNYSIVF
                     NVGDYVVVSREYTTIDGHKVPMQFYVLKEHADKAPHQLELLEHFARVKEKYFGEYPWV
                     KEKIGIVETPHLGMEHQTMNAYGNKFRYTVVGGQDFDGLMNHEFGHEWWGNKVTAGDW
                     ADYWIHEGIGTFGDALYTRDMEGEAAYEKYFQRIAPRIRNDKPVVLGKDIPEDEAYHP
                     DIYPKGAFFMHTIRYVIGDSIFFSTLKHLATDSNTTYTHTTSTADVERLFSGASGIDL
                     GPLFHLFLYTTDKLEIDVRQTGETRYLVRLQNIDMPLPLEIVTDKGSQRVMVDKKGVV
                     INSQSLPLVDPRVFYLKKVIYE"
     gene            3079..>4273
                     /locus_tag="VGQ51_06120"
     CDS             3079..>4273
                     /locus_tag="VGQ51_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEV9036173.1"
                     /translation="MNKAMFDKTVYRARRAVLKATVGKGMILLLGNEESSMNYRDNLY
                     PFRQDSSFLYYFGLDKPGLTGLIDIDNDREFIYGRELTMDEIVWTGPQPTLAEQAEAA
                     GIGETRAVTTLSSDIASARGAGRTIHFLPPYRPENISKLSGWLGIPGGGVASHASVPL
                     IKVVIGQRSVKTPEEIEQIDQAVDTTAEMHLAAIEGCRDGMTEAQLAGRLQAIAISAG
                     GNLAFPTILTVNGQVLHNHYGPTVMREGRLAICDAGAENAMHYAGDMTRTFPVGKKFT
                     PRQREMYDIVLKAQESAAQALRPGVLFRDVHALAARVLVEGLKDAGVMKGDPAEAVAA
                     GVHTMVFQCGLGHMMGLDVHDMEDLGEEYVGYTETLKKSKEFGWKSLRLARALEPGFV
                     VTVEPG"
BASE COUNT          960 a          995 c         1397 g          921 t
ORIGIN      
        1 gtatctttgg cccccggtgg ttcggtgagg ttgcatttta cgctgacgtc gcagcagctg
       61 tcggtggtgg atgaaaaggg gttgtcgtct gtgcctgcag gaaaaataaa gatcagcgta
      121 gcgggatcgt tgccgggtgc caggagcgag cagcttggcg cggcgaaggc ggtgcagacg
      181 gaggtagagg tggcggggaa ataaaaaatt tatgagaaga acatatttga tggaagagac
      241 tatgcggtgg tatggcccta aggatccggt aagcctatgg gatatccggc agtccggttg
      301 taccggtgtc gtaacggccc tgcatcatat accggtgggt gaagtgtgga cgatggagga
      361 gatcgggaag cggaaagcgg aggtcgaagc ggcaggcatg cgctggacgg tgatcgaaag
      421 cctgccggtg catgaagaga tcaagaagca gaccgccggt tataaacagt ggatcgcgaa
      481 ctatcaggag agtctgcgga atgtggcaaa atgcggtctg gaggtcgtga cgtataattt
      541 catgccgata ttggattgga tgcggaccga tatcgcctat gagatgccgg acaggagcag
      601 ggccctgcgt tttgaaaaat cggcgttcat agcttttgat ctgttcttat tgaggcgacc
      661 gggggcggag aaagactata cgccggagga gatcgcaaag gctgagcggc gactcggcac
      721 gatgagcgtg gccgagaagg agacgttata ccgcaatacg ctgttgggtc tgcctggcag
      781 tgaagagcgg tttaccgaag agcagatcct cgccgcgctg aaaacttata aagacatcga
      841 tgcgtcccgg ctgaagcagc atttgttcca ttttttgcag caggtggtgc ctgttgcgga
      901 ggaggttggg ttgaagatgg cgatccatcc tgacgacccg ccgttcccta ttttgggatt
      961 gccgcgtatc gtcagtaccg aacaggatgc gcgcgagttg ttggattgcg ccccgtctcc
     1021 cgcgaacggt ctttgttttt gtacgggcag ctttggtgtc cgggcggaca atgatctggc
     1081 cgggatggtg cgcaggcttg gggatcatat ccatttcatt catttgcgaa gcacgctacg
     1141 ggatgcggaa ggcaattttt tcgaagccga ccatctcgat ggcgatgtag atatggtggg
     1201 agtcatccgg gagttgctgg aggtgcagcg ccagcgtggg atcgcgatcc cgatgcgtcc
     1261 cgatcatggg catcagatgc tggacgatct gcataagacg acctacccgg ggtatagtgc
     1321 tatcgggcgg ctgagggggt tggcggagct gaggggtgtg caacgggctc tggcggcggt
     1381 tatgccccgg tgaggaggac cgcgtaaggc ggcgcgtggt tgcaaaaggt taattaacgg
     1441 ctaggcgacc tgcgtccgta tccgtagatt gtggcttcta tcaactctac gctatgccaa
     1501 gaaccttttt ctgctgtttt tccctctttt tcgcgtgtgt ggcattcggg cagcggccga
     1561 cgtatggcgg caagctgaag cctgaacagg ccaatatgga cgttaagcat tacaccatcg
     1621 acctggcgtt ggacataccg aaccacagcg tcgccggata tgcgatcgtc cgggccgatc
     1681 tgaagcagcc ggccaccgga ctgcttttag atctgatgga ttctttccgg gtcagcaaag
     1741 tgctggtcaa cggcaagacc gctactttcg atcattcggc gcatgagcta catatcacat
     1801 cggacaaacc ctggccggcg gggcccgtca ctgtcagggt cgaatatgca ggacatccga
     1861 tgatcgccaa acggccgccg tgggacgacg gttttacttt tacaacggac tctacgggtc
     1921 atccgtgggt tgccgtcacg gcggaaggca cgggaggtaa gatctatttt cctgccaagg
     1981 accatccttc cgatgaaccc gatgagggcg tggatatgac gatcacggtc cctaagggat
     2041 tggtcgtcgc cggtccgggt ctgctccagg gcgtaaagaa ccgggggaat caaagcagct
     2101 ttcattggaa gacgaattat ccgataaaca actacagcat cgtattcaac gtaggcgact
     2161 atgtcgtcgt gagccgtgag tatacgacca tcgatggaca taaggttccg atgcagttct
     2221 atgtgttgaa ggagcatgcg gacaaggcgc cgcatcagtt ggaattattg gagcatttcg
     2281 cccgcgtcaa agaaaaatat ttcggcgaat atccctgggt gaaggaaaaa atagggatcg
     2341 tcgaaacgcc gcatctgggt atggaacatc agacgatgaa tgcctatggc aataaattcc
     2401 ggtatacggt tgtgggcgga caggattttg acggcctgat gaatcatgaa ttcggacatg
     2461 aatggtgggg caacaaagta accgcgggcg actgggccga ttactggatc catgaaggga
     2521 tcggcacctt tggcgacgcg ctttatacga gggatatgga aggtgaggcc gcgtatgaga
     2581 aatatttcca gcgtatcgcg ccacgcatcc gcaacgacaa gcctgtcgta ctgggtaagg
     2641 atattcccga ggatgaagcc tatcatcccg atatctatcc gaagggagca ttctttatgc
     2701 atacgataag gtatgtcatc ggcgacagca ttttcttttc aacgctgaag cacctggcta
     2761 ccgacagcaa tacgacctat acgcatacca ccagcaccgc ggatgtcgag cggctgttca
     2821 gcggcgcatc ggggatcgat cttgggccgt tgttccatct ctttctgtac accacggaca
     2881 agctggaaat agatgtgcgg cagaccggcg agacgcggta tctagtgcgt ttgcaaaata
     2941 tcgacatgcc gctgccgttg gagatcgtga cggataaggg gagccagcgg gtgatggtcg
     3001 ataaaaaggg cgtcgtcatc aacagccaga gcctgccgct ggtggatccc cgtgtctttt
     3061 acctcaaaaa agtgatctat gaataaagct atgttcgata agactgttta cagggcccgg
     3121 agggcagtac tgaaagcgac ggtgggcaag ggtatgatcc tgctgttggg aaatgaagag
     3181 agcagcatga actacaggga taatctttat ccttttcgcc aggatagcag cttcctgtat
     3241 tatttcgggt tggacaaacc gggtttgacg ggactgatcg atatcgacaa tgaccgggag
     3301 ttcatttacg gcagggaact gacgatggat gagatcgtct ggacgggtcc gcagcctacg
     3361 ctggctgagc aggcggaagc ggcgggtatc ggtgagacgc gggcggtgac cacgttgtct
     3421 tcggacatcg ccagcgcacg gggcgcgggg cgtacgatcc attttctgcc gccttaccgt
     3481 cccgagaata tttcgaaatt gagcggttgg ctgggcattc ccggcggcgg tgtcgcgtcg
     3541 catgcgtcgg tgccgttgat caaagtggtg atcggtcagc gatcggtcaa gaccccggag
     3601 gagatcgagc agatagacca ggctgtcgat acgacggccg agatgcatct ggctgccatc
     3661 gaaggctgcc gggacgggat gaccgaagcg cagctggccg gcagattgca ggcgatcgcg
     3721 atcagcgcgg ggggaaacct ggcttttccg accatcctga cggtcaacgg gcaggtattg
     3781 cataatcact atggccctac cgtgatgcgc gaaggccgtc ttgccatttg cgacgccggc
     3841 gccgagaatg cgatgcatta tgcgggggat atgacgagga ccttcccggt cgggaagaag
     3901 tttaccccgc ggcagcgcga gatgtatgat atagtgttga aggcgcagga gagcgcggcg
     3961 caggcgcttc gtcccggagt tcttttccgg gatgtacatg cgctggcggc aagggtattg
     4021 gtggaaggcc tgaaagacgc gggagtgatg aaaggcgatc cggccgaagc cgtcgcggcg
     4081 ggggttcata cgatggtctt ccaatgcgga ctggggcata tgatggggtt ggacgtgcat
     4141 gacatggagg accttggcga ggaatatgtc ggttacacgg aaacgctgaa gaagagtaag
     4201 gaattcgggt ggaaatcgct acggctggcg cgcgccctcg agccgggatt cgtcgtgact
     4261 gtcgagccgg gtc
//
LOCUS       DASYSV010000200         6415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_674184, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000200 DASYSV010000000
VERSION     DASYSV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674184"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VGQ51_06125"
     CDS             <1..215
                     /locus_tag="VGQ51_06125"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV9036174.1"
                     /translation="CRVQTVRKEINPKLWGLLEAFGRLTGDGMLINTSFNVRDEPIVC
                     SPTEAYMCFRNTEMDLLVIGNIIIFK"
     gene            212..364
                     /locus_tag="VGQ51_06130"
     CDS             212..364
                     /locus_tag="VGQ51_06130"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEV9036175.1"
                     /translation="MMEFLKDLWLFLRERKKWWLAPLIIVIVLIALFIVLGGSSALAP
                     FIYSLF"
     gene            366..734
                     /locus_tag="VGQ51_06135"
     CDS             366..734
                     /locus_tag="VGQ51_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036176.1"
                     /translation="MKFNHDRAKETVAVIAVGFLLLYLIFHRWGFFYVGLGVQVAGVI
                     SSRLSAWIDWLWTGLSKVLGAVSNTVLLSAVFIFVVTPVGLVRRWRGKDRMLKFDRKA
                     TSNFMDRDHVFAGKDLDNTW"
     gene            746..2368
                     /locus_tag="VGQ51_06140"
     CDS             746..2368
                     /locus_tag="VGQ51_06140"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEV9036177.1"
                     /translation="MKKLYLLLTLMVVTAGLLLLVGPGKRMILRSEASGMSFDEDEKE
                     DGAGQALKQEVLMTRDPNLGYVPTERLLRYHDFMRSHPGLRAGGAGSRAVITDGGSGN
                     AVVLGSSDGLTWAERGPNNIGGRCRAVLIDQSDATGNTVLVGSVSGGLWRTTNFTAAS
                     PTWSQNSTVLANLAITTLAQDPTDHSILYAGTGEGYFNIDAIRGLGIYKSTDGGITWS
                     LLSSTTMGGANVNDFNFVQKVAVYSNGDVYAAGISATFCNTGGILKSTNGGSTWSRVT
                     GTVPGSCGACGCVQAYNGYDITFSSGGDIYATVTDGDGTGRILKSPAGASVGNSGSWT
                     DVTPSTGTGLLWQRISLAASPSNNSRVYAFIQDSATGGVGGIRRTDDGGTTWTNVNSS
                     ATWCDGGSSNGTDFARGQAWYNLSIAVNPGNDATVYAGGVDMMKTTNSGANWAQVTQW
                     RSGCSSLPYVHADIHTISFMPGSTTSFIVGCDGGIFYTTDGGSSWVSKDAGLNVTQYY
                     GVALHPSSGSNICWEARRITVRISSAVRGSIR"
     gene            2305..3804
                     /locus_tag="VGQ51_06145"
     CDS             2305..3804
                     /locus_tag="VGQ51_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9036178.1"
                     /translation="MLGGAQDNGSHIFSSAGINSVSSASSGDGVMCFIDQSAPSTQVT
                     SASFANYFISRDGGNTFSLGFTSSDGRFINPTSYDNSAKKLYCGSKDRKLKRIDNIAS
                     GTVAGTDITVIAGITALSVSAVKVDPNTADNVWAAFSTADDASAALAPRLYLLTGASG
                     ASPVITQMGAATLSALPAGSYINSIDVEQGNANHLVMTVSNYGVASVWESTDHGTTWA
                     SLDNNGVNLPDMPVYWAMIVPGTDNVNTGGVNGGIMLATEMGVWSTSSSAGTSTVWTE
                     NSGTMGNVSTRMLQYRSSDNVVVAATHGRGLFTTSITNVPLPLTFTSFSGEARGTGNH
                     LDWVVANEYNNKGFTIERSYGGSAGFTAIGFVAAVSASFHASAGYSFNDSLVDLGRGG
                     AFYRLLQTDLDGKATYSTTISIGRTVSNKLVQYLSASGNMLFLRINSGGDVSALNMRI
                     MDMSGRLVVARDIPDQSQSVDISALARGIYIVKLFGKDGQQFTGRFQKF"
     gene            complement(3801..4988)
                     /locus_tag="VGQ51_06150"
     CDS             complement(3801..4988)
                     /locus_tag="VGQ51_06150"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV9036179.1"
                     /translation="MLSSSSPVIRFSGLDHLRALAIIIVFFYHYGMFGNPPGLRETLG
                     SWGWTGVDLFFVLSGFLIGGQLFASVARNQTISYGEFYFKRSLRILPAFFAVLLLYFT
                     IPAFTERSTLPPFWRFLTFTQNFGLDLKQHGAFSHAWSLCIEEQFYLILPLFLIVFKT
                     NRRSIWLLPILFLAGLAIRSFIWYHILASPDQAAFGTNYYALIYYPTWSRLDGLLAGI
                     ALAALYYFRPATWQRVTRRGNWLLSIALLLIACAWLISHGNNQYGMQGAIFGYPGISI
                     AYGALVLAALSPTCFLYRFSSAITRWIATLSYSIYLTHKQLIHLTHRFLHPLGIGEDS
                     YAALFISIAVAALGGWLLHLAVEKPFLRLRDRILARRRNTATPAVQPTAPPRSAASEA
                     QRH"
     gene            complement(5007..6302)
                     /gene="thrC"
                     /locus_tag="VGQ51_06155"
     CDS             complement(5007..6302)
                     /gene="thrC"
                     /locus_tag="VGQ51_06155"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV9036180.1"
                     /translation="MNYYSLNNISPITSFKEATIRGQAPDKGLYFPETIPAVPADLLK
                     NIDSLSRAELAFRVIRPYVGSTIPDADLHRIVTETIDFDFPLVPISPDIRSLELFHGP
                     TLAFKDVGARFMSRCLGYFVRDTDQPVTVLVATSGDTGGAVANGFHNVEGVNVVILYP
                     SKKVSSVQELQLTTLGKNITALEVNGTFDDCQNMVKQAFADQTLQQALFLTSANSINV
                     ARWLPQQFYYFFAWQQWPDKSNPPVISVPSGNFGNICAGLLAHRSGLPVKHFIAACNA
                     NDVVPQYLQTGNYKPAAARPTLSNAMDVGNPSNFVRVLELFHNQLPEIKNTLSAVSIS
                     DKETTAAIRELYTNHHYLADPHGAVGWLALRRYLDHHPGEKGFLLETAHPVKFYDTVE
                     PIIGEKIPLPATVKSLLNQKKQSTRLDPVYAQLKNFLLS"
BASE COUNT         1401 a         1375 c         2146 g         1493 t
ORIGIN      
        1 cgtgtcgtgt gcaaacggtg cggaaggaga tcaatcctaa actgtggggg ctgctggagg
       61 cattcggcag attgacgggg gatgggatgc tgatcaatac cagctttaat gtcagggatg
      121 agccgatcgt ctgcagcccg acggaggcgt atatgtgttt caggaatacg gagatggact
      181 tattggtcat agggaatatc attatcttta aatgatggaa ttcttaaagg acctctggct
      241 tttcctgaga gaaagaaaga aatggtggtt ggcgccgctg atcatcgtga tcgtgctgat
      301 cgctttattc atcgtgctgg gcggttcgtc ggcgctggca ccttttatat actcgctttt
      361 ttaatatgaa attcaaccac gatagggcta aggagaccgt agcggtgatc gctgtgggtt
      421 ttttgttgtt gtacctgatc tttcataggt ggggtttctt ttatgttggg ttgggggtgc
      481 aagtggccgg ggtgatctcg tcgaggctca gcgcgtggat cgattggctg tggaccgggt
      541 tgtcgaaggt gttgggcgcg gtgagcaaca cggtgttgct ttcggcggtt tttatctttg
      601 tcgtgacgcc ggttgggttg gtcaggcgat ggagggggaa ggacaggatg ttgaaatttg
      661 accggaaggc gacgagtaat tttatggacc gggatcatgt gttcgccggg aaggatctgg
      721 ataatacatg gtaaatggct tgcttatgaa gaaactatac ctgctcttaa ccctgatggt
      781 tgtaaccgcc ggattgcttc tcctcgtagg gccgggaaag agaatgatcc tgcgttccga
      841 ggccagcggg atgtcatttg acgaagacga aaaggaagac ggcgcaggcc aggccctgaa
      901 gcaggaggtc ctcatgacca gggatcccaa tttaggatat gttcccactg agagactgtt
      961 gcgatatcat gatttcatga gaagtcatcc cgggcttcgt gccggcggtg caggcagtcg
     1021 tgccgttatt acggatggcg gttccggtaa cgctgttgtg ttgggctcca gtgacgggct
     1081 tacctgggcg gaaagagggc cgaacaatat cggcggtcgt tgcagggccg tcctgatcga
     1141 tcaatcggat gccacgggga ataccgtttt ggtcggaagc gtaagcggag gtctatggcg
     1201 aacaaccaat tttacggctg ccagtcctac gtggtctcag aacagcacgg tattggccaa
     1261 tttggccatc acgactcttg cgcaggatcc taccgatcat agtatcctgt atgccggaac
     1321 gggggaagga tatttcaata tcgacgcgat caggggactg ggcatttaca agagcacaga
     1381 tggagggata acctggagcc tgctgtcttc tactacgatg gggggcgcca atgtaaatga
     1441 tttcaatttt gtccagaagg tggcggtata ctccaacgga gacgtttatg ccgcagggat
     1501 cagcgctact ttttgtaata ccgggggtat cctgaagtcg accaatggcg gcagcacgtg
     1561 gtcgcgggtg acgggcaccg ttcccggaag ctgtggggcc tgcggttgtg ttcaagccta
     1621 taacggttac gatattacat tttcttccgg gggcgacatt tatgcaaccg tgaccgatgg
     1681 cgacgggacc gggaggatac ttaaatctcc cgcgggcgca tccgttggaa acagcggaag
     1741 ctggacggat gtcactcctt cgaccggaac gggcctgctt tggcagcgta tttcgctggc
     1801 cgcctcgcca tccaacaaca gcagggttta tgcttttata caggattcgg ccaccggcgg
     1861 agtcggtggc atccggcgta ccgatgacgg agggacaacc tggaccaatg tcaatagcag
     1921 cgccacctgg tgcgacggag gtagttcaaa tggaacggac ttcgccaggg ggcaggcctg
     1981 gtataatctc tccattgcgg tcaacccagg aaatgacgct actgtttatg cgggcggcgt
     2041 cgatatgatg aaaacgacga atagtggcgc taactgggcg caggtgacgc aatggcgatc
     2101 ggggtgttct tcgctgcctt atgtccatgc cgacatccat actattagtt ttatgcccgg
     2161 gtccaccact tcttttattg tcggttgtga cggggggatc ttttatacta cggatggcgg
     2221 aagcagttgg gtttcgaagg atgccgggct gaatgtgacg cagtactacg gcgttgcgct
     2281 gcatccttcc agcggcagta atatatgctg ggaggcgcgc aggataacgg ttcgcatatc
     2341 ttcagcagtg cggggatcaa ttcggtgagt tctgccagtt ccggggacgg tgtaatgtgt
     2401 ttcatcgacc aatcggctcc ttccacgcag gtgaccagcg cgagctttgc caactatttt
     2461 atctcgcgtg acggggggaa tacgttttcg cttggtttca cttcttccga cgggcgtttc
     2521 atcaatccga cgagctatga taattccgcg aagaagctgt attgcggaag taaggacagg
     2581 aagttaaaga ggatcgataa tatagccagc ggaacggtag caggaacgga tatcaccgtc
     2641 atcgcgggca ttaccgcatt gtcagtttct gccgtcaaag tggatccaaa taccgccgat
     2701 aatgtatggg ctgccttttc gaccgcagat gatgcgagtg cggccctggc gcctcgtttg
     2761 tacctgttga cgggcgcttc cggagcctcc ccggtgatca cacaaatggg cgcggccacg
     2821 ctttccgcgc tgccggcagg atcgtatatc aacagcatcg acgtggagca gggtaatgcc
     2881 aatcacctgg taatgacggt cagcaattac ggtgtagcca gtgtatggga atccacggat
     2941 catgggacta cctgggcttc gctggacaat aatggggtca atctgccgga tatgccggtg
     3001 tactgggcga tgatcgtgcc ggggaccgac aatgtaaata cggggggcgt caatggcggg
     3061 atcatgctcg ccaccgagat gggggtctgg tcgacctcgt cgtctgccgg aaccagcacc
     3121 gtatggacgg agaattccgg taccatgggg aatgtcagca cgcggatgct gcaatatcgt
     3181 tcttccgata acgtggtggt agcggctacc catggtcggg gtttgttcac cacgtcgatc
     3241 acgaacgttc ctttaccgtt gacttttaca tccttcagcg gcgaggcaag aggaacgggc
     3301 aatcatttgg attgggtggt tgcaaacgag tataacaata agggttttac gatcgaacgg
     3361 agctatgggg gaagtgccgg ctttactgca atcggcttcg tggctgcggt ttcggcgtca
     3421 tttcatgcct cggccggcta ttcctttaac gactcgcttg tggatttggg acgcggtggt
     3481 gctttttatc gcctattgca gacggatctc gatgggaaag ccacctattc aaccaccatc
     3541 agcatcgggc ggacggtttc caataagctg gtacaatatc tttccgcttc cggcaatatg
     3601 ctgtttttgc ggattaatag cggcggcgac gtttcggctc ttaacatgag gatcatggat
     3661 atgagcggca ggctggtggt ggcaagggat attcctgatc agtcacagtc agtggacatc
     3721 agcgctctcg cgcgggggat ctatattgtt aagttgttcg gcaaggatgg gcaacaattt
     3781 acaggacgtt ttcagaaatt ttaatggcgt tgtgcttcgg atgccgcaga ccgggggggt
     3841 gccgtaggtt ggacggcggg tgtcgcggtg ttgcgccggc gtgcgaggat gcgatcgcgc
     3901 aggcggagga acggcttttc aacggcgagg tgcaggagcc atccaccgag tgctgcgacg
     3961 gcgatgctga tgaataaagc ggcatagctg tcttcgccga tgccgagagg atggaggaac
     4021 cggtgagtga ggtggatcag ctgtttgtgg gtgaggtaga tggaatagga tagggtggct
     4081 atccagcggg tgatggcaga agaaaaacgg tagaggaaac acgtcgggct tagcgcggcg
     4141 aggacgagag cgccataggc gatagagatg cccggatagc cgaagatggc tccctgcatg
     4201 ccatattggt tattgccgtg agagatcaac caggcgcagg cgatgaggag gagggcgata
     4261 ctcaggagcc agttgcctct tcgcgttact cgttgccagg tggcggggcg aaaataatag
     4321 agtgcggcaa gggcgatgcc ggcgagcagg ccgtcgaggc gcgaccaagt gggataataa
     4381 atgagcgcat agtagttggt cccgaaggca gcctggtcgg gtgatgccag gatatggtac
     4441 cagatgaagc tgcggatggc gagacctgcc agaaagagta ttgggaggag ccagatggag
     4501 cgccggtttg ttttgaagac gatgaggaag aggggcagga tgagatagaa ttgttcttcg
     4561 atgcatagcg accaagcgtg ggaaaaggca ccgtgttgtt tcagatcgag accgaagttc
     4621 tgggtgaagg taaggaagcg ccagaaggga gggagggtac tccgctccgt gaacgcgggg
     4681 atggtgaaat agaggagcag gacggcgaag aatgcgggga ggatgcggag tgaacgtttg
     4741 aaatagaatt cgccgtagga gatggtttgg ttgcgggcga cggatgcgaa aagctggcct
     4801 ccgatgagaa aaccgctgag gacgaaaaaa aggtcgacgc cagtccagcc ccagctaccc
     4861 agcgtctcgc gcagaccggg cgggttaccg aacatcccgt agtgataaaa gaacacgata
     4921 atgatggcga gtgcccggag gtgatccaga ccggagaatc ttatgacggg tgatgaagaa
     4981 ctaagcaagt gctgtcgagt tggggatcaa gatagcaaaa aattcttcaa ctgcgcgtat
     5041 acgggatcga ggcgggtgct ttgttttttt tgatttaaca atgattttac tgtcgccgga
     5101 agagggatct tttcaccgat gatcggttcg accgtatcat agaattttac ggggtgagcc
     5161 gtttcgagga ggaagccttt ttcgccgggg tggtggtcga ggtagcgtcg tagtgcgagc
     5221 caaccgacag cgccgtgcgg atcggcgaga tagtgatggt ttgtgtagag ttcgcggatg
     5281 gcggcggtgg tttctttgtc ggagatgctg acggcggaga gggtattttt tatttcgggg
     5341 agttggttat ggaagagttc gaggacgcgg acgaaattgc tgggattgcc gacgtccatg
     5401 gcgtttgaga gcgtggggcg ggcggcggcg ggtttataat tgccggtttg cagatattgg
     5461 ggaacgacgt cattggcatt gcaggccgcg atgaaatgtt ttacgggaag gccggaccga
     5521 tgggccagga ggccggcgca gatgttgccg aaattgccgc tggggacgct gatgacggga
     5581 gggttggact tatcgggcca ttgctgccag gcgaagaaat aatagaattg ctggggcagc
     5641 cagcgtgcga cgttgatgga attggcggaa gtaaggaaga gcgcttgttg gagggtctgg
     5701 tccgcgaaag cctgttttac catgttctgg cagtcgtcga aggtgccgtt gacctcgagg
     5761 gccgtgatat tctttcccag ggtggtgagc tggagttcct ggacgctgct gacttttttt
     5821 gagggataga ggatgacgac gttgacgcct tcgacattgt ggaaaccatt ggctactgcg
     5881 ccgcctgtat cgcccgaagt ggcgacgagg acggtgacgg gttggtcggt gtcacggacg
     5941 aaatagccga gacagcggct catgaagcgg gcgccgacgt ctttgaaggc gagggtggga
     6001 ccgtgaaaga gttcgagtga ccggatgtcg ggcgagatgg gaacgagggg gaagtcgaaa
     6061 tcgatggttt cggtaacgat gcgatgcaga tcggcatcgg ggatagtgct cccgacatag
     6121 ggacgaatca cccggaaggc gagctcggcg cgggagaggg agtcgatatt ttttagcagg
     6181 tctgcgggga cagcggggat ggtctccggg aaatagaggc ctttatcggg cgcctggccg
     6241 cggatggttg cttctttgaa actggtgatg ggtgaaatat tgttaaggct atagtagttc
     6301 ataagatctt tactccctgt tggttgactg tggtgatata ggtgtggtgg tccaggccga
     6361 tgcgggtata gatatcgcgc atacaggatt cgacctggcg ggccgtttgc tgatc
//
LOCUS       DASYSV010000201         6932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_37301, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000201 DASYSV010000000
VERSION     DASYSV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37301"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VGQ51_06160"
     CDS             <1..830
                     /locus_tag="VGQ51_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9036181.1"
                     /translation="RLTVAYDFLKVMGITPVSGRDFSTAYPSDTATDVENVVINQSMA
                     SQLGIRNATGLSFFPDSAEPKWNVIGIVPDFHLYSLYESAGPMTISMVRPGRPLNYIL
                     VKVRTDNPSTAMRFVQTAFRKLEPDNPVNGSFLTENVQRWYEKEQRLADIFFSAAVIA
                     ILLSCLGLFAIVALIMEQRRKEIGIRKVLGASMPSVAGMLSKEFLGLVGVAFLIAVPI
                     SWYFLHRWLDNFLYKTSIAWWIFPLAGAAVVGIAMITVGLQTLRAAAVSPVDALRSE"
     gene            complement(901..1137)
                     /locus_tag="VGQ51_06165"
     CDS             complement(901..1137)
                     /locus_tag="VGQ51_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036182.1"
                     /translation="MRNLLVFLLISGLMAGACTSRNRVGPEHSDGNGGVLAPDSTTRY
                     DTSHPREHSDTSGQLHDTIATPDTERASNPHRHP"
     gene            1254..2633
                     /locus_tag="VGQ51_06170"
     CDS             1254..2633
                     /locus_tag="VGQ51_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036183.1"
                     /translation="MHTAKKDRIRLPFEVDTQTWKDIDLFDKGGVGPAFTLFCRVKTA
                     GGAEALERMLKNPSNHRALLEGRRDIIRFFCDAGVDLPIGREQLKKVESYLSSVFPRL
                     PDSRPKILLFSIRNQLQKQPVYFAIIQGIRTTVSLLRHLFQLRESWLDRDCPAMLMDE
                     LEGLPESITDRRVRELILGESRHLTPVDLACCDRYFRGSGKVFLRRLLQMLYDWDVFE
                     AVAMVVQQYDLCFPQYNDAPDPVVEVQGLFSPLLAGGVCNDLELGGDTNLCFITGAGM
                     AGKSTFLKSFGLAVYLAHIGFPVPAHSFRTTIFNGLITTVDLGDSIGQGYSRCYAEVN
                     RIKETLRQIKEKRRVLVIFDELFRGTPAKDAGGALLQVADALAGIGDSLFLVSTSIGE
                     IADGLQGNPNIRFACFESTMEGGVPWYSYRLREGVSNERMGMLLLRNEGVLEMLEGMQ
                     TSRISRDAG"
     gene            2762..3244
                     /locus_tag="VGQ51_06175"
     CDS             2762..3244
                     /locus_tag="VGQ51_06175"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEV9036184.1"
                     /translation="MEKMPVSSYTVRFNDCDPMGHLNNARYIDYFLNAREDHLRDHYD
                     IDLREWAKQGIGFVVSQHEIRYLRPVNYNDKVVIRSALIGWGETTLLVEMLMTGVAGQ
                     LKAIMWTEFTRIDPRTGKRQAHPEDFMAWIEGTLVGGIDLSNGLNGRIEALREQVARG
                     "
     gene            complement(3307..3379)
                     /locus_tag="VGQ51_06180"
     tRNA            complement(3307..3379)
                     /locus_tag="VGQ51_06180"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3344..3346),aa:Lys,seq:ctt)
     gene            complement(3439..3573)
                     /locus_tag="VGQ51_06185"
     CDS             complement(3439..3573)
                     /locus_tag="VGQ51_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036185.1"
                     /translation="MPIAFRIGLLHSFVGGDAALLPHQDSPASTSEWLVSVIGIPQKP
                     "
     gene            3965..4648
                     /locus_tag="VGQ51_06190"
     CDS             3965..4648
                     /locus_tag="VGQ51_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9036186.1"
                     /translation="MLSRPISLAIVDDHALFRKTLKNFLCEQQNIHVAVEVPDVFTLF
                     NRLKTTPIDVLLMDIFLPGMNGDEALMNIRLQYPDVKILMLSMCTDMDLICDLLEKGI
                     HGYISKGDEPEDLLQAIRTVSENRIFRNRLFTEALYWNKQNTFKSVAERNHVLLNERE
                     KKILQLMWEEKSNKEIADALFLGVRSVEKIRQDLKEKVGVKSTVGLLKYAINKRIIGA
                     SVQKPDLIK"
     gene            4901..5413
                     /locus_tag="VGQ51_06195"
     CDS             4901..5413
                     /locus_tag="VGQ51_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HEV9036187.1"
                     /translation="MNNFRSGWYLIYTKPRHEKKVHERLSQLDIRSFLPITRKLRSWH
                     DRKKYVYEPLFPSYVFVYLKDMQHYYYGMDTEGALYYVRTGKDIAKVNESIVDSIRLL
                     TESGTGIEVSAGNIAPGQQLTIHQGPLTGVSGEVVQVRGSQKIVVRVHLLQRNLLAIL
                     PSDYLMPVHS"
     gene            5424..>6932
                     /locus_tag="VGQ51_06200"
     CDS             5424..>6932
                     /locus_tag="VGQ51_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEV9036188.1"
                     /translation="MEELVYRLKMSGIGIAVVGDQIKLDIPEGKDVSALVSEVRHHKQ
                     ALLLYVRKSQPDPLSRTPAPRLVEKKEHYEVFHQQKKEYLRYLILGDHSFNSNFILSS
                     DHPDLPTLHRALRTLFFRHESLRTTFLRKDGQLRQVIHGIDTVTLNVEYLDLSAEGNK
                     QELADRLLEQSAGRRFDFEKELLSDIKLVRYLPDAYYLLFTTHHAICDAFSSAILKRE
                     IGLLYQAYLQGDDDPLPPLTFQYKDYAFWANRCLKGRYGREAQTFYKGTIGRSLAEEV
                     AVAGGVQQWSSGYRQRLREELIRVQGPTGPARFPEAFGAVVNLLPEKGGFYKTYIEGD
                     VIGRLKRTTLEKGATFFSVLLTAFALLLSRETKVRTARISVPHTTRLFEEFENIVGWL
                     TSEIIVCIPVDPSMHLDRMLEEVAAIIQAASDHCIYPHEKILDDMDIPLEVMAPALLN
                     YIGDPDAGMPRFQPYHTDNGSGHFNLHCKVTEYSDGMTVEMNYNVHVYARSRV"
BASE COUNT         1645 a         1677 c         1936 g         1674 t
ORIGIN      
        1 cgcggctgac ggtggcttat gattttctga aagtgatggg cataacaccc gtgtcgggcc
       61 gggacttcag caccgcttat ccctcggata ccgctacgga tgtggaaaat gtcgtcatca
      121 atcaaagcat ggccagtcag ctggggataa ggaatgcaac cggtctgtcc ttttttccgg
      181 atagcgccga accgaagtgg aatgtcatcg gcatcgtccc ggacttccat ctttattcgc
      241 tgtatgaatc ggcgggaccg atgaccatct cgatggtcag gccggggaga ccgctgaatt
      301 atatcctggt gaaagttcgc accgacaacc cttccacagc catgcgtttc gtccagaccg
      361 cctttcgtaa actggagccg gacaaccccg tcaacgggtc cttcctcacc gagaacgtcc
      421 agcgatggta tgagaaggaa cagcggctgg cggatatctt tttttcggcg gccgtcatcg
      481 ccatcctgtt atcctgtctg gggttgttcg caatcgttgc gttgatcatg gaacaacggc
      541 gtaaagagat cggcatccgg aaggtgttgg gggcttccat gccgtcggta gcggggatgt
      601 tgtcgaaaga attcctcggt ctcgtggggg tggcattcct gatcgccgtg cccatttcct
      661 ggtatttcct gcatcgctgg ctggacaatt tcctgtataa gacctctatc gcctggtgga
      721 tcttcccgct ggccggcgcg gcggtcgtcg ggatcgcgat gatcacggtc ggtctgcaaa
      781 cgttgcgggc tgccgcggtc agcccggtgg atgccttgcg tagcgagtga atgccggtca
      841 gtcctgtcga tgccctgcga gtgaatgccg gccggccccg cgatgccctg cgagtgaatg
      901 ctacgggtgt cgatgtggat tacttgcccg ctccgtatcg ggagtggcga tcgtatcatg
      961 cagctggccg ctggtatcgg agtgctccct cggatggctg gtatcgtaac gggtggtgct
     1021 gtccggtgcc agaacgcccc catttccgtc gctgtgttcc ggcccgaccc ggttcctgct
     1081 cgtgcaagcc cctgccatta accccgagat cagtaaaaaa actaataagt tacgcattgt
     1141 aatccattta ggtgatcgat ccaatgcaag tattcaaatc tcctgccacg gtaattccga
     1201 tttatttaac aaagaagatg caacatcggt gaacccttgc acgtcctatg gtcatgcaca
     1261 ccgctaagaa ggaccggatc cggcttccct tcgaggtaga cacacagacc tggaaggata
     1321 tcgatctttt cgacaagggt ggggtagggc ccgcatttac tcttttttgc cgggtaaaga
     1381 ccgcgggtgg agccgaggcg ctggagcgga tgttgaagaa cccgtcgaat catcgggcgt
     1441 tgctggaagg gcggcgggac atcatccggt tcttctgcga cgcgggtgtc gatctgccta
     1501 tcggcaggga acagctgaag aaggtggaat cctatctttc gtcggtcttt ccccgtctcc
     1561 ccgattcgcg gccgaagatc ctgctctttt ccatccggaa ccaactgcaa aagcaacctg
     1621 tttatttcgc gatcatccag ggtatccgaa cgaccgtcag tctgctgcgt catttgttcc
     1681 agctgcgtga gagctggctg gacagggatt gtcccgcgat gttgatggac gagctggaag
     1741 gactgcccga atcgatcacc gatcgaaggg tccgggaatt aatacttggc gagagcagac
     1801 atctgacgcc ggtggacctg gcttgttgtg accggtattt ccggggcagc ggaaaggttt
     1861 ttctgagacg gctgctgcaa atgctgtacg actgggatgt gttcgaggcc gtggcgatgg
     1921 tagtgcaaca atatgatctt tgttttcctc aatataatga cgcaccggac ccggtcgtcg
     1981 aagtacaggg ccttttctct ccattgctgg cgggcggcgt ctgcaacgat ctcgaactgg
     2041 gaggagatac gaatctgtgt tttatcacag gagccgggat ggcgggaaag tcaacatttt
     2101 tgaaatcctt cggattggcc gtttacctgg cgcatatcgg gttcccggtg ccggcccatt
     2161 ctttccggac gacgatcttc aacgggctga tcacgaccgt cgatctgggg gatagtatcg
     2221 gtcaggggta tagccggtgt tatgccgaag tgaacaggat caaagagacc ctccgccaga
     2281 taaaggaaaa gcgaagggtc ctggtcattt tcgatgagct gttccggggg actcctgcaa
     2341 aagatgccgg cggcgccttg ctgcaggtgg cggacgctct tgcgggcatc ggggatagcc
     2401 tgttcctcgt ctccacttct atcggagaga tcgcagatgg cctgcagggg aatcctaaca
     2461 tccgttttgc ctgtttcgag tctacgatgg aaggcggcgt accctggtac tcctaccggc
     2521 tgcgcgaagg ggttagtaat gaaaggatgg gtatgctgtt gctccgcaat gagggagtgc
     2581 tggagatgct cgagggcatg cagaccagcc ggataagccg ggatgcggga taggcgcggt
     2641 gccagccgcg atgcgggacc tgcgcgatgc ccgccgcgaa gcgggcgagg gtggcgccat
     2701 aggtttggac cctgagattt tagttttact tttgggtacc aaattcaatc aattctattt
     2761 tatggaaaag atgcctgtct cttcgtatac ggtccggttc aacgactgcg atccgatggg
     2821 tcatttgaat aatgcccggt atatcgatta ttttttgaat gcccgggagg atcatttgag
     2881 ggaccattat gacatcgatc tccgggaatg ggcgaaacag gggatcggct ttgtggtgag
     2941 tcagcatgag atccggtatt tgcggccggt gaattacaat gacaaagtgg tgatccggtc
     3001 ggcgctgatc ggatggggcg agacgacgct gcttgtggaa atgctgatga cgggtgtcgc
     3061 gggtcagctg aaggcgatca tgtggacgga atttacaagg atagatcccc gtacgggaaa
     3121 acggcaggcg catccggagg attttatggc atggatagaa gggacgctgg tcgggggtat
     3181 cgatctttcc aatgggttaa acggtcgcat tgaagcatta cgggagcagg tcgcgcgagg
     3241 ataataagaa tctttttatg aaatagaaaa agcaccggac gatgtcgtca ggtgcttgat
     3301 tttcagtgtc cccgcacggg ctcgaaccgt ggacccgctg attaagagtc agctgctcta
     3361 ccaactgagc tacagagaca tctgtctttt gacgggggtg caaagataag aattgtaccg
     3421 gtttcaaaaa gaatattttt agggtttttg gggaatgccg atcaccgata ccagccactc
     3481 cgatgtggag gcgggactgt cctggtgtgg aagtaacgcg gcatctcctc ccacaaagga
     3541 atgaaggagc ccgatccgga aagcgatcgg caagatcttt tgatcgtaga aacgatttac
     3601 tgcaaaagga tcgcggtcaa ctagagtatt ttcaagtcgc attccgaaaa aaaatccacc
     3661 tgctttcaga caaaagtcgc cgtggttgcc gcgaccgggg atacatgaaa gcgtatgcgc
     3721 ctaaccgttt tattaaaagc agtttttaac aagagaaaat caatagctgt gggcgaaaat
     3781 gcccatggga tgtagtacct tgtcaggact ttaggatccc gtaccatccg tagcccgata
     3841 ggcaaggtta aggttaagat aaccacgagc gatcatgctt agaaatagct tggcacagtg
     3901 tgtgcggtaa gcatagatca atttgaacat cgctggtaat gtttgaactt acaactttaa
     3961 ccttatgcta tctagaccca tcagccttgc tattgtcgac gaccacgctc tattccggaa
     4021 gacgctcaaa aattttctat gcgaacaaca gaacattcat gttgccgtgg aggttccgga
     4081 cgtattcact ttgttcaata gattaaaaac cactccgatc gacgtattgt tgatggatat
     4141 ttttttgcca ggcatgaatg gggatgaggc attgatgaac attcgcctgc aatatcccga
     4201 tgtcaagata ctgatgttgt cgatgtgcac cgacatggac ctgatctgcg atctgctcga
     4261 aaaagggatc catggctata tttcaaaggg ggatgagccg gaagatctgc tccaggctat
     4321 ccggacggtg tcggagaaca ggatcttccg caaccggcta ttcacagagg cgctttactg
     4381 gaataaacaa aatacgttca aatcggtggc ggaaagaaac catgtcctcc tgaatgaacg
     4441 ggagaaaaag atcctgcaac tgatgtggga ggagaaaagc aataaagaga tagccgacgc
     4501 gctctttctc ggcgtgaggt ctgtcgaaaa gatacgacag gatcttaaag aaaaggtcgg
     4561 cgtaaagtcg acggtaggtc tgttgaagta tgccatcaac aaacgcatca tcggcgcgag
     4621 cgtgcaaaaa ccagacctca tcaaatgatg gaatactgca acagtgctgc ttgctttagt
     4681 aataaccgaa aagagaattg gggacactac gaaaggaatg cacctcattt ttgatttata
     4741 tttgttccgt ctaaaataaa tgctctatcc ttaagagacc tcctccccta agactgtaaa
     4801 ttgcccttga tcattgttct tgtttgttta gcaacgagct gtgttactcg ctgtagcggt
     4861 agctgcaacc ttcctattcg aaacctcatc ttcgacacat atgaataatt tccgatcggg
     4921 ctggtacctc atttacacaa aaccacgcca tgaaaagaaa gttcatgagc gactttccca
     4981 gctggacatc cgctcctttc ttccgatcac caggaagctt cgatcctggc acgaccgcaa
     5041 aaaatacgtc tatgagcctt tattcccgtc ttatgtattc gtttatctga aggatatgca
     5101 gcactattat tatggtatgg atacggaagg ggcgttgtat tatgtacgta cgggaaagga
     5161 tattgcaaaa gtgaatgaat ccattgtcga tagtatccgt ttgctgacag aaagtgggac
     5221 cgggatcgag gtttcggccg gaaatatagc cccgggccag caactgacca tccaccaggg
     5281 accacttacg ggagtgtccg gggaggtagt acaggtaaga ggaagccaga agatcgttgt
     5341 gcgtgtccac cttttgcaac gtaacctatt ggccatactg ccctctgatt atttaatgcc
     5401 ggtgcatagc tgattaattt tcgatggaag agctggtata tagactgaag atgtccggta
     5461 taggcattgc cgtggtgggc gaccagatca agctggatat tccggaaggc aaagatgtat
     5521 cggctttggt gtccgaggtg cggcaccata aacaagctct gctcctgtat gttcggaaat
     5581 cgcagccgga ccctctctct cggacgccgg ctccccgcct ggtggagaaa aaggagcatt
     5641 atgaagtttt tcatcagcag aaaaaggaat acctgcggta tctgatcctg ggtgaccatt
     5701 ctttcaattc gaacttcatt ctttcatccg accatcccga tctccccaca ttgcaccggg
     5761 cgctcaggac attattcttc cggcacgaaa gtttgcgcac gacttttttg agaaaggacg
     5821 gccagctccg gcaggtgatc cacggtatcg acacggttac ccttaacgta gaatacctgg
     5881 acctttcggc cgagggtaat aaacaagagc ttgccgatcg gctgctggaa caatccgccg
     5941 gaaggcgttt tgacttcgaa aaggaactct tgtccgatat aaagctcgtt cgttatctgc
     6001 ccgatgccta ttatttgctg ttcaccaccc accatgctat ctgcgatgct ttttccagcg
     6061 cgatcctcaa gcgggagatc ggactgctgt atcaggccta tctgcaaggc gacgacgacc
     6121 cgctgccccc gttgacgttt caatataagg actatgcatt ttgggccaac cgctgtctga
     6181 agggcagata tgggagggaa gcgcaaactt tctacaaggg taccatcggc aggagtctgg
     6241 ctgaagaggt cgccgtcgca ggcggtgtac agcaatggtc ttccgggtac agacaacgtt
     6301 tacgggaaga gttgataagg gtacagggac ccactggtcc ggcccggttc cccgaagcct
     6361 tcggcgccgt ggtcaactta ttgccagaga aaggaggctt ttacaaaacc tatatagaag
     6421 gcgacgtgat aggtcgcctg aagaggacga ccctcgaaaa gggggcgaca ttctttagtg
     6481 tgttgctaac cgcattcgcc ttgctgctga gccgggagac caaagtcagg actgcgagga
     6541 tcagcgtccc gcatactacc cgactttttg aagaatttga aaatatcgtc ggctggctga
     6601 cgtccgagat catcgtctgt atccctgtcg acccatccat gcatcttgac cggatgctgg
     6661 aagaggtagc ggccattata caagcagcgt ccgaccactg catctatccg catgagaaaa
     6721 ttctggacga catggatatt cccctcgagg tgatggcccc cgctctattg aattatattg
     6781 gggatcccga tgccgggatg ccgcgcttcc aaccctatca taccgataac gggtccggac
     6841 atttcaacct gcattgcaaa gtgacggaat acagcgatgg catgaccgtc gagatgaatt
     6901 acaatgtgca tgtctatgca aggagccggg tg
//
LOCUS       DASYSV010000202         3202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1059896, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000202 DASYSV010000000
VERSION     DASYSV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059896"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGQ51_06205"
     CDS             complement(<1..218)
                     /locus_tag="VGQ51_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036189.1"
                     /translation="MKATIVVIGNGMVGHRFCDRLKALGGFRIVVFGEEPRPAYDRVH
                     LSEYFSGKTAAELALPMRGWFEDEEIVL"
     gene            310..1107
                     /gene="cobA"
                     /locus_tag="VGQ51_06210"
     CDS             310..1107
                     /gene="cobA"
                     /locus_tag="VGQ51_06210"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEV9036190.1"
                     /translation="MKSTPPGTELILMGAGPGDPELLTVKAWRILQNADILLYDRLIN
                     PALLDLTPAGCERICVGKRPEGPSTSQETIHRLIREKANKGGTIVRLKGGDPYIFGRG
                     FEEAQVAHSLGLKVTYIPGITSMQTTGLAEIPLTHRSVSEGIWILTGTRKDGSLPEDL
                     RLAIHSRSTVVIYMGMKKLAEIAAVYLQKGKENTPAAIIRNGSLRNQKMVLGTAGSLP
                     AMAAAQGLSNPAILIIGEVVRFSSSRIWRRPGIWQDQCPLTDASTTP"
     gene            complement(1031..1684)
                     /gene="fsa"
                     /locus_tag="VGQ51_06215"
     CDS             complement(1031..1684)
                     /gene="fsa"
                     /locus_tag="VGQ51_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV9036191.1"
                     /translation="MKFFIDTADLAQIKEAHDLGILDGVTTNPSLMAKVGVKGEAAVT
                     NHYKTIAELVDGDVSAEVVSTEFNGIIEEGKKLHAIHPNIVVKVPMIKDGVKALKWFA
                     DNGIKTNCTLCFSAGQAILAAKAGANYISPFIGRIDDSNWDGLLLIEQIAEIYAVQGY
                     ETEILAASIRSPLHIVKCAEAGANVVTSPLESIMGLLKHPLTDIGLAKFLADAKSWS"
     gene            complement(1757..1856)
                     /gene="ffs"
                     /locus_tag="VGQ51_06220"
     ncRNA           complement(1757..1856)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGQ51_06220"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1902..3164)
                     /locus_tag="VGQ51_06225"
     CDS             complement(1902..3164)
                     /locus_tag="VGQ51_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HEV9036192.1"
                     /translation="MKYMVAVLLLLAAGRVGAQVPDLVYSSSIGTPQLFMAGNQAAYP
                     ILRMNSPDQLELHFDDLDGVKNYYYTVVLCNEDWTPAEVSEFDYIKGFSQIRIDNYTL
                     SSVALTHYVHYQAVVPDPNCQLIHSGNYLLKVYVEGDTSKLAFTKRFLVVDSRVPIQS
                     QLLTPLNPELSHTGQNIQFRLNTAQINPANPLDQVKVDVLQNYRWDNVIRGIQPTFAT
                     GNRLEYTNPDAFNFEGGYEWRWIDLESFRFQSARVESVNYGKTATDILAKPDPELSHR
                     EYYFFQDFNGKFIIRTTESYNPLFQADYATVHFSFIPPGGAAFADKDVYMLGRFTGGG
                     LNDSTRMAFNPGTGRYERSFFLKMGYYSYSYVTVDRNDPSMKPSFAETEGNHVETEND
                     YMILVYYRALGSRADELVGMTRFNSLNK"
BASE COUNT          808 a          918 c          747 g          729 t
ORIGIN      
        1 tgaagaacga tctcctcatc ctcgaaccat ccccgcatgg gcaaggccag ttcggcagcc
       61 gtcttcccgc tgaaatactc gctcaaatgg acccggtcgt aagccggacg aggctcctcg
      121 ccaaagacca cgatacggaa accacccagg gctttcagcc ggtcgcaaaa ccggtgtccg
      181 accattccat taccgatgac gactatcgta gctttcatat taaaattata tcaatataat
      241 gttattaggc aatattacgg attattttcg ctaatttcac ataaatactc atataaccat
      301 tattacaata tgaagtcgac cccacccgga actgaactga tcctcatggg cgccgggcct
      361 ggtgatccgg aattgctgac agtaaaagcc tggcgaatcc tccaaaatgc ggatatttta
      421 ttatatgacc ggctcatcaa ccccgccctt cttgatctga cgcccgccgg ctgcgaacgc
      481 atctgcgtcg gcaaacgccc cgaaggacca tccacctccc aggagaccat ccaccgtctt
      541 atccgtgaaa aagccaataa aggcgggacc atcgtccgac tcaaaggagg cgacccttac
      601 attttcggca gaggcttcga agaggcacag gtagcccatt cactcggttt gaaggtcacc
      661 tatataccgg gtatcaccag tatgcaaacc accggtctgg cagagatacc ccttacccac
      721 cggtcggtca gcgaaggcat atggatcctc acggggacga gaaaagatgg cagcctcccg
      781 gaagacctcc gtctcgccat ccatagcagg tcgacggtgg tcatatatat gggcatgaaa
      841 aagctggccg aaatcgcggc cgtctatcta cagaaaggaa aagagaacac tcccgcggcg
      901 atcatccgaa acggaagcct gcgcaatcaa aaaatggtac tgggcacagc gggctctctg
      961 ccggccatgg cagccgctca gggtctctcg aaccccgcca tcctaatcat cggtgaagta
     1021 gtacgcttct ctagctccag gatttggcgt cggccaggaa tttggcaaga ccaatgtccg
     1081 ttaacggatg cttcaacaac cccatgatcg actccagggg cgaagtcacc acattggcgc
     1141 cggcctcggc acatttcacg atatgcaacg ggctgcgaat gctcgcggcc aggatctcgg
     1201 tctcatatcc ctggacagca tagatctcgg cgatctgttc gatcagcagg agaccgtccc
     1261 agttgctgtc atcgatacgc ccgataaacg gagaaatata gttggcgcca gcctttgcgg
     1321 caaggatagc ctgacccgcg gaaaaacaaa gcgtacaatt ggtcttgata ccgttgtccg
     1381 caaaccattt caatgccttt accccatcct tgatcatcgg gacctttacc acgatattcg
     1441 gatgtatggc atgtaatttc tttccttcct cgatgatacc attgaattcg gtcgatacaa
     1501 cctcggcgct gacatccccg tctaccaatt ccgcgatcgt cttatagtga ttggtcactg
     1561 ccgcctcacc cttgacgccg actttggcca ttaaggaagg attcgtggtt acgccatcta
     1621 aaatgcccag gtcgtgtgct tctttgattt gcgccagatc ggccgtgtcg atgaagaatt
     1681 tcatataatg atgctttgaa tttatgtctg cccttcgggc gagccaccgg gaggtggctc
     1741 cgggagggaa ataaaaaatg gcaaagttac ttatatcgca ccgctacgac cacctaccgt
     1801 tgctgccttc cggccctggc ggggttcagc gggagctggt cgtataagac tttgccagtg
     1861 caaatatacg ccattcgcag atattccgcc gcaaaaaaat tttacttatt caaactattg
     1921 aacctcgtca tgcccaccaa ttcatccgcg cgcgaaccca gcgcgcgata atacacgagg
     1981 atcatataat cgttctccgt ctccacatga ttgccttccg tctccgcgaa cgatggtttc
     2041 atcgatggat cattcctgtc cacggtcaca tagctatagc tataataacc catctttaag
     2101 aaaaaactgc gctcataacg acccgtcccg ggattgaatg ccattcgcgt ggaatcattc
     2161 aaccctccac cggtgaacct ccccagcata taaacatcct tgtccgcgaa tgcggctcca
     2221 ccgggcggga taaaactgaa atggaccgta gcataatccg cctggaacag cggattatag
     2281 ctctccgtcg tccggattat gaacttcccg ttgaagtcct ggaaaaaata atattcccga
     2341 tggctcagct ccggatcggg tttggcaagg atatcggtcg cggtctttcc gtaattcacc
     2401 gattcgaccc gcgcgctctg aaaacgaaag ctctccagat cgatccaacg ccattcatac
     2461 cctccttcga aattgaaagc atcgggattg gtgtattcga gccggttgcc cgttgcaaat
     2521 gttggctgta tccccctgat cacattatcc caacggtagt tctgcagcac gtctaccttc
     2581 acctggtcca gcggattggc aggattgatc tgcgccgtgt tcaaccggaa ctggatgttc
     2641 tggcccgtat gcgacagctc gggattcaac ggcgtcagca gctgactctg tatcggcacc
     2701 cgcgagtcca ccaccaggaa ccgcttcgtg aacgccagtt tcgaggtgtc cccttcaacg
     2761 tataccttaa gcaggtagtt tcccgaatgg atcaactgac aattggggtc cggcacgacg
     2821 gcctggtaat gcacataatg cgtcagcgcc accgaggaca acgtataatt atcgatgcgt
     2881 atctgcgaaa aacccttgat atagtcgaat tcgcttactt ccgcgggagt ccagtcctcg
     2941 ttgcataaaa ccaccgtata ataataattc tttacgccgt cgagatcatc aaagtgcaat
     3001 tccagctgat ccggcgaatt catgcggaga atgggatatg cggcctgatt gcctgccatg
     3061 aacagctgcg gcgtcccgat cgaggaagaa tagacgagat cgggcacctg agcaccaaca
     3121 cggcccgcgg ccagcaaaag gagcactgcc accatatact tcatacccat aatattaaca
     3181 caaaaatttc attgctaatg cc
//
LOCUS       DASYSV010000203         4928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_674536, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000203 DASYSV010000000
VERSION     DASYSV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674536"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VGQ51_06230"
     CDS             <1..849
                     /locus_tag="VGQ51_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEV9036193.1"
                     /translation="AIEDRLQTEGAIKFRGVHIDSTETFQRIVDAISNKFLRYIDGNS
                     PRTRLSDHVYTSTEYDQTQRITMHNELSYSAKWPNKLFFTCLQPAVTGGETLLADSRE
                     ILQRMDPDIVREVEQRGITYIRNLHGNTGMGPSWQDTFETKDKRQAEDYCKSYGMQFE
                     WGERDSLRLIQPSRGILRHRATGEKVWFNQIDQFHPSHLGEEMYEVIQLMYGEPENFP
                     MYVRFGDGTAISASLVGEVLKTIDEVTAAPVWERDEFLLIDNELVSHGRNPFSGQRKV
                     LVAMSE"
     gene            862..1632
                     /locus_tag="VGQ51_06235"
     CDS             862..1632
                     /locus_tag="VGQ51_06235"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEV9036194.1"
                     /translation="MDLDKDNVIGIVGGMGPVAGLTLFHRITELTAAAADQEHLSVVM
                     MSFPRHFADRTAFLDGETLVNPAYNVAETIGKLEQAGAKVVGIACNTCHTPAIFDIIL
                     QELHKRRSRIKLVHMLAETCGYIRENHPDVHRVGVMCTNGTYKSGTYGRLLREWGYEA
                     ILPSVDFQEGVIHKMVYDPVTGIKANPTILSPSTHSYMERALRYFEDRQAEAVVLGCT
                     ELSLLQLTRGYTGPLVIIDSTDAMAEGLIRAAKNNHST"
     gene            1629..4709
                     /locus_tag="VGQ51_06240"
     CDS             1629..4709
                     /locus_tag="VGQ51_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HEV9036195.1"
                     /translation="MKTHGVLYRLTLLWLWGACLPYSLFSQERKADSLIRLADREVPA
                     WMRAGNIPGLSLVLIIDGHPYFRNYGFADVEKRTPVTSHTLFELGSCSKAFTALAAAR
                     LIGEGRLHPDDPVSKYIPWWQVSYKDTPVTVTIGQLMHHTSGIPWSTISKIPIISGKD
                     ALEQTVRQLRGQELNRLPGKKFEYATINYDVLALIIQRVAGQPFEQYVQQQVIDPLRL
                     SRTTIGYPIDQARKAEGYKIGFFKARKYDAPVFGGNNAAGYVNTDAVDMAKWVLFQIG
                     LEDSALYPAARYTQQRDESVPLHGMDSYASGWQVALDGTGEIFHEGTNPNFSSYVVFR
                     PAKKTGVAIVTNSNSSYTPVIGNRLIKLLAGEKIEKEFDPGDNFDKVFSVTSLLLGGY
                     ALIVLTFLVLVVLDIIRRRRTYQGYSGSQLRKIGLLLLALLPYAAGLYLAPKAVFGFT
                     WQAMIVWTPASFLLALWLLIFCIGLTCGAYVLSVGFPEKNEYRRMTPKLLLLSILSGL
                     ANMLLIILITSSLDSEVKLGYLVFYYLLMLSVYLMGRMFVQVSLINFTMGVIYEIRMR
                     LTDKIFETSYQKFEKIDRGMVYTTMNDDVGTIGDSTSMFVVLVTSIFTATGALLYLAS
                     IAFWATLLTFFFIASLSTLYYLIIRSMNKYYEQARDTRNVFMRLLNGMIDGYKELSLR
                     RNKKQGYREDIAASADAFRVKMSVASIKFVKTSLVGEAVLIAILGMVAFAFPKVFPGI
                     QAYTLMSFIIVLLYLTGPVNAIVNSVPGILRLRVAIRRIRQFLRDIPSGADKKGGRVL
                     SASATVDSIRAEGIVFRYDQDDSIRPFTVGPVNLEIGRGEIVFIIGGNGSGKTTLAKL
                     FTGLYQPEEGHFLINGEVVESGWLGEYFSAVFSPVHLFEKLYAIDVEGRSEEVNNYLK
                     LLDLDQKVSVRRNAYTSLRLSGGQRKRLGLLQCYLEDSPIYLFDEWAADQDPSYRLFF
                     YRTLLPEMRKRGKIVIAITHDDHYFDVADRVYRMDGGRLDPYEQTTFETNMSYE"
     gene            4702..>4928
                     /locus_tag="VGQ51_06245"
     CDS             4702..>4928
                     /locus_tag="VGQ51_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036196.1"
                     /translation="MNKFGEFHLAENANIVSDTLPGERSAELLATQREMEGSIVSYPG
                     SMPIAIRRAKGAFVEDVDGNVFIDFFSFAGV"
BASE COUNT         1116 a         1231 c         1456 g         1125 t
ORIGIN      
        1 gcgatcgaag accggctgca aacggaaggc gccatcaaat ttcgcggggt gcatatcgat
       61 tccacggaaa cctttcagcg catcgtggat gccatctcga ataagttcct ccgatacatc
      121 gacggtaatt cgccaaggac caggctttcg gatcatgtgt acacttctac cgagtatgat
      181 cagacgcagc ggatcacgat gcacaacgag ctttcttatt ccgccaaatg gcccaataaa
      241 ctttttttta cctgtcttca gccggccgtc acgggtggcg aaacgctgct ggcggacagt
      301 cgtgagatcc tgcaacggat ggacccggac atcgtccggg aagtcgagca gcgggggatc
      361 acctatatca ggaatctcca cggaaacacg ggaatgggcc cttcctggca ggacacgttc
      421 gagacgaaag ataaacgcca ggcagaggac tattgcaaat cctacggcat gcagttcgag
      481 tggggggaaa gggatagtct ccggcttata cagccttcca ggggtatcct tcggcatagg
      541 gccacgggag aaaaggtttg gttcaatcag atcgaccaat tccatcccag ccatctggga
      601 gaagaaatgt acgaggtgat ccagctgatg tatggcgaac cggagaattt tccgatgtat
      661 gtacgctttg gggatgggac ggcgatcagc gcatctcttg tcggcgaggt gctgaaaacg
      721 atagacgagg tcacggcggc gccggtttgg gaaagggacg aattcctcct catcgacaat
      781 gagctcgtca gtcatgggag gaaccctttt agcgggcaga ggaaggtgct ggtagccatg
      841 tccgaatgat aaccgagcct tatggacctg gataaagaca atgtcatcgg tatcgtcggg
      901 ggaatggggc ctgtcgcggg tctcacactt tttcaccgga taacggagct gaccgccgcg
      961 gctgccgacc aggaacattt atccgtagtg atgatgtcct ttccccggca ttttgccgac
     1021 cgcaccgcat ttctcgacgg agagaccctt gtcaacccgg cctacaatgt cgccgagacc
     1081 atcggcaagc tggagcaggc aggggcaaaa gttgtgggca tcgcttgcaa tacctgtcat
     1141 acgcccgcga tattcgatat tatcctgcaa gagctgcata agcgcaggag ccggataaag
     1201 ctggtgcata tgctggcgga aacatgcggg tatatccgcg agaatcatcc cgacgtgcat
     1261 cgcgtaggcg tcatgtgtac caatggcacg tacaaatccg gaacatatgg ccggctgctg
     1321 cgggaatggg gctatgaggc cattctgccg tcggtcgatt ttcaggaagg cgtgatccac
     1381 aaaatggtat acgaccctgt cacgggcatc aaggccaacc ctaccatcct ttccccgtcc
     1441 acccattcgt acatggaacg ggcgcttcga tatttcgaag accggcaggc ggaggcggtg
     1501 gtgcttggat gtacggagct atctctttta cagttgacca gaggatacac cggcccgttg
     1561 gtcatcatcg actccaccga tgcgatggcc gaagggctga taagggcggc taagaacaat
     1621 cattcgacat gaagacccat ggcgtactat acagactgac cctgctttgg ctgtgggggg
     1681 cctgtcttcc gtattccctt ttttcacagg aacgcaaggc ggatagtctt atccgactgg
     1741 cggatcggga agttcccgcg tggatgcgtg ccggaaatat acccgggctg agcctggtat
     1801 tgatcataga cggtcacccg tatttccgca actatggttt tgcggacgtg gagaaaagga
     1861 ctccggtgac ctcacatacg cttttcgagc tgggttcctg cagcaaggcc ttcacggcgc
     1921 tggcggcggc acggctcatc ggggaaggga ggttgcatcc ggacgacccc gtatctaaat
     1981 atataccctg gtggcaagtg tcctataagg acacgccggt gacggtcacg ataggccaac
     2041 tgatgcatca caccagcggt atcccatgga gcaccatttc gaagataccc atcatctcgg
     2101 gaaaggatgc gctggagcag acggtaagac agctgcgggg acaggagctg aaccgccttc
     2161 ccggcaagaa attcgaatat gcaaccatca actacgatgt attggcgctg atcattcaac
     2221 gcgtggcggg tcagcccttc gagcaatatg ttcagcagca ggtgatcgat ccgctgcggc
     2281 tttcgcgtac gactatcggg tatcctatcg atcaggcacg aaaggccgag gggtataaga
     2341 tcggattttt caaggccagg aaatacgacg ctcctgtgtt cggtggcaat aatgccgccg
     2401 ggtatgtgaa tacggatgct gtcgatatgg cgaagtgggt gctattccag atagggctgg
     2461 aggatagcgc gctatatcct gcggcgcggt atacgcagca gcgggacgaa agcgtaccgc
     2521 ttcacggtat ggatagctat gcatccggct ggcaggtggc gctcgacggt acaggcgaga
     2581 tcttccatga agggaccaat ccgaacttct cttcgtatgt ggtattccgc cccgcgaaaa
     2641 agacaggtgt ggccatcgtc accaattcga atagcagcta cacgccggtg atcgggaaca
     2701 ggctgatcaa gctgctggcg ggggaaaaga tcgagaagga attcgacccc ggggacaact
     2761 tcgacaaggt gttctccgtg acatcccttc tcctgggagg atatgcgctg atcgtgctga
     2821 cttttttggt tctggttgtc ctcgacatta tccggcggcg aaggacctat cagggttatt
     2881 ccggaagcca actgcggaag atcgggttgc tgctgctggc gctgttgccc tatgcggccg
     2941 gactttacct ggccccgaag gccgtattcg gcttcacctg gcaggcgatg atcgtatgga
     3001 cgccggcaag cttcctcctt gcgctgtggc tgctgatctt ttgcatcgga ctgacctgcg
     3061 gggcatacgt gctttcggtg ggttttccgg agaagaacga gtacaggaga atgacgccca
     3121 agctattgtt gctgagcatc ctgtcggggc ttgccaacat gctgctcatc atattgatca
     3181 cctcttcgct ggatagcgaa gtgaagctgg gttacctggt gttctactat ttgctgatgc
     3241 tgtcggtcta cctgatgggc aggatgttcg tccaggtgag cctgatcaat ttcaccatgg
     3301 gcgtcattta tgagatcagg atgcggttga ccgacaagat attcgagacc tcctatcaga
     3361 aattcgagaa gatagacagg ggaatggtgt atacgaccat gaacgacgat gtcggcacca
     3421 tcggggactc gaccagtatg ttcgttgtgc tcgtgacgag catctttacg gccaccggcg
     3481 ccttattgta cctggcttcc atcgcattct gggctacttt gctgactttt ttcttcattg
     3541 cgagcctttc gacgctgtat tacctgatca tcaggagcat gaacaaatat tacgaacagg
     3601 cgcgggatac caggaatgta ttcatgcggt tgctcaacgg gatgatcgat gggtataaag
     3661 aactgtccct tcgccggaat aaaaagcagg gttaccggga ggacattgcg gccagcgccg
     3721 atgcttttag ggtgaagatg tccgtggcca gtatcaaatt tgtgaagaca tcgctcgtcg
     3781 gagaggcggt gctgatcgcg atactgggga tggtggcttt cgcttttccg aaggttttcc
     3841 cgggcattca ggcatatacg ctgatgagct ttattatcgt gttattatac ctgacagggc
     3901 ctgtcaatgc gatcgtgaat tcggtccccg gcattctgcg gttgagggta gccatccgcc
     3961 gtatccggca attcctccgg gatataccat cgggtgcgga taaaaagggc gggcgggtcc
     4021 tttccgcgtc tgccacggtg gacagcatcc gcgcagaagg gatcgttttc cggtatgacc
     4081 aggacgacag catacgtcca tttaccgtag ggccggtgaa cctggagatc ggcaggggag
     4141 agatcgtgtt catcatcggc ggcaacggca gtggaaaaac gacgttagcc aaacttttta
     4201 cgggactcta ccagccggag gagggacatt tcctgatcaa tggcgaggtc gtggaatcag
     4261 gctggctggg agaatatttt tctgcggtat tcagtcctgt tcatttattc gagaagctgt
     4321 atgccatcga tgtggaaggc cggtcggagg aagtgaacaa ctacctgaag ctgctggacc
     4381 tcgatcaaaa agtatccgtt cggcggaatg cctacacttc cctccggctt tcaggcgggc
     4441 aaaggaagag gttgggactg ctgcaatgtt acctggagga ttctccgatt tatctgttcg
     4501 acgaatgggc cgcggaccag gatccttcct atcgcctctt tttctacagg accctgctgc
     4561 ctgaaatgcg caagcgcggc aagatcgtta tcgccatcac ccatgacgac cattatttcg
     4621 acgtggccga tagggtatac cgtatggacg ggggcagact ggatccgtat gagcagacca
     4681 cattcgaaac aaatatgagt tatgaataaa ttcggggagt ttcatttggc ggaaaacgcc
     4741 aatatcgtat cggatacgct gccgggggaa cgttcggcag agcttttggc cacgcagcgg
     4801 gaaatggagg ggagcatcgt ctcctatccc gggagcatgc ccattgcgat ccgccgggcc
     4861 aaaggagcgt ttgtcgagga tgtcgacggg aatgtgttca tcgatttttt cagctttgcg
     4921 ggggtgtt
//
LOCUS       DASYSV010000204         8967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1317043, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000204 DASYSV010000000
VERSION     DASYSV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317043"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VGQ51_06250"
     CDS             complement(<1..705)
                     /locus_tag="VGQ51_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV9036197.1"
                     /translation="MIGKGFLTGVVFCLLMGGCKSHTKGVSQETPTSGTIHISVDESF
                     KPVIDSQIKVFESSFPNCHIIADYEPEAQCLRDLTTDSTRLVLVTRGLTVQEEDFYQD
                     SFHMRPRYGVLAFDAVAVVVNNSSRDSIFSIKQLQDLLSGKDKEHQPVMDGVTATSTV
                     RYAMDSILQGRPLGKNVMGVKGAEAVVDYVAGNPRAVGFVGASWIGDRSDPADTSFSR
                     KVRVGAVQCVSCKGPTN"
     gene            complement(710..952)
                     /locus_tag="VGQ51_06255"
     CDS             complement(710..952)
                     /locus_tag="VGQ51_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036198.1"
                     /translation="MEKEAPERPDRRKQSYALMRAIMDFGMGIIIVGFGILFLLAPLF
                     KLQLSIDDLFRYIFAGLCLLYGGFRIYRGARKNYFN"
     gene            1201..1704
                     /locus_tag="VGQ51_06260"
     CDS             1201..1704
                     /locus_tag="VGQ51_06260"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEV9036199.1"
                     /translation="MKVLFVCLGNICRSPLAEGILRQKALAAHLDWEVDSAGTNGYHT
                     GEAPHHLSQKVARLNGIDISRQRARQFTPADFQRFDKIYAMAGDVVDNIRRISGRSYD
                     PSKLSLLMNELHPGADEDVPDPWYGAEPGYHEVYKMISAAADRIIANYTQTTPEPPEA
                     ARPKVKH"
     gene            1712..3076
                     /gene="hisS"
                     /locus_tag="VGQ51_06265"
     CDS             1712..3076
                     /gene="hisS"
                     /locus_tag="VGQ51_06265"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV9036200.1"
                     /translation="MSKNNKPSLPQGTRDFDPVTVRKRRLIFDTIRDVFELYGFQPLE
                     TPAMEQLETLMGKYGEEGDKLIFKILNNGLGDPKNIDKSRKAFENVLAGKNDSDLTER
                     ALRYDLTIPFARYVAMNHGQLTFPFRRYQMQPVWRADRPQKGRYREFYQCDADVVGSN
                     SLLNEVELASIYQTAFQRLNIPVEIRINNRKILAALAELSGGADHLTDITVAIDKLDK
                     IGLEKVIIELTDRNLDASQISTVKNYLSIEGGNTEKLAALKTLFAGNDTGLQGITELE
                     TILDYPGAPPLVLDPTLARGLNYYTGTIFEVKAKDIAMGSIGGGGRYDDLTGTFGVPG
                     IPGVGISFGVDRIYDVMEELRLFPADIHTGTKVLFFHLGQAESRAAFGLLQQLRANGV
                     PAELFHEQAKFDRQFKYAEKKAIPFVVILGSQELAIRQCQLKNLSTGEQTTLGFDELI
                     RKFK"
     gene            3208..4827
                     /locus_tag="VGQ51_06270"
     CDS             3208..4827
                     /locus_tag="VGQ51_06270"
                     /inference="COORDINATES: protein motif:HMM:NF027446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HEV9036201.1"
                     /translation="MKKTILLLALLPAIAFSHAKAQTANPLFHHIPSDADQVYHVSLA
                     TLGPKVDWQTLSSLMKGRNLGKTKMPFDIMKALNSGVDFHQDVIIARSNVYHADSTRY
                     TTIIVHLTDSGKFVAFIQSAGSDMHFHQMPGHERVATGNKTSFAWNANLAVIVIGGAQ
                     LPGSTSTNPPDLHHLIHKALTALHGSDHSFFTTDTRFTTAFSNDADMQIWNRHATNLS
                     MMEKMLKGNPAAAKMGGIMQMIARSSVAPTIGTLRFEPGKISYNIVKFVSPDELSSLQ
                     RLEGQGLSGELVAAVPSGRLMGMMAMHENLHAMMDSVRRLIPMDSLNSMLGKKGLNLQ
                     DFLTAMKGDFMVLAYNPDKSAAGSAAAKPGIFGMVSVADPAAFTRITKGLNIPDAAAP
                     VDTSAADTAHHKLKIFYSLHNDIAVIGGDRRQVNDFFNHPAGDAKPAQRLMLDPSRPI
                     MFSMGIDMHSVADFLTPMLTRGDTIAAKNQALLDAIRQMDVLLISGGINAGTMQSDFQ
                     LRMTDKDRNGLSSFIDIMSKLSAKPSGNESQ"
     gene            4814..6463
                     /locus_tag="VGQ51_06275"
     CDS             4814..6463
                     /locus_tag="VGQ51_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036202.1"
                     /translation="MSRNSTGILITLLWFSCPRTFAQSPAVAPASSARSPAQSAAFRH
                     IPPDADAVYHVDLGAVLSKTSFSALEAPFLGSQAIAAEFGSIKSTLQEGIDLHSDLYL
                     AVSNRYHPDSPKYTTIVFTLMDPDKFIAHLRSQVRALRAPDGASPLPHGQGQVFTSSD
                     LAVAWDQRHAVLVTREASHSSLKSFGIDVIRDINYRRALAAWRGYPNSYFMTDPDFRK
                     AFSEKADAHIWNRHSSSFAALGEALRLTPLGAHLSGLYRGVEETREHNIGILRFDTGK
                     ISYRHISLTTPQKAASITRLVNPRFDSTLVTGAPFGNIIGAATFHFDPALLLDTLGKL
                     KELLFRQKGLTLQDMSAALKGDCLLLAYDASADQHSAETIQMPDVFAVFSVRDTAAFR
                     RSASYYRLTDAATAPDVDSSFNTPFHYYAVQNDIAVVTTTPAMARNYFSYSASASHPT
                     GRVLSKEMQNGSFSLGLDMGMFSDFFISLTKGKDATTIEKARNLAAVYRKMGVFTVSA
                     GHVIGNTVVTDFDLTLADPKKNGLAGLLEIFTGIMSIVNNR"
     gene            6510..7184
                     /locus_tag="VGQ51_06280"
     CDS             6510..7184
                     /locus_tag="VGQ51_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEV9036203.1"
                     /translation="MKKLVPKHPLSIRWMHWINFPVLFIMIWSGMLIYWANDVYKIHL
                     GRHILFGFFPESFYKALNISHRLAEGMAFHFLFMWIFFVNGLVYVGYTIFSGEWRYLF
                     PDRRSFKEAWLVLLHDLHIRKSAPPQKKYNGAQKIAYTGIILMGIGSVLTGLAIYKPI
                     QFSWLTAVFGGYGNARVIHFILTLLYCLFFLIHVVQVILAGWNNFQAMVTGFEVLHDT
                     KNQSNE"
     gene            7177..7986
                     /locus_tag="VGQ51_06285"
     CDS             7177..7986
                     /locus_tag="VGQ51_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV9036204.1"
                     /translation="MNKLRARLASFRTTLKKALGYSRDLTPEQQITRRSFLSFSVFAI
                     LGATAWRSWFLLKDSASKEGVQSPLRNSLDADGKIFKHTLSPDHLAPTYPTSAGATNV
                     RYNGGVGLNPSGFNAADWRLHVTTLDNRKLDISIEDLQRLPLTEFAFEFKCIEGWSQI
                     SWWGGVKFSDFIAHYGLQKEASLDYVGLSTPDDEYYVGIDMPSAMHPQTLLCYQMGGK
                     PLDPRHGAPLRLIIPIKYGVKNLKRIGKLYFSNDRPRDYWFERGYDYYCGL"
     gene            8128..>8967
                     /locus_tag="VGQ51_06290"
     CDS             8128..>8967
                     /locus_tag="VGQ51_06290"
                     /inference="COORDINATES: protein motif:HMM:NF044553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036205.1"
                     /translation="MIHIRTPKKILILLLILFGPFLVDAQINFQMLIIGGGGGGGRNN
                     GTNSSGGGGGGGGQVTLATSSAPQGAIINIVVGAGGYGYRFSPAVTQTNGNASSITFS
                     STTWTSAGGNAGSNATSSSSSGSGGASVNSDGSASGGRGGIYNNGNTASGWPTNGGNG
                     TSAYSTWGAATSTGQLSGSTYYYGGGGAGGNWGGQLGSGVAKAGLGGGGVSNSDAMAN
                     TGGGGGGANSNGHSGYDLPGGNGGSGLVIVAYPGSPQATGGTIVTTGGMTYHTFTSSG
                     TLTF"
BASE COUNT         2040 a         2943 c         2104 g         1880 t
ORIGIN      
        1 attcgtggga cccttgcagc tcacgcactg gacggcgccc actctgactt tccgcgagaa
       61 gctggtatcg gcaggatcgc tgcggtcgcc gatccagctg gctcccacaa accccacagc
      121 gcggggatta cccgccacat aatcgacgac ggcttctgcc cccttgacgc ccatcacatt
      181 ctttcccaac ggcctcccct gcaggatgga gtccatggca taacgtacgg tactcgttgc
      241 cgtgaccccg tccatcaccg gctgatgctc cttgtccttg ccgctcagca ggtcctgaag
      301 ttgtttgatc gaaaagatcg aatccctgga gctattattg acgacgaccg cgacggcatc
      361 gaaggccagc acgccataac ggggacgcat atggaaagag tcctggtaaa agtcttcctc
      421 ctgaacggta aggccacgcg tgaccagcac caggcgggtc gagtccgtgg tcaggtcgcg
      481 cagacattgc gcctcgggtt catagtcggc gataatatga caattgggga aggacgattc
      541 gaaaaccttg atctgggaat cgatgacagg cttgaaggat tcatcgacgc tgatatgtat
      601 ggtcccgctc gtaggtgtct cttgtgaaac tccctttgtg tgtgacttgc aaccgcccat
      661 aagcaggcaa aagacaactc ctgtcagaaa acctttaccg atcattaatt taattaaagt
      721 aattttttct cgccccgcga tagatccgga aaccgccgta cagcagacag agtccggcga
      781 atatgtaccg gaacaagtca tctatcgaca attgcagctt aaatagcggt gccagcagga
      841 aaaggattcc gaatccgacg ataatgatac ccatcccaaa atccataatg gcccgcatca
      901 acgcatagct ctgcttccgc ctgtccggtc tttccggagc ctctttttcc ataattaatt
      961 atatccttgc gggcaaatta agcaataaac gccaaaaacg cccggctaat ttacggtaaa
     1021 gagatgcagc accccgcctt ttccataaac ccgccgaccc ttcgatcctt cgctttgccc
     1081 ctccatcttc cccccaccat cgccccgcat tccttccgat cgcgctccat cttcccccca
     1141 tcgcccacgc attccctccg atcgccatcc atcgccccgc attccttacc tttgcccccc
     1201 atgaaagtcc tcttcgtttg tttgggcaac atctgccgca gcccccttgc cgaaggaatc
     1261 cttcgccaga aggcccttgc cgcccatctg gactgggaag tggacagcgc cggtaccaat
     1321 ggctaccaca ccggggaagc cccgcatcat ctctcccaaa aagtggcccg actcaatggc
     1381 atcgacatca gccgccaacg cgcccgccaa ttcacgccgg ctgattttca acgctttgat
     1441 aaaatatatg ccatggctgg ggacgtggtc gacaatatcc gccgcatctc cggtcgctcc
     1501 tacgatccgt ctaagctctc tcttctcatg aacgagctcc atccgggcgc cgacgaagac
     1561 gtacccgacc cctggtatgg cgccgaaccc ggctatcacg aggtctataa aatgatctcc
     1621 gccgccgccg accgcatcat cgccaactac acccaaacca cccctgagcc gccggaggcg
     1681 gctcgaccga aggtcaaaca ttagatcaac catgagtaaa aacaataaac cctccctgcc
     1741 ccagggcacc cgcgatttcg accccgtcac cgtgcgcaaa cgccggctta tcttcgatac
     1801 catccgcgac gtcttcgaac tatacggctt ccagcctctc gaaacccccg cgatggagca
     1861 gctcgaaacc ctcatgggca aatacggcga agaaggcgac aagctcattt tcaagatcct
     1921 caacaacggt ctcggcgatc caaagaatat cgacaaatcc cgaaaggcat tcgaaaacgt
     1981 cctcgccggc aagaacgact ccgatctcac cgaacgcgcc ctgcgctatg acctgacgat
     2041 acctttcgcc cgctatgtcg cgatgaacca tggccagctg acctttccct tcagaagata
     2101 ccaaatgcaa cccgtctggc gcgccgaccg cccccagaaa ggccgctacc gcgaattcta
     2161 tcaatgcgac gcagacgtcg tcggcagcaa ttctctgctc aatgaggtgg agctggcgtc
     2221 catctaccag actgccttcc aacggcttaa catccccgtc gagatccgca tcaacaatcg
     2281 caagatcctc gccgccctcg ccgaactatc cggcggcgcc gaccacctca cagacatcac
     2341 agtcgccatc gacaagctcg ataagatcgg tctggaaaaa gtcatcatcg aactcaccga
     2401 ccgcaacctc gacgcctccc agatctccac cgtaaaaaac tatctctcca tagaaggcgg
     2461 caacaccgaa aaactggccg ccctcaaaac ccttttcgcc ggcaacgaca ccggcctcca
     2521 gggcattacc gaactcgaga ccatcctcga ttaccccggc gcccccccgc tcgtcctcga
     2581 ccccactctg gcccgtgggc ttaactatta caccggcacc atcttcgagg taaaggcaaa
     2641 agacatcgca atgggcagca tcggcggcgg cggccgttat gacgacctca ccggcacctt
     2701 cggcgtaccc ggcatccccg gcgtcggaat ttccttcgga gtcgaccgta tctatgacgt
     2761 tatggaagag ctgcgcctct tccccgccga cattcacacc ggcaccaaag tccttttctt
     2821 ccatctcggt caggccgaat cccgcgccgc cttcggactg ctgcaacagc ttcgtgcaaa
     2881 cggcgtcccg gcagagctat tccacgaaca ggcgaaattt gaccgacaat tcaaatacgc
     2941 ggagaaaaaa gccattccct ttgtcgtcat cctgggcagc caggaacttg ccatccgcca
     3001 gtgtcagcta aaaaacctga gcaccggcga acagacgacc ctcggcttcg acgagttgat
     3061 ccgaaaattc aaatagtcgc cccgctcaaa tagtcgccac ccctcaaata gtcgctgcct
     3121 cgctcaaata ttcgtcacct cgctcaaata gtcgccgccc ctcaaaaacc cttgcataac
     3181 ccgcccgaat gccctaactt tcgtctcatg aaaaagacca ttctcctgct ggccctgctg
     3241 cctgccatag ccttttccca tgcgaaagcc caaaccgcca atcccctttt ccatcacatc
     3301 ccttccgatg ccgaccaggt ctatcatgtc agcctggcaa cgctcggtcc gaaagtcgat
     3361 tggcaaaccc tgtcctccct catgaaggga cgcaatctgg gcaagacgaa aatgcctttc
     3421 gacatcatga aggcattgaa ctccggggta gatttccacc aggatgtcat catcgcccga
     3481 tcgaatgtct atcacgccga tagtaccagg tatacgacca tcatcgtcca cctgaccgat
     3541 tccgggaaat tcgtggcctt catccaatca gccggcagcg acatgcattt ccatcaaatg
     3601 ccgggtcacg agagggttgc cacaggtaac aagacatcct tcgcctggaa cgctaacctg
     3661 gccgtcatag tcatcggagg cgcccaatta cccggctcca cctccactaa cccacccgat
     3721 ctacaccacc tgatccataa agcccttacc gccctccacg ggtccgatca ttcttttttt
     3781 actaccgata cccggttcac caccgcattc tccaacgatg ccgatatgca gatttggaat
     3841 cgtcatgcta ccaatctgtc catgatggaa aaaatgctaa aaggtaatcc cgccgccgcg
     3901 aaaatgggcg gcatcatgca gatgatcgcc agatcgagcg ttgctcctac catcggcacg
     3961 ctccgcttcg agcccggaaa gatttcctat aacatcgtca aattcgtctc ccccgatgaa
     4021 ctctccagcc tgcaacgtct cgaaggtcag ggtctgagcg gtgagctggt ggcggcggtc
     4081 ccatcgggca ggctcatggg tatgatggcc atgcatgaga atctacacgc catgatggac
     4141 tcggtccggc gcctgatacc aatggatagc ctgaacagca tgctcggcaa aaaaggtctc
     4201 aacctgcagg acttccttac cgccatgaaa ggcgacttca tggtgctggc ttataacccc
     4261 gacaagagcg ccgcgggtag cgccgccgct aaacccggca tcttcggcat ggtctccgtc
     4321 gctgacccgg ccgcttttac ccggatcacg aaggggctga atattcccga cgccgccgct
     4381 cccgtggaca cctcggccgc cgacacagct catcacaaac taaagatctt ttattccctc
     4441 cataacgaca tagccgtcat cggcggcgac cgccgccagg tcaacgactt tttcaatcac
     4501 cccgccggag acgccaaacc ggcccaacgg ctgatgctcg atccctcccg acccattatg
     4561 ttctcaatgg gcatcgatat gcattcggtc gccgatttcc tgacgcctat gctcaccagg
     4621 ggcgatacca tcgcggccaa gaatcaggcg ctgctcgacg cgatacgcca aatggacgtg
     4681 ctgctcatat ccggcggcat caacgcagga accatgcaat cggatttcca gcttcgcatg
     4741 accgacaaag ataggaacgg gctgtccagc ttcatcgata tcatgagcaa gttgtccgcc
     4801 aaaccatccg gtaatgagtc gcaatagcac cggtatcctg atcacgctgc tctggttttc
     4861 gtgcccccga acattcgcgc aatcacccgc cgtcgcaccc gcatcatccg cgcgctcacc
     4921 ggcgcaatcc gcggcctttc gccatatccc tcccgatgcc gacgcggtct atcatgtcga
     4981 tctcggagcc gtgctttcca agaccagctt ttccgcgctg gaggcgccat tcctcgggtc
     5041 ccaggccatc gccgccgaat tcggatccat caagtccacc ctgcaggaag ggatcgatct
     5101 ccattcggat ctatacctcg cggtatccaa ccgctatcac ccggatagcc cgaaatatac
     5161 cacgatcgtg ttcacactga tggatcccga taaattcatt gcccatttac gctcacaggt
     5221 aagggccctg cgcgcccccg atggcgcatc tccgttgcca catggccagg gccaggtatt
     5281 cacgtcctcc gatctggcgg ttgcatggga ccagcggcat gccgtgctgg tcacccgcga
     5341 agcctcgcat agttccttaa aatcgtttgg catcgatgtc atccgcgaca tcaattaccg
     5401 ccgggcactg gcggcctggc gcggatatcc gaattcttat tttatgaccg atccggattt
     5461 cagaaaggct ttttcggaga aggcggacgc acatatctgg aaccgccata gctcgagttt
     5521 tgccgcgctg ggcgaagccc tccgtctgac tcccctcggc gcccatctga gcgggcttta
     5581 ccggggtgtc gaggaaaccc gtgaacacaa tatcggcatc cttcgtttcg acaccggaaa
     5641 aataagttat cgccacatca gcctgacgac acctcagaaa gcagcttcca ttacgcggct
     5701 ggtcaaccct cgtttcgatt ccacgctcgt gacgggcgca ccattcggca atatcatcgg
     5761 tgcggcgaca ttccatttcg atcccgccct gctgctggat accctcggta agttgaaaga
     5821 acttttattc agacagaaag ggcttacgct gcaagacatg tccgccgcgc tcaaaggcga
     5881 ttgtctgttg ctggcctatg acgcaagcgc agatcagcac tctgccgaaa caatacagat
     5941 gccggacgtc tttgctgtct tcagcgtcag ggacacggca gcattcaggc gttcggcttc
     6001 gtactatcga ctcaccgacg ccgctacagc acccgacgtc gacagctctt tcaatacccc
     6061 ttttcattat tatgccgtgc aaaacgacat cgccgtggtg accaccacgc cggcaatggc
     6121 ccggaactat ttcagctact ccgcttcggc ctcccatcct accggccgtg tcctttccaa
     6181 agaaatgcag aacggctcct tctcgcttgg cctcgacatg gggatgtttt ccgacttctt
     6241 catcagcctc accaaaggaa aggatgcaac aaccatcgaa aaagccagga acctcgccgc
     6301 ggtctatcgt aaaatggggg tcttcaccgt ctccgcgggt catgtcatcg gcaacacggt
     6361 agtgacagac ttcgatctga cgctggccga ccccaaaaag aacggtctgg cgggcctcct
     6421 ggagatattc accgggataa tgagcatcgt aaataaccgg tagatcatct ttcccgcatt
     6481 aatcgtaaat ttcggtcaaa tcggaacgca tgaaaaagtt agttcccaag caccccctgt
     6541 ccattcgctg gatgcattgg atcaacttcc cggttctgtt catcatgatc tggagcggga
     6601 tgctcatcta ttgggccaat gacgtgtata agatccatct cggccgccat atccttttcg
     6661 gttttttccc ggaatcgttc tataaggcgc tcaatatatc ccaccggctg gcggaaggca
     6721 tggcctttca tttcctgttc atgtggatat tctttgtcaa cggtctggtc tatgtcggct
     6781 atacgatttt ttcaggtgaa tggcggtatc tctttcccga tcgccgctcg ttcaaagagg
     6841 cctggctcgt acttctccac gacctgcaca taagaaaatc cgcaccccct caaaaaaaat
     6901 acaacggcgc acaaaagatc gcctacaccg gtatcatcct gatgggcatc ggctcagtcc
     6961 tgacaggcct ggccatctac aaacccatcc agttctcctg gctcaccgcc gtcttcggcg
     7021 gctatggcaa cgcccgcgtc atccatttca ttttgacgct gctctattgt ctgtttttcc
     7081 tgatccatgt cgtacaggtg atcctcgccg gctggaacaa tttccaggcc atggtcaccg
     7141 gtttcgaagt cctccacgac acgaaaaacc aatccaatga ataagctccg ggcccgcctt
     7201 gcctccttcc gcacgacact taaaaaagcg ctcggctact cccgcgacct cacccctgaa
     7261 caacagatca cccgccgttc cttcctcagt ttttccgtat tcgcgatcct cggcgccact
     7321 gcctggagat cctggttcct gctgaaagac tccgcctcca aagaaggcgt ccaatctccg
     7381 ctccgcaact ctctcgacgc cgacggcaag atcttcaaac acaccctaag ccccgaccat
     7441 ctcgccccca cctatcccac ctccgccggc gcgaccaatg tccgctataa tggcggcgtc
     7501 ggcctaaacc ccagtggctt caatgccgcc gactggcgtc tccacgtcac cacgctcgac
     7561 aaccgcaaac tcgatatcag catcgaagac ctccaacgtc ttcccctcac cgaatttgcc
     7621 ttcgaattca aatgtatcga aggatggagc cagatctcct ggtggggcgg cgtaaaattc
     7681 agcgatttca tcgcccacta cggtctccaa aaagaggcgt ccctcgacta cgtcggactc
     7741 tccacccccg atgacgaata ctatgtcggg atcgatatgc ccagcgccat gcacccccag
     7801 accctgctct gctaccaaat gggtggcaaa cccctcgatc ccaggcacgg cgcgcccctc
     7861 cgcctcatca tccccatcaa atacggcgta aaaaatctca aacgcatcgg caaactctat
     7921 ttcagcaacg accgcccccg cgactactgg ttcgagcgcg gttatgatta ctattgcggg
     7981 ttgtaatccg gacggccctt gagcagattc tccaaatcgc gatcaccgtc cgcgcaggac
     8041 ttataccttc aatggatcat tccggatgtc ggacgtatgt gtccacagga gcggataatt
     8101 ttttttcgcg agaatcgttc tctttccatg atccatatcc gtacccctaa aaagatcctg
     8161 atccttctcc ttattctctt cggtcccttc ttagtcgatg cacaaataaa ttttcagatg
     8221 ctcatcatcg gcggcggtgg cggcggtggc cgcaacaatg gaaccaactc cagtggcgga
     8281 gggggcgggg gtggcggaca ggtgacactc gcaacatctt ccgctccgca gggagccatc
     8341 atcaacatag tagtgggggc cggcggttac ggataccgat tttcacctgc cgtgacccaa
     8401 acgaatggaa acgcttcctc tataacattc tcatcgacaa cctggacctc tgccggcggc
     8461 aatgccggat cgaacgcgac atccagctca tcatcgggca gcggaggcgc gtcggtcaat
     8521 tcggatggct cggcgtccgg tggacgcggc ggcatataca acaacgggaa cactgcctcc
     8581 ggctggccga ccaatggcgg caacggtacg tctgcatatt caacctgggg cgcggccacg
     8641 tcgaccggcc agttatccgg ctcgacgtat tactatggag gcggaggagc cggcggcaac
     8701 tggggtggcc agctcgggtc gggcgtcgcg aaggccggat tggggggtgg tggagtcagc
     8761 aactcggatg ccatggcaaa taccggcggc ggcggcggcg gagcgaattc aaacggacac
     8821 agcggatatg accttcccgg tgggaacggc ggatccggtc ttgtgatcgt ggcttatccc
     8881 ggcagcccgc aggccaccgg cggcacgatc gtgaccaccg gcggaatgac ctatcatacc
     8941 ttcaccagta gcggaacatt gacattt
//
LOCUS       DASYSV010000205        13377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2341964, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000205 DASYSV010000000
VERSION     DASYSV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13377
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341964"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VGQ51_06295"
     CDS             <1..371
                     /locus_tag="VGQ51_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEV9036206.1"
                     /translation="SRLAAWALASTYHRPGIACKNPSYARMETEGDKIVITCSDAGEG
                     LVVKGAAVKELFIAGEDRVFRPATVRIEGNKLVVSAAGVKNPVAVRYQFDNAGIGNVF
                     GKDGLPLAPFRTDDWFIVVD"
     gene            complement(442..1497)
                     /locus_tag="VGQ51_06300"
     CDS             complement(442..1497)
                     /locus_tag="VGQ51_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV9036207.1"
                     /translation="MVETILVVMLLLTLMLALYDVLRVFFGVLTFALIFSVSFAMPYE
                     WLSRRLGHRRKLAAVIYVIVLVAIVAIPLVFIISALRSHIKDLIAWMNTVRTNGLPPL
                     PHWISNLPLVGEDISDFWQQLQNSPREAIASHAEPIRVAMRNILTSGAGVVGTALQFV
                     IGIVISAFFLVAGESALAPIKSTMQHLLGRRDGLSLLKATTDAIRSVSIGVMGTAFIA
                     STISWLGLIIAGIHFAVLLSALVFFLVLIQLGPLLVWVPLVIWTASQGLTGTTIFLII
                     YGIVLLIIDGLLKPLLIAKSGRMPFLVLFLGVIGGLAAWGFTGMFKGAIILSVFYTLF
                     NSWLERRTTPVRPVSHR"
     gene            1624..2964
                     /locus_tag="VGQ51_06305"
     CDS             1624..2964
                     /locus_tag="VGQ51_06305"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEV9036208.1"
                     /translation="MHQVAVLALIQLLILLLPSPGAYRLFEKAGVPGWKAFVPLYNTW
                     IMLGLAGRLRHWIFWQLIPVAGWFVSMGIFIEFVKTFGKFLFYEHALAALVPVVYFVW
                     LGWDPAVAWKGPEAASTHRKSKTREWIDAAVFAVVAATLIRTFVFEPYVIPTGSMEKT
                     LLTNDYLFVSKFAYGPRLPMTPLSIPFVHNTLPVVGGRSYLEWIRVPYTRWFPRAIKR
                     GDDVVFNFPIGDTVIDLPDYQSQRPYYDVCRDLGHGNIDSGRQLVLQDPDQYPLVIRP
                     VDKEGNFIKRCVALPGDTLQIRDQIVYIDGSAQNLPPESESWYSVKTRGQPLDPGVLE
                     DEYGVDMSNGEEFRATAQTNVFSMRLTRRALEKMKAAGVAGQIEPELDSTWKVFPYSP
                     ECPWTQDNYGPVVIPRKGSAVYLTPANYALYERAIRVYEGNQLEKRPSGIYLNG"
     gene            2989..3150
                     /gene="lepB"
                     /locus_tag="VGQ51_06310"
     CDS             2989..3150
                     /gene="lepB"
                     /locus_tag="VGQ51_06310"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV9036209.1"
                     /translation="MDYYWMMGDNRHESMDSRFWGFVPEDHIVGRADLIWMSWNKGVR
                     WSRLFRRIH"
     gene            3472..6618
                     /locus_tag="VGQ51_06315"
     CDS             3472..6618
                     /locus_tag="VGQ51_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9036210.1"
                     /translation="MRRFLALLMWLLFYSVAALSQTRTVTGRVLSKDGQPIPDATVRI
                     DGTKTGTTTNAKGEFKLTARPGNVLIFSAISFSPERVTVTDGRDSYNIALTESAKFMD
                     EVVVSAGGLKTHVKEQGYAATAIKAEALTNTAPVDVTSSLAGKVAGLQISDVGGGVNP
                     NYRVVLRGQRSLLGNNQALIVLDNVVVPNSVLGNLNPDDIDNIEVLNGASAVALYGSE
                     ASNGALIITTKKGKKGAPAVKLSNTSTFQEISSYPKLQNEFGGGGNGYGVNPDGSPGF
                     SPIENQSYGPAFDGHMVNLGYPLENGDQLMVPYKANNDRSAFWQKSYTNQSDFSVSSG
                     SDNSTLYLSGQYATISGVMPGDHYNRATLRLNGTRKVSDKINTTYTLGWTQNRYNITT
                     GGGDIYNNLLNIPANVPITMFKDWKTNEYSNPNGFENPWYQNPYFEADNNRQKTRNDY
                     LIGNVQLDYAPVPSVNLTARVGMTTDNSSNLSSTDKFNYTPYAIKESGGSKSNIPGSV
                     TSSAGYYTRLEANVYGSWKKQYGNFNFNLTAGWSLRQNNSQATSASVSGLVVPGLFNL
                     SNSVNLPTANNQTYESRLLGAYGDFKIGYNNYLFVEVTGRNDWVSILNPPHNSFFYPS
                     VGLSFVASDAISALKDMRNLDYLKLRASWSQVGQVNLGADPNAVDFGAYRLQPTYNQI
                     NGYPYGGVAGYSVGDQVVQNGLLPEMTKGYEGGIDFSFLKSRISGSVTYYSTHTTNQT
                     VPVAISNASGYSSYLLNSGEVSNQGLETKLDLQVVQKRNWTATIGARYSYLNNKVISI
                     NPSLQTLPIAKYSSGTGAYAVPGLQFPVIMGTDYQRYQGHVIVDPISGLPEVNQALQV
                     LGSAAVKDNIGLDASVRYKQWNFYILFEYRGGNQIYNAGGPTYDWAGTSIRTVAFHRQ
                     RFIFPNSVIDQGNGKYITNTTAVIQNGNGNDGFWTDQTENMGVSSNYITSGAFWKLRQ
                     LSLSYSFPQKMLGNNKAIKNLTISVQGRNLFEWLPASNIYTDPEYSDAGSASNGIGLT
                     NLQAPPSRYYGGTLSITF"
     gene            6628..8211
                     /locus_tag="VGQ51_06320"
     CDS             6628..8211
                     /locus_tag="VGQ51_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEV9036211.1"
                     /translation="MNKLSILSISAIFLLGTSSCKKYLDINKNPNAATTATPEVILPQ
                     AIVYSAAVTSSFNNYGAELGGYAANAGGYGGFGSNWTYNFGPNDYSGLWSSTYDALND
                     LQAVQNFAVADKTDAHDYFGAIARIMKVYDFQLLVDTYNNIPYSNALLGASAVTPKYD
                     DAKTIYPALAAQLDSAIAIIAAANSKSGVTVPTSQQDPLFHGSMANWQKFAQTLKLRL
                     IIRASATVTFPNKTFDSNIGFLTTDAIVNPGYAKVSGQQNPSWNTWVQDYAGTTGNRA
                     WMPCTFVFSFYSGANLADPNRGTAIYYNFPATPNNQLGVGTTSVPSAPNVANAWYSGS
                     QSSLGNCPGIMKGLNMGEPLMLAAESYFLRAEADVRGILSDPAGSDKVNFRSGIAASF
                     HYLYLLPDDATIAPGWNVQTDTAQYYSDNAASYLVNYDLATTTDKQLEAIITQKYIAL
                     NFINSQEGWNEYRRTGYPKVSNFTSDPAKSFASTLSQSSRPDKLPTRILYPASEYSYN
                     AANVPTGISPYSSLIFWAH"
     gene            8244..9206
                     /locus_tag="VGQ51_06325"
     CDS             8244..9206
                     /locus_tag="VGQ51_06325"
                     /inference="COORDINATES: protein motif:HMM:NF020111.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 domain-containing protein"
                     /protein_id="HEV9036212.1"
                     /translation="MKQHKRAGLVLLVSLTLAQMSCLKDSPNNASPSGSNNVVEFQNS
                     SIPVSYTSVFPQYDNGISIKNDTGSFPVNLNYTGQISTAPQDIKITLATNQNALDSFN
                     SDQGTPYVMPPTDAISFSTSATIAKGTRQVTIRIVVNTAAPDFDYSSAYALPLTITQS
                     TYGIVSSNFGTAIYSFTPLNKYDGSYALTEKLTGWGAYSIDDGTTFKWPNNIGFVTSG
                     QFSNTTSDPNWGSLFLAFSPSGGTTAFGATTPQFTFDANDKLVSVVNTTPDDGRGRKL
                     AIDPAVTDSRYDASTKTIYAAFIMYQNGRPNQYVRDTLVYKSAR"
     gene            9264..9989
                     /locus_tag="VGQ51_06330"
     CDS             9264..9989
                     /locus_tag="VGQ51_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036213.1"
                     /translation="MRTLILTLPLTLLFFCARPQDHQTNKRNLSRHLRELYNVLKSDW
                     KVRDGGYTVVNDEGETIVKGAYADGRKTGVWNYYDDNGRLVQQYDFTNDSLISRDGDS
                     LSIVHSDYQIPGVIDDSATIRAPYKIGGPDYGFFLLYDERDIPAEIKSAASVAQMTYV
                     LTISAKGTLEAYRILFRGEGFGDISLSRSVKGLPHEASEFAAATVNGSPVRSQISWTI
                     PLDIKHFDHPGTNYFPTQKAPKD"
     gene            10095..10445
                     /locus_tag="VGQ51_06335"
     CDS             10095..10445
                     /locus_tag="VGQ51_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEV9036214.1"
                     /translation="MGSFSLIVLFIAGVAFAAGGIAVARFFVKGSTNPAKGQAYECGI
                     PAAGTPWNQFNVGYYLYALIFLIFDVELVFMYPWAIAVKKVGMASLVEIVIFLFILFM
                     GFLYAHKKGALKWM"
     gene            10436..10957
                     /locus_tag="VGQ51_06340"
     CDS             10436..10957
                     /locus_tag="VGQ51_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEV9036215.1"
                     /translation="MDVKQQNVPAQPFPGDVHPTAGGGILVTKLDEVINWARSNSLWP
                     LVFGTSCCAIEMMSTASAKYDWARFGFEVARATPRQADVIIIAGTIVNKMAPVLRRLY
                     DQMADPKYVIAMGACATSGGPFFYNTYSVVKGADHVIPVDVYVAGCPPRPEALLHALI
                     TLQEKIRNGHAAR"
     gene            10938..11399
                     /locus_tag="VGQ51_06345"
     CDS             10938..11399
                     /locus_tag="VGQ51_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEV9036216.1"
                     /translation="MDMQHDELKTRIGGMIPSAVFEDGSEWTTVLIDASVWEDLARHI
                     RSAAELEFDYLFCLTAVDWKTHLTVVYHLTSTVYRHTLVVKIKLDRQRPEVRTVSDIW
                     RTAEFHEREAFDLMGIVFMGHPDLRRLFMTDEWKGWPLRKDYEDPVNMIKL"
     gene            11416..12531
                     /locus_tag="VGQ51_06350"
     CDS             11416..12531
                     /locus_tag="VGQ51_06350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEV9036217.1"
                     /translation="MIEELGTTEQGELVVNLGPQHPSTHGVLHLVITLSGETIKKVEP
                     HLGYIHRSIEKMCESLSYRQFIYVTSRMDYLSSHINNHGCALCVEKGMQIEVPARAKV
                     IRVLMDELTRLASHQLWWGAMAMDVGALTPFFHAFREREAINDIMEETCGARLTMNYM
                     VPGGVMTDIHPEFQRKVKEFIQFFRGKIDEYDELVTGNVIFRSRMEGIGYLSREDALS
                     YGCTGPTARGSGVSCDIRKISPYEVYGEVQFEEIREQGGDCFARYLVRMQEMRQSLSI
                     IEQLIDNIPEGDFQAKTKAVLKLPKGEFFSKVETARGEFGVYIISEGGTTPYRIKFRS
                     PGFSNLSALDHMARGYKIGDLVAIMGTLDLVIPDIDR"
     gene            12558..>13377
                     /locus_tag="VGQ51_06355"
     CDS             12558..>13377
                     /locus_tag="VGQ51_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEV9036218.1"
                     /translation="MLSFEHITQSIDKWLYGVFPSGVAVVIEGLIVALCMIGLFAVLG
                     LVLVLMERKVSAYMQIRLGPNRVGPGGSLQTLADTLKLAMKEGLTPDGADKFLFNLAP
                     FLVIVVAMLILAPIAFARNVALWDLNIGVLYVSAISSLSVIGILMAGWASNNKYSLLG
                     AMRSGAQIVSYELSAGFSVLSIVVLTGSLRISDIIQSQRDGWWLFKGHIPAVIAFIIF
                     IIAVTAETNRAPFDLAEAESELTAGFHTEYSGMKFALFFLAEYVNIFIVCSIAAT"
BASE COUNT         3207 a         3591 c         3616 g         2963 t
ORIGIN      
        1 gcagccggct ggccgcatgg gcgctggcct ctacctatca ccggccgggc atcgcctgta
       61 agaacccttc ttatgcgcgc atggagacag aaggggacaa gatcgtgatc acctgctcgg
      121 atgcgggtga gggactggtg gtgaagggcg cggcggtgaa agagctgttc attgcgggcg
      181 aggacagagt gtttcggccg gcaacggttc gcatagaagg gaataagctc gtcgtttccg
      241 cggcgggtgt gaaaaacccc gtcgcggtgc gctatcaatt cgacaatgcg ggcatcggta
      301 atgtctttgg gaaagacggc cttccgcttg cgcctttccg gacggatgat tggtttatcg
      361 tcgtcgactg aagtgaagat cgcggatcga tgccggcggc tgatcgtcgc ccgaggatcg
      421 ctggtcgatg ccttcggctg atcaacgatg actgacgggc cggacggggg tagtcctgcg
      481 ctcgagccat gaattgaaca atgtatagaa caccgaaagg atgatcgcgc ctttgaacat
      541 cccggtaaaa ccccatgctg ccagaccgcc gataacccca aggaagagga ccaggaaagg
      601 catccgcccg ctcttggcga tcaggagggg cttcaggagg ccgtcgatga tcagcaatac
      661 gataccgtaa atgatgagga agatcgtcgt gcccgtcagg ccctggctgg ccgtccagat
      721 gaccagcggc acccatacca gcaaaggccc cagctggatc agcaccagga agaagacgag
      781 cgcggacagg agtaccgcga agtggattcc cgcaatgatg agaccaagcc aggaaatggt
      841 ggaggcgatg aacgctgtcc ccataacccc gatggatacg ctgcggatag cgtccgtcgt
      901 cgccttcagc agtgagagac cgtcccgtct tccaaggagg tgttgcatgg tggatttgat
      961 gggcgccagc gccgactcgc cggccactag aaagaatgcg gagataacga taccgatgac
     1021 gaattggagg gcggtgccca cgactcccgc gccactggtg agaatattgc gcatcgcgac
     1081 ccggatgggc tcggcgtggg atgcgatggc ttcccgggga ctgttctgca gttgctgcca
     1141 gaaatcgctg atatcttctc ctaccagcgg cagattggat atccaatggg gtaagggcgg
     1201 caggccattc gttcggacgg tattcatcca tgcgataagg tccttgatat ggctgcgcaa
     1261 tgccgatatg ataaagacca ggggtatagc gacgatagcc accaggacaa tgacatagat
     1321 gacggccgcc agtttcctgc ggtggccgag ccgtcttgac agccattcgt atggcatggc
     1381 aaaactgacg gaaaagatta gggcaaaggt caataccccg aagaaaacgc gcaatacgtc
     1441 atatagcgcc agcatgagag ttagcaacaa catcacgact aagatggtct ctaccatgct
     1501 tcgcggacta ttctgaaagg gtttttgtat cataggccgc catcggctaa aagttgctat
     1561 aaatttagaa ttatttaagt aatatttcgg gggaacctgc gaccaaaaca aaaagcaaaa
     1621 accatgcacc aggtcgcagt tttggccctg atccaattgc ttatcctgct cttgccctcg
     1681 ccgggcgcat atcgactgtt cgaaaaagcg ggagttccgg gatggaaggc cttcgtgcct
     1741 ttatacaata cctggatcat gctcggtctc gccggacgcc tccggcattg gatattctgg
     1801 cagctgatac cggtggcagg atggttcgtt tcgatgggga tattcatcga atttgtaaag
     1861 accttcggaa aattcctgtt ctatgagcat gcgctggccg cgctcgtgcc ggtggtatat
     1921 tttgtgtggc tgggctggga tcccgcggtg gcctggaaag gcccggaggc cgcaagcaca
     1981 caccggaaga gcaagacacg ggaatggatc gatgcggcag tattcgccgt ggtcgcggct
     2041 acgcttatcc gtacgttcgt tttcgagccc tatgtcatcc ctactggctc gatggaaaag
     2101 acgctgctga ccaatgacta tctttttgtc agcaaattcg catacggtcc ccgcctgccg
     2161 atgacgcctt tgtccattcc tttcgtgcac aacacactgc cggtcgtggg cggaagatcc
     2221 tatctcgaat ggataagggt accttatacc cgatggtttc cccgggccat taaaaggggt
     2281 gatgacgtgg tatttaattt ccccatcggc gatacggtga tcgacctgcc cgattatcaa
     2341 tcccagcgcc cttattacga cgtctgccgc gacctgggac atggaaatat cgactcgggc
     2401 cggcaactgg tcctgcagga tcccgatcaa tatcccctcg tgatccgtcc cgtcgacaaa
     2461 gaagggaatt tcatcaagcg gtgtgtagcg ctccccggcg acaccctaca gatcagggac
     2521 cagatcgtct atatcgacgg cagcgcccaa aatctgccgc ccgagtcgga gagctggtat
     2581 tcggtaaaga ccaggggaca gccgctggac ccgggggtcc tggaggacga atatggtgtg
     2641 gacatgagca atggcgaaga attcagagct accgcccaaa ccaatgtatt cagcatgcgg
     2701 ctgacccgcc gcgccctgga aaaaatgaag gctgccggtg tggccgggca aatcgaaccg
     2761 gagctggata gcacgtggaa ggtattccct tactcgccgg aatgtccctg gacgcaggat
     2821 aattatggcc ccgtggtcat cccccgcaag gggtctgccg tgtatttgac gcccgctaac
     2881 tacgccctct atgaaagggc catccgcgtc tatgagggaa atcagttgga aaagaggcct
     2941 tcgggtatct atctcaatgg ctaacctgtg gaccgttata cctttcgaat ggactattac
     3001 tggatgatgg gtgacaaccg tcatgaatcg atggatagcc ggttctgggg cttcgtgccg
     3061 gaagaccata tcgtgggaag ggccgatctg atctggatga gctggaataa gggggtccga
     3121 tggagccggt tgttccggcg gatccattag gttttagcgc tcgcaagcgg gtcccgtcac
     3181 ggtttgcaaa cttttgcaaa ttcacgcaaa acttatgcaa acggttgttt taagtccaaa
     3241 gatattataa ttaagtttaa ttaacatatc cttaacaatt ttagcagaaa gggataaaac
     3301 atgcactaac tgagggttag actgctaaaa cgccattgga tggtagtatt ctttgatttg
     3361 ttaattaacg aatatgttag ccgctgattt tggttcaacg aattacccta atttcatttt
     3421 aacgtttttt taaggtatct cccagaaaga cgataactaa aactactaca catgagaaga
     3481 tttctagcac tgttaatgtg gttgctattt tattccgtag cagccttgtc ccaaacaaga
     3541 accgttacag gtcgcgtact gagcaaagac gggcagccca tcccggatgc caccgtacgc
     3601 atcgatggga cgaaaacggg taccacaacc aacgcgaaag gcgaattcaa gctcactgca
     3661 cggccgggta acgtgctcat tttttccgcg atatcttttt ctcctgagcg tgtcaccgtc
     3721 accgacggca gggacagcta caacatcgca ctcaccgaga gcgctaaatt tatggacgag
     3781 gtggtcgtca gcgcgggcgg tctcaaaaca catgtaaaag aacagggcta tgcggccacc
     3841 gcgatcaagg cggaagccct caccaacacc gcacctgtgg atgtgaccag tagccttgcc
     3901 ggcaaagtgg cgggtctgca gatctccgac gttggcggtg gggtcaatcc caattatcgc
     3961 gtcgtcctcc gcggccagcg ttcgctgctg ggtaacaacc aggcgctgat cgtgctggac
     4021 aacgttgtcg ttcccaactc ggttttgggt aatctcaacc cggacgatat cgacaacatt
     4081 gaagtgctga acggcgccag cgccgtagcc ctttacggtt ccgaggcttc caacggcgcc
     4141 ttgatcatta cgacgaaaaa agggaaaaaa ggcgccccgg cggtcaagct ctcgaatact
     4201 tccaccttcc aggaaatatc ttcctatccg aaactgcaga acgaattcgg cggcggcggt
     4261 aacggctacg gcgtcaatcc cgacggcagc cccggcttct ccccgatcga gaaccagtcc
     4321 tatggtcccg ccttcgacgg gcacatggtc aatctgggtt atccgctgga aaacggcgat
     4381 cagctgatgg ttccctataa ggccaacaat gaccgcagcg ccttctggca aaaaagctat
     4441 accaatcaat cggatttcag cgtcagcagc ggcagtgata attcgacgct ctatctgtcg
     4501 ggacaatacg ccaccatctc cggcgtcatg cccggcgacc actacaacag ggctacgctg
     4561 cgtttgaacg gcacgcgcaa ggtatccgac aagatcaaca cgacctatac gctcggctgg
     4621 acccagaaca ggtacaacat cacgaccggc ggcggcgata tctataacaa tctgctgaat
     4681 atcccggcca acgtgcctat cacgatgttc aaggattgga agacgaatga atattccaac
     4741 cctaacggtt tcgagaaccc ctggtatcag aacccttatt tcgaagcgga caataaccgc
     4801 cagaaaacga ggaatgacta tctcatcgga aacgtgcagt tggattatgc gccggtcccc
     4861 tctgtgaatt tgacagcgcg ggtaggcatg accaccgata acagttcaaa cctgtcctcg
     4921 acggataagt tcaactatac accgtatgcg atcaaggaga gcggtggttc gaaatcgaat
     4981 atccccggtt ccgtcacctc gagcgccggt tattacaccc gcctggaggc taacgtttat
     5041 ggctcctgga agaagcagta cggaaatttc aatttcaatc tgacagcagg ctggtcactc
     5101 cgtcaaaata attctcaggc tacttcggcc tccgtatccg ggcttgtcgt ccccggattg
     5161 ttcaacctca gcaactccgt caacctgccg actgccaaca accagaccta tgaatcgcgg
     5221 ttgctgggcg catacggcga tttcaagatc ggctacaaca actacctctt cgtcgaggtt
     5281 accggaagga acgactgggt ttctatcctg aatcctccgc acaattcgtt cttctatcct
     5341 tccgtgggct tgtcgttcgt cgcctcggac gccatctcgg ctctcaagga tatgcggaac
     5401 ctggattacc tgaagcttag ggcaagctgg tcacaggttg gccaggtgaa cctgggagcc
     5461 gatcccaacg ccgtcgactt cggcgcttac cggctgcaac ccacctataa ccagatcaac
     5521 gggtatccgt atggcggcgt cgccggttac agcgtaggag accaggtggt tcaaaacggt
     5581 cttttgcctg aaatgaccaa aggatatgaa ggcggtatcg atttctcttt cctcaagagc
     5641 cgcatttccg gcagcgtcac ctattattcg acacatacga cgaatcagac cgttccggtc
     5701 gccatctcga atgccagcgg ctactccagc tatctgctga actccggtga ggtgagcaac
     5761 cagggtctgg agacaaaatt ggacctgcag gtcgtgcaga aaagaaactg gacggccacc
     5821 atcggcgcca ggtattctta tctgaataac aaggttattt cgataaaccc gtcgctgcaa
     5881 acgcttccca tcgcaaagta cagcagcggc accggcgcct acgcagtccc gggtcttcag
     5941 ttccccgtga tcatggggac ggactaccag cgctaccagg gccatgtcat cgtcgatccg
     6001 atctcgggtc tgccggaagt caaccaggcc ctgcaggtac tgggcagcgc agctgtgaag
     6061 gataatatcg gcctggacgc atcggtccgc tacaaacaat ggaacttcta catcctgttc
     6121 gagtaccgcg gaggcaacca gatctacaac gcgggaggtc cgacctacga ctgggcaggc
     6181 accagcatcc gcacggtggc cttccatcgc caacgtttca tcttccccaa ctcggtcatc
     6241 gaccagggca atggcaagta tatcacgaac acgaccgccg tcatccagaa tggcaatgga
     6301 aacgatggtt tctggaccga ccagacggag aacatgggtg tttcgtccaa ctatatcact
     6361 tccggcgcat tctggaaatt gcgtcagttg agcctttctt actccttccc acagaagatg
     6421 ctgggcaaca acaaggcgat caagaatctc acgatctccg tacagggccg gaatcttttc
     6481 gaatggctgc cggcatcgaa catctacacg gatccggaat acagcgacgc gggctcagcc
     6541 agcaatggta tcggtcttac gaacctgcaa gctccgccat ccaggtatta tggcggtacc
     6601 ctttctatca ctttttaatc gtgtaacatg aacaaacttt caattctatc catatcggcg
     6661 atcttccttc tcggcacctc atcgtgcaag aaatatctcg acatcaataa gaaccccaat
     6721 gccgcgacca cggcgacgcc ggaagtcatc cttccccagg ccattgtgta ctctgcggcg
     6781 gttacttcca gcttcaataa ctatggcgcg gagctcggag gatatgcggc gaatgccggc
     6841 ggctatggcg ggttcgggtc caactggacc tataacttcg gaccgaatga ttactccggt
     6901 ctttggtcat ccacctatga cgcgttgaat gacctgcagg ccgtacagaa ctttgcggta
     6961 gcagacaaaa cggacgccca tgattatttc ggcgcgatcg cccggatcat gaaggtgtac
     7021 gatttccagc tgctggtaga cacgtacaac aacataccct actcgaacgc gttgctcgga
     7081 gcgtcggcag tgacgcccaa atatgacgac gccaagacga tttatcccgc cctggcggct
     7141 caactggatt cagcgatcgc gatcatcgcc gccgcgaata gcaagtcagg tgtcacggtg
     7201 cccacttccc aacaggaccc attgttccac ggatcaatgg cgaactggca gaagtttgcg
     7261 cagacgctca agctacggtt gatcatacgg gcttcggcga cggtcacttt cccgaacaag
     7321 acattcgaca gcaatatcgg gttcctgact acggatgcaa tcgtcaatcc cgggtatgcg
     7381 aaggtcagcg gacagcaaaa tccttcatgg aacacgtggg tacaggacta cgctgggacg
     7441 accggaaaca gggcatggat gccatgtacg tttgtcttct ctttttacag cggggccaac
     7501 cttgccgatc ccaaccgcgg aacggctatc tactataatt tccccgcgac ccccaataat
     7561 cagcttggcg tcggcacgac ctcggtgcct tccgcgccaa acgtggccaa tgcctggtac
     7621 tcgggcagcc aaagctcgct tggcaactgc cccggtatca tgaaaggcct caacatgggc
     7681 gaaccgctga tgctggccgc ggaaagttat ttcctgcggg ccgaggccga tgtacgtggg
     7741 attctatccg atccggcggg ttccgacaag gttaatttca gaagcgggat cgctgcgtca
     7801 tttcattacc tctacttgct gccggacgat gccacgatcg ccccggggtg gaacgtgcag
     7861 accgataccg cccaatacta ttccgacaat gcagccagtt acctggtcaa ttatgatctg
     7921 gccacgacga cggacaagca attggaggcg atcatcacgc agaaatatat cgcactgaac
     7981 ttcatcaata gccaggaagg ctggaatgag taccgccgga ccggttatcc gaaggtgtcg
     8041 aattttactt ccgatcccgc aaaatccttc gcgtctactt tgtcgcaaag ctcgcgaccg
     8101 gataagctgc cgacccggat cctgtacccc gcctcggaat attcgtataa cgcggcgaat
     8161 gtgcccacag ggatcagccc gtattcgtca ctcatattct gggcccacta atgaaatcaa
     8221 atctttattc aaaatcattc aatatgaagc aacataaaag agcaggtctt gtattgctgg
     8281 tatcccttac cctggcgcag atgtcctgtt tgaaggattc tcccaacaat gcatctcctt
     8341 ccggttccaa caacgtggta gaattccaga acagttctat tcccgtgtcc tatacgagcg
     8401 tttttccgca atacgacaat gggatcagca tcaaaaacga cacggggagc ttccccgtca
     8461 acctgaacta tacgggtcag atcagcacgg ctccgcagga tatcaagatc acgctggcta
     8521 cgaaccagaa tgcgttggat tcgttcaaca gcgaccaggg gacgccttac gtgatgccgc
     8581 ccacggatgc tattagcttt tctacttcgg caacgatcgc caaaggcacc cgccaggtta
     8641 cgatccggat tgtcgtcaat accgccgctc ccgacttcga ctactcttcc gcctatgcgt
     8701 tgccattgac catcacgcag tctacatacg ggatcgtcag ctcgaatttc gggaccgcta
     8761 tctattcctt cacgccgctg aataaatatg acgggtctta tgcgcttacc gaaaagctga
     8821 ccgggtgggg cgcatacagc atcgatgacg gtacgacttt taagtggccg aacaatatcg
     8881 gtttcgtcac atccggccag ttttcgaata cgacgtccga tccgaactgg ggatcgcttt
     8941 tcttagcctt ctccccgtcg ggagggacca ctgctttcgg cgcaactacg ccgcagttta
     9001 cattcgatgc taatgacaag ctggtgtccg tcgtcaatac tacacccgac gacggccggg
     9061 gaagaaaatt agcgatcgat cccgctgtga cagatagccg ctatgacgcc agcaccaaga
     9121 cgatctatgc tgcgtttatc atgtatcaga atgggcggcc gaaccaatat gtacgcgata
     9181 cgctggttta taagagtgca cgatagaatt tgactcaggg gccgccgaaa ggcggccctt
     9241 tttctattaa tttgaaccat taaatgagaa cgctaattct gactttaccg ctcaccctgc
     9301 tgttcttttg cgctcgccct caggatcacc agaccaacaa aagaaatctt tcgcgccatc
     9361 tgcgcgaact ctataatgtc ctgaaatccg actggaaggt cagggatggc ggttataccg
     9421 tggtcaatga cgagggcgaa accatcgtaa agggcgccta tgcggacggc cggaagaccg
     9481 gcgtatggaa ctactacgat gacaatggcc gcctggtcca gcaatacgat ttcaccaacg
     9541 acagcctgat ctcccgggac ggagattctc tctccatcgt gcatagtgat tatcaaatac
     9601 cgggcgtcat cgatgattcc gcaacgatcc gggcgcctta taaaatagga ggaccggact
     9661 atgggttctt cctattgtat gacgagcggg atattcccgc cgaaattaag agcgccgctt
     9721 ccgtcgcaca gatgacgtat gtcctgacga tctccgcaaa gggaaccctc gaagcctacc
     9781 ggatcctttt caggggcgaa ggtttcggcg atatctccct ttccagatct gtaaagggtc
     9841 tgccgcatga agcctccgag ttcgcggcag ccacggtgaa cggcagccct gtccgaagcc
     9901 agatcagctg gaccattccg cttgatataa agcatttcga tcaccccggc acgaactatt
     9961 ttccgacaca aaaggctccg aaggattagc ggtgactgcg ggggtgtcga aggaaggagg
    10021 acagcggacg gcatggatta aatatcgatt taccaaaaaa tcggtatatt agtaggggtc
    10081 aaaaccctct acccatggga agcttttcac tcatcgttct tttcattgcc ggggtcgcct
    10141 ttgcagcggg tgggattgcc gttgcacggt tcttcgtaaa aggcagcacc aatccggcaa
    10201 aaggtcaggc ctacgaatgc ggtatcccgg ccgccggcac tccctggaat cagttcaatg
    10261 tcggatatta tttgtatgcg ctgatctttc tcatcttcga tgtggaactt gtcttcatgt
    10321 atccctgggc catagccgta aagaaggtgg ggatggcatc cctcgtggag atcgtcatct
    10381 tcctgttcat cctgttcatg ggatttttgt atgcgcataa aaagggagcg ttgaaatgga
    10441 tgtaaaacag caaaacgtac cggcgcagcc gtttcccggg gacgtccatc ccacggcggg
    10501 cggagggata ctggtgacta agttggacga ggtgatcaat tgggcaagat cgaattccct
    10561 gtggccgctg gtgttcggca cttcctgctg cgccatcgaa atgatgtcca ccgcctccgc
    10621 caaatacgac tgggccaggt ttggattcga agtggccagg gctactccac gacaagccga
    10681 cgtcatcatc atcgccggaa ccatcgtcaa taagatggcc cccgtactgc gacgattgta
    10741 cgaccagatg gccgacccca aatacgttat cgccatgggt gcctgcgcca ccagcggagg
    10801 accgtttttc tacaatactt attcggtggt gaaaggcgcg gatcatgtga tccctgtaga
    10861 cgtctatgtg gcgggatgcc cgccgcggcc cgaggccttg cttcacgcgc tgatcaccct
    10921 acaggaaaaa atcaggaatg gacatgcagc acgatgaatt gaaaacaagg ataggcggga
    10981 tgatcccatc ggccgtcttt gaagacggga gcgaatggac gacggtgctc atcgatgcga
    11041 gtgtctggga agatcttgca cgacatatcc ggtcggcggc agagcttgaa ttcgactacc
    11101 tgttttgtct gactgccgtc gactggaaaa cgcatctcac agtagtttat catctgacat
    11161 ccaccgtcta tcggcataca ctggtagtga agatcaaact ggatcggcag cgcccggaag
    11221 tgcgcacggt atccgatatc tggcgtacgg cggaattcca tgagcgcgaa gcattcgatc
    11281 tcatgggcat tgtttttatg ggccatcccg atctgcgccg gctgttcatg acggatgaat
    11341 ggaaagggtg gccgctaagg aaggattacg aggaccctgt gaatatgata aaattatgat
    11401 cgaaggagtc taactatgat cgaagaactc ggcacaacgg aacaaggcga gctggtggtc
    11461 aacctgggcc cacaacatcc ctccacccat ggggtgctgc acctggtcat tacgctcagc
    11521 ggggagacga tcaaaaaggt cgagcctcac ctcggttata tccatcgcag catcgaaaag
    11581 atgtgcgaaa gcctgtccta ccggcaattc atctacgtta ccagccggat ggattatctt
    11641 tcttcacata tcaacaatca cggatgtgcg ctttgcgtcg aaaaggggat gcagatcgag
    11701 gtgccggcaa gggcaaaggt gatcagggta ttgatggacg agcttacacg gctggcctct
    11761 catcagctct ggtggggagc aatggcgatg gatgtcggag cgctgactcc ttttttccat
    11821 gcgttcaggg aaagagaggc gatcaacgat atcatggagg agacctgcgg agcgaggttg
    11881 acgatgaatt atatggtacc cggcggggta atgacggata tccatcccga atttcaacga
    11941 aaagtgaagg aattcattca gttcttccgg ggaaagatcg acgagtacga tgaattggta
    12001 acgggaaacg ttatcttcag gagcaggatg gagggcatcg gatatctttc ccgtgaggat
    12061 gccttgtcat atggttgtac ggggccgaca gcccggggta gcggtgtgag ctgcgatata
    12121 agaaaaatat cgccatatga agtctatggc gaggtgcaat tcgaggaaat tcgcgagcag
    12181 ggtggagatt gttttgcgcg ttacctggta cgtatgcagg aaatgcggca atcgctgtct
    12241 atcatcgaac aactgatcga taatatcccg gaaggcgact ttcaggcaaa gaccaaggca
    12301 gtgctgaagc tgccaaaagg cgagttcttt tcgaaggtag agacggctcg cggcgagttt
    12361 ggagtgtata tcattagcga aggcggaacg acgccctata ggatcaagtt ccgttcgccc
    12421 gggttttcca atctatcggc gctggatcac atggcgcggg gatataagat cggcgattta
    12481 gtggcgatca tggggacgct ggatctcgtg attccggata tcgatcgtta aaggccaccg
    12541 ggccgaattg aaaaacgatg ctgagctttg aacatataac gcaatccatc gacaaatggc
    12601 tgtacggggt attcccgtcc ggcgtggccg tcgtgatcga aggcctgatc gtcgcgcttt
    12661 gcatgatcgg tcttttcgcg gtgctgggac tggttctcgt cctgatggaa agaaaggtct
    12721 ccgcatatat gcagatacga ctgggtccga acagggtagg gcccggcgga agcctgcaaa
    12781 cgctggcaga cacgctcaag cttgcaatga aagagggact gacccccgac ggcgccgata
    12841 aattcctttt caatctcgct cccttcctgg tgatcgtcgt ggcaatgctg atccttgccc
    12901 cgatagcctt tgcgcgcaat gtggcgttat gggacctcaa catcggggtg ttgtatgtct
    12961 cggcgattag cagcctttcc gtcatcggta tcctgatggc cggctgggcc agcaataata
    13021 aatattcact gttgggcgcc atgcgcagcg gcgctcagat cgtcagctat gaattgtcgg
    13081 cgggtttctc cgtcctttcg atcgtcgtgc tcaccggcag tctgcggata tcggacatca
    13141 ttcaatccca acgcgacggc tggtggctgt tcaagggaca catccccgcg gtcatagcct
    13201 tcatcatctt catcatcgca gtgacggcag aaaccaaccg cgcacccttc gatcttgccg
    13261 aggcggagtc ggagctgacg gcagggttcc atacggagta ttcaggtatg aagttcgcgc
    13321 tgttctttct ggccgaatac gtcaatatat ttatcgtatg ctcgatcgcc gccacat
//
LOCUS       DASYSV010000206         7695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_934190, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000206 DASYSV010000000
VERSION     DASYSV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934190"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VGQ51_06360"
     CDS             <1..1059
                     /locus_tag="VGQ51_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV9036219.1"
                     /translation="DIPAEERDYYLERRYPAYGNLVPRDVASRAAKERCDAGYGVGTS
                     RQAVYLDYASAIERYGKTECRKHGIENPTPEEIRKHGVEVVKEKYGNLFDMYNKITGE
                     DPYSTPMRIYPAVHYTMGGLWVDYELMTTVPGLYALGEANFSDHGANRLGASALMQGL
                     ADGYFVIPYTVGNYLAGEIGTKPIETTHESFDAAEKAVADRIARLMSIKGSQSPESFH
                     KRLGKIMWDKCGMARNEAGLKTAIEEIRALKVEFWRDLRITGGVDEFNPELDKAGRVA
                     DFIELGELMCQDALDRRESCGGHFREEMQTEEGEAKRDDDHFLFVSAWEYKGENNWEL
                     HKENLEFEVAHPVQRSYK"
     gene            1070..1834
                     /locus_tag="VGQ51_06365"
     CDS             1070..1834
                     /locus_tag="VGQ51_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEV9036220.1"
                     /translation="MEHYTMNLTLKVWRQKNKQDKGGFVTYTVSGISSEMSFLEMIDV
                     LNERLVAEGNDPIAFDHDCREGICGMCSMYINGRPHGPWEGTTTCQLHMRAFKDGDTI
                     VVEPWRAKAFPVIKDLVVDRSAFDRIIQAGGYISVNTGNAVDANSIPIEKDKADTAFA
                     AAACIGCGACVAACKNSSALLFVSAKVSHLALLPQGDPERKTRVWDMIAQMDKEGFGS
                     CTATGACEAVCPKEISIQNIARLNKEYGRSGATTDK"
     gene            1923..3575
                     /locus_tag="VGQ51_06370"
     CDS             1923..3575
                     /locus_tag="VGQ51_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9036221.1"
                     /translation="MVRVNNVTLSFGKRVLFDEVNLTFTKGNCYGVIGANGAGKSTFL
                     KILASEIDPTKGNVEITPGERMAVLNQNHFAFDDSTVLNTVLMGHQRMWTVMQERDAI
                     YAKADFSEEDGIRAGELEGEFGEMGGYTAESDAASLLSDLGVKEALHNVLMKELPGAL
                     KVRVLLAQALFGNPDILILDEPTNDLDVETINWLENFLADYANIVIVVSHDRHFLDAV
                     CTHVADVDRQKIKIFTGNYTFWYESSQLMARQLSDKNKKTEDKRAALLEFIARFSANA
                     SKSKQATSRKKALEKLTIEEIEPSNRRYPGIIFKQLREVGNQILNIEKLSKKAEGGHT
                     LFSNVSFSVNKGDKIAFVSKDHLALTSFFDIINGQLHADSGKLEWGTTVTKAYLPNDN
                     TEYFEGQHINLMDWLRQFAPPHVTDVDEPFLRGFLGKMLFSGDEVLKNTNVLSGGEKV
                     RCMLSRMMLQDPNVVILDEPTNHLDLESIQAFNESMSNFKGIVLLTSHDHTFLQTVAN
                     RVIELTPSGIIDRLMSFDEFLEDARVKQLREEMYPREELVKA"
     gene            complement(3565..4500)
                     /locus_tag="VGQ51_06375"
     CDS             complement(3565..4500)
                     /locus_tag="VGQ51_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036222.1"
                     /translation="MPKRTYFLPIRCLAAAVAFSGCIPVREQAFYVSPLNGNSEDYHT
                     LPLIKDSAPMAIYARTAFYWGSANTRHNDYLDGWSFSGYAAHHFGRFQCYYGLDLSLG
                     TYIVGKWDTSRTFGGLLSTRYWLPPSGSRQLAAFAGHKFYGSTSFSGGINAVMPLGRG
                     GEWRFLGLETSLHREFGDYLSFRQQLPDSLVTQVVKSRLFGTLGVTTEIIGRTSYGEW
                     GFRIATGGVLGSPYNHLNIFDSVKNVQLRAYTYFDLSLHFTFGRITSYFQMDAATHAM
                     TGRLGVVFRMTSPRVPAKRSRRYHFEKKDKGLIMP"
     gene            4624..>7695
                     /locus_tag="VGQ51_06380"
     CDS             4624..>7695
                     /locus_tag="VGQ51_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036223.1"
                     /translation="MIRRLLISILLFSGASLSTRAQLATSDSLRRMTDSLRRTDSLQR
                     ATDSLHRADSLHRADSLRKVRSDTLLSATSMLHQIDSLNAAPAAPIDSVKLRFDAMMN
                     GGAARPGDAPGTAVSSGAGPPRSAPPGPPRSGPPTTRKSGPAKPVILSNLHRKYIPVR
                     PGRVPIDSMSLIPGSFLMKGVGDSAYTLDWVNGVVLWIHPPAATDSVLVTYRSFPYRM
                     NAVARRFSYDSIESYMLVRPYDKGKDDVADNFFNFGNMTYNGSFGRSITFGNSQDAVV
                     TSNLNLQISGYLADSIQISAAITDNNIPIQPDGTTANLNEFDQVFLQFKKKNWALTMG
                     DIDLRQNQLYFLNFYKRLQGASFEATEQLGPRSTNKTLVSAAIAKGKFARNIFQGQEG
                     NQGPYLLHGDNNELYFIVLAGTEKVFIDGQLMQRGEDQDYTINYNTAEITFTPNRLIS
                     QDARIQVEFEYADRNYLNVNLYAYDEAVIAGKLKLRLGLFSNSDARNSPINQSLTPDE
                     NKFLARLGNDIQHAYYPVANVDTFAAGKVLYKKVDTTYKNAAGVVVHDSVFVFSTDAS
                     QTLYNLSFANVGPGYGNYIPDLSGINGAVYDWVPPVNGVPQGTFEAAEYLVTPKTQQI
                     VSLGADYNINKHTTVTTELAHSHYDINTLSTLGKYNDDGNAAKVTFRNVKTLGSAAKG
                     LQLTSTAGFEYVQANFQPVQRLRDPEFDRNWGMSLSVNPADEKIFNAGVQLSDKKLNA
                     VKYEISRYERSDGFTGMQNNISSRQTVLGWKIIDQLSLTTYDSSHRFTGYYLRPSVEV
                     SRKLSFLKDYVISGGYSLEDNNNKDRSTDTVSATSYAFHTWTASLKSPEKLPNKWGMV
                     YSERTNSYPGGPSLLVGDRSKTFNVFADLLKNPHHQFHVNATYRQMTIVDTSLALHTV
                     DSATAFRGGDKTLVGRVEYVVNGWKGLLRGNLLYEVGSGQQQKLAFTYLQVPAGTGQY
                     AWIDYNADGIQQLGEFVLAQFQDQAQYIRVYTPTAEYIKANYNTFNYSFSLN"
BASE COUNT         1886 a         2063 c         2162 g         1584 t
ORIGIN      
        1 gatatcccag ccgaagaacg ggattattat cttgaaagac ggtatccagc ctatgggaac
       61 ctggtgccgc gcgacgtagc gtcacgtgcg gctaaggaac gctgtgatgc gggctatggg
      121 gtaggcacgt ccagacaggc cgtctatctc gactatgcat cggcgatcga acggtatggg
      181 aagaccgaat gccgcaaaca cggcatcgaa aacccgacgc cggaggagat ccgtaaacac
      241 ggcgtagagg tggtcaaaga gaaatatggc aacctcttcg atatgtacaa caagatcacc
      301 ggcgaggacc cttattcgac gccgatgcgg atctatcccg ccgtgcatta tacgatgggt
      361 ggcctatggg tcgattacga gctgatgacg accgtgccgg gtctgtatgc ccttggtgaa
      421 gccaatttca gcgatcacgg cgccaaccgg ctgggagcct ccgcgctcat gcagggtctg
      481 gccgacggct atttcgtcat tccttatacc gtcgggaatt acctcgcggg cgagataggg
      541 accaagccga tcgagactac gcatgaatcg ttcgatgccg cagaaaaggc ggtagccgac
      601 aggatcgccc ggctcatgag catcaaggga tcgcaaagcc ccgaaagctt tcacaaacga
      661 cttgggaaga tcatgtggga taaatgcggc atggcccgga acgaagccgg tctgaagacg
      721 gccatcgaag agatacgcgc cctgaaagtg gaattctggc gcgatctgcg gatcacaggc
      781 ggtgtcgatg aattcaaccc cgagctggac aaggcggggc gggtggccga ttttatcgaa
      841 ctcggcgagc tgatgtgcca ggatgcgctg gatcgccggg agagctgcgg cggacatttc
      901 cgcgaagaga tgcagaccga ggaaggagag gccaaacggg acgacgacca ctttttgttc
      961 gtatcggcct gggaatataa aggtgagaac aattgggaat tacataaaga aaacctggag
     1021 ttcgaagtcg cgcaccccgt gcagcgcagc tataaataaa taacagcata tggaacacta
     1081 tacgatgaac ctcacgctca aggtctggag acagaaaaac aagcaagaca aaggcgggtt
     1141 tgtgacgtac accgtcagcg gaatttcctc cgaaatgtcc tttttggaaa tgatcgacgt
     1201 gctgaacgaa cgactggtgg cggaaggtaa cgacccgatc gcattcgatc acgactgcag
     1261 ggaaggcatc tgcggcatgt gttccatgta tatcaacggg cgtccgcacg gaccgtggga
     1321 gggaacgacc acctgtcagc tgcatatgcg cgccttcaaa gacggcgata ccatcgtcgt
     1381 cgagccctgg agggcaaaag ccttcccggt gataaaggac ctggtagtag accgcagtgc
     1441 gttcgaccgg atcatccagg cgggcggcta tatctcggta aataccggga atgcagtaga
     1501 tgccaattcc attcccatcg aaaaagataa ggcggacact gcctttgccg ctgcggcctg
     1561 tatcggctgc ggcgcctgtg tcgcggcttg caagaacagc tctgcgctgt tattcgtctc
     1621 cgccaaggtc tcgcacctgg ccctgttgcc ccagggcgac ccggaacgca agaccagagt
     1681 ttgggatatg atcgcccaga tggataagga agggtttggg agttgtacgg ctaccggcgc
     1741 ctgtgaagcg gtctgcccca aagagatatc gatccagaat atcgcccggc tgaacaaaga
     1801 atacggcagg tcaggggcta cgaccgataa atagggggcc gcgcatcctt tgccggcagg
     1861 cgcaaatgtg ttacctttgc gcccaccaaa gccgctggag gcggcttcaa ttaaattagc
     1921 gcatggtcag agtgaacaat gtcacccttt ccttcgggaa aagggttcta ttcgatgaag
     1981 tcaatctcac gtttacaaaa gggaactgtt acggggtcat cggcgccaat ggggccggga
     2041 aatcaacttt tttaaagata cttgcgagcg agatcgatcc gacgaagggg aatgtggaga
     2101 tcacccccgg cgagcgtatg gcggtcctga accagaacca cttcgctttt gatgactcga
     2161 ccgtactgaa tacggtattg atgggtcacc agcggatgtg gacggtaatg caggaacgcg
     2221 atgccatcta tgcaaaggcc gatttctcgg aagaggacgg tatccgtgcc ggtgagctgg
     2281 aaggcgagtt cggagagatg ggaggctata cggcggaaag cgatgcggcg tcgcttctga
     2341 gcgacctggg agtcaaagaa gccctgcaca atgtgctgat gaaggaactt cccggcgcgc
     2401 tgaaggtcag ggtcctgctg gctcaggcct tgttcggtaa cccggatatc ctgatcctgg
     2461 atgagccgac caacgacctt gacgtagaaa cgatcaactg gctggagaat ttcctggccg
     2521 attatgccaa tatcgtgatc gtggtctcgc acgaccggca cttcctcgat gcagtgtgca
     2581 cccacgtggc cgatgtggac cgtcagaaga tcaagatctt taccggcaac tatactttct
     2641 ggtacgagtc ttcccagctg atggcgcgtc agctgtcgga caagaacaag aagacggaag
     2701 acaaacgagc cgcattgctc gagttcatcg cgcgtttctc cgccaatgcc agcaaatcca
     2761 aacaagccac ctcacggaaa aaggccctcg aaaagctgac gatcgaggag atagagccat
     2821 ccaaccgccg ctaccccggt atcatcttta aacaactccg agaggtcggc aaccagatcc
     2881 tcaatatcga gaagctgtcg aaaaaggcgg aaggcggcca tacccttttt tccaacgtca
     2941 gcttcagcgt caataagggg gataagatcg cttttgtcag caaggaccac ctggcattaa
     3001 caagcttttt cgacatcatc aacggacagc tgcacgccga cagcggaaag ctcgagtggg
     3061 ggactacggt caccaaggcc tatctgccaa atgacaatac ggaatatttc gagggacagc
     3121 atatcaacct gatggattgg ctaagacaat tcgcgccccc ccatgtgacc gatgtggacg
     3181 aacccttcct gcgtggcttt ttgggtaaga tgctctttag cggggatgag gtgttgaaga
     3241 acaccaatgt tctttccggt ggtgaaaaag tacgttgtat gctttcccgc atgatgttgc
     3301 aggacccgaa tgtcgtcatc ctggacgagc ctaccaacca tctcgacctg gagtccatcc
     3361 aggccttcaa cgaaagcatg agcaacttca aaggcatcgt gctgctgacg tctcatgacc
     3421 atacctttct tcagaccgta gccaaccggg tcatcgagct gacgccgtcg gggatcatcg
     3481 accggctgat gtccttcgat gaattcctgg aggatgcacg cgtgaaacag ctgcgcgaag
     3541 aaatgtatcc tcgcgaggag ctcgtcaagg catgatgaga cctttgtcct tcttttcgaa
     3601 atggtagcga cgggatcgtt ttgccgggac tctcggactt gtcatccgga acacgacacc
     3661 cagtcgtccg gtcattgcat gtgtggcggc gtccatctgg aaatagctgg tgatacgccc
     3721 gaaagtaaag tgaagcgaaa gatcgaaata agtgtatgcg cgaagctgta cgtttttgac
     3781 gctgtcgaaa atattcaggt ggttgtaggg tgatccaagg acacccccgg tggcgatacg
     3841 aaatccccat tctccatagc tggttcggcc aatgatctcg gtggtgacac ccagggtgcc
     3901 gaagaggcgg ctttttacga cctgggtgac cagcgaatcg ggcaactgtt gccgaaagga
     3961 gagataatct ccgaattcgc ggtgaagaga ggtttccaga cccaggaaac gccactcgcc
     4021 tccgcggccg agaggcatga ccgcattgat cccgccacta aaggaggtgc tgccatagaa
     4081 cttatgaccc gcgaaagccg ccagttgccg gcttccgctg ggaggtagcc aataacgggt
     4141 gctgagaagg cccccgaagg tgcgtgaggt atcccatttc ccgacgatat aagtgccaag
     4201 cgacaggtct aatccataat agcattggaa cctgccgaaa tgatgggccg cgtatccgga
     4261 gaaactccaa ccgtcgagat agtcattgtg acgcgtgttg gcgctacccc aataaaacgc
     4321 agtcctggca tagatagcca tgggagcgct gtcctttata aggggtaggg tatggtagtc
     4381 ttcggaattg ccattgagcg ggctgacata aaaggcctgt tcgcggacag gtatgcagcc
     4441 ggaaaacgcc acggcggcgg caagacatcg gataggcagg aaataggttc ttttcggcat
     4501 atagaccaga cagggtaaaa ctatgacaaa accttgccgg tcaacgtggc atggggggcc
     4561 ggttttgtcc tatcgtatac aataaattcg ctttttaagc attttagatt aaacccgcgg
     4621 ttgttgatca ggaggctgct tatatccata ctgcttttta gtggggccag cctctccaca
     4681 agggctcagc tggccacatc cgacagtctg cgccgcatga ccgattctct ccgcagaacc
     4741 gattccctgc agcgcgccac cgacagcctg catcgcgccg attccctgca tcgcgccgac
     4801 tcgctcagaa aggtccggtc ggacacgctg ctgtcggcta cctccatgct tcaccagata
     4861 gattcgctca acgccgcccc ggcggcgccc attgattcgg tgaaactgcg tttcgatgcg
     4921 atgatgaacg gtggcgccgc ccggccgggc gatgcacccg gcacggcagt gagctccggc
     4981 gcgggaccac cccgatccgc ccctccgggt cctccgcgat cggggccgcc caccacccga
     5041 aagtcaggtc ccgccaaacc cgtgatcctc tccaatctgc accggaaata tattcccgtc
     5101 cggcccggcc gcgtccctat cgattcgatg agcctgatac cgggctcctt tctgatgaag
     5161 ggggtcggcg acagcgccta tacgctggat tgggtcaacg gcgtggtgtt atggatacat
     5221 cctccggcag cgacggacag cgtgctggtc acataccgaa gctttcccta caggatgaat
     5281 gccgttgccc ggcgattcag ctatgacagt atcgaaagct atatgctggt gcgaccctat
     5341 gataagggca aggacgatgt cgccgacaac tttttcaact ttggcaacat gacctacaat
     5401 ggcagctttg gccggagcat taccttcggg aacagccagg atgccgtggt cacgtcgaac
     5461 ctcaaccttc agatcagcgg ctatctggcc gacagtatcc agatctcggc ggccatcacc
     5521 gataataata tacccatcca gcccgacgga acgacggcca atctcaacga attcgaccag
     5581 gtcttcctgc agtttaagaa aaagaactgg gcgctgacca tgggggacat cgatcttaga
     5641 caaaatcagc tctatttcct gaatttttac aaacgtctcc agggggccag tttcgaagcg
     5701 acggaacaac tgggtccccg atctacgaat aaaaccctgg taagcgcagc catcgccaaa
     5761 ggcaagttcg cccgcaacat cttccagggt caggaaggta accagggacc ctacctgctg
     5821 catggcgata acaatgagct ttatttcatc gttctggccg ggacggagaa ggtctttatc
     5881 gacgggcagc tcatgcaacg cggagaagac caggactata ccatcaacta caataccgcc
     5941 gagatcacct ttacgcccaa ccgcctgatc agccaggacg cccgtatcca ggtggaattc
     6001 gaatatgccg acaggaatta tctgaacgtc aacctgtatg cctatgatga agccgtcatt
     6061 gcaggtaagc tgaagctccg gctcggcctt ttcagcaaca gcgatgcgcg taattctccc
     6121 attaatcaat cgttgacgcc ggatgagaac aagttccttg cgcggctggg gaacgatatc
     6181 cagcacgctt attatcccgt ggcgaatgtc gacacctttg cggcgggtaa ggtcctgtat
     6241 aaaaaggtcg atacgaccta caagaacgcg gccggcgtcg tagtacacga ttcggttttc
     6301 gtcttctcca cggacgcctc gcaaaccctc tacaacctgt cctttgccaa tgtcggccct
     6361 ggctatggca attatatacc ggacctcagc ggtatcaacg gggccgttta cgattgggtg
     6421 ccaccggtga acggggttcc gcaaggtact ttcgaggcgg ccgaatacct ggtcacaccc
     6481 aagacacagc agatagtgag cctcggggcg gactataaca tcaacaaaca tacgacggtg
     6541 acgacggagc tggcgcatag ccattatgac atcaataccc tgtctaccct tggcaaatac
     6601 aatgacgacg gcaacgccgc gaaagttacc ttcaggaatg taaagacgct gggctccgcg
     6661 gcaaagggtc tccagctgac ctcgacggcg ggtttcgagt atgtccaggc gaattttcaa
     6721 cccgtccaga gattgcgcga tccggagttc gatcgcaact ggggcatgtc gcttagcgtc
     6781 aatcccgccg atgaaaagat cttcaatgcc ggtgttcagc tgagtgataa aaaactgaat
     6841 gccgtcaaat acgagatatc caggtacgaa agaagcgacg gttttacggg gatgcagaac
     6901 aatatcagca gccggcagac ggtcctgggt tggaagatca tcgatcagct gagtctcacc
     6961 acctatgaca gcagtcaccg gttcactggc tattacctgc ggccgtcggt ggaagtttcc
     7021 cgtaagctgt cctttttgaa ggactatgtc atcagcgggg gatattcgct ggaggataat
     7081 aacaacaaag acaggtcgac cgatacggtt tcggctacga gttatgcttt tcatacctgg
     7141 accgcgagtc taaagtcacc ggagaagtta cctaacaaat gggggatggt ctatagcgag
     7201 cggacgaatt cttatcccgg cggtccgtcg ctgctggtgg gcgaccgcag taagacgttc
     7261 aatgtcttcg cagatctttt gaaaaacccc catcatcaat tccatgtcaa cgccacttac
     7321 cggcagatga ctatcgtaga cacttcgctg gccttgcata ccgttgacag tgctaccgcc
     7381 ttccggggcg gcgacaagac cctggtcgga cgggtggaat atgtcgtcaa cggctggaag
     7441 gggctgctgc ggggtaatct tctctatgaa gtgggttccg gtcagcagca gaagctggct
     7501 ttcacctatc tgcaggtgcc ggcgggcacc gggcaatatg cgtggatcga ctacaacgcg
     7561 gacggaatcc agcagctggg tgaattcgtc cttgcccagt tccaggacca ggcccagtat
     7621 atacgggtgt atacgccgac cgccgagtat atcaaggcca actacaatac gttcaactat
     7681 agtttttcgc tgaat
//
LOCUS       DASYSV010000207         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_808724, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000207 DASYSV010000000
VERSION     DASYSV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808724"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /locus_tag="VGQ51_06385"
     CDS             <1..1876
                     /locus_tag="VGQ51_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV9036224.1"
                     /translation="PAMEEYLAYVGSRSERERMAEKKITGRFTGSYAIHPFTGLRIPI
                     WTSEYVLAGYGTGAIMAVPCGDERDFKFARHFHLPVTNIIGDHFDGEEANPTKDAVLQ
                     NSGFLDGLVMKDAILIAIHHLEERGIGIRKVNYRMRDAAFSRQRYWGEPFPIIWTDGI
                     AEPLTEKDIPLELPHIEKYGPGPDGEGPLANIPAWTNRRLETNTMPGYAGSSWYFLRY
                     MDPHNEKEFCSRAASDYWGQVDLYIGGTEHAVGHLLYSRMWNKFLFDRGLIGHDEPYK
                     RLVNQGMIQGSSRFVHRLEIKANLTEPGDLGSTVPIFVSAGIAAIQGTPEFHQLINLA
                     VRDILGDATPGFDQITLSPIHVDVNIVDGVELDIEAFRKWRTEYANAQFILEEGKYIC
                     GTEVEKMSKRLYNTVNPNDLVAKYGADTFRMYEMFLGPVEQSKPWDTKGIEGVHRFLR
                     KLWRLFFDETKGAVYTNATPTEAEWKCIYRTIKKVEDATERFSFNTGVSALMIGVNDL
                     TDLKCHKAAVLEKLIVILAPYAPHVAEELSHALGHQGSVLDAAYPAVEEKYLVESAKN
                     YPIAINGKTRTELNIALDATQQQVEELVLADDIVKKWLDGHRPKKVIYVKNKMINVVV
                     "
     gene            1873..2640
                     /locus_tag="VGQ51_06390"
     CDS             1873..2640
                     /locus_tag="VGQ51_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEV9036225.1"
                     /translation="MTPLATILDRHRGDYHRVVPFDRERDRLYVFDFTESNTDLSADD
                     IANTERFATWINRTLREQKARYGFGGYDEHRTLYARSRHFDQPLAGSRLRSGSESPRV
                     LAEEEEPRRLHLGIDIWGPSGTPVMAPIDGIVHSFAFNNNDSDYGATLILTHNLEGAG
                     FHTLYGHLSLNSLKNLYEGQHVAGGEVIAEFGMRFENGNWPPHLHFQCIADMQGWKGD
                     YPGVCRFSERQQWLGNCPDPDLILGMRAAAVDMPTAT"
     gene            2761..>3871
                     /locus_tag="VGQ51_06395"
     CDS             2761..>3871
                     /locus_tag="VGQ51_06395"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9036226.1"
                     /translation="MIKYDAIIIGSGQAANPLASKLAAAGWKTALIEKKFIGGTCLNT
                     GCTPTKTLIASGRVAYLARRSPDFGIHTGEVTVDIEAVIRRKNAKVLAARESSSKHLL
                     ETHNLDIHFGTAFFSGPKEVTVLRDDGSNGTMTADHIFINTGTRPVIPTIPGLGTIPY
                     LTSDTILDLMVIPSHLAIIGGSYIALEFGQLYRRLGSDVTILENNPRFLSKEDEDIAG
                     ELRKILEEDGIRILTGATVEKITQSEGGSRLTIKNGLHHEFTASHILVATGRLPETAT
                     LHTAAAGIDLDPHGFIKVNQRLETNIPGVYALGDVKGGPQFTHISYNDHLIVYKNLVE
                     KADCSIAGRPLIYCLFTDPELGRIGITENEAREKGL"
BASE COUNT          952 a         1217 c          976 g          726 t
ORIGIN      
        1 cccggcgatg gaagaatacc tggcctatgt cggcagccgt agcgaacgcg aacggatggc
       61 ggaaaaaaag atcaccgggc gtttcaccgg cagctacgcc atccatcctt ttacaggtct
      121 gcggataccc atctggacat ccgagtatgt attggccgga tatggcacag gcgccatcat
      181 ggccgtaccc tgcggcgacg agcgcgactt caaattcgcc cgtcatttcc atctgccggt
      241 aacgaatatc atcggcgacc actttgacgg cgaggaagcg aatcccacca aagatgccgt
      301 cctccaaaac agcgggttcc tcgacggcct ggtcatgaag gacgcgatcc tcatcgcgat
      361 ccatcacctc gaagaacgcg gcattggcat ccgtaaggtc aactaccgca tgcgcgacgc
      421 cgctttcagc cgccaacggt attggggcga acccttcccg atcatctgga cagacggtat
      481 cgcagaaccg ctcaccgaaa aagatattcc cctcgaactc ccgcatatcg aaaaatatgg
      541 ccccggcccc gacggcgaag gcccgctggc gaatatccct gcctggacca accgccggct
      601 cgagaccaat acaatgcccg ggtatgccgg tagcagctgg tatttcctgc ggtatatgga
      661 tccgcacaat gaaaaggaat tctgtagccg ggccgcatcc gattactggg gccaggtaga
      721 cctctatatc ggcggtaccg aacacgccgt gggccacctg ctctatagcc ggatgtggaa
      781 caaattcctg ttcgaccggg gcctcatcgg ccatgacgaa ccctacaaac ggctggtcaa
      841 tcagggcatg atacagggaa gcagccgctt cgtccaccgg ctggagatca aagctaacct
      901 caccgaaccg ggcgacctcg ggtcaaccgt tccgatattc gtttcggccg gcatcgctgc
      961 catccagggc acacccgaat tccaccagct gatcaatctg gccgtacgcg atatcctcgg
     1021 cgatgcaacg cccgggttcg atcagatcac cctctccccg atccatgtag acgtcaacat
     1081 cgtggacggc gtcgagctgg atatcgaagc tttccggaaa tggcgcacgg aatatgccaa
     1141 cgctcaattt atcctcgaag aaggcaaata catttgcggg acggaggtcg aaaagatgag
     1201 caagcggctg tacaataccg tgaaccccaa cgatctcgtc gcgaaatatg gtgccgatac
     1261 cttccgtatg tatgaaatgt tccttggccc ggttgaacaa agcaaaccat gggatacaaa
     1321 aggtatcgaa ggcgtacacc gcttcctccg caaactgtgg cggctgttct tcgacgagac
     1381 caagggcgcc gtatatacga acgccacgcc caccgaagcc gaatggaaat gtatctaccg
     1441 aacgatcaaa aaggtcgagg acgcgacgga gcgtttctcc ttcaataccg gcgtcagcgc
     1501 actgatgatc ggcgtcaacg acctcaccga ccttaaatgc cacaaagcgg cggtcctcga
     1561 aaaactgata gtcatccttg caccctatgc cccgcatgtg gcggaggaac tatcccatgc
     1621 cctcggtcac cagggctccg ttctggatgc cgcctatcct gccgtcgagg aaaaatacct
     1681 cgtcgagtcg gccaagaact atcccatcgc catcaacggt aaaacccgca ccgagctcaa
     1741 catcgcgctc gacgcaaccc aacagcaagt cgaagagctt gtccttgccg acgacatcgt
     1801 caaaaaatgg ctcgacgggc accgacccaa aaaggtcatc tacgtaaaaa acaaaatgat
     1861 caatgtagtt gtatgacgcc gctggctacc atattggaca gacacagggg cgactatcat
     1921 cgcgtggtcc ccttcgacag ggaacgtgac cgcctttacg ttttcgactt caccgaaagc
     1981 aataccgatc tgtccgccga cgacatcgcc aataccgagc ggttcgcaac ctggatcaac
     2041 cgcaccctcc gtgaacaaaa agcccgctat ggctttggtg gatacgatga acaccgtacc
     2101 ctttatgcgc gtagccggca tttcgatcag ccgctggcgg gatcccgcct gcgctccgga
     2161 agcgaatctc cgcgggtgtt ggccgaagaa gaagaacccc gccgtcttca cctgggcatc
     2221 gatatctggg gaccctccgg cacgccggtc atggcgccga tagacggcat cgttcactct
     2281 tttgccttca acaacaacga cagcgactac ggagccaccc tgatcctgac acataacctc
     2341 gaaggcgcag gtttccacac gctctacggc cacctcagcc tgaattcatt gaagaatctc
     2401 tacgagggac agcacgtcgc cggcggcgag gtcatcgccg agttcggcat gcggttcgag
     2461 aacggtaact ggcccccgca cctgcatttc cagtgtatcg ccgacatgca gggatggaaa
     2521 ggggattacc ccggcgtatg ccgtttttcc gaaagacaac aatggctcgg caattgtccc
     2581 gaccccgatc tcatcctcgg aatgagagcg gccgcggtcg acatgccgac ggcaacctga
     2641 tcctcgtcat cgtctcccag ggaagaacca gcgccgcacc gggaaccatc tccccatccc
     2701 ccccagcgac ggccggcacc gggaacaata attgcttaaa tttggacacg gacaacgcgc
     2761 atgatcaaat atgatgctat catcatcggc tcaggccagg cagctaaccc cctggcatcg
     2821 aaactggctg ccgccggatg gaaaacggca ttgatcgaaa agaaattcat cggcggtact
     2881 tgtctcaata ccggctgcac accaaccaaa acactcatcg cttccggccg tgtcgcttac
     2941 ctggccagac gaagccccga tttcgggatc cataccggtg aagtcaccgt ggacatcgaa
     3001 gctgtcatcc gccgaaagaa tgcaaaagtg ctcgctgcac gtgaatcctc ctccaaacat
     3061 ctcctcgaga cacataatct cgacatacat ttcggcactg cctttttctc aggacctaaa
     3121 gaggtgaccg tgctgagaga tgacggttcc aacggaacca tgaccgccga tcacatcttc
     3181 atcaataccg gtacgagacc cgtcataccg acgattcccg gactcgggac cattccctat
     3241 ctgacctcag acacgatcct cgacctgatg gtgatcccct cccatctggc tatcatcggc
     3301 ggcagctata tcgcgctcga gttcggacag ctttatcgtc gattgggcag cgacgtaacg
     3361 atcctcgaaa acaacccccg gttcctctcc aaagaggacg aggatattgc cggggagtta
     3421 agaaagatac tcgaagagga cggcatccgc atcctgaccg gcgccaccgt tgaaaagatc
     3481 acgcaaagcg agggcggtag ccggcttacc atcaaaaacg ggcttcacca cgaattcacg
     3541 gcctcccata tcctcgtagc taccggccgc ctgcccgaga ctgccacatt gcacactgcc
     3601 gccgccggca tcgatctcga cccgcatggc ttcatcaagg tcaaccagcg tctcgaaacc
     3661 aacatccctg gggtctatgc cctgggtgac gtcaagggcg ggcctcagtt cacgcatatc
     3721 tcttacaatg atcacctcat cgtttataag aacctggtag agaaagccga ttgcagcatc
     3781 gccggcagac cgctgatcta ttgtctgttc accgaccccg agctcggccg catcggcatc
     3841 accgagaacg aagccaggga aaaaggtctt a
//
LOCUS       DASYSV010000208         4312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2213442, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000208 DASYSV010000000
VERSION     DASYSV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213442"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VGQ51_06400"
     CDS             complement(<1..947)
                     /locus_tag="VGQ51_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036227.1"
                     /translation="MAFLFLRYGINSCHMLANYYKTAVRSIARSRFHFFINVIGLSIG
                     IAVTLLIAAYCWSETQVNRRLLNADRQFILTNQFKVPGSGFPLTTWGPLARTLKENYP
                     SLVANYFRFDGITVVVSNGDKYFREGLQVGDSTLLPMYGFSLLYGDARTALDNPFSVV
                     ITDEKAIKFFGTTDVVGKDLVIDNFSGQKKSFRITAVMKRPSRNTITQLTAAFENGIF
                     VPVSNLAYFGRNMDWPNGAIASFIELRKGVAPEELNHPIEQIIKTNLKPAIAANVRVL
                     VQPLNAYYLDANGGTVRKMLYTLSFIAVFILAMAIINFI"
     gene            complement(1046..1452)
                     /gene="rnpB"
                     /locus_tag="VGQ51_06405"
     ncRNA           complement(1046..1452)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGQ51_06405"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1535..2302
                     /locus_tag="VGQ51_06410"
     CDS             1535..2302
                     /locus_tag="VGQ51_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036228.1"
                     /translation="MKSYLLVLSILCAWLQMSAQSPKPSRWSVALTAGPAIPVGTFAG
                     THNSNAGDKTVSTGGLLELSGSYRIWRFFSAAVAFDGQLNRGNGISSDLGPMYLYNSA
                     AVAHIGVFGKDWQIARVLAGGLFTLPLSKKGGPTLMLRALAGIQKTRTPAVSYPESPV
                     ITTYPGTFYVRANSLSTAFSYQLDAGLKWKLSSKWAFLVFAGYNGCRPSFSPHLIFYP
                     PGEMVPGPSGAGPLMSNRNTSLPTGTISIRAGLSYSL"
     gene            2521..2694
                     /locus_tag="VGQ51_06415"
     CDS             2521..2694
                     /locus_tag="VGQ51_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036229.1"
                     /translation="MIGASQMRQARSSVAYAGATAGYRVSGSPDPGFPLPSTRHAVPT
                     GSLQARIGAEIGL"
     gene            complement(2695..3744)
                     /locus_tag="VGQ51_06420"
     CDS             complement(2695..3744)
                     /locus_tag="VGQ51_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEV9036230.1"
                     /translation="MKFEVGDKVVIQHSNDEGEVVEIINDKMVLVDVRGVRFPAYTDQ
                     LDFPYYKRFTEKKLFPQEKKAKKYIDQLPPEKPAAGKKKERIAQLADGVWITFLPVFD
                     NDEFGDDVVDSLKIWLVNNTSIGYRFSYQLNYFGKSEFDLDGQLPSLHDFYLHDIPFA
                     NLNDNPVFECEFSLLTPDKTRAEHYEASLKLRAKQVFGRIEDIRKKGEATFSFKLFDE
                     YPFAPVEETVDTRSLLSGSGFRIYEASKARQHLEPARQELDLHAEKLTDKAASLDNFE
                     ILTLQLQTFEKYFDLAIAHHLPSMTVIHGVGSGRLRDEIHEVLRVRRQVKSFINRYDP
                     RYGYGATEIFFQYGK"
     gene            complement(3754..>4312)
                     /locus_tag="VGQ51_06425"
     CDS             complement(3754..>4312)
                     /locus_tag="VGQ51_06425"
                     /inference="COORDINATES: protein motif:HMM:NF025274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HEV9036231.1"
                     /translation="KFHTPWFSTIITGIIVAVPSLFMRKDLMEQLTSIGTLFAFVLVS
                     GGVLMLPAITGRGRGFRMPYVSGRYIVPVLYSAFIVLFHQRVADDLQHLTTGGLQEVL
                     FLTFTFLAGVLTLATVVRKYSVIPVLGVLFCAYLLIEIPAIAWEWFFVWMGIGLMVYF
                     AYGYRKSRLTSQSGIQAKPGVRANP"
BASE COUNT          947 a         1221 c         1149 g          995 t
ORIGIN      
        1 ttgataaaat tgatgatggc catcgccagg ataaatacag cgatgaaaga aagcgtatac
       61 agcatctttc tgacggtgcc gccattggca tccaggtagt aggcgttcag cggctgtacc
      121 aggacacgga cattggcggc tatagcaggc ttgaggttcg tcttaatgat ctgttcgatt
      181 gggtggttca actcttccgg ggcgacgcct ttgcgcagct cgatgaagct ggcgatagcg
      241 ccattgggcc agtccatatt ccttccgaaa taggccaggt tcgagacggg aacgaaaata
      301 ccgttctcga aagcagcggt gagttgggtg atcgtgttcc gggagggccg tttcatgact
      361 gccgtgatcc ggaatgactt tttctgcccg gagaagttgt cgatgaccag gtctttccct
      421 accacatcgg tagttccgaa gaattttatc gctttctcat cggtgatcac gacagagaaa
      481 ggattgtcca gcgccgtgcg ggcatctcca tacagaaggg agaatccata catggggagc
      541 agcgtgctgt cccccacttg caacccctcc cgaaaatatt tatccccatt ggagaccacg
      601 actgtgatgc cgtcaaaccg gaaatagttg gccaccagcg aaggatagtt ctctttcaat
      661 gtccgggcca gcgggcccca ggtggttagc ggaaaaccgc tgccggggac cttgaattga
      721 ttggtcagga tgaattgtct gtccgcgttg agcagtcgcc ggttcacctg ggtttcactc
      781 cagcaataag ccgcgatcag cagcgtgact gcgatgccga tggaaaggcc gatgacattg
      841 atgaagaaat gaaacctgct ccgggcgatg ctgcggacgg ccgttttgta gtagttggcg
      901 agcatgtgac aggaattgat cccatagcgt agaaataaga atgccaaact cgcaataaat
      961 tggcaaccaa caagaattaa agaccgaatg gccgtgtagt gtccgtttac gcgacacgtg
     1021 atgaccgttg ccgtacaaag aaaaaaaatg ctaccaacct gtaagccgga tcctgttccc
     1081 gtcatcggct tgcgccgaac gacgggtggc catcatttat ctggcccctg cattactaca
     1141 gggatctagc tgcctaccct ccggcgtcgg ccccttgcgg gaccatcgga cgagccgccc
     1201 tcgaccaccg gtatacgtgg catttcagca tgcaaggttt acccgcgccg gcggttaccc
     1261 gccggcgccg tgggctctta ccccacgttt tcaacctcac cgcgcgacag cctgagccac
     1321 cgcatggcag ttattttctg tggcactttc tgtgaacgct tgcgcgcccc ccggctcctg
     1381 accggtgcat tgccctttgc tgtccggact ttcctccccc gtgtgaacgg gagcgatgac
     1441 ccggttggta gcgtctgcga agatacaaaa agccgggcgg ccatgcaacg atcggttcgt
     1501 aagtttgatc ataagaatgc caaacttatc gccgatgaag agctatttac tggtcctgtc
     1561 catcctatgc gcatggttgc agatgtccgc gcaatcgccc aaaccttcgc gttggtctgt
     1621 cgccctgacc gccgggccgg ccatccccgt cggcactttc gccggcaccc acaattccaa
     1681 cgccggtgac aaaacggtct ctaccggagg ccttctcgaa ttatccggtt cgtaccgtat
     1741 ctggcggttc ttcagcgccg ccgttgcttt cgacggccag ctgaatcgcg gcaatggaat
     1801 atcctccgat cttgggccga tgtatctcta caacagtgca gccgttgccc acatcggagt
     1861 cttcggcaag gattggcaga tcgcgcgggt tctcgcaggc ggcctcttta cactgcccct
     1921 ttctaagaag ggcggcccca ccctgatgct gagagcactg gcgggaattc agaaaaccag
     1981 gactccggct gtctcctacc cggagtcccc cgttataaca acgtacccgg gcactttcta
     2041 tgtccgcgcg aacagtctgt cgacggcatt ctcttaccag ttggatgccg gcctcaaatg
     2101 gaagttgagc agtaaatggg cgttcctcgt ctttgccggc tacaatggat gccggccttc
     2161 cttcagtcca cacctgatat tttaccctcc cggcgaaatg gtgcccggtc ccagtggcgc
     2221 cggcccgctg atgagtaatc gcaacaccag cttacccacc ggaacgatct ccattcgtgc
     2281 aggattaagc tattcgctat gagagcgacc ctattgctgt tgcttcccct tttcttcata
     2341 gaaagcaccc acgcgcaaaa accggcagcg gcctcttccc gctggtccat cgacattatg
     2401 gcgggcccat cggtcaacaa ggtccaacca cgcttcaatg cctgccgttg tcggcgaaga
     2461 aaggccccgt cctgctgatc agggcgatgg ccggcgtcct gaaaaccagc ctgccgggtt
     2521 atgattggcg cttcgcagat gcgccaggcc aggagctcgg tcgcttatgc aggtgcgact
     2581 gccggctatc gggtttcggg ttctcccgat ccgggatttc cgcttccgtc taccagacat
     2641 gccgtcccga ccggctcgct gcaggcacgc atcggagccg aaatcggtct ttgatcattt
     2701 cccgtactgg aaaaatattt cggtggcccc atatccataa cggggatcat acctgttgat
     2761 gaacgatttt acctgacgcc tgacccgcag gacctcgtgt atctcgtcac gcagccgccc
     2821 gctgcctacg ccgtggatga ctgtcatgga gggcaaatgg tgagcgatgg ccaggtcgaa
     2881 atatttttcg aaggtttgaa gctgtaatgt cagtatctcg aaattgtcga gcgatgccgc
     2941 tttatcggtc agcttctcgg cgtgcaggtc gagttcctgg cgcgcgggtt cgaggtgttg
     3001 cctcgccttc gaggcctcat aaatccggaa gcccgatcct gacagcagac ttcgggtgtc
     3061 cacggtctct tcgacagggg caaaaggata ttcgtcgaat agcttgaacg agaaggtggc
     3121 ctctcccttt ttcctgatat cctctatacg gccgaagacc tgtttagccc ggagcttcag
     3181 cgaggcttca taatgttccg ccctggtctt atcgggcgtc agcagcgaaa attcgcattc
     3241 gaatacgggg ttgtcgttca gattggcaaa ggggatatcg tgcagataaa aatcgtgcag
     3301 cgatggcagc tggccatcga gatcgaattc gcttttgccg aaatagttca gctggtaaga
     3361 gaaccgatat ccgatagagg tattgttgac cagccagatc ttcagactgt cgacgacatc
     3421 atctccgaat tcgtcgttat cgaaaacagg gagaaaggtg atccagacac cgtcggccaa
     3481 ttgcgcgatc cgctctttct ttttcccggc ggccggtttt tccggcggaa gctgatcgat
     3541 gtattttttc gcctttttct cctgtggaaa gagttttttt tccgtgaacc gcttatagta
     3601 tgggaaatcc agttgatcgg tataagccgg gaaccgcacg ccccggacat cgaccaggac
     3661 cattttgtca ttgatgatct cgaccacttc tccctcatca ttggaatgct gaatgactac
     3721 cttatcgcct acttcgaatt tcatcctgca aaattaagga tttgcgcgga cgccgggttt
     3781 ggcttgtatg cctgattgcg aggtcagcct gctcttgcga tagccatagg caaaatagac
     3841 catcaacccg atacccatcc agacaaagaa ccattcccag gcgatggccg gtatctcgat
     3901 cagcaggtat gcacagaaca gcacgcccag caccgggatc accgaatatt tgcggaccac
     3961 cgttgcaagc gtcagcacgc ccgccagaaa ggtgaaggtc agaaaaagca cctcctgcag
     4021 cccgccggtt gttaaatgct gcagatcatc cgctaccctt tggtggaaaa gaacaataaa
     4081 tgcgctatat agaacgggta cgatataccg gccgctgaca tatggcatcc tgaatcctct
     4141 cccccgccct gttattgcag ggagcatgag caccccgccg gataccagca caaaggcaaa
     4201 aagcgtcccg atactggtca gctgctccat caggtccttc cgcataaaga gcgatggcac
     4261 ggcgacgatg atacccgtga tgatagttga aaaccagggc gtgtggaatt tc
//
LOCUS       DASYSV010000209        11006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_675919, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000209 DASYSV010000000
VERSION     DASYSV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11006
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675919"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VGQ51_06430"
     CDS             complement(<1..181)
                     /locus_tag="VGQ51_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV9036232.1"
                     /translation="MDSTLFTATVLANWETAINRATVFFDKCSDADLLKPIAPGKNRV
                     IYLLGHLTAVSDRMLP"
     gene            complement(214..648)
                     /locus_tag="VGQ51_06435"
     CDS             complement(214..648)
                     /locus_tag="VGQ51_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9036233.1"
                     /translation="MSRVSIYLNFQGNTEEAFNFYKGIFGTEFSGPIARMNDVPMQPG
                     MPPLPEKEKNAVMHVDLPIIGGTHLMGTDMLESMGHKLKVGNNTTICLEPDSREETDK
                     LFKGLSAGGSEIEVPKDMFWGSYWGCCLDKYGVRWMFNYMKR"
     gene            complement(711..2042)
                     /locus_tag="VGQ51_06440"
     CDS             complement(711..2042)
                     /locus_tag="VGQ51_06440"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV9036234.1"
                     /translation="MTKTLLLLLLSSLAVSGSLKAQSANFNFSNPAAPVSGWTNVVGD
                     PYLSVVTATSNGITISSVATANWAQYNGNSAFNGLGESTGNSVFPGGVLLNHWYQYSA
                     PGEYTAGKNQFLISGLNPNNVYTIDMAGSSTSSLNNNPVQYTVSGIIVYPVQQENNHN
                     NSTNYASFNVAPDASGNVKVYVNTLPTTDIGDISGITITDNGAAPAPTASITSPTNGA
                     TLTQGNITISANAAVSGGGTISSVQFYAGATLIGTSSTSPYSVTWANAAPGTYSLTAK
                     ATDYLNRTVTSSAVSVTIQAAGGGGGSWIFSSGTLYDATDNVAIGTNSAQGYKFAVNG
                     TAIFTKVLVKPFPNWPDYVFRKDYVLPGLDAVEAYIHAHHHLPGIPSEKEVSGGVDVG
                     AQQTALLKKMEELTLYLIRENKSLREQNARLEAQQKEIDELRAMIQANGKH"
     gene            2348..4777
                     /locus_tag="VGQ51_06445"
     CDS             2348..4777
                     /locus_tag="VGQ51_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036235.1"
                     /translation="MQSPGLKLALRQFQKNRSFSLLNLLGLTLGLTTFLLIVLYVTDE
                     LSYDHYNINSGRIVRIHTDICLNGQVTSFADASPAIAAQLTQSYPEVEKAVRILPEED
                     HLFRKDKEDILEKRLAFVDPGFFQVFTITTIEGDPTAALLDPHSAILTSSTAKRWFNT
                     THAIGRTLIRAGDTIAYTVKAVIADMPAQSHFHYDILLSMLGNGMRKNHNFYAIYRMS
                     TYVLLKPGADKTVFDAKLSKFMRRFAPEYAAMEDDNHGSYYVHLSAMPVTDIHLHSNR
                     SDELEPGGSIQYVRIFSAVAVLVLLIAGINFMNLATARSVSRAREIGVRKVLGSARRA
                     LIAQFLGESVGMTLAAAILALILTILLLPRFNELTGKQLSVTVATLRWLLPALTAIAA
                     IVGLGSGIWPAFFLSGFRPVEVLKGKASPGGKAGGFRNALVVVQFTISVFLIVGTLVV
                     FRQLNYIRNKDLGFDRSQVLIVKNLDVLAGPPGAPAVAPGLGYLKNEVRSWPGVTDAT
                     ITSFLPTGQRRWHNWGVRKGDPNALQTELWEVDEGYIPTMDMRLLLGRNFSRQYPTDS
                     TAIILNETAARRFGIADDPLGKTIRYDGYWHGPINSTVIGVIKDFNFNSIRSAVTPLV
                     LIDRPDYAPDLAVRIQPGQIPQILKNIKAQWPALGTHRPFEYSFMDEDFDAIYRAEQR
                     MGRVVTVLSTLAILIACLGLFGLSAHAAEQRTKEIGIRKVLGADTKGIVALLSKDFAR
                     LIAIAIGIAVPLSWLASWRWLQDFAYRTTIGPRPFIVAASIVVILALLTTIFQSLKAA
                     VINPAHTLRSE"
     gene            complement(5006..7381)
                     /locus_tag="VGQ51_06450"
     CDS             complement(5006..7381)
                     /locus_tag="VGQ51_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV9036236.1"
                     /translation="MLTIKSHKRIALFFLSILLLTAPAMATVKSWQKETDGVSFVLDV
                     GEMKIRVCRPDIIEVQYSILPRLPQRRSLVITNLFSEAASFTVSETGSTVVIVTKQLR
                     ITIDKSSNAITYSDPGGKTILAEDAKDNKSMERSVLATSISTYSCSTGFQSPADEGLF
                     GLGCHPLDSGSIDYKGRDQEMLIKYMTGAIPVMLSTKGYGLLWDNYSASKFYGSDPAK
                     YRYVSESGNLVDYYFFYGPGFDHIISSYRDATGRAPMFGRWAFGLFQSEDRYKSQAEV
                     LGAGAGYRKAHIPVDVIVQDWYYWEPLPIGSHVMNPQRYPDPKGMLDSLHDEHLHGMI
                     SIWPVFGKGTANYDALKAMGGLTSITWDNVVTHTFDTYYDAHNPDARQLYWEQARDSL
                     VKRYGWDAWWIDQCEPDNGTLLDARRQAQFFTGRGVDFFNTYSLMHTDGIYKGWRKDI
                     PGKRAFFLARQAFAGQQRNGATLWSSDIQCTWHDFKDQIPQGINACAAGIPYWTSDIG
                     GYHLNWQAPDWSTPDNRELFTRWFQFGTFCPIFRIHGKGQRALYSRNWDEKTRATLLD
                     YDKLRYRLLPYIYSLAGMVTQDNYTIMRSLAFDFRDDASVYGVADQYLFGPAFMVCPV
                     TTDGARTRPVYLPKAAWYDFWTGARVDGGKTVQAAAPVDKLPLYVRAGSIVPMGPEME
                     YAMQRPEDMVELRIYPGADGQFTLYEDAGDGYEYEQGAFATTKFSWKDKLRTLAISDT
                     KGDFPGRLKKRVFNVVIVGEGNGVGEGGGAKVAKKVVYLGKAVSVVVPQNR"
     gene            complement(7508..9022)
                     /locus_tag="VGQ51_06455"
     CDS             complement(7508..9022)
                     /locus_tag="VGQ51_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036237.1"
                     /translation="MAKAKKTAVKAPVGATAVGKATGKVTSIRVRMYRGGTGDFFLLQ
                     FVAGTTVTFRMMIDCGCIRGSKEVFTPLIADLVKETKGKIDLLVVTHEHADHINGFQL
                     MSKEFEKIEVAKLWFAWTEDDTDKLANEYRKKYTRMKMALAAAGMKLRALSNDTKYKQ
                     QFGREQQFAGMLAAQQDFADRIDELYALNALAATGSGTDTMVDKLKEWKVIGKDTVVE
                     FFSPGDIIENLQGATGLRFYVLGPPRELSYLSMEQKTGQTYEKREEKSTVEFAFVTAL
                     ADGPNRDAAHSPFDGAYELKKDAGSPIRASYQAKENGWRTVDNDWLNSAGALALELQQ
                     SINNTSLALAIQFEDSERVLLFPGDAEYGCWQSWHDKLRWNVRIGGKQRIVDINYLLK
                     NTVFYKVSHHLSQNGTASHLGVDLMTHPDLAAMATLDLKRIMDLWRNTMPNDFLGAQL
                     ITRTRGRIFFTGEREPILKNIRTGRVTVGKSAVEMVDGYNKPFDRSIFIEYEVK"
     gene            complement(9026..>11006)
                     /locus_tag="VGQ51_06460"
     CDS             complement(9026..>11006)
                     /locus_tag="VGQ51_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036238.1"
                     /translation="IGAGGAAGTATGGAADDDGFREGPTGEYLEVVDYDPTVQKFYAP
                     VNLNDPYILAQDGLAPSESNPQFHQQMVYAVAMTTIKNFEMALGRPIMWAPHVWIDKQ
                     QGKSVTHYDFVRRLRIYPHAFRDANAYYSPQKKALLFGYFSAQPAEITIQMPNTVVFT
                     CLSHDIIAHETTHAILDGMYRRYIDATNPDTLAFHEGFADIIALFQHFTFPEVLRHQI
                     ARTRGDLASQNLLGELAQQFGTAIGSYGALRDAIGKIDEKTKKWVPIEPNGNEYQTTT
                     EPHARGSILVSAVFDAFLAIYKSRAADLLRIASGGTGILAQGELPTDLVNRLASEAAK
                     SARHVLGMCIRALDFCPPVDITFGDYLRGVITADADLVADDRNGYRIAFIDAFRKRGI
                     YPTGIRNLSIESLCYPHPSATDLQDIGTHLSILTTFLREFHTNTIYTTDREKLFNITG
                     DYIGGGKTDTEVKVLGLHRRINMKFLDSDKFQSLTGLVLNRHWADLGIRTSNAYNQQM
                     PSFEVHSLKVASRVGPDGNKTNQIVLTLIQRCGIRKINEKPDGPPTVETCTPAEQEKT
                     GEGFIMTGGCTLIFDLDSTQLKYAISKPLLDIDRLKAGGKDDASEGGKYGIDAARAAK
                     MDGYLNQRPTDTAFQAYFAPKPGSVVEPFALLHQS"
BASE COUNT         2408 a         3503 c         2565 g         2530 t
ORIGIN      
        1 atggcagcat cctgtccgac accgccgtca gatgccccag cagatagatc acccggttct
       61 tgccgggcgc gatcggtttc aaaagatcgg catcgctgca tttgtcgaaa aagacagtag
      121 ccctgttgat cgccgtctcc caattagcga gaacggtagc agtaaaaagt gtggaatcca
      181 tatgttgata ttaggccccg aatgtaagca tatttaccgc ttcatataat tgaacatcca
      241 cctcacccca tacttatcca aacagcatcc ccaatacgaa ccccaaaaca tatccttcgg
      301 gacctctatt tccgacccgc ccgccgacaa tcccttaaaa agcttgtccg tctcttccct
      361 gctgtccggc tccagacaaa tagtcgtatt attccccacc ttcagcttat gtcccatgga
      421 ttcgagcata tcggtgccca tcaggtgcgt cccgccgatg atcggcagat cgacatgcat
      481 aaccgcattc ttctctttct ccggcagcgg cggcattccc ggttgcatcg gcacatcatt
      541 catcctggct atcggccccg agaattcagt cccgaagatc cccttgtaaa aattgaacgc
      601 ttcctccgtg ttaccctgaa aattcaggta aattgatact cttgacatat gctttgcatt
      661 tagtgtgatt gatcctgcaa agctgccaat ttttttccaa acaccatcga tcaatgttta
      721 ccattcgcct gtatcatcgc cctcaattca tcgatctctt tttgctgcgc ctccagccgg
      781 gcattctgct ccctcagcga cttattctcg cggatcaggt agagcgtcag ctcttccatt
      841 ttcttcagca acgccgtctg ttgcgcgccc acatcgactc cgccgctgac ttccttttcc
      901 gatggaatac ccggcagatg atgatgcgca tggatatagg cctccacggc gtcgagcccg
      961 ggcagcacat aatccttcct gaacacatag tccggccagt tcgggaaggg cttaaccagc
     1021 actttcgtaa aaatagccgt tccattcacg gcgaacttat acccctgcgc gctgttcgtg
     1081 ccgatagcga cattgtccgt ggcatcatac aaagtcccgc tggaaaagat ccagctgccc
     1141 cctcctcctc ccgccgcctg gatggtgacg ctcacagccg aagatgtcac cgtcctgttc
     1201 agataatccg tggctttggc agtcagcgaa taggttcccg gcgcggcgtt tgcccaggtg
     1261 acgctgtagg gcgatgtgga cgacgtaccg atcagtgtcg cacccgcata gaattgtacc
     1321 gagcttatcg taccgccgcc gctgaccgca gcattggccg agatggtgat attgccctgc
     1381 gtcagcgtgg cgccattcgt cggactggta atactggccg tcggcgccgg ggcggcgccg
     1441 ttatccgtga tcgtaattcc gctgatgtcc ccgatatctg tcgtaggcag cgtgttgaca
     1501 tagaccttca cattcccgct ggcatccggg gccacgttga agctggcata gttggtggaa
     1561 ttattgtggt tgttctcctg ctggacggga tacacgatga tccccgacac cgtatattgg
     1621 acggggttgt tattgagcga agaggtgctg ctgccggcca tgtcgatggt atagacattg
     1681 ttggggttga gcccgctgat caggaactgg tttttgcccg cggtgtactc ccctggcgca
     1741 ctatattgat accagtggtt caatagtact ccacccggaa agacactgtt gccggtgctt
     1801 tcccccaacc cattgaaggc cgagttgcca ttgtattgcg cccagttggc cgtggccacc
     1861 gaactgatcg taatgccatt gctcgtagcc gtgaccaccg ataagtatgg atcacccacg
     1921 acattcgtcc agccggacac gggtgccgcg ggattcgaaa aattgaagtt agcagattga
     1981 gccttcaggc tgccggaaac ggcgaggctg gaaagcagca gcaataagag ggtcttggtc
     2041 atattttgaa gattgaaaac gatgagtaaa gtggagtaaa tgcagcagcg ggtatggatc
     2101 tgcaagagcg ggtacgtatc tgcaaacgcg gataaaggat cgtcatcgaa gatatcgaaa
     2161 atcaaacgtc gaccaataag tgtaaacact cacatagcta aatgctggcg cgtcgatccg
     2221 gctatatccg tgtcgatttt gccaacaacc gcgtcgattg cgcccataac cgtgctggct
     2281 gcatctccac ccctgccccc aaaaaagttt tccacatttt ctttaacttt ataaaaaccg
     2341 ccattcgatg caaagccccg gtctgaaact agccctccgc cagtttcaaa aaaacagatc
     2401 cttctccctc ctcaacctcc tcggtctcac gctgggcctt actacatttc ttctcatcgt
     2461 cctctatgtc accgacgagt tgtcctacga ccattataac atcaactcgg gtcgcatcgt
     2521 ccgcatccat accgatatct gtctcaacgg ccaggtcacc tccttcgccg atgcctcgcc
     2581 tgcaatagcg gcgcagctga cacaatccta tcccgaagtc gaaaaggccg tccgcatcct
     2641 tcccgaagaa gaccacctct ttcgaaagga caaagaagat atcctcgaaa aacgccttgc
     2701 attcgtcgac ccgggcttct tccaggtgtt cacgataacc accatcgaag gcgaccccac
     2761 cgccgccctc ctcgatcccc actccgccat cctcacatcc tccaccgcca aacgctggtt
     2821 caacactacc cacgccatcg gccgcacgct catccgggcc ggcgacacca tcgcctatac
     2881 cgtaaaagcc gtcatcgccg acatgccggc gcagtcgcat tttcactacg acatcctcct
     2941 ctccatgctg ggcaacggca tgcggaagaa tcataatttc tatgctatct acaggatgag
     3001 cacgtacgtc ctgctgaaac ctggcgccga caagaccgtc ttcgacgcca aactgtcgaa
     3061 attcatgcgc cgtttcgccc cggaatatgc ggcgatggaa gacgacaacc acggcagcta
     3121 ctatgtacat ctcagcgcga tgccggtcac cgatatccac ctgcattcca atcgctccga
     3181 tgagttggag cctggcggaa gcatccagta tgtcaggatc ttttcggccg tcgcggtact
     3241 cgtgctgctg atcgcgggca ttaatttcat gaacctcgca acggcaagat cggtcagtcg
     3301 tgcccgtgag ataggcgtcc gtaaagtatt ggggtccgcg cggcgcgcgc ttatagctca
     3361 gttcctcggc gagtctgtag ggatgacgct ggccgccgcc atcctggctt tgatactaac
     3421 catcctgcta ctaccccggt tcaatgagct caccggcaaa caactgtccg tcacagtcgc
     3481 cacccttcgc tggttactgc cggcattaac ggccatcgcg gcaatagtag gccttgggtc
     3541 cggcatctgg ccggccttct tcctttccgg cttccggccc gtcgaagtcc tcaaaggcaa
     3601 agcgtctccc ggcggcaaag ccggcggttt tcgcaacgca ctcgtcgtcg tccagttcac
     3661 catatcggta ttcctcatcg tcggcaccct ggtcgtcttc cgccagctga actacatccg
     3721 aaacaaggac ctcgggttcg atcgcagcca ggtactcatc gtaaaaaacc tggatgtatt
     3781 ggctggccct cccggcgccc ccgccgtagc cccgggtctg gggtatctga agaatgaagt
     3841 ccgcagctgg cccggcgtaa ccgacgccac gatcacgtcc tttctcccta ccggccagcg
     3901 ccgctggcac aactggggcg tgcggaaagg tgatcccaac gctctgcaaa ctgaattatg
     3961 ggaagtcgat gaaggctata taccgacgat ggatatgcgg ctgctgctcg ggcgcaactt
     4021 ttcccggcaa tatccaaccg acagcaccgc tatcatcctc aacgagacgg cggcccgacg
     4081 cttcggcatc gcagacgatc cgctgggcaa gaccatccgg tacgatggct attggcacgg
     4141 cccgataaac tccaccgtca tcggtgttat aaaagatttc aatttcaact ccatacgttc
     4201 cgccgtcacc ccgctggtgc tcatcgatcg ccccgactat gcccccgatc tcgccgtccg
     4261 catccaaccc ggccaaatcc cgcagatcct caaaaacatc aaagcccaat ggccggcact
     4321 cggtacccac cgccccttcg aatactcatt tatggatgaa gacttcgacg ccatctatcg
     4381 cgccgaacaa cgcatgggtc gtgtcgtaac cgtactgtcc acccttgcca tcctcatcgc
     4441 ctgtctcggt cttttcggtc tatccgccca tgccgccgaa caacgcacca aagagatcgg
     4501 catccgtaag gtcctcggcg ccgacacaaa aggcatcgtc gcgctcctct caaaagactt
     4561 cgcgcgtctc atcgcgatcg ccatcggcat cgcggtcccg ctttcctggc tcgcctcctg
     4621 gcgctggctg caggatttcg cctatcgcac caccatcgga cccagaccct tcatagtggc
     4681 cgcgtccatc gtcgtcatcc tcgcattgct caccactatc ttccaatctc taaaagccgc
     4741 cgtcatcaac cccgcccata ccctacgctc cgagtaacgg gctgtcagcc gcgacaaagc
     4801 cggccgcgac cctattcctt taccactttc accgtacccc tcacctgccc cccacggaca
     4861 acaggtacat cccttgcgca atccgatcca ttcccggtaa cacgatcgta ttcgcaccgg
     4921 gtcccacccc gcgagtcatc ccaagtcatc cccagtcatc cccagtcatc ccaagtcatc
     4981 cccagtcatc cacagtcatc cccaatcatc gattctgcgg caccactaca ctcaccgcct
     5041 tccctaaata taccaccttt ttcgccacct tcgcaccacc accctctcca acaccattcc
     5101 cctccccgac tataacgaca ttgaataccc gcttcttcaa cctccccggg aaatccccct
     5161 tcgtatccga gatcgcgagc gtccgcagct tatccttcca cgaaaacttc gtcgtcgcaa
     5221 acgccccctg ctcatactca tatccatcgc ctgcatcctc atacaaggtg aactgtccat
     5281 ccgctcccgg ataaatcctt aactccacca tatcctccgg ccgctgcatc gcatactcca
     5341 tctctggacc catagggacg atcgaacccg cacgcacata taatggcaac ttgtcgaccg
     5401 gcgccgccgc ctgcacagtc ttcccaccgt ctacacgggc gcctgtccag aagtcatacc
     5461 acgcagcctt cggcagatac accgggcgcg tgcgggcacc atccgtcgtc accggacaaa
     5521 ccataaatgc aggcccgaac agatactggt ccgctacccc atataccgat gcatcatccc
     5581 tgaaatcgaa tgccagcgat cgcatgatcg tataattatc ctgcgtcacc attcccgcca
     5641 gcgaatagat ataaggtagc agcctataac gcagcttgtc ataatccaac aatgtcgccc
     5701 gcgtcttctc atcccaatta cgcgaataca acgcccgctg acccttccca tggatcctga
     5761 aaattgggca gaaggtcccg aactggaacc aacgcgtgaa cagctcgcgg ttgtccggcg
     5821 tcgaccagtc cggcgcctgc cagttcagat gatacccgcc gatatccgat gtccagtaag
     5881 gaatacccgc ggcgcacgca ttgatgccct gcggtatctg atccttgaaa tcatgccatg
     5941 tacactggat atccgaagac cataaggtcg cgccgttgcg ctgctggccg gcaaaagcct
     6001 gtctcgcgag aaagaaagcc cgcttccccg ggatgtcctt tcgccagccc ttatagatac
     6061 cgtcggtgtg catcagtgaa taggtattga agaagtcgac accgcgacct gtaaaaaact
     6121 gtgcctgccg gcgggcatcc agcagcgttc cgttatccgg ctcgcactga tcgatccacc
     6181 acgcatccca gccataacgc ttgacaagac tatctctcgc ctgctcccag tacaactgcc
     6241 gcgcatcggg attatgcgcg tcgtaataag tgtcgaacgt atgcgtcacc acgttgtccc
     6301 aggtgatact cgtcagtccc cccatcgcct tcaacgcatc gtaattggcc gtcccctttc
     6361 caaaaactgg ccatatcgat atcattccat gaagatgttc atcatgcagc gaatccagca
     6421 tacccttcgg atccggatag cgctgcggat tcatcacgtg ggaaccaatg ggcaacggtt
     6481 cccagtaata ccagtcctgt acgatcacat ccaccggaat atgcgccttc ctgtatcccg
     6541 cacccgcacc cagcacttca gcttgtgact tatacctgtc ctccgactgg aataacccga
     6601 aagcccatct gccgaacatc ggcgcccggc cggtggcatc ccggtaggag gagatgatgt
     6661 ggtcgaaccc gggaccgtag aaaaaataat agtccaccag gttgccgctc tccgacacat
     6721 agcggtactt cgcgggatcc gatccataaa atttcgaagc cgaatagtta tcccacaaca
     6781 acccataccc cttcgtggac agcattaccg ggatcgctcc ggtcatatac ttgatcagca
     6841 tctcctggtc acggccctta tagtcgatcg accccgaatc cagcgggtga caacccagcc
     6901 cgaacagacc ctcgtccgcc ggcgactgaa aacccgtcga acaggaataa gtggaaatac
     6961 tcgtcgccaa taccgatcgc tccatcgact tgttatcctt cgcgtcttcg gccagtatcg
     7021 tcttgcctcc cggatccgaa tacgtaatcg cattgctgga cttatcaatg gtaatgcgca
     7081 gctgctttgt gacgatcacc acagtacttc ccgtctccga caccgtgaaa gacgccgcct
     7141 cgctgaaaag attggtgatc accagcgagc gtcgctgcgg cagccgcggc aaaatggaat
     7201 attgtacttc gatgatatcc gggcgacata cacggatctt catttcgccg acatcgagga
     7261 caaagcttac gccgtccgtc tctttctgcc aggatttgac ggtggccata gcgggtgcgg
     7321 taagcaatag gatcgatagg aaaaagaggg caattctctt gtgggatttg atcgttagca
     7381 tggcctaaat ataaaacatt tccccctttc caattttgtc aaaaaggtat caaattcgat
     7441 caccccaaat ccttcatccc accacctccc gcacgcgctt cgatcatccc accacccccg
     7501 cgcccgccta tttcacttcg tactcgataa agatcgatct gtcaaacggt ttattatacc
     7561 catccaccat ttccacagcg ctctttccga ccgtcaccct gcccgtacga atatttttca
     7621 ggatgggctc ccgctccccg gtgaagaaga tacgtcccct ggtacgcgtg atcagctggg
     7681 cgcccaaaaa atcgtttggc atggtgttcc gccaaaggtc catgatgcgc ttcagatcga
     7741 gtgtagccat cgccgcaaga tcgggatgtg tcatcagatc gaccccgaga tgactcgccg
     7801 tgccgttctg tgaaagatga tggctgactt tataaaagac ggtattcttc aaaagataat
     7861 tgatatctac aattctttgt ttcccgccga tgcgaacgtt ccagcggagc ttgtcatgcc
     7921 agctttgcca gcatccatat tccgcatcgc ccggaaagag caggacccgc tcgctgtcct
     7981 cgaactgtat ggcgagcgcg aggctggtgt tgttgatgct ctgctgcagt tcgagcgcaa
     8041 gcgcgcccgc gctgttcagc caatcgttgt ccaccgttcg ccagccgttc tctttcgcct
     8101 gataggatgc gcgaatgggg ctgccggcat ctttcttcag ttcgtaagct ccgtcgaagg
     8161 ggctatgggc ggcgtctctg ttcggcccat ccgccagcgc cgtaacaaaa gcgaattcca
     8221 ccgtgctctt ctcctctctc ttttcatagg tctgacccgt cttctgttcc atgctcagat
     8281 agctcaattc acggggtgga cccagcacat aaaaccgcaa ccccgtggcg ccctgcagat
     8341 tttcaataat gtcgcccggg gaaaagaatt ccacgaccgt atccttcccg atcaccttcc
     8401 actctttcaa cttatcgacc atcgtgtccg taccgctgcc cgtggcagcc agagcgttca
     8461 gcgcatacaa ttcatcgata cggtcggcga aatcctgctg cgcggcaagc atccccgcaa
     8521 attgctgctc ccgcccgaac tgctgcttat atttcgtgtc gttcgacagc gcccttaact
     8581 tcattcccgc cgcagccagc gccattttca tccgcgtata tttcttccgg tattcattcg
     8641 ccagtttgtc ggtatcgtcc tccgtccagg cgaaccacag ttttgccacc tctatcttct
     8701 cgaattcctt gctcatcagc tggaacccgt tgatatggtc cgcatgttca tgcgtgacga
     8761 ccagcagatc gatctttccc ttcgtctcct tcacgagatc ggcgatcagc ggagtaaaca
     8821 cttcctttga accgcggata cagccgcagt cgatcatcat ccggaaggtc accgtggttc
     8881 ccgcgacgaa ttgcaacagg aaaaaatctc ccgtccctcc acgatacatc cggacgcgaa
     8941 tactcgtgac cttcccagtc gcctttccca ccgccgtcgc ccccaccggc gccttcaccg
     9001 ctgttttttt tgcctttgcc ataacttagg attgatgaag taatgcaaac ggttcgacga
     9061 cacttcccgg cttaggtgca aaatacgcct ggaacgcagt gtccgtcggc cgttgattca
     9121 gatatccatc catcttcgcc gcccgcgcag catctatccc atatttccca ccttcgctcg
     9181 catcgtcctt gccccccgcc ttcagcctgt ctatatcgag caggggtttc gagatggcat
     9241 atttcagctg cgtcgagtcg agatcgaaga tcagtgtaca gccaccggtc atgatgaaac
     9301 cttcgcccgt cttttcctgt tctgccggtg tgcatgtctc tactgtcggc ggcccatccg
     9361 gtttttcgtt gatcttccta atgccgcagc gctggatcag cgtgagaacg atctgattgg
     9421 tcttgttacc gtcgggcccc acgcgcgagg cgaccttcag cgaatgcacc tcgaaagagg
     9481 gcatctgctg gttataagcg ttggaggttc ggatacccag gtccgcccag tgacgattca
     9541 gcaccaatcc cgtcagcgac tgaaatttat cggagtcgag aaatttcata tttatccggc
     9601 ggtgaagacc cagcacctta acttccgtat cggtcttgcc cccgccaata tagtcgccgg
     9661 tgatgttgaa cagtttttcc ctgtcagtcg tataaatggt attggtatgg aactcacgca
     9721 ggaatgtcgt caggatggac agatgtgtgc cgatgtcctg cagatcggtg gcgcttggat
     9781 gcgggtaaca caggctttcg atcgaaagat tccggattcc cgtgggatag atcccgcgtt
     9841 tgcggaaggc atcgataaag gcgatacggt acccattccg gtcgtcggct accaggtcgg
     9901 catctgccgt aataacaccg cggagataat ctccaaaagt aatgtccacc ggaggacaaa
     9961 aatccagcgc ccggatacac atccccagga catgtcttgc ggatttggcc gcttcggatg
    10021 caagacggtt cacaagatcg gtcggtaact ccccttgtgc caggataccc gtcccgccgc
    10081 ttgctatcct caacaggtcg gcagcacgac tcttataaat ggcgaggaac gcatcgaaca
    10141 ccgccgatac caggatcgag ccccgcgcat gcggctccgt tgttgtctga tattcgttcc
    10201 cgttaggttc gatgggtacc cacttcttcg tcttttcgtc tatcttgccg atggcgtcgc
    10261 gaagagcgcc atagcttccg atagcagtac cgaattgctg agccagctcc cccaacaggt
    10321 tttgcgacgc caggtctccc cttgtccgcg ctatctggtg ccgcaatact tccggaaagg
    10381 taaaatgctg gaatagtgcg atgatatccg caaaaccttc atgaaaagcc agcgtatccg
    10441 ggtttgtcgc atcgatatat cgcctgtaca tgccgtctaa gatggcatgc gtcgtttcgt
    10501 gggcgatgat gtcatgactg agacaggtga agactacggt gttgggcatc tgtatggtga
    10561 tctccgccgg ctgcgccgaa aaatatccga agagcaatgc cttcttctgc gggctgtaat
    10621 aggcgttcgc atcccggaag gcatgcggat agatacgaag acgccggacg aagtcataat
    10681 gggtgacgct ctttccctgt tgcttatcta tccagacatg cggcgcccac atgatgggtc
    10741 tgccaagcgc catttcgaaa tttttgatcg tcgtcatcgc caccgcatac accatctgct
    10801 gatggaattg cggattactc tcgctgggcg ccagtccatc ctgggccagg atataggggt
    10861 cgttgagatt taccggtgca taaaatttct gtaccgtagg gtcatagtcc accacttcaa
    10921 gatattcgcc cgtcggccct tccctgaaac cgtcgtcatc cgccgcaccc cctgtcgcag
    10981 tccccgccgc acccccagcg ccaatc
//
LOCUS       DASYSV010000210         5975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_163232, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000210 DASYSV010000000
VERSION     DASYSV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163232"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2172
                     /locus_tag="VGQ51_06465"
     CDS             <1..2172
                     /locus_tag="VGQ51_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9036239.1"
                     /translation="RDYPEVEKECRFRMQGGRLFKKGNQNIYEDGVVYADSTVFDVFT
                     LPMLYGNPHTALLESHTVVITERMAKKYFDAVDVVGRTLTMNDTVLYKVTGVIRDIPD
                     QSHFHYDFFLSMTELDEAKQMDQWLSNNFNTYIVIRKDADPAKLQAKFGQLITKYIEP
                     ELKAAVNVSADEFFKAGNSVGFYLTPLTAIHLHSNKTAELGANGSITNVYIFSAIAAF
                     ILLIACVNFMNLSTARSSNRAKEVGIRKVLGSLRGNLISQFMMESVLLAFVSMLLALG
                     LAWLLLPVFNQLSDKHMVIGLFSRGWLAPSMLGLVLVVGILAGSYPAFYLSAFRPILV
                     LKGNVAAGFKTGWLRNSLVVFQFGISIFLLVGTAVIYRQLSYIHDRSIGFNRDQVLIV
                     NGTDALGNHIQDFKERLGKLSGVKGVTMTGFMPTGDWRNDDAIFLTHDLDPKKAISMQ
                     TWQVDEDYIPVLGMQMADGRNFSRDFKTDSNACIVNEATLKFMQGRKPVGSNLYELND
                     IRTKKVSEYHIIGVVKDFNFNSLREVVTPMVLYLRENRGRVALRVQASDMHPLIGQVE
                     GLWRQMAPSKPFGYAFMDEEFNNIYRAEQRQGGISLSFSLLAIFIACLGLFGLAAYAA
                     EQRTREIGIRKVLGASVSGIIGLLSRDFLLLVALAAAISFPLAAWAMHLWLNGFEYRI
                     TIGWQIFALAGVVAVAIALLTVSFQAVRAALANPVKSLRSE"
     gene            complement(2202..5447)
                     /locus_tag="VGQ51_06470"
     CDS             complement(2202..5447)
                     /locus_tag="VGQ51_06470"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV9036240.1"
                     /translation="MLRYLFLLTATAAAPTLAKAQINAALFRYPDVSATHIVFSYAND
                     LWVVPKEGGKAQHLSSPAGVESWPKFSPDGNTIAYTADYDGNQSIYSISTEGGIPSRL
                     TWQGSPERVVDWYPDGKNLLFASGRQSGRERFNQFWAIPSTGGAPTRLPLPYAEYGTL
                     SPDGKSLAFTYKSQLGRNWKRYRGGWQGQIHLFNLSNFSDQDISNTEADDELPMWHDN
                     TIFFLSDRGEEKRMNLWQYDITAQKFTQLTRFSDYDVHYPSLGPQDIVFEQGGKLYLY
                     SLTGKTYKQVPVQVVTDENALRPALENSSRLIYNSNISPDGKRALFEARGDIFSVPAE
                     NGPVIDLTRSSAVAERFPAWSPDGRHIAFWSDRSGEYELTLRDAGNAGTERKLTSYGP
                     GFRYNLFWSPDSKKIAFIDKAMKIYVYDIPTGQTSFVDQGLRMTEGALQGFSASWSPD
                     SKYLTYDRDLPNAHRAVFIYDCTEKMLHQATAGFYSCSNPVFDLTGKYLFITTNQSFR
                     PLYSDLDNTFIYPNSTRLAAIVLERSTRSPLYPKDDTVAIQKEPAAVAEHPTPPKTPP
                     ATTPKSAPGPVHIDFDDLETRMVLLPAEAGNYGNLSAVEGKLIYMQAPNTGSGQRNQS
                     LHFFDLDKRTTKTIVDALDGYTLAADNKHLLVTNGGSWYIIGADEGQKLDKKLRTGEM
                     QQWVTPREEWRQILVDAWRLERDYFYDSLMHGVDWPLVRQRYLKLVDDAATREEVDFL
                     IGEMIGELSSSHTYHGGGALETTKQAQVGYLGVDWIAAGDYYKIGKIIRGAAWDAEAR
                     SPLDQPGVNIKEGDYILSVNGIPLTTKTDPYSAFGSLAGTSVELTYNNTPSLTGAHTA
                     IVETMRSEARLRNLAWIEEKRRHVETVTKGQVGYIYVRSTGVDGQNELIRQFTAQLDK
                     KALIIDERFNDGGQIPDRFIEILNRKPLAWWATRDGAAWPWPPAGNFGPKVMLINGWS
                     GSGGDAFPDYFRKSGLGPLIGTRTWGGLIGISGVPGLIDGGTITVPTFRMYNPDGTWF
                     KEGHGVDPDIDVPENLTEGARGIDNQLETAITQILQIKGFAPPNRPTPQIR"
     gene            complement(5598..>5975)
                     /locus_tag="VGQ51_06475"
     CDS             complement(5598..>5975)
                     /locus_tag="VGQ51_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEV9036241.1"
                     /translation="LGAYHAANLAFKYPHLFHKVVGLSGRYDLTTSMEHFDDLFEGYR
                     DENVYYNMPAQFIPNLSDPCLIARLKTLEIVLVVGEKDVFLENNRILSNALWSKGIWN
                     SLHLWDGESHKARYWRHMVQLYL"
BASE COUNT         1198 a         1275 c         1993 g         1509 t
ORIGIN      
        1 cgtgactacc ctgaagtgga gaaggagtgc cgttttcgga tgcaaggggg gaggctcttc
       61 aagaaaggca atcagaacat atatgaggat ggggtggtct atgcggactc gacagttttc
      121 gatgtgttca ccctgccgat gttgtatggc aacccgcata cggctttgct ggaaagccat
      181 accgtagtga tcaccgaaag aatggcgaag aagtatttcg atgccgtgga tgtggtggga
      241 cggacgctga ccatgaacga taccgtactt tataaggtca ccggggtcat ccgcgacata
      301 cccgaccaat cgcatttcca ctatgatttt ttcctgtcga tgaccgaact ggacgaggcc
      361 aaacagatgg atcagtggtt gagcaataat ttcaacacgt atattgtaat acgaaaggac
      421 gcggacccgg cgaagctgca ggccaaattc ggacagctga tcacgaagta tatcgaaccg
      481 gagctgaagg cggcggtcaa tgtttcagcg gatgaatttt tcaaagccgg aaattcggta
      541 ggtttttatc tgacgccatt gacggccatt catcttcata gtaataagac ggcagagctt
      601 ggggcgaatg gcagtatcac gaatgtatat atcttttcgg cgattgcggc tttcattctt
      661 ttgatcgcct gtgtgaattt tatgaatttg tctacggcgc ggtcgtcgaa cagggcgaag
      721 gaagtgggta tccggaaagt gctgggttcg ctgcggggaa atctgatctc gcagttcatg
      781 atggaatcgg tgctgctcgc gttcgtctcg atgttgttgg cgttggggtt ggcctggctg
      841 ttattgccgg tgttcaatca attgtcggat aagcatatgg tgatcggtct tttttcacgt
      901 ggttggctgg cgccctcgat gctcgggttg gtgctcgtgg tgggcatcct tgcgggcagt
      961 tatccggctt tttatctttc ggctttccgt cccatcctgg tattgaaggg aaatgtcgcc
     1021 gcgggtttca agacggggtg gctgcgaaat tcgctggtcg tgtttcagtt tggaatctct
     1081 atctttttgc tggtagggac ggcggtcatc taccggcagc tgagctacat tcatgacagg
     1141 agtatcgggt tcaaccggga ccaggtgctg atcgtgaatg gcacggacgc tctcggcaat
     1201 catattcagg acttcaagga acggctgggc aagttgtccg gggtaaaagg tgtaaccatg
     1261 acgggtttta tgccgacggg tgattggcgt aatgatgacg cgatctttct gacgcatgac
     1321 ctggatccaa agaaggcgat ctcgatgcag acctggcagg tggatgagga ctatataccg
     1381 gtgttgggaa tgcagatggc ggacgggcgc aatttttcgc gagatttcaa gaccgattcg
     1441 aatgcctgta tcgtcaatga ggcgacgtta aagtttatgc aggggcgtaa gccggtgggg
     1501 tcgaatcttt atgagctgaa cgatataagg acgaaaaagg tgagtgagta tcatattatc
     1561 ggagtggtga aggatttcaa cttcaactcg ttgagggagg tggtcacgcc gatggtcctg
     1621 tatctgcggg agaacagggg gagggtcgcc ttgcgtgtgc aggcttcgga tatgcatcct
     1681 ttgatcggac aggtcgaggg tttatggcga cagatggctc cgtcgaagcc tttcggttat
     1741 gcgttcatgg atgaggaatt caataatatt tatcgtgccg agcagcggca gggagggata
     1801 tcgttgtcgt tttcgctgct tgcgatattt atagcgtgtc tcggcttgtt cgggctggct
     1861 gcctatgcgg cggaacagcg gacgagggag atcggtatac gtaaggtgct gggcgcatcg
     1921 gtgagtggga tcatagggtt gttgtcgcgg gattttttgc tgttggtggc gctcgctgcg
     1981 gcgatatcct ttcctttggc ggcgtgggcc atgcatcttt ggttgaatgg ttttgaatat
     2041 cggatcacga tcggttggca gatatttgcg ttggccggtg tggtggcggt ggcaattgcg
     2101 ctattgacgg tgagtttcca ggcggtgagg gccgcgttgg cgaatccggt gaaatcattg
     2161 cgtagtgaat gagaaaaaac cccgcgtcag cggggtttcg actatcttat ttggggagtt
     2221 gggcgattgg gaggggcgaa gccttttatc tgaagaattt gggtgatggc tgtttcgagt
     2281 tgattgtcga taccgcgggc gccttcggtg aggttttcgg ggacatcgat atccgggtct
     2341 acgccgtggc cttccttgaa ccaggtgcca tcggggttgt acatacgaaa ggtcgggacg
     2401 gtgatcgtgc cgccgtcgat gagaccgggg acgccgctga tcccgatgag gccgccccag
     2461 gtgcgggttc cgatgagggg tccgaggccg gatttacgga aatagtcggg gaaggcatct
     2521 cctccggagc cactccaccc attgatcagc atgaccttgg gaccgaaatt tcctgccggc
     2581 ggccagggcc aggcggcgcc atcgcgggtg gcccaccatg ccaggggttt gcggttgagg
     2641 atctcgatga agcggtcggg tatctgaccg ccgtcattga aacgttcgtc gatgatgagc
     2701 gcttttttat cgagctgggc cgtgaactgg cgtatcaatt cattctggcc gtcgacgcct
     2761 gtactgcgga catagatata gcctacctgt cctttggtga cggtctccac gtggcgacgt
     2821 ttttcttcta tccatgcgag gttgcgaaga cgggcttcgg aacgcatcgt ttcgacgatc
     2881 gcggtgtggg cgccggtgag ggaaggggta ttgttatacg tgagctccac ggatgtgccg
     2941 gcgaggctgc cgaaggcgga atagggatcg gttttggtcg tcagggggat cccgttgacc
     3001 gaaaggatat agtctccttc ctttatattg acgccgggct ggtcgagcgg cgagcgggct
     3061 tcagcgtccc aggctgcgcc acggatgatc ttgccgatct tatagtagtc accggcggcg
     3121 atccaatcga cgccgagata acccacctga gcctgtttgg tagtttcgag cgcgcctccg
     3181 ccgtggtaag tgtgggaaga gctaagctcg ccgatcatct ccccgatgag gaaatcgacc
     3241 tcttcgcggg tggccgcgtc gtcgacgagt ttgagatagc gttgtctgac gaggggccag
     3301 tcgacaccat gcatcagaga atcgtagaaa tagtcgcgtt cgagacgcca ggcatcgacg
     3361 aggatctgcc gccattcctc gcgtggtgtt acccattgct gcatttcgcc ggtacgcagt
     3421 ttcttatcga gcttttgacc ttcgtcggcg cctatgatat accagctgcc gccgtttgtg
     3481 acgaggaggt gtttattgtc tgccgcgagg gtatacccgt cgagggcgtc tacgatggtt
     3541 ttggtggtcc ttttatcgag atcgaagaaa tggagggatt ggtttctttg gccggagcct
     3601 gtgttcgggg cttgcatata aatgagcttt ccttcgaccg cggacaggtt gccatagttg
     3661 ccggcttcgg cgggcagcag taccatgcgg gtctcgagat cgtcgaaatc gatgtggaca
     3721 gggcctgggg cggatttagg cgttgtcgcg ggaggggtct ttggcggagt cggatgttcg
     3781 gcgacggctg ccggctcctt ctggatggcg accgtatcgt ccttgggata gagtggggaa
     3841 cgggtggatc tttcgaggac gatggctgcc agacgggtgg aattgggata gatgaaagta
     3901 ttgtcgagat cgctgtacag cgggcggaag gactggttgg tcgtaatgaa caggtatttg
     3961 ccggtgaggt cgaaaacggg attggagcaa ctatagaagc cggcggtggc ctggtggagc
     4021 attttttcgg tacagtcata gataaaaacg gcgcgatggg cgttgggcag atcgcgatcg
     4081 taggtgaggt atttactgtc cggagaccag ctggcggaga aaccctggag agcgccttct
     4141 gtcatgcgca gaccctggtc gacgaaactg gtctgaccgg tgggaatatc atagacatag
     4201 atcttcatgg ccttgtcgat gaaggcgatc tttttactat cgggggacca gaacaggttg
     4261 taacggaagc ctggaccata ggatgtcagc ttcctttcgg ttccggcgtt acctgcatcg
     4321 cggagcgtga gttcgtattc gccggagcga tcgctccaga aggctatatg gcggccgtcg
     4381 ggtgaccagg ccggaaatct ttccgccacc gctgaagatc gggtaagatc gatgacggga
     4441 ccgttttctg cggggacgct gaagatatct ccacgggctt cgaaaagggc gcgtttgcca
     4501 tcgggggaga tattgctgtt gtagatgagc ctggatgaat tctcgagagc gggtcgcaag
     4561 gcattttcat ctgtgacgac ctgtacgggg acttgtttat acgttttgcc tgttaaagaa
     4621 tagaggtata gtttgccgcc ctgttcgaag acgatgtcct gtggtccgag tgatgggtag
     4681 tgcacatcgt agtcggagaa acgggtgagc tgggtgaatt tttgggccgt gatatcgtac
     4741 tgccagaggt tcattcgttt ttcttctccg cggtctgaga ggaagaagat cgtgttgtcg
     4801 tgccacatgg gcagttcgtc gtcggcctcc gtgttggaga tatcctgatc cgaaaaattg
     4861 gagaggttga ataaatggat ctggccttgc caaccgccgc ggtagcgttt ccagttgcgg
     4921 cccagctgtg acttataggt aaatgccagg cttttgccgt cgggggagag ggtgccatat
     4981 tctgcgtagg gcaggggaag gcgggtaggg gctccgccgg tggaggggat cgcccagaac
     5041 tgattgaatc gttcgcgtcc gctttggcgg ccggaggcga agaggaggtt tttgccgtcg
     5101 ggataccagt cgaccactct ttcgggtgag ccttgccagg tgaggcggga ggggatgccc
     5161 ccttccgtag agatggaata gatggattgg ttgccgtcat aatcggccgt ataggcgatg
     5221 gtgttgccgt cgggcgaaaa tttgggccat gattcgacgc cggcggggga gctgaggtgc
     5281 tgtgctttgc cgccttcctt tgggacgacc caaagatcat tggcatagga aaagacgata
     5341 tgggttgcgg atacgtcggg gtagcggaag agagcggcgt tgatctgtgc tttggcgagg
     5401 gttggggcag ccgcggttgc cgtgaggagg aataggtatc ttagcataag tgtgcgtttt
     5461 ggggatggct aatgtacaat ggaatccgtt aatttaatgg accgttggtg tataaaattt
     5521 actattatga taagaatcgt attggctttt gtgttggtgt gcccgttggg gttgcggggg
     5581 cggtggaggt gagaaagtta tagatacagc tgtaccatat ggcgccagta gcgggcctta
     5641 tgggattcgc cgtcccatag gtggagggag ttccagatgc cttttgacca gagagcattg
     5701 ctgagaatgc ggttgttttc gaggaagacg tccttttctc cgaccaccag gacgatctcg
     5761 agggttttga gtcgggcgat gagacaggga tcggagagat tggggatgaa ttgtgcgggc
     5821 atattgtaat agacattctc atcgcggtag ccttcgaaga gatcgtcgaa gtgttccatg
     5881 gaggtagtga ggtcgtaacg gccgctaagg cctacgacct tatgaaagag gtggggatat
     5941 ttgaaggcga ggttggcggc atggtaggcg ccgag
//
LOCUS       DASYSV010000211         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2468680, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000211 DASYSV010000000
VERSION     DASYSV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468680"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2207)
                     /locus_tag="VGQ51_06480"
     CDS             complement(<1..2207)
                     /locus_tag="VGQ51_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEV9036242.1"
                     /translation="MEELLNKLAELDISLEARGSELDIYDPRQGLTQELLSVIRENKR
                     QLLALLKDTGAAAPFKPISRVATAEHYALSAAQKRLYFLYEFDRSSIAYNLPKILEIE
                     GRMDVPRLERAFRRLIARHESLRTWFDTVDEMPVQRISGEVPFAIDRFYCRPEEAAGL
                     LRTYIRPFSLESAPLMRVALIGTGQDRYLLVMDTHHIIADGASQDILTRDLMLLYTDA
                     LLPALELQFKDYAAWRQGPDQQMATDQQKDFWVEMFRKQTVPLELPADMTRPPVVGYE
                     GDSLDLSLDGEMARRMKQLAGQNGASSFMIYLAVYYIFLSRMSGQEDIVVGIPTAGRH
                     YAGLENVVGMFANTLPIRNLPAGELSFFQFLAQVRSRVLACLDHQDYPYEELIDQQKI
                     ERDASRNPLFDVLFDLTHVSPGSARFPGVISRPYRLDHAVAKLDLSLYVLEAESDVTL
                     HFEYSTNLFRRDTVERFAGYFQRIVAAVTEDPEVKLGAIELLPEVEQEELLSGFNDTS
                     REYGREETLVTLFETQAAQRPEEIAVVYEGESLTYGELNRRSNGLAQRLRGEYGVGPD
                     KVVGVMMDRSLELMVGLLGILKAGGAYLPMDPGYPAERTSYILSDSGAEVLIVDRGRE
                     GPSGYGGSIVSPRGEEETDDLPRVNGSGDLCYLIYTSGSTGNPKGVMIAHRNVVNFFA
                     GLNERLPAGEDDCLLAVTSTSFDISVLELFWTLCNGIQVVIHPSGISFSGLDR"
     gene            complement(2212..>3934)
                     /locus_tag="VGQ51_06485"
     CDS             complement(2212..>3934)
                     /locus_tag="VGQ51_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEV9036243.1"
                     /translation="ALVHRQESWTYRELYRWVGATAVHLREKGVQPGELVGLLLERNE
                     HLLPSLLGILAAGAAYLPIDPGYPAERIRYMLEDSGIMRVVTEPSLTHVVQQVGDTGA
                     IDVTTIDRTVPESAGMCSSAAATDLAYMIYTSGSTGNPKGVLIGQENVVNFVRGVTDR
                     IPFIPGDVLLCVTTVSFDIFVLETIVPLLNGLTVVLAAKDEQRDPLTLCRMIVEKGVS
                     CLQMTPSHLQLLLAASAGKGLPGSIRLLMVGGEAFPSGLLKTLQENHRCGIYNMYGPT
                     ETTVWSVIGDLTHADAVRLGKPMANTYIRITDSFQRLVPRGTAGELCIGGAGVSRGYW
                     RRDALSEEKFIPDPAGTGERFYRTGDLARWEPDGVLCFLGRLDSQEKIRGYRIEPGEI
                     EHQLKNMPGILETAVVARESNGIKQLVAYYTASQPVEQAGMRQYIADRLPEYMVPAYF
                     IPLSAMPLTPNGKLNRKALPAPVMTAGEDYAPPSGHTEVSLVDMWAEVLKIDKTVIGV
                     HKNFFELGGTSLHAMALMLEIRKAFDVQISLAKFFAKPTVHALGRSIQVTNLSMNVKS
                     TRDKVTI"
BASE COUNT          828 a         1254 c         1025 g          827 t
ORIGIN      
        1 tagcggtcca gaccgctgaa ggagatgccg gagggatgga tcaccacctg tatcccgtta
       61 cacagcgtcc agaaaagctc cagcaccgat atatcgaagg acgtgctcgt caccgccagc
      121 aggcagtcgt cttcgcccgc cggcagccgc tcattcagtc ccgcaaaaaa attcaccaca
      181 ttccggtgag cgatcattac cccctttgga ttacccgtcg atcccgatgt atagatcagg
      241 tagcacagat ctccggaacc attcacccgc ggcagatcat ccgtctcctc ttccccacgc
      301 ggactcacga tgcttccgcc atatcccgac ggcccctctc ttcccctgtc cacgatcagc
      361 acctccgccc cgctgtccga gagtatatag gacgtccgtt ccgccggata acccggatcc
      421 atcggcaggt atgctcctcc cgccttcagg atacccagca atcctaccat cagttccagc
      481 gaccggtcca tcatcacccc aaccaccttg tccgggccta ctccatattc gcccctcagc
      541 cgctgcgcca acccgttgct ccggcggttc agttccccat aggtcagcga ctcaccttca
      601 tacacaaccg ctatttcttc cggacgctgc gccgcctgcg tctcgaacag ggtcaccaac
      661 gtctcctccc gtccatactc ccggctcgta tcgttgaaac ccgacaacaa ctcctcctgt
      721 tcaacctccg gcagcagctc gatcgcccca agcttcacct ccgggtcctc cgtcaccgcg
      781 gcgactatcc gctggaagta tcccgcaaat cgctccaccg tatcccgacg gaacaggttt
      841 gtcgaatatt cgaaatgcag cgtcacgtcg ctttccgcct ccagcacata aaggctcaga
      901 tcgagcttgg ccaccgcgtg gtcaaggcga tagggtcggc tgatcacccc gggaaacctc
      961 gcggagccag ggctgacatg ggtaaggtcg aacaatacgt cgaacagggg attccggctc
     1021 gcatcacgct ctatcttctg ctggtcgata agctcctcgt aaggataatc ctggtgatcc
     1081 agacaggcca gcaccctgct gcgcacctgc gccagaaatt gaaaaaagga cagttcgccg
     1141 gcgggaaggt tacggatagg cagcgtattg gcgaacatcc cgacgacgtt ctccagacct
     1201 gcgtaatgtc ttcccgcggt ggggatgccc acgacgatat cttcctgccc gctcatgcgt
     1261 gaaagaaata tataatagac cgccaaatag atcataaagg aactggcgcc attctgcccg
     1321 gccagctgct tcatccgccg ggccatctcc ccatccaggg acaggtccag ggaatcgccc
     1381 tcgtagccga cgaccggggg ccgcgtcata tccgcgggca gttccagcgg aacggtctgt
     1441 ttgcggaaca tttccaccca gaaatctttt tgttggtcgg tggccatttg ctgatcgggt
     1501 ccctgacgcc aggcggcata atccttgaac tgcagctcca gcgcaggtaa aagcgcgtcg
     1561 gtgtaaagga gcatcaggtc cctggtgagg atatcctggg atgccccgtc cgcaatgata
     1621 tgatgcgtgt ccatcaccag cagatagcgg tcctgtcccg tcccgatcag ggcgacgcgc
     1681 atcagcggcg cgctctccag gctgaaaggg cggatatagg tccggagcag gcccgcggcc
     1741 tcttccggac ggcaatagaa acgatcgatt gcgaagggga cctcgccgga tatacgttga
     1801 actggcattt catccaccgt atcgaaccag gtccgcaggc tctcgtgacg cgcgatcagc
     1861 cggcggaaag cgcgctccag ccgcggtacg tccattctgc cttctatctc cagtatcttc
     1921 ggcaggttat aagcgatgga ggacctgtcg aactcataaa ggaaatagag ccttttctgg
     1981 gcggccgaca gcgcatagtg ttcagcggta gctacccggc ttatcggttt gaaaggagcc
     2041 gccgccccgg tatctttcaa caaagcaagc agctggcgct tattctccct gattacggat
     2101 aacaattcct gcgtgagtcc ctggcgcgga tcgtagatgt ccaattcgga ccctcgtgct
     2161 tccagcgaga tatccaactc cgccagctta ttgagcagct cttccatatc tttagattgt
     2221 gaccttgtcc ctggtcgatt ttacattcat tgacagattg gtcacctgaa tgctgcgacc
     2281 cagtgcatgt acggtcggtt tggcaaagaa cttcgccagg gatatctgta catcgaacgc
     2341 tttccggatc tccagcatca gcgccatcgc atgtagcgag gttccgccga gctcgaagaa
     2401 attcttatgc acgccgatga ccgtcttatc tattttgagc acttccgccc acatgtctac
     2461 caggctgact tcggtgtgcc cggatggagg agcatagtcc tcgccggccg tcatcaccgg
     2521 ggcgggaagg gcctttctgt ttaacttgcc attgggtgtc aggggcatgg cggacagcgg
     2581 aataaaatag gccggcacca tatattccgg cagcctgtcg gcgatatact gtcgcatgcc
     2641 tgcctgctcc accggctgag atgcggtata gtacgctacc aactgtttga tcccgttgct
     2701 ttcccttgcc accacggccg tctcgaggat accgggcata tttttgagct ggtgttcgat
     2761 ctcaccgggc tctattcgat agcccctgat cttttcctgg ctgtcgagcc tgcccaggaa
     2821 acacaggacc ccatccggtt cccatctcgc gagatcaccg gtacggtaga aacgctctcc
     2881 cgtacccgcc ggatcaggaa tgaacttctc ctcactgagc gcgtcgcgcc gccaatatcc
     2941 ccgtgatacg ccggccccgc cgatacaaag ctcgcccgcg gtaccccgtg ggaccaaacg
     3001 ctgaaagctg tccgttatcc ggatataggt attggccatc ggcttgccca ggcgaacagc
     3061 atcggcatgg gtaaggtccc cgatgacgga ccagacggtc gtttctgtag gtccatacat
     3121 attgtagatg ccgcagcggt ggttctcttg cagggttttc aaaagacccg acgggaatgc
     3181 ttcgcctcct accatcagta accggatact ccccggcaga ccctttccgg cgctagcggc
     3241 cagcaacaac tgcagatgtg aaggcgtcat ttgcagacag gaaactccct tctccacgat
     3301 catgcggcat agggtcagcg gatcgcgctg ctcgtccttc gccgccaata cgacggtgag
     3361 gccattgagc agcggcacta tcgtctccag gacaaagatg tcgaaggaca ccgtggtgac
     3421 gcaaagcagg acatcgcccg ggataaaggg tatcctgtcg gtaacgcccc tgacaaaatt
     3481 gacgacattc tcctgtccga tcaggacgcc tttcggattg cctgtcgagc cgctagtata
     3541 gatcatatag gcgaggtccg tagccgcagc cgacgaacac atgcccgccg attccggaac
     3601 cgtccgatcg atggtcgtta catctatcgc accggtatcg cccacctgct gaacgacatg
     3661 ggtcagggaa ggctccgtca ccaccctcat gatcccgctg tcttccagca tgtacctgat
     3721 cctttctgca ggatagccgg ggtctatggg cagataagcc gctcccgcag caaggatgcc
     3781 gagaagagaa ggtaaaagat gttcattcct ttccagcagg agcccgacca actcacccgg
     3841 ctgtacgccc ttttcccgca gatggacagc cgtagcgcca acccatcggt agagttcccg
     3901 gtacgtccag ctctcctggc ggtggaccag ggcg
//
LOCUS       DASYSV010000212         3121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_421108, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000212 DASYSV010000000
VERSION     DASYSV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421108"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VGQ51_06490"
     CDS             <1..739
                     /locus_tag="VGQ51_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036244.1"
                     /translation="RQRIAWLIAICLIVCTLPSVGQERENPRKIQTFTAAQMRANFAK
                     FTQDAAFGALYKDARSKGFQQIIREDAMWGYEGVVTDAQGKEQKIFFCAYDFYNPKAS
                     NGQGCSMIWKKVGDRLYRAYLIFPEGEKDFDKAMSGAMEWFAEDDGKVQRAHSWGTCF
                     LKCVHTNGTAPGLSVDIKNGKLLVGTSTFKINCATACFTGAIVCSGVAAVVAASTVGV
                     PFAIAELAVCTGVTCAPCLAACAIGCM"
     gene            complement(751..1959)
                     /locus_tag="VGQ51_06495"
     CDS             complement(751..1959)
                     /locus_tag="VGQ51_06495"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV9036245.1"
                     /translation="MPLRKLCLTACLFCCLTATALAQDSLLSKSDIIKLDFNTLAEKK
                     NRLREKDPALIPAYRQLLQDADKLLDQAPLSVMQKTAIPPSGDKHDYMSIAPYFWPDP
                     SKSDGLPYINKDGVVNSEVRKYTDKVNLPVVCENIYLLSLAYYYSNNEKYAKRASRLT
                     EVWFLDPATRMNPNLNFGQAVKGVTDGRAAGLIDSRFFIWAIDGIRLLQTSPHWTARD
                     QTTLRQWFSSFLTWMQTSTIGQKEKAARNNHGIWYDAQTLAMALFVDSMDLANKIVLR
                     DLDRLDQQMDTSGLFPLELGRTTSLHYSVFILNAFTIVAQLSEQTHTNLWTTTTPSGK
                     SLKKAFQAIIPYITREKDWPWPQIHEFNYLDAVPLLVAGRSGFQCLSCGDALKRIEGE
                     TYHRSLFNLL"
     gene            complement(1971..2318)
                     /locus_tag="VGQ51_06500"
     CDS             complement(1971..2318)
                     /locus_tag="VGQ51_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV9036246.1"
                     /translation="MKTNAPPGNFIENDNLPWEEIEKGIYRKVMAYDEKLMLVKVKFE
                     TGKIGTLHQHHHSQISQVESGVFEIEIDGLKKTLKQGDAYYVPPDTMHGAKCIESGVL
                     IDVFSPMREDFIQ"
     gene            complement(2330..>3121)
                     /locus_tag="VGQ51_06505"
     CDS             complement(2330..>3121)
                     /locus_tag="VGQ51_06505"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9036247.1"
                     /translation="TRATTSAKDPNDYTNALAECNKVIGSGQYILLPNYKSIFDWTNK
                     FPAKPENIFSVQFAEPPSVGNIIIRMLTSSKTTPAGSASFYVPAAFYNSYIPADSIRR
                     NFSITNKDKSANGTITTDPLYFYSKFSTDPTWTAQSNNSMANWIITRYADDLLMQSEA
                     LHFLNPLDSNQFNGINAVRARAGLTGAYQLNFSNTPGPDDFITALLNERAWELCMEGH
                     RRYDLIRLGRIQSALLAGKGVTVPSPNPLLPIPFAQVALDSLLGK"
BASE COUNT          688 a          742 c          888 g          803 t
ORIGIN      
        1 gagacaaaga atcgcttggc tgatcgccat ttgcctgatc gtctgtacgc ttccttcggt
       61 tggccaggag cgcgaaaacc caaggaagat ccagacattc actgctgccc aaatgagggc
      121 caattttgca aagttcacgc aggatgcagc ctttggcgcc ctgtataaag acgcccgaag
      181 caaaggtttt caacagataa ttcgcgagga cgctatgtgg gggtatgaag gcgtcgtgac
      241 cgatgcgcag gggaaggaac agaagatttt tttctgtgcc tacgatttct acaatcctaa
      301 ggcctcgaat ggccagggct gttcgatgat ctggaagaaa gtaggcgacc gcctctaccg
      361 tgcgtacctt atctttcccg aaggggagaa agacttcgac aaggccatga gcggcgccat
      421 ggaatggttt gccgaagacg acggcaaggt tcagcgtgct cacagctggg gcacctgttt
      481 tctcaagtgc gtacatacga atgggacggc gccaggcctt agcgtcgata ttaagaatgg
      541 caagctgctg gtgggcacca gcactttcaa gatcaattgt gcaacggcgt gttttaccgg
      601 cgctatcgtt tgttccgggg tcgcagcagt ggttgccgcc tcaaccgtcg gcgtcccttt
      661 tgccattgct gagctggccg tatgcacggg agtgacctgc gctccctgtc tggccgcctg
      721 cgccattgga tgtatgtaag agccggcggg ttataataga ttgaacaagg atcgatggta
      781 tgtttcgcct tcgatccttt ttaatgcatc cccgcaggaa aggcattgga atccggacct
      841 gcccgctacc aggagcggca cggcgtccag gtagttgaat tcatggattt gcggccaggg
      901 ccagtctttt tccctggtta tgtatgggat gatagcctgg aatgcttttt tgagcgattt
      961 gccggatggg gttgttgtcg tccataagtt ggtgtgggtt tgttcggaga gttgcgcgac
     1021 aatagtaaat gcattgagga tgaaaacgga atagtgaagc gaagtggtac gacccagttc
     1081 aagggggaat aagccactcg tatccatttg ttgatccagc cggtcgagat cgcgcaggac
     1141 gatcttgttt gccagatcca tgctatccac gaacagggcc attgcaaggg tttgcgcatc
     1201 gtaccatata ccatggttgt tgcgggcagc cttttctttt tgcccgatgg tacttgtctg
     1261 catccaggtg aggaaggatg agaaccattg cctgagggtg gtttgatccc gtgccgtcca
     1321 gtgaggggaa gtctgaagca gcctgatccc atcgatcgcc cagataaaga accgcgagtc
     1381 gatcaatcca gcagccctgc cgtccgtgac cccttttacg gcctgcccaa aattaagatt
     1441 agggttcatg cgggtggccg ggtccaggaa ccaaacctcc gtcaaccggc tcgcccgctt
     1501 tgcatatttc tcattgttgg agtaatagta agccagggat agcaggtaga tgttctcaca
     1561 gacgaccggc aggttgacct tgtcggtata cttccgcact tccgagttga ccacgccgtc
     1621 tttattgata taagggagtc cgtcggattt cgacgggtcc ggccagaaat aaggagctat
     1681 gctcatgtag tcatgcttgt cgccgctggg cgggattgcg gtcttttgca tgacggaaag
     1741 gggtgcctgg tccaggagtt tgtcggcgtc ctgcagcagt tgtcgatagg ccgggatcag
     1801 ggcaggatct ttctccctca gccggttctt tttttcggcc agcgtgttga aatcgagttt
     1861 tatgatatcc gacttcgaaa gcaacgagtc ctgcgccagg gcggtggcgg tcagacagca
     1921 aaaaagacag gcggtaaggc aaagttttct gagcggcata ggaattcaat ttactggata
     1981 aagtcttccc gcatcgggct gaatacatcg atgaggacgc cggattcgat acatttcgcc
     2041 ccatgcatcg tatcgggagg gacgtagtac gcgtctcctt gtttcaacgt tttcttcagg
     2101 ccatcgatct caatttcgaa caccccgctt tccacctgag aaatttgtga atgatggtgc
     2161 tggtgcaacg ttccgatctt tccggtctcg aattttactt tgacgagcat cagcttttca
     2221 tcataggcca tgactttccg atagataccc ttttcgatct cttcccatgg caggttgtca
     2281 ttttcgataa agttaccggg cggcgcattc gtcttcatga attattgttt tattttccaa
     2341 gcagagaatc aagggcgacc tgggcgaacg gaatgggtaa cagcgggttt gggctgggta
     2401 ccgtgactcc tttgccggct aatagggcgg attgtatccg gcccagccgg ataaggtcat
     2461 atctgcgatg gccttccata caaagttccc aggcgcgttc attcaacaag gcggtgatga
     2521 aatcatccgg ccccggagta ttggaaaagt tcaattgata ggcgcctgtg agacctgctc
     2581 ttgcccgtac ggcatttatt ccgttgaatt ggttcgaatc cagcggattg aggaaatgca
     2641 gggcttccga ctgcatgagc aggtcgtcgg cataccgggt aatgatccaa ttggccatcg
     2701 agttattgct ttgcgccgtc caggtcggat cggtgctgaa ttttgaataa aagtacaacg
     2761 gatccgtggt aatcgtgcca ttggcgcttt tatccttatt ggtgatgctg aaattcctcc
     2821 ggatggaatc cgccgggata taactgttgt aaaatgccgc aggcacatag aaggaggcgg
     2881 accctgccgg cgtcgttttg gaggaggtca gcatccggat gatgatattc cctacactgg
     2941 gcggctctgc gaattgaacc gagaagatat tctccggctt ggcaggaaac ttattggtcc
     3001 agtcgaagat cgacttataa ttcggcaata atatgtattg accggaaccg atgaccttat
     3061 tgcattcggc cagtgcgttg gtatagtcat tcgggtcttt tgcggaagtg gtagccctgg
     3121 t
//
LOCUS       DASYSV010000213        10005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_39687, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000213 DASYSV010000000
VERSION     DASYSV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39687"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VGQ51_06510"
     CDS             <1..651
                     /locus_tag="VGQ51_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036248.1"
                     /translation="RSFAQTAVELIPSVGYTFADRNDYYSAYSRIADGVSYGGSIKFN
                     VNRGFGIEVLYSHMNTTSGLYNYGPDHTPISATNLSFDYIMAGPVQSFTIPNSTVRPF
                     IGAMLGAAIMTPAMNSGYTQQTNFAMGFQLGTNIYVSPRVGIQLKAQLLSPVDAAGTS
                     FFFSNYGSGVGINGYSSTYQFSLGGGLIIGLGRVLPAEAFHPARRPHPRYYPRYYY"
     gene            792..1934
                     /locus_tag="VGQ51_06515"
     CDS             792..1934
                     /locus_tag="VGQ51_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV9036249.1"
                     /translation="MEFAFDELTKQVGQTARQFAAQHIKPFVMEWDEAQTFPHQLFKQ
                     LGQLGMMGVVVPEEYGGSGLSYIEYVTIISEISSVCGAIGLSLAAHNSLCTGHILAFG
                     NKQQKEKYLHRLASGEALGAWGLTEANTGSDAGNMQCTAVKDGDHWVINGTKNWITHG
                     ISGDIAVVVARTGEPRKKNNATAFVVERGAKGFSGGKKENKLGMRASETAEMIFDNCR
                     IPDSQRLGEVGDGFHQALAVLDGGRISIAALSLGIAKGALAAALQYSKERHQFDQPIS
                     NFQGISFKLADMATEIAAAELLTMQAADLKNRHLPMTREAAMAKYYASEVAVRAATEA
                     VQIFGGYGYTKDFPVEKFYRDSKLCTIGEGTSEIQKIVIAREVLKG"
     gene            complement(1997..2170)
                     /locus_tag="VGQ51_06520"
     CDS             complement(1997..2170)
                     /locus_tag="VGQ51_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036250.1"
                     /translation="MEDLIEKLKAQGLTEEQAYKAIEVIKNFTKEKFPLFAGAIDKLF
                     DKYGPKTEDDFMP"
     gene            complement(2174..3409)
                     /gene="clpX"
                     /locus_tag="VGQ51_06525"
     CDS             complement(2174..3409)
                     /gene="clpX"
                     /locus_tag="VGQ51_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV9036251.1"
                     /translation="MAKNTLHCSFCGRNRDEVKILIAGQEGHICENCVEHAQEIIQQE
                     LMIREDSTGSSSFKLTVKKPIEIKRILDEYVIGQEDAKKVLSVAVYNHYKRLQQKVVE
                     NEVEIEKSNIIMVGETGTGKTLLAKTIARLLNVPFAIVDATVFTEAGYVGEDVESILT
                     RLLQVCNYDVAAAERGIVYIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGTDVL
                     VPPQGGRKHPEQKLIKINTQNILFICGGAFDGVDKIIARRINTNAIGFNVNKEQQEAA
                     KKNLLQYVNAPDLKSFGLIPELLGRLPVVTHLDPLDKPTLRSILTEPKNSLIRQYKKL
                     FELEGIQLSFELDVLDFMVDKAVEYKLGARGLRSICESILTDAMFELPSAKEDKFHLD
                     LEYAQKKFDKSKMNLLKVA"
     gene            complement(3510..4148)
                     /locus_tag="VGQ51_06530"
     CDS             complement(3510..4148)
                     /locus_tag="VGQ51_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEV9036252.1"
                     /translation="MNYSTYWGSRLRMDDEPDEKEPKDEKGPEFGMLAKKLEKYFFES
                     RSVELWGPVDDKSAKDVVTKLRLLDADKPGEEIKFFINSPGGVVTSGFIIYDTMKLLK
                     SPVSTICMGLAASMGSILLSGGEKGRRFVYPLSEIMIHQPSLGGYMQGVSIDLEITAR
                     QTRRVKDISARILAENCGKRVEQVMKDFDRDYWMDAKEAIEYGIVDHIFEKV"
     gene            complement(4184..4876)
                     /locus_tag="VGQ51_06535"
     CDS             complement(4184..4876)
                     /locus_tag="VGQ51_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEV9036253.1"
                     /translation="MNPSNEFEKYAVKHRGLSSNTLNDYNKYVVTNLTPNIIEERPMN
                     VAVMDVYSRLMMDRIIFLGYPINEEVANIVTAQLLFLDSTDRHRDIQMYINCPGGSVY
                     SGLGVYDTMQYVTPDVATICIGMAASMGQVLMCAGTKGKRTALKHSRVMMHQPSGSIG
                     GQTTDIEITVAEIKKLRRELYDITAFHTGKTAKQVEKDSDRDYWLTAQEAKEYGLVDE
                     VLLLNPKKEARS"
     gene            complement(5025..6413)
                     /gene="tig"
                     /locus_tag="VGQ51_06540"
     CDS             complement(5025..6413)
                     /gene="tig"
                     /locus_tag="VGQ51_06540"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV9036254.1"
                     /translation="MATVTRENIGLLTDKITVSISKGDYLPSFERTLKQYGKQANIPG
                     FRKGMVPAGLIKKMYGNSVFTDEVLKTVEKELTSYMSNEKLEIFAQPLPLPENDARQI
                     DINNPADYSFAFEVGLKPAFALADLGSAKPTKLKIIVTDAMIDEEVERMRTRNGKMTE
                     PESVTGDENVLNLHFSESDASGQPVEGGISKDNSLLVKYFNEATRPKWMGLKKGDFLV
                     LQPSAAFEEKEREWVMHDLGLDHAGTDAGNGADKYFRTEITKVGFIEKPEVDEAFFQT
                     VYPGREIASENAFRDAVKDDIAAYWEGQSRNQLQHGLYHILLDQTKIEFPEDFLKRWM
                     QSGGDKPKTQSEVEREFPSFVNQLKWTLIIDKIIQDNGIEVGPDEIRAFAKQQLFGYM
                     GMPAGAEEQPWVADYVQRMMQDRKFVEDTVHRIRTEKVFGWAETQVHPTEKPVSKEDF
                     EHEVQEHEHHHH"
     gene            complement(6487..6975)
                     /locus_tag="VGQ51_06545"
     CDS             complement(6487..6975)
                     /locus_tag="VGQ51_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036255.1"
                     /translation="MQVKFALLALLSVAGPLILSAQTSDAEAEAVINLLGIQKREAIA
                     RLVPVYGKDSAAFWKIYEEYQQGNRATAKDRLQLYEGTALSYQRMTPQVADSLGQLYF
                     KNRMDQERTLETYYKKIKTATNAVVAFEFYQAEIYLLTMIRAQIMQKIPTYGQLQPAP
                     GR"
     gene            complement(7079..7966)
                     /locus_tag="VGQ51_06550"
     CDS             complement(7079..7966)
                     /locus_tag="VGQ51_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036256.1"
                     /translation="MPPKYSISTDNTANKSQRQSGKFSKAAKTTGTLSKPKDFRGKKL
                     HEAGLPVDQYALLRNAIPNADGTSTLQVTNKNPSLLSYHGSKVNTAVAEKMISSADGA
                     RTSTSILLSGANDTAVWHSGYIEKKTTGQPQAHNLVRKANFKMASTSTSTKGVSDRSV
                     SVAIGSIALASVAPAELADKAPRSSSTKEVAWANSLEERRNEVKLRIDGITLAPAERS
                     FVNKNVSEYFSSISPSGKRRPQNKRSYSPPRKTKGGTYFKGGGYTDPDPKKPKLSPPS
                     KDAELTAYITQPLRKQNKS"
     gene            complement(7970..9616)
                     /locus_tag="VGQ51_06555"
     CDS             complement(7970..9616)
                     /locus_tag="VGQ51_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036257.1"
                     /translation="MFKKKDPYSFSLFRDLVLKPNVIGNFFVEYQVTKYDDIQIKDFQ
                     SLNIKNTSQKYLIHCTQKINIFNQQGLDVSPIPYSDTPSFENYPVLVNTFIYPEKPDG
                     AEGLDIQLLQYSPQTVNTKIQQSGSSADTDGKTNGTSVSNTVGSSTSETNSYGVSISV
                     GLTEQSDSMSANYEHSTTETHDQSSTQGRDYSRSQSREDSSSSMMSIKDWGSYALVNP
                     TNKAVVWTFGQEYPWDAIQCRTEKAKDSDKNNQKEIIVSPAMAARLYDKTEKVLYPPS
                     ELSIFGINFVMKGLWLITIDNYADDEVTFKHVVNYFSGSHFLDSNGLSVYIDKQAVSL
                     RFPQDESLETKIDTALLALDPVDVSGRNPVIGFIPGKFLRKPFPLNRDGAKGAFKIIS
                     TANDLLIRDTTTYPEFGYEGLGFTAVETGLLANFSAEPLPLSMTLYFKITDNWNEYTL
                     FMKHWKTGDASSTGVKLSIVINGDTDATIVKYVDALEAEGGENNLSSINLRNLDYATV
                     DYHDYLQLGVNTIEITLRPIDPAGAARCGYQIRGISIEKT"
     gene            complement(9621..>10005)
                     /locus_tag="VGQ51_06560"
     CDS             complement(9621..>10005)
                     /locus_tag="VGQ51_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036258.1"
                     /translation="LCGRTLIGAAAPPYPAPRQSDGSFPNFAPDADWEIGDTGGEFQH
                     QLVTTEIPSHNHFFDSSQWYDFGLGPSSWQPDDDGRNVMCQQATAGDPNKVIAPTGGD
                     GAHNTMQPYYAVNYIIYGGEPDNTK"
BASE COUNT         2134 a         2703 c         2508 g         2660 t
ORIGIN      
        1 cgttccttcg cgcagactgc cgtcgagctg atcccttctg tcgggtatac ctttgcagac
       61 cgcaacgatt attacagcgc ctatagccgc atcgcagacg gcgtaagcta tggcggatcg
      121 atcaaattca acgtcaaccg cggttttggt atcgaagtgc tctacagtca tatgaacacg
      181 acctcggggc tttataatta cggccccgac catactccga tctccgcgac caacctgagc
      241 ttcgactaca tcatggccgg tcccgtccag tccttcacga tccccaactc cacggtgcgt
      301 ccctttatcg gcgccatgct gggggctgcg atcatgacgc ccgccatgaa ttcaggctat
      361 acccagcaaa cgaattttgc catggggttc cagctcggca cgaatatcta cgtttctccc
      421 cgggtgggta ttcagctgaa agcccagctg ctgtcccctg tcgacgcagc cggcacaagc
      481 ttcttcttca gcaattatgg cagcggagta ggtatcaacg gctattcgtc gacttatcaa
      541 ttcagcctcg gcggcggcct gatcatcggg ctcggcaggg tccttcccgc agaggcgttt
      601 catccggccc gtcgcccgca cccccgttat tacccgcgat attattatta gcatagatta
      661 gttggacgga tcagtacccc ttaggccgcg cgcgagcgcg gttttttcat atgcccgcaa
      721 ctgcggggcc tcgccgcgca atactaacac ccgttagtcc gtttgaagaa attcacctat
      781 attgtgcccc catggaattt gcattcgatg aacttacaaa acaggtaggc cagacggccc
      841 gccaattcgc agctcagcac atcaaaccgt tcgtcatgga atgggatgag gcccaaacct
      901 tcccgcatca gcttttcaaa caactgggac agttgggcat gatgggcgtc gtcgttcccg
      961 aagaatatgg cggctcgggc ctgtcctata tcgaatacgt cacgatcatc agtgagatct
     1021 cgtccgtctg tggcgccatc ggacttagcc tggccgcgca caattcactc tgcacggggc
     1081 atatcctcgc ctttggtaac aaacagcaaa aagagaaata tctccatcgt cttgcatccg
     1141 gtgaggcgct gggcgcctgg gggttgacag aagctaacac gggcagcgat gccggcaaca
     1201 tgcagtgcac agccgtaaag gacggcgacc actgggtgat caacggtacg aagaactgga
     1261 tcacgcatgg catcagcggc gacatcgccg tggtggtggc ccgcaccggc gagccccgta
     1321 agaagaacaa tgccaccgct tttgtcgtag agcgaggcgc caaaggtttc agcggcggca
     1381 aaaaggagaa caagctcgga atgcgagcca gcgagacagc cgaaatgatc ttcgataatt
     1441 gccgtattcc cgatagccaa cgcctcggtg aggtaggcga tggtttccac caggccctcg
     1501 ccgtactcga tggaggacgc atctccattg cagcgctttc gctgggtatc gctaaaggcg
     1561 cactggcggc cgcattgcaa tattccaagg agcgccacca gttcgatcag cccatcagca
     1621 atttccaggg tatttccttc aagctggccg atatggccac cgaaatagcc gccgccgaac
     1681 tgctgaccat gcaggccgcc gatctcaaga accggcattt gccgatgacc cgcgaggccg
     1741 ctatggcgaa atattatgcc agtgaagtag cggtgcgcgc cgccacggag gccgtccaga
     1801 tcttcggcgg ctatggctat acaaaagatt tcccggtgga aaaattctat cgcgacagca
     1861 aactatgcac gatcggtgaa ggcacctccg agatccaaaa gatcgttatt gcacgggaag
     1921 tgctgaaggg gtaaggggtt cccacgcatc gggcgacgac ccccggagaa aggcgcgagg
     1981 gcctgtgcgg ccggcctcac ggcataaaat catcctccgt cttcggccca tatttatcga
     2041 atagtttatc gatagcgccc gcgaacagcg gaaatttttc tttcgtgaaa ttcttgatga
     2101 cttcgatggc cttgtaggcc tgctcttccg tcagaccctg agccttcagc ttttcgatca
     2161 gatcttccat tcgttatgct actttaagaa gattcatttt gctcttgtcg aactttttct
     2221 gcgcatactc cagatcgaga tggaacttgt cttccttggc cgaaggcagt tcgaacatcg
     2281 catccgtcag gatactctcg caaatgctcc gcaaaccacg cgctccaagt ttgtattcca
     2341 cggctttgtc gaccatgaaa tccaatacat cgagctcgaa gctgagctga ataccctcga
     2401 gctcgaacaa ctttttgtac tggcggatca gcgagttctt cggctccgtc aggatggacc
     2461 gcagggtcgg cttatccagc ggatccagat gcgtgaccac cggcaagcgt cctaaaagct
     2521 ccggtatcag accaaatgac ttcagatcgg gcgcattcac atactgcaac aggttcttct
     2581 tggcggcctc ctgttgctcc ttattcacgt tgaagccgat ggcattcgta ttgatgcggc
     2641 gggcaatgat cttgtccaca ccatcaaaag cgccgccgca aataaagagg atattctgcg
     2701 tattgatctt gatcagtttt tgctcggggt gtttcctgcc gccttgcggg gggaccagca
     2761 catcggtgcc ttccagtaat ttcaataacc cttgttgaac gccttcaccg ctgacatcgc
     2821 gggtgataga agggttgtct cccttccggg cgatcttgtc gatctcgtcg atataaacga
     2881 tgccccgctc agccgcggcc acgtcataat tgcacacctg caacaggcgg gtcagtatgc
     2941 tttccacatc ttcccctaca tagcctgcct ccgtaaaaac ggtcgcatcc actatcgcga
     3001 atggaacgtt caacagccgg gcgatcgttt tggccaaaag ggttttaccc gtccccgttt
     3061 cgcccaccat gatgatattg ctcttctcga tctcgacctc attctccacg actttctgct
     3121 gcagacgttt gtagtggtta tacacggcta ccgacagcac tttcttcgcg tcttcctgtc
     3181 cgatgacata ttcgtccagt atacgcttga tctctatcgg cttcttaacg gttaatttga
     3241 atgagctgct acccgtcgaa tcctcccgga tcatcagctc ctgctgtatg atctcctgcg
     3301 catgctctac gcaattctca caaatatgcc cctcctgacc cgcaatgagt atcttcactt
     3361 catcccggtt gcgcccacag aaggaacagt gcaaggtatt tttcgccata ttacaaagtt
     3421 acccaactta ttcaattata ccagtagggt ggaaaaaaga gtaggggcga ccgccaggcc
     3481 gcccgctatt gaaaatcaat tagttgcgat cagaccttct caaaaatatg atccacgatg
     3541 ccatattcga tcgcctcttt ggcgtccatc cagtaatcgc ggtcgaagtc cttcatcacc
     3601 tgctcgaccc ttttaccgca attctcagcc agtatccggg cactgatatc cttgacccgc
     3661 cgggtctgtc tggccgtgat ctccagatcg atgcttacgc cttgcatata tccgcccagg
     3721 ctcggttgat ggatcatgat ctcggacagt ggataaacga aacggcggcc cttctctccc
     3781 ccgctgagca ggatcgagcc catggaagcc gcaagaccca tacagatcgt gctcacgggg
     3841 cttttcagca gcttcatcgt atcgtaaatg ataaagcccg aagtgacgac accgccggga
     3901 ctgttgataa agaatttgat ctcctcacct ggtttatccg catccagcag ccgcaatttg
     3961 gtgacaacat ccttggccga tttgtcatct accggtcccc atagttctac cgatctgctt
     4021 tcgaaaaaat acttctccag cttcttcgcc aacattccga attccggtcc cttctcatcc
     4081 tttggctctt tttcatcggg ctcgtcatcc atccgcagac gactgcccca atatgtcgaa
     4141 taattcattg tcctgtcttt tttttgtttg ataagtatat ggtttacgat ctggcttcct
     4201 tcttgggatt cagaagcagc acttcgtcga cgagaccgta ttccttcgct tcctgggcgg
     4261 tcagccagta atcgcgatcg ctgtcctttt ccacctgttt ggccgtttta ccggtgtgga
     4321 aggcggtgat atcatacagc tcgcgtctca gttttttgat ctcggcgacg gtgatctcga
     4381 tatcggtggt ttgtcccccg atggacccgc tgggctgatg catcatgacc cggctatgct
     4441 tcagggcggt ccgtttaccc ttggtgccgg cgcacatcag gacctggccc attgaagccg
     4501 ccattccgat acaaatggtg gctacgtcgg gagtgacata ttgcatggta tcgtaaacgc
     4561 caagtccgga atagacgctg ccgccgggac agttgatata catctggata tcccggtggc
     4621 ggtccgtgct gtcgaggaac aacagctggg ctgtcacgat attggctacc tcttcgttga
     4681 tcgggtagcc caaaaagatg atacgatcca tcatgagacg gctataaacg tccatcacgg
     4741 ccacattcat agggcgttct tcaataatgt tcggtgtaag attggtaaca acatacttgt
     4801 tgtagtcatt caatgtgttg ctggacaggc cgcgatgctt cacggcatat ttctcgaatt
     4861 cgtttgatgg attcattgtt gatctattgg ttcaataatg gtaaagttcc attcaaatct
     4921 ccgtccaaac tttcaaaccg ccaaaaatct gccattctgt ccattttggc cgggcttgcc
     4981 gtgcaaatca aaaacccggc cgaaggccgg gttccattca ttatctagtg gtgatggtgt
     5041 tcatgttcct gcacctcatg ctcgaagtct tccttgctga cgggtttttc ggtcggatgc
     5101 acctgcgttt ccgcccatcc gaagaccttt tcggtccgga tccggtgtac cgtatcttcc
     5161 acgaatttcc ggtcctgcat catgcgctgc acataatcgg ccacccaggg ttgctcttcg
     5221 gcgcctgcag gcatacccat atagccgaat agctgctgtt tcgcaaaggc cctgatctcg
     5281 tcgggtccga cttcgatgcc gttatcctgg atgatcttgt cgatgatgag cgtccatttc
     5341 agctggttga cgaaggaggg aaactcccgc tcgacttcag actgggtctt gggtttgtcg
     5401 ccgccgctct gcatccagcg cttcaggaag tcttcgggaa attcgatctt cgtctgatcc
     5461 agcaggatat gatataaacc gtgctgcaat tgattccggc tctgaccttc ccagtacgcg
     5521 gcgatatcgt cttttacggc atcgcggaag gcgttttcgg aagcgatctc cctacccgga
     5581 tagacggttt ggaaaaaggc ctcatccact tcaggctttt cgataaagcc cactttagtg
     5641 atctccgtcc ggaaatattt gtcggcccca ttacccgcgt cggtcccggc atgatcgaga
     5701 cccagatcgt gcatgaccca ttccctttcc ttttcttcaa atgccgccga aggctgcagc
     5761 acgagaaaat cccccttttt cagtcccatc catttcggac gggtggcctc attaaaatat
     5821 tttaccagca gtgaattgtc ttttgaaata ccgccttcca ccggttggcc cgaggcatcc
     5881 gactcagaaa aatgtaaatt cagcacgttc tcgtcgccgg tgacgctttc cggttcggtc
     5941 attttgccat tccgcgttct catccgctcg acctcttcat cgatcatggc atcggtgact
     6001 atgatcttca gtttggtcgg cttcgccgat cccaggtccg ccaatgcaaa ggccggcttc
     6061 agccccacct cgaaggcaaa ggaatagtcg gcgggattat tgatatcgat ctgccgggca
     6121 tcgttctcag gcaatggcag cggttgcgca aagatctcca gtttctcgtt ggacatatag
     6181 gaggtcagct ccttttcgac ggtcttcagc acttcatcgg tgaataccga gttgccgtac
     6241 attttcttga tgagccctgc gggcaccatc cccttccgga aacccggaat atttgcctgt
     6301 ttgccatatt gtttcagcgt ccgctcgaaa gacggcagat aatcgccttt cgaaatgctg
     6361 acggtaattt tgtcggtcaa aaggccgata ttctccctgg ttactgttgc catactgttt
     6421 cagttgaaat tgggcggcaa aggtacggat tttttcatta tccgccggcc caaccttccg
     6481 gcgctcctac ctcccgggtg ccggctgtaa ctgcccataa gtcgggattt tttgcatgat
     6541 ctgtgccctg atcatagtca gcaggtagat ctccgcctgg tagaactcga atgccaccac
     6601 cgcattggtc gcagtcttga tctttttata ataggtttcc agcgtacgtt cctggtccat
     6661 gcggttcttg aaatataatt gccccagcga atctgctacc tgaggagtca tccgttgata
     6721 ggatagggcc gtaccttcgt aaagctggag cctatccttt gccgttgccc tgttcccctg
     6781 ctgatattct tcatagatct tccagaatgc ggcggaatct ttcccataca ctggtacgag
     6841 cctcgcgatg gcttcccgct tttgaatacc gaggaggtta atcacggctt ccgcttccgc
     6901 atcggatgtc tgtgcagaca atatcagggg accggccaca gacaaaaggg ccagcagcgc
     6961 aaattttact tgcatagata tagttttccg caagaaagcg attcccgccg aattcgagtg
     7021 ggatttacag taaaacgaag taaaggaccg cttctagcca aaaatgcata tctgaagttt
     7081 atgacttgtt ttgctttcta aggggttggg tgatatatgc cgtcagctcc gcatcttttg
     7141 acggcggcga aagttttggt tttttgggat ccggatcggt atagccaccg cctttgaaat
     7201 aagttccccc tttagtcttc cggggcggcg aatagctgcg cttgttttga ggccgtcttt
     7261 tgccggatgg ggagatagat gaaaaatact cgctgacatt cttgttcacg aagcttcttt
     7321 cagcgggtgc cagggtgata ccatcaatgc gaagtttgac ctcattccgt cgttcttcca
     7381 ggctattcgc ccatgcaacc tccttcgtac ttgaacttcg cggggctttg tcggccagtt
     7441 ccgcaggcgc gacggaagcc agggcaatgg atcctatggc caccgatacc gatcgatcac
     7501 ttaccccctt tgtggaagta gacgtggatg ccattttgaa atttgccttt cggacgaggt
     7561 tatgtgcttg tggttgcccc gtcgtttttt tctcaatgta tccggaatgc cagacggcgg
     7621 tatcgttggc gccggataac aatatggaag tgcttgtccg tgctccatcc gcggaactga
     7681 tcattttttc tgctacagca gtattgactt ttgatccatg gtaggatagc aacgagggat
     7741 tcttattcgt cacctgtagc gtgctggtcc catccgcgtt cgggatggca ttccgtaaca
     7801 gggcatattg gtcgactgga agacctgcct catgcagttt tttaccgcgg aagtccttgg
     7861 gtttcgataa ggttcctgtt gtctttgccg ccttactgaa ttttccggac tgacgctggc
     7921 ttttgttcgc cgtgttatca gtgctaatgg aatattttgg tggcatggct caggtctttt
     7981 cgatggatat cccccgaatt tgatatccgc atcgggcagc accggcgggg tcgatgggtc
     8041 tcagggtgat ctcgatcgta tttactccca gctgcaaata gtcgtgatag tccacggtgg
     8101 cgtaatccag attacggagg tttatggacg acaggttgtt ctccccaccc tcggcttcca
     8161 gcgcatccac atacttgacg atagtcgcat cggtgtcccc attgatgacg atcgacagtt
     8221 ttacaccggt ggaggaggcg tcacccgttt tccaatgctt catgaacaag gtgtattcat
     8281 tccaattgtc cgttatctta aaatatagag tcatggataa tggcaggggt tctgcagaaa
     8341 agttggccag gaggcccgtt tctactgccg tgaaccccag tccttcgtat ccgaactccg
     8401 ggtaagtagt tgtatcgcgg atcaggagat catttgctgt ggaaatgatc ttaaatgcac
     8461 cttttgcccc gtctctattc aatgggaagg gtttcctgag aaatttaccg gggataaacc
     8521 caatcacggg gttccggcct gatacgtcca ccgggtcgag cgccaggagg gccgtgtcaa
     8581 tttttgtttc caatgattcg tcttggggga agcgcagaga tacggcttgc ttgtcgatat
     8641 agactgaaag cccgttactg tccagaaaat gggagcccga aaaatagttg acgacgtgct
     8701 taaaggtcac ttcatcgtct gcatagttgt cgatggtgat cagccaaagc cctttcatta
     8761 caaaattgat cccgaatatc gagagctcac tgggtggata taatactttt tctgtcttgt
     8821 catagagtcg ggcggccatg gcgggggaga cgatgatctc cttttggttg ttcttatcgg
     8881 aatcttttgc tttttccgtc ctgcattgga tggcgtccca ggggtattcc tgcccgaaag
     8941 tccagacaac ggctttattc gtcgggttga ccagggcgta ggatccccaa tccttaatgc
     9001 tcatcataga cgatgaagaa tcttcccggc tctggcttcg cgaataatcc cggccttgcg
     9061 tcgaagattg gtcatgggtt tcggtcgtgg aatgttcgta attggccgac atgctgtcac
     9121 tttgttcagt caggccaacg ctgatggata ctccataact gttggtttcc gaagtagagg
     9181 atccgacggt attgcttacc gatgtaccgt tggtcttgcc gtcagtgtct gctgaactcc
     9241 cggactgctg gatcttagta ttcactgtct gcggagaata ttgtagcaat tggatatcca
     9301 atccttctgc tccatccggt ttttccgggt atatgaatgt atttaccagc acagggtaat
     9361 tctcgaagct tggtgtatcg gaatatggga tggggctgac atccaggcct tgttgattga
     9421 agatatttat cttctgcgtg cagtggatca aatatttctg gcttgtattt tttatgttca
     9481 gggattggaa gtcttttatc tggatatcgt catacttcgt tacctggtat tccacgaaga
     9541 aatttccgat gacgttgggc ttcaacacca ggtcgcggaa tagcgagaag gaatagggat
     9601 ctttcttttt gaacataggg ctattttgta ttgtcgggct cgccgccgta aatgatatag
     9661 ttcactgcat agtaaggttg catggtgttg tgtgcgccgt ctccgcccgt cggcgcgata
     9721 accttgttcg ggtcacccgc ggtagcctgc tgacacatca cattcctgcc gtcgtcgtcc
     9781 ggttgccagg aactgggccc cagtccaaag tcataccatt gtgaactatc gaaaaaatga
     9841 ttgtgcgagg ggatctcggt cgtcaccaat tgatgctgaa actcgccgcc tgtgtccccg
     9901 atttcccagt ctgcgtccgg cgcgaagttg gggaagctgc catcggattg cctgggagcg
     9961 gggtacggtg gagccgcggc gccgatgagt gtccgtccac aaaga
//
LOCUS       DASYSV010000214         9372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1834273, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000214 DASYSV010000000
VERSION     DASYSV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834273"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..641
                     /locus_tag="VGQ51_06565"
     CDS             138..641
                     /locus_tag="VGQ51_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036259.1"
                     /translation="MNDLIFRVTVKTQLAQLNWLITVYLAIGILFYCLFGLSLRPPIA
                     YIYSAVFLFDILPTLLVHVQYYRVNNGAVLDINRELHRISYITPHETLSYNFDDISSF
                     VRVDSWGAGQWYSFAEYRYYKITFKDKKKIIITSLMIKDIKYVLEPMLHMTAVKERRF
                     VAFIRKR"
     gene            1091..1438
                     /locus_tag="VGQ51_06570"
     CDS             1091..1438
                     /locus_tag="VGQ51_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036260.1"
                     /translation="MKILLIGFCLVGLILCTSVAIDKQSGPSIVGLWCLSSSQPNYPT
                     IRFQADSIAEFGSRLDTVYTFKYYLKGGYVNVMTGAGKMYRNKIVKLTDDSLVFQGLL
                     ELKQNQIYHKCKK"
     gene            1970..2554
                     /locus_tag="VGQ51_06575"
     CDS             1970..2554
                     /locus_tag="VGQ51_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036261.1"
                     /translation="MKKRRFLILTLISPLLLMACQTKRKEKVKGARDISIGELSIELP
                     KSYHFIQERGIDSYAAYIVNGRNDSFQIDYGNPRTIFRLFERPPVAWPTTLRTRTMQA
                     SAKAANPDNVVYSKTWQEDNEEGIFMENYHLYDTINGLTVKFVLPKRPGKGMTGMLVL
                     DLPDSNCMSIYARNLDTASQDSALAFFRTVRLKE"
     gene            complement(2615..2929)
                     /locus_tag="VGQ51_06580"
     CDS             complement(2615..2929)
                     /locus_tag="VGQ51_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036262.1"
                     /translation="MPTSRIATLSSGEFVGITADSPDQLMPLKAVHCQVQLEKQPVHK
                     PISQPTKLISKEIVNNTFLPIQQEAKATVGNRLDHMRTNPDLNKLILNPKVGARRKQN
                     KL"
     gene            3302..4135
                     /locus_tag="VGQ51_06585"
     CDS             3302..4135
                     /locus_tag="VGQ51_06585"
                     /inference="COORDINATES: protein motif:HMM:NF020914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RteC domain-containing protein"
                     /protein_id="HEV9036263.1"
                     /translation="MMKPFDALYADLEKSLKLAGGSQPGPDVYLRGIGTIREKIAKLR
                     AQGARVIQNVGAKEREFFRDVWPPFHAKWLLYIRLYEMELGRRSMPADAWPTVIAMEE
                     KRVEGFFRRNRAFWLYYRSGGPAIVEQFSRAYSQGRIFHPLAMIMDPEWVTLASWRAA
                     WCLAMQSYGEWLREERAVMSVGAVSAADLGYSWAASDANLAEWLFALQAVGAVHYMGQ
                     PADIGRLQKWARVALGREVANIYDRGRVLRKRKKDQLAFITRTTVALQRKWNQAKGDI
                     E"
     gene            4305..4856
                     /locus_tag="VGQ51_06590"
     CDS             4305..4856
                     /locus_tag="VGQ51_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036264.1"
                     /translation="MLKGITWVQFSIFILVAASLYYLYVAAVFYRAGVLRTRRKGEPA
                     PGQNTREETIRKENGAEEATTRLPAKSQEEAPGRVGQGGMEGKAALPRNVKEKEAVAA
                     TDDLPQLALKAIATIRQVIAQGTENKHDRQNVMDHIGEVLTDYRKLRRTEHAETINNF
                     LIRVCASDLSLHLSEKELAELWK"
     gene            4904..5221
                     /locus_tag="VGQ51_06595"
     CDS             4904..5221
                     /locus_tag="VGQ51_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4134 domain-containing protein"
                     /protein_id="HEV9036265.1"
                     /translation="MRGRISWRKWALLAGGLAAQVSAMAQTADGNTGLSQANTMVRGY
                     YDTATNLMFAIGAILGIVGAIRVFSMWIRGDERMGHTAAMWFGACIFLVVVATVIKSF
                     FGL"
     gene            5232..5573
                     /locus_tag="VGQ51_06600"
     CDS             5232..5573
                     /locus_tag="VGQ51_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4133 domain-containing protein"
                     /protein_id="HEV9036266.1"
                     /translation="MAAKIFQINKGIGRPVEFRGLKAQFIGYLAACVVSTMMGFGVLY
                     VCGMSLYVATPSALGLGGWMISVVFRMSRRYGQYGLMKRSAKKRMPEALLSRSRKMFI
                     QLRSDYVRTIG"
     gene            5554..8022
                     /locus_tag="VGQ51_06605"
     CDS             5554..8022
                     /locus_tag="VGQ51_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032991 - protein-containing complex
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraG family conjugative transposon ATPase"
                     /protein_id="HEV9036267.1"
                     /translation="MYGQLDDRLPIYEVADDWIVNKLGDVCIGLEVEKPEIFTLGEND
                     YDNLHLAFVKAIRVLPAGTVLHVQDIYRRGKYKATALRADAEFLDRASEQFFKGRQFL
                     RHRCRMYLIRRPAGRLKVTSASSGILRKTLVPADTLDEKICREFESAVRQFCHILEDS
                     KLIRSRRLRTEELVSSSKKAGVIEQSCYLLEEDETPVIRDIGLQDRVCIGDKEVVTYT
                     LGDAERLPGQCSTWVPYEPYSTERTRYPVSFAAGLGLLLPFDHIYNQYIVVEDVAATM
                     KRLESRRLQLQSLSRYSRENLLARDAVNVFLTEAITQGYRPVRFCANVMAWTEEPGQL
                     AEINHKIGAAMAAMDATAHQETVGAPQLWWASVPGNGGDIPDNECCDIFLEQAACFLG
                     MESNYADLGKEYGIRLGDRLTGKPVMVDIDIEPRHRGWTGNGNLFVLSGSGGGKSFLM
                     AHLCRTYHAQGMHIVLVDVGRSYETLCRLLGGVYKTYEEDAPIAFNPFYIEKGQQPDI
                     EKKESIKALVLALWKRADEDHYRSEYVTISDALAAYYMMVADKPEVFPCFDSFYEYME
                     GDYCRHLRSRGVRDTVFDIDNFLYVLRPYYRGGEFDFLLNAREDLDLLSRRFIVFELK
                     NIVGHPILFTVVTMVIVEVFMAKVRTLRGTRKMIVIEEAWKAIASAGMADEIRGWVKT
                     LRKHMGKLALVSQELEDIVDSPIIKQAVINSSDCKILLDQSKFSNRFAEIQAMLGLTD
                     KQKTEVLSINKGHDPGRPYKDCWIGLGPSWSRVYRLETSEEEYLAYTSDEGDKLKIEE
                     YEHRHGGLQEGIEKLADELRTGRG"
     gene            8084..8713
                     /locus_tag="VGQ51_06610"
     CDS             8084..8713
                     /locus_tag="VGQ51_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraI"
                     /protein_id="HEV9036268.1"
                     /translation="MPSRSDAQVLDIINIINAAVKKVIVATDLEIERLQTETIDAQNA
                     GKALENDMEQSELSGIASWVQQQRDLFSEYYQELKQVKNVIFSYEEVKEMIDKQVKII
                     AGYQQVYSALRQDKHFSLDEVSHALAVLAGIASQSAQNIQRLTLVVTSLLTQISDAGR
                     LRLIDETGSDIDRNYSDLVEFSQQSFLLSLQRARDADDIAVTRALYGIQ"
     gene            8736..>9372
                     /locus_tag="VGQ51_06615"
     CDS             8736..>9372
                     /locus_tag="VGQ51_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036269.1"
                     /translation="MKKTIFKKIVLGILGAAMVKAVRAQTFAEWFQQNSTRLKYYAGQ
                     VAALQAYLGQMQQGFQITSSGLGDIGSLKNDEFALHSNQYSSLTTVNPAVSSMGEVME
                     IVALARASSTRLISAMNRYRRDGMLTTDQLNLVRELFKTAIGAELQDVEMLKTLLTNN
                     VLQLTDDQRVSRVLAIHTSVRQRYGFVLSMTDRIDLFERQLSEALAQMATVE"
BASE COUNT         2388 a         1921 c         2808 g         2255 t
ORIGIN      
        1 gcggtggatc ccgagaaggt gatcctgttc ggcagtcatg cgacggggag atgggtggag
       61 caccggtaca cggagggcgg catcacttga ttctcacatc gggaaaattg aattaaagag
      121 aatatactta ttcttacatg aatgacttga ttttcagagt gaccgttaaa actcagttgg
      181 cccagttgaa ctggctgatt acggtatatt tagcgattgg aattttgttt tattgtttat
      241 ttgggctttc tttgcgaccg cccattgctt acatatattc agcggtattc ttgttcgata
      301 ttttgccaac attgctcgtg catgttcaat attatcgagt aaataacggc gctgtcctgg
      361 atataaaccg ggaacttcat agaatcagtt atataactcc tcacgagacg ctgagctata
      421 actttgatga tatcagctct tttgttcgcg tggattcatg gggtgctggc caatggtatt
      481 cattcgctga ataccgatat tataaaatca cgtttaagga taagaaaaaa attatcatta
      541 ctagtttgat gatcaaggat atcaagtatg tgctggagcc catgttgcac atgacggccg
      601 ttaaagagcg aaggtttgtt gcgtttatta ggaagaggta gagccacgaa cttggcgttc
      661 aatttattta aagggacaaa ttatctcaat tccggaacag ataaaagttt gggaagtttt
      721 aatggaaaca gatcaattca cgtactaagg cgaaattgat gatgggcgaa aattcctacc
      781 aaataagaaa taattagtga tgcatgtatg gaaatgcaat aaacgactta cttaatagat
      841 gagcatttag atcagatata ttatttatta gcccagaggg gaacggctgt agatgctccg
      901 tatacatatg ctccatcaca gggagcaact cagattaatg ccgccactgg tcaaagaacg
      961 aatacgatga atccgttgac aaggagagtt acccaggctg atattgataa tttgggctta
     1021 caaaatctaa cttatgatcc agtaacagga aagggatcgt taggtgcttt aacgaacaaa
     1081 taccactaat atgaaaatcc tgctaattgg cttttgcctt gtcggtttaa tattgtgtac
     1141 gtcggtggct attgataagc aaagtggccc ttcaatagtt ggcttgtggt gtttaagcag
     1201 tagccaacct aattatccga caataagatt tcaagctgat tcaattgcag aatttggaag
     1261 tagattggat acggtctata ccttcaaata ttatttgaaa ggaggatatg taaatgttat
     1321 gacaggtgct gggaaaatgt atagaaataa aatagttaag ctaacggacg acagtctggt
     1381 atttcaaggc ttgcttgagt taaagcagaa tcaaatatat cataaatgta aaaaatagca
     1441 agtctaatcc tcctcattga cgaggatcaa tattatctgt ttgggctgag gataggggat
     1501 atcgtatatt tgtcaaccgt tagtttttat gacgatcact cacggctgat ccaagggcag
     1561 acagtcaact atacagggag cgttgacact gtaaccactc aatatgattt tactggcaag
     1621 ccgttacggt ctcttttggc tcaagccaaa ttgggtaata ctgcccagac tcacggggtt
     1681 tcaacaaaaa cgaccaatga cccgaatttc agaacaacca gtatctggaa gaatattgac
     1741 gcggctgttg tcgataagtt gatcgaccgt atgaaataca atgagctcgg tcagttgaaa
     1801 aaaagtatct cggcaagcgt cctgtgacgg gaatgccgat agatagcttt gaatacgact
     1861 acaacatacg cggatggatg actgggatca ttatgccagc cggagattac ttcaattctt
     1921 ttctgaatcc tgaagatact cgtaaacagc tgaaagaatt tattctaata tgaagaagag
     1981 aagattttta atattgacgc tgattagtcc gttgcttctg atggcctgcc aaacaaaaag
     2041 gaaagaaaag gttaaaggag cgagagatat ctcaataggg gaactatcga tcgaactacc
     2101 aaagtcttat cattttatac aagagcgagg gatcgatagt tatgctgcat atattgtaaa
     2161 cggccggaac gattcgtttc aaatcgatta tggcaatcca agaacaatct ttagactttt
     2221 tgaacgacct ccagtcgcat ggccaacgac attaaggact cggactatgc aagcctctgc
     2281 aaaggccgca aacccagata acgttgttta tagtaaaact tggcaagaag ataacgaaga
     2341 aggcattttt atggaaaact accatttgta cgacaccata aatgggctta cggttaagtt
     2401 tgttctgcca aaaagacccg gcaaaggtat gacaggaatg ttggtattgg atctgcctga
     2461 cagtaattgc atgagtattt atgcaaggaa cctagatact gcaagccaag actctgctct
     2521 cgcatttttt agaactgtca ggcttaaaga gtaataacca atagcctagg tgtaaaatcg
     2581 aaatggcgag aagcagattc taagttgcga tacgttataa tttattctgt ttgcggcggg
     2641 cgccgacttt gggattaagg attagtttgt tgaggtcggg gtttgtgcgc atgtgatcga
     2701 ggcggttgcc gacggtcgcc ttagcttcct gctgaatggg caggaaggtg ttgttgacaa
     2761 tttctttgga aatgagtttg gttggctgtg aaatgggttt gtgcactggc tgcttttcca
     2821 gctgcacctg gcaatgaacg gccttgagag gcatgagctg gtcgggactg tcggcggtga
     2881 tgccgacgaa ttcgccactc gatagggtgg cgatcctgct ggtcggcaag ggccggggct
     2941 attgcaggct ttggctggtg gaagcgccgc cgcctactgg cgcgcaatgg aatgacattg
     3001 gaagagattg catttttggc gggtgttact gtggccgaag taaaaaaggt gaaggcgcaa
     3061 agtaaagcga aatagattcc agaatgaata ttgaaaccgt ccggatcatt cgggcggctt
     3121 ctttgtttat gttcaactag cggcatatgt acttatgcgt agctcaagtg ggtatcggcc
     3181 tacgtacttg ttgggggtat tattgcgaat ttttcatcct ttttggggtt tcgtgtggct
     3241 gaatgcccga agatcggcgg gctattctag cttcgagggc ataaccttaa agtgtccagt
     3301 catgatgaag cccttcgatg cattgtatgc cgatctggag aaaagcctga agctggcggg
     3361 cgggagccag ccggggcccg atgtctatct ccgtgggatc gggacgattc gggaaaaaat
     3421 tgcaaagctc cgcgcccagg gcgccagggt gatccagaac gtcggggcaa aggaacgcga
     3481 attcttccgc gacgtttggc cgccatttca tgcgaagtgg ttgctgtata tccgccttta
     3541 cgaaatggaa ctgggccggc gttcgatgcc ggcggatgcg tggccgacgg taattgccat
     3601 ggaagagaaa agggtagaag gctttttccg gcgcaatcgg gcattctggc tgtattaccg
     3661 cagcgggggc ccggctatcg ttgagcaatt cagccgtgca tatagccagg gcaggatctt
     3721 ccatccgctc gctatgatca tggatccgga atgggtgacg ctggctagct ggcgggcggc
     3781 atggtgtctg gcgatgcaga gttatggcga gtggctgagg gaggagcggg ccgtgatgtc
     3841 ggtcggcgcg gtttccgcgg cggacctggg ctatagctgg gcggcctccg atgcgaacct
     3901 ggcggaatgg ctcttcgcct tgcaagcggt gggtgccgtt cactatatgg gacagcccgc
     3961 agacatcggc cggcttcaga aatgggccag ggtggcgctg ggccgcgagg tggcgaatat
     4021 ctacgaccgc gggcgggtgc tccgcaaacg caaaaaggat cagctggcct ttattaccag
     4081 gacgacggtc gcgctgcaaa ggaaatggaa tcaggcgaaa ggggacatcg aataagtcgg
     4141 cgagcgcgac tggcgatgtt ttcaaagtct attttaagca ttcggataaa agtttttcat
     4201 attggatgcg actttcgaat gaaaatcaat agaggcatta gtgcctctta tttttttgta
     4261 ggccgggact acccattttt gttctgcgat caaaacgcat gggtatgctg aaaggaatca
     4321 cttgggtgca gttttcaatt ttcattttgg tggcggcgag cctgtattat ttatatgtgg
     4381 ctgccgtctt ttatagagct ggcgttttgc ggacaaggcg gaagggtgag ccggctcctg
     4441 gacagaatac gcgagaggaa acgataagga aggagaatgg tgcggaggag gcaacgacaa
     4501 gattgcccgc gaagtcgcag gaagaggcgc caggcagggt cggacagggc ggaatggagg
     4561 gaaaggctgc gttaccgcgc aacgtaaagg aaaaagaggc cgtcgccgca acggatgatc
     4621 tcccgcagct ggcgctgaag gcaattgcta ccattcggca ggtgatcgct caggggaccg
     4681 agaataaaca cgatcggcag aacgtgatgg accatatagg agaagtgctc acggactatc
     4741 ggaagctccg caggacggag catgccgaaa ccatcaacaa ttttctgata cgggtatgcg
     4801 cgagcgacct ttcactgcat ctgagcgaga aggaactggc ggagttgtgg aagtagagca
     4861 aaatggataa agcggcgatt tcttaatcta aacataagtg attatgaggg gcaggatctc
     4921 gtggagaaaa tgggccttgc tggcgggcgg cctcgcggcg caagtgtcgg cgatggcgca
     4981 aacggcggat ggaaatacag ggctgagtca ggccaatacg atggttcgag ggtactatga
     5041 tacggcgacg aacttaatgt tcgcgatcgg tgctatcctg gggatcgtcg gcgcgatccg
     5101 ggtattctcg atgtggatac gcggcgacga gcgcatgggc catacagcgg caatgtggtt
     5161 cggggcgtgc atcttcctcg tggtggtggc aacagtcatc aagtcatttt ttgggctgta
     5221 gtagcaagac aatggcggcg aaaatcttcc aaataaataa gggcatcgga aggcctgtcg
     5281 agttccgggg tttgaaggcg caattcatcg gctatctggc ggcgtgtgtg gtgagtacca
     5341 tgatgggttt cggggttttg tacgtctgcg ggatgagtct gtatgtagca acgccttcgg
     5401 ccctgggcct cggtgggtgg atgatcagcg tggtcttccg gatgagcaga cggtacggcc
     5461 agtacgggct gatgaaacgg agcgcgaaga aacggatgcc agaggctttg ctgtcgagat
     5521 cgagaaagat gtttattcaa ctaagaagtg attatgtacg gacaattgga tgatcgcttg
     5581 ccgatctatg aggtggcaga tgactggatc gtgaacaaac tgggggacgt ctgtatcggg
     5641 ctggaggtcg aaaaaccgga gatctttacg ctgggcgaaa acgactatga taatctgcac
     5701 ctggctttcg tgaaggccat ccgggttttg cccgcgggaa cggtgctgca tgtacaggac
     5761 atctaccggc ggggcaaata caaggcgacg gcattgcggg ccgacgcgga atttctggac
     5821 cgggccagcg agcagttctt taaggggagg caattcctgc ggcatcggtg ccggatgtac
     5881 ctgatccgga gaccggcggg gaggttaaag gtgacttctg cgagttcggg catcttgcga
     5941 aaaacgctgg tgcctgccga tacgctggat gaaaagatct gccgggagtt cgagtcggct
     6001 gtacggcaat tctgccatat cctcgaagat tcaaagctga tccgttcccg acgactcagg
     6061 actgaggagt tggtgagcag tagcaagaag gcgggtgtca tcgagcagtc ttgctacctg
     6121 ctggaagaag acgagacgcc ggtgatccgg gatatcggcc tgcaggatcg ggtttgcatt
     6181 ggggacaagg aggtagtgac ctatacgctg ggtgatgcgg agcgcttgcc gggacagtgt
     6241 tcaacatggg taccctatga gccctatagt accgaacgga ccaggtatcc ggtaagcttc
     6301 gcggctggtt tgggattact gctgcctttc gatcatatct ataatcaata catcgtggtg
     6361 gaggacgtcg cggcgacgat gaagcgtctg gaaagcaggc ggctgcagct gcaatcgctg
     6421 tcgaggtatt cgagggagaa cttgctggcg cgtgatgcgg tcaacgtttt tctgacggag
     6481 gcgataacgc agggctatcg gccggtacgg ttttgcgcca atgtgatggc ctggacggag
     6541 gagccggggc aactggcgga gatcaatcat aaaattggtg cggcgatggc cgctatggat
     6601 gccacggcgc atcaggaaac agttggagca ccacagctct ggtgggcgtc ggtacctggt
     6661 aatggcggcg atattcccga caacgaatgc tgtgatatct ttttggagca ggcggcttgt
     6721 ttcctgggca tggaaagtaa ctatgccgat ctcggcaagg agtacggcat tcgtctgggc
     6781 gaccgtctga cgggtaagcc ggtgatggtg gatatcgata tcgagccgcg gcaccgcggg
     6841 tggacgggga atgggaacct gttcgttctg tcgggcagcg gcggcgggaa gtccttcctg
     6901 atggctcatt tgtgccggac ctatcatgcg caggggatgc atattgtgct ggtagatgtc
     6961 gggcgatcgt acgagacgct ttgccggctg ctcggcgggg tatataagac ctatgaggag
     7021 gatgctccga tcgcgttcaa tcccttctat attgaaaagg ggcagcagcc ggatatcgaa
     7081 aagaaggaga gcatcaaggc gctggtgctc gcgctgtgga aacgcgcgga tgaggatcat
     7141 taccggtcag agtatgtgac gatctccgat gccctggcgg cctattatat gatggtggcg
     7201 gacaagccgg aggtcttccc ctgttttgat tcgttctatg agtacatgga gggcgactat
     7261 tgccggcatt tgcggtcccg gggggtgcgg gacacggtat tcgatattga caacttccta
     7321 tacgtgttac ggccttatta tcggggaggg gaattcgatt ttctgctcaa cgcacgggaa
     7381 gatctggacc ttttatcgcg acgctttatc gtattcgagt tgaaaaatat tgtcgggcac
     7441 ccgatccttt tcacggtggt gacgatggtg atcgtcgagg tcttcatggc gaaggtgcgg
     7501 acgctgcggg ggacgcggaa gatgatcgtg atcgaggaag cctggaaggc gatcgcgagc
     7561 gcgggtatgg cggatgagat tcggggctgg gtaaagacgc ttcggaagca tatgggcaag
     7621 ttggcgctgg tgtctcagga attggaagat attgtcgatt cgccgatcat caaacaggcg
     7681 gtcataaata gcagcgactg caagatattg ctcgaccaga gcaagttttc caaccgtttt
     7741 gccgagatcc aggcgatgct ggggctcacc gataaacaga agaccgaagt cctttccatc
     7801 aacaaggggc atgatccggg aagaccgtac aaggactgct ggatagggct gggaccgtcc
     7861 tggtcgaggg tctatcggct ggagacatcc gaagaggaat acttggctta tacatcagac
     7921 gagggggata aactaaagat cgaagaatat gagcatagac atggtggttt gcaggaagga
     7981 attgaaaagt tggcggacga actacggacg gggagaggat agggcatgca gaggagggca
     8041 tcggattttt ctggttttac cggtgttcct tctctcctgc ctaatgccgt cccggagcga
     8101 tgcgcaggta ttggacatta tcaatatcat caacgccgcg gtgaaaaagg tgatcgtggc
     8161 caccgacctg gagatcgagc gattgcaaac cgagacgatc gatgcgcaaa acgccgggaa
     8221 ggcgctggaa aacgacatgg aacagtccga gctgagcggg attgcctcct gggtccagca
     8281 acagcgggac ctgttctcgg aatactacca ggaactaaag caggtgaaaa atgtgatctt
     8341 ttcctatgag gaagtaaagg aaatgatcga caaacaggtg aagattattg ctggatatca
     8401 gcaggtctat tcagcactac ggcaggataa acatttcagt ctggatgagg ttagccacgc
     8461 attggcggtg ctggcgggta tcgccagcca gagtgcgcag aacattcaac ggctgacact
     8521 ggtggtgacg tccctgttga cgcagataag tgatgcgggg aggctacggc tgatcgacga
     8581 gacgggcagc gacattgacc ggaattacag cgatctggtg gagttcagtc agcagagttt
     8641 ccttttgagc ctgcagcggg caagagatgc ggatgatatc gcggtgacga gggctttgta
     8701 tggaattcag taattggata aaaaaattat cggcgatgaa aaagactatt ttcaaaaaga
     8761 tcgttctggg aatattaggt gcggcaatgg tgaaagccgt gcgggcacag acgttcgctg
     8821 aatggttcca gcagaatagt acccgattga aatactacgc cgggcaggtg gccgcgctgc
     8881 aagcctatct gggacagatg caacagggat ttcagataac ctcctctggg cttggtgata
     8941 tcggtagcct taaaaacgat gaattcgcgc ttcattccaa tcagtattct tcgttgacga
     9001 ccgtcaatcc tgcagtgtcg tcgatgggcg aggtaatgga gatcgtagcg ttagcgagag
     9061 ccagttcgac aaggctcatc tccgccatga accgttaccg gcgcgacggc atgctgacca
     9121 cggaccaact gaacctggtg cgggaacttt ttaagacggc tatcggggca gaactacaag
     9181 acgtggagat gctgaagact ttgcttacca ataatgtatt acagttaacc gatgaccagc
     9241 gggtgagcag ggtattggca atccatacgt ccgttagaca gcggtatggg tttgttttat
     9301 cgatgacgga tcggatcgat ctgttcgaaa gacagctgtc ggaggcgttg gcgcagatgg
     9361 cgacggtgga aa
//
LOCUS       DASYSV010000215         6947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_164703, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000215 DASYSV010000000
VERSION     DASYSV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164703"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /gene="gcvH"
                     /locus_tag="VGQ51_06620"
     CDS             complement(<1..296)
                     /gene="gcvH"
                     /locus_tag="VGQ51_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEV9036270.1"
                     /translation="MNFPANLRYTKDHEWIRLDKDNEAVIGITDFAQKELGDIVYVEV
                     ETVGKALAAETVFGTVEAVKTVSDLYLPIAGTILEVNPELTNAPDLVNSDPYGKG"
     gene            complement(378..1085)
                     /locus_tag="VGQ51_06625"
     CDS             complement(378..1085)
                     /locus_tag="VGQ51_06625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV9036271.1"
                     /translation="MIKRLLAGGFLLAMISCNPKLSNGLRKNDLHRDVEIITTKGTMV
                     IRLSDSTPQHRDNFLRLAKTGFYDSLLFHRVIRNFMIQTGDPDSREGHPGKPMSQGGS
                     GGPGYTIPAEFRQTLFHHRGAVGAARNNDDVNPTRASSGSQFYIVQGRRFTQAGLDSV
                     ETFRLKGRKIPADERKVYEEAGGSPHLDQAYTVFGEVISGMEVVDSIASAPTTGRPLD
                     RPISDIRILKMKLIRRK"
     gene            complement(1082..1450)
                     /locus_tag="VGQ51_06630"
     CDS             complement(1082..1450)
                     /locus_tag="VGQ51_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036272.1"
                     /translation="MQQLQDDLQARWWDLEATLTQRFGKKPDMETILFLIGVQEFGDV
                     RRKFTKEEKQDLMHIAVCSLFGQSGYYEIERVDEEGWPHFRQLKALPVMSLIEQENFL
                     KDHILLYFGKDSTESELNNL"
     gene            complement(1519..1851)
                     /locus_tag="VGQ51_06635"
     CDS             complement(1519..1851)
                     /locus_tag="VGQ51_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEV9036273.1"
                     /translation="MLDPTMITTSTALEGHRVLRNLGVVRGITVRSRSLLGNLAGGLQ
                     TLLGGQISVYVDLCEKTREEAFQYMMQHAAERGANAILNMRYDANEVMNGVTEVLAYG
                     TAVLVEKV"
     gene            1995..3266
                     /gene="serS"
                     /locus_tag="VGQ51_06640"
     CDS             1995..3266
                     /gene="serS"
                     /locus_tag="VGQ51_06640"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV9036274.1"
                     /translation="MLQVAFIRQNVDFVKERLAIRNFTETALVDDIVRLDDQRRKLQL
                     ESDTTLSAINALSKEIGQLMAKGQKGEAEAKKQEVATLKASQAPIADQLAAVEKQLNA
                     AVVLLPNLPAAAVSPGRSAEDNVTIREGGHRPALSKTALPHWELAKKYDLIDFELGNK
                     ITGSGFPVYIKKGAKLQRALIQYFLDFNTAAGYSEYQPPILVNEASARGTGQLPDKEG
                     QMYFVNEDNLYLIPTAEVPVTNIYRDEILKESQLPIKMTAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIVDPSKSYEVLEEMVLHVERLLQSLGLPYRILRLCGGDMGFTSA
                     MTYDFEVYSAAQDKWLEVSSVSNFESFQTNRMKIRFKDASGKSVLAHSLNGSSLALPR
                     IVACLLEDHQADGYIALPQPLHTYFGADRIA"
     gene            3371..4033
                     /locus_tag="VGQ51_06645"
     CDS             3371..4033
                     /locus_tag="VGQ51_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036275.1"
                     /translation="MKRIYSVFACIVICSVAQAQVGKGAIIIGGNIAYQDQRSDNPSA
                     TYLTHDLNINPSIGKAIRTNLVLGIDLSYSHSISDYMNAGEPNDTQAGNSFYAGVFLR
                     RYKLLGNGFYLFGQAEAGINYGHTSTDYPLNVPPSSDKTNSYGIQLLLYPGIAYAINQ
                     HWQIETGLPNFFAVHYNHSSETAVVTGQSSQESKSHSLYISSSLGGTNAFTVGVRYFI
                     GG"
     gene            complement(4073..4276)
                     /locus_tag="VGQ51_06650"
     CDS             complement(4073..4276)
                     /locus_tag="VGQ51_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036276.1"
                     /translation="MSTVTLTAYNVKTKEKNVPIQDAVITKTAKGAYMAQGHDGKGNK
                     LTTLLGEEKALSAIKAGIAKQGW"
     gene            complement(4313..6292)
                     /gene="gyrB"
                     /locus_tag="VGQ51_06655"
     CDS             complement(4313..6292)
                     /gene="gyrB"
                     /locus_tag="VGQ51_06655"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV9036277.1"
                     /translation="MSETTQDVLTAASNGYGADSIQVLEGLEAVRKRPAMYIGDISEK
                     GLHHLVYEVVDNSIDEALAGYCKNIEVTIFEDNSIKVEDDGRGIPTGINTKEKKSALE
                     LVMTILHAGGKFDKNTYKVSGGLHGVGVSCVNALSSDLHVTVFREGKVFEQEYKIGVP
                     QYPVREIGKSDKTGTTTHFWPDTSIFTSTVYKKDILEGRLRELAYLNRGIRIALLDLR
                     EKEEDGSPYSKTFYSEGGIVEFVEMLDKNAGRSPLIPKIIYVEGRDEVTNVAVEVALT
                     YNDSYSEHIYSYVNNINTIEGGTHVAGFRRALTRVFKAYGDKQGLFERAKVEIEGDDF
                     REGLSTIVSVKVPEPQFEGQTKTKLGNSEVSGVVDSTVSRMLDAYLEENPRDAKNIIA
                     KVVLAAQARAAAKKARELVQRKSVLSGGGLPGKLADCSDRNPDRCELFLVEGDSAGGT
                     AKQGRERSFQAILPLRGKILNVEKAMEHKIYENEEIRNMYTALGVTVGTPEDPKALNL
                     AKLRYHKLIIMTDADVDGSHIATLILTFIYRYMKEMVEQGYVYIAQPPLYLVKKGKEQ
                     SYAWNEDQRRGLIEKLGGGKEDSVTVQRYKGLGEMNSDQLWDTTMNPNTRTLKQVTIE
                     SAAEADRIFSMLMGDEVAPRREFIESHAKYAKLDV"
     gene            6395..>6947
                     /locus_tag="VGQ51_06660"
     CDS             6395..>6947
                     /locus_tag="VGQ51_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9036278.1"
                     /translation="MFQAPDILTMAYKQPVMEDQMVLLLESQRQIPGSVQYSIKRYAK
                     HPQWTVEDLGMMVYHYKREDPSQNYLELRFCFAGNTYCRRSANSRSGGVVMIPGEAEC
                     DSCRLENGSGCSEKIATVDVFNFRFLPVHLSQFVRPRKPATSVSENLLEFRHTASFSK
                     MMSLCGRTRLVLETLLNHNYTGSH"
BASE COUNT         1547 a         1886 c         1696 g         1818 t
ORIGIN      
        1 cctttcccat aagggtccga gtttaccagg tcgggggcgt tcgtcaactc gggatttacc
       61 tcgagaatag tgcccgcaat gggaagatag agatcggata ccgttttaac ggcctccacc
      121 gttccgaaaa ccgtttctgc agccagcgcc ttccccacag tttcgacttc tacgtatacg
      181 atgtccccca attctttctg agcgaaatcc gtaattccga taaccgcctc gttatccttg
      241 tccaggcgaa tccattcgtg atcctttgtg taacgcaaat tagcagggaa gttcatggta
      301 actaattttt tatgctgcaa agattggaaa aaggccggga aattagccag ccttccggag
      361 aattatttgc ccgaatctta ctttcggcgg atgagtttca ttttcagtat tctaatgtct
      421 gatatcggtc ggtccagcgg tctgccggtg gtaggcgccg acgcaattga atctacgacc
      481 tccatgccgc tgatcacttc tccaaacacg gtataagcct gatcgagatg tggtgaccca
      541 ccggcctctt cgtatacttt tcgctcatcg gccggtattt tcctgccttt cagccggaag
      601 gtctccacgg agtccagccc ggcttgcgtg aatcgtcgcc cctggacgat gtagaactga
      661 cttccgctgc tggcgcgggt agggttcaca tcgtcattat tccgggcggc tcccacagcc
      721 ccgcgatgat ggaaaagggt ttgtctgaat tctgccggta ttgtataacc ggggcctccg
      781 gagccgccct ggctcatcgg tttgccggga tgaccttccc tgctgtcggg atcccctgtc
      841 tgtatcatga agttccggat gaccctatgg aaaagcaggc tgtcatagaa accggttttg
      901 gccagccgca gaaaattatc gcggtgctgc ggagtggaat ccgacagacg gatgaccatg
      961 gtgcccttgg tggtgatgat ttcgacgtcc cgatgcaaat cgttctttct caatccattg
     1021 gatagtttgg ggttacagga gatcatggcg agcaaaaacc ctcctgccag cagtcttttt
     1081 atcataagtt gttcagttca gattcggtcg agtccttccc gaaatacagc aggatgtgat
     1141 ccttcagaaa attctcctgt tcgatcagac tcatgacggg caaggccttc agctgtcgga
     1201 aatgcggcca tccctcctca tcgacccgtt cgatctcgta ataaccgctt tgcccgaata
     1261 gcgaacagac cgcgatatgc atgagatcct gtttttcttc cttggtaaat ttcctgcgta
     1321 cgtctccgaa ctcctgcaca ccgatgagaa ataaaatggt ttccatatcg ggttttttcc
     1381 cgaaccgttg tgtcagcgtg gcttccagat cccaccaacg cgcctgcaaa tcgtcttgta
     1441 gctgctgcat gacgcaaaga taaggaattc ggtcgcccgg tatccggtgc tcccctgttc
     1501 ccctgcatcc gcaacctatc agaccttctc gaccaacacc gcagttccat aagccagcac
     1561 ctcggtcacc ccattcatca cttcgttcgc atcgtaacgc atattgagaa tggcattggc
     1621 gcctctttcc gccgcatgtt gcatcatgta ttgaaatgcc tcttcccttg tcttttcaca
     1681 aaggtctaca tatactgata tttgtccgcc gagcaatgtt tgcagtccgc cggcaagatt
     1741 gcccagcagg cttcgggatc gtactgtaat accgcggacg acgcccaggt tacgcaggac
     1801 acgatgtcct tcgagtgcag tgctggtggt gatcatggtt gggtcgagca tataaagatt
     1861 tttatagtga aatttacagc tttcgggtca aaaccggatg gaatccggtt caaagtcatt
     1921 agcgggtccc gggcaaaaaa ttatttttcg gcaatttttt gtccgggtga tacctttggc
     1981 ccaaaattga gaaaatgtta caggtggcat tcatccgtca aaatgtggac tttgtaaagg
     2041 aaagattggc tataaggaat ttcaccgaaa cggcacttgt ggacgacatc gtccgtctcg
     2101 acgatcagcg cagaaagctg cagctcgaat ccgataccac cctgtcggca ataaatgctt
     2161 tgtccaaaga gatcggtcag ctcatggcaa agggccaaaa aggagaggcc gaagcaaaaa
     2221 agcaggaagt ggcgacgctc aaggcctcgc aggcgccgat cgcggaccaa cttgcggcag
     2281 tagagaagca gctgaatgct gccgtcgtac tgcttcccaa tctgcctgcc gccgctgttt
     2341 ctcccggaag gtccgccgaa gacaatgtga ccatccgtga aggcggccac cggccggcgt
     2401 tatccaaaac ggcgcttcct cactgggagc ttgcaaaaaa atacgatctg atcgatttcg
     2461 aactgggcaa caagatcacc ggcagcgggt tcccggtata catcaaaaaa ggtgcaaaac
     2521 tgcagcgtgc ccttattcag tacttcctcg atttcaatac ggctgccggc tattcggaat
     2581 atcagcctcc cattctcgtt aacgaggcct cggcacgcgg cacgggccag ctgcctgata
     2641 aagaaggcca aatgtatttt gtaaatgaag ataacctgta tctgatcccc accgccgaag
     2701 tgccggtaac gaatatctat cgtgatgaaa tcctgaagga gtcccagctt cctataaaga
     2761 tgacggccta tactccctgc ttccggcggg aagcaggcag ctacggtaag gatgtccgtg
     2821 gtctcaaccg gcttcaccag ttcgacaagg tggagatcgt ccagatcgtc gatccttcca
     2881 aaagctatga agttctggaa gaaatggtac tccatgtcga aagacttttg caatccctcg
     2941 ggcttccata ccgcatcctt cgtttgtgcg gcggcgatat gggtttcacc tcggccatga
     3001 cctacgattt cgaggtctat agcgctgccc aggataaatg gctggaggtg agctcggtca
     3061 gcaatttcga atctttccag actaatcgaa tgaaaatccg cttcaaagat gcctccggta
     3121 agtcagtact tgctcattcg ctcaatggca gctcgcttgc gcttccccgc atcgtggctt
     3181 gtctgctgga ggatcaccag gcagacggat atattgcgct tccgcagccg ctgcatacat
     3241 attttggcgc ggacaggata gcttaaactt tgattaaagc aggcttaaac tttgattaaa
     3301 ccgggcattg gtgcttgcac atgccgggct ttcttttcat cttacggcat ctgcaaactc
     3361 aaactttttt atgaagagga tctattccgt gttcgcttgc atcgtaattt gttcggtcgc
     3421 acaggcccag gttggtaaag gtgccatcat tatcgggggt aatattgctt atcaggacca
     3481 acgctccgat aacccttcag ccacctacct cacccacgat ttgaatatca acccctccat
     3541 cgggaaggcg ataaggacga acctggtgct ggggatcgat ctttcctata gccattccat
     3601 ctcggactat atgaatgccg gtgaaccgaa cgacacccag gcaggcaatt ccttttatgc
     3661 gggtgtcttc ctgcggcgtt acaaacttct gggcaatggt ttttatcttt tcggccaggc
     3721 agaagccggg atcaactacg ggcatacgag tacggattat ccgttgaacg taccgccctc
     3781 gtcggacaaa acgaactcct acgggataca attgttactg tatcccggca tcgcctatgc
     3841 gataaaccaa cattggcaga tagaaacagg gcttccgaat ttctttgccg tgcattacaa
     3901 tcattccagc gagacagccg tagtcacagg ccaatcttcg caggaaagca agagccattc
     3961 tctttatatc agcagctcgc tcggcggcac aaatgcattc accgttggcg ttcgctactt
     4021 catcggcgga taggctttta atatcgggaa taggggagtt agttctacgc gtttaccatc
     4081 cttgttttgc aatcccggcc ttgatcgccg agagcgcttt ttcctcaccc aacagggtag
     4141 tcaacttgtt gcctttcccg tcatgtcctt gcgccatata agcacctttc gcagtcttgg
     4201 tgatgacggc atcctggata ggcacattct tctctttcgt cttaacatta taggccgtga
     4261 gtgttacggt tgacataatt cattgattta aagcaatatt accatttttt acttatacat
     4321 ccaatttcgc atatttcgca tgcgattcga tgaactctct tctcggcgcc acttcgtcgc
     4381 ccatcagcat gctgaaaatg cgatcggcct cggcggcgct ttcgatcgtc acctgcttaa
     4441 gggtgcgcgt attcgggttc atggtcgtat cccacaactg atccgagttc atttcgccca
     4501 agcccttata gcgttgcact gtaacgctat cttcctttcc gccgcccagc ttttctatca
     4561 atccgcgccg ctgatcttca ttccacgcat atgactgttc ttttcccttt ttcacgaggt
     4621 acagcggcgg ttgagcgata tagacataac cctgttcgac catctctttc atatacctgt
     4681 aaatgaacgt gaggatcagg gtggcgatat gactgccgtc cacgtccgca tcggtcatga
     4741 tgatcagctt gtgataacgc agcttggcga ggttcaaagc cttgggatct tccggcgtcc
     4801 cgaccgtcac cccgagtgcg gtgtacatgt tgcggatctc ttcattctca tagatcttgt
     4861 gctccatggc cttttccaca ttgaggatct taccccgcaa cgggaggatg gcctgaaagc
     4921 tacgctctct tccctgtttg gcggtgccgc ctgccgaatc accctcgacg aggaacagct
     4981 cacagcggtc aggattccga tcggagcagt cggccagctt acccggcaaa ccgccgccgc
     5041 tgagcacgct tttgcgttga accagctccc ttgccttttt agccgcggcc cgggcctgtg
     5101 cggcgagtac cacctttgct atgatattct tggcgtctct tggattttct tcgagatagg
     5161 cgtccagcat tctggagacg gtggaatcga ccacaccgct aacctcgctg ttaccaagct
     5221 tggtcttggt ctgtccttcg aactgcggtt ccggcacttt caccgacacg atagtactca
     5281 gaccttcgcg aaaatcatcg ccttcgatct cgaccttggc tctctcgaaa agcccttgtt
     5341 tgtcaccata ggccttgaat acccgggtca gtgcgcggcg gaatccagct acatgtgtgc
     5401 cgccttctat cgtattgatg ttattgacgt aggagtagat gtgttcgcta tagctgtcgt
     5461 tgtaggtcag cgccacctct acggcaacat tggtaacctc gtcccggcct tctacataga
     5521 tgatcttggg tatgaggggt gaccggcccg cattcttgtc gagcatttcc acgaattcca
     5581 cgatgccgcc ttcgctgtaa aaagtcttgc tgtaaggact gccgtcctct tccttttcgc
     5641 gcaaatctag cagggcaatg cgtattcccc ggttgagata agccaattcc cggagccggc
     5701 cttccaggat atccttcttg tacaccgtcg aggtgaaaat actcgtatcg ggccagaaat
     5761 gggtcgtagt gcccgtctta tcgcttttgc cgatctcgcg gacgggatac tgggggacgc
     5821 cgatcttata ttcctgttca aagacctttc cttcgcggaa tacggtgaca tgcagatcgg
     5881 agctgagcgc attgacgcag ctgacgccga cgccgtgcag accgccgctg accttatagg
     5941 tattcttatc gaatttgcct cccgcgtgca aaatggtcat taccagctcg agagcgctct
     6001 tcttttcttt ggtattgata ccggtcggta ttccgcgacc atcgtcttcc accttgatcg
     6061 aattgtcctc gaaaatggtc acctcgatgt tcttgcaata acccgccaga gcttcgtcga
     6121 tggagttgtc caccacctca tataccaggt ggtgcaaacc cttttcgctg atgtctccga
     6181 tatacatggc gggacgtttg cgcaccgcct ccaaaccttc gagaacctgg atactgtccg
     6241 caccgtaacc attgctggcg gccgtcagga catcctgtgt tgtttcgctc attttttaga
     6301 ctgtggaata gagtagttaa caataaatta atagggcctg caaatgtacg aataataagg
     6361 ggtttttttc agttttccgg cgtaaatttg cgggatgttt caggctccgg atatattgac
     6421 gatggcatat aaacaacctg ttatggagga ccagatggtc cttctgctgg agagtcagcg
     6481 gcagataccc gggtcggtac aatacagtat caaacggtac gcaaaacatc cccaatggac
     6541 tgtagaggac ctcgggatga tggtttacca ttacaagcgg gaagacccct ctcagaacta
     6601 cctcgagctt cggttttgtt ttgccggcaa cacgtattgc cggcgatcgg caaattcccg
     6661 gtccggcgga gttgtcatga tcccgggaga ggctgaatgc gactcctgca ggttggaaaa
     6721 cggctccgga tgttccgaaa agatagcgac agtggatgtt ttcaatttca gattcctgcc
     6781 cgtacatctt tcccagtttg tgcggccgcg gaaaccggcg acttccgtca gcgaaaacct
     6841 gcttgaattc aggcataccg cctctttctc caaaatgatg tcgctttgcg gtaggacccg
     6901 cctcgtcctc gagacgctgt taaaccacaa ttataccggc agccatg
//
LOCUS       DASYSV010000216         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_295074, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000216 DASYSV010000000
VERSION     DASYSV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295074"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..301
                     /locus_tag="VGQ51_06665"
     CDS             29..301
                     /locus_tag="VGQ51_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEV9036279.1"
                     /translation="MPTVKFTYALRRYFPTLRDTPTTGTTLTQVLGEMETSYPGVRSY
                     ILDEQGCLRKHVNIFIDGTMINDRRALSDPFEENSEIYIMQALSGG"
     gene            324..1412
                     /locus_tag="VGQ51_06670"
     CDS             324..1412
                     /locus_tag="VGQ51_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEV9036280.1"
                     /translation="MQQTILLGTRKGLIAYHQSNNHWDVENLSFEGQPVSIAYADPRN
                     DVWWACLDHGHWGVKLHRSADRGITWKEVAAPAYPEGEEVNEGVPATTRYIWSMMHGG
                     RSQDHRTTLWIGTDPGGLFRSDDGGDNFRLVESLWNHPTRKTGWFGGGRDLPGIHSII
                     VDPRNEDHIHIGISCAGVFETTDAGKTWEIRNKGLRAEFLPDPESGVGHDPHILVAAP
                     SDPDTLWQQNHCGIFRSKDGANSWVEVSEQEGPARFGFAIAVAEDNPDQAWVAPADSD
                     GNRTAIKGALCICRTDDGGKSWKELRKGLPQKNCFDIVYRHALASSGDALAFGTTTGN
                     LFFSPDRGESWQTLNNYLPMVYSVQFTN"
     gene            complement(1452..2030)
                     /gene="def"
                     /locus_tag="VGQ51_06675"
     CDS             complement(1452..2030)
                     /gene="def"
                     /locus_tag="VGQ51_06675"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV9036281.1"
                     /translation="MILPIVAYGTPILRVVGRDILPDYPDLAKLIEDMWETMYSSNGV
                     GLAAPQVNRDIRLFVMDSKQIFDNLEEDEKDKYPDGPGIRQVFINAHIAELNGEEWAY
                     NEGCLSIPKIREDIFRSEEVTLDYVDENFEPHTSTFNGITARIILHEYDHIEGKLFID
                     YLKPLKRKLLKGKLDDITKGRISVDYKMSFPR"
     gene            complement(2048..2461)
                     /gene="ruvX"
                     /locus_tag="VGQ51_06680"
     CDS             complement(2048..2461)
                     /gene="ruvX"
                     /locus_tag="VGQ51_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV9036282.1"
                     /translation="MARIMAIDYGAKRTGIAVTDPLQIIATGLTTVESSGLIAFLKEY
                     MRTESVELILIGEPKNWDDSDTHATPLVRQVMVRLRKEFPGIPLQPVDERFTSKMASR
                     AMIDMGMKKKQRRNKALVDEIAATILLQEWLERHG"
     gene            complement(2465..2884)
                     /locus_tag="VGQ51_06685"
     CDS             complement(2465..2884)
                     /locus_tag="VGQ51_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV9036283.1"
                     /translation="MAFRLFLDANVLLDFTLKRPEYESARQLIGWAVEGRVEAFITPA
                     IVHITGYWLTRVYKSDLAKKLLLGLLADIQVIDIGHETTINALHSKMIDIEDALQYYT
                     ALHHKLDYFISRDKGLKKAAIPVLPVYSPEEFLKCNQ"
     gene            complement(2874..3116)
                     /locus_tag="VGQ51_06690"
     CDS             complement(2874..3116)
                     /locus_tag="VGQ51_06690"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HEV9036284.1"
                     /translation="MKERLNLTIDGALLEAIKAYAAKRQLSVSELVEGYFKKVTRPAG
                     HKSILEIVDQLPKPAFDPNADLKELFYKEQGEKYGF"
     gene            complement(3162..>3548)
                     /locus_tag="VGQ51_06695"
     CDS             complement(3162..>3548)
                     /locus_tag="VGQ51_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEV9036285.1"
                     /translation="FWVSGFDIIYALQDEEFDRSQQLYSMPSWLGKARALRVSELLHL
                     FSTLFVVAAGVYGGFGWWYWAGVAVFMGMLIYQHAIVKPGDLSRVNLAFMTANGIASV
                     IFAVFVIADLFLSKGPGAWGSHFSRI"
BASE COUNT          882 a         1051 c          805 g          810 t
ORIGIN      
        1 ataccagcca caataacaaa ctttcctgat gccgacggtg aaattcacat atgcgctcag
       61 acgatatttc cctaccctca gggatacccc aacgacgggt acgacactaa cacaggtgct
      121 gggcgaaatg gagaccagct atcccggtgt acgcagctat attctcgatg aacaggggtg
      181 tctgcggaaa catgtcaata tctttataga tgggacgatg atcaacgaca gacgagccct
      241 gagcgatccg ttcgaagaaa acagcgagat ctatatcatg caggccttat ccggaggatg
      301 atcaccattc aaaattaaat catatgcaac agacgatcct gctgggcacc cgaaaaggac
      361 tgattgccta tcaccaatcg aacaatcatt gggacgtgga aaacctttct ttcgaagggc
      421 agcccgtttc tatcgcctat gccgatccgc ggaatgatgt ctggtgggcc tgtctcgatc
      481 acggtcattg gggagtaaaa ttgcatcggt ccgcagaccg ggggatcacc tggaaagaag
      541 tggccgcccc cgcctatccc gagggcgagg aagtgaacga aggcgtaccg gcgacgaccc
      601 gctacatctg gtccatgatg cacgggggcc ggagtcagga tcatcggaca acgctctgga
      661 tcggcaccga tcccggcggc ctttttcgca gcgacgacgg cggcgataac ttccggctcg
      721 tcgagtcctt atggaatcac cctaccagga aaacgggatg gttcggcgga ggacgcgatc
      781 taccaggtat ccactctatt atcgtcgacc cccggaatga agatcatatc cacatcggca
      841 tcagctgtgc cggcgttttc gaaactaccg acgccggaaa aacctgggag atccggaaca
      901 aaggcttgcg agcagagttt cttcccgacc ccgaatcagg cgtaggacac gatccccata
      961 tcctcgtcgc ggcgccgtcc gaccccgata cgctctggca acagaaccac tgtggcatct
     1021 tccggtcgaa ggacggtgcc aacagctggg tagaagtaag cgagcaagag ggtcccgcgc
     1081 gtttcggatt cgctatagca gtggcagaag acaatcccga tcaggcctgg gtcgcgccgg
     1141 ccgacagcga cggcaaccgc accgcgatca aaggcgcgct ctgcatctgc cggaccgatg
     1201 acggtggtaa aagctggaaa gagcttcgga aaggattgcc ccagaagaat tgtttcgaca
     1261 tcgtttatcg ccacgccctc gccagctcgg gggatgcgct ggccttcggc acgaccaccg
     1321 gcaatctgtt cttctccccg gacaggggcg aatcctggca aactctgaat aactatctgc
     1381 cgatggtata ttcggttcag ttcacgaact gaccgcctcg cgccgccatc cgacccgacg
     1441 cgcctcccct ctcaacgcgg gaaactcatc ttataatcta cgctgatcct gccctttgtg
     1501 atgtcgtcca atttaccctt cagcaacttg cgcttcagcg gtttgagata atcgatgaac
     1561 aatttgccct cgatatgatc gtattcgtgt agaatgatac gcgccgtaat gccattgaaa
     1621 gtagaagtat gcggctcgaa attctcgtcg acataatcca gcgtcacctc ctcactgcgg
     1681 aaaatatctt cccggatctt gggtatgctc agacatccct cgttataggc ccattcttct
     1741 ccattcagtt ccgcgatatg cgcattgatg aacacctgcc tgatccccgg cccgtccgga
     1801 tatttgtctt tctcgtcttc ttcgaggtta tcgaatatct gcttggaatc catgacgaaa
     1861 agccggatgt cccggttgac ctgcggagcc gccaatccaa ccccgttgct cgaatacatg
     1921 gtctcccaca tatcttcgat caatttggcc agatccgggt agtccgggag gatgtctctg
     1981 cctaccaccc gcaggatcgg ggttccgtat gccacaattg gtaaaatcat aaatacaaat
     2041 ataaacatta accatgcctt tccaaccact cctgcaacag gatggtggcc gctatctcat
     2101 ccaccaaagc cttgttcctg cgctgttttt tcttcatccc catatcgatc atcgcccgcg
     2161 acgccatctt tgaagtgaac cgttcgtcca ccggctgcaa cggtatcccc ggaaattcct
     2221 tccttaaccg aaccatcacc tgcctcacca gtggcgtcgc atgcgtatcg ctgtcatccc
     2281 agttcttcgg ctccccgatg aggatcagtt ccaccgactc cgtgcgcata tactctttca
     2341 aaaatgcgat cagccccgat gactccaccg tcgtcaatcc cgtcgcaatg atctgcagcg
     2401 ggtccgtaac ggcgatcccc gtccgctttg ccccataatc tatcgccatg atacgcgcca
     2461 tcccttattg attacatttc agaaattctt caggcgaata caccggtaaa acaggaatag
     2521 ccgctttctt caatccttta tccctggaaa tgaaatagtc taatttatga tgcaaggccg
     2581 tataatattg caaagcatct tcgatgtcga tcattttgga atgcaaggca ttgatcgttg
     2641 tctcatgccc gatatcgatc acctgtatgt ccgccagcaa gcccaacaaa agcttttttg
     2701 ccagatcgga tttgtacacc cttgtcagcc aatagcccgt aatgtgcaca attgcggggg
     2761 taatgaatgc ttccaccctg ccctccactg cccacccaat caattgccgg gcactttcgt
     2821 actcaggccg cttgagggta aagtccagca gaacattggc atccaggaat agcctaaaag
     2881 ccatattttt ctccttgttc cttgtaaaac aactctttaa gatctgcatt tggatcaaag
     2941 gccggcttcg gcaattgatc gacgatctct aaaatgcttt tgtggcccgc cggccgcgtc
     3001 acttttttaa agtagccctc aaccagctcg gaaacactga gttgcctctt tgcggcatag
     3061 gccttgatag cctccagcaa cgccccgtca atggttaaat tgagtctttc cttcatcgtc
     3121 caaatatacg cattttttta tattatgcgc attccaaggg cttaaatcct actaaaatgt
     3181 gatccccagg cccccgggcc cttcgacaaa aacagatcgg cgatcacaaa aacggcaaaa
     3241 ataacgctcg caatcccatt cgccgtcata aaggcgagat ttacccggct gagatctccg
     3301 ggctttacaa tagcatgttg ataaataagc attcccataa aaaccgccac acccgcccaa
     3361 taccaccacc caaatccgcc atatacaccc gcagccacga cgaataaggt actgaacaag
     3421 tgcaacaatt ccgaaacccg cagcgcccgc gccttcccca accacgaagg catcgaatac
     3481 aactgctgcg accggtcgaa ttcctcatcc tgtaacgcat aaatgatatc gaaaccgctc
     3541 acccaaaa
//
LOCUS       DASYSV010000217         5102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_677239, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000217 DASYSV010000000
VERSION     DASYSV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677239"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2239)
                     /locus_tag="VGQ51_06700"
     CDS             complement(<1..2239)
                     /locus_tag="VGQ51_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9036286.1"
                     /translation="MRKPLLYLALLLIAATTVSQTLRAQLLPIRNYTTREGLNANSIN
                     AILRDSKGMLWVGTYNGVNWYDGERFLQPQMRTRSGQIYVTQFMEDRRHQVWVATWYS
                     GIYRYAGGAFMNFLPDSEHISDQANSVIDIAELDSGRYLASTDRNAWIFDGRHFTLLD
                     PANPMLNQQIHSILYTRRGDILIGLPQGLAWYRKKGSGWENAGLLLKGIDIDKMAALR
                     DEVWITGSAGLYYIASIGAFLSSSFPQSSYPRTTSSGSAPSPPRSSPRLLLPGPIDNV
                     FPQPNHDCWYTEGKIGACLLHDGRPVRQISKANGLPSTFVKTICTDAEGITWLGTEYG
                     LAKLTPAGYRFFPIREDADNDANIIAIETDATGILWLGTYDGLYRMQGNITREVRKTD
                     GARCGFVFDLLRDHHRRLWACTATGIYTIEKDRVRQWDTRIATAISEDGNGTVWCGCN
                     DGRVLRFDNNSFHEMSQHAIVDERIAGIYHDNAGSLWVGYSLSGIRKFGVSQDSLRLL
                     KEFSENSGNLLVRNMLGDGNGHLLVGTRTGGLYVFDSTGTLWTPVTMDQGLSGNWIKG
                     ISTEAGTIFLATNNGLDILDARRHPFAIRHIPFNDGLVPSEFNTVNLHGDTIWLGTAR
                     GVLEYVPGSQLPDSIPPAIYATRAVINGRSDSSFGHFMHNSRLPRLEYDQNNLSFDFA
                     GLSFRDESAVRYRYRLEGLDNDWSPPTDRRYVNYGNLTPGDYRFLVIASGDDRLWSSQ
                     PASVAF"
     gene            2310..3440
                     /locus_tag="VGQ51_06705"
     CDS             2310..3440
                     /locus_tag="VGQ51_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036287.1"
                     /translation="MKILGSSAILLLFCFGLRAQTRIFKEVGEDISTQMRAITQDNAL
                     VGYLAFTRLEKASEDSFNYRITIMDENLNDIGTVNFRQANLVLQTVSFEANVLCIGYI
                     QTSLTGMQKIRSYNGLQKAEDAASYSHILLQFINLDGKVINTYYKDAHMEVRLAQVSH
                     RAPVVIGAELKYGMQIKNIPKHGFAFFYGDDMMHKLLVFDDKGELRHEIESPMLAERY
                     YLLASGEDIYLMLKQNERVPEGAFTLFDYSARDLRPKNNFDLRDGYDNWLKVLSFDID
                     PASGDAYIAGCIINPKRENRFLTPTDYYSVSNPDTKTNYVIIDDERNIYIYNVNGKKV
                     VRTIPHKDGNVKINVFPAKEGHMMVAEYNKKEKFTRFSIEAL"
     gene            complement(3496..3897)
                     /gene="mce"
                     /locus_tag="VGQ51_06710"
     CDS             complement(3496..3897)
                     /gene="mce"
                     /locus_tag="VGQ51_06710"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEV9036288.1"
                     /translation="MQKVEHIGIAVKDLAASIPLYQTLLNTPCYKTEEVPSERVNTAF
                     FRTGDTKIELLQGLTSDSIITKFVETRGEGMHHIAFEVADIHAEMQRLSAAGFTLLNP
                     TPKPGADNKLVCFLHPKTTGGVLIELCMEKK"
     gene            3923..5083
                     /locus_tag="VGQ51_06715"
     CDS             3923..5083
                     /locus_tag="VGQ51_06715"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEV9036289.1"
                     /translation="MVKLPVYLDYNSTTPCDPRVVEAMLPYFSQKFGNAASRSHSFGW
                     EGEEAVEVAREQVARLIGAETKEIVFTSGATEGDNLALKGVFEAYSAKGNHIITVVTE
                     HKAVLDSCHHIERLGGEVTWLPVDAEGLIDLAELEAAIRPTTVLIAVMYANNEIGVLQ
                     PIRKISAIARRHGVLFFSDATQAVGKVPVDVNADGIDVLTLSAHKLYGPKGIGALYVR
                     RRDPRVRLAAQMDGGGHERGMRSGTLNVPAIVGLGRACAICGEEMEGDALRVALLRDR
                     LEEGLLIEGSRVNGSRLHRLPQTTNISFAGVDGEALLAALGKDIALSSGSACTSASME
                     PSYVLKALGLDDDLAHSSLRLGLGRWTTEEEVDYAIGRIGETVARLRERAYK"
BASE COUNT         1078 a         1159 c         1714 g         1151 t
ORIGIN      
        1 taaatgcgac ggatgccggc tggctgctcc aaagacgatc atcacctgag gcgataacga
       61 ggaaccggta atctcccggc gtgaggttcc cgtaattgac atagcggcgg tcggtgggcg
      121 ggctccagtc attgtcaaga ccctcgagcc gatagcgata acgcacggct gactcatccc
      181 ggaagctgag gccggcaaaa tcgaaggaaa ggttattttg atcgtattcc agtcgcggaa
      241 gacggctgtt gtgcatgaag tgaccgaagc tgctatcgga acggccattg atgacggctc
      301 tcgtggcgta gatggccggg ggtatgctat ccggcagctg gcttcccggg acatattcga
      361 ggacacccct ggcggtgccc agccagatcg tgtcgccgtg cagattgacg gtattgaatt
      421 cggaggggac gaggccgtcg ttgaaaggga tatgccggat ggcaaaggga tggcgtcgtg
      481 catccagtat atcgagaccg ttgttagtgg ccaggaatat cgttccggct tcggtggata
      541 tgcccttgat ccagttgccg ctaagaccct ggtccatcgt gacaggcgtc cataatgtgc
      601 cggtggaatc aaagacgtag agaccgccgg ttcgggtacc caccaacaga tgtccattgc
      661 cgtcgccgag catgttgcgg acgaggagat tgccggagtt ctcggagaat tctttgagga
      721 gccggaggct gtcttgcgac accccgaatt tccggatccc cgaaagcgaa taaccgaccc
      781 agagagaccc tgcattgtcg tggtagatgc ccgcgatccg ttcgtcgaca atggcgtgct
      841 gggacatttc atgaaagctg ttgttgtcga accggaggac ccggccatcg ttgcaaccgc
      901 accagaccgt gccgttgccg tcttcggaaa tggcggtggc gatccgtgtg tcccattgcc
      961 ggaccctgtc cttttcgatg gtatagatgc ctgttgccgt gcaagcccag agacggcggt
     1021 ggtgatcgcg cagcagatcg aagacaaaac cgcagcgggc tccatcggtc tttctcactt
     1081 cgcgggtgat attgccctgc atccggtaga ggccgtcata ggtgccgagc cagaggatgc
     1141 ctgtggcgtc cgtttcgata gcaatgatgt tggcatcgtt gtccgcatct tcccggatgg
     1201 gaaagaagcg atagccagcg ggtgtaagtt tggcgaggcc gtattccgta ccgagccagg
     1261 tgatgccttc ggcgtcggtg cagatcgttt tgacgaatgt ggaaggaaga ccgttggctt
     1321 ttgaaatttg ccggacgggc cggccgtcat gcaacaggca agcgcctatt tttccttcgg
     1381 tgtaccagca gtcgtggttg ggctgaggga agacattgtc gatgggcccg ggcagcagca
     1441 ggcgcggcga ggaacgggga ggggatgggg cggacccgga ggatgtggtc cggggatagg
     1501 aggactgggg aaaggaagaa gagaggaagg cgccgatcga tgcgatataa tatagacctg
     1561 cagaaccggt gatccacact tcgtccctga gggcagccat tttgtcgata tcgatgcctt
     1621 tcagcagcag tcccgcattc tcccatcccg atcctttttt cctgtaccat gccaggccct
     1681 ggggaagtcc tatcaggata tcgccgcgcc gggtgtagag tatggaatgg atctgctggt
     1741 tgagcatagg gttggcaggg tccagcaatg tgaaatgccg tccgtcgaag atccatgcgt
     1801 ttcgatcggt gctggcgaga tagcggccgc tgtccagctc ggcgatatcg atgacgctgt
     1861 tcgcctggtc gctgatatgt tccgaatcgg gcaggaaatt catgaaggct ccgccagcat
     1921 atctgtagat gccgctatac caggtggcga cccatacctg gtgacgccgg tcttccatga
     1981 actgcgtcac gtagatctgt ccgctgcgcg tcctcatctg tggttggaga aagcgttccc
     2041 cgtcgtacca gttgacgccg ttgtaggtgc cgacccaaag catgcctttt gagtcgcgga
     2101 ggatggcgtt aatgctattg gcgttgaggc cttcgcgtgt ggtgtagttg cgaatgggca
     2161 gcagctgggc gcgaagggtt tgcgacacgg tggtcgcggc tatgaggagg agcgccaggt
     2221 atagaagggg ttttctcatg gcagaaagcg gaagacaagg taataaaaat ggataagtgt
     2281 tttcactaat ttttttgaga tattgtggaa tgaaaatact gggatcttcg gcaattcttt
     2341 tgcttttttg cttcggcctc cgcgcgcaga ccaggatctt caaggaggtg ggcgaagata
     2401 tctcgacaca gatgagggcg atcacgcagg acaatgcgct ggtcggttat ctcgcattta
     2461 cgcgtctcga aaaggccagc gaggattcgt tcaactaccg gataacgatc atggatgaaa
     2521 acctgaacga tatcggcacc gtgaattttc gtcaggcgaa cctggtcctt cagaccgtct
     2581 cgttcgaggc gaacgtgtta tgtatagggt atatccagac ttcgctgacg gggatgcaga
     2641 agatcaggtc atacaatggt cttcagaagg cggaggatgc cgcaagttac agtcatatcc
     2701 ttctgcaatt catcaatctc gacggaaaag tgatcaacac gtactataag gatgcgcata
     2761 tggaagtcag gctcgcgcag gtgagccatc gtgcgccggt ggtgatcggg gcggaattga
     2821 aatatgggat gcagataaag aatatcccca aacatggatt tgcatttttc tatggcgatg
     2881 atatgatgca taagttgctc gtattcgacg acaagggcga gcttcggcat gaaatagagt
     2941 cgccgatgct tgccgaacgt tattacctgt tggcctcggg tgaggacatc taccttatgc
     3001 tgaaacagaa cgagcgcgtc ccggaaggcg cttttacgct attcgattat tccgcgaggg
     3061 atctccggcc gaaaaacaat ttcgatctga gggatggata tgacaattgg ctgaaggtac
     3121 tgtctttcga tattgacccg gccagcggcg atgcgtatat agcggggtgt atcatcaacc
     3181 cgaaacgcga gaatcggttt ctcacgccaa cggactatta cagcgtgtcg aaccccgata
     3241 cgaagaccaa ttatgtgatc atcgacgatg agcggaatat ctatatctat aatgtgaatg
     3301 ggaaaaaagt ggtgcggacg ataccacata aggatgggaa tgtgaagatc aatgtgtttc
     3361 cggcgaagga ggggcacatg atggttgcgg agtataataa gaaggagaag tttacgaggt
     3421 tttcgataga ggcgctgtag gggcggttgg ggtatggcgg ttgccgcttc gtggcggtcg
     3481 tggcgggtat ggcggttatt tcttttccat gcaaagctcg atgagaacgc cgccggtggt
     3541 tttggggtgg aggaagcaga ccagtttgtt gtcggcgccg ggtttggggg tggggttgag
     3601 gagggtgaag ccggctgcgg agaggcgttg catttcggcg tggatgtcgg cgacttcgaa
     3661 ggcgatgtgg tgcatgcctt cgccgcgggt ttcgacgaat ttggtgatga tgctgtcgga
     3721 tgtgagtccc tggaggagct cgatcttggt gtcgccggtg cggaagaagg cggtgttgac
     3781 gcgctcgctg gggacctcct cggttttgta gcagggggtg ttgagcaagg tctggtagag
     3841 cgggatgctg gccgcgaggt ctttaacggc gatgccgatg tgttcgactt tttgcataag
     3901 gcaatttagg taactttgag gcatggtcaa actgcccgtc tatctcgact ataacagcac
     3961 tacgccttgc gacccgcggg tagtggaggc catgctgcct tatttttcgc agaaattcgg
     4021 gaatgcggcg agccggagcc atagctttgg ctgggagggt gaggaagcgg tggaggtggc
     4081 gcgggaacag gtggcgaggc tgatcggcgc ggagacgaag gagatcgttt tcacatcggg
     4141 cgccacggag ggcgacaatc tcgcgcttaa gggcgttttc gaggcgtatt cggcaaaggg
     4201 taatcatatc attacggtgg tgacggagca taaggcggtg ctggacagct gtcatcatat
     4261 cgagcggctg ggcggcgagg tgacctggct gccggtggat gcggagggtt tgatcgatct
     4321 cgcggagctg gaggcggcta tacggccgac gaccgtgctt atcgcggtga tgtatgccaa
     4381 taacgagatc ggggtattgc agcccattag gaaaataagc gccatcgcgc gacggcatgg
     4441 ggtgttattt ttcagcgatg ccacgcaggc ggtggggaaa gtgccggtgg atgtgaatgc
     4501 ggacggcatc gatgtgctga cgctgagtgc gcataaatta tatgggccga aggggatcgg
     4561 tgcgttgtat gtacgccggc gcgatccgcg tgtcaggttg gcggcgcaga tggatggggg
     4621 cggtcatgag cgcgggatgc gtagcggcac actgaatgtt ccggccatcg tgggtttggg
     4681 tagggcttgt gcgatctgcg gagaggagat ggagggcgat gcgttgcggg tcgccctcct
     4741 gcgcgatcgg ctggaggagg gtctgctgat tgaagggtcg cgggtcaacg ggagtaggct
     4801 tcaccgtctg ccgcaaacga cgaatatctc tttcgccggt gtagatggag aggcgctgct
     4861 tgcagcgctg gggaaggata tcgcgctttc atcgggttcg gcgtgtacat cggcatccat
     4921 ggagcccagc tatgtgctca aggcattggg tcttgacgac gatctggcgc atagctcgct
     4981 gcgtcttgga ttagggagat ggacgactga ggaagaagtg gactatgcga tcgggcgaat
     5041 tggagagacg gttgcgcggt tgcgggagcg ggcgtacaaa tgaggcacga cgcggctggt
     5101 gc
//
LOCUS       DASYSV010000218         9264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_553887, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000218 DASYSV010000000
VERSION     DASYSV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553887"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VGQ51_06720"
     CDS             <1..957
                     /locus_tag="VGQ51_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036290.1"
                     /translation="FVMPLQVSARSVGRSSESGDAGDEGGEEHRESVYKSILWMKQKY
                     EQYRDMSDQRYEQLKEELVRSEKKYDDLLASIVEVKVGDRREKLLSATAHAVVEERAQ
                     GTTFAVVEERSQGTALAIVEGRAANGQVDGVSEGGGRAGGQVPDEPRPVLANMITAAE
                     IERDSLRDLLAERNQQIIFLQRQLDQRIKDFHLAEHEGRGARGQIQELEEQAARTRQR
                     LEEEAGRIRHQLEDEIARGRRQLEEQTAESKRHLEEQEANAERKLEEKQRVIVDLEEQ
                     ILILRQKIEELVDKLRNNSQLLLNIYQELDKSLRSTDAPAQQ"
     gene            complement(977..2530)
                     /locus_tag="VGQ51_06725"
     CDS             complement(977..2530)
                     /locus_tag="VGQ51_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV9036291.1"
                     /translation="MERHTDSNILIRPVYTAGDLPAGAAQELPGEYPFTRGVQPDMYR
                     GKLWTMRQYAGFSTAEESNARYHYLLSQGVMGLSVAFDLPTQIGFDSDHPLAEGEVGK
                     TGVAIDSLEDMETLFKGIRLEDVSTSMTINATAYILLAFYAALARKQGADWKKITGTI
                     QNDILKEYAARGTYIYPPKPSMRIITDIFEWCSHELPRWNTISISGYHIREAGSTAVQ
                     EIAFTLSNGKAYVRAAMEHGLDIDVFGKRLSFFFNAHNNLFEEVAKFRAARKMWAQIM
                     KTLGATDPKAMAMRFHTQTGGSTLTAQQPLNNISRVTVQTLAAVLGGTQSLHTNGYDE
                     ALSLPTEEAAKIALRTQQIVAYESGAADTADPLAGSYYVEALTKEIEDASWRLIEQID
                     NMGGSVSAIEQGFIQEEIARSAYDYQQRVESKEKIIVGVNSFQGEETMSIPVFRVDDS
                     IRQVQTGKLTDLKKRRDPAKVDSVLQDLNDKAASGENIMPVVVQAVEQRCTLGEIADT
                     LREVFGEYK"
     gene            complement(2530..3069)
                     /locus_tag="VGQ51_06730"
     CDS             complement(2530..3069)
                     /locus_tag="VGQ51_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036292.1"
                     /translation="MIESNYLLIAVIFALLLAGVLVFLGLKQRRELKALQANQTQPPA
                     PATNGGVPTRQLQLQAYERLILLTDRIALPNLIQRVNVPDLGARDMQSLLTQSIRQEF
                     EHNITQQIYVSPEAWDAVRNYKEQNLLIIHQVSSFLPPEATGTDLNKALLDLLVQNPK
                     ASLQNVISDALSYEAKNLM"
     gene            complement(3158..6352)
                     /locus_tag="VGQ51_06735"
     CDS             complement(3158..6352)
                     /locus_tag="VGQ51_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9036293.1"
                     /translation="MNKVFKGILAFSLKNKYFVFFATALLVVYGIITFKDMPIEAFPD
                     VTNTEITIITQWPGRSAEEVEKFVTIPIELAMNPVQKKTSLRSTTIFGLSVVKLIFDD
                     DVEDFYARQQVNNLLPNADLPENVNPQVQPPTGPTGEVFRYTLESSFRDPRELKTLQD
                     WVVDRELRAVPGVADIVSFGGEVKTYEIQVNPMVLNNLGITPLDVFNAVSKSNINIGG
                     DFINKNSQAYVVRGIGLLNDINEIRNIIVENNNGIPVLVKDVANVQISNLPRLGEVAR
                     MDAIDSAHNGPSGGTLSLAGHRRIRTNPDAVEGIILMRKGENPDRVIQGIKNAVNRLN
                     TRILPADTKMVPFYNREDLIKYATHTVLHNLVEGILLVTLIVSLFMFNWRTTLIVSII
                     IPLSLLFAFICLNLMHMSANLLSLGAIDFGIIIDGAVVMVEGLFVVLDRRAHEVGMQR
                     FNHLSKLGMIRRRGGELAKAIFFTKIIIITALLPIFSFQKVEGKLFSPLAYTLGFALL
                     GALIFTLTLVPVLVNLLLRRNVREKHNPIVHFLTKLTMGIFRFNFKHKRLALIVTTVL
                     IGVGLYAFKFLGTEFLPELNEGSIWVRATLPYSISLDTSVAKGEQMQRIMMRFPQVSR
                     IMSQTGRPDDGTDVAGFYNNEFGVALYPQEDWNPKITKEELIDTMSKALSIIPGADLN
                     FSQPIMDNVEEAVSGVKGSICVKIYGDSLDYMEQQSMKVYNVLKHVEGIEDLGVIKNI
                     GQPELDIDLDQSKMALYGVSTADANAVIEMAIGGKAVTQLYEGIRKFDIRIRFPEQYR
                     ATREDIGNLLVPTTSGSKVPVREIARISQKTGPCLIFRDENRRYSAVKFSIRGRDMGS
                     TIAEAQEKVDQVVNLKRGYSMVWQGDFENQQRASKRLAQVVPISLLLIFLLLFVMFGN
                     LKDAGLVFLNVPTAVVGGIVALLVTGTNFSISAGIGFIALFGICILEGVLLITAFKRN
                     LELIRHSQSPLYSSIKLGVNELIRPVLMTALMATIGLLPAAISTGIGSESSRPLARVV
                     IGGLSFATVFSLLIFPLVFFLAYRKVDTLHQQEG"
     gene            complement(6443..7354)
                     /locus_tag="VGQ51_06740"
     CDS             complement(6443..7354)
                     /locus_tag="VGQ51_06740"
                     /inference="COORDINATES: protein motif:HMM:NF021554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HEV9036294.1"
                     /translation="MRFIAILFILLAGIRSDLPAQDFFKASDHYNSGRVAGIIITEAA
                     IGTVVTIGLNYLWYKKFPHSKFHYFNDNGEWLQVDKVGHATTAYNIAAIQSDLFQWAG
                     VPRGTSAIIGTGTALAFMTMIEIMDGHSTKWGFSKGDMLANIGGCLLYQGQQWMWREQ
                     RIGLKYSYHSTIFPKYYPAELGSNLPQRMLKDYNGQSYWLSFNIASFLPASANFPRWL
                     NLSAGYGAEGMIGAFKNPSEVNGSPIPDFKRYRQFYLSFDTDLYRIDGLDPWATTLLK
                     VNRTFKMPAPALEWNEVDGLKFHPLYY"
     gene            complement(7373..8476)
                     /locus_tag="VGQ51_06745"
     CDS             complement(7373..8476)
                     /locus_tag="VGQ51_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9036295.1"
                     /translation="MQKHFNTGHLILLVLMTGGLILHSCETRDKKPVEEKGYVIPDTV
                     MQTLRIDTVAISPLVNAITLTGKVGANEDNVVPVNSLVTGVVQDIKVALGDYVRQGQV
                     LAVVRSQEMAQYSSDLTNAGTNLRIAQKNYDKTVDMYHSGLASATDSFSAGVAVQQAK
                     AELDRINRVLKINGGNDQGEFVVRAPISGFIVQKGATNDMAIRGDNNTSLFTISDLKN
                     VWIQANVYESNISLIHMGENVDVTTLAYPGKVYRGRIDKIMNVLDPTSKVMKVRVVLP
                     NPDYTLKPEMYASITVSDQENKKCLSVPTSAVIFDNSQYYVLVFHSKSDVRITPVKWL
                     NAVGDKTFIAAGVSEGDRVISSQAILIYNALNS"
     gene            complement(8483..>9264)
                     /locus_tag="VGQ51_06750"
     CDS             complement(8483..>9264)
                     /locus_tag="VGQ51_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9036296.1"
                     /translation="SAFASQEGKGYISEKEVVRIQAQLYSFQSEYNDLINQINDTESE
                     LRLILQTRPRSFIDPEVDSNALAALDPDKYPLSTLIDSAYHNRTDLQIARANTKINQL
                     NYNYQKALAVPDLTLSLGYDEAGSFLYNYYGLSASIDLPFFNRNQGNIRNAKTLINST
                     RATEKSTQATVDENVTRSLQKAYDEQRLARTIDPKFFASFDRLMQQVLINYQRRNISI
                     LDFLDFYDSYKQNTLQMNTIRFNLVSAFEDINYYTGTNFYN"
BASE COUNT         1938 a         2264 c         2900 g         2162 t
ORIGIN      
        1 ttcgtcatgc ctctccaagt gtccgcaaga tcggtcgggc ggagcagcga atccggggat
       61 gccggggacg aggggggtga ggaacacagg gaatccgtct ataagagcat cctttggatg
      121 aagcagaagt atgagcaata ccgggatatg tcggaccagc gttacgagca gttgaaggag
      181 gagctggtga ggtccgaaaa gaaatatgac gatctgctgg cgtccatcgt ggaggtaaag
      241 gtcggcgacc gccgggaaaa gctattatcc gcgacggcgc atgcggtcgt ggaggagcgg
      301 gcacagggga cgacgtttgc ggtcgtggag gagaggtcgc aggggacggc gcttgcgatc
      361 gtggaggggc gggcggcaaa cggccaggtg gatggtgtct ccgaaggcgg ggggcgagcc
      421 ggagggcagg tgccggatga accgcgaccg gtcctggcga acatgatcac ggccgccgaa
      481 atagagcgcg actcgttgcg cgatctgttg gccgagcgga atcagcagat catctttttg
      541 cagcggcagc tggatcagcg gataaaggat ttccatctcg ccgagcatga ggggcgcggg
      601 gcgaggggtc agatacagga gctggaggag caggccgccc ggacgcgtca gcggttggaa
      661 gaggaagcgg gtcgtatccg gcatcagttg gaggacgaga tcgcgcgcgg caggcggcag
      721 ctcgaggagc agaccgctga atccaagcgg catttagaag aacaggaggc aaatgccgag
      781 cgaaagctgg aagaaaagca acgggtgatc gtcgacctgg aagaacagat ccttatactt
      841 cgccaaaaaa tagaggaatt ggtagacaag ctgcgcaata atagccagct cttgttgaat
      901 atttaccagg aattggataa atcgttgcgt tcgacggatg cgccggcgca gcagtaagag
      961 gggatgaggg gggcgatcat ttatattctc cgaatacttc gcgaagggtg tcggcgatct
     1021 cgccgagcgt acagcgctgc tccacggcct gcaccacgac cggcatgatg ttctcgccgg
     1081 aggcagcctt atcgttgagg tcctgaagga cggaatcgac ttttgcgggg tctcttcttt
     1141 ttttcagatc ggtcaatttg ccggtctgca cctggcggat ggagtcgtcg acccggaaaa
     1201 ccgggatgga catagtctct tcgccctgga agctgttgac gccgacgatg atcttttctt
     1261 tggattcgac gcgctgctgg taatcgtagg cgctgcgggc gatctcttcc tggatgaagc
     1321 cctgttcgat ggcgctgacg ctgccaccca tattgtcgat ctgttcgata agacgccagg
     1381 aggcgtcttc tatttccttc gtcaatgctt ccacgtagta gctgccggcc aatgggtcgg
     1441 ctgtatcggc ggctccgctt tcataggcta cgatctgctg agtgcgtagg gcgatctttg
     1501 ccgcctcctc ggtaggcagg ctcagggctt cgtcgtagcc attggtgtga agggactgag
     1561 tgccgcccaa tactgcggcc agggtctgga cggtgacgcg gctgatatta ttgaggggtt
     1621 gttgtgcggt aagggtactt ccacccgtct gggtatggaa ccgcattgcc atggccttcg
     1681 gatcggtggc acccagcgtc ttcataattt gtgcccacat cttacgtgcc gcgcggaatt
     1741 tagccacttc ttcgaagaga ttgttatgtg cgttgaagaa aaaggagagg cgtttgccga
     1801 agacatcgat atccagtccg tgttccatag cggcgcgaac gtaggccttg ccgttactaa
     1861 gcgtgaatgc gatctcctgt acggccgtgc tcccggcctc acggatatgg tagcccgaga
     1921 tcgatatcgt attccatcgc ggcagctcgt gactgcacca ttcgaagata tccgtgatga
     1981 tgcgcatcga cggtttggga ggatagatat acgttccgcg ggccgcatat tccttgagga
     2041 tatcgttctg gatggtgccg gtgatctttt tccagtccgc accttgtttc cgtgccaggg
     2101 cggcatagaa ggccagaagg atgtatgccg tggcattgat agtcatggac gtggatacgt
     2161 cttcgagccg gatgcctttg aagagggttt ccatgtcttc cagcgaatcg atcgcgacgc
     2221 ctgtcttccc gacttcgcct tcggcgaggg ggtgatcgct gtcaaagccg atctgggtgg
     2281 gaagatcgaa ggccacgctc agtcccatca cgccctggct gaggaggtag tgatagcggg
     2341 cattgctttc ttcggcggtg gaaaagccgg catattggcg catcgtccaa agtttgccgc
     2401 ggtacatgtc tggctggacg cctctcgtga aggggtattc gccggggagc tcctgcgctg
     2461 cgccggctgg caggtcgcca gcagtgtaca ccggcctgat gagtatattg ctatcggtgt
     2521 gtcgttccat tacatcagat ttttcgcctc gtagctcagc gcgtcggaaa tgacgttttg
     2581 cagtgaggcc ttcgggttct gaacgaggag gtcaagtagg gccttgttga gatcggtgcc
     2641 agtggcttcg gggggcagga aggaactcac ctggtggatg atcagcagat tttgttcctt
     2701 atagttccga acggcatccc aggcttccgg gctgacatag atctgttggg tgatgttgtg
     2761 ctcgaactcc tgccggatgc tttgcgtaag cagggactgc atgtcgcggg cgcccaggtc
     2821 ggggacgttg acgcgctgga tgagattggg aagggcgatg cggtccgtca gcaggatcag
     2881 gcgttcgtag gcctgcagct ggagctgtcg ggtggggacg ccgccattgg tagcgggtgc
     2941 ggggggctgc gtctgattgg cttgcagggc cttcagttcg cggcgttgtt tgagaccgag
     3001 gaagacgagc accccggcga gaagcaatgc aaaaatgacg gcgatcaaaa gatagttcga
     3061 ttcaatcatg cgccaaatgt aagataaatt cagcagcatc gtgggcggcg gcgtgggggc
     3121 gtaaatcaga agccgggtga gcggcggcgt ggggccgtta gccttcctgc tggtgcaagg
     3181 tatcgacctt ccggtaggcc aaaaagaaca ccagcgggaa gatcagcagc gagaataccg
     3241 tcgcaaagct gagaccgccg atgacgacgc gtgccagagg gcgggagctt tccgagccga
     3301 tgcccgtgga gatggctgcg ggtaataggc caatcgtcgc catgagggcc gtcattagca
     3361 ccggccggat gagctcgttg acgcccagct tgatcgagct atacaaggga ctctggctat
     3421 gccggatcag ttccaggttg cgtttgaatg ccgtgatcag taacacgcct tcgaggatac
     3481 aaataccgaa cagcgcaata aaaccgatac cggcggatat actgaaattc gtgccggtga
     3541 ccagcagcgc cacgataccg ccgacgacgg cggtggggac gttgagaaag accagaccgg
     3601 catccttcag attgccgaac atcacgaaca acagcaggaa gatgaggagc aggctgatgg
     3661 ggaccacctg cgcgagccgt ttggacgccc tttgctggtt ttcgaaatcg ccctgccata
     3721 ccatcgaata gcctctttta agattgacga cctggtcgac cttttcctgc gcctccgcga
     3781 tggtgctgcc catatccctg ccgcgaatag agaattttac ggcggaatag cgccggttct
     3841 cgtcgcggaa gatgagacac gggccggtct tctgagaaat gcgggcgatc tcgcggaccg
     3901 gcaccttgga accgctggtc gtgggtacga gcaggttgcc tatatcttcc cgggtagcgc
     3961 ggtattgttc gggaaaccgg atgcggatat cgaatttgcg gataccctcg tacaactggg
     4021 tcacggcctt gccgccgatg gccatctcga tcacagcatt ggcatcggcc gtgctgacgc
     4081 catagagggc catcttactt tgatcgagat cgatatccag ttccggttgt ccgatatttt
     4141 tgatcactcc gaggtcttcg atgccttcca cgtgtttcag gacattgtat actttcatcg
     4201 attgctgttc catgtaatcg agcgagtctc catagatctt tacgcaaatg gaacctttta
     4261 cgccggaaac cgcctcttcg acattatcca tgatgggttg cgagaaattg agatcggctc
     4321 ccgggataat cgacagcgcc ttgctcatcg tatcgatcag ctcttccttg gtgatcttcg
     4381 ggttccagtc ttcctgcggg tagagggcta caccgaattc gttgttatag aaaccggcca
     4441 catcggtgcc gtcgtcgggc cggccggtct gggacatgat acgcgatacc tgcgggaaac
     4501 gcatcatgat gcgctgcatc tgttcgccct tagccacgga agtatcgagt gaaatgctgt
     4561 agggcagagt ggctctgacc cagatggatc cctcgttcag ctcgggcagg aattcggtgc
     4621 cgaggaattt gaaggcatat aatccgacac cgatgagtac cgtggtaacg atcagcgcca
     4681 gccgtttgtg tttgaagttg aaccggaaga tgcccatggt cagcttggta aggaaatgca
     4741 cgatggggtt gtgtttttct cttacgttcc tgcgaagaag gagattgacg agcacgggca
     4801 ccagcgtgag cgtaaagatg agggcgccca ggagtgcgaa gcccagggta taggccagcg
     4861 gggagaacag cttgccttcc actttctgga aggagaagat gggcagcagc gcggtgatga
     4921 tgatgatctt ggtaaagaag atggcctttg ccagctcccc accccggcgg cggatcattc
     4981 ccagcttgga aaggtggttg aagcgttgca tgcccacctc gtgggctctt ctgtcgagca
     5041 ccacgaagag tccttccacc atgaccacgg ccccgtcgat gatgatcccg aagtcgatgg
     5101 cacccagcga gagcaggttg gccgacatgt gcatcaggtt gagacagatg aacgcgaaca
     5161 gcagcgacag tgggatgatg atggagacga tgagggtggt ccgccagttg aacatgaaca
     5221 gcgaaacgat gagcgtcacc agcaggatgc cttcgacgag gttatgcaga acggtatggg
     5281 tggcgtattt gatgaggtct tcgcggttgt agaacgggac cattttcgta tcggcgggaa
     5341 gtatacgggt attcagccgg ttgacggcat tcttgatgcc ctggatcacg cgatcggggt
     5401 tttcaccttt acgcatgagg atgatgcctt ctacggcatc ggggttggta cggatacggc
     5461 gatggccggc cagggacaga gtcccgcccg aagggccatt atgagcgctg tcgatggcgt
     5521 ccatccttgc tacctcgcca agtcttggaa ggttggatat ttgcacattc gccacgtcct
     5581 tgaccagcac ggggatgcca ttgttgttct cgacgatgat attcctgatc tcgttgatat
     5641 cgttcagcag accgatgccg cggacgacat aagcctggga attcttattg atgaaatccc
     5701 ctccgatatt gatattgctt ttgctcaccg cgttgaagac gtccagcggc gtgatgccga
     5761 gattgttcag caccatgggg ttgacctgta tctcgtaggt cttgacctcg cccccgaagc
     5821 tcacgatgtc ggcgactccc ggaaccgcgc gtaattcccg gtcgactacc caatcttgca
     5881 gggtcttgag ctcgcgagga tcgcggaagc tgctttcaag ggtatagcga aacacctcgc
     5941 cggtgggtcc tgtcggcggc tggacctgcg ggttcacgtt ttcggggaga tcggcattgg
     6001 gcaggaggtt gttcacctgc tggcgggcgt agaaatcctc gacatcgtca tcgaagatga
     6061 gtttgacgac cgagaggccg aagatcgtcg tagaacgaag actggtcttt ttctggacag
     6121 ggttcatcgc cagctcgatg ggaatagtaa cgaatttctc cacctcttcg gcgctgcggc
     6181 cgggccattg cgtaatgatg gtgatctccg tattggtgac atcggggaag gcctcgatgg
     6241 gcatgtcctt gaaggtgatg ataccgtata ctacgagcag agccgttgca aagaaaacga
     6301 agtatttatt tttgagcgaa aaggctagga tgcctttaaa gactttattc atgtcggtaa
     6361 ttggtctggc cggctgtgga attggattat cgccgggtaa aactagctat tttcttacta
     6421 ggccggaagt taatttggtt aactaatagt aaaggggatg gaatttcaga ccgtcgactt
     6481 cattccattc cagcgccggc gccggcatct taaacgtccg gttcaccttg agcagggtgg
     6541 tggcccaggg gtccagcccg tcgatacggt agagatcggt atcgaaggag agatagaact
     6601 ggcgatagcg tttgaaatcg ggaatggggc taccgttgac ttccgaagga ttcttaaagg
     6661 cgccgatcat gccttccgcg ccatatcccg ccgacagatt gagccatctt ggaaaattcg
     6721 ccgaggccgg caggaaggag gcgatattga aggacagcca ataggactga ccgttgtagt
     6781 ctttgagcat gcgctgaggc agattgctgc ccagctcggc gggataatat ttcggaaaga
     6841 tcgtggagtg ataggaatat ttgagaccga tgcgttgttc gcgccacatc cattgctggc
     6901 cctggtagag gagacagccg ccgatattgg cgagcatgtc acctttcgaa aacccccact
     6961 ttgtgctgtg gccgtccatg atctcgatca tggtcatgaa ggctagcgcc gtccccgtgc
     7021 cgatgatcgc cgaggttccc ctgggcactc ccgcccattg gaagaggtcg ctctggatcg
     7081 cggcaatatt gtaggcggtg gtggcatgtc cgaccttatc gacctggagc cattcgccgt
     7141 tgtcgttaaa gtaatggaat ttactatgcg ggaatttctt ataccagaga taattgagac
     7201 cgatggtgac gacagtgccg atggcggcct ccgtgatgat gataccggcc acgcggccgg
     7261 agttatagtg gtcggatgcc ttgaagaagt cctgtgcagg aagatcggac cggatgccgg
     7321 ccagcagaat gaacaggata gctatgaagc gcatgcgtaa atataggaac tcttaactat
     7381 tcaacgcatt gtagatcagg attgcctgag aagatatgac gcggtcgcct tcgctgacgc
     7441 cagccgcgat gaaggttttg tcgccgacgg cattcaacca tttgacggga gtgatcctaa
     7501 cgtcggactt gctgtgaaat acgaggacat agtattggct attgtcgaag atcaccgcgg
     7561 aggtcgggac gctgagacat ttcttattct cctgatcgct gacggtgatg ctggcgtaca
     7621 tttcgggttt tagcgtatag tcagggttgg gcagaacgac gcggaccttc atgaccttgc
     7681 tggtcgggtc aaggacgttc atgatcttgt cgatgcgccc cctgtatact ttcccggggt
     7741 aggcgagggt tgtgacatcg acattctcgc ccatgtggat gagactgata ttggactcat
     7801 agacgttggc ctggatccag acatttttca gatcggagat cgtgaacagg ctggtattgt
     7861 tgtcgccgcg gatggccata tcgttggtgg cgcccttttg tacgatgaaa ccgctgatcg
     7921 gcgcgcggac gacgaattct ccctgatcgt tgccgccgtt gatcttcagg acccggttga
     7981 tcctgtccag ttcggccttc gcctgttgta cggctactcc agcgctgaac gaatcggtcg
     8041 cggaggccag gccgctgtga tacatatcga ccgtcttatc gtaattcttt tgggcgatgc
     8101 gaagattggt cccggcgttg gtcaggtcgc tgctgtattg cgccatctcc tggcttctga
     8161 cgacggcgag cacctgtccc tgccgtacat agtccccgag ggccacttta atatcctgga
     8221 cgactccggt gacgagcgaa ttgacgggga cgacattgtc ttcgttggcg cccacctttc
     8281 ccgtgagggt gatggcgttc accagcgggc tgatggcgac agtatcgatc cggagggtct
     8341 gcatcaccgt atcggggatg acatatcctt tttcctcgac tggtttttta tccctggttt
     8401 cgcaggaatg caggatgagg ccgcccgtca tcaggacgag caggatcagg tggccggtat
     8461 tgaaatgctt ttgcataaat aatcagttat agaaatttgt gccggtgtaa tagttgatat
     8521 cctcgaatgc gctgacaaga ttgaaccgga tggtgttcat ctgcagggta ttctgtttat
     8581 aggagtcata gaaatccagg aaatcgagga tgctgatatt ccgtcgctgg tagttgatca
     8641 ggacctgttg catcagcctg tcgaaactgg cgaagaattt cggatcgatg gtacgggcga
     8701 gacgttgttc gtcgtatgct ttttggagtg aacgcgtgac gttctcatcc accgtggcct
     8761 gagtgctttt ttcggtggcc cgggtgctgt tgatcagcgt cttcgcgttc ctgatatttc
     8821 cctggttccg gttgaagaac ggcagatcga tggaagccga gaggccgtag taattgtaca
     8881 ggaagctgcc ggcttcgtcg taaccaagtg acagcgtgag atcggggacg gcgagtgctt
     8941 tttggtaatt atagttcagc tggttgatct tcgtattggc ccttgcgatc tgcagatcgg
     9001 tccggttgtg ataggccgaa tctatgagtg tgctcagggg gtatttgtcc gggtcgagtg
     9061 ctgcgagggc attgctatcg acctccggat cgatgaagga ccggggcctg gtctgcagga
     9121 tgaggcgcag ctcgctttcg gtgtcgttga tctggttgat cagatcgttg tattcgcttt
     9181 gaaaactgta cagctgtgcc tggatgcgaa ccacctcttt ctccgagata tagcccttgc
     9241 cttcctgcga ggcgaaggcg gaag
//
LOCUS       DASYSV010000219        11008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2471223, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000219 DASYSV010000000
VERSION     DASYSV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11008
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471223"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..1560
                     /locus_tag="VGQ51_06755"
     CDS             421..1560
                     /locus_tag="VGQ51_06755"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV9036297.1"
                     /translation="MKLSTKLIHAGVEPDPTTGAIMTPIYQTSTYVQSAPGVHKGYEY
                     ARSQNPTRAALESALAHIENGKHGLCFSSGVAASDAVLRLLVPGDEVIAAGDMYGGTY
                     RLFTKIFEKTGIRFHFVDMQDAEAIRPYLNANTKLVWAETPTNPLMNIVDIAALARLT
                     RQQNILLCVDNTFASPYLQNPLDLGADIVLHSATKYLGGHSDVIHGCLVMNDDQLREK
                     LYFIQKSCGAVPGPQDCFLVLRGLKTLHVRMDRHCENGKVVAHWLRDHPKVAKVYWCG
                     FEDHPGYAVASRQMRGFGGMMSFTLKDDSVESARRVLSSTKIFALAESLGGVESLINH
                     PASMTHASIPRETRISNGLSDSLIRLSVGIEDAEDLTDDLRQAIG"
     gene            1563..2360
                     /locus_tag="VGQ51_06760"
     CDS             1563..2360
                     /locus_tag="VGQ51_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HEV9036298.1"
                     /translation="MDLQAFLDRKVAEYNRPSFIADDPISVPHAFTRRQDIEIAGFFA
                     AMFAWGNRTTIIKKARQLMQLMDDAPYQFVTQHAETDLRRLLDFRHRTFNATDLLYFI
                     AFFRHHYSRYDSLEDAFLLPGGEWNAEAALTAFYPYFFSLDANEGASAEGASVPLAPP
                     RTRKHIATPARRSGCKRINMFLRWMVRRDDNGVDFGLWSRIPVPRLICPVDLHVARVA
                     NRFGLLDRSASDWAAALQLTAQLLNFDSKDPVKYDFALFGLGVMEKF"
     gene            2370..2693
                     /locus_tag="VGQ51_06765"
     CDS             2370..2693
                     /locus_tag="VGQ51_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036299.1"
                     /translation="MDSRELIHDLNRCQGWELTESLTIQQLEGWLAERVNEWIRWDFN
                     ALVSFLYRVDISEAGLRRLLKENPGEDAGLIIAKMVIERLGQKIESRRRYRQNDADTG
                     GEERW"
     gene            complement(2694..3350)
                     /locus_tag="VGQ51_06770"
     CDS             complement(2694..3350)
                     /locus_tag="VGQ51_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEV9036300.1"
                     /translation="MKFEKIHNKGQAKLFRNHYLEMLTKTHPLVIWGIYLPVIVYFLY
                     YGGARLNYSVLTIASVFFGGMVFWSFFEYLMHRFVFHWAAESDRARKFVYVMHGNHHH
                     FPRDKERLFMPPVPSIIMATALFFLFRLALGNAIFLFFPGFMLGYLLYGTMHYAIHAW
                     NPPFKWMKPLWRNHHLHHYKDDHKGFGVSSTLWDLIFGTMFDLKKEKEDKEKVKELMF
                     "
     gene            complement(3459..4718)
                     /locus_tag="VGQ51_06775"
     CDS             complement(3459..4718)
                     /locus_tag="VGQ51_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV9036301.1"
                     /translation="MQRIFICLVAMALTLTAPATAQQRTDPFLDSLLRSQASPLLIRV
                     LDHPDSFHYQLIYTRIDRDAHNRPHFHNEYLHVNALDYFNPASTVKMPLAFLALEKMD
                     SLKRYGVDRDTPMLTDSGFSGQTTAYTDTSSADGLPSLAQYIRKIFLVSDNDAYNRLY
                     EFLGQEAINRRLHQMGYSDIRITRRFVPLSEEENRHTNPLRFVGGDRTLYAQPPAYSH
                     FIFDFSHPVFYGNAYYDWHDSLIHSPMDFTRHNNFPLADAQEMLRSVLFPGSVPRDRR
                     FRLSPGDYRFLYQYMSEYPRESGHPRYDTTEYFDGYTKFFMFHSGRTPIPPYIRVFNK
                     PGWSYGFLTDIAYIVDFKNKVEFMLTGTIYTNSDGILNDDKYDYDEIGYPFFKEVGQI
                     VYRYELARSRRNRPDLREFELTYGPGR"
     gene            4751..5770
                     /locus_tag="VGQ51_06780"
     CDS             4751..5770
                     /locus_tag="VGQ51_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV9036302.1"
                     /translation="MKLLSYLKEGNEQLAILVEDLVYDIELLHPDIPNTMNMFLQYWD
                     ELNPVARAGELAIREGKRNIRSSLISRIEDIQLLAPVPLPPSCRDGYAFRQHVAAARR
                     NRKVPMIPEFDQYPVFYFTNHHGIQGPGDIRCMPDHFEKLDFELEVAILIGRHGRNIT
                     AEEADSHIAGLMIMNDMSARTLQSEEMLLNLGPAKGKDFSTAIGPWLVTLDELQEFGT
                     PCKEGHTGLSWDLRMQCRVNGVPVSDGRLSDMDWTFAELIERASYGADLYPGDVIGSG
                     TVGTGCFLELNGTGKLNDPDYPEQWLLEGDVVELEVDGLGILTNTVVREDSDRSLLAL
                     KKARS"
     gene            5767..6648
                     /locus_tag="VGQ51_06785"
     CDS             5767..6648
                     /locus_tag="VGQ51_06785"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV9036303.1"
                     /translation="MIVDLHTLTPMERQHYLQASVAPRPICFASTIDAAGRVNLSPFS
                     FFNLFSTTPPVLIFAPNRRVRDNTTKHSWQNVLEVPETVINIVDHAMIQQVSLASCDY
                     PKEVNEFIKAGFTEEPATLVRPPMVKESKVKIECRVLETKVLGEQGGSGNLVICEALR
                     MHVDDSLLTEGKRTIDQRRFELVARLGGDWYCRVSPANLFEVEKPNIRLGIGMDALPD
                     AIRISRILTGNQLGQLANVHEMPVVDPAFEDRRLQEIFQYYSVNPEEMEKELHRYAAG
                     LLDEGRVTDAWQVLLAL"
     gene            6713..8218
                     /gene="lysS"
                     /locus_tag="VGQ51_06790"
     CDS             6713..8218
                     /gene="lysS"
                     /locus_tag="VGQ51_06790"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV9036304.1"
                     /translation="MSTSNLSEQEIIRREKLAELSQSGIDAFPAALYPVNSSAAAIKA
                     DYQEGLEVCLAGRLMSRRDMGKASFASLQDSTGKIQVYIKRDEICPGEDKSVYDKVWK
                     HLLDLGDIVGVKGYVFTTKTGEISVHVREFALLTKSLKPLPVVREKEGETFDAVTDPE
                     FRYRQRYADLIVNPQVKEVFVKRTKMINAMRDFLNDRGALEVDTPVLQSIPGGAAARP
                     FITHHNALDVPFYLRIANELYLKRLIVGGFDWVYEFSRNFRNEGMDRTHNPEFTVLEF
                     YVAYKDYLWMMETTEQLLERTAIATNGSTVVEVDGKTIDFKSPYRRVSMYDAIKEYTG
                     EDISGMEENDLRETCHRLRIHVEPSMGKGKLIDAIFSEKCESNFIQPTFIIDYPVEMS
                     PLTKKHRSKPGLVERFELMINGKEMANAYSELNDPIDQRERFEAQLRLMERGDDEAMF
                     IDNDFLRALEYGMPPTAGIGFGIDRLCMLLTGQTSIQDVLLFPQMRPEAQS"
     gene            8331..8711
                     /locus_tag="VGQ51_06795"
     CDS             8331..8711
                     /locus_tag="VGQ51_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036305.1"
                     /translation="MDNATISRIGTIFYALVIAFFGINHFLYGTGMQKMVPAFLPGGV
                     AWVYITGICLLLAAIAFLINRQVRLAGFLLCLFLLLIVLTVHLPAVLNADDANARRFP
                     MTNLIKDTGLAAAALLIAAGAKRT"
     gene            complement(8818..9642)
                     /locus_tag="VGQ51_06800"
     CDS             complement(8818..9642)
                     /locus_tag="VGQ51_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV9036306.1"
                     /translation="MKSFENKSVLITGGTRGIGKAIALRLAAEGANIAIVGKTAEPNP
                     KLEGTIYTAAEEISAAGAGKVLPLQGDIRFDDTIRQVVAATAEAFGGIDILVNNASAI
                     NLTTTEQVEPKRYDLMHDINVRGTFFMSQACIPYLKQAHNPHILNLSPPLNLDSRWFG
                     PHLAYTMSKYGMSMIILGLAEELKQYRIAANALWPRTTIATAAVKNLLGGDFLVQRSR
                     TPEIVADAAFYILQKPSFETTGNFFIDEDVLQQEGITDLGRYAVNPDLKLMNDLFL"
     gene            complement(10223..>11008)
                     /locus_tag="VGQ51_06805"
     CDS             complement(10223..>11008)
                     /locus_tag="VGQ51_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9036307.1"
                     /translation="VVNFRIIKNRSFAIGIVTSFVLGFALYGSVFIFPVFCQNLLGFT
                     AEQTGLILLPGGLATILMMPWVGRMLKNGVPAQLMSAGGFIMFFVFTWMLSNSTLESG
                     TGDFFWPLIVRGLGMSILFVPLTTLALQDLPPQDMGQGTGLNNMMRQLGGSFGIALLT
                     TLIHIKSSSVWANLVGYMNPYNPAFVQRKEQLIQSFRSKGYTMNDAIEAANRALGGTV
                     AKQTMLVTYDQLYLIIGVFVLCCIPIIFLQKFKRRPVIAAGGH"
BASE COUNT         2656 a         2824 c         3103 g         2425 t
ORIGIN      
        1 tgaaggcctg cagcagcttg tcgctgatga tcgcttcacc attcgtagga ttcgcaggat
       61 taacattcgt cgcggcaaag gatcccgtgg tcagggtcag gaaacagcag ccgacaagga
      121 aacttctttt gaccaattgg agcggtgtca tgattcaata atttaagagt agagagaaaa
      181 gattttttgt ttgccggaat aaaactacga ttgtaaaaac gcaagtcaag tgaatctaca
      241 cttccggcaa catcgccggc atgcctgccg cgaagagaaa atgtgaagat gcacccaggg
      301 cggtaaaagc aatcttaaga ctggtgaaac tacacttgaa accgtccttt gcatgttgca
      361 cctaaggggt taagcttttg ttaaactgga tgagttgaat cgatgccgta tctttatgcc
      421 atgaagctct caaccaaact cattcatgcg ggtgtcgagc ccgatcctac gaccggcgcg
      481 atcatgactc ccatctatca gacatcaacg tatgttcaat ctgcccccgg tgttcataaa
      541 ggatatgaat atgcccgctc tcagaaccct acgcgtgcgg cgctggagtc ggcattggcg
      601 catatcgaaa atggcaaaca cgggttgtgc ttcagcagcg gggtagcggc ttcggacgcc
      661 gtgctgcggc tactcgtacc cggcgacgag gtcatcgctg cgggtgacat gtatgggggc
      721 acctaccggc ttttcaccaa aatattcgag aagacgggta tccgcttcca tttcgtcgac
      781 atgcaggacg ccgaagccat ccgcccttac ctcaatgcca atacgaaatt ggtatgggcc
      841 gagacgccta ccaatccact gatgaatatc gtcgacattg cagcactggc gcgtctcacc
      901 aggcagcaga atatcctgct ttgcgtcgac aataccttcg cctccccata tctgcaaaac
      961 ccgctggacc tgggcgcgga tatcgtcctg cattccgcca ccaaatacct gggtggacat
     1021 agtgacgtga tccatggctg cctggtcatg aatgacgatc agcttcgcga gaaattgtat
     1081 tttattcaga agagttgcgg tgccgtaccc ggtccccagg attgttttct tgtgttgagg
     1141 gggctgaaga ccctgcatgt ccgcatggac cggcattgtg agaacggaaa agtcgtggcg
     1201 cactggctac gcgatcatcc gaaggttgca aaggtgtatt ggtgcggatt tgaagaccac
     1261 cccggctatg cggtggcaag cagacagatg cggggatttg gcggcatgat gagctttacg
     1321 ttgaaggacg atagcgtgga atccgcaagg cgggtacttt cttccacgaa aatcttcgcc
     1381 ctggccgaaa gcctgggagg cgtggagtct ctgatcaatc atccggccag catgacacat
     1441 gcctctattc cgcgcgaaac acggatcagc aacggcctga gcgactcact catccgtctc
     1501 agcgtcggta tcgaagatgc ggaagacctg accgacgatc tccgtcaggc aatcggttaa
     1561 ttatggacct gcaagccttc ctcgacagaa aagttgccga atataaccgg ccgtcattca
     1621 ttgccgatga cccgatcagc gtcccgcatg ccttcacacg ccggcaggac atcgagatcg
     1681 ccggtttttt cgcggcaatg ttcgcctggg gcaaccgtac caccatcatc aaaaaagcgc
     1741 ggcagctgat gcagctgatg gatgacgcgc cttaccaatt tgtcacgcag catgccgaaa
     1801 ccgatcttcg ccggctgctc gacttcaggc atcgcacctt caatgccacc gacctgttgt
     1861 acttcattgc cttcttccgg catcattaca gccggtacga ctcactggaa gacgccttcc
     1921 tgttgcccgg cggagaatgg aatgccgaag ccgctcttac ggcgttttac ccatattttt
     1981 ttagtctgga cgctaatgaa ggggcttcgg ccgagggggc ttcggtccct cttgctcctc
     2041 cgcgcacgcg taaacatatc gccacgcctg cgaggcgttc cggctgcaaa cgcatcaata
     2101 tgtttctccg atggatggta cgtcgcgatg acaatggtgt ggatttcggc ttatggtccc
     2161 gcatccccgt cccgcgactc atctgcccgg tggatctcca cgtggcacgc gtagccaacc
     2221 ggtttggcct gctcgaccgg tctgcgtcag actgggctgc ggcattgcaa ctgacggcgc
     2281 aattacttaa cttcgacagc aaagacccgg ttaaatatga ttttgcactt ttcggcctcg
     2341 gcgtgatgga aaaattttaa cgcattcaga tggacagccg cgaactcatt cacgacctca
     2401 atcgctgcca gggatgggag ctcacggaaa gcctcacgat ccaacaattg gaaggatggc
     2461 tggccgaacg ggtgaacgaa tggatccgct gggatttcaa cgcgctggtc tcctttcttt
     2521 accgggtgga tatcagcgaa gccggactga gacgtctcct gaaggaaaac cccggcgagg
     2581 atgcgggcct tatcattgct aaaatggtga tcgaaagatt ggggcaaaag atcgaaagcc
     2641 ggaggcgcta ccggcagaat gatgcggata caggagggga agagcggtgg tgatcagaac
     2701 atcagctcct ttaccttttc cttgtcttcc ttttcctttt tcagatcgaa catagtgccg
     2761 aaaatgaggt cccagagcgt gctgctgacc ccgaagcctt tatgatcgtc cttgtaatga
     2821 tgaaggtggt ggttccgcca aaggggcttc atccatttga aggggggatt ccaggcgtgg
     2881 atcgcatagt gcatggttcc atagagcaga tagcccagca tgaagcccgg aaagaacaga
     2941 aagatcgcat tgcccagcgc gagacggaac agaaagaaaa gggcagtggc catgatgatg
     3001 ctcggaacgg gcggcatgaa cagacgctct ttatcgcgcg gaaaatggtg gtggttgcca
     3061 tgcatcacat aaacgaactt gcgggcgcga tcgctttcgg ccgcccagtg gaagacgaac
     3121 cgatgcatca gatattcgaa aaagctccag aagaccattc ccccgaaaaa gaccgatgcg
     3181 atcgtcaata cggaataatt caaccgcgcc ccgccgtaat agagaaaata aacgataacc
     3241 ggcaggtaga tcccccaaat gaccagggga tgggtctttg tcagcatttc caaatagtgg
     3301 ttcctgaaca acttcgcctg ccctttatta tgtatctttt cgaatttcat ctttccgctg
     3361 tttggttaat ccgccaatat ccggggcctc agacccgcgg aacggcaaaa atacgaagaa
     3421 ccccggaata tcgtccttat ctgccggacc aaaagccctt atctgcccgg accataagtt
     3481 agctcgaatt cccggagatc gggccggttc cggcgggacc tggcaagctc ataacggtag
     3541 acgatctgcc ccacttcctt aaagaaagga tagccaatct catcgtaatc gtatttgtca
     3601 tcgttgagga tcccgtcact attggtatag atggtcccgg tgagcatgaa ttccaccttg
     3661 ttcttaaaat ccacgatata ggcgatgtcg gtgagaaaac cataggacca gcccggtttg
     3721 ttgaaaacac ggatataggg gggtatcggg gtgcggcccg aatggaacat aaagaatttg
     3781 gtatagccgt cgaaatattc ggttgtatca taccggggat gacccgactc ccgggggtat
     3841 tcggacatat attgatatag gaaccggtag tcgcccggac tgagccggaa ccgcctgtcc
     3901 cgcggtacgg aacccgggaa cagcaccgat cggagcatct cctgtgcgtc ggccagcggg
     3961 aaattgttat gcctcgtgaa gtccatcggg gaatggatca gcgagtcatg ccagtcgtaa
     4021 taggcgttgc catagaatac cgggtggctg aagtcgaaga taaaatggct gtaggccggt
     4081 ggctgtgcat aaagggtcct gtccccgccg acgaaccgga gcgggttggt atggcggttc
     4141 tcttcctcac tcagcgggac aaaacggcgg gtaatgcgga tatcggaata ccccatttga
     4201 tggagccgcc ggttgatggc ttcctggcca aggaattcat agagacggtt gtacgcgtcg
     4261 ttatcgctga ccaggaatat cttccggata tattgggcaa gagaggggag accatccgcc
     4321 gagctggtat ccgtataggc cgtcgtctga ccgctaaagc cactgtcggt aagcatcggc
     4381 gtgtccctgt cgacaccata ccgcttcagg ctatccattt tttccaaagc caggaaggcc
     4441 agcggcatct tgaccgtgga ggcgggattg aaataatcca gcgcgttcac atgcaggtac
     4501 tcgttatgga aatgcgggcg gttgtgcgcg tcacggtcga tacgggtata gatcagctgg
     4561 tagtgaaagg aatcgggatg atcgaggaca cggatcagga gcggagaggc ttgcgaccga
     4621 agcaggctgt cgaggaaagg gtcggtccgc tgttgtgcgg ttgcgggtgc ggtaagggtc
     4681 agggccatgg cgacgagaca gatgaaaatg cgttgcatga actaaaggta atgatttacc
     4741 tttggcgacc atgaagcttc tctcctatct gaaagaaggc aacgaacagt tggccattct
     4801 agttgaagat ctcgtctatg atatcgagct gctccacccc gatatcccca acacgatgaa
     4861 tatgttcctg caatattggg acgagctgaa tcccgtcgca cgggccggcg agctggctat
     4921 ccgggagggg aaacggaata tcaggagcag tttgatatca cggatcgaag acatccaact
     4981 attggcgccg gtacccctgc caccttcctg ccgggatggc tatgcctttc ggcaacatgt
     5041 ggccgcagca agacgcaacc ggaaggtgcc catgataccg gaattcgatc aatatcccgt
     5101 tttttatttt accaatcacc atgggatcca gggacccggc gacatcagat gtatgcccga
     5161 tcatttcgaa aaattggatt tcgagctcga ggtggccatc ctcatcggcc ggcatggccg
     5221 caatataacg gcggaagaag cagatagcca tatcgccgga ctcatgatca tgaacgacat
     5281 gagcgcccgg accctccagt cggaggagat gctgctcaac ctcgggccgg ccaaaggcaa
     5341 ggatttctcc acggcgatcg gtccctggct cgttacgctg gatgagctgc aggaattcgg
     5401 gacgccctgt aaggaaggac ataccggtct cagctgggac ctgcggatgc aatgccgcgt
     5461 caatggtgtg cccgtcagcg acggccggct gtccgatatg gactggacct tcgccgagct
     5521 gatcgaacgg gcctcctatg gagccgatct gtatccgggg gatgtcatcg gaagcggcac
     5581 ggttgggacc ggttgtttcc tggagctgaa cggaacgggt aagctgaacg atcccgacta
     5641 tcccgaacaa tggctgctcg aaggcgacgt ggtcgagctg gaggtggacg gactaggcat
     5701 cctgaccaat accgtcgtgc gtgaagacag cgaccgttcc ctcctcgcgc tgaaaaaagc
     5761 caggtcatga tcgtcgatct gcatacgctg acgccgatgg aaagacagca ctacctgcaa
     5821 gcctccgtgg ctccgcgacc gatctgtttc gcaagcacca tcgacgcagc aggccgggta
     5881 aaccttagcc ccttcagttt tttcaatctt ttctcgacca ccccgccggt gctcatcttc
     5941 gcgcccaacc ggagggtgcg tgacaacacc accaaacaca gctggcagaa cgtattagaa
     6001 gtgcccgaaa cggtcatcaa tatcgtcgat catgccatga tccagcaggt aagcctggcc
     6061 agctgcgact atccgaagga ggtgaatgaa tttataaagg ccgggttcac cgaagaaccg
     6121 gcgacgctgg tccggccgcc gatggtaaag gagagtaagg taaagatcga atgccgggta
     6181 ctcgagacca aagttctggg agaacagggc ggttccggca atctcgtgat ttgcgaggcg
     6241 ctgcggatgc atgtagacga ctcgctgctg acggaaggaa agcggacgat cgaccagcgc
     6301 cggttcgaac tggtggccag gcttggcgga gactggtatt gcagggtttc accggctaac
     6361 ctcttcgagg tcgaaaagcc gaatatccgg ctgggcatcg ggatggatgc gttgcctgac
     6421 gcaatccgca ttagccgtat ccttaccggc aatcaattgg ggcagctggc aaatgtccat
     6481 gaaatgccgg tggtcgatcc cgcctttgag gaccggcggt tgcaggaaat attccaatac
     6541 tattccgtga acccggaaga aatggaaaaa gagctgcacc ggtatgctgc cgggctcctc
     6601 gacgagggcc gtgtaacgga tgcctggcag gtgttgctgg cgctgtgata cgcgcccgcc
     6661 tccggcacgc ctttggtcgc tcccgcgaat gacctatctt cgcgggaatt ttatgagcac
     6721 atcgaactta tcggaacagg agatcatccg gcgggaaaag ctggctgagc tgtcgcaatc
     6781 gggcatcgac gcgtttcccg ctgccttata tcccgtcaat agcagcgccg ccgcaataaa
     6841 agcggactac caggaaggac tggaggtctg tctggcgggc aggctgatga gccggaggga
     6901 tatgggaaag gcctcttttg cctcgctgca ggacagtacg ggaaagatcc aggtgtatat
     6961 caagcgggac gagatctgtc cgggagagga caaaagtgtc tatgataagg tctggaaaca
     7021 tctgctcgac ctgggtgata tcgtaggagt gaagggttat gtcttcacga cgaagacagg
     7081 ggagatctcg gtgcatgtcc gggagttcgc attgctgacc aaatcgctga aaccgctgcc
     7141 cgtggtgcgc gaaaaggaag gcgagacttt cgatgccgtc accgatcccg agttccggta
     7201 tcgccagcgt tatgcagatc tgatcgtcaa tccgcaggtg aaagaggtat tcgtaaagcg
     7261 tacgaagatg ataaatgcca tgcgggactt tctcaatgac cggggcgcgc tggaagtgga
     7321 tactccggta ctgcagagca tacccggtgg cgcggcggca agaccgttca tcacacacca
     7381 caatgccctc gacgtgccgt tctatttgcg gatcgcaaac gagctttatc tcaagagatt
     7441 gatcgtaggc gggtttgact gggtgtatga attcagccgc aacttccgca atgaaggtat
     7501 ggatcgtacg cataaccccg agtttaccgt actggaattc tacgtggcat ataaggatta
     7561 tctctggatg atggaaacga ctgagcaatt gctcgagcgg acggctatcg caaccaatgg
     7621 gtcgacggtc gtcgaagtgg atgggaagac gatcgatttc aagtcgccgt accggcgggt
     7681 atccatgtac gacgccataa aagaatatac cggggaagac atcagcggca tggaggaaaa
     7741 tgatctccgc gagacctgtc accggctccg catccatgtt gagcccagca tgggcaaggg
     7801 caaactgatc gatgccatct tcagcgagaa atgcgagtcc aattttatcc agccgacctt
     7861 catcatcgac tacccggtgg aaatgagtcc gctgactaaa aaacaccgca gcaaaccggg
     7921 tcttgtcgaa agattcgagt tgatgatcaa tggaaaggag atggcaaatg cctatagtga
     7981 gctgaatgac cccatcgacc agcgggagcg ttttgaagcg cagctgcggc tgatggagcg
     8041 gggagatgac gaagcgatgt tcatcgacaa tgatttcctg cgcgcgctgg aatacgggat
     8101 gccgccaacg gctgggatag gatttgggat cgaccggctc tgcatgctgc tgacgggtca
     8161 gaccagtata caggacgtcc tcctcttccc tcagatgcgc ccagaggcgc aatcatgagc
     8221 tccattgaaa gacattatgg ctgcgaatag gtgcggccgg agccgcaaac catgaggcgg
     8281 cgtttatgct attttttcgt taaattagaa gtagctaaaa cgaatgtcat atggataatg
     8341 caaccatctc caggatcggg acgattttct atgccctcgt tatcgctttt ttcggtatca
     8401 atcacttctt gtatggtacc ggcatgcaga aaatggttcc cgcttttctc cccggcggtg
     8461 tagcctgggt gtatatcacc ggcatatgcc tgcttctcgc agccatcgcg ttcctgatca
     8521 acagacaggt tcggctggca ggttttctgc tttgtctttt tttattactg atcgtattga
     8581 cggtgcatct tcccgccgtg ctgaatgcgg acgatgcgaa tgccagacgg ttccctatga
     8641 ccaacctgat caaggataca ggtcttgcgg ccgcggccct gcttatagca gcgggtgcta
     8701 aacggacgta acagcaggcg atcttcaaac gaaaacctcc cgctctaagc gggaggacga
     8761 tgttgccttt tacccaagga ttaaaatgaa aaaaaaatta tcgccagacg gctttatcta
     8821 caggaacaga tcattcatga gcttgaggtc gggattgacc gcgtagcggc cgagatcggt
     8881 gatcccttcc tgttgtagaa cgtcctcatc tataaaaaaa ttgcccgtcg tctcaaaaga
     8941 tggtttttgc aggatgtaga aggccgcatc cgcaacgatc tccggagtcc tgctgcgctg
     9001 gaccaggaag tcccctccca gcagattttt taccgcagcc gtagcaatgg tcgtccgggg
     9061 ccaaagggca ttggcagcaa tacgatattg tttcagctct tccgccagtc cgaggatgat
     9121 catactcatc ccgtatttgc tcatcgtata ggccaggtgg gggccaaacc agcggctatc
     9181 cagattgaga ggcggtgaaa ggttcagaat atgcgggttg tgcgcttgtt tcagataagg
     9241 aatgcaagcc tggctcataa agaaggttcc ccggacattg atgtcgtgca tgagatcgta
     9301 gcgtttgggc tcgacctgtt cggtggtcgt caggttgata gcgctggcgt tgttgacgag
     9361 gatgtcgata cctccgaagg cttctgccgt ggcggctacc acctgccgga tggtatcgtc
     9421 gaaccggata tcgccctgca ggggcaggac cttgcccgcg cctgcggcgg agatctcttc
     9481 ggctgcggta tagatcgtcc cttccagctt gggattgggt tcggcggttt tgcctacgat
     9541 ggcgatattg gcgccttccg cggccagtcg caacgcgatg gccttgccga tcccgcgggt
     9601 gccgcctgtg atcagaaccg atttattttc gaaggatttc atcagataaa tactacaaaa
     9661 taggctaaaa aaggaaggtt ccaaagaaat cgggcgaaat gcgggctggc aggggcacgg
     9721 aacgggcctt ccgggggtgg ccgggagtac atgcgggagg gcgaatcgta gatttacgat
     9781 ccggacagta aaatcatcaa ccttgaaatc tagcacttct gctcttgtgc cgggcaaccc
     9841 ggcgttgtat gtttgtattg ttaagtattg atcgattagc gactcaaaaa tccaacgtcc
     9901 gaagaaagcc agaatgaatg aatgaaaccc tgaaatccaa cgtccgaagc aaaaccagta
     9961 ctccaaaatc cttgaaaaag accgctaaga tccactgaaa aacctgaatc ctgattgatc
    10021 aagacttata aagtttaaat ccaaaatcca cgttttatcc aaatcctaag aagaatcgaa
    10081 gttaaaattt gtacctattg atggccaaga aattgatggt taagtatcgt acaaaaacat
    10141 tgagaggtcc cgatagtatc gggacctctc tttttgcatt gactcgtggt ctgccggggc
    10201 ggaatccggg gcggtcagtc gtttagtgcc cacccgcggc tatgaccggt cgccttttga
    10261 atttttgcag gaagatgatc gggatacagc aaagcacaaa tacgccgatg atcaggtata
    10321 gctggtcata agtgaccagc attgtttgtt tggcgacggt gccgccgagg gcgcggttgg
    10381 ccgcctctat ggcgtcattc atggtatagc ccttactcct gaagctctgg ataagctgct
    10441 cttttctttg cacgaatgcc ggattataag ggttcatata gccgacgagg ttagcccata
    10501 cgctggagga cttgatatgg atcagcgttg taagcagcgc gatcccgaat gatccgccga
    10561 gctgccgcat catgttgttc agacccgtcc cctgtcccat atcctgcgga gggagatcct
    10621 gcaatgccag cgtggtgagc gggacgaaca ggatggacat cccgagaccg cgcacgatca
    10681 ggggccagaa gaaatcgccg gtacccgatt cgagggtaga attggacagc atccaggtaa
    10741 atacgaagaa cataatgaat cctcccgccg acatcagctg cgccgggacc ccgtttttca
    10801 gcattcgtcc cacccatggc atcatcagga tcgtcgccag accgccaggt aacaggatca
    10861 ggcctgtctg ttcggcggta aagccaagca ggttctggca gaatacgggg aatatgaata
    10921 ccgagccata cagcgcgaat cccagtacga aggaggtcac gatgccgatg gcgaaacttc
    10981 ggttcttgat gattcggaag ttgaccac
//
LOCUS       DASYSV010000220         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1835358, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000220 DASYSV010000000
VERSION     DASYSV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835358"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VGQ51_06810"
     CDS             complement(<1..1092)
                     /locus_tag="VGQ51_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036308.1"
                     /translation="MKPLSTLILTAALFFVSCHGGPSTIALTTSSMYSIDTVRLADAG
                     GDANAASRKLQEAIDLYRKKGADTARAIELFKSSILLQPTAKAYFELAGALLSTRRFD
                     EGLKALSMAEGLGYSPMANVMFRYAYAYANKPDDTGSLPNSGHVVHYMQLAIQMGYAH
                     PGQFLQKNLFPGAARKSDYDIVFANVLSGGSMRDPSKSLWDAYEGQFPQIGLPLVVDI
                     PWIQQHKLEDVIDFQYEKFIPDMRDAKFSRESSTTFYYIAEVHKEKAFVAVLYGEAYE
                     SAEGDGSELPQYTLVTYDRQGKLIDKMAVAGRTILSDPFLVFSIQPDLKFRVQGFKDV
                     YKDNPDSVGYENNPIIRRDSAPAKYYQIAS"
     gene            1172..>1707
                     /locus_tag="VGQ51_06815"
     CDS             1172..>1707
                     /locus_tag="VGQ51_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV9036309.1"
                     /translation="MIKTDFLVIGSGIAGLSYALKVAQRFPEKKVLVITKTRIDETNT
                     KYAQGGIAGVTDFENDSFDKHIQDTLIAGDGLCNPRTVEIVVTEGPERIQEIIDWGTR
                     FDKDDEGDYKLGREGGHSEFRILHHKDVTGKEIERALLETVGKQKNIEINKHWFVLDL
                     ITQHHLGYLVTKSTPDIE"
BASE COUNT          405 a          438 c          481 g          383 t
ORIGIN      
        1 agacgcgatc tgatagtatt tcgcaggagc ggagtccctg cggatgatgg ggttgttctc
       61 ataaccgacc gagtcggggt tgtccttata gacatctttg aacccctgta cccggaattt
      121 caaatcgggc tgaatgctga ataccaggaa cgggtccgat agtatcgtac ggccggccac
      181 cgccatcttg tcgatcagct ttccctgcct gtcataagtt acgagggtat attgcggtag
      241 ctctgatccg tctccctccg ctgactcata ggcttcacca taaaggacgg cgacaaaggc
      301 cttttcttta tgtacctcgg caatatagta gaaggtggtg ctgctttccc gcgaaaactt
      361 cgcatcccgc atgtcgggga tgaatttttc atattgaaaa tcgatgacgt cttccagctt
      421 atgctgttga atccagggaa tgtcgaccac cagcggcaac ccgatctgcg ggaactgacc
      481 ctcataagcg tcccaaagcg attttgacgg gtcgcgcatc gagcctccgc taagcacgtt
      541 ggcaaacacg atatcgtaat cgctctttcg cgccgctccg gggaataggt ttttttgcag
      601 gaactgaccc ggatgcgcat accccatctg gatcgccagc tgcatatagt gaacgacatg
      661 gccgctgttg ggcaaagagc cggtatcgtc cggtttgttg gcataggcgt aggcataacg
      721 gaacatcacg ttggccatgg gcgaatatcc gagcccttcg gccatggaga gggctttcag
      781 accttcgtcg aaccgacgcg tggaaagcag cgcgccggcg agctcgaaat aggcttttgc
      841 cgtgggttga aggagtatag agcttttgaa cagttcgatg gctcgcgcgg tgtccgcccc
      901 ctttttccga tagagatcga tcgcttcctg caatttccgg ctggcggcgt tggcatcacc
      961 gccggcgtcg gccagtcgca ccgtgtctat actgtacata gaagatgttg tgagggcgat
     1021 tgtcgaagga ccgccgtgac aggagacaaa gaaaagcgcg gcagtgagga tcagggtcga
     1081 taacggtttc atgactaagg gctattgcta cccaagatac cccttttttt taaggttcaa
     1141 aatggcaaac ctgcgctaac ttgcgggtca tatgataaag acagatttcc tcgtcatcgg
     1201 ctccgggatc gccggtctca gctatgcgct gaaggtggcc cagcggtttc cggaaaaaaa
     1261 ggtcctggtc attaccaaga cccgcattga tgagacgaat accaaatacg cgcagggtgg
     1321 gatcgccggc gtaacggatt tcgaaaacga cagttttgac aaacatatcc aggatacgct
     1381 gatagcgggt gacggcctct gcaatccgcg caccgtcgag atcgtcgtga ctgaagggcc
     1441 ggaacgtata caggagatca tcgactgggg cacccgtttt gacaaggacg atgaaggcga
     1501 ctacaagctg ggcagggaag gcggtcattc cgagttccgc atcctgcatc acaaggacgt
     1561 gactgggaaa gagatcgagc gcgcattgct ggagacggtc ggaaagcaaa agaacatcga
     1621 gatcaacaaa cattggttcg tgctcgatct gatcacccag caccatctcg ggtatctggt
     1681 caccaaatcg acgccggata tcgaatg
//
LOCUS       DASYSV010000221        11873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2087170, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000221 DASYSV010000000
VERSION     DASYSV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087170"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VGQ51_06820"
     CDS             <1..253
                     /locus_tag="VGQ51_06820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9036310.1"
                     /translation="FLSVKEALNNVLKHSQSSVVNINVSVNERLVIEIQDDGVGINLD
                     KLRKFGNGLNNMKKRMASIDGEFNIENWKGTRTTFYLQL"
     gene            complement(248..643)
                     /locus_tag="VGQ51_06825"
     CDS             complement(248..643)
                     /locus_tag="VGQ51_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEV9036311.1"
                     /translation="MNRNFLSVAALLGALSVALGAFAAHKLRAMTDPNTVAIFETGVR
                     YQFYHVFALLLVGFLSDKINNRWMIWAGNCFIMGIVFFCGSLYALTALTIAESTHKTL
                     AGIATPIGGVFFIVGWILLYIGVRQKNKS"
     gene            complement(653..1189)
                     /locus_tag="VGQ51_06830"
     CDS             complement(653..1189)
                     /locus_tag="VGQ51_06830"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV9036312.1"
                     /translation="MKIFLIGFMGSGKTHWGRKLSAKLKLPFFDLDSVIVEKEGRSVA
                     AIFTDKGEEYFRFRENELLEALTAEQESFILSCGGGTPCFFNNIEFMKKHGKVVWLNT
                     GVDTLMERLGKEKSSRPLLADVAEGDLRRHIILKLSQRRMYYQQADVVVSEESTSLEE
                     LIRLLLQNQQPDRMREDL"
     gene            complement(1186..2370)
                     /locus_tag="VGQ51_06835"
     CDS             complement(1186..2370)
                     /locus_tag="VGQ51_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV9036313.1"
                     /translation="MAAKTDVFQSPDYFQLDELLTDEHRLVRDAVRSYVKKEISPIIE
                     DYAQKAEFPQQIVAQLGHLGCFGPTIPEEYGGGGLDYISYGLMMQELERGDSGVRSTA
                     SVQGSLVMFPIFEYGSEEQRRKYLPKLASGEWLGCFGLTEPDHGSDPAGMLTNIKDAG
                     DHVILNGSKMWISNAPFSQIAVVWAKDEEGKIRGVIVERGMEGFSTPTTHGKWSLRAS
                     ATGELVFDNVKVPKANILPGVQGLKGPLSCLTKARYGIAWGVVGAAMDCYDTALRYSK
                     ERVQFDRPIGGFQLQQKKLAEMLTEITKAQLLNWRLGVLMSEGRATPAQVSMAKRNGC
                     EIASNIAREARQVLGGMGITGEYPVMRHMMNIESVLTYEGTHDIHLLITGMDITGLNA
                     FK"
     gene            2508..3089
                     /locus_tag="VGQ51_06840"
     CDS             2508..3089
                     /locus_tag="VGQ51_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9036314.1"
                     /translation="MNAVKHAELELTEPILHDELVERCKRGDNRAFAALYQKYSRAMY
                     NTSLRIVNHTGDAEDVLQEAFTDAFRSLDDFHYKSTFGAWLKRIVINKSINQLRKRKM
                     DLIDIEKTNVGVSLSDEEGPDEQEIRMKVEDIRKAVALLPNGYRTVLTLYLFEGYDQE
                     EIAEILQVSHATVRTQYMRAKIKLLQYIKQGAS"
     gene            3086..3796
                     /locus_tag="VGQ51_06845"
     CDS             3086..3796
                     /locus_tag="VGQ51_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036315.1"
                     /translation="MNSRLEQWVRDHREEFDSEEPDNKVWEKISADMSPDKKKRGALV
                     RFNPARWSAAAAVVLVMAGAGWYFSTRSHTSSSQAPVVASSAKSDAGTSGSNSGAKPS
                     PSTQTESENHAGDSQLATIRPAAPAGAAAEIDNSMDEEMYHYARLVEIKHNQLKAIEK
                     DEPLLYRQFASDVSKLDSVYHNLQSQLPKNPNREQLLEAMLQNLQLQMGLLNHQLDII
                     KQINHSKKTEYEKAYKTA"
     gene            3771..4751
                     /locus_tag="VGQ51_06850"
     CDS             3771..4751
                     /locus_tag="VGQ51_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036316.1"
                     /translation="MKRHIRLLSSVTAIIVLSAAMIPTAANAQEAKKQRLINKSYDVT
                     SNDRLIIENQFGNVVVSTWDKPQITVDIEIGARAASDSKAQDIMDKINVRDSRNGREI
                     HFKTDVDEIHNSNKMKGDKGRDNENGRGFYIDYVVHMPASNGLKIDNSFGKTEVPALT
                     GPVTLISKFGSLNAGRLNNVDTVDVEFGKADIDAINNGKVIVKFDKNAHIGKVEGNAK
                     ISCEFSQNVEVRLTNNIQELSLSESYSGVRLIVDKDLSADFEVHSSFGRFHNESDFNI
                     DEKSEEGESGPRFDRDYAGKAGSGKARIRIKSSFGSVRLSHEGGKDRDDG"
     gene            4779..6026
                     /gene="kynU"
                     /locus_tag="VGQ51_06855"
     CDS             4779..6026
                     /gene="kynU"
                     /locus_tag="VGQ51_06855"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEV9036317.1"
                     /translation="MHPTIEPALADAQRRDREDELQSFRSRFYFPTDTPIYFCGNSLG
                     LQPRETETYIRQELADWRQYAIGGYWQARNPWMTYPQSLRPTLCKLAGCQEEEVTVMN
                     ALTVNLHLLLLTFYRPTPHRYKIVMEAGAFPSDQYALETQVKWHGYDPREAIVEVASR
                     QGEFHIREEDIVAAISENGGSLALVLLGGINYYTGQLFDMATITAAAHEAGALAGFDL
                     AHAIGNAPLSLHEWDVDFAVWCSYKYLNGGPGAAGEAFIHERHVRDTGLTRLGGWWGN
                     DESSRFKMGKGFVPKPTAEGWSMSTAQVFNMVALKGSLAIFEEADFARLRAKSHALTA
                     YLDQLLQTLASKKFRVITPERRAAQLSLHFGDRAKALQQSLHRAGVVTDFREPGVIRV
                     SPAPLYNSFEDVYRFYLILRGLL"
     gene            6023..7252
                     /locus_tag="VGQ51_06860"
     CDS             6023..7252
                     /locus_tag="VGQ51_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEV9036318.1"
                     /translation="MKFLSRVSDWLWRRLTILVVIALVAAILLGEFAPATAVRMERLG
                     TAFVSVIKFFIPFIIFFTITTGIAGMSNLRKVGRIGVKALIYFEAVTTLSLAIGIGMA
                     YLVKPGLIDKQGLPIGDASKFIRQGEQGIDWGHFFATNSTLIVLVAAIIAGIVINLVR
                     QRGAILRRLEWASKRVFLALKFVMFLAPVGAFGGMAFTIGKYGLRTLIPLGKLMLTVY
                     ATCIIFVLVVLGLILRYFGMNVLRYLKDIKEEILIVFGTSSSESALPSLMVRLEKMGC
                     SQPVVGLVVPTGYSFNLDGTSIYLSMSVIFIAQLYNVSLSFGELLSILFILMLTSKGA
                     AGVTGSGFTVLAATLTTLHKIPVEGLAFLLAVDKFMSEARAITNIIGNGVATLVIAKS
                     EGEFNPSTTVRPAAVKT"
     gene            complement(7242..7775)
                     /locus_tag="VGQ51_06865"
     CDS             complement(7242..7775)
                     /locus_tag="VGQ51_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036319.1"
                     /translation="MNQCQAPGSASNSYTVKSHSEVHYFVYAGGLPAGDDHQITLDAA
                     VRLINPRIDPKASLNIGLRYSNTGTSTKTTTYDYIYNIEYVYVSKQTHEVFCLPVTFQ
                     YNLLSGRIQPAVYVGFSAAYLSENPADPHILQKFGIAVIGGACIEGYITPHFLIKADW
                     RYELLMQYPSIGVGIKF"
     gene            complement(7779..8312)
                     /locus_tag="VGQ51_06870"
     CDS             complement(7779..8312)
                     /locus_tag="VGQ51_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036320.1"
                     /translation="MKSSSLFLLVALFSVRVFSQTTFSPGYIVKNGDTLRGTLKVDRQ
                     KDLMLRVSFRKDGRTDPTEYTPSDISAFRYDDGALYQTISFVNTAEANRVTETFFGQT
                     LVSGAYSLYRVDKKEATYYIVRKDTSTYFLYDDKISGSDVVNGNYRSLLLLFSSGCEG
                     LDQSRASYTDKSLMGFM"
     gene            complement(8367..9173)
                     /gene="pyrF"
                     /locus_tag="VGQ51_06875"
     CDS             complement(8367..9173)
                     /gene="pyrF"
                     /locus_tag="VGQ51_06875"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV9036321.1"
                     /translation="MTRQQLVSSIRSNGSYLCVGLDTDLSRIPPSLHSAADPIFEFNR
                     RIIDATKDHCVAYKINTAFYEANGARGWETMERTVNHIPSGRLKIADAKRGDIGNTST
                     QYARAFFETLAFDAITVAPYMGEDSVRPFLEYPDKWTIVLGLTSNSGAADFELQPAGQ
                     GLLYERVLTTVAGWGTPENLMYVVGATQASSFTRIRELTPEHFYLVPGVGAQGGSLES
                     ISRHAMNADVGLLVNASRAIIYASGGEDFAEAAEQVAAGYHREMKAFLGF"
     gene            complement(9187..9795)
                     /locus_tag="VGQ51_06880"
     CDS             complement(9187..9795)
                     /locus_tag="VGQ51_06880"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEV9036322.1"
                     /translation="MYIPRRYEEKDKDSIYSFIRENSFAILISVLDGRPVGTHIPLKL
                     ETGEKDALVGHISIGNEQKHTLTEGASVLAIFPGPHAYISPRWYTTMNVPTWNYLSVH
                     VYGTVRLMEGEALREALSRLIDTYESHMPHPVHMQEIPEKMLEDNIRGIVGFRILIDE
                     IQAARKMSQNRDDQSYRQVIDQLDQGDETARAVAAEMKKGKI"
     gene            9990..10547
                     /locus_tag="VGQ51_06885"
     CDS             9990..10547
                     /locus_tag="VGQ51_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036323.1"
                     /translation="MKTFLTALIALFLTLLSCCSRGQGGRQDSTVRKDTGMAAAPVSA
                     SAARQDDEEFNVFLLALGTAFVCVVLGATLAGSMLAILFLLSVFLLVSAGVLSVGILV
                     GFYRRSVAAGFRTVLMIACIGGGVFCGGIAFWLINRIFDIHLRPLTAVLTGAFSGLVG
                     GLLLGLVLFTIIRVFLNYCRKKLAF"
     gene            complement(10589..11734)
                     /locus_tag="VGQ51_06890"
     CDS             complement(10589..11734)
                     /locus_tag="VGQ51_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEV9036324.1"
                     /translation="MTREKVLEALSNVQEPDLGKDIVTLNMVKDLEIEGNYVAFTVVL
                     TTPACPMKDHIKNACVNAVRLLVDKDATVNVRFTANTTTHRKDAGEVLPKVKNIIAVV
                     SGKGGVGKSTVAANFALALARGGASVGLMDADIYGPSVPIMFGVRGQRPMMMPGGAEG
                     KGMIVPLDRYGIRLMSIGLLVDEKNAVVWRGPMASSAIRQFVTDVHWDELDYLVIDMP
                     PGTGDIHLTLLQTVPVTGVIVVTTPQDVALADAKKGIAMFSQAQLKAPIIGLVENMSY
                     FTPAELPDHKYYIFGKEGGKRLAEEYDIPFLGQIPLVQSIREGGDAGIPIMMSDDAVS
                     KKAFLDFAGNAARSIAMLNANIRAEKVTERIEGYEQGRARDEALTKF"
BASE COUNT         2767 a         3288 c         3067 g         2751 t
ORIGIN      
        1 cttcctatcc gtcaaggagg ccctgaacaa tgtgctgaaa cattctcaaa gctcggtcgt
       61 caatatcaat gtctcggtga acgagcggct ggtcatcgag atccaggatg acggcgtcgg
      121 catcaatctc gacaagctgc ggaaattcgg taacggcctg aataatatga agaaaagaat
      181 ggcttcgatc gacggggagt tcaatatcga gaactggaaa gggacgcgca ctaccttcta
      241 tctgcaatta tgacttgttt ttttgccgca ctccgatata cagtaatatc caaccgacaa
      301 tgaaaaatac gcccccgatg ggagtggcga tcccggccag cgtcttatga gtactttcgg
      361 caatcgtcaa tgcggtgagg gcatacaacg atccgcagaa aaaaacgatc cccatgatga
      421 agcaattgcc cgcccatatc atccaccggt tgttgatctt atcggagaga aatccgacca
      481 gcagcaatgc gaagacatga tagaactgat aacggacgcc cgtttcgaaa atggccacgg
      541 tattgggatc ggtcatcgcc ctcagcttgt gcgccgcaaa cgctcccagc gcaacggaca
      601 atgcgcccag aagggctgcc acggatagaa agtttcggtt catagtatta ttttacaagt
      661 cctctctcat tcgatcgggt tgctggttct gcagcaacag gcggatcaat tcctccaggc
      721 tggtgctctc ctcgctcacc acgacgtccg cctgctgata atacatccgc cgctggctca
      781 gtttgaggat gatatgacgc cgcagatcgc cttccgctac atccgccagc aagggcctgc
      841 tgctcttttc tttccccagc cgctccatca gcgtatccac gccagtattc agccagacga
      901 ccttcccatg ctttttcatg aattcgatat tgttgaaaaa acagggagtc ccgccaccgc
      961 aggacaggat aaagctttcc tgctcagccg tcaaagcttc cagcagctcg ttctcacgga
     1021 aacggaaata ttcttcgcct ttatcggtaa aaattgcagc gaccgaacgc ccctccttct
     1081 cgacgatcac cgaatccagg tcgaagaaag gaagttttaa cttcgccgat aatttgcggc
     1141 cccagtgggt cttgccggaa cccatgaatc cgatgagaaa aattttcatt tgaatgcgtt
     1201 tagacccgta atgtccatac cggtgatcag caggtggata tcgtgggtcc cctcataggt
     1261 cagaacgctt tcgatattca tcatatgccg catgaccggg tattccccgg taatacccat
     1321 gccccccaac acctggcgag cctcgcgcgc gatgttggag gcgatctcgc agccgttgcg
     1381 tttggccatc gacacctggg cgggggtggc cctgccttcg ctcatcagga ccccgaggcg
     1441 ccagttaagt aattgagcct tagtgatttc cgtcagcatt tcggccagtt ttttctgttg
     1501 cagctggaac ccgccgatag gacggtcgaa ttgcacgcgc tctttactat atcgcaaggc
     1561 cgtatcataa caatccatcg cggccccaac gacgccccag gcaatcccat aacgcgcctt
     1621 tgtaagacag ctcagcggcc ccttcaatcc ctgcactccg ggaaggatat tcgctttggg
     1681 gaccttcaca ttgtcgaaga ccagctcgcc cgtcgccgag gcgcgcagac tccattttcc
     1741 gtgggtggtc ggcgtcgaaa aaccttccat acccctttcg acgatgacac cccgtatctt
     1801 cccttcttcg tcctttgccc agaccacggc tatttgggag aaaggcgcat tgctgatcca
     1861 cattttactc ccgttgagga tcacatgatc gcccgcatcc ttgatatttg tcaacattcc
     1921 tgcaggatcg ctgccatgat cgggttcggt gagaccaaaa cagcccagcc attcgccgct
     1981 ggccagcttc ggcaggtatt tccgtcgctg ctcttcgctt ccatattcga atatggggaa
     2041 catcaccaat gagccctgga ccgaagcggt actgcgtacg ccgctatcac cccgctccag
     2101 ttcctgcatc atcagaccat agctgatata atccaacccg ccaccgccat actcctcggg
     2161 gatggtaggg ccgaaacatc ccagatgccc caactgggca acgatctgct gcgggaattc
     2221 tgctttttgg gcatagtcct cgatgatcgg ggatatttcc ttttttacat agctgcgcac
     2281 ggcgtcgcgc accagtctat gctcatcggt caacaattca tccagctgaa aataatccgg
     2341 gctctgaaaa acatccgtct ttgcggccat gggaaacagt tttgtatttt tatgcggcct
     2401 ccggggggcc gcgctgatgc gaatgtaaga aaaatggcca ttttttggcc acccgtgcaa
     2461 catcggaaaa agggccgtgt acttaatagt acatgaacgc caggtaaatg aacgctgtaa
     2521 aacatgccga attggaattg accgaaccaa tactacacga cgagctcgta gaacgctgca
     2581 aacggggcga caaccgcgct tttgcagccc tctatcagaa atattccagg gcgatgtata
     2641 ataccagtct ccggatagtc aatcataccg gtgatgcgga ggatgtatta caggaggcat
     2701 ttaccgacgc gttcaggtcg ctggatgatt ttcactataa atccacgttc ggagcgtggc
     2761 tcaaaaggat cgtgatcaat aaatccatca accaattgcg caaacggaag atggacctga
     2821 tcgatatcga aaagaccaac gtcggggtaa gcctgtccga tgaggaaggc cccgatgaac
     2881 aggagatccg gatgaaggtg gaggatatca ggaaggcggt cgccttattg cccaacggct
     2941 accgaacggt cctgaccctg tatctcttcg aagggtatga tcaggaggag attgcagaga
     3001 tcctgcaggt atcgcatgca acggtgagga cgcaatacat gcgcgcaaaa attaaattgt
     3061 tgcaatatat aaaacaagga gcatcatgaa cagtaggtta gaacagtggg tccgtgatca
     3121 ccgcgaggaa ttcgactccg aagagccgga caataaggtc tgggaaaaga tcagtgcgga
     3181 catgagtccc gacaaaaaaa aacggggggc gctggtccgg ttcaacccgg cccggtggag
     3241 cgccgcggcg gccgtcgtcc tcgtaatggc gggagcgggc tggtatttta gcacccggtc
     3301 gcataccagc tcttcacaag ctcccgtggt agcctcctcc gcgaagtctg acgccggaac
     3361 ttcaggttcc aattcaggcg caaagcccag cccgtcgact cagaccgaaa gcgaaaacca
     3421 cgccggcgat tcacagctgg cgacgatcag acccgcggca ccggccggcg ctgccgccga
     3481 gatcgataat tcgatggacg aggaaatgta tcactacgcc cggctggtcg aaatcaagca
     3541 caaccaattg aaagccatag aaaaagacga acctcttctc tacaggcagt tcgcatcgga
     3601 cgtcagcaag ctggatagtg tctatcacaa cctccagagc caactaccga aaaaccctaa
     3661 cagggagcaa ttgcttgagg cgatgttaca gaatcttcag ctgcagatgg gattactgaa
     3721 tcatcagttg gatattatca aacaaatcaa tcattcaaaa aagacagaat atgaaaaggc
     3781 atataagact gcttagttcc gttaccgcca tcattgtctt atctgctgcg atgatcccca
     3841 cggccgccaa cgcacaggaa gcaaagaagc agcgactgat caataaatcc tacgacgtga
     3901 cctcgaacga caggctgatc atagagaacc agttcggcaa tgtggtggtc tccacctggg
     3961 ataaaccgca gatcaccgta gacatcgaga tcggggcgcg ggccgcctcg gacagcaagg
     4021 cgcaggacat catggacaag ataaatgtac gcgactcccg caacggacgt gaaatacact
     4081 tcaagaccga tgtggacgag atccacaact cgaataaaat gaaaggcgat aagggtcgcg
     4141 ataacgaaaa tggccggggt ttctatatcg attatgtcgt gcacatgccg gcctccaacg
     4201 gactgaagat cgacaacagt tttggtaaga ccgaagtgcc ggccctaacc ggacccgtca
     4261 ccctgatcag caaattcggc agcctcaacg ccggccggct gaacaatgtg gacacagtag
     4321 atgtggaatt cggcaaggcg gacatcgatg cgatcaataa tgggaaggtc atcgtcaaat
     4381 tcgacaagaa tgcgcacatc gggaaagtag aaggcaacgc gaagatcagc tgtgagttct
     4441 cgcagaatgt ggaggtgcgt ctgaccaaca atatccagga gttgtcgctt tccgaatcct
     4501 attccggcgt acgtctcatt gtcgataagg atctgtcggc cgatttcgag gtccactcaa
     4561 gcttcggccg atttcacaac gagagcgatt tcaacatcga cgaaaaaagc gaagaaggcg
     4621 aatcgggacc gcgtttcgac cgcgactatg caggtaaggc cgggtccgga aaggccagga
     4681 tcaggataaa aagcagtttt ggctcggtgc ggctctcgca tgaaggtggt aaagaccggg
     4741 atgacggcta aaccgtatct tgcatcaaat tctttttgat gcacccgaca atcgagccgg
     4801 cgctggccga tgcgcaacgc agggaccggg aggatgagct gcaaagcttt cgctcccggt
     4861 tttattttcc taccgataca cccatttact tctgcggcaa ctctctcgga cttcaaccgc
     4921 gcgaaaccga aacctatatc cggcaagagc tcgctgactg gcgccaatac gccataggcg
     4981 gctactggca ggcgcgtaac ccctggatga cctatcccca aagcctgcgg cccaccttat
     5041 gcaaactggc aggctgccag gaggaggagg tcaccgtcat gaatgctctc acggtcaatc
     5101 tccatctcct ccttctgacc ttctaccgcc ccactccgca tcgctataag atagtcatgg
     5161 aggccggcgc gttcccgtcg gaccagtatg ccctggaaac gcaggtgaaa tggcatggtt
     5221 atgacccgcg tgaagccatc gtcgaggtgg cgtcccggca gggtgaattt catatccgtg
     5281 aagaagatat cgtggccgcc attagtgaaa acggtggatc gctggcgctg gtgctcttgg
     5341 gcggcatcaa ctattataca ggtcagctgt tcgatatggc gacgatcacc gctgccgcgc
     5401 acgaagccgg cgcactggcg ggtttcgatc tggcgcatgc catcggcaat gcaccgctga
     5461 gcctgcatga atgggatgtc gatttcgccg tctggtgttc ttataaatat ttgaacggcg
     5521 gcccgggggc cgccggagaa gcgttcattc atgaacgaca cgttcgtgat acgggcctca
     5581 cccggctggg cgggtggtgg ggaaatgacg aatccagcag gtttaagatg ggaaaagggt
     5641 tcgttcccaa acccaccgca gaaggctgga gcatgagtac ggcgcaggtc ttcaatatgg
     5701 ttgcgctgaa agggtcgctg gctattttcg aggaagcaga tttcgcccgg cttcgcgcca
     5761 aaagccacgc cctgacggct tatctcgacc agttgctcca aaccctcgca agcaaaaaat
     5821 ttcgggtgat cacccccgaa agacgggcgg cgcagttgtc gcttcatttc ggcgatcgcg
     5881 cgaaagccct tcagcaaagc ctgcaccggg ccggggtcgt caccgatttt cgcgagccgg
     5941 gagtcatccg cgtcagcccc gctccgctat acaactcctt cgaagacgtg taccggtttt
     6001 atcttatctt acggggacta ctatgaagtt cctctcacgc gtgtcggact ggttatggcg
     6061 gcgtctgacc atactcgtgg tcatcgccct tgtcgcggca atcctattgg gcgagttcgc
     6121 tccggcgacg gccgtccgta tggaacgtct tggcaccgcg ttcgtctccg tcatcaagtt
     6181 cttcatcccc tttatcattt tctttactat caccaccggc attgccggca tgagcaatct
     6241 caggaaggta ggtcgcatcg gcgtaaaggc actgatctat ttcgaggccg tgaccactct
     6301 ctcactagcc atcggcattg gtatggccta tctggtgaag ccgggtctca tcgacaaaca
     6361 gggcttaccg ataggagacg cctccaaatt catacggcag ggagaacagg gcatcgactg
     6421 gggtcacttt ttcgccacta acagcacgct catcgtcctg gtcgctgcta tcatcgcggg
     6481 catcgtgatc aatctcgtaa gacaacgcgg cgccatcctc cgccggttgg aatgggcttc
     6541 gaagcgcgtt ttcctggcgc taaaattcgt catgttcctc gccccggtag gcgcttttgg
     6601 cggtatggcc tttaccatcg gcaaatatgg cttgcgcacc cttatccccc tcggaaagct
     6661 gatgctgacg gtctatgcga cctgcatcat ttttgtgctg gtcgtgctcg gcctgatcct
     6721 ccggtatttc gggatgaatg ttttgaggta tctgaaggat atcaaagaag aaatcctgat
     6781 cgtcttcggc acctcctctt cggaatcggc gcttccctcg ctgatggtcc ggctggagaa
     6841 gatgggatgc agtcagccgg tggtaggact cgtcgtaccg acgggttata gcttcaatct
     6901 cgacggcacc tccatctatc tttcgatgtc ggtgatcttt attgcgcagt tgtataatgt
     6961 gtcgctaagc ttcggcgagt tgttgagcat cctgtttatt ctaatgctaa cgtccaaagg
     7021 cgccgcggga gtcacaggca gcggttttac ggtattggcc gccaccctca ccacgctcca
     7081 caagataccg gtcgaagggc ttgccttcct gcttgccgtc gacaaattca tgagtgaggc
     7141 acgtgcgatc accaatatca taggcaatgg agtcgctacg ctggtcatcg cgaaaagtga
     7201 gggggagttc aacccttcca ccacagtgcg gcccgccgcg gtcaaaactt aatccctaca
     7261 ccgatggaag gatattgcat cagcaattca tatcgccagt ctgccttgat gagaaaatgc
     7321 ggagtaatat agccttcgat acacgcgccg ccgatgactg caatcccgaa tttttgtagg
     7381 atgtgggggt cggcggggtt ttccgacagg taggctgcag aaaagccgac atagactgcc
     7441 ggttggatcc tgccggagag taagttatat tgaaaagtga ccggcaggca gaatacttcg
     7501 tgggtttgtt tactcacata tacatactcg atgttataaa tgtaatcgta tgttgtggtt
     7561 ttggtgctgg tgcccgtatt cgaatagcgc aacccgatat tcagggaagc ctttggatcg
     7621 attctcggat ttatcaatcg cactgccgca tcgagcgtta tctggtgatc gtcgccggcg
     7681 ggcaatccgc ccgcatatac gaaatagtga acctcggaat gagattttac agtataactg
     7741 ttactggcac tacccggggc ctgacattga ttcacccgtt acataaaacc catcaggctt
     7801 ttatccgtat atgacgctct ggattggtcc aggccttcgc aacctgagga aaacaatagc
     7861 agcagacttc ggtaattgcc attgacgaca tcggaaccgg agatcttgtc atcatatagg
     7921 aagtaggtgg aggtgtcctt cctgacgata taataggtgg cttccttctt atccacgcgg
     7981 tacaaggaat aggcgccaga gacgagggtc tgcccaaaaa aggtctcagt caccctgttc
     8041 gcttcggctg tgttcacgaa ggaaatggtc tggtacagcg caccgtcatc ataacgaaag
     8101 gcggagatat ccgacggggt gtattctgta ggatccgtcc ggccgtcttt cctgaaactt
     8161 acccggagca tcaggtcctt ttggcggtcg accttcaatg tcccgcgaag agtgtccccg
     8221 ttcttgacga tatatccagg cgagaaggtt gtttgggaaa aaacgcggac tgaaaacagg
     8281 gccaccaata gaaataggga ggacgatttc atacgtaagc cgttagaggt tatagtatgg
     8341 ctcaatataa ggaatcaggc tgaactttaa aaccccagga aggctttcat ttctctatga
     8401 taacccgcgg cgacctgttc agcagcctcc gcgaagtcct ctccccccga tgcatagatg
     8461 atcgcacggc tggcattcac cagcaatccg acatcggcat tcatggcatg ccgcgagata
     8521 gattcgaggc tgccgccctg cgctccgaca ccgggcacga gataaaaatg ttcgggtgtc
     8581 agctcacgga tacgtgtaaa tgacgaagct tgcgtcgcgc ctaccacgta catgaggttt
     8641 tcaggggtgc cccatcccgc gacagtggtc agcacacgct cgtataacag tccctgcccc
     8701 gcgggttgaa gctcgaaatc agccgcgccg gagttggagg tcagcccgag cacgatagtc
     8761 catttgtccg ggtattccaa aaaaggccgg acgctgtcct cacccatata gggagccacg
     8821 gtaatggcgt cgaaggccag cgtttcgaaa aatgcgcgcg cgtattgcgt ggatgtattt
     8881 ccgatatccc cgcgtttcgc atccgctatt ttcaaacggc cggaagggat atgattgacg
     8941 gtcctctcca tcgtctccca accccgggcg ccattcgcct catagaaggc cgtgttgatc
     9001 ttataggcca cacaatggtc tttggtcgcg tctatgatcc ggcgattgaa ttcgaaaatg
     9061 gggtcggcgg cggaatgcag cgatgggggg atccgtgaaa ggtccgtgtc gagaccgacg
     9121 caaagatatg acccgttgga gcgaatggaa gagacgagtt gctgtcgcgt catagggcaa
     9181 agatattcaa attttccctt ttttcatctc ggcagctacg gccctggcgg tctcatcacc
     9241 ctgatcaagc tgatcgatca cctgtcgata gctttggtcg tcgcggttct ggctcatctt
     9301 tcgcgcggcc tggatctcat cgatgaggat acgaaaaccg acgatgccac ggatattgtc
     9361 ctccagcatc ttttcgggta tctcctgcat atgcaccggg tgcggcatat ggctctcgta
     9421 ggtgtcgatc aaccgggaca acgcttccct aagcgcctcg ccttccatca gccgtaccgt
     9481 accatatacg tgaaccgaaa gataattcca ggtggggaca ttcatcgtcg tataccagcg
     9541 cggggaaata taggcgtgtg ggcccgggaa aatggccagc accgatgcgc cctccgtaag
     9601 cgtatgcttt tgttcattgc caatggagat atgccccacc agcgcatctt tctccccggt
     9661 ctccagtttc aacgggatat gcgttccgac cggacggccg tccagtacgc tgataagaat
     9721 ggcgaacgag ttctcgcgga tgaacgaata gatcgagtcc ttgtcttttt cttcgtagcg
     9781 gcggggaatg tacatcccgc aagatagcga ttattcgcaa cgcccttccc gcacgtcccc
     9841 ggcaatgcac tccttcccgc acgtccccgg caatgcactc cttcccgaac gcccccggca
     9901 aagcactcct tcccgcctgc tcagcttttg atgggtggca acgttttgcc cctaaaatgc
     9961 gtacttttag gtactccata attgatctga tgaaaacgtt tctaacggct cttatcgccc
    10021 ttttcctcac actgctgtct tgttgcagcc ggggacaggg agggcggcag gattctacgg
    10081 tcagaaagga caccggcatg gctgctgctc cggtttccgc ttccgcggcc cggcaggacg
    10141 acgaagaatt caatgttttt ctcctggccc ttggcacagc cttcgtctgt gttgtcctcg
    10201 gcgcaactct tgccggatcg atgctggcaa tccttttctt attgagtgta ttcctccttg
    10261 tctcggcagg cgtgctttcg gtagggatcc tggtcggatt ctatcgccga tcggtggcgg
    10321 cgggattcag gacggtactg atgatcgcat gcatcggcgg cggggtattt tgcggcggca
    10381 tcgctttctg gcttatcaac cggatattcg atattcatct gcgacctttg acggcagtac
    10441 tcaccggagc cttcagcggg ttggttggag ggctgcttct cgggctcgtc ctcttcacga
    10501 tcatccgggt tttcctcaat tattgcagga aaaaattggc gttctgattg acgccgcgtc
    10561 gcgaggcctc gagccctacg ccaccacctc agaacttcgt cagagcttcg tccctcgctc
    10621 ttccttgttc gtaaccttcg atcctctcgg ttactttctc cgcgcgaata ttcgcattca
    10681 gcatggcaat actccgcgcg gcattccccg caaaatccag aaaagccttt ttactcaccg
    10741 catcatcgct catcatgatc ggaattccgg catcgccccc ttcgcggata ctctgcacca
    10801 gcggtatctg acccagaaaa gggatatcgt attcttccgc caatcgcttg ccaccctctt
    10861 ttccaaaaat gtagtattta tgatcgggca attcggcagg cgtgaaatag ctcatattct
    10921 ccaccagtcc gatgatgggg gccttcagtt gagcctgact gaacatagcg atcccttttt
    10981 tagcgtcggc cagggccaca tcctggggcg tcgtcactac gatcactccc gtaaccggga
    11041 cggtctgcaa caaggtgaga tggatatctc ccgtgcccgg aggcatgtcg atcaccaggt
    11101 aatccaactc gtcccagtgc acatccgtga caaactgcct gatggcgctg ctcgccatag
    11161 gacccctcca caccaccgca ttcttttcgt ccaccaacaa tccgatgctc atcagcctga
    11221 tgccatagcg atccaaagga acgatcatcc cttttccctc ggcgccgccg ggcatcatca
    11281 tcggccgctg accacgtacc ccgaacatga tcggaacgct tggaccatag atgtctgcat
    11341 ccatcagacc tacgctggcg cctccacggg ccagggccag ggcgaaattg gctgcgaccg
    11401 tactcttccc caccccgcct ttgccgctga cgacggcaat gatattcttt actttgggta
    11461 atacttcgcc ggcatcttta cgatgggtgg tggtattggc agtgaatcgt acgttcaccg
    11521 tagcatcctt atcgaccaat agtctgaccg cattaacgca ggcgttcttt atatgatcct
    11581 tcatcggaca cgcgggcgtg gtcaacacga cggtaaaggc cacataattg ccttctattt
    11641 ccagatcctt taccatattc agcgtgacaa tatcctttcc cagatcgggt tcctgaacat
    11701 tgctgagagc ctctaaaact ttttcgcgag tcatgcgcaa tattttgtta aaaactttaa
    11761 tttgagacaa aattaacccc tgtcggcctt actttgttta agaatttgcg gtttaaaagt
    11821 ttaattttga gaccgaatga aaagattttt actatacttt ctgctactat ccg
//
LOCUS       DASYSV010000222         3725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1447259, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000222 DASYSV010000000
VERSION     DASYSV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447259"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VGQ51_06895"
     CDS             complement(<1..827)
                     /locus_tag="VGQ51_06895"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEV9036325.1"
                     /translation="MAFIDELLFTPSYGWKDDTGALVIPTSKQLWNEAFSRINVFRTK
                     KNWISFVSFLMLAGMLPFFYLFLFKYFSWYLVIAIVIYSMVIMGTHGTIWFHRYCTHK
                     SFGFSHPLWRILTQNLVIKTVPEEIYVVSHHVHHSKSDEPGDPYNAQAGFWYCMLAEY
                     NHQRVSPDLSEPAHKKAVHFMEHTGVRLNSYRQYQKWGSIACPFYTVAIWVLNWTFWY
                     FVLSWIGGPGLACALFSAAMLWFALIRAFNYTGHGGGKEKHREGVDFDRSNLSINQS"
     gene            complement(954..3470)
                     /gene="ppsA"
                     /locus_tag="VGQ51_06900"
     CDS             complement(954..3470)
                     /gene="ppsA"
                     /locus_tag="VGQ51_06900"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEV9036326.1"
                     /translation="MNPSVIDLRDIDRTKLSVAGGKAANLGELFRVEGISVPGGFIIS
                     TEAFKRVVSETPSLTGSLDRLSHLKTNDRNGVGKISGDIRRLIEGITIPADITEAIAT
                     RLETRGEKQPYAIRSSATAEDLPTASFAGLQDTYLNITGKDAILTHITKCWASLFTER
                     AISYRLHNGFDYRKVQIAVIVQQMVLAQAAGVLFTADPITSNRTILSIDAGFGLGEDL
                     VSGLVDPDNYQVRNGAITDKRICSREQVLSDEQILRLGQTGRKIERHFGHPQDIEWCL
                     AADNTFYIVQSRPITTLFPVPATKDAKNHVYASVGHQQMMTDAMKPLGISFWQLTAAR
                     PMFTAGGRLSVDIAENLASPVSRKMLIDVLGKSDPLIKDALLTLTKDDFVPSGQDGPN
                     TPMRDFETLQEYDPAIVHDLIKTSQGSIDQLKQNIRSKSGGELFDFILDDICQLKKFL
                     SDPRSFGVIMTAMNASSWVNGKMQEWLGEKNAADTVSQSAANNITSEMGLALLDVADV
                     IRPHPEVIHFLTAVKDEDLSDRLPEFNGGLRSWDAIRAFLDKFGMRCVGEIDITRTRW
                     SEKPSILVPMILSNIKNFGPNAGHRKFGQGRQEALKKEQELLDRLALLPEGKQKAEQT
                     KKMIGLIRTFIGYREYPKYAIVSRYFIYKQALKAEAEVLVKDGILSEKEDIFYLSFEE
                     LHEVVRTRRLDGTIIGQRRNEHQLNEKLNPPRIITSDGEIVTGRYNRDDLPAGAFAGL
                     AVSSGVVEGRARVILNMEDADLQKGDILVTRFTDPSWTPVFVSIKGLITEVGGLMTHG
                     AVIACEYGLPAVVGVADATKLIKDGQKIRVNGTEGYIEIL"
     gene            3532..>3725
                     /locus_tag="VGQ51_06905"
     CDS             3532..>3725
                     /locus_tag="VGQ51_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036327.1"
                     /translation="MKNLIGKILTNIPYIRHAYVSKHNSHFRPGHYYSPVPDVDELEE
                     RQAEIWRPKTLAGIDLNEEE"
BASE COUNT          835 a          913 c         1026 g          951 t
ORIGIN      
        1 cttgactgat tgatggaaag attgctacga tcgaagtcga ccccttcgcg atgtttttct
       61 tttcccccgc catgtccggt ataattgaat gccctgatca gcgcaaacca aagcatggca
      121 gcggagaaaa gagcgcaggc cagtccggga ccaccgatcc atgaaagcac aaaataccag
      181 aaggtccagt tgagcaccca gatcgcaacc gtataaaagg ggcaggcgat cgatccccat
      241 ttctgatatt ggcgatacga attcagcctt acgccggtat gctccatgaa atgcaccgcc
      301 tttttatgag ccggttcgga tagatcgggc gatacccgct gatggttgta ttcggccagc
      361 atgcagtacc agaatcctgc ctgcgcgtta taaggatcgc cgggctcgtc cgatttggag
      421 tgatgcacat gatgcgaaac gacataaatc tcctccggta cggtttttat tacgagattt
      481 tgcgtcagga tccgccacag tggatggcta aagccaaagg acttgtgcgt gcaatagcgg
      541 tgaaaccaga tggtgccatg cgtacccatg atcaccatgc tataaatgac aatggcgatg
      601 accaggtacc aggagaaata tttgaagagg aagagataaa agaagggcag cattcctgcc
      661 agcatcagga agctgacgaa agagatccag tttttctttg tccggaagac attgatccgc
      721 gaaaaggcct cgttccaaag ctgttttgag gtaggaataa ccagggcgcc ggtgtcgtcc
      781 ttccatccgt aggaaggggt gaagaggagc tcgtcgataa aggccataac agataatttc
      841 ggattttaat gaaaacgcct taataacgaa agcgtcgatg tttgttgtat accgggcaca
      901 agatatcgaa ataaagagag ttgtgaataa ttatgtgtga tcgcgggccc gatctatagg
      961 atctctatat aaccctccgt gccatttacc cggatctttt gcccgtcttt gatcaatttg
     1021 gtagcgtcgg ctactccgac gactgccgga aggccgtatt cacatgcaat caccgctcca
     1081 tgggtcatga gccctccgac ctcggtgatc aggcccttta tcgatacgaa caccggtgtc
     1141 cagctgggat cggtaaaccg ggtgaccaat atatctccct tttgcagatc ggcatcttcc
     1201 atgttcagga tcacgcgcgc tcttccttca accacaccgg atgaaacggc cagacctgca
     1261 aaagcgccgg cggggaggtc atcccggttg tatctgccag taacgatctc gccatcggag
     1321 gtgatgatgc gcggcgggtt cagtttctcg tttagttgat gttcgttcct gcgttggccg
     1381 atgatcgttc catccagccg gcgggttcgt acgacttcgt gaagctcttc aaaagagaga
     1441 tagaagatat cttccttttc ggaaaggatc ccgtctttta cgaggacttc ggcttcggct
     1501 tttaaggcct gtttgtagat gaaatagcgg ctgactatgg catacttcgg atattcgcga
     1561 tacccgatga aggtccggat cagcccgatc atttttttcg tttgttcggc tttttgtttg
     1621 ccttccggca gtagtgccag ccggtcgagc agctcctgct cttttttcaa ggcttcctgt
     1681 cgtccctgcc cgaatttccg atggccggca ttcggcccga agtttttgat attgctcagg
     1741 atcatgggaa cgaggatgga tggtttttcg ctccagcggg tcctcgtgat atcgatctcg
     1801 ccgacgcagc gcattccaaa tttgtcgaga aaagcccgga tagcgtccca ggatcgcagt
     1861 ccgccgttga attcagggag tcgatcggag aggtcttcgt cttttaccgc cgttaaaaaa
     1921 tggatgactt cgggatgggg ccggatcaca tctgctacat ccaaaagcgc cagacccatt
     1981 tccgacgtga tattgttggc tgcagattgg gaaactgtgt cggccgcgtt cttttctccc
     2041 aaccactcct gcattttccc gttgacccag gaagaggcgt tcattgcggt catgatcacg
     2101 ccaaaacttc gggggtccga taggaatttt tttaattggc agatatcgtc gaggataaaa
     2161 tcgaaaagtt ccccgccgga tttcgatcgg atgttctgtt ttagctggtc gatcgagcct
     2221 tgactggttt tgatcagatc gtgaacgatg gcaggatcgt attcttgcag cgtctcgaaa
     2281 tcccgcattg gcgtattggg tccgtcttgt ccggacggga cgaaatcgtc tttcgtaagg
     2341 gtcagtagtg cgtcttttat cagcggatcg gatttcccga ggacatcgat cagcattttt
     2401 cgggagaccg gtgaagccag gttttccgcg atgtccacgg atagccgccc gccggcggtg
     2461 aacatgggcc gggccgcagt caactgccag aaagagattc ccaggggttt catggcgtcg
     2521 gtcatcattt gctggtgacc gacggaggcg tagacatggt ttttcgcatc ctttgtcgcg
     2581 gggaccggga ataaggtcgt gatcggccgg ctttggacga tataaaatgt gttatcagca
     2641 gccaggcacc attcgatgtc ctggggatgg ccgaaatgtc gttcgatctt tctgcctgtt
     2701 tgtccgaggc gcaggatctg ttcatcggac agcacctgct cccttgagca tatcctttta
     2761 tcggtgatgg cgccattccg cacctggtaa ttgtcaggat ccaccaggcc ggagacgagg
     2821 tcctcgccga ggccgaagcc tgcatcgatg gagaggatcg tcctattgga cgtgataggg
     2881 tcggcggtaa atagaacgcc tgctgcctgc gccaggacca tctgctgaac aatgacggct
     2941 atttggacct tacggtagtc gaagccgttg tgtaaccggt aggatatggc tctttcggta
     3001 aacaacgatg cccagcattt ggtgatatgc gtcaggatgg cgtcttttcc ggtaatgttc
     3061 aggtaggtgt cctgcaggcc tgcaaaagag gctgtcggca ggtcttcggc cgtggcgctg
     3121 gatcgtatgg catagggttg cttttctccg cgtgtttcga ggcgggtggc gatcgcttcc
     3181 gttatgtccg ccgggatggt gatgccctcg atgagccggc ggatatcgcc gctaatttta
     3241 ccgacgccgt ttcgatcgtt tgtcttcaga tgcgatagcc ggtccagtga tccggtgagc
     3301 gacggggttt cgcttacgac ccttttgaag gcttcggtcg aaatgatgaa accaccgggg
     3361 acgcttattc cttcgaccct gaaaagctca cccaggtttg cggccttacc ccccgcgacg
     3421 gatagtttgg tcctgtcgat atcgcgaagg tcaattacag atggattcat cgttttatat
     3481 tatcttgggg ggcttaaatg agaggaaatt cagaatatca gcctaatgat catgaaaaat
     3541 cttatcggta aaatacttac aaatataccc tatatacgtc atgcatatgt ctccaagcat
     3601 aattcgcact ttcggcccgg ccactattat agcccggtgc ctgatgtgga tgaactggag
     3661 gaaagacagg cggagatctg gcgaccgaag actttggcgg ggatcgatct gaatgaggag
     3721 gagca
//
LOCUS       DASYSV010000223         5510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_422926, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000223 DASYSV010000000
VERSION     DASYSV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422926"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VGQ51_06910"
     CDS             complement(<1..1180)
                     /locus_tag="VGQ51_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV9036328.1"
                     /translation="MTAEQFSEIAGTIPLSPGIYKYFGDDDDLIYVGKAKSLRKRVSS
                     YFNKTVASYKTYELVQRIRRIEFTIVGSEQDAFLLENSLIKQYQPRFNINLKDDKSYP
                     YIVIKNEPFPRVFLTRRKINDGSEYLGPFTSVGRVREMLDLVKSNIQLRTCQLNLAES
                     QVRKGKYKVCLEYHLGNCKGPCEGLQTGEDYAAGLVQLKNILRGQLGPLIQEFKKEMR
                     ELAEKMDFEKAEMVRKKIEHLENYDSRSVIVSRHLSHADVFSIARDGDLAYINYLMVE
                     NGTIVQTHTTPVETHLEETDEEVLSFAAAQLRETFNSRAPELILPFAIEFPEEGIVQT
                     IPKGGDKKKLLDLSEKNVHYFREELRRKKILQLEGKTADQRRQVLNQLMEDLQLSELP
                     V"
     gene            complement(1189..1788)
                     /locus_tag="VGQ51_06915"
     CDS             complement(1189..1788)
                     /locus_tag="VGQ51_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036329.1"
                     /translation="MKYLAFLLLAGAAASFAACDHSNPPDVNTGKDSVSNPYFPVADY
                     LESEILYVDSTPTALWKYVTHKGRTDSSFIGVPEFNSLALQFLPPAIRDSGWEHHFTE
                     TSFSDKTTRSITFSYSPRDEGQGVQRVDVLTTPGVRTQEVRTIYVEQSRKSGDSLILQ
                     KMLWRSKIGFQIVTLTRVKGQDLDEQQIKVVWGSEREDE"
     gene            complement(1813..3456)
                     /locus_tag="VGQ51_06920"
     CDS             complement(1813..3456)
                     /locus_tag="VGQ51_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV9036330.1"
                     /translation="MKESNFYSDDFEQLIRDKTEQYKMYPSENVWKGVHNSLHTKRKW
                     FIGSMSVLVTGILFMAGRELIAPSVHPIAARKTAPAAGSMGDLSKSSESSGQAGTLHT
                     PLAAYRPVTAALNSRHTAGADNATEELDPGYKGLNITIGNPVLSQSDISEWLSHVVRL
                     PEHAPDLAVVESKSAMAEQWRAAEDGSHPQDIARTSAEVVAGHREVVGETGTKDESDD
                     VRNVLESLSASAASGRVSHPGARVLQNPGSERSSVTGKNADVANERGGASAKADAAAI
                     SEADDMQRINWLRDYAMNILPPSSKRGRTFLQLTLSPTLNYRTLNGVDPQMEKFGTTN
                     TGSLNPSPGLGFEVGGSVLYRLTRNLSIKAGLQFNFIRYQIGTYATNNWQTQGMAYLT
                     PYGYFVDSMRQHSTRQANQNNTMPPKTVVTLDNDYYQLSAPIGFELRVLGNEKLQLHV
                     GATVQPSYLLSTTAYMLSEDNTSYDKLPSMFRKWNVSGGVEAFLTYRMGNIRWQIGPE
                     FRYQLLSSYTSKYPNGDQYPITENLKSYGIKFGIVKPLP"
     gene            complement(3497..4072)
                     /locus_tag="VGQ51_06925"
     CDS             complement(3497..4072)
                     /locus_tag="VGQ51_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9036331.1"
                     /translation="MTEEAILQGCLKNEAAAQRELYNRYSPKMLAVCYRFAHNREDAE
                     DMLQEGFIKVFSQMHTFQNKGAFEGWIRRIIVHTCINNLKKNKRFNESLDIVHAHGVQ
                     VREETVPSIVQAKQIVECIRVLPIGYRTVLNLYAIEGYSHKEIADMLDIEESTSRSQY
                     TRAKQMLEEILIRKKILTKPREKTEWLMAVR"
     gene            complement(4199..>5510)
                     /gene="gpmI"
                     /locus_tag="VGQ51_06930"
     CDS             complement(4199..>5510)
                     /gene="gpmI"
                     /locus_tag="VGQ51_06930"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEV9036332.1"
                     /translation="GRIVNQELQRINLAIRGGSFFQNPALLSVIRGAKKEGKALHLLG
                     LVSDGGVHSHIRHLKAIVDACLKEGLTRVFIHAFTDGRDTDPKSGLGYIRDLQEHLNI
                     TTGKIATVSGRYYAMDRDKRWERVKLAYDALVKGAGEKATDALAAIENSYAAGITDEF
                     IKPTVIAGSDQLPLATIQDGDYALCFNFRTDRCREITEVLTQQDFPEQGMKKLALHYT
                     TMTQYDARFKGVGVMFENDDLKNTLGEVIAEHGLKQIRIAETEKYPHVSFFFSGGRET
                     PFEGERRIMVPSPKVATYDLKPEMSAVELTDAIVPEIRDRSADFICLNYANADMVGHT
                     GVWGAAIKAAETVDRCVERVVSAALDSGYIVFLTADHGNADYMINGDGTPNTAHTMNP
                     VPFFVIDKGQKGGIRSGKLGDLAPTILTMMGLEIPAEMTGEILI"
BASE COUNT         1154 a         1526 c         1440 g         1390 t
ORIGIN      
        1 gcaccggcag ctcggaaagc tgcaggtcct ccatcagctg attcaatacc tggcggcgct
       61 gatcggccgt ctttccctcc agctgcaaga tcttcttacg tcgaagctcc tcacggaaat
      121 aatggacatt cttttcggaa aggtccaaca gcttcttctt gtcacccccc ttcggtatcg
      181 tctgtacaat gccttcttcg ggaaattcga tggcgaaagg caggatgagc tcgggcgccc
      241 ggctgttaaa ggtttcccgc agctgggctg cagcgaagga gaggacctct tcatcggtct
      301 cctccaggtg agtttcgaca ggcgttgtat gggtctggac gatcgtgccg ttctccacca
      361 tgagatagtt gatataagcc agatcgccgt cacgtgcgat cgaaaagaca tcggcatgac
      421 tcagatgacg gctgacaatg acagaccggg aatcgtagtt ctcgaggtgt tcgatcttct
      481 tgcggaccat ctcggccttt tcgaaatcca tcttttcggc cagctcgcgc atttcctttt
      541 tgaactcctg tatcagcgga cccagctggc ccctgaggat attcttcagc tgcaccagac
      601 ccgctgcata gtcctccccg gtctgcaacc cttcacaggg acccttgcaa ttgccaagat
      661 gatactcaag acaaacttta tacttcccct tccggacttg agactcggcc aggttcagct
      721 gacaagtcct taactggata ttactcttca ccaaatcgag catctcgcgt acccggccca
      781 ccgatgtaaa cggacccaga tattcggacc cgtcattgat ctttcgccgg gtaaggaaca
      841 cccggggaaa aggttcgttc ttgatgacga tatagggata actcttgtcg tctttcaggt
      901 tgatgttgaa tcttggctgg tattgtttga tcagcgaatt ttccagcaaa aaggcatcct
      961 gctccgaacc cacgatcgtg aattcgatac ggcggatccg ctgcaccagc tcataggtct
     1021 tgtagctcgc gaccgtcttg ttgaaatacg aacttaccct tttccgaagg ctcttggcct
     1081 tgccgacata gatgagatca tcgtcgtctc caaaatactt gtaaataccc gggctaaggg
     1141 gtatcgtacc ggctatctcg ctgaattgtt cggctgtcat gatatctatt attcatcctc
     1201 ccgttcgctg ccccagacta cttttatctg ctgttcatcc aggtcctgcc ctttcacgcg
     1261 ggtaagggtc acgatctgga agccgatctt cgatctccac aacatttttt ggaggatcag
     1321 gctgtcgccg gatttgcggc tttgctcgac ataaatggtc cgtacttcct gcgtacgtac
     1381 acctggtgtg gtcagcacgt ccacgcgttg cacgccctga ccctcgtctc gcggcgaata
     1441 gctgaacgtg atcgatctgg tcgtcttgtc cgagaaagag gtttcggtga aatgatgctc
     1501 ccatccgctg tccctgatcg ccggcggcag gaattgaaga gccagcgaat tgaattccgg
     1561 caccccgatg aagctggagt ccgtcctgcc cttatgcgtt acatatttcc agagggcggt
     1621 cggcgtcgaa tcgacataca ggatttcgct ttccaggtaa tcggccaccg gaaaataagg
     1681 gttggagaca gaatcctttc cggtattgac gtcaggcggg ttggaatgat cgcaggcggc
     1741 gaacgatgcc gcggcgcccg ccaacaaaag gaacgcgaga tatttcatga agtaaaggta
     1801 agaagacttt actcagggca gtggtttcac gatcccgaac ttgattccgt agctcttcag
     1861 gttctcggtg atgggatact gatccccgtt ggggtatttg gaagtatagg aagaaagcag
     1921 ctgataacgg aactcgggtc cgatctgcca gcggatatta cccatgcgat aggtaaggaa
     1981 ggcttccact ccgccgctga cgttccattt tctgaacatc gagggcaatt tgtcatagga
     2041 agtattgtct tccgacagca tataggcggt agtgctgagc agatagctgg gctgtacggt
     2101 ggcccccaca tgcaactgaa gcttctcgtt gccgagaacg cgcagttcga agcctatcgg
     2161 ggcggacagc tggtaataat cgttatccag cgtcacgacc gtcttcggag gcatcgtatt
     2221 gttttggttg gcctggcgcg tagagtgctg ccgcatggag tctacgaaat aaccataagg
     2281 agtaagatag gccatcccct gcgtctgcca gttgttggtc gcataggtgc cgatctggta
     2341 ccggataaaa ttgaattgca gtccggcttt tatcgagaga ttgcgtgtca gcctgtaaag
     2401 tacgcttcca ccgacttcga aacccagacc cggcgatgga ttcagcgaac cggtgttggt
     2461 cgtgccgaat ttttccatct gtgggtccac gccgttcagt gtcctgtagt tcagggtggg
     2521 ggataacgtc agctggagaa aggtccttcc gcgtttggac gacggcggta ggatattcat
     2581 ggcatagtca cggagccagt tgatgcgttg catatcatcc gcctccgaaa tggctgcggc
     2641 atcggccttt gcggaagccc cccccctttc attagcgaca tccgcgttct tacccgttac
     2701 cgagctacgt tccgaaccgg gattttgaag aacccgggct cccgggtgac tcacgcggcc
     2761 ggaggcggct gaagcgctga gactctccaa tacgttacgt acgtcatcgc tttcgtcctt
     2821 cgttcccgtc tccccgacaa cctcacgatg tccggcgacg acttctgcgg aagtcctggc
     2881 gatatcctgc ggatgacttc cgtcttcggc tgcgcgccac tgttcggcca tggcgctctt
     2941 gctttcaacg accgcgagat cgggcgcatg ttcgggcaaa cggaccacat ggctcagcca
     3001 ttcagaaata tcggactggc tcagaacggg attgcctatg gtgatattga gacctttata
     3061 accgggatcc agctcttcgg tcgcattgtc cgcgcccgcc gtatggcggc tgttcagagc
     3121 ggcggtgacg ggacgatagg ccgcgagagg tgtgtggagc gtaccggcct gaccggaaga
     3181 ctcggaagat ttggagaggt cacccatgga acccgctgca ggcgcggttt tgcgggcggc
     3241 tatcggatgg actgaaggag cgatcaattc tctgcccgcc atgaagagga tccccgtgac
     3301 caggacagac atactgccga tgaaccattt tcttttcgta tgtaacgagt tgtgaacgcc
     3361 tttccatacg ttttcagaag ggtacatctt gtattgttct gtcttgtccc tgatcagttg
     3421 ctcaaaatcg tcactataga agttgctctc cttcatttgt caatcagttg ttttgctgcc
     3481 ttttcataag taggatttag cggacggcca tcagccactc ggtcttttct ctcggcttcg
     3541 tcagtatctt cttccgtatc aatatctctt cgagcatctg tttggccctt gtatattgcg
     3601 aacgactcgt actttcctcg atatccagca tatcggctat ctctttgtgc gaatagccct
     3661 ctatcgcgta caggttgaga acggtgcggt accctatcgg caggacgcgt atgcattcaa
     3721 ctatctgttt cgcctgcacg atcgacggga cggtctcttc acgcacctga acgccatgtg
     3781 catgaacgat gtccagactc tcgttgaacc gcttgttttt tttcaggttg ttgatacagg
     3841 tatgaacgat gatccgcctg atccaacctt cgaatgcacc tttgttctga aaggtgtgca
     3901 tctgggaaaa cacttttatg aacccttcct gcaacatatc ctccgcgtct tccctgttgt
     3961 gggcaaaccg gtagcagacg gccagcatct tcggactgta cctgttgtac agctcgcgtt
     4021 gggcagccgc ctcatttttt aaacagcctt gtaggatggc ttcttcggtc ataaactaac
     4081 cgcagttggt taccctgtaa tttagacaat tttaacgatg taaagctgcg tactaataaa
     4141 aaaatgaggc ccggatttcg tagaaatccg ggcctgcggc ccgcccccag acccggcctc
     4201 aaatgaggat ctcgccggtc atctccgccg gtatttctaa tcccatcatt gtgaggatgg
     4261 taggcgccag atcacccagc ttgccggacc ggatgccccc tttttggccc ttatcgatga
     4321 cgaaaaaggg cacggggttc atggtatgcg ccgtgttggg ggtgccgtca ccgttgatca
     4381 tataatcggc attcccatgg tccgccgtca gaaaaacgat ataaccacta tccagcgccg
     4441 ccgatacgac cctttccacg caacggtcca ccgtctccgc agccttgatg gccgcccccc
     4501 agacaccggt atggccgacc atatccgcgt tggcatagtt aagacagata aaatccgcgc
     4561 tccgatcccg gatctccgga acgatggcat ccgtcagctc aaccgcgctc atctccggct
     4621 tcagatcgta ggtcgctact ttcggagaag ggaccatgat ccgacgctcg ccctcgaaag
     4681 gcgtctcgcg gccgccgctg aagaaaaagc tgacatgagg atatttttcg gtctctgcga
     4741 tgcgtatctg tttcaaccca tgctccgcga tgacctcgcc gagcgtgttc ttgagatcgt
     4801 cgttttcgaa cattaccccg acccccttga accgcgcgtc gtattgggtc atggttgtat
     4861 aatgaagggc taatttcttc atgccctgct cagggaaatc ctgctgcgta agcacctccg
     4921 tgatctcccg gcatcggtcc gtccggaaat tgaaacaaag cgcatagtcg ccgtcctgta
     4981 tcgtggccag cggcagctga tcgctgccgg caatgacggt gggtttaatg aattcatcgg
     5041 tgatgcccgc ggcataagag ttctcgatcg cagccaatgc atcggttgcc ttttcgccgg
     5101 cgcccttaac gagggcgtca tacgcgagtt tgactctttc ccagcgtttg tcgcggtcca
     5161 tggcatagta ccgcccgctg acggtggcga tcttgccggt ggtgatatta aggtgctcct
     5221 gcaggtcgcg gatatacccc agaccacttt tcggatcggt atcccggccg tccgtaaagg
     5281 catggatgaa cacccgggtc aacccctctt tcagacaggc atccacgatc gctttcagat
     5341 gacggatatg gctatgtacg cccccgtcgc tcaccagacc aagcagatgc agcgcttttc
     5401 cttctttttt ggccccgcgg ataacggaaa ggagcgcggg attttggaaa aaagaacctc
     5461 cccttatggc aagattgatc cgttgtagtt cctggttgac gatacgtccc
//
LOCUS       DASYSV010000224         6912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_678409, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000224 DASYSV010000000
VERSION     DASYSV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678409"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /locus_tag="VGQ51_06935"
     CDS             complement(<1..1935)
                     /locus_tag="VGQ51_06935"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEV9036333.1"
                     /translation="MSRISLIVTYSILSSLYASAQTGGYAYLEFVENKGQWDSSVRFR
                     ADLAAGNLFMQRTGFTVLLRDTTDMRRIGRLLHGDADVLSQFGNTVPRVASGSVAGNV
                     KAATSVSGGQPANNYPVEGGGRTGAGGDPYLLHSFSYRVSFENAGEHVEILPDKPLPS
                     YNNYFRGDRKNWATGCRIYQGILYKNVYDGIDVRYYSDRGFVKYDIIVHPGADPNKIV
                     LKYDGPQKVTLRKNRVYIQTSVATVQELEPHTYQVTDGGRSEVDCDYVLLGGNRIGFR
                     LKNYSHDATLIIDPTEVFCSFSGSKSDNWGYTATYDNSGNFYMGGIVLDETDQSPRGP
                     GGNGYLASPGAFQTSFQGGDGSEGDGYDYDVAIMKLSGNGANRVYATYLGGSGDEQPH
                     SMVVDNSGNLVVTGRTSSANFPPNAPGTLYGNGGGFDLFIAKFNSTGTALIGSKRIGG
                     KGDDGVNYMPKYLINNVSQQGAHDLRLNYGDDGRSEVILDGAGNIYVAACTQSSDFPV
                     TPGVFQSVSGGAQDGVLIKTDPNLTKILFSSLLGGSSNDAAFALSLNPLDNTIWVGGG
                     TESANFPGTGNAPVVSPNGNGGIDGFIAVVSNDGSTLIKSSYFGTALTDMVYGMEFDK
                     NGFPYIMGTTDGLIPVVNSPF"
     gene            complement(2013..4121)
                     /gene="recG"
                     /locus_tag="VGQ51_06940"
     CDS             complement(2013..4121)
                     /gene="recG"
                     /locus_tag="VGQ51_06940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV9036334.1"
                     /translation="MKDIASILRNPIEYLKGVGPLRAELLQKELGIFTFSDLLDHFPY
                     RHVDRTRIQLIRDIRPETDFIQVAGKVVSVEVLGFKQSRRLVAELRDASGTLELVWFQ
                     GINGMQKLLTVGGEYLVYGKAGFFQGSPTITHPEIEVSGGQGAGRPEFLEPVYPTTEK
                     LKVRGLGGRQLGKLVTTLFNILKDSDLPENLPADIREEGWPSRYEAYRQIHFPADLSV
                     YTRAAERLKFEELFLAQLRLGLIKSRRHRYSKGVVFAQVGQLFNQFYTQYLPFELTGA
                     QKRVLREIRNDTGSGRQMNRLLQGDVGSGKTIVALLAMLLALDNGFQACLMAPTEILA
                     RQHYEGIRKLLEEMPVEVRLLTGSSRSKVRKEVLGLAREGGLHILIGTHAVLEESVQF
                     GRLGLAIVDEQHRFGVQQRARLWKKAAVPPHVLVMTATPIPRTLAMTAYGDLDYSVMD
                     ELPPGRQPISTVHRYEDRRPQVMDFLRHEIGKGRQVYVIFPLIEESETLDYENLEKGY
                     ENIKAWFPEPKYWISRVDGRQKQEIRDTNMRRFLGGDTQIMVATTVIEVGVNVPNATV
                     MVVESAEKFGLSQLHQLRGRVGRGAEKSFCILLTSPRLGKEARERLSILTETNNGFKI
                     AEKDLEIRGPGDIEGTRQSGLLSFKLANIVQDRALLELAREKAAVLLDKDPELESEEN
                     RALKLFLQSQQGKTAWSKIS"
     gene            complement(4138..5760)
                     /locus_tag="VGQ51_06945"
     CDS             complement(4138..5760)
                     /locus_tag="VGQ51_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036335.1"
                     /translation="MSRKLVPRIEVFIIVVLACVPLFIRLPYRVNIFLSWEGAYRMSE
                     GQLPFRDFGVPLGGMYWVIPAIFFKLFGPAMITLVKAQVFINIVSGLAFRSILRSLSV
                     NPGIRLASVLLFCLSYSFFNFWPWYNQSVIVFELVGLAFLLRGITGAGMAWLAGAAFF
                     IACSFLTKQDGGGLAFVVACWVLAVNGILQRSWKALAVFAGSFIVLLFLLILPFLSHG
                     FGYWFNHGQAPHSSRVSPYDILNELFGYSQWIKFYLFVIVLLGVLRWRSLPALRSEKP
                     GVLLAAVALGILGEAAVLQVTSYTPPDNNIFFHSFGFACVFTLLAGLVPSLAERRGMV
                     LVLSAGIVLWWSGVWWNYGQRILARIMPPRENATVSPTGEKVVNRDTYMINRDTTKEY
                     PQDQWVECGLPVFRKITMPRPTAEGIHRLMDMDLVRGGRGGLRVLNMSELTPLAEAIP
                     YKLERNPLLPLWFHLGVGMFNRQADTFETRIRDHYYDLVLFEYIPTLNNFYPFRVRDS
                     LRVHYTQVDSFPAPRRGAETVGTIEVFVRPKE"
     gene            5841..6725
                     /locus_tag="VGQ51_06950"
     CDS             5841..6725
                     /locus_tag="VGQ51_06950"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEV9036336.1"
                     /translation="MVYLRLLRPKDWAKNLFLFLPLFFGREIFNIEKLLNVALGFVAF
                     SCIASSIYIINDYRDREDDRKHPVKCKRPLASGAVSPSSALVICGALILAGFALAWIL
                     REKFFVVLAIYFLLNLAYCLGLKAISVLDIMILAAGFVLRIKAGSVISYVPLSEWIII
                     MVFLLAVFMAVGKRRDDVLLKLSSGTDMRKSIKGYNLEFLNILLAFVCAVIVVAYLMY
                     TMSPTTIANTGSSRLYYTCIFVLAGIMRYLQIIYISTDSGSPTKILYKDRFIQITLFL
                     WVISYIVLLYIKNVNIFA"
     gene            6728..>6912
                     /locus_tag="VGQ51_06955"
     CDS             6728..>6912
                     /locus_tag="VGQ51_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEV9036337.1"
                     /translation="MPSVLILGAASDIAVSLARKYGSMGFAVQLAARNASRLQPLQLD
                     ISIRYSVACTVHEFDAA"
BASE COUNT         1593 a         2222 c         1536 g         1561 t
ORIGIN      
        1 gaagggactg ttcaccacag gtatcaatcc atcagtcgtt cccatgatat agggaaaccc
       61 atttttgtcg aattccatcc catagaccat atcggtaagc gccgtgccga aataagacga
      121 tttgatcagg gtagacccgt cattgctgac gaccgcaatg aatccgtcga tacccccgtt
      181 gccgttggga ctaaccaccg gcgcattccc ggttcccgga aaattggcgc tctccgttcc
      241 accacccacc cagatcgtat tgtccagcgg attcaacgac agcgcaaacg ccgcgtcgtt
      301 gctgctccca cccaacaggc tgctgaaaag gattttcgtg agatttgggt ccgtcttgat
      361 aagcaccccg tcctgcgcgc ccccggaaac actctgaaaa acacccgggg tcactgggaa
      421 atctgaggat tgggtgcagg ccgcgacata aatatttccc gcaccatcga ggatcacttc
      481 gctccgcccg tcatctccgt aattcagacg caggtcatgc gcgccttgct gcgagacatt
      541 attgataagg tatttgggca tatagttcac accgtcgtcc cccttcccgc cgattcgctt
      601 gctgccgatg agcgccgtgc cggtagaatt gaacttggca ataaaaagat cgaaaccacc
      661 cccattgcca tacagagtgc ccggcgcatt cggagggaaa ttggccgagc tggtccgccc
      721 ggttaccaca agatttccac tgttgtcaac gaccatgcta tgtggttgct cgtcaccgct
      781 gccgccaaga taagtagcat acacgcggtt ggcgccattg ccgctcagct tcatgatcgc
      841 tacgtcgtaa tcatacccgt ccccttcact tccatcccca ccctgaaagc tcgtctggaa
      901 cgcgcccgga ctcgccaggt aaccgttgcc gcccggcccc cgcggactct gatccgtttc
      961 atccagcacg atcccgccca tatagaaatt gcccgagttg tcataggtgg cggtataccc
     1021 ccagttatcg gatttgctgc ctgaaaaaga gcagaaaacc tccgtaggat cgatgatcag
     1081 tgtggcgtca tgtgaataat tctttaacct gaacccgata cggttgccgc ccagcaggac
     1141 ataatcgcaa tctacttcac tcctcccccc gtccgtcacc tggtaggtat gcggctccag
     1201 ttcctgcacg gtcgcgacgg acgtctggat atataccctg ttcttccgta aggttacctt
     1261 ctgcggcccg tcgtatttga ggacgatctt gttcgggtcg gcgccggggt gcacgatgat
     1321 gtcgtatttg acaaaccccc tgtccgaata ataccggacg tcgataccgt cataaacgtt
     1381 cttatacagg ataccctgat agatcctgca acccgtggcc cagttcttcc tgtcccccct
     1441 gaaataattg ttgtaagatg gcagcggttt gtccggcagg atctccacat gctcacccgc
     1501 attctcgaaa ctcaccctat aagaaaaaga atgcaaaaga tacggatcgc cgcccgcccc
     1561 ggtcctccct cccccttcca ccggataatt attcgccggc tgcccccccg acaccgatgt
     1621 tgcggcctta acattgccgg caacactccc cgacgccacc ctcggcaccg tatttccaaa
     1681 ttggcttaaa acatccgcat ccccgtgaag aagccttcct atccgccgca tatcggtcgt
     1741 atcccgaagc aggaccgtaa aacccgtccg ctgcatgaac agattgcccg cagcaagatc
     1801 ggccctgaaa cgcaccgaac tgtcccattg ccccttattc tcgacaaatt caagataagc
     1861 atagcctcct gtctgtgccg acgcataaag tgacgataaa attgagtagg taacaataag
     1921 acttattctg ctcaattggc tggttttcaa ttaattaatt ccgtcccgtc tcacgccgta
     1981 tttcccccgg ccgcgccaaa acccggtgag gcctacgaga tcttgctcca tgccgtcttg
     2041 ccctgttgcg attgcaaaaa cagcttcagc gccctgttct cttccgactc cagctcagga
     2101 tccttatcca gcagtactgc cgccttttcc ctggccaatt ccaacaacgc tctgtcttgc
     2161 acgatattgg ccaatttaaa gctcaacaga ccgctttggc gggtaccttc gatatctccc
     2221 ggcccgcgga tttccaggtc cttttcagca attttaaagc cattattagt ttcggtgagg
     2281 atgctcagtc gctccctcgc ctccttcccg agacggggag atgttaataa gatgcaaaag
     2341 ctcttttcgg ccccccggcc gacccttccc ctcagctgat gcagctggga taacccgaat
     2401 ttctcggcac tctcgaccac catgacggtc gcattcggaa cattcacgcc aacttcgatc
     2461 accgtggtgg ccaccatgat ctgtgtatcg ccacccagaa aacgccgcat gttcgtatcc
     2521 ctgatctcct gcttctgcct cccgtctacc ctgctgatcc aatacttcgg ttccgggaac
     2581 cacgctttta tgttctcata acccttttcc aggttctcat aatccaacgt ctccgactct
     2641 tctatcaggg gaaatatgac atacacctgc cgcccttttc cgatctcatg ccgaaggaaa
     2701 tccatcacct gcggtctcct gtcttcgtaa cgatgaaccg tcgatattgg ttgccttccg
     2761 ggaggcaatt cgtccattac actgtagtcc agatcgccat aggcggtcat cgccagcgtc
     2821 ctcggtatcg gggtggccgt catgaccagc acatgcggcg gaacggctgc tttcttccag
     2881 agacgcgcac gctgctgaac gccgaaccgg tgctgctcat ccacgatcgc caatcccagc
     2941 ctgccgaact gcacggattc ttccagcaca gcatgcgtcc cgataaggat atgcagcccc
     3001 ccctcccgcg ccagcccaag cacctccttt ctcactttcg accgggagga acccgtcaac
     3061 agccggacct ccaccggcat ctcttccaaa agcttcctga tcccttcata atgctgacgc
     3121 gccaggatct ccgtcggcgc catcagacag gcctgaaacc cgttgtcgag cgccaacaac
     3181 atcgccagca acgccactat cgtcttgcca ctccctacat cgccctgcaa cagccggttc
     3241 atctgacgcc cgctgcccgt gtcgttcctg atctcccgta ataccctctt ttgtgccccc
     3301 gtcagctcga acggcaggta ttgtgtgtaa aattggttga atagctgccc cacctgcgca
     3361 aataccacac ctttggaata ccggtgccgc ctcgatttga tcaaccccag ccgcagctgc
     3421 gccaggaaca gctcctcgaa tttcagccgc tcggcagccc tcgtgtagac cgacagatcg
     3481 gccggaaaat ggatctgccg gtaggcctca taacgcgacg gccatccctc ttcccgtata
     3541 tccgcgggca aattctctgg taagtcgcta tctttcaata tattaaaaag ggttgtcacc
     3601 agcttgccca gctgcctgcc ccccaatccc cggaccttca gcttctccgt agttggatac
     3661 accggctcca aaaattccgg ccgccccgct ccctgcccgc ccgagacctc tatctccgga
     3721 tgcgtgatgg tcggactgcc ctgaaaaaat ccggccttcc catacaccag gtactcccct
     3781 cccacggtca acagcttttg catcccgttg atcccctgga accacaccaa ctccaacgtc
     3841 ccggacgcat cccgcagttc ggccaccagc cgccggcttt gtttgaaccc cagcacctcc
     3901 accgaaacaa ccttccccgc gacctgaata aaatccgttt ccggacgtat atcccggatc
     3961 agctgtatcc gggtacgatc cacatgccgg taagggaagt gatccaacag atcggaaaaa
     4021 gtgaagatgc ctaattcctt ttgcagcaat tcggcgcgca agggccccac acccttcagg
     4081 tattcaatgg gattccgcaa tatagaagca atgtctttca tggtaccact caattcgcta
     4141 ttccttcggc cgcacgaaca cttcgatcgt gcccacagtc tcggcccccc tcctgggcgc
     4201 gggaaaagag tctacctggg tataatgcac acgcagcgaa tcccgcaccc gaaatggata
     4261 aaagttattc aaagtcggaa tatactcgaa tagcacgaga tcgtagtaat gatcccggat
     4321 acgcgtctcg aacgtgtccg cctgacgatt gaacatgccg accccgagat gaaaccacaa
     4381 aggcaaaaga gggttccttt ccaatttata tggaatggcc tcggccaacg gcgtcagctc
     4441 cgacatattc agcaccctca gacctccccg gccacccctg acaagatcca tgtccatcaa
     4501 ccggtggata ccctccgcag taggcctcgg catagtgatc ttccggaata cgggaagccc
     4561 gcactccacc cactgatcct gcggatattc tttggtcgta tccctgttga tcatataagt
     4621 atccctgttc acgactttct cccccgtcgg actgacagtg gcattctccc gcggaggcat
     4681 gatcctcgcc agtatgcgtt gcccatagtt ccaccagacg ccgctccacc acaagacgat
     4741 cccagccgac aataccagca ccatccccct cctctcggcc agcgaaggta ccaaccctgc
     4801 cagcaaagta aacacgcagg cgaaaccgaa agaatggaaa aagatattgt tgtccggcgg
     4861 agtatagctc gttacctgca ataccgccgc ttcgccaagg atgcccaacg ccaccgccgc
     4921 aagcaacacc cccggctttt ccgaacgcag cgccggcaga cttcgccaac gcaacacccc
     4981 aagcaggacg atcacgaaca ggtagaattt gatccattgc gaatacccga aaagctcgtt
     5041 caatatatcg tatggcgata cccgtgagga atgcggagcc tgaccatgat tgaaccaata
     5101 cccgaaccca tgcgaaagga atggaaggat cagcaaaaaa agcaaaacga taaagcttcc
     5161 cgcgaacacc gccagcgcct tccagcttcg ctgcaatatc ccattcaccg ccaataccca
     5221 gcaggcgacg acaaaggcca gtcctcctcc atcctgcttg gtcaggaaag aacaggcgat
     5281 aaaaaacgcc gcccccgcca gccaggccat ccccgccccg gtgatccccc gcaacaggaa
     5341 cgccagtccc accagttcga agacgataac ggactgattg taccagggcc agaaattgaa
     5401 aaaggaatac gagaggcaaa acaacaacac ggaagccagc cgtatcccgg ggttgacgga
     5461 gagactgcgc aatatcgacc ggaatgccag ccccgacaca atattgatga agacctgcgc
     5521 tttcaccaac gttatcatcg ccggtccgaa caatttgaaa aagatagccg gtatcaccca
     5581 atacatcccg ccgagcggca cgccgaaatc cctgaagggc agctgccctt cgctcatgcg
     5641 ataggcgcct tcccaggaaa gaaagatatt cacccgataa ggcaaccgga tgaacaatgg
     5701 aacacacgct aatacgacga tgatgaatac ttcaattctt gggaccagct ttcggctcat
     5761 gaggttatct tattattatt cggagacaat attataaatt ttgtcttttt gtttgtactt
     5821 tggcgcgtta aataaatgaa gtggtatacc tccgactact cagacctaag gattgggcaa
     5881 agaatctgtt cctttttctg cctctatttt tcggccgtga gatattcaat atcgaaaagc
     5941 tgctcaacgt ggcgcttggt ttcgtcgctt tctcctgtat cgcgagcagt atctatatca
     6001 tcaatgatta ccgtgacagg gaagacgacc ggaaacatcc cgtcaaatgc aaacggcccc
     6061 tggcatcggg agcagtctct ccgtcttcgg ctctggtcat ctgcggcgcg ctgatccttg
     6121 ccggttttgc cctcgcctgg atcttacggg agaaattctt cgtcgtactg gccatctatt
     6181 tcctgctcaa tctcgcctat tgtctcggcc tcaaggccat ctcggtgctc gatatcatga
     6241 tcctggccgc aggcttcgtc cttcgcatca aagcaggctc cgtcatttcc tatgtgcccc
     6301 tgtccgaatg gatcatcatc atggtcttcc tcctcgccgt cttcatggct gttggcaaac
     6361 gacgtgacga tgtcctcctc aagctcagct cgggcaccga tatgcgtaaa tccatcaagg
     6421 gatataacct cgaatttctc aatatattgc tcgctttcgt ctgcgccgtc atcgtcgtcg
     6481 cctacctgat gtacaccatg tcgccgacca ccatcgccaa taccggttcc tccagactct
     6541 actatacctg tatcttcgtg ctggcaggga tcatgcggta tctgcagatc atctacatct
     6601 ccacggactc cgggtccccg acgaagatcc tgtataaaga ccgctttatt cagatcaccc
     6661 tgttcctgtg ggtaataagc tacatcgtgt tattgtacat caagaacgtc aatatattcg
     6721 cttaaatatg ccctctgtac tcatactcgg cgccgcttcc gatatcgccg tctcccttgc
     6781 ccggaaatac gggtccatgg gtttcgcggt acagctggcc gcgcgaaacg cctcacgtct
     6841 gcagcctctg cagctcgata tctccatccg ttacagcgtc gcctgtacgg tccatgagtt
     6901 cgatgcggcc ca
//
LOCUS       DASYSV010000225        13532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1703324, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000225 DASYSV010000000
VERSION     DASYSV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703324"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VGQ51_06960"
     CDS             complement(<1..549)
                     /locus_tag="VGQ51_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEV9036338.1"
                     /translation="MKLFKRRTRNGDDQEMSFIEHLDVLRGHLFKSALAVAVGAIVMA
                     VYNKFIVQHILMGPTHPDFWTYKTLCEVGHKWGLGDKLCMAEIHLRMQSNAVAGQFDT
                     YFNIILIGGFILAFPYVFWQFWKFTKPALKEKELRNTRGVIFWVSMLFFIGVLFGYFI
                     IAPYTISFFANFSLDTNIENIWT"
     gene            complement(656..889)
                     /locus_tag="VGQ51_06965"
     CDS             complement(656..889)
                     /locus_tag="VGQ51_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEV9036339.1"
                     /translation="MGDLFSGPHIILIALVVLLLFGGKKIPELMRGLGKGIREFKDAK
                     DNVQKEIEDHVNDDRKTTTTTTTTKVTSTPDHN"
     gene            complement(951..1322)
                     /gene="rplS"
                     /locus_tag="VGQ51_06970"
     CDS             complement(951..1322)
                     /gene="rplS"
                     /locus_tag="VGQ51_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV9036340.1"
                     /translation="MNAVAFVHEQLTGKKSLPEFKAGDNITVNYKIIEGNKERIQSFK
                     GDVIKRQGTGHTATFTVRKISDGVGVERTFPLFSPNIESLEMNKVGRVRRAKLYFQRS
                     RSGKSARIKEKRMAVGDAAGK"
     gene            complement(1424..2980)
                     /gene="rny"
                     /locus_tag="VGQ51_06975"
     CDS             complement(1424..2980)
                     /gene="rny"
                     /locus_tag="VGQ51_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV9036341.1"
                     /translation="MLYTVIAAVIALVVGIVAGKLLFAKNTRQKIEDAESQAKKIVSD
                     AQTSAESLKREKQLEAKEKFVQLKAEHDKEVLDRNRKINDSEARIKQKEQSINQKLEN
                     LDKQAKDNDAIKENLNRQIEVVNHKRTELEKHQEEHIRRLEKIAALTAEEAKTQLVES
                     LKQEAQTRALALQQEIIDDAKQKANKEARKIIIQSIQRTAAEQAIENSITVFNLESDE
                     IKGQIIGREGRNIRAIEAATGVDLIVDDTPEAIVLSSFDPLRREIARLSLQRLVADGR
                     IHPARIEEIVEKTRKQIEEQVMEIGERTVIELGIHGLHKELVRIVGKMRFRSSYGQNL
                     LMHSREVANLCGIMAAELGMNPKLAKRAGLLHDIGKVPDEETELSHALLGMKLAEKYG
                     ENPAVINAIGAHHDEVEMQYVIAPIIQACDAISGARPGARREIMQQYLQRIKDLENLA
                     LAYPGVEKAYAIQAGRELRVIVEADKVTDVDSDKLSFEIAQKIQTEMTYPGQIKVTVI
                     REKRAVNVAR"
     gene            complement(3087..3371)
                     /locus_tag="VGQ51_06980"
     CDS             complement(3087..3371)
                     /locus_tag="VGQ51_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV9036342.1"
                     /translation="MQELIPINVVIGDRTYRIKIEPRDEEVVRRTLKQINDKIIEFKT
                     QFAGRDMQDYIAMVVLWYATLPAGQNGGMGPDPSVLDALARMEQQLDKVL"
     gene            3484..3738
                     /locus_tag="VGQ51_06985"
     CDS             3484..3738
                     /locus_tag="VGQ51_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036343.1"
                     /translation="MFLKVKSPVFIDHLTFQVDHPQKCLLKAVLKDDTGLVCSALETE
                     VENDQQELAWNGLNDLPYGVYTLEISRGQDEMKMRLVKRV"
     gene            complement(3744..4052)
                     /locus_tag="VGQ51_06990"
     CDS             complement(3744..4052)
                     /locus_tag="VGQ51_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036344.1"
                     /translation="MSDDTNNAGETELRVRRIQDKLQQLLRQRESLQKENGRLKEDMR
                     QLQADLSERTTRLEQLQQQVEILRATKAAMSEEEKRALEKRLAQYIREIDRCIAMLGE
                     "
     gene            complement(4090..6564)
                     /gene="pheT"
                     /locus_tag="VGQ51_06995"
     CDS             complement(4090..6564)
                     /gene="pheT"
                     /locus_tag="VGQ51_06995"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV9036345.1"
                     /translation="MTISYNWLCDYLPVAIEPEKLSKILTSIGLEVEGVERYESVRGG
                     LKGLVIGEVLECGPHPNADKLRLTKVGIGGKESLSIVCGASNVGAGQKVVVALPGVTI
                     YPTKGEPLTLKVAKIRGVESHGMICAEDEIGLGEEHAGILVLPADVEPGTPAEDWFKP
                     YTDYLISIGLTPNHMDAMSHWGVARDVCAWLSHHEQKEYRPKEPRTAGQAEGLALGLA
                     AEAAGYAAGQASTVKVSILDTHGCARFSGVTLKGITVRESPRWMQDRLKAIGIRSINN
                     IVDITNYVLHEMGQPLHAYDLNEIGGKQIIVKHLPEGTSFTTLDEKERKLSAEDLMIC
                     NADGPMGIAGVFGGLHSGIKSGTTDIFLESAWFNPTDIRKTSFRLGLRTDAAVHFEKG
                     MDISGTVNALKRAALLILEMAGGAPASEIVDVYPEPGRMEEVTLKYAYLRKLSGKNYT
                     PDVAKNILVALGFGLVKESDDEVRVAVPWHKPDISLPADLVEEIMRIDGLDNIAIPKT
                     ITISPSVEKDASAAAHRTAIAHWLVGQGFYEILTNSITNSAYFSESELETTVKMVNNL
                     SAELDVLRPSLLETGLESIAFNLNRKTSGLRFFEFGRTYRTGGVGDYVETNHLCLYLT
                     GPVREDSWKGKGKPMDFFYVKGLCERLFEVLGVPFAGWIEQPVAKLTSGLSVRLDKDI
                     LLEAGSVRPESLKRFDIRQEVFYVDIRWDILIGNASKRKIEFRELPRQLPVYRDLAMV
                     VDKSLSYGRVEEAVRTVRLDKLQEVRLFDIFENEEKLGKGKKSLAVSFTFLDEEKTLT
                     DKEIDGMIARIMSALEKEVQAEIRTQ"
     gene            6863..8533
                     /locus_tag="VGQ51_07000"
     CDS             6863..8533
                     /locus_tag="VGQ51_07000"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEV9036346.1"
                     /translation="MKKRLLYLCIGALALHACGPVYVVRQPTPQPAPATPPPADPEDV
                     SYQTFYDQLSPYGQWVEDPEYGYVWMPSVGEDFKPYASNGHWVYTDEGWTWDSGYPWG
                     WAAFHYGRWFFKDGYGWEWIPGNEWAPAWVAWRNSDDYYGWAPLGPSVSLTVAYDGGY
                     NPPPHYWCFVPHQYVSSPQLGNYYVRETNNVTIINRTTIIRNTTVINNNRNRHYGYGP
                     DAGEVGRYSGSQVHPISIHEIRTPGGQATNGSFAVYRPHVNSAPAGNGNQGNNGRPAY
                     APTHYQSINNVRPVNRTNYGNPGGQGNSGSEAGTRNNNGNNGNHNGWNNNNGNNGNGN
                     NGNGNNGNGNNGNGNVNNSNGNNGNGNNGNHNGWNNNNGNNGNGNNGNGNVNNSNGNN
                     GNGNNGNGNNGNGNGNNGNGNGNNGNGNGNNGNHNGWNNRSGNPTQSNTPPANTPAVN
                     TPPANTPPANNPPANTQQGRPTTFPGAPSRQTNNPNNGQPQHVFRSNPTLTQQQSTSG
                     RQPQGRPNAQPGNSNQSRPVGNSVKPAPQQQRPPHPPGRPDSKPDNKS"
     gene            complement(8639..8944)
                     /locus_tag="VGQ51_07005"
     CDS             complement(8639..8944)
                     /locus_tag="VGQ51_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036347.1"
                     /translation="MTQLAIITELLRQHEGNVTRFVREANIDCLKPPDNNFLFVSLSF
                     RNPLGYSQEIGAYAQYSATYPQFKKVFNPFEINIHTSCAQVFDRKNFAGKVKLQNEV"
     gene            9067..10563
                     /gene="dnaB"
                     /locus_tag="VGQ51_07010"
     CDS             9067..10563
                     /gene="dnaB"
                     /locus_tag="VGQ51_07010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV9036348.1"
                     /translation="MVYGKIPPQAKELEEAVLGAIMLETSAFDTVVEILKPECFYLEG
                     HQRIYRAMQGLAQKSQPIDILTVVEELRTRSELDIVGGPYYVTKLTNSVVSTANIETH
                     SRIILQKFIQRELIRISGEIIGEAYEDSTDVFDLLDDAESKLFEITNNHLRKNFDSID
                     TVLVKTIQRIEDLRHRQDEITGVPSGFASLDKVTYGWQSTDLIILAARPAVGKTAFAL
                     NLARNAALHPTKPTPVAFFSLEMSSAQLVQRILSAESEIFMEKIARGRLEEHEMKQLY
                     KKGIDRLAKAPIFIDDTPALNIFELRAKCRRLKNKHHVGCIIIDYLQLMSGAGDNKNG
                     NREQEISRISRDLKGLAKELQVPIIALSQLSRAVESRKEGEKIPQLSDLRESGAIEQD
                     ADMVMFLYRPEYYGITANENGESNKGETHVKIAKHRNGSLETIKLRALLHIQKFTEYG
                     DDDFGGIGMPGGGNWKPVQDNEGGGARLYIQAGSRMKDLPVDDDETPF"
     gene            10568..11275
                     /locus_tag="VGQ51_07015"
     CDS             10568..11275
                     /locus_tag="VGQ51_07015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEV9036349.1"
                     /translation="MALPFFYITAYVADQQELILDEGNSRHVVQVLRMKPGESLRLTD
                     GKGALLTAEIIDDHKKKCRVSILTATFQPPPERKVTIAISPLKNASRFEWFLEKATEI
                     GVSAVIPLVCERTERPSIRHDRLQQIAVSALLQSQQAWLPDLAAPIPLADLLPNSTIR
                     NRYIAHCLEAPCPTVSLTDTPQADHSLILIGPEGDFSIKEIENALAAGFIPVTLGPNR
                     LRTETAGVVAATLLCIA"
     gene            complement(11278..11889)
                     /locus_tag="VGQ51_07020"
     CDS             complement(11278..11889)
                     /locus_tag="VGQ51_07020"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEV9036350.1"
                     /translation="MPATDARIDAYIAKSADFAKPILNHLRKLVHKACPDATETIKWG
                     MPFFETSGSILCNIAAFKEHCSFGLWNAPLLKDPEGILQVKDKNAMGHFDRITSLKDL
                     PADKIIIAYLKEGASLNEKGVKKPVAKKAPKPELPVPPELAAALKKNKAAKAAFEGFS
                     PSHRREYIEWIVEAKTDATKEKRVATTIEWLVEGKSRMWKYQK"
     gene            complement(11921..12646)
                     /locus_tag="VGQ51_07025"
     CDS             complement(11921..12646)
                     /locus_tag="VGQ51_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEV9036351.1"
                     /translation="MHQLFSWDSLVSLVTLIVLEIVLGIDNVIFVSIIIGRLPSHQQL
                     AARRVWMVAGSLVRSGLLLGLGWLVTSGNTKLFSLAGYGFSTRNLIMTAGGLFLLYKT
                     VREIHEKLEGDPQEGEEATDEENPSFWGLVAQLVLVDTVFSFDSIVTAVGLAQQIAVM
                     ITAVVLAMILMFFFSRSIANFINRRPTVKILALSFLLMIGFSLLFEGLEPLHHMHIDK
                     GYIYFSMAFAFGVELLNSRVRKK"
     gene            complement(12681..>13532)
                     /locus_tag="VGQ51_07030"
     CDS             complement(12681..>13532)
                     /locus_tag="VGQ51_07030"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEV9036352.1"
                     /translation="GFDKNALYLRELDALGFGFVEIGTVTPRVQEGNPRPRLFRLPED
                     QGLINRMGFNNDGVEAVAARVRAWRSSRAGRNSGLIVGGNIGKNKVTPNEDAWKDYEI
                     CFRALYDAVDYFVVNVSSPNTPGLRELQEKDSLRKILVNLQGVNREMSGVLRVRPRPL
                     LLKIAPDLSSSQLDDVVDLAVEIGLDGLVATNTTISRDGLLTSAGRVAEIGAGGLSGA
                     PVRELSTEVVRYCCARLGGRIPVIASGGIFTVADMREKQGAGAVLVQVWTGFIYEGPF
                     VVRRICR"
BASE COUNT         3173 a         4022 c         3202 g         3135 t
ORIGIN      
        1 cgtccagata ttttcgatat tggtgtccag cgagaaattg gcaaagaagc taatggtata
       61 gggcgcgatg atgaaatagc cgaagagcac accgatgaag aataacatcg agacccaaaa
      121 gataacgccg cgggtgttcc ggagctcctt ctctttcaag gccggtttcg tgaatttcca
      181 aaactgccag aagacatagg ggaaggcaag gatgaaaccg ccgatgagaa tgatattgaa
      241 ataggtatcg aattgtccgg ccaccgcatt gctttgcatc ctgaggtgga tctcggccat
      301 gcacaatttg tcgcctaacc cccatttgtg gcccacttcg cataaggtct tgtacgtcca
      361 gaagtcggga tgcgtcgggc ccatcaggat gtgctgaacg ataaacttat tgtatacagc
      421 catcacgatc gctccgacgg cgacagcaag ggccgattta aaaagatgcc cgcgcaacac
      481 gtccaaatgc tcgataaagg acatctcctg atcgtccccg ttcctggtcc ttctcttgaa
      541 tagcttcatt gaaattacaa gtagcgcaaa ttattacaaa aaagaaaacc ccgcagcaat
      601 tcacgcgggg tttattataa agactttgaa gatctttaat gaccggctaa ttatttcaat
      661 tgtggtcggg agttgacgtt accttggtgg tcgtcgtcgt tgtagtggtc tttctgtcgt
      721 cgttgacgtg gtcttctatc tccttctgaa cattatcctt tgcatccttg aattcgcgga
      781 ttccctttcc aagcccacgc atcagttcag ggatcttttt gccaccaaac aacagcagca
      841 ctacaagtgc gataagtata atatgcggac cgctgaataa atctcccata acttcatttt
      901 aggtacttta caaaggtata ccaaaaatcc gtaacaaaaa cataatttgg ctacttgccg
      961 gcagcatcgc cgacagccat tctcttctcc ttgatacggg cgctcttgcc gctacgagac
     1021 cgctgaaaat acagcttcgc gcgacgtacc cgtcccacct tattcatttc taaggattcg
     1081 atattggggg agaacagggg gaaggtcctc tcgacgccaa cgccgtcgga gatcttgcgc
     1141 acggtaaacg tcgccgtatg ccccgtaccc tgacgcttga tcacatcacc tttgaaactc
     1201 tggatacgct ccttgttccc ttcgatgatc ttatagttga cggtgatatt gtctccggcc
     1261 ttgaattccg gaaggctttt tttacccgtc agttgctcgt gtacaaaggc tacagcgttc
     1321 ataataatac tttttaaagg atggcaaagg tagcaacaat cgccataaag tccaaacttt
     1381 tttccctccg gcgtccggtg ctcaccgccg gggatccggt gaattaccgc gctacgttca
     1441 cagcccgctt ttcccggatg acggtaactt ttatctgtcc gggataggtc atttccgtct
     1501 gtattttctg cgcgatctcg aagctcagct tgtcactgtc cacgtcggtc actttatcgg
     1561 cctccacgat gacccgtagc tcacgtccgg cctggatagc ataggccttt tccacaccgg
     1621 gatatgcaag cgccagattc tcaaggtctt ttatccgctg caggtattgc tgcatgatct
     1681 cgcgccgtgc gccgggccgg gccccgctga tggcgtcgca ggcctggatg atgggcgcaa
     1741 tgacgtattg catttcgact tcatcgtgat gggcaccgat agcgttgatg accgccggat
     1801 tctcgccata tttttccgca agtttcattc ccagcagggc atggctcaat tcagtttctt
     1861 cgtcgggtac cttaccgata tcgtgcagca gaccggccct ttttgccagt ttgggattca
     1921 tccccagctc ggccgccatg ataccgcaaa gattggcgac ctcccggcta tgcatcagca
     1981 ggttctgtcc ataggacgac cggaaacgca tctttccgac gatacgcacc agctccttat
     2041 gcaacccatg gatacccaac tcgatcacgg tcctttcccc gatctccatg acctgctcct
     2101 caatttgctt acgcgttttt tcaactatct cctcgattcg ggcaggatgg atacggccat
     2161 cggctacgag ccgctgcagg gaaagccgcg caatctccct gcgcagcggg tcaaacgatg
     2221 ataagactat ggcctccggc gtatcgtcta cgataagatc cacgcccgtg gcggcttcga
     2281 tggcacgtat attccgacct tcccggccga tgatctgacc tttgatctcg tcgctttcca
     2341 aattgaaaac agtaatggaa ttttcgatcg cctgctcggc agcggtccgc tggatacttt
     2401 gaatgatgat ctttctggcc tctttgttcg ctttctgctt ggcgtcgtcg atgatctcct
     2461 gctgcaacgc cagggcccgt gtctgggctt cctgtttcag gctctccacc agctgcgtct
     2521 tggcttcctc ggcggtcagg gcggctatct tctccagacg gcggatatgc tcttcctggt
     2581 gcttttccag ttcggtccgt ttgtgattga ctacctcgat ctggcggttc aggttctcct
     2641 tgatcgcgtc attatccttg gcctgtttgt cgagattttc cagtttctga ttgatactct
     2701 gttctttctg cttgatccgg gcctccgaat cattgatctt ccggttcctg tccagcacct
     2761 ctttgtcgtg ctccgccttc agttgtacga atttctcctt ggcttccagc tgtttttccc
     2821 ttttcaggct ctcggcggac gtctgggcat cggagacgat ctttttggcc tgagattctg
     2881 catcctctat cttttgacgc gtgttcttcg cgaatagtag cttaccggcg acaattccca
     2941 cgaccagggc tataactgcc gctataacag tatataacat tatatattga atttaatatg
     3001 tgcataaaga ccaacttatt ggtccgtttt ttgtgtcaat gggcgaagat acggaataac
     3061 gattaaaaat ccgctggggc ggaatgtcat aaaaccttgt ccaactgctg ttccatgcgc
     3121 gccagggcat ccaggaccga ggggtcggga cccatacccc cgttctgtcc ggcaggcagg
     3181 gtagcatacc aaaggacgac catggcaata taatcctgca tgtcccggcc ggcaaactgg
     3241 gtcttgaact cgatgatctt atcattgatc tgtttgaggg tcctgcggac gacctcctcg
     3301 tcccggggtt cgatcttgat ccggtaggtc cggtcgccta ttacaacatt tattggaatt
     3361 aactcttgca tagttgaaat acttgtttta tatttattca cactttatta acagccaata
     3421 tctaccatcc ttataaaaca acgagcatta ccaccaatac cctatctaaa aactacaaaa
     3481 gccatgttct taaaagttaa aagcccggtc ttcatcgacc atctgacctt tcaggtcgac
     3541 catccacaga aatgcctatt gaaagccgtt ctcaaagacg acaccggtct ggtttgcagc
     3601 gccctcgaga ccgaagttga aaatgaccaa caggagttgg catggaacgg tctcaacgat
     3661 ctgccttacg gcgtgtatac cctggaaatt tcacgtggtc aggacgaaat gaaaatgcgt
     3721 ctcgtcaaac gcgtctgagg tcatcattcg cccagcattg caatacaccg gtcgatttcc
     3781 cggatgtact gggcaagcct tttttccaac gcccgttttt cttcctcgct catggctgct
     3841 tttgtagccc tcaatatctc tacctgctgt tgtaattgct ccagtctcgt agtgcgctcc
     3901 gacaggtctg cctgcaactg acgcatatcc tccttcaatc gcccattctc tttctgcaac
     3961 gactcacgtt gtcgcagcag ttgctgcaac ttatcctgga tccgccggac ccggagttcg
     4021 gtttcacctg cattattggt atcgtcgctc atttttactt gtcccgggcc cccgccatcg
     4081 ctcattctat cattgtgtcc ggatctcggc ctgcacctcc ttctccagcg cactcatgat
     4141 cctcgcgatc atcccgtcga tctctttatc ggtcagcgtc ttttcctcat ccaggaaagt
     4201 aaagctgacg gccagcgatt tctttccctt tcccagtttc tcctcattct caaatatatc
     4261 gaaaagacgc acttcctgca atttatccag cctgacggtc ctgaccgcct cttccacccg
     4321 gccatacgac aacgatttgt caacgaccat cgccagatcg cgataaacgg gcaactgccg
     4381 cggcaactcg cgaaactcga tcttcctctt agaagcgttg cctatcagga tatcccatcg
     4441 tatgtcgaca tagaacacct cctgccggat atcgaaccgc ttcaacgatt caggacgcac
     4501 acttccggcc tccaatagga tatccttgtc taaccttacc gaaagtccgc tcgtcagctt
     4561 ggcgacaggt tgctctatcc atcccgcgaa aggcaccccc aatacttcaa acaaccgttc
     4621 gcacaatcct ttcacataaa agaaatccat cggcttcccc tttcccttcc aggaatcttc
     4681 ccgcacggga ccggtgaggt acaaacagag gtggttcgtc tcgacatagt cgccgactcc
     4741 accggtgcga taggtcctgc cgaattcgaa gaaacgaagt ccgctggtct tgcgattgag
     4801 attgaaagca atggactcca gcccggtctc caacagcgaa ggacggagga catccagctc
     4861 tgcactcagg ttattgacca ttttaaccgt cgtctccagc tcgctttcgc tgaagtaagc
     4921 gctgttcgtg atcgagttgg tgagtatttc ataaaaaccc tgtcctacca gccagtgcgc
     4981 tatcgccgtc cgatgcgcgg ccgcagaagc gtccttctct acggagggag agatggtgat
     5041 ggtcttcggg atggcgatat tgtccaggcc gtcgatccgc atgatctctt ctaccaggtc
     5101 ggccggcaaa ctgatatcgg gtttatgcca gggtacggca acccggacct catcgtcact
     5161 ttccttcacc agcccgaaac ccagcgcaac caggatattc tttgccacat cgggggtata
     5221 gttcttgccg ctcagtttcc tcagataggc atacttcagc gtgacctctt ccatacggcc
     5281 cggttcggga tagacatcca cgatctccga agccggcgca ccgccggcca tttcaaggat
     5341 cagcagcgct gctcgtttca gagcatttac tgtgccggaa atatccatcc ccttctcgaa
     5401 atgtaccgcg gcatcggtgc gcagtccaag ccggaaagat gttttacgga tgtccgtggg
     5461 attgaaccag gcgctttcga gaaatatatc ggttgtaccg gatttgatcc cgctgtgcaa
     5521 tcccccgaat acgcctgcaa tacccatcgg cccatcggca ttgcagatca tcaggtcctc
     5581 cgccgacagc ttacgctcct tttcatcgag ggtggtaaag gacgttccct cgggcagatg
     5641 tttgacgatg atctgtttgc ccccgatctc gttcagatcg taggcatgta acggttgccc
     5701 catctcatgc aggacatagt tggtgatgtc tactatattg ttgatgctgc ggatgccaat
     5761 ggcttttagc cgatcctgca tccagcgcgg tgattcccgg acggtaatgc ctttgagagt
     5821 gacgccggaa aaccgtgcgc aaccgtgagt gtccaggatg ctcactttca ccgtcgatgc
     5881 ctgccccgcc gcgtacccgg ccgcctccgc cgccagcccc aacgccagcc cctctgcctg
     5941 ccccgccgtc cgcggttctt tcggcctgta ttccttctgc tcatggtgcg aaagccaggc
     6001 gcacacatcc ctggctaccc cccaatgact catggcatcc atatggttcg gagtaagacc
     6061 gatcgatatc agatagtcag tataaggttt gaaccagtcc tccgccggcg tgcccggctc
     6121 gacatccgca ggaagtacca ggatgcctgc atgctcctca ccaagtccga tctcgtcttc
     6181 ggcgcagatc atgccatgac tttccacgcc cctgatctta gccaccttca gcgtcagggg
     6241 ctcgccttta gtcggataaa tggttactcc cggcaaagcc accacgacct tttgacccgc
     6301 cccgacgttg gaagccccgc aaacgatcga taaggactct ttcccgccta tacctacctt
     6361 cgttaaacgg agcttatccg cattgggatg aggtccacat tccagtactt ctccgatgac
     6421 caaccccttc aatccccccc tcaccgactc gtatctttcc accccctcta cttccaatcc
     6481 aatggaagtc aatattttag aaagtttttc gggttctatc gcaaccggta gataatcgca
     6541 aagccaattg tagctgatgg tcattgatct gcttttggcg caaaggtaaa ttttttgccc
     6601 gtccctgctt cccgaacttt ccgttcaaca ccccgcctcc ggtaaggccg gcggggaaat
     6661 aatcggcaac tttccgttca acacgccgcc tccggtaagg ccggcgggga aataatcggt
     6721 aactttccgt tcaacagccc ctccggcacg gccgacgggg aaataatcga tcgcgaaatt
     6781 cgttagaatt gtgttaatac ctttgttttg aatttactag gtattcgttt tgaagtccaa
     6841 tacctaactc aaaaactgat atatgaaaaa acgcctactc tacctgtgca tcggtgcgct
     6901 cgccctgcat gcgtgcggac ccgtttacgt ggtccggcaa cccactcctc agcctgctcc
     6961 tgcgacaccg ccgcccgcag acccggagga cgtctcctat cagacctttt acgatcagct
     7021 gagcccttac ggccaatggg tcgaagatcc cgaatatggc tatgtctgga tgcccagcgt
     7081 gggggaggat ttcaaaccct atgcttccaa cggacattgg gtatacaccg atgaaggatg
     7141 gacatgggat tccggctacc cctggggatg ggccgccttc cactatggcc gctggttctt
     7201 caaagacgga tacggatggg aatggattcc cggtaatgaa tgggcgcccg cctgggtcgc
     7261 ctggcgtaat agcgatgact attatggatg ggcccccctg ggaccttcgg tcagcctgac
     7321 cgttgcctat gacggcggct acaatcctcc gccgcattac tggtgtttcg taccgcatca
     7381 atacgtctcc agcccgcagc tcggcaatta ttatgtgcgg gaaacgaaca acgtcacgat
     7441 catcaatcgt accacgatca tccgcaacac gacggtgatc aacaataatc gcaatcgtca
     7501 ttatggttat ggtccggatg cgggcgaggt aggtcgttat tccggttcgc aggtgcaccc
     7561 gatatccatc cacgagatac ggacacccgg cggacaggcc accaatggaa gttttgcggt
     7621 ctatcgtccg catgtcaatt cggcccccgc cggcaatggc aatcagggaa ataatggacg
     7681 gcctgcatat gcgccgactc attatcagtc gatcaataac gttcggccgg tcaacaggac
     7741 caattacggg aatcccggcg gacagggcaa ctccggttcc gaagccggaa cccggaataa
     7801 taatggcaat aatggcaatc acaacggctg gaataacaat aacggcaata atggcaacgg
     7861 gaacaatggc aacgggaaca atggcaacgg gaacaatggc aacggcaatg taaacaacag
     7921 caatggcaac aatggcaacg gcaataacgg gaatcacaac ggctggaata acaataacgg
     7981 caacaatggc aacgggaaca atggcaacgg caatgtaaac aacagtaacg gcaacaatgg
     8041 caatggcaac aatggcaacg ggaacaatgg caatggcaac gggaacaatg gcaacggcaa
     8101 cggaaataat ggcaatggca acggcaacaa cggtaaccac aacggctgga ataaccgctc
     8161 cggcaacccg acccagtcta acacgccacc cgccaatact ccagcggtaa acacgccccc
     8221 tgccaatacc cctccggcaa acaatccccc tgccaatacg cagcaaggca gacctacgac
     8281 gtttcccgga gcgccttccc gccagacgaa taaccccaat aatggtcagc cacaacacgt
     8341 atttcgttcc aatccgaccc tgacccagca acagtcgaca tccggaagac aaccgcaggg
     8401 acgtccaaat gcgcaacccg gaaattccaa ccagtcgaga ccggtaggca acagcgtcaa
     8461 acccgcgcca caacagcaac gcccgccaca ccctcccggc cggccggact ccaaacccga
     8521 caataagtcc taagggacag catttcagcg gaccgggttt tcgctccata tcccggaata
     8581 ccgcactaca gctcattctg gccggcagac taaccctctg ccggcctttt aaaacccgtc
     8641 aaacctcatt ctgtagtttc accttcccgg caaaattttt gcgatcaaat acttgtgcac
     8701 aagaggtgtg aatattaatc tcgaaaggat tgaaaacttt tttaaattgt ggataggttg
     8761 cggaatactg tgcataagcc ccgatttcct gtgaataacc aagtggattt ctaaaggata
     8821 aagaaacaaa aagaaaatta ttgtccgggg gtttcaggca gtctatattc gcctccctga
     8881 cgaaacgggt aacattccct tcatgttgtc gtaacaattc ggtaatgatt gccagctgag
     8941 tcaagccaaa atcctcatat tttttaaaag tattattccc gttggttcgc tatggatcta
     9001 actgaccttc atctaaacaa agacaggaca aaaatgaggc gcaagcctcc cgttgacctc
     9061 agtaccatgg tttacggtaa gattccgccc caggctaaag agctggaaga agccgtactc
     9121 ggcgctataa tgctggaaac cagcgcgttc gataccgtcg tcgagatcct gaaaccggag
     9181 tgtttctacc tcgagggaca ccagcgtatc taccgcgcca tgcagggcct ggcgcagaaa
     9241 agccagccca tcgatatcct gacagtcgtg gaagagctca ggacgcgatc cgagctggat
     9301 atcgtaggtg gtccgtacta cgtgaccaag cttaccaatt ctgtcgtttc taccgccaac
     9361 atcgaaacgc actcgcgtat catcctgcag aaattcatcc agcgcgagct catccgtatc
     9421 agcggcgaga tcatcgggga ggcttatgaa gacagcaccg acgttttcga tctgctcgat
     9481 gacgcggaaa gcaaactttt cgagatcacc aacaaccacc ttcgtaagaa tttcgacagc
     9541 atcgacaccg tactggtcaa gaccattcaa cgtatcgaag acctgcgtca cagacaggac
     9601 gagatcaccg gcgtgcccag cggcttcgcc agtctcgata aggtgaccta cggatggcag
     9661 agtaccgacc tgatcatcct cgccgcgaga cccgccgtcg gtaagacggc ttttgcgctc
     9721 aatctcgcac gcaacgcggc gctccatccg accaaaccga cacccgtggc atttttcagc
     9781 ctggaaatga gctccgccca gctggttcag cggatcctga gtgccgaatc ggagattttc
     9841 atggaaaaga tcgcccgggg ccggctcgaa gagcatgaga tgaaacagct gtacaaaaag
     9901 gggatcgacc ggctggccaa ggcgcctatc ttcatcgacg atacgcccgc attgaatatc
     9961 ttcgagctgc gcgccaaatg ccgccgtctg aagaacaaac atcatgtcgg ctgcatcatc
    10021 atcgattatt tgcagctgat gagtggcgcc ggggataaca aaaatggcaa ccgcgaacag
    10081 gagatcagcc gcatctcccg cgacctgaag ggtctggcca aggaactaca ggtgcccatc
    10141 atcgcccttt cccagctgag ccgcgcggtc gaatcccgca aagaaggaga gaagatacca
    10201 cagctgagcg atctgcgtga atccggcgcc atcgagcagg atgcggatat ggtcatgttc
    10261 ctctatcgcc ccgaatatta cggcatcacc gctaatgaga atggcgaaag caataagggc
    10321 gagacccatg tcaagatcgc caagcatcgt aatggctcac tggagacgat caagctgcgc
    10381 gccctgcttc acatccagaa attcactgaa tacggtgacg acgatttcgg cggcatcggc
    10441 atgccaggcg gcggcaactg gaagccagtc caggacaatg aaggcggagg cgcccggctc
    10501 tatatccagg ccggcagccg gatgaaggat ctgcccgtgg atgacgatga gacgccattc
    10561 tgattctatg gccttgccct tcttttatat taccgcttat gttgctgacc aacaggaact
    10621 tatattggat gagggcaatt cgcggcacgt tgtccaggtg ctccggatga aacccggaga
    10681 atcgctgcgt cttacggacg gcaaaggcgc cctgctcacg gcggagatca tcgacgatca
    10741 caaaaagaaa tgccgtgttt ccatcctgac ggccaccttc cagcctccgc ccgaaagaaa
    10801 agtgaccatt gccatctccc cgctcaaaaa tgcgtcccgt ttcgaatggt tcctggaaaa
    10861 agcgaccgaa ataggagtca gcgccgtcat tcccctcgtc tgcgaacgta ccgaacgtcc
    10921 ctccatccgg cacgaccgtc ttcagcagat tgcggtcagc gcccttctcc agtcgcaaca
    10981 ggcctggctg cccgatctgg cggctccgat ccccctggcc gatctcctgc caaattccac
    11041 catccgaaat cgctacatcg cacactgtct cgaagccccg tgtccgacag tcagtctcac
    11101 cgacactccc caagcggacc attccctcat cctcatcgga ccggagggag acttttccat
    11161 aaaagaaatt gaaaatgcac tggcggccgg cttcatcccc gtcacccttg gtcccaaccg
    11221 gcttcgcacc gaaacagccg gcgtcgtcgc cgctacctta ctttgtatag cgtaatctca
    11281 tttttggtat ttccacatcc tcgacttgcc ctctaccagc cattcgatcg tagtggcgac
    11341 acgcttctct ttcgtcgcat ccgtctttgc ctctacgatc cattcgatat actccctgcg
    11401 atgcgagggt gaaaaaccct cgaatgcggc tttcgccgct ttgttttttt tcagcgccgc
    11461 ggcgagctcc ggcggtaccg gtaattccgg cttaggcgct ttttttgcca ccggtttctt
    11521 tacccccttc tcattcagcg aagcaccttc tttcagatac gcgatgatga tcttatctgc
    11581 cggcagatcc ttcagcgagg tgatccggtc gaaatgcccc atggcattct tgtccttcac
    11641 ctgaaggatg ccctccgggt ctttcagtaa aggtgcattc catagtccga acgaacaatg
    11701 ctctttgaaa gcggcaatat tgcagaggat cgaaccgctg gtctcgaaga atggcatccc
    11761 ccatttgata gtttcggtag catcaggaca ggccttatgg acgagcttgc gcaggtgatt
    11821 cagaatgggt ttggcaaaat cggcggattt ggcgatatag gcgtcgatac gtgcatcggt
    11881 cgcgggcata tgtagcagtt tttaggaaga tgaatatagc ttattttttt cttacccggc
    11941 tattcagcag ctcgaccccg aaggcaaacg ccatcgagaa atagatatac cccttgtcga
    12001 tatgcatgtg atgcaacggc tcgagtccct cgaaaagaag actgaaaccg atcatcagca
    12061 aaaaggataa ggccaggatc ttgacggtag gtcgccggtt gataaaattc gcgatgcttc
    12121 gcgaaaaaaa gaacatcagg atcatggcca ggaccaccgc cgtgatcatg acggcgatct
    12181 gctgcgccag ccccaccgcc gtgacgatgc tatcgaagga aaagaccgta tccaccagca
    12241 cgagctgagc gaccagtccc caaaacgaag ggttctcttc atccgtcgcc tcctcgcctt
    12301 cctgcgggtc gccctccagc ttctcatgga tctccctgac ggtcttatat aagaggaata
    12361 accctcccgc ggtcatgatg aggttgcggg tgctgaaccc gtaccccgcc agagagaaca
    12421 atttcgtgtt accgctggtg acaagccagc ccagccccag gaggagaccg ctgcggacca
    12481 ggctgccggc gaccatccat acacgtcggg ccgccagctg ttgatgagaa ggaaggcgtc
    12541 cgatgatgat cgagacgaag atcacattgt cgatccccag gacgatctcc agtacgataa
    12601 gcgtgacgag actcaccagc gagtcccagc tgaacaattg atgcatgcac ggtcggttaa
    12661 gccgcacaaa aataggggat tcaccggcat atcctcctca ccacgaacgg cccctcataa
    12721 ataaaccctg tccacacctg caccagcacg gcgcccgcgc cctgcttctc gcgcatatcc
    12781 gccaccgtga atatcccgcc cgacgcgatc accggtatcc gcccgcccaa ccgggcacaa
    12841 cagtatcgta ccacctccgt ggacagctcg cggaccggcg ccccgctcaa gccccccgcg
    12901 ccaatttcgg caacgcgccc ggcagacgtc aatagtccgt cccggcttat cgtagtattc
    12961 gtagccacca atccgtccag gccgatctcc actgccagat cgacaacgtc atccaattga
    13021 gaagaagaaa gatccggcgc gatcttcaat aacaagggcc gtggccgaac gcgcaagacc
    13081 cccgacatct cgcgattcac accctgcaaa ttgaccaaaa tcttccgtaa cgaatccttc
    13141 tcctgcaact cccttaatcc cggcgtattc ggtgagctca catttaccac gaaataatcc
    13201 accgcgtcgt acaatgcgcg aaaacagatc tcatagtcct tccacgcgtc ctcattcggc
    13261 gtgaccttat tcttcccgat attaccgccg acgatcaacc ccgaatttcg gcccgcccgc
    13321 gaagacctcc aggcgcggac ccgcgccgcg accgcctcca ctccgtcatt attgaaaccc
    13381 atccggttga tcaacccctg gtcctccggc aaccggaaca gccgcggccg cggattccct
    13441 tcctgcaccc ggggcgtcac cgtcccgatc tccacgaacc cgaaccccaa cgcgtccagc
    13501 tcgcgcagat acaacgcatt cttatcgaaa cc
//
LOCUS       DASYSV010000226         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2346092, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000226 DASYSV010000000
VERSION     DASYSV010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2346092"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..709
                     /locus_tag="VGQ51_07035"
     CDS             98..709
                     /locus_tag="VGQ51_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9036353.1"
                     /translation="MSNDGQDITLWESFRNGDKEAFSLLFRRHYESLVRYGSKFTANT
                     EVLEDSIQELFIELWQAKSRTPVLSVRAYLLKALKYKLLKKYDRMQDTVPLKEDEGGF
                     EWSPESSLIARQDSDEKEKIVTVALNGLSHRQKEIIYLKYYQNLSYEEVSDIMNINYQ
                     AARNLLYQAIKALRSRLPGSLELIFFLFAGSQHYIFHGFGTFC"
     gene            782..2689
                     /gene="nagB"
                     /locus_tag="VGQ51_07040"
     CDS             782..2689
                     /gene="nagB"
                     /locus_tag="VGQ51_07040"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV9036354.1"
                     /translation="MGMVDSFEKIPVKIFSSLEEGSRFIGKEIAAIIRDKESRGEKAV
                     LGLATGASPKSLYAELVRLHRDEGLSFRNVITFNLDEYYPIDNDALQSYNRFMKEHLF
                     DKVDIDPANCHIPNGQWKKEEIKQHCIQYEQMIEKAGGVDLQILGIGNNGHIGFNEPG
                     SSIYSHTRLVPLENSTRIANSHDFQNISQVPRLAITMGISTILKAKRIVLMAWGQLKA
                     PVIRKAVEGNVTEQIPASLLQQHNDVVFVVDDIAASELTRFKSPWLTGDMVWTPAMMR
                     KAVVNMALRLEKPILSLTNSDYNDNGLSDLLVEKGDAYEINLQVFYMLRDSITGWPGG
                     KPAANLPSHPERSEPFPKRCLIFSPHPDDDIISMGGTFMRLQDQGHDVHVAYQTSGNI
                     AVTDEFVTRFLDFAVGFEVLSDIDSKKSSQILEQATNYLRTKKASDIDTAEIRAIKGL
                     IRRCEAKATCRYVGIPGTNIHFMNLPFYETGTIEKKPMGEMDVELTVGLLRKLKPQQI
                     YCAGDLADPHGTHKVCLDVVFESLRRLKAAGDPWLKDCWLWLYKGAWQEWDISEIEMA
                     IPMSPDQVLKKRFGIFIHQSQKDMVPFQGNDSREFWQRAEDRNANTAHLYAQLGFTKY
                     AAMEAFVRWHY"
     gene            2876..>3349
                     /locus_tag="VGQ51_07045"
     CDS             2876..>3349
                     /locus_tag="VGQ51_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036355.1"
                     /translation="MSQSTFIEVKQEWQGAWRVPGFRSKMIIGVILVLATLSCFPIFF
                     QWIEHRPGTPLADPILRWLPARNVSVPLFILIWSLSLLTLVRAIQTPRILLVFLWSYF
                     LLSLLRMLTIYLLPLEPPANLIGLVDPLSNFFYGPKFITKDLFPSGHTSTMFLLFL"
BASE COUNT          791 a          930 c          852 g          776 t
ORIGIN      
        1 ttccgttttt ttagcgattt tcatcaaata ttttacccga aaataatatt gctgtatttt
       61 cctcctttga tttgcatatc acaaccataa ctatctcttg agtaatgacg gccaggacat
      121 aacgctttgg gaatcctttc gcaacggcga caaagaagct ttctcgctgt tgttcagacg
      181 gcattacgaa tccctggtca gatacggcag caagttcacg gcgaatacgg aggtgttgga
      241 ggacagcatc caggaactgt tcatcgagct ctggcaggcc aaatcccgga cgccggtgct
      301 ctcggtccgc gcgtatctcc tcaaggcact gaaatacaag ttgctaaaga aatatgatcg
      361 aatgcaggac accgttcctt tgaaggaaga cgaaggcggt ttcgaatgga gtcccgagtc
      421 ttcgctcatt gcccggcagg acagcgacga aaaagaaaag atcgtcacgg tcgcgctgaa
      481 cggtctgtcc caccggcaga aagagatcat ctacctgaaa tactaccaga acctcagcta
      541 cgaagaggtc agcgatatca tgaatatcaa ttatcaggcg gcacgcaatc tgctttacca
      601 ggccataaag gcgctcagaa gcaggctgcc gggctcgctc gaattgattt ttttcctttt
      661 tgcgggttcg caacattaca tttttcacgg tttcggcacc ttttgttgac ggcgtccgaa
      721 ggatgcttta ttttcgcggc gcgacaccac tacattcaag acaaactaac tttttcagac
      781 catgggcatg gtagactcat tcgagaagat acccgtaaag attttcagtt cgttggaaga
      841 aggctcccgt ttcattggaa aagaaattgc ggcgatcatc cgtgacaagg aaagccgcgg
      901 cgaaaaagcg gtcctcggac tggcgacggg cgcctccccc aaatccctgt atgccgagct
      961 ggtgcgtctg caccgcgacg aaggcctcag cttccgcaat gtcatcacct tcaatctcga
     1021 tgaatactat cccatcgaca acgatgcgct gcagagctac aaccgtttca tgaaggaaca
     1081 cctcttcgat aaggtggata tcgatcccgc caattgtcat atccctaatg gacagtggaa
     1141 aaaagaagag atcaaacagc attgcatcca atacgagcag atgatcgaaa aggcgggcgg
     1201 cgtcgatctc cagatcctgg gtatcggtaa caacggtcac atcggcttca acgaaccggg
     1261 ttccagcatc tattcgcata cccggctggt gcccctggag aattccaccc gcatcgccaa
     1321 ctcgcacgat tttcagaata tttcacaggt gcccagactc gccattacca tgggcatcag
     1381 caccatcctg aaggccaaac gcattgtgct catggcctgg ggccagctca aggcgcccgt
     1441 gatccggaag gcggtggagg gcaacgtcac ggaacaaata ccggcttcat tgcttcagca
     1501 acataacgat gtcgtattcg tggtcgatga cattgcggcc tccgagctca cgcgtttcaa
     1561 atctccctgg ctcacagggg atatggtgtg gacacctgcc atgatgcgca aggccgtggt
     1621 caatatggcg ctccggttgg aaaagccgat cctgagtctt accaacagcg attataatga
     1681 caatggtctc agcgatctgc tggtggagaa aggagatgcg tacgagatca acctgcaggt
     1741 attctatatg ctgcgcgata gcatcaccgg ctggccgggc ggcaagcctg cggcaaatct
     1801 tccatcgcac cccgaacggt ccgaaccttt tcccaaacgc tgtctgatct tctcccctca
     1861 cccggacgat gacatcatct cgatgggagg cacgttcatg cggctgcaag accagggaca
     1921 tgacgtacac gtggcttacc agacatcggg aaacatcgcc gtgaccgatg agttcgtaac
     1981 ccggttcctc gatttcgcgg tcggtttcga agtgttgtcg gacatcgaca gtaagaaatc
     2041 gtcacagata ctcgaacagg ccaccaatta tctccggacc aaaaaggcca gcgatatcga
     2101 taccgcggag atccgggcca tcaaggggct gatccgccga tgcgaggcca aggccacctg
     2161 tcgttatgtg ggcatccccg gcacgaacat ccatttcatg aatctgccgt tctatgagac
     2221 gggcaccatc gagaaaaagc cgatgggcga aatggatgtc gaactgacgg tcgggctgct
     2281 gcgcaagctc aaaccacagc agatctattg cgccggcgat ctggccgatc cgcacggtac
     2341 gcataaagta tgtctcgatg tcgtctttga atcgctgcgc cggctcaagg ccgcgggcga
     2401 cccctggctt aaagactgct ggctctggtt gtacaagggc gcctggcagg aatgggacat
     2461 cagcgagatc gaaatggcca ttcccatgag ccccgatcag gtcctcaaga aaagattcgg
     2521 catcttcatc caccaatcgc aaaaggatat ggtgcccttc cagggtaacg atagccgcga
     2581 attctggcaa cgggctgaag accgcaatgc caataccgcc catctctacg cgcagctggg
     2641 gttcacgaag tatgccgcca tggaggcatt cgtgaggtgg cattactgag tcgccacagc
     2701 gcctgctggg ccgaaaggcc cgggggcctt tcgcgaaccg aaggttcgca gacattagcc
     2761 ccggttaggc gtcgcttgcg acgttgcgga gcaacaagtt caataataaa tcctgattcg
     2821 ttcctgtatt atgtttgtgt taacccgcgc ttttggctaa atttgccggg taggtatgtc
     2881 tcaatcgact ttcattgaag taaagcagga atggcaggga gcctggaggg ttccgggttt
     2941 tcgttccaag atgatcatcg gagttatcct tgtcctggca actttatctt gttttcctat
     3001 ctttttccag tggatcgaac atcgtcccgg tacccccctc gcagatccta tcctgcgctg
     3061 gttgcccgcg cgtaacgtct ccgttccgct ttttatcctg atctggagcc tgtccctgct
     3121 gacgctcgtc cgcgccattc agaccccccg gatcctgctc gtatttctct ggtcttattt
     3181 tttattgagc ctcctccgga tgctgaccat ctacctgctg ccactggagc cccccgccaa
     3241 cctgataggt ctggtcgacc cgctgagcaa tttcttttat ggaccgaaat tcataacgaa
     3301 ggacctgttc ccgtccggcc atacgtcgac catgttcctg ctcttcctc
//
LOCUS       DASYSV010000227         7002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1962847, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000227 DASYSV010000000
VERSION     DASYSV010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962847"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGQ51_07050"
     CDS             <1..273
                     /locus_tag="VGQ51_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEV9036356.1"
                     /translation="LDRQVYHLYIRYLGKETIRTAYGKFRTIKFKPLLVKGTMFQGGE
                     KMTVWVTDDPNHIAVRVESPITVGKVRVDMIMYHNPRYPLTSLVDL"
     gene            634..1131
                     /locus_tag="VGQ51_07055"
     CDS             634..1131
                     /locus_tag="VGQ51_07055"
                     /inference="COORDINATES: protein motif:HMM:NF039195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HEV9036357.1"
                     /translation="MYSFIHVKSLFLLLIPGWLTYTSQTGKFSMQYPKEWSPKAQNNA
                     ILFLSPKADAQDQFQENVNIILQDLSSQPMTLEQYTDMSKQQLIQAFGAKAVLSQGAT
                     TIAGQKAQYMVYSFTYQGRSLKIKGVWFIKGKTAYLLTYTAEPSQYAKYEQTATAIIN
                     SFSFN"
     gene            1142..1492
                     /locus_tag="VGQ51_07060"
     CDS             1142..1492
                     /locus_tag="VGQ51_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV9036358.1"
                     /translation="MRKWTSLVFSLTIASALPGCNFPTGKYTIHVVFDDLESANVQTQ
                     VRLYGDEVGEIQKCILLQPHQLLVDLTISKDVRIDRCDSIIYHETLIGDPYIQLARGR
                     RLSVPGRQANDSTR"
     gene            1543..2694
                     /gene="glf"
                     /locus_tag="VGQ51_07065"
     CDS             1543..2694
                     /gene="glf"
                     /locus_tag="VGQ51_07065"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEV9036359.1"
                     /translation="MDLPDYSYVVVGSGLLGAVIAERIATQLDQPVLVIEKRPHIGGN
                     CYSATDPTTGIEYHQYGTHIFHTSNEKVWNYVKKFTSFNNYRHKVLTSHDGRTYPFPI
                     NLATINQFYNKNLKPFEVEPFMATIREPIPDPENFEEQALSRLGRPLYEAFYKHYTIK
                     QWQTDPKNLPAAIFNRIPFRTSYDDNYFFDRWQGIPADGYTGLFQRLLANKKIKVLLN
                     TDYFDLRDRLNKHACLIYSGPIDRFFDYKYGKLSWRTLRFEHQTLETGDYQGNSVINF
                     PDAADPYTRIHEPRHLHPEKAYPKDKTIIFREYSLPDQGDNPYYPIANEANQQLLAKY
                     RHEAAGLKNVFISGRLGDYKYYDMHHTIDRALEIFEMGILPLTHANHAV"
     gene            2684..3427
                     /locus_tag="VGQ51_07070"
     CDS             2684..3427
                     /locus_tag="VGQ51_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9036360.1"
                     /translation="MPYNPDQAAVDPQPIPQKTELAIVIPVYNEEQNLRALFRDWQPV
                     FKASGASYRIIAIDDGSADDSVRLLQSLQSENPVISIHTQPNSGHGAAILKGYRLALD
                     AEWVFQMDSDHQLDTGAFVRLWANRNDYDLLIAQRADRNASKGRRWISRFSTGMVRML
                     YGRGVTDVNSPYRLMRSQVLRSALEKIPAGSFAPNVLLTSWFILKKRRIFTTTVELRN
                     DGLRRSRLNRYFLRGSFRSCIQTILFRIR"
     gene            3424..4911
                     /locus_tag="VGQ51_07075"
     CDS             3424..4911
                     /locus_tag="VGQ51_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036361.1"
                     /translation="MTRKHKYLLFIILGISFSLSFCFSPPYDLMRDDREVFSYAGMAI
                     LKGQVPYRDFFDHKPPGIFFINAAGSLLGGLWGLWAINTLLVLIATWMLYRICLQYRL
                     AFSWLLPLLFNLMIRDNLISMGASMTREFTTIFFLFFFCIFMGRSRYREMSLGLCTGL
                     IFFTQQEQVLATVPFLLYVLAAERSLPINQRIARMALGFGAVLVPILLYFAWNRSLGY
                     LWDDGFRFNMIWYTAQPKSPGDHFRTIKRCLDAGNYEMPFMIALILGVASFFTKHKKK
                     GLLLTALAAFFLSLAPELMGNRFEGRANPIDSPPYFLPLAASICALLFVIFAFSDEAI
                     IGDKKIQLPFALLLLCSLTYTSLQHSTHLEKRSDDSDINSPALNYLRQHRPGNYDLYV
                     FNDDDYIYAYNEFGILAPSRWIYHHMWDWYYRWDADHALLSGITADLLRHHTTYIIMD
                     PVRMTQFLNPANTAYWRDFMEKNYEPQPVPGTRILWKRKETPPNR"
     gene            4940..6409
                     /locus_tag="VGQ51_07080"
     CDS             4940..6409
                     /locus_tag="VGQ51_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036362.1"
                     /translation="MLLLILTASLLLSLLYIPKYNEISGDKEIYRYVGLLMHKGGVPY
                     RDAFDHKPPLIFFLNYAGVLLNGWTQWLIDAGLVLLATFLFFRLGEKYRLPFPWVPPL
                     IFNLMIRDHLLCLGIGMTREYTTVLMVIFFCVLMGKHRFRYFGLGAIGASIFFMQQDQ
                     VLQLIPFFLYSFLPATDSLPFGRRLLRVFLGFASVTLPLILYFAWNHALTVAWQDAFL
                     FNFGWYTHTLKESFRDHLIKLKTLMDGGNYEVAVLVTVVLGMCASIFPSSNRKLILLA
                     LLAIPLSVAAEFMGGRDIVPNIYRITFTHYILPLAGSIPILLFCAFAFTREPVLSGYR
                     AHAIFSIIAALSLAYTAAQHATHLKSKDVDEVAAAPELPYLLQQRLSDYQFYVFGSIN
                     SVYMTNELNVRAPSVWVYQHFWRLYPTWDTDLRLLRSIEQDLLRHRTTYVLDLTSGGD
                     WFSNCAADEEWHGFLRDNYAPVMVNSVLESTLWRKKEVR"
     gene            complement(6406..>7002)
                     /locus_tag="VGQ51_07085"
     CDS             complement(6406..>7002)
                     /locus_tag="VGQ51_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV9036363.1"
                     /translation="THLHFDHCGGSITREADKLVPAFKNAHYWSHSRHWKWATEPNDR
                     EKASFLKENILPIQESGRLRFVDQEYAADQKTGADCGGAGEPGRLRDVILPGISVRLA
                     EGHTAAMMLPQLSYRGRTVVFMADLLPSMGHFPQPYVMAYDMFPLTTMQEKKAFLEDA
                     AAGGYVLFFEHDPGTECCIPERTDKGVRPGKGFRLAEL"
BASE COUNT         1633 a         2103 c         1568 g         1698 t
ORIGIN      
        1 ctcgatcgtc aggtctatca tctttatatc cgctatctgg gcaaagagac catccgaacc
       61 gcatatggca aattccgcac catcaaattc aaaccgctgc tcgtcaaggg aaccatgttc
      121 cagggtggcg aaaaaatgac ggtctgggtc accgatgatc cgaaccacat cgccgtccgc
      181 gtcgagagcc ctatcaccgt cggcaaggtc cgcgttgaca tgatcatgta tcataatccg
      241 cgctatccac tcacctcgct cgtcgatctg taatttcccg ctgcactcaa attgcagcca
      301 ttttgcacgc atctcttcag aaaacctcag aaatattctt atattgttga atgcgaaatg
      361 tacattgacc cgctggaggt cagcataacc gacctcgctg gtaacctcgg agttgcccgt
      421 aaaacggtat cacccccgag ttttggttga acatgcaacg aaactttgat ttgtggcgcg
      481 ccggaacaaa aataaacgta acgcatattc gatcgctccg gacaaaagag cgcagaacgc
      541 aaaaggccag ttagtggatt ttatctcaag tcccccattt gcgccgtccg caaaaccttt
      601 gtgcttcacc gttacataaa cactaagatc aacatgtact cctttatcca cgttaaatcc
      661 ctgttcctgc tgcttatccc cggctggctc acctatacca gccagaccgg caaattctcg
      721 atgcaatatc caaaggaatg gtccccaaag gctcaaaaca atgccatcct tttcctctcc
      781 cccaaggcgg atgcccagga tcaattccag gaaaacgtca acatcatact gcaggacctt
      841 tcctcccaac ccatgaccct ggaacaatat acggatatga gcaaacaaca gcttatccag
      901 gccttcggcg ccaaggctgt cctctcacag ggtgccacga ctatcgccgg gcaaaaagcc
      961 caatacatgg tttatagctt cacttaccag ggccgttccc tgaaaataaa aggcgtttgg
     1021 ttcatcaaag gaaaaacagc atatctctta acctacactg ccgagccttc gcaatatgcc
     1081 aaatatgagc aaacggccac ggctatcatc aacagctttt cctttaacta gcaacccgct
     1141 tatgcgtaaa tggacatccc tcgtcttttc tttaaccatt gcttcagcac tgccgggatg
     1201 caactttccc accggcaaat acacaatcca cgttgttttt gacgacctcg aatctgccaa
     1261 tgtacagact caggtccggc tctatggaga tgaggtcggc gagatacaaa aatgcatcct
     1321 tctccaacca caccagctat tggtggacct gacgatctca aaggatgtcc ggatagaccg
     1381 gtgcgacagc atcatttatc atgaaaccct tatcggtgac ccctacatcc agctagcccg
     1441 tggtcgccgt ctcagcgtcc ccggccgtca ggccaacgac agcacacgct aaaaagacgg
     1501 caattccctt catgacattc ccgctaaacc ttatcttgcg ccatggacct ccctgactac
     1561 tcctatgtcg tcgttggcag cggcctcctg ggcgccgtca tcgcggaacg catcgccacg
     1621 caactggacc aaccggtcct cgtcatcgaa aaacgccccc acatcggcgg caactgctat
     1681 tcggcgaccg accccaccac cggcatcgaa taccaccaat acggcaccca tatcttccat
     1741 acgtccaacg aaaaggtatg gaactatgtc aaaaaattca cctcgttcaa caactaccgc
     1801 cataaggtcc tgacctccca cgacggccgg acatacccgt ttcccatcaa cctcgccacg
     1861 atcaaccagt tctataacaa aaacctgaaa cccttcgagg tcgaaccctt catggcgacc
     1921 atccgcgaac ccatccccga tcccgaaaat ttcgaggaac aagccctcag ccgtctcggc
     1981 cgcccgctgt acgaggcatt ttataaacac tataccatca aacaatggca gaccgatcca
     2041 aaaaacctgc cggcagccat cttcaaccgg atccccttcc gcaccagcta cgacgacaac
     2101 tattttttcg atcgctggca gggcatccct gccgacgggt acaccggcct atttcaacgc
     2161 ctgctggcta acaaaaagat aaaggtcctt ctcaataccg actatttcga tctccgcgac
     2221 cggctgaata aacacgcctg tctcatctat agcggcccga tcgacagatt cttcgactat
     2281 aaatacggga agctcagctg gcgcaccctg cgcttcgagc atcagaccct cgaaacgggc
     2341 gactaccagg gtaattccgt catcaatttc cccgatgccg ccgaccccta tacgcgaatc
     2401 catgagcctc ggcatcttca ccccgaaaag gcgtacccga aagataagac catcatcttc
     2461 cgcgaatatt ccctgcccga tcagggtgac aacccctatt atcccatcgc caatgaggcc
     2521 aaccagcagc tgctcgcaaa ataccggcat gaggccgcgg gacttaagaa cgtctttatc
     2581 agcggacgcc tcggcgatta caaatattac gatatgcatc acaccatcga tagggccctc
     2641 gagatcttcg aaatgggcat cctgccgcta acccatgcca accatgccgt ataacccaga
     2701 tcaggcggcc gtggaccctc aacccatccc acagaaaact gaattggcga tcgtcatacc
     2761 ggtctataat gaggagcaaa atctccgcgc ccttttccgc gactggcaac ccgtcttcaa
     2821 agccagcggc gccagttacc ggatcatcgc catcgacgac ggttccgccg acgacagtgt
     2881 aagattgctg caatcccttc aatcagaaaa ccccgttatt tccatccata ctcagccaaa
     2941 ttcgggtcac ggggccgcga tccttaaagg ataccggttg gcgctcgacg ccgaatgggt
     3001 atttcagatg gactccgatc atcagctgga taccggggcc ttcgttcggt tgtgggcaaa
     3061 tcgcaacgac tacgatctgc tgatcgcgca gcgcgcagac aggaatgctt cgaaaggccg
     3121 ccggtggata agccggtttt cgactggcat ggtccgcatg ctctatggcc gtggggtgac
     3181 ggatgtgaat agcccctatc gtcttatgcg atcgcaagta ttgcgatccg cacttgaaaa
     3241 gataccagcg gggagttttg caccaaacgt cctgctcacg tcctggttta tcctaaaaaa
     3301 aagaaggata tttaccacga cggtcgaatt gcggaatgac ggtcttcgga ggagccggct
     3361 caaccgttat tttctgcgtg gatcctttcg ctcctgtatt cagaccattt tattccggat
     3421 aagatgacga gaaaacacaa atatctcctt ttcatcatcc tcggcatttc cttttcgctg
     3481 agcttttgct ttagcccgcc ttatgacctg atgcgcgacg accgtgaggt attcagctat
     3541 gcaggcatgg cgatcctcaa agggcaggtc ccctatcgcg attttttcga tcataagcct
     3601 cccggaatct ttttcatcaa tgccgcgggt tctcttctcg gcgggctttg gggcctttgg
     3661 gcgatcaata ccctactcgt cctgattgcg acatggatgc tttaccggat atgcctgcaa
     3721 taccggctgg ctttttcctg gctccttccg cttttgttca atctcatgat ccgggacaac
     3781 ctgatcagta tgggcgccag catgacgagg gaatttacca cgatcttttt tctcttcttt
     3841 ttttgcatat tcatgggcag atcgcggtat cgcgagatgt cgctgggtct gtgtaccgga
     3901 ctgattttct ttacccagca ggagcaggtc ctggcgaccg tgccgttcct gctttatgtc
     3961 ctggcggcag aacgatccct gccaatcaac caacgcatcg cccggatggc cctcggcttt
     4021 ggcgcggtcc tggttccgat cctcctttat ttcgcatgga accgttcgct tggttattta
     4081 tgggatgacg gcttccggtt caacatgatc tggtatacgg cgcaacccaa atcgcccggt
     4141 gaccatttcc ggaccatcaa gcgatgcctg gatgcgggca actatgagat gccattcatg
     4201 atcgcactta tcctgggcgt cgcttctttc ttcaccaaac acaaaaagaa aggcctgctc
     4261 ctcaccgccc tcgcggcttt ttttctctcg ctcgccccgg aattgatggg caatcgattc
     4321 gaaggtaggg caaacccgat agactctccc ccgtatttcc tgccgctggc agccagcatt
     4381 tgcgcactcc tgttcgtcat cttcgccttt tcggatgagg cgatcattgg ggacaagaag
     4441 attcagttgc cctttgcctt gctgcttctc tgcagcctga cctatacttc tcttcagcat
     4501 tccacccacc tggagaaacg cagcgacgac agcgatatca actcccccgc actcaactac
     4561 ctgcgacagc accggcccgg caattacgat ctatatgtct tcaatgacga tgactatatt
     4621 tatgcctata acgaattcgg gatcctcgcg ccgtcgcgct ggatctatca tcatatgtgg
     4681 gactggtatt atcgatggga tgcggatcat gcccttctgt ccggcattac cgccgatctg
     4741 ctccgacatc ataccaccta tattataatg gatccggtcc gtatgaccca attcctcaac
     4801 cctgccaata cggcctactg gcgggacttt atggaaaaga attacgaacc gcaacccgtt
     4861 ccgggcacca ggatcctatg gaaacgcaaa gagacccctc caaatcgata agaaagatct
     4921 tatgaaccga caccggatca tgcttctgtt aatcctgacc gcatcgctcc tgctgagcct
     4981 gctttatatt cccaaataca atgagatatc tggtgacaaa gagatctatc gatatgtagg
     5041 attgctgatg cacaaaggtg gagtacccta cagggacgca ttcgatcaca aaccacccct
     5101 gatcttcttt ctcaactatg cgggggtcct gctgaacgga tggacacaat ggctgatcga
     5161 tgccggactg gtcctgctgg caacctttct tttctttcgc ctgggcgaaa aatatcgatt
     5221 gccttttccc tgggttccgc ccctgatatt caacctcatg atcagggacc atctgctatg
     5281 tcttggcata ggcatgactc gtgaatacac gaccgtcctg atggtcattt ttttctgtgt
     5341 cctgatggga aagcatcgtt tccggtattt tggtctcgga gcgatcggcg cctcgatctt
     5401 ttttatgcaa caggatcagg tgctgcagct tattcctttt tttctctaca gttttttgcc
     5461 tgccacggac agtttacctt ttggccgccg gctgcttcgc gtattcctgg gtttcgcctc
     5521 ggtgacgtta ccattgatcc tgtattttgc ctggaatcac gctttgaccg ttgcctggca
     5581 ggatgcattc ctgtttaatt tcggctggta tacccacacc ctgaaggagt ccttcagaga
     5641 ccacctgatc aaactaaaaa ccctgatgga tgggggaaac tacgaggtgg cagtcctggt
     5701 aacggttgtc ctcgggatgt gtgcttcgat cttcccatcg tccaaccgga agctgatcct
     5761 gcttgccctg ttggccattc cgttatccgt tgccgctgaa ttcatgggcg gacgcgatat
     5821 cgttccaaat atataccgca tcacctttac gcattatatc cttcccctgg cgggttccat
     5881 tcctattttg ttgttctgtg cattcgcgtt tacgcgtgag ccggtcttgt cgggttacag
     5941 ggcacacgcg atattttcaa ttatcgcagc cctgagtctc gcatacaccg ccgcccagca
     6001 cgcgactcac ttgaaaagta aagatgttga tgaggtcgcc gccgcgcccg aattacccta
     6061 tcttcttcag caacggctgt cggactatca attctatgta ttcggtagca taaattcagt
     6121 ttatatgacc aatgaactga acgtacgggc cccttctgtc tgggtgtacc agcatttctg
     6181 gagattatac ccgacctggg ataccgacct ccggctgctg cgcagcatcg agcaggacct
     6241 cctgcggcat cggaccactt atgtactcga tcttacttct ggaggggact ggttttcgaa
     6301 ttgcgcggcc gatgaagagt ggcatggttt cctgcgcgac aactatgccc cggtgatggt
     6361 caattcggtg ctcgaatcta cgctatggag aaagaaagaa gtgcgctaga gctctgccag
     6421 cctgaatccc ttgcccggcc gcacaccctt atctgtccgc tccgggatac agcattcggt
     6481 accgggatca tgttcgaaga aaagcacata cccgccggcc gcggcatctt ccaaaaaagc
     6541 ctttttctcc tgcatcgtcg tcagcgggaa catatcataa gccatgacat aaggctgagg
     6601 gaaatgaccc atcgatggca acaggtcggc catgaacacc accgtccgcc ccctatagga
     6661 cagctgcggt aacatcatcg cggccgtatg accttctgcc agacggaccg atatcccggg
     6721 tagtatcacg tcgcgaagcc tccccggctc ccccgcaccc ccgcagtcag cacccgtctt
     6781 ctggtccgcg gcgtactcct gatccacgaa ccgcaaccgc ccactctcct gtatcggcag
     6841 gatattctct ttcaaaaacg acgccttttc ccggtcgttc ggttccgtcg cccatttcca
     6901 atgccgcgaa tgactccagt aatgcgcatt cttaaaggcc ggcaccagtt tgtcggcctc
     6961 ccgggtaata ctacctccgc aatggtcgaa atgaagatgg gt
//
LOCUS       DASYSV010000228         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2472736, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000228 DASYSV010000000
VERSION     DASYSV010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472736"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VGQ51_07090"
     CDS             <1..747
                     /locus_tag="VGQ51_07090"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV9036364.1"
                     /translation="TAYLLNESPDGRVELQKDGQFAFTPKDGFTGNSTQFSYKVCDNG
                     SPALCSITTTAVIRFPSKSTLVSFDALYSRKEVAINWNIATDNHSKTFEVERSLDGSF
                     YKKVGEVKAEEAIMEGKYTFNDKMHEAERRNDLYYRLRQIDGAGRVTYSKVLIMRSYG
                     SKSVEAVSVTPDPGVNDIQVNVQLKEKSFVVMRVTDNNGSELIKQTAMGDLGTNQYNI
                     EGTSQLQPGLYQLEVIINSNERMTMKLAKS"
     gene            complement(788..1078)
                     /locus_tag="VGQ51_07095"
     CDS             complement(788..1078)
                     /locus_tag="VGQ51_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV9036365.1"
                     /translation="MFKDLNPILHSQLRLAVVSILISVKEAEFTYLREKTDATAGNLS
                     VQINKLKEAGYIDVTKSFKDNYPQTICRITPEGIRAFEEYVKDLQTYLNIKK"
     gene            complement(1071..1661)
                     /locus_tag="VGQ51_07100"
     CDS             complement(1071..1661)
                     /locus_tag="VGQ51_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036366.1"
                     /translation="MEEKELTGQESLALITKMINKARRDYLDSGLSALLWGSVITFCS
                     LVTFANYWIKQPVFDYVWFLTIAAVVPQIIIAIREAKKRKHRAYDEDIMGGIWISFGI
                     AIFLLSYITNAYQVNEEGPLFLTLYGVPTFATGYARRFKPMIIGGLACWLLAILCHYT
                     PLPYKILCMTAAAQLAWFIPGLILRKCYLKAKKQHV"
     gene            complement(1711..>2188)
                     /locus_tag="VGQ51_07105"
     CDS             complement(1711..>2188)
                     /locus_tag="VGQ51_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036367.1"
                     /translation="DRIGDAEKTQWLPYYYAALAQTWVGWNPETKDKDGNAAKIKSYL
                     SKAEAIEKNAETYAVENMLSTQQMLMDPQTRWATNGKDASDALQKGMSIDPNNPRLYY
                     LQAMSLFGTPPQFGGGKDKAKPVFEKSIALFKSTQPKPLYPTWGLPQAEQGLAQCQ"
BASE COUNT          497 a          501 c          660 g          530 t
ORIGIN      
        1 acggcctatt tgctgaatga gtctcccgat ggccgggtcg agctccaaaa ggacgggcaa
       61 ttcgctttta cgccgaaaga cgggtttacg ggtaattcca cacaattcag ctataaagta
      121 tgtgataacg ggtcgcccgc actgtgttcc atcacgacga cggccgtgat ccggttcccc
      181 agcaagagca cgcttgtcag cttcgatgcc ctgtacagca ggaaagaagt ggcgatcaac
      241 tggaatatcg ccaccgacaa ccatagcaag acgttcgagg tggagcggtc gctggacggc
      301 agcttctata agaaagtggg tgaagtgaaa gcggaagagg cgatcatgga aggaaaatat
      361 acgttcaatg ataagatgca tgaggccgag cggaggaacg atctttatta ccggcttcgc
      421 cagatagacg gtgcgggtcg cgttacctat tcgaaggtat tgatcatgcg gtcgtatggt
      481 agcaagagtg tggaagcggt gagcgttacg ccggatccgg gtgtcaacga catccaggtg
      541 aacgtgcagc tgaaagaaaa atcgtttgta gtaatgcggg taaccgacaa caatggcagc
      601 gagctgatca agcagactgc gatgggtgat ctcggtacca atcaatataa tatcgaaggg
      661 acttcgcagc tacagccggg gttgtaccag ctggaggtga tcatcaacag taatgagcgg
      721 atgacgatga agctggcgaa aagttaatgg gttttagggg tataatgggg ccgcccttcg
      781 ggcggcctta tttttttatg ttcaggtagg tctgcagatc ttttacatat tcttcgaagg
      841 cccggatgcc ttccggcgta atccggcaaa tggtctgggg atagttgtcc ttgaaggatt
      901 tggtgacgtc gatatagccg gcctctttga gcttgttgat ctgcacgctg aggttgccgg
      961 ccgtggcatc ggtcttttcg cggagatagg tgaactccgc ctccttcacc gagatgagga
     1021 tggaaaccac cgccagacgc agctgtgaat gtaatatagg gttgagatct ttaaacatgt
     1081 tgttttttgg ctttcagata gcatttccga aggatgagac cgggtatgaa ccaggcgagc
     1141 tgggcggcgg cggtcatgca caagatcttg tagggtaaag gcgtgtaatg gcaaaggatg
     1201 gccagcagcc agcaggccag gccgccgatg atcattggtt taaaccggcg ggcgtagccc
     1261 gtagcgaagg taggtacgcc gtagagggtc aggaacaggg gcccctcttc gttgacctgg
     1321 taggcattgg tgatatacga cagaaggaat atggcgatgc cgaagctgat ccagatgccc
     1381 cccatgatat cctcgtcata ggcccggtgt ttccttttct tcgcttcccg gatggcgata
     1441 atgatctgtg ggacgacggc cgctatggtc aggaaccaga cgtaatcgaa taccggttgt
     1501 ttgatccagt agttggcaaa ggtgacgaga gagcagaagg tgatgacgga tccccagagt
     1561 aaggcgctga gtcccgaatc gagatagtct cttcgggcct tgttgatcat tttcgtgata
     1621 agtgccaggc tttcctggcc ggtgagttct ttttcttcca taaattaaat ttaccaatgt
     1681 ggggccggct tcgccggccc cacttaaaat ttattgacac tgcgcgagcc cttgctccgc
     1741 ctgcggcaga ccccaggtgg gatacaacgg cttgggttgc gtggacttga acagggcgat
     1801 cgatttctcg aagaccggtt tggccttgtc cttgccgccg ccgaattgag gaggggttcc
     1861 aaacaggctc atggcctgaa gatagtataa ccgcgggtta ttcggatcga tggacatacc
     1921 tttctgaagt gcatccgaag cgtcctttcc gttggtggcc caacgtgtct gcgggtccat
     1981 cagcatttgc tgtgtggaga gcatattttc gacggcataa gtttcggcgt tcttctcgat
     2041 ggcttcggcc ttggaaagat aggatttgat cttggcggca ttgccgtcct tgtccttggt
     2101 ttcggggttc caacctaccc aggtttgcgc cagggcagca tagtaatagg gcagccattg
     2161 ggtcttttcc gcgtcgccga tgcgatcg
//
LOCUS       DASYSV010000229         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2346909, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000229 DASYSV010000000
VERSION     DASYSV010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2346909"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VGQ51_07110"
     CDS             complement(<1..1167)
                     /locus_tag="VGQ51_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036368.1"
                     /translation="MLIHYLRSAWRTIRRNPFYSLINIGCLAIGIAVCMTVLLYILHE
                     HSFDRWQKNARRIYGVTVNIHFGDGSFNTPGISYATGPLVRQADGNVEAWLRMMPVTE
                     PVAVKDAVAPEKDFGQERKLVYADSNFFDFFSFHLLRGNPASVLRRPFTVVLTETAAK
                     RYFGDKDPIGRTLRIYDRYNFEVTGIAADLPSNTDIDFDAVVSMASMRSMDNYRNWMS
                     SQHQRVQGGAFHTWLLLRGQDVAGQVEATMDRLAIVPGEPSNTRDAYTLTSLADYHLH
                     SKVVDTSNLRYLTIFQLAAGLILLLALVNYMSLATARATARAKEVGVRKVMGAGRGRI
                     AGQFYTESAVFAVSAFLAGTLIFLSIRPVFFGWLQLPIDTALLWSPGMLAFSGAL"
     gene            1206..2292
                     /gene="prfB"
                     /locus_tag="VGQ51_07115"
     CDS             join(1206..1265,1267..2292)
                     /gene="prfB"
                     /locus_tag="VGQ51_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_124635252.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV9036369.1"
                     /translation="MTQEQIKSMRDRVVVLRRFLDVDNRQYKINEEKQLSVSPGFWDD
                     NRRATEILKNIKLNEYWVKLYESVDSSVEDFVVLFEFWKAGEASEEETREAYDKAMSR
                     IEEVEFKSTLNQPEDELPAVMQINSGAGGTESQDWAEMLLRMYRMYGEKQGWSVTELD
                     LQDGEGAGIKSATLQFDGPFAYGFLKAESGVHRLVRISPFDSNARRHTSFASVFVYPL
                     VDDSIEIEVSPADLEWEFYRSGGKGGQNVNKVETAVRLKHIPSGIIVECQQARTQGEN
                     REKALKMLKSRLYEEELRKREELKNATNSTKKKIEWGSQIRSYVFHPYKMIKDHRTDY
                     EVGNVQPVMDGELDGFIKAYLMNEKEN"
     gene            2310..>2721
                     /locus_tag="VGQ51_07120"
     CDS             2310..>2721
                     /locus_tag="VGQ51_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV9036370.1"
                     /translation="MSLGTFNYPAPHNEPVLSYAPGTSERETLKKALQQLKSTEHDIP
                     MYIGSQEVRTGKKAALRPPHETAHVLGYFHQGDETHAQQAIDAALQAKAGWASLPWED
                     RAAIFLKAADLISTKYRAYMNGTTMLGQSKNAFQA"
BASE COUNT          705 a          801 c          725 g          490 t
ORIGIN      
        1 cagcgcgccc gagaaggcca gcatccctgg tgaccacagc aatgcggtat cgatcggcag
       61 ctgcaaccac ccgaagaaca cgggccgtat cgataaaaag atcagcgtcc cggccaggaa
      121 cgcggacacc gcaaagacgg ccgattcggt atagaactgt ccggcgatcc tgccccgtcc
      181 cgcgcccatg accttccgga ccccaacctc tttggcgcgc gctgtcgcgc gcgccgtagc
      241 caggctcata taattgacca gcgccaagag caggatcaaa cccgccgcca gctggaagat
      301 cgtcagatac cggagattgg aggtatctac caccttactg tgcaaatgat aatcggcgag
      361 ggacgtcagc gtatacgcat cccgggtatt cgacggctcg ccgggcacta tcgccaaccg
      421 gtccatggtc gcttccacct gaccagccac atcctgaccc ctcagcaaca gccaggtatg
      481 gaaggcgccg ccctggacac gctgatgctg cgagctcatc caattcctgt aattgtccat
      541 cgaccgcatg ctggccatcg atacgacagc atcgaagtcg atatcggtgt tggaaggaag
      601 atcggcagcg atcccggtca cctcgaaatt gtatcggtcg tagatccgga gcgtgcgccc
      661 gatggggtcc ttgtccccga aatatctttt tgccgccgtc tcggtcaata cgacggtaaa
      721 aggccgccgc aacacactcg cgggattgcc cctcaaaaga tggaaactaa aaaaatcgaa
      781 aaaattgcta tccgcataga ccagcttcct ttcctggcca aaatcctttt ccggcgccac
      841 cgcgtccttt acggccaccg gctcggtgac cggcatcata cgcagccagg cctccacatt
      901 accgtcggcc tgccgaacaa gcggccccgt agcatagctg atccctgggg tattgaagct
      961 cccatccccg aagtgaatgt ttaccgtcac cccgtatatc cgccgcgcat tcttctgcca
     1021 gcggtcaaaa ctgtgttcat gcaggatata cagcaatacg gtcatacaaa cggcgatgcc
     1081 gatggccaaa cagccgatat tgatcagact gtagaaagga ttgcgacgga tggtccgcca
     1141 cgcgcttcgc aggtaatgga taagcatacc tgaaaataac caatttttct tacctttgcc
     1201 agcttatgac acaagaacag atcaaatcta tgagggaccg cgtggtcgtc ctgaggaggt
     1261 ttctttgacg tcgacaaccg tcaatataag atcaacgaag aaaagcagct ttcggtaagc
     1321 ccgggtttct gggacgacaa ccggcgggcg acagaaattc tgaagaacat caagctgaac
     1381 gaatactggg tgaagctgta tgaatccgtg gactcctccg tggaggattt cgtcgtgctt
     1441 ttcgaattct ggaaggcggg cgaagcctcc gaggaagaga cgcgcgaagc ctatgacaag
     1501 gccatgagcc gcatcgagga agtggaattc aaaagcacgc tcaatcagcc cgaagacgaa
     1561 ttgccggccg tcatgcagat caattccggc gccggcggca ccgaaagcca ggactgggcc
     1621 gaaatgctcc tgcgcatgta ccgtatgtac ggcgaaaaac agggctggtc cgtcaccgag
     1681 ctcgatctcc aggacggaga aggcgccggt atcaaaagcg ccacgctcca gttcgacggt
     1741 cccttcgcct atggctttct gaaggccgaa agcggcgtcc accggctggt acgcatctcc
     1801 cccttcgata gcaacgcgcg ccgccatact tccttcgcat ccgtcttcgt ctatccattg
     1861 gtggacgaca gcatcgagat cgaggtcagc cccgccgatc tggaatggga gttctaccgc
     1921 agcggtggta aaggcggaca gaacgtcaat aaggttgaga ccgcagtccg gctgaaacat
     1981 atccccagcg ggatcatcgt ggaatgccag caggcaagga cacaggggga gaacagggag
     2041 aaggccctga aaatgctcaa aagccggctc tacgaggaag agctccgcaa acgcgaggag
     2101 ctgaagaatg cgaccaattc caccaagaag aagatcgaat ggggctcgca gatccgcagc
     2161 tatgtattcc atccttataa aatgatcaag gaccaccgga ccgattatga ggtcggcaac
     2221 gtgcagccgg tgatggacgg agaactcgac ggattcatca aggcttacct catgaacgaa
     2281 aaagaaaatt aattaaacaa ttcaatagca tgagcctcgg aactttcaat taccccgcac
     2341 cgcacaacga accggtcctg agctatgccc cgggcaccag cgaaagggag accctgaaaa
     2401 aagcgctcca gcaactcaag agcaccgaac acgacatccc gatgtacatc ggctcacagg
     2461 aagtgcgcac aggaaaaaaa gcagccctcc ggccgcctca tgaaacggcg cacgtcctcg
     2521 gttattttca ccagggcgac gaaacgcatg ctcaacaggc catcgatgcc gcgctgcagg
     2581 caaaggcagg atgggcctcg cttccctggg aagaccgggc tgcgatcttt ctcaaggcgg
     2641 ctgatctcat ctcgacgaaa taccgtgcct atatgaacgg cactaccatg ctcggacaaa
     2701 gcaagaatgc attccaggcc g
//
LOCUS       DASYSV010000230         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_297298, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000230 DASYSV010000000
VERSION     DASYSV010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297298"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VGQ51_07125"
     CDS             <1..790
                     /locus_tag="VGQ51_07125"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV9036371.1"
                     /translation="VNVQEYIESGIIESYVMGLASESERAEFERLCSQYPELVVARKK
                     FEESLEGYASENATPPPPEVKAKVLEAVKMLPTRRGPGTTAGRTQIQILLRLIIAAFF
                     VLLVGMTFLYYQTRQENQDLTNINQRLKETLDTTSTILKKIVDEQKVVSSPDVTVVNM
                     VGTQAAPKSSANVYWDSTRSDVYLVVKNMPGLPSDQQYQLWALIDNKPKDLGVFDATD
                     DKVILKMKNTQKAQAFAITIEKKGGSNSPTLQKMQSMGKTMQTQ"
     gene            906..1319
                     /locus_tag="VGQ51_07130"
     CDS             906..1319
                     /locus_tag="VGQ51_07130"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV9036372.1"
                     /translation="MVIFLRKYFAALYVPITWTIIIGILMTLPGSALPNEQAFRIPQF
                     DKIVHMALFGGFVFLWDLRLSKRSQDMRRLFRWFFAFYVLANVYGIGMEYVQKYWIPG
                     RDFDLADIIADMIGAGLGYGFSHLLLLPTEKNKNG"
     gene            complement(1725..2789)
                     /gene="prfA"
                     /locus_tag="VGQ51_07135"
     CDS             complement(1725..2789)
                     /gene="prfA"
                     /locus_tag="VGQ51_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV9036373.1"
                     /translation="MLDKLAAIKARFEDLGVALSNPEIVSDNRKFSAMSKEYRSIEKI
                     VLAYQEYKDLLDTMDFNKEALSGDDPELRELAKAEAPTLEERKLHLEAHIRQLLIPKD
                     PQDEKNAILEIRAGTGGDEASLFAGDLLRMYIKYCEKRGWKTAILSESEGTVGGYKEV
                     QMEVVGDDVYGTLKFESGVHRVQRVPDTETSGRVHTSAATVAVMPEAEEVDFELKESD
                     VKMETARSGGAGGQNVNKVETKVFLTHIPTGVTVVCQTERSQLGNREKAMQMLRTKLY
                     EDQVRKQEEEIARHRKSLVSTGDRSAKIRTYNFPQGRVTDHRIGLTVYNLDDVLNGNV
                     QEFIDALQFAENAEKMARQN"
     gene            2869..4032
                     /locus_tag="VGQ51_07140"
     CDS             2869..4032
                     /locus_tag="VGQ51_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9036374.1"
                     /translation="MTYRKFKADYLFTGEGLAAPESVLITTTDGAVQGVVPFSDAGDG
                     IECYTGLISPGFVNCHCHLELSHLRGVIPEGTGLVDFLTGVIHTRNQPGDKDAVQRAI
                     AEGEREMLDGGIVAVGDICNTTDTLEQKAKAGLYYHNFIETIGFNETSAQERWAHSLA
                     VYDRFGAVGSIVAHAPYTVSPALFRLIAALPGNRLQTIHNQESEEENLFMTYASGDLL
                     RLYRQLGIDISFFEGFGKRSLAVWPSYFHRHQTVIAVHNVATSKEDLEGLPKDGPTLH
                     FCLCPRANLYISGRLPDIDVLLRQDCRIVIGTDSLASNKGLSVLEEIKTLQRSNPRLD
                     TPLLLKWATFNGARALGIEDIFGSLKPGRRPGVLLLSYAEDGSLKEATVRRLL"
     gene            complement(4029..>4505)
                     /locus_tag="VGQ51_07145"
     CDS             complement(4029..>4505)
                     /locus_tag="VGQ51_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HEV9036375.1"
                     /translation="FDVFKNAVASFMVELLQKCLKQPEPNPDLFYFAEDAFVHLDDSD
                     GRVMANLALFFALHLAGFFGFQIEDIYSEHVSFFDLREGRFVDEHPQHPYMLEGNLAY
                     ATAQLLRVRQPAELREIALNQEMRRALLQAYRTFYALHVADFGEMRTLAVLQTVLS"
BASE COUNT         1076 a         1157 c         1179 g         1093 t
ORIGIN      
        1 agtgaacgta caggaatata tcgaaagcgg catcatagaa agctatgtga tgggactggc
       61 cagcgagtcg gagagagccg aattcgaacg attgtgtagt caatatcccg agttggtggt
      121 tgcacgaaaa aaattcgagg agagcctcga aggctatgcc tcggaaaatg cgactccccc
      181 tcccccggag gtaaaggcaa aagtcctcga agcggtaaaa atgctcccga cgcggcgggg
      241 acccgggacc acggcgggcc ggacccagat acagatactg ctacggctca tcatcgccgc
      301 ttttttcgtg ctgcttgtcg ggatgacctt tctgtactat caaacgagac aggaaaacca
      361 ggacctcacc aatatcaatc aacggttgaa agaaacgctt gacacgacga gtactatcct
      421 caaaaagatc gtggatgaac agaaagtggt cagcagcccc gatgtaacgg tggtcaatat
      481 ggttggaacg caggctgcgc cgaaatcgtc tgccaatgtc tactgggatt ccaccagatc
      541 cgatgtctac ctcgttgtga agaatatgcc cggactgcct tcggaccaac aataccagtt
      601 gtgggccctc atcgacaaca agcccaaaga tctcggggta ttcgacgcta cggatgacaa
      661 ggtcattctg aaaatgaaga atacacaaaa ggcgcaagcc ttcgccatta ccatcgaaaa
      721 aaagggcggg agcaattctc cgaccttaca gaagatgcag tcgatgggaa agaccatgca
      781 aacacaatga cgacctgccc gagagcgcat taccgatgtt atgtggcgtc gacaatagtg
      841 gaaaataatc tacattccag ctaaaaaagt ccccaaaatc actgcatccc ggcctatatt
      901 cgtgtatggt catctttctc aggaaatact tcgccgcatt gtatgtaccc ataacctgga
      961 cgattatcat cggaattttg atgaccttgc ccggttcggc cctgcccaac gaacaggcgt
     1021 tcaggatccc ccaattcgac aagatcgtgc acatggctct ctttggaggc ttcgtattcc
     1081 tctgggacct gcgtctgagt aaacgatccc aggatatgag gcgtctgttt cgttggtttt
     1141 ttgccttcta tgtcctcgcc aatgtctatg gtatcggtat ggaatacgtc cagaaatatt
     1201 ggatcccggg acgtgatttc gatcttgccg atattatagc ggatatgatc ggggccgggc
     1261 tcggatatgg gttcagtcac ctgttgttgc tgccgacgga gaaaaacaaa aacggttagc
     1321 cggcaacgtt acatcgcctt cctccaaaaa ataaacccct gtagaaacag gggtcgtaac
     1381 caaaactaac tgcttatgag aaaattgact gctttatgtg agtcaaatag aaaagactgt
     1441 ttgatgtgta taacgcaaaa caggtgccaa tattgtatgt ctcaacctcc gttccccgtc
     1501 attcactgcc caaaattacg tcggaattcg acttcgggtt tccccttgcg ggaggaataa
     1561 catttattta gcattgctct cttaagttaa gcaaattcct gatctatagc aagttttagc
     1621 gctttttttt aaagaacttc taaaagaacc ttcatgataa tagacaagaa aaggacccga
     1681 aagtttaggc tccgacccgg ccggtcggcg tttttaatgc ggctttaatt ctggcgggcc
     1741 attttttccg cattctcggc aaattggagg gcgtcgatga attcctggac attgccattg
     1801 aggacatcgt cgaggttata gaccgttagg ccgatacgat gatcggttac ccgtccctgg
     1861 gggaaattat aggtcctgat cttcgcgctc cggtcgcccg tgctgacgag gcttttccgg
     1921 tgcctggcga tctcttcctc ctgtttgcgg acctggtctt cataaagctt ggtgcgcagc
     1981 atctgcatgg ctttttcgcg gttgcccaac tggctgcgtt cggtctggca gacaaccgtt
     2041 actcctgtag ggatgtgggt tagaaagacc ttggtttcga ccttattgac attctggccc
     2101 cctgcccctc cgctgcgtgc ggtttccatt tttacatcac tttctttcaa ctcgaaatcc
     2161 acctcctcgg cttcgggcat gaccgcgacc gtcgccgcgc tggtatggac ccgaccgctg
     2221 gtctccgtat cgggtacccg ctgaacgcga tgtacgccac tttcgaattt cagggtgccg
     2281 taaacgtcat cgccgaccac ttccatctgc acttccttat acccgccgac agtaccctcg
     2341 ctttcgctca aaatggctgt tttccagcct cttttttcac agtatttgat atacattcgc
     2401 agcaggtctc cggcgaagag actggcttcg tcgccgccgg taccagccct tatctccaaa
     2461 atggcatttt tctcgtcctg cgggtctttg ggtatcagga gctggcggat atgggcttcc
     2521 aggtgcagtt tgcgctcttc cagggtcggg gcctcggctt tggccagctc ccgaagctcc
     2581 ggatcgtctc cgctcagggc ttctttgttg aagtccatgg tatcgagaag gtccttatat
     2641 tcctgatagg cgagcacgat cttttcgata ctgcggtatt ccttgctcat ggcgctgaat
     2701 ttccggttat cgctgacgat ctccggatta ctgagcgcga ctcccaggtc ttcgaaccgg
     2761 gcttttatgg ctgcgagctt atctaacata tccatcggtg cttttagccg gggccatgag
     2821 gaaaagtgtg cagatggccg gcggaccgca aagataaaat gtttgggaat gacttatcgc
     2881 aaattcaaag ccgattattt atttaccggg gagggcctgg ccgcgccgga aagcgtactg
     2941 atcacaacca ccgatggcgc ggttcagggt gtagttccat ttagcgatgc aggcgacggg
     3001 atcgagtgtt ataccggcct tatcagtccc ggtttcgtca attgccattg tcatttggag
     3061 ctgtctcatc tcaggggcgt aatacctgaa ggcaccggcc tcgttgattt tctgacgggt
     3121 gtgatccaca ccaggaatca gccgggcgac aaggatgctg tgcaacgtgc cattgccgaa
     3181 ggcgaacggg aaatgctgga cggcggcatc gtggctgtag gggatatctg caataccacc
     3241 gatactctgg aacaaaaggc gaaggcaggt ctttattacc acaatttcat cgagaccatc
     3301 ggcttcaatg agacgtcggc gcaggaaagg tgggcgcata gcctcgccgt ttatgaccgg
     3361 ttcggcgcgg tgggttcgat cgttgcgcat gcaccctata cggtctcacc cgcactgttc
     3421 cggctaatag cggcattgcc aggcaacagg ctccagacga tacacaacca ggaaagcgaa
     3481 gaagaaaacc tgttcatgac atacgcatct ggagacctcc tgcgtctata ccggcaattg
     3541 ggtatcgaca tatccttttt cgagggcttc gggaaaagga gcctggcggt ctggccttct
     3601 tattttcacc gtcaccaaac ggtgattgcc gtacacaatg tggcaaccag taaggaagat
     3661 ctggaagggt tgccgaagga tgggccgaca ctgcatttct gtctttgtcc ccgggcgaat
     3721 ctttatatca gcggcaggct tcccgacatc gacgtcctcc ttcgacagga ttgccggatc
     3781 gtcataggta ccgacagcct ggcgtcgaat aaagggctta gcgttctcga agagataaaa
     3841 acgctgcagc gatcgaatcc ccgattggat actcctctcc tgttaaaatg ggcaaccttc
     3901 aatggagcaa gggcgctggg tatagaggat atcttcggga gcctcaaacc gggaagacgg
     3961 ccaggggttc tgctgctgtc ctatgcggaa gacggcagcc tgaaggaggc cactgtccgc
     4021 cgcctgcttt aggacaagac ggtctggagt acggcaagcg tacgcatctc tccgaaatcg
     4081 gctacatgaa gtgcatagaa ggtccgatag gcttgaagga gagcgcgacg catttcctga
     4141 ttgagcgcga tctcgcggag ctcggccggt tgccgtacac gcagcagttg cgccgttgca
     4201 taggcgagat tgccttccag catataggga tgctgaggat gttcgtcgac gaagcggccc
     4261 tctcttaaat cgaaaaagga cacatgttcg ctgtagatat cctcgatctg gaaaccaaaa
     4321 aagccggcca ggtgcagtgc gaaaaaaagt gctaagttcg ccatcactcg tccatcgctg
     4381 tcgtccaggt gtacgaacgc gtcctctgca aaataaaaaa gatccggatt gggctcgggt
     4441 tgtttgagac atttttgaag caattccacc atgaaagaag caacggcatt cttgaatacg
     4501 tcgaa
//
LOCUS       DASYSV010000231         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2088559, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000231 DASYSV010000000
VERSION     DASYSV010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088559"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VGQ51_07150"
     CDS             complement(<1..920)
                     /locus_tag="VGQ51_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036376.1"
                     /translation="MIKNYILLAWRNLLKHRSSSMINIGGLALGLTTSILVVLFIVNE
                     FGYDSFHRNIDYLYILMKNQKVADGISTGGASAGPMAAALRTELPEVQNVAREVGAGT
                     TARVGDKQVPLEGIYTDTGFFSMMSFPAIEGDPGSALRDPHAVILTESAAKKLFGNSP
                     ALGKTFSDDTVPVRVGAVVKDVPSNSSIRFEYVRPIAVFESNNPWLKKWDDNRINTWL
                     QLKPGADIAAVDRKATAILQKRSDDTSVTTFVWPMARLRLHSHFENGKPAGGKIYMVL
                     LIALLGFFVLIIACINFMNIATARSEIRAR"
     gene            1172..2248
                     /locus_tag="VGQ51_07155"
     CDS             1172..2248
                     /locus_tag="VGQ51_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV9036377.1"
                     /translation="MEADELLKKKPVPAIRLVVQMALQLLMLALLLGWSYHILFPFVT
                     PIVWGAVLAVALYPIHQRLKVALKGKGGFAAVIICICMLALVILPAVILMLNTADEAR
                     EAMTAFQEGKLKIPPPGDNVKNWPLVGRKADAVWRQASDNFSLLVEKNPEKVKSVARK
                     VVGLVGSTATGIGLFAVAVIIMAVFLSYSTKASNFARSLFSRLMNSSTFDMPAIAATT
                     IRNVVKGILGVAFIQTLLFAAGLWLADIPYAGIWTLCCLVLAIVQLGTLPIAVGVIVY
                     IWGQDNTLAAILLTIWVVIVSLLDNVLKPILMGKGAPVPMLVVFLGALGGFMYMGFIG
                     LFIGAVILSLGYKLFEVWLAGTEI"
     gene            2308..2505
                     /locus_tag="VGQ51_07160"
     CDS             2308..2505
                     /locus_tag="VGQ51_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036378.1"
                     /translation="MPDREKMTSGRDADHLVPGANRLLSIAAKTAWRATKLIAGIVIR
                     IPGWFVHANKRHISGRNGGSE"
BASE COUNT          552 a          683 c          681 g          619 t
ORIGIN      
        1 tctctcgccc ggatctccga tctcgccgtg gcgatgttca taaaattgat acaggcgatg
       61 atcagcacga agaaacccaa caacgcgatc aatagcacca tgtatatctt cccgccggcg
      121 ggcttcccat tttcgaaatg agagtgaagc ctcaaccggg ccatgggcca gacaaaggtc
      181 gtcacgctgg tatcgtcgct acgcttttga agaatggctg tcgccttccg gtcaacggcg
      241 gcgatatcgg ctcccggttt caattgcagc caggtgttaa tccggttgtc gtcccatttt
      301 ttcaaccagg ggttattgct ttcgaatacc gcaataggcc gtacatactc gaatcgaatg
      361 ctgctgttgg aagggacgtc cttcacgacc gcaccgacac gcaccggtac tgtgtcgtcg
      421 ctgaatgttt ttccgagtgc ggggctattc ccaaacagct ttttcgccgc actctcggtc
      481 agtatcacgg catgcgggtc gcgaagcgcc gatccgggat ctccttcaat tgccggaaag
      541 ctcatcatac tgaaaaaacc ggtatccgta tagatacctt cgaggggcac ctgcttatcc
      601 cccacgcgtg ccgtagtccc cgcacccacc tcgcgcgcaa cattctgcac ctcgggcagt
      661 tccgtcctca aagcggccgc catcggtccg gcgctggctc cgcccgtcga tatcccgtcg
      721 gccacctttt gattcttcat caggatatac agatagtcga tattccgatg aaaggaatcg
      781 tagccgaatt cattcactat gaaaaggacc accaggatac tcgtcgtcag cccgagggca
      841 agtccgccga tattgatcat ggaggagctg cggtgtttga gcagattgcg ccaggcaagc
      901 aggatatagt ttttgatcat gaaaaagaca gttggaactg atccgggtgc caaacacatg
      961 ccaatcaaac aaatcattga tagtcagcga atggaggaga tcgaggtcgt tcagactgtg
     1021 cgaaatcgat acgccggctg tccgccgtca gggaagcccc gcccctatct ctatttaacg
     1081 gtaaagacat tatcggatct gaaaatgctt gtatttttag ctttgccttt tccatgatca
     1141 gaagcccctg cccatatcta aaggattctt tatggaagca gacgaacttt taaagaagaa
     1201 accggtcccg gctatacgcc tggtggtaca gatggcactt cagcttctta tgctggcctt
     1261 gttgcttggc tggtcctatc acattctttt tccgtttgtc acccccattg tctggggtgc
     1321 ggtgctggcc gtcgcccttt atcccattca tcagcggctg aaggtcgcgc tgaaaggaaa
     1381 gggtgggttt gccgccgtga tcatctgcat ctgcatgctt gctttggtca tcctccccgc
     1441 cgtcatcctg atgctgaata cggcggatga agcgagagag gcgatgacgg ctttccagga
     1501 aggcaaacta aagatccctc cgccgggcga caatgtcaaa aactggccgt tggtgggaag
     1561 gaaggcggat gcggtgtgga gacaggcctc ggataacttc tcgctgctgg ttgaaaagaa
     1621 tccggaaaag gtgaagtcgg tggccaggaa agtcgtggga ctcgtcggaa gtacagccac
     1681 cgggatcggc ctttttgcgg tggccgttat catcatggct gtttttctgt cctattccac
     1741 aaaggcatcc aattttgcac gatcgctttt cagccggctg atgaacagca gcacattcga
     1801 tatgccggcc atcgcagcca ccaccatccg aaatgtcgtg aagggtatcc tgggcgtcgc
     1861 attcatccaa accttgcttt tcgccgccgg gctttggctc gccgatatcc cttatgcggg
     1921 gatctggact ctatgctgcc tggtcctggc gatcgtacaa ttgggtacgc ttcccatagc
     1981 cgttggcgtt atcgtctata tatggggaca ggataatacg cttgcagcca tcctgctgac
     2041 gatctgggtg gtcatcgtca gcctgctgga caatgttctc aaacccatcc tgatgggcaa
     2101 aggcgcgccg gttccgatgc tggtcgtttt tttaggcgca ttgggtgggt tcatgtatat
     2161 ggggttcatc gggctgttca tcggcgcggt gatcctttcg ttaggttata agctgttcga
     2221 ggtctggctc gcgggcacgg aaatctgaaa aatgaatccc gggctccgca tggcataggt
     2281 tttgaaatgt tttttttaaa atgggccatg cctgatcgcg aaaaaatgac ctcgggacgc
     2341 gacgccgacc acctggtccc cggcgccaac cggctcctgt ccatcgcagc aaaaactgcc
     2401 tggagggcga caaaactcat cgcaggaatt gttattcgga tccccggctg gttcgtacat
     2461 gcgaacaaac gacatatttc cgggcgaaac gggggttccg agtgaaccta gtgtactccg
     2521 tcaaaggccg tgcgg
//
LOCUS       DASYSV010000232         4326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1449479, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000232 DASYSV010000000
VERSION     DASYSV010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449479"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VGQ51_07165"
     CDS             complement(<1..473)
                     /locus_tag="VGQ51_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV9036379.1"
                     /translation="MNRNNLLLLVMFFAGCGDRQEAPKTPENGRLRIVSVSKQLTEII
                     FAVGGDTALVGRDISSTWPPAATKITAVGYHRALGSEGIISLHPTVLWNDGNWGPQST
                     IDQLKKVGIPLRQFDGGNTIDSTERLIRQIAAEFHNPRAGDSVCQHLDADMKRPDS"
     gene            complement(489..2633)
                     /locus_tag="VGQ51_07170"
     CDS             complement(489..2633)
                     /locus_tag="VGQ51_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9036380.1"
                     /translation="MLERTGVFLLFIFIALSTAAQRAPKTANDTLKPQTLNTVQVTGW
                     STGKKDNQLDVAHSVGILTAEDFHRNTGFSLENSLNLMPGVSMGSRTSFGGQRIIIRG
                     YGNNTNFNGQGVQVLLNNIPVTDATGTTILDDIDFSTLGKVEVIKGPASSLYGSGVAG
                     VVNLFTLKPQPGQARIVEENTGGSYGLWRNNTRIESANEHSSLLFNYGHQEEEGWRTN
                     SASRKDYAQFNGDFFVSDKNTISAFFAYARSLEQLAGEMTDSIIYHRIAWSDPAYLVN
                     GSKTVIESVRAGMTDNYKIDDHFSNQTTLFTNAYTLNQPFAHGLTDNQAFTFGGRTGF
                     VYTSDTGSVTVHGILGAQFQKTTVFNKSYNLTIGALGGIRGDLQNASQGYSVFTEWKF
                     SLPSQVVLTAGGSLNFTEYGITDMLANSANPTHANQGGYKSFSPVFTPRISVLKMLGD
                     HVSIYADVSAGYTPPPTSQVINAVVGTTNKDLKPESGIQYEIGTKGDLFASKLSYQLA
                     LFDLDVHNKIVAEQVPASGSTPTYSTYVNAGRQQNLGAELAVSYSLVNDPRRVISLLR
                     PFMTYTYSDFRYKSFYSDNNHNAATLDFSNNQVAGVAPNVVNIGIDAQTRAGFYLYLT
                     YRHVDAVPYTFDNRNYARAFNLLNGKLGYRSQLGRHFSVDAYAGVDNLTNCTYYSFLF
                     FSGTLAGTTDPHFLPMPYNAAFYGGAKLAYIF"
     gene            complement(2666..3286)
                     /locus_tag="VGQ51_07175"
     CDS             complement(2666..3286)
                     /locus_tag="VGQ51_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036381.1"
                     /translation="MKRSFKVFRVSNEADFRPKSWIFATAKTSCLKRSIAILLLLPLC
                     YTQFGFYGQTIYLQWRMKEAANEARIAALPDAVMLRVSVAELNASGRWEDEGRECWYK
                     GHLYDVIRQRTVSGTTWLYCLDDEGEEQLIHGAVDVTKANQDQPGRQTRISFSICDLL
                     CQTPQWTIAPLQPIDRQYPTYGVHRLSSRYARIVIPPPKASAWLFG"
     gene            complement(3201..>4326)
                     /locus_tag="VGQ51_07180"
     CDS             complement(3201..>4326)
                     /locus_tag="VGQ51_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036382.1"
                     /translation="NALYFSPDDGASWVHLRNNLPPAPIYGLTIQRNFKDLVIGTYGR
                     GFYILDDITPIRDFSDSVRQSEAWLFPMRKAWRFRPKPAIHGERSASFGVNPPYGADI
                     NYYLRDTVRDTVKVLILDSAGRTVQQIAGSNRKGVNRVWWDLGLQPYVLPPLRTRPED
                     ADWVKLDSTGQRAMVIYDLDIGPGLPATKVLPGTYTVVLKIGRKEYRQPVQVFRDPHT
                     GGSDEDIHEQYAFGVQIYGSIQSILHMIDTLEIVRNRADSIGRLYTDRRQKAKLQAWK
                     DQLYEAEALLHDVHQTGAREDIFRNPAQLLERFLTISKESISGGADFRPTNQHKEVYA
                     LLSGRLDAARKKYEAVLQSFPRLKRGGLPPEKLDIRNSKN"
BASE COUNT          957 a         1190 c         1166 g         1013 t
ORIGIN      
        1 gagtcgggcc gcttcatatc ggcatccaga tgctggcaga cactgtcgcc tgcacgcggg
       61 ttgtgaaatt cagcggcgat ttggcggatc agccgctcgg tcgagtcgat cgtgttcccc
      121 ccatcgaact gtcgcagggg aattcccact tttttcaatt gatcgatggt cgactgcgga
      181 ccccagttgc cgtcgttcca cagaacagta ggatgcagag aaatgatccc ctcggaaccc
      241 agtgcgcgat gatatcccac cgccgttatt ttggtggccg ctggaggcca ggtgctcgag
      301 atatcccttc ctaccagggc agtatcaccg ccgacggcaa aaatgatctc ggtcagttgc
      361 ttgctgacgc ttacgatccg cagccgccca ttctcggggg tcttcggggc ctcctggcgg
      421 tcgccgcaac ccgcaaaaaa cattaccagc aacaaaagat tatttcggtt catacggttg
      481 tttttggatc aaaagatata agccagcttt gcccccccat aaaaggcggc attgtagggc
      541 atcggcagaa aatgcggatc ggtcgtaccc gccagtgttc ccgaaaagaa cagaaagctg
      601 taatacgtgc aattcgtaag gttatcgact cccgcatagg catccaccga aaaatgccgg
      661 cccagctggc tccggtatcc cagtttcccg ttcaaaaggt tgaatgcccg cgcataattc
      721 ctgttgtcga acgtatacgg aacggcatcg acatgccggt aggtcagata aagatagaat
      781 cccgcccgcg tctgcgcatc gatgccgatg ttgaccacgt tcggcgccac gcctgcgacc
      841 tggttattcg aaaagtcgag tgttgcggcg ttgtgattgt tatcgctgta gaagctctta
      901 taccggaaat ccgaatacgt ataagtcata aatggacgca aaagcgagat cacccggcgc
      961 ggatcattca ctaaggaata gctcaccgcc agttcggcac caaggttctg ctgtcgcccc
     1021 gcattgacat atgtgctata ggtgggagtg ctgccggaag caggcacctg ttcggccacg
     1081 atcttgttgt gaacgtcaag atcgaacaaa gccagctggt aggataattt cgacgcgaac
     1141 agatcccctt tcgtaccgat ctcgtattgt attcccgatt caggcttcag atctttgttg
     1201 gtcgttccca cgaccgcatt gatcacctgc gaagtaggcg gcggggtata cccggccgag
     1261 acatcggcat agatggaaac atggtccccg agcatcttca gcaccgatat cctgggggta
     1321 aacacagggg agaaggattt atatccgccc tgattggcat gcgttggatt ggcggaattc
     1381 gcaagcatat ccgtgatccc atattcggta aagttgagac tgcctccggc ggtcaggacc
     1441 acctgcgagg gcagcgagaa tttccattcc gtaaagacgg aatatccctg cgaggcgttt
     1501 tgcagatcgc ccctgatacc gcccaacgca ccaatagtca ggttatagga tttgttgaag
     1561 accgtggtct tttggaattg cgcgccgagg atgccatgaa cggtcacgct gccggtatcg
     1621 gaggtataga cgaaccccgt cctgcccccg aacgtaaagg cctggttgtc cgtcaaccca
     1681 tgcgcaaacg gctggttcag cgtatatgcg ttggtgaaga gcgtggtctg attgctgaaa
     1741 tgatcgtcga tcttgtagtt atcggtcatt cccgcacgga cgctttcgat caccgtcttc
     1801 gatccgttga caagataggc cgggtcgctc caggcgatgc gatgataaat aatgctatcg
     1861 gtcatttcac cggccagctg ttcgagcgaa cgcgcataag cgaaaaaagc agagatcgtg
     1921 tttttgtcgc tcacgaaaaa atcgccgttg aattgagcgt agtccttccg cgacgcacta
     1981 ttcgtcctcc agccttcctc ttcctggtgc ccgtaattga aaagcaacga tgaatgttca
     2041 ttggccgatt cgatccgggt attgttgcgc cagagtccat agctcccacc cgtgttctct
     2101 tcgacgatac gggcctgacc cggttgcggt ttcagcgtga acagattgac gacgccggca
     2161 actccgctgc cgtaaaggct cgaggccggt cccttgatca cttccacttt ccccagcgtt
     2221 gaaaaatcga tatcgtccag gatggtcgtc cccgtggcgt cggtaacggg tatgttgttg
     2281 agcaggacct gcaccccctg accgttgaaa ttggtattat tgccataccc ccggatgatg
     2341 atacgttgtc ctccgaagct ggtgcgcgag cccatgctga cgccgggcat aaggttcagc
     2401 gaattttcca gcgaaaaccc ggtattgcgg tgaaaatctt cggccgtgag gatgccgaca
     2461 gaatgcgcta cgtccagctg gttgtccttt ttaccggtgg accagccggt cacctgcacg
     2521 gtattcagcg tttgcggttt caacgtatcg ttcgctgttt tgggagcgcg ttgagccgcg
     2581 gtggacaatg caataaatat aaacaataga aagacgcctg ttctttccaa catgatgtga
     2641 tctttaataa ataaaaatgg tattcctatc cgaaaagcca ggcgcttgct ttcggcggcg
     2701 gaatgacgat ccttgcatat cgggaactca ggcgatgaac gccataggtg ggatattgcc
     2761 tgtcgatggg ctgcaagggc gcgatggtcc attgcggcgt ttggcataac aggtcgcaga
     2821 tcgaaaaaga gatccttgtt tgtctgccgg gttgatcctg gttcgctttt gttacatcca
     2881 ccgctccatg gatcagctgc tcttctcctt cgtcgtcaag gcagtatagc cacgtggtgc
     2941 cactcaccgt ccgctgccgg atcacatcat acagatgacc cttataccag cactcccttc
     3001 cctcgtcttc ccatcgccct gacgcattca attccgcaac gctcacacgc agcattaccg
     3061 catcgggcaa cgccgcgatc cgtgcttcat tcgccgcttc tttcatccgc cattggagat
     3121 agatggtctg cccataaaag ccgaattggg tatagcatag gggaagaagc agcaatatgg
     3181 cgatcgatct cttcagacaa ctagtttttg ctgttgcgaa tatccaactt ttcgggcgga
     3241 agtccgcctc gtttgagacg cggaaaactt tgaaggaccg cttcatattt tttacgggcg
     3301 gcatccagcc ggccgctcag caacgcgtag acttccttgt gctggttcgt aggcctgaaa
     3361 tccgcaccgc cggaaatgct ttccttggag atcgtgagga accgctcgag cagttgtgcc
     3421 ggattacgga aaatatcctc cctggcgccg gtctgatgca cgtcgtgaag gagcgcctcc
     3481 gcttcataga gctggtcctt ccacgcctgc agcttcgcct tctgccggcg atcggtatag
     3541 agacgtccga tagagtcggc acggttgcga acgatctcga gcgtgtcgat catatgcagg
     3601 atgctttgga tcgaaccgta gatctgtacc ccgaaggcgt attgttcatg gatatcctcg
     3661 tccgaaccgc cggtatgcgg gtcccgaaac acctgcaccg gctgccggta ttcttttctg
     3721 cctatcttca gcacaacggt ataagtgccc ggcagaacct tggtggcagg cagtcccggg
     3781 ccgatatcca ggtcatagat caccatcgcc cgctgcccgg tcgaatcgag ctttacccag
     3841 tcggcatcct cggggcgggt tcgcaagggc ggtaatacat aaggctgcaa gccgagatcc
     3901 caccacaccc ggttgacgcc cttccggttt gatccggcta tctgttgcac ggtacgaccc
     3961 gcgctatcca ggatgagcac cttgacggtg tcccttacgg tatcgcgtaa ataatagttg
     4021 atatcggcgc cataaggcgg gttcaccccg aatgaagcgg agcgttcgcc atggatggcc
     4081 ggcttgggtc ggaaccgcca ggccttgcgc atcgggaaca accaggcttc cgattgccgc
     4141 accgaatcgc tgaaatccct gatcggggta atatcgtcga gaatataaaa accgcgtcca
     4201 taggtgccga tcaccaggtc tttgaaattc cgctggatcg ttaagccata gatcggcgcg
     4261 ggaggcaggt tattgcgaag atgtacccaa ctggcgccgt catcgggaga aaaatataaa
     4321 gcgtta
//
LOCUS       DASYSV010000233         4404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2347617, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000233 DASYSV010000000
VERSION     DASYSV010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2347617"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2406)
                     /locus_tag="VGQ51_07185"
     CDS             complement(<1..2406)
                     /locus_tag="VGQ51_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9036383.1"
                     /translation="MKIGILSIAFVLFLSTSFGQGKNNPGATGAIVGKVIDSASRLPL
                     ESATISLNATGKNRVLNRTTTDSSGNFGLTNILPGSYTVVIEYVGYRTINIRNIVVTQ
                     TRDIVNLKSIHAIPKTNVEQSVIVTAPPKLIDNKIDKLVFNAERDITSQTGVATDLLA
                     KVPQVSVDVDGNVELQGSSNILFLIDGKPSTIFGSNITDVLQAIPASQIKSIEVITNP
                     GAKYDANAAGGIINIILKHNTAQGVNGNLSLTAGTIIQNGSFNLNARKGKFGMNAFFD
                     GNARLSTTTPSSSLRTSTDTIAKTTTLLQQNGSSDFTRHGLQTGIGFDWSITDNNSLS
                     GGVSYHNFGNRANGYVDQLQRTQNVAGGPYTDTSSVNRISSGFTQYSIDPSLDFKRRF
                     AKKDQELEIEMDGSFAHPSSNTGNDQYAMPHDSLIYGTRNNNPAKENEYEVKADYTQP
                     LHDDVNLGVGGKFSGYDISTAANALVWNPYSGSYLYNAALSNNLDYHQRVYAGYAELN
                     FPIGKSIDARLGGRYERTQITSFYANAHQTIDNGYNTWIPSVFVLKKIGETQTIKLNF
                     TIRINRPDYGDLNPFINTSDPKNISTGNPSLKPEIWDRYEASYNKDLGKLGSFLVTMF
                     YRQSNGDIQRFIQYYPTLQVGDTTYTNVNLTTRQNIGIEENLGTNLFFDLHANDKFNV
                     RSNTTFFYRHTINQVDPGYNSSGTILRTNINASYQFAGGYTAEFFGNFSSHHHEAQGF
                     YPSFISYSLAIRKQFWNKKGSLALTANNFFSKYVDQRTQLYGPGFVTNSVRSIPYQSV
                     GINF"
     gene            2580..2810
                     /locus_tag="VGQ51_07190"
     CDS             2580..2810
                     /locus_tag="VGQ51_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEV9036384.1"
                     /translation="MEGGPLATTGDFVLLADEVNKVVKALETHGITVTAIHNHMLHDE
                     PRLFMLHFWGVGQPGNIAEGLKAALVETNSKL"
     gene            complement(2843..3226)
                     /locus_tag="VGQ51_07195"
     CDS             complement(2843..3226)
                     /locus_tag="VGQ51_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV9036385.1"
                     /translation="MNNETTIRARVENWARSVNDKNWDGILAWHHKDIVMYDVPPPFQ
                     SVGIDAYRQTWELFFSNSHSFHIVDLHVVAGEDVAFCYAPMKCIYREKLSEREVDLDF
                     RLTIGFKKIGGQWWFIHEHHSVPAE"
     gene            3342..4307
                     /locus_tag="VGQ51_07200"
     CDS             3342..4307
                     /locus_tag="VGQ51_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEV9036386.1"
                     /translation="MILIAIILLLSCQNGPDTTHLEKDLVHLGTKIPLSDVDGRIDHL
                     SYDQAGHRLFVAAFGNNTVEVVDLATRTRIHTITGLSEPQGVCYIPATGRLVVANGGN
                     GVCVFYDGTNYSEVGRVSLGGDADNVRLDRGRLYVGYGGGAIAVIDPAAMKVLGDLSL
                     KGHPESFQLNDSGRIFINVPDKNEIVVGVTGKVAATAEWKNKGASANFPMALDKKDGR
                     LFVVYRHPAQLRMLDCRSDAVLASSSCVGDADDVFYDARSGLIFVTGGDGYLDVFRGT
                     TLVNHLATRKGARTSLWLPDQRKLILAVPARGGEPAALWVYALHP"
BASE COUNT          964 a         1123 c         1201 g         1116 t
ORIGIN      
        1 gaaattgatc cccaccgatt ggtaaggaat gctcctgacg ctattggtca caaagccggg
       61 accatacaac tgggtacgct gatccacgta tttgctgaaa aagttgttgg ccgtcagcgc
      121 gagacttcct tttttgttcc agaattgttt gcggatcgcc aggctatatg aaataaagga
      181 gggatagaac ccctgcgcct cgtggtggtg cgaactgaaa ttcccgaaga actccgccgt
      241 atagccgccc gcgaattgat aagatgcatt gatattggtg cgaaggatcg taccgctcga
      301 gttgtatccg gggtccacct ggttgatcgt atgcctgtag aagaaggtgg tattggaccg
      361 gacattgaat ttatcgttcg catgcagatc gaaaaaaaga ttcgtgccga ggttctcttc
      421 gatgccgatg ttttgccggg tcgtcaggtt tacgttggtg taggtggtgt cacctacctg
      481 gagggttgga taatattgta tgaaacgctg gatgtcccca ttgctttgcc ggtaaaacat
      541 cgttaccagg aacgaaccca gcttgccgag gtccttatta taagaggctt cataccggtc
      601 ccagatctcg ggtttcagcg agggattgcc cgtactgatg tttttcggat cgctggtgtt
      661 gatgaatgga ttcagatccc cataatccgg tcggttgatc cgtatcgtaa aattcagctt
      721 gatggtttgt gtctcgccga tcttttttag cacgaaaacc gagggtatcc aggtgttgta
      781 gccgttatcg atcgtctgat gggcgtttgc gtagaacgaa gtgatctgtg ttcgctcata
      841 ccggccgccc agcctggcgt cgatcgattt cccgatcgga aagttgagtt cggcatatcc
      901 ggcatatacg cgctgatgat agtccaggtt attcgaaagg gccgcattgt acaaatagct
      961 gccggaataa ggattccaga cgagggcgtt cgccgcagtc gagatgtcat acccgctgaa
     1021 ttttccgccc actccgaggt tgacgtcatc gtgcagcggc tgggtgtagt ctgcctttac
     1081 ttcatactca ttctctttcg ccgggttatt gttccgtgtg ccatagatca gggagtcatg
     1141 cggcatagca tattgatcat taccggtatt gctcgaagga tgggcgaagc ttccatccat
     1201 ttcaatttcc agctcctggt cctttttcgc gaacctgcgt ttgaaatcca ggctcggatc
     1261 gatactatac tgggtgaagc cgctgctgat acgattgacg gagctcgtat ccgtatacgg
     1321 gccgccggct acattttgcg tccgttgcaa ttgatcgaca tagccgttgg cccggttgcc
     1381 gaaattgtga tagctcacac cgccggagag actgttgttg tcggtaatgc tccagtcgaa
     1441 cccgatgcct gtctgcaggc catgccgcgt gaagtcgctg cttccgttct gttgcaggag
     1501 ggtagtggtt ttcgctatcg tatcggtgga ggtccgaagg ctgctgctcg gagtggtggt
     1561 ggaaagtctt gcgttgccgt caaaaaaggc attcatcccg aactttcctt ttcgcgcatt
     1621 gagattgaaa gagccgttct gaataatggt tcccgccgtc agcgagagat ttccatttac
     1681 accctgcgcc gtattgtgct tcagaatgat attgatgatt ccccccgccg cattcgcgtc
     1741 gtatttggcg cccgggttgg tgatcacctc gatgcttttg atctgactgg cgggaatggc
     1801 ctgcagtacg tccgtgatat tgctgccgaa gatagtggaa ggtttgccgt cgatgagaaa
     1861 gagaatattg gagctgccct gcaattccac gttaccatcc acatctaccg acacctgcgg
     1921 gaccttcgcc agcagatcgg tagccacgcc agtctgcgag gtgatatctc tttccgcatt
     1981 gaataccagc ttgtcgatct tgttgtcgat cagttttggc ggcgccgtta cgatcacgga
     2041 ctgttcgaca ttggtcttcg ggatcgcatg gatgctcttc agattgacga tgtccctggt
     2101 ttgcgttacg actatatttc ggatattgat agtccgatat ccgacatatt cgatgacaac
     2161 ggtatagctg ccgggaagga tattggtcag cccgaagttg ccgctcgaat cggtcgtcgt
     2221 ccggttgagg accctgttct ttcccgttgc gtttaagctg atggtcgcag actccagcgg
     2281 gagacgggaa gccgaatcga tcactttgcc tacaatcgcg cctgttgcgc ccggattatt
     2341 cttcccttgt ccaaaagaag tagataaaaa aagaacgaat gcgatgctca gaattcctat
     2401 tttcatattc tcgggcaaag ataagttgta atgctgaagg aattccggag aaacccgcga
     2461 aggacagagg ctattcattc atacttaaat ctgctatatg attgtttgca aaagagttct
     2521 accgatcgtt gtagcatttt catccgtttt gagtgggatg gctcaggtgg ataccgcaaa
     2581 tggaaggagg cccgctcgcg acgacgggcg acttcgtttt gctggccgat gaggtgaaca
     2641 aggtagtgaa ggcattggag acgcatggca ttacggtgac ggccatccac aatcatatgc
     2701 tgcacgacga gccgcggctg ttcatgctgc atttttgggg agtgggccag ccgggaaaca
     2761 tcgctgaagg cctgaaggcc gcgctggtgg agacaaattc taagctatga tgcgcctgca
     2821 gcgtctttcc cgttcacgac ccttattccg ccggcaccga atgatgctca tgtatgaacc
     2881 accactgccc cccgatcttt ttaaacccga tggtcaaccg aaaatccaga tcgacctcgc
     2941 gctctgaaag cttttcgcga tagatgcact tcatcggagc ataacagaac gccacatctt
     3001 ctccggcgac gacatgcaga tcgacgatgt ggaacgaatg gctgttggaa aagaacagct
     3061 cccaggtctg gcgatacgca tcgatgccga cggattgaaa gggcggcgga acatcgtaca
     3121 taacgatatc tttgtgatgc caggcaagga tgccgtccca gtttttgtcg ttgacggacc
     3181 tggcccaatt ttcaacccgg gccctgattg tcgtttcgtt gttcataaca gtataaattt
     3241 tattgaacaa aaatacctta gcatcgttgg aataccgggg tgggaatgag acgaaacacg
     3301 ggatgttttg cgccaatccc cggcaaaaaa atatgcgaac catgatactc atagctatta
     3361 tcttattgct gtcctgtcag aatggacccg acacgacgca cctggaaaag gacctcgtac
     3421 atctcgggac aaagatcccg ctttcggatg tcgatggccg catcgaccat ctttcctatg
     3481 accaggcggg acaccggcta ttcgtagccg cgtttggcaa caataccgtg gaagtggtgg
     3541 atctggcgac ccgtacccgc atacatacga tcacggggct gagcgaacct cagggagtat
     3601 gttatatccc tgccaccgga cgactggtcg tcgcgaatgg cggcaatggc gtctgcgtct
     3661 tctatgacgg cacgaactat tccgaagtgg ggcgtgtatc gctgggcggg gatgccgaca
     3721 atgttcgcct ggatcgcgga cgcctgtacg tagggtacgg cggcggcgct atcgcggtga
     3781 tcgaccccgc agccatgaaa gtgctgggcg atctctccct gaaaggacat cccgaatcct
     3841 ttcagctgaa tgactccggc cgcatattca tcaatgtccc cgacaaaaac gagatcgtcg
     3901 ttggcgtcac gggcaaggtg gctgcgaccg cggaatggaa aaataaaggt gcctccgcca
     3961 atttcccgat ggctttggat aaaaaggacg gtcggctttt cgtggtatat cgtcatccgg
     4021 ctcaactgcg gatgctggat tgccgttccg acgccgtgct agcctcctct tcctgtgtcg
     4081 gcgatgcgga cgatgtgttt tatgacgccc gcagcggtct catctttgtc accggcggcg
     4141 atggctacct cgatgttttc cgcgggacca cgctggtcaa ccatcttgcc acccgaaaag
     4201 gcgcacgaac gagcctctgg ctgcccgatc agcgcaaact gatcctcgcc gtgcccgcca
     4261 gaggaggaga gccggcggca ttatgggtat atgcgcttca tccttgacta ttggccggcc
     4321 tgcatttcta tccttatcgc gtatacctga acgccacact cacgatcctc ccatccagcg
     4381 gcgcatacac ctcgttgaac accg
//
LOCUS       DASYSV010000234         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1576146, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000234 DASYSV010000000
VERSION     DASYSV010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576146"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VGQ51_07205"
     CDS             <1..257
                     /locus_tag="VGQ51_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV9036387.1"
                     /translation="SRHTPQKIDLEFVNRLREWQIPFSLVFTKADKENQRTVSANVKA
                     FLTSMRATWQFLPQHFVTSAVKKMGRDKILDLIGQMNEEE"
     gene            complement(371..844)
                     /locus_tag="VGQ51_07210"
     CDS             complement(371..844)
                     /locus_tag="VGQ51_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HEV9036388.1"
                     /translation="MLWRKFNGDVIELPIIEAVENAIRRETENGYRLKVCIGTDSQVK
                     GLETEFATVIVFLREGHGGFMFIHNEKTRHQYTIKERMLVEVAKSIEIAYELCNLFSK
                     YNVDMEVHADINTNPHFKSNDALKEAMGYILGMGFAFKAKPEAFASSSCANKVVN"
     gene            complement(898..1269)
                     /locus_tag="VGQ51_07215"
     CDS             complement(898..1269)
                     /locus_tag="VGQ51_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV9036389.1"
                     /translation="MPQEEKKTLVIGASANPGRYSNQAINRLRGKGHPVVAIGQRKVK
                     TGDVEIETEKMAFEGVDTVTLYLNPTRQKEYYDYILSLHPKRIIFNPGAENPELSGLA
                     RASGIQPLEACTLVLLSTGQY"
     gene            complement(1402..2217)
                     /locus_tag="VGQ51_07220"
     CDS             complement(1402..2217)
                     /locus_tag="VGQ51_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV9036390.1"
                     /translation="MKTTLFTVIAFITAAQFIPSQQAVAQGYTSEKINYLDAGGNSTH
                     EKKAVLIEQVVQFGDTIWEHNLYRVGRPMFRSARCSDPEGNVLNGHYITYNEVGIVDT
                     FGNYSKGKRTGSWHVYTPQGRLEQELFYRDGELLWTKDTVQLKHESDSMREVFKKDSA
                     GNPAGKTFTKVEIESEFPGGAAGWLRYLNKNLRYPDYAVKYKIMGMVVITFIVDNEGH
                     ISPSSIYLTRSVEFNIDHEATRIIFNSPDWTPAIQNGRKVKSYKKQPIVFRLS"
     gene            complement(2283..>2959)
                     /locus_tag="VGQ51_07225"
     CDS             complement(2283..>2959)
                     /locus_tag="VGQ51_07225"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HEV9036391.1"
                     /translation="AIDIGFEYLVKTQDVHIYGEPDDYYDYDWKIGAALLDIGVNQYQ
                     YGNQSRIAANPKNIVSDQELDEKFDYVQTVAGFNDSLATIMGSIRTPRGIFRIWNPAR
                     FELNIDRPLPEHFAVNADLTLNLGGNNMGKQLFTKQITLFALTPRWETRNLGGYLPVT
                     VTTDGKVWVGGAVKAGPFLLGVHNWGNVFSKQKIQNGGFYLALVIRPEDGFRLKEKKE
                     YTCPKD"
BASE COUNT          680 a          797 c          714 g          768 t
ORIGIN      
        1 acagccgtca taccccgcag aagatcgatc tggaattcgt caaccggctc cgagagtggc
       61 agatcccgtt ttcccttgtc ttcacaaagg ctgataagga gaaccaacga actgtcagcg
      121 ccaatgtaaa agcctttctc accagcatgc gcgccacctg gcaattcctg ccgcagcatt
      181 tcgttaccag tgccgtaaag aaaatgggca gggataagat cctggacctc atcgggcaga
      241 tgaatgagga ggaatagccg gcgccggcaa ccattcgcga tgaaaataca ggggagaagg
      301 aaagttttga gggagacgat ccctttgacc atggaggatc acggtcttaa cagtgacgga
      361 gagggtaggg tcagttgact accttattcg cacagctgct gctggcaaac gcttccggtt
      421 tggccttgaa ggcaaagccc ataccgagga tgtagcccat tgcttctttc agcgcatcat
      481 tgctcttgaa atgcgggttc gtgttgatgt ccgcatgcac ttccatgtcg acgttgtact
      541 tgctgaaaag attgcagagc tcataggcaa tctcgatgct ttttgcgact tctaccagca
      601 tcctttcctt gatcgtgtac tgatgcctgg tcttttcgtt gtgaatgaac atgaacccgc
      661 catgtccttc acgcagaaaa acgattaccg tagcaaattc cgtttccaaa ccttttacct
      721 gggagtcggt gccgatgcag accttcagcc gatagccgtt ttcggtctca cgccttatgg
      781 cgttttccac ggcttcaatg atgggaagtt cgatgacatc accattaaat tttcgccata
      841 gcataataaa tgatttttcg aatttaccga aaaacagcta accggcaaaa tcgaacctca
      901 gtattgcccc gtactcagca gtaccagcgt acaggcttcc agcggctgaa taccactggc
      961 acgcgcaagg ccggacagct cgggattctc ggcgccggga ttgaagatga tgcgtttggg
     1021 atgaagcgaa aggatataat cgtaatattc tttttgccgt gtcggattca aatataaggt
     1081 cacggtgtcg accccttcga aagccatttt ttccgtttcg atctcgacat ctccggtctt
     1141 cacctttcgt tgcccgatgg cgacgacagg atgacccttt ccgcgcagcc ggtttatcgc
     1201 ctgattgctg tatcgcccgg gattagccga agccccgatc accaatgtct ttttttcctc
     1261 ttgcggcata cgtatgaaac aattgggatg gcattttgtt caatagggca tgtatctgcg
     1321 ccgtggtccg gttgatgccc caattcgttc caaatcttcc ccaatctgct ataaacttcg
     1381 ctttcgcttc cggcgggctt gtcagctcaa acggaacacg atgggctgtt tcttatacga
     1441 tttgaccttt ctcccgtttt ggatcgccgg ggtccagtcg ggactattga aaatgatacg
     1501 cgtagcctcg tgatcgatgt taaactcaac cgacctggtt aagtagatgg aggagggtga
     1561 gatatgacct tcattatcca cgataaaagt gatgacgacc atacccatga tcttatattt
     1621 caccgcataa tccggataac gcaaattctt gttcagataa cgcaaccaac ctgcggctcc
     1681 gcccgggaac tccgactcga tctctacctt ggtaaaggtc ttaccagcag gattgccggc
     1741 actgtccttt ttaaacacct ccctcatact gtcggattcg tgtttcaact gtaccgtatc
     1801 cttagtccat agcagttccc cgtctctata gaacagctcc tgctccagcc ggccctgcgg
     1861 agtgtagaca tgccagctcc ctgtccgctt gcctttcgaa taattgccga aggtatcgac
     1921 gatccccact tcattatagg tgatataatg accgttcagg acattgccct ccgggtcact
     1981 gcatcgcgca gaccggaaca tcggtctgcc cacccggtaa aggttatgct cccaaatcgt
     2041 atcaccgaat tggacgacct gttcgatcag gacggctttt ttttcatgcg tggaattgcc
     2101 acctgcgtcg agatagttga ttttctcgct ggtatagccc tgcgccacgg cctgttggga
     2161 tggaatgaat tgagcggcag taatgaaggc gattacggta aacagggttg ttttcatgct
     2221 cgaatttgcc gcaaataaag aaaaaacgct ggcatctgca agggcgatcg ccctccggcc
     2281 gcctaatcct tcgggcaggt atattccttc ttctccttta accggaaccc atcttccggc
     2341 cggatcacca gcgccaggta aaaacctccg ttctggatct tctgtttcga aaagacattt
     2401 ccccaattat gcacgccgag caggaaagga ccggctttga cggcgccgcc gacccatact
     2461 tttccatccg tcgttacggt caccggcaaa tatcccccca gattccgggt ctcccaccga
     2521 ggggtcaacg cgaatagggt tatctgtttg gtaaacaact gtttacccat attgttgccg
     2581 cccaggttga gcgtcaggtc ggcattcacg gcaaaatgtt cgggcaatgg ccggtcgata
     2641 ttcagctcga accgcgccgg attccagatc cggaaaattc cgcgcggtgt acggatggag
     2701 cccataatcg tagcgagact atcattgaat ccggctacgg tctgtacata gtcgaatttt
     2761 tcgtcgagct cctggtccga cacgatgttc tttggattgg cggcgatccg gctttggttc
     2821 ccgtattggt actgatttac cccgatatcc agcagtgccg ctccgatctt ccagtcatag
     2881 tcataataat cgtcaggctc cccgtagatg tgtacgtcct gcgtcttgac aagatattcg
     2941 aagccgatat cgatagccg
//
LOCUS       DASYSV010000235        11304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_168112, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000235 DASYSV010000000
VERSION     DASYSV010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168112"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            389..1054
                     /locus_tag="VGQ51_07230"
     CDS             389..1054
                     /locus_tag="VGQ51_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV9036392.1"
                     /translation="MRPTRVEDTRRKREEDRKRTRQEIIRLADGLIRQQGFNAFSYAD
                     IAGTMEIRNAAIHYYFPTKSELGKAVIESELERIRQYQWRTSGNSGEEQVRFLVSVFY
                     RNSQLNNICLMGSLLPDFATFEPDLQNAVNRMCDTILEWMMASLDKARKEGKVRFEGE
                     PADRAALVVSTLLAALLLVRARGQSIFFHMVDRLLADLGVDWRVGDLPEVEWPFPESP
                     SFT"
     gene            1076..2317
                     /gene="fabF"
                     /locus_tag="VGQ51_07235"
     CDS             1076..2317
                     /gene="fabF"
                     /locus_tag="VGQ51_07235"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV9036393.1"
                     /translation="MNKVVVTGIGAITPLGNDPGTFWRQLVAGVSAAGPITHFDASRF
                     RTRFACELRNFSPGELLDRNELKRTDLFTQYALVAADQAIRDSGIDFSGMDPFDTGVI
                     WGSGQGGMETFEEQVKEYAAGDGTPRFSPFFVPKLIANMASGMISIKYGFMGINYTTV
                     SACSTSNTAFMDALTYIRLGKAKVIVAGGSEAPITAASFGGFTAMKAMSTRNDDPQHA
                     SRPFDLERDGFVMGEGAGALVLEEYEHARSRGAHIYAELAGAAMTADAYHMTHTHPEG
                     AGAFRAMSDALKDSGVGKDDVDYLNAHATSTPVGDLSETQAIGRLFGDTPPNLVISGT
                     KSMTGHLLGAAGAIEAIACLLAIRDGIIPPTINTRNLDPAIAPGLRIQLGQALERPVR
                     VAMSNTFGFGGHNGIVVFRKV"
     gene            2409..2822
                     /locus_tag="VGQ51_07240"
     CDS             2409..2822
                     /locus_tag="VGQ51_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEV9036394.1"
                     /translation="MKKLHIIILVLIAASVAVLISFMGDLSTYETIASARQKTGKTVT
                     IIAHLDKSQPLEYDPVRNPNFLAFHISDTLGNTAKVIYHFEKPYDLEKAERITLKGKM
                     VGDVFEITRKDGILLKCPSKYKDDPNYAQKALSQN"
     gene            2833..5295
                     /gene="ccsA"
                     /locus_tag="VGQ51_07245"
     CDS             2833..5295
                     /gene="ccsA"
                     /locus_tag="VGQ51_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEV9036395.1"
                     /translation="MQYIGEHLLPGKIGHFFALLSFAASFVAIIAYFRATNAKTPEEQ
                     TGWRKMGRMAFSIDVFAVFAVFFTIICIIASRLFEYNIAWEHSSRELSPQYLLACIWE
                     AQEGSFLLWNLWVCVMGLILIRKAGKWESPVMTVMSFTNFCIATMIMGIYVFGQKIGF
                     NPFILVRQTELGMSAPIFSQPDYMSIPQMQDGQGLNALLQNYWMVIHPPILFLGFSST
                     VVPFAYAIAGLWKKQYGDWTRAAVPWTLFSACVLGTGIMMGAAWAYEALSFGGYWAWD
                     PVENASLVPWLVMIAGLHCMVIYNATGHSLRPTFFFLITQFILVLYETFLTRSGILGD
                     LSVHAFVESGMNVQLGLFVVVFLVPAYWLFIARYRHIPRIAKEEATSSREFWMFIGSL
                     ILFLSSMYIIIFTSLPVINKIIDLFREKAKQFTTGDDQAFSYNRIEIFIAILLGLLTA
                     VTQYLKYKGTDNAVFLKKIVWPTAIALVVSVLISVFGGIHYDKYGIGFLSAIHLAMFG
                     AVYAIVANAAYIRAGLNGKLKAAGGSIAHVGFGMMMVGILLSSAKKQVLSYNTTGITL
                     NFSPESKQDPMENITLLKGTRTDMGKYWTTYVGNDSVDPASRTTYFKIHFEKKDGSEQ
                     FDLYPNFMKSTKGQMAPSPNPDKRHYLGKDIFTYISATNAAIKEDTAAFRSVVLGVKD
                     TAYYSKGFVILDSVEVDPNNDRFHFSRKDTALVAKLTVVGQDSMRYTAWPALYLKDNV
                     PHFLNDTVFAQDLALRFNMVAKDRKIDLGIKESSDMVPFVALKVLEFPQIGLLWLGTL
                     IMITGFVISILWRRRQARLSKG"
     gene            5406..6083
                     /locus_tag="VGQ51_07250"
     CDS             5406..6083
                     /locus_tag="VGQ51_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HEV9036396.1"
                     /translation="MRLPTLKYLLLFSVSLWGGVCAFGQTADTAKIRHIASDTSLPSN
                     IIRPIYGPNDTIIVPARIYGNELLPASSGEYVWVQAPMPPAMRRRYEEWTRLRNAVYV
                     TYPYARRAGMIMNEMNAKLALLTNEGKRKAYINSQEKVLKKEFTEPLEQLSIYQGKIL
                     MKLINRQTGNNCYNIIKEYKGGFTARFWQTVAFFFNSSLKQPYDPKGDDRAIESIVEE
                     VERMYHG"
     gene            complement(6151..6642)
                     /gene="rfaE2"
                     /locus_tag="VGQ51_07255"
     CDS             complement(6151..6642)
                     /gene="rfaE2"
                     /locus_tag="VGQ51_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEV9036397.1"
                     /translation="MKVPQIIQQKIFDLAGMQHQVRRWRLLGKKIAFTNGCFDILHQG
                     HIASLSDAAREADVLVVAVNSDASTRRLKGELRPVNGQDARAIVLASLLMVDAVIIFD
                     EDTPLRLIETLQPDVLVKGGDYTLDQIVGAKEVIAAGGRVVINPVLTGFSTTGLIEKI
                     HRL"
     gene            complement(6605..7660)
                     /locus_tag="VGQ51_07260"
     CDS             complement(6605..7660)
                     /locus_tag="VGQ51_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV9036398.1"
                     /translation="MKKRFLTALQYVLFSSIALFFLWLSVKDMDAKKWAQLKDALDRA
                     NYWLLIPVLGFLLFSHWLRALRWRQLIQPMGYNPSAVNCFLGVMIGYFVNLGPLRLGE
                     IVKCTILARYEKVPADKLVGTIVAERAFDVVCLLIVFGLTLFFQYDVIHTLTSGKTDA
                     LFHNASGQTSWSRIAIIVVIGAIFGAAFVWALTRFRHIGIVGKIRKIVLNIWHGLTSV
                     KGVRNKPLFFVYTAGIWGMYLLSTWVGFFAIDATRHLGIIDALSVLAMGSIAMIVSPG
                     GIGAYPLFIQKTVAFYGVAALPDGQALGWLLWFGQFLSFILFGTASFILLPRINRRPH
                     YEILTHESTADNTAKDI"
     gene            complement(7672..8016)
                     /gene="panD"
                     /locus_tag="VGQ51_07265"
     CDS             complement(7672..8016)
                     /gene="panD"
                     /locus_tag="VGQ51_07265"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEV9036399.1"
                     /translation="MDIEILKSKVHRATITEANLNYVGSLTLDEDLMDAANMIENEKV
                     QVVNVNNGARIETYLIKGKRGSGTCCLNGPAARTGTVGDVVIIISYAHMDFEEAKRFK
                     PGIVFPKEGNKM"
     gene            complement(8018..8881)
                     /gene="panC"
                     /locus_tag="VGQ51_07270"
     CDS             complement(8018..8881)
                     /gene="panC"
                     /locus_tag="VGQ51_07270"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEV9036400.1"
                     /translation="MILFKRAGDLAGWLQKQRAAGRRIGFVPTMGALHEGHISLITIS
                     KKNTSLTVSSIFVNPTQFNDPADYKKYPITIEKDIALLERAGADVLFLPDLSELYPAG
                     IKDLEHYDLGSLETLLEGKFRPGHFQGVCQVMRRLLNIVRPDDLFMGRKDYQQCMVIG
                     RLLELMGLPATLHRCPIVREADGLAMSSRNVRLDPEERFFAAGIHAALLTIREAWAAR
                     NAIGDILAKAIAILEENHFRIDYVSIADGTTLEPLTAWQEDAVALIAAFMGEVRLIDN
                     MNLSTFASSNK"
     gene            8993..9805
                     /locus_tag="VGQ51_07275"
     CDS             8993..9805
                     /locus_tag="VGQ51_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEV9036401.1"
                     /translation="MLQKKRILFIANEMSPYLELTEFAEIVNKLAIKSNDNGFEVRCI
                     MPRFGVVNERRHRLHEVVRLSGINVSVDNDDFPLQIKVASLPNARLQVYFLDNEDLFK
                     RKFIFHDENDKWFEDNGLRTVFYCKGALETVKKFGWPPDVIHCSGWMTGLIPLYLKTV
                     YKKEPVFAHSKCVFTIGQTTFKEKLGPDFQKLANIHPTIKEKDLEPYKDANNTAMFRG
                     GATYADAISFGAEKVDKKLVEEFGKVRGRKVLSFNAESDLTDYLQLYMDLSK"
     gene            9879..11132
                     /gene="metK"
                     /locus_tag="VGQ51_07280"
     CDS             9879..11132
                     /gene="metK"
                     /locus_tag="VGQ51_07280"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV9036402.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDHFLAFDPQSKVACETL
                     VTTGQVVLAGEVKSRAYLDVQDIARDVIRRIGYTKSEYMFEANSCGIFSAIHEQSSDI
                     NQGVDRKKKEEQGAGDQGMMFGYASNETEDYMPLALDLAHKILLELAALRRENKQIKY
                     LRPDAKSQVTLEYDDNNKPVRIDAIVVSTQHDDFDTEAKMQAKIKKDIIEILIPRVKA
                     KYKKYARLFNDQIKYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGK
                     DPSKVDRSAAYATRHIAKNLVAAGVADEVLVQVSYAIGVAQPTSINVNTYGTAKVKLT
                     DGQIAKKVTELFDMRPYFIENRLKLRNPIYSETAAYGHMGRQPQVVEKVFVSPDGKEV
                     KRKVELFTWEKLDYVPKVKKAFGLK"
BASE COUNT         2666 a         2897 c         3212 g         2529 t
ORIGIN      
        1 agggtaccgg tacgataacg agacgggcat cctcttccac gaaggggaga ccaaaaacgt
       61 tgttcttagg gttgctggga ccgttggagt taaaattcga taggtctgtc atgtcaggga
      121 gtatccgtat tgattaaaaa cgggacgcaa gttagttcag tataaagaca tagcaaaata
      181 gggggtccgg gcgccatgga aatgcggtat tggaggccgg gggcggggcg ggggtggctg
      241 gagccagcgc tggcgggggg caaggactgg gctgcccgga gcgtcgaggc cagggctgac
      301 gggtggctgg agccaggggc cagggttttt tcggggcggg tttgcttact tactagtaag
      361 taagtatttt tgctccgaaa agcgatatat gagaccaacg cgggtggagg ataccaggcg
      421 caagcgcgaa gaggatcgga aacggacccg ccaggagatc attcgtctgg cggatggcct
      481 gatccggcaa cagggcttca atgccttcag ctatgcagac attgccggaa caatggagat
      541 acgcaatgcg gcgatccact actattttcc tacgaaatct gaattgggaa aggcggtcat
      601 cgaaagcgag ctggagcgga tccgccagta ccagtggcgg acctcgggaa attccggcga
      661 agagcaggtc cggttcctgg tatccgtctt ctatcggaac agtcagctga acaatatctg
      721 tctcatgggc tcgcttcttc cggatttcgc gacctttgag cccgacttgc agaacgcggt
      781 caaccggatg tgcgatacga tcctcgaatg gatgatggca agcctcgata aggcgcgaaa
      841 ggaaggaaaa gttcgtttcg agggggagcc cgccgacagg gcggcattgg tcgtctcgac
      901 tttgcttgct gctctgttgc tggtacgcgc ccgggggcaa tccatctttt ttcatatggt
      961 ggatcggtta ctggcggact tgggggtcga ctggcgggtc ggcgatttgc cggaagtgga
     1021 atggccgttc cccgaatctc cttcattcac gtgaaaacga taacagacat atagtatgaa
     1081 caaagtagtc gtaacaggca tcggagctat caccccgttg ggaaacgatc caggaacttt
     1141 ttggcggcag ctggtcgccg gcgtcagcgc agccggaccc atcacacatt tcgatgcgag
     1201 caggttcagg acgcgttttg cctgcgagtt gcggaatttc agtcccggcg agcttctcga
     1261 caggaacgaa cttaagcgta ccgatctgtt tacgcaatat gcgctcgtgg cggccgacca
     1321 ggcgatccgg gattcgggca tcgatttttc cggtatggat cctttcgata ccggcgtcat
     1381 ctggggatcg ggacagggtg gaatggaaac cttcgaagag caggtgaagg aatatgccgc
     1441 aggagacggc acgccgcggt tcagcccatt tttcgtgccc aagctgatcg ccaatatggc
     1501 ttcgggcatg atctccatca aatacggttt catgggcatc aactatacga cggtgtccgc
     1561 atgcagcacg tcgaatactg cttttatgga tgcgttgacc tatatccggc tgggtaaagc
     1621 aaaggtcatc gtcgcgggag gatcggaggc gccgatcacg gcggcatctt ttggcggatt
     1681 cacggccatg aaggcgatgt cgacccgaaa tgacgatccg caacatgcct cgcggccttt
     1741 cgacctggaa agggacgggt tcgtcatggg agaaggcgcg ggagccctgg tgctcgagga
     1801 atatgaacat gccaggtcgc gcggggccca tatttacgcc gagctggccg gcgccgcgat
     1861 gaccgccgat gcatatcaca tgacgcatac gcatcccgaa ggggcgggcg cctttcgggc
     1921 catgtcggat gccctgaagg actcaggggt tgggaaggac gacgtagatt acctgaatgc
     1981 gcatgctact tcgactcctg tgggtgatct gagcgaaacg caggctatcg gacgtctatt
     2041 cggggacact ccgcccaatc tggtcatcag cgggaccaag tccatgacgg ggcatctgct
     2101 tggcgcggcg ggcgccatcg aggccattgc ctgtctgctg gccatccggg acggcatcat
     2161 tccgccgacg atcaacacca ggaacctcga tccggccatc gcaccgggtc tgaggatcca
     2221 gctgggtcag gcgctggaac gtccggtcag ggtcgcgatg agcaatacat tcgggttcgg
     2281 cgggcacaat gggatcgtgg tgttccggaa agtgtgaggg gcgggtacgg gcgagaagaa
     2341 aaccggcggg gccgtaaatg gaatttgccg tgggtgggaa ttgtttttgg agtaactttg
     2401 cggcagtcat gaaaaagctg catatcatta tcctcgttct catcgccgcg tccgttgccg
     2461 tcctcatcag cttcatgggc gacctctcca cgtatgagac catcgcttcg gcccggcaga
     2521 aaacgggcaa gaccgtcact atcatcgcgc acctggacaa atcccaaccc ctcgagtacg
     2581 atccggtcag gaatcccaat tttctcgcat tccatatcag cgataccttg ggcaataccg
     2641 ccaaagtgat ctatcatttc gaaaaacctt atgaccttga aaaggccgag cggatcacac
     2701 taaaaggaaa gatggtcggg gatgtcttcg agatcacccg caaagacggc atcctgctga
     2761 aatgcccttc caaatacaag gacgatccca attatgctca gaaagccctg agccaaaatt
     2821 aatgtacgta gcatgcaata tatcggcgaa catctgttac ccggaaagat aggccatttc
     2881 tttgcgctgc tttcctttgc cgcctctttc gtcgccatca tcgcctattt ccgggcgacc
     2941 aatgcgaaga cccccgagga acagaccgga tggcggaaaa tgggccggat ggccttttcg
     3001 atcgacgtgt tcgcggtttt tgccgttttc tttaccatca tctgcatcat cgcttcccgg
     3061 ctgttcgaat acaatatcgc ctgggagcat tccagccgtg agctgagtcc ccaatacctg
     3121 ctggcctgta tctgggaggc gcaggaaggc agctttttgt tatggaacct ttgggtatgt
     3181 gtcatggggc tgatcctgat ccgcaaggcc ggcaaatggg agtcgccggt gatgacggtg
     3241 atgagcttca ccaatttttg tatcgccacc atgatcatgg gcatctatgt cttcggccag
     3301 aagataggat tcaatccctt tatcctggtc cgccagacgg aattgggaat gagcgcgccg
     3361 atcttcagcc agcccgatta tatgagcatc ccgcaaatgc aggacggtca gggattgaat
     3421 gcgctgttgc agaattactg gatggtcatc cacccgccga tattgttcct gggtttttca
     3481 tcgacggtgg tccccttcgc atacgccatt gccgggctgt ggaaaaagca atacggggac
     3541 tggacccgcg cagcagtgcc ctggacactt ttttcggctt gtgtgctggg tacgggtatc
     3601 atgatgggcg cggcctgggc atacgaggcg ctgagcttcg gcggctattg ggcctgggac
     3661 ccggtggaga atgcttcgct ggtgccctgg ctggtgatga tcgcgggact tcattgcatg
     3721 gtcatctaca atgcgacagg tcattcgcta cgaccgacct tctttttcct gatcacccaa
     3781 ttcatactcg tgttgtatga aacattcctc acccgcagcg gcatactcgg cgatctctcg
     3841 gtacacgcct tcgtggagtc aggcatgaat gtacagctcg gtctgttcgt ggtggttttc
     3901 ctggtgccgg cctactggct tttcatcgcg agataccggc atataccgag gatcgcgaag
     3961 gaagaggcga cgagctcgag ggagttctgg atgttcatcg gatcgctgat cctgtttctt
     4021 tcgtccatgt acatcattat cttcacctcg ctgccggtga tcaataagat catcgatctg
     4081 tttcgcgaaa aggccaaaca attcactacc ggcgacgatc aggcgttttc gtacaaccgg
     4141 atcgagatct tcatcgcgat actcctcggc ctactgaccg ccgtgacaca atacctgaaa
     4201 tacaagggga cggacaatgc ggtctttctc aaaaagatcg tttggcctac cgcgatagcg
     4261 ctggtggtgt cggtgctgat cagcgtcttc gggggcatcc attacgataa atacggcatc
     4321 gggttcctgt ccgctataca tctggcgatg tttggcgcgg tgtatgcgat cgtggccaat
     4381 gcggcctata tccgcgctgg tctcaacggg aagctgaagg ccgccggcgg atcgatcgca
     4441 catgtcggat tcggcatgat gatggtgggg atactgctct cgtcggcgaa aaagcaggtg
     4501 ttatcctaca atacgaccgg gatcacattg aatttttccc ccgaatccaa acaggacccg
     4561 atggagaaca tcacgttgct gaagggcacc cgtacggata tgggaaaata ctggaccacc
     4621 tatgtaggca acgattcggt agacccggcc agcaggacca cctatttcaa gatacatttc
     4681 gagaaaaagg acggaagcga gcaattcgat ctgtatccga acttcatgaa gagcaccaag
     4741 ggccagatgg cgcccagtcc caatcctgac aaacggcatt atctgggcaa ggacatcttc
     4801 acctatatca gcgccaccaa tgccgccatc aaggaggata cggccgcctt ccgcagcgtc
     4861 gtgcttgggg tgaaggatac ggcctattat tccaaagggt ttgtgatcct ggatagcgtc
     4921 gaggtcgatc ccaacaacga tcgttttcat ttctcgcgga aggacacggc gctggtggca
     4981 aagctgacgg tggtcgggca ggacagcatg cgttatacgg cgtggcccgc gctctacctg
     5041 aaagacaacg tcccgcattt cctcaatgac acagtgttcg cgcaggacct tgcgctgcga
     5101 ttcaatatgg tcgcgaaaga ccggaaaata gatctgggca tcaaggagtc ttccgacatg
     5161 gttccgttcg tcgccctgaa ggtgctggaa ttcccgcaga tcgggttatt gtggctcggg
     5221 acattgatca tgattaccgg gtttgttatc agtatcttat ggagacgccg tcaggcgagg
     5281 ctttcgaaag gatagtccgc cgggggttac cttgggggtg tcgggggtat taactatggg
     5341 tgctccaaag ctgccgcccg cggcttgccc gacggatata aaattagtac ctttattgat
     5401 cgattatgcg tctgccgaca ctcaaatacc tgctcctgtt ctccgtatcg ttatggggcg
     5461 gcgtgtgtgc gttcgggcag actgccgata ccgcgaaaat ccggcatata gccagcgaca
     5521 cgagtcttcc ttccaacatc atccgcccga tctatgggcc caacgatacg atcatcgttc
     5581 ccgccaggat ctacggcaac gagctattac ccgcatcttc cggggaatat gtctgggtgc
     5641 aggcgccgat gcctccggct atgcgtcgtc gttatgagga atggacccgg ctgcgcaatg
     5701 ccgtttatgt gacctatccc tatgcgcgtc gtgccgggat gatcatgaac gagatgaacg
     5761 cgaagctggc cttgctgacg aacgagggaa agcgcaaggc ttatatcaac tcgcaggaaa
     5821 aggtgttaaa aaaggaattc accgagccgt tggaacagct atccatctac cagggtaaga
     5881 tcctgatgaa gctgatcaac aggcagacgg gcaacaactg ttacaatatc atcaaggaat
     5941 acaagggcgg tttcacggct cgtttctggc agaccgtcgc ctttttcttc aacagcagtc
     6001 tcaagcagcc gtatgaccca aagggggatg accgggcgat cgaatcgatc gtcgaggagg
     6061 tggagcgcat gtatcatggg taagtcgcgg gaggcgcgat cgccgggccg gcggtgatca
     6121 gaggaggcgc gatcgatggg ccggcggtgg tcacagccgg tgtatctttt cgatgagtcc
     6181 cgtggtggaa aatcccgtca gaacaggatt gatgacgacg cggccgccgg ccgcaatcac
     6241 ctcttttgcg cctacgatct ggtccagggt atagtccccg ccttttacca gcacatccgg
     6301 ctgcagggtt tcaatcagcc gcagtggcgt gtcttcatca aaaatgatca ccgcatccac
     6361 catcagcagc gaagcaagca cgatggcccg ggcgtcctgg ccgttgacag ggcggagctc
     6421 gcccttcaat ctccgggtag aagcatcgga attgaccgcc acgaccagca catctgcctc
     6481 ccgggccgca tcggataacg aggcgatatg cccctgatga aggatatcga aacaaccgtt
     6541 ggtgaaagca atctttttgc ccagcagccg ccagcggcgc acctggtgtt gcattccggc
     6601 gaggtcaaat atcttttgct gtattatctg cggtactttc atgtgtaaga atttcgtagt
     6661 gtggacgccg gttgatacgc ggcagcagga taaagctggc ggtgccgaaa agaataaagc
     6721 taaggaactg tccgaaccag agtagccagc cgagcgcctg gccgtcgggc agtgcggcta
     6781 caccatagaa ggccaccgtt ttctggatga acaggggata ggcgcctatg ccgccggggg
     6841 atacgatcat ggcgatgctg cccatcgcca gtacactcag cgcatcgatg atgccgagat
     6901 gacgggttgc atcgatggcg aaaaatccga cccaggtgct gagcaggtac ataccccaga
     6961 tgccggctgt ataaacgaag aaaagcggtt tgttgcgtac ccctttgacg ctggtcagcc
     7021 catgccagat attcaatacg atctttctga tctttcccac gatgccgatg tgcctgaaac
     7081 gggtaagagc ccatacgaaa gcggcgccaa agatggcgcc gatgaccact atgatggcaa
     7141 tcctgctcca ggaagtctgg ccgctggcat tgtggaaaag ggcgtctgtc ttgccggagg
     7201 tgagggtgtg gatgacgtca tattgaaaga agagggtcag accgaaaacg atcaacaggc
     7261 aaacgacgtc gaacgcacgt tctgcgacga tcgtcccaac caatttatcc gccgggactt
     7321 tttcataacg ggccaggatg gtgcatttaa cgatctcgcc gagacgcagc ggacccagat
     7381 ttacgaaata accgatcatt actcccagga aacaattcac cgccgaaggg ttgtatccca
     7441 tcggctggat cagctgtctc cagcgcagcg cgcgcagcca gtggctgaac aaaaggaaac
     7501 ccagcaccgg gatcaacagc cagtaattcg cccgatccag cgcatcctta agctgcgccc
     7561 atttctttgc atccatatcc ttgacgctca gccaaagaaa aaagagtgcg atcgaagaaa
     7621 agagtacata ttggagagcg gtcaggaacc tctttttcat agagccacaa gttacatttt
     7681 atttccttcc ttgggaaaaa cgatgcctgg tttgaagcgt ttggcttctt cgaaatccat
     7741 gtgtgcgtaa gaaataatga tgacgacatc gcccactgta cccgttcttg cagcgggacc
     7801 gttgagacaa caggtccccg agcctctttt gcctttgatg agataggtct ctatgcgggc
     7861 accgttattg acattcacta cctggacttt ttcgttctcg atcatattcg cggcatccat
     7921 caggtcttca tccagcgtca gactgcccac gtaattcaga ttggcttcgg tgatcgtggc
     7981 acggtggact ttggatttta gaatttcgat atccatgcta tttatttgag gacgcaaagg
     8041 tagacaaatt catattgtcg atgagcctga cctcgcccat gaaggcggcg atcagcgcga
     8101 cggcatcctc ctgccacgcg gtcagcggct ccagtgtcgt gccatcggcg atggaaacat
     8161 agtcgatgcg aaaatggttc tcttcaagga tggcgatggc tttggcgagg atatccccta
     8221 tggcgttgcg cgccgcccag gcttcgcgga tggtcagcag ggccgcgtgg atcccggcgg
     8281 cgaagaagcg ttcttccggg tcgagacgga cgttgcggct actcatggcg agcccgtcgg
     8341 cctcgcggac aatggggcaa cggtgcaatg tagcgggcag gcccatcagt tccagcagcc
     8401 gcccgatgac catgcattgc tggtaatcct tgcggcccat gaagagatcg tccggccgga
     8461 cgatattcag cagacgccgc atgacctgac agactccctg gaaatgaccg gggcggaatt
     8521 taccttccag gagggtctcg agcgaaccga ggtcatagtg ctcgaggtct ttgatcccgg
     8581 cgggatagag ctcgctgagg tcgggcagga aaaggacgtc ggcgcctgct cgttccagga
     8641 gtgcgatatc tttttcgatg gttataggat atttcttgta atcggccgga tcattgaact
     8701 gggtcgggtt gacgaatatg ctggaaaccg ttagtgaggt gttctttttt gatatggtaa
     8761 taagtgagat atgtccttca tgaagggctc ccatcgtggg tacgaagccg atgcgacgac
     8821 cggcagcccg ttgcttttgc aaccaaccgg caaggtcgcc tgcgcgcttg aataaaatca
     8881 ttttttgggc gttttaagcg tcaaaaccgg cgtctgtgcc gatttttcaa taaaaatcgt
     8941 acctttgcaa actcgtttag ttaaggttcg tctcaacaat ttttagaaaa taatgctaca
     9001 aaaaaagcga attttattta ttgccaacga aatgtcgcct tatttagagc tgacagaatt
     9061 tgcagagatc gttaacaagt tggcaattaa atcaaatgac aatggtttcg aggtgcgttg
     9121 tatcatgccg cgttttggag tggtaaatga gagaagacac agacttcatg aggtcgttcg
     9181 cctttcgggt atcaatgttt cggtggataa tgacgatttt ccccttcaga tcaaggtggc
     9241 ttcgctgccg aatgcacgtc ttcaggtgta tttcctggat aacgaagacc tgttcaagcg
     9301 caagttcatc tttcacgatg agaatgataa atggtttgag gacaatggtc ttagaaccgt
     9361 tttttactgc aaaggtgcgc tcgagaccgt gaagaagttc ggatggcccc cggacgtcat
     9421 ccattgcagc ggttggatga caggattgat ccccctgtat ctcaagactg tatacaaaaa
     9481 ggagccggtg ttcgcccata gcaagtgcgt gttcaccatc ggtcagacca ccttcaagga
     9541 aaagttgggg ccggacttcc agaaactggc caacatccat cctacgataa aagaaaagga
     9601 tctggagccg tataaggacg ccaacaatac cgcgatgttc cggggcggcg ctacctatgc
     9661 cgatgccatc agcttcggag ccgaaaaggt cgacaagaaa ctggtcgaag aattcgggaa
     9721 ggtcaggggc aggaaagtac tgtccttcaa cgccgaatcc gatttaacag attatttaca
     9781 gctgtatatg gatctcagca aataatctcc cggcataact tatctttgcg cgtcaatcag
     9841 gaataactca taaattctca aaaaaccctt tacaaaaaat gccttattta ttcacttccg
     9901 agagcgtaag cgaaggacat ccggataagg tcgccgacca gatctccgat gcgttgatcg
     9961 atcattttct ggcttttgac ccgcaatcca aggtagcctg tgaaacgctg gttacgaccg
    10021 gacaggtcgt actggccggt gaggtaaaaa gcagggcata tctcgacgtg caggacattg
    10081 cccgcgacgt cattcgccgg atcggttata cgaagagtga atatatgttc gaagcaaatt
    10141 cctgcggcat cttttccgcc atccatgagc agtcttccga tatcaatcag ggggtcgacc
    10201 ggaaaaagaa ggaagagcag ggagccggtg atcagggtat gatgttcggc tatgccagca
    10261 acgagaccga ggattatatg cccctggcgc tcgatctggc gcacaagatc ctgctcgagc
    10321 tggccgctct ccggcgcgag aacaagcaga tcaaatacct gcgccccgac gccaagagcc
    10381 aggtgacgct tgaatacgac gacaacaata agccggtgcg gatcgacgcg atcgtggtct
    10441 ccactcagca cgacgatttc gatacggaag ccaagatgca ggcaaagatc aagaaagata
    10501 tcatcgagat cctgatcccg cgggtgaagg cgaaatacaa gaaatacgcc cgccttttca
    10561 acgaccagat caaataccat atcaacccca ccgggaagtt cgttatcggc ggtccccacg
    10621 gtgataccgg tcttaccggc cgtaagatca tcgtcgacac gtatggcggc aagggcgcgc
    10681 atggcggcgg agcattcagt ggaaaggatc cctccaaggt agaccgttcg gcggcttatg
    10741 cgacccggca tatcgccaag aacctggttg ctgccggcgt tgcggatgaa gtgctggtac
    10801 aggtgtccta tgctatcggt gtggcacagc ccacgagcat taatgtaaat acctatggca
    10861 ccgccaaggt caagctcacc gacgggcaga tcgccaaaaa ggtcacggag ctgttcgata
    10921 tgcggcccta tttcatcgag aaccgtctca agctgcgtaa tcctatttac agcgagacgg
    10981 cggcctatgg tcatatggga cgccagccgc aggtggtcga gaaagtattc gtctctcccg
    11041 acggaaaaga ggtcaaacgc aaagtagagc tcttcacctg ggaaaaatta gattatgtgc
    11101 cgaaagtgaa aaaggctttc gggttgaaat aagataattc atcgacgaga aacctcccta
    11161 acgggaggtc ccctgaaccg ataggctcag gacattagaa aaaaagagac cggcgcgatt
    11221 ttttaccttg cgagccggtc tcttttttat ttttgcacca tggacaatcc ctggaagata
    11281 ctcgctgagc gacccatcta tgac
//
LOCUS       DASYSV010000236         7088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_43391, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000236 DASYSV010000000
VERSION     DASYSV010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43391"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VGQ51_07285"
     CDS             complement(<1..293)
                     /locus_tag="VGQ51_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036403.1"
                     /translation="MRKLLPFALVLCSGLPLFAIAAPRSDTTIYIQKDTPLSRYPSIR
                     LEWQLLINGATTQKGVIPILPLSSKHPTPASIPAKYPTGNAEVFLRLVYRPAAS"
     gene            398..1426
                     /locus_tag="VGQ51_07290"
     CDS             398..1426
                     /locus_tag="VGQ51_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV9036404.1"
                     /translation="MKKVSISDIARKAGVSVSTVSFVMNDKAVKMRISREVIEKVENV
                     AREMGYRPNQLARGLRTGKTKTIGLIVENISNAFFATLAKSIEDEAKRYDYKVVYCST
                     DNDEEKARDLINMLSQRQVDGYIITPTLNLAEDIRRLQAENKPVVLIDRYFPAHTEIP
                     AVLVDNYEGVARGMEYLMDKGYSRIALVTIETEMAHMKDRLRAYCDVLSRHSIPLQDS
                     LVQIVPYNSDHQAALNEVTSLLSGAGKSFDAVFFLTNYLGVLGIEAIKKLQIKVPGQL
                     AVLCFDDNDIFRLYTPSISVIRQPIEAIGQKAVSALMERLRHSGEETALPNAPVTLEA
                     DLVKRESI"
     gene            1538..2749
                     /gene="sucC"
                     /locus_tag="VGQ51_07295"
     CDS             1538..2749
                     /gene="sucC"
                     /locus_tag="VGQ51_07295"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEV9036405.1"
                     /translation="MNLHEYQAKELLKKYSVPVQEGIPVDNAGAAEEAYKHLKVQYGN
                     NFAVVKAQIHAGGRGKGKIQGTEQKGVAVGKSAEEIQQIASRILGGTLVTIQTGPAGK
                     KVNKVLIAQDVYYPGANPVKEFYLSVLLDRAKGQNVVMYSTEGGVDIEEVAHKTPERI
                     FKEWVHPGGGLQPFQARKIAFNLGLSGEAFKNCVRFVTNLYNAYVGLDCSMLEINPLF
                     KTSDEKIIAVDCKMNLDDNALMRHPELEALRDISEEDPTEVEAGKYNLNFVKLDGNVG
                     CMVNGAGLAMATMDMIKLSGGEPANFLDVGGTANATTVEAGFRIILKDPKVKAILINI
                     FGGIVRCDRVAQGVIDAYNSIGDIKVPIIVRLQGTNAEEAKQLITQSGLKVQSAILLS
                     EAAALVNKAVS"
     gene            2751..3575
                     /locus_tag="VGQ51_07300"
     CDS             2751..3575
                     /locus_tag="VGQ51_07300"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV9036406.1"
                     /translation="MSLTILQQIEETVRYIRTICPEKPAVGIILGSGLGNLSREVRVE
                     KEIAYTEIPHFPVSTVEGHQGKLIFGSIDGTPVVVLSGRFHYYEGYTLAQVVYPIRVL
                     KFLGVGTILLSNAAGGMRRDFRVGDLMIISDHISLFTVNPLVGANEDGLGPRFPDMSE
                     PYSRELIKKALVIAEGLKIPIHAGIYAGVTGPTFETRAEYKLLHIAGGDAVGMSTVQE
                     VIAARHLGMSVFAVSVITDLGIREEDNIITHEEVLAAASAAEPKLTALFKGMLKGL"
     gene            3633..5696
                     /locus_tag="VGQ51_07305"
     CDS             3633..5696
                     /locus_tag="VGQ51_07305"
                     /inference="COORDINATES: protein motif:HMM:NF025486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEV9036407.1"
                     /translation="MRRCRIILVLVALVSGEALLAQNPLGRFGNIGAGGGGGGKDSLV
                     HRKADTITINFRYLDSSRLQHLDSSVYYLGDKIPRPATWINMGNMGAASRNLVFTPRM
                     LSGWDPGWHAFDLYAFTAEGTRFFNTTKPYTELGYMLASRSEQDIDVVHTQNVRPNWN
                     FGLEYRLINAPGTFINQNSNHNNYRFSSWYQSRNKRYQNFFVLVSSKLTASENGGIQD
                     PKALDSTKYSTQATIPVQIGPNLAAIYSNPFISTVTTGTKYSTSTYLMRQQYDLGQKD
                     SIVTDSSVIPLFYPRLRIENTEAFTTYNYKFIDYYYPGSYNLDTGYYTHHLGLGYIQP
                     TDTVTRQDNWHVLSSDMSLYQFPDAKNPQQFFKLGATLEVLSGRFDTSALVTGNLLTA
                     HKVHDQNVFGHAEYRNKTRNQKWDIEAYGRLYLNGLDVGDYNAYIALRRLISRNLGYF
                     EAGFENTNRTPGFIFDRASSFNLDTAAQRRNFLKENTTHLFASIDQPKHQFNLSASLY
                     LLSNYTYVADYYKEAQASSLFNLLQVSLRKQFTIYRHWHWRTLTILQQVAGSSPVHVP
                     LLVSSNQVGYDGSLGFKNLNLSFGAEIRYISPYKADGYSPVTGQFFSQKATNTTITQN
                     LPDINLYLHLRIRGFTAYVQGENLNAMAFHPAGFGFYNNNFVAPYYPEPGFVFRFGIF
                     WSFIN"
     gene            complement(5749..6699)
                     /locus_tag="VGQ51_07310"
     CDS             complement(5749..6699)
                     /locus_tag="VGQ51_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV9036408.1"
                     /translation="MAHTHTHDHSHSHGHSHAHGDHHHAVNLTSVNRAFIFGIVLNFL
                     FVIIEVVVGLSIRSLSLLSDAGHNLADVASLAMALIAIRLLKIKPNDKFTYGFKKTTI
                     LVALLNAAILLVSIGAIGYEALHRLMHPEPLPGKTISIVAAIGIVINTLTALLFLRDK
                     DNDLNIRGAFLHLLSDAIVSAALVIGGIAIFYTHLFWIDSVLSLLVAIVILISTWQLL
                     RDSLRLSLDAVPAGIEIKKVKEVVSKIGGVRGFHHIHIWAISTTENALTAHLVVEPSS
                     NMEFIEKLKHTIKHELLHQNIQHATLEVETEDAPCDEPDC"
     gene            complement(6712..>7088)
                     /locus_tag="VGQ51_07315"
     CDS             complement(6712..>7088)
                     /locus_tag="VGQ51_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV9036409.1"
                     /translation="APAPLIHLSTLFIVPSGKLAMKYLKDIPVKELRPGFFGKMVHGD
                     KSSLIFWDIKKGSALPEHHHVHEQITFIAEGQLEMVIGGEKYLLTKGSVYVIPSNVPH
                     SAFAVTDCRVIDSFAPARDDYR"
BASE COUNT         1683 a         1679 c         2124 g         1602 t
ORIGIN      
        1 gaagcggccg gtcggtatac aaggcggagg aagacttctg cgttgccggt ggggtattta
       61 gccgggatgg acgccggggt ggggtgtttg gaggagagag gcaatatggg gataactcct
      121 ttttgcgtgg tggcgccatt gatcaatagc tgccattcga gacgtatgga cggatagcgg
      181 ctgagggggg tgtccttttg gatatatata gtggtatcgg agcggggggc ggcgatggcg
      241 aaaaggggca accctgagca aagtaccagc gcgaagggca gtagctttct cataaaacag
      301 agcctttggt aatataataa taagcaaaag aaataagaaa gatgggctaa aacgatttag
      361 tacttttgat taactttaca tccaattccg atattacatg aagaaagtct ctatcagcga
      421 tatcgcccgc aaggcaggag tatcggtctc cacggttagt ttcgtgatga acgacaaagc
      481 agtgaaaatg cgcataagcc gcgaagtgat agaaaaagtc gagaacgtcg cccgcgagat
      541 gggttatcgt ccgaaccaac tggcgcgggg attgcggaca ggcaagacca agacgatcgg
      601 tctgatcgtg gaaaatattt ccaacgcttt tttcgccacg ctggccaaat ccatcgaaga
      661 tgaagccaaa cgttacgact acaaggtagt ttattgcagc acggataacg acgaggagaa
      721 ggcgcgcgat cttatcaata tgctgtcgca acgacaggtc gacggctata tcatcacacc
      781 tacgctgaac ctcgcggaag acatccgccg gttgcaggcc gagaacaagc ctgtcgtgct
      841 catcgaccga tattttccgg cgcataccga gatccctgcc gtgctagtcg acaactatga
      901 gggcgtcgcg cgtggcatgg aatacctgat ggataagggc tattcgcgga tagcgctggt
      961 gacgatcgaa accgagatgg cgcatatgaa ggatcgtcta cgcgcgtatt gcgacgtgct
     1021 gagccgtcat agcatcccac tacaggacag tcttgtccag atagttccat acaacagcga
     1081 tcaccaggcc gcgttgaatg aggtgaccag tcttttgtcg ggggcgggaa agtccttcga
     1141 tgccgttttc tttttgacca actatctcgg cgtgctgggt atcgaggcca tcaagaaatt
     1201 gcagatcaaa gtgcccgggc agctagcggt actttgcttc gacgacaacg atattttccg
     1261 gctatatact cccagcatat cggtgatccg tcagcctatc gaagccatcg ggcagaaggc
     1321 ggtatccgca ctgatggagc gtttgcgtca ttcgggagag gagaccgcgt tgccgaacgc
     1381 gcccgtcaca ttggaggccg atctggtcaa acgcgaatcg atatagattt tttcctattt
     1441 tcgcacttcc ttgcccgaag ggcagacatt agcgctttgc ggaaaatgcg ctaatttcgt
     1501 tgaaagcgta ttttccgcac ataaaatttt tccgctcatg aatctgcatg aatatcaggc
     1561 caaagaacta ctgaagaaat attcggttcc cgtccaggaa ggcattccgg tcgataatgc
     1621 gggagccgcc gaagaagcat acaagcatct caaggtccaa tatggaaata acttcgcggt
     1681 ggtaaaggcg cagatccatg caggaggaag gggaaaaggg aagatacagg ggacggagca
     1741 gaagggtgta gcggtaggaa agagcgccga ggaaatacag cagatcgcat cgcggatcct
     1801 ggggggcacc ctggtgacca ttcagaccgg gcccgcgggc aagaaagtga acaaggtgtt
     1861 gattgcgcag gatgtctatt atcccggcgc caatcccgtc aaggaatttt atctgtcggt
     1921 cctgctggac agggcaaagg ggcagaatgt ggtcatgtac agcaccgaag gtggtgtcga
     1981 tatcgaggaa gttgcgcata agacgccgga gaggatcttc aaggaatggg ttcatcccgg
     2041 cggcggtctt cagcccttcc aggcgcgcaa gatcgcattc aacctgggac ttagcggcga
     2101 ggcattcaag aattgcgtcc ggttcgtcac caatctctac aatgcctatg tggggctgga
     2161 ctgttcgatg ctcgagatca atccgctgtt caagaccagt gatgaaaaga tcattgccgt
     2221 cgactgcaag atgaatttgg atgataacgc gctgatgcgt catcccgaac tggaggccct
     2281 gcgcgatatc agcgaggagg accctacgga agtcgaggct ggcaaataca atctgaactt
     2341 cgtcaaattg gatgggaatg taggatgcat ggtcaatggc gcgggtctgg ccatggccac
     2401 catggatatg atcaaactga gcggcggtga gcctgccaat ttcctggatg tcggcggtac
     2461 cgccaatgca acgacggtag aggccgggtt ccgtatcatc ctgaaagatc ccaaagtaaa
     2521 ggcgatactc attaacatct tcggcggtat agtgcgctgc gaccgggtgg cgcagggagt
     2581 tatcgatgca tacaactcta tcggggacat caaagtaccg atcatcgtcc ggttacaggg
     2641 cacgaatgcg gaggaagcca agcagctgat cacccaaagt ggtttgaaag ttcagtctgc
     2701 gatcctgttg agcgaagcgg cggcactggt caacaaagcc gtcagctgat atgtctctca
     2761 ccatcctgca gcaaatagaa gaaacggtca ggtatatccg gacgatctgt ccggaaaagc
     2821 cggcggtggg gatcatcctg ggcagcggcc tgggcaatct ttcccgtgag gtcagggttg
     2881 aaaaggagat cgcttatacc gagatcccgc atttcccggt ctcgacggtg gaaggccacc
     2941 agggtaagct gatcttcggt tccatcgacg ggacgccggt ggtagtcctt tccgggagat
     3001 tccattacta tgaagggtat acgctcgcgc aggtggtcta cccgatcagg gtgttgaaat
     3061 tcctcggtgt cgggaccatc ctgctgtcga atgccgcggg gggtatgagg cgtgattttc
     3121 gtgtcggcga tctgatgatc atcagcgatc atatttcgct gttcaccgtt aacccgctgg
     3181 tgggggccaa tgaagacgga ctggggccga ggttcccgga tatgagcgaa ccttatagcc
     3241 gtgagctgat caaaaaggcg ctggtcatcg ccgaaggcct gaagataccg atccatgcag
     3301 gcatttatgc gggagttacg gggccgacat tcgagacccg cgcagaatac aagctattgc
     3361 atatcgccgg tggcgatgcg gtagggatga gcaccgtgca ggaagtgatc gcggcgagac
     3421 atctggggat gtcggtcttt gccgtcagcg tgataaccga cctgggtatc agggaagagg
     3481 ataatatcat cactcatgaa gaagtgctgg ctgcggcgtc ggccgcggaa cccaagctga
     3541 cggcattgtt caagggtatg ctcaaagggc tatgatggcc gatgggtttt aactttttgt
     3601 tctccactta atgagtaact tgaatttcat cagtgaggcg ttgtagaatc atattggtgc
     3661 tggtggccct ggtctccggt gaggccctgt tggcgcaaaa tccgttgggc cggtttggca
     3721 atatcggggc cggcggaggg ggagggggga aagactcgct ggtgcatcgg aaagcggata
     3781 ccatcaccat caacttccgg tacctggaca gcagcagact acaacacctg gattcatccg
     3841 tctattacct cggggacaag atacctcgcc ccgctacctg gatcaatatg ggcaatatgg
     3901 gagcggcgtc ccgtaacctg gtatttacac cgcggatgct ttctggttgg gaccccggtt
     3961 ggcatgcctt cgatctttat gcctttaccg ccgaggggac gaggtttttt aacaccacca
     4021 agccatatac ggaattggga tatatgctgg ccagccggtc ggagcaggat atcgatgtcg
     4081 tgcatacgca gaatgtcagg ccgaactgga atttcgggct ggagtacagg ctgatcaatg
     4141 cgcccggtac ctttatcaac cagaattcca atcacaataa ctaccggttc agctcctggt
     4201 atcaatcgag gaacaagcgg tatcagaact ttttcgtgct ggtgtcgagc aagctgaccg
     4261 cgtccgagaa cggcgggata caggacccga aggcactgga ttcgaccaag tacagcacgc
     4321 aggcaacgat acccgtacag atcggtccca acctggcggc catctatagc aacccgttca
     4381 tttccacggt cactaccggc acgaaatact cgacctcgac ctatcttatg cgtcagcaat
     4441 acgacctggg tcagaaagat tcgatcgtga cggactcatc cgtgatcccg ctgttctatc
     4501 cccggcttcg gatcgagaat acggaagcgt ttaccacgta caactataag ttcatcgatt
     4561 attattatcc ggggagctat aatctggata ccggttatta cacgcatcat ctcggtcttg
     4621 ggtatatcca gccgaccgat acggtcacgc ggcaggacaa ctggcatgtg ttgtcgagcg
     4681 atatgagcct gtatcaattc cccgatgcga agaatccgca gcagttcttc aagctggggg
     4741 ctacgctgga agtgctgagt ggccggttcg atacgtcggc gctcgtgacc ggcaacctgc
     4801 tgacggcgca caaggtccac gatcagaacg tcttcgggca tgccgaatac cgtaataaga
     4861 cccgcaatca gaaatgggat atcgaagcct atggaaggtt gtatctgaac ggcctggacg
     4921 tcggggatta caatgcctat atcgcgctga gaaggttgat cagccggaac ctgggttatt
     4981 tcgaagccgg tttcgagaat acgaaccgga cacccggatt tattttcgac agggcgagca
     5041 gtttcaacct ggacaccgcg gcgcagcgcc ggaatttcct caaggagaac acgacccatc
     5101 tttttgcctc gatcgaccag ccgaagcatc aattcaatct ttctgcgtcg ctttacctgc
     5161 tgagcaacta tacatatgta gcggattatt ataaagaggc gcaggcctca tcgctgttca
     5221 acctgttgca ggtgtcgttg cggaaacagt ttacgatcta ccggcactgg cattggcgga
     5281 cgctcacgat actgcagcag gtggcgggtt cttcgccggt gcatgttccc ctgttggtgt
     5341 cgagcaacca ggtcggatat gacggaagtt tgggtttcaa gaatctgaac cttagtttcg
     5401 gggcggagat taggtatatc agtccttata aagccgatgg gtattcgccg gtgacggggc
     5461 agttcttttc gcagaaggcc accaatacga cgatcactca aaacctgccc gatatcaatc
     5521 tttatctgca cctgaggata cgcggattca cggcgtatgt acagggagag aatctcaacg
     5581 cgatggcgtt tcatccggcg ggattcgggt tttataataa caatttcgtg gcgccgtatt
     5641 atccggagcc gggtttcgtt ttccggttcg ggatattctg gtcgttcatc aattagctcg
     5701 caggcataat gtctttttca atcatgggcg gagcggggtc ggcggcgctc agcaatccgg
     5761 ctcgtcgcag ggtgcgtctt cggtttcgac ctcgagggtg gcgtgctgta tattctgatg
     5821 cagcagctcg tgtttgatcg tgtgtttcag cttttcgatg aattccatat tggagctggg
     5881 ctcgacgacg aggtgggcgg tgagggcatt ttccgtggtg ctgatggccc agatatggat
     5941 atggtggaag ccgcgtacgc cgccaatctt gctaacgacc tctttgacct tttttatctc
     6001 gatgccggcg gggacggcat ccagggaaag gcgcaggcta tcccgaagca gctgccaggt
     6061 gctgatcagg atgacgatcg cgacgagcag gctgagcacg gagtctatcc agaacagatg
     6121 ggtataaaag atggcgatac cgccgatgac gagcgctgcc gatacgatgg cgtcggacag
     6181 caggtggagg aaggcgccgc ggatgttgag atcgttgtcc ttatcgcgca ggaaaagcag
     6241 ggcggtcagc gtattgatga cgatgccgat ggcggctacg atagatatgg tcttcccggg
     6301 aagcggttcg gggtgcatca ggcggtggag cgcttcatag ccaatggcgc cgatggagac
     6361 gaggaggatg gcggcattca ggagggcgac gaggatcgtg gtctttttga agccgtaggt
     6421 gaatttgtcg tttggtttta tttttaacag acggatagca atgagggcca tggcgagaga
     6481 ggccacgtcg gccaggttgt gaccggcgtc ggataaaagc gataacgagc ggatggaaag
     6541 gcccacgacg acttcgatga tgacgaaaag aaaattcagg acgatgccga agatgaatgc
     6601 cctgttcaca cttgtcaggt tgactgcgtg atggtgatca ccgtgggcgt gtgagtgacc
     6661 gtgggaatgg gagtgatcgt gtgtgtgcgt gtgtgccata gggtggtgtt tttaccgata
     6721 gtcatcccgt gccggcgcga aggagtcgat gacccggcaa tccgtcacgg cgaatgccga
     6781 atgggggaca ttggacggga taacgtagac ggagcctttg gtgagcaggt atttttctcc
     6841 tccgatgacc atttccagct ggccttcggc gataaaagtg atctgttcgt ggacgtgatg
     6901 gtgttccgga agggcgctgc cttttttgat atcccaaaat atgagggagc ttttgtcgcc
     6961 gtggaccatc ttcccgaaaa aaccgggccg caattctttt accgggatgt ccttcaggta
     7021 tttcatagcg agttttcccg aaggtacaat aaataatgtg gataagtgga taagcggcgc
     7081 tggtgcgg
//
LOCUS       DASYSV010000237         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2089766, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000237 DASYSV010000000
VERSION     DASYSV010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089766"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VGQ51_07320"
     CDS             complement(<1..819)
                     /locus_tag="VGQ51_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036410.1"
                     /translation="MTEKHLAKSAALMAVLVVLALGTWEFSLRKKGIGIAYDENAALW
                     ADKRAMVYEPADQATVFIGSSRIKYDLDIPTWERHTGKHAIQLALQGNSPLPVLEDLG
                     NDPKFKGRVVVSVMEMLYFSLAPPVTEEIKGYVHYYHAETPSQKASFRVDRVLESRFV
                     FLDQDFLTINAGLDNLRIPNRQGVFPGLLFPLDFSRTGFDRQTKMTDHFVEDTSLQAR
                     VEQQWILAGNMMKNAPPSKEDPVAIVMATTKDAVAKIRARGGDVIFVRPPSSGPM"
     gene            complement(1194..2981)
                     /locus_tag="VGQ51_07325"
     CDS             complement(1194..2981)
                     /locus_tag="VGQ51_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEV9036411.1"
                     /translation="MIKLILSFFFWASLAQTYGQPASSWIRINQMGYPPAGVKVAVLA
                     SHSRIMSDEFQVVSVTTGKSVFEGHASASFGAYGPFADTRRLDFSSFNKEGAYFLRAG
                     SATSPIFSISGGVYDGAADFCLRYMRQQRSGFNPFLGDSCHTHDGYTLYAPVPDSTHI
                     DVVGGWHDASDYLQYSATTANAVYHLLAAWRDFPRVFGDRYLADGLPGPNREPDVLDE
                     ARWGLNWMLKMHPKPGWLFNQVGDDRDHRGMRIPKEDTNFYGRGLERPVYFCTGAPQV
                     RGKFMNNSTGVASTAGKFAGAFALGARIFGGDEAGAGVDRSYAALLREKSVTALQLGL
                     DKPGVCQTASVLSPYIYAEDNWTDDMELAAASVGQLDLAYHYALAEPVTPWLGHDTAN
                     HYQWYPFINIGHYELARRLHDRRRDSIIAFYKEGISRVWQRASANAFYRGIPFIWCSN
                     NLTVAFAIQCYWYRQLSGDKTFLPLEQACFDWLFGCNPWGKSMVYGLPAGGDTPKDPH
                     SAFTHIGYYAIDGGLVDGPVYTSIYSHLIGIHLSHEDAYAAFQSGLAVYHDDYGDYST
                     NEPTMDGTASLVYLLAAREAEAKNSGFRK"
     gene            3025..>3243
                     /locus_tag="VGQ51_07330"
     CDS             3025..>3243
                     /locus_tag="VGQ51_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEV9036412.1"
                     /translation="MSKILLLFFLTGISSVSKAQPYALLIRGGYVIDPKTAVRDTLDI
                     AVQKGCIVRMAKRIDPRQAARIVDARGAF"
BASE COUNT          761 a          889 c          866 g          727 t
ORIGIN      
        1 catgggaccg ctggatggcg gccttacaaa aattacgtct cctcccctgg cccggatctt
       61 tgcgacagca tcctttgtcg tcgccatgac gatggcgacg gggtcctcct tcgacggcgg
      121 cgcgtttttc atcatgttgc cggcgaggat ccattgttgc tcgacccttg cctgaaggct
      181 ggtatcttcc acaaaatgat ccgtcatctt cgtctggcgg tcaaaaccgg tccttgaaaa
      241 atccagcggg aaaagcaggc cggggaagac accttggcgg ttgggtatcc tcaggttatc
      301 cagccccgcg ttgatcgtca ggaagtcctg gtccaggaaa acgaaccgcg attccaatac
      361 ccggtcgacc cggaagctgg ctttttgaga gggggtttcc gcatgataat agtgaacgta
      421 gcctttgatc tcttccgtca ctggcggcgc caggctgaag taaagcatct ccattacgga
      481 tacgaccacc ctccccttga atttcggatc gtttcccagg tcttccagga cgggtaaagg
      541 tgaattgccc tgaagcgcaa gctggatcgc gtgctttccc gtatgacgtt cccacgtagg
      601 gatgtccagg tcgtatttga tacgcgagct accgatgaaa acggtcgcct gatccgccgg
      661 ttcatagacc atcgcgcgtt tgtccgccca tagcgcggcg ttttcgtcat aggcgatccc
      721 gatgcctttt tttcgaagcg agaattccca ggtgccgagg gccaggacaa ccaataccgc
      781 catcaatgcg gctgactttg ccaggtgttt ttctgtcatt ttaagggtta tattaggagt
      841 taagagaaat aagcagattg gttcgcacga ttcatgcgaa cccgcgacta atagggagaa
      901 atgtggtggt attcgatttg tgagaggtgg agacgatcag gtggataacg ctattgccaa
      961 tatagggtat ttgcgggatt cccgcggctt ccgcgcgatt tttacccaat taccggtata
     1021 gtaggatctt aggcgatcat ggactcccct ttgtttagat cgtccttatt ttgcagtgcg
     1081 aagttgatgg cagtcttgat atattgcccg gatggggttg tgcaagatga ttacttgttg
     1141 ttgggcgttc gcaaattttc tgcccgaccc tccggtttat ctttcattcg cctttatttt
     1201 cgaaaaccag aatttttcgc ctccgcctcc cgcgccgcca gcaaatacac cagcgaagcc
     1261 gtcccatcca tcgtcggctc attcgtgcta taatccccat aatcatcatg atagaccgcc
     1321 aggccactct gaaatgccgc atacgcatcc tcatgcgaaa gatggatccc aatgagatgc
     1381 gaatatatac tcgtatacac cgggccatcg acgagaccgc cgtcgatagc ataatacccg
     1441 atatgcgtaa aggcggaatg agggtccttc ggcgtatcgc cacctgcagg caggccatag
     1501 accatactct tcccccacgg attgcacccg aatagccagt cgaaacaggc ctgttcgaga
     1561 ggcaaaaacg tcttgtcgcc gctgagctgt cggtaccagt agcactggat ggcaaaggcg
     1621 acggtgagat tgttgctgca ccagataaaa ggaatcccgc gataaaaggc gttagccgaa
     1681 gcgcgctgcc aaaccctcga gatgccctcc ttataaaaag cgatgatgga atcacgacgc
     1741 cggtcatgca gacgcctggc gagttcataa tgtccgatat tgataaaagg ataccattga
     1801 tagtggttcg cagtatcgtg tcccagccag ggtgtgacag gctcggccag cgcataatga
     1861 taggcgagat cgagctgtcc gaccgaagcc gccgccagtt ccatgtcgtc cgtccagttg
     1921 tcctcggcat agatgtaggg cgaaaggacc gaagcggtct gacatacacc gggtttatcg
     1981 agaccaagct gcagggcggt gactgatttt tcccggagta acgcggcata gctgcgatcg
     2041 acaccggcgc cggcctcatc gcccccgaat atgcgcgcac caagcgcaaa cgctcccgcg
     2101 aatttaccgg ccgttgaagc cacccccgta ctgttattca taaattttcc tctcacttgc
     2161 ggtgcacctg tacaaaaata gactggccgc tccagccccc gcccatagaa attggtatcc
     2221 tctttcggaa tacgcatacc ccgatgatcc ctgtcgtcgc ctacctggtt gaacagccag
     2281 cccggcttag gatgcatctt cagcatccaa ttgagccccc accgcgcctc gtcgagcaca
     2341 tcaggctcgc ggttcggccc cggcaaacca tcggccaggt atcgatcccc gaagacccgt
     2401 ggaaaatccc tccaggcagc cagcaaatga tagacggcat tggccgtagt tgcggaatat
     2461 tgaagataat cgcttgcatc atgccagccg ccgaccacgt cgatatgggt actgtctggc
     2521 accggcgcgt atagcgtata accatcgtgc gtatgacagg aatcgcccaa aaaaggattg
     2581 aagccgctgc gttgctgccg catataacgc agacaaaaat cggccgctcc gtcatagacc
     2641 cctccactga tggagaagat aggcgacgtg gccgatcctg cacgaaggaa ataagcgcct
     2701 tccttattaa aggaagagaa gtccagacga cgggtgtcgg cgaaggggcc ataagcgccg
     2761 aatgaagcgc tggcatggcc ttcaaaaacg gatttgccgg tggtgacgct gaccacttga
     2821 aattcgtcag acataatgcg cgaatgactg gccagtacgg ctaccttgac tcccgccggc
     2881 ggatagccca tttggttgat gcggatccag gaagaggcag gctggccata ggtttgagca
     2941 agcgacgccc aaaagaaaaa agagaggatg agtttgatca cgactaaaga taaataaaac
     3001 ggttggaatc gtttaaattg cagtgtgtct aagatcctcc tcctgttttt tttgaccggg
     3061 atctcctctg tctcgaaggc gcagccttac gcgctactga tcaggggcgg gtatgtcatc
     3121 gaccccaaaa cggcggttcg cgacaccctc gacatcgcgg tacagaaggg gtgcatcgtc
     3181 aggatggcca aaaggattga tccgcgacaa gctgcaagga tcgtcgatgc ccgcggcgct
     3241 ttc
//
LOCUS       DASYSV010000238         5263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2218761, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000238 DASYSV010000000
VERSION     DASYSV010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218761"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2094)
                     /locus_tag="VGQ51_07335"
     CDS             complement(<1..2094)
                     /locus_tag="VGQ51_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEV9036413.1"
                     /translation="MKNSTRIFNWLLIGFFVFATGLSLQAQSVLDPTDAVVTYNPSSP
                     PPIPAYGSIAKWVRTVRMSWSTTNWKCYIYNGQQFRLRFPKSYQPGVNDGKQYPMLIF
                     MHGEGEQGTVYDNEYSLYHGGSLFESAVANGKYDGYVLVMQTGGGWGPNQFSAFKYII
                     DYMITNNKLDPFRVTLNGLSGGGQGTWQMYQTFPTYPAGLLPMSSDAIAYASADSAQK
                     WKFTSIWNIHGGLDGSPAPYTAQQVLLAMQAAGANYIDLNMTTQGHDTWDSTWSMPAF
                     WPWNLAQYSSNPWCLFGRTQFCPGDHISLTVGLTPGFQAYQWRKNGVLLSGTGNSINV
                     TDTGHYDARVERSGIWSDWSHTPVVISYSAPTVTPDITTSGLMSKVIPAPDGNSGVTL
                     FEPTGYATYVWQQVGNNNSIGSSNTIYANTPGQYTCQVTQQFGCSSSPSQAFTVINAN
                     GPNKPDPASGLVVSTISPTSLLLNWSQNPAPAFNETGFEIYQATHTGGPYKLIAITGQ
                     DASLDTVRGLSAGVKYYYIIRAVNNTAASANSNEASGTTTADSQAPTAPGNLIITGST
                     RSSISLDWTPSTDNVGVTGYYVYINGAKSYVVAPTQTSFTVGNLNYTQSYNFYITAFD
                     SAGNVSPASGQISGEPLFNGLNYQYYTFATSPSKLPNYNTLTQASAGTLPNVALTPAT
                     QTTNYGFLYQGTLHIT"
     gene            complement(2371..3117)
                     /locus_tag="VGQ51_07340"
     CDS             complement(2371..3117)
                     /locus_tag="VGQ51_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV9036414.1"
                     /translation="MEFERFADVKRLKFISDVLQAKLPTGAEVLDVGCGNGVISRSLG
                     EKGFAVRGIDVSDKAIARARELNKYPNVRFEVVSAEQLVADGHRYHAVICSEVLEHLN
                     DPGKLLSVLHQLLDENGVLIVTVPNGAGPRELFVTRPIIAMQRKNNWMWKAVQRTKRL
                     FGYKGTTVQSSADDLTHIQFFTKASLEKLASQHRFSIVRFGKTNFIEDVFPFSFFTKK
                     IKTLQKMDCAFAEILPYQWNGGFVTVWEKA"
     gene            complement(3107..4270)
                     /locus_tag="VGQ51_07345"
     CDS             complement(3107..4270)
                     /locus_tag="VGQ51_07345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9036415.1"
                     /translation="MTRKLRVLECIRQGRIGGGESHLLSLTSHLDRSRFEPVVLSFTD
                     GPMMEALRQMGVETRLIYTEKPFDIRIWGKVKRLLSDERIDVIHAHGTRAASNVLWAA
                     RSLGIPVIYTIHGWSFHRDQHPLVRGFRVWGEKWITKRAAVNISVSASNQQTGKEVMR
                     GFSSIVVNNGIDAKKFDPGGHYKDLRAELGIGGDRVLILFIARFTSHKQPLTLVRAFG
                     QASREIGAIRQTTGGAQIPEIHLLMVGDGDEKEEGVRLAKELGLEGRVSFQAFRQDVP
                     DVLAAADIFVLPSLWEGLPIGLLEAMAMRKAVIGTRVDGTREVLQDGVNGLMVDPGDV
                     NALSGAIVRLAGDKGLRESLRERAAETVRDRFNAAVMTKEIENIYTKIIPHGI"
     gene            complement(4273..>5263)
                     /locus_tag="VGQ51_07350"
     CDS             complement(4273..>5263)
                     /locus_tag="VGQ51_07350"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV9036416.1"
                     /translation="GGAGFIGSHVARHCLEMGFEVVVMDDLSGGFEDHIPEGAHFVKG
                     SVTDHELITDLFSEYRFTYVYHLAAYAAEGLSHFIRRYNYETNLVASINLINESVKHK
                     VKCFVFTSSIAVYGKGQLPMTEEMTPEPEDPYGVSKYAVELDLRAAHEMFGLNYVVFR
                     PHNVYGENQNIGDKYRNVIGIFMNQIMQGKKLTIFGDGEQTRAFSYIDDVAIPITKCV
                     NIPEAYNQVFNVGADEPYTVNELAKVVCKEFGVAPDIHHLNARNEVMHAYSDHGKARR
                     VFGAPTGISLQDGIGRMAAWAREVGARQGQEFSNIEITEKLPDGWAIVPKAVK"
BASE COUNT         1234 a         1596 c         1224 g         1209 t
ORIGIN      
        1 cgtaatatgc agcgtaccct gataaaggaa tccgtaattg gtcgtttggg tggcaggcgt
       61 cagtgcaaca ttcggtaatg tccctgcgga cgcttgcgtc agcgtattat agttcggcag
      121 tttgctcgga gacgtagcga aagtataata ctggtaattg agcccattga acaacggctc
      181 accgctgatc tgtccgctgg cgggagaaac attgcccgcg gaatcgaacg ccgtaatata
      241 gaaattgtat gattgcgtat agttcagatt accgaccgta aagctcgtct gggtaggcgc
      301 gacgacataa gatttagcgc cgttgatata aacataatat ccggtcacac ctacattatc
      361 ggtagaaggc gtccagtcga gagagatcga cgaccgcgtg cttcccgtaa tgatgagatt
      421 acccggtgcc gtgggcgcct ggctgtcggc cgtggtcgtg ccgctggcct cattggaatt
      481 ggccgacgca gccgtgttgt tcaccgcacg gatgatataa taatatttca cacccgcgga
      541 caatcccctg accgtatcca ggctcgcatc ctgacccgtt atcgctatca gcttgtaagg
      601 tccgccggta tgcgtcgcct ggtagatctc gaatccggtc tcattgaagg caggcgcggg
      661 attctggctc cagttgagga gcagcgaagt cggcgaaatt gtgctgacca ccagacccga
      721 agccggatcg ggcttattgg gtccgttcgc attgatcacg gtaaaagcct gcgagggact
      781 gctcgaacaa ccgaactgct gcgtcacctg acaggtatac tgcccgggcg tattcgcata
      841 aatagtattg gaggaaccga tcgaattgtt gttacccacc tgctgccaga cataagtggc
      901 atatcccgtg ggttcaaaca gggtaacacc tgaatttcca tccggagccg ggattacttt
      961 gctcatcagc ccgctcgtgg tgatatccgg agtcaccgtc ggagcgctat atgaaatgac
     1021 cacgggcgta tgcgaccagt ccgaccagat accgctccgt tctacacgcg cgtcataatg
     1081 tcccgtatcc gtcacattga tcgaattgcc cgtgccggaa aggagcaccc cgttcttccg
     1141 ccattgatag gcctggaacc cgggcgtcaa tcccaccgta agactgatat gatcccccgg
     1201 acagaactgc gtccttccga acagacacca gggattcgag ctgtattggg ccagattcca
     1261 cggccagaag gccggcatcg accacgtact gtcccatgta tcatgcccct gcgtggtcat
     1321 gttcaggtcg atatagttcg cccccgcagc ctgcatggcc aataacacct gctgtgccgt
     1381 ataaggcgcg ggcgacccat ccagccctcc atggatattc cagatggagg tgaacttcca
     1441 tttctgtgcg gaatcggcgc tggcgtatgc aatggcatcc gacgacatcg gcaacaaacc
     1501 cgccgggtaa gtggggaagg tctggtacat ctgccatgtg ccctgaccgc cacccgaaag
     1561 accattgagc gtcacacgga atggatccag cttattgttg gtgatcatgt aatcgatgat
     1621 atacttgaag gcgctgaact ggttggggcc ccagccaccg ccggtctgca tcaccaggac
     1681 atatccgtcg tatttaccat tcgccacggc cgattcgaat aaactaccac catgatacaa
     1741 cgaatattca ttgtcataca cggttccctg ctcgccttct ccatgcatga agatcagcat
     1801 gggatactgc ttcccatcat tgacgccggg ctgataactc ttggggaatc gtaaccggaa
     1861 ctgctgtccg ttatagatgt aacacttcca gttggtagtg ctccaactca tccttaccgt
     1921 tcttacccat ttggcgatcg agccataggc gggaataggg gggggactgc tcggattgta
     1981 ggttacgacc gcgtcggtgg ggtccaggac gctctgagcc tgtagggaca ggcctgtcgc
     2041 gaaaacgaaa aagccgatca acagccaatt gaaaatccgg gtagaatttt tcattttttt
     2101 tgcttaattg ttaacgtatg cctgcgatgg cagacacaag tccacatagg cactcgattt
     2161 aagcgcaatg tttttgaaag gataaaattg gaaggaaagg tgacgttaga gaagaatcgg
     2221 taaaggggtt gatcgtagga aaattagaag ggaaaaatta atggcgcaaa atacatcctt
     2281 ttctctattc ttgcaagcga acctttaaat acttcaaaaa tattaaccgg tacataacac
     2341 gtattagccg gtctacctca tccatgtggg tcatgccttc tcccacaccg tcacgaaccc
     2401 gccgttccac tgataaggca atatctccgc aaacgcgcaa tccatcttct gcaacgtctt
     2461 gatcttcttg gtaaaaaagg agaaagggaa cacatcctct ataaaattgg tcttcccgaa
     2521 tcgcacgata ctgaaccggt gctggctcgc caatttctcc aaagaagcct tggtaaaaaa
     2581 ctggatatgc gtcagatcgt cggcgctgct ctgcaccgtc gtacccttat agccgaaaag
     2641 acgcttcgtc cgctgcacgg ccttccacat ccagttgttc ttacgctgca tcgcaataat
     2701 gggtcttgtg acaaacagct ccctgggccc tgcgccattc ggaacggtca cgatcagtac
     2761 cccattctca tcgagcagct gatggagtac gctcagcaat tttcccggat cattgagatg
     2821 ctccagcact tcgctgcaga tcacagcatg ataacgatgc ccgtcggcca ccagttgctc
     2881 cgcgctgacc acctcgaacc ttacgttggg gtatttgttc agctcccgcg ccctggcgat
     2941 cgccttatcg ctcacgtcga tacccctcac ggcaaaacct ttctccccga gacttctcga
     3001 gatcacaccg ttgccgcagc cgacatccag cacttcggcg ccggtaggca gcttcgcctg
     3061 caacacatca ctgataaatt tcagccgttt gacatcggcg aaccgttcaa attccatgag
     3121 gaatgatttt tgtataaata ttctcgatct ctttagtcat gacagcagca ttgaaccgat
     3181 ccctgaccgt ctcagccgct cgctcccgaa gcgattcccg cagccccttg tcacccgcaa
     3241 gccgcacgat cgctcccgac agcgcattca catccccggg gtccaccatc aacccgttca
     3301 cgccatcctg caggacctcg cgggttccat ccacccgcgt tccgatcacc gccttccgca
     3361 tcgccatcgc ctccagcaac cctatcggca acccctccca caaggaaggc agcacaaaaa
     3421 tatccgccgc cgcaagcaca tccggcacat cctgccggaa tgcctggaaa ctcacccgac
     3481 cctccaaacc cagctccttc gccaaccgca ccccctcctc cttctcgtcc ccatccccga
     3541 ccatcagcaa atggatctcc ggtatttgcg cgcccccggt cgtttgccgg atcgccccta
     3601 tttcccgcga cgcctgacca aacgcacgaa caagcgtcaa cggttgctta tgtgatgtaa
     3661 accttgcaat aaataatatc agaacccggt cgccgccaat tcccaactcc gcccgcagat
     3721 ctttatagtg cccacccgga tcgaattttt tcgcgtctat cccattattc accactatcg
     3781 aagaaaatcc gcgcatcacc tccttccccg tctgctgatt gctcgcggaa acggaaatat
     3841 tcacagccgc ccgcttcgtt atccatttct cgccccatac cctgaacccc cgcaccagtg
     3901 gatgctgatc ccggtgaaat gaccacccgt ggatcgtgta gatcaccggt atccccagag
     3961 accgcgccgc ccataacaca ttcgatgcgg ccctggtccc atgggcatgt ataacatcga
     4021 tccgctcatc cgataacagc ctcttcacct tgccccatat ccggatatcg aacggctttt
     4081 cagtatagat caacctcgtt tcgactccca tctgccgcaa ggcttccatc atcggaccat
     4141 ccgtaaaaga aagaaccacc ggttcgaatc tcgacctgtc cagatgactg gtcaggctca
     4201 gcaaatgcga ctctccgcca cctatcctgc cctgccggat acattcaagc accctgagtt
     4261 tccgagtcat ggctatttta cggctttggg tacaatggcc cagccatccg gtaatttctc
     4321 cgtgatctcg atattgctga actcctggcc ctgacgagcc ccgacttccc tcgcccacgc
     4381 cgccatccgg ccaatcccgt cctgcagact gattcccgtc ggggcgccaa aaaccctccg
     4441 cgccttgccg tgatccgaat aggcatgcat cacctcatta cgggcattca gatgatggat
     4501 gtccggtgcc accccgaatt ccttgcaaac caccttagcc agttcattca ccgtataagg
     4561 ctcatcggcg ccgacattga acacctggtt atacgcttcc gggatgttca cgcatttggt
     4621 aatggggatc gcgacatcgt cgatatagct gaacgccctc gtctgctcac cgtcaccgaa
     4681 gatcgtcagc ttctttccct gcatgatctg gttcatgaat atgccgatga cgttgcgata
     4741 cttatccccg atattctgat tctccccata tacgttatgg ggccggaata cgacgtaatt
     4801 aagtccgaac atctcatgcg cagcccgaag atccagctcg actgcatact tgctgacgcc
     4861 atacgggtct tccggctccg gcgtcatttc ctcggtcatc ggcaactgtc ccttgccgta
     4921 tacggcgatg gaggacgtga aaacaaaaca cttcactttg tgctttaccg attcattgat
     4981 aagattgatg ctggcgacga gattcgtctc ataattatac cggcggatga aatgagaaag
     5041 accctccgcc gcataagccg ccagatgata gacataagtg aaccggtact cggaaaagag
     5101 gtccgttatc agttcatgat ccgtaacact acccttaacg aaatgggcgc cctccggaat
     5161 atgatcctcg aaaccgccgc tcagatcatc catgacgacg acttcgaaac ccatttccag
     5221 acaatgcctg gctacatgcg atccgataaa ccccgctccc ccc
//
LOCUS       DASYSV010000239         9634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_168931, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000239 DASYSV010000000
VERSION     DASYSV010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168931"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VGQ51_07355"
     CDS             complement(<1..1423)
                     /locus_tag="VGQ51_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEV9036417.1"
                     /translation="MSNQIVSIFTSFYNDLVYSGLKDEHSPTKRKKTIRFNQFILLIL
                     VAHFVCVISYFYFGLYISALINLSTAYIFIVAYHLNLRKRYLLARVISVLNLNFYLAV
                     MNYMEGLRAGEFLFFFPYFLIMTFIISFTRNLRELLFAYGTTVACILFCFYWCPQENH
                     YQVHIAGLFDKVYDSNLVLSLLLTIFFSFAIVRINRNNEEAILKEKRFVDTIYNTSLD
                     GIFILDAEHLVITDCNNRAVELLEIEDKQQIVGAPMDSLFTKEHIGLFRTVGLKEGQV
                     GSWQGELALTSKKDNTVFAFGNVVSFFDNDRHFWKISILDISEIKMTQFELMKAKQKA
                     ETASKAKSRFLSNMSHELRTPLNGIIGASNLLMQEQYLPEQLSHLEILKYSSEHMMVL
                     INEILDFNKIEAGRFELENVPVNMRAFLRKLETQFSVQAHAKGLQFLLDIDQQLETEF
                     LTDETRLNQVLGNLLSNAIKFTHK"
     gene            1517..1912
                     /locus_tag="VGQ51_07360"
     CDS             1517..1912
                     /locus_tag="VGQ51_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEV9036418.1"
                     /translation="MEQPLSDRTFRFSDDINSQYIIELYAGDYVMIEETFTDVLNEYE
                     GFVQRIITSYHEKDRQALKSAVHKVKPLFGFVGLTPLQSQCLQFENSCLEIEETKLDS
                     SFNALMEKLTEARSIIEKEKARLMEFNRQ"
     gene            complement(1929..3077)
                     /locus_tag="VGQ51_07365"
     CDS             complement(1929..3077)
                     /locus_tag="VGQ51_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV9036419.1"
                     /translation="MDYKRMPIEIESPEQLGYDKIRNNLSESSYTDTLLRDLPGRDDL
                     RDLALCYGSHQGHEGLRGLIAKEHATLKADHMPLEADHVLLTMGAAGALFILATSLLE
                     KGDELIVVHPNYATNIETPRAIGAALRTIGLRFEEGFAPDISRIEEAITPKTRYISVT
                     HPHNPTGVCLTNDQLQRLADIAENKGIYVLVDETYRDMVFGEQLPPAATWSPRIISIS
                     SLSKTYGLPGLRVGWIICRDAGLMETFLAAKEQIHICGPVLEEELAYRYLQHRHAHLT
                     RIREDIRGKFRIMKDWMLRGEEWEWIEPQGGCVCFPRLRRPHDIDVEVFYRVLLDRYG
                     TYVGPGHWFDMPDHYMRIGFGWPSAGSLEEGLSAMSKAVKDAWKNPGR"
     gene            3178..4269
                     /locus_tag="VGQ51_07370"
     CDS             3178..4269
                     /locus_tag="VGQ51_07370"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV9036420.1"
                     /translation="MRLPYLKQLDGVRAIAALMVMFLHFFNGLGGNQSLLGILGHYAV
                     IGQTGVSLFFVLSGFLITRILLVTKEGPHYFSDFYIRRVLRIFPLYYLFLIIYYFFVP
                     LLVHSSSVPFSGQVYYWVYLQNFAETFKWHAVGPPHFWSLAVEEHFYLLWPCLVFFLS
                     KKGIKIAVVVILFLAVGCRLELLREGREVYYFTLTRMDDLAIGALLAVWETEGRLKGS
                     AKKFAIGLVLTVIPTILLWKVVTGKSIGSMQVIKFNLVALCGFCLVGFAIAARETSFF
                     YKLMSSRFFRFTGRISYGLYVYHPLCYPLIEYYFPTISVPARFLVSFGATYLIAGLSY
                     YLFEAKFIALKKYFAYGRDRTTHFSTFGA"
     gene            4266..4706
                     /locus_tag="VGQ51_07375"
     CDS             4266..4706
                     /locus_tag="VGQ51_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEV9036421.1"
                     /translation="MKRRLALTLIIGLLAFGKGYAQQSAAEAEKDIVASMNDATTKWN
                     TGDLNGYMALYDPTATMMTSGGRVGIDSIRRIYVVHYFQDGHPKQELAYDTYQITMLG
                     KDYALLTGRFILKANGKLPERHGSFSLTMVHRKNGWRILHDHSS"
     gene            complement(4756..5898)
                     /locus_tag="VGQ51_07380"
     CDS             complement(4756..5898)
                     /locus_tag="VGQ51_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEV9036422.1"
                     /translation="MNKQREFRMGSENEDATPETQTPGEDTPAQPAPYLKGRGAQFNP
                     KNRFLKNERVKEHIEAIDEWVEPNIATQYLEDHSKSIVNKVDSPDVGMWYSMNPYQGC
                     EHGCTYCYARNAHEYWGYSAGLDFERKIIVKKNAPELFRKFLMDPKWECLPISISGNT
                     DCYQPAEKKFGITRRLLEICLEFNQPVGMITKNAGILRDKDLLTQLAKKKLVSILVSI
                     TSLDEDLRQLMEPRTTTARQRLRTIKELSDAGVRMGVMLGPMIPGLNEHEMQRIMKAA
                     ADNGASFSAYTFIRLNGAIKLIFRDWLYKSFPDRADKVWHLIEASHDGKVNDSRWGVR
                     MRGEGNIAGLVAQQYARYTKKYGLNEERWELDCSQFRRPGGQMKLF"
     gene            complement(5903..6583)
                     /locus_tag="VGQ51_07385"
     CDS             complement(5903..6583)
                     /locus_tag="VGQ51_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036423.1"
                     /translation="MQTFKPVSTILNENYKSQLDAAEYQLILSPHEELNNKIRQIRTV
                     FQETYQASSVPGGKANLALARFTQLTMMEERIVQRLRTIAMGFCPFKVELKDFGALPT
                     HSIFINMTSKLPIRALVKEIRDTQRLMKLDKEHKPHFIDEPNILIARKLQPWQFEKGW
                     LEYAGKSFTGRFIADAMLLLRRRANAVLQEGRGRDTRPGNWQIVGRFEFRNLPVSTRQ
                     GELFTSEI"
     gene            complement(6596..7372)
                     /locus_tag="VGQ51_07390"
     CDS             complement(6596..7372)
                     /locus_tag="VGQ51_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEV9036424.1"
                     /translation="METGLVTQSNILSSPLRGQTSAGIGVAGGVVAERGDRRAEYLLV
                     IYPYGELRDRLLEEQQQFSTDYEVTVRNRPHITMAVFQAAEGMEDTLIRWIQRICQRY
                     QRFELTLNNYSGIPPHTTKPAEPACPPGRSLAVCLDSLNPTQRIPGTIYLRVQDPQPF
                     RRLMQQLRAIDHYVRSSGCPPINLIDRPYLSIAGGLTEQVYNRAMPDYSRKIFHESFP
                     VDELVLLKRNHQFDPCRTVNIFRLQPADTSGVSAGTLPHL"
     gene            7463..7939
                     /gene="umuD"
                     /locus_tag="VGQ51_07395"
     CDS             7463..7939
                     /gene="umuD"
                     /locus_tag="VGQ51_07395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEV9036425.1"
                     /translation="MELEQFFGIQYKGSKRFTQWDVPAANSNDCPSDKTLPESFATGF
                     GATADDYMERGIDLNEQLIRNKPATFFMRVSGDAMTGAGIFNGDVVIVDRSVKAASGK
                     VVIASLNGEMLIRRLEKNLNKLRLVQETPKLSPIDVDVSGAEFSIWGVVTYVIHAV"
     gene            7936..8157
                     /locus_tag="VGQ51_07400"
     CDS             7936..8157
                     /locus_tag="VGQ51_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036426.1"
                     /translation="MIAIIDCNSFYCSCERLFRPDLRDKPVAVLSNNDGCIISRSDEA
                     RHLGCHPSSSQRPHPPADQSGGRIGGSAV"
     gene            8126..8293
                     /locus_tag="VGQ51_07405"
     CDS             8126..8293
                     /locus_tag="VGQ51_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HEV9036427.1"
                     /translation="MAVGLVDQLFENGKTYKKAGVMLSGLVPDDSIQGMRQELRSKRH
                     TTRWEELKEVI"
     gene            8415..8969
                     /locus_tag="VGQ51_07410"
     CDS             8415..8969
                     /locus_tag="VGQ51_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9036428.1"
                     /translation="MSASGANNMASVIARFGKRLLGFIRRRVGNEADAEDILQDVWYQ
                     LTMAVDTTPIEQMSGWLFAVARSKIIDRYRKKHPESLDRLLQLSEEEGGSELADLLLD
                     NSSNPERAHLKALFWKELQDALDELPEEQRAVFVWNELGELSFKEIAELTGENVNTLI
                     SRKRYAVLHLRERLLTLYHDIIEH"
     gene            8982..9383
                     /locus_tag="VGQ51_07415"
     CDS             8982..9383
                     /locus_tag="VGQ51_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036429.1"
                     /translation="MRRFWIRRAPFFILMASAGVIVFVIIVMLLWNELMPAIFHLPVI
                     TFWQALGLLVLSKIFFSSLRGPRPYWRNKARQKWMDMTPEERDRFREEWGRRCGGPGA
                     GRAPWSEGRQRSGGEERPAAAQPDENQRSPL"
BASE COUNT         2153 a         2368 c         2697 g         2416 t
ORIGIN      
        1 ctttgtgggt gaatttgatg gcgttggaca agagattgcc caacacctgg ttaagccgcg
       61 tttcgtcggt aaggaattcc gtttccagct gctgatcgat atccagcagg aactggaggc
      121 ctttggcgtg cgcctgtacg gaaaactggg tctccagctt ccggaggaaa gcccgcatat
      181 tgaccggtac attctccagc tcgaaacggc cggcttcgat cttgttgaag tccaaaatct
      241 cgttgatcag gaccatcatg tgttcggaag aatatttcag tatttccaga tgagacagtt
      301 gctctggaag atattgctcc tgcattagca gattgctggc gccgatgata ccgttgagcg
      361 gggtccgcag ttcatggctc atattggaca ggaaccggga tttggctttc gaggccgtct
      421 cggctttttg tttggccttc atcagctcga actgcgtcat tttgatctcg gagatatcga
      481 ggatgctgat cttccagaaa tgccggtcat tgtcaaaaaa agaaacgacg ttgccgaacg
      541 caaacaccgt attgtctttt ttggaagtga gtgcaagctc gccctgccag ctaccgacct
      601 ggccctcctt tagcccgacg gtcctgaaca gcccgatatg ttctttggta aagagactgt
      661 ccatgggcgc gccgacgatc tgttgtttgt cttcgatctc gagcagctcg accgcccggt
      721 tgttgcaatc ggtgatcacg aggtgttccg catctaggat gaaaatgccg tccagggagg
      781 tattatagat cgtgtcgacg aaccgttttt ccttgaggat ggcctcttcg ttgttccggt
      841 tgatccgtac gatggcgaag gaaaagaaga tggtcagcag gagcgaaagc acgagattgc
      901 tgtcatacac cttgtcgaag agtcccgcga tatgcacctg gtaatggttt tcctgcgggc
      961 accagtagaa gcagaagagg atgcaggcga ccgtcgtccc ataggcgaag agcaactcgc
     1021 ggagattgcg ggtgaagctg atgatgaagg tcatgatcag gaagtagggg aagaagaaca
     1081 gaaactctcc cgcccggagt ccttccatgt agttcatcac cgcgaggtaa aaattgaggt
     1141 tcagcaccga tatcacgcgt gccagcagat agcgtttgcg gagattgagg tgataggcca
     1201 cgatgaagat gtaggccgtg ctgagattga tgagcgcgct gatatagaga ccgaaataga
     1261 aatacgagat gacgcagacg aagtgagcga cgaggatcag caggatgaac tgattgaatc
     1321 gtatcgtctt tttccgtttg gtgggggaat gctcgtcctt tagcccggag tagactaaat
     1381 cgttgtaaaa ggacgtaaaa atagatacga tctgattgct cacaaggtgc gaatacggtg
     1441 gtttggaggt ttaaaggact tcaacgtcta aagacaatat tttagtatgg gtgtcgttaa
     1501 tttacgaagc ctttctatgg agcagccttt atccgacagg acattccgat tcagcgacga
     1561 catcaatagt caatatataa tcgaattata tgcaggggat tatgtgatga tcgaggagac
     1621 gttcaccgat gtgttgaacg aatacgaagg ttttgtccag cggataatca ccagttacca
     1681 tgagaaagac cggcaggcgt tgaaaagcgc cgtccataag gtaaaacctt tattcggctt
     1741 cgtggggttg accccgctgc aatcccaatg tctgcaattc gagaattcct gtctggagat
     1801 cgaggaaacg aagcttgatt cttcttttaa cgcactaatg gagaaattga cggaagccag
     1861 gagcatcatt gaaaaagaga aagcgcggtt gatggagttc aaccggcaat gagccgcgcc
     1921 cgcttcgatt agcgccccgg gtttttccac gcatccttta cagccttcga catcgcgctc
     1981 aatccttctt ccagacttcc ggctgagggc caaccaaatc cgatccgcat atagtgatcg
     2041 ggcatatcga accaatgacc cggtcctacg tacgtgccgt agcgatccag caggacccgg
     2101 taaaaaactt cgacatcgat gtcgtgtggc cgtcgcaatc ttgggaagca aacgcatcct
     2161 ccctgaggct cgatccattc ccattcttcc ccgcggagca tccagtcttt cattatccgg
     2221 aacttccccc tgatatcttc gcggatgcgg gtcaaatgag catgccggtg ttgtaaatac
     2281 cggtaggcca attcttcctc caataccggt ccgcaaatat gtatctgttc ctttgccgcc
     2341 agaaaggtct ccatcagacc ggcgtcgcgg cagatgatcc aaccgacgcg caggccgggg
     2401 aggccatacg tttttgagag cgaggagatg ctgatgatgc ggggagacca ggtagccgcc
     2461 ggaggcagct gttcgccgaa gaccatatcc cggtaggtct catctacgag tacatagatg
     2521 cctttgtttt cggcgatgtc cgcgagccgc tgaagttgat cgttcgtcag acaaaccccg
     2581 gtggggttat gtggatgtgt caccgaaata taacgtgtct tgggggtgat cgcttcctct
     2641 atccgggaga tatcgggggc aaagccttct tcgaacctca gtccgatggt gcgcagggct
     2701 gcgccgatag cccggggtgt ctcgatattg gtggcatagt tgggatggac gactatcagc
     2761 tcgtctcctt tttcaagaag gctggtagca aggatgaaca gggcgcctgc ggcgcccatg
     2821 gtcagcagca catggtccgc ttcgagcggc atgtggtctg ctttgagcgt cgcgtgctct
     2881 ttcgcgatga ggccgcgaag cccttcatgt ccctggtgcg aaccatagca aagcgcgaga
     2941 tcgcggagat catctctacc gggaagatcc cgtaagaggg tgtcggtata ggagctttcg
     3001 gatagattgt tgcggatctt atcatatccg agctgttccg gcgattcgat ctcgataggc
     3061 attcttttat aatccatgag cgatggtatt agaatatgag gacaaaaaat taaaaaattt
     3121 ccaagggtat gcgaaatctt tttgccgact gatcgttttt tcgatacttt gagcgggatg
     3181 agacttccat atctgaaaca gctggacggc gtaagggcca tcgcggccct gatggtgatg
     3241 ttcctgcatt tcttcaacgg tctcggcggc aatcagtcat tgttgggtat cctgggacat
     3301 tatgcggtga tcggccagac gggggtatcg ctgttcttcg tcctgtcggg tttcctgatc
     3361 acgcgtatct tactggtgac aaaggagggg cctcattatt tttcggattt ttatatccgg
     3421 agggtcctgc ggatcttccc gctttattat ctttttctga tcatttacta tttcttcgtt
     3481 ccgttattgg tgcattcatc gtcggtccct ttttccgggc aggtatacta ttgggtgtac
     3541 ctgcagaatt ttgcagagac cttcaaatgg cacgctgtcg ggccacctca tttctggtct
     3601 ttggccgtcg aggagcactt ttatctgctt tggccttgtc tcgtgttttt tctcagcaag
     3661 aaagggatca agattgctgt cgtggtgatc ctttttttgg cggtgggatg tcgtctggaa
     3721 ctgctgcgtg agggacggga ggtctattat tttacgctga cccggatgga cgatctggcg
     3781 atcggcgctt tattggctgt ttgggaaaca gaaggccggc tgaagggaag cgccaaaaaa
     3841 ttcgccatcg ggctagtgct tacggtcatt cctacgatcc ttttatggaa agtggtcaca
     3901 gggaaatcga tcggcagcat gcaggtgatc aagttcaatc ttgtcgcctt atgcgggttt
     3961 tgcctcgtag ggttcgccat cgccgcgagg gagacgagtt tcttttataa attgatgtcg
     4021 agccggttct ttcggtttac cggaaggatc agctatgggc tgtatgttta tcatcctttg
     4081 tgctatccgc tgatcgaata ttatttcccc accatttccg tgccggcgcg gttcctggtc
     4141 agttttggag cgacctacct gattgcaggt ctcagttact atctttttga agcgaagttc
     4201 attgccttga agaaatactt cgcgtatggg cgggatcgga ccacccattt tagtacattc
     4261 ggtgcatgaa aagacgactt gctctcacac tcatcatagg gctgctggct ttcgggaagg
     4321 gctatgccca gcaaagcgca gctgaagccg aaaaagacat cgtcgcctcc atgaacgatg
     4381 cgacaaccaa atggaatacc ggcgacctca acggttatat ggccctatat gatccgacgg
     4441 ccacgatgat gacctccgga ggccgcgtag ggatcgacag catccgccga atttatgtcg
     4501 tccactattt ccaggatggc catcccaagc aggagctggc ttacgacacc tatcaaataa
     4561 ccatgttggg caaggattat gccttgctga cgggaaggtt catcctgaag gcaaacggca
     4621 agctgcccga gcgtcatggg tcgttttcgc tgaccatggt gcatcggaag aatggatgga
     4681 ggatactcca tgaccattcg agctagttgg cgccgccgac gtttccgcag cccgcggctt
     4741 gtttcgccga caatctcaaa acaatttcat ctgacctccc ggacgccgga actgcgagca
     4801 gtccagctcc catcgctctt cattcagccc gtattttttt gtataccgcg catattgctg
     4861 tgccaccagc ccggcaatat tcccttctcc gcgcatacgc actccccacc ggctatcgtt
     4921 gaccttgcca tcatggctcg cctcgataag atgccagacc ttatcggccc tgtcgggaaa
     4981 actcttatag agccagtcgc ggaagatcag ctttatggca ccgttgagac ggataaaagt
     5041 ataggctgag aagctggccc cgttgtccgc cgcggctttc atgatgcgtt gcatctcatg
     5101 ctcgttgaga cccgggatca tgggccccag catcaccccc atccgtacgc cggcgtcgct
     5161 aagctctttg atggtccgaa gccgttggcg cgcggtggtc gtcctcggct ccatgagctg
     5221 tcggaggtct tcatccaaag aggtgatcga taccaatatc gataccaatt ttttcttagc
     5281 cagttgggtg agcaggtcct tatcgcggag gatgccggca ttcttggtga tcatgccgac
     5341 gggctggttg aattccagac agatctccag gaggcggcgg gtgatcccga attttttttc
     5401 cgcaggctgg tagcaatcgg tattcccgct gatggagatg ggcaggcatt cccatttggg
     5461 gtccatcagg aatttgcgga acagctcggg tgcattcttc ttgacgatga tcttgcgttc
     5521 aaaatcgagc cctgcgctat agccccagta ttcatgcgca ttccgcgcat agcaataggt
     5581 gcagccatgc tcgcagccct ggtaggggtt catgctgtac cacatgccga catcggggct
     5641 atccacctta ttgacgatgc ttttagagtg gtcctcgagg tattgggtgg cgatattggg
     5701 ttcgacccat tcgtcgatgg cttctatgtg ttcctttacc cgttcgtttt tgagaaaccg
     5761 gttcttagga ttgaattggg ccccgcggcc tttcagatat ggagcgggtt gcgcgggggt
     5821 atcctcgccc ggcgtctggg tttccggcgt tgcatcttca ttttcacttc ccatccggaa
     5881 ttcccgttgt ttattcatgt gcttatattt cagaagtaaa taattcgccc tgccgggtgc
     5941 ttaccggcag attgcggaat tcgaatcgcc cgacgatctg ccagttcccg ggtcgtgtat
     6001 cgcgaccgcg gccctcctgc agaacagcat tcgcccgtcg acgcaaaaga agcattgcat
     6061 ccgcgatgaa tctgccggta aagctctttc cggcgtactc gagccatcct ttctcaaatt
     6121 gccagggttg cagcttacgg gcaatgagga tattgggctc atcgatgaaa tgtggcttgt
     6181 gctccttatc cagcttcatg aggcgctggg tatccctgat ctcctttaca agcgccctga
     6241 tcggcaattt gctggtcatg ttgataaaaa tgctgtgggt gggcagagcg ccgaaatcct
     6301 tcagctccac tttgaagggg caaaatccca tggcaatggt cctgagccgt tgtacgatac
     6361 gctcttccat catcgtcagc tgcgtgaacc gcgccagcgc cagattcgcc ttgccgcccg
     6421 gcacggagga agcctgatag gtctcctgga aaacggttct gatctgtctg atcttattgt
     6481 tcagctcttc atgtgggctc aggatgagct ggtattcggc agcgtcgagc tggcttttat
     6541 agttttcgtt gagaatggta gaaacaggtt tgaaggtttg catagacttg aagttttaaa
     6601 gatgaggcaa ggtgccggcg gatacgccgg atgtgtcggc cggttgcaac cggaagatat
     6661 tgaccgtccg gcaggggtcg aattgatggt tccttttcag aagcaccagc tcgtccacgg
     6721 gaaagctttc atgaaagatc tttcgggaat aatcgggcat ggcccggttg tagacctgtt
     6781 ccgtcagtcc ccccgcaatg ctgagatagg gccggtcgat caggttgatg ggcggacatc
     6841 ccgaactgcg gacataatgg tcgatggccc gtaactgttg catcagccgt ctgaaaggtt
     6901 gcgggtcctg tacgcggaga tatatggtgc cggggattcg ctgtgtggga tttagcgaat
     6961 ccaggcaaac cgctaatgat cgtcctggcg gacaagccgg ctccgccggc ttagtggtgt
     7021 gcggagggat gccgctgtag ttgttgagcg tcagttcgaa acgctgatag cgttgacaga
     7081 tgcgctggat ccatcggatc agggtgtctt ccatgccttc ggcggcctgg aaaacggcca
     7141 tggtgatgtg aggacggttg cgcaccgtca cttcataatc ggtagaaaat tgctgctgtt
     7201 cttcaagcag acggtcgcga agctcgccgt aaggataaat gaccagcagg tattccgccc
     7261 tgcggtcgcc gcgttcggcg acgactcctc ccgcgacgcc tattccggcg ctggtttggc
     7321 cgcgtagcgg cgatgacaag atgttcgatt gtgttactaa cccggtttcc ataaaaagat
     7381 tttaaacgat caataattta gtataaattt actaaacaaa ttagtaagta caaattattt
     7441 tttctctcca accaattctt ttatggaact cgagcaattt ttcggcatcc agtacaaggg
     7501 gtcgaagcgt ttcacgcaat gggatgtccc ggctgcgaac tccaatgact gtccttcgga
     7561 caagaccctt ccggagtctt ttgctaccgg tttcggggcc accgcggacg attatatgga
     7621 aaggggtatc gatctgaacg agcagctgat ccgcaataaa ccggctacat ttttcatgcg
     7681 ggtaagcggc gatgccatga ccggcgccgg tattttcaac ggggatgtcg tcatcgtgga
     7741 ccgcagcgtc aaggcggcga gcgggaaagt agttatcgcg agcctcaacg gggaaatgct
     7801 gatccgtcgt ctcgaaaaga atttaaacaa gctgcggctc gtgcaggaaa ccccgaagct
     7861 gtcacctatc gatgtggacg tctccggggc cgagttctcg atctgggggg tggtcactta
     7921 tgtcattcac gccgtatgat cgctatcatc gattgtaaca gcttttactg ctcgtgcgag
     7981 cgactattcc ggcccgatct ccgggataaa cccgtcgctg tgttaagcaa taacgacggt
     8041 tgcatcatct cgcgcagcga cgaagcgcgt catctggggt gccacccttc ctcatcccag
     8101 cgccctcacc caccagctga tcaaagtggc ggtcggattg gtggatcagc tgtttgaaaa
     8161 tggcaagacc tataaaaaag cgggagtgat gttaagcggg ctggtgcctg acgactctat
     8221 ccaggggatg cggcaggagt tgaggtcgaa aagacatacg acgagatggg aggagttaaa
     8281 ggaggtgata taaaagttcc gtaaaaaatt taaaatattt cccgcactcg cccgtctacc
     8341 tttatgtggg ctttcggccg gatggccggc ggccggcttt tgaaatgggg caacctgcaa
     8401 tcatatcagc gagaatgtct gcatccgggg caaacaatat ggcctccgtc atcgcgcggt
     8461 tcggcaaacg gctgctgggt tttatccggc ggcgtgtcgg caacgaggcc gacgcggagg
     8521 acattttgca ggatgtttgg tatcagctga cgatggccgt ggatacgact ccgatcgaac
     8581 aaatgagcgg ctggctgttt gccgtggcgc gcagcaagat catcgaccgc tatcgaaaaa
     8641 agcatccgga gtcactggat cgtttgctcc agctgagtga agaagaaggc gggtcggagc
     8701 ttgcggatct gttgctggat aacagcagca acccggaaag agctcatctg aaagcgctgt
     8761 tctggaagga attgcaggat gctttggacg agctcccgga agaacaacgc gcagtctttg
     8821 tctggaatga gctgggcgag ttgtcgttca aggagattgc ggagctgacg ggagagaatg
     8881 tcaataccct tatctcccgg aagagatatg cggtgttgca tctgagggaa cggttgctga
     8941 cactttacca cgatattatc gaacactaaa aaataaattc tatgagacgt ttttggatac
     9001 gcagggcgcc ctttttcatt ttgatggcaa gtgcaggcgt catcgtcttc gtgatcatcg
     9061 tcatgctatt gtggaacgag ctgatgccgg cgatctttca tcttcctgtc attaccttct
     9121 ggcaggcgct ggggctcctc gtgctcagca aaatattctt cagcagtttg cgcgggccca
     9181 ggccatattg gaggaataag gcaagacaaa aatggatgga tatgacgccg gaagagcggg
     9241 atcggttcag ggaagaatgg ggccgtcgtt gcggaggacc gggagccgga cgggcgccct
     9301 ggtcggaggg gcgtcaacgg tccggggggg aagagcggcc ggccgcggca cagcccgatg
     9361 aaaatcagcg ctctccgctt taatcaatct ttaacagtcg gcgattaaat cccgaagctt
     9421 ttttcatatc caatagacaa ggatgcaaaa acgaaaacaa atgcatctgc taacttaaaa
     9481 aactacagat atgaaaaagc tagccatctt caccgtcatc gcagccgcga gcctcatcgc
     9541 tacaaaaagc tactcacagg tatatgtcaa tgctcacgtc ggatttcgcg ttcccggtgg
     9601 acgggtctat gtcggcacag caccggcgcc tccg
//
LOCUS       DASYSV010000240         5327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_815972, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000240 DASYSV010000000
VERSION     DASYSV010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815972"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..2168
                     /locus_tag="VGQ51_07420"
     CDS             138..2168
                     /locus_tag="VGQ51_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9036430.1"
                     /translation="MKYFRPMLLLLLQALSMLVAAQPNANDSLLDIIRQDRRDLSEAR
                     ALNALAFNYARTDLAKAKGCLYRSIELASAGHFGVQLSAAYTQMVFCQQDVGQVDSAG
                     VFLRLLKHLSDSDANLRSNYVQAAGLFYKRQQNFKAAMPFLLEALDRAKAAAAADPSV
                     VKRTAVAGAFLNAGNNYDELGDYRMALWNHLDALRLFEAVDNKKGISFAYQTIGGDFL
                     LLNQLQSASDYTKRSLAIKTELNDQRGIATSLKQLGSVFRSGKHLDSALDYYGRSIAI
                     NRQMELKVEEMNLDLDIGNVYKEKKDLINAGLYFQNGGMLAHQLGDSGLARTFDAALL
                     SLRATRQTQLSAEKRLVGSIQSSIESGDKTSELLSYQYLAEHYAATGEAEKALEYSKR
                     YFEMNDSLQSLNIQLQMKRMESQYNFEKKEREIALLKKDREVSRLSLEKQKIFEYGAV
                     LLCILLVLIGVLVFNRYRVVHRARRLLELEKMRNHIARDLHDDIGSTLSSINILSKVA
                     LQSGDGGESPLRKIMDRSFAIMEKMDDIVWTINPRNDTMEQLVYRMKEFAGEMLEPLN
                     IRYSFEEEGDLVSLKLDIRQRKELYLLFKEAINNAAKYSQCENLHIRLRRAKDFLKME
                     IADDGKGFAEDGQRGGNGLKNMRERAASMPAVIRIDSVVGEGTCIGLDVPVV"
     gene            complement(2206..4416)
                     /gene="katG"
                     /locus_tag="VGQ51_07425"
     CDS             complement(2206..4416)
                     /gene="katG"
                     /locus_tag="VGQ51_07425"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEV9036431.1"
                     /translation="MANSTTQNKCPFHNGTMAQSAGGGARNRDWWPNQLKLSILRQYS
                     SLSDPMDAGFNYAKEFRSLDLDAVKKDIFSLMTTSQDWWPADYGHYGPFFIRMAWHSA
                     GTYRIDDGRGGAGYGTQRFAPLNSWPDNANLDKARLLLWPVKKKYSKKISWADLMILA
                     GNCALESMGFKTFGFAGGRADVWEPAEDIYWGSELEWLGDKRYSGDRDLEIPLAAVQM
                     GLIYVNPEGPNGKPDPLAAARDIRETFRRMAMDDYETVALIAGGHTFGKTHGAAEQSK
                     YVSREPAAANLEEMGLGWKNSFGKGHGADTITSGLEGAWTMTPTKWSNNFFENLFGFE
                     WELTKSPAGAFQWKPKAGAGAETVPDAHDPSKRHAPTMLTTDLALRIDPTYEKISRHF
                     YENPDEFADAFARAWYKLTHRDMGPRSRYLGPEVPAEDLLWQDPIPTVTHPLIDEKDI
                     AALKSKVLTSGLSISQLVSAAWASASTFRDSDKRGGANGARIRLAPQKDWAVNHPAQL
                     SKVLDTLEKIKQEFDQVRPGGKQVSLADLIVLAGCAAVEKAAKNAGYDVTVPFTPGRA
                     DASQEQTDVGSFSVLEPASDAFRNYHGSHAKALPEELLIDKAHLLTLTAPEMTVLIGG
                     MRVLHTNFDGSGHGILTTTPETLTNDFFTHLLDMNTVWQSTSEAAQLFEGRDRATNKV
                     KWTATRTDLIFGSNSELRAIAEVYACEDAKTKFVKDFVHAWAKVMDLDRFDLAK"
     gene            complement(4502..4966)
                     /locus_tag="VGQ51_07430"
     CDS             complement(4502..4966)
                     /locus_tag="VGQ51_07430"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEV9036432.1"
                     /translation="MNPKIDAFLNHAQRWQDEMKRMRTICLDCGLTEELKWGLPCYSF
                     EKKNIVIIQGFKNYCALLFFKGYLLKDPKGILVRTGPNTKVGRQARFTDLKEIIKQAS
                     ALKSCIHEAIEIEKAGVKPAAPKKSAKKAAKTPPPKKTAKKTAKKTAKKSPR"
     gene            complement(4977..>5327)
                     /locus_tag="VGQ51_07435"
     CDS             complement(4977..>5327)
                     /locus_tag="VGQ51_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV9036433.1"
                     /translation="ATLWAALGMVSTALVQLFHLKAKVDFITHLGYPDYFLRIIGAWK
                     ILGAIALLIPKFPLLKEWAYAGCFFVMSGAVMSHIAFGDPFTEMLPSLWLLILIVVSW
                     AFRPADRRIALAHQ"
BASE COUNT         1111 a         1333 c         1624 g         1259 t
ORIGIN      
        1 gtctcggcat gtcaggctat agacataaga cacccggcat atggggatta ctccattttg
       61 tttatcttta tggtatcctc cttctttccc attgagtgtc ttacctgtga tccgcagctt
      121 ctacaaaaaa agccggcatg aaatactttc gtccgatgct actgctgttg ctgcaggcac
      181 tttcgatgct cgttgccgcg cagcctaacg ccaatgacag cctcctggac atcatccgcc
      241 aggacaggcg ggatctatcc gaggcaaggg cgcttaatgc gctggccttc aactatgccc
      301 gaaccgatct ggcgaaggcg aaagggtgtc tgtaccgaag catcgagctg gcgagcgcag
      361 gacatttcgg ggtccagctc tcggctgcct atacccaaat ggtcttttgt cagcaggatg
      421 tgggccaggt ggatagcgcc ggggtatttc tccggctgtt gaaacacctt tccgattcgg
      481 atgccaacct ccggagcaac tatgtgcagg cggcgggcct tttttacaaa cgccagcaga
      541 atttcaaggc cgccatgcct tttttgctgg aagcgctgga tagggcaaag gccgcggccg
      601 cggctgatcc ttctgtggtc aaacgcacgg cggttgcggg cgcattcctg aatgcaggaa
      661 acaactatga cgagcttggg gactaccgga tggcgctctg gaatcacctg gacgctctgc
      721 ggcttttcga agcagtggac aataagaagg gcatttcctt cgcgtatcag accattggcg
      781 gagatttcct tcttctcaac cagctgcaat ccgcctcgga ctataccaaa cggtcattgg
      841 ctatcaagac ggagctcaat gatcagcggg ggatcgctac ctcgctgaaa cagctgggtt
      901 ccgtattccg gtccggcaag cacctggatt cggcgctgga ttattatggc agatcgatcg
      961 cgatcaacag gcagatggag ctgaaggtcg aggaaatgaa tctcgatctt gacatcggga
     1021 atgtctacaa ggagaaaaag gatctgatca acgcgggtct gtattttcaa aatggcggga
     1081 tgctggcgca tcagctgggt gattcgggtc ttgccaggac cttcgatgcc gcgctgctgt
     1141 cgctgcgggc cacgcgccag acacaattgt cggcggagaa aaggctggtc ggcagtattc
     1201 aatcctccat cgaatcgggc gacaagacat ccgaattgct gagctatcaa tacctggccg
     1261 aacattatgc tgccacgggt gaggccgaaa aggcgctgga atacagcaaa agatacttcg
     1321 agatgaacga cagcctccaa agtctgaata tccagctgca aatgaagagg atggagagcc
     1381 aatataattt tgaaaaaaag gaacgggaga tcgcgctgct gaagaaagac cgggaggtat
     1441 cccggttgag cctggaaaag caaaagatct tcgagtatgg ggcggtcctg ctttgtatcc
     1501 tgctcgtatt gatcggtgtg ttggtgttca accgatatag agtggtacac cgggcgagac
     1561 gtctgctcga attggagaag atgcggaatc atattgcgcg ggatctccat gacgacatcg
     1621 gttctacgct ttccagcatc aatatcctga gtaaggtggc attgcaaagc ggggatggcg
     1681 gcgagtcccc tctgcggaag atcatggatc gttcgttcgc catcatggaa aagatggacg
     1741 atatcgtctg gacgatcaac ccgaggaacg ataccatgga acagctggtt taccgcatga
     1801 aggaatttgc gggcgagatg ctggaaccgc tgaatatccg ttattccttt gaagaggagg
     1861 gggacctcgt ctcgctgaag ctggatatcc gtcagcgaaa ggaattgtac ctgctgttca
     1921 aggaggcgat caacaatgcc gccaaataca gccagtgcga gaatctccat atccgtctgc
     1981 ggcgggcgaa ggatttcctt aagatggaga tcgccgacga tgggaaaggg tttgccgagg
     2041 acggacagag ggggggtaac gggttgaaga acatgcgcga acgggcagcc tcgatgccgg
     2101 cggtcatccg gatcgactcg gtggtcggtg agggcacttg tatcgggctc gatgtgccgg
     2161 tggtgtgaaa gcctgcggcg gccgttgccg gcgccgcagg cggtgttatt tggccaggtc
     2221 gaagcggtcg agatccatca ctttcgccca ggcgtgtacg aaatctttta cgaacttggt
     2281 ttttgcgtct tcgcaggcat agacttcggc gatggcgcgc agctcggaat tggagccgaa
     2341 aatgaggtcg gtccgggtgg ccgtccattt taccttgttt gtcgcgcggt cgcggccttc
     2401 gaacagttgt gcggcctcgg aggtggactg ccagacggtg ttcatgtcca gtaagtgggt
     2461 aaagaagtcg ttggtaagcg tttccggcgt cgtcgtcaat atgccgtgtc cggacccgtc
     2521 gaaattggta tggaggacgc gcatgccacc gatgaggacc gtcatctcag gagcggtcag
     2581 tgtcagcagg tgggccttat cgatgagtaa ttcttccggc aatgctttgg cgtgcgaacc
     2641 atgataattg cggaaagcgt ccgaagcggg ttccagtacg gagaaggatc ccacgtctgt
     2701 ttgctcctgc gaggcgtcgg cgcgtccggg agtaaagggg acggttacat cataacctgc
     2761 attcttcgct gccttctcca cggctgcgca acccgcgagg acgatgaggt cggcaagcga
     2821 gacttgtttg ccgccggggc gcacctgatc gaattcctgt tttattttct ccagcgtatc
     2881 gaggactttc gaaagctgtg ccggatggtt gacggcccag tctttttggg gagcgagcct
     2941 gatgcgggcg ccgttggcgc cacctctttt gtcggaatcg cggaaggtcg aggccgaagc
     3001 ccacgctgca gataccagct gcgaaatgga aagaccgctt gtcaggacct tcgatttcag
     3061 ggcagcgata tccttttcgt cgatgagcgg gtgtgtgacc gtggggatcg gatcctgcca
     3121 gaggagatct tcggcgggga cttccggccc gagataccgg ctcctgggtc ccatatcgcg
     3181 atgggtgagt ttgtaccagg cacgggcgaa ggcgtccgcg aactcatcgg ggttttcata
     3241 aaaatgccgg gagatcttct cgtatgttgg atcgatccgg agcgcgaggt cagtcgtcag
     3301 catcgtcggc gcgtggcgtt tggatgggtc gtgtgcatcg ggtacggtct ctgcgcctgc
     3361 gccggccttc ggcttccact ggaatgcacc ggcggggctt ttggtgagct cccattcgaa
     3421 accgaacagg ttttcgaaga aattgttgct ccatttcgtc ggtgtcatgg tccatgcacc
     3481 ttcgagaccg ctggtgatgg tatcggcgcc gtggcctttg ccaaacgagt ttttccagcc
     3541 caggcccatt tcttcgaggt tggccgcagc gggttcccgg ctgacatatt tggattgttc
     3601 ggcggctcca tgggttttcc cgaaggtgtg accgccggcg atgagcgcga ctgtttcata
     3661 atcgtccatg gccatccggc ggaaggtctc gcggatgtcg cgggctgcgg ccagcggatc
     3721 gggttttccg ttaggacctt ccggattgac atagatgagt cccatctgga cggcggcgag
     3781 gggaatctcg agatcgcgat cgccgctata gcgtttgtcg ccgagccact ccagctcgga
     3841 gccccaatag atatcctctg caggctccca tacatctgca cgaccgccgg cgaaaccgaa
     3901 agttttgaag cccatcgact cgagcgcgca gttgccggcg aggatcatca gatcggccca
     3961 ggagatcttt ttactgtatt tcttttttac cggccagagc aggagccggg ccttgtcgag
     4021 attcgcattg tcgggccagc tattgagcgg tgcaaatcgc tgggtcccat aaccagcccc
     4081 gccgcggcca tcgtcgatgc gataggtacc agcgctgtgc caggccatcc ggatgaagaa
     4141 tgggccataa tggccgtagt ctgccggcca ccagtcctgc gaggtagtca tcaggctgaa
     4201 gatatctttc ttcacggcgt ccaggtcgag gctcctgaat tctttggcgt agttgaagcc
     4261 ggcatccatc ggatcggaga gagaggagta ttgacgaagg atgcttagct tcagctgatt
     4321 gggccaccag tcgcggtttc tcgcaccgcc gcctgcgctt tgcgccatcg ttccgttatg
     4381 gaacgggcac ttgttctgag tcgtggaatt tgccatatgg aaatgattga gagcgcgaag
     4441 ctactgagat ggcggttgcc gggcaaattg attgttttta tgatcagata gttattatct
     4501 atcagcgggg agattttttg gcggtttttt tggcggtctt ctttgcggtc tttttgggag
     4561 gtggtgtttt cgcggccttc tttgccgatt ttttgggcgc cgccggtttc actcctgctt
     4621 tttcgatttc gatggcttcg tggatacagg attttaaggc ggaggcctgc tttatgatct
     4681 ccttgaggtc ggtgaagcgt gcctggcggc ctaccttggt attggggccg gtcctgacca
     4741 ggatgccttt ggggtctttc agcaggtagc ctttgaagaa caggagggcg cagtaattct
     4801 taaagccctg gatgatgacg atattctttt tttcgaagga gtaacaggga agtccccatt
     4861 tcaattcttc ggtgagaccg caatcgagac agatcgttct catccgtttc atttcatcct
     4921 gccatctttg ggcgtggttc aggaatgcat cgatcttggg attcatggta ttcgttttat
     4981 tggtgagcta aagcgatcct tctgtccgcg ggtctgaagg cccaagatac gacaatcagg
     5041 atcagtaacc acagcgaggg caacatctct gtgaaaggat ccccgaaggc gatgtgtgac
     5101 attaccgctc ccgacatgac gaagaagcaa ccggcatagg cccactcctt gagcaagggg
     5161 aatttgggga tcagcagggc gatggcgccg aggatcttcc aggcaccgat gatcctcagg
     5221 aaataatcgg ggtagcccag atgggtaata aaatccacct ttgctttcag gtggaagagc
     5281 tggacgagcg cagtagacac cattcccaga gcggcccaga gagtggc
//
LOCUS       DASYSV010000241         7763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1197054, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000241 DASYSV010000000
VERSION     DASYSV010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197054"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /gene="guaA"
                     /locus_tag="VGQ51_07440"
     CDS             <1..718
                     /gene="guaA"
                     /locus_tag="VGQ51_07440"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV9036434.1"
                     /translation="GIDAKQRFYTELAGKTDPEVKRKTIGRLFIEVFQEEAKKVEGVE
                     LLGQGTIYPDVIESVSVHGPSVTIKSHHNVGGLPEKMHLGLVEPLRFLFKDEVRRVGA
                     ELGIPEDLLQRHPFPGPGLAIRILGEVTEEKVRLLQEADHLYIRGLKEHGLYSAVWQA
                     GAILLPVKSVGVMGDERTYEFTVALRAVTSVDGMTADWAHLPYTFLADISNDIINGVK
                     GINRVVYDISSKPPATIEWE"
     gene            742..1965
                     /locus_tag="VGQ51_07445"
     CDS             742..1965
                     /locus_tag="VGQ51_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036435.1"
                     /translation="MNKNLRYSLLVLCLCLVGTIAATAQVDTTRRISDTMRQHPDTSR
                     RKLVILAPLYLDSAFDATGSYRYDKSFPKFINPGLEFYEGIQMALDTMQAEHAQLTVQ
                     VSDTRSSAMSLSQMLDSTSLQGVGLIIGLVDNARELQQMASAADRQHIPFINANYPND
                     GGITNNPSLVILNSTLRTHCEGIYRLLQRNYPTKPLIFFRRKGAQEDRLKGYFTDIDK
                     TTAGVPLKIKYVTLDDGFDAAALAANLDSAEQSVCIAGSLDESFGYKLCALLATLKSY
                     HTTLVGMPTWDNLDWTRPECAGEEIIYSTPFYINPSDTLVKIISQEFKTKFYSRPSDM
                     VFRGYECAYRFGKLLMLRGGGLNGSVGEKKFKVFNDFEILPVFLNRKTMTLDYFENKK
                     LYFIHKTDGNITSVE"
     gene            2077..3204
                     /locus_tag="VGQ51_07450"
     CDS             2077..3204
                     /locus_tag="VGQ51_07450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEV9036436.1"
                     /translation="MPLIESTENGLYCAQGQFYIDPWRPVDKAVITHAHSDHARAGSA
                     SYLCHRLTLPLLELRLGPHAYQAVEWGEVVYLNGVRVSLHPAGHIIGSSQVRVEHRGE
                     VWVASGDYKTEDDGLSGAFEAVPCHVFITESTFGLPIYNWKPQPELLVSMEEWVRTNA
                     AKDRTSVLIAYSLGKAQRVLAGLAHVVQPIYLHGAVYNVHTALQRAGIPLPEALRVMP
                     DMPKGLFRGKLVIAPPGAEGSSWMRRFGEYALGICSGWMQVRGNVRRRNADAGFAMSD
                     HADWKGLLAAVDATGAERLLVTHGFQAVLARYLSEKGIEAGEVRTEYGEDEETAGAAT
                     EESDSAASGLDSAAGEGGLDSATEEGGLDSATGEGAANGQD"
     gene            3215..4846
                     /locus_tag="VGQ51_07455"
     CDS             3215..4846
                     /locus_tag="VGQ51_07455"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEV9036437.1"
                     /translation="MRRFATLALQLGTSTKTNDKLEALEAYFREAADADKVWVIALFS
                     GRRPRRAVTTTQLWEWCRELTGLPAWLFEESYHTVGDLAETIALLLPEDAQVENRQSL
                     AHYLQRLMRLGAEDDTGKKAFILDCWATMNKDERFVFNKLITGGWRIGISQQTVVNAL
                     ARTTAEEASVVAHRISGDWDPGSTSFAALLSGGGEDLSRPYPFYLAYALEDDLSAPGR
                     PDEWQAEWKWDGIRGQLIRRGDRLYLWSRGEELITDKFPEYQDFLQRLPAGTVLDGEI
                     IGLGSRAVAGALPFVVLPFAALQTRIGRKNISKKHLNEVPVGFIVYDLLEFGGEDWRN
                     KPLTDRRRQLESIVTAAAHPLLQLSPVIAFDNWEQLAAIRATAREHGSEGIMLKRKTS
                     IYQVGRKRGDWWKWKIDPLVIDAVLVYAQKGHGRRSNLYTDYTFAVRAGDQLMTFAKA
                     YSGLTDKELAEVDAFVKKNSLEKFGPVRTVKPELVFEIAFEGIAASSRHKSGVALRFP
                     RIARWRRDKGPEDIDTLDTLKKMLAMYGKQTPSAT"
     gene            4935..6314
                     /locus_tag="VGQ51_07460"
     CDS             4935..6314
                     /locus_tag="VGQ51_07460"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036438.1"
                     /translation="MNKLTVLILLGVMGLAFTTGLEISRIEQPVPVPASPQRRGDATA
                     GYHYLTTGDYIKSGIPYNFFLLGYGKRPDTLLHRDGPNGDIRYDYTAVKAANGVEVVA
                     PNCLQCHAQVFDGKLIMGLGNTSIDFSDARKFEGSATGAIERLLQKNPSQFEAAAPFL
                     TSLKAIGDGIRTEVRGVNAADRLTGLLVAHRDPETLRWSETPNMEITGRVVPTDVPAW
                     WLLKKKNAMFYNGFGRGDFGRFLMASSLLTLSDSSEAREVDNHIPDVLAYLYTLRPPK
                     YPYPIDETLARRGGVLFVEHCSKCHGNYGDGGDYPNLLIPQNIIGTDSLLNKNDYQSP
                     QIVDWFNKSWFARGDHPARLEPFDGYIAPPLDGIWITAPYLHNGSVPTLEGVLNSKRR
                     PAYWSRDFDHPQYDYAAVGWKYTRETGARGTAVYNTTLPGYGNQGHSFGDKLSDRDRK
                     AIIEYLKTL"
     gene            6407..>7763
                     /locus_tag="VGQ51_07465"
     CDS             6407..>7763
                     /locus_tag="VGQ51_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV9036439.1"
                     /translation="MQLKETIGYTTIAAWMEAQGREPFAFQEEAWQHILEGRSGLVNA
                     PTGCGKTFSVFLGALISFINHHPDDFRNRSGLGLQLLWISPLRALAKDIGRAMEEVIT
                     GLGMRWKVGLRNGDTDVSERQKQKRRMPEVLIITPESLHLLLAQKGYPETFKTLKIVA
                     VDEWHELLGSKRGVQVELALSRLAAMRGTEVSFIGISATIGNLEQAKEVLLAPLDRAG
                     GGVESDGRGTSGGGEAFGGGGESGGGEAGFIVRANIDKSIEVESILPDEIEKYPWAGH
                     LGLRLIHKVIPILETSRTTLIFINTRGMSETWYQSLLNICPDLAGAIALHHGSIDMAL
                     RNWVEDALHEGKLKAVVCTASLDLGVDFRPVETVIQVGSPKGVARFLQRAGRSGHQPG
                     QVSKIWFLPTHSLELMEAAALKTAMKEGLIENREPMLECFDVLIQYLSTLAISDGWYP
                     ER"
BASE COUNT         1746 a         1985 c         2456 g         1576 t
ORIGIN      
        1 aggtatcgac gctaaacaac ggttctatac cgagctggcc ggcaaaacgg atcccgaagt
       61 caagcgaaag acgatcggaa gactctttat tgaagtcttc caggaagagg caaagaaagt
      121 agagggtgta gaattgctgg gacagggcac tatttatccc gatgtcatag aaagcgtgag
      181 cgtacacggt ccgtctgtga ccatcaaatc ccatcataat gtcggcgggc tgcccgaaaa
      241 gatgcatctg gggctcgtag agccgctgcg ttttttgttc aaggacgagg tcaggcgagt
      301 aggcgcggaa ttaggcatac cggaggacct tttacaacga catcctttcc ccggtccggg
      361 attggcaatc cgcatcctcg gcgaggtcac ggaagaaaag gtgcgattat tgcaggaagc
      421 agatcatctc tatatccggg ggctgaagga acacggactg tattcggcag tttggcaggc
      481 gggggcaatc ctgctgccgg tgaagagcgt aggggtcatg ggtgacgagc ggacctatga
      541 attcacggtg gcccttcggg ccgtgacctc ggtcgacggg atgacggcgg actgggctca
      601 tttgccgtat acctttctgg cggatatctc caatgacatc atcaatggtg tcaaagggat
      661 caaccgggtg gtgtatgaca tcagcagcaa gccaccggcg acgatagagt gggaataacc
      721 gccgtcgcgg taccatgcga tatgaataaa aacctccgat attctttgtt ggtgttgtgt
      781 ttgtgtttgg tgggaaccat cgccgctacc gcgcaggtag atacgaccag gcgtatttcc
      841 gataccatgc gtcagcaccc ggacacttcg cgccgcaagc tggtgatctt agcccctttg
      901 tatctcgatt cggcgttcga tgccaccggc agctaccgct acgacaaaag tttccccaaa
      961 ttcataaatc ccggcctgga attctatgaa ggaatacaga tggcgctgga tacgatgcag
     1021 gccgaacacg cccagctcac cgtacaggta tccgataccc ggtcgtcggc aatgagtctg
     1081 tcgcagatgc tggacagcac atctctccag ggtgtcgggc tgatcatcgg gctggtggac
     1141 aatgcccgcg agctccagca aatggcttcc gcggccgacc gccagcatat tcctttcatc
     1201 aacgccaact atcccaacga cggcggaatc accaacaatc cgagtctggt catcctcaac
     1261 tcgaccctgc gcacgcattg cgaaggcatc tatcgtctcc ttcaacgcaa ctatcccacc
     1321 aaaccgctga tctttttccg ccggaagggc gcacaggaag acaggctgaa gggttatttc
     1381 acggacatcg acaagaccac cgcgggagtg ccgctcaaga tcaaatatgt caccctggat
     1441 gacggtttcg acgccgcggc gctggctgcc aatctcgaca gcgcggaaca gagcgtctgt
     1501 atcgccggct cgctggatga gtccttcggc tataagcttt gcgccttgct ggccacgctc
     1561 aagagctatc acacgaccct ggtcgggatg cccacctggg acaatctcga ctggacgaga
     1621 ccggaatgcg cgggtgagga gatcatttat tctactcctt tttatatcaa tccttcggat
     1681 acgctggtca agatcatctc gcaggaattc aagacaaaat tttattcccg gcccagcgat
     1741 atggtgttca ggggatacga atgtgcatat cggttcggca aattgctgat gttgcgcgga
     1801 ggcggtctaa acggcagcgt cggcgaaaag aagttcaaag ttttcaatga tttcgagatc
     1861 ctgccggtat tcctcaatcg taaaacgatg acgctggact atttcgagaa caagaagctg
     1921 tatttcatcc ataagacgga tgggaatatt acttcggtgg agtgagcgac ggcgaagata
     1981 ttcgacggtg ggatggacgg acgacggaga taatccacgg tggagggacg gacgacggag
     2041 atattccacg gtgatgcagc agacaaatcg gtttctatgc cgttgatcga atctacagag
     2101 aacggccttt attgcgcgca gggacagttc tacatcgacc cctggcgccc ggtggacaaa
     2161 gccgtgatca cgcatgccca tagcgatcat gcgcgggcgg gcagcgccag ttatctctgt
     2221 caccggctta cgcttccttt attggaatta cgattggggc cgcatgcata ccaggcggtg
     2281 gaatggggcg aggtcgtata cctgaacggc gtgcgggtca gtctgcatcc cgccggtcat
     2341 attatcggat cgtcgcaggt gcgtgttgag caccggggag aggtatgggt cgccagcggc
     2401 gattacaaga cggaagacga cggactcagc ggtgcattcg aagccgtccc ctgtcatgta
     2461 ttcattaccg agtctacctt cggattgcct atctataact ggaagcccca gccggagcta
     2521 ttggtctcca tggaggagtg ggtccggacg aatgcggcca aggacaggac ctccgtgctc
     2581 atcgcctata gcctgggcaa ggcccaacgg gtgctggcgg gtctcgcgca tgtagtgcaa
     2641 cccatctatc ttcatggtgc ggtctacaat gttcacactg cgctgcagcg tgcgggtatc
     2701 ccgctgccgg aggcgctacg tgtcatgccc gacatgccga aagggctttt ccgcgggaaa
     2761 ttggtgatag cgccgccggg cgccgaaggt tcttcctgga tgcggcgttt cggcgaatat
     2821 gcgttgggca tctgcagcgg gtggatgcag gtgaggggaa atgtgcgccg gcgaaatgca
     2881 gatgcgggat tcgctatgag cgatcatgcg gactggaaag ggttgctggc ggcggtcgac
     2941 gcgaccggcg ccgaaaggct gctggtgacg cacggattcc aggccgtgct ggcgagatat
     3001 ctcagcgaaa agggcatcga ggcgggggaa gtccgcacgg aatatggaga ggatgaagag
     3061 acggcgggcg cggcgacgga ggagtcggat tcagcggcga gcgggttgga ttcggcggcg
     3121 ggggagggcg ggttggattc agcgacagag gaaggcgggt tggattcagc gacgggggag
     3181 ggcgcggcga atggacaaga ttgataccgg cgggatgcgg cggttcgcca ccctggcgtt
     3241 gcagctgggc accagtacga agaccaatga caagctggag gcccttgaag cgtatttccg
     3301 ggaggccgcc gatgccgaca aggtatgggt gatcgcgctg ttcagcgggc ggcggccgag
     3361 gcgtgcagta actactacgc agctatggga atggtgccgc gagctgacgg ggttaccggc
     3421 atggctattc gaagagagct atcacaccgt aggcgatctt gcggagacta tcgcgttgtt
     3481 gctgccggaa gacgcccagg tggagaatcg ccaaagtttg gcgcattacc tgcagcgtct
     3541 gatgcggctg ggcgccgaag acgacaccgg gaagaaggca tttatccttg actgttgggc
     3601 gaccatgaac aaagacgagc ggtttgtctt caacaagctg atcaccggcg gatggcgcat
     3661 cggcatttca caacagacgg tcgtcaatgc actggcgagg acgacggcgg aggaggcatc
     3721 ggtggtggct catcgaatca gcggggactg ggacccggga tcgacttctt ttgcggcgtt
     3781 gctcagcggc ggcggtgaag acctctcgcg gccctaccct ttttacctgg cctacgcatt
     3841 ggaagatgat ctgtccgcgc ctggccgtcc cgacgagtgg caggcagaat ggaaatggga
     3901 tgggatacgc gggcagctga tccgcagagg cgatcgtctc tatttatgga gtcgcgggga
     3961 agagctgata acggataaat ttccggaata ccaggatttc ttacagcgtt tgccggcggg
     4021 gaccgtgctg gacggggaga tcatcggact tggaagcagg gcggtggccg gcgcgttgcc
     4081 cttcgttgtc ctccctttcg cggccttgca gacccgcatc gggcgaaaga acatctccaa
     4141 aaaacatctc aacgaagttc cggtgggctt tatcgtctac gatttgctcg aattcggcgg
     4201 ggaagactgg cggaacaagc cactgacaga ccggagacgt cagcttgaat ccatcgtgac
     4261 agcggcagcg cacccgctgt tacaattatc gcccgtcatc gcattcgata actgggaaca
     4321 gttggccgcg atccgggcca cggcccggga gcatggcagc gaaggcatca tgctcaagcg
     4381 aaaaacctcc atctaccagg taggccgaaa aagaggcgac tggtggaaat ggaagatcga
     4441 tccgctggtg atcgatgccg tgctcgtcta tgcgcagaag ggtcatggcc gccgcagcaa
     4501 cctttataca gactatacct tcgccgtgcg ggcgggagat cagctgatga cgtttgcgaa
     4561 agcctattcg gggctcaccg acaaagagct ggccgaggtg gacgccttcg tcaaaaagaa
     4621 ctcgctggaa aagttcgggc cggtgcgaac cgtcaagccc gagctggtct ttgaaatagc
     4681 cttcgaaggt attgccgcat cgtcgcgaca taagtcgggg gtcgcgttgc gtttcccgcg
     4741 gatcgcccgc tggagaaggg ataagggtcc cgaagatatc gacaccctgg atacgctgaa
     4801 gaaaatgctg gcgatgtatg ggaagcagac tccgtcggcg acatgaagaa gatgcaggag
     4861 atctgcggga agcagactcc gccggcgcca tgaagaagat gcttgagatc tgggggaagt
     4921 agtaaattta cagtatgaac aagctgacgg tgctgatact gctgggcgtg atgggactgg
     4981 cgttcacgac gggtttggag atctcgcgga tagaacagcc ggttcccgtt ccggcgagcc
     5041 cgcaacgaag gggagatgcg acggcaggtt atcactacct gaccacgggt gattatatca
     5101 aaagcggtat tccctacaat ttttttctgt tgggttatgg aaaacgaccc gacacccttc
     5161 tccaccgcga tggtccgaat ggggatatcc ggtatgacta tacggctgtc aaagcagcga
     5221 atggagtgga ggtggttgct ccaaactgtc tgcaatgtca cgcacaggtg ttcgacggca
     5281 agttgatcat ggggttggga aacacatcga tcgatttttc cgacgcacgg aaattcgagg
     5341 gcagcgcgac cggcgcgatc gaacggctat tacaaaaaaa tccttcccaa ttcgaggcgg
     5401 cggcgccttt tcttacatcc ctgaaggcca tcggtgacgg gatccggacg gaggtgcggg
     5461 gggtgaatgc ggccgacagg ctgacagggt tgctggtcgc acatcgtgat ccggagacct
     5521 tgcgctggag cgagaccccg aacatggaaa taacaggccg tgtcgtgcct accgatgtgc
     5581 cggcgtggtg gctactgaag aaaaagaatg ccatgttcta caatgggttt ggccgaggcg
     5641 atttcggaag attcctgatg gcgtcgagcc tgctcacgct tagcgatagt tccgaggcgc
     5701 gcgaagtgga caatcatatc cctgacgtgc tcgcctatct ttacaccctg cggccgccga
     5761 aataccccta cccgatcgat gagacgctgg ccagacgggg cggggtactt ttcgtggagc
     5821 attgttccaa atgtcatggc aactatggcg acggcggaga ctatcccaac ttattgatcc
     5881 ctcaaaacat cattgggacg gattcgcttt tgaataaaaa cgactaccag agtccgcaga
     5941 tcgtggactg gttcaacaaa agctggttcg cgcggggcga tcatccggcg cggctggagc
     6001 cattcgatgg ctatatcgct cctcccctgg acggcatttg gatcaccgct ccctatctgc
     6061 ataatggttc cgttcccacg ctggaaggag tgctgaacag caagagacgg ccggcgtatt
     6121 ggtcgcgcga tttcgatcat ccgcaatatg actatgccgc agtcggatgg aagtataccc
     6181 gtgaaaccgg cgcccgcggc acggcggtct acaatacgac gcttccggga tatggtaatc
     6241 agggacacag cttcggcgac aaactttccg atcgcgaccg gaaagccatc atcgaatatc
     6301 tgaagacgtt gtagcgacga accgacgcgg gcgactatta cgcatagggg tcattttatc
     6361 atgagacttt gtaatttaga taggggttaa gtgaggaaca attaaaatgc aattaaagga
     6421 gacgataggt tataccacta ttgcagcgtg gatggaagct caggggcgtg agccttttgc
     6481 ttttcaggaa gaggcgtggc aacatatcct ggagggtcgg tcgggcctgg tgaatgcgcc
     6541 gacggggtgc gggaagacct tttccgtctt cctcggagcg ttgatctcat tcatcaacca
     6601 tcatccggac gactttcgta acaggagcgg tctcggactg cagctgttgt ggatatcccc
     6661 tttgcgcgcg ctggcaaagg atatcgggag ggccatggag gaggtgataa ctgggttggg
     6721 tatgcgctgg aaggtgggac tgcgcaacgg cgacacggac gtcagcgagc ggcagaagca
     6781 gaaaagacgg atgccggagg tgctgatcat tacacccgag agtctgcacc tgttgctggc
     6841 tcagaagggt tatcccgaaa cattcaagac cctgaagatc gtcgccgtgg atgagtggca
     6901 tgagctgttg gggagcaagc ggggtgtgca ggtggagctg gcgttgtcgc ggctcgccgc
     6961 gatgcgcggc acggaggtgt cgttcatagg aatcagcgcg accatcggaa acctggaaca
     7021 ggcgaaagag gtgctgctcg cgccgttgga tagggcgggc ggcggtgtag agtcagatgg
     7081 gcggggaaca tccggtggcg gcgaagcatt tggcggcggc ggagaatccg gtggcggcga
     7141 agcgggcttc atcgtccgcg ccaatatcga caaatccatc gaagtcgaat ccatcctgcc
     7201 cgacgaaata gaaaaatatc cctgggccgg tcatctcggt cttcggttga tccacaaagt
     7261 gatccccata ttggaaacca gccggacgac actcatcttc atcaacaccc gggggatgag
     7321 cgagacctgg tatcagagcc tgctcaatat ctgccccgac cttgccggcg cgatcgcgtt
     7381 gcatcatggt tccatcgaca tggccttacg caactgggtg gaggacgcgc tgcatgaagg
     7441 aaagctgaag gcggtggtct gtaccgccag tctcgatctg ggggtggact tccggcccgt
     7501 ggagacggtc atccaggtcg ggagtcccaa aggggtggcg cgtttcctgc agcgcgccgg
     7561 gcgcagcggg caccagccgg gccaggttag caagatatgg ttccttccga ctcactcgct
     7621 cgaattgatg gaagccgcgg cattgaagac agccatgaag gaagggttga tcgagaaccg
     7681 cgagccgatg ctcgagtgtt tcgacgtgct gatccagtat ctgagcacgc ttgccatctc
     7741 cgacgggtgg tatcccgagc ggc
//
LOCUS       DASYSV010000242         6348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1324622, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000242 DASYSV010000000
VERSION     DASYSV010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324622"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1760
                     /locus_tag="VGQ51_07470"
     CDS             <1..1760
                     /locus_tag="VGQ51_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV9036440.1"
                     /translation="SGKSTLLRILSWKDTADEGTVWIHKDVTVALFEQEPSFIEDRSI
                     LDNIFHHDHPIINAIKVYEDASETADADRLTKAIVRMDELGAWDFDSRVKQILGKLNI
                     HHLHQSAGTLSGGQRKRVALAKTLIDIGFEHKHVLLIMDEPTNHLDVEMVEWLEHYLN
                     QEKVTLLLVTHDRYFLDAVTEEIWEMDGSDLYVYKGDYQHYLEKKTSRIESETASIDK
                     ARNTYRKELEWMRKQPKARTTKSKSRQDNFYAVEARAKQRIEDQQVELQMKMNRLGGK
                     VAELKKVYKSWGDKIILQGFDYTFKRGERVGVIGKNGVGKSTLINILQGLEQADSGKV
                     NIGETVIFGNYSQQGLVIKEDMRVIEFVKDIAEHFPLAAGGSLSAAQFLNLFLFPPEQ
                     QYTYISKLSGGEKRRLHLLSILFRNPNFLVLDEPTNDLDLPTLGILENFLSEYEGCLL
                     IVSHDRYFMDRLVDHLLVLEGDGVIRDFPGNYSQYRDSLKEQETGEITPKEPAKPAAA
                     PTPSSRRQLTYKEKREFEGLEQEIARLSLEKREVTEKLNSGAVPFDQLQQLSIRIGEI
                     SQLLDDKELRWLELSELVG"
     gene            1852..3372
                     /locus_tag="VGQ51_07475"
     CDS             1852..3372
                     /locus_tag="VGQ51_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV9036441.1"
                     /translation="MRKIFIAASLCFAFAAQAQKIPDARPYAKTITADDLKKHLYIVA
                     SADFEGRETATEGQHKVADYIENHFKSLGLQPGNGDHYKLPYPVYQDSLESASLSVAS
                     QAFALNQDFFAPVASNNSLTLLGSEVVFAGYGVDDSTRNDYKDLNVRGRIVVVLNGNP
                     PGTPAPTGRRRFGPTKQDFAQRNGAAAVLILYKGDRVPPQLSAPRGNMSVTEFRRTNS
                     IDNFYITEKVARAIFGDDYGKLESGTPSPKVYTADIKMSLQKITHELQASDVLGFLPG
                     TDLKDQIVVISAHMDHLGKRNGEIYYGADDDGSGTVSVLELASAFAKARDAGKGPRRS
                     ILFLANSGEEEGLWGSAFYTDHPTYPLDHTTVDLNIDMIGRIDPHRNYGDSMNYVYVV
                     GSDKLSTDLKPISEGTNKKYTKLELDYKFDDPKDPERIYYRSDHFNFARKGVPIIFYF
                     DGIHADYHRPTDTPDKIDYDLLAKRGQLVFYTAWDMANRNDMLKRDIPLPATAATR"
     gene            complement(3369..4346)
                     /locus_tag="VGQ51_07480"
     CDS             complement(3369..4346)
                     /locus_tag="VGQ51_07480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV9036442.1"
                     /translation="MQDEKLQMFRNRLTKDYRHIGRLARRQGIGCYRIYDHDLPEFPF
                     CIERYEDKLYLAEYQRRHGMTDEVHAEWLESCMPVISEVLETPDEKIYYRQRKRKAGR
                     QDQYEKLASEEEFFIVREAGLSFRVNLTDYLDTGLFLDHRITRGMLRAESAGKRVLNL
                     FCYTGSFSVYAAAGGAARVDSVDLSKTYLAWAGENMALNAPVEQHRFIHADVKQWLEE
                     VPAAEYDLVVMDPPTFSNSKRMKDFLDIQRDHAELINQTLRAMVAGGVLYFSTNYRSF
                     QLEAEKIAGAEIKNITNATTPFDFQGRLWRQCYRIIKERPYGPFGIIKA"
     gene            complement(4336..5559)
                     /locus_tag="VGQ51_07485"
     CDS             complement(4336..5559)
                     /locus_tag="VGQ51_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9036443.1"
                     /translation="MLTRTFSLYKKAYSGLSRETWLLSLVMLINRSGTMVFPFMTIYL
                     TQPTMGYTIGQAGLVIGIFGLGAMCGGWLGGRMADRIGFHRVQLVTLTGGGFLFILLG
                     QMKSYPLICICSFLLSVVNDAFRPANSTAIAAYSKEENRTRSFSLNRLAVNLGWAVGG
                     AIGGILASINFHLLFWVDGVTNLLAALLLRMFLAPSSASTPGDRINNGEGPALSPYRD
                     RIYLLFIAGTILFGSCFFQMFSVLPVFYKKMLGMPEYMIGLLMTLNGLLITFFEMVLV
                     FKLEGRREVLYYISCGVLFVGASYLLLNVFPLSVYTAYFCMILMTAGEITAMPFMNSF
                     WISRTAASNRGQYAGLYTIAWSTAQVVGPAGGAEIAQHWGFRPLWWLVCGVCLLAAAG
                     FRRLLQQIKPVVHAG"
     gene            complement(5628..>6348)
                     /locus_tag="VGQ51_07490"
     CDS             complement(5628..>6348)
                     /locus_tag="VGQ51_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEV9036444.1"
                     /translation="TVVTFSLFSVVIVNAQSSLLDKAKSAANSLKGGKGLSSDDIVAG
                     LKDALNHGTQHSTDKLSAVDGFFKDAAVKILLPPDAVKVEKTLRSAGFGKQVDDAILS
                     INRAAEDAATKAAPIFLDAIRNMTVSDGLSILRGSDSAATAYLRKSTSAQLTAAFLPV
                     IKASLEKTGATKYWKDIFDTYNKFPTTFHKVNPDLPSYATDRALYGVFFYVAVEEKNI
                     RQNPAARVDDLLKKVFGSGTR"
BASE COUNT         1612 a         1950 c         1514 g         1272 t
ORIGIN      
        1 gcagcggtaa atctaccctg ctgcgcatac tctcgtggaa agacaccgcc gacgagggca
       61 cggtctggat acataaagat gtcaccgtcg ctttgttcga gcaggagcct tccttcattg
      121 aagaccggtc catcctcgac aatatcttcc accacgatca tcccataatc aatgccatca
      181 aggtctatga agacgccagc gagacggccg acgccgaccg cctcacgaag gccatcgtca
      241 ggatggatga gctgggtgcc tgggatttcg attcccgggt taaacagatc ctcggcaaac
      301 tgaacatcca ccatctccat caatcggccg gcacgctgtc cggaggacaa aggaaacgcg
      361 tggccctcgc caaaaccctt atcgacatcg ggttcgaaca caaacacgtc ctccttatca
      421 tggacgagcc gaccaaccat ctcgatgtgg aaatggtgga atggctcgag cattacctca
      481 accaggaaaa ggtgaccctc ctgctcgtga cccacgaccg gtacttcctc gatgccgtca
      541 ccgaagagat atgggaaatg gatggcagcg atctctatgt gtataaagga gattaccaac
      601 actacctcga aaaaaagacc tcccgtatcg aaagtgaaac ggcttctatc gacaaggcac
      661 ggaataccta tcgcaaagag ctggaatgga tgcgcaaaca acccaaggca cgcaccacga
      721 aaagcaagag ccgccaggat aatttctatg ccgtggaagc ccgcgccaaa caacgcatcg
      781 aagaccagca ggtcgagctc cagatgaaaa tgaaccggct gggtggcaag gtggctgagc
      841 tgaaaaaggt ctacaagagt tggggagaca agatcatcct tcagggcttc gactatacct
      901 tcaaacgagg agaacgggta ggcgtcatcg ggaagaacgg cgtcggcaaa tccactctta
      961 tcaacatcct tcagggcctc gaacaggccg acagcgggaa ggtcaatatc ggcgaaaccg
     1021 tcatcttcgg gaattattca caacagggtc tggtcatcaa agaggacatg cgggtcatcg
     1081 aattcgtcaa agacatcgcc gaacattttc cgctggcagc cggcggctcg ctcagcgcgg
     1141 cccaattcct caatctcttc ctcttccctc cggaacaaca gtatacctat atttctaaac
     1201 tcagcggcgg cgagaaacgt cgcctccacc tgctgtccat ccttttccgt aatccgaatt
     1261 tcctggtgct cgacgagcct acaaatgatc tcgacctccc cacgctcggc atactcgaga
     1321 attttctcag cgaatacgaa ggctgcctgc tcatcgtgtc gcacgaccgt tattttatgg
     1381 accggctcgt ggaccatctc ttagtactcg aaggagacgg cgtcatccgc gatttccccg
     1441 gtaactattc tcaataccgc gactcactga aagaacagga gaccggcgag atcaccccta
     1501 aagaacccgc aaaaccggcc gctgccccga ccccatcctc ccgacgccag ctgacatata
     1561 aagaaaaacg agaattcgaa ggcctggagc aggaaatcgc ccggctctcg ctggagaaac
     1621 gcgaagtcac ggaaaaattg aacagcgggg ccgttccttt cgaccaatta cagcaactat
     1681 ccattcgcat cggcgagatc agccaactcc tcgacgacaa ggaactccgc tggctcgagc
     1741 tcagcgagct ggtgggctga atacccggac ccgaactgaa tttgttcatc cgctcatcca
     1801 aaaatccggc cctccggcac gaaataagca ccggggcgtt atatttggtc catgagaaaa
     1861 atctttatcg cagcgtctct gtgcttcgcc tttgctgcac aggcccaaaa aattccggac
     1921 gcgcgtccat acgcgaaaac catcaccgcc gacgatctga aaaagcacct gtacattgta
     1981 gcgagtgccg atttcgaagg ccgtgagacc gctaccgaag gtcagcacaa agtagccgac
     2041 tacatcgaaa atcatttcaa atcgttggga ctccaacccg gtaacggcga ccactacaag
     2101 ctgccctacc ccgtgtacca ggattcgctg gaaagcgcat ctctctcggt cgccagccag
     2161 gcattcgcgc tcaatcagga tttcttcgct cccgttgcct cgaataacag cctcacctta
     2221 ctgggaagcg aggtcgtctt cgccggctat ggtgtagacg actcaacacg caacgattac
     2281 aaagacctta atgtccgtgg ccgcatcgtc gtggtgctca atggcaaccc tcccggtacg
     2341 ccggcgccca ccggccgtcg ccgcttcggt ccgaccaaac aggatttcgc tcaacgtaac
     2401 ggcgcggcag cggtcctgat cctgtacaag ggcgatcgcg ttccgcctca gctgagcgcg
     2461 ccgagaggga atatgtccgt caccgaattc cgtcgcacca attcgatcga taatttttat
     2521 atcaccgaga aagtcgcccg cgcgatattc ggcgatgact atggtaagct cgaatccggt
     2581 actccctcgc cgaaggtcta taccgctgat ataaaaatgt cactgcagaa aataacgcac
     2641 gagctccagg ccagcgatgt cctcggcttc ctgccgggca cggacctgaa agaccagatc
     2701 gtcgtcatct cggcgcacat ggaccacctc ggaaaacgca acggtgagat atactatggc
     2761 gccgacgacg atggctccgg aacggtcagc gtcctcgaac tcgccagcgc cttcgccaaa
     2821 gcacgcgacg ccggtaaagg cccccgccgc agcatcctct tcctggccaa ctccggagaa
     2881 gaagaagggc tctggggctc cgcgttctat accgaccatc cgacctaccc attggaccat
     2941 accaccgtcg atctcaacat cgatatgatc ggtcgcatcg acccgcaccg caattatggc
     3001 gactcgatga actatgtgta tgtggtaggc agcgacaagc tttccaccga tctcaaaccg
     3061 atcagcgaag gcaccaacaa aaaatatacg aaactggaac tggactataa gttcgacgac
     3121 ccgaaagatc cggaacgcat ctactaccgc agcgaccatt tcaacttcgc ccgcaaaggc
     3181 gtccccatca tcttctattt cgacggtatc catgcggact atcaccgtcc taccgataca
     3241 ccggataaga tcgattacga tctcctcgcc aaaagaggac agctggtctt ctatacggcc
     3301 tgggacatgg ccaaccgcaa cgacatgctc aaacgcgata ttccgttgcc ggcaacggcc
     3361 gcgacccgct aagctttaat gataccgaaa ggcccgtagg gcctttcctt tataatgcga
     3421 taacactgac gccacaatct gccttggaaa tcgaaggggg ttgtggcgtt tgttatattt
     3481 tttatctccg ccccggcaat cttttctgcc tccagctgaa agctccggta attcgtactg
     3541 aagtacaaca cgccgccggc aaccatcgcg cgcagcgttt gattgatcaa ctccgcatga
     3601 tcgcgctgga tatcgaggaa gtccttcatc cgtttgctat tactgaaggt cggcgggtcc
     3661 atgaccacga gatcatactc cgccgccggc acctcctcca gccattgttt gacatcggca
     3721 tgaatgaacc gatgttgctc aaccggggca ttcaacgcca tattttcccc agcccaggcc
     3781 aggtaggtct tcgacagatc gaccgaatcc accctcgccg ctccgcccgc cgccgcatat
     3841 acagaaaatg accccgtata gcaaaacagg ttgagtaccc ttttaccggc agactcggcc
     3901 ctcaacattc ctctcgtgat acgatgatcc aaaaacaatc ccgtatcgag atagtccgtc
     3961 aggttgaccc ggaacgacaa ccccgcctcc cgcacaataa agaattcctc ctcactcgcc
     4021 agcttttcat attgatcctg ccgaccggcc ttgcgttttc gctgacgata ataaattttt
     4081 tcgtcgggcg tctccagcac ttcactgatg acgggcatac aactctccag ccactccgca
     4141 tgcacttcat cggtcatgcc atgccggcgc tgatattcgg cgagatacag tttgtcctca
     4201 taccgctcga tacaaaacgg aaattcggga agatcatgat catagattcg ataacagccg
     4261 atcccctgcc gcctcgccag ccgcccgata tgacgataat ccttcgtcag ccggttgcgg
     4321 aacatctgta acttttcatc ctgcatggac aaccggtttg atttgttgta acagcctcct
     4381 gaaccctgcc gcggccagca gacagacccc gcagaccagc caccacaacg gcctgaatcc
     4441 ccagtgctgt gctatctccg cgccgccggc aggacccacc acctgcgccg tggaccaggc
     4501 gatcgtatac agtcccgcat actgccccct gttggatgcc gcggtccgcg agatccagaa
     4561 cgaattcatg aacggcattg ccgtgatctc gcccgcggtc atcaggatca tacaaaaata
     4621 agcggtatag actgacaggg gaaagacatt aagcaacaaa taagatgctc ctacgaacaa
     4681 cacgccgcag gaaatataat acaggacttc gcgccgtcct tccaacttga acacgagcac
     4741 catttcaaaa aaggtgatca ataacccgtt cagggtcatc aacagtccga tcatatattc
     4801 cggcatcccc agcatcttct tgtaaaacac cggcaacacc gaaaacatct gaaaaaaaca
     4861 ggacccaaaa agaatggtac cggcgataaa cagcagatag atcctgtccc tgtagggcga
     4921 taacgccgga ccctcgccgt tattgatccg gtcacccggc gtcgatgccg acgacggagc
     4981 caaaaacatc cgcagcagca acgccgccag cagattcgtg accccgtcga cccagaacag
     5041 caggtgaaaa ttgatggagg ccagtattcc cccgatagcg cctccgaccg cccagccgag
     5101 attgacggcc aaacggttca gcgaaaacga gcgcgtccgg ttctcttctt tactatatgc
     5161 ggcgatagcc gtggaattgg ccggccggaa agcgtcgttg acgacactca gcagaaagga
     5221 gcagatacag atgagcggat agctcttcat ctgacccaaa aggataaaca aaaagcctcc
     5281 tcccgtcagg gtcaccagct gcacccggtg aaaaccgatc cggtcggcca tccgtccgcc
     5341 caaccagccg ccgcacatgg ccccgagacc aaagatcccg atcaccaggc ccgcctgacc
     5401 gatggtgtaa cccatcgtcg gctgcgtcag atagatcgtc atgaacggaa agaccatcgt
     5461 gccgctgcgg ttgatcaaca tgaccaacga caacaaccag gtctcccgtg agagaccgct
     5521 ataagccttt ttgtaaaggg aaaacgtccg ggtgagcata ccccgcgaag atactatgta
     5581 tatccctgcc tatcccagac cccagccatt tttccgcatc acaaacgcta tcgcgttccg
     5641 gagccaaaca ccttcttcag cagatcgtcc acccgcgccg cagggttctg ccggatattt
     5701 ttctcttcca ccgccacata aaagaaaacg ccatacaacg ccctgtccgt cgcataggaa
     5761 ggcagatcgg gattcacctt gtggaacgtc gtggggaatt tgttataagt atcgaaaata
     5821 tccttccagt atttggtcgc gccagtcttt tctaaggatg ccttgatcac cggcaggaac
     5881 gcggccgtca gctgcgcgga ggtcgatttg cgcagatacg ccgttgcagc cgaatcactg
     5941 cccctcagga tactgagtcc atccgatacg gtcatattcc tgatagcatc gagaaagatc
     6001 ggagccgcct tggtcgccgc gtcttccgcc gcccggttga tgctcagtat cgcatcatcc
     6061 acctgtttgc cgaacccggc gcttcgcagg gtcttttcta ccttgaccgc atccggagga
     6121 aggaggattt tgaccgcggc gtccttgaag aatccgtcca ccgccgacaa tttgtccgta
     6181 ctatgttgtg tgccatggtt cagggcatcc ttcagacctg caacaatatc atccgagctc
     6241 agccctttac cgccttttaa cgagttggcc gcacttttgg ccttatcgag cagactgctc
     6301 tgggcattaa cgatgaccac ggaaaataag gagaaagtta cgacagtg
//
LOCUS       DASYSV010000243         5834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1325318, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000243 DASYSV010000000
VERSION     DASYSV010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325318"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VGQ51_07495"
     CDS             complement(<1..920)
                     /locus_tag="VGQ51_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEV9036445.1"
                     /translation="MKVAVAILFLFIGSHALANPIPFPPGRALAKLIIVDATGHGDFT
                     TIQAALNSLSTDSAATRLIFIRNGIYHEKVFIEKNNIELKGEDKEKTIITFALARDAW
                     RCDHKDDWGVATLNLRGSDITLRNLTICNSWGFDNTAATTEVACAADSPTHQKVVGRQ
                     GHQMALRSFQTTRLRVIGCILKAYGGDTVSPWNVSAGFFYFRDCSMEGGVDFYCPRGW
                     AYAEHCSFHADDGPACIWHDGSADPDSKTVLKDCSFSGYDGFRLGRYHRDAQFYLIDC
                     HFAPNMADEDIYLVKTTNVIQWGRRVFYYH"
     gene            complement(923..1969)
                     /locus_tag="VGQ51_07500"
     CDS             complement(923..1969)
                     /locus_tag="VGQ51_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9036446.1"
                     /translation="MIPVRTAICSFGMSGRVFHAPFLHVHPGFKFYSVWERSKDLAEA
                     LYPGVRTCRSLEELLADPAVELVIVNTPNYTHFDYARAALEAGKHVIVEKPFTVTVQQ
                     GNELIALAKQKGKKLSSYQSRRFDSDFRTVRRVIGSGVLGTIVEAEIHYDRYTPALSP
                     KVHKETPGPGTGLLYDLGAHLIDQALQLFGDPEAVFADLFIIRDDSKVDDYMEVLLFY
                     PGLRVRLKSSYLVREPLPAYSIFGHTGTFLKTRADIQEKHLLAGEMPDSAEWGIEPES
                     EQGLLHTVIDGNTVRERVPTEKGSYMDYFNGIYESIRNDAPLPVTAEEGVRIIRVIEL
                     AYESLNKKQIVPFR"
     gene            complement(1972..3621)
                     /locus_tag="VGQ51_07505"
     CDS             complement(1972..3621)
                     /locus_tag="VGQ51_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEV9036447.1"
                     /translation="MRNLLIAAALLSCCSTVAAQDYSWDHLPVIHRPVFKHDTFSIAH
                     YGALPDGITVNTKAIDDAIADCSRKGGGVVLIPQGLWITGPITLRSGVNLHVDRAAIL
                     QFSPDKALYPIVAGNWEGHPAARCQSPISGTDLENIAITGGGIIDANGDSWRWISKDR
                     LTEGEWKQKVASGGVVTEDGRTWFPSAQSLKGYRTKEAGVLKPGMSVEDFREIKDFLR
                     PNLLVLTNCRKVLLEGATFQNSPAWCLHTLLCEDLTLEDVHVRNPWSAANGDGIDVES
                     CKGVLIERSTFDAGDDGICVKSGRDEDGRKRGRPTENMIVRDDIVYRAHGGFVIGSEM
                     SGGARNIFVYNCSFIGTDIGLRFKTVRGRGGVVEKIFIKDIAMRNIVHQAVFLDMYYF
                     AKPPAIADVYDGKAKVEIPPVTEATPRFRDIHISHIVCDGAEEGIFVRGLPEMNIRDI
                     YLDHMVLRAKKGAEIIEAQNITLKDIRLVTSDSTPVVYVENSSNLNFEDIHYPPGAGL
                     LFSINGDKCANIRVEGTDRSKAVHDAAFRYGAASSALQLKD"
     gene            complement(3628..>5834)
                     /gene="pelA"
                     /locus_tag="VGQ51_07510"
     CDS             complement(3628..>5834)
                     /gene="pelA"
                     /locus_tag="VGQ51_07510"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEV9036448.1"
                     /translation="LIDLDAESQVLLQQMGPEYSKLLFDYLSPRENPHYPEGRMDDTH
                     FNELGARRMAEIVLEDIRALKLGLAAHIFSPAVTPHTAAARIDYAPFADNSGHWYSIF
                     DKEDIIHPLPGHPRLAPTDIRDIADNILLFQKDNGGWAKNYDVFAVLTPGQKDSVAAA
                     KKETNTTFDNGSTYNQVAALATAWLASKDTRYKDAASHGLEFILKAQYKNGGWPQYYP
                     LRDDYSRCITFNDGAYEGIIRLLKDIRDGASQYAFIDAALRKRLDEAYEKGMDCILKT
                     QIADMGRPTAWCQQYDARTLQPAWARKFEPPSICNRESAGLVLFLMGIDHPDQRIQTA
                     IENAVAWFRASRIYNTRVRTIPADRMVTPFRVSLTDRVVVMDSSAPPIWTRYYELKTH
                     RPLFCNRDSKVVYSLAEVLRERRDGYGWYTYEPEQVLDRYAAWRLKQSGHPITVAADG
                     SGDYRTAQAAFDACPPHSTIYIKGGVYREKLLLTKDSITLLGDDNTILTFDDHPGKLA
                     ANGDSINTRNSYSFRVTGNNFTARNITFRNDAGFTAGQAVGVEARGDREAFIDCRIIG
                     NQDILFLNNPDSRQYYEDCTIEGTTDFIFGDATAWFEHCTIHSKKNSHVTAASTPKDH
                     AYGFVFDHCTLTGDSSLAKVSLGRPWRPYASVTYLHCWLGRHILPAGWSNWNTTDNYK
                     TARYAEFDDNGPGAETAARVPWSRQLTSSQAAEITPRTVFGDWNPMEIKTTK"
BASE COUNT         1200 a         1586 c         1694 g         1354 t
ORIGIN      
        1 caatggtaat aaaagacgcg gcgtccccat tggatcacat tggtcgtctt caccaggtaa
       61 atatcctcat cggccatatt cggggcaaaa tgacaatcga tcaggtagaa ctgcgcgtcc
      121 cgatgatagc ggcccagccg gaagccatca tagccgctga agctacagtc cttcaatact
      181 gtcttcgagt cgggatcggc cgagccgtca tgccagatgc aggccggtcc gtcatccgca
      241 tggaagctgc aatgctcggc ataagcccag ccccgggggc aataaaaatc caccccgcct
      301 tccatgctgc agtccctgaa ataaaaaaat ccggcgctga cgttccaggg gcttacggta
      361 tcgccgccat atgctttcaa tatgcagccg atgactctta gccgtgtcgt ctgaaatgat
      421 cggagggcca tctggtgtcc ctgtctgccg accacctttt gatgggtcgg cgaatcggcg
      481 gcgcaggcca cttcggtggt cgcggcagta ttgtcgaagc cccagctgtt gcagatagtc
      541 aggttgcgga gggtgatatc gctgcctcta aggttgaggg tggcgactcc ccaatcgtcc
      601 ttatgatcgc agcgccaggc atcacgcgcc agcgcaaagg taatgatcgt tttttctttg
      661 tcttctcctt tcagctcgat attgttcttt tcgataaaga ctttctcgtg atagatgccg
      721 ttgcggataa agatcaggcg ggtcgcggct gaatccgtag ataaactgtt gagtgctgcc
      781 tggatcgtcg tgaagtcgcc gtgaccggtg gcatcgacga taatgagctt ggcgagggcg
      841 cgaccgggag gaaaaggaat cgggttggcc agggcatggc tgccgatgaa aaggaataat
      901 atcgcgactg caactttcat ttttatcgga aaggaacgat ctgttttttg ttcaggctct
      961 catacgccaa ttcgatgacc ctgatgatcc tgactccttc ttccgcggtg acgggcagcg
     1021 gcgcgtcgtt gcggatggac tcatagatac cgttgaaata gtccatatag ctgccttttt
     1081 cggtgggcac ccgttcgcgg accgtgtttc catctatgac cgtgtgcagc aaaccctgct
     1141 cgctttccgg ctcgattccc cattcggcgc tgtccggcat ctctccggcc agcaaatgtt
     1201 tttcctgtat gtcggcgcgt gtttttaaaa aagtccccgt atgaccgaaa atgctgtaag
     1261 cgggaagtgg ttcccgtacg agatagctcg atttgagccg gacccgaagt cccggataga
     1321 acaacaggac ttccatatag tcatcgactt tggagtcgtc acggatgatg aacagatctg
     1381 cgaacactgc ttcggggtct ccgaaaagct gtaaggcctg atcgatcagg tgggcgccca
     1441 gatcatagag gagacccgta cccggtcccg gagtctcctt gtgtactttg ggacttagag
     1501 cgggcgtata ccggtcgtag tggatctccg cctcgacaat ggtgccgagt acacccgatc
     1561 ctatgactcg tctcaccgtc cggaaatcgc tgtcgaaccg gcggctttga tacgaagaaa
     1621 gctttttccc tttctgcttc gcaagcgcga tcagctcatt tccctgctgg acggtgacgg
     1681 tgaagggttt ttcgacgatg acatgctttc cggcttccag cgctgccctg gcatagtcga
     1741 aatgtgtgta gttcggcgta ttgacgatca ccagctcgac cgcggggtca gccagcaatt
     1801 cctccaggct cctgcaggtg cggacgcccg gatatagagc ctcggcaaga tccttgctcc
     1861 tctcccatac cgaatagaac ttgaaacccg ggtgcacatg gaggaacgga gcgtgaaaaa
     1921 cacgcccgga cattccgaag gaacaaatgg ccgtcctgac aggtatcatg ttcaatcttt
     1981 taattgcaat gccgaagatg cggccccata tcggaaggcc gcgtcatgga cggctttcga
     2041 acgatcggta ccttccacgc ggatattcgc gcatttatct ccgttgatgc tgaaaagaag
     2101 accggcaccc gggggataat ggatgtcttc gaaattcaga ttgctgctgt tctcgacata
     2161 cacgaccggt gtcgaatcgc tggtgaccag ccggatatcc ttcagcgtga tattctgcgc
     2221 ttcgatgatc tccgcgcctt ttttagccct cagcaccata tgatcgagat aaatatcccg
     2281 gatgttcatc tctggcagtc cacgtacaaa tataccttct tcggcgccat cgcaaacaat
     2341 atgactgata tgaatatcac ggaatctcgg tgtcgcctcc gtgacgggtg gtatctccac
     2401 tttggcctta ccgtcataca catcggcgat ggccggcggc tttgcaaaat aatacatgtc
     2461 caggaaaacg gcctgatgga cgatattgcg catggctatg tcctttatga agatcttttc
     2521 tacgacgccg ccacggccgc gaaccgtctt gaaccgaaga ccgatatcgg tgccgatgaa
     2581 gctgcaatta tagacaaaga tatttcgggc cccgccggac atttcgctgc cgatgacgaa
     2641 gccgccatga gcgcgataaa cgatatcgtc ccggacgatc atattttcgg tcggcctgcc
     2701 tcgtttgcgt ccatcctcat cacgtcccga cttgacgcag atgccgtcat cacccgcgtc
     2761 gaaggtgcta cgctcgatca acaccccttt gcaggattcc acgtcgatcc cgtcgccatt
     2821 ggcggcgctc caggggttac gcacatgtac gtcctccagc gtcagatcct cacacaataa
     2881 ggtatgcaaa caccaggctg gtgagttctg gaaggtagcg ccttcgagca ggacttttct
     2941 gcaattggtg aggaccagta aattgggtcg aaggaagtcc ttgatctccc tgaagtcctc
     3001 caccgacatc cccggcttca gcacgccggc ctctttggtc cgatagcctt tcagggactg
     3061 ggccgaaggg aaccaggtcc tgccgtcctc agtgacgacg ccgccggaag ccactttctg
     3121 tttccattca ccctcggtga gccggtcttt ggatatccag cgccaggaat ctccattggc
     3181 gtcgatgatc cccccgcctg tgatggcgat attctcgaga tcggtaccag agatggggga
     3241 ctgacaacgc gcggcaggat gtccttccca attccccgcg actatcggat acagggcttt
     3301 gtcgggactg aactgcagga tggcggcgcg gtcgacatgc agattgacgc cgctgcgcag
     3361 ggtgatcgga ccggtgatcc agagtccctg gggtatcagc actaccccac cccctttgcg
     3421 gctgcagtcc gcgattgcat catcgatagc cttcgtattg acggtgatac cgtcgggcaa
     3481 cgcgccgtaa tgtgcgatcg aaaaggtgtc gtgtttgaag acgggtcggt gaatgaccgg
     3541 gagatgatcc caggaatagt cctgtgcagc aaccgtgctg cagcagctaa gaagggcggc
     3601 ggctataagt aagtttcgca tagacttcta tttagtcgtt ttgatttcca tcgggttcca
     3661 atcgccgaag accgtgcgcg gagtgatctc cgccgcctgt gaagacgtca gctggcgcga
     3721 ccagggtacg cgtgcggcgg tctccgcgcc ggggccgtta tcgtcaaatt cggcataacg
     3781 ggcggtctta taattatccg tggtattcca attggaccat ccggccggca ggatatgccg
     3841 tcccagccag caatgaagat aggtcacgct ggcataagga cgccagggtc tgcccaaaga
     3901 aacttttgcg agcgacgaat cgccggtgag cgtacagtga tcgaatacga aaccataggc
     3961 atggtccttc ggcgttgagg ccgcggttac atgcgagttc ttcttgctgt ggatggtgca
     4021 atgctcgaac caggccgtgg cgtcgccgaa aatgaaatcg gtggtaccct cgatcgtaca
     4081 atcttcgtaa tactgccggc tgtccgggtt gttcaggaaa aggatatcct ggttgccgat
     4141 gatccggcaa tcgatgaatg cttccctgtc gccgcgggct tccacgccca cggcctgccc
     4201 ggccgtaaaa ccggcatcgt tgcggaaggt gatattgcgg gcggtgaagt tattgcccgt
     4261 cacgcgaaag ctgtaggagt tgcgggtatt gatgctgtcg ccattggctg ccagcttgcc
     4321 gggatgatcg tcgaaggtga gtatggtgtt atcgtcgccg aggagcgtga tgctatcttt
     4381 cgtgaggagt aatttttccc ggtagactcc gcctttgata tagatggtcg aatgaggcgg
     4441 acaggcgtcg aaggccgcct gcgcggtgcg gtagtcgccg gagccatcgg cagctacggt
     4501 aatgggatgc ccgctttgtt tgagacgcca ggccgcatag cggtcgagca cctgttcggg
     4561 ttcgtaggtg taccagccat agccatcgcg acgttcgcga aggacttcgg cgagcgagta
     4621 aacgactttg ctgtcgcggt tgcaaaagag ggggcgatgg gtctttagct catagtaacg
     4681 cgtccagatg gggggcgcgg aggaatccat caccactacc ctgtcggtca gcgagacgcg
     4741 gaagggcgtg accatcctgt cggcgggaat ggtcctgaca cgggtgttgt aaatacgtga
     4801 tgccctgaac caggccaccg cattttcgat ggctgtctgt attcgctgat cgggatgatc
     4861 gatgcccatc aggaacagga cgagacccgc gctttcccgg ttgcagatgg aaggcggttc
     4921 gaatttgcgg gcccaggcgg gttgcagtgt ccgggcgtcg tattgctgac accaggcggt
     4981 gggccggccc atatcggcga tctgggtttt gaggatgcag tccatcccct tttcgtaggc
     5041 ttcatccagc cgtttgcgga gcgccgcgtc gatgaaggca tattgagagg cgccgtcccg
     5101 gatgtctttg agaagacgta tgatcccctc ataggcgccg tcgttgaagg tgatgcagcg
     5161 gctataatcg tcgcgcaacg gataatactg cggccagccg ccattcttat attgcgcctt
     5221 caggatgaat tcgagaccgt gcgatgccgc gtctttgtat cgggtgtcct tcgacgccag
     5281 ccaggctgtt gccagcgcgg ctacctggtt ataggtgctc ccgttgtcga aggtggtatt
     5341 cgtctctttt ttggcggcgg cgacgctgtc cttctgtccg ggagtgagca ccgcgaacac
     5401 gtcgtagttc ttggcccatc cgccattgtc cttttggaaa agcaggatat tatccgcgat
     5461 gtcgcgtatg tcggtgggcg ccagacgagg gtgacccggc agcgggtgga tgatgtcctc
     5521 tttgtcgaat atgctatacc agtgaccgga attgtcggcg aagggagcgt agtcgatcct
     5581 tgcggccgca gtatgcggtg tgactgccgg cgaaaagata tgcgccgcca gaccgagttt
     5641 cagcgcccgg atatcttcca gcacgatctc cgccatacgg cgcgcgccca gctcattgaa
     5701 atgggtgtcg tccatccgac cctccggata atgagggttc tctcgcggcg acaggtaatc
     5761 gaacagcaat ttggaatatt cgggccccat ctgctggaga aggacctggc tttcggcgtc
     5821 gaggtcgatc agcg
//
LOCUS       DASYSV010000244        10370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1706709, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000244 DASYSV010000000
VERSION     DASYSV010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706709"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VGQ51_07515"
     CDS             complement(<1..972)
                     /locus_tag="VGQ51_07515"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9036449.1"
                     /translation="MAEASIHTTPATESTFISADIRNSGLLRRRWLYHLAFWVPYFGL
                     LVIFYLSLHMTMTTSLFTIVFFTVVIEAGFSYFNLYVLITRLLFARRYLFYILAFLLT
                     VMACSVAVLLMETVVVRWIAHEPAAAYALWMPKNIIVGTVMGVYVLGLTTGIKFAKDA
                     LLDKELRKEREKHYLETELKFLKSQIQPHFFFNTLNNIYSLTLNRSEQAPEVILKLSD
                     IMSYILYDSTAPLVPLAKEIDYLRNYVDVEQLRFGNRLSVSFHIDGPVDAAEIPPMIL
                     ILFLENSFKHGVKNNISRIHLAIDLRLADGYLHFQVENPAPEEETTGE"
     gene            complement(1063..3516)
                     /locus_tag="VGQ51_07520"
     CDS             complement(1063..3516)
                     /locus_tag="VGQ51_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV9036450.1"
                     /translation="MRRLTLLVVAAAVCGAVLAQDNTGYQLPPKDLADLLLAPPTPLV
                     SVDRKGQWMLLSRRPSYPSVEELAQPELRIAGLRFNPGNYALSRQNFTNGFSLKNLGT
                     GQEYPIQGLPVPLMAGNIAWSPGESKIAFTNTTGHRVDLYVIDIATRKAARVNATALN
                     TITGGAYRWIDDKTLLYKIILKPANAAPPRQLVPKGPAIQETLGKSAPSATFEDLIRS
                     PYDESLFAFYGEARLVKNIGGVETPIGKPGIFVSIEVSPDRRFILLRTIHKPFSYLVP
                     ANRFNATVAVIDVTGRPIKTIADLPSGESTPRGFDNTQNQPRDFGWRADKPSTLFWCT
                     PLDSGLMRKRMEFHDAVYELPAPFTGDAKPLFRTKLRFADIIWGNDSTALVFELLRSR
                     QMEHLLRFNPATGETSLLIQRNTTDAYSDPGAPVLIRNRFGEPVLRTVDNGTRLLLNN
                     PVGSSPDGDRPFLSKFDLGSGHNDIVWRCPSGRFEFISEVLDADRLILLSREESPREV
                     PNYYIKDLSSHTADKPVTSFVNPYPQLEGITREKVHYKRADGVDLTGDLYLPKGYDAG
                     RDGPLPVLLWAYPREFNSAADAAQVRGSQFRFTTINWASPVFWVIRGYAILDNAEMPI
                     VATDSSKKPNDDFVDQLRLNAEAAIHKLSAMGVGDSNRVAVGGHSYGAFMTVNLLAHT
                     KLFKAGIARSGAYNRSLTPFGFQNEDRTYWEDPKLYYDMSPFSYADKITTPLLLVHGE
                     MDDNPGTFPIQSERLYNAIKGHGGTVRYIVLPYEAHGYRGRENLLHLLYEENAWLDKY
                     VKNAPRQPAGADTHRQKAF"
     gene            complement(3608..4450)
                     /locus_tag="VGQ51_07525"
     CDS             complement(3608..4450)
                     /locus_tag="VGQ51_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036451.1"
                     /translation="MSAPDAPKARSGNFLLVGITLTISILLEQYWIARLFPQLGQVGG
                     LRPMIVFLDIPLALPKIDWIPVGFLFIFFYGMVISSELNHRGQAPGALRRKKIAAALT
                     GWWLLLICILAGGGLYSLIQGFLPRGVRNGIDSFGVRADITLPYPSGELVHLHGSMIL
                     LVFTGFGIRLLLKKTALPPVVVPVAVAAPAPPRKRSPARVATPTGKKVPAPAPKSQPA
                     TAAPTYRRIPDTVHDQYITPEPAPDCRLTTPPPVAVILPKPGPRIGSVHPCIVAGMLT
                     PAKT"
     gene            4560..6041
                     /locus_tag="VGQ51_07530"
     CDS             4560..6041
                     /locus_tag="VGQ51_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036452.1"
                     /translation="MQRIAKEALKRAGYRAWLFPVLAAEWILFKCCYPYADYFTDSYS
                     YIGAAAAHDAIGYRPIGYSIFLRAVHWIWASDTFLVTLQYVLVQTASLGLILWLRRWC
                     SLSERTLRVLFAFLILNPLIPYTCNYVSSDALFIALSLVWLIVLLELLRRPSWRWLIV
                     QIILLFVIFHLRYLAMYYPAVSALTVLLAKKAKPVLKLAGVAGSIGVIAFSISLVRQT
                     TFRETGAPVFSAFSGWQIASNAMNMYPYIHTDTTGLPSPECLRLEEDVRAYFDKMGPS
                     LRDRGPSATTEYMWVGNSPLHVYLAEYRRHTATDYFTAWNRVAPVFTQYGYFLIRRHP
                     WAYVRYYMWPSAVGFFWSPLDVFAVYNEGHETVDPVARDWFHYKTLRPQVLSATVQGR
                     LLAPMPAVYLALNIVFIVIVVVFLSSRVRRDLHPLFSGCLRLGSAYLLANASFCIVAS
                     PSVFRYQVLPVIILFVLSTCGFYCLTFQYGQIGKPSPASDRRD"
     gene            5995..7260
                     /locus_tag="VGQ51_07535"
     CDS             5995..7260
                     /locus_tag="VGQ51_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEV9036453.1"
                     /translation="MVKSASLHLPVIAGIKEYRRQAEELFAAYMAGDAGVRALIDEHH
                     PRLRRLAAGEDNPMPVTLADIQLSMADWYYHESWPHLEEWADEASSPGSRVSSFEAAA
                     EALLSGNAATLSALLRLYPSLVRLRSMRRHHATLLHYIAANGVEYYREEYPKNAVALL
                     DLLLAAGAEVDSKADMYGGGVTTLGLVATSIHPARAGELIPLLDRLIVAGASLDMPDA
                     AGNQQYIVNGCLANGRPEAAAYLSRRGAYLDLEGAAGVGRLDVVETFFDEDGGLKKSA
                     TRNQLGEALNWASEYGHSTVVEYLMDHGADPGETINGMQALHMALLGGHLETIRLLIK
                     RGAPLESRNQYGGTPLGMALWAIRHRDPVHTWPEKKIDNVGVIEALLTGGAEVGPGIL
                     ATLQWQPRSRIKTEIEQLLQRFGHEPWKS"
     gene            7380..8270
                     /locus_tag="VGQ51_07540"
     CDS             7380..8270
                     /locus_tag="VGQ51_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0050113 - inositol oxygenase activity
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol oxygenase family protein"
                     /protein_id="HEV9036454.1"
                     /translation="MNQSSKPLSDAETNPLPSLDVWEDDLLERYPEPGTIAKGKTTEE
                     YRNYESPGRDSVREFYRLNHTYQTYAFVQEKKAEFLSFGRKEMSVWDAFEFLNQLVDD
                     SDPDTDLDQFQHLLQTSEAIRADGHPDWMVLAGLFHDMGKVLCLFGEPQWAVVGDTFP
                     VGCAWSDKIVYPEFFAANPDVSDPRYQTRYGVYSHGCGLRNVDLSWGHDEYVYHMLKD
                     YLPEPALYMLRYHSFYSQHREEAYDHLLDDHDREMFKWVRLFNPYDLYSKNPVPPDWN
                     ALRPYYEDLVARYLPEKIKF"
     gene            complement(8279..8467)
                     /locus_tag="VGQ51_07545"
     CDS             complement(8279..8467)
                     /locus_tag="VGQ51_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036455.1"
                     /translation="MHRARHGEPPFDSVRGVFVEGRELYPGAGFQDKNHIQICIRNPN
                     CIRSFFLPRRETRWPTAA"
     gene            complement(8604..8786)
                     /locus_tag="VGQ51_07550"
     CDS             complement(8604..8786)
                     /locus_tag="VGQ51_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036456.1"
                     /translation="MTEQEYNESMETLRESRRKALRSKAAARKFLKDAGVLHLLVPID
                     KPGKKKKTSTRLPKSR"
     gene            8854..10299
                     /locus_tag="VGQ51_07555"
     CDS             8854..10299
                     /locus_tag="VGQ51_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV9036457.1"
                     /translation="MKKTLLLALAIAAGVQCWAQDYKPTWESLDKRPVPQWYKDAKFG
                     IFIHWGVYSVPGYSKVGQYAEWYQNGLRNNDTARIRYQKEVFGDLTYYQLADRFKAEL
                     YNPDEWAHVIEQSGAKYVVLTSKHHDGWANWPSAATSLAWNFPWNAGVAGPHRDLLGD
                     LFRALRKTSVHPGMYYSLYEWYNPLWLNDRSRYVREYMWPQMKDLVNTYRPDVFWTDG
                     DWEDSDTTFRSREFLAWLYNESPVKDRVVTYDRWGRGIRFHHGGVYTPEYQPDLDFKD
                     HYWEESRGMGYSYGYNRAEDSWDYNSARSLVLQLIDKVSRGGNFLLDIGPDAHGKIPP
                     IMEERLLQIGHWMKINNEAIYGAVRWKTASQWGPGRTDYKPPKGGGGDLLLKLTIDPD
                     PGFAVKESFFTYNPAQKALYVLLPKWPGGSAPFVVKDLVLPAGAQVSLLETKQNLKWT
                     QQGRDLKITMPAFDPNRMTSEYAYVIKITSI"
BASE COUNT         2219 a         2702 c         3145 g         2304 t
ORIGIN      
        1 ttcgccggtg gtctcttctt ccggggcagg attctccacc tggaaatgca gatacccgtc
       61 ggccaggcgc agatcgatgg ccagatggat gcggctgata ttgttcttga caccgtgttt
      121 gaaactgttc tcgaggaaga ggatcaggat catcggtgga atttcggcgg catcgacggg
      181 accatcgatg tggaacgaca ccgagaggcg gttgccgaag cgcagctgct cgacgtctac
      241 atagttccgc agatagtcga tctcttttgc cagaggtacg agaggggcgg tggagtcata
      301 gagaatatag ctcatgatat ccgagagctt gagaatgact tccggagcct gctcggatcg
      361 attgagggtc agcgaataga tattgttgag cgtgttgaag aagaaatggg gttggatctg
      421 ggatttgagg aatttcagct cggtctcgag atagtgtttc tcgcgctctt ttcgaagttc
      481 cttatcgagg agggcgtctt tggcgaattt tatacctgtg gtcagcccga gcacatagac
      541 gcccatcacg gtcccgacta tgatgttctt gggcatccat agtgcgtaag ccgcggcagg
      601 ttcatgggcg atccatcgga caacgaccgt ttccatgagg agaaccgcca ccgagcaggc
      661 catcacagtc agcagaaagg ccagtatgta gaataaatat ctccgggcaa aaagcaggcg
      721 ggtaatgagc acgtacagat tgaaatagga aaagccagcc tcgatcacga ccgtgaaaaa
      781 aacgatggta aagagcgagg tggtcatcgt catgtggaga ctgagataga aaatgaccaa
      841 caacccgaaa tagggcaccc agaaagccag gtgataaagc cagcgccgtc gcagcagacc
      901 ggaattgcgg atgtctgcgg agatgaaggt gctctccgtc gctggtgtcg tatggatgga
      961 cgcttctgcc aattgacgct aaaggtaaat ttttttccat ccggcgccgt gattcttcgc
     1021 tgttcggcag cgtttgtgcg atgaacagcc cgatttgacg gcctagaagg ctttctgccg
     1081 gtgggtgtcg gcgccggccg gctgccgggg tgcattcttc acatatttgt cgagccacgc
     1141 gttttcctcg tacaaaagat gcaggaggtt ttcccggccg cgatatccgt gtgcctcata
     1201 ggggaggacg atataacgga ccgtaccgcc atgtcctttt atcgcattgt agagccgttc
     1261 gctttggata gggaaggtcc ccgggttgtc gtccatttcg ccatgcacga gcagtagcgg
     1321 ggtagtgatc ttatcggcgt agctgaaagg gctcatgtca taatacaatt tggggtcttc
     1381 ccagtaagtg cggtcttcgt tctggaagcc gaacggcgtc aggctccggt tgtaagcgcc
     1441 actgcgcgcg atgcccgctt tgaacagttt ggtatgcgcc agcaggttga ccgtcatgaa
     1501 ggccccgtag ctgtgaccgc ctacggccac gcggttgcta tccccgacgc ccattgccga
     1561 gagtttatgg atggcggctt cggcattcag tcgcaactgg tcgacgaaat cgtcattggg
     1621 ctttttggag ctgtcggtcg cgacgatggg catctcggca ttgtcgagta tggcatagcc
     1681 gcggatcacc cagaatacgg gggaggccca gttgatggtc gtgaaacgga actggctacc
     1741 gcgcacctgc gccgcatcgg cggcggaatt gaattcgcgg ggataggccc atagcagcac
     1801 cggcaggggg ccgtcgcgtc ccgcgtcgta acctttcgga agataaaggt cgccggtgag
     1861 gtcgaccccg tcggcgcgtt tgtagtggac cttttcacgc gtgataccct ccagctgcgg
     1921 gtagggattg acgaaagacg tgacgggttt gtcggcggta tgggaagaca ggtctttgat
     1981 ataataattc ggcacctcgc gtgggctctc ttccctgctg agcaggatga gcctgtcggc
     2041 gtccagcacc tcgctgatga attcaaaacg gcccgaagga caccgccaga cgatgtcgtt
     2101 atgtcctgag cccagatcga atttcgacag gaagggccgg tcgccgtccg gcgagctgcc
     2161 gacaggattg ttgagcagga gccgggtccc gttgtcgacc gtgcgcagta cgggttctcc
     2221 gaacctattc cggatgagga ccggcgcgcc gggatcactg taggcatcgg tcgtattgcg
     2281 ttggatcaga aggcttgtct cgccggtcgc ggggttgaag cggagcaggt gttccatttg
     2341 gcggcttcgc aacagctcga atacgagagc cgtgctgtca ttaccccaga tgatgtcggc
     2401 aaaacggagc tttgtccgga acaggggttt ggcgtccccg gtgaagggag cagggagctc
     2461 gtacacggcg tcatggaatt ccatcctttt gcgcatcaga ccgctgtcga gaggtgtgca
     2521 ccagaagaga gtggagggct tatcggcgcg ccatccgaag tcgcggggtt ggttctgggt
     2581 attgtcgaaa ccacggggcg ttgattcacc cgaaggcaga tcggcgattg ttttgatagg
     2641 tcggccggtg acgtcgatga ccgcgaccgt agcattgaat cggttggccg ggaccagata
     2701 ggagaagggt ttatggatcg tgcgtaacag gataaatcgt cggtcggggg agacttctat
     2761 cgagacgaag atgccgggtt tgccgatggg cgtctcgacc ccgccgatat ttttcaccag
     2821 ccgggcctca ccgtaaaagg cgaatagcga ctcatcataa ggactgcgga tcaggtcctc
     2881 gaaggtggcg ctcggcgccg atttgcccag ggtctcctgg atcgcggggc cttttggaac
     2941 gagctgtctt ggcggcgcgg cattcgccgg cttgaggatg atcttgtaga gaagcgtttt
     3001 gtcgtcgatc caccggtagg ctccgcctgt gatggtattg agcgccgtcg catttaccct
     3061 ggccgccttg cgtgtggcga tgtcgatgac atagagatcg acgcgatgac cggtggtatt
     3121 ggtgaaggcg atcttgctct cgccggggct ccaggcgatg ttacccgcca tcagcgggac
     3181 gggaagaccc tgtatcgggt attcctgccc ggtaccgaga tttttgagcg agaagccatt
     3241 cgtgaagttc tgtctgctca gcgcatagtt gccggggttg aaacgaagcc ctgcgatgcg
     3301 aagctcgggt tgcgccagct cttcgacgga aggatatgag gggcgacggc tgagcagcat
     3361 ccactgtcct ttgcggtcca cgctgacgag tggagtgggt ggcgcgagca gcaggtcggc
     3421 cagatctttg ggcggcaatt gatagccggt attgtcctga gccaggaccg cgccgcacac
     3481 ggcggccgca accacgagca gcgttagtct tctcattgga gtaaggtaga aaaaatgtgt
     3541 aacttggctg acccgccaca acccgccaat taccgttcat ataaaatcgt tggttacgca
     3601 ggggacctca ggtctttgcg ggtgtgagca tgcctgcgac gatacagggg tggacgctgc
     3661 cgatgcgcgg gccgggtttc ggcaggataa cggctaccgg gggaggtgtc gtcaggcgac
     3721 aatcgggggc cggctcgggg gtaatatatt gatcgtggac cgtgtccggt atccggcgat
     3781 aggtcggagc agcggtggca ggctgggact tgggtgcagg agccgggact tttttgcccg
     3841 tcggcgttgc gaccctggca ggcgatcttt tccgcggcgg cgcaggcgcc gcgacagcga
     3901 caggcaccac caccggcggc agcgcagtct tcttcagcag gagacgaatg ccgaagccgg
     3961 taaataccaa caggatcatg cttccatgca ggtgcaccag ctcgccggaa ggatagggga
     4021 gggtgatatc ggcgcggacc ccgaatgaat cgatgccatt gcgtacccct ctcggtagaa
     4081 agccctgaat gagagagtaa agaccgccac cggcgaggat acatatcagc agcagccacc
     4141 atcccgttag tgctgccgct atcttttttc ttcgcagcgc gccgggagcc tgaccccggt
     4201 ggttcagctc ggaagaaatg accataccgt aaaagaaaat gaaaaggaat ccgaccggta
     4261 tccagtcgat tttggggagg gcgagcggga tgtccaggaa aacgatcatc gggcggagac
     4321 cacccacctg acccagttgg gggaagagac gggcgatcca atattgctcg aggagaatgg
     4381 agatcgtgag ggtaattccc acgagaagaa aattcccact ccgggccttt ggggcgtcag
     4441 gggctgacat aaacttattt tttactttga tggttggctg ttaaagtccg ggtagaaaaa
     4501 tattcatgcc gatgcgggga aattattgta attagaacgg agaattatca gcaagtaata
     4561 tgcagcgtat agccaaagaa gcgttaaaac gcgccgggta ccgggcctgg cttttccctg
     4621 tcctggccgc ggagtggata ctgttcaaat gttgctatcc ctatgccgac tatttcaccg
     4681 atagctattc gtatatcggg gcagccgcgg cacatgatgc tatcggttac cgtcccatcg
     4741 ggtattcgat ctttctccgg gcggttcatt ggatatgggc ttcagacact ttcctggtga
     4801 ccctccaata tgtattggtg cagacagcct cattgggact gatcctttgg ctgcggcgat
     4861 ggtgcagtct gagcgaacgt accctgaggg tcctgtttgc ttttcttatc ctgaacccgc
     4921 tgatccccta cacctgcaac tacgtttcga gcgacgccct gttcatcgcc ctgagcctgg
     4981 tctggctgat agtgctgctg gaattgttga gaagaccgtc atggcggtgg ctgatcgtgc
     5041 agatcatcct gctgttcgtg atctttcatc tccggtacct ggccatgtat tatcccgccg
     5101 tgtcggcatt gaccgtgctg ctggcgaaaa aggcgaagcc tgtcctgaaa ctggcgggtg
     5161 tggccggcag catcggcgtc attgcgttta gcatttcgct tgtgcggcaa acgacctttc
     5221 gtgagacagg tgcgccggta ttttcggcat tcagcggttg gcagatcgcc agcaacgcaa
     5281 tgaatatgta cccttatatt cataccgata ccaccggtct tccctcgccg gaatgtcttc
     5341 ggctggagga ggacgtccgg gcgtatttcg acaagatggg accctcgctg cgggaccggg
     5401 gaccttcggc aaccaccgaa tacatgtggg tagggaactc gccgcttcat gtttatcttg
     5461 ccgaatatcg ccggcacact gcaacggatt attttacggc ctggaatcgg gtggcgccgg
     5521 tatttacgca atacggctac ttcctgatcc gccggcatcc atgggcgtat gtccgctatt
     5581 atatgtggcc aagcgcggtg ggtttcttct ggtcgccgct ggatgttttc gccgtttata
     5641 atgagggtca cgagacagtc gacccggtgg cccgggactg gtttcattat aagaccttgc
     5701 gaccgcaggt attgtccgct acggttcagg gcaggctttt agcaccgatg cctgcagtct
     5761 acctggcact gaacatcgtc tttatagtga tcgtggtggt attcctatcc tcccgcgttc
     5821 gccgggacct gcacccgtta ttttccggct gtctgcgttt ggggtcggca tatctgctcg
     5881 ctaatgccag tttttgcatc gtggcttcac ccagtgtttt tcgctatcag gtgttgccgg
     5941 ttatcattct tttcgtattg agcacttgtg gattttattg cttaactttt cagtatggtc
     6001 aaatcggcaa gccttcacct gccagtgatc gccgggatta aagaataccg gcgccaggcg
     6061 gaagagcttt ttgcggcgta tatggcaggc gatgccggag taagggccct gatcgatgaa
     6121 caccatcccc gattgcggcg tctggcggct ggagaagaca acccaatgcc cgtcacgctc
     6181 gccgatattc aactctccat ggcggattgg tattaccacg aaagctggcc gcatctcgaa
     6241 gaatgggcgg atgaagcttc ctcgccgggg agccgggtgt cttccttcga ggccgcggcg
     6301 gaagcccttc tttccggcaa tgctgccacg ctgagcgccc tccttcggct ctatccttcg
     6361 ctggtgcggc tccgaagcat gcgcagacat cacgccaccc tgttgcatta tatcgccgcc
     6421 aacggcgtcg aatattaccg ggaggaatac ccgaagaacg cggtggcctt gctggacctg
     6481 ttgctggcgg ccggggccga agtcgactcg aaggcggata tgtatggtgg cggagtgacg
     6541 acgctcggat tggtggcgac gagtatccac ccggccaggg cgggggagct cattcccctg
     6601 ctggacaggc tcatcgtcgc cggcgcctct ctcgacatgc ccgacgcggc cggcaatcaa
     6661 caatacatcg tcaatggttg cctggcgaac ggccggcccg aagcagcggc ctatctgtcg
     6721 agaaggggag cgtatctcga tctggaagga gcggccggcg tgggcaggct cgacgtggtg
     6781 gaaacatttt tcgacgagga cggggggttg aagaagagtg cgacaagaaa tcagctgggc
     6841 gaggccctga attgggcgag cgaatatggt cattctaccg tggtggaata cctgatggat
     6901 cacggcgccg atccggggga aaccatcaac gggatgcagg ccctgcatat ggcattgctg
     6961 ggtggccacc tggaaacgat aaggctgttg atcaaaaggg gcgcaccgct cgaatcgcgt
     7021 aaccaatacg gaggcacccc gctgggaatg gcgctatggg ctatccgtca tcgcgatccc
     7081 gtgcatacct ggcccgaaaa aaagatcgat aacgtcggcg tgatcgaagc gttgctgacc
     7141 ggtggcgccg aagtaggacc cggtatcctt gccacgctgc agtggcaacc ccgcagcagg
     7201 ataaagacgg agatcgagca actgctgcag cgtttcggcc atgaaccctg gaagtcgtaa
     7261 ataggggggt gtcgccacaa cggactgccg cccccaccga tctgccgcaa cggactgccg
     7321 cccccaccga tttgccacaa cggacaattt tcattaactt atggcatcaa actgacctca
     7381 tgaatcaatc atccaaaccg ctgagcgacg ccgaaacgaa tcctttgcct tccctcgacg
     7441 tatgggaaga tgatctcctc gagcgttatc ccgaaccggg gacgatcgcc aaaggcaaga
     7501 cgacggagga ataccgcaac tacgaatcgc cgggccgtga ttccgtccgc gagttctacc
     7561 ggctgaatca tacctaccag acctatgcgt tcgtgcagga aaagaaagcc gaattcctgt
     7621 cattcggcag aaaagagatg tcggtatggg atgccttcga attcctcaat cagctggtgg
     7681 atgacagcga ccccgatacc gatctcgatc agttccaaca tttgttacaa acttccgaag
     7741 ccatacgtgc ggacggccat cccgactgga tggtgctggc gggtcttttt cacgacatgg
     7801 gcaaggtgct ctgtcttttc ggcgagcccc aatgggccgt cgtcggcgat accttccccg
     7861 tgggttgcgc gtggagcgac aagatcgtgt atcccgaatt ttttgcggct aaccccgatg
     7921 taagcgatcc ccggtaccag accagatacg gcgtttactc gcacggctgc ggtctgcgca
     7981 atgtcgatct ttcctggggt catgacgaat acgtgtatca catgctgaaa gattatctgc
     8041 cggagccggc cttatatatg ctgcgttatc attccttcta ttcgcagcac cgtgaagagg
     8101 cctatgacca cctgctcgat gaccatgaca gggaaatgtt caaatgggtc aggttgttca
     8161 atccctacga tctttattcg aagaacccgg tgccgccgga ctggaatgcg ctccggccat
     8221 attatgagga cctcgtggcg aggtatctgc cggagaagat aaaattctaa gatttgcttt
     8281 aggcggccgt gggccagcgg gtctccctgc gggggaggaa gaagcttctt atgcaattag
     8341 gattgcggat gcatatctgg atgtggtttt tgtcctgaaa gccggcgccg ggatagagtt
     8401 cgcggccttc gacgaagaca ccgcggacgg agtcgaaggg aggttcgcca tgtcgggcac
     8461 ggtgcatatg caagtattca ccgacgctgc aatccagttc ccttatcagc aggtcgttag
     8521 agtctcttgt tggccgatcg acaggcaatt tctggcaacc ctggtcttat gggatacata
     8581 tcaaataatt aatcaaatgg tacttagcgg gacttcggaa ggcgtgtgct cgtttttttc
     8641 tttttgccgg gtttgtcaat cggaacaaga agatgaagaa ctccggcatc cttaagaaat
     8701 tttcttgcag cggccttgga acgcagcgcc ttcctccgtg actcgcgcaa tgtttccata
     8761 gattcattgt actcttgttc tgtcatacca taaatttaaa atactttccc cactacttaa
     8821 aaagaacttt tatcttcgaa aaaaaactgc cgaatgaaaa agaccttatt gctggcgctc
     8881 gcaattgccg ccggagtgca gtgctgggcg caggactaca agcccacctg ggaatccctg
     8941 gacaaaaggc cggtgccgca atggtacaaa gacgccaaat tcgggatctt tattcattgg
     9001 ggcgtttatt cggtgccggg atacagtaag gtggggcagt atgcggaatg gtaccagaac
     9061 ggtctgcgca acaacgatac ggcgcgcatc cggtatcaga aggaagtatt cggcgatctg
     9121 acgtattacc agctggcgga ccggttcaag gccgagctgt ataaccccga cgaatgggcg
     9181 catgtaatcg aacagtcggg agcgaaatac gttgttctca cctcgaagca tcatgacgga
     9241 tgggccaact ggccgagcgc cgctacctcg ctggcatgga acttcccctg gaatgcggga
     9301 gtggcggggc cgcatcgcga tctgctgggc gatctgttcc gcgcgctgcg caagacttcg
     9361 gtgcacccgg ggatgtacta ttcgctatat gagtggtata atccgctgtg gctgaatgac
     9421 aggtcgcgct atgtccggga gtatatgtgg ccgcagatga aggacctggt caatacctac
     9481 cggcccgacg tcttctggac cgacggagat tgggaggaca gcgacactac cttccgcagc
     9541 cgcgagttcc tggcatggct gtacaatgaa agccccgtga aggaccgcgt cgtcacctat
     9601 gaccgctggg gcaggggaat ccggttccat cacggcgggg tatatacgcc ggagtaccag
     9661 cccgatctgg atttcaagga tcattattgg gaagaaagcc gcggcatggg ctattcgtac
     9721 ggctacaacc gcgccgaaga ttcatgggat tataattccg cccgctcact ggtgctgcaa
     9781 ctgatcgata aggtaagccg cggagggaat ttcctgctcg atatcggtcc cgacgcgcac
     9841 ggcaagatac cgcccattat ggaagaacgg ttgctgcaga tagggcactg gatgaagatc
     9901 aacaacgaag ccatctatgg cgccgtccgg tggaaaaccg cttcgcaatg gggacccggg
     9961 cgcaccgatt acaaaccgcc aaaaggcggc ggaggcgatc ttctgctgaa gctgaccatc
    10021 gatcccgatc ccggctttgc cgtaaaggaa tcttttttca cctataaccc tgcacagaag
    10081 gcgttatatg tgctcttgcc gaaatggccg ggaggcagcg ctccgttcgt cgtgaaagac
    10141 ctcgtgttgc cggccggtgc acaggtcagc ctgctggaga cgaaacagaa cctgaagtgg
    10201 acgcaacagg gcagggacct gaagatcaca atgccggcat tcgaccccaa ccggatgacc
    10261 agcgaatatg cctatgtaat caagatcact tcgatatgaa aaaattggta ctggccgcgg
    10321 gactggcgct gatagtggcg aacgtctccg cgcagcagcg gatggatttc
//
LOCUS       DASYSV010000245         6474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1325437, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000245 DASYSV010000000
VERSION     DASYSV010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325437"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VGQ51_07560"
     CDS             <1..1403
                     /locus_tag="VGQ51_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9036458.1"
                     /translation="RQLIGQFFFDSILFATLATATAVVLLQFLMPVYSAFLGYELPSY
                     WGNVWFYVFLAGIILVAGVFAGSYPALLLSSFSPIESLKGKLKVGKRGAFFRKMLVVF
                     QFGTSVLLIVSVAVVMQQMHYVRNFDLGFDKAQQMIVRLDNNPIYDNKDGFKKAVQAD
                     PGVVSVSLMSGEPGGFHDFYGFESGARPGEKLRMNTEFSDFQYVKTLGLKIIAGRDLS
                     LPTDTDRSVLINRSAATALGLSPEQAIGKSMKNLSADSLPRMVAGVVEDYHYSSLKDM
                     IGALVISPKKGDRRLALIRLRGGDLQQSIDRIKKIYGRYAADYPFEYSFLDDQFDKLY
                     RSEGKQETLLTIFSTIAICVACLGLFGLACYTAVKRNKEIGVRKVLGSSVENIVLLLS
                     KDLLGPVVLGTAIALPAAYFITRQWLQGFAYRVALEWWLFLAAAAVGVVIALATVSVQ
                     AVKAAMANPTRALRSE"
     gene            1457..3826
                     /locus_tag="VGQ51_07565"
     CDS             1457..3826
                     /locus_tag="VGQ51_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036459.1"
                     /translation="MIKNYFLTAWRNMLKTKGYTALNVSGLAIGMAVALMIGLWVYDQ
                     YQYDRWLPDYQQLYQVRRNFNSNGDTLNFTSTSLRLADALRTQVPEVEYVAETDGSQG
                     HVLLVNGKQFMLTGYAIGGDFLKMFEFPLLEGNAATALRDPYSIVLTKHAAEALFGKE
                     EPMGRTIRIDNKNNVVVKGVVKDLPYNSSFQFDFIVPFKYSDLVHNYTVNGAAGSFGN
                     NGFSQYVRLKPGISYSQVAPKIADIEHTEKGNTNAMLSVVVLQPMANWHLYGNYINGK
                     ETGGFVDYVRMFTIIGVLVLLIACINFINLTTARSERRAKEVGVRKAIGSQRRDLIIQ
                     FLAESFLLTLLASLLALVLVQLALPSFNALTGTRVRIPASNLVFWMMLLGCVVVTALI
                     AGGRPAFILSSFNPVKVLKSGGAAGRTSTLSRKILVVAQFSCSIALIIGTIIVYRQIE
                     HAKERPTGLDTDRLMMTWMNDELRQNYTALKDELLEKGIAGSVTTATSPATDIYWHSD
                     VGQWPGKVAGETIEMGTILVGDDYFRTLGMGMAAGRDFGKADTLNVVFNEAAIAHMRI
                     KKPVGQVINWDTTRRIIGVAKNALMLSPFSPADPTMFIYAPEGSRNVMMYRLAPSIAT
                     QDAVARLTALFGRYNPSYPYKYSFADKSYAAKFNLEVLVGKLAGIFAALAIFISCLGL
                     FGLAAYMAEQRTREIGIRKVLGASVGQVWMLLSKDFMVLVGVSCLLASPLAFYFLHGW
                     LMKYEYRISIGAGVFVTAGVMALVITVVTISFQAVRAAVANPAKALRSE"
     gene            complement(3857..5257)
                     /gene="fumC"
                     /locus_tag="VGQ51_07570"
     CDS             complement(3857..5257)
                     /gene="fumC"
                     /locus_tag="VGQ51_07570"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEV9036460.1"
                     /translation="MEYRIEKDTMGEVKVPIDAYYGAQTQRSVENFPIAQDINKMPRE
                     IIRAFAHLKKAAALTNLEAGVLSKEKADLIGRVCDEILTGALDEWFPLVVWQTGSGTQ
                     SNMNVNEVIAYRGHVLAGGKLTDKEKTLHPNDDVNKSQSSNDTFPTAMHIAAYTILTG
                     TTIPGIQALRDTLAAKSKAFMKVVKIGRTHFMDATPLTLGQEFSGYVSQLDHGLKAIR
                     NSLAHLSELALGGTAVGTGINTPPNYAENVAKHIAALTGLPFITAPNKFEALAAHDSI
                     VEAHGALKTVAVSLMKIANDIRMLSSGPRSGIGEIHIPDNEPGSSIMPGKVNPTQCEA
                     LTMISAQVLGNDVAINIGGATGHFELNVFKPVMIYNFLHSARLIGDGCNSFNERCAKG
                     IEPIEKNIREHLNNSLMLVTSLNTKIGYYKAAEIAQKAHKEGTTLKEMAVKLGHLTAE
                     QFDEWVKPEEMVGKID"
     gene            5416..>6474
                     /locus_tag="VGQ51_07575"
     CDS             5416..>6474
                     /locus_tag="VGQ51_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV9036461.1"
                     /translation="MRHIKCLLAILFIAIAGPLAGRAQPIEKYEWKAGSGGGYSYRYV
                     SNDPFQARIYTLPNGLTVILSPNHKEPRVSVRIAVRAGSNTDPRDHTGLAHYLEHLLF
                     KGTETYGSLDWAKEKPYLDQIDALYEQYNSTKDTGQRKRIYHRIDSVSGVASHYAIAN
                     EYDKMMANIGSQGSNAHTWVEETVYEEDVPSNAIDKFLAVQAERFRDPVFRLFHTELE
                     AVYEEKNRTLDNDGSKMQQAMLYNLFPTHNYGQQTTIGTIQDLRNPSLRAIRAYYHKY
                     YVPNNMAVIMAGDFDPDALIGKIAQAFAYMQPKPVQLYNPAPERNLTAPIVKTVYGPS
                     AENMRICYRTPACMTRDAV"
BASE COUNT         1373 a         1463 c         2059 g         1579 t
ORIGIN      
        1 ggagacagct gatagggcag tttttctttg actcgatact ttttgccacg ttggctacgg
       61 cgacagcggt agtccttttg caatttttga tgcctgtcta tagcgccttc ctgggatatg
      121 agttgccttc atattgggga aatgtgtggt tctatgtttt tttggcgggc atcattctcg
      181 tcgcgggggt gttcgcgggc agctatcccg cgctgctgct gtcttccttc tccccgatag
      241 aatcgttgaa agggaagttg aaggtgggga agaggggggc gttcttccgg aagatgctgg
      301 tggtttttca gtttgggaca tcggtgttgc tgatcgtcag cgtggcggta gtgatgcagc
      361 aaatgcacta tgtacgcaac ttcgacctgg ggttcgacaa ggcacagcag atgatcgttc
      421 gtctcgataa taatccgatc tatgacaata aggacgggtt caagaaggcg gtacaagccg
      481 accccggggt agtaagtgtc tcgctgatgt cgggcgagcc gggcggtttt catgattttt
      541 atggcttcga gtccggggcc agaccgggag agaagctgag gatgaatact gaattctcgg
      601 atttccagta tgtaaaaacg ttggggctga agatcatcgc gggcagggat ctgtcccttc
      661 ccacggatac ggacagatcg gtgctcatca accggagcgc tgccacggcg ttgggtctga
      721 gcccggaaca ggctatcgga aaatcaatga aaaatctttc cgcggacagt ctgccgagga
      781 tggttgccgg agttgtggag gactatcatt attcttcatt gaaggatatg atcggggctt
      841 tggtgatctc tccgaaaaag ggggaccggc ggctcgcact gatcaggttg cgaggcggcg
      901 atctgcagca atccatcgac aggatcaaga agatctatgg ccggtatgcc gccgactatc
      961 cgtttgaata ttcgttcctc gacgatcaat tcgataagtt gtaccggagt gaaggcaagc
     1021 aggagaccct gcttaccatc ttttccacga tcgccatctg tgtggcctgt ctggggcttt
     1081 tcgggcttgc ctgttatacg gctgtcaaac gcaataagga aataggtgta aggaaggtgc
     1141 tgggctcttc ggtagagaat atcgtattgt tgttgtcgaa ggacctgttg ggcccggtgg
     1201 tgctggggac ggctatcgct ttgcctgcgg cgtattttat tacgagacag tggctgcagg
     1261 ggtttgcgta ccgggtggca ctggaatggt ggttgttttt ggcggcggcg gcggtgggag
     1321 tggtgatcgc gctggctacg gtgagtgtac aggcggtgaa ggcggcaatg gcgaatccga
     1381 cgagggcgct gcggagtgaa tagctgcgaa gacgttagac cgggggtgaa agaccgggag
     1441 cgaatagtag atttttatga taaagaatta ttttctcact gcatggcgca atatgctcaa
     1501 gacaaagggc tataccgcgc tgaatgtttc gggtctggcc atcgggatgg ccgttgcgct
     1561 gatgatcggt ctttgggtat atgatcaata tcaatatgac cggtggctgc cggattatca
     1621 gcagctttac caggtgcgcc gcaatttcaa cagtaacgga gacacgctca attttacttc
     1681 tacctcgctg aggctcgcgg atgcgcttcg tacgcaggtg cccgaggtgg aatatgtcgc
     1741 agagacggat ggcagtcaag ggcatgtgct gctggtgaac ggtaaacaat ttatgctcac
     1801 gggttatgcc attggcggcg attttttaaa aatgttcgaa ttcccgttgc tggagggcaa
     1861 tgcggctacg gcactgcggg acccgtattc gatcgtattg acgaagcatg ccgcggaggc
     1921 gctgtttggc aaagaggaac cgatgggaag gactattcgc atcgataaca agaataatgt
     1981 cgtcgtgaaa ggtgtggtga aggacctgcc ctataactcc agttttcaat tcgacttcat
     2041 cgtccctttc aagtatagcg acctggtgca caactacact gtcaacggag ccgcggggtc
     2101 gttcggcaat aacgggttta gtcaatatgt acgattgaaa ccggggatat cctactcgca
     2161 ggtggcgccg aagatcgcgg atatcgagca taccgaaaag ggcaatacga atgcgatgct
     2221 ttcggtcgtg gtattgcagc cgatggcgaa ctggcatctt tatggtaatt atatcaatgg
     2281 caaggaaacg ggcggtttcg tggattatgt ccggatgttc acgatcattg gcgtgctcgt
     2341 gctcctgatt gcgtgtatca atttcatcaa tctgaccact gcgcggtccg agcggcgggc
     2401 gaaggaagta ggagtgcgga aagccatcgg atcgcaacgc agggatctga tcattcaatt
     2461 cctggcggag tcgttcctgc tgaccttgct ggcttcgctg ctggcgctgg tgctggtgca
     2521 actggcgctg ccttctttta atgcgctcac gggcaccagg gtgaggatac cggcgtcgaa
     2581 tcttgtattc tggatgatgt tgttgggttg tgtggtagtg accgcgttga tcgccggggg
     2641 cagacccgcc tttatcctgt cttctttcaa cccggtgaag gtgttgaaaa gcggaggggc
     2701 agctggccgg acgagtacgc tttcgaggaa gatcctggtc gtagcccagt tcagctgcag
     2761 catcgcgttg atcatcggta cgatcatcgt ttaccggcag atagaacacg cgaaggaaag
     2821 accgacgggt ctcgatacgg acagactgat gatgacctgg atgaatgacg agctgcgcca
     2881 aaattatacg gcgttgaagg atgagcttct tgaaaagggc attgcgggaa gtgtgacgac
     2941 ggctacgagt ccggcgaccg acatttactg gcattcggat gtggggcagt ggccgggaaa
     3001 ggtggcgggc gagactatcg agatggggac gatccttgtt ggggacgatt atttcaggac
     3061 actgggcatg gggatggctg cggggcggga tttcgggaag gcggatacgc tgaatgtggt
     3121 gtttaatgag gcggccatcg cgcatatgcg gataaagaaa cccgttggcc aggtgatcaa
     3181 ctgggatacg actcgacgga tcatcggagt ggcaaagaat gcgttgatgt tgtcgccttt
     3241 ttcacctgcc gatccgacca tgttcatcta tgcccccgaa ggctcacgaa atgtaatgat
     3301 gtaccggctg gcgccgtcga tcgcgacgca ggatgctgtt gcgcggctga ccgcgttgtt
     3361 cggaagatat aatccttctt atccttataa gtattcgttc gcagataaga gttatgccgc
     3421 gaaatttaat ttggaagtgc tggtcgggaa gctggcgggg atctttgccg cgctggcgat
     3481 ctttatttcc tgtctggggt tgttcgggct ggcggcttat atggcggagc aaaggacaag
     3541 ggagatcggg atacggaagg tgttgggggc gagtgtgggg caggtgtgga tgttgttgtc
     3601 gaaggatttt atggtgttgg tgggggtgag ctgtttgctc gcttcgccgc tggcgtttta
     3661 ttttctgcat gggtggttga tgaaatacga gtatcgaatc tcgatagggg cgggtgtgtt
     3721 tgttacggcc ggggtcatgg cactggtgat caccgttgtt acgataagtt ttcaggccgt
     3781 gagggcggcg gtggccaatc cggcgaaggc gctccggagt gagtagctcc ggagacatta
     3841 gctccggagc gagtagttag tcgatctttc ccaccatctc ttcgggtttc acccattcgt
     3901 cgaattgctc ggcggtgagg tggccgagct tcacggccat ctctttgaga gtggtccctt
     3961 ctttgtgtgc tttttgggcg atctcggcgg ccttgtagta gccgatcttc gtattgagcg
     4021 aggtgacgag catcagcgaa ttgttgagat gctcgcggat attcttttcg atgggctcga
     4081 tgcctttggc gcagcgttcg ttgaagctgt tgcagccgtc gccgatgagc cgtgcgctgt
     4141 ggaggaaatt gtagatcatg acgggtttga agacgttgag ctcgaagtga ccggtggcgc
     4201 cgccgatgtt gatggcgacg tcattgccga gcacctgcgc ggagatcatg gtcagcgcct
     4261 cgcattgggt ggggttcacc ttgccgggca tgatcgaaga gccgggttcg ttgtcgggaa
     4321 tatggatctc gccgatgccg ctgcgggggc cggaggacag catacggatg tcgttggcga
     4381 tcttcatcag gctgacggcg acggttttga gggcgccatg ggcttcgact atgctgtcgt
     4441 gagccgcgag ggcctcgaat ttgttcgggg cggtgatgaa cggcagaccg gtgagcgcgg
     4501 caatatgttt tgcaacattc tctgcgtagt tgggaggggt attgatgcct gtgccgacgg
     4561 cggtgccgcc gagggcgagt tcggagagat gggcgagcga attgcggatg gctttgagac
     4621 catggtcgag ctgggagaca tagccgctga attcctgacc gagagtgagg ggagtggcgt
     4681 ccataaagtg cgtacggccg atcttcacca ccttcataaa ggctttgctt ttggcggcaa
     4741 gcgtatcgcg cagcgcctgg atgccgggta tggtcgtgcc cgtcaggatc gtataggcgg
     4801 cgatatgcat ggcagtgggg aaggtgtcat ttgaggactg ggacttgttg acgtcgtcat
     4861 tgggatgaag agtcttttcc ttgtcggtga gcttcccacc ggcgagtacg tgtccgcggt
     4921 acgcaatgac ttcattgaca ttcatattgg actgggtgcc gctgcccgtc tgccatacca
     4981 caaggggaaa ccactcgtcg agggcaccgg taaggatctc gtcgcagacg cggccgatga
     5041 gatcggcttt ttctttggaa agaactcccg cttcgagatt ggtgagtgcg gccgcctttt
     5101 tcagatgagc gaaggcgcgg atgatctcgc ggggcatttt attgatatcc tgtgcgatgg
     5161 ggaagttctc tacactgcgt tgggtctgag cgccataata ggcatcgatg ggtactttta
     5221 cctcacccat cgtatccttt tcgatccggt attccatggt aatgattgtt ggaggggcaa
     5281 agttaggctg aatgaggata tccggcaaaa aagagcggag atgaaagttg gtcgtgggtg
     5341 aaaaactgga cgggcgcctg ttaaaattgg atgtacggga tgggtaaaat ggaggaaatt
     5401 cagttgattt gctatatgag acacattaaa tgccttcttg caatattgtt tattgccatt
     5461 gcgggcccgc tggcgggccg ggcgcagcct atcgaaaaat acgaatggaa agcgggcagc
     5521 ggcggcggat attcttacag gtatgtcagc aatgacccct tccaggcccg gatctacacg
     5581 ttgcccaacg ggctcaccgt aatcctgagt cccaatcaca aggagccgcg ggtgtcggtg
     5641 cgtatcgcgg tccgtgccgg aagcaatacc gatccgaggg atcatacggg tctcgcgcac
     5701 tatctggagc acctgttgtt caagggcacg gaaacatatg gatcgctgga ctgggcaaag
     5761 gaaaagccct atctggacca gatagatgcc ctgtatgagc aatacaattc cacgaaggat
     5821 acaggtcagc gcaaacgtat ctatcatcgt atcgacagcg tgagcggtgt cgcatcgcat
     5881 tatgccatcg ccaacgaata tgacaaaatg atggccaata tcggctcgca gggcagcaat
     5941 gcccacacct gggtagagga gacggtatac gaagaggacg tcccttcgaa tgccatcgat
     6001 aaattcctgg ccgtccaggc ggagcgattc cgtgacccgg tgttccggct tttccatacc
     6061 gagctggagg ccgtctatga agaaaagaac cgcacgctcg acaatgacgg ctcgaagatg
     6121 cagcaggcta tgctctacaa tcttttcccg acccacaact acggacaaca aaccaccatc
     6181 ggtacgatac aggatctcag gaacccgtcg ttgagagcga tcagggctta ttaccataaa
     6241 tattatgttc cgaacaatat ggccgtgatc atggcgggcg atttcgatcc ggatgcgttg
     6301 atcggcaaga tcgcacaggc ttttgcctat atgcagccca aaccggtgca gttgtataat
     6361 cccgctcccg agcgtaatct tacggccccg atcgtcaaga ccgtctatgg acccagcgcg
     6421 gaaaatatgc ggatctgcta ccggacgccg gcctgcatga cgcgcgatgc cgtc
//
LOCUS       DASYSV010000246        12096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2220192, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000246 DASYSV010000000
VERSION     DASYSV010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12096
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220192"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VGQ51_07580"
     CDS             complement(<1..1500)
                     /locus_tag="VGQ51_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV9036462.1"
                     /translation="MSTTTRRAFLKNTGITGIALTLGIRFPAFAAADTTAGLPDIVSA
                     SGTVPEGIGLMTWISIDKSGKVTIFSHRAEMGQGAFQVIPQMIAEELEVDLDQVNIVF
                     APASQTKYGSQITGGSSTVRGSYKHLLRIGASAREMLIGTAAKKWGVPASECYAEKGH
                     VIHRPTGKKFTYGDLVEEAAKLDPPKNPPQKQRKDYRVIGQPLPRQDTPLKTNGTAVF
                     GLDKKLPGMLYAVVERSPRILGKVKSFDDSAAKAVPGVRHVLKVQRTVFSRLQEGVAV
                     VADSIWTATQARKLLRIEWDDTGFEHLDTDTLYARMRADLDKPGLNDRSAGDFDEAFR
                     DADLKIEAVYEAPYESHACMEPLNCIAHVRENAIEIWGPIQGPDWVQSDLSRRMGVPV
                     ENVVVNMTFLGGGFGRKAFLDYPHEAAFISKAVQAPVQVVWTREDDMTQGPFRPGGVY
                     GCKAGLTKGGSVQAFQTKTAIQNMDQQGAGADRHAYNSSTTEGLCKPWFD"
     gene            complement(1511..1978)
                     /locus_tag="VGQ51_07585"
     CDS             complement(1511..1978)
                     /locus_tag="VGQ51_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV9036463.1"
                     /translation="MSSYTLLINGKKYPIEADAKMPLLWAIRDLVGLTGTKYGCGVAQ
                     CGACTVLLNGNPVRSCSIPASMVANQPITTIEGLSTDNSHPVQKAWITEQVPQCGYCQ
                     SGQILAAAALLKKNPHPTDKDIDIAMQGNLCRCGTYPRIRKAIHTAVANASHQ"
     gene            2180..2728
                     /locus_tag="VGQ51_07590"
     CDS             2180..2728
                     /locus_tag="VGQ51_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036464.1"
                     /translation="MKTLFITLLSLGVAMGASAQRGGHVIAHGGGGFHHAPVYHHHYF
                     YPRSYVGVGFGFGYPWGYGFYNPWGWGPMYGYGAYPPYYYGHGSTPPQLAGEIQGIKD
                     DYGQQIKDVKHDKALTHKEKRAKIDQLKLDRDATVTKARHDYYFNSRRNYEKNDQAPQ
                     KNNNSSGDEPGYQDKGSGDTGQ"
     gene            2790..3590
                     /locus_tag="VGQ51_07595"
     CDS             2790..3590
                     /locus_tag="VGQ51_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV9036465.1"
                     /translation="MKNFTNQVIWITGASSGIGEALAYAFSAAGARLLLSSRRTEELE
                     RVRKNCAHPESARVLPMDLLDHASFGSHVKEAIGAFGQVDILIHNGGISQRGTVAETD
                     MSVHRQVMELDYFSYVALTQALLPHFMERRSGRFVVVSSVMGKIGTPMRSAYAAAKHA
                     LHGFFDCLRAEMAPMNIGVTVLTPGYIQTNITKHALTKDGTPFGQVSQNIEKGLPAEK
                     AAKQILKAIGRGAFEAYIGKPGGERVALLLNRMTPGVLMRLAPKLQPK"
     gene            3600..6083
                     /locus_tag="VGQ51_07600"
     CDS             3600..6083
                     /locus_tag="VGQ51_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9036466.1"
                     /translation="MFYSYLKTAWRTLFRHKGATLIKLTGLSIGMACCLLIVVYIDDE
                     LSYNSFHAHYPEIYRVNFVKHEDGETRVMSGTPNSAGPAIAKDLPQVAAVGRRYTRAG
                     ILEVKDDGGIDSPGKAMHGSMRRRFQEPNIDFADEGLLDVLTVHFVQGNISDALSAPG
                     SIVITRDMARKYFGREDVVGRTMVYENSTALRVAGVIDSWPANSDLHSDAMISFETLY
                     SVETKGVADFLRTNWLYNPAETYVRLKPGVAASAVAPALAQLTRKYGDQRVQKYWQLA
                     LQPLSEVHLYASNVEGNPSTNSITYIYIFSAIALLILLIAVVNFINLSNAQSLTRIAE
                     ISIRKVSGAGVRQLLVQFLGEGLLLSLLAFVVALALMAPGLRLLDVMTGKDLGIAVLG
                     RGWILLVFVGLLLGTGLLAGIYPALFITRMELVGLLKGKTGGWGRSRIRQSLIVTQFV
                     IAIALMVGAIVIQRQLGYLRNKPLGFDKEQLLVLPLFGKNPSPINSGVDGPLRARMNA
                     FENDLRANASVRGVTVSSVLPGDFFVSGLVIPEGHVGQDNIFVPWASVDYDFVSTIGI
                     PVVAGRDFSKATGTDHLQAFIINESALRTFGWTSAQEAIGKTMIRGDATTGKKGHVIG
                     VVKDFNFGRLDRPQEPMIMDVNVPRFTEFAVRVAPVGVPGTIAAIRRLWDQYFPERVF
                     EYSFLDSNRNALYNAQENLSLLVRYFAVIAIFLSCTGVFGLASFMAMQRTREIGIRKV
                     LGASVGGLVALLYRDFLRLVVVALVVAVPLSWWAMNKWLHDFAYRIGLSWWIFAAVGG
                     VAVFLSFFTVGIQGLRAARVNPVESLRSE"
     gene            6343..7326
                     /locus_tag="VGQ51_07605"
     CDS             6343..7326
                     /locus_tag="VGQ51_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV9036467.1"
                     /translation="MKQNLKEVHPRAGGIDVGSKSFYVSAEEQEVRVFPTFTSDCQAL
                     RDYLLSDVINDITGASGLRILAAILGGERDAEKLTELCVSQIKDNKRELVYKSLEGDW
                     QEEHLFALQQAFTTWEHYNELIRACDKAVEKQLSAMTEGKPKIERVSKRKPIRYHRPE
                     IENLHEPLLQLTEGKDPTGIPGLTDYSFLQIVSEVGTDMRAWPTEKHFASWLKLAPMR
                     SSSGKMIKRVTLKRHNKAGQLFRSLAQGILTSKYLALGVFGRKIRARRGGAAAIKAIA
                     RKIACYYYRMMTKGGDFVDQGIAAYEQQLQNRKRRYLEKMAAKLNLQLVPA"
     gene            complement(7539..9194)
                     /locus_tag="VGQ51_07610"
     CDS             complement(7539..9194)
                     /locus_tag="VGQ51_07610"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV9036468.1"
                     /translation="MPKLLLRTLTIIALLPFARLTAQPYNPAIDVQHYNFQLWLNDSS
                     DRIRGDAGITLEFREPANEFHLDLTGKNADGKGMTVTSVREGDKPVPFTQQDDQLILH
                     AGGAPGSSRTYLIDYEGIPADGLIISKNKFGSRGFFGDNWPNRAHNWLPCVDDPSDKA
                     TLDFSIVSPDHYTVVANGALQEEKPLANHLKLTRWKESEPIAPKIMVIGVADFAVDHP
                     GDAMGIPVWNYVYRQNKEAGFRSYAVALDILPFYITHIGPYPFEKEGNVQSKTRFGGL
                     ENASAIFYYEGSVGSPGVESLMAHEIAHQWFGDAATETAWRHLWLSEGFATYMTLCYL
                     ENKYGPDTLRTSLRKDRAKVIAFEKRRFTPVVDTGVHSDYMQLLNPNSYEKGGWALHM
                     LRRTLGDATFWKGISAYYSAYRNKNASSANLERIMDSVSNKDLREFFHEWLDIPGHPH
                     VTISWKYDPDKRSLAIDFTQHDHDPKSAGAAQSTNAATANPIPSADPLFIFPLEYSVD
                     GTLHHVDIHDRLTHVDIPLPQKPSNITPDPNTNLLADFEVTER"
     gene            complement(9226..11436)
                     /locus_tag="VGQ51_07615"
     CDS             complement(9226..11436)
                     /locus_tag="VGQ51_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV9036469.1"
                     /translation="MPEHKIARREFLKHTGFSAAALILGISVYNDAEAAIAKLHAAAL
                     PLAGLPDITGAGGLPEIANITPYIIIEPTGRITLINPKPEMGQGTYQSIPALIAEELE
                     VSLEGTTILTSWGQKDIPGQSAGGSSSVRGSYTQLRKVGAAAREMLRTAASKQWNVPI
                     EECKADNGKILHQPTGRFLGYGDLVAAASKLEVPTNPPLKDPKDFKILGKSMPRMDIP
                     LKVTGKAVYGIDTELPGMVYASVERCPVFGARLVSYDDSATLKVKGVTTTVKAQRVLG
                     KNHYEGIAVIADNYWAALKGRRALKVQWDYQGFDKFNSNDYEQHLRDLAKTEGVLAHD
                     SGDFDGAMASAPHKLEALYETPIVAHATMEPMNCLAHWQEGDKVDIWTSVQGAGIVTR
                     ELSQTLGIPAENITAHISFVGGGFGRRLAADYAAEAAGISKAIGKPVKVLWTREDDIQ
                     LGPFRPMTFSAMKGAVSAEGKAVAFQHKVISPSISATRSDKYDKTKPDRSMTEGISDQ
                     DYEFPNMKNAYVFADIHVPMQPWRSVTSSTLAFAHECFIDEMAAAAGKDPMDFRLSLL
                     TKESDTRRVLTHLREVSHWDKPLPKGRGRGVAQYKFFAGLCANVIEVSTKKDGSLKVD
                     KVCSVIDLGTVVNPDTVRAQVEGAIVMGLVAAIKDGIRFEGGKSVQTNFHNNRMLRIN
                     EMPAVEVHILAEGGPVMKGVGEPGLPPVAPALANAVFAATGKRIRRLPFDLAKV"
     gene            complement(11429..11929)
                     /locus_tag="VGQ51_07620"
     CDS             complement(11429..11929)
                     /locus_tag="VGQ51_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV9036470.1"
                     /translation="MPYSLTINNKPVQVDAEPDMPLLWVIRDLIGLKGTKFGCGIAQC
                     GACTVHLGSSAVRSCGIPISAAEGQKITTIEGLTGDKVGESVQKAWIDENVPQCGYCQ
                     SGQIMSAVALLRHNPKPTDADIDIAMQGNICRCGCYDRIRKAIHLAAAAAKPAPSRLQ
                     KPLIHA"
BASE COUNT         2434 a         2805 c         3991 g         2866 t
ORIGIN      
        1 gtcgaaccag ggtttgcata agccttccgt ggtgctgctg ttgtaggcgt ggcggtcggc
       61 tcctgcgccc tgttggtcca tgttctggat ggccgttttg gtctggaagg cctgtacgga
      121 gcctcctttc gtgagacccg ccttgcagcc atagacgccg ccggggcgga agggcccctg
      181 tgtcatatcg tcctcgcgtg tccagacgac ctgtaccggc gcctggacgg ccttcgaaat
      241 aaaagcagcc tcgtgaggat agtccaggaa agccttgcgg ccgaaaccgc cgccgaggaa
      301 ggtcatgttt acgaccacgt tttccacggg cacgcccata cgccggctca gatcggattg
      361 tacccaatcg gggccctgta tgggtcccca gatctcgatg gcattttccc tgacgtgggc
      421 gatgcaattg aggggttcca tacaggcatg ggactcgtag ggagcttcat atacggcctc
      481 gatctttaaa tcggcgtccc tgaaggcctc gtcgaagtcg cctgcgctac ggtcattgag
      541 accgggtttg tcgagatcgg cgcgcatccg ggcatatagc gtatcggtgt cgaggtgttc
      601 gaagccggtg tcatcccatt ctatcctgag caacttgcgg gcttgcgtgg cagtccaaat
      661 gctatctgcg acgacggcta cgccttcctg gagacgcgag aagacggttc gttgaacttt
      721 gagcacatgg cggacgccgg ggacggcctt cgccgccgag tcatcgaagc ttttcacttt
      781 tccgaggatg cgggggctgc gttcgacgac ggcgtagagc atgccgggaa gttttttatc
      841 gagaccgaag acggctgtac cgtttgtttt caaaggggta tcctggcggg gaagcggttg
      901 gccgatgacc cggtagtcct tgcgttgttt ttgcggcggg tttttgggcg gatcgagctt
      961 tgccgcctct tcgacgagat cgccgtaggt gaattttttt ccggtgggcc ggtggatgac
     1021 atggcctttt tccgcatagc attccgatgc cggcaccccc cattttttgg cggcggtgcc
     1081 gatgagcatt tcacgcgccg acgcaccgat ccggagcagg tgtttgtagg aaccgcggac
     1141 ggtagagctg ccgcctgtga tctggctgcc atatttggtt tggctggcgg gggcgaagac
     1201 gatattgacc tggtcgaggt ctacttccag ctcttcggcg atcatttgtg gtatcacctg
     1261 gaacgcgccc tgtcccattt ccgcgcggtg gctgaatatg gtgaccttgc cggatttgtc
     1321 gatggatatc catgtcatca gtccgatgcc ttcgggaaca gtgccggagg ctgagacgat
     1381 gtctgggagg ccggcggttg tgtcggcggc ggcgaaggca ggaaaccgga tgccgagggt
     1441 cagcgcaatg ccggtaatgc cggtattttt aagaaaggct cgacgggtag tggtagacat
     1501 aaccgttagt ttattggtga gaagcgttgg cgacggccgt atggatggct ttgcggatcc
     1561 gggggtaggt gccgcagcgg cagaggtttc cctgcatcgc gatgtcgata tccttatcag
     1621 tgggatgggg attctttttc aacagcgcag ccgctgcgag gatctggccg ctctggcaat
     1681 agccgcattg gggaacctgt tcggtgatcc aggctttttg cacggggtga gaattgtccg
     1741 tcgagagacc ttcgatggtt gttatcggct ggtttgcgac catggacgcc ggaatggagc
     1801 aggagcggac ggggttgcca ttgagcaaaa ctgtacaggc gccgcattgt gctactccgc
     1861 agccatattt cgttccggtg agaccgacga gatcgcggat ggcccaaaga aggggcattt
     1921 ttgcgtccgc ctcgatggga tattttttcc cgttgatcaa taaagtgtag gaagacatga
     1981 tggtgaattt tggcagggag aagttacgac attttttcca aatgctgtct gggtgacagg
     2041 gcaggcgggg agcgcgaagg agcaaagctg gcgtgcaggg gcagctttgt tatagctatg
     2101 ttataaacgc cgaatccgtt taaccttgaa tgatatttgc cttcttagta ccgaacgtac
     2161 aacgaaaaaa ggagaagata tgaaaacact attcatcaca ttactttcac tcggtgtcgc
     2221 catgggcgcc tcggcccagc gtggcgggca cgttatagcg catggcgggg gagggttcca
     2281 tcatgcgccg gtatatcatc atcactattt ctatccgagg tcttatgtag gggtagggtt
     2341 tggattcggg tacccgtggg gctatggttt ttataatccc tggggctggg gtccgatgta
     2401 tgggtatggg gcttatcctc cgtactatta tggccatggc tccacgccgc cccaactggc
     2461 cggagagatc cagggtatca aggatgacta tgggcagcag ataaaggacg tcaaacatga
     2521 caaggcgctg acccataaag aaaagagggc taagatcgat cagctgaagc tggaccggga
     2581 tgccaccgtc accaaggcaa ggcatgatta ctattttaat tcccggagga attatgaaaa
     2641 gaacgatcag gcgccgcaga agaataacaa ctcttcgggc gacgaaccgg gataccagga
     2701 caagggttcg ggggacacgg gtcaataata cttttgaaaa atcaacaagc gagggccatc
     2761 cggtgggatg gctttcgtat ttttaacaca tgaagaattt taccaaccag gtcatatgga
     2821 tcacgggcgc atcatccggc atcggggagg ctttggctta tgcattttcg gcggccggtg
     2881 cccggctttt gttatcgagc aggcggacgg aagagctgga aagggtcagg aagaattgcg
     2941 cgcatccgga atccgcgcgg gtgctgccga tggacctgct ggaccatgct tcattcgggt
     3001 cgcacgtaaa ggaagctatc ggggccttcg gccaggtgga tatcctgatc cataacggtg
     3061 gtataagtca gcggggcacg gttgcggaga cggatatgtc ggtccaccgg caggtgatgg
     3121 aactggatta tttcagctat gtagcgttga cgcaggcgct gctgccacat tttatggagc
     3181 ggcggagcgg ccgtttcgtc gtcgtgagca gcgtcatggg caagatcggc acgccgatgc
     3241 gttctgccta tgcagccgcc aaacatgccc tgcatggatt tttcgattgt ctgcgggcag
     3301 agatggcgcc gatgaatatc ggagtgacgg tgttgacgcc ggggtatatt cagaccaata
     3361 taacgaaaca tgctctgacc aaagacggca caccgttcgg tcaggtatcg cagaacatcg
     3421 agaaagggtt gcccgcggag aaggcggcga agcaaatatt gaaagcgata gggcgcgggg
     3481 cattcgaggc ctatatcggg aaaccgggcg gggaacgggt ggcgctgtta ttgaacagga
     3541 tgacgccggg agtgctgatg cggctagcgc caaagctgca gccgaaataa gggggcatta
     3601 tgttctatag ctacttaaaa acggcgtgga gaaccctgtt ccgccataag ggcgccacac
     3661 tgatcaaact gacaggactt tcgataggga tggcctgctg tctgctgatc gtcgtctata
     3721 tcgacgatga gttgagttat aacagctttc acgcgcatta cccggagatc taccgcgtga
     3781 atttcgtcaa acatgaggat ggggaaacgc gggtgatgtc gggaacgccc aattcggcgg
     3841 gaccggctat cgccaaagac ctgccgcagg tggcggctgt ggggagacgg tatacccgtg
     3901 cggggatatt ggaggtgaaa gacgacggag ggatcgattc gccggggaag gcgatgcatg
     3961 gatcgatgcg gaggcggttc caggagccga atatcgattt tgccgatgaa ggtttgctgg
     4021 atgtactgac ggtgcatttt gtgcagggga atatttcgga cgccttgtct gcgccaggat
     4081 cgatcgtcat cacccgggac atggcgcgca aatattttgg ccgggaggat gtcgtgggcc
     4141 ggacgatggt ctatgaaaat tcgacggcat tgcgggtggc gggagtcatc gattcctggc
     4201 cggccaactc cgatctgcat tcggatgcca tgatctcgtt cgagacgttg tatagcgtcg
     4261 aaacgaaggg cgtagccgat ttcctgcgaa ccaactggct gtacaatcct gcagagactt
     4321 atgtccgtct caaaccggga gttgccgctt cggctgtcgc gcctgcgctg gcgcagctga
     4381 cccgtaaata tggcgaccag cgggtacaga aatactggca gcttgcgctt cagccgttgt
     4441 cggaagtaca cctgtacgct tcgaatgtgg aagggaaccc gagcaccaac agcatcacct
     4501 atatctatat cttttcggcg atagcactgc tgatcctgct gattgcggtc gttaatttta
     4561 tcaatctgtc caatgcgcag tcgctgacgc gtatcgccga gatcagcatc cgcaaggtgt
     4621 cgggtgcggg tgtgcgtcaa ttgctggtac aattcctcgg ggaaggattg ttgttgagtc
     4681 ttctagcctt tgtggtggcg ctggcattga tggcgcccgg gttgcggctg ttggatgtca
     4741 tgacgggtaa ggatctgggt atcgcagtgc tgggcagggg ctggatattg ttggtgtttg
     4801 tcggattgtt attgggcacc gggctgttgg cgggcatcta tccggcgttg ttcattacgc
     4861 ggatggaact ggtaggattg ttgaagggca agacaggcgg gtggggcaga agccggatcc
     4921 ggcaatcgct gatcgtgacg cagtttgtca tcgcgatagc tctgatggtc ggcgctatcg
     4981 tcatccagcg gcagctgggc tatctgcgaa acaagccgtt gggtttcgac aaggagcagt
     5041 tgttggtatt gccattgttc ggcaagaacc cgtcgccgat caatagtggc gtcgatgggc
     5101 cgctgagagc gcggatgaat gcctttgaga atgacttgcg ggccaacgcg tcggtgcggg
     5161 gtgtgaccgt ttcgtcggta ttgccgggag atttttttgt cagcggtctc gtgataccgg
     5221 aggggcacgt cgggcaggac aatatttttg tcccctgggc atcggtggat tatgattttg
     5281 tgtccaccat cggcattccg gtggtcgcgg ggcgggattt ttcaaaagcg acgggtaccg
     5341 atcatctgca ggcttttatc atcaatgaat cggcattgcg caccttcggc tggaccagcg
     5401 ctcaggaggc catcggaaaa acgatgatcc gcggggatgc cacgaccggg aagaagggac
     5461 atgtaatagg ggtggtgaag gacttcaatt tcgggcggct ggacaggccg caggagccga
     5521 tgatcatgga tgtgaacgtg ccacggttta cggagtttgc ggtgcgggtt gcgcctgtcg
     5581 gggtgccggg gacgattgca gcaatccgga ggttgtggga tcaatatttt ccggagcggg
     5641 tgttcgaata ttcgtttttg gactcgaata gaaacgcgct gtataatgcg caggaaaacc
     5701 tgagcctgct ggtgcggtat ttcgcggtga tcgccatctt tctttcctgt accggtgtgt
     5761 ttgggctggc ctcttttatg gcgatgcaga ggacaaggga gatcggcatc cggaaggtgc
     5821 tgggggcgag cgttggaggg ctggtcgcgt tgttataccg ggattttttg cggctggtgg
     5881 tggtcgcgct ggtggtcgct gtgccgctgt cgtggtgggc gatgaataaa tggttacatg
     5941 attttgcgta tcggataggg ttgagctggt ggatctttgc ggcggtgggc ggggtggcgg
     6001 tgtttttatc gttctttacg gtggggatac aggggttgag ggcggcgagg gtgaatccgg
     6061 tggaatcttt gcgtagtgaa taggtccttc ctgactgcgt tagggtaggc ctacccgtct
     6121 gtcggcggta agagcgggaa gggacatgaa gaagggttac cttggggttc agctgataag
     6181 gaagtaacag cgctacgatc tagtgctaat tacctgctag ggaatcttgt tccggctgtt
     6241 atatgaaaaa aatattggcg ataccggcag cgatattccc gttcaggatg ctatcaaaca
     6301 gtcaacagcc ggccttgtta gctgatttta aaaccatcag caatgaagca aaacttaaaa
     6361 gaagtacatc cccgggccgg ggggatcgac gtagggagca aaagttttta tgtatcggcc
     6421 gaagagcagg aggtgcgggt attccccacc tttacatcag attgccaggc acttcgggat
     6481 taccttttgt ccgatgtgat caatgatatt accggggcca gtgggttgcg gatattggcc
     6541 gcgatcttag gcggggagag ggatgcggaa aagttgacgg aactgtgtgt atcgcagata
     6601 aaagataata aaagagaact ggtgtataaa tcgctggaag gggattggca ggaagagcac
     6661 ttgtttgctt tgcagcaagc atttacgacc tgggagcatt acaatgagct gatccgggcc
     6721 tgcgacaaag cggtggaaaa gcaattgtcg gcgatgacgg aaggcaaacc gaaaatagag
     6781 cgtgtgtcaa agcgtaagcc gatccgctat catcggcctg agatcgagaa cctgcatgag
     6841 cccttactcc agttgactga aggcaaggat cccaccggta ttcccgggct taccgactac
     6901 agctttttgc agattgtcag tgaggtagga acggatatga gggcctggcc gacggaaaag
     6961 cattttgcca gttggctgaa gctggcgccg atgcgatcca gctcagggaa aatgatcaaa
     7021 cgagtaacgt taaaacggca taataaggca gggcaattgt tccgaagttt agcccagggg
     7081 attttgacca gcaagtatct ggcgttgggt gtttttgggc ggaaaatccg ggccaggcgg
     7141 ggaggggccg cggctatcaa ggccatagcc aggaaaatag cctgctatta ttaccgaatg
     7201 atgaccaaag gtggggattt tgtagatcag ggcattgcag cttatgagca gcaattacag
     7261 aacaggaagc gaaggtatct tgagaaaatg gcggcaaagc tcaacctgca actcgtgccc
     7321 gcgtaatgtg tcctcaggat cggcaggagc gccgtgcgcg acagagagcc cgacctgccg
     7381 caagtggttt tacctggcgg cggggaacgc ccaaattaag gttggaggcg gcccggggtg
     7441 tgggtgtgtt ttttgaaaga aaattggaac cgaaatcaag ttgttgaaaa tcacgcaact
     7501 aattttggtt ccaaaaaact gatcgggaag aaggaccatt atctctcggt gacctcgaaa
     7561 tcggcgagga gattggtatt ggggtcgggg gtgatgttgg agggtttttg gggcagtggg
     7621 atgtcgacgt gggtgaggcg gtcgtggatg tcgacatggt ggagcgtgcc atcgacggag
     7681 tattcgaggg ggaagatgaa gagggggtcg gcggagggga tggggttggc ggtcgcggcg
     7741 ttggtggact gggcggcgcc ggcggatttg gggtcgtggt cgtgctgggt gaagtcgatg
     7801 gcgagggacc gtttgtcggg gtcgtatttc caggatatcg tgacgtgggg gtggccgggg
     7861 atgtcgagcc attcatggaa gaactctcgg aggtccttgt tgctaacgga gtccattatg
     7921 cgctcaaggt tggcgctgga ggcgttcttg ttacgatatg cggagtagta ggccgagatg
     7981 cctttccaga aggtggcgtc gccgagggtg cggcggagca tatgtagggc ccagccgcct
     8041 ttctcatagc tgttggggtt gagcagctgc atatagtccg agtggacgcc ggtgtcgacg
     8101 acgggggtga agcggcgttt ttcgaaggcg ataacctttg cgcggtcctt gcgcaggctg
     8161 gtccgcagcg tgtcgggacc atatttattt tccaggtaac agagggtcat ataggtggcg
     8221 aagccttcgc tgagccagag atgtcgccag gccgtctcgg tggcggcgtc gccgaaccat
     8281 tggtgtgcga tctcgtgggc catgagagat tcgacgccgg gcgagccgac cgagccctcg
     8341 taataaaaga tggcactcgc attctcgagt ccgccaaaac gggtttttga ttggacattg
     8401 ccttcttttt cgaaggggta gggcccgatg tgcgtgatat aaaagggcag gatatcgagc
     8461 gctacggcat agctgcggaa accggcttct ttattttgtc ggtagacata attccatacg
     8521 gggatgccca tggcgtcgcc aggatggtcg acggcgaaat ccgccacgcc gatgaccatg
     8581 atcttggggg cgatgggttc ggactctttc caacgggtga gcttgagatg gttggcgagg
     8641 ggcttttctt cctgcaatgc gccgttcgct acgactgtgt agtggtcggg tgatactatg
     8701 ctgaaatcga gggtggcctt gtccgaagga tcgtctacgc agggcagcca gttatgcgcg
     8761 cggttgggcc agttgtcgcc gaagaatccg cggcttccga atttattttt ggagatgatg
     8821 aggccatcgg cgggaatgcc ttcgtaatcg atgagatagg tgcgcgaact gccgggtgcg
     8881 cctcctgcat gcaggatgag ctggtcgtcc tgctgggtga agggaacggg tttgtcgcct
     8941 tcacggacgg aggtaacggt catgcctttg ccgtcggcat tttttccggt gaggtcgaga
     9001 tggaattcgt tggcgggctc acggaactcc agggtgatac ccgcgtcgcc tctgatgcgg
     9061 tcgctgctgt cattgagcca caactgaaag ttatagtgct gtacgtcgat ggccgggtta
     9121 tagggttggg cggtgagacg ggcaaagggt agaagagcga tgatggtcag ggtgcgaagc
     9181 aggagtttag gcattgggcg ttgatttttg cagggttggg aagggttaga cttttgcaag
     9241 atcgaaggga agccggcgga tacgcttgcc cgtggcggcg aaaactgcgt tggcgagcgc
     9301 gggggcgacg gggggaagac ctggttctcc cacgcctttc atgacgggtc ctccttcggc
     9361 gaggatatgt acttcgacgg ccggcatttc gttgattcgc agcatccggt tgttatgaaa
     9421 attggtttga acggatttgc caccctcgaa acggatgccg tcttttatcg cagcgacgag
     9481 acccatgacg atggcgccct cgacctgtgc acggaccgta tcgggattga cgacggtgcc
     9541 gagatcgatg accgagcaga ccttgtccac tttcaggctg ccgtcttttt ttgtcgacac
     9601 ttcgatgaca ttggcgcaga gaccggcgaa gaatttgtat tgggctacgc cccgtccccg
     9661 tccttttggc agcggtttgt cccaatgcga gacctcgcgg agatgtgtca ggacgcgccg
     9721 ggtatccgac tcttttgtga gcaggctcag acggaagtcc atcgggtcct tacccgccgc
     9781 ggccgccatc tcgtcgataa agcattcgtg tgcgaaggcc agcgtgctgc tggtgacgga
     9841 gcgccagggt tgcatcggga catggatatc cgcgaagacg taggcgttct tcatattcgg
     9901 aaattcgtag tcctgatcgc tgatgccttc agtcatggag cgatcgggtt tggtcttatc
     9961 gtatttgtcg gatcgtgtcg cgctgatcga aggggagatg accttgtgct ggaaagcgac
    10021 ggcttttcct tcggccgata cggcgccttt catggccgag aaggtcatgg gacggaacgg
    10081 tcccaattgt atatcgtctt cgcgtgtcca tagaaccttt acgggtttgc cgatggcttt
    10141 cgaaatgccc gcggcttctg cggcatagtc ggccgcgaga cgacggccaa agcctccgcc
    10201 gacgaatgag atatgcgcgg tgatgttctc tgccgggatg cctaaggtct gtgacagctc
    10261 gcgcgtgacg atgccggcgc cctggaccga agtccagata tccactttgt ctccttcctg
    10321 ccagtgggcg agacaattca tcggctccat cgttgcatga gcaacaatcg gtgtctcgta
    10381 gagggcttcg agtttgtggg gcgcggaggc catggcgccg tcgaagtctc cgctgtcgtg
    10441 ggcgaggact ccttcggttt ttgcgagatc gcggaggtgt tgttcgtaat cgttggaatt
    10501 aaatttgtca aagccctggt agtcccattg gactttgagc gcgcggcggc ccttgagggc
    10561 ggcccaatag ttatcggcga tgacggcgat gccttcgtaa tggttcttgc cgaggacgcg
    10621 ttgggctttt accgtcgttg tgacgccctt gactttgagg gtggcgctgt cgtcatagct
    10681 gacgagcctg gcgccgaaga ccgggcaacg ttcgaccgag gcgtagacca tgccgggcag
    10741 ctcggtatcg atgccataga ctgccttgcc ggttactttt agagggatat ccatgcgggg
    10801 catggactta cccaatattt tgaaatcttt gggatctttc aggggtggat ttgtcgggac
    10861 ctcgagtttc gaagcggctg ccacgaggtc gccgtatccg aggaatctgc cggtgggctg
    10921 gtgcaggatc tttccgttat ccgccttgca ttcttcgatg gggacattcc attgtttgga
    10981 tgcggcggta cgcagcatct cgcgcgcggc ggcgcctacc ttacgcaact gggtatagct
    11041 gcctcttacc gaggagctgc cgccggcgga ttgaccggga atatcttttt gaccccagct
    11101 ggtgaggata gtggtccctt cgagggagac ttcgagttct tcggcgatga gggctgggat
    11161 agactggtac gtgccctggc ccatttcggg tttcggatta attagggtga tcctgcccgt
    11221 gggctcgatg atgatatagg gggtaatgtt ggcaatttcg ggcaggcctc cggcgccggt
    11281 gatgtcgggt agccccgcaa gcggcagggc ggcggcatgg agtttggcga tggcggcttc
    11341 cgcgtcgtta tataccgaga tgcccaggat cagtgcggca gcagaaaatc cggtgtgctt
    11401 gaggaactcg cggcgggcta ttttatgttc aggcatggat aagtggtttt tgcagccttg
    11461 aaggagctgg ttttgcggcg gcggcggcca ggtggatggc cttgcggatg cggtcgtagc
    11521 acccgcagcg gcagatgttg ccctgcatgg cgatgtcgat gtctgcgtcg gtgggtttcg
    11581 gattgtgccg aagcagcgcg actgcgctca tgatctgacc cgactggcag tagccgcatt
    11641 gcggtacgtt ctcgtcgatc caggctttct ggaccgattc tcccactttg tcgccggtaa
    11701 ggccctcgat ggtagtgatc ttctggcctt cggcggcgga gatggggatg ccgcatgatc
    11761 ttacggcgct gctcccgaga tgtacggtgc aagcaccaca ttgggcgatg ccacacccga
    11821 atttggtgcc ctttagcccg atgagatcgc ggatgaccca tagaaggggc atgtcgggtt
    11881 cggcatccac ctgcacgggt ttattattga tggtaagcga ataaggcatg ggatgaaatt
    11941 tgatatgaat ttaccaataa ttcaggaaac tgattaattt gctatatgcc agaatatctg
    12001 aattgcccgt cgtggttcca cagattgccg tctatcgcca agctggcagc cagcgtcgtc
    12061 gtagcgggta tcatatccgc ctgtctgctg cctttc
//
LOCUS       DASYSV010000247         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1966298, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000247 DASYSV010000000
VERSION     DASYSV010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966298"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VGQ51_07625"
     CDS             <1..313
                     /locus_tag="VGQ51_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036471.1"
                     /translation="KLRDDAFKMTFKHSVKASSIDQTVIQTPNGVGGIFFHVGGNAAT
                     ANQFFLTDTTRHFLRGALYFDTTPNEDSLGIVNSFLEKDMEHLINTFRWKVPAAPAPR
                     K"
     gene            335..922
                     /locus_tag="VGQ51_07630"
     CDS             335..922
                     /locus_tag="VGQ51_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HEV9036472.1"
                     /translation="MIAIDNILVSDEVVREQFVCDLLKCKGGCCEDGDAGAPLEKEEL
                     DRLNEVYEVVKPYLTTAGVREIESGGRYQYDREFGWVTPTIGGKMCAYGIRDNNGIIK
                     CGIEQAFYDGKIGWKKPVSCHLYPIKTGKTKAGEVVNYEPRDTLCRPGCVQGKRLKTP
                     VYVFLKEALIRKFGKEFYSALEQAAEAHFSEKIKP"
     gene            919..>1715
                     /locus_tag="VGQ51_07635"
     CDS             919..>1715
                     /locus_tag="VGQ51_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEV9036473.1"
                     /translation="MTDTAANFIARSTVKAEDLDHRNKINFNIGRYNAAVPQGKQQFA
                     DLNLARERAKNIKWRAIENLDKQLEEFEAIFSQRGGRVIWAENTTQALAEILAICKEK
                     KCRSVVKSKSMVTEEIKLNHFLETNGIESVETDLGEYIQQLDGEPPYHIVTPAMHKSK
                     EDVAKLFYEKLNTRPGLTPEQLTLVARDRLRKKYIQAEIGITGANFIISDIGGVAVTE
                     NEGNARLSSAFPKTHIVIAGIEKVIPSMTDLGLFWPLLATYGTGQRVT"
BASE COUNT          459 a          452 c          448 g          356 t
ORIGIN      
        1 caagctgagg gacgacgcct ttaaaatgac cttcaagcac agcgtcaagg ccagttccat
       61 cgatcagacc gtcattcaga cccccaacgg cgtcggcggc atctttttcc atgtcggggg
      121 caacgctgcg actgccaatc aattcttcct taccgatact acgcgtcatt tcctgcgcgg
      181 cgccctgtat ttcgatacca cccctaatga ggattcgctg ggcatcgtca actcttttct
      241 cgaaaaggat atggaacatc tgatcaatac cttccggtgg aaagttcctg ccgctccggc
      301 accccggaaa tgacctatat ttgtaaccga cattatgata gccatagata atatcctggt
      361 aagtgacgaa gttgtccgcg agcagtttgt ctgcgatctc ctgaaatgta aaggcggctg
      421 ctgtgaggat ggggacgccg gcgccccgct cgaaaaggag gaactcgatc gtctcaatga
      481 ggtttacgaa gttgtaaagc cctacctgac aactgccggc gtccgggaaa tagaatccgg
      541 gggccgttat cagtatgata gagaattcgg ctgggtaacg ccgaccatcg gtggaaagat
      601 gtgcgcttat ggtatccggg ataataacgg gatcatcaaa tgcggcatcg agcaagcctt
      661 ttacgacggg aagatcggtt ggaaaaagcc agtcagctgt cacctttatc ccatcaagac
      721 cgggaaaacg aaagcaggcg aagtagtgaa ttatgaaccc cgcgacaccc tttgccgtcc
      781 cggctgcgta cagggaaaaa gattgaagac acccgtatat gtcttcctga aggaagcgct
      841 catccgcaaa ttcgggaagg aattctactc cgcgctcgag caggcggccg aagcacattt
      901 ttcagaaaag ataaaaccat gacagatacc gccgctaatt tcatcgcccg cagcacggta
      961 aaagcggagg acctggacca ccgcaacaag atcaatttca atattggcag gtataatgcc
     1021 gcggtacccc agggcaaaca gcagttcgcc gatctgaacc ttgcgcggga aagggcgaag
     1081 aacatcaaat ggcgggccat cgaaaatctc gacaaacagc tggaagaatt tgaagccatt
     1141 ttctcgcaga ggggcggcag ggtcatctgg gcggagaaca ccacccaggc gctggccgaa
     1201 atactcgcca tctgtaagga aaagaaatgc cgttcagtcg tcaagagcaa gagcatggtc
     1261 accgaggaga tcaagctgaa ccacttcctg gagaccaacg gcatcgaaag cgtagagacc
     1321 gatctcggag aatatattca gcaattggac ggggagcctc cgtatcatat cgtgaccccc
     1381 gccatgcata agagcaagga ggacgtagcc aaacttttct acgagaagct gaatacgcgc
     1441 cccggtctta ctcctgaaca gctgaccctc gtggcaaggg atcggcttcg taaaaaatac
     1501 attcaggcgg agatcggtat taccggtgcc aattttatca tctcggacat cggaggtgtc
     1561 gccgttactg aaaatgaagg taacgcgcgt cttagctccg ccttccccaa gactcatatc
     1621 gtaattgccg gcatcgaaaa ggtgatccct tccatgaccg atctgggcct tttctggccc
     1681 ttactggcga cttatggtac gggtcagcgc gtcac
//
LOCUS       DASYSV010000248        16662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2091762, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000248 DASYSV010000000
VERSION     DASYSV010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 16662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16662
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2091762"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VGQ51_07640"
     CDS             <1..252
                     /locus_tag="VGQ51_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9036474.1"
                     /translation="IEKLSRISAMSPTSLKTKFKRYYGDTLFGYYQKNKLEHARKLLD
                     NKVPVKVVATEIGYSNPSHFTLAFKKEYGFSPWHYLNPH"
     gene            311..1231
                     /locus_tag="VGQ51_07645"
     CDS             311..1231
                     /locus_tag="VGQ51_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9036475.1"
                     /translation="MKSQYPVLPKTARALSWQEFALPVGLRAAILKQSVEKMDLALPD
                     LPQNGITLTFLHYKGDVRVRWSGQAIEHAASPGSGLMIRSGRPVRFLSANPVTISALA
                     IFIPTGWADLFFDSNAHFRKMEQLMDEEENIITCRLGRRQLSMFATAFTGQDGNEPPL
                     LSLQNVVLTLLEYFLCNRHRSLLGVNPISDIDNEDDLSLIREVESYISEYYCSDTFTV
                     DSILKKTYTSYHRLNFLFKSIHGMTISEYIRNKRIEKSKEMIADNVKSISQIAYEIGY
                     SSISNYILAFKKVYQITPGKYKKQIDNLAG"
     gene            complement(1247..4339)
                     /locus_tag="VGQ51_07650"
     CDS             complement(1247..4339)
                     /locus_tag="VGQ51_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9036476.1"
                     /translation="MIVLTLSFKATPAQTPSLMGYTVQHFTDENGLPQNSINDLLFDQ
                     HGYLWLATQVGLVRFNGNSFRTYYPDDRPVMESNISYLGTDPGGHLFFQTTDRHLYLY
                     AGDQGGRLHPLNTPSTGKLLLNNRKQLFDFTAFLQQPQDPGQRLQRKAIFLDLFDHSD
                     NFFVSDTGHIYIRHHDSLSYYDGNRLFTLMPQVPAPSQYFIAEKRLYVLKGDSVLGVY
                     EKDRKIGGAEKIRGDGLHAATGPPAPEYRIFASGGHIHLLAGSKLYRLYPDSLRHIHS
                     AYLTDLSFIPLISAIEYNPGLDLLLVATLTEGFYFLRKNFFANPGWPDQLRKKMSGRL
                     FGPVALLQNEDILTDKFVFTPDGRSSTTKSPASGWRQCLFRDRAGNIWGDIGNRPVKM
                     KTDLTPVDIGPPLDADVMDYAQDPDGKMYCLTRQSLWSMDPRSGPDPLFNRLYTTDQL
                     PDKGDNESMAFIEPDRLWIGNTNGLIDFDIRKKDARPIQELSGMHVRAIHRCQDGSIL
                     IGTYGEGYFYYQHGHFYRMPLDKNGFLVTAHCFLEDHKGNIWIPCNKGLFRVPKADID
                     SWTKGETSLLFYYYYGRQDGLETNEFNGGFNPSGAITGKDFVALLSMKGIVCFYTDSL
                     PTDFPQDSIDLSEVEIDGKPVPVKDTIDLPAGYNSLNLEVSCPYLGNRNNLYLEYSLE
                     GLSDEWKQVPEDGMLNLSRLSPGNYTLLVRKVNGFGKNNFQYRQWSIIIPPTFYRSSG
                     FLFALGLALLILLVLLAQVSVKLIEKKREARENAETLKGTVTRLEETVSKLQLSEQAL
                     VKTSRQREKLISLVIHDLRSPLRFLTMLATDLHDHQSGLSATELNDRTWWIKKGAQDI
                     YHFSEDFLLWVTSQKDNFKLTKQLFLIRPLLREIHDFYLEQVLQKGNKIEYDADQNLQ
                     IYSDPHLLITIIRNLTDNANKYTDHGSIRITAEATEKGWLVTVSDTGKGMSPQQVEAF
                     LGEGSLDNVRSGSQLGHKFVYDLTQRLGGTLLVESKEGQGTTVSLLIPASSGQIATAL
                     "
     gene            4648..4965
                     /locus_tag="VGQ51_07655"
     CDS             4648..4965
                     /locus_tag="VGQ51_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEV9036477.1"
                     /translation="MQTFDITLNLFVFGSMLIVAVLLGYLRRSRQLAKKTRQIAKLEK
                     EMIQAHAELLETQREFCELEAKMKDITNPVIPMNSKSSDDQTQTPLPERDGMRHHRPA
                     GSR"
     gene            5241..6338
                     /locus_tag="VGQ51_07660"
     CDS             5241..6338
                     /locus_tag="VGQ51_07660"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEV9036478.1"
                     /translation="MRLLTNLFFKVTLSSMILTAPAMRTGNTDDGGKRNLLPQDQRTQ
                     TFYTSVVIRKFVDHPTVKAMRAVMRDESVTGASAASESAAGATTSVSAASVAATSAAP
                     GSVEETKAALANTAAISATTTSARETSAAATIAAGKVRELASVASGLTAMSAKAAEKR
                     AELRLVINQASLIYTSMKLDSSGLDEKAFEYAWRGYHNLLKKRMIRKRSVLSIVDFSQ
                     SSCSKRMYVIDVRHKRLLYRTYVAHGQNSGEEYASSFSNEPDSYKSSLGFYVTRWTYI
                     GHNGLSLKLAGVDSGYNDMAAKRNIVLHGSSYVGDRYMHEFGTLGTSLGCPAIPSAVS
                     GRIIRAVKGGSCLFIYHPTAQYLDNSSIING"
     gene            6404..7657
                     /locus_tag="VGQ51_07665"
     CDS             6404..7657
                     /locus_tag="VGQ51_07665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9036479.1"
                     /translation="MTPRQLQQAAALNHASLFRQEAATLGGTIIAREGLQWTSGSPTC
                     TSMIAFPELPPGQESHRLDEMMDFYLRHPPKGAGCWSLDPPQPADLGVRLLARGFQPG
                     WRPRWMALDLGQLQTHHPFPKGLSITIDNSSSLEAIQQLPYAQVIIPSLSLAERPVQW
                     TRFVARLQGRVVAHSVVFLSTGHYGAAGIYHVGVVPRARHIGIGKAVTVAACLHAREM
                     GYRYAVLNSTDAGRRTYEQLGFTTVGEGWTWWLITEKLLSRPPSPKEVQLAEAIGRGD
                     MDVLYSLPPSYMSGGGHHRLTNGMTLMELAVHCRQSDAAEWLVGSGAAFTVLDAWDLG
                     WKERARQRLLENPGTVNQLYGEWDKTLLHFAAERNDEELTRLALSASPDLRLRDKAYR
                     ATSLDWAKHFGHGVIADMIAGYRHV"
     gene            7789..10899
                     /locus_tag="VGQ51_07670"
     CDS             7789..10899
                     /locus_tag="VGQ51_07670"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036480.1"
                     /translation="MKKLAIPRLRWGLILFFALTGAFSQAQTPDTAFYGNLHWRMIGP
                     HRGGRTVGGCGVPQQPNVFYIGVNNGGVWKTTDFGRTWVSLFDAEPTGSVGDVAVAPS
                     DPNIVYVASGEGIQRPDLSVGDGIYRSDDAGKTWKHLGLAEGQQMGGLAIDPHDPNRV
                     FVAVLGHPYGPNPERGVFRTTDGGQHWEKVLYKDENTGAVQVTIDPVNPQVVYADLWA
                     GRQGPWENGSWNGPNSGLFKSTDGGTTWHQLTQGLPSTAQGLGRIGFCVAPSDPTRLY
                     ATVDAGANGGIYRSDDAGESWKKLNSDSRFWGRGDDFAEVKADPKNKDIVYTADVVVW
                     RSTDGGMSWNAYKGAPGGDDYHRIWINPDHPDILLIACDQGAIVTVNGGKTFSSWFNQ
                     PTAQFYHVSTDNAFPYNVYGGQQESGSVGIASRGNDGEITFREWHPVGVEEYGYIAAD
                     PLDPNIIYGGKITRYDKRTGQVQNIAPEVIRSGKYRFLRTAPVLFSPTDPHTLYFAGN
                     VLFKTVNGGHSWDIISPDLSRATWNIPASVGIYNTESLKKMPRRGVIYTVAPSPVDGA
                     TIWAGTDDGLIHVTHDGGKTWSNVTPPEITDWQKVSLIDAGHFDAHTAYAAVNGIRTD
                     DMHPHIYKTHDGGKTWTAITNGLPDGPINVVREDPHHRGLLLAGSEQCVYVSFDDGGH
                     WESLRLNMPATSIRDLVFKDADVVVGTHGRSFWILDDISPLRQLAGGKAVADAVLYKP
                     EPAIRIRWDMYTDTPLPGDEPAGENPPDGAIIDYYLKGDESTPVTLEILDGAGKQVRH
                     YSSTDTLYTVPDVNIPTYWIRPQQILSAAAGPHRFLWDLHYQPLNVPPSYPIGAVVGN
                     TAPSPTSPWVTPGVYTLKLTAGGKTYTQPLTVRMDPRVKTAVATLQQQHDLALAAYHG
                     RVRAMEAVEKIRALRTRIAASIPQASGDRLTALQQLDARAAALEGQGRRFGRGAAAPA
                     PFGPVSFSQLQGDYATIFTVLEEADLAPTVQAQSALQTTEQAARNTDAAWTALQQEAT
                     KLNIAP"
     gene            10896..14066
                     /locus_tag="VGQ51_07675"
     CDS             10896..14066
                     /locus_tag="VGQ51_07675"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036481.1"
                     /translation="MRLTILLLLAPGFLIAQRKSAPIAHSGNTDSSFFAPLKYRLIGP
                     FRGGRADAVVGSIGNKHTFYFGATGGGLWKTTDGGSNWKNVSDGWFGGGVGAVALAPS
                     DETIVYAGEGESTLRNNVSENQGGIWRSDNGGRTWHNLGLKDSRHIARIVVHPKNPDI
                     VWVAVEGHLFGPNTERGVYKTTDGGKTWKRVLYINDQTACSDLVMEPGNPSVLYAGMW
                     HVIRTPYSMESGGEGSGMYKSTDGGETWVNISGRKGLPKGIWGVVNISVAATNPDKVY
                     AMIENAHGGLFLSTDSGANWKLINSENEIKQRAWYFNRVFVDPTNENTIYCTNVEFMR
                     SLDGGKTFTRVQSPHSDHHDLWIDPQDGHRLILADDGGAQISYDFCENWSSLDNQPTA
                     QIYRVTTDNAFPYHILGAQQDNSSVRIKSRTTGPGITQRDWEPTAGFEAGYIVPDPLN
                     PDVVYGGNYDGYLGRLDHRTGESRAIDVWPDNPTGYGADSLKFRFQWNYPIFFSPHDP
                     HRLYCAGNQLFMTENEGKSWTAISPDLTTNDKSKQGASGGPITKDNTSAEYYCTIFTA
                     TESPLEKDLLWAGSDDGLIHVSRDGGGQWDLVTPPDAGKWTLWNCIETDPFRKGTAYA
                     VGMRFRLDDYTPYIYRTEDYGKTWKLITKGIAPMHFARVVRADHKRPGLLYAGTEYGM
                     YISYDDGDNWEPFQLNLPIVPVTDLTIKNNDLIIATQGRAFWSLDDLGLLQQKGDGIL
                     DQRLHVFEPGDAYRMPGGGRGRRREGGEALRNVGQNPPNGVVFRYWLKDAPDSPRVSV
                     TIFGKKHETIRTFSTRPRHDDPDSRLECQSGMNTFVWDMLYPPSVRIPQMVLWTGGAG
                     TPKSAPGKYTARFRYGKDSTDVAFTILADPNDSMSEADYDAQVAFLLQVQGKYNEVQK
                     AILRIRDLRTQLHDLNGRLDSAGRTIRKFSDSLSRELTSIEEALDQTKAKSSEDVLNY
                     PIRINDKLSGLYGVAAGGNAPPSAQVRDVYAELSVQADKQLHRLKEVENTGLPAVNKL
                     IYERQVPVISAKNEPSGTATHEENGN"
     gene            14056..>16662
                     /locus_tag="VGQ51_07680"
     CDS             14056..>16662
                     /locus_tag="VGQ51_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV9036482.1"
                     /translation="METNWLDISSTVGLIATAVLTFNLLLGMLLSTAYRRSVLWKKMP
                     GVVQRISIDDLHNWTAYVALALALAHPLLLLPDASLKYSVADIVAPVSAPHQPFWTVL
                     GSLAAYAVIVVVITTQKAIKRRMGFRAWKNVHLISYGTALLFIIHGIVMDPELKDRPV
                     DWLDGEKLISEICGLILIVATVIRYRWHLRNRRPKAVSAIVALLMLIGLQAFPQRYDS
                     SLFRQLRFRFIGPDGNRAIALAGVPGDRNVAYVGAASGGIFKTEDAGITWHPVFDSAD
                     NSSVGALAVAPSNPKQVWAGTGETFLIRPAEAMGNGVYKSVNAGRSWTNMGLQATGRI
                     GRVIVDPNDSNIVYVAALGNTHAPQQERGVYKTIDGGKSWERVLFVDSLTGCADLAID
                     PQHPGTLYAAMWQISFNTWMLKSGGPGSGIFKTTDGGRTWTRLGGGLPGGPGHPVGKT
                     SVDLAYSNPNIVYALIEDKDPALYRSDDAGVTWKKMFTSHSLAQRASYYTRVRVSTGN
                     PDDVFTMCVTVMESKNGGKSFNGVGENGDYKPGGDTHDMWFDSKDPLRAMVAHDGCLN
                     MTFTGGKSWENVNLPIAQMYHVAVDNQVPYYVYGNRQDGYSYRGPSNSLEGGIPTGLW
                     TYVGGCESGFAQPDPEDNNIIWSGCYDGGLDRTDLRTGDVRDVRAWPEAGYGYPPSEM
                     RYRWHWNFPMAVSRYVHDKVYIGSQYVHVTTDGGQTWKVISPDLTTNDKSHQQSSGGV
                     STDNLFTFDGCTLYVIAESPVKAGVLWTGSNDGLVHVTQDDGAHWEDVTANIPGLPKW
                     STIRAIDPSPFDAGTAYISADGQFIGDFNPHLYKTNDYGKTWTLISGTFPRSNSSFVH
                     QIIEDPGKKGLLW"
BASE COUNT         3819 a         4383 c         4776 g         3684 t
ORIGIN      
        1 atcgagaaat tgtcccggat atcggcgatg agcccgacca gtctcaagac caagtttaaa
       61 agatattatg gtgatacact atttggatat taccagaaga ataaattgga gcatgccagg
      121 aagttgttgg ataataaggt gccggtaaaa gtggtggcga cggagatcgg ttattccaat
      181 ccatcgcatt ttactttggc gttcaagaaa gagtacgggt tttctccctg gcattatctg
      241 aatccgcatt gatcctcgcc gctgagcaga acggaccgaa tttgccctta actcgaacca
      301 aaatatgact atgaaatccc aatatcctgt cctcccaaag accgctcggg ctttgtcatg
      361 gcaggagttt gcattgccgg tgggtctccg ggctgcaatt ttgaagcaat cggtcgaaaa
      421 gatggatctg gccctgcccg atcttcctca aaatgggatc acgctaactt ttctacacta
      481 taaaggcgat gtccgggtaa gatggagtgg gcaggccatc gagcatgccg ccagtccagg
      541 gtccggtctg atgataagat cgggaagacc agtccgtttt ctttctgcca atcctgtcac
      601 gatatcggcg cttgccatct ttattcccac cggttgggcg gatctttttt ttgatagcaa
      661 cgcacatttc cggaagatgg aacagctgat ggacgaggaa gagaatatca ttacctgtcg
      721 cctggggcgc cggcaactct caatgtttgc aacggcattc acaggacagg acggcaacga
      781 acctccgctg ctttcgctgc aaaacgtcgt gttgaccctg ctcgaatatt tcctttgcaa
      841 tcgtcatcgg agcctgctgg gcgtcaaccc catatcggac atcgacaacg aggacgacct
      901 gagtctgatc cgggaagtgg agtcctatat ctcggagtat tattgttccg atacgttcac
      961 agtggattcc atcctgaaaa agacctatac cagctatcac cggttgaatt tccttttcaa
     1021 atcgatccac ggcatgacga tctcggaata tatccgcaat aagcggatcg agaagtcaaa
     1081 agagatgatc gccgacaacg tcaaatcgat cagccagatc gcgtatgaga tcggctattc
     1141 cagcatcagc aattatatat tggcgttcaa gaaagtctat cagataacgc ccggaaagta
     1201 caagaagcag atcgataatc tggcaggctg agttgagatg cctggctcag agagcggtcg
     1261 cgatctggcc ggatgaggct ggaattagca ggcttactgt cgttccttgt ccttccttac
     1321 tctccaccaa aagagtcccg ccgagccgtt gggtaagatc gtagacgaac ttatgtccca
     1381 gctggctgcc gctcctgaca ttgtcgagac ttccctcacc caggaatgct tccacctgtt
     1441 gaggactcat gcctttgccc gtgtccgaga cggtgaccag ccatcctttc tccgttgctt
     1501 ctgccgtgat ccggatggat ccgtgatcgg tgtatttatt ggcattgtcg gtgaggttgc
     1561 ggatgatcgt gatcagcagg tgaggatcgg aatagatctg caggttctgg tcggcatcat
     1621 attcgatctt attgcctttt tgtaacacct gctcgaggta gaaatcatgg atctcgcgta
     1681 acagcggccg gatgaggaac agttgtttgg tcagtttgaa attgtctttc tggctggtga
     1741 cccaaagcag gaaatcttcg gagaaatgat agatatcctg cgcgcctttt tttatccacc
     1801 aggtgcgatc gttgagctct gtggctgaca ggccggactg gtgatcgtgc agatcggtag
     1861 ccagcatggt aaggaatcga agcggagatc gcagatcgtg tatgaccagc gagatgagtt
     1921 tttcacgttg ccggctggtc tttaccaacg cctgttcgga gagctgcagt ttcgaaacgg
     1981 tttcttcgag acgggttacg gttcctttta gcgtttccgc gttctccctg gcctccctct
     2041 ttttttcgat gagcttcacg cttacctgtg ccagcaggac gagcaggatc aataatgcca
     2101 gcccgagcgc gaacaggaac ccgctcgacc ggtagaatgt cggtgggatg ataatgctcc
     2161 attggcggta ttgaaaattg ttctttccga agccattcac cttccttacc aagagggtat
     2221 agtttccggg tgagaggcgg ctcaggttga gcatgccgtc ctccggaacc tgtttccatt
     2281 catcactcag cccttccagg ctgtactcga gataaagatt attccgattg ccaagatagg
     2341 ggcaggacac ttccaggttg agactgttgt atcccgcggg aagatcgatg gtgtctttta
     2401 cggggacagg ctttccgtcg atctctacct cggacaagtc gatcgaatcc tgtgggaaat
     2461 ctgtaggcag tgaatcggta tagaagcaaa cgatgccctt catggatagc agggccacga
     2521 aatctttgcc ggtgatcgcg ccggagggat tgaaaccgcc attgaattcg ttggtctcca
     2581 gaccgtcctg gcgtccataa taataataga aaagcaggct tgtctcgcct ttggtccagc
     2641 tgtcgatatc ggccttgggt actctgaaga gtcctttgtt acaggggatc cagatatttc
     2701 ccttatggtc ttcgagaaag cagtgcgccg ttaccaggaa gccgttctta tccagcggca
     2761 tccggtaaaa atgtccgtgc tgataataga aatagccttc gccgtaagta ccgatcagga
     2821 tcgaaccatc ctgacagcga tggatcgccc tgacgtgcat cccggataat tcctgaatgg
     2881 ggcgggcatc ctttttacgg atatcgaaat cgatgagccc attggtattt ccgatccaca
     2941 acctgtccgg ttcgatgaaa gccatcgact cgttgtctcc tttgtcgggc aactgatcgg
     3001 tcgtatagag gcgattgaac agcggatcgg ggcccgatcg gggatccatg ctccatagcg
     3061 actggcgggt caggcaatac attttcccgt cggggtcctg cgcataatcc atgacatcgg
     3121 cgtccagcgg cgggccgata tctacggggg tcaggtccgt cttcattttc acaggccggt
     3181 tgccgatgtc accccagata ttgccggcgc ggtcccggaa cagacattgt ctccagcctg
     3241 aggcggggct ctttgtcgtt gaggatcggc catcgggtgt aaagacgaac ttgtcggtca
     3301 ggatatcctc gttctgtagc aacgccaccg gaccgaagag cctcccggac atttttttac
     3361 gcagctggtc cggccacccc gggttcgcga agaaattttt ccgaaggaag taaaagcctt
     3421 cggtcagggt ggctaccagc agcaggtcga gccccggatt gtactcgata gccgaaataa
     3481 ggggtatgaa agacaagtcg gtcaggtagg ccgagtgtat atgccggaga gagtctggat
     3541 aaagccggta tagcttgctg cctgcgagta aatgaatatg accgccggag gcaaaaatgc
     3601 gatattccgg ggccggtggg ccggtggctg cgtgcaagcc atcaccgcgt atcttttccg
     3661 ctccgccgat cttccggtct ttttcataga ccccgaggac cgaatcccct ttcagcacat
     3721 acaggcgttt ttctgcgatg aaatattgag aaggggccgg cacctgcggc atcagcgtaa
     3781 aaaggcggtt gccgtcataa taagagaggc tgtcgtggtg gcggatataa atgtgaccgg
     3841 tatccgaaac gaaaaagtta tcgctgtgat cgaaaaggtc caagaagatg gcttttctct
     3901 gcaatcgttg tcccgggtcc tgcggttgtt gcaggaatgc ggtgaaatcg aaaagctgtt
     3961 ttctgttatt gagtaatagt ttcccggtgg agggtgtgtt cagcggatgc agacggccgc
     4021 cctggtcgcc ggcataaagg taaaggtgcc gatcggtggt ttggaaaaac agatggcccc
     4081 cagggtctgt tcccaggtaa gaaatattgg attccatcac cggccggtcg tcagggtagt
     4141 aggtcctgaa cgaattcccg ttgaaacgga cgagaccgac ctgtgtcgcc agccacaggt
     4201 atccatgttg atcgaacaga aggtcgttaa tgctgttttg cggcagtccg ttctcatccg
     4261 taaaatgttg aacggtatag cccattaacg agggtgtctg tgcgggggta gctttaaaag
     4321 aaagggtaag aacgatcaaa aaccagataa acttgtttat catgccttaa ccttgagtgc
     4381 taaaattagc ggaaataagc agaggatgac acccggtgct ttcttttttt aaattatatt
     4441 ttaaattaat acgtaattcg atgtatgtat gatatcatac gtcattgtcc gggttcgcta
     4501 ttctttttca gcaggcgggt aaatgctggt tacgcggctg ggtactttta tacaagcaaa
     4561 atcagtattc tatgtataaa ttctacacgc gcagtggcaa aggatgaatc atttgtaaaa
     4621 ccccatttaa tcaccaaatg atcatctatg cagaccttcg atatcaccct caatttgttt
     4681 gtattcggat cgatgctgat cgtagcggtc cttcttgggt atctccggcg aagtcgtcag
     4741 ttggccaaga agacccggca gatcgccaag cttgaaaagg aaatgatcca ggcgcatgcc
     4801 gagttgctcg agacgcaacg ggagttctgt gaactggaag cgaagatgaa ggacattacg
     4861 aatcccgtga tccctatgaa tagtaaaagt tcagacgatc aaacgcaaac gcctctgcct
     4921 gaacgggatg gaatgcgcca tcaccgcccc gcagggtcgc gttgaatcat ctaaaccttg
     4981 tctttatgcc gtacccgatc acggccgtct ccggcggcct gccaaaacga cttttatccc
     5041 gattgaccgg cctgctgata gcggtcggcg ctaatgctct tgacccggct ctagattatt
     5101 ctgttcccgg cgtcccgccg ggtcatattt ccgcgattta tgcgttatat agtggggaca
     5161 cgcgcggaat cggataaggg gaaaaatagt tctcctgttc aatcgaaacc ctatgtctaa
     5221 tatccttaaa tcgaaatcca atgcgcctat taaccaacct tttttttaag gttaccttat
     5281 cgtccatgat cctgaccgcg cctgctatgc ggaccggcaa tacggatgat gggggaaaac
     5341 gtaacctgct accacaagat cagagaaccc aaacttttta tacttctgta gttatccgga
     5401 agttcgtcga tcatcccacg gtgaaggcga tgagggccgt gatgcgggat gaatccgtga
     5461 ccggtgcgag tgcggccagc gaaagcgcag ccggcgctac caccagtgta agtgctgcca
     5521 gcgtcgccgc gaccagtgcg gctccgggaa gcgtagaaga gaccaaagcc gccttggcga
     5581 atacggccgc gatcagtgca accacgacta gtgccaggga gaccagtgcg gccgcaacga
     5641 tagccgccgg gaaagtacgt gagctggcat ctgtcgcttc cgggctgaca gcgatgagtg
     5701 ctaaagcagc tgagaaaagg gcggagctgc ggctcgtgat caatcaggcc agcctgatct
     5761 atacgagcat gaagctagat agttccggtt tggatgagaa agccttcgaa tacgcctggc
     5821 gggggtatca caacctgttg aagaaaagga tgatccggaa gcgcagtgtc ctttcgatcg
     5881 tcgatttcag ccagtcttcc tgcagtaaaa gaatgtatgt catcgatgta cggcataaaa
     5941 ggctgctgta tcgaacctat gtcgcgcacg ggcagaactc cggtgaggag tatgccagca
     6001 gtttttcgaa cgagccggat tcctacaaga gcagtctggg gttttatgtg accaggtgga
     6061 cctatatcgg acacaatggt ctttcgttga agctggctgg agtggattca ggctataacg
     6121 acatggccgc caaaaggaat atcgttttac acggatccag ctatgtcggc gaccggtata
     6181 tgcatgaatt cggtacgctc ggaacctcgc tgggttgccc ggccattcct tcggcagtca
     6241 gcggccgcat tatccgcgca gtaaagggag gaagctgtct tttcatatat catccgacgg
     6301 ctcaatattt ggacaattca tcgatcatca acgggtgatc ttctttcaat aagttttaga
     6361 cgcgaggggc caaaaggccc ctcgcgtcgt atatttgggt acgatgactc cccgtcaatt
     6421 acagcaggct gcggcgctga atcatgcgag cttattcaga caggaggccg ccacgctcgg
     6481 cggcaccatc atcgcccggg aaggtcttca atggaccagt ggcagtccga cctgcacttc
     6541 catgatagcc ttccctgagc tgccgcccgg gcaggaaagc catcgattgg atgagatgat
     6601 ggatttctat cttcggcatc cgccgaaggg tgcgggttgc tggtcgctcg accctcccca
     6661 gcctgccgat ctcggggtcc gattgctggc gcgggggttt cagccgggat ggcgtccccg
     6721 ctggatggcg ctggatctcg gacagctaca aactcatcac ccgtttccaa aaggattgtc
     6781 catcaccatc gataacagca gctcgttgga agctatccaa caactgccat atgcacaggt
     6841 gatcatacct tctctctcgt tggccgagag acccgtgcaa tggacccgtt ttgtcgcgcg
     6901 gttgcagggg agggtcgtag ctcatagcgt cgtttttctg tcgaccggac attatggcgc
     6961 tgccgggatc tatcatgtcg gggtagtacc cagggcacgt catatcggta tcggaaaggc
     7021 cgtgacggtt gccgcctgtc ttcatgcacg tgagatgggt tatcggtatg ccgtgctgaa
     7081 ttctaccgat gcgggcagac gtacgtatga acagctgggt tttacaacgg taggagaagg
     7141 ctggacctgg tggctcatca cggaaaagct gctttcgcgc cctccctctc cgaaggaggt
     7201 gcaacttgcg gaagccatcg gtcgcggcga catggatgtg ttgtattcat tgcctccttc
     7261 atacatgtcc ggagggggcc atcaccggct gaccaatggg atgacattga tggaactggc
     7321 ggtgcattgt cgtcaatccg atgcagcgga atggctggtc ggcagcggtg cggcatttac
     7381 ggtgctcgac gcctgggacc tggggtggaa ggagagggcc agacaacggt tgctggaaaa
     7441 tccggggacg gtcaaccagc tatacggtga atgggacaaa acattgctgc attttgccgc
     7501 cgagcgtaat gacgaagagt tgacgcgtct tgcgctatct gcgtcgccgg atctgcggct
     7561 gcgtgataag gcctaccggg ccacgtcgct ggattgggcg aaacatttcg gacacggcgt
     7621 catcgcggat atgatcgcgg gctatcgtca cgtatagggg ggaccgctac cgatgaggga
     7681 catggcgacg acagtcgctc ctgatttcac cgataaaaaa aagttgatgg acggactagg
     7741 ccgaaaagga ttatgttata ccttttgccc gctttcaaca cactgcacat gaaaaaactg
     7801 gccatccccc gtctgcggtg gggtttaatc ctctttttcg cattgacggg agccttctcg
     7861 caggcacaaa caccagatac tgctttttac ggtaatctcc actggcgaat gatcggaccg
     7921 catcgtgggg gccgtactgt cggcggctgc ggtgtaccgc aacaacccaa tgtcttttac
     7981 atcggcgtca acaacggcgg agtatggaag acgaccgatt tcggaaggac ctgggtatcg
     8041 ttgttcgatg cggagccaac gggctccgta ggcgacgttg ccgtcgctcc ttcggacccc
     8101 aacatcgttt atgtcgccag cggcgaaggg atacagcgcc ccgacctctc ggtcggcgac
     8161 ggaatctacc gaagcgatga cgccggcaag acctggaagc atctcgggct ggcggaaggt
     8221 cagcagatgg gcggtcttgc catcgatccg catgatccca accgtgtctt tgtcgccgtg
     8281 ctgggtcatc cttacggacc caatcccgaa agaggcgttt tcaggaccac cgacggcggc
     8341 cagcactggg agaaagtatt gtacaaagat gaaaatacgg gcgccgtaca agtgaccatc
     8401 gacccagtga atccgcaggt ggtgtacgca gacctgtggg ccggaaggca aggtccctgg
     8461 gagaatgggt cctggaacgg acccaacagc ggtctgttca aatcgacgga tggaggcacc
     8521 acgtggcatc agctgacaca gggattgcct tccactgcac agggtctcgg ccgcatcggt
     8581 ttctgtgtcg cgccctccga tccgacgcgt ttgtatgcca ccgtggatgc cggcgccaat
     8641 ggcggtatct atcgcagcga tgacgcgggc gagtcctgga agaagctgaa cagtgattcg
     8701 cgtttctggg gccgtggtga cgattttgcc gaggtcaagg ccgatccgaa gaataaagac
     8761 atcgtttaca ctgcggacgt tgtggtgtgg agatccacgg acggcgggat gtcctggaat
     8821 gcatataagg gcgctcccgg cggcgatgac tatcaccgca tctggatcaa ccccgatcat
     8881 ccggacatcc tgctcatcgc ctgcgaccag ggcgccatcg tcacagtcaa tggaggcaag
     8941 accttttcgt cctggtttaa ccaacctacg gcccagttct atcacgtgag cacggacaat
     9001 gcttttccct acaatgttta tggcgggcaa caggaaagcg gttcggtggg catcgcctcc
     9061 cgtggcaatg acggggagat cacctttcgg gaatggcatc cggtaggagt ggaggaatac
     9121 gggtatatag cagccgatcc gttggatccc aatatcattt atggaggcaa gataacgcgc
     9181 tacgacaagc gaacaggaca ggtgcagaat attgcgcccg aggtgatacg cagcggaaaa
     9241 taccggtttc tgcggaccgc gccggtcctt ttctcgccta ccgatccgca tacgctgtat
     9301 ttcgcaggca atgtgctttt caagaccgtg aacggcgggc attcctggga tatcattagt
     9361 ccggacctca gtcgcgccac ctggaatatc ccggcgagtg tgggcattta caatacggag
     9421 tcattgaaga agatgccgcg gcgcggggtc atctatacgg tcgccccgtc tccggtggac
     9481 ggcgcaacta tctgggctgg tacggacgac ggactgatcc atgtcacgca cgatggcggc
     9541 aagacctggt cgaacgtaac gcccccggaa ataaccgatt ggcagaaggt atcgctgatc
     9601 gacgcggggc attttgacgc ccataccgcc tatgccgcgg tcaacggcat ccggacagac
     9661 gacatgcatc cgcatattta caaaacccat gacgggggaa agacatggac ggccatcacg
     9721 aatggtctcc ccgacggccc gatcaatgtc gtgcgcgagg acccgcatca caggggatta
     9781 ctgctggccg gatcggaaca gtgcgtatat gtctctttcg atgacggcgg acactgggag
     9841 tccctccggt tgaatatgcc ggctacctcc atacgcgatc tcgtctttaa agatgcggat
     9901 gtggtcgtgg gtacccatgg ccggtcgttt tggatactgg atgatatttc gccgctgcgt
     9961 cagctggcag gtgggaaggc tgtggccgat gcagtcttat ataaacccga gccggccatt
    10021 cgtatccgct gggatatgta caccgacacg cctttaccgg gcgacgagcc cgcgggcgag
    10081 aatccacccg acggtgcgat catcgactat tacctgaagg gcgacgagtc gacccctgtg
    10141 acgctcgaaa tcctagatgg tgccggaaaa caggtgcgtc attattccag cacggacacg
    10201 ctttatactg tcccggacgt caacatccct acctattgga tcaggccgca gcaaatattg
    10261 tctgcggcag cgggcccgca tcgttttctg tgggatctcc attatcagcc gttgaatgtt
    10321 ccgccatcct atcccatcgg cgccgtcgtt gggaataccg cgccgtctcc gacatcgccc
    10381 tgggtcactc ccggcgtcta cactctgaag ctgacagccg gaggaaagac gtatacgcag
    10441 ccgttgacag tccggatgga ccctcgggtc aaaacagccg tcgcgacgct gcaacagcaa
    10501 catgacctgg cgctggccgc ctatcacggg cgcgtcaggg ccatggaggc cgtcgaaaag
    10561 atcagggcat tgaggacccg gattgcggcg tccataccgc aggcatcagg cgaccggctg
    10621 acagcacttc aacagctgga tgccagggct gccgcactgg aaggacaggg taggcgcttt
    10681 ggccggggag cggccgcacc cgctcctttc ggacctgttt ccttttcaca gctgcaggga
    10741 gactatgcca ccatctttac ggtcctcgaa gaagccgatc tggctcctac cgtacaggct
    10801 caatcggcat tacaaaccac ggagcaggcc gcccgtaata ccgatgccgc ctggactgcc
    10861 ctccaacagg aagctactaa actaaatatc gcaccatgag actaaccatc cttttactgc
    10921 tcgcaccggg ctttctgatc gcgcagagga aatccgcgcc cattgcgcat tcgggcaaca
    10981 ccgattcttc cttttttgcc ccgctgaaat accggctgat cggtcccttc cgcggcggca
    11041 gggccgacgc ggtcgtcggc agcatcggca ataaacatac tttctatttc ggcgctacgg
    11101 gcggcggtct ctggaagacc accgatggcg gcagcaactg gaagaatgta tcggacggct
    11161 ggttcggcgg cggggtagga gccgtggcgc tggcgccttc cgatgaaacc atcgtatatg
    11221 cgggagaggg agaaagcact cttcggaaca atgtttccga gaaccagggc ggcatctggc
    11281 gcagtgacaa cggcggacgc acctggcata acctgggatt gaaagacagc cgtcatatcg
    11341 cacgcatcgt cgtacatccc aaaaatcccg acatcgtatg ggtcgctgtg gagggccatc
    11401 ttttcggacc caacaccgaa cggggcgtct ataagaccac cgacggaggc aagacctgga
    11461 agcgcgttct ctatatcaat gaccaaaccg catgttctga tctcgtcatg gagccgggta
    11521 acccttccgt tctatatgcc ggtatgtggc atgtcataag gacaccctac agcatggaga
    11581 gcggtggcga aggcagcggt atgtataaga gcaccgacgg cggggagacc tgggtgaata
    11641 tttccgggcg gaagggattg ccaaaaggca tctggggagt ggtgaatata tcggtggcgg
    11701 ccaccaaccc cgacaaggtg tatgcgatga tcgaaaatgc gcatgggggt ctttttctga
    11761 gcaccgacag cggcgcgaac tggaagctga tcaatagtga aaacgagatc aaacaacgcg
    11821 cgtggtattt caacagggta tttgtagacc ccaccaacga gaataccatc tattgtacga
    11881 atgtcgaatt tatgcgtagc ctcgacggcg ggaaaacatt tacccgcgtt cagtcgcccc
    11941 acagcgatca tcacgatctt tggatcgatc cgcaggacgg gcatcgtctt atcctcgcag
    12001 atgacggcgg cgcgcagatc agctatgatt tctgcgagaa ctggagctcg ctggacaacc
    12061 agcccacggc ccagatctac cgggtaacga ccgacaatgc cttcccttat catattctgg
    12121 gcgcgcagca ggacaattcg agcgtgcgta tcaagagcag gacgaccggg ccgggcatta
    12181 cccaacgcga ctgggagccc acggcaggtt tcgaagccgg ctatatcgtg cctgacccgc
    12241 tgaaccccga tgtggtatac ggcggtaatt acgatggtta tctcggccgc ctcgatcatc
    12301 gcaccgggga gagcagggcc atcgacgtct ggcccgataa cccgaccggt tatggagccg
    12361 actcgctgaa attccggttc caatggaact accctatctt tttttctccg catgatccac
    12421 atcgtctcta ttgcgccggc aaccagctgt tcatgaccga aaacgaaggg aagagctgga
    12481 cggcgatcag ccccgatctg accacgaacg ataaatcgaa acaaggcgcc agcgggggac
    12541 ccatcaccaa ggacaataca tccgcggaat attactgcac gatcttcacg gctaccgagt
    12601 cgccgctgga aaaagacctg ttatgggcag gaagtgatga tggcctgatc catgtcagcc
    12661 gcgacggcgg tggtcaatgg gacctcgtaa cgccacccga tgccggtaaa tggaccttat
    12721 ggaattgcat tgagaccgat cctttcagga agggaaccgc gtacgcggta ggcatgcgtt
    12781 tccggctgga tgattataca ccttatatct accggacaga ggactacggc aagacctgga
    12841 agctgatcac caaagggatc gcacccatgc attttgcacg tgtcgtccgg gcagatcaca
    12901 aacgcccggg tcttttgtat gcgggaaccg aatacggcat gtatatcagt tatgacgatg
    12961 gcgacaattg ggagcctttc cagctgaacc tgccgatagt acccgtaacc gatctcacta
    13021 taaaaaataa cgatcttatc atagcgacgc agggcagggc cttctggtca ctcgacgatc
    13081 tgggtctgct acagcaaaag ggcgacggta tcctggatca gcgtcttcat gttttcgagc
    13141 cgggcgatgc ctatcggatg cctggtggcg gaagaggacg gagaagagaa gggggagagg
    13201 cccttcgcaa tgtgggtcag aacccgccca atggggtcgt attccggtat tggctaaaag
    13261 atgctccgga ctcgccgaga gtgagcgtca ccatctttgg caagaagcac gaaacgatcc
    13321 gcacgttcag cacaaggcca agacacgacg acccggactc acggctggaa tgtcagtcgg
    13381 gcatgaatac cttcgtatgg gatatgctgt atccaccgtc cgtgcgcatc ccacaaatgg
    13441 tgctgtggac gggaggagcg gggactccaa agtccgcacc gggaaagtat acggcgcggt
    13501 tccgttatgg caaggattcg acggacgtgg cctttacgat cctcgcagac cccaatgact
    13561 cgatgagcga ggcggactat gatgcgcagg tggccttcct gctacaggtg cagggcaagt
    13621 acaatgaagt gcagaaggcg atcctccgta tccgggacct gcgaacacaa ttgcacgatc
    13681 tcaacggccg gctcgacagt gcgggacgaa ctatcaggaa attttcggat agccttagcc
    13741 gtgagctgac ctcgatagag gaagcgctgg accagacgaa ggcgaagagt tcggaagacg
    13801 tactcaacta tcccatccgg atcaacgata aattgtccgg tctttatggc gtcgccgcgg
    13861 gcgggaacgc cccgcccagc gcgcaggtgc gggatgtgta tgcagagctg agcgtgcagg
    13921 cagacaaaca gctacaccgg ctgaaagaag tggagaatac gggactgcct gcagtcaaca
    13981 aattgatcta cgaacggcag gtgccggtga tcagcgcaaa aaatgagccg tcagggacgg
    14041 ccactcacga agaaaatgga aactaactgg ctggatattt cttcgacggt cggactcatc
    14101 gctaccgctg tcctgacctt caacctgctg ctgggcatgc tgctgagcac cgcctaccgg
    14161 cggtcggtcc tttggaagaa gatgccgggg gtcgtgcaga ggatcagcat cgacgatctg
    14221 cataactgga ccgcgtatgt ggcgcttgca ctggcgttgg cgcatccgct gctattgctg
    14281 ccggatgcgt ctttgaaata ctccgtcgca gatatcgtcg ctcctgtctc ggcaccgcat
    14341 cagcccttct ggacggtgct cgggtccctg gcagcatatg ccgtcatcgt tgtggtgatc
    14401 acaacgcaaa aggcgatcaa acggcggatg ggtttccgcg cctggaagaa cgtccatttg
    14461 atctcttatg gtacggcatt gctcttcatc atccacggga tcgtaatgga ccccgaattg
    14521 aaagacaggc ccgttgactg gctggacggg gagaagctga tctcggagat ctgcgggctg
    14581 atcctgatcg tcgcaacggt gatccggtac cgttggcatc tccggaatcg tcggccgaaa
    14641 gcggtcagcg cgatcgtggc attgctgatg ctcatcgggc tgcaggcctt tccgcagcgc
    14701 tatgacagta gtcttttccg gcaattgcgt ttccgtttca tcggtcccga tgggaacagg
    14761 gctatcgcgc ttgcgggcgt gccgggcgac aggaatgtag catatgtcgg cgccgcctcg
    14821 ggcggtatat tcaaaacaga ggatgccggg atcacctggc atccggtctt cgacagtgcg
    14881 gacaattcct ccgtgggcgc gctggccgtt gcgccatcca atcccaaaca ggtgtgggcg
    14941 ggtacgggtg agacctttct catccggcct gcggaagcca tgggaaatgg ggtatataag
    15001 tcggtcaacg ccggacggag ctggaccaat atgggtttgc aggccacggg acgtatagga
    15061 cgtgtgatcg tcgatccgaa tgattcgaac atcgtgtatg tggctgctct cggtaataca
    15121 catgcgccgc agcaagagcg gggcgtttat aaaaccatcg acggcggcaa gagctgggaa
    15181 cgggtgctat ttgtcgattc attgacgggg tgcgccgatc tggcgatcga tcctcagcat
    15241 cccggcacgc tgtatgcggc tatgtggcag atcagtttca acacctggat gcttaaaagc
    15301 ggcggtcccg gtagcggtat attcaagacc accgatggcg gaaggacctg gactcggctt
    15361 ggcggcggtc tgcccggagg ccctggacat cctgtcggca agacctccgt ggatctagcc
    15421 tacagcaatc caaacatcgt ctatgcattg atcgaagaca aagacccggc gctttatcgc
    15481 tcggacgacg cgggcgtcac ctggaaaaaa atgttcacta gtcatagcct ggcgcagcgg
    15541 gccagctatt atactcgggt acgggtctcc acggggaacc cggacgacgt gttcacgatg
    15601 tgcgtgacgg tgatggaatc caaaaatggc ggtaaatcct ttaatggagt gggggagaac
    15661 ggcgattaca aaccgggagg cgatacgcac gatatgtggt tcgattccaa agacccgcta
    15721 cgtgcaatgg tcgcgcatga cggctgtttg aacatgacct ttaccggtgg aaagagctgg
    15781 gagaacgtga accttcccat cgcccagatg tatcatgtgg ccgtcgacaa ccaggtccct
    15841 tattatgtat atggcaaccg gcaggacggc tattcctatc gcgggcccag caacagcctg
    15901 gaaggcggta tccccaccgg attatggacc tatgtcggcg gttgcgaaag cggtttcgcg
    15961 cagcccgatc cggaagacaa caatatcatt tggtccggct gttatgacgg cggtctcgac
    16021 cgaaccgatc tgcgtacggg cgatgtccgc gacgtacggg cctggcctga agccggatat
    16081 ggctatccgc cctcggagat gcgctatcgc tggcactgga atttccctat ggccgtttcg
    16141 cggtatgtgc atgacaaggt atacatcggc agccagtacg tgcatgttac taccgacggc
    16201 ggtcagacct ggaaggtcat cagtccggat ctgacgacga atgacaaatc gcatcagcaa
    16261 agttccggcg gcgtcagcac cgataatctt tttaccttcg acggctgcac cttatacgtg
    16321 atcgcggaat ctccggtgaa agcaggggtg ctctggacgg gcagcaatga cggactggta
    16381 catgttacac aggatgacgg agctcattgg gaagatgtga cagccaatat acccggtctg
    16441 cccaaatggt ccacgatccg tgccatcgat ccttcgccat tcgacgcggg gacggcctat
    16501 atctccgccg acggtcagtt catcggtgat ttcaatcccc acctttacaa gacgaatgat
    16561 tacggtaaga cctggacgct tatcagcgga acttttccca ggagcaactc ttctttcgtg
    16621 caccagatca tcgaagatcc gggtaagaaa ggtctgctct gg
//
LOCUS       DASYSV010000249         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_171061, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000249 DASYSV010000000
VERSION     DASYSV010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171061"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2122
                     /locus_tag="VGQ51_07685"
     CDS             <1..2122
                     /locus_tag="VGQ51_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036483.1"
                     /translation="MELERKNRPGAINDLRLCIRYSAGSFNNQKNKAFLQLGNLSFDT
                     KDYRSAKSFYDSLNLSASDMASTGDISWLSDRKAALTIIVANLEVIDRQDSLQRIAAL
                     PPAQRDAYIKRLVRTLRRQQGLRDEQDTSFSVSRLNSNNTVPDLFAGNGAEWYFDNQT
                     ARAKGFSDFRTNWGNRPNVDNWAIASIMRTNMPTAPGARRTIDMADSAGRMAASTRID
                     YKSLLGHLPLTPDKMQKSMDSIEHALFGLGRSYQDGIADYPYAIGAYDSLLYHFPATK
                     HREESLFNLYYCYRQLGDQPHADSVLRLLSEKYPKSNFTARILNPDSVEQAQTSQKLN
                     ATRQYDKVYVDFIEGRFDDALAEKKIADSLYGDKYWTPQLLYIESVYFIKTRQDEKAR
                     AILMSIMNRFPKTPMADKAGSLLDVLGRRRQIEDYLTRLKVTRAKDEDTVLNAAPDTA
                     GQAAANRRPRLIRNDSNMLVKEDTSSWARGKAREQALANPAGAPPKPNAIGPAKMNVD
                     TGSIRKISMDASQLASLHHLQDSLNRAMQKAQSDSAQMARLRQRSDSINNALKKLQSD
                     TAQLAAKLRTMNSVFTMTPDKPHAVIIVLDRVDPVYVSEAKNAFSRFNLETYYSKGLT
                     TENATLSDSLKLVVINGFPNAGDALGYLKTVKPEAPRDIVPWLPAGKYSFLIISNNNL
                     QLLLDNKDMPAYRKFLSAAYPTTF"
     gene            2212..>3362
                     /locus_tag="VGQ51_07690"
     CDS             2212..>3362
                     /locus_tag="VGQ51_07690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV9036484.1"
                     /translation="MSSNANRIFWRAFFYGLGGFILFLVLINLGVFGALPSLKELENP
                     SITLATEVFAEDGTPMGKYYKDKGNRSNVEFKDISPNVVNALIATEDERFYEHSGIDG
                     YSVMRAVLKMGRDGGGSTITQQLAKNLLEQGSKNMVRRFIEKLKEWIIAIKLERNFTK
                     QEILALYLNEVPFGDNVYGIRNAARTFFQKEPDRVTVDEAAILIGMLKGNTLYNPRRN
                     PKMAIDRRNTVINQMVKNNYLTEADAARFKARPIDLSNYKKIDENNGLSPYFRDVIRG
                     ELQRWCKEHKNPATGEPYNLYSDGLKIYTTINPRMQIYADEAVAKQLPILQKALSAQY
                     DVRKGLIWKDHANILDAAMHNSDRWHNLEDDGLTDADIRKTFYQPVAMKV"
BASE COUNT          891 a         1062 c          779 g          630 t
ORIGIN      
        1 gatggagctc gaacgcaaga accgtccggg cgccatcaat gaccttcgcc tttgtatcag
       61 gtacagcgcc ggctccttca acaatcagaa gaacaaagcc ttcctccagc tcggcaacct
      121 ctccttcgac acaaaagact accggtccgc aaaaagcttc tacgacagcc tcaatctcag
      181 cgcctccgat atggcctcga caggagatat cagctggctc agcgaccgga aggccgccct
      241 gaccatcatc gtcgcaaacc tcgaggtcat cgaccgccag gatagcctcc aacgcatcgc
      301 cgcactcccg cccgcgcagc gcgatgccta tatcaaacga ctcgtcagaa cacttcgccg
      361 tcaacagggc ctccgggacg agcaggatac gtccttctct gtgtcccgcc taaatagcaa
      421 caataccgtc cccgacctgt tcgccggcaa cggcgccgaa tggtatttcg ataaccagac
      481 cgccagggca aaaggatttt ccgatttcag gaccaattgg ggcaaccgcc ccaatgtcga
      541 taactgggcg atcgcgtcga tcatgagaac caatatgccc accgctccgg gagcccgaag
      601 gaccatcgac atggcggaca gcgcaggcag aatggccgcc tccacccgca tcgattataa
      661 gtccctttta ggccatctgc ccctgacacc cgacaaaatg cagaagtcga tggattccat
      721 cgaacacgcc ttgttcggcc ttggcaggag ctatcaggat ggaatagccg attatccgta
      781 cgccatcggc gcttatgact cactgctcta tcacttcccc gccaccaaac accgcgagga
      841 gtccctgttc aacctctatt actgctatag acagctgggc gatcaacccc atgccgacag
      901 tgtcctccgg ctgctgtcag aaaagtaccc gaaaagtaat tttacagccc gcatcctcaa
      961 ccccgactcc gtagaacagg cgcaaaccag ccagaaactc aacgccaccc gccagtacga
     1021 taaggtctac gtcgatttca tcgaaggccg cttcgacgac gcgctggcgg aaaaaaagat
     1081 agccgatagc ctctatggcg ataaatactg gaccccgcaa ttactctaca tcgaatcggt
     1141 ctatttcatc aaaacccgcc aggacgaaaa agcccgcgcg atcctgatga gcatcatgaa
     1201 ccggtttccc aagacgccga tggccgacaa ggccgggtcg ctcctggatg ttctcggccg
     1261 ccgccgacag atcgaagact acctgacccg tctcaaggtc acgagggcga aggatgaaga
     1321 taccgtcctc aatgccgccc ccgacaccgc agggcaggca gccgccaacc gcagaccacg
     1381 gctgatacgc aacgattcca atatgctggt caaagaagac acctcttcct gggcccgcgg
     1441 caaagcccgg gagcaggccc tcgccaaccc cgcgggtgca ccacccaaac caaacgcaat
     1501 aggacccgcc aaaatgaatg tcgacaccgg cagcatacgc aagatcagta tggatgccag
     1561 ccagttggcc agcctccatc atcttcagga ctcgctcaat agagccatgc agaaagccca
     1621 gtcggattcg gcccaaatgg cccgcctcag acaacggtcc gattcgatta ataacgcgct
     1681 gaaaaaatta cagtcggata ccgcacaatt ggcagcaaaa ttgcgaacta tgaattcggt
     1741 attcaccatg accccggaca agccgcacgc ggtgatcatc gtcctcgaca gggtggatcc
     1801 ggtatacgta tcggaagcaa aaaatgcctt cagccggttt aacctcgaaa cctattatag
     1861 caagggtctg acaacggaga atgcgacact aagcgattca ctcaaactgg tggtcatcaa
     1921 tggattcccc aatgccgggg acgccctggg ttatctgaag acggtcaaac ccgaggcccc
     1981 gagagacatc gtaccctggc tgccggctgg caaatacagc ttcctgatca tatccaacaa
     2041 caatctccag ctgctactgg ataataagga catgccggct taccgcaaat tcctgtcggc
     2101 ggcataccca acgacattct aagatccgta ccctattttc cccaaacttt gccatttggt
     2161 taacaaggat tacgatattt ttacgtcgct caaatcgcaa ctgatccata tatgtcgtcc
     2221 aatgccaacc gtattttctg gcgagccttt ttctatggcc tgggaggctt catcctcttc
     2281 ctcgtcctga tcaacctggg cgttttcggt gcacttccgt ctctgaagga gctggagaat
     2341 ccttctatca cgctcgcaac cgaggtcttc gccgaagacg gtacccccat gggaaaatat
     2401 tacaaagaca aaggcaaccg cagcaacgtc gaattcaagg atatctctcc caatgtcgtc
     2461 aacgccctca tcgctaccga agacgaacgc ttctatgaac attccggcat cgacggctat
     2521 tcagtcatgc gtgccgtact taagatgggc cgtgacgggg gcggcagcac catcacccag
     2581 cagcttgcca aaaatctgct cgaacaaggt tcgaagaata tggtccggcg tttcatcgaa
     2641 aagctcaagg aatggatcat tgcaatcaaa ttggaacgga atttcaccaa acaggagatc
     2701 ctcgcgctat atcttaatga agtgccgttc ggcgataacg tatacggcat ccgcaacgcc
     2761 gcccgaacgt tcttccagaa agagcccgac cgggtgacgg tcgatgaggc cgccatcctc
     2821 atcggtatgc tcaaaggcaa taccctctac aacccccgca gaaatcccaa aatggcgatc
     2881 gaccggcgca ataccgtcat caaccagatg gtgaagaaca actatctcac cgaagccgac
     2941 gccgccaggt tcaaggcccg ccccatcgat ctcagcaatt ataaaaagat cgacgagaat
     3001 aatggcctct caccttattt ccgcgatgtc atccgcggcg aactccaacg ctggtgcaag
     3061 gaacataaga atcctgccac cggcgagccg tacaacctct attcagacgg tctcaagatc
     3121 tatacgacca tcaacccgcg catgcagatc tatgccgacg aggccgtggc aaaacagctc
     3181 ccgatcctgc aaaaagcact cagcgctcag tatgacgtcc gcaagggcct catctggaaa
     3241 gaccacgcca atatcctcga cgccgccatg cacaatagcg accgatggca caacctcgag
     3301 gacgacggtc tcaccgatgc ggatatccgc aagaccttct accagcccgt cgccatgaaa
     3361 gt
//
LOCUS       DASYSV010000250         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_559314, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000250 DASYSV010000000
VERSION     DASYSV010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559314"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1053
                     /locus_tag="VGQ51_07695"
     CDS             46..1053
                     /locus_tag="VGQ51_07695"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV9036485.1"
                     /translation="MRPIADIYSLLNEPRRVVIIMHQKPDADAMGSSLGLSLFLRRLN
                     FQTTVISPTNWAEWLKWMPGCAEVIDYEFSTEKAVTALEEAEWIFCLDFNTLTRTRHM
                     ANRIRKLAAPKVLIDHHQQPESEVFAYGISDTEKSSTSEMVYDLIVASGHAGKLDSDI
                     ATCLYAGVMSDTGSFRFPGASAGVHRMVAALKDTGINHTRIHDELFDNFLENRLRFIG
                     FVLMNRMEIFYEYNAALIAVPWKDLVRYEIKTGDTEGLVNYPLSIQGIKMAALIIDRD
                     EEVKCSFRSKGDFDVNTFARNYFDGGGHFNAAGGRSTDTLEVTVQRFVNALNENSSLL
                     Q"
     gene            1064..2029
                     /locus_tag="VGQ51_07700"
     CDS             1064..2029
                     /locus_tag="VGQ51_07700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEV9036486.1"
                     /translation="MKTITNLLWLGLALAAVSCTNTGFKKTKSGLLYKIYSDGKGAVA
                     KKGNFLKLNFVQKVRDSVISTTEGSLPAYVPVDSSGPIYSPIEIFPMLRQGDSAVVVM
                     LVDTLIRKYGSSQLPPFLKKKDKIVFGFKVLDLYASEDLLKQDRDREMAKQKDREISE
                     VQTYLSKNNIQAQKTDKGTFYVVQSEGSGPQVDSGKEVSVRYTGKLMPSGKIFESNMT
                     GPGNAPLKFVVGTGRIIPGWDDALRKFKQGGKGTLYVPAYMAYDQQRGPGGKSFENLI
                     FEIQVDSVNDAPKVAPMPGRPDFPMPQRGAPQGRPQAAPPAQQHK"
     gene            complement(2063..>2657)
                     /gene="folP"
                     /locus_tag="VGQ51_07705"
     CDS             complement(2063..>2657)
                     /gene="folP"
                     /locus_tag="VGQ51_07705"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV9036487.1"
                     /translation="AIDLIRRRWPEIPISIDTWHARVAQEAVDAGASIVNDISGGLLD
                     ADMLPTVGRLNVPYICMHMKGDPATMRTEAVYEDVVREVLDYFIRRIEECRKAGIRDI
                     ILDPGFGFAKTSEHNWQIMRRLEVFRIPGRPLMLGVSRKSTIYRALSATPEEALNGTT
                     VAHTIGLLNGADILRVHDPKAAREAITLVRIYKEKGA"
BASE COUNT          625 a          725 c          747 g          560 t
ORIGIN      
        1 tttggcagga tggctgcggt ctggtaggcg cctgcttcat ttcagatgag accaatcgcc
       61 gatatatact ccctgcttaa tgagccccgc cgggtcgtga tcatcatgca ccaaaagccg
      121 gatgcggatg ccatgggctc ctcattgggg ctgtcgcttt tcctccgaag gctcaatttt
      181 caaacgacgg tgatctcgcc caccaactgg gcggaatggc tgaagtggat gccgggctgc
      241 gcggaggtga tcgattacga gttttccacg gaaaaggcgg tgacagcgct ggaggaggcg
      301 gaatggatat tttgcctgga tttcaacacc ctgacgcgaa cgaggcatat ggcgaaccgg
      361 atcaggaagc tggcggcgcc aaaggttttg atcgaccatc accagcaacc cgaatcggag
      421 gtcttcgcat atggcatcag cgatacggaa aaaagctcca ccagcgaaat ggtatatgac
      481 ctcatcgttg catcgggcca tgccggtaag ctcgacagcg atatcgctac ctgtctttat
      541 gccggggtga tgtcggatac aggttcgttc cgtttcccag gcgcgtcggc cggtgttcac
      601 cggatggtcg cggccctgaa ggatacgggg atcaaccaca cgcgcatcca tgatgagctg
      661 ttcgataatt tcctggagaa ccgtctccgg tttatcgggt tcgtcctgat gaaccgtatg
      721 gagatctttt acgaatacaa tgcggcgctg atcgctgtac cctggaaaga ccttgtaaga
      781 tacgagatca agacagggga tacggaaggg ctcgtgaact acccactgtc cattcagggc
      841 ataaaaatgg cagccctgat catcgatcgc gatgaagagg tgaaatgttc cttccgcagc
      901 aaaggcgatt tcgatgtcaa taccttcgcg cggaactatt tcgacggggg cggccatttc
      961 aatgccgcgg gcggacgcag caccgatacg ctggaagtga ctgtacagcg gttcgtcaac
     1021 gcgttgaacg aaaattcgtc attattacaa taactattat agcatgaaaa cgatcacgaa
     1081 tttactttgg ttgggccttg cgctggcagc agtttcctgc accaacaccg gcttcaaaaa
     1141 gaccaaaagc ggcttgctct ataaaatcta ttccgacggg aagggtgcgg tggccaagaa
     1201 aggaaacttt ctgaaattga atttcgtaca gaaggtacgg gactccgtca tctccaccac
     1261 cgaaggcagc ctgcctgcct atgttccggt ggacagcagc gggccgatct acagccccat
     1321 cgagatcttc ccgatgctgc gtcagggcga tagcgccgtc gtcgtcatgc tcgtcgatac
     1381 gctgatccgc aagtatggca gctcgcaatt gcctcctttc ctgaaaaaga aagataagat
     1441 cgtctttggt ttcaaggtgc tggatctata tgccagcgaa gacctgctga aacaggaccg
     1501 ggatcgcgag atggcgaaac aaaaggacag ggagatatcg gaggtccaga cctatctcag
     1561 caagaacaat atccaggcac agaaaacgga taaaggaacc ttttatgtcg ttcaatccga
     1621 aggttcgggc ccgcaggtgg attccggaaa agaggtgtcc gtccgttata cgggtaagct
     1681 catgccttcc ggcaagatct tcgaatcgaa catgaccgga ccgggtaatg ctccgctgaa
     1741 attcgtcgtc ggcaccggcc ggatcattcc aggctgggat gacgccctcc gcaagttcaa
     1801 acagggtggt aagggtacgc tctatgtccc ggcttatatg gcttacgatc agcaacgcgg
     1861 acccggcggc aagtcattcg agaacctgat cttcgagatc caggtcgaca gcgtgaacga
     1921 tgcgcccaag gtcgctccga tgcccggccg tccggatttc ccgatgccgc agcgtggcgc
     1981 gccgcaaggc agaccccagg ccgcacctcc ggctcagcaa cacaaataat tgaagttcat
     2041 catcaatacg cgaaaggggc cgtcaggccc ctttctcttt gtagatacgc accagcgtaa
     2101 tggcttcccg cgctgcctta gggtcatgga cacgaagaat atccgctccg ttgagaagac
     2161 cgatggtatg cgcgaccgtc gttccgttga gggcttcttc gggagtggcc gacaacgccc
     2221 gatagatggt ggatttgcgc gagacgccga gcatcagggg ccgccccggg atccggaaaa
     2281 cttccagccg ccgcatgatc tgccagttat gttcggatgt cttggcaaag ccaaaaccag
     2341 gatcgaggat gatatcgcgg atacccgcct tccggcattc ttcgatgcgc cggataaaat
     2401 aatccagcac ctccctgacc acgtcttcat aaactgcttc agtccgcatg gtcgccggat
     2461 cccctttcat atgcatacag atatacggta cgttgagccg gccgaccgtc ggcagcatat
     2521 cggcgtcgag caggccgccg ctgatatcgt tgacgataga agctcccgcg tctaccgcct
     2581 cctgcgccac acgggcatgc caggtatcga tggagatcgg gatctccggc caccgccggc
     2641 gaatgagatc gatggcg
//
LOCUS       DASYSV010000251        10903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1708578, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000251 DASYSV010000000
VERSION     DASYSV010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10903
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708578"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="VGQ51_07710"
     CDS             complement(<1..1822)
                     /locus_tag="VGQ51_07710"
                     /inference="COORDINATES: protein motif:HMM:NF020120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEV9036488.1"
                     /translation="MKAIVSFCLLQLTGLLAAAQITVGDGSVEYLTNPVGIDTREPRL
                     SWIIRGNERGIHQTAYQLFVASSPEKANEKGADIWNSGKIASDSSVGVVFAGGPLVSA
                     RRYFWRVRVWDHRGDSATMAEPAYWEMGLLTKEDWKGEWLSAPKVYDWQQFVTARKAA
                     IKAGTEEAPDPAPEFRKTFTFSKPIAQARLYISGVGYNIPYINGQKVGDHVLDPAFTR
                     YDKTVLYSTYDVTSVLHPGENVLGVALGNGWYNMITKAVWGFDHASWRNAPAVIAQLE
                     IVFRDGTRTIVSTDESWKVAPGPTGFNSIRQGESYDARKATPGWTRAGFDDGQWLRPV
                     VVAGPAGVLRAQMIQPVRITDTLIPKSITEPKPGFYVFDIGRNIAGFAELRIDAPAGT
                     EITMKYGERLFADGTVDQRDIAQHVEGGIVQTDKYICKGGGVETWHPAFSYYGFQYVE
                     VSGLPARPDRATITGMTIHTAFEPAGNFQCSNELFNKIQRNAIASYESNFMGYPTDCP
                     QREKNGWTADAHLAAEMGLYNFKAENGYSKWLGDIADEQRPTGELSAIVPSSGWGYFW
                     GNGPAWDNAMVLIPWYLYEYAGDRRIVRQLYPHIKRYVDYL"
     gene            complement(1826..3985)
                     /locus_tag="VGQ51_07715"
     CDS             complement(1826..3985)
                     /locus_tag="VGQ51_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036489.1"
                     /translation="MKKRTGYILIGLLLLAALPGVSQDSLPATTPRVKPGSFPVSVQF
                     SPVPSGKGWHDEVPPLTDQTMEETIQNIILHGCTDIAAGEFSAGVANKNVFAYAQKLG
                     MKIDWTTNGVQLFKRNDPPAHSVYSPEYIAEVKKRIGPVLGDVKKLEHPNTIFPFMDE
                     PFHADTTSFDYSPATAAGFKKQYGYTMPASYAAAKSDPRKHLDFVNFQSATFAVAWRQ
                     IYKEVKAFDPRPQIVMTHDSHSTMGGGVESDSRMAVDDIFHWGGDFADMFLYDIYPYT
                     MFDYRYGEYGRLPKPRISQTHYTMAQMRNLTTTYHKKLGFWLGTYNEGWFRRYMNSEM
                     EKEYWGERELAYTAIANGADFLITGINIPQDARHWDDFGRGMRAIQKVGAEILSAPKV
                     KSRACFLFPRTQYVQMNEEYFNVGLSFELCLRAFGEMDILHEEQIKDENLDGYDILVL
                     ADVKMLPASVAECIARFVQKGGIVLADCVPQLDEYGRPLSTMSRLFGVSSASTDRVLQ
                     KGQWNPFSMLPPQWAHGLKTPPPAPVKTFAKAAGMAYGNSYDLTVVTPRNCKVSDGHT
                     VFSMDSGEPALIQKDVGKGKSYLVGFCLQDTYFQTFAAGDSGSRAQLYQLVHDIFADT
                     KVRSHAYSSNPDMEVTVRGNDKEAFVFVINHESSHATADVTLADLGFPVGSITDVEWG
                     RPVAFTKDGDLTKFTIMAVEGTPTGVTRLLKVTPAKK"
     gene            complement(3997..5412)
                     /locus_tag="VGQ51_07720"
     CDS             complement(3997..5412)
                     /locus_tag="VGQ51_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036490.1"
                     /translation="MTKPGRVFLSMAILAACSCSCSCGKKNSGPPPPPDSSVEFSVDA
                     NTVVAANFAGFGTQYNQNVYSTFSATDGITPANIGKLEAKVKALHSQYVRVFFDSKAW
                     PTDPNYSATTADFMSSFVNTVKLAQDAGATSINITYWHTATAALMPAFADVLNDLIVN
                     HGLTAVNQVTIQNEVNSTSMTMDNYKACYASLDQALTTLGIRSKIKFVGGDLVETNQQ
                     SWFAYMAANMNALLAGYSSHIYWNDNDAAKPVSRLTGVAAIVNGLGAGVKPMYITEYG
                     VRGSVTTGVPDPGDLTGTTKPISQTLTSALENALFQVNGLNLGFTGFSRWDCYKAKYD
                     GGEQYYSCIGSGTDGYPLYPLYYMTCLFTRSCQPGWEIVKTGQGNNKAEAVAVMQDKG
                     DQNMTVYAVNSSNGSYPFVIGGLPAKKSFHLLAWNKDQQGKIGVLTDVSTDDQGVLRV
                     TIPAQSVVAATTLAVDISDIP"
     gene            complement(5434..5796)
                     /locus_tag="VGQ51_07725"
     CDS             complement(5434..5796)
                     /locus_tag="VGQ51_07725"
                     /inference="COORDINATES: protein motif:HMM:NF021028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HEV9036491.1"
                     /translation="MKKAIKNNILLMLAIGTVAALAGSCKKSKAAIDKTCTVAFILNE
                     TNNAGDTTFVTVQAGQLVQNAPVPVRTGYSFDGWYTNSADANPNPTKNTADPKFPAYD
                     ITVKPIYLDAILYARWVK"
     gene            complement(5821..7626)
                     /locus_tag="VGQ51_07730"
     CDS             complement(5821..7626)
                     /locus_tag="VGQ51_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9036492.1"
                     /translation="MKNKLLLFCCTILLLTGCKKFLVKEPLASFTDDDFWTSESSVRA
                     FAMGYYADRFPGFGNNDAGGSMSIRETLNDDFTSTSLPGFASLVTNNGGAWDNYFSNI
                     RKDNIFVDRVTAMKGFPDSTTARHWIGIARFFRALDYSNFVFDFGEVPYYSKPLTDKD
                     SDLFRPRDPVTFVMDNVLADFQYASQNVKLTDPLTGPNGLVITKDVIDAFMSRQLLYI
                     GTKIKYDPNSSSADITRAGVYLQAAKDAAERVISSGRYSVADNYQKLCSTIDIASTPS
                     VLKEMILYRQYNTGQVTHAIASGNNTNTIQGNAAPKDLIDGYLCKNGLPINTTAGVNP
                     LYLGDTTAAAQFANRDPRLANTFLTDQFHVQYLETGYSVTGYKCWKFLDVPTKDQAYA
                     VQSFNITDAPIIRLGEVMMNYIEAAAELADMGLYAVTQGDLDQTINALRKRAGDATGI
                     RLPPMQIIGGLPAVAGTVYEDANRDPSVPSFIWEVRRERRAELVYEGFRLHDLLRWRK
                     INYENTQLYPTKNLGAWITKSSVTKALVLADINGNVTSAGNVATGSGYLKVSQTVRNE
                     ANGFVLDRDYLQSVPTYQIDFYQRNGHVLTQNPGW"
     gene            complement(7647..>10903)
                     /locus_tag="VGQ51_07735"
     CDS             complement(7647..>10903)
                     /locus_tag="VGQ51_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9036493.1"
                     /translation="LLTLCLFLFITTVQALAQKTVTGKVADEEAHALPGASVQIKGTN
                     IGVTTDNDGNFTIKVNKGQTLVISFKGTVTEERIVGNASRLNFYLKRAAAALDQVVVV
                     GYGSQRKADLTGAVATIDVQKAVGSRPVPDLARDLQGSSPGLLVTTSSGDLGQAPEIH
                     LRGITGSVNAEATPLILLDNVPIPDMNMVNPEDIESISVLKDAASASIYGARASWGVI
                     LLTSKKGKKGRIHVSYNNNFAWSSPMNTPKIADGAEGAEYMLQQYRRTAPTTASFNIL
                     GAYYDDLSIARMKQWKELYGGQNLGPEMVEGRDFERRAGQTYFYRPWNIDKLFLNPAS
                     PLMKHNLSITGGDDKTTYFASFGVMDQKGLVKVAPQPDRYTRYNGTVRVETKINNWFT
                     ARGSLMEFISHKQYPNFRLANGNAPDEYWFNIYRYPETYPYGTFNGLPLKNILTELQQ
                     AHMNTRDDDQSRLSVGATATFMKGWTADFDYTYAVDNGHSNVATSPITGINSWVDPTL
                     TSVLPNYFPAEDYVIDNSTWVKRNVGKAYTTYNKEIMGHSFKLMAGGDIESYYDNFQY
                     SKAMGLLLASRPTLNLTSGAEFANGFPTHWSTLGFFGRLNYAYKDRYLLEANLRRDGS
                     SNFPTNQKWGTFPSFSAGYIISHEKFMESILDKTPISFLKIRGSWGSIGNNDIGSNSY
                     LRIMNASASNWWINGLNPTQVGVFANIASSLTWETIQTTDLGLDAKLFNNSLDIDLDV
                     YSRATKGMVTTGVEEPTTLGSAPSARNYGELVTRGWELNVTYDKTLSNGLGFSISGNI
                     SDATGKITKFANTEVSILPGGNDVPNYQGKTVGEIWGYTSDRLFNAGDFSGNNGASNP
                     TWIYGKAPNQDALNTSSSFHFGPGDVKYKDRDGNGVVDFGTGTNLHHGDMHVIGNTTP
                     RYIYGFRLGLNYKGFDLSGFLQGVAKREYWATGSLFIPGYTAGEAVYQNQMDYWTTDN
                     PNAYFPAPSNPGANNHNANWQPQTRYLLNMAYMRVKNITVGYTFPKHWLQKAGINSMR
                     IFASGENLFTADNLEMSIDPEIQQNSIEGINDAKSFGRTYPYFRTWSCGLQVDL"
BASE COUNT         2381 a         2997 c         2822 g         2703 t
ORIGIN      
        1 ccagatagtc cacatatcgc ttgatatgcg gatatagctg ccgcacgatc cgccggtcgc
       61 ccgcatattc gtacaggtac caggggatca gcaccatcgc attatcccag gcgggaccat
      121 tcccccaaaa ataaccccag ccggaagaag gtacgatcgc cgacaactct ccagtcggcc
      181 gctgctcgtc ggcaatatct cccaaccact tgctgtatcc attctccgcc ttgaaattat
      241 acaaccccat ctccgccgcc agatgcgcat ccgccgtcca cccgttcttc tcccgctgcg
      301 gacagtcggt tggataaccc ataaaattgc tctcatagga ggcaatggcg tttcgttgga
      361 tcttgttgaa aagctcattg ctgcactgga aatttcccgc tggctcgaat gccgtatgga
      421 tcgtcatccc cgtgatcgtc gcccggtccg gcctcgcggg caatccgctg acttccacat
      481 attgaaatcc gtagtaggaa aaagcgggat gccaggtctc gacgccccca cccttgcaga
      541 tatatttatc ggtctgtact attcccccct ccacgtgctg ggctatatcc cgctggtcta
      601 ctgttccgtc cgcgaatagc ctttcgccgt atttcatggt tatttcggtg cccgccggcg
      661 catcgatccg cagctcggca aacccggcaa tattcctgcc tatgtcgaaa acatagaagc
      721 caggttttgg ttcggttatc gattttggga taagcgtatc cgttatcctg accggctgga
      781 tcatctgcgc cctcagcacc cccgcaggac ctgcgaccac tacgggccgg agccattgcc
      841 cgtcatcaaa ccccgcccta gtccatcccg gcgttgcttt tctcgcatca taactttccc
      901 cctgccggat gctgttgaag ccagtaggcc ctggcgccac cttccaggat tcatccgtgg
      961 agacgatggt gcgggtgccg tcacggaaga cgatctctaa ttgcgcgatg accgccggcg
     1021 catttcgcca ggaggcatga tcgaatcccc atacggcctt cgtgatcatg ttataccacc
     1081 cattgcccag cgctactccc aggacatttt cgccgggatg caatacggat gtgacatcgt
     1141 aggtggaata aagaacagtc ttatcatacc gtgtaaaagc gggatcgagc acatgatctc
     1201 ccaccttttg gccgttgata taggggatat tgtaccctac acccgaaatg tagagtcttg
     1261 cctgcgcgat cggctttgaa aaagtaaatg tctttctgaa ttcgggggcg gggtccgggg
     1321 cttcttccgt cccggccttt atggccgcct ttcgcgctgt gacaaactgc tgccagtcgt
     1381 atactttcgg cgcgctaagc cactcgcctt tccaatcctc cttcgtcaac aaacccattt
     1441 cccagtatgc gggttcggcc atcgtagccg agtcgcctct atgatcccat acccggaccc
     1501 gccaaaaata tcttcttgcg gacaccagcg ggccgccggc gaataccacc cctaccgagc
     1561 tgtcggaagc gatcttcccg gaattccaga tgtccgctcc tttttcattg gctttttcag
     1621 gcgaagaagc aacaaagagc tggtaggctg tttggtggat acccctttca ttccccctga
     1681 tgatccagga aagccgcggt tcacgcgtat cgatgccgac gggattggtg aggtattcta
     1741 ccgagccatc gcccacggtt atctgggccg ctgccaacag ccccgttagt tgaagaagac
     1801 agaaggagac gatcgctttc ataagttatt ttttcgcagg cgtcactttc agcagtctcg
     1861 tcacgccggt gggcgtgccc tccactgcca tgatggtaaa cttggtcaga tcgccgtctt
     1921 ttgtaaatgc cacaggccgg ccccactcga catcggtgat cgagcccacg gggaatccca
     1981 ggtcggcgag ggtaacatcg gcagtagcat gggaggattc atgattgatg acgaagacga
     2041 aggcttcttt atcattgcca cgaacggtta cttccatatc gggattggag gaatacgcat
     2101 gggaacgcac cttcgtatcg gcaaagatgt catgcaccag ctgatacagc tgcgcccgcg
     2161 accctgaatc gccggcggcg aaggtctgga aataggtatc ctgcagacaa aaaccaacga
     2221 gataggattt tcctttcccc acatctttct gtatgagcgc cggctcgccg gaatccatgc
     2281 tgaagacggt atggccatcc gagaccttgc agttgcgagg ggtgacgacc gtaagatcat
     2341 aggaattgcc ataagccatg ccggccgctt ttgcaaacgt cttgacgggt gccggaggcg
     2401 gcgtcttcag tccatgtgcc cattgcggcg gcagcatcga aaagggattc cactgccctt
     2461 tctgtaagac cctatccgtg gaagcgctgc tgacgccgaa tagcctgctc atcgttgaaa
     2521 gggggcggcc atactcatcc agttggggaa cgcaatcggc gaggacgatg ccgcccttct
     2581 gcacaaaccg cgcaatacat tcggccacgg aagccggcag catcttgaca tcggccaata
     2641 ctaatatatc gtatccatcc aggttttcat ctttgatctg ctcttcgtgg aggatatcca
     2701 tttccccgaa agcccgcaga cagagctcaa agctaagtcc gacattaaag tattcttcat
     2761 tcatctgcac atactgggtc cgcggaaaca ggaagcaggc ccgggatttc acctttggcg
     2821 ccgaaaggat ctccgcccca actttctgga tcgcccgcat gcccctgccg aaatcatccc
     2881 aatgccgggc atcctgcgga atgttgatcc ccgtgatcag gaaatcggcg ccgttggcga
     2941 tagcggtata cgcaagctcg cgttcccccc aatactcctt ctccatctca gaattcatgt
     3001 accggcggaa ccatccttca ttataggtgc ccagccaaaa acccagcttc ttatgatagg
     3061 tagtcgtgag attgcgcatc tgggccatgg tatagtgtgt ctggctgatc cgcggcttcg
     3121 gcagccggcc atattcgcca taccggtagt cgaacatcgt gtacggataa atatcgtaca
     3181 ggaacatatc cgcaaaatca ccgccccaat gaaaaatgtc gtctacggcc attcttgaat
     3241 ccgactcgac gccgccgccc atggtactgt ggctgtcgtg cgtcatgacg atctgtggcc
     3301 gcggatcgaa ggccttcact tctttgtaga tctgcctcca ggccacggca aaggtggccg
     3361 actggaaatt gacgaaatcg agatgtttcc ggggatcgct ctttgcagcg gcatagctgg
     3421 caggcatggt atagccatat tgctttttaa atcctgccgc cgtagccggg ctgtaatcga
     3481 atgacgtcgt gtctgcatgg aaaggctcgt ccataaaagg gaaaatagta ttcggatgtt
     3541 ccagtttttt gacatccccc aatacgggcc cgatacgctt tttcacttcg gcgatatatt
     3601 cgggtgaata aacgctatgc gccggcggat cattgcgctt gaagagctga acgccgttgg
     3661 tcgtccagtc gatcttcatg cccaactttt gcgcgtaggc aaagacgttt ttattggcga
     3721 ctcccgccga gaactccccc gcggcgatgt cggtgcaacc atgcaggatg atattctgaa
     3781 tggtctcctc catcgtctgg tctgtcagcg gcggcacttc atcatgccag cccttgcccg
     3841 atgggacggg cgaaaattgt acggagacgg gaaaggaacc gggctttacc cgcggcgtcg
     3901 tcgccggcaa agaatcctgc gacacaccgg gcaatgctgc caataataat aatccgatca
     3961 gaatatatcc agtacgtttt ttcactgttt tcatttttat ggaatgtcgc tgatatctac
     4021 ggccaatgtc gtcgcggcca caacactttg tgccggaatg gttacccgca gcacgccttg
     4081 atcgtcggta ctgacgtccg tcaatactcc tatctttcct tgctggtctt tattccacgc
     4141 gagcagatga aagctcttct tagccggcag accgccgatg acaaacggat agcttccatt
     4201 ggagctattt acggcgtaga cggtcatatt ctggtcccct ttatcctgca tgacggctac
     4261 cgcctctgct ttattattgc cctgacccgt cttcacgatc tcccaccccg gctggcaact
     4321 gcgtgtaaag agacaggtca tgtagtacaa aggatataat ggatacccat ccgtcccgct
     4381 gccgatacac gagtaatatt gttccccacc gtcatatttc gccttatagc aatcccaccg
     4441 ggagaaaccc gtgaatccaa gatttagtcc gttgacctgg aacaaggcgt tttccaatgc
     4501 ggaggtcaaa gtctgggaaa taggcttagt cgtgccggtc agatcgccgg gatcgggaac
     4561 gcccgtcgta acgcttcctc tgaccccata ctcggtgata tacatgggtt tgacgccggc
     4621 gcccagccca ttgacgatgg ccgccacgcc ggtgagccgg ctcacaggtt ttgccgcatc
     4681 gttatcgttc caatatatat gtgacgaata acccgccaac agagcgttca tattggcagc
     4741 catataagcg aaccagcttt gctgattggt ctcaacgaga tcgcccccga caaattttat
     4801 tttgctccgg atgccgaggg tcgtaagcgc ctggtcgaga ctggcataac acgccttgta
     4861 attatccatg gtcatgctcg tgctgttgac ctcgttttgg atggtgacct ggttgaccgc
     4921 cgtcagtccg tggttgacga tcaggtcgtt caaaacatcc gcaaaggctg gcatcagcgc
     4981 ggcagtggcg gtatgccagt aggtgatatt tatgctggtg gcgcccgcat cctgcgccag
     5041 cttgacggtg ttcacgaagg agctcataaa atccgccgtc gtcgccgaat agttgggatc
     5101 ggtgggccag gccttggagt cgaagaagac acggacatac tgcgaatgca gcgcttttac
     5161 ttttgcttcg agctttccaa tattggcggg tgtgatccca tccgttgcac taaacgtgga
     5221 atacacattc tggttgtatt gtgttccgaa ccccgcaaaa tttgcggcaa cgacggtatt
     5281 ggcgtccacc gaaaattcga ccgaactatc gggcggcggc ggcgggccac tatttttctt
     5341 gccgcaggag caggaacagg aacaagccgc caatatcgcc atcgacagga ataccctacc
     5401 gggctttgtc atttttttca tctttaaaaa gcgctatttg acccatctgg catagaggat
     5461 cgcgtccagg taaatcggtt tcactgtgat gtcgtaagcc gggaacttcg ggtcggccgt
     5521 attcttcgtc ggattgggat tcgcatcggc cgaattggtg taccagccgt caaagctata
     5581 ccccgttctt acaggtacgg gagcattctg caccagctga ccagcctgca ccgttacaaa
     5641 agtcgtatcc cccgcattat tggtctcatt gaggataaag gcaaccgtac aggtcttgtc
     5701 gatggccgcc ttgcttttct tacaggaacc tgccagcgca gccacggtcc cgattgccag
     5761 catgagcagt atattatttt taatcgcctt cttcatgatc gtatttttga aagtttgtaa
     5821 ttaccagccc gggttttgtg tcagaacatg gccatttctt tggtaaaagt cgatctggta
     5881 ggtaggtacg ctttggagat agtccctgtc gaggacaaag ccattggcct cattgcgtac
     5941 cgtctgggaa accttcagat agccgctgcc cgtcgccaca ttgccggctg aggtgacatt
     6001 gccgttaata tcggccagga ccagggcctt cgtgacggag ctttttgtta tccaggcgcc
     6061 caggttcttt gtcggataaa gctgtgtatt ttcataattg atcttcctcc agcgcagcag
     6121 gtcatgcagg cggaagcctt catacaccaa ctccgctctt ctttccctgc ggacttccca
     6181 gataaacgaa ggcacggagg gatccctgtt ggcatcttcg tataccgtcc ctgcgacagc
     6241 ggggaggccg ccgatgatct gcataggtgg aagtcttatc cccgtcgcat cgcctgctct
     6301 tttcctaagg gcgttgatcg tctgatcgag gtcgccctgg gttaccgcat aaagacccat
     6361 atcggccagc tcggcggccg cttcgatata gttcatcatg acctcgccca accggatgat
     6421 cggggcgtcg gtaatattga agctttgtac cgcatatgcc tggtcctttg tcggcacgtc
     6481 caggaatttc cagcatttat agcctgtgac ggaataaccc gtctccagat attgcacgtg
     6541 gaactggtcg gtgagaaagg tattcgcgag tcgcgggtcc ctgttggcaa attgagctgc
     6601 agcggtcgta tcgcccaggt atagcggatt cacgcctgcc gtcgtattga tcggcaagcc
     6661 atttttgcaa aggtacccgt ctatgaggtc cttgggcgcc gcattacctt gtatcgtatt
     6721 tgtattgttg cccgaggcta tcgcatgcgt tacctggccc gtattatatt ggcggtacag
     6781 gatcatttcc ttgagtacgg aaggcgtcga ggcgatatcg atcgtgctgc aaagtttttg
     6841 atagttgtcg gccacggaat accggccgga gctgatcacc ctttctgccg catcctttgc
     6901 cgcctgaaga tatacgccgg cccgggtgat atcggccgaa gaggagttgg ggtcatattt
     6961 tatttttgtc ccgatataca gcagctgcct tgacataaag gcgtcgatga catcctttgt
     7021 tatcaccagt ccgtttggcc cggtgagcgg gtcggtcagc ttgacattct gagaagcata
     7081 ttgaaaatcg gccagcacat tatccataac gaacgtcacg ggatcccgcg gccgaaacag
     7141 gtcggaatcc ttatcggtca atggtttgct gtagtaaggg acttccccga agtcgaatac
     7201 gaagttggaa tagtccaatg cccggaagaa acgggcgata ccgatccaat ggcgcgccgt
     7261 ggtggaatcg ggaaacccct tcatcgccgt gacccggtcg acaaaaatat tgtccttccg
     7321 gatattcgaa aaataattat cccaggcgcc gccgttgttg gtcaccaggc tcgcaaagcc
     7381 gggcaggctg gtgctggtga aatcatcgtt gagcgtctcg cggatggaca tggacccgcc
     7441 cgcatcattg ttcccgaaac ccgggaaccg gtcggcataa tagcccatgg caaatgcccg
     7501 gacactggat tccgatgtcc agaagtcatc gtctgtaaac gatgccaatg gctcctttac
     7561 gagaaacttt ttgcacccgg tgaggagtaa gatcgtgcaa caaaataaca atagcttatt
     7621 tttcataacc gtctgaaatt atgtgtttat aaatctacct gtaacccgca tgaccaggtc
     7681 ctgaaataag gataggtcct tccaaagctt tttgcgtcat tgatcccttc gatcgaattc
     7741 tgttggatct ccggatcgat ggacatttcc aggttatcgg cggtaaaaag gttctcgcca
     7801 ctggcaaaga tccgcatgct gttgatcccg gctttctgca accagtgctt cgggaaggta
     7861 tagcccaccg ttatattctt gacccgcata tacgccatgt tgagcaaata gcgggtttgc
     7921 ggctgccagt tggcattgtg gttattcgct cccgggtttg acggcgctgg gaaataggcg
     7981 ttcgggttat ccgtggtcca atagtccatc tggttttgat aaaccgcctc ccccgccgtg
     8041 tagccgggaa taaagagcga tcccgtcgcc caatattccc ttttggcgac gccttgcaag
     8101 aatccgctaa ggtcgaaacc tttataattc agtcccaacc ggaagccgta aatgtagcgg
     8161 ggagtggtat tgccgatcac atgcatgtcg ccatggtgaa ggttggttcc ggttccgaaa
     8221 tcgactaccc cattgccatc cctatcctta tatttgacgt ccccgggacc gaaatgaaaa
     8281 gaggaactgg tattcaacgc atcctggttt ggcgctttgc catagatcca ggtaggattc
     8341 gatgcgccgt tgttacctga aaaatcgccg gcattgaaaa gcctgtccga agtgtaaccc
     8401 catatctcgc cgaccgtctt accctggtaa ttcgggacat cgttaccgcc tggcaggatg
     8461 cttacttcgg tgttggcaaa ttttgtgatc ttaccggttg catccgagat attaccgctg
     8521 atgctaaacc cgagcccatt ggacagcgtt ttatcatacg ttacgtttag ctcccatccc
     8581 ctggtgacca gctcgccata gttgcgggcg gaaggggcgg agcccagggt agtgggttct
     8641 tcgacccccg tggtgaccat gccctttgta gcgcggctat atacatccag gtcgatatcc
     8701 agcgaattat taaacaattt ggcgtctagt cccagatccg tggtctggat ggtctcccag
     8761 gtcagcgacg aggcgatgtt cgcgaatact cccacctgcg tgggattgag tccattgatc
     8821 caccaattcg acgcagaggc attcatgatc cgcagataag aattgctgcc gatatcgtta
     8881 ttaccgatgg atccccacga accgcgtatc ttcaggaaag aaatgggtgt cttgtctaaa
     8941 atagactcca tgaacttttc atgcgagatg atatatccag cggaaaaaga cgggaacgtg
     9001 ccccatttct ggttggtggg gaaattggaa gacccgtccc ttctcaggtt ggcctccagg
     9061 agataccggt ctttataggc gtagttcagc cgcccgaaga atcccagggt cgaccaatgt
     9121 gtggggaacc cgttggcaaa ttccgcaccc gaggtgaggt tcaaggtcgg ccgcgacgca
     9181 agcagcagtc ccattgcttt ggaatactga aaattatcgt agtaagactc gatgtcaccg
     9241 ccggccatca acttgaagct atgccccatg atctccttgt tgtaggtcgt ataggccttg
     9301 cccacattcc ttttgaccca ggtggagttg tctatcacat agtcttccgc gggaaaataa
     9361 ttcggcagta cactggtcag cgtggggtcc acccagctat taataccggt gatgggtgag
     9421 gtagcgacat tggaatgccc gttatctacc gcatacgtat aatcgaaatc tgccgtccaa
     9481 cccttcatga aagtggctgt ggcgccgaca gacaggcggc tttggtcgtc gtcccttgta
     9541 ttcatatgag cctgttgcaa ttccgtaagg atgttcttca ggggtaagcc gttgaaagtg
     9601 ccatacggat aggtttcggg ataacgatag atattgaacc aatattcgtc cggggcattc
     9661 ccattggcga gcctgaagtt gggatattgt ttatgcgaga tgaattccat cagtgacccg
     9721 cgcgcggtga accaattatt gatctttgtc tcgaccctaa ctgtcccgtt gtaccgggta
     9781 tacctatccg gttgcggagc cactttaacc aggcccttct ggtccatcac accgaagctg
     9841 gcgaagtagg tggtcttatc atccccgccg gtaatcgaca aattgtgttt catcagcggt
     9901 gaagccgggt tgaggaagag cttatcgata ttccagggtc ggtaaaagta ggtctgaccg
     9961 gcgcgccgct cgaagtcccg gccttcgacc atttcggggc cgaggttttg gccgccataa
    10021 agttccttcc actgcttcat ccgcgcgatg ctcagatcgt cataataagc tcccaggata
    10081 ttgaaactgg ctgtcgtcgg agccgtcctt ctatattgct gaagcatata ttccgcccct
    10141 tccgcgccgt ccgcgatctt tggcgtgttc atcggggatg accatgcaaa attgttgtta
    10201 taagaaacat ggatccggcc ctttttacct tttttggacg tcaggaggat gactccccag
    10261 gacgccctgg ctccatagat ggaagcggaa gccgcgtcct tcagcacgga aatggattca
    10321 atatcctcgg ggttgaccat attcatgtcg gggataggga cgttatcgag caggatcagc
    10381 ggtgttgcct cggcattgac ggaaccggtt atccccctga gatggatctc gggtgcctgg
    10441 ccaagatcgc cgctggaagt ggtgaccaat aacccgggtg acgatccctg gagatcgcgt
    10501 gccagatcgg gtaccggtcg cgatcctact gctttctgta catcgatggt ggcaacggcg
    10561 ccggtcaggt cggcctttct ctgactgcca tatcccacta ccacgacctg gtccaatgca
    10621 gcggcagccc tttttaaata aaaattaagc cgggaggcat tacctacgat gcgttcctcc
    10681 gtgaccgtac ctttaaagct gatgaccagg gtttgcccct tgttaacctt tatggtgaaa
    10741 ttcccgtcat tgtcggtggt gacgccgata ttcgtgcctt tgatctgaac cgaggcccct
    10801 ggcagcgcat gcgcttcctc atccgcgacc ttgccagtaa ccgtcttttg tgccagggct
    10861 tgtactgttg taatgaacag gaacaggcac agcgtgagca atc
//
LOCUS       DASYSV010000252         5073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1709070, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000252 DASYSV010000000
VERSION     DASYSV010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709070"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..668
                     /locus_tag="VGQ51_07740"
     CDS             135..668
                     /locus_tag="VGQ51_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEV9036494.1"
                     /translation="MAEMDTSSGGGHKKGPGVKKGKKLSTRVDLTPMVDLGFLLITFF
                     IFTTTMSQPTAMRLFLPKDVDKPEEQNKVKESAVLTLMPSKADQIYYYEGADPSKLQS
                     SGYKGIRDIILDKKRRTDPKDFMVVIKPTKDATYKNTVDMLDEMTIDAVKRYALVDIT
                     PVEYGFVEKTETANGVK"
     gene            752..1582
                     /locus_tag="VGQ51_07745"
     CDS             752..1582
                     /locus_tag="VGQ51_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEV9036495.1"
                     /translation="MEVNKILNADILDIIFEGKNKEYGAYELRKTYNTRLVRALIGTG
                     VIIVLMFVGHLLSNMNNGPKKQAVVVEDVQLEDIKEQKKDQPPPPPPPKVEPPKVEMA
                     KFTPPKIVKDNEVKPEEKPPEQEKLEDTKIGAVNQEGIKDEGITAPPVSDAGKGVVEA
                     PKKDETDYDKTFTKVEIESDFPGGSAAWLRYLNKNLRYPDDAVNNEIQGTVVVQFIVD
                     KEGNVSEVQAISGPENGGLREEAVRVIKKSGKWTPAIQNGRQVKSYKKQPIVFKLEAQ
                     "
     gene            1792..2607
                     /locus_tag="VGQ51_07750"
     CDS             1792..2607
                     /locus_tag="VGQ51_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEV9036496.1"
                     /translation="MESNKILGADILDILFEGRNKEYGAYELRKTYNRRLGRAMAVTG
                     SVILLLFVSGFVGKHGAKKVETPKIADIDLTAVKAPDPTPPVIPPAPKAPQVKTVIFT
                     PPKIVPNDKVPDDEKPPVQDDLDKVKIGTVNTPGADDPGITAPPSDQGKTGVVVAPKK
                     QDDDDGIFEKVEVESSYPGGVPAWIRYLQKNMHYPDQAVNNETQGTVMVKFIVDKDGN
                     VSDVEAVAGPEQDGLREEAVRVIKKSGKWTPALQNGRYVKSYKQQPVTFKLSE"
     gene            2806..4197
                     /gene="mnmE"
                     /locus_tag="VGQ51_07755"
     CDS             2806..4197
                     /gene="mnmE"
                     /locus_tag="VGQ51_07755"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV9036497.1"
                     /translation="MLGKLAGWGDTIVALATPPGIGAIGVIRVSGSRSLDVVGRLFVG
                     KDLAAQPSHTLHLGLLKEGDEQLDEAVVSLFRAPRSYTGEDVVEISCHGSPVVLERVM
                     QALLRQGTRMARAGEFTQRAFLNGKMDLTQAEAVGDLIASQSASSARAALHTIRGGFS
                     RALKELRERLVSFSALIELELDFSQEDVEFADRTQLYHLISEAEEETSALLASFRLGN
                     VIRNGVSVAIVGKPNAGKSTLLNTLLNENRAIVSEIAGTTRDTIEEVLNIDGILFRLI
                     DTAGIRHHTSDVIEQMGVGRSLEKMEQADIVVYLFDVGSDDAATVGAVREELVAKGKP
                     FLLVGNKVDGLDGSSALAASGRWPQELLISAREGRHIDILKKQLVDQVMEGAGLGAEG
                     GTIVTNARHYQALKQVGESLETIHKGLDELLPGDLLALDIRHCLHYLGEITGEVTSEE
                     RLGYIFSKFCIGK"
BASE COUNT         1291 a         1005 c         1604 g         1173 t
ORIGIN      
        1 gttaaggttc aatagattat tgaagtatta cacatgagaa agcggccgat ggccgggttg
       61 ggccgggatt ttgccggcgt gggctttcgg cgaaaaaaca gtaactttaa gtaacttaaa
      121 aaaagctttt tactatggca gaaatggata cctcttcagg cggtggtcat aaaaagggcc
      181 ccggtgtgaa gaaagggaaa aagctctcca cacgggtgga tcttacgcct atggtggatc
      241 tgggattcct gctgatcacg ttctttatct ttacgacgac gatgagtcag ccgacggcca
      301 tgcgtctgtt cctgccgaag gatgtcgaca agccggagga gcagaacaag gtaaaggaat
      361 ccgcggtttt gacgctcatg ccttcgaagg cggaccagat ctattattac gagggtgccg
      421 atccttccaa gctgcagagc tccggttaca aggggatccg ggacatcatc ctggacaaga
      481 agcggaggac ggatccgaag gatttcatgg tggtcatcaa gccgaccaag gatgctacgt
      541 ataagaatac ggtagacatg ctggacgaga tgacgatcga tgcggtcaag cggtatgccc
      601 tggtggatat tacgccagtg gagtacgggt tcgtcgaaaa gaccgagacg gccaatgggg
      661 taaaataaat ccctcggggg gttatggaat ccttaacaga tggcatcata aaggcgggcc
      721 gggggcccaa aatatattaa aataatttat aatggaagtc aacaaaatac taaacgccga
      781 tatccttgat atcatcttcg aggggaagaa caaggagtat ggcgcgtatg agttgcgcaa
      841 gacctataac acgcgcctgg tgcgggcgtt gataggtacc ggggtcatca tcgtcctgat
      901 gttcgttggg catctcctgt ccaatatgaa caatggcccg aagaagcagg cggtggtggt
      961 cgaggatgtg cagcttgaag atatcaaaga gcaaaagaaa gatcagccgc cgccacctcc
     1021 gcctcccaag gtggagccgc cgaaggtgga aatggccaaa ttcacgcctc ccaagatcgt
     1081 gaaggataat gaggtgaagc cggaagagaa gccgccggag caggagaagc tggaggacac
     1141 gaagatcggc gccgtcaacc aggaaggtat caaggatgaa ggtatcactg cgccccccgt
     1201 cagcgatgcc ggcaagggtg ttgtggaggc tccgaaaaaa gatgagacgg actatgacaa
     1261 gacctttacg aaggtggaga tcgagtcgga ttttccggga ggtagtgctg cgtggttgcg
     1321 gtatctgaac aagaacctgc gttatccgga tgatgcggtg aataatgaaa ttcagggtac
     1381 tgttgttgtg cagtttattg tggacaagga ggggaatgta agtgaggttc aggcgataag
     1441 cggccctgaa aatggcgggt tgcgtgagga ggctgtgagg gtaattaaga agagtggtaa
     1501 gtggacgccg gctattcaga atgggcggca ggtgaagagt tataagaagc agccgatcgt
     1561 gtttaagctg gaggcgcaat agattgtgat gagataaatt tttgtgaggg ctatgccgaa
     1621 agattttgtg aggcccccgc cgaaggcggg ggccttttta tttgccggcg tgttctcggt
     1681 tgtacgtaaa cgttatggaa tcgggcatgg atcgcatctt atgacaagcg agcctcaagc
     1741 gaagcttgcg attgagccct tgccggtggc aagggagaaa atcaaattat tatggaatcc
     1801 aacaagatcc tcggcgccga catcctcgac attttgttcg aggggaggaa taaagagtac
     1861 ggcgcgtatg agttgcgcaa gacgtataac aggaggctgg gtcgcgcgat ggctgtgacg
     1921 ggctctgtta ttcttctgtt gtttgtgagc gggtttgtgg ggaagcatgg agcgaaaaag
     1981 gtcgagactc cgaagattgc agacatagac ctgactgctg taaaggctcc tgatccaacg
     2041 ccgccggtga tcccgccggc cccgaaagcg ccgcaggtga agacggtgat ctttacaccg
     2101 ccgaagatcg tgccgaatga caaagtgccc gatgacgaga agccgccggt gcaggatgat
     2161 ctggataagg tgaagatcgg gacggtgaat actccgggtg cagatgatcc agggattacg
     2221 gcgccgccat cggatcaggg aaagaccggg gttgttgttg ctccgaagaa gcaggacgat
     2281 gatgatggga tatttgagaa ggtggaggtg gagtcttcgt atccgggagg ggttccggcc
     2341 tggatccgtt atctccagaa gaatatgcac tatcctgatc aggcggtgaa caatgagacc
     2401 cagggtacgg tcatggtgaa gtttatcgtg gataaggatg gaaatgtcag tgatgtggag
     2461 gcggtggccg ggccggagca ggatggattg cgggaggagg cggtacgggt tatcaaaaag
     2521 agcgggaaat ggacgccggc tttgcagaat gggaggtatg tgaagagcta taagcagcag
     2581 ccggtgactt ttaaattgtc agaataacgg gtgtggtgag ggtcgcggcc cggggatgta
     2641 tggttttgaa acgattttga cttacttact agtaagtaag taaatgagga cccttctcgg
     2701 ggtccttttt aattgggtgg cgtacttgag gtagcgggac ccggaagttg agatcttgca
     2761 aaccgcggtt tgcttcacga tggccggcgg agttatcttt gcagtatgtt aggcaaatta
     2821 gcaggctggg gagacacgat cgtagcgctt gccacgccgc ccggtatcgg cgctatcggg
     2881 gtcatccggg tgagcgggtc ccggtctctt gatgtcgtgg gacgattgtt cgtcggcaag
     2941 gatcttgccg cccaaccttc tcataccctg cacctgggac tattgaagga aggcgatgag
     3001 cagctggatg aggcggtcgt ttcactattc cgcgccccac gcagttatac cggcgaagac
     3061 gtcgtcgaga tctcatgtca tggcagcccg gttgtcctgg aacgcgtgat gcaggcgttg
     3121 ctgcggcagg gtacccggat ggcccgcgcg ggcgaattca cacaacgcgc ttttctaaac
     3181 ggcaaaatgg acctgacgca agccgaagcc gtcggcgacc tcatcgccag ccaatccgca
     3241 tcatcggcac gcgccgcgct tcatacgata cggggagggt tttcgcgtgc tttgaaagaa
     3301 ttacgcgaaa gactggtcag cttctcggcg ctgatcgaac tggagctgga tttttcacag
     3361 gaggatgtgg agtttgcaga caggacacaa ttgtatcatt tgatctcgga ggcggaggag
     3421 gagacatcgg cgctgttggc ttcgttccgc ctggggaatg tgatccgcaa tggtgtttcg
     3481 gtagcgatag tggggaaacc caatgcaggg aaatctactt tactgaatac attgttgaat
     3541 gagaatcgcg cgatcgtgtc ggagattgcg gggacgacga gggatacgat cgaagaggtg
     3601 ttgaatatcg atgggatact ttttcggctg atcgatacgg cgggaatacg gcatcataca
     3661 tcggatgtca tcgagcagat gggggtgggg cgtagccttg aaaaaatgga acaggcggat
     3721 attgtggtgt acctgttcga tgtcggctcc gacgatgcgg caacggttgg tgcggtgcgt
     3781 gaggaactgg tggcgaaggg aaagccgttt ttattggtgg ggaataaggt ggatggattg
     3841 gatgggtcct ctgcgttggc agcttcgggt cgatggccgc aggaattgct gatatcggcg
     3901 cgggagggaa gacatataga tatactgaag aaacagttgg tcgaccaggt gatggaaggg
     3961 gctggtttgg gcgcggaagg ggggacgatc gtgacgaatg caagacatta tcaggcgttg
     4021 aagcaggtgg gggagtcgct ggagactatt cataaggggc tggatgaatt gttgccgggg
     4081 gatctgttgg cgttggatat aagacattgt ttgcattatt tgggggagat tacgggggag
     4141 gtgacgagtg aggagaggtt ggggtatatt tttagtaagt tttgtattgg gaagtgaccg
     4201 cacttacggg attgtacggt ttgtacgggt tatattcttt tggcgctgat tctcagcgct
     4261 tttcttttgc ctttatttgc cgattttggc caatagtggc cattttcttg ttactaaagt
     4321 gggacgaacg atggttttgg ggtgatcttc aggttaggaa ggggagatta tagcagcgaa
     4381 acgctcgcaa agtcagcttt tcgcatacaa atgccctctt tctcaagaga ttaaccacca
     4441 aaatccaaag aaatgagtag taacattcga attacccgga tttgtgagca ctgtgggctc
     4501 gaattcacgg ccaagaccgt ccgtacccga tattgttcgc atgcctgtaa cagaaagggc
     4561 tataagaagg ttgagcgaga taggaagctg ggcgcagcca aggctcgtat aagggttgag
     4621 cctttgaatc tacggcatga tctcgtcaat tgggatgaat tgacccaaaa gccttatctg
     4681 acggtttctg aagcctgcct ggtattgaat gtgagggtgg agaccttacg gagatgtata
     4741 aaggacggaa tagtcaagac taaacggata gggaaaaagc atctgattaa ccggcaacat
     4801 ttaataagtt attgaacaca ttgatagcca aggcatagtg gctttcttat aataaaatgc
     4861 taatatcatt agttaaaata gtgtgtctct cgtagaagtt ttgacactta aaaaaagcct
     4921 attatgagct atacagaagc agacatggtt taccatgact acaaaagaac tgctagagct
     4981 gaccacgaca acccttatta taagcatggt caggaggggg ccgagctcaa tcgaaccgag
     5041 ttgtacgaaa tgctctatta tgtcaatcac ctt
//
LOCUS       DASYSV010000253        15076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2351496, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000253 DASYSV010000000
VERSION     DASYSV010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 15076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15076
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2351496"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VGQ51_07760"
     CDS             complement(<1..772)
                     /locus_tag="VGQ51_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036498.1"
                     /translation="MSFTMNWLFDWLWKRRRVPRAALAASGDVALTAMVSEEEEGKSQ
                     VGSRSEDDDDASADDPDDASADDARADDAPTDDADEASADDTDDASTDDGDNDNDDER
                     AAGEDAPEVRSVPVITSPGPLPIELKYLDELIRYRIGQVLHPHLHHAPPPMPKIEHWQ
                     LPIGQFILDYNKSNTPKLTDDDARLLLIALVDHVQPDLFDYSIHVALKGEGDFQPIGG
                     TRGKNFRGFIPTGQTAVFLLGGVNWERDIRFQKLFWADH"
     gene            complement(782..1483)
                     /locus_tag="VGQ51_07765"
     CDS             complement(782..1483)
                     /locus_tag="VGQ51_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEV9036499.1"
                     /translation="MSYSLRNYRHRNPKAGDEQKTNSGPFFQTGKGAAVQRKKKSGFF
                     VQRLATSKEDEKLGTNDARMEKDKEEPMKPVQTKQAPGDKKKKKKPVQKKGHNMDKRK
                     KKHIRRAEDPEKKKKKAPGGGGMIHKKEKTHEAEGHSSMHAVEAELEHQSGKGSALPA
                     KTLAEMNHSFGTDFSHVRVHHDATAAALCNELNAQAFTHGCDIYFNEGKYNPESEEGK
                     RLLAHELTHVIQQTS"
     gene            complement(1502..2281)
                     /locus_tag="VGQ51_07770"
     CDS             complement(1502..2281)
                     /locus_tag="VGQ51_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEV9036500.1"
                     /translation="MSVISIAIQKGGSGKTTTAVNLGAALTRAGKKVLVVDADPQANL
                     SQALGIEDEPVYNLYTELKKEIQGDGGDLRQTIVHSVAGPDVVPASIDLAIAELELVS
                     VYGREQLLKGLLQPLLQEYDIVLIDCPHAIGMLTVNALVASDLVLIPLPGEFLPLKGV
                     YSFMRQFDMIRRKLNVQLQLLGIVLTKYDDRKLMNLSVKQQLEGKFPGKVFEGVIRTN
                     IQLVKAQEVGKDIFSYDKSSNGAKDYQGLADELLKKLEKNN"
     gene            complement(2292..2723)
                     /locus_tag="VGQ51_07775"
     CDS             complement(2292..2723)
                     /locus_tag="VGQ51_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036501.1"
                     /translation="MLTLLITLNTPTVSAGEMPELTARLINEGDDILLVNSRMLLVPA
                     AMPDDRKEMVIHIDGPPDSMNLMRVHVQAKPPLPGDFVRLFPGEYLYSVYTLEEHFSY
                     EEPGTYRVFAEYFNQIDLVVDGIRSWKGHIRSNNASFGINV"
     gene            complement(2717..3676)
                     /locus_tag="VGQ51_07780"
     CDS             complement(2717..3676)
                     /locus_tag="VGQ51_07780"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV9036502.1"
                     /translation="MNNYERYSSRQLYDAANTGPENLRSYLIGLLMQREDRDPYIREV
                     LASSDRELLRAGLRSLSPSDAVRFGPALEGLLSTADQDIRRTIVKKWGEAREKTAIPF
                     LLALIDNVNEDPLLKKMAIFSLGEIGGEAAISPLSALFGEKDADTKIAILIALNKIDH
                     EGSHPLFIRASTDRSPDVRHWAEEFLKSPPPAPVKDDHSITRTWMRDPLAWPDRDRLS
                     FLSNGEGVYEEDKAGETTLLLNFRYRLETSRQIVILSRGDSEYATGYTISNDIFHHPY
                     EGKIPCLTLEFADKKIAIDGIVLTGTPYYFLAKESTNNARTLC"
     gene            complement(3684..7664)
                     /locus_tag="VGQ51_07785"
     CDS             complement(3684..7664)
                     /locus_tag="VGQ51_07785"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEV9036503.1"
                     /translation="MKTMESHRSTSAANKSGPFFQKSRGNGFFQPSRSGGFFSGGAAG
                     SGGMAPSGIVQTKLTIGQPHDVYEKEADSVADKVVQRLSRGGESHEPAPAPAPAVTPF
                     MPAGANAAPIQECAACDKEDKLDRKEEETGGEEKGKIRKKPIFQSNEDPPEDDHPIQR
                     KCKHCEEEEKKVQRKCKHCEEEEKHLQKKGADGAAASPSIESSLSSHRGGGSPMSHDT
                     NSSMSQAFGADFSGVRIHTDSSAAAMNNELNAHAFTHGSDIYFNSGKYDDRSKEGKKL
                     LAHELTHVVQQNGDTLRRNLWDDFKSDVSAGVDAAGQGLSDAGHAIAGAAEDTGEALY
                     HTGQAVVEGAKDTGQALYNAGSGAVQQIASAGSQAMDWLQSEAGQAASALADFFGVDI
                     HITATGGLEIILPRTCPINAITDRSVLDPWEKGFLVPIVNFEILPGLFITGSIGLKGS
                     LTPEIQTQLGPFCLEGARILIDPLSNHYSVSGAISMAAAASLGAEIRGGVQGELRLNG
                     VVFVGAIPIPVDFPLIEAEGGLAGLVRGIGVGRLTLGGSLDYSGGTITAGGFGRLELG
                     LGADLFAGAYAQLKLLDKYLCRIYWQPYEWHGGIAYSFDLSGGVHVGTGALPGVGGCH
                     VIKPKMDEIPFDQLPLAISREGFSDDCPLLDMLCEYLRDKKLLPSQNGGVWDPTGTGH
                     GGKYGPGPRLAGPLNVYQRDPKIVSGASCRGACGVNCDTCEVYPSYTFTDPVTGEVWL
                     YTNFQDCDTNGGCREHDAAFDWAAAEKGETGKGAMFMPWHMAATIECTCNNLAGNCIA
                     WAFGLPPFDGKLYFADKAEPLSGVLAQNECRDANPDFTDCTEDGSDRDRVLSEWAAKN
                     GYKDVRDCKKFDKFPPYKSGAPREVCGEDVPGETWHCTATEIATGKSVTISIFGCFCC
                     NDDTGWSGIDWRSPHVSIRLTGTAAFSRDVPMIETKTFNDVQRLMRSKRYADALDVLM
                     KDLERNKHVDPAKFTIRFVNRTDKGEGLTNTKFHKNPATGKMDPSGPSRVTIYTPAFG
                     SIQELVSTVMHEYQHVLQDQVPGQDPAKFIDEAHGGHAGEELEVAETEAYLWEIENSD
                     QTGLASRDRDMLDIARRLTDHYDGIGGFSRARQKIYEVRYNKAQQFVKDLVAPPPPMP
                     GYSHVYHHGTNYDDVEKLRTVDIKGVSPVDFGGAFYTHTKGNWRLARDWSIRGSLGQR
                     GWGVITFPIPDSFWTAYIHSELYFRNTRSKPANMPINPDTGKPFKDWKDFVEYNKREN
                     RKGNLPDWGARAGFDVIEGPLWGSLAKKPNIHQVAFTSKGVDVLNDPAVKKLRFLRKW
                     LFLRFR"
     gene            complement(7693..10932)
                     /locus_tag="VGQ51_07790"
     CDS             complement(7693..10932)
                     /locus_tag="VGQ51_07790"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEV9036504.1"
                     /translation="MKTAVSKIPASSAKTVSPFFSKERGQGFFSPSGHQRGRKGNAGG
                     AFFSQAGPVQTKLTVGQPGDVYEREADQVADKVVQRMSRPIFESKAERKEEGIQRKCA
                     ACEKEEKLQKKENAGANQTASPHTEQQLHASHGGGSALSSADRAPMEQTMGADFSGVR
                     IHTGSQAQQMNEQLNAQAFTHKNDIYFGKGKFDTGSKAGQRLLAHELTHVVQQGGAAV
                     KTAPAVPDQDIGGQNLPGTNVQPESTQAAPNAAGGAASAIPQTATAKTAAGTPSAAQG
                     KATAGGPATQTATSGPATQAAPNGPVTGQEPKTGDLPSEQMGRLTHAHNSVYKIAAVR
                     QAAHLEQDVVLSRALIDQIAVARTKEIRQWFDGRRMEINQFFIGTGQDVLNTIQQKQI
                     QFMQMSFNAMNRVQAFVTDAEQGTIKLGDLFRDQLSAFIDGISNGVRDSINDIAGTIT
                     GLISSVHLPDLPGVGMVRDMIVSILNQAASAARGVISLVTSALQGALTKASQMITDVI
                     RTAGQLIRQLVNKIFEVILGIQQTLFMAVQRVGNMILSQMNSFLQGTLWPILQEGEDF
                     LVEQVEEARLIRLMQLDDNEAQVLKAMADVIDPKTKPQKKGAATTSREQRLLYFTQVE
                     QVGVRRNAEIVANFVAETSSFFMIAIHQILAFSEFVYHAWQTALRQFDENISSMVNSA
                     IQKVGDFISGEIDKIKTLFSKAVDNALELVSKFILLVGKVKDAIVKILTSSVSAIGSY
                     IGRLVVQFVSGGGTGADVAAAFTGGGGSALANLPAPQTTPEWEELITELEELEPLFDQ
                     TLKLAGEELAVVEEATLVAEEAETAAEVVVDGWEVVLIILIIVLILVIIALVVYLLYL
                     LIEELLKPVPTPEPIPIPEPEPAPEPDPDDPDENDCNDDEFPDPTAPTGMTPADPIDI
                     IWFKPLNQYENPIVLKGTTFTMTSRKPLPPPHQTDTIGVLGPFLPAHHKALQIDKNNK
                     RRTQQRRFKRVLASLGFSWKNSAGDNLSPDHVQDLFWEGPDDFANLWPLESAYNERAG
                     SIQNNAQPIYFRPDPARPCAIEIPIGTAIKRFPNMVIRHYTIDDIRPLP"
     gene            complement(10971..12107)
                     /locus_tag="VGQ51_07795"
     CDS             complement(10971..12107)
                     /locus_tag="VGQ51_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9036505.1"
                     /translation="MHKPKEPALQAKEKPPFFGGQGFFSAAPAPMIQRDIGQSIPVAS
                     GVFDIDMRSQEGAKASPPTSRAGEDGSIAFDPAVSSPYSNEIRLIQVIRDRDTAGNDV
                     NISSMPPGRGESLRTTAGLGSDVEAGFSTDVLHQDFDNAGNPTTVTPQGAQKGLAYEG
                     GTPIYGFKRSNDPRDIKSARIEDTPYSTGDWDFDFETVAKGEDTGVIYGSLHWSFGTR
                     HGRVVNEKVDAEDAQSATFDAAVEKHRDFYVHEPVVFYFDYKKDELSTTEAAKIDEFL
                     DYLHRFPDVRLSLDAYADNKGDALFNLHLSEKRGDNVIKALIAKKVDPARISKAVSSW
                     GSTEEFTSGDLAPNPGRSQNLEANRRGNRRVVLTFSHTQSLPAP"
     gene            complement(12116..13864)
                     /locus_tag="VGQ51_07800"
     CDS             complement(12116..13864)
                     /locus_tag="VGQ51_07800"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEV9036506.1"
                     /translation="MNHHIQKQQFILTVRQGIDAFAAQHAASRWLRQGLLPVLEKIFD
                     ELSGEDEVIRLDHCIIDLGVIPESVLQSGVVDEVMYRQLKQEFKMAIEKELSLRPGAI
                     TRAAEGVMQRWWYYMEHGRLPWNADSLGEEDYRRVLELFSVDFVQITRLRNALVEGST
                     FLTRVVAQHNGRFLENLVTVLVSSRQEGLAESVIRATEAHRILEGVYRLRAAAVGGKH
                     TVAAAFETSQHRDVPESLRRWASQMTTFRQAPVYEQKAIIWRRLLVFAVQHRDRFVRA
                     GGVQVLLQDIPEDLMEILVAAPEFRGGDGSFLQAMGRAAERRREKLDADLRPAGEAPG
                     SPADEKHGPSIYSADEAVSAKQTPAGETSAPENKYGDHGQEEGPAEPRPGEKSSQQGD
                     HVPAQAAKRPMSDEDQFRREQVTEEGIYLSNAGLILLHPFLSTLFNRLHFWDGGGFTS
                     LEARQKAIFLLHFLATDERTAPEYSLVFPKMLCGYTLEMPLPAEMTLSDEECAEALLL
                     LENVVLRWEKLGSTSPQGLREGFLQRRGKLFDKGGRLALLMESSAVDVLLDYLPWNMS
                     LVKLPWLKDILYVEWR"
     gene            complement(13861..>15076)
                     /locus_tag="VGQ51_07805"
     CDS             complement(13861..>15076)
                     /locus_tag="VGQ51_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036507.1"
                     /translation="IARNPQFYSYPAMAVDSVGRARERIDSEGLDIVEHLLLRPERSH
                     KEIPVCDELGPCSSILPFKPGSDPYSFILTIVLPAWPARFRKTENRVLLESILQREMP
                     AHILARILWLTPKDMCRFEHLYAGWIDGLAREATDREACGGFRTDEFFHLLFHHSMAC
                     PGDCRECDEGPQETESSGEGEWLSQTNRLYCWKDRECAEKWEPEQPQLPDEEPRPAGE
                     IIEVIVEVEPEREMPPPEREMPPPEREGPKREARPSDARMMRKLQSARRTRYEQKIAD
                     WEAFYGEEKPAGNVRAFLLDPLPSTRRLEEILKEILKKTGKRSAKTARQQELAAIILS
                     IYLDRIIMEANEEARWEHLRTTIQKSGIRIEDPAQFYHGWNPDEMRQLAPDLQEEMLQ
                     SLIREAAQNPKP"
BASE COUNT         2865 a         4354 c         4138 g         3719 t
ORIGIN      
        1 catgatccgc ccagaacagc ttttgaaacc ggatatctcg ttcccaattg acccctccga
       61 gaagaaagac ggccgtctga ccggtaggga tgaagccccg gaagttcttg cccctcgtcc
      121 cgcctatcgg ctgaaaatca ccttcgcctt tcagggcgac gtggatggag tagtcgaaaa
      181 gatccggctg tacatggtcc acgagggcga tgagaagcag acgagcgtca tcgtcggtga
      241 gttttggcgt attcgatttg ttgtaatcga ggatgaactg gccgatagga agctgccaat
      301 gctcgatctt gggcatgggg ggcggcgcat gatgcagatg cgggtgcagg acctgtccga
      361 tacgataccg gatgagttca tcgagatact tcaactcgat cggcaatggc ccgggcgagg
      421 tgatgacggg caccgaacga acctcagggg cgtcttcgcc ggcggcccgt tcgtcgtcgt
      481 tatcgttatc gccgtcatcc gtcgaagcat cgtccgtgtc atcggccgaa gcttcatctg
      541 cgtcatcggt cggggcgtca tcggcccggg catcatcggc cgaggcatca tccgggtcat
      601 cggccgaggc atcatcgtcg tcctcggacc ttgagccgac ctgggatttt ccttcttcct
      661 cttcggatac catagccgta agcgcgacat ctccgctggc tgccagtgcg gcccggggaa
      721 cccggcgacg tttccacagc caatcgaaaa gccaattcat ggtaaatgac ataaaaaagg
      781 tttaactggt ttgttgaatt acatgtgtca gctcgtgcgc cagaaggcgt ttgccttcct
      841 cactttccgg gttgtatttt ccttcattga aatagatatc gcatccatgg gtgaaggcct
      901 gggcgttgag ctcattgcac agggctgccg ccgtcgcgtc atgatgcacg cggacatgac
      961 tgaagtccgt tccgaacgaa tggttcattt cggcaagcgt cttcgccgga agggcactgc
     1021 ctttaccgga ttgatgctcc agctcggctt cgacggcgtg catgctggag tggccctctg
     1081 cctcatgggt cttctctttt ttatggatca tacctccgcc gcccggcgct tttttctttt
     1141 tcttctccgg atcttcagct ctgcggatat gctttttctt ccttttgtcc atgttgtgcc
     1201 ccttcttctg gaccggcttt ttctttttct tcttgtcgcc gggagcctgt ttggtctgca
     1261 cgggcttcat gggctcttcc ttgtccttct ccatacgggc gtcgttggtc cccagctttt
     1321 catcctcttt ggaggttgcc agccgctgca cgaagaaccc gctctttttt ttccgttgta
     1381 cggcggcgcc ttttccggtc tggaagaaag gtccgctgtt ggttttctgc tcgtcccccg
     1441 ccttggggtt gcggtgacgg taattcctta gactatatga catgatcgta ggattttatg
     1501 atcaattatt cttttccaat ttttttaaca gctcatccgc aaggccctga tagtccttcg
     1561 cgccgttaga gcttttgtca taggaaaaaa tgtcttttcc gacctcctgc gccttcacca
     1621 gctggatatt ggtccggata actccttcga ataccttacc ggggaatttc ccctccagct
     1681 gctgcttcac gctaagattc atcaacttgc gatcgtcgta tttcgtcagc acgatgccca
     1741 gcagctgcag ctgtacattc agcttcctgc ggatcatatc gaactgtcgc atgaagctat
     1801 agacgccttt cagcggcagg aactcaccgg gcaacgggat cagcaccagg tcggaggcta
     1861 ccagcgcatt caccgtcagc attccgatag catgtggaca atcgatcaga actatatcat
     1921 attcctgcag cagcggctgc agaaggcctt tgagcagctg ttccctccca tatacactga
     1981 ccagctcgag ctcggcgatc gccagatcta tcgatgcagg aaccacatcc gggcccgcca
     2041 cgctgtgaac gatggtttgc cggaggtctc cgccatctcc ctgtatttcc tttttcagct
     2101 cggtatacag attatagaca ggctcatcct cgatacccaa tgcctgcgac aggttggcct
     2161 gcggatcggc atccaccact aatacttttt tacccgcccg cgtcagggcg gcgcctaagt
     2221 tgacggcagt ggtggtcttg ccggacccgc ctttttggat ggcgatggag atgacggaca
     2281 tgctattttt tttaaacgtt gattccgaat gatgcgttat tcgaccggat atgccctttc
     2341 cagcttctga tcccatctac cacgaggtct atctgattga aatactcggc gaatacccga
     2401 taagtgccgg gctcttcata ggaaaaatgt tcttcaaggg tgtacaccga atacagatac
     2461 tctcccggga acaaccggac aaaatccccc ggaaggggag gtttggcctg aacatgcacc
     2521 ctcatcaggt tcatactgtc gggcggccca tcgatatgga tgaccatttc cttcctgtcg
     2581 tcaggcatcg ctgccggtac gagcaacatc cgggaattga ccagcagaat atcatcgcct
     2641 tcattgataa gacgggcggt cagttccggc atttctccgg cgcttacggt cggggtattc
     2701 agtgtgatga gcagcgtcag catagggttc gtgcgttgtt ggttgactcc tttgcaagga
     2761 aataataggg tgtaccggta aggacgatcc cgtcgatggc tatcttctta tcggcgaatt
     2821 caagggttag acaggggatc ttgccttcat agggatgatg gaagatatca ttactgatgg
     2881 tataccccgt tgcgtattcg ctgtcgccgc gggacaggat gacgatctgc cggctcgtct
     2941 cgagcctgta gcggaaatta agaagaagcg tcgtctcacc tgctttgtct tcttcataaa
     3001 caccttcgcc atttgacagg aaagaaagac ggtcacggtc gggccaggcc aggggatccc
     3061 gcatccatgt ccgggtgatc gaatgatcat ccttcacggg cgccggaggg ggactcttta
     3121 aaaattcttc ggcccaatgc cggacatccg gtgagcggtc ggtgctcgcc ctgatgaaga
     3181 gcggatggga cccctcatgg tcgatcttgt tcaacgcgat gaggatggct atcttcgtat
     3241 cggcatcttt ctcgccgaag agtgcgctca gcggtgaaat ggccgcttcc ccaccgatct
     3301 ctccaagaga gaagatagcc atctttttca acagcgggtc ttcattgacg ttgtcgatca
     3361 gcgccagcag gaatgggatg gcggtctttt ccctggcttc gccccatttt ttgacgatgg
     3421 tcctcctgat atcctggtcg gcggtcgata acaatccttc caatgcaggc ccgaaccgga
     3481 cggcgtcgga aggggataat gatctcaaac ccgcgcgcag caattccctg tcggagctgg
     3541 ccagcacttc tctgatatac gggtccctgt cttctctttg catcaggagc ccgatcaggt
     3601 aactgcgcag gttttcgggc cccgtattgg cggcgtcgta caactgtctg ctactgtatc
     3661 gttcatagtt gttcatcggt gtctcatctg aatctcaaaa ataaccactt ccgcaggaag
     3721 cgcagcttct tgacggccgg atcgttcaat acgtcgacgc ctttgctcgt gaaggcgacc
     3781 tggtggatgt tcggcttttt ggcgaggctt ccccacagcg ggccttcgat gacgtcgaac
     3841 cccgcgcgag ctccccagtc cggcaggttc cctttccggt tctcgcgttt gttatactcg
     3901 acgaaatcct tccagtcttt gaatggtttt ccggtatcgg gattgatcgg catattggca
     3961 ggtttgctcc tggtgttcct gaaatagagc tcactgtgga tatatgctgt ccagaaggag
     4021 tccggtatgg ggaaggtgat gactccccag ccgcgttgac ccagcgaccc gcggatcgac
     4081 caatcgcggg ccaggcgcca gttgcctttg gtatgggtat agaatgcgcc gccgaaatcc
     4141 acaggcgaga cgcctttgat gtctacagtg cggagctttt cgacgtcgtc atagttggtg
     4201 ccgtgatggt agacatggga gtagccgggc atcgggggcg gcggcgccac gaggtccttt
     4261 acgaattgct gtgccttatt gtaccggacc tcataaattt tttgacgggc ccggctgaag
     4321 ccgccgatgc catcatagtg atccgtaaga cggcgggcta tgtcgagcat gtcacggtcc
     4381 ctgcttgcga gacccgtttg atcggagttc tctatctccc acagatacgc ttccgtttcg
     4441 gcaacctcca gttcctctcc ggcatgacct ccgtgcgcct cgtcgatgaa tttggcgggg
     4501 tcctgaccgg gcacctggtc ctggaggaca tgctggtact cgtgcatcac ggtggacacg
     4561 agctcctgga tgcttccgaa tgccggcgta tagatggtca cacgggaagg accgctggga
     4621 tccattttcc ccgttgccgg atttttgtgg aatttcgtgt tcgtcaaccc ttcaccctta
     4681 tcggtccgat tcacgaatct gatcgtgaat ttggcggggt cgacatgctt attccgttcg
     4741 aggtctttca tcagcacatc cagcgcgtcg gcataccgct tgctccgcat caggcgctga
     4801 acgtcgttga acgtctttgt ttcgatcatc ggcacgtccc tgctgaacgc ggcggtcccc
     4861 gtcaaccgga tcgaaacatg gggcgagcgc cagtcgatgc ccgaccagcc ggtatcgtca
     4921 ttgcaacaga aacatccgaa gatgctgatg gtgacgcttt tccccgttgc tatttccgtt
     4981 gcggtgcaat gccaggtctc acccggaacg tcttcaccac agacttcccg cggggcgcca
     5041 ctcttgtaag gcgggaactt gtcgaatttt ttacaatcgc ggacgtcttt atatccattc
     5101 tttgccgccc attccgacaa taccctatcc ctgtcggaac cgtcttccgt gcaatcggta
     5161 aaatccggat tggcgtccct gcattcgttc tgggcgagga ccccgctcaa cggctcggct
     5221 ttatccgcga aatagagctt gccatcgaat ggcggaagac cgaatgccca ggcgatgcag
     5281 ttacctgcga gattgttaca ggtacattcg atcgtggctg ccatatgcca gggcatgaac
     5341 atggcccctt ttccagtctc ccccttttcc gctgctgccc agtcgaaagc cgcgtcatgc
     5401 tcgcggcaac cgccgttggt gtcgcaatcc tggaaattcg tatacagcca gacttcgccg
     5461 gtgaccgggt cggtaaaggt gtagctcgga taaacctcgc aggtatcgca attcacgccg
     5521 cacgcgcccc tgcacgaggc tccactgacg attttgggat cgcgctggta tacgttcagc
     5581 ggcccggcca gtcgcggccc cggtccgtat ttgcctccat gcccggtacc cgtcgggtcc
     5641 catactccgc cgttctgtga aggcagcagc ttcttatccc gaaggtactc gcatagcatg
     5701 tcgagcagcg ggcaatcgtc ggaaaagcct tcgcgcgaga tcgccagcgg tagctggtcg
     5761 aaggggatct cgtccatctt cggcttgatg acatgacagc cgccgacgcc cggcagggcg
     5821 cccgtgccca cgtgcactcc acccgagaga tcgaaggaat aggcgatgcc gccatgccat
     5881 tcatatggct gccaatagat cctgcacagg tatttatcca gcagcttcaa ctgcgcatag
     5941 gcgccggcga aaagatcggc gccgagaccc agctcgagtc tgccaaaccc gccggctgtg
     6001 atcgtgccgc cgctatagtc gaggctgccg ccaagggtca ggcgtcccac tccgatgcca
     6061 cgtaccagac cggcaagacc gccttcagct tctatcaacg gaaaatctac ggggatgggg
     6121 attgctccga caaacaccac tccgttgagg cgaagctcgc cctgtacgcc tcctctgatt
     6181 tcagcaccga gcgaagccgc tgcagccatg ctgatcgctc cgctgacgct gtaatggttg
     6241 gacaacggat cgatgaggat ccttgcgcct tccagacaga agggaccaag ctgcgtctgt
     6301 atctccggcg tcaggctgcc tttcaatcct atgcttcccg tgatgaaaag accgggcagt
     6361 atctcgaaat tgacgatggg caccaggaat cctttttccc agggatcgag gacagatctg
     6421 tcggtaatgg cgttgatcgg gcaggtccgg ggcagaatga tctcgagacc gccggtggcg
     6481 gtgatatgta tatcgacgcc gaagaaatcg gccaatgcgg atgcggcctg cccggcttcg
     6541 ctctgaagcc agtccatcgc ttgcgaaccc gcgctggcta tttgctgtac ggctccgctt
     6601 ccggcgttat ataaggcctg acccgtatct tttgcacctt cgaccaccgc ctgccccgtg
     6661 tgatacaagg cttcaccggt gtcttccgca gccccggcaa tggcatgtcc tgcatcggac
     6721 agtccctgac ccgccgcatc cacgccggcg gatacatccg atttgaaatc gtcccagaga
     6781 ttgcggcgaa gggtatctcc gttctgttgg acgacatgcg tcagctcatg tgccagcagc
     6841 ttcttgcctt ccttgctccg gtcgtcgtat tttcccgaat tgaaatagat gtcgctgccg
     6901 tgagtgaagg catgcgcgtt gagctcattg ttcatggcgg cggccgagct atcggtgtgt
     6961 atccggacac cgctgaagtc ggcgccgaag gcctggctca tcgagctgtt ggtatcgtga
     7021 ctcatcgggc ttcctccgcc acggtggctg ctgagactgc tttcgatgga gggactcgcc
     7081 gccgcgccat cggcgccttt tttttgcagg tgcttctctt cttcttcgca gtgtttgcac
     7141 ttgcgctgaa ccttcttctc ttcttcttcg cagtgtttgc acttgcgttg gatggggtgg
     7201 tcgtcttcgg gagggtcttc attactttgg aagatcggtt tcttgcggat ctttcctttc
     7261 tcctccccgc ctgtctcttc ttcctttcta tccagcttgt cttccttatc gcaagcggca
     7321 cattcctgta tcggggccgc attggctccg gcgggcataa agggtgtcac cgcaggcgca
     7381 ggggccggcg ccggttcatg actttcaccg ccccggctca gccgttggac gactttgtcg
     7441 gctaccgaat cggcttcttt ttcatagacg tcatgtggtt gtccgatggt cagcttggtc
     7501 tgtacgatgc cggagggtgc cataccgccg gatcccgccg cgccgccgga gaagaatcct
     7561 cctgaccgcg aaggctgaaa aaaaccgttc cccctgctct tttgaaagaa ggggcccgac
     7621 ttattagccg cggaagtaga cctatgtgat tccatcgttt tcatgttctg ttctttagcg
     7681 ctcgtgcttt cttcagggca agggtcggat atcatcgatc gtatagtgac ggatcaccat
     7741 attggggaat ctttttatcg ccgtcccgat agggatctcg atcgcacagg gtctggccgg
     7801 gtcggggcgg aaatagatcg gttgcgcatt gttctggatg gagccggccc gctcattata
     7861 ggccgattcc aaaggccaga ggttggcaaa atcatccgga ccctcccaaa agaggtcctg
     7921 gacatgatcg ggcgataggt tgtctccagc cgagttcttc cagctgaatc caagactggc
     7981 caataccctt ttgaatctcc tttgttgcgt cctgcgtttg ttgttcttgt ctatttgcag
     8041 tgccttgtga tgtgccggca ggaatggacc cagcacaccg atggtatcgg tctgatgagg
     8101 cggcggcagc ggcttacgag acgtcatagt gaaagtcgtt ccctttagaa cgatggggtt
     8161 ctcatattga ttcagcggtt tgaaccaaat gatgtctatg gggtctgccg gcgtcatacc
     8221 ggtaggagcc gtcggatcgg ggaactcgtc gtcgttgcaa tcgttttcat ccggatcgtc
     8281 cggatctggc tcgggcgcgg gttcgggctc cggtatgggt ataggttcgg gtgtgggcac
     8341 cggcttgagc agctcttcga tcaatagata caggagatag accaccagcg caatgatcac
     8401 aagaatgagg acaatgatca gaatgatcaa aaccacttcc catccatcga ccacgacctc
     8461 tgccgcggtc tccgcctcct cggcgacaag ggtggcttct tccaccaccg cgagctcttc
     8521 tcccgccaat tttaaggtct gatcgaacag cggctccagc tcctccagct ccgtgatcaa
     8581 ttcttcccac tccggggtcg tttgcggtgc gggtaaattc gccaatgcac tgcctccgcc
     8641 ccccgtgaat gccgcagcga cgtcggcccc ggtcccgcct ccggagacaa actggaccac
     8701 cagccggccg atgtaggagc cgatggcgct cactgaggag gttagtattt tgacgatcgc
     8761 atcttttact ttccccacca gcaagataaa cttggacacg agctccaatg cattgtccac
     8821 ggccttgctg aacaatgtct tgatcttgtc gatctcgccg gatatgaagt cccccacttt
     8881 ttggatcgcg ctgtttacca tcgaactgat gttctcatcg aattgacgga gagcagtctg
     8941 ccacgcatga tagacaaact cgctaaaggc gaggatctgg tggatcgcga tcatgaagaa
     9001 tgagctggtc tccgccacaa aatttgcaac gatctcggca ttgcgccgga cgccaacctg
     9061 ttctacctgg gtgaagtaga gcaggcgctg ttcacggctt gtcgtcgccg cgcctttctt
     9121 ctgtggtttg gttttcggat cgatcacgtc tgccatggct ttcaacacct gcgcctcgtt
     9181 gtcatccagc tgcatcagtc ttatcaaacg tgcctcctcg acctgttcga ccaaaaagtc
     9241 ttcaccctcc tgaaggatcg gccagagagt gccctggaga aagctgttca tttgcgaaag
     9301 gatcatgttc ccaacgcgct gcacagccat gaacagggtt tgttgaatgc ccaggatgac
     9361 ttcgaagatc ttattcacca attgccggat gagttgaccc gccgtccgga tcacatcggt
     9421 tatcatttgc gacgccttgg tcaatgcacc ttgcagggca ctagtgacga gggagatcac
     9481 tcccctggcg gcagatgccg cctggttcag gatgctaacg atcatatccc gtaccattcc
     9541 gacgcccgga agatcgggaa ggtgtacgga ggagatgagc ccggtgatcg ttccggcgat
     9601 atcattgatg gaatccctga caccgttgct gatcccatcg atgaaagcgg acaattgatc
     9661 gcggaacagg tctccgagct tgatggtgcc ttgttccgca tccgtgacga aggcctggac
     9721 cctgttcatg gcattgaagc tcatctgcat gaactggatc tgtttttgct ggatggtgtt
     9781 caggacgtcc tgtccggttc ctataaagaa ctggttgatc tccattcttc ttccgtcgaa
     9841 ccattgtctg atctccttcg tccgcgccac ggcgatctgg tcgatgagcg cgcggctcag
     9901 cactacgtcc tgttccaggt gggccgcttg ccggaccgcg gcaatcttgt acacactgtt
     9961 atgcgcatgc gtcagtctcc ccatctgctc tgatgggagg tcgccggttt ttggctcttg
    10021 cccggtgacc ggtccattgg gggcggcctg agttgctggt ccactcgttg ctgtctgagt
    10081 cgccggtccg cccgcagttg ccttaccctg cgcggcagac ggtgtgccgg ctgccgtttt
    10141 tgcagttgct gtttgcggta tcgcagacgc cgccccaccc gcggcatttg gggccgcctg
    10201 cgtggactcg ggttggacgt ttgtccccgg caaattttgt ccgccaatgt cctggtccgg
    10261 gacggccgga gccgttttta cagcggcgcc gccctgctgt acgacatgcg tcagctcatg
    10321 tgccagcagg cgttgtccgg ccttgctgcc ggtatcgaat ttccctttgc cgaaataaat
    10381 gtcattcttg tgagtgaagg cctgcgcatt cagctgctca ttcatttgtt gtgcctggga
    10441 accggtatgt atgcgtacgc cggaaaaatc tgcgcccatg gtctgttcca tgggggcccg
    10501 gtcggcagac gacaatgccg aacccccgcc atggctggcg tgcaactgtt gctcggtatg
    10561 agggctggcc gtctgattgg cgcccgcgtt ttcttttttc tgtaatttct cttctttttc
    10621 gcaggccgcg catttacgtt gtatcccttc ttctttcctc tccgctttgg attcaaagat
    10681 ggggcggctc atccgctgaa cgaccttgtc ggctacctgg tccgcctccc gctcgtacac
    10741 atctcccggc tggccgacgg tcagcttggt ctggaccgga cccgcctggg agaaaaaagc
    10801 cccgccggcg ttgccctttc ggccgcgctg atgtcccgaa ggtgaaaaga agccttgccc
    10861 cctttcttta ctaaagaagg gggagaccgt tttggcggaa gaggccggta tttttgaaac
    10921 tgccgttttc aaacaaggta ttttttattg ttgcgtcagg atacgggtaa tcagggcgcc
    10981 ggtaacgatt gagtatgcga aaaggtcaac acgaccctgc gattgcccct tctgttggct
    11041 tcaaggttct ggcttcggcc tggattaggc gcaagatcac cgctggtaaa ttcctccgtg
    11101 ctgccccagg acgaaacagc tttagagatc cttgccgggt cgactttttt cgcgatcaat
    11161 gccttgatga cgttgtctcc tcgtttttcg gagaggtgca aattgaaaag cgcgtccccc
    11221 ttattatcgg cgtaggcgtc cagagacaga cgaacatccg ggaaccgatg cagataatcc
    11281 aggaactcat cgatcttggc cgcctccgtc gtgctgagct cgtctttttt atagtcgaaa
    11341 tagaatacga ccggttcgtg tacataaaaa tcgcggtgtt tctccacggc tgcatcgaag
    11401 gtggcggact gggcatcctc cgcatccacc ttttcattta ccacgcgtcc gtgcctggta
    11461 ccgaaagacc agtgaagact gccgtatatg acacccgtat cctcgccttt tgctactgtt
    11521 tcaaaatcga aatcccagtc acccgtagaa taaggtgtat cttctattct ggcggatttg
    11581 atatctctgg gatcgttgga tcgcttaaag ccataaatgg gcgttcctcc ttcatacgcc
    11641 agcccctttt gagcgccttg aggagtaact gtcgtagggt ttcccgcatt gtcaaagtcc
    11701 tggtggagga catccgtaga aaatccggcc tccacgtccg agccgagacc cgccgttgtc
    11761 cgcagggatt ctccccgacc gggcggcatg gaactaatat tgacatcgtt tccggcagta
    11821 tccctgtcgc ggatcacctg gatcaatcgg atctcatttg aatatggaga tgacacggcc
    11881 ggatcgaacg cgatggaacc gtcttcgccg gcacgcgacg tgggtggcga ggccttagct
    11941 ccttcctggg aacgcatatc tatgtcgaat actccggagg caaccggtat cgactgcccg
    12001 atgtcgcgct ggatcatcgg tgccggtgct gccgaaaaaa agccctgtcc tccgaagaaa
    12061 ggaggtttct ctttagcctg aagggctggc tctttgggtt tgtgcattct catcgttatc
    12121 tccactctac atataaaata tcttttagcc acggtagttt cacgaggctc atattccatg
    12181 gcaggtaatc cagcaggaca tcgacggccg aagactccat caacagcgcc agccgcccgc
    12241 ccttatcgaa cagcttgccg cggcgttgca gaaaaccctc gcgtaatccc tgcggtgagg
    12301 tgctacccag cttttcccaa cgcaggacta cattttccag cagcagaagg gcttcggcgc
    12361 attcctcgtc gctcagcgtc atctccgccg gcaacggcat ttccagggtg taaccgcata
    12421 gcattttcgg gaagacgagg gagtattcag gcgcggtccg ctcatcggtg gcgaggaagt
    12481 gcagaaggaa gatggctttc tgacgtgctt cgagactggt aaagccaccc ccgtcccaga
    12541 aatggagacg attgaacaac gtgctgagaa aagggtgcag aaggatcagg ccggcattcg
    12601 aaagatagat gccttcttcg gtgacctgtt cacgtcggaa ctggtcctcg tcggacatcg
    12661 ggcgttttgc tgcctgggca gggacgtggt cgccttgctg tgaagacttt tcaccgggcc
    12721 gcggttcggc cggaccttct tcctggccgt gatcgccata cttgttttcg ggcgcagacg
    12781 tctcacccgc aggagtttgt ttggcggaga cggcttcatc cgcggagtaa atggatggtc
    12841 cgtgcttttc atccgccggg gatcctggtg cttcgcctgc gggacgaaga tccgcatcga
    12901 gcttttcgcg gcgcctttct gccgcgcgcc ccatcgcctg caggaaagag ccgtcgccgc
    12961 cccggaattc gggggcggca accaggattt ccatcagatc ttctggtata tcctgcagca
    13021 ggacctgcac accgcccgcc cggacaaatc tatcccggtg ctgcaccgca aaaaccaaca
    13081 gccgtcgcca aatgatcgct ttttgttcat agaccggcgc ctgccggaag gttgtcattt
    13141 ggctggccca ccgcctgagg ctctccggga catcccggtg ctgcgacgtt tcgaatgccg
    13201 cggctactgt gtgttttccc ccaactgcgg ctgcacgtag ccggtatacc ccttccagta
    13261 ttcgatgcgc ttcggtcgcc cggatgaccg attcggccag tccttcctgt cgcgatgaga
    13321 ccagcacggt cacgaggttt tccaaaaaac gtccgttgtg ttgcgcgacc acccgcgtca
    13381 ggaaggtcga accctccacc aatgcgtttc ggagtcgggt tatctgtaca aaatcgacag
    13441 agaacaactc cagcaccctc cggtaatcct cctctccgag actgtccgca ttccagggaa
    13501 gccggccgtg ctccatataa taccaccagc gctgcatgac gccttcggcg gcgcgggtga
    13561 tcgcgccggg tctcagcgac aattcttttt caatcgccat tttgaactcc tgtttcagct
    13621 gccggtacat gacctcgtct acgaccccgc tttgcaggac ggattccggg atgacaccca
    13681 ggtcgatgat acaatgatcc agacgaatga cctcatcctc ccccgacagc tcgtcgaata
    13741 tcttctccag gactggcaac agtccctgcc gtagccaacg gctcgcggca tgctgagcgg
    13801 cgaaggcgtc tatgccctgt cgaacggtca ggatgaactg ctgtttttgg atatggtggt
    13861 tcatggcttc ggattttgcg cggcctcgcg tattagcgat tgtagcattt cttcctggag
    13921 atcgggcgcc agctgccgca tttcgtcggg attccacccg tggtagaact gcgccgggtc
    13981 ctctatccgg atgccggatt tttggatggt ggtccgcagg tgctcccaac gcgcttcttc
    14041 gttggcctcc atgatgatcc ggtcgagata gatggaaaga atgatggcgg ccagctcctg
    14101 ctggcgggca gttttggcgc tgcgttttcc ggtttttttc aaaatttcct ttaatatttc
    14161 ctccagccgc cgggtggacg gaagcgggtc cagcaggaag gcgcggacat tcccggcggg
    14221 cttttcttcc ccgtaaaatg cctcccaatc cgcgatcttt tgttcatacc gtgtacgacg
    14281 ggcggactgc agcttgcgca tcatgcgtgc gtcggaagga cgcgcttcgc gtttggggcc
    14341 ttcgcgctcg ggcggaggca tttcccgctc gggcggaggc atttcgcgct ccggctctac
    14401 ttccacgatc acctctatga tctccccggc cggacgcggc tcttcatccg gcagctgcgg
    14461 ctgctcaggt tcccatttct ccgcacactc gcggtctttc cagcaataca atcgatttgt
    14521 ctggctcaac cactctcctt cgccggacga ctcggtttcc tgcggccctt catcgcactc
    14581 ccggcaatcg ccgggacatg ccatggaatg atggaagagc aaatggaaaa attcgtcggt
    14641 tctgaaacct ccgcaagctt ctctgtcggt ggcctcacgg gctagaccat cgatccatcc
    14701 ggcatacaga tgttcgaacc ggcacatgtc cttcggggtt agccagagga tacgggccag
    14761 gatatgcgca ggcatctcac gctgaaggat gctttccagc aaaacgcggt tctcggtttt
    14821 gcggaacctc gccggccagg cgggaaggac gatggtcaat ataaaggaat aggggtctga
    14881 tccgggtttg aagggtagga tcgatgaaca cggccccaac tcatcgcaca ccggtatttc
    14941 cttatggctg cgctccggtc gcaacaacag atgttcaacg atgtctaacc cctccgaatc
    15001 gatacgctcc ctggcacgac ccaccgaatc cacggccatc gccggatagg aatagaattg
    15061 cggattacgc gcgatg
//
LOCUS       DASYSV010000254         5828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1709791, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000254 DASYSV010000000
VERSION     DASYSV010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709791"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VGQ51_07810"
     CDS             complement(<1..1421)
                     /locus_tag="VGQ51_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEV9036508.1"
                     /translation="MTSLIMPIQRKIAWLMLLSFLSLCFIGWLIYFNQKNADSSRWWI
                     SHTHAVILSVEKINTRLSAWDDLRQPAMPAAYRQGLYRDMVEVKSLTIDNVNEQSLIV
                     RLSGIIDSLASDPSRTRIAAARTILGRMIQEENDLLEQRRILNRVTNAKSIWTLIGES
                     VLGFLLINIILLRLNKDMFLRRKAERQLQRNQTWLESILDNSTSLIYIKDPLGHYVMA
                     NRRFQEVLGVKAEDVIGRTDDDFSTADAAAHYRSLDEQVILTRKSLEIEEVIPVPGGE
                     VYLLSIKFPLLDSKGNLIGIGGIATDITERVQNHQQLIAATREAQSAKSMQELFLANM
                     SHEIRTPMNGIQGMSDLLLDTPLSEQQREFAKIIKRSVNNLLVIVNDVLDLSKIKAGK
                     LAIEKIEFRLKDVLENARVLFEHRIAKKGLQLLMEIDPAIPDTLKGDPYRLNQVLINL
                     IGNAIKFTEQGWVRVEVTAQERST"
     gene            1687..2691
                     /locus_tag="VGQ51_07815"
     CDS             1687..2691
                     /locus_tag="VGQ51_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036509.1"
                     /translation="MKTKHFQHLMMATLLSGLLFASCKKSNNNNSGGTSDQDLQTQSD
                     DETRVSTETDAAFDDVNTVMVGQAAVTGASETPALRYGVAVNGGNQDTVKSSICDAVV
                     TIDTIDNPRTITINYNGGNCLLTRVRTGSVVISIPKGTRWSTAGAAVTVNFNKLKIVR
                     VLDGKSITLNGTHTYTNVSGGSLLSLNANSTTPIVHSVTSDNMSITFDNGSQRTWSVA
                     RQRSYSYSGGIVITETGLHTSGNTTGISEWGTNRFGNSFQTQISQALVVAQSCSWQLT
                     TGQVVVTNSAGSTTVTFGLNSTGTPQTSCPIGTAAATYYFQLVWTGNAGKTYTLIWPY
                     "
     gene            2769..3668
                     /locus_tag="VGQ51_07820"
     CDS             2769..3668
                     /locus_tag="VGQ51_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036510.1"
                     /translation="MKFLLVMLFAAVLFSCSKKGSGFNDSSSFNANVEAQTNDQVRIS
                     TTIDAIFNDVDSVLANPVNLCEANVSVDSVDSPRSITIYYNGAGCGFLLYHQGTITIS
                     YPPGNDWTMPLDTVLVTLKNVVINTPPDTSGLVFNGSCYYTNVSGGSLSGLTSGGVSP
                     VIHSITGINISVAYNYYWPSTWQIARQRSYTNNGGLVISTIGMDSVGGYAGVAEWGGN
                     RFGNSVITAVDSPLTITQGCSWRLTSGKIQLYNPVGITSMIYGLDSTGVATGCPDGGS
                     PYYYKLAWTGSGQNPYTMVLPYP"
     gene            3754..5157
                     /locus_tag="VGQ51_07825"
     CDS             3754..5157
                     /locus_tag="VGQ51_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9036511.1"
                     /translation="MQTVTTDWLRSVDVLADVPSDQLQWLIDSSNCREIPEEEYVFRS
                     GDPVKGMYIVLNGKIRLFIVQQNEMREVGVLEPRDVSGALPFSRSVKATVHATALKDT
                     AIMMLPVEKFREMVSRHYELTAALVHVMTDRVRNFTTLQQQNDKMMALGKLSAGLAHE
                     LNNPAAAIVRGSESLKQHLQLEPESFKDVINVRMEAKDVDFLKDKLFAILARTDKPRL
                     TLVQRTELEDEMRDWLDEQKVQNSDEIAENFLDYAFTCDDMEQFHAHIPDQYLSPVLN
                     WINTNLVTERMVQDINEASQRIFRLIQSVKNFTHMDQGKGKELTDIHTGIGSTLTMMQ
                     YKLKKANIRVVEEFDPALPKIMALVGELNQVWTNLIDNAVDAMEVNGKGQLTIRTMKD
                     HSFAKISVIDDGPGIPAGIRSNIFDPFFTTKGIGKGTGLGLDVVNRIVQGQHRGSVKV
                     ESVPGRTEFIVCLPIAG"
     gene            5169..>5828
                     /locus_tag="VGQ51_07830"
     CDS             5169..>5828
                     /locus_tag="VGQ51_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9036512.1"
                     /translation="MALPIIFSIDDDPQVLRAITRDLRTQFRQSYRILSTTSVKEALE
                     SLLDLRNKGETVALFISDQRMPEMEGVDFLQKAREFYPDAKRVLLTAYSDTDAAIKAI
                     NSVQLDYYLMKPWDPPEEKLYPTINELLDDWQSGWRPAFRGIKLIGYQFSPKSHEIKE
                     FLSGNLVPYQWQDIESNQDAQQSLELNKIALKDLPTLFLEDGSFFCSPEIPLVAEKIG
                     LN"
BASE COUNT         1394 a         1647 c         1488 g         1299 t
ORIGIN      
        1 gtactccgct cctgtgccgt cacttccaca cgaacccatc cctgctccgt gaatttgatg
       61 gcgttgccga tcaggttgat cagcacctgg ttcaaccggt aagggtctcc tttcagcgta
      121 tccggtattg ccggatcgat ctccatcaac aattgcaggc cctttttggc gatgcgatgt
      181 tcgaaaagca cacgggcgtt ctccagcacg tccttcaatc ggaactctat tttctcgatg
      241 gccagcttcc ccgcctttat cttcgaaaga tcgaggacgt cgttgacgat gaccagcagg
      301 ttgttcaccg atcgcttgat gatctttgca aactcccgtt gttgttcgct cagcggcgta
      361 tccaacagaa ggtcggacat cccctggata ccgttcatag gggtacggat ctcatggctc
      421 atattggcaa ggaacagctc ctgcatgctc tttgcactct gcgcttcacg ggtggctgct
      481 atcagttgct gatgattttg caccctttcg gtgatgtcgg tggcgatgcc cccgatgccg
      541 atcagattgc ccttgctatc cagcaaaggg aatttaatgg agagcaggta tacctcaccg
      601 cccgggacgg ggatcacctc ttcgatctcc agcgatttcc gcgtcaggat cacctgttcg
      661 tccagcgagc ggtaatgcgc agccgcatct gccgttgaaa aatcatcgtc ggtgcgaccg
      721 atgacatcct ccgccttaac cccgagcacc tcctggaacc ggcggttagc catgacataa
      781 tgacccagcg ggtctttgat atagatgagc gaggtcgagt tgtccaggat ggattccagc
      841 cacgtctggt tccgctgcag ctgacgttcc gccttccggc gtaagaacat atccttgtta
      901 agccgcagca ggatgatatt gatcagcaga aaacccagca cgctttcgcc gatgagggtc
      961 cagatgctct tcgcattcgt cacccggttg agaatgcgcc gctgctcgag caggtcgttc
     1021 tcttcctgta tcatcctgcc aaggatcgtc ctggcggcgg ctatcctggt tcggctgggg
     1081 tcactggcca aagagtcgat gatgcccgac aaccgaacga tcagcgattg ttcgttgacg
     1141 ttatcgatag tgaggctttt tacttccacc atgtcccggt agagcccctg ccggtaagcg
     1201 gcgggcatcg ccggttgccg caagtcatcc caggccgaaa gccgggtatt gatcttttcg
     1261 acggacagga tgaccgcatg cgtatggctg atccaccacc tggaagaatc agcgttcttt
     1321 tgattgaaat agatgagcca gccgatgaaa cagagcgaca ggaatgataa tagcattaac
     1381 caggctatct ttctttgtat gggcatgatc aatgacgtca tatgcgtttg gctggagccg
     1441 aaaaccacgg attgacgata cgtgaaacgg ataaatcggt atttggtata ggagggcggg
     1501 agacgaacaa aagaaaacgc attagcattc agggtttacc atagttttcc ctgctaaata
     1561 acgaaaagaa aacggttttc tgatatgccg gaaaaaataa tatatccttt cccccgttaa
     1621 actatacgat gtgccgctgg tcctatgtag cacctttttg aaaactgaaa aaacctacta
     1681 aatcaaatga aaactaagca tttccaacat cttatgatgg ccactctgct ctccggcctg
     1741 ttgtttgcct cctgtaagaa gagcaataac aataactctg gcggcacctc agaccaggac
     1801 cttcaaacgc aatccgatga tgagacccgt gtttcgacag agaccgatgc ggctttcgat
     1861 gatgtcaaca cggttatggt cggccaggct gccgttaccg gcgccagcga gaccccggct
     1921 ctccggtatg gcgtagccgt aaatggcggc aaccaggaca cggtaaagag ctctatctgc
     1981 gatgccgtcg tcaccatcga taccatagac aatccccgca ccattacgat aaactataat
     2041 ggcggcaact gtcttctcac ccgcgtacgt accggttcgg tcgtcatttc cattcccaaa
     2101 ggcacccgct ggtccacggc aggcgcggct gtcacagtaa atttcaataa actcaagatc
     2161 gtccgtgtcc tcgacgggaa aagcattacc ctcaacggca cgcataccta tacgaatgtc
     2221 agcggcggca gcctgctgag cctgaatgcc aacagcacca cgcccatcgt tcattcggtc
     2281 accagcgata atatgtctat cactttcgac aacgggtccc aacgcacatg gtctgtcgcg
     2341 cgtcagcgct cctattcata tagcggcggc atcgtgatca ctgaaacagg tttacatacc
     2401 agcggtaaca ccaccggtat ctcagaatgg ggtaccaacc gttttggcaa tagcttccag
     2461 acccagatct cgcaggccct ggtagtggcc caaagctgta gctggcaact gacgaccgga
     2521 caggtggtcg tgaccaattc tgccggttcc accacggtaa ccttcggtct caactccacg
     2581 ggcactccgc agacaagctg tcccatcggt acggctgcag ccacctacta cttccaactg
     2641 gtgtggacgg gcaacgcagg aaagacctac acgctcatct ggccatatta acataacctt
     2701 tcaaccaatg aatgagcggc cgacctgcat ttagctattg ggaggccgct tttttgtatt
     2761 ttcgtcgaat gaaattcctg ttggtcatgc ttttcgccgc ggtcctgttc tcctgttcaa
     2821 aaaagggttc cggtttcaac gattcatcct ccttcaacgc caatgtggag gcccagacca
     2881 acgaccaggt gcgtatctcc accaccatcg acgccatctt caatgacgtg gacagcgtcc
     2941 tggcaaatcc ggtgaatctt tgcgaagcca acgtctccgt cgattcggtc gacagtccac
     3001 ggagcataac catctattac aatggtgccg gctgcggctt tctcctctat catcagggta
     3061 cgatcaccat cagctatcca cccggcaacg actggaccat gccgctggac acggtactgg
     3121 tgaccttgaa aaatgtggtc atcaatactc cgccggatac atcgggtctc gttttcaacg
     3181 gtagctgtta ctatacaaat gtcagcggcg gcagcctgtc cggtctcacc agcggaggcg
     3241 tctcccccgt catccattcg ataacgggta tcaacatcag cgtcgcctac aattattact
     3301 ggccctcgac ctggcagatc gcccgtcagc gatcctacac caataacggc ggtctcgtca
     3361 tctccactat cggaatggat tctgtaggcg ggtatgccgg cgtggcggaa tgggggggca
     3421 accggtttgg gaatagcgtc atcacagccg tcgattctcc cctgacgatc acccagggtt
     3481 gcagctggcg gctgacgtcc ggaaagatac aactgtacaa tcccgtcgga ataaccagta
     3541 tgatctatgg tctggattct accggtgtgg caaccggatg tcctgacgga ggctctccat
     3601 actactacaa gctggcctgg acgggatcgg gacagaatcc ctataccatg gtccttccct
     3661 acccctgata atgacgccgt cggccgcggc ggggaatccg ctgaaaataa cttacattgc
     3721 cccggaagcc ggctatgaat ccccggctca tttatgcaaa ccgttacgac cgattggctc
     3781 cgctcggtgg atgtcttggc ggacgtaccg tccgatcagc tgcaatggct catcgatagc
     3841 agcaactgcc gcgaaatccc ggaagaagaa tatgttttcc ggtccggcga ccccgtaaag
     3901 ggcatgtaca tcgttctaaa tggcaagatc cggcttttca tcgttcagca aaacgagatg
     3961 cgggaggtag gcgtgctgga accccgggac gtcagcggag cgctgccctt ttccaggagt
     4021 gtcaaagcca cggtccatgc gacggccctg aaggatacgg cgatcatgat gctgcccgtc
     4081 gaaaaattcc gggagatggt ttcccggcac tacgagctga cggcagcgct ggtccacgtc
     4141 atgacggaca gggtcaggaa ttttacgacc cttcagcaac agaatgacaa gatgatggcg
     4201 ctgggtaaat tatcggcagg tctcgctcac gaattgaaca accccgcggc tgcaatagta
     4261 aggggttccg aatcattgaa acaacatctg cagctggaac ccgagtcgtt caaggacgtg
     4321 atcaatgtcc ggatggaagc aaaggatgtc gatttcttga aggataaact gttcgcgata
     4381 ttggcgcgca ccgataagcc ccggctgacc ctcgtgcagc ggacggagct ggaagatgag
     4441 atgcgcgatt ggctggacga gcagaaggtg cagaacagcg atgagatcgc cgagaacttc
     4501 ctcgactatg ccttcacgtg tgacgatatg gaacaattcc atgcgcatat ccctgatcaa
     4561 tatctctcgc cggtgctcaa ctggatcaat accaacctgg tcaccgagcg gatggtccag
     4621 gatatcaatg aggcgtccca acgcatcttc cgcctgatac agtcggtaaa gaatttcacc
     4681 catatggatc agggaaaagg aaaggagctc accgatatcc atacgggcat cgggagtacg
     4741 ctgacgatga tgcaatacaa actgaaaaag gccaatatcc gggtagtaga ggaattcgat
     4801 cccgccctgc cgaagataat ggcgcttgtc ggcgagctca accaggtatg gaccaatctc
     4861 atcgacaatg ccgtggatgc catggaggtc aatggcaagg gacagctgac catccgtacc
     4921 atgaaggacc acagtttcgc gaagatctcc gtcatcgatg acggcccggg tattccggcg
     4981 ggtatccggt ccaatatttt cgatcccttc ttcaccacca agggcatcgg taaaggtacg
     5041 gggctcggac tcgacgtcgt caaccggatc gtccagggcc agcaccgtgg ctctgtcaag
     5101 gtcgaatccg tacccggaag gaccgaattc atcgtctgcc tgcccatcgc gggctaaacc
     5161 aaacaaccat ggctttacct atcatattca gtattgacga tgacccgcag gtcctacgcg
     5221 ccatcacccg cgatctgcgg acacagttcc gacagtctta ccggatacta tccacgacat
     5281 cggtaaagga ggccctcgaa agcctgctcg atctgcgcaa taaaggagag accgtggccc
     5341 tgttcatctc cgaccagcga atgcccgaaa tggaaggcgt ggacttcctg caaaaagccc
     5401 gggaattcta tcccgatgcc aaaagagtcc tcctgaccgc ctattccgat acggatgccg
     5461 ccatcaaggc catcaatagc gtacagctcg attactatct catgaagccc tgggatccgc
     5521 cggaagaaaa gctgtatcct actatcaatg aactactcga tgactggcaa tccggctggc
     5581 gcccggcatt ccgggggatc aagctcatcg gttaccaatt ctcgccgaaa tctcatgaga
     5641 tcaaggaatt cctgtccgga aatctggttc cctatcaatg gcaggatatc gaatcgaacc
     5701 aggacgcgca acagtcgctg gaactgaata agatcgcctt aaaggacctc ccgacccttt
     5761 tcctcgaaga cggcagcttt ttctgtagtc cggagatccc gctggtagcc gagaagatcg
     5821 gtctgaac
//
LOCUS       DASYSV010000255         3492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_429677, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000255 DASYSV010000000
VERSION     DASYSV010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429677"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VGQ51_07835"
     CDS             <1..180
                     /locus_tag="VGQ51_07835"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036513.1"
                     /translation="HPNVLDRRDPEVIIYTVTSKTQEIKIYFWIKDVTKSAYTAGELK
                     TSIYRHFDEKGIAVI"
     gene            complement(213..1499)
                     /locus_tag="VGQ51_07840"
     CDS             complement(213..1499)
                     /locus_tag="VGQ51_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV9036514.1"
                     /translation="MKVTKRIFLTGILATTGMGLFAQNVDKANDLLKAGKITDAKAQI
                     DAALANPKNQKNATAWYYELKIYNAIAANDQLRAQYPGARDTAFLALKTYVDLDEKDK
                     KLLLLQVDGYKPINEIYQGYFQVGATDYNGGKYEDALQNFRGAIAASSYMTSKGWTKL
                     PIDTTSTLYAGISAEKAGKKDTAAIYYGKLADAKISAINGTNMIDIYKWLVDYYNTKK
                     DAANTEKYLTLAETEFPDDLFWPSTRLDNLREKGSKDSLWAEYERIIAHFPKNHLFYF
                     NYGLELYQYANDTSSGHRPDNYDALIAKAQENLKKCLELQPDYPQAALVLGQISYNAG
                     VDLQAQTKKITGKTPDDVKKRADLRIAAGKKFDEAIPYFEQVDKDLGSQGKLKMEQKS
                     ALKDAYDLLTNIYEDKRIKDKIDYWTNKYNNVDKDH"
     gene            complement(1535..>3492)
                     /locus_tag="VGQ51_07845"
     CDS             complement(1535..>3492)
                     /locus_tag="VGQ51_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEV9036515.1"
                     /translation="TGGIIYGMEGVKAAMHFGRGRVVLRAKLQVDTKASGREQIVITE
                     VPYQVNRDTLCDRIGQLVNEKIIEGIAHINNESNKREGTRIVIDLKRDAVANVVINQL
                     YKHSELQTSYGINNVALVKGRPRTLNIREMISEFVEFRMDVVTRRTRFELKEAEKKAH
                     ILQGYLIALDHLDEVIALIRASASPETAKESLINAGWGLDEIQAKAILELRLQRLTGM
                     EREKIRAEYDELLQLIARLQDLLADEGKRYEVIRKELEEVREKFGDKRKTEITYLDNE
                     VSIKDLIREEDVVITISHLGYIKRTSATEYRQQRRGGRGAMGGRTREEDFIEHLFVAS
                     THHTMLFFTVKGRCYWLNVYEIPDGEKNSKGRAVQNLIQLPPDDKVRAVVDVRDLKDE
                     AFVNTHHILLCTKQGVIKKTSLEEFSRPRQTGVNAITIVEGDMLLEARLTDGQCEVMM
                     AVKSGRAIRFEEEKVRPTGRGAIGVAGIEVDDRGDEVIGMICVNRQDKTRTVLVVSEK
                     GYGKRTHIDEYRITNRGGKGVKTISVTEKTGKLVGILDTLESEDLMITCKSGVTIRMP
                     VAGISEQGRATQGVKLIRLDENDEIAAITKLDKLEEEGNGHAAPPPEGNKENVESPGE
                     VTGENSEAPDGTTGETGDGPGETPGDL"
BASE COUNT          662 a         1021 c          867 g          942 t
ORIGIN      
        1 catcccaatg tgctcgaccg ccgtgacccc gaggtcatca tctacaccgt cacttcgaag
       61 acacaggaga tcaagatata tttctggatc aaggatgtca cgaaatccgc ctatacggcc
      121 ggagagctca agaccagcat ttaccggcat ttcgacgaaa aagggatagc agtcatataa
      181 acaaaagacc cggccgggcc gggtccttgt tattagtgat ctttatctac gttgttgtat
      241 ttgttcgtcc agtaatcgat cttgtccttt atccttttat cctcatagat attggtcagc
      301 agatcgtatg cgtccttcaa cgcgcttttt tgctccatct tcagcttgcc ctgtgagccc
      361 aggtctttgt ccacctgctc gaaatatggg atggcttcgt cgaacttctt tccggcggcg
      421 atacgcagat cggctctttt cttgacgtca tccggtgttt tacccgttat ctttttggtc
      481 tgagcctgca gatcgacccc cgcattgtag gatatctggc cgaggaccag ggccgcctgc
      541 ggataatccg gctgcagctc gagacatttc ttcaaattct cctgcgcttt ggcgatcagg
      601 gcgtcataat tgtcgggacg atgacccgaa ctggtatcgt tggcatactg gtaaagctct
      661 aacccatagt tgaaatagaa cagatggttc ttcgggaaat gcgcgatgat ccgctcgtat
      721 tcggcccaaa gcgaatcctt gctgcctttc tcgcgcagat tgtccaaccg ggtactcggc
      781 cagaacaggt cgtcgggaaa ttccgtctcg gcaagcgtca gatatttctc ggtattggcc
      841 gcgtccttct tcgtattgta gtaatcgacc agccatttgt agatgtcgat catgttggtc
      901 ccgttgatcg ccgatatctt ggcgtcggcc agctttccgt aatagatcgc ggcggtatcc
      961 ttcttacccg ccttttcggc ggagatgccg gcatacagcg tggaagtcgt gtcgatggga
     1021 agtttggtcc atcctttcga cgtcatatag ctgctggcgg cgatggcgcc cctgaaattc
     1081 tgcagggcgt cctcatattt ccccccattg taatcggtgg cacccacctg gaaataaccc
     1141 tggtagatct cgttgatggg cttatagccg tccacctgca gtaacaacag cttcttatct
     1201 ttctcatcca ggtcgacata agtcttgagc gccaaaaatg cagtatcccg cgcgccggga
     1261 tattgggcac gcagttgatc gttggccgca atggcgttgt agatcttcaa ctcgtaatac
     1321 caggcagtgg cattcttctg atttttcgga ttggccagag ccgcgtcgat ttgcgctttc
     1381 gcgtcggtga tcttaccggc cttcaacaga tcgttggcct tatcgacgtt ctgcgcgaaa
     1441 aggcccatac cagtagtggc aaggattcca gtcaggaata tccgtttagt cactttcatt
     1501 tttagaatgt ttattaattg aaaacgattt agtctcaaag atccccgggg gtctcacccg
     1561 gaccgtcacc ggtctcaccc gtagtcccat ccggggcttc actgttctca cccgtaacct
     1621 caccggggct ctctacattc tccttattcc cttcaggagg cggcgctgca tgtccgttcc
     1681 cctcttcctc cagcttatcc agtttggtaa tagcggcgat ctcgtcgttc tcatccaggc
     1741 ggatgagctt gacaccctgg gtggccctgc cctgttcact gataccggcc accggcatac
     1801 gaatcgtcac accgctctta caagtgatca taaggtcttc agattcgagg gtatccagaa
     1861 tccccactaa tttaccggtt ttctctgtaa cgctgatagt ttttacccct ttgcctcccc
     1921 ggttggtgat acggtactca tcgatatggg tccgcttgcc atagcccttc tcactgacca
     1981 ccaatacagt ccttgtctta tcctgtctat tgacacagat catgccaatg acctcgtctc
     2041 ccctgtcgtc cacctcgatg ccggctacgc caatcgcgcc cctgccggtc ggacggacct
     2101 tttcttcctc aaaccggatg gcgcggccgc ttttcacggc catcataacc tcgcattgac
     2161 catccgtcaa cctggcttcc agcagcatgt ctccttctac gatggtgatg gcgttgaccc
     2221 cggtctgccg cgggcggctg aattcctcca gcgaggtctt tttgatgacg ccctgtttgg
     2281 tacagagcag gatgtgatgc gtgttgacga acgcttcatc tttgagatcc ctaacgtcga
     2341 ccaccgccct gaccttatcg tcgggaggca gctggatgag gttttggacc gcccggccct
     2401 tgctgttctt ttcgccatcg gggatctcat agacattgag ccagtaacaa cggcccttta
     2461 cggtgaagaa aagcatggtg tggtgtgtgg aggccacgaa cagatgttcg ataaagtctt
     2521 cctcccgggt cctgccaccc atcgccccgc gaccgccccg acgctgctgc cggtattccg
     2581 tcgcggaggt ccgtttgata taacccaaat gggagatcgt aatgaccacg tcctcttccc
     2641 ggatgaggtc cttgatgctg acctcgttgt ccaggtaggt gatctcggtc ttccgcttgt
     2701 ccccgaattt ttccctgact tcctccagct ctttgcggat cacctcataa cgcttgcctt
     2761 cgtcggccag cagatcctgc agccgggcga tcagttgcaa gagctcgtcg tattcggcgc
     2821 ggatcttttc tctttccatg cccgtaagcc gctgcagcct cagttcgagg atggccttgg
     2881 cctggatctc atcgagtccc cagccggcgt tgatcaggct ctcctttgcg gtctcgggtg
     2941 aagccgatgc acggataagg gcgatcacct cgtcgagatg atccagcgcg atcagatagc
     3001 cttgcaggat atgcgctttt ttctcggctt ctttcagctc gaaccgcgtc ctgcgggtta
     3061 ccacgtccat ccgaaactcc acgaactccg agatcatctc ccggatgttg agggtcctcg
     3121 gccggccctt gaccagcgcg acgttgttga taccgtaaga cgtctgaagc tcgctgtgtt
     3181 tatacagctg gttgatcacg acgttggcta ccgcgtcacg cttgagatcg atgacgatac
     3241 gtgtgccctc gcgtttgttc gattcgttat tgatatgggc gatgccctcg atgatctttt
     3301 cgttgaccag ttgtccgatc cggtcgcaaa gcgtgtcacg gttcacctgg taaggcactt
     3361 cggtaatgac gatctgttcc ctgccactgg ccttggtgtc cacctgcagc ttggccctca
     3421 atacgacgcg gcccctgccg aaatgcatcg ccgccttaac gccttccata ccgtagatga
     3481 ttcccccggt ag
//
LOCUS       DASYSV010000256         8111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_819395, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000256 DASYSV010000000
VERSION     DASYSV010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819395"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VGQ51_07850"
     CDS             <1..537
                     /locus_tag="VGQ51_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV9036516.1"
                     /translation="DAAIAGQQFVISPWLDESLRKSYDDVLRFMEVFNKCGELCKAQG
                     MKFGYHNHDFEFKTVINNQKLYDIILQHTDPELVAQQLDMGNLYDGGAVAIAIVMQYP
                     GRFELMHVKDEIKATSGTEPFESTILGQGIVGTKQVCDQGRKTGTRCFIIEQESYQGK
                     APLDCSRADLEIMKKWGY"
     gene            complement(592..2835)
                     /locus_tag="VGQ51_07855"
     CDS             complement(592..2835)
                     /locus_tag="VGQ51_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV9036517.1"
                     /translation="MTKSSLISAVLLFSLTVATAQTAADRQKADNLLRQMTLDEKIGQ
                     MTQVTLGVVSTAEDGVLDPAALKKAVQDYKVGSILNVTNHALTVDQWRHVQTAIQDEA
                     MHTRLKIPVIYGLDGIHGQTYTLDATLFPQSIAMAATRDPELVFAAAKVAALELRASG
                     VRWNFAPVLDCGRQPLWSRFPETYGEDVYIGKTMGVAAIKAYEGDGLRNPTAVASSMK
                     HYVAYSDSRTGKDRTPIYLPEIEMREYYLPQFRDAIKAGSSTLMVNSSEINGTPVHAS
                     HYLLTDVLRKELGFQGVVVSDWEDIIRLHTRHNVAATPRAAVVLAVNAGVDMSMVPSD
                     FSFFILLKEAVQKGEVPMSRIDEAVRRILLLKLKVGLFDNPYPESAANFGKPQYQTLA
                     LTAAREAMTLLRNEGDVLPLNRGTRILVAGPAARSISALNGCWSYTWQGKDERWYPKD
                     SKTIVDALTDRLGADHVATTSVPVFDSPANYDTTALKNAAAGVDAIVLCLGENAYAES
                     PGNIRDLALPDQQLALARAAAATGKPVILVLTEGRPRLITGIVPMMKGILLAYWSGRK
                     TAEAITDVLTGEYNPDGRLPYSYPRSMGEMVLYDRKPTEDIREIFNSDMTMNGYDPLF
                     PFGWGLSYTTFTYGPITLSSPGLSGSGKLTVSLTVTNSGKKDGKNTVELYTREHYASV
                     TPNMRRLRAFEKIFLKAGESKTVSFTLDRNDLAFVNAQLKTVTEPGDFDIYIGDQRKT
                     FAYTTAP"
     gene            complement(2904..3380)
                     /locus_tag="VGQ51_07860"
     CDS             complement(2904..3380)
                     /locus_tag="VGQ51_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036518.1"
                     /translation="MKAASIHEIKQELLSLSPSRLTEICLRLARFKKDNKELLTWLLF
                     EATDETAFIFSVKKEIDLEFDGLPKPNLYLTKKSLRKVLRSTAKQIRYTGSPQAEVEL
                     LSHFLRKLDNSGIPYRDSPVLMNLYRQQLKKIRSVIAGLHADLQYDYLRELEGLEG"
     gene            complement(3390..3896)
                     /locus_tag="VGQ51_07865"
     CDS             complement(3390..3896)
                     /locus_tag="VGQ51_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV9036519.1"
                     /translation="MKKFIAWLLDPPVFGAPAIIWIRLLTGGIFFWEGLLKFAYPNQG
                     VGRFTKLGFPFPEFTAHFVAVTEIMGGLFLLAGLFTRVAALYFIAEMIVAILSTKIGV
                     YLGTSPLPLPPSPPKTGFWAVLHEIRADYAQLLTGIFLLMEGPGPRSVDRIMRRGLPV
                     TIDPSKIE"
     gene            4065..5111
                     /locus_tag="VGQ51_07870"
     CDS             4065..5111
                     /locus_tag="VGQ51_07870"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9036520.1"
                     /translation="MRQRRVWIAIGLWSGLYVLWILLFHDHVFTVTRTLTVQFCYLLF
                     VFANYYIQVYYAIPRLLHRKRYLLFGMLLPLGIAVSALIRTPLAIWLQTHVFLPGHPA
                     PDADGVFLDSFLNISIWVVFAIAVRQVIEKINFRQHIDSLERQRIRNELDFLKAQFNP
                     HFLFNSINSIYGHIDKRNGKARDMLLSFSEMLRYQLYECNVDKIAIERELQYIRHYVA
                     LQQERKGGSLRVRLDIPENIGGFTIAPLLLITFVENAFKYVGSDEDSDNEVRLSFRKH
                     RDELLFRAFNTKEATSPADGVKGGIGIVNARRRLDLQYPDRHRLTITDGDGSYEVVLN
                     LNIKAHVTESHYSG"
     gene            5083..5820
                     /locus_tag="VGQ51_07875"
     CDS             5083..5820
                     /locus_tag="VGQ51_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9036521.1"
                     /translation="MLLKAIIVDDEPIARKVLSEYIEDIDYLELAGMAENPLKADALI
                     AVRPVDLIFLDINMPKLSGIEFLRARAAGEKRPLVIITTAYAEYALDGFELDVVDYLL
                     KPISFERCVKACHKARLKYAERLPGPATAATAAHEDHFFVKCDDGLEKVAYNELLFVE
                     AMMNYVVLHTAGRKLIVYLTLKSILSQLPAQRFIQVHKSFVVNKDSITGIRGNTIRMG
                     AISIPISQHFHETAMREIVKDRIIKRN"
     gene            5916..7103
                     /locus_tag="VGQ51_07880"
     CDS             5916..7103
                     /locus_tag="VGQ51_07880"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV9036522.1"
                     /translation="MNKDVVIVSAVRTPIGSFGGALKDFSAVQLGAVAIRGALERAGL
                     KPEQVQEVLMGCVLQGNLGQAPARQAAKGAGLPDEVVCTTVNKVCASGMKAVMQGAQA
                     IALGDSGIVVAGGMESMSNVPFYLEQLRWGNKYGNTTLIDGLAKDGLTDAYDGKAMGN
                     AAELCASSCGISREEQDAFAIASYRRSQAAWAGGRFDEEVVPVEIPQRKGGTVFFGKD
                     EEPFNVKFEKIPELKPSFLKDGTVTAANASTLNDGAAAFVLMSSGRAAELGLKPLARI
                     AGYADAEQAPEWFTTTPAIALPRAVAKAGIRMEDVSFWELNEAFSVVGIENTRRLKID
                     PEKVNVNGGAVSMGHPLGASGARILVTLLYVLRQNKARYGAAGICNGGGGASALVLEN
                     LAL"
     gene            complement(7168..7521)
                     /locus_tag="VGQ51_07885"
     CDS             complement(7168..7521)
                     /locus_tag="VGQ51_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV9036523.1"
                     /translation="MHYLGKNIRYLRKQLSKTQSEIASLIKKGQTTIGNWENGISEPS
                     LEELLILSNFFDIPLDTLIRIDLAETNIPHRNTAGIYYDHTPEPSPLVREDQLTYVLQ
                     EIKSLREEMERRLPK"
     gene            7642..>8111
                     /locus_tag="VGQ51_07890"
     CDS             7642..>8111
                     /locus_tag="VGQ51_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036524.1"
                     /translation="MNTSLKHLSPGQREKIERAVRIIVRAVQPEKIILFGIYGGNGGL
                     AAAKISRWPKGLGAYDLLVITRQGDGRSDYELQDLIENRCREEVAVTALVHDIGYLNR
                     RLTEGQCFFTMAVLEGILVYDASRTPLAEICLPDWERVRWMAQRDFQRWNEQACA"
BASE COUNT         1783 a         2014 c         2483 g         1831 t
ORIGIN      
        1 gacgccgcga ttgcgggtca gcaattcgtg atcagtccct ggctggacga gagtctgcgg
       61 aagtcctatg acgacgtcct ccggttcatg gaggtgttca ataaatgcgg cgagctgtgc
      121 aaggcgcagg gtatgaagtt cggctatcac aaccacgatt tcgagttcaa aacggtcatc
      181 aataaccaga agctgtacga tatcatcctg cagcataccg atccggagct ggtcgcccaa
      241 cagctggata tgggcaatct ttacgatgga ggcgcggtcg cgatcgcgat cgtgatgcag
      301 tatccggggc ggttcgagct gatgcacgtc aaggacgaga tcaaggctac cagcggcaca
      361 gagccttttg agagtacgat cctgggacag ggcatcgtcg ggaccaaaca ggtctgcgat
      421 cagggcagga agaccggtac cagatgtttc atcatcgaac aggaatccta ccagggcaag
      481 gctccgctgg attgctccag ggccgatctg gagatcatga agaagtgggg ctattagtcc
      541 gagaccttca cgcgcccggg cgcggggcta ctgagttggc gacgcgcggg gtcagggcgc
      601 cgttgtatac gcaaacgtct ttcgctggtc cccgatatag atgtcgaaat cccccggctc
      661 ggtcaccgtc ttcagctgtg cattgacgaa agccaggtcg ttcctgtcca gggtgaagct
      721 cactgttttc gattcccccg ccttcagaaa tatcttttca aaagcgcgca accgccgcat
      781 attgggcgtg acgctcgcat agtgttcacg cgtatagagc tctacggtat tcttgccgtc
      841 ctttttgcca ctgttggtta ccgttagact gaccgtaagc ttgcccgaac cgctgaggcc
      901 cggcgaactg agcgtgatgg gaccataggt gaaggtggta tagctgagac cccagccaaa
      961 ggggaacagg gggtcgtatc cattcatcgt catatcggaa ttgaatatct cgcggatgtc
     1021 ttcggtgggc ttccggtcgt agagcaccat ttctcccatg ctccgcgggt agctgtaagg
     1081 caggcgtccg tcggggttgt attcacccgt cagcacgtcg gtaatggctt cggcggtctt
     1141 tcggccgctc caataggcca gcaggatacc tttcatcatc ggaacgatgc ctgtgatcaa
     1201 ccgcggtctg ccctcggtga gcacgaggat gacaggttta ccggtagcgg cggcggcgcg
     1261 tgcgagggcc aattgctggt ccggcagggc aaggtcgcgg atattgccgg ggctttccgc
     1321 ataggcgttc tcgccgagac agaggacgat ggcgtccacc ccggccgcgg catttttgag
     1381 tgccgttgtg tcgtaattcg cagggctgtc gaaaacagga acggacgtgg tggcaacatg
     1441 gtctgcgccc aaacgatcgg tcaacgcatc cacgatcgtc ttgctgtctt tggggtacca
     1501 gcgttcgtcc ttaccctgcc aggtatagct ccagcagccg ttgagcgcgc tgatgctacg
     1561 ggccgcaggg cctgctacca ggatacgggt cccgcggttc aatggcagga cgtctccttc
     1621 attcctgagg agggtcatgg cttcccgcgc ggcggtcagc gccagggtct ggtattgagg
     1681 ttttccgaaa ttggcggctg attcgggata ggggttgtcg aatagcccta ctttcagttt
     1741 gagcaggagg atgcgccgga cagcctcgtc gattcggctc atcggtactt cgcctttctg
     1801 gaccgcctct ttgaggagga tgaaaaagga aaaatcgctg ggcaccatgc tcatatcgac
     1861 accggcgttg acggccagca cgaccgccgc ccgcggcgtg gccgcgacat tatgacgggt
     1921 gtggaggcgg atgatgtctt cccagtccga gaccaccaca ccctgaaaac cgagctcctt
     1981 gcgaaggaca tcggtcagca gatagtgact tgcatgaacg ggagtcccgt tgatctcgga
     2041 ggaattgacc atcagggtgg acgatcccgc ttttatggcg tccctgaact ggggcaggta
     2101 gtattcccgc atttcgatct ccgggagata gattggggta cggtccttcc cggtgcggct
     2161 gtccgaataa gccacgtagt gtttcatgct gctggcgacg gcggtcgggt tacgcaggcc
     2221 atcgccttca taggctttta tggcggcaac acccatggtc ttgccgatat agacgtcttc
     2281 gccgtaggtc tccgggaaac gcgaccacag cggctgacgt ccgcagtcga gcaccggcgc
     2341 gaaattccag cgtactcccg aggcccgcag ctcgagcgcg gccaccttcg ctgccgcgaa
     2401 gaccagctcg ggatcacgtg tggcggccat ggctatgctt tgggggaaca gggtagcatc
     2461 cagcgtatag gtctgcccat gtatgccatc caacccatag ataacgggga tcttcaaccg
     2521 cgtatgcatc gcctcatcct gtatcgccgt ctgaacgtga cgccattggt cgacggtcag
     2581 cgcatggttg gtgacgttga ggatcgagcc gactttataa tcctggacgg ccttcttcag
     2641 cgccgcagga tcgagtaccc cgtcttccgc cgtcgatacg acacccagcg tcacctgggt
     2701 catctgcccg atcttttcat ccaatgtcat ctgccggaga aggttatcgg ctttttgcct
     2761 gtctgctgcg gtctgcgcgg tggcaaccgt cagcgagaac aggaggacgg cggaaataag
     2821 cgaactttta gtcatatagc cataaagata aggagacgga tcacctgccg gggcatgaac
     2881 ttttgatcaa ttccgataag attttatccc tccaacccct ccagttccct gagatagtca
     2941 tactgaaggt ctgcatgaag tcccgcgatg acggaacgta tctttttgag ctgctgacgg
     3001 taaagattca tcaggacagg actatcccgg tacgggatgc ccgaattgtc cagcttgcgg
     3061 aggaaatggc tcagcagctc aacctccgcc tggggggagc cggtataacg gatctgtttg
     3121 gccgtagacc ggagtacctt acgcaggctt ttcttcgtca ggtagagatt gggtttcgga
     3181 aggccatcga attcgagatc gatctctttc tttaccgaaa agatgaaggc cgtctcatcg
     3241 gtggcctcga aaagcagcca ggtcagcagt tccttgttgt cctttttaaa acgggccagg
     3301 cgcagacata tctctgtaag acgggatggg gacagcgaga gcaattcctg tttgatctcg
     3361 tggatggagg cggctttcat ccgcgcaaat tattcaattt ttgacggatc gatggttacc
     3421 gggagcccac ggcgcatgat cctgtcgaca gaccgggggc ccggaccttc catcagcagg
     3481 aaaatgccgg tcagcagctg tgcatagtcc gcgcgtatct catgtagcac ggcccagaaa
     3541 ccggttttgg gaggggaggg cggcagcggg aggggcgagg tgccgagata gacgcctatc
     3601 ttggtcgaca atatggcaac gatcatttcg gcgatgaaat agagtgctgc gacacgtgtg
     3661 aatagtccgg ccagtaagaa cagtcctccc attatttcgg tgacggcgac gaaatgcgcc
     3721 gtaaactccg ggaaagggaa tcccaatttg gtgaatcggc cgacaccctg attggggtag
     3781 gcgaatttca aaagtccttc ccagaaaaat atgccgccgg tgagcaggcg gatccagatg
     3841 atggcgggag cgccgaaaac gggcgggtcg aggagccagg cgatgaattt tttcatttgc
     3901 tgttattttg ggcaaaacaa cggagagacg cgggccgccg caaatttttt acatcacccg
     3961 gcggcggtcc ttgataaggc cggctttccg catgtcatcc cggttttcgg gcacttcatg
     4021 taaaatcgcc tgtctgcgcc gtgctgaatg gtaactttag gggaatgaga cagcgacgcg
     4081 tctggatagc gatcggcctt tggtccggcc tgtatgtgct ctggatcctc ttgttccatg
     4141 accatgtctt cacggtcacg cggacgctga cggtgcagtt ctgctatctg ctgttcgtct
     4201 ttgccaatta ttatatccag gtgtattatg ccatcccgcg attgcttcat cggaagcggt
     4261 atctgctttt cgggatgtta ttaccactcg ggatcgcggt gagcgcgctg atccggacgc
     4321 cgctggccat ctggctgcag acgcatgtct ttctgcccgg tcatccggcc ccggatgcgg
     4381 acggggtctt tctcgattcg ttcctgaata tttccatctg ggtggttttt gcgattgcgg
     4441 tccggcaggt gatcgaaaaa ataaatttcc ggcagcatat cgattctttg gagcggcaaa
     4501 ggatccgtaa tgaactggat ttcctgaaag cgcagttcaa cccgcatttc ctgttcaact
     4561 ccatcaactc gatatacgga catatcgaca agcgaaatgg aaaagccagg gacatgctgc
     4621 tgagcttttc ggagatgctg aggtatcaat tatatgagtg caatgtggac aagatcgcca
     4681 tagaaaggga gctgcaatat atccgtcatt atgtggcgct gcagcaggag cggaagggcg
     4741 ggagcctgcg ggttcggctg gacattcccg aaaatatcgg cgggttcacc atcgctccat
     4801 tattgctgat caccttcgtc gaaaacgcct ttaagtatgt cggctccgac gaagacagcg
     4861 ataatgaggt ccggctgtcc ttccggaagc atcgggacga gctgctcttc cgggccttca
     4921 atacgaagga ggcgacctct ccggcggacg gcgtgaaagg aggtatcggc atcgtcaatg
     4981 cgagacgcag actggacctt caatacccgg atagacatcg cctgacgatc actgacggag
     5041 acggttcata tgaggtcgtt cttaacctga atatcaaagc ccatgttact gaaagccatt
     5101 atagtggatg atgagccgat agcgcggaaa gtcctttcgg agtatatcga ggacatcgat
     5161 tatctcgagc tggcgggtat ggccgaaaat ccgctgaagg cggatgccct gatagcggtg
     5221 cgtcccgtgg acctgatctt tctcgacatc aatatgccga agctgagcgg tatcgaattc
     5281 ctgcgggccc gggcggcggg agaaaagcgt ccgctggtga tcattaccac cgcatatgcc
     5341 gaatatgcgc tggacggttt cgagctggat gtggtggatt atcttttgaa acctatatcc
     5401 ttcgagcggt gcgtaaaagc gtgtcataag gcgcggctga agtacgcgga gcggctcccc
     5461 gggccggcaa ctgctgcaac cgctgcgcac gaggaccatt tcttcgtcaa gtgcgatgac
     5521 ggtctggaga aagtggcgta taatgagctg ctctttgtgg aggcgatgat gaactatgtc
     5581 gtgttacata ccgcgggtcg taagctgatc gtctatctca ccctgaaaag catcctttcg
     5641 cagctgcctg cgcaacgatt catccaggtg cacaaatcgt tcgtggtgaa taaggattcg
     5701 atcaccggta tccggggtaa taccatccgg atgggggcca tcagcattcc cattagccag
     5761 catttccatg agacggcgat gcgcgagatc gtaaaggaca ggatcatcaa aagaaattga
     5821 ccgggcgcta acaagtgtta gctttggatt atatttgcgg acggcgccga tacaacggct
     5881 cgtttggcgg gattgcccgg taaaatgaat cgcatatgaa taaagatgtt gtcatcgttt
     5941 cggcagtaag gacgccgatc ggcagtttcg gcggcgcttt aaaggatttt tccgcagttc
     6001 agcttggcgc cgtcgccatc cgcggggcat tggaaagggc gggcctgaag ccggagcagg
     6061 tacaggaagt gctgatgggt tgcgtcctgc agggtaacct ggggcaggcg cccgcacggc
     6121 aggcggcgaa gggcgcggga ttgccggatg aagtggtctg tacgaccgtg aacaaggtct
     6181 gcgcgagcgg gatgaaagcg gtgatgcaag gcgcacaggc gatcgcgttg ggggattcag
     6241 gtatcgtcgt agcgggcgga atggaaagca tgagcaacgt accgttctat ctggagcaat
     6301 tgcggtgggg gaacaaatat ggcaatacta cgctgatcga cggtctggcg aaagacggat
     6361 tgacggatgc gtatgatggg aaggcaatgg ggaatgccgc ggaattatgc gcgtccagtt
     6421 gcgggatcag ccgtgaagag caggatgcgt ttgcgatagc aagctaccgg cgaagccagg
     6481 cggcatgggc cggcggcagg ttcgacgaag aagtggtgcc ggtggagata ccccagcgaa
     6541 aaggaggaac cgtatttttc ggaaaggacg aagagccatt caatgtaaaa ttcgaaaaga
     6601 tacctgagct gaaaccgtcg ttcctgaagg acggtaccgt tacggccgcc aatgcttcca
     6661 ctctgaatga cggagccgcg gcgtttgtat tgatgagcag cgggcgggcg gcggagctgg
     6721 gattgaaacc gctggcgcgg atcgcgggct atgccgatgc ggaacaggcg ccggaatggt
     6781 tcactacgac accggctatc gcattaccca gggcagtggc taaggccggt ataaggatgg
     6841 aagatgtttc cttttgggaa ttgaatgagg ccttttcagt ggtagggatc gagaatacga
     6901 ggcggttgaa gatcgatccg gaaaaggtga atgtcaacgg aggcgcggta tcgatgggtc
     6961 atccgctggg cgccagcggc gccaggatac tggtcacgct gctgtatgtg ctgcggcaga
     7021 ataaggcccg ttatggggca gctggtattt gtaacggggg cgggggtgct tctgcgcttg
     7081 tgctggagaa tcttgcgtta tagacccatg aagccggggg gggcgacgca ttcccagcag
     7141 gcttcgaaga agccgggggg cgacgcatta tttcggaaga cgtcgttcca tttcttctct
     7201 caatgatttg atctcctgta agacatacgt cagctgatct tcccttacaa gaggggaagg
     7261 ctcgggcgta tggtcataat aaatgccggc ggtgttccga tggggaatat tcgtttccgc
     7321 cagatcgatc ctgatcaatg tgtccagcgg aatatcaaaa aaattgctaa gaatcaacag
     7381 ttcctcgaga ctgggttcgc tgatgccgtt ttcccaattc ccgatcgtcg tttgtccctt
     7441 tttgatcagg gaagcaatct ccgactgggt tttagagagt tgcttgcgta gataacgtat
     7501 gttctttcct aagtagtgca tagcaagaag atcgggtttt ctgaaaatgt cagtaaaaaa
     7561 attggtaaat ctgaggaaaa aaattgatat tcgtaattcg caaatttagc caaattaatt
     7621 ggtagtcgaa cccatcataa tatgaatact tcgttaaaac atctgtcgcc gggtcagcgc
     7681 gaaaaaatag agagagccgt ccggatcatc gtccgggccg ttcagccgga aaagatcatt
     7741 cttttcggga tctatggggg gaatggaggg ttggcggccg cgaagataag cagatggccg
     7801 aaaggactgg gcgcgtatga tctgctggtg atcacgcggc agggtgatgg tcgctccgat
     7861 tatgagttgc aggatctgat cgagaaccga tgtcgcgagg aggtggcggt taccgcgctg
     7921 gtccatgata tcggctatct gaacaggcgt cttaccgagg gacagtgttt cttcacgatg
     7981 gctgtgctgg aagggatatt ggtatatgac gcgagccgga cgcctcttgc cgaaatatgt
     8041 ctccctgact gggagcgcgt gcgatggatg gcgcaacgcg atttccagcg atggaatgag
     8101 caggcttgtg c
//
LOCUS       DASYSV010000257        10537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1069660, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000257 DASYSV010000000
VERSION     DASYSV010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10537
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069660"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VGQ51_07895"
     CDS             complement(<1..613)
                     /locus_tag="VGQ51_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9036525.1"
                     /translation="MKSAPLLSIVLFLCISFSSYAQEYSYTHYDIADGLAGSVVYCIT
                     QDKDGFLWMGTETGVSRFDGTHFKTFTVADGLPDIEVLKMFTDSKGRVWMAPFRKSVC
                     YYYRGMIHNQDNDSMLGRIRLRENVEDFAEDANGDILILEQTRLHWLEAGGKVREIDS
                     IGSSPMRNAMTACRSASGHFLVQTDRNIYEFSGRSSRLIYSFPM"
     gene            complement(620..3559)
                     /locus_tag="VGQ51_07900"
     CDS             complement(620..3559)
                     /locus_tag="VGQ51_07900"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9036526.1"
                     /translation="MKCARLLSCVLFINLPFSSFAQEYSYTHYDITDGLAGSVVYCIT
                     QDKDGFIWAGTETGVSRFDGTHFRTFTAADGLPDIEILEMFGDSKGRVWMAPFRKSVC
                     YYYQGKIHNQQNDPMLSRIHLTENVEDFAEDSAGNILIMEKPALHWIGADGTVREIDS
                     LGGRPIESAIAISRSDSGNFLVATAGKVFTMSDKGSALFRQFYIKTPMSTYISLNPKG
                     MIWHTDLNSYDISAFASGKITRLNWNRKYYMHVSFSSVGDSLFFKNERTGTIGYNLNT
                     GKYKRFLPGTEVSKTFRDATGNIWFTTMGHGIYRLNSDEFRTIRVGLPGMQEAGVYAF
                     RRIGDELVVGDDFNTLYTFSISTEGLVRRKVPYGLFGARSRILYLDKMSSGGLFLATD
                     GGINRTPAHSRYGEPVLMGVKAVFKTKADQYLVAAGWGACLFDARKWKITDTLWRERT
                     TTIFSRNDTCYIGTLNGLYRLNPDRSSVYLGSTDPFFQRRISGIVESSDGTLWIASYD
                     GGVIAYRDNRVIALIDKSNGLNSNICRTMFIAENTLWVGTDKGLNKIALDRAGYPVLH
                     YTSKDGLGSDIINTIYVDGPVVYVGTPAGLSYFDQTKVNGMSDCRLHLLSIANSGKDR
                     IGDSADLLIPYQDRHVRFEFAAISYRSVGDIKYRYRMLGLDTTWQETKEGFLEYPFLP
                     SGHFVFQLQAINKFGIESRLLKMSVEVATPFWQAAWFYVLILVILVLLVGLLFNMRIK
                     RIHRRQEEKEQVSHRLAELENTALQAQMNPHFIFNCLNSIQQYIFDHEALAANRYLTG
                     FARLIRATLHNSSRNFISLADEVDYLSTYLSLEKLRFKEKMDYKVEIDPSIDMELFVI
                     PPMLIQPFVENSMRHGLRHKTDGTGSILILFKRSDEKLMVIVEDNGIGRKKAAAYKTG
                     EHIEYQSKGMSLTADRIRMMNVKYRDSIHVEVRDMEDGNGRAAGTRVTLEFPVFHSPT
                     QKDSLFL"
     gene            complement(3556..4554)
                     /locus_tag="VGQ51_07905"
     CDS             complement(3556..4554)
                     /locus_tag="VGQ51_07905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9036527.1"
                     /translation="MALSIVLINFKSPQLHIDCLTEIYRDPVGYTFEVIEVDNNSGDD
                     SRERILGAFPQVKWVQLDSNAGFARGNNAGMRVAGGDAMLILNGDTLPRGKDIEECYR
                     RLLNSGYVAAGVQLLNPDGSHQISGNYAMKGGVNYLLMMPYLGAFFKWLGYTFKVKKP
                     HVPDQQKEIEVDWINGAFLMVKKEAIDKAGMMDEDFFLYAEESEWCGRLRKLGKMAIY
                     GDLEVIHLQGVTANLAFGSDGKGYVNIFDRKGLQLLLSNFVRIRKEFGVGWFLFQLLC
                     YIVNIPIFFLGMLWAGLTGKKRYTWTKFRQYCRNVGIVIRKSPTIIRNKPYFYKVI"
     gene            complement(4554..5504)
                     /locus_tag="VGQ51_07910"
     CDS             complement(4554..5504)
                     /locus_tag="VGQ51_07910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9036528.1"
                     /translation="MEECVCTLEGIMNSPRLTIIIPTLQAGRTLHDSLDSVLCQRFRD
                     FEILIMDSVSRDNTLDIARGYAAKDHRVRVQSSPDKGVYDAMNKGVAEARGEWVFFLG
                     SDDRLHNEHVLETFFSSPGVSDFDMVYGNVVSPAFKGVYDGPFTLEKLMHRNIPHQAV
                     FYKKDVFRIVGGFRIRYKAYADWDLNIRCFNEPRVRNRYIDLIVADFGAEGVSSRHDV
                     PFLREVMISEKLRWLNRAGIRTLRSVKVFDEWWRLLRNAAVRDGDDLKECVNGEKIPQ
                     PLRRMIDWQSRIPPRFLRKGFFSKPVMFLNYISNLLTASF"
     gene            complement(5468..6370)
                     /locus_tag="VGQ51_07915"
     CDS             complement(5468..6370)
                     /locus_tag="VGQ51_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036529.1"
                     /translation="MPVVLTNLSNQLFEESRMRLNRSASKYGIDDIRSYDFEQIRQTP
                     FFAANKEILDHPTGMGYWLWKPYIIREALNTVQDGDIVIYSDSGIEIIASPDPLLSLC
                     REQTPVLLFGNADNTNAAWTKRDCFILMGCDSNYFWKGPHCDAAFGLFRKCPLTMQFV
                     GEWLEYCRDPRILTDSPNTSGKRDLPDFIEHRRDQSVLSLLALKYRLPLFRMPTQFGN
                     HYKMPAFRVDNEFNCVDQYRQHAVDYYAAIPYYNSGYFQLLDHHRGRSEGQPGRKNTT
                     PFIWRILKKRLTRWRNAFALWRES"
     gene            complement(6372..7193)
                     /locus_tag="VGQ51_07920"
     CDS             complement(6372..7193)
                     /locus_tag="VGQ51_07920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV9036530.1"
                     /translation="MLDAFMQLFQKKIPYKSEIPFSPAELSGYMRANRKVLGEVDDWI
                     DEQAFARSFFNYGVPDSIKEVINKPIGDAPTYTDLMTLIADRYLGSVNYLEIGISVGK
                     NFFQLLKTLPQASLTAFDIEEINPVLASRLVAKSKTEWATPSGSVKKNQSSLSEFSFG
                     GKKVSYVSGDVWDANSWARLEGNSFNLVFSDALHSPEAILFEFEMLVKYRLLGERFVI
                     VWDDLVGKMKNSFFRIIRRYNETYGIRDIYLLEVNGWVGQHEGAHSVGIISNFEF"
     gene            complement(7224..7988)
                     /locus_tag="VGQ51_07925"
     CDS             complement(7224..7988)
                     /locus_tag="VGQ51_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEV9036531.1"
                     /translation="MDRKIRVGAVSYLNTKPLLYGIERASIRKQIELIIDHPASIASM
                     LVRDEIDMGLVPVAVIPFLPEYHINGNHCIGSNGDVASVCIFSETPMERIERLLLDYQ
                     SRTSVQLAKILLREYWKIQPELVSTSSDFRNQIRGTTAGVVIGDRALEQRLRSPYIYD
                     LAGAWKDLTGLPFVFAAWISNKKLDSSFLAEFDEANRQGLAHIDAVVAENPYPAFPLN
                     DYFTKHLSYTLDPLKRGGLERFLQYLQVSAAPLVQK"
     gene            8017..9363
                     /gene="purB"
                     /locus_tag="VGQ51_07930"
     CDS             8017..9363
                     /gene="purB"
                     /locus_tag="VGQ51_07930"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV9036532.1"
                     /translation="MELNALTAISPLDGRYRHQVQHLDDYFSEYALIRYRVIVEVEYF
                     LFLGRKKFFTLDAPGRRALQAIAAQFSLEDAQWIKETEKTTNHDVKAVEYFLKDRLTK
                     ATPTDAALGEWIHFALTSQDINNTAIPLLWKHAVEFEYLPGLLNLKRQLEVLARDWKD
                     IPMLARTHGQPASPTLLGKEILVFIERLEDQVELLIQVPFSAKFGGATGNFNAHNVAF
                     PGKDWVKLANEFVEGTLGLQRQQYTTQIEHYDNLAAHFDAIGRINTILIDLCRDLWTY
                     VSMDYFTQKTKKGEIGSSAMPHKVNPIDFENAEGNLGLANALFGHFSAKLPISRLQRD
                     LTDSTVLRNIGVPFAHTILALNSIGKGLDKLVLNRARLQQDLENNWAVVAEAIQTVLR
                     RENYPKPYEALKELTRGKSGITKQSLHKFIDGLKVSAAVKKELKKVTPHNYTGISY"
     gene            complement(9377..>10537)
                     /gene="prfC"
                     /locus_tag="VGQ51_07935"
     CDS             complement(9377..>10537)
                     /gene="prfC"
                     /locus_tag="VGQ51_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219270.1"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV9036533.1"
                     /translation="RDGKNRFDLLEEIEKELNIQLHPMTWPINSGKDFKGVYNLYDKN
                     LVLFTANTKATDEDTLRFDDLGSGLLDERLGDKDAATLREDVELVDGVHGQLDVEDYL
                     AGKVAPVFFGSAINNFGVREMLDTFIRIAPTPRGRETSARPLEVEEDKFSGFVFKIHA
                     NLDPKHRDRIAFLRVCSGRFERNKYFHHVRLDKDVRFSNPYSFLARDKDVIEEAYPGD
                     VVGLFDTGNFKIGDTLTEGEDFFFTGIPSFSPEIFKEVVNKDPMKTKQLEKGLLQLTD
                     EGVAQLFTQFGGNKKIIGCVGELQFEVIQYRLLHEYGASVQFNAQPFYKACWITSRDP
                     KKLEDFTRFKHANIAEDKDGYLVYLAQSEWFLNTERTNNPDIEFHFTSEIHK"
BASE COUNT         2570 a         2930 c         2557 g         2480 t
ORIGIN      
        1 ccatggggaa agaatagatc agccttgaac ttcttcccga aaactcgtaa atatttcggt
       61 ccgtctgcac caggaaatgt cccgaagcgc tccggcaggc tgtcatggcg tttcgcatgg
      121 gactgctgcc gatagagtca atctccctga cctttccgcc ggcttccagc caatgaagcc
      181 ttgtctgctc caggatcagg atatccccat tcgcgtcctc cgcaaaatcc tcaacattct
      241 ctctcagccg aatacgccct aacatggaat cattgtcctg gttgtggatc attccgcggt
      301 aatagtaaca gacggacttg cgaaaaggcg ccatccacac cctgcctttg gaatccgtga
      361 acatcttcag cacctcgata tccggcagac catccgctac cgtaaaggtc ttgaaatggg
      421 tgccgtcaaa acggcttacc cccgtctcgg ttcccatcca taggaatccg tccttatcct
      481 gggtgatgca atagacgaca gaacccgcca gcccatcagc aatatcataa tgggtatagc
      541 tgtattcctg cgcgtaagaa gaaaaggaga tacagagaaa gagtacaata gacagcagcg
      601 gcgcgctttt catatcacct tataaaaata gcgaatcttt ttgggtaggc gagtgaaaaa
      661 cagggaactc cagcgtcacc cgcgtccccg cggcccggcc attgccgtcc tccatatccc
      721 tcacttcgac atgtatgctg tccctgtatt tcacgttcat catccggatg cggtctgccg
      781 tcagtgacat ccccttcgat tgatattcga tgtgttcgcc cgtcttgtag gcggcggctt
      841 tcttcctccc gataccattg tcttccacga tgaccatcaa cttctcatcc gatctcttga
      901 aaaggatcag aatagaaccg gtgccgtccg tcttatgacg caacccgtgc cgcatgctgt
      961 tttccacgaa gggctggatc agcataggcg gaatgacgaa gagttccata tcgatagaag
     1021 ggtcgatctc gactttgtaa tccatttttt ccttgaaccg caatttctcc aatgaaagat
     1081 aagtggatag gtaatcgact tcatcggcca gggatataaa attcctcgat gagttgtgca
     1141 aagtcgcccg gatcagcctg gcaaatccgg tcaggtatct gttggcggcc agggcttcat
     1201 gatcgaagat gtattgctga atggagttga ggcaattaaa gatgaaatgg gggttcatct
     1261 gggcctgcag tgccgtattc tccaattccg ccagccggtg actgacctgc tctttttcct
     1321 cctgacgacg atgaatacgt ttgatcctca tattgaacag caatcctaca agcagaacga
     1381 gaatcacaag gatcagtaca tagaaccagg cagcctgcca gaatggagtg gccacttcaa
     1441 cggacatttt cagcaatctg ctctctatcc cgaatttatt gatggcctgc agctgaaata
     1501 caaaatgccc cgagggaagg aaggggtatt ccaaaaaacc ttccttcgtc tcctgccagg
     1561 tagtgtccag ccccagcatc cggtagcggt atttgatatc gccgacactg cgataagaaa
     1621 tggcggcaaa ttcgaaccgg acatgtctgt cctggtaagg aataagaaga tcggccgaat
     1681 ccccgatcct atccttgccg gaattggcga ttgacagtag atgcagtctg caatccgaca
     1741 tcccattcac ctttgtctgg tcgaaatagc tcaggcccgc cggggtaccc acatatacca
     1801 cgggaccatc gacatagatc gtattgatga tatcggatcc gagcccatcc ttggaggtat
     1861 agtgaaggac gggatagcca gcgcgatcca gcgctatctt gttcaggcct ttatcggtgc
     1921 ccacccaaag tgtattctcc gcgatgaaca tcgttcgaca tatattactg ttcagaccgt
     1981 tgctcttatc gatcagggca atcacacggt tatcccgata tgcgatcacc cccccatcat
     2041 acgaggcgat ccaaagtgtc ccatcggaag attcgacgat gcccgaaatc cttctttgga
     2101 aaaagggatc tgtactaccc aaatagaccg acgaccggtc agggttcagc cgatagagac
     2161 cgttgagcgt gcctatataa caggtatcat ttctcgaaaa aatggtcgtg gtccgttcgc
     2221 gccaaagcgt gtcggttatc ttccattttc gggcatcgaa caaacacgcg ccccagccgg
     2281 cagctaccag gtattggtcc gccttcgttt tgaaaacagc ctttacaccc atcaggacgg
     2341 gttctccgta cctgctatgg gcaggtgtgc ggttgattcc gccgtccgtg gcaaggaaaa
     2401 ggccgccgga cgacatcttg tccagataca ggatccggct cctggcgcca aacaatccat
     2461 atgggacctt acgacggacg agaccttcgg tcgaaatgga aaaagtataa agcgtattga
     2521 agtcgtctcc taccactagt tcatccccta tccttctgaa ggcataaact ccggcttcct
     2581 gcatccccgg caacccgacc cggatcgtac ggaattcatc ggagttcaat cgataaatgc
     2641 catgccccat cgttgtaaac caaatgttgc ctgtggcatc tctgaatgtt ttcgacactt
     2701 cggtcccggg aaggaatcgc ttgtatttcc ccgtattcaa attgtacccg atcgtcccgg
     2761 tccgctcgtt cttgaagaac aggctgtctc ccacactgct aaaactgaca tgcatgtaat
     2821 atttcctgtt ccagttaagc cgggtgatct tgcctgacgc aaaagcagag atatcatagg
     2881 aattcaaatc ggtgtgccag atcattcctt tgggattcag ggaaatatag gttgacatcg
     2941 gagtcttgat atagaactgg cgaaatagcg cggaaccctt atccgacatc gtaaacacct
     3001 ttcccgcggt tgctaccagg aaattgccgg aatcgctacg gctgatcgct atcgccgatt
     3061 caatgggtcg cccgcccagc gagtcgatct ctcttaccgt tccatccgcc ccgatccaat
     3121 gcaaggccgg cttttccatg atcaggatat tgcccgccga gtcctccgca aaatcctcca
     3181 cattctctgt aagatgaatg cggctgagca tcggatcatt ttgctggttg tggatcttgc
     3241 cctggtaata ataacaaacc gatttgcgga aaggcgccat ccagaccctg cccttcgaat
     3301 caccgaacat ctccagtatc tcgatgtcgg gcagcccgtc ggcggcggtg aatgtcctga
     3361 agtgcgtgcc gtcaaaacgg cttacgcccg tctcggtccc cgcccaaata aaaccatctt
     3421 tgtcctgggt gatgcaatag acaacggaac ctgccagccc gtcggtaatg tcataatgcg
     3481 tatagctgta ttcctgcgcg aaggaggaaa agggcagatt tataaaaagg acacaggata
     3541 gcagccgggc gcatttcata tgaccttata aaaataaggc ttattgcgga tgatggtagg
     3601 gctcttgcga attactatcc ctacgttcct gcaatactgg cggaacttgg tccaggtata
     3661 acgttttttc ccggtgagcc cggcccacag catgcccagg aaaaagatgg ggatgttcac
     3721 tatatagcac aacagctgga aaaggaacca cccgactccg aattcttttc gaatcctcac
     3781 gaaattgcta agcagcagct gcaatccctt ccggtcgaaa atgttgacat atcccttgcc
     3841 atccgaccca aacgccaggt tggccgttac cccctgcaga tgaataactt ccagatcgcc
     3901 gtagatggcc atcttcccca gtttgcgaag ccggccgcac cactcggatt cctcggcata
     3961 caggaaaaaa tcctcgtcca tcatgcctgc cttgtcgatg gcctcctttt taaccatgag
     4021 gaaggcacca ttgatccagt ccacttcgat ctctttctgc tgatcgggca catgcggctt
     4081 tttgaccttg aaggtataac ccagccattt aaagaatgcg cccagatagg gcatcatcaa
     4141 caaatagttc acccccccct tcatggcata attacccgag atctggtggc tgccatccgg
     4201 attcagtaat tgtacccctg ccgcaacata gcccgagttc aggagacgcc ggtaacactc
     4261 ttcgatatct ttcccgcggg gcagcgtgtc cccgttcaat atcagcatcg cgtcgccccc
     4321 ggcgacgcgc atccccgcat tattgcctct tgcaaaaccg gcgttggaat ccagttgtac
     4381 ccactttacc tgggggaagg cccccaggat acgctcgcgg ctgtcgtcac cggaattgtt
     4441 gtcgacctct atgacctcga acgtgtatcc aacggggtct ctatagatct cggtcagaca
     4501 atcgatgtgc aattggggcg atttgaaatt gatgaggaca atacttagcg ccattaaaat
     4561 gatgcggtta aaaggttact aatataattc aggaacatga ccggttttga aaaaaaaccc
     4621 ttccgcagga accgcggggg gatccgtgac tgccagtcga tcatcctgcg caaaggctgt
     4681 ggtatcttct ctccattcac acattccttc aagtcgtcgc catcgcgaac ggcggcattc
     4741 cgcaatagcc gccaccattc atcgaagacc tttacggacc gcaaggtacg aatgccggcg
     4801 cggttcagcc accgcaattt ttcggagatc atcacctctc tcaggaaagg aacatcgtga
     4861 cgggaactga ctccttctgc gccaaaatcc gccacgatga gatcgatata gcggttccgg
     4921 acccgcggct cgttgaaaca gcggatattc aggtcccaat ccgcataagc cttatagcgg
     4981 atcctgaatc ccccgacgat cctgaatacg tctttcttat aaaaaaccgc ctgatgggga
     5041 atattcctgt gcattaactt ttccagcgtg aaagggccgt cataaacccc cttaaaagcc
     5101 gggcttacca cattgccata gaccatatcg aagtcactca caccgggtga ggaaaaaaaa
     5161 gtctccaaca catgttcatt gtgcagacgg tcgtcactac ccaggaaaaa gacccattct
     5221 cccctcgctt cggcgactcc cttgttcatg gcatcgtaca ctcctttatc cggcgaggat
     5281 tggacccgga cccgatggtc cttcgctgca tacccgcggg caatgtcgag ggtgttgtcc
     5341 cgggacacgc tgtccatgat aaggatctcg aagtcccgga atcgctgaca cagcacactg
     5401 tccagcgaat catgcagcgt tcgtccggcc tgcaatgtgg gtattattat ggttagacgc
     5461 gggctattca tgattccctc caaagtgcaa acgcattcct ccatcgggta agcctttttt
     5521 ttaggatccg ccagataaag ggggtcgtat ttttccttcc gggctgtccc tcgcttcgtc
     5581 ccctgtgatg atccagcaac tggaaataac ccgaattgta gtaaggtatg gccgcataat
     5641 agtccaccgc gtgttgccgg tattggtcga cacaattgaa ttcgttatcc acacggaagg
     5701 caggcatctt gtaatgattg ccgaattgag taggcatcct gaataatgga agacggtatt
     5761 tgagtgccag cagcgagaga acggactggt ctcttctatg ttctataaag tcggggagat
     5821 cccttttgcc ggaagtattg ggactatcag tcaggattcg cggatcgcgg caatattcga
     5881 gccattcgcc gacaaattgc atcgtcaggg gacatttgcg gaaaagaccg aaggccgcat
     5941 cgcaatgcgg gcctttccaa aaatagttgg aatcgcaccc catgagaata aagcaatccc
     6001 gtttggtcca ggcggcattg gtattgtccg cattgccgaa caggagcacg ggggtctgct
     6061 cccggcatag cgaaagcaac gggtccgggg aagcgatgat ctcgattccg ctgtccgaat
     6121 agatcacgat gtcgccatcc tgaacggtgt tgagcgcctc gcggatgata tacggcttcc
     6181 aaagccaata gcccatccct gtcggatgat cgagtatttc tttattggca gcgaaaaatg
     6241 gcgtctgacg gatctgttca aaatcgtacg atcggatatc gtcgataccg tatttagagg
     6301 cggaccggtt cagccgcatc ctgctttctt caaaaagctg attggacaga ttggtaagga
     6361 cgacgggcat gtcagaattc gaaatttgaa atgatgccta ccgaatgggc gccctcatgc
     6421 tgccctaccc atccgtttac ttccagcaga tagatatccc tgatgccata cgtttcatta
     6481 tatctccgga tgatcctgaa aaaggagttt ttcattttgc cgaccaggtc gtcccagacg
     6541 atgacgaacc gttctccgag cagacggtat tttaccagca tctcgaactc gaaaagaatg
     6601 gcctcggggg aatgcagggc atcggaaaag accaggttga acgaattgcc ttccagccgg
     6661 gcccagctgt tggcatccca aacatccccg gagacatagc ttaccttctt gcccccgaat
     6721 gaaaactcgc tgagcgagga ctggtttttt ttgaccgatc cggaaggcgt cgcccactct
     6781 gtcttcgact tcgcgacgag ccggctggcc agcaccgggt tgatctcctc gatatcgaag
     6841 gcggtgagac tggcctgtgg caacgttttc agcagctgga aaaagttctt tccgacggaa
     6901 atgccgatct cgagatagtt cacggaaccg aggtaacgat cggcgatgag tgtcatcaga
     6961 tcggtatagg tcggggcatc ccctatcggt ttattgatca cctccttgat agagtcgggt
     7021 acgccatagt tgaagaacga tcgtgcaaag gcctgctcat cgatccaatc gtctacctca
     7081 ccaaggacct tgcgattggc ccgcatatac ccggacagtt cggccggcga aaatgggatc
     7141 tctgatttat atgggatttt cttctgaaaa agctgcataa acgcatccag catagaactt
     7201 aaatttaagg cctgtaaaag tagctatttt tggacaaggg gtgccgcaga cacctgcaaa
     7261 tattggagga accgttcaag ccccccgcgt ttaagcggat cgagcgtata gctcaaatgc
     7321 tttgtgaagt aatcgttgag cgggaacgcc gggtaaggat tctcagcgac cacggcatcg
     7381 atatgcgcca aaccctgtct gttggcttcg tcgaattccg ccagaaagga agagtccagc
     7441 tttttgttgc tgatccacgc cgcaaacacg aaaggcagac cggtgagatc cttccaggct
     7501 ccggccagat cgtagatata gggtgaacgc aaccgctgct cgagcgcgcg gtcaccgatg
     7561 acgactcccg cggtcgtacc cctgatctgg ttccggaagt cggaagaggt agagaccagt
     7621 tccggttgga tcttccagta ttcccttagc agtatcttcg ccagctgcac agaggtcctg
     7681 ctctgataat ccagtaacag tctttcgatc cgttccatcg gggtctcgct gaagatacaa
     7741 accgaagcta catctccgtt actcccgata caatggttcc cattgatatg atattcgggc
     7801 aggaatggga tcaccgccac cgggactagc cccatatcga tctcgtcgcg caccagcatg
     7861 gaggcgatgc tggcaggatg gtcgatgatc agttcgatct gtttccggat agacgcccgc
     7921 tcgatcccat agagcagggg tttggtattc agatagctga cagctcccac tcgtattttt
     7981 ctgtccaatt cctttgcttt aattttggcg caaattatgg aattaaacgc acttactgcc
     8041 atttcaccgc tcgatgggcg ctatcggcat caggtccagc acctggacga ttatttttcg
     8101 gaatatgccc tgatccgcta tcgcgtgatc gtggaagtgg aatatttcct gttcctcggc
     8161 agaaagaagt tttttacact cgacgcaccc ggccggcgcg ccttacaggc catcgcggca
     8221 caattcagcc tggaagacgc gcaatggata aaggagaccg aaaagaccac caatcacgac
     8281 gtaaaagccg tcgaatactt tctcaaggac cggctgacca aagcgacccc gacagatgcg
     8341 gcattgggag aatggatcca ttttgcgctg acctcgcagg acatcaataa cactgccatt
     8401 ccgctgctct ggaaacatgc cgtcgaattc gaatatctgc cgggtctcct caatctgaaa
     8461 cgacagctgg aggtactggc ccgcgattgg aaagacattc ccatgctcgc ccggacgcac
     8521 ggacaacccg ccagccccac acttctcgga aaagagatcc ttgtttttat cgagaggctg
     8581 gaggaccagg tcgaactgct catccaggtt cctttctccg ccaaattcgg cggcgccacc
     8641 gggaacttca acgcccataa cgtggccttc cccggcaagg actgggttaa actcgctaac
     8701 gagttcgtcg aagggacgct gggtctccag cgacagcaat acacgacaca gatagagcac
     8761 tacgacaacc tggccgccca ttttgacgcc atcggccgga tcaataccat cctgatcgat
     8821 ctttgccgtg atctctggac ctatgtatcc atggattatt tcacccagaa aaccaaaaaa
     8881 ggagagattg gctcatccgc catgccgcat aaggtcaatc ccatcgattt tgaaaatgcg
     8941 gagggcaatc tgggactggc caacgcgctg ttcggacatt tctccgccaa actgcccata
     9001 tccagactac aacgcgacct caccgactcg acggtgttgc gcaatatcgg cgttcccttc
     9061 gcccatacca tcctggcgtt gaattccatc ggaaaaggac tggacaaact ggtgctcaac
     9121 cgcgcccgtc tgcagcaaga cctcgagaac aactgggccg tggtcgccga agccattcaa
     9181 accgtcctgc gacgggagaa ctatccgaaa ccctatgagg cattgaaaga gctgacgcgc
     9241 gggaaatcgg ggatcaccaa acagtccctt cataaattca tcgacgggct gaaagtatcg
     9301 gctgcggtga aaaaagagct caaaaaggtc acgccacaca actacacggg catctcgtat
     9361 taggggcagg gcctctttac ttatggatct cggacgtaaa atggaactcg atatccggat
     9421 tattcgtccg ctccgtattc aggaaccatt cgctctgcgc cagatacacc agatacccat
     9481 ctttgtcttc cgcgatattc gcgtgtttaa atcgggtaaa atcttccagt ttcttcggat
     9541 ccctgctcgt gatccagcaa gccttataaa aaggctgggc gttgaactgg accgaagcgc
     9601 cgtattcatg cagcagccgg tattggatca cttcgaactg aagctctccc acgcatccga
     9661 tgatcttctt attcccgcca aactgggtga acaactgcgc cacgccttca tcggtcaact
     9721 gcaataaccc cttctccagc tgtttggtct tcatcgggtc cttattgacc acttccttga
     9781 atatttccgg ggagaaagaa gggatgccgg tgaaaaagaa atcctctccc tccgtcagcg
     9841 tatccccgat cttgaaattg cccgtatcga acagacccac cacgtcgccg ggataggctt
     9901 cctcgatcac atccttatcc cgcgctaaaa agctataggg gttgctgaat cgaacgtctt
     9961 tgtccaaccg cacatgatgg aaatatttgt tccgctcgaa ccggccgctg catacccgga
    10021 gaaatgcgat cctgtcccgg tgcttggggt cgagattggc gtggatctta aacacaaaac
    10081 cgctgaactt atcttcttcg acctcgagcg gacgagcaga agtctcgcga ccgcggggcg
    10141 ttggagcgat ccggataaag gtatccagca tttccctgac gccgaaattg ttgatggcgc
    10201 tgccgaagaa caccggcgcc acctttccgg cgagatagtc ctcgacgtcc agctgaccat
    10261 ggacgccatc gaccagttct acatcctcgc gcagcgtagc cgcatcttta tcgccgagac
    10321 gctcatccag caacccgctg cccagatcat cgaagcgcag cgtatcctca tcggtagcct
    10381 tggtattggc cgtgaatagg acgagattct tgtcgtatag gttataaaca cccttgaaat
    10441 cctttccgct gttgatcggc caggtcatgg gatgcaattg tatattcagc tccttttcga
    10501 tctcctcgag cagatcgaac cggttcttcc cgtcccg
//
LOCUS       DASYSV010000258         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1968267, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000258 DASYSV010000000
VERSION     DASYSV010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968267"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="VGQ51_07940"
     CDS             complement(<1..1605)
                     /locus_tag="VGQ51_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEV9036534.1"
                     /translation="MRIPYLLLLFVLPAACTAQDRRPTPQPSYVKDNFDKREVDIPMR
                     DGIRLHTVIYTPKDNTRAEPFLMQRTPYSAGPYGDSVSRGNLGPNSELMRSGYIFVYQ
                     DVRGRYMSEGDFQEMTPAGGHTDESTDTWDTIDWLLKNIKNNNGRVGIWGISYPGFYA
                     SASLPNAHPAIKAVSPQAPVTDEFIGDDANHNGAFFLLDNFDFDNGFDIPRPTPVKHY
                     AGPIFHVSINDAYRFFLDLGPLKNSNNADYFNHRGKIWDEYLAHSTYDSYWQARNIRT
                     HLKNIRPAVLVVGGWFDAEDMFGSLRTYEAIEKQSPHNDDHIIMGPWTHGAWSAKTWN
                     HFGPLDFGENVNIHYHEIETAFFNHYLKDSAQAPLAEATVFETGSNQWKQYATWPPAE
                     ATPTKFDLQSGGGLDAEKPSTGYDEYVSDPSNPVPYINGVHGGRDNTYIVTDQRFAAQ
                     RPDVLSYQTPPLKKDLTVTGRLKADIFLSSTGTDADLIVKLIDVFDSTGYQRLVRAEV
                     FRCKFRNSFEKPEPLIPNKPAEIAFNM"
     gene            complement(1611..>2928)
                     /locus_tag="VGQ51_07945"
     CDS             complement(1611..>2928)
                     /locus_tag="VGQ51_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEV9036535.1"
                     /translation="DFHHNGAFFVMDGWAFYSSFGKPRPKPTTIGPSGFDYYTKDNYK
                     WYLETGALKNIMKLTGDSIAFWTDLYQHPNRDAWWQARNARVALYDIKPAMLVVGGLF
                     DAEDCFGAQNVYKALKKQSPSTNSHLVLGPWYHGQWASNDGTHMGNVRFGSNTSWWYQ
                     NNIEIPFFEYYLNGKGPEPHLPAATVFVTGADEWRQFDQWPPAQSQDQTIYLQEDGGL
                     AWTAPTGKTSFSEYISDPAHPVPYADGVHFFRTREYMDDDQRFTSRRADVLTFQTPVL
                     QEDLTLAGPVTADLKVSLTGTDADFVVKVIDVFPDDFSYTAQGPVPVRSHDNAPGYPM
                     GGYEMLVRAEIMRGRFRESFEKPTPFTPGKVETVKWTTPDVAHTFKKGHRLMIQIQSS
                     WFPLVDRNPQKFVDIYTANDSDFQKETIRVFHDAAHPSAITLPVLK"
BASE COUNT          578 a          693 c          960 g          697 t
ORIGIN      
        1 catgttgaag gcgatctccg ccggcttgtt tgggatgagg ggttcgggtt tttcaaagct
       61 gttgcggaat ttgcaacgga agacttcggc gcgaacgagt cgttggtagc cggtggaatc
      121 gaaaacatcg atcagcttga cgatcagatc ggcatctgtg ccggtggagg agaggaagat
      181 atcggctttc agacggccgg taaccgtgag atctttttta agcggcggcg tttgatagga
      241 aaggacatcc ggcctttgcg cggcgaagcg ctggtcggtg acgatgtagg tgttgtcacg
      301 gccgccgtgt acgccgttga tatagggaac ggggttggag gggtcgctga cgtattcgtc
      361 atagccggtg gaaggttttt cggcgtcgag gcctccgccc gactggaggt cgaatttcgt
      421 gggtgttgct tcggcaggcg gccaggtagc gtattgtttc cactggttgg agcccgtctc
      481 gaaaacagtg gcttcggcga ggggtgcctg tgcagagtct ttgagatagt gattaaagaa
      541 ggcggtctcg atctcatgat aatgaatgtt cacattttct ccgaagtcca gggggccgaa
      601 atggttccag gttttcgcgc tccaggcacc gtgcgtccag ggtcccatga tgatatgatc
      661 gtcgttgtgc ggcgactgtt tttctatcgc ctcgtaggtg cgcagggaac cgaacatgtc
      721 ctcggcatcg aaccagccgc cgactaccag tacggcgggg cggatattct tcagatgggt
      781 gcggatattg cgggcctgcc aataggaatc gtaggtggaa tgcgcgaggt attcatccca
      841 gatctttccc cggtgattga aataatccgc attgttgctg ttcttcaacg ggccgagatc
      901 gagaaagaaa cggtaggcgt cgttgatact cacgtggaag atggggcccg catagtgttt
      961 gacaggggtg gggcggggga tgtcgaaacc attgtcgaaa tcgaaattgt cgagaaggaa
     1021 aaaggctccg ttgtggttgg cgtcgtcgcc gatgaattcg tcggtgacgg gcgcctgggg
     1081 tgaaacggcc ttgatagcgg gatgtgcgtt gggcagggag gcggaggcgt agaaaccggg
     1141 ataggagatg ccccagatac cgacgcggcc gttgttgttt tttatgttct ttagcagcca
     1201 gtcgatggtg tcccaggtgt cggtgctctc gtcggtgtga ccgccggcgg gcgtcatttc
     1261 ctggaaatcg ccttcgctca tatatcggcc acggacatcc tggtaaacga agatatatcc
     1321 gctgcgcatc agctcactgt tcggtcccag gtttccccgg cttacggaat caccgtaggg
     1381 tcccgccgaa tacggcgtcc gttgcatgag gaagggttcg gcgcgggtat tgtcctttgg
     1441 cgtgtagatc accgtatgga ggcgaatgcc gtcacgcatg gggatgtcga cctcgcgttt
     1501 gtcgaagttg tctttgacat agctgggctg gggcgtcggg cggcggtcct gggcggtgca
     1561 ggcggcgggc agcacgaaaa ggagcaatag ataaggtatg cgcatagagg tcattttaaa
     1621 actggcaggg taatggcaga aggatgtgcc gcgtcatgga aaacgcggat ggtctctttc
     1681 tggaaatcgc tgtcgttggc tgtataaata tccacaaact tttgcggatt ccggtcaacc
     1741 agcgggaacc agctgctttg gatctggatc atgagtcggt gtcctttttt aaacgtatga
     1801 gccacgtcgg gagtggtcca ttttactgtc tccaccttgc cgggtgtgaa gggggtgggt
     1861 ttttcgaagc tttccctgaa ccggccgcgc atgatctcgg cgcggacgag catctcgtag
     1921 ccgcccatgg gataaccggg ggcattgtca tggcttctta ccggcaccgg tccctgagcc
     1981 gtatagctga aatcgtctgg gaatacgtcg atgaccttga cgacgaaatc ggcgtcggtt
     2041 ccggtgaggc tgaccttcag atcggcggtg acgggtccgg ccagggtcag gtcttcctgc
     2101 aataccggtg tctggaaggt gagcacgtcc gcccggcggc tggtaaagcg ctggtcatcg
     2161 tccatatatt cgcgggtgcg gaagaagtgg acgccgtcgg catagggaac cggatgggcg
     2221 ggatcgctga tatattcgct gaaactggtc ttgccggttg gcgccgtcca tgccagtccg
     2281 ccgtcctcct gcaggtagat agtctggtcc tgcgactgtg cggggggcca ctgatcgaat
     2341 tgtctccatt cgtcggcgcc ggtgacgaag acggtagcgg cgggcaggtg gggttcggga
     2401 ccctttccat tcaggtagta ttcgaagaaa ggtatctcga tgttattctg gtaccaccag
     2461 gacgtattgg agccgaaccg gacgttgccc atgtgggtcc cgtcgttgga cgcccattgt
     2521 ccgtggtacc agggaccgag gacgagatga gagttcgtgg aaggactttg ctttttcaat
     2581 gctttgtaca cattctgcgc accgaagcaa tcctcggcat cgaagagacc acctacgacc
     2641 agcatggccg gtttgatgtc gtagagcgcg acgcgggcat tacgtgcctg ccaccacgcg
     2701 tcgcggttgg gatgctggta caaatccgtc cagaaagcga tgctgtcgcc ggtgagcttc
     2761 atgatatttt tcagtgcgcc cgtctccaga taccatttat aattatcctt tgtatagtag
     2821 tcgaaaccgg aaggtccgat ggtggttggt ttcggtcgcg gtttgccgaa agaggaatag
     2881 aaagcccagc catccatgac gaagaaagcg ccattatgat gaaaatcg
//
LOCUS       DASYSV010000259         9245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1200980, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000259 DASYSV010000000
VERSION     DASYSV010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200980"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VGQ51_07950"
     CDS             complement(<1..668)
                     /locus_tag="VGQ51_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV9036536.1"
                     /translation="MTGKKDITIYDIAKVLGLSPATVSRGLKDHPAINIKTRKRIMDM
                     AKEMGYRSNSFASNLRMQKTHTIGIIVHELRSQFISSVLAGIEKVATDAGYDLIIGHS
                     SETWRKEAANAHNLFHKRVDGLIASLAFDTKEMDHFDPFVQKGIPIVFFDRVEEFSYG
                     TRVIIDNVKAGYEATAHLAAQGCRRIAHITASLARNVYAGRLKGYQQALSDYGLPYDP
                     SLLIT"
     gene            834..2360
                     /locus_tag="VGQ51_07955"
     CDS             834..2360
                     /locus_tag="VGQ51_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEV9036537.1"
                     /translation="MLLLGIDLGTSSVKVAVTDAQTHQLLATAQFPDTEAPILSRQPG
                     WAEQSPETWWQQVKQAIARVNATGFYDPQDIAAIGIAYQMHGLVLVDKDHRVLRDSII
                     WCDSRAVPYGDRAFDTIGIRRSLERLLNSPGNFTAAKLAWVKDREPQIYERIDKVLLP
                     GDFIAMRFTGEATTSISALSEGIFWDFRADRLSEDILKTFGFDKQVFPEIRPVFSAHG
                     GLTEPVARELGLKKGIPVTYKAGDQPNNALSLNVLEPGEVAATAGTSGVIYGVSDELT
                     YDPGSRVNSFAHVNYTPKDRRVGVLLCINGTGILNSWIRDLVGGIGAIKGGGILEGGN
                     VGNWNYERLNEVASKVPAGSAGLKVLPFGNGAERIFHNKIIGGQLLNIDLNVHGPAHV
                     YRAGQEGIAFSFRYGLDIMRENGMNPGIIRAGKANMFRSNVFVEAFVNATGVPVELYN
                     SDGSVGAALGAGIGAGIFRNGKEAFAGLRPLHLIEPTKAEQYETLYGEWKKLLQSFIN
                     "
     gene            2366..3694
                     /gene="xylA"
                     /locus_tag="VGQ51_07960"
     CDS             2366..3694
                     /gene="xylA"
                     /locus_tag="VGQ51_07960"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEV9036538.1"
                     /translation="MPVITGQKEFFKGIGPIKYEGTGTDNPLAYRWYDADKVVAGKPM
                     KDHLRMAVAYWHSFCGNGADPFGSPTHLFPWNEQSDAVGRAKDKMDAAFEFITKLGLP
                     FYCFHDVDVVDYTNDVADNEKRLQKLVDYAKQKQAASGVKLLWGTANLFSNMRYMNGA
                     ATNPDFHVLAHAGAQVKAALDATIALDGANYVFWGGREGYMSLLNTNMKREQEHLAKF
                     LHTAKDYARKQGFKGVFFIEPKPCEPTKHQYDYDSATVIGFLRQYGLLEDFKLNIEVN
                     HATLAGHTFTHELQVAVDAGMLGSMDANRGDYQNGWDTDQFPNNINELVEAMLIILES
                     GGFGGGGINFDAKIRRNSTDPADLFHAHIGGIDTFARALVVADAVLARSEYKKLRAAR
                     YASYDSGAGAAFEKGGVSLEDLREYAIAQGEPAVISGRQEYLENLINRYI"
     gene            3719..5365
                     /gene="xylE"
                     /locus_tag="VGQ51_07965"
     CDS             3719..5365
                     /gene="xylE"
                     /locus_tag="VGQ51_07965"
                     /inference="COORDINATES: protein motif:HMM:NF007484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HEV9036539.1"
                     /translation="MIDTSAKTAGSVTAKVSQTYITLLTIVAALGGLLFGYDTAVVNG
                     AEKSLTAFFITPILDAARSEYAVRMITQYRILLIVVLFIVGLVICAQIVRLWGGKKGG
                     LVSIVLLALLIVWAVSFSGTPVSLAAADLQDTANAIKGFLIASALIGCVIGGASAGFI
                     SKSFGRKNGLVISAVCFFVSAIGAWKPEAFNFFGTLDAYSFVVYRIIGGIGVGIASMI
                     SPMYIAEIAPARVRGRLVSFNQLAIIFGMLVIYFVNYYIARQGDEQWLTTVGWRWMFF
                     SGVIPAGVFFILLFFVPETPRYLVMKGHDEKALKVLARITGGAQAEGVLKEIRGTLQE
                     KNAPWLSYGFFLIFVGILLSVFQQFVGINVVLYYAANIFRDMGASTDSSLLQTIIVGV
                     VNLLFTVVAILTVDKFGRKPLMIIGSIGMAVSMFALGLSFYTQHIGLAALIFMLVYTA
                     AFAMSWGPVCWVLLAEIFPNSIRSALSIAVAAQWIANWVVSLTFPMMNDNGWLTGKFH
                     HGFSYWIYGVMGVLSALFVWKMVPETKGKTLEQIEHIWKR"
     gene            5647..8544
                     /locus_tag="VGQ51_07970"
     CDS             5647..8544
                     /locus_tag="VGQ51_07970"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV9036540.1"
                     /translation="MQTPKKTIILPSGGSTPAVGLLLNFSQEKRGGLAVELLLVIEKK
                     EGRSYQRIPLAGGHYKAYLQGFAPPLAAVIRRLSDEALLDHLVQNGFGWLRDADKPFD
                     RLDERHYLLLRQWTSAALAELKPLAPSVRHLGYLAPAAAFVSPNIRPAAISAATPELE
                     FRLERQLGRLVFLCSVRLGEALFPLDDFQIFSTFLKSGNEFFLLRAADLELLMRYKAG
                     ELETKEEGLPRFMATVVRPLAERYKVNVDAVIITETIDVEPRSRIYVSELNENFLLIK
                     PKWEYAGHEVEETDEAETKVETENRVYRIVRRKADEARLTDALKGLHTRFEGQANGYF
                     YLTFKEAQEKSWFIRMYQLMQQMDVPVLGMNKLRKFKYNTNVPVFEIVAGRSIDWFDL
                     TIRVSYGDLVVPLAELRKAILGRQDFILLSDGSLGMLPKEWIERYSLLLKMGQVREGG
                     MRLSAVHWGVMAEAGGAESLQAELEEKKRRWAGFDAGKMRVVPAAVQAKLRDYQVAGF
                     RWMCLLDEMGWGGVLADDMGLGKTLQTLSFLQHVVERYPEETHLVVCPTSLLYNWEGE
                     LKKFVPGLDYHLHYGAGRELDEGTFRRAHVIITSYGMVRNDIAHFAGHTFGYVILDES
                     QAIKNPASQVRKAVQELRARNRLALSGTPVQNNTFDLYAQMDFLNPGMLGTAEFFRSE
                     FANPIDKNGDREVAARLRKLVYPFLLRRTKEQVAGDLPDRTEMVLWCEMGDEQRKIYN
                     SFKEHYRESLLERIAADGMGRSNIYLLEGLTKLRQICDSPALLKEEAAVGGSYPNASI
                     KMEELVREIEENTGDHKALVFSQFATMLRLIGSELESRGIPYLYLDGGVPAEERKTLV
                     RQFQEEDAARVFLISLKAGGVGLTLTAADYVYLVDPWWNPAAEQQAIDRSHRIGQVRK
                     VFAYRMICKDTVEEKILQLQQKKKTLAEDLVGDESGFVKKLTAEDVAYLFS"
     gene            8563..>9245
                     /locus_tag="VGQ51_07975"
     CDS             8563..>9245
                     /locus_tag="VGQ51_07975"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEV9036541.1"
                     /translation="MNWSTFLRTLLEVADKYYILAGIAFLVYYVLLRRRSSGKKIQPR
                     FPGAKDYQREVVFSTISILIFAMPPLLLVHTDALRPHTTFYKDIRLHSRWYFFGAFPL
                     LFLMHDAYFYWMHRLIHHPRLFRLFHLVHHRSVNPSPWAAYAFHPLEAFLESLIFVIF
                     LFTIPINVWHLFFFFLISLAYNVYGHLGFELYPSGFNRHWLGKWVNTSVSHNQHHRHF
                     KGNYGLYFL"
BASE COUNT         1988 a         1943 c         3064 g         2250 t
ORIGIN      
        1 gtgatgagca gcgaggggtc gtagggcaga ccatagtcgg atagcgcctg ctgataacct
       61 ttcagacgtc ctgcatatac atttctcgcg aggctggccg tgatgtgggc gatgcggcga
      121 cagccctggg ccgcgaggtg ggcggtggct tcgtagcctg cctttacgtt atcgatgatc
      181 acgcgggtgc cgtaggagaa ttcttcgaca cgatcgaaga acacgatggg gatgcctttt
      241 tgcacgaaag ggtcgaagtg gtccatctcc tttgtgtcga aggcgagaga ggcgatgaga
      301 ccgtccacgc gtttgtgaaa gaggttgtgg gcgttagctg cttcctttcg ccaggtttcg
      361 gaggagtgac cgatgatgag atcgtagcct gcgtcggtgg cgaccttctc gatgcctgcg
      421 agcaccgagc tgatgaattg gcttcggagc tcgtgaacga taattccgat ggtatgcgtt
      481 ttttgcatcc ggaggttgct ggcgaagctg ttggagcgat agcccatttc tttggccatg
      541 tccatgatgc gtttgcgtgt cttgatattg atggcggggt ggtcttttag gcccctgcta
      601 acggttgccg gtgagaggcc gaggactttc gcaatatcgt agatcgtaat atcttttttt
      661 cccgtcataa agtttggtag gttagtggtt tggaccgcct aaaattaagg attttaaaaa
      721 aattaatgca atcggttaca atttggaaaa acggtcctat atttacccgc cggctgcggt
      781 cgtgagcgta ataggcaaac gacgcagctg cagccggcaa aataattctt ttcatgctat
      841 tattaggaat cgatctgggg acttcctctg taaaggtcgc ggtgacggat gcgcagacgc
      901 atcaattgct ggcgacagct caatttccgg acacggaggc tcctattcta tcgcggcagc
      961 cgggttgggc ggagcagtcg cctgagactt ggtggcagca ggtgaaacag gcgatcgcga
     1021 gagtgaatgc gacgggcttt tatgatccgc aggatattgc ggcgataggg atcgcgtatc
     1081 agatgcatgg attggtattg gtggacaagg atcatcgtgt gctccgcgac agcattatct
     1141 ggtgtgatag tcgtgcggtg ccttatggcg accgtgcttt cgatacgatc gggatccgca
     1201 ggagtctcga acgcctgctc aattcacccg ggaattttac ggcggcgaag ctggcgtggg
     1261 tgaaggaccg cgaaccgcag atctatgagc ggatcgacaa ggtcttgttg ccgggggatt
     1321 ttatcgccat gcggtttacg ggagaggcaa ccacttctat ctccgccttg tcggaaggta
     1381 tcttttggga tttcagggcg gatagattgt cagaggatat tttaaaaacg tttgggttcg
     1441 ataaacaggt atttccggag atacggccgg tgttcagcgc gcatggcggt ttgacggaac
     1501 ccgttgcgcg tgagctggga ttgaagaagg gaatacccgt gacctataaa gcgggtgatc
     1561 agccgaacaa tgcgctttcg ttgaacgtgc tcgagcccgg tgaagtggcg gctactgccg
     1621 gcacctcggg tgtcatttac ggcgtgagcg atgaattgac ctatgacccc ggaagccggg
     1681 tgaacagttt tgcgcatgtc aactatacgc cgaaggacag gcgcgtgggt gtgctgttgt
     1741 gtatcaatgg aacgggtatt ctgaatagtt ggatacgcga cctcgtgggc ggtatagggg
     1801 caataaaagg cggaggcatc ctcgaaggcg ggaacgtggg gaattggaat tacgagcgac
     1861 tgaacgaagt cgcatcgaag gtgccagcgg gcagcgcggg tttgaaggtg ttgcctttcg
     1921 gcaatggtgc ggagcgcatt ttccacaaca agatcatcgg ggggcaatta ctgaatatcg
     1981 atctgaatgt gcatggtccg gcgcatgttt atcgcgcggg tcaggaaggg attgcgtttt
     2041 cttttcgata cggcctcgat atcatgcgcg agaatggcat gaaccccggg ataatcaggg
     2101 ctgggaaggc gaatatgttc cggagcaatg tgttcgtaga ggcgtttgtg aacgcaaccg
     2161 gggtgcccgt ggagctgtac aatagtgacg gaagcgtggg ggcggcattg ggagcgggca
     2221 tcggggcagg catcttccgg aacgggaagg aagcctttgc ggggttgaga ccgctgcacc
     2281 tgatcgagcc tactaaggcg gagcaatatg agaccttgta tggggaatgg aaaaaacttt
     2341 tgcaatcatt cataaactaa aagctatgcc agttattacg ggacagaaag aattttttaa
     2401 ggggatcggt cccataaagt acgaggggac gggaaccgac aatccgcttg cgtatcgttg
     2461 gtatgatgcg gataaggtag tggccggaaa gcccatgaaa gaccatctgc ggatggccgt
     2521 agcctattgg cattcgttct gtggcaatgg tgcggaccct ttcgggtcgc cgacgcatct
     2581 tttcccctgg aatgagcaaa gcgatgcggt tggccgggcg aaggacaaaa tggatgcggc
     2641 gttcgaattc atcacgaaac tggggttgcc tttctattgt tttcacgacg tggatgtagt
     2701 ggattacacc aatgatgtag cggataatga gaagcggttg cagaagctgg tagactatgc
     2761 aaagcagaag caggcggcca gcggagtgaa gttgttgtgg ggtaccgcga atcttttttc
     2821 gaatatgcgt tatatgaacg gcgccgccac caatccggat ttccatgtgc tcgcgcatgc
     2881 gggtgctcag gtgaaggcgg cattggatgc aacgatcgcg ctcgatggcg ccaactatgt
     2941 attctggggt gggcgtgaag ggtatatgag cctgctgaat acgaatatga aacgggagca
     3001 ggagcatctg gctaaatttt tacataccgc aaaggactat gcgcggaagc agggtttcaa
     3061 gggcgtgttc tttatcgagc cgaagccgtg cgagccgacc aagcaccagt atgattacga
     3121 cagtgcgacc gtcatcgggt ttttgcggca gtatgggttg ctggaagatt ttaaactgaa
     3181 tatagaggtg aaccatgcga cgctggcggg tcacaccttc acgcatgagc tgcaggtagc
     3241 ggtggatgcg ggaatgttgg ggtcgatgga tgccaatcgc ggggattatc agaatggatg
     3301 ggatacggat cagttcccga acaatatcaa tgagctggtc gaagcgatgc tgatcatact
     3361 ggaaagcggc ggtttcggcg gcggggggat caatttcgat gcgaagatcc gtcgtaactc
     3421 gaccgatccg gcggatctgt tccatgcgca tatcggtggg atcgacacgt ttgcgcgggc
     3481 gctggtggtt gcggatgctg ttttggcgcg atcggaatac aagaagctgc gggctgcccg
     3541 ctatgcgtcg tatgacagcg gcgccggcgc cgcgttcgag aaagggggag tgagcctgga
     3601 ggatctgagg gagtatgcga ttgcgcaggg cgaacctgcg gtgatcagcg ggcgccagga
     3661 atatttggag aatttgatca acagatatat ttaaactact aattgcttcc atatggtaat
     3721 gatcgacaca tccgcgaaaa cggcgggctc cgtcacggcg aaggtgagcc agacgtatat
     3781 cacgctgctg accattgtgg cggcattggg tggcttattg ttcggttacg acaccgccgt
     3841 cgtgaacgga gcggaaaaat cgctgaccgc cttttttata acgcctatac tggatgccgc
     3901 gcgttcggag tatgccgtga ggatgatcac gcaatacagg atcttgttga tagtggtgtt
     3961 gttcatcgtg gggctggtga tctgtgcgca gatcgttcgg ctatggggtg gtaagaaggg
     4021 gggccttgtg agtatcgtgt tgctggcttt gctgatcgtg tgggcggtgt cgttctcggg
     4081 aacgccggtg tcgctagctg ctgccgatct acaggatacc gccaatgcca ttaagggatt
     4141 tctcatagcc agcgcgttga tcgggtgtgt gatcggcggg gcgtcggcgg gatttatttc
     4201 aaagtcattc ggccggaaga acgggctggt gatatcggcg gtatgtttct tcgtctcggc
     4261 gatcggcgca tggaaacccg aggcctttaa tttttttggg acgctggatg cgtattcatt
     4321 cgtggtgtat cgcattatcg gggggatcgg tgtgggtata gccagcatga tctctccgat
     4381 gtatattgcc gagatagcgc ctgcgagggt acgcgggagg ctggtatcct tcaaccagct
     4441 ggcgatcatc ttcggcatgc tggtcattta tttcgtcaat tattatatcg cccgacaagg
     4501 ggatgagcaa tggctgacaa ccgtcggttg gcgctggatg ttcttttccg gtgtcattcc
     4561 tgcgggggtt ttcttcatac tgttgttctt cgtgccggag acgccgcgtt acctggtcat
     4621 gaaggggcat gacgaaaaag cgttgaaggt gttggcgcgg atcacgggag gcgcacaggc
     4681 agagggggta ttgaaggaga tccgagggac gctgcaggag aagaacgcac cgtggttgtc
     4741 atatgggttt ttcttgatct tcgtggggat attgctttcg gtgttccagc aattcgtggg
     4801 gatcaacgta gtgttgtact atgctgccaa tattttcagg gatatgggcg cgtcgaccga
     4861 cagctcgctt ttgcagacga tcatcgtggg cgtggtgaat ttgttgttca cggtggtcgc
     4921 gatactcact gtcgataagt ttggacggaa accgttgatg atcatcggat cgatcggtat
     4981 ggcggtgagc atgtttgcgc tggggctgtc gttctatacg cagcatatcg ggcttgccgc
     5041 gctgatcttt atgttggtgt atacggccgc gtttgcgatg agctgggggc cggtgtgctg
     5101 ggtcttgctg gcggaaatat ttcccaatag catacgtagc gccctgtcga tcgccgtggc
     5161 ggcccaatgg atcgccaatt gggtggtttc gttgactttc cctatgatga atgacaatgg
     5221 gtggttaacg ggcaagttcc atcatgggtt ttcgtattgg atctatgggg tgatgggagt
     5281 tttgtcggcg ctgtttgtct ggaagatggt gcctgagacg aagggaaaga cgctggagca
     5341 gatagagcat atctggaaga gatagcgcgg cgcgcgcaaa agggaaatgg agcgccgggt
     5401 gctggacagg cgcacggagg cggcggggca ggcacacgga ggcggcgggg caggcacact
     5461 gaggcggcgg gcagacacac tgaggcggcg gggcaggcgt taacggcagc aatctttttt
     5521 acggataaag atggccttga acaggcggac gaaaatattt ttgatcgatc tcatcgaatt
     5581 tcgggtggag caaagttcat tatttttgcg ggcggtctcg ttgtccttta caaataatta
     5641 acaaaattgc agacacccaa gaaaacgatc atcttaccca gcggcggaag cacaccggct
     5701 gtgggactat tgttgaattt tagccaggag aagaggggag gattggcggt cgagctgtta
     5761 ctggtcattg aaaagaagga aggaaggagc taccagcgca ttccgctggc gggtggtcat
     5821 tataaagcct atttgcaggg gttcgccccg ccactggccg ccgttatcag acgcctgtcc
     5881 gacgaggcgt tgctggatca tctcgtacaa aatgggttcg gctggttgag agatgcggac
     5941 aagcccttcg acaggctcga tgaaaggcat tacctgctgt tgcggcaatg gacgagcgcg
     6001 gcgctggccg aattgaaacc gttggcgcct tccgtccggc acctggggta tctggccccg
     6061 gcggcagcct ttgtttcccc gaatatccgg ccggcggcta tcagcgcggc cactccggaa
     6121 ctggaattcc ggctggaaag acagttggga agactggtat tcctttgctc ggtgcgtctc
     6181 ggcgaggcgc tttttccgct ggacgacttc cagatattct ccacctttct gaagagcggt
     6241 aatgaattct ttctattgcg ggcagcggac cttgaactat tgatgcggta taaggccgga
     6301 gaactggaga cgaaggaaga aggtttgccg cgttttatgg cgacggtggt ccggccgctg
     6361 gcggaacgtt ataaggtgaa tgtggatgcc gtgatcatta cggagacgat cgacgtggag
     6421 ccacggagcc ggatctatgt cagcgaactg aatgagaatt ttctattgat caaacccaaa
     6481 tgggaatatg cggggcatga agtggaagag accgatgagg cagagacgaa ggtggagacg
     6541 gagaacaggg tttaccgtat cgttcgccgg aaagccgatg aggcgaggtt gacggacgcg
     6601 ttgaagggat tgcatacgcg gttcgaaggg caggcaaacg gttattttta tctgactttt
     6661 aaggaggcgc aggagaaaag ctggtttatc cggatgtatc agctgatgca gcagatggat
     6721 gtgcccgtgt tgggcatgaa taagctccgg aaattcaaat acaatacgaa tgtgcctgtg
     6781 ttcgagatcg tggcggggcg atcgatcgac tggttcgatc tgacgatccg tgtcagttat
     6841 ggggacctgg tggtgccgct ggctgaattg cggaaggcta ttttgggtcg gcaggatttt
     6901 atccttttgt ccgatgggag tctggggatg ttaccgaagg aatggatcga gcgatattcg
     6961 ttattgttga agatgggaca ggtgagggag ggcggaatgc ggctgtcggc ggtgcattgg
     7021 ggggtgatgg cggaagcagg gggcgctgag tcgttgcagg cggagttgga agaaaagaaa
     7081 agacggtggg cggggttcga tgccgggaaa atgcgggtgg tgccggcggc ggtgcaggcg
     7141 aagcttcggg attaccaggt ggcgggtttc cgttggatgt gtctgctgga tgaaatgggg
     7201 tggggtggcg ttctggccga cgatatgggg ttgggtaaga ccttgcagac cttgagtttc
     7261 ctgcaacatg tcgtggagcg ttatccggag gaaacgcatc tggtcgtatg tccgacatcg
     7321 ttgttgtata actgggaagg ggagttgaag aaattcgttc cggggctgga ttatcatctg
     7381 cactatggcg ccgggcggga gctggatgag ggaactttcc ggcgggcgca tgtgatcatc
     7441 accagctatg gcatggtccg caacgatatc gcgcatttcg cggggcatac gttcgggtat
     7501 gtgatcctgg acgagagtca ggctatcaag aacccggctt cgcaggtgcg gaaggcggtg
     7561 caggagctgc gcgcgcgtaa ccggctggcg ttgagcggga cgcctgttca aaataatacg
     7621 ttcgatcttt atgcgcagat ggattttttg aacccgggga tgctgggaac cgccgaattt
     7681 ttccgcagtg aatttgccaa cccgatagac aagaacgggg acagggaggt tgccgcgcgg
     7741 ttgcggaagc tggtttatcc ttttctgtta cgaaggacga aggagcaggt ggcgggtgat
     7801 ctgccggacc ggacagagat ggttttgtgg tgtgaaatgg gggatgagca gcggaagatc
     7861 tataattctt ttaaagagca ttaccgtgaa agtctgttgg agcggatagc ggcggatggg
     7921 atgggaagga gcaatatcta tttattggag gggttgacga agctgcggca gatatgcgat
     7981 agtccggcat tgttgaaaga ggaggcggcg gttggcggga gctatccgaa tgcgagtata
     8041 aagatggagg agctggtgag ggagatagag gagaatacgg gggatcacaa ggctttagtg
     8101 ttttcgcagt ttgcgacgat gttgcggttg atcgggagtg agctggagag tcgggggata
     8161 ccttatctgt acctggatgg cggggtgccg gcggaggagc gcaagacatt ggtgcggcag
     8221 ttccaggagg aggatgctgc ccgcgtattt cttatcagtc tgaaggcagg cggggtaggg
     8281 ctgacgctga cagcggccga ttatgtctac ctggtggatc cctggtggaa tcccgcggcg
     8341 gagcagcagg ccatcgaccg gagtcaccgg atcgggcagg tgcgcaaggt gtttgcgtac
     8401 cggatgatct gtaaggatac ggtggaggag aagatattgc agctgcagca gaagaagaag
     8461 accctggcgg aggacctggt gggggatgaa tcgggttttg tgaagaagct gacggccgag
     8521 gatgtggcgt atctgttcag ctaaatcatt aaatttgatg atatgaactg gtcgactttc
     8581 cttcggacgc tgttggaggt agcggacaaa tattatatac tcgcggggat cgcattcctg
     8641 gtatattatg ttttgctgcg ccggcgaagc tccgggaaaa agatacagcc gaggtttccc
     8701 ggcgcgaagg attatcaacg cgaggtggtt ttttccacca tttcgatcct catttttgcg
     8761 atgccgcccc tcttgcttgt gcatacggac gcattgaggc cgcatacgac gttttataag
     8821 gacataaggc tgcatagccg gtggtatttt ttcggggcct ttccgctgct gttccttatg
     8881 catgacgcct atttttattg gatgcaccga ttgatccatc acccgcggct cttccgcctg
     8941 ttccacctgg tacatcaccg gagcgtcaac ccttcgccgt gggcggccta tgcttttcat
     9001 ccgctggaag catttctcga atcgctgata tttgtcattt tcctgtttac gatcccgatc
     9061 aacgtatggc atttgttctt ttttttcctg atcagcctgg cgtacaacgt gtacggacat
     9121 ttgggttttg aactataccc gtcaggattc aacaggcact ggttggggaa atgggtgaat
     9181 acctcggtga gtcacaatca gcatcaccgt cattttaagg gtaattacgg gctgtatttt
     9241 ttatt
//
LOCUS       DASYSV010000260         4655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_49026, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000260 DASYSV010000000
VERSION     DASYSV010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49026"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="VGQ51_07980"
     CDS             complement(<1..1654)
                     /locus_tag="VGQ51_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036542.1"
                     /translation="MLKSYWTLAWRTLVRNKFYTLVNVLGLALGICACVAIYSITRYE
                     FSFDTFHPDRDRIYCVDIETPRGPMLAVPGSMPGRMRQGMTGFETVAAYWYYVANVTI
                     PAVRNEKPKRLDDAGSIAVAEPQYFDIFPHRWLSGDASTSLSMPNSVVLTSEKAREYF
                     GDLDPDKVIGRTLYYEDSLPCTVTGVVADWKGNSDFKFDQWISYATIPVSFLQQRMPL
                     IDNGMFSNSSQVMIKLAKAVTPSQIDAQLAGYSKRYFDGHWAMHLRPLSDIHFHTEYG
                     GRARKASLPVLYTLIAVAGFILILAIINFVNLSTAQSTQRAREIGVRKVMGGSRAGIA
                     VQFFTETLLVTIAAVGLAALIIRPVFSLFADILPPGIHFRLDAETGLFLLLVIMVTTI
                     LAGFYPAKVLGNYQPTVTLKGATAVSGGGKAALRKGLIVFQFTISLLFIIASLVIGSQ
                     LRYALHADLGFKTDAIVTLPFARGDKTGKVTVLADKIRQMTGVAQVIQESMPPIGPMH
                     SSFGGGGLKNSSEQPFDVSIKYGNEDFVPFYGMKVVAGRNLLP"
     gene            complement(1703..4261)
                     /locus_tag="VGQ51_07985"
     CDS             complement(1703..4261)
                     /locus_tag="VGQ51_07985"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HEV9036543.1"
                     /translation="MIQSKLYPRANGSCLSPSKVWHAILMVIAIFVACSLHGQAIVVG
                     YSSSTTTAGIRFSEDKPMPLIAVDAADHAVVKLAASLFAADVYRVTGRQPQVDTLGAK
                     ATEMIIAGTIGESGLIDRLIAAGKLADIDKIKGHWEASLWQVVRDPMPGVRRALVIVG
                     SDRRGTAYGFMVLSQKIGVSPWYWWADVPVTHHSVLSLSMSGPAWDEPSVRYRGIFIN
                     DEDWGINEWARQTFEKDFGNGVGPKTYEKVFGLMLRLRLNYIWPAMHEVSKEFGNTPE
                     NVALADRYAIVSGSSHCEPMLYNNVHWDESKKGRWNYSTNRDTIFETWKTTVLDRRDK
                     EAVWTLGIRGIHDRGMETPPAGAMDRIGLLGQVFRDQRELIDKYVTRRYGQPAECFVP
                     YKEVLPVYDAGLKVPDDVTLMWVDDNFGYIRRLSNPEERKRPGGSGLYWHLSYYGAPH
                     SYTWINTTAPALMWEEFRKAWENQARTIWVINVGDIKPMELGIDYFARLSWDPADSAA
                     RRPDAQPVFLRSFIAENFGAALKGPLSALLMDYYRLGTVRKPELMNRAWCLSLPRRRA
                     EGLRAAYEGLLKRETAVYAKIPSAARDAYMELIGFPARILAATGLIFLHDRAAVLGSD
                     PRLQSLEIDRLKSFIGRQLDTYNDTVAHGKWRFMMPGMVTGKNLPAWSSQVAWPWGET
                     RPPDTAKRDIEPDLLMPASSFARELRAAKAKWTVVPGLGQSGSAVALEPAGPDAIWGP
                     ETISAPTLEYRFTGGGRGRKAIVYFMPTFRIYPGMQLRVTVGVDGKPIMTLEVPGSNG
                     KEDENGPNRNEGIRNNCVGAVVELPDLPEGRHTLFIRAVDPGVVIDRIGFIGPR"
     gene            complement(4419..>4655)
                     /locus_tag="VGQ51_07990"
     CDS             complement(4419..>4655)
                     /locus_tag="VGQ51_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV9036544.1"
                     /translation="ARRSAKGKDIRIQGGANTIQQFLNAGLVDECFIHIAPVFLGSGI
                     RLFDGMVKDKYDIQIEEVIPSDLTTHLRYKLTKK"
BASE COUNT         1052 a         1369 c         1165 g         1069 t
ORIGIN      
        1 ttgggagcag gttgcgtccg gccactacct tcattccata aaaagggacg aaatcttcgt
       61 tgccgtattt gatggacacg tcgaagggtt gttcactgct gtttttcagc ccaccgccgc
      121 cgaatgaact atgcatgggg cctatcggcg gcatggactc ctgtatcacc tgtgctacgc
      181 cagtcatctg gcggatctta tccgccaata ctgtcacctt acctgtcttg tccccccgcg
      241 caaacgggag cgtcacgatc gcatccgtct tgaaacccag atccgcgtgc agcgcgtatc
      301 gcagctgtga accaatgacg agcgacgcga tgatgaacag aagcgatatg gtaaactgga
      361 agacgataag acccttgcgt agcgccgctt ttccgccgcc ggacaccgca gtggcccctt
      421 tcagggtcac cgtgggttga taattcccca gcactttggc cggatagaat cccgcaagaa
      481 tggtggtcac catgatcacc aaaagcaaaa acaatcctgt ttcggcatcc aaccggaaat
      541 gaattcccgg cggtaaaata tctgcgaaca ggctgaacac cggtcttatt ataagcgccg
      601 caagacccac tgccgctatc gtcacgagca gggtctcggt aaaaaattgc acggcaatac
      661 ccgccctgct gccgcccatc acttttcgaa cgccgatctc tctcgccctt tgtgtggatt
      721 gcgcggtaga caaattgaca aaattgataa tggcgaggat caggataaag cccgccacgg
      781 cgatgagtgt gtatagcacc ggcaggctgg ccttcctggc ccgaccgcca tattccgtat
      841 gaaaatggat gtctgacaac ggtctcaaat gcatagccca gtgcccgtcg aagtatcgtt
      901 tggaataacc ggccaattgc gcatcgatct gcgaaggggt gaccgccttt gccaatttta
      961 tcatgacctg gctcgaatta ctaaacatcc cattgtcgat cagtggcatc cgctgttgca
     1021 ggaagctgac cgggatcgtg gcataagaga tccactggtc aaacttgaaa tcgctgttgc
     1081 ccttccaatc ggcgacgacc ccggttaccg tgcatggcag cgaatcctca taatagaggg
     1141 tccggccgat gaccttgtcc gggtcgagat cgccaaaata ttccctcgcc ttttccgacg
     1201 taagcaccac cgagtttggc atgctcagcg aagtcgaggc atctccgctc agccaccggt
     1261 gcgggaagat gtcgaaatat tgcggctctg cgacggcaat gctgcctgcg tcgtcgagtc
     1321 ttttcggctt ttcgttccgg accgctggta tcgtcacatt ggcgacataa taccaatagg
     1381 cggctacggt ctcaaagccg gtcattccct gccgcatgcg gcccggcatg gacccgggta
     1441 cagcgagcat agggccgcgg ggtgtctcta tgtcgacgca atagatgcga tcgcggtccg
     1501 gatgaaaggt gtcgaaactg aattcgtacc tggtgatcga ataaatggcc acgcaggcgc
     1561 aaatacccag cgccaggccc aggacattga caagggtgta gaacttgtta cggaccaggg
     1621 tccgccaagc cagcgtccag tagcttttga gcatatattc aatttgctat taagatacgg
     1681 tattttaggg aaggtgcttt cttcaacgtg gcccgataaa cccgatcctg tcgataacca
     1741 cgcccgggtc tacggccctt ataaacagcg tatgcctgcc ttccggcaag tccggcagtt
     1801 ccactaccgc gccgacgcag ttgttccgaa ttccttcatt cctgttcggt ccgttctcat
     1861 cttccttacc attcgaaccc ggcacctcca gtgtcattat gggcttgcca tccactccaa
     1921 cagtcacccg aagttgcata cccggatata tcctgaacgt aggcatgaaa taaacgatcg
     1981 ccttgcggcc ccggccaccg ccggtaaacc gatattccaa cgtcggtgcg gagatagttt
     2041 caggtcccca tatggcatcg ggtcccgccg gttccagggc tactgcactt ccgctttgac
     2101 ccagcccggg taccaccgtc catttcgctt tcgctgcccg caattcacgg gcaaatgaag
     2161 acgccggcat caaaagatcc ggttcgatat cgcgcttagc cgtatccggc gggcgggtct
     2221 caccccaagg ccaggcaacc tggctgctcc aggccggtaa gttcttgccg gtaaccatac
     2281 ccggcatcat gaaacgccac ttcccatgcg cgaccgtgtc attgtaggtg tcgagctgcc
     2341 ggccgataaa tgacttcaga cgatcaattt ccagcgattg aagacgggga tcgctcccca
     2401 ggacagcggc ccgatcgtgg agaaagatca gtcccgtagc ggcaagtatt ctggctggga
     2461 aaccgatcag ttccatatac gcgtcccgcg ccgccgacgg tattttcgca taaactgcgg
     2521 tctcgcgttt caacagtcct tcataggcag cccgtaaccc ctctgcgcgc cggcgcggta
     2581 gcgacaggca ccaggcccgg ttcatcagtt caggttttct taccgtgccc agtcgataat
     2641 agtccatcag caacgcagac agcggcccct tcaatgcggc tccaaaattc tccgcaataa
     2701 atgatctcag gaataccggt tgcgcatcgg gccgccgggc cgccgaatcc gccggatccc
     2761 atgacaacct tgcaaaatag tcgatcccca gctccatcgg tttgatatcg cccacattga
     2821 tcacccaaat ggtacgtgcc tggttttccc atgctttccg gaactcttcc cacatcagcg
     2881 caggcgcagt tgtattgatc cacgtataag aatggggggc gccataatag gacaagtgcc
     2941 aatataaccc gctgccgccc ggccgttttc tttcctccgg gttggaaagt ctcctgatat
     3001 aaccgaaatt atcatcaacc cacatcaacg taacgtcatc cggcactttt agtcccgcat
     3061 catataccgg caacacttcc ttgtagggta caaagcactc tgccggctgc ccgtaccgcc
     3121 tggtaacgta cttgtcgatc aattcccgct ggtcccggaa cacctgaccg agcaacccga
     3181 tacgatccat agcgcccgcc ggcggcgtct ccatgccacg atcgtgaatg cccctgatgc
     3241 ccagcgtcca tacggcctcc ttgtcccgcc ggtcgagaac cgtagtcttc caggtctcga
     3301 atatcgtatc cctgttggtg ctgtaattcc agcgtccctt tttactttca tcccaatgaa
     3361 cattattata cagcatcggc tcacagtgcg atgatcccga aacgatggca taccggtcgg
     3421 caagcgccac attttcaggc gtgtttccga actctttgct tacctcatgc atggccggcc
     3481 atatataatt caatctgagg cgaagcatta acccgaacac cttttcatag gtcttcgggc
     3541 cgacgccatt gccaaagtct ttttcaaatg tctgcctggc ccactcattg atcccccaat
     3601 cctcgtcatt gatgaatatg ccccgatacc tgactgaagg ttcatcccat gcaggtcccg
     3661 acatagacaa ggacaggacg ctgtggtgag tgacagggac atcggcccac cagtaccagg
     3721 gggagacccc gatcttttgc gacagcacca taaatccata tgccgtgccg cgtctatcgc
     3781 ttccaacgat gaccaaagcc ctccggacac ccggcatcgg atcccttacc acctgccata
     3841 aggaggcctc ccaatgccct ttgatcttat cgatatcggc cagcttgccg gcagctatca
     3901 aacgatcgat caaaccgctc tcgccgatgg tccctgcaat gatcatttcc gtcgccttcg
     3961 cgccgagggt atcgacctgc ggctgccggc ctgtgacacg atatacatca gcggcaaaga
     4021 gggatgcggc aagtttcacc acggcatgat cagcagcgtc aacggcgatc agcggcatag
     4081 gcttgtcttc ggagaaacgg atcccggcgg tggttgtcga agaagaatat ccgacaacaa
     4141 tggcctgacc atgtaaagag caggccacga agatggcgat gaccatcagg atggcatgcc
     4201 agactttaga agggcttaaa caacttccat tggccctcgg atataatttc gactgtatca
     4261 tcggtcactt tgatggcggt ttgatcatcg atcgcagcgt tggcctcgga aaccggattg
     4321 cccaggaggt caacgagtgc gttgtggatg cttgtgttgc tgatgcccgc ggacgtaaga
     4381 agaagcttca taattgaagg gtaatggcgg gagtgttgtt atttttttgt caacttgtat
     4441 ctcaggtgtg ttgtcaggtc agacgggatc acttcttcga tttgaatatc atatttgtct
     4501 ttgaccatgc cgtcaaacag ccggatgcca cttcctaaaa aaacaggcgc aatatgaatg
     4561 aagcattcgt ctacgaggcc tgcattgaga aattgttgaa tggtgttggc gccgccctgt
     4621 atccttatgt cctttccttt ggctgatctt cttgc
//
LOCUS       DASYSV010000261         8144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1969092, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000261 DASYSV010000000
VERSION     DASYSV010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969092"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /gene="paaD"
                     /locus_tag="VGQ51_07995"
     CDS             complement(<1..431)
                     /gene="paaD"
                     /locus_tag="VGQ51_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEV9036545.1"
                     /translation="MEEIKTIWSLLETVEDPEVPVLSVIDLGIIREVSLENGQPVIVI
                     TPTYSGCPAMDMIRVRIRMAMMEHGFSDVQIRTVLSPAWTTDWMSENGKEKLKAFGIA
                     PPMPMPIVCHPGMFHQDEAIPCPRCNSYRTTRISEFGSTAC"
     gene            complement(436..1212)
                     /gene="paaC"
                     /locus_tag="VGQ51_08000"
     CDS             complement(436..1212)
                     /gene="paaC"
                     /locus_tag="VGQ51_08000"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEV9036546.1"
                     /translation="MSSPAIIEYTLHLADNALVLGHRNSEWTGHGPILEQDIALSNIA
                     LDLIGQARNLYQSAAGVMGTDGREERATTTEDDLAYMRDAWDFRNCLLVEHPNGDWGM
                     TMLRQFFFSAWQYYFYGELRHSRDNMLAAIAVKSLKEVTYHLRWSGEWVIRLGDGTDE
                     SHSRMVKAMEHLWQFTGELFEPAAYEKMLVAEGIAVDLAVIRPMWDQRVNEVLSAAQL
                     TYGSSGWMQTGGKQGKHTEHLGYLLAEMQFLQRAYPGCEW"
     gene            complement(1214..1516)
                     /gene="paaB"
                     /locus_tag="VGQ51_08005"
     CDS             complement(1214..1516)
                     /gene="paaB"
                     /locus_tag="VGQ51_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009882064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEV9036547.1"
                     /translation="MEKETGSAGTWPLWEIFIRSKQGLDHKHVGSLHAADAAMAIENA
                     RDAYTRRMEGVSIWVVESKYIHASNPDEAASLYDPANDKVYRHPTFYDLPDELKHM"
     gene            complement(1518..2465)
                     /gene="paaA"
                     /locus_tag="VGQ51_08010"
     CDS             complement(1518..2465)
                     /gene="paaA"
                     /locus_tag="VGQ51_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEV9036548.1"
                     /translation="MSSSVNEHGFQERIDQDIRIEPKDWMPDAYRKTLIRQISQHAHS
                     EIVGMLPEANWITRAPSLRRKATLIAKVQDEAGHGLYLYAAAETLGISRDEMIEQLHS
                     GKAKYSSIFNYPTLTWADMGAIGWLVDGAAIMNQVPLCRASYGPYARAMVRICKEESF
                     HQRQGFEILLVLSRGSESQRRMCQDAIDRWWWPSVMMFGPNDDASPHTAQSMRWKIKR
                     FTNDELRQRFIDVCAEQVKLLGMTLPDENLVWNEERGHNDFGPIDWEEFRNVINGNGP
                     CNRQRLEARVQAHVDGAWVREAALAHARKRSNLNKEEAA"
     gene            2563..3168
                     /locus_tag="VGQ51_08015"
     CDS             2563..3168
                     /locus_tag="VGQ51_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEV9036549.1"
                     /translation="MPLLTRCRRILFSPIAEWQRIGKECSPVRALLAGYVIPMALIPA
                     VASFIGYGFIGVNGLRFRVRGLYWGSAMAIDSFITSLVVYWLGTYIVDWLGIGFGSKR
                     NAKRAAQLVAYSYTPAWIAGIFFLWPSLHGLIALGLYGVYLFYLGIPVLKPGQRDQRI
                     SYTIISAILLIVVRFLIGLLIVNIIFSFWGDPYLPFSPALF"
     gene            complement(3160..4233)
                     /gene="paaE"
                     /locus_tag="VGQ51_08020"
     CDS             complement(3160..4233)
                     /gene="paaE"
                     /locus_tag="VGQ51_08020"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEV9036550.1"
                     /translation="MKIEDVRRETADCVSIAFHIPPELREAFRYSQGQNITVRTFVAG
                     MEVRRSYSICSSPLDNELRIAVKKVPGGQFSTFANEVLQPGHELEVLTPSGKFYTELR
                     PENQKRYLAFAAGSGITPVISLIKTTLAVEPQSHFTLVYGNRHRPSIIFRDQLEALKN
                     RYMQRLALHHILSREEMDIPLYQGRIDADKCRELCDGVIDLSSTDEVYLCGPEEMIFT
                     VRDWLEQQGVEKKKIHFELFHPLDKTGIATRERQTSPENGAAATEKTSQVTVRLDGIN
                     HVFNLPFEGASILDAALYEGVDLPFACKGGVCCTCRARLTEGQVEMDLNYALEADELA
                     AGFILTCQSHPRSPKVTVDFDSK"
     gene            complement(4255..4845)
                     /locus_tag="VGQ51_08025"
     CDS             complement(4255..4845)
                     /locus_tag="VGQ51_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV9036551.1"
                     /translation="MAKIQSKGNSTKKEVIIEKASRLFREKGFGAASMRDLADHVGVE
                     AASLYNHIQSKSEILQTICFRVANAFMSNLESVETSAQPILKKMESIIRLHIRMMLDQ
                     YEYVYIADHEWRHLPEPYLSNFLNQRRNYRKRLGDIIEDGITRGEIKTIEPYTAVLVI
                     LSAISGIDSWQRSRKSTTAEALEANMVKYLIEGLKK"
     gene            5032..5679
                     /locus_tag="VGQ51_08030"
     CDS             5032..5679
                     /locus_tag="VGQ51_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036552.1"
                     /translation="MTPFFSRLGRFLLLALVPIALVAFLVWWLLQLTTVNDILAAVII
                     HILAGTLVAFLFLYLVLVFMRPKLMISKYICKRGDMYLFKIVNTSRYHAFDVKIEMFK
                     MVPVSHTGGRSNVSIHHIKMHTSEWTSINRVRRKFSDKDPFCLFAQVIFTEVDIETPL
                     FENGTFLEMRLTARHGLTGFADRFVQQFVNESVLKVGHKFCFGVDLGTMPENQAF"
     gene            complement(5872..6840)
                     /locus_tag="VGQ51_08035"
     CDS             complement(5872..6840)
                     /locus_tag="VGQ51_08035"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEV9036553.1"
                     /translation="MNFKTLTELYDYFKTEEVCIAYLEQQRWGGTPACPFCGVINPYR
                     TNRGFKCREKACGKKFTVKVGTIYENSKIPLRTWFAAGYLCLSSKKGVSSLQISRQLG
                     ITQKTAWFVLHRIREMLKDKAPQMLSGTAQIDECYVGGLEKNKHSNKRAKQGRGKSGW
                     ANDTKTPVFGIAETGGKVIVEVSEWVTKREARQLIEKHVQKGSNMVTDGLSMYAFLGE
                     DDRFKHYVVDHKAGQYVNGGFHTNGIENFWSLLKRGIIGIFHQISPQHLQRYCDEFAA
                     RYNTRKIKDHERFDLCQQRVNGRLTYRQLIGKSKGTRGDAFSQMME"
     gene            complement(7328..7861)
                     /locus_tag="VGQ51_08040"
     CDS             complement(7328..7861)
                     /locus_tag="VGQ51_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036554.1"
                     /translation="MSDKITLDIDIADAPEYIDLIQKMRLAVDEEISKRQRELSALVT
                     KSVQLGFMLEKLGSSPSGIPIVVDSNIIIGGNGNRQVAPVKVINGYNHKWTVWEKIQY
                     VLRKQIEPISKIDIIKILEGLEPGLAELRGAKYRTFSVSISACLSTKSANGLLARARA
                     TNKDDYKYYLPNKEATE"
BASE COUNT         1818 a         2134 c         1924 g         2268 t
ORIGIN      
        1 ttacaggccg tactgccgaa ctcgctgatc cgtgtggtgc ggtaagaatt gcatcgggga
       61 cagggtatgg cttcatcctg gtggaacata cccggatggc agacgatcgg cataggcatg
      121 ggcggcgcta tcccgaacgc cttcagcttt tccttgccat tttcgctcat ccagtccgtg
      181 gtccatgccg gcgaaagcac cgttcggatc tgcacgtcgg agaaaccgtg ttccatcatc
      241 gccatcctga tcctgacccg gatcatatcc atcgccgggc agccactata ggtaggcgtt
      301 attacgataa ccggctggcc gttttcgaga cttacctcgc ggatgatgcc cagatcgatc
      361 accgacagga ccggcacctc ggggtcttca accgtttcta ataacgacca tatggttttg
      421 atctcctcca tcgtactacc attcacaccc cggataggcc ctttgcagga attgcatctc
      481 ggccagcagg tagcccaaat gttcggtatg ttttccctgc ttgccgccgg tctgcatcca
      541 cccgctgctg ccatatgtca gctgcgccgc gctcaatact tcattcaccc gctgatccca
      601 catcggccgg atgaccgcaa gatccacggc tataccttcg gcgaccagca tcttttcgta
      661 tgccgcgggc tcgaaaagct caccggtgaa ttgccacagg tgttccatcg cttttaccat
      721 ccgggaatga ctttcatcgg tgccgtcgcc gagacggatc acccattcgc cgctccatcg
      781 cagatgatag gtcacttctt taagcgattt caccgcgatg gcggccagca tgttatcccg
      841 gctatgtcgc agctcgccat aaaagtaata ttgccaggcg ctgaaaaaaa actgtctcaa
      901 catcgtcatc ccccaatcgc cattggggtg ttcgacgagc agacaattcc tgaaatccca
      961 ggcgtcacgc atataagcca gatcatcttc cgtagtcgtc gcgcgttctt ccctcccgtc
     1021 ggtacccatg accccggccg ccgactgata aagattcctc gcctgcccga tcaggtccag
     1081 ggcaatattc gacagggcga tatcctgctc gaggataggg ccatggccgg tccattcgct
     1141 gttgcggtgg cccaggacaa gcgcgttgtc cgccaggtgc agggtatatt cgatgatggc
     1201 tggagacgac atcttacata tgctttaatt catccggcaa atcgtagaag gtcgggtggc
     1261 gataaacctt gtcattggcg ggatcgtaca gcgatgcagc ttcatcggga ttcgaagcat
     1321 ggatatattt actttccacc acccagatgc tgacgccttc cattcgacgg gtataggcat
     1381 ctctggcgtt ctctatagcc attgccgcat ccgcggcatg cagactgccg acatgtttgt
     1441 gatcgagacc ctgtttgctg cggataaaga tctcccacag cggccatgta cccgcgctac
     1501 ccgtttcttt ttccatatta tgcggcttcc tccttattga gatttgaacg ttttcgtgca
     1561 tgcgccaacg ccgcttcccg cacccaggct ccatccacat gcgcctgtac ccttgcctcc
     1621 agccgctgcc ggttgcaggg gccgttaccg ttgatgacat tcctgaactc ttcccagtcg
     1681 atcggcccga aatcattgtg cccgcgctcc tcattccata ccaggttttc atccggcagg
     1741 gtcattccca gcaacttgac ctgttccgcg cagacatcga tgaaacgctg ccgtaattcg
     1801 tcattggtga atcgtttgat cttccatcgc atactttgcg ccgtgtgcgg tgaagcatcg
     1861 tcattcggtc cgaacatcat gaccgagggc caccaccacc gatcgatcgc atcctgacac
     1921 atccttcgtt gcgactcgct tccgcggctc agcacaagca gtatctcgaa accctggcgt
     1981 tgatgaaagc tttcctcctt acaaatcctt accatcgccc tggcataagg accataggag
     2041 gccctgcaca ggggcacctg gttcatgatc gcagcgccgt cgaccagcca gccgatggct
     2101 cccatatctg cccaggtcag cgtgggatag ttgaagatag aagaatactt tgccttgccg
     2161 ctatgcagct gttcgatcat ctcgtcccgg cttataccca gcgtctcggc cgccgcatag
     2221 agatacagcc catgtcccgc ctcatcctgc acctttgcga tcagcgtagc cttcctcctg
     2281 agactgggcg cccgcgtgat ccagttggcc tccggcagca tacccacgat ctcgctgtgc
     2341 gcatgttgcg atatctgcct tatcagggtc ttccggtaag cgtcgggcat ccaatccttc
     2401 ggctcgatcc ggatatcctg atcgatcctt tcctggaaac catgttcatt gacggaagac
     2461 gacatgcaat ttttgtttat ccgcaaatat acttcttttt cgaaagctaa caactgttag
     2521 tatccgggca ggcgtattta agggattgct tatctttcgg gtatgcccct tctgacccgt
     2581 tgtaggcgga tactcttttc tcccatcgct gaatggcaaa gaataggaaa ggagtgcagc
     2641 ccggtgagag cgcttctcgc cggctacgtg atccccatgg cccttatacc cgctgtcgcc
     2701 agcttcatcg gctatggctt tatcggggta aatggtcttc ggttccgggt acgcggtctg
     2761 tactgggggt ccgccatggc gatagatagc tttatcacct cactggtcgt atattggctg
     2821 gggacctata tcgtcgattg gctgggcatc ggattcggat cgaagagaaa tgcgaaacgg
     2881 gctgcgcagc tcgtggccta ttcttacacg cccgcctgga ttgcgggcat cttttttcta
     2941 tggccatcat tgcacggatt gatagcgttg ggactctatg gcgtgtatct tttttacctg
     3001 ggaatacctg tcctgaaacc cggtcaacgc gatcagcgaa tcagctatac gatcatcagc
     3061 gccatcctcc tcatcgtcgt ccgcttcctg atcggtttgt tgatcgtcaa tatcatcttc
     3121 tctttctggg gcgaccctta tcttcctttc agcccggctt tattttgaat cgaaatcgac
     3181 cgtcactttc ggggaccgcg gatgcgattg gcaggtgagg atgaatccgg ccgccagctc
     3241 gtcggcttcc agtgcatagt tcagatccat ttccacctgt ccctcggtca gtcgtgcgcg
     3301 gcaggtacag cagacccctc ctttgcacgc gaagggcaga tcgactccct cataaagggc
     3361 agcatccagg atagaggctc cttcgaaagg aagattgaag acatgattga tgccgtcgag
     3421 acgtaccgtc acctggctcg tcttttctgt cgccgccgca ccgttttccg gcgacgtctg
     3481 ccgctcccgg gtcgcgattc ccgttttatc caaaggatga aagagttcga aatgtatctt
     3541 ctttttctcc actccctgtt gttccagcca gtctctgacg gtaaagatca tctcctccgg
     3601 tccgcacaga tagacctcgt ccgtcgacga aagatcgatc accccgtcgc acagctcccg
     3661 acatttatcc gcatcgatac gcccctggta caaggggata tccatttcct cacggctgag
     3721 tatgtggtgc aaagccagac gctgcatata gcgattcttc agggcctcca gctgatctct
     3781 gaaaatgatg gaagggcggt gacgattgcc gtataccagg gtgaaatggc tttgcggttc
     3841 tacggcaagc gtggtcttga tcagcgagat caccggcgtg ataccgctgc ccgcggcaaa
     3901 ggccagatag cgcttttggt tttccggccg gagttcggtg tagaattttc cggaaggcgt
     3961 caacacttcc agctcgtggc cgggctgcag cacttcgttg gcaaaggtgg agaattggcc
     4021 gccgggtact tttttcacgg cgatgcgcag ctcattatcg agcgggctgc tgcatatgga
     4081 atacgaacgc cgcacttcca ttccggcgac aaaggtgcgg acggtgatat tttgtccctg
     4141 ggaataacga aaggcttctc tgagctccgg ggggatgtgg aaggcgatgg atacgcaatc
     4201 ggccgtttcg cggcggacat cctcaatttt caatcgttgg aaatgggcca cgtattattt
     4261 ttttaacccc tcgatcaggt attttaccat attcgcctcg agtgcttcgg ctgtagtgct
     4321 tttgcgcgat cgctgccagg aatcgatgcc gctgatggca gataggatga ctaagacggc
     4381 ggtatagggt tctattgttt tgatctcgcc cctggtgata ccgtcttcga tgatatcccc
     4441 caggcgtttg cggtagttgc gccgctgatt gaggaaattc gagagatagg gctccggaag
     4501 atgccgccat tcatgatcgg cgatatagac atactcatat tgatccagca tcatccggat
     4561 atggagccgg atgatcgatt ccattttctt gaggatgggc tgggcgctgg tctccaccga
     4621 ttccaaattg gacatgaagg cattggccac gcggaaacat atcgtctgca gtatctccga
     4681 tttggattgg atatggttgt aaaggctggc agcctccacc cccacatgat ccgccagatc
     4741 gcgcatcgac gcagcgccga atcccttctc cctgaagagg cgggaggctt tctctatgat
     4801 cacctctttt ttggtgctgt tgcctttgct ctggatttta gccatagatt ttattggccg
     4861 cgccggccca gcgacaaata tagtaataac taacgagtgt tagccgggaa ttgtggcaac
     4921 tatataccag ttgggacaga ggaatttaag attggaaagg gttaaatcac tcttttgcta
     4981 gataggtttt ttccttatct tccgcggcgt ttaacttaat tatgtagaaa tatgacccca
     5041 tttttttcaa gattaggcag gttcctattg ttggcattgg ttcctattgc gttggttgcg
     5101 tttttagtct ggtggttgct tcaacttacc accgtcaacg acattctagc ggcagttata
     5161 attcatatac ttgccggaac tttggtcgca tttttattct tatatctcgt cttggttttc
     5221 atgcggccca agctgatgat atccaaatat atatgcaagc gtggcgatat gtatttattt
     5281 aagattgtaa atacctctcg ttatcatgcc tttgatgtaa aaattgaaat gtttaaaatg
     5341 gtaccggtta gtcatactgg cggtcgttcg aacgtctcta ttcatcatat aaaaatgcac
     5401 acctctgaat ggacaagtat taatcgcgta cgaaggaagt ttagcgataa agacccattt
     5461 tgcctatttg cgcaagtcat atttacggaa gtagatattg aaaccccatt atttgaaaat
     5521 ggcacttttc tggaaatgcg cctgacagct cgtcatggtt tgaccggatt tgctgacaga
     5581 tttgtgcaac aattcgttaa tgaaagcgtt cttaaggtgg ggcataagtt ctgctttggg
     5641 gtagacttgg gtacaatgcc ggaaaatcag gcattttgat aatcggtaaa tgttttgtat
     5701 attatattgt ttaattttat aaaaatttca gaaaatgaaa cctcgtctta gtcgtgatgg
     5761 tgaagcgaca aaaagctaat taaccgatcc aaaaatttta aacagcaaag gttgccatat
     5821 ggcagccttt gctgttatat aggtccgcaa tctcatacga aatactattt attattccat
     5881 catttgtgag aaagcatctc ccctggttcc cttgcttttg ccaataagct gcctataggt
     5941 taagcggcca tttacccgct gctggcagag atcgaagcgt tcatgatcct ttatcttccg
     6001 ggtattgtac cgggccgcaa actcgtcgca atagcgttgg agatgctgcg ggcttatttg
     6061 gtggaatatg ccgatgatcc cccgtttcag gagggaccag aagttctcaa taccattggt
     6121 gtgaaaacct ccgttcacat attgacctgc tttgtggtca actacataat gtttgaaccg
     6181 gtcatcttcc cccaaaaaag catacataga caagccgtcc gttaccatat tggaaccttt
     6241 ctgcacatgc ttttcgatta actgccgtgc ttctcttttc gtgacccact ccgaaacctc
     6301 aacgataacc ttcccgcccg tctccgctat gccgaaaact ggcgtcttag tatcattcgc
     6361 ccaaccgctt tttccccttc cttgttttgc cctcttgtta gagtgcttat tcttctccaa
     6421 acctcccacg taacattcat ctatctgcgc cgtaccactc agcatttgcg gagctttatc
     6481 tttaagcatt tcacgtatgc gatgtaggac aaaccacgcg gttttctgtg tgatgcctaa
     6541 ctgacggctg atctgcaaac tgctgacacc cttcttcgag gaaagacaga ggtagccggc
     6601 ggcaaaccat gtgcgaaggg gaatcttgct gttctcgtag atcgttccaa ccttaaccgt
     6661 gaacttcttt ccacaggctt tctcccggca tttgaaaccg cgattcgttc tataaggatt
     6721 gatcactccg cagaacgggc atgcaggagt acccccccaa cgctgttgct ccaggtatgc
     6781 aatgcaaact tcttcagtct tgaagtagtc gtacagttcg gtgagagtct tgaagttcat
     6841 aaattaggta gttgatggga caaatgtaac acaagtccca gtatatacct aattttatcc
     6901 aaactatttt ttacttttgc aagacaggaa aggcccccgg aaggggggcc aatggctgcg
     6961 actccatatc ctgattgggg tgtctggttg ggggctgcgg ggcggttcta tcgccgtccc
     7021 ggcctataat gtgggtgagg atagactcca acccttaccc gcttcctcaa tatttttaca
     7081 tcccaaatgt gctatatcaa atgatatagc aattgctata tccaaggata tatcctatat
     7141 ttgtagtaat aacagggcgc gtccgctgtt ggaaggcggc cgcttctgag gtttattcga
     7201 tgtcttggta ttagttggag ctactacctc ggattctcac cgaacatccc ccatccctgt
     7261 taagtatgtt tgacatcttg ctttacgtaa ttggccggat ttgtctagta aatggatccg
     7321 gcctttttca ttctgtcgcc tccttatttg gtaaatagta tttataatca tccttgttgg
     7381 ttgccctagc ccttgcaagc aaaccgttcg cagacttagt cgataagcaa gcggaaattg
     7441 ataccgaaaa cgtcctgtac tttgcgccgc gcaattcggc taaccctggc tccaatcctt
     7501 ccaaaatttt aataatgtcg atcttgctga taggctcaat ctgtttgcgc aggacgtatt
     7561 gtattttttc ccatactgtc cacttgtgat tgtacccgtt gattaccttt accggtgcca
     7621 cttgccggtt gccgttaccg ccaataataa tattactgtc tacaacaata ggaatgccac
     7681 taggcgagct acccagcttt tcaagcatga acccaagttg aacagactta gtcaccaatg
     7741 cgcttaattc cctttgacgt ttggatattt cctcgtcaac ggcaagacgc atcttttgaa
     7801 ttaggtcgat gtattccggc gcatctgcaa tgtcgatatc taatgtgatc ttgtctgaca
     7861 ttaatcaatg attacgggcg aatatacgaa caatatttat gcaaccacgg catgcatata
     7921 ttattatgta tcacttattt acaccaattt ttttgcacat ccgtggaaag aaagttcttg
     7981 ggaattacca aaatttttcg tatgagcgct aatcgtaata aatggttcgc gcaatcaaga
     8041 aatatctcgt cgttgtcgta taggcatggc agatataagc ataatcattt aagttttcaa
     8101 acagtatgta actgataagt tttattatct ttgaagtatg gcaa
//
LOCUS       DASYSV010000262         3785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2224388, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000262 DASYSV010000000
VERSION     DASYSV010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224388"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VGQ51_08045"
     CDS             complement(<1..658)
                     /locus_tag="VGQ51_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV9036555.1"
                     /translation="MMADLKAIATQIRRDIVRMVHGCQSGHPGGSLGCTEYFTALYFK
                     VMKHDPNFHMDGKNEDLFFLSNGHISPVFYSALARSGYFDIKELATFRKLDSRLQGHP
                     TTHEHLPGVRVASGSLGQGMSVAIGAALSKKLNNDKNIVYSLHGDGELDEGQNWEAIM
                     FASAKKVDNLIATVDWNGQQIDGTTKAVLDTGDLHVKFNAFGWTTLEMNGNDLDEVIA
                     T"
     gene            747..1010
                     /locus_tag="VGQ51_08050"
     CDS             747..1010
                     /locus_tag="VGQ51_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036556.1"
                     /translation="MAGSEWRGREWRGCGGRGVNGRDAEGGEVNGADADGRDVNDRNA
                     EGGDVNGGDADDRDVDSRDAEGGDADDRDVDSRDANIPSIGVW"
     gene            1290..2345
                     /locus_tag="VGQ51_08055"
     CDS             1290..2345
                     /locus_tag="VGQ51_08055"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEV9036557.1"
                     /translation="MKKLFDMPDARKVHFTPITPLGGLGIYAGFIFGSLLTLHFTQYA
                     DLQYFIAASFVIFFLGLKDDILVISPVKKFIGQVLAAFIIIYYGNIQIRSMHGFFGLY
                     QLPEMFSLLLTYFAVIVVINSFNLIDGIDGLAGTLGLLSSVIFGIYFLYIGMSPYAVM
                     AFALAGSLLAFLIFNFQPAKIFMGDTGSLLIGTINAILVIKFINVANTADVAFPIPAS
                     PAVGFTILMVPLLDTLRVFGIRIFHRRSPFSPDRNHIHHLLLDRGFSHRAITLTLVSV
                     NLVMIALVFFCRGLNCTYLILGVFCVFFAIIAAIYYLLPAPRLFVAKTAQEENAQLTK
                     ASHLVTLKKDSILEQNN"
     gene            2618..3181
                     /gene="frr"
                     /locus_tag="VGQ51_08060"
     CDS             2618..3181
                     /gene="frr"
                     /locus_tag="VGQ51_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV9036558.1"
                     /translation="MSDDLELILEVARDSMKKALNHLESELVKIRAGKASPSMLEGLT
                     VDYYGSPTVLNQVANISAMDARTLTIQPWEKNMLQPIERAIINANLGVTPQNDGNIIR
                     LFMPPLTEERRREFVKRAGGEGEQSRVAIRNIRRDGIEQIKKLQKDGLSEDEAKDAEK
                     EIQDITDKHISLVEKHLAAKEKEIMAV"
     gene            3357..>3785
                     /locus_tag="VGQ51_08065"
     CDS             3357..>3785
                     /locus_tag="VGQ51_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HEV9036559.1"
                     /translation="MGKIKLGDIDTRAPKGLDKQETKDKLVKILEELDELQNLLYAES
                     KHCILVVIQGMDASGKDGAVRNVFGKLNPQGVLVHSFKIPTAEELAHDFLWRIHAHAP
                     KKGFIQIFNRSHYEDILITRVHNRIDDETARLRMKAINHFE"
BASE COUNT          876 a          900 c         1039 g          970 t
ORIGIN      
        1 gggtcgcgat tacttcgtcg agatcattcc cgttcatttc gagcgtcgtc cagccgaaag
       61 cgttgaattt cacgtggaga tcgccggtat cgagaacggc ttttgtggtc ccgtcgatct
      121 gttgaccgtt ccaatccacc gtagcgatga ggttatcgac tttcttggcg gaggcgaaca
      181 tgatcgcttc ccagttttgc ccttcatcga gctcgccgtc gccatgcagg gaatacacaa
      241 tatttttatc gttattgagt tttttactca aggccgcgcc gatggcgacg ctcataccct
      301 gtcccagcga accgctggcg acccgtaccc cgggtaagtg ttcatgtgta gtgggatgac
      361 cctgaagacg tgagtcgagt tttcggaatg tggccagttc tttgatgtca aaataaccgg
      421 atcttgcgag cgcggaatag aatacgggag aaatatgacc gttagacaag aaaaaaaggt
      481 cttcgttctt gccatccata tggaaattgg ggtcatgttt catgaccttg aaatagagtg
      541 cggtaaaata ttcggtgcag cccagggaac cacccggatg tccgctttgg cacccatgga
      601 ccattctgac gatatccctt ctgatctgtg tagcaattgc ttttaagtct gccatcatga
      661 ttgattttgg attgattttg tgattgccgg cggcaaatct aatggttttt tcgggactgt
      721 gcgtttaggc gccggcgaag gccctgatgg ctgggagtga atggcgggga cgtgaatggc
      781 ggggatgcgg agggcgggga gtgaatggca gggacgcgga gggcggggaa gtgaatggtg
      841 cggatgcgga tggaagggac gtgaatgaca ggaacgcgga gggcggggac gtgaatggcg
      901 gggatgcgga tgacagggac gtggatagca gggacgcgga gggcggggat gcggatgaca
      961 gggacgtgga tagcagggac gcgaatatcc cctcaatagg ggtttggtga ccttaaactt
     1021 ttgtattatt ttgcggtctg atctctctga ttagccaaaa ttcaaacggg ctttacccgg
     1081 tacaatttgt ttcctgaact gaccctcgag catgcggaac aacgggccca atctttgtta
     1141 agagcataac cgtagtagtg gcattatgaa agaattccta tttttgttca cgaatcaaaa
     1201 tcgtaccgat gtttgatgtc ttactttcga tagctatttc ttttactgta acgtttttag
     1261 caattccggc cattatcaat gtggctgaaa tgaagaagct attcgatatg ccggatgccc
     1321 ggaaggtgca tttcacgcct attacgccgc ttggcgggct gggtatctat gcaggattca
     1381 ttttcggcag ccttctcacc ctccacttta ctcaatacgc tgatcttcaa tattttatag
     1441 cagcatcctt cgtcatcttt ttcctgggtc tcaaggatga tatcctggta atctcgcctg
     1501 taaagaaatt tatcggtcag gtattggcgg cattcatcat catttactac gggaatattc
     1561 agatccggag catgcatggc tttttcggac tctatcaact acccgagatg tttagcctgc
     1621 tgctgaccta ttttgccgtt atcgtggtca tcaactcgtt caatctgatc gacggtatcg
     1681 acggtcttgc cggaacgctc ggtcttttgt cttccgttat ttttggcatt tatttcctgt
     1741 atatcggaat gtctccttat gccgtcatgg cgtttgcgct tgccggtagt ctgctggcct
     1801 tcctgatctt caattttcag cctgccaaga tattcatggg cgataccggt tcgttgctga
     1861 tcggaaccat caacgcgatt ttggtgatca aattcatcaa tgtggcaaat acggccgatg
     1921 tggcattccc gatacccgcc tcgcccgcgg tgggcttcac gatcctgatg gtaccgttat
     1981 tggataccct cagagtgttc ggaatacgca tcttccatcg tcgcagcccc ttcagcccgg
     2041 acaggaacca tatccatcac ttgttgctgg accgcggttt ttcgcatagg gccattacgc
     2101 tgacgctggt atccgttaat ttggtgatga tcgcgctggt attcttctgc cggggtctga
     2161 attgcaccta tctgatcctg ggcgtattct gcgtcttctt cgctataatt gcggccatat
     2221 attatcttct ccccgccccg cgtctcttcg ttgccaagac ggcccaggaa gagaatgctc
     2281 agcttacgaa ggcttctcat ctcgtgacat tgaaaaagga ttccattctg gagcagaaca
     2341 attaattgga acttcggccg atggtgaact tcgctaccgc tcagtcgtgg ctagtcagga
     2401 attgattgga acttcggccc gcttcaccgg agcgagacct tcgtcggagc atcgtccctt
     2461 gctcctcctc ggtcgggccc ttcactttgc tcgtgcccgt tcgcgggctt cgcgcgtggc
     2521 cgacgatgcc tacggctcgt cagaacttac cccaattggt tttgtgtatt tttttgattt
     2581 gacgtaggtt tgccacaacg tacgaaagca taaatctatg tcagacgatc tggaactgat
     2641 cctggaggta gcccgggata gcatgaaaaa agctcttaat catcttgaaa gtgaactggt
     2701 taagatacgg gccggaaagg ccagcccgtc gatgctggaa ggactaacgg tcgactacta
     2761 tggcagtcct acggttctca accaggtggc caatatttca gcgatggatg cccgcacttt
     2821 gaccatccag ccctgggaga agaatatgct tcagccgata gaacgcgcga tcatcaatgc
     2881 caatttgggt gtgactcccc agaatgacgg aaatatcatc cggcttttta tgcctccgct
     2941 gacggaggaa agaaggcggg aattcgtcaa gagagccggc ggagaaggcg aacaatcccg
     3001 tgttgctatc cgtaatatcc gcagggacgg tatcgagcag ataaagaagc tgcagaagga
     3061 cggactcagt gaagatgagg ccaaagatgc cgaaaaagag atccaggaca tcaccgataa
     3121 acatatctct ctcgtggaaa agcatctggc ggccaaagag aaagagatca tggcggtata
     3181 accgggacac ccctacgccc cccttgtcgc gaatatctgt atcatgaacc ccgggcggga
     3241 gtccgggtag aaagcgtttg agtcatgaac cccgggtggg agtccgggga gaaagcgttt
     3301 gagtcatgaa ccccgggtgg gagggagtcc ggggagagca ttttaatcgg agcatcatgg
     3361 gcaaaataaa attgggggat atcgacacgc gcgcgccgaa aggattggac aagcaggaga
     3421 caaaagacaa attggtgaag atcctggagg aactggacga gctgcagaac ctgttgtatg
     3481 cggagtccaa acattgcata ttggtcgtca tccagggtat ggatgccagt gggaaagacg
     3541 gggcggtccg caacgtcttc ggcaaactga atccgcaggg tgtcctggtc cattctttca
     3601 agattccgac agccgaagag ctggcacatg attttctatg gcggatacat gcccacgccc
     3661 cgaagaaggg gtttatccag atattcaacc gtagccatta cgaagacatc ctgatcaccc
     3721 gtgtgcacaa ccggatcgac gatgagaccg cccggttgcg gatgaaggcc attaatcatt
     3781 tcgag
//
LOCUS       DASYSV010000263         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1201875, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000263 DASYSV010000000
VERSION     DASYSV010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201875"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="VGQ51_08070"
     CDS             complement(<1..1683)
                     /locus_tag="VGQ51_08070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEV9036560.1"
                     /translation="MMPTADLKKPERHFLPNDYVLTDWNSLEPYFRDLEERPLNTRAD
                     LEKWLLDISELEAVISEDACWRQIKMTCDTENKAFEESFNYFCLEIQPKIQPYADRLN
                     RRLIENPHVTELDQREFFTYLRSVRKKIELFRESNIPLYAESSVLGQQFGVISGKMTI
                     DVNGQEYTLQQASRFLENPDRPLRETVYRTMQDRRLQDKDALNDLYSKLIAIRHRIAQ
                     NAGFANYRDYKFEELGRFDYNKQDCYAFHEAVREYVVPLVGKINARKRKKLGLDTLRP
                     WDGEAEPAGIQPLRPFTDGKDLLTKTTACFDKLNPFFGDCLRTMQQMGRFDLESRKGK
                     APGGYNCPLAETGAPFIFMNASGQMGDLTTMVHEGGHAIHSFLSHPLKLSSFKEYPME
                     IAEVASMSMELFSMDHWELFFDNKNDLARAREHQLERVISIFPWIATIDKFQHWVYEN
                     PGHSPEERKQNWLRILEEFSSRSVDWTGLEHYREFSWQKQLHLFEVPFYYIEYGIAQL
                     GAIGLWMQYKQNKEKALDNYMAALSLGGTRTLPELYAAAGLQFGFAKDYIKKL"
     gene            complement(1686..2405)
                     /locus_tag="VGQ51_08075"
     CDS             complement(1686..2405)
                     /locus_tag="VGQ51_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HEV9036561.1"
                     /translation="MEYNKIIAVTGLPGLYELLSSKADGAIVRSLDDKSTRFVSSRIH
                     NFSHLESIEVYTVRDNVNLVDILKAMEASSQKLPDEKDNEALRSYFGKVYPDLDFDRV
                     YVSDLKKMVKWFTVLKANDIEVKLTPPEEEPEAIAEAETTAAEEEKPKAKAAPKEKTA
                     PAPATAAPATAAKKKPEATEKKDTPAKEAKAGKSAKSAATPAKEKATAKKSAAAKDKG
                     EDKPKKPAAGSKTAKTSKKAK"
     gene            complement(2463..>3162)
                     /locus_tag="VGQ51_08080"
     CDS             complement(2463..>3162)
                     /locus_tag="VGQ51_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEV9036562.1"
                     /translation="LRQIFCTGRPMTIYYCYDAYCGWCYGFSPVIRRIYEEYRHVLAF
                     ETLSGGMILPETPQPIGVMAGYIQQAYKVVEERTGIRFGEDWLWHIFHPEESDWYPSS
                     EKPAIALCVFKDYYPDETIVFAGELQYALHVEGRDLTDDEAYRHLLGKYNIPADEFFG
                     KLRHPAYKEKAYDEFAMVRQLKVTGFPTVFLQVGESKFYLLARGYTDYETVKKGIDQV
                     LRESGESAGEAALQ"
BASE COUNT          676 a          857 c          758 g          871 t
ORIGIN      
        1 cagttttttg atataatcct tcgcgaatcc gaattgcaat cccgcggcgg cgtaaagttc
       61 cggcagcgtc cgggtgccgc ctaaggacag tgcagccata tagttatcta aagctttctc
      121 tttgttctgc ttatactgca tccataaacc gattgctccc aactgcgcga tgccatattc
      181 gatatagtag aacggcactt cgaagagatg caattgtttt tgccagctga attcccggta
      241 gtgctccaat ccggtccagt ccacggatcg agaggagaac tcttcgagta ttctcaacca
      301 gttttgcttt ctctcttccg gtgaatgccc cgggttctca taaacccaat gttgaaattt
      361 atcgatagtc gcgatccagg gaaagataga tatcactctt tcgagctgat gttcacgtgc
      421 tcgtgctaaa tcgtttttat tgtcaaagaa caattcccag tgatccatgc tgaagagctc
      481 catcgacatg ctggccacct cggcgatctc catgggatat tccttgaagg aactgagctt
      541 cagcggatga ctgagaaaag aatggatggc gtgtccccct tcatggacca tcgtcgtgag
      601 atctcccatc tgtccggagg cgttcatgaa aatgaacggc gcacccgtct ccgctaacgg
      661 acaattataa ccgcccggag cctttccctt gcggctttcc aggtcgaatc ttcccatttg
      721 ctgcatcgtc cgcaggcaat ccccgaaaaa ggggttcagc ttatcgaaac acgcggtcgt
      781 ttttgtcagc aagtcctttc catcggtaaa gggccgcagc ggctggatgc ccgcaggctc
      841 ggcttcacca tcccatggcc gcagcgtatc gagacccagc ttcttcctct tccgagcgtt
      901 gatcttgccc accagcggaa cgacatattc ccggacagcc tcatggaagg cataacaatc
      961 ttgtttgtta tagtcgaaac gacctaactc ctcgaactta taatcccggt aattggcaaa
     1021 acccgcattt tgcgcgatac gatgcctgat cgcgatcaat ttcgaatata gatcattcag
     1081 ggcgtcttta tcctgcagcc ggcggtcctg catcgtgcgg tacacggttt cacgtagagg
     1141 acgatcaggg ttttccagga acctggaggc ctgctgcagc gtatattcct ggccgttgac
     1201 atcgatcgtc atttttccag aaatgacccc gaattgctgt ccgagcacgc tcgattcggc
     1261 gtagaggggg atattcgatt cgcggaaaag ctcgatcttt ttccgtacgc tacgcagata
     1321 ggtaaagaat tcgcgttgat ccagctcggt gacatgcggg ttttcgatca gccgccggtt
     1381 gagccggtcg gcatagggct gtatcttcgg ttggatctcg agacagaaat aattgaagga
     1441 ttcctcgaac gctttgttct ctgtgtcgca ggtcatcttt atctggcgcc agcaggcgtc
     1501 ttcactgatc accgcttcca gctcgcttat atcgagcaac catttttcca ggtctgcccg
     1561 cgtattcagc ggtcgttcct cgagatctcg gaaatatggc tccaggctgt tccaatccgt
     1621 cagtacgtag tcgttgggta gaaaatgcct ttcgggcttt ttcagatcgg ctgtcggcat
     1681 cattctcact ttgctttttt ggacgttttc gcggtcttcg atccagccgc gggtttttta
     1741 ggcttgtctt cgcctttatc ttttgctgcg gccgattttt ttgccgtggc tttttctttt
     1801 gcgggcgtcg cggcagactt cgccgacttt ccggctttcg cttctttcgc gggcgtatcc
     1861 ttcttctccg tggcttccgg cttcttttta gccgccgttg cgggcgctgc cgtcgcaggt
     1921 gcaggtgcgg tcttttcttt cggagccgcc tttgccttcg gtttttcttc ttcggcagca
     1981 gtagtttcgg cttccgcaat ggcttccggc tcctcttcag gcggggtcag cttcacctcg
     2041 atatcgtttg ctttgaggac ggtaaaccat tttaccatct tcttcagatc gctgacatat
     2101 acccggtcga aatcgagatc aggatacacc ttgccgaaat aggatctcag agcctcatta
     2161 tccttttcat ccggaagctt ctgggaactg gcctccatag ctttcaggat atcgacgagg
     2221 ttgacattat cacgaaccgt atagacctcg atactttcga gatgcgaaaa attgtggatc
     2281 cggctggaga caaaacgggt ggacttatca tccagggaac ggacaatggc gccatcggcc
     2341 ttactgctca acaattcgta aagaccgggc aacccggtaa ctgctattat cttgttatat
     2401 tccatgcgaa cattttggga catcgaagtt aatgttaatg atttatttac ggacaatttt
     2461 tttcactgca aggctgcctc gccggcactt tcacccgatt cgcgcaatac ctgatcgatc
     2521 ccttttttca ctgtctcata atcggtgtat ccgcgcgcca gcaggtaaaa tttcgattcg
     2581 cccacctgta aaaatacggt agggaagccc gtcactttca attgacgcac catcgcaaat
     2641 tcgtcgtaag ccttttcctt ataggcgggg tgacgcaatt tgccgaagaa ttcgtcggca
     2701 gggatattgt attttccgag cagatggcga tatgcctcat cgtcggtgag atcgcgtcct
     2761 tcgacatgaa gggcatattg cagctcgccc gcaaatacga tcgtctcatc gggataataa
     2821 tccttgaata cgcacaaagc gatcgccggc ttctccgagc tcgggtacca gtcgctttcc
     2881 tccggatgga agatatgcca cagccagtcc tctccaaatc gtatgccggt cctctcctct
     2941 actaccttat aagcttgttg gatatatccc gccatgaccc ctatgggttg tggcgtctca
     3001 ggcaggatca tcccgcccga cagcgtctcg aaggcgagga catggcgata ttcctcgtag
     3061 atccgacgga tcaccgggct gaacccataa caccaaccgc aataagcatc gtaacaataa
     3121 tatatcgtca taggcctacc cgtacaaaaa atttgccgga ga
//
LOCUS       DASYSV010000264        12933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1969326, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000264 DASYSV010000000
VERSION     DASYSV010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969326"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VGQ51_08085"
     CDS             complement(<1..297)
                     /locus_tag="VGQ51_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036563.1"
                     /translation="MRNLIFACIFICLASTASAQSEKARTEKPQTAGPSFVKVTGEVR
                     TPLTLAAADIAAMPRTTVSAKDKQGAAHSYTGVALSDIFKKAGVTMGSQLRGKNM"
     gene            complement(313..873)
                     /locus_tag="VGQ51_08090"
     CDS             complement(313..873)
                     /locus_tag="VGQ51_08090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEV9036564.1"
                     /translation="MKVLLFNGCSDPRPESTGRRISAYLHRQLIEKGFSVSQVNVCDA
                     QIPFFELRQPHPVAAVEDMCEAFLQSDLQVWLTPLYHGGMTGAMKNCLDWLELTSRLE
                     KPYLTDKVVGLVCWADGGQAMQGINAMDAVAKALRAWVIPYTVALVKDQLYTDAHRRE
                     LAVEHTRKLDRLLGLLGNARQSLALT"
     gene            899..1480
                     /locus_tag="VGQ51_08095"
     CDS             899..1480
                     /locus_tag="VGQ51_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV9036565.1"
                     /translation="MFDEIDQFVSKYVTLTGDEREFFHSLLRHRSFRKKQFLLKEGEI
                     CDFEAFILKGCIRTYYLDKEGVETILLFAVEEWWVSDIISFSERVPSNLFIETIENSE
                     LLTIDYAAKKLLFERVPAFERMFRLLLQRSLGVLQQRFYASVSQTAEERYRQFLEKYP
                     QVAQRVPQHQIARYIGVSPEFLSKIRSGMYRKH"
     gene            complement(1555..2187)
                     /locus_tag="VGQ51_08100"
     CDS             complement(1555..2187)
                     /locus_tag="VGQ51_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV9036566.1"
                     /translation="MHTLQYITGGTDIGTADKALILVHGRGGSAEDILSLADALPVED
                     YALFAPQAAANSWYPYSFMAPLSNNEPWLTSALGVLKQLVAHIGSKGIAADHTWFAGF
                     SQGACLMLEYLARNAERYGGAAAFTGGLIGDRIYPQHYRGDFGGTPVFIGTGDPDPHV
                     PVQRVHDTVSLLKGMNAAVTEKVYSGMGHTISGDELRQASRLVLGRELSA"
     gene            complement(2196..3203)
                     /locus_tag="VGQ51_08105"
     CDS             complement(2196..3203)
                     /locus_tag="VGQ51_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEV9036567.1"
                     /translation="MKNNISGIHHITAIAGNAQRNYDFYTRTMGLRMVKRTVNFDDPG
                     TYHFYYGNETGTPGTILTFFPWEGIAQGRTGTGLATEIGYSVPAGSLEFWAQRFREHK
                     IKSGEVAERMGETYLPFADPDGLNFSLIVPGTPDSRTAWKTPEIGDAVATKGFHSVTL
                     TLKNIAPTVEVLTDIFGYTLQRREGNRYRFVAAASAASASPAAGNAAAGNASTAASLA
                     PTASIVDIIEAPREIRGLNGSGTNHHVAFRVADEATQAEFREKILLKGLHITPKINRD
                     YFYSVYFREPGGVLFELATDDPGFTVDEPKAELGSHLKLPVQYESMRDSIEKGLPALH
                     L"
     gene            complement(3333..5540)
                     /locus_tag="VGQ51_08110"
     CDS             complement(3333..5540)
                     /locus_tag="VGQ51_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase"
                     /protein_id="HEV9036568.1"
                     /translation="MKGVRISILFCLAIVPGGVIKAQLDKAASYALIARLIPTHAAEF
                     TIEEIPSLRDTFEIASRGNQIVLRGNNGVSIASALYDYLQTFCHCQVTWDGTNLHLPA
                     KLPPVTHVIRRSSPYRYRYYLNYCTFNYSMSWWDWPRWEKEIDWMALHGIDMPLAITG
                     EEYTWKEVYRELGFSDRELDSFFCGPAWFAWFWMGNLDRWGGPLSQHWMESHRDLERR
                     ILRRERQLGMKPVLAAFTGHVPASFAKHFPGARLKATNWKNGFADTYILDAADPLFAM
                     IGKKFLEAQTRLYGTDHFYSADTFNENEPPSDDPDFLAALSRRVYEGMHEADSSAIWV
                     MQGWLFYSDRKFWKPAQIKALLDAIPDEHMLLLDLAAEIAPVWKRTDAFYGKPWIWNM
                     LNNFGGNVNLFGRMDGVAEGPAAALNDTSSGKLTGIGLTMEAIEQNPVIYELMMDNTW
                     RSQPIDLHEWLAGYVRNRYGVEDSLLVKAWEILRQTVYNGRLIRDGAESIITGRPTLD
                     SSTVWTQTKLNYPAPDLLPAWDLFMRAAQRPAQTDGFLFDLTDITRQVLANYALPLQR
                     EWVGAYRSRDSVAFTRFSHQYLELIEDMDRLLATRKDFLLGRWIGAARARGATPAEKH
                     LYERDAKDLITLWGDANSPLHEYANRQWSGLLDGFYKPRWEQFFRLLQSSLQKGVTPD
                     LAGFEKNIRHWEWNWVNEEHVFPVAPRGDSRQVAIALYRKYRQRLSDAYTRRF"
     gene            complement(5537..5764)
                     /locus_tag="VGQ51_08115"
     CDS             complement(5537..5764)
                     /locus_tag="VGQ51_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEV9036569.1"
                     /translation="MATTKITVKSNGSLKLEGDFEIVDGNGMLYGLGGREIISICRCG
                     LSKNKPFCDGSHKGHFEHDATAFDLPPKPQP"
     gene            5904..7058
                     /locus_tag="VGQ51_08120"
     CDS             5904..7058
                     /locus_tag="VGQ51_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV9036570.1"
                     /translation="MEYDNDRPRKRAPKAEIFYWILLLLVQPMTNSLTVFIDSPGAWV
                     QLLLISLLVFPAYIIYSRIMGLALTTSGRKVFPILLTVGFFLIIQVFLLAIYSLMLKF
                     PLTIGQHFYYSLQSRTMIREAWWTAFNMAFSIAVFYIRKADDEKDMLETMKKDNTFFR
                     LRYLRAQLNPHFLFNTLNSIYSMSMQKSDRTPEMVVRLADIMRYLIYECNEEKIPLDK
                     EIEFIRNYIEIEKTRYNADIRFTVEGMTTGIMIEPFLFISFIENGFKHAMDHTFNEPF
                     IYITLRVGSGQLVLNVLNSTNTELEMQAQRINGMGISHSRSLLELLYPASYDLDIIQT
                     EKDETRTSELRLRNARERLESLYPDSHTLDVILNKSTFTVSLILKTIAAC"
     gene            7052..7771
                     /locus_tag="VGQ51_08125"
     CDS             7052..7771
                     /locus_tag="VGQ51_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9036571.1"
                     /translation="MLKCIIVEDETLAQDVIKSHITRIGRMELVGVYRTAPEAKDALD
                     RRDVDVLFLDIRLPGMSGLHFLRTLSNPPLVILTTAYAEYALESYEFNVIDYLLKPIS
                     YERFARAVNKILDGRLYSFTATGDPAAMPMTGPQGDHIFVKSNSKFFRVDFSEILYVQ
                     GMKDYLKIVTPDFTLVTHQTMNEMEAALPPRQFIRVHRSWLVAVRHIRAIYGNSIELG
                     KLTIPIGTNYKTTVMELIGKK"
     gene            7860..9494
                     /locus_tag="VGQ51_08130"
     CDS             7860..9494
                     /locus_tag="VGQ51_08130"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV9036572.1"
                     /translation="MDRRKFLRNGSLAGLGLSSIHLSSPHEARTATAEETTGPVAAEF
                     PLLEATIDQLQQMMQSGQHDSQSITKLYLRRIEAIDKKGPALNAIIELNPDALSIAAA
                     MDAERKAGKIRGPLHGIPVLVKDNIDTGDKMQTTAGALALEGHHAAKDAFIIGKLRAA
                     GAVLLGKTNLSEWANFRSSRSTSGWSSRGGQTKNPYFLDRNPSGSSAGSGSAVSGNLC
                     AVAIGTETNGSVVSPSSINGIVGIKPTVGLLSRSGIIPISSTQDTAGPMARSVKDAAF
                     LLAALQGFDPEDTVTDTSQGKTRPDYNASFDAGSLKGKKIGIEKAALSGHEGVVALFQ
                     EAIGVLKAQGATVVEIELQRLLTDPNSVEGIVLQYEFKHGVDAYLATAGASVRSLADV
                     IAFNKANEQRAMPFFKQETLERSEARGSLDNPEYTEALKKLLTARTIIDDLMKQNQLD
                     ALVAPTSGPACCIDLVAGDYGTGRSFSGPAAIAGYPHVTVPMGLVFDLPVGLSFVGTA
                     YTEPSLIGMAYAYEQASKKRVPPGMVNAANPGRPTV"
     gene            complement(9522..11720)
                     /locus_tag="VGQ51_08135"
     CDS             complement(9522..11720)
                     /locus_tag="VGQ51_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV9036573.1"
                     /translation="MIKKAALSAFLSCIFSMVFAQQRYGSLAEALQTGFRLHGRPGPR
                     SVNWINNGDQYGFENSGDIRTMDPKTLQEKTIFSTGGLHFPGTTTPFKYESFQWSQDS
                     KHLVFRTNFRHIYRNSGISDYYIYDIEGQGLRQAAKDARSAQLSPDGSSVGIEREGNL
                     FVYDFATNAERQLTHDITSDTGIFSGHYDWVYEEEFGQGQAWNWSKDSRYIAFWQFDE
                     HRVPLFQMTNFEGLHSEQVHIPIPQPGDPNPAVRIGVIDVHSGSKVWLTPDETGDYYI
                     PRIYWTSDPDVLALMTLNRAQNHMKLYFFNVKTGEHHVVLQEQNNTWVSIFDFYTEVN
                     DMVYFPENTRDFFWVSDRSGYYHIYHYNYEGRLINAVTKGNWDLIKVTGINPKTKTIY
                     YLSAEASPLEQQLYSIRYDGSGKRRLTQTPGSRRIDMSPNTAWYIEEFSNVNTPLEVG
                     LYNAQGKLLKMLEDNHGVSEFIRTHAYSPMQLFRFRTTDTADIDGYMIKPFDFDSSKR
                     YPVVMQVYGGPESHDVSDRWASDGFQQWLAQNGYIVVGINNRGVADYGSAFMKIVYRQ
                     LGKWESHDFAEAARYLATLPYVDGANIAIMGTSYGGYSTTYTLLTHPGVFKVGIANSP
                     VTDWRLYDDVYTERYMGLLPDNEAGYKKSADMTYAANLKDHLLLIHSMSDDNVHPANT
                     MQLLTALTNAGKDVDLRIYPPGAHGAAYNWQSYLLINTVSYEYLERYLKH"
     gene            12049..>12933
                     /locus_tag="VGQ51_08140"
     CDS             12049..>12933
                     /locus_tag="VGQ51_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036574.1"
                     /translation="MKKFTLILWGIFYLVPSFAQTATPDTTTTLSSNKDIRNKVLSDI
                     QDNLDKKTKNIDQTLNKLDNKVDSLDKEIAASRDARDKADKLLLRVQALETKQQAIEQ
                     NELYVYQANYQSAIINLVSMDKEIKPLVLFNASKSFFSTLTETADPMKYPGYQDWYKK
                     FYQYIQKEKDHEPSLAVLNDMVQITGNLSAATPIAGPVSQALFLGIDSYINSMGSKKK
                     ELRDQSQKMFELTAKLSQFDYDKDLIEQQWEIVTKELEKLQIHYDTILNQNLALLKVP
                     SAQYQQDFNRQTDADKRYQ"
BASE COUNT         3035 a         3514 c         3450 g         2934 t
ORIGIN      
        1 catgttctta cctcttaatt gggaccccat ggtcacgcca gcctttttga aaatgtccga
       61 aagggcgaca ccggtatagc tatgcgccgc cccctgtttg tctttggcac tgacggtggt
      121 ccggggcatt gcggcgatat ctgcggcggc gagggtcagc ggtgtccgga cttctcccgt
      181 aacttttacg aaggaaggac cggcggtttg cggcttttcg gtccgggcct tttcgctttg
      241 ggccgatgcg gtactagcca gacaaataaa gatgcaggcg aagatgaggt ttctcattat
      301 cgggttgatg ttttaggtga gggctaggga ttgccgggca ttgcccagca aaccgagcag
      361 tctgtccagt ttgcgcgtat gctcgacggc cagctcgcga cgatgggcat ccgtatacaa
      421 ctgatccttc accaatgcga cggtataagg aataacccag gcgcgaaggg ccttggctac
      481 ggcatccatg gcgttgatcc cctgcatggc ctgtccgccg tcggcccagc aaacgagccc
      541 cactaccttg tcggtaagat aaggtttttc cagacgcgac gtgagctcca gccagtccag
      601 acaattcttc atcgcgccgg tcatcccccc gtgatacagc ggagtcagcc atacctgaag
      661 atcggattgc agaaaggcct cgcacatatc ctctaccgcg gccaccggat gtggctgacg
      721 caattcgaag aacgggattt gtgcatcgca gacgtttacc tgggagacgg aaaatccctt
      781 ttcgatgagt tgccggtgca gataggcgga tatcctgcgg ccggtggatt cgggacgcgg
      841 gtcactgcaa ccgttgaaga gcagcacttt catgtgctga agttacgtaa tttgcggaat
      901 gttcgatgag atcgatcagt ttgtgtccaa atacgttacc cttacggggg acgaacggga
      961 gttctttcat tctctgctcc gacatcgcag cttccggaag aaacaattcc tgctgaaaga
     1021 gggagagatc tgcgatttcg aagcgttcat ccttaagggt tgcatccgga cgtactacct
     1081 tgataaggag ggggtcgaaa ccatcctgct tttcgcggtg gaggaatggt gggtcagcga
     1141 tatcatcagc ttctccgaac gggtgccgtc caatcttttc atcgagacca tcgagaacag
     1201 cgagctgctc accatcgatt atgcggcaaa gaagcttctg ttcgagcggg tgcccgcttt
     1261 cgagcggatg ttccggctat tgctgcagcg atcactgggt gttttacaac aacggttcta
     1321 tgcctccgtg tcccaaacgg cggaagaaag atatcgccag ttcctggaaa agtatccgca
     1381 ggtggcgcag agggtgccgc agcaccagat agcaaggtat attggcgtat cgccggaatt
     1441 cctcagcaag atcagatcgg gcatgtacag gaaacattga acgttccggc cccgcccctc
     1501 gccgactact cccgctcatg ccgacagttc ccgcccctcc ccgactactc ccgctcatgc
     1561 cgacagttcc cgccccagca ccagcctgct ggcctggcgc aattcgtcgc ccgagatcgt
     1621 atgccccatc ccggagtaga ccttctccgt tacggcggca ttcatcccct tcaggaggct
     1681 aacggtatca tgtactcgct gaaccggcac atgcggatcg gggtcgcccg tgccgatgaa
     1741 aaccggcgtc ccgccaaaat ctccccggta atgctgcgga tagatcctgt cgcctatgag
     1801 cccgccggtg aacgctgccg ctccgccata acgttcggca ttgcgcgcaa gatattccag
     1861 catcagacag gcgccttgcg agaaacccgc gaaccaggta tgatcggcgg cgatcccttt
     1921 cgacccgata tgcgcgacca gttgtttcag caccccgagg gcgctggtca gccagggttc
     1981 attattggac agaggagcca taaaggaata aggataccag gaattggcgg cagcctgcgg
     2041 ggcgaataag gcgtaatctt ctaccggcag ggcatccgcc agcgaaagga tgtcttcggc
     2101 tgaaccgccg cggccgtgca caaggatcag ggccttatcc gccgtcccga tgtcggtgcc
     2161 accagtgata tattgtaaag tatgcatgtt gtgttttaaa gatgaagcgc cggaaggcct
     2221 ttttcgatgc tgtcccgcat ggattcgtac tggacaggca atttcagatg actgcccaat
     2281 tcggctttcg gttcatcaac ggtaaatccc ggatcatcgg tagccagttc gaacaacacc
     2341 cctcccggct cccggaaata caccgagtaa aaatagtccc ggttaatctt tggggtgata
     2401 tgcagtccct tcaggagtat cttttcacgg aattccgcct gggtggcttc gtcggcaacg
     2461 cggaaggcga cgtggtgatt ggttccgctg ccgttgagac cgcgtatttc acggggcgcc
     2521 tcgataatat ccacgatgga agcagtgggg gcgagactgg cagcagtgga ggcgttcccg
     2581 gcggcagcgt tcccggcggc ggggctggcg gaggcggcac tggcggcggc cacaaaacga
     2641 taccggtttc cttcccgccg ttgcagcgta tatccgaaga tatccgtgag gacctctacc
     2701 gtgggagcga tgtttttcaa tgtcagggtc acgctgtgaa aacctttggt agccacggcg
     2761 tctccgatct ccggtgtctt ccaggctgtg cggctgtcag gtgtccccgg aacgatcagg
     2821 ctgaaattca gcccgtcggg gtccgcgaat ggaagatagg tctcccccat cctttcggcc
     2881 acttccccgg atttgatctt atgttcccgg aaacgctgcg cccagaactc caggctgccg
     2941 gcaggtaccg aatagccgat ctcggtagcg agaccggtac cggtccttcc ctgcgcgata
     3001 ccttcccacg gaaaaaacgt gagtattgtt cccggagtac ccgtctcatt tccataataa
     3061 aaatgataag tacccgggtc gtcgaaattg acggtgcgtt tcaccatccg gagacccatc
     3121 gtcctggtat aaaaatcgta gttgcgttgg gcattgcccg cgatagcggt gatatgatgg
     3181 atgcccgata tattgttttt catacagtgt aaagttacgg tccctgaggg gcgtgatcca
     3241 ttaacttagt ttaagaaacg ggggtatagg gcgctggacc gggcatagtc atcagaaacg
     3301 gtgcgatagg gcgctggcac ggcggcatgt catcagaaac ggcgggtata ggcatctgat
     3361 aagcgctggc ggtatttccg gtagagggca atggccacct gccggctgtc ccctctgggt
     3421 gcaacgggga agacatgttc ttcgttaacc caattccatt cccagtgtcg gatattcttt
     3481 tcaaaaccgg cgagatcagg ggtgacgccc ttctgtaagg atgattgcag cagccggaaa
     3541 aattgctccc accggggttt atagaacccg tcgagcaaac cgctccattg gcggttggca
     3601 tattcatgca aggggctgtt agcgtcgccc caaagagtga taagatcttt ggcatccctc
     3661 tcgtaaagat gcttctccgc gggggtggcg ccccgggccc gggctgcgcc gatccaccga
     3721 cccagcagga aatccttacg ggtagccagc agccggtcca tgtcttcgat caattcgagg
     3781 tattgatgac tgaaacgcgt aaaagcgacg ctgtccctgc tacggtaggc cccgacccat
     3841 tcgcgttgca gcggaagggc ataattggcc agcacctggc gggtgatgtc ggtcagatcg
     3901 aagaggaatc cgtctgtctg agcagggcgt tgcgccgcgc gcataaaaag atcccatgcc
     3961 ggcagaaggt ccggcgccgg atagttgagt ttggtttggg tccagacggt ggaagagtcg
     4021 agcgtcggcc ggcctgtaat gatcgattcc gcaccatccc ggatgaggcg gccattgtag
     4081 accgtttggc gaaggatctc ccaggccttc acgagcaggc tgtcttcgac gccatagcgg
     4141 ttgcggacat acccggccag ccactcgtgc agatcgatgg gttgcgaccg ccaggtattg
     4201 tccatcatca gctcgtaaat cacggggttt tgttcgatcg cctccatcgt gaggccgatg
     4261 ccggttagct ttcccgaaga cgtatcgttg agcgccgccg cgggcccttc cgcgacaccg
     4321 tccatccttc cgaatagatt cacattccct ccgaaattat tcagcatgtt ccatatccat
     4381 ggctttccat aaaaggcgtc cgttcttttc caaaccggtg cgatctccgc ggccaggtcc
     4441 agcaggagca tgtgctcatc gggtatggca tccagcagtg ctttgatctg tgccggcttc
     4501 cagaatttcc ggtcactata aaataaccag ccctgcatga cccatatggc tgacgagtcg
     4561 gcctcatgca taccttcata cacgcgccgg ctcaacgctg ccaggaaatc cgggtcatcg
     4621 gacggaggtt cgttctcatt gaaggtgtcg gcggaataga aatgatcggt accatagagc
     4681 cgcgtttgcg cctccaggaa tttctttccg atcatcgcaa aaagagggtc tgccgcatcc
     4741 aggatatagg tatcggcaaa accgttcttc caattcgtag ctttcaaccg tgcacccgga
     4801 aaatgtttcg caaacgaggc gggcacatgc cccgtgaagg ctgctaagac cggtttcatt
     4861 cccagttgtc tctcgcgtcg aaggatccga cgttccagat cacgatggct ttccatccaa
     4921 tgttgtgaca agggaccacc ccatctgtcc agattgccca tccaaaacca agcgaaccag
     4981 gccggtccgc aaaaaaagga atccagttcc ctgtccgaaa agcccaattc cctgtaaact
     5041 tccttccagg tatattcctc gccggtaatg gccagcggca tatcgatgcc atgaagggcc
     5101 atccagtcga tctccttttc ccatcgcggc cagtcccacc agctcatgct gtagttgaag
     5161 gtgcaatagt tgagataata ccggtagcga tagggcgaag atcttcggat gacatgcgtt
     5221 accggcggca gtttggccgg gagatgaagg ttggtgccgt cccatgtgac ctggcaatga
     5281 caaaaggttt gcagataatc gtagagcgcg gaggcgatgg ataccccgtt gttgcctctt
     5341 aacacgatct ggttgccgcg actggcgatc tcgaaggtat cgcgcaggga agggatctcc
     5401 tcgatggtaa attcggcggc atgcgtgggg atcaggcgtg cgatcagcgc gtaggaagca
     5461 gccttatcca gctgagcttt tatgacgccg cccggcacta tggccagaca gaatagtatg
     5521 ctgatgcgta cccccttcat ggctgaggct tgggcggcag atcgaatgca gtcgcatcat
     5581 gctcgaaatg acctttatgg ctgccatcgc agaacggttt gtttttggaa agaccgcaac
     5641 ggcagatact gatgatctcc ctgcccccca ggccatagag catcccgttg ccatcgacga
     5701 tctcgaaatc gccttccagt ttgagcgagc cattgctttt gacggtgatc ttggttgtgg
     5761 ccatggtact tcttttttgc cgccaaaaat agcgaaaaac gacatccatg gcgcagattc
     5821 atcgaacctc cgcaggaatt agtcgaaatc ttttcacttc gggccggtct tgtattagct
     5881 ttatagagcc tataaaaccc accatggaat acgacaacga cagaccccga aaacgcgctc
     5941 caaaggcgga gatcttttac tggatcttac tgctgctggt ccagccgatg accaattccc
     6001 tgacagtttt catcgatagc ccgggcgcct gggtgcagct gctgctgatc agcctgctgg
     6061 tctttccggc ctatatcatc tattcacgga tcatgggtct ggcgctgaca accagtggac
     6121 gaaaagtatt cccgatcctg ctgacagtgg gatttttcct catcatccag gtcttccttc
     6181 tggccatcta ttccctgatg ctgaagttcc cgctgaccat cggacaacac ttctattaca
     6241 gcctgcaatc ccgaaccatg atccgtgagg cctggtggac ggcgttcaat atggcctttt
     6301 cgatcgccgt cttctacatc cggaaggcgg atgacgaaaa agacatgctg gagaccatga
     6361 aaaaggacaa tacctttttc cgtctccggt atctgcgggc gcaactgaac ccgcatttcc
     6421 tgttcaatac gctgaacagc atctattcga tgagcatgca gaagtcggac cggacgccgg
     6481 aaatggtggt ccggctcgcg gatatcatgc gctacctgat ctatgagtgc aacgaagaaa
     6541 agataccgct ggacaaggag atcgagttca tccgcaacta tatagagatc gaaaagaccc
     6601 gctataacgc cgatatccgg ttcacggtgg aaggcatgac cacgggaatc atgatcgagc
     6661 ccttcctgtt catctctttt atcgaaaacg gctttaaaca cgccatggat cacaccttca
     6721 acgagccctt catctatatc acccttcggg tgggcagtgg tcagctggtc ctgaatgtcc
     6781 tcaacagcac caataccgag ctcgaaatgc aggcacaacg catcaatggt atgggcatct
     6841 cgcacagcag gagcctcctg gagctgctct accccgcctc ctacgatctg gatatcatcc
     6901 agaccgaaaa agacgaaacg cgcaccagcg agctgcgttt acgcaatgca cgcgagcggc
     6961 tggaaagcct ctatcccgat tctcatacct tagatgtaat tttgaacaag agtacgttca
     7021 ccgtatcgtt aattttaaaa accatcgctg cttgttgaaa tgcatcatcg ttgaggacga
     7081 gaccctggcc caggatgtca tcaagagtca tattacgcgt atcgggcgga tggagctggt
     7141 gggtgtatac cgcacggcgc ccgaggcaaa ggatgcgctg gaccgacgcg atgtagatgt
     7201 cctcttcctc gatatccgcc tgcccggcat gagcggcctt cattttcttc gtacactctc
     7261 caacccgccc ctggtaattc tgaccaccgc ctatgccgaa tatgcgctcg aaagctacga
     7321 gttcaacgtg atcgattatc tcctcaaacc catttcctac gagcgatttg ccagggcagt
     7381 gaataagatc ctcgatggtc gtctctattc tttcacagcg accggcgatc ccgcagcgat
     7441 gccaatgacg ggtccgcagg gcgatcatat cttcgtcaaa tccaacagta aattcttccg
     7501 cgtcgatttc tccgagatcc tctatgttca gggtatgaag gattacctga agatcgtcac
     7561 gcccgacttc acgctcgtca cgcaccagac catgaatgaa atggaagctg cacttccccc
     7621 ccggcaattc atccgggttc accgcagctg gctcgtggcg gtgcgtcata ttcgcgctat
     7681 ctatggtaat tccatcgagt tggggaagtt gacgataccc atagggacaa attataagac
     7741 gacggtgatg gagttgatcg gaaagaagta gactcacttt cttttttaat agcggcccca
     7801 gcgcgatgcg cgggggcctt tttttcttat tttcaaccgt taaatctaaa cttctgcata
     7861 tggaccgtcg caaattcctt cgaaatggct cgctagccgg actcgggttg tcttccattc
     7921 atttatcatc tccccatgaa gcaagaaccg ccacggcgga agagacgaca gggccggtag
     7981 cggctgaatt tcccctgctg gaagcaacca tcgaccagct tcagcagatg atgcaatcgg
     8041 ggcagcatga ttcccagtcc atcaccaaat tatacctccg acgcatcgaa gccatcgaca
     8101 aaaaaggccc ggccctcaat gccatcatcg aactcaatcc cgatgcattg tcgatagccg
     8161 cggcgatgga cgcggaaaga aaggccggaa agatcagggg ccccctgcac ggtatccctg
     8221 tccttgtcaa agacaatatc gatacgggcg acaaaatgca gacgaccgcg ggggcgctcg
     8281 cgctggaagg gcatcacgct gccaaagacg cattcatcat aggaaaactg cgggctgcgg
     8341 gcgctgtact gcttggaaag accaatctca gcgaatgggc gaatttcaga tcgagccggt
     8401 cgaccagcgg ctggagcagc cggggaggcc agacaaaaaa cccgtatttc ctggaccgga
     8461 acccatccgg gtcgagtgca ggctcgggtt ccgcggtgtc gggcaatctc tgcgcggtgg
     8521 ccatcgggac tgagaccaac ggttcggtcg tttcgccttc ttccatcaat ggaatcgtgg
     8581 gtatcaaacc cacggtagga ctgctcagcc gcagcgggat cattcccatt tcttcgaccc
     8641 aggataccgc ggggccgatg gcaaggtcgg taaaagacgc agccttcctg cttgccgcgc
     8701 tccaggggtt cgatcccgaa gatacggtca ccgataccag ccagggcaag acaagacctg
     8761 attataacgc atctttcgac gcaggcagtc tgaaagggaa aaagattggt atcgagaaag
     8821 cggcgctttc aggtcacgaa ggcgtcgtcg cactgttcca ggaggctatc ggggtgttga
     8881 aagcgcaagg cgccaccgtc gtggagatcg agctgcagcg gttgctgacc gatcccaatt
     8941 ccgtggaagg catcgtcctg caatacgaat tcaaacacgg tgtcgacgcc tatttggcta
     9001 ctgcgggtgc atccgtcagg tcgcttgcgg acgtcatcgc attcaacaag gccaacgaac
     9061 aacgagcgat gccttttttc aaacaggaaa ccctcgagag aagcgaggcg cggggaagtc
     9121 tcgataatcc cgaatatacg gaagcattga aaaaactgct gacagcgcgc accatcatag
     9181 acgatctgat gaaacaaaat cagctggatg cgctggtcgc gcctacaagc ggacctgcgt
     9241 gttgcatcga cctggtcgcc ggcgattatg gcacgggccg gtcgttctcg ggacccgcgg
     9301 cgatagccgg ttacccacat gtcacggtgc cgatgggact ggttttcgat ctgcctgtgg
     9361 gactgtcatt cgtcgggacg gcctataccg aaccaagcct gatcggcatg gcctatgctt
     9421 atgaacaggc gtcgaaaaaa cgggtgcctc ccggtatggt gaatgcggcg aatccgggta
     9481 gacctacggt ctaaggtcag ggaccgctga gcgaatccgg cttaatgttt caaataccgc
     9541 tcgagatatt catagctgac ggtattgatc agcagatagc tctgccagtt ataagcggct
     9601 ccatgcgccc ccggcggata tatcctcaga tcgacatcct tcccggcatt ggtcaacgcg
     9661 gtcagcaact gcatagtatt cgcgggatgg acattgtcat cgctcataga atggatcagc
     9721 agaaggtgat ctttcaggtt ggctgcatag gtcatgtccg cgcttttctt atagcccgcc
     9781 tcattgtcgg gtagaagtcc catatatctt tccgtataca cgtcgtcata aagtcgccaa
     9841 tcggtgacag gtgaattggc gatcccgact ttgaaaacgc cgggatgggt cagcagcgta
     9901 taggtggtgc tgtagccgcc ataactggta cccatgatcg ctatattagc gccgtcgaca
     9961 taaggaagcg tcgcgagata gcgcgcggct tcggcgaaat catgactttc ccatttgccc
    10021 agctgccggt agacgatctt cataaatgcg ctgccataat cggcgactcc acggttgttg
    10081 atacctacga cgatatagcc gttttgcgcc agccattgct ggaagccgtc gctggcccac
    10141 cggtcggata cgtcatgaga ttccgggcca ccgtacacct gcatgacgac cggatagcgt
    10201 ttcgaagagt cgaaatcaaa cggcttgatc atataaccgt cgatatctgc agtgtccgtg
    10261 gttcgaaatc ggaacaactg catcggggaa taagcatgcg tacggataaa ctcgctgacc
    10321 ccatgattgt cttcaagcat ttttagcagc ttgccctgag cgttatacaa cccgacttct
    10381 aaaggagtgt tcacgttgct gaactcttcg atataccagg ccgtattcgg cgacatatcg
    10441 atcctgcggg aaccgggggt ctgcgtcagc cgccttttgc cgctgccgtc ataccgaatg
    10501 ctgtacagct gctgctccag cggagacgcc tccgccgaca gatagtagat ggtctttgtc
    10561 ttcggattga tgccggtgac tttgataaga tcccagttgc ccttcgtgac cgcattgatc
    10621 agccgtccct cataattata atgatagata tggtagtagc ccgagcggtc ggacacccaa
    10681 aagaagtcgc gggtattctc ggggaagtag accatatcgt tcacctccgt ataaaaatcg
    10741 aaaatggaga cccaggtatt gttctgttct tgcaatacaa catgatgctc gccagtcttc
    10801 acgttgaaga aatacaattt catgtggttt tgtgcccggt tgagcgtcat cagcgcgagt
    10861 acatccggat cgctggtcca atagatgcgc gggatataat aatcccccgt ctcgtcgggc
    10921 gtcaaccaga ccttgctacc ggagtgcacg tcgatcaccc cgatgcgcac tgcgggattg
    10981 ggatcgccag gctgtggaat gggaatatgg acctgttcgg aatgcaggcc ttcgaaattc
    11041 gtcatctgga atagaggcac gcggtgttcg tcgaattgcc agaaggcgat ataacggctg
    11101 tccttcgacc agttccatgc ctgaccctgt ccgaattctt cctcgtatac ccagtcatag
    11161 tggccgctga aaatgccggt atcgctggtg atgtcatggg tcagttgccg ctcggcattc
    11221 gtggcgaaat cataaacgaa aagattgcct tcgcgttcga tacccaccga agatccgtca
    11281 ggggataatt gcgccgaacg cgcatccttc gccgcctggc gtaatccctg accttcgatg
    11341 tcgtaaatat agtagtccga tatacccgaa tttctataga tatgccggaa atttgtccgg
    11401 aatacaaggt gtttcgagtc ttgcgaccat tggaaagatt cgtatttgaa aggggttgtc
    11461 gtaccgggga aatgcaggcc gcccgtgctg aagatcgttt tctcctgcag cgtttttggg
    11521 tccatggtgc ggatatcgcc cgaattttca aacccatact gatcgccatt attgatccag
    11581 ttgacgctgc gcgggcccgg gcgaccatgt aatctgaaac ccgtttgtaa tgcttcggca
    11641 agactgccgt accgttgctg agcgaatacc atcgaaaata tgcaggagag aaatgccgat
    11701 aaagccgctt tttttatcat ccggggtgga atattagacg gctaaagtaa aacattctga
    11761 gggaaaaggg tggaaacgaa aaaaccccca agtttaatca ggggcttttc aagagagatt
    11821 gaattttaag gctttcgatt agaaaatgaa agccaatttg gtttttcagg atcttgtgat
    11881 aagggtattc attggtattg gatgattcaa agctacgtga caacggcttg ttaaaaaaga
    11941 gcaaagaccc tcgatttcta tcgtattttt acgattctaa ttgcattcta atgcacagtt
    12001 tcaggcaccg taggctaaat ttactgtaag ccaaaaaatt ccaatcactt gaaaaaattc
    12061 actttaatcc tgtggggcat cttctatcta gtcccatcct tcgctcagac ggcaacaccc
    12121 gatactacca ctactctttc ctccaataag gacatccgga acaaggtcct cagcgatatc
    12181 caggataacc tggacaaaaa gaccaaaaat atcgaccaaa ccctcaacaa attagacaac
    12241 aaggtagaca gtctcgacaa ggagatcgcg gcctcacggg acgcgcggga caaggcggac
    12301 aaactcctgc tgcgggtaca ggcgctggag acaaaacaac aggccatcga gcaaaatgaa
    12361 ctctatgttt accaggcaaa ctaccagtcc gcgatcatca atctcgtttc aatggataag
    12421 gagatcaagc cactggtcct tttcaatgcc tcaaagagtt ttttctcgac gctcaccgag
    12481 accgccgacc cgatgaaata tcccggctac caggattggt ataaaaaatt ctatcaatat
    12541 atccagaagg aaaaagacca tgagccgtca ttggccgtac tcaacgatat ggtgcagata
    12601 acgggtaatc tttcggccgc gacacctatc gccggacccg tctcgcaggc cttgttcctt
    12661 gggatcgatt cgtatatcaa ttcgatgggc tcgaagaaaa aagagttgcg cgatcaaagc
    12721 cagaaaatgt tcgagctcac cgccaagctg agccaattcg attacgacaa ggacctgatc
    12781 gagcaacagt gggagatcgt gacgaaagag ctggaaaagc tgcagatcca ctacgatacc
    12841 atcctcaacc agaatctcgc actgttgaaa gtgccgtccg cacagtatca gcaggacttc
    12901 aaccgccaga ccgacgcgga caaacgttat caa
//
LOCUS       DASYSV010000265         3648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1455902, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000265 DASYSV010000000
VERSION     DASYSV010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455902"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2482
                     /locus_tag="VGQ51_08145"
     CDS             <1..2482
                     /locus_tag="VGQ51_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEV9036575.1"
                     /translation="AGEDDCLLAVTSTSFDISVLELFWTLCNGIQVVIHPSGISFSGL
                     DRYLDHEAGRVHRPVTMLQSTPSFMRMVREGEGSRVFLRSLRRLLLGGEGVPLSLVKG
                     LQRDTGAGIYNMYGPTETTIWSCMHDFGGEEEKVVVGGPMVNTQVYILSRELRLVPVG
                     VAGDVYIGGEGLSRGYWRRPELTAERFIAHPYRSGQRLYKTGDIGRWVRGGRIELLGR
                     EDHQVKIRGHRIELGEIERVLSQREQTGEVVVVAREREGDKLLTAYYTGEAGGEELRT
                     YLSGRLPEYMVPSYFMRLGELPLTPNGKIDRKALPAPEAASDANYIGPSGEIEERLTD
                     IWADVLKTDRSLIGANSNFFELGGHSIRALHLINRIQQAFAVQLPLAKIFGHSSIQKL
                     AALIIQLQEEPGRHKTDVIRRAGQRDRYPASAAQERLFYMQLSNKDSVANNIHIAYEI
                     RGSADSYRLVRSLQKLIQRHEGLRTWFSLSGGELFQHIRDEAELPVRIFDPGRYRTPG
                     DVQRELIRPFDLSGCPLMRCGLWQECPTRTYLFVDIHHIVCDGISLNALMNEFKSLYS
                     GEEPALPEFRYVDYAIWEKEQDFGLQREFWDRQLFPGLAGLDLPVIADRGSVEIDRAE
                     SRMLRIEGPLYQKIRAYTAAAEVSDLMFLLSVYSILLAKLSGQSELVIGTDAAGRTLP
                     EWKNIVGTFVNVLPIRLRMPGHLAYRPFLEEVKRTVLEASDHQDYPFDRMNTKVQVYF
                     SFTDFFESEVRLRELEFVSVDAGDERTTRYELELSVGRGADWLDIHFLYSADLYDASI
                     IGLMMRYYQEILATVIDKEDVLLENIHL"
     gene            2494..3606
                     /locus_tag="VGQ51_08150"
     CDS             2494..3606
                     /locus_tag="VGQ51_08150"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEV9036576.1"
                     /translation="MKNYLTKHLYSRMAEAVYPAGIEGLCQLQLVDEGEIFFCNLDCR
                     NGGIGYSEGSVPHSDAVVRMGYDTLAYILSTTDRFDLRNPEILAKVGVEGDIDLVGFL
                     FSLVKRPSERIERLIKETEKNTFGYKDKVREIDRVCRPDRETVMRLMSRSIPFVITGA
                     IDDCPLLWKTLEEIKSEFGEVILRPDMEKGDGKNETFGDFISRMENNRNEQVYTFGCP
                     LPLDLWVQLPLPFFDWEHLTTPQIWMGKKTGDRPCTNLHRDCAHGMLANIFGRKRLVL
                     FSPDQSESLAPIRAFNTFQPCQVKDVHRVDIERFPLFRNAKPLEVTIGAGEILVIPAL
                     WYHCVFALDDVFSVSFGLLWDAWDTMNAPAPALESF"
BASE COUNT          795 a          861 c         1180 g          812 t
ORIGIN      
        1 ggcgggcgaa gacgactgcc tgctggcggt gacgagcacg tccttcgata tatcggtgct
       61 ggagcttttc tggacgctgt gtaacgggat acaggtggtg atccatccct ccggcatctc
      121 cttcagcggt ctggaccgct acctggacca tgaagccggt cgggtacacc gaccggtgac
      181 gatgctgcaa tcgacgccgt cgttcatgcg gatggtccgg gagggggaag ggtctcgtgt
      241 ttttctgcgg tcgctgagga ggctgttatt gggaggggag ggggtgccgt tatcgctggt
      301 gaagggtttg cagcgggata cgggagccgg gatatataat atgtatggac cgacggagac
      361 gacgatctgg tcgtgtatgc atgatttcgg tggggaggag gagaaggtgg tagtgggtgg
      421 tccgatggtc aatacacagg tatatatcct gagcagggag cttcgtcttg ttccggtggg
      481 tgtagccggg gacgtgtata taggagggga gggtctgtcg cgcgggtatt ggcgccggcc
      541 ggagctgacg gcggagcggt tcattgcgca tccgtaccgg tcgggtcagc ggttatacaa
      601 gaccggggat ataggccggt gggtgcgtgg cgggcggatc gagttgctgg ggcgggagga
      661 tcaccaggtg aagatccggg gtcaccggat agagttggga gagatcgagc gggtgttatc
      721 gcagcgggag cagacgggag aggtggtagt ggtggcccgg gagcgggaag gggataagtt
      781 gctgacggcc tattatacgg gagaggccgg cggggaggag ttgaggacgt atctgtcggg
      841 tcggttgccg gagtatatgg taccgtcgta ttttatgaga ctcggcgagc tgccgttgac
      901 gccgaacggg aagatagacc ggaaggcgtt gccggcgccg gaagcggcgt ccgatgcgaa
      961 ctatatcggg ccctcgggag agatcgagga gaggctgacg gatatatggg ccgatgtcct
     1021 gaagaccgac aggtcgctga tcggcgccaa cagcaacttc ttcgagctgg gcggccattc
     1081 cattcgcgcg ctgcacctca tcaacaggat ccaacaggcg tttgccgtac agctgcccct
     1141 ggcaaaaata ttcgggcatt cttccataca gaagctggca gcgctgatca tccaactaca
     1201 ggaggagccc ggccggcata agaccgacgt catacggcga gccggtcagc gcgaccgtta
     1261 ccctgcttcc gccgcccagg aaagactttt ctatatgcag ctgtcgaaca aagacagtgt
     1321 tgccaacaat attcatatag cttacgagat aagagggtcg gcagacagtt accggctggt
     1381 gcggagcttg caaaaattga tacaacggca tgaagggttg aggacctggt tctccctgtc
     1441 gggtggcgaa ttgttccagc atatccggga tgaagcggag ttgccggtac ggatattcga
     1501 tcccggccgc taccgaacgc cgggggatgt gcagcgcgag ctcatccggc cgtttgactt
     1561 atcgggttgc ccgctcatgc gatgcggcct atggcaggag tgcccgacgc ggacctacct
     1621 gttcgtggac atccatcata tcgtgtgcga cggcatttcc ctcaatgccc tgatgaatga
     1681 gttcaaaagc ctttacagcg gagaagaacc agcgctgcct gaattccggt atgtggacta
     1741 tgccatatgg gaaaaagagc aggatttcgg attgcagcgg gaattttggg atagacagct
     1801 gtttcccggg cttgccggat tggatctgcc cgtcatcgcc gaccgcgggt cggtggagat
     1861 cgatcgcgcg gaaagccgta tgctgcgtat agaggggccg ctttaccaaa agatcagggc
     1921 gtataccgca gctgcggaag tatcggatct catgttcctg ctgtctgtct attccatctt
     1981 gctagccaag ctgtccggcc aatcggaatt ggtgatcggc acggatgccg ccggcaggac
     2041 gctaccggaa tggaaaaata tcgtgggtac ttttgtgaat gtactaccga tcaggctacg
     2101 gatgccggga catctcgctt accggccatt cctggaagag gtgaaaagaa cggtcctgga
     2161 agcctccgac caccaggatt atccgttcga ccggatgaat acgaaggtgc aagtctattt
     2221 ttcttttacg gacttttttg aaagcgaggt ccggctccgg gaactggaat tcgtttccgt
     2281 cgatgcggga gatgaaagga ccacacgtta tgagctggaa ctgagcgtcg ggaggggtgc
     2341 cgactggttg gacatccact tcctttacag tgcggatctc tacgacgcat cgatcatcgg
     2401 gttaatgatg cggtattacc aggagatact tgccactgtt atcgacaagg aggacgtcct
     2461 tctcgaaaat atacatcttt aaacttttta atcatgaaga actatctgac gaaacacctg
     2521 tattcccgga tggcggaagc ggtctatcct gccggcatag aaggtttgtg ccagttgcag
     2581 ctggtagacg aaggagagat ctttttttgc aacctcgatt gccggaatgg cgggatcggc
     2641 tattccgaag gcagcgtacc gcattccgat gcagtggtca ggatgggata cgatacgctg
     2701 gcctatatcc tttcgactac ggacaggttt gatctccgca atcccgagat cctggcgaag
     2761 gtgggagtgg agggggacat cgacctggtg ggttttctgt tctcgctggt caaacggcct
     2821 tcggaacgga tcgagcggct gatcaaagag accgagaaga acaccttcgg atacaaggat
     2881 aaggtccggg agatcgacag ggtctgccgg ccggaccggg agacggtcat gaggctgatg
     2941 agtcggtcga tcccttttgt gataacaggg gcaatagacg actgtccgct gttgtggaag
     3001 acgttggaag agatcaaaag cgaattcgga gaggtgatcc ttcgaccgga tatggagaag
     3061 ggcgacggga agaacgagac gttcggagat ttcatttcca ggatggagaa caacaggaat
     3121 gaacaggtat ataccttcgg ctgtccattg ccgctggatc tgtgggtgca gctgccgctg
     3181 ccttttttcg actgggagca cctgaccacc ccgcagatct ggatgggtaa gaaaacaggt
     3241 gacaggccct gcaccaacct gcaccgggat tgcgcacatg gaatgctggc caatatcttc
     3301 ggtaggaaac ggcttgttct tttttcaccc gatcaatccg agtcgcttgc acctatccgt
     3361 gccttcaata ccttccagcc ttgccaggtg aaagacgtgc accgggtgga tatagaaaga
     3421 ttccctttgt tcaggaatgc aaaacccctg gaggtgacta tcggcgcggg agagatattg
     3481 gtgatccccg ccctatggta tcattgcgta ttcgccctgg atgacgtgtt ttccgtcagc
     3541 tttggactgc tatgggacgc ctgggatacg atgaatgctc ctgccccggc cctggaaagc
     3601 ttttaaaacg aattgtatgt gcgggatctg cggatatcta tactttga
//
LOCUS       DASYSV010000266         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1455935, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000266 DASYSV010000000
VERSION     DASYSV010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455935"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..319)
                     /locus_tag="VGQ51_08155"
     CDS             complement(98..319)
                     /locus_tag="VGQ51_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036577.1"
                     /translation="MDQALDQPIELKDPKLKDIAHSWVDSSGFLFYLYVFCVVALVIG
                     GSYGLWAHRYKGKPEVNVPGNTLYTPQYK"
     gene            complement(353..1381)
                     /locus_tag="VGQ51_08160"
     CDS             complement(353..1381)
                     /locus_tag="VGQ51_08160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9036578.1"
                     /translation="MMPPTTAIVILNFNGRRYLEQFLPFVVANTCPGAEVIVADNGSS
                     DDSLVFLAAAYPGVRVIVLDRNYGFAGGYNTALGQVDADYYVLLNSDVEVGPGWMDPI
                     IRLLESDASIGACQPKIRMYADRESFEYAGAAGGWLDHLGYPFARGRVFDVCEKDQGQ
                     YDKTEPVFWASGAALFIRARLYHQLGGLEGFFFAHQEEIDLCWRMQLAGYQVYCCPES
                     VVYHVGGGTLPKGNERKVFLNFRNNLIMMAKCLPFRQWIWKLPLRFLLDSISAWKSLF
                     AGEAIYFWAILEAHLAFFKWLMVSRKKSLFPPKKRAPLAGWYTGSVVWQYFVRGKKTF
                     TEIVRRNR"
     gene            1456..2700
                     /locus_tag="VGQ51_08165"
     CDS             1456..2700
                     /locus_tag="VGQ51_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV9036579.1"
                     /translation="MIRSMLNWRSLLALVAIGIVTATIFYSRYLARKIAADERQKVTL
                     WVAASKSILSNPGMDLTLPNLIRNGQQTIPIIETNENDSIMEYINLDSIRAARDRDWL
                     YRELKKFRAENQPLEIKLSDTPYIANRYYYGHTSLLDEVRYYPLVQLFIVALFIFFIL
                     YSITIRNKATQNQLWAGMAKETAHQLGTPVSSLEGWIEMLREGGTDPRTVQEIQKDVD
                     RLKLVSDRFGKIGSIPHLEERDLIAQIRYMMEYIRKRATGKVQFALETPGRNGDAAIM
                     PPGSLASMPPGSPASPPATAAMYVSAPLMARISGPLFDWVIENLLKNALDAIEGKGSI
                     TITLSEHDKEILIDVTDTGKGIAARNVAKVFKPGFTTKKRGWGLGLSLSKRIIEQYHK
                     GQLFVRQSEPGKGTTFRIVLKK"
BASE COUNT          722 a          812 c          685 g          593 t
ORIGIN      
        1 ggttgcgctc taccaactga gctacttccg cgcgtatgat tccttaccgg gggtgcaaaa
       61 atagtaaaac tatgattccc agacaaaaat gtttgcctta tttgtattgc ggcgtataca
      121 gcgtattacc gggtacattc acctcgggtt tgcccttata acgatgggcc cacagaccat
      181 aagagccgcc aatgaccaat gcgacgacac aaaacacata caggtaaaaa agaaagccgg
      241 aagagtcgac ccaactgtgc gcaatatctt tcagtttcgg gtcttttagc tcaatgggtt
      301 gatctaatgc ttgatccata atgatttaat tgctgccgca aatttaagaa attcaacgat
      361 tcctccttac aatttcggta aaagttttct tcccccggac aaagtattgc cagactacgc
      421 tgccggtata ccatcctgcc agcggagccc gctttttggg agggaaaagg ctttttttgc
      481 gactgaccat cagccatttg aaaaaggcca ggtgggcctc caaaatagcc cagaaataga
      541 tggcttcacc ggcgaacagc gatttccagg ccgagatgga gtccagcagg aaacgcagcg
      601 gcagcttcca tatccattgt ctgaaaggca gacacttggc catcatgatc aggttgttcc
      661 ggaaattgag aaagaccttt cgttcattac ccttgggaag cgttcccccg ccgacatgat
      721 agaccaccga ttcgggacag caatagacct gataacccgc cagctgcatc cgccagcaca
      781 gatcgatctc ttcctggtgc gcaaagaaaa acccctcgag gccacccaac tgatgataaa
      841 gacgcgcacg gataaaaagc gccgcaccac tggcccagaa aacgggttcg gttttgtcat
      901 actgcccctg atccttttca caaacgtcga atacccgtcc ccgcgcgaag ggatagccga
      961 gatgatccag ccacccgccg gccgcgccgg catattcgaa tgattcgcgg tcggcataca
     1021 tccgtatctt gggttgacat gcgccgatcg aagcatcgct ttccagcagc cggatgatcg
     1081 ggtccatcca gccgggtccc acttccacat ccgagttcaa aagaacatag tagtcggcat
     1141 ccacctgtcc cagcgcggta ttgtatcctc ccgcaaaacc ataattgcga tccagcacga
     1201 tgacgcgcac accgggatat gccgcagcga gaaagacgag tgagtcatcc gaagagccat
     1261 tgtcggcaac gatgacctcc gcccccggac aggtattggc tactacgaaa ggaaggaatt
     1321 gctcgagata tcggcgtccg ttgaagttga gtatgacgat ggcggtcgtg gggggcatca
     1381 ttcggtataa agttcaaaaa taagcttccc ccggcttttc gcccattact ggcccggatt
     1441 ttatacattc ggttcatgat tcggtcaatg cttaactgga gatcgctgct ggcgctggtc
     1501 gccatcggca tcgtgacagc gaccattttt tattcccgtt acctggcaag aaagattgcc
     1561 gcggacgagc gacaaaaggt cacactgtgg gtagccgcca gcaaatccat tctctccaac
     1621 cccggcatgg acctgaccct tcccaatctc atccgcaacg gtcagcagac cattcccatc
     1681 atcgagacca atgagaatga cagtatcatg gagtatatca acctcgactc cattcgcgcc
     1741 gcgcgcgaca gggactggtt ataccgcgaa ttgaaaaagt tcagggccga gaaccaaccc
     1801 ctcgaaataa agctgagtga cacaccctat atcgcaaacc gttattatta cggtcatacc
     1861 tccctgctcg acgaggtccg ttactatccg ctggtgcagc tgttcatcgt ggccttgttc
     1921 atcttcttca tcttatattc gataaccatc cggaacaaag ccacgcaaaa ccagttgtgg
     1981 gccggtatgg ccaaagaaac ggcccaccag ttgggaacac cggtctcctc gctggaaggc
     2041 tggatcgaga tgctccgtga gggcggcacc gacccacgga ccgtgcagga gatccaaaag
     2101 gatgtcgaca ggctgaagct ggtctccgac cggtttggca agatcggcag catcccgcac
     2161 ctggaagaac gcgatctgat cgcgcagatc aggtatatga tggaatatat ccggaaacga
     2221 gccacgggaa aggtccagtt cgcgctggaa actcccggcc gcaacggcga tgcggccatc
     2281 atgccccccg gcagtctggc ctccatgccc cccggcagcc cggcttcccc tccggcaacc
     2341 gcggccatgt acgtatccgc tcccctcatg gcgcgtatct caggcccgct gttcgactgg
     2401 gtcatcgaaa atctgctaaa gaatgcgctc gatgccatcg aaggaaaagg ctcgataacg
     2461 atcacccttt ccgaacacga caaagagatc ctcatcgatg ttaccgatac gggcaagggc
     2521 atcgccgccc gcaatgtcgc caaggtcttc aaacccggat tcaccaccaa aaaacgtggc
     2581 tggggcttag gcctgagcct ctccaaacgt atcatagaac aataccataa aggccagctt
     2641 ttcgtccgcc agtccgaacc ggggaaagga actactttca ggatcgtgct gaaaaaatag
     2701 gcgccaccgg accgcaaaat cccacccgaa agtcctattt ttgcagccac ctcaaaaatt
     2761 ttgacacttc tcatctgcgt gggtggcgaa attggtagac gcactacttt ga
//
LOCUS       DASYSV010000267         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2095681, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000267 DASYSV010000000
VERSION     DASYSV010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2095681"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VGQ51_08170"
     CDS             complement(<1..606)
                     /locus_tag="VGQ51_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEV9036580.1"
                     /translation="MGLVVAAIGTTPWAFLVGLNFKYASAVPWAVPVTAVYLWFYWRY
                     FTGAWGPPRSNLEARKKLCRANALSDEVWGAALLAGVAGLAFIVVSQTVLDRMIRLPM
                     QATDELPHVPVLMLFFSIIMGAIVAGVAEESAFRGYMQGPLERRHGPVVAILTVGVLF
                     GFAHFSHPEVGFAMMPYYIIVATVYGMLAYFTNSILPSLVLH"
     gene            complement(660..2078)
                     /locus_tag="VGQ51_08175"
     CDS             complement(660..2078)
                     /locus_tag="VGQ51_08175"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEV9036581.1"
                     /translation="MGSFFTLTGRLHPLRVHLPIGILLLAIMLEILSGRARHQGLKQA
                     ADLSLLIGFLCAVLSCITGLLLSQSGDYDESVVNVHQWLAISLTIVSGMLYVTLHGKP
                     MTRQGGALTAGVVVLLILTGHWGGTLTHGPGYLTANLEPAVSAPALRPIADVQAAGVY
                     IDLVQPVLHDNCYGCHRMGRVKGGLRLDIPDGIMKGGKDGPVVVAGDAAASLLLKRVL
                     LPLDDDHHMAPKDKNQLTIREIQLLKWWVDNGAPFDKTVASLRQDTAAAAMLLAFHNG
                     TAAPVAASMVTNIADSDLPMGQARPAPAEAVKVLRAAGAVVLPIDQASNYLSVDLAGK
                     PVSSAVLRAIPMLREQLVSLKCSWSKVGDEVVSAAAACPLLVRLWLDHTAITGANLDT
                     LCGLGNLKYLNLTGDAVGAAQVSGLKGAPKLAEIYLYQTKVGRGDWDVLRRDFPQAIL
                     DSGGYGIPFLASDTAIVREPAK"
     gene            complement(2078..2995)
                     /locus_tag="VGQ51_08180"
     CDS             complement(2078..2995)
                     /locus_tag="VGQ51_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV9036582.1"
                     /translation="MINRKQFIAQTTMAATAVLFESYAHPLVSPSVRDGFQLKILATD
                     WGFPGNLDEYGAKVKAAGYDGIEIWWSLESARQDEVFSMLRKYGLEVGFLCAGSEPEV
                     AAHFEHFKKMVEGAARNTRQRPLYINCHSGHDFFSYEENSRFIDLTTALSRETGIRIL
                     HETHRSRMLFAAPVARHYLEAHPDLRITFDVSHWCNVSESLLADQPKTMALALERADH
                     IHARIGHPEGPQVNDPRAPEWQPAAEAHFAWWDAILRRKKQQGEVMTILTEFGPPDYM
                     PTMPYTHKPLADQWAINEYMLHTLRTRYA"
     gene            complement(2992..>3476)
                     /locus_tag="VGQ51_08185"
     CDS             complement(2992..>3476)
                     /locus_tag="VGQ51_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEV9036583.1"
                     /translation="RFVQIFDWGWDSHGTDASLAIDVGFINKCRQVDRAMTALLLDLK
                     QRGLLDETLVVWGGEFGRTPMMENREGKPNPFKGRDHHTDAFTIWMAGGGIRKGHSHG
                     ETDEIGYTGISGKVDTFDVQATILNQLGFDHTRFTYDFQGRPFRLTDVKGNVIKEIIA
                     "
BASE COUNT          807 a         1118 c          864 g          687 t
ORIGIN      
        1 atgcaaaacc aggcttggca agatcgaatt cgtaaaataa gccagcatgc catacaccgt
       61 ggccactata atataatatg gcatcatcgc aaacccgact tccggatgac tgaaatgcgc
      121 aaatccaaac agcaccccta cggtcaggat cgcaaccact ggcccatgcc gccgttccag
      181 cggcccttgc atatatcccc tgaaagccga ctcctccgcc actcccgcaa cgatggctcc
      241 cataatgatg gaaaagaaca gcatcagcac cggcacgtga ggtagctcat cagtagcttg
      301 cattggcaag cgtatcatgc ggtcgagcac ggtctgagac actacgataa atgcgagtcc
      361 cgctacccct gccagtaggg cagcgcccca cacctcatca gaaagagcat tcgcccggca
      421 gagtttcttt cgggcttcca ggttcgatcg gggtggcccc cacgcacctg taaaatatcg
      481 ccagtagaac caaagataca cagccgttac cggcaccgcc cagggtaccg cagaagcata
      541 tttgaagtta agccccacga gaaaagccca tggcgttgtt ccgatggcgg caacaacaag
      601 acccatgagc ggagcgctga taataacagg taccctttgc catatcgata ttatcttatt
      661 cacttcgccg gctcccgtac tatcgcggta tccgaagcca ggaacggtat cccatacccg
      721 cccgaatcca atatcgcctg cggaaaatcc cgccgcaaga catcccaatc gccccgaccc
      781 accttcgtct gatacaaata gatctccgcc agcttaggcg cccctttcaa ccccgaaacc
      841 tgcgccgccc ccaccgcatc acctgtcaga ttcaaatatt tcaaattacc cagtccacac
      901 aacgtatcga ggttcgcccc cgtgatcgcc gtatgatcca gccataaccg cacgagcaga
      961 ggacaggccg ctgcagcgct caccacttca tcccccacct tcgaccagct gcatttaaga
     1021 gaaaccaact gctcgcgaag catcgggatt gcgcgcaaca ccgcagaact caccggcttg
     1081 cctgccagat ccaccgaaag ataattgctg gcctgatcga tcggcaacac caccgccccc
     1141 gctgccctca gcaccttcac cgcctccgcg ggcgcagggc gagcctgtcc catcggcaga
     1201 tcgctgtccg caatattggt caccatcgag gcggctaccg gcgccgccgt accgttgtgg
     1261 aaggccaaaa gcatcgccgc cgctgccgta tcctggcgca gcgacgccac ggtcttatca
     1321 aaaggcgcgc cgttgtcgac ccaccacttc agcaactgga tctcccggat cgtcagctga
     1381 ttcttatcct tcggcgccat atgatgatcg tcatccaatg gtagcaggac tcgcttcaaa
     1441 agcaaactcg ccgcagcgtc accagcaacc accacaggtc catcctttcc gcctttcatg
     1501 atcccgtcgg gtatatccag ccgcaggcct cccttgaccc ggcccatccg atgacagcca
     1561 taacaattgt catgcaacac cggctggacg agatcgatat aaacccctgc cgcctgcaca
     1621 tcggctatcg ggcgtaatgc aggtgccgac accgccggct ccaggttcgc agtcagataa
     1681 ccgggaccgt gagtaagcgt cccgccccaa tgtcccgtca ggatcaacaa aaccaccacc
     1741 ccagcagtga gcgcaccccc ctgccgcgtc atcggcttgc cgtgcaaggt cacatacaac
     1801 atacccgaaa cgatcgtcag cgaaatagcc agccattgat ggacattgac caccgattca
     1861 tcatagtcgc cggattgaga caggagcaga cccgtaatac aggacaacac cgcgcaaagg
     1921 aacccgatca gcaggctgag atcggcggcc tgctttaacc cctggtgccg ggcgcgcccg
     1981 ctcaatatct ccagcatgat cgccagcagc aggatgccta tgggaagatg aacgcgaagg
     2041 gggtgcagcc ggccggtcag ggtgaaaaag gagcccatta tgcgtatcgt gttctcaggg
     2101 tgtgaagcat gtattcattg atcgcccatt gatccgcgag cggtttgtgc gtatagggca
     2161 tcgtcggcat ataatccggg gggccgaatt ccgtaaggat cgtcatcact tcaccctgtt
     2221 gtttcttgcg gcgaaggatc gcatcccacc atgcgaaatg cgcttcggcg gccggctgcc
     2281 attccggcgc cctcgggtcg ttgacctgtg gtccttcagg gtgcccgatc cgggcgtgga
     2341 tatgatctgc ccgttccagc gccagcgcca tcgttttcgg ctggtcggcg agaagacttt
     2401 cactgacatt gcaccagtga ctaacgtcaa aggttatccg cagatcgggg tgcgcttcca
     2461 ggtagtgacg cgcgactggt gcggcaaaca acatcctgct ccggtgggtc tcatgcagga
     2521 tccggatgcc tgtctcgcgg ctaagcgccg tcgtaagatc gataaaccgc gaattttcct
     2581 cataggaaaa aaaatcatgc cccgagtggc agttgatata taaaggtcgc tgccgcgtat
     2641 tccgcgccgc accctccacc attttcttaa aatgttcgaa atgtgcagcc acctcgggct
     2701 ccgaacccgc gcaaagaaat cctacttcga gcccgtattt ccgcagcatg gaaaagactt
     2761 catcctgccg cgccgattcc agcgaccacc agatctcgat gccgtcataa cctgcggcct
     2821 tcactttggc gccatactcg tccagattac ccgggaaacc ccagtcggtg gcaaggatct
     2881 tcagctggaa gccatcccgg accgaaggcg acaccagcgg atgtgcataa gattcaaata
     2941 atacagcggt agcggccatg gttgtttgtg cgatgaactg ttttcggttg atcatgctat
     3001 aatctctttg atgacgtttc cctttacgtc agtaagccgg aaaggccggc cctgaaaatc
     3061 ataggtaaag cgcgtatggt cgaatccaag ctggttcaaa atggttgcct gcacgtcgaa
     3121 ggtatcaact ttacccgata tgccggtata cccgatctca tcggtctcgc catggctatg
     3181 tcccttgcgg atgccgccgc cggccatcca gatcgtaaag gcgtcggtat ggtgatccct
     3241 gcccttgaag ggattgggct tgccttcgcg gttctccatc atgggcgtcc ggccgaactc
     3301 accgccccag acgaccagcg tctcgtccag caagccgcgt tgtttcagat ccagcagcag
     3361 cgcagtcatc gccctgtcca cctggcggca cttattgata aagcccacgt cgatggccag
     3421 actggcatcg gtaccatggc tgtcccagcc ccagtcgaat atctgcacga accgca
//
LOCUS       DASYSV010000268        13341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_946830, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000268 DASYSV010000000
VERSION     DASYSV010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13341
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946830"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..3415)
                     /locus_tag="VGQ51_08190"
     CDS             complement(116..3415)
                     /locus_tag="VGQ51_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9036584.1"
                     /translation="MSKRITRSVIALSVVFLLPLASIAQKLITGHVLSNVDQSPVPGV
                     SVVIKGTRIGTSTAVDGSFALRAKEDDMLVISGIGVKSTEQPVTGADITINVVANARE
                     LNQVVVTATGIKKEAKRLGYAIQTIDAATLTTAREPDPVNSLRGNAAGLEVNINQEIG
                     HSPDVIIRGENDPNDRPIFVIDGVPVNSDTYNINPDDIETFTILKGPNAAALYGFQGR
                     NGAIIISTKKGAKAKGKMLVSVNSSTQMNKGFIALPKYNDEYGPGDNGKYAYGGGGSS
                     PASYFGSGAVGVGINDYDYDVWGPQFRGQLIPQYDGQYTANQSYTTTFADGSAYIGHV
                     APTPFIARGKDNLKKFIQTGLLSSNSIAVSSSTDNTDMRISAGNIYQQGIVPNTQLNN
                     ANFTTNVTHRFSDKLSLTTYFNFSRQSTPNVPDVTYGPNSIIYNIIIWGGADWSMADM
                     RDYWQPGKVGIQQKYAEYYRYNNPWFMSYEWLRGHYQNNEYGYLSLNIKVTPSLDIQF
                     RPSLSAYDMTNSEKLPYSADVYGRELRQGDYREDRRAMFESIEDLQVRYHRNEIAGFL
                     DLQALGGASVRSFNFNSDFESTNYLNVPGIYAFSNSQGTLTGSSFHSSMLVLSTYGSV
                     DLGYKSYINANITGRVDKSSTLPTNVNSYFYPSVNLSTVISEYVHLPEAISFLKVRAS
                     YAATRSGGTYAFFSPNVSSTPAAGYGYTWNSPYNGPSYQFSQPYRLSPTYSSQSSAVY
                     TTQTVDPNINTADRKATEVGLDIRFLRNRLGLDLTRYHYKNKSIVNQLTSNASGYSSY
                     LTNGDVYTNDGWEATLSGTPVSSPDGFIWNVVANFSTYEKKWVNYVSNPNNYEKNGAR
                     VDLVYSDAFVRTPDGKMVIDPGSGVYLRYSDLGVSANKIYGHADPDWQWGVTNTFTYK
                     SFAFRFQFDGLVGGVLEDYVRKKTLQGGRHIESATGALGAARPSDEANVAAYTGQGVL
                     LTGAPIQLDPINGKILNMKDLTETSNNVKSQVQPFVTREASIPDLDIVKKTNAKLREV
                     TITYKIPQSTFGKKSIISNASVSLVGRNLLYFFPDKYKDLDVDQYTQDSGSGLQTPTM
                     RSYGFNINFSF"
     gene            3530..3796
                     /locus_tag="VGQ51_08195"
     CDS             3530..3796
                     /locus_tag="VGQ51_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036585.1"
                     /translation="MRAAPKQIKHQHQGLAGADIPETMSCAAVEEERIAFVQSEYFFF
                     HLVFDIAFENVFAFDEDVIVAEAVVHVFEERSEILLQNDLVEGL"
     gene            complement(3807..4571)
                     /locus_tag="VGQ51_08200"
     CDS             complement(3807..4571)
                     /locus_tag="VGQ51_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEV9036586.1"
                     /translation="MRNIFLFLLFVALRASAQPVRYTVANTHSHNDYEQPIPFWSAWQ
                     ARFGSIEADIWLVDGRVIVGHDRQEVSAGRTLEEYYIRPLEACMEKNGGHPYADTGRR
                     LQILIDVKADSVATLDALISLLNKYPLLEHSRSLTWVISGNRPAPDRYTYYPAFIGFD
                     GVLHTQYSAAAFSRITLMSDDLEEYTKWNGLTDMPSADRGRLEDAIRDAHRHHLPVRF
                     WDAPDFSRAWQSLQELHVDYINTDHIDELAEWLNSR"
     gene            4792..5631
                     /locus_tag="VGQ51_08205"
     CDS             4792..5631
                     /locus_tag="VGQ51_08205"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEV9036587.1"
                     /translation="MEKRPVDVVVISDVHLGTYGCRAKELVNYLKSITPNILILNGDI
                     IDGWQFTKRYFPVAHMMVIKEIIQFITSGTRVFYITGNHDEMMRRYTDIQMGNFTLTD
                     KLVLEIDNKMTWIFHGDVFDNTTKGGARILAKLGSSGYNLLIRFNRFINFILKALGRE
                     RVSISKKVMAGVNKAVAKINDFEAIAAELAIGKKYDYVICGHIHQPQKKIVETKDGKV
                     TYLNSGDWVEHLTALEYQQNQWSIFEYDEKNFPVTQTVEMKPSVSVLTDEISFYINSL
                     AAI"
     gene            5637..6674
                     /locus_tag="VGQ51_08210"
     CDS             5637..6674
                     /locus_tag="VGQ51_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HEV9036588.1"
                     /translation="MKIFYAIQATGNGHISRAMEMIPYLDRYGKVDIFLSGANNTLEL
                     DAPVKYRSKGLSLFYTHSGGLNYCEIVRKVSPLRLWREVRDLPVEKYDLVINDFECIT
                     ALACAHKRVPSVQFGHQASFVSPKVPRPEHRERMGEWILRNYARATQYIGLHFRQYDD
                     FILPPVIKKEILQADPEDNGHITVYLSSYSDATVKRYLQPLKEFSFQVFSKEVHTPAR
                     DGNILFIPVSKGAFNRSLITCKAILTGAGFETPAEALYLGKKLMVIPIRGQYEQFCNA
                     AALSKMGVPVLHALDAGFDGAFRGWMAQKRRPHLNLDYSTESIVSYLMHHCGRGKADA
                     LDMLYPDCVFN"
     gene            complement(6932..7597)
                     /gene="phoU"
                     /locus_tag="VGQ51_08215"
     CDS             complement(6932..7597)
                     /gene="phoU"
                     /locus_tag="VGQ51_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV9036589.1"
                     /translation="MAQIETELQNLKTEAINMWTLVNSQLSKAQQAFLNFDKDLAREV
                     VLKEKRVNGNELKIDRDCENIFALFCPVAVDLRFLLAILKINNNLERIGDYAEGIAKY
                     VIDAEAPFSDQMLKITNILPMYEEAVNMLEDTQVAFEKEDTILARRVFKKDDLLDDVN
                     KKSHHILEVHIMEHPEDASQALWLLSIIRRLERVGDQTKNIAEEIIFYLEAKVLKHRN
                     KFV"
     gene            complement(7670..8428)
                     /gene="pstB"
                     /locus_tag="VGQ51_08220"
     CDS             complement(7670..8428)
                     /gene="pstB"
                     /locus_tag="VGQ51_08220"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEV9036590.1"
                     /translation="MESSVIKSRNFNASFGANHVVKNVNIDIPRNHIIAVMGPSGCGK
                     TTYLRCINRMHELIPGANSSGEMLLNEEDVYKMHPIFVRLKIGMVFQRPNPFPNLSIY
                     DNVIAGYKLNGVKLPKAEKDRIVEHSLTRVALWNEVKDSLTKKGSFLSGGQQQRLCIA
                     RAVAMEPEVILFDEPTSALDPISTSTVEELFHELKANYTLIVVTHNMQQAARVSDKTA
                     FFFLGELVEYDETKTMFTNPKDNRTQNYITGRFS"
     gene            complement(8410..9258)
                     /gene="pstA"
                     /locus_tag="VGQ51_08225"
     CDS             complement(8410..9258)
                     /gene="pstA"
                     /locus_tag="VGQ51_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV9036591.1"
                     /translation="MQAKSVKYRLGKSYGFQILTYILAFAIVVPLLAILFYIFRAGIT
                     RINWHFLVNIPKPVGETGGGIANAIVGSIIIILIAAVIAVPIGIMAGVYLSENRKSRL
                     AYWVRLSTDVLQGVPSIVIGIIAYFWLVKPLRGFSALSGSVALAIMMLPIIVRSTEET
                     LKLLPDSLKEAALALGVPFHRVMFKVIVPVGISGILSGVMLAIARITGETAPLLFTAF
                     GNPYLSVNIAKPMQSLPLLIFNYATSPYTDWLNLAWGASLILLVWVLFLNILTKLLTR
                     KWKVQL"
     gene            complement(9352..10314)
                     /gene="pstC"
                     /locus_tag="VGQ51_08230"
     CDS             complement(9352..10314)
                     /gene="pstC"
                     /locus_tag="VGQ51_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV9036592.1"
                     /translation="MITRSDLPLRVINSSPEAIAKAKKRIARSFKQIRWENIFNKTLM
                     IMGLIMIILVLGILFTLIVQSLPSIKALGLKYLWGKTWNPVEDIYGAWPFLLGTLLTS
                     FLALIISIPFSIAVAVFLGEYHTKGWVPNFLKNAVELIAAVPSVIYGFWGLAVLVPMV
                     RSFEGHVGADPLGVGIFSSSLILAIMIIPYAASLGRSMITLVPSHLKEAAYGLGATRW
                     EVIRSVILPYTKSGLFAGILLSLGRALGETMAVTMVIGNSSAIPKSIFAPGNTMASVI
                     ANEFTEADKPVYLSALIELGLVLFLVTVVINLIGKRVIDRFTND"
     gene            complement(10355..11464)
                     /gene="pstS"
                     /locus_tag="VGQ51_08235"
     CDS             complement(10355..11464)
                     /gene="pstS"
                     /locus_tag="VGQ51_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV9036593.1"
                     /translation="MKKNIFSIKNALLAGASFAILTVSLISCGGGTSGGSDSTASASG
                     DDNTLIGAGSSFDNPLFSKQFSEYNKTNGLKINYQSVGSGAGIAQLTAKTVDFGASDA
                     PMNGKQDSAVSGPVVHIPITAGAVAIAYNLPDVKDTLQFTADVLANIFLGKITKWNDP
                     KIAAINKGVKLPSTGIVVAHRSDGSGTTNIFTTYLSKISEDWNKTVGKGSSVNWPTGL
                     GGKGSEGVGGLVKQTPGAVGYIELAYAVQNNMPYARIQNKSGNYISPSIPAVSAAADI
                     QIPADTKVSLTNTDAANGYPISGFSWVLLYKEQKYGDRSADRAARLVKLIDWMIRDGQ
                     QYSSALTYAPLSKAAVDAGEAQLKTVTYDGKPILQ"
     gene            complement(11505..12824)
                     /locus_tag="VGQ51_08240"
     CDS             complement(11505..12824)
                     /locus_tag="VGQ51_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036594.1"
                     /translation="MKRNFYQQFLLIALLFCLAITGQAQYLFNSGQGTSLWNSDSAFK
                     AGVPNSGRIWGYAFGDYFHKAHSDSLNRGGNNQYSGIKQDIDQFQFRRIYLGYDYNIS
                     KKFSAELLLAAEDDFIAPTQGTGDLAADNKFTFYVKFANIRWHDFLWKGNDLVFGLQP
                     TPSFPYTSENIFSYSRPVERTITDVRRTPSFDFGVGLQGRYQSDDKSTTYGYDFLVAN
                     GSSAKPQALNTEVYKWWYGDAFVAVANRRLFFDIYQDYQRQNWLPTTYGHAYRAMTKG
                     IIAWVDKKFTIGAEGYINTLGNSEVGTRAGGKDTLSGNATGLSLYGHVNLVGTRLRFW
                     ARYDFYNPNTKYDNTTYTRYASLYGVSTSYEPNNKERFLSTGIDYSPVANIHIMPNIW
                     YDRYIGQQANLTGRAARDYDLVYRVTFYFTFGKLFANPGYSYYPYLH"
     gene            13056..>13341
                     /locus_tag="VGQ51_08245"
     CDS             13056..>13341
                     /locus_tag="VGQ51_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9036595.1"
                     /translation="MRLSRPFFLIIVCTVAALLIRPRRSLAQKRTLSIGDVLTLVESG
                     QPQLQAYKEQAAAAGYNVNLARNTLVPNLTGGYQAGYATDNNIAGLSYPGL"
BASE COUNT         3086 a         3214 c         3811 g         3230 t
ORIGIN      
        1 ggttgttggg atcggtttcc ttttgctgga tctgcttgtt gcaggaggta accagcatgc
       61 cgcccataag gatgaaaaat aaaaatttgc gtgtcatatt tttttcttta tggaattaga
      121 aggagaaatt tatgttgaac ccatagctcc gcatcgtcgg cgtttgcaag cccgaccccg
      181 agtcctgagt gtactggtcc acatccaggt ctttgtattt gtcggggaag aaatagagga
      241 ggttcctacc caccagggat acgcttgcat tgctgatgat cgactttttt ccaaacgtac
      301 tttggggtat cttataagta atggtcacct ctctcagttt cgcattggtc tttttgacga
      361 tgtccaggtc cgggattgaa gcttcgcggg tcacgaaggg ctgcacctgg ctcttgacat
      421 tattcgatgt ctcggtaagg tccttcatat tcaggatctt tccattgatg gggtccagct
      481 ggataggcgc gccggtgagc aggacgccct gacccgtata cgctgcgacg ttggcttcgt
      541 cggaaggccg ggcggcgccc agtgcgccag tggcgctttc gatgtggcgc ccaccctgca
      601 gggtcttctt ccggacatag tcctccaaca ccccaccgac gagaccgtcg aactggaaac
      661 ggaaggcaaa actcttatag gtaaaagtat tggtcacacc ccattgccaa tccggatccg
      721 catgaccata aattttattg gcggatacgc ccagatcgga atagcgcagg tatacgcccg
      781 atccggggtc gatcaccatc ttgccgtcgg gtgtcctgac gaacgcatcg ctatatacga
      841 ggtctacccg cgccccgttc ttctcgtaat tattgggatt gctgacgtag ttaacccatt
      901 tcttctcata ggtggagaag ttggctacca cgttccagat gaacccgtcc gggctggaga
      961 ccggcgtgcc gctgagcgtt gcttcccatc cgtcgttcgt atacacgtcg ccgttggtca
     1021 ggtaggaaga ataaccggaa gcattggagg tcagctggtt gacgatactt ttgttcttat
     1081 agtgatacct ggtcaggtcc agacccagcc tgttcctcag gaatcggata tcgagaccta
     1141 cttcggttgc tttacggtcg gcggtgttga tattcgggtc gacggtctgc gtcgtataga
     1201 cagcgctgct ctgactggaa taagtgggtg acaaacggta gggctgggag aattggtagc
     1261 tgggcccgtt gtacggcgag ttccaggtat agccataccc tgccgccggg gtcgagctca
     1321 cgtttggaga gaagaaggca tatgtcccgc cgctcctggt ggcggcatag gaggccctga
     1381 ccttcaggaa ggagatcgct tccgggaggt gtacatattc ggagataacc gtggacaggt
     1441 tgacggacgg atagaaatag ctatttacat tggtcggcag ggtcgaagac ttatccacgc
     1501 ggccggtgat attggcattg atataggatt tatatcccag atcgaccgac ccataggtgc
     1561 tgagcaccag catcgatgag tggaagctgc tgccggtgag cgttccctgt gagttggaga
     1621 aagcatagat cccgggcacg ttcagataat tggtggattc gaagtccgag ttgaagttga
     1681 agctcctgac cgatgcgccg cccagtgcct gcaggtccag gaatccggcg atctcgttgc
     1741 ggtgatagcg aacctgcaga tcctcgatcg actcgaacat cgccctccgg tcctcgcggt
     1801 agtcgccctg gcgaagctcg cgaccgtaca cgtccgccga atatggcagc ttttccgaat
     1861 tggtcatgtc ataggccgaa agactgggcc tgaactggat atcgagactg ggcgtcacct
     1921 tgatgttcag cgacaggtac ccatattcgt tgttctggta atgaccccgc agccattcgt
     1981 acgacatgaa ccaggggttg ttatagcggt agtattccgc atatttctgc tggatgccta
     2041 ccttgcccgg ctgccagtaa tccctcatat ccgccatgga ccagtccgca ccaccccaga
     2101 tgatgatatt gtagataatg ctgttgggcc cgtaagtgac atccggtaca ttcggcgtcg
     2161 actggcggct gaagttgaaa taggtggtga ggctcaattt gtcggagaac cgatgcgtga
     2221 catttgtcgt gaaattcgca ttgttaagct gcgtgttcgg gacgatcccc tgctgataga
     2281 tatttccggc tgaaatgcgc atatccgtat tgtcggtcga gctgctgacg gctatgctgt
     2341 tggaagaaag cagaccggtc tggatgaact ttttcagatt gtccttgccg cgggcgataa
     2401 agggcgtagg cgctacgtgg ccgatatagg cgctgccgtc ggcgaaagtc gtggtatagg
     2461 actggttggc cgtgtattgg ccgtcatact gcgggatcag ctggccccta aattggggac
     2521 cccagacgtc gtaatcgtag tcattgatgc caacgccgac cgcgccggac ccgaaatagg
     2581 aagctgggct cgaaccaccg ccgccatagg catatttgcc gttgtcgccg gggccatatt
     2641 cgtcattata tttcggcagc gcgatgaacc ccttgttcat ctgtgtgctt gaattgacgg
     2701 agaccagcat tttgcctttc gctttggcgc cttttttcgt ggaaatgatg atcgcgccgt
     2761 tccgtccctg gaaaccgtag agagcggcgg cattcggacc tttcaggatg gtaaacgtct
     2821 cgatatcgtc cgggttgata ttataggtgt cggaattgac cggcaccccg tcgatcacaa
     2881 agatcggccg gtcgttggga tcgttctcgc cacggatgat cacatcggga gaatgcccga
     2941 tctcctggtt gatattgacc tccaggcccg ccgcgttgcc tctcagtgaa ttgacgggat
     3001 ccggttccct cgccgtcgtg agggtggccg catcgatggt ctggatagca taccccaatc
     3061 gtttggcctc cttttttatg ccggtggccg ttactaccac ctggttcagt tccctggcgt
     3121 tcgccacgac attgatggtg atatcggcgc ctgtcaccgg ctgctccgtc gatttcactc
     3181 cgatgcccga tataacgagc atatcatctt ctttcgcccg gagggcaaag ctgccgtcta
     3241 cggcggtaga cgtgcctatc cgggtgcctt tgatgacgac ggaaacgccc gggacgggcg
     3301 attgatcgac gttggagaga acatgtcccg tgatgagttt ctgtgcgata gaagccagtg
     3361 gaagaaggaa taccactgac aaagcaatca cgctacgggt aattcgctta ctcataatac
     3421 agtctgtttt taggtgaaag tgaaagggat tcttattccc gaaagtagaa gggtaatgtg
     3481 actggaaggt taggcctgat ttaagtaaat gttaagagtt tacaaataat tgcgggcagc
     3541 gcctaaacag ataaaacacc agcatcaggg cctcgccggg gccgatattc cggagacgat
     3601 gtcctgcgcg gccgtcgaag aagagcgtat cgccttcgtg caaagtgaat acttcttttt
     3661 ccacctggta ttcgatatcg cctttgaaaa tgtatttgca ttcgatgagg acgtgattgt
     3721 cgccgaagct gtcgtgcatg tcttcgaaga acgttcggag atcctcctcc agaatgattt
     3781 ggttgagggg ctataagata ggaagattag cgcgaattca accactccgc cagctcgtcg
     3841 atatgatccg tgttgatata atcgacgtgc agctcctgca ggctctgcca tgcgcgcgag
     3901 aaatccggcg catcccagaa acggacgggc aggtggtgac gatgcgcgtc gcggatggcg
     3961 tcttcgaggc gtccgcggtc tgcagaaggc atgtcggtca gaccgttcca tttcgtatat
     4021 tcttcgagat cgtcgctcat cagggtgatg cgcgagaagg ctgccgccga atactgcgta
     4081 tgaaggacgc cgtcgaagcc tatgaaggcc ggatagtagg tataccggtc gggcgcgggc
     4141 cggttgccgc tgatgaccca ggtcagcgaa cgcgaatgtt ccagcagcgg gtacttattc
     4201 agtaaggaga tgagggcatc gagcgtggcc acggaatcgg cctttacgtc gatgaggatc
     4261 tgcaggcgtc gtccggtatc ggcgtaggga tgcccgccat tcttttccat gcaggcttcc
     4321 agcggccgga tatagtattc ctccagcgta cggcccgctg agacctcctg gcggtcgtgt
     4381 ccgacgataa cccggccgtc caccagccat atatcggctt cgatggaacc gaagcgcgcc
     4441 tgccaggcgg accaaaaggg gataggctgc tcataatcgt tatgagaatg cgtattggct
     4501 acagtgtagc ggacaggttg cgcggatgcg cgtagggcga cgaagagaag gaagaggaaa
     4561 atgtttcgca ttcagcaaat ataagggaga gaggggtgac tgctgcatcg ttgcgccttg
     4621 ccgttaggga cagttacgat gcggtttgga tgggtcgggt gcggaaaata tgggcgcaaa
     4681 ggtatcgtat accccgttag taatattggg ttcatacaaa tcttacaatt tcttaacata
     4741 tgcttaaaat accaataata tcttatttgg aactttgaaa aaccacaata aatggaaaag
     4801 cgacccgtag atgtagttgt catttcggac gttcacctgg gaacatacgg ctgccgggcc
     4861 aaggagctgg tcaactacct caaaagtatc accccgaaca tacttatcct caacggagac
     4921 attatcgacg gctggcaatt caccaaacgc tatttcccgg tagcccatat gatggtcatc
     4981 aaggagatca tccagttcat cacgagcggg acaagggtct tctatatcac cggcaatcac
     5041 gacgagatga tgcgcaggta tacggatatc cagatgggca atttcacgtt gaccgacaaa
     5101 ctggtcctgg agatcgacaa caagatgacg tggatattcc atggagacgt tttcgacaat
     5161 acgaccaagg gcggtgcaag gatactggcc aagctgggca gtagcggata taatctcctg
     5221 atccggttca accgcttcat caatttcatc ctgaaagcgc tgggtcgcga gcgggtgtcc
     5281 atttcgaaaa aggtgatggc gggagtcaat aaggcagtag ccaagatcaa cgatttcgaa
     5341 gctatcgctg ccgagctggc catcgggaag aagtacgatt atgtcatttg cgggcatatc
     5401 catcagccac agaagaagat agtagagacg aaagacggga aggtgaccta tctcaattca
     5461 ggggattggg tggaacacct gacggcgttg gaatatcagc agaatcagtg gtcgatcttc
     5521 gaatacgatg agaagaactt ccctgtgacg cagaccgtcg agatgaagcc ttcggtgagc
     5581 gtattgacgg acgagatcag tttttatatc aattcactgg cagcaattta aggtttatga
     5641 agatttttta cgcgatacaa gcaacgggca acggccatat cagcagggcg atggaaatga
     5701 tcccctacct ggatcgttat ggcaaggtgg atatttttct gagcggtgcg aacaacacgc
     5761 tggagctgga tgcacccgta aaataccgga gtaagggttt gagccttttc tacacccata
     5821 gcggcggtct caattactgt gagatcgtcc ggaaggtgtc accattgcgg ttgtggcggg
     5881 aagtccgcga tctgccggtg gagaaatacg atctggtcat caacgatttt gagtgtatca
     5941 cggcgctggc ctgcgcacat aagcgggtgc cctcggtaca attcgggcat caggcaagtt
     6001 ttgtgtctcc gaaggtgccc cggccggagc atcgggaacg aatgggggaa tggatccttc
     6061 gcaactatgc gcgtgcgacc caatatatcg gtctccactt caggcaatat gacgacttca
     6121 ttttgccgcc cgtgataaaa aaagagatat tgcaggcgga tccggaggac aacggtcata
     6181 taaccgttta tttgtcgagc tatagtgacg ctacggtgaa acgctacctc cagccgctga
     6241 aggagttttc tttccaggtg ttttcgaaag aggtgcatac acccgcgcgg gacgggaata
     6301 tcctgttcat cccggttagc aaaggagcct ttaacagaag cctgatcacc tgtaaggcca
     6361 tcctcacggg ggccggtttc gaaacgcccg ccgaggccct gtatctcggt aagaagctga
     6421 tggtgatacc cattcggggg caatatgagc aattctgcaa tgcggccgca ttatccaaaa
     6481 tgggggttcc tgttctacat gcgctggacg cgggtttcga cggggcattc cgcggatgga
     6541 tggcgcagaa aaggcggcct catctcaatc tcgattattc caccgagtcg atcgtaagct
     6601 atctgatgca tcattgcgga cgggggaagg cggatgcgtt ggatatgttg tatccggatt
     6661 gtgtgttcaa ctagcaggcc gctaccgatg tcacccaaaa aagccccttc atggaaatga
     6721 aggggcttct gcatgagaaa attcaactct acatatataa accatcgatt aacaatgctg
     6781 tcgatgcgat tgctggaaat ttatcgcatc tccagcattt tcgcttatgt ctgtccctcc
     6841 gggacaagcc gcctccggcg gcttcggaag gatatacaaa aataaactcc ccgtattacg
     6901 aggagtttac tgaatagtta cgtctatatt atcaaacgaa tttattgcgg tgctttaata
     6961 ccttggcttc gagatagaag ataatctctt cggcaatgtt cttggtctgg tcgccgaccc
     7021 gctccaggcg gcggatgatc gaaagcagcc agagggcctg tgaggcgtct tcgggatgtt
     7081 ccatgatatg gacctcgagg atatggtggg actttttgtt cacatcatcg agcagatcgt
     7141 cctttttgaa gacgcggcgg gccaggatcg tatcttcttt ttcgaacgcg acctgtgtgt
     7201 cttccagcat attgacggct tcctcgtaca tcggcaggat attcgtgatc ttgagcatct
     7261 ggtccgaaaa tggggcctcc gcgtcgatga catatttggc gatgccttcg gcatagtcgc
     7321 cgatgcgctc gagattattg ttgatcttca ggatggccag caggaagcga agatcgacgg
     7381 ctacggggca gaacaaagcg aagatattct cgcaatcgcg gtcgatcttc agctcgttgc
     7441 cgttgaccct tttctccttc agcactactt cacgcgccag gtccttatcg aagttcagga
     7501 aggcctgctg cgccttgctc aattgggaat tgaccagggt ccacatattg atagcttcgg
     7561 tcttcaaatt ctgtaattcg gtttctattt gtgccatggg atgggtaaaa gttatgtaaa
     7621 tataattaat ctattgacgg gtgccagtag ggcggacggg tgtccgcgac taactgaaac
     7681 gtccggtaat atagttctgg gtgcgattgt ctttagggtt ggtgaacatg gtcttggtct
     7741 catcgtattc caccagctcg cccaggaaga aaaaggccgt cttgtcgctg accctcgcgg
     7801 cctgctgcat gttgtgtgtc actacgatga gggtataatt ggccttgagc tcatggaaca
     7861 gctcttccac ggtggacgtg gagatagggt cgagcgcgga agtaggctca tcgaagagga
     7921 tgacttcggg ttccattgcc acggcgcgcg cgatacaaag ccgttgttgc tgtccgccgg
     7981 aaaggaagga gcctttcttg gtgagggagt ccttgacctc gttccatagt gcgacgcgtg
     8041 tgagggaatg ttcgacgatc cgatcctttt ccgccttggg gagcttgacg ccattgagct
     8101 tatagcctgc aatgacgttg tcgtagatgc tcagattggg gaaagggttg ggccgctgga
     8161 acaccatgcc gatcttcaga cggacgaaga tggggtgcat tttataaacg tcctcttcgt
     8221 tgagcagcat ttcgccgctg gagttagcgc cggggatcag ctcatgcatc cggttgatac
     8281 acctcaggta cgttgtcttc ccacagccgg aagggcccat gacggcgatg atgtgattgc
     8341 ggggaatatc gatgttgaca tttttgacga catgattggc gccgaatgaa gcattgaaat
     8401 tcctgctttt tataactgaa ctttccattt tcgtgttaag agcttagtaa gaatgtttag
     8461 aaataggacc cataccagga ggataaggga ggctccccag gccaggttga gccaatcggt
     8521 atagggactg gtggcatagt tgaagatcag caacggcagg ctttgcatag gtttggcgat
     8581 attgaccgaa aggtacggat tcccgaaggc ggtgaacagc aacggggccg tctcgccggt
     8641 gatccgggcg atagccaaca tgaccccgga gagaatgccg ctgataccca cggggacgat
     8701 gaccttgaac atgacccggt ggaagggcac gcccagcgcc agcgccgctt ccttgagcga
     8761 gtcgggcaac agcttgaggg tctcttcggt gcttcggacg atgatcggaa gcatcatgat
     8821 ggccagcgca acgctcccgg agagtgcgga gaaaccacgg aggggcttga cgagccagaa
     8881 ataggcgatg atgccgatga cgatcgaagg tacaccttgt aagacgtcgg ttgagagtcg
     8941 cacccagtag gccagccggc ttttcctgtt ctcactgaga tatacgccgg ccatgatgcc
     9001 gatgggcact gcgattacgg cggctatcag gatgatgatg atgctaccga cgatggcatt
     9061 ggcgatgccg ccgccggttt cgccgacggg cttgggaata ttgaccagga aatgccagtt
     9121 gatcctggta ataccggcgc ggaagatata gaaaaggatc gccagcagcg gaacgacgat
     9181 cgcgaatgcc agaatatatg ttaggatctg gaagccgtag ctcttaccga gcctgtattt
     9241 gacgcttttc gcttgcatag tttttaatgg agggtgatgg gatggaggtt agcatagttt
     9301 ttactggcgg gtgatgggat ggagggtgat gggatggagg ttagcatagt tttaatcgtt
     9361 ggtgaagcga tcgatgaccc gtttgccgat caggttgatc acgacggtga ccaggaagag
     9421 caccaggccg agctcgatga gggccgaaag ataaaccggt ttgtcggctt cggtgaattc
     9481 attggcaatg acgctggcca tggtatttcc gggtgcgaag atgcttttgg gaatggcgct
     9541 ggaatttccg ataaccatgg tgacggccat cgtttcgccc agggcccggc ccagcgagag
     9601 gaggataccc gcgaacagac ccgacttggt atagggcagt atcacgctgc ggatcacttc
     9661 ccagcgggta gcaccgagac cgtatgccgc ctctttcagg tgagaaggga cgagggtgat
     9721 catggagcgc ccgagtgatg cggcgtaggg gatgatcatg atggccagga tcaggctgct
     9781 gctaaagata ccgacgccta agggatcggc tccaacatga ccctcgaagc tccggaccat
     9841 cggcaccagc actgccagcc cccaaaagcc atagatgacg ctgggcacgg ctgcgataag
     9901 ctcgacggca ttcttgagga agttgggtac ccaccctttc gtatgatatt ccccgagaaa
     9961 gacggcgacc gcgatggaga aagggatcga gatgatgagc gccaggaagg acgtcagcaa
    10021 ggtgcccagc agaaaaggcc aggctccata aatatcctcg acagggttcc acgtcttgcc
    10081 ccagagatat ttgagaccga gggcctttat agaaggcagg gactggacga tcagggtaaa
    10141 gaggatgccg agcaccagga tgatcatgat cagtcccatg atcatcaggg tcttgttaaa
    10201 aatattttcc cagcggatct gcttgaaaga gcgggcaatc ctttttttcg ccttggcaat
    10261 ggcctccggc gaagaattaa ttacccgcag cggaaggtcg ctgcgggtaa tcatatcatt
    10321 ttctagagat gatgaatcca taagtgtcgt tcaattactg taagatcggc ttgccatcat
    10381 aagtgacggt tttcaactgt gcttctccgg cgtcgactgc ggctttggac agtggagcat
    10441 aggtcagggc ggagctgtat tgttgtccgt ccctgatcat ccaatcgata agcttcacca
    10501 gccgcgcagc gcggtcggcg ctgcggtcgc catatttctg ctctttgtaa agaagtaccc
    10561 aggagaaccc ggagatcgga tagccattgg cagcatccgt gttggtgagc gaaaccttgg
    10621 tgtcggccgg gatctgaata tcagctgcgg cgcttacggc cgggatgctc ggggaaatgt
    10681 agtttccgga tttattctgt atccgggcat acggcatatt gttctgaacg gcatatgcca
    10741 attctatata accaacggca ccgggagttt gtttaaccag accgccaacg ccctcggagc
    10801 ctttcccgcc gagacccgtg ggccagttga cggaagaacc tttaccaacc gtcttgttcc
    10861 agtcttcgct gatcttggac aggtaggtgg tgaagatgtt ggtggtgccg ctaccgtcgg
    10921 aacggtgtgc aacaactatg cccgtggaag gcagcttgac acccttgttg atggcggcga
    10981 tcttcgggtc gttccatttg gtgatcttcc ccaggaagat gtttgcgagt acgtcggcgg
    11041 tgaattggag cgtatccttt acatcgggca ggttgtaggc gatggctacg gcgcctgcgg
    11101 tgatcgggat gtgaacgacc gggccgctga cggcggaatc ctgtttgcca ttcatcgggg
    11161 cgtcggaagc gccgaagtca accgttttgg cagtcagctg ggcgatacca gcacccgaac
    11221 cgaccgactg ataattgatc ttcaggccgt ttgtcttatt gtattcggaa aattgtttcg
    11281 agaacagggg attgtcgaat gagctgccgg cgccgattag cgtattgtca tcgccggatg
    11341 cggaagccgt gctgtcactg ccgccggagg taccgccgcc gcaggagatg agactaacgg
    11401 tcaggatggc gaaggaggcg ccggccagaa gggcgttctt aatgctgaaa atgttttttt
    11461 tcatatatta ttactgttaa ggagttctta gatcaattat ccgattagtg caaatacggg
    11521 taatatgaat accctgggtt tgcaaacagc ttaccgaacg taaagtagaa ggtcacacgg
    11581 tatacaaggt catagtccct ggccgctctt ccggtgagat tggcctgctg tccgatatat
    11641 ctgtcatacc agatattcgg catgatatga atatttgcca ccggagaata atcgataccg
    11701 gtgctgagga acctttcctt attgttcggt tcgtatgacg tacttacacc atacagggaa
    11761 gcatacctgg tataagtggt attgtcgtat ttggtattcg gattgtaaaa atcataccgt
    11821 gcccaaaagc gcagcctggt cccgacgaga ttgacatgac cgtagaggct aagaccggtg
    11881 gcgtttccgc tcaatgtgtc ttttccgccc gccctggtac ctacctcgga attgcccagc
    11941 gtgttgatat atccttccgc gccgatggta aatttcttat cgacccacgc gatgatccct
    12001 tttgtcatgg cacgataggc gtggccataa gtggtgggaa gccagttctg acgctggtaa
    12061 tcctggtaaa tatcgaagaa cagcctgcgg ttggcaacag caacgaaggc gtccccgtac
    12121 caccacttgt aaacctccgt attcagggcc tggggcttgg cgctggagcc gttggctaca
    12181 aggaagtcgt atccataagt tgtcgattta tcatccgact gatagcggcc ttgcaaaccc
    12241 acgccgaagt cgaaggacgg tgtccggcga acatcggtga tggtcctttc gacaggtcgg
    12301 gaatagctga aaatattttc cgaagtgtaa gggaaagagg gagttggttg aagaccgaat
    12361 acgagatcgt tacctttcca caggaaatcg tgccagcgga tattggcaaa cttgacataa
    12421 aaagtgaatt tattgtctgc cgccaggtcg ccggtacctt gtgtaggggc gatgaaatcg
    12481 tcttccgccg ccaacagcag ttccgcgctg aattttttgg atatattgta atcgtatccg
    12541 aggtagatac ggcggaattg gaattggtca atgtcctgtt ttattccgct gtattgatta
    12601 tttccgcccc ggttgaggga atcgctgtga gctttatgga aataatcccc gaaggcatag
    12661 ccccagatac ggcccgagtt gggaacaccc gccttaaatg ccgagtcgga attccagagg
    12721 ctggtgcctt gaccggaatt gaaaaggtat tgggcctgcc cggtaatggc cagacagaac
    12781 agcagtgcga tcaaaaggaa ttgctgatag aagttgcgtt tcataaaagt cctggttctt
    12841 taagattaga attttttcag tagtcgttga atccgctgca aagcaatgcc tatgcaatga
    12901 cccgaatgtt accggaatat taccttaatg ttaagccctg tgacaattta gtcagcgggg
    12961 atccataatc atttgataat tagctattgg ccagatactc caattatttc ccaaattgag
    13021 gagtagattt gggatcataa gaaacttcct ggtatatgcg cttgtcaaga cccttcttcc
    13081 ttattatagt ttgcaccgtg gcggccctac tgatccggcc gcgaagatca ctcgcccaaa
    13141 aacggacgct ttccatcggg gatgtcctga ctcttgtaga atcggggcaa ccgcaattgc
    13201 aggcatacaa agaacaagcc gcggccgccg gttataacgt caaccttgcc agaaacacgc
    13261 ttgtccccaa cctcacagga ggataccagg ccggttatgc caccgacaac aatatcgccg
    13321 gtctcagcta tccgggattg t
//
LOCUS       DASYSV010000269         4840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1072061, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000269 DASYSV010000000
VERSION     DASYSV010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072061"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..513)
                     /locus_tag="VGQ51_08250"
     CDS             complement(94..513)
                     /locus_tag="VGQ51_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEV9036596.1"
                     /translation="MQKKIDELLKKNHLSITGGRRKILELFLQQDGALSHSDIEKRAG
                     EKFDRVTVYRTLQTFLEKGLIHGIPSPDNSLRYALCKEDCSGGHHHDDHVHFICTSCG
                     ITVCLEEVMIPPIKLPGGYVSEQVEMLISGVCKTCNR"
     gene            complement(521..1180)
                     /locus_tag="VGQ51_08255"
     CDS             complement(521..1180)
                     /locus_tag="VGQ51_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEV9036597.1"
                     /translation="MKKKLLYMAGFFILLFLGFYFALARWIPGFGKPVLPVLSEVPTF
                     SFIDQEGKEITGKDLAGKVYVAEYFFTTCRGICPKLNNNLKKLAGDFVGEKDFRLLSF
                     TVDPETDSVGRMKHYADSLGADPARWFFLTGRKDSLYHLARNGYLLDDPKNNLTNINE
                     QFLHTQFIALVDRGGRVRKIYDGLKKDEMDELEKDIPTLLKEKASDARFVPSLYNNNP
                     G"
     gene            1286..1627
                     /locus_tag="VGQ51_08260"
     CDS             1286..1627
                     /locus_tag="VGQ51_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HEV9036598.1"
                     /translation="MDALGATVSLVCAIHCIVLPLIAAALPLVSVNIFFEYAMIGLAF
                     LIGLGALLHGYRKHHRRPAPGLLFTIGMILLIAKQIWQGQEFILLLMALPLILCAHGL
                     NYRLCRRAQIR"
     gene            1655..2248
                     /locus_tag="VGQ51_08265"
     CDS             1655..2248
                     /locus_tag="VGQ51_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEV9036599.1"
                     /translation="MIKTGNPVISIYTEMTPNPETMKFVANKLLYPGKIIDFPDVEAA
                     KPSPLAIELFGFPFIKSVFIASNFVTLTKTPDTDWDDVIPAIRQFLKEYLEEGKPVIN
                     EEEIVTRKAEGNAIAADDDDVVKRIKELLENYVKPAVEMDGGAIQFKSYHEGVVNLML
                     QGSCSGCPSSMITLKAGIEGMMKRMIPEVREVVAESE"
     gene            2248..2784
                     /locus_tag="VGQ51_08270"
     CDS             2248..2784
                     /locus_tag="VGQ51_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9036600.1"
                     /translation="MSLKKIVVTGPESTGKSSLCDALAAHFHTSWVPEYAREYLETHG
                     VAYTPEDLSHIARGQVALEDRRAAIAEAAGHRFLFIDTDLYVIRVWSEFVFDRCDPWI
                     LNQIAIRRYDAYLLCRTDLPWEKDDLREYPDPAIRERLFRIYREILIRQSTPWMEIGG
                     HGDARIAAGIEAVHLLLK"
     gene            complement(2773..3474)
                     /gene="mtgA"
                     /locus_tag="VGQ51_08275"
     CDS             complement(2773..3474)
                     /gene="mtgA"
                     /locus_tag="VGQ51_08275"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEV9036601.1"
                     /translation="MPSKSQGTMARVWRIFKRVVIFLFFFQFFYILLLKWVNPPITLT
                     QLGSWVTGNGLRRTYVPMKDISPYAKLAVISSEDQLFPDHDGFDFKSIEKAMKHNQKS
                     KSLHGASTISQQVAKNVFLWQGRSWMRKALEVYFTFMIEWIWGKKRILEMYLNVSEMG
                     KGIFGIGAASHYYYNIPAKSLTRIQAARIAACLPNPKVFTIRPLSQHVATRSPWIIGQ
                     MGILQNDTDIQKLLQ"
     gene            complement(3500..3994)
                     /locus_tag="VGQ51_08280"
     CDS             complement(3500..3994)
                     /locus_tag="VGQ51_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036602.1"
                     /translation="MRKLSALILLFYIIPSFCKAQHDILVLEKRGMHVRTYTIGDPMT
                     FRTVYDQWFTGTIDDLHRDTVFIAGQSFSYKEIAAISRIKTKWDNRTTGAILTMAGIG
                     FFAIGAINGALRGDEAKEWYTTSGVIIGGALITAGVVFLATSKKYYRLGGKYKLQYLQ
                     IGRR"
     gene            complement(4007..4723)
                     /locus_tag="VGQ51_08285"
     CDS             complement(4007..4723)
                     /locus_tag="VGQ51_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEV9036603.1"
                     /translation="MESVLKGLALGFVLALSVGPIIFTIIKQSLNSGHKGGFSFVAGV
                     WVSDILLVVMSNTVTALVSQALEHTRVIAFGGSVFLIALGVYFVFFKKVTLGKDPSGD
                     DTRFRKRDMARVFASGFLINTLNPGVIFFWLGNATVLSLNHTLQERIIIFSVCLLVNM
                     SADVGKVMMAGKLRSKLTLKTLKLINRISGLILIGFGIALLWGVIFYHPPKKNEMHLS
                     GFVRPRSVGSALQVINNRLS"
BASE COUNT         1183 a         1309 c         1160 g         1188 t
ORIGIN      
        1 ttcaaaatag ctactaatca gagccttaac caaaagtccc ggtgtttcta catcggggct
       61 ttctttttta tgcaggtgaa gcgatcgttt acctcaacga ttacaagtct tacacactcc
      121 gctgatcagc atctccacct gttcgctgac atatcctccg ggaagtttga tgggcggtat
      181 catgacttct tccaggcaga cggtaatacc gcagctggta caaatgaagt gcacatgatc
      241 gtcatggtga tgaccacctg aacaatcttc tttacataac gcgtagcgaa gcgaattgtc
      301 cggtgatggt atgccatgga tcaacccttt ctcgagaaat gtctgcaaag tcctgtatac
      361 ggtgacgcga tcgaattttt caccggcgcg cttttcgata tcgctatgcg acaacgctcc
      421 atcctgttgc aaaaaaagct ccagtatctt ccggcgtcca ccggtgatgc ttaaatgatt
      481 tttcttcagg agttcgtcta ttttcttttg catagctgga tcaacccggg ttattgttat
      541 agagactggg gacgaaacgg gcgtcggaag ccttttcttt cagcagggtc gggatatcct
      601 tttccaattc gtccatctca tcttttttca acccgtcata gatcttccgc acccgcccgc
      661 cgcgatctac caatgcgatg aattgggtgt gaaggaactg ctcgtttata ttggtcaggt
      721 tatttttcgg atcatccagc aggtagccgt tgcgcgccag atgatagagg ctgtccttcc
      781 tgccggtgag gaaaaaccaa cgcgcgggat cggcgccgag cgaatccgca taatgcttca
      841 tccttcccac gctgtccgtt tccggatcca ccgtaaaaga aagcaggcgg aaatccttct
      901 cgcccacaaa atcccctgcc agctttttta aattgttgtt caacttgggg caaatgcccc
      961 tgcaggtggt gaaaaaatac tcggccacat agaccttgcc cgccagatcc tttccggtga
     1021 tctccttccc ttcctgatca atgaaggaga aagtcggcac ctcgctaagg accggcagga
     1081 ccggttttcc aaaaccgggt atccatcggg ccagggcaaa ataaaatccc agaaacaaca
     1141 ggataaagaa cccggccatg tagagcagct tcttcttcat ttcacaaagg taactgctta
     1201 tggccataat taatttgcaa caacgttgca tttgccttaa ttttgccgga tgttcgcccg
     1261 taagatcaat cccaaaaata tcaatatgga cgcgctcggc gccacggttt cactcgtctg
     1321 cgctatccat tgcatcgtcc tgcccctgat cgccgcagcc ttaccattag tgagcgttaa
     1381 tattttcttt gaatatgcca tgatcggcct ggctttcctc atcggcctcg gggctttgct
     1441 tcacggttac cggaaacatc atcgccggcc cgcgcccggc ctgcttttca caataggaat
     1501 gatactgttg attgccaagc agatatggca agggcaggag ttcatcctcc tgctgatggc
     1561 cctccctctt atcctatgcg cccatggctt gaattatcgg ctttgccgga gggcgcagat
     1621 tcgttaattt tgcagtatta aactcggcga aatcatgatc aaaacaggca atccggtcat
     1681 cagcatctat accgaaatga cccccaatcc ggaaacgatg aaattcgtgg ccaacaagct
     1741 attgtatccc ggtaagatca tcgatttccc ggatgtcgaa gccgccaaac cttctccgct
     1801 cgccatcgag ctcttcggct ttccctttat caaaagcgtc ttcatcgcca gtaatttcgt
     1861 gacactgacc aagactcccg ataccgactg ggacgatgtc attcccgcca ttcgccaatt
     1921 cctcaaggaa tacctcgagg aaggtaaacc ggtgatcaat gaagaggaga tcgtcacccg
     1981 gaaggcagag ggcaacgcca tcgccgcaga tgacgacgac gtagtcaaac gcatcaagga
     2041 attgttggaa aattatgtga aacccgccgt cgaaatggac ggaggcgcca ttcagttcaa
     2101 aagttatcac gaaggcgtcg tcaacctcat gctgcagggc tcctgctcgg gctgtccctc
     2161 ttccatgatc acgttgaagg cgggtatcga aggcatgatg aaacgcatga tccccgaagt
     2221 cagggaagtc gttgccgaat ccgagtaatg tctctaaaaa agatcgtcgt caccggaccg
     2281 gaatcgaccg gcaagtcttc gctatgcgat gcattggcag cgcattttca taccagctgg
     2341 gttcccgaat atgcccgcga atacctcgaa acacatgggg tggcgtatac gccggaagac
     2401 ctctctcaca ttgcccgtgg acaggtggcc ctggaagacc ggcgcgcagc tatagccgaa
     2461 gcggcgggtc atcgcttcct gttcatagac accgatcttt atgtgatacg cgtatggagc
     2521 gagtttgtct tcgaccgctg tgatccctgg atcctcaacc agatcgccat ccgccgttac
     2581 gacgcctatc tgctttgccg gaccgatctc ccctgggaaa aagacgatct gcgggaatat
     2641 cccgatcctg ctatacgcga aagactattc cgcatctacc gcgagatcct catccggcaa
     2701 tcgactccct ggatggagat cggcggacat ggcgacgcac gtatcgctgc gggaatagag
     2761 gcggtgcacc tgctattgaa gtagtttttg aatatcggta tcgttctgca ggatacccat
     2821 ctgtcctatg atccatggac tgcgggtcgc gacatgttgc gacagcggcc ggatcgtaaa
     2881 gaccttcgga ttgggaagac aggccgcgat cctcgccgcc tgaatgcgtg tcagtgattt
     2941 ggcgggtatg ttataataat aatgggaagc ggccccgatg ccgaaaatgc cctttcccat
     3001 ttcgctcaca ttgagataca tttccaggat ccgttttttt ccccatatcc attcgatcat
     3061 gaaggtgaaa tatacttcga gcgccttgcg catccagctt cggccctgcc agagaaaaac
     3121 gttcttggct acctgctggc tgatcgtact ggcgccatga agcgatttgc ttttctggtt
     3181 gtgcttcatg gctttttcga tggacttgaa atcgaaaccg tcatgatcgg ggaacagctg
     3241 gtcctcgctg ctgatcacgg ccagcttggc ataaggagaa atatccttca tcggcacata
     3301 agtacgccgc agaccgttgc cggtgaccca gcttcccagc tgggtaagcg taataggcgg
     3361 attgacccat ttcagcagga gtatgtagaa aaattgaaaa aagaacagga agatgacgac
     3421 gcgcttgaaa attcgccaga cgcgggccat cgttccctgg gatttagaag gcatccgata
     3481 gatttaccac cacaatatac tatctccgcc cgatctgcaa atattgtaat ttgtatttcc
     3541 cgcccagcct ataatatttc ttcgaggtcg ccagaaagac aactcccgcc gttatcaatg
     3601 cgccgcctat aatgaccccc gaggtcgtat accactcttt tgcttcatca ccccgtaatg
     3661 cgccattgat agcgccgatg gcaaaaaaac caatccctgc catcgtgaga atggcccctg
     3721 tggtccggtt gtcccatttg gttttgatcc gcgaaatggc cgcgatctcc ttatagctga
     3781 atgactgtcc cgctataaag acggtgtccc tgtgcagatc atcgatcgtc ccggtgaacc
     3841 attgatcata cactgtccgg aaggtcatcg gatcgccgat ggtatacgtg cggacgtgca
     3901 ttcctctttt ttccaacacg agaatatcgt gttgcgcctt gcaaaaagag ggaatgatat
     3961 aaaatagtaa tatgagcgcg gatagttttc gcataccgat ccaaacttac gatagcctat
     4021 tgttaatgac ttgtaaagcg gagccaacgc tgcgtggcct gacaaagcct gataaatgca
     4081 tctcgttctt tttcggcggg tgataaaaga tgacgcccca gagtaacgcg atcccaaaac
     4141 cgataaggat cagtcccgag atacggttga tcagcttaag tgtttttaag gtcagcttgc
     4201 tgcgcagctt gccggccatc attaccttgc ccacatcggc gctcatattc acgagcagac
     4261 aaacggaaaa gatgatgatc ctctcctgaa gcgtatggtt gagggatagt accgtcgcgt
     4321 tccccagcca aaaaaaaatg acgccggggt tgagcgtgtt gatgagaaaa ccggaagcga
     4381 acacgcgcgc catatcgcgt ttccggaaac gcgtatcgtc cccggacgga tccttgccaa
     4441 gcgtcacctt cttgaagaat acaaaatata cgcccagcgc gatcaggaag acgctaccgc
     4501 cgaaggcaat gacgcgcgta tgctccagcg cctgcgagac caatgcggtg acggtattgc
     4561 tcatgacgac cagcaggata tcgcttaccc acactcccgc tacaaacgag aaacctccct
     4621 tatgaccact gttgaggctt tgcttgatga tggtaaatat gatgggacct accgataagg
     4681 caagaacgaa ccccagcgcc aatcctttca ataccgactc catatgatgt gtcctattac
     4741 gagttagagg cttccggtct ccagaaattt ttgccagtcc agtaacatct tgcccttcag
     4801 cacgcggggg aacttatgtt gcccgccgat cttccctttg
//
LOCUS       DASYSV010000270         8716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_50777, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000270 DASYSV010000000
VERSION     DASYSV010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50777"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            968..1168
                     /locus_tag="VGQ51_08290"
     CDS             968..1168
                     /locus_tag="VGQ51_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036604.1"
                     /translation="MRELHCLLVGNLLLIGEGQSTQNPTPFIKNSKGVTGSNDEFRSA
                     FIHYTIRTSYQVTLFKECVTQC"
     gene            1381..4572
                     /locus_tag="VGQ51_08295"
     CDS             1381..4572
                     /locus_tag="VGQ51_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9036605.1"
                     /translation="MRRLHCWLMGICLLMGLGLFGQTSPVTGRVTDPSGTPIPNATIR
                     IKGVKGGTSADANGSFKIKAAGNATLVVSAVGYEQAEIPIDHRTAVTLMLRPSNLALS
                     EVVVTALGVRRDKKILGYSVTTVGKQDVELRPEEDVTRILQGKTPGVDILATSGISGS
                     GTNISIRGISTISGGPSTPLFIVDGVPFDASSNVNNNFQYGGGISASSRFLDLDPNDI
                     ESVSVLKGLAAATIYGEAGRNGVILVTTKGGSGRKTNKKMEITGSQSIFTNKVANLPD
                     YTSKYGGGFDLAPSAAFSNWGPLFTNPPLQVAYSPSLVAAFPQLYSGKTQDFKNYANN
                     VSGFFRTGLLSTTSVNVATSGQNSTFNANYTYLSDDGFTPNNRVYKNNFGLGGTAKLA
                     NNFSANAVLNFAITDMQTPTVGANGGGGSADVASVFGDLIFTPRSVNLIGLPFQNPVD
                     GSSVYYRNGNDIENPLWTAANDVSSDKTYRTFGTFGLKYDIMKGLSVNYRFGLDFYQE
                     YQTLDVNKGGKQGGAAYVGGLYRTTNIQNSIINNTAFVQYSTPFAKNFQLDAQAGFDF
                     RHDHYNQNGLKSTQQIVFGLFDHSNFISHDIYDEGGGDLDYRSEEIREGVFGQATIGY
                     KEWIYLTGTARNDWVSTLEPAHRQQLYPGISMSFIPTSAISALEGSKTVNYLKLRLSY
                     ATSAHFPTPYNTRPYINLSTNAFVDKGGNVINIASIPSQVPNPDLKPELITEEEAGIE
                     GRFFDSRVSIDLTGYFRTAKNQILNRQLDASTGYSYTTINAGAVDNKGIEASLTVTPI
                     RSKDWTWDLTGNFYLNRSKVHDLPVDIKQIELNGGGYSNRGGFAINGKPLGILEGSYT
                     LVDPKSGKKEVDNNGYYLGAPDIAVIGDPTPRYKLTGISTLTWKYISFRIQVDYTHGG
                     AMWSNTARTMLARGVTKDTDFDRTLPYTIPNTVKQDGTPNDIQQSVDNIYFNSYGFGP
                     DGESVWDATVIRLREASLSFSMPAKMLKNTPFGAASLSISGTNLWYLAPYFPKYTRFD
                     PEVNSLGITNAKGLDFFAGPSSRRLGASLRLSF"
     gene            4590..6281
                     /locus_tag="VGQ51_08300"
     CDS             4590..6281
                     /locus_tag="VGQ51_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEV9036606.1"
                     /translation="MRSKLNRLIITAFCFPVVFFLGSCNKQLSPLLNNPSVPSTSAAS
                     PDLYLNNLQLAFKNFFQNATDITDQMTRQEIMYGPTYYNAFAPTSFDGIWQTAYESVM
                     LTANTMIPISESKGETIHAGIAQVLKAYTMITLVDLFGDIPYSQALHGVAVPNPVVDK
                     GAAVYDSAEALLDSAIANFAIKASANPSNDLFYGGSAKNWTRLANSLLLKAYIQTRLV
                     DNSVTAKIAALVTANNLVTSASQDFVFGYSTHSQAPDARNSHYVGNYGTDNGAGDYIG
                     TWFMWELADAKGFQDPRTRYYLYRQCTDVYSDPRTASQTTRQFAIPCLYRTDPYAPGV
                     PYCLVDNGYWGRDHGNNEGIPPDNSLRTTWGLYPAGGAFDYDQNLAIGQSTGRESGAG
                     GGGINPIWLSSFTYFLEAEAALILGTPGDPKALTISGVDASINKVAGFPAAIGYKLPT
                     SDTTMLITATNTAKYENAVGTQYSAASTTAAKLNIIETEYYLAAWGNGLETYNNYRRT
                     GCPNNFQPTLDPNPGLFIRSFFYPSVYANFNKNAVQKTTTNVKVFWDNNPDNFVY"
     gene            6292..7173
                     /locus_tag="VGQ51_08305"
     CDS             6292..7173
                     /locus_tag="VGQ51_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036607.1"
                     /translation="MKRFIISVAGIGCLFVLIHGCAKKTISGDYKNLILGSYVTLDST
                     INNILDVGNSGSTVSIMVGKTVGSAVSSINIYVATGSNSEDTTGWKLIKSVTYSSGAV
                     LSVSTAELTKALGIAPAPGNKYTLQNQVVTKDGRRYATNNTPDTYNSFPAYNMALTWY
                     ATCVCAFTGNMAGDYYLTKDVWNDYGASGSNPVLIPGAVKDGPGANQISIYVYPGAPA
                     GGVETGPMIVNVNPVTGAATIVKSDIGNYGGPSSETTVTGKGFVFDCVGVIDLSITFN
                     YGGSPYANQEMILQKTP"
     gene            complement(7208..>8716)
                     /locus_tag="VGQ51_08310"
     CDS             complement(7208..>8716)
                     /locus_tag="VGQ51_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEV9036608.1"
                     /translation="LLEDKKLVELHHEKADASFAVGDLYLGKVKKLIPGLNAAFVDVG
                     FEKDAFLHYTDLSPYARSLLKFTGQAMNDSSTGGFDFGSFQVEPEIIKTGKINEVLNG
                     KPHILVQILKEPIAAKGPRLSCEISLPGRFVVITPFNDIVAVSRKIHSSDERKRLQKI
                     VEAIKPKNFGVIVRTAAEGKNTAELHEDLTDLVATWKMIQHNLKGAVAPAKILSEQTK
                     TTSMLRDLLNEDFNKIVINDRNIFNDTRSYIQRIAPDKIDIVSYYHNGSPIFDQFSVT
                     KQVKAAFGKTVNLPSGAYLIIEHTEALHVIDVNSGYKSVSNNQEQNALETNMEAAEEI
                     ARQLRLRDLGGIIVVDFIDMKLPDNKRKLIEAMENFMRTDRAKHAVLPISKFGLMQIT
                     RQRMKPEVSINTQEVCPTCNGTGKISSALILEDEIEKNLSYLINHQHKDLILAVHPIM
                     HAYLTKGRLFGIGSRQWNWHWKYKQKIRIRANTNYHLTEFHFFDKQEDEIKL"
BASE COUNT         2169 a         2343 c         2108 g         2096 t
ORIGIN      
        1 cctaaccatt ttgcgctctc atatccgcag ttgctgcaaa aaaatgccgt tcttatcttg
       61 ctcatgcggt aaagttagat gctaattcac gtgtttatga ttccctaatt tggaaagcgc
      121 gggataattg ataaaaaagg aaaagagatt gaaaaccggc tggcactttt tttaaaaaca
      181 aagggtaact ttttaaaagt aaaagggcca gctaaatcgc cagcccctta cttactaacc
      241 cattaaattc ttgcactaaa ttacaaatta gcgtcacatt gcaaaataaa tttttgatgg
      301 tgtgaataac tttgaagcct tttttagcgc gcccaaaatc cgaaacatca cctatataaa
      361 acactgaaga acaatgaatt atggggcttt gtaaaatgcc cctcgctcaa aggtctttct
      421 ttaaaggctt gaaaattaac ctgcctaaat tcacaaaaat tttttgaaaa aaatttgtcg
      481 gcggatcggg aacaaggaca tgaaaatttt catgcatacc ctgaagatgg tcaaaataat
      541 acctccggca tgcgattttt tgtgacccgg atatatttaa aaaaaccccc atttaaacct
      601 gccggccttc ggcctaaaca aatcattcct aattgcttgg cagcaatcat cccccaaaaa
      661 tcctaagcgg gcgcctcact gcatactatt ttccgtgaaa taaatttttc atgtaagagc
      721 agggacctaa tgccctgaca acaaagaaat tgcggattcg taaaaaacgc actacaacaa
      781 gttcataaaa aaatgttaag gttaaattaa tttctaatta aaaatgaact aatttaaatt
      841 gtcggagttg acatcggttt aaaaatgggg gatattgatt aataggctgt gcttcagtat
      901 tttacgttgt tgaccgcgag atgagatttc atagtgaagg ataattgttc actattaata
      961 actacacatg agagaacttc actgcttgct ggtgggcaat ctgctgctca ttggcgaagg
     1021 acaatctacg cagaatccca ccccattcat taagaacagt aaaggcgtta ccggctcgaa
     1081 tgacgagttc cgtagcgcct ttattcatta taccattaga acatcgtacc aggtaacatt
     1141 attcaaagaa tgtgtgactc aatgttgaca gtgaaagacg gccccggttt ctaccggggc
     1201 catatttttg cacttatgtt aattccttaa tctaattcaa aagatttggt atattcaagg
     1261 acttcactgg cacacggagt cttcttaatt cctgtctcgt agggttttca caaccactgt
     1321 cctacaaggg ttgcacaatg ggaacaaaat ccattggtat tacttcaacc ttaactacac
     1381 atgagaaggc ttcattgttg gctcatgggc atatgtctgc tcatgggtct tggcctattc
     1441 ggccagactt caccagtcac ggggcgagtc acggatccat ccggaacgcc tattccaaat
     1501 gcaacaatcc ggattaaagg tgtcaaagga ggcaccagcg ccgatgccaa cgggtctttc
     1561 aagatcaagg cagcggggaa cgcgacgctg gtcgtctcag ccgtcggtta cgagcaggcg
     1621 gagataccca tcgatcacag aaccgcggtc actttgatgc tgcggccatc gaatctcgca
     1681 cttagtgaag tggtcgttac ggcactgggt gtcagacggg acaaaaagat cctgggctat
     1741 tcggtcacca ccgtcggcaa acaggacgtc gagctccgcc ccgaagaaga cgtgacccgt
     1801 atcctgcaag gcaaaacgcc cggcgtagac atcctggcga ccagcggtat ctcgggttcc
     1861 ggcaccaaca ttagcattcg cggcatcagc accatctcgg gtggtccttc gactccactc
     1921 tttatcgtgg atggtgtacc attcgatgcc agtagcaatg tgaacaacaa cttccagtat
     1981 ggcggaggta tttcggcctc cagccggttt ctcgatctgg atcccaacga catcgaaagc
     2041 gtcagcgtac tcaagggtct ggctgcggcc acgatctacg gggaagcggg acgcaacggc
     2101 gtcatcctcg tcacgacgaa gggcggctcc ggacgaaaga ccaataagaa gatggagatc
     2161 accggctctc aatcgatatt caccaataag gtggccaatc tcccggacta taccagcaag
     2221 tacggcggcg gtttcgacct ggcgccttcc gcggctttca gcaactgggg tcccctgttc
     2281 accaatccgc ctttgcaggt agcttattca ccttcactgg tggcggcctt tccgcagctg
     2341 tattccggaa agacccagga ctttaagaac tatgccaata acgtaagcgg cttcttccgg
     2401 accgggcttc tcagtacgac ctcggtgaat gtagcgacct cgggacaaaa tagcaccttc
     2461 aacgccaact atacctatct gtcggatgat ggcttcacgc ccaacaacag agtctacaag
     2521 aacaatttcg gactcggtgg caccgccaaa ctggccaaca actttagtgc aaacgcggtc
     2581 ctcaactttg ccatcaccga tatgcaaacg ccgacggtag gcgccaatgg cggaggcggc
     2641 agcgcggatg ttgcttcagt cttcggcgac ctgatcttca cgccgagaag cgtcaatctg
     2701 ataggtcttc cctttcagaa tcccgtggat ggatcgagcg tctattaccg caatggcaac
     2761 gatatagaaa acccgctctg gacggccgcc aatgacgttt cctccgataa gacgtaccgg
     2821 accttcggta cgttcggtct caaatacgat ataatgaagg ggctttcggt taactatcgt
     2881 tttggcctcg acttttatca ggaataccag acactggatg tcaacaaagg cggtaaacag
     2941 ggcggcgccg cttatgtagg cggcttatac cggacgacca atatccaaaa ctccattatt
     3001 aacaatacgg cattcgtcca gtattccacc cctttcgcca agaactttca actggatgcc
     3061 caggcgggtt tcgacttccg tcatgaccac tacaaccaga acgggttgaa aagcacccag
     3121 cagatcgttt tcggtctttt cgaccatagc aacttcatca gccatgatat ctacgatgag
     3181 ggcggcggag acctggacta ccggtcggaa gagatcaggg aaggcgtttt cggccaggct
     3241 accatcgggt ataaagagtg gatctatctg accggcactg cacgcaatga ctgggtctct
     3301 accctcgaac cggcccatcg ccagcagctc tatcccggca tcagcatgtc cttcatccct
     3361 acctccgcaa tctccgcgct cgaaggcagc aagacggtca actatctgaa gctccgtctg
     3421 agctatgcta ccagcgccca cttcccgaca ccctacaata cgcgtcccta tatcaacctg
     3481 tcgacaaatg ccttcgtgga caagggcggg aacgtcatca atatcgcgtc gatacccagc
     3541 caggtgccca atcccgatct gaagcctgaa ttgatcacgg aagaggaagc cgggatcgaa
     3601 ggtagattct tcgacagccg ggtcagtatc gacctgacgg ggtatttccg gacggccaaa
     3661 aaccagatcc tcaaccggca gctggacgct tctaccggct atagctatac gactatcaac
     3721 gcgggtgcgg tggataataa aggcatagaa gcctcgttga ccgttacccc tatacgtagc
     3781 aaggactgga cctgggacct taccgggaat ttctacctca accgcagcaa ggtccatgac
     3841 ctccctgtcg atatcaaaca gatcgagctc aacggcggag gctattcgaa ccgcggcggc
     3901 ttcgccatca atggcaaacc gctcggcata ctcgaaggct cctataccct tgtggatccg
     3961 aagagcggca agaaggaagt cgataacaac gggtattacc tcggtgctcc cgacatcgcc
     4021 gttatcggcg acccgacacc caggtacaag ctgaccggca tttcgacact gacctggaaa
     4081 tatatttcct tccgaatcca ggtggactat acccatggcg gcgcgatgtg gtccaacacg
     4141 gcgcgcacga tgctggccag gggcgttacc aaagacacgg atttcgacag gacgctgccc
     4201 tataccatcc ccaatacggt caaacaggac ggtacgccca acgatatcca gcagagcgtg
     4261 gataatatct atttcaactc ctatggtttc ggtccggacg gcgaatcggt ttgggatgcg
     4321 acggtcattc gcttgcggga agcatcgctt tcgttctcca tgccggcgaa gatgttaaag
     4381 aatacacctt tcggtgctgc atcgctgagc atttcgggga caaatctttg gtacctggcg
     4441 ccatatttcc cgaaatacac gcgtttcgac cccgaagtca acagtctggg tattaccaat
     4501 gccaagggcc tggatttctt tgccggaccc agctcccgcc gtttgggggc cagcctgaga
     4561 ctatccttct aatctaaaaa tgaactacca tgcgatcgaa attaaatcga ctcatcataa
     4621 ccgctttctg cttcccggtg gttttctttc tgggaagctg taacaagcaa ttaagcccgt
     4681 tactgaacaa ccccagcgtg ccttcgacct cggcggcaag ccccgacctg tatctcaaca
     4741 atctccagct ggcgttcaag aattttttcc agaacgccac cgatattacc gatcagatga
     4801 cccggcagga gatcatgtat ggacctactt actacaacgc ttttgcaccg acgagcttcg
     4861 acggcatctg gcagacggcc tacgaaagcg tgatgctcac tgccaacacg atgatcccga
     4921 tctcggaatc aaagggtgag accatccatg ccggcattgc ccaggtatta aaggcatata
     4981 ccatgatcac gcttgtggat cttttcggcg atatacctta ctcacaggcg ctgcacggtg
     5041 tcgcagttcc gaaccctgtg gtagataaag gggcggcagt ttatgactct gcagaggcgc
     5101 tgcttgattc cgccatcgcc aacttcgcca taaaggcgtc ggccaacccg tccaacgatc
     5161 tattctacgg cggcagcgcc aagaactgga cgcgcctggc aaactccctg cttttaaagg
     5221 catatatcca gacccgtctt gtggacaata gcgtcacggc gaagatcgca gcattggtca
     5281 ccgcgaacaa cctcgtcact tcggcttcac aggatttcgt gttcggctat tccacacata
     5341 gccaggcccc tgacgcccgg aattcgcatt atgtcggcaa ctatggaacg gacaacggcg
     5401 ccggcgacta tatcggaacc tggttcatgt gggagctggc ggatgccaag ggcttccagg
     5461 acccccgcac acgttattat ctgtatcggc agtgtaccga tgtctattct gaccctcgta
     5521 cggcgagcca gacgacacgg caatttgcca ttccgtgtct ataccgcacc gatccctatg
     5581 cacccggcgt tccttactgt cttgtggata atgggtattg gggcagggac catggcaata
     5641 atgaaggcat tccgccggac aactcgcttc gcacgacctg ggggctatat ccggcaggcg
     5701 gggcattcga ttatgatcag aaccttgcta tcggtcaaag caccggccgg gaatccggcg
     5761 ctggcggagg agggatcaac ccgatctggt tgtcgtcctt tacttatttc ctcgaggccg
     5821 aagccgctct catcctgggc acaccgggcg acccgaaggc attgaccatt tcgggcgtcg
     5881 atgcctcgat caataaagtg gctggttttc cggcagccat cggctacaag ctccccactt
     5941 cggatacgac gatgctcata acggccacca acacggcaaa atatgagaat gccgttggca
     6001 cccagtactc ggccgcttct accacggccg caaaattgaa catcatcgag acggaatatt
     6061 atctggcagc ctggggcaat ggcctggaaa cctataacaa ttaccggagg acaggatgtc
     6121 ccaataattt ccagcccacg ctggatccga atccgggttt gttcatccgc agtttcttct
     6181 atccgtcggt atacgccaat ttcaataaga atgcggtcca aaagacgacc accaatgtga
     6241 aggtattctg ggataacaac ccggacaatt ttgtctatta aaaataaaca catgaaaaga
     6301 ttcatcatat ccgtcgccgg gatcggctgt ctgttcgtgc tgatccatgg ttgtgcgaaa
     6361 aagactatct ccggggatta caaaaatctt atcctggggt cgtatgtcac gctggattca
     6421 accatcaaca acatcctgga tgtaggcaat tcgggcagca cggtctccat catggtcggg
     6481 aaaaccgtag gatcggctgt atcctcgatc aatatctatg ttgctacggg ctccaattcg
     6541 gaagacacca cgggatggaa attgattaaa tccgtcactt attccagtgg cgccgtactg
     6601 agtgtttcca cggccgaact tacaaaggcc ctgggtatcg cgccggctcc tgggaacaag
     6661 tatacgctgc aaaaccaggt cgtcaccaaa gatggcaggc gatatgccac gaacaacacg
     6721 ccggacacct ataacagctt tcctgcctac aatatggcgc tcacctggta tgcgacctgt
     6781 gtctgtgcct ttacgggtaa tatggcaggt gactactatc tgacgaaaga cgtttggaat
     6841 gactatggag ccagcggctc caacccggtg ctgatccccg gcgcggtcaa ggacggaccc
     6901 ggcgccaacc agatatctat ttatgtctat cccggcgccc cggcaggcgg cgtggagacg
     6961 gggccgatga tcgtgaacgt taaccccgtc accggagcgg ctacgatcgt taaatccgat
     7021 atcggtaatt acggcggccc gtcgtccgag acgaccgtca ccggaaaagg ctttgtattc
     7081 gactgtgtgg gcgtgatcga tctctctata actttcaact atgggggatc gccttatgcc
     7141 aaccaggaaa tgatacttca gaagactcca taaaacaccg gggcgtccta aaaggacgcc
     7201 ccttttttta taattttatc tcgtcttcct gtttgtcgaa gaaatggaat tcggtgagat
     7261 ggtaattggt attggcgcgg atgcggatct tctgtttgta tttccaatgc cagttccatt
     7321 gccggctgcc gatgccgaaa agcctccctt tggtcagata ggcatgcatg atcgggtgca
     7381 cggcaagaat gaggtcctta tgctgatgat tgatcaggta acttaaattc ttttcgatct
     7441 cgtcctccag gatcagcgcg gaggagatct ttccggtgcc attgcaggta ggacaaactt
     7501 cctgcgtgtt gatgctcacc tccggcttca tccgctggcg cgttatctgc attaacccga
     7561 acttggaaat gggcagaaca gcgtgtttgg ccctgtccgt tcgcatgaaa ttctccatgg
     7621 cctctatcag cttccgtttg ttgtctggca gcttcatatc gatgaaatcc accacgatga
     7681 tcccacccag gtcacggagc cgcagctgtc tcgcgatctc ttcagccgcc tccatatttg
     7741 tttccagggc attctgctcc tggttgttgc tgacgctttt ataaccgctg ttcacatcga
     7801 tgacgtgcag cgcctcggta tgttcgatga tgagataggc gccactgggc aggttcaccg
     7861 ttttgccaaa ggcggccttt acctgcttgg tcacgctgaa ctggtcgaat atcggcgaac
     7921 cattatgata ataggacacg atatcgatct tgtccggggc tatccgctgg atataactcc
     7981 tggtatcgtt gaaaatattc cggtcgttga tgacgatctt gttgaaatcc tcattcagca
     8041 gatcgcgaag catgctggtg gtcttggtct gctcgctcag tatcttcgcc ggcgctaccg
     8101 ctcccttcag attgtgctgg atcattttcc aggtggctac gaggtcggta agatcctcat
     8161 gcagctcggc cgtattcttg ccctccgccg cggtacgtac gatcaccccg aaattcttcg
     8221 gtttgatggc ttccactatc ttttgaagac gtttgcgttc gtcggaggaa tggatctttc
     8281 tggagaccgc gacgatatca ttaaatggag tgatgacgac gaatctcccc ggcagggaga
     8341 tttcgcagct gagcctgggg cctttcgcgg caatcggctc cttcaatatc tgtacgagaa
     8401 tatgaggctt gccgttgagc acctcattga tcttccctgt cttgatgatc tcgggctcca
     8461 cctggaaact gccgaaatcg aacccgcccg tactcgaatc attcatcgcc tgacccgtga
     8521 atttcaacag gctgcgggca tatgggctaa gatcggtata atgcaaaaaa gcgtcctttt
     8581 cgaaacctac gtctacaaat gcggcattca gaccggggat cagctttttg acctttccca
     8641 ggtataaatc accgaccgca aagctggcat cagctttttc gtggtgtaac tctaccagtt
     8701 ttttatcttc cagtag
//
LOCUS       DASYSV010000271         3002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2226004, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000271 DASYSV010000000
VERSION     DASYSV010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2226004"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..2404)
                     /locus_tag="VGQ51_08315"
     CDS             complement(257..2404)
                     /locus_tag="VGQ51_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9036609.1"
                     /translation="MGLESELRKATRQATEKYLTLFNNMNQGFCIIEVIFDEKGKAYD
                     YRFLEVNPVFEKITGLNDAVGRTIKELQPAQEEHWFRIYGDVVKTGESIHFENAAAHL
                     AGGVWYEVFAIPFDRPETCQVAVFFNDITERKRRERQQEYLLKLTDALHLLSDPVEIR
                     RTAIRVVGEHLDASRAFYAEFEPDGSTYTIPDNYVSPGYSPRVGRFSGNAFPTVIRWF
                     LRGEMPVIDNVEDTPGLTDAEKEGSIAAGIHAFVGVPLIKGGRLVAQLGVQQDAPRKW
                     TDDELIVVQETADRTWAAVERAKAEEALRQSEELLNGQKEAFQAAMNGRPLAASLDVL
                     IRTAITQINNGARAAFFMIPPKKEGLHLVAGMSEEYARDVENFEVGPESMASGLAMHT
                     GRPVITPDVEKDASWVPWITLAHKHHYSAFWSFPVRAEGGPVVGTFALYFPGPRDPQP
                     KELEVAGVIAHAAAIIISRHMSEQNLHERELQLQLLVKQRDEFIGAASHELKTPVTSI
                     KAYAQIVQRELKQEEATENSDLMTRLNAQVNRLSVLINDMLDTTKISEGKLQLTVRPL
                     DVNELLKERVEEIQHTTAHRFDVRTSPLAPVMADRERIGQVITNLLSNAIKYSPDHTI
                     VTIVTCDTAGGIRVSIRDEGYGIPESDQPKIFDRFFRVTSNNMDRIPGMGLGLYISAQ
                     IIYRHGGTIEVQSRPGKGSVFSFTLPYQFSNKN"
     gene            2662..>3002
                     /locus_tag="VGQ51_08320"
     CDS             2662..>3002
                     /locus_tag="VGQ51_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036610.1"
                     /translation="MAVPKKDGKYPCLIFNRGGNRELGVLNDVQMVRLLGGPASWGYI
                     TIASQYRGNAGGEGQEEFGGKDVGDVLNLIPSFAEIAGADTTRIGGFGYGGCFYYHST
                     TASDGQCISTAG"
BASE COUNT          648 a          834 c          780 g          740 t
ORIGIN      
        1 gtggtcgatg ccggtccact ccagatctcg gccgacaaac aaaaaacggt caactggccg
       61 ccttatgccg gaggtttcct ggtcctcggc ggggtcatcc ttttggcgac cggtaataag
      121 aagatcggct aagcgccacc gcagaatggc cgccaccgaa agccgaatgg ctaagcgcca
      181 ccgcgcggca ggccgacccg gaaggagaat tggtaattgg ccaccccgcg gctcgccggc
      241 ccggaaggcg gaacggctaa tttttattgg aaaattgata gggtagggta aaggagaaga
      301 cagacccttt ccccggccgg ctttgtactt ctatcgtacc cccatgccgg taaatgatct
      361 gcgccgagat gtacagaccc agacccatac ccgggatccg gtccatatta ttcgaggtta
      421 cccggaagaa ccgatcgaat atcttcggct gatcggattc gggtatgcca tatccttcat
      481 ccctgatgct gaccctgatc ccgccggctg tgtcgcaggt gacgatggtc acgatagtgt
      541 ggtctggcga atacttgatg gcgttggata aaagattggt gatcacctgt ccgatccgct
      601 cgcggtctgc cataacagga gcaagcggcg atgttcgcac gtcaaaccgg tgtgccgtcg
      661 tgtgttgtat ttcttctact ctttccttta gcagctcgtt cacatccaat ggtctgaccg
      721 tcaattgcag cttgccctcc gatatcttcg tggtatcgag catatcattg atcaggacag
      781 aaagccggtt cacctgcgca ttaagccggg tcatcagatc gctattctcc gtagcctcct
      841 cttgcttcag ctctctctgg acgatctgcg cataagcctt gatgctggtg acgggcgtct
      901 tgagctcatg gctcgcggct cctatgaact cgtctcgttg tttgacgagc agctgtaatt
      961 gcagctcccg ttcgtgcaga ttttgttccg acatgtgccg ggagataatg atcgctgcgg
     1021 catgtgcgat cacacctgcc acttcgagct ctttgggctg ggggtcgcgt ggcccgggga
     1081 aataaagtgc gaaagtaccg actacgggtc cgccttcggc ccgcacgggg aaggaccaga
     1141 aggcactata gtggtgttta tgggcaagtg ttatccaggg tacccagctg gcgtcctttt
     1201 ccacatcggg agtgatcacc ggcctccctg tatgcatcgc cagaccgctt gccatagatt
     1261 ccgggcctac ttcaaaattc tcaacgtccc gggcgtattc ctccgacatg cccgccacca
     1321 ggtgaagccc ttcttttttg ggagggatca taaaaaatgc cgcccttgct ccattattga
     1381 tctgcgttat cgccgtccgg atcaaaacat cgagcgacgc ggccagcggc cttccattca
     1441 tggccgcttg aaaggcctcc ttttgcccgt tgagtaattc ctcgctttgg cgtagagcct
     1501 cttcggcctt tgcccgttct acggcagccc aggttctgtc agcagtttcc tggacgacga
     1561 tcagctcgtc atcggtccat tttcgcggag cgtcctgttg cactcccagt tgtgccacca
     1621 gccggccacc ttttatcagc ggtactccga cgaacgcatg gatgccggcg gcaatggatc
     1681 cttctttttc ggcatccgtc aaccccggtg tatcctccac attatcgatc accggcatct
     1741 cacctcgcag gaaccaccgg atcaccgtcg ggaaggcgtt gccggagaac ctgccgacac
     1801 gggggctata tcccgggctg acataattat cggggatcgt ataggtacta ccatcgggtt
     1861 cgaactccgc atagaaagca cggcttgcgt cgagatgctc tccaacgacc cggatcgccg
     1921 ttcgccggat ctcgacaggg tcggacagca ggtgcagcgc atcggtcaat ttgagcaggt
     1981 attcctgctg gcgctcgcgt cgtttgcgtt cggtaatatc attgaaaaaa acggctacct
     2041 gacaggtttc cggccggtcg aacgggatgg caaatacttc ataccaaacc ccgccggcaa
     2101 gatgcgccgc ggcgttctca aaatggatcg actcgccggt ttttactacg tcgccataga
     2161 tccggaacca atgctcttcc tgcgcaggct gcagttcctt gatcgtcctg cccacggcat
     2221 cgtttaaacc ggttatcttt tcaaaaacgg gattcacctc caggaaccgg taatcgtaag
     2281 ccttaccttt ttcatcgaag atcacctcga tgatacagaa tccctggttc atgttattaa
     2341 agagcgtgag atatttttcg gttgcctgtc tcgtggcttt gcgcaactca ctttccaaac
     2401 ccatgcccat aaattacact gttagataac aaatttcgct actttgaagg ataaatcgga
     2461 ttatttattt tcattatatt taataccgaa ttaaccttaa cacgaaaaac attctattct
     2521 tcgcactgcc ttttaccgtt tgcattggcc gggcacaaaa tggaaagatg atcgaaagga
     2581 ctccctacga actggcggac acaactattc agcgactata agatcgttta tctatccgaa
     2641 ggattaaaag tgaaaggtct gatggctgtc cccaaaaagg acggcaagta cccctgtctc
     2701 atttttaacc gcggaggtaa ccgggaactc ggcgtcctca atgatgtgca gatggttcgt
     2761 ctgctgggtg ggcccgccag ttggggatat atcacaattg ccagccaata ccgtggtaat
     2821 gccggcggtg aaggtcagga ggaatttggt ggcaaggacg tcggcgatgt tctgaatctc
     2881 attccgtctt tcgctgaaat tgccggtgcc gacacaacgc gtatcggggg cttcgggtat
     2941 ggcggatgct tttactacca ttcaacgacg gcctctgatg gtcaatgcat atcaacagct
     3001 gg
//
LOCUS       DASYSV010000272         4063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_822416, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000272 DASYSV010000000
VERSION     DASYSV010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822416"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VGQ51_08325"
     CDS             complement(<1..308)
                     /locus_tag="VGQ51_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9036611.1"
                     /translation="MHYVSVEELEKSYGVKPLFRNISFHIEEGDKIALVARNGSGKST
                     LLKILSGKDTPDDGTVWIHKDVTVALFEQEPTFMEDRSILDNIFHHEHPIINAIKDYE
                     "
     gene            complement(325..858)
                     /locus_tag="VGQ51_08330"
     CDS             complement(325..858)
                     /locus_tag="VGQ51_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV9036612.1"
                     /translation="MATTNWILDPSHSEVEFKVKHMMISNVSGRFTKFNATLETEGED
                     FMTAKVTFTADIDSITTGSDQRDGHLKSPDFFDAAKFPQIKFSTTKYENVDNDGSYEV
                     YGELTIHGVTKTVRFDTEFGGVIKDPWGNTRAGITVSGKINRKDFGLTWNGVTEAGNL
                     IVSEDVRVHVGLEFVKG"
     gene            complement(910..3621)
                     /locus_tag="VGQ51_08335"
     CDS             complement(910..3621)
                     /locus_tag="VGQ51_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEV9036613.1"
                     /translation="MRITMPFLLALLLSAPSAFSQLGVSALKTENLSDPIGIDSRQPR
                     FSWQLTAPSQDRDVLQTAYEIRVSRRPEGKDAVWNSGRIASQQSTQVLYDGPALREEE
                     NYCWQVRVWDNKGRHSSWSTTATWQMGPLKPADWKAAWIQPALPDTIACPLLRTTFTA
                     KGSIASAIVFITAHGLYEAQLNGHRIGDAVFTPGWTSYNHRLQYQAYDVTGLITPGKN
                     AIGALLGNGWYSGYIGYDWQHDVYGSKLGLLLQLVIRYKNGQTETIVSGPGWKSSTGE
                     VLHSEIYNGETIDHRKEKKDWSLADYDDKGWNNADTAVFPMDNLVATVNEPVRRHETF
                     HAVKLLTTPKGEHVIDFGQNLTGWVVVKIKGRSGDSVRLSHAEVLDKEGNFYTDNLRG
                     AKAQDLFVLRGGEEEVFEPHFTFHGFRYIRIEGWEGRIDPDDFTAVALYSDMPKTGEF
                     TCSNAMINQLQHNIQWGQQGNFLDIPTDCPQRDERLGWTGDAQVFSRTAAFNRGTENF
                     FSKWLKDLAADQLPGGSVPFVIPNVTGTGTSAGWADVATIIPWNLYLVYGDTVVLRTQ
                     YPSMKAWVEYIRHQAKDNLWNTGHHFGDWLFYRPFDDNDGRSAVTDKYLIAQCFYAHS
                     TQLLIDAARVLGKKDDVEAYSTLLQKIKDAFLREYMTPDGRLVSGTQTAYVLALQFDM
                     LPESLRKQAAERLIKNIQDYNYHLTTGFLGTPYLCHVLSRYGYTRVAYRLLLQDTYPS
                     WLYPVKMGATTIWERWDGIRPDGSFETPGMNSFNHYSYGAIGDWMYRVIAGIDTYEDG
                     PGYRHSSIAPQPGGGLDSAGADLLTGYGRLSSHWRLTNDSLSMDIIVPVNTTSTVTIP
                     APAGGMITEGGRKLADVKDISIESVGEGFLAVRIGSGTYHFALKVLN"
     gene            3693..>4063
                     /locus_tag="VGQ51_08340"
     CDS             3693..>4063
                     /locus_tag="VGQ51_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M19"
                     /protein_id="HEV9036614.1"
                     /translation="MFTIDAHLDLAMNALEWNRDLTLPVAAIREREAGLTDKPDRGRG
                     TVSLPALRGGDIGLVVATQIARYVAPDNPLSGWHSPWQAWAQTQGQLAWYKAMEEAGE
                     MVMIKDLPTLEKHVRTWMREGT"
BASE COUNT          844 a         1024 c         1221 g          974 t
ORIGIN      
        1 tcttcatagt ccttgatggc gttgatgata ggatgttcgt ggtggaagat gttgtctaag
       61 atggagcggt cttccataaa ggtcggttcc tgttcgaaaa gcgctaccgt gacatcctta
      121 tgtatccaca cagtgccatc atcgggggta tctttacccg agagtatctt cagaagcgtg
      181 gattttccgc tgccgttgcg cgcaacgagg gcgatcttat cgccctcttc gatatggaaa
      241 ctgatgttgc gaaacagcgg cttgacgccg taagactttt caagctcttc caccgagaca
      301 taatgcatgc ggcaaatgta cgtcttatcc tttcacgaat tccaaaccga cgtggacgcg
      361 aacatcctcg ctgacgatga ggttgcctgc ttccgtcacg ccgttccagg tgagaccgaa
      421 gtccttgcgg ttgatcttac cgctgacggt gatgcctgcg cgggtgttac cccagggatc
      481 tttgatgacg ccgccgaatt cggtatcgaa gcggacggtt ttcgtcacac catggatggt
      541 cagttcgcca tatacctcat aggagccgtc gttgtcaacg ttctcatatt tggtggtcga
      601 gaattttatt tggggaaatt tcgccgcatc gaagaaatcg ggagatttca gatgaccatc
      661 gcgctggtcg gatccggtag tgatcgaatc gatgtccgcg gtgaaggtga cctttgccgt
      721 catgaagtct tcgccttcgg tctccagcgt agcgttgaat ttcgtgaatc tgccgctgac
      781 attggagatc atcatgtgtt ttaccttgaa ttccacttcc gagtgggaag gatcgagtat
      841 ccagttggtc gtagccatgg tgcaattatt ttaacaaagg taaacccaaa ccctgtgccc
      901 gccattaacc tagtttaaga ctttgagggc gaaatgatac gtgcctgaac ctatccgtac
      961 ggccaggaat ccttctccga cggattcgat ggagatatcc tttacgtcgg cgagtttcct
     1021 gccgccttcc gttatcatgc ctcctgcggg ggcggggatg gtgacggtcg atgtggtatt
     1081 gacagggacg ataatgtcca tgctgagcga atcattcgtc agccgccagt ggctgctgag
     1141 tcgtccataa ccggtgagaa gatcggcacc tgccgagtcg agacctccgc cgggttgcgg
     1201 ggcgatgctg ctatgccggt aaccggggcc gtcctcatag gtatcgatac cggctatgac
     1261 ccggtacatc cagtctccga tggcgccata ggaatagtgg ttaaaggaat tcatgccggg
     1321 tgtttcgaag ctaccgtcgg ggcggatacc atcccagcgt tcccagatgg tggtggcgcc
     1381 catttttacg ggataaagcc aggacggata agtatcctgc agcaggaggc ggtaggcgac
     1441 ccgtgtatag ccataacggc tgagtacgtg gcatagatag ggtgtcccga gaaaaccggt
     1501 cgtaaggtga tagttgtaat cctgtatatt ctttataagc cgttcggctg cctgttttct
     1561 taaggattcc ggcaacatat cgaactgcag cgcgagtaca taggccgttt gggtaccgga
     1621 gaccaggcgg ccgtcggggg tcatgtattc gcggagaaag gcatctttga tcttttgcaa
     1681 cagcgtacta taggcctcta cgtcgtcctt tttgccgagg acgcgggccg catcgatcag
     1741 taattgggtg gagtgagcat agaaacattg agcgatgagg tatttatccg tgacggccga
     1801 gcgcccgtca ttgtcatcga aggggcgata gaaaagccag tcgccaaagt gatgaccggt
     1861 attccagaga ttgtctttag cctgatggcg gatgtattcc acccaggcct tcatgctggg
     1921 gtattgcgtt cttagtacga cggtatcacc atagacgaga taaaggttcc aggggatgat
     1981 ggtggcgacg tcggcccagc cggcgctggt gcctgtaccc gtcacgttcg ggatgacgaa
     2041 gggcacggag ccgccgggaa gttggtccgc agccaggtct ttcagccatt tggagaaaaa
     2101 gttctccgta ccgcggttga aggcggcggt tcgtgaaaag acctgtgcat cgccggtcca
     2161 tccgagtctt tcgtcgcgct gcggacagtc ggtggggata tcgagaaaat taccttgctg
     2221 gccccattga atgttatgct gcagctgatt gatcatggcg ttggaacagg tgaattcgcc
     2281 ggtcttcggc atatccgagt agagtgcaac ggcggtgaaa tcgtcggggt cgattcggcc
     2341 ctcccatcct tcgatgcgga tatagcggaa accatggaat gtgaaatggg gttcgaagac
     2401 ttcctcctct cctccccgga ggacaaagag gtcctgcgcc tttgcgcccc gcagattgtc
     2461 cgtatagaaa ttgccctctt tgtcgagcac ttcggcgtgg gacagacgaa cgctgtcgcc
     2521 ggatcttcct tttattttca ccaccaccca gccggtgaga ttctgaccga aatcgatgac
     2581 atgttcgccc tttggcgtcg ttagcagttt gaccgcatgg aaggtctcat gtcttctgac
     2641 cggctcattc acggtggcca ccaggttatc catggggaag acagcggtat cggcgttatt
     2701 ccagccctta tcgtcatagt cggccagaga ccagtctttt ttctccttgc ggtgatcgat
     2761 ggtctcgccg ttatagatct cggaatgaag cacttcaccc gtggaagact tccagcccgg
     2821 accggacacg atggtttcgg tctgcccgtt cttatagcgt atgaccagct gtagcagcag
     2881 ccccagcttg ctgccgtaga catcgtgctg ccagtcgtaa ccgatatagc cgctgtacca
     2941 gccattgccg agcagagcgc cgatggcatt ttttccgggt gtgatcaacc ccgtgacatc
     3001 ataggcctgg tattgcagcc ggtggttata ggaagtccat ccgggagtaa atactgcatc
     3061 gccgatgcgg tgaccgttga gctgcgcctc atagaggccg tgagcggtta taaagacgat
     3121 agcggaggcg atcgagcctt ttgctgtgaa ggttgtgcgg aggaggggac aggcgatggt
     3181 atccggtaat gccggttgga tccatgcggc cttccagtct gcgggtttca gcggacccat
     3241 ttgccatgta gccgtcgtac tccaggaaga atgacgtcct ttattatccc agaccctgac
     3301 ctgccagcaa tagttttctt cttcgcggag ggcggggccg tcatagagca cctgtgtgga
     3361 ctgttggctg gcgatcctgc cggaattcca gaccgcgtct ttgccttccg ggcgtcggct
     3421 gacgcggatc tcgtaggctg tctggaggac gtcgcgatcc tgggaagggg cggtcagctg
     3481 ccagctgaaa cggggctgcc ggctgtcgat accgatggga tcggagagat tctcggtctt
     3541 cagtgcggaa acgcccagct gcgagaatgc ggagggcgcg gatagcaaca gcgccaagag
     3601 gaacggcata gttattctca tgattgagga ggttgtgatg acaaatatag ggtaccgggg
     3661 gctttcggca gggggtttgt atatttgatc atatgtttac catcgacgca cacctcgatc
     3721 tggctatgaa tgcgctggaa tggaaccgcg atctcaccct gcctgtagcg gccatccgcg
     3781 agcgcgaggc ggggttgacc gataaacccg acaggggcag ggggacggtg tccttgccgg
     3841 cactgagagg tggtgacata gggttggtgg ttgcgaccca gatcgcacgt tatgtcgcgc
     3901 cggacaatcc cttatccgga tggcactccc cctggcaggc atgggcacag acgcagggcc
     3961 agctggcgtg gtacaaggcg atggaggaag ccggcgaaat ggtgatgatc aaggatctgc
     4021 ccactcttga aaagcacgtc cggacatgga tgcgtgaagg gac
//
LOCUS       DASYSV010000273         4452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_51600, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000273 DASYSV010000000
VERSION     DASYSV010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51600"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VGQ51_08345"
     CDS             complement(<1..940)
                     /locus_tag="VGQ51_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEV9036615.1"
                     /translation="MEIIHVSAECYPVAKAGGLGDVVGALPKYQRDLGHIAKVVMPMY
                     RTKFLRENEWEVVHKGYTHLHDYWFNYTVIKEKTNKLGFDLYLVDIFGLLDREKVYGY
                     GDDTERFTAFQIAVVDWIAAWQHFPDVIHVHDHHTALIPFMLKYCYAYQQLQNVPTVL
                     TIHNAQYQGQTGMDKTVYLPDWDIWKRGILEWAGALNPLACGIRCAWKVTTVSPTYLE
                     EMRHSAAGLEALLEYEKGKCSGILNGIDSVIWNPASDKYIQDHYDDETVEEGKARNKA
                     RLCERYNLDPKKPLITFIGRLVADKGADLLPQAIEDS"
     gene            1063..2718
                     /locus_tag="VGQ51_08350"
     CDS             1063..2718
                     /locus_tag="VGQ51_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9036616.1"
                     /translation="MNTTNQTTDIRTGAAYAAKQPTNTGALATLVLVFFFWGFIAASQ
                     SIFIPFCKHNFSLTQFESQLLGSAFYGAYFIGSLALYLISSLLKYDILNRIGYKKGII
                     YGIWISVAGALLIIPFANAHSFAGLLAAYFVIALGFSLQQTAAQPFAIMLGDPATGSH
                     RLNLAGGVNSLGTTLGPIIFSFFLFGSATGKGDEATVTNINNLFLIVACVFAAVALFF
                     TFSRLPAGKDDDKFEGSYKASRSLVLMTLLIVGVIVVGQLTSVEKLPLLIVTLVVVVG
                     ILFLSNGSAMGNATGWGAMRYPQLIYGMLGIFLYVGVEVTIDNNFGALLREPGYLTVS
                     GLDESKISKYVSLYWASLMIGRWTGAIGVFNLSEMGKRIATIVVPFIALAVALFVNHL
                     YSGDVSDLYPYACFVAVAVAAFFFGQQKPVKTLLTLACLAGIFILVGVLSKGIFSVYC
                     IMAAGLCNSIMWPCIFSLGIAGLGKFTSQGSAFLIMMILGGAVIPPFQGAVGDVLGSA
                     TGMHYSYLVAVGCYLLLAWLALKLKSTLQKQGLNFDEQIGGGH"
     gene            2858..3841
                     /locus_tag="VGQ51_08355"
     CDS             2858..3841
                     /locus_tag="VGQ51_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV9036617.1"
                     /translation="MATASSHSFDPLAIGIDIGGTGTKFGIVDRNGNVLFSSEISTRG
                     HSQVEGFIDELHRHLSVLIEKAGGIGRMRGIGVGAPNGNYYSGTVEYAPNLPWRGIIP
                     LANLIQNKFQLPAVLTNDANAAAIGEQMYGAAKGMKDFIMITLGTGVGSGIVANGKLI
                     YGHDGFAGELGHTIIIPNGRHHDGTGKNGSLESYASATGVKLTAIEMLANSKEPSLLR
                     KVPKDKLDSRQVYDAAIEGDKLAKDIFDFTGSVLGLALANFVMFSSPEAIILFGGLTK
                     AGDLILKPTRENMENNLIQVFQNKVKILVSHLKESDAAILGASALVWDMKN"
     gene            complement(3864..>4452)
                     /locus_tag="VGQ51_08360"
     CDS             complement(3864..>4452)
                     /locus_tag="VGQ51_08360"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEV9036618.1"
                     /translation="LRGTGIDSQLHYYPTRGAIGIDHKRRADIAWTFTDSSRRWPYAF
                     EEMPVIAKGADTVPSIYDLNDIEWKWWKMRTAVGGGPVLIHDGKIKITEREEQLYPDS
                     RRDGEHAPRTAMGYTRDGRLIVLVIQGRTPGVAAGASLQQEAEILLGLGCFEALNLDG
                     GGSSCLLVNGKETIRPSDREGQRAVPAVFVIQPRH"
BASE COUNT          946 a         1213 c         1199 g         1094 t
ORIGIN      
        1 aggagtcttc gatcgcctga ggcagcagat cggccccttt atcggcgacc agtcgtccga
       61 taaaagtgat caacggtttt ttcggatcga ggttatagcg ctcgcaaagc cgggccttgt
      121 tcctggcctt gccttcctcc acggtctcat cgtcataatg atcctgtatg tatttgtccg
      181 aagcgggatt ccagatcacg gagtcgatcc cgttgaggat gccgctgcat tttcctttct
      241 catattccag cagggcctcg agacccgcgg cggaatggcg catctcttcc aggtaggtag
      301 ggctcacggt ggtgaccttc caggcacacc ggatcccgca ggccaggggg ttgagcgcac
      361 ctgcccattc cagtatcccg cgtttccaga tatcccaatc gggaagataa acggtcttat
      421 ccatacctgt ctgaccctgg tattgcgcat tgtggatagt caataccgtt ggtacgttct
      481 gcaattgctg ataggcatag caatatttga gcatgaaggg gatcagagcg gtatggtggt
      541 cgtgcacgtg aatgacgtcc ggaaaatgct gccatgccgc gatccagtcg acaacggcga
      601 tctggaaggc tgtaaacctt tccgtatcat ccccataacc atagaccttt tccctgtcga
      661 gcaacccgaa gatatccacg agataaagat cgaaaccgag cttattggtc ttttccttga
      721 tgaccgtata attgaaccag tagtcgtgga gatgggtata gcccttatgg acgacttccc
      781 attcgttctc ccggaggaat ttggtccggt acatcggcat gaccaccttg gcgatatgtc
      841 cgaggtccct ctggtatttg ggtaatgccc cgaccacgtc cccaagaccg ccggccttgg
      901 ctacgggata acactccgcc gaaacgtgga tgatttccat taaggatcaa tttagtaagg
      961 ttttcggtta aacaggctac ctaagatgaa aaaatttttc gcttcataaa aacttggtat
     1021 tttggacttt tattcccatt aaccgcttaa ctgcgtcgcg acatgaacac tacaaatcaa
     1081 accaccgaca tccgaaccgg agccgcttat gcggcaaaac aaccaactaa taccggtgcg
     1141 ctcgctactt tggtcctcgt cttttttttc tggggattca ttgccgcctc gcaaagcatc
     1201 ttcatccctt tctgtaaaca taatttcagc ctgacccaat tcgaatcgca gctgcttggt
     1261 tcggcattct atggcgccta tttcatcggg tcgctggcgc tgtatctcat atcaagtctt
     1321 ctcaaatacg atattttaaa ccgcatcggg tataaaaaag ggatcattta cgggatctgg
     1381 atatccgtag cgggtgctct attgatcatt cctttcgcca atgctcactc ttttgcgggg
     1441 ctgcttgctg cctattttgt gattgcgctt gggttttcgc tccagcaaac ggctgcgcag
     1501 ccatttgcga tcatgctggg ggatccggca acgggtagtc accgactgaa cctggccggc
     1561 ggggtcaact cgctcgggac gacgctggga ccgatcattt tcagcttctt tttgttcggg
     1621 tcggcgacag gcaagggtga tgaggcaacg gttacgaata tcaacaacct gttcctgatc
     1681 gtagcttgtg tattcgccgc cgtggcgttg ttctttacct tttccaggct tcccgcaggc
     1741 aaggacgacg acaaattcga agggtcctat aaagcatcga gatcattggt ccttatgacc
     1801 ctgttgatcg tgggcgtcat cgtggtcggt cagctgacgt ctgtcgaaaa gctgccattg
     1861 ctgatcgtca cgctggtggt ggtcgtggga attcttttcc tgtcgaatgg aagtgccatg
     1921 ggcaatgcca cgggttgggg cgcgatgcgc tatcctcagc tgatctatgg catgctaggt
     1981 atttttctat atgtgggagt agaggtaacg atagataaca atttcggcgc cctgttgcgc
     2041 gagccgggat atctcacggt atccgggttg gacgaaagca agatctccaa atatgtgagc
     2101 ttatattggg caagcctgat gatcggccgg tggaccggcg ccatcggggt attcaacttg
     2161 tcggagatgg gaaaacgtat cgcgaccatc gtggtccctt ttatagcgct cgccgtggcg
     2221 ctgtttgtca atcatcttta tagcggtgat gtcagcgatc tttatcccta tgcctgtttc
     2281 gtcgcggtgg ccgtggccgc ctttttcttc ggtcagcaaa agcccgtcaa gaccctgctc
     2341 acgctggcgt gtctggccgg tattttcatc ctggtcggtg tgctctccaa gggtatcttc
     2401 tcggtctatt gcatcatggc cgcgggtctt tgcaactcga tcatgtggcc ctgtatcttc
     2461 tcgctcggca tcgccggtct cggaaaattc accagccagg gctcggcctt cctgatcatg
     2521 atgatcctgg gtggcgcggt gatcccgcct ttccagggcg ccgtgggcga tgtgctgggt
     2581 tcggcaaccg gtatgcatta tagctatctc gtagcggtgg gttgctacct gctgctcgca
     2641 tggctggcgc ttaaactgaa gagcacgctt cagaaacagg ggctgaactt cgacgaacag
     2701 atcggcggag gtcattagga atacattaaa taatgccgcg ccggcggagg cggggttgcc
     2761 gatccataaa tcggactatc tttgtgaccc tggagccgcc tgtggcggtt cgtccgcaag
     2821 gacaatcatg agcctcaaaa caaactacac atcacacatg gcaacagcca gttctcattc
     2881 ctttgacccg ttggctatcg gaatcgatat cggcggtaca ggcacgaaat tcgggatcgt
     2941 cgaccgcaac ggaaatgttc ttttttcaag cgagatctct acacgcgggc acagccaggt
     3001 ggaagggttc atcgatgaat tgcatcgcca tctgtccgta ctaatcgaaa aagccggcgg
     3061 catcgggagg atgagaggca tcggcgtcgg tgctcccaat ggcaattatt acagcggaac
     3121 ggttgaatat gcaccgaacc ttccctggcg aggcatcatc ccgctggcca atctcatcca
     3181 gaataaattc cagcttccgg cagtgctgac caacgacgcc aatgccgcgg ccatcggcga
     3241 acagatgtat ggcgccgcca agggcatgaa ggattttata atgatcacgc tcgggacggg
     3301 agtcggcagc ggcatcgtcg ccaatggcaa gctcatttat ggccacgacg gctttgccgg
     3361 tgaattaggt cacaccatca tcatccccaa cggccggcat catgacggca ccgggaaaaa
     3421 tggttctctc gagagctatg cctcggccac cggtgttaag cttacggcca tagagatgct
     3481 cgcgaacagc aaagagccca gcctcctgcg aaaggttccg aaagacaaat tggattcgag
     3541 acaggtgtac gacgccgcca tcgaaggaga caagctggcc aaggatatct tcgattttac
     3601 cggctctgtg ctgggtctgg cgctggccaa tttcgtcatg ttctcgagcc cggaagccat
     3661 catccttttc ggcgggctca ccaaggccgg cgatctgatc ctgaaaccta cccgggagaa
     3721 tatggaaaac aatctcatcc aggtcttcca aaacaaggtg aagatcctcg tcagccattt
     3781 gaaggaatcc gatgcggcca tcttaggcgc cagcgcattg gtatgggata tgaagaatta
     3841 gtgttgcggc ccggcgctcg tcattagtgt ctcggctgga tcacgaaaac cgctggtact
     3901 gcgcgttgtc cctcgcggtc cgatggccgg atcgtctctt tgccattgac caacaggcag
     3961 ctgctgcctc ccccatccag gttcagcgcc tcaaaacaac ccaggccgag caggatctcc
     4021 gcctcctgct gcagcgaggc gcccgcagcg acacccgggg tcctgccctg tatgaccagc
     4081 acgatcagac gaccgtcccg ggtatagccc atcgccgtcc gcggtgcatg ttcgccatcc
     4141 cgtcggctgt cgggataaag ctgctcctcc cgttcggtga tctttatctt cccgtcatgg
     4201 atgagtacgg gacctccacc gacggccgtc cgcatcttcc accatttcca ttcaatatca
     4261 ttcagatcgt aaatcgaggg tacagtgtcc gcgcccttgg cgatcaccgg catttcctcg
     4321 aatgcatatg gccagcgacg cgagctgtcg gtaaatgtcc aggcgatgtc agcgcggcgt
     4381 ttgtggtcga tgccgatggc gccgcgtgtg ggataatagt gcaattgtga gtcgataccc
     4441 gttccgcgaa gc
//
LOCUS       DASYSV010000274         7819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_564244, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000274 DASYSV010000000
VERSION     DASYSV010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564244"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VGQ51_08365"
     CDS             <1..909
                     /locus_tag="VGQ51_08365"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEV9036619.1"
                     /translation="EIPILPAFRPDNAMNVDEPGRFNNYIKKLEAITNVAISSFNDYL
                     FALENRHDFFASMGCTVSDHGLEEICVEEFTGWEVEAIFNKVRAGKELNETERRKFRS
                     AMLVHFAEWDWAKGWVQQFHLGALRNNNTRMLQKLGPDTGWDSIGDFSQSRTLARFLD
                     RLDKEDKLARTILYNLNPADNELIATMAGNFNDGSVAGKIQFGSAWWFLDQKDGMTRQ
                     INALSNMGLLSRFVGMLTDSRSFLSYPRHEYFRRILCNLFGEEITNGELPNDLEWIGK
                     VIRDICYYNARQYFNFQPAGSLAYTE"
     gene            996..1418
                     /locus_tag="VGQ51_08370"
     CDS             996..1418
                     /locus_tag="VGQ51_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036620.1"
                     /translation="MAKKDKKTIDMPDVQDIPGQEHIRPPRLGEMEDTTASSADEEGE
                     QTDDPDDIGFEDIEEREPGTEDDVLNEAALDDKDSDGDPLNEKSFGEDVSGDDLDVPG
                     ADADDEDEDIGEEDEENNEYSLSKNDEEHDDLERDAMD"
     gene            1584..2129
                     /locus_tag="VGQ51_08375"
     CDS             1584..2129
                     /locus_tag="VGQ51_08375"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV9036621.1"
                     /translation="MERTDGQLSAHSGTDKQHLLQQLFSDMLEDIYYAEKHLTKALPK
                     MSKACTTADLRQAFDEHLEQTEMHVARLERVFGLLGRKAKARKCEAIEGLTKEAEDLK
                     KETEKNTLTRDVALIAAGQKVEHYEIATYGTLVQLASNMHLNEVVSLLEETLKEEKAA
                     DLKLTNIAEHSVNWQALEAEG"
     gene            complement(2201..3364)
                     /locus_tag="VGQ51_08380"
     CDS             complement(2201..3364)
                     /locus_tag="VGQ51_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV9036622.1"
                     /translation="MKEIKFASPVRTAAILIILVICFIILSWAEDVIVPLLFAAIFSS
                     LLFPLCFRLEGWGCHKGVASFLAVLAAGVATAAFLGIVLMQVMHLAGQGPQFVTRLSQ
                     LLDHMENVIAQHLHVGKSTTADHVHLMLSKLLDNSSAYFSSTMSFTTNMISQFLLILL
                     FSFFLLYLRVFFLEFFYKAFASSEKTLIDETMQKIYTVIQNYLLGLLKVIAIIGTLNS
                     IGLWALGIESPLFFGFLGGILVIIPYIGILIGSALPIVVALVTKDSYWYAVGVMGVFL
                     FVHILEGNVITPYVVGSKVSINPLAAVFSLLLFGKLWGLSGLILALPVTAICKIIFDT
                     LPGFKAVGFLLGKPQKYHFKRHSILHAKLRAMHDASGGAVGAAADGVTDTATK"
     gene            complement(3460..3606)
                     /locus_tag="VGQ51_08385"
     CDS             complement(3460..3606)
                     /locus_tag="VGQ51_08385"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEV9036623.1"
                     /translation="MSGLLYLIAVILLIGWLLGVFVYSVGGLIHILIVLALISLVLGI
                     IRRA"
     gene            3793..4704
                     /locus_tag="VGQ51_08390"
     CDS             3793..4704
                     /locus_tag="VGQ51_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV9036624.1"
                     /translation="MRRFKLSLCWKAMKKAAVDFNNDNGFKLAASLSYSMIFSIAPLL
                     IVVISLAGVFWGRQAVEGRIYQQIKDLVGVSPAVQIQDIIRNIQQSRHSVTGAIIGGA
                     VLVIGATSVFTEIQGSINYMWCIQSKPKKGWLKFLMNRLLSFSLIVAFGFISMVSLVI
                     NSLMDVLSDDLKRFFSHFTVYLFYAVNFVLALAAVVLLFMVVFRILPDAIIKWSDAFV
                     GALFTGILFMLGKLIIGVYLGHSNITHLYGAAASIIVILTWVFYSSIILYYGAEFTRS
                     YAILSGGGIRPSETAVFIIKSESRELA"
     gene            complement(4701..5474)
                     /locus_tag="VGQ51_08395"
     CDS             complement(4701..5474)
                     /locus_tag="VGQ51_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEV9036625.1"
                     /translation="MRSIWTGAIGFGLVNIPIKIYSAVQGSELDLDMLDSKDHANIRF
                     KRVNENTGKEVSWDHIVKGYKLDDKYVVLTDEDFEKASPEKSKIIGINEFVDEKEIDG
                     IYYETPYYLEPDKSGGAAYILLRDALHKTGKVAFGSFVMRNKEGLCLIKPMEKILVLN
                     RIRWAQEVRSTEDIKIPGSAPKPAEIKMAIQLIDQLTGRFDIEKYKDTYSDELLKIIK
                     AKAKGTKAAKPTLRVVHSTKSKDLLSQLKASLGGKRKAS"
     gene            complement(5517..6941)
                     /gene="rpoN"
                     /locus_tag="VGQ51_08400"
     CDS             complement(5517..6941)
                     /gene="rpoN"
                     /locus_tag="VGQ51_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEV9036626.1"
                     /translation="MLKQVQTQKQQHTILPQQIELLNLFHLNTLELEQRIQDELSENP
                     ALEELASGDENSSDKCNKELVQDFQNWEEYGYDDIPDYKTEYNNYINTEKVPEKPIAE
                     IPDFRDELKKQCRFLEIPEDKYYLFDFLIDSLNENGFLEQDLDELASEISFKKKVWIE
                     PEELEAALHLIRELEPGGAGCRSVQEFMLLQLKRMHTRRPDVRMAISLLENHFSDLRS
                     CNLDKLRKQLGLEDDEIRIVLNLLAHLSTRPFSPSNNALQANPSILPDFIVIDEGDTL
                     ELALYRQRSSNLHISQSWMEMVQQTSQNASMDRSTKQYLRSKLSSAQWFINAIQQREN
                     NMLKIMRAILDMQYEYFKYGDILLLRPMKLKDIADKVKVDISTVSRLTCNKYAETPFG
                     TILLKDLFTEGIVNQEGTNISNKVIQNIISEVIEMEDKKAPYTDRQLVAILAGKGYSI
                     ARRTVAKYRELMNIPVAQVRGLWN"
     gene            7209..>7819
                     /locus_tag="VGQ51_08405"
     CDS             7209..>7819
                     /locus_tag="VGQ51_08405"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEV9036627.1"
                     /translation="MSAPASANPPHSSLPRRLARILLRTVLIIVVIVLVLILLVQMPF
                     VQDFARGKAERYLSRKLKTRVRIGHLNIAFFNSVTLKGVYMEDRRQDTLLSAGLIDVR
                     LQMLGLLHNRLNIDAITLQDLTAKVLRGGHDTVFNYQFIVDAFAPTPAAKPDTTGGEP
                     MAIRLRRLNLDRIRLVYRDTVTGTNATVYIGHDKTIVDSMDLN"
BASE COUNT         1893 a         2043 c         2028 g         1855 t
ORIGIN      
        1 gagataccca tcctgccggc ttttcgtccc gacaatgcga tgaatgtcga cgagcctggc
       61 cggttcaata actatatcaa aaagctggaa gccatcacca acgtcgccat atccagcttc
      121 aacgattatc ttttcgccct cgagaaccgg catgacttct ttgcctctat gggttgtacg
      181 gtcagcgatc acggattgga agagatctgc gtcgaagagt tcaccggctg ggaggtcgaa
      241 gctatcttca acaaggtaag ggcgggcaag gagctgaatg aaaccgagcg ccgcaaattc
      301 cggtcggcca tgctcgtcca tttcgccgaa tgggattggg cgaaaggatg ggtacagcaa
      361 tttcatctcg gcgcattgcg caacaataac actcgcatgt tgcaaaagct ggggcccgat
      421 accggttggg atagcatcgg ggatttttcg cagagccgta ccctggcgcg ttttctcgat
      481 cgtctcgata aagaggacaa actggcccgg accattctct ataatttaaa tcctgcggat
      541 aacgagctga tcgccaccat ggccggtaat ttcaatgacg gttccgtggc cggtaagatc
      601 caattcggct cggcatggtg gttcctggat caaaaggacg gaatgacccg gcagatcaat
      661 gcgctgtcga atatgggtct gctaagccgt tttgtaggaa tgctgaccga ctcgcgtagc
      721 tttctctcgt acccccggca tgagtatttt cgaaggatcc tttgcaatct tttcggagaa
      781 gagatcacta atggcgaact gcccaatgac ctggaatgga tcgggaaagt gatcagggat
      841 atctgttact ataacgcgcg gcaatatttc aatttccagc cggcgggatc gctggcgtac
      901 acggaataga ccgggcatcc gcctaccgcc tggccccgcc caggcggggc actcttattg
      961 ctgtattcct aactttgcat tacaaacttt cacctatggc aaagaaggac aaaaaaacga
     1021 tcgacatgcc cgatgtacag gatattcccg ggcaggagca tatccggccg ccccggctcg
     1081 gcgaaatgga ggacacgaca gcatcctccg ccgatgagga gggcgaacag accgacgacc
     1141 ccgatgacat tggattcgaa gatatcgaag agcgcgagcc cggcaccgag gacgacgtcc
     1201 tcaacgaagc cgccctcgac gacaaggatt cagacggcga tcccctcaat gaaaagagct
     1261 tcggggaaga cgtgtctggc gatgacctgg atgttccggg cgccgatgcc gacgatgaag
     1321 acgaagatat cggggaggaa gacgaggaga ataacgagta cagcctgagc aagaatgacg
     1381 aggagcacga cgatctcgag agggatgcga tggactaggt cccgtcgggc gttggcgccg
     1441 ggtgcacggg aatcgcagag tgaggaataa cgacatggga atgaccgcag tacggggaaa
     1501 atttggcaca attaccgccc ggctgaccca aaatagccgt ggcacatctt tttctggata
     1561 atatgtaacc aaatgtgcag gacatggaaa ggaccgacgg acagttatcc gcgcattccg
     1621 gaaccgacaa gcagcatctg ttgcagcaac ttttttccga catgctggag gatatctact
     1681 acgccgagaa gcatttgacg aaggcccttc ccaaaatgag taaggcctgc acgaccgccg
     1741 acctccgcca ggcttttgac gaacatcttg aacagaccga aatgcatgtc gctcgtctgg
     1801 aaagggtttt cggtctgctg ggcaggaagg cgaaggccag gaaatgcgag gcgatagaag
     1861 ggttgacaaa ggaggcggaa gacctgaaga aggagacgga aaaaaatacc ctgacccggg
     1921 atgtggcgct catcgccgcc ggccagaaag tggagcatta tgagatcgcc acctatggca
     1981 ccctggtcca gctggcaagc aatatgcatt tgaacgaagt ggtctccctg ctggaggaaa
     2041 ctctgaaaga ggaaaaggct gccgatctca agctgacgaa tatagccgag cacagcgtca
     2101 attggcaggc gctggaagcc gaaggttaaa aaatagtata aactttttgt gcgatagagg
     2161 tttaggttaa tggacgccgc cccggttcac cggggcggcc ttattttgtc gcggtgtcag
     2221 tgacgccatc tgcggcggcg ccaacggcgc cgccgcttgc atcatgcatc gcacgcagct
     2281 tggcatggag aatagaatgc cgtttgaaat ggtatttctg cggtttgcca agcaggaacc
     2341 ccaccgcctt gaaaccgggc agcgtgtcga agatgatctt gcagatagcc gtcacgggta
     2401 gcgccagtat cagacccgac aacccccaca gcttcccgaa aagcagcagc gagaaaacgg
     2461 ccgccaacgg gttgatgctc accttcgagc ccactacata aggagtgatg acattccctt
     2521 cgaggatgtg tacgaacagg aaaacgccca tgacgcccac cgcataccaa taggaatcct
     2581 tggtgaccag ggcgaccacg atcggaagcg ccgatccgat caggataccg atatatggta
     2641 tgatgaccag tattcctccc aaaaatccaa agaatagcgg cgactcgatc cccagcgccc
     2701 agagaccgat cgaattgagc gtgccgatga tcgcgatcac ctttagcaga cccagcaggt
     2761 agttctggat cacggtatag atcttttgca tcgtctcatc gataagcgtt ttttccgagg
     2821 aggcgaaagc cttatagaaa aattccagga agaagacgcg caggtataac aggaagaagc
     2881 tgaacagcag gatcagcagg aactgcgaga tcatattggt cgtgaacgac atagtagagg
     2941 agaaataggc gctgctattg tcgaggagct tgctcagcat taagtggaca tggtcggccg
     3001 tggtcgattt gccgacgtga agatgttgcg cgatgacatt ctccatatgg tccagcaact
     3061 gggaaagtcg cgtcacgaat tggggaccct gacccgcgag atgcatcacc tgcatgagga
     3121 cgatccccag gaatgccgct gtggccaccc ccgccgccag tacggcgagg aacgaagcca
     3181 cccccttatg acagccccag ccttcgagcc ggaaacacag cgggaaaagc agcgaggaaa
     3241 agatagccgc gaataacagg ggcacaatga cgtcctccgc ccacgaaaga atgataaaac
     3301 agatgaccag aatgatcagg atggcagcgg tacgtacagg ggatgcgaat tttatttctt
     3361 tcatagtcgt gggatggccg gttaagaata aaaaaatagc cggtcattcg accggctccg
     3421 ggaccgcgcg cgtggtccca taaccccgaa acatgccggt caggccctac ggataatacc
     3481 caggacgagg gatataagcg ccagtacgat taatatatgg atcaatccac ctacactgta
     3541 cacgaaaacc cccagtagcc atccgatcaa caagataact gcgatcaaat acaataagcc
     3601 gctcataatt caatttttga ttattaatgt gggatttgat ttgcctcatc cttcatattg
     3661 aaaaatgctt gccagatttt gttaaggctt ggaaggccac tataaaacaa catgataagg
     3721 gcaggagtgg ggaaaaaaat ccacattaac acccgttacc ggattggcat ggtcttgccg
     3781 ctatcccgta caatgagacg gttcaaactt tccctctgct ggaaagccat gaaaaaagcg
     3841 gcggtcgact tcaacaacga caacggcttc aaactggctg cttcgctttc ctattccatg
     3901 atattttcca tcgcccccct cctgatcgtg gtcatttcac tggccggcgt cttctggggc
     3961 cggcaggcgg tggaaggccg catttaccaa cagataaagg acctggtggg agtgagccct
     4021 gccgtacaga tccaggacat tatccgcaat atccagcaat ccaggcattc ggtcaccggc
     4081 gccatcatcg gcggggccgt cctcgtcatc ggcgccacca gcgtttttac cgagatccag
     4141 ggctcgatca actatatgtg gtgcatccag tccaaaccga aaaaaggctg gctcaaattc
     4201 ctgatgaacc gtctcctctc attctcgctt atcgtagcct tcggctttat atccatggtc
     4261 tcgctggtga tcaattcact gatggatgtg ctgagcgacg atctgaaaag attcttcagc
     4321 catttcaccg tctatttgtt ctatgccgtc aattttgtac ttgccctcgc ggcggtcgta
     4381 cttttgttca tggtggtttt ccgtatcctg cccgatgcga tcattaaatg gagcgatgca
     4441 ttcgtcggcg ccctttttac aggaatatta tttatgctgg ggaaattgat catcggtgtc
     4501 tacctggggc attccaatat tacccatctg tatggcgcag ccgcttccat catcgtcatc
     4561 ctcacctggg tattttattc ctccatcatc ctttactatg gcgcggagtt tacgcggtcc
     4621 tacgctatcc tcagcggcgg cgggatccgc ccgtccgaga cggccgtatt cattataaaa
     4681 agtgaatcga gagaactcgc ttagctggcc ttccgttttc ctcccagact ggccttcagc
     4741 tgagacaata gatccttact cttggttgag tgtacgaccc gcagagtcgg tttggcggct
     4801 ttggtcccct ttgccttcgc tttgatgatc ttcagcaatt catctgaata agtatccttg
     4861 tatttttcga tatcgaaacg gcccgtcagc tgatcgatca gctggatagc catctttatc
     4921 tcagccggtt tcggcgccga gcccgggatc ttaatatctt ctgtggaccg gacctcctgc
     4981 gcccagcgga tccggttcag cacgaggatt ttttccatcg gcttgatcag acagagccct
     5041 tccttattgc gcatgacaaa gcttccgaaa gccaccttcc cggtcttatg cagagcatcc
     5101 cgtaacagga tataggccgc cccccccgat ttgtccggct ccaggtaata aggcgtctcg
     5161 taatagatgc cgtcgatctc cttctcatcg acgaattcat tgatgccgat gatcttcgat
     5221 ttttcgggac tggccttttc aaaatcctcg tccgtcagca cgacatattt gtcatccagc
     5281 ttgtaaccct ttacgatgtg gtcccaggaa acctctttgc cggtgttctc gttgacgcgt
     5341 ttgaaccgga tattcgcgtg atcctttgaa tcgagcatat ccagatcgag ctcgctgccc
     5401 tggacggcac tgtagatctt aatggggata ttcaccagtc cgaaaccgat ggcccctgtc
     5461 catatcgaac gcatagacgt tttttttcaa atggagatca aacttcctgc cggagattaa
     5521 ttccacaacc cgcgcacctg ggccacaggg atattcatca gctccctgta cttcgccacg
     5581 gtccggcggg cgatggagta tcccttcccc gccaggatgg ccaccagctg acggtccgta
     5641 taaggagcct tcttatcttc catttcgatg acttcgctga tgatattctg gatcacctta
     5701 ttgctgatat tggtgccttc ctggttgacg ataccctccg taaaaaggtc tttcagcagg
     5761 atggtgccaa aaggcgtctc ggcgtatttg ttacaagtga gccggctgac cgtggagata
     5821 tcgaccttta ccttgtcggc aatatccttc agtttcatag ggcgcagcag caggatatct
     5881 ccgtatttga aatattcgta ttgcatgtcc aaaatggccc gcatgatctt gagcatattg
     5941 ttctcccgct gttgtatcgc attgatgaac cattgcgcag aagacagctt gctccgcagg
     6001 tattgcttgg tagaccggtc catggacgca ttctgggagg tttgctgtac catttccatc
     6061 caggactgac tgatgtgcag attgctgctc cgctgacgat acagggcaag ttccagtgta
     6121 tccccttcat cgatgactat gaaatccggc aggatcgacg ggttggcctg aagggcgttg
     6181 ttggaggggc tgaacgggcg ggtgcttaaa tgtgccagca ggttcagcac gatgcggatc
     6241 tcgtcgtctt ccagaccgag ctgtttccgc agtttgtcca ggttgcagct gcgcagatcg
     6301 gagaaatgat tttcgagcag actgatggcc atccgcacat ccggccgcct ggtatgcatc
     6361 cttttcagct gcagcagcat gaattcctgg accgaccggc agccagcccc gccaggttcc
     6421 agctccctga tcaggtggag tgccgcttca agctcctccg gctcgatcca gacctttttt
     6481 ttgaaagaga tctcagaggc aagctcatcg aggtcctgtt ccaggaaccc gttctcgttg
     6541 agcgaatcga tgagaaaatc gaagaggtag tatttatctt ccggaatttc cagaaaacgg
     6601 cattgttttt tcaattcatc cctgaaatcg ggaatttcgg ctatcggctt ttccggaacc
     6661 ttttcggtat tgatgtagtt gttgtattcc gttttatagt ccggtatgtc gtcgtatcca
     6721 tattcttccc aattttggaa atcctgcacc aattccttgt tgcatttatc cgaagagttc
     6781 tcgtcacccg aggccagttc ttccagggcg gggttttcgc tcagttcgtc ttgaatgcgt
     6841 tgctcgagtt ccagggtatt gaggtggaat agatttagta gttctatttg ttgcggaagg
     6901 atagtgtgtt gctgtttctg cgtttgtacc tgtttcagca tggtcacctt ttttagtacg
     6961 gtaccgtagg acaaaacaga cgccagaatg gtaaacgctt tggattgaag catgaacacg
     7021 cctgcctacc caaaaggtgt agaaatctcg gtacgcatat gtggaaaaaa gagcccaaat
     7081 tcatgaatga caagactttc taatacgagt agtcaaacat cgcactaagg gtctatatgt
     7141 agaagatggt ccgcattttg tcgggaacac ttattgtcct aatttaatat tattgagaat
     7201 tttaatttat gagcgcacca gcatccgcaa atccgcccca ttcctcactg ccacggcggt
     7261 tagcgcgcat tcttttacgc acggttctta tcatcgtcgt catcgtgctg gtcctcatcc
     7321 tgctggtgca aatgcctttt gttcaagact ttgcccgcgg gaaagcggag cggtatctat
     7381 cccgtaagct caagacgcgc gtccggatcg gacatctgaa catagcgttt tttaattcgg
     7441 tgacgctgaa aggggtttac atggaagacc gccggcagga tacccttttg tcggcagggc
     7501 tcatcgacgt ccgtctgcaa atgctgggct tgctgcacaa ccggctcaat atcgatgcta
     7561 taacgctgca ggatcttacc gctaaagtct tacgcggtgg tcacgatacc gtattcaact
     7621 accagttcat cgtggacgcc tttgccccga caccggccgc caaaccggat actaccggag
     7681 gagaacccat ggcgatcaga ttgcgtcgcc tgaacctcga ccggatccgg ctggtctacc
     7741 gcgacacggt caccggcacc aatgctacgg tttacatcgg ccatgacaag accatcgtcg
     7801 acagtatgga cctgaacca
//
LOCUS       DASYSV010000275         7956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1714015, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000275 DASYSV010000000
VERSION     DASYSV010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714015"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VGQ51_08410"
     CDS             <1..930
                     /locus_tag="VGQ51_08410"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV9036628.1"
                     /translation="TPLVLISNIYIIAFYYLNTYLFLPRLFDHKRFVSYTVLILALLV
                     FYGFLTRIYHGVFGYPTLPQNTVFTRAQNPRARPLINSGNLALFLLVFVFSTGIRVIN
                     QWLRSEQRNKEIANEKLKAELSFLKAQINPHFLFNTLNNIYALASAQSENTAPAVMKL
                     SSIMRYVLTEARNDLVPLEKEIQFTSHYIELQKMRLTDKAVVDFTVRGEPFGRQIAPL
                     LLLPFVENAFKYGISTRERSPIAILLEIKKETFEVSICNHKYFNTMLRASDNTGIGIN
                     NTRRRLDLFYEDRYKLDIDDKPNEFSVHLKIPV"
     gene            930..1628
                     /locus_tag="VGQ51_08415"
     CDS             930..1628
                     /locus_tag="VGQ51_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9036629.1"
                     /translation="MLTCIAIDDEPLALEVLKKYIAKIYFLDLKGVFTDPFEAQKVMD
                     SAPVDILFLDIQMPDINGIEFSRIINKKNTAVVFTTAFSEYAVDGFNVDAIDYLLKPI
                     EYDRFLKAVYKAKEYVDYLTSQELQEGYIFVKSDYQMVKVNLKEILYIEGLDDYIKIY
                     LPGKSILTLMTLKTIAQKLPAREFIRAHRSYIVPISKIEHISKSKIKIGDKEIPIGVS
                     YSESFFAAMENKLN"
     gene            1817..3340
                     /locus_tag="VGQ51_08420"
     CDS             1817..3340
                     /locus_tag="VGQ51_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV9036630.1"
                     /translation="MEFLQQLHIGRENAGTSTGTQWLPSTASVLESFSPVDGKRIATV
                     TTTDKAGYEAVVSAAQKAFAEWREWPAPRRGEVVRQIGEALREHKNDLGRLVSYEMGK
                     SLQEGYGEVQEMIDICDLAVGLSRQLNGLTMHSERPAHRMYEQWHPLGVVGIISAFNF
                     PVAVWSWNAMIAWVCGNTCVWKPSEKTPLCALACQHIVSTVFTANKVPEGVCGLVTGA
                     REPGEWLASDARIPLISATGSTRMGRAVGIAVATRLGRSILELGGNNAIVISSDADLN
                     IAIPGAVFGAVGTAGQRCTTTRRLIIQDAIYDKVKERLKTAYGQLRIGDPLDENNHVG
                     PLIDKDAVGLYRSAIDQAKQQGCRFIIDGEVLTGDGFESGCYVRPCIAEAKPDDPIVA
                     QETFAPILYLLRYGSLDEAIAIQNGVPQGLSSSIMTLNLREAERFLSAAGSDCGIANV
                     NIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINYSTQLPLAQGIKFNV"
     gene            3422..4843
                     /locus_tag="VGQ51_08425"
     CDS             3422..4843
                     /locus_tag="VGQ51_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9036631.1"
                     /translation="MRKWAPIVLMSIVGYLPVQAQIRLGILGGVHSANVLETNSLPGW
                     DTAVKKFERSRTGFQVGVMVEVPLGHHGLFFQPSVVYTTKGRDYTRNNDSAGALLTDT
                     IYNKSLLGLGYVEVPLNLTYKIPITANRRNKIFISAGPYFGFFFNGHVTTESLTASSN
                     EYYSVTKPVMVGSGSDTYKTLDIGVNGRVGFEMGNIQIAGYFSQGLTSFYNATYPGTF
                     HHQVVGLSLGIWLTSTGTPTTKKKRDSDKDGITDDVDDCPLQPGTAAWHGCPAPDTDR
                     DGVDDDHDSCRTIPGVARYNGCPIPDTDHDGIDDEHDSCRTVAGLARYNGCPAPEPKV
                     DTVPAPVIQKETTEKVNFIARNILFNSSSDQLTDGSFDALDQLAGILLAHPELLLRIE
                     GYTDNSGNAAKNLTLSRKRAAAVKKYLVGKGISSTRLSSEGFGQERPIADNHTDAGKA
                     ANRRVELKLSVPEMRSGVPAGKN"
     gene            4952..6661
                     /locus_tag="VGQ51_08430"
     CDS             4952..6661
                     /locus_tag="VGQ51_08430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEV9036632.1"
                     /translation="MNRNPSGWLLVILLACLPGIVPAQGTSAKQDLPKGWHLMDKEHD
                     GYYGISVNKAYEFAHLKKLKSKTVLVAVIDSGVDTTHEDLKGVLWTNPKEIPGNGIDD
                     DHNGYVDDIHGWNFIGGKDGTNVKDDSQEEARVYYAFKEKFDSTGLDTAALSPDDKDN
                     YRMWVKARKTIMGDGSDNGIDLTQLHRALTACLRSDSILQQAMGKVEYTGNELDTFQV
                     RTTESRSAKAVFLYLFRANKIMDWTNKAFLTDFTEEVDREEKKAEVKETAPRDYRHEI
                     VKDNENDFSDRYYGNNDIMAGDPMHGTHVSGIIAAIRGNGKGMDGICDNVRIMMIRCV
                     PNGDEHDKDIALGIRYAVDNGARVINMSFGKNLSPEKPWVDSAVKYAESKGVLLIHAA
                     GNDNADVDTVDNFPNEHFLHSTYTASNWITVGASSDPLAEPGFNSYTASFSNYGKDEV
                     DVFAPGTRIYSTVPGSKYENLQGTSMAAPVVTGVAALILEFYPNLSAQQVKYCIEKSA
                     VAPPGKVKRPGTEDNPVMVNLSDISKTGGIVNAYEALKIAASLRPEKKEVPARSTLHN
                     LNN"
     gene            6665..7174
                     /locus_tag="VGQ51_08435"
     CDS             6665..7174
                     /locus_tag="VGQ51_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV9036633.1"
                     /translation="MIPRNEVIPAEFYQGYIDMIRDNDFREAIRKNTKLFHRLLESVP
                     RKKIDHAYAEGKWTIRQMLQHIIDAERVFAYRALRLSRKDPTPLPGFDEKQWAVNDGG
                     ADRKWKDLLEEFKAVRASTEYLFDSMSDDQLRFAGTVNDRPLNAFTIGFIIPGHVAHH
                     MRILEERYL"
     gene            7178..>7956
                     /locus_tag="VGQ51_08440"
     CDS             7178..>7956
                     /locus_tag="VGQ51_08440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9036634.1"
                     /translation="MNRVLSLPVRRVNRIAVSAFFFLAGFCYSSWCSRIPNIQQQLHL
                     DSAALGGVLLGLPVGLMTSLPLAGWLVAKLGSRPVAVTAALCYAATLPLLDMAAAPWQ
                     LFIALMCFGMCGNLLNISINTQAIGTEALYGRTIMASYHGLWSLAGFAGGSIGTLFIS
                     LGWLPWQHFLVITSFAVVTVILLSGRLIPVDAGSGENKPIFARPDRSLINLGIIAFCS
                     MICEGCMFEWSGVYFQNVVHPPRALVAVGYTAFMATMASGRL"
BASE COUNT         1909 a         2221 c         2124 g         1702 t
ORIGIN      
        1 acgccgctgg tcctgatcag caatatctac atcatcgcat tctactatct gaacacctac
       61 ctgttcctgc cgcggctgtt cgatcacaag cggttcgtat cctataccgt cctgatcctg
      121 gccctgctgg tgttctacgg attcctgacg cgtatctacc acggtgtctt cggctaccct
      181 accctgcctc agaacacggt gttcacccgg gcgcagaatc caagggcccg acctcttatc
      241 aattccggca atctcgcgct ctttctgctg gtgttcgtat tcagcaccgg gatccgggtc
      301 atcaaccaat ggctccggtc ggaacaacgc aataaggaga tagccaatga aaagctgaag
      361 gccgagctct cctttctcaa agcccagatc aacccgcatt tcctcttcaa tacgctcaat
      421 aatatatatg cgctggcctc cgcacagtcg gaaaataccg cgcccgcagt aatgaaactg
      481 tccagcatca tgcgttatgt gctgaccgag gcgcgtaatg acctggtccc gctggagaaa
      541 gagatccagt tcacctcgca ttatatcgag ctgcaaaaaa tgcggctcac cgacaaggcc
      601 gtcgtcgatt tcaccgtccg cggcgaaccc ttcggccgcc agatcgcccc gctgctatta
      661 cttccctttg tcgagaacgc ctttaaatac gggatcagca cccgggaacg cagccccatc
      721 gccatcctgt tggagataaa aaaggaaacg ttcgaagtca gcatctgcaa ccataaatat
      781 ttcaatacga tgttgcgggc aagtgataat accggtatcg gcatcaataa cacccggcgg
      841 cggctcgatc tgttctatga agaccgctat aagctcgata tcgacgataa gcccaacgaa
      901 ttttccgttc atttaaaaat cccggtataa tgttaacgtg cattgccatc gatgacgagc
      961 cgctggctct ggaggtcctg aagaaatata tcgccaagat ctatttcctg gatttgaaag
     1021 gcgtcttcac cgatcctttt gaagctcaga aagtgatgga ctccgcaccg gtggacatcc
     1081 tcttcctcga tatccaaatg cccgatatca acggcatcga gttctcgcgc atcatcaaca
     1141 aaaagaatac cgcggtcgtc tttaccactg cattcagcga atatgccgtg gacggcttca
     1201 atgtcgatgc catcgactat ctgctcaagc ccatcgaata cgaccggttc ctcaaggccg
     1261 tttacaaggc gaaggaatat gtcgactacc tcaccagcca ggagctgcag gaaggttaca
     1321 tcttcgtaaa atcggattac cagatggtga aggtgaacct gaaagagatc ctctatatcg
     1381 aaggtctgga cgattatatc aagatctatc tccccggaaa atccatcctg acgctgatga
     1441 cgctgaagac catcgcccag aagctgccgg cgcgggaatt catccgcgca caccgcagct
     1501 atatcgtccc catcagcaag atcgaacata tcagcaaaag caagatcaag atcggcgata
     1561 aggagatacc catcggcgtg agttacagcg agagcttttt cgcggccatg gaaaataaat
     1621 tgaattgatt gcggcgccct tcgggcgccg ccaggcaacc acccggggta tcttttcgta
     1681 ttgtagttgc cggggtggta gatcccggca gaagaccagt tttttcatag tagattttaa
     1741 gttaaggtcg gggccactgg ttagtggtta gttttttcct aaattgcttg cgatctctaa
     1801 gatcgcaagc ttatttatgg aattccttca acaactgcat atcggccggg aaaatgccgg
     1861 cacctccacg ggaacccaat ggcttccctc cacggcctct gtcctcgagt ccttttcacc
     1921 ggtggacggc aaacgtatcg cgactgtcac cactaccgat aaagccggct atgaagccgt
     1981 cgtcagcgcc gctcaaaaag cctttgccga gtggcgggaa tggcccgcac ccagacgtgg
     2041 cgaggtggtc cgccagatcg gagaagccct ccgggaacat aagaatgacc tcggacgcct
     2101 ggtttcctac gagatgggca agagccttca ggaaggttat ggagaagtcc aggaaatgat
     2161 cgatatctgc gatctggccg tcggtctttc ccgtcagctc aacggtctga ccatgcatag
     2221 cgaacgtcct gcccatcgca tgtacgagca atggcatccg cttggcgtgg taggcattat
     2281 ctccgccttc aatttcccgg tggccgtatg gagctggaat gccatgatcg catgggtctg
     2341 cggcaatacc tgtgtctgga agccgtctga gaagacaccg ctctgtgcgc tggcatgcca
     2401 gcatatcgtt tcaaccgttt ttacagcgaa caaggtcccg gaaggagtct gcggactcgt
     2461 cacgggagcc cgcgaaccgg gcgaatggct ggcctccgat gccaggatcc cgctgatctc
     2521 cgctaccggg tcgacccgga tgggcagggc ggtaggcata gccgtcgcga cacgtcttgg
     2581 gagatctatc ctcgagctgg gtggtaacaa tgccatcgtc atatcctccg acgctgatct
     2641 gaatatcgcc atacccggag ccgttttcgg agcggtcggt acggccggtc aacggtgtac
     2701 gaccacccgc cgtctcatca tacaggacgc catctacgat aaggtaaagg agaggctgaa
     2761 gactgcctat ggacagctgc gcatcggcga tcccctcgat gagaataacc atgtcggacc
     2821 cctgatcgat aaagacgccg tcggtcttta tcggtctgcc atcgaccagg ccaaacagca
     2881 gggctgccgg ttcatcatcg atggtgaagt gctcaccggc gacggcttcg agagcggatg
     2941 ctatgttcgc ccctgtatcg cagaagcgaa gcccgacgac ccgatcgtgg cgcaggagac
     3001 cttcgcgccc atcctgtacc tgctgcgata tggcagtctc gacgaggcca tcgccatcca
     3061 gaatggcgta ccacagggtc tttcttcttc gatcatgacg ctcaatcttc gcgaagccga
     3121 acggttcctt tcggccgcgg gcagtgattg cggcatcgct aatgtcaaca tcggaacgtc
     3181 cggtgcggaa attggcggcg cattcggcgg ggaaaaagaa acgggaggag gaagagaaag
     3241 cgggagcgat gcctggaagg cctatatgcg gcgtcaaacc aatacgatca actactcgac
     3301 ccaattgcct ctcgctcaag gtataaaatt caatgtataa atccgggtcg cgggaaagac
     3361 ccgtgtgccg atgaagctat ttccggcaaa ataatgttgc agggaggggc gttattagcc
     3421 gatgagaaaa tgggcgccca tcgttttaat gtctattgtc ggctatctcc ccgtgcaagc
     3481 gcagatacgt ttgggtatcc tgggcggcgt acattctgct aatgtattgg aaaccaacag
     3541 ccttccgggc tgggacacgg ccgttaagaa attcgagcgc tcccgcacgg gttttcaggt
     3601 cggcgtcatg gtggaagtcc cgctcggaca tcatggtctt ttctttcagc cttccgtcgt
     3661 ctacaccacg aaaggtcgcg actacacccg caataacgat tcggcgggcg cgctcctgac
     3721 ggataccatc tacaataaga gtctcctggg attgggatat gtcgaagtgc cgctgaacct
     3781 gacgtataag atcccgatca ccgccaaccg gcgaaacaaa atcttcatca gcgccggtcc
     3841 ttatttcggc ttttttttca acggccatgt gacgaccgaa agtctcaccg ccagcagcaa
     3901 cgaatactat agcgtgacga aacccgtcat ggtagggagc ggttcggata cctataagac
     3961 gctggatatc ggcgtcaacg gccgggtggg attcgaaatg ggtaatatcc agatcgccgg
     4021 ctatttcagc cagggtctca cgagttttta caacgctacc tatcccggga cctttcacca
     4081 ccaggtggtg gggctgtccc tcggtatctg gctgacctcc acgggtacgc cgaccacaaa
     4141 aaagaaaagg gattccgata aagacgggat caccgacgat gtggacgatt gtccgctgca
     4201 gccgggtaca gcagcatggc atggctgccc ggctcccgat accgatcgtg acggggtcga
     4261 cgacgaccac gattcctgcc ggactattcc tggcgttgcc cgctacaacg gttgtccgat
     4321 ccccgatacc gatcatgacg gcatcgacga cgagcacgat tcctgccgca ccgttgccgg
     4381 gctggcccgc tataacggat gccccgcccc cgaaccgaaa gtcgacactg tcccggcccc
     4441 cgtcatccaa aaggagacga ccgagaaggt caatttcatc gcccgcaaca tcctgttcaa
     4501 ttcgtccagc gatcagctga ccgacggctc gtttgacgct ctcgaccagc tggccggaat
     4561 actgctcgcg caccccgaat tgcttttgcg tatcgaagga tataccgaca attcgggcaa
     4621 tgcggccaaa aatctgaccc tgtcccggaa gcgggcggcg gcggtaaaaa aatacctcgt
     4681 agggaaaggt atctcttcga cccgtctgtc gtccgaaggt ttcggtcagg agcgtcccat
     4741 cgccgacaac cataccgatg ccgggaaggc ggcgaacaga cgcgtggagc taaaactgag
     4801 tgtgccggaa atgcggtccg gagtgccggc tgggaaaaac taactttaac aacttaataa
     4861 ggccattcct gtaatttttg atgacgtttg gcgcggcttt tagttatttt acaggattga
     4921 tcactacaca cactaactaa aatcaacgaa aatgaatcgt aacccgagtg gatggttact
     4981 ggttatccta ttggcctgcc tgccgggtat agttccggca cagggaactt ccgccaaaca
     5041 agacctgccg aaaggctggc atctgatgga caaagaacac gatggatatt atgggatcag
     5101 cgtaaataag gcctatgaat tcgcacatct gaagaaattg aaaagcaaga cggtgctcgt
     5161 cgccgtcatc gattcgggtg tcgatacgac gcatgaggac ctgaaaggtg tactctggac
     5221 caatccgaaa gaaatacccg gcaacggcat cgacgacgac cacaacggct atgtagacga
     5281 catccatggc tggaacttca taggcggaaa ggacggcacc aacgtgaaag acgactcgca
     5341 ggaagaagcc cgggtctatt atgccttcaa ggaaaagttc gatagcacgg ggctcgatac
     5401 cgctgctctg agccctgacg acaaggacaa ctaccggatg tgggtcaagg cccgcaaaac
     5461 gatcatgggc gacgggtccg acaatgggat agatcttacc cagctgcacc gcgcgctcac
     5521 cgcctgtctg agaagcgaca gcatcctgca gcaggccatg gggaaagtag aatacaccgg
     5581 caacgagctg gatacgttcc aggtaaggac tacggaaagc cgcagcgcca aagccgtatt
     5641 tctttatctg ttcagagcca acaagatcat ggactggacc aataaagcct tcctgactga
     5701 tttcaccgaa gaggtcgacc gggaggagaa aaaagcggaa gtcaaggaaa cggcgcccag
     5761 ggattaccgc cacgagatcg tcaaggacaa tgagaacgat ttcagcgacc gctattacgg
     5821 caacaacgat atcatggccg gagatccgat gcatggcacg catgtctcgg ggatcatcgc
     5881 cgcgataagg ggaaatggca agggcatgga cggcatctgc gacaacgtac ggatcatgat
     5941 gatccggtgc gtacccaatg gcgacgaaca cgataaggat atcgcgctgg gcatcaggta
     6001 tgccgtcgat aacggcgcgc gcgtcatcaa tatgagcttc ggcaaaaacc tttcgccgga
     6061 aaaaccctgg gtagattcgg cggtcaaata cgccgaaagc aagggagtcc tgctgatcca
     6121 tgccgcaggc aatgacaatg cggatgtgga caccgtcgat aattttccca acgagcattt
     6181 tcttcattcc acctatacgg cttcgaattg gataacggtc ggggccagca gcgatccgct
     6241 ggcggaaccc ggtttcaata gttataccgc atctttctcc aattacggta aagacgaagt
     6301 agacgtgttc gctcccggca cccgcatcta ttcgacggtg cccggaagta agtacgagaa
     6361 tctccagggc accagtatgg ccgcgcccgt cgttacgggt gtcgcagccc tgatcctgga
     6421 attctatccc aatctcagtg cacaacaagt aaaatactgc atcgagaaat ctgccgttgc
     6481 accgccgggg aaggtcaaga ggcccggaac ggaagacaac cctgtgatgg tgaacctgtc
     6541 ggatatcagt aaaaccggag gcatcgtgaa tgcctatgaa gcgctgaaga tcgccgccag
     6601 cttacgtccc gagaaaaagg aggtgcccgc gcggtccaca ttgcacaacc tgaacaatta
     6661 acatatgatc ccccgtaacg aggtcattcc cgctgaattc tatcagggct atatcgacat
     6721 gatcagggac aacgattttc gcgaagcgat ccggaagaac acgaagcttt tccaccgcct
     6781 gctggaaagc gtcccgcgaa agaagatcga tcatgcctat gccgaaggca aatggacgat
     6841 ccgtcaaatg ctgcagcata tcatcgacgc ggaaagggtg tttgcctatc gtgcgcttcg
     6901 tctttcgcgt aaggacccca ccccgctgcc gggattcgat gaaaaacagt gggccgtgaa
     6961 tgacggcggc gccgaccgta aatggaaaga tctgctcgaa gaattcaagg ccgtccgcgc
     7021 atcgacggaa tatctcttcg actccatgtc cgacgatcag cttcgtttcg ccggcacggt
     7081 gaacgaccgg cccctgaacg ccttcacgat cggcttcatc attcccggtc atgtggccca
     7141 ccatatgcgg atcctcgaag agcgctatct ttagcgtatg aatcgtgtgc tatcgctgcc
     7201 ggtccgaagg gtgaaccgta tcgccgtttc ggcctttttt ttcctggcag ggttttgtta
     7261 ctcatcctgg tgctcacgca tacccaatat tcagcaacag ctgcatcttg acagtgccgc
     7321 attgggcggc gttcttctcg gtctgccggt aggactgatg acctccctgc cattggccgg
     7381 atggttggtc gcaaagctcg gcagccgtcc ggtagcggtc accgcggcac tttgctacgc
     7441 ggctacccta ccccttctcg acatggcggc cgcaccctgg cagctattca tcgcgctgat
     7501 gtgttttggc atgtgcggca atcttctcaa tatatcgatc aacacccagg ctatcggtac
     7561 ggaagcccta tatggacgaa cgatcatggc ctcctatcac ggactatgga gcctggccgg
     7621 attcgccggc gggagtatcg gcacgctgtt catcagcctg ggctggttac cctggcagca
     7681 tttcctggtg atcacctcat ttgcagtcgt gaccgtgatc ctcttatcgg gacggcttat
     7741 tcccgtggat gcaggatcgg gagagaataa gcccatattc gcacgtcccg accggtcgct
     7801 gatcaatctt gggatcatcg cgttctgctc gatgatctgt gaaggatgta tgttcgagtg
     7861 gagcggcgtt tattttcaaa atgtcgtgca tccgccaagg gccctggtgg ctgtgggtta
     7921 tacggccttc atggcgacga tggcttcggg acgcct
//
LOCUS       DASYSV010000276         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1332270, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000276 DASYSV010000000
VERSION     DASYSV010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332270"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VGQ51_08445"
     CDS             <1..236
                     /locus_tag="VGQ51_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV9036635.1"
                     /translation="AEELRLTPDNMYEFGLIDSIIPEPLGGAHWDYTEAAGYLKPKLI
                     ETLDELRKHTPQERIEKRIEKYGKMGFFNVVSA"
     gene            264..1157
                     /gene="dapA"
                     /locus_tag="VGQ51_08450"
     CDS             264..1157
                     /gene="dapA"
                     /locus_tag="VGQ51_08450"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV9036636.1"
                     /translation="MTLRQQLRGTGVALVTPFKSDLSVDYAALERVIDFVIDGGVEYL
                     VTLGTTGETPTLSGNEKKAIAELTYEKAEGRVPVVAGIGGNNTAELVKELESFPLDKA
                     VAVLSASPYYSKPSQEGLFQHYKVLAEASPKPLLLYNVPGRTGRNLSAATTIRLAREV
                     PNIAGIKEASGDMGQCMEILRDRPEDFLVVSGDDALAFPQIACGMDGVISVVANSHPT
                     SFTEMTRMALRGDLKGAKALNDKLIAGYELLFAENNPAGVKGAMYELGLIGNYLRLPV
                     VPLSGPLQARMKEYVKGDKGR"
     gene            1204..>2281
                     /locus_tag="VGQ51_08455"
     CDS             1204..>2281
                     /locus_tag="VGQ51_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036637.1"
                     /translation="MRRALWVVGILALLLIAGYFIAVARVRKKIETALHTLPPGYSIT
                     YTSLHLRLLNGAFSLDDVHVRGNGQDGSIHELKVEGIRYAALLHHRLQVRAIRLDGCS
                     VDIGEGSAKMVKPEVALPFTAMSVEKVEIGGLRITGSKQRVSVEGEVKLDSLALDSGK
                     WSVGGVYLLARRIKSVIPGSDEAVTMDGVMLDSKKGVLRMDTVRLRPTENKEEIGKRR
                     GYQVDVMDALSEGIVVEGLDVMRLMRKQLVADRISVRKNDIYVFRDRRLPLKAGEKPL
                     PVEGLESLPVTLRVGSVSLGSTFFEYEEYPKLGKETGKLKIYRLHGTLEPLINHPRAG
                     DPAFLTMTMHGSLMNSGTVTATTKL"
BASE COUNT          534 a          531 c          744 g          472 t
ORIGIN      
        1 ccgccgaaga gctgcggctg acgcccgata atatgtatga attcgggctt atcgacagca
       61 tcatccccga accgctgggg ggcgcgcact gggactatac cgaggccgcc gggtacctca
      121 agcccaagct catcgagacg ctcgacgaac ttcgaaagca cacgccccaa gagcgcatcg
      181 aaaagcggat cgagaagtat ggcaaaatgg gattctttaa tgtggtatct gcctgacggc
      241 agctaccggt aacaccgata aatatgacgc ttaggcaaca gctccgggga accggggtcg
      301 cactggtaac accttttaaa tccgacctca gcgtggatta tgccgcgttg gaacgggtca
      361 tcgatttcgt gatcgatggg ggtgtcgaat atctcgtaac attgggcact accggtgaaa
      421 caccgaccct gtccgggaac gagaagaagg cgatcgcgga gctgacctat gaaaaggcag
      481 agggccgggt gccggtcgtc gcgggcatcg gcggcaataa tacggccgag ctggtgaagg
      541 aactggaatc attcccgctg gacaaggccg tggccgtatt gagcgcaagc ccctattata
      601 gcaaaccttc ccaagaagga ttgttccaac attataaagt actggcggag gcctctccca
      661 aaccgctgct gctgtacaat gtacccggca ggactggccg taacctaagc gcggcgacta
      721 ccatccgtct cgcacgcgaa gtgccgaata tcgcgggcat caaagaggct agcggggata
      781 tgggacaatg tatggagatc ctgcgggaca gacccgaaga ttttctcgtg gtcagcggcg
      841 atgacgcgct ggcttttcca cagattgcgt gcggcatgga tggggtgatc agcgtggttg
      901 caaacagcca ccctacctcc ttcacggaaa tgacgcggat ggcgctaagg ggcgatctca
      961 agggcgccaa ggctctcaat gacaagctta tagccgggta tgagctgctt tttgccgaga
     1021 acaaccccgc gggggtaaag ggcgcgatgt atgagctggg gctgatcggg aactatctgc
     1081 gtctaccggt ggtgccgttg agcgggccat tacaggcgcg gatgaaggaa tatgtaaagg
     1141 gcgataaggg tcgctgagca aaccgcaggc aaccacgacc gcgtgtccgg cgttatcttc
     1201 tttatgagac gggcattgtg ggttgtcgga atattggctt tgttgctgat cgcgggatat
     1261 tttatcgctg tcgcgcgggt gcgcaaaaag atcgaaacgg cgttgcatac gctgcctccg
     1321 ggttattcca ttacctatac ttctttgcat ttgcgattgt tgaacggggc attttccctc
     1381 gacgatgtac atgttcgcgg gaacggccag gatggttcca tacacgaact gaaggtcgaa
     1441 ggtatccggt atgcggcgct gctgcatcac cggttgcagg tgcgggctat acgcctggac
     1501 gggtgttcgg tggatattgg tgaaggttcg gcaaaaatgg tgaagccgga ggtggcactg
     1561 ccctttaccg cgatgtcggt ggagaaggtg gagatcggag gattgcggat aacggggagt
     1621 aagcaacggg tttcggtaga aggagaagtg aaactggatt cattggcgtt ggatagcggg
     1681 aaatggagtg tgggaggggt ttaccttttg gcgcgtagga tcaagagcgt tattcccggt
     1741 agcgatgagg cagtaaccat ggacggagta atgctggata gtaagaaggg tgtgcttcgg
     1801 atggatacgg tgaggctccg gccaaccgaa aacaaggagg agatcgggaa aaggaggggc
     1861 taccaggtgg atgtgatgga cgccctcagc gagggcatcg tggtggaggg tttggatgtg
     1921 atgagactaa tgcggaaaca attggtggcc gacaggatat ccgtccgcaa gaacgatatc
     1981 tatgtattcc gcgatcggcg tctgcctttg aaggcgggcg agaagccctt gcctgtagag
     2041 ggtttggaat cgctgccggt cacgttgcgt gtgggatcgg tgtcgctggg gagcaccttt
     2101 ttcgaatacg aggaatatcc gaaactcggg aaggaaacgg gcaagctgaa gatataccgg
     2161 ctgcatggaa cgctggaacc tctgatcaat catccgcggg ccggtgatcc ggcctttctg
     2221 accatgacaa tgcatgggtc gctgatgaat tcgggaacag tgacggcgac gacgaaacta
     2281 c
//
LOCUS       DASYSV010000277         3995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_434048, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000277 DASYSV010000000
VERSION     DASYSV010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434048"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VGQ51_08460"
     CDS             complement(<1..151)
                     /locus_tag="VGQ51_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEV9036638.1"
                     /translation="MIKITFPDGAVREYASGTTAMEIARSISEGLAKKVLAANVNGQV
                     WDATRP"
     gene            235..1365
                     /locus_tag="VGQ51_08465"
     CDS             235..1365
                     /locus_tag="VGQ51_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036639.1"
                     /translation="MRRLFWIVWLTMPVLVRAQNITGVWQGHFRSSNISARSSGLDER
                     YKFEVQIAQDGKTFEGVTYSYLSSFFYGKATASGTINPHTQKVLLQEGKLLEVRNSFG
                     DVCIMTCFLQYTKSGDEEFMEGTYVSMDTRDSGNCGRGTVFLRKVPTSDFYTEPMVLR
                     REQEMAVDSAREAKKLAMLKPATRPPAKHPVIAPPTGRRTTPTNKPSTAKTQPRTIPK
                     NQPPEPPLRTAPMARINVPKESVRTGDSSTGIGRKFPTIVPPVLKERENDVVKTLVIH
                     THEVTLNIYDDGAIDHDTVSVYLDNRQVINHAMLTDRPLIVTLHLDESNDYHEIVMVA
                     ENEGEIPPNTSLMIVKAGDKEYEVRITTTEQKNAVIKFQYAK"
     gene            1373..2236
                     /locus_tag="VGQ51_08470"
     CDS             1373..2236
                     /locus_tag="VGQ51_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036640.1"
                     /translation="MKRLRLFILLSLSSFLMQATLCVQAEDLNGIWKGTLTQGPGGCY
                     PSYYLELQINFNADRVTGRAYDYYDRNHFVKMSFTGRYNAQTHRLVLIENQVLQTMIP
                     EDCLPCIKTYDLNYSRNGDSEELTGDWKGLYSEKRLTCPPGKIHLKKAIVSDFPVDID
                     QNDTLAGLQATLHLQARKKEVVKTVTVKVPQIKIELYDNAEIDHDTVTVLINGKLLLY
                     RQMLTDRPLTIHFNALPGTEYELVMYADNLGDIPPNTALMMVTAGDQKFEVFMSSTLQ
                     NSAAVKFVFRP"
     gene            complement(2265..2882)
                     /locus_tag="VGQ51_08475"
     CDS             complement(2265..2882)
                     /locus_tag="VGQ51_08475"
                     /inference="COORDINATES: protein motif:HMM:NF023255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HEV9036641.1"
                     /translation="MKKFILLVVVFLAAGFLAWKLLRDKPVQAAPPKDEALRISKNPE
                     PFNTAFDSLLSQYFSVRDALVNWDTLKADQAAYTLAAKAEGLPIRLIRADTGIILTAQ
                     SIAAELSGNAKGFSEKPGIESRRQVFNTLSDNLYNLVRAVRFDKEIIYNIRCPMAFKD
                     SIEGFWLSNNSHIINPYLGNKHPLYKNKMIGCGEVVDSFDLVGKN"
     gene            3009..>3995
                     /locus_tag="VGQ51_08480"
     CDS             3009..>3995
                     /locus_tag="VGQ51_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9036642.1"
                     /translation="MLAGLQNVTFEFGARVIVKNATWHIQPNERIGLIGYNGTGKSTL
                     LKLFAGEYTPSEGTVERSRSTTIGYLHQDLLSFDTNGSILQVAMTAFERVLQVEKEIE
                     ELGKQLEKDHENNDLLIRYTDALHEMDTLDGYNIHHRTEEILQGLGFANSDLQRPFRE
                     FSGGWRMRVLLAKMILQQPDLLLLDEPTNHMDLPSIEWLEKYLQHYQGSVIIVSHDKY
                     FLNRMVTKIVELYQKELHIYNGNYNFYETEKAMRVELQQRAYENQQEYIRQQERFIER
                     FRAKATKAAAAQSAMKRLEKIERIEDVEIERPNIKINFRIDKTPGRILASLKG"
BASE COUNT         1022 a         1136 c         1005 g          832 t
ORIGIN      
        1 tcggacgcgt ggcatcccaa acctgcccgt tcacattcgc tgccaatact tttttcgcca
       61 atccttcgct gatggaccgg gcgatctcca ttgcagtggt cccgctcgca tattctctga
      121 ctgctccatc cggaaacgtg atcttgatca taaggacgca aatttatgaa ttttaccaat
      181 ggtagaggat gcaaaggatt atttatctaa tttgctgaag aggaaatcat gtgtatgcgg
      241 cgactttttt ggatcgtttg gttaacgatg cccgtcctgg tgcgggcaca gaacatcacc
      301 ggcgtctggc agggacattt ccgttcgtcc aacatttctg cccgatcttc tggattggat
      361 gagcgttata aattcgaagt ccagatcgcc caggatggca agaccttcga aggagtcacc
      421 tattcctacc tttcttcttt cttttacggc aaagccactg catccggcac catcaacccg
      481 catacccaga aggtattgct gcaggaaggc aagctcctcg aggtccgcaa cagtttcggg
      541 gacgtctgta tcatgacctg cttcctccaa tatacaaaat ccggcgacga agagttcatg
      601 gaaggtacct atgtgagcat ggatacgcgc gactccggaa actgcggcag gggcacggtc
      661 tttcttcgaa aagtgcccac atccgatttc tataccgagc ccatggtgtt gcggcgggaa
      721 caggagatgg ccgtggactc cgccagggaa gcgaaaaagc tggccatgct gaagcccgct
      781 acccgcccgc ccgcgaagca cccggtcatt gctccaccca ccggacgaag gaccactccc
      841 actaataaac cgtctaccgc caaaacacag ccgcggacca tcccgaaaaa ccaacctccc
      901 gaaccgcctc tccgcacggc gcccatggcc aggatcaatg ttccgaagga atccgtacgg
      961 accggcgaca gcagcaccgg catcggcagg aaattcccga cgatcgtgcc ccccgtgtta
     1021 aaagaaaggg agaacgatgt cgtcaaaacg ctggtcatcc atacccatga ggtcactctg
     1081 aacatctatg acgatggcgc catcgatcac gatacggtat cggtctatct ggataacaga
     1141 caagtcatca accacgcgat gctgaccgac aggccgctga tcgtcaccct gcatctggac
     1201 gagtcgaacg attatcatga aatagtgatg gtagccgaga acgaaggcga aatacctccg
     1261 aacacgtccc tcatgatcgt aaaagccggc gataaggaat acgaggtcag gatcaccact
     1321 acagagcaaa agaacgcagt aataaaattt caatacgcca aatgaccctg gtatgaagcg
     1381 cctgcgcctt tttatcctgt tgtcgttatc gtccttcctt atgcaggcga ccctctgcgt
     1441 gcaggccgaa gacctcaacg gtatttggaa agggacgctg acccagggtc cgggcggatg
     1501 ctatcccagc tattacctgg aactgcagat caatttcaac gccgatcgtg tcacgggcag
     1561 ggcctatgac tattacgacc ggaaccattt tgttaagatg agcttcaccg gccgctataa
     1621 cgcgcaaacg caccggctgg tgctcatcga gaaccaggtc ctgcagacca tgatcccgga
     1681 ggattgtcta ccctgtatca agacttatga tctcaactat tcccgaaatg gcgactccga
     1741 agagctgacg ggagactgga aaggtcttta ttcggaaaaa agactcacct gcccccccgg
     1801 caagatccat ctgaaaaaag cgatcgtttc ggatttcccc gtcgatatcg atcagaacga
     1861 taccctggcc ggtctgcagg cgacgctgca tctccaggcc cgcaaaaaag aggtggtgaa
     1921 gaccgtaacg gtcaaggtcc cgcagataaa gatcgagctc tatgacaatg ccgagatcga
     1981 ccacgatacc gtgaccgtgc tcatcaatgg aaagctcctg ctctaccggc aaatgctgac
     2041 cgaccggcct ttgacgatcc atttcaatgc ccttccgggc accgaatacg aactggtgat
     2101 gtatgccgac aatctcggcg atatcccccc gaataccgcg ctgatgatgg tgaccgcggg
     2161 agatcagaaa ttcgaggtat tcatgtcctc tacgctgcag aatagcgcgg cggtgaaatt
     2221 cgttttcaga ccctgatgcc gccaccgcgt gtgcccgccg gggatcaatt tttcccgacc
     2281 aggtcgaacg agtccacgac ctccccgcag ccgatcatct tattcttgta aagcggatgc
     2341 ttattcccca ggtaaggatt gataatgtgg gaattgttac tcagccagaa accttcgatg
     2401 gagtccttga aggccatggg acaacgtata ttatagatga tctctttgtc gaaacggacc
     2461 gccctcacca gattgtacag gttgtcggac agggtattga agacctgccg gcggctttct
     2521 atgcccggct tttcagaaaa gcccttcgca ttcccgctca attccgcggc tatgctttgc
     2581 gccgtgagta tgatgccggt atccgccctg atcagccgga tcggcagacc ttcggctttt
     2641 gccgccagcg tatatgcggc ctgatcggcc ttcagagtat cccagttcac cagcgcgtcg
     2701 cggaccgaga aatattgcga taacagggag tcgaaagcgg tattgaacgg ctcggggttc
     2761 ttcgatatgc ggagggcctc atctttgggc ggggctgcct gcaccggctt gtcccgcaac
     2821 aacttccagg caagaaatcc tgccgcaagg aatacgacga ccaacaaaat gaatttcttc
     2881 atttaaacgg attttcgcga agataccatg tcgcctccgg attaccgcaa aaatgtaatt
     2941 ctccgttgac ggacgatcac cctctctccc gtttgggcaa atgccatcgg aagattaatt
     3001 ttgcacccat gttagcagga ctgcagaacg tcacattcga attcggggcc cgggtcatcg
     3061 taaagaacgc cacctggcat atccaaccca atgaacgcat cggcctcata ggctacaacg
     3121 ggacaggaaa atccactttg ctgaaactgt tcgccggcga atacacgcct tccgaaggaa
     3181 ccgtggagcg cagccgcagt acgaccatcg gctatctcca ccaggacctc ctcagcttcg
     3241 ataccaacgg atcgatcctg caggtggcca tgacggcctt cgaacgggtg ctgcaggtgg
     3301 aaaaggagat cgaagagctc ggcaaacagc tggaaaaaga tcacgagaat aacgatctgc
     3361 tgatccgcta taccgacgcc ctccacgaaa tggacacgct ggacggctat aacatccacc
     3421 acaggaccga agagatactc cagggtctcg gatttgccaa ctccgatctg caaagaccgt
     3481 tcagggaatt cagcggcggc tggcgcatgc gcgtactgct ggccaagatg atcctgcagc
     3541 aacccgatct gctgctgctg gacgagccta ccaaccatat ggacctgcct tcgatcgaat
     3601 ggctcgagaa atatctccag cattaccagg gctccgtcat catcgtgagc catgacaaat
     3661 atttcctgaa ccgtatggtg acgaagatcg tcgagctcta tcagaaggag ctgcacatct
     3721 ataatggcaa ctacaatttc tacgagaccg agaaggccat gcgcgtcgaa ctccagcaac
     3781 gtgcctacga gaaccaacag gaatatatcc ggcagcagga gcgcttcatt gaaagattcc
     3841 gtgcgaaagc caccaaggcg gcggcggctc aaagcgcgat gaaaagactc gaaaagatcg
     3901 aacgcatcga agacgtcgag atcgagcggc cgaatatcaa gatcaatttc cgcatcgaca
     3961 aaacgcccgg aaggatactc gcttcgctga aaggc
//
LOCUS       DASYSV010000278         8670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2356821, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000278 DASYSV010000000
VERSION     DASYSV010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2356821"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VGQ51_08485"
     CDS             <1..568
                     /locus_tag="VGQ51_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9036643.1"
                     /translation="GVHLLDYGLLGMKAGVPKTIAALGGDFAYPYLYEETPDTLTTLY
                     EFDGFNLVWDHAMGIDNGSYGRDHGIAYIGNNGTLVLSRGGWEVIEEKGSKTKVAKPF
                     VKVSDDGLANHMANFVGVVRSRKMEDLHCSIEAGSHVATVAQMGNISFRSGKRLEWDA
                     GAGRFKDEAVNAQYLMKEYHNGYALPKV"
     gene            694..1593
                     /locus_tag="VGQ51_08490"
     CDS             694..1593
                     /locus_tag="VGQ51_08490"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV9036644.1"
                     /translation="MTKKLDSLAKNPDSSGGKQKNQVAPSYYNENTRFTPRIYVLLTL
                     DDIKQQWTSPFRATPKNWLQIGAFAGLTTGAVFLLDKPVNKYTVEQIRTNKSVVSASL
                     YVTNFGGWYETYTLAALGAYGIIFKKDKEKTTTLLATQAYVTGALIETVFKYLTSRQR
                     PSYYDAETGQNSHAFHGPFYHFMKPDHYSYSSFPSGHTTVAFAAATVFAMEYKNIWFV
                     PIISYSAATAIGISRIVQNAHWISDVLVGAGLGFITGRQVVNNYHRYSKLQLEKAKKK
                     NSLSFSLDFYNGTMVPGIVYTFR"
     gene            complement(1641..3230)
                     /locus_tag="VGQ51_08495"
     CDS             complement(1641..3230)
                     /locus_tag="VGQ51_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HEV9036645.1"
                     /translation="MKKRYILIFLLVSAKALLAQNPATDTIVPSKGDIQGQLKAIRAR
                     KVAWFKDSAGIEPRKIQGLDYTRYSRYGDLLNDDPEYNKKSPLWRPAVQVVIENTLLN
                     IMDRTVMHLEFAKTDAHSWSRNLKAGFPWTNGWEWDQDRFGNNFLSHPMMGSFYYSAA
                     RTNGYNFWASVPIVFAGSYMWKIFGENGTPEREDLINTTVDGVLLGEVTYRLSSNILD
                     DRATGAERVFREILAGLVNPVREVNRLWDGSYKRRVNKEIYQKEPVNIALFLGAHSIN
                     NETNALLSGHTDEMVNIQLNYGNPFERRSIKPFDYFKFRAEMNFGVGRKIVDNITGYG
                     ALLGRTYQFGGLNMLVGGFQHYDYYDAKAFELSTIAFGPGVLSRLRLSKSSNLDLNLH
                     LAFVPFAGSSVGPVTDTSFYRDYRFANGWEAKVEAAVNISKYATLSLWYYYYFIHAFD
                     NTGKDESPENSLGNNYIDVLKPRIAVHVYKTLSVGLEQNIYWNNHRQPPYTPLRYTQT
                     EQRIFLMFNWQDQQRRGRYYL"
     gene            complement(3399..4163)
                     /locus_tag="VGQ51_08500"
     CDS             complement(3399..4163)
                     /locus_tag="VGQ51_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEV9036646.1"
                     /translation="MKHPLLALAVLLTLSSATPIHRAPRTSAHPWPEIISKPKVLVFC
                     KTNGYHHASITTGIAAIRQLGEANGFAVDTTADSTWFRKKILRKYAALIFLSPTGKVF
                     GPAEEQALQDYIHHGGGFVGIHAATDCEYNWQWYGDLVGGYFKSHPHQQEARFFVVDS
                     TNPAMKGFPNPWMHFDELYNFKYLNPGIHVLLKIDESSYSGGANGENHPFSWYHDFEG
                     GRSFYTALGHTDATWSDPLFLHHLLAGIQYAMGTHP"
     gene            complement(4160..4861)
                     /locus_tag="VGQ51_08505"
     CDS             complement(4160..4861)
                     /locus_tag="VGQ51_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEV9036647.1"
                     /translation="MRTLLLMLAATLPALLSAQTPNSLTMAEKKDGWTLLFNGTSTTG
                     WHTYGQPSAGNRWIVQDGAFTPNPSAPGHGDLLTDGVYGDFDLKLEWKISPNGNSGVL
                     FLVHEDTTKYKETYFTGPEMQVLDNNGNPDAKNHAHRAGDLYDLIPCSRETVKPVGEW
                     NQVEIYSRGGQLKLFLNGVNVVSTTMWDDHWRDMIAHSKFKRWPDFGTFHTGRIALQY
                     HDFPVWFRNIKIKKL"
     gene            complement(4869..5297)
                     /locus_tag="VGQ51_08510"
     CDS             complement(4869..5297)
                     /locus_tag="VGQ51_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV9036648.1"
                     /translation="MKFSKNLVVIVSFTLLFAACGSGGDKTAASSKDSATSTAPGSGA
                     ATSSNPADDKAIGLIAGSDCTTCHRLHQADGGATIGPAYDQVAAKYNPAPDSTVDRII
                     NKIISGGSGVWGTVPMTPHPTLTKDDAKTIVAYILTLKKQ"
     gene            complement(5347..6402)
                     /locus_tag="VGQ51_08515"
     CDS             complement(5347..6402)
                     /locus_tag="VGQ51_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV9036649.1"
                     /translation="MTTIKGPAVFLAQFMGDQPPFNSLKDICKWAAGLGFIGVQLPSW
                     DPRVIDLQKAAESKTYADEIKGIVNGAGLQITELSTHLQGQLVAVHPAYDAQFDGFAP
                     AAVHNNPKARTEWAVQQLKYAAKASRNLGLDAHATFSGALLWPTVYPWPQRPAGLVDT
                     GFKELAARWLPILNEFDKQGVDVCYEIHPGEDLHDGITYEMFLQHTGNHPRACLLYDP
                     SHFVLQCLDYLEYIDNYHERIRMFHVKDAEFNPTGKQGVYGGYQSWIDRAGRFRSLGD
                     GQVDFKSIFSKLAAYDFKGWAVLEWECCIKNSEDGAKEGAIFIRDHIIRVTEKAFDDF
                     AGTGNDEKFNRTVLGLK"
     gene            complement(6413..7570)
                     /locus_tag="VGQ51_08520"
     CDS             complement(6413..7570)
                     /locus_tag="VGQ51_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9036650.1"
                     /translation="MNRKLRMGMIGGGKNAFIGAVHRIAANMDGLIELVCGAFSSHPD
                     NARESGKALFLPDDRIYLSYEEMMQKESRLPADKRMDFVAIVTPNHLHFSPAMLALEH
                     GFHVVLDKPMTFSLDEALRLQKKVNETGLLFCLTHTYSGYPLVKQARRMVKDGVFGNI
                     RKVYVEYPQGWLSRLSEKDGSKQAEWRTDPKRSGKSGAMGDIGTHAAHLAEYISGLRI
                     KQLCADLNIMVKGRALDDDGNVLLRFDNGATGVLYASQVAAGEENALKIRVYGENGGL
                     EWAQQEPNTLLVKWLDQPTQVYRAGMPWLSSDAKFNTRTPAGHPEGYLEAFANLYRDF
                     ALTLSARLDGTTPPPEATDFPSVEEGVRGMAFIDNLIRSNDSKEKWTTFTI"
     gene            7748..>8670
                     /locus_tag="VGQ51_08525"
     CDS             7748..>8670
                     /locus_tag="VGQ51_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9036651.1"
                     /translation="MTSSTTIQANKLFLASRIALIFTAMTFALRASVVDAWGSELHLT
                     NEQIGWIFSPAFYGFTIAMIFGGPLCDVLGMKRLMGLAFIGHISGCVIYLLAGSATML
                     FVGTLCIGIGNGMVEAACNPLVVTLYPNSKIKMLNRFHVWFPGGITLGSLIFYLMMER
                     FHMGWHSLIFILFIPAIIYGIMMWGLEFPKTERVTTGVSTKAMFLSCLTPLFLVMLAC
                     MFLTGATELATDQWIKALLTGVGASGLLVLAFINVIMAVGRSFAGPVVHRLNPNGMLI
                     FSSIFACLGLILMSRASGYAAFGAAFVFAAGV"
BASE COUNT         1828 a         2017 c         2615 g         2210 t
ORIGIN      
        1 gggtgtccat ctgctggatt atggattgct gggcatgaag gcgggcgtcc ctaagaccat
       61 agccgcattg ggtggagatt ttgcatatcc ttatctgtat gaagagacgc cggatacgct
      121 gactaccctg tatgaattcg atggtttcaa cctggtttgg gatcatgcga tgggaatcga
      181 caacgggagt tatgggcgtg atcatgggat agcgtatatc ggcaacaatg ggacgctggt
      241 gctcagccgc gggggatggg aggtaatcga agagaagggt agtaagacaa aggtggctaa
      301 gccgtttgtg aaagtgtcgg atgacgggtt ggcaaatcat atggccaatt tcgtaggcgt
      361 ggtgcggtcg cgaaaaatgg aagaccttca ttgttcgatc gaggccggct cgcatgtggc
      421 tacggtggcg cagatgggca acatcagctt ccgcagcggg aagcggttgg agtgggacgc
      481 cggagcggga cgctttaagg atgaggcggt gaatgcgcag tatttgatga aggaatacca
      541 taatggatat gcgctgccga aggtttagag gttctccatg gtctttttgg cacgcaaaat
      601 gaaggagtac tatttatgta ctccccctgc agacttatag tattgttatt cttgctgata
      661 ccctttttgg catcgtccca gcaaaaggat actttgacca agaaactgga cagcctggct
      721 aaaaatccgg atagttccgg cgggaaacag aagaatcagg tagccccctc atattataat
      781 gagaatacgc ggtttacgcc gcgtatctat gtcttattga cgttggatga cattaaacag
      841 cagtggactt cccctttcag ggctacccca aaaaactggc ttcagatagg ggcgttcgcg
      901 ggtttgacta cgggggcggt attccttctt gacaaaccgg tcaacaagta cacggtcgag
      961 cagatccgaa cgaacaagtc ggtggtatcg gccagtcttt atgtcaccaa tttcgggggc
     1021 tggtatgaga cgtatactct ggcggcgctt ggggcatatg gcattatctt caagaaggac
     1081 aaggaaaaga cgaccacgct tctcgcaacg caggcctatg tcacgggggc gttgatcgag
     1141 acggtgttca aatacctgac cagccgacag cgtccgtcgt attatgatgc cgagacgggt
     1201 cagaacagcc atgcattcca cggtcctttt tatcacttta tgaagccgga ccactattcg
     1261 tattcatctt ttccttcggg tcatacgacc gtggcctttg ccgcggcaac ggtgtttgct
     1321 atggaataca agaacatctg gtttgtgccg atcatttctt actcggcggc tactgcgatc
     1381 gggatcagca ggatcgtcca gaacgcgcat tggatctccg atgtcctggt cggcgccggg
     1441 ctcggcttca tcaccgggag gcaggtcgtc aacaactatc accgctattc caagctgcag
     1501 ctcgaaaagg caaaaaagaa gaattcgctc agttttagcc tggactttta caatggtacg
     1561 atggtgccgg ggattgttta cactttccga taaggaggag ggagggcggc gggcgcctcg
     1621 gtcatggcgt tgggggtaag ctacagataa taacgacccc gccgttgttg atcctgccag
     1681 ttgaacatca agaatatccg ttgttccgtc tgcgtgtagc gcaagggagt ataggggggc
     1741 tgcctatggt tgttccaata gatgttttgt tccagcccaa cgctcaatgt tttgtataca
     1801 tggactgcga tgcgcggttt cagaacgtcg atataattat tgccaagaga attttcgggg
     1861 gattcatcct tgccggtatt atcgaatgcg tggatgaaat aatagtaata ccaaaggctt
     1921 agggtcgcat atttactgat attgacggcg gcttccactt tcgcttccca cccgttggca
     1981 aagcggtaat ccctgtaaaa ggaagtgtct gtaaccgggc cgacgctgct tcccgcaaag
     2041 ggtacaaatg ccaggtgaag attgagatcg agattggagg acttactcaa acgcagtctg
     2101 ctgagcacgc ccggcccaaa ggcgatggtc gacagttcga atgcctttgc atcataatag
     2161 tcgtagtgct gaaatccacc tacgagcatg ttcaacccgc cgaattggta agttcttccg
     2221 agcagggcgc cgtaccccgt gatattatcc actatttttc tccccacgcc gaaattcatc
     2281 tcggctctga atttgaagta atcgaaaggt tttatcgaac ggcgttcgaa cgggttgccg
     2341 taattgagct ggatattcac catctcgtcg gtgtgtccgg agagcagggc attcgtttcg
     2401 ttgttgatgg agtgggcgcc gaggaaaagg gcgatattta ccggctcttt ctgatagatc
     2461 tctttattga cgcgtctttt ataactcccg tcccataggc ggttaacttc ccttacagga
     2521 ttgacgagcc ctgccaggat ctcgcggaaa acccgttccg cccccgtggc ccgatcgtcg
     2581 aggatattgg agctcaggcg ataggttacc tctcccagga gcactccgtc cacagtcgtg
     2641 ttgatcagat cttcccgttc gggtgtcccg ttctctccga agatcttcca catatagctg
     2701 ccggcaaaaa cgatcggcac ggatgcccag aaattgtatc cgttggtgcg ggctgcacta
     2761 tagtaaaagc tgcccatcat cggatgcgat aagaaattgt tgccgaacct gtcctgatcc
     2821 cattcccatc cgttagtcca gggaaagccc gctttgaggt tgcggctcca ggaatgggca
     2881 tccgtttttg caaattcgag atgcatcacg gtccggtcca tgatattcag aagggtgttt
     2941 tcgatgacga cctgcacggc gggtctccac aacggactct ttttgttata ttccggatcg
     3001 tcgttcaaaa ggtctccgta cctgctgtat ctggtatagt cgaggccttg aatttttcgg
     3061 ggttcgatgc ctgccgaatc cttaaaccag gcaacctttc gtgccctgat cgccttcagt
     3121 tgtccctgga tatcgccttt tgacggaacg atggtgtcgg ttgccggatt ttgggccaaa
     3181 agagcctttg cagacactag cagaaaaata aggatgtatc gctttttcat tgtagacgga
     3241 tgggggcaat gataagcaaa acaaaagatg gaccacttag accggaggac cattacaagg
     3301 catgcatagc gccatcctac ggcaatggca gaccgaaata tcgaaaacca gactttcttc
     3361 aggttccgga caggaatgtt gaaggtgggt aaaattttct acggatgtgt acccattgcg
     3421 tattggatgc ccgccagcag gtgatgcagg aacaggggat cggaccaggt ggcatcggtg
     3481 tgtcccaggg cggtatagaa ggaacggcct ccttcgaaat cgtggtacca ggagaagggg
     3541 tggttctcac cgttggcccc gccggaatag gaggactcgt cgatcttcag cagcacgtgg
     3601 atgccggggt tgagatattt gaaattatac agttcatcga aatgcatcca gggattgggg
     3661 aaacctttca tggccggatt cgtggaatcc acaacgaaga accgggcttc ctgttggtgg
     3721 ggatgcgatt tgaaatagcc gccgacgaga tcgccgtacc actgccagtt atattcgcag
     3781 tcggtggcgg catggatgcc tacgaaaccg ccgccatggt ggatatagtc ctgcagggct
     3841 tgttcttcag cgggcccgaa gaccttgcct gttgggctga ggaaaatgag ggcggcgtat
     3901 tttctcagga tctttttacg gaaccaggtg gaatcggccg tagtgtcgac agcaaagccg
     3961 ttggcttcgc ctagctggcg gattgctgct atgcctgtgg tgatgctggc gtggtggtag
     4021 ccgttggtct tacagaagac gaggactttg ggtttcgaaa tgatctcggg ccagggatgg
     4081 gcggaggtgc ggggcgcgcg atggatcggc gtggcgctcg agagtgtcag cagaacggcg
     4141 agggcgagaa gcgggtgctt cataatttct ttattttaat atttctgaac cagacgggaa
     4201 aatcgtgata ctgcagggct attctgccgg tgtggaaggt gccgaaatcc ggccagcgtt
     4261 tgaacttgct gtgtgcgatc atatcgcgcc agtggtcatc ccacattgtg gtgctgacca
     4321 cattgacacc attgaggaat agtttcaact ggcctcccct ggaatagatc tccacctgat
     4381 tccactcgcc gacgggtttg acggtctccc gcgagcaggg gataaggtca tagagatcac
     4441 cggcgcgatg tgcgtggttt ttggcgtcgg ggttgccgtt attgtccagg acctgcatct
     4501 cgggtcccgt gaaataggtt tccttgtatt tggttgtatc ctcgtggacg aggaacagga
     4561 ctccgctatt gccattgggg gagatcttcc attccagctt cagatcgaag tcaccgtaga
     4621 cgccatccgt gagcagatcg ccatgtccgg gcgcggaggg attgggcgtg aaagcgccgt
     4681 cctgcacgat ccatcgatta ccggcagagg gttggccata ggtatgccag ccggtggtgg
     4741 aggtgccgtt gaaaagaagc gtccatccgt cctttttttc ggccattgtc agggagttag
     4801 gcgtttgtgc tgacagcagg gcaggcagcg tcgccgcgag catgaggagc agggttctca
     4861 tcggaaggtt attgtttttt taatgtcagg atgtaggcga ctattgtttt ggcatcgtct
     4921 ttcgtgagcg tcgggtgcgg ggtcatgggt actgtgcccc atacgccgct gccgccggag
     4981 atgatcttat tgatgatacg gtcgaccgtg ctgtcgggcg cgggattgta tttggccgct
     5041 acctggtcat aagccggacc gatggttgcg ccgccgtctg cctggtggag gcggtggcag
     5101 gtggtacaat cgctgccggc gatgaggcca atggctttgt catcggcggg gttactgctt
     5161 gtcgctgcac cggacccggg ggcagtggaa gttgcgctgt ctttggagct ggcggccgtc
     5221 ttgtcgccgc cgctgccgca tgcggcaaag aggagggtga aggagacgat gacgacgaga
     5281 tttttgctaa acttcatatt caaattttta gttggaatgg tgacgggcca agttaggtat
     5341 ttctggttat tttaagccga ggaccgtcct gttaaatttt tcatcgttgc cggtgcccgc
     5401 gaagtcgtcg aatgcttttt cggtgacgcg gatgatatga tccctgatga agatagcgcc
     5461 ttctttcgca ccgtcttcgg aattcttgat acagcattcc cactcgagca cggcccagcc
     5521 tttaaaatcg tatgcggcca gtttgctaaa aatggatttg aagtcgacct gtccgtcgcc
     5581 gagcgagcgg aaccgacccg cacgatcgat ccaggattga tagcccccgt aaacaccttg
     5641 tttgccggtg ggattgaatt ccgcgtcctt cacatggaac atccggatgc gttcgtggta
     5701 gttgtcgata tattcgagat agtccaggca ttgaaggacg aaatgcgagg gatcgtataa
     5761 caggcaggcg cggggatgat tgccggtatg ttgcaggaac atttcgtagg tgatgccgtc
     5821 gtggaggtct tcccccggat ggatctcgta gcagacgtcg acgccttgtt tatcgaattc
     5881 gttgagaatg gggagccagc gggcggcgag ttctttaaag ccggtgtcta ccagtcctgc
     5941 gggtcgctgg ggccagggat atactgtcgg ccatagcagg gcgccgctaa aagtagcatg
     6001 ggcgtcgagg ccaagattgc gggaggcctt tgcagcgtat ttgagttgtt gtaccgccca
     6061 ttcggtacgg gccttcggat tattgtggac ggcggcgggt gcgaagccgt cgaactgcgc
     6121 gtcgtatgcc gggtgtacgg ctacgagctg gccctggagg tgggtggaga gttcggtgat
     6181 ctgaagacct gcgccgttga cgatgccttt gatctcgtcc gcgtaagtct tgctttcggc
     6241 tgctttttgg agatcgatga cgcggggatc ccaggagggg agctgtacgc cgatgaagcc
     6301 gaggcctgca gcccatttgc agatgtcttt gagggaattg aagggaggtt gatcgcccat
     6361 gaattgggcg aggaagacgg cgggtccttt tatggttgtc ataatgctag tgttagatgg
     6421 tgaaggttgt ccacttttcc ttgctgtcat tgctgcggat gaggttgtcg atgaacgcca
     6481 taccacgaac gccctcttcg acggaaggaa agtctgtcgc ttcgggaggc ggggtcgtgc
     6541 cgtcgaggcg ggccgagagg gttagtgcaa aatcgcggta gagattagcg aaggcttcga
     6601 gatagccttc ggggtggccg gccggtgtgc gtgtattgaa tttagcgtcg gaggacagcc
     6661 agggcatgcc ggcgcggtat acctgggtcg gctgatcgag ccattttacg agcagggtat
     6721 tgggttcctg ttgtgcccat tcgaggccgc cgttctctcc gtatacgcgt atcttcaggg
     6781 cgttttcttc gccggctgcg acctgtgagg cgtacaagac accggtggca ccgttgtcaa
     6841 aacggagcag gacgttaccg tcgtcatcga gtgcacggcc cttgaccatg atgttcagat
     6901 cggcgcagag ctgtttgatc cggagaccgc tgatatattc ggcgagatga gcggcgtgtg
     6961 tgccgatatc tcccattgcg ccgctcttgc ccgacctttt gggatcggtg cgccattcgg
     7021 cctgtttgct gccgtctttt tcggataggc ggctgagcca tccctggggg tattccacat
     7081 atactttacg gatattgccg aagacgccgt cctttaccat gcgacgggcc tgtttgacga
     7141 ggggatagcc ggaataggta tgtgtgagac agaagaggag accggtttca ttcacctttt
     7201 tttgcaggcg cagtgcttcg tcgagcgaga aggtcatggg tttgtcgagg acgacatgaa
     7261 agccgtgttc gagtgccagc atggccggag agaaatgcag gtgattgggt gtgacgatgg
     7321 caacaaagtc catgcgtttg tccgcgggaa ggcggctttc tttctgcatc atttcttcgt
     7381 aggagagata gatgcggtca tcgggcagaa aaagtgcctt gcctgactcc ctggcgttat
     7441 cgggatggct gctgaaggca ccgcaaacga gttcgataag accgtccata ttggcagcga
     7501 tgcgatgaac ggcgccaatg aaggcgtttt ttccaccgcc tatcattccc attcgtaatt
     7561 ttctattcat gctgttaatt taagggccgg agacccgctg caattttagg atttcttgct
     7621 gaaattagag attattagtt atgtttacat tgcgtctaaa tgacatctaa agcgccacgg
     7681 cgcaggatga gcgggagcgc cgcgggcgtt ccggcgcgca ggacgcgaac aaaaaaatcg
     7741 acttgccatg acctcgagca cgaccattca agcgaacaag ctatttctcg ccagccgtat
     7801 tgcgcttatt ttcaccgcta tgactttcgc tcttcgggct tccgtggtag atgcatgggg
     7861 tagcgaatta catttgacaa atgagcagat cggctggatc ttcagccctg cattttatgg
     7921 atttacgatc gcgatgatct tcggtggtcc gttatgcgat gtgctgggca tgaagcggtt
     7981 gatgggactg gcttttatcg gacatatttc gggttgtgtg atctatctgc tggctggcag
     8041 cgcaaccatg ttattcgtcg gcacgctgtg cataggcatc gggaatggaa tggtagaagc
     8101 tgcgtgtaat ccattggttg tgaccttgta tcctaatagt aaaataaaga tgttgaaccg
     8161 ttttcacgtt tggttcccgg gcggtatcac gctcggaagc ctcattttct acctgatgat
     8221 ggagcggttc catatgggct ggcattcatt gatcttcatt ttgttcattc cggctatcat
     8281 ttatggaatt atgatgtggg ggttggaatt cccgaagacc gagcgggtga cgacgggtgt
     8341 cagtacgaaa gccatgttcc tctcctgtct gactcctttg ttcctggtca tgctggcgtg
     8401 tatgttcctg acgggtgcaa ccgagctggc cacggatcaa tggatcaagg cgttgcttac
     8461 cggtgtaggg gcttcgggat tgttggtatt ggcctttatc aatgtgatca tggcagtggg
     8521 aaggtctttc gccgggccgg tggtacaccg gctgaatcct aacggtatgc tgatcttctc
     8581 ttcgatattc gcctgtctcg gtctgatcct gatgagcagg gcaagcggat atgctgcgtt
     8641 tggggcggcg ttcgtgtttg cagcgggggt
//
LOCUS       DASYSV010000279         8416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1715172, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000279 DASYSV010000000
VERSION     DASYSV010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715172"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2945
                     /locus_tag="VGQ51_08530"
     CDS             <1..2945
                     /locus_tag="VGQ51_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9036652.1"
                     /translation="NVSEKLQMEKGKEEAIRTLSYAMTYAKMGSWKLDFISSELSLSK
                     EFKALLAMDDEDSDKIPMDNFLHLYVVPEDFNLVAEEFAKAIQHKENTGYETSFSCRI
                     ITKQGWMRYLSVKGKVVDEQMEFGIAQDITFQKEAENALLNSEQKFRLLAENSEDIIS
                     VHAIDGTIWYLSPSVTTVLGYEVDDIIGRSFEQYVHPEDRRKFLPADQMPAFADKGAE
                     NSIIVRYRILRRDGTYIWLESIIKPIIDEAELIKLICTSRNITDQMIAQEKLKKKDHL
                     LHAVAQATHSLLINTDLNQAINESIQVLGSTAMVSRVFLFQNHFDTGTGQWSTTETHE
                     WTEKPVDFRLHHEYMHDIPFSSINKIVEPLLERQPFVSYKSTEEDEQLRGILDRTGVL
                     SSVALPIFLKNGMFWGFVGFDEMKEEREWYEAEFAILRSYASSLAAAIERKQIEIELV
                     QAKEMAESASHAKSEFMANMSHELRTPMNGIIGFTDLVLTTELQKAQRDYLQNVKKSA
                     YGLLEIINDILDFSKIEAGKLLIDHVSFKLDELIEEAIDMLTLKAFEKKLEMLYRVDP
                     TMPSQFLGDPVRIRQIVVNLLGNAIKFTGEGEIFVSIKKNSEVYESSGKTYIRLSIEV
                     KDTGIGIRPEKQQKIFESFTQADTSTTRKYGGTGLGLTISRSLAELMGGSLTVESEAG
                     NGSVFTLHLVLEIANQQPEVMLPQKALLKKVLIVDDNKSNLHLMQEIFGYFHIEAELA
                     GSAEEAIAKMKSARQVKEPFNLVLTDHLMPGMDGIAMVKEMKRQFPGNNMPYILMLSS
                     LEKNIYQHEAAKAGINKFLSKPVKMHELYGALLSLFEMTLQQDGQHFQMPGNNIEKIT
                     EPTSIMVADDDPINMLLISEVLRRMGFEVIQVSNGVEALERLPHCDPVLIFMDVNMPE
                     MDGYSTTREIRKMPEPWCNLPIIALTADAMKGDREKCLEAGMNKYISKPFRLEEIDEV
                     LRSYLLVV"
     gene            complement(2927..3484)
                     /locus_tag="VGQ51_08535"
     CDS             complement(2927..3484)
                     /locus_tag="VGQ51_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036653.1"
                     /translation="MRSAILVMAPVILSASLTAQGVFSNKTQVILEKVIQDYPNHFLH
                     LRGELIGQATQPARYRSTLQLPGSASSTVVVTGAGIQGSGWSCTILETSDFNTARVRF
                     AEIYDQLSNSIISADGQKTYILSGQYEAPDQNRRNTKVLFSLLPGVGEMKGVRVELAL
                     DQEEGHWVLSINVNDKDLPVYTTSR"
     gene            3619..3786
                     /locus_tag="VGQ51_08540"
     CDS             3619..3786
                     /locus_tag="VGQ51_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036654.1"
                     /translation="MKKEQLKHAVRKTRIYKGIYRPSSNLSRFLTTLLSILLQGQSPA
                     GGSFTDRRRRG"
     gene            complement(3794..6661)
                     /locus_tag="VGQ51_08545"
     CDS             complement(3794..6661)
                     /locus_tag="VGQ51_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9036655.1"
                     /translation="MKGVLIDSSEGHQPMANATISVTPLAGDSSDAEYTVSDKKGAFS
                     MRGISRGQYRLLITYEGYQAIDKRFSISDSGAVIDFSTLYMQRADKMLAAVVIQRPPM
                     SVHHDSLEFNAGSFAVKPNAVAEDLLKKLPGVTVDNSGNITAQGEKVQRILVDGKRFF
                     SDDPKLATRNLPPDIIDKIQVFDDLSDQSKFTGFDDGNRVKTINIVTKKNSRKGYFGK
                     TVAGDGTNENYDLSINMHRFDGNSQLSVLGQGNDINKQNFTPQDIFGGGGGGGGRRGG
                     GGAQASTSPQSNGVTTVWAGGLNYRNTYGTNPNHSTDVYGSYFYNSQHVSVDQIDSSI
                     SPVANNKGVDSAFVTSGFNNNINRISNHRVSLNIESRLDTNNSLVFRPSISFQKSTPN
                     GSSYSLETDNHGNPINSSRSTTSSENSGFSIGGSNLQFRHRFSKPYRTLSLDINTVAS
                     INNGTGFNNSLNNIYIRNRIDTLNQFYFDSLKSWTVTPTLNYTEPIAKNQILQFSYNH
                     VFNRSTSINRTYDFENATGGYTRFDSLFSNSYRFRSDADQVSLAWRIQHGQKFNLSVG
                     SGVQWTSFTSVNTTKDTSVARNYVNITPTLNLSYQFSNTSHLRVNYSGRTGTPSAAQL
                     QPLTTTSDSISFQIGNPLLKPQFTHSLRILYANFDPGSQRVIFATVNASTIVNDIQSA
                     QYTNALGGRNSTYVNLGGTYNVSGFFNYGFPLKRPKSNLNFMTNITYAQSQTLLAQDS
                     ISFVNHQFLHQYTRNTGLNETIRWTTNIKKNFDMNFSSTTGYTINKNPGAFSNGSSAA
                     DSSKSGTQGVSTSNNLNYFTETLGAEFTAYTNNGWLVAANFDYTYTYTNSKTFNASIP
                     LFTPSIAKQLFKKKNGEIRLTVFDVLNKNTSVSKSVGAGGAVSYNRTNVLSRYAMLTF
                     TWNLGNFAGSNQRRMPGFFPGRGGFRGGGGGGFRGGGEFRK"
     gene            complement(6936..>8416)
                     /locus_tag="VGQ51_08550"
     CDS             complement(6936..>8416)
                     /locus_tag="VGQ51_08550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV9036656.1"
                     /translation="LIIRLEEKYEQLREFNRIRELAPPDHLAPILRPYQTHGFHWLNY
                     LQEVNWGGILADDMGLGKTIQALSFLQHFRDENRRLNALVVCPTTLMFNWENEIKKFT
                     PSLTYHIHHGGDRTRNKETLSEYNVIITTYGTLRSDIKLLLEMAFDYAILDESQAIKN
                     PSSKVTKAACLLQARHRLCMSGTPLQNNTFDIFAQMNFLNPGMLGSLEFFRQEFAVPI
                     DKFGEQDRKDHLRKLLYPFILRRTKEQVAKDLPEKTETILFCEMEDEQRKVYEAYRND
                     YRDKILGTIETQGIQRSQLTILQGLMKLRQICDSPAILNETDKYPNHSIKLDELAREI
                     TENIGDHKALIFSQFLGMLALIRERLKELDVKFEYFDGSTSAQERERAIQSFQNDDSC
                     RVFLISLKAGGVGLNLTAADYVYIVDPWWNPAVEQQAIDRTHRIGQTKNIFAYRMICK
                     DTIEDKILQLQEKKRVLAKDLIADDDGFVKTLTKADVEYLFS"
BASE COUNT         1977 a         2157 c         2322 g         1960 t
ORIGIN      
        1 cgaacgtctc ggaaaagctg cagatggaga aaggaaaaga agaggcgatc cgcacgctgt
       61 cctatgccat gacctacgcc aagatgggtt cctggaaact ggacttcatc agcagcgagc
      121 tgtcgctaag taaagagttc aaggctctgc tggccatgga tgacgaagat tccgacaaga
      181 tcccgatgga caatttcctc catctgtatg tcgtccccga agacttcaat ctcgtcgccg
      241 aagaattcgc caaggcgatc cagcataagg aaaatacggg ctatgagacc tccttctcct
      301 gccgaattat caccaagcag ggctggatgc ggtatctttc ggtcaagggc aaggtcgtgg
      361 acgaacagat ggagttcggt atcgcccagg atattacctt tcagaaagag gcagagaatg
      421 ccctgctcaa cagcgagcag aaattccgtc ttctcgcgga aaattcggaa gacatcatca
      481 gtgtacacgc catcgacggc accatctggt acctttcacc ctccgtcaca acggtactcg
      541 gctatgaggt cgacgacatc atcgggcgaa gtttcgaaca atacgtgcat cccgaggaca
      601 gacgcaaatt cctgcctgcc gaccagatgc ccgccttcgc agataagggg gccgagaaca
      661 gcatcatcgt ccgctatcgc atcctccgca gggatggcac ctatatctgg ctggagagca
      721 tcatcaaacc catcatcgat gaagccgagc tcatcaaact gatctgcacg tcgcgtaata
      781 tcacagacca gatgatcgcg caggaaaaac tgaaaaagaa agaccatctc ctccatgccg
      841 tagcacaggc gacccattcg ctgctcatta ataccgatct caaccaggcc atcaacgagt
      901 ccatccaggt gctcggcagc acggccatgg tcagccgcgt cttcctgttc cagaaccact
      961 tcgacaccgg gaccgggcaa tggtcgacca cggaaacgca tgaatggacc gaaaaacccg
     1021 tcgatttccg gctacatcat gaatacatgc acgatatccc gttctccagc atcaacaaga
     1081 tcgtagaacc gctgttggag cggcaaccct tcgtcagcta taagagcacg gaggaggacg
     1141 aacagctgcg ggggatcctc gacaggacgg gagtattgtc cagcgtggcg ctgcccatct
     1201 ttctgaagaa cgggatgttc tggggattcg tcggtttcga tgagatgaag gaagaacggg
     1261 aatggtatga ggccgaattc gccatcctgc ggtcatatgc ctcttcactg gcggctgcga
     1321 tcgagcgcaa acagatcgag atcgagctgg tccaggccaa ggaaatggcc gagtccgcca
     1381 gccatgccaa gagcgaattc atggccaata tgagtcatga gctgagaacg cccatgaacg
     1441 gcatcatcgg atttaccgat cttgtgctga ccaccgaact gcaaaaagcg cagcgggatt
     1501 atctccaaaa tgtcaagaaa tccgcctacg gtttgctcga gatcatcaac gatatccttg
     1561 acttctcgaa gatcgaagcc ggcaaactgc tgatcgacca tgtcagcttc aagctggacg
     1621 agctcatcga agaggcaatc gacatgctca ccctgaaggc cttcgagaag aaattggaaa
     1681 tgctttaccg ggtggacccg accatgccct cgcaattcct gggtgatccg gtgcgtatcc
     1741 ggcagatagt cgtcaacctg ctcggcaatg ccatcaaatt taccggcgaa ggagagatct
     1801 tcgtctcgat caagaagaac agcgaagtgt atgagtcttc gggcaagacc tatatccgcc
     1861 tgtctatcga ggtaaaggat acgggcatcg ggatccggcc ggaaaaacag caaaagatat
     1921 tcgagagctt cacccaggca gatacctcta ctactcgcaa atacggcgga acaggtctgg
     1981 gtctcaccat ttccaggagt cttgccgagt tgatgggagg aagtcttacc gtggagagcg
     2041 aggcaggcaa tggcagcgtc ttcaccctgc atctcgtcct ggagatcgcc aaccagcagc
     2101 cggaggtgat gttgcctcaa aaggcactgc tgaaaaaagt actgatcgtc gacgacaaca
     2161 aaagcaatct gcatctcatg caggagatct tcggttattt ccatatcgag gccgaactgg
     2221 ccggcagcgc cgaagaggct atcgctaaaa tgaaaagcgc ccgtcaggtc aaggagcctt
     2281 tcaatctcgt gctcaccgac cacctgatgc ccggcatgga cggcatcgct atggtaaaag
     2341 aaatgaaaag gcagttcccc ggaaacaata tgccgtatat cctcatgcta tcctcgcttg
     2401 aaaagaatat ctaccagcat gaggcagcca aggccgggat caacaaattc ctgtccaaac
     2461 cggtgaaaat gcatgagctg tacggggcgc ttctctccct gttcgaaatg accctgcagc
     2521 aggacggcca gcatttccag atgccgggaa ataatatcga gaagatcacg gagcctacca
     2581 gcatcatggt ggccgatgac gaccctatca atatgttgct gatctcagag gtcttgcggc
     2641 ggatgggttt cgaggtgata caggtcagta acggcgtcga ggcgctcgaa cggctgccgc
     2701 attgcgatcc cgtcctgatc ttcatggacg tcaatatgcc cgagatggac ggctacagca
     2761 cgacacgcga gatccggaaa atgcccgaac cgtggtgcaa tcttcccatc atcgccctca
     2821 ccgcggatgc gatgaaaggc gaccgggaga aatgtctgga agccggcatg aacaaatata
     2881 tctccaagcc attcagactc gaagagatcg atgaggtgct gcgaagctac ctgctggttg
     2941 tgtagacggg aaggtcctta tcatttacat tgatggagag tacccagtgc ccttcttcct
     3001 ggtccagcgc gagttcaacc ctcacacctt tcatttcccc gacacccggc agcagcgaga
     3061 atagtacctt ggtattcctg cggttttggt ccggcgcctc gtattgacca ctcaggatat
     3121 aggtcttttg gccgtcggcg gagataatgc tgttgctcag ctgatcgtag atctcggcaa
     3181 aacggaccct ggcggtattg aaatcggaag tttcgaggat cgtacaactc cagccggagc
     3241 cctggatacc ggcgcccgtt acgactacgg tgctggaagc ggagcctggc aactgcagtg
     3301 tcgatctata ccgtgccggt tgtgtagcct ggccgatcag ctcgcctctg agatgaagaa
     3361 aatggttggg gtagtcctgg ataaccttct ccagtatcac ctgggtctta ttcgaaaaga
     3421 ccccctgcgc ggttagggaa gctgagagaa tgaccggcgc cataacaagt attgcgcttc
     3481 gcatatgaaa ggatttccag aaagatacaa tcctttcggc cccggcgcaa tagtaaatgt
     3541 ccggtgctat tttaacaaat cctacgcaat aaacaggtga actggccgtt acttccctga
     3601 atcccaaaat cctaaaagat gaagaaggaa cagttgaagc atgccgttcg aaaaacccgc
     3661 atctacaagg gcatatatcg cccttcctcc aatctttcgc gtttcctgac tacgctgtta
     3721 tccattctat tgcaaggtca gtcgcccgcg gggggatcat ttacggatag aagacgacgc
     3781 ggataaacgc ccatcacttc cggaattcgc caccgccgcg gaagccgccg ccaccaccgc
     3841 cacggaaacc accacggccg gggaagaacc ctggcatcct tcgctggttg gaacccgcaa
     3901 aattgcccag attccaggta aaggtcagca tcgcataacg cgaaagcacg ttcgtgcggt
     3961 tataggatac cgcaccgccc gctcccacac tcttggagac cgaggtattc ttattgagca
     4021 catcgaacac cgtcagacgg atctctccat tcttcttttt gaacagttgt ttcgcaatgc
     4081 tgggggtgaa cagcgggatg ctggcattga aggttttgga attcgtataa gtatacgtgt
     4141 agtcgaaatt ggcggcgacg agccagccgt tgttggtata tgccgtgaat tccgctccca
     4201 gcgtttcggt aaaataattc aggttgttgc tggtgctgac tccctgcgtg cccgatttgc
     4261 tcgaatcggc agcagacgaa ccgttggaga acgcaccggg attcttgttg atcgtataac
     4321 ccgtcgtaga actgaaattc atgtcgaaat tcttcttgat gttcgttgtc catcggatgg
     4381 tttcattcag cccggtattc ctggtgtact gatggaggaa ctgatggttg acgaaggaga
     4441 tcgaatcctg cgccagcagg gtctgactct gggcataggt gatattcgtc atgaagttga
     4501 ggttggactt cgggcgtttg agcgggaagc cataattgaa gaatccgctc acattatagg
     4561 tgccccccag atttacatag gtgctgttcc ttccgcccag cgcattcgtg tattgagcag
     4621 actggatgtc gttgacgata gtgctggcgt tgacagtagc aaagatcaca cgctgcgagc
     4681 cggggtcgaa attggcatac aggatacgga gcgagtgcgt gaactgtggt ttcaacagcg
     4741 gattgccgat ctggaagctg atgctgtccg aagtagtggt cagcggttgc agctgggcag
     4801 cgctcggagt acccgttctg cccgaatagt tcacccgcag atgcgaggta ttggagaatt
     4861 gatatgacag attgagggta ggagttatat tgacgtagtt gcgggcgacg gaggtgtcct
     4921 tcgtcgtatt tacgctggtg aagctggtcc attgtacgcc cgaccctacc gagaggttga
     4981 atttctggcc gtgctggatg cgccaggcca gcgacacctg gtcggcgtcc gagcggaatc
     5041 tgtacgagtt gctgaagagg ctgtcgaaac gggtatatcc accggtcgca ttctcgaaat
     5101 cgtaggtgcg gttgatggaa gtgctccggt taaagacgtg gttatagctg aactggagga
     5161 tctggttctt tgcgattggc tccgtatagt tgagcgtagg cgtcacggtc caggacttta
     5221 gagagtcgaa atagaactgg ttgagcgtat cgatcctgtt gcggatgtag atgttattga
     5281 gcgaattgtt gaagcccgtt ccgttgttga tactagcgac ggtattgatg tcgagcgaaa
     5341 gggtcctgta cggtttggag aaccggtggc ggaattgcag attggagccg ccgatactga
     5401 agccggaatt ctcgctggac gttgtgctgc gactgctgtt tatcgggttc ccgtggttgt
     5461 ctgtctccag gctgtaggaa gagccgttgg gcgtgctttt ctgaaaactg atgcttgggc
     5521 ggaagaccag cgaattgttc gtatccaggc ggctttcgat attgagcgac acccgatggt
     5581 tcgagatccg gttgatgtta ttgttgaagc cgctggttac gaaggcggag tcgactcctt
     5641 tattgttcgc caccggtgag atcgagctat cgatctgatc caccgagaca tgctgggagt
     5701 tgtagaaata gctgccatag acatcggtcg aatggttcgg gttggtgcca taggtattgc
     5761 ggtagttgag gccgcccgcc caaacggtcg tcacgccatt tgattgcgga ctcgtactgg
     5821 cctgcgcacc accgccgccg cggcgtccgc cccctccccc gccgccaccg aagatgtcct
     5881 gcggagtaaa gttctgtttg ttgatatcat taccctgacc cagcaccgac agttgcgagt
     5941 tgccgtcaaa ccggtgcata ttgatgctga gatcgtagtt ctcattcgtc ccatcgcccg
     6001 ctacggtctt cccgaagtat cccttgcggc tgttcttctt ggtgacgata ttgatggtct
     6061 tgacccggtt gccatcatcg aaacccgtga atttgctctg atcgctgaga tcgtcgaaga
     6121 cctggatctt atcaatgata tcggggggca gattacgcgt tgccaacttg gggtcgtcgc
     6181 taaaaaatcg cttcccgtca accagtatcc gctggacttt ttcgccttgg gccgtaatgt
     6241 ttccgctgtt gtcgacggtc acaccgggaa gttttttcaa aaggtcttcc gccactgcat
     6301 ttggctttac cgcaaaacta ccggcgttga attccagtga atcgtgatgg accgacatgg
     6361 gcggacgctg gatgacgacg gccgccagca ttttatcggc acgttgcata tagagggtcg
     6421 agaaatcgat gaccgccccg ctatccgaaa tagaaaaacg tttgtcgatg gcctgatagc
     6481 cttcatacgt gatcagcaac ctgtactgac cccggctgat cccccgcatc gagaatgcac
     6541 cttttttgtc ggagacagta tattccgcgt cggacgaatc ccctgcaaga ggggtgacgg
     6601 aaatggtagc attggccatc ggctgatggc cttccgagct gtcaatgagg actcctttca
     6661 cagagcctcg tatattagac tgtgagtatg aacgagttgt gaacaaaacc acagttgcca
     6721 acaaaaggat cacgaatttt ttcatagccg ggctttgaat attaccattg caaattgcct
     6781 gctaaaatac tcgctatccg gttgccgtca tatccggcaa ggtaccagtg gcaaaatgag
     6841 atgccggtag ggtgaagctg ccccgggaat ttggggggtg atcggtcaat aaaggcgcct
     6901 gaccggcgag gccagggcgg ggtatggata agggtctacg aaaagagata ttccacgtcc
     6961 gccttggtaa gggttttcac gaagccgtca tcgtcagcga tcaggtcctt ggccagaacg
     7021 cgtttcttct cctgtaattg caggatcttg tcctcgatgg tgtctttaca gatcatccgg
     7081 tacgcgaaga tgttcttggt ctgtccgata cgatgggtcc ggtcgatggc ttgttgctcg
     7141 acggcagggt tccaccacgg gtccacgata tagacatagt cggctgcggt aaggttgaga
     7201 ccgacaccgc ctgctttcag cgagatgagg aatacccggc atgaatcgtc gttctggaaa
     7261 ctttgaatgg cccgttcacg ttcctgcgcc gaagtgctgc cgtcaaaata ttcgaatttg
     7321 acatccagct ctttgagcct ttcgcggatc agcgccagca tgccaaggaa ttgcgaaaag
     7381 atcagcgctt tgtgatcgcc gatattctcg gtgatctcgc gggcaagctc atccagcttg
     7441 atagaatgat tgggatattt atccgtctca ttgaggatgg ccggcgagtc gcagatctgc
     7501 cgcagcttca tcaggccctg gagaatggtc agctgtgaac gctggatgcc ctgggtctcg
     7561 atggtgccga gtatcttatc gcggtagtcg tttcgatagg cctcgtatac ctttctttgc
     7621 tcatcctcca tttcacagaa aaggatggtc tccgtttttt caggaagatc ctttgccacc
     7681 tgctccttcg tccgccgcag gatgaacggg tatagtaatt tgcgaagatg gtctttccgg
     7741 tcctgctctc cgaatttgtc gatcggaacg gcaaattcct ggcggaagaa ctcgagactt
     7801 ccgagcattc ccggattaag gaaattcatc tgcgcgaaaa tgtcgaaggt attgttctga
     7861 agaggcgtac cgctcatgca aagccggtgg cgcgcctgca gcagacaggc tgctttggtc
     7921 accttgctgc tcggattctt gatcgcctgg gactcgtcga gaatggcgta atcgaaggcc
     7981 atttcgagca ggagcttgat atcgctgcga agcgtcccat aggtggtgat gatgacatta
     8041 tattccgata gcgtctcctt gttacgcgtg cggtcaccgc cgtgatggat atgataggtc
     8101 agcgagggcg tgaacttctt tatttcgttc tcccagttga acatcagtgt ggtgggacag
     8161 accaccagcg cgttcagtct tctgttctca tccctgaaat gttgaaggaa ggagagggcc
     8221 tggatggtct taccgagacc catgtcgtct gcaagtatgc cgccccagtt cacctcctgc
     8281 agatagttca gccaatggaa gccatgggtt tgatagggcc gcagaatggg cgccaggtga
     8341 tccggcgggg caagctcgcg gatacggttg aactcgcgga gctgttcgta tttttcctcg
     8401 agccggatga tcagct
//
LOCUS       DASYSV010000280         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2228191, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000280 DASYSV010000000
VERSION     DASYSV010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2228191"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VGQ51_08555"
     CDS             complement(<1..1099)
                     /locus_tag="VGQ51_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HEV9036657.1"
                     /translation="MSRILLPVLFAFLFVSAAEAQLLTPEKAAFTRQDSLRGSITPER
                     AWWNLLKYDISVTPDYNTKSVHGENAITFVALQDGQILQIDLQEPMKLLSATWRKKAL
                     TLTREGNVFHVRMPKMVKAGSVETIVCRYEGTPRVAVRPPWGGGWIWTKDEQGRPWMT
                     VADEGLGLSCWLPCKDHLYDEADSGVVLHITAADSLVAIGNGRLHDKHPNANGTTTWT
                     WVNLDPINSYDIIPYIGKYVNWSSEFAGEKGKLDCNFWVLDYNLEKAKPQFKQVDTML
                     AAFEYWMGPYPFYEDGYKLVEAPHLGMEHQSNIAYGNHFGNGYLGRDLSGTGWGLKWD
                     FIIVHESGHEWFGNNITDKDAADMWVHEGFTN"
     gene            1163..>2146
                     /locus_tag="VGQ51_08560"
     CDS             1163..>2146
                     /locus_tag="VGQ51_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV9036658.1"
                     /translation="MRSIWTSGFCLLLLGGCGGRGDRTDKKVFRYNEVDGIATLDPAF
                     AKNRSIIWAVHQLYSTLVETDDNLNIVPCLARSWEVGADRRTWTFHLRTDVYFQDDQV
                     FPGGHGRRLVASDIVYSLNRLLDPATASSGAWIFHDRIDTSGEGAFVAVDDSTFRLRL
                     IRPFHPILGLLTMPYCSAVPHEVVQRYGKEFRAHPCGTGPFRFKSWEEEQTLVFLRND
                     HYFERDSAGHPLPYLDAVKISFNTEKAAEFLQFQQGELDFVNEIDASFKDEILNKRGQ
                     LKKDWVGKIILSVSPQLNTEYLGILMDTTSDLVRHSPLRLLAVRQAINYGFD"
BASE COUNT          452 a          536 c          627 g          531 t
ORIGIN      
        1 agttggtgaa gccttcgtgt acccacatgt ctgcggcatc cttgtcggtg atattgttac
       61 cgaaccattc gtgaccgctc tcatggacaa taataaagtc ccatttgaga ccccagccgg
      121 tgccggacag atcgcggccc agatagccgt tgccgaaatg gttgccatag gcgatgttgc
      181 tttggtgttc catacccaga tggggcgctt cgacgagttt gtagccgtcc tcatagaaag
      241 ggtaggggcc catccagtat tcgaaggcgg caagcatcgt gtcgacctgt ttgaactgcg
      301 gttttgcttt ttcaaggttg tagtccaata cccagaaatt gcaatcgagc ttgccttttt
      361 ctcccgcaaa ttcggaggac cagttgacat actttccgat ataggggatg atgtcgtagg
      421 aattgatggg gtccaggttt acccaggtcc atgtggttgt gccgtttgcg ttcggatgtt
      481 tatcgtgcag acgtccgttt ccgatggcga ccaggctgtc ggcagcggta atatgcagta
      541 ctactccgct gtccgcttca tcgtagagat ggtccttgca gggcagccag cagctgagac
      601 cgagaccttc gtcggcgacg gtcatccagg gacggccctg ttcgtcctta gtccagatcc
      661 accccccgcc ccagggcgga cgtacggcga cacgcggcgt gccttcataa cggcatacga
      721 tggtttcgac gctgccggct ttgaccatct tcggcatcct gacgtgaaag acgtttcctt
      781 cacgggtcag ggtgagcgct tttttacgcc aggtggcgga gaggagcttc ataggttcct
      841 ggagatcgat ctggagaatt tgtccgtcct gtagtgcgac aaaggtgatg gcattctcgc
      901 cgtggacgga cttggtatta taatcaggtg tgacagagat atcgtatttc aatagattcc
      961 accaggcccg ttccggggtg atgcttccgc ggagtgagtc ctggcgggta aaggcggctt
     1021 tctcgggggt gagcagctgg gcttcagcgg cggagacgaa aaggaaagcg aaaaggaccg
     1081 gtagcagaat gcgactcatt gataatcttt attttggggt tatctttatt ttagaatgaa
     1141 gatacttaaa catttatttt gggtgcggtc gatttggacg agcggttttt gtttgctgtt
     1201 gttgggcgga tgcgggggta gaggcgaccg aacggataaa aaggtgttcc gatataatga
     1261 agtcgacggg atcgccacac tggatccggc ttttgcgaag aaccggtcca ttatctgggc
     1321 ggtccaccag ctgtatagta cccttgtaga gaccgatgac aatctcaata tcgtcccctg
     1381 tctggcgcga agctgggagg taggagccga ccggcgtacc tggacttttc atttgcgtac
     1441 cgacgtatat ttccaggatg accaggtctt tccgggtggc catggccggc gtctggtcgc
     1501 ttccgatatc gtctatagcc tcaaccggct gctcgatccc gcgacggcga gcagcggcgc
     1561 atggattttc catgaccgga tcgacaccag cggagagggt gcgtttgtgg cggtggacga
     1621 cagcaccttc cggcttcggc tgatacgccc cttccatccc atactgggtc tgctgacgat
     1681 gccctattgt tcggcagtgc cgcatgaggt ggtgcaacgg tatggaaagg agttccgcgc
     1741 tcatccctgc gggacgggcc cgttccgctt caaatcctgg gaagaagagc agacgctggt
     1801 ctttttacgc aatgatcact atttcgagcg cgacagcgcc ggccacccgc tgccttatct
     1861 ggacgcggtc aagatcagct tcaatacgga aaaggctgcc gaattcctgc aatttcagca
     1921 gggagagctg gattttgtca acgagatcga cgcctccttc aaagatgaaa tattgaacaa
     1981 acggggccaa ttgaaaaaag attgggtagg gaagatcata ttgtcagtgt caccgcagct
     2041 gaataccgaa tacctcggca tcctgatgga tacaaccagt gaccttgtac gtcattcacc
     2101 gttgcggctg ctcgctgtcc ggcaagcgat caattacggt ttcgat
//
LOCUS       DASYSV010000281         4454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2098976, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000281 DASYSV010000000
VERSION     DASYSV010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098976"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /gene="fucP"
                     /locus_tag="VGQ51_08565"
     CDS             complement(<1..1144)
                     /gene="fucP"
                     /locus_tag="VGQ51_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HEV9036659.1"
                     /translation="MSKKKFLFPFILVCTLFFMWAFLHNINGILIPHLKKVCRLSDTQ
                     SSFIDFAVYLGYFVFALPAGLFLHRWGYKKGILCGLFLFAAGALLFLPAADARNYSLF
                     LVALFVAAAGATFLETVANPYAANMGSPENPEQQLNFAQSFNGLGAVVAPYIGTHFIL
                     SGIEHTKGELDAMQARGELTAYLQQEANSVKPVYLVIAGLLILVAIAFVFTKLPEIKE
                     TEGAHGTEFSLRVLRLPHVRWAVIAEFFYVGAQVGVTSFLVRFSRYTANIGEKEAGDM
                     LGLAMVGFMAGRFLGTFFMKYIQPAKLLSLYSVINIGLLVVVLSAHGRVAVYALMAIP
                     FFLSIMFPTIFALGIKDLGEETKLASSLLVMSIIGGAFTPVMMGIIS"
     gene            complement(1335..3545)
                     /locus_tag="VGQ51_08570"
     CDS             complement(1335..3545)
                     /locus_tag="VGQ51_08570"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEV9036660.1"
                     /translation="MRFTWMLCMILPAGMAAAQTPPQQQDVLKAMQSANTYFMNKWPD
                     PGKQIVTNIARPSNIWTRAVYYEGLMALYSIDPKKEFYDYAVDWGEKHQWMPRNGVTD
                     RNADDQCAGQTWIDLYNIDPQPARIANIKTCIDNMVHSDKVDDWHWVDAIQMGMPVFT
                     RLGVLYKDTAYFRKMYELYQHTKTGLGLYSTLDHLWWRDKDFIPPYKEPNGQDCYWSR
                     GNGWVLAALVRVLSLLPVNEPHRPEYLQNYLDMVNALVPLQRADGFWNVSLHDSTHFG
                     GKEVTGTALFTYGMAWGIREGYLDKKIFGPVVWKAWKGLVRDALHPDGMLGYVQGTGK
                     EPKDGQPVSYDKIPDFEDFGLGCFLLAGSEVYQIAEVTTANLDESKVPSYEEPSLTLT
                     TAAAWKKDQRPAVYGLFEKNVYGRMPAVKIPVTYTTTSVDEHALGGAATRKDFTIHFL
                     PNDTAANLHMALYLPKGARHVPVFVSYSFMPLNAVEASSQWPLKDILSHGYGVAVAWY
                     WDLEADRANGWQTGIRTRLAEALRIEPYEWSAIGAWAWGLSRMADFLSTQQQVDAHRL
                     IVIGHSRLGKTAVWAGASDPRFAMVVSNESGEGGAALAKRDYGETIAIINDKFPWWFV
                     PAYKRYGSNTAAMPFDQWMLLDLIAPRPLYVASAMGDQWSDPKGEFLSTHLATPVYHL
                     FKEKGIETDSMPALEHPVGDGYIRYHIRSGKHDITLYDWQQYMDFADKRLRPAK"
     gene            complement(3562..>4454)
                     /locus_tag="VGQ51_08575"
     CDS             complement(3562..>4454)
                     /locus_tag="VGQ51_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036661.1"
                     /translation="KGVRWQKMTDPAGREAPSSVGSYLIWQQPHFIYLAELCYRAQKD
                     PTAAKALLEKYSTLVFSTADFMASYARWDNGTRRYVLGPGLIPAQERFREDSTYNPCF
                     ELAYWRWALEVAQHWRSRLGQASDAGWQRVIDGLSQLPQRNGLYYPTESASDAYTNPR
                     YRGDHPAVLATYGMLPATKGLDTAVLHATFEWIEKNWDWPSTWGWDYPMIAMTATRLG
                     LPEQAIDALLMPVKKNTYLPDGHNYQDDRLRIYLPGNGGLLAAIALMCAGFDGCQTAD
                     PGIPKDGKWQIKWEGLAPMP"
BASE COUNT         1017 a         1319 c         1152 g          966 t
ORIGIN      
        1 ccgaaatgat ccccatcatc accggggtga atgccccccc gatgatcgac atcaccagca
       61 acgaggacgc cagtttcgtc tcctccccga ggtctttgat ccccagcgcg aagatggtcg
      121 ggaacatgat ggacaggaag aacgggatcg ccatcagcgc atataccgca actcgtccat
      181 gagccgacaa taccaccacc aacaaaccga tattgatcac cgaatacaac gaaagcaatt
      241 tcgccggctg gatatacttc atgaagaaag tgcccaggaa tcgccccgcc atgaaaccca
      301 ccattgccag acccagcata tcacccgcct ctttttctcc aatgttagcc gtataccgcg
      361 agaatcggac gagaaagctc gtcacgccca cctgcgcccc tacataaaag aactcggcga
      421 tcacggccca ccgtacatgc ggaagacgga ggacacgcaa cgaaaattcc gtcccatgcg
      481 ccccttcggt ctctttgatc tcgggcagct tcgtaaagac aaaggctatt gcgaccagga
      541 tcaacaaacc cgctattacc aggtataccg gcttcaccga gttggcttcc tgctgcaaat
      601 acgcggtcaa ctcccctctc gcctgcatcg catccagctc gcctttcgta tgctcgatcc
      661 ccgacaggat aaaatgagtg ccgatataag gagccaccac ggctccaagc ccattgaacg
      721 attgcgcgaa attcaactgc tgctccgggt tttccggcga acccatattc gcggcatacg
      781 gattggccac cgtctcgaga aaagtagccc cggccgccgc tacgaacagc gctaccagga
      841 ataacgaata gttcctcgca tccgcagccg gcaggaacaa cagcgccccc gcggcaaaaa
      901 ggaaaagacc gcaaagaatt ccctttttgt atccccaccg gtgcaggaaa agccccgccg
      961 gcagagcaaa tacgaaatat cccaggtaca ccgcaaagtc gataaaggag gactgagtat
     1021 cgctgagccg gcagaccttt ttcagatgag ggatcagtat accgttgata ttgtgcagga
     1081 aggcccacat aaagaacagc gtacagacca ggatgaatgg aaataaaaat ttcttttttg
     1141 acatttagca agcgtttagc ccggtcgccg gggagaacca tccatcgcgc cgggagatca
     1201 aagaaaagtc attttgccgg ggaaaaggtc ccggatgtta tccgagtatt aagttatttt
     1261 aacacgaatt cagcgaatct aacgaatttc gtgaatattc gccggccaga cgcttcgtga
     1321 ggccgcccgg gtcttcattt tgccggtctg agccgcttgt cggcaaaatc catgtattgt
     1381 tgccagtcat ataaggtaat atcatgcttc ccgctacgta tatggtaccg gatataacca
     1441 tcccctacgg gatgctccaa cgccggcatg ctgtcggttt cgatcccttt ctctttgaat
     1501 agatgataca ccggcgtcgc cagatgggtg ctgaggaatt ctcccttcgg gtcggaccat
     1561 tggtcgccca tggcgctggc gacatacagc ggccggggcg cgatcaggtc gagcagcatc
     1621 cattggtcga atggcatcgc agcggtattg cttccatagc gtttataggc cgggacgaac
     1681 caccacggga atttgtcatt gatgatggcg atggtttcgc catagtcgcg cttggccagt
     1741 gctgcgccgc cttcgccgga ttcattggaa acgaccatgg caaagcgggg atcggaagcg
     1801 cctgcccaaa cagccgtctt gcccagccgc gaatggccga tgacgatgag ccgatgtgca
     1861 tccacctgct gttgggtgct caggaaatcc gccatacggg agagccccca ggcccatgcg
     1921 ccgatcgcgc tccattcgta aggttcgatc cgcaaggcct ccgcaagcct ggtccgaata
     1981 cccgtttgcc atccattggc gcggtcggct tcaagatccc agtaccaggc gacggcgacg
     2041 ccatagccgt gggaaaggat atccttcagc ggccactgcg aggaggcctc aacagcgttc
     2101 agcggcatga agctgtagct gacgaagacg ggtacatgtc gtgctccttt cggcagatag
     2161 agggccatgt gcaggttggc ggcggtgtca ttcggcagaa aatgaatggt aaagtctttg
     2221 cgcgtcgcgg cgccgccgag cgcatgttcg tcgaccgaag tggtggtata agtaaccggg
     2281 atcttaacgg cgggcatccg gccatagaca tttttttcga aaagcccgta gacggcgggg
     2341 cgttgatcct ttttccaggc cgcagccgtc gtcagcgtca gcgatggctc ctcataggac
     2401 ggcactttcg attcgtcgag attagcggtg gtcacctcgg ctatctggta aacctcgctg
     2461 cctgccagaa ggaaacaacc gagaccaaag tcttcgaaat cggggatctt gtcgtagctg
     2521 acaggctgcc cgtcctttgg ttctttgccc gtaccctgaa catatcccag catgccgtcg
     2581 ggatgcaggg cgtcgcgaac gagacccttc caggccttcc agaccaccgg tccaaatatc
     2641 tttttgtcga gataaccttc ccggataccc catgccatcc cataggtgaa aagcgcagta
     2701 ccggtgacct ctttgccgcc gaaatgcgtg gagtcgtgaa gacttacatt ccagaaccca
     2761 tcggcgcgtt gcaacggcac gagggcattc accatgtcca aatagttctg caggtattcc
     2821 ggccgatgcg gctcgttcac cggcaacagc gacaacacac gcaccagcgc ggcgagtacc
     2881 caaccattgc cgcggctcca gtaacagtcc tgtccattgg gttctttata aggcggtata
     2941 aaatccttgt cccgccacca gagatgatcc agtgtactat acaatccgag ccccgtcttt
     3001 gtatgctgat acagctcata catctttcga aaataggccg tatccttata aagcacgccc
     3061 agccgcgtaa ataccggcat gcccatctga atggcgtcga cccaatgcca gtcatccacc
     3121 ttatcgctat gcaccatatt gtcgatacag gtcttgatgt tggctatccg tgcgggctgt
     3181 gggtcgatgt tatacaggtc tatccatgtc tgaccggcgc attgatcgtc ggcattgcga
     3241 tcggtcactc cattccgcgg catccattgg tgcttctcgc cccaatccac ggcgtagtcg
     3301 taaaattcct ttttcggatc gatggaatac aaggccatta gtccctcata atataccgct
     3361 cgggtccaga tattgctcgg acgcgcgata ttcgtgacga tctgcttacc cgggtccggc
     3421 catttgttca tgaaataagt gttcgccgac tgcatcgctt tcaaaacgtc ctgctgctga
     3481 ggcggcgttt gggctgctgc cattccggcg ggcagtatca tgcacaacat ccatgtaaaa
     3541 cgcatatcca tcgattttaa gttaaggcat cggggccagt ccttcccatt ttatctgcca
     3601 ttttccatct ttgggtatcc ccggatccgc cgtttgacat ccatcgaatc cggcacacat
     3661 caatgcgatg gcggccagca atcccccatt acccggcaga tagatccgaa gccggtcgtc
     3721 ctggtaatta tgtccatcgg gaagataggt atttttctta accggcatca acaacgcgtc
     3781 gatcgcctgc tcaggcagcc ccaatcgcgt agccgtcatg gcgatcatgg gatagtccca
     3841 accccaggtg gatggccagt cccagttctt ttctatccac tcgaaggtag cgtgcaggac
     3901 ggccgtatcc aggccttttg tggccggcag catcccgtag gtggccagta ccgccgggtg
     3961 atcgccacgg taacgcggat tggtataggc atcggacgcg ctttcggtgg gatagtagag
     4021 accatttctc tgcggcagct gcgacaatcc atcgatcacc cgctgccagc cggcatcgga
     4081 cgcctgaccc aatcttgagc gccaatgttg cgcgacttcc agtgcccatc gccagtatgc
     4141 tagctcgaaa caagggttat atgtgctgtc ttcccggaac ctttcctgcg cggggatcag
     4201 ccccggtcca agaacgtatc tccgcgtgcc gttgtcccat cgcgcgtatg acgccatgaa
     4261 gtccgccgtt gagaacacca gcgtggaata cttctccaac aatgcttttg ccgccgtcgg
     4321 gtccttctgc gcgcgatagc acagctccgc caggtagatg aagtgcggct gctgccagat
     4381 gagataagag cccaccgaag agggagcctc acgacccgcc gggtccgtca tcttttgcca
     4441 gcggacaccc ttat
//
LOCUS       DASYSV010000282         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1715536, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000282 DASYSV010000000
VERSION     DASYSV010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715536"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2368)
                     /locus_tag="VGQ51_08580"
     CDS             complement(<1..2368)
                     /locus_tag="VGQ51_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9036662.1"
                     /translation="MKRISLLVFLTLSILSLHAQVVLQGRVVDDSTGTGLEATSVVLS
                     GTTKGTTTNSDGLFRLTIPSDGKKHSITISHAGYETTKQEVSGTEAGIVIRLKRANES
                     LNDVVVIGYQTVRRKDVLASVSSISSRDVRDIPLNSAPEALAGRLAGVQVTGSEGSPD
                     AQILIRVRGGGSITQSNNPLYVVDGVQIDNALNFIQLSDIASIDVLKDAAATAIYGAR
                     GSNGVVIITTKGGHNTGGKTTISYSGFAGVGTLSKELPVQDPYDFMYYQYERAQQTGD
                     TTGISPYGGYSWGTVQNYKNVPFYDWQKKMIGRNAFQQTHIVNLSGGSDKTQYYLSLA
                     HNDQDGVMLLSDYHRNTVNFRFDHQAATNLKIGANVRYNNTIVNGAGTSNPGSSGLNF
                     LRQIMRYRPFISEGQTPTSLDEAYANGTNANGLSLVNPVLLNQEQYRKSNQNILDLSA
                     YAEYRFTPWLSFRTTFGYDNNSQRKDAFDDTLTSNSKSNGAGMPIASIASTSLQTIDN
                     SNVFTYTNVNGHGKFHEHNTLTVLAGEETYQTDQSDVTVQTDYFPIGTTSGAALANMN
                     LGTPPSGTNLQEPKPVSTVIPVRQFSLFGRASYAFDDKYLAAVSFRADASSLFNPNIL
                     GRQWGYFPAVSAAWRISKEKFMSNVDWVTDLKLRAAYGQAGNNRISPYLYQTNFVTSV
                     NDGLGGQLITAFTPNNFSTPPLQWESTTSRNLGLDASFFNSRLGVSVDVYRNTTDNLL
                     INVPVSSIFGYTSRLQNVGATSNNGVEVQLNGMIMQRQTFTWSANFNIS"
     gene            2641..>3041
                     /locus_tag="VGQ51_08585"
     CDS             2641..>3041
                     /locus_tag="VGQ51_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEV9036663.1"
                     /translation="MSFHMWISERFFAGTSGLVLPVPNKQALPEKFRNKPRLTYYATL
                     FNTIEVNSSFYKVPKPSTFAKWVYEVPDEFRFTIKLWRGITHAPALHFRIKDVERFME
                     AVNNLSTTKGCLLVQLPPSTRADKSAQLQRLL"
BASE COUNT          635 a          804 c          868 g          734 t
ORIGIN      
        1 atgaaatgtt gaagttggcc gaccacgtga aggtctggcg ctgcatgatc atcccgttca
       61 gctgaacttc cacgccattg ttgctggtgg cgcccacatt ctgaagacgg ctggtatagc
      121 cgaagatgga agagaccggc acgttgatca gcaggttgtc ggtcgtattc cggtatacat
      181 ccaccgaaac gcccagccgg ctgttgaaga agctcgcatc caacccgaga ttccgggagg
      241 tggtggactc ccattggagg ggcggagtgg agaaattgtt cggagtaaag gcagtgatca
      301 gttgtccgcc cagaccgtca ttgacgctcg tcacgaaatt ggtctgatag aggtatgggc
      361 tgatccggtt gttaccggcc tgtccgtacg ccgcccgcag cttcagatcg gtgacccagt
      421 ccacattcga catgaatttc tccttcgaga tgcgccatgc cgccgataca gccgggaagt
      481 acccccattg tcttccgagg atgttcgggt tgaacagcga ggatgcatcc gcccggaagg
      541 acacggcagc caggtatttg tcgtcaaacg catagcttgc cctgccgaac agggagaact
      601 gccggaccgg gatcacggta gaaacgggtt tgggctcctg cagattggtt cccgaggggg
      661 gtgtacccag gttcatattc gcgagtgccg caccagacgt ggtcccgatc gggaaatagt
      721 ctgtttgtac ggtgacgtcg ctctgatccg tctggtaagt ctcttcccct gcaagcacgg
      781 tcagcgtatt gtgttcatgg aatttaccgt gtccgttgac attcgtataa gtgaagacgt
      841 tggagttgtc gatggtttgc agactcgtgg acgcgatcga ggcgatgggc attccggcgc
      901 cattcgactt ggaattggag gtcagcgtgt catcgaaagc atctttcctt tgactattgt
      961 tatcatatcc gaaggtggtc cggaagctga gccagggagt gaaccgatat tcggcataag
     1021 cgctcagatc gagtatattc tgattgctct tccggtattg ctcctggttg aggagcacgg
     1081 gattgacgag cgaaagaccg tttgcattgg tgccattggc ataggcctca tcgagggaag
     1141 taggtgtttg cccctcggaa ataaaaggtc ggtaacgcat gatctgccgc aggaagttaa
     1201 gcccggaaga acccgggttg gaggtacccg cgccattgac gatcgtatta ttgtaacgta
     1261 cgttcgcccc gatcttcagg tttgtggcgg cctggtggtc aaaccggaaa ttgaccgtat
     1321 tccgatgata atcggatagc agcatgaccc cgtcctggtc gttatgcgca aggctcagat
     1381 aatattgcgt cttgtccgag cccccgctca ggttgacgat atgcgtctgc tggaaggcgt
     1441 tgcgtccgat catctttttt tgccagtcgt aaaaaggtac gttcttatag ttctgcacgg
     1501 tcccccagct atagccccca tagggtgaga taccggtcgt atcccccgtc tgctgtgccc
     1561 gctcatactg atagtacatg aaatcgtacg gatcctgtac gggcagctct ttggataagg
     1621 tgcccacacc cgcgaaccca ctatagctga tggtggtttt tccgccggtg ttgtggccac
     1681 ccttggtggt gatgatcacc accccattgg aaccccgcgc gccatagatg gccgtcgccg
     1741 cggcatcttt cagcacgtcg atcgaggcga tgtccgacaa ctgtataaag tttagggcat
     1801 tatcgatctg gacgccgtcg acgacataga gcgggttgtt gctttgggtg atggacccgc
     1861 cgcctctaac tcggatcaat atctgcgcgt cgggtgagcc ttcggaaccg gtgacctgta
     1921 cgcccgcgag acggcccgcc agggcttcgg gtgcggagtt cagcgggata tcccttacat
     1981 ctcggctgct gatggacgat acggaagcaa ggacgtcctt acggcgaacc gtctgatagc
     2041 caatgacgac aacgtcgttc agtgattcat tcgccctttt cagccggatg acgatgccgg
     2101 cttcggtgcc ggatacttcc tgtttggtcg tctcatatcc ggcatgcgag atagtgatgg
     2161 aatgtttttt accgtcgctg ggaatggtca gacgaaaaag tccgtcggaa ttggtagtag
     2221 ttcctttagt cgtgcccgat aggacgacgg aagtcgcttc gagccccgtt cctgtgctat
     2281 cgtcgaccac ccggccctgt aggacgacct gcgcgtgcag gctgaggatc gaaagcgtca
     2341 ggaaaacgag gagtgagatt ctcttcatat tggcagttta gtttatacgt atgcaatctg
     2401 ccccaatctt agcgagaagt ttttgaaaaa ccacaggtta tcaagggttt atttacgcaa
     2461 tcgttcccgg gatcgtatgc ggctacgggt caaataaaag tacctccgcc gcactcgatc
     2521 ctccgtttgt cgggccttcg gccggcgcgc ccgtggcccg ccccggagtg cgatccaacc
     2581 cggcgttcgg cgcgttttcg gggtgcggtg atcgtgcaaa taaattggcc gcacctttgc
     2641 atgtctttcc acatgtggat ttcagaacgc tttttcgccg gcaccagtgg cctggtcctg
     2701 cccgtaccga acaagcaggc ccttcccgag aagtttcgca ataagccgag acttacttat
     2761 tacgcgacgc tcttcaatac cattgaagtc aacagcagct tttataaggt cccgaagccg
     2821 tcgacctttg ccaaatgggt ttatgaggtc ccggacgagt tccggttcac catcaaactg
     2881 tggcgcggta ttacccacgc accggcattg catttccgga taaaggacgt ggaacgattc
     2941 atggaggccg taaacaatct ctctacgacg aaaggctgtc ttcttgtaca attgccgccg
     3001 tctacacgtg ccgacaagag cgcgcagctc cagcgtctgc t
//
LOCUS       DASYSV010000283         3355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_434771, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000283 DASYSV010000000
VERSION     DASYSV010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434771"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..746
                     /locus_tag="VGQ51_08590"
     CDS             87..746
                     /locus_tag="VGQ51_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9036664.1"
                     /translation="MGAATKKIQVGIVDDHSLLRKALAKLVGSFESYAVLFEGDNGKE
                     IKNKITQHVIPDIIILDVNMPDMDGYETVRWLHKNYPQVKVLALSMFSDENTIIRMLR
                     LGAKGYILKNIEPEELKLALDSIMKKDFYLSEYISGKIISGLHKDLGNPEDPIVLNDK
                     EKEFLRLVCSEMSYKDIAEKMFISPRTVDNYRNTLFEKLKVRTRVGLVMFAIKNGLVE
                     I"
     gene            complement(721..3054)
                     /locus_tag="VGQ51_08595"
     CDS             complement(721..3054)
                     /locus_tag="VGQ51_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV9036665.1"
                     /translation="MWESIARLVLKYRLLWLFILLAVTGFMAYHASRVQLSYDFNSAI
                     PTDNPKYKAYQEFRKQFGEDGNLLFIGVQTDKLFQLPVFTQYIALDHALEKVSGVDDV
                     LGVATAVNLVRDTTLQKPKTVPIFPSNPVSQTQIDSGKAVLLGLPFYEGLLYNSKTNT
                     WLTAVHVNKAVMNSARRLETVGAIISLVDSFAVRTGLDMHYSGLPLIRTKMAKKVSTE
                     TTWFLLGSVCILSLILLLFFRSAGAMLLSLAVVIIGVIFSLGTMDLLGYKITLLNALT
                     PTLVVVIGIPNCIYFMNKYHTAYRDTGDKHEALIVMIGRMGIVTLFCNLTAAIGFGVF
                     ALTRSTILKEFGVVAGINILLLFVISFILIPVVLSFLPAPRAGHMRYLQNRYLSAILK
                     RLEIWTLHHQRPIYLLTVLIIGISVAGMFRLRSEGFIVDDLPQKDRLYTDLKFFETNF
                     KGVMPLEIVIDTKRKKGLRANLLKTIDSVEALSKYISAQPEMARPLSLVEGVKFLRQS
                     YYGGDSNSYDVPNPADLPFLSSYISSSGDTAARQARKNQLSQLVKSFVDTNQQKLRIS
                     INMADIGSQRLPLVLDDLKKKTAELFDSSKYRVEFTGTTVTYLEGSSFIIKGLKDSIE
                     WAFVLIAVCMLVLFRNFRILICSLVPNVIPLIITAGIMGWAGVRLRPSTVIVFSIALG
                     IAIDITIRFLVNYKQEKGASAQDAVIDTINTTGISILYTSSVLTAGFVIFCFSDFGGI
                     LALGWLTSLTLVIATITNLVFLPVLLLGLTRSRQGRS"
     gene            3118..>3355
                     /locus_tag="VGQ51_08600"
     CDS             3118..>3355
                     /locus_tag="VGQ51_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HEV9036666.1"
                     /translation="MELHKTKGIVLRTVKYGETSLIVTIFTELFGAQSYLINGVRTSS
                     RKGPGKANLFQPAAILDLVVYHNELKHLQRIREFK"
BASE COUNT          874 a          875 c          854 g          752 t
ORIGIN      
        1 cgctcagatc actgtaaaaa gtcagccggg caaaggaacc gccatcaagg tggaattgcc
       61 cctggaaagg gaacagaatt aactttatgg gagcagcaac aaagaaaata caggtaggca
      121 tcgtagacga ccacagtctc ttgcgaaagg cgctggccaa gctggtcggt tccttcgaga
      181 gctatgcggt cctgttcgaa ggagacaacg gaaaggagat caaaaataag atcacccagc
      241 atgtcatccc cgatatcatc atcctcgacg tgaatatgcc cgatatggac ggatatgaaa
      301 cggtccgctg gctgcataag aactatccgc aggtgaaagt acttgcactc tccatgttca
      361 gcgatgaaaa tacgatcatc cgcatgctcc gtcttggcgc caagggctat attctcaaga
      421 atatcgaacc ggaagagctg aagctggcgc tcgattccat catgaaaaag gatttctatc
      481 tatccgaata catttcgggt aagatcatca gtggtctgca taaggacctc ggcaatcctg
      541 aagatcccat cgttctcaat gacaaggaaa aggaattcct gcgtttggtt tgctccgaaa
      601 tgtcctacaa ggacatcgcc gaaaagatgt tcatcagccc gcgtacggtg gacaattacc
      661 gcaacacatt gttcgaaaag ctgaaggtcc gcacccgtgt aggtctggtg atgtttgcca
      721 tcaagaacgg ccttgtcgag atctagtcaa ccccagcaac aaaacgggaa ggaatacgag
      781 attggttatc gtagcgatca ccagtgtcag cgacgtcagc catcccaagg ccaggattcc
      841 cccgaaatcg ctgaaacaaa agatcacgaa acccgccgtc agcaccgacg aggtatagag
      901 gatactgata cccgtcgtat tgatggtatc gatcacggca tcctgcgccg atgcgccctt
      961 ctcctgtttg taattgacca ggaatcggat cgtgatatcg atagcgatgc ccagcgcgat
     1021 gctgaacaca atgacggtcg aaggtcttag acggactccg gcccaaccca tgatccccgc
     1081 cgtaatgatc aggggtatga cgttgggtac cagcgagcag atcaatatcc gaaaattccg
     1141 gaagagtacc agcatacaga cggcgatcag tacgaaggcc cattcgatac tatcctttaa
     1201 tcccttgatg ataaagctgc ttccttccag gtaggtcacg gtggtgccgg tgaattccac
     1261 ccggtatttc gaagaatcga agagctcggc cgttttcttt ttgagatcgt ccagcaccag
     1321 cgggagacgt tgactgccga tgtccgccat attgatgctg atccgcagct tttgctgatt
     1381 agtatccaca aagcttttca ccaattggct cagctggttc ttcctggcct gccgggccgc
     1441 cgtatccccc gaagagctga tataagaaga aagaaaaggc agatctgccg ggttaggaac
     1501 gtcgtagctg ttgctgtccc cgccgtaata tgattgtctc aaaaacttga ctccttcgac
     1561 caacgataac ggccgggcca tttccggctg cgcgctgata tatttggaca gggcctcgac
     1621 gctgtcgatg gttttgagaa gattcgcgcg taatcccttt tttcttttcg tatcgatcac
     1681 gatctccagc ggcatcaccc ctttgaaatt cgtttcgaag aatttcaggt cggtatacag
     1741 ccggtccttc tgcggcagat cgtccacgat gaacccttcc gatcgcagtc tgaacatacc
     1801 ggcgacggaa atcccgatga tcaggacggt aagaagatag atcggacgct ggtggtggag
     1861 ggtccagatc tccagccttt tcagaatagc cgaaagatac cggttctgaa ggtatcgcat
     1921 atgaccggcc ctgggcgccg gcagaaaact gaggaccacg ggtatcagga taaaagagat
     1981 gacgaacagc agcaggatat tgatgcccgc cactaccccg aattctttca gtatggtgct
     2041 tctcgtcagg gcaaagacgc cgaaaccgat ggctgccgtc agattacaga atagcgtcac
     2101 gatccccatc cggccgatca tcacgatcag ggcctcgtgc ttgtcacccg tatcccgata
     2161 ggccgtatgg tatttgttca tgaaatagat acaattggga atgccgatga ccactacgag
     2221 cgtcggggtc aatgcattca gcagcgtgat cttatacccg agcagatcca tagtgccgag
     2281 gctgaatatc actccgatga tcaccacggc cagggaaagc agcatcgcgc cggcactccg
     2341 gaaaaaaagg agcaggatca gcgacaggat gcataccgat ccgagcagga accacgtggt
     2401 ctccgtggaa actttcttag ccatcttggt gcggatgagc ggcagaccgc tgtagtgcat
     2461 atccagcccg gtcctgactg cgaaactatc taccaggctg atgatcgctc cgacggtttc
     2521 gagccgtcgg gccgaattca tgacggcctt gttcacatgt acggccgtaa gccaggtatt
     2581 cgtcttcgag ttgtatagca ggccttcgta gaaaggcaat cccagcaata ccgccttgcc
     2641 gctgtcgatc tgtgtctgcg aaacagggtt cgaggggaat ataggcacag tcttcggctt
     2701 ttgcagggtc gtatccctga ccaggttgac agccgtggcc acgcctaata cgtcatctac
     2761 cccggacacc ttttccaagg catgatccag ggcaatgtat tgtgtaaaaa ccggaagttg
     2821 gaagagcttg tcggtctgta cgccgatgaa caggaggttg ccgtcctcac cgaattgctt
     2881 tctgaattcc tggtaggcct tgtatttggg attatccgtc gggatcgcac tgttgaaatc
     2941 atagctgagc tggacacggg aagcatgata ggccatgaag ccggtgacgg caagtaaaat
     3001 gaacagccat agcaatcgat atttcaggac cagacgcgcg attgattccc acatgataaa
     3061 aattttactt tgcaaataaa cggcaatttg gggacttttg tcccatacct gagcatcatg
     3121 gaactgcaca aaacaaaagg cattgtcctg cggaccgtca aatatgggga gaccagcctc
     3181 atcgtcacca tttttaccga actattcggc gcgcaatcat acctgatcaa tggggttcgc
     3241 acttcttcgc gaaaaggccc cggcaaagcc aatcttttcc aacccgccgc catcctcgac
     3301 ctggtggttt atcacaacga gctgaaacac cttcaacgca tccgggaatt caaat
//
LOCUS       DASYSV010000284         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1717409, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000284 DASYSV010000000
VERSION     DASYSV010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717409"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..849
                     /locus_tag="VGQ51_08605"
     CDS             139..849
                     /locus_tag="VGQ51_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9036667.1"
                     /translation="MLKELHYMIVDDEPLAIDVLENYLGRLDATHITRCENAVDAFQA
                     IQNQRFDLIFLDIEMPLLSGIEFLKTVKDPPAIIITTAYREYAVEGFELEVIDYLVKP
                     VSFPRFMKAMEKMLKVSRTLPATNEAPPRPPAPEYLFLKVDRKFVKIQLCDILYIESL
                     KDYVRVKTANRSWITNQSLTEITDKLPSDRFARVHRSFTIAFDKISTIDGNCVDIAGT
                     LIPISREHRQDVFKKLHR"
     gene            complement(869..2482)
                     /locus_tag="VGQ51_08610"
     CDS             complement(869..2482)
                     /locus_tag="VGQ51_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV9036668.1"
                     /translation="MLIPRNSLIVLAFILVSKTSRAQTYHELYRPGFHFSPKEKWMND
                     PNGLVRFDGLYHLFFQFYPGDIVWGPMHWGHATSRDLIHWQQQPIALYPDSLGYIFSG
                     SVVVDSNNTSGLGGAAPVPVAPAAKMPTGKGHHTTVHVTPRETARSSIPLVAVFTQHD
                     TAGEHRHTLDFQRQSIAYSLDKGTTWTKYAGNPVLQNPGIADFRDPNVNWYAKGRKWI
                     MALATKDRITFYSSPDLKDWKKESDFGQDAGAHGGVWECPSLFPMTLKVREMERPYWV
                     LLVSNNPGGPNGGSGTQYFIGQWDGHVFRPLDTVTRWIDYGPDDYAGAVWNNMGRRRI
                     FLGWMSNWEYANQLPTATWRNAMTVPRNLFLQESRSKILLASEPVSELEALTKRKVEF
                     RHLVVDSSTSLTNMIKDTAGRYILDVNGPADGSVAFVFSDAAGERLVFGYNAVKDQYY
                     LDRSGAGRTDFHPGFGGIYTAPRLTSSGNLDLRFVMDRSSLEVFADGGLTVMTGVFFS
                     QQPLTRVRVNTTGPWAIKRLSYRALAAIW"
     gene            2518..>2695
                     /locus_tag="VGQ51_08615"
     CDS             2518..>2695
                     /locus_tag="VGQ51_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV9036669.1"
                     /translation="MTLFIASLNSGSNGNCYYIGNEEEAVLVDAGLSCRETERRMLRL
                     GLPIGRVKAIFISHE"
BASE COUNT          643 a          781 c          686 g          585 t
ORIGIN      
        1 agaagagacc accggcgaat ccaagggtat cgggctaaaa aatgtccggc gccggctcga
       61 cctgctgtat ggcaagcgat actccctgga tattcgtgaa gacaacaggc tttttatcgt
      121 ccatctcaaa atgcccgtat gctgaaagag ctgcactata tgatcgtgga tgacgaacca
      181 cttgcgatcg atgtgctgga gaattatctc gggagactcg acgcgaccca tattacacgt
      241 tgcgagaacg ccgtggatgc atttcaggcc atccagaacc aacgttttga cctcatcttt
      301 ctcgatatcg agatgcccct gctctcgggt atcgaattcc tgaagaccgt caaagaccct
      361 ccggccatca tcattaccac cgcgtatcgc gaatatgcag tcgaaggctt cgagctggaa
      421 gtcatcgact atcttgtcaa acctgtatcc tttccacggt ttatgaaggc gatggagaag
      481 atgctcaaag tgagcagaac gctccctgca acgaatgaag ctccgccacg gccgccggcc
      541 ccagaatacc ttttcctcaa agtggatcga aaattcgtca agatacaact ctgcgatatc
      601 ctctatatcg aaagtctgaa ggattatgtg cgcgtaaaga ccgcgaatcg tagctggatc
      661 accaaccaat cgctcaccga gatcaccgac aagcttccct ccgaccggtt tgcccgcgtc
      721 caccgtagct tcaccatcgc ctttgacaag atcagcacca tcgacgggaa ttgcgtcgat
      781 attgcgggta ccctgatccc catttcccgc gaacaccgcc aggatgtatt caagaagctg
      841 catcgctagg cccaaaaaac gggagatttt accagatggc agccagcgcc cgataagaca
      901 gccttttgat ggcccacggc ccggtcgtat tgaccctgac acgcgtcaac ggctgttgtg
      961 aaaagaatac gcccgtcata acggtcaggc ccccgtcggc aaacacctcc agcgagctgc
     1021 ggtccatgac gaaacgaaga tcgaggttgc cgctgctggt gagacgcggc gccgtataga
     1081 tccctccgaa acccgggtgg aaatccgtac gccccgctcc gctgcggtcg agataatact
     1141 gatccttcac cgcattgtat ccgaagacca ggcgttcgcc cgcggcgtca gagaaaacaa
     1201 aagcgacgct tccatcagcg ggaccattta catcgaggat ataacggcct gctgtatcct
     1261 ttatcatatt cgtcaggctc gtgctgctgt ctacgaccag gtgccggaac tcgacctttc
     1321 gtttggtcag ggcctccaat tcggacacag gctcggaagc cagcaatatc ttcgagcggg
     1381 actcctgcaa aaagagattg cgcggtacgg tcatggcatt ccgccaggta gctgtgggca
     1441 attggttggc atactcccaa ttgctcatcc atcccaaaaa gatccggcgg cgtcccatat
     1501 tgttccatac ggcgcccgca taatcatccg gcccataatc gatccagcgc gtgaccgtat
     1561 ccagcggccg gaaaacatgg ccgtcccact gaccgatgaa atactgcgtc cccgatcccc
     1621 cgttgggacc gcccgggttg ttgctcacca gcaacaccca atacggccgt tccatctccc
     1681 gcaccttcag cgtcatggga aaaaggctcg gacactccca aaccccgcca tgcgcgccgg
     1741 catcctggcc aaaatcgctt tcctttttcc agtccttcag atccggcgag gagtagaagg
     1801 tgatacggtc cttcgtcgcc aatgccatta tccatttcct gcctttcgca taccagttga
     1861 cgttaggatc ccgaaaatcg gcgatgcccg gattctgcaa tacgggatta ccggcatatt
     1921 tggtccaggt ggtgcccttg tccaggctat aggcgatgct ctgacgctgg aaatcgagag
     1981 tatgtctgtg ctcacctgcc gtatcgtgct gggtgaatac ggcgacaagt ggaatggacg
     2041 aacgagccgt ttcgcggggc gtgacgtgca ccgtcgtatg atggcccttc ccggtaggca
     2101 ttttggccgc cggcgccacc ggcaccggcg ccgcaccccc gagtccgctg gtattgttcg
     2161 aatccacgac gacgctgccg gaaaaaatat agcccaggct gtcgggataa agcgcgatcg
     2221 gctgctgctg ccaatggatc agatcgcgac tcgtcgcatg tccccaatgc atgggacccc
     2281 aaacgatatc accggggtaa aattgaaaaa aaagatgata tagcccgtca aaacggacca
     2341 gtccattcgg atcgttcatc catttctcct tcggggaaaa atgaaaaccg gggcggtaca
     2401 attcgtgata cgtttgcgcc cgggaggttt tggaaaccaa aataaaggcg agtactatca
     2461 gggaatttcg ggggatgagc atatcgttcc caaaatacaa ttaaatttgc tccatccatg
     2521 actttattca tcgcatccct gaattcgggc agcaacggca attgttatta tattggaaat
     2581 gaggaggaag ccgttctggt cgatgctggt ctttcatgcc gcgagaccga gcgccgtatg
     2641 cttcgccttg gccttccgat cggccgggta aaggcgatct ttatttcgca cgaac
//
LOCUS       DASYSV010000285         7071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_179367, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000285 DASYSV010000000
VERSION     DASYSV010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179367"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2089)
                     /locus_tag="VGQ51_08620"
     CDS             complement(<1..2089)
                     /locus_tag="VGQ51_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEV9036670.1"
                     /translation="MSNSLLDLKALEANRNIVARNYWRDRLKGMEPASYFAHGQPPKA
                     SPASRHYGVCRVVASDEVYQTLCAVAPTDKARHMVLLAGLVILAAKYASAEKVCVFTP
                     GYPGQEETLLIPFLAEDMNRFSFRDFLHTLKERLSADLKYGAYPLEKILATGGWTPAG
                     VPVTGMLVEEFQASGVFDRLSPDILFSFGVSDRLSLRIRYDRNKYEDAPVTQLLHLYL
                     SLLHRLIIGKATRIGEIGLASRDDEERILHQLNDTERNFPLDDPLTEIFRRRAESSGE
                     LPAVESADGHLNFRQLEEASNRLANYLAGDGLGAGEIIALRMERSIDLVVSILAVLKA
                     GCLYLPIDPHYPEHRIRYMLEDSLAQRVLTKESLDEARDRIASCSPLLTEKKSHRWLY
                     VFYTSGSTGRPKGVIGSQKGFINRLRWGWENYPYSDGEAACMKTSIGFVDHFAEIFSP
                     LLQGIPLVIFGEEEILDPARMIAWLSEKRISRITLVPSLLRTLIALIREKNLRIGCLR
                     YVFCSGEALPYSLGKEFYETFGEAVLINVYGSTEVSADATWFETDRAFFAGQDRGYLK
                     RVPIGRPMSNVQVAIVDRTGMLLPPGVAGEILVGGDCVSPGYLNGPELTAAKMIDVPF
                     FKQPGAYLSGDMGRWLANGDIEYLGRIDYQVKIRGIRIRLDEVEQVLVRCPGIGQAAV
                     AVHGKEEDSFLVAY"
     gene            complement(2118..5195)
                     /locus_tag="VGQ51_08625"
     CDS             complement(2118..5195)
                     /locus_tag="VGQ51_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEV9036671.1"
                     /translation="MVFQDVLLESLRRNSDKTAIEENGEATSYAQLLSLSDKVTAFLL
                     ENGVERQTVVGIRLTRRPDLIAAVIGVIRAGGVFVLLDPAMPRRRMEALLAEVRLDHL
                     ITEESWLEVAGRENVAMDADPSHEPGDSLYIYFTSGSTGTPKGIVGRNDSLLQFLRWE
                     IDAFQIEGTVRVSQFISPYFDAFLRDVFVPLLTGGVICVPPADVSFFSPERMVEWIDA
                     NHIRLIHCVPSVWRVVNSGPLHSGLFPDLKYILLSGEKIISSELSGWYEVFGTRIQMV
                     NLYGATETTMIRTCYLIRPEDARTARIPIGEPIYDTALLVAHKDLTPCDTLVPGDLYI
                     ISDYMSAGYLNDPLLTNEKFTWIDMGKEGRKIAFRTGDRARILAGAKIDLIGREDRQI
                     KLRGIRIEPDEIENVLMRSGLVKLAVVMRHTEADGSESLIAFAGRAEGQKQVSKWAEA
                     IETRLREELPAYMVPSDIVEVESIPLLSNGKTDYKALASRLTKSDVIAPVNDTETRLL
                     GIWKEILGDKPISTDDSFHKIGGNSLSIMKLIGRIYKEYNIRISLNDLFNNLNIKKQA
                     DLIRHAKKDLLYVITPAQRKPGYHVSAAQERIYYNQQLNKEAVPYNVPTAWVIEGRPD
                     REQIENAFRRLIERHESLRTEFRIVDGELLQFVKESIPFQLAEAAQQEDDGDIRDVLF
                     RLIRPFDLGAAPLIRCTLIRTLQGKDILLADVHNIVCDGISQAILLSDFYQLYKGVML
                     KPLAIQYKDYAEWEFGFRNTEEYLAHREFWLSAFEGTVPALDLPVSQPLGGDMAGAGG
                     TIQFEIAKRSIEPLLSYLRAEGITPFAGMFSLYLLFLAQLTGQEDIVIGVTSAGRMQQ
                     ELENVLGMFVKALPIRCRLDPGTRFRDLLRDLHRLLIQARSRQVYDITNIVIELNNGR
                     TPAVKSLFNVMFAFQDYLDQWKLEDISVFPLENAGSKFPLTLFVNDWEDCFHFRLEYQ
                     SACFTLQDAELLAGQFADLLLKACAHMDGTVLEIIGAGESQSDLIGDDVAFNF"
     gene            complement(5215..7035)
                     /gene="asnB"
                     /locus_tag="VGQ51_08630"
     CDS             complement(5215..7035)
                     /gene="asnB"
                     /locus_tag="VGQ51_08630"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV9036672.1"
                     /translation="MGRMNDKLIHRGPDAGETLLFDNVALGFRRLSIIGLDNGMQPIH
                     NEDGSIVLICNGEIFNYIELRQQLQRRGHTFRTASDVEVILHLYEDHGMSFLNELNGQ
                     FAFALYDKNKRMLFCARDQLGILPFFYIKTSTAFIFGSEIKAILQHPLVVREVDVVGL
                     DQVMTFAGLISPRTLFKNIHSLENGHYLSVDKAGEVTNLEYWDLLYPEGEIRLNGKTE
                     SRYVDELEELFESSIRLRLRADVPSGLYLSGGLDSSMIAMKVSQVLPGVRKEAFSIDF
                     IDPRHSEYQYQRLVADESNSKLNQMTFLYDDISRRLRHSIYHCECPIKETYNTASLLL
                     SERVRSRGIKVIQSGEGADEFFAGYVGYRFDKLRAMRGIGDETPGQELELRDRLWGDT
                     SFFYEKDFLEFDSVKRALYSKDLNASFGELNCLNHPLIDKRRLHNLDVLNKRAYIDYK
                     IRLVDHLVADHGDRMALANSVEVRYPFLDKDLVEFSTRLPGELKLNGFTEKYILKKMA
                     GRFVPRVISEREKFHFIAPGSPYLLQKRIPYIDDLLSYDLIRRQGFFDPDEVERLKKQ
                     YAQPEFAINAPYETDVLIIVITFGILLEEFFDGPHAGSIQ"
BASE COUNT         1581 a         2044 c         1839 g         1607 t
ORIGIN      
        1 aataggccac taaaaagctg tcctcctctt ttccatgaac ggccacggcg gcctggccga
       61 tgccgggaca gcggaccagc acctgctcca cttcatccag ccgtatccgg atcccccgga
      121 tctttacctg gtagtctatc cggcccaggt actcgatgtc gccatttgcg agccatcgac
      181 ccatatcccc ggacagatag gctcccggct gtttgaaaaa aggaacgtcg atcattttcg
      241 ctgccgtcaa ttccggccca ttgaggtaac cgggactcac gcagtcccca cccaccaata
      301 tttccccggc gacgccgggc ggtagcagca tgccggtcct atcgacgatg gctacctgca
      361 cattggacat cggcctgccg atgggcacgc gtttcagata cccccgatcc tgcccggcaa
      421 agaatgccct gtccgtctcg aaccaggtgg catcggcgct cacttcggtg gacccataaa
      481 cgttgataag gacggcctct ccgaaagtct cataaaattc cttacccaac gaatagggca
      541 gcgcttcgcc gctgcagaaa acataacgca gacacccgat acgcaggttc ttttccctga
      601 tcaacgcgat cagggtgcga agcagcgacg ggacgagcgt gatcctgctg atccgctttt
      661 ccgacagcca ggcgatcatc ctggccggat cgaggatctc ctcctccccg aagatgacca
      721 atggaatacc ttgcaataac ggcgaaaata tttcggcgaa atggtcgaca aaaccgatgc
      781 tggttttcat acacgctgct tcgccgtcgc tgtaaggata attctcccat ccccatcgga
      841 ggcggttgat aaaccctttt tgtgatccga tgacgccctt cggccgtccg gtagaacccg
      901 aagtataaaa aacatacagc caacggtggc tctttttctc cgtaagcagg ggagaacaag
      961 aggctatccg gtcgcgtgcc tcatccaggg attccttcgt cagtacccgt tgagccaggc
     1021 tgtcttccag catatatcgt atccggtgct ccgggtagtg cgggtcgatg ggcagataaa
     1081 gacatcccgc cttaaggacg gccaggatgc taacgacgag gtcgatggac ctctccatac
     1141 ggagggcgat gatctcgcct gcaccgaggc catcgcctgc caggtagttg gccaggcggt
     1201 ttgaggcttc ttccagttgt ctgaagttga gatgcccgtc tgcggattcc acggccggca
     1261 gttcgccgct tgattccgcc cggcgccgga aaatctccgt caggggatca tccagcggga
     1321 agttccgctc cgtatcgttc aattggtgga ggatccgttc ttcgtcgtcc cgggaagcca
     1381 gtccgatctc gccgatgcgg gtggctttac cgatgatcag ccgatgcagc aggctaagat
     1441 acagatgcaa caattgagtg accggcgcgt cctcgtattt attgcggtca taacggatcc
     1501 ttaaactaag ccggtccgac acgccgaacg agaacaggat gtccggcgaa agacgatcga
     1561 acaccccgct ggcctggaac tcctctacga gcattcccgt gacaggaaca ccggcgggag
     1621 tccatccgcc ggtagccaat attttctcca atgggtacgc tccgtatttc agatcggcgg
     1681 atagccgttc tttcagcgtg tggaggaaat ccctgaaact aaatcgattc atatcttccg
     1741 ccaggaaggg gatcagcagc gtctcttcct gtcccgggta gccgggggtg aacacacata
     1801 ccttttccgc ggatgcatat tttgccgcca ggatgaccag tcccgctaaa agcaccatat
     1861 ggcgcgcttt atccgtgggt gcgaccgcac agagggtttg atatacctcg tcggacgcaa
     1921 caacccggca tacgccgtaa tgccgggagg ccggcgatgc cttcggcggc tgtccatgtg
     1981 cgaaatagga agccggttcc attcccttta accggtctcg ccagtaattg cgcgcaacga
     2041 tattccggtt ggcttccagc gccttcaggt cgagtaatga attggacata taggtcttcg
     2101 gctgaattga gaactgttta aaaattaaag gccacatcat ccccgatgag atcgctttgc
     2161 gattcaccgg cgccgatgat ctccagcacc gtgccgtcca tatgcgcaca cgccttcagc
     2221 agcaggtcgg cgaactgacc cgccagcaat tccgcatcct gcaacgtaaa acaggcggac
     2281 tggtattcca gcctgaaatg aaaacaatcc tcccaatcgt tcacgaataa agtgaggggg
     2341 aatttcgagc cggcattctc cagcgggaat acggagatat cttccagctt ccattgatcc
     2401 aggtagtcct ggaaggcgaa catgacgttg aacagactct tcacggcggg ggtgcggccg
     2461 ttgttcagtt cgatgacgat gttggtgata tcgtatacct gccggctgcg ggcctgtatt
     2521 agcagtcggt ggagatcccg gagaaggtcc ctgaagcggg tgccggggtc gagcctgcac
     2581 cggatgggaa gcgcctttac aaacataccc agcacatttt cgagttcctg ctgcatcctg
     2641 ccggccgagg tgacaccgat tacgatgtcc tcctgacccg tcaattgcgc caggaagaga
     2701 agatacagcg agaacatccc tgcaaagggc gtgattccct ccgcacgaag gtagctcagt
     2761 aaaggttcga tcgatctttt ggctatctcg aattggatgg tccccccggc gccggccata
     2821 tccccaccca ggggttgcga gacaggcaga tcgagggcgg ggacggtccc ctcaaaagcg
     2881 ctgagccaga attcccggtg cgcaagatac tcttccgtat tcctgaaacc gaattcccac
     2941 tccgcatagt ccttatattg tatcgcaagc ggtttcagca taacgccttt gtatagctgg
     3001 tagaaatccg acagaaggat ggcctgtgaa atcccgtcgc aaacgatatt atgcacgtcc
     3061 gccagcagga tgtccttgcc ctggagagtc ctgatcagcg tacaacggat caatggtgcc
     3121 gcacccaggt cgaaaggacg gatcagccgg aaaaggacgt cccggatgtc tccgtcgtcc
     3181 tcctgctgcg ctgcttccgc aagctggaag ggtatgctct cctttacgaa ttggagcaac
     3241 tcgccgtcga cgatcctgaa ttccgtcctc aacgactcat gtctttcgat cagccggcga
     3301 aatgcatttt ctatctgctc gcgatccggc cgaccctcga taacccatgc ggtcggcaca
     3361 ttgtacggga cagcctcctt gttgagctgc tggttataat agatccgttc ctgcgcggcg
     3421 gaaacatgat aaccgggctt gcgctgcgcc ggcgtaataa catacagcag gtcctttttt
     3481 gcatggcgga tcaggtctgc ctgctttttt atattgagat tattgaacag atcattcagc
     3541 gagatgcgga tattgtattc cttatagatc ctgcctatga gcttcatgat actcagggaa
     3601 tttccgccga tcttatgaaa gctatcgtcg gtggaaatag gtttatcacc caggatctct
     3661 ttccagatgc ccaatagtct cgtttcggtg tcattgacag gcgctatcac atccgatttc
     3721 gtcagccggg aagccagggc tttataatcg gtcttcccgt tggataacaa ggggatgctt
     3781 tccacttcta cgatatcgga gggtaccata taggcaggca gctcttccct gagacgggtc
     3841 tcgatagctt cggcccattt cgaaacctgt ttttgcccct cggcccttcc cgcaaatgct
     3901 atcagcgatt ccgatccgtc ggcttccgta tgccgcatca cgacggccag ctttaccagc
     3961 ccgctgcgca taaggacgtt ttcgatctcg tccggctcga tccttattcc acgcagcttt
     4021 atctggcggt cctccctgcc tatcagatcg atcttcgctc ctgcaagtat cctggctctg
     4081 tcgcccgtcc tgaaggctat cttcctgcct tccttaccca tgtcgatcca agtgaatttt
     4141 tcgttcgtca gcaaaggatc attcaggtat ccggcgctca tatagtcgct gattatataa
     4201 agatcgccgg gcaccaaagt gtcgcaaggc gtcagatcct tatgtgcaac gagcagggcg
     4261 gtatcgtata tgggctcgcc gatcggtatc ctggcggtgc gggcatcctc cggccggatc
     4321 aggtaacagg tgcgtatcat ggttgtttcc gtcgcgccat aaaggttcac catctgaatg
     4381 cgggtgccga atacctcgta ccatcccgat agctcggaag agatgatctt ttcacccgag
     4441 agaaggatat acttcaaatc gggaaaaaga ccggagtgga gaggcccgct gttaactacc
     4501 cgccaaacac tgggcacgca atggatcagc cggatatgat tggcatcgat ccattccacc
     4561 atgcgctcgg gagaaaagaa ggacacatcg gccgggggaa cacatatgac gccaccggta
     4621 agcagcggca caaagacatc ccggaggaag gcgtcgaaat aggggctgat gaactggctc
     4681 acccgcactg tcccctcgat ttgaaacgcg tcgatctccc accgcaggaa ttgaagcagg
     4741 ctatcattcc ttcccacaat gcccttcggg gtccccgtgg atccggaggt gaaatagata
     4801 tacaggctgt ccccgggttc atgggaaggg tcggcgtcca tcgcgacgtt ctctctgccc
     4861 gccacctcca accagctctc ctcggtgatc agatgatcca gccggacctc ggccaacagc
     4921 gcctccatcc tgcgccgggg catagcaggg tccagcagga caaagacgcc tcccgcccgg
     4981 atgaccccga tgacggcagc gatcaggtcc gggcggcgtg tcaggcggat accgaccacc
     5041 gtttgtcttt ccacgccgtt ttccaacagg aaagcggtta ccttgtccga tagggaaagc
     5101 aactgcgcat aggaggtagc ctccccattc tcttcaatag cggtcttgtc gctattgcgc
     5161 cgcagactct ccagcaatac gtcttgaaat accatgatcc gaatgattta acgattattg
     5221 aatagacccc gcatgaggtc cgtcgaagaa ctcttccagc aggatgccga aagtaatgac
     5281 gatgatcagg acatcagtct catacggggc gttgatggcg aattccggtt gcgcatactg
     5341 cttttttagc cgctcgacct cgtccggatc gaaaaacccc tgccggcgga tgagatcata
     5401 ggaaagaaga tcgtcgatat agggaatacg cttctgcaac aggtaaggac tgcccggagc
     5461 gataaagtgg aacttctctc tttcggagat caccctggga acgaacctgc ctgccatttt
     5521 cttgaggata tatttttccg taaacccgtt gagtttgagc tcgcccggca gccgggtgga
     5581 aaactccacg agatccttat ccaggaaagg ataacgcact tcgaccgagt ttgccagcgc
     5641 catcctgtcg ccatggtcgg cgacgagatg atccaccagc cggatcttat aatcgatgta
     5701 agcccgcttg ttcaatacgt ccaggttgtg cagcctccgc ttatcgatca gcgggtggtt
     5761 cagacagttc aactcaccga aggaagcgtt aaggtccttc gaatataggg cgcgtttgac
     5821 gctgtcgaat tccaaaaagt ctttctcata aaaaaatgaa gtatctcccc atagcctgtc
     5881 ccgtaattcc aactcctgtc cgggcgtctc gtccccgatg ccacgcatcg ccctgagctt
     5941 gtcgaaacga tagcccacat aacccgcaaa gaattcatcg gctccttcgc cggactggat
     6001 cactttgatg cccctggatc gcactctctc cgataagagc aacgaggccg tattatacgt
     6061 ttcctttatc gggcactcgc agtgatagat ggaatgccgg agcctgcggc tgatatcatc
     6121 gtataaaaag gtcatttggt tgagcttcga attgctttca tcggccacca gccgctgata
     6181 ttggtactcg gaatgccgcg gatcgatgaa atcgatggaa aaagcctcct ttctcacgcc
     6241 cggcaggacc tggctgacct tcatcgcgat catcgaagaa tccagcccgc cgctcagata
     6301 gagtcccgat ggtacgtcgg cccttagccg caaccgtatc gatgattcga acagctcttc
     6361 cagctcatct acgtaccggg actccgtttt cccgttgagg cggatctctc cttccggata
     6421 caggagatcc caatattcca ggttggtcac ctctcctgcc ttgtccacgc tcaggtagtg
     6481 accgttctcc aggctatgga tgttcttaaa aagcgtcctc gggcttatca atccggcaaa
     6541 ggtcatcacc tggtcgagcc cgaccacatc cacctcccgc actaccagag ggtgttgcag
     6601 aatggctttt atctccgacc caaaaataaa cgcggtgctt gttttgatgt agaagaaagg
     6661 cagtatcccg agttgatcgc gggcgcaaaa aagcatccgc ttgttcttat cgtaaagggc
     6721 aaaggcgaat tggccattca gctcattgag gaaactcatg ccgtgatcct catataggtg
     6781 gaggatcacc tcgacatcgg atgccgtcct gaacgtatgc ccgcgacgct gtaattgttg
     6841 cctgagttcg atatagttga atatctcgcc attgcagatc agtacgatgg agccgtcctc
     6901 gttatggatg ggctgcatgc cgttgtccag cccgataatg ctgagccgtc tgaaccccag
     6961 ggccacattg tcgaatagca aggtctcccc ggcatccggc ccgcggtgaa tgagcttgtc
     7021 attcatcctg ccgatgacgc ccgcatcgcc gttaccgggc tgtccgtcac a
//
LOCUS       DASYSV010000286         6009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1718189, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000286 DASYSV010000000
VERSION     DASYSV010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718189"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /locus_tag="VGQ51_08635"
     CDS             <1..1934
                     /locus_tag="VGQ51_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV9036673.1"
                     /translation="DDNAVLETQGRYNCKLDGKMLSGSPHRLHLTPGHHTIEIKIYNQ
                     TSPPALRIDGRYLHTDDNWSVTFEDKEWIDASGKASDKSGTVWMPAEELPGEDPAHWH
                     LAVEPHRAVSIEPTAHGMLVDFGKESFGYIDLHGLKGSGGLSLYYGESREEAQCVDSC
                     EVLDRMDIVAPHATDTVLAGSRAFRYVNIQWTGDIRVDSVSMLFEYAPLAQRGSFRCS
                     DPEIDRIWDVAAYTFHLNTREFFIDGIKRDRWIWSGDAYQSYLMNYYLFFDSATVKRT
                     LWALRGKDPVTSHINTIMDYSFYWFMGIYDYYRYTGDSAFIRLCYPRMVSLMNWIGGR
                     RDSRGWLQGLPGDWLFIDWADGLTKKGELSFEQLLYIRSLETMALCASIADDNHGADG
                     YRLLAKDLRARLFVDFWDPARQVFVHDRIDGVRGRDVKRYTNMFGIFFGYLDSAQRSS
                     VKRNVLLNDSIQQITTPYMQFYELEALCAMGQQKQVLRRIRDYWGGMLSLGATSFWEK
                     YDPAEKGAAQYAMYGRPFGKSLCHAWGASPIYLLGKYYLGIRPLSPGYASYAVEPVLG
                     GLEWMEGEVPTPHGNIKLYCSRSRMKVHTVTGEGVLRFSSRTRPVCKEAGIRATGGHG
                     YEITLDPNKDYTINYAAID"
     gene            1918..5283
                     /locus_tag="VGQ51_08640"
     CDS             1918..5283
                     /locus_tag="VGQ51_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEV9036674.1"
                     /translation="MRLSISIFLLFSYAASAQPTPSGFLHPPDSARPWVFWYWMHGAV
                     SREGIRADLVAMKSAGIGGAYLMPIQGPSNPPVYTPVAEQLSPRWWELVRYSMQEADS
                     LGLRLAMHACDGFAVAGGPWITPALSMQKVVWTRTGVEGGRKVSLTLPQPEAIVGYYE
                     DIAILALPTPADWDRTLTPTVTTSKGDARFLATPGNKESFKSDDPCWIQYAYDRPFTC
                     RSIVIHSVNNYQAERLLVEVSDDGNHFRSLGHLTPPRHGWQDGDADITNSIGTVTARF
                     FRFVYDKTGSEPGSEDLDAAKWKPTLKLTGIELSAWPAINQFEGKTGAVWRVSLPSTR
                     QHLPDALCIDPHAIIDLSRNVDAQRHLDWQAPPGHWTILAIGHTSTGHVNATGGGGKG
                     LECDKLNPEAATIQFDHWFGEAFRQAGPELAGRVLKVFHVDSWECGSQNWSPVFRAEF
                     RRRRGYDLINWLPAMAGIPIASADSSERFLHDVRQTIAELLHDNFYGTMARLAHAHGC
                     SFSAECVAPTFTSDDLLHYSETDIPMGEFWLRSPTHDKPNDILDAISGAHIYGKNIVQ
                     AEAFTELREAWDESPAMLKPLQDRNYALGINRLVFHVFVHNPWVAGDRLAGGQGGGTG
                     RRPGMTLGGVGTYLQRDQTWWPQARPWVDYTQRCQWLLEQGRPVADIAVFTGEELPRR
                     AVLPERLVSTLPGLFGSARVESEALRLRNDGEPVTKVPNGVSHGANMADPADWLDPLH
                     GYAFDSFNPDVLLRLATVRDGRIELPGGASYALLVMPGATPMDPDPGAVSAAVAQRLL
                     QLVLDGATVLIDPATSYHSNTLEHMHAEDSIVRAVFHRLLSGQPVGKGRVIRGPWRAA
                     TLDGLGIPRDLSANTAAITYTHRTAGGMDIYFVSNQSSYPRSAEISLRVTGRIPERWD
                     PVTGDIDTLLDWRVEKGRTLVHCGIAPEGSFFLVFRRPAPLFGNRRIAIGNNGVTSVS
                     GPWTVQFDTAYGGPVAPAMFDSLTDWTRNADSAIRYYSGPAVYSTQFQWTQTAGHIFL
                     DLGSVHDIATVEVNGIDCGTTWCGNHPDITRALHPGVNLLRITVTNTWANRLTGDQRL
                     PETHRRTWTAVPFHSDGKLLTAGLLGPVRIVVR"
     gene            5343..>6009
                     /locus_tag="VGQ51_08645"
     CDS             5343..>6009
                     /locus_tag="VGQ51_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV9036675.1"
                     /translation="MEHDFGSMAALIGDPVRARILWILFGGRAYTASELAACVDTSPQ
                     NLSMHLAKLIKADLLSVEVQGRHRYYSFSRPEVAYAVESLAGLLPRKPGASQAAEKED
                     PPIRVCRTCYDHLAGSVGVAVTESLVGMGTIRLSGRTFEVTEKGDRWFDAIGIDCAEL
                     RAQRRGFARACLDWTERRPHLAGSLGAALLQVMMDRQWLRRMEGTRAVLVTGKGRKAM
                     SEYF"
BASE COUNT         1222 a         1666 c         1831 g         1290 t
ORIGIN      
        1 ccgacgataa cgcggtcctg gaaacacaag gccgatataa ctgcaagctc gacggcaaaa
       61 tgctaagcgg ctcgccccac cggttgcatc tgaccccggg ccatcatacc atcgagatca
      121 aaatatacaa ccaaacctct ccgcccgcat tacgcatcga cggccggtac cttcataccg
      181 atgacaactg gtccgtcact ttcgaggata aggaatggat cgacgcgtcg ggaaaggcgt
      241 cggataaatc cggcaccgtc tggatgcccg ctgaggagct acccggcgag gaccctgcgc
      301 attggcatct cgcagtcgaa ccgcatcggg ccgtcagcat cgaaccgacg gcccatggca
      361 tgctcgtcga tttcggaaaa gagtccttcg ggtatatcga tctccatggt ctaaagggat
      421 ccggcggact ttcgctatac tatggcgaat cacgcgagga agcgcagtgc gtcgacagct
      481 gtgaagtact agaccggatg gacatcgtcg caccgcatgc gaccgacacc gttctcgccg
      541 gatcgcgcgc tttccggtat gtgaatatcc aatggaccgg cgatatccgg gtcgactcgg
      601 tatcgatgct gttcgaatac gctccgctgg ctcagcgggg cagtttccgc tgttcggacc
      661 ccgagatcga taggatctgg gacgttgccg cctacacctt ccatctcaac acccgggagt
      721 tctttatcga cggtatcaaa cgggacagat ggatatggtc gggagacgcg taccagagct
      781 acctgatgaa ctattatctt ttcttcgaca gcgccacggt aaagcgtaca ctttgggcat
      841 tgcgcggcaa ggacccggtg accagtcata tcaatacgat catggattac agcttctact
      901 ggttcatggg tatctatgac tattaccgtt ataccggcga cagcgcgttc atccggctat
      961 gttatccccg gatggtgagc ctgatgaatt ggatcggggg acgacgcgat tcccgggggt
     1021 ggttgcaggg attgcccggc gactggcttt ttatcgattg ggccgacggg ttgaccaaaa
     1081 aaggggaatt gagcttcgag caattgttgt atatccgcag tctcgaaacg atggcacttt
     1141 gtgcgtctat cgcggatgac aaccacgggg ctgacggata ccgtctttta gcgaaagact
     1201 tgcgcgcgcg tttgttcgtt gatttctggg accctgcacg gcaggtgttc gtacacgacc
     1261 ggatagatgg tgttcggggc cgcgacgtca aacgctacac caatatgttc gggatctttt
     1321 ttggctatct cgactccgcg caacggtcgt cggtcaaacg gaacgtattg ctcaacgaca
     1381 gcattcagca gataaccacc ccgtatatgc agttttatga gctggaagcg ctgtgtgcca
     1441 tgggacagca aaaacaggtg ctacggcgta tcagggacta ctggggcgga atgctatccc
     1501 ttggtgctac gtctttctgg gagaaatacg atccggctga aaagggtgcg gcgcagtatg
     1561 cgatgtacgg acggccattc ggcaagagtc tttgtcatgc ctggggcgcc agcccgatct
     1621 atctgctcgg taaatattat ctcggcatac gccccttgtc accgggatat gcgagctatg
     1681 ccgtcgaacc tgtcctcggt ggattggaat ggatggaagg tgaggtaccg accccgcatg
     1741 ggaatataaa actatattgc agccgaagca gaatgaaagt gcatacggtg acgggagaag
     1801 gggtgcttcg cttttcgagc aggacgcgac ctgtatgtaa ggaggccggc attcgggcca
     1861 ccggcggcca cgggtacgaa atcactcttg accccaataa agattatacg atcaactatg
     1921 cggctatcga ttagcatatt ccttctgttt tcttatgcgg cgtcggcgca gccgacgccg
     1981 tccggttttc ttcatccgcc cgattccgca cgtccctggg tattctggta ctggatgcac
     2041 ggggcagtct cgcgcgaagg catccgtgcc gatcttgtcg ccatgaagtc cgccggtatc
     2101 ggcggcgcct acctgatgcc cattcagggc ccttccaatc cgcctgtcta tacccctgtc
     2161 gccgaacagc taagtccccg ctggtgggaa ctggtccgtt attcaatgca ggaggccgac
     2221 agtctcggtc ttcgactggc gatgcacgcc tgcgacggct tcgccgtcgc aggcggtccc
     2281 tggatcacgc cggcgctttc gatgcaaaaa gtggtatgga cgcgtaccgg cgtcgaagga
     2341 ggacgaaagg tgagcctcac acttccgcag ccggaggcca tcgtgggcta ttatgaagat
     2401 atcgccatcc tcgctcttcc cacgcccgcc gactgggaca ggaccctcac acccaccgtc
     2461 acgaccagca aaggcgatgc gcgattcctg gcgacgccgg gtaataagga gtccttcaag
     2521 agcgacgacc catgctggat acaatatgca tacgacagac cctttacctg ccgcagcatc
     2581 gtcattcata gcgtgaacaa ttaccaggcg gagcgtttat tggtagaagt tagcgatgac
     2641 gggaatcatt tccggtcgct gggtcatctg accccgcccc ggcatggatg gcaggacggc
     2701 gatgccgaca taacaaattc gatcgggacc gtaacggccc gtttcttccg gttcgtctat
     2761 gataagaccg gatccgagcc cggcagcgaa gacctcgacg cagccaaatg gaaacccacg
     2821 ctgaagctga cgggtatcga actctcggcg tggccggcga tcaatcagtt cgaaggtaag
     2881 accggcgccg tctggcgggt cagtcttccg tcgacacggc agcatctgcc cgatgcgttg
     2941 tgcatcgatc cgcatgcgat catcgatctc agccgtaacg tagacgcgca gagacatttg
     3001 gactggcagg cgcctcccgg tcactggacg atactggcta taggtcatac ttcaactgga
     3061 catgtcaatg ccacaggagg tggaggtaag gggctcgaat gcgacaagct caatcccgag
     3121 gccgcgacca tacagttcga tcactggttc ggcgaagcct ttcggcaggc cggccctgaa
     3181 ctggccggac gggtattgaa ggtcttccat gtcgacagtt gggaatgcgg cagtcagaac
     3241 tggtcgcccg tctttcgcgc cgaattccgt cggcgccggg gatatgatct gataaactgg
     3301 ctgcccgcga tggcgggtat ccctatcgcc tccgccgaca gcagcgagcg cttcctgcac
     3361 gatgtccggc agacgatcgc ggaattgttg cacgataatt tttacggcac gatggcgcga
     3421 ctcgcgcatg cgcatggttg cagcttcagc gcggaatgcg tggcgccgac cttcaccagt
     3481 gacgacctgt tacattacag cgaaaccgac attcccatgg gtgagttctg gctgcgcagc
     3541 ccgacgcatg acaagccgaa tgatattctc gatgcgatat cgggagcaca tatttatgga
     3601 aagaacatcg tacaggccga ggcctttacc gaattgcggg aagcctggga tgaatcgccg
     3661 gcaatgctga agccattaca ggaccgcaac tatgcgctgg gcatcaaccg gctggtcttc
     3721 catgtttttg tccataatcc gtgggttgca ggggaccggc tggcgggagg gcagggcggt
     3781 ggcaccggtc gccggcccgg tatgacgctc ggcggggtcg gcacttatct gcaacgcgat
     3841 cagacctggt ggccgcaggc ccggccctgg gtcgactata cccagcgttg ccagtggctg
     3901 ctggagcagg gtcgtccggt ggcggatatc gccgtattta cgggggagga attgccccgg
     3961 cgtgcggtgt tgccggagcg gctggtatcg acactgcccg gactattcgg gtctgcccgg
     4021 gtggaaagcg aggcgctgcg attgcgcaat gatggcgagc cagtgacaaa ggtgcccaac
     4081 ggcgtgtctc acggcgcgaa tatggccgac ccggcggatt ggctcgaccc gttgcatgga
     4141 tatgcattcg attctttcaa tcccgatgtc ttgctgcggc tggctacggt tcgcgacggg
     4201 cgcatcgagc tgccgggtgg cgcctcttat gctctgctgg tgatgcccgg cgcgacgccg
     4261 atggatcccg atccgggggc tgttagtgcc gcggtcgctc agcggctgtt gcagttggtt
     4321 ctcgatggtg cgaccgtgtt gatcgacccg gctacgtcgt atcattccaa tacgctggaa
     4381 catatgcatg cggaggatag tatcgttcgg gccgtttttc accgattgtt gagcggtcag
     4441 ccggttggaa aaggccgtgt gatcaggggt ccctggcggg cggcgactct cgatggtctc
     4501 ggaattccgc gcgatctcag cgcgaatacc gcggctatta cctatactca tcgtactgcc
     4561 ggcggaatgg atatatattt cgtttcgaac cagagtagtt atccgcgcag tgcggagata
     4621 tcactgcgtg ttacgggacg tatccccgaa cgctgggatc ccgtcaccgg cgacatcgac
     4681 acgttgttgg actggcgggt agagaagggg cgcaccctgg tccattgcgg gattgcgcct
     4741 gaaggatcgt tcttcctcgt ctttcgccga ccggcgcctc tattcgggaa tcggcggatc
     4801 gcaatcggga acaacggggt cacctcggtt tcgggaccct ggacggtaca attcgacacg
     4861 gcctacggag gacccgtggc cccggcgatg ttcgattccc tcaccgattg gacccgcaat
     4921 gccgattccg ccatccggta ttattccggt ccggccgttt attccaccca atttcagtgg
     4981 acacaaacag ccggccacat cttcctcgat ctcgggtcgg tccacgatat cgccaccgtt
     5041 gaggtcaatg gcatcgactg tggcaccacc tggtgcggca atcacccgga cattacccgc
     5101 gccctccatc ccggcgtcaa cctccttcga ataaccgtga cgaacacctg ggcgaatcgc
     5161 ctcaccggcg atcagcgtct tccggagacc catcggcgca cctggactgc cgtgcccttt
     5221 cattcggatg ggaaactcct gacagccggg ttgctgggac ctgtgcgtat cgtcgttagg
     5281 taatttaagc taattttgta caaagacagt tcattttaaa ctgaactgta aatgcaaaat
     5341 ttatggaaca cgatttcgga tcgatggctg ccctcatcgg cgaccccgtt cgggcacgca
     5401 tattatggat cttgttcggc ggccgcgcat atacggccag tgagctggcg gcatgcgtcg
     5461 atacatcccc tcagaacctg agcatgcatc tggccaagct gatcaaagcc gatctgctgt
     5521 cggtggaggt gcaggggagg catcggtatt atagtttttc gagaccggaa gtggcgtatg
     5581 cggtggaatc gctggcgggg ttgctgccgc gaaaaccggg ggcctcgcaa gcggccgaaa
     5641 aagaagatcc gccgatcagg gtgtgtcgca cgtgttatga tcatctggcc gggagcgtag
     5701 gggtcgcggt gacggagagc ctggtgggga tggggaccat tcgattgtcg ggccggactt
     5761 tcgaggtaac ggagaagggc gatcgatggt tcgatgctat cggcatcgat tgtgcggagt
     5821 tgcgtgcgca acggagaggt tttgcaaggg cctgtctcga ctggaccgaa cggcgtccgc
     5881 atcttgccgg ctcgcttggc gcggcgttgt tgcaggtgat gatggaccgg cagtggttac
     5941 gtcggatgga gggtacgcgc gcggtgctgg tgacggggaa gggaagaaag gccatgagcg
     6001 agtattttg
//
LOCUS       DASYSV010000287         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_953370, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000287 DASYSV010000000
VERSION     DASYSV010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953370"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VGQ51_08650"
     CDS             <1..690
                     /locus_tag="VGQ51_08650"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV9036676.1"
                     /translation="THGDNVKGKTACYNREVIAEYGSPLIEHAGIAVLKGNLAVDGAV
                     IKPSAATPALMQHRGRAVVFEDIEDYHRRIDDPALDIDATCVMVLKNVGPVGYPGMPE
                     VGNMTLPKKLLEQGVTDMVRISDGRMSGTAYGTVVLHVSPESATGGTLALVRNGDWIE
                     LDVAGRRLHLDVPEEELAQRRKGWAPPAVAGTDRGYVRLYREHVQQSHLGADLDFLRG
                     GSGSEVSRDSH"
     gene            716..2032
                     /locus_tag="VGQ51_08655"
     CDS             716..2032
                     /locus_tag="VGQ51_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HEV9036677.1"
                     /translation="MHLLLVLFGILLLVALISWVKVNAFLAFLFVSVITGLLLGIPFD
                     HIAGSVYRGIGDTLGQLAIVIVLGAMLGKIVAESGAAQRIATTMMRIFGVRYLQWALM
                     VTGFVIGIPLFYNVGFVLVIPLIFSVSYRYKLPAVYIGVPMLASLSVTHGYLPPHPAP
                     SALVVQFHANMGKTLLYGMAIAVPAVILAGPVYARTLKHIVARPLSGLVAAEKPEAKL
                     PGIFNSLFTALLPVILLMLSTLIPLLFTVPDQWKGVVAFLGDPSVILLVAIATATYTL
                     GLRCGMSMPKITRVYEEAVKDIAVILLIVAGSGALKEVFSDSGVSREIAVVLQDWPIH
                     PLVLGWLMAAIIRVCLGSATVAGLTAAGIILPLTTRMHVDPNLMVLSIGAGSLFFSHV
                     NDSGFWMFKEYFNLSIRDTIRSWSLMESIVSMTGLAGVLVLNAVLG"
     gene            2225..3082
                     /gene="hisG"
                     /locus_tag="VGQ51_08660"
     CDS             2225..3082
                     /gene="hisG"
                     /locus_tag="VGQ51_08660"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEV9036678.1"
                     /translation="MSKLKIAIQKSGRLYDESVRLLKDCGIDLKNGVNKLKAECDNFP
                     MEVYFLRDDDIPQYVEDAVADIGIVGENVLYEKNKKAEIVEYLGFGKCRLSVAVGRGE
                     IYEGVENLNGKKIATTYPLLVRQFLDKNGIRAEIHEISGSVEIAPGIGLADAIVDLVS
                     SGSTLFMNGLREVETVLKSQAVLIRNERMGEEERLLLDKFVFRIRSVKKARNNKYILL
                     NAPNEKVPEIIRLLPGMKSPTVLPLAEAGWSSVHSVLSENEFWDIIEKLKEAGAQGIL
                     VVPIEKMII"
BASE COUNT          671 a          694 c         1093 g          777 t
ORIGIN      
        1 acgcatgggg ataacgtgaa ggggaagact gcctgttata accgcgaagt aattgccgaa
       61 tacgggtcgc cgctgatcga gcatgcgggt attgccgtcc tgaaagggaa tctggccgtc
      121 gacggcgccg tcatcaaacc atccgccgcg acgcctgcgc tgatgcaaca ccggggcagg
      181 gccgtcgttt tcgaggacat cgaggactat caccggcgta tagacgatcc tgccctagac
      241 atcgacgcca cctgtgtgat ggtcctgaag aatgtaggtc cggtgggtta tccgggaatg
      301 ccggaagtag gcaatatgac ccttccgaag aaattgctgg agcagggagt gacggatatg
      361 gtacggatct cggatggacg gatgagcggt acggcttatg gtacggtggt attgcatgtg
      421 tctcccgaat ccgcaacagg cgggacgctg gcgttggtaa ggaatgggga ctggatagag
      481 ttggatgtgg ctgggcgtcg gcttcatttg gatgtgccgg aggaggaatt ggcgcagcgg
      541 aggaagggct gggcgccgcc tgcggtggct ggcacggata gggggtatgt tcgtctctac
      601 cgggaacatg tgcagcagtc gcatctgggc gcggatctgg attttttgcg aggggggtcg
      661 ggttcggaag tgagcaggga ttcgcattaa cgggctggaa gcccataaat actttatgca
      721 cttattgctt gtccttttcg gcatcctgct gctggtagcc ctcatctcct gggtgaaagt
      781 gaatgcattc ctggcattcc tgttcgtttc cgtcattacc ggattattat taggtattcc
      841 tttcgaccat atagccggat cggtctacag aggaatcggg gatacgctgg ggcagctggc
      901 gatcgtcatc gtgctgggcg ccatgttggg gaagatcgtc gccgagagcg gcgccgcgca
      961 acggatcgcc acgaccatga tgcggatatt cggggtacga tacctgcagt gggcgctgat
     1021 ggtgacgggt tttgtcatcg gtataccgct tttttataac gtagggttcg tgctggtgat
     1081 accgttgatc ttttcggtgt cctaccggta taagctacct gctgtatata ttggcgtgcc
     1141 gatgctggca tcgttgtcgg tgacgcatgg ctatctgcct ccgcatccgg cgccatccgc
     1201 gctggtggta cagttccatg ccaatatggg caagacgttg ctatatggga tggcgatagc
     1261 ggtgccagcg gtgatcctgg ccggaccggt atatgcaagg acgttgaaac atatcgtcgc
     1321 caggccttta tcgggattgg ttgccgccga gaagccggag gccaaactgc cggggatctt
     1381 caatagtctt tttaccgcgc tgttgccggt tattttgttg atgttgtcta cgctgatccc
     1441 gttgcttttc acggtgcctg accagtggaa gggtgtggtc gcctttttgg gtgatccttc
     1501 ggtgatcctg ttggtggcca ttgcaacggc cacctatacg ctgggtcttc gttgtgggat
     1561 gtcgatgccg aagatcacga gggtgtatga ggaggcagtt aaggatatcg ccgtcatttt
     1621 actcatcgtg gccggatcgg gggcgttgaa agaggtgttt tcggacagcg gcgtgagcag
     1681 ggagatcgcg gtcgtccttc aggactggcc gatacacccg ctggtgctgg gctggctcat
     1741 ggcggcgatc atccgggtgt gtctgggttc ggcgacggtt gcgggtctga ccgccgcggg
     1801 gatcattctg cctttgacca cgcggatgca tgtggatccc aacctgatgg tgctgtctat
     1861 cggcgcaggg agtcttttct tttcgcatgt caatgacagc gggttttgga tgttcaagga
     1921 gtatttcaat ctgtcgatca gggatacgat acggtcgtgg tcgctgatgg agagtatcgt
     1981 gtcgatgacg gggttggcgg gggtgttggt gttgaatgcg gtgttggggt agggggtggg
     2041 cggcggctgg cgatgtaaaa taaattttgg attttggagg ttggcgagta attttggcgg
     2101 cgaaatgaag atcaacagac aacatcatca ccatcatgca tacttatcct gggggtaggc
     2161 cggtggagtt ttgtctaaaa ctataaacta aagggcttac cacaaggtga gcccaatttt
     2221 ttttatgagc aaattaaaga tcgctattca aaaatcgggc aggctttatg acgagagcgt
     2281 caggctgctg aaagattgcg gtatcgacct gaagaatggg gtcaacaagc tgaaggcgga
     2341 atgtgacaat ttcccgatgg aggtttattt tctgcgggat gacgatattc cgcagtatgt
     2401 ggaggacgcg gtggcggata tcggcatcgt gggtgagaac gtcctttatg agaagaacaa
     2461 gaaggccgag atcgtggagt atctcgggtt tggaaaatgt cggttgtctg ttgccgttgg
     2521 gcgcggcgag atctatgaag gggtggagaa cctgaatggg aagaagatcg ccacgaccta
     2581 tccgttgctg gtcaggcaat tcctcgataa gaacgggatc agggccgaga tacatgagat
     2641 cagcgggtcc gtggagatcg cgccgggtat cggattggcg gatgcgatcg tcgacctcgt
     2701 cagcagcggg agcaccttgt tcatgaatgg acttcgtgaa gtggagaccg tgctgaagtc
     2761 gcaggcggtg ctgatccgta acgagcggat gggggaggag gagcggctgt tgctggataa
     2821 gttcgtattc cggatccggt cggtgaagaa ggcgcggaat aacaaataca ttttgctgaa
     2881 tgcgccgaat gaaaaggtgc cggagatcat tcggctgctg ccggggatga agagtcccac
     2941 agtattgccg ctggccgagg cggggtggag cagcgttcac agcgtgctga gtgaaaacga
     3001 gttttgggac atcatcgaaa aactgaaaga ggcgggggcg cagggcatcc tggtcgttcc
     3061 tattgaaaaa atgatcatct gatatggaag ttatcgatat aagagagaca gccgcgcctg
     3121 ctgcgggcgg atggttgaag aggccggtgc tcgatacccg gtcgctggag gaatcggtcg
     3181 cgggtatact ggccgaagtg aggaaggacg gcgacaaagc cgtacgtaaa ttctc
//
LOCUS       DASYSV010000288         3274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_179949, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000288 DASYSV010000000
VERSION     DASYSV010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179949"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VGQ51_08665"
     CDS             <1..311
                     /locus_tag="VGQ51_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036679.1"
                     /translation="VGQHVLTEIKFIYLMSVGGKRPEEVETDEYSSDNAPDPIDTEFR
                     TLPIPIIISIIISPGSEPWKIILIVLFQIVLWHFLCVRVVFCGINNKTYKGENVRKNP
                     "
     gene            complement(352..756)
                     /gene="rsfS"
                     /locus_tag="VGQ51_08670"
     CDS             complement(352..756)
                     /gene="rsfS"
                     /locus_tag="VGQ51_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV9036680.1"
                     /translation="MEQLSILATRNRSSATRLNRNSKIFKTIIKAIQAKKGENIISLD
                     LRKIPEAVADFFILCEAASTTQVKAIADSVEDEVRKNCDELPYKHEGRQTYQWVLIDY
                     VNVVVHVMLPENRRFYKLEEMWSDAPQEEHEG"
     gene            846..1544
                     /locus_tag="VGQ51_08675"
     CDS             846..1544
                     /locus_tag="VGQ51_08675"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEV9036681.1"
                     /translation="MDSTNNYAMALAKTGKAGHGTLVFAHDQRAGKGQRGRNWESTPG
                     ENIVLSAVLEPVAFPPSAAFGLSAAVALACHDLFSRYAGPESVTIKWPNDIYWNDRKA
                     GGILIENQFKGMQWILAIAGMGININQVVFGVAARTPVSLRQISGRTFDVPALARELG
                     DFLDRRYSEWEAGGVQIEAYNRVLYRRGQEVQLKKDNAVFRTKVEGVSPRGELVTRDV
                     LERRFNFGEVEWVI"
     gene            complement(1578..2051)
                     /gene="bcp"
                     /locus_tag="VGQ51_08680"
     CDS             complement(1578..2051)
                     /gene="bcp"
                     /locus_tag="VGQ51_08680"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEV9036682.1"
                     /translation="MTHLHEGDKAPAFTAKDQDGEKHSLADFKGRRLVLYFYPEDDTP
                     TCTIEACNLRDNFSLLKKEGFAIAGVSPDEEKKHKKFETKYSLPFPLLADPDHKIIDK
                     YGVWGEKQLYGRKYMGLHRTTFLIDEKGTIRKIFLKPRSKQHAEEIVEAARAIFK"
     gene            complement(2062..>3274)
                     /locus_tag="VGQ51_08685"
     CDS             complement(2062..>3274)
                     /locus_tag="VGQ51_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036683.1"
                     /translation="SNNPNGIFEATLEEDGRPVIGFQLDHISYDYTRNINAHIDYKTK
                     ETGGPYLQQLFFLQGYPLPSIYSVPSPTETRGGQVPSPEDAQGGSVASRADASAGHKT
                     SDPAALIAGGLPAPDGVLDIGDGRPHHILIRVRDTEDNQTTSSFDVQYAPASTAAPAT
                     PSTEPAPSPAGTKKFYPRMLDGIELPDCAFYLGEHSLYDSAIIGVTQTGDPGSGRKPA
                     ESAKSAGTDIESLDGAVSAAYTIGAPWIPLDAPVLVRLRPLSPAADSLRTEHSTDRIV
                     MVRWNKADKDVQRPEWKDGWASARFREFGHFQLVEDTTPPTIDFIGIKDGADLGKTSR
                     IRIDVRDNLGATGHFRAELDGSWLCFTHDKNLAWIYNFDEHCPRGRHTLLISVEDVAG
                     NTTVKELHFIR"
BASE COUNT          709 a          781 c          940 g          844 t
ORIGIN      
        1 ccgttggtca gcatgtcctt acggaaatca agttcattta tctgatgagc gtcgggggaa
       61 aaagacccga agaggttgaa accgatgagt acagcagcga taatgcccca gatccaatag
      121 atactgaatt taggaccctt cctattccca ttattattag tatcatcatc tcccctgggt
      181 ctgaaccttg gaaaattatt cttatcgttt tgtttcaaat cgtcttgtgg cattttttat
      241 gtgtccgtgt tgttttttgc ggtattaata ataaaactta taaaggcgaa aatgttcgaa
      301 aaaacccctg aaacggcaaa atatcctgtt aaattgtcat taaagggtct tttatccctc
      361 gtgttcttcc tggggggcat cgctccacat ctcctccaat ttgtaaaaac gccggttctc
      421 cggcagcatt acgtgcacca caacattgac atagtcgatc aggacccatt gataagtttg
      481 tctgccttcg tgtttgtagg ggagctcatc gcagttcttt ctcacctcgt cttccaccga
      541 atccgctatc gccttgacct gggtggtact ggccgcctcg caaaggataa aaaaatccgc
      601 cactgcttcc gggatctttc gcagatcgag ggaaatgata ttttcacctt ttttagcctg
      661 tattgccttg atgattgttt taaaaatttt cgagttcctg ttaagccggg tagcgctgct
      721 gcggttgcgg gtagctaata tagaaagctg ttccaaataa tctgtacttt ttgtgagtaa
      781 ttttacgctt tatcaaaaat aggaatcttt tggcgatcgg tcactctttt gttgaattag
      841 aatccgtgga tagcaccaac aactatgcca tggcactggc taaaacaggc aaggctggac
      901 acggcaccct tgtgttcgcc catgaccagc gggcagggaa aggccagcgg ggacgcaact
      961 gggaaagcac accgggtgaa aacatcgtac ttagcgctgt attagagcca gttgcattcc
     1021 ccccttccgc ggcctttggt ctcagcgcag ccgtggcgct ggcttgtcat gatcttttca
     1081 gccgatatgc gggtccggaa tcggtgacga tcaagtggcc caacgacatt tattggaatg
     1141 acagaaaggc aggaggtata ctgatcgaaa accaattcaa gggtatgcaa tggatactgg
     1201 ccatcgcggg aatgggtatc aatatcaacc aggttgtttt tggcgttgcg gcgcgtactc
     1261 cggtgtcctt gcggcagatt agcgggcgga cattcgatgt gccggcattg gctcgggaat
     1321 tgggagattt tctggatcgc cggtattcgg aatgggaggc cggaggagtg cagatcgagg
     1381 cttataaccg ggtgttgtat cggagggggc aggaggtgca gctcaaaaaa gacaatgccg
     1441 tcttccgtac aaaggtggaa ggcgtgtccc cgcggggcga gctggtgacg cgtgatgtgc
     1501 tggaacggcg gttcaacttc ggagaagtgg aatgggttat ttaaagagat cgttcgatcg
     1561 gattcggcag cgggcagtta tttgaagatc gcgcgggcgg cctccacgat ctcttcagca
     1621 tgctgtttgc tccggggttt gaggaatatc ttacggatgg tgcctttttc gtcgatcaga
     1681 aaggtggtcc ggtgcaagcc catgtacttg cggccataca attgtttctc gccccatacc
     1741 ccgtatttat cgatgatctt atgatcgggg tccgccagca gcgggaatgg cagactgtat
     1801 ttcgtctcga acttcttatg ctttttctct tcgtcggggc tgactcctgc gatggcaaag
     1861 ccttcctttt tcaataggga aaagttgtcg cgcagattgc atgcttcgat ggtgcaggtc
     1921 ggcgtatcgt cctccggata aaaatagaga accagccggc ggcctttgaa atcggcgagc
     1981 gagtgttttt ctccgtcctg gtctttggca gtgaaagcag gagccttatc tccctcgtga
     2041 aggtgtgtca ttagcgatgc tttagcgtat aaaatgcaat tcttttacgg tcgtgttacc
     2101 ggccacatct tctactgaga tcaggagcgt atgacgaccg cgcgggcaat gttcatcgaa
     2161 gttatagatc caggcgagat tcttgtcatg cgtgaagcaa agccaggagc cgtccagttc
     2221 ggcgcgaaaa tggccggtcg ctccgaggtt gtcgcggacg tcgatcctga tccgcgatgt
     2281 cttcccgaga tccgctccgt cttttatccc gataaaatct atcgtgggag gcgtggtgtc
     2341 ttccaccagc tggaaatggc cgaactcgcg gaaacgggca gatgcccatc catctttcca
     2401 ttcgggtcgc tgcacgtcct tatcggcctt gttccaacgg accatgacga tgcggtcggt
     2461 ggaatgctca gtacgcagcg aatccgctgc gggcgacagg ggcctgagtc gaaccaggac
     2521 gggggcatcc agggggatcc acggggcgcc gatggtatag gcggcggaca ccgcgccatc
     2581 cagggattcg atgtccgtcc cggcggactt agctgattcc gccggcttcc gaccggaacc
     2641 cgggtcacct gtttgggtaa cgccgatgat cgccgagtca tagagactat gttcgccgag
     2701 atagaaggcg cagtccggca actcgatgcc gtcgagcatc cggggataga atttttttgt
     2761 accggcagga gacggggcgg gttctgttga aggagttgcc ggagcggcgg tcgatgccgg
     2821 cgcatattgt acatcgaatg acgaggtcgt ttggttgtct tcggtatccc taacgcggat
     2881 gagaatgtga tgcgggcggc cgtctccgat atcgagcact ccgtcggggg cgggtaagcc
     2941 gccggcgatc agggccgcgg ggtcggaagt tttgtgtccc gcagaggcgt ctgcgcgcga
     3001 agcgactgac ccgccctgtg cgtcttcggg tgaagggacc tggccgccgc gcgtttctgt
     3061 gggcgaagga accgaataaa tagagggaag tgggtatccc tgtaaaaaaa acagctgttg
     3121 caaatatggg ccgcctgttt ctttcgtttt ataatcgatg tgtgcgttga tgttacgggt
     3181 gtagtcgtag ctgatatgat ccaactgaaa accgatgacg ggccttccgt cttcttccag
     3241 agtggcttcg aagatgccat tcggattgtt cgat
//
LOCUS       DASYSV010000289         7263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_311544, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000289 DASYSV010000000
VERSION     DASYSV010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311544"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..824)
                     /locus_tag="VGQ51_08690"
     CDS             complement(189..824)
                     /locus_tag="VGQ51_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036684.1"
                     /translation="MILVMSGVSATGQVEVRGTVYDESKLFAMPGVSVLGTSGQGTTT
                     DSVGRYHIVLHPDDSIYFSYLGRHTGKLPVSRIVRNYPLDMSLAVRIDSLPLVVVRPR
                     PYRYDSAENRDEYRNVFDYQPEGVIFGGRRNRQMLQLQQRLIEEEQDKYVSYRFNKNL
                     VKKITGLKGTELDTFMKVYRPTYDFIKNCENDYEFYKYIKDAGVYFQSHGQ"
     gene            899..2929
                     /locus_tag="VGQ51_08695"
     CDS             899..2929
                     /locus_tag="VGQ51_08695"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEV9036685.1"
                     /translation="MKALYTPWLSLALMMAACNHSDTAVKPDFLTADIDSTVRPADDF
                     FSFANGGWIKRTPIPPDESGWGLGNLVQEEIYHRLQKVNEDAVAQHAAEGTITQKIGD
                     LWYSGMDSTDVDKQGLEPLKGELGSIQAMGSAKDITAATAAFHLLGVNVLFSDYVAQD
                     DKNSAEMAYQLSQGGLGMPNRDYYFKTDARTVGVRQAYTEFLVGTFRQLGADEAAARK
                     KAEAVFRLETDLAKASRKLADLRDPYANYHKMTVADLARLTPAFDWSLYFQKVGAEHL
                     DSVIVGQPEFYTALNHELTSTPLPVWKDYFAKQLILRSAPYLDSTTYNRYFEYTKSLS
                     GVKASKPRWKRVIDAEQGAIGEALGQLFVKEYFSDTAKKRYSELIENIRSAFHERIEK
                     LTWMSDSTKQRAYIKLAKITKKVGYPDKWKDFTSLKIDRGPFVLNMQHARDWWYHYNM
                     AKLGKPVDRSEWGMSPQTYNAYYNPSNNEIVMTAAGFTIPGMRDNELDDAFVYGYAGA
                     NWIGHEMTHGFDDQGRQYDVDGNLKGWWQPEDSVQFAHRASMIIRQFDEFVPVDTLHV
                     NGSATQGENIADLGGLLIGLDAFRKTDTYKKGEKIGGYTPLQRFFLGYAYGWMGQVRK
                     ESLARQVMTDVHAPERERVNGPVVNVPEFYEAFDVKPGDKMYRPDSLRVKIW"
     gene            complement(2992..3408)
                     /locus_tag="VGQ51_08700"
     CDS             complement(2992..3408)
                     /locus_tag="VGQ51_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036686.1"
                     /translation="MKHVDYLYFNIYNYFYRISLSRQSVNHRIQTMYLFSLGLGGWLL
                     MMESVYLHTIRHSRFTSPIQSTIFAGSIYFLTAAFFNYIFIVRDRDQKIFGKYEEKFM
                     KNPKRKQHFFISLGILILPYLAMLSFSLIFPRHGQI"
     gene            3526..4713
                     /locus_tag="VGQ51_08705"
     CDS             3526..4713
                     /locus_tag="VGQ51_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV9036687.1"
                     /translation="MIGLKHFIYYTTLGLLAGSAASAQGRRSREIGLSIGVMAPGGYD
                     AITDVPGVKVGQVTLIRGDSIRTGVTAVFPHDGNIFQQKVPAAVYAGNGFGKLSGTTQ
                     VQELGNLETPVVLTNTLNVGTAMEAVVAYTLRQPGNEQVASVNAVVGETNDGWLNDIR
                     GMHVRKEDVWQALSDARTGPVQEGCVGAGTGTICFGFKGGIGTASRKLPLSLGGYTVG
                     VLVQTNFGGVLEIDGVPVGKELGKYEYSRELAGSGVGNDAGRDGGKVGGADGSCMIIV
                     ATDAPLDARNLGRLAKRAIMGLAKTGGIASNGSGDYVVAFSTNDSLRIPNLPSQPVLM
                     RADLSNDAVSPLFMAVIEATEEAIVNSLLAATTTKGRAGHVAEALPMDKVLPILRKYN
                     RIK"
     gene            4710..5609
                     /locus_tag="VGQ51_08710"
     CDS             4710..5609
                     /locus_tag="VGQ51_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEV9036688.1"
                     /translation="MKELFVWFNDALVPTRRASLQVGDLSIQRGYAIFDFFKTLDGRP
                     IFLNEHLDRFFRSAERMRLETGLSADVLRGRIDELMEKNQLPDSGVRLTLTGGYAADA
                     YSIDRPNLVISQSPLTVVPTAELPVAIRLVSYPHIRQLPDVKTIDYLMAIWLKPFIRE
                     QGADDVLYHLDGVVSECPRSNVFILTGEDGDGHGGGLSGGLRGGHGGGLSGGRLVTPS
                     RNILHGVIRGKVMELARRQVPVEERDMTLEELYAAKEVFITSTGKHIWPVVNVDGRTI
                     GKGKPGPLTEALNRDLYRCVKGA"
     gene            5729..>7263
                     /locus_tag="VGQ51_08715"
     CDS             5729..>7263
                     /locus_tag="VGQ51_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEV9036689.1"
                     /translation="MEKKSFITKYVFSTDHKVISKQFLITGILWAIVGGGFSVLMRLQ
                     LGFPNATYPFLQHFSHHLFPTGKIDPSTYYQLMTMHGTIMIFFVLTAGLSGTFANLLI
                     PLQVGARDMASPVINMLSYWAFFVSSVIMAASFFVHGGPSGEGWTAYAPLSALKQADP
                     GDQNGMDLWLISLSFFIMSTLMGGINYIATILNMRTKGMSMNRLPLSTWGLGYTAVMG
                     LLSFPVLLSCFILLLLDRNAGTSFFLSDIYLGETGRALSGTGGSAILYQHMFWFLGHP
                     EVYIVIFPAFGMVSEIISVNSRKPIFGYTAMVISMFAILCLSFMVWAHHMFVSGLNPF
                     LGSLFVVFTVMIAVPSAIKVFNWISTLWRGNIRFTPAMLFSIGFVSTFISGGLTGIYL
                     GNAPLDIQLHDTYFVIAHFHLVMGISAVFGMFAGIYHWFPKMFGRFMNPVVGYVHFWL
                     TLPAAYLIFWPMYYEGMAGLPRRYYDFSNWTTFNQFGTLNEFISAIAIVSFAAQLLFV
                     CNF"
BASE COUNT         1600 a         1826 c         2160 g         1677 t
ORIGIN      
        1 gtcgtgaggg tgtctccggt gccggtgctt ccggtgcccg aatgacaaga agaaaccccg
       61 gcggccataa gggtcagcgc cagggtcatg tgtagtagag atttggcagg catagcaata
      121 tgttagtatg acacaatgta ataaaccggg ggaaaaaatc aaaaaaatgt ttgacggcgg
      181 gacgtcgatt actgtccatg gctctggaaa tagacccctg catccttgat atacttgtag
      241 aattcgtagt cgttctcgca gttcttgata aaatcgtagg tgggccggta caccttcatg
      301 aacgtgtcga gctctgttcc tttgagaccg gtaatttttt tgacaagatt tttgttgaac
      361 cggtagctga catatttgtc ctgttcttct tcgatgagac gttgctgcag ctggagcatt
      421 tgtctattcc ggcggccgcc gaagatgacc ccttcgggct gatagtcgaa gacattgcga
      481 tattcatccc gattctccgc ggagtcataa cgatagggcc gtggccgcac caccacgagg
      541 ggtaggctgt cgatgcgtac ggcgaggctc atgtcgagcg gatagttccg gacgatgcgg
      601 ctgaccggga gtttgccggt atgtctgccg agataggaaa aataaataga atcgtccggg
      661 tgcagcacga tatgatatcg tccaacggag tccgtcgtag taccttgtcc ggaggtaccc
      721 agcacactga cgccgggcat cgcgaaaagc ttcgactcgt catagaccgt ccccctgacc
      781 tctacctggc cggtcgcgct cacgccgctc atcaccagga tcatgcatag tatgacgatt
      841 ttttgctcca ggaccttttt gtggtggcaa attagagcta aaaatttata ttcatggcat
      901 gaaagccctt tataccccct ggttgtcatt ggcgctaatg atggctgcct gcaaccattc
      961 cgacaccgcg gtgaagcccg acttccttac cgccgacata gatagcactg tgcgccccgc
     1021 cgatgatttt ttttcgtttg cgaatggagg ctggatcaag cgaacgccta ttcccccgga
     1081 tgagagcggg tggggtctgg gcaatctcgt gcaggaagag atctatcacc ggctgcaaaa
     1141 ggtgaatgaa gacgcggtgg cgcagcacgc ggcggaaggg accatcaccc aaaagatcgg
     1201 ggatctctgg tacagcggga tggattccac cgatgtcgat aaacaggggc tggagccatt
     1261 gaagggcgag ctcgggtcga tacaggctat ggggtccgca aaagatatta ccgccgccac
     1321 ggccgccttt cacctcttgg gagtgaacgt ccttttcagc gattatgtcg cccaggacga
     1381 caagaacagt gcggagatgg cctaccagct gtctcagggc ggtctgggaa tgcccaaccg
     1441 cgactattat ttcaagacgg acgcaaggac cgtgggggtc cggcaggcat ataccgaatt
     1501 tctcgtcgga actttccggc aactgggtgc ggatgaggct gccgcgcgga aaaaggcgga
     1561 agcggtgttt cggctggaga cggatcttgc gaaggcgtcg cggaagctgg ccgatctgcg
     1621 cgatccctat gccaactatc acaagatgac ggtggccgat ctcgcgcgtt tgacaccggc
     1681 tttcgactgg tcgctgtatt ttcaaaaggt tggcgccgaa cacctcgatt cggtcatcgt
     1741 cggtcagccg gaattctata ccgcgctgaa tcacgagctg acgtctaccc cgctgcccgt
     1801 atggaaagac tatttcgcca aacagctgat cctgcgcagt gcgccctacc tggacagcac
     1861 cacttataat cgttatttcg aatatacgaa atcgttgtcc ggcgtaaagg cgtcgaaacc
     1921 caggtggaaa cgcgtaatcg atgccgaaca gggagcgatc ggggaggcac ttggacagtt
     1981 gttcgtcaag gagtatttca gcgacacggc caaaaagcgt tacagcgagc tgatcgagaa
     2041 tatccgttcg gccttccacg agcgtatcga aaagctgacc tggatgagcg acagtaccaa
     2101 acagcgggcg tatatcaagc tggcaaagat caccaagaag gtaggctacc cggacaaatg
     2161 gaaggacttc accagtctga agatcgatcg cggccccttc gtgctgaaca tgcagcacgc
     2221 gcgcgattgg tggtaccatt acaatatggc caaactgggt aaacccgtcg accggtccga
     2281 atggggaatg agtccccaga cctataatgc gtattacaat ccgagcaaca acgagatcgt
     2341 catgaccgcc gcgggtttca ccatacccgg aatgagagat aacgagctgg acgacgcctt
     2401 cgtgtatggt tatgcaggcg ccaactggat cggtcatgag atgacacatg gattcgacga
     2461 ccagggacgg caatatgacg tcgatggcaa tctcaaaggc tggtggcagc cggaggattc
     2521 ggtacagttc gcccatcgcg ccagcatgat catccggcaa ttcgatgagt tcgtgccggt
     2581 ggatacgctg catgtcaatg gcagcgccac gcaaggcgag aatatcgccg atctgggcgg
     2641 actgctcatc ggtctggatg ccttcaggaa gacggatacc tataaaaagg gggaaaagat
     2701 agggggatat acgcctttac agcgattttt cctgggatat gcgtacgggt ggatggggca
     2761 ggtgaggaag gaaagccttg cccgccaggt gatgacggac gtgcatgcgc cggagcgcga
     2821 gcgggtgaac ggacccgtcg tgaatgtacc ggaattctat gaggcgttcg acgtcaagcc
     2881 gggagataaa atgtatcggc cagatagttt gcgggtgaag atatggtagg attgccggcg
     2941 gcctcgggcc tcggggggcg gctgcccggc gtgcggggtg gcggcggccg cttagatctg
     3001 cccgtgcctg ggaaagatga gcgaaaaaga cagcatggcc agatagggca gtatcaggat
     3061 cccaagcgaa atgaagaaat gttgttttcg ctttgggttc ttcatgaact tctcctcgta
     3121 tttcccgaat atcttctgat cgcgatcgcg aacgataaag atataattga aaaatgccgc
     3181 ggtcaggaaa tagatggacc cagcgaagat cgtcgattgt atcggggatg taaaccggga
     3241 atgcctgatg gtgtgcagat agaccgactc catcatcaga agccatccgc caaggcccag
     3301 cgagaacagg tacatcgtct gtattcggtg attcaccgac tgtctcgaca gactgatcct
     3361 gtagaaatag ttgtagatat tgaaatagag ataatctacg tgtttcatag ggacgttttc
     3421 aattttaggg tatcgggttg aggacccggt ccgaagacca tggtccggtc tattaaacgc
     3481 ttggcagggg taaaatcgta ctttgaccca cattttaata atcagatgat tggtttaaag
     3541 cattttatat attatacaac tttagggctg ctcgccggct ccgcggccag tgcgcagggg
     3601 cggcgttccc gggaaatcgg gctctccatc ggggtcatgg ctcccggcgg gtatgacgcc
     3661 attaccgatg tcccgggtgt aaaagtgggc caggtgaccc tcatcagggg cgactctatc
     3721 cggacaggcg tgaccgccgt gtttccgcat gacgggaata tcttccagca aaaggtcccc
     3781 gcggccgttt atgcaggcaa cggctttggc aagctgtccg gtacgacgca ggtgcaggag
     3841 ctgggtaatc tcgagacccc ggtggttctg accaacacgc tgaacgtggg cacggcaatg
     3901 gaggctgtcg tggcctatac gctgcgtcag ccggggaatg agcaggtggc ttccgtgaat
     3961 gcggtggtcg gagagaccaa tgatggctgg ttgaatgaca tccgcggcat gcatgtccgg
     4021 aaggaagacg tatggcaggc attgtccgac gcgcggacgg ggcccgttca ggaagggtgt
     4081 gtcggcgccg gcacaggtac tatctgtttc gggttcaaag gagggattgg caccgcctca
     4141 aggaaattgc cgttgtcgct cggagggtat accgtcggcg tccttgtaca aacgaatttc
     4201 ggaggtgtgc tggaaataga tggtgttcct gtcggaaagg aactggggaa gtatgaatat
     4261 agcagggaac tggcggggag cggcgtgggg aatgacgcgg ggcgcgacgg ggggaaagtg
     4321 gggggtgcgg atggctcctg tatgatcata gtagccacag acgcgccgct ggacgcccga
     4381 aatcttggcc gtctggccaa acgcgccatc atgggacttg ccaaaacagg cggcattgct
     4441 tccaacggta gcggcgatta tgtcgtggcc ttctccacga atgacagcct gcggatcccg
     4501 aacctaccgt cgcaacccgt gctgatgcgc gccgatcttt cgaatgacgc cgtgtcgcct
     4561 ctatttatgg ccgttatcga ggccacggaa gaagctatcg tcaattctct gctggccgca
     4621 acgaccacaa aggggagggc cgggcatgtg gcggaggcgc tgccgatgga caaggtcttg
     4681 ccgatacttc gaaaatacaa ccgcataaag tgaaagaatt atttgtttgg ttcaacgatg
     4741 cattggtgcc gacacgccgg gcttcactgc aggtgggcga tctatcgatc cagcgcggct
     4801 atgccatttt cgattttttc aagacgctgg atggccggcc gatcttcctg aacgagcatc
     4861 tcgaccgatt tttccgtagt gccgaacgca tgaggttgga gacgggactg tcggccgacg
     4921 tgttgcgcgg caggatcgac gagctcatgg aaaagaacca gctgcccgat tccggcgtcc
     4981 ggctgacgtt gacgggaggc tatgcggcgg atgcctattc catcgaccgt cccaatctcg
     5041 tgatcagcca aagcccgctg acagtggtgc cgaccgctga gctgcctgtc gccatccgac
     5101 tggtgagcta tccgcacatc cgccaattgc ccgatgtcaa gaccatcgac taccttatgg
     5161 ccatctggtt gaagccattc atccgcgagc agggcgcgga cgatgtactt tatcatcttg
     5221 acggcgttgt ttccgaatgc cccagaagta atgtctttat acttacgggg gaggatgggg
     5281 atgggcatgg cggcgggctt agcggtgggc ttagaggcgg ccatggcggc gggcttagcg
     5341 gcggccggct ggtgacgcct tcgcgcaata tcctgcatgg cgtcatccgc ggaaaggtga
     5401 tggaactggc acggaggcag gtgccggtgg aagaacggga tatgacgctg gaggagctgt
     5461 atgcggcaaa agaggtcttt atcaccagta ccggtaaaca tatctggccg gtggtgaatg
     5521 tagatgggag gacgatcggt aagggaaaac cgggcccgtt gaccgaggcg ctgaaccgcg
     5581 atctgtaccg gtgtgtgaag ggtgcctgat cgccgcgcag ccctttccgg acgatttttc
     5641 cacatccgga aaaaaattcc ccatgccggc accgggctcc tcacccatcc acccccgggg
     5701 cacaatagtt gagccggctt agtcgcgaat ggaaaaaaag tctttcatca cgaagtatgt
     5761 ctttagcacg gatcataagg taatctccaa acagttcctc atcaccggca ttttatgggc
     5821 catcgtcgga ggcggcttct cggttcttat gcgtttgcag ctaggctttc ccaatgcgac
     5881 gtatcccttc ttacaacatt tctcacatca tctatttccc acgggtaaaa tagacccttc
     5941 tacctactat cagctgatga ccatgcatgg gaccattatg atctttttcg tgctgacggc
     6001 ggggttgagc gggacatttg cgaaccttct gatcccgttg caggtcggtg cgagagacat
     6061 ggcttctccg gtgataaata tgctatcgta ctgggcgttc tttgtaagca gcgtgatcat
     6121 ggcagcctcg ttcttcgtgc atggcgggcc ctcgggggaa ggatggacgg cctatgcgcc
     6181 gctgagcgcg ttgaaacagg ccgatccggg cgatcagaat ggaatggacc tatggcttat
     6241 cagtttgtcg tttttcatca tgtcgaccct gatgggaggc atcaactata ttgccacgat
     6301 cctgaatatg cgtaccaagg gaatgtccat gaacaggctg ccgttgagca cctgggggct
     6361 ggggtatacc gccgtcatgg gattactgtc ctttccggtg ctgttgtcct gtttcatcct
     6421 gctgctgctg gaccgcaatg cgggtaccag cttcttcctt tccgatattt acctgggtga
     6481 gacgggcagg gccctttcgg gcacgggagg aagcgcgatc ctctatcagc acatgttctg
     6541 gttcctggga cacccggaag tatatatagt gatcttccct gccttcggga tggtatcgga
     6601 gatcatcagc gtcaattcgc gtaagccgat ctttggttac acggctatgg tgatctcgat
     6661 gttcgcgata ttgtgtctgt cttttatggt ttgggcacat catatgttcg tgtcgggttt
     6721 gaatcccttc ctggggtcgc tgttcgtggt cttcaccgta atgatcgccg ttccatctgc
     6781 tatcaaggtc ttcaactgga tcagcacgct ctggcgcggc aatatacggt tcacgccggc
     6841 catgctgttc tccatcggat tcgtgagcac ctttatttcc ggcggtctca cgggcattta
     6901 cctgggaaat gcaccactgg acattcagct ccacgatacg tatttcgtga tcgctcattt
     6961 ccatctcgtg atggggatct cggcggtatt cggcatgttc gcggggatct atcattggtt
     7021 cccgaaaatg ttcggccgct tcatgaaccc cgtcgtaggc tatgttcatt tctggctgac
     7081 gctgcctgcg gcctatctca ttttctggcc gatgtattat gaaggaatgg cgggattacc
     7141 gagaaggtat tatgattttt cgaactggac tacatttaat cagtttggca ccttgaatga
     7201 attcatctcg gcgatcgcca tcgtatcgtt cgccgcgcag ctgcttttcg tctgcaattt
     7261 ttt
//
LOCUS       DASYSV010000290        16384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1463781, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000290 DASYSV010000000
VERSION     DASYSV010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 16384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16384
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463781"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VGQ51_08720"
     CDS             <1..1077
                     /locus_tag="VGQ51_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV9036690.1"
                     /translation="MIIGRSANLTAPIPGRTACQFRNRCWEGCPFGGYFSTQSSTLPA
                     AMATGRLTVRPYSIVTRILYDKDSRKATGVEVLDAETNKTYTYNARVIFVNASALNSA
                     WVLMNSATDVWPDGLGSSSGELGHNVMDHHYNVGAAGVMEGYDDKYYFGRRANGVYVV
                     RFANLFGDKRDYLRGFGYQGGASRDGWSRNVAEMSIGAELKETLCEPGQWQIGMTGFG
                     ELLPYHENKVSLDKNKKDKWGLPVLSMDAELKENEKKMRKDMASEAADMLTAAGVKNV
                     RTWDSGHAVGDGIHEMGTARMGRDPRSSVLNAHNQVWDAKNVFVTDGACMTSSACQNP
                     SLTYMALTARAADFAVSELKKGNI"
     gene            1089..1670
                     /locus_tag="VGQ51_08725"
     CDS             1089..1670
                     /locus_tag="VGQ51_08725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEV9036691.1"
                     /translation="MNRRDALTRVGLLLGGTVVGAEMFLSGCTNSPEKTIGMAGTNFS
                     NDDIAFLDEIAETILPKTDTPGAKDAKVGEFMARIVKDCYTQDEQKTFLDGMKKLSDA
                     SKQQNGRPFLESTPEQRHDLLVALDKEQKAYTASKKEGDPTHYFRMMKELTLWGYFTS
                     EPGATKALRYVAVPGKYEGCIDYKKGDKAWATS"
     gene            complement(1738..2034)
                     /locus_tag="VGQ51_08730"
     CDS             complement(1738..2034)
                     /locus_tag="VGQ51_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036692.1"
                     /translation="MKRVPLFLALCLVSSLACRRKISRDDVKVQLEKAMSHQLEKQQS
                     SNDPHPRFDVLDVAWFEDSAFYLCEFTVKMTLPNGQDTTGVMKKKISKDFNSIE"
     gene            2039..3727
                     /locus_tag="VGQ51_08735"
     CDS             2039..3727
                     /locus_tag="VGQ51_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEV9036693.1"
                     /translation="MKIQEKYSMRSGAACFSFFWLFFSSCGSPAHNADGAPIADSSIR
                     NGKELAMKYCVSCHQLPDPGLLDKASWQKGVLPGMGPRLGIFGYKGVRYPSFANDPHV
                     GRGFYPSAPLMAGWQWQEIIDYYTGTAPDSIPPQPEHLPVEMGLKLFKAMAPGEGGGE
                     AGSVKAGAAGSDKAGASKAGTAGSDETGASKAGTAGARPLTSYVRVDSSGGRRQIIAG
                     TLFPGMLWRYDEQLRLTDSVGLHGGIVDAQWAGDSGIVCNIGNINPNDQLLGTVNRVS
                     YDGKGRLRLDTVPVLAELARPVQATEADLNGDGRKDLLVCEFGNQKGELAWWEKDLNG
                     KYIRHVLRAQPGAIKAYIRDADGDGRPDIWALFAQGDEGIFLYLNKGGGRFEERRVLS
                     FPPEYGSSYFELADFNKDGHPDIVYTCGDNADYSVVPKPFHGVYIYMNDGTNRFTQSY
                     FYPINGCYKAMARDFDGDGDLDIATIAYFADFGKHPEEGFVYLQNEGGMQFKPLTLPE
                     GEAGRWLVMDAGDLDGDGKIDLVLGNFSMGPSLSKGKRDWKKAPAILFLKNVGR"
     gene            3852..7145
                     /locus_tag="VGQ51_08740"
     CDS             3852..7145
                     /locus_tag="VGQ51_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9036694.1"
                     /translation="MRIKTNVACKGHFLAFTLLFFTFSALAQRTVTGRVVSKTDNTPL
                     PLATVALKGSRSATASGQDGTFALKLPGNQASGTLVISAVGFDNLQVPLTGKDNLGDV
                     VLTQANNTLNDVIVTGYTVQKKKDITGAVAIVDISDAKKIPMTSSDQLLQGQASGVTV
                     LNSGGPGAPSAVFVRGVSSFGQTQPLYVVDGAQVPDMTYINPNDIESINVLKDAGSAA
                     IYGVSGGNGVVVITTKRGRQGKATINYDAWFGNQKPLSGNVWNLMNPQQQSSLAFTAG
                     DKPTEALYPGGAGTIPTYGYHGATAFGAFGTAGVTSDANILNAYVFDANNPGNDYLVQ
                     KFATGAGTDWFHSVFKPAFEQQHTLSASGGSDKSAYYLSLNYLNQDGTLMNTYEKRYS
                     ARINTVFNVKNHVRVGENAMVTYRENNGGYNGDQQQEGGSIAFTFREMPIIPIYDVKG
                     HFGGGYDGPSGEPLGNGSNPYAIMSRESTNQAHFVTVEGIAFVEADFLQHFTARMAFG
                     GRLWNQYYWNITYNPYEDYESHANPNSATENEQMSASHNWTNTVTYKNDWGKHSLLIL
                     GGYEQRGTNGREFHASAQSFFSLDPNVVELQYGTPVNAPTSTIWQPTSTESLFGRIDY
                     AYNEKYLIGATIRRDGYSVFYQDKQYGTFPSVSLGWRISEEDFMKDVTWVNNLKLRAS
                     YGVAGNNANIAGNNAYTSFSQGTGASYYSVSGAASSTSPGFFQNQLGNQNVTWERDKM
                     TNIGLDGTLFNNHWDFSIEWYKKAISGLLFPADLPATVGGATPPSINIADVQNTGLDI
                     STTYHGRINHDLTFNITANITSYNSLITAEPGSGYFDFGYSRDLDIVRNQVGHPIGAF
                     YGFQTKGLYQSDVDAGKGATYSGAKAGSFIYKDVNGDGVIDATNDRTWLGNPNPKFTY
                     GLNLNAAYKGFDFSMVLYGSYGNKDFNYVKYWTDFYSTFQGGKNLDLYNKAAIVNNGV
                     VTNPGAILPPASYGQAMGSSTVSSFYVEPGSFLKCRIIQLGYTITPSAIKKVGIDKLH
                     FYVQATNPFMITKYSGPDPELLPSLSNNGGSNNQTNVGNRQNAAFGIDYGAYPTNQHV
                     FLFGINASF"
     gene            7166..9037
                     /locus_tag="VGQ51_08745"
     CDS             7166..9037
                     /locus_tag="VGQ51_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9036695.1"
                     /translation="MKKIGKYILISLSLPLSVILIYACTKNLKKPPVGSYGPSNLASK
                     EGVDGLLIGAYSMLDGVANGSTYNGGGYTSWETSTTNWVYGGIASDDAYKGSNTTDQP
                     NAAPIENHSVASSNEYLAEKYSFCYNAIQRANDVIRELPDAAKVDAQVTAAFAAEEAA
                     EARFLRGVFHLEAAKLWRNIPYVTERITYTANNYILPNPGPIWDSIEADFTAAMAVLP
                     KTQSQIGRANYYAAEAFLAKTYMFDHQYTKALPLLTDLITNGTTQSGAKYALGPFEDN
                     FDAAHRNNSESVFCVEMTANDGSGGWNSDQGDILNYAAGPYTGCCGFYQPSYSMGNAY
                     KTDANGLPMFTIDQAANGWPAYDDVNLKNDHGLLASDPFTPATDNLDPRIDWTVGRRG
                     IPYLDWGLCGGETWSRGDIVPYNPIKNVFWHYNMGTTTDPLAQGWAANQGTAINYDMI
                     RFADVILWRAECEVEGNDLVNAQKDVNMVRNRMVAHPEYWVHTYVDNADPSKGFTTTP
                     AANYVIGLYGTVAGTDFVTKGQAYARMAVYTERQLELAMEGHRFFDLQRWDGLYGGPM
                     PKGWMAGVLNAYIKADTRISNPVLNGQKFTAGTNELYPLPQHQVDLEAGKLKQNPGY"
     gene            9107..12682
                     /locus_tag="VGQ51_08750"
     CDS             9107..12682
                     /locus_tag="VGQ51_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEV9036696.1"
                     /translation="MIKIRILVVAAIALCVFSCRKKTQFELIGSAHSGIHFNNTIVEN
                     DSINPLDLTNIYNGGGVGIGDFNNDGLPDIYFTGNTVSNKLYLNKGNLVFEDITDIAG
                     VGGNGEWCRGAAVVDVNNDGLPDIYVCANIKQDPRQRTNLLYVNQGVDAKGIPHFKEM
                     GVEYGLADTLYSTMATFFDYDNDGRIDMYLTVNGIPKGINPNNFRKPVTDGSFPSTGH
                     LYRNEWNDSLKHPVFRDVSKQAGITIEGFGHAATIADINRDGWKDIYVTNDFTGNDIL
                     YINNRDGTFTNKIASYFKHTSANGMGQDIEDVNNDGLADLVELDMNPPDNYRKKMFLN
                     PSSYLFYQNSDQYGYQYQYIRNSLQLNQGPRVEGDSAGDPVFSEISWLSGIAETDWSW
                     TPMLADFDNDGYRDLIVTGGYPKDITDHDFQMFMRQASNIISHEDIMKQIPEVKIHNF
                     AFRNKGDLTFADVSEDWGMMTPGFSNGAAYADLDGDGDLDLVVNNINDEARIYKNMAR
                     ERGGSGDHFLRVVFSGDSLNRGGLGAWVELHYDHGKQQVYENNPYRGYLSTIENAAHF
                     GLGPVRTVDTMLIRWPDGKAQTLYNVACDQVVKTDWRKARDRYVPPGPRVAPAAVFRD
                     ITDSAGIHYRQKEKDYIDFNVQKLLPHKFSQYGPGIAVGDVDGNGLDDLVVGGSANYS
                     AEVFLQQSDGRFVASPLLKDSAAALAKLSDDEGILLFDADGDGDMDLYIAAGGYEHGE
                     NSEAYTDRLYINDGHGHFALAPADALPSHNVTSKFCVRAADYDHDGRPDLFVSGRVDP
                     GHYPQPVTSFIYHNETVDGRVRFVDVTAKVAPGLEKIGMVCDGIWTDIDNDGWPDLVL
                     AGEWMPLTVFKNERGIFRNVTGGTGESGVTGWWNSIVAGDFDNDGDIDYVVGNLGLNS
                     YYKADAEHPVRVYGKDFDNNGVYDMLTSLYLPDQKGRLREFPAIGRDDLLRQMNSMRH
                     RFPSYRDYAVATMDKVLTPEDRNGATILTANQMRSCLFRNDGGGKFTMIPLPVEAQFS
                     VINGMVAGDFDGDGNLDIVMSGNDYGTEVSTGRYDAMNGLLLRGDGSCGFFPATILQS
                     GIFIPGDGKALVALRGAGGHYLLAAGQNRGPLKVFKRRMDGRQVALMPSEVAAMVRLK
                     DGRVRREEYYYGESFLSQSGRFMDWNGPVVEAEIVDEKGRKRVVR"
     gene            12785..13216
                     /locus_tag="VGQ51_08755"
     CDS             12785..13216
                     /locus_tag="VGQ51_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV9036697.1"
                     /translation="MSNFPLWLVYALLSAIFAALTAIFAKIGLKDVNADLATAIRTAV
                     ILLITWGIVLFRGNVAGLPGLTRNNWIFLVLSAIATGMSWLFYYRALQIGQVQQVAVI
                     DKGSIFFTIFLSFFFLKEPLTPKVLLAAGLIFAGMLVLVWK"
     gene            13386..13976
                     /locus_tag="VGQ51_08760"
     CDS             13386..13976
                     /locus_tag="VGQ51_08760"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9036698.1"
                     /translation="MIGIHNEKELLQQVSQGDRDAFRRLYTGYFGHVQRYIYLFEPSR
                     DNLDELTQDVFVRVWEKRGHLGEVESFKDYLFFIARNVVFNYLRGLKVQKKIRELEGL
                     DGTGVYETEELVLFRQYYRIALEAMEKLPPGRRKVLKMSIDDGLSLDEIAAELKISRA
                     GVKKQLYAATAFVRKYLQEHGEITALLFVFLSLFER"
     gene            14318..15358
                     /locus_tag="VGQ51_08765"
     CDS             14318..15358
                     /locus_tag="VGQ51_08765"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEV9036699.1"
                     /translation="MNVKEAQQFVADYANGEFTPTEHAAFLQWVRGAGVRELELIAEE
                     HETHHGQWVFSTEGPSPEWVGALEDRLDRLAEKRVKAPVVRMPFGKRVWIVAASVAIL
                     IIAGTFWYSQTGGSLNKEERQQALATLTQTMKVERGGEQRSVTLPDGSKVWLNVASIL
                     KYPVKFSGPDRVVELSGEGYFEVSQRSDMPFRVLIKDAEVKVLGTHFNIMAYDDEPVA
                     RTSLIDGSVSVESGSQRVTLHPGEQGEIPYSSPGATGPIKVIPGIDPSAVVAWRDGVL
                     KFEGTPMHELLRTLGRCYDVEIQIDQNVPDTPGYGIFSRSQGLDSIMKQLKRDHLQFI
                     TNGKTVRVTMAK"
     gene            15490..>16384
                     /locus_tag="VGQ51_08770"
     CDS             15490..>16384
                     /locus_tag="VGQ51_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEV9036700.1"
                     /translation="MRSNRLAVFLLIACLLNCRPSYSQTDPLITLSEKDVPLQKVLDE
                     IHTKTGYAYFGEGDWPKIAHKVSISVRNASIRKVLDTCFFDQPVLYELDEKERFIFVR
                     LRPKEERKVHGWIVDENRDPVGGASIVARGDVSGVSNDDGEFELSTHFSDSKLSVSSI
                     GYEPQELPLPPSGKDITITLRSRTKALIEAVVLHTGYQDVKRKASTGSFDEVDNDLLN
                     RRVSTNVLDRIDGVTSGVLFNKNIVTSANQSAITIRGRSTIYANPNPLIVVDNFPYPG
                     DINNINPADVESITVLKDAAAA"
BASE COUNT         3922 a         3632 c         5038 g         3792 t
ORIGIN      
        1 atgatcatcg ggcgcagtgc taacctgacg gcaccgatac ccgggaggac ggcttgtcag
       61 ttcaggaaca ggtgttggga ggggtgtcct ttcggtggat atttcagtac gcaatcgtct
      121 acgctgcctg ctgcgatggc cacgggaaga ttgacagtgc ggccttattc catagtgacg
      181 cggatactct atgacaagga ttcgcgaaag gcgacggggg tggaggtgct ggatgcggag
      241 acgaacaaga cgtatactta taatgcccgg gtgatcttcg tcaatgccag tgcgctcaac
      301 agcgcctggg tcctgatgaa ctctgcgacg gatgtctggc cggatggtct gggcagcagc
      361 agcggagagc tggggcataa cgtgatggat catcattaca atgtgggtgc tgcgggtgtt
      421 atggagggtt atgacgataa atattatttc ggcaggcgcg cgaatggggt atatgtagtg
      481 cgttttgcca atctgttcgg cgacaaacgg gattatctgc ggggtttcgg ctaccagggg
      541 ggtgcgagca gggatggatg gtcgcgcaat gttgcggaga tgagcatcgg ggcggagttg
      601 aaagagacgc tgtgtgagcc gggacagtgg caaatcggga tgaccggatt cggtgaactg
      661 ctgccgtatc atgagaacaa ggtttcgctg gataagaata aaaaagacaa atggggattg
      721 ccggtgttgt cgatggatgc cgagttgaag gagaatgaga aaaagatgcg aaaggatatg
      781 gcgtcggaag cggcggacat gcttacggcg gcgggcgtga aaaacgtacg tacgtgggat
      841 agtggtcatg cggtggggga tgggatccat gagatgggaa cggcaaggat ggggcgcgac
      901 ccccgcagtt ccgtgctgaa tgctcataac caggtgtggg atgcgaagaa tgtttttgtg
      961 acggatggag cctgtatgac gtcctcggcg tgtcagaacc catcgctcac gtacatggcg
     1021 ctgacggcaa gggcggcgga ttttgcagta agtgaattga aaaaaggaaa catctaaaaa
     1081 atacaactat gaatagaaga gatgcattga cccgcgtagg tttgctgctg ggcggcactg
     1141 tcgtaggagc cgaaatgttc ttgtccgggt gtacgaattc gcccgagaag accatcggca
     1201 tggcgggcac aaatttctcc aatgacgata tcgcatttct cgatgaaata gcagagacca
     1261 tcctgccgaa gacggatact cccggcgcga aggatgcaaa ggtgggcgag ttcatggccc
     1321 gcatcgtcaa ggactgctat acgcaggatg agcagaagac ctttttggac ggcatgaaaa
     1381 agctgagcga cgcgagcaag cagcagaacg gaagaccgtt cctggagagc acgcccgagc
     1441 agcggcatga tctgctggta gcgctggaca aggagcagaa ggcgtatacg gcgtcgaaga
     1501 aagagggtga tccgacgcat tatttccgga tgatgaagga gctgacgctc tggggttatt
     1561 tcacatccga gccgggagcg acgaaggctt tgcgttatgt tgccgtgccg ggcaagtatg
     1621 aggggtgtat cgattataag aagggagata aggcctgggc gacgagctaa ggaaggatgg
     1681 acgtgcggag gatggagggg agcgaccggc aggaaggagt gaacgatcgg tccccggcta
     1741 ttcgatggaa ttgaagtctt tggaaatttt tttcttcatg acgcccgtgg tatcctgacc
     1801 gttgggcagc gtcattttta ccgtgaattc gcagagatag aaggcggagt cctcgaacca
     1861 ggcgacatcc agtacgtcga aacgagggtg aggatcgttg gaggattgtt gtttctccaa
     1921 ctggtgggac atggcctttt ccagttgtac ttttacatca tccctggata tttttcgccg
     1981 gcaggctagc gacgacacga ggcaaagggc gaggaacagt ggaacccgtt tcatgccaat
     2041 gaagatacaa gaaaaataca gtatgcgcag cggtgctgcc tgtttctcct ttttttggtt
     2101 gtttttttcg tcatgcggtt cgccggctca taatgccgac ggcgcgccga tcgccgactc
     2161 atcgatccgg aatggaaagg agttggcgat gaagtattgt gtgtcgtgtc atcagctgcc
     2221 ggatcccggt ttattggata aggcctcctg gcaaaaagga gtgcttccgg ggatggggcc
     2281 ccgactgggt atatttggat ataagggtgt ccgttatcct tcctttgcca acgatccgca
     2341 tgtcggccgg ggcttttatc cttcggcgcc cctgatggcg gggtggcagt ggcaggagat
     2401 catcgattac tataccggta cggcgccgga ttccatacct ccacagccgg agcatcttcc
     2461 agtcgaaatg ggcctgaagc ttttcaaggc gatggcgccc ggtgaaggag gtggcgaggc
     2521 agggtcggtg aaggctggcg cagcgggtag cgataaggca ggggcatcga aggctggcac
     2581 agcgggtagc gatgagacag gggcatcgaa ggctggcaca gcgggtgcca ggccattgac
     2641 cagctatgta cgggtggact ccagcggagg acggcggcag atcatagccg gcacgctatt
     2701 cccggggatg ctctggcggt atgatgagca attgcgattg accgactccg tgggtttgca
     2761 tgggggtatc gtggatgcgc agtgggcggg cgattcgggt atcgtttgta atatcggcaa
     2821 tatcaatccc aacgatcagc tgttgggtac agtgaaccgt gtgtcttatg atgggaaggg
     2881 gaggttgagg ctcgacaccg tgcctgttct cgcggagctg gcgcggcctg tgcaggcgac
     2941 ggaggcggac ctgaacgggg acggccggaa ggacctgctg gtttgtgagt tcgggaacca
     3001 gaaaggtgaa ctggcgtggt gggagaagga cctgaatggt aagtatatcc gtcatgtgct
     3061 gagagcacag cccggggcga tcaaggccta tatccgggat gccgacgggg atggccggcc
     3121 ggacatctgg gcgttgtttg cgcaggggga tgaggggatc tttctgtatc tgaataaagg
     3181 cgggggtagg tttgaagagc gaagggtatt gagcttcccg ccggagtatg ggtcatcgta
     3241 tttcgagctg gctgatttca ataaggacgg tcacccggac atcgtctata cctgcgggga
     3301 taatgcggac tattcggtag tgcctaagcc gtttcatggc gtgtatatat atatgaatga
     3361 cgggacgaac cggtttacgc aaagctattt ttacccgata aacgggtgtt ataaagcgat
     3421 ggcgcgggat ttcgacgggg atggggatct ggatattgcc acgatcgcgt atttcgccga
     3481 tttcggaaag catccggagg aagggttcgt ctacctgcag aatgagggag ggatgcagtt
     3541 caagccattg acgctgccgg agggtgaggc gggaaggtgg ctggtcatgg atgcggggga
     3601 tctggatggg gatggaaaga tcgacctggt attggggaat ttttcgatgg gaccgtcttt
     3661 gagcaagggc aaaagggatt ggaagaaggc gcccgcgatc ctgttcctga agaatgtggg
     3721 gaggtgaaat tcctggaacc gattgcaaaa gaattgttaa aaaagcatct taaaaggtta
     3781 aaaaagtatt aatttttaac ctatctcatc agtagattag acggacgatt caccattcaa
     3841 atctactttt catgagaatc aaaactaacg ttgcttgcaa ggggcatttc cttgccttca
     3901 ctctcctttt cttcacattc agcgctttag cacagcggac ggttaccgga agggttgtct
     3961 ccaaaacgga caacaccccg cttcctcttg ctacggtcgc gcttaagggc tcacggtccg
     4021 ccaccgcttc ggggcaggac gggaccttcg ccttaaaatt acctggcaat caggccagcg
     4081 gcacactggt gatcagcgcc gtgggctttg acaatctgca ggtgccgctt accggtaaag
     4141 ataacctggg ggatgtggtc ctgacccagg cgaacaacac gttgaatgac gtgatagtga
     4201 ccggttatac ggtgcagaag aaaaaagata tcacaggggc cgttgccatc gtggatatct
     4261 cggatgcgaa aaagataccg atgaccagca gcgatcagct gttacagggt caggcttcgg
     4321 gtgtcacggt cctgaactcg ggcggaccgg gtgctccgag cgcggtgttc gtaaggggtg
     4381 tatccagctt tggtcagacg cagccattgt atgtggtgga cggggcccag gtacctgata
     4441 tgacctatat caacccgaac gacatagaaa gcatcaacgt cctgaaggac gcggggtcgg
     4501 ccgctattta cggggtttcc ggaggtaacg gtgttgtcgt tatcacgaca aagagaggac
     4561 gtcaggggaa ggctacgatc aactatgacg catggttcgg taatcagaag ccgttgagcg
     4621 gaaatgtctg gaacctgatg aatccccagc agcaatcgtc gctggccttt actgccggtg
     4681 ataagcctac cgaggccctg tatccgggcg gagcaggaac gatccctact tatggctatc
     4741 acggtgcgac ggctttcggc gcgttcggta ccgcgggagt caccagcgac gcgaatattc
     4801 tgaatgctta tgtcttcgac gcgaataatc cgggcaatga ttacctggtc cagaaattcg
     4861 cgacgggtgc aggtacggac tggttccatt cggtgttcaa accggccttc gagcagcaac
     4921 atacgttgtc ggccagcggc ggtagtgata agagcgctta ctatttgtcg ctgaactacc
     4981 tgaaccagga cgggacgctg atgaacacct acgaaaaaag atattcggcg cggataaata
     5041 cggtgttcaa tgtaaagaat cacgttcgtg tgggtgagaa tgcgatggtc acttaccggg
     5101 agaacaacgg cggttacaat ggcgaccagc aacaggaagg cggttcgatc gcgtttactt
     5161 tccgtgaaat gccgatcata cccatttatg atgtaaaggg gcatttcgga ggaggctatg
     5221 acggtccctc cggcgagccg ctggggaacg gaagcaatcc atacgcgatc atgtcacggg
     5281 agtcgaccaa ccaggctcat ttcgtaacgg tggaaggcat cgcctttgtg gaggccgatt
     5341 tcctccagca tttcacagcg aggatggcat tcggcggaag gctttggaac cagtattact
     5401 ggaatatcac ctataacccg tacgaagatt acgagtcgca tgcgaatccc aacagcgcga
     5461 cagaaaatga gcagatgagc gccagccaca actggaccaa cacagtgacc tataagaacg
     5521 attggggcaa gcacagcctg ctgatattgg gcggttatga gcaaaggggc accaacggac
     5581 gcgaatttca tgccagcgcg cagagctttt tcagcctgga cccgaatgtg gtcgaactgc
     5641 aatatggaac tccagtgaat gcccctacga gcacgatctg gcaaccgacg tctacagaat
     5701 cgttgtttgg gaggatcgat tacgcctata atgagaaata tctgatcggc gccacgatcc
     5761 gccgggacgg atattcggta ttttatcagg acaaacagta tgggaccttc ccatcggtgt
     5821 cgctgggatg gcggatatcc gaagaagact ttatgaaaga cgtcacctgg gtgaataacc
     5881 tgaagcttcg tgcaagctat ggcgtggccg gtaataatgc caatatcgcc ggaaacaatg
     5941 cctacaccag ctttagccag gggacgggtg cttcctatta cagcgtcagt ggtgctgcca
     6001 gctcgacaag ccccggattt ttccagaatc agctgggtaa ccagaatgtg acctgggaaa
     6061 gggataagat gacgaatatc ggtttggatg gaacgctttt caacaatcac tgggattttt
     6121 cgatcgagtg gtataaaaag gcgatcagcg gcctgctgtt cccggcggat cttcccgcta
     6181 cggtaggtgg tgcgactccc ccctcgatca atatcgcgga tgttcagaac acgggtctcg
     6241 acatctccac tacctatcac ggcaggatca atcatgacct aaccttcaat attacggcga
     6301 atatcacttc ctataatagc ctgatcactg cagaaccggg cagcggctat ttcgacttcg
     6361 gatattcgag ggacctggat atcgttcgta accaggtggg acatccgatc ggcgcgttct
     6421 acggattcca gaccaagggt ctttatcaat cggatgtgga tgcgggcaag ggcgctacct
     6481 attccggtgc caaagcgggg tcgttcattt acaaagatgt caatggtgac ggcgtgatcg
     6541 acgccaccaa tgaccggacc tggcttggta atccgaatcc gaaatttacg tatgggctga
     6601 acctgaacgc cgcctacaaa ggattcgatt tctcgatggt gctatatggc tcttatggga
     6661 acaaggattt caactatgta aaatactgga cagatttcta cagtaccttc cagggtggca
     6721 agaacctgga tctatacaat aaggctgcga tcgtcaataa cggcgttgtc acgaacccgg
     6781 gcgcaatatt gccgcctgcg tcttacggtc aggccatggg ttcgagcacc gtcagcagct
     6841 tctatgtcga gcccggttcc ttcctgaaat gccgcattat tcaactggga tacacgatca
     6901 cgccgtccgc tatcaagaaa gtcggtatcg acaagctgca tttctatgtg caggctacca
     6961 atcccttcat gatcacgaag tacagcggac ctgatccgga attgctgccg tctctgagca
     7021 ataatggagg atcgaataac cagaccaatg tcggcaaccg tcagaatgcc gcctttggta
     7081 tcgattacgg ggcttatccg acgaatcagc acgtattcct gttcggtatc aatgcatcct
     7141 tttaacaatt gaaaaattgc tttatatgaa aaaaattgga aaatatatac tgatctcact
     7201 gagcttgccg ctgtccgtga tattgatcta cgcctgtacc aaaaacctga agaagccccc
     7261 ggtgggctcc tacgggccca gcaacctggc cagcaaagaa ggagttgatg gtctgctgat
     7321 cggcgcttac tcgatgcttg acggtgtcgc caacggaagt acatacaacg gcggtggcta
     7381 tacgtcatgg gaaacctcga cgaccaactg ggtgtacggc ggcatcgcat ccgacgacgc
     7441 ctataagggt tcgaatacta ccgaccagcc gaatgccgcg ccgatcgaga accatagcgt
     7501 cgcatcatcg aacgaatacc tggcggagaa atactcattc tgttacaatg ccatccagcg
     7561 ggcgaatgac gtgatccggg aactgccgga tgccgctaaa gtcgatgccc aggtaacggc
     7621 ggcctttgct gcagaggaag cggccgaagc gagattcctg cgtggtgtct ttcatctgga
     7681 agccgcgaaa ctgtggcgga acatcccgta tgtgaccgaa cgtattacat atactgcaaa
     7741 caactacatt ttgcctaatc caggtccgat ctgggattct atcgaagccg atttcaccgc
     7801 tgccatggcg gtattaccca agactcagtc gcagataggc cgggccaact attatgccgc
     7861 ggaagctttt cttgccaaga cctatatgtt cgatcaccag tatacgaagg cattgccgct
     7921 gctgaccgat ctgatcacca acggaacgac ccaatccgga gcgaaatatg ctttagggcc
     7981 tttcgaagat aacttcgatg cagcgcacag gaacaattcc gagtctgtgt tctgcgtgga
     8041 gatgacggcc aatgacggtt cgggcggctg gaactcggac cagggtgata tcctgaatta
     8101 tgctgcaggt ccgtatacgg gttgctgcgg attttatcag ccttcctatt ccatggggaa
     8161 cgcgtacaaa acggacgcca atggattgcc gatgttcacg atcgaccagg ctgcgaatgg
     8221 ctggccggcc tatgatgacg tcaatttgaa aaatgaccac ggtctgttgg cgtcggatcc
     8281 ttttacgccg gcaacggaca atctcgaccc ccggatcgac tggacggtcg gtcgtcgcgg
     8341 catcccctat ctcgattggg gtctttgcgg cggagagacc tggtcgcgtg gcgatatcgt
     8401 gccttacaac cccatcaaga acgtgttctg gcattataat atgggtacca cgaccgatcc
     8461 gctggcgcag ggctgggcag ccaaccaggg cacagcgatc aattatgaca tgattcgctt
     8521 tgccgacgtg atcctgtgga gggcagagtg cgaagtcgag gggaatgacc ttgtcaatgc
     8581 tcagaaggac gtaaacatgg tgcgtaaccg catggtcgcg catccggagt actgggtgca
     8641 tacctatgta gacaatgccg acccgtccaa aggattcacc acgactcccg ctgccaatta
     8701 tgtcatcgga ttgtatggaa ccgttgccgg taccgatttt gtcacgaagg gacaggcgta
     8761 cgcccggatg gcggtgtata ccgagcgtca gctggaactg gcgatggaag gacatcgttt
     8821 cttcgatctt cagcgttggg atggtctgta tggcggaccg atgccgaaag gctggatggc
     8881 tggggtgctg aacgcctata tcaaggccga tacgaggata tcgaacccgg tgttgaacgg
     8941 tcagaagttc acggcgggaa ctaatgaatt gtatcctttg ccgcagcacc aggtcgacct
     9001 ggaagcaggc aaattgaaac aaaatcctgg ttactgatac acgtcgattt ctattttcaa
     9061 gagatggcag cttagcaggg ccatctcttt tttttaaatt tgggacatga tcaagatccg
     9121 gattttggtt gttgcggcca ttgccctgtg cgttttttcc tgcaggaaga agacgcaatt
     9181 cgagctgatc ggctctgcgc attcggggat ccatttcaat aatacgatcg tcgagaacga
     9241 ctccatcaat ccgctggacc tgaccaatat ctataatggg ggcggggttg ggatcggcga
     9301 tttcaacaat gacgggctgc ccgacattta ttttacgggt aataccgttt ccaataagtt
     9361 gtatctgaac aagggaaacc ttgtttttga ggatatcacg gatatcgccg gcgtaggcgg
     9421 caacggggaa tggtgcaggg gagcggccgt cgtcgacgtc aataacgacg gcctgccgga
     9481 tatctatgtc tgcgccaaca tcaaacagga cccgcggcaa cgaacgaatc ttttgtatgt
     9541 caatcaggga gtggatgcaa agggcatccc gcatttcaaa gaaatggggg tggaatacgg
     9601 gttggcggat acgctgtatt cgacgatggc gacctttttc gattatgaca atgatggccg
     9661 gatcgacatg tacctgaccg tgaatgggat cccaaagggt atcaatccaa ataatttcag
     9721 gaaaccggtg accgacggat cgtttccgag tacggggcat ttgtacagga acgaatggaa
     9781 tgactcattg aagcacccgg tattcaggga tgtatcgaaa caggccggta ttaccatcga
     9841 gggattcggg catgcggcta cgatcgcgga tatcaaccgc gatggctgga aagatatcta
     9901 tgtgaccaat gacttcaccg ggaacgatat tctatacatc aataaccggg acggcacgtt
     9961 taccaataag atcgcgagct attttaagca tacctccgcc aatggaatgg ggcaggatat
    10021 cgaagatgtg aataatgacg ggcttgccga tcttgtcgaa ctggatatga acccgccgga
    10081 caattaccgg aagaaaatgt tcctgaatcc aagcagttat ttgttctatc agaattccga
    10141 tcagtatggg taccagtatc aatacatccg gaatagtctc caactgaacc aggggccccg
    10201 ggtagagggt gactccgcgg gtgatcccgt gttcagcgag atcagctggc tgagcggcat
    10261 cgccgaaaca gactggagct ggacgccgat gctggcagac ttcgacaatg acgggtaccg
    10321 ggacctgatc gtcacggggg gatatcctaa agacataacc gatcacgact tccagatgtt
    10381 catgcgtcag gcttcgaata tcatctcgca tgaagacatc atgaagcaga taccggaagt
    10441 gaagatccac aacttcgctt ttcgcaataa gggcgatctg acgtttgcgg acgtgtcgga
    10501 ggattggggg atgatgacgc cgggcttttc caacggggcg gcgtatgcgg acctggatgg
    10561 ggacggcgat ctggatcttg tcgtcaacaa tatcaatgac gaggcgagga tctataagaa
    10621 catggcgcgc gaacggggcg ggtcggggga tcattttctg cgggtcgtgt tttccgggga
    10681 cagtctcaac aggggcggcc tgggtgcgtg ggtcgagctc cattatgacc atggtaaaca
    10741 gcaggtgtat gagaacaatc cttaccgcgg ctatttgtcg acgatagaga atgccgcgca
    10801 tttcgggctg gggcctgtgc ggacggtgga taccatgctg atcagatggc cggacgggaa
    10861 ggcgcagacg ttgtataacg tggcctgtga ccaggtggtc aaaaccgatt ggcgtaaggc
    10921 tcgcgatcga tatgtgcccc cgggtccacg ggtcgcgccg gcagcggttt tcagggatat
    10981 caccgactcg gcaggtatcc attaccggca gaaagagaag gactacatcg acttcaacgt
    11041 gcaaaaattg ttgccgcata aattctcgca atatgggcct ggcatagctg tcggcgacgt
    11101 ggatggcaac gggctcgatg acctggtggt gggggggtcg gcgaattata gcgcggaggt
    11161 gtttttgcag cagtcggatg gaagattcgt ggcctcgccg ttgctgaagg actcggcggc
    11221 tgctttggct aagttgagcg acgatgaagg gatattgcta ttcgacgcgg atggggatgg
    11281 ggacatggac ctttatattg cggcgggagg gtatgagcat ggtgagaaca gcgaggcgta
    11341 tacggacaga ctttatatca atgacggtca tgggcatttc gcgcttgcgc cggcggatgc
    11401 gttgccgtcg cataatgtga cgagcaaatt ctgtgtgcgg gcggcggact atgatcatga
    11461 tggaagacct gacttgtttg tgagcgggcg ggtcgatccg gggcattatc cgcagccggt
    11521 gacgagtttt atttatcaca acgagacggt cgatggtcgg gtgaggttcg tcgacgtcac
    11581 ggcgaaggtc gcgccggggt tggagaagat cggcatggtt tgcgacggca tctggacgga
    11641 catcgataat gacggatggc ccgatctggt gttggcgggg gagtggatgc cattgacggt
    11701 gtttaagaat gagcggggga tattcaggaa tgtgaccggc ggcacgggtg agagcggagt
    11761 caccggttgg tggaatagta tcgtggccgg cgatttcgac aatgacggag atatcgacta
    11821 tgtggtgggc aatttggggt tgaactcgta ttataaggcg gatgcggagc accctgtgcg
    11881 ggtatatggc aaggatttcg acaataatgg cgtgtatgac atgctgacct cgttgtacct
    11941 gccggatcag aagggcaggc tgcgggagtt tcccgcgatt gggagggacg atctgttgcg
    12001 gcagatgaat tccatgcggc accgctttcc gtcataccgg gattatgccg tggccacgat
    12061 ggacaaagtg ttgacgcccg aagaccggaa tggcgctacg atccttacgg ccaaccagat
    12121 gcgcagttgt cttttccgga atgacggagg cgggaagttt acgatgatac cgttgccggt
    12181 ggaggcgcag ttttcggtga tcaatgggat ggtggcgggt gattttgacg gggatgggaa
    12241 tctggatatc gtcatgagtg ggaatgatta tgggacggag gtttctacgg gaagatatga
    12301 tgccatgaat gggttgttgt tgcggggcga tggttcttgt ggtttcttcc ccgcaacgat
    12361 attgcagtct ggcattttta ttccgggtga cgggaaggcg ctggtggcat tgcggggagc
    12421 cggggggcat tatttattgg cggcggggca gaacaggggc ccattgaagg tatttaagcg
    12481 tcgcatggat gggcgccagg tggcgttgat gccgtcggag gttgcggcga tggtgcgatt
    12541 gaaggatgga agggtgagga gggaggagta ttattatggg gagtcgttct tgtcgcaatc
    12601 ggggaggttt atggattgga atgggccggt ggttgaggcg gagatcgtcg atgagaaagg
    12661 gaggaagagg gtggtgagat gatggagaac atggcaggga gcgcggcggc ggcagtgagg
    12721 aaggagcgcg gcgggcggtg aggatggagc gcggtgggcg gtgtaagttt tttatctttg
    12781 ggatatgagc aattttccgt tatggctggt gtatgcgttg ttgtccgcca tatttgccgc
    12841 cttgaccgcg atattcgcca agatcggttt aaaggacgtg aatgccgacc tggctacggc
    12901 catacggacg gcggtgatcc tgctgatcac ctgggggatc gtgttatttc ggggtaacgt
    12961 ggcgggtttg ccggggctga cgcggaataa ctggattttc ctggtcttat cggcgattgc
    13021 gacggggatg tcctggcttt tttattaccg ggcattacag atcgggcagg tgcagcaggt
    13081 agcggtcatc gacaagggta gtatattctt tactatattt ctttcttttt tctttctcaa
    13141 agaaccgttg actccaaaag tgctgctggc tgctggtctg atctttgccg gaatgctcgt
    13201 tctggtctgg aaataagctg agagggcgga aatctccccg gggcccttaa caaacgggaa
    13261 tatcttatta cattagacag accggaggcc tgtcggccag aatttagcca ttaggcgaag
    13321 attccgaacg agtcctcggc tgtggcgagg acgaagtctc cagatattaa ataaaagccg
    13381 tgtcgttgat cgggatacat aatgaaaaag agctgctcca gcaggtgtcc cagggcgaca
    13441 gggatgcgtt caggcgtttg tatacggggt atttcgggca tgtgcagcga tatatctatc
    13501 tgttcgagcc ttcgcgggac aatctcgatg agcttactca ggatgtattc gtcagagtat
    13561 gggagaagag gggtcatttg ggtgaagtgg aatcctttaa agattatctc ttttttattg
    13621 ccaggaatgt cgtgttcaat tatttgcgcg gtctgaaggt acagaagaag atcagggagc
    13681 tggaggggtt ggatggcacg ggcgtttacg agacggagga gctggtattg ttcaggcaat
    13741 attaccggat agcgttggaa gcgatggaaa agctgccacc gggcaggcgt aaggtattaa
    13801 aaatgagcat tgatgatggg ttgagtctgg atgagatagc ggcggagctg aagatcagcc
    13861 gggcgggggt gaagaagcag ttgtatgcgg ccacggcttt tgtcaggaag tacttgcagg
    13921 agcatgggga gattacggca ttgctttttg tgtttctttc gctttttgag cgatagggtg
    13981 tgtgaagttt tgatggtttt atcggaaaat ttccgatgat tcatgtaata tttcacattt
    14041 ggctgttatt ttttgcgtga aatatcatcg taccacagga aagaatctcg caacgtccgg
    14101 aaataaatta agcttgaagt tgaaaccctg cctcccccgg tttcagtacc caggaaaaat
    14161 tgcttttaaa gcctactgga agaaaagata aataccgtgt cgtaatctta ttaattttca
    14221 actgtttgta gagttttaag tttttctcaa aaaaatttat gggtcgaggt atcccgggga
    14281 cttcccgaag tgtctttatt atatatcacc ttgttttatg aatgttaagg aggctcagca
    14341 atttgtcgcg gattatgcaa atggggaatt caccccgacg gagcatgcgg catttttgca
    14401 gtgggtaagg ggagcgggcg ttagagagtt ggagctcatc gctgaagaac atgagactca
    14461 tcatggacag tgggtatttt ccacagaagg accttcgccc gagtgggtag gagcattgga
    14521 agatcggttg gatcggttgg cggagaaacg tgtgaaggcg ccggtggtcc gtatgccttt
    14581 tggaaagagg gtatggatag tggcggcgtc cgtagccatc ctcattatcg cagggacttt
    14641 ttggtacagc caaactggcg gatcattgaa taaagaagag cgtcagcagg cattggcaac
    14701 actgacgcag acgatgaagg tagagagggg tggtgagcag cgttccgtta cactgccgga
    14761 tggcagcaag gtttggctga atgtagcctc tatcctgaaa tatccggtga agttcagcgg
    14821 gccggatcgg gtggtggaat tgtccggaga aggttatttt gaagtttcgc aacgttccga
    14881 tatgccattt cgggtcctca tcaaagatgc ggaggttaag gtcctgggca cacactttaa
    14941 tatcatggct tacgatgatg agccggtcgc ccggacgtct ctgatcgacg gttctgtaag
    15001 cgtcgaaagc gggtcacaac gagttacgct ccatcctgga gagcagggag aaataccgta
    15061 ctcatccccg ggagcaactg ggcctatcaa ggtcatcccg gggatcgatc ccagcgcggt
    15121 tgtagcctgg agggacgggg tcctgaagtt cgagggcacc cctatgcatg agctgttgcg
    15181 gaccctggga agatgttatg acgttgaaat acagatcgat cagaatgtac ccgatacacc
    15241 tggctacgga attttttccc gaagccaggg tcttgactcg ataatgaaac aattgaaacg
    15301 agaccatcta cagtttataa ccaatggaaa aacagtgaga gtcacaatgg caaaataatt
    15361 gtccattacc attgaaccaa gtccgccagt tacagcccgc ggtcatgagt aaatagaaaa
    15421 ataagtaatt attttatgtt tattcctgat ttgtaggctg taactttatt tattaaacca
    15481 cccggatcaa tgaggtccaa ccgactggct gtattcctat taattgcctg cttgttgaat
    15541 tgccggccat cctattctca aacggatcct ctcatcaccc tgagcgagaa ggatgttcca
    15601 ttacagaagg tcctggatga aatccacaca aagacgggtt atgcatattt cggcgaaggc
    15661 gactggccaa agatcgcgca caaggtgagc atatcggtca ggaatgcttc gatccgcaag
    15721 gtattggata cctgtttttt cgatcagccg gtgctctatg aattggatga aaaggagcga
    15781 tttatctttg tgcgtttgcg accgaaggaa gagcggaagg tacatggttg gatcgtcgat
    15841 gagaacaggg accccgtcgg cggggcatcc atcgtcgcac ggggcgacgt ttcgggggtg
    15901 agcaatgatg acggtgaatt cgaactttcc acccatttct cggattctaa gctgtcggtc
    15961 agcagcatcg gctatgagcc tcaggaactg ccgttgccac cgtcaggaaa ggatattacc
    16021 atcaccctga gatcgcggac gaaggcgttg atcgaagcag tcgttcttca tactggttac
    16081 caggatgtga aacggaaagc atctacgggt tcgttcgacg aagtagataa cgacctgctg
    16141 aatcgtcgcg tgtcgaccaa tgtgctcgac aggatagacg gggtgacgag cggtgtactt
    16201 ttcaacaaga atatcgtgac gagcgccaat cagtcggcta ttaccattcg cgggcgcagc
    16261 acgatctatg cgaaccccaa cccgctgatc gtcgtggata atttccccta tccgggggac
    16321 atcaataata tcaatccagc ggatgtggag agtatcacgg tgttgaagga tgccgcggcc
    16381 gcgg
//
LOCUS       DASYSV010000291         3535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1852316, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000291 DASYSV010000000
VERSION     DASYSV010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852316"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..332)
                     /locus_tag="VGQ51_08775"
     CDS             complement(27..332)
                     /locus_tag="VGQ51_08775"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036701.1"
                     /translation="MKFRESPYINRRKKTIEFYVHHVNATSAAHISLAGSFNHWAPDE
                     LQMAPAKDGFWKISIPMLPKGKYYYKFLVDAKMWMEDIENRLREPDGVAGWNSVLTV"
     gene            555..1247
                     /locus_tag="VGQ51_08780"
     CDS             555..1247
                     /locus_tag="VGQ51_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9036702.1"
                     /translation="MAETKILLVEDEKKIAESLRKGLSEQHYEVDVAYDGIAGRKLFD
                     SNSYDLAILDINLPLLNGYELARHIRSYNEDILVIMLTAMNTTEDKIEGFEAGADDYI
                     VKPFDFQELVVRIRALLKRLHHQPGAGTNILRVGDLIMNLDSKEVSREGRNIPLTAKE
                     FQLLEYFIRNKNKVVSRIDIALNVWDIDFDTKTNVIDVYVNFLRKKIEKDFSSKLIYT
                     QVGMGYVLKETP"
     gene            1298..2662
                     /locus_tag="VGQ51_08785"
     CDS             1298..2662
                     /locus_tag="VGQ51_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV9036703.1"
                     /translation="MLLFTLLVTAIIVLLTGSVYYFAKLERKQVYNKRLRSRASYNMQ
                     LYSLMGANADGLLSRMDSASILGTISSRSIGIYTDYGKVLYSFNMPGAPPLRVSQSLL
                     QDAKTKGEVYFTVGKLEAVALHREAANRDFIVVVAGHDDDGLERVDTLNKILVISLVL
                     SICLTALVSFLFARQLLRPIAQIIREVKEISSFDLSHRIRAGTGQDEMSQLANTFNDL
                     LGRLQESFAIQRRFISNASHELSTPLTSVSSQVEVVLQKERSADEYKQVLASVREDVQ
                     QMRQLTKSLLEIAKTGSQGGIELGEVRVDEVLMKVVADVKKLNTEYSVELDFGEFPED
                     EKDFVVFGNSELLFIAIKNLVENGCKYSMDKRSAVDLSFAWHKVYIRVVNRGNVIGPD
                     EINQIFQPFYRGQDTANVRGFGLGLAMAQRIIALHKGTIHVASDEANGTHFTIELPAI
                     KIFN"
     gene            2703..>3535
                     /locus_tag="VGQ51_08790"
     CDS             2703..>3535
                     /locus_tag="VGQ51_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEV9036704.1"
                     /translation="MPWIQPTNPVHNLAASGLVAVLPILVIFWALIIRKRKGWQAGLL
                     AIAAAAIIAIAVYHMPVHLALLSMINGAAYGLFPICWIVLTAVFLFNIAVRSGQFGII
                     RNFMAGISADRRIQALLIAFSFGSFLEGTAGFGAPVAITAAMLTGLGFDPLYAAGLCL
                     IANTAPVAFGSIGIPVTVMSQVTSLPEQAISEIVGRTLPFLSALLPFYLVVLMAGFKK
                     AKEVAPAVLVSGLSFAIIQFLTSNYLGPALPDVLAGIGSIISLLVLLRFWQPRSIWRF
                     AG"
BASE COUNT          806 a          976 c          902 g          851 t
ORIGIN      
        1 cgaaggaggc cgcttatctg tgttttctaa acggttaaaa cgctgttcca ccccgccacg
       61 ccatccggct cacgaagccg gttctcgatg tcttccatcc acatcttggc atcgaccagg
      121 aacttgtaat aatacttgcc cttgggcagc atagggattg agatcttcca aaaaccatct
      181 tttgccggag ccatttgcaa ttcgtccggt gcccaatggt tgaaggaacc tgccaggcta
      241 atgtgggctg cggatgtcgc attcacatgg tgaacgtaaa actcgatcgt ttttttacgc
      301 cggttgatat acggcgattc gcgaaatttc atagcaaccc ccttttttag attttaaaaa
      361 cgctttcttt aggatatact caaatagcca gcaaacggga attctacgag taaatgaatt
      421 tgaagagtgt acaaaattta cacttttttt tggcatattg caatacgacc agacattttt
      481 ttgctaaatc ggtttaattt gtttttaatc ggttgcgggg ggcgggttcc ccggggattg
      541 gatatattta tattatggca gagacaaaga ttttattggt tgaagatgag aagaaaatcg
      601 ccgaatccct gcgaaagggg ttatccgagc agcattatga ggtcgatgtg gcctatgacg
      661 gcatcgcggg acgaaagctc tttgactcca actcatacga tctggccatt ctcgatatca
      721 acctgcccct cctcaacggc tatgagctgg cccgtcatat ccgcagctat aatgaagaca
      781 ttctggtgat catgctcacc gccatgaata ccactgagga taagatcgag gggttcgagg
      841 ccggggccga tgactatatc gtcaaaccct tcgatttcca ggagctggtg gtccggatcc
      901 gcgcccttct caaacgcctg caccatcagc caggcgccgg gaccaatatc ctccgggtcg
      961 gcgatctgat catgaacctc gacagcaagg aagtctcccg cgagggcagg aatatcccat
     1021 tgaccgcgaa ggaattccag ctcctcgaat actttatccg caataagaac aaggtggtca
     1081 gcaggataga tatcgcgctc aacgtctggg acatcgattt cgataccaag accaatgtca
     1141 tcgatgtcta cgtcaacttt ctgcgaaaga agatagaaaa ggacttttct tccaagctca
     1201 tttacacaca ggtgggaatg ggctatgtcc tgaaagaaac cccctgacgg gcgggacgcc
     1261 tgtccgcgaa ctatttgtga agatccgcta taaaataatg ctccttttta ccctgcttgt
     1321 cacggcgatc atcgtcctgc tgacgggttc ggtgtattat tttgccaagc tcgaacggaa
     1381 acaggtgtac aacaaaagac tccggtccag ggcttcctac aacatgcagc tttattctct
     1441 gatgggcgcc aatgccgacg gcctgctcag tcggatggac tccgcctcca ttttgggaac
     1501 gatatcatcc cgaagcatcg gcatctatac cgattacgga aaggtcctct atagcttcaa
     1561 catgcccggt gcgccgccat tgagggtctc ccagtccctc ctgcaggacg caaagacgaa
     1621 aggagaagtc tatttcaccg tcggtaagct ggaagccgta gcgcttcacc gcgaggccgc
     1681 gaacagggat tttatcgtcg tcgtggccgg tcatgacgac gacgggctcg aaagggtaga
     1741 cacactgaac aagatcctcg tgatcagcct ggtcctcagc atctgtctca cggctttggt
     1801 gagcttcctt ttcgcccgtc agctgcttcg gcccatcgcg cagatcatcc gtgaggtaaa
     1861 ggaaatctct tccttcgacc tctcgcatcg tatccgtgca ggtacgggcc aggacgagat
     1921 gagccagctg gccaacacct tcaatgacct gctgggccgg ctccaggaat ccttcgccat
     1981 ccagcgccgc ttcatctcca acgcctcgca cgagctttcg accccgctta cctccgtcag
     2041 tagccaggtc gaggtcgtat tgcaaaaaga acgctccgcg gatgaataca aacaggtgct
     2101 ggcctccgtc cgcgaagacg tacagcagat gcggcagctg accaaaagcc tgctggaaat
     2161 agccaagacc ggctcacagg gtggcatcga gctcggagaa gtacgcgtgg acgaggtgct
     2221 gatgaaggtc gtcgccgatg taaaaaagct gaataccgaa tacagcgtcg agctcgactt
     2281 cggtgagttc cccgaagatg aaaaggattt tgtcgttttc ggcaatagcg agctgctctt
     2341 catcgcgatc aagaacctgg tcgagaacgg ctgcaaatac tctatggata agcgatccgc
     2401 agtcgatctc agctttgcct ggcacaaggt ctatatccgg gtggtcaatc gcgggaatgt
     2461 gatcggccca gacgaaataa accagatctt tcaacctttt taccggggac aggatacggc
     2521 taatgtccgc ggtttcggtc tgggcctggc catggcccaa cggatcatcg cactgcacaa
     2581 agggaccatc catgtcgcct ccgatgaggc caacggaaca cacttcacca tcgagcttcc
     2641 cgccatcaag atatttaact aggggaaggg caccagctcc ggatttttgc cgatattcat
     2701 ccatgccctg gatacaacct accaaccctg tgcataatct tgcggcttcc ggcctggtgg
     2761 ctgttcttcc cattttggtg atcttctggg cgctgatcat ccggaagaga aaaggatggc
     2821 aggcagggtt gctggctatc gcagcggctg cgatcatcgc catcgccgtc taccatatgc
     2881 ctgttcacct cgccctactg tccatgatca atggggccgc atatggactt tttcccatct
     2941 gctggatcgt cctgaccgcg gtatttctat tcaacatcgc cgttcgcagc ggtcagttcg
     3001 ggatcatccg caattttatg gccggcatca gcgccgaccg aaggatacaa gctctgctga
     3061 tcgctttctc cttcggttcc tttctcgaag ggacggcagg tttcggcgcc cccgtggcga
     3121 tcacggccgc gatgctgaca ggattgggtt tcgatccgct gtatgccgca ggtctgtgcc
     3181 tcatcgccaa cactgctccg gtcgccttcg gctccatcgg aatacccgtt acggtcatgt
     3241 cgcaggtgac ttcattgccg gaacaggcta tttcggagat cgtcggacgc acactgccct
     3301 tcctctcagc attactgcct ttttacctgg tggtactgat ggccggtttc aaaaaagcaa
     3361 aagaggtcgc gccagcggta ctggtctcgg gtctctcttt cgccatcatc caattcctca
     3421 cctccaatta cctgggaccc gccctgccgg acgtactggc agggatcggc tccatcatca
     3481 gcctgctggt tttgctgaga ttctggcagc cacggtcgat ctggaggttt gcggg
//
LOCUS       DASYSV010000292         9873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1079006, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000292 DASYSV010000000
VERSION     DASYSV010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079006"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VGQ51_08795"
     CDS             complement(<1..911)
                     /locus_tag="VGQ51_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036705.1"
                     /translation="MFRLSLKTAFRNLLKRRAFGSLNIAGLSIGITCAAFILLWVEDE
                     MAFDHNFALHDHLYRIIENEKSDQGVNTMMDIPGEVAGAVKTEIPGIKNSARVSFHLD
                     ELAVIGDKKIRTNGLYADTSFLSMFTINLLRGDRVTALGQPQSVVISQSLARACFGDE
                     DPLGKTIRMNAGESYSVDGLYKVTGVFADLPANCSFRFQWLSPYTTWENFLPWVKGWS
                     NPMAETYVQLEPSASPAAVQGQLTHYLAQKVSGQKNTLYLWSMNDWHLRGKIENGVPT
                     DSVIRYVRLFSLIAIAILLIACINFMNL"
     gene            complement(968..2368)
                     /locus_tag="VGQ51_08800"
     CDS             complement(968..2368)
                     /locus_tag="VGQ51_08800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV9036706.1"
                     /translation="MRHIRKFFILLILLAGAGSPVFAQSKPSFGELKEYEGLYQFANP
                     PTLKMAASPVDTLLYAIINQARYPLRPKGKDVFVNSTGDPVTFLRDAKGEIDRYIAGK
                     DTFRLLDKAVSFPTQMWYPRMVADPKHYTYRYSPPSDNGDGLSTGSLEGTGLDPSLLS
                     NMVDSIVAGVYPNVHGILIIKDRRLVFEEYFYEYTRDSLQEMRSATKSVISALTGIAI
                     RKGLISGVGQRLAALFPEYRWENPSPLKDRITVRDLLDNQSGVNYDEAWDKSIGNENA
                     MDYSSDWVKYTFDLPMLDTPGVHGRYNSGNPITLGRIIEKASGQPLAQFASANLFRPL
                     GITRFKWNFKPDRSNAENFCQLYLTPREMAKFGLLFLQDGVWQGHEVIPAAWVKESTS
                     KHSVVQNVNYGYLWWLKYLDAGATRYYSFAAQGNGGQKIYVFRSLNLVVVTTGGNFNT
                     QSPADVLIRKYILPGL"
     gene            2434..2940
                     /locus_tag="VGQ51_08805"
     CDS             2434..2940
                     /locus_tag="VGQ51_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036707.1"
                     /translation="MKAPIFSFLTSVLFLLLSHPPAGAQRPAKRDTFLVDPSKPVLPP
                     PRTNPETRAHIKKEAVAEYREKSKHGEFVVKLFQTSKTMSYRIDVDFEGLPGSDTVHL
                     PDLGNEPRPALQKGDSTTSCLVGFLDNDNKFREVKLIFVTPKGNQFKITTVRHWHVST
                     HYRLVANQ"
     gene            3052..4830
                     /locus_tag="VGQ51_08810"
     CDS             3052..4830
                     /locus_tag="VGQ51_08810"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HEV9036708.1"
                     /translation="MRTIASSPLFWTAFLVAGVVISSDLVVSCEHDHARRALDTVAVN
                     PAFATDPPIADDLTIQLLDKLSDDGNALLKVTFPKGRIGSPYLAFMAGNKKTVLRDDG
                     LGPDEVKGDGVYTGVVQADLDALREMRRRQIQQNIKEPIPRTVFRGRIVAGTTRIMPT
                     DLKALFEGGLIHVHWPPFPMSKSLTDSIQANSVFVTDPSVIGDTSRTYDPCTNKGHKM
                     GPWTFGFLMTQLANNSATGVSPSRFVLNWLLSYTHTQTVNSDALDARAGMAAFIHTWL
                     VNSNGLLDSTLDLSIAPFRLMAIVNRLDLRSNPGYLGSGGNAGEGRFVFTGLDAGCTA
                     QAFTVIFEYGINKTTCPAIKAYAQHWYDLKDFAIGSPAYNNALQAITDQFTLAGTNSA
                     KPNGSSLDQLRTNETQFGSNWELREFNIDPASHLLFNTTVKQTPQARFNQTTILNTFM
                     TQHAAAIKKNTYTVPLSFEFNGISDTPFLAGMAPVPPGFWAQPGAAATANDTVREMLS
                     LQTCDGCHNIETGTLFRHVSPPLTAGSPALLSGFLVGTTVTDPAEIPGTWKFADLQRR
                     AQDLVDLVNSPCVFIPFHFAPLNMTH"
     gene            complement(5137..5577)
                     /locus_tag="VGQ51_08815"
     CDS             complement(5137..5577)
                     /locus_tag="VGQ51_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036709.1"
                     /translation="MPPAERHQPKRSTYVTESRRSTGKDHPNESERLLDDAKTPIREI
                     DAVLHLERMGRDGGHRSADRELPDEPPRSAIRELPDGCFVSYGGNPFLVADGRLFPWT
                     PFGYGENATVPEGDKVEVLTPASIVRAFRAGYAPQIALSPAGNA"
     gene            5674..6480
                     /locus_tag="VGQ51_08820"
     CDS             5674..6480
                     /locus_tag="VGQ51_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036710.1"
                     /translation="MEHLIKAGRWFFALCLAGVAGQQFYYGDFRPCFVPAWPSPMPGQ
                     IVLVYLFSIALIWAAIALVSGKLARITLLLLGWLFLALVLLCHIPFHLVHDPEAGNIG
                     VWNNAFKELCFSGSALIMASSFPEDTSPRLAAIDRPLENLVPIGTFFFSIMLIVFGME
                     HMLYGEYVKALVPDWIPGHLFWTYFAGVALIGAGVGIMLRFKLRLIALLTGGMIFIWF
                     LVLHIPRAVVAPVSDNGNELTSVFQSLGFSGVAFVIACVWPARSRLTALA"
     gene            complement(6519..7349)
                     /locus_tag="VGQ51_08825"
     CDS             complement(6519..7349)
                     /locus_tag="VGQ51_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV9036711.1"
                     /translation="MSKKFLLLILLACYCGSARSQVVYGDNAAAGHYLSTRGIRLYYE
                     TYGQGAPLLMLHINGGSINVFTNQIPYFAKNYRVIAVDTRAHGKSVDAGDSLTFEMMA
                     DDFNALLDSLHLDSCFVLGWSDGGISGLLLALRHPEKVKKLVVSGPNLWPDTTGLEPF
                     IYHWLERMADSMRRLPPNPRTRNDLKIVELDLKEPHMTLEQLHAIRCPTLVMGGDHDA
                     IPSYHLWQIFRNIPKSYLWLVPGSGHFVAVYKANYFNAVVGDFFRNPYVRIEGMGMLH
                     "
     gene            7490..8584
                     /locus_tag="VGQ51_08830"
     CDS             7490..8584
                     /locus_tag="VGQ51_08830"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEV9036712.1"
                     /translation="MPKLTVMRHTAILSLAVASLTPLASRSQITTPRVPSPAAAVSQT
                     IGISTVTVNYSRPSVKGRQVWGTLVPYGWNKQGFGLGNDAPWRAGANENTVLHLSHDA
                     KVEGHTVPAGDYGLFFVINKDNTGVVVLSKDYRSWGSFFYDPQHDEMRADISLRAIPE
                     TELLTYDFQNVTKNSAELVCNWEKMQFPVKIEFAVDDIVMANAAQELKGTVGFNWQGF
                     SSAANYALQNKVNYDQALTWIDQAIAINPNFTDLRIKSALLKAKGKDADADNIMKTAL
                     DASTEVELNQYGYQLLAQNQQDKAIDAFVLNTRRHPTSANVFDSLGEAYALKGDKKNA
                     IVNFKKSLTMDPPAATKANSEKYLKQLGAM"
     gene            complement(8650..9060)
                     /gene="tsaA"
                     /locus_tag="VGQ51_08835"
     CDS             complement(8650..9060)
                     /gene="tsaA"
                     /locus_tag="VGQ51_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEV9036713.1"
                     /translation="MNDFVLEPIGSIKSPLKNLDEAPKQGYESAPEAWIELRKEYAPA
                     MDGIRPGDAIILITWLHRASRNVLKVHPRGEERNPLTGVFATRSPDRPNPLGLHRVNV
                     LAIDGVRIKVGPLEAIDGTPVVDIKPVLSQSQDA"
     gene            9291..9728
                     /locus_tag="VGQ51_08840"
     CDS             9291..9728
                     /locus_tag="VGQ51_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036714.1"
                     /translation="MLHAKDLRFGNKVLNRRGEVITVQQIHSNSIVYDTAISVSRRHL
                     SKVSGYDATYTAEVLEVVKEAGLREIEPIVLTPKVLEKCGFRNFIREEWIVTYGNKHT
                     DFEFTEDGLRLKHPSPSRVGIKYLHQLQNFFYAIAGEELQIEL"
BASE COUNT         2212 a         2774 c         2655 g         2232 t
ORIGIN      
        1 aggttcataa aattaatgca ggcgataagg aggatcgcga tggcgatcag cgagaacaga
       61 cgcacatacc ggataacact gtccgttggc acgccgttct cgatctttcc gcggagatgc
      121 cagtcgttca tactccacag gtaaagagtg ttcttctggc cactgacctt ttgggcgagg
      181 taatgcgtca gttgtccctg gacggcggcc ggggaagcag aaggctcgag ctgtacatat
      241 gtttctgcca tgggattgga ccagcctttt acccaaggga ggaaattttc ccaggtggtg
      301 taaggggaca accactgaaa acgaaagctg cagttggctg gcaggtccgc aaagacaccg
      361 gtgactttat agaggccatc cacggaatag gactccccgg cgttcatcct gatcgttttg
      421 ccgagagggt cctcatcacc gaaacaggcc cgtgccagag actggctgat gaccaccgac
      481 tgcggttgcc cgagtgccgt gacgcgatcg ccgcgaagga ggttgatcgt gaacatggac
      541 aggaaggacg tatccgcgta gaggccgttg gtcctgatct ttttgtcacc gatcaccgcg
      601 agctcgtcca ggtgaaagct gacccgcgca ctgttcttta tcccgggtat ttcggttttg
      661 accgctccgg ccacttctcc cggaatatcc atcatggtgt taacgccctg gtcggatttt
      721 tcattttcta tgatgcggta tagatggtca tgcaaagcaa aattgtgatc gaaggccatc
      781 tcatcctcca cccagagaag gatgaaggcg gcgcaggtaa tgccgatgga aagaccggcg
      841 atattgaggg aaccgaaagc gcgacgcttc aggagattgc ggaaagctgt tttcagcgaa
      901 aggcggaaca tatggcggaa cggtggtttt ataccttaaa agatagtact tcgaaaatcc
      961 cgaaaggtta caggccaggc aggatatact tcctgatcag cacatccgcc ggtgactgcg
     1021 tgttgaaatt gccgccggta gtcactacga caagattcaa tgagcggaaa acatagatct
     1081 tctgcccgcc attaccctgg gcggcaaagc tgtaataccg cgtcgccccc gcgtcgagat
     1141 atttcagcca ccacagatac ccgtaattga cgttctgtac gacggaatgt ttgctcgtcg
     1201 actccttcac ccaggcggca gggatcactt catgaccttg ccagacgccg tcctgtagaa
     1261 acagcagtcc gaatttcgcc atttcgcggg gtgtaagata cagctggcag aagttttccg
     1321 cattggagcg atcgggtttg aaattccact taaagcgcgt gatcccaagc ggacgaaaca
     1381 ggttggcgga ggcgaattgc gccaacggct gtccggaggc tttttcgatt atccgcccga
     1441 gggtgatcgg attgccggaa ttataacgtc cgtggactcc gggagtatcc agcatcggaa
     1501 gatcgaaggt gtatttcacc cagtcgctcg aatagtccat ggcgttttcg ttaccgatgg
     1561 atttgtccca ggcttcgtcg taattgacgc cggactggtt atccagcaag tcgcggacgg
     1621 tgatccggtc tttcagcggc gacggatttt cccatcggta ttcaggaaac agcgccgcca
     1681 gccgttgacc cacgcctgag atcagtcctt tgcggatggc tatccccgtc agtgccgaga
     1741 tgacactctt ggtagcggat cgcatttcct ggagactgtc gcgggtatac tcatagaagt
     1801 attcctcgaa gaccagccga cgatccttga tgatgaggat gccatgtacg ttgggataga
     1861 cgcccgcgac aatgctgtcg accatgttcg acaataagga cgggtcgaga cccgttcctt
     1921 ccaggctacc cgtacttaac ccgtcgccgt tatccgaagg cggggaataa cgataggtat
     1981 agtgtttcgg atcggcgacc atccggggat accacatctg cgtgggaaag gagacggcct
     2041 tatccagcag acggaaagtg tcttttccag cgatataacg atcgatctcg cccttcgcgt
     2101 cgcgcaggaa ggtcaccggg tcgccggtcg aattcacgaa tacgtctttc ccctttggac
     2161 gcaacggata acgggcctgg ttgatgatcg cataaagcag cgtgtcgact ggcgaggcgg
     2221 ccatctttaa cgtcggtgga ttcgcgaatt gatagagccc ttcatattct ttcaattccc
     2281 cgaaagacgg tttggattgg gcgaaaacgg gactgcctgc gcccgccagc aggataagaa
     2341 gaatgaaaaa ttttcgaata tgccgcatac ggtaaattac gcatttcccc gacacttgtg
     2401 ccaccgaatg aaatacccta tctttcgccc gatatgaaag caccgatctt ttccttttta
     2461 accagcgtcc tgttcctgct attatctcat cctcccgccg gcgctcaacg tccggcaaaa
     2521 agggacactt tcctcgtcga cccctccaaa cccgttcttc cgccgccccg gaccaatccc
     2581 gagacccgcg cgcatataaa aaaggaagcg gtcgcggaat accgcgaaaa atctaagcat
     2641 ggagaatttg tcgtcaagct gttccagaca tccaaaacga tgtcctaccg gatcgatgtg
     2701 gattttgaag ggcttcccgg ctcggacacg gtccaccttc ccgatctggg taacgagccc
     2761 cggccggcgc tgcaaaaagg ggactcaaca acctcctgtc tcgtcggttt cctggataat
     2821 gacaataagt tccgcgaggt gaagctgata ttcgtcacgc cgaagggaaa tcaattcaaa
     2881 atcacgacgg tcagacactg gcatgtgtcg acccattacc ggttagtggc aaatcaatag
     2941 gcgcgccgtt gccaaaaaat accataaagt gggaataccc ggacgccccg cggcggttta
     3001 cttttgtgac gatcgttcct tcagggcgac aaaagttaaa ttccatcagc cgtgcgtact
     3061 attgccagct ctccgctatt ctggacggcg ttcctcgtcg caggcgtcgt catatcttcg
     3121 gatctcgtcg tctcctgtga acacgatcat gcccgaagag ccctcgatac ggtggccgtc
     3181 aatccggctt tcgccaccga cccgccgatc gccgacgatc tgacgataca gctgctggac
     3241 aagctttcgg acgatggcaa tgccctgctg aaggtcacct ttccgaaagg aaggatcggc
     3301 agcccctacc tggcctttat ggccgggaat aagaaaacgg tcctgagaga tgacggcctc
     3361 ggtcccgacg aggtgaaagg ggacggagta tataccggtg tcgtacaggc ggacctcgac
     3421 gccttgcggg aaatgagacg ccggcagata cagcagaata taaaggagcc catcccgagg
     3481 accgtctttc gcggacgcat cgtcgcagga acgacccgca ttatgcccac cgatctgaaa
     3541 gcccttttcg aagggggttt gatccatgtc cactggccgc ctttccctat gtcgaagagt
     3601 cttaccgatt ccattcaggc caattccgtg ttcgtgaccg acccttcggt gatcggggat
     3661 acttcacgga cctatgatcc ctgtaccaac aaaggacaca agatggggcc atggacattc
     3721 ggattcctga tgacccaact ggccaacaat tccgcaacgg gtgtcagtcc atcgcgtttt
     3781 gtgctcaact ggctgctctc ctatacgcat acgcagacag tcaattcgga cgcgctggat
     3841 gcgagggcgg gcatggcggc attcatccac acctggctgg tgaacagcaa tgggctcctg
     3901 gacagtacgc tggacctgtc catcgctcct ttccggctaa tggcgatcgt caaccgtctc
     3961 gatctgcgca gcaacccggg atacctcggt tccggcggca atgctggtga aggaaggttc
     4021 gtattcaccg gtctggacgc gggctgcacc gcacaggcgt tcaccgtcat cttcgaatac
     4081 ggtatcaaca agaccacctg tcctgccatc aaggcctatg cgcaacattg gtacgatctg
     4141 aaggattttg ccatcggatc gccggcctat aacaatgcat tacaggcgat caccgaccag
     4201 ttcacgctcg caggtaccaa ctccgccaaa cccaacggaa gctcgctcga tcagctaagg
     4261 accaacgaga cgcagttcgg ttccaactgg gagcttcgcg agttcaacat agatcccgcc
     4321 agtcaccttc ttttcaatac taccgtaaaa caaaccccgc aggcgagatt caaccagacg
     4381 accattctca ataccttcat gacgcaacac gccgctgcta ttaagaagaa tacctatacc
     4441 gtccccctgt cgttcgaatt caatggcatc tccgatacgc ctttcctggc gggtatggcg
     4501 ccggtgcctc ccggtttctg ggcccagccg ggcgccgcgg cgaccgccaa tgacacggtc
     4561 cgcgaaatgt tgtcgctgca aacctgtgac gggtgtcaca atatcgagac gggaacgctt
     4621 ttccggcatg tcagcccgcc gctgaccgcc ggcagccccg cgctcctttc gggtttcctc
     4681 gtcggcacga ccgtcacgga tcccgccgag atcccgggca cctggaaatt tgcggatctc
     4741 cagcgaaggg cgcaggacct ggttgacctg gtcaatagcc catgtgtttt cattccgttt
     4801 catttcgcgc ccttgaatat gacgcattga gccgcgtcat gcaggaccct gcccccaatt
     4861 ttccctaaac ttccccttcg ggtgaacgcg ggctgtcaaa accaacaccg acagcagcgt
     4921 catcgagttt catatcagaa ataccaccta taacggaaat tattgcatta caccggcgac
     4981 acttcccgcc gacttacagg tttacaagga ttcactcaga ttacccctct tgcaaacttc
     5041 ttcttccgga ggtggcggtt tcggcaatcg cggagggatc atccatcaca tcgattgcga
     5101 atatgggaac atggtgaagc gaacacactt agcgcatcac gcgtttcccg ccgggctcaa
     5161 cgcgatttga ggcgcatacc ccgcgcgaaa ggcgcggacg atcgacgccg gagtcaggac
     5221 ctccacctta tcgccttccg gtaccgtcgc gttttcgcca tatccgaagg gcgtccaggg
     5281 aaatagccgt ccatctgcca cgagaaaggg gttgcctcca taactgacga agcagccgtc
     5341 gggtagctcg cgtatcgctg atcggggcgg ctcgtcgggc aattcgcggt ccgccgatct
     5401 atggcctccg tcgcgcccca tcctttcgag gtgaaggacc gcgtcgatct cccggatggg
     5461 cgtcttcgca tcatccagga gcctctcgct ttcgttcgga tgatccttcc ccgtggatcg
     5521 acgcgattct gtaacatagg ttgatctttt cggctgatga cgttccgcag gcggcacagc
     5581 aatacgaatg taacatcttg taccgacttg ttccaagttg aaccgcggcg tcagggggta
     5641 ttcgcataaa ttacgtgaat cattttttgc aacatggaac atctgatcaa agccggccgc
     5701 tggttctttg cgctctgtct ggcaggagtg gcaggacagc agttttatta tggggacttt
     5761 cggccttgtt tcgtccctgc atggccgtcc cccatgcccg gccagatcgt gctggtctat
     5821 cttttcagta tcgcgctgat ctgggctgcc attgcgctcg tctccgggaa gctggctcgt
     5881 atcacgctat tgctgttagg ctggcttttt ctggcgctcg tccttttatg ccatatccct
     5941 tttcacctgg tgcatgatcc cgaagccggt aacatcgggg tgtggaacaa tgcgttcaag
     6001 gagctgtgtt tttccggcag cgcgctgatc atggcgagct cgttccccga ggacacgagt
     6061 ccccggctcg cggctatcga ccgaccgctg gaaaacctag tcccgatcgg aaccttcttt
     6121 ttttccatca tgctcatcgt tttcggaatg gagcacatgc tgtatggtga atatgtgaag
     6181 gcgctggtcc ccgactggat ccccggtcat cttttctgga cttatttcgc gggggtcgcc
     6241 ctcatcggcg ccggtgtcgg tatcatgttg aggttcaagc tgaggctgat agcgctgctg
     6301 acagggggta tgatctttat ctggtttctg gtgctgcata ttccccgcgc ggtcgtcgca
     6361 ccggtatcgg ataacggtaa tgagctgacc agtgtcttcc aatcgctggg attcagtgga
     6421 gtagcattcg tcatcgcctg cgtgtggccg gcgcggtcgc ggttgaccgc actggcatga
     6481 gtcggcggcc ccgcagttcg ggcgcgagaa gtggcgacct aatggagcat ccccatgcct
     6541 tcgatcctga cgtaagggtt gcggaagaaa tcgcccacca ccgcattgaa ataattcgcc
     6601 ttatacaccg ccacaaaatg cccggacccc ggcaccagcc ataggtacga tttggggatg
     6661 ttccggaata tctgccacag atgataggag ggaatcgcgt cgtggtcccc acccataacc
     6721 agcgtcgggc accggatcgc gtgcagctgt tcgagggtca tatgtggctc cttcagatcg
     6781 agctcgacga tcttaagatc gttgcgggta cgcggatttg gaggcagccg gcgcatacta
     6841 tcggccatgc gctccagcca gtgataaata aacggttcaa gaccagtggt gtccggccat
     6901 agattgggcc ccgaaaccac cagcttcttt actttttcgg gatgccgaag ggccagcaga
     6961 agaccgctga tccctccgtc gctccacccc agcacgaaac agctgtccag gtgaaggctg
     7021 tccagcagcg cattgaaatc gtccgccatc atttcgaatg tcagcgaatc gccggcatcc
     7081 acgctcttgc catgcgcccg ggtgtccacc gcaatgacgc ggtaattttt ggcgaaatag
     7141 ggtatctggt tggtaaagac attgatcgat ccgccattga tgtgcagcat caggagcggg
     7201 gcgccctgcc cgtatgtctc ataataaagc cggatgcccc tggtggacag atagtggcct
     7261 gccgcagcat tgtccccata gaccacctgc gaccgggcgc tgccgcaata gcaggcgagt
     7321 aatataagca gtaggaattt tttgctcata gcatagatgt tgctacgaaa gtacggcttt
     7381 tggacccctt cagatagtga aattaccgtt tgagtagatt ggctggggat gcgctccggc
     7441 aagtcgtaac ttaataaggc taacaacatg atcacctaaa aacaaggtta tgccaaaact
     7501 tactgtcatg agacacactg ccatcctctc cctggccgtt gcctcgctca cgccgctcgc
     7561 ttccaggagc cagatcacca ccccgcgtgt tcccagtcct gccgccgctg tctcacagac
     7621 gatcggcatc tcgacggtca ccgtcaacta ttcccggccc tccgttaaag gcagacaggt
     7681 atggggtacc ctcgtccctt atggatggaa caagcagggt ttcgggctgg gcaacgatgc
     7741 gccatggcgg gccggcgcta acgagaatac ggtcctccat ctgtcgcatg acgcgaaggt
     7801 ggaaggtcac acggtgcccg cgggagacta tggccttttc ttcgttatca ataaagacaa
     7861 tacgggagtt gttgttcttt ctaaggacta ccggtcctgg ggttccttct tctacgaccc
     7921 gcagcatgac gagatgcggg cagacatttc gctacgcgcc attcccgaaa ccgagctgct
     7981 gacctatgat ttccagaatg tcacgaagaa ttccgccgag ctggtctgca attgggaaaa
     8041 gatgcagttc cccgtgaaga tcgaattcgc cgtcgacgat atcgtcatgg ccaatgcggc
     8101 gcaggagctg aaagggacgg tgggtttcaa ctggcagggt ttttccagcg ccgccaatta
     8161 tgcgctgcag aataaggtca actatgatca ggcactgacc tggatcgatc aggcgatcgc
     8221 catcaatccc aatttcaccg atctgcggat caagtcggca ttgctgaagg ccaagggaaa
     8281 agacgccgat gccgacaata tcatgaaaac cgccctcgat gcctctaccg aagtcgagct
     8341 caaccagtac ggctatcagc tgcttgccca gaatcagcag gacaaggcca tcgatgcctt
     8401 cgtgctcaat acgcggcgtc atccgacaag cgcgaatgtg ttcgacagtc tcggcgaggc
     8461 ttatgccctg aagggggata agaagaatgc gatcgtcaat ttcaagaaat cgctgactat
     8521 ggatccgccg gcggcgacaa aggcgaattc ggaaaaatat ctcaaacaac tgggcgcgat
     8581 gtgactgctt tcatgctcaa tgcgcggcgt catccgacga ctcatgtggc ggcaacgacc
     8641 tttgccggct caggcatctt gcgattgcga caataccggt ttgatgtcga cgaccggagt
     8701 accgtcgatg gcttccagcg gtcccacttt tatccggaca ccgtcgatcg caaggacatt
     8761 cacccgatgc agacccaatg ggttcggacg gtctggcgat ctggtggcga aaacaccggt
     8821 cagcggattg cgctcctctc cgcgcggatg cacttttaaa acgttgcggc tcgctcgatg
     8881 aagccacgta atgagaatga tggcatcccc cgggcggata ccatccatcg ccggcgcata
     8941 ttcttttcgc agctcgatcc atgcttccgg ggcggattca tacccttgtt tgggtgcctc
     9001 gtccaggttt ttgagcgggc ttttaatgga acctatgggt tctaggacaa aatcgttcat
     9061 aatactgaat tatttattca cacttagttt cctgcaataa tattgaattt caacttcttc
     9121 gacgggcccg cgtcctgtga cacaggttgt tcgtcgtctg cgggcacacg acactgttgc
     9181 aaatttcatc ctcgccgagt atcttgtatc ccgttaatcc ttccacagag tcgataccct
     9241 ttgagatacc atgatccaat tcattgtatt ccaaacgtaa atgagactct atgttacacg
     9301 caaaagacct tcggttcggg aacaaggtgt tgaaccggag aggtgaagtg atcaccgttc
     9361 agcagatcca cagcaacagc atcgtttatg acacggccat cagcgtcagc aggcgccatt
     9421 tgtccaaggt cagcggatat gacgcgacct atacggcgga ggtgctcgaa gtggtcaagg
     9481 aggccggtct ccgggagatc gaacccatcg ttctgacgcc caaagtgctg gagaagtgcg
     9541 ggttcaggaa ctttatccgc gaggaatgga tcgttactta cggtaataaa catacggact
     9601 tcgaattcac cgaagacggg ttgcggctga aacacccttc gccttcgcgc gtggggatca
     9661 aatacctcca tcagctgcag aatttcttct acgctatcgc aggagaagaa ttgcagatcg
     9721 aactgtagac cgccggcgca aaaaaaacct gccatcggga gatggcaggt tcgatggtcg
     9781 ggaatcgaat ttcctgaggc gacgtcgcag gagcctattt cacattcttg ctgacgatct
     9841 tggcgaggtc gaacatggac acctggtcct tgc
//
LOCUS       DASYSV010000293         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2360553, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000293 DASYSV010000000
VERSION     DASYSV010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360553"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VGQ51_08845"
     CDS             <1..413
                     /locus_tag="VGQ51_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-S]-binding protein"
                     /protein_id="HEV9036715.1"
                     /translation="PIYKNIGGHSYGTTYSGPIGSVITPHLKDMGEWKHLSYASSLCG
                     NCTEVCSVKINLHELLLDNRSQAVREGYATWSEKLAWKFWRKASLSRSLMNMGSAKLK
                     NWVVNRSFKAWTANRSELEFAPKTFNQRWKAERK"
     gene            413..1726
                     /locus_tag="VGQ51_08850"
     CDS             413..1726
                     /locus_tag="VGQ51_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV9036716.1"
                     /translation="MTLYCPDITSRLRWIIGFCSDQLFDVPINITSDRSAFERTSGPR
                     INYSTESIPDAFTIRPAALLFETEIKPQSITCFEYNGHKALFPTGGDLSFDLFAASFY
                     LMSRYEEWLPHEKDVFGRFAHTQSLACREGFLEIPLVNYWLLDLRDALGRKYPDLVFR
                     YTDFKFIPTYDIDSAWSYLHKGWQRNLGGAMKSIIKGEWQAFWNRTRVLQRRLRDPFD
                     AYEWLDSLHLYCRLRPYYFFLVASRRYGVDRNISPSSPALRELIQYHASGYRIGIHPS
                     WRSGDDDRTLREEIEWLEVVAGHPITRSRQHFIRFHLPDTYRQLLKYGIRQDFSMGYG
                     SINGFRASVACSYYWYDLGKEETTDLRIFPFCFMDANSYYEQRYTAPQAMSELMHYYR
                     QIRAVNGIMVTIWHNNFLGSDPAFAGWREVYEVFLKEEIFWYGNS"
     gene            complement(1723..3255)
                     /gene="purH"
                     /locus_tag="VGQ51_08855"
     CDS             complement(1723..3255)
                     /gene="purH"
                     /locus_tag="VGQ51_08855"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV9036717.1"
                     /translation="MDKQIKSALISVFYKDGLEPIVRKLHQSGVVIYSTGGTQQFVEG
                     LGLPCTPVESLTSYPSILGGRVKTLHPSVFGGILGRRDQPSDVEEMSRYQIPEIDLVI
                     VDLYPFEETVAATKDDRSIIEKIDVGGPSMIRGAAKNHKDVAVIASRVQYGWLLNLLN
                     DQNGSTSIEQRRLLAAKAFEVCAHYDVAIARYFSPGGMDYFLESVPSPKSLRYGENPH
                     QSARFFGKIEDLFDQINGKELSYNNLVDVDAAVQLIQEFADDKAPAGNAVFAIVKHTN
                     VCGIAIRPSLKQAWDSALAGDPESAFGGVLCCNLPVDKATANAINEIFFEVLIAPAYD
                     KDALDILRSKKNRILLVQKARITPIRQFRSLLNGVLQQQPDEGNFTEWKEVGGRPAAD
                     GEKADLAFANIVCKHLKSNAIALIKDRQLIGKGCGQTSRIDSVRQAIAKAAQFNFDLH
                     GAVLASDAFFPFNDCVQIAHSAGITAFIQPGGSIRDKDSIEYCVEKGLVMVITGRRHF
                     RH"
BASE COUNT          765 a          894 c          881 g          835 t
ORIGIN      
        1 gtcccatcta taagaatatc ggcgggcaca gctatgggac cacctatagc ggacccatcg
       61 gatcggtgat caccccgcat ctgaaagata tgggggaatg gaaacatctg agctacgcat
      121 cctcgctttg cggcaattgc acggaggttt gttcggtaaa gatcaatctt cacgagctgc
      181 ttctggacaa ccggtcgcag gccgtaaggg aaggatacgc gacatggtcg gaaaagcttg
      241 cgtggaaatt ttggagaaag gcatcgctta gccgtagcct gatgaatatg ggaagtgcca
      301 aactcaagaa ctgggtggtc aacagatcct tcaaggcctg gacggcgaat cgttccgaac
      361 tcgaatttgc ccccaaaaca tttaaccagc gctggaaggc cgaacggaag tgatgaccct
      421 ttattgtcct gatatcactt cacgtctgcg ctggatcatc ggtttttgca gcgatcagct
      481 cttcgacgtt cccatcaaca tcacgagtga taggtcagca ttcgaaagga catccggacc
      541 ccgtatcaac tattccacgg agtccatacc cgatgcattt acgatccggc cggccgcctt
      601 gctcttcgag acggagatca aacctcaatc catcacctgt ttcgaataca acggtcataa
      661 ggcgttgttt cccaccggcg gggacctgtc tttcgatctg tttgcagctt cattttacct
      721 gatgagccgt tatgaagaat ggcttccgca tgagaaagac gtgtttggcc gttttgcgca
      781 tactcagtcc cttgcgtgcc gggaaggctt tctcgaaatc cccctcgtca attattggtt
      841 gcttgatctc cgggatgcgc tgggccggaa atacccggat ctcgtctttc gctataccga
      901 tttcaaattc atcccgacct atgatatcga ttcggcctgg tcgtaccttc ataaaggatg
      961 gcagcgaaac ctgggcggtg cgatgaagtc gatcataaaa ggagaatggc aggcgttttg
     1021 gaaccggacc cgggttttgc aaagacgtct ccgtgatccc ttcgacgcct atgaatggct
     1081 cgactcgcta cacctttatt gccgtctccg gccttattat ttctttcttg tcgcttcccg
     1141 gcgttatggt gtggacagga acatatctcc ttcttcaccc gccctgaggg agctgatcca
     1201 gtatcatgca tccggctatc gcatcggtat ccatccttcc tggcgcagcg gcgatgacga
     1261 tcggacgctg cgggaagaga tcgaatggct ggaagtggtt gccggacatc caatcacccg
     1321 aagccgtcag catttcatac ggttccatct tcccgatacc tatcggcaat tgctgaaata
     1381 tggcatccgg caggatttct ccatgggata tggcagcatc aacggatttc gggcttccgt
     1441 cgcgtgttct tattattggt atgatctcgg aaaagaggaa acgaccgatc tgcgcatctt
     1501 tcctttctgc ttcatggatg ccaactccta ttatgaacaa cgatataccg cgccgcaggc
     1561 gatgagcgag ctgatgcatt actatcgtca gatccgggcg gtcaatggca taatggtcac
     1621 catctggcac aataactttc tgggcagtga cccggccttc gccggctggc gggaagtgta
     1681 tgaagtgttt ttaaaagaag agatcttctg gtacgggaac agctaatgtc tgaaatgtct
     1741 cctccccgtg atgaccatca ccagcccttt ctccacgcaa tactcgatcg aatccttatc
     1801 ccggatcgag ccccccggct ggataaaagc ggtgatgccg gcggaatggg cgatctggac
     1861 acaatcattg aacgggaaga atgcgtcgct cgccaacacg gccccatgca gatcgaagtt
     1921 gaactgggcg gccttcgcga tagcctgtcg caccgaatcg atccggctgg tctgtccgca
     1981 acccttgccg atcagctggc gatccttgat tagggctatg gcattagact tcaggtgttt
     2041 gcagacaata tttgcaaaag cgaggtctgc tttttcgccg tcggcagcag gtcttcctcc
     2101 tacctccttc cattccgtga aattgccctc atccggctgt tgctgcagaa cgccgttcag
     2161 cagactgcgg aattggcgta taggtgtaat ccttgccttt tgcaccagca ggatgcggtt
     2221 ctttttcgac cgtaggatgt cgagcgcgtc cttatcatat gccggggcga tcagtacttc
     2281 gaagaatatt tcgttgatgg cattggcagt cgccttatcc accggcaggt tgcagcaaag
     2341 caccccgccg aaggcgcttt cggggtcccc cgccagcgca ctgtcccagg cttgtttcag
     2401 ggaagggcga attgcaatgc cgcaaacatt ggtatgcttg acaatggcaa aaacggcgtt
     2461 cccggcgggg gctttatcgt ccgcaaactc ctggatcaac tggaccgcgg catccacatc
     2521 cacgagatta ttatacgaaa gttccttacc gtttatctgg tcaaaaaggt cttcgatctt
     2581 gccgaaaaaa cgcgccgatt gatgcgggtt ttcgccatac ctcagcgatt tgggcgatgg
     2641 gaccgattcg aggaaataat ccatgccccc gggactaaaa taccgggcaa tggcgacgtc
     2701 gtaatgtgcg caaacttcga aggcttttgc ggccagtagt ctccgttgtt cgatgctggt
     2761 cgagccgttt tgatcgttca gcaggttgag caaccagcca tattgaactc tggatgcaat
     2821 gacggccacg tccttatggt ttttggctgc cccgcggatc atcgaggggc caccgacatc
     2881 tattttttcg atgatggatc tatcgtcttt ggttgccgct accgtctctt cgaaaggata
     2941 aagatcgacg atcacgaggt cgatctcggg gatctgataa cgcgacattt cttccacatc
     3001 cgagggctgg tcccttcttc caaggatacc tccaaatact gacggatgca gggtcttgac
     3061 ccttcctccc agaatggaag ggtaggaggt aaggctttcc acgggagtac agggtaagcc
     3121 caggccttct acaaactgct gggtgccacc ggtagagtag atgaccactc ccgactgatg
     3181 aagctttctg acgatgggtt ccagtccgtc cttataaaag acggatatca gagccgattt
     3241 gatctgctta tccataagag cagcgaaagt acgaaaacgg caggaaaaaa ccggccaggg
     3301 ccggtttcag tttttgaatg gttacagtag gcaagcatag tccggcgagg ttctctcatg
     3361 cgtactaata cgttc
//
LOCUS       DASYSV010000294         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_57272, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000294 DASYSV010000000
VERSION     DASYSV010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57272"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VGQ51_08860"
     CDS             complement(<1..682)
                     /locus_tag="VGQ51_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV9036718.1"
                     /translation="MTRRAYRKAVPLMGNRFELTVISDDETWAMDRINNGIAEIRRIE
                     RLLTTFSEDSETSLINRNAGVGPIPVSSETFDLIERSIRISRLTQGAFDITYGSIDRR
                     LWNFDVTMTRLPDKQTARKMIRLVNFRNIILDRDRQTVFLKENGMRIGFGGIGKGYAA
                     EQAKKVMMASGVDSGIVNASGDLAVWGHQPDGSPWTIGIVDPNARGQIFSYMNVTNMA
                     VATSGNYEK"
     gene            complement(702..1145)
                     /locus_tag="VGQ51_08865"
     CDS             complement(702..1145)
                     /locus_tag="VGQ51_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV9036719.1"
                     /translation="MMKTLVCMVLWACFMTGGWLYNLDEARQLARDQHKNILLNFSGS
                     DWCGPCIELRKEIFDDPGFLQMADSDLVLVNADFPRNKRDQLSKEQQALNNAMADKYN
                     PQGKFPYTLLLNADGKVLKTWEGFPNEKPADFAADVRAQLERHDD"
     gene            1410..>2586
                     /locus_tag="VGQ51_08870"
     CDS             1410..>2586
                     /locus_tag="VGQ51_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEV9036720.1"
                     /translation="MLVLCARTSRAYSILAHEALIDSCWKSYIQPLLLLKYPGSTQAQ
                     LKMAHAYAYGGAITPDIGYFPFGKPFFTNLVHYVRSGDFVNALIDDAKDINEYAFALG
                     FLCHYMADKYGHSLATNKCVPILYPKEKKKFGDYVTYEDDHLSHKRTEFGFDVLQVEE
                     GDYAKLAYHNFIGFEVSDSLLIRAFPEVYGLDIHDIFPDFELAIGTLRWSFKRLFPAL
                     TKAAWLIKNKELKKAHPGMTRKSYVYSMKKTDYYKEFGNNRQKPGLFSAIIAFFVEIV
                     PKIGPLSPLKFKAPGPEVVKMYHSSFDTALQKYEAALKAFRTGTRRLANIDFDTGDFT
                     TPGEYGLADKNYDLLVAALKKNKFRLLNDGLRHNIIAFYSHRSAPTGSRKVQNAWART
                     "
BASE COUNT          620 a          716 c          612 g          638 t
ORIGIN      
        1 atttttcata gttgccggac gtcgctacgg ccatattcgt gacattcata taggaaaaaa
       61 tttgcccgcg ggcgttggga tcgacgatgc cgatggtcca gggactgcca tcaggctgat
      121 gcccccatac tgccagatca cccgaggcat tcacgatgcc gctatcgacg cccgaggcca
      181 tcatgacctt ttttgcctgc tcggccgcat atcccttacc gatgccccca aacccgatgc
      241 gcatcccatt ttccttcaga aacaccgtct gccggtcccg gtccagtata atgttccgaa
      301 aattgaccag cctgatcatc tttcgggccg tttgcttatc cggcagcctg gtcatggtca
      361 cgtcgaaatt ccatagccgc cggtcgatcg aaccgtacgt gatatcaaag gctccctggg
      421 tcaaccggga gatgcggatg gaccgttcga tcagatcgaa ggtctcactg ctcacgggga
      481 tgggacctac cccggcattc cggttgatca aactcgtctc gctatcttcg ctaaaggtcg
      541 tcagcagacg ctcgatccgc cggatctcgg caataccgtt attgatccgg tccatggccc
      601 atgtctcgtc atccgaaatg accgtcagtt cgaatcggtt gcccatgagc ggcaccgcct
      661 ttcgatacgc ccgtcttgtc atggttccta tggatggaga gtcaatcatc gtgccgttcc
      721 aattgcgccc ggacatccgc agcgaaatcg gccggttttt cgtttggaaa tccttcccag
      781 gtcttcaaca ccttcccatc cgcgttcagc agcaaggtgt acggaaactt cccctgtgga
      841 ttatatttat cggccatcgc gttattcagg gcctgttgct ccttgctcaa ctgatcccgt
      901 ttgttccttg gaaaatccgc gttgacgagg acaaggtcgc tgtccgccat ttgcaggaat
      961 cccggatcat cgaagatttc ttttcgtaac tcgatacaag gcccgcacca gtcgcttccg
     1021 gagaagttca aaaggatatt cttgtgctga tcgcgcgcca gctgtctggc ctcatccaga
     1081 ttgtaaagcc agccgccggt cataaaacat gcccacaaca ccatacacac caatgttttc
     1141 atcatatgaa tccagtttta atacagaaag tcgtcgacca ctggccgcca ttattgcttt
     1201 acaaatatta ttccgggacc ttcgccgcac acaattgctg ccggtttcct ttttcccggc
     1261 aatacgaact acggatcagt ctcatgtttt gagccgtact ctaacggaca tgcgaggaaa
     1321 aggccaaaaa attcaggcaa ggtctttgga tgatcaaaaa catgtgtatc cccctaaaaa
     1381 gaacgtgtac ccgggcgatg atctgcctga tgttggttct ttgcgcccga acatcacgcg
     1441 catattcgat ccttgcccat gaagcgctga tcgattcctg ctggaaatcg tacatacagc
     1501 cgcttctttt attgaaatat cccggatcga cccaagccca gctaaagatg gcgcatgcct
     1561 atgcttatgg cggggccata acgcccgaca tcggctattt tccatttggc aaaccgttct
     1621 ttacgaacct cgttcactac gtcaggagcg gggattttgt caacgccctg atcgacgatg
     1681 caaaggatat caatgaatac gcattcgcac tcggatttct ttgccattat atggccgata
     1741 aatatggtca ttcgctggct accaacaaat gtgttcccat cttatatcca aaggaaaaga
     1801 aaaaattcgg cgattacgtg acctatgagg acgatcacct ctcacacaag cgaacggagt
     1861 ttggttttga cgtattgcag gtagaggaag gcgattacgc aaagctcgcc tatcataatt
     1921 tcatcggctt tgaggtttcc gactcgctgc tcataagggc ttttcccgag gtctatggac
     1981 tggatatcca tgatattttt cccgatttcg agctggctat cggaacatta aggtggtcgt
     2041 tcaagcggct gttcccggcc ctgaccaagg ccgcatggct catcaaaaac aaggagctca
     2101 aaaaggccca tcccggaatg acgagaaaaa gttacgtgta ttctatgaag aagaccgact
     2161 actataaaga gttcggcaac aaccggcaga aacccggact cttctccgcc atcatcgcgt
     2221 tttttgtcga gatcgtgcct aagatcggcc ctttgagccc gctgaaattc aaagctcccg
     2281 ggccggaggt ggtgaaaatg taccatagca gcttcgatac ggcgttacaa aaatacgagg
     2341 cggccctgaa agcgttccgc accggcacca ggcggctggc aaatatcgat tttgataccg
     2401 gtgacttcac gacacccgga gaatacggtc tcgcggataa aaattatgac ctgttggttg
     2461 ccgcgttgaa gaagaacaaa ttcaggctcc tgaacgacgg actcagacac aatatcatcg
     2521 ccttttattc gcatcgttcg gcacccaccg gctctcgtaa ggtgcagaat gcgtgggcca
     2581 ggacgg
//
LOCUS       DASYSV010000295         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_57498, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000295 DASYSV010000000
VERSION     DASYSV010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57498"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..>2614
                     /locus_tag="VGQ51_08875"
     CDS             190..>2614
                     /locus_tag="VGQ51_08875"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036721.1"
                     /translation="MRKPILLCLGSALFCLFATAQKISLEQFKDLKPRNIGPAGMSGR
                     ITTIDVVLENPNIIYLGSASGGVWKTENNGNTWTPVFDDQPTQNIGVVAIQQSNPNIV
                     WVGTGEGNPRNSVNIGEGIFKSLDAGKTWKRMGLENTRNIHRIIVDPVNPNTVYVAAI
                     GNPYGIHPERGVFKTTDGGDTWTKILYTNDTSGCSDLVMDPSNPNRLLASMWQHHRTP
                     WDFKSGGPGSGLYLTIDGGKNWKKLGKDDGLPEGQYGKIGLAFARNQPSRVYAMVEAT
                     KNGLYKSDDGGARWQLVTNDPRVVTNRPFYFQDIRVDPQNENRIYNIHDQVEVSEDGG
                     KSFATLIPYSGIHPDHHAWWIDPGNGSFMIEGNDGGIGITHDRGRNWSFDDKIPVGQF
                     YHINTDNEMPYHVMGGMQDNGCWHGPAYTWISGGIRNDYWDNIGGGDGFEVMPDAEDA
                     NWVYSESQGGNAERRNWVTGESWFIKPPSLDPKLMLRFNWNTAMAQDPFDKKTIYFGS
                     QFLHKSLNKGVSWETISPDLTTNDTTEIKAFQNTGGLTLDITGAETHCTIITISPSAK
                     QQGTIWVGTDDGNVQLTRDGGKTWENFRGKIPGMPSGCWIPQITASRYNAGEAFVVAN
                     DYRRGDFKPYIFRTTDYGKTWKKMVDENKVRGYALCMIQDPVEPNLVFVGTENGLWIS
                     FDDGSTFQQWKNGYPSVSTYDLAIQDREADLVVATFGRSLWVFDDIRPFRKMAAGKGN
                     AITNTITVFPAPDAYQAQFRSATGYEWSVDGLYEAENRRRGAEISFFINKPKGQKAKA
                     DSQQMQPTAA"
BASE COUNT          731 a          617 c          717 g          549 t
ORIGIN      
        1 ctttttcata aattatcatt tttagtgagc aaatgcgatg aatcaatgca aaagaccggc
       61 cagggggaag cggcgccccg gggaaggaga aatatcgttg aaaatcaaat acttgaaaga
      121 aaccgggagt gaggagatat ccggccaata tttttcgata gatttaaatc gtcaaaatca
      181 tcacgctcaa tgagaaaacc cattctgcta tgcctcggca gcgccctgtt ctgtctgttt
      241 gcaaccgctc aaaaaatcag tctcgaacaa ttcaaggacc tgaaaccgcg caatatcggc
      301 cctgccggta tgagtggccg gatcaccact atcgatgtcg tactggagaa tcccaacatc
      361 atttatctcg gctcggcatc gggcggtgtg tggaagacgg aaaacaacgg caatacgtgg
      421 acgccggttt tcgatgatca gccgacacag aacatcggtg tcgttgccat tcagcaatcg
      481 aatccgaata tcgtctgggt cggaacagga gaaggaaatc caaggaactc ggtcaatatc
      541 ggcgaaggga tctttaaaag tctcgatgcc ggcaaaacat ggaagcggat ggggttggaa
      601 aatacaagga acatccaccg tatcatcgtt gaccccgtga atcccaatac cgtctatgta
      661 gccgccatcg gaaaccctta tggcatacat ccggaaagag gggtctttaa aacgacggat
      721 ggcggcgata cctggacaaa aattttgtat accaatgaca cctccggctg ctccgatctg
      781 gtcatggacc cttccaatcc taatcgtctg ctcgcgagca tgtggcaaca tcaccgtacg
      841 ccatgggatt ttaaaagcgg ggggcccggc agcggattgt atctgaccat cgatggcggt
      901 aagaattgga aaaaactggg caaggacgac ggtttgcccg agggccaata cgggaagatc
      961 gggctggcgt ttgccagaaa ccagccaagc cgcgtgtatg cgatggtgga ggcgacgaaa
     1021 aacgggttat ataaaagcga tgacggcggc gccaggtggc agcttgttac caacgaccca
     1081 agggtcgtaa cgaatcggcc tttctatttt caggacatac gcgtagaccc gcaaaatgaa
     1141 aaccggatct acaatatcca cgaccaggtg gaggtaagtg aagacggcgg taagtcgttc
     1201 gccacattga tcccttattc gggcatacat ccggatcatc atgcctggtg gatagatccg
     1261 gggaatggaa gctttatgat agaaggtaac gacggcggca tcggtatcac gcacgatcgg
     1321 ggcaggaact ggtccttcga cgacaagatc cctgtcggtc aattctatca tatcaatacc
     1381 gacaatgaaa tgccgtatca tgtcatgggc ggcatgcagg ataacggatg ctggcacggt
     1441 cccgcctata cctggatatc cggcggcatc aggaacgatt attgggataa catcggcggc
     1501 ggggatggct tcgaggtaat gccggatgcc gaggatgcca actgggtata cagtgaaagc
     1561 cagggcggaa atgccgaacg tcgcaactgg gtgacgggag agagctggtt tataaaaccg
     1621 ccttccctgg atccgaaact gatgctgcga ttcaactgga atacggcaat ggcgcaggac
     1681 ccgttcgata aaaagacgat ctatttcggc agccagttct tgcataagag cctgaataaa
     1741 ggggtaagct gggagaccat ctctcccgat ctgaccacca acgatacgac ggagatcaaa
     1801 gcatttcaga acaccggcgg gctgacactg gacattaccg gcgcggaaac gcattgcacg
     1861 atcattacta tttctccctc ggccaaacag caagggacta tttgggtagg aacggacgat
     1921 ggtaatgtgc aattgacaag agacggcggt aagacatggg aaaacttccg cggaaagatc
     1981 ccgggtatgc catcgggttg ctggataccc cagataacgg catcccgtta taatgcaggc
     2041 gaggcattcg tcgtagctaa cgattatcgc cgtggcgatt tcaaacctta tatattccgt
     2101 accaccgatt atggcaagac ctggaagaag atggtcgatg aaaacaaagt aaggggttat
     2161 gcgctttgta tgatccagga cccggtagag ccgaatcttg tctttgtcgg gaccgaaaac
     2221 ggcctatgga taagttttga tgacggatcg acctttcagc aatggaaaaa tggttatcct
     2281 tctgtttcta catatgatct ggccatccag gacagagagg ccgatttagt agtcgcgaca
     2341 ttcggacgtt cgctatgggt attcgacgat atccgtccat tcaggaagat ggcggcgggt
     2401 aaagggaatg ccatcaccaa cactatcacg gttttcccgg cgccggacgc atatcaggct
     2461 cagttcagat cggctaccgg ttatgaatgg agtgtggatg gtctatacga agccgaaaac
     2521 aggagacggg gggccgaaat ttcattcttt atcaacaaac cgaagggaca aaaagcaaaa
     2581 gcggatagcc agcaaatgca gccaaccgcg gccc
//
LOCUS       DASYSV010000296         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_696859, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000296 DASYSV010000000
VERSION     DASYSV010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696859"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VGQ51_08880"
     CDS             <1..209
                     /locus_tag="VGQ51_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV9036722.1"
                     /translation="LLAIGLFTRFAALVACFSMCVAEFWAHHGHVATMNGMLHEQAWL
                     YLTAFFAILLVGPGRISVDGAMGK"
     gene            222..806
                     /locus_tag="VGQ51_08885"
     CDS             222..806
                     /locus_tag="VGQ51_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036723.1"
                     /translation="MKKIDIIQTTLIIIAVLNAYNALYTIIALVTEVPMVSGPGGQQA
                     LIAQFLISALVTSGVFLIIRYSRPIAAWLLRFDPEGDAEGSARWHLDRRTLIFALFIG
                     IGALMLINALPDVAADLFYAFKSKVSRSVEPFAPNENQLLLQLLKVALGALIVYAAPG
                     LTGFIEKSIAVRLKGQPSEIAAVDPDPEPETQSS"
     gene            808..1218
                     /locus_tag="VGQ51_08890"
     CDS             808..1218
                     /locus_tag="VGQ51_08890"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV9036724.1"
                     /translation="MYTHETIVRVRYAETDQMNVVYHGNYAQYFEVGRVEAIRALGIS
                     YKDLETMGIIMPVIEWTAKFLRPAHYDDLLTVRTTLKEWPKDHRIEFLQEVYNEKGKL
                     LTSGKVLLYFLLSATMEKTSMPPEMEEKLRALFL"
     gene            complement(1207..2592)
                     /gene="dacB"
                     /locus_tag="VGQ51_08895"
     CDS             complement(1207..2592)
                     /gene="dacB"
                     /locus_tag="VGQ51_08895"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEV9036725.1"
                     /translation="MMYRFTILLFCLFMGKFAYPQRPSLEGRLREAMDRLEKDSSMRH
                     AIVSLCVLDGGTGTVLFERNSQVGLAPASCQKLFTSVAALDLLGPSYRYKTRLGYKGK
                     ITGGLLHGDLVLAGSGDPTLGSWRFDSTKEEVVLGRWIRAIRSAGITSYDGSIVVDEG
                     AWGTDAVPGGWIWEDIGNYYGAGAWGLNWHEDQYDVRLQAGKAVGDPVTILNQSDLER
                     TVCPTWVNELKTGPAGSGDNAYIYLPPNGNVAFVRGTAPLQKEERVISGSLPSPSRQV
                     CVLLAEALTKKEGAPSAAGDTVWIGGSDSPPLDSINYWFLRRSINLYGEALIKTLALE
                     KTGVASTEGGVTVVQDFWSGHGIDREALHIIDGSGLSPQNRVTTDALAHVLEYARSRP
                     WYHSFYNSLPMFNGMHMKSGSIGGARSFSGYQMGADGKEYVFSIIVNNYDGSSGEAVR
                     RLYEVLDILKK"
     gene            2625..>3235
                     /locus_tag="VGQ51_08900"
     CDS             2625..>3235
                     /locus_tag="VGQ51_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV9036726.1"
                     /translation="MENIVASIITIGDELLIGQVVDTNSAWMGQELNKNGIWVAHRVA
                     VGDTREDILAALDDESRRSQIILITGGLGPTADDITKPILLEYFGGNMVVDETALANV
                     KGIFEKLKRPMIERNLRQAEVPDTCTVISNRRGTAPGMWFEKDGLIYVSMPGVPHEMK
                     GMMTDDVLPAIQKRFSLPFIAHRTLLTAGVGESFLADHIRSFE"
BASE COUNT          774 a         1061 c          725 g          675 t
ORIGIN      
        1 tcctgcttgc gatcggtctc ttcacccgtt ttgccgcgct tgtagcctgc ttcagcatgt
       61 gcgtagccga gttctgggcc caccacggcc atgtcgcaac gatgaatggc atgctccacg
      121 aacaagcctg gctctacctc accgcctttt tcgccatcct cctggtcggc cccggccgca
      181 tcagcgttga cggcgccatg ggcaaataac acacccgacg catgaaaaag atcgatatca
      241 tccagacgac cctgatcatc attgccgtcc tcaatgctta taatgcattg tataccatta
      301 tcgcattagt taccgaagta ccgatggtat cagggccggg cggccagcag gcactaattg
      361 ctcaatttct aatttccgcg ctggtgacaa gtggcgtatt cctgatcatc cgctacagcc
      421 ggccaattgc cgcctggttg ctcagattcg atccggaagg cgatgcggaa ggttcggccc
      481 gatggcatct cgaccgtcgt acgctgattt ttgcgttgtt catcggcatc ggggcattaa
      541 tgctcatcaa tgcattgccc gatgtggcag cggatctttt ttacgcgttc aaaagtaagg
      601 tcagtcgcag tgtagaacca tttgctccaa atgaaaatca acttttgctc cagcttttga
      661 aagtcgcact tggtgcctta atcgtttacg cagcgccggg cctcaccggt tttatcgaga
      721 aaagcattgc ggtcaggctg aaaggccagc cgtcggaaat tgcggcagtc gatccggacc
      781 ccgaacccga gacacaatcc agttaatatg tacacccatg aaaccattgt ccgcgtgcgc
      841 tacgcggaga ccgaccagat gaatgtcgtc taccacggca actacgccca atacttcgaa
      901 gtaggtcgcg tggaagccat ccgcgccctc ggcatcagct ataaagacct cgaaacaatg
      961 ggcatcatca tgcccgtcat cgaatggacc gccaaattcc tccgccccgc ccattacgac
     1021 gatctgctca ccgtccgcac gaccctcaaa gaatggccca aagaccaccg gatcgaattc
     1081 ctgcaggaag tctacaacga aaaaggcaag ctcctcacct ccggcaaggt cctcctctat
     1141 ttcctcctgt ccgccaccat ggaaaaaacc tcgatgccgc ccgagatgga agaaaagcta
     1201 cgggccctat ttctttagaa tatccaatac ttcatacaac cgccggaccg cctcccccga
     1261 cgacccgtca taattattca cgataatact aaacacatac tccttcccgt cagcccccat
     1321 ctgatacccc gaaaaacttc ttgccccgcc gatcgatccg ctcttcatat gcatcccatt
     1381 gaacatcggc aatgaattat aaaaggaatg ataccacgga cggcttcggg catattccag
     1441 gacatgcgcc agcgcatccg tcgtcacccg attttgcggg gaaagaccgc tcccatcgat
     1501 gatatgcagc gcctcacggt caatgccatg cccgctccag aaatcctgca ccaccgtaac
     1561 gccgccttcc gtggaggcga cacccgtctt ttccagcgcc agcgtcttga tcagggcctc
     1621 cccatacaga ttgatactcc gccggaggaa ccagtaattg atgctgtcca gcggcggcga
     1681 atccgaccct ccgatccaaa ccgtatcgcc ggccgccgac ggagccccct ctttctttgt
     1741 gagtgcctct gctaataaga cacacacttg ccgtgacggc gacggcaacg aaccggagat
     1801 caccctttcc tctttttgca agggcgccgt cccccggaca aatgccacat tcccgttcgg
     1861 aggaagataa atataggcgt tgtcgccact gcctgccggt ccggtcttca actcattgac
     1921 ccatgtcggg cacaccgttc tctccaggtc cgactggttg agaatggtca ccggatcacc
     1981 caccgccttc ccggcctgca gccggacatc atattgatcc tcgtgccagt tcagccccca
     2041 ggcgcccgct ccataataat taccgatatc ttcccatatc cacccacccg gaacggcatc
     2101 cgtcccccat gcgccctcat ccactacaat ggacccgtca tagcttgtga tcccggcact
     2161 ccgtatcgcc cgtatccacc tccccagcac aacctcttcc ttcgtcgaat caaaccgcca
     2221 actacccaat gtcggatctc cactacccgc caggacaaga tcgccatgta atagtcctcc
     2281 ggtgatcttc cccttataac ccaaccgggt cttataccga tacgaaggcc ccagcagatc
     2341 aagcgcagct acactggtaa agagcttttg acaactggcc ggcgccagcc cgacctgcga
     2401 atttcgctcg aagaggacag tccccgtccc cccatccaat acacaaaggc tgacaatagc
     2461 atgccgcatg gacgagtctt tttccagccg gtccatcgct tcgcgaagac ggccttcaag
     2521 ggacggccgc tgcggatacg cgaacttccc catgaacaaa cagaacaaca ggattgtaaa
     2581 tcgatacatc ataacagcta atttaaataa attagctttg ccaaatggaa aacatcgtcg
     2641 ccagcatcat caccatcgga gatgaacttc tgatcggaca ggtcgtagac accaacagcg
     2701 cctggatggg acaggaactg aataagaacg gcatctgggt agcccatcgg gtagccgtag
     2761 gcgatacccg cgaagacatc ctggcagctc tcgacgatga atcccgccgc tctcagatca
     2821 tcctcatcac cggtggcctc ggccccaccg ccgacgacat cacaaaaccc atcctcctcg
     2881 aatatttcgg cggtaatatg gtcgtcgacg agaccgccct tgccaatgtc aaagggatct
     2941 tcgagaagct aaaacgcccc atgatcgaac gcaacctcag gcaagccgaa gttcccgaca
     3001 cctgcaccgt tatctcaaac cgccgcggca ccgcaccggg tatgtggttc gaaaaagacg
     3061 gcctcatcta cgtttccatg cccggcgtac cccatgaaat gaaaggcatg atgaccgacg
     3121 acgtcctccc ggcgattcaa aaacgcttca gcctaccctt catcgcacac cgcaccttgc
     3181 tcacagccgg cgtaggcgaa tcttttctgg ctgaccatat ccggtcgttc gaaga
//
LOCUS       DASYSV010000297         5461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1589444, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000297 DASYSV010000000
VERSION     DASYSV010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589444"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1166)
                     /locus_tag="VGQ51_08905"
     CDS             complement(78..1166)
                     /locus_tag="VGQ51_08905"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9036727.1"
                     /translation="MKKKNRFSFWKISLWLAGINLFWLILALVMIKYVKFPYQTWNPQ
                     SFNTPFFLLRMGVSLVFQCFYYYFALNWYYRLIARRASLKRFALASLLLFIACITYAG
                     LNDLTIQKEELKVVLDISLKAFAYGISGLFHILTALAIAAIARQLDEKRDQAENQRVL
                     EQRSSRLEREKLQADYLFLKAQINPHFLHNTLNFLYARALPLSPELSEGILTLSEIMR
                     YSLDHREEEDGKVLLTRELEHVHNVIRLQQLRFGHSLQVRFVLKGDPAGLRILPFILI
                     TLVENAFKHGDLRDPDNPAKIELDVSGPGSLHFICSNRKKGGPKELSTGIGLDNSRRR
                     LELTYGENYSLYIKDQLDFFTVDLTITL"
     gene            complement(1178..1873)
                     /locus_tag="VGQ51_08910"
     CDS             complement(1178..1873)
                     /locus_tag="VGQ51_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9036728.1"
                     /translation="MITINKLTKAYNGKVVVNIPTLDIKEGELLGLVGNNGAGKTTLF
                     RLLLDLVRADAGEVFSKGQPVSTTEEWKSYTAAYLDEGFLIGYLTPEEYFYFVGNLHG
                     VSRSNVDQFLRGLDDFFAGSVLKTGKYIRDLSKGNQFKTGIAACLLADPELVILDEPF
                     ANLDPSSQMRLIRMLKDVCAKKRTTVLISSHDLNHVTDVCHRILLMEKGRIIKDTDTT
                     ADTLQELEAYFQV"
     gene            complement(1884..3359)
                     /locus_tag="VGQ51_08915"
     CDS             complement(1884..3359)
                     /locus_tag="VGQ51_08915"
                     /inference="COORDINATES: protein motif:HMM:NF039827.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HEV9036729.1"
                     /translation="MILTLLSHQWKSFWRRRGAGRNLALQLLFGFMILYLLGCALFLG
                     FALKHLLQQLLPHQDIIDTFCSFILYYFGIDIILRLAMQELPVLTVTPYLSQNIRRRQ
                     LVSFLNIRSLFHFFNFLPLILFFPFIFTVILPVYGWPTTIAFTIAILSLTAFNHFLLL
                     YIKRKLIINSWWLIGFIGLLLVFCILDRMKILPVRNISTTVFTRCLHLPWLVVVPILA
                     AMLAFVVNRRMLLRNLYVEEIRSAQKSRRSLDYAWLRHWGAVGDLMALDFRLIARNRR
                     PRSVVLIAVAFLMYGFIFYQPRNLDPMRFGLMLFCGLFMTGLFLINYGRWGFAWQSGH
                     FDGLLASNLSVEEYIRGKATLYTLGTTLAFIITTAYGFIDRRIVPIEAAAWLYNIGVT
                     VPLSLWASMFNSRAIDIDKSAMFNYQGTGGAEMLYSVIVFLSPFAIYLPVSLVFNSWT
                     GIAAIAAAGAIGLLFRGRLIVLLKKEFYKRKYLMLQGFREK"
     gene            3505..4398
                     /locus_tag="VGQ51_08920"
     CDS             3505..4398
                     /locus_tag="VGQ51_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV9036730.1"
                     /translation="MMARIGRIIVRTILALLIFFITLLLVFDRLVQFRMDNSELLTWF
                     SGRHIQPKIGYYKTEGRQIRYVQVGDSPDATILFIHGAPSSLSYWKSYLSDSTLLSRA
                     TMFAADRPGYGYSGLADPLPDIAQQAAAQVPMLDSLHKAHHPIIVVGVSYGSPIACRL
                     AMDHPELVDGLVLVAPPLGPGQERMFWFTYAIESPLIHWVVPRMLQTANQEKVHHKEE
                     LTKMLPLWGRIHVPVIYLQGEKDGLVDTTNATFAREHLTNVPYLDIRMIPGRGHLIAF
                     AEKDRIEKAILQILDKTENRK"
     gene            complement(4407..>5461)
                     /locus_tag="VGQ51_08925"
     CDS             complement(4407..>5461)
                     /locus_tag="VGQ51_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV9036731.1"
                     /translation="MIVNTIDLFNEDPVDARALYCHYFNDLPSEFYISGIDGEKAFTA
                     FSEKYAARIARVFKYEWYDKEKKKVCFWRALAVLDNQCVAEFDQSVCELWHNDREAAF
                     LIEAANLIAPFKQRQRREALEINVVIRGNNRLELKAMEIKRTRLDLGLYYNPGFREID
                     TVIRQRLNKSKDKGIVLLHGIPGTGKTTYLRYLISRIKKRILFVSPAVTNDLMNPDFI
                     QLLTSNPDTVLIIEDAENVIMDRRHNPGSSVSNLLNISDGLLSDFLSVQLICTFNSPL
                     TLVDSALLRKGRLIAKYEFGKLDVAQSQRLSNHLGFDTIITSPMTVAEIAGQHERQVE
                     PERMEILGFRSHLATN"
BASE COUNT         1351 a         1349 c         1473 g         1288 t
ORIGIN      
        1 gggcaggcaa ttgccgcaca taatgctcga gcacatcgat cgcgtgctgt tcgtcatcca
       61 cgatgataca ggtcatgtta caaagttata gtaagatcga cggtaaagaa atccagttga
      121 tctttaatat ataaagaata attctctcca taggtgagct cgagccgccg gcggctgttg
      181 tcgagaccga tgccggtcga aagttctttg ggcccaccct tcttcctgtt gctgcagatg
      241 aaatgcaggc tgcccggtcc ggatacgtcc agctcgatct ttgcagggtt gtccgggtcg
      301 cggagatcgc cgtgtttaaa ggcgttctcg acgagggtga tcagaatgaa tggtagaatg
      361 cgcaaacccg caggatcgcc tttaagcacg aaccggacct gcaggctgtg accaaaacgt
      421 agctgttgga gacggatgac attgtgtaca tgctcgagct cgcgtgtgag cagcaccttc
      481 ccgtcctctt cctcgcgatg gtccagcgaa taccgcatga tctccgatag ggtgagtatc
      541 ccttcagaaa gttcggggga caagggcagc gcgcgggcat agagaaaatt cagcgtattg
      601 tgcaggaaat gaggattgat ctgtgccttg agaaacagat aatcggcctg tagcttttct
      661 ctttccaacc gggaagagcg ttgctcgagc acccgctgat tttcggcctg atcccttttt
      721 tcatccagct gacgcgcgat cgcggcgatg gccagggccg tgagaatatg gaaaagaccc
      781 gatataccat aggcaaacgc ctttaggctg atgtccagga ccacctttag ctcttccttt
      841 tggatagtta ggtcgttgag accggcatag gtgatgcagg ctatgaagag gagcagcgag
      901 gcaagagcga atcttttcaa ggatgccctg cgggcaatca gccggtaata ccaattcagg
      961 gcaaaataat aataaaagca ctggaatacg agcgatacgc ccattcgcag taggaaaaag
     1021 ggtgtattga atgactgcgg gttccatgtc tggtagggga actttacata tttgatcatc
     1081 accagcgcca ggatgagcca gaacaggttg atgcccgcca gccacaatga gatcttccaa
     1141 aaggagaaac gattcttttt cttcatccgg attgattcta aacctggaaa taggcctcca
     1201 gttcctgaag tgtatcggcc gtcgtatcgg tgtccttgat gatcctgccc ttttccatca
     1261 gcaggatacg atgacatacg tcggtaacat ggttcaggtc atgactgctg atcaggacag
     1321 tggttctctt ttttgcgcaa acatccttca gcatgcggat cagccgcatc tgcgaagagg
     1381 ggtccaggtt ggcaaagggc tcatccagga tcacgagttc cgggtctgca agaagacagg
     1441 ctgcaatgcc ggtcttgaac tggttgcctt tggacagatc gcgtatgtat ttccctgttt
     1501 tcagaacgct acctgcgaaa aaatcgtcca gaccacgcag gaactgatct acgttagacc
     1561 gggagacccc gtgcaggttt ccaacaaaat aaaaatattc ttccggtgtc agatagccga
     1621 tgaggaaacc ctcgtcgaga taagcggctg tatagctttt ccattcttcg gtagtgctga
     1681 ccggttgtcc tttcgagaag acctctccgg catccgccct gaccagatcg agcaggagtc
     1741 tgaacaacgt ggtcttgccc gcaccattgt tgccgaccaa cccgagtaac tctccttctt
     1801 tgatgtccag cgtaggaata ttgaccacga ctttgccgtt atatgctttg gtcagtttgt
     1861 tgatcgttat catttttgag ggtttatttt tctctgaagc cttgcagcat caggtattta
     1921 cgtttataga attctttttt cagcaggacg atcaaacgtc cgcggaacag cagtccaatc
     1981 gcgccggctg cggcgatcgc ggcgataccc gtccaggaat tgaaaacgag cgacactggc
     2041 agatagatcg cgaagggcga tagaaaaacg atcaccgaat acaacatctc tgcgccgccg
     2101 gtaccctgat aattgaacat tgcgctctta tctatatcga tagcccggga gttgaacatc
     2161 gaggcccaga gactcagcgg aacggtcacg cctatgttat acagccatgc ggcggcttcg
     2221 atagggacga tcctacggtc gatgaaaccg taggcggtgg tgataataaa tgccagggtg
     2281 gtgcctagcg tatagagcgt ggctttaccg cggatatact cttcgacgga aagattggac
     2341 gcgagcagac cgtcaaaatg tccgctttgc caggcaaaac cccaccgtcc gtaattgatc
     2401 aggaaaagac ccgtcatgaa cagaccgcag aaaagcatta gaccgaatcg catgggatcg
     2461 aggttcctgg gctggtaaaa tataaatcca tacataagaa aagcaactgc aatcagcact
     2521 accgatcttg gccgccggtt cctggcgatg agacggaaat ccagggccat caggtcgccg
     2581 acggctcccc aatggcgcaa ccatgcataa tcgagtgaac gcctgctttt ttgtgccgac
     2641 cggatctcct ccacgtacag gttgcgaagg agcatgcgcc ggttgactac gaatgccagc
     2701 attgccgcca ggatcgggac caccaccagc cagggaagat ggagacatcg ggtgaagacc
     2761 gttgtggata tattccgtac cggaaggatc ttcatacggt caaggataca aaataccagg
     2821 agcaacccga taaacccgat aagccaccag ctgttgataa tgagctttcg tttaatgtat
     2881 aacaggagaa agtggttgaa ggccgtgagc gacaggatcg cgatcgtgaa ggcgatcgtg
     2941 gtcggccaac cgtagacggg aagtatcacc gtgaagataa aggggaagaa caggatcagc
     3001 ggcaggaaat tgaagaaatg gaaaagtgaa cggatgttca gaaaagatac cagctgccgg
     3061 cgacggatgt tctgcgacag gtagggtgtc accgtcaaca cgggaagttc ctgcatggcc
     3121 agccggagta tgatatcgat gccgaaataa tagaggatga aactgcaaaa ggtgtcgatg
     3181 atatcctggt gcgggagcag ctgctgaagc agatgtttga gggcgaagcc caggaagagg
     3241 gcgcaaccaa ggaggtaaag gatcatgaac ccgaacaaca gctgaagtgc cagattcctg
     3301 ccggcgcccc ggcgtcgcca gaacgatttc cattgatggg aaagcagtgt gaggatcata
     3361 gacgaggatc gtttaatgcc gtcaaattag ctattattcc agggcggcgg gaattcttta
     3421 cataaaaaag ccgattgtaa cgacgaaccg gccgtcattt tgcctaattt tattgacgga
     3481 gattataatc gttatatcta atgtatgatg gcaaggatcg gccggatcat cgtccggaca
     3541 attttagcgt tattgatctt ttttattact cttcttctcg tttttgaccg gctggtgcag
     3601 ttcaggatgg acaatagcga attattgacc tggttcagcg gccgtcatat ccaaccgaag
     3661 atcggatatt ataaaacgga gggccgccag atacgctatg tgcaggtggg cgattctccg
     3721 gatgctacca tcctgttcat ccatggcgcg cccagctctc tcagctattg gaagagttac
     3781 ctttccgaca gcacgttgct gtcgcgggcc acgatgttcg cggcggaccg gccgggctat
     3841 ggatattccg ggttggccga cccattgccg gatatagccc aacaagcagc cgcgcaagtc
     3901 cctatgctgg atagtcttca caaggctcat catccgatca tcgtggtagg agtttcttat
     3961 ggcagcccta tcgcctgccg gttggcgatg gatcatccgg aactggtgga tggactcgtg
     4021 ctggtggcgc ccccgctggg cccggggcag gaaaggatgt tctggttcac ctatgctatc
     4081 gagagcccgc tgattcactg ggtggtgccg cgtatgctgc aaacggccaa ccaggaaaag
     4141 gtccatcaca aagaggagct gacgaagatg ttaccgctgt ggggacggat ccatgtaccc
     4201 gtaatctatc ttcagggtga aaaggatgga ctggtggata ccacgaatgc cactttcgcg
     4261 cgcgagcatt taacgaatgt cccctacctg gatatacgaa tgataccggg caggggacac
     4321 ctgattgctt tcgcggaaaa ggataggatc gaaaaagcga tattgcagat tctcgacaag
     4381 accgaaaata ggaagtaggg aatattttaa ttcgtcgcaa gatgactcct gaagcccagg
     4441 atctccatcc tttcgggctc gacctggcgt tcgtgctggc ctgctatctc cgcgactgtc
     4501 atcggacttg tgatgatcgt atcgaatccg aggtggttgc tgagtcgctg tgattgcgcg
     4561 acatccagtt ttccgaactc gtatttggcg atcagtcgtc ctttccggag cagcgcgctg
     4621 tcgaccaggg tcaaagggct gttgaaagtg cagatcagct ggacgctcag aaaatccgaa
     4681 agcagtccat ccgaaatgtt caacaggttc gagaccgatg agcccggatt gtgtcttcgg
     4741 tccatgatca cattctccgc atcctcgatg atgagcaccg tatccggatt gcttgtcagc
     4801 aactggataa agtccgggtt catgagatcg ttggtgactg ccggtgaaac gaacaggatc
     4861 cgcttcttta tccggctgat cagatagcgc agataggtcg tcttacctgt ccccgggatc
     4921 ccatgcaaca gcacgatccc tttatccttc gacttgttca atcgttgccg gatgaccgta
     4981 tcgatctccc gaaaaccagg attgtagtaa aggccaaggt ccagccgtgt ccgtttgatc
     5041 tccatcgcct ttagctccag ccggttgttt cccctgatga ccacattgat ctcgagcgcc
     5101 tcgcgacgct gccgttgttt gaacggtgcg atcaggtttg ccgcttctat caggaatgcc
     5161 gcttcccggt cattatgcca gagctcacag acggactgat cgaattctgc aacgcattga
     5221 ttatccagga ctgccaacgc ccgccagaaa cagaccttct ttttctcctt gtcataccat
     5281 tcgtatttaa atacccgggc tatcctggcg gcatattttt ccgaaaaggc tgtgaaggct
     5341 ttttcaccgt cgatcccgct gatatagaac tcgcttggca agtcattaaa ataatggcag
     5401 tatagcgctc tcgcgtcgac cgggtcttca ttgaaaaggt cgatggtgtt tactatcatg
     5461 c
//
LOCUS       DASYSV010000298        11012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1080415, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000298 DASYSV010000000
VERSION     DASYSV010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11012
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080415"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1137)
                     /gene="glk"
                     /locus_tag="VGQ51_08930"
     CDS             complement(124..1137)
                     /gene="glk"
                     /locus_tag="VGQ51_08930"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEV9036732.1"
                     /translation="MIPIAFKQKRAQEGMNILAADIGGTKTNIALYHAEGGELHIVNE
                     KKYWSKDHSTLSDIIHDFTGDQLPDRISAAVAGPVVKGKSRLTNLPWVLDSAVMSKEW
                     KVPVHFINDLEATAYGLAGLGKDELAVLAAGDPAAVGNIAIIAPGTGLGEAGLYWDGE
                     EYHPFATEGGHSDFAPRTREDVELFYILQEQFGHVSWERLVSGMGIENIFHCLVRHRG
                     EAVPEWLAQRMLDEDPAAVISEAALKHEDLLCAETMDLFVRYLATEASSLVLKLMATG
                     GLYIGGGIPPKILPLLQTPAWKKNFDNNGRMHELSAKVPVRVILNDKMALQGAAYYGA
                     YSL"
     gene            complement(1146..4481)
                     /locus_tag="VGQ51_08935"
     CDS             complement(1146..4481)
                     /locus_tag="VGQ51_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV9036733.1"
                     /translation="MSYTPIHKIRLVTAAALFDGHDAAINIMRRILQSKGAEIIHLGH
                     NRSVAEIVECAIEEDVQGIAITSYQGGHIEFFKYMKDLLEENGCGHIRLFGGGGGTIL
                     PQEIEELHRYGITRIYSPDDGRKMGLEGMIEDVIRQCDFALNGNADQFRGKMTLGEWK
                     DVRKIARAITNAENGVAAAGSVTAQGPVAAVAGTIPVVGITGTGGAGKSSVTDELVRR
                     FLHSFSDKTIAVVSVDPSKKKTGGALLGDRIRMNAITGGRCYMRSLATRDSDKALSAH
                     VQEAIDICKSAGYDLVIVESAGVGQSDASILEHAGISIYVMTPEYGAASQLEKINMLD
                     YADLIVLNKFDKAGAQDALHDVRKQYKRNHGLWAAKDGDLPVIGTIASQFNDAGVNEL
                     FEKLIVAVEKKTGIRYGELTLHAHTADTTTQSKILPPSRVRYLSDISGAIREYNRRTE
                     EQAVIASRLYQLNGVLEHLPAGSAARPELVAQKEKLELQLYPLCKKLIDEWPAIVMRY
                     RQDSLDYDVRGKVISQPLYTESLSGTAIPRIAVPRIRDWGDILRWRLQENFPGKYPYT
                     AGVFPLKRTEEDPTRMFAGEGGPERTNKRFHYVSFGQPAKRLSTAFDSVTLYGEDPDH
                     RPDIYGKIGNAGVSIATVDDAKKLYSGFDLCDPRTSVSMTINGPAPILLAFFMNAAID
                     QQCEKYISENNITVPKRPDHPVYTGELPEGNDGLGLGLLGLSGDQVLAADVYEKIKAS
                     TLSQVRGTVQADILKEDQAQNTCIFSTEFALRLMGDVQSYFIRRVVRNFYSVSISGYH
                     IAEAGANPITQLAFTLANGFTYVEYYLSRGMSIDDFAPNLSFFFSNGMDPEYGVIGRV
                     ARRIWAKAIKHVYKGNGRSQKLKYHIQTSGRSLHAQEIDFNDIRTTLQALYAIYDNCN
                     SLHTNAYDEAITTPTEESVRRAMAIQLIINRELGSTKTENYIQGSFAVEELTDLVEKA
                     VMDEFDRLTDRGGVLGAMERMYQRNKIQEESLHYESMKHSGELPIVGVNTFLDRKGSP
                     TILPGEVIRSTKEEKDRQIANLLAFRGRNAGRSAEMLQRLKTVATGEGNLFEELMETV
                     KYCTLGEITGALYEVGGQYRRNM"
     gene            4648..6084
                     /locus_tag="VGQ51_08940"
     CDS             4648..6084
                     /locus_tag="VGQ51_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEV9036734.1"
                     /translation="MTHRNSTLCLILVAAYLALPFCSSAQTVLPPNQPEQDACHALTL
                     CGGKFFTPYSYSGTGHNLDLNSTPCGSGEDNSMWLKVVIATAGDLVFRIIPKDTLDDY
                     DFAVLDATSANCSSLTQSDVVRCNFNNNWPGSNPLGIVGLSDSSNIRFVPDGYTGNSF
                     NQSIPATAGHTYLIMINNFGHDSTPGPSNGFTIDFSGSTATFASDSYPAFESIVKQCS
                     DSSLTVRMTKPIICSSIAADGSEFTITPFVPIAGAIGINCVNTNGYTSQVSITLGTHI
                     APGQYVLHAQKGAGGMIIQDLCGYPMTLSVAIPFTVPVPIQGKFLPPDTTKCNYSTIS
                     VIPARTFATYAWSNGQNTPTIAVTDPGVYTLHVADSNTCLGVDSISIRDSTCPQYVYI
                     PTAFTPNGDGRNDKFKVTFAGPTNEFRMAVYDRWGRMVFETNDPFSGWDGTTSGNQQP
                     TGTYAYICIYRLYQQSQRIQRGTVLLIR"
     gene            6178..6744
                     /locus_tag="VGQ51_08945"
     CDS             6178..6744
                     /locus_tag="VGQ51_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036735.1"
                     /translation="MRKLAAILLMGMLFFNWYGYQLLSAYWEQRADNRLEASLDRNEY
                     NEAQLVSIKIPVTDLSYYNSSTTFERVDGTIDVGGVQYKYVKRRIFKDSLELLCIPNM
                     TAMQLRQSKNDFFRQVNDLQQQTNQGKKHNTTTNDISKDYTPTAMDITVPALTAASMP
                     ALSIFGSPDLPSRYVPTAEMPPDATGLA"
     gene            6824..8725
                     /locus_tag="VGQ51_08950"
     CDS             6824..8725
                     /locus_tag="VGQ51_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036736.1"
                     /translation="MKDLTGEGLNGPAQRIYHTLRIASAMCFFGHGAFGIITKSVWCN
                     YFGVFGIGHDLAYRLMPFVGTVDILMGISLLVYPVRAIFGWLVCWGFLTASLRPLSGE
                     PVGELLERAGNFGAPLALLLLTGPIGSPKSWWSRIDPHFKPDARHYARVVVCLRIVVF
                     LLLLGHGWLNLIEKKSLLNQYAILGFDNPAGTAHIIGILEMMAALSVILRPARWVLFT
                     LFIWKMATETFYPHWEVFEWIERGGSYGSILALWFALGGLDIKIFTTTKTNSMRKVFV
                     SFLFFAALAFANRVSAQGCVAIRSNGGFCTAGEEQGHVDTSAQWQLSINNRYYKSFRH
                     YVGTAYQKQRQVLGNEVINNGYTMDLAIYRILNPRWTLMLDLPISANARSQTYQQKGV
                     YNRFSTHAFGAGDIRVAVYRWLLDPVKMPKGNIQVGLGLKFATGNDNVQDYFKTSDST
                     KTFGPVDQSIQLGDGGTGISFELNGYYNFSHRVGLYGNFFYLSNPADQNGISNAHGGT
                     PSASSIANGSNVMSVPDQLMARAGVSMQFNRLNVSAGMRDDCLPVYDLIGKSDGFRRP
                     GYIISAEPGLTYVFNKISLYTYVPIALVRNRTQSEPDKISTQLTGKYTQGDAAFANCV
                     VNIGANIRF"
     gene            8747..9706
                     /locus_tag="VGQ51_08955"
     CDS             8747..9706
                     /locus_tag="VGQ51_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036737.1"
                     /translation="MMLIKRIGVRLAALSLLLWPALLRAQTDQDAIMMSKNLLCVGAS
                     YGYSSWNEYWEGTFHRNNPNLGTASTQMAGLMGIYGVTRKFNVEVNVPYIWTHSTAGT
                     LHGQKGFQDLSVWLKWKTLEQNVNSPVSVFVLGGASVPMTNYIADYLPLAIGMQSKTL
                     SGRLILDYQHRHFFVTASGTYTWRGDITIDRDAYYTTSLHLTNQVDMPNMMSEQLRTG
                     YRNGDRWVAEAVVSNMNTLGGFDITRNNMPFPSNKMNETMIGAHVKHGFKAVDGLSLD
                     VDGAYTVAGRNMGQSTMFDVGVYYIFHFSKKASKHSSNQSSTN"
     gene            9709..>11012
                     /locus_tag="VGQ51_08960"
     CDS             9709..>11012
                     /locus_tag="VGQ51_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA-phosphatase"
                     /protein_id="HEV9036738.1"
                     /translation="MQRLFYLLSMIALVGAAGCNKNVADRTANLQALNPANEDLNAGA
                     WKPVLLPRPDTFAVAAPAATTNPSYIADLNEIKGLQAHLTADGQTQIKYWSAGGVLRW
                     NEIMLGLMAKYNLPPYQNADGTYPAPNSANPFAYPLFPFSNPPYAARALAYISAAQYD
                     ALVACWHYKALYNRPAPCLVDSGVKALAGRRNLPSYPSEGAVLAGVTAELMQLLFPDE
                     VASIQQKLEQQELSLIASGAAARSDVTAGEALGRQVADLYLARGRADNAGKAGGNAAN
                     WASYVTTAQANGQTPWISLETPGRPPMLPLFGRVKGFLCDSAAIISLRPGPPPSTSSA
                     DMTAAVQAAYTTIKNATRDQMRIVQYWADGVGTFTPPGHWNAIADADFINQNYSEVRW
                     ARNLALLNMAELDAAISCWDIKYFYFNPRPTQMNANIKTLTG"
BASE COUNT         2420 a         3254 c         2796 g         2542 t
ORIGIN      
        1 cctgtagcta attacgacca cacactatga ttatggataa agatagagga aaaatttcaa
       61 taattgaaat attatttcaa aatttaaaaa aatgacccaa caatagtcat ttactcaatt
      121 ttttcacaaa ctatacgccc cataatacgc cgcaccctgt agcgccatct tatcattcaa
      181 tatcaccctc accggcacct tggccgacag ctcatgcatc cgcccgttat tatcgaaatt
      241 cttcttccat gccggtgtct gcaacaacgg aaggatcttc ggcgggatgc cgccgccgat
      301 atacagtcct ccggtagcca tcagcttcag cacaaggctc gaagcctcgg tcgcgagata
      361 gcgaacgaag aggtccatcg tctcggcgca aagaaggtct tcgtgtttga gcgccgcctc
      421 actgatgacg gcggcggggt cttcatccag cattcgctgc gccagccatt cggggacggc
      481 ctcgccacga tgacggacga gacaatgaaa gatattttcg atgcccatcc cggagacgag
      541 acgctcccag ctgacatgtc cgaattgttc ctgcaggata tagaacagct ccacgtcctc
      601 acgcgtccgg ggagcgaaat cgctatggcc gccttccgtc gcaaacggat gatactcctc
      661 accgtcccaa tacaatcccg cctccccaag cccggtaccc ggcgcgatga tcgcgatatt
      721 gccgacggcc gcggggtcgc cggcagccag aacggccagc tcgtcctttc cgagaccggc
      781 cagaccatag gccgtcgctt cgagatcatt gatgaagtga acgggaacct tccactcctt
      841 gctcatgacg gcgctgtcca gcacccatgg cagattggtg agacgactct ttcccttgac
      901 gacaggaccg gcgacggcgg cggagatccg gtcgggtaat tgatcgccgg taaaatcatg
      961 gatgatgtcg gatagcgtgg aatggtcttt ggaccaatat ttcttctcgt tgacgatatg
     1021 cagctctccg ccctcggcgt ggtaaagcgc aatattggtc ttggtgccgc cgatgtccgc
     1081 ggcaagtata ttcatgcctt cctgcgcgcg tttctgctta aatgcaatgg ggatcatccc
     1141 caaaattaca tgttcctccg gtattgtccg ccgacttcat acaacgcgcc ggtgatctcg
     1201 cctagcgtgc agtatttcac cgtctccatc agctcttcga agagattgcc ttcgccggta
     1261 gccaccgtct tcagacgttg caacatctcc gcactgcgac cggcattgcg acctcgaaag
     1321 gccagcaggt tcgcgatttg ccggtctttt tcttcttttg tgctgcgtat cacctccccg
     1381 ggcagaatag taggcgaacc ctttcggtcg aggaaagtat tgacgccgac gatcggcagc
     1441 tcaccgctgt gtttcatgct ctcgtaatgc agactctcct cctgtatctt gttgcgctga
     1501 tacatccttt ccatggcgcc gagcactcca ccccgatcgg tcagccggtc gaattcatcc
     1561 atcaccgctt tctccaccag gtcggtcagc tcttccacgg cgaagctgcc ctggatatag
     1621 ttctccgttt tggtgcttcc cagctcacgg ttgatgatca gctgaatggc cattgcccgt
     1681 cggacgcttt cttctgtcgg ggtcgtgatc gcttcgtcat aggcgtttgt gtgcagcgag
     1741 ttgcagttgt cataaatggc atacaacgcc tgcagcgtcg tccggatgtc gttgaagtcg
     1801 atctcctgcg catgcaggct cctgccgctg gtctggatat gatatttcag tttttgtgaa
     1861 cggccattgc ctttataaac atgcttgatc gctttggccc agatgcgccg cgcgacgcgg
     1921 ccgatgacgc cgtattccgg atccatgccg ttgctgaaaa agaaggagag attgggagcg
     1981 aaatcgtcga tcgacatccc cctgctaaga taatattcca cataggtgaa cccgttggcc
     2041 agcgtaaagg ccagctgcgt tatagggtta gccccggctt cggcaatatg atatccgctg
     2101 atggacacac tgtaaaagtt gcgaacgacg cgtctgatga aataagactg aacgtcaccc
     2161 atcagacgaa gggcgaattc ggtactgaag atgcaggtat tctgtgcctg gtcttctttc
     2221 aggatgtcgg cctgaaccgt gccacgtacc tgcgacaagg tggatgcttt gatcttctca
     2281 tagacgtcgg ccgcaaggac ctggtcgccg ctgagaccca acagacccag acccaggccg
     2341 tcgttgcctt cggggagctc cccggtatac actggatgat ccggtcgttt gggaacggtg
     2401 atgttattct ccgagatgta tttttcgcat tgctgatcga tcgccgcatt catgaaaaag
     2461 gccagcagga tgggtgccgg cccattgatg gtcatactta cggaagtccg cggatcgcaa
     2521 agatcgaatc cgctgtacag cttcttggca tcgtccaccg tcgcgatgct cacgccggca
     2581 ttgccgatct tgccatagat gtcgggtcgg tgatccggat cctcgccata taaggtcacc
     2641 gagtcaaagg cggtactcaa tcttttggcc ggctgaccga atgaaacata atggaaacgt
     2701 ttgttagtac gctcggggcc accttcaccc gcaaacattc gcgtcgggtc ctcctcggtt
     2761 cgtttcaacg gaaagacgcc ggccgtataa ggatatttcc ccgggaaatt ctcctgcagc
     2821 cgccaccgga ggatatcccc ccagtcacga atacgcggca cggcaattcg aggtatcgca
     2881 gtcccggaaa gcgattcggt atacaatggc tggctgatca ccttcccccg gacatcgtag
     2941 tccagcgaat cctgccggta acgcatcacg atagccggcc attcgtcgat cagcttttta
     3001 cagagcggat acagctgaag ttccagcttt tctttttggg cgaccagctc aggccgggca
     3061 gcggatccgg cgggtaggtg ttcgagaacc ccgttcagct gataaagccg cgaggcgatg
     3121 accgcttgtt cttccgtccg gcggttgtac tcccggatgg ctccgctgat atcggacaga
     3181 tagcgtaccc gggaaggtgg caatattttg gattgtgtgg tcgtgtcggc ggtatgggca
     3241 tgcagcgtca gctcgccata gcgtattccg gtcttttttt caacggcaac tatcagcttc
     3301 tcgaaaagct cgttcacccc tgcgtcattg aactgcgagg cgatggtgcc gatgacgggc
     3361 agatccccgt cctttgccgc ccataatcca tgattccgtt tgtattgttt gcggacatca
     3421 tgcagtgcgt cctgtgcccc cgccttatcg aatttgttga gcacgatgag atcggcatag
     3481 tccagcatgt ttatcttctc gagctgtgac gcggccccat attcgggcgt catcacatat
     3541 atgctgatac cggcatgctc caggatggaa gcatcgcttt gtcctacccc cgccgattcg
     3601 acgatcacga ggtcatagcc tgcggacttg cagatgtcga tggcttcctg tacatgggcg
     3661 ctcagggcct tgtcgctgtc ccttgtcgcg agactgcgca tatagcagcg gcctccggtt
     3721 atcgcattca tgcgaatgcg atcgcctaaa agagcgccac ccgttttttt cttggaagga
     3781 tccaccgaaa ccaccgcgat agtcttgtcg gaaaagctgt ggagaaaacg ccgcaccagc
     3841 tcatccgtca ctgaagattt acctgcgccc cccgtcccgg taatacccac gacgggtatc
     3901 gtgcccgcaa ccgccgcgac aggaccctgg gctgtcacac taccggccgc ggccacgcca
     3961 ttttccgcat tggtgatcgc cctggcgatc ttccttacgt ccttccattc ccccaacgtc
     4021 atcttccccc taaactgatc cgcattcccg ttaagcgcaa aatcgcattg ccggatcacg
     4081 tcttcgatca tcccctccag acccattttc cggccgtcgt ccggggaata gatacgggtg
     4141 atcccatacc ggtgcaactc ttctatctcc tgcggcagga tcgtgccgcc acccccgcca
     4201 aaaagacgaa tatggccgca accgttctct tccagcaggt ccttcatata tttgaagaat
     4261 tcgatatgcc ctccctggta tgaggtgatc gctattccct ggacatcttc ctcgatggcg
     4321 cattccacga tctcggccac ggagcggtta tgacccagat gaatgatctc cgcacccttc
     4381 gattgcagta tccggcgcat gatattaatg gcggcgtcat gtccgtcaaa aagggccgcc
     4441 gcagtcacta gccggatttt atggatagga gtataggaca tttgtaaaga tttggaagac
     4501 aagcaaattt agacaaaaaa actaatgttc gttagtttcc tggccttgtt tctggcagtg
     4561 tttttgacct attgccacct gcgtagggaa gcgtgacgcc agcaacccta acttgttaag
     4621 gatcgtaata ttaggtgtca cagcactatg acccatcgga actccaccct ctgtctcata
     4681 ttggtagcgg cttacctggc gttacccttt tgttcgtctg cccaaacggt gctgcccccg
     4741 aaccagccgg aacaggatgc ctgtcatgca cttaccctat gcggcggtaa attctttact
     4801 ccatacagtt attccggcac gggacataat cttgacctga actctacccc ttgcggctcc
     4861 ggggaagaca attcgatgtg gctcaaggtc gtcatcgcta ccgcgggcga cctggtcttc
     4921 cgtatcatcc ccaaggatac cctcgacgac tatgattttg cggtgctgga tgctaccagt
     4981 gccaactgca gcagcctgac ccaatccgac gtcgtccgct gcaatttcaa caataactgg
     5041 ccgggaagca atccgctggg tatcgtcggg ctcagcgatt ccagcaatat ccggttcgtt
     5101 cccgacggct ataccggcaa ttcctttaac cagtccatcc cggcgacggc gggtcatacc
     5161 tatcttatca tgatcaataa tttcggtcat gactccacac ccggccccag caacgggttt
     5221 actatcgatt tttccggatc caccgccacc ttcgccagcg attcataccc ggccttcgaa
     5281 agcatcgtca aacaatgcag cgacagctcg ctcactgtcc ggatgaccaa gccgatcatc
     5341 tgcagctcga tcgccgcgga cggcagcgag tttacgatca cgcccttcgt accgatcgca
     5401 ggcgccatcg gtatcaactg cgtcaacacc aacggctata cgtcgcaggt gagcatcaca
     5461 ctgggcacac atatcgcgcc gggtcaatat gtccttcatg cgcagaaagg agcgggcggt
     5521 atgatcatcc aggacctctg cggctacccg atgacgctgt cggttgcgat cccttttacc
     5581 gtcccggttc cgatacaagg caaattcctg ccgcccgaca cgaccaaatg caattatagc
     5641 acgatatcgg tcattcctgc caggacgttt gccacctatg cctggagcaa cggccaaaac
     5701 acgccaacta tcgccgtgac ggacccgggc gtatatacgt tgcatgttgc ggatagcaat
     5761 acctgtctgg gcgtcgattc catctctatc cgtgattcca cctgtccgca atatgtctat
     5821 atccccacag cctttactcc caacggcgac ggaaggaacg ataagttcaa agtcaccttt
     5881 gcaggtccga ccaacgaatt caggatggcg gtatacgacc gatgggggag aatggtcttc
     5941 gagaccaatg atcccttttc cggctgggac gggactacaa gcggcaatca gcagcccacc
     6001 ggtacctatg catatatctg tatctaccgg ctttatcagc agtcgcaacg gatacagcgt
     6061 ggcaccgtgc tgctcatccg gtgatccggc cgcccttgcc tgccttcgtc cgaaccgctt
     6121 ctccagaatt tctccaaaaa aaccccgcac atcaaaaaaa tgtatatctt gtgattcttg
     6181 cgaaaacttg ctgccatact gctcatggga atgctgtttt tcaactggta cggttaccag
     6241 ttactgagcg cctattggga gcagcgtgcg gacaacaggc tggaggcctc tctcgaccgc
     6301 aatgaataca atgaggcgca attggttagc atcaagatcc ccgtcaccga tctttcttat
     6361 tacaatagca gtaccacgtt cgaacgtgtc gatggcacca tcgatgtcgg cggtgttcaa
     6421 tacaaatatg tcaaacgccg tattttcaag gactcgcttg agctgctttg tattcccaac
     6481 atgacggcca tgcagctccg ccagtcgaag aacgatttct tccgccaggt gaacgatctg
     6541 cagcagcaga ccaatcaggg caagaagcac aataccacga ccaacgacat ttccaaagat
     6601 tatacgccga cggcgatgga catcactgtc ccggcactca cagccgcatc gatgccagcc
     6661 ctctctatct tcggctcacc cgatctcccc tcacgctatg tccctacggc ggaaatgccg
     6721 cccgatgcta caggcttagc ctaaactcaa gacaggtgtt cccgcgaata ccggatggcg
     6781 ccgatcagcg atcgcgccgc agaatcagca tcaaagcgtt ttcatgaaag atcttacagg
     6841 tgaagggctc aacgggcccg cccaaagaat ataccatacc ctgcgtatcg catcggccat
     6901 gtgctttttc ggtcatggcg cattcggcat cattaccaaa tccgtctggt gcaactattt
     6961 cggcgtgttc ggtatcggtc atgacctcgc ctaccggctc atgccattcg tcggtaccgt
     7021 cgatatcctc atgggtattt cgctactggt ctatccggtc cgcgccatat tcggctggct
     7081 ggtatgctgg ggatttctta cggcctctct tcggccgctg tcgggcgaac ccgtcggcga
     7141 gctgctcgaa agggcgggta atttcggtgc gccgcttgcc cttttgctgc tgaccggccc
     7201 gataggatca ccgaaaagct ggtggtcgcg catcgatccg cactttaaac ccgatgcccg
     7261 ccactacgct cgtgttgtcg tttgtctgcg gatcgtcgtt ttcctcttat tgcttggtca
     7321 tggctggctc aacctcatcg aaaagaaaag cttgttaaac caatatgcta tcctgggttt
     7381 cgataacccg gccggaacgg cgcatatcat aggcattctc gaaatgatgg cggcactgtc
     7441 ggtcattctt cgtccggcac gctgggtttt gttcacactc tttatctgga agatggctac
     7501 cgaaaccttc tatccccact gggaagtgtt cgaatggatc gaacgcggcg gcagctatgg
     7561 atcgattctg gctttatggt tcgccctcgg cggactcgat ataaaaattt tcaccaccac
     7621 caaaactaat agtatgagaa aggtattcgt cagcttttta ttttttgcgg ctctggcatt
     7681 cgccaaccgg gtttcggcac agggatgtgt cgccatccgc agcaacggcg gattttgtac
     7741 cgccggcgaa gagcagggac atgtcgacac gtccgcacaa tggcagctca gcatcaacaa
     7801 ccgctattat aagtccttca gacattatgt gggcacggcg tatcagaaac agcgccaggt
     7861 gctgggcaac gaggtcatca acaatggcta tacgatggac ctcgcgatct accgcattct
     7921 gaatccccgc tggacgctca tgctggacct tcccatttca gccaatgcgc ggtcccagac
     7981 ttaccagcaa aagggtgtct acaaccgatt tagcacacat gccttcggcg ccggcgacat
     8041 ccgggtcgcg gtctaccgct ggctgctcga tcccgttaaa atgccaaaag gcaatataca
     8101 ggtggggctg ggactaaaat tcgcgaccgg taatgacaat gttcaggact atttcaagac
     8161 cagtgacagc acgaagacct tcggacccgt tgatcaatcc attcaattgg gcgacggagg
     8221 caccggcatc tccttcgagc tgaacggtta ctataatttt tcacatcgcg ttggtctcta
     8281 cggtaatttc ttctatctgt ccaaccctgc cgatcagaac ggcatttcca acgcccatgg
     8341 cggaacgcct tccgcctctt ccatcgccaa tggaagcaat gtcatgagcg tccccgatca
     8401 gctgatggcc agggccggcg tgagcatgca gttcaaccga ctcaatgtat cggccggtat
     8461 gcgcgacgac tgcctgccgg tatatgatct catcggcaaa agcgatggtt tccgccggcc
     8521 gggttacatt atctccgccg agccgggatt gacctatgtt ttcaataaaa tatcattata
     8581 cacctatgtg cccatcgccc tggtccgcaa tcggactcaa agcgagccgg ataagatctc
     8641 gacccagctg accggcaaat acacccaggg cgacgcggca tttgccaact gtgtcgtcaa
     8701 catcggggcg aacatccgtt tctaagaacc tacaatgcca aaaccaatga tgcttattaa
     8761 aagaataggc gtccgtcttg ccgctttgtc gctgctgcta tggcctgccc ttcttcgggc
     8821 ccagacggac caggacgcca tcatgatgtc gaaaaatctc ttatgcgtag gagcctccta
     8881 cggatacagc agctggaatg agtactggga gggcaccttc catcgtaaca atccgaacct
     8941 gggtaccgcg tcgacccaga tggccggtct gatgggtatc tacggcgtga cgcgcaaatt
     9001 caatgttgag gtcaacgttc cctatatctg gacgcattcg accgccggca ccctgcatgg
     9061 ccaaaaagga tttcaggacc tctccgtgtg gctgaaatgg aagacgctgg agcaaaatgt
     9121 aaatagtccc gtttcggtgt tcgtacttgg cggcgcctcg gtccctatga ccaattatat
     9181 tgcggactac ctgccgctcg ccatcggtat gcaaagcaag accctgtccg gcagactgat
     9241 ccttgattac cagcacagac atttcttcgt caccgcctcg ggcacctaca cctggcgcgg
     9301 cgacatcacc atcgaccggg atgcctatta tacgacctcg ctgcatctga ccaaccaggt
     9361 cgatatgccg aacatgatgt ccgaacagct ccgcaccggg tatcgtaatg gcgaccgttg
     9421 ggtagccgaa gccgtcgtct cgaatatgaa tacgctcggc ggtttcgaca taaccaggaa
     9481 caatatgccg ttccccagca ataagatgaa cgaaaccatg atcggagcgc atgtcaaaca
     9541 tgggttcaaa gccgtggacg ggctttcgct cgacgttgac ggcgcatata ccgtcgcggg
     9601 ccgtaatatg ggacaatcca cgatgttcga cgtcggggtc tactatattt tccatttttc
     9661 gaagaaagcg tcgaagcatt cctctaacca atcctcaacg aattaattat gcaacgtctt
     9721 ttctatcttc tttcgatgat cgctttggtg ggtgctgcgg ggtgcaacaa aaacgttgcc
     9781 gaccgtacgg ctaatctgca ggcactgaac cctgccaatg aagacctcaa cgccggcgcc
     9841 tggaagcccg ttctgcttcc ccggcccgat acttttgccg tcgcagcgcc ggcggccacc
     9901 accaatccgt cgtacatcgc agatctcaat gagataaaag gccttcaggc ccacctaacc
     9961 gccgacgggc agacgcagat aaaatactgg agcgccgggg gcgtgctgcg ctggaatgag
    10021 atcatgctcg gtctaatggc taaatacaac ctgccgccat accagaatgc cgacgggacc
    10081 taccccgccc ccaacagtgc gaaccctttc gcttatccgc tgttcccttt ttcgaatcct
    10141 ccttatgcgg caagagccct ggcctatatc agcgccgccc aatacgatgc actggtggct
    10201 tgctggcatt acaaggcact gtataaccgt cctgctccct gcctcgtgga ctccggcgtg
    10261 aaggcgctgg ccggaagacg caatctgccg tcctatccgt ccgaaggcgc cgtccttgcg
    10321 ggcgtcacgg ccgaattgat gcagctgttg ttccccgatg aagttgcgag tatccagcaa
    10381 aagctggagc agcaggagtt gagcctcatc gcgtccggag cggcggcccg cagcgatgtg
    10441 accgccggtg aggcgctggg tcgccaggtg gccgaccttt acctggcgag gggtagggcc
    10501 gacaatgccg gtaaggccgg cggcaatgcc gccaactggg ccagttatgt caccacggcg
    10561 caagccaacg gccagacccc ctggataagc ctcgaaactc cggggagacc gccgatgctg
    10621 ccgctcttcg gaagggtgaa agggtttctt tgcgattccg cggcgatcat atccttgcgc
    10681 cccgggccgc ctccttccac cagcagcgcc gatatgacgg ccgcagtaca ggccgcgtac
    10741 acgacaatca agaatgccac ccgcgatcag atgcgcatcg ttcagtattg ggccgacggc
    10801 gtgggcacgt tcacgccgcc gggtcattgg aatgccatcg cggacgccga ctttataaac
    10861 cagaattata gcgaggtgag gtgggcacgg aacctggcgc tgctcaacat ggcggaattg
    10921 gatgccgcca taagctgctg ggatatcaaa tacttttatt tcaacccccg gcccacccag
    10981 atgaacgcca atatcaagac actgacgggt at
//
LOCUS       DASYSV010000299         4054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_314135, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000299 DASYSV010000000
VERSION     DASYSV010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314135"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VGQ51_08965"
     CDS             <1..1029
                     /locus_tag="VGQ51_08965"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEV9036739.1"
                     /translation="DLIVVGEWMPVTFFHNDHGKFTNVTGKTGIGDKTGWWNSIAAGD
                     FRHTGRTDYIIGNLGLNSLYRASDRYPVCVTAGDFGNSGTYIAIPSIYLPDVKGEKKE
                     FPAMGRDDIARQMPGIKKKYPTYKPFALATMDDILSPEQRKSAIRLSANEMRSVYLRN
                     DGNGRFTMIPLPIGAQVSVINGMVVDDFDGDGNPDVLLNGNDYGTEVGTGRYDALNGL
                     LLKGDGKGHFTPQSIAASGIYIPGNGKALVKLQGARGQYLIAASQHRGPLKLYRLNKA
                     PLVIRMTPDITYAIVHFADGHIQKTEAYDGSSFLSQSGRFLTAGKNASYVELFNSKGL
                     AAKIKVPQ"
     gene            complement(2235..4022)
                     /locus_tag="VGQ51_08970"
     CDS             complement(2235..4022)
                     /locus_tag="VGQ51_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV9036740.1"
                     /translation="MRKWLVASMIAGLIGTGVHGQPPSGHPPAGQSPHEKYSVDAASE
                     EHAGVPKGELLHFTLNKSNIFPGTRREYWVYVPAQYRPDKPACVYVNQDGVQWKAPTV
                     FDNLINSGEMPVTVGIFVQPGRVIAQDTAAELDRFNRSFEYDGIGDGYSRMLLDELLP
                     EVEKQHTSDGRPIRLSPNGNDRAIGGSSSGAVCAFTVAWQRPDAFTRVFSAIGTYVGL
                     RGADRYPILIRKYEPKPIRVFMQDGSNDLNIYGGDWWMANQTMERALIFAGYEVRHVW
                     GEGGHSGAQGTSLFPEAMRWLWKDWPRPVAKGSSANQVLHDILASGEDWELVGEGYGF
                     TEGTAADATGNVFFQDIPASATYEVKPGGHPVVISKEAKKASGTCFTTDGSRYEVATG
                     SRQILRYEDGGKVSVIADNIAGNDLLVARNGNVYVTAPDGTEKPSKLYLIRPGRQPVV
                     VDSGLKFINGLCMTPDQSQLYVAESASHWIWIYSILSDGSLGNKQRFGWLHSPDDQET
                     AWPDGMKCDREGRLYVATRLGIQVLDPLGRVNAILPVPSGQASNCCFGGRDFDVLYAS
                     CGGRVYRRRLRVRGCNPFEAPNKPGPPHL"
BASE COUNT          902 a         1276 c         1003 g          873 t
ORIGIN      
        1 gatctcatcg tagtgggcga atggatgccc gtcacctttt tccacaacga ccatggaaaa
       61 tttaccaatg tcaccgggaa aacgggcatc ggcgacaaaa caggctggtg gaattccatc
      121 gcggcaggag atttccggca cacgggtcgc actgactata tcatcggcaa tctcggtctg
      181 aactcgctct acagggccag cgaccggtac ccggtgtgcg tcaccgccgg cgatttcggc
      241 aacagcggca cctatatcgc cataccctcc atctacctgc cggacgtcaa aggagaaaag
      301 aaagagttcc ccgcgatggg acgcgacgac atcgcgcggc aaatgcctgg gatcaagaaa
      361 aaatatccca cctataaacc tttcgcgctg gccaccatgg acgatatcct ctcgcccgaa
      421 caacggaaaa gcgccatccg gctctcggcg aacgagatgc gttccgtgta tcttcgcaat
      481 gacggcaacg gacggttcac catgatcccg ctgcccatcg gggcacaggt ctcggtgatc
      541 aatggcatgg tcgtggatga tttcgacggc gatggcaatc ctgatgtgct gctcaatggc
      601 aacgactacg gcaccgaagt aggaacaggc cggtacgatg ccctcaatgg tctgcttctg
      661 aaaggcgatg ggaaaggaca tttcacgccg caatctatcg cggccagcgg catctatatt
      721 cccggcaatg gcaaggcact ggtaaaacta cagggtgccc gggggcaata tctcattgct
      781 gccagccagc atcgggggcc gctgaagctg tatcgcctga acaaagcgcc tctcgtaatt
      841 cgaatgaccc cggatatcac ctacgctatc gtccattttg ccgatgggca tatccagaaa
      901 accgaagcgt acgatggctc gtcgttctta tctcaatcgg gcagattcct gaccgcaggt
      961 aaaaatgcct cctatgtcga gctcttcaac agcaagggcc ttgcagcaaa aataaaggtc
     1021 ccccaatgag aaccgcgatc ctatgcaccg tggccgtatt cgccggcatc ctgctattct
     1081 cccgctgtct cgactccggg gcccgcgatc ccaggggtaa ggcctatgcc gaccctgcct
     1141 cctgcgcgtc ctgccatgcg tccatcgtca acgcttatgg acatacgccg catttccatt
     1201 cggccgcgct ggcctccttc aataccatcc ggggcagttt ttccagggac tccaacacct
     1261 tcgtggtaaa tgactcggtc cgcgtcgtta tggaagatcg cggaggaact ccctaccagg
     1321 tcctgtatgt ccggaacaag gaagtccggg tgcaacgttt cgatatcgtt ttcggttatg
     1381 agaagggaca gggctatctg tattggaagg acgaccagct ataccagctg cccatctctt
     1441 attttaccga tctgcatcgc tggaccacca gccccggcta tcccgctggc atgccttttt
     1501 tcgacagacc cgtactacta cggtgcttcg aatgccacac cacctatatc tccccttcct
     1561 cccgccgctc tggctccccc tccgatccca aaaatgtcaa tgcactcgac gcaaactcgc
     1621 tgatagtaaa catcgattgc cagcgatgtc atggccccgc cgccaatcac gtcgctttcc
     1681 acaccgaaaa tcccggcgaa aagatcggaa gatatatctc gtcctataag aaccttaccc
     1741 gccggcaaaa gatcgatcta tgcgctgtat gccacgcggg cagcaataat atccagctga
     1801 ggtccctgtt cgccttccgg cccggagatt cgctttcgca gtttgcggtg tcggcgcatg
     1861 tcgggtccaa ccagccggat gtccatggcg atcagctggc gctgctggct tccagtaagt
     1921 gtttccgggg cagcgaaatg gactgctcca cctgtcacga cacgcacgtc aatgatcacg
     1981 gcgactgggc cgcgtatgcc cgacgctgcc agacctgtca ctccgaaacc aatcataatt
     2041 tttgcaaaat agccgatgct tcgaatatcg gtttcttaaa actgaactgc tcccgctgtc
     2101 atatgccgtc gcagccatcc gaggtcatca gggtcaggac cggcgagacc ggcgcccata
     2161 cccccatctt catgatcaac caccgcatcg ccatttatcc cgaagagtcg cgccggattc
     2221 tcgacagttt aaactcacaa atgcggcgga ccgggcttat tcggggcttc aaaaggattg
     2281 caacccctca ctcgcagccg tcgtctgtaa acgcggcctc cgcaactcgc ataaagaaca
     2341 tcaaaatccc tgccgccgaa acagcaattg gaagcctgcc cggatggcac cggtaatatc
     2401 gcgttgaccc ttcccaaagg atcgagcacc tgtatcccca accgggtcgc tacatataac
     2461 ctgccttcgc gatcgcattt catcccgtcg ggccatgccg tctcctggtc atcgggggaa
     2521 tgcagccagc caaagcgctg tttatttccc agactgccat cggaaaggat gctgtagatc
     2581 caaatccagt gagaggccga ttcggccaca tacaattgcg actgatcagg tgtcatacaa
     2641 agtccattga tgaacttcaa ccccgaatcc acgaccaccg gctggcggcc gggccttatc
     2701 aggtacagtt tactgggctt ttccgttcca tcgggagccg tcacatatac atttccgttc
     2761 cgcgcgacga gcagatcgtt gcccgcgata ttgtccgcga tcacggagac cttacctccg
     2821 tcttcatatc gaagtatctg cctgcttcct gttgctacct catatcggct gccatcggtg
     2881 gtgaaacagg taccactcgc cttttttgcc tccttcgata tcacgacagg atgcccgccg
     2941 ggttttacct cgtaggtcgc cgaagcggga atatcctgaa aaaaaacatt gcctgtcgca
     3001 tccgctgccg tcccttccgt aaacccataa ccttctccca ccaattccca atcctcaccc
     3061 gacgccagga tatcgtgcag gacctggttt gcgctgctcc cttttgccac cggccgcggc
     3121 caatccttcc aaagccaccg catggcttcc gggaaaagcg aggtcccctg tgcgccgcta
     3181 tgacctccct cgccccaaac atgccgaacc tcatagccgg caaagatcag cgcgcgctcc
     3241 atggtctggt tcgccatcca ccagtctccc ccgtaaatgt tgagatcatt ggagccgtcc
     3301 tgcataaaca cgcgtatcgg tttgggctcg tatttccgga tgaggatagg gtaccgatcc
     3361 gcgcctcgta aacccacata ggtgccgatc gcgctgaaca cccgcgtgaa cgcatccggc
     3421 cgctgccagg ccacagtaaa tgcgcacacg gctccactgc tcgatccgcc gatagcccgg
     3481 tcatttccat tgggagaaag acggatgggc cttccgtcgc tggtatgctg cttctccact
     3541 tccggcagca attcatccaa cagcattcgg gaataaccgt ctccgatgcc atcgtattcg
     3601 aagctgcggt taaaccgatc cagctcggcc gccgtatcct gggcgatgac ccgaccgggc
     3661 tgaacaaaaa ttccaacggt cacaggcatc tcaccactat tgataagatt gtcgaatacc
     3721 gtcggagcct tccattgcac accatcctgg ttgacataga cgcaggccgg cttgtccggt
     3781 cgatattgcg ccggcacata tacccaatac tcccggcggg tgccgggaaa aatgttgctt
     3841 ttgttcagcg taaaatgaag cagctcgcct tttggtactc ctgcatgctc ctccgatgcg
     3901 gcatcgacgg aatatttttc gtggggtgat tgcccggctg ggggatgccc ggagggcggt
     3961 tgcccgtgaa cgccggtccc aatcaggccg gctatcatcg acgcgactag ccattttctc
     4021 atagtcggaa gatacacaat aatcctcgcc aaac
//
LOCUS       DASYSV010000300        12137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_58976, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000300 DASYSV010000000
VERSION     DASYSV010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12137
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58976"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VGQ51_08975"
     CDS             complement(<1..1138)
                     /locus_tag="VGQ51_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036741.1"
                     /translation="MSTTTQTALPPLNTPGVYIQEIPVLPASIVSVPTAVPVFVGYTE
                     KAMEGTTSLLGVPFMVENIIEYQQYFGLPYPETGLHATVDDSSPTQPVVIVDLDEPTR
                     SPYLMYYSLQLYFANGGAPCYILSVGLYKPQDPVILVSDFKPDGTTFCPQLEKYNDIT
                     LVVIPDAMSIAHTADPDKGASTYYALWNLAIDHCIEMGNRMAVLDVYPVNGHTLPIED
                     IKVLRGQDTNNSGISVTGPLPENFKYAAAYYPRIYTTITPSVKDPTTLLDRDDLIKVS
                     LKSAAAGSPPAKLSDLKAKSGQIYYAVKNYISNNLEMLLPAAPGVVGVYATVDNLQGV
                     WTAPANWPITAAVDLKEHISAKDQGPINVDPVTGMSVNVIRNFPG"
     gene            complement(1233..2594)
                     /locus_tag="VGQ51_08980"
     CDS             complement(1233..2594)
                     /locus_tag="VGQ51_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036742.1"
                     /translation="MRLLYNILFEVKVLHEYYLTDPDNTSVFQNAQQTDRLKWLNRRY
                     QADQPAGSDDLSFILPSYMTAQFRNQDLRLLSSLSGFQVAVRVNETVQSGTTVYTPAI
                     SMDGNFNLIILMQQGTGQFATLTNGRLSHPISSLYYFSNADLGAAKTAPVLSRPISAN
                     VPGYTYEQGELSLSGANVQLCYYNGTTPAFETVPGNGFINENDRLIVGRQFDYNFEES
                     DNVTQAQFVLKDPGGNPVRTMPITGTDPLGTVTINFDDAGNPALTPLLTLPKARATDA
                     ILYSLEVTGNGGYTKTIPLIFYDVASDLTSTWGVIQLQAKVANNSMSLLDPNGNLYMP
                     VLAAPDQTTSPFPVFEIRCKSRYTFWRYFSEDTTKILKAPTGTIATFVTGTTGVLTTL
                     FPIPGTYLPFFFSQNPAATPPVFTYLPNPAPDTLIEVDGFQLFSDIPVPESGIFPVSP
                     APT"
     gene            complement(2591..3214)
                     /locus_tag="VGQ51_08985"
     CDS             complement(2591..3214)
                     /locus_tag="VGQ51_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEV9036743.1"
                     /translation="MIDTAISLLQKELENYLAPIDPSATVVVDNVGLLETTNGDKLSN
                     SIIITVVNVEEESTLKNQPAYKNYPQLGTAAYENPPVYLNLYVLFTCNYWADKYIYAL
                     QRISYVVKFLQGKNTFSYGSASYQDASGNEFSDDPELVGLRFTMELYTLTFEQLNHLW
                     GSLGGRQIPFVMYKLRLIALTDRKILRNVPLIREIDADISQLTKFKS"
     gene            complement(3536..5908)
                     /locus_tag="VGQ51_08990"
     CDS             complement(3536..5908)
                     /locus_tag="VGQ51_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036744.1"
                     /translation="MLKNYIKTAIRNLRKQPGFTFINLAGLALGIACSLLILLWVQDE
                     RGVDAFHAKGRRLYYVYERNYMGGKLQTWYWTQGPLAEELIKEIPEVEAATPMSWSNT
                     NNFSVGGKILKEDGFAAGADYFSMFSYPLVEGNAMDALSSPDALAISRKMAIAFFGSP
                     AAAIGKAIKYENRRNFTVKAVFEDLTSAVSSPANYIMSWSGYVEDNGWAKRWGAVDPR
                     TVILLKPGADEAKVEKKISHLTDPFNTEVKDIRIELGLQRFSDYYLHNEFRDGVPKEG
                     RIAYVRLFSLVALFILVIACINFMNLTTARSVRRAKEIGVRKVMGAARSRLIRQFLAE
                     AIFMAMLAAVIALALTSVLLPAFNALTGKEITLPLKKPDLWIGMTGITLLTGLLSGSY
                     PALYLSGFSPIRVLKAALPSGTRGDRVFRQGLVVFQFVLSIILILSTILITRQIDFMK
                     NARLGYDRENLLYIPIEGDLGKKLDVFRTEANALPGIQNVTMLSSSPTVMTDGILSLN
                     WDGKDPAEHDRFIQESIGPGFLKTMKIALSAGRDFDLANFPTDSSGVIINETAASLMG
                     YKHPVGRVIYSGSYKAHIVGVVKDFHFQSLHDKIMPLVLEPGRIDRYSHILVRTQARK
                     TQTALAGLRQLCTQLNPAFPFTYQFSDLEYARLYRSEDVTGRLSIVFAALAIVISCLG
                     LLGLSLFTAEQRVREIGIRKVLGASVGSLFGLLSKNFMTLVGLSFLAGAPLGWWAMHQ
                     WLAGFAYQTNIPWWAFLITGAAVVTVALVTVCWQTLRAANANPVKSLRSE"
     gene            complement(6037..10143)
                     /locus_tag="VGQ51_08995"
     CDS             complement(6037..10143)
                     /locus_tag="VGQ51_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9036745.1"
                     /translation="MTSLTGMSVLLGQGPKLRFTHLSGEQGLSNSTIEAIFQDSRGFM
                     WIGTRDGLDRYDGHEMVIYRNDPGDTTTLSDSYARCIYEDREHVLWVGTVNGLNRLDR
                     GHDRWTRWKHRDGDSSSLSSSGVTAILEDAKDRLWIATYGGGLDLMDRKTGRIRAYQM
                     GGGPGSLRDDRILCMLEDNSGLLWIGTQTGLDLFNPATGVFKAMDNPAITNASGNTII
                     ALQQDRQGNLWVGTEDDGLYLFDPLRGSFMRWGHSDKDPSSLGNNMIKCMLTDRKGQL
                     WAGSINGGLNLFHAPSGSFFRYSYEPGNESSLSQRTVSALYEDRQGNLWVGTHRGGIN
                     IYSPGMYKFDLYRQEPSPNSLSYNDVKTFCEDRAGNIWVGTDGGGLDLFQRDQRVFKH
                     YRNDPFNDHSLGSNAVLDVMQDREGRIWVSTWGGGLNLFDPVHGVFTRNLNDPGNPHS
                     ISSNFVQKTFEDYSGRLWVATYYGGLNIFDPSTRQFTRVVDDPTGTTSLTGKNIVSLM
                     EDKVGRIWIGTDDGGLNCYHPDTRHFTHYFDTDQRMPDLRVLFCDSRGRVWVGQKGLY
                     LFDATRDSFRVATMHAGLAGEFIKGVAEDGQGNLWISTSNGLTQLNPDTYTFSKYNTG
                     DGLQGLEFEANACLKTKDGELFFGGINGFNCWYPGAIRANPFVPPVYITAIQLANRKV
                     IPGAEGSPLQEDITLTREIRLSYRQSSFSLNFSALNYTTPENNQYAYRLEGLDTAWNY
                     VGRENKASYTNLNPGEYTFRVKASNNDGVWNEEGASLRIIITPPFWKTDWFISVLIAL
                     GIAGLYLFYKFRTRLKLRELEARKREEIRQVQLQFFTNVSHEFRTPLSLILGPLEKMM
                     KDRDYPSHNRYFQAMHRNAHRLLSLINELMDFGKLESGSLKLGVQAGNLQIFMEELAE
                     EFRDWAIQKEIDFTLRQSGTTGEAWFDRQVLEKIVLNLLHNAFKYTKPGGKISLEALS
                     SLDGFNPSFANELVLKNEFRGSRYAWLRVADTGIGISASSIHHLFERYYRITESHLGS
                     GVGLALVKSLALLHKADIRVYSERLKGTEILIGIPVGETDYRKEEKRVVAAEGGVRLE
                     GLPYRAGPHSFDEPEVQEVHGVRADLPPVETILLVEDNDELRHFLKESLGTAYQVVEA
                     CDGQEGLAKAREMAPGMIISDVIMPGMNGLEFCKAIKQNIDTCHIPFLMLTARSTAAS
                     QVEGLDAGADYYFAKPLNISLLQLTIRNRFDQDRKLKERYRRDSQAEAMDLVHSERDR
                     EFMTRLLTTIDSHLANPEFDIEWLCHEMGMSRTRLYQKIKGISRQSIGDFIRTIRLKK
                     AVQIMTHEDVTLTEVMMRVGIQTQSYFTKAFKKEFGKTPGQFLQEMRK"
     gene            10299..>12137
                     /locus_tag="VGQ51_09000"
     CDS             10299..>12137
                     /locus_tag="VGQ51_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9036746.1"
                     /translation="MVKRLLARMHILLLLLFISGTLLAQRRTITGKVVDNKDGSPLSG
                     VTVQAKGDLKGGTTTGSDGSFTITVGENARILVFSFIGYAAQEHSIGAGPMTIGLSAG
                     EANLNEVVVIGYGTTRKKDLTGAVAVVDEKDFQKGTITTPEQMIAGKVPGVSIISNSG
                     QPGAGSTIRFRGGSSLNASNSPLIVLDGVPLNSDVAPGQKSAVPGAGSPLSFINPNDI
                     ESFTVLKDASAAAIYGARAAGGVIIITTKKGRGGELKINFRSNNSMFTLIKEVPVLNA
                     AQFKQVVNTYGTPDKVALLGTSNTNWQDQIYQTAFGTDNSVSISGGVKGLPYRLSVGY
                     QAQNGILKTDNLQRTSATLALTPVLFDKHLKVNLNVMGSMENARFGNQGAIGGAISFD
                     PTQPIYSKSPRFGGYWEWLDPTTASGLKALVGRNPLGLLEQHYDKSTPKRSIGNLQLD
                     YSLHFLPDLHANLNVGYDYSDGRGTTFVPDSAAESYLAGGNPSGQNNKYRSTNYNSLF
                     EFYFSYNKDLKNIRSHIDAQAGYSYNNYLSTVHNYAQYYADGSMVPKTTPPTASVDYQ
                     HTLISWFGRVTYSFEDKYLLTGTFRRDGSSRFAPPYRWANFPSAAFA"
BASE COUNT         2651 a         3303 c         3404 g         2779 t
ORIGIN      
        1 gacccggaaa gttccgtatc acgttgacgg acatacctgt cacagggtcc acgttgatag
       61 gcccctggtc cttggcggaa atatgctcct tcagatcgac agccgcggtg atgggccagt
      121 tggcgggggc ggtccagacg ccttgaagat tatccaccgt cgcatagacg ccgacgactc
      181 caggagcggc aggtaaaagc atctcgagat tgttggagat atagttcttt acggcataat
      241 agatctgtcc gcttttggcc ttcagatcac tcagcttggc ggggggagag ccggcggcgg
      301 cgcttttcag agataccttg ataaggtcgt ccctgtccag caacgtggtg gggtccttga
      361 cgctgggagt tatggtcgtg tatatcctcg gataataggc ggcggcatat ttgaagttct
      421 cgggcaaagg cccggtgacg gatatcccgg aattattcgt atcctgaccg cgcagtactt
      481 tgatgtcctc tatcggaagg gtatggccgt tgacgggata aacatccagc acggccatcc
      541 ggttccccat ttcgatacaa tggtcgatcg ccaggttcca cagggcgtaa taagtagacg
      601 cgcccttgtc ggggtctgca gtgtgggcga tgctcatcgc atccgggatg accaccagcg
      661 ttatatcgtt gtatttttcc agctgtggac agaaagtcgt cccgtccggt ttgaagtcgc
      721 tgacgaggat gacgggatcc tggggtttgt acaatccgac cgagaggata tagcaaggcg
      781 ccccgccgtt ggcaaaatat agttgcagcg aatagtacat caggtagggc gatcgtgtag
      841 gctcgtccag atcgacaatg accaccggct gggtcgggct gctatcatcg acggttgcat
      901 gcaatccggt ctccgggtag ggaagaccga aatattgctg gtattcgatg atattttcaa
      961 ccatgaaggg cacaccgaga agggaagtag tgccctccat tgccttttcc gtatacccga
     1021 cgaagacggg taccgccgtt ggtacggata cgatcgaggc tgggagaacg gggatctcct
     1081 ggatatatac gccgggagta tttagaggtg gaagggctgt ttgtgttgtt gtagacatat
     1141 catgaagttt taggtatttg attaattagt ttgcggtccg ccggtacgcg ctcccggtag
     1201 gggattgcgc aggcctgccc gcgcagcgcg gattaggtcg gcgcgggaga gacggggaag
     1261 atgcccgatt cgggtaccgg aatatcggag aaaagctgga agccgtcgac ttcgatcagc
     1321 gtatccggcg ccgggttggg tagatacgta aaaacgggag gcgtggcggc ggggttctgc
     1381 gaaaagaaaa agggaagata ggtgccgggg atgggaaaga gcgtggtcaa tacgcccgtg
     1441 gtccccgtta cgaaagtggc gatcgtgccc gtgggggctt tcaggatctt ggtagtgtct
     1501 tccgaaaaat aacgccagaa ggtatagcgg cttttgcaac ggatctcgaa gacggggaag
     1561 ggagacgtgg tctggtccgg agccgcgagt accggcatgt aaagattgcc gtttggatca
     1621 aggaggctca tgctgttgtt ggcgaccttc gcctgcagct ggatgacgcc ccatgtggac
     1681 gtaaggtcgg atgcgacgtc ataaaagatg agcggtatcg tcttcgtgta gccgccattg
     1741 ccggtgactt ccagcgaata cagtatcgcg tcggtggccc gggccttcgg caaggtgagc
     1801 agcggtgtta gcgccggatt gccggcgtcg tcgaagttga tcgtgacggt gcccagcggg
     1861 tcggtaccgg tgatcggcat cgtccgtacg ggattgccgc ccgggtcttt gagtacgaat
     1921 tgagcttgcg tgacattgtc cgactcctcg aaattgtagt cgaattggcg gccgacgatc
     1981 agccggtcgt tctcattgat gaatccgttg ccggggacgg tctcgaaggc cggcgtggta
     2041 ccgttataat agcaaagctg gacattggcc ccgctgagcg acagctcgcc ctgctcatac
     2101 gtatatccgg ggacgttagc agagatcggt ctcgacagga cgggcgccgt cttcgccgcc
     2161 cccagatccg cattgctgaa ataatatagc gatgatatcg gatggctcag ccggccattg
     2221 gtcagcgtgg cgaattgtcc ggtcccctgc tgcatgagga tgataagatt gaaattcccg
     2281 tccatcgata tggccggagt ataaaccgtc gtgcccgatt gaactgtctc attgacgcgg
     2341 acggccacct ggaacccgga aagactgctc agcaaccgaa ggtcctggtt tcggaattgt
     2401 gccgtcatgt aagaaggcaa tataaagctg aggtcgtcgc tgccggcggg ctggtcggcc
     2461 tggtaacgcc ggttcagcca cttcaggcgg tcggtctgct gcgcgttctg gaacaccgac
     2521 gtattgtcgg ggtccgtcag gtagtattcg tggaggacct tcacctcgaa caatatgtta
     2581 tacaatagcc tcatgatttg aatttcgtca gctgcgaaat atcggcatcg atctcgcgga
     2641 taagcggaac gttcctcagg atctttctgt cagtcagcgc tatgagcctc agcttgtaca
     2701 tcacgaaggg tatctgccga ccgccgagcg atccccatag atggttcagc tgctcgaagg
     2761 tcagggtata cagttccatg gtgaatctca ggccgaccag ctcggggtcg tcactgaatt
     2821 cattgcccga cgcgtcctgg taagaggccg atccgtagga gaaggtattc ttgccctgaa
     2881 ggaatttgac cacataggag atccgctgta gcgcatatat atatttgtca gcccagtaat
     2941 tgcaggtgaa aagaacatac agattcagat agacgggggg attctcatag gccgcggtgc
     3001 ccagctgcgg ataattcttg tacgcgggtt ggttcttcaa cgtgctctct tcttcgacgt
     3061 tgactaccgt aatgatgatg ctgttggaaa gcttgtctcc attggtcgtt tcgagaagac
     3121 ccacgttgtc cacgactacg gtggcagagg ggtctatcgg cgcgagataa ttttccagct
     3181 ccttttgcag caaagaaata gccgtatcaa tcataccgaa agattgaaca ataagggaat
     3241 gacaataaaa tgccgctgcg tcggtccgca cggacaggcg ggctaaggca gaagagggta
     3301 acggcagcta cgggtaatca ggtggatgat aaatgcagaa gtggcgcgga tgaacggtgc
     3361 ggtctagtgt tctaggataa acgaagtagt tggtagtagt tccggcaatt ttgtagttcc
     3421 ggttgcttta gcgaagttta ggaaattatt ttgaataaac aataccggaa cataagtaga
     3481 aatacgcaat tttttttgct atcgtcggat gacatactac taaccgggta ttactctact
     3541 cgcttcgaag actttttacc gggttggcgt tcgcagcccg caaggtttgc cagcatacgg
     3601 tgacaagcgc aaccgtcacc actgccgcgc ccgtgatcag gaacgcccac cacgggatat
     3661 tcgtttgata ggcgaagcct gcgagccatt ggtgcatggc ccaccagccc agcggcgccc
     3721 cggcaaggaa agacaaacca acgagcgtca tgaaattctt cgagagcagt ccgaataagg
     3781 agcccacgct cgctccaagc acttttcgga taccgatctc ccgcacgcgc tgttcggcgg
     3841 tgaacaacga tagcccgagc agacccagac aggagatcac gatggccagc gcggcaaaga
     3901 cgatcgacag ccgtcccgtg acatcctcgc ttcgatagag tctggcgtat tccaggtcgg
     3961 agaattgata ggtgaatggg aaggcagggt tcagctgggt gcataattgc cgtaagcccg
     4021 ccaaagcggt ttgcgttttc ctcgcctggg tccggacgag gatatgcgaa tacctgtcga
     4081 tgcgacccgg ttccagcacc agcggcatga tcttgtcatg aaggctctgg aaatggaaat
     4141 ctttcacgac gccgacgata tgagctttat agctgccgct atagatcacc cttccgacag
     4201 gatgtttgta gcccatcagg gatgccgccg tctcgttgat gatgacgccc gagctgtcgg
     4261 taggaaagtt cgccagatcg aagtcccggc cggccgagag cgctatcttc atcgtcttca
     4321 gaaaacccgg tccgatggat tcctggatga agcggtcatg ctctgccgga tctttgccgt
     4381 cccagttcag tgaaaggatg ccatcggtca ttaccgtcgg actgctcgat agcatggtca
     4441 cgttttgtat gccgggaagc gcattcgctt cggtacggaa gacatcgagc ttcttgccga
     4501 gatcgccctc gatcgggata tagagcagat tctcgcgatc gtaacccaga cgagcgttct
     4561 tcatgaagtc gatctggcgt gtgatgagga tcgtggagag aatgagaatt atggacaata
     4621 cgaattgaaa tacgaccaat ccctggcgaa acaccctgtc accccttgtc cccgatggca
     4681 gcgcggcctt gagcacgcgg atgggactga acccggacag atataaggca ggatagctgc
     4741 cgctgagcag ccccgttagt agcgtgatac cggtcatgcc aatccataga tccggctttt
     4801 ttaaaggcag cgtgatctct tttccggtga gggcattgaa ggctggcagc agtacgctgg
     4861 tcagtgccag tgcgatcacc gcggccagca tggccatgaa gattgcttcc gcaaggaatt
     4921 ggcggatcag ccggctcctg gctgcgccca tgaccttgcg cacgccgatc tctttggcgc
     4981 gtcttaccga acgcgccgtc gtcaggttca taaaattgat gcacgcgata accaggatga
     5041 acagggcgac gaggctgaat agccggacat aagcgattcg gccttctttc ggcacgccgt
     5101 cgcggaattc gttatgcagg taatagtcgc tgaaacgttg cagcccaagt tcgatgcgga
     5161 tatccttcac ctccgtattg aacgggtccg tcagatgact gatcttcttt tccaccttcg
     5221 cctcgtcggc cccaggtttc agcaggatca cggtacgcgg gtcgaccgcg ccccaacgtt
     5281 tggcccatcc gttgtcttcc acatagcccg accagctcat aatataattc gccggactgc
     5341 tcacggcgga ggtgagatcc tcgaagactg ctttgaccgt gaagttcctc ctgttctcat
     5401 attttatggc cttgccgata gccgccgcgg ggctgccgaa gaaggcgatc gccatcttgc
     5461 gcgagatggc caatgcgtcc ggtgagctca gcgcgtccat tgcattacct tccaccaacg
     5521 gatagctgaa catgctaaag tagtcggcac ctgccgcgaa gccgtcctct ttcaggatct
     5581 tgccccccac ggaaaaattg ttggtgttcg accacgacat cggtgtcgcc gcctccactt
     5641 ccggaatttc ttttatcaat tcctccgcca gcggcccttg tgtccaatac caggtctgta
     5701 gctttccacc catatagttc cgctcgtaga catagtacag acggcgaccc ttggcatgaa
     5761 aggcgtcgac tcctctttcg tcctgcaccc ataggaggat cagcagactg caggctattc
     5821 cgagtgccag gccggcgaga ttgataaaag tgaagccggg ctgtttgcgg agattgcgta
     5881 tggcggtttt gatgtagttc tttagcatga aagatggaca gccaaaatga taccgggttt
     5941 gctagctatt ctgaatcaaa cgcttgcaag ccatagtcga cccgggactg tacggtttcg
     6001 ctacagcggt tgtgtcgtct caggcagcgg gtccgcctac ttcctcatct cctgcaaaaa
     6061 ctgccccggc gtcttcccaa actccttttt aaacgccttc gtaaaataag actgcgtctg
     6121 tatccctacg cgcatcatca cctccgtgag cgtaacatcc tcatgcgtca tgatctgcac
     6181 cgccttctta agccggatcg tcctgataaa atcaccgatc gattgtctgc tgatgccttt
     6241 aatcttctga tagagccgcg tccggctcat acccatctca tggcagagcc attcgatgtc
     6301 gaactcggga ttggctaagt gcgagtcgat ggtggtcagc agacgggtca tgaattcgcg
     6361 atcgcgctcg gaatggacga ggtccatggc ttctgcctga gagtcgcggc ggtaacgctc
     6421 cttgagcttt ctgtcctggt cgaagcggtt gcggatcgtt agctgaagca gcgagatatt
     6481 caacggcttg gcgaaatagt agtctgcgcc ggcatcgaga ccttctacct gagaggcggc
     6541 cgtgctccgc gcagttagca tgaggaatgg gatatgacag gtgtcgatgt tctgtttgat
     6601 ggccttgcag aattcgagac cgttcatgcc gggcatgatc acgtcgctga tgatcattcc
     6661 gggcgccatt tccctggcct tggccaatcc ttcctgccca tcgcaggcct cgacgacctg
     6721 gtaggccgta ccgagcgact ccttcaggaa atgccggagc tcgtcattgt cttctaccag
     6781 caggatggtt tccacgggcg ggagatcggc gcgcacgccg tggacctcct gtacttcggg
     6841 ctcatcgaag gaatgcgggc ccgcacgata ggggagacct tcgagacgta cgcccccttc
     6901 ggctgcaacc acccgttttt cttccttgcg gtagtcggtc tctccgaccg ggatacctat
     6961 gaggatctcc gttcctttca gccgttcgct atacacccgg atgtcggcct tgtgcaacaa
     7021 cgcgagactc ttcaccagcg cgagccctac acccgaaccg agatgcgatt cagtgatccg
     7081 gtaataccgc tcgaacagat ggtggatcga agatgcagaa ataccgatac cggtgtcggc
     7141 cacccgtaac caggcatagc ggctcccccg gaattcgttc tttagcacca gctcattggc
     7201 aaatgacggg ttgaacccgt ccagcgagga caaggcctcg agcgatatct tccctcccgg
     7261 cttcgtatac ttgaaggcgt tatgcagcag gttgagcacg atcttttcca gtacctggcg
     7321 gtcgaaccag gcttcgccgg tggtcccgga ctgacgcagc gtgaaatcga tctctttttg
     7381 tatggcccag tcgcggaatt cttccgccag ctcctccatg aagatctgga gattgccggc
     7441 ctgtacgccc agtttcagcg acccgctttc cagcttgccg aaatccatca gctcgttgat
     7501 caggctcagc agccggtgcg cattccggtg catcgcctgg aaataccggt tatgcgaggg
     7561 atagtcccga tctttcatca ttttttccaa cggtcccaat atcagcgaca gcggcgtccg
     7621 gaattcatgc gagacattgg tgaagaactg tagctgcacc tggcgtatct cctcgcgttt
     7681 gcgcgcctcc agctcgcgca gcttgagccg cgtccggaat ttgtagaaaa gatataaccc
     7741 ggcgatgccg agggcgatca gcaccgagat gaaccagtcc gtcttccaga atggcggtgt
     7801 gatgatgatc cgcaaagaag cgccttcttc gttccatacc ccgtcattat tggaggcctt
     7861 tacccggaaa gtatattcgc ccgggttgag gttcgtatac gatgccttgt tctccctgcc
     7921 gacgtaattc catgccgtgt cgagcccttc cagacgatag gcatactggt tgttctcggg
     7981 cgtggtatag ttcaacgcgg aaaagttgag cgaaaagctg gactgccggt aggagagccg
     8041 gatctcgcgg gtcagggtga tgtcttcctg caacggcgat ccctcagcgc cggggatcac
     8101 cttccggttt gccagctgaa tagcggtgat gtacacaggt ggcacgaaag ggttggcccg
     8161 gatagccccc gggtaccaac agttgaaccc atttattccc ccgaagaata actccccatc
     8221 ctttgtcttc agacaggcat tggcctcgaa ctccaaaccc tgcaatccgt ctccggtgtt
     8281 gtatttcgaa aatgtatagg tatcggggtt cagttgggta agcccgttgg aggtggatat
     8341 ccatagattg ccctgaccgt cttcggccac cccttttatg aattccccag ccagtcccgc
     8401 atgcatcgtg gccacccgga aactgtcacg cgtggcatcg aaaaggtata atcctttctg
     8461 acctacccaa acccggccgc ggctatcgca aaacaatacc cgcagatcgg gcatccgctg
     8521 gtcggtatcg aaataatgcg tgaaatgccg ggtatcggga tgatagcaat tcaaacctcc
     8581 gtcatccgtg ccgatccata tccgtcctac tttgtcttcc atcagtgaga cgatattctt
     8641 tcccgtcagg ctggttgtac ccgtagggtc gtcgaccact cgggtgaact gtcgggtgct
     8701 gggatcgaag atattcagtc cgccgtagta ggttgctacc cacagcctgc ccgaataatc
     8761 ctcgaaggtc ttctgcacga aattgctgct gatggaatgc gggttgccgg ggtcgttgag
     8821 atttcgggtg aagacaccgt gtacaggatc gaagagattc agacctcctc cccatgtcga
     8881 gacccagatt cgtccttcac ggtcctgcat aacgtccaac accgcattcg agccgagact
     8941 atggtcatta aagggatcgt tgcggtaatg tttaaagact cgctgatccc gctggaacag
     9001 gtcgagaccg ccgccatcgg tgccgaccca tatattcccg gccctgtcct cgcaaaaggt
     9061 tttgacatcg ttgtagctga gactgttcgg ggaaggctcc tggcgataga gatcgaattt
     9121 gtacatgccg ggagaataga tattgatccc gccgcggtgt gtacctaccc atagattgcc
     9181 ctgccggtct tcatacaagg cggacaccgt ccgttgtgag aggctgcttt cgttgcccgg
     9241 ctcataagaa taacggaaga aggacccgga aggcgcatgg aaaagattca gcccgccatt
     9301 gatgctgccg gcccaaagct gtcctttgcg gtcggtgagc atgcatttga tcatattgtt
     9361 acccaggctg gaagggtcct tgtccgaatg cccccatctc atgaagcttc cgcgtaatgg
     9421 atcgaaaaga tatagcccgt catcctcggt gcctacccat agattgcctt gccggtcctg
     9481 ctgcagggct atgatggtat tcccgctggc gttggtgatc gccgggttat ccatggcctt
     9541 gaagaccccc gtcgcggggt tgaacaggtc cagccccgtt tgtgttccta tccaaagaag
     9601 accgctgttg tcttcaagca tgcaaaggat ccggtcatcg cgcagactgc cgggtccacc
     9661 tcccatttgg taggccctga tccgcccggt ctttctgtcc atcagatcga gcccgccgcc
     9721 ataggtggcg atccataacc ggtctttggc atcctccagg atcgccgtga caccgctgct
     9781 gctgaggctt gaactgtcac cgtcgcggtg tttccatcgt gtccagcggt cgtgtccgcg
     9841 gtccagtcgg ttgagaccgt tgaccgtccc gacccagagc acatgctccc tgtcttcata
     9901 gatgcagcgg gcgtagctgt cgcttaaagt ggtggtgtcg cccggatcat tgcggtagat
     9961 gaccatctca tgcccgtcat aacgatcgag accgtcgcgc gtaccgatcc acatgaatcc
    10021 gcggctgtcc tggaatatgg cttcgatggt gctgttggat aatccctgct ctcccgacag
    10081 gtgggtgaat cgaagtttgg ggccctgccc cagcaggaca ctcatcccgg taagagatgt
    10141 cataatatat atggagacag ctttccacat ggcgaacagg tccgggtcaa atgtaatgtc
    10201 attcggcgga atagtcaatt tgaacaatag aaatgccgga tagaacaacg gcctcaatcc
    10261 gccccctgct accttgtggg tccaataatt aactagttat ggtaaaacga ttgcttgcaa
    10321 ggatgcatat ccttctatta ctgctattta taagcggcac cctcctcgct cagcgaagga
    10381 ccattaccgg gaaggtggtg gacaacaagg acggctcgcc cttgtccggc gtcaccgtcc
    10441 aggcgaaggg cgatctgaaa ggcggtacga ccaccggtag cgacggcagc ttcacgatca
    10501 ccgtcggcga gaatgcccga atccttgttt ttagtttcat cggttatgcc gcgcaggagc
    10561 attccatcgg cgcagggccc atgactatcg gtctttctgc gggagaagcc aatctcaacg
    10621 aggtcgtcgt catcggttat ggtaccaccc ggaaaaaaga tctcaccggg gctgtcgccg
    10681 tcgtggatga aaaagacttc cagaaaggaa ccatcaccac tcccgagcag atgatcgccg
    10741 gaaaagtacc tggtgtctcc attatctcca acagtggtca gcccggagcg ggtagcacga
    10801 tccggtttcg cggcggatcc tcgctgaatg ccagcaacag cccgttgatc gtgctcgatg
    10861 gggtaccgct caacagtgac gtggcccccg gacagaaaag cgccgtaccg ggtgccggca
    10921 gtccgctcag tttcatcaat cccaacgaca tcgaatcctt caccgtgcta aaggatgcct
    10981 ccgcggcagc catctatggt gctcgtgcgg caggcggggt gatcatcatt actaccaaaa
    11041 agggcagggg aggcgaactg aagatcaact tccggtcgaa taattcgatg ttcaccctca
    11101 tcaaggaagt gccggtcctc aatgctgccc agttcaaaca ggtggtcaac acctatggca
    11161 ctccggacaa agttgccctg ctgggtacgt ccaacaccaa ctggcaggac cagatctacc
    11221 agacggcttt tggaacggac aattccgtca gtatcagcgg aggggtgaag ggtctgcctt
    11281 accggttgtc ggtcggttac caggcgcaga acggaatcct taaaacagat aatctccagc
    11341 ggacctccgc gacccttgca ctgacgccgg ttctttttga caaacatctc aaggtcaatc
    11401 tcaacgtgat gggctctatg gagaatgcgc gtttcggcaa ccagggggcc atcgggggag
    11461 cgatcagctt cgaccccaca cagcccatct attccaagag tccccggttc ggcggttatt
    11521 gggaatggct ggatcccact acggccagcg gtctcaaggc actggtagga cgcaacccac
    11581 tgggtttgct cgaacaacat tacgacaagt ctactcccaa acgcagcatc ggcaacctgc
    11641 agctggatta ctcccttcat tttctgcccg atcttcacgc caacctcaac gtgggttatg
    11701 actattccga cggccgcgga acgacatttg tccccgacag cgcggcggaa tcctatctcg
    11761 caggcggaaa ccccagcggt cagaacaaca aatacaggag caccaattac aatagtcttt
    11821 tcgaatttta tttcagctac aacaaggacc tgaaaaatat caggagccat atcgatgccc
    11881 aggcgggata ttcatacaat aattatctta gcaccgtcca taattatgcg cagtactatg
    11941 ccgacgggtc gatggtcccc aagacgacgc cgcctacggc cagcgtggac tatcagcata
    12001 cgctgatctc ctggttcgga agggttacct atagtttcga agacaaatac ctgctcacgg
    12061 gtaccttccg ccgtgacgga tcgtcacgtt tcgcgcctcc ctaccgctgg gctaatttcc
    12121 cgtcggcggc cttcgcc
//
LOCUS       DASYSV010000301         4394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_58987, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000301 DASYSV010000000
VERSION     DASYSV010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58987"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VGQ51_09005"
     CDS             <1..660
                     /locus_tag="VGQ51_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEV9036747.1"
                     /translation="AIVRQLQARYPGRLFLEERQGKLGLGTAYIHGFKWAVRNNYQFI
                     FEMDADFSHNPRDLDRLYEACKRQGGDVAVGSRYVKGGGNVNWPWDRVLLSRGGSLYT
                     QLITWMPVQDPTAGFVCYKKEVLETINLDQIRFVGYAFQIEMKFAAWKLGFKIKEVPI
                     VFEDRKFGVSKMNKGIVKEGILGVLNLRWQSLFKNYRNRVKNDVMAMTSFSKEAIAES
                     K"
     gene            664..1545
                     /locus_tag="VGQ51_09010"
     CDS             664..1545
                     /locus_tag="VGQ51_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV9036748.1"
                     /translation="MKKAFIQLHIAVLLAGLTGILGKLISLNAGLLVWYRLLLTAPSL
                     WLLALVRRQSVRIDRRDLWRIFGIGSIAAMHWVAFYASIKYSNVSVGLLCFSAVGFFT
                     AFLEPLILRHRMDIAELFLGLLVIAGIFCIFQVDPHYKTGILIGLVSSLLGSLFPVLN
                     KRILRRVPSETVTLYELSGGFLALTILMPLYLYLFPAPTLLPDLQDWVWLLILAWACT
                     VLAFNLSMAALNKISAFTVNLSYNLEPVYGILLAFVLFREDKYMNSGFYVGMALILLS
                     IILQTMRLWRRQLRPTA"
     gene            complement(1549..2922)
                     /locus_tag="VGQ51_09015"
     CDS             complement(1549..2922)
                     /locus_tag="VGQ51_09015"
                     /inference="COORDINATES: protein motif:HMM:NF020768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031221 - arabinan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosidase catalytic domain-containing
                     protein"
                     /protein_id="HEV9036749.1"
                     /translation="MIRTIALPQITIFVIFLALNTQAQNILDKTGLGAGATASAAYSS
                     RLLSSSYAGSALQVRRSSDNSTQDIGFTANGDLDTAMLKTFVGAGDAFISAWYDQSGN
                     GKDLTQAAQANQPQLLSGGTIYRENDQPFIRFFGTGGADYRSLNLAADMTTVGHVSAV
                     LRFSSTGDGFILSHTADYYWHSEPYTALINSTYASASVQGGNGWENGNAYAPTSMPWP
                     STLTIAELEPSTPSTNVHWNNIGSDRNQYHHISQGGGYGELILFSTALSTADRQTLEA
                     NEQNYFTALPLPVTWLSFTAQASTDDVHLQWQTASEQNSKNFQVQYSADGSSWTILAT
                     VPAAGNSSSVCSYHYIRYDAPAGENYYRIAETDLDGKTTYSDIRTVRIDPAEAEFKVI
                     QNPVIGGLLHVNVNRPTILSLFSVDGDLLWRKHYDRGTAEIPMNGDGPGEYILAGKTL
                     SVKVLVK"
     gene            3085..>4394
                     /locus_tag="VGQ51_09020"
     CDS             3085..>4394
                     /locus_tag="VGQ51_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036750.1"
                     /translation="MQCIFLAFIIGNRCGLAQQVRCADTVRTVLHTAPLFQPNLPFSS
                     LATKSGGAAIFQSVTSPGTKSLLLYKSDASGNPDWAKKITAASSVPWDNLKQLDDGTI
                     VINSQAYRVLSQSLGSSHLLVDTGGDVIWQGDYTNQDQDSGIQVMNSGTTGTIIWAGT
                     RLLSNGGGITIQYFDYVNEAELWRRNFGLNNSSFIQLFLCGVVYKNNQVYVAGVYLDT
                     QSGALNKGIWLLGLDASTGTILEAISYQIADPPDGSNSLLPTGTGRLMNGSANGLVLS
                     VELPDQVTNAKGIAFFSMDGYLRIKHPTLCLSGNSGESGSSSALMNIAGDALYSFIPA
                     TGGGYFAAIDSGNNVSRQYKVTYSQGQGGNAAYDLADNGSLVMVAPVVQGAQLSFEVT
                     GIPPDLTTNDACINITDTPFVQQDPVNILPSSLQWIAQSSLPKTT"
BASE COUNT          948 a         1030 c         1258 g         1158 t
ORIGIN      
        1 gccattgtca gacagctgca ggcgagatat ccgggccgcc ttttcctgga agaacgccag
       61 ggcaagttgg ggctgggtac cgcctatatc catgggttta aatgggccgt ccggaacaac
      121 tatcagttca tctttgaaat ggatgctgat ttttcgcata accccaggga tctcgaccgg
      181 ctttatgagg cctgtaagcg tcagggcggc gacgtagccg tgggctcgcg gtatgtgaag
      241 gggggcggta atgtcaactg gccctgggac cgcgtcctgc tctctcgcgg aggttccctg
      301 tatacgcaac tcatcacgtg gatgcctgtc caggacccca ctgcaggttt tgtatgctat
      361 aaaaaagagg tgctggagac cataaatctc gatcagataa gattcgtcgg ttatgccttc
      421 cagatcgaaa tgaaatttgc cgcgtggaaa ttggggttca agataaagga ggttccgatc
      481 gtgtttgaag accggaaatt cggtgtttca aagatgaata agggaattgt gaaggaaggg
      541 atcctggggg tgctcaattt acgctggcag tccctcttta aaaattaccg caatcgggta
      601 aagaacgatg taatggctat gacctctttc tccaaggaag ccatcgctga aagcaagtaa
      661 gccgtgaaga aggcattcat tcagctccat attgccgtcc ttcttgccgg gcttaccggg
      721 atattgggta agcttatctc tctcaatgca ggtttactgg tgtggtatcg acttttgctg
      781 acggcgcctt cgctgtggtt attggcattg gtgcgtcgtc agtcggtgcg tatcgacagg
      841 cgtgatctct ggcgcatttt cgggatcggg agcatcgccg ccatgcactg ggtcgccttc
      901 tatgccagta tcaaatattc caatgtatct gtaggattgc tgtgtttttc tgcggtggga
      961 ttttttaccg cttttttgga gccgctgata ttgagacacc ggatggatat cgcggagctg
     1021 ttcctggggt tgctggtcat cgccggtatc ttttgcatct tccaggtcga cccgcattat
     1081 aagacgggta tcctcatcgg tctggtctcg tcgctgctgg gaagtctttt cccggtgctg
     1141 aataaacgca ttttgagacg cgtgccatcg gagacggtta cgctctatga actaagcggg
     1201 ggatttttgg cgctgacaat attgatgccg ttgtatctat atctatttcc cgcccccacg
     1261 ctgttgccgg atctgcagga ttgggtatgg ctgctgatcc tcgcatgggc gtgtacggtg
     1321 ctggctttta atctttcgat ggcggcgctc aataagatat ccgcgtttac ggtgaatctg
     1381 agttataacc tcgagccggt gtatgggata ctgctggctt ttgtgctgtt ccgggaggat
     1441 aagtacatga acagcgggtt ttatgtgggg atggcgctga tcctgctgtc gatcatcttg
     1501 cagacgatgc ggttgtggcg cagacaattg cggccgacgg cctgacacct atttcaccaa
     1561 cacttttacg ctcagcgtct ttccggcaag gatatattct ccggggccat cgccgttcat
     1621 cggtatctct gcggtacccc gatcgtaatg ttttctccaa agcaaatccc catctactga
     1681 aaacagggac aaaatcgtcg gcctgttcac gttgacatgc aggaggccgc cgatcacggg
     1741 gttttgaatt actttgaatt cagcctctgc ggggtcgatc cggaccgtcc ttatatcgct
     1801 gtacgtggtt tttccgtcga ggtccgtttc ggctatccgg taataatttt cgccggccgg
     1861 cgcgtcgtaa cggatatagt gatagctgca gacgctgctg ctgtttccgg cggctggaac
     1921 cgtggccagg attgtccagg aagatccgtc agcgctgtat tgtacctgga aattttttga
     1981 gttttgttcg gaggccgttt gccattggag gtggacgtcg tctgttgaag cctgcgcggt
     2041 gaaagagagc caggtgacgg gcagcgggag ggcggtgaaa tagttctgct cgttagcttc
     2101 gagcgtttgg cggtcggcgg tggagagtgc ggtggaaaac aggatgagtt cgccatatcc
     2161 gcctccctgg ctgatgtggt gatattggtt gcgatcgctg ccgatgttgt tccaatggac
     2221 gttggtagag ggggtggacg gttccaactc ggcaatggtg agggtcgaag gccatggcat
     2281 gctggtcggc gcgtaagcgt ttccgttctc ccaaccattg ccgccctgca ccgaagcgga
     2341 agcataggtg ctgtttataa gcgcggtata gggttcggaa tgccagtaat agtccgcggt
     2401 atgcgaaagg atgaatccgt cgccggtgga gctgaaccgt aacacggcag acacgtgccc
     2461 tacggtggtc atgtccgcgg ccaggttgag cgaccgatag tccgctccgc cggtgccgaa
     2521 gaagcggatg aacggctggt cgttctcccg gtagatggtc ccgccgctta ataattgagg
     2581 ttggttggcc tgggcagcct gggtgaggtc cttaccgttg ccgctttggt cgtaccaggc
     2641 ggagatgaag gcgtcgccgg ccccaacgaa ggtctttaac atggcggtgt cgaggtcgcc
     2701 gttggcggtg aagccgatgt cctgtgtgct attgtcgctg ctacggcgga cctgcaaggc
     2761 gcttcccgcg tacgaactgc taaggaggcg gctgctgtat gcagccgagg ctgttgctcc
     2821 ggcgcccaga ccggtcttgt cgaggatgtt ttgggcttgc gtgttgaggg cgaggaaaat
     2881 gacgaagatg gttatctggg gtagagcgat tgtccggatc acattaaagg attgtggcat
     2941 taaaactacg acaatatcgt acaatcgcca catattaaat aagacgtatg actgcctttt
     3001 taagcaataa atgcctatct gtccgttata tagccgatgc cgagtagacc ttattttccc
     3061 ttttggcgaa gactatggaa gacaatgcaa tgtattttcc tggcatttat tatcggcaac
     3121 aggtgcgggc tcgcgcagca agtgaggtgt gcggataccg ttcggacagt tctgcatacc
     3181 gcccctttat ttcaacctaa tcttcccttt tcttctttag cgacaaaatc cggaggggct
     3241 gccattttcc aatccgtgac cagcccgggg acgaaatcgt tattgcttta taagtccgat
     3301 gcctcaggga atccggattg ggccaaaaag ataaccgcag caagctcagt tccatgggat
     3361 aatttaaaac aactggatga cgggactatc gtaataaata gccaggcata cagggtgctt
     3421 tcgcaatccc taggcagtag tcatctcctc gtggacaccg gtggggatgt tatctggcaa
     3481 ggcgactaca ccaaccagga ccaggactcg ggcatacagg ttatgaattc cggaactacc
     3541 ggaacaatta tctgggcagg aacacgtctt ttgtccaatg gcggaggcat taccattcaa
     3601 tatttcgatt atgtcaatga ggctgaactc tggcgcagga attttgggtt aaacaattcc
     3661 tcctttattc aattgtttct ttgcggtgtc gtatacaaaa acaatcaagt ctatgttgcc
     3721 ggcgtctacc tcgatactca atcgggtgct ttaaataagg ggatttggct gcttggattg
     3781 gatgccagca cgggaactat cctggaggcc atatcgtatc agattgcgga tccgcccgat
     3841 ggatcgaaca gcctattgcc cacgggtacg ggtcgattga tgaacgggag cgctaatggc
     3901 ctggttttat ccgttgaatt accggatcag gtgacaaatg ctaaaggcat tgcctttttt
     3961 tccatggatg gctatcttag gatcaaacac cccacgttgt gtctatccgg caattcaggt
     4021 gaaagcgggt cctcttccgc cctaatgaat atcgccggtg acgcattgta ttcctttata
     4081 ccggcaacag gcggaggtta ttttgcagca atagattccg ggaataatgt ttccaggcaa
     4141 tataaagtaa cctatagcca gggacaggga ggaaatgcgg cttatgattt ggctgataac
     4201 ggcagtctgg tgatggtggc tccggtcgtg caaggagcac agctttcctt tgaagttaca
     4261 ggaataccgc ctgatctgac aacgaacgat gcttgtatca atattacaga tacccctttc
     4321 gttcagcagg atccggtgaa tattttgccg tcctcgcttc agtggatcgc acaaagcagc
     4381 ctgcccaaaa cgac
//
LOCUS       DASYSV010000302         3007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_183090, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000302 DASYSV010000000
VERSION     DASYSV010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183090"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..813)
                     /locus_tag="VGQ51_09025"
     CDS             complement(217..813)
                     /locus_tag="VGQ51_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV9036751.1"
                     /translation="MVSAILLAAGSSQRMGAVNKLLLPWRDNTIVATVAGHLLAAGLE
                     EIIVVTGHQAPGIESALQSMPVRFVHNPSFATGLTGSIQKGVSIAKADGYMICLADMV
                     LVTPEEYSLLSAAFQERRALDDRCIILPEFKDTIGNPVIFSAAYRSDILQHPEKEGCK
                     SLVRSNKDHHLRVHMPSDHVLRDIDYPDDYKALTPTPP"
     gene            869..1582
                     /locus_tag="VGQ51_09030"
     CDS             869..1582
                     /locus_tag="VGQ51_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEV9036752.1"
                     /translation="MNRLLTTWLSRIVLVSCLLCGGWRLSAQNPAVAASSSVVSTQPP
                     FWNEIAEFKHKDSLQHPPANAILFIGSSSFRKWTGVQTDFPGYTIINRGFGGSTFDDL
                     IRYAKDIIYPYHPRQVVIYCGDNDLAEGEKGKKVYKRFVKLYDMIRKRLGNVDIVYVS
                     IKPSPSREKLMSEMEDVNDMIRNFMAERSHAAFIDVYHLMLNAHGHPMDELFIADKLH
                     MNEKGYKIWQKAILPYLDK"
     gene            2158..2622
                     /locus_tag="VGQ51_09035"
     CDS             2158..2622
                     /locus_tag="VGQ51_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036753.1"
                     /translation="MAYANTQEQETSILKAKISELKSALFFTESKSIVKLPTHVISEV
                     EMDAEGQIWFVIPKPAMHIEAYDREIPAKLDFFKKGKDFFVKIRGIGYLQTDSVEALN
                     AGLSAEMQHRMSDEAVILVKVKIEETDLVDNTPKPAQNWLQMSRTQLSSWFF"
BASE COUNT          747 a          656 c          918 g          686 t
ORIGIN      
        1 tttttcgact ttataaacta tgaaaaactg gagacattcc gtgattttgg cgggatcagg
       61 atagaagagg attttgtgat cacggaagac gggagcaagc tgttggggaa gccgttggcg
      121 aaaacggcgg aagcgataga agcgctcagg gggtgaggct ggtggctatc attgggggtg
      181 aagccggtgg tgatcatggg gtgaagccgg tggcgatcat gggggcgtgg gagtaagcgc
      241 cttatagtca tcggggtagt cgatgtcccg caggacatgg tccgagggca tgtgtacgcg
      301 aaggtggtga tccttgttgg agcggacgag ggatttgcag ccttcttttt cgggatgttg
      361 cagaatatcc gagcggtagg ccgccgaaaa gatcacaggg ttgccgatag tgtctttgaa
      421 ttcgggaagg atgatgcaac ggtcgtcgag agcgcgtcgc tcctggaatg cggcggacag
      481 gaggctgtat tcttcagggg tgacgaggac catatcagcg agacagatca tataaccgtc
      541 ggctttagca atgctgacgc ctttctgtat agagcccgta agaccggttg caaaggatgg
      601 attgtggaca aaacggacgg gcatggattg caacgccgac tctattccgg gggcctgatg
      661 gcccgtgacg acgatgatct cttcgaggcc tgccgcgagg agatggccgg cgaccgtggc
      721 gacaatagta ttgtcgcgcc aggggagcag caatttatta acagcgccca tacgctgtga
      781 agagccggcg gccagaagga tagcgcttac catgcgtgaa agggaagtta caacaataat
      841 gcttagcttc gtggcatagg gaaatcatat gaatcgactc ctgacgacct ggctttccag
      901 gattgtattg gtctcttgtc tgctttgtgg cggatggcgg ctgagtgcgc aaaatccggc
      961 ggtcgctgcc tcttcgtccg tcgtatcgac acaaccgcct ttctggaatg agatcgccga
     1021 attcaagcat aaggacagtc ttcaacatcc tcccgccaat gccatcctgt tcatcggcag
     1081 cagctcgttc cgcaaatgga ccggcgtaca aacagacttt cccggctata cgatcatcaa
     1141 ccgtggtttc ggcgggtcta ccttcgatga cctgatccgg tatgcgaagg acatcatcta
     1201 tccctatcac ccgcggcagg tggtgatcta ttgcggcgac aacgatcttg cggagggtga
     1261 gaaaggaaag aaagtgtata aacggtttgt caagctgtat gacatgatcc ggaagcgtct
     1321 tggcaatgtc gatatcgttt acgtatctat aaagccgagt cccagcaggg aaaagctgat
     1381 gtccgaaatg gaggacgtca atgatatgat ccgcaacttc atggcggagc gcagccatgc
     1441 ggcattcatc gacgtttatc acctgatgct gaacgcacat ggacatccga tggacgagct
     1501 gttcatcgcg gacaaattgc acatgaatga gaaggggtat aagatctggc aaaaggcaat
     1561 acttccctac ctggacaaat gagtctaatg tctcccggcg aagaagccgg cacagcctgt
     1621 atttcagcgg gtgatgggat gggcgcgccg cctgataaac cggccaggaa ggaaggagat
     1681 gggcgtgcgc gggaaggaat ctgcaataca gcaataaata tatatggcct ggttctatct
     1741 tgttctggcg tccttgttcg agatggcgtg gacgttccat tcattggcag gctttattca
     1801 gtcactcttc aaattgggga acattggcgc ctctgctcgg atacgtggtg ttcgggctgg
     1861 gcagtgtcta ttgtctctcg tagggcatga aagctatccc ggcgtccacc gcgttggccg
     1921 tttggatggg aacggcattg gtgggtgtga agctgtgcgt cgaattgagg aatggtcaaa
     1981 ttttggaaaa cgaatcccgt tttgggacga tccggctccc caaggccgcc tctaatgcgc
     2041 tgataataaa tattttacgg attggtatgc caattgtctt cttctaaccg tccgcagcca
     2101 acggaacaaa ggcagcggag gacggaaaca aaataatcta tacccaacat taaaactatg
     2161 gcatacgcta acacccagga acaagagacc agcatactga aggccaagat ctcggaattg
     2221 aagagtgcat tgttctttac tgaaagcaaa tcgatcgtaa agctcccgac gcacgtgatc
     2281 tccgaggttg aaatggatgc cgaaggtcag atctggttcg tgatccctaa gcctgccatg
     2341 catatcgaag cgtatgaccg tgaaattccc gccaagctgg atttctttaa gaagggaaag
     2401 gatttctttg taaagatcag gggtattggg tatttgcaga ccgatagtgt agaagcgctc
     2461 aatgcaggtt tgtctgcaga gatgcagcat cggatgagcg atgaggcggt catcctggta
     2521 aaggtgaaga tcgaggaaac cgatctggtg gataacactc ccaagccggc tcagaactgg
     2581 ctccagatga gcaggacgca attgtccagc tggttcttct aggccgtagg tgttgggtga
     2641 ttaaaaaagc atactgcgcg tgttacatat aggaagctga gtccgcgact tgttcgcgga
     2701 tttcagtttt caggaggtaa ccgagcccca ggcgaaggtt acgaacgagg aggagcgtgg
     2761 cgaagcagct ccgacgaagt tctataactg ttaggtatat gcgaaaaatg tgatgatcgg
     2821 cgggggcgta ggtgggggcg atgctggcgg aggggcgatg ctcgcggagg ggcgatgcgt
     2881 aggcgggggg cggtgccggc gggcggtgcg gaaaaaaaaa ggtccactgt agaaacagtc
     2941 gacctctaac gattgcttgc catatgaaaa aaggtagtat tctaatttat tggaacactg
     3001 cagttta
//
LOCUS       DASYSV010000303         7013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_698336, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000303 DASYSV010000000
VERSION     DASYSV010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698336"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VGQ51_09040"
     CDS             <1..691
                     /locus_tag="VGQ51_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV9036754.1"
                     /translation="SPDNYYNYMGDGAVDFDEKISLVAAQIREARVKMGFKNPIYLSV
                     DEYGAFARNFMSVLPVAQCLNSFIRHADVVRMANFTMLTSLLSSDPRNGTFRSPVFYT
                     FQLFSNNCLGASVDAYVACDTFGTEKHKGIPYLDVTAVYSKVNGVLYINVVNRHQSRP
                     IAADISAASGNFSGKAEASIINSDDLKAAFTYEHRSQYMPVAAPVNVNGNKLQYTFPP
                     HSFTQIKVTIK"
     gene            complement(697..3288)
                     /gene="xyl3A"
                     /locus_tag="VGQ51_09045"
     CDS             complement(697..3288)
                     /gene="xyl3A"
                     /locus_tag="VGQ51_09045"
                     /EC_number="3.2.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009044 - xylan 1,4-beta-xylosidase
                     activity [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HEV9036755.1"
                     /translation="MMKLLSISVLLFSISCFGQRLPYQDPALPSEDRAKDLISRLTLT
                     EKALLMQDQSPAIPRLGIKKFNWWSEALHGLANNDSVTVFPEPIGMAASFDDSLVYRV
                     FDAVADETRAKYNQAMSRGQENRRFLSLSVWTPNINIFRDPRWGRGQETYGEDPYLTS
                     RMGVSVVKGLQGPADGRYRKLLACAKHFAVHSGPEWSRHVLNVNDVSPRDLWETYLPA
                     FKSLVQDADVREVMCAYQRIDDEPCCGSNRLLQTILRDDWGFKYLVVSDCGAVTDFYT
                     SHKVSSDAVHASAKAVSAGTDVECVWAGYAFAHLPEAVARGLITEKEIDKHLLRVLVG
                     RMDLGDFDDDALVPWTKIPATVINDPEHRELAAKMARESMTLLQNRNNILPLNPGVRR
                     LAVIGPNADNEQMLWGNYNGKPLRTITILDGIRSKVNARAVVYDKACDLVDNKVTISY
                     MSKTSGEGRPGFTAKYWNNRDMKGEVAATGRITAPLKMTTAGDHEFASGVKLEGFSAS
                     YETEFEAQATEEIVFKYGATGHFELLVDGVSLARSNNWRTLPSRVPFKVEKGKKYKLE
                     IRYAQLNDWQANLEFDFGKEVDIDYSSLIDRLKGVDVVIFAGGLSTLLEGEEMPVNYP
                     GFKGGDRTDIELPEVQRNCLKALKAAGKKVIFVNCSGSAIALTPVTDDCDAILQAWYG
                     GESGGQAVADVLFGDYDPSGKLPVTFYKSSAQLKDFESYSMHGRTYRFMDDALFPFGF
                     GLSYTSFSIGEADAGNTVVTKESGLSLAVPISNTGKRTGTEVIQVYVRRANDSSGLLK
                     TLRGFSRVTVPAGKTVTAEIRLPYGSFEFYNDQQLQLAVTPGDYEVWYGTSSADRDLK
                     RVNVTVR"
     gene            complement(3285..5207)
                     /locus_tag="VGQ51_09050"
     CDS             complement(3285..5207)
                     /locus_tag="VGQ51_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV9036756.1"
                     /translation="MRTSIPFLVVCCLLIHRTLHAQGPEQHAPAGFDSVRTDIPHGKV
                     DTIVYPSKTVGTNRKATIYFPPGYSRKKQYPVLYLLHGIGGDEKEWLNGGHPQVILDN
                     LYAADKLVPMIVVMPNGRAMKDDRATGNIMAADKIQAFANFERDLLDDLIPFIEKKFK
                     VYTDREHRAIAGLSMGGGQSLNFGLGNLDRFAWIGGFSSAPNTKPPVQLVPDPDRARS
                     LLKILWISCGDNDGLMPFSSRTHEYLLAHGVPHIFYVEPGVHDFKVWKNGLYMFSQLL
                     FKPVDASLFSRYSPLGLPASTNVRNARYPQIMPDHRVLFRVRAPEAQRVQVDLVRKYN
                     LQKDTGGYWTGLTDSVSEGFHYYSLLIDGVPLADPASETFYGMGRMASGIEIPYAGGD
                     YYATREVAHGEIRIRSYFSMVTRSWRQLYVYTPPGYDSAIAEHYPVLYILHGGGEDET
                     GWAMQGRTNFIIDNLIAEKKAVPMIVVMPDANMPAPAFDETGLKQFESEMKQAIIPFV
                     EHHFRVQADAGHRAMAGLSMGGIHTLYTGVRNYPLFGYLGVFSSGWIVPRQQAIADDQ
                     YAFLREHAAAVNDSLKVFWFSEGGKEDIAYANGQKMLAKMDELKIHHIYYEYPGGHTW
                     PVWRNDLYHFAQLLFK"
     gene            complement(5224..>7013)
                     /locus_tag="VGQ51_09055"
     CDS             complement(5224..>7013)
                     /locus_tag="VGQ51_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEV9036757.1"
                     /translation="VGDDFYHCGSSFHFTPYLPIYHSKDLVHWEVIGRVVSPSMADSF
                     VTDRPSGGIWQGAITYFYGSYWIYFSANGQWFCKASSPAGPWSAPVRVRTNRTTGDLG
                     YDNSIFVDDDGKPYMLIKNGQKINRIQTLGRDGQLTDTVINLDWINARLQYSWAEGPV
                     MCKHNGYYYYFPAGDVSGGQYVLRTRRLTSDSTAWERLGDFFEPVTEDGVGLARPNHI
                     AAPVQLADGTWWTLGHSYENYGHDDWSGMGRQTSLYRVIWKDDRPWGLAPTTRPMIKP
                     DLPRSGILWASARGDDFNRTLLGLQWHFLSRKAAASYSLSDRKGWLRLTPGTGRTHLV
                     QKETDHYYSAVTKVDFDAADTASGAGIYLTNGNQQVVVQLYTGYDHGRRIILQLDTAV
                     RSVANGAGKVVWLRLERRWHELTGYYSKDGKAWVSLGAPISAVALDKAQPNWNSWVGT
                     SMGMFAEGKPADFDWFRCKDAFSPLLALGHANEYGIMTVIHEGEKSVTNTSPGGWFMI
                     PGVETGEDAPHGVELSVAATAAGDIQIWLDDLQDGKMVATVPVEATGGEWKTVSAKVA
                     NFEGHHDVFVKYPVEGQAKIFIRELRFTK"
BASE COUNT         1562 a         2082 c         1795 g         1574 t
ORIGIN      
        1 ctcaccggat aactattata actacatggg tgacggcgcc gtggatttcg atgaaaagat
       61 aagtttggta gccgcccaga tccgcgaggc aagggtaaag atggggttca aaaatcccat
      121 ttatctttct gtcgacgagt acggcgcctt tgccaggaat ttcatgtccg tcctaccggt
      181 cgcacagtgt ctcaattcct ttatccggca tgccgatgtc gtcaggatgg ccaatttcac
      241 catgctcact tcactgttat cgagcgaccc ccggaacggc acgttcaggt cgccggtgtt
      301 ttacacgttc caactattct ccaataactg tctcggggca tccgtcgatg cctatgttgc
      361 ctgcgatacg tttggaacgg aaaaacacaa gggcattcct tacctcgacg ttaccgcggt
      421 atatagcaag gtgaacggcg tcctgtacat taatgtggtc aaccgtcacc agtccagacc
      481 cattgctgcg gatatatccg ccgcctcggg taatttttca ggaaaggcgg aggccagcat
      541 catcaacagc gacgatctga aagcggcttt cacctatgaa catcgctcac agtatatgcc
      601 cgttgcagcc cccgtgaacg tgaacggcaa caaactgcaa tacacctttc ccccgcattc
      661 cttcacacaa ataaaagtaa ccataaaata aacaggtcac ctcactgtaa cattgaccct
      721 cttcaggtcg cggtccgccg aactggtccc ataccatacc tcgtagtccc cgggagtgac
      781 cgcgagctgc agttgttgat cgttatagaa ttcaaacgag ccgtaaggaa gcctgatctc
      841 cgccgtcacc gtctttccgg ccggcaccgt cacccggctg aatccgcgca gcgtcttcaa
      901 caacccactg ctgtcgttcg cccgccggac atacacctgg atgacttcgg tgcctgttct
      961 tttgcccgtg ttggatatag ggacggcaag cgaaagcccg ctctccttcg ttacgacggt
     1021 attgccggca tcggcctctc cgatggaaaa gctggtatag ctgagcccga atccaaacgg
     1081 gaaaagcgca tcgtccataa accgataagt ccggccgtgc atcgaatagc tttcaaaatc
     1141 cttcaactgt gcggagctct tataaaaggt gaccggcaac ttgcccgagg gatcgtagtc
     1201 gccgaaaaga acatctgcca cggcctgacc gcccgactca cctccgtacc aggcctgaag
     1261 gatcgcgtcg cagtcgtccg tcaccggcgt caatgcaatg gccgacccgg aacaattcac
     1321 gaagatgacc tttttaccgg cagcctttaa tgccttcaga caattgcgct ggacctcggg
     1381 cagctcgata tccgttcggt cgccgccctt gaagccggga tagtttacgg gcatctcttc
     1441 tccttcgagc aaagtggaca ggccacccgc aaatatcacg acatccaccc ctttcagcct
     1501 gtcgatcagg gaggagtaat cgatgtcgac ctcttttcca aagtcgaatt ccaaattagc
     1561 ctgccaatcg ttcagctggg cataccggat ctccagctta tactttttac ctttttcgac
     1621 tttaaaggga actctcgacg gcagggttcg ccaattattg gatctggcga gcgagacacc
     1681 gtcgacgagc agttcaaaat gaccggtggc gccgtatttg aatacgatct cctccgtcgc
     1741 ctgcgcttcg aattccgtct catagctcgc ggagaagccc tcgagcttaa cacccgatgc
     1801 aaattcatgg tctcctgccg tcgtcatctt caacggagcg gtgatccggc cggtggctgc
     1861 cacctcgcct ttcatgtccc gattgttcca atacttcgca gtaaacccgg gccgtccttc
     1921 gccggatgtc tttgacatat agcttatcgt caccttgttg tccacgagat cgcaggcctt
     1981 gtcatacacc accgcccggg cattgacctt agaccgaatg ccgtccagga tcgtaatcgt
     2041 ccgcagcggt ttgccgttgt agttgcccca aagcatctgc tcattgtccg cattgggacc
     2101 gatgacggcc aggcggcgca cccccgggtt caggggaagg atattattcc tgttctgcaa
     2161 caacgtcatc gactcgcggg ccatcttagc cgccagttcc cggtgctccg gatcattgat
     2221 caccgtggcc ggtattttcg tccagggcac cagcgcatcg tcgtcgaaat cgcccaggtc
     2281 catgcgaccc accaataccc gcaacaagtg cttgtcgatc tccttttccg tgatcaatcc
     2341 ccttgccacc gcctcgggga gatgagcgaa cgcgtagccc gcccatacgc attccacgtc
     2401 cgtaccggcg gaaaccgctt ttgccgaagc atgcacggca tcggaggaca ctttatgact
     2461 ggtataaaaa tcagtgacgg cgccgcaatc cgaaacgacg aggtatttga agccccaatc
     2521 gtcccggagg atggtttgca ggagccggtt gcttccgcaa cagggttcgt cgtctatccg
     2581 ctgatacgcg cacatcactt cgcggacgtc tgcatcctgg acgagcgatt tgaaggcggg
     2641 aagatacgtc tcccacagat cgcggggact cacgtcattt acattcagca catgacggct
     2701 ccactcggga cccgaatgca ccgcaaaatg tttggcgcag gcaaggagct tgcgatacct
     2761 gccatcggcg ggtccctgca atccttttac cacggacacg cccatccgtg aggtaagata
     2821 agggtcttcg ccgtacgtct cctggccacg gccccagcgg gggtcgcgga agatattgat
     2881 atttggtgtc cacaccgaaa ggcttaggaa acgccggttc tcctggcccc tgctcatcgc
     2941 ctggttgtat ttggcgcggg tctcgtccgc gacggcatcg aacactcggt agaccaggga
     3001 atcgtcgaac gatgctgcca tgcctatcgg ttcggggaac acggtgacgc tatcgttatt
     3061 ggccagtcca tgcaaagcct cgctccacca gttgaacttt ttaattccca gccgcgggat
     3121 ggcgggagac tggtcctgca tcaataaggc cttttccgtc agtgtaagac gggagatcag
     3181 atccttcgcc ctgtcttccg agggaagggc cggatcctgg tagggcagtc gctgtccaaa
     3241 acagctgatg gaaaataaca ggactgatat cgatagtagc ttcatcattt aaaaagcaat
     3301 tgtgcgaaat ggtacagatc gttgcgccat accggccagg tgtggccgcc gggatattca
     3361 taataaatat gatggatctt gagttcgtcc attttggcca gcatcttctg cccgttggca
     3421 taggcaatat cttctttacc gccctccgag aaccagaata ctttcaagct gtcgttgact
     3481 gccgccgcgt gttcccggag aaaggcatat tgatcatccg caatggcctg ctgccggggt
     3541 acgatccaac ccgagctgaa cacgccgagg tacccgaata acgggtaatt gcgcacgccc
     3601 gtataaagtg tatggatgcc gcccatggac aagccggcca tggcccggtg accggcgtca
     3661 gcctgtaccc gaaaatggtg ttcgacgaac ggaatgatcg cctgtttcat ttccgactcg
     3721 aactgtttga ggccggtctc gtcgaaagcg ggggcaggca tattggcatc cggcatcacg
     3781 acgatcatcg gtacggcctt cttttccgca atcaggttat cgatgataaa gttcgtccgt
     3841 ccctgcatag cccatcccgt ctcatcctcg ccgccgccat gcaggatata aaggaccggg
     3901 tagtgctcag ctatcgcgga atcgtacccc gggggcgtat agacgtatag ctgccgccag
     3961 gatcgggtca ccatcgagaa ataggaacgg atccgtatct ccccgtgcgc aacttccctg
     4021 gtcgcatagt agtcgccacc cgcatagggt atctctatcc cgctcgccat ccggcccatg
     4081 ccatagaagg tttcgcttgc cggatctgcc agcggcaccc catcgatgag caaggaataa
     4141 tagtgaaatc cttcgctcac cgaatccgtc aggccggtcc aataaccgcc ggtgtccttt
     4201 tgcaagttat atttccgcac gaggtcaacc tggacacgtt gcgcttcggg agcgcgcacg
     4261 cggaacaaca cgcgatggtc cggcatgatc tgggggtatc ttgcattgcg gacgttagtc
     4321 gatgccggta gtcccagagg actatagcgt gaaaacagcg aagcatccac cggtttaaag
     4381 agcaattgag agaacatata caggccgttc ttccatacct tgaaatcatg cacgccgggt
     4441 tccacataga agatatgcgg tacgccatgg gccagcaaat actcatgcgt cctgctggaa
     4501 aacggcatga gcccgtcatt atccccacaa gagatccaca ggatcttcag gagagatctt
     4561 gcccggtcgg gatccggcac cagctgcacc gggggcttcg tattgggggc ggatgagaat
     4621 cccccgatcc aggcaaaccg atcgaggttg cccagtccga aattcagcga ctggcctccg
     4681 cccatcgaca gtccggcgat cgcgcggtgt tcgcggtcgg tgtatacctt gaactttttt
     4741 tcgataaacg gaatgaggtc atccaaaagg tctcgctcga agttggcgaa ggcctgtatc
     4801 ttgtcggccg ccatgatgtt ccctgtcgcc cggtcgtctt tcatggcccg gccgttgggc
     4861 atgacaacga tcatcggcac gagcttatct gcggcataga gattatcgag gatcacctgc
     4921 gggtgaccgc cattcagcca ttccttttca tcgccgccga taccgtggag caggtacagg
     4981 acaggatatt gtttcttccg tgaatagccg ggcgggaaat agatggtcgc tttcctgttg
     5041 gtcccgacgg tcttcgaggg gtatacgatc gtatcgactt ttccatgcgg tatatccgta
     5101 cgaacagaat cgaagccggc cggcgcatgc tgctcagggc cctgggcgtg cagggtccga
     5161 tgtatcaaca gacagcagac aacgaggaat gggatggatg ttctcattgt atcgtgagtt
     5221 ttattatttg gtaaaccgta gctcccggat aaagatcttt gcttgcccct ccaccgggta
     5281 tttgacaaac acatcgtgat ggccttcgaa attcgccacc tttgcagaaa cggtcttcca
     5341 ttcaccgcct gtcgcttcca caggcaccgt ggccaccatt ttgccatcct gcagatcgtc
     5401 aagccagatc tggatatcgc ccgccgccgt agccgcgaca ctcaattcga cgccatgggg
     5461 ggcgtcttca cccgtctcaa ctcccgggat catgaaccat ccgcccggcg aagtattcgt
     5521 cacgctcttt tcaccttcgt gaatcacggt catgattcca tattcgttgg catgaccgag
     5581 cgccaacagg ggagaaaagg catctttgca tctgaaccaa tcgaaatccg ccggcttgcc
     5641 ttccgcgaac atacccatgc tcgtacccac ccaggaattc caattcggtt gagccttatc
     5701 taatgccaca gcgctgatcg gcgcccccag tgaaacccac gccttaccat ccttgctata
     5761 gtacccggtc aattcgtgcc atcttctttc caatcggagc cacaccacct tccccgcccc
     5821 attcgccacg ctacgcacag cggtatccag ctgcaaaatg atccggcggc catgatcata
     5881 gcctgtatac agctgcacaa ccacctgctg gttcccgtta gtcaaataaa tgcccgctcc
     5941 ggacgccgta tcggcggcat cgaagtctac cttcgtcacg gcggaatagt agtgatcggt
     6001 ctccttctga acgagatgcg tacgcccggt ccccggtgtc aatcgtaacc agcccttcct
     6061 gtccgacagt gaatagcttg cggcagcctt gcgcgacagg aaatgccatt gcaatcccaa
     6121 tagcgtccta ttaaaatcgt caccccgcgc gctggcccat aagataccgg atcggggaag
     6181 atccggcttt atcattggtc gggtagtcgg cgccagtccc cacggacgat cgtctttcca
     6241 gatgacccgg tatagcgagg tttgccggcc catgcctgac caatcgtcat gaccataatt
     6301 ttcataacta tgacccaatg tccaccaggt gccgtcggcc agctggacgg gtgctgcaat
     6361 atgattgggt cgcgccaggc ctacgccgtc ctctgtgacg ggttcaaaaa aatcacctag
     6421 cctttcccag gccgtcgaat cgctggtcag ccgccgggtc cgcaacacgt actgcccgcc
     6481 ggatacgtcg ccggccggaa aataatagta atatccgttg tgcttgcaca tcacgggtcc
     6541 ctccgcccag ctatattgca atcgggcatt gatccagtcg aggttgatga ccgtatcggt
     6601 cagctgacca tccctgccca atgtctggat acggttgatc ttttgaccgt ttttgatcag
     6661 catgtaaggc ttgccgtcat cgtcgacgaa aatcgaattg tcatagccta aatcgcctgt
     6721 ggtgcggttt gtccgcacac gcacgggcgc cgaccagggc cccgcgggcg agcttgcctt
     6781 gcaaaaccac tgaccgttcg cagagaaata gatccaatac gaaccataga aataagtgat
     6841 agccccctgc caaataccac ccgagggccg atccgtcacg aaagagtcgg ccatggaagg
     6901 cgataccacc cgaccgatca cttcccaatg gacgagatct ttggagtgat agatgggaag
     6961 ataaggggtg aaatgaaagg acgatccgca atgataaaaa tcgtcgccta cct
//
LOCUS       DASYSV010000304         8056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2362852, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000304 DASYSV010000000
VERSION     DASYSV010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2362852"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VGQ51_09060"
     CDS             <1..658
                     /locus_tag="VGQ51_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEV9036758.1"
                     /translation="TQGDYAQKKVYNEHPLEVAQAFEDAGLRRLHLVDLDGARAGAVR
                     NWKVLEGIAGKTNLIIDFGGGIKTNADVDIVFNSGAAMATVGSVAVKDAALFVSWLGE
                     YGADRFLLGADVRKEKIAVSGWTETTDRWVYDFIREYMEKGVRQVFCTDVSKDGLLQG
                     PALDLYKNIVGKFPELHFIASGGVSGMDDVRRLEEVGCRGVIIGKAIYEGRIKLKELC
                     "
     gene            652..1410
                     /gene="hisF"
                     /locus_tag="VGQ51_09065"
     CDS             652..1410
                     /gene="hisF"
                     /locus_tag="VGQ51_09065"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEV9036759.1"
                     /translation="MLTKRIIPCLDIRDGRTVKGVNFENIRDAGDPVELGALYAREGA
                     DELVFLDITATNEKRKTLSELVNRIAHHINIPFTVGGGISGVEDVSVLLKNGADKISV
                     NTSAFRRPELISELAKEFGSQCVVLAIDTRMEEDGNWYVYLNGGRVRTSTLCFEWARE
                     GVDRGAGEILLTSMNNDGTKKGFALDITRRLAEGLPVPVIASGGGGVPLHFAEVLGPG
                     KADAALAASIFHFKEVGIPELKGYLQKRGVAVRL"
     gene            1422..2030
                     /gene="hisIE"
                     /locus_tag="VGQ51_09070"
     CDS             1422..2030
                     /gene="hisIE"
                     /locus_tag="VGQ51_09070"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEV9036760.1"
                     /translation="METLKVNFSKSPDGLVPAIIQDCATEKVLMLGYMNQDALDRTLA
                     EGKVTFFSRGKQRLWTKGEESGNFLLVRHIALDCDQDTLLVKAEPLGPVCHTGADTCW
                     DEKNHREDFLVCLEEIIEQRKREARPETSHIARLFSKGINKIAQKVGEEAVELVIEAK
                     DHDDGLFLNEAADLLFHYLILLNAKGFTLQDVTNVLKERHKK"
     gene            2035..3144
                     /locus_tag="VGQ51_09075"
     CDS             2035..3144
                     /locus_tag="VGQ51_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV9036761.1"
                     /translation="MRKIIIVVLMAGPVMGWAQSKTGAAARFSITGDVTGLPEGSEVF
                     LTDPSNPGDTLATTHARAAGKFELNGHVDETNLYELNFDAARKKAPLFMGNDKMQITG
                     TMNDLGHLKTSGSPANDDFVEFQARFTPYFTRLNTLISEANSQGHMDKNGELFQHYKK
                     LTDTIFTVVDGFIAEKRASYVAPFVLVVVNQLTQDVAAQVRRLHSLAADVQKSYYGQY
                     LQKALDDALVGAVGSDEIDFTQNDTAGRAVSLSSFKGKYVLVDFWASWCGPCRMENPN
                     VVATYQKFRNKNFTVLGVSLDKAREPWLRAIKDDGLLWTQVSDLKFWYNEAAVKYHIA
                     SIPSNFLVGPDGKIVGRDLRGAALQEKLCELLGCN"
     gene            complement(3329..4447)
                     /locus_tag="VGQ51_09080"
     CDS             complement(3329..4447)
                     /locus_tag="VGQ51_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEV9036762.1"
                     /translation="MRKVLTAGILPALLCLVSTLPAKAQLTTMPDGGNKMATVSERIG
                     ITDVTIHYNRPGVKGREGTIWGKLIPAGYNDLGFGTSKAAPWRAGANENTTIQFSTDV
                     TVEGQSLPAGKYGFFVAYDPNECTLIFSRNSTSWGSFFYDPKEDVLQVKVKPVAMDKN
                     VEWLKYEFTDEKDDAATVALEWEKLSIPFRVAVDYPKTQIASFRRELRSDKGFTWNSY
                     MQAAQFCAQHNTDLDEGLVWADKSISEPFIGDKNFQTLSTKAQILTLMNRQTEADALM
                     KEALPLGKEAEVHQYARILLKAKKNKEAFEAFKLNYDKHPNEFTTNMGMARGYSGMGD
                     YKKALDYLTKAEAQAPDKANKDNIARLKPMLQEGKDIN"
     gene            complement(4642..5358)
                     /locus_tag="VGQ51_09085"
     CDS             complement(4642..5358)
                     /locus_tag="VGQ51_09085"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEV9036763.1"
                     /translation="MTKLSVNINKFATLRNSRGGNNPDVVKAAIDAQEFGADGVTVHP
                     RPDQRHIRYADVYAIKKIITTEFNIEGNSQEQKFVDLVLDVKPTQVTLVPDALGQITS
                     NHGWDTIRHKAYLVDIISIFKKAGIRVSLFVDPVIAMIEGAADTGTDRIELYTEAYAH
                     AYAADPASAIAPYHKAALRAHELGLGLNAGHDLDLQNLRYFAHNIPELDEVSIGHALI
                     CDSLYLGFENTIQLYKRQLQ"
     gene            complement(5484..5780)
                     /locus_tag="VGQ51_09090"
     CDS             complement(5484..5780)
                     /locus_tag="VGQ51_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036764.1"
                     /translation="MAIEISVYPNPFDHYIMLEITCDENIDCIVTLADIGQGKIIRML
                     GVSLVGGTNRIPFDNLESLSAGNYQLEIKTSAGEPVYRTRLFKQSVEGPVIHLN"
     gene            complement(5816..5938)
                     /locus_tag="VGQ51_09095"
     CDS             complement(5816..5938)
                     /locus_tag="VGQ51_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036765.1"
                     /translation="MKTMINFLNALVNTLTFSKEQMPALIPVRVVTKVHPDQHR"
     gene            complement(6321..>8056)
                     /locus_tag="VGQ51_09100"
     CDS             complement(6321..>8056)
                     /locus_tag="VGQ51_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9036766.1"
                     /translation="LEGTAGKNFSWLIGLRNRTNGSLLSSQETKGTYAPSSSDIQTQL
                     SWRLNGTSQLELLGILSQTRFNFIPQFSQLTSSIFSPYYTANLGLDTYFEGQEKSRYQ
                     TNMLGLSLSQQLNPHLRLKWMASRFEDKEQQNYDITGAYLFGDRDFDKSSPTFGQINN
                     PLGAGTYQNFARDNLDITVWNATHKGYLTLDRHYIQWGVSAERQLVDDHLHEWRYQDS
                     AGYSLPNTPGIPSITGVANGSDDPGITRLTGYAQDNLLFSDSGSFTLQGGVRYNYNTL
                     NKEFLVSPRLGASWKPRNSTKDIIYKAAIGIYDQPPFFREMLRPDGSLNKDLKAQRSW
                     QAVIGFDKNFHIGDRPFRFTTEAYYKNMTRVNPYDIDNVKIQYFALNDAKAYATGVEF
                     RLFGQLVKDADSWVSMGFMRSMEKIDTFFYNYTLDSLGHPLDSARTPMGWVRRPTDRL
                     FTFGLFLQDYLATNKNLKAYLNLLYGSNLPFSIPGNLHYRDALTIDPYIRIDVGFSAL
                     LLDSEKGHRRSHNPFRSFDNIWATLEIFNLIDRANTISYMLIKDFSNTTYAIPERLTP
                     RLINLKLIGRF"
BASE COUNT         1728 a         1671 c         2765 g         1892 t
ORIGIN      
        1 gacgcaaggc gattacgcgc aaaagaaggt ttataacgag catccgttgg aagtggcgca
       61 ggcttttgaa gatgcgggtc ttcggcggct gcacctggtc gacctggacg gggcccgggc
      121 tggcgccgta cggaactgga aggtattgga agggatcgcc gggaagacga atctgatcat
      181 cgatttcggc ggaggcatca agacgaatgc ggacgtggac atcgtcttca attcaggagc
      241 cgccatggcg acggtgggta gtgtggctgt gaaggatgcg gcattgtttg tttcatggtt
      301 gggggaatat ggcgccgatc ggtttttatt gggagccgac gtgcggaagg agaagattgc
      361 cgtcagcggg tggacggaga cgacggaccg gtgggtctat gattttatcc gggagtatat
      421 ggagaagggc gtgcgacagg tgttttgtac ggatgtgagc aaagacgggt tgctgcaggg
      481 gccggcgctg gatctgtata aaaatattgt agggaaattt ccggagctgc atttcattgc
      541 cagcggaggg gtcagcggga tggatgatgt gcggcgcctg gaggaggttg ggtgtcgcgg
      601 ggtgatcata gggaaagcca tttatgaggg aaggataaaa ttgaaggagc tatgttgacg
      661 aagcgtatca tcccctgtct ggatatccgg gatggccgga cggtgaaggg tgtcaatttc
      721 gaaaatatcc gcgatgcggg cgacccggtg gagctggggg cgttgtatgc gagggagggc
      781 gcggatgagc tggtttttct tgatatcacg gctaccaatg agaagcggaa gaccctgagc
      841 gagctggtga accgcatcgc gcatcacatc aatatccctt ttacggtggg tggcggtata
      901 tccggtgtgg aggatgtcag tgtgctgttg aagaatggcg ccgataagat ctccgtgaat
      961 acgtcggctt tccggcggcc ggaattgata tcggagctgg cgaaggaatt cgggagtcag
     1021 tgtgtcgtgc tggctatcga tacgcggatg gaggaggatg gtaactggta tgtctatttg
     1081 aatggtggaa gggtgcgaac gtcgactttg tgttttgaat gggcgcggga aggtgtggac
     1141 aggggggcgg gggagatcct gctgacctcg atgaacaatg acgggacgaa aaagggcttt
     1201 gcgttggata ttacgcgtcg gctggcggag ggcttgccgg ttccggtgat cgcttcgggt
     1261 ggaggagggg tgcctttgca tttcgcggaa gtgttgggtc cgggaaaggc cgatgcggcc
     1321 ctggcagcca gcatcttcca tttcaaggag gtggggattc cggaattaaa aggatatttg
     1381 cagaagcgcg gggttgccgt gcgtttgtaa tggaaaacga tatggaaaca ttgaaggtga
     1441 atttcagcaa gtccccggat gggttggtgc ctgccattat ccaggactgt gcgacggaaa
     1501 aggtgctgat gctgggttat atgaaccagg atgcattgga caggactctg gcggaaggga
     1561 aggtgacctt tttcagccgg gggaagcagc ggctatggac caaaggcgag gaaagcggta
     1621 atttcctgtt ggtgagacat attgcgctgg attgcgacca ggatacgctg ttggtaaagg
     1681 ccgagccatt ggggcctgtc tgtcatacgg gggcggacac gtgttgggac gaaaagaatc
     1741 acagagagga ctttttggtt tgcctcgagg agatcatcga gcagcggaaa cgggaagccc
     1801 gtccggagac atcgcatatc gcgaggctat tcagcaaagg gatcaacaag atcgcgcaga
     1861 aggtgggcga agaggcagtg gagctggtca tcgaagcgaa ggatcacgac gacgggttgt
     1921 tcctgaacga ggctgcggac ctgttgttcc attaccttat tttgttgaat gcaaaagggt
     1981 ttaccttgca ggatgtaacg aatgtattga aagagagaca taaaaaatga acttatgcgg
     2041 aagataataa ttgttgtttt gatggctggg ccggtgatgg gttgggcaca gtccaaaacg
     2101 ggtgcggcgg cgcgtttttc gatcacggga gacgtgacgg gattgccgga gggttcggag
     2161 gtttttttga cggacccttc caatccgggt gatacgctgg ctacgacgca tgccagggcg
     2221 gcggggaagt tcgagcttaa cggtcatgtg gatgagacca atctttatga gctgaatttc
     2281 gacgcggcca ggaaaaaggc accgttgttc atgggtaatg acaagatgca gatcacaggg
     2341 acgatgaatg acctggggca tttgaaaacg tcggggtcgc cggcgaatga cgatttcgtg
     2401 gaatttcagg cgcgattcac accgtatttc acgcggctga acaccctgat ctcggaagcg
     2461 aattcgcagg ggcatatgga taagaacgga gagctttttc agcattacaa aaagttgacc
     2521 gacacgatct ttacggttgt ggacggattc attgctgaga agcgcgcgtc ctatgtagca
     2581 ccgtttgtgc tcgtggtagt caatcagctg acgcaggatg tagcggcgca ggtcaggcgt
     2641 ctccattcgc tggctgcgga cgtacagaaa agttattatg ggcaatattt gcagaaggcg
     2701 ctggacgacg cgttggtcgg tgcggtcggg tccgacgaga tcgattttac gcagaacgat
     2761 actgcgggac gtgccgtgag cctttcttct ttcaagggca agtatgtgtt ggtggatttc
     2821 tgggccagct ggtgtgggcc gtgccgcatg gagaatccca atgtggtggc gacctatcag
     2881 aagttccgga ataagaattt tacggtgctg ggggtcagct tggacaaggc gcgggagccg
     2941 tggttgaggg ccatcaagga cgatggtttg ttgtggacgc aggtaagcga tctcaaattc
     3001 tggtataatg aggctgcggt gaagtatcat attgcgtcga taccttctaa ttttctggta
     3061 gggcccgatg ggaagatcgt gggccgcgat ctgcgcgggg cggcgttgca ggagaagctg
     3121 tgtgagttgc tggggtgtaa ttgatcgtga tatagaggcc gcgccgatga agatcggtta
     3181 tgacgcggcc cgtaaaaaga tcaggctttt agactgcgtc agggattgca gcggcagccg
     3241 ggagcaaagc gaggactata gcgcagagcc cgacccgcgc cgccggtcat tatcttgcgg
     3301 cgcgggggac gtccaaaaat atcttcgatc agttgatatc ttttccttcc tggagcatgg
     3361 gtttcaaacg ggcgatgttg tctttattcg ccttgtcggg ggcttgagcc tcggctttcg
     3421 tgagataatc gagggccttt ttgtagtcgc ccatgccgga atagccgcgg gccatgccca
     3481 tattcgtcgt gaattcgttg gggtgtttat cgtaattcag cttgaacgct tcgaaagctt
     3541 ccttgttctt ttttgctttg agcaggatgc gggcgtattg gtggacttcc gcctctttcc
     3601 cgagtggtaa ggcttccttc atgagcgcgt cggcttcggt ctgtctgttc atcagcgtca
     3661 ggatctgcgc tttggtggac agggtttgga aattcttgtc gccgatgaag ggctcactga
     3721 tggacttatc ggcccatacg agcccttcgt ccagatcggt attgtgctgg gcgcagaact
     3781 gtgcggcctg catataggaa ttccaggtga agcctttgtc gctgcgcaat tcgcggcgga
     3841 aggaagcgat ctgcgttttc ggatagtcga ccgccacgcg gaagggtatg ctgagctttt
     3901 cccattccag tgcgacggta gcggcgtcgt ccttttcatc ggtgaattcg tatttcagcc
     3961 attccacatt tttgtccatc gcgacgggtt tgaccttaac ctggagcaca tcttccttcg
     4021 gatcgtagaa aaagctaccc caggacgtcg agttacgcga gaagatgagc gtacattcat
     4081 tggggtcata agcgacgaag aaaccgtatt tcccggcagg aagcgattgt ccttcgacgg
     4141 tgacatcagt ggagaattga atggtcgtgt tttcattcgc gcccgcgcgc catggtgcag
     4201 ccttgctggt gccgaatccc aggtcattat agcctgcggg gatcagcttg ccccagatgg
     4261 tgccttcgcg tcctttgacg ccgggtctgt tgtaatgaat ggtgacatcg gtgatgccta
     4321 tgcgctcgga gaccgtggcc attttattgc cgccgtcggg catggtcgtc agttgcgcct
     4381 tcgccggcag cgtcgagaca agacaaagaa gggccggcag gataccggcg gtcagtactt
     4441 ttctcataag aatgcattta tggttgaatg atggaccaaa aatagggtgc gggggggtgg
     4501 ggtgagaagt gaatttacag ggaaggcgga ttaccgggat ataaagcggc ggggtagagg
     4561 tggggcgtgg aagcgttgaa gaaggcgggg tggtcgttga agagcagggt cagtcgtcag
     4621 cgatgcaagc gtcgcagggg tttattgcaa ctgccgtttg tacaattgga tcgtgttttc
     4681 gaagcccagg tacagggagt cgcagatgag ggcgtgaccg atggagacct catcgagttc
     4741 ggggatatta tgagcgaaat accggagatt ttgaagatcg aggtcatggc cggcgttgag
     4801 gccgagaccg agctcgtggg cgcggagggc agccttgtga tatggagcga tggcggaggc
     4861 ggggtcggcg gcgtatgcgt gggcataggc ttcggtatac agctcgatcc ggtcggtgcc
     4921 cgtatcggcg gcgccttcta tcatggcgat cacagggtcg acgaagaggc tgacccggat
     4981 gccggctttt ttgaagatgc tgatgatatc cacgaggtat gccttatggc ggatggtatc
     5041 ccagccatgg ttcgaggtga tctgacctaa cgcatccggg acgagggtga cctgggtggg
     5101 ttttacgtcc aggaccagat cgacgaattt ttgctcctgt gaattgcctt cgatattgaa
     5161 ctcggtggtt atgatctttt tgatggcata gacgtcggca taccgtatgt gccgttggtc
     5221 cgggcggggg tgcacggtga ctccgtcggc gccgaattcc tgggcgtcga tggcggcctt
     5281 aacgacatca gggttgttgc ccccgcggct gttgcggagg gtagcgaact tattgatgtt
     5341 gacggatagt ttagtcatgc ctcaaagtta gcgaaaaggg gggaggcagg gccgaaaaga
     5401 cgggtggtga gcgccgtatt gatgatgacg ggatccggga tcagtcgaag gtcgggtgaa
     5461 tatggccggg acctgcgccg ggatcagttg aggtggatga cagggccctc gacggattgt
     5521 ttgaaaagac gggtccggta gacgggttct cctgcggatg ttttgatctc cagttgatag
     5581 ttgccggcgg aaagggattc gaggttgtcg aaagggatgc ggttggtgcc gcccaccaaa
     5641 cttacaccca gcatgcggat gatcttaccc tggccgatgt ctgccagtgt aacgatacaa
     5701 tcgatgtttt cgtcgcaggt gatctccagc ataatgtaat gatcgaacgg gttcggataa
     5761 accgaaattt caattgccat gaccatagcc tcaaagttta ggggttatct tgaagttatc
     5821 tgtgttgatc gggatgtact ttggtaacta cccggaccgg tatcagtgcg ggcatttgtt
     5881 ctttggagaa ggtaagagtg tttaccaggg cattcaggaa gttaatcata gttttcatgg
     5941 tgcgcgattt acagtataag tgaccaattt gcggccaaaa attaaataac tgattataaa
     6001 tcaattgtag aggggcgggg aagatcgtat ttcccgaaat gggaagattg cggctttttt
     6061 gggaatggat gggaaagtta cgagggaaaa tacgggaatt ggggcaggtg gggatggcgc
     6121 ggagatgatg gaaagcggcg tccggagggc gggcgggcgg cgtcgtgcga aggttgggta
     6181 ggagcggcga gggggcggga gcggcgtggg cgggcggcga taggggcgag agcggggcgt
     6241 gggaggacgg gcggcgatgg gggacgggag cggggcgtgg gcgagcggcg agggaggacg
     6301 ggagcggggc gtgggcgcgg ttagaaccgg cctatcagct tgagattgat aagacggggg
     6361 gtgagccgtt cggggatggc gtaggtagtg ttggagaagt ctttgatcag catgtaagag
     6421 atggtattgg cgcggtcgat caggttgaag atctcgaggg tagcccagat gttgtcaaag
     6481 ctgcggaagg ggttatggct ccggcgatgg cccttttcgc tatcgaggag cagcgcgctg
     6541 aaaccgacat cgatgcggat ataggggtcg atggtcagag catcccgata atgcagattg
     6601 ccggggatgc tgaacgggag attgctgcca tacaggagat tcagataggc cttgaggttc
     6661 ttattggtgg cgaggtagtc ctggagaaac agaccgaagg tgaacagccg gtcggtcggg
     6721 cggcgtaccc agcccatggg cgtcctggcg ctgtcgaggg ggtggcccag cgagtcgagt
     6781 gtataattgt agaagaacgt gtcgatcttt tccatgctcc gcatgaagcc catgctgacc
     6841 cagctgtccg catccttcac tagctggccg aagaggcgga actcgacgcc ggtggcgtag
     6901 gcttttgcat cgttgagcgc gaaatactgg atcttgacat tatcgatatc ataggggttg
     6961 accctggtca tgtttttata ataggcttcg gtggtgaacc gaaagggtct gtcgccgatg
     7021 tgaaaattct tgtcgaaacc aatcactgcc tgccagcttc gctgggcttt gaggtcttta
     7081 ttcagcgagc cgtcggggcg caacatctcg cggaagaacg gcggctggtc gtagatgcct
     7141 atggccgctt tatagatgat gtcctttgta gaattgcgcg gtttccagga agcgcccagg
     7201 cggggggaga cgagaaattc tttgttgagc gtgttataat tgtatcgaac gccgccctga
     7261 agggtaaaag agccggaatc gctgaagagc aaattatcct gcgcataacc ggtcaggcgg
     7321 gtgatgccgg ggtcatcgct gccatttgcg acgccagtga tggaaggtat gccaggcgta
     7381 ttgggtaacg aatagccggc ggagtcctgg tatcgccatt cgtggagatg atcgtcgacg
     7441 agctggcgtt ccgcgctgac gccccattgt atatagtgac gatccagtgt gaggtagcct
     7501 ttatgtgtcg cattccatac cgtgatgtcg agattgtcgc gggcgaaatt ctgataagtt
     7561 ccggcgccca gcggattgtt gatctggccg aacgtggggc tgcttttatc gaaatcgcgg
     7621 tcgccgaaaa gataggcgcc ggtgatatcg tagttctgct gctccttgtc ttcgaaccgg
     7681 gaggccatcc atttcaaccg caaatgggga ttgagctgtt gcgataggga aagcccgagc
     7741 atattggtct ggtaccggct tttctcctgt ccttcgaaat aggtgtcgag accaaggttg
     7801 gctgtatagt aaggtgaaaa gatcgaggag gtgagctggc tgaactgcgg gatgaaatta
     7861 aaacgcgttt gcgagagaat gccgagcagc tccagctgcg aagtgccgtt cagccgccag
     7921 gatagctggg tctggatatc ggaggaggag ggggcatagg tgcccttagt ttcctgactg
     7981 ctgagcagag agccgttggt tcgattgcgg aggccaatga gccaggagaa atttttgccg
     8041 gccgtccctt cgagat
//
LOCUS       DASYSV010000305         8677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2490244, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000305 DASYSV010000000
VERSION     DASYSV010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490244"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..873
                     /locus_tag="VGQ51_09105"
     CDS             43..873
                     /locus_tag="VGQ51_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEV9036767.1"
                     /translation="MMSLRRTGILFLVSILFSCLRVAAQNDSQATDTTVADTVVVGAG
                     QGSSVTLRNDTAYVTGVDKAASAHGEDVVSDSLVARTVPDSVVRAWKSAPGFAYANDP
                     HYWKRVRQEDDPMPSWPSRLLSSSAFRYGIYIILGALLLYAIGRIVTENQLGIFYRGA
                     KRSGGSGTPVVDEPVMEEDLDRRLQQSMDTGDYPQAVRYSYLRTLRRLDERGLIRLNG
                     RATNQEYLRQLNGTAQEASFRFLTYAYEKVCYGQFGLSEDSFRRLHGYFTEFDKTLPG
                     "
     gene            870..2039
                     /locus_tag="VGQ51_09110"
     CDS             870..2039
                     /locus_tag="VGQ51_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036768.1"
                     /translation="MRRFIPLIPVIALLSSCATKQPQLNRRLSLWRKDDIPYGTQVAF
                     EALPDLFPDAEVSVNKTSLLSTASPYRRKAYIIICSRLIAEDEDVTWMMNFVGAGNQI
                     FISANHISESLLHNLSVHATTDEQFASEPDSLTLGVYDPMDTGYHSYSYPGDSYSNSV
                     TALDSQYTSILGRDAQGHPDFIRIRYKGGGAIFLHFAPLAFSNFFLLHKENMTYYQRA
                     LSYIPSSVKEVVWDDYFRYDRRSAGFPLFNFLSRNPPLRWGFWLLLLLFLIIYLFDSK
                     RRQRMVPLIVPPRNTSLDFVRTIGQLYYQRRDNHNLAMKMTVHFQEQVRTRYHMTATL
                     EEDGFVDRLAYRTGYPKSVLEWLVGYMRQLPLAAYVPDAQLMEFHRQLEAFYKHS"
     gene            2041..3018
                     /locus_tag="VGQ51_09115"
     CDS             2041..3018
                     /locus_tag="VGQ51_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV9036769.1"
                     /translation="MEDNLFEQRTDLDALDMAVQQLKTEIGKVIVGQEAMIELLLAGI
                     LADGHILIEGVPGVAKTLTAKLLSKVISVGFSRIQFTPDLMPSDVIGTSVFNPRDSNF
                     QFKHGPIFSNIVLIDEINRAPAKTQSALFEVMEERQITVDGQTYHLMPPFMVVATQNP
                     IEHEGTYHLPEAQLDRFLFKIKVGYPSQEEEFHIVSNHHGMKGEQRIETVGPVLTAAR
                     IQELRDKVRGLHVEEKLIRLITTIVNATRNHNSIFLGASPRASIGILNGSKALAAMRG
                     RDFVTPEDIVYITPAVLRHRLVLTPQSEMGGTTVDGVITEILQSIEIPR"
     gene            3021..4361
                     /locus_tag="VGQ51_09120"
     CDS             3021..4361
                     /locus_tag="VGQ51_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV9036770.1"
                     /translation="MLKRFYKSLFLARRWYWLFAGVIFLFVLSYGAEFLFRIAQLFLL
                     FFVVVTVLDYLVLFIRRNPVKVRRLLSESMSNGDSNPVHLRVDNGYPFPVRLRIIDEL
                     PDQLQERNFSLDADLKPGETRDLDYVLRPKERGEYVFHNINVFIRSPFGLLIRRKIVE
                     AEMTVRVLPSYQELKKFELLAASQNLADSGVKRIRKLGHSLEFEQIKEYVTGDDIRNL
                     NWKATARKGGQLMVNTFTDEKSQQIYCLIDKGRVMKMPFDGMTLLDYAINATLVLSRV
                     ALIRQDKAGLLTFSDQIATFLPAGRMSTQMSHILEILYNQKTHWQETDFEKAYALVRA
                     RITQRSLIVLFTNFESLAGLRRQLPYIRAIARKHLLMVVFFENTELQQIRDTEARDIE
                     DIYIKTIADKFAYEKRLMVKELNQHGIATILTTPQHLTVHAVNKYLEIKARQAI"
     gene            4376..5104
                     /gene="truB"
                     /locus_tag="VGQ51_09125"
     CDS             4376..5104
                     /gene="truB"
                     /locus_tag="VGQ51_09125"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV9036771.1"
                     /translation="MDEVNVFELGQVLLIDKPLDWTSFDVVRKLRGMVRTRKIGHTGT
                     LDPLATGLLILCTGKFTKRINEYMAREKEYTGTITLGARTPTYDLESEPCDVKEVGAV
                     TADTVRGVIGSGFVGEIMQVPPAHSAIKVGGKRVYELARKGKEVKLEPRPVVIKEFEI
                     TAFRLPELDFRVICSTGTYIRSLANDLGAALGCGGYLSALRRTRIGEFRVGDALTMAA
                     AEGYIKEKFGGSAGGAGSGVAQNG"
     gene            complement(5092..7500)
                     /locus_tag="VGQ51_09130"
     CDS             complement(5092..7500)
                     /locus_tag="VGQ51_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEV9036772.1"
                     /translation="MSNSSMFRRRQFTSSFPTLALLLLTLAGSIFSCTVPRHYQYGKP
                     FVFSVNIKVEGSLRKDQKKDLAQKLSNQLDDSLRPQIISIAGIYDRVMYPPVFDTANL
                     RRSIGFMVALLNANGYFMPVIRDTIRRDTVTPGKKHPERKEYRVAVNFIVDPGKQLLL
                     DSVGFNLSAPALQALALETSGQTLLKKGSPYSNQLLAAERERLAGVFRNNGYYYLSRE
                     DLKVVVDTVVSALIDPNLDPFQQAALLARLKQNREHPTINAVVEQRAVKDSSHLVKYH
                     IGHVTVYPDLPIILEDTITVSRIDTNAARGFSNISRADKFKTSTIINNIFVRPGNLFR
                     QQDYIRTINRFNQMGAWAQESMTFLPSDLSDSILDVTLRLYPNKKYFVNGDFEVARNT
                     NDIVTATNLFGINANLGLRNRNTFRESVLSSTTLRGGVEFGADFIQNTQASISHTISF
                     PQLLPKNLLRYLPRFILKRKSQLDSLHSVFNINASFVDRRQFFTMRSINGSFGYEWTH
                     GNKSWLIRPINIEFTQVDKTDSFNNYLLSNPSLNLAFKSGLVLSAQGVYKSLHKVGNH
                     TDFLSLSGESSGGLFGFIKTLDEGPLWRYIKGEVDYRHHIDYRHTQLAFHAYAGGGWA
                     YGREGSQWEQTLPFYKAFFAGGPNSMRGWQVRQLGLGSSKFYDTAGHASGTGPLDRFG
                     DIQLEGNVEWRFRIGSVFGFPLLSALYVDAGNIWNRHVLVDANQNTEEAMKGSDFKFD
                     RFYREFAVDAGTGLRIDFDLFIIRLDYAYKIKDPQRMDHSDTWFYDMRLFDGQFQLGI
                     GYPF"
     gene            7530..8291
                     /locus_tag="VGQ51_09135"
     CDS             7530..8291
                     /locus_tag="VGQ51_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV9036773.1"
                     /translation="MLITKTQVKYIQSLGHKKFRDAEGVFVVEGPKITEELLAVPRMR
                     PRAIYALPEWIQGLPAGLGKEVLTTVGAVELGRLSALTTPNQVIAVFEKPVFGDVESG
                     TGIHLVLDGIQDPGNLGTIVRLADWFGVASVLCTPDCADVYNAKAVQSTMGSIGRVPV
                     IYGDPAASIRGRAGVSVYATVLEGESVYGMGEIERGFIVVGNESRGIGPELLGLATRR
                     VRIPRVGGAESLNAAVATGIVLSHLSHMAAPKGTG"
     gene            complement(8275..>8677)
                     /locus_tag="VGQ51_09140"
     CDS             complement(8275..>8677)
                     /locus_tag="VGQ51_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9036774.1"
                     /translation="IVLIIMTVVATLNLVTCLIILVLERTHMIGILKSLGTTDWSIQR
                     IFLYEGSIITLFGIALGNVFGLSICWLQERYGFVTLPEEAYFISKAEVKLEWWHFLLV
                     DGGTFLICFLVLMIPTILVRRMKPARAIRFL"
BASE COUNT         1926 a         2139 c         2681 g         1931 t
ORIGIN      
        1 tatcgtttat cccatacgcc tggagaaaaa aatgcgatcg ctatgatgtc tctacgccgg
       61 acggggatcc tgttccttgt ttccatcctg ttctcctgtc ttcgcgtcgc cgcccagaac
      121 gacagccagg cgacggacac gacggtagcc gataccgtgg tagtgggggc cgggcaaggg
      181 agttccgtca ccctccggaa cgacacggcg tatgttacgg gcgtcgacaa ggcggcatcc
      241 gctcatggtg aggatgtggt gagcgattct ttggtcgcgc ggaccgttcc cgacagcgtc
      301 gtacgggcgt ggaaatcggc tccgggtttt gcttatgcca atgacccgca ttattggaag
      361 cgggtccggc aggaagacga ccccatgccg tcctggcctt cgcggttgtt atcgagcagc
      421 gcattccgtt atggtatcta tataatcctc ggggcgctgt tgctctatgc catcggcagg
      481 atcgtgaccg agaaccagtt gggtatcttc tatcgcgggg cgaaacggtc cggtggcagc
      541 ggaacccctg tggtcgatga gccggtgatg gaggaggacc ttgaccgccg gttgcaacaa
      601 tcgatggaca ccggggatta tccacaggct gtgcgttaca gctatctgcg aacattgagg
      661 cggttggatg agcggggttt gatccgcctg aacgggcggg cgaccaacca ggaatatttg
      721 cgtcagctga acgggactgc gcaggaagcg tctttccgtt ttttgaccta tgcctatgaa
      781 aaagtgtgtt acgggcagtt cggtctcagc gaggacagct tccggcgtct ccacggatat
      841 tttacggaat tcgataaaac cttacccgga tgagaaggtt catacccctg ataccggtga
      901 tcgcgctttt aagttcgtgc gcaacgaaac agccgcagct gaaccggcgt ctgtcgttgt
      961 ggcgcaagga cgatatccct tatgggacgc aggtggcttt cgaggccctg ccggatctct
     1021 tcccggacgc ggaagtgtcg gtcaacaaga ccagcctgct ttccactgca tcgccttaca
     1081 ggcgcaaagc ctatatcatt atttgttcgc ggttgatcgc cgaagacgag gatgtcacct
     1141 ggatgatgaa cttcgtgggt gcgggcaacc agatattcat atcggccaac cacatcagcg
     1201 aatcgctgct ccataatctg agcgttcatg cgaccacgga tgaacagttc gcatccgaac
     1261 cggatagcct gacactgggc gtgtacgatc ccatggacac cggttatcat tcttattcct
     1321 atccgggaga ttcctattcc aactcggtaa cagcgctgga ctcgcagtat acttcgatat
     1381 tgggacgcga cgcccagggc catccggatt tcattcgaat ccgctataag ggcggagggg
     1441 ccatctttct gcacttcgcg ccgctggcct tttccaattt ctttctgctg cacaaagaga
     1501 atatgaccta ttatcaacgc gcgttgtcat atatcccgtc ttcggtgaag gaggtggttt
     1561 gggatgatta tttccgttat gaccggagat cggcaggttt tccgctgttc aactttctct
     1621 ccaggaatcc cccgctgcgc tggggtttct ggctgttgct gctgctgttc ctgatcatct
     1681 atctcttcga cagcaaacgc cggcagcgga tggtcccgct gatcgtccca ccgcgcaata
     1741 catcgctgga tttcgtccgg accatcgggc agttgtatta tcagcggcgt gacaaccaca
     1801 acctggcgat gaaaatgacg gtgcatttcc aggagcaggt gcgaacccgc tatcatatga
     1861 ctgcgacgtt ggaggaggac ggattcgtcg atcggctggc ctaccggacg gggtatccaa
     1921 agtccgtgtt ggagtggctg gtggggtata tgcggcagct gccgttggcg gcctatgtgc
     1981 cggacgccca gctgatggaa ttccatcgtc aattggaagc attttataaa cattcataac
     2041 atggaagaca atctattcga acagagaacg gacctggatg cgctggatat ggcggtacaa
     2101 cagctgaaaa cggagatcgg caaggtcatc gtcggtcagg aagcgatgat agagctcctg
     2161 ctggcgggta tcctggcgga cggacatatc ctcatcgaag gggtgccggg tgtcgccaag
     2221 accctcacgg ccaaattgct cagcaaggtc atctctgtgg gtttttcacg tatacagttc
     2281 acccccgacc tgatgccgag cgacgtcata ggcacttccg tgttcaatcc gagggatagc
     2341 aatttccaat tcaagcatgg accgatcttc agcaatatcg tactcattga tgagatcaac
     2401 cgggcgcctg ccaagaccca gtctgctttg ttcgaagtca tggaggagcg gcagatcacc
     2461 gtggacggcc agacgtatca tctcatgccc cccttcatgg tcgtggccac ccaaaacccc
     2521 atcgagcatg aaggcaccta tcatcttccc gaggcgcaac tggacagatt cctgttcaag
     2581 atcaaggtcg gctatccttc ccaggaggag gaatttcata tcgtgtccaa tcaccacggg
     2641 atgaaaggcg agcagcggat cgaaacggta ggcccggtat tgactgccgc acggatacag
     2701 gaattgagag ataaggtccg tggtctgcat gtcgaggaga aactgatccg tcttatcact
     2761 accatcgtca atgccacacg gaatcacaat tccatcttcc tcggggcctc gccgcgggca
     2821 tccataggga tccttaacgg gtcgaaagcc cttgccgcca tgcgtgggcg ggacttcgtc
     2881 acccccgagg atatcgtcta tatcaccccc gccgtgttac ggcaccgtct cgtactgacg
     2941 ccgcaaagcg aaatgggcgg caccaccgta gacggagtca tcaccgagat cctgcagtcg
     3001 atagagatac cgagataacc atgctgaaaa gattctataa atccctgttc ctcgcccgcc
     3061 gctggtactg gctgtttgcc ggggtgatct ttttgttcgt gctttcctac ggggcggaat
     3121 tcctgttcag gatcgcgcag ttgtttttgt tattttttgt cgtggtaacg gtgttggact
     3181 acctcgtgct ttttatccgg aggaatcccg ttaaggtccg gcgactgctg tcggagagca
     3241 tgagcaacgg cgacagcaac cccgttcatc ttcgggtgga caacgggtat ccttttcccg
     3301 tcaggttgcg gatcatcgac gaattgcccg atcaattgca ggaaaggaat ttttccctcg
     3361 atgccgacct gaaaccggga gagacccgtg acctggatta tgtcctgcgg ccaaaagagc
     3421 ggggcgaata tgttttccat aacatcaacg tctttatcag gagtccattc gggttgctga
     3481 tccggcgcaa gatcgtagag gcggaaatga cggtaagggt tttaccttcg taccaggagc
     3541 tgaaaaaatt cgaattgctg gccgcatcgc aaaacctcgc cgacagcggg gtaaaacgga
     3601 tccgcaaact tgggcatagt ctcgaattcg aacagatcaa ggaatatgtc accggagacg
     3661 atatccgcaa tctcaactgg aaggcgacgg cgcggaaagg agggcagctg atggtgaaca
     3721 ccttcacaga cgaaaagagc cagcagatat attgcctgat cgacaagggc agggtgatga
     3781 agatgccctt cgacgggatg accttgctgg attatgccat caatgcgacg ctggtcttgt
     3841 cgcgtgtggc gcttatccgg caggataagg cgggattgct gacattttcc gatcagatcg
     3901 ctaccttttt accggccggc aggatgtcga cgcagatgag ccatatcctg gagatcctgt
     3961 ataaccagaa gacgcactgg caggagaccg atttcgaaaa ggcctatgcc ctcgtgaggg
     4021 cgcggatcac gcagcggagc ctgatcgtcc tgttcactaa tttcgaatcg ctggcggggc
     4081 tgcggcggca attgccgtat atccgggcca tcgcgcggaa gcatttgctg atggtggttt
     4141 tcttcgagaa taccgagcta cagcagatca gggatacgga agcccgcgac atcgaagata
     4201 tctatatcaa gaccatcgcc gataaatttg cctatgaaaa gaggttgatg gtcaaagagc
     4261 tgaatcagca tggtatcgca acgatcctga cgacgcctca acatctgacg gtacacgcgg
     4321 tgaataaata tttagagata aaagcgagac aggcgatata gctatatttg cgcccatgga
     4381 tgaagtaaat gtattcgagc tggggcaggt gttgctgatc gacaagccgc tggactggac
     4441 ctctttcgat gtcgtgcgca aattgcgcgg gatggtccgg acgcggaaga tcgggcatac
     4501 gggaacgctg gacccgctgg cgacgggtct gttgatcctc tgcacgggaa aatttaccaa
     4561 gcgtatcaat gaatatatgg cgcgggaaaa ggagtatacc ggaacgatca cgttgggtgc
     4621 gcgaacgcct acgtatgacc tggagagcga accgtgcgat gtgaaggaag tcggggcggt
     4681 gacggccgat accgtgcgcg gagtgatcgg gagcgggttt gtcggagaga tcatgcaggt
     4741 gccgcccgcg cattcggcta tcaaggtcgg gggtaagcgg gtgtacgagc tggcgcgcaa
     4801 gggcaaggag gtaaagctgg agccgcggcc ggtggtgatc aaggaattcg aaataacggc
     4861 attccggctg ccggagctgg acttcagggt gatttgttca acggggacgt atattcgaag
     4921 cctggccaat gacctgggag ctgcgctggg ttgcggagga tatttgagtg cgttgcgcag
     4981 gacgcggatc ggggagttca gggtcgggga tgcgttgacg atggcggcgg cggagggata
     5041 tatcaaagag aagtttggcg gatcggcggg aggggctggg agcggggtgg ctcagaacgg
     5101 gtaaccgatt ccgagctgga actgtccgtc gaaaagccgc atatcataga accatgtatc
     5161 gctatgatcc attcgctgcg ggtctttgat cttataggcg tagtccaggc ggatgatgaa
     5221 gagatcgaaa tcgatacgca gaccggtccc cgcatccacg gcgaactcgc ggtaaaagcg
     5281 atcgaacttg aaatcgctgc ctttcatggc ttcttcggtg ttctggttgg catcgaccag
     5341 tacatggcgg ttccagatat tcccggcatc cacatagagg gcgctgagca gcgggaagcc
     5401 gaacacgctg ccgatgcgga accgccattc gacatttcct tccagttgga tatctccgaa
     5461 ccggtctaac gggcccgtgc ctgacgcgtg tccggccgta tcatagaact tggatgagcc
     5521 gagacccagt tgtcgcacct gccacccgcg catactgttg ggacctcccg cgaaaaacgc
     5581 cttgtaaaag ggcagcgtct gttcccattg gctgccttcg cgtccatacg cccaaccccc
     5641 gccggcatag gcgtgaaagg caagctgggt gtgtctgtaa tcgatgtggt gccgatagtc
     5701 tacttcacct ttgatgtaac gccagagcgg gccttcatcg agggttttga tgaacccgaa
     5761 aagtccgccg ctggattcgc cgctgagcga gagaaaatcg gtatggttgc ccaccttgtg
     5821 caacgacttg tagacgccct gcgcactgag gaccaatccg ctcttgaagg ccagattgag
     5881 tgaagggttg ctgagcagat agttgttgaa actatcggtc ttgtcgacct gggtgaattc
     5941 gatattgatc gggcgaatga gccaggattt gttgccgtgt gtccattcat aaccgaagga
     6001 gccgttgatc gagcgcatcg taaagaactg gcggcggtcg acaaaggagg cattgatatt
     6061 gaataccgaa tggaggctgt cgagctggct ttttcttttg aggatgaatc tcggcaggta
     6121 acggagcagg tttttcggca ggagctgggg gaaggagatg gtatgcgaaa tactggcctg
     6181 agtgttctgg atgaaatcgg cgccgaattc cacgccgcca cgcagggtgg tcgaactgag
     6241 caccgactcc cggaacgtat tgcggttacg cagacccaga ttggcattaa taccgaaaag
     6301 gttggtagcg gtgacgatat cgttggtatt gcgggctacc tcgaaatcgc cgttgacgaa
     6361 atatttttta ttcggataca gcctgagtgt gacatcgagg atggaatcgg agagatcgga
     6421 gggcaggaag gtcatcgatt cctgcgccca ggcccccatc tggttaaagc ggtttatcgt
     6481 acggatatag tcctgttggc ggaagaggtt accgggccgg acgaagatat tgttgatgat
     6541 cgtggaggtt ttgaacttgt cagcccggga aatgttggaa aaacccctgg cggcgttggt
     6601 atctatcctg cttaccgtga tcgtatcttc gaggatgatc ggaagatcgg gatacacggt
     6661 gacgtgaccg atatggtatt tgaccaggtg gctggaatcc ttgacggccc gctgttccac
     6721 gaccgcattg atcgtggggt gttccctgtt ctgtttgagc cgggcgagga gagccgcctg
     6781 ctggaagggg tcgagattgg ggtcgatcag cgcggacacg accgtatcga cgacgacttt
     6841 cagatcctcg cgggaaagat agtaataacc gttgttgcgg aacacgccgg cgagccgttc
     6901 gcgttcggcc gccagcagct ggttggaata agggctgcct ttctttagga gcgtctgtcc
     6961 gctggtctcg agtgcaaggg cctggagtgc gggtgcggag agattgaaac ccaccgaatc
     7021 gagcagcagc tgtttgccgg ggtctacgat aaaattgacg gcgacgcggt attccttcct
     7081 ttcgggatgt ttcttccctg gtgtgaccgt atccctgcgt atggtatccc tgatgacggg
     7141 catgaagtag ccgttggcgt tgagcagggc gaccatgaaa ccgatggagc gccggaggtt
     7201 ggcggtgtcg aacacgggag gatacatgac ccggtcgtag atgcccgcta tcgagatgat
     7261 ctggggacgg aggctatcgt ccagctggtt ggacagtttt tgcgccagat ccttcttctg
     7321 gtctttgcgg aggctgcctt ctacttttat attgacggaa aagacgaagg gtttgccgta
     7381 ctggtagtgc cggggaacgg tgcaggagaa gatgctcccg gccagcgtga gcagcagcaa
     7441 tgcaagggta gggaaagatg atgtaaattg ccggcgccgg aacatagagg aattagacaa
     7501 cccagggaaa tttttttaaa cgaaatgcaa tgctgatcac taaaacgcag gtcaaatata
     7561 ttcaaagttt gggccataaa aaattccggg atgccgaggg ggtgttcgtg gtggaaggcc
     7621 ccaagatcac cgaagagctg ctggcggtgc cccgtatgcg tcccagggcg atctacgcgt
     7681 tgccggagtg gatacaggga ttgccggccg gattggggaa ggaggtgctc acaacggtgg
     7741 gggccgtcga gcttgggagg ctgtccgcgc tgacgacgcc caaccaggtg atagcggtgt
     7801 tcgagaagcc cgtattcggg gacgttgaat ccggcaccgg cattcacctg gtgctggacg
     7861 gtatccagga ccccggcaac ctgggcacga tcgttcggtt ggccgattgg ttcggggtcg
     7921 cttcggtgct ttgtactccg gactgcgccg acgtttataa tgcaaaggcg gttcaatcga
     7981 ccatgggcag tatcggacgg gtgcccgtga tctatggcga tccggctgca tcgatccggg
     8041 ggcgcgccgg ggtgtcggtg tatgccaccg tgctggaggg ggagagtgtg tatgggatgg
     8101 gcgagataga gaggggattt atagtggtag gaaatgaatc gagggggata gggcccgagt
     8161 tgctggggct ggctacccgg cgggtgagga taccgagggt tggcggcgcg gagtcgctga
     8221 atgcggcggt ggcgaccggg atcgtgttat ctcatttatc gcacatggcg gcgcctaaag
     8281 gaaccggata gcccgggcgg gtttcatcct gcggacgagg atagtgggga tcatgaggac
     8341 gaggaagcat atgaggaagg tacccccgtc gacaaggagg aaatgccacc attccagctt
     8401 tacctccgcc tttgagatga aataagcctc ttccggcaat gtgacgaagc cataccgttc
     8461 ctgtagccag cagatcgaca gaccgaagac gttaccgagc gcgataccga aaagggtaat
     8521 aatgcttcct tcatacagga agatgcgttg gatggaccaa tcggtggtgc cgagggattt
     8581 gaggataccg atcatatgtg tgcgttcgag cacgaggatg atgagacagg tgacgaggtt
     8641 cagggtagcg actaccgtca tgatgatgag aacgatg
//
LOCUS       DASYSV010000306         4515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1590971, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000306 DASYSV010000000
VERSION     DASYSV010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590971"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VGQ51_09145"
     CDS             complement(<1..256)
                     /locus_tag="VGQ51_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEV9036775.1"
                     /translation="MPNLYPGIKPLFPGGRRLACLCLVVFLPAIIATIVPTIVIAQPT
                     ISSVAPLRGPVGTPVTISGNNFNATPSSNIVYFGSVRAQVT"
     gene            365..1315
                     /locus_tag="VGQ51_09150"
     CDS             365..1315
                     /locus_tag="VGQ51_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV9036776.1"
                     /translation="MKPVTYYAAAVTAFTIWGFFSLVLRPLANYAALDILFYRVFISA
                     ALMFPVSSLIRRKVWVANRRLFSGLSHRDKRGLLFQLFGGGLFLTGNWFSYIYVVNNV
                     SVKASAFAYLVCPVLTTVFAWLILKEHLTRGQWLAVLLSAAGCGILAFNHLADLLYSI
                     VVAFSYALYLVSQRKDYGADRFLLLSGQVLFSALLLLALFPRFHGPAPQESRFYFLIV
                     VIAVAFTIFPLFLNLYALKGLRSSTVGILLNINPLVGFILAMTVFGEKVDVQQVAAYC
                     VIVVAVALFNLFNPVDRDGNRRRTPDVSIPDMSGVQSGGR"
     gene            1378..3768
                     /locus_tag="VGQ51_09155"
     CDS             1378..3768
                     /locus_tag="VGQ51_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9036777.1"
                     /translation="MFKNYWKIAWRNLVKDRQFSLLNLVGLSVGLACALLIGLWVMDE
                     LRMEKYNPNDDRLFQVMTNNKTSNGLQTGMYTPGILAKALRAEFPEVEDASSVLPASW
                     FNDPNTPGGVVSYDQKKLNATPQYVDSNYFHLFRCPILEGDRRRLFDGKQGVFLSSSL
                     AQTLFGTTHHLIGKVIHFDQYDFTGDFEIRGIFQPNPPNASEKPDLLFNFDVALERRP
                     GLQHWYNADPHTFVLLRPGADPRVLDSKLSHYLLAKQGGKGWDAQLFLAKFSDRYLYN
                     RYENGVQSGGRIVYVRLFIIIAVFILVIACINFMNLSTARATHRAREVGIKKVVGASR
                     IALILQYLGESVLLTCCALVAAVAICELLLPVFNGITGKQLSFHLAPNLLFLLAGVTL
                     LTGLFAGSYPAFYLSAFRPVAVLKGDIKTSWRELWARKGLVVFQFTLSILFIAGVLIV
                     YRQLSYIQSRDLGYNRDHVIDFNIPIKLDSIYLVHAASFVNELNNIPGVVSAGSHSHN
                     LTGDHGSISGVSWPGKDPGLDIEFANIEVGYHFLQTMGIRLKAGRYFSQNMAHEIVMN
                     ETAIKAMGLKDPLGKVVRFWDEKREIIGIAADFNFESLYQPVKPAFFRCYPVTNQVVV
                     RIMPGAEASTIAAVGKAYTRFNPGMDFEYRYLDEDYQRLYSSETRIGELSRYFAGLAI
                     LISCLGLFGLAAFSAARRRKELGIRKVIGASVVQLVYLMSKEFIWLVALAIAIAFPLA
                     WAGMHYWLNEFAYRTDITADLFWITGLAVILITLLTISYQSISTALANPIRSLRTE"
     gene            3779..>4515
                     /locus_tag="VGQ51_09160"
     CDS             3779..>4515
                     /locus_tag="VGQ51_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036778.1"
                     /translation="MLKNYLTIAWRHMVRYKGYAAINIIGLALGLVCCLFIWLWVQDE
                     RAVDNSHAAGSRLYLAYSTIISNGRVQGSYNTPVTYDYTANKPVFLTEDAGAVVPGVR
                     HVVFYRKGYELPWGHPESFQAGDKIIRLNGSRATANFFKVFSFPLIEGRPETALSDAG
                     SIAISRKMAIAFFGSPEKAIGRTLRYENRRNFSVTAVFEDIPAQSSLQFEFLLPWWSQ
                     GNGLAGLEYSTNNFDTFLELADNADTAA"
BASE COUNT          948 a         1163 c         1260 g         1144 t
ORIGIN      
        1 ccgtcacctg cgccctgacc gaaccgaagt aaacgatgtt ggacgagggg gtcgcattga
       61 aattgttgcc gctgatggtc acgggtgtac ctacgggtcc tctaagcggg gctaccgagc
      121 ttatggtagg ctgggctatg acgatagttg ggacaatagt tgcgatgatc gcaggaagaa
      181 atacgaccag acagaggcaa gcgagccttc gtccgccggg gaataacggt ttgatgccag
      241 ggtagaggtt tggcattagg aattgaattt gggatgaaga taatagaaag aaattgtaac
      301 gcaaaacata tatgcaaaat acatatataa agacgccgcc gggaaaattc agaacttaca
      361 tcgcatgaag cctgtgacct attatgcggc tgcggtaacc gccttcacga tatggggatt
      421 ttttagcctg gtattgcggc cgctggccaa ctatgcagcc ctcgacatcc tgttttaccg
      481 ggtattcatc agcgctgcat tgatgtttcc cgttagcagc ctgatccgtc gcaaagtgtg
      541 ggtggctaac cggcgtctgt tttcggggtt gtcgcatcgt gacaaacggg gtttgctttt
      601 ccaactgttc gggggaggtc tttttctgac gggcaactgg ttctcgtata tctatgtcgt
      661 caacaatgtc agcgtcaagg catcggcctt cgcctatctg gtttgtccag tattgacgac
      721 agtattcgcc tggctgatcc tgaaagagca tctcaccagg ggtcagtggc tggcggtgtt
      781 gctgagcgcc gcgggttgcg gcatactggc tttcaatcat cttgccgatc tgttgtatag
      841 catcgtcgtc gctttctcct atgccctcta tctggtcagt cagcgaaagg attatggcgc
      901 cgaccggttc cttttattaa gcgggcaggt gcttttttca gccttgctgt tgctggccct
      961 ttttccgcgg tttcacggac cggcgccgca ggaatcccga ttttatttcc tgatcgtggt
     1021 tatcgccgtc gcttttacga tctttccact tttcctgaac ctgtatgcac tgaaaggatt
     1081 gcggtcttcc acggtcggga tcctgctgaa catcaacccg ctggtaggtt tcatcctggc
     1141 gatgaccgtt ttcggggaaa aagtggacgt gcagcaggtg gcggcctatt gcgtcatcgt
     1201 ggtggccgtg gccttgttca acttgttcaa cccggtggac cgtgacggca atcgccggcg
     1261 gacacccgat gtatcgattc cggacatgag cggcgttcaa agcggtgggc ggtaactgat
     1321 ttccaatttg ttgaaaaatg ggcatcaaac tggctgcgtc tcccggaaat tctattcatg
     1381 ttcaagaatt actggaagat cgcctggcgc aacctggtca aggaccgcca gttctctctt
     1441 ctcaacctgg ttggtctgtc ggtcggtctg gcctgtgcgt tgctgatcgg actttgggtg
     1501 atggacgagc tgcggatgga aaagtacaat cctaatgacg accggctctt ccaggtgatg
     1561 acgaataaca agacatccaa tggtctgcag acaggcatgt acacgccggg tatcctggca
     1621 aaagcgttaa gggccgagtt tcccgaagtc gaagacgctt cctcggtatt accggcttcc
     1681 tggttcaacg acccgaatac tcccggaggg gtcgtaagct atgatcagaa gaagctgaat
     1741 gcgacaccgc agtacgtcga cagcaactat ttccacctat tccgctgtcc gatcctggag
     1801 ggcgaccgca ggaggctttt cgacggcaag caaggcgtgt tcctttcgtc ttcgctggcg
     1861 caaacgcttt tcgggaccac gcatcacctg atcgggaagg tcatccattt cgaccagtat
     1921 gattttaccg gagacttcga gatacgcggc atctttcaac ccaacccgcc caacgcgtcg
     1981 gaaaaaccgg acctgctttt caacttcgat gtcgcgctgg aaagacgtcc cgggttacag
     2041 cattggtata acgccgaccc gcatacgttc gtacttctca gaccgggcgc cgacccgcgg
     2101 gtcctggatt caaagctttc gcattatttg ctggcgaaac aaggtggcaa gggctgggat
     2161 gcgcagcttt tcctcgctaa gttttccgat cgttatttgt acaaccgtta tgagaacggc
     2221 gtgcagagcg gcggccggat cgtctatgta aggctgttca tcatcatcgc cgtttttatc
     2281 ctcgtcatcg cctgcatcaa ttttatgaac ctgtccaccg cccgggcgac ccatcgggcc
     2341 agggaggtag ggataaaaaa agtcgtaggc gccagtcgca tcgcattgat cctgcaatac
     2401 ctcggcgaat ccgtccttct cacctgttgt gccctggtcg cggccgtggc gatctgcgag
     2461 ctgctgttgc ctgttttcaa cggcatcacg ggcaaacagc tttcctttca tctggcgcca
     2521 aacctactct ttttgctggc cggcgtaacc ctcttgacgg gtctttttgc cggcagctat
     2581 cccgcctttt atctttccgc tttccggccg gtggccgtgc tgaaaggtga cataaagacc
     2641 tcctggaggg aattatgggc acgaaaagga ctggtcgttt ttcagtttac gctgtcgatc
     2701 cttttcatag cgggtgtcct gatcgtgtac cggcagttgt cctatatcca gtcgcgcgac
     2761 ctggggtaca accgggatca tgtgatcgac ttcaacatcc ctattaaatt ggattcgatc
     2821 tatttggtcc atgcggccag ctttgtcaat gagctgaaca atattcccgg tgtcgtcagc
     2881 gccggcagtc actcgcacaa tctgaccggt gaccacggtt cgatcagcgg tgtgtcatgg
     2941 ccgggaaagg atccgggcct ggatatcgaa ttcgccaata tcgaagtcgg ctatcatttt
     3001 ttgcagacga tgggcatcag gctgaaagcg gggcgttatt tctcgcagaa tatggcacat
     3061 gagatcgtta tgaatgaaac ggcgattaag gcgatgggtt tgaaagaccc cctcggcaaa
     3121 gtcgtgaggt tctgggatga gaaacgagag atcatcggaa tagcagccga cttcaatttc
     3181 gaatctttgt atcagccggt gaaacctgct ttcttccgct gttatcctgt cacgaaccag
     3241 gttgtggtac ggatcatgcc gggcgccgaa gcgtcgacga tcgccgcggt cggaaaggct
     3301 tatacgcgct tcaatcccgg tatggacttc gaataccggt atctggatga agactatcaa
     3361 cggttgtatt cctccgaaac gaggattggc gagctttcgc gttattttgc cggtctggcg
     3421 atcctcattt cctgcctggg tcttttcgga ctcgcggcct tcagcgccgc ccgccgccgg
     3481 aaagaactcg ggatccgcaa ggtgatcggc gcatccgtcg ttcagttggt atacctgatg
     3541 tcaaaggaat tcatttggct ggtagcgctg gcgattgcga tcgcattccc gctagcatgg
     3601 gcagggatgc attactggct gaatgaattc gcctatcgca cggatattac ggcggacctg
     3661 ttctggatca cgggtctggc ggtcatcctt attaccctgt tgacgataag ttatcaatcg
     3721 atcagtaccg cgctggcgaa cccgatacga agtctgcgca ccgaataaaa tcttcgatat
     3781 gctgaagaac taccttacta tcgcctggcg ccatatggtg cgctacaagg gctatgccgc
     3841 catcaatatc atcggactgg cactcgggtt ggtctgttgt ctgttcatct ggctatgggt
     3901 gcaggacgag cgtgccgtgg ataattctca cgcagcgggg agtcgcctgt acctggccta
     3961 ttccaccatc atcagcaatg gacgggtcca ggggtcgtac aacacaccgg tgacttacga
     4021 ctatactgcc aacaaaccag tgtttttaac cgaggatgcc ggagccgtcg ttcccggcgt
     4081 gcggcacgtg gtattttaca ggaagggtta tgagcttccc tgggggcatc ctgaatcgtt
     4141 ccaggcgggg gataagatca tccggctcaa tggttcgcgg gcgacggcga attttttcaa
     4201 ggtattcagt ttcccgctga ttgaagggag accggaaacc gcgctatcgg atgccggcag
     4261 catcgcgatc tcgcggaaaa tggctatagc ctttttcggc agcccggaaa aggccatcgg
     4321 caggacgctg aggtatgaga accggcgtaa tttttccgtg acggccgtat tcgaagacat
     4381 cccggcgcaa agttcgctgc aattcgaatt cctgctaccc tggtggtctc aggggaatgg
     4441 gttggcaggg ttggagtact ctaccaataa tttcgatact ttcctggaac tggcggataa
     4501 tgccgacact gcagc
//
LOCUS       DASYSV010000307         8927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1855563, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000307 DASYSV010000000
VERSION     DASYSV010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855563"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /gene="mutL"
                     /locus_tag="VGQ51_09165"
     CDS             complement(<1..1267)
                     /gene="mutL"
                     /locus_tag="VGQ51_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV9036779.1"
                     /translation="MPDIIQLLPEHLANQIAAGEVIQRPASAVKELLENAVDAGATEI
                     RLFVQDGGKSLIQVIDNGKGMSETDARMAFERHATSKIREIDDLFHIKTMGFRGEALA
                     SVAAVAQVELKTRRPEDEAGTRIEIENSRVVKQEPVAMHPGTIISMKNLFFNVPARRN
                     FLKSHAAEMRHVVDEFIRVALSFPSIFFSLSGNGHELLHLEKGSLKQRILQVLGSSFA
                     SKLVSVQENTDYLNIYGFVGKPETAKKTRGDQYFFVNNRYIRSPYLHHAVMNAFTEMI
                     PQECFPLYVLFVDLDPTQVDINVHPTKQEIKFEDEKIVYAFVQAAVKHALAQYSITPT
                     LDFDLDPSIQRLEAVSKPVSEEKKAEVGSSSLYKTFTQKNQAHFIEPSNKSELKHWKD
                     FYEAPSANPTTAAGAASAGGTATPGEETWM"
     gene            1301..4381
                     /locus_tag="VGQ51_09170"
     CDS             1301..4381
                     /locus_tag="VGQ51_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV9036780.1"
                     /translation="MRNYIFLLALMALMFKSQAQSATRTSPNPEAAHWVDSVFKSLTP
                     DEKIAQLMVIRLSGIDPSTRRPIFFDAQVDSLVRKYDVGGICLFQGDPLTQAEHINYY
                     QTVTKTPILFCIDAENGVGMRMDSVIGLPRQMMLGATGDPDLVYRYGRLVGRQCRRVG
                     IQVNYAPVVDINNNPDNPVINDRSFGEDKFNVAEYGVQYMKGLQDEDVMACAKHFPGH
                     GDVSVDSHLDLPVINKDKKALDSLELYPFRALFKAGVGSVMIAHLYIPAIDNTPNRAT
                     SLSYNNVTKLLRKQLRFQGISFTDALEMKGVAKYYPEGDAAVQSLIAGNDMLCLPGDV
                     ALSISKIDSAIKAKRLKWKDIDARVKKVLYAKYEYGLAHWEPVNKAHLTADLNEGVTD
                     MRRQVATEALTLLRDADPSIFPLHAVRDSSMVAAENAGMGRHAGRKKPIAYIGMGLTE
                     DNNFSRRMRTDYNAQVYLFDYNLDSVKAAAALQLLSNRYQTIVIGLHNYNRFPARNFN
                     ISAPAAWLLQQLQQKEKTVTFVFGNPYAIKNSCDAPVLVACYEDDSVTQDVAADLLNG
                     KFTARGHLPVSVCDQWKAGAGITGPARVLSAAAPAALGMDPDQLNKIDDICREGIAKK
                     AFPGCVVLVARDGRIAYEKAFGYTSYDDTEPVYKETIYDMASCTKICATTMSVMKLYD
                     EGKLDLQKTLGDYLPWVRGSDKAPLKIWDILLHQAGLVPDVVFYKEVVDSSKEQNPMP
                     GIFTVRPDSLHDVRVAENLYLRNDWRDTMDLRVVQSKLLTPRRYIYSDNDFIYMGEIV
                     EAISGMTLDQYVKKTFYDPLGMTSSGFRPRDRFPLGRIAPTAMEPIFRHQLIRGDVHD
                     PGAAMFGGVAGHAGLFSDAYDLALLEQMLVDGGTFNGRSYLKQSTIDYFTAYHSDISR
                     RGLGFDKPEKDNAARNAGGKEPYPCLSASPETFGHTGFTGTCVWVDPKYKLVFIFLSN
                     RVNPVENNPLLSRMNIRGRIQETIYQAMGVDMGASTKAPVVEKKKKKHHKKKRS"
     gene            complement(4378..5052)
                     /gene="nth"
                     /locus_tag="VGQ51_09175"
     CDS             complement(4378..5052)
                     /gene="nth"
                     /locus_tag="VGQ51_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV9036781.1"
                     /translation="MTRKERFQFVLDYFSKNNPDAETELLYDNPYQLLVAVILSAQCT
                     DKRVNMTTPAIFERYPDAVTLAKASFEELFPFIKSISYPNNKTKHLIGMANMLVDRFG
                     GAVPLTVEELVQLPGVGRKTANVITSVVDNQPNMAVDTHVFRVAARIGLTINAKTPLA
                     TEKQLLQWIPREQVHVAHHWLILHGRYICVARTPKCSECGLRPACKYYAKNVLKTVVE
                     PVEKKR"
     gene            complement(5089..5331)
                     /locus_tag="VGQ51_09180"
     CDS             complement(5089..5331)
                     /locus_tag="VGQ51_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036782.1"
                     /translation="MTLFTPEDLLRYLYKESSPELTAAIEHALKEDWMLREKLAVLRS
                     STDKLDTITVSPRMEVIQRVMNYARRTSVEESVSHH"
     gene            complement(5504..5821)
                     /locus_tag="VGQ51_09185"
     CDS             complement(5504..5821)
                     /locus_tag="VGQ51_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV9036783.1"
                     /translation="MKLLTHIPGWLKNKYLLTTVGFVVWVLFFDSRDFITSHFRERAQ
                     LVRMENSKLYYEQQIETTKKELQELKTDPALLEKYAREKYLMKRDNEDLFLIRGNNST
                     VKH"
     gene            complement(5882..7162)
                     /gene="eno"
                     /locus_tag="VGQ51_09190"
     CDS             complement(5882..7162)
                     /gene="eno"
                     /locus_tag="VGQ51_09190"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV9036784.1"
                     /translation="MSYIADIHARQILDSRGNPTVEVDVITENNFRGRAAVPSGASTG
                     IHEAVELRDGDKQVFGGKGVLKAVANVNDIIADQLIGFPVNEQASLDARLIEMDGTNN
                     KGKLGANALLAVSMAAAKAAAKESKLPLYRYLGGVNSTVMPIPLMNILNGGVHADNKI
                     DYQEFMIVPMGADSFSDGLRWGADIFHQLKSVLKKKGFSTNVGDEGGFAPDIQSNEEA
                     IETVLQAIESAGYKVGTQIGIALDAASSEMFKDGKYKFYKSTGKEISSDEMVDYWVSW
                     VKKYPIISIEDGMAEEDWDGWKKLTNALGDKVQLVGDDLFVTNTKILQKGIDRGVANS
                     ILIKVNQIGTITETINAVQLAQTHGYTTVMSHRSGETEDTTISDLAVALNCGQIKTGS
                     LSRTDRTAKYNQLLRIEEQLADAAIYPKGRYFKK"
     gene            complement(7181..8092)
                     /gene="gldA"
                     /locus_tag="VGQ51_09195"
     CDS             complement(7181..8092)
                     /gene="gldA"
                     /locus_tag="VGQ51_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HEV9036785.1"
                     /translation="MSIEVKELVKTYGEQKAVDSISFKVDKGEIVGFLGPNGAGKSTT
                     MKIITGYLGADTGEAVVCGIPVSAHPLQAKKKIGYLPEANPLYPDMYVREYLDFIADV
                     HHVPSKKTRIEAVIQLVGLAPESRKQLGQLSKGYKQRVGLAAALLHDPEVLILDEPTS
                     GLDPNQIVDIRDVIRQLGKNKTVLFSSHILQEVEALCDRVIIINKGRLVADSTISRLR
                     TEGDGSDSIRVGFREAPDPAQLQQLDGIKTLTRQSDHEWEIRTDRPDAVKKQLLELAL
                     QNNWNIVSLQSENQSLEAIFRSLTKQD"
     gene            8182..8808
                     /locus_tag="VGQ51_09200"
     CDS             8182..8808
                     /locus_tag="VGQ51_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEV9036786.1"
                     /translation="MTPPPVVTASKEGIANPAPLGLCAFGTTTILLNLHNAGIFEMSS
                     MILAMGIFYGGIAQVIAGIIEAKKNNTFGLTAFTSYGLFWLSLVALIVMPKMGWMTAP
                     SEDSMVAYLIIWGLFTLLLFIGTLKLNRALQMVFATLAILFFLLAAGDSTGNAGLKHF
                     TGYEGIFCGALAIYTGIAGLLNEMYGRTVLPVGAKSLNLNAAPSAAVA"
BASE COUNT         1962 a         2391 c         2346 g         2228 t
ORIGIN      
        1 tcatccaggt ttcttcgccg ggagtcgccg ttccacctgc ggatgcggcg ccggcggccg
       61 tggttgggtt tgcagagggc gcttcataaa aatctttcca atgtttcagc tcgcttttat
      121 tcgatggttc tatgaagtgc gcctggttct tctgcgtaaa ggtcttatag agcgatgagg
      181 atccgacttc cgcctttttt tcttctgaaa ccggtttact caccgcctcc agccgctgga
      241 tcgacgggtc caggtcgaaa tccagcgttg gcgtaatgct gtactgcgcc agcgcatgtt
      301 tcaccgccgc ctgtacgaag gcatatacga tcttttcgtc ttcgaacttg atctcctgtt
      361 tggtaggatg cacgttgata tcgacctgcg taggatcgag atcgacgaag agcacatata
      421 gcggaaaaca ctcctggggg atcatttccg taaaggcgtt catcaccgcg tggtgaagat
      481 aggggctgcg gatgtaacga ttattcacga agaaatactg gtcgccccgc gtctttttgg
      541 ctgtttccgg cttaccgacg aagccgtaga tattgagata gtcggtattc tcctgtacgg
      601 agaccagttt gctggcgaac gaggacccca atacctgtag gatgcgctgc ttcaggctcc
      661 ctttttcgag atgcagcagc tcgtgtccgt tgccggacag ggagaaaaag atggatggga
      721 acgacaacgc cacacggatg aattcatcga cgacgtgacg catttcggcg gcatggctct
      781 tcaaaaaatt cctgcgtgcc ggaacgttga agaacagatt cttcatggaa atgatcgtgc
      841 cggggtgcat cgccacgggc tcctgtttca cgacacggct gttctcgatc tcgatacgcg
      901 taccggcctc atcttccggt cgtctcgttt tcagttccac ctgcgccacg gcggcgacgg
      961 aggccagcgc ttcgccccgg aaccccatcg ttttaatgtg aaagaggtcg tcgatctccc
     1021 tgatcttgga ggtggcatgt cgttcgaatg ccatccgcgc gtccgtctcg ctcattcctt
     1081 tcccgttgtc gatcacctgt atcagcgatt tgcctccgtc ctgcacgaag agccgtatct
     1141 cggtagcccc ggcgtcgacg gcattctcca gcagctcctt cacggcactg gctggtcgct
     1201 ggatcacttc gccggctgcg atctggtttg cgaggtgttc gggtaatagt tgaatgatgt
     1261 ctggcaattc tcccttaatt tgtgtgcaat ttacgcacaa atgagaaact acatctttct
     1321 cctggcattg atggccctaa tgttcaaaag ccaggcacaa tccgctacca ggacctctcc
     1381 caaccccgaa gccgcccatt gggtagacag tgtattcaag agccttacac ccgatgagaa
     1441 gatcgcccag ctgatggtga tccggctttc cggtatcgac ccctccaccc gccgtcccat
     1501 tttttttgat gcacaggtgg attcgctggt ccgtaaatac gatgttggcg gcatctgtct
     1561 tttccagggc gatccgctga cccaggccga acatatcaat tattaccaga ccgtaaccaa
     1621 gactccgata ctcttctgta tcgacgcgga aaatggcgtc ggtatgcgga tggacagtgt
     1681 catcgggctg ccccggcaaa tgatgctggg tgctaccggc gaccccgatc tggtctatcg
     1741 ctatggccgc ctggtaggca ggcaatgccg gcgggtaggc atccaggtga actatgcccc
     1801 ggtagtcgat atcaacaaca acccggataa ccccgtcatt aacgaccggt cattcggcga
     1861 ggacaagttc aacgtagccg aatacggcgt tcagtatatg aaggggctgc aggacgaaga
     1921 cgtcatggcc tgcgccaaac acttcccggg acatggcgac gtatccgtcg attcacatct
     1981 ggatctgccc gtgatcaaca aggataaaaa ggcactggat tcgttggagc tttacccctt
     2041 ccgggcgctc ttcaaagcgg gtgtcgggag cgttatgatc gcgcacctgt atatacctgc
     2101 catcgacaat acccccaatc gggcaacatc gctttcatat aataatgtca cgaaattgct
     2161 gcgaaagcaa ttgcgtttcc agggtatcag cttcaccgac gcgctggaga tgaaaggcgt
     2221 cgcgaaatac tatccggaag gcgatgccgc cgtgcaatcg ctgatcgcgg gtaacgacat
     2281 gctttgtctg cctggcgatg tagctctcag catttcgaag atcgattccg ccataaaggc
     2341 caaacggtta aaatggaagg acatcgatgc ccgcgtcaaa aaggtgctct atgccaaata
     2401 tgaatatggc ctggcgcatt gggagccggt caataaggca catctgacgg ccgatctgaa
     2461 tgaaggggtt accgatatga ggagacaggt cgccacggaa gccctgaccc tattgcgtga
     2521 tgccgatccg tcgatcttcc cgttgcatgc cgtgagggac agctcgatgg ttgctgccga
     2581 aaacgccggg atgggaagac acgccggccg caaaaagccg atcgcttata ttggaatggg
     2641 gctgacggag gacaataatt tcagccgacg gatgcggacg gattataatg cccaggtgta
     2701 tcttttcgat tataacctcg acagtgtcaa ggccgcggcg gcattgcagc tgttgagcaa
     2761 ccgctatcag acgatcgtta tcggactgca taattacaac cggtttcccg cgcggaattt
     2821 caacatcagc gcacctgccg catggcttct gcaacagctg cagcaaaaag agaagaccgt
     2881 caccttcgtc tttggcaatc cctatgccat caagaacagc tgcgatgccc cggtgttggt
     2941 ggcctgttat gaggacgact ccgtcacgca ggatgtggct gccgaccttc ttaacggcaa
     3001 attcacggcc cgcggtcatc ttccggtgtc ggtctgcgac caatggaagg cgggcgccgg
     3061 gatcacggga cccgcacgcg tgttgtctgc cgccgctccg gccgcattgg ggatggatcc
     3121 cgaccagttg aacaagatcg acgacatctg tcgtgagggc atcgcaaaga aggcgttccc
     3181 gggatgcgtc gtgctggtag ccagggatgg aaggatcgcg tatgaaaaag cattcgggta
     3241 cacgtcgtat gacgatactg agccggtata taaagaaacc atctacgaca tggcctcctg
     3301 taccaagatc tgcgccacca cgatgtcggt gatgaagctc tatgatgagg gtaagctgga
     3361 tctgcagaag accctggggg attatctgcc ctgggtgcgc gggtccgata aggctcccct
     3421 caagatctgg gatatcctgt tgcatcaggc ggggcttgtg cccgatgtag tgttctataa
     3481 ggaagtggtg gacagtagca aagagcagaa cccgatgccc ggtatcttta cggtgcggcc
     3541 cgactcgctc catgacgtca gggtggcgga aaacctctat ctgcgcaatg actggcgcga
     3601 tacgatggac ctcagggtcg tgcagagtaa actgctgacc ccccgcagat atatctatag
     3661 tgacaatgat ttcatctata tgggtgagat cgtggaagcg attagcggca tgactctcga
     3721 ccaatacgtc aaaaaaacct tctacgatcc gttgggcatg acctcttctg gtttcagacc
     3781 ccgcgatcgt ttcccgttgg ggcgcatcgc ccccaccgct atggagccca tcttccgtca
     3841 tcagctgata cgcggtgatg tccatgaccc cggcgcagcg atgttcggtg gcgttgccgg
     3901 tcatgccggc ctgttcagcg atgcgtacga tctcgccctt ctcgagcaga tgctggtgga
     3961 tggcggaacc tttaatggcc gcagctatct caaacagtcg accatcgatt atttcacggc
     4021 gtatcacagc gatatcagcc gccgtggtct cggctttgac aagccggaaa aggacaacgc
     4081 cgcccgcaat gccggcggca aggaacccta tccctgtctc tcggcttcgc ctgaaacgtt
     4141 cggccatacc ggcttcaccg gcacctgtgt ttgggtggac ccgaaataca agctcgtgtt
     4201 catcttcctt tccaatcggg tgaatcctgt cgagaacaat cctctccttt cgcggatgaa
     4261 tatccgcggg cggatacagg agacgatcta tcaggcgatg ggtgtggata tgggcgcctc
     4321 gacaaaggcg ccggtggtgg agaagaaaaa gaaaaagcac cataagaaaa aacgctccta
     4381 gcgctttttc tccacgggct caaccaccgt cttcaacaca tttttcgcat agtatttaca
     4441 tgccggccgc agtccgcatt cggaacattt cggcgtccgt gccacgcaga tatacctgcc
     4501 atgcaggatc agccagtgat gtgcgacatg cacctgctcg cgcgggatcc attgcaaaag
     4561 ctgtttttcc gttgccagtg gcgtcttcgc atttatggtg aggccaatac gggcagcgac
     4621 ccggaaaaca tgcgtatcaa cggccatatt cggctgatta tcgactacgg aagtgatgac
     4681 attggccgtc ttgcggccga caccgggcaa ctgcaccagt tcttcgacgg tcaggggaac
     4741 agctccgcca aaccggtcga ccagcatatt cgccataccg atgagatgct tggtcttgtt
     4801 gttagggtat gaaatgcttt tgatgaaggg gaagagttcc tcaaaagaag ctttggccag
     4861 cgtcaccgcg tcagggtagc gctcgaagat ggccggagtg gtcatattga cccttttgtc
     4921 cgtgcattgt gccgaaagga ttaccgctac cagtaattgg tagggattgt catacaaaag
     4981 ctcggtctcg gcatccggat tgttcttgct gaagtagtcc agtacgaact gaaagcgctc
     5041 ctttctagtc attagattat gaccgcgaag ttacgacaga agaagtactc agtgatgaga
     5101 tacagattcc tcgacggaag ttctcctggc ataattcatc actcgctgaa taacctccat
     5161 gcgtggggat acagtgatcg tatctaactt gtcggtagaa gatcgcagaa cggctaattt
     5221 ttcgcgcagc atccagtctt ctttaagcgc atgctcgatc gccgccgtta attccgggga
     5281 agactcttta tacaaatacc gcaaaagatc ctccggtgta aagagtgtca taggcaaaac
     5341 catttaatgt cctaatatgt tccgaaagca gtaaaagtga aacgtccgta tataaaacga
     5401 cattcggatg gccttattgt ccggtaccgt aatatttgtt ccctgcggac gccgcacatg
     5461 gtttagctat aaagggagcg ggagaaatgc aaaatgctga tcgtcaatgt ttaacagtcg
     5521 aattattgcc ccggatcagg aagagatcct cattgtcccg tttcataagg tacttttccc
     5581 gggcatattt ttccagtaag gccgggtccg tcttcagctc ctgtaattcc ttttttgtcg
     5641 tctcgatctg ctgttcgtaa tacagtttgc tgttttccat cctcaccaac tgtgcccttt
     5701 cccggaaatg gctggtgatg aaatcccgcg agtcaaagaa caatacccat actacaaaac
     5761 caaccgtggt aaggaggtat ttgttcttca accagcctgg gatatgcgtt aaaagcttca
     5821 taaaacacgg tggcggccca ttccttcttg tctggcgttc ggccgccacc gaaattaatg
     5881 attatttttt gaaatatctg cctttcggat aaatggcggc atcggccagt tgttcctcga
     5941 tacggagcag ctggttatat ttggccgtcc ggtccgtacg cgagaggctg ccggtcttga
     6001 tctggccgca gttaagggcc actgccagat cggatatggt agtatcttcg gtctccccgc
     6061 tgcggtgtga catgaccgtt gtataaccgt gagtctgagc aagttgcaca gcgtttatgg
     6121 tttccgttat cgtccctatc tggttcacct tgatcaggat gctattagcg accccccggt
     6181 cgataccttt ctgcaggatt tttgtattcg tgacgaaaag gtcgtctcct accagctgta
     6241 ccttgtcgcc gagggcattc gtcagctttt tccaaccatc ccagtcttct tcggccatcc
     6301 cgtcctcgat ggagatgatc ggatatttct ttacccagga aacccaataa tctaccatct
     6361 catcgctgga tatctccttc ccggtactct tatagaattt gtatttgccg tctttgaaca
     6421 tttcgctgct agcggcatcc agggcgatac cgatctgggt tccaaccttg tatccggccg
     6481 attcgatggc ttgcaggact gtttcgatgg cttcctcatt gctttggata tccggcgcga
     6541 aaccgccttc atcaccgaca ttggtgctaa atcctttctt tttcagcacc gacttcagct
     6601 ggtggaagat atccgcgccc cagcgcagac catcgctgaa ggaatctgcg cccatcggca
     6661 cgatcatgaa ttcctggtag tcgatcttat tgtcggcatg caccccgcca ttcaggatat
     6721 tcatcagcgg tatcggcata acggtactgt tgacgccgcc gaggtaacgg tataatggaa
     6781 gcttgctctc ctttgcggca gccttcgctg ctgccatgct gacggccaat aatgcattgg
     6841 cgccgagctt gcctttatta ttggtgccgt ccatttcgat cagccgggca tccaggctgg
     6901 cttgttcgtt aacgggaaaa ccgatcagct ggtctgcgat gatatcgttc acgttggcta
     6961 ctgccttcag cactccctta ccgccaaata cctgtttatc tccgtcgcgc aattcaacgg
     7021 cctcatggat gcccgtcgaa gcgccgctgg gcacggccgc gcgaccgcga aaattatttt
     7081 ccgtaataac atcgacttcc accgtaggat ttccacggct atccaggatc tgcctggcat
     7141 gaatgtcagc aatgtagctc atgataatgt gttgataatt ttaatcttgt ttcgtcaggc
     7201 tccggaaaat ggcttccagg ctttgatttt cgctttgcaa agaaacaatg ttccaattgt
     7261 tctgcaaagc cagttccagc aattgttttt tgacggcatc gggcctatcc gtccggatct
     7321 cccattcgtg gtcggattgc ctggtgaggg tttttattcc gtcgagctgt tggagttgag
     7381 cagggtccgg ggcctcccgg aacccgacgc gtatgctgtc cgatccgtcg ccttcggtac
     7441 ggagcctgct gattgtgctg tccgctacca atcggccttt attgatgatg atgacgcggt
     7501 cgcagagggc ttctacctcc tggagaatat gcgaagaaaa aaggaccgtc ttatttttgc
     7561 ccagctgccg gatcacgtcg cgaatgtcga cgatctgatt cggatcgaga ccgctggtgg
     7621 gttcgtccag gatgagcact tcaggatcgt gcaatagcgc ggctgcgagt cccacgcgtt
     7681 gtttgtatcc cttggacagc tgtcccagct gtttgcggct ttccggcgcc agacccacta
     7741 actggatcac tgcctcgatc cgggtctttt tcgaaggaac atgatgaaca tcggcgatga
     7801 aatccaggta ctccctcaca tacatatcgg ggtacaacgg gttggcttca ggaagataac
     7861 cgatcttctt tttcgcctgc agagggtggg cgctgacagg tataccgcaa acgactgcct
     7921 cgccggtatc ggctcccagg tagccggtaa tgatcttcat ggtagtggat ttacctgccc
     7981 cgttgggtcc gaggaaaccg acgatctctc ctttatctac tttgaaggaa atggaatcta
     8041 cggccttttg ctcaccataa gtctttacga gctctttaac ttcaatcgac atgcagcgga
     8101 tttacaccgt aaagttaggc taatctgata aaatgcttta aacttgtagc attaagccac
     8161 tttaatcttc aactaacgat tatgactccc cctcctgtcg tcacagccag caaggaaggc
     8221 attgccaacc ctgcgcccct gggcctttgc gccttcggta ctacaacaat attgttgaac
     8281 ctgcacaatg ccggcatctt cgagatgagc tccatgatcc tggcgatggg catcttctat
     8341 ggcggaattg cccaggtgat cgcgggtatc atcgaggcga aaaagaacaa tacgttcggt
     8401 ctgaccgcct tcacttctta cggtttgttc tggctgtcgc tggtcgccct catcgtcatg
     8461 ccgaagatgg gttggatgac ggccccgtcg gaagacagca tggtcgccta cctgatcatc
     8521 tggggattgt tcacgctatt acttttcatc ggaaccttga aactgaaccg cgcattacag
     8581 atggtcttcg ctacgctggc cattttgttc ttcctgctgg ccgcgggtga ttcgacgggg
     8641 aatgccggtc tgaaacattt caccgggtat gaaggcatct tttgcggggc attggctatc
     8701 tatacgggta tcgccgggct gttgaatgag atgtatggcc ggaccgtcct gcccgtagga
     8761 gcgaagagcc tgaaccttaa cgccgccccg agtgcggctg tcgcataaac acattcagat
     8821 gagttaccct taccagataa aatcaccgga agaatatcgt tccgcttatg agaccagtgt
     8881 aaaggatccc gaaggatttt gggccggcat cgcatcgcag ttcgcct
//
LOCUS       DASYSV010000308         4802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_315457, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000308 DASYSV010000000
VERSION     DASYSV010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315457"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VGQ51_09205"
     CDS             <1..1039
                     /locus_tag="VGQ51_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9036787.1"
                     /translation="IRNKMNARRDLASPLTETLGIVILTIILYYGGMLIFHTKGNGFS
                     GGDLITYIAVFAMLINPAKTLSTSIFNVQRGAAAMERVEDLLHAPVTIHDDPQAKKLE
                     AFRDRIEFRDISFSYEDAGILENIDLVIEKGKTIALVGSSGAGKSTLADLIPRFHDVT
                     GGRLLIDGTNIKDYSLRSVREQMSIVTQEPILFNDTIAANIALGKPDATMDEIIRAAT
                     IANAHSFITAKEEGYQANIGDRGSKLSGGERQRLTIARALLKNPPILILDEATSSLDT
                     ESERLVQDAINKMMEHRTSIVIAHRLSTIRHADEIIVLQKGRIVERGTHDQLMQLQGF
                     YRKLVEMQEVK"
     gene            1048..1575
                     /locus_tag="VGQ51_09210"
     CDS             1048..1575
                     /locus_tag="VGQ51_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036788.1"
                     /translation="MRTPLTSPPPTFKWVYITFGAAICFAIIFEIAVRSSPTHPAFEI
                     VCVLGAMLSFAFTIGQTTNVVTYRYLRKPLEFDEHNLYIMRKDGETAIPFTDILEIRY
                     AFYNKNSFRFNANYRIRYRLDDRGQEILITVFRDNRPTFDQFTKLLQTVNPLLKITNW
                     SITGEDLLWRFRNRK"
     gene            complement(1628..4237)
                     /locus_tag="VGQ51_09215"
     CDS             complement(1628..4237)
                     /locus_tag="VGQ51_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEV9036789.1"
                     /translation="MRLVLPFLLAASVSAIAQPTPVTTANYQAAARFSPRKMEKMVFT
                     LTVDPHWLKKSNRFWYMYETTEGKKWYIVDPVKGEKREMFDRDKLAAGISLVVKDPFD
                     AKHLGLDSLKFIKDENWIQFEVKSSQDADKKDSAEGAMGNGGGGNRRRGGGGEAGAGG
                     AGARQKKVYYFEYNLVDGTLNELTGYKKPKHKPNWANISPDSNTIVFGRHFNLYWMDR
                     ANYEKALKNEDDSTIVEHALTSDGMENFSYHGTGAVGGGGETNVDKEKNKDKRKPAQL
                     FWSPDSKHFAMSRSDQRKVKDLWVINSLAEPRPTLETYKYMMPGEKEAPVEQLVVFDM
                     TAHTHQAVGVAEFKDQDLAIWSAPPKGRHRDDEYRVQYWLGTNTKFYITRTSRDLKRI
                     DVCAVAVGDTVARPLIQERLNTYVELRRLGLVNEGRELIEWSERDGWAHFYLYDGSGK
                     LENRITSGPFHCEDIIGIDDKKRILYFTANGKESGEDPYFLHVYKVNLDGSGLALLDP
                     GDFDHLGEMDDDEEFFVDNFSRVNTVPKSVLYSSSGRKVMDLETADLSSLFEAGYRFP
                     QPFKVKADDGITDLYGVMYKPFNFDSTKKYPLMEYVYPGPQTEAVSKAFTRGLDRTDR
                     LAQLGMIVITVGNRGGHPSRSKWYHNYGYGNLRDYGLADKKAAAEQLADRYSFIDINR
                     VGIYGHSGGGFMTSAALLVYPDFFKVGVSSSGNHDNAIYNRWWSEKHHGVKEMVSDKG
                     DTTFLYSIEKNPEIASHLKGHLLLSTGDIDNNVHPGNTIRLANALIKADKRFDLVLLP
                     GQRHGYGDMTEYFWWRMADYFSYWLIGDKTVRPISIDEMDNEVPQTGAKAGSNREPSG
                     WGDDDDDDGDWDY"
     gene            complement(4436..>4802)
                     /locus_tag="VGQ51_09220"
     CDS             complement(4436..>4802)
                     /locus_tag="VGQ51_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEV9036790.1"
                     /translation="RYSDAELGEFRELINRKLDAAKKELAYLQGLITRKDEMGGDNDD
                     ARYMTMEDGSMSMEREQLSQMASRQITFIDHLEKALMRIENKTYGICRVTGKLIDKAR
                     LRAVPHATLSIEAKQMMNK"
BASE COUNT         1085 a         1517 c         1058 g         1142 t
ORIGIN      
        1 tatccgcaac aagatgaacg cccgccgcga cttagcctcc ccacttaccg agacgctggg
       61 catcgtcatc ctgaccatca ttctttatta tggcgggatg cttatcttcc atacgaaagg
      121 caatggtttt tccggaggtg atctgatcac ctatatcgcc gttttcgcga tgctgatcaa
      181 ccccgccaag accctgtcga cgtctatatt caacgtacaa cgcggcgcag cggcgatgga
      241 aagggttgaa gaccttctcc atgcgccggt aaccatccac gacgacccgc aggctaaaaa
      301 actcgaggcc ttccgcgaca ggatcgaatt ccgcgacatc agcttctcct atgaagacgc
      361 cggcatcctc gagaatatcg atctggtcat cgaaaaagga aagaccatcg cgctcgtcgg
      421 cagctccggg gcaggcaaat ccaccttagc cgatctgata ccccgttttc atgacgtcac
      481 cggcggcagg ttgctcatcg acggtactaa catcaaggac tattcgctcc gctcggtccg
      541 ggaacaaatg agcatcgtga cacaggagcc catcctcttc aacgatacca tcgccgccaa
      601 tatcgcgctc ggcaaacccg acgccaccat ggacgagatc atccgggcag ccaccatcgc
      661 caatgcgcat agttttatca ccgcaaaaga agaaggttac caggccaaca tcggtgaccg
      721 cggcagcaag ctcagtggcg gcgaacgtca acgcctgacc atcgcacgcg ccctcctgaa
      781 aaatcccccc atcctcatcc tcgacgaagc cacctcctcg ctcgacaccg aaagcgaacg
      841 cctcgtacag gacgctatca ataagatgat ggaacaccgc accagcatcg tcatcgccca
      901 ccggctcagc accatccgcc atgccgacga gatcatcgtc ctccaaaaag gccgtatcgt
      961 cgagcgtggt acccacgatc agctgatgca gctacagggc ttttaccgaa agctggtgga
     1021 aatgcaggaa gtgaagtaaa ttcacctatg cgaacacccc taacctctcc gccgccaacc
     1081 tttaaatggg tatatatcac ttttggcgcc gcgatctgct ttgcaataat cttcgaaata
     1141 gcagtccggt catcccctac gcaccctgcc ttcgagatag tatgcgtcct cggcgcaatg
     1201 ctatcattcg catttacgat cgggcaaacg accaacgtcg tcacataccg gtatctccgc
     1261 aaaccattgg aattcgacga acacaatctt tatataatga gaaaggatgg cgaaactgcc
     1321 atccccttta cagatatcct cgaaatacgc tacgcattct ataacaagaa ctcattccgc
     1381 ttcaacgcca attatcgcat ccgctaccgt ctcgatgacc gcggtcagga gatcctcatc
     1441 accgtcttca gggacaatcg gccgacattc gatcagttca caaaattgct gcagacagtt
     1501 aacccgctct taaagatcac caattggtcc atcaccggcg aagacctcct ctggcggttc
     1561 cggaaccgca aatgatcgcc cccgacgccc accgaagcct ccgcgcgtcc atccggggtg
     1621 cctgccatta ataatcccaa tccccatcat cgtcatcatc atccccccag ccggaaggtt
     1681 cccggttact ccccgccttc gcccccgtct gtggaacctc attgtccatc tcatcgatag
     1741 atatcggcct caccgtttta tccccgataa gccaatacga aaaatagtcg gccatacgcc
     1801 accagaaata ttccgtcatg tccccatagc catgccgctg cccgggcaac agcaccaggt
     1861 caaaccgttt gtcagccttg atcagcgcat tcgccagccg gatcgtattc cccggatgca
     1921 cgttattgtc gatgtcgccc gtcgacagca acagatgccc cttcaaatga ctcgcgatct
     1981 ccggattctt ttctattgaa tacaggaatg tcgtatcccc cttgtcgctt accatctcct
     2041 tcaccccatg atgcttctca ctccaccagc ggttgtagat cgcattgtca tgattgcccg
     2101 acgacgacac gcccaccttg aagaaatccg gataaaccag taacgcggcc gaagtcatga
     2161 agcctccccc actatgtcca tagatcccaa ccctgttgat atcgataaag gaataacggt
     2221 ccgccaactg ctcggccgca gcctttttat cggccagccc atagtcccgc agattgccat
     2281 atccatagtt gtgataccat ttgctgcgtg aaggatgacc gccgcggtta ccgacagtga
     2341 tcacgatcat ccccagctgc gcgagccgat ccgtcctgtc taacccgcgg gtaaaggcct
     2401 tgctcaccgc ctccgtttgc ggaccggggt agacatattc cattaaagga tactttttgg
     2461 tgctatcgaa attgaagggc ttatacataa cgccatatag atccgtgatc ccatcatccg
     2521 ccttgacctt gaatggctgc gggaaccggt accccgcctc gaacagcgag ctcaggtccg
     2581 ccgtctccag atccattacc ttcctgccgc tgctgctgta aagcaccgac tttggcaccg
     2641 tgttcaccct cgaaaaatta tcgacaaaaa actcctcgtc gtcatccatc tccccgagat
     2701 gatcgaaatc ccccgggtcc agcaacgcca atccgcttcc atccaggttc accttgtaga
     2761 catgcagaaa ataagggtct tcacccgatt ctttcccgtt cgccgtgaag taaagtatcc
     2821 gtttcttatc gtcgatgccg atgatatcct cgcaatggaa aggtccgctg gtaatgcggt
     2881 tctccaattt cccgctgccg tcatagagat agaaatgcgc ccatccgtcg cgctcgctcc
     2941 attcgatcaa ctcccgtcct tcgttcacga gcccgagccg gcgcagctcg acataggtat
     3001 tcagacgctc ctgtatcagc ggcctggcca ccgtgtcacc taccgccacc gcacatacgt
     3061 cgatgcgttt cagatcgcgg ctggtacggg tgatatagaa tttcgtattg gtgccgagcc
     3121 agtattgtac ccggtattca tcgtcacgat gccgtccctt cggcggagcc gaccaaatgg
     3181 ccaggtcctg gtccttgaat tccgcgacgc cgaccgcctg gtgggtatgc gccgtcatat
     3241 cgaagacgac cagctgttcc accggcgcct ctttttctcc cggcatcata tatttatacg
     3301 tttccagcgt agggcgcggc tcagccagcg agttgatgac ccagaggtct tttaccttcc
     3361 gctgatccga acgcgacatc gcgaaatgtt tcgaatcggg actccagaac agctgggcgg
     3421 gtttccgttt gtccttgttc ttttccttgt cgacattcgt ctctcccccg ccacccaccg
     3481 cgcctgtgcc gtgatagctg aaattctcca tgccgtcact cgtcagcgcg tgctcgacga
     3541 tcgtgctgtc atcttcattc ttcagcgcct tttcataatt ggccctgtcc atccaataga
     3601 ggttgaaatg cctgccaaag actatcgtat tgctgtcggg ggagatattg gcccagttgg
     3661 gtttgtgttt aggcttctta tagccggtaa gctcgttgag cgttccgtcg accaggttgt
     3721 attcgaaata atagaccttc ttctgccttg cgccggcgcc acctgcaccc gcttcaccac
     3781 cgcctccgcg acgacgatta ccccctccgc cattacccat agctccttca gcagaatctt
     3841 ttttatcagc atcctgcgaa ctcttcacct cgaactgtat ccaattctca tccttgatga
     3901 acttgagact gtccagtccc aggtgcttcg cgtcgaaagg gtccttcacc accagtgaaa
     3961 tcccggcagc aagtttgtcg cggtcgaaca tttctctctt ctcgcccttc accgggtcga
     4021 cgatatacca ttttttaccc tccgtcgtct catacatgta ccaaaaacga ttggacttct
     4081 tcaaccaatg ggggtcgaca gtgagcgtga agaccatttt ttccattttc cgcggtgaga
     4141 aacgagcggc tgcctggtaa ttggcggtgg ttacgggcgt tggctgggca atggcgctga
     4201 ccgacgccgc cagcaggaag ggtagtacaa gtcgcataag caggtatttt gtataaaatg
     4261 aatacgctac cagccgcctg atctcagtgc cgactaatat cctaccagcc gcctgatctc
     4321 agcgccaata aatattcccc cagccggccg atctcagtgc cgactaatat cccaccagcc
     4381 gaccgatctc agcgccaggg aaagtaaaag actattatac gggtggcagc gtttcctact
     4441 tattcatcat ctgcttagcc tcgatactca gcgtagcatg tggaacggct ctcaatcttg
     4501 ccttatcgat cagtttaccg gtgacccggc agataccata tgtcttattc tctatcctca
     4561 tcagggcttt ctccaaatgg tcgataaaag tgatctgacg gctggccatc tggctcaatt
     4621 gttctctttc catgctcatg cttccatctt ccatggtcat gtagcgggcg tcatcgttat
     4681 cgccccccat ttcatccttg cgcgtgatca atccctgcag atacgccagt tctttcttag
     4741 ctgcatccaa tttgcggttg atcaattccc tgaattctcc aagttctgca tcgctatatc
     4801 tc
//
LOCUS       DASYSV010000309         9424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_442643, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000309 DASYSV010000000
VERSION     DASYSV010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442643"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VGQ51_09225"
     CDS             <1..375
                     /locus_tag="VGQ51_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEV9036791.1"
                     /translation="PRDTTTLGGTWYLQAVLPSDTATGRAPELDLDLAKSHFAGNTGC
                     NSMRGTFYFSTKDSSLSFSARIVQTRMSCAGYNETGFMKSLQATGRFRLYNGTLTLLA
                     DDKTELSQWARQPGAGAKALKT"
     gene            449..724
                     /locus_tag="VGQ51_09230"
     CDS             449..724
                     /locus_tag="VGQ51_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036792.1"
                     /translation="MANALVDLTRELLNSGIFNHLPDNEIARLHWMILQGQREEQLPL
                     KPLFSYWYRGDYYSSNASPRLLQQCNEYLQRMGQPLIDVYGEEFYEA"
     gene            complement(735..1100)
                     /locus_tag="VGQ51_09235"
     CDS             complement(735..1100)
                     /locus_tag="VGQ51_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036793.1"
                     /translation="MSKELYFDIHGEDSGGSLYRVTEPNGEIAFLYNHSVSDPATGEV
                     EVFDTWYISFEGFWKRLTEDPEWYYRHPLFVHPDQRDFVRQRLKDVNWGIYPDKKWQE
                     SHQRQWKKVLTDPTTYYRR"
     gene            1150..1305
                     /locus_tag="VGQ51_09240"
     CDS             1150..1305
                     /locus_tag="VGQ51_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036794.1"
                     /translation="MALIPNNKNKKDKKQPAAPKSNIPGFPSGKGKTSKGAVKNTRFT
                     GGAQRGS"
     gene            complement(1290..6566)
                     /locus_tag="VGQ51_09245"
     CDS             complement(1290..6566)
                     /locus_tag="VGQ51_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEV9036795.1"
                     /translation="MEPNRILFKDSLSSDDTIAFVLPLFKKVLALHEAGLVGPFEKQE
                     AIFVTDGVADIDESMAHAPCNAPYRVQALFPHAGPFETAGQSEQAFYLAGYRCFEHDL
                     GHHDPQTDIFCLGLVLASIAMGLDLYDPNDLDCFIKLRQHPTQDVPRIHPTLGRLITE
                     MTEPDRSRRSQDLYDAIARLEHYRDYDPEKQADLSALPGWLDRDLKERDAYILNRLRN
                     RLFDTSRRNRLLYFKPNARFVNLTVGSIPLVLHYRSIRPDLLFTWSPELGRLIVGCDE
                     VVLNKYLRFDDHPYLPASLDKVRVEAQRDMQEYGFSQLKLVIAFLEWRNLKDDPRESI
                     RSPLLLVPVTLKKNRRPREDHYILKVSDSIAEVNPVLAGELRELHGIRLPDFVDLEEM
                     SPEEFYRVVKSQIEEAGQGIVLRYVGKPRIALVQQEAAQIVGKYRNKAARSRQTDPQD
                     PAPPPESGTSEEDTTPAEQYPAPEIAAPEIAGPEIAEPSATADVDSNPYDWDFDVCNM
                     VLGNFHYKKMSLVRDYNLVIDQRMQHPTFDSLFGDLPRLFPEHPFDGNRPDDWFHVVS
                     ADPTQTRAILQSRAGYSYIIQGPPGTGKSQTITNLIADFTARGKSILFVCEKRAALDV
                     VYHRLKQVGLEELCCYIHDSQGDKREFIKNLKTTYEDFIDRRMDLPGLKERRESLLRR
                     MSRHLDLIREFHETALVRDKDTGITVRSLLESIIALREKLPVLDPGEEELLPSYSEWK
                     AAGPILQMLDAVMEETGSGSVFSEHPFSRIQEAIVTMSESPYRTLENALQEALLSIAE
                     VETLLIPCGLEPRQLKEHDRLKSLVQFAVLLYPLARSGTMALADPDSEESRQLEKEFL
                     VYRRQQEALRMASEKNSHWKHKLNEQDLDSAIILTTRLEGTFFGRFNGAYRKLKRRLR
                     ESYDLSAHAVTPSWSSLLHLLKTEYEAAAVLEQTRQQLRERFALGPNIDTTRLSIEML
                     QNRNGHPELAWLLHHPRGEIVVTALAAVHQPLLRLESAVNRCLQDPPAGPLSMISDQL
                     LNIRLNADALPAWLPALRGFAGLPDSVKTTLRQLPLRIEQVEAAVARKTLQHLYQTHR
                     EYAGIDGIALENAVQRLGEDYSTLQEVNAELIRAFVRQRFLQQVELSNRAPGQLTEEQ
                     KKFKKAYAEGRKILENEFGKSMRFKSIRELSEKESGLVLKALKPVWLMSPYSVSDSLP
                     LDEDHFDVVIFDEASQITLEEGVPALYRSQQTIIVGDEKQMPPTDFFGTRADDPEDLD
                     RNIETDDEGERMTIDADSLLAQGARKLESTLLSWHYRSHSETLISFSNHAFYEGGLLT
                     IPDKAIHHRAKEPIAVAGPQDAGFGVDSLFDRSISFHLQTGSVYEKRTNPGEAAYIAH
                     LIRELLRRRVPESIGIVAFSLEQGHAIEDALENLAREDVDFARLLEEAYNRTENDQFT
                     GLIVKNLENIQGDERDIIIISVCYGADRRGRMVMNFGPVNKKGGEKRLNVLFSRARKH
                     MAVISSMRFEQITNEYNEGANYLRRFLQYAEHVSGGRMTAARSILDGLIDHRDPDGHA
                     VKATLVRQQLKEQLLAHGYEVEGPIGQSDFKCSLAVKKKGEDEEYALALLIDDEDHYR
                     NDNRIEQYYLRPAALRSFGWKVLPVYAKDWLHQPQKVMEQILKILGHQSTEKPRGMIG
                     GMGPAGAGGSTGVVGAAGLNPDLSFHRLVRLTDDVFWEAATDGNRLILNWGRNGARGQ
                     TNQRSFDDEGSARQELERLEKEQRKNGYQDPR"
     gene            6615..8972
                     /locus_tag="VGQ51_09250"
     CDS             6615..8972
                     /locus_tag="VGQ51_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV9036796.1"
                     /translation="MRDYISELNEQQREAVLHKDGPIMIVAGAGSGKTKVLTTRIAHL
                     LAQGVSAFNILALTFTNKAAKEMKERVEKILGNNEARSLYIGTFHSVFARILRGEAHL
                     LDGYDRNFTIYDTDDAKSVLKTVINELNLDDKHYKPNMVYNRISAAKNSLAGPEEYAN
                     DYGIQQEDMRANRPAIAQIYKAYVDRCKKNGAMDFDDLLMKMYELLKKFPESLHKFQH
                     KFKYILIDEYQDTNPAQYEIIKLLGAAHENVCVVGDDAQSIYSFRGATIENILQFQKD
                     YDDVKVVKLEQNYRSTQSILNVANEVIRNNKGQIPKNLWTENAPGEKIRLVRLMTDND
                     EGRFVADTIQEQRLRNHYNNRDFAILYRTNAQSRAFEESLRRMGIPYTMYGGISFYQR
                     KEIKDFIGYLRIIVNPRDEEALKRIINYPARGIGKTSIDRAVLAANENNISLWEVFER
                     ASEFGLRAGTLEAVDNFVVMIRSFKSMLATKNAYEVAVHVGKQTNLVKELFNDKSTEG
                     VARYENIQELLNSIKEFTERPDDDGVLESETKGLGDYLQQITLLTDADEKDPNADTVK
                     MMSIHAAKGLEFSCVFAAGLEEMLFPNAMAINTREELEEERRLFYVVITRAKQRLWIT
                     YANTRYRFGNLVQNEPSRFIEEIPSEYMDRSFAGGGVRNQGFGGGPSGSAYDRMHGGG
                     FGSSAQQAEKRYGPPPAKKEDARPSYVPPKPYMKTIEHTPSPDFVASDTSGLQVGQKV
                     EHQKFGFGEVIGMEGSAHNPIATVKFQYNGEKKIMLNFAKLRIVE"
     gene            9168..>9424
                     /locus_tag="VGQ51_09255"
     CDS             9168..>9424
                     /locus_tag="VGQ51_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036797.1"
                     /translation="MTDLYKVQPQGLYFVTLTVVGGIDVFTRHEYCDLLVDNLNYSID
                     NKRLRVYEYSILPSQLYIIADVEEGKSNLPKVLRDFKSYSA"
BASE COUNT         2180 a         2557 c         2555 g         2132 t
ORIGIN      
        1 cccagggaca cgactactct tggcgggaca tggtatcttc aggccgtgct gccatcggat
       61 acggctaccg gccgggctcc agagctggac ctggacctcg ccaaatcaca ttttgccggc
      121 aacaccggtt gcaacagcat gcgcgggacc ttctacttca gcacaaagga tagcagtctg
      181 tccttcagtg ccaggatcgt ccagacgagg atgtcctgcg cgggatataa cgagaccggg
      241 ttcatgaaaa gcctgcaggc aacgggcagg ttccggctct ataacgggac gctgacattg
      301 ctggcggatg acaaaaccga gctttcccaa tgggcgcgcc aacccggtgc gggcgccaag
      361 gcgttgaaga catgatcaca gtaggataac acaaacttta caatgtggta acataattgc
      421 aggaaattac aaacaaacag cagctaatat ggcaaacgct ttggtggatt tgacccggga
      481 actgctcaac agcgggatct tcaatcatct tccggacaac gagatcgcac gtctgcactg
      541 gatgatcctt cagggacaac gcgaagagca gctccccctg aagcccctgt tcagctattg
      601 gtaccgggga gattattatt cctccaacgc ttcaccccga ctcctgcagc aatgcaacga
      661 gtatttgcag cgcatgggcc agccgctcat cgatgtatac ggggaggagt tctacgaggc
      721 ctgacgagac gatatcatcg tctgtagtag gtagtgggat cggtcaatac cttcttccac
      781 tgccgctgat gactctcctg ccacttttta tccggataga tcccccaatt cacgtctttc
      841 agccgctgcc gcacgaaatc gcgttgatcc ggatggacga acaagggatg acgataatac
      901 cattcgggat cttccgtcag tcgcttccaa aacccttcga agctgatata ccacgtatcg
      961 aaaacctcca cctcccccgt cgccgggtcg ctgacgctgt ggttgtataa gaaagcaatt
     1021 tcgccgttgg gctccgtcac cctgtaaaga ctgcccccgc tgtcttcacc atgaatgtcg
     1081 aaatagagtt ctttgctcat agattgacgg gaaaatagta ttttcgatca acggaaaaaa
     1141 taatattcta tggcactcat accaaataac aagaataaga aggacaaaaa acagccggct
     1201 gctcccaaaa gcaatatccc cggattcccc tccgggaaag gtaagacgag caaaggcgct
     1261 gtgaagaata cccgttttac gggcggcgct cagcgcggat cctgatatcc gttttttctc
     1321 tgctcctttt ccagacgctc cagctcctgg cgtgcggatc cctcgtcgtc aaagctcctt
     1381 tgattggttt gtccacgggc gccgttcctg ccccagttca gaatgagacg gtttccatcg
     1441 gtagccgctt cccagaagac gtcatcggtc aaccggacga gacggtggaa agacaggtcc
     1501 gggttgagcc cggccgcccc cacgacaccg gtcgaacctc cggcgcctgc cggccccatg
     1561 ccccctatca tcccgcgggg cttttcggtc gactgatgcc ccaggatctt caatatctgt
     1621 tccatgacct tttgcggctg atgcagccaa tccttggcat acaccggaag gaccttccat
     1681 ccgaagcttc gtagcgcagc cggtcgcaaa tagtattgct ctatccggtt gtcattgcga
     1741 tagtggtctt catcgtcgat aagaagagcc aatgcgtatt cctcgtcctc ccctttcttt
     1801 ttcactgcaa gcgaacattt gaaatcggat tgaccgattg gcccttccac ctcataacca
     1861 tgtgcaagaa gctgctcctt cagctgctgc cggaccagcg tagccttcac cgcgtgacca
     1921 tcgggatccc gatgatcgat caatccgtcc aaaatggacc gggccgcggt catacgcccc
     1981 ccgctgacat gttcggcata ctgcaggaat cgccgtagat aattggctcc ttcattgtat
     2041 tcattcgtga tctgttcaaa gcgcatgctg ctgatcactg ccatgtgttt tctcgcgcgg
     2101 ctgaaaagga cattcagccg tttttccccg ccttttttat tcacgggccc aaagttcatg
     2161 accatcctgc cacgtctgtc ggcaccgtag caaacgctga taatgataat atcccgttca
     2221 tcgccctgta tattttccag atttttgacg atcaggccgg taaactgatc gttttcggta
     2281 cggttgtatg cttcttccag caatcgcgcg aaatctacat cctcccgggc aaggttttcg
     2341 agagcgtctt cgatcgcatg cccctgctcc aggctgaaag cgacgatacc gatactttcc
     2401 ggcactcttc tccggaggag ctcccttata agatgggcga tatacgctgc ttccccgggg
     2461 ttggtccgtt tttcatagac gctgcctgtt tgcaggtgga agctgatgct tctgtcgaac
     2521 aacgaatcta caccgaaacc ggcatcctgc ggaccggcaa ccgcgatcgg ttccttcgca
     2581 cgatgatgga tggccttatc aggaatggtg agaagcccgc cttcatagaa cgcatgattg
     2641 ctgaagctga tcagggtctc tgagtgactg cggtaatgcc agctgagtaa agtactttcc
     2701 agcttgcgcg caccctgcgc cagcaggctg tcggcatcga tggtcatccg ttcgccctcg
     2761 tcgtcggttt ctatattcct gtcgaggtct tccggatcgt cggcccgggt gccgaagaaa
     2821 tcggtgggag gcatctgttt ctcatcgccg acgatgatgg tttgctggct gcggtacagg
     2881 gccggaaccc cttcctccag cgtgatttgg ctggcttcgt cgaagatcac cacatcgaaa
     2941 tgatcctcgt cgagagggag actgtcactg acgctataag ggctcatcag ccaaacgggt
     3001 ttcagcgcct tgagcaccag cccgctttct ttttccgaca gctcgcgtat gcttttgaaa
     3061 cgcatgcttt ttccaaactc gttctcgagg atcttccggc cttccgcgta tgctttcttg
     3121 aatttctttt gttcttcggt tagctggcca ggagccctgt tggacagctc cacctgctga
     3181 aggaatcgct gacggacgaa agcacggatc aattctgcat tcacttcctg tagcgtggaa
     3241 tagtcttcgc ccagacgttg aacggcattt tccaatgcga tgccgtcgat ccctgcatat
     3301 tcacgatgtg tctgatacaa gtgctgcagc gtctttctgg cgaccgcagc ttccacctgt
     3361 tcgatccgta agggcagctg tcgaagggtc gttttgacac tgtcgggcag acccgcaaat
     3421 ccgcgcaatg caggcagcca ggccggaagt gcatcggcat tgagccttat gttcaacagc
     3481 tggtcgctga tcatggaaag cgggcccgcg ggcggatcct gaaggcaacg gttgaccgca
     3541 ctctccaacc ggagcagcgg ctggtgtact gccgccaatg cggtaacgac gatctctccc
     3601 cgggggtgat gcagcagcca ggccaattcc ggatgtccat tcctgttttg cagcatctcg
     3661 atgctgagac gggtcgtatc gatattgggt cccagggcga agcgttcccg gagttgttgc
     3721 cgggtctgtt cgagtacggc cgccgcctca tattcggttt tcagtaggtg caggagactg
     3781 ctccaggacg gtgtaacggc atgtgcggag agatcataac tttcgcgcag acggcgtttc
     3841 agcttcctgt acgcaccgtt gaaccggccg aaaaaggtcc cttcgagccg ggtggtcaat
     3901 attatcgcgg aatcgaggtc ctgctcgttc agcttgtgtt tccagtggct gttcttttcc
     3961 gacgccatcc ggagggcttc ctgctgccgg cggtaaacga ggaattcttt ttccagctgc
     4021 cttgactcct cactgtcggg gtcggccagg gccatggttc ctgatcgggc caggggatag
     4081 agcagaacgg caaattgcac caggctcttc agccgatcgt gctccttcaa ctgccgcggc
     4141 tcgagaccgc aagggatgag caacgtttct acttcggcga tggatagtaa cgcttcctgc
     4201 aaggcgtttt cgagtgtgcg gtaggggctt tccgacatcg tgacgatggc ctcctgtatg
     4261 cggctgaacg gatgttctga aaaaaccgat cccgagcctg tttcttccat gacggcatcc
     4321 agcatttgaa ggatggggcc cgccgccttc cactctgaat acgagggcag caattcctcc
     4381 tctcccggat cgaggacagg cagcttttcc cgcagtgcaa taatggattc gagaagtgat
     4441 ctaacggtaa tgcccgtgtc tttatccctg acgagcgccg tctcatggaa ctcgcggatc
     4501 agatcgagat gacgcgacat ccggcggagc agggactcgc ggcgttcctt cagaccgggc
     4561 aggtccatcc tgcggtcgat gaagtcttca taggtggtct tgaggttttt gatgaactcg
     4621 cgtttgtcgc cctggctgtc atggatataa caacacagct cttccagacc gacttgtttt
     4681 agacggtgat acaccacatc gagtgcggcc cttttctcac agacgaaaag gatgcttttc
     4741 ccccgcgcgg taaagtcggc gatgagattg gtgatcgtct ggctcttgcc ggtaccgggc
     4801 ggaccctgta tgatatagct gtagccggcc ctgctttgca ggatggccct ggtttgtgtc
     4861 gggtcggcgc tgacgacatg gaaccagtca tcgggtcggt tgccgtcgaa cgggtgttcg
     4921 ggaaaaagcc ggggcaggtc gccaaaaaga gaatcgaagg tagggtgttg catacgctgg
     4981 tcgatcacga gattgtagtc cctgaccagc gacatctttt tgtaatggaa attgcccagg
     5041 accatgttgc agacatcgaa gtcccagtcg taagggttgc tgtcgacatc ggcggtggcg
     5101 gacggttctg cgatctctgg tccggcgatc tccggcgcgg cgatctccgg cgcggggtat
     5161 tgttcggccg gcgtcgtgtc ttcctcggac gtccccgatt ccgggggagg ggccggatct
     5221 tgcggatcgg tctgtcgcga acgggcagcc ttattccggt attttccgac tatttgcgcg
     5281 gcttcctgct gcacgagcgc aatgcgtggt tttccgacat agcgcagcac gataccctgt
     5341 ccggcttctt cgatctggga tttgacaacc cggtaaaatt cctccgggct catttcttcc
     5401 aggtccacga aatcgggtaa ccggatgcca tgcaattccc tgagctcacc ggcgagaacc
     5461 ggatttacct ccgcaatact atccgacacc tttaggatat aatgatcttc tctcggtctg
     5521 cgattctttt taagcgtcac cggaaccagc aggagcgggc tccggatgct ctcccgggga
     5581 tcatctttaa gattccgcca ttcgagaaag gcgatcacca gcttcagctg gctgaatcca
     5641 tattcctgca tatcccgctg ggcttctact cttaccttat cgagcgatgc ggggagataa
     5701 ggatgatcgt cgaaacgcag gtatttattg agcacgacct cgtcgcagcc gacgatcagt
     5761 cgccccagct cgggactcca ggtaaaaaga aggtccggcc ggatggagcg atagtgcagg
     5821 acgagcggta tactgccgac agtaagattg acgaatcggg catttggctt gaagtatagc
     5881 agccggttcc gacggctggt gtcgaatagc cggttgcgca gccggttgag gatatacgca
     5941 tcccgttctt tcagatccct gtccagccaa ccgggaaggg cgctcagatc ggcctgtttt
     6001 tcaggatcat aatcccggta atgttccagc cgggcgatcg catcgtagag atcctgcgaa
     6061 cggcggcttc gatcgggttc cgtcatttcc gtgatcagac gtccgagggt agggtggatg
     6121 cggggcacat cctgtgtcgg atgctgccgc agttttataa aacaatcgag gtcgttcggg
     6181 tcataaagat cgagacccat ggctatcgac gccaggacga gcccgagaca gaaaatgtcg
     6241 gtttgcggat catggtgacc cagatcgtgc tcgaaacacc ggtaaccggc gagatagaac
     6301 gcttgctccg actgtccggc cgtctcaaat ggcccagcgt gcgggaacag cgcctgaacg
     6361 cgatacgggg cattgcaagg cgcgtgcgcc atcgattcgt cgatatccgc gacgccgtct
     6421 gttacgaaga tcgcctcttg tttttcaaaa ggccccacca gcccggcttc gtgcagcgcc
     6481 aatacttttt tgaatagagg taagacgaag gcgatggtat cgtcggagga cagggaatct
     6541 ttgaaaagga tgcggttcgg ttccatagat aatagttacg gacaaactta cagggattta
     6601 actaacttcg caacatgcga gattatatca gtgaattgaa cgagcagcag agagaagcgg
     6661 tattgcacaa agacggtccg atcatgatcg tggcgggcgc cggaagtggc aagaccaagg
     6721 tcctgacgac acgtatcgcc catctgctgg cccagggcgt cagcgcgttc aatattttgg
     6781 cgctgacctt caccaataag gcggcgaaag agatgaaaga aagggtagag aagatactgg
     6841 gaaacaacga agcacgcagc ctgtatatcg gcactttcca cagcgttttt gcacgtatcc
     6901 tcaggggcga agcgcacctt ctcgatggat atgatcgcaa tttcaccatt tatgacaccg
     6961 acgatgcaaa aagcgtgctg aaaacggtga tcaatgagtt aaatctggac gacaaacact
     7021 ataaaccgaa catggtctac aaccgcatct ccgcggctaa gaacagcctg gccggtcccg
     7081 aagaatatgc gaacgactat ggtatccagc aggaggatat gcgtgccaac cggccggcga
     7141 tcgcgcagat ctacaaagcc tatgtggatc gttgtaagaa gaatggcgcg atggacttcg
     7201 acgatctcct gatgaaaatg tacgaattgc tgaagaagtt tccggagagc cttcacaaat
     7261 tccagcacaa gttcaaatac atccttatcg acgagtacca ggatacgaac cccgcgcagt
     7321 acgagatcat taaattgttg ggcgccgcgc atgagaacgt ctgcgtcgtc ggcgacgacg
     7381 cgcagagcat ctatagcttc aggggagcga ccatcgagaa cattcttcaa ttccagaagg
     7441 actatgatga cgtgaaggtg gtcaagctgg agcagaacta ccgcagcacg cagagcattc
     7501 tgaacgtagc caacgaggtc atccgcaaca acaaggggca gatccccaaa aacctgtgga
     7561 ccgaaaatgc tcccggcgaa aagatccggc tggtacggct gatgacggat aatgacgaag
     7621 gccggttcgt agccgacacc atacaggaac aacggctgcg caatcattac aataatcgcg
     7681 acttcgcgat cctctatcgt accaacgcgc agagccgtgc attcgaagaa agcctccgcc
     7741 ggatgggcat cccgtacacc atgtacggag gcatcagctt ttaccagcgg aaagagatca
     7801 aggatttcat cggttatctg cgtatcatcg tcaatccccg ggatgaggag gcgttgaaac
     7861 gcatcataaa ttatcctgcg cgcggcatcg gcaagacctc tatcgacaga gccgtccttg
     7921 cagccaacga gaacaatatt tcgctgtggg aggttttcga aagggcttcc gaattcggac
     7981 tcagggccgg cacgctggag gcggtcgaca acttcgtagt gatgatccgc agcttcaaat
     8041 ccatgctggc cacgaagaat gcgtatgaag tggccgtcca tgtgggtaaa caaacgaatc
     8101 tcgtcaaaga gctgttcaat gataagagca cggaaggggt cgcccggtac gagaatatcc
     8161 aggaattgct caactcgatc aaggaattta ccgagcgtcc cgatgacgac ggcgtcctcg
     8221 aatccgagac caagggactg ggcgactatc tgcagcagat aacgttgttg actgacgcgg
     8281 acgagaagga tccaaatgcg gatacggtca agatgatgag tatccatgcg gcgaagggtc
     8341 ttgaattctc ctgcgtcttc gcggcgggcc tggaagaaat gcttttcccc aatgcaatgg
     8401 cgatcaatac ccgggaagag ctggaagaag agagaagact cttttatgtc gtgatcacgc
     8461 gtgcaaaaca aaggctctgg atcacatatg ccaatacgcg gtatcgtttc ggcaacctgg
     8521 tgcagaacga gcccagccgt ttcatcgagg agataccttc cgaatacatg gatcgcagct
     8581 ttgccggcgg aggagtccgc aaccagggtt tcggcggagg cccttcggga tccgcttacg
     8641 accggatgca tggcggaggc ttcggctcat ctgcccaaca ggccgagaaa agatatggcc
     8701 cccctccggc aaagaaagaa gatgcccgtc cttcctacgt cccccccaag ccatacatga
     8761 aaacgatcga acacactcca tcgccggatt tcgtcgccag cgatacttcc ggtttgcagg
     8821 tcgggcaaaa ggtcgagcat cagaaattcg ggttcggcga ggtcatcggc atggagggtt
     8881 ccgcgcataa tcccatcgcc accgtcaaat tccagtacaa cggagaaaaa aagatcatgc
     8941 tcaacttcgc gaaattgcgg atcgtagaat gagataatca cgaaaaacat gtcgtaggat
     9001 gcgtcaggca cacgggacga tcgcggaaaa tatcccccgc gcggaaactt tctacgagtg
     9061 ggaaaatcgc ccccgatttt cccacggccg aataaaaaat atccttataa caggctgata
     9121 attaggaagt agttattggg tttcgcaaaa gtttgctatc ttttcgcatg accgaccttt
     9181 acaaggtaca accgcaggga ttgtattttg taaccctaac cgtagttggc gggatcgatg
     9241 ttttcacccg tcacgaatat tgcgatctgc ttgtggataa tctaaactac agcatcgata
     9301 acaaacggtt acgggtatac gaatactcga tcctgcccag ccagctgtac atcatcgccg
     9361 acgtcgaaga aggtaaaagc aatttgccga aagtattgag ggatttcaag agttactcgg
     9421 ccaa
//
LOCUS       DASYSV010000310         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_316068, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000310 DASYSV010000000
VERSION     DASYSV010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316068"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..3078
                     /locus_tag="VGQ51_09260"
     CDS             421..3078
                     /locus_tag="VGQ51_09260"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV9036798.1"
                     /translation="MQLSKNYTPSDTEGKWNDHWEKQRYFNSRPDGRPSFTVVIPPPN
                     VTGVLHMGHTLNETVQDILIRNARMRGYNACWVPGSDHASIATEARVVGMLKEKGIEK
                     SQLTRPEFMKYAYEWKDKYGGIIYDQIRRLGCSVDWERVAFTMDADYYASVIKVFVSL
                     YEQGLIYRGARMIHWDPQAKTALSDEEVEYRDEQGRLYYVDYAIVDKSGRPTGRKVTI
                     ATQRPETIMGDTAICVNPSDPRYTDLSGLFAVVPLVHRQIPIIFDEYVDPAFGTGALK
                     VTPAHDINDYNLGLKHGLPIIDTLNEDGTLSPAAGVFIGMERFAARNAVIKQLKVEGL
                     INKEEDHLTRLGYSQRTHAVVEPRISTQWFVRMKALAQPALKAVTEGYIHIHPGDKFM
                     ATYKYWLENVEDWCISRQLWWGQQIPAWYAPDGTFRVAATRDEAYRLFSAPDGSRPPF
                     GAGQLRQDGDVLDTWFSSWLWPIEVFKGITNPGNADIRYYYPTSVLVTGQDIIFFWVA
                     RMIMAGLWYSPESLPMEKRIPFRHVYFTGMVRDKQGRKMSKSLGNSPDLLALIEKYGA
                     DAVRFGIMIASPAGNDLLFDEAALEQGRNFNNKLWNAMKLVKGWETRLAASGDPTPFP
                     VRWFENRLREARATIEQLFAEFRLSEALKTLYSLIWDDFCSWYLEWVKPGFEQPVDAM
                     VYRRTVGFFEQLMQLLHPFMPFISEEIYHLLADRPEGDDLCVKQFVTEGAADADILAE
                     GQLLKDVITALRDARNKAQLKPKEQVDLYIQTGLKSTYEHIGPILCKQVNASGICMPD
                     TLPGGTLTVVLGKDKFAMVTARILDDDKQKEEMRKELQYLKGFLESVNKKLSNERFVQ
                     NAKPEVVDLERKKKDDAEAKIKILEESLT"
     gene            complement(3075..>4121)
                     /locus_tag="VGQ51_09265"
     CDS             complement(3075..>4121)
                     /locus_tag="VGQ51_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036799.1"
                     /translation="TSYPITSHLINFHSWRPYVNDPDYQLSLVSDNILNTFETDIFAG
                     YNRNEQYKQVGISATYGGLYPLLDAGWNYTIDRNALYRDQTVSWNETEARAGFSIPLG
                     WTRGTGFTSLQFGSDIVYNQRYYTGLYKDSFNSRGFAYIDPYISLVHQSQQAYQQVAP
                     RWAQVLSLSYNSAVTSFTAHQLLATGFLYLPGVAYTHSLVLSGSFQQRDTLTNARFGN
                     SFPFSRGYTAENFYRMWKASANYQLPLLYPDWGFGDILYFLRIRANLYYDYTHAMDFY
                     QGQPVSANFRSAGAEIYFDTKWWNQLALSFGIRYSRLFDTDPGRRGPDQWELILPLNI
                     LSQGYSGRAVRAVD"
BASE COUNT         1012 a         1136 c         1066 g          907 t
ORIGIN      
        1 gatttggaga tatgaaagaa tatattaatt ttgtggctat caattcccac ccctgagaaa
       61 ccactccgtc gaatcaaatt tccagttccc tttgaattcc gcccattaat caaatttggt
      121 tcaccctaaa atttcttact atgaagagga tgtttcaatc tatccatctc ggagaaacag
      181 cgaaaactat tctgctggcg gtaggtttta tcggactggt aatgttcctg tcttatcttt
      241 actggttcgc ttaaatctcc actgatcgga taaaacttta cacttcaaac atattttatg
      301 gcaattcccc atataggtgg ggaatttttt tttcatcccc aacggctgga ccctctcgtc
      361 cccatgcctt gaaaattact acctttgcat ccccgctttg aggacacaat actgtattac
      421 atgcaattga gcaagaatta taccccgtcg gacactgaag gcaaatggaa tgaccattgg
      481 gagaagcaac ggtatttcaa cagccggccg gatggccgtc cctcctttac agtcgtgatc
      541 ccgcctccca atgtcacggg cgtcctgcat atggggcaca ccctcaatga aacggtacag
      601 gacattctta tccgcaacgc cagaatgcgc ggctataacg cctgctgggt ccccggcagc
      661 gaccacgcat ccatcgccac cgaagccagg gtggtcggta tgctaaagga aaagggcatc
      721 gaaaaaagcc agctgacgcg gcccgaattc atgaaatatg cctacgagtg gaaggacaaa
      781 tacggcggca tcatctacga ccagatccgc cgtctcgggt gtagcgtcga ctgggaacgc
      841 gtggccttta cgatggatgc cgattactat gcgtccgtta tcaaggtatt cgtttcgcta
      901 tacgaacagg gactgatcta tcgcggggcc cgcatgatcc attgggaccc acaggcaaag
      961 accgccctca gcgacgagga ggtagaatac cgggacgagc agggccggct atattatgtc
     1021 gactatgcga tcgtggacaa atcgggccgc cccacgggca ggaaggtcac gatcgccacg
     1081 caacgtccgg agaccatcat gggcgataca gccatctgtg tcaacccttc ggaccccaga
     1141 tataccgatc tgtctggtct ttttgcggtg gttcccctgg tacatcggca aatacccatc
     1201 atcttcgatg aatatgtcga tccagccttc ggtacgggcg ccctcaaagt gaccccggca
     1261 catgatatca atgactataa tttgggcctt aaacacgggc tgcccattat cgatacgctg
     1321 aatgaggatg gcaccctgag ccccgcggca ggcgtattta tcgggatgga gagattcgcc
     1381 gcacgaaatg cggtgattaa gcaactaaag gtcgaaggct taatcaataa ggaagaagac
     1441 catctgaccc gtctcggtta ttcccaacgt acccatgccg ttgtcgaacc gcgcatttcg
     1501 acccaatggt tcgtccgcat gaaggccctt gcccaaccgg cgctaaaagc cgtaaccgaa
     1561 ggctatatcc atatccaccc cggcgataag ttcatggcca cctataaata ctggctcgag
     1621 aacgtcgaag actggtgtat ctcccgccag ctgtggtggg gacaacagat acctgcgtgg
     1681 tatgcccccg acggcacctt ccgcgtggcc gccacccgtg atgaggccta tcgcctgttt
     1741 tccgcccccg acggcagccg tccgcctttc ggcgccggcc agcttcgtca ggacggtgac
     1801 gttctcgaca cctggttctc ctcctggctc tggcccatcg aagtcttcaa aggcattacc
     1861 aaccccggca atgccgatat ccgctactat tacccgactt ccgttctcgt cacggggcag
     1921 gatatcatat tcttctgggt ggcgcgcatg atcatggccg gtctctggta ttcccccgaa
     1981 agcctgccga tggaaaaacg catccccttc cgccatgtat atttcacggg tatggtccgc
     2041 gacaaacagg gacgcaagat gagcaagagc ctcggcaact cccccgacct cctggcactt
     2101 atcgaaaaat atggcgccga cgcggtccgc ttcggtatca tgatcgcctc tcccgccggc
     2161 aatgaccttc ttttcgacga ggccgcgctc gaacagggca ggaatttcaa taacaagctg
     2221 tggaatgcga tgaagctcgt gaagggttgg gaaactcgtc tggccgcctc cggcgatccc
     2281 acacctttcc ccgtccggtg gttcgaaaat cgtcttcgcg aggcgcgggc tacgatcgaa
     2341 cagctgtttg ccgaattccg tctaagcgag gcgcttaaga cgctatactc gctgatatgg
     2401 gacgatttct gcagctggta cctggagtgg gtaaaacccg gttttgaaca gcccgtcgat
     2461 gctatggtct atcggcgcac cgtgggattc ttcgaacagc tgatgcagct gctccatccg
     2521 ttcatgccct tcatctccga agagatctat catctgctgg ccgaccgccc ggaaggcgat
     2581 gatctctgtg taaaacaatt tgtaaccgaa ggcgcagccg acgcagacat actggccgaa
     2641 ggacagctgt tgaaggatgt gatcaccgcg ctccgcgatg cccgcaataa ggcacaatta
     2701 aaaccgaaag aacaggtcga cctatacatt caaaccgggc tgaaatctac atatgagcat
     2761 atcggcccca ttctttgcaa gcaggtaaat gcaagcggca tttgcatgcc cgacacactc
     2821 ccggggggga cgttgactgt tgtcctgggt aaagacaagt ttgccatggt tactgccagg
     2881 atactggacg atgataagca gaaagaagaa atgcgtaaag aattgcagta tctcaagggc
     2941 tttttggaat ctgtcaataa gaaactcagc aacgaacgct tcgttcaaaa tgcgaagccc
     3001 gaagtggtag accttgagcg aaagaagaag gatgatgccg aagcgaagat caaaatatta
     3061 gaagaaagtc tcacctaatc cactgcccgc accgcgcgcc cgctataccc ctgcgacaat
     3121 atgttcaacg gcaggatcag ctcccattga tcgggccccc gccttccggg gtctgtgtcg
     3181 aacaaccggc tgtagcgtat cccgaaagac agcgccagct ggttccacca tttcgtatcg
     3241 aaatagatct cggcgcccgc gctgcgaaaa ttggcgctga ccggctgccc ctggtagaaa
     3301 tccatcgcat gcgtatagtc gtaatagaga ttggcccgga tacgcaggaa ataaaggatg
     3361 tccccgaaac cccagtcggg atacaacagc ggcagctgat agttcgccga agccttccac
     3421 atccggtaaa aattttcggc ggtataaccc cttgagaagg gaaagctgtt gccgaaccgc
     3481 gcgttggtca gcgtatcgcg ttgctgaaag gagccggata gcaccaggct atgggtgtac
     3541 gccacacccg gaagatacag gaacccggta gccagcagct gatgggccgt gaacgaagtc
     3601 acggcgctgt tatatgacag gctcaatacc tgcgcccatc gcggggctac ctgctgatag
     3661 gcctgctgag attgatggac caggctgata tagggatcga tataggcgaa cccgcggcta
     3721 ttgaaggagt ctttgtacag cccggtgtaa taacgctgat tgtagacgat gtcggacccg
     3781 aactgaaggc tggtaaaacc agtgccccgc gtccagccga gcggaatgct gaaacctgcc
     3841 cgggcctccg tctcattcca ggataccgtc tgatcgcgat acaacgcatt ccggtcgatg
     3901 gtataattcc atccggcgtc cagcaaagga tacaacccgc cataggtggc gcttatccct
     3961 acctgtttgt attgctcgtt gcggttatac ccggcgaaaa tatcggtctc gaaggtgttg
     4021 aggatattgt cgctgacaag cgatagctga tagtcgggat cattgacata aggacgccag
     4081 ctgtgaaaat tgatgagatg cgaggtgatg ggataactgg t
//
LOCUS       DASYSV010000311        12733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1212494, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000311 DASYSV010000000
VERSION     DASYSV010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212494"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2666)
                     /locus_tag="VGQ51_09270"
     CDS             complement(<1..2666)
                     /locus_tag="VGQ51_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEV9036800.1"
                     /translation="MLSTVMVSAQPVREVRLTQFTLQSSAVVRAGGDSISSVGYSPAN
                     YWFPVNVPSTVLTGLVANKVYPDPYIGMNNMLIPDASDNFNHRYNLEQYSYLPGQSNP
                     WKKPYWYRTTFSVPGADKGRHFQLVFKGINYRAEVWLNGHLIADSSKMVGMFQEFDLD
                     VSSAIRAGGENALAVRIYPLDVPGEPATPQLKALGDFYENGGPTGDIGKNVTMLSSVG
                     WDWIPEVRDRNMGIWQPVYLRTTGQVTINRPRIVTELPDLPDTTVARLSLDVLLTNHD
                     SQTRRGTLQVTITPENFTGPAIHFTREEAVGASEAKTIRLSPSDVAELTMRHPRLWWP
                     NGYGHPWLYRIRLQYVSGGGISDDTSFVFGVRTVSSAATTVNGWVRRDFFVNGRRVHL
                     AGGAWVPDMMLNRDRQRYDYELRLCRNANVNLVRIWGGGLGETDDFYELADRYGLLVW
                     QDFWITGDTNGGFKGSSDWPLETHVFVDNVISTIYRIRNHPSLLVWTGGNEGHAREEL
                     YNAMRDNVAALDGTRPFIPCSSGFSKAPADWKGSWPDDKPAGVYSGGPYSWQDDARYY
                     RLVDSGRDWVFKDETGLPSQPPYNTLARIIPNLVPDNNLPYPLNDTWGYHDACTGNGH
                     YDTYYQAMLNRFGAPVSMRDFSQKMQLLNAGGYRSIFEAAGHKLNETGGVMLWKLNAA
                     FPSVVWQIYDWYLEPNAGYYFMQRACEPVHIQLNLDDSTVALINRTYVRRPGLHYDVQ
                     VIGMTGASLFHRQGNASLDTTDVKKVLPLKDVLASATGVSFVLLRLTDDKGAVLSQNV
                     YWMSPGHDFADLHSMPSSSLTVSVVSSDRRNGYYNWTIRFTNTTSRLAFFLNPQVIAG
                     GEEVLPSFWSDNYFSVPAGQSVTVHVSCPGSML"
     gene            complement(2776..4272)
                     /locus_tag="VGQ51_09275"
     CDS             complement(2776..4272)
                     /locus_tag="VGQ51_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter TolR"
                     /protein_id="HEV9036801.1"
                     /translation="MRYQFVLLLLLSARCVFAQPIGQFDGHGDVGTNVKPGSALYIPA
                     TGQYVVSGAGYNIWGDHDEFQYVWKKMKGDFILYTRAEFLGGWVDYHRKIGWMIRKSL
                     DGNSPHVNAVVHGDGLTSLQWRPAAGAQTQEHRLRLSKVNILQLERKGDRYIMRAAVY
                     GEPFQTDTLTGVDLGDEVYVGLFVGSHNADVLETGVFSNVSIAVPYKSEANQSTQMTL
                     GSRLEILQIASGHRDVIYEVPYSIQAPNWLPDGQHLIFNDAKGLVYKFDLSTRTPTPL
                     NTGAVMSNNNDHVISFDGRMLGLSSWVRDLGGSIIYTVPIGGGTPKQITPKGPSYLHG
                     WSPDGKDLVFCGERNGEFDVYKVPAEGGKEIRLTTTQGLDDGPEYTPDGRYIYFNSVR
                     SGLMQIWRMRPDGSDQEQVTNDDYNNWFAHISPDGKWFVYLSFLKEETPASIHPPYKH
                     VYIRLLPIDGRGTPKVLAYLYGGQGSINTPSWSPDGKRIAFISNTAAN"
     gene            4321..5433
                     /locus_tag="VGQ51_09280"
     CDS             4321..5433
                     /locus_tag="VGQ51_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV9036802.1"
                     /translation="MKIAILLAGLLFFSGASHNDEHPTLPIGATAPDFSLTGVDGKTW
                     SLSSFRDAKVLVIVFTCNHCPTAQAYEDRIMALAKDYKDKGVALVAIMPNDPGSIRLD
                     ELGYTDMGDSFDEMKRRASTKHFNFPYLYDGATEEASKKYGPIATPHVFVFDRERKLR
                     YSGRVDDVEKPTKIPHSSDARNAIEDLLAGKPVSVTKTKVFGCSIKWAEKSDWIDKAK
                     KEWAAEPVHLDTITEAGLRELVANHTDKLLLINVWATWCGPCVAEFPDLIDINRMYRR
                     RDFEFISISADEPAKRAKALEFLDKTQASGRNFIFNIDDKYKLIEAIDPKWQGALPYT
                     LLVAPGGKIVYSKEGPIDPQALKTAIVDDPAIGRYY"
     gene            5479..6876
                     /locus_tag="VGQ51_09285"
     CDS             5479..6876
                     /locus_tag="VGQ51_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV9036803.1"
                     /translation="MSSSNRKGTTFSGIALVLRQALSGEHRDYTQGSIRRSVILLSIP
                     MILEMCMESLFAVVDIYFVGRLGKEAVAAAALTESVLAIVYSLAMGLAMAATAMIARR
                     VGEKNEKEASHTAAQAILITLGLTVLVSVAGAMLAPQILGVMGASPTAVRIGQSFTRI
                     IFESSIVIMLLFLINGIFRGAGNAAMAMWSLWIANGFNILLCPILVHYYGLPGAAMAT
                     TIGRGIGVCYQLYHLVRGRGLIRIIARHFKPDSAIMRGLAKIAWTGAMQFLISTASWI
                     AMARIIASFHDTAIAGYQVSIRIIIFFILPAWGMSNAAATLVGQNLGAGQPERAVASV
                     RTAALYNALFMSLVSLLFLVAAEPIVDFLNPDPNVQAIAVRSLHIISLGYVFYGVGMV
                     ITNTFNGAGDTRTPTIISLFIFWAFQIPLAWFLAIFLGQGPTGVFIAIVVAESSLSVV
                     SYLIFRQGKWKLVKV"
     gene            7038..7760
                     /locus_tag="VGQ51_09290"
     CDS             7038..7760
                     /locus_tag="VGQ51_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEV9036804.1"
                     /translation="MRRRILHPGFLCVLVAFVLPVNVLIAGGPVNAKKAAKAAAFAVE
                     VNNLYTQIDLKELGLTKKAFEYALKGYYYLLDHHWLNKTNILSICDLSQSSRNKRLYI
                     VDLDTKTVLVNTYVAHGRNSGGEFARTFSNNSSSHKSSLGFYVTQGTYYGNNGLSLRI
                     RGMERGFNDRAISRGVVVHGSEYVGPDFLEMNRFCGRSYGCPAVPADESETIIDLIKE
                     GSCLFIYYPTKKYLSRSKILNS"
     gene            complement(7854..8519)
                     /locus_tag="VGQ51_09295"
     CDS             complement(7854..8519)
                     /locus_tag="VGQ51_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036805.1"
                     /translation="MTDTTVAARTDTPVAASPIIRILAKGITYLFHPLFITTYVMGFL
                     IFFHPFAFVSFDHKDKVLKFINIVFCDAFLPLFSVFLMWRLQFVQSMLVRSQKDRTIP
                     YMMAMIFYWWTWHLYQNWEEVPVSAIHFLLGAFLAICVAFFCNIFFKISMHSVAMGGA
                     LMFFVLFGFTDTYASGLYIAVALLLTGLVCSSRLILAEHSGFEVWTGLLAGMVTQLVA
                     WQF"
     gene            complement(8516..9997)
                     /gene="rpoN"
                     /locus_tag="VGQ51_09300"
     CDS             complement(8516..9997)
                     /gene="rpoN"
                     /locus_tag="VGQ51_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEV9036806.1"
                     /translation="MALSQSLQQKLLQKLSPQQIQLMKLLQVPTANLEERIKEELEEN
                     PALEVTEDHEDGLGEESKEEFESEEYDEKDGSEDEYDNIDISEYVNDSDDDVADYKLR
                     DDNYPDQDEDRVMPHRVETSFHELLLDQLGMLVVDDRTRKIAEQIVGSIDDDGYLRRE
                     TASIADDLAFRQNIETTEEEIDGLIKKIQQFDPPGVAARDLQECLLIQLHRKKNGHKS
                     VDVAIDVLTYYFDEFTKKHYDKIQRGLNLTDDSLKEVINQIIKLNPKPGGDVTEVNKA
                     ESYVIPDFFIANTGGKLELSLNSKNAPDLRISEGYKEMLKEYDRGSKKDKRQKEAVLF
                     IKQKIDAAKWFIDAIKQRQQTLYNTMHTIMDYQRDFFLTGDETTLRPMILKDIAERTA
                     LDISTVSRVANSKFVQTEFGTYRLKFFFSESLSTDTGEEVSTREVKKILSDLIEGESK
                     KKPLSDEKLTELLQERGYNIARRTVAKYREQLNIPVARLRKEL"
     gene            10185..10391
                     /locus_tag="VGQ51_09305"
     CDS             10185..10391
                     /locus_tag="VGQ51_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036807.1"
                     /translation="MDKFIPDHLEFKRMFVMPIKDTFPSVRFTAGPSLGFFCKQELVI
                     QKALRLPIFFRLGSLEYCNKLEGK"
     gene            10391..11449
                     /locus_tag="VGQ51_09310"
     CDS             10391..11449
                     /locus_tag="VGQ51_09310"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV9036808.1"
                     /translation="MTKPYYPGLSGLRAIAAFLVLFTHVEEMRQLRGMKGLVDPGFNA
                     FLGGVAVTFFFVLSGLLITGLLLTEKKQSGTIRMKRFFINRALRIWPLYYLVLFAGYG
                     ISIFLFHETDTDIFTNGMLLGSLLLPNVAFASGLLPKILVQLWSIGPEEQFYFSWPFL
                     IRRLRPQTLACVFIGIILFWAAAIGALRVFYGPDTWLHALLFRTRIDCMAIGGLSALL
                     LFYHGATDGRRTAIYRLFQRRETGWVAAALFTGLLVISYHYHVSLYQLYAALSALLIL
                     RVMTRPAGWLEMPSLRYLGRISYGVYLLHPFAIYLIFSFVKGGPAVLFFLASAALTVG
                     LAALSYRFYESRFLKRKQ"
     gene            complement(11453..>12733)
                     /locus_tag="VGQ51_09315"
     CDS             complement(11453..>12733)
                     /locus_tag="VGQ51_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEV9036809.1"
                     /translation="ILTAAIMAFYFFYEAFIQWMTRHFPHRFFTTNGFLLSFGLGLLI
                     AVFLVVKKGRGTFSRLYMFLNVLLIIYIVTDAGGLIVKALHPPVNPLSVKDEGTPPCG
                     TCAKPNVYFLLFDEYAGSLALKEQYHFDNDLDSFLLRQGFHINGRSRSNYNLTPFSMA
                     SVLNMAYIEGIKEPLHIVSQDYTNTSQLIRDSRVVRIFSAEGYAIKNYSIFDLAGHPA
                     PAWQSFLPLKSRLITARTMGQRLERHWNSLVIRDLGIRHFVVQDYMKYAASNEEFLTA
                     VASEADHNDGRPKFIYAHFMMPHFPYLFDSLGRRKSDREIYEERSTNPSSAYLGYVRY
                     TNRRLEPLLASIRRNDPGAVIILCGDHGYRGWANRQNPTLQFHNLNAVYFPDHDYSRW
                     NDSTAFVNQFRIVFDQLFHAEFPLLRDSTVLLSE"
BASE COUNT         2900 a         3355 c         3421 g         3057 t
ORIGIN      
        1 agcatcgaac caggacagct gacatgtacc gtgacagact gccccgccgg aacagagaaa
       61 taattatcgg accaaaagct tggcaatacc tcctctccac cggcgatgac ctgcggattg
      121 aggaaaaacg cgagacgcga agtggtgttt gtgaaccgga tcgtccaatt gtaatagcca
      181 ttcctgcgat ccgacgaaac cacggacacc gtcaatgatg atgaaggcat gctgtgcaga
      241 tcggcgaagt catgaccggg agacatccaa tagacattct gcgagaggac agcgcccttg
      301 tcatcggtga gccgcagcaa aacaaacgat accccggtcg ccgaagcaag cacatccttc
      361 agcggaagga cctttttaac atctgtggtg tcgagcgatg cgttaccctg ccggtggaaa
      421 agcgacgcgc ctgtcatgcc gatcacctga acatcgtagt gaagtcccgg ccgccgcaca
      481 taggtccggt tgatgagcgc cacagtggaa tcatccaggt tgagctggat atgcaccggc
      541 tcgcacgcgc gctgcataaa ataatacccc gcattgggtt cgaggtacca atcgtatatc
      601 tgccagacga cactcggaaa ggctgcattc agcttccaga gcatgacacc gcccgtttca
      661 ttgagcttgt gacctgccgc ttcgaagatc gagcgatagc cgccggcatt caggagctgc
      721 atcttctgcg aaaaatcgcg catgctgacg ggcgcgccga acctgttcag catggcttga
      781 tagtaagtgt cgtagtgtcc gttgccggta caggcatcat gataacccca ggtatcgttc
      841 agagggtaag gaagattgtt atcgggtacg agattgggaa tgatcctcgc caatgtattg
      901 taaggcggct gtgaaggcag gccggtctca tccttaaaga cccaatccct gccggagtcg
      961 accagccggt aatatcgtgc atcgtcctgc caggaatacg gccccccgct ataaacgcct
     1021 gcaggcttgt catcaggcca ggagcctttc cagtcggcgg gcgccttgga aaaaccggat
     1081 gaacagggta tgaacggtcg ggtcccgtcc agcgccgcga cattatcacg catggcattg
     1141 tacagctctt cccgggcatg cccttcatta cctcccgtcc acaccagcag tgacggatga
     1201 ttccggatac gatagatagt gctgatcaca ttatcgacaa agacatgcgt ctccaacggc
     1261 cagtccgaag agcctttaaa accgccattg gtatcgccgg tgatccagaa atcctgccag
     1321 acgagcaggc cataacggtc cgccagctca tagaagtcgt ccgtttcacc caggcctccg
     1381 ccccagatac ggacgaggtt gacattggcg ttacggcaaa gacgaagctc atagtcatag
     1441 cgttgacgat ccctgttgag catcatatcg ggcacccagg ctccccctgc caggtgaact
     1501 ctcctgccat tgacaaaaaa gtcgcggcgg acccagccat tgacggtcgt cgccgccgaa
     1561 cttaccgttc gcacaccgaa cacgaaagag gtgtcgtcgg aaatgccgcc tccgctgacg
     1621 tattgaagcc ggatacggta aagccaggga tgcccataac cgttaggcca ccagagacgg
     1681 ggatgacgca tcgtcagttc ggcaacatct gacggactta gccggatggt cttcgcctca
     1741 gacgcgccga cagcttcttc gcgggtaaaa tgaatggcgg gcccggtaaa attctccggg
     1801 gtaatggtta cctgcagcgt tccgcgacgc gtctgcgagt catgattcgt caacagcaca
     1861 tcgagcgaga gccgcgcgac agtcgtatcc ggaagatcgg gcagttcggt cacgatacgc
     1921 ggccggttga tagtaacctg tcccgtcgtg cggagataca caggctgcca gatccccata
     1981 ttgcggtcgc ggacttcagg gatccaatcc caacctaccg agctgagcat ggtcacattc
     2041 ttgccaatgt cgccggtggg tcctccattt tcataaaaat cgccaagagc ctttagctgc
     2101 ggcgtggccg gttcgcccgg gacatccagc ggatagatcc tgacggcaag cgcgttctcg
     2161 ccgccggcgc ggatagctga agagacgtcc aggtcgaatt cctggaacat gcccaccatc
     2221 ttcgatgaat cggcgatcag atggccgttt agccatactt ccgcgcggta gttgatgcct
     2281 ttgaatacca gctggaaatg acggcccttg tcggcgccgg gtactgaaaa cgtggtgcgg
     2341 taccaatatg gctttttcca gggattcgac tgcccgggaa gatagctgta ttgttcgagg
     2401 ttataccggt gattgaagtt gtcggatgca tccgggatca gcatgttgtt cattccgata
     2461 taaggatccg gatagacctt attggctacg agtccggtca gcacggtgga ggggacattg
     2521 accgggaacc agtagttcgc gggcgaatag ccgacggaag atatcgaatc gcctcctgca
     2581 cgaacgacgg cggaggattg cagggtaaac tgggtcagcc tgacctcgcg caccggctgt
     2641 gcggagacca tgacagtgga cagcataagt aaaaaaaaag catatgcacg catcgtagag
     2701 tggcgtttag gtaaaaggtt ggctaaatta gataaaaggt ttgtgaccta ctacctaata
     2761 tacatcactt tctgatcaat ttgcagcggt attgctgatg aaggcgatcc gttttccgtc
     2821 gggcgaccag gacggagtgt tgatggatcc ctgtccgccg tacagataag ccaggacctt
     2881 cggcgttccg cgtccatcga tgggcagcag acggatatag acatgtttgt agggcggatg
     2941 aatactggcc ggcgtttcct ctttcaaaaa gctgagataa acgaaccatt tcccatccgg
     3001 cgaaatatgc gcgaaccagt tgttatagtc gtcattggtc acctgttcct gatcgcttcc
     3061 gtcgggccgc atacgccaga tctgcatcag cccgctgcga actgagttga aatagatata
     3121 ccggccatcg ggggtatatt cggggccgtc gtccagacct tgcgtcgtcg tcagacggat
     3181 ctcttttccg ccttcggcag ggaccttgta cacatcgaac tcgccatttc gctccccaca
     3241 gaataccagg tcctttccat cgggtgacca gccatgcaga taagacgggc ctttaggcgt
     3301 gatctgtttg ggagtgccgc ccccgatcgg cactgtatag atgatcgatc ccccgaggtc
     3361 gcgtacccag ctgctcaaac ccagcatacg tccgtcaaag gagatcacgt gatcgttgtt
     3421 attggacatc accgctccgg tattcagcgg cgtgggagtc cgggtggaca gatcgaattt
     3481 atagacgaga cctttggcat cattgaagat cagatgctga ccatccggca gccagttggg
     3541 ggcctggatg gaatagggga cctcgtatat tacgtcccgg tgtccggagg ctatttgcaa
     3601 tatttccagc ctgctgccca gcgtcatctg cgtcgattgg ttcgcttcac ttttataggg
     3661 caccgcgata ctgacattgc tgaagacccc cgtttccagc acgtcggcat tatgagagcc
     3721 gacgaacagg ccgacataga cctcatcgcc caggtcgacg ccagtgaggg tatcggtctg
     3781 aaatggctcg ccatagacgg ctgcacgcat gatataccgg tcgccttttc tttcgagctg
     3841 gaggatgttg accttgctta accggagccg gtgttcctgc gtctgcgcgc ctgctgccgg
     3901 tcgccactgc aatgacgtca atccgtctcc atggaccacc gcattcacat gcggggaatt
     3961 gccgtcgagg ctcttgcgga tcatccagcc tatcttccgg tgatagtcga cccagccacc
     4021 caggaattcg gcgcgtgtat agagaatgaa gtcgcccttc attttcttcc atacatattg
     4081 aaattcgtcg tggtcgcccc agatgttgta gccggcgccg gagacgacat attgacccgt
     4141 agccgggatg tacagggccg agcctggttt gacattcgtg cctacgtctc catgaccgtc
     4201 gaattggccg atcggctggg cgaagacgca ccgtgccgaa agcagcagca gtaaaacgaa
     4261 ttggtatctc attagaattt tagtgtacta atttacggta actttcataa aatatcccgc
     4321 atgaagatcg caatacttct cgccggcctt ctcttttttt ccggagcaag ccataacgac
     4381 gaacatccca cgctgccgat aggcgccacc gctccggatt tctcgctgac gggggttgat
     4441 ggaaagacct ggtcactttc cagtttcagg gatgcaaagg tgctggtgat cgtatttacc
     4501 tgcaatcatt gtccaaccgc gcaggcctat gaggaccgga tcatggctct cgcaaaggat
     4561 tataaagaca agggagtggc gctggtggcg atcatgccca acgacccggg ctcgatccgt
     4621 ctggacgagc tgggctatac cgatatgggt gacagttttg acgagatgaa gcgtagggcg
     4681 tctaccaaac atttcaattt cccctatctc tatgatggcg ctacggagga ggcttcaaaa
     4741 aaatacggtc ccatcgccac tccgcatgtg ttcgtcttcg atcgggaacg gaagctacgc
     4801 tacagcggcc gtgtcgacga tgtcgagaag ccgacgaaaa tccctcattc gagcgatgcc
     4861 cgcaatgcca tcgaggacct gttagccggg aaaccggtgt ctgtgacgaa gaccaaggtc
     4921 ttcggctgca gtatcaaatg ggcggagaaa agcgattgga tagataaagc caaaaaagaa
     4981 tgggcggcgg aaccggttca cctggatacg atcacggaag ccggtctcag ggagctggtc
     5041 gccaatcata cggacaaatt gcttctgatc aatgtctggg ctacctggtg cggaccctgc
     5101 gtggccgagt ttcccgatct tatcgatatc aaccgcatgt atcgccggag ggatttcgaa
     5161 ttcatttcga tcagcgccga cgaaccggcc aaaagagcaa aggcgttgga gttcctggat
     5221 aagacccagg cctccggccg aaattttatt ttcaatatag acgataaata taagttgata
     5281 gaggcgatcg accccaaatg gcagggagcg ctgccctata cgctgctggt ggcaccgggc
     5341 ggaaagatcg tctactccaa agaaggaccg atcgatccgc aggcattgaa gaccgctatc
     5401 gtcgatgacc cggcgatcgg tagatattat tgagggctgg attcttcatc cgccaaatat
     5461 attaatttag agtcacatat gtcttcgtct aaccgaaagg gaactacttt ttccggcatc
     5521 gcgctggtac tgcgccaggc cttgtcggga gagcacaggg attatactca gggaagcatc
     5581 cggcgttcgg tcatcctcct ttccattccg atgatcctgg agatgtgtat ggagtccctt
     5641 tttgctgtcg tcgatatcta ttttgtaggc cgtctgggca aggaggcggt tgccgccgct
     5701 gcattgacag agtctgtcct tgcgatcgtt tattcgctgg ccatgggact tgctatggcc
     5761 gccacggcaa tgatcgcccg tcgcgtcggc gaaaaaaatg aaaaggaggc atctcatact
     5821 gcagcacagg cgatcctgat aacgcttggt ctgactgtgc tggttagcgt tgccggcgcc
     5881 atgctggcgc cgcagatcct cggggtgatg ggagcctctc ccacagccgt ccggatcgga
     5941 cagtcgttta cccgcattat tttcgaaagc agcatcgtga tcatgttgct ctttctcatc
     6001 aatggcatct ttcggggagc cggcaatgcg gctatggcga tgtggtcgct atggatagcc
     6061 aacggattta acatcctgct ctgtcctatc ctggtccatt attatggact accgggcgcc
     6121 gccatggcca cgaccatcgg cagaggtatc ggggtctgtt accagctgta tcatcttgtc
     6181 cgcggacgcg gcctgatccg gatcatagct cggcatttca agcccgattc ggccatcatg
     6241 cgtggcctgg caaagatcgc ctggacggga gcgatgcaat tcctcatcag cacagccagc
     6301 tggatcgcca tggcccggat catcgcctct ttccatgata cggcaatagc cggctaccag
     6361 gtctcgatac ggataattat cttttttatc cttccagcct gggggatgtc gaacgcagcc
     6421 gcaacccttg tcggccaaaa tctgggggcc ggacagcccg aaagagcggt cgcgtcggtg
     6481 cgcacagcgg ccctttataa tgccttgttt atgagcctcg tatcattatt atttttggtg
     6541 gccgccgagc ccatcgtcga tttcctcaat cccgacccta atgtccaggc cattgccgtc
     6601 cgttcgttgc atattattag cctggggtat gtcttttatg gggttgggat ggtgataacc
     6661 aatacgttca atggagccgg ggatacccgg acccccacca ttatcagcct gttcattttc
     6721 tgggctttcc agatccccct tgcctggttc ctggccatct tcctgggtca gggccctaca
     6781 ggcgtgttta tagccattgt cgtggccgaa tccagcctca gtgtggtcag ttatctcatt
     6841 ttccggcagg gtaaatggaa gctggtgaag gtataagggt ataattttca ggggtagaaa
     6901 accggccggc cacacaataa aaagatttca taaaagactc ttgcacaagt agaaaataga
     6961 taatatttta gatacccggc cgttttaaaa tagcgccagc ttatgagaag caggtcggca
     7021 gataaaacct ttgaaacatg cgtcgtcgta ttctacatcc gggattcctg tgcgttttgg
     7081 tcgcattcgt tttgccggtt aacgtgctga tcgccggtgg cccggtgaat gcaaaaaaag
     7141 cagcgaaggc ggcagccttt gccgtggaag tcaataacct atatacgcag atcgatctga
     7201 aagagctggg gctgaccaaa aaagccttcg agtatgctct taaaggatat tattatctgc
     7261 tcgatcacca ctggctgaac aagacgaata ttttatccat ctgcgatctg agccagtctt
     7321 cgcgtaacaa gcggctatat atcgtcgatc tggatacgaa gaccgtattg gtgaatactt
     7381 atgtggcgca tgggaggaat tccggaggcg aattcgcaag aacgttctcc aacaattctt
     7441 cttcgcataa aagctcgctg ggtttttatg tcacgcaggg gacctattat ggaaacaatg
     7501 ggctgtcgtt gcgtatcagg gggatggaaa gaggtttcaa tgacagggcc atctcgcgcg
     7561 gtgtagtggt gcatggttcc gaatatgtcg gtccggattt cctggagatg aaccggtttt
     7621 gtggaaggag ctacggttgc ccggctgttc ccgccgatga gtcggagacg atcatcgatc
     7681 ttataaaaga aggttcttgt ttatttatct attacccgac caaaaaatac ctaagccgat
     7741 cgaagatact taacagctag catggacgca cacttcagat ggtttgaagg tgaaaaagct
     7801 caatataggt tcgtacccta tggttgggct tttctttttt agcccggacg cggctaaaat
     7861 tgccatgcta ccagttgcgt caccatgccg gcaagcaggc ccgtccatac ctcgaatccc
     7921 gaatgctccg ccaggatcag ccgcgagctg caaacgagcc cggtcagaag caatgcaacg
     7981 gcaatataca agccggacgc ataagtatcg gtaaacccaa aaagtacaaa gaacatcagc
     8041 gccccgccca tggcgaccga atgcatgctg atcttaaaaa agatattgca gaaaaatgcg
     8101 acgcagatcg ccaggaacgc gcccagcaga aagtggatcg ccgagacggg tacctcttcc
     8161 caattctgat acagatgcca ggtccaccaa taaaagatca tagccatcat atagggaatg
     8221 gtcctgtctt tttgacttcg caccagcatc gattggacaa attgcagcct ccacatcaag
     8281 aaaaccgaaa ataatggcag aaatgcatcg caaaaaacga tattgatgaa cttcaacacc
     8341 ttatccttgt ggtcgaacga gacgaaggca aaggggtgga aaaagatcag gaaccccatc
     8401 acatacgtgg taatgaacag cgggtggaag aggtaggtga tcccttttgc cagtatccgt
     8461 attatggggc tggctgcgac cggagtatcg gtcctggcgg ctacagtggt gtcggtcata
     8521 attccttcct gagtctggcg acgggaatat ttaattgttc gcggtatttg gctacggtgc
     8581 ggcgggcaat attgtatccc cgctcctgga gcagctccgt cagcttttca tcgctgagcg
     8641 gtttcttctt gctttcccct tcgatcagat cgctgagtat cttcttcacc tcccttgtcg
     8701 agacctcctc gccggtatcg gtactgagcg attcgctgaa aaagaatttc aaccggtagg
     8761 tgccgaattc ggtctgaacg aacttgctgt tggcaacgcg gctgacggtg gagatatcga
     8821 gggcggtccg ttctgctatg tccttgagga tcatcggacg cagcgtagtc tcgtctcccg
     8881 tcaggaagaa atcgcgctgg tagtccatga tcgtatgcat ggtgttgtag agggtctgct
     8941 gccgttgctt tatcgcatcg atgaaccact tggccgcatc gatcttttgc ttgatgaaga
     9001 gcacggcctc cttctggcgt ttgtccttct tgctgccccg gtcatattcc ttgagcattt
     9061 ccttataccc ttcgctgatt cgcagatcgg gggcattctt ggagttgagc gacaattcca
     9121 gcttcccccc ggtgttggca ataaagaaat ccgggatcac atagctttcg gccttattga
     9181 cctcggtgac atcgcccccc ggcttggggt tgagcttgat gatctggtta attacctctt
     9241 tcagcgaatc gtcggtcaga ttgagaccac gctggatctt atcgtaatgt ttcttggtga
     9301 actcgtcgaa gtagtaggtg aggacgtcga tggctacgtc gacactctta tggccgttct
     9361 ttttccggtg aagctggatc agcaaacatt cctggagatc gcgggcggcg acaccgggcg
     9421 gatcgaactg ctggatcttt tttatcaacc cgtcgatctc ctcttcggtg gtttcgatat
     9481 tctggcggaa tgccaggtca tcagcgatgg aggccgtttc ccgacgcagg tacccgtcgt
     9541 cgtcgatgct accgacgatc tgttcggcga tcttgcgtgt ccggtcgtcg acgaccagca
     9601 tgccgagctg atcaagcaac agctcatgaa aagaggtttc taccctatgt ggcatcaccc
     9661 tgtcttcatc ctgatccggg tagttatcat cccgcaactt gtaatccgct acgtcgtcat
     9721 cggaatcgtt gacatattcg ctgatgtcga tgttgtcata ttcatcctcg ctgccatcct
     9781 tttcatcata ttcttcagat tcgaattctt ccttcgactc ctcgcccagc ccatcttcat
     9841 gatcttccgt cacctcgagc gcagggttct cctccagctc ctccttgatc cgttcttcaa
     9901 ggttggccgt gggtacctgc aacagtttca ttaactgaat ctgctgcgga gacagttttt
     9961 ggagtaattt ttgctgtaat gattgactta aagccatgta aatcaggggt tttgctccta
    10021 tcctttttcc atttagcaaa aaaagggtcg cgaaccacaa aaatcaggcc gtaaatttac
    10081 ataaaaagga gggataccgc gtgcaacgaa aaaaaacttt tcttgttgta tgtttttcaa
    10141 tacttataag ggcgctcccg gcccagaccc cgtccgcgcc caaagtggat aaattcatac
    10201 ccgatcatct tgaattcaag cggatgttcg ttatgccgat aaaggatacc ttcccgtctg
    10261 ttcgcttcac ggccggccct tccctgggtt ttttctgcaa acaagagctg gtgatccaaa
    10321 aggccctccg gctccccatt ttcttccgtc tgggctcact ggaatattgt aataaactgg
    10381 aggggaaata gtgaccaaac cctattatcc gggtctttca ggactaaggg caatagcggc
    10441 cttcttggtc cttttcaccc atgtcgaaga aatgcggcag ttgcggggaa tgaagggtct
    10501 cgtcgatccg ggtttcaacg cttttctcgg gggtgtggcc gttacctttt ttttcgtgct
    10561 gagcgggttg ctgatcacgg gattgctgct gacagagaaa aagcagtctg ggacgatcag
    10621 gatgaaacgc ttcttcatca accgcgccct ccgcatctgg ccgctctatt atctcgtact
    10681 gttcgccggg tatggtatct ccattttcct cttccatgag acggataccg atattttcac
    10741 gaatggcatg ctcctgggct ccctgctcct ccccaatgtc gcatttgctt ccggtctgct
    10801 gccgaagatc ctcgtccagc tctggtccat cggtccggaa gaacagttct atttcagctg
    10861 gcccttcctc atccgccggt tacggccaca aacgctagcc tgcgtcttta ttggaatcat
    10921 cctcttctgg gcggcagcca tcggggcgct tagggtgttt tatggacccg atacctggct
    10981 tcatgcgttg cttttcagga ccaggatcga ttgcatggcg atcggcgggc tgtcggcact
    11041 gttgctgttc tatcacgggg caacggatgg aaggcggacg gctatttacc gtctgtttca
    11101 gcgccgcgag acgggttggg tggcggcggc tctgttcacc gggttgctgg tcatcagcta
    11161 tcactaccac gtcagccttt accagctgta tgccgccctt tccgcgctgt tgatcctgcg
    11221 cgttatgacc cggcccgccg gatggctgga aatgccgtca ttgcgctacc tgggcaggat
    11281 ctcctatggc gtctaccttt tgcacccctt tgcgatctat ttaattttct cttttgtcaa
    11341 aggcggaccg gcagtcctct tctttctggc atcggcagca ctgaccgtgg ggctcgcggc
    11401 actcagctat cgattctatg aaagtcgttt cctgaaaaga aagcaatagt ccttattcgc
    11461 taagcagcac cgtactatcc ctgagcaagg gaaattctgc atggaacagc tggtcgaaga
    11521 cgatccggaa ctggttcacg aacgcggtac tgtcattcca tcggctatag tcgtgatccg
    11581 ggaagtagac agcattcaga ttgtgaaatt gtaaggtggg gttttgccgg ttggcccatc
    11641 cgcgatagcc atgatcgccg cacaggatga tgaccgcgcc ggggtcgttc cgacggatgc
    11701 tcgcgagcaa aggctccaga cgacggttag tgtagcggac ataaccaaga taagcggaag
    11761 aggggttggt ggatctctcc tcatatatct cgcggtcgct tttccggcgt cccagggagt
    11821 cgaacaaata agggaaatgc ggcatcatga aatgggcgta gatgaatttt ggccgaccgt
    11881 cgttgtggtc cgcctccgac gccacggcag taaggaactc ctcattcgag gcggcatatt
    11941 tcatgtaatc ctgaacgacg aaatgcctta tccccaggtc tctgatgacg agggaattcc
    12001 aatgtctttc cagccgctgt cccatcgttc ttgccgtgat aagccgcgat ttcaggggta
    12061 agaaggattg ccaggccggc gcgggatggc cggcgagatc aaagatggaa taattcttta
    12121 tcgcatagcc ttccgcggaa aagatacgga ccacgcggct atccctgatc agctggctgg
    12181 tgttggtgta atcctgcgac acgatatgca ggggttcctt tatcccttcg atataggcca
    12241 tattcagcac ggaagccatc gagaatggcg taagattata attggagcgg cttcggccgt
    12301 tgatatggaa cccctgtctg agcaggaaac tgtccaggtc gttgtcgaaa tgatattgct
    12361 ctttgagcgc aagactgccg gcgtattcat cgaaaaggag aaaataaacg ttaggcttgg
    12421 cgcatgtccc gcacgggggg gtgccttcat ctttcacgga caaaggatta acgggaggat
    12481 ggagggcctt tacgataagc ccgccggcat cggtcacgat atatatgatc aggagtacat
    12541 tcaggaacat gtacagccgc gagaacgtgc ctctaccctt tttcaccacc aggaatacgg
    12601 cgatcagcaa ccccagccca aacgacagga gaaacccatt cgtcgtaaag aaccggtgtg
    12661 ggaaatgccg ggtcatccat tggatgaacg cctcgtaaaa gaagtaaaaa gccatgatgg
    12721 cggcagtcag gat
//
LOCUS       DASYSV010000312         6740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1340480, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000312 DASYSV010000000
VERSION     DASYSV010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340480"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VGQ51_09320"
     CDS             complement(<1..395)
                     /locus_tag="VGQ51_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEV9036810.1"
                     /translation="MIAFLFMIVVGAGRPPVSSSMSADILRYVNEHRRSIGLKPLQAN
                     SYISSVALGHSRNIQSGRTPFGHDGFNQRIDLIKKRLGPLHVAAENVAEGPMTAREVV
                     DGWLKSPGHRRNIEGDFRLTGIGVAAARNG"
     gene            447..1130
                     /locus_tag="VGQ51_09325"
     CDS             447..1130
                     /locus_tag="VGQ51_09325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9036811.1"
                     /translation="MHPLDNPAWSALTTEQAKFARGAEYAKRYLPEILPFVGLASGAA
                     PEHADPYIAPGESFYIIGDLPPLPAGWKQEHELPCAQLLAPENLHCMPQPIEPILLLG
                     EPDKADMFGLIDSLQPGYYHPDTRRTGDYCGIRRDGRLVAMAGERMRMTGFSELSAIC
                     THPDFTGHGYAQQLIAWLCRRQVAAGITPFLHVSLANQRALRLYLYLGFRHRREITFR
                     RMRKSHPLR"
     gene            complement(1115..2125)
                     /gene="fbp"
                     /locus_tag="VGQ51_09330"
     CDS             complement(1115..2125)
                     /gene="fbp"
                     /locus_tag="VGQ51_09330"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEV9036812.1"
                     /translation="MIVNRKVLTLDEFTLQQLRNFPNATGELSNLLRDIGLAAKRINV
                     EVNKAGLVDILGDAGTVNVQGEDVKKLDVYANDQFMGVLRHGISCAGIGSEELDDSVV
                     FDDDISNNSKYVCLFDPLDGSGNIDVNVSIGTIFGVYRRISEKGKPCTKEDFLQPGRN
                     QVAAGYIVYGSSTMMVYATRRGVNGFTLDPSIGEFTLSHPNIQCPETGKIYSVNHGNF
                     FQYDGRVQAYIDACQKKTKANGGPYTQRYIGSMVSDVHRNLIKGGIFMYPATTSKPGG
                     KLRLLYECNPFAFITEVAGGKATDGTRRILDIQPTQLHQRSPFFVGSRLMMEELETYL
                     KG"
     gene            2167..3429
                     /locus_tag="VGQ51_09335"
     CDS             2167..3429
                     /locus_tag="VGQ51_09335"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV9036813.1"
                     /translation="MQVFKFGGASTSSIDRISKIPAILGPYRDQPLLIVISAMGKTTN
                     ALEKVAEAFYEGHREESLRLFGILKESHLTTAKYLLVQNYLAAEAQLRDFFTEIEWLL
                     HDKPVREYDYYYDQIVCIGELLSTSIISAYLAESGIGNQWIDVRDIFRTDANFRDATI
                     DWEYTGLKVKEEVLPLFGEEKIVVTQGFIGATSDNESTTLGREGSDYSAAVFAHLLNA
                     EGLTIWKDVEGVMNADPKQFPDAKILRELNYDEVIEMAYYGAQVIHPKTIKPLQNKDI
                     PLFVRCFLDAGLPGTIIHNETVRGLPPIIVLKNDQVLAHLHSRDFSFVGESGTARLYD
                     LFGEARIKPNLIQSGAVSLQVCLDDRPAKIEKLALAAAGLFDVQIERGLTLLTIRHYD
                     LEVLGRLTAGRKEVLRQQTPETVQILMR"
     gene            complement(3468..4673)
                     /locus_tag="VGQ51_09340"
     CDS             complement(3468..4673)
                     /locus_tag="VGQ51_09340"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV9036814.1"
                     /translation="MLLLQTAVVGFLSIGTVCAQNSAPSIRFIDYQRGVTPKVGEILH
                     RKEDTLLKQFREKNLVWPARYVYIRSFKYDSELEVWVKNGSQEKYKLFKTYKICALAG
                     TLGPKRMAGDYQTPEGFYYINEFNPKSEYHLSLGLNYPNASDRILSDSLQPGGDIYIH
                     GSCVTTGCIPITDNQIEELYTICAHAKDMGEDFIPVHIFPVNFNNPRSVGYLNKYLQT
                     FSEYTPFAKSMRSAFFYFEKYREVPFVMVNGKGEYVTEEAATSEAEVKIPDLEPVVTA
                     ATKKKPVHLRPERPNPIRDEDLAKSVSRLPVYPGGNEAFQTFLNQLGKDAVKDLDAGQ
                     TKAFIMVEYVIDSVGRPVYARVTRGGNEVVNEKIEDAFLAMPQWSPAAHGTAHVPIKL
                     KQTVMIGQD"
     gene            complement(4817..5911)
                     /gene="hppD"
                     /locus_tag="VGQ51_09345"
     CDS             complement(4817..5911)
                     /gene="hppD"
                     /locus_tag="VGQ51_09345"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEV9036815.1"
                     /translation="MSIDFLPLQGTDYVEFYVGNAKQAAHFYKSAFGFQSVAYSGPET
                     GDKDKVSYVVRQNKLTFVFTTALIPDHPIAGHTHMHGDGVKALALRVDDAASAWRETT
                     ARGARSYMEPTTLADKCGEVILSGIHTYGETVHLFVERKNYRGNFMPGFREWHSAFNP
                     PSTGLQYVDHCVGNVGWNQMDPWVRFYEDVMGFRNILSFDDNDISTEYSALMSKVMSN
                     GNGFVKFPINEPAEGKKKSQVEEYLEFYRGEGVQHVALATADIVTTVTQLRDRGVEFL
                     QVPGSYYDDLLQRVGSIDEDLQPLKQLGILVDRDDEGYLLQIFTKPVEDRPTVFFEVI
                     QRKGAKSFGKGNFKALFEAIEREQSARGNL"
     gene            6218..>6740
                     /locus_tag="VGQ51_09350"
     CDS             6218..>6740
                     /locus_tag="VGQ51_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV9036816.1"
                     /translation="MDPSSRQTIIMIKVGILGGGQLGRMLLQAAVNYPVETYVLENDE
                     HCPAAHLCHHFTKGDIRNFDDVYRFGKGLDALTIEIESVNEDALEKLEAEGVRVYPKP
                     SALRTIKNKITQKQFYSANSIPTAEYIVTENLDGVRSRIDFLPAVHKIGMGGYDGKGV
                     QILRSMEDVEKGFN"
BASE COUNT         1574 a         1937 c         1678 g         1551 t
ORIGIN      
        1 ccattgcgcg ccgccgccac cccgatgccc gtaagacgga aatcgccctc gatgttgcgc
       61 cgatgccccg ggcttttcag ccatccgtcc accacttccc ttgccgtcat cggaccctca
      121 gcgacatttt cggccgccac atgaagaggc ccgagacgtt tctttatgag atcgatacgc
      181 tggttaaagc catcatgccc gaagggcgtc cgaccgctct ggatgttccg gctatgcccc
      241 aatgcgacgg aggaaatata cgagttggcc tgcaacggct tcaacccgat agaccggcga
      301 tgttcattta cataccgcag gatgtcggca gacatggagg aggaaaccgg cggtctcccc
      361 gccccgacca cgatcataaa taagaatgca atcatgcgaa gtcctcatcc aataatgtac
      421 cgaaaattga taatttcacg ctaaatatgc atcctttaga caacccggca tggtccgccc
      481 tgacgaccga acaggcgaaa ttcgcacggg gcgccgaata cgcgaagcgc tacctgccgg
      541 aaatactgcc tttcgtgggg ttggcatcgg gagcggcccc ggaacacgca gatccttata
      601 tcgccccggg tgagtcgttt tatatcatcg gcgatctccc accccttccg gccggctgga
      661 aacaggaaca cgagctgccc tgcgcacaac tgctcgcacc ggaaaatctg cattgcatgc
      721 cccagcccat cgaacccatt ctcctgctgg gcgagccaga taaagccgat atgttcggac
      781 tgatcgatag tctccaaccc ggttactatc atcccgatac acggcggacg ggcgactatt
      841 gcggcatccg ccgggacggc cggttggtgg ccatggcggg tgaaaggatg cgaatgaccg
      901 gcttcagcga actgagcgct atctgtaccc acccggattt caccggacat ggctacgcgc
      961 aacagctgat cgcctggctt tgccgccggc aggtcgcggc aggcattaca ccctttctcc
     1021 atgtttcgct ggccaaccag cgcgcactac ggctttattt atatttgggt ttccgccacc
     1081 gccgcgagat cacgttcagg cgtatgcgta agtctcatcc cttgagataa gtctccaact
     1141 cttccatcat cagcctgctg ccgacaaaga atggcgagcg ctgatgaagc tgggtaggct
     1201 gtatatcgag gatgcgccgg gtgccgtcgg tggcctttcc tccagcgacc tcggtgataa
     1261 aggcaaaagg attgcattcg tacaataacc gcaatttccc gccgggtttc gacgtcgtcg
     1321 cgggatacat gaagatacca cccttgatga gattgcgatg gacatcggat accatcgatc
     1381 cgatataccg ctgcgtatac ggccctccgt tggccttcgt ctttttctga caggcgtcga
     1441 tatatgcctg cacccgaccg tcgtactgaa agaaattccc gtggttcacc gaatagatct
     1501 tgcctgtctc gggacattgg atattcggat ggctaagtgt gaactcacct atcgacgggt
     1561 cgagcgtaaa accgttcacg cccctcctcg tcgcatagac catcatcgtg gacgacccat
     1621 acacaatata gcccgccgcg acctggttcc gccccggttg cagaaagtct tcctttgtac
     1681 agggcttgcc tttttcactg atccgccggt aaactccaaa aatagtccct atgctcacat
     1741 tgacatcgat attcccgcta ccgtccagtg gatcgaaaag acagacatat ttgctgttat
     1801 tactgatgtc gtcgtcgaag accacactgt catccaattc ttcactgccg atgccggcac
     1861 agctgatccc atgacggaga acgcccatga attggtcgtt ggcataaacg tccagcttct
     1921 tgacatcctc cccctgcacg ttgacggtac cggcatcacc cagtatgtcc acgagaccag
     1981 ccttgttcac ctctacattg atccgtttgg ccgccaggcc gatgtcccgg agcagattgc
     2041 tcagttcgcc ggttgcgttg gggaaattgc gtaattgttg gagcgtgaat tcgtcgaggg
     2101 tgagaacttt tctattgacg atcatagtaa tgagcagttt atccgcaaaa ataggatatt
     2161 tgcgccatgc aagtttttaa gttcggaggt gcaagcacat ccagcataga tcgtatatcc
     2221 aaaatccccg ccatcctcgg tccctatcgc gaccagccct tgctgatcgt catctccgcc
     2281 atgggcaaaa cgaccaatgc gctcgaaaaa gtcgccgagg ccttctacga ggggcatcgc
     2341 gaagaatccc ttcggctttt cggtatcctt aaagagtcgc acctcaccac cgccaaatac
     2401 ctgctcgtcc aaaactatct cgcggccgaa gcccagcttc gcgatttctt caccgagata
     2461 gaatggctgc tgcacgacaa acccgttcgc gaatacgatt actattacga ccagatcgtt
     2521 tgcatcggcg aactgctgtc cacttccatt ataagcgcat atctcgccga atcgggcatc
     2581 ggtaatcaat ggatcgacgt ccgcgacatc ttccgcaccg atgccaattt ccgcgacgcc
     2641 accatcgact gggaatatac gggactaaaa gtgaaggaag aggtccttcc gctcttcggc
     2701 gaagagaaga tcgtcgtcac gcagggattc atcggcgcca ccagcgacaa tgaaagcacg
     2761 accctcggtc gcgaaggtag cgattactcg gcggcagtct tcgcccatct gctgaatgcc
     2821 gaaggcctga ccatctggaa ggacgtggag ggggtcatga atgccgatcc caaacaattc
     2881 ccggacgcga agatccttcg tgagctgaac tatgacgaag tgatcgaaat ggcgtattat
     2941 ggcgcacagg tcatccatcc caagacgatc aagccgctgc aaaacaagga tatcccgcta
     3001 ttcgtcaggt gcttcctcga tgccggtctc cccggcacga tcattcacaa cgagacggtc
     3061 agaggcctcc ctccgatcat cgtcctcaag aacgaccagg tgctggcaca tctgcactcc
     3121 agggattttt cctttgtcgg ggagagcggt accgcgagac tctatgatct tttcggcgag
     3181 gccaggatca aacccaatct catccaaagc ggagcggtaa gcctgcaggt ctgtctcgac
     3241 gaccggccgg cgaagatcga gaagctggca ctcgcggccg cgggcctttt cgatgtacag
     3301 atcgagcggg ggttgaccct gctgacgata agacactatg atctggaagt gctcggccgg
     3361 ctgaccgcag gccggaaaga agtgctccgg cagcaaactc cggaaactgt acaaatactg
     3421 atgcgttaat cctgaaggcc ctccgcgccg tcgccgaagg cagacagtta gtcctgccct
     3481 atcattacgg tttgcttcaa tttgataggc acatgagcag tcccatgcgc cgcaggactc
     3541 cattgcggca tggccagaaa cgcatcctcg atcttttcat tcacgacctc attgccgcca
     3601 cgggtaaccc gcgcatagac aggccttccc accgagtcga tcacatattc caccataata
     3661 aaagccttcg tctgtcccgc atcgaggtcc ttgaccgcgt ccttccccag ctggttgagg
     3721 aaagtctgga aggcctcatt ccccccggga tataccggca gcctggaaac ggacttcgcc
     3781 aggtcttcat cccggatagg attgggccgc tcaggccgca gatgaacggg ctttttcttc
     3841 gtcgcggccg tcaccacggg ttcgagatcg ggtatcttca cttccgcttc tgatgtagcg
     3901 gcctcttcgg tcacatattc ccctttcccg ttgaccatga caaatggcac ctccctgtat
     3961 ttctcgaaat agaagaacgc gctgcgcatg cttttggcga acggagtata ttccgagaag
     4021 gtctgaagat atttattcag ataaccgacg ctgcggggat tgttgaaatt gaccgggaag
     4081 atatgcaccg ggataaaatc ctcgcccatg tcctttgcat gtgcacagat ggtatacaac
     4141 tcctcgatct ggttgtcggt gatgggtatg caacccgtcg ttacacaact accgtggata
     4201 tagatgtcac ccccgggctg taacgagtcg ctgaggatac gatcggaagc attggggtaa
     4261 ttaagtccca gcgaaagatg gtattcactt ttgggattga actcattgat ataatagaac
     4321 ccttccggcg tctggtagtc ccccgccatc cttttcgggc ccagcgtacc cgccagcgca
     4381 catatcttgt aggtcttgaa aagtttgtat ttttcctgcg agccattctt gacccaaact
     4441 tccagctcgc tatcgtactt gaagctccgg atatagacgt accgggccgg ccagaccagg
     4501 ttcttttccc tgaactgttt taaaagcgta tcctccttcc gatggagtat ctctcctact
     4561 tttggggtta ccccgcgctg ataatcgatg aaacgaatgg atggtgctga attctgcgcg
     4621 caaacagtgc cgatggacag aaatccgacg acagcagttt gcagaagaag catggagtga
     4681 cgttttgcga gcatacgaga ggtacgaact ccgtcctgga atccttcccc aaagttgcaa
     4741 aaaacctgcc atattttata gctccaccct aaagatttcc cctccgcccg tcgatgccca
     4801 cagccgtcat caccccctaa agatttcccc tggccgattg ctcccgctct atcgcctcga
     4861 acaacgcttt gaaattgccc ttaccgaagc ttttcgcccc tttccgctgg atgacctcga
     4921 aaaagacggt gggcctgtcc tcgaccggct tggtaaaaat ttgtaataag tacccctcat
     4981 cgtcccggtc gaccagtatc cccagctgct tcaaaggttg cagatcctca tcgatgctgc
     5041 ccacacgctg cagcagatcg tcataatagc tgcccggcac ctgtaggaat tctacacccc
     5101 gatcgcgcaa ttgggtgacg gtcgtgacga tgtcggccgt agccagggca acatgctgta
     5161 caccctcacc cctgtagaat tcgaggtatt cctcgacctg cgatttcttc ttcccttcgg
     5221 ccggctcgtt gatggggaat ttaacaaatc cgttaccatt gctcatgacc ttgctcatca
     5281 gcgcggaata ctccgtggag atatcgttgt catcgaaaga caggatattg cggaaaccca
     5341 tcacgtcttc ataaaaacga acccagggat ccatttgatt ccagcctaca ttaccgacac
     5401 aatgatcgac atactgcagt cctgttgaag gcggattgaa ggccgaatgc cattcccgga
     5461 aacctggcat gaaatttccc cgataattct ttcgttcgac gaagagatgc acggtttctc
     5521 cataggtatg gataccactc aggatcactt cgccgcattt gtcggcaagc gtggtcggtt
     5581 ccatatagct cctggccccc cgggccgtcg tttcccgcca ggcgctcgcg gcatcgtcca
     5641 ccctcagcgc aagcgctttc acgccatcgc catgcatatg cgtatgcccg gctatcgggt
     5701 ggtcgggaat gagggcggtc gtaaagacga aggtcagctt gttctgacgc acgacatagc
     5761 tgaccttatc cttgtcccct gtttccggac cgctataagc caccgactga aagccgaaag
     5821 cggatttata gaaatgggcc gcctgtttgg cgttgccaac atagaattcg acataatccg
     5881 taccctgcaa tggcaggaaa tcaatagaca tataaatatt ttttaaattg gagggaggaa
     5941 taccgcagca cggggatcat tgacggacgc aggcttcaga ccgcacgcgc gttctcgtcc
     6001 cgagccgcag ataagggcgc aaacgcttgt cgtcgaaaat tttatgtgaa tagtcgggac
     6061 gcatgatctc tacattaaag gtagaaataa tttcgctggt tttcaaccga cgcggaatac
     6121 caccaaaaaa tcgtattttt tctattgcgg cgagtatttt tactctatcg tcccgggttt
     6181 tgagaattgg gaatcgagtt ttgtctatct tcgacccgtg gacccatcgt cccgacaaac
     6241 gatcatcatg ataaaagtag gcattttggg tggagggcaa ttaggaagga tgctgttgca
     6301 ggccgctgtc aattatcctg tcgagaccta tgtcctggaa aacgatgagc attgccccgc
     6361 ggcacatctt tgccatcatt ttacaaaggg agatatccgg aatttcgatg acgtctatcg
     6421 ctttggcaaa ggtctcgacg cactgaccat cgagatcgaa tccgtcaatg aagacgcgct
     6481 cgaaaaactg gaggccgaag gtgtccgggt ctatcccaaa ccatctgccc tcaggaccat
     6541 caagaacaag atcacccaaa aacaattcta ttccgcgaac agcattccga cggcggaata
     6601 tatcgtcacc gagaatctcg acggtgtgcg gagcaggatc gatttccttc ctgccgtcca
     6661 taagatcggg atgggcggat atgacggcaa aggcgttcag atcctgcgct cgatggaaga
     6721 tgtggagaaa ggttttaacg
//
LOCUS       DASYSV010000313         3558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_958223, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000313 DASYSV010000000
VERSION     DASYSV010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958223"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /locus_tag="VGQ51_09355"
     CDS             complement(<1..2177)
                     /locus_tag="VGQ51_09355"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEV9036817.1"
                     /translation="MPTTTPSAKGLQFSRRFTREDTNVFEQFGYDYRTSVIRNPSGEV
                     VFEMNNVEVPSGWSQIATDILAQKYFRKAGVPQPDGSLGRETSVKQVAHRMANCWRVW
                     GERYGYFDSPKDAQVFYEELVYSILDQACVPNSPQWFNTGLHESYGITGKPQGHYYVD
                     QADGQLKLSTSAYERPQPHACFILSVEDDLVNEGGLMDLWVREARIFKYGSGVGTNFS
                     HIRGEGEKLSGGGTSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVCLDLDHPEVMGFI
                     NWKVEEEKKVAALIAAGYPSDYEGEAYRTVSGQNSNNSIRIPNSFFHRLANDEDWELK
                     ARQDGRVMKRVPSREVWNQIAYAAWRCADPGTQYDTTINEWHTCPSGGPIRASNPCSE
                     YMFLDNTACNLASVNLRRFYDETTNTFDVEGFEYSVRLWTTVLEVSVLMAQFPSKEVA
                     QLSYDYRTLGLGYANLGSMLMIMGIPYDSEEARGIAGAITAMLTGVSYKTSAEMASVL
                     GPFPRYEANRESMLRVMRNHRLAAYDADEYEGLEIKPQGLKAKYCPDYLLKAATKAWD
                     EAVQLGEKYGYRNAQSTVIAPTGTIGLVMDCDTTGVEPDFALVKFKKLSGGGYFKIIN
                     QSVPTALKNLRYTDKQIDAIIKYAVGSGTFAGAPSVNHQSLSEKGFIAEEIKKLDAAV
                     GSAFEIGFVFNVHTLGEECLQRLGFEPAQYFNFEWSLLEALGFP"
     gene            complement(2400..2690)
                     /locus_tag="VGQ51_09360"
     CDS             complement(2400..2690)
                     /locus_tag="VGQ51_09360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9036818.1"
                     /translation="MDTIEVINNPEREQFQVNIDGETASLEYRFSDGVLVLMHTEVPE
                     KSGGKGIASALAAYAFSYAREKGLRVKVYCPFVQAWLKRHPEVADLLTPGRD"
     gene            2773..3534
                     /locus_tag="VGQ51_09365"
     CDS             2773..3534
                     /locus_tag="VGQ51_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HEV9036819.1"
                     /translation="MKKAIYESLLAKKREGQRSLAVLIDPDHVDAEKIEELTGLAVSA
                     GVDYLFVGGSLVISNHLDEVVQLVRSHCSIPVILFPGTPSQVSRFADGLLYLSLISGR
                     NPELLIGAHVISAPAVRTSGLEIISTGYMVIDGGAPTTVSYISNASPIPADKKEIALC
                     TAMAGEMLGMKLIYMDAGSGARTPIREEMIACVAENIEVPLVVGGGIRDPEKAYRNCK
                     AGADLIVVGNAIEKDASLIHEMAAAIHSVPLLHNQ"
BASE COUNT          802 a         1040 c          884 g          832 t
ORIGIN      
        1 gggaagccca gcgcttccag gaggctccat tcgaaattga aatattgtgc aggctcgaaa
       61 cccagccgct gcagacactc ttcgccgagc gtatgtacat tgaatacgaa accgatctcg
      121 aaggccgagc ctacggccgc atccagcttt ttgatctctt ctgcgatgaa ccctttttcg
      181 ctcaggctct ggtggttgac ggaaggcgca cctgcaaagg tccccgatcc gactgcatat
      241 ttgataatgg cgtcgatctg tttgtcggta taacggagat tcttcagcgc cgtgggtacc
      301 gactgattga tgatcttgaa atatcctccc cccgacagct tcttgaactt gacaagggcg
      361 aaatcgggtt ccacaccggt ggtatcgcaa tccatgacca gaccgatagt gcccgtgggc
      421 gcgataacgg tggattgcgc attgcgatag ccatattttt ctcccaattg cacggcttca
      481 tcccatgcct tggtggcggc ttttaataaa tagtcggggc aatatttggc cttcaatccc
      541 tgcggtttga tctccagccc ttcatattca tcggcgtcat aggcggccag ccggtgattc
      601 cgcattaccc gcagcatgct ctcgcggttg gcttcatatc tggggaaggg accgagtacg
      661 ctggccattt ctgcggatgt tttataggag acgcctgtga gcatggccgt gatggccccg
      721 gcgatgccgc gagcctcttc actgtcataa gggataccca ttatcatcag catagaacct
      781 aagttggcgt aacccagtcc aagtgtgcga tagtcgtagg acagctgcgc tacttccttc
      841 gaagggaatt gcgccatcag caccgatact tccagcaccg tagtccatag ccgcacggaa
      901 tattcgaaac cttcaacatc gaaagtattg gtcgtctcat cgtagaaccg gcggagattc
      961 accgatgcga ggttacaggc cgtattgtcg aggaacatgt actcactgca gggattcgaa
     1021 gcccggatgg ggccgccgga gggacaggta tgccattcgt tgatggtcgt atcgtattgc
     1081 gtgccggggt cggcgcaacg ccaggcggca taggctatct ggttccaaac ctcgcgggaa
     1141 gggacacgtt tcatcacacg gccgtcctga cgcgctttca gttcccagtc ttcgtcgttg
     1201 gcgagacgat ggaagaacga attcgggata cggatcgagt tgttcgaatt ctgaccggag
     1261 acggtccgat aggcctcgcc ttcataatcg ctgggatagc cggccgcgat cagcgcggcg
     1321 acctttttct cctcttcgac cttccagttg atgaacccca tgacttccgg atgatccagg
     1381 tccagacaca ccatcttggc agccctgcgg gtggtgcccc cggatttgat cgcgcctgcg
     1441 gcgcgatccc caatcttcag aaagctcatg agtccgcttg aagtaccccc gccgctcagc
     1501 ttctcgcctt ctccgcgaat atgggaaaaa ttcgttccga cacccgaacc atacttaaag
     1561 atccgcgcct cccgtaccca aagatccatc aggccaccct cattgacaag gtcgtcctca
     1621 acgctcaata taaaacaggc atgcggctga ggtctttcat atgccgaggt cgacaatttc
     1681 agctgaccgt ccgcctgatc tacatagtaa tgtccctgag gcttacccgt aatgccatag
     1741 ctctcatgaa gccccgtatt gaaccattgc ggcgaattgg gcacacaggc ctggtcgagg
     1801 atgctgtaaa ccagctcctc ataaaatacc tgggcatcct tcggactatc gaaatagcca
     1861 taccgctcgc cccatacgcg ccagcaattt gccatgcgat gcgccacctg tttgaccgag
     1921 gtctcacgac ccagcgaacc gtccggctgc ggaacgcccg ccttccggaa atatttctgc
     1981 gcaaggatat cggtagcgat ctgactccac ccggaaggga cctccacatt attcatttca
     2041 aatacgacct ccccgctggg attccggatc accgacgtcc ggtagtcgta cccaaactgt
     2101 tcaaacacat tggtgtcttc cctggtgaag cggcgggaga attgtaagcc cttcgcagat
     2161 ggcgtagttg tcggcataag tcagtccctg tttaaaactt gaagatttgt tttagatgat
     2221 agtttttgcg gtgggtatcg gtggttaacg aaagtatttt ttcaatttga tatttccgcg
     2281 gtcaacttac gcacacccgt ggaaaacgaa tgttaatttt ttcgcgttgc aggcggagcc
     2341 tgcattagcc gtttccccac atgcctcaga caatattttt atgcacatcg acgtccccat
     2401 cagtcccggc ccggcgtcag cagatccgcg acctccggat gcctcttcag ccaagcctgt
     2461 acaaagggac aatatacctt cacccgcaac cccttctccc tcgcatacga gaaagcatat
     2521 gccgccaaag ccgaagcgat tcccttccct cccgatttct ccggcacctc cgtatgcatc
     2581 aacaccagca ccccatccga aaatcgatat tccaaagacg cggtctctcc gtcgatattc
     2641 acctggaatt gctcacgctc cggattgtta atcacttcga ttgtatccat ataacctaat
     2701 ttgcttattt tcgtcgaatt agctccctaa agcggaaaac ttttcgcaat tttgtagcac
     2761 caacggcaat agatgaagaa agcaatttac gaatccttgt tggccaaaaa aagagaaggg
     2821 cagcgctcgc tggctgtatt gatcgatccg gaccatgtgg atgcagagaa aattgaagaa
     2881 ttgacaggtc tggccgtcag cgcaggagtg gactacctat ttgtcggagg tagcctggtc
     2941 atttccaacc acctcgacga ggtggtccag ctcgttcgta gccattgctc catccctgtc
     3001 atcctttttc ccggtacacc atcacaggtc tcccggtttg cggacggtct cctctatctt
     3061 tcgctgatat cgggccgcaa tcccgagctg ctgatcggcg cccatgtcat atcggccccg
     3121 gccgtccgca ccagcggcct ggagatcatt tccaccggct atatggtcat cgacggcgga
     3181 gcccccacta ccgtgtctta tatcagcaat gccagcccga tcccggccga taaaaaagag
     3241 atcgccctct gcactgccat ggcaggcgaa atgctgggta tgaaactgat ctatatggat
     3301 gccggcagcg gcgcccgcac ccccatccgc gaagaaatga tcgcatgtgt agccgaaaat
     3361 attgaggtcc cgctggtagt cggcggggga atacgcgacc ccgaaaaagc ctaccgcaat
     3421 tgcaaggcag gagccgacct catcgtcgta gggaacgcca tagaaaagga cgcctctctc
     3481 atccacgaaa tggctgcagc catccattca gttccgctcc ttcataatca atgagttgcg
     3541 ccgcgctacg cgcggcgc
//
LOCUS       DASYSV010000314        12097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1212948, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000314 DASYSV010000000
VERSION     DASYSV010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12097
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212948"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /gene="zwf"
                     /locus_tag="VGQ51_09370"
     CDS             complement(<1..1592)
                     /gene="zwf"
                     /locus_tag="VGQ51_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV9036820.1"
                     /translation="MAKEKKILPAAVSIFGAKGDLTRRKLIPSFYNLFIDNHLPANFH
                     LFCVDFLPVTQDAYRDYLRENVDQFSRSGKADQTKWQAFASRITYVQGDFTKPETYEL
                     LKGEIKKFETTEKVRGVRMFYFAVAPRFIEPIADALCNHKLCNRDTHDRIVVEKPFGT
                     DLASAKQLNRYLGKRFTEKQIYHIDHYLGKEMVQNILAFRFANYVFEPLWNNKYIDYV
                     EISVAEEVSVGKRGGYYDTSGALRDMIQSHLLQLLCIIAMECPDSYDAEAIRDVKVKV
                     LKKIRPFTSESVFKNVVRGQYTAGKVHGEQRKSYREEEQVAPDSTTDTYVAAKFLIDN
                     DRWKNVPFFLRTGKNMARQSSVIMIQFKDTPHKIFKDDITPNRLMISIQPELEIGLLF
                     EGKVPGLHMKLLPVEMDFMYQGNYSQNLPEAYEALLLDVLEGDATQFMRADQIEWAWK
                     VVMPILDAWRKNPKKGLQFYEAGTWGPSAANALLKGYSKEWFVLDHPAGGATTPAAFD
                     DVHAPSQDFNGGKNVRGTADKAT"
     gene            complement(1643..3619)
                     /locus_tag="VGQ51_09375"
     CDS             complement(1643..3619)
                     /locus_tag="VGQ51_09375"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV9036821.1"
                     /translation="MRNSLPFLALTVLLLAQQTSRAQQSTTPAATGSGNYDPHEAFAP
                     IFYPMYGDDIRSAAGAPGRQYWQNSADYKIDASLDDENKSLTGTVLITYKNNSPQELP
                     FVWLQMDQNIYSLQSRGVAITDLSGGRWANRDQFDGGYQLKSVGLVNAATGKTSPADY
                     LVSDTRMQIKLPAALKTGGVLKIRIAYSFKVPQYGTDRMGRLETKNGWIYEIAQWYPR
                     MCVFDNVMGWNTLPYLGQGEFYLEYGNIEYNVNVPSDMIVAGSGELTNPAEVLTPNQI
                     RRLAQARTSDKTVAIRGVDEVTDPASRPSKGRLTWRFKCTNTRDVAWAASKAFIWDAA
                     RINLPSGKKALAESVYPIESAGDSAWGRSTEYVKGCIEFYSKYLYEFTYPSATNVGGI
                     VGGMEYPGIVFCGSRSRMGNLWGVTRHEFGHNWFPMIVGSNERKYPWMDEGFNTYINT
                     VADSAFNNGEYLRPVRSRARMARAFFEATNPMMSIPDAMPAREWGTLSYNKPGTGLEL
                     LREEVLGKDRFDYAFRYYIHEWAFKHPTPYDFFHAMENASGETLDWFWRGWMFNNWKI
                     DQAVTGVEYLQGDPSKGATITIENKEKMPMPVTVEVKEENGTTNRVHLPVEIWMKGGV
                     WKFHYSSTSKIIQVTIDPDQRYPDSDPNNNTFKP"
     gene            complement(3680..4417)
                     /locus_tag="VGQ51_09380"
     CDS             complement(3680..4417)
                     /locus_tag="VGQ51_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9036822.1"
                     /translation="MEFLHVALPHIDFVQRKGSHLAIAGETGSGKSTLLRTIAGLATP
                     AAGTVFFEGVRVRGPMERLIPGEPGIAWLSQYFELWHHYTVEEVLTYNNDLSPEEADG
                     LYRLCHIDHLLRRRTDQLSGGERQRIALARLLVHPPRLLLLDEPFSNLDMIHKRLLKT
                     VIRDVTEKYGITCILVSHDPYDTLSWADELLILRNGRIHQQGSPREVYSRPVDEYAAG
                     LLGEYSLVNGEFLRPEAQTAFFFSAHP"
     gene            complement(4429..5055)
                     /locus_tag="VGQ51_09385"
     CDS             complement(4429..5055)
                     /locus_tag="VGQ51_09385"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HEV9036823.1"
                     /translation="MTIGLLAFLHLAYGQFEGILYYDCTIKNKTLTTLYESRTKILLE
                     AKTYPMKEGAADISKGKEQDALIFDFGTNKMTRLSSRHKQALTTAMGPVTADRAADPK
                     EGDLTIQDAGAEKVNGYDCHHFVVTIKNKKSDLWITKDLGTSSLCLVAQFDYYPAGSL
                     LYDKLKAVGGDGVVVRCEAGGVTVNLTNVQRKTVPPSFFEIPEGYSKS"
     gene            5269..6054
                     /locus_tag="VGQ51_09390"
     CDS             5269..6054
                     /locus_tag="VGQ51_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036824.1"
                     /translation="MNPDTNLSEEEAILNELVHTLLYQGYSLFPFLEKTPGNRPPTPF
                     GVLYPERYCPFNPHMHCRMQTALVVKGTPAMRLSVRVRFLQVEKPGEAVEREISPRET
                     TIEKLLQARAACYFISTPASPADLKGRVLMQAFPIEGTDTAFRIVVGVENGTIPEETA
                     PVTAEIVLKHAFVSTHTILRIRDGEFVSNQNPGPEWEEAVKDCINDRTFPVLIGEDNR
                     IMLSSPVILYDHPRLHGDTRSDIFDNLESEETETPGLSLSGEG"
     gene            6212..6472
                     /locus_tag="VGQ51_09395"
     CDS             6212..6472
                     /locus_tag="VGQ51_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036825.1"
                     /translation="MRKYVILTTFFSVFHLLHAVSQVKTDGSARPTSASPARVRGSIK
                     GIVADTAGSSPMADATVSISPERDTSDIQFAITDQHIRKRNG"
     gene            complement(6500..8458)
                     /locus_tag="VGQ51_09400"
     CDS             complement(6500..8458)
                     /locus_tag="VGQ51_09400"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEV9036826.1"
                     /translation="MKKWFVFISGLLLTAGVRAQQAAGTAASLVIPRNVLQPFEDSLS
                     YILTSRADWHPFSRTPEEWQRSLPDSVLRRIVHEGEAELGFEFKPLPATLLLEYSRTG
                     NRTHYENVSFGKRNALWRLILAESVEGQGRFTDKIADGIWSICEESYWGVPAHLGLQK
                     AGVGLPDVADPTTDLFTAETAAVLAWADYFNGAQLDKVSPLIRKRIYDEVSRRVFAPL
                     ATAKYGYLGGGRTDVKLSNWAPWVMSNYCTAALLLERDSARRLDEIRRAMHYTDLYID
                     GLGEDGGCEEGPTYWTAAGGCVFDVLCLLKDATKGRIDLYDRPIIRSMAQYIYTVHIA
                     GNYYVNIADAAPQLALDGPMVYRFGRAIDDAVMAHFGSWLAHTSPASPETYELFHRTR
                     ALYNLLALGDCDRYPASEPRVERAWLGEIQLMTVRGPNGLFLACHGGTNGESHNHNDV
                     GDFIIYKGGRPVIIDAGSGTYTARTFSGHRYDLWFNTSAYHNLPTINGYQQHDGLAYA
                     ATDVRYRFDDEGIVSLSMDIARAYPATAGVASWVRTVRMEKEKGISVTDRYKAAAPLR
                     SLTQSFMTVDTVDLGRPGVIVFDAGDDQRVMLNYDPAIWTASVGKVEFGIPEEQGVRE
                     HWQGKPIYRVLLTARKMPSASTIRWVFR"
     gene            8526..8894
                     /locus_tag="VGQ51_09405"
     CDS             8526..8894
                     /locus_tag="VGQ51_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9036827.1"
                     /translation="MADIQLGWFELCLNVADIAASVAFYHKLGFRQTEGSIGEQRVVM
                     QYGTCRVALYQGYLENNLLHFRGGDVVEIARRLEERGISSGKPPSEDEEGNQSYLIED
                     PDGNRVYFVHHPGEQPPPPL"
     gene            9006..11432
                     /locus_tag="VGQ51_09410"
     CDS             9006..11432
                     /locus_tag="VGQ51_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036828.1"
                     /translation="MFPNFLKVAWRNLWRGKAFSLINLVGLAIGMASALLILMWVHNE
                     FSFDSTYPASDRLYQGWNRGKYLHGIQCRNITPSPLGPALKTDYPEVERASRLSWDAS
                     QLFTIGDKKIDLTGAAADTDFLPMFGFPMLKGDPLTALKDPNNIVLTQKAARALFGDA
                     DAMGKTLRLDNKYDYTVTGVMKDLPNNTGFDFEYLLPWKYLVRTNQDDSDWEHNFIRN
                     YVLVRPHTDIARLNNKIRNIYQQHVKGVNVNEAFLYPVSRLHLYSNFENGVPAGGRIE
                     TVRVFLTIAVFILLIACINFMNMSTARSERRAKEVGIRKVSGALRSSLIGQFLGESIL
                     LSAVAGGIALLLVQVCLGPFNLLTQKQLVVEYVNPIFWLSFAGFIIFTGVLAGSYPAF
                     FLSAFRPVSVLKGYFKKAHQKELRRGLLRHAPSWFGTFVSLGSPRKALVVLQFTFAII
                     LIVSTLIIRRQVQYAQTRETGYNKDNIVWTYLSGNVAGKYDLIKSELLDKGIALAVTK
                     SSAPLSTSWQVNGAFWKGKNPNVHIDFNYFNEDGGIVKTAGLRLLQGRDIDTKQYPTD
                     STAVVLNEAAAKAMGFKDPIGQIINRGDWDVGWHVIGVVKDFVLESPFDAIKPMIILG
                     PKANWFNLMHIRLNGAKPTAQSLAEMGDIFRRYNPEYPFEYHFIDQEYARKFNDEQTT
                     ATLTALFAGLTILISCLGLFGLAAYMAEARIKEIGVRKVLGASVTGIVTLLSGDFIRL
                     VLIALLIAAPIAWWSMDKWLDNFDYHIAIPWWIFLAAGAIAVGIALGTVSFQAIRAAL
                     TNPARSLRSE"
     gene            complement(11463..>12097)
                     /locus_tag="VGQ51_09415"
     CDS             complement(11463..>12097)
                     /locus_tag="VGQ51_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9036829.1"
                     /translation="TAGGLVNGDVVTVSLTSNAVCASPDTVTSLPSIVAVLPRPGVTI
                     SGDTVGDPAATDHLVSIVVNGGVNPVYLWQDSTSAHSWQTIGGAAGGTVNYSPMVGGD
                     YVRCIVTGSDGCTAASNTLSLTVITIPNTVRYYPNPAGPTLYVENMDPMDPIETITVF
                     TAAGNRVISLGNLTSQVKIAIDVASLNPGIYYVSLTRQSGKTDRFQFMKR"
BASE COUNT         2724 a         3407 c         3198 g         2768 t
ORIGIN      
        1 gttgccttat cggctgtgcc ccggacgttt ttcccgccgt taaagtcctg cgagggcgca
       61 tgcacgtcat cgaaggctgc gggagtcgta gctccgcccg cagggtggtc gaggacgaac
      121 cattctttgg aatagccctt caacaatgca ttggcagccg atgggcccca ggttccggct
      181 tcatagaatt gcaacccttt ttttggattt ttcctccagg cgtccaatat cggcatgacg
      241 accttccagg cccattcgat ctggtcggcg cgcatgaact gcgtggcgtc cccttcgagg
      301 acatcgagaa gaagggcttc ataggcttcc ggcaaatttt gcgaatagtt cccctggtac
      361 atgaagtcca tttctaccgg aaggagcttc atgtgcagac cgggtacctt tccctcgaag
      421 agcaagccta tttccagttc gggctggatg ctgatcataa ggcggttggg tgtgatgtcg
      481 tccttgaata tcttgtgagg ggtatccttg aactggatca tgatgaccga ggattgcctg
      541 gccatattct tccctgtacg gagaaaaaat gggacattct tccagcggtc gttgtcgatg
      601 aggaatttgg ccgccacata ggtatcggtg gtggagtcgg gagccacctg ctcttcctcg
      661 cgataggatt tgcgttgttc tccatgcacc ttgccagcgg tgtactggcc gcgcacgaca
      721 ttcttaaata cagattcgga ggtgaagggg cgaatctttt tgagcacctt tactttcaca
      781 tcgcgaatgg cctcggcgtc atatgaatcg ggacactcca tggcaatgat gcaaagcagc
      841 tgcaataaat ggctctggat catgtcgcga agtgcgccgc tggtgtcata ataaccgccg
      901 cgtttaccga cgctgacctc ttcggcgacg ctgatctcga cataatcgat gtatttgttg
      961 ttccacaacg gctcgaatac atagttggca aagcggaagg ccaggatatt ctgaaccatt
     1021 tcttttccaa gatagtgatc gatatgatag atctgttttt ccgtgaaccg tttgcccagg
     1081 tagcggttga gttgtttggc tgaagccaga tcggtgccga agggtttttc gacgacgatc
     1141 cgatcgtgcg tgtcgcggtt gcagagtttg tgattgcaaa gcgcgtcggc gataggctcg
     1201 ataaaacgcg gtgccacggc aaaatagaac atacggacgc cgcgcacctt ttccgtggtc
     1261 tcgaattttt tgatctcgcc tttcagcagc tcataggtct ccggttttgt aaaatctccc
     1321 tgcacataag tgatacggga cgcgaacgcc tgccatttcg tttggtccgc ctttccggac
     1381 cggctgaact gatccacatt ctcgcgaaga tagtcgcggt aggcgtcttg cgtcaccggg
     1441 aggaaatcca cgcagaacag gtgaaaattg gcgggcagat gattatcgat aaagaggttg
     1501 taaaaagaag ggatcagctt gcgtctcgtc aggtctccct tggcgccgaa gatggaaacg
     1561 gccgcgggga ggattttttt ttcctttgcc atatccaaat atatgcaaaa aaaaagccgc
     1621 cccttcgggg cggcttcact tattaaggtt tgaacgtatt attattcgga tcactatccg
     1681 gatacctctg atccggatcg atcgtcacct gtatgatctt gctggtggag gaatagtgga
     1741 atttccacac tccgcctttc atccatatct ctaccgggag atgaacgcga ttagtcgtgc
     1801 cattttcctc tttcacttcc accgtgacgg gcatcggcat tttttcctta ttctcgattg
     1861 tgatggtagc gcccttcgac gggtcgccct gcagatattc gacccctgtt accgcctggt
     1921 cgatcttcca gttgttgaac atccagccgc gccagaacca gtccaatgtt tcacccgagg
     1981 cgttttccat cgcatggaag aaatcataag gagtgggatg tttgaacgcc cattcgtgga
     2041 tataataccg gaaggcatag tcgaaacggt ccttacccag cacctcttct ctcagcaatt
     2101 cgagacccgt tccgggttta ttataggata aggtgcccca ttcgcgcgca ggcattgcgt
     2161 cgggtatgga catcatggga ttggtggcct cgaagaaggc gcgcgccata cgggcccggc
     2221 tcctgacagg acgcaaatat tcgccattgt tgaacgcgct gtccgcaacc gtattgatat
     2281 acgtattgaa tccttcgtcc atccagggat attttcgttc gttgcttccg acgatcatcg
     2341 ggaaccagtt atggccgaat tcatgacggg ttactcccca gagatttccc atacggctgc
     2401 ggcttccgca gaagacgata ccgggatatt ccataccgcc tacgatcccg ccgacattgg
     2461 tcgcggaggg gtaagtgaat tcataaaggt atttggaata gaattcgata caacccttga
     2521 catattcggt cgaccggccc caggctgaat cgcctgcact ttctattgga tagacggatt
     2581 cggccagcgc ttttttgcca ctgggtaaat tgatccgggc tgcgtcccag ataaaagcct
     2641 tcgaggcggc ccaggccaca tcgcgggtgt tggtgcattt gaatctccag gtcaaccttc
     2701 ctttggaagg ccggctggca ggatcggtca cctcatccac gccacggatg gctacggtct
     2761 tatcgctggt ccgcgcctgt gccaaacggc gtatttggtt cggcgtcaga acttcggctg
     2821 gatttgtcaa ctcgccggat cccgccacga tcatgtcgga ggggacgttc acattatatt
     2881 caatattccc atactccagg tagaactcgc cctgaccgag ataagggagg gtattccatc
     2941 ccatgacatt atcaaaaacg cacatacggg gataccactg cgcgatctca tagatccagc
     3001 cattcttcgt ttcgagacgg cccatacggt cggtgccata ctgtgggacc ttaaaggagt
     3061 aggcgatcct gatcttgagg acgccgcccg tcttcagcgc cgcaggcaat ttgatctgca
     3121 tccgcgtgtc ggagacgaga tagtcggcag gcgaggtctt gcccgtcgcc gcattgacca
     3181 gccccaccga cttcaattga taaccgccgt cgaattggtc gcgattggcc caacggccgc
     3241 cggaaaggtc ggtgatcgcc accccacgcg actggagact ataaatattt tgatccatct
     3301 gaagccacac gaaaggcagc tcctgcgggc tattgttctt ataggtgatg agcactgtcc
     3361 cggtcaaaga tttgttctcg tcatcgaggc tcgcgtcaat cttgtaatcg gcgctgtttt
     3421 gccagtattg gcgaccgggg gcgcctgctg ccgaccggat atcgtcccca tacatgggat
     3481 agaagatcgg tgcaaatgct tcatggggat cgtaattgcc ggaaccggtg gcggcgggag
     3541 tagtcgactg ctgggcccgg ctcgtctgtt gggcaagcaa caggacggtc agggccagga
     3601 aagggagaga atttctcatg gtgaagattg tttttgtgac ggcgctaagg tagggtagcg
     3661 agccgcagtg cagacaattt taaggatgtg cggaaaaaaa gaaggctgtt tgggcttcgg
     3721 gccgcaaaaa ttcgccgttg acaaggctat attcccccaa taatccggct gcatattcat
     3781 ccacgggtct tgagtacacc tcccggggag aaccctgctg atgaatacgc ccgttccgca
     3841 agatcaatag ttcatccgcc caggacaggg tatcatatgg atcatgcgag acgaggatac
     3901 aggtgatccc atatttttcc gtgacgtccc ggatgacggt tttcagcaga cgcttatgga
     3961 tcatgtcgag attggagaac ggctcgtcca gcaatagtag ccggggcgga tggacgagca
     4021 gacgagccag cgctatcctt tgtcgttcgc cccccgatag ctggtcggtg cgacgccgga
     4081 gcagatgatc gatatggcac aaccggtaaa gcccgtcggc ctcttccggg ctgagatcat
     4141 tattgtaagt aagcacttct tccaccgtat agtgatgcca aagctcgaaa tattgcgaga
     4201 gccaggcgat gcctggctcc ccggggatca gccgttccat cggaccccgg accctgacac
     4261 cttcaaaaaa gacggttccg gctgcggggg tggccaggcc tgcgatggtc ctaagcaggg
     4321 tgctctttcc tgagcctgtc tcgcctgcga tggccagatg actgcccttg cgctgaacaa
     4381 aatcgatatg tggtaaggct acatgcaaaa actccatgcg gatgcccgtt agcttttgga
     4441 atacccttcc ggtatctcga aaaaggaagg aggtaccgtc tttcgctgta cattggtcag
     4501 gttgacggtg acaccacccg cctcacaccg gacaacgacc ccatctccac ccaccgcctt
     4561 cagcttgtca taaagcagcg agccggcggg ataatagtcg aattgggcaa ccaggcacag
     4621 ggatgaggtg cccagatcct tcgtgatcca aagatcgctc ttcttatttt tgatcgtgac
     4681 gacgaaatga tgacagtcgt atccgttgac cttttccgcc ccggcgtcct ggatggtcag
     4741 atcgccttcc ttcggatccg ccgcccggtc cgcggtcacc ggtcccatcg cggtcgtaag
     4801 ggcttgctta tgacgggagg aaagacgggt cattttattc gtgccgaaat cgaagatcag
     4861 ggcatcctgt tcctttcctt tggagatatc ggccgccccc tccttcatcg ggtatgtctt
     4921 tgcttccagg agtatcttgg tcctggattc atagagggtc gtgagcgttt tgttcttgat
     4981 cgtacaatcg taatagagta ttccttcgaa ttgtccatag gccagatgaa ggaaggccag
     5041 caatccgata gtcaaggtta gcttttttat ccgcatattt aggttggtct tcggtggtac
     5101 ggtcttttag caaatgttgt tccttctcaa tttttacggt cggctgggga aaaatcgcca
     5161 cagtagcggc tttccacata aggcggtgaa aaaatccagg tcgaaaaata cgtatatggt
     5221 tcgacttttg ctgcaattta ctgaacaatc ccataccagt taccatctat gaatccggat
     5281 accaatcttt cggaagaaga agcgatactg aatgagttgg tgcataccct tctttatcag
     5341 ggatacagct tatttccctt tctggaaaaa acgcctggca acagacctcc taccccgttt
     5401 ggagttcttt atcccgaacg gtattgcccg ttcaatccgc atatgcattg ccggatgcaa
     5461 accgcccttg tcgtgaaggg aacgccggct atgcggttat cggtacgcgt cagattcctt
     5521 caggtcgaaa aacccgggga agcggtcgaa cgtgaaatct cccccaggga gacgacgatc
     5581 gaaaaactcc tccaggcccg ggcggcctgt tattttatat ccactccggc ctcgccggcg
     5641 gacctcaaag gacgcgtcct gatgcaggcc tttcccatcg aagggacgga tactgctttc
     5701 cggatcgtgg tcggtgtaga aaacggtacc ataccggaag agactgcccc ggtcaccgcg
     5761 gaaatagtcc ttaaacatgc gttcgtttcc actcatacca tcctgcggat acgcgacgga
     5821 gaattcgtct ccaaccaaaa ccccggtccc gaatgggaag aggctgtcaa agattgtatc
     5881 aatgaccgga cctttcccgt gctcataggc gaagacaacc gcattatgct ttcctccccc
     5941 gtcatcctct acgaccatcc ccggttgcat ggtgacacca ggagtgatat cttcgataac
     6001 ctggaatccg aagaaaccga aacgccgggg ctttccctca gcggcgaagg ttgacccgtc
     6061 cgcgacccgt tgatccatcc gacgacccat cgatccatcc gcgaccgcca ttcaccggtg
     6121 gacaggtgca tttccccaat catacgctat tgtccaaaga cggcagaatt tgcccgtaat
     6181 tttagcagga ggttcccaca cccccttcgc tatgaggaaa tatgtcattc tgaccacgtt
     6241 tttcagcgtt tttcatctgc tgcacgccgt gtcccaggtg aagacggacg gttcggcgag
     6301 gcccacgtcg gcttccccgg cgagggtaag gggctcgata aagggtatcg tggcggacac
     6361 ggccggcagc tccccaatgg ctgatgccac cgtcagcatc agccccgaaa gggatacttc
     6421 ggatatacag ttcgccataa cggaccagca catccggaaa agaaatggtt gaaatacctc
     6481 tagcggtcag ttactccatc tatctgaaaa cccagcgtat cgtcgacgcc gaaggcattt
     6541 tgcgtgccgt caacagtacg cggtaaattg gcttgccttg ccaatgctcg cgaactccct
     6601 gttcctcggg tatcccgaat tctaccttcc cgacggaagc cgtccagatc gcggggtcat
     6661 aattcagcat cacccgctgg tcgtcccctg cgtcgaatac aatcaccccc ggccggccca
     6721 ggtcgacggt atccaccgtc ataaatgatt gcgtcaggct ccgcaagggc gcagcggcct
     6781 tgtaacggtc ggttacgctt attcccttct ctttctccat ccggaccgta cgcacccaac
     6841 tggcgacacc cgcggtcgcc ggataggccc tggcaatgtc catgctcagg gaaacgatgc
     6901 cctcatcatc aaaccggtat cttacatccg tcgccgcata ggcgagaccg tcatgttgtt
     6961 gatagccgtt gatcgtgggc agattatgat aagcggatgt gttgaaccag agatcatacc
     7021 gatggccgga gaatgtgcgc gccgtatacg tacccgagcc ggcatcgatg atcacgggcc
     7081 ggccgccctt ataaataatg aaatccccta cgtcattatg attatggctt tccccattgg
     7141 tgccgccatg gcaggcgagg aaaagcccgt tggggccacg taccgtcatg agctggatct
     7201 cacccaacca ggccctctca accctcggtt cggacgcagg ataacggtcg cagtcaccca
     7261 gggccagcag gttgtacaac gccctcgtac ggtgaaatag ctcataggtc tccggcgatg
     7321 cgggcgaggt gtgggccagc cagcttccga aatgcgccat cacggcatcg tcgatcgccc
     7381 tgccaaaacg atagaccatc gggccgtcga gggccagttg aggagcggca tcggcgatat
     7441 tgacataata gttgccggcg atatgcaccg tatagatgta ttgcgccatg ctcctgatga
     7501 tgggacggtc ataaaggtcg atacggcctt tcgtggcatc tttcagaaga cagagcacat
     7561 cgaacacaca acctccggcc gccgtccaat aggtagggcc ttcttcgcac ccgccgtcct
     7621 cccctagccc atcgatatac agatcggtat aatgcatggc ccgccggatc tcatccagcc
     7681 tgcgggcgct gtccctttcc aacagcaatg cggcggtgca gtagttggac atgacccagg
     7741 gggcccagtt gctcagctta acgtccgtac ggccgccgcc caaatatccg tatttcgccg
     7801 tcgccagagg tgcgaaaacc cgtctgctaa cctcgtcgta tatacgcttt cggatcagcg
     7861 gcgacacctt atccagctgt gccccgttga aatagtccgc ccacgcgaga acggcggccg
     7921 tctccgcggt aaagagatcg gtcgttgggt cggcgacatc cggcaagcct accccggcct
     7981 tttgcagccc gagatgcgca ggcacccccc aatagctctc ttcgcagatg ctccagatac
     8041 catcggcgat cttatcggta aaccggccct gcccttccac ggattctgcc aggatgagac
     8101 gccacaaagc attccgcttt ccaaacgaga cattttcata atgcgtccgg ttgcctgtcc
     8161 gtgaatattc cagcagaagc gtggccggaa gaggcttgaa ctcaaaaccc aattcagcct
     8221 cgccttcatg gacaattcgc ctcaggacag aatccggcag cgatctttgc cattcttccg
     8281 gcgtccggga aaaaggatgc cagtcggcgc ggctggtcag gatataggaa aggctgtcct
     8341 caaatggctg caggacgttg cgcggaatga ccagcgaagc cgcagtaccc gcagcctgct
     8401 gcgcgcggac tcccgcggtg agcaacaggc cggatataaa aacaaaccac tttttcatgg
     8461 acgtaaatat acatccatca ccggcgatca tgaacaagat atatctttga aaaaaaataa
     8521 gcttcatggc agatatccaa ctgggttggt tcgagctgtg tctgaatgtg gccgacatcg
     8581 ctgcctccgt cgccttctat cacaagctgg gtttccggca gacggaagga agcatcgggg
     8641 agcaacgtgt cgtcatgcaa tatggcactt gtcgcgtcgc gctctatcag ggttatctcg
     8701 aaaacaacct gttgcatttt cgcggcggcg atgtggtgga gatcgcacgg cggctcgaag
     8761 aacgcggtat ttcctccggg aaacccccga gcgaagacga agaaggcaac caatcctacc
     8821 tgatcgaaga ccccgatggg aatagggtgt atttcgtgca tcatccggga gagcagcccc
     8881 cgcccccact gtaacaaaac cgtacaccca ctgtatcatc cccgaaccgc gccacccgac
     8941 caaccatcac atccacctga taaccaatat gcgcaacagc ggcacgcaat tcgccgcccc
     9001 cctgtatgtt tcccaatttc ctgaaagtag cgtggcgcaa tctatggcgc ggcaaagcct
     9061 tctcgctgat caacctcgtc ggtctggcga tcggtatggc cagcgccctg ctgatcctaa
     9121 tgtgggtaca caacgaattc agctttgaca gcacctaccc ggccagtgac cggctctatc
     9181 agggatggaa ccgcgggaag tacctgcatg gcatccagtg ccggaatatt acgccctcgc
     9241 cgctggggcc ggcgctaaag acggattatc cggaagtcga aagggccagc cggctgagtt
     9301 gggacgcaag ccagcttttc accattggcg ataaaaagat agaccttaca ggtgcagcgg
     9361 cagatacgga tttcctgccg atgttcggtt tcccgatgct gaaaggcgac ccgctcacgg
     9421 cattgaagga cccgaataat atcgtactca cccaaaaggc ggccagagcc ctatttggag
     9481 atgccgatgc catgggcaag accctgcgcc tcgacaataa gtatgattat accgtcacgg
     9541 gagtgatgaa ggacctcccc aacaataccg ggttcgattt cgaataccta ctgccctgga
     9601 aatatctcgt gcggacaaac caggacgact ccgactggga gcataacttt atccgtaact
     9661 acgtcctggt caggccacat accgatattg cccggctgaa caacaagatc cggaatatct
     9721 atcagcagca tgtgaaaggc gtcaatgtaa atgaggcttt cctttatccg gtgagcaggc
     9781 tgcatctcta ttcgaatttc gagaatggcg ttcctgcggg aggcaggata gagacggtaa
     9841 gggtattcct gaccatcgcc gtctttattc tcctcattgc ctgtatcaat tttatgaata
     9901 tgagcacggc gcgcagtgaa cgtcgcgcga aggaagtagg catccggaag gtatcgggcg
     9961 ccctccggag ttcactcatc ggacagttcc tgggcgagag catcctgctg tccgccgtcg
    10021 ccgggggtat cgcgcttcta ttggttcagg tgtgcctggg gcctttcaac ctgctgaccc
    10081 aaaaacagct ggtggtcgaa tatgttaacc ccatcttttg gctgtccttc gcaggtttca
    10141 tcatcttcac cggagtcctc gccggcagtt accccgcctt tttcctttcc gccttccggc
    10201 cggtgtccgt gctgaaagga tatttcaaaa aggcgcatca aaaggaactt cgtcgggggc
    10261 tgcttcgaca cgccccctcc tggttcggaa ccttcgtttc actcggttcc ccgcgcaagg
    10321 cactggtggt cctccagttc accttcgcca tcatcctcat cgtctcgacc ctcatcattc
    10381 gcaggcaggt gcaatatgcc caaaccaggg agacaggcta taacaaggac aatatcgtat
    10441 ggacctatct gtcaggcaac gtcgcgggca aatacgatct tatcaaaagc gagctcctcg
    10501 ataagggcat cgcgcttgcc gtcacaaaat ccagtgcacc cctatcgacc agttggcagg
    10561 tcaacggcgc cttctggaag ggaaaaaacc ccaacgttca tatcgatttc aattatttca
    10621 atgaggacgg cggtatcgtt aaaacggccg gtctccggct cctgcaaggg agagacatcg
    10681 acaccaaaca atatccgacc gattcaacgg ccgttgtact taacgaggcg gcggcaaaag
    10741 ccatgggatt caaagaccct atcggccaga tcatcaatcg tggagattgg gatgtcgggt
    10801 ggcacgtcat tggcgttgtc aaagatttcg tcctcgagtc cccgttcgat gcgatcaaac
    10861 ccatgatcat tctaggtccg aaagccaatt ggttcaacct tatgcatatc cggctcaacg
    10921 gtgccaaacc cactgcacag agcctggcgg aaatggggga tatcttccga cgctacaacc
    10981 ccgaatatcc tttcgaatac catttcatcg accaggaata tgcgcgcaaa ttcaacgacg
    11041 agcagacgac ggccacgctt acggctctgt tcgccgggtt gaccatcctc atctcctgtc
    11101 tgggattatt cggactcgcc gcctatatgg cggaggcccg gatcaaagag atcggcgtcc
    11161 ggaaggtgct tggcgcctcg gtcacaggca tcgtgacgct cctttccggg gattttataa
    11221 ggctagtact gatcgctctg ctcatagcag cccccattgc gtggtggtcg atggataaat
    11281 ggctcgacaa tttcgactat cacatcgcca tcccgtggtg gatctttctt gcggcaggtg
    11341 cgattgcagt aggcatcgcg ctgggtacgg tgagttttca agccatccgc gcggccttga
    11401 ccaatccggc aagatcgctc cgcagcgaat gagatcacga gcgactacca cccgccatcc
    11461 gatcaccgtt tcataaactg gaaccgatcc gtcttacccg actgtctcgt caggctgaca
    11521 taatatatcc cgggattcag ggaagccaca tcgatggcga tcttcacctg gctcgtgaga
    11581 ttgcccagcg aaatcaccct gttccccgca gcggtgaaaa cggtgatcgt ttcgatggga
    11641 tccatgggat ccatattctc aacatagagg gtgggaccgg cagggttcgg ataataccgc
    11701 accgtattgg gaatcgtaat gaccgtgagc gacagcgtat tgctcgcagc ggtacaaccg
    11761 tcactgcccg tgacgataca acggacatag tctccgccca ccatcggcga atagttcacc
    11821 gtaccgccag cggcgccgcc gatcgtctgc caggaatgag cgctcgtact gtcctgccaa
    11881 agatagacag gatttactcc gccgttcacc actatcgaaa ccaggtggtc agtggcagcg
    11941 ggatcaccca ccgtatcacc cgagatggtc acacccggtc gcggcaacac agcaacgata
    12001 gaaggcaggc tggtcaccgt atccgggctg gcgcagaccg cattgctcgt cagcgatacg
    12061 gtgacgacgt ccccattcac caatccgccg gcagtat
//
LOCUS       DASYSV010000315         5106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_317423, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000315 DASYSV010000000
VERSION     DASYSV010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317423"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VGQ51_09420"
     CDS             <1..321
                     /locus_tag="VGQ51_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV9036830.1"
                     /translation="NAALRRLQEEMGFSTALEKSFDFIYRTEFGNGLTEHEFDHVFVG
                     FFDGAINPDAAEVSDYCFRSPDEICSDLASIPDRFTVWFHLAFPLIAAARTSAGQPIG
                     PLRP"
     gene            complement(350..859)
                     /locus_tag="VGQ51_09425"
     CDS             complement(350..859)
                     /locus_tag="VGQ51_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036831.1"
                     /translation="MKRILSVVVLLSMLTPATMAQLKAKAHCPDAIVVDVLNGTVNTT
                     VKPNTTQNEIKEFFPCFTSSEDEGSGAKCGGGVFFKDKDLYFYTLRKYVEVGPKFTGR
                     TSIKILGTKRGSLFNQLGNPKVKDDLWEAFEMQYGTLVLHFDVAGPNGKVKFFQFSTL
                     NSESLNLCE"
     gene            complement(926..3751)
                     /gene="carB"
                     /locus_tag="VGQ51_09430"
     CDS             complement(926..3751)
                     /gene="carB"
                     /locus_tag="VGQ51_09430"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV9036832.1"
                     /translation="MPKDSSIRSVLIVGSGPIIIGQACEFDYSGTQAARSLREEGVEV
                     ILINSNPATIMTDPMMADKVYLLPLTVESIEQILEENKIDAVLPTMGGQTALNLAKEA
                     EDLGVWERFGVKLIGVDIKAIDKAEDREKFRQWMIQLGVPVATAKTANSFLEGKEFAQ
                     EIGFPLVIRPSFTLGGTGGGFVHDKEDLDEALNRGLQASPIHEVLVEKAVLGWKEFEL
                     ELLRDANNNVVIICTVENFDPMGVHTGDSITVAPAMTLSDTAFQLMRNTAIMMMRDLG
                     NFAGGCNVQFALNPKTEEIIAIEINPRVSRSSALASKATGYPIAKIAAKLAIGYSLDE
                     LKNQITGTTSAYFEPVQDYVIVKIPRWNFDKFKGANDTLGLQMKSVGEVMAIGRSFTE
                     AVQKACQSLENNAVGLGYYGKSLMHAEELLEYIKTPKWDRIFRIKDALMAGISVGTIS
                     KATNGIDRWFLYEIQKICNMEKELAKHSFENLPLPLLQDAKRLGFSDAQIAKILGTVT
                     EEAVYEKRKSAGITRVYKMVDTCSAEFEAKTPYFYSTFEGGHSNESKVSDKPKIIVLG
                     SGPNRIGQGIEFDYCCVHGLLAIKECGYEAIMVNCNPETVSTDFDIADKLYFEPVYWE
                     HLWEIIEHEKPAGVIVQLGGQTALKLAEKLDAKGIPIIGTSFDSMDIAEDRGRFSDML
                     KELEIPYPDYGTAFDVDEAVQVANRVGYPVLVRPSYVLGGQRMRIVINDDEVEKAVVS
                     LLKHIPGNKILIDHFLDRCQEAEVDAIFDGEDFHVMGVMEHIEPAGIHSGDSHAVLPA
                     FNLSPMEVTTMEYYSEKIARALNIKGLINIQFAIKEGRVYVIEANPRASRTTPFIAKA
                     YQIPYLNVATKIMIGKHKLKDFSFEKKLEGFAIKEPVFSFNKFPGVKIELGPEMKSTG
                     EAIRFIPNLRDPYFRKLYKDRSMYLSK"
     gene            complement(3975..4187)
                     /locus_tag="VGQ51_09435"
     CDS             complement(3975..4187)
                     /locus_tag="VGQ51_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036833.1"
                     /translation="MKGVKNRMVVFMAVAVGGMIGVVKGEEAKQKWVFRVRSKYENRK
                     LIAQRKSIARKGGVLLDDIELASYHN"
     gene            4520..>5106
                     /locus_tag="VGQ51_09440"
     CDS             4520..>5106
                     /locus_tag="VGQ51_09440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV9036834.1"
                     /translation="MKYLFAAVLLSFSLQGFPQFFPPTGYPRNYFRDPLAIPISLAAN
                     FGELRPEHYHMGLDIRTQRRVNLPVYAAADGYIARVSVAPEGFGQAIYINHPNGYTTV
                     YGHLNRFFPALEAYVHREQYRRQSWQVNLDLPPSLFQVKKGDLIARSGSTGGSQGPHL
                     HFEIRRTKEDINLNPLLFGLPVEDHVPPHIMRLAW"
BASE COUNT         1105 a         1507 c         1233 g         1261 t
ORIGIN      
        1 aatgcggcct tacgcaggct tcaggaagag atggggttca gcaccgcgct ggaaaaaagt
       61 ttcgatttta tttacaggac agaattcggc aacgggctca ccgaacatga attcgaccat
      121 gtctttgtag gattttttga cggagcgatc aatcccgatg ccgccgaggt ctcggattac
      181 tgtttccgca gtcctgatga aatttgctcc gatcttgctt ccatccccga ccggttcact
      241 gtctggttcc acctggcgtt tcccctcata gccgcggccc gcacatccgc cggtcaaccc
      301 atcgggccac tccgcccgta accccggcca cgacagcgga acgccgcccc tactcgcaca
      361 gattcagcga ctccgaattc aaggtgctga actgaaaaaa tttgaccttc ccattcggcc
      421 ccgccacgtc gaaatgcaag accagcgttc catactgcat ctcgaaagcc tcccacaaat
      481 cgtcctttac cttgggattt cccaattgat tgaaaaggct gcctcttttg gtgcccagga
      541 tcttaatgct cgtccttccc gtaaatttgg gacccacctc cacatatttc cgcaaagtat
      601 aaaaatagag atccttatcc ttgaaaaaaa cgccgccgcc gcatttggcg cctgagcctt
      661 cgtcttcgct gctggtgaaa cacgggaaga attccttgat ctcgttctgg gtggtattgg
      721 gcttgacagt agtgttgacg gtgccattca gcacatcgac aacgatcgca tcgggacaat
      781 gggccttggc cttcagctgc gccatggtcg ccggagtcag catcgatagt aatacgacga
      841 cagaaaggat tcgtttcata cgtagagtgg tttttaggag tcttacgtac tacaaatatc
      901 ctgcctatca ggccgaacct cctgtttact tacttaggta catgctcctg tccttgtaga
      961 gcttgcggaa atacggatcg cgcaggttgg ggatgaaacg gatcgcttct cccgtggact
     1021 tcatctcggg cccaagctct atcttgacgc cggggaactt gttgaagctg aagaccggtt
     1081 ccttgatcgc aaacccttcc agcttctttt cgaagctgaa atccttcagt ttgtgtttgc
     1141 cgatcatgat cttcgtggcg acgttgagat agggtatctg gtaggcctta gcgataaagg
     1201 gcgtcgtccg cgaagccctg gggttggctt cgatgacata gaccctgccc tctttgatgg
     1261 caaattgtat attgatcagc cccttgatgt tcagcgcgcg ggcaattttt tcggaatagt
     1321 attccatggt ggtcacttcc atgggcgaca ggttgaaggc cggcagtacg gcatggctgt
     1381 ccccgctatg gatacccgcg ggttctatat gctccatgac acccatgaca tggaaatcct
     1441 ccccgtcgaa gatagcgtcg acctcggctt cctggcaacg gtcgaggaag tggtcgatca
     1501 ggatcttatt accggggata tgtttaagca gactgaccac ggccttttcg acctcgtcgt
     1561 cattgatgac gatacgcata cgctgaccgc cgagcacata gctgggtcga accagcaccg
     1621 ggtaaccaac cctgttggct acctgtaccg cttcatcgac atcgaatgcc gtcccatagt
     1681 cgggataggg gatctccagc tctttaagca tatcgctgaa ccggccgcgg tcttcggcga
     1741 tgtccatgct gtcgaatgag gttccgatga tcgggatccc tttcgcatcc agcttctcgg
     1801 cgagcttcag ggccgtctgt ccacccaatt gcacgatcac gcctgccggt ttctcatgct
     1861 cgatgatctc ccataaatgt tcccagtaga cgggttcgaa atacagcttg tccgcgatgt
     1921 cgaaatcggt ggaaaccgtt tcgggattac agttgaccat gatcgcctca taaccgcatt
     1981 ccttgatggc cagcaaaccg tgcacacagc agtagtcgaa ttcgatgccc tggccgatcc
     2041 ggttgggacc cgaaccaagc acgatgatct tgggcttatc gctgaccttg ctctcgttgg
     2101 aatgtcctcc ttcgaaagtc gaataaaaat aaggtgtttt cgcctcgaac tccgccgagc
     2161 aggtatccac cattttatat acacgggtga tgccggcgct ctttcttttt tcgtataccg
     2221 cctcttcggt gacggtacca aggatcttgg cgatctgcgc atcactgaat ccgagcctct
     2281 tggcgtcctg cagcaagggc aacggcaggt tttcgaagct atgcttagcc agctcctttt
     2341 ccatattgca gatcttctgg atttcgtaca ggaaccagcg gtcgattcca ttggtagcct
     2401 tcgatatcgt gcccacggag ataccggcca tcaaagcgtc cttgatcctg aagatccgat
     2461 cccacttagg agtcttgata tattccagca gctcttcggc gtgcatgaga ctctttccgt
     2521 aataacccag acctaccgca ttattctcca ggctctgaca ggccttctgt acggcctcag
     2581 taaaacttct tccaatggcc attacctcgc cgacgctttt catctgcagg ccaagcgtat
     2641 cgttggcgcc cttgaactta tcgaaattcc agcgtggtat cttgacgata acatagtcct
     2701 gtacgggctc gaaataagcg gaggtagtgc cggtgatctg gttcttcagc tcatccagcg
     2761 aatacccgat ggccagtttg gcggcgatct tggcgatagg ataacccgtc gccttcgacg
     2821 cgagggccga gctccggctg acacgggggt tgatctcgat ggcgatgatc tcttccgtct
     2881 tcggattcag ggcgaactgt acgttacaac caccggcgaa attacccaga tccctcatca
     2941 tcatgatggc ggtattgcgc atcagctgaa aagcggtgtc gctgagcgtc attgcaggcg
     3001 ctacggtgat cgagtctccc gtatggacgc ccatcggatc gaaattttcg accgtgcaga
     3061 tgatgacgac gttattgttc gcatcgcgca gcaattccag ctcgaactcc ttccagccca
     3121 gcactgcctt ctccaccagc acttcatgga tcggggaggc ctgcagaccc ctgttgagcg
     3181 cctcatccag gtcttcctta tcatgaacga acccgccgcc cgtgccgccc agggtaaagg
     3241 agggccggat caccagcggg aagccgatct cctgcgcaaa ctcttttcct tctaagaagg
     3301 agttggccgt tttggctgtg gcgaccggta cccccagctg tatcatccac tgccggaatt
     3361 tctcccggtc ctcagccttg tcgatcgctt tgatgtcgac gccgatcagc ttcacgccaa
     3421 accgttccca gacgcccagg tcttcggcct ctttggccag gttcagcgcc gtctggccgc
     3481 ccatggtcgg caatacggcg tcgatcttat tttcttccag tatttgttca atgctttcca
     3541 cggtgagagg cagcagatag accttatccg ccatcatcgg gtccgtcatg atagtggccg
     3601 gattgctgtt gatcaggatg acctccaccc cttcttcccg caggctgcgg gccgcctggg
     3661 tgcccgaata atcgaattca caggcttgac cgataatgat gggacctgaa ccgacgatca
     3721 ggacgctccg gatggaagaa tctttgggca ttgcggtaaa aaataaaatt gtgcaaaggt
     3781 aaggccgcgg gagccccgca ccaaaagaaa tatgtcccgg cgccggccgc tgcccccgcg
     3841 ccgtcacctc ccatcgaccg ccgccgtcac ctcccatcga ccgccaccac ctcccgtcgg
     3901 ccgccgccac cgggaatgaa aaagccccgg cagaagccgg ggctcggtat aactaacatt
     3961 tacgggaaat ataatcaatt atggtacgac gctaactcga tatcgtccag aagcacaccg
     4021 cctttgcggg cgatggactt ccgctgcgct atcagcttcc ggttctcata cttgctgcgt
     4081 acccggaata cccatttctg tttagcctct tcgcctttaa caacgccgat catgcccccc
     4141 acggccaccg ccatgaagac aaccattcta tttttaactc ctttcataac tgctcaattt
     4201 atgttgaacc cttctacttt aaagacaatt atcgtaagcc aaaagctgta ccaaattcta
     4261 aaaaaaacct acccggttcg tagtattttt ttaacggtcg gacccgcggt tgaactgcag
     4321 cgtcttatta acaaccactt caccttttgg ttggcgccga agccggatta tatacgcaaa
     4381 aattacttcg tcggatgcct caccccatat ctaagctgct tacccggtct aacccgtatc
     4441 actgtgggaa aatcaggctc acaatgggaa tagttgccgt ttgagtacct ttggttttta
     4501 atagcatctt aatctcgaca tgaaatacct cttcgcggcc gttctcctat ctttttcatt
     4561 gcaaggcttc ccgcaattct tcccgccaac cggatatccg aggaattatt tccgcgatcc
     4621 gctggccatt cccatcagtc tcgccgcaaa tttcggggag ctccgacccg aacactacca
     4681 catgggcctg gatatccgga cccaacgccg ggtgaacctg cccgtttatg cggcagccga
     4741 cggctatatc gcccgtgtgt ccgtcgcacc cgaaggtttt ggccaggcca tctatatcaa
     4801 tcatcccaat ggctatacga ccgtatacgg tcatctcaac cgatttttcc cggcactcga
     4861 ggcttatgtc caccgggagc aataccgcag acagtcctgg caggtaaatc tcgatcttcc
     4921 gccctccctt ttccaggtaa aaaagggcga tctcatcgca cgcagcggca gcactggcgg
     4981 ctcacaggga ccccatcttc atttcgagat ccgccgcact aaagaagaca tcaatctcaa
     5041 cccgctgctc ttcggtctgc cggtagaaga tcatgtcccg ccgcatatca tgcgtctggc
     5101 ctggta
//
LOCUS       DASYSV010000316         7460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2237156, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000316 DASYSV010000000
VERSION     DASYSV010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237156"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VGQ51_09445"
     CDS             <1..205
                     /locus_tag="VGQ51_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036835.1"
                     /translation="ASGNGTAILTLVDLYGRVIRRQQQQVTQGYNTVTMFGLSGLPVA
                     TYALQIQYNNQLVSEKMVKVNVQ"
     gene            complement(310..1011)
                     /locus_tag="VGQ51_09450"
     CDS             complement(310..1011)
                     /locus_tag="VGQ51_09450"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEV9036836.1"
                     /translation="MKARTIFLLVLLIIVADQSLKIWVKTHMPLSHPVDTYMEKISPY
                     DNGIRLAGDKAQIYFVENEGMAWGWKFGGSWGKMALTLFRLVAVVFGVYYIQTIIKKK
                     YHRGFIICAGLIFAGALGNLLDSMFYGLIFERSTYEHVAKIFPPHGYTTFLHGRVVDM
                     LYFPIIKTHYPGWFPFVGGQEFEFFSPVFNLADASISVGVIAILIFQKKFFHKRPGVE
                     NHPVEGQAKVDAVQV"
     gene            complement(1152..3644)
                     /locus_tag="VGQ51_09455"
     CDS             complement(1152..3644)
                     /locus_tag="VGQ51_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HEV9036837.1"
                     /translation="MEYSITYVNEVIGGRWIRFYADDAVSQLLLDSRRLIFPVSTLFF
                     ALKGARRDGGRYVEELYRRGVRNFVVGEGWEGSELPGANIIGVGDALVALQTLVAAHR
                     KHFALPVIGITGSNGKTIVKEWLNTLLEEDCQIARSPKSYNSQTGVALSVAQLGPAHQ
                     LAIFEAGISRRGEMSRLEPMVRPTIGVFTNIGEAHSEGFGSLAEKAAEKLKLFVRAEV
                     LVYCADQEETVKAVERWEGKARRWAWGRGEGNAVRIGPVEKGDGWAKIGVGSGGAGGE
                     GNAVFAVELPFSDHASVENALHCVAVLLFLRVPEEKIRERMRLLAPIAMRLELKNGIN
                     HCSIINDSYSADLSSLSIALDFLSQQQQHPRRTVILSDLLESGREEGELYALIARALG
                     QKAVDRLIGIGTHIGAHTSEFETWFPGERIFYPTVEAFSDDLSRLHFRDETILIKGAR
                     VFEFEKIDRLLTEQIHQTVMEVDLGAMAANLRAYRQVLDPATRIMAMVKAYGYGSGSA
                     EIAGLLQFHGIDYLGVAYADEGVVLRKAGIRVPIMIMNTETSSFDSLVEYNLEPVIYS
                     PALLQDFDRWLKKEGIPAFPVHLELETGMNRLGFPPEEMDVLAAALGTAAFRVMSVFS
                     HFAASEDAEQDGFTRGQGKLYHEMADRIGKALGYSFLRHMDNSAGIVRHPGLQMDMVR
                     LGIGLYGIDSAGSGRLQLEEVSTLKTTIAQIKRLHTGDTVGYNRLGQATEGTIIATVR
                     IGYADGYVRALGNGVGKMWVRGHLAPTIGAVSMDMTMIDITGIPGVSEGDEVVVFGNE
                     LSVSQLAKWAGTIPYEIMTGVSQRVKRIYYEE"
     gene            complement(3725..4672)
                     /locus_tag="VGQ51_09460"
     CDS             complement(3725..4672)
                     /locus_tag="VGQ51_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEV9036838.1"
                     /translation="MSPTDITYLTFGILLFLAILFDLGLLSKKNAPVTIRKALFQTVF
                     WVVLAMVFFGFLWYEDGQKIALEYVSAYLMEWSLSIDNIFVFILIFSFFRVKPQYYAR
                     VLLIGILLAIFFRIIFITAGIALVTRFHWLLYVFGAALIYTGITMFTADREAGMDVEE
                     NKVYKFMRRLLPLTGSDGDGRMRTVINGKRYFTTLFVVVVLLATTDVVFAIDSIPAVF
                     GISHNTIVIYTSNIFAVLGLRSLFFLLHGAIGRFAHLQQGIAVVLIFIGIKMLVEFFD
                     VEIPVYLSLLVILGCIAGGIIYSVWEGRRKSEGGDGTRR"
     gene            complement(4691..7171)
                     /gene="gldG"
                     /locus_tag="VGQ51_09465"
     CDS             complement(4691..7171)
                     /gene="gldG"
                     /locus_tag="VGQ51_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HEV9036839.1"
                     /translation="MWAICKKELRQFFSSLTGYIAIIVFLLLNGLLLFVFPDTDILAF
                     GYATLDKFFELAPWILLLLIPAITMRSFSEEFRTGTYETLQTVPLGRSGLIGGKYLAS
                     LIVVVIALVPTLVYFLCIQRLSGQGGIDTGATTGSYLGLFFLAAVFTAIGTWCSSWTT
                     NPVVAFIVAAFACFILYSGFGAISTLPVFTAGLDYYIGMLGIDFHYRSISRGVVDLRD
                     VLYVGSVILLFLYLIGRHLAAREGVTRRHLAAREGVTRRHLAARERRMGVVGPIVLVV
                     VLVGVNLLASHLHYRIDLTKDGRYTLSEPTKTLLGRVDDEIQIDLFLAGDLKPGFRKL
                     ATSIDDEIREFNEFCGGKIRLHAYDPLANLDDSAKSQLLDSMGQMGIQPYNQIVQAKK
                     GAEESQRIILPAAIVHYQNKIYPINLLKGVHVEGEEQMYTNAETQLEYKFGSAIDKLI
                     TKEEPMVGYVMGNGEPLDYRVYDLIEDLRHNYRFGIVALDSQAVIPLKYSALIIVKPT
                     SKFTDREKLALDQYVLHGGQIIWAVDVLHAEKDSLRQEQGTVAYDRGLELEDLFFKYG
                     VRINEDLIEDRQCASLNMVVGMQGTKPQFEALPWPYFPLLVGSLTHPISKNLDPVMAQ
                     FANSIDTVKAPGIRKTVLLQSSANARVVGTPAIISFDVLKYKDDPKMFPLANIPAAVL
                     CEGKFQSLFENRIASEVADSLSTVYKEPFVAVGEKPSRVIVVSDGDIFMNEVSQRGPM
                     PLGYSEGNGGYTFANQDFIDNAVEYLVNPSRILETRAKDFTLRMLDPTKVENDRTLWQ
                     LINIGLPILLVVLGGYVYQAVRRRRFAR"
     gene            complement(7258..>7460)
                     /locus_tag="VGQ51_09470"
     CDS             complement(7258..>7460)
                     /locus_tag="VGQ51_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036840.1"
                     /translation="SGEWGNLLNQKTRLKMSGFFVSRWFRLNAFYVDPLTGRYLSSDG
                     WQVEHANIGIPIFWQSFITLNF"
BASE COUNT         1842 a         2447 c         1591 g         1580 t
ORIGIN      
        1 ggcctccggc aacggcaccg ccattcttac cctcgtcgat ctgtatggac gcgtgatccg
       61 ccgccagcaa caacaggtaa cacagggcta caacaccgtc acgatgtttg gcctgtccgg
      121 cctgcctgtc gcaacctatg cgctgcagat ccagtacaac aatcaattgg tcagtgaaaa
      181 aatggtgaaa gtaaacgtgc aatagggcgt tccccgcctc cccgcgctgc tatctcccgc
      241 gcgctccggc gggtcgggct ctctgtcgcg cacggcgctc ctgccgatcc ctaacgcgat
      301 ccggaagcct taaacttgca ccgcatccac cttcgcctgc ccctctacgg gatggttctc
      361 gacgcccggc cgcttgtgaa agaatttctt ttggaagatc aggatggcga tcaccccgac
      421 cgagatcgaa gcatccgcca ggttgaagac gggactgaaa aactcgaact cctggccccc
      481 tacaaaggga aaccatccgg gataatgcgt cttgatgatg gggaaataaa gcatatccac
      541 gacacgcccg tgaagaaagg tcgtatatcc gtgcggcggg aatatcttag ccacatgttc
      601 ataggtgctg cgctcgaaga tgagcccgta aaacatgctg tcgagcagat tgccgagcgc
      661 ccccgcaaag atcagccccg cacagatgat aaacccgcga tgatactttt tcttgatgat
      721 ggtctgtata taataaacgc cgaataccac cgccactagc cggaagagcg tcaacgccat
      781 cttcccccag ctgccgccga acttccagcc ccatgccatc ccttcatttt cgacgaaata
      841 gatctgtgcc ttgtcacccg ccaaccttat cccattgtca taaggcgata tcttttccat
      901 gtaagtgtcc accggatgac tcaacggcat atgcgtcttt acccagatct tcagcgactg
      961 atcggctacg atgatcagca gtacgagtag aaatattgtc cttgccttca ttaggtgcca
     1021 aatatactta atccttccca tgaaaaccgt taaaatacgc gggcggcgtc ccttgcccct
     1081 cctcgttctt acccttcact ttgcttggct tcgtccctca ttactcatcg gccgaacctt
     1141 cgcgtcccgc attactcttc ataatatatc cttttcaccc gctgcgacac acccgtcatt
     1201 atctcataag gtatcgttcc cgcccactta gctaactggc tgaccgataa ttcgttgcca
     1261 aacaccacca cctcatctcc ctcactcacg cccgggatac ccgtaatatc gatcatcgtc
     1321 atatccatac tcaccgcacc gatcgtcggc gccagatgac cgcgaaccca catcttgcca
     1381 acaccatttc ccagcgccct gacatagccg tctgcatatc cgatgcggac agtcgcaatg
     1441 atcgtgcctt ccgttgcctg tccaagacga ttatatccta ccgtatcacc cgtgtgaagc
     1501 ctcttgatct gcgcgatagt agtcttcaat gtcgacacct cctccagctg taatctcccc
     1561 gatcccgcac tgtcgatgcc gtagagaccg ataccaagcc tgaccatatc catttgcaac
     1621 cctggatgcc gtacgatacc cgccgaatta tccatatgcc gaaggaatga ataacccaac
     1681 gccttaccga tccggtccgc catctcatga tacaactttc cctgcccgcg cgtgaaccca
     1741 tcctgttccg catcttcact cgcggcgaaa tggctgaaga cgctcatcac ccggaaggcc
     1801 gccgtcccca acgctgccgc gagcacatcc atctcctccg gcggaaaccc gagacgattc
     1861 atgccggtct ccagttccag gtgtaccgga aaagccggaa taccttcctt cttcagccag
     1921 cgatcaaaat cctgcaacag cgcgggagaa tagatcaccg gttcgagatt gtattccacc
     1981 agcgagtcga acgagctcgt ttccgtattc atgatcatga tcggcacacg aatacccgcc
     2041 ttccgaagga ccacgccctc atcggcatac gccacaccca gataatcgat cccatgaaat
     2101 tgcagcagac cagcgatctc tgcactcccg ctgccatagc cataggcctt caccatcgcc
     2161 atgatacgcg tcgcaggatc cagcacctgg cgataagcac gcagattcgc cgccatggcc
     2221 cccaggtcca cctccatgac ggtctggtgg atctgttcgg tcagcagcct gtctatcttt
     2281 tcgaactcga acacccgcgc ccccttgatc aggatcgtct catcgcggaa atggagcctt
     2341 gacaggtcgt cgctgaaagc ctcgacggta ggatagaaga tccgttctcc cgggaaccag
     2401 gtttcgaact ccgatgtatg cgcgccgata tgtgtgccga tgccgatcag ccggtccacc
     2461 gccttttgcc ccaatgcccg cgcgatcagc gcatatagtt cgccctcttc ccggccgctc
     2521 tcgagaagat ccgagaggat gaccgtgcgc cgcggatgct gttgctgctg cgaaagaaaa
     2581 tccagcgcta tcgacagcga gctgagatcc gcgctgtagc tgtcattgat gatcgaacaa
     2641 tgattgatgc cattcttcaa ttccaatcgc atcgcgatcg gcgcgagaag ccgcattcgc
     2701 tccctgatct tctcctccgg cactcttaaa aacaacagca cggcaacgca atgcagagca
     2761 ttctccaccg aggcatgatc actgaatggc aactccaccg caaatactgc attaccttcg
     2821 cctccggcac cccctgatcc aactccgatc ttcgcccacc cgtctccttt ctctaccgga
     2881 ccgatacgca ccgcatttcc ttctcccctt ccccacgccc atcgccgagc cttcccttcc
     2941 caccgctcca cggccttcac cgtctcttcc tgatcggcac aatacaccaa cacctccgcc
     3001 ctcacgaata atttcaattt ctccgccgcc ttttcagcaa gactgccaaa accctcgcta
     3061 tgcgcctcgc cgatattggt aaacacgccg atggtcggac gaaccatcgg ctcgagcctg
     3121 ctcatctccc cgcgccgcga aatacccgcc tcgaaaatgg ccagctgatg cgccggcccc
     3181 agctgcgcca cgctcaacgc cacacccgtc tgcgagttat agctcttcgg actccgcgct
     3241 atctgacagt cttcttcaag cagcgtattg agccactcct tcacgatcgt cttgccatta
     3301 ctacccgtta tcccgatgac cggcagcgca aaatgtttcc gatgcgcggc taccaaagtc
     3361 tgcaacgcca ccagcgcatc acccacccca atgatattcg cccccggcaa ctccgatccc
     3421 tcccacccct cgccaacgac aaaattccgg acaccgcggc gatacaattc ctccacatac
     3481 cgtcccccat cccttcgcgc ccctttcaac gcaaagaaca acgtcgacac agggaagatc
     3541 agccggcggc tgtccagcaa cagctgcgat accgcatcgt ccgcatagaa cctgatccat
     3601 cgtcccccga tcacttcatt gacgtatgtt atgctatatt ccatcctgat tattacccga
     3661 ttctcgccgg cgatcccatc ccggcctcca ggtttccgcc gcgcatccca tcccggcctc
     3721 cccatcaccg ccgcgtccca tccccgccct ccgatttccg ccgcccttcc caaactgaat
     3781 aaataatacc gccggcaata cacccaagta tcaccagcag cgaaagatac acagggatct
     3841 ccacatcgaa gaattccacc agcatcttga tcccgataaa aataagcacg accgcgatgc
     3901 cctgctgcaa atgcgcaaac cgcccgatcg ctccatgtaa caggaagaac agcgaccgaa
     3961 gccccagcac cgcaaagatg ttcgaggtat agatcacgat cgtattatgc gagatcccga
     4021 acaccgcggg tatgctgtcg atagcaaaca ccacatccgt cgtggccagc aataccacca
     4081 ccacgaaaag ggtggtaaaa taccgcttgc cattgatgac agtccgcatc ctgccgtccc
     4141 cgtcactacc cgtaaggggc aatagccggc gcatgaattt atagaccttg ttctcctcca
     4201 cgtccatccc ggcctccctg tcggcagtga acatcgttat cccagtatag atgagtgccg
     4261 cgccaaacac atacaacagc caatgaaaac gcgtcaccag cgcgatgccc gccgtaatga
     4321 agatgatccg gaaaaaaatg gcgagaagga tgccgatcag cagtacgcgc gcataatact
     4381 gcggcttcac acggaaaaag gaaaagatga ggatgaaaac gaaaatattg tcgatgctga
     4441 gactccactc catcagatac gcgctcacgt actccagcgc gatcttctgc ccgtcctcat
     4501 accatagaaa cccgaaaaag accattgcca gcacaaccca gaaaaccgtt tggaacagcg
     4561 ccttgcggat ggtgacggga gcgttctttt tgctcagcag cccaagatcg aaaaggatag
     4621 ccagaaaaag gagaatgccg aaagtaagat aggtgatatc cgttgggctc atatcacaaa
     4681 tttacgtttt ctatcgcgca aaccgccgcc ttctcaccgc ctgatacaca taccccccca
     4741 acaccacgag caggataggt aacccgatat tgatcagctg ccacagcgtc cggtcgttct
     4801 cgaccttagt cggatccagc atccgcaacg taaaatcctt cgccctcgtc tccaggatcc
     4861 tcgacggatt caccagatac tccacggcat tgtcgataaa atcctggttg gcgaaggtat
     4921 agccgccatt gccctcgctg taccccagcg gcatcggccc ccgttgagat acttcattca
     4981 taaagatgtc cccatccgag accacgatca cccgcgacgg cttctcccca accgcgacaa
     5041 acggctcctt atagactgtt gaaagactat ccgccacctc gctcgcgatc cgattctcaa
     5101 acagcgactg gaacttaccc tcacaaagga cagccgccgg tatattggcc agcgggaaca
     5161 tcttcgggtc atccttatat ttcagaacgt cgaacgagat gatcgccggc gtacccacta
     5221 cccgtgcatt cgccgacgac tgcagcagta ccgtctttcg tatacccggc gccttcaccg
     5281 tatcgatcga attggcaaac tgcgccatta cagggtccag gttcttcgaa ataggatgcg
     5341 tcagactgcc gaccagcagc ggaaaatatg gccacggaag agcctcgaac tgcggcttgg
     5401 tcccctgcat gcccaccacc atattcagac tggcgcattg ccggtcctcg atcaggtcct
     5461 cgttgatccg gacgccatat ttgaagaaaa ggtcttccag ctccaacccg cggtcatagg
     5521 cgaccgtgcc ctgctcctgc cgcagactgt ccttctccgc atgcaacacg tccaccgccc
     5581 agatgatctg accgccatgc aatacatatt ggtcgagcgc gagcttttcc cgatcggtaa
     5641 acttcgaagt cggctttaca atgataagcg cgctatattt taaaggaata acagcctggc
     5701 tgtccaaagc cacgatgcca aaacgatagt tgtgccgcag atcctcgatc agatcgtaaa
     5761 cacgatagtc cagcggctcg ccattgccca tgacataccc gaccatgggc tcctccttcg
     5821 tgatcagctt gtcgatggcg cttccgaatt tatattcgag ctgtgtctcc gcattggtat
     5881 acatctgttc ctcgccctct acatgtacac ctttaagcag atttatcgga tagatcttat
     5941 tctgataatg cacgatagcc gccggcagta tgatccgctg actctcctcc gctccctttt
     6001 tcgcctgcac gatttggtta tacggttgaa tgcccatctg acccatcgaa tccagcagct
     6061 gcgacttggc agaatcatcc aggttggcga gcgggtcgta tgcatgtaac ctgatcttcc
     6121 ccccacagaa ttcattaaac tcccggatct catcatcgat gctggttgcc agcttccgaa
     6181 accctggctt gagatcaccc gcgagaaaaa gatcgatctg tatctcatca tcgacccgtc
     6241 ccagcaaagt cttggtaggc tcgctaaggg tatagcgccc gtctttggta agatcgatcc
     6301 ggtaatgcag atgtgacgcc agcaaattca cccccaccag cacgaccacc agtactatcg
     6361 gtccaaccac gcccatccgc cgctctctcg ccgccagatg cctccgcgtc actccctctc
     6421 tcgctgccag atgcctccgc gtcactccct ccctcgccgc cagatgccgc ccgatcaaat
     6481 acaggaataa taagatcacg ctccccacat acagcacatc gcgaagatcc accaccccac
     6541 ggctgatgct acggtaatgg aaatctatac ccaacatccc gatataataa tccagacccg
     6601 ccgtgaacac cggtagcgtg ctgatagccc caaacccgct gtacaaaatg aaacacgcaa
     6661 aagcggccac gataaacgcg accaccggat tagtcgtcca gctgctgcac cacgtgccga
     6721 tcgccgtaaa caccgcagcc agaaagaata accccagata gctgcctgtc gtcgccccgg
     6781 tgtcgatacc gccctgcccc gagagacgct ggatacaaag aaaatagacc aaagtcggca
     6841 cgagcgcaat caccacgacg atcagactcg ccagatattt ccctccaatg agaccactcc
     6901 ttcccaacgg aacggtctgc aacgtctcgt aggtgcccgt ccggaactcc tccgaaaaac
     6961 tgcgcatcgt gatcgcaggt atcagcaaca acaatatcca gggtgccagc tcaaaaaatt
     7021 tatccagtgt ggcatatccg aaagccagaa tatccgtatc ggggaaaacg aataataaaa
     7081 gcccgttgag caacaggaaa acaatgatgg cgatatatcc ggtaagactg ctaaagaatt
     7141 ggcggagttc ttttttgcaa atagcccaca taagcgcgaa aatatataat atatacgtcg
     7201 accttcattt cttattgtct gccagacata tatcaattac aaaaatgtga ataagtgtta
     7261 aaaatttaat gttataaaac tttgccaaaa aataggaatg cctatattcg catgctctac
     7321 ctgccaacca tccgatgaaa gatatcttcc ggttaaagga tcgacgtaaa aagcatttaa
     7381 ccgaaaccac ctgctaacaa agaatccgct catcttgagg cgggtttttt gatttaagag
     7441 gttcccccac tcacccgatc
//
LOCUS       DASYSV010000317         4765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1859081, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000317 DASYSV010000000
VERSION     DASYSV010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859081"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..651
                     /locus_tag="VGQ51_09475"
     CDS             166..651
                     /locus_tag="VGQ51_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036841.1"
                     /translation="MKDLLLIVVAFGTILLVWNAGFYKQWFAEKPAQYWSDFWKEKED
                     TASLDAIRKERYGVIYTISEKVKEVMEKKKIAHPVILFEPNSYYRDSLHIYPSFRAPE
                     PAVFYYYTGLESVWINSPDVNKANFLVRLSKKGVTLEQISSPRQLQQILDRYKKYTPI
                     L"
     gene            656..2392
                     /locus_tag="VGQ51_09480"
     CDS             656..2392
                     /locus_tag="VGQ51_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036842.1"
                     /translation="MQVLAFLLLLCIHLVTGYGILRLFRLRLKKARLITVSLLLGIAA
                     ASFLPFLLQLLFIPITGATVFGSLTLAALLLNIPTIIRIRKEGFSAMLRRLSPGRLHV
                     RPYEVVYWMGIGFLVFVSVWRCYYYPPVSRDALSGPEAIAEFTVREHTMVNSFFNIDL
                     QSTNNQFKSPFLVSLQVVCKLAGFPFGQVWLSIVFVSLTVFLYNALREKLHPLLAATL
                     LLLFTMAPEPYAYTFMILYDYSNMVYFFLGLYFLFQYFSATRMSAGATAGQPAAHAPP
                     ITSPPAAHAPPVTGPPAAYTPPVTGRPSGSFTDHVHPAAYPPAGSIPRGSFYLAGLLL
                     GIATYIRSETLVLVLLFFPPILLMQRRGRFAFGKVLVNDLLFLLPTLVGYYLPVQLYN
                     NHYLPVHYVVGVLVDPHLALPGLFRRYADIFTRLLTGELSVRLWGYFYYLTAVLLVAE
                     ALVVRRFNRDALNWLYAMAVVYLGLGFIGWLLPVMDLTDSTKRGLFKLLPLALFYLAN
                     NSLLKRFSVLIARWEQTAPARRSSRSAEAKPKAVLTKSGKLTPTGAPSRNVTPATPAP
                     SRSTPKKLRRKK"
     gene            2516..4072
                     /locus_tag="VGQ51_09485"
     CDS             2516..4072
                     /locus_tag="VGQ51_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEV9036843.1"
                     /translation="MLVKTFGSAVYGVEAITITVEVNVTEGLHTFIVGLVDSAVKESI
                     QRVESAIKSNGYYMPRTKIIINLAPAGIRKFGSAFDLPIAIGILAASDQLPDPEQLKD
                     YVIMGELGLDGTIQPIKGTLPIAIQTAKENFKGLIVPHQNASEAAIARRLKTFAFTHL
                     REVILFLKDNGSSTPIVSDIRNGFLPDDLPSDIDFSEVKGQESIKRALEITAAGGHNA
                     ILVGPPGSGKTMLARRLPTILPPPSFREALETTKIHSVAGKLPPNMPLLSQRPFRSPH
                     HTISGMALVGGGSIPTPGEISLAHNGVLFLDELPEFSRSTLEVLRQPMEDRRVTISRA
                     KFSVEFPSSFILVSSMNPCPCGYYNHPDKACSCTSGAVSKYLNRISGPLMDRIDLHVE
                     VVPVPYERLSASQPGESSRQIRQRVMAAREIQRQRYADGFDQMEKGTCFNARINGRLL
                     QQFCRIDKTGDQLLRTAMERLKLSARAYDHILKISRTIADLAGKEDIAPEHIAEAIQF
                     RSLDREEWVG"
     gene            complement(4084..>4765)
                     /locus_tag="VGQ51_09490"
     CDS             complement(4084..>4765)
                     /locus_tag="VGQ51_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV9036844.1"
                     /translation="MRRYHFAFPVFIEGEDPFVEATQLYHLLLPKPVAYDVRLDRDSN
                     FLFLTGANMAGKSTFIRSVGVVVFLAHLGMGVPAQSMRLTVFDGILSNINVEDNIGKG
                     ESFFFNEVQRIRNTLEKIGNRKKWLVLIDELFKGTNVQDAMKCSSTVIKGLIRIRRSL
                     FILSTHLYEIGDELKQYPNILFNYFETAVKDDQLEFSYQLKPGISNDRIGYLILKREK
                     VVEMLEKL"
BASE COUNT         1052 a         1433 c         1133 g         1147 t
ORIGIN      
        1 gctgatcgtt caatcactgg tccatcgcaa cagccccgtc ccggcggaca acctgaccat
       61 cgacggcaga gcccttgccg cgaccaataa aaaaattgcc tgagctctcc cgatttcttt
      121 tgatttgccg gcattttaac tagttttagc gtctagcaga tgcttatgaa ggacttattg
      181 ctgatcgtcg tcgcctttgg aacgatcctc ctcgtctgga atgccggctt ctacaaacaa
      241 tggttcgccg aaaagccggc tcaatactgg tcggatttct ggaaggaaaa ggaggacacc
      301 gccagcctgg acgccatccg gaaagagcgt tatggcgtca tttataccat ctcggagaaa
      361 gtaaaggaag tcatggagaa aaagaagatc gcacatcccg tgatcctctt cgaacccaac
      421 agctactatc gcgactcctt acacatctat ccgtcgtttc gtgcccccga accggccgtc
      481 ttttattatt acaccggtct ggaaagcgtc tggatcaact cccccgacgt gaataaagcc
      541 aatttcctgg tccgtctttc gaagaaaggg gtcaccctcg agcagataag ctcaccccgg
      601 caattgcagc aaatcctcga tcgctacaaa aaatatacgc caatcctata aacctatgca
      661 ggttctcgcc tttttgctgt tgttatgcat ccacctggtc accggctatg gcatccttcg
      721 ccttttccgt ctccggctca agaaggccag gctcatcacc gtttcattgc tgctcggaat
      781 agccgcggcc tccttcctgc cattcctgct acagctgttg tttatcccca tcaccggcgc
      841 taccgttttc gggtccctga cgctggccgc gctgttgctg aatatcccaa cgatcatccg
      901 cattcgcaaa gaaggctttt cggccatgct ccgacggttg tcccccggtc ggctccatgt
      961 ccgcccatat gaggtagtct actggatggg gatcggtttt ctcgtctttg tcagcgtgtg
     1021 gcgctgctat tattatcctc ctgtttcgcg cgacgccctt tcggggcccg aggccatcgc
     1081 cgaattcaca gtccgggaac acaccatggt aaattccttt ttcaacatcg atctgcagtc
     1141 gaccaataat caattcaaat cgcctttcct cgtctcgctg caggtcgtct gcaagctggc
     1201 cggctttccc ttcggacagg tgtggctgag tatcgtgttt gtcagcctga cggtttttct
     1261 ctataacgcc cttcgcgaaa agctccatcc cctcctcgcc gcgaccctcc tgctgctctt
     1321 caccatggcg cccgaaccct atgcctacac cttcatgatc ctctacgatt acagcaatat
     1381 ggtctatttt ttcctgggac tgtatttcct gtttcaatat ttttccgcca cccgaatgtc
     1441 ggctggtgcc accgccggcc agcccgctgc tcacgcacct cccatcacca gcccacccgc
     1501 tgctcacgcg ccccccgtca ccggcccacc cgctgcttac acgcctcccg tcaccggccg
     1561 ccccagcggc tcgttcaccg accacgtcca tcccgcggct tatcccccgg ccggcagcat
     1621 cccccgcggc tctttctatc ttgccggttt attgctgggc atcgccacct atatccgttc
     1681 cgagaccctc gtcctggtcc tgctcttctt tcctcccatc ctgctaatgc aaagacgagg
     1741 gcgattcgca tttggaaagg tactagtgaa cgatctcctc tttttattgc ccacgctggt
     1801 aggttactat ctgcccgttc agctctataa caaccactac cttccggtgc actatgtagt
     1861 cggcgtcctc gtggaccccc atcttgcctt gcccgggctg ttccggcgct atgcggacat
     1921 ctttacgcgt ctcctcaccg gtgagctttc cgtgcgtctt tggggctact tctattacct
     1981 gacggccgtc ctcctggtag cggaagcgtt ggtcgtgcga cgtttcaaca gggacgcgct
     2041 caactggctc tatgcgatgg cggtagtata tcttggtctc gggttcatcg gctggctcct
     2101 gccggtaatg gatctcaccg attccacgaa aagaggcctc ttcaaattgc ttcccctcgc
     2161 actcttctat ctggccaaca acagcctgct gaaacgtttt tcggtgctca tcgccagatg
     2221 ggaacagacc gccccggccc ggcgctcttc ccggtccgct gaagcaaagc ccaaagccgt
     2281 tcttactaaa tctggtaaac tgacccccac cggggcgccc tcccgcaacg ttacgcctgc
     2341 tacccccgca ccctctcgct caactcccaa aaaactgcga cggaaaaaat aaacggcccg
     2401 gtaccctctc ttaccctacc ctcacaaacg ggtggaacaa tttcgttcat cggcgcatag
     2461 atttttccgt cggcaataaa aataatggct agcttcatcg gctaaacctt gtcctatgct
     2521 ggtgaagaca tttggaagcg cggtttatgg agtagaggcg atcaccatta ccgttgaagt
     2581 gaatgtgacg gaaggcctgc atactttcat cgtcggtctg gtcgacagcg cagtgaagga
     2641 aagcattcag cgggtagaaa gcgcgataaa aagcaatggc tattatatgc cgcggaccaa
     2701 gatcatcatc aatctcgctc ccgccggcat ccgaaaattc ggttccgcat tcgatctccc
     2761 catcgcaata ggaatactag ccgccagtga ccaactgccc gaccccgagc agctgaaaga
     2821 ctatgtgatc atgggagaac tgggacttga cggcaccatt caaccaatca aagggacact
     2881 acccatcgct attcaaaccg caaaggaaaa tttcaaagga ttgatagttc ctcatcagaa
     2941 tgcatccgaa gccgccatcg cccggcgatt gaagactttt gcctttaccc atctccggga
     3001 agttatcctt tttttaaagg acaatgggtc atcgactccc atcgtctcag atatccggaa
     3061 cggctttctt ccggatgatc tgccttccga tatcgatttc agcgaagtga aaggacagga
     3121 gagcatcaag cgggcgttgg agataacggc cgccggtggt cacaatgcca tattggtggg
     3181 gccacccggc tccgggaaga cgatgctggc gaggcgactg cctactatcc tcccacctcc
     3241 cagtttccgg gaagcgttgg aaacgacaaa gatccatagc gttgccggca aattgccgcc
     3301 caatatgccc ctgctttcac aacggccttt ccgcagccct catcacacta tctccgggat
     3361 ggcgttggtg ggcggaggca gcattcccac gccgggggag atctcccttg cccataacgg
     3421 ggtgcttttc ctggatgagc ttccggaatt cagccgcagc acactggagg tcttgcggca
     3481 gccgatggaa gacaggagag tgacgatcag cagggcaaag ttttcggtcg aattcccttc
     3541 ctcctttata ctggtttctt cgatgaatcc ttgtccctgc ggatactaca atcatccgga
     3601 taaggcgtgt tcctgtacat ccggcgctgt ttcaaaatat ctcaaccgta tatccggtcc
     3661 attgatggac aggatcgatc tgcatgtaga agtggtgccg gtgccctatg aacgattgtc
     3721 ggcaagtcaa cccggggaaa gtagcagaca gatacgtcag cgtgtaatgg cggcaagaga
     3781 gatccagcga cagcgctacg ccgacggatt cgatcaaatg gaaaaaggga cctgtttcaa
     3841 tgcccggatc aacggtcgcc tgctccagca attttgccgc atcgacaaga ccggcgatca
     3901 attgctgcgt accgctatgg agaggctaaa gctgtcagca agggcctacg accatattct
     3961 gaaaataagc cgaacgatcg ccgatctggc aggaaaggaa gacatcgctc ccgagcatat
     4021 tgcagaggcc attcaattca ggagcctgga tcgggaagaa tgggtcggat aagtgcagcg
     4081 cgatcacaat ttctccagca tttccaccac cttttcccgt ttcaatatca aatacccgat
     4141 ccgatcgtta ctgatacccg gttttagctg atagctgaac tccagctgat catccttcac
     4201 cgccgtctcg aaataattga acaggatatt cgggtattgt ttcagctcgt ccccgatctc
     4261 gtacaaatgg gtggagagga tgaacaacga tcgcctgata cggatcagcc ccttgatgac
     4321 cgtactcgaa catttcatgg catcctgtac attcgtgccc ttgaacaatt cgtctatcag
     4381 caccagccat ttcttgcggt tgccgatctt ttcgagggtg ttgcggatac gctgcacctc
     4441 attgaagaag aagctttccc ctttgccgat attgtcctct acattgatat tgctgaggat
     4501 tccgtcgaac acggtcaatc gcatcgattg tgccggcaca cccatgccga gatgcgccag
     4561 gaagacgacg acacctacgc tccggatgaa ggtgctcttg cctgccatat tcgcgcccgt
     4621 gagaaatagg aaattcgagt ccctgtccag ccgaacgtca taggccacgg gtttgggcaa
     4681 tagcaaatga taaagctggg tcgcttcgac gaacgggtct tcgccttcaa taaaaaccgg
     4741 gaaggcaaaa tgatatcgcc gcatg
//
LOCUS       DASYSV010000318         6461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_701471, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000318 DASYSV010000000
VERSION     DASYSV010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701471"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VGQ51_09495"
     CDS             <1..766
                     /locus_tag="VGQ51_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV9036845.1"
                     /translation="QTLKIFLLFNKLSAHKKILYDALIASLGEYAVVDFYIYNNDFGL
                     FKKLLSDLKEDYSHYVIIPHFLEGGERAPDIINSLPKDKLLVLDKKIPGITGEFAAVY
                     QNFGQDIYSALEKARDQLGKYHTVKIIFPSHTYQPVEILQGCSRFCQEYAFSYKVVHS
                     IADEPIGEGEVYINLMEEDLVVLIERIISLKLAVGREVGVISYNETPLKKIILNGITT
                     ISTDFREMGVMAARQILENTHIQEEVPFYLTLRHSL"
     gene            complement(786..1766)
                     /locus_tag="VGQ51_09500"
     CDS             complement(786..1766)
                     /locus_tag="VGQ51_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9036846.1"
                     /translation="MKPTVWGILGPGKIARKFARALTMVEGARLGAIASRDAARAAAF
                     AKEHGAERTWLHYEELASDPGIDAVYIATPHGFHAEHSLLCLQQGKGVLCEKPMALSA
                     LQVGAMIEASRIHQAFLMEAMWTRFLPLTREVLALVASGRIGTVKYVRADFGFLAPFN
                     ETSRLYDLRLGGGSLLDIGIYPLYLCLQLLGEPDGIVAAGRLSPTGSDETCHALLQYA
                     HGATAVVSSTLACQTSLTAEIAGTEGMIHIPGPWYRNDRFEWNRTGEAVQQVQLEPMV
                     NGFEYQIREAMECMEQGRIESLMLPHSFSLLMSRTMDEIRRQIGVVYPGE"
     gene            1798..4119
                     /locus_tag="VGQ51_09505"
     CDS             1798..4119
                     /locus_tag="VGQ51_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9036847.1"
                     /translation="MIRVTLLLLLSSIGVNVSAQTHFTVSGTIRDKKTGEILIGASVS
                     LLEIPRSGIASNAYGFYSISAPPGKYTLLVSFSGYEPDSIPVRLDQNIGLPVDLATAG
                     GALTEVVVSAQKKNENVTRALMGVQKLTTREIRNVPVLFGEKDVLKTIQLLPGIQFAG
                     DGNSGFYVRGGGLDQNLILLDEATVYNPSHLLGFFSTFNSDAIKDITVYKGGMPAEYG
                     GRLSSVVDIKMNDGNNKEYHFGGGIGLISTRLNAEGPIVKDRGSFSISGRRTYADVFL
                     KISPDSSVNQNSVYFYDLNIKANYRFDENNRVYLSGYFGRDNLSFANTFGIDYGNATG
                     TARWNHVFNSRLFSNTSLIYSKYSYKIKINSANNDIGFTSNIRDVNLKEDLQYFVDAG
                     NKIDFGFSVIHHAMAPGIISASPGSSFNPLTLQNKYSLENALYISHEWSPSDRWHLNY
                     GLRASYFLVLGPGKFYTYDSSGNTKDSTQYNSGSIVTTYTNPEPRASLSFQINQTSSL
                     KISYNRNSQVLHLLSNSTSSNPTDLWIPSSNNVKPEIADQESIGYFRNLRDDRYEFSV
                     EAYYKSMQNQIDYRNGAELIANENVESQLLFGKGRAYGLELFLKKKAGKFTGWISYTL
                     SRTELKIDGINRNNWYPAKQDRTHDVSLVGIYQASPKWTLSATWVYYTGSAVTFPNGK
                     YQVGGMTAFYYTERNAYRMPAYHRMDVAATLQGKKKKKFESSWTFSVYNLYGRENAYS
                     IIFQNDPDDPSKTQAVQYALFRFVPSVTYNFKF"
     gene            4123..4920
                     /locus_tag="VGQ51_09510"
     CDS             4123..4920
                     /locus_tag="VGQ51_09510"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEV9036848.1"
                     /translation="MKRPTLLLLAICALAIAACKKVIQVDLNSVAPQIVIEGEVTNGP
                     APYFVRISKTVDFSASNDYPPVTDAVVLLTDTTNGISDSMVEASPGLYKSIEVGGVPH
                     HTYRLDVTIEGKQYTALSTMPERVKLDSVTFAQNIGFDNKLEINAVAGFQDPPGIADY
                     YQLSETVNSRLIPDIFVLEDRLSDGRYIRYPLYNDSAYFAPGDTLRLTMNCIDRNIYN
                     YFFQLMNVAGNNNFQSATPTNPATNIKGGALGYFSAHTTEKVDIIVY"
     gene            complement(4947..6371)
                     /locus_tag="VGQ51_09515"
     CDS             complement(4947..6371)
                     /locus_tag="VGQ51_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV9036849.1"
                     /translation="MKRILIIDDDMDMCNLLGRFLQKKGFETEASHSGGKGIQKFKES
                     KFDVVLCDFRLGDKEGREVLREIKNVDPYAIVIIITGYSDIKTAVDVIKAGAFDYITK
                     PLIPEEVLNVIGRALQQSEAGDTPKPTTAVQAMRKPGRNIPSDDEFLVGQSPATKELY
                     RQIELVAPTNYSIILYGESGTGKEVIARTIHQNSARKDKPFVAMDCGTLSKELAGSEL
                     FGHVKGAFTGALGDKEGHFELANGGTLFLDEVANLSYEIQAALLRVIQERKFKRVGGV
                     KEMETDVRIIVASNENLQEAYRKGRFREDLFHRFNEFSIVLPAFRHRKEDIPLFAEFF
                     LSKANKELNKEVEGFEPEVMQIFMNYPWPGNLREFRNVIRRAALLTSSGRVNARVLPA
                     EIVDTNTYSQNFIPQQLDVPIVAAPHHPIAHKDTDLKNAAAQAEYDTIMNVLKQVNYN
                     KSRAAEILKIDRKTLYNKIKSYQP"
BASE COUNT         1532 a         1766 c         1660 g         1503 t
ORIGIN      
        1 ccagaccctc aagatcttcc tgctattcaa taagctcagc gcgcacaaaa agatactcta
       61 cgacgcattg atcgcctcgc tgggggagta tgccgtcgtc gatttctaca tctacaacaa
      121 cgatttcggg cttttcaaga aactgctcag cgatcttaaa gaggactatt cgcactatgt
      181 catcatcccc catttcctgg aaggcggaga aagggcgccc gatatcatca atagtcttcc
      241 caaggacaag ctgctggtgc tggataaaaa gatccccggc atcaccggtg aatttgcggc
      301 cgtgtaccag aatttcggac aggacattta ttccgccctt gaaaaagcgc gcgatcagct
      361 gggcaaatat catacagtca agatcatctt cccgagccat acctatcagc cggtggagat
      421 actacagggt tgcagcagat tttgccagga atatgccttc agctacaagg tggtgcattc
      481 gatagccgac gagccgatag gggagggaga ggtgtatatc aatctcatgg aagaggacct
      541 tgtcgtactg atcgagcgga tcatctcgct gaagctggct gtgggccgcg aggtcggcgt
      601 gatctcctac aacgagactc cgttgaaaaa gatcatcctg aatggcatta cgacgatatc
      661 caccgacttt cgggaaatgg gcgtcatggc ggcgcggcag atactcgaaa atacccatat
      721 acaggaggag gttcctttct acctgacgct gcggcattcg ctgtgaggca gcgcggtggc
      781 tccggttatt ctcccggata gacgacgccg atctgccggc gtatctcatc catcgtcctg
      841 ctcatcagca ggctgaagga atgcggaagc ataagacttt cgatccttcc ctgttccata
      901 cattccatcg cctcgcggat ctggtattca aaaccattga ccatcggttc cagctgcacc
      961 tgctgaacag cctcgcctgt cctgttccat tcaaaacgat cgttcctgta ccagggcccg
     1021 gggatatgga tcatgccttc cgttcccgcg atctccgccg tcagcgaagt ttgacaggcc
     1081 agcgtgctgc tcacgacggc ggtagcgcca tgggcgtatt gcaacaaagc atgacaggtc
     1141 tcatcgctgc ctgtcggcga caggcgtccc gcggcgacga tgccatcggg ttcgcctaat
     1201 aattgcagac agagatacaa cggatagatg ccgatgtcga gcagcgaccc gccgccgagc
     1261 cggagatcat agagccggct cgtttcatta aaaggcgcca aaaatccaaa atcggcgcgg
     1321 acatatttta ccgtaccgat gcggccggaa gcgaccaggg cgagaacctc gcgcgtcaaa
     1381 ggcaggaatc ttgtccacat ggcttccatg agaaaagcct ggtgaatacg ggaggcttcg
     1441 atcatggcgc cgacctggag ggccgacagc gccatgggct tttcgcaaag cacgcccttc
     1501 ccctgttgca gacaaagaag gctgtgttcc gcatggaaac catgcggagt ggcgatatag
     1561 accgcatcga tgccggggtc cgaagcgagc tcttcgtagt gtaaccaggt cctttccgcg
     1621 ccgtgctcct tagcaaaagc ggcagccctc gcagcgtccc tggatgcgat ggctcccaga
     1681 cgggctcctt cgaccatggt gagcgcacgg gcgaatttcc gggcgatctt tccgggaccg
     1741 aggatacccc aaacggttgg tttcattagc gcaatgtagg ctatctttgc gaattgtatg
     1801 ataagagtca cactgcttct cctgctgtcc tccatcggtg ttaacgtcag cgcgcagacg
     1861 cattttaccg tcagcggcac catcagggac aaaaagaccg gggagatact gatcggggcc
     1921 tcggtcagtc ttttggaaat cccccggtcc ggcatcgcca gcaatgccta cggcttttat
     1981 tccatcagcg ccccgccggg gaaatatacg ctccttgtca gcttttcggg ctatgagccg
     2041 gacagcattc cggtccggct ggatcaaaac atcggtctcc ccgtggacct cgcgacggca
     2101 ggcggcgcgc tcacggaagt cgtcgtcagc gcacagaaaa agaacgagaa cgtcaccagg
     2161 gcattgatgg gtgttcagaa actgaccacc cgcgagatcc ggaatgtccc ggtacttttc
     2221 ggagaaaagg atgtcctcaa gaccatacag ctgctgcccg gcatccagtt tgcgggcgac
     2281 ggcaacagcg ggttctatgt ccgcgggggc ggtctggatc agaacctcat cctgctggat
     2341 gaagccacgg tttacaaccc ttcgcatctt ctcggttttt tttccacctt caattcggac
     2401 gccatcaagg atatcacggt ctacaagggc ggcatgccgg cggagtatgg gggacgtctg
     2461 tcgtccgtcg tggatatcaa aatgaatgac ggcaataaca aagagtatca tttcggcgga
     2521 ggaatagggc tcatctcgac acgtctcaat gccgagggac ctatcgtaaa ggaccgcggc
     2581 tcgttctcca tcagcggcag acgcacctat gcagacgtgt tcctcaaaat atccccggat
     2641 tcttcggtca accagaacag cgtttatttt tacgatctta acatcaaggc caattaccgt
     2701 ttcgacgaaa acaaccgggt ctatctctcc ggctatttcg gccgcgacaa tctctccttc
     2761 gccaacacct tcggcatcga ttatgggaat gccaccggca ccgcccgctg gaaccatgtc
     2821 ttcaacagcc ggcttttctc caatacatct ctcatctata gcaaatacag ctacaagatc
     2881 aagatcaatt cggccaataa cgatatcggg ttcacctcga atatccgcga tgtcaatctg
     2941 aaggaggacc tgcaatactt cgtcgacgcc ggcaataaga tcgatttcgg cttcagcgtg
     3001 atccatcatg cgatggcgcc gggtatcatc tccgcgtccc cggggtccag cttcaacccg
     3061 ctgacccttc aaaacaaata cagcctggag aatgccctct atatctcgca cgaatggtct
     3121 ccttcggaca gatggcatct gaattacgga ctcagagcct cctatttcct cgtactcggg
     3181 ccgggcaaat tctataccta tgactcatcc ggcaatacga aggattccac gcaatacaac
     3241 tccggcagca tcgtgaccac gtataccaat cccgaacccc gggcgtcgct gagttttcaa
     3301 atcaaccaga ccagctcgct caagatatcg tataaccgta acagccaggt gctgcacctg
     3361 ttgtccaatt ctacctcatc gaaccctacc gatctctgga tacccagcag caacaacgtg
     3421 aaacccgaga tcgccgacca ggagtcgatc ggctatttcc gtaatctccg tgacgatcgt
     3481 tatgaatttt ctgtcgaagc ctattataaa tcgatgcaga accagatcga ttaccgcaac
     3541 ggggcagagc tcatcgccaa tgagaatgtc gagtcgcagt tgttgttcgg aaagggcagg
     3601 gcatatggcc tggagctttt cctgaagaag aaagcgggga agttcaccgg gtggatcagc
     3661 tatacccttt cgcgcaccga gctgaagatc gacggtatca atcggaacaa ctggtatccc
     3721 gccaaacagg accggacaca tgatgtctcg ctggtcggca tttaccaggc cagccccaaa
     3781 tggacgctgt cggccacctg ggtatattat accggtagcg ccgtcacctt cccgaatggc
     3841 aaataccagg tgggcgggat gaccgccttt tattataccg aacgaaatgc ctatcgcatg
     3901 ccggcctacc accggatgga tgtcgcagcc acattgcagg ggaaaaagaa aaagaaattc
     3961 gaaagcagct ggacgttttc cgtgtacaat ttgtatgggc gggaaaatgc ctacagcatc
     4021 atttttcaga acgatcccga cgatccgtcg aagacccagg ccgtgcagta tgcgcttttc
     4081 cgttttgtac cgtccgtaac ctataatttt aaattctgac cgatgaaacg accaaccctc
     4141 ctccttttgg ccatatgcgc cctcgcgata gcggcatgca aaaaggttat ccaggtggat
     4201 ctcaacagcg tcgcacccca gatcgtcatc gaaggcgagg tgaccaatgg tccggctcca
     4261 tattttgtga ggatcagtaa aaccgtggat ttttcggcaa gcaatgatta tccgccggtg
     4321 acggatgccg tcgtcctgct gacggacact accaacggga tcagcgattc gatggtggag
     4381 gcgagcccgg ggctgtacaa aagtatcgag gtgggaggag tgccgcatca tacctatcgg
     4441 ctcgatgtga cgattgaagg aaagcagtat acggccttat cgaccatgcc cgagcgcgtc
     4501 aaactggatt cggtgacctt cgcgcaaaac atcgggttcg acaataaatt agagatcaat
     4561 gccgtggcag gattccagga cccgcccggt attgccgatt attaccagct ctccgagacc
     4621 gtcaacagcc ggctgatacc cgatattttc gttttggagg atcgactctc ggacggcagg
     4681 tatatccggt atccgctgta taatgacagc gcctattttg ctccgggaga tacactcagg
     4741 ctgacgatga attgcatcga caggaacatt tacaactatt tctttcaatt gatgaatgta
     4801 gccggcaaca acaacttcca gtcggcgacg ccgactaacc cggcgaccaa tatcaagggc
     4861 ggcgcgctgg gttatttcag cgcacacacg acggagaagg tcgacattat cgtgtactaa
     4921 ggcggactgg atggcggtgg gggcggctaa ggctgatagc ttttgatctt attgtacagt
     4981 gttttccgat cgatcttgag gatctcggcc gccctcgatt tgttgtaatt cacctgtttg
     5041 agcacattca tgatcgtgtc gtattcggcc tgtgcggcgg catttttcag atcggtgtcc
     5101 ttgtgcgcga tgggatgatg cggcgccgcc acgatgggaa catccaattg ctgcggtata
     5161 aagttctgcg aataggtgtt ggtatctacg atctcagcgg gcagcacccg cgcattgaca
     5221 cggcccgaag aggtaagtaa cgccgcgcgc cggatgacat tcctgaactc gcgaagatta
     5281 cccggccagg gataattcat gaatatctgc atgacctcgg gctcgaaacc ttcgacttcc
     5341 ttattcagct ccttattcgc cttcgagagg aagaattcgg cgaagagagg gatgtcctct
     5401 ttgcggtgac ggaaggcagg caatacgatc gagaattcgt tgaaccgatg gaaaaggtct
     5461 tcccggaaac gtcctttccg ataggcttcc tgcagattct cattggaagc gacgatgatg
     5521 cgtacatcgg tttccatttc cttgacgcct ccgacacgtt tgaatttcct ttcctggatg
     5581 accctcaaaa gagcggcctg gatctcataa gaaagattcg ctacttcatc gaggaagagt
     5641 gtgccgccgt tggccagttc gaaatgacct tctttatcac ccaatgcgcc ggtaaaagct
     5701 cctttcacgt ggccgaacag ttcgctgccg gccagttctt tcgaaagcgt accgcaatcc
     5761 atggcgacaa agggtttgtc ctttcgcgcg ctgttctggt ggatcgtacg cgcaatgacc
     5821 tcttttccgg tgccgctttc cccatacagg atgatactat agtttgtggg tgcgaccaat
     5881 tcgatctgcc tgtacaactc tttggtggca ggcgactggc ccacgaggaa ttcatcatcg
     5941 gacggaatgt tcctgccagg cttgcgcatg gcttgcacgg ccgtcgtggg tttgggtgta
     6001 tcacctgctt cggattgctg cagcgcgcgg ccgatgacat tcagcacttc ttcaggtatc
     6061 agcggtttgg tgatataatc gaatgcgccc gccttgatca catccacggc ggtcttgata
     6121 tcggaatatc cggtaatgat gatcacaatg gcataaggat cgacgttctt gatctcccgc
     6181 aaaacttctc taccctcttt gtcacccaga cggaaatcac atagtacgac atcgaatttg
     6241 gattctttga atttttgtat gcccttgccg ccgctgtggc ttgcttccgt ctcaaatccc
     6301 tttttttgca ggaaacggcc aagcaaattg cacatatcca tatcgtcgtc tatgatcaaa
     6361 attcgcttca taatttgatt gaggtggttt tcgaactaaa attacaggaa aaagtataat
     6421 ttggaatcag tcaactattt acctctcatt ctatcctaaa c
//
LOCUS       DASYSV010000319         4613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_959899, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000319 DASYSV010000000
VERSION     DASYSV010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959899"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VGQ51_09520"
     CDS             complement(<1..208)
                     /locus_tag="VGQ51_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036850.1"
                     /translation="MNKRMLFLMVVVIFSFAPRIIYGQQYVFDKKIPVPGDGGYDYMA
                     IDEVNNHLFVSHGTSFNVIDLATEQ"
     gene            333..752
                     /locus_tag="VGQ51_09525"
     CDS             333..752
                     /locus_tag="VGQ51_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036851.1"
                     /translation="MKVLFVIPLLISVANAQDIKTKDVPPAVRNTLMQKYPEATRVSW
                     EKEKGNFEANWGGKSGEDHSVQFRPDGSFVEAVDAMRVKELPAVVGQYVKEHYHTTIR
                     EAGKRLDARGTHTLEVEIRGKDLLFTPEGKFLSEEHG"
     gene            complement(810..3155)
                     /locus_tag="VGQ51_09530"
     CDS             complement(810..3155)
                     /locus_tag="VGQ51_09530"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEV9036852.1"
                     /translation="MRIACTLTIALFLLAGTAANGQGHHAKKEEHKSHIEYSLDTVMQ
                     KIDDLYETLNNINIFQQKHFDTANIRHQLSGINTTLDRIRDNLVSNAVTEYKRVLLDE
                     YVLKDIRNQLQLWRNELFQYNNNLVKMNAEIAAFTKDSVLQQLITDSIYREMYLDELA
                     QLRQKWRAARKNTDTSLVRVNALQSAISKPWFATLDLIAQVTIIKGQIAGVLFKKEYP
                     YIWEGDIASGDQFSNLTARSFRSESGLADYFWTRNWGYYLYALVIGLLFFYWVWNNFR
                     RVRTEEKGPEALQELHLYYLRPLPVLSALVIILTILPFFNFNAPTSFTQLGQLVLVIV
                     VSILFWLQWPRRLFRYWIYIVVFYILFVGTGAALVPKLGARIWLLALSVGSIFLVRNS
                     LRQIRGMFPYPRVMRIAWWIFLLLDMLSVLSNILGRLSLSKIFVTTAIFSLVQIILLS
                     VFVDCIVEALRLQTVESRLRQQKEQARATNQAALFGKMETSVYRLLALIAFLTWAMAF
                     TINLNIYDPAYALAVRIWDHTVKLGSVSFRVGNVLLFIFVLYVSNQLQKYIGFLYGTS
                     DGGQAQPGHKGSRLVMFRLILIIAGFMLAIVASGLPIDRITIVLGALGVGIGLGLQNI
                     VNNLVSGIILIFERPFQIGDYIELNGKKGVVRDIGIRSSKMMTENGTEIIMPNGDLLS
                     GEVINWTIRNNQIRIEIPMTVEGGHPFEEISGIITEALKEHSDLSKDDQPRVLLNSAN
                     EKATSFTVLLYVGNIGQIQTIKSEIIRLLIGKLAEHQIKTV"
     gene            complement(3229..>4613)
                     /locus_tag="VGQ51_09535"
     CDS             complement(3229..>4613)
                     /locus_tag="VGQ51_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9036853.1"
                     /translation="LLSLFSLLLALGVAAALLPLFNDLAGKQLHATTLFSGRLLPVLI
                     ALVVIVGCLAGSYPAFYLSSFQPIHVLKGKIAAGFRSSWLRSSLVVFQFFISIGLIVS
                     TIVIYRQLSYIRSRSIGFNRDHVLIIHHAYLMGDPVKTFRGDISRLSGVTDATLSGDL
                     PTDGGGYNQNGWFRDASLDAKRVIVLTSLSADQHYIPTLDMRMAKGRNFAPDTYPTDS
                     TGIILNEAAVQLLGLKQPLGALLYRPTDSLKGSIALHVIGVVKDFNFSSMHQKVGPLV
                     ISLGDNRGSMAIRLRAGDPSGIVRQVETKWASMAQGVPFNYTFMDNDFNNLYHAEQQT
                     GQVFITFAVFAILIACLGLFGLVTYAAEQRVKEIGIRKVLGARVGGIVTLLSKDFARL
                     VLIASLIAFPVAWWAMEKWLQSFAYRVGISWWIFVVAGLLAILIALVTVSFQTIRAAV
                     ANPVRSLRSE"
BASE COUNT         1097 a         1174 c         1340 g         1002 t
ORIGIN      
        1 gctgttcggt agccagatcg ataacattga aagaggtgcc atgcgagacg aagagatggt
       61 tattcacctc gtcgatcgcc atataatcat aaccgccgtc gcccgggacg ggtatttttt
      121 tatcaaaaac atattgctga ccataaatga tccggggggc gaaggaaaaa atcaccacca
      181 ccatcaggaa tagcattctt ttattcatag ctggtattgt tagacgcaca aactcgggtc
      241 caaacctgta gataaactgg cggcacccct accttcagaa agttctcagt atcgacacag
      301 tatgtcgtgt agctttacaa aaactatcga ccatgaaagt actattcgtc attccattat
      361 tgatcagcgt tgctaatgcc caggacataa agacaaaaga tgttcctcct gccgtcagga
      421 atacgctgat gcaaaaatat cctgaggcca cgagggtcag ctgggaaaag gaaaagggaa
      481 atttcgaagc caactggggg ggaaagtcag gcgaggatca ttcggtgcaa ttcagaccgg
      541 acggcagctt cgtagaggct gtggatgcca tgcgggtgaa agagttgccg gcggtggtag
      601 gccaatatgt gaaggagcat taccatacga cgatccgcga agccggaaaa cgtctcgacg
      661 ccaggggaac gcatacgctg gaggtggaga tcaggggtaa ggatttgttg tttacacccg
      721 aagggaaatt tctttcggag gaacatggat agcgagcctt ccgcgccttc cgcgccttcc
      781 gcgccatcta gcgtcgcggc gccgcgccat cacaccgtct ttatctggtg ctccgccagc
      841 ttcccgatca gcagccgtat gatctcgctc ttgatcgttt gtatctgacc gatattgccg
      901 acgtagagga gtacggtgaa gctggtggcc ttctcgttgg cggaattgag cagcaccctt
      961 ggctgatcat ccttgcttag atcggaatgc tctttcagcg cttccgtaat gatgccggat
     1021 atctcctcga agggatggcc cccttccacc gtcatgggta tctcgatcct tatctggttg
     1081 ttgcggatcg tccagttgat gacctctccg ctgagaaggt cgccattggg cattatgatc
     1141 tcggtgccgt tctcggtcat cattttgctg gaccggatac cgatgtcgcg aacgacgcct
     1201 ttctttccgt tgagctcgat ataatccccg atctggaagg gccgctcgaa gataaggatg
     1261 atgcccgaaa cgaggttgtt gacgatgttc tgcaacccga gaccgatgcc tacgccgagg
     1321 gcgcccagca cgatggtaat gcgatcgatc ggcaagccgg atgcgactat cgcaagcatg
     1381 aagccggcga tgatgagaat aagccggaac attacgagac gggatccttt atgacccggt
     1441 tgagcctgcc cgccgtcgct ggtaccgtag aggaagccga tatatttctg cagctggttg
     1501 gacacgtaaa gcacgaatat gaacagaagc acattgccga cacggaagga aaccgaaccc
     1561 agctttacgg tatgatccca tatccgtacc gccaatgcat atgccggatc gtagatattc
     1621 aaattgatcg tgaaggccat cgcccaggtg agaaaagcga tcaacgccaa aagcctgtag
     1681 acgctggttt ccatcttgcc gaacaaggcg gcctgattgg tagccctggc ctgttccttt
     1741 tgctgacgca gccggctttc cacggtctgg agccgtaagg cctcgacaat gcaatccacg
     1801 aagacggaga gcagaatgat ctgcacgagg ctgaagatcg cagtagtgac gaatatcttc
     1861 gagagactga gtcggccgag tatattgctg aggacggaga gcatatccaa tagcaggaat
     1921 atccaccagg cgatgcgcat cacacgggga taaggaaaca tgccgcgtat ttggcgaagc
     1981 gaattcctga ccaggaagat cgagccgacg ctgagggcca gcagccatat cctggcgccc
     2041 aatttcggga cgagcgccgc cccggtgccg acgaacagga tatagaacac cacgatatag
     2101 atccagtacc ggaacaatcg gcgcggccat tgcagccaga aaagtatact cactacgatc
     2161 acaaggacca actgtcccag ctgtgtaaag gaggtaggcg cattgaaatt gaagaacgga
     2221 aggatcgtaa ggatgataac gagggcggac agcacgggca ggggccggag ataatagaga
     2281 tgcagctcct gtaaggcttc cggacctttc tcctccgtcc tgacacgtcg gaaattgttc
     2341 catacccaat aaaagaacag aagtccgatg acgagggcgt ataaatagta tccccagttg
     2401 cgcgtccaga aataatccgc gagaccactt tcggagcgga aggatcgggc ggtcaggttg
     2461 ctgaattgat cgcccgaagc gatgtcccct tcccagatat agggatattc ctttttaaaa
     2521 agtacgccgg cgatctggcc tttgatgatc gtgacctgcg cgatcagatc gagggtcgcg
     2581 aaccagggtt tggaaatggc cgattgaagt gcgttcacgc gtaccaggct ggtgtcggta
     2641 tttttgcggg cggccctcca tttttggcgc aattgcgcca gttcatcgag gtacatttcg
     2701 cggtaaatgc tgtccgtgat cagctgctgg agaaccgagt cttttgtaaa ggcggcaatc
     2761 tccgcgttca tcttcaccag gttgttattg tactggaaca gctcgtttcg ccagagctgc
     2821 agctggttgc ggatatcttt cagcacgtat tcatccagga gtaccctttt gtattcggtg
     2881 acggcattgg agacgagatt atcgcggatc cgatccaggg tggtgttaat gcccgacagc
     2941 tggtggcgga tgttggcggt atcgaaatgt ttttgctgaa agatgttgat gttgttgagc
     3001 gtctcgtaga gatcgtcgat tttttgcatc accgtgtcga gtgagtactc gatatgcgat
     3061 ttatgctctt cctttttcgc atgatgaccc tggccgttcg cggcggttcc ggccaggagg
     3121 aatagggcaa tggtaagggt gcatgcgatt ctcatcccgg ggtactatgc aatggggatg
     3181 ccagcggccc ttcgcgatca ttcggaagct tgtgccggcc cttcgcgatc attccgaccg
     3241 gaggcttcgg acggggttag caacggcggc gcggatggtc tgaaagctaa cggtcaccag
     3301 cgcgatcagg atcgccagta agccggcgac cacgaagatc caccagctga tgccgacacg
     3361 ataggcaaag ctttgcagcc atttttccat ggcccaccag gccacgggga aggcgatcag
     3421 cgatgcgatg aggacaagcc gggcgaaatc tttgctcaaa agcgtcacga tcccgcccac
     3481 gcgggctccc agcactttac ggatacctat ttcttttacg cgttgttcgg ctgcataggt
     3541 caccagaccg aagaggccca gacaggcgat gaggatagcg aaaacggcaa aagtgatgaa
     3601 gacctgtccc gtctgctgtt cggcatgata caggttgttg aaatcattgt ccatgaaggt
     3661 atagttgaac ggcacgccct gcgccatgct cgcccatttg gtctccacct ggcggacgat
     3721 gcccgaaggg tcgccggcgc gtaaacgaat ggccatactg ccccggttgt cgcccaggct
     3781 aatgaccagg ggtcccactt tctggtgcat gctgctaaag ttaaaatcct ttaccacgcc
     3841 gatgacatgc aaagcaatac tgcctttgag gctatccgtg ggccggtaga gtagggcgcc
     3901 aaggggttgt ttcaatccca gcagttgtac ggcggcttca ttgaggatga tgccggtgga
     3961 gtcggtcgga taggtgtccg gcgcgaaatt cctgcccttc gccattcgca tgtccagcgt
     4021 ggggatgtag tgttgatccg ccgaaaggct ggtcaggacg atcacgcgtt tggcgtccag
     4081 cgaggcgtcg cggaaccagc cattctgatt atagccgccc ccgtcggtgg gcagatctcc
     4141 gctaagcgtc gcgtcggtga cgccggacaa gcgggatata tctccgcgga atgtttttac
     4201 cgggtcgccc atcaaatagg catggtgtat gatgagcaca tggtcgcggt tgaaaccgat
     4261 ggacctgctg cggatatagc tgagctggcg atagatgacg atcgtgctga cgatgagtcc
     4321 gatggagatg aagaattgga aaacgaccag gctgctgcgg agccagctgc ttcggaagcc
     4381 ggctgcgatc tttcctttaa gcacgtgtat cggctggaag gacgagagat aaaaagcagg
     4441 gtaactgccg gccaggcagc ccacgatgac caccagcgct atcagcaccg ggagcaggcg
     4501 cccggagaac agggtggtcg catggagctg cttacctgcc agatcgttga acaggggcag
     4561 caaggcggcg gcgacaccca gcgccagcag cagcgagaac aggctcaaca gga
//
LOCUS       DASYSV010000320         6769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2493364, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000320 DASYSV010000000
VERSION     DASYSV010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493364"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VGQ51_09540"
     CDS             complement(<1..1157)
                     /locus_tag="VGQ51_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEV9036854.1"
                     /translation="MRIFPFRWFKNVSIAKKLYFTVGIMALLIVIELMVLFFSINTLS
                     SVRAYVGGEGLWSKAQKDALYQLLKYARTRDELDYVKFENFMKVNEGDHMARIELSNP
                     SPDFDLVKKGFIEGRNDPGDVAGMIHLFRWFHSNAYIQRAIVAWTNADSVLSDFMTLG
                     RRLHTVINTAPYSQEHVNAILDEIDPLNDRLTPLEDEFSFALGEGSRWLERVILRLLF
                     FVALSVECTGLLLAITVSRGIQIGLQEILLSARSVTRGDFSRKAKAYSTDEIGTLAEN
                     FNSMAAELQRLEKENREVNSSLERKVRQRTSEMESKNKELEQFAYVASHDLQEPLRTI
                     SGFVDLLQKDYGDRLDDNGHIYLNYLSQASDRMKTLIKDLLDYSRLGREKEA"
     gene            complement(1286..1468)
                     /locus_tag="VGQ51_09545"
     CDS             complement(1286..1468)
                     /locus_tag="VGQ51_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036855.1"
                     /translation="MKYVITLLLLIGVVINGLTGYRFFRTASYDLSSLLIVASYFSIV
                     FSIYVLTMGKRKTAFE"
     gene            1548..2546
                     /locus_tag="VGQ51_09550"
     CDS             1548..2546
                     /locus_tag="VGQ51_09550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEV9036856.1"
                     /translation="MSGTKDKVLVTRPLFPDVVDRLREYFEVMVNEGPRYTSEQLHDA
                     LSGMKGAIVAGGEKIDDRVLQGHTGLKAICVSAAGFNNVDVAALTRAGIIGTNSPGPA
                     DETVADFTWGLMIATARRLVEAAFWVERGEWKATAMASRFFGSNLCDKTLGILGMGRI
                     GQAIARRAVGFRMNVLYNNRRRLDTGLEVECRAAYRTREDVLREADLLVLAMPYSPEN
                     HHLIGAAELRLMKPTAMLFNIARGGLIDETALAAALRSGALAGAGLDVFEAEPVIEPG
                     LRDLPNVILTPHTAGATEEAQHGLASMAADNLIAALGFGPDAGRPCGVFNPEVLGK"
     gene            complement(2673..3998)
                     /gene="ahcY"
                     /locus_tag="VGQ51_09555"
     CDS             complement(2673..3998)
                     /gene="ahcY"
                     /locus_tag="VGQ51_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEV9036857.1"
                     /translation="MSTKTQSIDLKLPYKVKDMSLAEWGRKEIRLAEAEMPGLMAIRA
                     EYGKQKPLKGARIAGCLHMTIQTAVLIETLVELGAEVRWSSCNIFSTQDQAAAAIAAA
                     GIGVFAWKGQTLEEADWCIEQTLFFGSTDRPLNMILDDGGDLTNMVFDKYPELVQHIK
                     GLSEETTTGVHRLYERMEKGTLPIPSINVNDSVTKSKFDNKYGCKESLVDAIRRATDV
                     MMAGKVAVVGSYGDVGKGSAASLKGAGCRVIVTEIDPICALQAAMDGFEVKKMVDAVK
                     EADIVVTASGCRDLITEKHFRLMKDKAIVCNIGHFDIEIDMAWLNKNYGHTKDTIKPQ
                     VDVYNIDGRDVIILAEGRLVNLGCATGHPSFVMSNSFSNQTLAQIELWTNHSKYENKV
                     YVLPKHLDEKVARLHLAKIGVQLDELTDAQAQYLGIPKAGPFKPEHYRY"
     gene            4135..4641
                     /locus_tag="VGQ51_09560"
     CDS             4135..4641
                     /locus_tag="VGQ51_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEV9036858.1"
                     /translation="MEKDGQKEKPATANNPVELDEKDRAILRLLQANAKITVREIAAQ
                     VHLSTTPVHERIRRMEETGIIRQYATLLDHSKVRKGLLAICYVSLKEHNKRSGARFIR
                     TMNELSEVTECYIISGEFDFMLKIAVENMDAYYDFHVNKLGQLENIGHVQSTFVMGVV
                     KQTHQLVD"
     gene            4693..4995
                     /locus_tag="VGQ51_09565"
     CDS             4693..4995
                     /locus_tag="VGQ51_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036859.1"
                     /translation="MYVYDTVAEAVKGLKQRGYNIDFNIVADKLHCAGPSLALRPDDF
                     RITEFYRFEGESDPADEAVVYAIESTGGEKGLLVTGFGITAEGFGEEMIEKLRLKR"
     gene            4992..5510
                     /locus_tag="VGQ51_09570"
     CDS             4992..5510
                     /locus_tag="VGQ51_09570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9036860.1"
                     /translation="MISIRQATERDLPSLLDIYNHVILHTTAVYSYQPHTMEARREWY
                     SSKIKDGYPVYVAEEDGRVIGFSSYGPFRAWPAYKYTIENSVYVAEDQRGKGIGKLLI
                     RPLIDEARRQGYHAIIASIDASNISSLRLHGSFGFEEVAHFRQVGFKFGRWLDLKFLE
                     LLLPTPEDPTED"
     gene            complement(5497..5958)
                     /locus_tag="VGQ51_09575"
     CDS             complement(5497..5958)
                     /locus_tag="VGQ51_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV9036861.1"
                     /translation="MHNVRVYGILMGPDKQVLVSDEYIRGGYYTKFPGGGLEFGEGTR
                     DCLKREFKEEMDLDIAVTDHLYTTDFFQMSAFNSTQQIISIYYFVQALEPIRVPLRMK
                     PFDFDEQQLKIYEERKEIETFRFIDWEDLSEESVNLPIEKIVVRLLKTNLR"
     gene            complement(5962..>6769)
                     /locus_tag="VGQ51_09580"
     CDS             complement(5962..>6769)
                     /locus_tag="VGQ51_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV9036862.1"
                     /translation="PLDYLTDSLDIDERARFEEYDNVKLIVIKTPTENNSFNESDAYY
                     ITIPICIILTHNQIVTVNSFENGAIKKFLNTFQNRHHDKKNMMVLKVFEKITQTFMEY
                     LKEINQRRNLMEQKLYASNRNEELLELMKIQKSMVYFVTALRSNEMLLLKLARTNFLG
                     LNDEEKEFLDDLAVDMSQALEMANIYTNILSSTLDAFASIINNNMNNVLKRLTSITII
                     LSLPALVTGIYGMNVPIPYSDSHYAFYIPILLSVGISIIISWYFMKKKWF"
BASE COUNT         1529 a         1633 c         1953 g         1654 t
ORIGIN      
        1 gcctctttct cccggccgag acgtgagtaa tcgagcaggt ccttgatgag cgtcttcatc
       61 ctgtccgacg cctgcgaaag atagttcagg tagatatgcc cgttgtcatc gagccggtcg
      121 ccataatctt tctgaagaag atccacaaac ccggagatgg tacgcagggg ttcctgcagg
      181 tcgtgcgagg cgacgtaggc aaactgctcc agctccttat tcttgctttc catttccgac
      241 gtacgttgtc gcacctttct ttccagcgag ctattgacct ccctgttctc tttttcgagc
      301 cgttgaagct cggccgccat cgaattgaag ttctcggcca gggtgccgat ctcatcggtg
      361 gaataggctt ttgccttccg gctgaaatcc cctctagtca cacttcgggc ggacagcagt
      421 atctcctgta aacctatttg gataccgcgg ctgacggtga tggcgagcag caaccccgta
      481 cattccacgg atagcgccac gaagaaaagc agccgcagga tgaccctttc cagccagcgt
      541 gagccttcgc ccagcgcgaa cgagaactcg tcttccagcg gggtgagccg gtcattcaac
      601 ggatcgatct catccaaaat ggcgttcacg tgttcctgcg aataaggggc ggtgttgatc
      661 acggtgtgta accgtcgacc cagggtcata aaatcggaga ggaccgaatc ggcatttgtc
      721 cacgcgacga tggcccgttg gatataggcg ttggagtgaa accatctgaa cagatggatc
      781 attccggcga catcgccggg gtcgttccgt ccttctatga aacctttttt gaccagatcg
      841 aaatcgggac tgggattgct gagctcgatc cgggccatgt ggtcgccctc gttgaccttc
      901 atgaagtttt cgaacttgac ataatccagt tcgtcgcggg tccgcgcata tttgagcagc
      961 tggtagagcg cgtctttctg cgcctttgac cagaggcctt ctccgccgac ataggcgcgg
     1021 acggaagaaa gggtattaat ggaaaagaaa aggaccatca gctcgataac gatgaggagg
     1081 gccatgatcc cgaccgtaaa ataaagtttc ttggcgatgg agacgttctt gaaccatctg
     1141 aacgggaaga ttctcataat gtgggttttg gcgacggccg gagcgacaat gtaaggaagt
     1201 tgcggggaaa tgccaaaagc ggagcacggg cgcacaacaa aagaccccgg tcatctggcc
     1261 ggggtcttca ggtatgcgga tgcatttact cgaatgccgt tttccttttg cccatcgtca
     1321 gtacatagat gctgaaaacg atagaaaaat aactggcgac gatcaggagg cttgagagat
     1381 cataggacgc ggtacgaaaa aaacgataac cggtaagacc attgatcact acgccgataa
     1441 ggagcaagag tgttattacg tatttcatac atttacgttt tagtcaggag atgttgttta
     1501 atttatagac ccgcgattat acgggtagtt tattaatgaa atgttatatg agcggaacca
     1561 aagacaaagt ccttgtaacc aggcctctat ttcccgatgt ggtggaccgt ctccgggaat
     1621 atttcgaggt gatggtaaac gaagggcctc gttatacgtc ggaacagttg catgacgcgt
     1681 tgagcgggat gaagggcgct atcgtggcgg ggggcgaaaa gatcgatgac agggtcctgc
     1741 aggggcatac cggtctgaag gccatctgcg tgtctgcggc gggttttaac aacgtggatg
     1801 tagcggcact gacacgtgcg gggatcatcg gaacgaattc cccgggtccg gccgacgaga
     1861 ctgtggcgga tttcacctgg ggtctgatga tagccaccgc ccgccgtctg gtggaggcgg
     1921 cgttctgggt ggaacgcggc gagtggaagg caacggcgat ggcaagccgt tttttcggca
     1981 gcaatctctg cgacaagacg ctcggcatcc tcggtatggg tcgtatcggg caggccatcg
     2041 cccggagggc cgtcggtttt cgcatgaatg tgctgtataa taaccgtcgt cggctggata
     2101 ccggtttgga ggtggaatgc cgggctgctt atcgtacccg ggaagatgtt ttgcgcgagg
     2161 ccgatctctt agtcctggcc atgccgtact cgcccgaaaa tcatcatttg atcggagccg
     2221 cggagctgcg cctgatgaag ccgacggcca tgcttttcaa catcgcccgc ggcggtctga
     2281 tcgacgaaac ggcattggct gcggcgttgc ggagtggcgc gcttgccggc gcaggtctcg
     2341 acgtcttcga agcggagccg gtcatcgagc caggattgcg cgatcttccg aatgtcatcc
     2401 tcacaccgca taccgcaggg gccacggagg aggcacaaca tggattggcg agtatggctg
     2461 cggataacct gatcgctgcg ctgggtttcg ggcccgacgc agggcggcct tgtggtgttt
     2521 tcaatccgga ggtgttgggg aagtgaggtc tccggcggcg gatgtgaggt ttcctgcggc
     2581 tgtgatgcga ggaatgaaag gcccttaaaa gatacgaaaa agcccgcttc gaaagaagcg
     2641 ggctcttaat gttaacaggc gaaagcccgg gattagtagc gataatgttc aggtttgaac
     2701 ggcccggctt tgggaatgcc gagatattgg gcctgcgcgt ccgtcagctc gtcgagctgt
     2761 acgccgatct tcgccagatg cagacgggct accttttcgt cgagatgttt gggcagaacg
     2821 tataccttgt tctcgtattt gctgtggttg gtccacaact cgatctgcgc cagtgtctgg
     2881 ttggagaaag agttgctcat cacgaatgag ggatgtcccg tagcgcaacc caggttgacg
     2941 agtcgtcctt cggccagaat gatcacgtcg cggccgtcga tgttgtagac atcgacctgc
     3001 ggtttgatcg tatctttcgt atgaccgtaa ttcttgttca gccatgccat gtcgatctcg
     3061 atatcgaaat ggccgatgtt acagacgatg gccttgtctt tcatcaggcg gaaatgtttt
     3121 tcggtgatga ggtcgcggca acctgaagcc gtaacgacga tatcggcctc tttaacggca
     3181 tcgaccatct tcttcacttc gaaaccgtcc atggcggcct gcagggcaca gatgggatcg
     3241 atctcggtca ctataacgcg gcagcccgcg cctttcaggc tggcggcaga acccttgccg
     3301 acatcgccat agctgcctac gacggcgacc tttcctgcca tcatcacatc ggtagcgcgg
     3361 cggatggcgt ccaccagcga ttccttacag ccgtatttgt tatcaaattt ggatttggta
     3421 acggagtcgt tgacattgat ggagggaatc gggagagtac ctttttccat ccgctcgtac
     3481 agacgatgaa caccggtggt ggtttcttcg ctgagaccct tgatatgctg taccagctcg
     3541 ggatatttgt cgaaaaccat attggtgaga tcacctccgt catcgaggat catgttgagg
     3601 ggacggtcgg tgctcccaaa gaacagcgtc tgctcgatac accaatcggc ttcttccagc
     3661 gtctggccct tccaggcgaa tacgccgata cctgcggcgg ctatggcggc agcggcctgg
     3721 tcctgcgtcg agaagatgtt gcaggagctc caacgtacct cggcgcccag ttcgaccagg
     3781 gtctctatga gaacggcggt ctgtatcgtc atatggagac agccggcgat gcgtgctcct
     3841 ttgagcggct tttgtttgcc gtattcggca cggatagcca tcaggccggg catttcggct
     3901 tccgccaaac ggatctcttt gcggccccat tccgcgaggc tcatatcttt cactttataa
     3961 gggagcttca gatcgatgct ctgcgttttt gttgacattg ttattaggtt ttactgttat
     4021 tcggttttac gcgtcaaaga tatgatgata gaacaaaaat atatcaattg tgcacaattt
     4081 tggaacagaa ttgtactttt tgactatatt gtaggataaa acactacggc aaccatggaa
     4141 aaggacggtc aaaaagaaaa accggctacg gctaataacc cggtcgaact ggacgaaaag
     4201 gatcgggcga tcctccggct tttgcaggcg aacgccaaga tcacggtccg agagatcgca
     4261 gcccaggtgc atctcagcac gacgccggtg cacgagcgga tcaggcgtat ggaggaaacc
     4321 ggcattatcc gccaatatgc cacgctgctg gatcattcga aggtcagaaa ggggttgctg
     4381 gcaatttgct atgtttcgct gaaggagcac aataaacgat cgggagcgcg gttcatccgg
     4441 acaatgaacg agctcagcga ggtaacggaa tgctatataa tctcgggaga attcgatttc
     4501 atgctcaaga tagccgtcga gaacatggat gcgtattatg attttcatgt caataaactg
     4561 ggacagctgg aaaatatcgg gcatgtgcag agcaccttcg tcatgggggt cgtgaagcag
     4621 acgcatcagc tggtcgattg agtggcgggc tccgggccgg tgcggatcgg gcggatttcg
     4681 taaattgcag tcatgtatgt atatgatacg gtagctgagg ccgtgaaagg cctgaaacag
     4741 cggggataca atatcgactt caacatcgta gccgataagc tgcattgcgc cggtccgtct
     4801 ttggcgctga gaccggatga tttccggatc accgaattct atcgttttga aggagagagc
     4861 gatcctgccg atgaggccgt tgtttacgca atcgaatcga cgggcggaga aaaagggctt
     4921 ctcgttaccg ggttcggcat caccgccgag gggttcgggg aagaaatgat cgaaaaatta
     4981 agattgaaaa gatgatatcc atcagacagg caaccgaacg agacctgccc tcgcttctcg
     5041 atatttacaa ccatgttatc cttcacacga cggctgttta ttcctatcaa ccgcacacga
     5101 tggaagcccg cagggagtgg tattcctcca aaataaagga cggttaccct gtatacgtcg
     5161 ccgaggagga cgggcgggtc atagggttta gcagctatgg ccctttccgc gcctggccgg
     5221 cctacaagta tacgatcgag aactccgtct atgttgccga ggaccagcgg ggcaaaggca
     5281 tcggtaaact cctgatacgg cccctgatcg acgaagcccg ccggcaggga tatcatgcca
     5341 tcatagcctc gatcgatgca tccaatatat cgagcctccg tcttcatggc tcattcgggt
     5401 ttgaagaggt ggcgcatttc cggcaggtgg gatttaaatt cggccggtgg ctcgatctga
     5461 aattccttga attattgctg ccgacgccgg aggatcctac cgaagattag tcttaagaag
     5521 acggacgacg atcttttcga tgggaaggtt cacgctctct tcactgagat cctcccagtc
     5581 tataaagcgg aaggtttcga tctctttcct ttcttcgtat atctttaact gttgctcgtc
     5641 gaaatcgaag ggcttcattc gtagtggaac acggatcggt tccaatgcct gcacgaaata
     5701 atagatggaa atgatctgct gcgtcgaatt gaaggcggac atttgaaaga aatccgttgt
     5761 atagaggtga tcggtgacgg cgatgtcaag gtccatttcc tctttgaatt cgcgtttgag
     5821 acagtcccgc gtaccttcgc cgaattcgag cccgcctccg gggaatttgg tataatagcc
     5881 tccccggata tattcgtcgc tgacgaggac ctgtttatcg ggacccatca ggataccgta
     5941 tactctgacg ttgtgcataa gttagaacca ctttttcttc atgaaatacc agctgatgat
     6001 gatggatata ccgaccgaca gcaggatcgg gatataaaag gcatagtgcg aatcggaata
     6061 gggaatgggc acattcatcc cgtagatgcc ggtgaccagg gccggaaggg acagtatgat
     6121 cgtgatggac gtgagccgct tcagcacatt attcatgttg ttgttgataa tgctggcgaa
     6181 ggcatcgagc gtgctgctga ggatattggt atagatgttt gccatttcca gggcctgtga
     6241 catgtccacc gcgaggtcgt cgagaaattc cttttcttcg tcattaagac ccaggaagtt
     6301 ggttcgcgcc agtttgagca ggagcatttc attgctgcga agcgccgtga cgaaatagac
     6361 catgcttttc tggatcttca tgagctcgag cagctcttca ttccggttgg acgcgtagag
     6421 cttctgttcc atgaggttgc gccgctgatt gatctccttg aggtactcca taaaggtctg
     6481 ggtgatcttt tcgaagacct tcaggaccat catgttcttt ttgtcgtggt gtcggttctg
     6541 gaaggtgttg aggaattttt ttatggcgcc gttctcgaag gagttgaccg tgacgatctg
     6601 gttatgggta aggatgatac agatcgggat ggtgatgtag tacgcgtcgc tttcgttgaa
     6661 ggagttgttc tccgtcgggg ttttgatgac gatgagtttg acgttgtcgt attcttcgaa
     6721 acgggctctt tcgtcgatgt cgagggagtc ggtaagatag tccagaggg
//
LOCUS       DASYSV010000321         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_834155, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000321 DASYSV010000000
VERSION     DASYSV010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834155"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VGQ51_09585"
     CDS             <1..370
                     /locus_tag="VGQ51_09585"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV9036863.1"
                     /translation="VGMTRIHSVVYKDKWAEIGHTWYVPELWGQVYNKECKLLLLQYL
                     FETVGFYRVQFRIAHQNIRSQRAVEKIGGVLEGVLRKHAIRNDGSRRDTVVYSIIDEE
                     WPEKKARLQRMVAAAHTVAL"
     gene            complement(365..889)
                     /locus_tag="VGQ51_09590"
     CDS             complement(365..889)
                     /locus_tag="VGQ51_09590"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9036864.1"
                     /translation="MEDIVIHIRTAVANDAALIADFSRRTFFESFAQYNTAENMRLYL
                     DEQFPRERQMAEVGAPGRMFLLAYLGDRLVGFASLRESDPPEGLKGENGIEIVQLYSD
                     RETIGKGVGPALMQACLDTARSQGRDWVWLGVWENNHRAKAFYAKWGFERFGEHIFIV
                     GLDAQTDWWMRRRL"
     gene            complement(893..>2717)
                     /locus_tag="VGQ51_09595"
     CDS             complement(893..>2717)
                     /locus_tag="VGQ51_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9036865.1"
                     /translation="RTHEEHPAELSTTLVQKAIHYLFPAMLGSSISTIVIFIPFVLMS
                     GVAGAYFKVLTNTMIITLVCSFLVTWIGLPVIYLLLTRQRNKTRTLKPHNVKTQRWVA
                     FFIRRAWISLLIMGVLAGLIWWILPQLETGFLPEMDEGTIVLDYTSPPGTSLQETDRL
                     CREMEKIIVTTPEVEAYSRRTGTQMGFFITEPNTGDYEIQLKKDREKTTDEVIGYLRK
                     RIEATQPALRIDFGEVIGDMLGDLMTSVQPIEVKIFGNDQSELHHLSQQVADVVGKVE
                     GTADVFNGIVIAGPSVSIEPDYARLAQFGLTPANLQYQLQSSLEGNIVGTLPEKEQLT
                     NIRMVYPGSRTLSVADISRLQIFLPDGKLKPITDLARVTVKAGDAEIQRENLQSMGVV
                     TGRLEGRDLGGVMKDIQQKVSSGIALPAGYHITYGGAYAEQQQSFSELLVILITASLL
                     VFGVILFLYRRFAVALLILVIAVLGISGSYLGLYLTHTPLNVGSYTGLIMIVGIIGEN
                     AIFTFLQFRDSLATQTVDEAVIYAISTRLRPKLMTALGAIIALMPLALGIGAGAQLHQ
                     PLAIAVIGGFVIALPLLLIVLPSMLRWMYRGKGAASVMADI"
BASE COUNT          608 a          779 c          753 g          577 t
ORIGIN      
        1 cgtcgggatg acccggatac acagtgtcgt gtataaagat aaatgggcgg agatcgggca
       61 cacgtggtat gtcccggagt tatggggaca ggtctacaat aaggagtgta agctgttgct
      121 gttgcaatat cttttcgaaa ccgtgggctt ctatcgcgtc cagttccgga tcgcgcatca
      181 gaacatccgg tcgcagcgcg ccgtcgaaaa gataggcggg gtcctggaag gtgtgttgcg
      241 caaacatgcc atccgtaatg acggttcgcg gcgcgataca gtagtataca gtatcatcga
      301 tgaagaatgg ccggagaaaa aggccaggct gcaacgaatg gttgcggcgg cacacacggt
      361 ggctttatag acgccgccgc atccaccagt cggtctgcgc atccaacccg actataaaga
      421 tgtgctcgcc aaatcgctcg aatccccatt tcgcataaaa ggccttcgcc cggtgattgt
      481 tctcccagac acccagccat acccaatcac ggccctggga acgggcggta tccaggcagg
      541 cctgcataag cgccggccct actcctttgc cgatcgtctc ccggtcggaa tatagttgca
      601 cgatctcgat accattctcc cctttcaatc cttcgggagg atcagattcg cgcaacgagg
      661 caaagcctac cagccggtcg cccaggtatg ccagcaggaa catgcggccc ggcgccccca
      721 cctcggccat ctgtctttcg cgtggaaact gttcatcaag atagagacgc atattctccg
      781 cggtgttata ctgcgcaaaa ctttcgaaga acgtccggcg gctgaaatcg gcgatcaaag
      841 cggcatcatt tgccaccgcc gttcgaatat ggataacgat gtcttccata gatcaaatat
      901 ccgccatgac cgaggcagcg cccttcccgc gatacatcca ccgcagcatg gacggcagca
      961 cgatcagtaa cagcggcagg gcgatcacga aaccgccgat aacggcaatg gccaatggct
     1021 ggtgaagttg tgcgccggcg ccgataccca gcgccagcgg catcagggcg atgatggctc
     1081 ccagcgctgt catgagcttg ggtcgcagcc gcgtcgagat ggcatagatc accgcctcat
     1141 cgaccgtttg cgtagccagg ctgtccctga actgcaggaa tgtaaagatg gcattctctc
     1201 cgatgatccc gacgatcatg atcagaccgg tatagctgcc tacattcaat ggcgtatggg
     1261 taagatatag tccgagatag ctgcccgata tgccgagcac tgcgatcacg aggatcagca
     1321 aggctacggc aaaacgccgg taaaggaaaa ggataacgcc gaagacaagc aggctggcgg
     1381 taataaggat gaccagcagc tcgctgaacg actgttgctg ttccgcatag gcgccaccgt
     1441 aagtgatatg gtatcccgcc ggaagggcga tccccgagct caccttctgc tgaatatcct
     1501 tcatgacgcc gcccaggtcc cttccttcca ggcgtccggt gactaccccc atggattgca
     1561 ggttctcgcg ctggatctcg gcgtctccgg cctttacggt caccctcgcc agatcggtga
     1621 tgggtttcag cttgccgtcc ggcaggaaga tctgcaggcg gctgatgtcg gccacgctga
     1681 gcgtccggct tcccgggtaa accatgcgga tattcgtcaa ttgttccttt tcgggcagcg
     1741 tacctactat attgccttcg agcgagcttt gcagttggta ttgcaggttc gccggggtta
     1801 atccaaactg tgccagccgg gcatagtccg gctcgatgct cacggaaggg ccggcgatca
     1861 cgatgccgtt gaacacgtcc gctgtgccct ccaccttacc gacgacatcg gccacctgtt
     1921 gggacagatg atgcagctcg gactgatcgt tcccgaagat cttgacctcg atgggctgca
     1981 ccgatgtcat aagatcaccc agcatgtcac cgatcacctc tccgaaatct atccggagcg
     2041 cgggttgtgt cgcctcgatc cgtttgcgaa ggtacccgat cacttcatcg gtggtctttt
     2101 ccctgtcctt tttcagctgt atctcgtaat cgcccgtatt gggttcggtg ataaagaaac
     2161 ccatttgcgt tcccgtacgg cgggaatagg cctccacttc cggcgtcgtc acgatgatct
     2221 tctccatttc gcggcaaaga cgatctgttt cctgcagcga agtgccgggc ggcgaggtat
     2281 agtccagcac gatggtgcct tcatccattt caggcaggaa acccgtttcg agctggggca
     2341 atatccacca gataagccct gccagaacgc ccatgatgag caggctgatc caggcgcgcc
     2401 ggatgaaaaa ggcaacccac cgctgcgttt tgacattatg cggtttgagc gtccgcgtct
     2461 tattccgctg acgtgtcagc agcaagtaga tgaccggcag cccgatccag gtcactagaa
     2521 aggagcatac gagggtgatg atcatcgtat tggtcagcac tttgaaataa gcgcccgcta
     2581 ccccgctcat gagcacaaac ggaataaaga tcacgatcgt gctgatagaa gaacccagca
     2641 tggccgggaa caggtagtga attgctttct gaaccagggt cgtggacaat tcggcaggat
     2701 gctcttcatg cgtacgg
//
LOCUS       DASYSV010000322         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2493550, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000322 DASYSV010000000
VERSION     DASYSV010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493550"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VGQ51_09600"
     CDS             complement(<1..293)
                     /locus_tag="VGQ51_09600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9036866.1"
                     /translation="MIWLLVLPVGLIFLVYAGLILAYHRAWKAIPVFVRQAATAQATI
                     TVLIPARNEEANIAGCLGSLARQSYPRDRFEVIVLDDHSTDGTAETIRSFSGGG"
     gene            complement(299..1123)
                     /locus_tag="VGQ51_09605"
     CDS             complement(299..1123)
                     /locus_tag="VGQ51_09605"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV9036867.1"
                     /translation="MSEHFVSFRSSRFHYSRWGTGRRLLFAFHGYGESAASFGFLSAA
                     LAQDFTMIAIDMPFHGETEWKEEILFAPPDLLSLLVDIASELAGGNEEWTLIGYSMGG
                     RVALQLAELAPEKIRWLVLLAPDGLRVNPWYWLSTQTRPGNGLFSYTMRHPGWFFFLL
                     RAANYLRMVNPSVYKFTLHYIDDAEARRLLYTRWTVMSGFNPRPGPVSDIVRRRRIPV
                     HLLYGRFDRIIPSQRGQTFQRRCAPYCQLTVLPAGHQLLQSKFMEIIVSALYLPII"
     gene            1258..1698
                     /locus_tag="VGQ51_09610"
     CDS             1258..1698
                     /locus_tag="VGQ51_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV9036868.1"
                     /translation="MPNNQFRRGELYSFITGKASTAIARNLQKRFNAAGLKITIEQWS
                     VLYHLWKEDGKSQQELCSATFRDKPSITRLVDNLERQQLVKRVPSQDDRRINLIYLTR
                     QALRLQEETMQLAEATLNEALEGVPADRIEICKEVLQVVYDNLR"
     gene            1725..3518
                     /locus_tag="VGQ51_09615"
     CDS             1725..3518
                     /locus_tag="VGQ51_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV9036869.1"
                     /translation="MSTSTGKTQALKGAEWLIRESSPFDTFIPEDYNEEQRMVKDMCN
                     AFLDAEVLPIIDRIDKLEPGLMPSLIARAGEQGLLGASIPEEYGGLGKDFITSTLVNE
                     GLGGGFSFSVGVSAHTGIGTLPILYFGTPEQKQKYIPRLASGEWKGSYGLTEPNSGSD
                     ALGAKTTAKLTSDGKYYLLNGQKCWITNGGFADVYTVFAKIDGDKFSAFIVERGFEGF
                     TQGPEEHKMGIKGSSTVQLYFQDCKVPVENLLGEIGKGHIIAFNILNIGRLKLCAAAL
                     GGAKRATTISIEYANTREQFKTPISRFGAIQHKLAAMAIRMWTTESALYRTAKLIDDK
                     EVGLAAEGKPFNEALLGAAEEYAIECAILKVNGSETLDFIVDEGVQIHGGNGFSDEYI
                     ISKAYRDSRINRIYEGTNEINRLLTVDMVLKRAMKGRLDIMGAAMVVSKELMSIPDFG
                     GGEDTPFAKERRIIQQFKKAILMTAGAAVQKLMMKLEQEQEILMNIADMAIQTFVAES
                     ALLRAMKLADQRGEDGAAFELDIMRTFLYDAADSINKSGKDAINAFADGDEQRMMLLG
                     LKRFTKVDAFNSKAARRRIAEKLITDNRYPL"
     gene            3610..>4484
                     /locus_tag="VGQ51_09620"
     CDS             3610..>4484
                     /locus_tag="VGQ51_09620"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV9036870.1"
                     /translation="MRTVTLRPHTGLIILLLFLQAFAAHGQDSVWLARTTGQLPFMEY
                     GIGDDRLGGAKIGFLDSNILVKVVDSFRTDYKVRLSTQHYAYIAKTSLILLNKELNLP
                     VPHNPHLTGSMQVFGDTASDYVQLALDDRYPYRSWQLIDPSRVAVDVFGVTSNTNWIT
                     QLHTAKEIKNTWYEQVEDDVFRIYIQLKHSQHWGYAISYDSIGRHLVIRIRRQPPLDI
                     KRLRIAIDAGHGGENVGASGGTSRVLEKEYTLLIAEQLRKVLKTAGVKAVFMTRTKDT
                     TLSMPERIEMLKQFDP"
BASE COUNT         1154 a         1194 c         1246 g          890 t
ORIGIN      
        1 cccccacccg aaaacgaccg tatggtctcc gccgtaccat ctgtggaatg atcgtccagc
       61 acgatgactt cgaaccggtc gcgcggatac gactgccttg cgagggatcc gagacagccg
      121 gcaatgttcg cttcttcgtt gcgtgcaggt atcagcacgg tgatggtcgc ttgcgccgtc
      181 gccgcctgtc gaacgaatac cggaatggcc ttccatgccc ggtgatacgc gaggatcaat
      241 cccgcataaa cgaggaaaat taaaccgacc ggcaagacga gcagccaaat catacgaatc
      301 aaatgatcgg taaatatagc gcggaaacaa tgatttccat gaatttagac tggagcagct
      361 ggtgccccgc gggcagcacc gtgagctggc aatacggggc gcaacgtctc tgaaacgtct
      421 gtccgcgttg tgaggggatg atccggtcga accggccata caacaaatgg acgggtatcc
      481 ggcgacggcg aacgatgtcg ctgacaggcc ccggacgagg gttgaatccg ctcattaccg
      541 tccagcgggt atacaggagg cgacgcgctt ccgcatcgtc gatataatgc agggtaaatt
      601 tgtaaacgct ggggttgacc atgcgcagat aattagccgc gcgaagcaaa aagaagaacc
      661 agccgggatg tcgcatagta tagctgaaaa gaccattccc gggtctcgtc tgcgtggaca
      721 accaatacca cgggttgacg cgcaaaccat ccggcgccag taaaaccagc caccggatct
      781 tttcgggcgc cagctcggcc agttgcaagg cgacccttcc gcccatgctg taaccaatca
      841 gcgtccattc ttcatttccg ccggcaagct ccgacgcaat gtcaacgagc agggacagca
      901 gatccggggg tgcaaataaa atctcttcct tccattccgt ctccccatga aaaggcatat
      961 cgatggcgat catcgtaaag tcctgagcca gcgctgctga caggaagccg aatgaggccg
     1021 ccgattcccc atagccgtga aaagcgaaca ggagccggcg ccccgtgccc cagcgggaat
     1081 aatgaaaacg cgaagaccgg aaagaaacga aatgttccga catggggccc tttatagccc
     1141 gaatgtacgg acttccacgg gcctcggaac ggcctttcgg acaagaaaaa aataccccgg
     1201 atcttttcgg gtagttccaa aaaatccgta cttttaatta gttgtttaac taaccgtatg
     1261 ccaaacaacc aattcagaag gggcgagctt tatagtttta tcaccggcaa ggcgagcacg
     1321 gccattgcgc gcaaccttca aaaaaggttc aatgcggcag ggttgaagat cacgatagaa
     1381 cagtggagtg tactctatca tctctggaaa gaagatggta agagtcagca ggagctttgc
     1441 agcgccacct ttcgggataa acccagtatt acgaggctgg tggacaacct ggagcggcaa
     1501 cagctggtca aacgggtccc gtcgcaggac gacagacgga tcaacctgat ctatctgacg
     1561 cgccaggccc tgcggctgca ggaagaaacg atgcagttgg ccgaagcgac attgaatgaa
     1621 gcgctggaag gagtgcccgc cgaccggatc gagatctgta aagaggtgct acaagtcgtt
     1681 tatgacaatc ttcgatagat catttcattt caaaactatt atctatgagc acgtcaactg
     1741 gaaaaacaca agccctcaaa ggagccgaat ggctgatcag ggaaagctcc cccttcgata
     1801 cgttcattcc cgaagactac aatgaagagc agcggatggt caaagatatg tgcaatgcct
     1861 ttctggatgc cgaggtgctg cccatcatcg accggatcga caagctcgag ccgggactca
     1921 tgccttcgct gatcgccagg gcgggggagc agggactgct gggtgcgtcc attcccgagg
     1981 aatacggcgg tttgggaaag gattttatta cgtcgacgct ggtgaatgag ggtctgggag
     2041 gaggtttctc gttctccgtt ggcgtgtccg cacatacggg catcggtaca ctgcccatcc
     2101 tgtatttcgg cacaccggaa caaaaacaga aatatatacc caggctagca agcggagaat
     2161 ggaagggttc ctacggcctg acagagccta acagcggcag tgatgctttg ggcgccaaga
     2221 ccacggcgaa gcttacgtcc gatggaaaat attacctgct caacggtcag aaatgctgga
     2281 tcacgaacgg cggtttcgcg gatgtctata ccgttttcgc gaagatcgac ggggacaagt
     2341 tctccgcatt catcgtcgaa cgcggattcg aaggttttac acagggaccg gaagagcata
     2401 agatgggaat aaagggttct tcgacggtgc aattgtattt tcaggattgt aaagtgccgg
     2461 tagagaactt gctgggagag atcggtaagg gtcatatcat cgctttcaat atcctgaata
     2521 tcggtcgtct gaaattgtgc gctgccgcat tgggaggcgc taaaagagca actaccattt
     2581 cgatcgaata tgcgaatacg cgcgaacagt tcaaaacgcc catctcccgg ttcggtgcta
     2641 tccagcacaa gctggccgca atggccatcc gcatgtggac gacggaaagc gctctttatc
     2701 gcacggcgaa gttgatcgac gacaaggagg tcggactcgc cgcggaaggg aaacccttta
     2761 acgaggcgct gctcggcgcc gccgaggaat atgccatcga atgcgccatc ctcaaagtaa
     2821 atggcagcga aacacttgat ttcatcgtgg acgaaggcgt tcagatccac ggcggcaacg
     2881 gattcagcga cgaatacatc atctctaagg cctatcgcga cagccggatc aaccgcatct
     2941 acgaaggaac caacgagatc aaccgtctcc tgacggtgga catggtgctc aaacgtgcta
     3001 tgaagggtcg gctggacatt atgggcgcgg ccatggtggt ttcgaaggag ctcatgagca
     3061 tccccgattt cggaggtggc gaagacacgc cttttgcaaa ggaaagaagg atcatccagc
     3121 agtttaaaaa agcgatcctg atgacggcgg gagccgcggt gcagaagctc atgatgaaac
     3181 tcgagcagga gcaggagata ctgatgaata tcgccgatat ggccatccag accttcgtgg
     3241 cggagagtgc tttgctgagg gccatgaaac tcgcggatca gcggggcgag gacggcgccg
     3301 catttgaatt ggatattatg cgtacctttc tatacgatgc ggcggacagc atcaataagt
     3361 ccggcaagga cgccatcaac gccttcgccg acggggacga gcaacgaatg atgctgctgg
     3421 gtctgaaacg gttcacaaag gtggatgctt tcaacagcaa ggccgcgaga cgcaggatcg
     3481 ccgaaaaact gatcactgac aatagatatc cactgtagta ctgcgagtca tacatgagag
     3541 cacgacggcg ttcggttttc cgggcgccgt tttcttttgg tggccggctg agttgccgta
     3601 tctttcagca tgcgaacagt taccctacgg ccgcataccg gccttattat cctactcctc
     3661 tttctgcagg ccttcgcggc tcatggccag gacagcgtct ggctggcacg caccaccgga
     3721 cagctcccct tcatggaata tggcatcggc gacgaccggc tgggcggcgc caaaattggc
     3781 tttctcgact ccaatatcct tgtgaaggta gtcgacagct ttcgcacgga ctacaaggtc
     3841 agactttcca cccagcacta cgcctatata gcgaaaacca gtcttatctt attgaacaaa
     3901 gaacttaatc ttcccgtccc gcataacccg catctgaccg gcagcatgca ggtctttggc
     3961 gatacggctt cggattatgt acagctggcg ctcgacgatc gctatcctta ccgcagctgg
     4021 caactgatcg atccatcccg ggtggccgtg gacgttttcg gagtgactag caataccaac
     4081 tggatcacac agctgcacac cgccaaagag atcaaaaata cgtggtacga gcaggtcgag
     4141 gacgatgtat tccggatcta tatccaactg aaacactccc agcactgggg ctatgccatt
     4201 tcctacgaca gcatcgggcg ccacctggtg atccggatcc gccgtcagcc ccccctcgat
     4261 atcaaacggc tcaggatcgc catcgatgcc ggacacggag gcgagaacgt gggcgccagc
     4321 ggcgggacaa gcagggtgct ggaaaaagaa tataccctgc tcatcgccga acagctccgg
     4381 aaagtactca agaccgcggg tgtgaaggcc gttttcatga cacgcacaaa ggataccacg
     4441 ctgagcatgc ccgaacggat cgaaatgctg aaacagttcg atcc
//
LOCUS       DASYSV010000323         4212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_446318, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000323 DASYSV010000000
VERSION     DASYSV010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446318"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VGQ51_09625"
     CDS             complement(<1..518)
                     /locus_tag="VGQ51_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV9036871.1"
                     /translation="MSRDLKLLVRETARRREVPRKTRLLSPGETCQHLFYIEKGMLAC
                     FDQEGGKSYCTWIMTEKDIATSVDSFNNQVFSTEWIETVEDCVLWTLSKQELEMISDM
                     HLEFRIIRQQLTDKYHIQCRTLDAQRKRPPDHFYDYLVQQFPEIVRRTPNHILASYMG
                     VVESTLYKIKKE"
     gene            722..2023
                     /locus_tag="VGQ51_09630"
     CDS             722..2023
                     /locus_tag="VGQ51_09630"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV9036872.1"
                     /translation="MTPSSRKRIVIVGAGFAGLRLARSLNNTPYDVYLIDRHNYHQFQ
                     PLMYQVASARLEPSSISFPLRKVFQHSNNVTIRIAEVDHVDTTAKVLYTGVGDISYDY
                     LVLALGCTTNYFNNASIRKYSFPMKSVPNAMALRNRILQTFEDTIEAGPAELHALLNF
                     VIVGGGPTGVELAGAMAEMKKYILPRDYPEVDFSRFTIYLLEGSPNTLNSMSDASKRK
                     SREYLEKLGVIVRTGTVVEDYDGVTLKLKGGEILPARNVIWAAGVIANKLNGLPETSL
                     VRGNRLKTNRYGEVEGLEGVYAVGDLAYMPTPKYPNGHPQLANVAIAQAGHLAGNFKR
                     EAKGRSREEFEYHNKGTMATVGKRKAVVDLPGFSFQGRLAWFTWMFVHLMLLLNVRNK
                     LIVFFNWTMSYFANDSTLRLMLKADRIDSPFEAVEAPPVKG"
     gene            2078..2767
                     /locus_tag="VGQ51_09635"
     CDS             2078..2767
                     /locus_tag="VGQ51_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9036873.1"
                     /translation="MAEIIVELKHANIFQGDNLILQDVNLSVDKGQFVYLVGKTGTGK
                     SSLLKTLYGDLPLREGEGQVAGFDLREMDWKKVPYLRRTLGVVFQDFQLLTDRDVNDN
                     LKFVLRATGWKDIRLMDEKVSDVLDKVGLKAKGFKMPFEMSGGEQQRVDIARALLNSP
                     KLILADEPTGNLDPETSDEIMQLLFRIARDYGTTIMMATHDYRVINKFPARMVRTERG
                     AVAEIASITVD"
     gene            complement(2799..3962)
                     /locus_tag="VGQ51_09640"
     CDS             complement(2799..3962)
                     /locus_tag="VGQ51_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase"
                     /protein_id="HEV9036874.1"
                     /translation="MNDHLHIVCLDVPYPVDYGGVFDLFHKIRLLSQAGIKIHLHCFE
                     YGRGPQPVLDEYCVEVHYYARHEGHKGFSHRLPYIVCSRSNPQLLDRLLKDDYPILLE
                     GIHCTYLLNDQRFDGRRILLRLHNVEYRYYRELYRSERSLLKKLYYLHESILLRAYER
                     RIAGKVKILAVSEKDSEVYKREFGATDIEVLPVFLPFDQVQSKEGTGCFCLYHGNLSV
                     AENEKVVTWLLKNVFGSLEIPFIISGKNPSPRLTRRIYQYPHTCLIPNPTEGELQDLI
                     SKAQVNILPSFNCTGIKLKLLNALFNGRHCIVNPGAVEGSGLQTTCHMADCAVGIRDL
                     IGQLSGRPFTSEEIAQRGKILGVLFDQEHNLRKLMQCIWPAGMAAAGAAAATG"
     gene            complement(3964..>4212)
                     /locus_tag="VGQ51_09645"
     CDS             complement(3964..>4212)
                     /locus_tag="VGQ51_09645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9036875.1"
                     /translation="MHAGIPQIAVGYPAYREINNSCPIAVLIDEPGVREISEALNQLL
                     NNTQLYQTLLTNCRQARLRYNWQEEEQKLVLLYQRLLN"
BASE COUNT          965 a         1068 c         1198 g          981 t
ORIGIN      
        1 ctttcttttt tgatcttata aagtgtcgac tctaccacgc ccatatagga agcgaggata
       61 tggttgggcg ttcgccggac gatctccggg aattgctgca cgaggtagtc gtaaaaatga
      121 tccggcggcc gtttgcgctg agcgtcgagc gtacggcact ggatatgata tttgtcggtt
      181 agctgttgcc gtatgatgcg gaattccagg tgcatgtcgg atatcatttc gagctcttgt
      241 ttggagaggg tccacaggac gcagtcttcc accgtctcga tccattcggt cgagaagacc
      301 tggttattga agctgtccac ggaagtggca atgtcctttt ccgtcatgat ccaggtacaa
      361 tagctcttgc cgccctcctg gtcgaaacag gcgagcatgc ccttttcgat atagaaaaga
      421 tgctgacagg tctccccggg tgataacaac cgggtcttcc tgggcacctc tctccggcgg
      481 gcggtctcgc gaacgagtaa tttcaggtcc ctggacatgt ggtgaacaga ttccaggtgg
      541 tcaatgattt cattcatcat aacggtctgg ggtagagatc accaagatat tcccgttccc
      601 tgtatccctc gcgcagtgtt tgggtggatt tcgcattacc cgtacctttg tggtggaatt
      661 tggttgcgga gaattcgatg caaggacagt ggtagtggaa cggcagtcta aataatagct
      721 tatgacaccc agtagcagaa agcgtatcgt catagtgggc gccggttttg caggtttgcg
      781 tttggccagg agcctcaaca atacgccgta cgatgtttat ctgatcgaca ggcataatta
      841 tcatcaattc caacccctca tgtaccaggt ggcgagcgcc aggctggaac catcgagcat
      901 ctcctttccc ctgcgaaagg tattccagca cagcaacaat gtaacgatac ggatagccga
      961 agtggatcat gtcgacacga ccgcaaaggt cctgtacacc ggcgtcgggg acatttcgta
     1021 cgactacctt gtcctggcgc tgggatgtac caccaattat ttcaataacg ccagtataag
     1081 gaaatattct ttcccgatga aatcggtgcc caacgccatg gcgcttcgga acaggatcct
     1141 ccagacgttt gaagatacca tcgaagcggg gcccgcggag cttcacgcat tgctgaactt
     1201 cgtcatagtc ggcggggggc ctaccggggt ggagctcgcg ggcgccatgg ccgagatgaa
     1261 gaaatatatc cttcccaggg attatcccga agtggatttt tcccggttca ccatctacct
     1321 gctggaaggt tcaccgaaca ccctcaactc catgagcgat gcctccaaaa ggaagtcccg
     1381 ggaatacctg gagaagctgg gcgtcatcgt ccggacgggc accgtcgtgg aagattatga
     1441 cggggttacg ctgaagctga aggggggcga gatcctgccg gcgaggaacg tgatatgggc
     1501 ggcgggcgtc atcgccaaca agttgaatgg cctgccggaa acaagcctgg tacggggcaa
     1561 caggctcaag accaaccgct atggagaggt ggaggggctc gaaggagttt atgccgtcgg
     1621 ggatcttgcc tacatgccca ctccaaaata tcctaacggg catccgcaac tggcaaatgt
     1681 agccatcgca caggccgggc atctggccgg gaatttcaaa cgggaagcga agggccggag
     1741 ccgggaggaa ttcgaatacc ataacaaggg aacgatggcc accgtaggta agcgcaaggc
     1801 cgtggtcgat ctgccgggtt tttctttcca gggccgtctg gcctggttca cctggatgtt
     1861 cgttcacctg atgctgctgc tcaacgtgcg caacaagctg atcgtttttt tcaactggac
     1921 gatgagctat tttgccaacg atagtacatt acggctcatg ctgaaggccg accgtatcga
     1981 ctctccgttc gaagccgtag aggcgccccc ggtaaaaggg tgatgagcag accttttttt
     2041 ccttattttt gtttggataa aaacacaagt ggaacctatg gccgagatca tcgttgaatt
     2101 aaaacatgct aatatctttc agggcgataa tctgattctc caggacgtca atctctccgt
     2161 cgacaaaggc cagttcgttt atttggtagg caagaccggc acaggcaaat ccagcctgct
     2221 caagacgctg tacggcgatc tcccgttgcg cgaaggggag ggccaggtag cgggtttcga
     2281 tcttcgggaa atggattgga aaaaggttcc ctatctccgc cgtacgctgg gcgtggtctt
     2341 ccaggatttt cagctgctga cggatcgtga tgtcaacgat aacctgaaat tcgtattacg
     2401 ggccacgggc tggaaggaca tccgtttgat ggatgaaaaa gtctccgacg tcctggataa
     2461 ggtaggactg aaggcaaagg gttttaaaat gcctttcgaa atgagcggtg gcgagcagca
     2521 acgggtggac atcgcccggg cgctgctgaa ctctcccaag ctgatcctgg ccgacgagcc
     2581 tacgggcaat ctcgatcccg agacttcgga tgagatcatg cagctgttgt tccgcatcgc
     2641 ccgcgattac ggcacgacga tcatgatggc tacacacgac taccgggtca tcaataaatt
     2701 tcccgccagg atggtgcgga ccgaaagggg agcggtcgca gaaattgcca gcatcactgt
     2761 ggattagact gagtggacag ggaaggcggg gactactttt agccggtggc ggcagctgcg
     2821 ccggcggcag ccataccggc aggccagata cactgcatca gtttccgaag attatgctcc
     2881 tggtcgaaca gcactccgag gatctttccc cgctgggcga tctcctccga agtaaagggc
     2941 cgtcccgaca gttgtccgat gagatcccgg atccctaccg cacaatccgc catatgacag
     3001 gtggtctgca gacccgagcc ttctaccgcg cccgggttga cgatacagtg tctgccgttg
     3061 aagagggcat tcagcagctt gagctttatt cccgtgcaat tgaacgatgg caggatattg
     3121 acctgggcct ttgagataag gtcctgcagc tcgccctcgg tgggattggg gatgagacag
     3181 gtgtgcgggt actgatagat gcggcgtgtc aggcgtggcg aagggttctt gccggagatg
     3241 atgaagggga tctccaaaga gccgaataca ttcttcaaca gccatgtgac aactttttcg
     3301 ttttccgcca cggacagatt gccgtgataa agacagaaac aacccgtccc ttccttgctc
     3361 tgcacctgat cgaagggtaa gaagactggt aagacctcga tatcggtcgc cccgaattcc
     3421 cgtttgtata cttcactgtc cttttctgaa acggcgagga tcttcacctt gcccgcgatg
     3481 cgccgctcgt atgcgcgaag cagtatgctc tcgtggagat agtatagttt cttcagcagc
     3541 gaccgctctg accggtagag ctcgcggtag taccggtatt ccacgttgtg aagacgaagg
     3601 aggatgcggc ggccgtcgaa tcgctggtcg tttagcaggt aggtacaatg aatgccttcc
     3661 agaaggatcg ggtagtcgtc tttcaacagc cggtcgagca gctgcgggtt cgatctcgaa
     3721 caaacgatat agggtagccg atgtgagaag cctttatgcc cttcatggcg ggcatagtaa
     3781 tgcacttcca cacagtattc gtcgagcacc ggctggggcc cccggccgta ttcgaaacag
     3841 tgcagatgta tctttatgcc cgcctgtgag agcaacctta tcttatgaaa gaggtcgaat
     3901 acgcccccgt aatccacagg atagggcaca tccaggcaca cgatatgtaa atggtcgttc
     3961 acgtcaattc aggagtcgtt gatagagcaa caccagtttt tgttcttctt cctgccagtt
     4021 gtagcgaagc cgggcctgtc tgcagttggt taataacgtt tgatatagct gcgtattatt
     4081 cagcaactgg ttcagcgctt ccgatatttc ccggacgccg ggctcgtcaa ttagaacagc
     4141 gatggggcag gaattgttta tttcccggta agcaggatat ccgacggcta tctgagggat
     4201 ccctgcgtgc at
//
LOCUS       DASYSV010000324         7955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1860624, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000324 DASYSV010000000
VERSION     DASYSV010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860624"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VGQ51_09650"
     CDS             complement(<1..1280)
                     /locus_tag="VGQ51_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV9036876.1"
                     /translation="MYFESKNRSNEELIRLYKSILLPRMIEEKMLVLLRQGKISKWFS
                     GIGQEAIAIGATLAMAPEEWILPLHRNLGVFTGRGMPLSKLFLQWQGSEDGYSKGRER
                     SFHFGSREHAICGMISHLGPQLGVADGVALAYKLRNQKAATLVFSGDGGTSEGDFHEA
                     LNVASVWDLPVIFLIENNGYGLSTPVGEQYRCESLVYKAEGYGMEGVRIDGNNILTVY
                     DTIQGIRDYCIKYQRPYLVECMTFRMRGHEEASGIKYVPPQLLEDWAKKDPVMKYESW
                     LREQGILDDTTAQSIRDEIRGYIDAELSEASGPIHIFADSEQELDDVYAPPSVTAVRP
                     IEEDGGNGGPQRKMIEAIREGLDQSMREHDNLVLMGQDIAEYGGAFKVTDGLMDKYGK
                     GRVRNTPLCESAIVGCTLGLSLEGFKSVMEMQFA"
     gene            complement(1324..1686)
                     /locus_tag="VGQ51_09655"
     CDS             complement(1324..1686)
                     /locus_tag="VGQ51_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV9036877.1"
                     /translation="MNFLLRVIITAVVAFALSSVLPGIHIDTFWTAIVLSLVLALLNI
                     LLKPILIILTLPITIVTLGFFLFIINALIVLLASDFVRGFVVDSFWWALLFSLLLSLL
                     TSLLTKKDRNREEDTRRY"
     gene            complement(1719..3026)
                     /locus_tag="VGQ51_09660"
     CDS             complement(1719..3026)
                     /locus_tag="VGQ51_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV9036878.1"
                     /translation="MEVKTSAKLNRAIAPPIKDAVEFELNLPPYRKTLLSNGVEVYAI
                     DLGNVEAMMVSWIFDAGNSWEEKKGIAAAANGLLKNGTTDRTAFAINQHFEYYGAYLN
                     RAAQHETAELTLHCLNKYVGELLPVVAELISDSIYPGEELDIYKRNSQQRLQVSLKKS
                     DFVANRLIDAYLYDEKHPYGKYALMEDYQALEREDLVAYFDRYYRHGACRILAAGRLP
                     DDLIQRLERSFGNLPLRPPVAYGSLPAPAIIPAATKKYNIVNDPNGVQGSIRIARNFP
                     NRHHPDFQKVQVLNNVFGGYFGSRLMANIREDKGYTYGIHSWLVNNIQASALMISTEA
                     GKEVCAPAIEEVYKEMELLRSELIEEEELQMARNFTIGTILGDLDGPFHVAGRWKNIL
                     LNGLDGNYFNEGVRIIKTITPEELQELAVKYLDPAVFYELVVV"
     gene            complement(3032..4273)
                     /locus_tag="VGQ51_09665"
     CDS             complement(3032..4273)
                     /locus_tag="VGQ51_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV9036879.1"
                     /translation="MIKYDRFQLDNGLRVLVHEDASTPMVVVNVLYDVGARDEDPKKT
                     GFAHLFEHLMFGGSVNIDDFETPLQLAGGENNAYTTNDFTNYYIQLPAENIETAFWLE
                     SDRMLSLAFSEKSLEVQRKVVMEEFKEHYLNKPYGDVWFKLRELVYKEHPYRWMTIGS
                     ELSHIEQARLEDVKGFFFKYYRPVNAILVVAGKVNTAQARSLAEKWFGDIPAGDKYSR
                     RLPHEPLQREARKLTIHAQVPLDALYKCYPMASRTEPGYYVADLLTEVLGGGASSRLH
                     QALIKEKRLFSQIDCYHTGSVDPGLVVIEGRLIKGVSMEDADKAVEEELHRLQNELVD
                     EKELEKVKNKTEAAIVFEDMSVMNRANSLAIYELLGDVNMMNTELEKYRAVTAGAMLE
                     TARRIFDPLNSNTLYYLSETK"
     gene            complement(4347..5498)
                     /locus_tag="VGQ51_09670"
     CDS             complement(4347..5498)
                     /locus_tag="VGQ51_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEV9036880.1"
                     /translation="MKQSLVAQETFAQPPSKLITSFPITIFTGGVILLRATLDNFPDS
                     LNFILDTGSGGISLDSVTCEQLKLKPLPSDKTILGIAGIRQVRFLYDRTLHLPNLTVD
                     SLNFHVSDYDILTQVYGARIDGIIGYSFFSRYIVDINYDSNRVYVYSRGSFRYPHGGY
                     MLRPVIASLPIIGADLMDIQKVTGRFYFDTGAGLCTLLSTDFVTDSNFLDGHKKSYYT
                     QAQGLGGKAIMRMTTLKEVKVGRYRFRRVPIYIFDDQYNVTSYPNLGGLIGNDILRRF
                     NVILDYGRRMIYMTPNSHYRDPFDYSYTGLGMYWVDGEIRVGDVMKDSPAEKAGLKED
                     DVIIAVGNNFSNNIQVYKSLLQNAGEKVKIIVKRGTSLEQMVLRVKSIR"
     gene            5691..6815
                     /gene="mqnC"
                     /locus_tag="VGQ51_09675"
     CDS             5691..6815
                     /gene="mqnC"
                     /locus_tag="VGQ51_09675"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEV9036881.1"
                     /translation="MSLSTFYEKALRREFLDIEEGVTLFREAPLTELMAVADELRKSA
                     VPHGKVTWQIDRNVNTTNVCIANCKFCNFYRIPGHADAYITDIDTYKRKIEETFRWGG
                     DQLLLQGGHHPELGLSFYTNLFRQLKELYPALKLHTLGPPEVAHICKLEKMSHHAVLS
                     ALRKAGMDSLPGAGAEILVDRVRRLISKGKCGAREWLDIMHEAHNLHITTSATMMFGH
                     VETLHERMEHLVKIREVQSRKPENAKGFLAFIPWTFQDVDTLLTRIRGVHNLTTPEEY
                     IRMIAISRIMLPNVKNIQASWLTVGKQTAQLCLHAGANDFGSIMLEENVVSAAGAPHR
                     FTAKGIQEAIRGAGFEPQLRNQQYEWRSLPDVMEEQVINY"
     gene            7010..>7955
                     /locus_tag="VGQ51_09680"
     CDS             7010..>7955
                     /locus_tag="VGQ51_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV9036882.1"
                     /translation="MKSPFRKLVRISVVLLVLVLLFNFFGYYINRIRSLENKELIEAI
                     NRSGHLQTLSQSIAKESILLLNTSDQRTASMVRDTLGRLIPEFQGSQALLQQQAGSTP
                     SPVPQQIFQIKLLLSTSQPFFEAVTAIGQELTQSDSLLLAMNKKLYLRNLLYNEAKYN
                     SLMGEINQHYSTLVNETNGEVATIDTGKLISLIAAILGLIILVLEPAFKKGEKNYNEL
                     QKAKNDLLHESKFLASILRSQTNYVIRINRAGQFTYANPAFLKTFGYTEEEIQHILFY
                     TTIFPKDMARCQQVASECWNNPGKVSRLLIRKPIGRSRE"
BASE COUNT         1858 a         2236 c         1991 g         1870 t
ORIGIN      
        1 tcggcgaact gcatttccat gacgcttttg aaaccctcca ggctcagccc caatgtgcag
       61 cccacaatgg cgctttcgca caacggcgta ttccggaccc tgcccttacc atacttatcc
      121 atcaacccgt cggtgacctt gaaggcgccc ccatattcgg cgatgtcctg tcccatcagg
      181 acaaggttat cgtgttcgcg catcgactgg tccagacctt cgcggatggc ctcgatcatc
      241 ttccgttgag ggccgccatt ccctccgtcc tcctctatcg gacgtacggc ggtgacagag
      301 ggcggcgcat agacgtcgtc caattcctgt tcgctatcgg caaaaatgtg gataggaccc
      361 gatgcttcgc tcagttcggc atcgatgtat ccgcggatct catcacgtat cgactgcgcc
      421 gtggtatcgt ccagtatgcc ctgttcacgc agccagctct catatttcat taccggatcc
      481 tttttggccc agtcttcgag cagctgtggc gggacatatt tgatgccgct ggcctcctcg
      541 tgtccgcgca tccggaaggt catgcactcc acgagatagg gacgttggta tttgatgcag
      601 taatcccgaa tgccctggat cgtatcgtag acggtgagga tattgttccc gtcgatacgc
      661 actccttcca tcccatagcc ctccgcctta tagaccaggc tttcgcagcg atattgttcg
      721 ccgacaggcg tactcagccc gtatccattg ttctcgatga gaaatatgac cggcagatcc
      781 catacggacg ccacgttcag agcttcgtgg aaatcgcctt cgctggttcc tccgtcgccg
      841 gagaatacga gggtcgccgc cttttgatta cgtaatttat aggccagggc tactccgtct
      901 gccacaccaa gctgcggtcc aagatgcgag atcataccgc agatcgcatg ctcgcggctg
      961 ccgaaatgaa agctccgctc ccttccctta ctgtaaccgt cctcactgcc ctgccactgc
     1021 aggaagagct ttgataacgg catcccccgg ccggtgaata cgccgaggtt acggtgcagc
     1081 ggcaggatcc attcttccgg cgccatggcc agggtcgcac ctatcgcgat cgcttcctgc
     1141 ccgatgccgc tgaaccactt gctgatcttg ccctgtctca ataggaccag cattttctct
     1201 tcgatcatcc ggggcagcaa aatacttttg tagagacgga tcagttcttc gttgctacgg
     1261 tttttacttt caaaatacat acgggctggt tatggcgcgg acctatgctc cgcgtttctt
     1321 atttcaatat ctcctggtat cctcttccct gttcctgtcc tttttggtca acaacgaggt
     1381 cagcagcgac agcagcaggc tgaacagcag tgcccaccag aaactgtcga ccacgaaccc
     1441 acgcacgaaa tcgctggcca gcagcacgat cagcgcattg atgataaaaa ggaagaaacc
     1501 taaagtaacg atagtgatcg gtaaagtaag gatgataagg atgggtttca acagaatgtt
     1561 cagcaacgca agcaccaatg acaacacgat cgccgtccag aaagtatcga tatgaatgcc
     1621 cggcaaaacg gaagacagcg caaaagctac caccgccgta atgatgacgc ggagcagaaa
     1681 attcatgtga ttcttttgct caagttacgc cattctcatc aaaccactac gagctcatag
     1741 aacaccgccg gatcgagata tttcaccgcc agctcctgca gctcttccgg tgtgatcgtc
     1801 ttgatgattc gcaccccctc attgaaataa ttgccatcca acccgttcaa caggatattt
     1861 ttccaccgcc cggccacatg aaaaggcccg tcgagatcac ccagaattgt cccgatggta
     1921 aaattccggg ccatctgcag ctcttcctct tcgatgagct ctgagcgcag cagttccatt
     1981 tccttataga cctcttcgat cgccggggcg cagacctctt ttcccgcctc ggtgctgatc
     2041 atcagcgcgc ttgcctgaat attgttgaca agccaactat ggatgccata ggtgtacccc
     2101 ttgtcttcgc ggatattcgc catcaggcgg gacccgaaat agccgccgaa cacattgttc
     2161 agcacctgca ctttctggaa atccggatga tgacgattgg ggaaattgcg ggcgatccgg
     2221 atcgatccct gaacgccatt gggatcgttg acgatattgt atttcttcgt cgccgccgga
     2281 atgatcgccg gggcaggaag cgacccatag gctacgggcg gcctcaaggg cagattgccg
     2341 aaactcctct cgagccgttg gatcagatca tcgggaagcc ggcctgcagc caggatgcgg
     2401 caggcgccat gacgataata acggtcaaaa taagccacca ggtcttcgcg ctccaacgcc
     2461 tgataatctt ccatcagcgc gtattttcca tagggatgct tttcatcata gaggtaagca
     2521 tcgatcagcc gatttgccac gaaatcgctt ttcttgagac tgacctgcag acgctgctgg
     2581 ctatttcttt tatagatgtc cagctcttcc ccgggataga tggaatccga gatcagctcg
     2641 gccaccaccg gcaacagctc gccgacatat ttattgagac aatgcagcgt gagctcggcg
     2701 gtctcatgtt gcgccgccct gttcagatag gcgccataat attcgaaatg ctggttgatc
     2761 gcaaaagcgg tccggtccgt agtcccattt ttcagcaggc cattggccgc ggcggcaatg
     2821 cccttttttt cctcccacga attgccggca tcgaagatcc agctgaccat catcgcttcg
     2881 acattgccca ggtcgatggc atatacttcc acgccattgg aaaggagggt tttccggtaa
     2941 ggcggcaggt tcagctcgaa ttccaccgca tccttaatgg gcggcgcgat ggccctgttc
     3001 aatttagcgc tcgtctttac ttccatgcca ttcattttgt ttcgctgagg taataaagtg
     3061 tgttactatt gagcggatcg aagatccggc gggcggtctc cagcatggcc ccagcggtga
     3121 cggcccggta tttttccagc tcggtattca tcatattcac atcccccaat aattcgtata
     3181 tcgccaggct attggcgcga ttcatgacgc tcatgtcttc gaacacgatc gcagcctcgg
     3241 ttttgttctt taccttctcc agctcttttt cgtctacaag ttcattctga agccggtgca
     3301 gctcttcttc cacggcctta tccgcatctt ccatactcac ccctttgatc agccgccctt
     3361 cgatgaccac cagcccggga tccacagacc cggtatgata gcaatcgatc tggctgaaca
     3421 gacgtttctc cttgatcagt gcctgatgca gccgcgaaga ggctccgccc cccagcactt
     3481 cggtgagcag atccgcaaca taatatccag gttccgtacg ggaagccatc gggtagcatt
     3541 tatacagggc atccaggggc acctgggcat gtatcgtcag cttgcgcgct tcgcgctgca
     3601 gcggctcatg cggcagtcta cggctatatt tatctccggc ggggatatcc ccgaaccatt
     3661 tttcggccag actgcgagcc tgggccgtat tcacctttcc ggcaacgacc aaaatggcgt
     3721 tgacaggccg gtaatatttg aaaaagaatc ctttgacatc ttcgagccgc gcctgctcga
     3781 tatgactgag ctcgctgccg atggtcatcc agcggtacgg gtgctcttta tataccagct
     3841 cccgcagctt gaaccagacg tcgccgtaag gtttgttcag gtaatgctcc ttgaattctt
     3901 ccatcacgac cttccgctga acttcgaggc ttttttcact gaaagcaagc gataacatcc
     3961 ggtcgctctc cagccaaaaa gcagtctcga tattctccgc gggcaattgg atatagtaat
     4021 tcgtgaagtc gttggtcgta taggcattgt tctcgccgcc cgcgagctgc agcggtgttt
     4081 cgaaatcgtc gatattgacg gaacctccga acatcaggtg ttcgaagaga tgggcaaagc
     4141 ctgtcttttt cggatcttca tctcgggcgc cgacatcata caggacgttg acgacgacca
     4201 tcggggtgga agcgtcttca tgtacgagca cgcgcaatcc gttgtccagc tgaaacctgt
     4261 catacttgat catagtaaag tccttgaccc catcaacgcc cgcctaaccg gccgggcctt
     4321 gggggaaggt gcaatttaat gaaaatctag cggatgcttt tgacccggag gaccatctgt
     4381 tcgagcgatg tccctctttt gacaatgatc ttcacctttt ccccggcgtt ctggaggagg
     4441 ctcttataga cctgtatgtt attgctgaaa ttgttgccga cggcgatgat gacgtcatcc
     4501 tccttcagac ccgccttttc ggcaggcgag tccttcatta catcccccac gcggatctcg
     4561 ccgtccaccc agtacatgcc gagaccggta taagaataat cgaagggatc cctgtaatgc
     4621 gagttaggag tcatatagat catccggcgg ccataatcca ggatgacgtt gaaccgccgc
     4681 aaaatatcgt tcccgatcag tccccctaaa ttgggatagc tcgttacatt atactgatcg
     4741 tcaaagatgt atatcggtac ccgtcgaaaa cggtagcgcc ccacctttac ttctttcagg
     4801 gtggtcatac gcatgatggc ctttcctccc aacccctgag cctgcgtata ataggacttt
     4861 ttgtggccgt ccagaaaatt gctgtccgtg acgaaatcag tagaaagcag ggtgcagaga
     4921 ccggccccgg tgtcgaaata aaaccggccg gtcacctttt ggatatccat caggtcggcg
     4981 ccgatgatcg gcagcgaggc gatcaccggt cgcagcatat atcccccgtg cgggtagcgg
     5041 aaggacccgc gggagtaaac gtagacccgg ttggaatcgt aattgatgtc tacgatatag
     5101 cgggaaaaaa agctataccc gatgatgccg tcgatcctgg caccgtatac ctgggtcagg
     5161 atgtcgtagt cgctgacatg aaagttcaga ctatctaccg taagattggg aagatggagg
     5221 gtccggtcat acaggaacct cacctgccgg ataccggcaa tgcccaaaat cgtcttatcc
     5281 gaaggcaaag gtttcagctt tagctgctcg caggtgacgg aatccagcga gatgccgccg
     5341 ctgccggtat cgaggatgaa attgaggcta tcgggaaaat tgtccagcgt ggcccgtaac
     5401 agaatgaccc cgccggtaaa aatggtgatc ggaaagctcg tgatcagctt ggaaggcggc
     5461 tgagcgaagg tctcctgggc gacaagagat tgcttcatga aagtgagtgc agtcgcgaga
     5521 acaagcagga agcggccccc gcggcagggc tgccagtgcc gcgcattttg gcgtttttgg
     5581 ttaagcatcg tctagctttg gtccttaaat atatcccata ttttggtcgc atcaaagtac
     5641 atttgcggaa gaatccacga acggcaatct tgcgcctatc aacagcagtc atgagtctat
     5701 caacatttta cgagaaagca ttgaggagag agttcctcga catcgaagaa ggcgtcaccc
     5761 ttttccgcga agcccctttg acggagctga tggcggtggc ggacgaattg aggaaatcgg
     5821 ccgtgcctca cggaaaggtc acctggcaga tcgaccggaa tgtcaatacg accaatgtct
     5881 gtatcgccaa ctgcaagttt tgcaatttct atcgcatacc cgggcatgcc gatgcctata
     5941 tcacggatat cgacacctat aaaagaaaga tagaggagac ctttcgctgg ggcggagacc
     6001 agctgctcct ccagggcggt catcatcccg aattgggtct cagcttttat acgaatcttt
     6061 tccgccagtt gaaagagcta taccccgccc ttaagctgca cacgctgggg ccacccgaag
     6121 tggcgcatat ctgtaagttg gaaaagatga gccaccatgc cgttctttcc gcgctacgca
     6181 aagcaggaat ggactcgtta ccgggggccg gcgcggagat actcgtcgac cgggtacgcc
     6241 gtctgatcag caagggaaaa tgcggcgccc gggagtggct cgacatcatg cacgaggcgc
     6301 acaacctgca tatcaccacg tccgcgacca tgatgttcgg gcatgtggag accctgcatg
     6361 aaagaatgga acatttggta aagatccgcg aggtacaatc ccgaaaaccg gaaaacgcca
     6421 aaggattcct ggcttttatc ccctggacct tccaggacgt ggataccctg ctgacacgca
     6481 tccgcggcgt ccacaacctg accaccccgg aagaatatat ccgtatgatc gcgatcagcc
     6541 gtatcatgct gccgaacgtg aaaaatattc aggcttcctg gctgaccgtg ggtaaacaga
     6601 cggcccagct atgtctgcat gccggggcca acgatttcgg gagtatcatg ctggaagaaa
     6661 atgtcgtcag cgccgcgggc gccccgcacc gtttcacggc aaagggtatc caggaggcga
     6721 tccgcggggc cggtttcgag ccccagttac gcaaccagca atacgagtgg cgttcattgc
     6781 ccgacgttat ggaagaacag gttataaact attaatagcc agccactaag tatttacccc
     6841 gatttttttg tagtcccgga aacttaaacg ggggattttt cgttcctatt aggtagaatt
     6901 cctgttagac cggaaccgct tccagcggtt cgaccgaagg tccgaaatta gcctgttgcg
     6961 cgaatgaggc ttattttcgc tttaaagccg aattcgcgca acattattca tgaaaagccc
     7021 ttttcgcaaa ttagtccgaa tttcggtcgt cttattggta ttggtgctct tattcaattt
     7081 cttcggatat tatatcaacc gtataagatc cctggaaaac aaggaattaa ttgaagctat
     7141 caaccggtcg ggccacctgc agaccctcag ccagagcatt gccaaggagt cgatcctgct
     7201 attgaacact tctgaccaac ggacggcctc catggtcagg gacacgcttg gcaggctgat
     7261 acctgaattc cagggtagcc aggcattact gcagcaacag gccggatcca cgccttcccc
     7321 cgtgccgcag cagatattcc agataaaatt attgctctcc acctctcagc cttttttcga
     7381 ggccgtgacc gccatcgggc aggagctgac gcagtcggac tcattgctgc tggccatgaa
     7441 taaaaagctc tatctgcgca acctgttgta caacgaagcg aaatacaact cgctgatggg
     7501 ggagatcaac cagcattatt cgacgctggt aaacgagact aacggagaag tagcgaccat
     7561 cgataccggt aagctgatct cgctgatcgc cgctatcctc ggtctgatca tcctcgttct
     7621 cgaacctgcc ttcaaaaaag gcgaaaaaaa ttataacgag cttcaaaaag caaaaaatga
     7681 cctgctccac gagagcaaat tcctggcatc gatcctgcgg tctcaaacca actatgtcat
     7741 ccggatcaac cgcgccggtc aattcaccta tgcaaatcct gcctttctga agaccttcgg
     7801 gtatactgag gaagagatcc agcatatcct gttctatacg accattttcc ccaaggacat
     7861 ggcccgatgc cagcaggtgg cgagtgaatg ctggaacaat cccggaaagg tctcgcgtct
     7921 gctgatccgc aaacccatcg gacgaagccg cgaat
//
LOCUS       DASYSV010000325         5698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_63810, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000325 DASYSV010000000
VERSION     DASYSV010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63810"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VGQ51_09685"
     CDS             complement(<1..788)
                     /locus_tag="VGQ51_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036883.1"
                     /translation="MGNYRILLKVVGLSLCILINSRLTVKAQCSGTLATHTYDTALSS
                     NGYGIYPLNIPQWSPDSGLLVSVKLSANVSSQYGYTLRNATSQAASYALTLGQLDQFN
                     SPALSTPYSNVTQQYIDSFALTPGQSVSNGPFAFLNNHVSSDSITSNVTPFMGAGRVS
                     VKYTSFTFTDLNSYNNATYYYAANIANTMHFTVSYLYCQDVNVGVLAVKLSNWDAQLV
                     GTGSADLKWTAANETAGRTYDIQRSGDGQKFMTIHTVAALNGQT"
     gene            complement(1032..2171)
                     /locus_tag="VGQ51_09690"
     CDS             complement(1032..2171)
                     /locus_tag="VGQ51_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9036884.1"
                     /translation="MNKIYTLSLSGLMLITAPSFAQYTAIRAGDWHNTGAPSNTIWAG
                     AEPPASCGAGCTITLAVVGGGTINLNKHVVFSAGAQMTIGDGTNANATVLEIANSSAT
                     DTGHANSITMINDNTGTVINVTAASSLVVAPNSGNQGDFDGVFTAYVTPGTPPSESFF
                     KSVGYAPNGIVNNTIVSGQTPSLQELTGGQTLNSAGTLPILLSAFGATLGEGGVDLDW
                     TTSLEINSDHFVVERSSNAGAGWDVVGTISAHGNSVATLHYSFTDNKPVQGTSEYRLQ
                     MVDKDGKYAFSEVKTIRTGMITGVSVYPNPARDFVNVTLGSASGFTLIRLYNQGGQLL
                     QEKSTNNPGGSVIPLSVSGYPEGNYIVVVTGADGTRQANKVVITK"
     gene            complement(2568..3308)
                     /gene="rnc"
                     /locus_tag="VGQ51_09695"
     CDS             complement(2568..3308)
                     /gene="rnc"
                     /locus_tag="VGQ51_09695"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV9036885.1"
                     /translation="MGILERIIKGDAAVTSFKKQLRNVLGFSPGKAVLYRTALTHRSV
                     KDGADENNERLEYLGDAILSAIIADFLFKKYPYKEEGFLTEMRSKMVNRNQLNEIAIK
                     MGLKKISNFNKFDSSLKMSQIFGNTLEAIVGAIYLDKGFEKCRQWVMERVIQPYLFLD
                     DLENLEINHKNKLYGWANRNGKNLEFETLDERIEGGRRLFTVGAVVDGELISQGKAYN
                     KKDASQIAAQLALDKLGLNKIDSGEEGA"
     gene            complement(3378..4631)
                     /gene="fabF"
                     /locus_tag="VGQ51_09700"
     CDS             complement(3378..4631)
                     /gene="fabF"
                     /locus_tag="VGQ51_09700"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV9036886.1"
                     /translation="MQLKRVVVTGIGALTPIGNNTPDYWKGLLDGVSGAAAITLFDAS
                     KFKTKFACELKGFDPLNYLEKKEARKVDRYTQIALAASDEAVRDAGFDKEKMDPDRIG
                     VVFGSGIGGLITFQEEVINFAKGDGTPRFNPFFIPKMILDIAAGHISMRHGFRGPNFA
                     VTSACASSTNAIMEAYNLIRLGKADVIISGGSEAVVSEAGVGGFNAMKALSERNDDPS
                     TASRPYDKDRDGFVMGEAAGVLVVEELEHAKARGAKIYCEILGCGATADAYHMTAPHP
                     EGLGAKNVMMAALKDAGLTPADIDYINTHGTSTPLGDTAEIKAIMHVFGEQALSLNIS
                     STKSMTGHCLGAAGVIEAIACVMAVVNDQVPPTINHFTDDPELDPRVNFTFGRSQPRT
                     VRAALSNTFGFGGHNACVIVGKYEG"
     gene            complement(4845..5081)
                     /locus_tag="VGQ51_09705"
     CDS             complement(4845..5081)
                     /locus_tag="VGQ51_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV9036887.1"
                     /translation="MSDIATRVKKIIVDKLGVEEAEVTNEASFTNDLGADSLDTVELI
                     MEFEKEFNISIPDEQAETITTVGQAIAYLEEHAK"
     gene            5399..>5698
                     /locus_tag="VGQ51_09710"
     CDS             5399..>5698
                     /locus_tag="VGQ51_09710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9036888.1"
                     /translation="MASENYTNRMALRMIDHGTKEYQQMVHLRNEMLRKPLGLYFTRE
                     ELDLEKDDILMGAFEDDRLLGCCLLTRQDAATVRLRQMAVPSGMQGKGIGRALTIF"
BASE COUNT         1294 a         1637 c         1407 g         1360 t
ORIGIN      
        1 tccgtctgtc cattcagcgc cgcaacggta tggatcgtca tgaacttctg accgtccccg
       61 ctacgctgga tgtcgtaagt ccttccggcc gtttcattcg cggccgtcca cttgaggtcg
      121 gccgatccgg tgccaaccag ctgagcatcc cagttagaca gcttcaccgc cagtacgccg
      181 acattgacat cctggcaata gagatagctg accgtgaaat gcatggtatt ggcgatattg
      241 gcggcatagt aataggtggc gttgttgtag ctgttcaggt ccgtaaacgt aaaagacgta
      301 tacttcacgc tgacccggcc cgcacccatg aaaggcgtca cattgctggt gatgctgtcg
      361 ctggagacat gattgttcaa aaaggcgaaa ggcccgttgc tcaccgattg accgggagta
      421 agggcaaagc tgtcgatgta ctgttgcgtc acattactgt acggggtgga aagcgcggga
      481 ctgttgaact ggtccagctg acccagcgtc agcgcatagg acgccgcttg cgaggtggca
      541 ttacgaagcg tatatccgta ttgggaactc acattcgcgg acagcttcac ggagaccaat
      601 aaacccgaat cgggactcca ctggggaata ttgaggggat aaatgccgta tccattgctg
      661 gaaagcgcgg tatcataggt gtgggtcgca agagtccccg aacactgtgc tttgacggtc
      721 aaccgggagt tgatcagtat gcacaatgaa aggcccacaa cttttaacag gatgcgatag
      781 tttcccatca ggataatcgg acattttgga ttctagccta ttattaactc acggatgcac
      841 tcgattagta tgtggcactc agccgatctg cgcatctatg ccaaggtacc ccaataaacg
      901 cgccgttggc atggtttaaa gaccccaaaa tgggaaatta ggggcattta cttacccacg
      961 atgggaaatt tgggaaaaaa aacccctcct gatcaggagg ggtcatgctc atcgtcgccg
     1021 atggccataa tttattttgt aataacgacc ttgttcgctt gtctggttcc atcggcgccc
     1081 gtcactacta cgatataatt gccttcggga tagccgctca ccgacaaggg aatgaccgac
     1141 cctccgggat tattagtgct cttttcctgc aacagctggc cgccctggtt gtagaggcgt
     1201 ataagtgtaa accctgatgc cgatccaagg gtaacattta caaaatcgcg ggcgggattg
     1261 ggatatacgc tcacgccggt gatcatgccc gtacggatcg tcttaacctc ggaaaaggca
     1321 tatttcccgt ctttgtccac catctgcagc cggtattcgg aggtgccctg aacgggcttg
     1381 ttgtcggtga aagaatagtg gagcgtagcc acggaattgc catgcgcgga tatcgttccg
     1441 actacatccc agccggcgcc ggcattcgag ctacgttcga cgacaaaatg gtcggaattg
     1501 atctccagcg atgtcgtcca gtccagatcg acgccgcctt cgcccagcgt ggcgccaaag
     1561 gcagacaaca ggatgggaag ggttccagcc gaattcagcg tctgaccacc ggtcaactcc
     1621 tggagactgg gtgtctgacc gctcacgatc gtattgttga cgataccatt gggcgcataa
     1681 cccaccgatt taaaaaagct ctcgctcggg ggagtgcccg gggtgacata ggcggtgaaa
     1741 acaccgtcga aatctccctg gttgccgcta ttgggagcaa cgaccagcga agaagcggcg
     1801 gttacattga tcaccgttcc ggtattgtca ttgatcatcg taatgctgtt ggcatggccg
     1861 gtgtcggtag cggaggaatt ggcgatttcc aggacggtcg cattggcatt ggtaccgtcg
     1921 ccgatcgtca tttgtgcgcc ggcgctgaat acgacatgct tgttcagatt gatcgttccg
     1981 ccgcctacga ccgcgagcgt gatcgtacaa ccggcgccgc aactggcagg aggttcagcc
     2041 ccagcccaga tcgtattgct tggggctccg gtattatgcc aatctccggc acggattgcg
     2101 gtgtactggg cgaatgaagg tgctgtaatc agcataaggc cggataacga aagggtgtaa
     2161 atcttgttca tgttcacaaa ggttaagtaa ttgaatgtgg ttcgcttcgg tggctcacca
     2221 tagacaataa aggatttacg gtccgaataa aggatttaca cccgggatgt ccgagaagag
     2281 aacggtttac aaaaaaggag attgggaaat aaaaactgcg agtgagaatg taaaactaga
     2341 aaaaatcctt ctgggatgca agcaaaatgc ccgaaaattt tcgttaacgg caatccgtgg
     2401 cttcttttcg tcgctccggt ggcccggcca tccatcttcc accttcgccc ggcggtcccg
     2461 ccacccattt tccgcccttt gcccggcggc ctcgccatcc atcttccatc cttcgcccgg
     2521 cggcctcgcc acccatcttc catccttcgc ccgatcggcg ccacccctca ggccccctcc
     2581 tcaccagaat ctatcttgtt gaggcccaat ttgtccagag ccaactgggc ggctatctgg
     2641 ctggcgtctt ttttattgta ggccttgccc tgcgatatca attctccgtc cacgacggcc
     2701 cccaccgtaa aaagccggcg accgccttcg atacgctcat ccagcgtctc gaactcgaga
     2761 ttcttaccgt tacggttggc ccagccgtat agcttgttct tatgattgat ctccaggttc
     2821 tccaggtcat cgagaaaaag atagggctgg atcacccgct ccatgaccca ttgccggcat
     2881 ttctcaaaac ccttgtcgag atagatcgcg cccacaatgg cttccagcgt attcccgaag
     2941 atctggctca tcttcagact gctgtcgaat ttgttgaaat tgctgatctt tttgagaccc
     3001 atcttgatgg ctatctcatt cagctggttg cggttcacca tcttgctccg catttcggtc
     3061 agaaaacctt cctccttgta tggatatttc ttgaaaagga aatcggcgat gatagcgctg
     3121 agaattgcgt ccccgaggta ttccagccgc tcgttgtttt catcggcgcc gtccttgacg
     3181 gacctgtgag tgagtgccgt cctgtataag acggccttgc ccggcgaaaa tccgagtacg
     3241 tttcgaagtt gctttttgaa cgaagtgacg gcggcgtcgc cttttattat acgttccaga
     3301 attcccacct caacaagcta caattttttt cacccaataa aaaatttgtt tgacgcagca
     3361 accagcacca cgctcggtta cccttcgtat ttaccaacga tgacacaggc attatgtccg
     3421 ccgaagccaa acgtattgct cagcgccgcc cgcaccgttc tcggttgaga tctcccgaaa
     3481 gtgaagttga ccctcgggtc cagctcgggg tcgtcggtga aatgattgat ggtcggcggt
     3541 acctggtcat tcacgacagc catcacacac gcgatggcct cgatgacgcc cgccgcgcca
     3601 agacaatgcc ccgtcatgga tttagtgctg ctgatattga gagataaggc ctgctcgccg
     3661 aaaacatgca taatagcttt tatctcggct gtatcgccta aaggagtaga ggtcccatgc
     3721 gtattgatat aatcgatgtc cgcaggcgtc aaacccgcgt ctttcaaagc cgccatcatt
     3781 acgttcttgg cgcccagccc ttccggatgc ggggccgtca tatgataggc atcggcagtc
     3841 gctccgcaac ccagtatctc gcaatagatc ttcgcgccgc gcgccttcgc atgctccagc
     3901 tcctcgacca ccagcacccc ggcggcctcg cccatcacaa acccgtccct gtccttgtca
     3961 taaggacgac tggcagtgga aggatcgtca ttacgctcgc tcaacgcttt catggcattg
     4021 aagcctccca cacccgcctc gcttaccacc gcctcactgc caccgctgat gatcacatcg
     4081 gccttcccca gacggatcag attgtaggcc tccataatgg cattcgtgga agaagcacat
     4141 gcgctggtca cggcgaaatt aggaccgcga aatccatgcc gcatcgaaat atgacccgcc
     4201 gcaatatcga ggatcatctt tggaatgaag aaagggttga aacgcggagt cccatcgccc
     4261 ttcgcaaaat tgatcacctc ttcctggaag gtgatcagcc cgccgatacc gctgccgaat
     4321 acgacgccga tccggtccgg atccatcttt tccttgtcga aacccgcatc ccttaccgcc
     4381 tcgtcactcg ccgccagcgc gatctgagta taacgatcca ccttgcgcgc ttcctttttt
     4441 tccaggtaat tcaagggatc gaatcctttc agctcacacg cgaacttagt cttgaatttt
     4501 gacgcatcga acagggtaat ggcagcagca ccggacacgc catccagcaa acccttccag
     4561 taatccggcg tattgttacc aatgggagtg agggcaccga tgcccgtaac tactactctt
     4621 ttcaattgca taatggactt ttatctatcc ttcgacctgc cccgttcagt ggctgtttat
     4681 ctatccttca cccctacccg ttcagcggct gtttatctat ccttcaacct gccccgttca
     4741 gcggctgctg gctaaggata aaatccgcct tctaagccca attgcttgaa aaggcggatc
     4801 gaacgagttt attacaataa ctccaaacga agttttgagc gctcttattt agcgtgctct
     4861 tccaggtaag cgatagcctg accgacggtg gtgatcgtct cggcttgttc atcgggaatg
     4921 gaaatgttga attccttctc gaattccatg atgagctcga cggtgtccag agagtctgcc
     4981 cccagatcgt tggtgaaaga agcctcgttg gtaacctctg cctcctctac tcctaactta
     5041 tcgacgatga tcttctttac tcttgtagcg atgtctgaca ttttggtaaa tttttagtta
     5101 tacgcccgca aaaataaagg ttccggacta ctgtacaaaa ttgaggcatt ttattttaac
     5161 ctcccggcct ccgtaaaagc ccgcccatgc cacagcacca gccaaccctt cccaccttcc
     5221 atccccgcca atgccacggt gccagccaac ccatccgacc ttccatcccc gcccatgcca
     5281 cagtaccagc cgacccatcc caccctccga acctccgggg gaccgtagcc gaagcccccc
     5341 gttttacggt aatttttccg tattttcggg cctcccggcc atcggcacat gccgccgaat
     5401 ggccagcgaa aattatacca accgcatggc gcttaggatg atagatcacg gaacgaaaga
     5461 ataccagcag atggtccatc tgcgcaacga gatgctgcgc aaaccattgg gtctttattt
     5521 caccagggaa gagctcgatc tggaaaagga cgatatcctt atgggcgcct ttgaagacga
     5581 ccgcctgctg ggctgctgcc tgctcacccg acaggatgcc gccactgtcc gtctccgcca
     5641 aatggcagtc cccagcggta tgcagggcaa aggtatcgga cgggccctca cgatcttc
//
LOCUS       DASYSV010000326        10372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_576307, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000326 DASYSV010000000
VERSION     DASYSV010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576307"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VGQ51_09715"
     CDS             complement(<1..915)
                     /locus_tag="VGQ51_09715"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEV9036889.1"
                     /translation="MNRKKWFLLMLLAGGQTAFAQNSGDTTNSRQLDEVVVTATKYPV
                     KQSLTGKVLDVITRDQLDRNEGRPLTEILNEQAGVVIIGAQNTLGSEQDVFMQGGHSG
                     TTLILIDGVPAYDPSGITTNFDLNLMNTDEIERVEILKGSQSTLYGSDAMAGVINIIT
                     KKGNGKPFNAALNTSFGSYNTFRGSAGVDGRIGSTAYNVQYTKLRSDGMSDAYDSSGK
                     GNFDKDGFNEDVVMVNVNQQVSDVLQLRGNFQYSRYSHGLDAGGYTDDPNYNTGSRNT
                     QAGIGADYKLGTAMLHFNYNYNTVTRNYL"
     regulatory      complement(1010..1222)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1230..2228)
                     /locus_tag="VGQ51_09720"
     CDS             complement(1230..2228)
                     /locus_tag="VGQ51_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9036890.1"
                     /translation="MSSPLLYAKNLSTGYHSGGRKIVVSAGLPELEIDRGRMICLLGA
                     NGSGKSTLLRTLAGLQPALGGTVAVGGATHLSPADLARRISLVLTDRVVGTNLDVYSL
                     VVLGRYPWSGWLGGLDAADRSAVGRAMDAAGIMDLQHRKVHTLSDGESQKVMLARALA
                     QDTPLLMLDEPTAHLDLPSRIRLMRLLHTLTREMNKGILLSTHELDLALQVADELWLL
                     QSGGVLHKGAPEDMVLEGAFEAAFAGEDVVFDRATGVFRIHPEGGPAIRLEGDGTLAF
                     WTRRALQREGFTVIREETADRVVRIIEEKSAPRWVLETAGASRSYTSIGALLRELK"
     gene            complement(2225..3277)
                     /locus_tag="VGQ51_09725"
     CDS             complement(2225..3277)
                     /locus_tag="VGQ51_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV9036891.1"
                     /translation="MNCSIRKLSLTGLLLLLVLACVLDISAGAVRIPLHDIFRILFSR
                     APAADASWSFIVDKIRLPKAITAILAGCGLSVGGLQMQTLFRNPLADPGVLGITAGAS
                     LGVAAVMLTGGSMTTIYAIKELGPSGSWLIACAACVGAGMAMLLVLVLSGRLRDNVMM
                     LIIGLMIGSVTLALISIWQYFSNPDEVKDYLLWTFGSLGGVTGKQLSILGLIVGVGLI
                     AAWLSSKMLDALLLGENYARSMGLTVRRARTIIVCTTSLLAGVITAFCGPIGFIGIAV
                     PHLARLCFNTSSHRILIPACCLIGAILLLLCDCISQWPGTQAVLPINIITSLVGAPLV
                     ILLLVRRRNLSINAYR"
     gene            complement(3274..4410)
                     /locus_tag="VGQ51_09730"
     CDS             complement(3274..4410)
                     /locus_tag="VGQ51_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV9036892.1"
                     /translation="MFRFLFILVLLFPAGCRQPSTAKPADRPVGVVDTIHYAHGFFIV
                     RYPGYRDVGIIDRRAGRADTLHFLLVEQGVAPPADRSGLPVIVTPVKSMVVLSSMHVA
                     MAEFAGVADRITGLGSIQYVYSPIVRAGIRAGKVKQVGIDAGINNEAVISLHPDLVLT
                     MSNPQAGAGAYKTLADAGIPVIPDADWLETSPLGRAEWVRLWGALVDKDAMVNKKFDS
                     LVQSYQRLAAIGSQASPKPTVITDMPFKGTWYVPAGESFMAQFLRDAGADYHWSDTKG
                     TGSLPLSFEAVAPIALKADFWLNQGLVDSRADVGAKDSRFASFKSFQTGNLYNYNRRI
                     NDLGSNDYWESGSMHPDLILADLIRILHPGLLPQDSLYCYKQLK"
     gene            complement(4477..4944)
                     /locus_tag="VGQ51_09735"
     CDS             complement(4477..4944)
                     /locus_tag="VGQ51_09735"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEV9036893.1"
                     /translation="MAECWTINGVLLYGRSLFKFNMSPIPAGAIINSAKLVLFSDTLP
                     DNGDLIHANYGTTNDFWIQRVAGAWDQNTNFNTLPSLDTAGEVHVPQTSQPFLNLNLD
                     VTTMVNNMATNGNNGFEMKLNTEQLYNSRIFCSSLYSDTTRRPYLSVTYTVHP"
     gene            5382..6008
                     /locus_tag="VGQ51_09740"
     CDS             5382..6008
                     /locus_tag="VGQ51_09740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV9036894.1"
                     /translation="MLSSWVNKMSRTTTNSDFYTPLRKYTRRINLHESVIKSQGLADR
                     PIHYLEFGVAGGTSFMWWVKANHHPDSRFFGFDTFEGLPEDWHFFKKGAFSFDIPFLE
                     DERGTFIKGLFQDTLYKFLDNYRNSKDTPDTTRVLHMDADLYSSTLFALTMMAPFLRN
                     GDIVLFDEFNIPNHEFAAWSDFIKAYYIKYEVIGSVNNFYQTAIRIIK"
     gene            6052..6828
                     /locus_tag="VGQ51_09745"
     CDS             6052..6828
                     /locus_tag="VGQ51_09745"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEV9036895.1"
                     /translation="MKIKQLLLSILGEKRYLFLLSNTFQVLCRMGFLGKEYDDILFLK
                     RYIRKGDACLDIGAHLGYFTIELSRLTGPEGKVFAVEPMSKFNRVLERLLRKYKADNV
                     TLYRVALGGNGDYVEMGIPEIGRAKKFAYARVMDSQPGLRYVDTERVENHQGDSLFGD
                     LKRLDFLKIDVEGLEFSVLSSMMHTITTHRPILLCEFFEGEQRLKLYHLLEPLGYRAY
                     RLDKDTWHEIDVFGEGDAITQNNYFIAAGQRERLGYLFTP"
     gene            complement(6810..8903)
                     /locus_tag="VGQ51_09750"
     CDS             complement(6810..8903)
                     /locus_tag="VGQ51_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9036896.1"
                     /translation="MKKNHLLLLMACGHTLFSFGQQHHPSDVLADSSDTRPLNEVVIK
                     AYEQNRKLADVGAPVNIVNKTSLDRFANTSILPAMNSNPGVRMEERSPGSYRLNIRGS
                     SLRSPFGVRDIKIYWNEIPLTDPGGNTYLNELGFYNFQSLEIIKGTAGSLYGAGIGGA
                     VLIRSQPAVWDAGVRADYTRGSYNTNNSNIDVRSGDADHRNIFAYSHQTSDGYRQQTQ
                     MRRDVGSWETQIKASDKQTIHAYVLYSDLYYQTPGGLNHTEYNKDPRMARPPVGGQPG
                     AVQAQAAIFQKNFTAGFANDHQFSEHWQNVTSVYGAYTDFTNPGIRVYEYRQEPHFGG
                     RTVFQYKTTLGIGAFQLNAGAEGQRGFFETRDYANRSGVEDTLQTDDRLSNWTWMVFA
                     QGDLRLHGGWTLTAGASFNENAVNITRVSVVPPVTHPVRFDNKLAPRISLLKKITADL
                     SVYASAARGFSTPTVQELEKSNGVVGPPLQPEDGIDYELGLRGSLLKGRLFYDIDAFV
                     FHIRNAIVQRIDSFGVGYSVNAGGTDQRGLESLVSYRIIDEPGSFFNLLRVFISDTWH
                     DFHYSSFMQDTNNYSHHRLPSVPPQLVVMGLDIASRLGVYANLTYTYSDHVALNDANT
                     AWAGSYNLMGGRIGYRLTAGGRFRLNAYTGIDNAFNVKYSLGNDFNAAAGRYYNVSPG
                     RNYFAGVSLDYGVKR"
     gene            9036..10364
                     /locus_tag="VGQ51_09755"
     CDS             9036..10364
                     /locus_tag="VGQ51_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV9036897.1"
                     /translation="MSKKQLNLFDLSMIVVSLVIGMGIFSTPVVAANRAGTPSIFFLA
                     WGLGGIIALCGALTYAEIGSRYPVTGGYYKIFSLAYHPSLAFSINCIELVSNAGSTAF
                     VAVVGAEYIGRFFYPAGPTPAIFRGGVTAVAVALLYIINLLGLRMSSRVQNTLTLIKI
                     LLIFLLLASLFGSHPLPAATSARMAAPEHPLGFFRALGFALVAVSFSYGGYQQTINFG
                     GEIRNPGSIIPRGIGIGIAIIISLYLSLNFVYTEVIGFQSLKTANSIGALLMGHLFGP
                     AGDAILRCLIFISVLAYVNVSIMSNPRVMFAMSGEGILPAVFGKRTARHEVIIWSLTT
                     FCATIILTLLFSTQVEKIINWTIFMDSIAFAFSASTLFVLRRRRMGEDREIYKMKGYP
                     WITGFFVLAYLSVTTSIAISDPQSAFYCFLLFCGFFILYFILKWTSQGTR"
BASE COUNT         2267 a         2897 c         2747 g         2461 t
ORIGIN      
        1 caggtaattc ctggtcaccg tattgtaatt gtaattgaaa tggagcatgg ccgttcccag
       61 cttgtagtcg gcgccgatac cggcctgtgt gttcctgctg ccggtgttat aattcggatc
      121 gtcggtatat ccgcccgcgt ccagtccatg gctataccgg ctgtattgga aattgccccg
      181 cagctggaga acgtcgctca cctgctggtt gacatttacc atgacgacgt cctcgttgaa
      241 accgtccttg tcgaaattgc ctttgccgga ggagtcatag gcatccgaca tcccgtcgct
      301 tcgcaatttt gtatactgta cgttataggc cgtcgagccg atcctgccat cgacgcccgc
      361 cgatcccctg aaggtattat agcttccgaa cgatgtgttg agagcggcgt tgaacggctt
      421 cccgttacct tttttggtga tgatgttgat gacgccggcc attgcatcgc tgccatacag
      481 cgtggactgg cttcccttga ggatctctac cctctcgatc tcgtcggtat tcatcagatt
      541 gagatcgaaa ttggtcgtga tgcccgatgg atcgtaggca gggacgccgt cgatgaggat
      601 cagtgtcgtt cccgaatgcc cgccttgcat gaagacatcc tgctccgacc ctaaagtatt
      661 ctgtgcgccg atgatgacca cccccgcctg ttcgttgagg atctccgtaa gggggcgccc
      721 ctcattcctg tcgagctgat cgcgcgtgat cacatccagt actttgcctg tcagcgattg
      781 tttcacgggg tacttggtag ccgttacgac tacttcatcc agttgccgac tgttggtagt
      841 gtctcctgaa ttttgtgcga atgccgtctg gccgccagcc agcagcatta agaggaacca
      901 ttttttcctg ttcatgtccg ataattttgt ttggcggctt gctgattttt gagagagggg
      961 aggctttgag aatggccttc ccattctcgc ttttcacccg aaagccttga atgttgataa
     1021 tagatcaggc aggtcttctg gcttatgctt caccggctca ccttcccatc cgcgtttgcg
     1081 gacagtggtt atggaggatg ccggtgattg ccgccgccgg aatggcgaca ggcagcattt
     1141 acagctacgg ggatagcgcc ggatttacac cggtcttccc ttttaatgac gcgaggcgtc
     1201 aaccatgatc gcggtaaagg tagtgtattt tatttaagct cgcgcagcag tgcgccgatg
     1261 gaagtatagg accggctggc gcccgcagtc tccagcaccc atcgcggcgc acttttttct
     1321 tcgatgatgc ggaccacgcg atccgctgtc tcttctctta tgacagtgaa tccttctctt
     1381 tgtagcgccc tgcgcgtcca gaaagcgagt gtgccgtcgc cctccagtct tatggccggt
     1441 cccccctcgg gatgtatgcg aaacacccca gtcgccctat cgaacacgac atcctcgccc
     1501 gcaaacgccg cttcaaaagc tccctcgaga accatatcct ccggtgcgcc cttatgcagg
     1561 acgccgccgc tctgcaacag ccataattca tcggcgacct gcaatgccag gtccagctca
     1621 tgcgtggata gcaggatacc cttattcatt tcgcgggtca gcgtatgcaa cagacgcatc
     1681 agccggatac ggctcggcag gtctaaatgc gcggtgggct catccagcat cagcaacgga
     1741 gtatcctggg ccaacgccct tgccagcatc accttttggc tttctccgtc tgaaagcgta
     1801 tgcaccttcc ggtgctgaag atccatgatg cccgctgcat ccatggcccg cccgacggcc
     1861 gaccggtcgg cggcatcgag tccgcccaac catcccgacc agggatatct ccccagcaca
     1921 accagcgaat acacgtcgag gttagtaccc acgacccgat ccgtcaggac gagacttatc
     1981 cttcgcgcca ggtctgcggg ggagaggtgg gtggcgcccc ctactgcaac cgttcccccc
     2041 agcgccggct ggagcccggc aagcgtcctt agcagggtcg attttcccga gccgttagcc
     2101 cccagcagac agatcattct cccgcggtct atctccagct cgggcagtcc ggcagagacg
     2161 acgatcttcc gtcctccgct atggtaccct gtcgaaaggt tcttcgcgta tagcaatgga
     2221 ctgctcatcg gtaagcgttt atggaaaggt tccgtctccg caccagcagg aggatcacca
     2281 gcggcgcgcc caccagcgaa gtaatgatat tgataggcag caccgcctgc gttcccggcc
     2341 actgagagat acagtcgcac agcaacagca aaatggcgcc gatcaggcaa caggcgggga
     2401 tcaggatgcg atggctggag gtgttgaagc acagcctcgc caggtgcggc acggcgatgc
     2461 cgataaatcc gatcgggccg cagaaggcgg taatgacacc tgccaggaga ctggttgtgc
     2521 agacgatgat cgtacgcgcg cgccggaccg tgagtcccat acttctggca tagttttcac
     2581 ccagcagcaa cgcgtccagc atcttggaag aaagccaggc ggcgatgagc cccaccccga
     2641 ctatcagccc caggatcgac agctgttttc ccgtcactcc gcccagcgag ccgaaggtcc
     2701 ataacaggta gtcttttact tcgtcggggt tgctgaaata ttgccagatg ctgatcagcg
     2761 ccagcgtgac gctaccgatc atgagcccga tgatgagcat catgacattg tccctcagtc
     2821 tgcccgaaag gacaagcacc agcaacatcg ccatcccggc gcccacacag gcggcgcagg
     2881 cgatcagcca gcttccggac ggccccagct cctttatggc atagatggta gtcatgctgc
     2941 cgcccgtgag catgacggcc gctacaccga gcgaagcgcc agcggtgatc cccagaacac
     3001 cggggtcggc caacgggttg cggaagaggg tctgcatctg aagaccgccc accgagagcc
     3061 cgcaacccgc aaggatggcg gtaatggcct ttggcagcct gatcttatct acgataaagc
     3121 tccaggaggc atcggccgcc ggggcccggg aaaaaagtat ccggaagata tcgtgaaggg
     3181 gtatacgtac tgcacccgcc gagatgtcca gcacacaggc cagcaccagc aaaagcagca
     3241 aacccgtaag cgacaatttc cgtatagaac agttcatttc agttgtttat aacaatataa
     3301 actgtcttgt ggcagcagac ccggatgcaa gatccgtatc agatcggcca ggatcagatc
     3361 gggatgcata gaacccgatt cccagtaatc gttcgaccca aggtcattga tacgccggtt
     3421 gtaattatat aggtttccgg tctgaaagga tttgaaactg gcaaaccggc tgtctttcgc
     3481 gccgacatcc gccctggaat cgaccagacc ctggttcagc caaaaatctg ctttcagcgc
     3541 aatcggcgcg acggcttcga atgatagggg caggctgccc gttcctttgg tgtccgacca
     3601 gtgataatcg gcgcccgcat ctctcaggaa ttgcgccatg aaactttctc ctgcgggtac
     3661 ataccaggtc cctttgaagg gcatgtccgt aataacggtt ggcttcgggc tggcttggct
     3721 cccgatggct gccagacgct gatacgactg caccaggcta tcgaatttct tattcaccat
     3781 cgcgtcctta tccaccagcg caccccacag cctgacccat tcggcacgcc cgagcggcga
     3841 cgtctccagc cagtctgcat caggaatgac gggtatcccg gcatcggcga gggtcttgta
     3901 agccccggcc ccggcctggg gattactcat cgtgagcaca agatcgggat gaagcgagat
     3961 caccgcttca ttgttgatcc ccgcatcgat ccccacttgt ttcacctttc ccgcccggat
     4021 acccgcgcgg acgatggggg agtagacata ttggatactg ccgagacccg ttatgcggtc
     4081 ggcgacgcca gcaaattccg ccatggcgac atgcatcgag gaaaggacaa ccatgctttt
     4141 caccggggtg acgatcaccg gtaagccgct tctgtccgcg ggcggcgcca ctccctgctc
     4201 caccagtaaa aaatgcagcg tatcggcccg gccggccctt cgatcgatga tcccgacatc
     4261 cctgtacccc gggtacctga cgataaagaa cccatgcgca taatgaatgg tatcgacgac
     4321 cccgacagga cggtccgccg gtttcgctgt tgacggttgc cggcaaccgg ccgggaataa
     4381 taacactaaa atgaaaagga agcgaaacat ggggaccaaa actacaaaaa aaatgacggc
     4441 cccacggggc cgtcattttc aatctttttc atccatctat ggatggacgg tataggtgac
     4501 ggacaaataa ggccgccgcg tcgtgtcgga ataaagcgat gagcagaata tcctcgaatt
     4561 gtagagctgt tcggtattga gcttcatctc aaaaccgtta ttgccattcg tggccatatt
     4621 gttcaccatg gtcgtaacgt cgagattcag attgaggaaa ggctggctgg tctgcggtac
     4681 gtgtacctct ccggccgtgt ccagcgaagg cagcgtattg aagttggtat tctgatccca
     4741 ggcgccagcc acccgctgga tccagaaatc attggtggtg ccatagttgg catgaatgag
     4801 gtcgccgtta tccggcaacg tatccgagaa cagcaccagc ttcgccgaat tgatgatggc
     4861 gcccgccggt atcggactca tgttgaattt aaaaagcgac cggccgtaaa gcaggacacc
     4921 gttaatggtc cagcattccg ccatcagctc gggagtacgg ttattcgcgg cgggattcga
     4981 ccggataact ggctccagat aaagcccgtg atcctgctga cggagtcgtg tgcgctgccg
     5041 ctgagccgga ccgaatctac cggtaaagtg atggtctgtg aaggacccgc attcacggtt
     5101 ggagcgggag ggaatggagg agcgagtttt tctgacagct ggaaaaggtt acaataccag
     5161 caatcgcaag gataggacga cgctaaagta gaaaaaatat ctctgcgact atagcgtttt
     5221 taggtctatc tttaagcccc ttatatttgg aaaatttatt atttaatatg agcttagtcc
     5281 gaaaagctaa ttcatttatc aaggatctgt tgttggtttc gagggtagga attcttttcg
     5341 ctccattccg cttttttttt cggtttctgt ataatttctc gatgctgtca tcctgggtga
     5401 ataaaatgag ccggacgacc acgaattcgg acttttatac tccgttgcgc aagtataccc
     5461 ggcggatcaa tctgcatgaa tccgtgatca aaagccaggg tctcgcagat aggcccatcc
     5521 attacctgga attcggggta gccggcggca cttcgtttat gtggtgggta aaggccaatc
     5581 accatcccga ttcccggttt tttggattcg acaccttcga aggtctgccc gaagactggc
     5641 atttctttaa gaaaggggca ttcagtttcg acatcccctt tttggaagac gagcgaggaa
     5701 cctttatcaa ggggttgttc caggatacgc tctacaaatt cctcgacaat taccgcaact
     5761 ccaaagatac gcccgacacg acccgcgtac tccacatgga tgcggatctc tattcttcca
     5821 cgctgttcgc gctgacgatg atggcgccat tccttcgcaa cggcgacatc gttcttttcg
     5881 acgagtttaa catccccaac catgaattcg ccgcgtggag cgattttatc aaagcttatt
     5941 atataaagta cgaagtgatc ggatcggtga acaattttta tcagacggcc atccggatca
     6001 taaaataaac cggcggcatc gctgccggta accctggcgg aaggaggtaa catgaagata
     6061 aagcaactac tgctgtccat tttgggtgaa aaaagatatc ttttcctttt atccaatact
     6121 ttccaggtcc tttgccggat gggatttttg ggtaaagaat acgatgacat cctgttcctc
     6181 aagcgatata tccggaaagg agatgcctgc ctggatatcg gcgcgcacct cggatatttc
     6241 acgatcgagc tctcgaggct gacgggtccc gaaggaaagg tctttgcggt cgaacccatg
     6301 agtaaattca accgggtact ggaacgattg ctgcgaaaat ataaggcaga caatgttacg
     6361 ctctaccggg tggccctggg cggcaacggg gattatgtcg aaatgggcat ccccgagatc
     6421 gggcgggcca agaaattcgc ctatgccagg gttatggatt cgcagccggg tctccggtat
     6481 gtcgataccg aaagggttga aaatcaccag ggcgattcgc tgttcgggga cctgaaacgg
     6541 cttgattttc taaagatcga tgtggaaggc cttgaatttt ccgtattatc gtccatgatg
     6601 cataccatca cgacccaccg ccccatcctg ttatgcgaat ttttcgaggg tgaacagcga
     6661 ttaaagctct accatctcct ggagccgttg ggatatcggg catatcgcct tgataaggac
     6721 acatggcatg agatcgatgt ctttggagaa ggcgacgcga taacccagaa caattatttt
     6781 attgccgccg gacaacgcga gcggctcggt tatcttttca ccccataatc gagagagact
     6841 cccgcaaaat agttcctgcc cggtgagaca ttgtaatatc tccctgccgc tgcattgaaa
     6901 tcgttaccga gactgtattt gacattgaac gcattgtcga taccggtata tgcattcagc
     6961 cggaatcttc cccccgccgt caaccggtag ccgatgcgtc cccccatcag gttgtaagat
     7021 ccggcccagg ccgtatttgc gtcgttgagt gcgacatggt ccgaataggt gtacgtgaga
     7081 ttcgcataca ccccgagtct cgacgcaatg tccagcccca tgacgaccag ttgtggcggc
     7141 acgctcggga gacggtgatg agaataattg ttcgtatcct gcatgaagct gctgtaatgg
     7201 aaatcatgcc aggtgtcgct gataaaaaca cgcagcaggt tgaaaaagct tccgggttca
     7261 tcgatgatcc tgtaggaaac caggctctcg agaccccgct gatccgttcc gccggcattc
     7321 accgaatagc cgacaccgaa agaatctatg cgctggacga tcgcattccg gatatggaag
     7381 acgaaggcgt cgatgtcgta gaacagccgc cccttcagca ggctaccacg taacccgagc
     7441 tcataatcga tgccatcttc cggttgcagc ggcggaccca ccacgccatt gcttttttcc
     7501 agctcttgta cggtcggtgt agagaacccg cgtgccgcgc tggcatagac cgacaggtcc
     7561 gcggtgatct tttttaacaa ggagatccgc ggggccagct tattatcgaa acgcaccggg
     7621 tgagtcacgg gaggcactac cgatacccgg gtaatattga ctgcattctc attgaagctc
     7681 gcacctgccg tcaacgtcca tccgccatgt agtcgcagat cgccctgcgc gaagaccatc
     7741 catgtccagt tgcttagccg gtcatccgtc tgcaatgtat cttcgacgcc cgatctgttg
     7801 gcgtaatcac gtgtctcgaa gaaccctctc tgtccttcgg cgcccgcatt cagctggaat
     7861 gcgccgatcc ccaacgtcgt cttgtactgg aagaccgttc ttccgccgaa atgcggctcc
     7921 tgccggtatt catatacccg gatgcccggg ttggtaaaat cggtataggc tccgtagacg
     7981 gatgttacgt tctgccagtg ttcgctgaat tggtgatcat tggcaaatcc ggccgtaaag
     8041 ttcttctgga aaatggccgc ctgcgcctgc acggccccgg gctgtcctcc tacgggagga
     8101 cgcgccatcc gggggtcttt gttgtattcc gtatggttca gaccgccggg cgtctggtaa
     8161 tagaggtcgc tatacaacac ataagcatgg atcgtttgtt tgtcggaggc tttgatctgc
     8221 gtctcccatg aaccaacgtc ccttctcatc tgcgtctgct ggcggtagcc gtcgctggtc
     8281 tgatgcgaat acgcgaagat attgcgatgg tccgcatcgc cggaacggac atcgatattc
     8341 gaattgttgg tattatagct gcctctcgta tagtctgccc tgacgcctgc gtcccacacc
     8401 gccggctggc tccggatgag cacggcgcca ccgataccgg cgccatacag actgcccgcc
     8461 gtccccttga tgatctccag cgactggaaa ttgtaaaaac ccagttcatt cagataggtg
     8521 ttaccgccgg gatccgtgag cggaatttcg ttccaatata tcttgatatc cctaacgccg
     8581 aaaggtgaac gcagcgagct cccgcggatg ttcagccggt aactgccggg tgagcgctct
     8641 tccatgcgta cgccggggtt ggaattcatc gccggtaaaa tgctggtgtt ggcaaatcgg
     8701 tccagggatg ttttgttgac gatatttacg ggagcgccga catcggccag cttccggttt
     8761 tgctcgtagg ccttgatcac gacctcgttc aaaggccgtg tatcggacga atcggcgagg
     8821 acgtcggatg gatgatgctg ctggccaaac gaaaataatg tatggccgca ggccatcaac
     8881 agcagtagat ggtttttttt catccggggt atttatctta tcaaaagtac tgtaaatacc
     8941 aaaccggacc gagtccggtt caaagacatt agcggtcaat ttcgcaaaaa attacttttt
     9001 gccaattttt tggcgaaatt caatatcttc cattcatgag caaaaagcag ctgaacctat
     9061 tcgatctcag catgatcgta gtcagcctgg tcatcggcat gggaattttc agtaccccgg
     9121 tagtagccgc gaacagagcc ggcacgccgt ccatcttttt tctcgcctgg ggcctcggcg
     9181 gtattatcgc gctgtgcggc gctcttacct acgcggagat aggctcacgg tatccagtga
     9241 ccgggggtta ttataagata ttctcgctgg cctatcatcc ttcgctggcg ttttcgatca
     9301 actgtatcga actggtatcc aacgcgggtt ccaccgcctt tgtcgccgtc gtcggagcgg
     9361 aatatatcgg gcgtttcttt tatcccgcag ggcccacacc cgcgattttt cggggagggg
     9421 taaccgcggt cgcggtagca ttactttaca tcatcaacct cctcggtttg cgcatgagca
     9481 gccgcgtgca aaatacgctg acgctgatca agatcctgct cattttttta ttgctggcat
     9541 ccctgttcgg cagccatccg ctgccggcag ccacatccgc ccggatggct gccccggaac
     9601 acccactcgg tttcttccgg gcactgggct tcgcactggt agccgttagt tttagttatg
     9661 gcggctatca gcagaccatc aatttcggcg gtgagatacg taacccgggt tcgatcattc
     9721 cccgcggcat cgggatcggc atcgccatca tcatttccct ttatctgtca cttaatttcg
     9781 tttatacgga ggtgatcggc tttcaatctc ttaagactgc caattccatc ggggcgctgc
     9841 tcatgggaca tcttttcggc cccgcgggag acgccatcct ccggtgtctg atattcattt
     9901 cggtcctcgc ctatgtcaac gtctcgatca tgagcaatcc gcgggtgatg ttcgccatga
     9961 gcggagaggg gatccttccg gcggtgtttg gaaaaaggac ggcccggcat gaggtcatta
    10021 tctggtcgct gaccacattt tgcgcgacca tcatccttac cctgctgttc agtacgcagg
    10081 tcgaaaagat catcaactgg acgatcttca tggatagcat cgccttcgcc ttttccgcct
    10141 ccacgctttt cgtactgcgc cgcaggcgca tgggggaaga ccgtgagatc tataaaatga
    10201 aaggctatcc ctggataacg ggatttttcg tcctggccta cctgtccgtt acgacgagca
    10261 tcgccatctc ggatccccag tcggcattct attgtttcct gctgttttgc ggatttttta
    10321 tactttattt tattctaaaa tggacatctc agggaacgcg gtagcgttcc gg
//
LOCUS       DASYSV010000327         8596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2367880, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000327 DASYSV010000000
VERSION     DASYSV010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367880"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VGQ51_09760"
     CDS             <1..832
                     /locus_tag="VGQ51_09760"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV9036898.1"
                     /translation="NQLFAGHLFEPGQLLDANKYFIILPDGIGHGKSSKPSDGMHMRF
                     PKYTYDDMIKADYDVLTQQMGINHLRLIIGTSMGAMHCWVWAETYPDFVDACMANASL
                     PVEIAGRNRESRYAAIKCVESDPAWKGGDYTEEPATGLRGAYTSMMWMTSSPYNMQKR
                     SPTREQSERGYDFGSENFIKHQDANDMIYAFDASRFYNPEPKLSTIKCTFYAVNSADD
                     ECNPPELGILDRDIKLIPKGRYILIPWSEKTSGHGTHSNPTVWGDYLKELMEASEPGH
                     "
     gene            complement(829..2436)
                     /locus_tag="VGQ51_09765"
     CDS             complement(829..2436)
                     /locus_tag="VGQ51_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036899.1"
                     /translation="MGWRQVIPLLFLIFSHCSLLYAQQVSYCEPYSDRFTLRQEMLGK
                     VGDFFWVSSINRRKVSRHSNDPSQERNFVIYDSRMNVINVLGDPSYVSSSIKEYLVIN
                     DDSFDRLHLLNADGKEVEVRLQRYNPDGTMAGPDHNVAVFPFNEPGNSFMLVRSQDRK
                     KILMLGFEFLPSGAPRMHAFLFDQDWQLLSQRVYRHPFLSQPMIQDDYSGFPLEDFNT
                     GDVKLANNGDWLMLAPSRNSHNFLLFHFSTVDTAVVAKEINMPGTSSMEDLCLSLDNS
                     NGEAVAGILSTFHYAPLKDVEVVHYSMNTKMFSFDSSYRLSTLAGKRVRNENIVKENF
                     VAVPGRGFLLLKEYGRPFTDEQMDEEDYDEGWDPALLFASNAIPDPSMGPSSLMRHLP
                     EPKYGYARYRATVNPPYHDRGDLSLYYFPAGRTDSCWSGMISQEQVTELNSPNLSYMI
                     VPMYNRMVFLYNSFVHGEKVYASTTVIDPGGNQVTNEGILFWGLKNTLNFQEARQVAP
                     DQVVIPYDIYINGSANGKIGFAVVLFR"
     gene            2939..5518
                     /locus_tag="VGQ51_09770"
     CDS             2939..5518
                     /locus_tag="VGQ51_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEV9036900.1"
                     /translation="MRIVLSVIFGIVLTLRLSAHPNVSTGALPKWLYDFHPHLDKTPS
                     DEDISNGYYYNLLDLQSNLACNTEYTHYVRTIVNQSGVQNASEVSVTFSPQFQQVIFH
                     HIDIIRDGAILHRLQQGRIKVVQEETDADEFQYNGLKRAFLTLEDVRKGDRIDVAFSV
                     VGFNPVFDDKYSDDFSFNSETAVCNYYKTIITTPDRPLHIRTINNAPPPVEQRIDNRL
                     VYTWNNPHMGSDDARPASNAPSWFNGYPTVYVTEYPDWPAVIGWGLQTFNQYHFSLPA
                     GLQQKISDWKTRSGGDKVAFTSLALRFVQDEVRYLGLEIGANTHRPHQPGEVFTRRFG
                     DCKDKALLLTTILQEEKIPAFVALINTDTRSRLTAVAPSPGAFDHAIVAIHRPDGKYD
                     FVDPTLTNQRGPFAEIFVPAYGYALVLREGESGLEPVTPGKINDYSIIETLDAPWYDS
                     AHFSITSVYAGGAADEIRGMFAEKSKKDLGEIYRKYYATLFDEIRQDRDITYKDDSTR
                     NMVTVGKHYSIPQVWKNGRNGQRYFDFVTKIIEQNLPDPSDAPDNIPISLPYPFNVHY
                     TLELHLPEGWAFEELHVKNDAFQFDFEPVPNGNNVSLHYSLKTFDDHISAAAVRRYKE
                     DYKNIADKISFRLSGTPGSGGSFGENSATSDTPTPSAMALSDWKACWPAIWLTFFFSL
                     FFSRLFQRLNSRSEETLYAPGSGYPLGGWLILLGISIASIIVFELIRLVQSDYYSYSS
                     WATYGNAGGVALQYLYLTQLAIQLTLICGAAALLFWFIRKRDIFPRMFLWYAGILLTG
                     RILLLVLFYAMPIPASLIGYRTIQIMALVRSGIYALIWAAYIIRSGQVKSTFLEPFRE
                     RIR"
     gene            complement(5588..6853)
                     /locus_tag="VGQ51_09775"
     CDS             complement(5588..6853)
                     /locus_tag="VGQ51_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEV9036901.1"
                     /translation="MNKITRFSMPLPVTRGGKGFLWLTRGGKGLLRLVRGGMRLSPLT
                     RGGMWLLFGGIMAVACHKSNITQKDLRNFEQVVLVANKAMYMPKLVDTTLQNGWGLAW
                     APSGIAWVNANGDHVSELYTGEGAIVRPPVNIPSPTDSVGGAPSGIVFSGGKGFVLAN
                     HQGANFLFVGEDGILSGWNGADGNNAQRIRDRSATSVYKGLALDSTGPGQNFIYAANF
                     MTGKIDVWDTGFHLVSMPFHDPALPAHYAPFNIVPVGQWLFVMYAEQTPGSIDEMHGP
                     GKGIVDVFNTDGSFVHRFASRGSLNAPWGAAWAPAGWLSMEDMSGEKGGGDDSNKGKG
                     SFGEDGKRVSEPVVLIGNFGDGRINVFSPDGEFLGQLRSHNKTIVIDGLWALGFAPAT
                     ATSIDPNRLYFTAGPADEADGIFGYLIKQ"
     gene            complement(7102..7983)
                     /locus_tag="VGQ51_09780"
     CDS             complement(7102..7983)
                     /locus_tag="VGQ51_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV9036902.1"
                     /translation="MSSRRNFLRTGLGIAGAGVAGLGVAGAGVAGLGWVDGNVKTEGG
                     GKGEIPKDLHLGIAGYTFLHVPMDQGIAMMKRLAVDAISIKDFYLPLDSGADKIAQVV
                     GAFNAAGIRIYGAGVIYMKSEADVDRAFEYAKMLGVDLIIGAPNPELLGYVEGKVKKY
                     RIRVAIHNHGPEDKLYPSPVDVYEHIKGLDDRMGLCLDIGHAARAGADIVKIVNDYAP
                     RIFDMHIKDLAVISRQAKPIELGRGVLDIPGLMRAVKRIRYSGCCSIEHEMDMTDPYP
                     GIAESVGFFRGVLKAEG"
     gene            complement(8001..>8596)
                     /locus_tag="VGQ51_09785"
     CDS             complement(8001..>8596)
                     /locus_tag="VGQ51_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEV9036903.1"
                     /translation="LHVEGDRCAIIHCRLLGNQDTLYAGTENSRQYYADCFIEGTTDF
                     IFGEATAVFSRCEIRSLMNSYIAAAATTPRQKFGLVFLDCRLTADTAARRVFLGRPWR
                     PYARTVYIRTEMGAQILPAGWDNWRNPENEKTAFYAEYGSSGPGAAGKRVGGAKQVVA
                     SGKRVGWSHQLTGREARKYTPVNILGNWVEAYISGTK"
BASE COUNT         2026 a         2695 c         1914 g         1961 t
ORIGIN      
        1 gaaccagctg ttcgccggcc atctcttcga acccggccag ctgctcgacg ccaacaaata
       61 ttttattatc ctccccgacg gcatcggaca cgggaaatcc agcaaaccga gcgacggtat
      121 gcacatgcgc ttcccgaaat acacctatga cgatatgatc aaggccgatt acgacgtcct
      181 cacccaacaa atggggatca atcacctccg cctcatcatc ggcacttcga tgggtgctat
      241 gcactgctgg gtctgggcgg agacataccc cgacttcgtg gacgcctgta tggccaacgc
      301 cagcctgccc gtagagatcg ccggacgcaa ccgcgagtca cgctatgccg ctatcaaatg
      361 cgtcgagtcc gatcctgcct ggaagggcgg tgattatacc gaagaacccg ccaccggtct
      421 tcgcggcgcc tatacctcga tgatgtggat gaccagcagt ccttataata tgcagaaaag
      481 atccccgacg cgagagcagt cggaaagagg ttatgacttc gggtcggaga atttcatcaa
      541 acaccaggac gccaacgata tgatctatgc gttcgatgct tcgcgcttct ataacccgga
      601 acccaaactt agcacaatta aatgcacctt ctacgccgtc aattcagccg acgacgaatg
      661 caacccgccc gagctgggca ttctcgacag agatattaaa ctgatcccga aaggaagata
      721 tatcctgata ccctggtccg aaaaaacctc cggccacggt acacattcca atcccacggt
      781 atggggcgac tatctgaagg aattgatgga agccagcgaa cctggacact aacggaacaa
      841 caccacggca aacccgattt taccattggc gctgccattg atgtaaatat cataagggat
      901 cacgacctgg tccggggcca cctgtctggc ctcctggaag ttcagtgtat ttttcaaccc
      961 ccaaaaaaga ataccttcat tggtcacctg gttccccccc gggtcgatca cagtcgtcga
     1021 cgcatatacc ttctccccgt gtacaaaact gttataaagg aacaccatgc ggttatacat
     1081 cggcacgatc atataagata gattgggaga gttcaattcc gtcacctgct cctggctgat
     1141 cattccgctc cagcaactgt ccgtacgacc cgcggggaaa taataaaggc tcagatcacc
     1201 acggtcatga tacggcggat tcacggtcgc ccgataacgt gcatacccat acttcggctc
     1261 cggcaaatgc cgcatcagcg acgaaggacc catactgggg tcgggaattg cattggatgc
     1321 gaataacaac gcaggatccc agccctcatc atagtcctcc tcatccatct gttcatcggt
     1381 gaatggcctc ccgtattcct tcaacaacag aaaacccctg ccgggcaccg ctacaaaatt
     1441 ctccttcacg atattctcat tccggaccct tttcccggcc aacgtcgaca gacgatagga
     1501 ggagtcgaaa ctgaacatct tcgtgttcat ggaatagtgc acgacttcga catcctttag
     1561 cggcgcgtaa tgaaacgtcg aaaggatccc cgccacagcc tcaccattcg agttgtccag
     1621 cgacaggcac aggtcctcca tcgaggaggt ccccggcata ttgatctcct tagcgacgac
     1681 ggcggtatcc accgttgaaa aatggaatag aaggaaattg tgactgttcc gcgaaggcgc
     1741 gagcatcagc cagtccccgt tgttggcgag cttcacatca cccgtattga aatcttccag
     1801 cggaaaaccc gaataatcat cctggatcat cggctgcgaa agaaaaggat gcctgtacac
     1861 ccgttgggaa agcagctgcc agtcctgatc gaacaggaag gcatgcatcc ggggggcgcc
     1921 ggagggcaaa aattcgaatc ccagcatcag gatcttcttt ctgtcctgcg accgcacgag
     1981 catgaagctg ttgccgggtt cattgaaggg aaaaacagcc acgttgtgat cgggtcccgc
     2041 catggtcccg tccggattat aacgctgtag ccgcacctcc acctccttgc catcggcatt
     2101 caacaaatgc aatctgtcga aactgtcatc attgatcacc aggtactcct tgatactgct
     2161 gctcacatac gacggatcac cgaggacatt gatgacattc atacgcgaat cgtatatgac
     2221 gaaattccgc tcctggctgg ggtcgttgct atgcctgctg acctttctgc ggttgatgga
     2281 ggatacccag aaaaaatcgc ccacttttcc cagcatttcc tggcgtaaag tgaacctgtc
     2341 gctgtagggc tcacaatacg acacctgctg ggcataaagt aaggaacagt gagaaaaaat
     2401 gaggaacagc aatggtataa cttgcctcca tcccataact acctgcttcc ttttaaagtt
     2461 aagccttttt ttgcctcctt caccgcatac tctcaacggg cataaaatcc ttcctgcgct
     2521 gttgtcgcat tccgctatta agctatctct ccattccccg atcgggaaaa attttcctgc
     2581 tgtgggagaa aatgtccata cattatcctc catccatttc gcataacagc ggatcggaag
     2641 atataagaac ctgctaatca tagtgataaa aaagtttttt tgaccctggt tttcagcatc
     2701 ccttccggca cgtagcttgt cttaacatac tcggtttttc ataggatatt ggatttaaaa
     2761 acggggctgg atttctatcc tggccctttt ttattttatg ccgcatcctt cctttttcct
     2821 gacaccactg aaatagcctt ttcgccattt tccttttttt tcttggcagc cttaacatcc
     2881 cgcttctggc atttcccccc ttttttatgt agttttgaag tgctaatgtc ccttattcat
     2941 gcgtatcgtg ttgtccgtca tattcggcat tgtcctgacc ctacgactct ccgcccatcc
     3001 taacgtcagc accggcgcct tacccaaatg gctgtacgat ttccatccac atctcgataa
     3061 gacaccctcg gacgaagaca tcagcaatgg ctactactat aacctgctcg atctccaaag
     3121 caatctcgcc tgcaatacgg agtacacaca ttatgtccgg accatcgtaa accagtcggg
     3181 tgtccaaaat gcatccgaag tctcggttac cttttctccg caattccagc aggtgatctt
     3241 tcaccatata gatatcatcc gcgatggcgc tatccttcac aggcttcagc agggaaggat
     3301 caaagtagtc caggaagaga cggacgcgga cgagtttcag tataatggct tgaaaagggc
     3361 attccttacc ctcgaagacg tccgcaaagg ggaccggatc gatgttgcat tctctgttgt
     3421 aggtttcaac ccggtattcg acgacaaata ctcggatgat ttctcgttca acagcgagac
     3481 ggctgtctgt aattattata aaacgatcat taccacgccc gaccggcccc tgcatatccg
     3541 caccatcaat aacgcaccgc caccggtcga acaacgtata gataaccgcc tcgtctacac
     3601 ctggaataat cctcacatgg gctccgacga cgccagaccc gcatcgaatg ccccttcatg
     3661 gtttaacggc tatccgacgg tctatgtgac tgaatacccc gattggccgg ccgtcatcgg
     3721 ctggggcctt caaacattca accaatacca tttttccctt cccgccggtc tgcaacaaaa
     3781 gatctccgat tggaaaacgc gctcaggagg ggataaggtt gcttttacct cgctcgccct
     3841 gcgttttgtc caggatgagg tgcgatatct cggtctggag ataggcgcca acacccatcg
     3901 cccccaccag cccggcgaag tctttacccg ccgttttggc gactgcaagg acaaagcgct
     3961 gctgcttacg accatccttc aggaggaaaa aataccggcc ttcgtagcgc tgatcaatac
     4021 cgatacccgt agccggctga ctgccgtcgc accctccccc ggagccttcg accatgccat
     4081 cgttgccatc catcggcccg acggaaaata tgacttcgta gaccctaccc tgactaatca
     4141 gagaggtccc tttgccgaaa tcttcgttcc cgcatatggc tatgcccttg ttctacgcga
     4201 gggagaatct ggcctcgaac ccgtaacgcc cggaaagatc aacgactact cgatcatcga
     4261 gacactggac gcgccatggt acgacagcgc ccatttctcc atcacgtccg tctatgccgg
     4321 cggtgccgct gacgagattc gaggtatgtt tgcggaaaaa agcaaaaagg atctcgggga
     4381 gatttaccgc aagtattacg ccactctttt cgatgagatc cgccaggacc gcgacatcac
     4441 ttataaggat gacagtacca ggaacatggt gactgtcgga aaacattatt ccattccgca
     4501 ggtgtggaag aacggcagaa atggccagcg atattttgac ttcgtcacca agatcatcga
     4561 acaaaacctt cccgaccctt ccgacgcgcc cgacaatatt cccatctccc tgccctatcc
     4621 tttcaatgta cattataccc tcgaactcca tctgcctgaa ggctgggcat tcgaagagct
     4681 gcatgtgaaa aatgatgcct tccagttcga cttcgagcct gtccccaatg gcaataacgt
     4741 atcgctgcat tatagcctga aaactttcga tgaccatatt tccgcggcag ccgtccgcag
     4801 gtacaaggaa gactataaga acatcgccga taaaatatct ttccggttgt ccggaacacc
     4861 cggctcgggc gggtcgtttg gcgaaaacag cgcgacatcg gatacgccga ccccatctgc
     4921 tatggctctc agcgattgga aggcctgctg gcccgccata tggctcacgt tcttcttttc
     4981 tttgtttttc tcccggctct ttcaacgcct taactcgcgc agcgaagaaa ccctttatgc
     5041 tcccggctcc ggctacccgc tcggaggctg gctcatcctg ctcggcatca gcatcgcatc
     5101 gataatagta ttcgagctca tccgcctggt ccagtccgat tattacagtt atagcagctg
     5161 ggccacctat ggcaatgccg gcggagtcgc ccttcaatat ctctacctga cccaactggc
     5221 tatccagctt actctcatct gcggagccgc cgccctcctg ttctggttca tccggaaaag
     5281 agacatcttc ccccggatgt tcctctggta tgcaggtatt ttgctcaccg gccgcatcct
     5341 cctcctcgtt cttttctatg cgatgcccat tccggcatcg ctgatcggat accggacgat
     5401 acagatcatg gcgctcgtcc gatcgggcat ctatgccctc atctgggccg cgtatatcat
     5461 tcggtccggc caggtaaaaa gcaccttcct cgaacccttc cgcgaacgca tccggtaaaa
     5521 aatggcgcct gcaccttcgg tgcaggcgcc cccgactctc actcctggcg acccgtccaa
     5581 caacacctta ctgcttgatc agatacccga agatcccatc cgcctcatca gcaggtccgg
     5641 ccgtaaaata aagccggttc ggatcgatgc tcgtcgcagt ggccggcgca aaccccaacg
     5701 cccacaggcc gtcgatgact atggtcttat tgtgtgacct caattgcccc aaaaattctc
     5761 catcagggct gaatacattg atacgtccat ctccaaaatt gccgatcagt accaccggct
     5821 ccgacacacg cttcccgtcc tctccgaacg acccttttcc cttattactg tcgtccccgc
     5881 cgcctttctc accgctcata tcctccatgg acaaccagcc cgccggcgcc catgctgcac
     5941 cccagggcgc attcaggcta cccctcgagg caaaccgatg gacgaacgac ccgtcggtat
     6001 tgaatacatc cacaatgcct ttcccgggcc catgcatctc atctatgctt cccggtgtct
     6061 gctcggcata cataacgaaa agccattggc ccaccggcac aatattgaac ggcgcataat
     6121 gcgcgggcaa tgccggatca tggaatggca tagaaaccag gtggaaaccg gtatcccaaa
     6181 cgtcgatctt cccggtcata aagttggcgg cataaatgaa attctgcccg gggccggtgc
     6241 tgtccagcgc cagaccctta taaaccgatg tggccgaccg gtccctgata cgttgtgcat
     6301 tgttcccgtc cgcgccgttc caacccgaca atattccgtc ttccccgaca aaaaggaaat
     6361 tggcgccctg gtgattggcc aggacaaacc ccttcccgcc gctgaacacg atcccgctcg
     6421 gtgccccgcc tacactgtcg gtgggagatg gaatgttcac cggcggacga acgatcgccc
     6481 cttcgcccgt atacaattca ctgacatgat ctccgttcgc atttacccat gcgatgccgg
     6541 atggcgccca ggccagcccc cagccattct gcaaagtggt atccaccagt ttcggcatat
     6601 acatcgcctt attggccacc aggaccacct gctcgaaatt ccgcagatct ttttgagtga
     6661 tattggattt atggcaagca acggccatta ttccgccaaa taacagccac atcccgccac
     6721 gcgtcagcgg cgacaaccgc attccaccgc gcaccagacg cagcaacccc ttgccaccac
     6781 gcgtgagcca cagaaacccc ttgccaccgc gcgtcaccgg cagcggcata ctaaatcgtg
     6841 taatcttgtt cataatcagc aattttaagt tggaattgag tgatgcaata agcgtccatc
     6901 cagccatcgc aggatgcgat cgccgttcga caaaccaaaa acgaagggta acccctgtgc
     6961 ggagaggtaa gatccggccg gcctttgttt ctacagtgga ggggcgctgc gggtcacagc
     7021 gggtaagtta aacaaacgga agccctccac tgccagttgc ctggaccctg gagggctttt
     7081 tcaaaaaaat tttcggaggg cctatccctc cgccttcagg actcccctga agaaccccac
     7141 cgactccgca atccccggat atggatcggt catatccatc tcatgctcga tgctacaaca
     7201 accgctataa cggatacgct taaccgccct catcagtccc ggtatatcca gcacgccccg
     7261 ccccagctcg atcggcttcg cctgcctgct gatgacagcc aggtccttga tatgcatgtc
     7321 gaagatcctc ggcgcataat cgttgacgat cttcacgatg tcggcgccgg ctctggccgc
     7381 gtgaccgata tcaagacata atcccatccg gtcatccagt cccttgatat gttcgtatac
     7441 atccaccgga gaaggataca gcttatcctc gggtccatgg ttatggatgg caacccttat
     7501 ccggtatttc tttactttcc cttccacata ccccagcagt tcggggttag gcgctccgat
     7561 gatcaggtcg accccgagca tcttcgcata ttcgaaggcc ctgtcgacat cggcctcact
     7621 cttcatgtaa atgacgccgg ccccataaat gcgaatgccc gcggcattga aagccccaac
     7681 cacctgggcg atcttgtccg ccccgctgtc cagtggtaaa taaaaatctt tgatggatat
     7741 cgcatcgacg gccagccgtt tcatcatggc gatgccctga tccatcggga catgcaggaa
     7801 ggtatagccg gcaattccca gatgcaggtc cttcgggatt tcaccctttc ccccgccttc
     7861 cgttttcacg ttaccatcca cccaccccaa ccctgccaca ccagcccctg ctaccccaag
     7921 ccccgctacc cccgcccccg cgatcccaag ccctgtgcgt aagaaatttc ttcttgaact
     7981 cataatagtt gatttgatgt ctattttgta ccactaatat acgcctctac ccaattaccc
     8041 aggatattca ccggcgtata cttccgcgcc tcccgacccg tcagctggtg tgaccacccc
     8101 acccgcttcc ccgacgccac cacctgcttc gccccgccca cccgcttccc agccgccccc
     8161 ggcccgctgc ttccatactc ggcatagaac gccgttttct cattttccgg attccgccaa
     8221 ttatcccacc ccgccggcag gatctgcgcg cccatctccg tccggatata caccgtccgg
     8281 gcataaggtc tccagggcct gcccaggaat acccgccgcg ccgccgtatc cgcggtcaac
     8341 ctgcaatcca aaaaaaccaa cccgaatttc tgtctgggtg tcgtcgcggc cgccgcgata
     8401 taggaattca taagactccg tatctcacac ctgctgaaaa ccgctgtcgc ctctccaaaa
     8461 ataaaatccg tcgtcccctc gataaaacaa tccgcataat actgccggct gttctcggtg
     8521 cccgcataca acgtatcctg attccccagc agacgacaat gaatgatggc gcacctgtct
     8581 ccttctacat gcaacg
//
LOCUS       DASYSV010000328         4607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1087772, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000328 DASYSV010000000
VERSION     DASYSV010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087772"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VGQ51_09790"
     CDS             <1..454
                     /locus_tag="VGQ51_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV9036904.1"
                     /translation="LLRLTSEQWQEVINTNLTSVFNMTKQVIRPMMKAKQGSIINMSS
                     IVGVSGNAGQSSYAASKAGILGFTKSVAKELGSRNIRCNAIAPGFIETDMTHYLKEGD
                     GAKKFMENIPLGRFGRAEEIANTTLFLASDLSSYITGQVLSACGGLNI"
     gene            463..1098
                     /locus_tag="VGQ51_09795"
     CDS             463..1098
                     /locus_tag="VGQ51_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV9036905.1"
                     /translation="MTPIEKLLKDNRGWADELKSEDPEYFQRLAKLQTPDFLWIGCSD
                     SRVPADRITGTQPGEIFVHRNIANLVIHTDVNLLSVLDFAVNHLKVGHVIVCGHYGCG
                     GIKAAMTNHDYKYVLNMWLRNIKDVYRLYSKELDAISDEENRADRLTELNVKEQVMHL
                     AKTSIIQRAWKEERRPQLHGWVYGLKDGLIKPVFNMDSHSAIDPIYQYDDL"
     gene            complement(1134..1907)
                     /locus_tag="VGQ51_09800"
     CDS             complement(1134..1907)
                     /locus_tag="VGQ51_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV9036906.1"
                     /translation="MYQETREGTVKSEKLNGVSTIEFHHPQSNSLPGRLLEAIAQEIH
                     AAGDDSETRVIVLRSGGNKTFCAGASFDELLAIETPEQGLHFFSGFARLINAMRKCPK
                     FIILRVQGKCVGGGVGIAAAADYAIATEGADVKLSELAIGIGPFVVGPACERKLGVAH
                     FATLAINATGWRDAEWAKEKGLYSELHATSIEMDAAIERLAGNLANSNPEAMALMKQH
                     LWLGTEHWDQLLIERAGMSGRLVLSPFARQAISALRPKK"
     gene            complement(1920..3965)
                     /gene="paaZ"
                     /locus_tag="VGQ51_09805"
     CDS             complement(1920..3965)
                     /gene="paaZ"
                     /locus_tag="VGQ51_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEV9036907.1"
                     /translation="MTPKLGNYITGRWITGDGEGQPLYNAYNGEILTHASTKGLDFDS
                     ILRYARETGNPALRRMTFPERGRMLRSLALYLRQHLEEFYRISFLTGATRSDSWIDLE
                     GGIGNLFSYASLRRKFPDEPFCPDGEGQSLGKAGTFMGQHILVPKEGVAVHINAFNFP
                     VWGMLEKIAVNLLAGMPAVVKPATVTSFLTEAVVRRIGESGILPDGALQLICGSAGDL
                     LDYVGSQDVVTFTGSASTGQLLKSHPRIMAENVPFNMEADSLNCLVLGEDVAPGMPEW
                     DLFIKELRKEMTVKAGQKCTAVRRIFVPENKLEDARKALIHAISQTPIGNPENEKVRM
                     GPLAGQSQREEVRAQVSKLLASSQLIYGSLDSVELIEGDARKGAFLSPLLLLNEHPFT
                     SEEPHTIEAFGPVSTLMPYSDTDSVIQLSKLGKGSLCSSIVTANADIARRYVLGAATH
                     HGRILILNSECAKESTGHGSPLPSLVHGGPGRAGGGEEMGGIRGVKHYMQRVAVQGSP
                     TMLTAVTHNWQPHAMQSMDEKHPFRKYFEELRIGDTVVTHKRTVTEADISNFANLSWD
                     HFYAHTDHTSLAGTVFERPVAHGYFVLSAAAGLFVDAGKGPVLLNYGLEECRFLKPVY
                     AGTTIGVRLTCKEKIAQEKKEENETPRGIVKWYVDVYDETGESVAVATILTMVQRKD"
     gene            4056..>4607
                     /locus_tag="VGQ51_09810"
     CDS             4056..>4607
                     /locus_tag="VGQ51_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV9036908.1"
                     /translation="MEAIYSWINYAPPADAELLDSYSRTITGVVGQVAEAVVHIQVKK
                     PANDRRSREFKLMPASGSGFIISTDGFVVTNNHVIENAKDIRVSLADGRTVAAELKGA
                     DPSTDIAVLKIDVTGLRVLSFADSEALQPGQIAIAIGNPLGLQHTVTAGVVSALGRTL
                     RASNGRLIDDIIQTDASLNPGNSG"
BASE COUNT         1008 a         1226 c         1330 g         1043 t
ORIGIN      
        1 cctgctgcgg cttacctccg aacaatggca ggaggtgatc aacaccaacc tcaccagtgt
       61 tttcaatatg accaaacagg tgatccggcc gatgatgaag gccaaacagg gttccatcat
      121 caatatgagc tcgatcgtag gtgtcagcgg gaatgcgggg caaagcagct atgcggcctc
      181 caaggccggc attctcggtt tcacgaagtc ggtagccaag gagctgggta gccgaaatat
      241 ccggtgcaat gcgatcgctc cgggtttcat agagacggat atgacacatt atctcaagga
      301 aggcgacgga gcgaagaaat tcatggaaaa cattcctttg gggcgttttg ggcgcgccga
      361 agagatcgcc aataccacgc tgttcctcgc cagcgatctc agctcgtata ttaccggaca
      421 ggtgcttagc gcctgcggtg gtttgaacat ttaaatacag ccatgacacc tatagaaaaa
      481 ttattgaagg ataaccgcgg ttgggcggat gaattaaaat cggaagaccc tgagtatttt
      541 caacggctgg caaagctgca aacgccggat ttcctttgga tcggctgtag cgacagccgg
      601 gtacccgccg accggatcac cggtacccaa cccggtgaga tcttcgttca ccggaatatc
      661 gccaatcttg tcatccatac ggatgtcaat ctgctgagcg ttctcgattt cgccgtcaat
      721 catctgaagg tcggacatgt catcgtatgc gggcattatg gctgtggagg tatcaaggcg
      781 gccatgacca atcacgacta taagtatgtg ctcaacatgt ggctcaggaa tatcaaggac
      841 gtgtaccggc tgtacagcaa agagctggat gcgataagcg acgaggaaaa ccgggccgac
      901 cggctgacgg agctgaatgt gaaggagcag gtgatgcacc tggcgaagac ctccatcatc
      961 cagcgtgcct ggaaagaaga gcgccggcca cagcttcacg gctgggtcta cgggttgaag
     1021 gacggattga tcaaaccggt gttcaatatg gattcgcatt cggcgatcga tccaatctat
     1081 cagtacgatg atctttaatg ttccgcggcg ttcctaacgc cccgggcgcg ggcctatttt
     1141 ttcgggcgca gcgcgcttat tgcttgccgc gcaaacggac taaggacaag tcttccgctc
     1201 atgcccgccc gctcgatcaa tagctggtcc cagtgctcgg tgccgagcca gagatgttgt
     1261 ttcatcaacg ccatggcttc cgggttggaa ttggcgagat tgccggcaag ccgttctatg
     1321 gccgcatcca tttcgatgga tgtggcgtgt agttcggaat agagaccttt ttctttcgcc
     1381 cattctgcgt ctcgccaacc ggtggcattg attgcaaggg tcgcaaaatg cgcgactccg
     1441 agctttcgct cgcacgccgg cccgacgaca aagggtccga taccgatggc cagctcactc
     1501 agcttgacat cggctccttc cgtcgcaatg gcataatctg ccgctgccgc gatgcctacc
     1561 ccgccgccga cacattttcc ctgcacgcga aggataatga atttggggca tttacgcatc
     1621 gcattgatca gccgggcaaa gccgctgaaa aaatgcagtc cctgctcggg cgtttcaatg
     1681 gccagcaact cgtcgaaaga ggcgccggca caaaaagtct tgttaccgcc cgagcggagg
     1741 acgatcacac gggtttcgct gtcgtcgcct gcggcatgga tctcctgcgc gatcgcctcg
     1801 agcaaacgac ccggcagcga attactttgc gggtgatgaa attcgatggt cgatactccg
     1861 ttgagttttt cggatttcac agttccttcc cgggtctcct gatacataat ttatgcgatt
     1921 tagtcctttc gttgcaccat tgtcaatatc gtagccaccg ccacgctctc cccggtctcg
     1981 tcatagacat ccacatacca ttttacgatg ccccggggcg tttcattctc ttcctttttc
     2041 tcctgggcga tcttttcttt acaggtgaga cggacgccga tagtggtacc ggcgtatacg
     2101 ggtttcagaa agcggcattc ttcaaggcca taattgagca ggacgggacc cttgccagca
     2161 tcgacgaaga gtcctgccgc cgcgctgagg acaaaataac cgtgtgccac cggtctttcg
     2221 aaaacggtgc cggccagcga ggtatgatcc gtatgcgcat agaaatggtc ccagctgaga
     2281 ttggcgaaat tggagatatc ggcttccgta acggtacgct tgtgcgtcac gacggtatcg
     2341 ccgatccgca gctcctcgaa atatttgcga aaaggatgct tttcgtccat tgactgcatg
     2401 gcatgcggct gccagttatg cgtgactgcg gtgagcatcg ttggcgagcc ctgcaccgcc
     2461 acgcgttgca tataatgttt cactcccctg atgcctccca tttcttcacc gccgccggcg
     2521 cgaccggggc cgccgtggac caacgaaggt aggggacttc catgaccggt actttccttc
     2581 gcgcattcac tgttgagaat gaggatgcgg ccatggtgcg ttgccgcgcc aagaacataa
     2641 cgacgggcaa tatctgcatt ggcggtgacg atcgagctgc agagcgatcc tttcccaagc
     2701 ttcgagagtt ggattaccga gtccgtatcg ctatatggca taagggtact gacgggtccg
     2761 aaggcctcga tggtatgagg ctcttcgctt gtgaaaggat gttcgttgag caggagcaac
     2821 gggctcagga aagcgccttt ccgggcatcg ccctcgatga gctcgacgct atccagtgag
     2881 ccatagatca attgtgaaga ggccagtaat ttggaaacct gtgcacggac ttcttcccgc
     2941 tgcgattgtc cggcaagggg gcccatacgc actttttcgt tctcagggtt accgatggga
     3001 gtctgcgaga tcgcatggat cagtgctttt cgggcgtctt ccagtttatt ttcgggaacg
     3061 aagatgcggc ggaccgcggt acatttctga ccggccttca ccgtcatctc ttttcttagc
     3121 tcttttatga acaggtccca ttcgggcatg ccgggcgcaa cgtcttcgcc gagaaccagg
     3181 caattgagcg aatccgcttc catattgaag ggcacatttt ccgccattat ccgggggtgg
     3241 gacttcagga gttggccggt agaggccgag ccggtgaaag taaccacgtc ctgcgagccg
     3301 acatagtcca ggagatcgcc tgcgctgcca cagataagct gtagcgcccc atcgggcagg
     3361 attcccgact cgcctatccg gcggaccacg gcttcggtga gaaaggaggt gacggtagcg
     3421 ggtttgacca ccgcgggcat acctgccagc aggttgaccg cgatcttctc gagcatgccc
     3481 caaacgggga aattgaaagc gttgatatgg acggcgaccc cctctttcgg caccaggata
     3541 tgctgcccca tgaatgtgcc tgccttgcca agggattgtc cttccccgtc tggacagaag
     3601 ggctcgtcgg gaaattttcg acggagcgag gcataggaaa agaggttgcc gatacctcct
     3661 tccaggtcga tccagctgtc ggagcgggtg gcaccggtaa gaaagctgat ccgataaaat
     3721 tcttcgagat gctgacggag atagagtgcg agggagcgta acattcggcc gcgttcggga
     3781 aaggtcatgc ggcggagagc aggattgccg gtctcgcggg catatcgcag gatcgaatca
     3841 aaatcgaggc ctttggtgct cgcgtgggtc aggatttcgc cattgtaggc attgtataag
     3901 ggttgacctt ctccatcacc ggtgatccat cggccggtga tatagtttcc aagcttcggc
     3961 gtcattgtac aatcgttttc gctggcgaag ttaccctttt ttgtgccagt ctggcagaca
     4021 aaaattctgg tcggcaattt gccggcaaag aatacatgga agccatttat tcatggatca
     4081 attatgcgcc gccggctgac gcggagttgc tggacagcta ttcgcggacg atcaccggcg
     4141 tggtagggca ggtggcggag gcggtggtgc atatacaggt gaaaaagcct gcaaatgacc
     4201 ggcgaagccg ggaattcaag cttatgccgg catcgggttc gggatttatc atctcgaccg
     4261 acgggtttgt agtcactaac aatcacgtca ttgaaaatgc caaggatata agggtctcgc
     4321 tggcggatgg tcggacggtc gccgccgaac tgaagggagc ggatccgtct accgatattg
     4381 ccgtgctgaa gatcgacgtg acgggtctga gggtgctttc ttttgccgac tcggaagcgt
     4441 tgcaacccgg ccagatcgcg atcgccatcg gaaatccact gggtcttcag catacggtga
     4501 cggcgggtgt ggtcagcgcc ctggggcgga cgcttcgggc aagcaacggg aggctgatcg
     4561 atgatatcat tcagacagac gcctccctga atcccggcaa ttccggc
//
LOCUS       DASYSV010000329        13197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2367932, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000329 DASYSV010000000
VERSION     DASYSV010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367932"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1930
                     /locus_tag="VGQ51_09815"
     CDS             <1..1930
                     /locus_tag="VGQ51_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV9036909.1"
                     /translation="DEPNQKCTGDISLTVPAKYVTLSNGKLTAQVKHDDHTRTDTWKM
                     ELPISPYLFMIAVGNFSVIHDNWHGKPVDYYMEPQYAPDAKAIFGYTPEAMSFFSKIL
                     GVDYPWNKYAQIRVRGFNGGMENATATEFNEDGQSSPRELADLPYQSGNVHELFHQWF
                     GDYVTCESWSNLALNESFADLGEILWAEYKYGQDVADEHLYLGEQGYLGNPEQWKESL
                     IRFHYADKQEMFDGVTYQKGGRILNMLRHYLGNAAFDKGLHLYLTAHALGNADAQQLR
                     LAMEEASGLDLNWFFNQWFYGGGHPVLDIAYRWDEATKKQSVYLKQTQPGQTFTLPMA
                     VDIYAGGQKKRYQIWMHDRSDTLTFSGLQKPDLVNVDAEKVLLAKKSDHKALSEFAFQ
                     YAHAPLYVDRIEAINAAGANQTDSMAQHILLSALNDRYYGLRIKAMQTLDLSDKTISI
                     AAERILLRLAVKDTSNLARAEAIETVAKLNVPDVIDIFKQALANSTSYAVQGAALNAI
                     LQTDPKEAFTLAKQYEKDSKGKLREAIISIYCQSGGNAEWPFVDYCFADPNSPLQYRF
                     TGRFAALAGSVSNPVYARQGIDAIANLVIELKKFNSMATFVKVLEKVKADRLKLNDAS
                     SAEYVDKAISRMNAANLK"
     gene            complement(2008..2202)
                     /locus_tag="VGQ51_09820"
     CDS             complement(2008..2202)
                     /locus_tag="VGQ51_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036910.1"
                     /translation="MWLELYDDAKSKNEVAAAERLWNHAMELLPAYIPMFQNLVAVYE
                     KNKNASDAMLRKLKEQIKTF"
     gene            complement(2268..2615)
                     /locus_tag="VGQ51_09825"
     CDS             complement(2268..2615)
                     /locus_tag="VGQ51_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036911.1"
                     /translation="MTCADGKRVHGAKRPDYFKPSPFPVKRDTITKLWKKLVMDDPPG
                     GLGIIKHLYAAKHTGTDDKTDSGLELRDIQVMYGHSSEAITARYKKRNREIEAKKEIL
                     AKSPAFSKRPNNG"
     gene            complement(2848..3327)
                     /locus_tag="VGQ51_09830"
     CDS             complement(2848..3327)
                     /locus_tag="VGQ51_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036912.1"
                     /translation="MVPDTNGRAYTMPEISTANPKDWYIWFRYTHNDKEVPRKFQGIK
                     PTAFRKDIFQVDGLNFALEKKKLASQLKPGYKSMLDFIKQVAVKEGYNLLPLTEFDRG
                     VCLNLIDECAKVRQFTNHNYNKHVAVLRSMFGELVQYRMLKSNPLTGFREKEEAEQV"
     gene            complement(3400..3487)
                     /locus_tag="VGQ51_09835"
     tRNA            complement(3400..3487)
                     /locus_tag="VGQ51_09835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3451..3453),aa:Ser,seq:tga)
     gene            3638..5512
                     /locus_tag="VGQ51_09840"
     CDS             3638..5512
                     /locus_tag="VGQ51_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9036913.1"
                     /translation="MKHLKAVNKYFWKYRWRFLLGLVFIILSNYFRILTPQVTGYVVN
                     SVEAAMKQHTGGASQGAATRQHTAAAPSQDTVNYDILVRKFIGKMDARHATFNEKVVI
                     CGITLLVLALISGGFLFLMRQTIIVMSRHIEYDQKKEIYDHYQQLDTNFYKTHSTGDL
                     MSRMAEDVSRVRMFTGPAIMYLANLLVTIGFSLFFMVRKDALLSLYVLTPLPILAITI
                     YYVNTIIHKRSEHIQALLSNLTTNAQESYSGIRVIKSFVQEAGMLRFFSHNSEEYRKN
                     AVNLAKVESIYFPSMGLMIGLSTLLTIMIGGLYVIYGKHGTDLGTITEFVIYITMLTF
                     PVSAIGWVASMVQRASASQKRLNEFLDTQPTIYSPSGAPSQRLQGNICFQDVDFTYPN
                     TGIHALSDFSLEIRKGEKVVIVGRTGSGKTTVAQLLLRMYDPDKGRILIDGTDIRHLD
                     LQDIRRQISYVPQDVFLFSDTVTGNIQFGVDRADEEKVHLAARYASVDKEIEGFSNQY
                     DTLIGERGVTLSGGQKQRISIARGLIKDPEIIVFDDCLSAVDAKTEKEILGNLYQYLR
                     QKTAIIITHRIFSLFDFDRIVVIEEGRIVETGTHQELMALNGYYTYLYEQQQAENNPA
                     "
     gene            5686..6126
                     /locus_tag="VGQ51_09845"
     CDS             5686..6126
                     /locus_tag="VGQ51_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HEV9036914.1"
                     /translation="MAYENNDKRMESVYSKRIRAGKRRTYFFDVRATRSNDYYLTITE
                     SRKKFNEDGYDRHKIFLYKEDFNKFLKALTEAIDYVKTELMPDFDFDAYNHDNVEGNE
                     GSYQETAVSDEVVEEEVRHTPPAASHTPTHTPTSAPTGEEVDKW"
     gene            complement(6176..7495)
                     /locus_tag="VGQ51_09850"
     CDS             complement(6176..7495)
                     /locus_tag="VGQ51_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9036915.1"
                     /translation="MKPIKILLLNYYWPPSGGPAVQRWLDITRYLSACGIQTDVVTVD
                     EKLATYPFVDATLITRIDPSTRTFRTGTSELFGIYKRFIGKGKVPASGLVDEPHPTLL
                     QKLARFARGNFFLPDPRKGWNNHAYDRAAALISSEKIDVVFTAGPPQSTHLIGLRLKK
                     HFPHIRWVADIHDYWTEISYLSKFYRTAIAGWFDRRLEKKVLQSADRIMTHCESSKTL
                     QCEKLAGRDCPKIFVHTMGYNELLFRPRPPEKQTFFEIIYTGIMADHYEPDMLFRALR
                     TAIHQRPDIPVKLKFVGSLASTIRDSISELGLGPLLEELPYVPHSAVVEELHNSTALF
                     LINPKFENEKIHVPGKIYEYLAVFKPILSISPHGSENEQIIARMHAGRNFSRSEETQL
                     SQYLVELMDRWQQEKNIDLPENPDIRVYGRRHEAERLGHIIEDLSPR"
     gene            complement(7505..10060)
                     /locus_tag="VGQ51_09855"
     CDS             complement(7505..10060)
                     /locus_tag="VGQ51_09855"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV9036916.1"
                     /translation="MNSQLLKKALPHILAIAVFLIVAVVYCRPALQGKGVGQSDVDQW
                     KAMAQQSFDYKEKHGQFPLWTESAFGGMPGYTIAMPGNNGINFHYLTQALTLGLPQPI
                     SFFFVACLCFYILMCVLQIDPWIGVMASLAYAYCSYDPQIIVTGHVTKMDALTVAPGV
                     IASLLLLFRRRYLWGTALLAFFFASELAAGHLQMLYYTIICMGLLTLFYGIQSIRKGH
                     GKDLAIAVGLAIIACGIGYGTNMSGNLPLQEYAKETMRGGRTELTKAANQGSSPGLER
                     GYAFQWSYGVGETMTLAVPDIFGGGNGDRTAVGEDSKAVDVMTQNMGLPGDFSASLVS
                     ESTYWGDQPFTSGPVYIGAVICFLCIFSLVVIRGWAKWWLLSTIVVGIILAWGKNFPA
                     INNFLFDHLPLYNKFRAPSQALVLPQLAAPILAALGLQHLLVSGEDPAKLWKKFRLSA
                     YIAGAGLVLMIGFYFSTHYQGVNDAQVRERFAQYGMRALSRGQQPTPEIQQQAMSAAG
                     SVMRGLEEDRKSMAGSDLLRSIILIALAASLLGFFIRGKFRPVILLAGILVLSTYDLL
                     GVSNRYLTADSFQDPSDLQSMLTPTPADQQINADPEKNFRVLDESAGGDVFQSARASA
                     FHNSIGGYSPAKLGLYQDLIDSQLAKGNMQVYDMLNTKYIIQQDPRNGQPVARINPAA
                     FGPVWLVKAIHYVRDGKEEMKALDSINVRDTVIIQQQFASQIPFAPMPDSSASIKLVQ
                     NDLDKIDYNFSAKTNQFAVFSEIYYSKGWDAFIDGKKTGYLRVDYALRGMPVPAGQHT
                     IEFRFEPRSYHIGSMMTGWFSLAIYLLLIAGVVMEYRKRNPRKSGAPGDKAKV"
     gene            10137..10442
                     /locus_tag="VGQ51_09860"
     CDS             10137..10442
                     /locus_tag="VGQ51_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036917.1"
                     /translation="MRPLRFFSRVAFICNICFLVVLVASNRRWLNSLPDNEIISDMIV
                     LGYLVSAVLNLLVNSALILLLLIGRLRRAGVPVWLLIVNFVFFIVQILLLIANRHLK"
     gene            10439..11239
                     /locus_tag="VGQ51_09865"
     CDS             10439..11239
                     /locus_tag="VGQ51_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEV9036918.1"
                     /translation="MIQNILKDRPTKLLLGFTAFFCCNALIADAIGTKLFSLEKIFGL
                     HPANWTLFGQSGLGFTLTCGVLLWPLEFVITDIINEYYGPRVVRRISYTAVVLISYAF
                     LMYFMAIHVPPADSWIASSAKQGVPNLQDSFSAIFGSTSLIIVGSIVAFLVSQVVDVT
                     VFHQIKKRTGHKHLWLRATGSTLVSQLVDSYIVLFIAFSQYFTWQQILAIGMMNYIYK
                     ASMAIVLTPLIYLIEGRIERYVGHATAHKMKLAAMGQEETEGPIPTAG"
     gene            complement(11295..11840)
                     /locus_tag="VGQ51_09870"
     CDS             complement(11295..11840)
                     /locus_tag="VGQ51_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9036919.1"
                     /translation="MNKLSTDKTVNTMDERISIRPADLDDINTIGFLAQQIWPDTYGK
                     ILSAEQLKYMLNMFYSPASLRRQMVDEHHSFVLMEQGEEAIGFASWSASAEPGVYQLH
                     KVYFLPGQQGKGLGRSVLQFIFGEIGPEGAKTLRLNVNRYNKARQFYERMGFAVKGEA
                     DVPIGHGYFMNDYVMEIAVPG"
     gene            complement(11797..12543)
                     /locus_tag="VGQ51_09875"
     CDS             complement(11797..12543)
                     /locus_tag="VGQ51_09875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEV9036920.1"
                     /translation="MYLLALALAVAPGIAICLFIWSLHNMGKASMRIMIVSFLLGALA
                     TLPPLAIQWLSVDVRNEPWRHSILSYIWYAFAVVAFSEEGSKFLVLRFYAYPKEMFRE
                     PFDGVVYCVAIGMGFATVENIEYVRQFGLETGVSRFFLAIPAHAAFAVLMGYSVGRAR
                     VERHRSFLLMTKGLAIAVLFHGSFDFFLFLQQNHEVTRYLSNGVLPFGAFATFYIAVR
                     LALRALRMHKIAKEEEDEQTFNRQNREHDG"
     gene            complement(12558..>13197)
                     /locus_tag="VGQ51_09880"
     CDS             complement(12558..>13197)
                     /locus_tag="VGQ51_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036921.1"
                     /translation="ELDSRLLSPLFLSWLWGSTCWIPGALRLKPGTQSESATPGTHQL
                     KPGTPTPSSPRWKKAAILLCLAAAICFFTGEMQTFRENWDGIHDAGIPGYTENDWRNS
                     ETMAFVRDHQDSLRNIPRLGTLYSDAFEGLWFLTGVRSDLLPHKDNAEDIRYMLKEDR
                     FTVIWFDDAVNTDLIDVDFLKSRKQLIREIHFKDGAIYFFQTAPPPAQASPN"
BASE COUNT         3178 a         3373 c         3476 g         3170 t
ORIGIN      
        1 cgatgaaccc aatcaaaagt gcaccggcga tatcagcctg acggtgccag ctaaatatgt
       61 tacgctatcc aacggaaaac ttacagcaca agttaaacat gacgaccata cccgtaccga
      121 tacctggaaa atggagcttc cgatttcgcc ttacctgttt atgatcgccg tgggcaactt
      181 cagtgtcatt cacgacaatt ggcatggaaa gcccgtggat tattatatgg agccgcaata
      241 tgccccggac gctaaagcta tatttggtta cacgcccgaa gcgatgtctt tcttttcaaa
      301 gatattgggc gtcgattatc cctggaacaa atacgcacag atcagggtgc gcggtttcaa
      361 tggcggcatg gaaaatgcaa ccgctaccga atttaatgaa gacggccaga gcagcccgcg
      421 cgaactggca gacctgccgt accaatcagg caacgtacat gaattgttcc accagtggtt
      481 cggggattat gtaacctgcg aaagctggag caacttagct ttgaacgaat cctttgccga
      541 cttgggtgag atcctttggg ctgaatataa gtatgggcag gacgtagccg atgaacacct
      601 atacctgggc gagcagggtt acctgggtaa ccctgagcaa tggaaggaaa gcctcatccg
      661 ttttcactat gcggacaaac aggaaatgtt tgacggcgta acctatcaga aaggcgggcg
      721 gatcctcaat atgctgcgcc actacctggg caatgccgct tttgataagg gcttgcatct
      781 gtatcttact gctcatgctt tgggaaatgc tgacgcgcag caactgcgcc tggcgatgga
      841 agaagccagc ggcctggacc tgaactggtt ttttaatcag tggttctatg ggggcggtca
      901 tccggtattg gacattgctt atcgttggga tgaagcgaca aaaaagcaaa gcgtttacct
      961 gaagcaaaca cagcccggac aaacctttac cttgcccatg gcggtagata tttatgccgg
     1021 tggccaaaaa aagcgttacc agatatggat gcatgaccgg tctgatacgc tgacattttc
     1081 cggtctccaa aaaccagacc tggttaatgt agacgcggag aaagtattgc tggcaaaaaa
     1141 gagcgatcat aaagccttaa gtgaattcgc attccaatac gcccatgctc ctttatacgt
     1201 tgacaggatc gaagccatca atgctgccgg agcgaaccaa acggatagca tggcccaaca
     1261 tatcttactc agtgccctca atgatcgtta ttatgggtta cggatcaagg ccatgcagac
     1321 gctggacctg tccgataaaa caataagcat cgccgccgag cggatccttc tccggttagc
     1381 ggtaaaagat acgagtaacc tggcgcgcgc tgaagccata gagaccgtcg ccaaattgaa
     1441 tgtacctgac gtaattgaca tatttaaaca agcgctggcg aacagcactt cctatgccgt
     1501 gcagggcgcg gcgctcaatg cgatcctcca aactgatccc aaagaggcat tcacacttgc
     1561 caaacaatat gaaaaagaca gcaagggaaa actcagggaa gctattatca gcatttattg
     1621 ccaaagtgga ggaaacgccg aatggccttt tgtcgattat tgctttgccg atcccaattc
     1681 accattacaa tatcggttca cgggaaggtt cgcggccctg gccgggtctg tatctaaccc
     1741 ggtatatgcc cggcaaggta ttgacgcgat agcgaacctc gttatagaac tcaaaaaatt
     1801 taacagcatg gcgacattcg ttaaggtact ggaaaaagtt aaagccgata gacttaaact
     1861 aaacgatgcc tcttcagcgg aatatgtcga taaagcaatc agcaggatga atgctgcgaa
     1921 tttgaaataa ccacaatgac ccttcaatac taactttgag cggcataaaa aaagtatata
     1981 agaagcccag ctactgccct gtcttcgtca aaatgttttg atctgctctt taagtttccg
     2041 tagcatggca tctgaagcat ttttattctt ttcatacaca gcaaccaaat tttgaaacat
     2101 tggaatatat gctggcagca gttccattgc atgattccac agccgctccg ctgctgcaac
     2161 ttcattttta gatttggcat cgtcataaag ttccaaccac aattttacat tttggtcaag
     2221 cgaggcgata tctccattgc cttgagaatt acgaataatc gagcttctta accattgttt
     2281 ggccgtttgg aaaatgcggg agatttagcc aatatctcct tctttgcctc gatttcccga
     2341 ttcctctttt tgtatctggc agtaatggcc tccgacgaat gcccatacat cacctggatg
     2401 tcccttaact ctaaacctga atccgtttta tcgtcggttc ccgtgtgttt ggcagcataa
     2461 aggtgtttta tgatgcccag ccctccgggt ggatcatcca taacgagttt tttccacagc
     2521 ttggtaatcg tatctctttt tactggaaag ggactgggtt tgaaataatc gggcctctta
     2581 gctccgtgga cccttttccc atccgcgcag gtcatactac cgcaattccg tcgtaaaggt
     2641 gctccaaacg cataaaaatg gcctggaaag ctctttaaat tcatgggcaa taaaagttct
     2701 tgtaaatggg ggttaatggg caccctgcgt acgttttcct ttggccgaat tccagtatta
     2761 tagacgatgg acattattat gaaaagctgc gggtgaacct ttgacagatg ggagtggatg
     2821 aggtcttttt cttcgtctgt gtaatcttca tacttgttct gcctcctcct tttcccgaaa
     2881 gcccgttagg gggttgctct tcagcattcg gtattgcaca agctccccaa acatactacg
     2941 gagaacggcg acatgtttat tatagttgtg gttggtgaat tgcctcacct tcgcacattc
     3001 gtcaatcagg ttcaggcata cccctctatc aaattctgtc agggggagaa ggttgtagcc
     3061 ctctttgacg gcaacctgtt tgatgaaatc gagcatggat ttatagcccg gcttcaattg
     3121 actcgccagc ttcttttttt cgagggcgaa attcaggcca tctacctgaa aaatatcctt
     3181 cctgaatgcg gttggcttga ttccttggaa tttgcgtgga acttccttgt cgttgtgagt
     3241 gtagcgaaac cagatgtacc aatctttggg gttcgctgtt gaaatctcgg gcatcgtata
     3301 tgccctaccg ttggtatcgg ggaccatgct ttaagattgt accggaattt gtaccggcaa
     3361 ttgagagtta acaacctaaa gcattgacaa tcaaatagtt ggcggagaga gagggattcg
     3421 aacccccgga ggtgttaccc tcaacggttt tcaagaccgc cgcaatcgac cgctctgcca
     3481 tctctccggg tgcaaaataa aggtctgctg ggggaatgga caaatattaa ccaccttacg
     3541 ggcaaatatt tttgacggcg ggaagccagg tacgggcggc ggcgggcaag cgcctttttt
     3601 tggcggctgg ttcgtaaatt tgcataaatc cgaaggattg aagcatctaa aggcagtcaa
     3661 taaatatttc tggaaatacc ggtggcggtt tttgctgggc ctcgtattta tcatcctttc
     3721 caactatttc cgcatcctga cgccgcaggt gacgggatat gtcgtcaatt cggtggaggc
     3781 tgcgatgaag cagcataccg ggggggcatc gcagggggcg gcaacgcgac agcatacggc
     3841 tgcggcgccg tcacaggata cggtcaatta cgatatcctg gtccggaaat tcatcggaaa
     3901 aatggacgcg cggcacgcga cgttcaacga gaaggtcgtc atttgcggca ttacactcct
     3961 tgtgctggcg ctgatcagcg ggggctttct tttcctcatg cgacagacca tcattgtgat
     4021 gagccgtcat atcgagtacg atcagaaaaa agagatatac gatcattacc agcagctgga
     4081 taccaatttt tacaagaccc atagcaccgg tgacctgatg agccggatgg cggaagacgt
     4141 cagccgcgtc cgtatgttta ccggtccggc gatcatgtac ctcgccaacc tgctcgtgac
     4201 catcgggttc agcctgtttt tcatggtccg gaaggacgcg ctgctgagcc tctatgtgct
     4261 gacaccattg ccgatccttg ccattaccat ttattacgtt aatacgatca ttcacaaacg
     4321 cagcgaacac attcaggctt tgctgagtaa cctcaccacc aatgcccagg aatcgtatag
     4381 cggcatccgg gtgatcaaat ccttcgtgca ggaggcgggt atgttgcgtt tttttagtca
     4441 caacagtgaa gaataccgca agaatgcggt caacctggcg aaagtggaat ccatctattt
     4501 tccatccatg ggtttgatga tcggtctcag caccctgctg accatcatga tcggcggcct
     4561 ctatgtcata tatggcaagc atggtaccga tctgggtacg atcaccgagt ttgtcatcta
     4621 tattaccatg ctgacctttc ccgtcagcgc catcggctgg gtggccagca tggtccagcg
     4681 cgcatccgcc tcacagaaac ggctcaatga attcctggat acccaaccca ccatttatag
     4741 cccctccggc gcgccatctc agcggctaca gggaaacatc tgttttcaag acgtggattt
     4801 tacctacccc aatacgggta tccatgcact gagcgatttc agcctcgaga tccggaaagg
     4861 ggagaaggtc gttatcgtcg gcaggacggg tagcggcaag accacggtcg cccagttgct
     4921 gctcaggatg tatgatcccg acaaaggcag gatcctgatc gacggtacgg acatccgcca
     4981 tctcgatctg caggacatcc ggcggcagat cagctatgtg ccccaggacg ttttcctgtt
     5041 cagcgatacc gtgacgggaa acattcaatt cggcgtcgac cgggcggacg aggagaaagt
     5101 gcatctggcg gcccgctatg ccagcgtcga taaggaaata gaggggttct cgaaccagta
     5161 tgacaccctg atcggtgaac ggggggtgac gctcagcggc ggtcaaaaac aacggatatc
     5221 catcgcccgg gggctcatca aggatcccga gatcatcgtc ttcgacgact gtctgagcgc
     5281 cgtcgatgca aagacggaaa aagagatatt gggcaatttg taccagtatt tacgccagaa
     5341 gaccgccatt atcattacgc accgtatttt ttcgttattc gatttcgacc gtatcgtggt
     5401 catcgaggag ggccggatcg tggaaacggg aacgcaccag gaattgatgg cgctcaatgg
     5461 ctattatacc tatctctatg agcagcaaca ggctgagaac aatccggcat agcaaaaaag
     5521 gcgcctcctg aaggctggaa aatgctcccc ggaacacccg ttccgccaat cggataaatt
     5581 cgcctcaaat agtagctaca attgttgtcg aaattttgct aattcgaaaa caatactata
     5641 tttgtgactg cataattagt aattctcaac agaaaaaaat tatttgtggc gtacgaaaac
     5701 aacgacaaac gaatggaaag cgtctacagc aaaaggattc gcgctggcaa acgcaggaca
     5761 tatttcttcg acgttagggc aacccgtagt aacgactact atctcacgat caccgaaagc
     5821 cgtaagaaat tcaatgagga tgggtatgac aggcacaaga tcttcctgta caaggaggac
     5881 ttcaacaagt ttctgaaggc attgaccgaa gcgatcgatt acgtcaagac cgaattgatg
     5941 ccggatttcg atttcgacgc ctataaccac gataatgtag agggaaatga aggcagctac
     6001 caggaaacgg ccgtcagcga tgaggtcgtt gaagaggaag tccgtcatac gccgcccgcg
     6061 gcttcccata cgcctaccca tacgcccacc agcgctccca ccggggaaga ggtcgataaa
     6121 tggtaatata gtttatgccc tatagcggac ccctactttg taggggtctt tttatttagc
     6181 gcgggctcaa atcttcaatg atgtggccga gtctttcggc ttcatgccgt cttccataaa
     6241 cccggatatc cggattttcc ggaaggtcga tattcttctc ctgctgccag cgatccatca
     6301 gttccacaag gtattgactc agctgcgtct cctccgaccg gctgaagttt cgcccggcgt
     6361 gcatcctggc gatgatctgc tcgttctcgc tgccatgcgg cgagatgctc aatattggct
     6421 tgaagaccgc gaggtattcg tagatcttgc cgggaacgtg tattttttcg ttctcgaatt
     6481 ttggattgat caggaacagg gcggtactgt tatggagttc ttctactacc gcggaatgtg
     6541 gcacataggg caactcttcc agcagggggc ccagtccgag ctctgaaatg gagtcccgga
     6601 tggtagaggc gagcgaaccc acgaacttta gtttcacggg aatatccggg cgttgatgga
     6661 tagccgttcg gagggcccgg aaaagcatgt ccggctcata atggtcggcc atgatcccgg
     6721 tatagatgat ctcaaaaaaa gtctgtttct ccggcggacg gggccggaac aggagttcat
     6781 tatagcccat ggtatggacg aaaatcttcg ggcagtcgcg gcccgctaat ttttcgcatt
     6841 gcagcgtttt cgaggattcg caatgtgtca tgatcctatc ggcggactgc aatacttttt
     6901 tttccagccg ccggtcgaac cagcctgcga tagccgtccg gtagaatttt gacaaataag
     6961 atatctctgt ccagtaatca tgaatatcgg cgacccaccg gatatgcggg aaatgctttt
     7021 tcagtcgcag cccgatgagg tgcgtcgatt gcggcggccc tgcggtgaaa acgacatcga
     7081 tcttttcaga gctgatcaag gctgccgcac ggtcataggc gtggttgttc cagcctttcc
     7141 tcgggtcggg gagaaagaaa ttgcccctgg cgaaccttgc caatttttgc aggagggtgg
     7201 ggtgcggttc atccacgagg ccgctggcag gcactttgcc ttttcctatg aatcgtttgt
     7261 aaatgccgaa gagttcggag gtgcccgttc tgaacgtccg cgtggagggg tcgatccggg
     7321 tgatcagcgt cgcatcgacg aagggatagg tagccagctt ttcgtccacc gtcactacat
     7381 ccgtttgaat accgcaggcc gagagataac gggtaatatc cagccagcgt tgtaccgcag
     7441 gcccgccact gggaggccag tagtagttga gcaataatat tttgatcggc ttcatggcgg
     7501 tggattaaac cttggcctta tcccccggag cgccactctt ccggggattc cttttgcggt
     7561 attccatcac cacccctgca atcagcaaca ggtagatcgc caggctgaac caaccagtca
     7621 tcatcgatcc gatgtgatat gaacgcggtt cgaaacggaa ttcgatggta tgttgtccgg
     7681 ccggcaccgg catgccgcgc agcgcatagt ctacccgaag atagccggtc tttttgccat
     7741 cgataaaggc gtcccagccc ttgctatagt agatctcgct aaagaccgca aactggttgg
     7801 tcttcgccga aaaattataa tcgatcttgt cgagatcgtt ctggacgagc ttgatggacg
     7861 cgctgctgtc cggcatcggg gcgaatggta tctgcgaagc gaattgctgc tggatgatga
     7921 cggtatcgcg tacgttgatg ctgtcgaggg ctttcatttc ctctttgccg tccctgacat
     7981 agtggatggc tttgaccagc cagaccggac cgaaggccgc cgggttgata cgggcaacgg
     8041 gctgaccgtt cctggggtcc tgctggatga tatatttcgt attcagcatg tcgtacacct
     8101 gcatattgcc cttggccagc tggctgtcga tcaggtcctg gtagagaccc agtttggcag
     8161 gtgaatagcc gccgatcgaa ttgtggaagg cggaagcccg ggcgctttgg aagacgtcgc
     8221 cacccgcact ctcgtccaac acgcggaaat tcttttccgg atcggcattg atctgctggt
     8281 cggccggtgt cggggtcagc atggactgca gatccgaagg gtcctggaaa ctgtccgcag
     8341 tcagatagcg gttgctcacg ccgaggagat cataggtgct aagtaccaaa attccggcca
     8401 gcaggatcac cggcctgaac tttccgcgaa taaagaaacc gagcagcgac gccgcgagag
     8461 cgatcaggat gatcgatcgc agcagatcgg agccagccat cgacttacgg tcctcttcca
     8521 gcccacgcat cacactcccc gccgccgaca tggcctgctg ctggatctcg ggcgtaggct
     8581 gttgtccgcg cgaaagcgcc cgcatcccat actgtgcgaa cctttctctt acctgtgcat
     8641 cattcacgcc ctggtagtgg gtggagaagt agaaaccgat catcaggacg agaccggcgc
     8701 cggcgatata ggccgacagc ctgaatttct tccacaattt tgccgggtcc tcgccgctga
     8761 caagaagatg ctgaagaccc agcgccgcca ggatcggcgc cgccagttgc ggaaggacca
     8821 aggcctgtga aggagcgcgg aatttattat ataaaggaag atgatcgaac aggaaattgt
     8881 tgatggcggg aaagttcttt ccccacgcca ggataatacc cacgacgatg gtggatagca
     8941 gccaccattt ggcccatccc ctgataacga cgagcgagaa gatacacagg aaacaaatga
     9001 cggcgccaat gtataccgga cccgacgtaa acggttggtc tccccagtag gtggactcgc
     9061 tgacgagcga agcactaaaa tcgcccggca ggcccatatt ctgcgtcatg acatcgactg
     9121 ctttggaatc ttctccgacc gccgtcctgt cgccatttcc tccgccgaat atatcgggta
     9181 cggcgagtgt catcgtctcg ccgacgccgt agctccattg gaaagcatag ccgcgttcga
     9241 ggcccgggct gcttccctga ttggcggctt ttgtaagctc ggtacgtccg ccgcgcatcg
     9301 tctctttggc atattcctgc agcgggagat tgccggacat gttcgttcca taccctatgc
     9361 cgcaggcgat gatggcgaga ccgaccgcga tagccagatc tttgccatgt cctttgcgga
     9421 tgctttggat gccatagaac aaagtcagga gacccatgca gattatggta taatacagca
     9481 tctgcaggtg tcccgctgcc agctcggacg caaaaaagaa ggccagcagt gcggttcccc
     9541 ataggtatct gcgacggaat agcaggagca gactcgcgat cacgcccggg gcaaccgtca
     9601 gggcatccat cttcgtgaca tgacccgtga cgatgatctg cgggtcatag gagcaatagg
     9661 cgtaggccag tgaagccatt acgccgatcc agggatcgat ctgcaggacg cacatcagga
     9721 tatagaaaca cagacaggct acaaaaaaga agctgatagg ctggggaaga cccagggtga
     9781 gcgcctgcgt gaggtaatga aaattgatgc cattgttacc gggcatcgcg atagtatagc
     9841 cgggcatccc gccgaatgca ctttccgtcc acagggggaa ttgtccatgt ttttccttgt
     9901 aatcgaagga ctgctgcgcc atcgctttcc attggtccac atcgctctgt ccgactccct
     9961 tgccctgcaa tgcaggacgg cagtatacga cggcgacaat taggaaaacg gctatagcca
    10021 ggatatgggg taatgctttt ttcagtagct gggaattcat atgaacctca ttattcccgg
    10081 caaaataatg gtatttaaat ggtattttgc ccggaaaacc tgatttttgg tcaaatatgc
    10141 ggccgttacg ttttttctca cgggtagcct ttatctgcaa tatctgcttt cttgtagtcc
    10201 tcgtggcgtc gaacaggcgt tggctaaatt cgctgcccga caatgagatc atatcggata
    10261 tgatcgtctt aggttatctg gtatccgcag tcctcaatct tctcgtgaac tcggcgctga
    10321 tcctgttgtt gctgatcggt cgtttgcgcc gtgccggtgt tccggtctgg ttgctgatcg
    10381 ttaattttgt ttttttcatt gtccaaatac tactgttgat cgccaatcgt catttaaaat
    10441 gatccaaaac atactaaaag accgaccgac aaaattatta ttgggtttta ccgctttctt
    10501 ttgctgtaat gcgctgatcg ccgacgccat cggcaccaag ctgttctcgc tggagaagat
    10561 attcggcctg catccggcga actggacctt gttcggccag agcggcctgg ggtttacgct
    10621 gacctgcggc gtgcttttat ggccgctgga gtttgtgatc accgatatca tcaatgaata
    10681 ctatgggccg cgggtcgttc gccgaatttc ctataccgcg gtcgtgctga tctcctatgc
    10741 ctttttgatg tactttatgg ccatccatgt gccgccggcg gattcgtgga tcgccagcag
    10801 cgctaaacaa ggggtgccta accttcagga ttctttcagc gccatcttcg gctcgacatc
    10861 actgatcatc gtgggcagta tcgtggcttt cctggtcagc caggtagtcg acgttacggt
    10921 gtttcaccag attaagaaac ggaccggtca caagcatctt tggctccgcg caacgggatc
    10981 gaccctggta tcgcaattgg tggatagtta tatcgtgctc tttatcgcct tcagccagta
    11041 cttcacctgg cagcagatat tggcgatagg gatgatgaat tatatctata aagcctcaat
    11101 ggccatcgtc ctgacgccgc tgatctatct gatcgaaggc cggatcgaaa gatatgttgg
    11161 gcatgcgacc gctcataaga tgaagctggc ggccatgggc caggaagaga ccgaaggtcc
    11221 gatccccacg gcgggttgat ccggtggagg catccttgtg cgttggcggc atccggcgct
    11281 gcaatgcggc ggcttcagcc gggtacggca atctccatta cataatcgtt catgaaatag
    11341 ccgtggccga tcggcacgtc tgcttctcct tttacagcga accccattcg ttcgtagaat
    11401 tgacgcgcct tgttgtaccg gttgacattc agccttagcg tcttcgcacc ctccggaccg
    11461 atctcgccga aaataaattg caggaccgat cttccaagac ctttcccctg ttgtcccggg
    11521 aggaaataga ccttatggag ctggtagacg ccaggttcgg cagatgcgga ccaggaagcg
    11581 aaaccgatgg cttcttcgcc ttgttccatc aggacgaagc tgtgatgttc gtcgaccatt
    11641 tgccggcgca atgaggcggg gctatagaac atgttgagca tgtacttcag ctgttcggcg
    11701 gacaggatct taccgtaggt gtccggccat atctgctggg ccagaaagcc gatcgtattg
    11761 atatcgtcca ggtcggccgg tcttatcgat attcgttcat ccatcgtgtt cacggttttg
    11821 tcggttgaaa gtttgttcat cctcttcttc ttttgcgatc ttgtgcatcc gcaacgcgcg
    11881 taaggccagc cgtacggcga tatagaaagt cgcgaacgcc ccgaacggga gtactccgtt
    11941 ggagaggtac ctggtcactt catggttctg ctggaggaag agaaaaaaat cgaagcttcc
    12001 atggaacagc accgcgatgg ccagtccttt tgtcatcagc aggaaggagc ggtgtcgttc
    12061 gaccctggct cgtcccactg aataacccat caggacggcg aaggcggcat gcgctgggat
    12121 ggccagaaaa aagcgcgaaa cgccggtctc cagaccgaac tgccgtacat attcgatatt
    12181 ctctaccgtg gcgaaaccca tcccgatggc cacacaatag accacgccgt caaaaggctc
    12241 gcggaacatc tctttaggat atgcatagaa ccgcaggacc aggaacttgc tgccttcctc
    12301 gctgaaggct accaccgcaa aggcatacca aatataggac agaatggaat gacgccaggg
    12361 ttcattgcgc acatccaccg atagccactg gatcgccagc gggggaagcg tagccaacgc
    12421 gccgagtaaa aaagaaacga tcatgatacg catggatgcc ttccccatgt tgtggagaga
    12481 ccagatgaac agacagatgg cgatacccgg ggcgacagcc agcgcaagcg cgagtagata
    12541 catcccttaa aagtaggtta attgggtgag gcctgcgccg gtggcggggc cgtttggaag
    12601 aaatagatcg cgccgtcttt gaaatgtatc tcgcggatga gctgtttcct gctcttcagg
    12661 aagtcgacat cgatgagatc ggtattgacc gcatcgtcga accagatgac ggtgaacctg
    12721 tcttccttca acatataccg gatgtcctcg gcattgtctt tatgcggcag gaggtccgaa
    12781 cgtacacccg tcaggaacca gagtccttcg aacgcgtccg aataaagggt ccccagacgc
    12841 ggaatgtttc gcagcgaatc ctggtgatcg cggacgaagg ccatcgtttc cgaattgcgc
    12901 cagtcatttt cggtatagcc tgggatcccg gcatcatgta taccatccca gttctcccgg
    12961 aaggtctgca tctcgccggt aaagaaacaa atggcggccg caaggcaaag cagaatggcg
    13021 gcctttttcc accggggaga ggagggcgtc ggggtaccgg gtttaagttg gtgggtgccg
    13081 ggcgtcgcgg attccgattg cgtcccgggt ttaagccgaa gggcgcctgg tatccagcag
    13141 gtgcttcccc acagccagga caggaatagc ggagacaaca gccggctgtc caactcc
//
LOCUS       DASYSV010000330         7745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2495271, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000330 DASYSV010000000
VERSION     DASYSV010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2495271"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..580)
                     /locus_tag="VGQ51_09885"
     CDS             complement(29..580)
                     /locus_tag="VGQ51_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV9036922.1"
                     /translation="MSKITFLLAAGLLVFSTAVTAQPNAPKKPAPTLRSILLAQFKTT
                     DDQADWFVPVMKSIQGITAQQAMWKPNDSCHSIGQLVYHIWFWNKQSLDKFYGRTPDK
                     FDGNNDETFNDFTEASWASTVANLGQVMHDWEKAIGEADEAKLQTYYSTIAHINTHTA
                     YHTGQILYVRKLAGNWDSSKGVH"
     gene            complement(609..1175)
                     /locus_tag="VGQ51_09890"
     CDS             complement(609..1175)
                     /locus_tag="VGQ51_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9036923.1"
                     /translation="MKTNALNGNSAVIFQNIFQTLTGPSGAYQTIIGVRNIVGTGQYH
                     TFFASPANSDFSIRGGGSGSTYTDGPNANDWTNRSWYIGTFGRNSSGSVTLNFNFSDY
                     NGSTPVSTNTFALLYNPTSRYRRQYPIFADQDFGNRIIDVQGRIVSRMADAPGSVVNI
                     PVDGLQKGVYFAEVLSGQSKFIRTFIKN"
     gene            1524..4757
                     /locus_tag="VGQ51_09895"
     CDS             1524..4757
                     /locus_tag="VGQ51_09895"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV9036924.1"
                     /translation="MTFFRFGANAQGTRLLRQPSLSATQLAFEYGGDIWVVDKNGGEA
                     RRITSTPAVESDPHFSPDGRWIAFTSNRNGFATVYVVSAEGGIPKRLTWYPAAASARG
                     WTPDGKRVLYSTPRGTAPVGYGRLWSVAVTGGPSELLPAPWGWDGSLSADGKKIVIDR
                     VSRWDVEWRHYRGGQNTPLEILDLASLTEQPIPNPDRTMDIHPVWQGDAIYFLSDRDF
                     IMNVWTSPAGGGALRQVTSYKEGDIKWLTGNGKELAFERGGYLFLLDPSNGKTTQVNI
                     TVHGDFPWAEPHWENVTTRFEDVSLSPTGKRILGEARGDIFTIPVENGNARNLTHSSD
                     VADRRPLWSPDGRQIAWFSDKGGDSYALYITDQEGSREPRKIAIGESKLAWEPTWSPD
                     GKYIAFDDNKARVRIVEVATGKITTADDGGVNNERGGMGLAWSPDSKWLVYSKTAANN
                     FHRVTIWSVTTEHATALSDPLADASEAVWDRDGRHLYFLASTEVGLASGWANTSTILS
                     RPNFAVYVTVLRKDDPTPFPLKSDEEPDTVASTKSGNTAGVDKTGAKPDTTAKTGGKK
                     DAATVLIDFENIDRRTLPLPVPVGNYQSLRSGPKGSVFILSGDGNIGKFTLETKKLDE
                     FVTHAGNVHLSANGEKMLFRSGNTWKVVSTAKPPSPTEGTVAVNIRMELNRLDEWKQI
                     FTEGWRYERDYFYDPGLHGRNWDDVWKYYSPQLPYIRHRADLTYLLDQLGGELSVGHS
                     FVFGGDFPSVDTIRASALGADLVARDGRWQIRRIYTAESWNPELKAPLAQPNLKVSEG
                     DFVLAVDGQPLTAEQDPYELLDGKAGVQTLLLVNSKPSVEGAWTIKVEPVAGEGPLLQ
                     RAWVEDNRRKVDELSHGKLGYIWVPNTGGPGFVSFNRYFFAQQDKQAAVIDERFNGGG
                     LLDDYMVDLMIRRLRAAVTNEAPGGHAFRLPAGILGPKVLLINELAGSGGDFFPWIFR
                     HQQVGPLIGTRTWGGLVASAVHYSFVDGGAMTAPVNAVFDPIGHKWIAENQGVPPDIE
                     VKVDAVSVSHGRDPQLERAVEECLRLLDKEPTPSVEHPPYSRPGVKP"
     gene            complement(4831..6756)
                     /locus_tag="VGQ51_09900"
     CDS             complement(4831..6756)
                     /locus_tag="VGQ51_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEV9036925.1"
                     /translation="MFIRLIAALLIAAPLFLTAAPRPTTAHNPASAHSPVTAHYPAAP
                     WPSVHIIPQPVSVKQNPGHFTISPDTRIITEGDSLSEIASWLGDRLHTGKNGRGKQRI
                     ILHIGTGAATSVSAATTDATREGYTLAVTPTTIRINANTAAGVFYGMQTLEQLVSPGG
                     EIPCVDITDYPRFGWRGLMLDVSRHFFTKQEVEKYIDEMVRYKFNTLHLHLSDDQGWR
                     LEIKSLPALTQHGAWRVARTGRWGEFLPPLADEPTPYGGFYTQDDMRDIIRYAAEHYV
                     TILPEFDIPAHSLALISAYPNLSCTQLQYPVNPGSRGPIRQDNALCVGNDSVYAILDK
                     IFTEVAALFPNPYIHIGGDEAYKGFWAACPKCNKRMQEEHLKNVDELQSYFVRRIEKI
                     LQSKGKKLIGWDEILEGGLAPEATVMSWRGMSGGIAAAKMGHKVVMTPWDYTYLDLYQ
                     GEPSAEPPTYGMCRLKDSYTYDPVPDSVDEHLILGGQGNLWSESVPNFRHAEYMTWPR
                     GLALAEVYWSPKSARNWDDFTKRMESEFLRMDADNIKYARSAYNVVLTPKRIPGGDFE
                     LSMSTEVDGLDIYYTFDDTNPDATYPKYDGTPVRFPMGAAQINVITYRDGHPIGVQVN
                     VKKDDLAKRADQGHHVY"
     gene            complement(6767..>7745)
                     /locus_tag="VGQ51_09905"
     CDS             complement(6767..>7745)
                     /locus_tag="VGQ51_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEV9036926.1"
                     /translation="HVFFGADQESDYAGGTEDIIPDDFAPREGVTTVVDAGSSGWRNF
                     DVFKKRVIDHSATRVLAFLNIVGAGMRGGPYEQNTADMSDSLAAAKAGQYPAIIVGFK
                     VAHYTGGDWRPVDAATHAGNQTHLPVMVDFGEHLPELPIHDLFLKHLRPGDIFTHCFA
                     QLSDREPIVDPITKKLKPFVFEARTKGILFDVGYGEISFAFSQAIPALHSGFPPNSIS
                     SDNHRSNIRVTLPDIMSRFLALGLDPATVIGMVTWKPAQEIHRPELGHLSEGAVADIV
                     ILRMQHGRFTFSDHAGRRIEAAQRFECMMTIKGGKVEYDRFIPKFTAHS"
BASE COUNT         1622 a         1977 c         2388 g         1758 t
ORIGIN      
        1 gcggcatcgt cgtactggaa taccggcgtt agtgcactcc cttagaggaa tcccaatttc
       61 ccgccagctt gcgaacatac aatatctgcc cggtgtgata ggcggtatgc gtgtttatat
      121 gcgcgatagt agaatagtag gtctgcaatt ttgcctcatc ggcctcgccg atcgcctttt
      181 cccaatcgtg catcacctgt ccgagatttg cgacggtgga agcccatgag gcctcggtga
      241 aatcgttgaa ggtctcgtcg ttgttgccat cgaatttgtc gggcgttctt ccataaaatt
      301 tatccagcga ctgtttgttc cagaaccaga tgtgatagac cagctgtccg atcgaatgac
      361 aggaatcgtt tggtttccac attgcctgct gcgcggtgat accctggatg ctcttcatga
      421 ccgggacgaa ccagtcggcc tgatcgtcgg ttgtcttgaa ttgtgcgagc aggatagacc
      481 ggagtgtggg cgccggtttc tttggcgcat tgggttgggc cgtgacagcc gtcgagaaca
      541 cgaggagacc cgcggcgagg aggaaggtga tctttgacat ggttaagcag ttgtgtggtg
      601 aaggatgatc agttctttat gaaagtacgg ataaatttcg attgtccgga aagcacttcc
      661 gcgaaataga cgcctttctg cagcccgtct acaggaatat tgacgacact gccaggcgcg
      721 tcggccatcc ggctaacgat cctgccctgc acgtcgatga tacggtttcc aaagtcctga
      781 tcggcgaata tcggatactg ccgtcgatat cgactggtag ggttatataa cagggcaaag
      841 gtgtttgttg aaacgggagt gctgccgttg taatcggaga aattgaaatt gagcgttacc
      901 gacccggagg agttgcggcc gaatgtcccg atgtaccagc tgcggtttgt ccaatcgttg
      961 gcattcggac cgtcggtata ggtgctaccg gacccgccgc cgcggatgct gaaatccgag
     1021 tttgcgggac tggcgaaaaa ggtatgatat tgtccggtgc cgacgatatt cctaacgccg
     1081 atgatcgtct gataggctcc cgagggcccc gtcaatgtct ggaaaatatt ctgaaagatg
     1141 actgcggaat tgccgttgag agcgttcgtc ttcaaaacag gtcgcgttag cgcgaaggtt
     1201 tgcatagcgt cttgcgcgtg taaaggaagt tcatcggata ctgaaatttt agctgagata
     1261 tcttatgcca ggaccaagct aagagataac taattgattt tcaataggca agggtaaatg
     1321 aatagtctta aatttggaat gttttctagt ttttggatgt tatcccaaaa tgagtatcct
     1381 ggggtctgag taggccgcgc ggggttacgg agatagctcg cgtatatcgt tatgcgggaa
     1441 ttgtcgtaca ttttactatg ccaaacctaa actccctccg actccccggc ctcatcgtga
     1501 ggctagtttt tttcagttgt cttttgactt tttttcgctt tggcgcgaat gcgcaaggaa
     1561 cccgtctgct gcggcagccc agcctcagcg ctacgcaact ggcctttgaa tatggtggcg
     1621 atatctgggt cgtcgacaag aatggcggcg aagcccgtcg tatcaccagc acgcccgcag
     1681 tagaaagtga tccgcatttt tcgcccgacg gccgatggat agcctttacg tccaaccgca
     1741 acggtttcgc tacggtatat gtcgtatcgg cggaaggtgg gataccgaaa aggctgacct
     1801 ggtatcctgc tgctgccagc gcgcgcggat ggacgccgga tgggaaacgc gtgttgtaca
     1861 gcactccgcg cgggacggcg ccggtgggtt atggccggtt gtggagcgtc gccgtgacgg
     1921 gtggcccgtc cgagttgttg cctgctccat ggggttggga tggatcgttg tcggcggacg
     1981 gtaagaagat cgtcatcgat cgcgtcagcc gttgggatgt cgagtggcgt cactatcgcg
     2041 gaggccagaa tacgccgctg gaaattttgg atctcgcgtc gttgaccgag cagcccatac
     2101 cgaatccgga tcgtacgatg gacattcacc cggtgtggca gggggatgcg atatactttc
     2161 tatcggaccg cgatttcatc atgaacgttt ggacttcgcc tgccggcggt ggcgccttgc
     2221 ggcaggtgac gtcctataaa gaaggagata ttaaatggct gacgggcaac ggaaaggaac
     2281 tggcattcga gcgcggtggt tatctgtttt tattggaccc ttccaatggg aaaaccacgc
     2341 aggtgaacat cacggtgcac ggcgatttcc cctgggccga accgcactgg gagaacgtca
     2401 ccacccgttt tgaagacgtg tcgctatcgc ctacgggcaa acgcatcctt ggtgaagcgc
     2461 gcggtgacat ctttactatt cccgtggaga acggcaatgc tcgcaacctc actcatagca
     2521 gcgatgtggc cgaccgcaga ccgctctggt cgccagacgg ccgacagatc gcctggttct
     2581 cggataaggg tggagacagc tatgccttat atatcaccga ccaggagggc agccgcgagc
     2641 ctcggaaaat tgcgatcggc gagtcgaagc tggcctggga acccacctgg tcgccggacg
     2701 gaaaatatat cgccttcgat gataataagg ccagggtccg gatagtcgag gtcgccacgg
     2761 gcaagatcac gacggccgat gacggcggag tcaacaacga aagaggcggg atggggctgg
     2821 catggtcgcc cgattccaaa tggctggtat attcgaaaac ggccgcaaac aattttcacc
     2881 gcgtgacgat ctggtccgtg acgacggaac acgccaccgc gctctccgat ccgttggccg
     2941 atgccagcga agcagtatgg gatcgtgacg gaagacatct atatttcctg gcaagtacgg
     3001 aggttggttt ggcttccgga tgggccaata ccagcacgat actctcgcgg ccgaatttcg
     3061 cggtttatgt aaccgtcctg cgcaaggacg atccgacgcc cttcccgctg aagagcgacg
     3121 aggaacccga taccgtggcg agtacaaaat cggggaatac ggcaggtgtg gataagacgg
     3181 gtgcaaagcc ggatacgacc gcgaagaccg gcggcaaaaa ggacgcggcc accgttctga
     3241 tcgatttcga gaatatcgac cgccgcactt tgcccttacc agtacctgtc ggcaactacc
     3301 agtcgctacg cagcgggccc aaaggatcgg tattcattct ctccggcgat ggtaacatcg
     3361 gcaaattcac gctcgagacg aaaaagctcg acgaattcgt cacacatgcg gggaatgtcc
     3421 atctatcggc caacggcgag aagatgctgt tccggagcgg caacacatgg aaggtcgttt
     3481 caaccgccaa gccgccgtcc cctacagaag ggacggtggc ggtcaatatc cggatggagc
     3541 tcaacaggct cgatgaatgg aaacagatct ttaccgaggg ctggcgttat gagcgcgatt
     3601 atttttacga tcccgggctg catggcagga actgggacga cgtatggaaa tattattcgc
     3661 cgcagctgcc ttatatccgg caccgcgccg acctcactta tttgttggat cagttgggtg
     3721 gcgagctgag tgtcggtcac agctttgtct tcggcgggga ttttccttcc gtagatacga
     3781 tacgtgcgag cgctctcggg gccgacctcg tcgcgcgtga tggacgctgg cagatccggc
     3841 ggatctatac ggccgaaagc tggaatcccg aattgaaagc gccgctggct cagccgaatt
     3901 taaaagtctc ggagggcgac tttgttctgg ccgtggacgg gcagccgctg accgcggagc
     3961 aggaccctta tgaattgctc gatggtaaag cgggtgtgca aactttattg ctcgtcaaca
     4021 gtaaaccgtc ggtggaaggg gcatggacga tcaaggtcga gcccgtcgcg ggagaaggcc
     4081 cgctgcttca gcgggcatgg gtcgaagaca atcggcgaaa agtagacgag ctctctcatg
     4141 gaaagctggg ttatatatgg gtaccgaata caggggggcc gggattcgtg tctttcaacc
     4201 ggtatttctt tgcacagcag gataagcagg cggcagtcat cgacgaacgg ttcaatggcg
     4261 gaggcctgct ggacgactat atggtcgacc tgatgatacg gcgtttacgc gccgccgtta
     4321 ctaacgaggc gcccggtggc cacgctttcc gtcttccggc gggcatcctg ggaccgaagg
     4381 tgctgctcat caacgagctg gccggatcgg gcggagattt ctttccatgg atattccggc
     4441 atcagcaggt gggccctctc atcggtacga ggacatgggg cggactggtg gcttccgccg
     4501 tccattatag cttcgtcgac gggggcgcga tgacggcgcc ggtcaatgcg gtattcgatc
     4561 ccataggaca taaatggatc gccgaaaacc agggcgtacc tccggacatc gaagtaaaag
     4621 tcgatgccgt atcggtctca cacggtcgtg atccgcagct ggagcgcgcg gtcgaggaat
     4681 gtctgcggtt gctggataag gagcctacgc cttcggtaga gcatccgccc tattcgcggc
     4741 cgggtgtgaa gccttaacaa tgtcgcgacg ggcgtgtcgt gtgggctccg gtgtggtctc
     4801 gcggtgtggt ctcgcggcgc gcctggtccg tcaatataca tggtgcccct ggtcggcgcg
     4861 tttagcaaga tcgtcttttt tgacgttgac ctgcacgccg atagggtggc cgtcgcgata
     4921 cgtgatcaca ttgatctgtg cggcgcccat cgggaacctg actggtgtgc cgtcatattt
     4981 cggatatgtg gcatcgggat tcgtatcatc gaaggtatag tagatgtcga gcccgtcgac
     5041 ctccgtcgac atgctcagct cgaaatcgcc gccggggatg cgcttgggag tgagcacgac
     5101 attatacgcg ctccgggcat atttgatatt atcggcatcc atgcgcagga attcgctttc
     5161 cattctcttc gtgaaatcgt cccagttgcg tgccgacttg ggactccagt agacttccgc
     5221 cagcgcgaga ccacgaggcc aggtcatgta ttctgcatgc cggaagttgg ggacggattc
     5281 cgaccacaaa ttgccctgtc cgccgaggat gaggtgctcg tcaacgctgt ccgggacggg
     5341 atcataggta tagctgtcct taaggcggca catgccatat gtgggtggct ccgccgaagg
     5401 ctcgccctgg tagaggtcga gataggtata atcccaggga gtcatgacta ccttgtgtcc
     5461 catctttgcc gcagcgatac cgccggacat cccacgccag ctcataacgg tcgcctcggg
     5521 tgcgagaccg ccttcgagga tctcgtccca gccgatgagt ttcttgcctt ttgattgcag
     5581 gatcttctcg atccgccgga caaaataact ttgcagctcg tcgacatttt tcaggtgctc
     5641 ttcctgcatc cttttattgc attttggaca ggcggcccaa aagcccttat atgcctcgtc
     5701 gccgccgata tggatatacg ggttcgggaa aagtgcggca acttcggtga atatcttatc
     5761 gagtatcgca taaacactgt cattgccgac gcaaagcgca ttgtcctgcc gtatgggtcc
     5821 gcgcgatccg ggattcaccg ggtattgcag ctgggtacag gaaagattgg gataggcaga
     5881 gatcagcgcg aggctatgtg ccgggatgtc gaattcggga aggatggtaa cataatgctc
     5941 cgcggcatac cggatgatat cgcgcatgtc gtcctgcgta tagaatccgc catatggtgt
     6001 cggttcgtct gccagcgggg gaaggaattc accccaccgc ccggtccttg cgacgcgcca
     6061 tgccccatgt tgcgtgagtg ccggcaggct tttaatctcg agacgccaac cctggtcatc
     6121 gctcaggtgc aagtgcaggg tgttgaattt atagcggacc atctcgtcga tatatttttc
     6181 cacctcctgt ttggtaaaga agtggcggct cacgtcgagc atcagtccgc gccagccaaa
     6241 tcgggggtag tcggtgatgt cgacgcaggg gatctcgcct cccggcgaaa ccagctgctc
     6301 cagggtttgc atgccataaa atacgcctgc ggcggtattg gcgttgatgc ggatggtggt
     6361 gggtgtgacg gcaagggtat atccctcgcg ggtggcgtcc gtggtggctg ccgagacgga
     6421 ggtggctgcg ccggttccga tgtggaggat gatgcgttgt ttaccccgac catttttgcc
     6481 ggtatgtagc cggtcgccta gccaggaggc gatctcgctc agcgaatcgc cttcggtgat
     6541 gatccgggtg tcaggagata tagtgaagtg accggggttt tgtttgacgg acacgggctg
     6601 cgggatgatg tggacggagg gccatggcgc ggctgggtag tgggctgtga ctgggctgtg
     6661 ggctgaggct gggttgtggg ctgtggttgg gcgcggcgcg gcggttagga acaacggcgc
     6721 ggcgatgagg agggcagcga tgagacggat gaacatggcg atctatttag gaatgagccg
     6781 tgaatttagg aataaatcgg tcatattcga cctttcctcc tttgatcgtc atcatacatt
     6841 cgaatctttg ggctgcctcg atacgacgtc cggcatgatc cgagaaggta aatcgcccgt
     6901 gctgcatacg caggatcacg atgtcggcga cggcgccttc ggatagatgg cccagttccg
     6961 gccgatggat ctcctgcgca ggcttccagg tgaccatccc gatcaccgtg gcaggatcga
     7021 ggcccagcgc gagaaatcgg gacatgatat ccggtagcgt gacacggata ttactgcgat
     7081 ggttatccga actgatcgag ttcggtggga aaccgctgtg cagcgccggg atggcctgcg
     7141 agaatgcgaa actgatctcg ccatagccga cgtcgaagag aatgcctttc gtccgggctt
     7201 cgaagacgaa gggttttaat ttttttgtga tggggtcgac gatcggctcg cggtcgctca
     7261 gttgggcgaa acaatgtgtg aatatgtctc cggggcgaag atgtttcagg aatagatcat
     7321 ggatcggcaa ctcgggcagg tgttctccga aatcgaccat gacgggaaga tgtgtttgat
     7381 taccggcatg tgtcgcagcg tcgacgggac gccagtcgcc gccagtataa tgagctactt
     7441 tgaagccgac gatgatcgcg ggatattgcc cggccttggc agcggcgaga ctgtcgctca
     7501 tatcggcggt gttttgttca tagggcccac cgcgcatacc cgcgccgaca atattgagaa
     7561 aggccagcac ccgggtagcg gagtgatcga tgactctttt tttgaagaca tcgaaattgc
     7621 gccatcccga gctgcctgcg tctacgaccg tggtgacgcc ttcgcgggga gcgaaatcat
     7681 cggggatgat atcctccgtc ccgcccgcat aatcgctttc ctggtcggca ccgaagaaca
     7741 cgtgc
//
LOCUS       DASYSV010000331        10160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2368611, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000331 DASYSV010000000
VERSION     DASYSV010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10160
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368611"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2254
                     /locus_tag="VGQ51_09910"
     CDS             <1..2254
                     /locus_tag="VGQ51_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9036927.1"
                     /translation="TIAVYQIPGSNAIAVAEAVQKQMEALKARFPQDMKLSYALDTTL
                     AVHAGIEEIVHTLFEAVVLVILVVFLFLQNWRATLIPLLTVPVSLIGTFIVFPLLGFS
                     VNTLSLLGLVLAIGLVVDDAIVVVEAVIHNIEHGMAPKEATSKAMKEVGGPVVAIAVI
                     LSAVFIPVALAPGIVGRLYQQFAITIAISVLFSAFNALTLSPALAALLLKPASGKKGL
                     LDRFFAAFNRVFGRLTNGYVKVAGFFAKRMIVTLLVLSGIVVVTISLGKRIAGGFVPE
                     EDQGYFIVSVQLPDAASLERTDKVTRKVEGLLRQVPEIDLYTTVNGNSLLNNTVAPNA
                     ATFFITLKPWAERKRTANDIVAQINALAVRNISEAIVLAVGPPPIVGLGNAAGFTLML
                     QDRAGNSPQYLAEQAQRFIAAASKRPEIGRIFTLYRANVPQKSIGVDKEKVGKLGIPL
                     DDVNSTISTLLGGYFVNNFNLFGRQYKTYVMADARYRMTPEDVQRFYVRDPKGNPVSI
                     GTITQILDTSGPQYTNHFNIYRAAEIGGAPAPGYSSAQATNALEAVAKEVLPSDMGYQ
                     WSNVTYQEKSAEGSAGAIFGLALFFVFLILAALYESWKLPFSVLLGTPWAAMGAFLGL
                     FLGAMYSASYVNNVFAQIGLVMLIGLNAKNAILIVEFAKMNKDAGNETVFSALEGARM
                     RFRPILMTSLAFILGVTPLLTAHGAGAEARKVMGMTVFSGMLIATIIGVILIPSFFVL
                     IEGKRKKKAT"
     gene            2251..3648
                     /locus_tag="VGQ51_09915"
     CDS             2251..3648
                     /locus_tag="VGQ51_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9036928.1"
                     /translation="MKLLPIAAGCAFSVLLSGGCMVGPKYTRPAVDVPAVYSQASNPD
                     TLALVKWFDLFHDTVLQQIIHTTLQNNRDLLTTTARIDETRFIVSIARANIYPALNYN
                     ITGGGGKAGSDAEKVAAGFNSGYLNAGVQLNWEVDLWGRLRRAKRASQRQLMAETDNR
                     NAVLTSLVAQTATTYFLLLDLDNQLLISRRTLESRKERTRINMARFEKGYSSEIDMLQ
                     AKQQEAVAAASTAGLQRQIIAVENNLRLLMGEGPGHIARGDSLYHQVLPPDIPTGLPS
                     QLLRRRPDIRSAEEVLQAQFEQIGIAQANLFPTLSLTGVLGFASPQLSSLVSSNGFVA
                     NGFAGLFGPLFQFNRNKDLVGAAKERTKQAAYQFEQTVLIALGEVDNALADARNLASE
                     YEYLDQQVDASARALALTVARYDYGYSSYTEVLIEENSLFDAELLASSVYQQRLAAIV
                     NLYRALGGGWEVDSF"
     gene            3681..4169
                     /locus_tag="VGQ51_09920"
     CDS             3681..4169
                     /locus_tag="VGQ51_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEV9036929.1"
                     /translation="MHSRGAMGAFAQCNTQLKLIESKMEEEKQQIDNRFLNEINQQKE
                     TMDEQVRLLQVYGQQYKDSWKGKSLGLIHGKIGFRTGNPKLIKDRKFTWDGVTELLKK
                     AFPSFVRTSYEINKEALIAFRDEKDFEKVRESCYVDVVQDESFYVEADAEELSVAYSI
                     HR"
     gene            4325..4819
                     /locus_tag="VGQ51_09925"
     CDS             4325..4819
                     /locus_tag="VGQ51_09925"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEV9036930.1"
                     /translation="MIRTVLLVVLVLFIAIQFFRPSKNNSGDTHKSITQLYEVPPNVD
                     AILQRSCRDCHSNKTNYPWYDEIQPVAWWVNGHIGNGKRHLNFDTYSAQRIAIQKKRM
                     EDCLEQLKNDEMPLSSYTLIHRNAILTQADKDTLSGWCQGIIDQIKAKYPPDSLVLPK
                     RKKP"
     gene            4822..5856
                     /locus_tag="VGQ51_09930"
     CDS             4822..5856
                     /locus_tag="VGQ51_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEV9036931.1"
                     /translation="MSIKSSGTISRFAAGLLPAVLVASSALAQPTPALSLLALSKADH
                     TLAIVDPVSLKVIAKMPVGTDPHEVIASSDGRTAYVSIYGGGSLHEMDVLDLVSKKAL
                     TPVDTRPFFGPHGIEFAGGKVWITAEGSKCVARYDPATHAFDWVMGTGQDRTHMIYVT
                     HDEKTVYTTNVSSGTVSILTDTLMRGSPPGAPPHEDWQQTLIPVSRGSEGFDVTPDGR
                     QLWTASAGDGTISVIDLATRQVVDKIAAGVNGANRLKFTPDGKMVLITSLGNGDLVVY
                     DATTRKEAHRIKIGRGAAGILVSPDGTRAFLGCTPDNYVAVVDLRTLQVLGHIDVGGG
                     PDGLAWAIRQ"
     gene            complement(5913..6287)
                     /locus_tag="VGQ51_09935"
     CDS             complement(5913..6287)
                     /locus_tag="VGQ51_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036932.1"
                     /translation="MYKILLFISLFLFTTALSAQIIGRVDKRTKGFFIPADQQGKYLV
                     FGFQFPSAATKKMICFSNSADNLRANYQCPLGAYFDTGQMGPGDNIIFLGMYSTFGKM
                     VYNAGNGTKTIFYLPKTSFTIK"
     gene            complement(6489..6647)
                     /locus_tag="VGQ51_09940"
     CDS             complement(6489..6647)
                     /locus_tag="VGQ51_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036933.1"
                     /translation="MSNRTTIVTLTAQPEIIAALADLLIETVANHGSIGFMQSVTRIS
                     RIARRSLK"
     gene            complement(6617..6838)
                     /locus_tag="VGQ51_09945"
     CDS             complement(6617..6838)
                     /locus_tag="VGQ51_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036934.1"
                     /translation="MNTVTIDTVFSAPAKDVFPDRMIQGPATGKTRHPGLHYFNSLEW
                     LQFAEMHMRHHFRQKKRIDACLTEPPSSR"
     gene            complement(6835..7293)
                     /locus_tag="VGQ51_09950"
     CDS             complement(6835..7293)
                     /locus_tag="VGQ51_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV9036935.1"
                     /translation="MLYLVEKYAGRDIAVSCAKGFQIDLQRSSQSPFIIFSGQKDHSD
                     LAIKKAQEFIERNVHSRVSVDALASLAALSRRNLERRFKKATGNTVMEYVQRVKMEAV
                     KKNLESSRLGVNEAMDKVGYSDPKAFRMIFKKVTGLSPVQYRNRYNREYV"
     gene            complement(7299..7811)
                     /locus_tag="VGQ51_09955"
     CDS             complement(7299..7811)
                     /locus_tag="VGQ51_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV9036936.1"
                     /translation="MIHVSILVPEGNCSLTNIEGTQQILMRVNGYLEDAGRDALFDVH
                     LVGHLKQVNMKNGMFMVCPDQLIGEVARTALIIIPSVHGDKQKIVADNHALLSWIVEQ
                     YKGGAAIASLCIGAFLLAGTGLLDGKSCTTHWEMADDFRKLYPAVHLLEEKIITDNDN
                     IYTVEAHIPG"
     gene            complement(7838..8968)
                     /locus_tag="VGQ51_09960"
     CDS             complement(7838..8968)
                     /locus_tag="VGQ51_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEV9036937.1"
                     /translation="MQRIAIPPRNNWRSTVEQLGFGFHTTNVPYWDESAYYSFSTDEI
                     LLIEEATTQLWELCLQAVQYTIDHRLYDLFHIPRSFVPCLEESWREDHPSIYGRMDLC
                     YRDGKVKMLEFNADTPTSLFEAGIVQWFWLKDFHPAKDQFNSIHEKLIEHWTGLQPYL
                     YPGTLHFACLKDTLEDLTNVEYLRDCAIQAGIPTHFLFVEDIGWDEKQQVFVDDQQIA
                     IKNLFKLYPWEWLMADEFGEHIGRDKNQAFFIEPAWKMILSNKAILPLLWELFPGHPH
                     LLPAYFSSEPLTNYVKKPILSREGANIAIMVGKDLVGRTDGDYGEEGYICQEFFPLPA
                     FGDNYPLIGSWVIGQQPAGIGIREAGTLITDNLSRFLPHLIE"
     gene            complement(8989..9426)
                     /locus_tag="VGQ51_09965"
     CDS             complement(8989..9426)
                     /locus_tag="VGQ51_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036938.1"
                     /translation="MKKTKTIHLVLITAALASCNRVIIPGQSAEVSGTDSALTAATAE
                     EDSVYDCGCQPVSNNYNGYNPYDYYNNPFFNFNIFYTGRPFNVPYRAAPRYPINTSFR
                     NHTFVVRGGFGKAASAGSSAASAASSAASAGSSAASAGSSAAS"
     gene            complement(9432..>10160)
                     /locus_tag="VGQ51_09970"
     CDS             complement(9432..>10160)
                     /locus_tag="VGQ51_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036939.1"
                     /translation="SWAQEIRKDHPLDTTGSFICRTVSGDIADPKALLKAFPESCPRI
                     AQAEKNLQKYIDLGFMNCRDAHTGTAAVMMGWWFPTDHLSQVLGSHPSVGVQLGYRTP
                     RNEYDITWNVRFLYPTPRPYTFLRQDTVYTSNYYDGGYIGFEYSRYFVHKKHVDLGYT
                     AGIAYDYFSVADGWSDHHRAVNITPLNVGSFDLNYGLRFKYFLHCHAFLGLTAKYHII
                     HYNNDGGTDLSGNAFTLDLLFGSN"
BASE COUNT         2262 a         2671 c         2903 g         2324 t
ORIGIN      
        1 gacgattgcc gtctaccaga taccggggtc caatgcaatt gcagtggctg aggcggtgca
       61 gaaacaaatg gaagccctga aagcccgttt tccgcaggac atgaagctct cttatgcgct
      121 ggatacgacg ctggcggtcc atgcaggcat cgaggagatc gtccacaccc tgttcgaggc
      181 ggtcgtactg gtcatcctcg tcgtatttct tttcctgcaa aactggcggg ccacactgat
      241 cccgctgctc accgtacccg tttctctgat cggcaccttt atcgtcttcc ccctcctggg
      301 tttttccgtg aatactcttt ccttattggg attggtcctt gccatcggcc ttgtagtgga
      361 tgatgcgatc gtagtggtcg aagccgtcat ccacaatatc gaacatggca tggcgccgaa
      421 ggaagcaacg agcaaggcta tgaaagaagt aggcggaccg gtggtagcca ttgcagtgat
      481 cctttcggct gtattcattc cggtcgcact tgctcccggg atcgtcggcc ggctttacca
      541 gcaatttgcc atcaccatcg ccatctcggt attgttttcg gccttcaatg cgctgaccct
      601 cagcccggcc ctcgctgctt tgttactcaa acccgccagc gggaaaaaag gactgctgga
      661 caggttcttt gcagccttta accgggtctt cgggcggctg actaacgggt atgtaaaggt
      721 agccggattc tttgcaaaaa gaatgatcgt caccttgctg gtgttgagcg gtatcgtggt
      781 tgttacgatc agtctcggca aaagaatagc gggcggcttc gtccctgaag aagaccaggg
      841 gtatttcatt gtcagcgttc agctcccgga tgcggcgtcg ctcgaacgca cggataaggt
      901 cacccgcaaa gtagaaggct tgcttcgcca ggtcccggaa atcgatctgt atacgaccgt
      961 caatggtaac agcctcctga acaataccgt cgccccgaat gcggcaactt ttttcattac
     1021 gctcaaaccc tgggccgaac gcaagaggac ggcgaatgat atcgtcgcac agatcaatgc
     1081 cctggccgtc aggaatatct ccgaagccat cgtactggcc gtggggcctc cgccgatcgt
     1141 gggattgggt aatgcagcgg gatttacgct tatgctccag gaccgggccg ggaattcacc
     1201 gcagtacctg gccgagcagg cacaacggtt catagccgct gccagcaagc gcccggagat
     1261 cggcaggatc tttacgcttt acagggctaa cgtcccgcag aaaagtatcg gggtcgataa
     1321 ggaaaaggtc ggcaagctcg gaatcccgct ggacgatgtc aattctacga tctccacctt
     1381 gctgggagga tatttcgtga ataatttcaa cttgttcggc cggcaataca agacctacgt
     1441 catggcggat gcccgttacc ggatgactcc ggaggacgtg cagcgtttct acgtgcggga
     1501 ccccaaagga aatcctgtct ctatcgggac gataacgcag atactggata ccagcggccc
     1561 tcaatacacc aatcacttca atatctaccg tgcggcggag atcggcggtg cgcctgctcc
     1621 cgggtatagt tccgctcagg cgacgaatgc cctcgaggcc gtcgcgaaag aggtcctgcc
     1681 gtcggatatg ggttatcaat ggagcaatgt cacttaccag gaaaaatccg cggagggcag
     1741 cgctggcgcg atcttcggtc tggccctgtt cttcgttttc cttattctcg ccgcactcta
     1801 tgaaagctgg aaactgcctt tcagtgtcct gctgggtacg ccctgggccg ccatgggtgc
     1861 cttcctgggt ttgttcctcg gagcaatgta ttccgccagt tatgtcaata atgtctttgc
     1921 gcagattggg ctggtcatgc tcattggtct caacgccaag aatgcgatcc tgatcgtcga
     1981 atttgccaag atgaacaagg atgccggaaa tgaaaccgtc ttctccgcgc tggaaggcgc
     2041 ccggatgcgg tttcgaccga tcctgatgac ctcgctggcc tttatcctag gcgtgacgcc
     2101 gttgctgacc gcccacggcg caggcgccga agcccggaag gtcatgggca tgaccgtttt
     2161 cagcgggatg ctgatcgcaa cgatcatagg cgtgatcctc atcccgtcct ttttcgtgct
     2221 catcgaaggg aaaagaaaaa agaaagcaac atgaaactcc ttccaattgc agcagggtgt
     2281 gctttttcgg tattgctgtc cggcggttgt atggtcggtc ccaaatatac cagaccggcc
     2341 gtcgatgtac cggccgtgta cagccaggct tccaatcccg acacgcttgc cctggtgaaa
     2401 tggttcgatc ttttccatga tacggtcctg cagcagatta ttcataccac gctgcagaat
     2461 aacagggacc tgctgacaac aacggcacgg atcgacgaaa cacgttttat cgtgagcatt
     2521 gccagagcca acatctatcc ggcgctgaac tataacatta ccggtggcgg tggaaaggcc
     2581 ggaagcgatg ccgaaaaggt agcggccggt ttcaacagcg ggtatctcaa tgcgggtgtc
     2641 cagctcaact gggaagtgga tctctgggga aggctccgcc gggcgaaacg ggcgtctcaa
     2701 cggcaattaa tggccgagac cgataacagg aatgccgtcc tgacaagcct tgtcgcgcag
     2761 accgccacta cctatttcct gttgctcgat ctcgataacc agctgcttat ttcccggcgg
     2821 accctggaaa gccggaagga acgcaccagg atcaatatgg cccgcttcga aaaagggtat
     2881 agctcggaga tcgatatgct gcaggccaaa caacaggaag ccgttgccgc cgccagcacg
     2941 gccggtctcc aacgacagat cattgcagtg gagaacaatc ttcggctatt gatgggtgag
     3001 ggtccgggtc atatagcgcg aggggattcg ttgtaccacc aggtacttcc gcccgatatt
     3061 cccaccggcc tgccttccca gttgctccgg cgaagacccg atatccggtc ggcggaagag
     3121 gtattgcagg cccagttcga gcagatcggt atcgcgcagg ccaatctctt ccctacgctc
     3181 agcctcaccg gggtgctggg tttcgccagt ccgcaactaa gtagtttggt cagtagcaac
     3241 ggatttgtag ccaatggctt tgccgggcta tttggaccgt tattccaatt caacaggaac
     3301 aaggatcttg tcggcgcggc aaaagaaagg accaaacagg cggcctatca gtttgagcag
     3361 acggtactga ttgctcttgg cgaggtggac aatgcattgg ccgatgcgcg gaacctggct
     3421 tcggaatatg agtatctgga tcagcaggtg gatgcatctg cccgggcgct ggctcttacc
     3481 gtagcccgct acgactatgg atattcgtcc tataccgaag tgcttatcga ggagaattcg
     3541 cttttcgatg cggagctgct ggcatcatcg gtgtatcagc aaaggcttgc cgccatcgtc
     3601 aacctgtatc gcgcgctggg aggcggatgg gaggtggaca gcttttgatc atgggaagaa
     3661 ttcctgaaaa tgaatgaata ttgcactctc gcggagctat gggcgcgttc gcgcaatgca
     3721 acacccagct gaagctcatc gaatcgaaga tggaagaaga gaaacagcag atcgacaaca
     3781 ggtttctcaa cgagatcaac cagcagaaag agaccatgga tgaacaggtg aggctgctcc
     3841 aggtgtatgg ccagcaatac aaggatagct ggaagggaaa gagtctgggg ctcattcatg
     3901 ggaagatcgg tttccggacc ggcaatccca agctgatcaa ggaccggaag tttacctggg
     3961 atggcgtgac cgagctgctg aaaaaagcct ttccatcctt tgtccggacc agctacgaga
     4021 tcaataagga agcgctgatc gccttccgcg atgaaaagga tttcgaaaag gtcagggaga
     4081 gctgttatgt ggacgtcgtc caggacgaat cgttctacgt agaggccgat gccgaggagc
     4141 tgtcggtggc ctattctatc catcggtgag atggcggatc cccacgccga atcgccgcat
     4201 ccgcatccgc atcgctgagc atccgcatcc gcgttgctga accgccgcgt tgctgagcca
     4261 ccgcatgtgc cggaggctat tcgttatttt tcttatattc gcctccaacc aacccttcgc
     4321 atctatgatc aggaccgtgc tcctcgtcgt cttagtgctt tttatcgcaa ttcagttttt
     4381 ccgtccgtca aaaaataatt cgggggacac gcacaaaagt atcacccagc tgtatgaagt
     4441 tccgccgaac gtcgatgcga tcctgcagag gtcctgcagg gattgtcatt ccaacaagac
     4501 caactatccc tggtatgacg agatccagcc tgtggcctgg tgggtgaacg gccatatcgg
     4561 gaacggcaag cggcatctga atttcgatac ttattctgca cagcgcatcg ccattcagaa
     4621 aaaaagaatg gaggattgtc tggaacagct gaagaacgac gaaatgccgc tgagctccta
     4681 caccctgata caccggaatg ctatcctgac ccaggccgat aaggatacat tgagcgggtg
     4741 gtgtcaggga atcatcgatc aaattaaggc gaaatatccg cccgacagcc tggtattgcc
     4801 caaacggaaa aaaccatagt tatgtctatc aaatcatcgg gaacgataag tcgcttcgcc
     4861 gcgggcctgt tgccggctgt cttagtcgcc tcctccgccc tggcacaacc cactccagcg
     4921 ctgagcctgc ttgcgttgtc caaggccgac cataccctgg cgatcgtgga tccggtgtcg
     4981 ttgaaggtca ttgccaaaat gcctgtcggt acggaccctc atgaggtgat cgcttcttcg
     5041 gacgggcgca ccgcctatgt ttccatctat ggtggcggta gtcttcatga gatggatgtt
     5101 ctcgacctgg tctcaaaaaa agcgctgaca ccggtagata cccgtccttt tttcggtccg
     5161 cacgggatcg aatttgcagg cggcaaagtt tggatcaccg cggagggatc gaaatgcgtg
     5221 gcccgttatg accccgcgac gcatgccttc gattgggtga tgggcaccgg tcaggacagg
     5281 acacatatga tctatgttac ccacgatgag aagaccgttt atactaccaa tgtctcgtcg
     5341 gggacggtaa gcattttaac cgatacgctg atgcgtggca gtcctcccgg cgccccgccg
     5401 catgaggact ggcagcaaac gctgatccct gtgtccaggg gatcggaagg tttcgatgtc
     5461 acgccggacg gccggcaact ctggacggca tccgccgggg atgggacgat ctccgtcatc
     5521 gatctggcga ccaggcaggt ggtggataag attgcggcag gagtaaacgg cgccaatcgt
     5581 ctgaagttca ctcccgacgg caaaatggta ttgatcacca gcctgggcaa tggcgatctg
     5641 gtcgtttatg acgcgacgac acggaaggaa gcacaccgga taaagatcgg tcgcggagcc
     5701 gcaggcatcc tcgtcagccc cgacggtaca cgcgccttcc tgggctgcac gccggacaat
     5761 tatgtggcgg tcgtcgatct ccggacgctg caggtcctcg gccatatcga tgtgggtggc
     5821 ggacccgacg gtctggcatg ggcaatcaga cagtagatcg accgcggcag cgatccggcg
     5881 gcctccgcgt tttgcgcgtc agtctaccag tgctatttta tcgtaaaact ggtcttcggg
     5941 agatagaaga tcgttttcgt tccattcccg gcattgtaaa ccatcttgcc gaacgtgcta
     6001 tacataccca ggaaaatgat attgtcgccg gggcccatct gacccgtatc gaaataggcg
     6061 ccgaggggac actgataatt ggcacgcagg ttatcggcac tgttggagaa acagatcatt
     6121 ttctttgtcg ccgcactggg aaactggaaa ccgaatacca ggtatttacc ttgctgatcg
     6181 gcggggataa agaatccttt ggtcctttta tcgacccgcc cgatgatctg ggcggagagc
     6241 gcggtcgtga acaggaataa cgagatgaaa agcagtattt tatacatttt gtacagagga
     6301 atggttctcc tacatgctgc aaggaatata ccaaaatcta ccctccgacg cgtttccaga
     6361 agatgatcgt ccttcctgtt cggccgtgtc cagggtcagc agggtcctgc ccttttcaac
     6421 ggctatccgt tctgcctcgc gtaacaatgc gctggctact ccgcggcccc gatgggcgac
     6481 gctcgtcatc attttagcga tctccgcgcg atgcggctga ttctcgttac cgattgcata
     6541 aatccgatgg acccgtggtt cgcgacggtt tcgatcagca gatcggcaag cgcggcaatg
     6601 atctccggtt gcgcggtcag cgtgacgatg gtggttcggt tagacatgcg tcgatccttt
     6661 ttttctgtct gaaatgatgg cgcatatgca tttccgcgaa ttgcagccat tccagcgagt
     6721 tgaaataatg caacccgggg tgccgtgtct taccggtcgc cggcccctgg atcatcctgt
     6781 cgggaaatac atccttcgca ggcgcggaga atacggtgtc gatagtaact gtgttcatac
     6841 gtattcccgg ttgtaccggt tgcggtattg taccggcgag agccccgtca cttttttgaa
     6901 gatcatgcgg aaggccttcg gatcggaata acctactttg tccatggcct cgttgacacc
     6961 cagccggctg gattccaggt ttttcttgac cgcctccatc ttgacgcgct gcacatattc
     7021 catcaccgta ttgcccgtcg cttttttgaa acgtctttcc agatttctgc ggctgagcgc
     7081 cgccagcgac gccagcgcgt ccacgcttac ccgcgaatgg acattccgct cgatgaattc
     7141 ctgggctttt ttgatggcca ggtctgaatg gtccttctgc ccgctgaaaa taataaaggg
     7201 ggattggctg ctgcgttgca gatcgatctg gaaccccttg gcgcaggaga ccgcaatgtc
     7261 gcggccggca tatttctcga cgaggtacag cacgaggttc aaccaggaat atgcgcctcc
     7321 actgtataga tattgtcatt atcggtgatg atcttttctt ccagcaaatg gacggccgga
     7381 tacaatttcc ggaaatcatc ggccatttcc cagtgggtgg tacaactctt cccgtcgagg
     7441 agaccggtac cggccagcag gaaggcgccg atacagagcg aggctatcgc ggccccgccc
     7501 ttatattgtt cgacgatcca ggaaaggagc gcgtgattgt cggcgacgat cttttgtttg
     7561 tcgccatgga cggatgggat gatgatgagc gccgtccggg ccacctcccc gatgagctga
     7621 tcgggacaga ccatgaacat cccgttcttc atgttcacct gtttgagatg acctacgaga
     7681 tggacgtcga acagggcatc gcgcccggca tcttcgagat agccgttcac gcgcatcaat
     7741 atctgttggg tcccttcgat attcgtcagg ctgcaattgc cttccggtac gaggatagat
     7801 acgtgtatca tgctataaat gtaagcatac gatccgacta ttcgatcagg tggggcagga
     7861 agcggcttag gttgtcggtg atcagcgtac cggcctcgcg gataccgatg ccggcgggtt
     7921 gctggccgat gacccagctg ccgatcaggg ggtaattgtc cccgaaggca ggcaagggga
     7981 agaattcctg gcagatataa ccttcttccc cgtagtctcc atccgtccgg ccgacgagat
     8041 cctttccgac catgatggcg atattggccc cttcccggga aagaatcggc tttttgacat
     8101 agttggtcag cggctcgctg gaaaaatagg cgggcagcaa atgtggatgc ccggggaaca
     8161 gctcccaaag aagcggcaga atcgctttat tggaaaggat catcttccag gcgggctcga
     8221 tgaaaaacgc ctggttctta tcccgcccga tatgctcccc gaattcgtcc gccatcagcc
     8281 actcccaggg gtatagcttg aagagatttt ttatcgcgat ctgctggtcg tcgacgaaga
     8341 cctgctgttt ctcgtcccag ccgatgtctt cgacaaaaag aaaatgggtg gggatcccag
     8401 cctgtatagc gcagtcgcgc aggtattcca cattcgtgag gtcctccaga gtgtctttca
     8461 gacaggcgaa atggagggtt ccgggataga gatagggctg cagcccggtc cagtgttcga
     8521 tcagtttttc atggatgctg ttgaactggt ctttggcagg atggaaatcc tttagccaga
     8581 accactggac gatgcccgcc tcgaagagac tcgtgggggt atcggcgttg aactccagca
     8641 tttttacttt cccgtcgcga taacacagat ccatacgacc atagatggag ggatgatcct
     8701 ctctccagct ttcttccagg cagggcacga aagaccgcgg aatatggaac agatcgtaca
     8761 accggtgatc gatggtatat tggacggcct gcaaacaaag ctcccataat tgtgtcgtcg
     8821 cttcttcgat gagcaggatc tcgtccgtgc tgaaggaata ataggcgctt tcatcccagt
     8881 aaggcacatt ggtcgtatga aaaccgaaac ccaattgctc caccgttgag cgccagttgt
     8941 tgcggggtgg tatcgcgatg cgttgcatgg attggggatt ttacaggatc aggaggcggc
     9001 ggatgagccg gcggaagctg ctgatgagcc ggcggaagct gctgatgagg cggcggaagc
     9061 tgctgatgag ccggcggaag cggccttgcc gaacccaccg cggacgacga aggtatgatt
     9121 ccggaagctg gtattgatgg gataacgcgg cgcggcccgg taagggacgt tgaatggccg
     9181 accggtatag aagatattga agttgaagaa cgggttgtta taataatcgt agggattgta
     9241 cccgttgtag ttattggaga cgggctgaca cccgcagtcg tagacactgt cttcttcggc
     9301 cgtggctgca gtgagagcag aatctgtgcc ggagacttcc gccgattgac cgggaatgat
     9361 caccctgttg caggaggcca gcgcggcggt gatgagcacg agatgaatgg ttttggtctt
     9421 cttcatagtt tttaatttga tccgaaaaga aggtcgaggg taaaagcgtt gccgctgaga
     9481 tcggtgcctc cgtcgttgtt ataatgaatg atatgatatt ttgcggtcaa tcccagaaag
     9541 gcgtggcagt gcaggaaata cttgaaacga agcccatagt tcagatcgaa ggagcctacg
     9601 ttcagcgggg tgatatttac ggcccggtgg tgatcgctcc agccgtcggc cacggaaaaa
     9661 tagtcgtagg cgatgccggc ggtgtagccg agatcgacat gttttttatg aacgaaatac
     9721 cggctgtatt cgaagccgat gtagccgccg tcataatagt tgctggtgta gacagtatcc
     9781 tgccggagga aggtgtaggg acgcggtgtg ggataaagga aacggacgtt ccaggtgata
     9841 tcgtattcat tgcgtggggt gcgatagccc agctgcactc ctacagaagg atgcgagccc
     9901 agcacctgtg agaggtggtc ggtcggaaac caccaaccca tcatgacggc ggccgtgccc
     9961 gtgtgcgcat cgcggcagtt catgaatcca agatcgatgt atttttgaag gttcttttcc
    10021 gcctgggcaa tccgtgggca tgattcggga aaggctttga gcaaagcctt cggatcggcg
    10081 atatccccgc tgaccgttcg gcagatgaag gagcctgtgg tgtcgagggg atgatctttt
    10141 cggatctcct gtgcccagga
//
LOCUS       DASYSV010000332         5157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_836165, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000332 DASYSV010000000
VERSION     DASYSV010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836165"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /gene="asnB"
                     /locus_tag="VGQ51_09975"
     CDS             complement(<1..889)
                     /gene="asnB"
                     /locus_tag="VGQ51_09975"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV9036940.1"
                     /translation="MCGIAGILSADTAQITQGRLEQMTSSLAHRGPDGGGIWISGSGM
                     AGLGHRRLSIIDLSPAGDQPMHYLGRYTIIHNGEIYNYPELRSLLRTKGYTFTSQTDT
                     EVILAAYAHFGADCLQYFDGMFAFAIWDSTEDRLFLARDRFGEKPLFFYRDSERLLFA
                     SELKALWAAGIKKEINPRMLFNFLTIGYTQNPGDGFETFFRDISKLPPRSWLYYYPST
                     GRMETATWWDIEPGQTKTIKEESAVDTFCDLLYTSVKRRLRSDVRVGTSLSGGLDSSS
                     VVACILRQKEAPEGLPTFSA"
     gene            complement(917..1480)
                     /locus_tag="VGQ51_09980"
     CDS             complement(917..1480)
                     /locus_tag="VGQ51_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEV9036941.1"
                     /translation="MKSIVLIAGLVMGMTLAHAQSSTSNLDSSPLDMSYYPVDYPVLK
                     IQDKVSEPLVARVVYSRPQKKGRRLFGDLVAYGQVWRLGANEATEIEFFRDVKIDNKT
                     VKKGRYTLYALVNEDKWTLIFNKETDTWGAFRYDSSKDVLRTTVPVEKQAVITEAFSI
                     AFRKAEKGADLVIDWDDVQVSLPIAWK"
     gene            complement(1510..2109)
                     /locus_tag="VGQ51_09985"
     CDS             complement(1510..2109)
                     /locus_tag="VGQ51_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HEV9036942.1"
                     /translation="MNYLAHAYLSFNIPEITVGNLISDFVKGRKKLDYPVAIRRGIDL
                     HRAIDTFTDNHEVTRRAKSFFKDAYGLYSGPLTDIVYDHFLANDPGIFPLMDGGLKVF
                     SSTVYRHIETCREALPENFAHLFYYMRTQDWLSHYGEKQAIRNSFGGLARRATYMPDP
                     RRAGELFEIHYLELEACYRDFFPGLEEFARHTLREGQMH"
     gene            2452..2907
                     /locus_tag="VGQ51_09990"
     CDS             2452..2907
                     /locus_tag="VGQ51_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036943.1"
                     /translation="MKKIILSIATVLMISAGAFANGNASFIGDNVNQQAIASFKKDFA
                     AASNIMWEQKVGYSKATFSLNGEILFAYYDNNGELTAVVRNITSEQLPINLITSMKRD
                     YDGYWISDLFEVASGDQTTYYVTLENSDKKIVLRSFGIDSWNVYSKEKK"
     gene            3002..3976
                     /locus_tag="VGQ51_09995"
     CDS             3002..3976
                     /locus_tag="VGQ51_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEV9036944.1"
                     /translation="MSKGPVSQFIEHHYRHFNAAALMDAAKGYETHLTEGGKMMITLA
                     GAMSTAELGISLAEMIRRGKVDIISCTGANLEEDVMNLVAHSHYKRVPNYRDLTPQDE
                     WQLLENHYNRVTDTCIPEEEAFRRLQKHLVKVWKDAEAKGERYFPHEFLHQVVLSGEL
                     EQYYEIDPKDSWIIAAAQKNIPIVVPGWEDSTTGNIFASYVIKKELKAGTVKSGIEYM
                     TWLADWYKANSGGKGVGFFQIGGGIAGDFPICVVPMMYQDLGWHDVPFWGYFCQISDS
                     TTSFGSYSGAVPNEKITWGKLDIHTPKYIVESDATIVAPLIFAWVLGW"
     gene            3992..4507
                     /locus_tag="VGQ51_10000"
     CDS             3992..4507
                     /locus_tag="VGQ51_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036945.1"
                     /translation="MTTNSEELYLEAEADIKNNNYAEAFKKYESILYEEPDSAPAHNS
                     MGWLYKTQFDDYGKAENHFQTAIRCDPQYPHSYFHIAALLMDMERFEELRNHLDRCLR
                     VRTIDKSWVYGRYALSEELQKNYEKAVYYFEKAILASQNDEKIKDYQHDIERCRMKQE
                     ILGKHNEEQKK"
     gene            complement(4754..>5157)
                     /locus_tag="VGQ51_10005"
     CDS             complement(4754..>5157)
                     /locus_tag="VGQ51_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEV9036946.1"
                     /translation="SATDLINKFSRERKSIAWVVDEFGGTAGIVTMEDLLEEIFGDIR
                     DEYDTEEFEERKLSNDEFIFSGRLELDYLNRKYGLDLPENDSETLSGYIINQHETIPH
                     LKDRIILGDYEFEILNVSDTRIEMVRLKVLK"
BASE COUNT         1317 a         1290 c         1339 g         1211 t
ORIGIN      
        1 tagcggaaaa ggtgggcaag ccttccgggg cctccttctg gcgcagtata caggccacca
       61 ccgacgagct gtccaggcca ccgcttagac tggtccctac gcgcacgtcg ctgcgcagtc
      121 ttcgtttcac cgaagtatac aacagatcgc aaaaggtgtc taccgcgctt tcctctttga
      181 tcgtcttcgt ttgtcctggt tcgatgtccc accaggtggc agtttccatt ctgccggtgg
      241 aaggataata gtatagccag gagcgtggcg ggagtttcga gatgtccctg aaaaaagttt
      301 cgaaaccatc cccgggattc tgggtgtagc cgatggtgag gaaattgaac agcatccggg
      361 ggttgatctc cttttttatt ccggccgccc ataatgcctt gagctcgctg gcaaaaagca
      421 ggcgttcgct gtcgcggtag aagaacaggg gtttctcgcc aaaccggtcg cgggccagga
      481 atagccgatc ctccgtcgaa tcccagatgg caaaggcgaa catgccgtcg aaatattgaa
      541 gacagtccgc cccgaaatga gcataggcgg ccaagatgac ctccgtgtct gtttgagagg
      601 tgaaggtata acccttcgtc cgaagcaggc ttcgcagttc cggataattg taaatctcgc
      661 cgttgtggat gatggtatat cggcccaggt agtgcatcgg ctggtcgccc gcgggactca
      721 gatcgatgat ggacagacgg cggtgcccca acccggccat gccggacccg ctgatccaga
      781 tgcccccgcc atccggaccg cggtgggcca gcgaggacgt catctgctcc agccgccctt
      841 gagttatctg cgctgtgtcg gcggaaagaa taccggctat accgcacata tgaatttatt
      901 tatggtgtgg ccaggctcat ttccaggcta tcggaaggct tacctgtacg tcgtcccagt
      961 cgattacgag atcggcgccc ttctccgcct tccggaaggc gatggaaaaa gcttcggtga
     1021 taactgcctg tttttcgacc ggaaccgtcg ttcgcaggac atctttggag gaatcgtaac
     1081 ggaaggcgcc ccaggtatcc gtctccttgt tgaagatcag tgtccatttg tcttcattga
     1141 cgagcgcata gagggtatag cggccctttt tgacggtctt gttatcgatc tttacatccc
     1201 ggaaaaactc gatctccgtt gcctcattgg ccccaagccg ccatacctgg ccatatgcga
     1261 cgaggtctcc gaaaagtctc cgcccctttt tctgaggccg gctataaacg acgcgggcga
     1321 ccagcggttc gctcacctta tcctgtattt tcagcaccgg gtaatccacc gggtaatagc
     1381 tcatatcgag aggactgctg tcaagattgg atgttgaaga ctgtgcatgg gccagggtca
     1441 ttcccatcac taaaccggcg atcaaaacaa tagacttcat aacgcaacaa cggtttgaat
     1501 agtgtaaaat tagtgcattt gcccttcccg gagggtatgc cgcgcaaatt cctccaggcc
     1561 ggggaagaaa tcccggtaac aagcctctaa ttcaagataa tgtatctcga agagttctcc
     1621 tgcccggcgc gggtcgggca tataggtggc tcgccgggca agaccgccga aactgttccg
     1681 gatggcctgt ttttcgccgt aatgcgatag ccagtcctgg gttcgcatat aatagaacag
     1741 atgtgcaaaa ttttcgggaa gggcctcacg gcaggtctct atgtggcggt agaccgtcga
     1801 cgaaaatact ttcagccctc catccatcaa cggaaaaatt ccgggatcat tggctaaaaa
     1861 atgatcataa acgatatctg tcaacggtcc cgaatacaac ccatacgcat ctttgaaaaa
     1921 tgatttggcc cggcgggtga cttcatgatt gtcggtgaac gtgtcgatcg cgcggtgaag
     1981 atcgatgccc cggcggatgg caacgggata atcgagtttt tttcttcctt tcacaaaatc
     2041 actgatcagg ttgccgacgg ttatttcggg gatgttgaaa gaaagatagg cgtgagcgag
     2101 atagttcatc cttacaattt aaatgttttt ttgccgaaaa attaatttaa caccgtctgg
     2161 tgtgatttta aactttcatt aagaaatcgg ctctaaaaca gtcgtggcgc gggtttgagg
     2221 cgttcccatc ctgggagggg cattaccgtg gaacatcgtt gttcatgatt ccgtaatatc
     2281 aacctccgtt ggtcactatt ggcttgcaag cacgcgtaac cttggatagt tttgctgcgt
     2341 cttagttaat gaaaatattt tttgacgacg ctgcaacatc ggatcaaaag ccagcgtcta
     2401 ctaatacaga gctcaccaaa cacacaattt aaaaacttac aaaaatagaa aatgaaaaag
     2461 atcatcttat ccatcgctac cgtgcttatg atcagtgccg gcgccttcgc caatggcaat
     2521 gcaagcttca ttggagataa cgtcaatcaa caggcgatcg cctcgttcaa aaaagatttt
     2581 gccgccgcga gcaatatcat gtgggaacaa aaggtgggct attcgaaagc cactttctct
     2641 ctcaacggcg agatcctgtt cgcttattat gacaataacg gcgagctcac tgccgtcgtc
     2701 aggaatatta cttccgaaca gttgccgatc aacctgatca cttcgatgaa acgggattat
     2761 gacggttact ggatcagcga tctgttcgaa gtggcttcag gggatcagac gacttattat
     2821 gtgacgctcg agaacagcga taagaagatc gtattgcgct ccttcgggat cgactcctgg
     2881 aatgtttaca gcaaagagaa aaaataggac aagcttagca ggttagtttt tagttggaaa
     2941 atcgctccga acaaataaag taggggcgat ttttgtattt ttgcggctca atcgcaaaac
     3001 catgagcaag gggcctgtct cgcaattcat tgagcaccac tatcgtcact tcaacgcagc
     3061 cgcattgatg gatgcggcga aaggatatga aacacattta acggaaggcg gaaagatgat
     3121 gatcacgctc gctggcgcca tgagcacagc ggaactcggc atatcactgg ccgaaatgat
     3181 ccgccgggga aaggtggaca tcatcagctg caccggcgcc aatctggaag aagacgtaat
     3241 gaacctggtc gcccatagtc actacaaacg ggtaccgaat taccgcgatc ttacgccgca
     3301 ggacgagtgg cagctcctgg aaaaccacta taacagagta acggacacct gtatacccga
     3361 agaggaagcc ttccgccgtc tccaaaaaca ccttgtgaag gtgtggaagg acgcggaagc
     3421 aaagggagaa cgctatttcc cgcatgaatt ccttcaccag gtggtcctca gcggcgagct
     3481 cgaacagtac tatgaaatag acccgaagga tagctggatc atcgccgcgg cgcagaaaaa
     3541 tatcccgatt gtcgtacccg gctgggaaga cagcaccacg ggcaacattt ttgcaagcta
     3601 tgtcattaag aaagagctca aagcgggcac cgtgaagagc ggtatcgaat atatgacgtg
     3661 gctggcggat tggtataaag ccaatagcgg cggcaaagga gtaggctttt tccagatcgg
     3721 aggcggcatc gcgggagatt tccccatttg cgtggtgccc atgatgtacc aggacctcgg
     3781 atggcacgat gttccgttct ggggctattt ctgccagatc tcggattcca ccacctcctt
     3841 cggttcttat tccggcgcgg tgccgaatga gaagatcacc tggggcaagc tggatatcca
     3901 cacgccgaaa tatattgttg aaagcgatgc caccatcgtc gccccgctga tcttcgcatg
     3961 ggtgcttggg tggtaacgat caaaatctgt tatgacaaca aattcagaag agctgtacct
     4021 ggaagccgag gccgatatca agaacaacaa ttacgccgaa gctttcaaga aatatgagag
     4081 catcctttat gaggagccgg atagcgcgcc cgcgcataat tcaatgggtt ggctgtacaa
     4141 gacccagttc gatgattatg gcaaggcgga gaaccatttc caaaccgcca tccggtgcga
     4201 tccccaatat ccgcattcat attttcatat cgcagcgttg ctgatggata tggaacgttt
     4261 cgaagaactg cgcaatcacc tcgaccgttg tcttcgtgtc cgcaccatcg acaagtcctg
     4321 ggtctatggc cggtatgctc tatcggaaga gttgcagaaa aactacgaaa aggcagtcta
     4381 ttatttcgag aaggccattt tagccagcca gaacgacgag aagatcaagg actatcagca
     4441 tgatatcgag cgttgccgga tgaagcagga gatcctgggg aaacacaacg aagagcagaa
     4501 gaaatagggc gatcgcgccg gcgatacaga agcgatccgc gggacctgta taaaaaaaac
     4561 aagcactcca ttacagagtg cattgtttac tcctaactga cctatgaaaa aagttgagtt
     4621 gtttcgcttt tgcagaacga agatgatcaa aaaccggagg atggaaaaat aacgggggct
     4681 gattaaccac gggttaacat tggttaacaa tggggtttgg cgtgcccggc gggccccggc
     4741 cggaagactt cgcctatttt agcaccttca atcgtaccat ttctatacgc gtatcgctga
     4801 cgttcaggat ctcgaactca taatctccca gaatgatgcg gtctttcaga tgtggaatgg
     4861 tctcgtgctg gttgatgata tagcccgaga gcgtttccga gtcgttttcc ggaaggtcta
     4921 gcccatattt ccggttcaga tagtccagct ccagccggcc ggagaagatg aattcgtcat
     4981 tggaaagctt ccgctcctcg aactcctcgg tgtcatattc gtcacggatg tctccgaata
     5041 tctcctccag cagatcttcc atcgtcacga tgcccgcggt gcccccgaat tcatcgacga
     5101 cccaggcgat actttttcgc tcgcgactga atttattgat cagatcggtg gcgctca
//
LOCUS       DASYSV010000333         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1986264, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000333 DASYSV010000000
VERSION     DASYSV010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986264"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGQ51_10010"
     CDS             complement(<1..434)
                     /locus_tag="VGQ51_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9036947.1"
                     /translation="MTINTNSNPIGTNSIPAGTTPGPIKAIIIDDEPLARSIVSEYLQ
                     AWPQIDVVQECNDGFEGIKAIQHHHPDLLFLDIQMPKINGFEMLELVEQPPAVIFTTA
                     FDEYAIKAFESHAVDYLLKPFNKDRFDKAIQKWLDQRAPNPPS"
     gene            complement(431..1480)
                     /locus_tag="VGQ51_10015"
     CDS             complement(431..1480)
                     /locus_tag="VGQ51_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9036948.1"
                     /translation="MARSPLSTGKLLLIFLSWWAIWAFLQISLLIGFGLSSGTAVTDS
                     LVSNSLVAISCAFLSNNMQYYLPKKERYWYILFISLGLSGIILLACKAILVPLLDDKT
                     ESVNYVIFFSRSWAVRFDIVFLQVACMSILSVLWYSSEEEQENRQRRNDAERLAKEAE
                     LFKLRQQLAPHFLFNSLNSISALIASRPEQARKMIQQLSDFLRGNLRKEEQQWVPLSE
                     ELQYLELYLDIEKVRFGHRLSTDIRCEDECLGMRLPPMLLQPIVENAIKFGLYDTTEA
                     VTIAIRARLDGNYVRIEVENPFDPETSRPKKGTGFGLTSIQRRLYLLFARPDLLETRV
                     DNNLFTTIVRIPQQI"
     gene            complement(1482..1841)
                     /locus_tag="VGQ51_10020"
     CDS             complement(1482..1841)
                     /locus_tag="VGQ51_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HEV9036949.1"
                     /translation="MNWYLSKIIFRIICGEGHHTPQFDEQLRLISAHDAEEAFEKAMS
                     IGHREQDRFYNHEQQLVQWKFVNVAELYLLSGLLDGAEMYSRIQETDDAEAYIDHANH
                     LAEHIRTSSTHKILQLL"
     gene            complement(1878..2837)
                     /locus_tag="VGQ51_10025"
     CDS             complement(1878..2837)
                     /locus_tag="VGQ51_10025"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEV9036950.1"
                     /translation="MIDDDRQVRRRCGNGRIWAGLFLLLVGGVLLLDQMGFPLPDWLF
                     SWHVLLVAIGIFIGLRHNFRGGAWFILIAIGGVYLIQDYNPDINLHRFIWPGILIIIG
                     LVFVLRPRQRWEADDEWKYKMKREIKDKWARRQMKHEWRHHGWNRYSQPPNDPNNPNN
                     PGNPNNPNNPNNPNNPNDPNNPINPGDPNNTSGSGGTYGSYSGPSWSSEDYIDTTSVF
                     GGVHKKVVSKNFKGGDVTTFLGGTEIDLTQADINGVVRLDVTQVMGGTKIIVPAHWEV
                     RSQVTAVFAGFEDKRQQPAGLNPDKILIIEGTSIFGGIELKNY"
     gene            3041..>4700
                     /locus_tag="VGQ51_10030"
     CDS             3041..>4700
                     /locus_tag="VGQ51_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9036951.1"
                     /translation="MIKNWFRTALRNFRKNGMATAINIFGLTIGLSSCLLIALFIRHE
                     LSYDDFEVNGPRIARVIMEYRFDGSPDSRRGNFTSTKVAPTFRRVFPEVESAVRMTEN
                     DRIVGYGEKLFYETKFMYADSSFFDVFSFSLLRGDRRTALSGPKKVILTESTARKYFG
                     DSDPMGKLLRIGTDSVDYQVTGIMPDVASNSQFKFDFLASFSSLYANQEETYWDANYG
                     TFLLLHHPGDIAALQPKVDAFMKQEMKGQGATIKFFLEPFMRIHLYSEYIGFEPGTSI
                     VYIYILAGVALLILIIACSTYINLSTARSVDRAREVGVRKVVGAGRGQLFWQFIGESF
                     LLCMVAVVLSLVVVLLVLPGFNRLADRRLPAASLFSPLFLLMALGIAVVVGLLAGGYP
                     ALVLSRFHPVKVLKGAFRNTRSGQGLRQSLIVFQFVISVFLIVATGIMQRQLSYIQNR
                     KLGYDRDHVIELPIVSQMLPGIDYFKQQLKTNPDVLSVTHCSSSPVVIQGGYNMRSAA
                     MQQGVQMNVYGNPIDEDFIPTTGLQLVAGENITAQDMRDADPSDF"
BASE COUNT          978 a         1062 c         1502 g         1158 t
ORIGIN      
        1 gagggagggt ttggggcgcg ctggtcgagc catttttgaa tggccttgtc gaaacgatct
       61 ttattaaagg gtttgaggag atagtccacg gcatgacttt cgaaggcctt gattgcgtat
      121 tcgtcaaagg ctgtcgtgaa gatgacggcc ggcggctgtt cgacgagctc cagcatctcg
      181 aacccgttga tcttgggcat ctggatgtcg aggaagagga gatcgggatg gtgatgctgg
      241 atggctttga tcccttcgaa gccgtcattg cattcctgca cgacgtcgat ctgcggccag
      301 gcctgaaggt attcactgac gatcgagcgc gcgaggggtt cgtcatcgat aatgatggct
      361 ttgatcgggc caggggtggt gccggcgggg atgctgttgg tgccgatggg attgctgttg
      421 gtgttaatgg tcatatttgt tgtgggattc ttacgatggt ggtgaaaagg ttgttatcga
      481 cgcgggtctc gagcaaatcg ggccgggcga acaacagata aagacgacgc tggatcgaag
      541 tgagtccgaa accggtgcct tttttgggcc gcgacgtttc gggatcgaag gggttttcga
      601 cctctatacg cacatagttg ccgtcgagac gcgcgcggat ggcgatggtc acggcttcgg
      661 tggtatcata caatccaaat tttatggcgt tctctactat tggttgcagc aacatgggcg
      721 ggagccgcat gcccagacat tcatcttcac aacggatgtc ggtggacagg cggtggccga
      781 agcgcacctt ttcaatatcg agatagagtt cgagatactg caattcttcg gagaggggca
      841 cccattgctg ctcttctttc cggagattgc cccggagaaa atcggagagc tgctgtatca
      901 ttttgcgggc ctgctccgga cggctggcga tcagcgcgct gatagagttg aggctgttga
      961 agagaaaatg cggtgcgagc tgctgacgca atttgaacag ttcggcttct tttgcgaggc
     1021 gttcggcgtc gttgcgccgc tgccggttct cctgttcttc ttcactggaa taccagagca
     1081 cgctgaggat ggacatacag gccacctgga gaaagacgat gtcgaaacga acggcccagc
     1141 tacggctgaa gaagatgaca taattgacac tctcggtctt atcgtcgagc aggggaacca
     1201 agatggcctt gcaggccagc aggatgatcc cgctgaggcc aaggctgata aaaaggatat
     1261 accagtaccg ttcttttttt ggaagatagt actgcatgtt gttgctgagg aaggcgcagc
     1321 tgatggcgac gaggctattg gatacgagac tgtcggtgac ggcggtgccc gaggagaggc
     1381 caaacccgat gagcaacgat atctgcagga aggcccagat ggcccaccag gagaggaaga
     1441 tcagaaggag tttgccggtg gatagcgggg atcgcgccat attacagtag ttgcaggatc
     1501 ttatgggtac tgctggtgcg gatatgttcc gccaggtgat tggcatgatc gatatacgcc
     1561 tcggcgtcat cggtctcctg gatccgcgaa tacatctccg caccgtcgag cagtccgctg
     1621 agaagataga gttccgcgac attgacgaat ttccactgga ccagctgctg ttcgtggttg
     1681 tagaacctat cctgttcacg atggccgatg gacatggctt tttcgaaggc ctcttcggca
     1741 tcatgcgcgg agatgagacg cagctgttca tcgaattgcg gagtgtggtg tccttcgccg
     1801 cagatgatcc ggaaaatgat cttgctgaga taccagttca taagacttag ttttcgcgcg
     1861 cccttgcggg cgtgcggtta gtagtttttt agttcgatcc caccgaagat ggacgtccct
     1921 tcgatgatga gtatcttgtc gggattgaga ccggcaggct gctgtcgttt gtcctcgaaa
     1981 ccggcgaaga cggcagtcac ctgtgagcgg acttcccaat gcgccgggac gatgatcttg
     2041 gtgccgccca tcacctgtgt aacatccagt ctcacgaccc cgttgatatc ggcctgcgtg
     2101 agatcgatct cggtgccgcc gaggaacgtg gtgacatcgc cgcctttgaa attcttcgag
     2161 actacctttt tatggacgcc gccgaagacg gaggtcgtgt cgatataatc ttccgaggac
     2221 caggagggtc cggagtatga gccgtaggtg ccgccggaac cggaagtgtt gttgggatcg
     2281 ccggggttga taggattgtt agggtcgtta gggttgttcg ggttgttcgg gttgttgggg
     2341 ttgttggggt tgcccgggtt attggggttg ttgggatcgt tcgggggctg ggaatagcgg
     2401 ttccagccgt gatggcgcca ttcgtgtttc atctggcggc gggcccattt gtcctttatt
     2461 tcgcgtttca ttttatactt ccattcatcg tctgcttccc aacgttggcg aggccggagg
     2521 acaaagacca gaccgatgat aatgagaata ccgggccata taaaacgatg aaggttgata
     2581 tcggggttgt agtcctggat caggtagact ccgccgatgg cgatgagtat gaaccatgcg
     2641 ccgccgcgga aattgtgtct gaggccgatg aagatgccta tcgccacgag caggacgtgc
     2701 cagctgaaaa gccaatcggg aagaggaaag cccatctgat ccagaaggag tactccacct
     2761 accagcagca ggaaaagacc tgcccagatc cggccgttcc cacatctcct tcgaacttgt
     2821 ctgtcgtcat caatcattat aatcgaattt tagtcaaatc tacggggtat cgggggggcg
     2881 gacaagtgtt ggtaggccgt aaaggtctcc ggggcggtgg atggcggggt tttgtcggtg
     2941 aaaccgctgg agtgatgagc ggtttggggg agggggttgg tggaaggacg gggtggatgg
     3001 gcggcagagg gtttgtacgg gggctttcgt agatttgggt atgatcaaaa actggttccg
     3061 cactgcgttg cgcaatttcc gaaagaatgg gatggctacg gccatcaata ttttcgggct
     3121 gacgatcggg ttatcgagct gtctgctcat cgcccttttt atccgtcatg agttgagcta
     3181 tgatgatttc gaggtcaatg ggccgcggat agcgagggtg atcatggaat accgttttga
     3241 cgggagtccg gactcgcggc ggggtaattt cacgagtacg aaggtggcgc cgacgttcag
     3301 acgggtattc ccggaagtgg agtcggcggt gcgcatgacc gaaaatgacc ggatagtagg
     3361 atatggcgaa aagcttttct atgaaacgaa attcatgtat gcggacagca gctttttcga
     3421 tgtcttcagt ttttcgctgc tgcggggcga caggcggacc gctctgtcgg ggccgaaaaa
     3481 agtgatcctc accgagtcta cggcacggaa atattttggc gacagcgatc cgatggggaa
     3541 gctgctccgt atcggcaccg acagcgtcga ctaccaggtg acggggatca tgccggatgt
     3601 ggcttcgaac tcgcagttca aattcgattt cctggcttct ttttcctcgc tgtatgccaa
     3661 ccaggaagag acctattggg atgcgaatta tggcacgttc ctgctgctgc atcatccggg
     3721 ggatattgca gcgctgcaac ccaaggtcga cgcattcatg aagcaggaga tgaagggaca
     3781 gggggcgacg atcaaattct ttctggagcc gtttatgcgg attcatcttt attcggaata
     3841 tatcggtttt gaacccggaa cgagtatcgt ctacatttat atactggcgg gggtggcgct
     3901 gctgatcctc atcattgcct gttccactta tatcaatctg agtaccgcgc ggtcggtaga
     3961 ccgggcacgg gaggtagggg tcaggaaggt ggtgggcgcg ggacgcggac agctgttctg
     4021 gcaatttatc ggggagtcat tcctgctgtg tatggtggcg gtggtattga gcctggtagt
     4081 ggtgttgctg gtgttgccgg gttttaaccg gcttgcggac cggcggctgc cggcggcgtc
     4141 acttttttcg ccgttgtttt tattgatggc attggggatc gcggtggtgg ttggtctttt
     4201 agcgggcggc tacccggcgc tggtattgtc acggtttcat cccgtgaagg tgctgaaggg
     4261 agcatttcgg aatacgcgca gcgggcaggg gttgcggcag tcattgatcg tatttcaatt
     4321 cgtcatttcg gtgtttttga tcgtggcgac cgggatcatg cagcggcagc tgagctatat
     4381 ccagaaccgc aagctgggtt atgaccggga tcatgtcatc gagctgccga tcgtcagcca
     4441 gatgttgccg gggatcgatt atttcaaaca acagttaaag accaacccgg atgtgttgag
     4501 cgtaacacat tgctccagtt cgcccgtggt cattcaggga ggttataaca tgcggagcgc
     4561 ggcgatgcag caaggcgtac agatgaacgt atatgggaac cccatcgatg aagatttcat
     4621 acctactacc ggtcttcagc tggtggccgg cgaaaatata acggcgcagg acatgcgaga
     4681 tgcagacccg tcggatttta
//
LOCUS       DASYSV010000334         9504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1473283, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000334 DASYSV010000000
VERSION     DASYSV010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473283"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VGQ51_10035"
     CDS             <1..707
                     /locus_tag="VGQ51_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEV9036952.1"
                     /translation="IEGAYASFAGSPTSRGEFQFDLWGVEPTLRWDWYRLKSEVVKHG
                     VRNSLLVAPMPTASTSQILGNNECFEPYTSNIYVRRVLSGEFVVVNKHLLKDLVELNL
                     WNDDMKNKIILCNGSIQTIDGIPQEIKDIYKTVWEIKQRTIIDMAADRGAYICQSQSL
                     NLFVDAPSSNKLTSMHFYAWKKGLKTGMYYLRTQAASQAVQFTVERQGGKMVEPMEPP
                     AGATCSMQEGCVTCSA"
     gene            complement(903..2630)
                     /locus_tag="VGQ51_10040"
     CDS             complement(903..2630)
                     /locus_tag="VGQ51_10040"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV9036953.1"
                     /translation="MDAAILDKVNKWLNGNYDQATKDEIQRLQKENPNELTESFYRNL
                     EFGTGGLRGIIGVGTNRMNKYTVGMATQGFANYLKASFPGKEVRVAIAHDSRNNSRFF
                     AETVANVFAANGIKVFLFQSLRPTPELSFAIRHLHCQGGVVCTASHNPKEYNGYKAYW
                     DDGGQLVPPHDKNVIQEVEKIAGVDAVKWGGGESNISMIGAAMDEAYIGMVRNLSVYP
                     EVIERQHNLRIVYTPIHGTGIKLVPEVLQRFGFSNVHVVDEQATPDGNFPTVIYPNPE
                     ESEAMSIGLRKAQQIDADILLGTDPDADRVGIGVKDKKGQWVLMNGNQTATLAFNYMI
                     EARKAKGVAAANDMVVKTIVTTNLIDEIAWRNNISCYNVLTGFKYIAELIKEKEGREN
                     YVIGGEESYGLMIGSQIRDKDAVSAVALLCEMAAYEKDKGRSLYDKLIDLYVEYGFYK
                     ESLISITKKGMNGQKEIAAMMSAYRSQPPAAINGSPVVELLDYELRKGKNLQTGESWD
                     LELPESNVLQFILEDGSKISARPSGTEPKIKFYFSVHGPLADAAEYDKVNAELSDRID
                     RIIKDMQLK"
     gene            2823..4076
                     /locus_tag="VGQ51_10045"
     CDS             2823..4076
                     /locus_tag="VGQ51_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036954.1"
                     /translation="MYLLLRNNKQSGPYSLDDLKNMGLKAYDLVWLEGKSAAWRYPSE
                     IEELKEFAPAVEEQPFDRFYKKPAAAAVSASNGNQDTTTISFRPATEVSAPDDKSVTT
                     HKRIIYVTLPSGKPAPQPMREAPVREIERPRQLIPEPVTPVSATPATGDYPSLSDHRD
                     RTPVEFAPRHRSHQKERLFRVAGIGGSILALLAAGIFIGLSLNKESLGLQRRIASRTT
                     PQSVAKQTNPQPTVIPAANAAILANTTEKTTAPVGDNTISSTVSAVRRVRKQKEKIPA
                     ALMPPVQKDSVLADIPVVTREAVHRTQAEPSIDSKEAAKTAISSEVSVGANRYSVGTF
                     GGINDLQLTVSNRSAYPLDLVVVEVQYIQANKKVYKTENLSFRGISPGAALMQEAPKS
                     SRGIRVQYKITSISSKELGLSYSGL"
     gene            4294..4611
                     /locus_tag="VGQ51_10050"
     CDS             4294..4611
                     /locus_tag="VGQ51_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036955.1"
                     /translation="MRSAIVQLFLVLVGISGIHAVRLLQTSSFHVRLFPANGAGRVWA
                     IQGKDSIEMTNVNGEYILRSVKPGNWEVSVQANGPYRSACFQSGDLKPGMDRDLGEIR
                     LIR"
     gene            4688..5992
                     /locus_tag="VGQ51_10055"
     CDS             4688..5992
                     /locus_tag="VGQ51_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEV9036956.1"
                     /translation="MKRILLFGAGKSATSLIRYLIDVCVSRDWQLTVAETNLDLARSK
                     IGRVSQASAVQLNVTDENERDALVDGADVVISLLPPNLHYLVALSCLKKNKHLLTASY
                     LDSAIRTLEGDIRRKGLFFLGEMGLDPGIDHMSALQLVRRIKDDGGIIRTFHSHTGGL
                     IAPESDDNPWHYKISWNPRNVVLAGSAGATYREKGKVITRDQLSLFERPGTVSLEDFD
                     IGPLAWYPNRDSLSYIPLYDLEGTETFVRTTLRYPAFCRGWSAIVRAGLTDDKVPVDP
                     SGLSYAVWSAPLRALVGVDNREQLDFLGLFDKGPVPKTARTSADILQPLLETRLAMKP
                     ADRDMIVMIHEIGFTQAERAFMARSTLVVKGEDNLHTAMARTVGLPLGIAAVLVLEGR
                     ITLTGLHIPILPEIYEPVLEALGREGIAFGDTVKPVAPSPHA"
     gene            complement(6032..7384)
                     /locus_tag="VGQ51_10060"
     CDS             complement(6032..7384)
                     /locus_tag="VGQ51_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036957.1"
                     /translation="MAALRFMLLAGLLLSTLQGLSQEDELSASVARLRNYCRDSTPTG
                     YSPRMNVMTLTPNGNLHYLFPLYHALKDEEKFRKIYSDKGFYDEVSQYFAFAGDYRTA
                     LQYLVRSYDTIDDATRRKIYRTAAGLGNIVHADARRYIHLAAKDRQVVMINESFSKPL
                     HRAFTLSLLADFYRMGFRYLAMEMLDNFSNRRLTSVGMHSGYYVCEPVAGELMRTALA
                     MGFTLVPYEDTLAGAHTADQRDSVQAAHLFEVLRKDPKAKILVHASLTHIVKKALPDG
                     HIPMALAFWRMSGIEPLTIDQTDMTEESSFGYGRVIYQAYTTKFHLTEPSIALINDAP
                     VNVTDKDLYDLCVIHPPTTWLDGRPTWLSLDGLRQPTYIKAPSKEVFFVQAYYQKEID
                     ANDNTPWQLVPADQTYTPGGRTPTGIVRYLLYLRKGKYKIFFRDINYRILSALSIDVN
                     "
     gene            complement(7441..7953)
                     /locus_tag="VGQ51_10065"
     CDS             complement(7441..7953)
                     /locus_tag="VGQ51_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036958.1"
                     /translation="METTASETGGWLQLVLLGVFLIIAILFLLTEQNTLRTIKNENRR
                     IRPGQVWLQMIPIFGQIWQFIVVVRIAGSIRNEVASWENDSILGIEAAAIAQGNKRPT
                     QAIGIAYCTLIVIGVGLNPVLWGNPKVPIGLIELIGWTELAGIICWIVYWVKLAGYKR
                     RLRQKNLATL"
     gene            complement(7984..8676)
                     /locus_tag="VGQ51_10070"
     CDS             complement(7984..8676)
                     /locus_tag="VGQ51_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEV9036959.1"
                     /translation="MAKKKLVVLTGAGISADSGLKTFRDSDGLWEGYDVTEVATPRAW
                     RKDPALVLDFYNMRRRDTLKAAPNEGHTGLAQLEKDFDVHIITQNIDDLHERAGSTSV
                     MHLHGSIFQMRSEKDETLVYDIREDILLGHRAEDGAQLRPHIVWFEEPVPMIGYAVPL
                     VRTADIFVIVGTSLVVYPAAGLVDLAMPGIPKFIIDKKIPYTSPIRNLVAIEQPASSG
                     VKELRQRLAELI"
     gene            complement(8728..9390)
                     /locus_tag="VGQ51_10075"
     CDS             complement(8728..9390)
                     /locus_tag="VGQ51_10075"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEV9036960.1"
                     /translation="MGHFTERLRASVKQSGAYIAQEQLSQSASEIENTITVKVPVDRF
                     EDLMGELPSDSDKLVSKQVSSADVTIELVDTKSRLETKKEVRERYLDLLKQAHSMKDI
                     IAVQNEINDIQQEMDQASGRIAYLGHSSAYSTINLHYFQVLDVTAVETSSPTFFTKLK
                     DSIIEGWSGLSALLLQLVGVWPLWIALAIGIPWIRKYLRRSRLRTDAIRPPEETAGRP
                     GA"
BASE COUNT         2113 a         2598 c         2483 g         2310 t
ORIGIN      
        1 aaatcgaggg ggcatatgca agctttgcgg gatcgccgac gtcgcgaggg gaattccagt
       61 tcgatctctg gggagttgaa ccgacgcttc gctgggactg gtaccggctg aagagcgagg
      121 tcgtcaaaca cggcgttcgc aactccctgc tggtcgcgcc catgccgacg gcatccactt
      181 cccagatcct gggcaacaat gaatgtttcg aaccctatac atccaacatt tatgttcgcc
      241 gggtgctaag cggggaattc gtagtggtca acaaacacct cctgaaggac ctcgtggagc
      301 tgaacctctg gaatgacgac atgaagaaca agatcatcct ttgcaatggc tctatccaga
      361 cgatagacgg gatcccgcag gagatcaagg atatctataa gacggtatgg gagatcaaac
      421 aacgcaccat catcgatatg gccgccgacc ggggagccta tatctgccag tcgcagtcgc
      481 tcaatctttt tgtcgacgcc ccttcgtcta acaaattgac ttctatgcac ttctatgcat
      541 ggaaaaaggg cttgaagaca ggcatgtact acctgcggac gcaggccgca tcacaggctg
      601 tacagtttac ggtcgagcgg cagggcggaa agatggtgga gccgatggaa cctccggcgg
      661 gtgcgacttg ttcgatgcag gagggatgtg tcacctgcag tgcataaata tgaaaagggc
      721 ctccgaaatt ttcgggggcc cgcaagtata tttactattg caaacgaagc tttttgtttc
      781 tactttgtta atggttgttt aaatccaaac cctaaaatta aagccgtatg tttacgaaaa
      841 ccaatctcat ttccgccagc ctctttcttc tcgttgccat catccaaatg gcttcttagc
      901 gatcacttca gctgcatatc ttttatgatc ctgtcgatac ggtccgacaa ctcggcattg
      961 accttgtcgt attcggctgc atccgccaac ggaccatgta cgctgaaata gaatttgatc
     1021 ttcggttccg taccggaagg ccgtgctgag atcttgctgc catcctcgag gatgaactgc
     1081 aaaacattgg attccggaag ctcgaggtcc caggactctc cggtctggag gtttttgccc
     1141 ttgcgcagct cgtaatccaa taactcgacg accggtgatc cgttgatcgc ggccggaggc
     1201 tggctgcgat aggcgctcat cattgcggct atctctttct gcccgttcat tccttttttg
     1261 gtaatggaga tcaggctctc tttgtagaaa ccatattcca catagagatc gatcagcttg
     1321 tcataaagac tgcggccttt gtctttttcg tatgccgcca tttcgcatag aagtgctacg
     1381 gcactgaccg cgtctttatc cctgatctgg ctgccgatca tcagaccata gctttcctca
     1441 ccgccgataa catagttctc gcgaccttct ttttctttga tgagctcggc aatatatttg
     1501 aaaccggtga ggacattata gcaactgata ttgtttcgcc aggcgatctc atcgatcaga
     1561 ttggtggtaa cgatcgtttt gacgaccata tcgttggcag ccgcgacgcc cttcgccttc
     1621 ctggcttcga tcatatagtt gaaggccagc gtcgccgtct ggttcccgtt catcagaacc
     1681 cattgtccct tcttatcttt tacgccgatg cctacccgat ccgcatccgg atcggtgcct
     1741 aacaatatgt ccgcgtctat ctgttgcgcc ttgcgcagac cgatgctcat cgcttcgctt
     1801 tcctcggggt tgggatagat gacggtcggg aagttgccat cgggggtcgc ctgttcgtcc
     1861 acgacatgaa cattggaaaa cccaaaccgc tgcaatactt caggaaccag ctttattccc
     1921 gttccatgga tcggcgtata gacgatccgt agattgtgct gccgttcgat cacctcgggg
     1981 taaacgctca ggttgcggac cattccgata taggcctcgt ccatggcggc gccgatcatc
     2041 gagatattcg attcacctcc accccatttg acggcatcga caccggcaat cttttctact
     2101 tcctggataa cgttcttgtc atgcggcggc accagttgtc ccccgtcatc ccaatatgcc
     2161 ttgtagccgt tgtattcctt ggggttatgc gaggccgtac aaaccactcc gccctggcag
     2221 tgcaggtgcc ggatggcgaa actcagttca ggagtaggac gcagggactg gaaaagaaac
     2281 acctttatcc cattggccgc gaatacgtta gccacggttt cggcaaaaaa gcgactgttg
     2341 ttccggctgt catgggcaat ggcgaccctg acttccttac cgggaaagga cgctttcaga
     2401 taattggcaa atccctgggt agccatcccg acggtgtatt tgttcatccg gttggtaccc
     2461 actcctatga tgccgcgaag ccccccggta ccgaactcca ggttgcggta gaagctttcc
     2521 gtcaattcgt taggattttc tttttgcagg cgttggatct catctttggt cgcctgatcg
     2581 taattgccgt ttaaccattt atttactttg tccagtatag cagcgtccat tttccgatgt
     2641 tggtttgggg ccgaatttcg gaaaaaaaaa gtacatttgt acaagcctcc tacttcttct
     2701 tcaaatccat tgaaagcctt ctgccggtaa gtataacttg cctgtaacaa aatcgggtga
     2761 gataattgct attacttttc tgtaccgttt gatcgaggat cttaaccgga atatggatga
     2821 cgatgtatct gcttcttcgg aataacaagc aaagcggccc ctattctttg gatgatttaa
     2881 agaatatggg attgaaagcc tatgacctgg tatggttaga aggtaagagc gccgcatgga
     2941 gataccccag cgaaatagag gaattgaagg agttcgctcc ggctgtggaa gaacagcctt
     3001 tcgacaggtt ttataagaaa cccgcggcgg ccgctgtatc cgcctccaat ggcaatcagg
     3061 acaccactac catctccttc cggcctgcga ccgaggtctc cgccccggat gataaatccg
     3121 tgacgactca caaacgcatc atctatgtca ccctgccctc gggtaaacct gctccgcagc
     3181 cgatgcgcga agcgcccgta cgcgagatag aacgaccccg tcaactgatt cccgagcccg
     3241 taacgcccgt ttcagcgacc ccggccaccg gggattatcc ttccctttcc gaccataggg
     3301 ataggactcc tgtggaattc gcgccccgcc accggtctca tcaaaaagaa cggctctttc
     3361 gtgttgccgg catcgggggc agtatcctgg ccttgctggc ggcaggtata tttatcggct
     3421 tgtcactgaa caaagagtct ctcgggttac aaagacgaat tgcgtcgagg accacgcccc
     3481 agtctgttgc aaaacagact aatcctcaac ccacggttat acccgcggct aacgctgcta
     3541 tactggcgaa taccacggaa aaaacaaccg ctcctgtcgg ggacaatact atctcttcta
     3601 ccgtctccgc cgtcaggagg gtcagaaagc agaaggaaaa gatcccggcg gccctcatgc
     3661 cccccgttca gaaagattct gtcctcgcgg atattcccgt tgtaacgcgc gaggccgttc
     3721 accgtaccca ggcggaacct tccatcgata gcaaggaggc ggcaaagacg gccatcagca
     3781 gcgaggtatc cgtgggcgcc aatcgttatt ccgtcggtac tttcggcggc atcaacgatc
     3841 ttcagctgac agtatcgaat cgctctgcct atccactcga tctggttgtt gtagaagtcc
     3901 agtatattca agccaacaag aaggtgtaca aaacggagaa cctgtctttt cgcgggatca
     3961 gccctggcgc cgcgctcatg caggaagccc cgaagagttc gcgcggtatc cgggtacagt
     4021 ataagatcac ttccatcagc tcgaaggagc tggggctctc ctactccgga ttatgaaatg
     4081 gtgattaact atttgatcac taagattttc tcttattcct tctcatttgg cacagtagtt
     4141 gggtacaata gcgtgcctcg aacccccgca gttcaatttg cccattaaaa gcctgacgta
     4201 aagaccatct attcgtttac ccgtgagcat catcctggaa gccgcggtag acgactgtcc
     4261 atcatctgtc gaatctataa aacttgaacc ggtatgagaa gcgcgatcgt acaactgttt
     4321 ttggtcctgg taggcatttc cggcattcac gcagtaagat tattacaaac ttcttccttt
     4381 catgtccgcc tgttccccgc caatggggcc gggagggtct gggctatcca gggaaaggat
     4441 tccatcgaaa tgacgaatgt caacggggag tatatcctcc ggtcagtgaa acccggcaat
     4501 tgggaagtgt cggtgcaggc caacgggcct taccggagtg cttgtttcca gtcgggcgat
     4561 ctgaagcccg gtatggatag ggatctgggg gagatccgtc tcattcgatg agcagacggc
     4621 catgtgtagc cgatcatctc aaaaatttta agaaaacata ctaccttgtg gcggacaaaa
     4681 ttccttcatg aagcgtattc tcctgttcgg ggccggtaag tcggcgacca gcctgatccg
     4741 ataccttatc gatgtttgcg tgtcccgcga ttggcagctg acggtggccg aaaccaatct
     4801 cgatctggcc cggtcgaaga tcggccgggt gtcgcaggcg tctgccgtac agctgaacgt
     4861 tactgacgaa aacgagcggg acgcgttggt tgacggggcc gatgtggtga tttcgctgct
     4921 tccgcccaat cttcactatc tcgtggcgct ttcctgtctc aaaaagaaca aacatctgct
     4981 caccgcctcc tatctcgatt cggctatccg gacactggag ggcgatatac gtcggaaagg
     5041 gttgttcttt ctgggcgaga tgggactgga tcccggcatc gatcatatga gcgcgctgca
     5101 gctggtgcgc cggatcaaag acgatggtgg catcatccgc accttccact cgcatacggg
     5161 aggtcttatc gcccccgaaa gcgacgacaa tccctggcat tacaagatca gttggaaccc
     5221 ccgcaacgta gtgctggctg gttcggcggg agctacctac cgggagaaag gcaaggtcat
     5281 tacgcgcgat cagctatcgc tgttcgaacg acccggaacc gtctcattgg aggacttcga
     5341 catcggcccg ctggcctggt atcccaaccg ggactcgctc tcgtatatcc cgttatacga
     5401 tctggaggga acggagacct ttgttcgcac caccttgcgt tatcctgctt tttgccgcgg
     5461 ctggtccgcg atcgttcgcg ccggcctgac agatgataag gtacccgtcg atccgtccgg
     5521 gctcagctat gctgtctggt ccgcaccgct gcgtgcgctg gtcggtgtgg acaaccggga
     5581 acagctggac ttcctggggc ttttcgataa aggaccggtt cccaaaaccg cgcgtacttc
     5641 ggctgacatc ctgcaacccc tgttggagac gcgtcttgcc atgaagccgg ccgatagaga
     5701 tatgatcgtc atgatccacg aaataggctt cacgcaggca gaaagggctt ttatggcaag
     5761 aagcacgctg gtcgttaaag gagaggacaa tcttcatacc gccatggccc gcacggtcgg
     5821 gcttccgctg gggatagccg ccgtcctggt actggaaggc cggataaccc tgacgggtct
     5881 ccacatcccc atcctgcctg agatctatga gccggtactg gaagccctgg gtcgcgaagg
     5941 gatcgcattc ggggatacgg tgaagcctgt cgccccatcg ccgcacgcct agctcatccc
     6001 gatgctcagc tcgttataca gggaccacgg tctaattcac atcgatgctc agcgcactga
     6061 ggatccgata attgatatcc cggaaaaaga tcttgtattt ccccttccgg agatacagca
     6121 ggtaacgcac aatgccggta ggcgtcctcc cgccgggtgt ataggtctga tctgcgggca
     6181 ccagttgcca gggagtgttg tcattcgcat ctatttcttt ctgataatag gcctgtacga
     6241 agaacacctc tttcgaaggc gccttgatat aggtaggctg acgcaatccg tcgagactta
     6301 gccaggtggg acgaccgtcc agccaggtgg tgggtggatg gatgacgcat agatcgtaca
     6361 gatccttgtc ggtgacgttc accggcgcgt cgttgatcag cgcgatcgaa ggttccgtaa
     6421 gatggaattt ggtcgtatag gcctgataga tcacccggcc gtatccaaaa ctgctttcct
     6481 ccgtcatatc cgtctgatcg atggtcagcg gttctatacc cgacatacgc cagaaggcca
     6541 gtgccatcgg gatatgtccg tcgggcaggg cttttttgac gatatgggta agcgaggcat
     6601 gcacgagtat cttcgccttc ggatctttcc gcagcacttc aaagaggtgt gcggcctgga
     6661 cggaatcccg ctgatcagcc gtatgagcgc cggccagggt gtcctcgtac ggtacaagcg
     6721 taaagcccat cgcaagggcg gtgcgcatca attcgcccgc caccggttca cagacataat
     6781 atcccgaatg catgcctacc gaggttagcc ggcggttgga gaaattatcc agcatctcca
     6841 tggcgaggta ccggaacccc atccggtaaa aatccgccag cagcgataag gtaaatgccc
     6901 ggtgcagcgg tttggagaac gattcattga tcatcaccac ctgtctgtcc tttgccgcga
     6961 ggtggatata gcgacgggcg tcggcgtgta cgatattccc gagcccggcg gccgtgcggt
     7021 agatctttct gcgggtagca tcgtcgatcg tgtcatagct cctgaccagg tattgcaatg
     7081 cggtccggta gtctcccgcg aacgcgaaat actggcttac ctcgtcatag aagcctttat
     7141 ccgaatagat cttccggaac ttctcttcgt ctttcaacgc gtggtacagc gggaaaagat
     7201 agtgaaggtt gccgttgggc gtcagtgtca tgacattcat ccgcgggctg taacctgtgg
     7261 gggtactatc gcgacaatag ttgcgcaaac gagccaccga tgcggagagt tcgtcctcct
     7321 gcgacaaacc ttgcagagtg gaaagtaaaa gacccgccag taacataaac cgtaatgctg
     7381 ccatgccggc gcaaaatagg acaagtcttg ttaaaagaat ttgaaaatcc tggccggcac
     7441 tcagagtgtg gccagattct tttgccgcag ccttcgctta tagcctgcga gtttcaccca
     7501 gtagacgatc cagcaaataa tgccggccag ctcggtccag ccgatcagtt caatcaatcc
     7561 gatcgggacc ttaggattac cccataacac gggattcaac ccaaccccga tcacgataag
     7621 cgtgcaatac gcgatcccga tcgcctgggt aggccgtttg ttcccctgtg caatcgccgc
     7681 ggcctcgata cccaggattg aatcgttttc ccaggaggcg acctcattgc gtatggagcc
     7741 tgcgatccgg acgaccacga taaactgcca tatctggcca aagatgggga tcatttgaag
     7801 ccatacttgt ccggggcgaa tccgccggtt ctcgttctta atcgttcgta aagtgttttg
     7861 ttcggtaagc aggaagagga tcgcgatgat gaggaagact cccagaagaa caagctggag
     7921 ccagcccccg gtttcagaag cagttgtttc cacggcaagt aatttgagct aatttaggag
     7981 atttcagatc aattcggcca gacgctgacg caattctttc acgcctgagg aagcaggttg
     8041 ctctatcgcg accaggttcc ggatgggaga cgtatagggt atctttttgt cgatgatgaa
     8101 tttcggaatg cccggcatcg ccagatcgac cagacctgcc gcgggataca cgaccagcga
     8161 ggtacctacg atgacaaaga tgtctgcggt cctgaccaac gggacggcat atccgatcat
     8221 gggtacgggt tcctcgaacc agacgatatg gggtcgtagc tgcgctccgt cctccgcccg
     8281 atgaccgagc aggatatctt cgcggatatc gtagaccagt gtctcgtctt tttcgctgcg
     8341 catctgaaag atcgagccgt ggagatgcat cacactggta gaacccgccc gttcatgcag
     8401 atcgtcgatg ttctgggtga tgatatgtac atcgaaatcc ttttccagct gcgccagacc
     8461 ggtatgccct tcattaggcg ccgccttcag cgtatccctg cgccgcatat tgtaaaagtc
     8521 gagcaccagc gcgggatctt ttcgccaggc ccgcggcgta gccacttccg tcacgtcata
     8581 tccctcccag agaccgtccg agtccctgaa cgtcttcagc ccgctgtctg cgctgatgcc
     8641 cgcacccgtg agcaccacca gcttcttctt tgccatatcg caatgtaaaa aaaactcccc
     8701 acatgccgcg gggagtttgg cgtagcctca tgcgccgggt ctgccggctg tttcctcagg
     8761 ggggcggatc gcatcagttc tcagccgcga tctgcgcagg tattttcgta tccagggaat
     8821 cccgatcgcc agagctatcc acagcggcca taccccgacc agttgcaata ataatgcgct
     8881 taacccgctc cacccctcga tgatcgagtc ttttagtttg gtgaaaaatg tgggcgaaga
     8941 agtttccacc gctgtaacgt ccagcacctg aaaataatgc aggttgatag tgctgtaagc
     9001 cgaggaatga ccgagatacg cgatacgtcc ggaagcctga tccatctcct gctggatatc
     9061 gttgatctcg ttctgcactg cgatgatgtc tttcatgctg tgtgcttgtt tcaaaaggtc
     9121 caggtatctt tcgcgcactt cctttttggt ttccagcctt gactttgtgt cgactagctc
     9181 tatggtgacg tctgcagagg agacctgctt cgagaccagt ttgtcactat ccgaaggtag
     9241 ctctcccatt aagtcttcga aacgatcgac aggtacttta acggtaatgg tgttctcgat
     9301 ctcgcttgcc gattggctca attgttcctg cgcgatgtac gcaccggatt gttttacgga
     9361 agcacgcaga cgctcggtga aatgacccat atcgtttagt taagaacact acctgttaca
     9421 agtctttgta attcatatgg tataatcgcg gcggtctttt cttgcgcggg ctttttctgc
     9481 cctggcgatg agcctctgtg ttcg
//
LOCUS       DASYSV010000335         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_65750, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000335 DASYSV010000000
VERSION     DASYSV010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65750"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VGQ51_10080"
     CDS             <1..1098
                     /locus_tag="VGQ51_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEV9036961.1"
                     /translation="AMRSSSPSSSRPVAIWVYTGVIMLTIQVILGGITRLTGSGLSIT
                     HWDVATGVIPPLNQAQWQQAFDGYKQTPQFQILNSDFTLTNYKFIFFWEWFHRLWARL
                     VGVVFLVGFAWLLWKRKLQPRMVRPLVILFLLGALQGVIGWIMVASGLIGDAIYVEPT
                     RLALHFIFALVLIVYTFWFALMLSVPDERRLAPGVAGVAGLRRWTVALLVLLFFQLLY
                     GALMAGHKAANVAPTWPDINGSFMPDSVFTQRPLLRDLAGNRITVQFIHRSLAYLLLA
                     GVICWTIAASRLKQATSGVFRRLRWAPLAVITLQVVLGISSLLTSPAIIPHRWVAFDW
                     FAQFHQITGLLFLLLMIGMLYLVIPVRTRSV"
     gene            1095..3338
                     /locus_tag="VGQ51_10085"
     CDS             1095..3338
                     /locus_tag="VGQ51_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036962.1"
                     /translation="MMRILKAVYYFFPLQLLLLHLRKFQVLLIFWLILFSTVDGGFLR
                     AYGADALFLSPEYLGDVSPISASIVGVATGIFIMSWNITTFIIFSRHFRFLATTGKPF
                     LKYCINNTILPAIFLIFYFIRVLQFSTSRELLPLHSIVLLCAGFIAGLAFIVCASFLY
                     FFRADRTIIRKMTPVISNPRLFKSQFSKEEVKLNESRLMRVKWYLNSPTSIRKTRDVA
                     HYSKAFIESIFNRHHIAAVLSIFVAFIFLIVVGFFMDSRFFQLPAAASILIFFAILIS
                     LSGAFSYFLQSWSIPILLLLFFLLNFLYRHNVIDPTNKAYGLDYSTNVDRPLYDRETL
                     LQLCSEKNVERDKANMLRILEKWKARQSSPKPTLFLVNTSGGGSRSATFTFSILQRLD
                     SLSGGQLMRKTFLVTGASGGMLGAAYFRELARRRDEGDSSIHLRDYKYVDDISRDLLN
                     TLFSSFVARDLASPAQEFTVDGHDYVKDRGFAFEQRLDHNTHGLLDLQLKDLAPYESS
                     AREPLMFFNPVITRDSRAMIISTQPVSFLMRPRYDSTRIPPMDPDAVDFSALFAHQGP
                     MNLRLLTALRMNATFPYVLPNVWLPTRPVIDVMDAGLRDNFGEMDALRFLNAFREWLQ
                     QNTDGVVLLQIRDRKAGGWENPYESNDITEIVTKPLLLLQDNWYKLQEFSQDDLLSMA
                     QKGMGFPFRKLIFQYVPKTEYEGAALNFHLTKEEKQNISGSLDNLDNKHAFGEFVQLM
                     RAPQPSH"
BASE COUNT          680 a         1012 c          878 g          855 t
ORIGIN      
        1 gctatgcgat cctcttctcc ctcttcttcg cggccggtag ccatctgggt atacaccggc
       61 gtgatcatgt taactatcca ggtcatctta ggcgggatca cacgtctgac cggctcgggt
      121 ctttcgatca cccattggga cgtcgccacc ggcgtcatcc ctccactgaa tcaggcacaa
      181 tggcaacaag ccttcgatgg atacaaacag accccgcaat tccagatcct caattcggac
      241 tttaccctga ccaattataa attcattttt ttctgggaat ggttccaccg gctatgggcg
      301 cgtctggtag gtgtcgtctt cctcgtcgga ttcgcatggt tgctctggaa acgcaagcta
      361 cagccccgta tggtacgccc cctggtgatc cttttcctgc tcggggcgct gcaaggcgtc
      421 atcgggtgga tcatggtcgc cagcggtctc atcggcgatg ccatttatgt agagccgaca
      481 cgcctcgcgc tccattttat cttcgcgctg gtgttgatcg tatacacctt ttggtttgca
      541 ttgatgctgt ccgtgccgga cgagagaagg ctcgcccccg gggtggccgg ggtcgccgga
      601 ttgcgtcgct ggaccgtcgc attgctcgtg ctcctttttt tccagctatt gtacggtgcc
      661 ctgatggcag ggcataaagc ggcgaacgtc gcacctacct ggcccgatat caatgggagc
      721 tttatgccgg acagcgtgtt cacccagcgg ccgctcctcc gcgatcttgc gggtaaccgg
      781 atcaccgtcc agttcatcca tcgtagcctg gcttacctgc tgctcgcagg cgtcatttgc
      841 tggaccattg ccgcttcgcg gctaaagcag gcgacatcgg gggtttttcg gcggctcaga
      901 tgggcgccgc tggcagtcat caccctccag gtcgttttgg ggatcagcag cctgctgaca
      961 agcccggcga tcatcccaca ccgatgggtg gccttcgact ggttcgcaca gttccatcag
     1021 atcacaggtc tcctattcct attgttaatg atcgggatgt tgtaccttgt gatacctgtt
     1081 agaacaaggt ccgtatgatg cgaatcctaa aggctgttta ttatttcttt ccgctacagc
     1141 tccttctatt gcacttaaga aagttccagg tcctgctgat attctggttg atactcttca
     1201 gcacggtaga tggaggtttt ttacgcgcct atggcgcgga tgcgcttttc ctgtctcctg
     1261 aatacctggg cgacgtcagc cccatcagcg cctccatcgt aggagtggcc acaggcatct
     1321 tcatcatgag ctggaacatc accacgttca tcatcttcag ccgtcatttc cgtttcctgg
     1381 ctaccaccgg caaacccttt ttgaaatatt gcatcaacaa taccatcctg ccggctatat
     1441 tcctgatctt ttactttatc agggtactgc agttcagcac cagccgcgag ttgcttccgc
     1501 tacattccat cgtgctgctc tgcgccggtt ttatcgccgg gctggctttc atcgtttgcg
     1561 cctcatttct ctattttttt cgcgcggaca ggaccatcat ccgcaagatg acacccgtga
     1621 tcagtaaccc caggctgttc aaatcgcagt tcagcaagga agaggtcaaa ctgaacgaaa
     1681 gccgtctcat gcgcgtgaaa tggtatctca actccccgac ctccatccgg aagacccgtg
     1741 atgtggcgca ctatagcaag gccttcatcg agagcatctt caaccggcac catattgccg
     1801 ccgtgctgtc tatcttcgtg gctttcatct ttctcatcgt cgtggggttt ttcatggaca
     1861 gccggttctt ccagttaccg gccgcggcaa gcatcctcat cttcttcgcc atcctgatct
     1921 cgctgtccgg cgccttctcc tatttcctcc aatcctggag tatccccatc ctcctgctgt
     1981 tgttcttcct cctcaatttc ctgtatcggc ataacgtcat cgatccaacg aataaggcct
     2041 atgggctcga ctacagcaca aacgtggacc gccctctcta cgaccgggag accctgctgc
     2101 agctgtgctc cgaaaagaac gtcgaacgcg ataaggccaa tatgctgcgt atcctggaaa
     2161 aatggaaggc ccgccagtct tccccgaaac cgaccctttt cctcgtgaac accagcggcg
     2221 gtggaagccg cagcgcgacc ttcacgttca gcatcctcca acgtctcgac agcctcagcg
     2281 gaggacagct gatgcgaaaa acctttttgg tcaccggcgc ttcgggagga atgctggggg
     2341 cggcctattt ccgtgagctg gcccgtagac gggacgaggg ggacagctcc attcatctcc
     2401 gtgattacaa gtatgtcgac gatatatcgc gcgatctgct taacactctt ttttcttcct
     2461 ttgtggcccg cgatctcgct tctccggcgc aggaattcac ggtggacggg catgattatg
     2521 tcaaggaccg tgggttcgcg ttcgagcaac ggctggatca caatacccat ggattgctcg
     2581 atctgcagct caaggacctt gcgccatacg agtcgtccgc gcgcgagccg ttgatgttct
     2641 tcaatcccgt catcaccagg gacagccggg cgatgatcat ttccactcaa ccggtcagct
     2701 tcctgatgcg tccacgttat gacagcaccc ggataccgcc gatggacccc gacgcagtcg
     2761 atttttccgc cttgttcgcg caccagggtc cgatgaacct ccgcctgctg acggccctgc
     2821 gaatgaatgc cacgtttccc tatgtcttgc ccaatgtatg gctgccgacc cggcccgtca
     2881 tcgatgtcat ggacgctggt ctccgggata atttcggtga gatggatgcg cttcgcttcc
     2941 tcaatgcatt ccgggaatgg ctgcaacaga acacggacgg cgtggtgctg cttcagatcc
     3001 gcgacaggaa agcgggtggg tgggagaatc cctacgaatc caacgacatc accgagatcg
     3061 tcaccaaacc gctgttgctg ttacaggaca actggtacaa gttgcaggag ttcagccagg
     3121 acgatctgtt gagtatggcg caaaaaggga tgggttttcc gttccgcaag ctgatcttcc
     3181 aatatgtgcc gaaaacggaa tatgaagggg cggcgttgaa tttccatctc accaaagaag
     3241 aaaaacaaaa tatctccggg tcattggata atttggataa taagcatgcc tttggtgaat
     3301 tcgtgcagct gatgcgggcg ccgcagccgt cgcactaagg ctggatgagc cggatagcct
     3361 cccgcaccac ctcgatccga aaactggcag cggaattggc cgggtccccg tcgatatgca
     3421 atggc
//
LOCUS       DASYSV010000336         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2242975, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000336 DASYSV010000000
VERSION     DASYSV010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242975"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VGQ51_10090"
     CDS             complement(<1..496)
                     /locus_tag="VGQ51_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024987.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4140 domain-containing protein"
                     /protein_id="HEV9036963.1"
                     /translation="MRLSILLFSCALVFCTIRASAGEEPATVPSTLKSVMVYRNGAEL
                     THSATARLEKGNNQLVITDVSNAIDASSIRIKCSASVTVLSVAVSTDYLIEEAASPVV
                     RRLQDSIGAIEKELSRLAVMTKTDNEMLELLGSNKSIGGSTSGVSVAELAKMMDYYRQ
                     KSIEL"
     gene            complement(551..2206)
                     /gene="glgP"
                     /locus_tag="VGQ51_10095"
     CDS             complement(551..2206)
                     /gene="glgP"
                     /locus_tag="VGQ51_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEV9036964.1"
                     /translation="MSFKNYVVPYAVDERYSKKVAYFSMEFAVHQPLKIYSGGLGFLA
                     GSHLRSAYELRQNMINIGILWKYGYYDQTRNQDQTLAVSWMEKQYSFLEDTGIRFQVN
                     IHDHPVWVKVWYLNPETFQSAPLFLLSTDVPENDYVSQTITHRLYDANVATKVAQFIL
                     LGAGGAKLVDELGFNPDLYHLNEAHAISSAFYLYQKYNRDIEEVRKRLVFTTHTPEEA
                     GNEKHDIYLCHKMSYFCGLSIDEVKKLAGLEDDAFNHSLVALRFAHLANGVSQLHGVV
                     SRALWSKYKNICPIIAITNAQNWRFWADKELYRKMEEGNDYWWDDRKKYLKKRSFEIV
                     ADQTGKLFDPNVFTIVWARRFAGYKRAGLLTRDMDRFNRLLNDKKYPVQIIWAGKPYP
                     MDYPAINEFNHLVHLSKGYKNVAVVIGYEIGLSKRLKQAADCWLNNPRVPREASGTSG
                     MTAAMNGAVNFSTDDGWIPEFVNHGNNGFVVPKTDYASMHVEQVDDYDLDHLYAILDK
                     EILPLYYDNYDTWRQIAKNGMRDVRYQFDAGRMAHEYYEKLYK"
     gene            complement(2339..>2602)
                     /locus_tag="VGQ51_10100"
     CDS             complement(2339..>2602)
                     /locus_tag="VGQ51_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036965.1"
                     /translation="FLFFVHFEKDDGTCKGELKGEAKFVSPGVARYRSYSDPCAIEFT
                     FIPEGVSLKEIGGCGVHRDIRCYFEGYFERRKTAHGPPKTHHN"
BASE COUNT          600 a          722 c          649 g          631 t
ORIGIN      
        1 gcagctcgat cgatttttgc cggtaataat ccatcatttt ggcaagctcg gccacggaga
       61 cgccgctcgt actgccgccg atgcttttat tggacccgag cagctcgagc atttcattat
      121 ccgtctttgt catcacggcc agccgggaaa gctctttttc gatggctccg atagaatcct
      181 gcagccgcct gacgaccggg ctggcagcct cttcgatcag ataatccgta gaaacagcga
      241 cggataggac cgtaacgctc gccgaacatt tgatccggat gctggatgca tctattgcgt
      301 tgctgacatc ggtaatgacg agttgattat tgcccttctc gagacgggcc gtggccgaat
      361 gcgtcaattc cgcgccatta cggtagacca tgacggactt caaggtggat gggaccgtgg
      421 ccggctcctc ccccgcgctc gcgcggatcg tacaaaacac caacgcacag gaaaataaca
      481 agattgacaa tcgcatagag tgattttcca ctcaggatgc aaaaagtcgc cggattacac
      541 aatcgggcac ctatttgtac agcttctcgt aatattcatg cgccatccgg ccggcgtcga
      601 actgatatcg gacgtcccgc atcccgttct tggcgatctg ccgccaggtg tcataattgt
      661 cgtaatataa aggaaggatc tctttatcca gtatagcgta caggtggtcc agatcgtaat
      721 cgtccacctg ctcgacatgc atcgaggcat aatcggtctt tggcaccacg aatccgttgt
      781 tgccgtggtt gacgaattcc ggtatccagc cgtcgtccgt cgaaaaattc acggcgccat
      841 tcatggcggc cgtcatgccg ctggtgccgg aagcttcgcg cggaacacgc gggttattca
      901 gccagcagtc ggcggcttgc ttcagccgct tggataaccc tatttcatag ccgatgacga
      961 ccgcgacgtt cttatacccc ttactcaggt gtacaagatg attgaactca ttgatcgcag
     1021 gataatccat gggatagggc ttgccggccc agatgatctg aacgggatat ttcttgtcgt
     1081 tcaacagacg gttgaacctg tccatgtcgc gggtgagcag accagcgcgt ttatacccgg
     1141 cgaaccgccg cgcccagacg atcgtaaaaa cgttgggatc gaacagcttt cccgtctgat
     1201 cggcaacgat ctcgaagctt cttttcttca ggtatttctt acggtcgtcc caccagtaat
     1261 cattgccttc ctccatcttg cgatacagct ccttgtcggc ccagaaacgc cagttctgcg
     1321 cattggtgat cgcgatgatg gggcagatat tcttgtattt gctccatagc gccctggaaa
     1381 cgacgccatg cagctgcgaa acgccattgg ccaggtgggc gaaccttagc gccaccagcg
     1441 agtgattgaa ggcatcgtct tccaacccgg ccagcttctt cacctcatcg atcgacagtc
     1501 cgcagaaata gctcatcttg tgacagagat agatgtcgtg tttctcgttg ccggcctctt
     1561 cgggcgtatg cgtggtaaaa acaagacgct tcctcacctc ttcgatatcg cggttgtatt
     1621 tctggtagag ataaaaagcg ctgctgatcg catgcgcctc attgagatga taaagatcag
     1681 gattgaaacc cagttcgtcc accagcttgg caccgcctgc cccgagcagg atgaattgag
     1741 ccactttagt agcgacattg gcatcatata atcgatgcgt gatcgtctgg gacacatagt
     1801 cgttctccgg tacgtccgtg ctcagcagga acaggggcgc gctttggaaa gtctccggat
     1861 ttaaatacca gaccttgacc cataccggat gatcgtggat gtttacctgg aaccggatgc
     1921 ccgtatcttc gagaaaacta tattgctttt ccatccagga gaccgccagc gtctgatcct
     1981 ggttgcgcgt ctgatcgtaa tatccgtact tccataggat gccgatattg atcatattct
     2041 gacgaagctc gtacgcgctg cgcagatggg aacccgccag aaaacccaga ccgccactat
     2101 agatcttcaa gggctgatga acggcaaact ccatcgaaaa ataggctacc ttcttggaat
     2161 accgctcgtc cacggcgtaa ggcactacat aatttttaaa gctcatagcc aaaccaaatt
     2221 gtttttacct gtgtaaaaag tacgcaatat aagaatttct cacttttggc gaactatccc
     2281 ctgccccttg ccgctcccgg gcaatgcccc ggggcgataa taacggctaa accggatttc
     2341 aattgtgatg tgttttaggg ggtccatgtg cggtcttcct gcgctcgaaa tatccctcga
     2401 aatagcaccg gatatcccgg tgtacgccgc atccgcctat ttctttcagc gagaccccct
     2461 cggggataaa ggtgaattcg atcgcacagg ggtcggaata ggacctgtag cgcgcgacac
     2521 ccggcgaaac aaactttgct tcccccttca gctcgccctt acaggtgccg tcgtctttct
     2581 cgaaatgcac gaagaacagg aa
//
LOCUS       DASYSV010000337         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1219046, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000337 DASYSV010000000
VERSION     DASYSV010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219046"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VGQ51_10105"
     CDS             <1..202
                     /locus_tag="VGQ51_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEV9036966.1"
                     /translation="EISHPQHVHRNEEIILLRSGHVRMHIANSYLDAAGGDLVFLPSG
                     VPHDLENGKDSSCEYFALQWEP"
     gene            219..2348
                     /locus_tag="VGQ51_10110"
     CDS             219..2348
                     /locus_tag="VGQ51_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036967.1"
                     /translation="MKFLIATIFLIPLAGRCQSPPDWQYDRHRPAAVYAAARLMTSGT
                     RHLSIRLIIDSVHLEKEAFAIEPAGRTITIKGGDDRGLIYGSIALSETIARGIPPDKI
                     RPSHDHPHFPFRAIKFDLPWDTYRHSYALDQHYATCRDTLFWKAFLDMMVNNRFNALC
                     LCNLHPYPYMIRARNFPEACPFSDSELKEWQTLFHSIFRMAKERGIDTYLVPFNIFVS
                     PEFARTHSVELNNLQHHFFVDGDTSEIIRRYTRESVTQVLQEYPDLTGFGLTLGEGMG
                     GMTPQKREDWMKETIIAGMRDAGRKTKLIHRIPFSGGTGSLGLTSIETERLTRKAIDE
                     EGTFDFLEPPIWAGLKYNWSHAHSTTRLIKVHGGRLYDTYFKPLPTSYKIVWTARNED
                     FFCLRWGVPGFIRAHIKTNSQPYTGGYIVGSETYIPAKDYFTNPGLLAHADWKYAFQR
                     QWLFYMLWGRLLYDPDTPDSIFRGEFIRRYGPRAAPLLEACALAGQTPLRLASYFDFT
                     WDFSLYSEGFLALDTATKSVDYIFVDRLIDHPVTDPAYVPVKEYVDSLLAHKRISSRI
                     TPMRLADDLAKDCIKAVRLVNDIPIANSTSLLYEVTDVKVWASLGLHFAHQLQGAVAL
                     EKFRRTGDTASRDLAIEYLQLALDDWDEVIRLTRPLYNDMPLTHLSEQNGVRSPENAR
                     LRWHWAMTRAAVARDVEIARTARPYSP"
     gene            2370..>2716
                     /locus_tag="VGQ51_10115"
     CDS             2370..>2716
                     /locus_tag="VGQ51_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV9036968.1"
                     /translation="MKPKLLKVPRTPSHSFSVREDRDPFINNRWHYHPEVELIIFHQG
                     SGTQFVGDHIKRFQPGDIILVGSNLPHYWRYDDVYFIEDAPRPVHSTVIHFSENFWGE
                     AFLHLPETKPLRNL"
BASE COUNT          602 a          913 c          637 g          564 t
ORIGIN      
        1 cgagatcagc cacccgcagc atgttcaccg gaatgaagag atcatcctcc tccgcagcgg
       61 tcatgtccgg atgcatatcg ccaatagcta tctcgacgcg gccggcggcg accttgtctt
      121 cctgccctcc ggagtaccac atgacctcga aaacggcaaa gactcatcct gtgaatattt
      181 cgcgcttcag tgggagccat aatcacatct atcgccatat gaaattcctg atagccacca
      241 tcttcctcat ccccctggca ggccggtgcc aaagtccccc ggattggcaa tacgaccgcc
      301 accgtcccgc ggcagtctat gcggcagcgc gtttgatgac atccgggacc cgccacctct
      361 ctattcgcct catcatagac agcgttcacc ttgaaaagga agctttcgcc atcgaacccg
      421 caggcaggac catcacaatc aaaggcgggg acgacagggg cctcatctat ggcagcattg
      481 ccctttccga aaccatcgcg cgcggaatac ctccggacaa gatccggccg agccacgacc
      541 atcctcattt tcccttccgt gccatcaaat tcgatctgcc ctgggatact taccggcata
      601 gctatgcgct ggaccaacac tacgccacct gccgggacac cctcttctgg aaggcctttt
      661 tggatatgat ggtaaacaac cgcttcaatg ctctttgcct ttgcaacctc cacccctacc
      721 cgtatatgat ccgcgcccgc aacttcccgg aagcctgccc cttctccgac agcgaattga
      781 aggaatggca gaccctcttt cacagcatct tccgcatggc aaaagagcgc ggcatcgaca
      841 cctacctggt cccgttcaat attttcgtca gccccgaatt cgccaggacc cacagcgttg
      901 agctgaacaa tctccaacac catttttttg tcgatggcga cacctcggag atcatccggc
      961 gctacacgcg ggaaagcgtc acccaggtgc tgcaggaata cccggacctc accggtttcg
     1021 gtctgacgct tggtgaagga atgggaggca tgactcccca aaaacgcgag gactggatga
     1081 aggagaccat tatcgccggc atgcgcgacg caggtcggaa gacgaagctc atccaccgca
     1141 tccctttctc cggggggacg ggctcattgg gcctcaccag catcgaaacc gagcgcctca
     1201 cccggaaagc catcgacgag gagggtacgt tcgattttct cgaacccccg atctgggccg
     1261 gtctgaaata caactggtcc cacgcccatt ccaccacccg gctgatcaag gtgcacggcg
     1321 ggcgactata tgacacctat ttcaaacccc ttcccaccag ttacaagatc gtctggaccg
     1381 cgcgcaatga agatttcttc tgtctccgct ggggagttcc cgggttcatc cgcgcgcata
     1441 taaagaccaa cagccagccc tatacgggtg gttatatcgt gggctccgaa acctatatcc
     1501 ccgccaaaga ttatttcacc aatcccgggc tgctggctca tgcggattgg aaatacgcct
     1561 tccagcggca atggctcttc tatatgctct ggggccggtt gctttatgat cccgatactc
     1621 ccgattcgat cttccgcggt gaattcattc gccgttatgg cccacgggcg gccccattgc
     1681 tcgaagcttg cgccctcgcc ggccagacac ctctgcgtct cgcctcctat ttcgatttta
     1741 cctgggattt cagcttgtac agcgaaggct ttcttgcgct ggatacggcc acgaaaagcg
     1801 tcgactatat cttcgtcgac cgcctcatcg accatcctgt caccgacccc gcctatgtgc
     1861 ccgtgaaaga atatgtagac agcctgctgg cgcataaacg catttccagc cgcatcactc
     1921 ccatgcggct cgccgatgac ctcgcaaagg actgcattaa ggccgtccgc ctggtaaatg
     1981 acattcctat cgccaattcc acatccctgc tctacgaagt gacagacgta aaagtctggg
     2041 cctccctcgg tcttcatttc gcccatcaac tacagggcgc cgtcgcgctc gaaaaattcc
     2101 gccgaacggg agataccgct tcccgcgacc tggcgatcga atatctccaa ctggccctcg
     2161 acgactggga cgaggtcatc cgcctcaccc gccccctcta caacgatatg cccctcaccc
     2221 atctcagcga acagaacggc gtccgttcgc ccgaaaatgc gcgcctccgc tggcattggg
     2281 ccatgacaag ggcagccgta gccagggacg tggagatcgc ccggaccgcg agaccgtact
     2341 caccctaaaa tcctaccttt gcagacagca tgaagccgaa gctcctaaaa gttccacgga
     2401 caccgtccca ttcctttagc gtcagggagg accgcgaccc gttcatcaac aaccgatggc
     2461 actaccaccc cgaggtcgag ctcatcatct tccatcaggg cagcggcact caattcgtcg
     2521 gcgatcatat caaacgcttt caacccggtg acatcatttt agtaggcagc aacctgcccc
     2581 actactggcg gtacgatgac gtctatttca tcgaagacgc cccgcgcccc gtccattcga
     2641 cggtgatcca tttctccgaa aatttctggg gcgaagcctt tctccatttg cccgaaacca
     2701 aaccgctccg caacct
//
LOCUS       DASYSV010000338         8492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1474048, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000338 DASYSV010000000
VERSION     DASYSV010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474048"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3348
                     /locus_tag="VGQ51_10120"
     CDS             <1..3348
                     /locus_tag="VGQ51_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEV9036969.1"
                     /translation="DGRPRNIIFILTDDHRYDALGFLKSQPFIKTPNLDRLAQGGAYL
                     KNAFVTTSLCSPSRASILTGLYAHKHKVVDNNNPVPKDLVFFPQYMQQIGYQTALVGK
                     WHMGGDFDDPQRGFDYWVSFKGQGSYLPEKNGLNVNGKHVPQKGYITDELTDYALDFL
                     KSRDRNKPFMLYLSHKAVHADFIPADRNKGMFKGYPFHPPLTMQAGTQEGAPMWVQNQ
                     RNSWHGVEFPYHSSLDIGEYYRRYAETLYGVDESVGRIMAYLKEQGLLESTLIIYMGD
                     NGFQFGEHGLIDKRTAYEASMRVPMLAWCPELIKPGTVVKEVVANIDIAPTLLDAAGL
                     RAPDYMDGKSFLPLLEGKKEPWRDGLLYEYYWERNYPQTPTMHAIRGDRYKYIHYNGI
                     WDTDELYDLQNDPLETRNLIRDKACQDIVKKMNHELFQQLEESKGMYIPLNPDRGEQS
                     NLRNRYGSPAAAFPDYLLRGDTISAFPATIQLSPADSGKQRPAAMRESIDDIIKKGFG
                     HFWVYGSSADANKEALDYAESRGMYVDYMTSGFEGFDRDHPPVMSVYDPQYASEVQKR
                     VAAGLAPMKALKRVYAVFPFQDEPFHAGASAFDYSSFARAEFAKRYGYTMPDSLGAVK
                     NDPKKWVDLLNFQSGTFSDGWKKVYDAVKAFDPRPGIVMTHDSHNTFGAGVKSNSKIA
                     MDDVFHWGGDFADILAYDIYPYMTFDYRYGEPGRLRKPRISQMHYTIAQLRNVCTTYG
                     KQMGFWVGTYSDNWFQRFKGPERKSQYWSEHEMAYTAIAQGANFLISPSNYNTTDLPL
                     DSMHWADYGKAMSVIQQAGPGLLKAPRVKARAAFLFPRSQYLLLREEYFNVGLTYEAF
                     LRAFGELDILHEDQITDDKLDGYRVLVLADVKLLPAKVAGYIASFVRNGGIVIADCVP
                     NMDDRKRPLEGMATLFGVSHAATDRVKEEGQWVPFSAIPPKMSFPPAGKASAADEKEA
                     RTDRVEGTAFQQHFVFPVVSPHGCEVKGGRVVLKMRSGRPALMCRQTGKGKTWLFGFS
                     MQDTYFNTWKNKDSAGRQELEDLIGDVFRESKIQAHINSSNPDIEASVRANSNEGYVF
                     VINHEAVNPGTTVRIADLNFKLAISST"
     gene            3473..3862
                     /locus_tag="VGQ51_10125"
     CDS             3473..3862
                     /locus_tag="VGQ51_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEV9036970.1"
                     /translation="MKKIVHPDRDPGFNTGAYSDGVMVDGFLFVSGQAAVDFKTSKFI
                     LGSIEEETHRTMQNIRSIVQAAGASMGQVVKCTVHLSDIKDFDRYNEVYATYFPGIKP
                     ARTTVQSVLAEGIKVEIDCIVRCTGIA"
     gene            3880..4869
                     /locus_tag="VGQ51_10130"
     CDS             3880..4869
                     /locus_tag="VGQ51_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEV9036971.1"
                     /translation="MGKIQTEDIEVGVVGLGLMGTSFIASLLIAGHRVKAVAPLPDDM
                     RAAPQRIKNHLIACERAGLLCKPIDSYLAKLLIAEDYEMLKSCHLVVECVIEIAGIKR
                     SIYEKIERVVAKDAIIASNTSAIPISELQKDLVNPARFLGIHWAEPSYFTRFIEITNG
                     AQTAIHYAEWVYALAHFWGKEPTLLRKDVRGFITNRLMYAAYREGLSIVSKGEATLED
                     VDKAFRYDAGSWMTLMGVFRRMDFLGLKDFLEIFRRTFPQLCNDEEVPALMQKMVDIN
                     ARGVQNLSGLYAYSEEEAAKWNEAFALFNVDIFRLAASYPATLMAQTASITNF"
     gene            4882..6186
                     /locus_tag="VGQ51_10135"
     CDS             4882..6186
                     /locus_tag="VGQ51_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV9036972.1"
                     /translation="MEKYSYTESAALLARARKALAGGVSSEFRKYNHPHAIFYNHGEG
                     SYIYDVDGNKYLDFTLSQGPLILGHSHPEVLKAVAEYSRQGQLFAGQHIKEIELAEKL
                     QELISSAELIRFCLDGSTAVQTALRVARANTGRQKFLRFEGHYHGWLDNVSWGISASS
                     AEAMGSREQPKVFPWTEGLPERARDEFIILPWNDLELVKKVVAERHKEIAAIITEPVM
                     CNNSCILPAEGFLEGLREICDEYGIALIFDEVITGFRMSLGGAQQYFNVIPDLSIFAK
                     AMGSGYPISAVVGKRKWMNLIQDARVIHAGTMNAGNPTVAAALATTSVLEAERPYERM
                     FLYGQRLMDGLRQAAAAAGQKLLVQGPGPMFHMGFTHLDKVSDYRDSFNYDKAKLGKF
                     IAGMHDRGVRVIGRGLWYISAVHTEEEIDTAIKAASEVMQTI"
     gene            6197..7258
                     /locus_tag="VGQ51_10140"
     CDS             6197..7258
                     /locus_tag="VGQ51_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036973.1"
                     /translation="MSDRRGFIKNVSGLGLLGAAPGLLSPRANSFRPQAGAGDGKIWG
                     CLLHLSYNFWVEYSTPSPFRGYRPYLQMSEPLWNDATEKMVQEGLNLVVIDLGDGVRY
                     ESHPEIAVNNAWSTTRLRKELERLRKMGLEPIPKLNFAAGHDIWLGKYSRMVSTDKYY
                     EVCGDLIEEVSGLFEKPRFFHLGMDEENAEDQRYLDYVVIRQNDLWWHDLYFFVDRVE
                     KCGSRSWVWSDYLWHKPEAFFKKMPKSVLQSNWYYDEKFDEKLTPVKAYLDLEADGYD
                     QVPTGGFYRKDGTGEKSIRNTVEFCAAHIADPRLYGFLQTVWAPTTEDNRESILKAIR
                     LAGEAKNWFDKDHNKPAKA"
     gene            7261..>8492
                     /locus_tag="VGQ51_10145"
     CDS             7261..>8492
                     /locus_tag="VGQ51_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV9036974.1"
                     /translation="MRDTAAEKQQPQQLRRVLGLWDLIFYGIVLIQPIAAIGLFGIAS
                     KVSRGHMVTTLLIAMLGMMLTAASYGRMASLYPSAGSAYTYVGKGLNTYLGFMAGWAM
                     FLDYLIVPVINTVYAALTLQRLMPSVPFVVWVILFVFVITYLNLRGIRTMARSNEILL
                     FVMCLVIGIFIWLGIRYVFHAQGWSGLFSYKPFYNPATFNIGAIMTATSFAALTYIGF
                     DGVTTLAEDVRNPKRNMLLAPILVCLFTGLFSGLQIYLAQRIWPDYTTFANPETAFFD
                     VSALVGGKFLFNAVAAILFIACLGSGLAGQVGAARLLFGMGRDGVIPGKIFSHLDARR
                     ATPTYNIVIMGVLTIAGSILLSYQAAAELLNFGAFLAFMGVNIATLRQFFFLRPPGER
                     RRFVPDFFFPVLGFLVCFV"
BASE COUNT         2050 a         1902 c         2474 g         2066 t
ORIGIN      
        1 gatggcaggc cgaggaatat catttttatc ctgacggacg atcatcgata tgacgcactg
       61 ggatttttga aaagccagcc gtttatcaag acacccaacc tggaccgcct ggcccaaggt
      121 ggtgcctatt taaaaaacgc cttcgtcacc acttccttat gttcgccttc gcgggcgtcc
      181 attctcaccg gcttgtatgc gcataaacat aaggtggtgg ataataacaa tccggttcca
      241 aaagacctgg tctttttccc gcagtatatg cagcaaatag gatatcagac ggcgctggtc
      301 ggcaaatggc atatgggtgg tgatttcgat gatccgcaac ggggattcga ttattgggtg
      361 tcttttaagg gccagggctc ctatctgccg gaaaagaacg ggttgaacgt aaacggaaaa
      421 cacgtgccac agaagggata tattaccgat gagctgaccg attatgccct ggacttctta
      481 aagagccggg ataggaataa gccgtttatg ctgtatctgt cacacaaggc agtgcatgcc
      541 gattttatac ccgcagatcg taacaagggc atgttcaaag gctacccgtt ccatccgcct
      601 ttgacgatgc aggcgggtac acaggagggc gccccgatgt gggttcaaaa tcagcgcaac
      661 tcctggcacg gagtagaatt cccttaccat agcagtctcg atatcgggga atattaccgg
      721 cgatatgccg agacgttgta tggcgtggat gaatcggtgg gccggatcat ggcgtacctg
      781 aaagagcagg gtttattaga atcgacactc atcatctata tgggtgataa cggttttcag
      841 ttcggggaac atggattgat cgacaagcga accgcgtatg aagcatccat gcgggtgccc
      901 atgctggcct ggtgtcccga attgatcaaa cccggcacgg tcgtaaaaga agtcgtcgcc
      961 aatatcgaca tcgcacccac gctgttggac gcggcgggtt tgcgggcgcc ggattatatg
     1021 gacgggaaga gttttctgcc gctgctcgaa gggaaaaagg aaccctggcg ggacgggctg
     1081 ctctatgaat attattggga gcgaaactat ccccagacgc cgacgatgca tgcgatccgc
     1141 ggtgaccggt ataagtatat ccattacaac ggcatctggg ataccgatga gctgtacgat
     1201 ctgcagaatg atccgctcga gaccaggaac cttatccggg ataaggcgtg ccaggacatc
     1261 gtaaagaaaa tgaatcacga gcttttccaa cagctggagg agtcgaaggg aatgtatata
     1321 ccgttgaatc cggatagggg agaacagtcg aatttgcgga ataggtatgg ttcgccggcg
     1381 gcggcattcc ccgattatct tttgcgcgga gacaccattt cggcattccc ggcgactata
     1441 cagctttcgc cggccgatag tggtaagcag cgtccggcgg ctatgcggga atctatagat
     1501 gatattataa aaaaggggtt cggccatttt tgggtgtatg gttcttccgc cgatgcgaat
     1561 aaggaggctt tggattatgc ggagtcccgg gggatgtatg tcgactatat gacctccggg
     1621 tttgaaggtt tcgatcgcga tcatcccccg gtgatgagtg tatacgaccc acagtatgcc
     1681 agcgaggtgc agaagcgggt ggcggccgga ctggctccca tgaaggcgtt gaaacgcgta
     1741 tatgccgtct tcccattcca ggatgaaccg tttcatgcgg gcgcttccgc attcgactac
     1801 agcagctttg cgagggctga atttgccaaa cgatatgggt acacgatgcc ggacagtctt
     1861 ggtgccgtta agaatgatcc gaagaagtgg gtggacctgc tcaacttcca gagcggaacc
     1921 ttcagcgatg gctggaagaa agtctatgat gccgtcaagg ccttcgatcc ccggccgggg
     1981 atcgtgatga cccatgacag ccacaataca tttggcgcgg gcgtaaagtc caactcgaag
     2041 atagccatgg atgacgtttt tcattggggg ggcgatttcg ccgatatcct ggcttatgac
     2101 atctatccgt atatgacctt cgattaccgg tacggagagc cgggtagact gcggaagccg
     2161 aggatcagtc aaatgcatta cacgatcgcc cagcttcgca atgtttgcac gacatacggt
     2221 aagcaaatgg gtttctgggt gggcacctat agtgacaatt ggtttcaacg gttcaagggt
     2281 cccgaaagga agagccagta ttggtcggag cacgaaatgg cctatactgc cattgcccag
     2341 ggcgccaatt tcctgatctc gccgtcgaat tacaatacga ccgatcttcc gctggattcc
     2401 atgcactggg cggattatgg gaaggcgatg agtgtaatcc agcaggcagg ccccgggttg
     2461 ttaaaggcgc cgcgtgtaaa ggccagggcc gcgtttcttt ttccgaggag ccagtatttg
     2521 ttgttgcggg aagaatactt caatgtcggg ttgacatatg aggcgttcct gcgggctttt
     2581 ggcgaattgg atatcctgca tgaggaccag atcacggatg ataagctcga tgggtaccgg
     2641 gtgttggtac ttgccgatgt caaattgctg cccgcgaagg tggccggtta tatagcatcg
     2701 tttgtccgga atggagggat agtcatcgcc gattgtgttc ctaatatgga tgaccgtaag
     2761 cggccgctcg aagggatggc gacactattc ggagtgagcc atgcggcgac ggaccgggtg
     2821 aaggaggagg gccagtgggt acccttctcg gcgattcctc cgaagatgtc gttcccgccg
     2881 gcgggcaagg catctgcggc cgatgaaaag gaagccagga cagatagggt agagggaacc
     2941 gcatttcagc agcattttgt ttttccagtc gtcagcccgc atggctgcga ggtaaagggt
     3001 ggtagggtcg ttttgaaaat gcggtcgggt cgccctgcgc tgatgtgcag gcaaaccggt
     3061 aagggaaaga cgtggctctt cggattcagt atgcaggata cctatttcaa tacctggaag
     3121 aacaaggaca gcgccgggcg gcaggagctg gaagatctca tcggtgatgt cttccgggag
     3181 tcgaagattc aagctcatat caactcgtcc aatccggaca tcgaggcttc ggtgcgggca
     3241 aatagcaacg aaggatatgt attcgtcatc aatcatgagg cggtgaatcc cggaacaacg
     3301 gttcggattg ccgacctgaa ttttaaattg gcaatatcat cgacataaag accggtaagc
     3361 ccgtcccgta cagggagaaa gaaggggttg tcgaatttag tataaacgca gcttttggcg
     3421 caacaaggct gttaaagctg ttacccgaat agtcacttta aaaaagtcat ttatgaaaaa
     3481 aattgttcat cctgaccggg atccgggctt caacacgggc gcttattcag atggagtaat
     3541 ggtagatgga tttctttttg tcagcggcca ggcggctgtt gacttcaaga cctcgaaatt
     3601 tatcctgggt tccatagaag aagagacaca tcggacgatg caaaatatcc gctcgatcgt
     3661 gcaggctgcg ggggcttcga tgggacaagt ggtcaaatgc acggtgcatc tcagcgatat
     3721 caaagatttc gaccggtata atgaagtgta tgcgacgtat tttccaggta taaaaccggc
     3781 gaggacgact gtacaatcgg tccttgccga agggataaaa gtggagatcg actgtattgt
     3841 gcgttgtacg ggtattgctt aatctattgt aaaatataca tgggaaagat tcagacagag
     3901 gatattgagg taggcgttgt cggactaggg ttaatgggta ccagttttat tgcttctttg
     3961 cttatcgcgg gtcacagggt caaggccgtc gccccgctcc cggatgatat gagggcggcg
     4021 ccgcagcgga taaagaatca tttaattgcc tgtgaaaggg ccgggctcct atgcaaaccg
     4081 atcgattcgt atttggctaa gctgttgatc gcagaagact acgagatgct aaagagctgt
     4141 cacctggtgg tggaatgcgt gatagagatt gccgggataa aacgttcgat ttacgaaaag
     4201 atcgagcggg tcgttgcaaa agatgccatc atcgccagca atacatccgc catcccgatc
     4261 agcgagttgc aaaaagacct tgtcaacccc gcgcgttttt tgggcattca ttgggcggag
     4321 ccttcgtatt ttacgcgctt catagaaatt acgaacgggg ctcagaccgc gatccattat
     4381 gccgaatggg tgtatgcgct ggcgcatttt tgggggaaag aacccacgct ccttcgcaag
     4441 gatgtaagag gatttatcac caaccggctg atgtatgcag cctatagaga agggttaagc
     4501 atcgtctcga agggggaagc gacactggaa gacgtggata aggcttttag atacgatgcg
     4561 ggttcctgga tgaccttgat gggcgttttt agaagaatgg attttttggg attgaaagat
     4621 tttctggaaa tattccggcg gacctttcct cagctctgca atgatgagga ggtccctgcg
     4681 ctgatgcaaa aaatggtcga tatcaatgca cggggagtcc agaatttatc ggggctttat
     4741 gcttattccg aggaagaggc ggcaaagtgg aatgaagcgt ttgccttgtt taatgtggat
     4801 atctttagat tggccgcatc gtaccctgcg acgcttatgg cacaaacagc cagtataact
     4861 aatttttaaa tcaaagtgca tatggaaaaa tacagctata ccgaatccgc ggccttgttg
     4921 gcaagagcga gaaaagccct ggcgggcggt gtttcttccg aattccgaaa gtacaatcac
     4981 ccccatgcca tattttataa ccatggggaa gggagttata tctatgatgt cgacggcaat
     5041 aagtatctgg attttacgct tagccagggg ccgttgatcc tggggcattc tcacccggag
     5101 gtattgaagg cggtggcgga atattcgcgt cagggacagc tttttgcagg gcagcatata
     5161 aaggaaatag aattggcgga aaaactgcaa gagctgatct cctcggcgga attgatacga
     5221 ttttgcctgg atgggtccac tgctgttcaa accgctttga gagtggcgag ggccaatacc
     5281 gggcgccaaa aattccttcg gttcgaggga cattaccacg gctggctgga caatgtgagc
     5341 tgggggatat ccgcttcttc cgccgaagcc atggggagca gggaacaacc aaaggtcttt
     5401 ccatggaccg aaggactgcc ggagagggcc agggatgaat ttatcatact gccatggaat
     5461 gatcttgagc tggtgaaaaa agttgtcgct gagaggcata aggaaatagc agccatcatc
     5521 acggagccgg tgatgtgcaa caatagctgt atactcccag cggagggatt tctcgaaggg
     5581 ctccgggaga tttgcgatga atatggcatc gcccttatat ttgacgaggt gatcacaggt
     5641 ttccggatgt ctcttggggg ggcacaacag tatttcaatg ttatccccga cctttccatc
     5701 tttgcaaagg ccatggggag cggttacccg atcagcgcgg tagtgggcaa gcggaaatgg
     5761 atgaacctca tccaggacgc cagggtgatc catgcgggta ctatgaatgc cggcaacccc
     5821 acggtcgccg cggcgctggc cactaccagt gtgttggagg ctgagcgacc ctatgagcgc
     5881 atgtttcttt acgggcagcg attgatggat ggtctgcggc aggcggctgc cgcggcgggg
     5941 caaaaattgc tggttcaggg cccggggccc atgttccata tgggatttac ccatttggat
     6001 aaggtgagcg attaccggga ctccttcaac tatgataagg ccaaactcgg gaaatttatc
     6061 gccggcatgc acgaccgggg tgtgcgggtg attggccgcg ggttgtggta tataagtgcc
     6121 gtgcataccg aagaggagat cgataccgcg ataaaggccg cttcggaagt aatgcaaacg
     6181 atataaaact aaaaagatga gcgacaggcg agggtttata aagaatgtgt cggggttagg
     6241 tctgctgggc gcggcgccgg ggcttttatc gccccgggcg aacagctttc gcccgcaggc
     6301 tggcgccggt gacggcaaga tatggggctg tctcctgcat ctgagctata acttttgggt
     6361 ggaatacagc actccgagcc catttagggg gtatcgtcct tatctgcaaa tgagcgaacc
     6421 cttatggaat gatgctacgg agaaaatggt tcaggaaggg ttgaacttag tggtgatcga
     6481 tctcggggat ggtgtcaggt acgaaagtca tcccgagatt gccgttaaca atgcatggtc
     6541 cacgacgcga ttgcggaaag aattggaacg tctgcgtaag atggggctgg aacccatccc
     6601 gaaattgaat tttgcagccg gtcatgacat ctggttgggg aaatattcac ggatggtatc
     6661 gacggacaaa tattatgaag tttgcggcga tcttatcgag gaggtgtccg gtctctttga
     6721 aaagccccgt ttttttcact tggggatgga tgaagaaaat gccgaggatc agcgatatct
     6781 cgattacgtc gtcatacggc aaaatgacct ttggtggcat gatctctatt tttttgtaga
     6841 ccgggtcgaa aaatgcggtt cgcggtcctg ggtctggtcc gactacttat ggcataaacc
     6901 cgaagcattc tttaaaaaga tgccgaagtc ggtgctgcag agcaattggt actacgacga
     6961 aaaattcgat gagaaattaa cgccggtaaa agcatatctc gatttggagg cggatgggta
     7021 tgaccaggtt ccgaccggtg gcttttacag aaaggacggg acaggcgaaa agagtatacg
     7081 caatacagta gaattttgcg ccgcgcatat tgccgatccc cggttatacg ggttcctgca
     7141 aacggtttgg gcgcccacca cggaagacaa cagagaaagt atcctgaaag cgatccgcct
     7201 ggctggggaa gcaaagaact ggttcgacaa agatcacaac aagccggcta aagcatgagc
     7261 atgagagata cagcagccga aaaacagcaa ccgcagcaat tgcgccgggt cctgggtttg
     7321 tgggacctca tcttctatgg cattgtgctg atacagccca ttgccgccat cggtttgttc
     7381 ggcatcgcct cgaaagtatc ccggggtcat atggtcacga cgcttttgat cgccatgctt
     7441 ggaatgatgc tgaccgcggc aagctatggt cgcatggcct cattatatcc cagcgcgggg
     7501 tcggcatata cctatgttgg gaaagggttg aatacctatc ttggttttat ggccggctgg
     7561 gcgatgttcc tggattattt gatcgtgcct gtcatcaata ctgtttatgc ggcgctgacc
     7621 ctgcaaaggc tgatgccatc ggttcctttt gtggtatggg tcatcttgtt cgttttcgtt
     7681 atcacgtatc tgaatctgcg ggggatccgc acgatggcga ggtcgaatga gatcctgctc
     7741 tttgtcatgt gtctcgtcat aggcatcttt atctggctgg gcattcgcta tgtctttcat
     7801 gcgcaggggt ggagcggcct tttctcctat aagccatttt ataatcccgc cactttcaac
     7861 atcggcgcga taatgacggc tacatcgttt gcggcgctaa cgtatatcgg attcgatggg
     7921 gtgaccacgc tggcagagga tgtcaggaac ccaaagagga atatgctcct ggcgccgata
     7981 ttggtatgtt tgtttaccgg gttattcagc ggtcttcaaa tttatctggc gcaacgcatc
     8041 tggccggact atactacatt cgccaacccc gaaaccgcat ttttcgatgt ttcggcgctg
     8101 gtgggaggaa aattcctttt taatgcggta gccgccatcc tgttcatcgc ttgtttgggg
     8161 agcggtctgg cgggacaggt tggagccgcc cgattgctct ttggaatggg aagagatggc
     8221 gtaatacccg gaaagatctt ttcccatctc gatgccaggc gagccacacc cacgtataat
     8281 attgttatta tgggtgtatt gacgatcgcc ggctcgatac tcctaagtta ccaggcggcg
     8341 gcggagttgt tgaattttgg cgctttttta gcttttatgg gtgtaaatat cgcgacgctc
     8401 cggcaattct tttttcttcg tccgccgggg gagaggcgaa gatttgttcc cgacttcttt
     8461 tttccggtac ttggattcct ggtgtgcttt gt
//
LOCUS       DASYSV010000339         6225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1730015, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000339 DASYSV010000000
VERSION     DASYSV010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730015"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VGQ51_10150"
     CDS             complement(<1..436)
                     /locus_tag="VGQ51_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEV9036975.1"
                     /translation="MQNVLKVHPADNVLVALTPLEKGSRISFDGEEYTIQEVIKAKHK
                     FATADLRPGDPVIMYGVLVGKAQSPIKKGGLISTSNVKHAANDFTVGDRKTEWARPDV
                     AGFTGRTFNGYHRADGSVGTANYWVVVPMVFCENRNLDVLQQA"
     gene            complement(438..1913)
                     /locus_tag="VGQ51_10155"
     CDS             complement(438..1913)
                     /locus_tag="VGQ51_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEV9036976.1"
                     /translation="MNLSKENLPAISNPAVIVPAEAIFSLPEKVLQFGTGVLLRGLPD
                     YFIDKANRHGMFNGRVVVIKSTDGGDAGAFDRQDGLYTLCVRGIEDGHKVEENIISSA
                     ISRVLSARDQWHTILQLAHNPELRIIVSNTTEVGIQLVEDDIRRSPPTSFPGKLLAFL
                     YERYQAFGGSPESGMVIVPTELIVDNGKKLAAIVKEMAQMAQLDASFLDWMEEHCRWC
                     SSLVDRIVPGRPDAAAQQQLAHDLGYTDDLLAISEVYRLWAIEGDEKVRSVLSFAAAD
                     PGVIIEPDIEIYRELKLRMLNGTHTLSCGLAYLSGFGTVKNAMDDSGFSAFVADLMLK
                     EIAPAIPYEVALAEARDFGLRVLDRFRNPQLQHQWLSITMQYSSKMRMRVVPVLLRHY
                     QRHSEPPANMALGFAAFLLFMRGGTHPVNDDRAGYFAQLWQQYPAPEVATVVLKDKSL
                     WGADLSQLPGFEGAVRLHLDNLIHKGAAATLVPLVKFKSIL"
     gene            complement(1983..2678)
                     /locus_tag="VGQ51_10160"
     CDS             complement(1983..2678)
                     /locus_tag="VGQ51_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV9036977.1"
                     /translation="MNDKKIILQSILGQGILPLFYWESPSVCLKVAQTLYRAGVRAIE
                     YTNRGTAALDNFIMLKKTLATEAPDLYLGIGTIKSAKEAGDFVRAGADFLVSPIVNPE
                     VAAVAESAGLLWIPGCMTPTEIYRAQELRADIVKIFPANILGHQFVSSIRELFQDQLF
                     IPTGGVEIDEDNIRSWVRAGVCAVGLGSRLISKEILLTENYDLLYKKTLEALKIARAV
                     LQARDAPGPKDNH"
     gene            complement(2668..3690)
                     /locus_tag="VGQ51_10165"
     CDS             complement(2668..3690)
                     /locus_tag="VGQ51_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV9036978.1"
                     /translation="MNKKVLSFGEILLRICPDAGGAWLRENELPFYVGGAEANVATAL
                     ALWGVPSAYCTALPDHLLSHQLVAYLKEKRIDTSRILFQGHRLGLYYLPKGKDLKNAG
                     VIYDREHSSFWCLQPGTVDWDTVLHDVGWLHFSAISPALNSRVARVCDELLEAAVGRN
                     ITISVDLNYRSKLWQYGCAPTDIMPGLVRHCDMVMGNLWAAEKMLGIPVDAALTEGPK
                     EDYLEHARLSSERIMHSFPRVRWVAKTFRFDHGTAGIRYYTTFYTEGKLYSSREYVSD
                     TILDKVGSGDCFMAGLIYGLWKKMPVQDLLEFATAAAYQKLFIPSDATDQRVEDIAQF
                     LTTYER"
     gene            complement(3687..4697)
                     /locus_tag="VGQ51_10170"
     CDS             complement(3687..4697)
                     /locus_tag="VGQ51_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV9036979.1"
                     /translation="MKTLVCIQPGEFAYREGDAPLARPGHSIIKIRRIGICGTDLHAY
                     EGTQPYFSYPRILGHELAGDLVDLDQAPGFQPGEAVTIIPYFSCGRCIACRMGKPNCC
                     TSIQVCGVHIDGGMVEYLSVPSSSLVAGRGLGYDQLALIEPLAIGAHAVRRADIRPGE
                     FVLVVGAGPIGLGTMESARIAGGVVIAMDINADRLRFCREKIQVLHAVNAADRPLEQI
                     EHITRGDMPTVVIDATGSRKAINDGFQYLAHGGRYVLVGLQKGEIQFSHPEFHKREAT
                     LMSSRNATREDFDNVMSAMEKGLIDPRTYITHRVIFPEVKTEFASWLDPSGGVIKAIV
                     EL"
     gene            complement(4702..5490)
                     /locus_tag="VGQ51_10175"
     CDS             complement(4702..5490)
                     /locus_tag="VGQ51_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV9036980.1"
                     /translation="MNLELKDKVIVVTGGAKGIGEGIVKVLAAEGAIPFIVGRNQADN
                     EATVSAVEAAGGKAYQTVAELTDPAACEAAVRAVVATCGRIDGVVNNAGVNDGVGLES
                     GNYEGFMASLHKNLVHYYLVVHYALPELKKTRGAIVNIGSKTAETGQGGTSAYAAANG
                     GRNALTREWAVELLKYGIRVNAIIVAECWTPLYERWIRTLPDPKTKLDEIVKKIPLEH
                     RMTTAEEIANMAAFLLSPRSSHTTGQLIHVDGGYVHLDRALANA"
     gene            complement(5487..>6225)
                     /locus_tag="VGQ51_10180"
     CDS             complement(5487..>6225)
                     /locus_tag="VGQ51_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9036981.1"
                     /translation="LPEHLHPLLQECGIDGCVLVQADQSERENEFLLGLAGSFDFIKG
                     VVGWVDLRDSGVEERLDYYKGFPKMKGFRHVLQGEPDRALMLAPSFKRGIGALASYGY
                     TYDILIYPDQLGYTREFVGCFPDQPFVIDHIAKPHIKDHFITEEWRSAIRSVATHPNV
                     CCKISGMVTEADWEHWKPEHLRVYIDTVIEAFGTDRILFGSDWPVCLVAASYKQVFDV
                     VKDYFSAFSADEQAAFFGGNAIKFYKL"
BASE COUNT         1398 a         1888 c         1569 g         1370 t
ORIGIN      
        1 atgcctgctg caagacatcg agattccggt tctcgcagaa caccatgggg accaccaccc
       61 agtaatttgc cgtaccgacg ctgccgtcgg cgcgatgata cccgttgaag gtgcggccgg
      121 tgaagcccgc aacatcgggt cgcgcccatt ccgttttccg atcgccgaca gtgaaatcat
      181 tggccgcatg tttcacattc gaagtactga taagaccgcc ttttttgatc gggctctggg
      241 cctttcccac caatactcca tacatgatga ccgggtcgcc gggccggaga tcggcggtgg
      301 cgaacttatg tttcgctttt ataacctcct gtatcgtata ttcctcgccg tcaaaagaga
      361 tccgcgatcc cttttccagc ggcgtcagcg ccaccagcac attgtcggcc ggatggactt
      421 tcagaacatt ttgcatacta taaaatgctt ttaaatttta ccagaggaac cagcgtcgcc
      481 gcagcccctt tatgaattaa attgtccagg tgcagtctca ccgcgccttc gaaaccgggc
      541 agctggctga gatcggcgcc ccacagcgat ttatccttca acacgacagt cgccacttcc
      601 ggcgccggat attgctgcca cagctgagca aaatatcccg cccgatcatc gttgacggga
      661 tgggtaccgc cccgcatgaa cagcagaaag gcggcaaaac ccaacgccat attcgccggc
      721 ggctccgaat gccgctgata gtgtctcagc agcaccggga cgacacgcat ccgcattttc
      781 gacgaatatt gcatcgtgat gctcaaccat tgatgctgca gctggggatt gcgaaacctg
      841 tccagcaccc ttaatccaaa atcccgcgct tcggccagcg ccacctcata cggtatcgca
      901 ggggctatct ccttcaacat caggtccgcc acaaacgccg aaaagccact atcgtccatc
      961 gcattcttca ccgtcccgaa acccgacaga tatgccagac cacagctcag cgtatgcgta
     1021 ccgttcagca tccgcagctt gagctcccgg tagatctcga tatccggctc gatgatgacg
     1081 cccggatccg ccgccgcaaa agacagcacc gaacgcactt tctcatcccc ttctatcgcc
     1141 catagacgat agacttccga gatcgccaac aggtcatctg tataaccaag atcatgtgcc
     1201 aattgttgct gcgcagcggc atcgggtctg cccggaacga tgcggtccac caggctgctg
     1261 caccaacggc aatgctcttc catccagtcg agaaaggatg catccagctg cgccatctga
     1321 gccatctctt tgacgatggc ggccagcttt ttcccgttgt ctacgatcag ttcggtagga
     1381 acgatcacca tgccgctctc ggggctgccc ccgaaggcct ggtacctttc gtacagaaaa
     1441 gccagcagct tgccgggaaa agacgtcgga ggactacgcc ggatatcgtc ttccaccagc
     1501 tggatgccca cttcggtagt gttggagacg atgatccgca gttccgggtt atgcgccagc
     1561 tgcaggatcg tgtgccactg atcacgcgcc gagagtaccc ggctgatggc gctgctgatg
     1621 atattttctt ccaccttatg cccgtcttcg atgccgcgga cgcaaagggt ataaagacca
     1681 tcctgccgat cgaaagcgcc ggcatcgccg ccatccgtcg atttgatcac gaccacgcgt
     1741 ccgttgaaca ttccgtgacg gttggctttg tcgatgaaat aatccggcaa cccgcgtagc
     1801 aggaccccgg tgccaaactg caaaactttt tccggaaggg aaaaaatggc ctcagccggc
     1861 acgatgacgg caggattcga aatggccggc agattttctt ttgataggtt catgctcatt
     1921 tattttcgtt agatgtgccc ggtctgtgct tcagcgaaaa atagcgcatc ccgggcaaag
     1981 cgctaatgat tgtccttcgg accaggcgcg tcccgcgcct gcaggactgc cctcgcaatc
     2041 ttcagggctt cgagtgtctt cttatacaac aggtcataat tctccgtgag aaggatctct
     2101 ttgctgatca gccggcttcc cagcccgacg gcgcatacgc ccgcacggac ccagctccgg
     2161 atattgtcct catcgatctc cactccgccg gtgggaatga acagctggtc ctggaacagc
     2221 tcgcggatcg agctcacgaa ttgatgtccg aggatattgg ccggaaagat cttgacgata
     2281 tccgcccgga gctcctgcgc gcgatagatc tcggtcggcg tcatacatcc cggtatccag
     2341 agcagacccg ctgattccgc gaccgccgct acttccgggt tgacgatggg tgacacgagg
     2401 aaatcggctc ccgcacggac aaaatccccg gcttctttcg ccgattttat ggtgccgatg
     2461 cccaggtaga gatcgggcgc ctcggtagcg agcgttttct tcaacattat gaaattgtcc
     2521 agtgctgccg ttccgcgatt ggtatactcg atggcccgga cacccgcgcg gtaaagggtc
     2581 tgcgccacct tcagacaaac cgaagggctt tcccaataga acaaaggcag gataccctgt
     2641 ccgagaatgg actgcaatat gatcttctta tcgttcatac gtcgtgagga attgcgcgat
     2701 gtcctccacc cgctggtcgg tggcatcgct gggaatgaaa agtttctgat aggcggccgc
     2761 ggtcgcgaat tcgagcaggt cctgcaccgg cattttcttc catagtccat agatcagccc
     2821 cgccatgaag caatctccgc tacccacttt gtcgaggatg gtgtcggaga catattcgcg
     2881 cgaagaatat agttttcctt cggtgtaaaa ggtcgtgtaa taccggatgc ccgccgtccc
     2941 atgatcgaac cggaaggtct tggcgaccca gcgtacccgg ggaaatgaat gcatgatccg
     3001 ttcgctgctg agacgggcat gttcgagata atcctccttc ggcccttctg tcagcgcagc
     3061 atctacaggt atacccagca tcttctcggc ggcccaaaga ttccccatga ccatatcgca
     3121 atggcgtacc agtcccggca tgatatcggt gggcgcgcag ccatattgcc aaagcttgct
     3181 ccggtagttg aggtccaccg aaatagtaat attccgcccg accgccgcct cgagcaattc
     3241 atcgcagacc cgggctaccc ggctattcag agccggcgag atcgcgctga aatgaagcca
     3301 gccgacatcg tggagcaccg tgtcccagtc gacggtgccg ggctgcagac accagaagga
     3361 cgaatgctcc cggtcataga taacaccggc attcttgaga tccttgccct tgggaagata
     3421 gtaaagccct aaacgatgtc cctggaaaag gatacgcgac gtgtcgattc ttttttcctt
     3481 caaataggcg accagctgat gcgagaggag atgatccggc aatgcggtac aataggcgga
     3541 aggcacgccc cataaggcca atgcggtcgc gacattcgct tcggcgccgc ctacataaaa
     3601 aggcagctca ttctccctga gccaggcgcc gccggcgtcc ggacagatgc ggagcagtat
     3661 ctcaccaaaa gacagtactt tcttgttcat aactcaacga ttgctttgat gacgccgccc
     3721 gacgggtcca gccaggaggc aaattccgtt ttaacttccg ggaagatcac ccgatgggtg
     3781 atatacgtcc tcggatcgat gagtcccttt tccatagcag acatgacatt gtcgaagtct
     3841 tcgcgggtcg cattgcggct actcatcagg gttgcttcgc gcttgtggaa ttcaggatgg
     3901 ctgaattgta tctcgccttt ctgtaacccc accaacacat agcgtccgcc atgagcaagg
     3961 tactgaaatc cgtcgttgat ggccttcctg ctgccggtgg cgtcgatgac cacggtaggc
     4021 atgtccccgc gggtaatatg ctctatttgt tccagcggtc tgtcggcggc attcaccgca
     4081 tgcaatacct gtatcttttc tcggcaaaac cgcagtctgt ccgcgttgat atccatcgct
     4141 atcacgacac ctccggcgat acgcgccgac tccattgtcc cgagtccgat agggccggcg
     4201 cctaccacga gcacgaattc accaggccgg atatcggccc gacgaacggc atgcgctcca
     4261 attgccaatg gctcgatcag cgccaattga tcgtaaccaa gtccccgtcc tgcgacaagc
     4321 gaagaggacg gcacggataa atattccacc attcctccat cgatatggac gccgcatacc
     4381 tgaatgctcg tacaacaatt gggcttaccc atcctgcatg caatgcaacg gccacaggag
     4441 aaatatggaa tgatagtcac agcttcaccg ggctgaaaac cgggtgcctg gtcgagatcc
     4501 accagatcgc cggccagctc atgccccagg atacggggat agctgaaata gggttgcgtg
     4561 ccttcataag catggagatc ggtaccacat atgccgatcc ggcggatctt aatgatggag
     4621 tggcccggac gggccagcgg agcgtcccct tcacgatagg cgaactctcc gggttgtata
     4681 caaacaagtg ttttcataag tttaggcatt cgccagggca cggtccaggt ggacgtatcc
     4741 tccgtctaca tgtatcaatt ggccggttgt atgactcgaa cgcggcgaca acaggaatgc
     4801 cgccatattg gcgatctcct cagccgtcgt catccggtgc tccaacggta tcttcttcac
     4861 gatctcatcc agcttcgtct tcggatcggg caatgtcctg atccagcgtt cgtacaatgg
     4921 cgtccagcat tccgctacga tgatcgcatt cacccttatc ccatatttca ataattcgac
     4981 cgcccattcc cgtgtcagcg cattccttcc accattggcc gcagcatacg ccgaggtgcc
     5041 gccctgcccc gtctcagccg ttttcgaacc gatattgacg atcgcgcccc tggtcttttt
     5101 caactcaggc aacgcataat ggaccaccag gtaataatgc accaggttct tatgaagaga
     5161 agccatgaaa ccctcataat tgcccgactc caatcctacc ccgtcgttga cgccggcatt
     5221 gttcactacc ccatcgatac gaccgcaggt ggcgaccaca gccctgactg ctgcttcgca
     5281 tgctgcggga tccgtcagct cggccaccgt ctgatacgct ttcccgcccg ccgcctctac
     5341 cgcggacacc gtcgcttcat tatcggcctg gttacgaccc acgatgaacg ggatcgctcc
     5401 ttccgccgcc agtaccttca cgatgccttc gccgatgcct ttcgcgccgc cggtgacgac
     5461 aatcacctta tcctttaatt ccaggttcat aatttataga attttattgc attgcctcca
     5521 aaaaaggctg cctgttcatc cgccgaaaag gcggaaaaat aatccttcac cacatcgaac
     5581 acctgcttat acgacgcagc caccaggcag acgggccagt cgcttccgaa aaggatccgg
     5641 tccgttccga atgcctcgat cacagtatcg atataaactc gaagatgctc gggtttccaa
     5701 tgctcccagt cggcctccgt gaccattccg gatattttgc agcagacatt cgggtgcgtc
     5761 gccacggacc ggattgcgga ccgccattcc tctgtgataa agtgatcttt gatatgcggc
     5821 ttggcgatat ggtcgatcac aaacggctga tccgggaaac atcccacaaa ttcacgggta
     5881 tagccaagct gatcgggata gatcaggata tcataggtgt aaccgtacga agccaacgcg
     5941 cctataccac gtttgaaaga aggagccagc atcagggccc tgtccggttc gccctgcagg
     6001 acgtgccgga agcccttcat tttaggaaaa cccttataat agtcgagacg ctcctccacc
     6061 ccactatctc gcagatcgac ccagccgacc acacctttta taaaatcgaa agatccggca
     6121 agccccagca ggaattcatt ctcacgttcg gattgatccg cctgtacgag aacacagccg
     6181 tcgatgccgc attcctgtaa aagaggatgc agatgctcgg gcagg
//
LOCUS       DASYSV010000340         6700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2497517, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000340 DASYSV010000000
VERSION     DASYSV010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497517"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3576
                     /locus_tag="VGQ51_10185"
     CDS             <1..3576
                     /locus_tag="VGQ51_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEV9036982.1"
                     /translation="EHLSGELPRAMIPSYFMRLPALPVTPGGKLDRQALPAPGLKDRQ
                     HYQAPSNEWEEKLVAIWATILRLDKETISVTDSFFELGGHSLRAMTLVNKIQGELGVE
                     ITIGDVFDARNIVNLGQLLQTSAKSDYMGLKKAERREYYPLSTAQRRLYYLYELDRTT
                     LTYNLPAVIRLEGPLDKEKLRRVMLELVRRHGSLQTSFEIVRDEPVQKISDIEDFGIE
                     YFRSAPDAIDGVIRQFIRPFGLHRAPLFRAGLIETAPEVHYLMADMHHIVSDGTSIGL
                     LINDFMALYNGATPPPLAFEYKDYAVWQQSAAYQRRIARQKEFWVNEFSEAISPLDIP
                     VDFVRPATTEGDFLWFDLTPEETRRLRALADAEVATISMVILAVLKVLFSRMARQDDI
                     VVGMAVAGREQYELEGLIGMFPVVLPLRTYPKTHMRFREFLSALKSAFLGVFDNQSYQ
                     YEELGRELKLERTSSRNPWFDVLYLYQNFETSELSLPDLKVSEYREQHIVAYEKLNLT
                     VKENDEQIFCKLIYSKALFREETMERLVKYFRQVVAAVIRDPDVVLSGIQLMNADERQ
                     ALLNQSGKISPLPRDKTFADLFEQQVKRTPKNPAVEHNGLILTYEELYAHSVKLASHL
                     VRAGAGPGRNVCVFLPRGIELLVSMLAIFRTGAVYVPADVDFPSQRVKEIVADSASGI
                     VITSLETVRMMNELKQVVPTIKEVICLDRLEDIRQANNLQMPRNADDLAYIIYTSGTT
                     GKPKGVMIHQLGLINHLYGMIDILQIDGRDGIAQTASPCFDISVWQFLTALLTGGRTI
                     IVDKDRVGDPSQMTDILQRCRITIFQTVPSVMAAFLEELPSGTDRSLPELRWMIPTGE
                     TLSASLVKKWYAIYPGIRLLNAYGPAEASDDIATFIVPPMEEGDHVVPVGKPVQNMRI
                     YILDESLNLCAIGIKGEICTAGPGVGKGYWKDAEKTRKAFVPNPYLREGDGADYAVLY
                     RSGDIGYYQPDGGIVFTGRQDEQVKIRGHRIELGEIESRLLGHENIREAKVLVKEGPG
                     GQALVAYYVSGEMIVAGELEAYLAARLPHYMIPSYFIHLRAFPLTVNGKVDKRALPDI
                     GIRTEDEYIAPRNSIDKKLAGIWAGLLQIPAETISMNANFFYVGGHSLNAITLLNRVC
                     KEFHIHMPLKEFLQKATIRWQSDTIHSQLGQAYSEPMSGTTEILI"
     gene            3582..5129
                     /locus_tag="VGQ51_10190"
     CDS             3582..5129
                     /locus_tag="VGQ51_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV9036983.1"
                     /translation="MNDVLNFFRAFAPREAGTEPGIEAGSRHRFTCADISLDQVRARF
                     MNSVLPDDPVSLERYYDWLNEDVLPYTIDTAAPTFIGHMTSALPEYVHDLSRLVSRLN
                     QNLVKIETSKSVTFLERECIAMLHRLFYGLPEDFYQEHIQKLNSNTGIIASGGSTANL
                     SALLSARNRLLYGPADEPAGSKSIYFLLREQGYRDMVVLGTELMHYSFRKTLSVIGLG
                     SENIVRVANDALGRMIPGDLEEKIRSCRKEGMLIVAVIGVAGSTERGSIDPLLQIGSI
                     ARQHHIYFHVDAAWGGALAFSEKHRMLLEGIEEADSITFCGHKQLYLPQGISICLFKD
                     PAQLGYNAITANYQAKADSFDTGRFTVEGSRPALSICLHASLCVIGKRGYGLLIDRSI
                     DHAVCFARMIEETGGFELISRQINIINYRYIPLPFRERWRNRELTVEINRQIDALNSE
                     IQNRQFHKGMTFVSKTTIGHAIYGRIVVLRAVLSNPLTTQEHLRQVLKDQFSIVDELI
                     ENQVYEP"
     gene            5119..>6700
                     /locus_tag="VGQ51_10195"
     CDS             5119..>6700
                     /locus_tag="VGQ51_10195"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV9036984.1"
                     /translation="MNHKSLLTLMALLAGSAVFPRCADAQQPYFISDGRKVLIDTFNT
                     GIQRMMDDIGIPGMSLAVIEDNKVVYHRNYGYKNIDTKEPVDQSTIFEAASLSKSYLV
                     YSVFKLVEKGILDLDKPMYQYMPYPLLEHDQRYKRITPRMILCHSSGIENWMEYNIPD
                     TLEIESDPGTKYVYSGEAYNYLSKVVALLLHESYEEYTKEIVLDPLGLKNSSLKFREM
                     PAGSPHPYEPSDYALGHDILEKQTGKWFNTDAVPSSANNVTAGDYAKLIVAIFDHRHL
                     SDKSIRTILGGRTVTRLNDDGKAYYFGCGFEVIFTGGDTIISHGGSNPGFKNMIFYSV
                     KSKRGFVYLTNSDRGRLVASRLSAMTAGLYLHQYFGDFGDEQYPSLSNDLLSVYRKKD
                     TLAMYAAIEKCRKEGMLTAKVMEDLGEELIHYNLPAGERVLEEGQQLFPESSRVYWLL
                     GLSHMYMDDFDNAYAYYLKARDLKFNAAQIADDLKKSAVRMNEAAKRKALITRIGPDR
                     KDTLQAEDYYAQHGIVQQA"
BASE COUNT         1622 a         1691 c         1943 g         1444 t
ORIGIN      
        1 gaacatttgt cgggagagct gccgcgggcg atgatacctt cctatttcat gcgtctgccg
       61 gcattgccgg ttacgccggg aggaaagctg gatcgccagg cgctgcccgc accgggtctg
      121 aaagatcggc aacattacca ggctccgtcc aatgaatggg aggaaaagct ggtagccata
      181 tgggccacga tcctcaggct cgacaaagaa accatcagcg taacggatag ctttttcgag
      241 ctgggcggac attcgttgag ggcgatgacg ctcgtaaata agatacaggg ggaattgggg
      301 gttgaaataa ccatcgggga tgtatttgac gcacggaata tcgtgaatct cgggcaactg
      361 ttgcagactt cggcaaaatc cgattacatg gggctgaaaa aggctgaacg aagagaatat
      421 tatccactgt cgacggcgca acggaggttg tattatctct atgagctgga ccggaccacg
      481 ctcacctaca atcttcctgc cgtgatacgg ctggaaggac cgctggacaa agaaaagcta
      541 cggagggtga tgctggagct ggtgcgccgt cacggatctt tgcagacatc gttcgagatc
      601 gtcagggatg agcctgtcca aaagatctcc gatatagagg atttcgggat cgaatatttc
      661 cggagcgcgc cggatgctat cgacggcgtg atccggcagt ttatcaggcc cttcggcctg
      721 catcgggcgc ctttattccg tgccgggcta atcgagaccg cgcccgaggt gcattacctg
      781 atggccgaca tgcatcacat cgtgtcggac ggcacttcca tcggtctgct gatcaatgat
      841 tttatggccc tttataatgg ggcgacacct ccgccgctgg cgttcgaata caaggattat
      901 gcggtgtggc agcagagcgc cgcttatcag cggaggatag cgcggcaaaa ggagttttgg
      961 gtgaacgagt tctcggaggc tatcagcccg ctggatatac ccgtggattt tgtgcgaccg
     1021 gccaccacgg agggcgactt cctctggttc gacctgaccc ctgaggagac cagacggctg
     1081 agggcgctgg ccgatgccga agtcgccacc atttcgatgg tcatcctcgc cgtgctcaag
     1141 gtgcttttca gcaggatggc ccggcaagac gacattgtcg tgggtatggc agtcgcgggg
     1201 agggagcaat atgagctgga aggcctgata ggcatgttcc cggtggtgtt gcccttgcgc
     1261 acctatccga agacccatat gcgattcagg gagttcctgt ctgcgctgaa gtcggctttc
     1321 ctcggagtgt tcgacaatca aagttatcaa tatgaggagc tgggaaggga gctgaaactg
     1381 gaacggacca gtagccgcaa tccgtggttc gatgtcctct atctctacca gaatttcgag
     1441 acctccgagc tttcgttgcc ggatctgaag gtctccgaat accgggagca gcatattgta
     1501 gcttatgaaa aactaaacct cacggtaaaa gagaacgacg agcagatatt ctgcaagctt
     1561 atctattcaa aagccttgtt cagggaggag acgatggagc ggctggtgaa atactttcgc
     1621 caggtggttg ctgcagtgat ccgggatccg gacgtcgtcc tttctgggat acagctcatg
     1681 aatgccgatg aacgccaggc gttgttgaac cagtcgggaa agatcagccc cctgccgagg
     1741 gataagacat ttgccgacct cttcgaacag caggtgaaac gcacccccaa gaacccggcc
     1801 gtagagcata atggcctgat attgacatat gaagagctgt atgcgcattc cgtgaagctg
     1861 gcatcgcatc ttgtccgcgc cggggcgggg cccggacgga acgtttgtgt attccttccg
     1921 cgcgggatcg agctgctggt cagcatgctg gccatctttc ggacaggagc cgtctacgta
     1981 cccgcagatg tggatttccc atcccaacgg gtaaaggaga tagtggcgga tagcgcatcg
     2041 ggtatcgtga tcacctcgtt ggagacggtc cggatgatga atgagctaaa gcaggtggtt
     2101 cctacgataa aagaagtgat ctgtctcgat cgcctcgagg atatacgaca ggcgaacaac
     2161 ctccagatgc cgcgaaacgc cgacgatctg gcgtacatta tctatacctc gggtacgacc
     2221 ggcaagccga agggagtcat gatccatcaa ctgggactga tcaaccacct gtatggcatg
     2281 atcgatattc tgcaaatcga tggccgggac ggcatcgcgc aaacggcatc accctgtttc
     2341 gatatttcgg tgtggcaatt ccttacggca ttgttgactg gagggaggac catcattgtc
     2401 gacaaagata gagtgggtga tccgtcgcag atgaccgata tcctgcagcg gtgccgcata
     2461 accatcttcc agaccgtgcc ttcggtgatg gcagcttttt tggaagagct gccttccgga
     2521 acggatcggt cattgccgga gctgagatgg atgataccga ccggggaaac gttgagcgca
     2581 tcgctggtga agaaatggta tgctatttat ccgggcatcc ggctgctgaa cgcctatggc
     2641 ccggcggagg cctccgatga tattgccacc ttcatcgtgc cgcccatgga agaaggcgat
     2701 catgtggtac cggtcggcaa gccggtacag aacatgcgta tctatatcct ggacgaatcg
     2761 ctcaacttat gtgccattgg aataaaaggg gagatctgca cggcgggccc gggggtaggc
     2821 aaaggatact ggaaggatgc ggaaaagacc cggaaagcat ttgtgcccaa tccttatctg
     2881 cgtgaagggg acggtgcgga ctacgccgtt ctctatcgca gcggcgacat cggatattat
     2941 cagccggatg gcggtatcgt atttaccggc cggcaggacg agcaggtgaa gataaggggc
     3001 cacaggatcg aactcggtga gatcgagagc cggttgctgg gacacgagaa catacgggaa
     3061 gcgaaggtgc tggtaaagga agggccgggc ggccaggcgc tggtggctta ttatgtatcc
     3121 ggggaaatga tcgtggccgg cgaactggaa gcctacctgg ccgcccggtt gccccattat
     3181 atgatccctt cttattttat ccatctgcgg gcattcccgc tcacggtcaa tggaaaggtg
     3241 gataagcggg cattgcctga catcgggatc cgaacggagg acgaatacat cgctccccgc
     3301 aattccatag ataaaaagct ggccggcatc tgggccgggt tgctgcaaat acccgcggaa
     3361 actatcagta tgaacgcgaa tttcttttac gtgggcggac attcgctgaa cgcgatcacg
     3421 ctgctcaaca gggtctgcaa agagtttcac atccatatgc ccctgaagga atttctgcaa
     3481 aaagccacga tacgctggca gtccgatacg atccattcgc agttgggaca ggcatacagc
     3541 gaaccaatgt cgggtacaac tgaaatcttg atctaacgac catgaatgat gtactgaact
     3601 ttttcagggc cttcgccccc cgtgaggcgg gaaccgagcc ggggatagag gcagggagcc
     3661 gtcaccggtt cacctgcgcc gatatatcgt tggaccaggt cagggcacgg ttcatgaaca
     3721 gcgtccttcc cgacgacccg gtatcgctgg agcggtatta cgactggctg aatgaggacg
     3781 tgctccccta tacgatcgat acggctgcac ctaccttcat cggtcacatg acctcggcgc
     3841 tgcccgagta tgtgcatgat ctgagcaggc tggtcagccg gctgaaccaa aacctcgtaa
     3901 agatcgagac atccaaatcc gtcacgttcc tggaacggga gtgtattgcg atgctgcacc
     3961 ggcttttcta tggtctcccg gaggactttt accaggagca tatccaaaag ctgaattcca
     4021 ataccggtat cattgccagc ggcggaagca cggcaaacct gtcggcactg ttatctgccc
     4081 gtaaccgact gctgtatgga ccggcggatg agcccgccgg cagcaaaagc atctatttcc
     4141 ttcttcgcga gcagggatac agggacatgg tggtattggg gaccgagctc atgcattatt
     4201 cttttcgtaa gaccctctcg gtaataggcc tgggttcgga gaatatcgtc cgggttgcga
     4261 atgatgcgct gggcaggatg atacccggcg acctggaaga aaagatacgg tcctgccgga
     4321 aagagggaat gctgatcgtc gccgttatcg gagtagcggg ctccacggaa agagggagca
     4381 tcgacccctt attgcaaata gggagcatag cccgacagca tcacatctat tttcatgtgg
     4441 atgcggcctg gggcggcgca ttggcttttt ccgaaaagca caggatgctg ctggagggta
     4501 tcgaagaggc cgattcgatc actttctgcg gacataaaca attatatctt ccccagggca
     4561 tcagcatatg tctgttcaag gaccccgcgc agctcggcta caatgcgatc accgccaatt
     4621 atcaggctaa ggccgacagc ttcgacacgg gccggttcac cgtcgaaggg tcacgtcctg
     4681 ccttgtcgat atgtctccat gcctcactat gcgtcatcgg gaaaagggga tatggactgc
     4741 tgatcgaccg cagcatcgat catgccgtat gttttgcccg gatgatcgag gagaccggcg
     4801 gattcgagct catcagcaga cagatcaaca tcatcaacta ccggtatatc cccttgccct
     4861 tccgcgaacg ctggcggaat cgggagctaa cggtagagat aaaccggcag atcgacgccc
     4921 tcaacagcga gatccagaac cgccagttcc ataaaggaat gactttcgtg tcgaagacca
     4981 ccatcgggca tgccatatat ggcaggatcg tcgttctccg ggcagtgctc tccaatcccc
     5041 tgactacaca ggaacatctc cggcaggtgt tgaaggatca attttccata gtagacgagc
     5101 tcatcgaaaa ccaagtgtat gaaccataaa tctcttttaa cgttgatggc gctcctcgcg
     5161 ggaagcgctg tcttcccccg gtgtgcagat gctcagcagc cctattttat atccgacgga
     5221 agaaaagtgc tgatcgacac attcaatacc ggcatacagc ggatgatgga cgacatcggc
     5281 ataccgggca tgtcgctggc cgtcatcgag gacaataagg tggtgtacca taggaactat
     5341 ggatacaaaa atatcgatac taaagaaccg gtggaccagt ccaccatctt cgaggcggca
     5401 tcgctttcaa aaagctacct ggtgtattcc gtattcaagc tggttgaaaa ggggatattg
     5461 gacctggaca agccgatgta tcaatatatg ccctatccgc tgctggagca tgatcagcgg
     5521 tataagcgga tcacaccgcg aatgatcctc tgtcattcgt cggggatcga gaactggatg
     5581 gaatataata tcccggatac cctggagatc gaaagcgacc cggggaccaa atatgtttat
     5641 tccggcgaag cctacaacta cctgtccaaa gtcgtcgcgc tgctgctgca tgagtcgtat
     5701 gaggagtata cgaaggagat cgtgctcgat ccgctggggt tgaaaaactc ttccctgaag
     5761 ttcagagaga tgccggccgg atcgccccat ccgtacgagc cctcggatta cgccctgggg
     5821 catgacatcc tggaaaaaca aacgggtaaa tggttcaata ccgatgccgt gccgtccagt
     5881 gccaataatg tcacagccgg ggattatgcg aagttgatcg ttgccatctt cgatcaccgg
     5941 catttgtccg acaaaagtat ccggacgata ttaggcggca ggacggtgac ccgtctgaat
     6001 gacgacggta aagcttatta tttcggctgc ggcttcgagg tgatcttcac gggcggcgac
     6061 acgatcatca gtcatggcgg cagtaatccc ggcttcaaga acatgatctt ttattcggtg
     6121 aagagcaaaa ggggtttcgt ctatctcacc aacagcgaca gaggacggct ggtggcgtcc
     6181 aggttgtcgg ctatgacagc gggtctgtac ctgcaccaat attttgggga tttcggcgat
     6241 gagcagtatc ccagtctttc caacgacctg ctttccgtat acagaaaaaa agatacgctg
     6301 gcaatgtatg ccgccatcga aaaatgccgg aaagaaggga tgttgaccgc aaaggtgatg
     6361 gaggatctgg gcgaagagct catacactat aacctgccgg cgggtgagcg ggtgctggaa
     6421 gaggggcagc agcttttccc ggagtccagt cgcgtttact ggctgttggg gttgtcccac
     6481 atgtatatgg atgatttcga caacgcctat gcctattatc tgaaggcccg ggatctgaaa
     6541 ttcaatgcgg cgcagatagc cgacgacctg aaaaagagtg cggtcaggat gaacgaggcc
     6601 gcaaaacgaa aggcgctgat aaccaggata gggccggata gaaaggatac gctgcaggca
     6661 gaagattatt atgcccaaca tgggatcgta caacaggcta
//
LOCUS       DASYSV010000341         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_191521, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000341 DASYSV010000000
VERSION     DASYSV010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191521"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VGQ51_10200"
     CDS             <1..834
                     /locus_tag="VGQ51_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9036985.1"
                     /translation="EIEKFLDTPVKRYSSGMYVRLAFAVAAHLEPEILIVDEVLAVGD
                     AQFQKKCLGKMEDVSKNQGRTVLFVSHNMPTVLNLCDKCIYLEKGEIRRMGDTSDIVS
                     EYIRQEGQVNVRWQPTNDKEGKGAAPYFTVTDFYLSSPSGKDCAVLEAEDNDKIDIVL
                     EGEVDELDVRLNMGFVLKNEYGDDIIMSYSTDQPEKDWVRYEKGKVRMKTTIDTSILN
                     EGKYYVYLLSSIHCVRMLLTPNDNICLGFEIIGNRGHSPYWFQRRPVILAPFIPWNNI
                     Q"
     gene            848..1465
                     /locus_tag="VGQ51_10205"
     CDS             848..1465
                     /locus_tag="VGQ51_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036986.1"
                     /translation="MNLLKRIVRRLRRMFTKKVKIPSYHVKRAILLGYKDRFRIGTMV
                     ETGTFLGDTVEFFRHKMDMVYSIELSEELAAKAVKRFEGISNVRIIQGDSGVILADLV
                     KQVPSTALFWLDGHYSSEFYVNDEFIRTARTDKDTPIMKELDVLLNDARQHVILIDDA
                     RCFNGQGDYPTVDTIRGLVKQSKFPYDVFIQQDIINIVPAEESKQ"
     gene            1484..2440
                     /locus_tag="VGQ51_10210"
     CDS             1484..2440
                     /locus_tag="VGQ51_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HEV9036987.1"
                     /translation="MKKTIRINFVDFWNGFIKEDNYFIHLLSRVYNVELSDDPDFIIY
                     SCYGREYLKYKCIRIFYTAENQRPDFSGCDYALSFDLQTGPRQYRLPLYALYIGLWGQ
                     EKNVVQPHTRETMAGEWQKKSKFCCMLVSNGGSKRRIDFFHTLSARKHVDSGGRYLNN
                     IGGPVKNKLEFIRDYKFVFAFENTSYPGYVTEKLIEPCFTHSIPIYWGNPQVALDFNP
                     KRFINCHDFPNDDAVIDKILEIDRNDELALDMLSQPIFKDNRYPEWIQEENVLTFLRS
                     IFDREPAIKPVALTGKRLVHELHWRSKLFGEKLKYRLHFHSH"
     gene            2443..3054
                     /locus_tag="VGQ51_10215"
     CDS             2443..3054
                     /locus_tag="VGQ51_10215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV9036988.1"
                     /translation="MAILRTISRKLRSLAGGGAGPQYRSLLKQGVLRTGNGCDLHSMQ
                     VILVGVTPGTLVIEMGNNCCVRGTIMVYRPTSKVVLGDNVYIGPGSWIECSVGVTIGS
                     NVLISMNCHIIDTNSHSLNSAHRLEDTIEWQKGLSYKDWSKVQCAPVSIGDKCWVGLR
                     SIILKGVSLGEGSIVAAGSVVTNAVQPYTIVGGNPASFIKATE"
     gene            3055..>3328
                     /locus_tag="VGQ51_10220"
     CDS             3055..>3328
                     /locus_tag="VGQ51_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9036989.1"
                     /translation="MGKIVFFLPDVVGGVASVVNNILQYAPDGSNVDVIAYSDSRISR
                     KTLDPMLYAGSFSRFIYNGDDSNFFTAKRLFRQTPSDLACIVATDVP"
BASE COUNT          839 a          865 c          846 g          778 t
ORIGIN      
        1 gagatagaga aattcctgga tacacccgta aaaagataca gcagcggcat gtatgttcgt
       61 cttgccttcg cagttgccgc tcacctcgag ccggaaatac tcatcgtgga cgaagtgctc
      121 gcggtaggcg atgcccaatt ccagaagaaa tgcctgggaa aaatggaaga cgtcagcaag
      181 aaccagggcc ggaccgtcct gttcgtcagc cataacatgc cgaccgttct gaatctttgc
      241 gataaatgta tctacctgga aaaaggcgag atccggagaa tgggggatac cagcgacatc
      301 gtttccgagt acatccgtca ggaaggacag gtgaacgttc gttggcagcc gacgaatgac
      361 aaggaaggca aaggcgccgc accctatttc accgttacgg atttttacct gagctcgccg
      421 tcgggaaaag actgcgccgt tctggaggca gaagacaacg acaagatcga tattgtcctg
      481 gaaggagagg tggatgagct cgatgtccgt ctcaacatgg gttttgtctt aaagaacgaa
      541 tacggtgacg atatcatcat gtcctattca accgaccaac ccgaaaaaga ttgggtccgg
      601 tatgagaaag gaaaggtcag gatgaagaca acgatcgata cctcgatact gaacgaaggc
      661 aaatactatg tttatctgct ctccagtatc cattgcgtca ggatgctgct gacgcccaat
      721 gacaatattt gtctgggttt cgagatcatc ggcaaccggg gacactcgcc ttactggttc
      781 caacgtagac ccgtgatcct ggcgccattc attccctgga acaatatcca ataaatactc
      841 aataccaatg aacttactga aaaggatcgt ccgccgtttg cgccgcatgt tcacaaaaaa
      901 agtgaagatc ccttcctatc atgtgaaacg cgccattctt ttgggttaca aagaccggtt
      961 ccggatcggt acgatggtgg agaccggcac ttttttgggc gataccgttg aattcttccg
     1021 gcataagatg gatatggtct attccatcga gctgagcgag gagcttgccg ccaaggcggt
     1081 caaaagattc gaagggatat ccaatgtccg gatcatccag ggagacagcg gagtcatcct
     1141 tgccgatctt gtgaaacaag taccttccac cgcccttttc tggctggacg gtcactactc
     1201 ttccgagttc tatgtgaatg acgagttcat ccgcacggcc aggacggaca aggatactcc
     1261 catcatgaaa gagctcgatg tcctcctcaa cgatgcccgc cagcatgtca tcctcatcga
     1321 cgacgccagg tgtttcaacg gtcagggcga ttatcccacg gtcgatacca tccgcggtct
     1381 ggtgaaacaa tcgaaattcc cttatgacgt ctttatccag caggatatca tcaatatcgt
     1441 tccggctgag gagagtaagc aataagaacc ttcactggcg tgtatgaaaa agacgatccg
     1501 gatcaacttc gtggatttct ggaatggatt catcaaagag gataactatt tcattcatct
     1561 cctttcgcgg gtatataatg tcgagctcag cgacgacccg gacttcatca tctattcctg
     1621 ctatggccgg gaatacctga aatataaatg catccggatc ttttataccg ccgagaacca
     1681 gcgccccgat ttttccggat gtgactacgc gctttcgttc gatcttcaga ccggtccgcg
     1741 gcaataccgc ctgccattat atgccctcta tatcgggctc tggggacagg aaaagaacgt
     1801 ggttcagccg cacacccgcg agacgatggc cggggaatgg caaaagaaat cgaagttctg
     1861 ctgtatgctc gtttcaaatg gcggatcgaa aagacgtatc gatttctttc acacattgtc
     1921 ggcgcgcaag cacgtggatt cgggcggaag atacctgaac aatatcggcg gccccgttaa
     1981 gaacaagctg gaattcatca gggactacaa attcgttttt gctttcgaga atacttccta
     2041 ccccggctat gtgacggaaa agctgatcga accttgtttt acccattcca ttcccatcta
     2101 ttggggaaac ccgcaggtgg ccctggattt caatcccaaa cggttcatca attgtcacga
     2161 ttttcccaat gacgatgccg tcatcgataa gatattggag atcgaccgga atgacgagct
     2221 ggccctggat atgctctcac aaccaatctt caaggacaac cggtaccccg aatggataca
     2281 ggaggaaaac gtgctgactt ttctccgatc gatcttcgac agggagccgg ccatcaaacc
     2341 ggtggcattg acgggcaaac ggctcgtaca tgagcttcac tggcgcagca agctcttcgg
     2401 ggaaaagctg aaatatcgtc tgcatttcca ttctcattga tcatggcaat tctccgcacc
     2461 atatcccgca aattgcgatc actggccggc ggcggggccg gcccgcaata ccggtcgctc
     2521 ctcaaacaag gcgttctccg tacgggcaac ggctgcgatc tccactccat gcaggtgatc
     2581 ctcgtcggtg tgactcccgg gacgctggtc atcgagatgg gaaataattg ttgcgttcgc
     2641 gggacgatca tggtttaccg gcccacttcg aaggtcgtac tgggcgacaa cgtttatatt
     2701 ggcccgggca gctggatcga atgctcggtt ggcgtcacca tcggtagtaa cgtcctgatc
     2761 tccatgaact gtcatatcat cgataccaat tcgcattcgc tgaattctgc gcaccggctc
     2821 gaagatacca tcgaatggca gaaagggctt tcctataaag actggagtaa ggtccagtgt
     2881 gccccggtga gcatcggcga taaatgctgg gtaggtctca gaagtatcat tttgaaggga
     2941 gtatcgctgg gagaaggctc catcgtggcg gccggcagtg tggtgaccaa cgcggtgcaa
     3001 ccctatacca tcgtcggggg caacccggcg tcatttataa aagcaacgga ataagtgggc
     3061 aagatcgttt ttttcttacc ggacgtggtc ggcggcgtcg ccagcgtggt gaacaatatc
     3121 cttcagtatg cacccgatgg cagcaatgtc gacgttatcg cctactccga cagccgcata
     3181 tctcgcaaga cactggaccc gatgctatat gccggtagct tctcccgctt tatttataac
     3241 ggcgacgata gtaatttttt taccgccaaa cgcctcttta gacagacccc atccgatttg
     3301 gcctgcatcg ttgccaccga tgtccccg
//
LOCUS       DASYSV010000342         7023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1347308, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000342 DASYSV010000000
VERSION     DASYSV010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347308"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VGQ51_10225"
     CDS             <1..634
                     /locus_tag="VGQ51_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV9036990.1"
                     /translation="PSDYQGWAYGLKKAGYATNVRYPQILIKLIKGYNLQQYTLIAMG
                     KMKPEEEVVLNMPGLPPAGQVVVEGKVDSGVADRPAVTDPAAAAAYPVGEFTINKTRV
                     VYVKAGVSLLAVAGQYDVPLPRLLEFNDMKEEDVLVKGQLVYLQRKRRTGANEYHVMR
                     DGESLYDVSQAEGIRLEDMLEMNQLTPGERPAPGERIYLQGSAPSRPRLL"
     gene            636..1181
                     /gene="hpt"
                     /locus_tag="VGQ51_10230"
     CDS             636..1181
                     /gene="hpt"
                     /locus_tag="VGQ51_10230"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV9036991.1"
                     /translation="MSVLKVHDKEFKPYLGAELIDEKIAAIATRIDEDYAGKRPLFIA
                     ILNGAFMFASDLFKKISIESEISFIKLASYKGTKSSGQVITAIGLDTDLHGRHVVIVE
                     DIVDTGKTLSEFLPQLEHQQPASLRIAALLQKPEATVYPIRVDYLGFSVPNKFLLGYG
                     LDYDGLGRNLPAIYQLVEGSE"
     gene            1232..2995
                     /locus_tag="VGQ51_10235"
     CDS             1232..2995
                     /locus_tag="VGQ51_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEV9036992.1"
                     /translation="MKPALLLLVGSLFFYTSRAQFYLRGEVKDENNSLLPDVRILLHS
                     SGYLYHSGSSGAFGIPIPSSADTVTLSADGFQTLVMPVDANRYQFIKMRPVGGAAPSA
                     PMRRGLLSLTKDVKAADRKDWTVGSETYSSLAENNFIPALRFPETGFAVTIDKASYSN
                     VRRFLNMGSTIPPDAVRIEELLNYFNFGYDDPPAGSCFTLRSRLSECPWNPGNRLLFL
                     QVCARKIDPSRIPPANLVLLIDISGSMDLPNKLPLLKSAFGLLINNLRAIDTVSIVTY
                     GSSVSVALQPTSGENKKKILEAINDLEPGGATPGEAGIRAAYRLAKSQLIKGGNNRVI
                     LATDGDFNEGETSEEELARLVDQYKNWGVYLTCLGVGMGNYKDSKLEALAKKGNGNFA
                     YLDEEQEAEKVLVREFTQTVYAVADDVYLNIQFNPGLVKDYRLIGFDNKVKALSDTLS
                     GLQGGEVGSGHSLLALFEIVPGDPDPSVVGRERLAEVLLNYRLPNERVARTSSFDCSS
                     RVIPFRELQPCYRFASSIALFGGLLKKSPFTRQADWKDAIEMAQSSVDPGDPMQEEFI
                     TLIEKARKIYGRNKHRRKDQY"
     gene            complement(3063..4217)
                     /gene="dnaJ"
                     /locus_tag="VGQ51_10240"
     CDS             complement(3063..4217)
                     /gene="dnaJ"
                     /locus_tag="VGQ51_10240"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV9036993.1"
                     /translation="MSKRDYYETLGVAKGANADEIKKAYRKVAMQFHPDRNPGDKPAE
                     EKFKEAAEAYEVLSDADKRAQYDRYGHAGLSGNGRGFSGGGMNMDDIFSQFGDIFGDD
                     IFGSFFGGRRGGGGARTRGVRGSNLRVKIKLNYEEIARGVSKNIKVKKYVACGTCAGS
                     GAKDRNSVQTCSTCGGSGQVRRVTNTFLGQMQTVTTCPTCNGEGSIVTAKCTVCKGDG
                     RVYGEETVTIDIPAGVQEGMQLSVGGRGNAGERGGPPGDLIILIEEEQHPDLHRDGLN
                     VAYELYISFTDAVFGLQAEVPTIDGKAKIKIPAGTQSGKIFRLKGKGFPGVNSYERGD
                     QLIHINVWTPQHVTPEEKAMLDKLNDSPNFQPKPDKNERSFFDKVREMFS"
     gene            complement(4241..4801)
                     /locus_tag="VGQ51_10245"
     CDS             complement(4241..4801)
                     /locus_tag="VGQ51_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV9036994.1"
                     /translation="MKDYNNEDQGQEDLEDLNTDENIAGATRLDDDISAREEEFERLK
                     GDLQEQKDKYLRLFAEFDNFRKRNARENLELRQTAGKEVIVSLLDVLDDCDRAEKQLD
                     LAGDDLTAKEGVRLVFSKLRSALYAKGLKPMQSIGMEFDVEKHEAITEVPVQDPALKG
                     KVIDEVKKGYYLNDKIIRFAKVVVGK"
     gene            5028..5594
                     /locus_tag="VGQ51_10250"
     CDS             5028..5594
                     /locus_tag="VGQ51_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9036995.1"
                     /translation="MWSRSLGGDTMAFRDIHRVLFKGLYNYALKLLQDGGLAGDAVQD
                     LFVRIWVRRDQIGVIQKVKPYFFTALRRQVLNQLRNLHLREKQISGLPRPDIEFSPEE
                     IVVRNEEYENLQHRIAALLNKLPRRQKEVIYLRYFEEMENTQIAEVMGIHYQSVLNLT
                     QKALQKLRSADLLAVFYSLVTLYRWCRG"
     gene            5704..6624
                     /locus_tag="VGQ51_10255"
     CDS             5704..6624
                     /locus_tag="VGQ51_10255"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEV9036996.1"
                     /translation="MDDVTNNEMENNRLPFGFRSRPVSDELREANWRAVEEGMRRNET
                     APVRAIRGRVIRWVRVSAVAASLAVVATGVWWIANRTGHAQYAVIRTGYGEIKNIILP
                     DSSVVTLNANSSMRMPEQWTDADGRQVWLEGEAYFQVRKKPSTVEKFVVHTRQVDVEV
                     LGTRFNVNTRREQAVVSLEEGKVRLSMHGSDTVVLEKKAGMILRPGQVVVVGADHRTS
                     INEDKDVATHSGWTRHAFHFDGTTLGEIATMVEDTYGYKMEVEDPAMLRFTISGDVRV
                     RNIDEMVKVLEASTGYAMRTGNNIIHVSYH"
     gene            6653..>7023
                     /locus_tag="VGQ51_10260"
     CDS             6653..>7023
                     /locus_tag="VGQ51_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HEV9036997.1"
                     /translation="MRNETLRGKLLLFALFFLGSSGLMNARSQTNAWLTSYSQVNVSG
                     AGLHDQRSQARTISSILQDARKTFDVDFIYETKVLPGARVIIDVDKYKSVEDFLDDLL
                     RPYNLKYKKVLSKAYVIYSSSP"
BASE COUNT         1544 a         1580 c         2181 g         1718 t
ORIGIN      
        1 tccctcggat tatcagggtt gggcgtatgg attgaagaag gcaggttatg cgacgaatgt
       61 acggtatccg cagatattga tcaaactgat aaagggctat aacctgcagc aatatacgct
      121 gatagcgatg ggtaagatga agccggagga ggaggtggtt ttgaatatgc cggggctgcc
      181 gccggcgggg caggtggtgg tggaggggaa ggtggattcg ggtgtggcgg acaggccggc
      241 ggtgacggac ccggcagcgg cggcggcgta tcctgtcggg gagttcacga tcaacaagac
      301 gagggtcgtt tatgtgaagg caggggtttc gctgctggcg gtggccgggc agtacgatgt
      361 cccgctgccg cgtttgttgg aattcaatga tatgaaggag gaggatgtat tagtgaaagg
      421 acaactcgtc tatctgcaac ggaagaggag gaccggggcg aatgaatatc atgtgatgcg
      481 ggacggggag agtctttacg atgtcagtca agccgaggga atccgattgg aggatatgct
      541 ggagatgaat cagctgacgc ccggcgagcg gccggctccg ggggagcgaa tctatttgca
      601 gggttcggcg ccatcacgac caagattatt atgatatgtc tgttttaaaa gtacacgata
      661 aagaattcaa gccgtatttg ggggctgagc tgatcgatga gaagatagcg gcgatagcga
      721 ccaggatcga cgaggactat gctggaaagc ggccattgtt catcgccatt ttgaatggcg
      781 cgttcatgtt cgcttccgac ctgttcaaga agatcagtat cgaatcggag atcagtttta
      841 tcaaactggc gtcgtataag ggtaccaaat cgtcgggcca ggtgatcacc gccattggat
      901 tggataccga tctgcatggg aggcatgtgg tgatcgtgga ggatatcgtc gatacgggga
      961 agaccctgag tgaattcctt ccgcaactgg agcatcagca gccggcgtcg ctgcggatcg
     1021 cggccttatt gcagaagccg gaggcaacgg tgtatccgat ccgggtggat tacctggggt
     1081 tttcggtgcc gaataagttc ctgcttggct atggtcttga ctatgatgga cttgggagga
     1141 atcttcccgc tatttatcag ttagtcgaag gaagtgagta aggaacttga caaagagcgg
     1201 agaacgtatt aattttaaat aaaatatccg attgaaaccc gcattacttc tgctagtggg
     1261 ttctttgttc ttttatactt cgcgcgcgca gttctatttg cgcggagagg tgaaagatga
     1321 gaacaacagc ctcttgccgg atgtgaggat actgcttcat tccagcggat atctatatca
     1381 ttcgggtagt tcgggggcct tcgggatacc tataccttcc tcagctgata cggttacact
     1441 ttcggccgat ggttttcaga cgctggtgat gccggtggat gccaaccggt atcaattcat
     1501 caaaatgcga cctgtgggcg gcgcggcgcc gtcggcgcct atgcgacggg gtttgttatc
     1561 gctgaccaaa gacgtcaagg cagcggaccg aaaggattgg actgtgggga gcgagacata
     1621 cagctctctg gccgagaaca attttattcc tgcgctgcgg tttcccgaga cgggatttgc
     1681 agtcaccatc gacaaggcct cttacagcaa cgtgcgccgt ttcctgaaca tgggcagtac
     1741 gatccctccg gatgcggtga ggatcgaaga gttgctgaat tatttcaact tcgggtatga
     1801 cgatcccccg gcgggcagtt gttttacctt gcgttcgcga ttgtccgaat gcccgtggaa
     1861 cccgggcaac cgtttgcttt ttttgcaggt gtgtgcgcgg aagatagatc cgtcgcggat
     1921 ccctccggcc aacctggtgc tgctgatcga tatatcggga tcgatggact tgccgaacaa
     1981 gctgccgttg ctgaaatcgg cttttggttt attgatcaat aacctgaggg caatcgatac
     2041 ggtatcgatc gtgacctacg ggagctcggt ctcggtggcc ttgcagccga catcggggga
     2101 gaacaagaaa aagatccttg aggcgataaa cgatctggag ccagggggcg caactcccgg
     2161 cgaggcgggg atccgggctg cttaccggct tgccaaaagc caattgatca aaggcggtaa
     2221 caatcgggtg atcctggcta cggatggcga cttcaatgaa ggggagacat cggaggagga
     2281 attggcgagg ctggtagatc aatacaagaa ttggggcgtc tatctgacct gtctaggggt
     2341 agggatggga aattataaag actcgaagct ggaggcgctg gctaagaagg gaaatgggaa
     2401 tttcgcgtat ctggatgagg agcaggaagc ggagaaggtg ctcgtgcggg aattcacgca
     2461 gacggtatat gcggtagctg acgacgtgta tttgaacata caattcaatc ccgggctggt
     2521 gaaggattac cggctgatcg ggtttgacaa taaggtaaaa gcgttgtcgg atacgctgag
     2581 cgggttgcag gggggagaag tgggttcggg gcattcgttg ctggctttgt tcgagatcgt
     2641 gccgggtgat cccgatcctt ccgttgtcgg tcgggaacgg ctggcggagg tgttgttgaa
     2701 ttaccggttg cctaatgaga gggtggcgcg gacgagctcg ttcgattgca gcagccgggt
     2761 gatccctttc agggagctac agccttgtta tcgttttgcc tcgtcgatcg cgttgttcgg
     2821 ggggttgctt aagaagtcgc cttttaccag gcaggcggat tggaaggatg ccatcgagat
     2881 ggcgcaaagt tctgttgacc cgggcgatcc catgcaggag gaattcatta cgctgatcga
     2941 gaaggcgcgg aagatatatg ggcgtaataa gcatcgtcgc aaggaccagt actgagcgga
     3001 gcgcgcatga ggactagcct ggggcacatg aggactggcc tggggcgcga agccgtggtg
     3061 tgtcaggaga acatttccct tactttatcg aagaagctac gctcattctt atcgggcttg
     3121 ggttggaaat tgggtgagtc gttcaatttg tcgagcatgg ccttttcctc gggggtgacg
     3181 tgctggggtg tccagacatt gatgtgaatg agctggtcgc ctctttcgta ggagttgacg
     3241 ccgggaaagc cctttccttt caggcggaag atcttgccac tttgtgtacc tgcgggtatt
     3301 ttgatcttag ccttgccgtc gatggtgggt acttccgcct gtaagccgaa tacggcatcg
     3361 gtaaaggaaa tatagagttc gtatgcgaca ttgagtccgt ctctatggag atcgggatgt
     3421 tgttcttctt cgatgagaat gatcagatcg ccgggggggc cgccgcgttc gccggcgttg
     3481 cctctgccgc cgacgctgag ctgcatgcct tcctgtacgc ctgcggggat atcgatggtg
     3541 acggtctctt cgccatatac gcgaccgtcg cctttacata ccgtgcattt ggcggtgacg
     3601 atagagcctt cgccgttgca ggtggggcag gtggtcaccg tctgcatttg tcccaggaag
     3661 gtgttggtga cgcgtctgac ctggccgctg ccgccgcagg tggagcaggt ctgaacgctg
     3721 ttccggtctt tggctccgct gcctgcgcag gtgccgcagg cgacgtattt tttgaccttt
     3781 atattctttg agacgcccct ggcgatctct tcgtaattga gcttgatctt tacgcggaga
     3841 ttgctgcctc tgacgccgcg ggtccgtgcg ccaccaccac ctctgcggcc gccgaaaaag
     3901 ctgccgaaga tgtcatcgcc gaaaatgtct ccgaactgac tgaagatatc gtccatgttc
     3961 atgccgccgc cactgaagcc gcggccgttg ccggagagac cggcgtgtcc gtagcggtcg
     4021 tactgggcgc gtttgtcggc gtcgctgagc acctcatagg cttctgcggc ctctttgaat
     4081 ttttcttcgg ccggtttgtc gccgggattg cggtcgggat ggaactgcat ggctacctta
     4141 cggtaggcct tcttgatctc gtcggcattg gcgcctttcg ctacgcccag tgtttcgtaa
     4201 taatctcttt ttgacataga ctcttagcag gatgttgggt ttatttgcct accactactt
     4261 tggcaaaacg aatgatctta tcattgaggt agtagccttt tttcacttcg tcgatcactt
     4321 tacctttgag ggcgggatcc tgaacgggta cttcggtgat ggcttcgtgc ttttcgacgt
     4381 cgaattccat cccgatgctt tgcatgggtt tcagcccttt ggcatacagg gcggagcgga
     4441 gcttggaaaa gaccaggcgg accccttctt ttgcggtcag gtcatcaccg gccagatcga
     4501 gctgtttttc ggcgcggtcg cagtcgtcga ggacatcgag cagggagacg atgacttctt
     4561 ttccggccgt ctggcgaagc tcgaggtttt ctcttgcgtt gcgtttcctg aaattatcga
     4621 attcggcaaa gaggcgaagg tatttatctt tttgttcctg aagatctcct ttcaggcgtt
     4681 cgaattcctc ttcgcgggcg gaaatgtcgt catccagccg ggtagctcct gcaatatttt
     4741 catcagtgtt cagatcctcc aggtcttcct gtccctggtc ctcgttattg taatctttca
     4801 tagtcgtaaa aattggcaca aaggtaagat tgtcaatatt cctgccaagc atgatttaga
     4861 gacaaaatgt cttaaaaaac cctatcgact gacagtggcc gtgcgggtat cgaccctaaa
     4921 agttattttt gtcgtcagga cttgagccgg gcaccggtcc ggccaagtgg gtccttcgaa
     4981 ttaactaaat ggatactaat ccgttatatt cagaaaattt agaggaattg tggagccggt
     5041 ccctgggtgg ggatacgatg gcctttcggg atatccaccg ggtattgttc aaaggactgt
     5101 acaattatgc tctgaagcta ttgcaggatg ggggtctggc cggggatgcg gtacaggacc
     5161 tgttcgtgcg gatatgggtg cgccgggatc agatcggcgt catccaaaag gtgaagccat
     5221 atttttttac cgctctccgc cggcaggtgt tgaatcagct gcggaatctt catttgcggg
     5281 aaaagcagat cagcggtctt cccaggccgg atatcgaatt ttctccggaa gagatcgtag
     5341 tgcgtaatga ggaatacgag aatcttcaac ataggatcgc cgcgttgctg aataaattgc
     5401 cgaggcgtca gaaggaagtc atttatctgc gttatttcga ggaaatggag aatacccaga
     5461 tcgccgaagt gatgggtatc cattatcaat cggttttaaa tctcactcaa aaggccctcc
     5521 agaaattacg atcggccgat ctgctggccg tgttttattc gctggtcacg ttgtaccggt
     5581 ggtgccgggg ataaggtgcg ggcacgttgg atgccggtgc gggcacgtga acggtcgaaa
     5641 tattttttaa gaaagagggt ataaaaactc cgaccgggtg tcttgaagga taaaaatgcc
     5701 ttcatggacg atgttacaaa caatgaaatg gaaaacaatc ggctgccatt cgggtttcgt
     5761 tcccgaccgg tgagcgacga gcttcgggag gccaactgga gggcggtgga agaggggatg
     5821 aggagaaacg agactgcacc ggtgagggct atccggggaa gggttatccg ctgggtgagg
     5881 gtatcggcag tggctgcatc cctggctgtc gttgcgacag gtgtatggtg gatcgccaac
     5941 cgtaccgggc atgcccagta tgccgtcata cggacgggtt atggagagat caagaacatt
     6001 atccttcccg acagttcggt cgtcaccctg aatgccaatt cttcgatgcg tatgcccgag
     6061 cagtggacgg atgcggatgg gcgccaggtt tggctggaag gagaggcgta tttccaggtg
     6121 cggaagaagc cgtcgaccgt ggagaagttt gtcgtacata cccgtcaggt ggacgtagag
     6181 gtgttgggga cccggtttaa cgtcaacacg agaagagagc aggcggtagt ttcgctggaa
     6241 gaggggaagg tacgtctgtc gatgcatggg tccgatacgg tagtgctgga gaaaaaagcg
     6301 gggatgatat tgcggccggg acaggtggtc gtcgtcggcg cagatcacag aacaagcatc
     6361 aatgaagata aggatgtggc gacgcattcg ggctggacga gacatgcctt ccatttcgac
     6421 ggtaccacgc ttggcgagat cgcgacgatg gtagaggaca cctatggata taaaatggag
     6481 gtggaagatc cggcgatgct tcgattcacc atatcagggg atgtgcgtgt gcggaatatc
     6541 gatgaaatgg tcaaagtgct ggaggcgtca acgggatatg cgatgcgtac cgggaacaat
     6601 atcattcatg tgagctatca ttaaaccaaa caccagttaa aacaaacgcc atatgagaaa
     6661 tgaaaccttg aggggtaagc tgctgctttt tgccctgttc tttctgggct cgagcggcct
     6721 gatgaacgcg cgttcacaga caaatgcctg gctgacgtcc tattcgcagg tcaatgtgtc
     6781 tggggccggc ttgcacgacc agcggagcca ggcccgcact atctccagca tcctgcagga
     6841 cgccaggaag accttcgatg tggacttcat ttacgagacc aaagtgttgc ccggtgcgcg
     6901 ggtgatcatc gacgttgaca aatacaaatc cgtcgaggat tttttggacg atctgttaag
     6961 gccctacaac ctgaagtaca aaaaagtgct gagcaaggct tatgtcattt attcgagcag
     7021 ccc
//
LOCUS       DASYSV010000343         5477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2498263, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000343 DASYSV010000000
VERSION     DASYSV010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2498263"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VGQ51_10265"
     CDS             complement(<1..1196)
                     /locus_tag="VGQ51_10265"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9036998.1"
                     /translation="MILQRMARYSLALLLVIFYHPTLFAQVNQRVITGKVINSDTKEP
                     MNNVSVTEKGSARGVTTDESGSFSLKVPGNGTVILSFSYIGFDPKDVKTKGDSAMLVS
                     LVNSNKKLDDVVVVGYGTQKRSNILGAVATVDPKEVQDLPVANLSTALVNLVPGVSVN
                     QSAGKPGASTTLSIRSPVSLNSQGTGPLYVIDGLAPIIGSGAGIDPTGKTAFDALDAS
                     EVESITFLKDGMATIYGARGANGVVLVTTKHGRPGKPRINYSGSYSNTRANKIPTMID
                     GYNQALLLNNWVENYAPWKVPTTEIYTPDELDTIKSRQFDWFKNEWIPNGSIQRHNVS
                     VSGGSEKLTYFAGANYYNEIGNLPGVIANRYGMQLGMTAKIIPGLTSEVSVNYNTGYL
                     NRPAVK"
     gene            complement(1231..1779)
                     /locus_tag="VGQ51_10270"
     CDS             complement(1231..1779)
                     /locus_tag="VGQ51_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3826 domain-containing protein"
                     /protein_id="HEV9036999.1"
                     /translation="MNSDEDTAYVRVITARAGKIVAALGLTDSTLFYKVRDIVAGQYY
                     ALNAVYTDRDERLKQLKNQSDKNLTDSLKKTIQQDVDEKIGHLHTAYLGRLATVVSPE
                     QIDQIKDGMTYSVLEVTFRGYLKLWPQLTDEQKGVILEDLTEAREHAMDAESSEKKHW
                     WFGKYKGRINNYLSSQGLQQKK"
     gene            2025..2594
                     /locus_tag="VGQ51_10275"
     CDS             2025..2594
                     /locus_tag="VGQ51_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEV9037000.1"
                     /translation="MPRLTFSLLALAAISLSQPSHAQSSDDMKAMMAYSTPGDSHKML
                     AKMAGTWSATVTFWMPGNATPMTSTATAVNEMILGGRYLQSKNSGNMMGQPFEGIGVT
                     GYDNAKKVYLATWIDNFGTGIMTMTGSWDEASKAIVFTGTEVDPASGKDYPIRQVVRN
                     PDDNTQVMEMYATMGGKEIKTMEIKYVRK"
     gene            complement(2600..>5477)
                     /locus_tag="VGQ51_10280"
     CDS             complement(2600..>5477)
                     /locus_tag="VGQ51_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEV9037001.1"
                     /translation="GMLSEGMAFTDKDPTTPHEWQRLDHPILTPRDPDVAWWDYHTMY
                     KSWVMHDPQRLTGHPFILFYNANGDSLDKRRGSERIGMAVSDDMIHWTRPRRDPLLDH
                     LTGITGDPYIQKMGNLYVMFYFGAFWKGTHGAFNRFACSYDLVHWTDWTGPDLIESSE
                     PYDDVFAHKSCVVKYKGVVYHYYCAVDKKGDRCIALATSKDLVSHERVSFDAGWEFHL
                     GADTAGGKWRPVRLPHDWSIEGSFSAGNATGQGEGGLPAGVGWYRKEFRVAGDSAHIF
                     IDFDGIYRNSEVWINGHLLGSRPNGYISFRYELTPWVRRDKSNVLLVRVDNSAQPNSR
                     WYSGSGIYRHVWLEEKGSVSIDHWGEHVTTPEVSGKMARVHVETSGGGAPEARNVLYD
                     SAGQVVAMTAGEDLSVPNPRLWSPEHPYLYRLETSVYDGDRLLDRVVTPVGIRRFRFD
                     VQQGFFLNGRPLKILGVCMHHDLGALGAAVNTAAIRRQLRILKEMGCNAIRTSHNPPA
                     PEFLDCCDEMGFIVMDEAFDMWQKKKTKYDYSLDFGEWHRRDLEDQVLRDRNHPSVMI
                     WSIGNEIREQFDTSGIRIARELAGIIRSLDTTRPVTSALTETDTAKNFIYRSGALDLI
                     GLNYHQEQYDSALQHYPGHPVIATETTSALETRGHYDDDMPSDSIRRWPAGPKKPLTT
                     GNPDYTVSAYDNVSAYWGSTHEETWKVVKRDPFISGLFIWTGFDYIGEPTPYPWPARS
                     AYFGIVDLAGFPKDVYYLYQSEWTSKPVLHVFPHWNWRPRQPVDVWAYYSQADEAELF
                     LNGRSLGIRRKQGDALHVQWRVPWQPGTLRVVTRRNGHVVLEQQVHTAGMATQIRLET
                     DRSVVDADGSDLAFVTIRITDGKGVTVPDAGNDLHVTVEGPGELAAMDNGYQADLESF
                     HGSHHKAYNGLCLAIIRTKKQAGRIVVRVSGKGLQPATLVLNSR"
BASE COUNT         1208 a         1556 c         1426 g         1287 t
ORIGIN      
        1 ctcttcacag cgggccggtt caaatagccg gtgttgtagt tgacgctgac ctcggaggta
       61 agaccgggaa tgatcttagc cgtcatgccg agctgcatcc catagcggtt agcaattacg
      121 ccgggcaggt tacctatctc attataataa tttgcgcccg cgaaataggt cagtttttcc
      181 gagcctccgc tgacgctgac attatgccgt tggatcgagc cattgggtat ccactcattt
      241 ttgaaccagt cgaattgacg cgacttgatc gtatccagct catcgggagt atagatctcg
      301 gtcgtgggaa ccttccatgg cgcataattc tctacccagt tgttcagcaa cagcgcctga
      361 ttgtaaccat cgatcatggt cgggatcttg ttggcccggg tattcgaata ggagccggaa
      421 tagttgatcc tcggttttcc ggggcggccg tgtttggtcg tgacgaggac gacgccattg
      481 gcgccgcgcg ctccatagat ggtagccatt ccgtctttca ggaaagtgat gctttccacc
      541 tcggaagcat cgagcgcatc gaaggccgtt tttccagtcg ggtcgatccc cgcgcccgag
      601 ccgatgatcg gcgcgagacc gtcgataaca tataagggac ccgtcccctg cgaattgagt
      661 gaaacggggc ttcggatgct cagggtagtg ctggcccccg gtttccccgc agactggttg
      721 acgcttacac ctggaaccag gttcaccagc gcagttgaca aattggccac cggaagatcc
      781 tgaacctcct tgggatccac cgttgcgacg gcgcccagga tattcgaacg cttttgcgta
      841 ccgtaaccga cgactaccac atcgtccagc ttcttattgg aattgaccag cgacaccagc
      901 atcgccgaat cgcccttcgt cttaacgtcc ttcgggtcga acccgatgta agaaaaagac
      961 aggatgacgg tcccgttgcc aggcactttc agcgagaatg aacccgattc atcggtggtc
     1021 actcccctgg cggaaccttt ttctgtaacg gaaacattgt tcatcggctc tttggtgtcc
     1081 gagttgatca cttttccggt gatcacacgc tgatttacct gtgcgaacag ggttggatga
     1141 tagaatatca ctagcaggag tgcgagggag tatctcgcca ttctttgcag gatcatatgg
     1201 catttagttt taggagaatt atgcaatcat ttattttttc tgttgtaacc cctgtgaaga
     1261 caggtaatta ttgatacgtc ctttgtactt tccgaaccac cagtgcttct tctcgctgga
     1321 ctccgcatcc atcgcatgtt cgcgcgcctc cgttaaatcc tccaggatca cccctttttg
     1381 ctcatctgtc aattgtggcc acagcttcaa atacccgcga aaggtcactt caaggacgct
     1441 gtaggtcatg ccgtccttga tctgatcgat ctgctccggg gagaccaccg tagccagtct
     1501 gccgagataa gccgtatgca gatggccgat cttctcatcg acatcctgct ggatcgtttt
     1561 tttgagtgaa tcggtaagat tcttatcgga ttggtttttc agttgtttca gtcgttcgtc
     1621 ccgatcggta tacactgcgt tgagtgcgta gtattgtccc gcaacaatat cgcgcacctt
     1681 ataaaataac gtcgagtcgg tcaggcccaa tgccgcaacg atcttgccgg cccttgccgt
     1741 gatcacgcgg acataagcgg tatcttcatc ggagttcact ggatttacgc acgggttggc
     1801 ctgagagacc aatacattta ggatcattgc gagtgccgat ccccaaacaa gcaaacgtac
     1861 cttcataatg tcttaaccat ttatcggaaa gactaaaggt acaggcgtga tgaggggtaa
     1921 aacaatgtaa ggtttgacta tgttgatgtc atatcttact attttcgggc aggttggagg
     1981 tatcaaaacc tttgcgtaac ttttacgtaa ctaaaactac ttttatgccc aggctaacct
     2041 tctcgctgct ggcgcttgcc gccatttccc tatcccaacc ctcccacgct caatcttccg
     2101 acgatatgaa agccatgatg gcttattcca ctcccggcga cagccataag atgctggcca
     2161 aaatggcggg cacctggtcg gccactgtca cattctggat gccgggcaac gcgaccccga
     2221 tgacctccac cgctaccgcc gtcaatgaaa tgatcctcgg cggtcgctac ctgcaatcca
     2281 aaaattcggg gaacatgatg ggccaaccct tcgaaggcat cggcgtcacc ggctatgaca
     2341 atgccaaaaa agtttatctc gccacctgga tcgacaattt cggaacgggc atcatgacta
     2401 tgacaggttc ctgggatgaa gccagcaagg ccatcgtctt tacgggcacc gaagtcgacc
     2461 ccgcatcggg aaaagactat cccattcgcc aggtggtccg gaacccggat gacaacaccc
     2521 aggtcatgga gatgtatgcg accatgggag gaaaggaaat caagaccatg gagatcaagt
     2581 atgtgaggaa ataacctcct cacctgctgt tcaacaccag cgtagccggc tgcagccctt
     2641 tcccgctaac ccggacgacg atcctgccgg cttgcttttt cgttctgatg atcgcaagac
     2701 ataatccatt atatgccttg tgatgcgacc catggaaaga ttccaggtcc gcctggtacc
     2761 cgttatccat tgcggcaagc tcgccggggc cttctacggt cacgtgaaga tcgttgccgg
     2821 catcgggtac cgtaacacct tttccatcgg taatgcgtat cgtcacaaat gccagatccg
     2881 acccgtctgc atcgaccacc gacctgtcgg tttcgagccg gatctgtgtg gccatacctg
     2941 ccgtatgcac ttgctgctcc aatacgacat gcccatttcg ccgcgtcacc acgcgcaggg
     3001 ttcccggttg ccagggcacc cgccattgaa catgcagcgc atcgccctgt ttcctgcgaa
     3061 tgccgagact gcgcccgttg aggaacaact cggcttcatc ggcctgacta taataggccc
     3121 acacgtcaac gggttgccgc ggacgccagt tccagtgcgg gaaaacatgc agcaccggtt
     3181 tggaagtcca ttccgactga tagaggtaat atacatcctt ggggaaaccg gccagatcga
     3241 ctatgccgaa ataggcgctg cgggccggcc atggataagg cgtcggttcg cctatgtagt
     3301 cgaatccggt ccagataaaa agtccggaga taaagggatc ccgtttgacc actttccagg
     3361 tctcctcatg cgtggaaccc caataggccg atacattgtc ataggcagag acggtataat
     3421 cgggattgcc ggtggttaat ggttttttgg gccctgccgg ccagcggcgg atgctgtcgg
     3481 acggcatgtc gtcgtcataa tgtccccggg tttcgagcgc agaggtcgtt tcggtggcga
     3541 tgacgggatg cccgggatag tgttggaggg cgctgtcgta ttgctcctgg tgatagttga
     3601 gaccgatgag atccagcgcg ccgctgcgat aaatgaaatt cttcgccgtg tcggtttcgg
     3661 tgagcgccga tgtcacgggt cgcgttgtgt cgagactgcg aatgatccct gccagctcgc
     3721 gggcaatgcg aatgccgctg gtgtcgaatt gctccctgat ctcattgccg atgctccaga
     3781 tcattaccga tgggtgattg cggtcgcgaa ggacctggtc ttcgagatcg cggcgatgcc
     3841 attccccgaa gtcgagcgaa tagtcgtatt tcgttttttt cttctgccac atgtcgaagg
     3901 cctcgtccat cacgatgaaa cccatctcat cgcagcaatc gaggaattca ggagcggggg
     3961 gattatgcga agtccggatg gcattgcagc ccatctcttt caggatacgc agttgccggc
     4021 gaatagcggc agtattgact gctgcgccga gggcgccgag atcatgatgc atgcagacac
     4081 cgaggatctt cagcggacgg ccattcagaa aaaaaccttg ttgaacgtca aaccggaagc
     4141 gccggatgcc caccggagta accacgcgat ccagcaacct gtccccatca taaacactgg
     4201 tctccagcct atacagatac gggtgttcag gagaccaaag ccgcggattc ggaacgctga
     4261 gatcctcccc cgccgtcatt gctactacct gccctgccga atcatagagc acattccgcg
     4321 cctccggcgc tccgcctcca ctcgtctcta catggacccg cgccattttc ccgctgactt
     4381 cgggcgtcgt gacatgctct ccccagtggt cgatggatac tgaacctttc tcctccagcc
     4441 acacatgccg gtagatgccc gaaccggaat accatcgcga gttcggttgc gcggagttgt
     4501 ccaccctgac cagcagcaca ttgctcttgt cacgcctgac ccagggcgtt agttcgtacc
     4561 ggaaggaaat atacccgttg ggccgacttc ccagcaagtg accattgatc caaacttcac
     4621 tgtttcgata gatgccatcg aaatcgataa aaatatgcgc ggaatctccg gccacgcgaa
     4681 actccttacg ataccaaccc actcccgccg gtaaccctcc ttcgccctgc cctgtagcgt
     4741 tccccgcact aaaactgcct tcgatgctcc aatcatgagg cagccggacc gggcgccact
     4801 tacccccggc agtgtcggcg cccaaatgga attcccagcc tgcatcgaaa ctcaccctct
     4861 catgcgatac gagatccttc gatgtcgcca gcgcgataca acgatccccc tttttgtcca
     4921 cggcacagta ataatgatag acgacaccct tgtatttcac tacacaggat ttgtgcgcga
     4981 atacatcgtc atacggctcg gaggattcga tcaggtcggg tcccgtccag tcagtccagt
     5041 gcaccagatc gtacgagcag gcaaaccggt tgaatgcgcc gtgtgtcccc ttccagaaag
     5101 cgccgaaata gaacatgaca tagagattgc ccatcttttg aatatagggg tcgccggtga
     5161 tgccggtcag atgatcgagc agcgggtcac ggcgcggcct ggtccaatgg atcatgtcgt
     5221 ccgagacggc cattccgata cgctcgcttc cgcgtcgttt gtcgagactg tcgccattgg
     5281 cgttgtagaa caggatgaat ggatgaccgg tgagacgttg cggatcatgc atgacccagc
     5341 tcttatacat cgtatgataa tcccaccagg cgacatccgg gtcgcgcggt gtaaggatgg
     5401 ggtgatccag ccgctgccat tcgtgaggcg tcgttggatc tttatcggtg aaggccatgc
     5461 cctcggaaag catgccc
//
LOCUS       DASYSV010000344         7977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_838493, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000344 DASYSV010000000
VERSION     DASYSV010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838493"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VGQ51_10285"
     CDS             <1..197
                     /locus_tag="VGQ51_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEV9037002.1"
                     /translation="QTQKIDFFVFGHRHLPIDFLLNAEGSRYINLGDWIRYYTYAVLD
                     GGTMTLLSETPGTADKIVRL"
     gene            194..1033
                     /locus_tag="VGQ51_10290"
     CDS             194..1033
                     /locus_tag="VGQ51_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037003.1"
                     /translation="MTRRYLYMTLLLLLVRGSASAQEANPPVTAAPGDFQLTLTIPQD
                     IVDFTVDNLGNIYILNSDNQLKKLGPKGDSLAVFNDVRQYGKIASVDATNPLKILVYY
                     REFTTVIELDRFLNIVNTIDLRKQNILQARAIGLAYDNNIWVFDELDARLKRIGDDGS
                     LVDQTTDFRQLFDSVPDPTIIRDQGGYVYLYDPSKGVYIFDHYGAMKTHLILQGWEDF
                     DVIGKNLLGRDSHNLYRYQPGTLDMQEQPIPAAYSGAISIRITPSVIYVLKKTGLDVY
                     TKK"
     gene            1058..2305
                     /locus_tag="VGQ51_10295"
     CDS             1058..2305
                     /locus_tag="VGQ51_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEV9037004.1"
                     /translation="MHDFWNIVILDNPLRSYIIVAATILFVIALKRYLSRLIARLIFR
                     VIRRMGSGLDRSAFLDFVLRPIGTFLVVFVSITSIEKLHFPRQLDFDIYEVPSKTMMH
                     GLASIILIVTFIWLLLRLVDFIATVLRNKARKGGGPRDNQVIVFIRDFLKAILGIIGL
                     LMVLDAVFKVDVSSILTSLGLAGAAIALAAKESIENLIASFVIFFDKPFKSGDYLKVN
                     AISGTVEKIGLRSTRIRTDQKTYVTVPNKQMVDSVVDNLTLRTQRKGELRLELSLSTS
                     AALLDDLISGIREILSIPVIEDKVVFLNDITGSAFLVNVDYFTAPLAIETFNATKQQV
                     NLEVLRLMEKLKISIAGSSTTISLTSVNPGPDAPAAPGASPASRSRPAGPHSPASPGA
                     SPASRSRPAGPHPPASSGPDAKT"
     gene            complement(2295..2807)
                     /locus_tag="VGQ51_10300"
     CDS             complement(2295..2807)
                     /locus_tag="VGQ51_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEV9037005.1"
                     /translation="MKKLSLVAIFIGLGMLTRAQTEYEVSSDGTYKILKGSISREMLE
                     NDTAFRWFHDNQTGYVPNPETVSILKAKGSNLRFVVFFGTWCDDSRNLLPKFYALMDA
                     ASIGNDQIMLIGVDHQKKSTTHLPEVMHLTNTPTFIILRGDEEEGRVVEYGKNGQWEK
                     EIGDIVAAKF"
     gene            complement(2819..3571)
                     /locus_tag="VGQ51_10305"
     CDS             complement(2819..3571)
                     /locus_tag="VGQ51_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV9037006.1"
                     /translation="MSNLNQISLDFGPEHQGPPSPPAGVIGTSGDGADGYMAAEGVGV
                     RLKSYKRTTVSPLPEKPEPVNGNLFGNGEQVAEVVAPAVAPEPKVAPEPKVAPEPKVA
                     PEPKRIARGARKPVPKKVPSKRGRKSLKQVSAEADLIEIPEDEVLFSKQYYTMGEVSE
                     MFRVNQSLLRFWETEFDILQPRKNKKGDRYFRPIDIKNLHLIYHLLRQRKYTIEGAKD
                     FLKKNKKAEERFEVIRRLQEIKAFLLEWKAQL"
     gene            complement(3627..4598)
                     /locus_tag="VGQ51_10310"
     CDS             complement(3627..4598)
                     /locus_tag="VGQ51_10310"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV9037007.1"
                     /translation="MKKIKYYYNTNTLRYEKLETPLRVTLLRVLGFLSAAVVTALILV
                     SIAYKYFPSANEKRLMQRNDALQDNYYTLNDKVTKLQEQMGELEKRDNNVYRAIFEAA
                     PIPDSARAKQSRQEQEIKLVQGMDQENLYTSIADTLERLTARMATQAKSYVEIDGFIK
                     NKEQLLACTPAIQPVSNKDLSRIASGFGYRIDPVYKTTKFHAGLDFAAPIGTPIYATA
                     NGTVEVAGNTGNGYGNHVVINHGYGYGTLYGHMFRVKVRVGEKVKRGEIIGWVGSTGK
                     STGPHCHYEVHRNGDPVDPVYYFYNDITPEQYDRLLKLAASSNQSFD"
     gene            4786..7494
                     /gene="alaS"
                     /locus_tag="VGQ51_10315"
     CDS             4786..7494
                     /gene="alaS"
                     /locus_tag="VGQ51_10315"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV9037008.1"
                     /translation="MMTSSEIRKKFLEFFVSKGHTIVPSASIVVKNDPTLKFTNAGMN
                     QFKDYFLGNRKAPYSRVADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDPA
                     DPSAGYFKPEAIAWSWELLTKVYGIPVDRLYVTVFEGDPENNLPADTEAQHEWKKWIA
                     PERILLGSKKDNFWEMGDTGPCGPCTEIHVDCRTDAERAVKDGASLVNNDHPQVIEIW
                     NNVFIQFNRLKDRSLQPLPAKHVDTGMGFERLVRVLQGKHSNYDTDVFTGTIAMVGNI
                     VKKEYTAGDSKRDVAFRVIADHIRAISFTIADGQLPSNTDAGYVIRRILRRAVRYYYS
                     YLDYKQPLLFQLVPLLADQFRDVFPELHQQVDFIAKVMKEEEEAFLRTLEKGLRRIAE
                     VISDARSRPFSVPDAHSKTISTDKAIIPGNVTFELFDTYGFPPDLTELIAGENGMIVD
                     KTGFDAELQQQKTRSRAATAIDTGDWVVLIEDPLAEFVGYDLLESKTRIVKYRKIKAK
                     GKESWQLVLEATPFYAESGGQVGDTGTLTSGMETIPVLDTKKENDLIVHFTDKVPSEP
                     EAPVIARVDRVKRKATEIHHSATHLLHAALRKVLGTHVAQKGSLVNDTQLRFDFSHFA
                     KMTDDELSTVESLVNEKVRENIPVVIRLMPKEVALLSGAMALFGEKYGDTVRVVTIDP
                     GYSIELCGGTHVGATGELGFFRIRHEGAVAAGVRRIEAVGGRAAETFVGEQLAELQAV
                     REVLKNPADVSRAAETLASENADLKKKLEKAEAKQLDILRQRLLQTVQIVGGVAFIGE
                     IVEVPGADSLKKLCFDLKPGLAALPQSTPYLAILAANIDGKAAIALLLDEQLVSARNL
                     QAPAIIKEHVAPLIKGGGGGQKTLATAGGQDASNLAQVIGKVRSLI"
     gene            7592..>7977
                     /locus_tag="VGQ51_10320"
     CDS             7592..>7977
                     /locus_tag="VGQ51_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037009.1"
                     /translation="MNTHLQKMAGVAMLALALNTTGLAQDITPPPTPSGADMDIDTAV
                     NPKHGYDEIIIRRKTDKDAKVTIEIKNGETYINGKPASEYQDGDVSISRRKIRTMRAP
                     YIVMDGNDDMVIKPSPFRNSWSFGGS"
BASE COUNT         1827 a         2300 c         2031 g         1819 t
ORIGIN      
        1 tgcagacgca gaagatcgat ttcttcgtgt tcggtcatcg tcatctgccg atcgattttt
       61 tgctcaacgc cgaaggcagc cggtatatca atctcgggga ttggatccgt tattatacgt
      121 atgcggtcct ggacggggga acgatgacct tgctcagcga gactcccggg acggccgata
      181 agattgttcg tctatgaccc gccgctatct ctatatgacc ctgttgcttc tgctcgtccg
      241 cggatcggca tcggcacagg aagcgaatcc cccggtcacg gccgcgcccg gcgactttca
      301 gctgaccctc acgatcccgc aggacatcgt cgacttcacc gtcgacaatc tcggtaatat
      361 ctatatcctc aacagtgaca accagctgaa gaagctgggt ccgaagggcg attcgctggc
      421 cgtcttcaac gacgtcagac agtatggaaa gatcgcctcc gtcgacgcga ccaatccact
      481 gaagatattg gtctactaca gggaattcac gacggtcatc gagctggacc ggtttctcaa
      541 catcgtcaat actatcgatc tccggaaaca gaatatcctg caggcaaggg ccatcgggtt
      601 ggcatatgat aacaatattt gggtattcga tgaattggat gccaggctca aacgcatcgg
      661 cgacgatggt tcactggtag accagaccac cgatttccgg cagctgttcg actcggttcc
      721 cgatccgacc atcatccgtg accagggggg atacgtctat ctttatgacc cttcgaaggg
      781 cgtttatatc ttcgatcact acggcgcgat gaagactcat ctgatcctgc agggatggga
      841 ggatttcgat gtcattggaa aaaacctgct gggccgtgac agccataatc tttatcgcta
      901 ccagcccgga acgctcgata tgcaggagca gcccattccg gcggcctaca gcggtgccat
      961 cagcatccgg atcacaccct ccgtcatcta cgtgctgaaa aagacggggt tggacgtgta
     1021 taccaaaaaa taggggtcgg gacgtacctt tgcacctatg cacgacttct ggaacatcgt
     1081 catactggac aatccgctga gaagctacat catcgtcgcg gccaccattc tcttcgtcat
     1141 cgccctgaaa cgctatcttt cgcgtctcat cgcccggctg atcttccggg ttatccggcg
     1201 gatgggctcc ggtctcgacc gttcagcctt cctggacttt gtcctcaggc ctatcggcac
     1261 tttcctggtg gtcttcgtct ccattacgtc catcgaaaaa ctgcattttc cccgccagct
     1321 cgatttcgat atctatgagg tcccctcgaa gaccatgatg cacggtctcg cgtctatcat
     1381 tctcatcgtc acgttcatct ggcttcttct acggctggta gacttcatcg ccacggtgct
     1441 gcggaacaag gcccggaaag gcggcggacc gagggataac caggtcatcg tgttcatccg
     1501 cgatttcctc aaggccatac tcggtattat cggtctcctg atggtcctcg acgcggtctt
     1561 taaagtagat gtaagcagta tcctcacctc tctcggtctg gccggcgccg cgatcgcact
     1621 ggcagcaaag gagagcatcg agaacctcat cgcttcgttt gtcatcttct tcgacaaacc
     1681 tttcaagtcg ggcgattacc tcaaggtcaa cgcgatctcc gggacggtgg aaaagatcgg
     1741 cctgcggagc acccgtatcc ggactgacca aaaaacgtat gtcaccgttc ccaataaaca
     1801 gatggtcgat agcgtcgtgg ataacctcac gctgcggacg caaaggaaag gagagcttcg
     1861 attagagcta agcttgtcta cttccgccgc attgttggac gatctgatct ccggtatccg
     1921 tgagatattg tcgataccgg tgatagagga caaggtcgtt tttctcaatg acattacagg
     1981 ttctgcattc ctggtcaatg tggattattt caccgcaccc ctcgcgatcg agacgttcaa
     2041 tgcgaccaaa caacaggtga atctggaagt attgcgattg atggaaaagc tgaagatcag
     2101 tattgcagga tcgagcacca cgatcagcct gacttccgtg aaccccggcc cggatgctcc
     2161 cgcggccccc ggggcttccc ccgcaagccg ttcgcggccc gccggcccgc attcccccgc
     2221 gtcccccggg gcttcccccg caagccgttc gcggcccgcc ggcccgcatc cccccgcgtc
     2281 ctccggcccc gatgctaaaa cttagcggcg acgatgtccc cgatctcctt ttcccattgg
     2341 ccgttcttcc catattccac tacacgcccc tcttcctcgt cccctcggag gataataaaa
     2401 gttggcgtgt tcgtcaggtg catgacttcg ggaagatgcg ttgtcgattt cttttgatga
     2461 tcgacgccga tgagcatgat ctgatcgttg ccgatggacg cggcatccat aagtgcatag
     2521 aattttggca gcaggtttct ggaatcgtcg caccacgttc cgaagaatac cacaaaccgt
     2581 agattcgacc ccttggcttt caatatgctt acggtctcgg gattcggtac atagccggtc
     2641 tggttatcgt ggaaccagcg gaaggcggta tcgttctcca gcatttcacg gctgatcgat
     2701 cccttcagga tcttataggt gccgtcactc gatacttcgt attcggtttg cgcccgggtc
     2761 agcataccca gaccgataaa aatggcgaca agcgagagtt tcttcatgaa gagttttttc
     2821 acagttgcgc cttccattcc aatagaaagg cctttatctc ctggagacgg cggatgacct
     2881 cgaagcgttc ttcggccttc ttgttctttt tcaggaaatc cttcgctcct tcgatggtat
     2941 atttcctttg ccgcaggaga tgatagatga gatggagatt cttaatatcg atgggccgga
     3001 aataccggtc ccctttctta ttcttacgag gctgcaggat atcgaattcc gtttcccaaa
     3061 aacgcagcag gctctggttg acccggaaca tttccgacac ctcgcccatg gtatagtatt
     3121 gcttgctgaa taagacttcg tcttcgggta tttcgataag atcggcttct gcagacacct
     3181 gcttgagcga tttgcgcccc cgtttggagg gcactttttt ggggaccggt ttccgggcac
     3241 ccctggcaat gcgtttcggc tcgggcgcaa ccttcggctc gggcgcaact ttcggttccg
     3301 gcgcaacctt cggttccggc gcaaccgcgg gcgcaaccac ctccgctacc tgctccccat
     3361 ttccaaaaag attaccattc acgggctcgg gcttttcggg caatggagac accgtggtcc
     3421 ttttatagga cttgagccgc acaccgaccc cttccgccgc catatagcca tccgcaccat
     3481 caccggaagt gccaatgaca cctgcaggag gcgaaggagg accctgatgc tccgggccaa
     3541 aatccagcga tatctggttc agattgctca tcgcgtcaaa aagtaggtct tcaaaaatcg
     3601 agccttattc cctgaagtta cgaggattag tcaaaacttt gattgctcga tgccgcgagt
     3661 ttcaacagac gatcatactg ctccggggtg atgtcattgt agaaataata gacgggatcc
     3721 accgggtcgc cattgcgatg tacttcatag tggcagtgcg gccccgtact cttgcctgtg
     3781 cttccgaccc aaccgatgat ctcgcccctt tttactttct cgccgaccct tactttcacc
     3841 cggaacatat gaccgtaaag ggttccatat ccatagccat gattgatgac cacatggttc
     3901 ccatatccgt tgcccgtatt gcccgccacc tctaccgtgc catttgccgt ggcatagatg
     3961 ggcgttccta tcggggccgc gaagtccaat ccggcatgga attttgtcgt tttatagaca
     4021 ggatcgatcc ggtagccgaa accggaggcg atacggctga ggtccttgtt gctcaccggc
     4081 tggatggccg gcgtacaggc caggagctgt tctttattct tgatgaatcc gtcgatctcg
     4141 acatacgact tggcctgggt ggccatccgt gcggtcagcc gttcgagcgt gtccgcgatc
     4201 gaggtataga gattttcctg atccataccc tgcaccagct tgatctcctg ctcctgccgg
     4261 ctttgtttgg cccgtgcgct gtcgggtatg ggcgcggcct cgaaaatggc ccggtagaca
     4321 ttgttatccc ttttttccag ctcgcccatc tgctcctgca gcttggtcac cttatcgttg
     4381 agggtataat agttgtcctg cagggcgtcg ttccgctgca tgagacgttt ttcgtttgcc
     4441 gaagggaagt atttataagc gatggaaacc aggatcaggg ccgtcaccac cgcggcggac
     4501 aggaaaccca aaacgcgcaa aagggtcacc cgcagaggcg tctccagctt ttcatagcgg
     4561 agggtattgg tattatagta gtacttgatc ttcttcatcg gcccgctcgt ctatcatcca
     4621 atagggttag aacggcaaaa cagcctgatt tggtgtacct ttgcacggcc taaactgctt
     4681 ccggcagtcg ttcccttagg accaacatta gcgcctggac cggggcacac ccgcgcaaat
     4741 atagttgctg cgggccaaaa aacaaccaaa agataccgcc gatttatgat gacgagttcc
     4801 gaaataagaa agaaattcct ggaatttttt gtatcgaaag gacatactat cgtgccttcc
     4861 gcctctatcg tcgtgaaaaa tgatcctacc ctcaaattca ccaatgcggg gatgaaccaa
     4921 ttcaaagact atttcctcgg caacaggaag gcgccttatt cccgcgtggc cgatacccag
     4981 aaatgtcttc gcgtcagcgg gaaacacaac gacctcgaag aagtaggcgt agatacctat
     5041 caccatacca tgttcgaaat gctgggtaac tggagctttg gcgatccggc ggacccttcg
     5101 gccggctatt tcaaacccga agccatcgca tggagctggg aactgcttac gaaggtctat
     5161 ggcattcctg tggaccgtct atacgtcacc gtttttgaag gcgaccctga gaacaacctc
     5221 cccgccgata cggaagcgca acacgaatgg aagaaatgga tcgcccccga acgtatcctt
     5281 ctcggaagca aaaaggataa tttctgggaa atgggtgata ccggtccgtg cgggccctgt
     5341 accgagatcc atgtcgattg tcgtaccgat gccgaacgtg cagtgaaaga cggcgcctcg
     5401 ctcgtcaata acgaccatcc ccaggtcatc gagatatgga acaatgtatt tatacagttc
     5461 aaccggctaa aggaccgcag ccttcagccg ctgccggcca aacacgtgga taccggcatg
     5521 gggttcgaac ggctggtccg ggtgctccag ggcaaacatt ccaactatga taccgatgtc
     5581 ttcaccggca ccatcgcaat ggtaggaaac atcgtcaaaa aggaatatac cgccggcgac
     5641 tccaaacgcg acgtcgcttt ccgggtcatc gcggatcata tccgcgccat ctcctttacc
     5701 atcgccgacg gtcagttgcc ttcgaatacc gatgccggct atgttatccg gcgtatcctg
     5761 cgacgggccg tgagatacta ttattcctat ctggattata aacaacccct gttgttccag
     5821 ctggtccctc ttctggccga tcagttcagg gacgtatttc ccgagcttca tcagcaggtc
     5881 gatttcatcg cgaaagtgat gaaagaagaa gaagaagcct ttctccgcac gctggaaaag
     5941 gggcttcgcc ggatcgcgga ggtcatttcg gatgccagat cgcgaccctt ctccgtcccc
     6001 gatgcccaca gcaaaacgat ctccactgac aaggcgatca ttcccggcaa tgtcaccttc
     6061 gagcttttcg atacttacgg tttcccgccc gatctgaccg aattgatcgc aggagaaaat
     6121 ggaatgatcg ttgacaaaac gggattcgac gcggagctcc agcagcaaaa gacccgctcg
     6181 cgcgcggcaa cggcgataga taccggcgac tgggtggtcc tcatcgaaga cccccttgcc
     6241 gaattcgtag gttacgatct gttggaatcc aaaacccgca tcgtcaaata ccgtaagata
     6301 aaagccaaag gcaaggaatc ctggcagctg gtgctcgaag ccacgccctt ctatgccgaa
     6361 agcggcggac aggtcggcga taccggtacg ctcacgtccg gcatggaaac cattcccgtg
     6421 ctcgatacga aaaaagagaa cgatctcatc gtccacttca cagataaggt cccttccgaa
     6481 cccgaggcgc ccgtcattgc cagggtcgat agggtcaaac gtaaggccac cgagatccat
     6541 cactccgcca cccatctcct gcacgcagcg cttcgcaagg tgttggggac gcatgtggcg
     6601 caaaagggtt cgctggtcaa cgatacacag ctgaggttcg acttctcgca tttcgctaag
     6661 atgaccgatg acgagctatc gactgtcgaa tctctcgtca atgaaaaggt gcgcgaaaac
     6721 atacccgtcg tcatccggct gatgcctaag gaggtagccc ttctctccgg cgccatggcg
     6781 ttattcggcg aaaaatatgg cgataccgtt cgggtcgtca ccatcgatcc cggctattcc
     6841 atcgagctct gcggcggtac acacgtcggc gccaccggcg agctggggtt cttccgcatc
     6901 cgtcatgaag gtgccgtcgc tgccggagta cgccgtatcg aagccgtcgg aggcagggct
     6961 gcagaaacgt ttgtcggcga acagctggcc gagctgcagg ccgtccgcga ggtattaaaa
     7021 aatcctgccg atgtctcccg cgcagccgaa acgctcgcgt ccgaaaatgc cgatctgaaa
     7081 aagaaactcg agaaagccga agccaaacag ctcgacatcc tccggcaacg gctattgcag
     7141 accgtacaga tcgtcggggg tgtagccttt atcggggaga tcgtggaagt ccctggcgcc
     7201 gattcgctaa aaaagctatg tttcgatctg aagcccggac tggccgccct cccgcaatct
     7261 acaccctatc ttgctatcct cgccgccaac atcgacggca aagccgccat tgccctcctc
     7321 ctcgatgaac agctggtctc cgcgcgcaac ctgcaggctc ccgccatcat caaggaacac
     7381 gtcgcacccc tgatcaaagg cggaggcggc ggccaaaaaa cgctggctac ggcaggcgga
     7441 caggatgcaa gcaacctggc gcaggtgatc gggaaggtta ggagcctaat ttaacatttc
     7501 cgaaaacttt cgtatttccg gaaatccccc tatctttatc tacgaaaacc aacgtagaca
     7561 gaaaaatttc ctaatcttaa atcccaaaag gatgaataca cacctccaaa aaatggccgg
     7621 agtagccatg ctggcgctgg ctttgaacac caccggtctt gcacaggaca tcactcctcc
     7681 tccgacgccg tcgggagccg acatggacat cgacaccgca gtcaatccca aacacggcta
     7741 tgacgagatc atcatccggc gcaaaacgga taaagacgcc aaagtgacca tcgaaataaa
     7801 gaacggtgag acctatatca atggaaaacc tgcttcggaa taccaggacg gcgatgtgtc
     7861 catcagcagg aggaagatca ggacaatgag agccccttat atcgtgatgg atggcaatga
     7921 tgacatggtg atcaagcctt ccccgttccg taattcctgg agcttcgggg gctccaa
//
LOCUS       DASYSV010000345         4696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_707534, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000345 DASYSV010000000
VERSION     DASYSV010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707534"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VGQ51_10325"
     CDS             <1..979
                     /locus_tag="VGQ51_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEV9037010.1"
                     /translation="FSSNSSTIPWAMKTGKLTLQPFSVVHSILYDETKGKASGVLVVD
                     AHTKTTTEYHARIIFVNAACLNTNLILLNSKSRRFPNGFGNDNGLLGHYIAFHNYRGT
                     VYGVFDGYPDGYYYGRRPTEVFMPNFRNLRRDEGLGFRRGYMSYFSASRPGWVQGKGR
                     DGIGADFKGSITEPGPWWVGMGMQGETIPKFENHVRLSPDLKDEWGIPQLITSIAYDD
                     NDDRMAADFMARGSEMLEAGGCKEVRSNNTRQNPGLDIHEVGGVRMGKDPKTSLLNEW
                     NQLHTCRNVFVTDGACMTSTGNQNPSLTFMALTARAANHAVDEMKRGNL"
     gene            976..1707
                     /locus_tag="VGQ51_10330"
     CDS             976..1707
                     /locus_tag="VGQ51_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV9037011.1"
                     /translation="MITLSIVLIAFLATLVRSTFGFGESMVAVPLFALLIPLDAAVPL
                     AVLMSVLVALVIVLQDRQHIQFKSARGLILFAVLGIPFGLALLAYGNEYWVKIFLGLL
                     IIGNSIYALQRKSNRHKDHDSRWWLFICGFLSGVLGGAYGLNGPPLVVYGNMRQWSPA
                     QFRATLQAYFLPVSFLGLIGFGTQGMLNRAVVNDFLYCLPAVFPAIFAGRYLNRRLRG
                     NAFFKYIYWGLIVIGGILLLHNRNP"
     gene            complement(1650..2480)
                     /locus_tag="VGQ51_10335"
     CDS             complement(1650..2480)
                     /locus_tag="VGQ51_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEV9037012.1"
                     /translation="MKRQFILLIPVILLAASSCSRQAKEDLVFVKGGAFRKTEMPDFY
                     IGKCEVTQKEWTGVVGNNPSTFKGDELPVETVSWYDCVDYCNKRSLREGLKPYYHIEK
                     DKKDTANTNPLDSVRWTVTVNHDADGYRLPTEAEWEYAARGGQLSKGYAYSGSNNLEE
                     VGWYWRNSGDNYLTGGWSWPALAQNNNKTHPVGSKAPNELGLSDMSGNVREWCWESRA
                     GDTTGEAQGRIWKGGGWMGADFCCEPSFHALHQANGKGPDQGFRLCRSNIPPITIKPQ
                     "
     gene            complement(2484..3212)
                     /locus_tag="VGQ51_10340"
     CDS             complement(2484..3212)
                     /locus_tag="VGQ51_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HEV9037013.1"
                     /translation="MKPNTILQQVAGKVTLGTALLLFVTGSSILTGCKKNSSPSSKPA
                     GTRQTGYVLDNTQPTGTGTSNGFFWSLYDEGGTSSITQGSAGNFAISYSNVTDVVGGK
                     GWNPGSARTIGYNVGALSGSYNFVGIYGWTTSPLIEYYVAELGNVSGGTQVNTVSSDG
                     HTYSFYKFQRVNEPSIIGTATFWQYKDNWGGSSTKANHSVTMANHINNWRSHGGQGFG
                     SYNYQILALEAYGGKSGYINATAW"
     gene            3532..4482
                     /locus_tag="VGQ51_10345"
     CDS             3532..4482
                     /locus_tag="VGQ51_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037014.1"
                     /translation="MGPLLAEILPGATRFSSIFVLPIEMCVWGICALLIREALRRFGL
                     GWTSLVLLGLALAVAEECLIQQTSLAPMVIHLKGLTYARAFGVNYVYFLWALIYEPVF
                     VVFLSIAIVEMIYPDRRQLPWITKPGWFVLIPLLLLGSLLAWFSWTQIARVKIFHLEA
                     YRPPVSAILLSVAGMGLLIVAAFRIKQPRVKPMTPPGPVALGIVGGVWAAILFGLVLL
                     GFGADPAFPEWIAVTVPIVLLILIPILILPRYTSHPGWNIQHQFGLSCGTIIGSMAAG
                     QIGFIGTYGADLYFKIISNIIAVILLILLGIRINKQKAMA"
     gene            4482..>4696
                     /locus_tag="VGQ51_10350"
     CDS             4482..>4696
                     /locus_tag="VGQ51_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037015.1"
                     /translation="MPKEVKPPKEVFDLMWEIRIFTRSSLMLQQNISQEAGLNATDAE
                     CLDYLMEMGPSTAGALAQATGLTTGAVT"
BASE COUNT          986 a         1306 c         1237 g         1167 t
ORIGIN      
        1 tttcagcagc aattcctcca cgatcccctg ggcgatgaag accggcaagc tgacccttca
       61 gccctttagc gtcgtgcatt cgatcctcta tgacgagacc aaaggcaagg caagcggggt
      121 gctggtggtg gacgcgcaca ccaaaacgac gacggaatac catgcccgca tcatcttcgt
      181 caatgccgcc tgtctcaata ccaacctcat cctgctcaac tcgaagagcc gccgttttcc
      241 aaacggtttt ggcaacgaca atggcctgct gggacattac atcgcctttc ataactaccg
      301 tggaacagta tacggtgttt tcgacggcta tcccgacggt tactattacg gccggcggcc
      361 caccgaggta ttcatgccca acttccgcaa cctccgacgt gatgagggcc tcggtttccg
      421 gcggggatat atgagctatt tcagcgcatc gcggccgggc tgggtgcagg gtaaaggccg
      481 ggacggcatc ggcgcggatt tcaagggctc gattacggag ccaggtccct ggtgggtggg
      541 gatgggaatg cagggcgaga ccattcccaa gttcgagaac catgttcgtc tcagtcccga
      601 tctgaaagac gaatggggca tcccgcagct gatcacctcc atcgcctatg atgataacga
      661 tgaccgcatg gccgcggatt ttatggcgcg gggcagcgag atgctcgagg ccggcggctg
      721 taaggaggtg aggagcaata acacccggca aaaccccgga ttggatatcc atgaagtagg
      781 tggcgtgcgg atgggtaagg acccgaagac ctcgctgctc aacgaatgga accagctcca
      841 tacctgcagg aatgtgttcg tcaccgacgg cgcctgtatg acatcgaccg gcaatcagaa
      901 tccatcgctg accttcatgg cgctgaccgc gcgcgccgcg aatcatgcgg tggatgagat
      961 gaagaggggc aatttatgat cactctttca atcgtcctga tcgcttttct cgctaccctg
     1021 gttcgctcaa ccttcggttt tggagagtcg atggtagccg taccgctgtt cgccttgctc
     1081 atccccctcg atgcagcggt cccgctggcg gtgttgatgt ccgtgctggt agccctggtg
     1141 atagtgctgc aggatcgcca gcatatccag ttcaaaagcg ccagggggct catcctcttc
     1201 gccgtgttgg ggatcccttt cggtttggcg ctgctcgcct atggtaatga atactgggta
     1261 aagatctttt tggggttgct gatcatcggg aacagtatat acgccttgca aaggaaaagc
     1321 aatcggcata aggatcatga cagtcgctgg tggttgttca tttgcgggtt cctatcgggt
     1381 gtactgggag gtgcgtatgg tctcaacggc ccgcccctgg tggtgtatgg gaatatgcgc
     1441 caatggagtc ccgcccagtt cagggccacg ctgcaggcct attttttacc ggtgagtttt
     1501 ttgggattga taggctttgg cacacaagga atgttgaacc gggcggtagt gaacgacttc
     1561 ctttattgct tacccgccgt ttttccggcg atttttgcgg gaagatatct caaccggcgg
     1621 ttgcggggga atgccttctt caaatatatt tattggggct tgatcgtgat cggtggaata
     1681 ttgctcctgc ataaccgaaa cccctgatcg ggacctttac cattcgcctg gtgcaatgcg
     1741 tggaaagacg gctcgcagca gaaatcggct cccatccatc ccccaccctt ccagatccgg
     1801 ccctgtgcct cgccggtagt atcaccggca cgcgattccc aacaccactc ccggacattt
     1861 cccgacatgt cggaaagtcc cagttcgttg ggcgccttgc ttccaaccgg atgagtcttg
     1921 ttattgtttt gcgccagcgc cggccaggac cagccccctg tcagataatt gtccccggag
     1981 ttcctccagt accatccgac ttcttcgagg ttattgctgc cgctgtaagc ataccccttg
     2041 ctcagctgtc ctccgcgtgc ggcatactcc cactccgcct ccgtcggcaa ccggtagccg
     2101 tcggcatcgt ggttgaccgt gaccgtccac ctgaccgaat cgagagggtt cgtattagcc
     2161 gtgtcttttt tatctttttc gatgtgataa taaggtttca atccttcccg gaggctcctt
     2221 ttgttacaat aatcgacgca gtcgtaccag ctgaccgttt ccacgggcag ttcatcgcct
     2281 ttgaaggtgg aaggattatt tccaacgacc ccggtccatt ctttctgtgt gacctcacac
     2341 tttccgatgt aaaagtcggg catctccgtc ttgcggaaag ctccgccttt gacgaataca
     2401 agatcctcct tcgcctgccg cgagcacgag ctcgcggcaa gcaggatcac cggtatcagg
     2461 aggatgaatt gtcttttcaa gtcttaccag gctgtagcgt tgatgtatcc gctctttccg
     2521 ccatatgctt ccagcgcgag tatctggtag ttgtacgaac cgaaaccctg ccccccgtga
     2581 ctcctccaat tgttgatgtg attggccatg gtgaccgagt ggttcgcctt ggtggacgat
     2641 ccgccccagt tgtctttata ttgccagaac gtagccgtgc cgatgatcga aggttcgttc
     2701 acacgctgaa atttataaaa gctgtacgta tgtccgtcgc tgctgaccgt gttgacctgt
     2761 gttccgccgg atacattgcc cagctcggcg acataatatt cgatcagcgg gctggtcgtc
     2821 caaccataga tgccgacgaa attatagctg ccgctcaatg cgcctacgtt atagccgatg
     2881 gttcttgcgg agcccgggtt ccagcctttg cccccgacta catcggtgac attgctgtag
     2941 ctgatggcaa aattacccgc gctgccctgc gtgatgctgg atgtccctcc ctcatcatac
     3001 aagctccaga agaagccgtt gctggtaccc gtgcctgtcg gttgggtatt gtcgagaacg
     3061 tagcctgttt ggcgtgtccc cgcgggtttt gaagacggtg agctgttttt tttgcatccg
     3121 gtcaggatag acgagccggt gacgaataaa agaagtgctg tgcccagggt aaccttcccg
     3181 gccacctgct ggaggatcgt gtttggtttc ataaatggca aattttgtta gtgtatctaa
     3241 atttagtgaa gttggatgaa aagttgcaac cgattgcacc caactacagg ataacctgta
     3301 taaagtgcag taactcagca gcgcaaccga ttacgtagaa ggtatcaaat cggcaccgat
     3361 tcggacaatt tcaacgccta ttcggacaat ttcgacaccg atttggagat ttcaaaagaa
     3421 gtttctacat ttaccttaat cattaagata cttataaatt aagataaaat gaacgcctca
     3481 acctcacctt cgaaaaaagg ggtttttgca ccggcaataa tcctgatgct catgggccca
     3541 ctcttagcag agatcctccc cggagcgacc cgtttcagtt cgatcttcgt cctgccgatc
     3601 gaaatgtgcg tatggggcat ctgcgccctc ctgatccgtg aagcgctgag acgattcgga
     3661 ctcggctgga caagcctcgt acttttggga ttggcgctgg ccgtcgccga agaatgcctc
     3721 attcagcaga cctctctggc ccccatggtc atccatctca aaggtctcac ctatgccaga
     3781 gcgttcggcg tcaactatgt ctatttctta tgggcgctca tctatgaacc cgttttcgtg
     3841 gtctttctct ctatcgccat cgtcgagatg atctatcccg accgccggca attgccatgg
     3901 atcaccaaac ccgggtggtt cgttcttatc cccctattgc tgctgggatc gttgttggcc
     3961 tggttctcct ggacacagat cgcgcgagtc aagatcttcc atctcgaagc ctaccgacca
     4021 cctgtatccg ccatactctt atctgtcgcc gggatggggt tgctcatcgt ggcggccttc
     4081 aggatcaaac aaccccgggt aaaacctatg acaccaccag gcccggttgc attgggcatc
     4141 gtcgggggag tatgggccgc tatcctcttc ggtctcgtct tactgggctt cggcgcggac
     4201 ccggcctttc ccgaatggat cgcggttacg gtccccatcg tgctgctcat tttaatcccc
     4261 atcctcattt tgccccgtta taccagccat ccgggatgga acatccaaca ccaattcgga
     4321 ttatcttgcg gtacgatcat cggttccatg gcagcgggtc agatcggatt tatcggcacg
     4381 tatggcgcgg acctttattt caagatcatt tccaatatca tcgcggtgat cctgctgatc
     4441 ctgctgggta ttcgtataaa caaacaaaaa gcaatggcct gatgccaaaa gaagtgaaac
     4501 cacccaaaga agtattcgac ctgatgtggg agatccgaat atttacgcga agctcgctga
     4561 tgctgcaaca aaacatatcg caggaggccg gtctcaacgc aactgacgcg gagtgtctcg
     4621 actatctgat ggaaatgggt ccgtccaccg cgggggccct ggcacaagca acggggctca
     4681 ccacaggcgc ggtgac
//
LOCUS       DASYSV010000346         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_67749, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000346 DASYSV010000000
VERSION     DASYSV010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67749"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VGQ51_10355"
     CDS             complement(<1..187)
                     /locus_tag="VGQ51_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV9037016.1"
                     /translation="MAIDTHNPEFQLAAQFVHQTSRSLFLTGRAGTGKTTFLRHIRDT
                     GRKKMAVLAPTGVAAINA"
     gene            complement(233..1783)
                     /locus_tag="VGQ51_10360"
     CDS             complement(233..1783)
                     /locus_tag="VGQ51_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV9037017.1"
                     /translation="MNPKLTTLLFLLSLAGRGLGQVQEPVISNIVKEETDHSQLRQLA
                     HELFDRIGPRLVGTPEMDSANAWAVEKYTGWGIPAHNEKWGEWRGWERGISHIDMVSP
                     RVKSLEATQLAWSPTMGNKTVTAELVILPDLQDSVAFTQWLPAVKGKFVMISMLQPTG
                     RPDYNWEEFATKESFEKMKKEREAEREAWALRIRKTGYTNRTLPVALEKAGAAGVVIN
                     NWSNGFGVDKIFGAYTKKIPTIDIGLEDYGMLYRLTESGDKPRISVHTESKERGVVPT
                     FNTIAEIKGSEKPDEYVILSAHFDSWDGGTGATDNGTGTLTMMEAMRILHLVYPHPKR
                     TILVGHWGSEEEGLNGSRAFVEDHPEIVAHIQAVFNQDNGTGRVVNLSGQGFLNSYDY
                     LQRWLSRVPSNIRDSIKTTFPGIPGGGGSDFASFVAMGAPAFSLSSLNWSYGAYTWHT
                     NRDTYDKIVFDDLQNNAILVAALAYMASEDPVTTSREKSVLPVNNRNGQQIKWPEPVK
                     SIRKGGLD"
     gene            1827..>2840
                     /locus_tag="VGQ51_10365"
     CDS             1827..>2840
                     /locus_tag="VGQ51_10365"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEV9037018.1"
                     /translation="MRTLFLSFLIVITVTNIRAEVRLPSVLSGNMVLQQQSRASLWGW
                     CEPGEKIMVTVGWDGQVDSVKGDRDGRWKLQVTTPSAGGPYNITIKGGNTIVLENILI
                     GEVWVCSGQSNMEMCETWGMPDVRAELPSCYNPNIRMFHVPRTTAEAPQDNCSGKWTI
                     CDSNSLKQFSAAAYFFAKRLNKDLNVPIGLIEAAWGGTCAEVWTPAHLIEGDEILKTA
                     AGKQKPYDFWPYKPGYCYNAMIAPLTSFAIAGAIWYQGEANVGTPYTYQRVMTTLIAS
                     WRQAWGLPLSFYYVQIAPFTYGTKDQAAVLREQQGRIQQVENTGMVVISDLVDDTTNI
                     HPKN"
BASE COUNT          589 a          741 c          843 g          667 t
ORIGIN      
        1 ccgcattgat ggctgccacg ccggttggcg caagcaccgc cattttcttt ctgccggtgt
       61 cccggatatg acgcaggaaa gtggtctttc ctgtgccggc tctgccggtg aggaagaggg
      121 atcgggaagt ttgatggacg aattgcgccg ccagctgaaa ttccggattg tgggtgtcga
      181 tagccatagc aaggggcaaa tttactatgg tccatcgaac ctcgcagaaa atttagtcca
      241 gtcctccttt ccggatcgat ttcaccggct cgggccattt tatctgctga ccgttgcggt
      301 tgttgacggg caaaacgctc ttttcacgcg aggtggtaac ggggtcttcg ctggccatgt
      361 atgccagcgc cgcaactaaa atggcattgt tttgcaggtc gtcgaagact atcttatcgt
      421 aggtgtcgcg attggtatgc caggtatagg cgccatagga ccagttgagg ctgctgagtg
      481 aaaaggcggg cgcccccatc gctacgaacg aggcgaaatc cgagccgcct ccgccgggga
      541 taccggggaa ggtggttttg atgctatcgc ggatattgga aggaaccctg gacaaccagc
      601 gttggagata gtcataagaa ttcagaaaac cctgaccgga cagattcact acccttcccg
      661 tgccattgtc ctggttgaac accgcctgga tatgcgcgac gatctccgga tgatcttcca
      721 cgaatgctcg cgagccgttg agtccttctt cctcactgcc ccaatgtccg actaaaatgg
      781 tgcgtttggg atgcggatag acgaggtgaa ggatacgcat cgcttccatc atggtcagtg
      841 ttccggtacc gttgtccgtc gctcccgtgc cgccgtccca actgtcgaaa tgggcggaga
      901 ggattacgta ttcatcgggt ttctcactgc ctttgatctc ggcgatggta ttgaaagtgg
      961 gtaccactcc gcgttctttc gattccgtat gcacgctgat gcggggtttg tcgcccgatt
     1021 ccgtcagccg gtaaagcatc ccatagtcct cgaggccgat atcgatggtc ggtatctttt
     1081 tggtgtaggc tccgaatatc ttgtctaccc cgaaaccatt ggaccagttg ttgatgacga
     1141 cccctgccgc cccggctttt tcgagcgcca cgggcagggt gcggttagtg tatcccgttt
     1201 tcctgatccg gagggcccag gcctcgcgtt ccgcctctct ttcttttttc attttctcga
     1261 aggattcttt tgtggcgaac tcttcccagt tgtaatcggg gcggcccgtg ggctggagca
     1321 tggagatcat aacgaacttg cctttgacgg ccggcagcca ctgggtgaag gcgacggagt
     1381 cctgcaggtc gggcaggatg accagctcgg ccgtcacggt cttattgccc atcgtagggc
     1441 tccatgccag ctgggtggct tcgagtgatt tgacccttgg tgaaaccata tcgatgtgcg
     1501 agataccccg ttcccatccg cgccattctc cccatttctc attgtgcgca gggatacccc
     1561 agccggtata tttttccacg gcccatgcgt tggccgaatc catttccggc gtacccacaa
     1621 gccggggacc tatgcggtcg aagagctcgt gcgccagctg tcgcagctgg gagtggtccg
     1681 tttcctcttt gacgatgttc gatataacgg gttcctgtac ctggcccagg ccccggccgg
     1741 ccagcgagag caggaaaaga agggtagtta atttcgggtt catgaaggta atttaaggaa
     1801 aaaaatagaa ttcgtaaatt aggtgcatga gaaccctttt tctgtcattc ctgatcgtta
     1861 ttacggtcac gaacatacgc gccgaagtgc ggctgccctc agtattatca ggcaacatgg
     1921 tcttgcagca gcaaagtcgt gccagcctgt ggggatggtg cgagccgggg gagaagataa
     1981 tggtcaccgt aggctgggac gggcaggtgg attcggtgaa aggcgaccgg gacggtcgct
     2041 ggaaattgca ggtcaccact ccgtcggcgg gcgggcctta taacatcacc atcaaaggag
     2101 ggaatacgat cgtgctggag aatatactca tcggcgaggt gtgggtctgc tcgggtcagt
     2161 ccaatatgga aatgtgcgag acctggggga tgccggatgt ccgggcggag ctgccttcct
     2221 gttacaatcc gaatatacgc atgttccatg tgccgcggac gacggcggag gcgccgcagg
     2281 acaactgctc agggaaatgg accatctgtg actccaactc gctgaaacaa ttcagcgcgg
     2341 cggcctattt ctttgcaaag cggttgaata aggacttgaa tgtacccatc ggactcatcg
     2401 aagcggcctg gggcggaacg tgcgccgaag tgtggacgcc ggcacatctc atcgaaggcg
     2461 acgagatact gaagacggct gccggcaaac aaaagccgta tgatttctgg ccgtacaaac
     2521 ccggttattg ttataatgcc atgatcgcgc cgctcacgtc attcgccata gcgggggcca
     2581 tatggtacca gggcgaggcc aatgtgggga caccgtatac ctaccagcgg gtgatgacta
     2641 ctctgatcgc ttcctggcgc caggcctggg gtttgccatt gtctttttat tacgttcaga
     2701 tcgcgccgtt cacttatggg acaaaagatc aggcggcggt gctccgggag cagcaggggc
     2761 ggatacagca agtggagaat accgggatgg tggttatcag cgacctggtg gacgacacga
     2821 cgaatattca tcctaaaaat
//
LOCUS       DASYSV010000347        30068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1475508, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000347 DASYSV010000000
VERSION     DASYSV010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 30068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30068
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475508"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VGQ51_10370"
     CDS             <1..1533
                     /locus_tag="VGQ51_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEV9037019.1"
                     /translation="ILLLLFIFQRFGTKVVGGSFGPIMMVWFLMLGYAGLRQIVHYPA
                     IFASLNPVYGLKMLINHPRGFWLLGSVFLCTTGAEALYSDLGHVGRKNIYFSWTFVKI
                     TLVLNYLGQGAWALMRNKPDLEGLNPFFAIVPSQFLLVSIIVSTAASIIASQALISGS
                     FTLISEAISLGFWPRVKVKFPTDIRGQIYIPSINWILFLGCVLVALYFRTSEAMTAAY
                     GFSITVAMLMTTMLVTYFLRYVKKYPFWLVGAIVTIFLMVESCFFVANAVKLVKRLFF
                     LVFEFGLITTMYVWYNARKITLRLLTFTDVAPILPRLALLSSDTSIPKYSIHLVYLTR
                     ASRHEIENRNVDSIFDNPRKRADFYWFVNIERQDDPYTQEYDVDILIPQKVIRVNLRL
                     GFRIQPLVGVMLRKVAEEMKAAGEMPMVGKPKDFKFVILERFLSYDNEFSVRSGFILN
                     TYFSILKLARSDSRAYGIDSDQAVIEKVPLVVAPVQSITLRRVTKVKERGHGDQESGM
                     PF"
     gene            1632..2051
                     /locus_tag="VGQ51_10375"
     CDS             1632..2051
                     /locus_tag="VGQ51_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037020.1"
                     /translation="MIMKAYTVFDNGGKTVDRFTVINMKTADVFAVSDNPGTPDGIGR
                     HCGNCAEHRIVLYGSGWRQRLPHKKVIEAEAENYINNARLDPDWLGREIEFAALPAGV
                     RSCIVDLEKDVDPGYYSRAKIASLGGFPRQSKSINSK"
     gene            2219..3907
                     /locus_tag="VGQ51_10380"
     CDS             2219..3907
                     /locus_tag="VGQ51_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV9037021.1"
                     /translation="MPQDVPHLSTKATAQNTYDAIVVGSGISGGWAAKELCELGLKVL
                     MLERGKEVHHPNYPTATLAPWDFPHRESLDRADRERSPIQARHFSYREDNKHFYIDDR
                     ENPYEEVKRFDWIRGDVVGGRSLLWGRQCYRMSDLDFEANVRQGVGVDWPIRYRDIAP
                     WYDHVEKFIGVSGNADGVPHLPDGHYQPPMAFNCVEEYFTTRIAAKYADRRVIMGRTA
                     NLTQPVQGRGQCQFRNLCHRGCPYGAYFSTNASTLPAAFATGRLTLRSGSLVNKVLYD
                     NDAQRATGVEVIDRETGKTVEYYARLIFLNAATLATTFILLNSRSNRWPEGLGNSSGQ
                     IGRNLMDHHKSNSITGIMPGFEDKYYYGRRPTGLYIPRWQNFSGEHRDYIRGYGYEGS
                     AYRAGAHPDVSIGAALKEAATEPGPWTISFKGYGECLPYADNRVALNTDKKDKWGRNT
                     LSVDAEFRENEKAMNKDMIVAMQEMLDAAGCKDISAGAGISFPGNANHEMGTARMGRD
                     QKTSVLNGFNQMHEVHNVFITDGSFMTSSGPANPSLTYMAMTARAVHYAVAQLK"
     gene            3999..4193
                     /locus_tag="VGQ51_10385"
     CDS             3999..4193
                     /locus_tag="VGQ51_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037022.1"
                     /translation="MRRKIVGIILSILGIAGLVSAFIYMNGPDSSEHLAALLARGVLG
                     AIAFFAGIWLVDHNAGRRAV"
     gene            complement(4219..5202)
                     /locus_tag="VGQ51_10390"
     CDS             complement(4219..5202)
                     /locus_tag="VGQ51_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEV9037023.1"
                     /translation="MPKLIPASVWSALSLSIAKGDTTGVQHLVEDYAIDVNAFTDSRS
                     WMPVLMEALLSYGFESEEDRLPMLRYLLEKGANPNICSHRGYNCLHIAVQQERYVKAL
                     DLFLDFNPDVNIGDEDGSNVIYWAVQGWLLRKNDENRAEQLRVFGKIIRRGADLDQVN
                     RYGMNARKWLELAAPDVKEMVASWEAGRPAIRAISTVQPAISPLRDPELAYKLWSDLV
                     PASGPAATLQGELLRSVERLRDEAQQNGNANYRKDHRRMAVFVRDTLVRSGIFDKGEV
                     DLIRNNTHMLMKASRPYIKNDVFDHLVDQVCVFYTRNPNPIPYKARRPALL"
     gene            complement(5273..6037)
                     /locus_tag="VGQ51_10395"
     CDS             complement(5273..6037)
                     /locus_tag="VGQ51_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9037024.1"
                     /translation="MIKTILIDDEARLLGSLQTMLHRNCPQLNVIAVCRSADEALVKI
                     SENSPDLVFLDIAMPGKDGFALLEEIGDIRFRIIFVSAHDEYSLRAFKFSAIDYLLKP
                     VDENELVIAVKRAEQTILPERNLQKSIDTLMHNLKPQANSQEKKLCLTTLTGFHVISL
                     SEIVYCEAAGPYTDFYLSAGKKICVSRPLADYESLLGEDGFLRVHKSFLINLHHTREY
                     RRGEGGTVIMSNGAEIDVSRRKKEIFLEKIKGSFKY"
     gene            complement(6034..8199)
                     /locus_tag="VGQ51_10400"
     CDS             complement(6034..8199)
                     /locus_tag="VGQ51_10400"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV9037025.1"
                     /translation="MDELDSLLRLDKAQTAEDEEAVNRWVKIADDYQYRNIAAGLGFA
                     DKAIVLARRLHVRDQLASALEAKGKLYHRSGQYKEAIASLGQALHIFEATGNTKGMSR
                     TYSDFGMVFWQTADWKKAIWYFDRSIAIAANANDEQSIGDTYGDMGIVYYEQTDFPKA
                     LDYFQKALTIHQKYKDTRGIAQNLQDIGMIYSDRSEYLKAMDYLLDAARMNGAIKDLS
                     GLAHNYANLGWLYEEIEDYDRASGYYEKSIAICRQTGEKFGLYFNLQSLGTVNLKIRR
                     YSAAMVDFRQALALADTLGNKHGIAMVLNYMGSIDSELGDEDAAMTAYGKAALLLDSI
                     GNKRDIADNLQKRAALILDAPASALLRHLIPPTARLDTVQLLLRRAKPLAEEAGDKKV
                     KRDILKTSSSLQEKRGNIRKAFSEYRQYVAIRDSLTDKEREKALERKGIQYEFDRKAD
                     SLRFQNQLTETRLGEDMKQERLYGIGVFILLCAATGFLLYYSKIKRVALSAQLERKTS
                     DAILSSLLAQMNPHFIFNCLGSIKQMILENEQENANDYLNKFSKMIRLSLEYSRRPYV
                     TIREDLDYLHNYLRMELVRFDHSFTYHIEVEEGICAEEIGVPPMMVQPLAENAIWHGL
                     MNKSGDRKLFIRYKPGHNQLICEIEDNGIGISHSKLLPRHGHQSFGIGNIRHRLTLLN
                     EKYHLDCNLELKDKSELDTNETGTLAVLTLPILNDPGDI"
     gene            8485..9216
                     /locus_tag="VGQ51_10405"
     CDS             8485..9216
                     /locus_tag="VGQ51_10405"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEV9037026.1"
                     /translation="MKKIMFLLYGVVCYAIFFVTFLYAIGFVNNLYVPKSVETGYQGG
                     TSYAWMVDILLLSLFALQHSIMARKGFKKWWTKIIPEPIERSTYVLFASASLILLFAY
                     WRPLPGVIWDVRNNLLSGLLTGLYFFGWFIVLFGTFLINHFNLFGLQQVYNNLNGSEP
                     ATSRFVTPMLYKFVRHPIMLGFIIAFWSTVRMTSGHLLFSVATTAYILIAIRLEERDM
                     VAMYGADYKRYQEEVSQIVPIPRRK"
     gene            complement(9279..11468)
                     /locus_tag="VGQ51_10410"
     CDS             complement(9279..11468)
                     /locus_tag="VGQ51_10410"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV9037027.1"
                     /translation="MRWKNLLFNITLAINCLLLFLLLFESRVVVPTWLQVAGRMHPLI
                     LHFPIALVVLFVFQRLVLRRADNTLLLLAAFTAALTALMGLFLSKEPGYDPDVLFWHK
                     YSGVAVSFLTLAWYHWYQRLQTVRFAGVGVAIGCLALLTFTGDKGADITHGHNFLLAP
                     VSSASSAGKVSLEDAVVFNDMVRPILQNKCMGCHNSSKAKGQLIMETTQLLLKGGKNG
                     PVWDSSAADFGLMMHRVHLPPDDEKHMPPKGKPQLTDQEITILYQWLRHDPTRTIRVA
                     QLPSNDTLYQLAAAMFKNAQDEDRYDFPSADENTVQKLNTSYRVISPVALNSPGLNVD
                     FYSPQFFRSGQLNDLEPIARQIVSLDLDKMPVTDADVPAIVRFSNLRTLNLSFTAVTG
                     AGIAQLCRLAQLKSLSLSGTPVKAEDISCLLTMKSLRHLYLWNTAIAPGSIASASPFA
                     SAASASASASPSSAASAFASSGKGNNGLDIQMGVRTDTMLIRLNPPILQNDERILLQP
                     MQLRLKHYVPGVAIRYTIDGTSPDTGSTLYSGGTLFSANAVLKVRAFKKGWLPSDSIE
                     TRFYTDRHHPDSVIMLKPLDSNYKGKGPRTVIDLDKGDLNWASGKWLGFRRNPMECVL
                     LFDKPVEAHEVTLSSMINTGASIMPPAGIEVWGGANAHDLRLLGRLTPDTAKPSTAPG
                     LPGYDIGFRPVKLRCLRIVMTPVAKLPDWQIEKGKPGWAFVDEIFVN"
     gene            complement(11468..12490)
                     /locus_tag="VGQ51_10415"
     CDS             complement(11468..12490)
                     /locus_tag="VGQ51_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEV9037028.1"
                     /translation="MQRRQFIQQTTAAAGILFVPPILTADTILGQNHRRYRLNNHWSQ
                     VDFGRYPVKDCHEMVQDRSGRILLLTNDTHNNMLIYDRKGKLLTAWGTEYPGAHGLTL
                     FDENGTEVLFICDNNRHQVIKTTLDGRVLMTLDYPKETGQYTKSEEYIPTETTIAPNG
                     DIYVADGYGKDFVIQYDSKGNYIRHFGGRGDGEEHLLNAHGITIDSRHPGGPFLIVTS
                     RQQNAFKRYTMEGKFLGTIALPGAWVCRPVINGDYLYAAVLQSSSGVGKESGFVTILD
                     KDFKVVSNLGGSEPDYNGGHLGEMRQTVKVFQYPHDVCIDDEQNLYIAQWNSGHVYPY
                     KLEPVS"
     gene            complement(12509..13954)
                     /locus_tag="VGQ51_10420"
     CDS             complement(12509..13954)
                     /locus_tag="VGQ51_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEV9037029.1"
                     /translation="MDKHILEHALTYNRRRFLYRAGLGVGSIALGSLLIPDLFGHSAD
                     EMPQVGLPHFPPRAKRIIYLFQNGAPSQLDLFDHKPLLQQMQGQDLPPSIRLGQRLTT
                     MTSTQTKFPLAGTAFGFNQYGQARVWLSELLPHTARVVDDLCFIRSMYTEAINHDPAL
                     TFMQTGAQVGNRPSMGSWLSYGLGSENKNLPAFCVLLSKGKGNGQGVYSKLWSNGFLD
                     SIHQGVQFSNGENPVLYLGDPDGMTKADRRDMLDRLATLNEESYKEFGDPEISTKIRQ
                     YEMAYRMQMVVPEITDTSKEPEDTLKLYGPDCLVPGTFAANCLLARKLSESGVRFVQL
                     YHQGWDGHANLPMEIKGQCRDVDQASAALITDLKQRGLLDETLVIWGGEFGRTNYCQG
                     TLTKDNYGRDHHPRCFTIWMAGGGVKPGVFGETDDFGYNIIKDPVHVHDFHATALYLM
                     GLDHEKLTFKHQGRRYRLTDVAGKVVHGIIA"
     gene            complement(13965..16295)
                     /locus_tag="VGQ51_10425"
     CDS             complement(13965..16295)
                     /locus_tag="VGQ51_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEV9037030.1"
                     /translation="MVSYNYDIRPILSDKCFKCHGPDANHREAHLRLDIADSAYAPLH
                     VTKGAFAIVPGNPGASMLLKRVSSQDPDFRMPPPDAHLGVLTPHEQKLFTKWIRQGAK
                     YERHWAFTPPVKAPLPTIKDTTRTRNAIDYFVIDRLDKMGLAMNPEADKERLLKRLSL
                     DITGLPPTVAEQDAFLADGAPNAYEKMVDHYLATPQYGEKMAVHWLDVARYADSYGYQ
                     DDNIRTQWPWRDWVISAFNSNMSYDRFITWQIAGDLLPDATKQQILATAFFRNHKYTE
                     EGGVIQEEYRVEYLIDKTKTYGKGILGLSIECAQCHDHKYDPFSQKDYYSLLAFFNNT
                     KEVGYEGDVTKSKPAKTPILLITDSDARLMPFLHKTDTAALMVSVMGERDSLRKTYVL
                     SRGRYDAPSVEVSPSALPAVMPFDTTKYPRNRLGLAEWTVSRQNPLTARVFVNQLWQE
                     FFGRGIVKSSGDFGMQGDLPTDPALLDWLAVDFMDNGWDIKRLVKTFVMSAAYRQSTS
                     VTPEKLEKDPDNAFVSRGPRNRLPAEFVRDLVLSSSGLLVKTIGGPSVKPYQPEGLWE
                     AATSGRGALAAYRQDHHDSLYRRGMYTFIKLTVPPPSMGIFDASNRDQCEVRRLQTNT
                     PLQALVMMNDPTVLEASRVLAQNLLTRRSSTNPSATTTPSATSNPSATTTSSPTTNPS
                     PTTNPSSATNPGSDPLTAAFRTVVCRHPSPKELSVLTDYYHEQLQEFRQKKLDAGKTL
                     AIGEYPENKQLDPNEQAALMKVIELLYNLEETITRS"
     gene            16540..17406
                     /locus_tag="VGQ51_10430"
     CDS             16540..17406
                     /locus_tag="VGQ51_10430"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV9037031.1"
                     /translation="METTQATINYNRIAEAIAFYKANFKDQPGLEKVAEHVHMSPFHF
                     QRMFREWAGVTPKQFLQYLTLEHAKNVLKQPAGATLSDAAYEAGLSGTGRLHDLFIRI
                     EGMTPGEYKNGGEALQIRYSFAESPFGPLMVASTDKGVCYMAFVDNGNEAALRDLKEH
                     FPNAQYEAGADPQQQRALSIFTGDWTSPEALRLHLKGTPFQVKVWETLLKVPAGGLTT
                     YAEVAARLQNPKAVRAVGSAVGQNPVAFLIPCHRVIRSDGQVGEYHWGDTRKNAIIGW
                     EAAKAHTASERA"
     gene            complement(17412..19805)
                     /locus_tag="VGQ51_10435"
     CDS             complement(17412..19805)
                     /locus_tag="VGQ51_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037032.1"
                     /translation="MFKSYFTIAIRTILRHRVFSVINILGLAIGMTAFFLIFQYVRFE
                     TSYDRWHTNADRIYRVVPDISTPTGTDSYQGRCPAPVASRLKKDFPEIEEAVRMSPQE
                     LLVTAGDRRFQEHGVVLTDSGLFHIFDFQLIAGDKNTALTQPMSVVLSESMARKYFGT
                     TDVLGRHLQLTASALTATVTGVIKDIPANSQIRTGIFLSMASNRHIHGDDNPDSPDWT
                     AGFGYFTYILLRPHTAATALEAKLPAWLDRLAGSQLRPLQLRVSFLLEPLTRVYLYSK
                     REAAQHGSIENVRIFSIIGLFILALACINFINLTTARSVDRAKEVGVRKVIGALRSQL
                     SLQFIGESVILCLLAFLLTVILCGVLTPLFDRLAGKTISPGIFSQPTDILLLLGLAIL
                     VGLAAGFYPALVLSSFRPVTVLKGRFSTGARGSLLRKVLVVSQFTVSILLLIGTAVVY
                     AQLSFMRSSQLGFKKEQTLIIPTMSDARAEVFKQSLSEIPAVLGSTYSSSVPGEGLFN
                     AYTELQDRKGEMQKANLDLYTVDYDFVPQYGLELVAGRNFSRSFPSDSTQAMIINESA
                     ARMLGYAKPGEALGRDFAQWGRRGKIIGVLRDFHNLSMKHRIAPLVMRIEPFGYYSIS
                     VKLAGAGLPATLKTIAAEWAQAMPDRPFEYRFLDEIFDEQYRADSRFGRLFVNFAALA
                     IFISCLGLLGLAAYNTLQRTKEIGVRKILGASAATIVKLLSLDFIRLIGIAFLIAAPL
                     AWFLMNRWLEGFAYRTGLSWWIFAGAGAAAVGIAVATISFQSIRAALANPVDSLRTE"
     gene            complement(19814..22231)
                     /locus_tag="VGQ51_10440"
     CDS             complement(19814..22231)
                     /locus_tag="VGQ51_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037033.1"
                     /translation="MIKNFFLLTWRNLIRHKGFSFINITGLAIGMASALLIGLWIKNE
                     LSIDRIYPKTDRLYMLYNRGTFNGAVQVWDQTPKSIGPVLKKDYPGVEDAARYAGDRF
                     LTSVGDIHLNCIAGFADSGFLTMFGLPMLAGDPAHALGDMYNIVLTRQMAVRLFGNRD
                     PMGKTVRLDSNANFTVSGILEDLPATTSFSFDYLVPWAYMTHLGWNDEQNWGNNSIST
                     FVLLKPGVTQASFDKQVADVTRSHSKEQAEVFTQPISRLHLYSKAQDGRLVGDKIVTV
                     RLFSAIAVFILLIACINFMNLSTARSERRAREVGIRKVVGAMRRSLIAQFIGESTLIS
                     SLAFIAAIFLTHISLPAFNRLVGEQLSIDDSSPGFWIFGVAFVIFTGLLAGSYPAFYL
                     SSFRPVTVLKGTPGFRMRLSVSTQLSRNSFRKVIALVNSRKILVVLQFSFAIALVICT
                     TIVFKQLRYGIDRDAGYNRDQLVYFYTQGAAFGHQDAICRELLASGAAISVTNTPGPV
                     TRHWSDTWGFQWRGSTKADGEVDFLTFGAKSNFVQTMGITLLQGRDIDIDKYPGDSSA
                     LLLNESAVKAMRLKNPLGQEIRQGSASPYHVVGVIKDFILESPFTKKIAPMMIAGPGG
                     IYFQVINFRLNPAHTTAANLALAEKVYHRYSPDYPFDYHFVDEAYAEKFKAEQRITRL
                     SALFAALTIVISCLGLFALAAYMAENRIREIGVRKVLGASVAGITTLLVRDFVALVLV
                     AFLISAPVAWLVMDKWLLNYSYRIEMGWDVFAFSGVLAVLIAVATVGYQSVRAAVANP
                     IKALRTE"
     gene            complement(22241..24670)
                     /locus_tag="VGQ51_10445"
     CDS             complement(22241..24670)
                     /locus_tag="VGQ51_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037034.1"
                     /translation="MSISQLPFWHIFYGDYPMLRNYFKIAIRNLLRNKAFSAINIAGL
                     AIGMASALLIGLWIHNELSVDRFFSKKDRVYQIYSREMTNGRLTAWGRAPTPLVPQLK
                     QTYPEVEDAARFRTVYFLLIKDDARFNLEGAFADSGFLSILDFPLLEGNPRTALNGPR
                     DIVLTRHVAKSLFGNEDPMGKTVLIDSNRQFRVTGVLKDLPPNTEFTFQYLIPWAHVD
                     ELGWDPLAVAWQITNAGAYVLLKPGTSATAFDNKIRHIIRDHVKTGQGFDREIFTQPI
                     TREHLYSQVDNGRLVAGRITTVRLFILIAVFILLIACINFMNLSTARSVKRAREVGIR
                     KVAGALRSSLITQFIGESILLAAIAFVLALGIVGLSLPAFDEVIGIPLTLDLTSVGFW
                     IFSAAFIFFTGILAGSYPAFFLSAARPVKVLKSGSMRTANALITPRKVLVVLQFTFAI
                     ILITSTMIVEDQLKYARDRDAGYDRNRLAFVFAQGDVLPHYDAIRHDLLSSGTAVGVT
                     KTFSPMTRVWGETTGYSWSGSTPADKGLYFTQFQADADFVHTTGTHLVEGRDIDLGTH
                     PTDSFAVLLNETAVRTMRLDHPVGALITDDAGVHLHVIGVIKDFIIANPYEPIVPMII
                     QGLSTAYPVIHFRLNPDRPLADVLASAGSIFRRYNPQYPFEINFADDIYNAKFRAEQQ
                     EGTLGALFAALTIFISCLGLFGLAAYMAESRMREIGIRKVLGASVTGITMLISGDFVK
                     LVLVALVLAIPVSWIAMDDWLHGFTYRIHITGTVFLLAGILAIAIALLTVGFQSIRAA
                     LGNPVRALRSE"
     gene            complement(24775..27396)
                     /locus_tag="VGQ51_10450"
     CDS             complement(24775..27396)
                     /locus_tag="VGQ51_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037035.1"
                     /translation="MARKLTGEATPEEIAELELFQQQHPESTYSLQVLADLWRSRQSP
                     EVPDADSAFNRHLTRMALRDLKRIHREPVAAAVKAGPFAQTRDLIDNYFKIAWRSLLR
                     NKGFTAINISGLAIGLASAIVLLLWIRNELSFDQFHTNRDRIYQVMSRGSFDGRMEVN
                     SNTPMVLGPVLQTGYRDEVETVVRLNWVGAFILSAGDKHVGTQGYLTDPGFFRLFSFQ
                     LLEGDTATALNGPRKIVLTRRLAEKLFGNDDPLGRNVRVDSTGDFIVSAVMKDQPPNT
                     AFTFEYLIPWSYTKEVHWEETSWKKSTIATYVLLRPGVTEGDAESRLRTVLHAHQPEL
                     SSLLFLHPMRKWRLYSNFVDGKATGGEINFVRMLAIIAAFILAIACINYMNLSTARSI
                     RRAREVGIRKVVGAGKGSLISRFLGESVLIAALAGILALGIVVVALPWFNRLIETGLS
                     IPYDDPLFWLAGIAFVVFTGLLAGSYPAFYLSAFRPVNILRGHFRTIHALVTPRRILV
                     VFQFTFAIAFIICTIVMYREFYYAGHRDMGYNKKGLAFIYIKGDIRKNYPAIRDELIG
                     SGAVTELTRSNAPFTEIWSSGDGYQWKGKDPKQETWFIEYRTDRGLTSTLDLKVLAGR
                     DIDVEKYPSDSTALLLTSEAAKTMGFANPIGQTVVNNGFTYHVVGVVADFITGTLFNR
                     VLPIVIQGPTSWFGALTFRFNPRNDRARNERTLASIVKKYNPNYPFEYFYVDDFVAAK
                     MQGDKHFGTLAALFAGMAIFISCLGLFGLSAYMAESRLREIGVRKVLGASIMRLTALL
                     SKDFVILVCIAFVIASPVAWWFMRQWLDGIPYHIDLNWWIFPLTGVLSLCIAAGTVGY
                     QAIKAALSSPAMVLRTD"
     gene            complement(27479..27931)
                     /locus_tag="VGQ51_10455"
     CDS             complement(27479..27931)
                     /locus_tag="VGQ51_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV9037036.1"
                     /translation="MNQPLVVKSSISIHAPAAKVWDALTNPEQTKKYMFGCAALSDWK
                     PGSPLIWKGVFNNVEMVAVKGHIVTIEPNRLLVYTVIDPNNAEIPDIPANYLTVTCEF
                     SEEHGRTLLTVSQGDYNTVAQGAVRYEHSVRGGGWDPMLVQIKALVEG"
     gene            complement(27982..28947)
                     /locus_tag="VGQ51_10460"
     CDS             complement(27982..28947)
                     /locus_tag="VGQ51_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9037037.1"
                     /translation="MDNTQHLITPEGTRDSRTWEFDGIRMGHALSRFNGLTSFSAHSP
                     HSDVIRLHIGLKGNYSWHYKQLDRDFHLIGGHHNLLYSKDFDMTVANRTPELETFGIQ
                     FPRETFLRMTDNASDILRRFADRIVAGESAMMSEEWGAVSPAIQQVVRQILDNPYKGD
                     LQQMFLLSKSIELLVLCAESCSAALSAKRGDDFIRTPADREKLAAVRDLINQRLDSPP
                     NLSEIARTVGLNEYKLKRGFKEMFRMTVFGYLTEQRLLLARQHLLDTRKTAAEISYEL
                     GYTTPQHFNNVFKRRFGETPDSLRTDSLRADSAGPPGEPKRRKNP"
     gene            complement(29023..29553)
                     /locus_tag="VGQ51_10465"
     CDS             complement(29023..29553)
                     /locus_tag="VGQ51_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037038.1"
                     /translation="MQQPPTLIPSRRPPYKKGFWCLIPLIGAFVGLTILLIGIFRYKD
                     VKYTLIGLLGIAWTVIIYSSLYLYSQSPTGREGFKFFTQNNLNQTVAEIELYHIQRGR
                     YPDSLQQLKSIDSFPPIWDPMQRVGKSPKLNYGRVGDKYFLFSSGLDGIPHTKDDLYP
                     QIAIHDSSKIGLIRPE"
     gene            complement(29578..>30068)
                     /locus_tag="VGQ51_10470"
     CDS             complement(29578..>30068)
                     /locus_tag="VGQ51_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV9037039.1"
                     /translation="QDILDDTGLSRSSLYDTYGDKHSLFLQSLRRYRDRETAAAIRFL
                     DESEDATLAIRQIFQHAYEVLVNMQQQKGCLMINTLHELVPHDPEVAALVFENRQALE
                     DAYTRAVRRGQQQGGISNTHQPRALARYLLNSLWGLTVHLKLGIAKRTADDIVKITLS
                     VL"
BASE COUNT         6687 a         7734 c         8818 g         6829 t
ORIGIN      
        1 atcttgttgt tgctgttcat cttccagcgt tttggaacga aggtggtggg aggctcgttc
       61 ggaccgatca tgatggtctg gttcctgatg ctggggtatg cggggttgcg gcaaatcgtc
      121 cactatcccg ccatattcgc ctcgctcaac ccggtctatg gactgaagat gctgatcaac
      181 catccccgcg ggttctggct gctgggatcg gtattcctct gcacgacggg tgccgaggcc
      241 ctgtattccg atctgggaca cgtgggacga aaaaacatct atttcagctg gaccttcgtg
      301 aagatcacgc tcgttctgaa ttatctcggg cagggtgcct gggcgctgat gcgcaacaag
      361 cccgacctgg aggggttgaa tccttttttt gcgatagttc cgagccagtt cctgctggtg
      421 agcatcatag tatcgaccgc ggcatcgatc atcgccagcc aggccctgat cagcggatca
      481 tttaccctga tcagcgaagc gatcagtctc ggtttctggc caagggtaaa agtgaaattt
      541 ccgacggata tccgcgggca gatctatatt cccagcatca actggatcct gttcctcggg
      601 tgtgtgctcg tggccctata tttccggacg agcgaggcga tgaccgcggc ctatggtttt
      661 tcgatcaccg tagccatgtt gatgaccacc atgctggtca cttattttct gcgctatgtc
      721 aaaaaatatc cattttggct ggtgggagcc atcgttacga ttttcctgat ggtcgagagc
      781 tgttttttcg tcgccaatgc ggtcaagctg gtcaaacggc tcttcttcct tgtcttcgaa
      841 ttcgggctga taaccacgat gtatgtctgg tacaatgccc gcaagatcac gcttcgtttg
      901 ctgaccttta cggatgtcgc tcccatattg ccgcgactcg cgctgctgag cagcgatacg
      961 agcattccga aatattcgat ccaccttgtt tatctcacac gggcgtcccg gcacgagatc
     1021 gagaacagga atgtggactc catcttcgac aacccgcgca aacgtgcgga tttctattgg
     1081 ttcgtcaata tcgaaagaca ggacgacccc tatacccagg aatatgacgt ggatatcctg
     1141 ataccgcaga aggtgatccg ggtcaatttg cggttgggtt tccggatcca gccactggtc
     1201 ggagtgatgt tgaggaaagt ggcggaggaa atgaaggcgg cgggcgaaat gccgatggtc
     1261 gggaaaccca aggatttcaa gtttgttatc ctggaaagat tcctctccta tgacaatgag
     1321 ttctcggtcc ggtccggatt catcctcaat acgtatttca gcatcctaaa gctggcaaga
     1381 tcggacagtc gcgcttatgg tatcgatagc gatcaggcgg tgatcgagaa ggtaccgctc
     1441 gtcgtggcgc ctgtacagag tattaccttg agacgcgtga cgaaggtgaa ggagcgcggg
     1501 catggggacc aggaaagcgg gatgccgttc tgaaagacgg gggcgccaga cgcggggctt
     1561 ccattaacga cctgataacg cttagattac ctaatttggg accccttggc atcgaattgg
     1621 acggctaggg tgtgatcatg aaagcttata ccgtttttga caatgggggt aagaccgtcg
     1681 accggtttac cgtcatcaat atgaagacag cagatgtgtt tgcggtcagc gataatcccg
     1741 gaactcccga tggcatcggc aggcattgcg gcaattgcgc cgaacatcgc atcgtactct
     1801 atggttcggg gtggaggcaa cgcctgccgc acaaaaaggt catcgaggcc gaggccgaga
     1861 attatatcaa caatgcgaga ctcgatcccg actggctcgg ccgtgagatc gaatttgcgg
     1921 cactgccggc gggcgtacgg tcttgtatag tcgatctcga gaaggacgtg gatccggggt
     1981 attattcgcg cgcgaagatc gcttcgctcg gaggttttcc gaggcaatcg aaaagtatca
     2041 atagtaagta gaggcgcggc gtgcagtggt cggggcgtgc agtggccggc gtgcagtggc
     2101 cggttcgcag gtcgcagtgt gcgggtcgcg gcgtgcgggt cgcggtgcgc aggggtgcgc
     2161 aggtggcggc tcgccccaag gcaaaaagcc ttatcttccc cctataaata cagacttcat
     2221 gccacaggac gtgccgcacc tctccacgaa agcaacagcg caaaacacct atgacgccat
     2281 cgtagtggga tccggaatca gcgggggatg ggccgcgaag gagctttgcg agctggggct
     2341 gaaagtgttg atgctggaaa gaggaaagga ggttcatcat ccgaattacc cgacggcgac
     2401 gctggcgccg tgggatttcc cgcaccggga gtcgctggac agggcagatc gcgagcggtc
     2461 gccgatacag gcgcgtcatt tttcttatcg cgaagacaac aagcattttt atatcgatga
     2521 tcgtgagaat ccgtatgagg aggtgaaacg tttcgactgg ataaggggcg atgtcgtggg
     2581 cggccggtcg ttgctatggg gccggcaatg ctaccggatg agcgatctgg atttcgaggc
     2641 gaatgtgcgc cagggtgtcg gcgtcgactg gccgatacga tacagggata tagcaccctg
     2701 gtatgaccat gtcgaaaaat tcatcggggt aagcgggaat gccgacggtg taccgcatct
     2761 tcccgacggg cattatcagc cgcccatggc attcaactgc gtggaagaat attttaccac
     2821 ccgaatcgca gcgaaatacg cggatagaag ggtcatcatg ggacgtaccg ccaatctgac
     2881 ccaacccgtg cagggaagag ggcaatgcca attccgaaat ctttgtcatc gcggatgtcc
     2941 ttatggcgca tatttcagca cgaacgcgag tacgctgcct gcagcctttg caacggggag
     3001 gctcacccta aggtcgggat cgctggtcaa caaggtcctc tacgataacg acgcgcaacg
     3061 cgcgacgggg gtcgaggtca tcgacaggga gacaggtaag accgtagaat actatgcacg
     3121 gctcatcttt cttaacgcag cgaccctcgc cacaaccttt atcctgctca actcgcgctc
     3181 caaccgctgg cccgaaggtc tgggcaatag cagcggacag ataggccgta acctgatgga
     3241 ccatcacaaa tcgaacagta taaccggcat catgccgggt ttcgaagaca aatattatta
     3301 cggccgccgg cccacgggtt tgtatatccc gcgctggcag aatttctcgg gtgaacatcg
     3361 ggattatatc cgcggttacg gatatgaagg atcggcatat agagccggag cgcatcccga
     3421 tgtctccatt ggcgcagcat tgaaagaggc ggcgacggaa cccggcccct ggaccatctc
     3481 cttcaaagga tacggtgaat gtcttcctta tgccgacaat cgcgtcgctt tgaataccga
     3541 taagaaagac aaatgggggc gcaatactct gagcgtggat gccgaattca gggaaaatga
     3601 aaaggcgatg aacaaagata tgatcgtcgc aatgcaggag atgctggatg cggcaggctg
     3661 caaggatatc agcgctggtg cggggatctc gtttccgggt aatgccaatc acgaaatggg
     3721 tactgcgcgt atgggaaggg atcagaagac ctcggtactg aatgggttca accagatgca
     3781 cgaggtgcac aatgtgttta tcacggatgg ttcgttcatg acttcttcag ggccggctaa
     3841 tccttcgctg acctatatgg cgatgacggc gcgtgcggtg cactatgcgg tggcgcagtt
     3901 gaaatagcgg gtaagtggaa caggatttga actgattggg ccgggccggg tggacatccg
     3961 gctggtactg aatcgttcat ctacaaaaag ggaatgtcat gagaagaaag atcgtgggga
     4021 ttatcctgtc cattttgggt attgccggcc tcgtatcggc ctttatttat atgaatggac
     4081 cggatagctc cgaacatttg gctgctttgc tggcccgggg cgtcctcggg gcaatcgcct
     4141 tttttgcagg catctggctg gtggatcata atgccgggcg acgtgcagtg taaggtgcga
     4201 ggaacaataa gcgcggcact ataataatgc gggccgacgc gctttataag gtatcgggtt
     4261 ggggttccgg gtgtagaaca cgcaaacctg gtcgacgaga tggtcgaata catcattctt
     4321 aatatagggt cgcgacgcct tcatcaacat atgcgtatta ttccggatca gatcaacttc
     4381 gcctttatcg aagatccctg agcggaccag cgtgtcgcgg acgaataccg ccatccggcg
     4441 atgatcctta cgatagttag cgttgccatt ttgctgtgcc tcgtcacgaa gccgctcgac
     4501 agaccggaga agctcaccct gcagggtagc cgccggaccg gacgccggca cgagatcgct
     4561 ccatagcttg taggcaagtt ccgggtcgcg taagggcgag atagcgggtt gaacggtgct
     4621 gatggcacgg atggcaggcc ttccggcttc ccagctggcg accatctcct ttacatcggg
     4681 tgcagcgagc tccagccatt ttcgggcatt cattccatag cggttcacct ggtcgagatc
     4741 ggcgccgcga cggattatct ttccaaacac gcgcagctgt tccgcgcggt tttcgtcatt
     4801 ctttctgagc agccatccct gtacggccca atagatcaca ttggagccgt cttcatcccc
     4861 gatattgaca tccgggttga aatccaggaa caggtccagg gctttgacat atctttcctg
     4921 ttgcacggcg atgtgcagac aattgtatcc gcgatgagaa cagatattcg gattggcgcc
     4981 tttctccagc agatagcgca acatcgggag acggtcctcc tcggattcga atccatagga
     5041 caagagcgcc tccatgagta cgggcatcca tgaccggctg tcggtaaagg cattgacgtc
     5101 aatcgcatag tcttcaacga ggtgttgtac tccggtggtg tcgcctttgg cgatggagag
     5161 ggaaagtgcg ctccagacgg aggcaggaat tagttttggc atcgagatca tatgggggga
     5221 ggccgtttgg ttgagcggcg acgagataag atacgaaata ttagttaaaa aatcaatatt
     5281 taaatgaccc ttttattttc tcgaggaaaa tttccttttt tcggcggctg acgtctattt
     5341 cggcgccatt gctcatgatc actgtcccgc cttcgccccg cctgtactcc cgcgtatggt
     5401 gtaaattgat gagaaatgat ttgtggacac gcaaaaagcc gtcttcgcca agcagcgact
     5461 cgtaatccgc caatggacgc gagacacaga tctttttgcc ggcggagaga taaaaatcgg
     5521 tatatggtcc cgccgcctcg caataaacga tctccgacag acttattaca tgaaaaccgg
     5581 tcagcgtggt caggcacagt ttcttctcct ggctattcgc ctgtggtttc aggttgtgca
     5641 tcaatgtgtc gatactcttt tgcaggttcc gctcagggag gatggtttgt tccgcccttt
     5701 tcacggcaat gaccaattcg ttctcatcca caggcttcag caaatagtcg atcgcgctga
     5761 atttaaaggc acgtaatgag tattcgtcgt gcgcgctgac gaaaatgatc cggaatcgga
     5821 tatcgccgat ctcctccaac aaggcaaaac catccttccc cggcatggca atgtccagaa
     5881 aaacgaggtc aggactgttc tcgctgatct taaccaacgc ctcgtccgcc gatcggcaga
     5941 cggctatcac attcagctgc gggcaattcc tatgcagcat ggtttgcaga ctgccgagaa
     6001 gacgcgcctc gtcatcgatc agaatggttt taatcatata tctccggggt cgtttaatat
     6061 aggtaatgtt aagacggcta aagtgccggt ctcgttggta tccagctcgc ttttgtcttt
     6121 gagctcgaga ttgcagtcca ggtgatactt ctcattcaaa agcgtcagcc ggtgccggat
     6181 gttgccgatg ccaaatgatt gatgtccgtg cctgggaagg agtttgctgt gactgatgcc
     6241 gatgccattg tcctctatct cgcaaatcaa ttgattatgc cccggcttgt agcggatgaa
     6301 cagtttgcgg tcgcccgatt tattcatcag gccatgccag atcgcgtttt ccgcgagtgg
     6361 ttgtaccatc atcggcggaa cacctatttc ctccgcacat attccctctt ctacttcgat
     6421 gtgataggta aacgaatgat cgaagcgcac gagttccatc cgaagataat tatgcagata
     6481 atccaggtct tccctgatcg tgacatatgg ccgtctggaa tattccagcg aaagccgtat
     6541 catcttcgaa aatttgttca ggtaatcatt ggcgttctcc tgttcatttt caaggatcat
     6601 ctgtttgata ctaccgaggc agttgaaaat aaagtgaggg ttcatttggg caagcagcga
     6661 actgagtatg gcatcgctgg tcttacgttc cagttgtgcg cttaatgcaa ctcgtttaat
     6721 cttgctataa taaagcagaa aacccgtggc cgcgcacaac agaataaaga ccccgatgcc
     6781 atacaagcgt tcttgcttca tatcctcacc aagccttgtt tccgtcagct ggttttgaaa
     6841 ccgcagggag tcggccttcc tgtcaaattc gtattggatc cctttgcgtt caagcgcctt
     6901 ttcccgctcc ttatcggtca ggctgtcccg gatggcgacg tattgtctgt attccgaaaa
     6961 ggctttccgg atattacctc gcttttcctg taatgaggac gatgtcttaa gaatgtctcg
     7021 cttcaccttc ttatcgcccg cctcttcagc cagcggcttt gcccggcgca atagcaattg
     7081 tacggtatcc agccgtgcgg tagggggtat aagatggcga agcaaggccg atgccggggc
     7141 atcaaggatc agtgccgctc ttttctgcag attatcggca atatcccgct tattgcctat
     7201 cgagtcgagt aacagggccg ccttgccata cgccgtcatt gctgcgtcct catcgccgag
     7261 ttccgaatcg atggatccca tataattaag taccatcgcg ataccatgtt tgttgccgag
     7321 cgtatcggcc aacgccagcg cctgtctgaa atcgaccatg gcggcggaat agcgcctgat
     7381 cttgagattc acggttccca ggctttggag attgaaataa agtccgaatt tttccccggt
     7441 ttgcctgcaa atggcgatcg acttttcata ataacctgag gcgcggtcgt aatcctctat
     7501 ttcttcatat agccagccca aattcgcata attgtgcgcc agacccgaga ggtcctttat
     7561 tgccccgttc attcgtgcag cgtccaacag ataatccata gctttgaggt attccgaacg
     7621 gtctgaatag atcattccta tatcctgaag gttctgagcg atcccgcggg tatccttata
     7681 tttttgatgg atggtcagcg ctttttggaa atagtcgagc gctttgggaa aatccgtctg
     7741 ttcgtaatag acgataccca tatcgccgta agtatcgccg atggattgtt cgtcattcgc
     7801 gtttgccgct atcgcgatcg accgatcgaa ataccagatc gcttttttcc aatcggccgt
     7861 ttgccagaat accatgccaa aatccgaata tgtgcggctc atccccttcg tgttgcctgt
     7921 tgcttcgaaa atatgcaacg cctgtccgag cgatgctata gcctctttgt attgaccgga
     7981 cctgtgataa agcttcccct ttgcctccaa tgcactggcc agttggtccc ggacgtgaag
     8041 ccgccgggcg agaacaatgg ccttgtccgc aaatcccagt cccgccgcta tattgcgata
     8101 ttgatagtcg tcggcaatct tcacccagcg gttaactgcc tcttcgtctt cggccgtctg
     8161 cgctttatcc aggcgcaaaa ggctgtcaag ttcatccatt cgctgcccgt aacagttttt
     8221 taccaacgcc ggacagacaa acaaaacacc aaaagcaagt atggaaagtc gcgacagcat
     8281 gtctgtacta agttacaaag tttacctctc cgaatttcac gaagaccgca gtgcatctgt
     8341 cccggcgcct atgagcaagc cggcgttccc gttccataaa gtaaccccgc cgttccgtaa
     8401 ttcatcgtgc gaagtcttcg cccggtgctg atctttggga ccggcatcga aacattttaa
     8461 ttaacgtaaa atagggaggc tgctttgaaa aagattatgt tcctgttgta tggggtcgtt
     8521 tgttatgcga tcttcttcgt gacgttcttg tacgctatcg ggtttgtaaa caatctgtat
     8581 gttccaaaat ccgtcgaaac gggttaccag ggagggacgt cctacgcctg gatggtcgat
     8641 atcctgctac tttcactttt tgcccttcag catagtatca tggcaaggaa agggttcaag
     8701 aaatggtgga ccaaaattat tcccgaaccc atcgaacgaa gcacgtatgt tttatttgcg
     8761 agtgcgtcgc tgatattgtt atttgcatat tggcgtccat tgccgggtgt gatctgggac
     8821 gtgcggaata atcttctctc gggtctgctg accggtctgt attttttcgg atggttcatc
     8881 gtcctgttcg gaacatttct tataaatcat ttcaatttgt tcgggctgca gcaggtatac
     8941 aataatctaa atgggagcga accggcgact tcgaggtttg tcaccccgat gttgtataaa
     9001 tttgtccgcc atccgatcat gctgggtttc atcatcgctt tttggtcgac tgtccgaatg
     9061 accagcggcc acctcctctt ttcggtggcg actaccgcct acattttaat cgccattcgg
     9121 ctggaagaac gcgatatggt tgcaatgtat ggagccgatt ataaacgcta ccaggaggaa
     9181 gtttcgcaga tcgtgccgat cccccgcagg aaatagaagg gatcgcaccc cgtcagccgg
     9241 cgggtgaggc atcgtccggg gcggcgaggc gggcgccgct aattcacgaa gatctcatcc
     9301 acgaatgccc accccggttt ccccttttcg atctgccagt cgggtagttt ggcaaccggg
     9361 gtcatcacga tccggagaca gcgtagtttc accggccgga acccgatatc atatcccggg
     9421 agaccggggg ccgtcgaagg tttggctgtg tcgggggtca gccggcccag cagccgcagg
     9481 tcatgggcgt tcgctccccc ccacacctcg atccccgccg gcggcatgat cgaggcgccg
     9541 gtattgatca tcgaggaaag tgtgacttcg tgtgcttcca cgggtttgtc gaaaagcagc
     9601 acgcattcca tcgggttgcg gcggaaaccg agccatttcc cgctggccca gttgaggtcg
     9661 cctttatcga ggtcgatgac ggtccggggg cccttccctt tataattgga atccagcggt
     9721 ttgagcatga tgaccgaatc ggggtggtgt ctatccgtat aaaatcttgt ttcgatggag
     9781 tcgcttggca gccatccttt tttgaaggcc cttactttga ggacggcgtt cgcgctgaag
     9841 agcgtgccgc cgctataaag cgtagaaccg gtgtcggggg aagtgccgtc gatggtatac
     9901 cggatagcca cacccggtac atagtgtttg agccgcagct gcatgggttg caaaagtatc
     9961 cgttcgtcat tctgcaggat gggcgggttc agccggatca gcatggtatc cgtgcggacg
    10021 cccatttgaa tatcgagacc gttgttgcct tttcccgagg aggcgaaggc ggaggcggcg
    10081 gaggaggggg aggcggaggc agatgcggag gcggcggagg cgaaggggga ggcggaggca
    10141 atgctccccg gggcaatggc ggtattccag agatagagat gacgcagtga tttcatcgtc
    10201 agcaaacagc ttatgtcctc cgcttttacc ggtgtccccg aaagactcag gcttttcagc
    10261 tgcgccagtc tgcacagctg tgcgataccg gcacccgtca ccgcggtaaa cgagagattc
    10321 agcgtgcgca gattggagaa ccggacgatc gcggggacat ccgcatcggt gaccggcatc
    10381 ttgtcgagat cgagcgaaac gatctggcgg gcaatcggtt ccaggtcgtt cagctggcca
    10441 ctccgaaaaa actgcgggct gtagaagtcg acattcagac cgggtgaatt caaggccacc
    10501 ggggatatga cgcgatagct ggtattcagt ttctgcaccg tattctcgtc ggcagacgga
    10561 aagtcatacc ggtcttcatc ctgcgcgttc ttaaacatcg cagccgccag ttgataaagg
    10621 gtgtcgttgg agggaagctg tgcgacgcgt atggtgcgcg tagggtcgtg tctcaaccat
    10681 tgatacagga tggtgatctc ctgatcggtg agctgcggct tgcctttggg aggcatatgt
    10741 ttctcgtcat cggggggaag atggacgcgg tgcatcatca gtccgaagtc ggcagcggag
    10801 ctatcccata ccgggccgtt cttcccgcct ttcagcaaca gctgcgtggt ctccatgatc
    10861 agctggcctt tcgccttact gctgttatga caacccatgc atttgttctg caggataggc
    10921 cggaccatat cgttgaatac cacggcatct tcgagcgaga ccttgcctgc ggaagaagca
    10981 gagctcaccg gcgccagcag gaaattatgt ccatgcgtga tatccgctcc tttgtcaccg
    11041 gtaaaggtca gcaacgccag gcatccgatg gcgacgccaa ctcccgcaaa gcgcaccgtc
    11101 tgcaaccgct ggtaccagtg gtaccaggcc agcgttaaaa acgaaacagc gacaccgctg
    11161 tatttatgcc agaaaagaac atcgggatca taccccggct ctttcgacaa aaacaagccc
    11221 attaaggctg tgagggcggc ggtgaatgcg gcgagtagca gcagcgtgtt gtcggcacgt
    11281 ctcaaaacca aacgctgaaa gacaaacaac accaccagcg cgatcgggaa atgcaggatc
    11341 agcggatgca tccgacccgc tacctgcagc caggtcggga ccaccaccct gctctcaaac
    11401 agtagcagga agaggagcag gcagttaatg gcaagggtta tattgaaaag caggttcttc
    11461 cagcgcatca ggagacgggt tccagtttat agggatacac atgccccgag ttccattggg
    11521 cgatataaag attttgctca tcatcgatac aaacgtcgtg cggatactgg aagaccttga
    11581 cggtctgccg catctcgccg agatgtccgc cgttatagtc gggctcgctg ccgccgaggt
    11641 ttgacacgac tttgaagtct ttgtcgagga tggttacgaa tcccgactcc tttcccacac
    11701 cagagctgct ttgcagcacg gccgcatata aataatctcc attgatcacc ggacggcata
    11761 cccaggcgcc gggaagggcg atggtgccga ggaatttccc ttccatcgtg tatcgtttga
    11821 aggcgttttg ctggcgggag gtgacgatca ggaagggtcc gccgggatga cggctgtcga
    11881 tggtgatccc gtgggcattc aaaaggtgct cctcgccgtc gccacgtccc ccgaaatgcc
    11941 ggatatagtt gcctttcgaa tcatactgga tgacgaagtc cttgccgtag ccatccgcca
    12001 catagatgtc tccgttaggc gcgatggtcg tctcggtagg gatgtattct tcggattttg
    12061 tatattggcc ggtctctttc gggtaatcca gcgtcatcag cacgcggccg tcgagggtcg
    12121 tcttgatcac ctggtgacgg ttgttgtcgc aaatgaaaag cacctcggta ccgttctcgt
    12181 cgaatagcgt caggccgtgc gcgccgggat attccgtacc ccaggcagtg agcagtttgc
    12241 ccttcctgtc ataaatgagc atattgttat gcgtgtcgtt ggtcaggagc aggatgcgcc
    12301 cgctgcggtc ctgtaccatt tcatgacagt ccttaacggg atagcgtcca aaatcgacct
    12361 ggctccagtg attgttcagg cgataacgtc gatggttttg gccgaggatt gtgtccgctg
    12421 tcagtattgg cggcacaaat agtatgcctg ctgcggcggt tgtttgttgt atgaattgac
    12481 ggcgttgcat attcatttgg tttgttagtc aggcgataat accatgcacg accttgccgg
    12541 cgacatcggt aaggcgataa cgtctcccct ggtgtttgaa ggtcagcttt tcatgatcca
    12601 gtcccatcag ataaagggct gtggcatgga aatcgtgtac atgaacggga tcttttatga
    12661 tattgtaccc gaaatcgtcg gtctccccaa atacgccggg tttaactcca ccaccggcca
    12721 tccagatcgt aaaacatctc ggatgatgat ccctgccata gttgtctttg gtgagcgtgc
    12781 cctggcagta gttggtgcgt ccgaactcgc ctccccagat gaccagggtc tcatccagca
    12841 gccctcgttg tttcagatcg gtgatgagtg cggcggaagc ctggtcgacg tcccggcatt
    12901 gtcccttgat ctccatagga agattcgcat gcccgtccca accctggtga tagagttgta
    12961 cgaatcgaac tccgctctcc gataattttc tcgcaagcag acagttcgcg gcaaaggttc
    13021 cgggcacgag acaatcggga ccatagagct tcagggtatc ttcgggctcc ttggatgtat
    13081 ccgttatttc cggtacgacc atctgcatgc gataggccat ctcgtattgc cggatcttgg
    13141 tgctgatctc gggatcgccg aattccttat aggattcttc gttgagcgtg gcaagtctgt
    13201 cgagcatgtc gcggcggtcg gccttggtca ttccgtccgg gtcgccgaga tagaggacgg
    13261 ggttttcgcc gttgctgaat tgaacgccct ggtgtatcga gtcgagaaaa ccgtttgacc
    13321 acagtttgga atagacgccc tgaccgttgc ctttgccttt ggaaagcagg acgcaaaaag
    13381 ccggaaggtt cttgttctcg ctgcccaggc catagctgag ccaggagccc atgctggggc
    13441 gattgccgac ctgtgcaccc gtttgcatga aggtgagagc ggggtcatgg ttgatggctt
    13501 cggtatacat gcttcggatg aaacagagat cgtcgaccac tcttgccgtg tgcggaagca
    13561 gctcgcttag ccataccctc gcctgtccgt attggttgaa cccgaaagcc gtgcctgcga
    13621 gcgggaattt tgtctgtgtg gaggtcatcg tcgtaaggcg ttgaccgagg cggatggacg
    13681 gaggaaggtc ctgcccctgc atttgttgca gcaggggttt atgatcgaaa aggtccagtt
    13741 gggacggcgc gccgttctga aagagataga tgatgcgttt ggctcgtgga gggaaatgag
    13801 gaagacccac ctgcggcatc tcgtctgcgc tatgtccgaa aagatcgggg atcagcagtg
    13861 agcccagcgc gatgctgccg acaccaaggc cggccctgta taaaaaccgg cgtcggttgt
    13921 atgttagcgc atgttcgagg atgtgtttat ccatgcttga cattttagga cctggtgatg
    13981 gtctcttcca ggttgtacag caattcgatg actttcataa gggcggcctg ctcgttcggg
    14041 tccagctgtt tgttttcggg atattcgccg atagcaagtg ttttcccggc gtccagcttt
    14101 ttctgcctga attcctgtaa ttgctcgtga taatagtccg tcaataccga gagttcttta
    14161 ggactcggat ggcgacagac tacggtccgg aaggcggcgg ttaaggggtc ggagcccggg
    14221 ttcgtcgcgg aggacgggtt cgtcgtgggg gacggattcg tcgtggggga cgaggtcgtc
    14281 gtggcggacg ggttcgacgt ggcggacggg gttgtcgtgg cggacgggtt cgtcgaagac
    14341 cggcgggtga gcagattttg cgccaatacg cgtgaggcct ccagtacggt cggatcattc
    14401 atcatcacca gcgcctgcaa tggcgtattg gtctgaagac gccggacctc gcactgatcg
    14461 cggttgctgg cgtcgaagat acccatcgat gggggcggca cggtgagctt aataaaggtg
    14521 tacataccgc gacggtatag cgagtcgtga tgatcctggc gataggcggc aagcgctccg
    14581 cgtccggacg tggccgcttc ccagagacct tccggctgat agggcttaac actgggtccc
    14641 ccgatggtct ttaccagcag cccgctgctg gagagcacga gatcgcggac gaattccgcg
    14701 ggtagccggt tgcgcggacc gcgcgataca aacgcattgt cgggatcctt ttctaatttc
    14761 tccggcgtga cgcttgtcga ctggcggtat gcggcggaca tcacgaaagt ctttaccagc
    14821 cttttgatat cccagccgtt gtccataaag tcgacggcca gccagtcgag caatgccggg
    14881 tctgtcggaa gatcgccctg catcccgaaa tcgccggagg acttgacgat accccttccg
    14941 aagaattcct gccatagctg gttgacaaag acccttgctg tcagcggatt ttgtcggctg
    15001 acggtccact ccgcgagacc cagccggttg cgtgggtatt tcgtcgtatc gaatggcatt
    15061 accgcgggca gggcggatgg cgacacttcc acgctcgggg cgtcataacg accgcggctg
    15121 agaacatagg tcttccgaag cgaatcgcgt tcacccatga ccgataccat cagggccgcc
    15181 gtatccgttt tatgaaggaa gggcatcagt cgtgcatcgc tgtccgtgat caggaggatg
    15241 ggtgttttgg caggtttcga tttggtcacg tccccttcgt atcccacttc tttggtatta
    15301 ttgaaaaaag ccagcagcga ataataatct ttttgcgaga aaggatcgta tttatggtcg
    15361 tggcattggg cgcattcgat cgacagaccc agtatgccct tgccgtatgt cttggttttg
    15421 tcgatgaggt attcgacccg gtattcttcc tgtatcaccc cgccctcctc ggtatatttg
    15481 tgattgcgga aaaaggcggt ggccagtatc tgctgtttcg tcgcgtcagg caacaggtcg
    15541 cccgctatct gccatgtaat aaaacggtcg taagacatat tgctattgaa ggcgctgatg
    15601 acccaatccc gccatggcca ttgggtccgg atattgtcgt cctgatagcc atagctgtcg
    15661 gcataacgcg cgacgtccag ccagtgtacc gccatcttct caccgtattg aggggtggcg
    15721 agatagtgat ccaccatttt ttcatatgca ttgggcgccc cgtccgccag gaatgcgtcc
    15781 tgttcggcga ccgtaggcgg aagacccgtg atatcgagag acagccgctt gagcagtcgt
    15841 tccttatccg cctcgggatt catcgcgaga cccatcttat cgagacggtc gatgacgaaa
    15901 tagtcgatgg cgttgcgggt cctggtcgtg tctttgatcg tgggcaaggg agcctttacc
    15961 ggcggtgtga aggcccagtg gcgttcgtat ttggcgccct ggcggatcca tttggtgaat
    16021 agcttttgct cgtggggcgt cagtacgccg aggtgtgcgt cgggcggcgg catccggaag
    16081 tcggggtcct gcgaagagac gcgtttcagg agcatcgagg cgccggggtt acccggaacg
    16141 atggcaaagg cccccttcgt cacatggagc ggagcatagg cactgtcggc tatatccagc
    16201 cgcaaatggg cttcgcggtg gttggcgtca gggccgtggc atttgaaaca tttgtcggag
    16261 aggatgggcc ggatatcgta gttgtaggaa accacatccg gaattttttc ggattccggg
    16321 ccgccgcgat ggtctaaaca ggaaacagcg agacagatag agccggcaat agatgtaata
    16381 atggcaagac ttcggaacct catacgcctg accaatataa tgaaaagtag cgagattcca
    16441 cgccgcaatc ggttgcgcca tctttgaggc caagaccgca agaatcgggt tttcggggtg
    16501 gcggcggcgt attaattttg taatagaaaa aagaaaatca tggaaacaac ccaggcgacc
    16561 atcaattata accggatagc ggaggccatc gccttctaca aggcgaattt caaggatcaa
    16621 cctggcctgg aaaaggtggc ggagcatgtc catatgagcc cgtttcattt tcaacggatg
    16681 ttcagggagt gggcgggagt aacaccgaag caattcctgc agtacctgac cctggagcat
    16741 gccaagaacg tcctgaaaca acctgccggc gcgacattgt ccgatgcggc atatgaggcg
    16801 ggtttgtcgg gcacgggcag actgcacgat ctcttcatcc ggatcgaggg gatgacgccg
    16861 ggtgaatata agaatggcgg ggaagcgttg cagatccggt atagttttgc cgaaagtccg
    16921 ttcggcccgt tgatggtggc gtctacagac aagggggttt gttatatggc gttcgtggat
    16981 aacgggaatg aggccgcgtt gcgcgacctg aaggaacact ttcccaatgc gcagtatgaa
    17041 gccggcgcgg acccccagca gcagcgcgcg ctgtcgatct ttacgggtga ctggacgagc
    17101 ccggaagcgt taaggctgca tctgaagggc acccctttcc aggtgaaggt ctgggagaca
    17161 cttctgaagg tgccggctgg tgggctcacc acctatgcgg aagtggcggc gaggctccaa
    17221 aacccgaaag cggtgcgggc cgtagggtct gctgtcggac agaatccggt ggctttcctg
    17281 atcccctgtc atcgcgtgat ccgcagcgac gggcaggtgg gggaatatca ttggggcgat
    17341 acgcgcaaga atgccatcat tggctgggag gcggcaaagg cgcatacggc gagtgagagg
    17401 gcgtagtcga cctactcggt ccgcaacgag tccaccgggt tggccagcgc cgcgcggatg
    17461 gactgaaagc tgatagtagc taccgctatc ccgaccgccg cggccccggc tccggcaaag
    17521 atccaccacg aaagaccggt ccggtaagcg aacccttcaa gccagcggtt catcaagaac
    17581 catgcaagag gtgctgcaat gagaaaggca atgccgatca gacggataaa atcgagggat
    17641 aacagtttga cgatcgtggc agcggaagcg ccgagtatct ttcggacacc gatctctttg
    17701 gtacgctgca gcgtgttgta tgccgcgagt cccaataacc ccagacagga gataaagatg
    17761 gccagcgccg caaaattgac gaataggcgg ccgaagcgcg aatccgcgcg gtattgctcg
    17821 tcgaagatct catcgaggaa tcggtattcg aacggacggt cgggcatcgc ctgcgcccat
    17881 tcggctgcaa tggtcttcag cgtcgccgga agaccggccc ctgccagttt caccgaaatc
    17941 gaataatatc cgaagggttc gatacgcatg accaggggcg cgatacgatg cttcatcgag
    18001 agattgtgaa aatccctgag gacaccgatg atctttcccc tccttcccca ctgggcaaag
    18061 tcccgtccga gcgcttcacc gggtttcgcg taacccagca tccgcgcggc actctcgttg
    18121 atgatcatgg cctgcgtgct atcggatgga aaactacgcg agaaatttcg gcccgcaacc
    18181 agttccaggc catattgagg aacgaaatca taatccacgg tatagagatc gagattggcc
    18241 ttttgcatct ctccctttcg atcctgcaat tcggtgtacg cgttgaacaa accttcaccg
    18301 ggaacggagg aagaataggt gctgccgagg acagcgggaa tctccgaaag cgattgtttg
    18361 aagacttccg cccgggcgtc gctcattgtg ggtataatga gcgtctgttc ttttttaaag
    18421 cccagctggc tgctgcgcat aaagctgagc tgagcgtaga cgacagccgt gccgataagc
    18481 aggaggatgg aaacggtgaa ctggctcacg acgaggacct ttctcagcag cgatcccctg
    18541 gctccggtgg aaaatcgacc tttcaggacg gtgacaggcc ggaaggaaga aaggacgaga
    18601 gccgggtaga aaccggctgc aaggcccacc agaatggcca gccccagaag gaggaggatg
    18661 tccgtgggtt gtgaaaagat gccggggcta atggtctttc ctgccagccg atcgaaaaga
    18721 ggcgtcagca cgccgcacaa gatcacggtc aataagaaag ccagcaggca aaggatcacc
    18781 gactcgccga tgaattgaag cgatagctgc gatcgcagcg ccccgatcac cttccggacg
    18841 ccgacctctt tggcacgatc gacactgcgc gccgtggtca ggttgataaa attgatgcag
    18901 gccagcgcga gtataaagag tccaatgatc gaaaagatgc ggacattttc gatactgccg
    18961 tgttgggccg cctcgcgttt ggaatacaaa tagaccctcg tcagcggttc cagcaggaag
    19021 gaaacgcgca actgtaacgg gcgaagctgg cttccggcga gtctgtcaag ccatgcgggg
    19081 agtttagcct ccagagcggt ggcggcggta tgcggcctca ataaaatgta agtgaaatag
    19141 ccaaatccgg cagtccagtc ggggctgtcg gggttatcgt ctccatggat gtgccggttg
    19201 gaagccatgg agagaaatat cccggtcctg atctgcgaat tggcggggat gtcctttatc
    19261 acccccgtga cggtcgcagt gagcgcgctt gcggtcaatt ggagatgccg gccgaggaca
    19321 tcggtagtac cgaaatattt tcttgccatc gactcggaga ggacgacgct catgggttga
    19381 gtcagggcgg tatttttgtc accggctatc aactgaaaat cgaatatatg gaacaagccg
    19441 gagtcggtta gcactacccc atgttcctgg aaccgccggt cgccggccgt cacgaggagt
    19501 tcctgcggac tcatgcgaac ggcttcttct atttcgggaa agtctttttt tagacggctc
    19561 gcgacgggcg ccgggcaacg accctgatag ctgtcggttc cggtcggcgt cgatatatcc
    19621 gggacgacgc ggtagatccg gtcggcattt gtgtgccacc ggtcgtaact ggtctcgaat
    19681 cggacatact ggaaaatgag aaagaaggcc gtcataccga tggccagacc gaggatattg
    19741 atcaccgaaa agacgcgatg ccgcaggatg gtgcggatgg cgatggtaaa atagcttttg
    19801 aacatagttg aaattattcg gtgcgtaagg cttttatagg attagcaacg gccgcacgga
    19861 cagattggta tccgacagtg gcgactgcga tcagcaccgc cagcactccg gaaaaagcga
    19921 agacatccca tcccatctct atccggtagc tgtaattgag caaccatttg tccatcacca
    19981 gccaggcgac gggcgcagag atcaggaaag ctacgagtac gagtgcaacg aagtccctga
    20041 cgagcagtgt ggttatgccg gcaacgcttg cgccaagcac ctttcgcacg ccgatctcac
    20101 ggatacgatt ctcggccata taggcggcga gagcgaatag tcctaaacag gagatgacga
    20161 tcgtcaacgc ggcgaacagc gccgacaacc gggtgatccg ttgttcggct ttgaattttt
    20221 ccgcatacgc ttcgtcgacg aaatgataat cgaagggata gtcggggctg tatcggtgat
    20281 agaccttttc tgcgagcgca agattggcgg ctgtagtatg cgcgggattc agcctgaaat
    20341 tgatcacctg gaaataaata cccccgggcc cggcaatcat catcggggcg atctttttcg
    20401 taaagggcga ttcgaggata aagtccttta tgacgccgac gacatgataa ggcgaggcgg
    20461 acccctgtct gatctcctga ccgagcgggt tcttcaaccg catggccttg accgcggatt
    20521 cattgagaag caatgcgctg ctgtcgccgg ggtatttgtc gatatcgata tcgcggccct
    20581 gcagtagggt gatgcccatc gtctgcacga agttggattt ggcgccgaag gtcagaaagt
    20641 ccacttcccc atcggccttc gtgctacctc gccattggaa tccccatgtg tcgctccagt
    20701 gccgggtgac tggtcccggg gtattggtga cggaaatagc ggcgccgctg gcgaggagtt
    20761 cgcggcaaat ggcatcctgg tgaccgaagg ctgcaccctg tgtataaaaa tagaccagct
    20821 gatcgcggtt gtagccggcg tcgcgatcga tgccgtagcg aagttgtttg aaaacgatgg
    20881 tcgtgcagat gacgagggcg atggcgaagg agaactgaag cacgacgagt atttttcggg
    20941 agttgacaag ggcaatcact ttccggaatg aatttcggct gagctgcgtt gacacgctca
    21001 gcctcattcg gaacccgggg gttcccttca gcacggtcac cggccggaag gaagaaaggt
    21061 aaaacgcggg gtagctgccg gccagcagac cggtgaagat aacaaacgcg acgccgaata
    21121 tccagaagcc ggggctggag tcatcgatgc tcagctgttc ccctaccagc ctgttgaaag
    21181 ccgggagact gatatgtgtc agaaaaatgg cggcgataaa ggcaagcgac gagatcagcg
    21241 tgctctcgcc gatgaattga gcgatcagcg accggcgcat ggcccccacc acctttcgga
    21301 taccgacctc acgggcccgg cgttcgctgc gcgccgtgct gaggttcata aagttgatac
    21361 aagcgatgag caggatgaag accgcgatag cggagaacag tctcaccgta acgatcttat
    21421 cgccgaccag ccgcccgtcc tgcgccttcg aatacagatg cagacggctt atcggttggg
    21481 tgaagacctc ggcctgctct ttcgaatggc tgcgcgtgac atccgcgacc tgtttgtcga
    21541 aagaagcctg cgtgacgccg ggtttcagca ggacaaaggt ggaaatggag ttgttccccc
    21601 agttctgctc gtcattccat ccgagatggg tcatatatgc ccacggtacc aggtaatcga
    21661 aggaaaagga cgttgttgca ggaaggtctt cgagtatccc ggaaaccgta aaattggcat
    21721 tgctgtcgag acggacggtc ttacccatcg ggtcccggtt gccaaaaagc cgaacggcca
    21781 tctgccgtgt gagtacgata ttgtacatgt cgccaagcgc gtgggcaggg tcgcccgcca
    21841 gcatcggaag gccgaacatc gtcagaaaac cgctgtcggc gaagccggcg atgcaattga
    21901 ggtggatgtc accaacggag gtaagaaagc ggtctcccgc atagcgggcg gcatcttcga
    21961 caccgggata gtcttttttc agaacaggac cgatagattt aggggtctgg tcccagacct
    22021 ggaccgcacc gttgaaggta ccccggttgt acaacatata caacctgtct gtttttggat
    22081 agatgcggtc gatgctcagc tcgttcttta tccagagacc gatcaggagt gcggaggcca
    22141 tgcctatcgc gagaccggtg atattgataa aggagaaccc tttatgccgg ataaggttac
    22201 gccacgtgag tagaaaaaag ttctttatca ttgataagta ttattcggac cgcaatgccc
    22261 tgacagggtt gccgagggct gcgcggatcg actgaaagcc gaccgtcaat agcgcaatcg
    22321 ctatggcaag gataccggcg agcaaaaaca cggtcccggt gatatgtata cgatacgtaa
    22381 acccgtgcaa ccagtcgtcc atcgcgatcc agctcacggg gatcgccaat accagggcaa
    22441 cgagcacgag cttgacgaag tctccggaga tcaacatcgt aatgcccgta accgaggcgc
    22501 cgagcacctt gcggatgccg atctccctca tacgactctc cgccatataa gccgccagcc
    22561 cgaacagacc cagacaggaa ataaagatgg tcaatgccgc gaaaagcgcg ccgagtgttc
    22621 cttcctgctg ttcggccctg aacttggcgt tatagatatc atcggcgaaa ttgatctcga
    22681 aaggatactg cgggttatac cggcggaaga tggatccggc ggacgccagc acgtcggcaa
    22741 ggggccggtc ggggttaagc cggaaatgaa taacgggata ggctgtgctg agcccctgaa
    22801 tgatcatcgg aacgatgggc tcatacggat tggcgatgat gaaatctttg atgacgccga
    22861 tcacgtgcag gtgtactcct gcatcgtcgg tgatgagggc gcccacggga tgatcgagac
    22921 gcatggtgcg gacggcagtt tcattgagca ggacagcaaa gctatcggta ggatgggtgc
    22981 cgagatcgat gtcccgtcct tccacgagat gtgtgcccgt agtatgcacg aagtctgcgt
    23041 ccgcctggaa ctgtgtaaaa tagaggccct tgtccgccgg cgtgctgccc gaccaggaat
    23101 agcccgtcgt ctcgccccaa acccgggtca tggggctgaa ggttttggtg acgccgacag
    23161 ctgttccgct ggacaagagg tcgtgccgga tcgcgtcata gtgcggaagg acatctccct
    23221 gggcaaaaac gaaggcgagc cggttccggt cgtatcctgc gtcgcggtcg cgcgcatatt
    23281 ttagttggtc ctccacgatc atggtggagg tgatcagaat gatggcaaaa gtgaattgca
    23341 gcaccacgag aaccttgcgt ggggtaatga gagcatttgc agtcctcatg gaaccgcttt
    23401 tcagcacctt taccggacgc gccgccgaca ggaaaaaggc tggatagcta cccgcgagaa
    23461 tgccggtgaa gaaaatgaag gcggcagaaa atatccagaa acctacgctc gtcagatcga
    23521 gcgtcagcgg aataccgatc acctcgtcga aggccggcag actgagcccg acgatcccca
    23581 gcgcaagcac aaaagcgatg gccgcgagca gaatactttc cccgatgaat tgggtaataa
    23641 gcgaggatcg cagcgcgccg gcgaccttcc ggataccgac ctcgcgtgcg cgttttacgc
    23701 tgcgggcagt gctgagattc atgaagttga tgcaggcgat caggaggatg aacacggcga
    23761 tgagaatgaa aagccggacg gtcgtgatgc ggcccgctac cagtctgccg ttgtccactt
    23821 gcgaataaag atgttcccgg gtgataggtt gtgtgaatat ctcgcgatcg aagccctgtc
    23881 ctgttttgac atggtcgcgt atgatgtgac ggatcttgtt gtcgaaggct gtagctgaag
    23941 tgccgggttt cagcagcaca taggcaccgg cattggtgat ctgccaggct accgccagcg
    24001 ggtcccagcc cagctcatcg acatgagccc aggggataag gtattgaaag gtaaattcgg
    24061 tattgggcgg aaggtctttg aggacccccg tcaccctgaa ctggcggtta ctatcgatga
    24121 gcaccgtctt acccatcggg tcttcattgc cgaaaaggct tttggcgaca tgccgggtca
    24181 gcacgatatc acggggacca ttaagcgcag tgcgtgggtt gccctcgagc agggggaagt
    24241 cgagtatgga caggaaaccc gagtccgcga aggcgccctc gagattgaaa cgggcatcgt
    24301 cctttatcaa caggaaatag acggttctga aacgggcggc gtcttcgacc tcggggtagg
    24361 tctgtttcag ctgtggcaca agaggcgtgg gggcgcgacc ccaggcggtg aggcggccgt
    24421 tggtcatttc gcggctatag atctggtaga cacggtcttt tttggagaaa aagcggtcga
    24481 cgctaagctc gttatgtatc cacagaccga taagcagggc gctggccata cctatggcga
    24541 gacctgcaat attgatggcg gagaacgcct tattgcggag cagattgcgg atcgcaatct
    24601 tgaaatagtt gcggagcatg ggatagtcgc cataaaagat atgccagaac ggcaattgac
    24661 tgatcgacaa tctgttggtg atgcgggggg acggcgggtg tccgcgggtg atacagccgg
    24721 tgtacgtagg cggccggggg gcggatggcg atcgggcgga tggcgatcgg agggttagtc
    24781 cgtgcggagc accatggccg gactggatag cgcggccttt atcgcctggt atccgacggt
    24841 gccggcggcg atacacagcg agagcacgcc ggtcagcggg aatatccacc agttcagatc
    24901 gatgtgatag ggaatcccat ccagccattg gcgcatgaac caccatgcta ccggcgaggc
    24961 gatgacgaag gcgatgcaaa cgagtataac gaaatctttt gaaagcagtg cggtcagtcg
    25021 catgatggag gcgcccagca cctttctgac cccgatctcg cggagcctcg actcggccat
    25081 ataggcggaa agaccgaaga ggcccagaca ggagataaag atggccatgc cggcgaagag
    25141 cgcggccagg gtgccgaaat gtttatcccc ctgcattttc gctgctacaa agtcgtcgac
    25201 atagaaatat tcgaagggat agtttggatt gtattttttt acgatggatg ccagggtccg
    25261 ttcgttgcgg gcacgatcgt tacgcggatt gaaccggaag gtcagcgcgc cgaaccagga
    25321 cgtcggtccc tggatgacaa tgggcagaac cctgttgaac agggtccccg ttatgaaatc
    25381 agcaaccaca cccaccacgt gataggtaaa gccgttattc accactgtct ggcctatggg
    25441 attggcgaaa cccatcgttt tggcggcttc gctggtgaga aggagcgcag tgctgtcgga
    25501 aggatatttt tcgacatcga tgtcacggcc ggcgagcacc ttcagatcca aagtagaggt
    25561 gagcccgcgg tcggtccggt attcgatgaa ccaggtctcc tgtttcggat ccttgccctt
    25621 ccactgatag ccatcacccg agctccatat ttcggtgaat ggcgcattgc tgcgggtaag
    25681 ttcggtgacg gcgccgctgc cgatcagttc gtcgcggatc gcagggtaat tcttgcggat
    25741 atcgcctttt atgtaaataa aagcgagccc tttcttgtta taacccatat cgcggtggcc
    25801 cgcatagtag aactcgcggt acatgacgat ggtgcagatg atgaaggcga tggcgaaggt
    25861 gaattgaaaa accacgagaa tccgtcttgg tgtaaccagc gcatgtatcg tcctgaaatg
    25921 tcctctgagg atattgacgg gccggaaggc cgagagatag aaggcgggat agctgccggc
    25981 cagcaacccg gtaaaaacga cgaaggcgat gccggccagc cagaagagcg gatcgtcgta
    26041 tgggatactc aaccccgtct cgatcagccg gttgaaccac ggtaatgcca ccacgacgat
    26101 gcccagcgcc aggatacctg ccagcgccgc gatgaggacc gattccccga gaaagcggct
    26161 gatcagcgat cccttccccg cccctaccac cttgcgtatg cccacctcgc gtgccctacg
    26221 gatgctgcgg gcggtgctga ggttcatgta attgatacat gcgatggcca ggataaaggc
    26281 agcaatgata gccagcatgc ggacgaaatt gatctctcct cccgtggcct tgccgtccac
    26341 gaaattggag tacagccgcc atttgcgcat gggatgaaga aagagcagcg agctcagttc
    26401 cggctggtgc gcgtgaagga ccgtgcgcag gcgactctcc gcgtcccctt ccgtaacacc
    26461 cggccggagg agtacatagg tcgcaatggt gctttttttc cagctggtct cctcccagtg
    26521 cacttctttg gtataggacc aggggatcag gtattcgaag gtaaatgcgg tattgggcgg
    26581 ctggtccttc atgacggcgc tgacgatgaa atcgcccgta ctgtcgaccc ggacgttgcg
    26641 acccagcgga tcatcgttgc cgaagagctt ttccgccagc cgtcgggtca gcacgatctt
    26701 acggggacca ttcagcgcgg tagcggtgtc gccttccagc agttggaaac tgaacagccg
    26761 gaagaaaccg gggtcggtaa ggtagccctg ggtcccgaca tgtttgtcgc cggcagacag
    26821 gatgaaggcg ccgacccaat ttagacgaac gacggtctcc acttcgtcac gatagcctgt
    26881 ctgcaatacg ggtcccaaca ccattggcgt gttgctattc acttccatac ggccgtcgaa
    26941 agagccccgg ctcatcacct gataaatccg gtcgcggttg gtatggaact ggtcaaaaga
    27001 aagctcgttc cggatccaaa ggagcagcac tatggcgctg gccagaccga tggcaagacc
    27061 ggaaatattg atcgcggtga atcccttgtt acgaagaagg ctgcgccagg cgatcttgaa
    27121 atagttgtcg atgagatcgc gggtctgtgc gaatgggccg gctttgacag cggcagccac
    27181 gggttcccga tgaatacgct ttagatcgcg gagcgccatc cgggtgagat ggcggttgaa
    27241 agccgaatcg gcgtccggca cttccggcga ctgccggcta cgccagagat ccgccaatac
    27301 ctgtagcgaa taggtcgatt ccggatgttg ttgttgaaat agttcgagtt ctgctatttc
    27361 ttccggtgtg gcctcgccgg ttagcttgcg agccatcaat atccatatgc ggtctttttc
    27421 cttcattcgg cgttgaccct ggtggtttcc gataaagatc ggaattatcg ggacaacctc
    27481 agccttcgac cagcgctttt atctgcacga gcatcggatc ccagccgccg cccctgaccg
    27541 agtgttcgta ccggacagcg ccttgcgcca cggtattgta atctccctgc gaaacggtca
    27601 gcagcgttcg gccgtgctct tcggagaact cgcaggtgac ggtcagatag ttcgcgggaa
    27661 tatcgggtat ctccgcgtta ttggggtcga tgacggtata gaccagcagc ctgttgggct
    27721 cgatggtgac gatatggcct tttaccgcca ccatctctac attattgaag acgcctttcc
    27781 agatgagcgg gctgcctggt ttccagtcgg aaagggcagc gcagccaaac atatattttt
    27841 tcgtttgttc ggggttggtc agggcatccc acactttggc ggcgggcgcg tgaatggaaa
    27901 tggagctttt tacgactagc ggctggttca tattctattt ttttgcaatg atccgatgaa
    27961 tgaaaccgcc gggattgcat tttagggatt ttttcttcgt ttgggctcgc cggggggccc
    28021 ggcggagtct gcccgaaggg agtccgtccg aagggagtcc ggagtctccc cgaatcgacg
    28081 tttgaacaca ttattgaaat gctgcggcgt cgtatagccc agctcatacg atatctccgc
    28141 cgccgtcttg cgtgtatcca acaggtgttg acgcgcgagg agaagccgct gttcggtcag
    28201 gtaaccgaaa acggtcatgc ggaacatttc cttaaatcca cgtttgagct tgtattcgtt
    28261 gagcccgacg gtccgtgcta tctcggaaag attgggcggg gagtcgaggc gctggttgat
    28321 gagatcgcga acggccgcga gcttctcccg atcggccggg gtcctgatga agtcatcgcc
    28381 gcgcttcgca gacaaggctg cgctacagga ttcggcgcat agcaccagca gctcgatgct
    28441 tttggaaagg aggaacatct gctgcaggtc gcccttatag gggttgtcca gaatctgtcg
    28501 caccacctgc tgtatggccg ggctcacagc gccccactcc tccgacatca ttgcgctttc
    28561 cccggcgacg atccggtccg cgaacctgcg aaggatatcg ctcgcgttgt cggtcatacg
    28621 aaggaaggtc tcgcggggaa attgaatgcc gaaagtctcg agctcggggg tccggttcgc
    28681 gacggtcata tcaaagtcct tcgaataaag caggttgtga tggcccccga tgagatggaa
    28741 atcgcggtcc agttgcttgt agtgccagct gtaattacct ttcaacccga tgtgcagccg
    28801 gatgacatcg ctatgcgggc tatgcgcgct gaagctcgtc agaccattga atcgtgatag
    28861 ggcatgtccc atgcggatgc cgtcgaattc ccaggtgcgt gaatcgcggg tgccttcggg
    28921 cgtgatcagg tgttgggtgt tatccacatc atgtaagata gtgtattttt aaggagggtt
    28981 tagaacgcca ctatcggaat acgacggtgc aggaaggatc acctattcag gccgaataag
    29041 cccgatcttc gaactgtcgt gaatagcaat ctggggatag agatcgtctt ttgtatgggg
    29101 aataccatcc aggccggaag aaaacagaaa atatttgtcg ccgaccctgc cgtaattgag
    29161 tttgggagat ttcccaaccc gttgcatcgg atcccatatg gggggaaaag aatcgatgga
    29221 cttcaattgc tgtaagctat ccggataccg cccgcgttga atgtggtaaa gctcgatttc
    29281 cgccaccgtt tgattgagat tgttttgggt gaagaactta aatccctccc tgccggtggg
    29341 gctttgcgaa tagagataga gtgaagaata aataatgacc gtccaggcga tgccaagcag
    29401 accaataagg gtgtatttga catccttgta cctgaaaatc ccgatcagca ggatggtgag
    29461 accgacaaag gcgccgatca gcggaatgag acaccaaaag ccctttttgt aaggcgggcg
    29521 gcggctggga ataagtgttg gcggctgttg cattattcgc ctaaagtaaa caatagtcta
    29581 aagtaccgac aaggtaattt ttacgatatc gtcggcggtg cgtttggcaa ttccgagctt
    29641 cagatgcacc gtcagccccc agaggctatt gagcagatag cgcgccagcg cgcggggctg
    29701 gtgggtgttg cttattcctc cctgttgctg accgcggcgt acggcacggg tatacgcatc
    29761 ttcgagggcc tggcggtttt cgaacacgag ggccgcgact tcggggtcat ggggcaccag
    29821 ctcgtgaagc gtattgatca tgagacagcc cttttgctgc tgcatattca ccaatacttc
    29881 ataggcatgc tgaaagatct gccggatcgc cagcgttgcg tcttccgact cgtcgagaaa
    29941 acggatggcg gcggccgtct cgcgatcgcg atagcggcgc aggctttgaa ggaacaggct
    30001 gtgcttgtcg ccgtaagtat cgtaaaggct gctacggctg agcccggtgt catccaatat
    30061 atcctgcg
//
LOCUS       DASYSV010000348         6466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1990828, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000348 DASYSV010000000
VERSION     DASYSV010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990828"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VGQ51_10475"
     CDS             complement(<1..476)
                     /locus_tag="VGQ51_10475"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9037040.1"
                     /translation="MRKKRLFVRVGVLCLLVLLGFPALSQKTVTGRVTDPAGKPLSGV
                     SVVPKGTGNGTTTDNQGQFRIDVNPSVKVLVFSSIGFTTREMPITGNVIDLSLATSNS
                     SLTDVVVIGYGAVKRSDVTGAVATISSKDFQTGAITTPDQLIAGKLAGVSVTPGSG"
     gene            complement(576..1337)
                     /locus_tag="VGQ51_10480"
     CDS             complement(576..1337)
                     /locus_tag="VGQ51_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV9037041.1"
                     /translation="MNQETIHNEAATDRHAVTQKDITTILKQLENDGIIGISVDCVIF
                     GFDDNELKVLLIKSDLEKYDNKWSLLGDLVNNHEDLDEAAYRILRERTGMDDVYLEQV
                     KAFGEVGRHPAARVVTIAYCSLINIEHHQLKILDNELHWHRVTTLTEMAFDHRKLLDA
                     CYEWLQKRIQEHPLGFSLLPKKFSLRELQNLYEAILDVKLDRRNFRKKFFSMDLLEDT
                     GELETDVPHRPGKLYKFNYDKYQKKERKKWFGIDF"
     gene            1402..3495
                     /gene="ligA"
                     /locus_tag="VGQ51_10485"
     CDS             1402..3495
                     /gene="ligA"
                     /locus_tag="VGQ51_10485"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV9037042.1"
                     /translation="MYSAGQTQNLQNLTTGMLADLKKGELPAKNIEDLRDVLRFHEYR
                     YAVLNDPLISDFEYDILYKALEKMEAEHPDLVTPDSPTQRVASGLTDEFPKVAHLVPM
                     LSLENSYNEDDLVDWDRKARELTGLQELEYCIEPKFDGASISLLYEDDRLSRGVTRGD
                     GVIGDEITTNIRQIRSVPLSAAFSRYGVEVIEIRGEILMNKNSFKKYNDGLTEQGLEP
                     LANPRNAAAGSLRIKYPKEVKRRNLEAFLYHVSYHTPLSKDPALLTHSGTLQMLWDLG
                     FRSPQKEKRIVRGIQAVIDYCREFERGRDELPYEIDGMVIKVNEIALQEKMGMTTHHP
                     RWAIAFKFAARQATSILRRVEFQVGRTGSITPVAKIDPVPLSGVTIGSISLFNEDVVR
                     EKDLRIGDQVLVERSGDVIPYIVKSLSDVRTGQETPIVFPVTCPVCGHKLVKPEGEAV
                     WRCVNIDCPAQVVERIIHFASKDAMDIRSLGDANIRKFYELDMLRTVPGVYRLPYDKI
                     RAQEGFGEKSVTNLRAAVEASKEQPLHRLIFGLGIRYVGETTAKTLAVSVNHLSEFSG
                     FSLEALQNLEDVGPKVASSVYAFFHDSENQRLLRELEELGVNLKSLKSSATPGGNLNG
                     QTFLFTGTLSRLKRTDAEEAVEAGGGKILSGVSSKLNFLVVGEDAGSKLEKAKKIPTI
                     QVLTEDEFIKLLEKA"
     gene            3591..4154
                     /locus_tag="VGQ51_10490"
     CDS             3591..4154
                     /locus_tag="VGQ51_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HEV9037043.1"
                     /translation="MIKKLTGEVWKPLQFPGWKQLRKKYALSSMGRVASYSEDVQADG
                     KLLNGSLTTGYKTLNLHRPGNNGTLYIHREIARLFLGKASPKNRYVIHVNHNKLDNAA
                     KNLKWATLEDMIDHQQKSPAKIAYKKVQANRTVGLKLTASQVKTIKKTLSSKNRQLTI
                     KKLAEKYKVSEMTMYRIKSGENWGRIK"
     gene            4203..4865
                     /locus_tag="VGQ51_10495"
     CDS             4203..4865
                     /locus_tag="VGQ51_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEV9037044.1"
                     /translation="MPQASTLKFLKDLKKNNNKPWFEKHKSLYLAAKEDVEHFAEEVI
                     RGLAKIDPDIAALQPKDCTFRIYRDVRFSKDKSPYKTNMGAYLTKGGKKSPTAGYYVH
                     FEPGRCFAAGGLWMPMAPDLNKVRQEIDYNFREWQDILGSRTFKRYFPEGMSREDILS
                     RPPKGYTEEDPAIEYLKLKSFIVIRKFTDADVLSASFLKKLLQTYSPVKDLVYFLNRA
                     ME"
     gene            complement(4915..5907)
                     /locus_tag="VGQ51_10500"
     CDS             complement(4915..5907)
                     /locus_tag="VGQ51_10500"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV9037045.1"
                     /translation="MKVAVVGATGLVGTKMLQVLAERNFPVTELIPVASEKSVGKQVE
                     FKGKKYKVVSAADAIAAKPAVALFSAGGSTSLEWAPKFAAAGITVIDNSSAWRMDPTK
                     KLIVPELNADVLTAEDKIIANPNCSTIQMVVALAPLHRKYKVKRIVVSTYQSVTGTGV
                     KAVTQLTNERKGISGEMAYKYPIDLNVIPQIDVFLDNGYTKEEMKMVNETRKIMRDDN
                     IRVTATTVRIPVMGGHSESVNVEFGDEFDLAEVRSLLCKAPGVVLVDDPATQQYPMPK
                     DAHEKDEVFVGRLRRDETQEKTLNMWVVSDNLRKGAATNAVQIAEYLADKGLLV"
     gene            complement(6090..>6466)
                     /locus_tag="VGQ51_10505"
     CDS             complement(6090..>6466)
                     /locus_tag="VGQ51_10505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEV9037046.1"
                     /translation="ALYNSFTVLDSLHLNGALTNGENIADIGGVAVAYDAFKLTPEGH
                     DSTKIDGFTPDQRFFLSFSRVWRIKEKDELVRTWINTNPHSPEKWRVNGPLMNNLGFY
                     TAFHVQPGQKMYRSDSTRIAIW"
BASE COUNT         1587 a         1652 c         1669 g         1558 t
ORIGIN      
        1 tggccgctgc cgggcgttac ggagacccct gccagcttac cagcgatcag ttgatccggc
       61 gtcgtgatag cgccggtttg aaaatcttta gaggagatgg tggcgacagc gccggtaacg
      121 tcggatctct ttactgctcc atagccgatg acgactacat cggtgaggct cgagttgctg
      181 gtcgccaatg aaagatcgat cacgttaccg gttatgggca tctcccgggt agtaaatccg
      241 atcgaagaaa aaaccaggac tttcacggag ggattcacat cgatccggaa ttgtccctgg
      301 ttgtcggtgg tcgtcccatt tccggtgcct ttaggtacca ccgatacacc gctcaggggc
      361 ttgccggcag gatcggtgac ccttccggta actgtcttct gggaaagggc agggaagccc
      421 aggagcacca gcaggcatag tactcctact cgcacaaaca atcgtttctt tctcatatgc
      481 aatacgtttt gtgtatttaa tacacagtat tatgtgtatt tagtacacaa tatagaggga
      541 ttttcgattt tttgtccgat tttcagggaa tttttttaaa agtcaattcc gaaccacttc
      601 ttcctttctt ttttctggta tttgtcgtag ttgaacttat agagcttgcc cggacgatgg
      661 gggacatcgg tctcgagttc gccggtatct tctaataagt ccatggaaaa gaacttctta
      721 cgaaaattgc ggcgatccag tttgacgtct aaaatggctt cgtataaatt ctgtagttca
      781 cggagcgaga acttcttcgg caggaggctg aagcccagtg gatgttcctg tatccttttc
      841 tggagccatt cataacaggc atcgaggagt ttgcggtggt caaaggccat ttcggtgagg
      901 gtggttaccc ggtgccagtg caattcgttg tccaggatct tcagctggtg gtgctcgata
      961 ttgatcagtg agcaatatgc gatggtgacg acgcgtgcgg ccggatggcg tcctacttct
     1021 ccgaacgcct tcacttgttc cagataaaca tcgtccatgc ctgtccgttc gcgcaggatg
     1081 cggtacgcgg cttcgtcaag gtcctcgtga ttgttgacaa gatcgccaag gagggaccac
     1141 ttattatcat atttctccag gtccgatttg atcagcagca ccttcaattc gttgtcatcg
     1201 aatccgaaga tgacgcagtc gaccgaaata ccgatgatac cgtcgttctc cagttgtttg
     1261 agtatcgtcg tgatgtcctt ctgcgtcacc gcgtgcctgt cggttgctgc ttcgttatga
     1321 atagtttcct ggttcattca actactaagg taaacaattc gaatactctt tacttaactt
     1381 tatgccatta aaagccaatt tatgtattcc gcagggcaaa cgcaaaatct tcaaaatctg
     1441 actaccggca tgctggccga tctgaaaaaa ggcgagctgc ctgccaaaaa catcgaagac
     1501 ctgcgggacg ttttgcggtt ccatgaatat cgttatgccg tcctcaacga ccccctgatc
     1561 tctgacttcg agtacgatat tttatacaag gcattggaga agatggaagc cgaacacccg
     1621 gaccttgtga cgcccgactc gcctacccaa cgggtggcca gtgggttgac ggatgagttt
     1681 ccgaaagtgg cgcacctggt gccgatgtta tcgctggaga attcttacaa tgaggatgat
     1741 ctggtggact gggacagaaa ggctcgcgag ctgacgggcc tgcaggagct ggaatactgc
     1801 atcgagccta agttcgacgg cgccagcata tctcttctct atgaggatga ccggctgtcc
     1861 aggggcgtta cccgcggcga tggcgtgatc ggagacgaga tcacgaccaa tatccggcag
     1921 ataaggtccg ttccgctgtc tgcggcattc tcccggtatg gcgtagaggt gatcgagatc
     1981 cgcggcgaga tactcatgaa caagaacagc tttaaaaaat ataacgatgg cctgaccgaa
     2041 cagggtctcg agcctcttgc aaatccacgt aatgccgcgg cagggtcttt gcgcataaag
     2101 tacccgaaag aagtgaaacg ccggaacctg gaagccttcc tctatcacgt aagctaccac
     2161 acgcccttga gcaaagatcc ggcgctgctg actcatagcg gtacgctaca gatgttatgg
     2221 gatctcggtt ttcgaagtcc gcaaaaagaa aaacgcatcg tcagaggtat tcaggcggtg
     2281 atcgattatt gtcgggaatt cgaacgcgga cgagacgaac ttccttacga gatcgatggc
     2341 atggtcatta aggtgaatga gatcgcatta caggaaaaaa tgggaatgac cacccatcat
     2401 ccccgctggg ccatcgcatt taaattcgcg gctcgtcagg ccaccagcat cctgcggaga
     2461 gtagagttcc aggtgggacg tacgggcagc atcaccccgg ttgcaaaaat cgatcccgtt
     2521 ccgctcagcg gcgtaacgat aggctccata tctcttttca acgaggacgt tgtccgggaa
     2581 aaggatcttc gcatcggcga ccaggtcctg gtcgaacgat cgggggatgt cataccatat
     2641 atcgtcaagt cgctgtccga tgtccgtacc ggtcaggaga ccccgatcgt tttccctgtt
     2701 acctgcccgg tatgcggtca caaactagtg aaacccgaag gagaggcagt ctggcgatgc
     2761 gtgaatatcg actgtccggc ccaggtggtc gaaaggatca tccacttcgc cagcaaagac
     2821 gcgatggata tccgtagcct gggagatgcc aatattcgaa aattctatga gttggatatg
     2881 ttgagaacgg tcccgggcgt ctatcggttg ccttatgaca agataagggc gcaggaaggc
     2941 tttggagaaa aaagcgtcac caatctgcgg gctgcggtcg aggcttccaa agaacagccg
     3001 ctgcatcgcc tgatcttcgg gctgggtatc cgttatgttg gggaaaccac ggccaagact
     3061 ttggcggtat cggtcaacca tctctcggaa ttttccgggt tctctctcga agcccttcaa
     3121 aatctggaag atgtgggacc aaaggtggct tccagcgtct atgcattttt tcacgacagc
     3181 gaaaaccagc ggctgctacg ggaactcgaa gagctggggg tcaacctgaa aagcctgaaa
     3241 tcctcggcta cgccgggagg caacctgaac gggcaaacgt tcttattcac tggcaccctc
     3301 agccgtctca agcgaacgga cgcggaagaa gccgtcgagg cgggtggcgg caagatactg
     3361 agcggtgtca gcagcaagct gaacttcctc gttgtcgggg aagatgcggg cagtaaactg
     3421 gagaaagcaa aaaaaatacc aacgatacaa gtactcaccg aggacgagtt catcaagctg
     3481 ctcgaaaagg cttgaacggt ccgcgaacat gcgtccgaca tataagatta ttttcttacg
     3541 tcactggtaa atcgaaattc tttttcgtaa ctttaagaaa attgacaggc atgatcaaga
     3601 aactcaccgg cgaagtgtgg aaacctttgc agtttcccgg ctggaaacaa ctccgaaaaa
     3661 aatacgcact gtcttccatg ggtcgcgttg ccagctactc cgaagatgtg caggccgacg
     3721 gcaaattgct gaacggcagc ctaactaccg gttacaaaac gttgaatctg caccggccgg
     3781 gcaataatgg tacgctttac atccatcgcg agatcgcccg cctcttcctt ggcaaggcgt
     3841 cacctaaaaa ccgatacgtg atccatgtga atcataacaa gctggacaat gccgccaaaa
     3901 atctgaagtg ggcgacgttg gaagatatga tcgatcacca gcaaaagagt ccggcgaaga
     3961 tcgcgtataa aaaggttcag gctaatcgca ctgtcggtct caagttgaca gcatcacaag
     4021 tgaagaccat caagaaaacg ctgtcgagca agaaccgtca gctcacgatc aagaagctcg
     4081 ccgaaaaata taaggtaagc gaaatgacga tgtaccgtat caaaagcggt gaaaactggg
     4141 gaaggataaa atagtctgcc cgaagacgat tgcacgaaaa tacagaccgg gaaaccaacg
     4201 ccatgccgca agcctccacg ctcaaattcc taaaagacct caagaaaaat aacaataagc
     4261 cctggtttga aaaacacaaa tctctctatc tcgccgcaaa agaggatgtc gaacattttg
     4321 cagaagaggt gatcagggga ctcgcaaaga tcgatcccga tatagctgcc ctccaaccga
     4381 aagattgcac cttccgcatc tatcgcgatg tccgtttctc aaaggacaaa tccccctata
     4441 aaacgaatat gggcgcctat cttaccaaag gaggcaagaa atcgcccaca gcggggtatt
     4501 atgtacattt cgaaccaggc aggtgcttcg ccgcaggcgg tctctggatg cccatggctc
     4561 ccgacctgaa caaggtccgt caggagatag attataattt cagggagtgg caggatatcc
     4621 tgggcagccg aacattcaag cggtatttcc cggaagggat gtcgcgtgaa gacatcctgt
     4681 cgagaccgcc caagggatat accgaagagg accccgccat cgaatacctg aaactcaaaa
     4741 gctttatcgt cattcggaaa ttcacggatg ccgatgtgct ctcagcgtct ttcctgaaga
     4801 agttgctgca gacttactca ccggtaaaag acctcgtcta tttcctcaat cgtgcaatgg
     4861 aataagcatc gatgggtatg cgcagtcacc ggatgcccgc ccggccgcgc gtcgtcatac
     4921 caacaaccct ttatcggcaa gatattccgc aatctgtacg gcgttggttg ccgcaccttt
     4981 gcgcaaatta tcgctcacaa cccacatgtt cagggtcttt tcctgtgttt catcccgtct
     5041 cagccggcct acgaatactt cgtccttttc atgagcgtct tttggcatcg ggtattgttg
     5101 cgtagcagga tcatccacca atacaacccc gggcgccttg cagagaagcg aacgaacttc
     5161 ggcaagatcg aattcgtccc cgaactccac attgaccgat tcactatgtc cgcccattac
     5221 ggggattcgt accgtcgtag cggttaccct gatattatcg tcgcgcatga tcttcctggt
     5281 ctcgttgacc atcttcatct cttccttcgt atatccattg tcgagaaaga cgtcgatctg
     5341 cgggatgaca ttgagatcga tgggatattt atatgccatc tcgccgctga tgccctttct
     5401 ttcgttggta agctgagtca ccgccttcac tccggtgccg gtcacgctct ggtaggtcga
     5461 caccacgatc ctttttactt tgtatttcct gtgcagcggg gccagggcca ctaccatctg
     5521 tatggttgag cagttggggt tggcgatgat cttgtcttcg gccgtgagga cgtcggcatt
     5581 cagctcgggc acgatcagtt ttttcgtcgg gtccattcgc caggcggagg agttatcgat
     5641 gaccgtgatt cccgcagccg caaacttcgg cgcccactcc agcgaggtgc tgccgccggc
     5701 ggagaacaag gctacggcgg gttttgcggc aattgcatcg gcggcgctga cgaccttgta
     5761 tttctttccc ttgaattcga cctgcttacc gacggatttt tcggatgcga cggggatcaa
     5821 ttcagtgacc gggaaattcc tttcagcgag gacctgtaac attttggtgc cgaccagtcc
     5881 ggtggcgccg actactgcaa ctttcatttt ttggttttat ttaaaattaa aaagcctccc
     5941 cgctctgcgg gaaggccttg atatttttac atacaaaaag atcagatcct gtcccgctgt
     6001 gggagtacct tcttgatctg cttgatatgt gtcatttttt gcattgcggg cgtaaaaata
     6061 gtcaggaaag cgggtccgtc caaatttttt taccagatcg cgatcctcgt gctgtcggat
     6121 cgatacatct tctgccccgg ctgcacatgg aatgccgtat agaatcccag gttattcatc
     6181 agcgggccgt tcactcgcca tttctccggt gaatgcggat tggtattgat ccacgtgcga
     6241 accaattcgt ctttttcctt gatcctccac acgcgtgaaa aagaaaggaa gaaccgctga
     6301 tccggcgtga agccgtctat ttttgtcgaa tcgtgacctt ccggcgtcag tttgaacgcg
     6361 tcataggcca ctgcaacgcc tccgatatcg gcaatgtttt ccccgttggt gagcgccccg
     6421 ttaagatgca gggagtccag caccgtgaag ctgttataca aggcga
//
LOCUS       DASYSV010000349         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_68498, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000349 DASYSV010000000
VERSION     DASYSV010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68498"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VGQ51_10510"
     CDS             <1..997
                     /locus_tag="VGQ51_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEV9037047.1"
                     /translation="VIVGCGEERGIGRDRREPGGGAPDTIREQFYQLYYWHSDVRLAD
                     VGNVRTGATLADTYAALKTVLREITAIGKTAVILGGSHDLTLAQYYTYSDKKHIIEAT
                     GVDALIDLDIHSLNRSDNFLMEMLTGEPNYISHYNHIGFQSYYVHPHMLETMDKLRFD
                     CFRLGSVKDNIEEMEPVIRNSHLFSFDISALAHSFAPANSLSPNGFNGEEACILMRHA
                     GLSHNVNTVGIYGYMPGKDRDQLTARQISHMLWYLIDGKSKGRREASLSDRDLFVEYR
                     TAFAEVETTFLQSKKTQRWWMQLPDSKYIACSYQDYLLASNNEIPERWLRAQERG"
     gene            1040..2416
                     /locus_tag="VGQ51_10515"
     CDS             1040..2416
                     /locus_tag="VGQ51_10515"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV9037048.1"
                     /translation="MRKRLLGCLLIGASCWQACTTTKTVGSAASKDPLLQFTQDSLLG
                     QPGLRPAQVGISLYDPATSTRLFDYQGDKYFMPASNTKLFSLYAGLKYLGDSLIGIRY
                     QLTDTALFIIPTGDPTLLHPAYADQPVIRFLQKAGRNIYLSDNNWKDKPFGRGWAWDD
                     YNDDYAPERSALPVYGNYIRWKFDSVSASFYSIPDIDWPVRFSPDSSRRGFFVQRDFH
                     GNAFEIDEGSGGAREQDVPFITNGIASAALLLKDTVGRPVKVTTWLSGGHHRAYGPAE
                     SIGNLPDMKVIHSRPVDSMYVPMMHNSDNFFAEQTLLMVSNEVLGQMNDGRIIDTLLK
                     TDLKGLPQRPIWVDGCGLSRNDNFTPQDFIWLLDTMRKQFGLERMERILPTGGKGTLS
                     SYYRQDAGYIFAKTGSLSGVVALSGYLITKKNRLLLFSILINNYTGSGVTVRRQMEKL
                     IHHIRDSY"
     gene            complement(2413..>2994)
                     /locus_tag="VGQ51_10520"
     CDS             complement(2413..>2994)
                     /locus_tag="VGQ51_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEV9037049.1"
                     /translation="KIMPIENAPLQQLLNTYIVTPTNRSFILVHQQAAHERILYERFQ
                     TASVGTRIPSQKSLFPVTLTLSAPDSILLTELLPDLQLLGYDIGPFGKDSFIIQGTPA
                     DVAGGNEKGVLENLLEQYKNFTSEIRFSKREKLIRTMARQQAIKPGATLTEKEMRTLV
                     DELFACAQHNITPGGDPTYIEFKKDYVERLFGR"
BASE COUNT          647 a          810 c          816 g          721 t
ORIGIN      
        1 cgtgatcgtg ggatgcggcg aagaacgcgg tatcggtcgc gaccgccgcg agccgggagg
       61 aggagcaccc gataccatcc gcgaacagtt ctaccaacta tattactggc attccgatgt
      121 acgtctcgcc gatgtcggta atgtccgtac cggcgcaacc cttgcggata cctatgccgc
      181 gttgaagacc gtgctccggg agatcactgc catcggaaaa actgcggtca tacttggcgg
      241 ctcgcatgac cttacactgg cacagtatta tacttactcg gacaagaaac acatcatcga
      301 agccaccggt gtggatgccc tgatcgatct ggatattcat tctctgaacc ggagtgacaa
      361 tttcctgatg gaaatgctga caggcgagcc caattatatt tctcattaca accatatcgg
      421 tttccaaagt tattatgtgc atccgcacat gctggaaacc atggataagc tgcggttcga
      481 ttgtttccgg ctgggtagtg tcaaagataa tatcgaagag atggagccgg tgatccgcaa
      541 ttcacatctc ttcagcttcg acatttcagc cctggcgcat tccttcgctc ccgccaattc
      601 cctttctccc aacggcttca acggtgaaga ggcctgtatc ctgatgcgtc atgcaggact
      661 tagccataac gtcaacaccg tggggatata tggctatatg cccggaaagg accgcgacca
      721 gctcaccgcc cggcagatca gccatatgct gtggtatctg atcgatggga agagcaaagg
      781 ccggcgggag gcctcgcttt ccgaccgcga tctctttgtc gaataccgta ccgcattcgc
      841 ggaagtggag acgacctttt tgcaaagtaa gaagacgcaa cgctggtgga tgcagctacc
      901 cgatagtaaa tatatcgcgt gctcctatca ggattacctg ctggccagca acaatgagat
      961 ccccgaaagg tggctgcggg cacaggagcg cggctaagtt tactaattca ctatattgcg
     1021 actaagaatt tctttaccga tgagaaaacg actacttgga tgtctcctga taggcgcttc
     1081 gtgctggcag gcctgcacga ctacaaagac tgttggttcg gccgcctcca aagacccttt
     1141 actgcaattt acacaggact cgctgctcgg tcagcccggt ctgcgcccgg cgcaggtggg
     1201 tatcagcctc tatgatcctg ctacgtctac ccgtcttttc gactaccagg gcgataaata
     1261 tttcatgccg gccagcaata ccaagctctt ctctttatat gccgggttga aatatctcgg
     1321 ggactcgctc atcggcatcc ggtatcagct gaccgatacg gcgctattca tcatccctac
     1381 cggcgatccg accctgctcc atcctgccta tgccgatcag cccgtgatcc ggtttctgca
     1441 gaaggctggg cgcaatattt atttgtccga caacaactgg aaggacaaac ctttcggtcg
     1501 cggctgggcc tgggatgatt acaatgacga ctatgcgcct gaacgtagcg ctctgccggt
     1561 ttacggtaat tatatccgct ggaagttcga ttccgtttct gcctcttttt attccatacc
     1621 cgatatcgac tggcccgtca ggttctctcc ggattcgtcg agacgcggat ttttcgtgca
     1681 gcgggatttt catgggaatg cgttcgagat cgatgaaggc agcgggggcg cccgcgagca
     1741 ggatgtgccc tttataacga atggcattgc ctccgccgcg ctgttgctga aagataccgt
     1801 cggccggccg gtgaaggtga ctacgtggct ttccggcggc catcatcggg catacgggcc
     1861 cgcggagtcc attggcaatt tgcccgacat gaaggtgatc cactcccgcc cggtcgactc
     1921 catgtacgtc cccatgatgc acaacagcga caatttcttt gccgagcaga ccctgctcat
     1981 ggtgagtaat gaggtgctgg gccagatgaa tgacgggcgt atcatcgaca ctttgcttaa
     2041 gaccgatctg aagggccttc cgcaacgccc catctgggtc gacggctgcg gtctcagccg
     2101 gaatgacaat tttacgccgc aggattttat ctggctgctt gatacgatgc ggaaacaatt
     2161 cggcctggaa cggatggagc ggatccttcc caccggaggg aaaggcacgc tgtcttccta
     2221 ttatcgccag gatgccggct atatctttgc gaagacaggg agtctttccg gagtagttgc
     2281 gctcagcggg tatcttatca cgaagaagaa ccggttactc ctgttctcca tcctgatcaa
     2341 taattatacg ggcagcggcg tgacggtccg ccgccagatg gaaaagctga tccatcatat
     2401 ccgggattca tattagcgcc cgaatagccg ctctacgtag tcttttttga attcgatata
     2461 ggttgggtcg ccgccgggcg tgatattatg ctgcgcgcac gcgaataact catcgacgag
     2521 ggtccgcatt tctttttcgg tgagtgtggc tccgggtttt atcgcctgct gacgggccat
     2581 ggtgcggatc agcttctcgc gtttggagaa ccggatctcg ctggtaaaat tcttgtactg
     2641 ctcgagcagg ttctccaaca cccctttctc attgcctccg gcaacatcgg cgggcgtacc
     2701 ctggatgatg aagctgtctt ttccgaaagg tccgatgtca tatcccagca gctgcagatc
     2761 gggcagcaat tcggtcagca ggatcgaatc gggagcggag agcgtcagtg tgacggggaa
     2821 aagactcttc tggctgggga tgcgcgtgcc caccgaggcc gtctgaaaac gctcgtacag
     2881 gatgcgctca tgcgccgcct gctgatgtac gagaatgaag ctgcggttcg ttggggtgac
     2941 gatatacgtg ttgagcaatt gctgtaatgg cgcgttttcg ataggcatga tctt
//
LOCUS       DASYSV010000350         9143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1093875, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000350 DASYSV010000000
VERSION     DASYSV010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9143
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093875"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            665..1438
                     /locus_tag="VGQ51_10525"
     CDS             665..1438
                     /locus_tag="VGQ51_10525"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV9037050.1"
                     /translation="MEKILVTGGTGSLGSEVVRQLISLGCNVGVFTSRRDPQVPDGVG
                     VFHGDLSTGEGLAEALAGVRTVIHCASNFSSFKDTDIGGTGKLLDTVDTSRPPHVVYI
                     SIVGIGKSVYPYYVAKRAVEEMLLGSGVPCTILRTTQFHDFVLNMIRTVLESAGEIAL
                     VPDGIRFQSVDIREVAGELIRIGGAKPAGLLPDFGGPEVLRFEDMFEEYVGMCLPGRE
                     WKIAPVAGMRYDNFRTGINIVPGQRSGKITWKEFLRSKF"
     gene            1597..3765
                     /locus_tag="VGQ51_10530"
     CDS             1597..3765
                     /locus_tag="VGQ51_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEV9037051.1"
                     /translation="MVSLADNAFSMKKVFFVLLIAAYFGVHAQAPARYPFQDRDLPVE
                     KRIDNLLSLLTIDEKITCLSTNPTIPRLGLKGTGHSEGLHGLALGVPGGWGRKTPVAT
                     TTFPQSYGMAETWDTALIRQAAAIEGYEARYTNQTLGKGGLVIRAPNADLGRDPRWGR
                     TEECYGEDAFFNGTLVQAYVRGLQGPDPRYWQAASLMKHFLANSNENSRDSSSSDFDE
                     RLWREYYSVPFRMGVEAGSRAYMASYNKVNGIPQAVNPSLRKVTVDEWGQDGIICTDG
                     GAYRMLLTAHHYFKTPEEAAAACIKAGINQFLDNYRAGVKGALEQHLLTEKDIDTALR
                     GVCRVMIRLGLWDDSTLVPYASIRGGEPPFMNARNKQFVRLLTQKSIVLLKNTDHFLP
                     LDKAKLHSIAVLGSRSEQVIGDWYSGSPGYAVSPLQGIIDKLGAGFSVNHTTNNDLAV
                     SLAKTSDVAIVCVGNHPTGGENKGWKQVSVPSEGREAVDREHIDLDSAQESLVERVMA
                     VNPHTIVVLISSFPYSINWIEEHVPAVLHMTQNSQELGNALADVLFGDVDPGGRLVQT
                     WPKSLDQLPPMMDYDIRHGRTYMYFKGEPLYPFGYGLSYTNFRYGNLRLSSPAMRDAV
                     DISVDVTNTGDREGDEVVQLYVSHEGSRVARPVKELRGFSRITLRPNETRAVVFRLTP
                     KDLAYWNVARHRFETEKDQIRVVLGGSSADERATARFKVL"
     gene            3804..4736
                     /locus_tag="VGQ51_10535"
     CDS             3804..4736
                     /locus_tag="VGQ51_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV9037052.1"
                     /translation="MYYLFYGFLYLLSLLPMRLLYLVSDAIYGIVYYVFGYRKKIVMN
                     NLRQAFPEKTEAECTRIAKKFYRNFIDSFIEVIKLVSASDAWVSKRFTADVAVLDELY
                     ATGKACQLHLGHTFNWEWGQLALTPLTKYKILVVYMPISSKMLEKLFYRLRIRSGNVF
                     LPAPKMREAMVQYLPTQYLLGLVADQCPGNMQTSWWIDFMGRPAPFASGPEKGARAGQ
                     LPVVFASIEKPRRGYYHATVQLACPDASQLPEGELTRIYVRYMEDVIRRRPEMWLWSH
                     RRWKHGWKEEYEPMRIDEGRVSNTYRWKESDAVK"
     gene            complement(4703..5515)
                     /locus_tag="VGQ51_10540"
     CDS             complement(4703..5515)
                     /locus_tag="VGQ51_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037053.1"
                     /translation="MPRLHLILAFVLTAQLTHAQDSTILHLNAGIIYNSGLNYYGRID
                     SLRSKGICPFIGLSLNDGLYLNSTFVFLENSQQSQYAATLLEAGYNFGGGNSSNHWAG
                     NLSVSRFLYEQNIDLVESVIKEMASASLTRLNNVANITLTGSARWSDRIDYSLQTGLD
                     HIIRILNVGTKKGVLVFDPTATISAGTQNFTTTYYEQKYFLFLPTQQQQITTSSEQFN
                     ILAYELSCPVVYGLGKMKLLLTPAYVLPRNIVAGSELASNLFYFTASLSFHL"
     gene            complement(5574..5981)
                     /locus_tag="VGQ51_10545"
     CDS             complement(5574..5981)
                     /locus_tag="VGQ51_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037054.1"
                     /translation="MKRAIIATIVIAFLGISNHSFAQIRLGAVANARLSSAASVNTPA
                     VSNALRASAAATTTTAATTAHTAAATTAHTAAKSTQTAAQAASKASQTSASTVKAARS
                     TGASTNMQSSTSASATKKSVGVSTSASTNVSVH"
     gene            complement(6072..6806)
                     /locus_tag="VGQ51_10550"
     CDS             complement(6072..6806)
                     /locus_tag="VGQ51_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV9037055.1"
                     /translation="MDSYDNEWIIRYVDGELSPEEAGQFEAAMRTDTALATEVERYRE
                     LKAVLHERLPGDPTLDALKQRTARLNEQFFQQTRIRKAPTLRFLSTVAAAACIIAVII
                     ISWPSETTRKLDQLGETSMIGITERGNQTDTLLQKASTFFNEKQFDKALPILNQAVIA
                     DSSSQLALFYRGVAAWHTGDLHVARTSLRQVYDKGSVLQNQAAYYMALTYAGEKNKTL
                     ALEWLQKIPATAPEHVKIIELEDFLK"
     gene            complement(6810..7367)
                     /locus_tag="VGQ51_10555"
     CDS             complement(6810..7367)
                     /locus_tag="VGQ51_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9037056.1"
                     /translation="MPTHPDQRYIEGLLYNDGSTVDEIYRKYAGAIRKFIIKNGGSGD
                     DAADIFQEALIDLYNQARHKDLQLTCPFEAFLVLVCKRKWYNLQKKRGLHRVTNSTDE
                     VSQIGEDPFARAEQLALEESRARLFLEQFARLGERCREILKLSLTGEAQEKIAERLSV
                     TYGYLRKKKSECMATLLSYIQAQNS"
     gene            7448..>9143
                     /locus_tag="VGQ51_10560"
     CDS             7448..>9143
                     /locus_tag="VGQ51_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEV9037057.1"
                     /translation="MKATIICISMLLCLDALSQDTLPPMLHIREEGDSVRLSATLRPL
                     RQMAGAPAAYYSYFWELGDGHFSFDKEPVYVYRDTGVYDVRLYATNNYDDGKAPPTRP
                     RPVRIRKKTISRGAWVSHFFHGNSDIELKLNRYPRPGEDFVAILGYRNQHADSLSGSI
                     VLFYNDRQLERKGFLLAEQRFYNREESGKLETLVARVDRQAAREDDLAFEEHGSMVGA
                     ADGPGGAEVGGDLPLFASETQAMLHSLEDTYASHAVVHFRAIGRGMEQFVFMDMNTLP
                     EMLQDTNATVGISAMLVPDDPSLPPQLYAMDMQVVSSHDPNRMLVKSRRINYRFFKQK
                     KELLYRVEFQNTGRGPAHTIKIALALPPQLDPESIRLNAMSPVCARCGSGNLGQSCID
                     TLRRGDSIYYTFNNIYLPGIQQEGVADKDSTMGFVEYAVRFRKKPKKIPFGTQAAISF
                     DRQAPVLTNKAPARFIKGLSPGIMVGYSALPANGGYTASGPLQFGYVLAPYAPDRPYF
                     QAEAWVGFLQQDAFTSTVVKDNSDTLIGGLPFIITGRQTRTTVNRNSFEITPLHFRY"
BASE COUNT         2063 a         2032 c         2776 g         2272 t
ORIGIN      
        1 ttccctggaa gaactcctcc acagggacgt agatctggta actgaaaaaa cgataaagaa
       61 cccctatttt cttcaaagtg ttaacgacca taagttaaag gtgttatgag cattgaagaa
      121 aaaaagttat tggtggacat caaggaagct attttgtcta ttgacgatca tttagagggt
      181 aagaggctat tgatgagttc aggtcaaaca aaactaaacg cagggctgtg gagagggaac
      241 ttgagattat tggggaggcg ttgagcaaat tacttaagat taatcccggt attgagattt
      301 ctcacacacg acagatagta agccttcgga acaaggtcat ccatgcatat gatgcaatag
      361 atgagacact catttggaaa attataatgg tcgatattcc ggtgttgctg caagaggtga
      421 cagaattgat ggagggttga taggattggt aagcgcatgc ggaatcgctg ttgccgtgtc
      481 atttttcggc gggatttttc ttgcctgcgg tcagcttttc aactgttcca ttcgcatgtc
      541 actttcgata ccggagcttc ttttcggcgg gttataaata agccagcgtt tgccgggctt
      601 catttggagt ttttggatga ggggagtcat cgcattgaga taaatcgaat atacaaaaaa
      661 agtaatggaa aaaattctgg tgaccggcgg gaccggctcg ttgggttcgg aggtagtaag
      721 acagttgatc tctttgggtt gtaatgtagg tgttttcacg agtcggcgcg atccgcaagt
      781 gccggatggg gtcggggttt ttcatggaga cctgtcaacg ggggaaggtt tggcagaggc
      841 attggcgggg gtgaggacgg ttattcactg cgcgagtaat ttcagctctt ttaaagatac
      901 ggatatcggc ggaacgggta aactcctgga tactgttgat acgtcgcggc cgccacatgt
      961 ggtatatatt tcgatcgtgg gcatcgggaa aagcgtctat ccctattatg tggcaaaacg
     1021 ggccgtcgag gaaatgttgt tgggaagcgg tgtgccatgt accattttga ggactactca
     1081 atttcatgat tttgtcctga acatgatcag aactgttttg gagagtgcgg gcgaaattgc
     1141 gctggtgccg gatgggatcc ggtttcagtc tgtcgatatt agggaggttg ccggcgagtt
     1201 gatccggatc ggtggcgcga aacctgccgg gcttcttccg gatttcggcg ggccggaggt
     1261 tctccggttt gaagatatgt tcgaagaata tgtcgggatg tgcctgcccg gcagagagtg
     1321 gaagatcgct cctgtcgcgg gcatgcgtta tgataatttt cggacgggta taaatattgt
     1381 tcccggtcaa cggagtggta agatcacctg gaaggaattt ttgagatcga aattttaggc
     1441 ggaggaatgt gggggctcgg gtgaatgtgg ggggggggtg ggggcgaatg cgaacggcgg
     1501 atgacggcgg gggcggtgag ctttcagacg atccgcgcgg cattggcgag tccgctaagg
     1561 gcgttgcgta gcgagtaaaa ttgcgtagcg agtaaaatgg tatctttagc ggacaatgcc
     1621 ttttcgatga aaaaagtttt ttttgtcttg ctgatcgctg cgtattttgg agttcacgcc
     1681 caggcgccgg cccgctaccc tttccaggac cgcgacctgc cggtggaaaa gcgcatcgat
     1741 aacctgcttt cgctgctcac catcgatgaa aagatcacct gtcttagcac gaatcctaca
     1801 atacctcgtc tcggattgaa aggcaccggg catagtgagg ggttacatgg gcttgccctc
     1861 ggggtcccgg gtggttgggg gaggaagaca cccgttgcta ctaccacttt cccgcaatca
     1921 tacggtatgg cggagacctg ggacacggcg ctgatccggc aggctgcagc catcgaagga
     1981 tatgaagcgc ggtataccaa tcagaccctc gggaagggcg gtcttgtcat ccgggccccg
     2041 aatgcagatc tcggccgcga cccacgctgg gggaggaccg aagaatgcta cggtgaagac
     2101 gccttcttca atggaacgct ggtgcaggca tatgttcgcg ggttgcaagg tccagatccg
     2161 cgttattggc aggctgcttc gctgatgaaa cattttctgg ccaacagcaa tgagaacagc
     2221 agggatagca gttcgtccga ttttgatgaa cgtctttggc gcgagtacta ttccgtgcct
     2281 tttcgtatgg gcgtcgaagc aggctcccgc gcgtatatgg cctcctataa caaagtgaac
     2341 ggtattccgc aggccgtgaa tccgtcccta agaaaggtca ccgtcgatga atggggacag
     2401 gacggcatca tctgtaccga cggcggagct taccggatgc ttttgacagc tcaccattat
     2461 ttcaagacgc cggaggaggc tgctgcggcc tgcatcaaag ctggtatcaa tcagttcctc
     2521 gacaactacc gcgcaggcgt caagggcgcg ctggaacagc accttcttac ggaaaaggac
     2581 atcgacacgg cgcttcgtgg cgtctgccgg gtgatgatca ggctggggtt atgggatgac
     2641 tcaacgctcg tcccttatgc ttcgatccgt ggcggcgaac ctccttttat gaacgcccgg
     2701 aacaaacaat ttgtccgtct gctgacgcaa aaatccatcg tattgctgaa gaacaccgac
     2761 catttcctgc cactggacaa agccaagctg cacagtatcg cagtcctcgg ttccagaagc
     2821 gagcaggtga tcggcgactg gtatagcggc tctccgggtt atgccgtgag ccctcttcag
     2881 gggatcatcg acaaattggg cgccggcttc agcgtgaatc atactacgaa taacgatctg
     2941 gccgtcagcc tggcgaaaac gtcggatgtc gctatcgtgt gtgtcggcaa ccatcccacc
     3001 ggtggagaga ataagggctg gaaacaggtg tccgtaccga gtgaaggacg cgaagcggta
     3061 gaccgggaac acatagatct cgattccgcg caggagagtc ttgtagaacg ggtcatggcc
     3121 gtcaacccgc ataccatcgt cgtgctcatc agcagctttc cgtattcgat caattggatc
     3181 gaggagcatg tgcctgcggt gttgcatatg acgcagaata gccaggaatt gggcaatgcg
     3241 cttgcagacg tgcttttcgg tgatgtggat cctggcgggc ggctcgttca gacctggccg
     3301 aagtcactgg atcagctgcc gccgatgatg gattatgata ttcgtcatgg aaggacgtat
     3361 atgtacttta aaggtgagcc gctgtatcct ttcgggtatg ggcttagcta tactaatttt
     3421 cggtatggga atttgaggct cagttcgccg gcgatgcgcg atgccgtgga tatttctgtg
     3481 gatgtaacca acacgggtga ccgggaaggg gatgaggtcg ttcagctata tgtgagccat
     3541 gaagggtcca gggttgcccg cccggtcaaa gagctgaggg gcttttcgcg tattacgctt
     3601 cggcccaatg agacaagggc ggtggtgttc cggcttacgc cgaaggatct ggcttattgg
     3661 aatgtggcgc gtcaccggtt tgaaacggag aaagatcaga tcagggtggt gctgggaggg
     3721 tcgtcggcgg atgaaagggc gacggcgagg tttaaggttt tatgaggtaa aataatccta
     3781 tatttaaggc agaacatcct gtcatgtact atctttttta cggcttcctc tacctcctgt
     3841 cgttgcttcc catgcgtctg ctctacctgg tgtcggatgc gatctatggg atcgtctatt
     3901 acgtgttcgg ctaccggaag aagatcgtga tgaacaatct gcggcaggcc ttccctgaaa
     3961 agaccgaagc ggaatgtacg cgtatcgcga agaaattcta ccggaatttt atcgactctt
     4021 ttatcgaggt gatcaagctc gtgtccgcga gcgatgcctg ggtgagcaaa cgatttacag
     4081 ccgacgtggc tgtgctggat gaattgtacg cgacaggaaa ggcctgtcag ctgcaccttg
     4141 gacatacctt taattgggag tgggggcagc tcgcgctgac gccattgacg aaatacaaga
     4201 tccttgtcgt gtatatgcct atcagcagta agatgctgga aaagctattt tacaggcttc
     4261 gcatacgcag cgggaatgtc tttttgccgg cgccgaaaat gcgtgaggcg atggtacagt
     4321 atcttcccac tcaatacctg ctgggattgg ttgcagatca atgcccgggt aatatgcaga
     4381 ctagttggtg gatcgatttc atgggtcgtc ctgcaccgtt tgcgagcggt ccggagaagg
     4441 gagcgcgcgc gggtcaactt ccggtggtat tcgcctccat cgaaaaaccg cggcgcggat
     4501 attatcatgc tacagtccaa ctggcatgtc ccgacgcttc gcaattgccg gaaggcgagc
     4561 tgacacgaat ctatgtccgg tatatggagg acgtgatccg ccgccggccg gagatgtggc
     4621 tatggagtca ccgtcgatgg aaacatggct ggaaagaaga gtatgagccg atgcggatcg
     4681 acgagggcag ggtttcaaat acttataggt ggaaagagag tgacgctgtg aaatagaaga
     4741 gattggaagc gagctcggag cctgcaacga tgttccgggg gaggacgtag gcgggtgtca
     4801 atagtaattt cattttgccg agaccataga cgaccgggca gctgagctca taggcgagta
     4861 tattgaattg ttcgctgctg gtggtgatct gttgttgctg tgtagggagg aagaggaagt
     4921 atttttgttc gtagtacgtg gtggtgaagt tttgcgtgcc ggcggagatg gttgccgttg
     4981 ggtcgaagac gaggacgcct tttttggttc cgacattgag gatacggatg atatgatcga
     5041 gaccggtctg gagactgtag tctatccggt cgctccagcg ggcgctgccg gtgagggtga
     5101 tattggcgac attattcagc cgggtgaggc tggcggatgc catttccttt atcaccgact
     5161 cgaccagatc gatgttttgt tcatacagga agcgcgagac ggagagatta cctgcccagt
     5221 ggttggaact gttgcctcct ccgaagttgt agccggcttc gagaagtgtc gcggcatatt
     5281 gcgattgctg gctgttttcg aggaagacga aggtggagtt gagatagagg ccatcgttga
     5341 gcgagaggcc gataaagggg cagatgcctt tgcttcggag gctgtctata cgaccgtagt
     5401 agttgagacc ggaattatag ataatgccgg cgttgaggtg gaggatggtg gagtcctggg
     5461 cgtgggtgag ttgcgcggtt aagacgaaag cgaggatcag atggaggcgt ggcatgagaa
     5521 gagttttttg gtaagccggg gcagcttgcg ctaccccggc cgttgaggga ttattaatgc
     5581 acagacacat tcgtggaagc gctggtggat acaccgacgc tctttttagt tgcagacgct
     5641 gaggtcgaag attgcatgtt ggtcgaggcg ccagtggatc ttgcggcttt gacggtagaa
     5701 gccgaagtct gggaggcttt ggaggcagcc tgggccgccg tttgggtgga tttggcggca
     5761 gtatgcgcag tggttgctgc ggccgtatgc gcggtggttg ctgcggtggt tgtggtcgct
     5821 gcggcacttg ctctgagcgc attgcttact gcgggcgtat taacgctggc ggcggagctc
     5881 agccgggcat tggcgacggc gcctagacgg atctgagcaa acgagtgatt ggaaatacct
     5941 aaaaaggcga taacaattgt tgcaataata gcgcgtttca taatactgat tttgatggtt
     6001 aagaaatcgg gcctgagccc tttcgttagt atatccatcc aatcaggatc ttgttacccc
     6061 tcttgcgacc gctattttaa aaaatcttct aattcaatga ttttcacgtg ctcgggcgct
     6121 gttgcgggta ttttttgcag ccattcgagc gccaatgttt tgtttttctc accggcatat
     6181 gtcaacgcca tataataggc cgcctggttt tgcaggacgg atcctttgtc atacacttgt
     6241 cgtaaggacg tacgtgccac gtggaggtct cccgtatgcc aggccgctac tccgcgatag
     6301 aaaagggcca gttggctact gctgtctgcg atgacagcct ggttcaaaat gggcagcgct
     6361 ttatcgaatt gcttttcgtt gaaaaaggtg gacgcctttt gaaggagggt gtcggtttga
     6421 ttccctcttt cggtaatgcc gatcatgctg gtttcgccaa gctggtcgag tttacgggtg
     6481 gtctcggatg gccacgatat gatgatgaca gctatgatac atgcggcggc ggcgactgta
     6541 gaaagaaagc ggagggtcgg ggcctttcgg atgcgggtct gctgaaaaaa ttgttcgttc
     6601 aaccgtgcag ttcgttgctt tagcgcgtcg agggtcggat ccccgggcag ccgctcgtgc
     6661 aggacggcct tcaattcccg gtatcgttcg acttccgtgg caagagcggt atcggtgcgc
     6721 atagccgctt cgaactggcc tgcctcttcg gggctcagct caccgtcgac ataccggatg
     6781 atccattcat tatcataact gtccatagct taggaattct gtgcttgtat ataggataat
     6841 aacgttgcca tgcattcgga tttctttttc cgcagataac cataggttac acttaaccgt
     6901 tcggcgatct tttcctgagc ctctcctgta aggctgagct ttaatatttc ccggcatctt
     6961 tcgcccagcc gggcgaactg ttccaaaaaa agacgggcgc gggactcctc gagtgcgagc
     7021 tgttctgccc tcgcaaatgg gtcttcgcct atctgagata cttcgtctgt ggaatttgtt
     7081 acccggtgga gccctctttt cttttgaaga ttgtaccact ttcttttgca gacaaggacc
     7141 agaaatgctt cgaagggaca ggtgagctga agatccttat gccgcgcctg attgtacaga
     7201 tcgatcagtg cttcctggaa gatatcggct gcgtcatccc ccgagccgcc atttttaata
     7261 atgaatttcc ggatggcgcc ggcatatttc cggtagatct catccacggt ggatccgtcg
     7321 ttgtataaca ggccttcgat atatcgctga tccgggtgag tcggcattcc aggattttgg
     7381 acgaatatct aaaaaaataa tggaataggg ggtaacaaaa caaacggcag gttgatatct
     7441 ttaaggtatg aaggcgacga tcatatgtat atctatgctc ctttgtctcg atgccctttc
     7501 gcaagacact cttcctccga tgttgcatat ccgggaagaa ggcgatagcg tcaggctatc
     7561 ggcgacgctg aggcctttga gacagatggc aggggcgcct gctgcgtatt atagctattt
     7621 ctgggagctc ggagatggac atttcagctt tgacaaggaa ccggtttatg tgtaccggga
     7681 tacaggggtt tatgatgtga ggctttacgc taccaacaac tacgacgacg gcaaggcgcc
     7741 gcccacaaga ccgagaccgg tgaggatcag gaaaaagacg atttcgcgcg gtgcatgggt
     7801 atcgcatttt tttcatggaa atagcgacat agaattaaaa ctcaaccggt atccccggcc
     7861 gggtgaggat ttcgtggcga tcctgggtta tcggaatcaa catgctgata gcttaagtgg
     7921 aagtatcgta ttgttttata atgaccggca gctggagaga aaagggtttt tgcttgcgga
     7981 acagaggttt tataataggg aagaaagtgg aaagttggag actttggtgg cgcgggtgga
     8041 tcggcaggcg gcgagggagg atgatttggc ttttgaagag catggatcga tggttggtgc
     8101 ggcggacggg ccgggcgggg cggaggtcgg cggggatctg ccgctgtttg cgagtgagac
     8161 acaggcgatg ctgcattcgt tggaggatac ctatgcgagc catgcagtgg ttcattttcg
     8221 cgcgataggc aggggcatgg agcagtttgt gttcatggac atgaacaccc tgcccgagat
     8281 gttacaggac accaatgcga cagtcggaat ctcggcgatg ctggtgccgg atgatccaag
     8341 cctgccgccg caattgtatg cgatggacat gcaggtggtt tcatcacatg atccgaaccg
     8401 gatgctggta aaatcacgcc gcatcaatta ccgttttttc aaacaaaaaa aggaactgct
     8461 ctaccgggta gaatttcaga ataccggccg ggggcccgcg catactatta agatcgcttt
     8521 ggcgctgcct ccccagctgg acccggagag catccggctc aatgcgatga gccccgtctg
     8581 tgcacggtgc ggatccggta atttgggtca gagctgtatc gataccctgc ggcgcggcga
     8641 ttccatctat tatacattca acaacatcta tcttccgggg atccaacagg aaggtgtagc
     8701 cgacaaggac agcaccatgg gttttgtgga atatgcggtg cgtttcagga agaagccgaa
     8761 gaagataccg tttggtactc aggcagcgat ctctttcgat cgccaggccc cggtgctaac
     8821 caataaggct ccggccagat ttataaaagg tctttcgccg gggatcatgg tggggtatag
     8881 cgccttgccg gctaatggag gctacaccgc gagcggaccg ctgcaattcg gttatgtact
     8941 cgcgccgtat gctcctgaca gaccctattt tcaggcggag gcatgggtag ggtttcttca
     9001 acaggatgct tttaccagta cagtggtaaa ggacaacagc gacacgctca tcgggggact
     9061 gccttttatc attaccggaa gacagacgcg aacgacggtg aaccgcaata gttttgagat
     9121 cacgccgctg cacttccggt ata
//
LOCUS       DASYSV010000351         3887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1222155, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000351 DASYSV010000000
VERSION     DASYSV010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222155"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..2054)
                     /locus_tag="VGQ51_10565"
     CDS             complement(258..2054)
                     /locus_tag="VGQ51_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9037058.1"
                     /translation="MAQEVKKKKIFNFSLLRRVFHYAAPYKRRMYSSIALAIVLAILS
                     PLRPWLIQLTINGYIRSGAEQAIILITIWQIGLLLLETASRFYFGFITAWLGQTVVRD
                     LRVDTYKKIIGLNLSQFDKTPIGTLTTRTINDIEAINDIFSDGLIPLLADLLSIVSIL
                     LFMFITDWRLTLVCLAPFPFLLLATWMFKESVNKSFIRVRNAVAALNAFVQEHITGMA
                     IVQAFAAEKREALKFNKINREHRNANINAIFAYSVFFPIVEIILALSTGLLVWWCAAP
                     AMKLPPDQARLLAGEIVSFYLYLNLLFRPLRVMADKFNVLQMGMVASERVFRVLDNDD
                     YIKTQGNFAPTRLRGKVEFDKVSFAYIDDRYVLKNISFTMQPGQTIALVGHTGSGKTS
                     IISLLNRLYHIQEGEIRIDDVRIEDYRLDALRKSIGVVLQDVFLFSGSVVDNITLRND
                     NITMERVIEAAKLIGMHDFILRLPGGYHYNVMERGSSLSLGQRQLLSFIRALLYDPSI
                     LILDEATSSVDTESEQLIQEAIDKLIAGRSSIIIAHRLSTIRKASQIIVLDKGEIREI
                     GTHDQLLAKGGFYAKLHEMQFSKHSSAERKAS"
     gene            complement(2112..>3887)
                     /locus_tag="VGQ51_10570"
     CDS             complement(2112..>3887)
                     /locus_tag="VGQ51_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037059.1"
                     /translation="AAPYETGMILVDCFNYAPSENIGVRPSRLLLTRRYCEMHPSLIF
                     NYLSIHPGLPFEDSLIIVAGHHNIGQLYDFAAAGNELAYHIRNSRDSLVHTVAMLAGS
                     RSGRLYFPFLDNLVRGTITTDDIDKVKDDDLQYYRLLVKTRLDYAARLLPPACDTPLE
                     MNALTEMLEKKGKQVFVGEINALHTVENPAVRFKILDPLTPEELYYLVVLSEDEIYTS
                     SYLGVYDRIFQRMKAPYGDSLLMAVHGDYFRKWIKMAAAYNKLEHFLGTMDKQNAGTI
                     MKSFVIHLENAGEEEAVDVADSYSSIVEKSPALAHFILDEVRSNYDKNVATGNKKGTI
                     IYHLLETLFESADTNSKIDLSASLGIPPVYSVDHQSLTDDSGRVIEQVFFYGDKDKDG
                     QNSYADFMSLFHTRPGTKPEWKITENSQWTTITSVRGKPVLIFANKPLLGEDDPDAKA
                     QKALDDYLFGRHLRPTIVIHRGHSYHVKYTIEQMPATARIVVLGSCGGYNNLSEVLKI
                     SEDAHIISSKQVGTKTVNEPILRAINNTLLAGRDIEWLPMWRELEASFQKDAAAREKF
                     DDYIPPYKNLGAIFIKAYRKAMDSE"
BASE COUNT          915 a          947 c         1132 g          893 t
ORIGIN      
        1 cgatgtgttc caaaatggcg tcggaagggt tgaacttctg cgcataggac atattagaaa
       61 aaaacaccaa aaacaagctg aaagccgcga ccaataaaga tttgaagctc ctggatgcca
      121 tacctgtccc aaaatttggc gcaaagatac ggggggaggg gcagaatatg aaaattcggt
      181 taaagaaaat cgatccgcgg aggaacggat ttgcataggc gcacggggcg gcgcgtgcgc
      241 ggttggttac cacgggctta ggacgcctta cgttcggcgg agctatgctt gctgaattgc
      301 atctcatgca atttggcata gaaaccacct ttggcgagca gctggtcatg agtcccgatc
      361 tcccttatct cacctttgtc gagaacgatg atctggctgg ccttgcgaat ggtggaaagc
      421 cgatgggcga tgatgatgga gctgcgcccg gcgatcagct tgtcgatggc ctcctggatc
      481 agctgctcgg actcggtgtc cacagaagac gtcgcctcgt cgaggatgag gatcgaaggg
      541 tcgtagagca gcgcgcggat gaaggataac agctgacgtt gccccagcga aagcgagctg
      601 ccgcgttcca tgacattgta atgatatccg cccggcagcc gcaggataaa atcatgcatg
      661 ccgatgagct tcgcggcttc gatcaccctt tccatggtta tattgtcatt gcgaagggta
      721 atattgtcga ctaccgatcc ggagaaaaga aagacatcct gcaggaccac gccgatactt
      781 ttccgcaggg cgtcgagcct gtaatcttcg atccgtacgt cgtcgatccg gatctccccc
      841 tcctgaatat gataaagcct gttgagcagg ctgatgatgg aagtcttacc gctacccgta
      901 tgtcctacga gtgcgatggt ctgaccgggt tgcatcgtaa aggatatatt cttcaggaca
      961 tagcggtcgt cgatataggc gaaacttacc ttgtcgaact ctactttgcc gcgcaaacgg
     1021 gtgggagcaa agttcccctg cgtcttgata taatcgtcgt tgtcgagaac gcggaagacg
     1081 cgctcgctgg ccaccatgcc catctggagg acattgaatt tgtcggccat cacacggagg
     1141 ggacggaaaa ggaggttaag atagagataa aaagaaacga tctcgccggc aaggagacgg
     1201 gcctggtccg gaggcagctt catcgcggga gcggcgcacc accagacgag caggccggtg
     1261 gacagcgcca ggatgatctc cacgatgggg aagaataccg aataggcaaa gatggcattg
     1321 atattggcat tccggtgttc gcggttgatc ttgttgaatt tgagggcctc gcgtttttcc
     1381 gccgcgaagg cctggacgat cgccattccg gtgatatgct cctgtacgaa ggcgttgagt
     1441 gccgccacgg cattgcgtac acggataaag gacttgttga cagattcctt gaacatccag
     1501 gtggccagca gcaggaacgg gaacggggca agacagacca gggtcagccg ccagtccgtt
     1561 atgaacatga acaacaggat ggatacaatc gataaaagat cggcgagcag ggggatgagg
     1621 ccatcggaaa agatatcgtt tatcgcctcg atgtcattaa tggtcctggt ggtcagcgtg
     1681 ccgatgggcg ttttgtcgaa ttgggaaaga ttgagaccga tgatcttttt atacgtgtcg
     1741 acacgaagat cgcggacgac ggtctgaccg agccatgccg tgataaagcc gaaatagaat
     1801 cgtgatgcgg tctccaaaag gagaagacct atttgccaga tggtgatcag gatgatggcc
     1861 tgctctgcac cggaccggat atagccattg atcgtcaatt ggatcagcca ggggcggagc
     1921 ggagagagga tagcgagcac gatggccagc gcgatggacg agtacatgcg gcgcttatac
     1981 ggggcagcgt agtgaaaaac gcggcgtaac agactgaaat tgaatatctt ttttttcttg
     2041 acttcttgtg ccatgctacc caaatttaca aaattgagga gaaagccgcg ggcgacagtg
     2101 gcgagcggcg attattcgct gtccatggct ttacggtaag ccttgataaa gatggcccca
     2161 aggttcttgt aaggcgggat ataatcgtcg aatttttccc tcgcggcggc gtccttttgg
     2221 aaactggctt ccagctcgcg ccacataggc agccattcga tatccctacc agccagcagc
     2281 gtattgttga tggcgcggag tatgggttcg ttgacagtct tggttccaac ttgtttggag
     2341 gagatgatat gcgcgtcttc gctgatcttc agcacctcgc tgagattgtt gtatcccccg
     2401 catgatccga ggacgacgat acgggccgtt gcaggcatct gttcgatggt atacttgaca
     2461 tgatagctat gtccgcggtg gatgacgatc gtgggtcgca ggtgacggcc gaagagataa
     2521 tcatccaacg ccttctgggc tttcgcatcc ggatcatcct caccaagcag cggcttgttc
     2581 gcaaaaatga gcacgggttt gccccgaacc gaggtgatgg ttgtccactg cgaattctcg
     2641 gtgatcttcc actcgggctt cgtacccggc cgcgtatgga aaagggacat gaagtcggcg
     2701 taggaattct gtccgtcttt gtccttatcc ccatagaaga acacctgttc gatcacccgg
     2761 ccgctgtcat cggtcagcga ttgatgatcg accgaataga ccggtggtat gcccagggag
     2821 gcggagagat cgatcttgct gttggtgtcg gcggattcga acagggtttc gaggaggtga
     2881 tagatgatgg tccctttctt attaccggta gctacattct tatcgtagtt ggacctcacc
     2941 tcgtccagga tgaaatgggc cagggcagga cttttctcta ctatactact ataggagtct
     3001 gccacgtcca cggcctcttc ttcacccgca ttctcgaggt ggatgacgaa ggatttcatg
     3061 atcgtgcccg cattctgctt atccatcgtg cccaggaaat gttcgagctt gttgtacgca
     3121 gcggccatct ttatccattt ccggaaataa tcgccatgga ccgccatgag caggctgtcg
     3181 ccataagggg ccttcatccg ctggaatatg cggtcgtata cgccaagata gctggaagta
     3241 taaatctcgt cctcgctgag caccaccaga taatataact cttcgggggt cagcggatcg
     3301 agtatcttaa accggacggc cgggttttcc acggtatgga gggcatttat ctcgcccacg
     3361 aagacctgtt ttcctttctt ttccagcatt tcagtcaggg cattcatctc aaggggggta
     3421 tcgcaggcag gggggagcag ccttgcggca tagtccagac gcgtttttac gagcaaacga
     3481 taatattgaa gatcgtcatc ctttacctta tcgatatcgt cggtcgtgat cgtacctctg
     3541 accaggttgt ccaggaaggg gaagtagaga cgcccgcttc tgctaccggc gagcattgcg
     3601 accgtatgga cgagcgaatc cctgctgtta cggatatgat aagccagctc attgccggcg
     3661 gcggcgaaat cgtacagctg cccgatattg tgatgtccgg cgacgatgat caggctgtct
     3721 tcgaacggga ggcccggatg tatagacaag tagttgaaga tcagggacgg gtgcatctcg
     3781 caatagcgcc gtgtcaggag gagacgggaa ggtctgaccc cgatattctc cgaaggcgca
     3841 taattgaagc agtcaaccag gatcatcccg gtctcatagg gagcggc
//
LOCUS       DASYSV010000352         7517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1477027, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000352 DASYSV010000000
VERSION     DASYSV010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477027"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /gene="mqnB"
                     /locus_tag="VGQ51_10575"
     CDS             complement(<1..624)
                     /gene="mqnB"
                     /locus_tag="VGQ51_10575"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HEV9037060.1"
                     /translation="MHILLAAATTFEIQPTIEGGWSQVLITGVGGVATTWSLMRQIGI
                     HRPDLIVQAGIGGCFTDAKPGTVFAIREDELADLGVWENESFLTLSDLGLADVNRLPN
                     PYHDLLAFTGLPEASALTINEITTDPRRIAWYRQNRDAVVESMEGGPLHYVCLRENIP
                     FLQLRSISNTVGVRDKTKWDIRLAITRLNEQLTRILHRLSSADPSLLT"
     gene            674..1084
                     /locus_tag="VGQ51_10580"
     CDS             674..1084
                     /locus_tag="VGQ51_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEV9037061.1"
                     /translation="MVYLTRLEHFNAAHKLYNPGWSREKNEDVFGKCANENWHGHNYE
                     LYVTIKGEPNPDTGFVFDVKRLGEIIREHIIGKVDHKNLNLDVDFMQGKMCSTENLVI
                     GIWQQLQQHLPAGVCLHSIKLYETPRIYVEYFGE"
     gene            1095..1505
                     /locus_tag="VGQ51_10585"
     CDS             1095..1505
                     /locus_tag="VGQ51_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEV9037062.1"
                     /translation="MKKVAVFRREHFNAAHRLFNPAWDDRKNTEVFGKCSNPHYHGHN
                     YELVVKVTGVPDPDTGYVMDLKVLSVITAEEVLARFDHKNLNLDTEFFHDLNPTAENI
                     AIVIYDLLRARIDRELELKVRLYETERNFVEYPA"
     gene            1557..2153
                     /gene="folE"
                     /locus_tag="VGQ51_10590"
     CDS             1557..2153
                     /gene="folE"
                     /locus_tag="VGQ51_10590"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV9037063.1"
                     /translation="MAYSKIEHYDEEITTALTKSYRDSLHLLGEDAKREGLLKTPERV
                     AKAMQFMTQGYSLDAHAILNSAKFHEEVSEMILVKDIELYSLCEHHMLPFFGKAHVAY
                     IPNGWITGLSKIARVVDVYARRLQVQERMTTQILHAIKETLNPLGVAVVIEASHLCMM
                     MRGVQKQNSVTTTSAFWGEFEKNETRSEFTKLISSNLH"
     gene            2261..3151
                     /gene="fabD"
                     /locus_tag="VGQ51_10595"
     CDS             2261..3151
                     /gene="fabD"
                     /locus_tag="VGQ51_10595"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV9037064.1"
                     /translation="MKHAYVFPGQGSQFPGMGKNLYDNSAFAKKLLEQANDILGFRIS
                     DIMFSGSEEDLRQTKVTQPAIFLHSVIAFKGIENSRPEMVAGHSLGEFSALVACGVLS
                     FEDGLQLVAARAGAMQHACEKEHSSMAAILGLEDEKVEEICARVSGETGETVVPANYN
                     CPGQLVISGGVRAVEAACQQMKTAGAKRALILPVHGAFHSPYMEPAADELTGAIEKTT
                     FYNPTCPVYQNVAAKAVVAKDEIKKNLIDQLTGAVKWTQSVRAMITDGASHFTEIGPG
                     KVLQGLIQKIDKTVQVDGVS"
     gene            complement(3186..3632)
                     /locus_tag="VGQ51_10600"
     CDS             complement(3186..3632)
                     /locus_tag="VGQ51_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9037065.1"
                     /translation="MDSIAIRRAVREDCPRLLELIRELAEFERAPQEVTVTPQHFEDS
                     GFGPHPVWWAFVVTINGRIEGFALYYIRYSTWKGQRLYLEDLIVTEEMRGKGLGKLLF
                     ERLLQEVKDSGFTGMVWQVLDWNEPAINFYKKYGAQLDAGWINCSL"
     gene            complement(3636..4628)
                     /locus_tag="VGQ51_10605"
     CDS             complement(3636..4628)
                     /locus_tag="VGQ51_10605"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEV9037066.1"
                     /translation="MSKKLTAAITAIGGYVPEDRLTNADLEKMVDTNDEWIRTRTGIE
                     ERRILREPGKATSDLCVPVVQEICRKRGIDPMEIDILIVATVTADMLFPATANIVCDK
                     VGAKNAWGFDMSAACSGFLYSLTTGAMFIESGRAKKVVVVGADKMSAIVDYSDRTTCI
                     IFGDGAGGVLLEPNYDGYGILDSLLKSDGSGRNHLHMKGGGSLHPSSFETVAAKEHYI
                     YQEGQAVFKFAVKGMADISAELLERNNLKSDDIAWLVPHQANKRIIDATAHRMGLPEE
                     KVMINIQRYGNTTAGTIPLCLWDWEDRLRKGDNLVLAAFGGGFTWGATWVKWGY"
     gene            4727..5314
                     /gene="ruvA"
                     /locus_tag="VGQ51_10610"
     CDS             4727..5314
                     /gene="ruvA"
                     /locus_tag="VGQ51_10610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV9037067.1"
                     /translation="MIAFLRGNFVYKSPALVHLEAGGVGYELQISLNTYTSIQNLESG
                     ILLTYLHIREDAHILYGFIDKAEKDMFIHLISVSGVGASTARMMLSSLKPEEITKAIL
                     QGNARQLESIKGIGKKSAERIILELRDKVGKGREETLNISSVTNNTVEQDALNALITL
                     GIARPAAENAIKKVLKEGAGAEKIEDIIKNALKIL"
     gene            5356..>7517
                     /gene="sprA"
                     /locus_tag="VGQ51_10615"
     CDS             5356..>7517
                     /gene="sprA"
                     /locus_tag="VGQ51_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HEV9037068.1"
                     /translation="MTRHSILLILAMAALTAVAVVSSMTVAAHPFQQPGRVDTGSNPQ
                     LQRAADSMRRQRDSLRRLQGDTAKKDTLHWPIEDRRGDRFTNPNLNPWYLRNPPNLRD
                     SIVYDPVTNQFYIMEKIGNQYYRKPTYLTYDEMMKMVGDSAEDAYFRKRADAMSALNR
                     AMRPKLYVGNNLFNRIFGTGKIDIRPQGTVDIMAGYQGQNIQNPTLPESARRTGGFDF
                     NMNANLQVVGNIGDKLKLPISYNTLANFDFENQLKLDYTGSSDEIIKKIEAGNVSFTT
                     PSTLMTGAQSLFGIKTKLQFGKLTVTAVLANQRSQKQSVNSSGGASVTTFSFKADDYD
                     ENRHFLLAQYFHDNYNAAMSRIPLVTSQVQILRMEVWVTNRNGIDTGSRQIVGLMDLG
                     ETKPYNPNIRPLTNQPYPSNDNNSEYRSIINDPSSRSPTTAASKMNSLGLSQVQDFEI
                     VYARKLNPTDYYFNPQIGFISLNQTLQANDVLAVAYQYSYNGHIYQVGEFSTDVPPDT
                     TSGTFGGSSKVLYLKLLKATSQRTNLPIWNLMMKNIYSLKTSVGGYLSNIQQQGFQFN
                     VNYDQPSLGTKRYLPEGPRANLPLITVLGLDRLNAHNDPGPDGVFDYIEGLTVMSSQG
                     RIIFPVLEPFGRDLDTLAFAGQPQSVKNKYVFYQLYDTIKAVAQTYANVDRYVLAGSA
                     KGQSTSSLSLNAYNVPPGSVVVTAGGQTLREGVDYVVD"
BASE COUNT         1852 a         1961 c         2019 g         1685 t
ORIGIN      
        1 tgtaagtagg gaggggtcgg ctgacgacag tcggtggagg atacgggtca attgttcatt
       61 cagacgcgtg atcgccagac ggatatccca ttttgtcttg tcgcggacgc ctacggtatt
      121 ggatatggac ctcaattgca aaaaggggat gttctcccgc agacacacat aatgcagtgg
      181 gccgccttcc atggattcga caactgcgtc cctgttttgc ctgtaccagg caattcgccg
      241 cgggtcggtg gttatttcgt tgatagtcaa agcacttgct tccggtagcc cggtaaaggc
      301 gaggagatcg tggtaaggat tgggcagtct gttcacgtcc gctagtccga gatcggagag
      361 agtgagaaaa ctttcatttt cccatacgcc gagatccgcg agctcgtctt cgcggatggc
      421 gaatacggtg ccgggttttg catccgtgaa acagccgcca atgccggcct ggacgatgag
      481 atccgggcga tggatgccaa tttggcgcat tagcgaccag gtggtggcga cgccgccaac
      541 gccggtgatg aggacctgag accagcctcc ctcaatagta ggttgaattt caaacgttgt
      601 tgcggccgcc agcaggatat gcatggtgca aatatccggc tcctatgcat tacctttgcg
      661 gaaatttttt cgtttggtct atttgacgcg actggaacat ttcaatgcgg ctcataagct
      721 gtataacccc ggctggagca gggagaaaaa tgaggatgtc tttggcaaat gcgccaatga
      781 gaactggcat gggcataact atgagctgta tgtgacgatc aagggggagc cgaacccgga
      841 tacggggttc gtattcgacg taaagagatt gggcgagatc atccgcgagc atatcatcgg
      901 gaaggtggat cacaagaatt tgaacctgga cgtggatttc atgcagggta agatgtgttc
      961 gacggagaac ctggtgatcg gtatctggca gcagttgcag cagcatttgc ctgcgggagt
     1021 ttgtctccac tccatcaagt tgtatgagac tccgaggatc tatgtagaat atttcggtga
     1081 ataaataaat gacaatgaaa aaagtagccg tgttccggcg ggaacatttc aatgcggcgc
     1141 atcgcctttt caatccggcc tgggatgacc ggaaaaatac cgaagttttc ggtaaatgca
     1201 gcaatccgca ttatcacggg cataactacg agctggtggt gaaggtgacg ggagtgcctg
     1261 accccgacac agggtatgtc atggatttga aggtattgtc cgtgatcacc gcagaggagg
     1321 tattggcaag gttcgatcac aagaacctca atctggacac tgagtttttc catgatctca
     1381 atcctacggc ggaaaatatc gcgatagtga tctatgatct gttgagagcg aggatcgatc
     1441 gggagctcga gctcaaggta agactatatg aaacggaaag aaattttgta gaataccccg
     1501 cctgaagaca agagaggcgg agacctctcc catacattgg cgtttaaatc catcaaatgg
     1561 cttattcgaa aatcgaacac tacgacgaag agatcaccac ggcactgacg aagagctacc
     1621 gggattcact gcatctgttg ggcgaggatg cgaagcggga aggtcttctc aagactccgg
     1681 aacgtgtggc gaaggctatg cagttcatga cgcagggcta tagcctcgac gcacatgcca
     1741 ttctcaattc ggccaaattc cacgaggaag tgagcgaaat gatcctggtg aaggacatcg
     1801 agctgtacag tctgtgcgag caccatatgc ttcccttctt cggcaaggcg catgtcgcct
     1861 atatccctaa cggatggatc acgggactga gcaagatcgc cagggtagtc gacgtatacg
     1921 ccagaaggct ccaggtacag gaacgaatga ccacccagat attgcacgcc atcaaggaaa
     1981 cgctgaaccc cctgggtgtc gcggtggtca tcgaagcgtc gcatctttgt atgatgatgc
     2041 gcggtgtaca aaagcaaaac tccgtaacga ccacgtcggc cttttggggt gaattcgaaa
     2101 agaatgaaac ccgcagcgag ttcaccaaac tgatctcctc gaatctgcat tgaaatagcg
     2161 accgaacaat aagattttct ttgctgagtc ggtgctttgt ggcatatttg cggtttgggc
     2221 ggacccccgg ttcgccggcc catcaaaaac ttagaccggt atgaagcacg catacgtttt
     2281 cccgggccag ggatcgcagt tcccgggcat ggggaagaat ctctacgata acagcgcttt
     2341 cgccaagaaa ttattagaac aagccaacga tatattggga tttcgtattt cggacatcat
     2401 gttctccggg agcgaagaag atcttcgtca gacaaaggtg acccagccgg caatcttttt
     2461 acattctgtg atcgccttca aaggtatcga gaacagcaga cccgagatgg tggcaggaca
     2521 ttcccttggc gagttctcgg cgttggtggc ctgcggggta ttgagcttcg aggacgggtt
     2581 gcaactggta gcggcccgcg cgggcgccat gcagcatgcc tgtgaaaaag aacattcctc
     2641 tatggcggcc atcctcggtc tggaggacga aaaggtcgag gagatatgcg cgcgtgtcag
     2701 cggcgagacg ggtgagaccg tcgtacccgc caactataac tgtccgggtc agctggttat
     2761 cagcggaggg gtccgtgcgg tggaagctgc ttgccagcaa atgaagacgg cgggcgccaa
     2821 gcgggcgttg atccttcccg tacacggggc atttcattcg ccttatatgg agccggcagc
     2881 agacgaattg accggcgcga tcgagaaaac gactttttac aacccgacct gccccgtcta
     2941 tcagaatgtg gcggcaaagg ccgtggtcgc caaagacgag atcaaaaaga acctcatcga
     3001 tcagctgacc ggggccgtaa agtggacgca gtcggtgcgc gctatgatca ccgacggtgc
     3061 atcgcatttc accgagatag gccccggcaa ggtgttacag ggattgatcc agaagataga
     3121 caagacggtg caggtggatg gagtcagcta aggctaatga ttgtccttga gttgccgttg
     3181 gcaactcaaa gcgaacaatt gatccatccc gcatccaatt gagcgccgta ttttttatag
     3241 aaattgatag ccggttcatt ccagtccagc acctgccaga ccatgccggt gaatccacta
     3301 tccttcactt cctgcagcaa ccgttcgaat aacaatttcc ccagtccctt tccgcgcatt
     3361 tcttcggtga cgatgaggtc ctccaggtaa agacgttgtc ctttccaggt actataacgg
     3421 atataataca gggcaaatcc ttcgatacgt ccgttgatgg tgacgacaaa tgcccaccag
     3481 acggggtgcg gcccgaagcc actgtcttcg aaatgttgcg gtgttaccgt cacttcctgt
     3541 ggggcgcgtt cgaattcggc cagttcacgg atcagttcca gcagacgggg gcagtcttcg
     3601 cgaacggccc ggcggatggc gatagagtcc atagtctagt acccccattt tacccaggta
     3661 gcgccccagg tgaatccacc gccgaaagcg gccaatacga gattatcgcc cttgcgaagc
     3721 ctgtcttccc aatcccagag acataacggg atggtccccg cagtggtgtt cccgtatcgc
     3781 tggatattga tcatcacctt ttcttccggg aggcccatcc ggtgggcggt ggcgtcgatg
     3841 atgcgtttgt tggcctgatg tggaacgagc caggcgatat cgtcggattt gagattgtta
     3901 cgctccagca attcggcgct gatgtcggcc atgcctttca cggcaaattt gaacacggcc
     3961 tggccttcct gatagatata atgctctttg gcagcaaccg tttcgaaaga ggacggatgc
     4021 aacgacccgc ctcctttcat gtgaagatga ttcctgccgc tgccatcgct cttcagaaga
     4081 ctgtccagaa tgccataccc gtcataattg ggttcgagca ggacaccgcc ggcgccgtcg
     4141 ccgaagatga tacaggtggt acggtcacta tagtccacga tggcgctcat cttatcggca
     4201 cctaccacga ccactttctt cgcccggccg ctctcgatga acatggcgcc ggtggtcaat
     4261 gaatacaaaa aacccgagca cgccgcgctc atatcgaagc cccaggcatt cttcgcgccc
     4321 accttgtcgc aaacgatatt tgccgttgca ggaaacagca tatctgctgt gactgtggca
     4381 acgatgagaa tatcgatctc catcggatcg ataccccgtt tgcggcagat ctcctgtacg
     4441 acaggtacgc aaagatccga agtggctttg ccgggctctc ttaatatcct tctttcctct
     4501 atccccgtcc gtgtccggat ccattcgtca ttggtgtcga ccattttttc cagatctgca
     4561 ttcgtcaaac ggtcttcggg tacgtatcct ccgatcgctg tgatcgcggc ggtcagtttc
     4621 ttactcatta taaatttcca atcaggtgaa gaaaagtttt ttgcaaaggt ggggaaaaat
     4681 atccaatgag cacttccggc acgtattttc ccctattttc gtagctatga tcgccttctt
     4741 acgcggaaat tttgtctaca agagtcccgc cctggtgcat ttggaggcag ggggggtagg
     4801 gtacgagctg cagatcagcc ttaataccta tacgagcatc caaaatctgg aaagcggcat
     4861 tctactcact tacctgcata tccgcgaaga cgcgcatatc ctgtacggtt ttatcgacaa
     4921 ggcggaaaaa gacatgttca tccacctgat cagcgtctcg ggggtcggcg cttccaccgc
     4981 ccgaatgatg ttgtcttcgc tgaaaccgga agagatcacg aaggctattc tgcagggaaa
     5041 tgcgcgacaa ttggagagta taaagggcat cggtaaaaag tccgcggaaa ggatcattct
     5101 ggagctgcgc gataaggtgg gaaaaggcag ggaagagacc ctaaatattt catcagtcac
     5161 caacaatacg gtagaacagg acgcgttaaa tgcactgatc acgctgggaa tagccagacc
     5221 tgccgcagaa aatgccatca aaaaggtctt aaaagaaggc gccggggcag agaagatcga
     5281 ggatatcatt aagaacgctt taaaaatctt gtagcctatc ggcgaaactc tacctaacta
     5341 attcttgagg aaagtttgac ccgccactcg attttgttga tcctggcaat ggcagcgctg
     5401 acggctgtcg cggttgtgtc ttccatgacc gtggctgctc atccattcca gcaacccgga
     5461 cgggtggata ccggttcgaa tccacaattg caaagggccg ccgattccat gcggcgtcaa
     5521 cgcgactctc tccgcagact tcagggcgat acggcaaaga aggacactct ccattggccc
     5581 atcgaggaca gacgaggaga caggttcacc aaccccaacc tcaatccatg gtatctcagg
     5641 aatcccccca atttacgcga ttcgatcgtc tatgatcccg tcaccaatca gttctatatc
     5701 atggagaaga ttggtaatca gtattatagg aaaccgacct atctcacata tgacgaaatg
     5761 atgaaaatgg tcggagattc ggccgaagac gcctatttcc gcaaacgggc cgatgccatg
     5821 agcgccctca accgggcgat gcgtccaaag ctctatgtcg gcaacaacct gttcaaccgt
     5881 attttcggga ccgggaagat cgatatccgg cctcagggaa cagtggacat catggccgga
     5941 taccagggac agaacatcca gaacccgacc cttcccgaaa gcgcgcggcg gacaggcggt
     6001 ttcgacttca acatgaatgc caacctgcag gtggtcggca acatcggcga taagctaaaa
     6061 ctcccgatca gctataatac gctcgccaat ttcgactttg agaaccagct aaagctggat
     6121 tataccggca gctccgacga gatcatcaag aagatagagg ccgggaacgt ttccttcacc
     6181 acgccgagca ccctgatgac aggtgcccag tcgctgttcg ggatcaagac caaactgcag
     6241 ttcggcaagc tgaccgttac ggcggtactt gcgaatcaac gttctcagaa acaatctgtt
     6301 aattcctccg gcggggcatc ggtcaccacc ttttcattca aggcggacga ttatgatgag
     6361 aaccggcact tcctgctggc ccagtacttt cacgacaatt acaatgcggc tatgtcgagg
     6421 atacccctgg tcacttcgca ggtacagatc ctgcgcatgg aggtctgggt gaccaaccgc
     6481 aatggtatcg acaccggctc ccggcagatc gtgggtctga tggatcttgg agagaccaaa
     6541 ccctacaatc ctaatatccg gccgctgacc aaccagcctt accccagcaa tgacaacaat
     6601 agcgaatatc gcagcatcat caacgatccc tcgagccgaa gcccgaccac ggcggcgtcc
     6661 aaaatgaatt cgttgggttt gtcgcaggtg caggatttcg agatcgtcta tgccagaaaa
     6721 ctgaacccca cggattatta tttcaatccc cagatcggtt tcatctcgct gaatcaaact
     6781 cttcaggcca acgatgtgtt ggccgtcgca tatcagtaca gctataacgg tcatatttat
     6841 caggtggggg aattctccac cgacgttccg cccgacacca cctccggcac cttcggcggg
     6901 tcatccaagg tgctgtacct caaattgctg aaggcgacct cgcagcgtac caatctgccg
     6961 atctggaatc tgatgatgaa gaatatctat tccctgaaaa cgtcggtcgg cggttacctg
     7021 agcaatatcc agcaacaagg attccagttc aatgtcaact acgatcaacc cagcctcggg
     7081 accaagcgct acctgccgga agggccgagg gcgaatcttc ccctcattac cgtgctcggt
     7141 ctggaccggt tgaatgccca caacgatccg gggcccgacg gggtcttcga ctatatcgag
     7201 gggttgacgg tcatgtccag ccagggccgg atcatcttcc cggtgctgga gcctttcggc
     7261 agggacctcg atacgcttgc ttttgcgggg cagccccaat cggtgaagaa caaatatgtt
     7321 ttctatcagc tgtacgatac gataaaagca gtggcgcaga cctatgccaa cgtggaccgc
     7381 tatgtcctgg ccggaagcgc aaagggacaa tcgacttcga gcctttccct gaatgcctat
     7441 aacgtgcctc ccggttcggt ggtcgtcacc gccggcggcc agacgcttag ggagggtgtc
     7501 gattatgtcg tcgatta
//
LOCUS       DASYSV010000353        35535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2501289, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000353 DASYSV010000000
VERSION     DASYSV010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 35535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35535
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2501289"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..306)
                     /locus_tag="VGQ51_10620"
     CDS             complement(49..306)
                     /locus_tag="VGQ51_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037069.1"
                     /translation="MKKKQPPKIKEDRPSRADFHHSSTTQGGSNYGQGSNDLDKHAEK
                     QGSEKNAGANYDNEQGWDNEALRKEDIEENQNGEKEEGAVR"
     gene            380..748
                     /locus_tag="VGQ51_10625"
     CDS             380..748
                     /locus_tag="VGQ51_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037070.1"
                     /translation="MHKLPAFILLIPVAILFIGTVRLGSICTTPAACTTPAATQCAKM
                     DMPGECGACHKHKDQPKENPGCTCCMECPLCAPVTLSPVFRLEVIRQEKTIEYAVMRD
                     NPLSDYSEQHWKPPDPSRFS"
     gene            785..964
                     /locus_tag="VGQ51_10630"
     CDS             785..964
                     /locus_tag="VGQ51_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037071.1"
                     /translation="MNILMKGACIGLLAMTTMAYAGKGKHKAPKATTQTTQTCGPNCP
                     HPCPPICTGGSCCHK"
     gene            1170..2819
                     /locus_tag="VGQ51_10635"
                     /pseudo
     CDS             1170..2819
                     /locus_tag="VGQ51_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
     gene            2997..3257
                     /locus_tag="VGQ51_10640"
     CDS             2997..3257
                     /locus_tag="VGQ51_10640"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HEV9037072.1"
                     /translation="MQTVQIYVAMAGSAIERAAKELKGFKKVFVRAGGSVDVTIDLPI
                     DDLAYYDTNAGKWKIEPGMYAILAGTSSRDINAGRGINVVAD"
     gene            complement(3326..3814)
                     /locus_tag="VGQ51_10645"
     CDS             complement(3326..3814)
                     /locus_tag="VGQ51_10645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9037073.1"
                     /translation="MQLTVLLPEHWPAVKAIYEDGLATGQASFQTSAPTWEEWDKAHL
                     THSRLIAIDSETIAGWAALTPVSGRCVYAGVAEVSVYIAARYRGQGVGKMLLSNLIEE
                     SEAGGIWTLQAGIFPENTASIRLHDALGFRQVGRRERIGQHYGVWRDTVLLERRSAKV
                     GV"
     gene            complement(3867..4598)
                     /locus_tag="VGQ51_10650"
     CDS             complement(3867..4598)
                     /locus_tag="VGQ51_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV9037074.1"
                     /translation="MKIALFSDIHANLPALEAFFASVDRQKPDAIYCLGDLVGYNVWP
                     NEVIREIRRRGIPTIAGNYDFGVGRASDDCGCAYKTEEDKANGAVSIAYTNQVIGAEE
                     RAYLRTLPAHIRVEFQLNDDKLNLLLVHGSPRRINEYLFEDREEKSMVRIMTDADADI
                     LCFGHTHKPYHRVFDKRHAINIGSVGKPKDGDPRGCYVILTVDEHSGIGVEFIRSTYD
                     VEKAARAVENSPLPRTFAEGLRKAQ"
     gene            complement(4612..5457)
                     /locus_tag="VGQ51_10655"
     CDS             complement(4612..5457)
                     /locus_tag="VGQ51_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEV9037075.1"
                     /translation="MQTTEQLKELVRRKYGEIALQDRDTNQSSCCGSGCCSDEVYNIM
                     TDDYSNVEGYTPDADLGLGCGLPTQFAQIEKGDVVIDLGSGAGNDCFIARAATGDSGK
                     VIGIDFTPAMIEKARANAAQLGFNNVEFREGDIEKMPVSAGVADVIVSNCVLNLVPDK
                     EAVFREMYRVLKPGGHFSISDIVLVGALPQKIREAAEMYAGCVSGAIGKEAYLGLIDA
                     SKFTRVLIQKEKAIHLPDDILAQYLDAAGIEEFRGSGTGIYSITVYGEKPLTTQPCCA
                     PGCCS"
     gene            complement(5508..5840)
                     /locus_tag="VGQ51_10660"
     CDS             complement(5508..5840)
                     /locus_tag="VGQ51_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV9037076.1"
                     /translation="MGVTKSEIFTDKQNKLASMMKALAHPARIAIIQHLLKTNACICG
                     DLVDELGLAQPTISQHLKELKTAGLIQGTIEGTSVCYCIEPKMWALFQKDIGALFAAY
                     KVDGNCAC"
     gene            complement(5969..6364)
                     /locus_tag="VGQ51_10665"
     CDS             complement(5969..6364)
                     /locus_tag="VGQ51_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9037077.1"
                     /translation="MHANSHSTDPAGSRCKQVLLIEDEGEMCLLLNLILDDKELCINH
                     VKSLADAAIFLKKNKPDLVLMDNRLPDGFGFDFIGYVKAHCPETKIIMISGVDKAAGD
                     FALEIGADLFLPKPFTKAALLQSVSKLLN"
     gene            6530..7225
                     /locus_tag="VGQ51_10670"
     CDS             6530..7225
                     /locus_tag="VGQ51_10670"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV9037078.1"
                     /translation="MKKSFTGRRDFIRTAAAFTGGFAGLSLLPSATMAGQQSGDGFVP
                     PPSGPAADDINIIGPKEGYSPHIGTLVSMMAWLRPQVIGSVKDLTTEQLDFLIDDKAN
                     TIGALLNHLAATDAFYHEHTFTGVEWGKWDPAVTEKFGAAMELGDEGRKKIKGHDVKY
                     YLDLLWQTRASTLTEFKKHDDKWLMTVDDKWPWGPTNNYCKWFHVCEHESHHLGQINL
                     LKSRVPGVKSSNM"
     gene            complement(7410..9773)
                     /locus_tag="VGQ51_10675"
     CDS             complement(7410..9773)
                     /locus_tag="VGQ51_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEV9037079.1"
                     /translation="MRRLPFFLLTLSVILYSSHPSNAQPTPPRLGHDPVSDIVKQMTL
                     EEKVKVVVGKGFSVPGMRMRANDSTPDKLAAISGHTIPVSRFGIPSVGMADGPAGIHR
                     FMMTAQDSAEGLFSTAWPVGTLLASSWDTALVKKVGVAFGEEIKSYGIDFILGPGANI
                     HRNPLGGRNFEYYSEDPLVSGLTAAAIINGIQSQGVGATIKHFAANNQETDRNSVNEI
                     ISERALREIYLRNFEIAIIASHPWAVMSSYNLINGVYTSESPDLLTTVLRKDWGYKGF
                     VMSDWFGGKDPVAQMKAGNDLLMPGNPDQVKTILEAVKSGKLPVADLDRNVEHILNVL
                     VHTPTVNRYAYSNKPDLKAHAQISREAAAESMVLLKNGNQCLPISNGSATIALFGNHG
                     YDLIAGGTGSGDVNKSYAVSLAEGLTNSGYRVDEGLARRYKDYLADYAAKHPKKPLIQ
                     EIMNPTPYAPEYVFDAGSIGPRADAADLAIVYIGRNAGEGNDRKIADDYELTGPEKDM
                     IAAVSTAFHAKNKKVIAVLNIGGVIDVTGFRDEVDAILLAWQPGQEGGNAIADILSGK
                     VNPSGRLATTFPVRYTDVPSAKNFPGKEFPEKATTSVVGRSVPAEVTYEEGIYVGYRY
                     FNTFNVKPAYAFGYGLSYTDYKYGDLRLSSADFSGRLRATVTVTNTGRVAGKEVVQLY
                     LSAPHHSMDKPAEELKAFGKTRLLQPGESQTLEFTLLPRDLASFSTGQSAWIAEAGEY
                     TVKIGASSEDIRLSKTFRLAESVTAGKVNRALAPKAKVEELKPVAGR"
     gene            9858..10631
                     /locus_tag="VGQ51_10680"
     CDS             9858..10631
                     /locus_tag="VGQ51_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV9037080.1"
                     /translation="MADTLIDDIFTHERDYLHNVSIDNVIFGYHGKELKVLLQQPFGA
                     GKWTVTGGYIRRTESIEAAAARIATARTGLKDLFLQQFRAFGNPRRTAENGFTAAQLS
                     KLTGTEVRPNAWIFDYFVSVAFYTLTEFSKVQVTKSPLEADCAWWPVGDLPPMLFDHD
                     LIIKEALNAMRIHIAHFPIGYQLLETKFTMPEIRSLYETILGRSLDDRNFSKKLMATG
                     ILTKLEETRKIGAHRSPYLYRFNKTKYEVALKTGIELAF"
     gene            complement(10628..11479)
                     /locus_tag="VGQ51_10685"
     CDS             complement(10628..11479)
                     /locus_tag="VGQ51_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9037081.1"
                     /translation="MKTIQLEKIDFETGKSFKVFSPRLRNTFLWHYHPEFELVYVEAD
                     AGIRHVGRHISTYTQNDLVFIGGNLPHLNFDYRLRSEYHQIVIQLRTDFLGTAIGVAP
                     ELGGIAKLFHEAAYGIAFSGKTKAEVAGLIKEMSDMTPLDQLLRLIGVLQRLADSREW
                     VRLNDDLDSQSFILKDKIRMGSVYEYIAAHYDRGPDVNVVARKVNLTTPAFCRYFRRQ
                     TNMTFTAFVNQYRVERAKNLLMQNHKVGETCYAVGLDSLSYFNKLFKELTGENPSDFR
                     ARHIGGS"
     gene            11584..12555
                     /locus_tag="VGQ51_10690"
     CDS             11584..12555
                     /locus_tag="VGQ51_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEV9037082.1"
                     /translation="MNMTPAAGAMTPTLDAHKEIPGNPSTAKSSRLRLNDRSNGEPLR
                     VLTEEDWRFWITNGYIVIKHAVPREQALRLADYLWKYEDKDPNDKTTWYRKPNVDMQM
                     KELVNTGMVEIYNHQYLWDNRQYPTVHQAFADIWGTERLWVTIDRANLNFPQAPGFEY
                     KGFIHWDYDPETRPQNVQGVLALADQTDENMGGFQCIPDLFRTYDTWKLTQPEDRDHY
                     KPDTTGFDIVKVPLEAGDLLIFNSLQPHGIRPNRSGDKVRIAQYISMMPAQEENDELR
                     QWRINSWQSRDPMEGYAFPGDPLQREKQNPVATLSPLGERLLGLTRW"
     gene            complement(12602..13000)
                     /locus_tag="VGQ51_10695"
     CDS             complement(12602..13000)
                     /locus_tag="VGQ51_10695"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV9037083.1"
                     /translation="MKISAKLTSTHHRQDVVVQTNDSAKSLALPTKPSGFGSAINGGE
                     LLLLALATCYCNDIYREAAKKNIPVTGVEVECTGTFGAEGEPGDNFCYNVKISSDAAA
                     ETIEKLIRATDGVAEVHNTLRKGVSVKLTR"
     gene            13077..13553
                     /locus_tag="VGQ51_10700"
     CDS             13077..13553
                     /locus_tag="VGQ51_10700"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9037084.1"
                     /translation="MQKISTFIWFDNNAEDAMNFYVSIFKDSKVIRVARNPPGAPGPE
                     GALLVASIQLEGQEFILMNGGPGHPLTDAISLTINCVSQEEVDHYWDRLLAGGGTPIA
                     CGWLKDKFGLHWQVTPTILPQLIGDPDKAKAGRVMQAMMKMVKLDIPTLKAAADAK"
     gene            13672..14907
                     /locus_tag="VGQ51_10705"
     CDS             13672..14907
                     /locus_tag="VGQ51_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEV9037085.1"
                     /translation="MTSFQSAKRLLLLLLAGFSGLTAAAQYRNVFLEAGYRRSDIDSK
                     VDKVYRDLFEGPNKVYFEVGDSMGYVSDLKNHDARTEGLSYGMMIAVQLNKKDVFDRI
                     WRWSKRYLQHADGPRKGYFAWSIDPATLHKNSEGTASDGELYFVTDLLFASNRWGNRT
                     GIDYYHEARTILDDMWKKNGSGGIYPFINTAHRLMTFTPEGDNYNWTDPSYNLPAFFE
                     VWAMYAKDGHEQFYKECADSARAFLHRACDPVTGLNADYTEFSGAPHSTRWMPPGFRY
                     DSWRVPMNIAMDYNWFGKDTAWQRDYARRFQSFLLSKGLDSFPDQFNTDGSAPAAILQ
                     AGGFKKLRHSLGLLATAASTTLIYNRDHSFEFARALWNAKLQPYADGYFDPYYDGLLY
                     LFALMHLSGKYGIIRPQGR"
     gene            14916..15680
                     /locus_tag="VGQ51_10710"
     CDS             14916..15680
                     /locus_tag="VGQ51_10710"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV9037086.1"
                     /translation="MEYETYIPSDPLKPYIRSFAIQETAAEATYRVLPDTGIVVGFQY
                     KGRLFRIDEGTPSALDTSGLSGLADHGRTFRNLSGTGTILVFFKEAGAAAFFRQPLHE
                     VFRESVSLDNFLLRSELLCLEEQLIEAPSNPERIARLEKFFIGRMSRTEPDKLVLAAL
                     ALIHQTRGNIRIRDLAVQLHTSQSPLEKRFRAAVGASPKKFASIVRLKNVLQQYSATN
                     SLTELGYEAGFYDQAHFIKEFKALTGDTPEDFFKKE"
     gene            15836..16504
                     /locus_tag="VGQ51_10715"
     CDS             15836..16504
                     /locus_tag="VGQ51_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEV9037087.1"
                     /translation="MRRMKTYDVPHKGLRNGLSQLALMAGRTDYSNPMEVTQLHQLGA
                     RLFSILTIHAEDENAVTLAHLETRCPGSTRHDLDDHEEIHRTQQQLEKQLDAIHAGAR
                     DGKDMTEAGAEFYLAFTEFQSEYLGHTAEEERVTQPLLWQYFTDEELAAHRGQIMARN
                     PPRTLLTWFEFVFPAQSHAERVGLLTGFRKMAPAGFFAEAMEVIRKALSPSEFGRLED
                     ALAI"
     gene            16741..17124
                     /locus_tag="VGQ51_10720"
     CDS             16741..17124
                     /locus_tag="VGQ51_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9037088.1"
                     /translation="MKRVIGLGGIFFKCNDPKKVTEWYQTHLGLQSSPYGVKFDWRQD
                     GDSTKKGFTLWTPFPDKTKYFEPSSRDFMINYRVENLETLVEELKKEGVTVLDKIETY
                     DYGKFVHILDPEGNKVELWEAIDKQ"
     gene            complement(17243..19624)
                     /locus_tag="VGQ51_10725"
     CDS             complement(17243..19624)
                     /locus_tag="VGQ51_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037089.1"
                     /translation="MFKHYMLLTWRNLTRNKLYSAINIIGLGTGMAACVVILLFVFYE
                     KSFDSMHHKNLYRLNEVLKFTATGNSEKSAITGISTGPTLKDEFPEILNYSRVDLNSQ
                     YEMTYGEKRVFFPYTYFVDTSFLQLLDFPLVEGDRQTALQKPNSIVLTQTAARTLFGT
                     VDPIGKTVSHFDTDTVLYTVTGILKDVPANSQFQFDALQSFNTVLRTRSHDGVSTYLE
                     LAPHTDIAALEKKFPTYLKKYKIGDQHIRHDLFLLPLRDVHANAVDIGDDDINFQKFD
                     RRYTDIFMGIGLLVLLIACINFMNLATAQSAGRAKEVGIRKTIGARRPQLAVRFLIDS
                     VVLSLISLVLALAIVSLVLPYIDDLSGRQLSPLFYLHPGLLAGVFIGTILLGILSGLY
                     PAIYLSSFSPVKVLKGGSESGRSKGILRNILVVTQFAGAIFLVIVTILIFRQLMYMEK
                     QDPGFDRDQVITMRLHGITDRKYALLKEELSVSPLITGVTGAQDHLGGPLATLGFGFW
                     PGDAPMRVLFTPGIFVDPDYLTVYKISLEAGRNFTGDTSGYGNEFIINETMARDLLHD
                     RPGTPLSWLIGKHFGDTLGRIVGISKDFNFNSLHYKIEPMFLLDQGASSFNTVSVRIN
                     GRKAEQAIAFIESTWKKILPEYPFSYQFLDDHFRQLYRTDTQISQMVAIMAGLAILIS
                     CMGLLGLASFSTERRTKEIGIRKVLGASVKDVLVLLTRHFIGLVLIANLIAWPLAWFA
                     LHRWIQDYAYRVSISWWVFVVAGVAALVIALGTVSFLALRAARNNPVRSLRTE"
     gene            complement(19797..20255)
                     /locus_tag="VGQ51_10730"
     CDS             complement(19797..20255)
                     /locus_tag="VGQ51_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037090.1"
                     /translation="MKSIILVLGALAIAQIAPAQEHRNQQQVPSNVRNSFQRDYPKAG
                     DTRWSESGGQWHADFTDRSPKDRGEMTAHYDRHGRHIDSHIPYDDRDVPSEVRRRTEH
                     RYPHGRDYSYTRIEHPGGQPLFQVSLNLGGAHKTTYVDDQGRPRTYHDGH"
     gene            complement(20365..20742)
                     /locus_tag="VGQ51_10735"
     CDS             complement(20365..20742)
                     /locus_tag="VGQ51_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037091.1"
                     /translation="MLILIAGPYRSGTGDDPGLMRKNLDYLESVALPLFRKGHMPMIG
                     EWAALPLIKLAGSVRPGDAAWDTIQYPTAHRLLEKCDAVFRLEGSSKGADNDVKLARE
                     RGLPVYYSLEDVPEANMPPSGNI"
     gene            complement(20797..21372)
                     /locus_tag="VGQ51_10740"
     CDS             complement(20797..21372)
                     /locus_tag="VGQ51_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV9037092.1"
                     /translation="MEHEPILDNIAKHITLDRGETEYFLSLTTSSELPKKTLLLKEGQ
                     VCNQLSYVSSGALRAYYVDKTGKESTILFAVADWWITDMYCYLNHRPAMMYIETIEKS
                     CIVQISKTNHDELYKKVPKFDRFFRILMQNAYTREQLRVIENLSLSAKEKYDSFLRKY
                     PQVAKVVTLKQMASYLGITPEFLSTVRGMKS"
     gene            complement(21451..22110)
                     /locus_tag="VGQ51_10745"
     CDS             complement(21451..22110)
                     /locus_tag="VGQ51_10745"
                     /inference="COORDINATES: protein motif:HMM:NF020498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEV9037093.1"
                     /translation="MPTQTLPGSVKPAPAGAMGFDVNHVLTADQALDFKNAGYNFCIR
                     YVPRTAALAPGNITNAEALSILAAGLALMPVQHVSLPGWKPTTNLGTMYGNFAATWCA
                     QTVKLPKGMNVWCDLEEVADGSPAQDVVSYCQAWYNAVHAAGYVPGLYVGYGTGLTPD
                     QLYNDTSFQHYWRAYNGGQVTTRGFQLLQKTELTLHGITFDPDVTQLDHEGDAALWLS
                     A"
     gene            22202..22960
                     /locus_tag="VGQ51_10750"
     CDS             22202..22960
                     /locus_tag="VGQ51_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV9037094.1"
                     /translation="MKKLLALLACVMAAGLAQAQPSRFSRERYIENGDTLSYRLLFPD
                     ANRQRKYPLVIFLHGSGERGSDNDAQLKWGVMNFATDRNMMEHPALVIAPQCPEHQTW
                     SSFTPDKNKMGMTLNAEPGKPMQLLIGLIRQLEKTMPVDTNRIYITGLSMGGYGTFDA
                     LERYPHLFAAAVPVCGAGDTSRAASIAHIPMWIYLGAEDPAVNPLYGLAMFEALTNAG
                     AHPGFTQYPEVGHFSWLGAYSDPLMMEWLFRQHK"
     gene            complement(22967..25402)
                     /locus_tag="VGQ51_10755"
     CDS             complement(22967..25402)
                     /locus_tag="VGQ51_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037095.1"
                     /translation="MIRNHFITAWRNLWKNRISSFINIVGLTIGLTSFILIALYVFDE
                     FTFDRSHDDAANIYRVVENRTSPDGKTAKRAGTGSQVSARATTSFPEIQDAARISVYW
                     RASVASGDNRSRVFQEDFIAGNPGFLHVFSFPLIYGDRSSALTDPRSIVLTETTAKKF
                     FGKSDVVGRSLFFDNDSVPYRVTGVLKNFPANSSISFDLLVSEVSLPKDWLANDWTSG
                     AFATYFLLKPNTDIPALNKKLDNLIAANHNAEAGVKSLVQLQALTDVHFYSNDIEGNS
                     GKRGNIYYIYVFLIVACFIIFIACINYMNLATARFTNRGKEIAIRKVSGASRATLIKQ
                     FLAEALMATLFSVLLSLVLVNLLLPLFNSFTEKHLTLNAHTDYRIWTGVLSILAIVTL
                     LSGLYPALFQSGLNPLALLKSKIQLGRGNISLRRSLVVFQFMISIVLIAATIIIYQQM
                     QYVNKKDLGFDKDKLVVIDNDGGIFGRRAGTIKNEFSKLARVSSVTATSMVPGESKTI
                     PSVKVNMNGENSATGKDIYFFGVDGQFLSTYDIPLIKGRNFFPTGSGDSSSVLINETA
                     AKELGITDARNQVITISSAKTGGDTRSAIEKPLSLTIAGIVKDFNFQSLHEPVAPMML
                     GFKRDVTQNYGHFTVRLVGGDPSGTLKSMKAIMQRMDQGGLFQYHFLDKQWESLYRED
                     RTRQTIFIIVSLLAIFIATLGLLGLTIYAAEQRVKEIGIRKVLGANIGGIILMLSKEF
                     LKLVLVAALIAIPIAWFFMHKWLQDFAYRINIDWWVFVLSALVAVMIAMATISLQVVK
                     AAMANPVKSLRAE"
     gene            complement(25538..26224)
                     /locus_tag="VGQ51_10760"
     CDS             complement(25538..26224)
                     /locus_tag="VGQ51_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV9037096.1"
                     /translation="MRKIIVTEFITLDGVVEAPGGNETPHPHGGWQTKFGHPEGGKYK
                     MDELAAVDALLLGRTTYEVFAGYWPGQGGNGFGDRMNQLPKYVVSRSLKKVDWNNTHI
                     LRGVKGGDVAGRGAAESGMTGRDVKGGDVAARGVAGGGVAGDADAGDVASLKKTDGGD
                     ILVYGSATLVKALLHHDLIDELHLMVYPVCVGGGLRLFEDNRELKKFGLKQSRALDNG
                     VIILEYQLTS"
     gene            complement(26236..26730)
                     /locus_tag="VGQ51_10765"
     CDS             complement(26236..26730)
                     /locus_tag="VGQ51_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV9037097.1"
                     /translation="MNSTLQFDFSVDRTTNSIFVNRDFAAGLSKVWDAFTKQEILDQW
                     WAPKPWISRTKHMDFTAGGRRFYAMVSPEGQEFWSLQKYTSVSPTTNFKYQDAFADKD
                     ENINEAMPRSDWDLSFSERNGTTSVSIVIKLKTLADLEQLIQMGFKEGFTMTLNYLDQ
                     LLKP"
     gene            complement(26727..27047)
                     /locus_tag="VGQ51_10770"
     CDS             complement(26727..27047)
                     /locus_tag="VGQ51_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV9037098.1"
                     /translation="MRRDIFQAIADPTRRAIIALIALQAMTPNAIAENFNTTRQAVSK
                     HLRILTECDLVKQEYQGREIYYSLEIEKMKEIDKWLSQFRKIWETRFDQLDKVLSTIK
                     KQKK"
     gene            27196..27894
                     /gene="msrA"
                     /locus_tag="VGQ51_10775"
     CDS             27196..27894
                     /gene="msrA"
                     /locus_tag="VGQ51_10775"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEV9037099.1"
                     /translation="MKKLLCYILLSIIAVCPAFAVPAGPNLPNSPDGPNVPNSPGGPD
                     LPNGPGAPKLDTATFATGCFWCTQATFQQLRGVTRVTAGFTGGHTVKPSYEEVCTGTT
                     GHAEACNIVFDPRVISFDTLLEVFFTVHDPTQLNRQGNDVGTQYRSAVFYHNVVQKQK
                     AEYYIRKINEAKAYPRPVVTRVVPFTVFYKAEDYHQDYYNRNGNVPYCKYVIQPEVEK
                     FRKIFADKLKTTHE"
     gene            27887..28411
                     /gene="msrB"
                     /locus_tag="VGQ51_10780"
     CDS             27887..28411
                     /gene="msrB"
                     /locus_tag="VGQ51_10780"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV9037100.1"
                     /translation="MNKIMRTFIIAAVLALGVAARAFPQHLKSPEGHPNNPYYSNTDT
                     NKLHVSDAEWKKILSPALYATAREQATEKPFTGRYWDQNAKGTYYCAVCGNKLFRSDA
                     KFSSTCGWPSFFEPSRKNSVTYLQDHSYGMDRIEVRCARCGSHLGHIFDDGPPPTHKR
                     YCMNSISLDFKPDK"
     gene            complement(28540..28610)
                     /locus_tag="VGQ51_10785"
     tRNA            complement(28540..28610)
                     /locus_tag="VGQ51_10785"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28576..28578),aa:Cys,seq:gca)
     gene            complement(28756..28926)
                     /locus_tag="VGQ51_10790"
     CDS             complement(28756..28926)
                     /locus_tag="VGQ51_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037101.1"
                     /translation="MTRSIADKIRSLTEKARSLGPQKVKRGTAVRNGSSLHKIIRTKE
                     QADKLMKQLQGN"
     gene            complement(28965..29531)
                     /locus_tag="VGQ51_10795"
     CDS             complement(28965..29531)
                     /locus_tag="VGQ51_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037102.1"
                     /translation="MALTVNIDFDHIYEIEPVTPDLRTTTFKSQLADGTSLPLRVEIS
                     NQPHPLLPDVYNLAFGPLNSRGLVDDKAEIEHSDYSKTFSTILFTALTYLSSNPSHFL
                     GIDGSNNARAYLYYRFIQRNFGYLAQFFIMHGLKYYVRISRFGKNQFDNPFDFKDIYT
                     SLDPISRGRIIRSDWMYNYFIFRLNTAR"
     gene            29654..31135
                     /locus_tag="VGQ51_10800"
     CDS             29654..31135
                     /locus_tag="VGQ51_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV9037103.1"
                     /translation="MKPLLAAPVHITAALLVLLYPTGLCNAQQPAPAAKPGTTTVTAP
                     PAIATAQPGATTVTEWQYGRNGALSITYDDASRHQFTKALPLMERLKLPATFFVITGP
                     ITGSKYHGRFIGRPVQEIIRESATIPTNDNNFFERCSAAGYLGYQGTIAYHTRAGGMY
                     SSGRKQQAYHLMDSLYTKVRHGDFPQGYEPCPEYKEAEGSSWNDFRKDVAEGYEIASH
                     SITHATMPGMDEANIKYELEKSKEEIRNQLGDKYTFSTEVPYGYEDDRVMQIAYKIYP
                     ALRNRMPEPFLKEIDRGSRMLPHPNDKDYVQWQRGPLHATPLPLMESWVDTAAATHDT
                     WLVLVFHGIDSLGWEWTPMPKLEEYFNYIKTREDKLWVATFGDVTKYMREREAAKVTA
                     NRTSETAAAPKTETPAKSSNPASSNPANSGTIIITLTHSLDPKTYTLPLTLKTYIPDT
                     WTKPTVIITQGTTRQHAAVQKDDKGKYILYQAKPNTVNVTLHP"
     gene            31132..32400
                     /locus_tag="VGQ51_10805"
     CDS             31132..32400
                     /locus_tag="VGQ51_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEV9037104.1"
                     /translation="MKRDYYIVGLIMLTFFVISFLTNVIQALNPDFITGFHLSLTLAA
                     VLPFAFFIAYGLVSIPTGMLLEKYKEKKIMVAGFVVAVIGSVLLAVIHNYLTAIISLF
                     LIGAGMAMLQVVINPLLRTAGGEEHYAFNSVLAQLIFGAASFLSPLVYSYFAQHLNGQ
                     TSGLMGLFNSIVPGNLPWISLYWIFAVISVAMIVVIKTSRFPVVELKADETAGPWKTH
                     LMLFKKKVVILYFIAMICYVGTEQGIGNWISKFLETYHHYDPKTKGADTVAYFWGLMT
                     AGGVLGLFLLKIMDSRKLLIAFTIAALVCLSAALFGSGPVALIAFPAVGFFASVMYPI
                     IFSLALNSVSEHHGSFAGIMVTGIIGGAIGPLIIGGLGDALGLRTGMFFLYLTLGYIL
                     SIGFWARPLITNRTVFNKNDFYETAQSDRH"
     gene            32372..33220
                     /locus_tag="VGQ51_10810"
     CDS             32372..33220
                     /locus_tag="VGQ51_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV9037105.1"
                     /translation="MRQPKVIGIDLGATNIRGAVVSGDSLSDIISVRIHSEGSVEDVL
                     NDIYQLIDRLTGPDIAGIGIGVPSVVDVEEGIVYDVQYIPSWVEVPLKQLLGERYRLP
                     VYVNNDANCFALGEYYFGKGKGMSSLVGLTIGTGLGGGVVIHDKLYPGYNCGAGELGL
                     LPYRDNILEYYCSGSFFKNLHGLDGVMVYEAAREGDARALELYAELGEHIGQGIKAVV
                     YSYDPQLIVLGGSVSAAYDLFQQTMWETIRSLAYAKTLARLKIEVSNLKNSGILGAAA
                     LYYDST"
     gene            33207..>35535
                     /locus_tag="VGQ51_10815"
     CDS             33207..>35535
                     /locus_tag="VGQ51_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037106.1"
                     /translation="MILHNHYTQIIAVLAFSFAMTPMTPASAQSPGHYSMVLKEGWEM
                     QSVTKVPDDGATISQKKYTPEGWYKVEVPTTIIAGLLANKVYDFDPFYGMNLEKLKDS
                     SLNRPWWFRKEFTLPATQKGKDVILTLHGINYKANVWLNGVLVADSGRIMNPYRIIAL
                     NVTPYIKAGGANVLALEIRRPINPQRRGGDLAIDYADWIHYPPDYNGGIVNDIVIKTF
                     DHVGIEYPLVTTHFDLPSLDVAHLTVDAMVGNYSSGPQDVVVKGRIDSSITFQKKVHL
                     EANEFADVTFTPADFPQLNVKHPRIWWPWQYGKAELNRIELSVMRGGTVSNSLSEDFG
                     IREITSLFLDTAARVFIVNGKRILIRGAAWSPDIFQRRSPRRQEQEIRLYRDMNMNLI
                     RSEGKLEDDNFYALCDKYGMLVMTGWMCCGAWQYPELWDAAKRASAMASDSSVMLWLR
                     NKASVMVWLNGSDMPPRDTSVEREYLAIESALKWPNPTISTADASKSKVSGYSGVKMD
                     GPYEWVPPIYWETDSTRRHGGAWSYATEISPGPSIPPYESLIKFIPKDSLWFTTATWK
                     YHCGTMNFGTTDVFNTALQARYGEAKDIRDYVDKAQAQNYEAHRAMMEAYGLDKYHDA
                     TGVVQWMGSNPWPGLIWHTYDYYLYPAGTYFGMKKALEPLHIQYSYARNAIAVTNSLL
                     HRFTNLRADAKIYDLDGSVKWSKERQLSVEPDSVWLGLDVPAIAGLSPTYFLRLELKD
                     EIGKVKDINWYWLSARGDELDWKTSKWFVTPEKAYA"
BASE COUNT         8325 a         9881 c         9234 g         8095 t
ORIGIN      
        1 ttgtccgtac ttcgccataa aataatgtat atagatgcgc ggccgcaatt atcgtaccgc
       61 accttcctct ttctcaccgt tttgattctc ctcgatgtct tcttttctaa gcgcctcatt
      121 gtcccatccc tgttcgttgt cgtaattggc gcccgcgttt ttctcgctgc cttgtttctc
      181 cgcatgtttg tcgagatcgt tcgacccctg tccataattc gacccgcctt gcgttgtcga
      241 gctatggtga aaatctgcgc ggctgggccg atcttccttg attttgggcg gctgtttttt
      301 cttcatacga ttcgcttttt ggtatgcgat gcaaaagttt cgccaaccac tgccgattta
      361 gtgttatctt gtgggaagca tgcataaact cccggctttc atattgctga tcccggtggc
      421 catccttttc atcggtacgg tgagattagg gtctatctgt acgacccccg ccgcctgtac
      481 gacccccgcc gcgacccaat gcgctaagat ggacatgccg ggggaatgcg gggcctgtca
      541 taaacacaaa gatcagccaa aagaaaaccc ggggtgcact tgctgtatgg agtgcccgtt
      601 atgtgcgccg gtgacattga gtcccgtgtt ccggctggag gtgatccgcc aggaaaaaac
      661 gatagaatat gcggttatgc gcgataatcc tttaagcgat tattccgagc aacattggaa
      721 accacccgat ccttcccgat tctcctagtg tcctcaacat tttttcactt ttaatctttt
      781 caccatgaat atattgatga aaggggcatg cattggcctg ctggcgatga ccacgatggc
      841 ctatgcagga aagggcaaac acaaagcgcc gaaagcgacc acgcagacga cgcagacctg
      901 tggtccgaat tgcccgcacc cctgtcctcc gatctgcacc ggaggttcct gctgtcataa
      961 atagaaaaac gattatattt atataaccgc attaacaggt ccccgcgtgt cggggacctg
     1021 ttttttaacg ctgcaagatg aaaaaactgt taaccatcct actggcaggc ctcgccggtt
     1081 gtgccttccc gcccggtgcc tcggcacaga tctccgatga ctccatatcg gctattgtca
     1141 aaaagctgac cctcgaagaa aaggtggcca tgcttcatgc gaatagtttg ttttcctcgg
     1201 ccggtgtcag acggctggga attccggacc tcgtttgcga cgacggtccc ctaggtgtgc
     1261 gcgaagatgt actggcccat tggcagcccg ccaatctgcc cactgattct gccaccttct
     1321 ttccgaatgg ctcagccatt gcggccacct gggatcccga cctggcctat cggtacgggc
     1381 acgacctcgg tcaggaagca agagcccggg gcaaacagat catcctggcc cccgccttca
     1441 atattacccg caccccactc aacggccgca cctatgaata ctactccgaa gatcctttcc
     1501 tcaacgcccg tttgaccgtg gcctcggtac gcggcattca aagccagggc gtggccgcct
     1561 gcatcaagca tttcgcggta aacaaccagg aacaggaccg cggccggatc gacgtccttg
     1621 tcgatgagcg cgcactgagg gaaatttatc ttcccgcctt taaggcagcg gtcaccgagg
     1681 ccgatgcatg gaccatcatg tcggcctaca ataaattgag aggagtctat tgttcggaga
     1741 atgaatacct cctcgatacg atcctgcgga aagagtgggg tttcaagggt gtggtcgtca
     1801 gcgactgggg cggcacacac agtaccgtcg atgcggcaaa tcatggactc gatctcgaaa
     1861 tgggctcggg tgagatatat aacaaatact tttttgccga tagcctgctg gccgccgtaa
     1921 aaacgggccg ggtcagcgaa gccgtgatcg atgacaaggt cgcacgcatt ctccggctga
     1981 tgtttaagac ctcgctgaac cctaacgcgg gcgccggcag catcaataca cccggtcaca
     2041 ttcagacggc ctatgatatc gcggccgaat ccatcgtcct gctaaaaaac gataaccagc
     2101 tgctgccgct gaaaccggat agggtaaaaa acgtggcggt catcggcgat aatgcggttc
     2161 atacttttca taacggcggg tttggcgccg gagtaaaggc gcggtatgaa gtgacggcac
     2221 tcgaaggctt gaagaacagg cttccggccg gcgtgagtat acgattcgcg cagggctaaa
     2281 ctgcactgcc tcaccgccgc gggacgacag caaaagccga ttcgggcgcc atcgcagcga
     2341 agttgatctc cgaagctgcc accctggcga gacaatccga tgtcgccttg ttgttcgtcg
     2401 gaggcaaccg cgactatgaa agtgaatcgt gggaccgaac ggacctctcg ctcccttttg
     2461 gcgagatggc tcttgtcgac gcggtcacgg cagccaatcc caggacgatc atcgttgtcg
     2521 tgggcggcgc tccctacgat ctcaacgcca tacagaaaaa acatccggcg atcctgtggt
     2581 catggtataa cggttctgag aacgggaatg cgttggccga tgtgctgatc ggtaaggtca
     2641 acccttcggg caaattgcca ttcaccttcc ccgtcgccct ctcggatggc ccggcgaatg
     2701 cgggtggggc ggacacgtat cccggcgttc atttgcagga gacctataag gaagggatat
     2761 tggtcgggta ccgctggtat gatacgaaga agatcaaacc tctttatccg tttgggtacg
     2821 ggctttcgta tagcggttgg tcgttctcga atatgaacat tagtcggctc cccgcgtcct
     2881 ccacggcgct tatccgggag aagggaggtg aaatacaggg gacgaggcct atatttaaaa
     2941 aaggagatac ggcgaccgtc cggctgacgg ttcacaatac gggccggtac gacggcatgc
     3001 aaacggtaca gatatatgtt gctatggcgg gttccgccat cgagcgcgcc gcaaaagaat
     3061 tgaaagggtt caaaaaagtg tttgtccggg ctggtggatc ggtcgatgtg accatcgacc
     3121 tacctatcga cgacctggcg tattacgata cgaacgccgg caaatggaag atcgagcccg
     3181 gtatgtatgc gatcctggca ggtacatctt cgagggacat caatgcagga cggggcatta
     3241 atgttgttgc cgattgaacc ggaacccggg ccggaagcgg tgccctcctc cggcagggaa
     3301 aagcatatat gccctgcgcc aaaacctata ccccgacctt cgcgctcctg cgttcgagca
     3361 gcaccgtgtc ccgccatacc ccgtaatgtt gaccgatcct ttcccgccga ccaacctggc
     3421 ggaagccgag ggcgtcatgc agccggatgc tggcagtgtt ctcgggaaag atgccggcct
     3481 gtaaggtcca gatgccgccc gcctcgcttt cttcgatcag gttgctcaac aacatttttc
     3541 ccacaccctg accacgataa cgcgcagcga tatagacgct gacctcggcg acacccgcat
     3601 aaacgcaacg accggatacc ggcgtgagcg ccgcccagcc ggcgatggtt tcgctgtcaa
     3661 tggctatgag gcggctatga gtcaggtgcg ccttatccca ttcctcccag gtaggcgcgc
     3721 tggtctggaa actggcctga ccggtagcaa ggccgtcctc atagatagct tttacggcgg
     3781 gccagtgttc ggggagtagg acggtaagtt gcatcttcgc gcggattttt agcgttagtt
     3841 ggcgatctgc cagcatacgt ttctgtttat tgggcctttc gcagcccttc agcgaatgta
     3901 cgcggaagag ggctattttc gactgcccgg gcggcctttt ccacatcgta ggtcgatctg
     3961 atgaattcga caccgatgcc ggaatgttcg tcgactgtaa ggatcacata gcagccgcgc
     4021 ggatcgccgt cttttggttt gccgacggag ccgatattga tggcgtgacg cttatcgaag
     4081 acgcgatggt acggtttatg ggtgtggccg aaacacagga tatccgcgtc ggcatcggtc
     4141 atgatgcgca ccatactctt ttcttcccgg tcttcgaaca ggtattcgtt gatgcggcgg
     4201 gggctgccgt ggacgagcag gagattcagt ttgtcgtcgt tcaactggaa ttcgacccgg
     4261 atatgcgcag gcagcgtgcg gagatacgca cgctcttccg ccccgatcac ctggttggta
     4321 tacgcgatcg aaacagcccc attggccttg tcttcttcgg tcttatacgc gcagccgcaa
     4381 tcatcgctcg cccgccctac accaaaatca tagttaccgg caatggtagg aataccacgg
     4441 cggcggatct cacggatcac ttcgttgggc cagacattgt aaccgacgag atcgccaaga
     4501 caatagatgg cgtcgggttt ttgccgatcg acgctggcaa agaaggcttc gagagcaggg
     4561 aggttggcat gtatgtcgga aaagagtgcg attttcattt ttcgggacat tttaggagca
     4621 gcaacccggc gcgcagcagg gttgcgtcgt caaaggcttc tcgccgtaca cggtgatgct
     4681 gtatatgccg gtgccggacc cgcggaactc ttcgatcccg gccgcatcca ggtattgcgc
     4741 aagaatgtcg tccggcagat ggatggcttt ttccttctgg atgagcaccc tggtaaattt
     4801 actggcatcg atgagcccga gataggcctc cttcccgatc gcaccggaga cgcatcccgc
     4861 atacatttcc gctgcctccc gtatcttctg cggcagcgcc cccaccagca cgatatccga
     4921 tatgctgaaa tgcccgccgg gtttcaaaac ccggtacatc tcccggaata ccgcctcctt
     4981 atcgggcact aagttcagca cacagttgga gacgatgacg tccgcgacac ccgcgctaac
     5041 gggcattttt tcgatgtcac cctcgcggaa ctccacattg ttgaacccca gctgcgcggc
     5101 attggcgcga gccttttcga tcatcgcggg ggtaaagtcg ataccgatga ccttaccgct
     5161 gtcgccggtc gccgcccggg caataaagca gtcgttgccg gcgcctgaac ccaggtcgat
     5221 caccacgtcc cccttttcga tctgggcgaa ctgtgtgggg aggccgcagc caagacccag
     5281 atcggcatcg ggagtatagc cttccacgtt gctatagtcg tcggtcatga tgttgtagac
     5341 ttcgtcggaa cagcatcccg acccgcagca ggaggattga ttggtatccc tgtcctgcag
     5401 ggcgatctcg ccgtatttcc ggcggaccag ttctttgagt tgttcggtcg tttgcataat
     5461 gattatattt atattgcgat ataacgatga atcccggcaa aaaaatttca acaggcgcaa
     5521 ttaccgtcca ctttataagc ggcgaacagc gcgccgatat ccttttgaaa gagtgcccac
     5581 attttcggtt cgatacaata acagacgctg gtgccctcga tcgtaccctg gatcagaccg
     5641 gccgttttca gctccttcag atgctgcgag atcgtcggct gcgccagacc cagctcatcg
     5701 accaggtcgc cgcaaataca ggcattggtc ttcagcagat gctggatgat ggcgatcctg
     5761 gcagggtggg ctagggcctt catcatgctg gccagcttgt tctgcttgtc cgtgaaaatt
     5821 tccgatttgg tgacgcccat gactttatct attcattgca atattacgat aaaataaatc
     5881 aaatccaaat tttttcttga tcggacgcca agcttgcgtc cggaaaaaga aaatgccgtc
     5941 ggcggaccgg cggcagccga ttcaacgttt agttcaatag tttgctgacg gattgcaaca
     6001 aggcagcctt cgtgaaaggt ttcggaagaa agagatcggc gccaatttcc aatgcaaaat
     6061 cgccggccgc cttatccacg ccggagatca tgatgatctt cgtctcggga cagtgagcct
     6121 tcacataacc gatgaagtcg aaaccgaagc cgtccggcag ccggttgtcc atcagtacca
     6181 gatcgggctt attcttcttc aggaagatcg cggcatccgc aagactcttt acatgattga
     6241 tacacaactc tttgtcatcc aaaatgagat tcaggagcaa acacatttct ccctcgtctt
     6301 cgattagtaa cacttgcttg caacggcttc ctgccgggtc tgttgaatgg gaattggcgt
     6361 gcatgggtct tgttgtttgg ttaccgggta cagaacaatt ttgaggccat tccccgatcc
     6421 ttgtgctgcc cgtgacccca ccgccctcaa atggggaaga tgtaacacat aattgtcccc
     6481 cggctcccca atccagcgta cctttccttc aacaattaaa catttaacca tgaaaaaatc
     6541 ctttaccggc aggcgtgatt tcatcaggac cgccgccgca ttcaccggcg ggttcgccgg
     6601 actctccctt cttccttctg caaccatggc aggtcaacag tccggcgatg gtttcgttcc
     6661 cccgccatcc ggccccgcag ccgatgatat taacatcatc ggaccgaagg aaggttatag
     6721 tccgcatatt ggaacgcttg tttcgatgat ggcctggctg cgtccgcaag tgatcggctc
     6781 ggtaaaagac ctgacgaccg aacagctcga ttttctgatc gacgataagg ccaacaccat
     6841 cggagccctg ctcaaccacc tggcagcgac cgatgccttc taccacgagc atacattcac
     6901 cggcgtggag tggggaaaat gggatccggc agtaaccgaa aaattcggag ccgccatgga
     6961 attgggcgac gaaggccgga aaaagatcaa ggggcatgac gtcaagtatt atctcgacct
     7021 cctgtggcag acccgcgcaa gcacgctcac cgaattcaaa aagcacgacg acaaatggct
     7081 gatgaccgtc gatgacaaat ggccctgggg accgaccaac aattactgca aatggttcca
     7141 tgtgtgcgaa cacgaatcac atcacctcgg ccagatcaat ctgttgaaga gtcgcgtgcc
     7201 cggcgtaaag agcagtaata tgtaagggtg ggccggcacc cgtgctcacc atgccgccgc
     7261 tcagtttcac cgtgccgccg cttcaccatg ccccaacaca gtttcaccgt gccgccaggg
     7321 gttcatgccg ccgcttcacc atgccccaac acagtttcac cgtgccgcca ggggttcatg
     7381 ccaccgcttc accatgcccc aacgcagtct caccttcccg ccaccggctt cagctcctcg
     7441 acttttgcct ttggtgccag cgccctgttc acctttccgg cggtgacgga ttcggcaagc
     7501 cggaaggtct tcgataaccg gatgtcttcg ctcgatgcgc cgatcttcac ggtatattca
     7561 ccggcttcgg ctatccaggc agactggccg gtgctgaacg aagccagatc gcgcggcaac
     7621 aacgtaaact ccagcgtctg cgactccccg ggctgtagca gcctcgtctt cccgaaagcc
     7681 ttcagttcct ccgcaggctt gtccatcgag tgatgcggtg cacttaggta cagctgcacc
     7741 acctctttac ccgcaacccg ccccgtattg gtaacggtta ccgtcgctct gagcctgccg
     7801 gaaaagtcag cgctgctgag cctcaggtcc ccatatttat agtcggtata tgacagtcca
     7861 taaccgaagg cataggccgg cttcacattg aacgtattga aataccggta tccgacatag
     7921 ataccctctt cgtaggtaac ctcggccggt accgatctgc cgacgacgga cgtcgtagcc
     7981 ttctcgggga actccttgcc ggggaagttt ttggcggacg ggacatcggt gtaccggacc
     8041 gggaaggtcg tggccagccg ccccgatggg ttcacttttc cgctgaggat atcggcgatc
     8101 gcattaccac cctcctgacc cggctgccaa gccagcagga tggcatccac ctcgtcgcgg
     8161 aaacccgtta cgtcgatgac gcctccgata ttcagcacgg cgatcacctt tttattcttt
     8221 gcatggaaag cagtgctaac cgctgcgatc atgtcttttt cgggaccggt cagttcgtag
     8281 tcatcggcga tcttccggtc attgccttcc cctgcatttc ggcctatata aacaattgca
     8341 agatccgcag catccgcgcg cggaccgatg ctgcccgcat cgaagacata ttcgggtgcg
     8401 taaggagtcg gattcatgat ctcctggatc aacggttttt tgggatgttt cgctgcatag
     8461 tcggccagat aatctttata gcggcgcgca agcccctcat ccacccgata cccggaattg
     8521 gtcaaacctt cggccaacga gactgcgtaa gatttgttca catctccgct gcctgttccg
     8581 cctgctatca ggtcgtaccc gtgattgccg aacagcgcga tggtagcgga gccgttagag
     8641 atgggaagac actggttccc attcttcagc agcaccatgc tttccgcagc ggcttcccgc
     8701 gagatttggg cgtgtgcctt cagatcgggc ttgttcgaat atgcatagcg gttgaccgtg
     8761 ggcgtgtgaa cgaggacgtt caggatgtgt tccacattcc tgtccagatc ggctaccgga
     8821 agcttaccgc tttttaccgc ctccaaaata gtcttcacct ggtcggggtt cccgggcatc
     8881 agcagatcgt tgcctgcctt catctgtgcg acaggatctt ttccgccgaa ccaatcgctc
     8941 atgacgaacc ccttgtatcc ccagtcctta cgaagtaccg tggtcaggag gtcggggctt
     9001 tcggaggtat aaacaccatt gatcaggttg tatgagctca tcactgccca ggggtgcgag
     9061 gcgatgatcg cgatctcgaa attccgcaga tagatctccc tcagggcgcg ttcgctgatg
     9121 atctcattga cggaattgcg atcggtctcc tggttgttgg cggcgaagtg tttgatcgtc
     9181 gcacccactc cctgcgactg gataccattg attatggccg cggcggtcag cccggagacc
     9241 aacgggtctt cagaataata ttcgaaattt cttccgccca gcggattacg atggatgttg
     9301 gcgccgggac cgagaatgaa atcgatgccg tatgacttga tctcttcacc aaaagcgaca
     9361 cccacctttt tcaccagcgc cgtgtcccaa ctggaggcca gtagcgtgcc taccggccag
     9421 gcggtggaga aaagaccctc agccgagtcc tgggcggtca tcatgaacct gtgtatgccc
     9481 gccggtccgt cggccatacc gacagacgga attccaaacc ttgagacggg aatcgtatgc
     9541 cccgaaattg cggcaagttt atcgggcgtc gaatcgttgg cgcgcatacg catgccgggg
     9601 acggagaacc cctttcccac cactactttc accttttcct ccagcgtcat ttgtttcact
     9661 atatcggata ccggatcgtg gccgagtctc ggcggcgtcg gttgtgcgtt cgagggatgg
     9721 ctgctataga ggataacaga cagcgtaagc aggaagaaag ggagccttct catatcaatt
     9781 ggtatttatt attccgtatt tttgacggaa tgaaggtatg ggaagttttt tgaataacca
     9841 aacctttaaa ttcgcctatg gcagatacat tgatagacga catattcacg catgagcggg
     9901 actacctgca caacgtaagc atagacaacg tgatcttcgg ttaccacggg aaggaactga
     9961 aggtcctgct tcagcagcct ttcggcgccg gaaagtggac ggtcaccggt ggctacatcc
    10021 ggagaacgga atctatcgaa gcggcggccg ccaggatcgc cacggccagg acaggattga
    10081 aggatctttt tcttcagcaa ttccgggctt ttggcaatcc ccggcgaaca gccgaaaatg
    10141 gatttactgc cgcgcagttg tccaaactta ccggtaccga ggtccggcca aacgcctgga
    10201 tcttcgacta tttcgtctcg gtggcttttt atacactgac cgaattctcc aaagtgcaag
    10261 tgaccaaatc gccgctcgaa gcggactgtg catggtggcc ggtgggcgat cttccaccga
    10321 tgctattcga ccacgacctc atcattaaag aagcgctcaa cgccatgcgc attcatatcg
    10381 cacattttcc catcggttac cagttgctgg agactaaatt taccatgccc gagatcagga
    10441 gcctctatga gacgatcctt ggccggtcgc tggatgaccg gaatttctcg aagaagctca
    10501 tggccaccgg catcctcaca aagctggaag agaccaggaa gataggcgcc cataggtccc
    10561 cgtacctgta ccggtttaac aagacaaaat atgaagtggc gctgaagacc gggattgagc
    10621 tggctttcta gctgccgccg atgtgccgcg cccggaaatc cgaaggattt tcgccggtca
    10681 gctccttaaa aagcttatta aaataagaaa ggctgtccaa ccccacggca tagcaggtct
    10741 cgcccacctt gtggttttgc atgagtaggt tcttagctct ctccacccgg tattggttca
    10801 caaacgccgt gaaggtcata ttcgtctgcc gtctgaagta cctgcaaaag gcaggcgtcg
    10861 tcagattcac cttccgcgcc acgacattga catcgggacc gcggtcatag tgcgcggcga
    10921 tgtattcgta caccgaaccc atgcggatct tgtctttcag aatgaagctt tgactgtcca
    10981 ggtcgtcgtt cagcctcacc cactcccgcg aatccgccag ccgctgcagc actccgataa
    11041 gacgtaacaa ctggtccagc ggtgtcatat cactcatttc ttttatcaac cccgccacct
    11101 ccgccttcgt cttgccggaa aatgcgatgc cataggccgc ctcatggaaa agcttcgcga
    11161 tcccgcccaa ctcgggggcc accccgatcg cagtcccaag gaaatccgtc cgcagttgga
    11221 tgacgatctg gtgatactcg cttcgaagac ggtagtcgaa gttcagatgc ggcagatttc
    11281 caccgatgaa taccaggtcg ttttgtgtat aggtggagat atgccgcccg acatgccgga
    11341 tgccggcgtc cgcctctaca tagaccagct cgaattccgg gtgataatgc cacaggaacg
    11401 tattgcgcag acgaggcgag aataccttga aggacttgcc ggtctcgaaa tcaatcttct
    11461 ctagctggat ggttttcatt atatctaata tacaaaaaag gtcaatggag agcaaatact
    11521 ggtcaacgaa caggacggcg gcccccatag cccgccctag ctttgtggta taaaatcgag
    11581 attatgaata tgacacccgc tgccggcgca atgacaccca ccctcgacgc gcacaaagag
    11641 atccccggca atccctccac cgccaaatcc agccgtctcc gcctcaacga ccgcagcaac
    11701 ggcgaacccc ttcgcgtcct caccgaagag gactggagat tctggatcac caacggctat
    11761 atcgtcatta agcatgcggt gccccgcgag caggcacttc gcctcgccga ttatctctgg
    11821 aagtacgaag acaaagaccc caatgacaaa accacatggt atcgcaaacc taatgtcgac
    11881 atgcaaatga aggagctcgt caacaccggc atggtagaga tatacaacca tcagtacctc
    11941 tgggataatc gtcaatatcc gacggtccac caggccttcg ccgatatctg gggaacggaa
    12001 cgcctatggg tgaccatcga ccgcgccaat ctcaacttcc cgcaggcgcc cgggttcgaa
    12061 tacaagggat tcatccattg ggactacgat cccgaaaccc ggccgcaaaa tgtacagggg
    12121 gtccttgcgc tggcagacca gacggacgag aacatgggcg gctttcaatg tattcccgat
    12181 ctgtttcgta cttatgatac ctggaagctc acgcaacccg aagaccgcga tcattacaaa
    12241 cccgacacga ccggtttcga cattgtcaag gtcccgctcg aagccggcga cctgctgatc
    12301 ttcaacagtc ttcagccgca tggtatccgt ccgaaccgca gcggggataa agtccgcata
    12361 gcacaatata tttcgatgat gcctgcgcag gaggaaaatg atgaactgcg ccaatggagg
    12421 atcaatagct ggcaaagccg cgatccgatg gagggttatg catttcccgg cgatccgctg
    12481 cagcgcgaga agcaaaatcc cgtggcgaca ttgtctccgc tcggggagcg gttgctcggc
    12541 ctgacgcgtt ggtaggacgg cccgatctat tgcgtcccgg gcttgaaccc cgctgatccg
    12601 ctcaccgcgt cagctttacg ctaacccctt tgcgtaacgt gttatgcacc tccgcgaccc
    12661 cgtcggtggc gcggatcaat ttctcgatgg tctcagccgc cgcatccgag cttattttga
    12721 cgttgtaaca gaaattatct cccggctcgc cctccgcacc gaaggtgccg gtgcattcga
    12781 cttccacgcc cgtgacagga atattctttt tcgccgcctc gcgatagata tcattgcaat
    12841 agcaggtggc gagcgccagc agcaataatt ctccgccatt gatagcagac ccaaagccgg
    12901 aaggtttagt cggaagagcc agactcttgg cgctgtcatt cgtctgcacc accacgtcct
    12961 ggcgatgatg tgtgctggtt agtttggcgg agattttcat gggttcaaat gtaagggatt
    13021 gacggaatcc cgcaataatg gctaaattgc ttatcgctaa ccataaaaaa catcttatgc
    13081 aaaagatcag cactttcatc tggttcgaca acaatgccga ggatgcaatg aatttctatg
    13141 tctccatttt caaagactcg aaagtgatca gggtcgcgcg caatccgccg ggagcacccg
    13201 ggccggaagg cgccctgctc gtggcatcga tccagctcga aggacaggaa ttcatcctga
    13261 tgaatggcgg ccccggacat ccgctgaccg atgcgatctc tttgacgatt aattgtgtct
    13321 cccaggaaga ggtcgatcac tactgggaca ggctgctcgc gggtggcggc accccgatcg
    13381 cgtgcggttg gctgaaagac aaatttggac ttcactggca ggtgacgcca acgatcctgc
    13441 cgcaactgat cggcgatccg gacaaggcta aagcgggccg ggtcatgcag gccatgatga
    13501 aaatggtcaa gctggatata ccgacgttga aggccgcggc ggacgctaaa taggaatgcc
    13561 ggtcattttt ccgggtatcg gatctcggcc ctgaacctca cgtggatgtg actgcaatcg
    13621 attgcagcaa atctctttta tcgtatatta gtcgacaaaa tcaccccccg catgacctca
    13681 ttccaatctg caaaaagact attgctcttg ttattggcgg gtttctccgg attgacggct
    13741 gccgcacaat atagaaatgt gtttttggaa gcgggatacc gacgctcgga tatcgactcg
    13801 aaagtggaca aggtctaccg cgacttgttc gaaggtccca ataaagtgta tttcgaggtc
    13861 ggcgattcga tggggtatgt gtccgacctg aaaaaccatg atgctcgtac ggaaggactt
    13921 tcttatggga tgatgatcgc cgtgcagttg aataagaagg acgtgttcga ccggatctgg
    13981 cgctggtcga aacgctatct gcaacacgct gacggtccac gcaagggcta ttttgcctgg
    14041 agcatcgatc cggcaaccct gcacaagaac tcggaaggca cggcatccga tggcgagctg
    14101 tatttcgtta cggacctgct gttcgcctcc aaccgctggg gcaaccgaac gggcatcgac
    14161 tattaccacg aagccaggac gatcctcgat gacatgtgga aaaagaatgg ctccggcggc
    14221 atttatccct ttatcaatac ggcacacagg ctgatgacct ttacgcccga aggcgataat
    14281 tataactgga cggacccctc ctataatttg ccggcatttt ttgaggtgtg ggctatgtat
    14341 gccaaagacg gtcacgagca attctataaa gaatgcgcgg attcggcacg tgcgttcctg
    14401 caccgagcct gcgatccggt cactggtctg aatgccgatt ataccgaatt cagcggagcg
    14461 ccgcattcca cccggtggat gccgccaggc ttccggtatg actcctggcg cgtgcccatg
    14521 aacatcgcga tggactacaa ctggtttgga aaggatacgg cctggcagcg ggattatgcg
    14581 agacgttttc agtctttcct gctgtcgaaa gggctcgatt ccttcccgga tcaattcaat
    14641 accgacggct cggcgcccgc tgccattctt caggcaggcg gatttaaaaa gctaagacat
    14701 tcgctgggac tgctggcgac cgcggcctct acgacattga tctataaccg ggatcactcg
    14761 tttgaattcg cgcgcgcctt gtggaatgcg aagctgcaac cgtatgcgga cggatatttc
    14821 gatccttatt acgatggctt actatatttg tttgcgctga tgcatctcag cgggaaatat
    14881 gggatcatac gaccccaggg gcgctaagcc tttcaatgga atacgaaact tatataccgt
    14941 ccgatccgct gaaaccatac atccggtcgt ttgccattca ggagacggcg gcggaggcga
    15001 catacagggt acttcccgac accggcatcg tcgtgggctt tcagtataag ggtagacttt
    15061 tccgtatcga cgaaggcact ccttcagccc tcgatacttc cggtctttca ggattggccg
    15121 atcatggccg tactttccgg aatctgtccg gcaccggaac catcctggtg ttctttaaag
    15181 aagccggggc cgccgccttc ttccgccagc cattacacga agtcttccgc gaaagcgtct
    15241 ccctcgacaa tttcctgcta cgatcggaac ttctttgtct cgaagagcag ctcatcgagg
    15301 cgccgtcgaa tcccgaaagg attgcaaggc tggagaagtt cttcatcggc cggatgtccc
    15361 gtacagagcc ggataagctg gtgcttgccg cgctggccct cattcaccag accaggggca
    15421 atatccggat cagggacctc gccgtgcagc tgcataccag ccagagtccg ctggaaaaac
    15481 gattccgggc ggccgtaggc gcctctccga aaaaattcgc gtcgatcgtc cggctgaaga
    15541 atgtcctgca gcaatattcc gcgactaata gcctcaccga gctgggttac gaggccggct
    15601 tctatgatca ggcccacttc attaaagagt tcaaggccct cacaggtgac acacccgagg
    15661 attttttcaa aaaggaatag gaaaccattc cgcagccaac cgacaatctt ccgcagccaa
    15721 cccgaaacca tttcacacgc aaccccaaat ctttcgcaca cgctaacaaa acgatttttt
    15781 acaattccga ccgccggcgt cgcgccatct ttgtcctaac ataaaatttt taccaatgcg
    15841 acggatgaag acctacgatg ttcctcacaa aggcctgcgg aatggcctct cccaactagc
    15901 cctgatggcc ggccggaccg attattccaa cccgatggag gtgacgcagc tgcaccagct
    15961 cggagcccgc cttttcagta ttctcacgat ccacgccgaa gatgagaatg ccgtcaccct
    16021 cgctcatctc gagacccgct gtcccggcag tacccggcat gatctcgatg accacgaaga
    16081 gatccatcgg acccagcaac aactcgaaaa gcagctcgac gccatccatg ccggcgcccg
    16141 ggacgggaaa gacatgaccg aagccggcgc ggaattctac ctggcgttca ccgaatttca
    16201 aagtgaatat ctcgggcata ccgcggaaga agagcgtgtg acccagccgc tgctttggca
    16261 gtattttacg gatgaagagc tggccgcaca ccgcggacag atcatggcca ggaatccgcc
    16321 ccggaccctg ctcacctggt tcgaattcgt cttccccgcc caaagccatg cagagagggt
    16381 agggcttctc accgggttcc gaaaaatggc cccggcggga ttctttgccg aagcgatgga
    16441 ggtgatccga aaagcccttt ccccttcgga gttcggacgg ctggaggatg cgctggcaat
    16501 ttgatgctgg gcccgaggcg tctgaagacc actcccaacc cgcgaccggc agccgtctga
    16561 agaccactcc caacacgcga cccgcagccg ggccattgcc cggttttttg gcgtatattc
    16621 attaaatatt atctaaatat gataaaaaga tcaattatct ttctggcgct tgcgatatcc
    16681 ttcggttttg gcttcgcatt ccattcaata ttgaccaaca caaccaaaaa acaagcaaac
    16741 atgaaaaggg taatcggact cggcggcatc ttcttcaaat gcaatgaccc gaagaaagtc
    16801 accgaatggt atcaaaccca tctcggattg caatcgagcc catacggagt aaagttcgac
    16861 tggaggcagg acggagactc gacaaaaaag ggatttaccc tttggacgcc cttccccgat
    16921 aagaccaaat acttcgagcc ctcgtcaagg gactttatga tcaactatcg ggttgaaaac
    16981 ctggagacgc tggtcgaaga gcttaaaaag gaaggcgtga cggtgttgga taagattgaa
    17041 acctacgact atggtaagtt cgttcacatc cttgaccccg aaggcaacaa ggttgaacta
    17101 tgggaggcga tcgacaaaca gtagcgcccg cgccgtcacc ctccggcccg catcatcacc
    17161 acaccccccg cgccggtaac cctccggccc gcatcatcac cgcacccccc gcgccgataa
    17221 accctgcagc ccatttccct ttttactccg ttcgcaaact ccttaccgga ttattcctcg
    17281 ccgctcttaa cgcgaggaag ctgaccgtcc ccagcgcaat gacgagcgca gctacgccgg
    17341 ccaccacgaa cacccaccag ctgatactca ccctgtatgc ataatcctgt atccaccgat
    17401 ggagcgcgaa ccatgccagc ggccaggcga tcagattggc gatcaggacc agcccgataa
    17461 aatgccgcgt tagcaggacc aggacgtctt ttaccgaggc gccgagaacc ttacgtatgc
    17521 cgatctcttt ggtccgccgc tccgtagaaa aagaagccag acctaacagt cccatacacg
    17581 aaatgaggat cgccagaccg gccatgatcg ccaccatttg tgatatctgt gtatcggtcc
    17641 tgtatagctg cctgaaatga tcgtcgagga actggtagct gaacggatac tccggaagga
    17701 tctttttcca ggtggactca ataaacgcga tggcctgctc cgccttccgg ccgttgatcc
    17761 tcacggatac cgtattgaag gagcttgcac cctgatcgag caggaacatc ggttcgatct
    17821 tatagtgcag cgaattaaaa ttgaagtctt tcgaaatacc gacgatccgg cccagggtat
    17881 caccgaaatg tttaccgatc agccacgaca gcggcgttcc gggccggtca tgtagcaggt
    17941 ccctggccat cgtttcgttg atgatgaatt cgttcccgta tccggaagta tccccggtga
    18001 aattgcgacc cgcctccaga gatatcttgt aaacggtaag ataatcggga tccacgaaga
    18061 tccccggggt gaacagcaca cgcatcgggg cgtcgcccgg ccagaacccg aatcccagcg
    18121 ttgccaacgg acctcccaaa tggtcctgcg cgcccgtgac ccccgttatc aacggactga
    18181 ctgacagctc ctctttcagt aaagcatatt tacggtccgt aattccatgc agtctcatgg
    18241 taattacctg gtcgcggtcg aacccggggt cttgcttctc catgtacatc agctgccgga
    18301 aaataagaat ggtaactatc accaggaaga tagcgccggc gaattgcgtt accacaagta
    18361 tattccgcaa aatgcctttg ctcctgcccg actcgctgcc gcctttcaac accttcaccg
    18421 gggaaaagga agacaggtat attgcagggt acaaacccga caagatacca agcaggatgg
    18481 tcccgatgaa cactcctgcc agcagcccgg gatgcagata gaaaaggggg ctaagctgcc
    18541 ggccactcag gtcatcgata taaggcaaca ccaggcttac gatcgccagc gcgagtacca
    18601 gcgagatcag ggataacacc accgagtcga tgagaaaccg gacggccagt tgcgggcgcc
    18661 tggcgccgat cgttttgcgg atgcccacct cttttgcccg tccggccgac tgggctgtag
    18721 ccagattcat gaaattgatg caggcgatga gcagcaccag cagaccgatc cccatgaaaa
    18781 tatcggtata gcgtcggtcg aatttctgga aattgatatc gtcgtcaccg atatcgacgg
    18841 cattggcatg aacgtccctc aatggcagga ggaagagatc atgacggatg tgctggtccc
    18901 cgatcttata ttttttcagg taagtaggga atttcttttc cagtgcagca atatcggtat
    18961 gaggcgcgag ctccaggtag gtgcttaccc cgtcatgcga ccgcgtcctc aggacggtgt
    19021 taaaagactg gagcgcgtcg aattgaaatt gggaattcgc gggtacgtct ttcagtatgc
    19081 ccgtcaccgt ataaagaacc gtatccgtat cgaaatgcga aacggtctta ccaataggat
    19141 ccacagtgcc gaacaatgtt ctggccgccg tttgtgtcag gacaatgcta ttgggctttt
    19201 gcagggcagt ctgccggtct ccttcaacga gcgggaagtc caacagctga agaaacgagg
    19261 tatccacgaa gtaggtatat gggaagaata ccctcttctc cccatacgtc atctcgtatt
    19321 ggctattcag atccacacgg ctgtagttca ggatctcggg aaattcgtct tttagcgtgg
    19381 ggcccgtcga aatgcccgtg atcgccgact tctcagagtt tcccgtagcc gtaaatttca
    19441 gcacttcatt cagccggtac agatttttgt gatgcatgct atcgaagctc ttttcgtaga
    19501 atacgaatag caggatcacg acgcacgcgg ccatgccggt gcccagcccg attatattga
    19561 tcgcagaata caacttattc ctggtcaggt tgcgccaggt aaggagcata taatgtttga
    19621 acatattggc agatcgtttt actcatgtta cctgtttccg ggtaaagaaa atgccaaaag
    19681 tccaatcatc ttaaaatcaa atcaatatgt attttaggtg gcaaccggac tgttcggata
    19741 tgaacaccgt gcgaacgtta atgatctggc gctcgcccgg cgtttgaaca gatcgctcag
    19801 tgaccgtcgt ggtaagtccg tggccttcct tggtcatcca cataagtggt cttatgcgcg
    19861 ccacccaggt tgagactcac ctggaacagc ggctggccgc cggggtgttc gatccgggtg
    19921 tacgaatagt ctctgccatg gggataccgg tgttccgtcc tcctcctgac ttccgacggc
    19981 acatcccgat catcataagg aatgtgagaa tcgatatgcc tgccatgccg atcataatgt
    20041 gccgtcattt ccccacggtc tttgggacta cggtccgtga agtcggcgtg ccactggcct
    20101 cccgactcac tccaacgggt atcgcccgcc tttggataat ctctttgaaa tgagttccgt
    20161 acgttactgg gaacctgttg ttggttccgg tgttcctgcg ccggcgcaat ctgggcaatg
    20221 gccagcgccc ccaataccaa tatgatactt ttcatgatgg ttcattctaa ggttaacaat
    20281 gtgattcatg accagacgga aaatttcatg ccagccctgg cccggggttt tgcccatgct
    20341 catcggagac gataggaacc tgggtcagat gttccctgaa gggggcatat ttgcctcggg
    20401 gacatcctcc aggctgtaat agaccggcaa gccgcgttca cgcgcgagtt taacatcatt
    20461 atcggcgcct ttcgacgagc cttcgagccg gaagacggcg tcgcacttct ccaacagccg
    20521 gtgagccgtg gggtactgga tcgtgtccca ggcggcatcc cccggtctga cggaacctgc
    20581 cagttttatc aatggtaatg ctgcccattc gccgatcatg ggcatatgtc cctttctaaa
    20641 gaggggtaat gcgacggact ccaggtagtc cagattcttt ctcatcagac cggggtcatc
    20701 gccggtgccg ctcctgtagg gccctgcaat caatatcaac atacggttgt tttttccaaa
    20761 ggacggaagt atttatataa aaggacttaa ggtactttaa gatttcatcc ctcttacggt
    20821 gctcaggaat tcgggcgtga tacccagata ggaggccatc tgttttaatg ttaccacttt
    20881 cgccacctgt ggatattttc tcaggaaact gtcgtacttc tcctttgccg ataaagacag
    20941 gttctctata acccttaatt gttccctcgt ataggcgttc tgcatcaata tccggaagaa
    21001 tcggtcgaac ttcggaactt tcttatagag ctcatcatga ttcgtcttgc tgatctggac
    21061 aatacagctc ttttcgatgg tctctatata catcatcgca ggccggtggt tcagataaca
    21121 atacatatcc gttatccacc aatccgccac ggcaaacagg atcgtagatt cctttccggt
    21181 cttgtcgaca tagtaagctc tgagcgctcc cgaagaaaca tagctgagct ggttgcacac
    21241 ctgcccttcc ttcagaagga gtgttttttt gggtaactcg gaggatgtcg tcagcgacaa
    21301 aaaatattcc gtttcccccc ggtcgagggt aatgtgtttg gcgatattat ccaggatggg
    21361 ctcatgttcc attggcgctg tgatttatcc taagatactg aatctagcgg tgccgggggg
    21421 gcgccttgtc gctcctgcag agcggacccg ctatgccgac agccataacg ccgcatcgcc
    21481 ttcatgatcc agctgggtga catccggatc gaacgttatc ccatgaagcg tcagctctgt
    21541 tttctgcaac agctgaaaac cccgcgtagt cacctgcccg ccgttgtagg cgcgccaata
    21601 gtgctggaag gaggtgtcat tgtatagttg atccggtgta agtcccgtac cgtagccgac
    21661 atagaggccc ggcacataac ccgcggcatg cactgcgtta taccacgcct ggcagtagga
    21721 gacgacatcc tgcgcgggag aaccgtcagc cacttcttcg aggtcacacc agacattcat
    21781 accctttggc agcttaacgg tctgcgcgca ccaggtggcc gcgaaattcc catacatcgt
    21841 ccccagattg gtggtcggtt tccagccggg cagggagacg tgctggacag gcatcagggc
    21901 aagcccggcg gcgagaatac tcagggcctc ggcattggtg atgttgccgg gcgcgagcgc
    21961 ggccgtcctt ggcacatacc ggatgcagaa attatagccg gcatttttaa aatccaaggc
    22021 ctgatccgcc gtcaaaacgt gattgacatc gaatcccatg gctccggccg gagcaggctt
    22081 gacgctgccg ggtaaagttt gtgtgggcat acaaagagtt taggtttggg ccaatttaaa
    22141 gcaacgtcaa agtataaaaa tgggtgtttt tcccatattt ttagcctata atttaggcat
    22201 tatgaaaaaa ttgcttgcac ttctcgcgtg cgtcatggcg gcaggcctgg cgcaagccca
    22261 gccctctagg ttcagccgtg aaaggtatat cgaaaatggc gatacactgt cctaccggct
    22321 gttgttcccg gatgccaatc ggcagaggaa atatcccctc gtgatctttt tgcacggctc
    22381 gggcgaacgg ggcagtgaca acgacgctca actgaagtgg ggagtaatga attttgccac
    22441 cgaccggaac atgatggagc atccggcgct cgtcatcgcg ccgcaatgtc ccgaacacca
    22501 gacctggagc agctttacgc ccgataaaaa caagatgggg atgacgctga atgccgagcc
    22561 cggcaagccg atgcaattgc tcatcgggct catccgccaa ctggagaaga cgatgccggt
    22621 agatacgaac cggatatata tcaccgggtt gtcgatggga ggatacggta ccttcgatgc
    22681 gctggaacga tatcctcatc tttttgccgc ggcggtgccg gtgtgcgggg cgggcgacac
    22741 ttcacgcgcc gcgtccatcg cccatatccc gatgtggatc taccttggcg cggaggaccc
    22801 cgcagtcaac cctttatacg ggctggctat gttcgaagcg ttgactaacg ccggcgccca
    22861 tccgggtttc acccagtacc ccgaagtagg gcatttttca tggctgggcg catacagcga
    22921 tccgttgatg atggagtggt tgttcagaca gcataagtaa aggcacctac tccgcccgga
    22981 gactcttcac cgggttggcc atggccgctt ttacgacctg caaactgatc gttgccatcg
    23041 cgatcatcac cgccaccagc gcagacagca caaagaccca ccaatcgata ttgatcctgt
    23101 aagcgaagtc ttgcagccac ttatgcataa agaaccaggc aataggtatg gcgatcagcg
    23161 cggcgacgag caccagcttc aaaaattctt ttgaaagcat aaggatgata ccgccgatat
    23221 tcgcgcccag tacttttcgg atgccgatct ctttcaccct ttgttccgcc gcgtagatgg
    23281 taagacccag cagaccaagc gtcgctatga atatggcgag cagcgagacg atgataaaaa
    23341 tggtctgtct tgttctgtct tcccgataca gcgactccca ctgtttatcc agaaaatgat
    23401 attggaaaag accgccctga tccatccgct gcatgatcgc cttcatgctc tttagcgtgc
    23461 cgctgggatc gccgcccaca agtcttacgg taaaatggcc ataattttga gtaacgtccc
    23521 gtttaaaacc aagcatcatc ggggcaaccg gctcgtgcaa tgactgaaaa ttgaaatctt
    23581 tgacgatccc tgcaatcgtc agagaaagcg gtttttcgat cgcggaacgg gtgtcaccgc
    23641 cggtctttgc ggatgagatg gtgatcacct ggttcctggc atccgttatt ccaagctcct
    23701 ttgcggcagt ttcatttata agcaccgacg aagaatctcc acttcctgtc gggaaaaagt
    23761 tccggccttt tattagtggt atatcatagg tggaaaggaa ttgcccatcc acaccgaaga
    23821 aataaatatc cttgccggtt gcggagttct ccccgttcat attgaccttt acagagggga
    23881 tcgtcttcga ttcaccggga accatcgacg ttgcggtaac ggaactgacc cgggcaagct
    23941 ttgaaaattc attttttatg gttcccgccc tacggccgaa tataccgccg tcgttatcga
    24001 tcacgacaag tttatcctta tcgaaaccca agtccttctt atttacatat tgcatctgct
    24061 gataaataat tatcgtggct gcaataagga cgatcgagat catgaactgg aaaaccacca
    24121 gtgagcgccg cagcgaaata ttaccgcgcc ccagttgtat tttgcttttc agcaacgcga
    24181 gggggttgag gcctgattga aatagggcgg ggtatagacc cgacaataaa gtaacgatcg
    24241 caaggatcga taagacaccg gtccatatcc ggtaatcggt gtgcgcgttg agggtcaggt
    24301 gtttttccgt aaacgaattg aaaagaggta ataacagatt gaccagcacg agcgacagca
    24361 ggacggaaaa aagggtcgcc atcaatgctt ccgcaaggaa ttgctttatg agcgtcgctc
    24421 tcgacgcgcc cgaaactttc cgtatcgcga tctcctttcc cctgtttgta aatcgggcgg
    24481 tcgcgagatt catgtaattg atacaggcaa taaagatgat gaagcaggcg acgatgagaa
    24541 aaacgtagat ataataaata ttgccccttt taccggaatt tccttcgata tcgttggaat
    24601 aaaaatgcac gtccgtcaat gcctgcagtt gaacaaggct cttaacgcct gcctcggcgt
    24661 tgtgattggc ggcaataagg ttgtcgagct tcttatttaa agccgggata tcggtattgg
    24721 gcttcaatag gaaatacgtc gcgaaagcgc cggacgtcca gtcgttggcc agccagtctt
    24781 tcggtagtga cacttcggac accagcagat cgaatgaaat gctggaattg gcggggaagt
    24841 ttttaaggac gcccgttacc cggtatggaa cggaatcgtt atcgaaaaat aaggatcttc
    24901 cgacaacgtc cgacttgcca aaaaactttt tggccgtcgt ttcggtcagg acgatcgatc
    24961 tggggtccgt caacgcgctg ctccggtcgc catagatcag gggaaagctg aagacatgta
    25021 gaaaaccggg attgccggca ataaaatctt cctggaagac tcttgatctg ttgtcacccg
    25081 atgcgacgct ggcacgccaa tataccgaga tacgggcggc gtcctgtatc tcgggaaaac
    25141 tggtagttgc ccttgcagag acctgggagc ctgtgccggc tcttttcgcg gttttaccat
    25201 ccggcgacgt tctgttctcg accacccggt agatattcgc ggcgtcgtcg tggctccggt
    25261 cgaaagtaaa ctcatcgaaa acatacaaag cgataagtat gaaactcgtt aagccgatgg
    25321 tcaatccgac gatattgatg aaggaagaaa ttctgttctt ccataaattt ctccaggcgg
    25381 tgataaaatg gttcctaatc atgagttagg cgtaacaagg cccgtaccaa ttggagaaca
    25441 tccttgtagc aaagaaggac aaaaagtccc cggcgccgca agctgtatcg aaaccggaca
    25501 ctgctgtatc gaaatcggac atgccccgct ggccggatca gctggtcagc tgatactcga
    25561 ggataataac gccgttgtcg agcgcgcgtg actgtttgag cccgaatttt tttagttccc
    25621 ggttgtcctc gaatagccgc aacccgccgc caacacagac gggataaacc ataagatgca
    25681 gctcgtcgat gagatcgtga tgaagcaggg ccttgacgag tgtggcactc ccatagacga
    25741 ggatatcgcc cccatccgtc ttcttaagcg aggcgacatc ccccgcgtcc gcatcccccg
    25801 ccaccccgcc tcccgccacc ccgcgcgccg cgacatcacc ccccttcaca tcacgacccg
    25861 tcatcccgct ctctgccgcc ccgcgccccg cgacatcccc ccctttcacc ccccgcaaaa
    25921 tatgcgtgtt gttccaatcc acctttttca ggcttcgcga cacaacatac ttcggcaact
    25981 gattcatgcg gtctccgaac ccgtttccgc cctgacccgg ccagtacccc gcgaacactt
    26041 cgtacgttgt tctgcccaac agcaacgcat cgacggctgc cagctcgtcc atcttatact
    26101 ttccgccctc cggatgaccg aatttagtct gccagccgcc atgaggatga ggagtttcat
    26161 tgccgccggg cgcctcgacg acgccgtcga gtgtgatgaa ttcggtgacg ataatttttc
    26221 gcatggtttt ctatttcagg gtttcaaaag ttggtccagg tagttcaacg tcatggtaaa
    26281 gccttctttg aagcccattt ggatgagttg ctccaggtcg gcgagggttt tcagttttat
    26341 gacgatggat acggatgtcg ttccattccg ctcgctgaaa ctcaaatccc aatccgagcg
    26401 tggcatcgcc tcatttatat tttcatcctt gtccgcaaag gcatcctggt atttgaaatt
    26461 ggtggtcgga ctgacagagg tatacttctg cagcgaccag aattcttgcc cttcggggct
    26521 taccatggca taaaatctcc gtccgcctgc cgtaaagtcc atatgttttg tgcgggatat
    26581 ccagggtttg ggcgcccacc actgatcgag gatctcctgt tttgtaaacg catcccacac
    26641 cttcgaaaga ccggccgcga agtccctgtt tacaaatatc gaatttgtcg tcctgtcgac
    26701 ggaaaagtcg aattgcaaag tgctgttcat tttttctgtt ttttaatagt ggataatact
    26761 ttgtcgagct ggtcgaaccg ggtttcccag atctttctga attggctcaa ccatttgtct
    26821 atctctttca ttttttcaat ttcgagtgag taataaattt ctctgccctg atattcttgt
    26881 tttaccagat cgcattccgt caggatgcgc aggtgtttgg aaacagcctg cctggtagtg
    26941 ttgaagtttt cggcgatcgc attcggcgtc atcgcctgca acgcgatcag cgcgatgatc
    27001 gcccgtctgg tcgggtcggc gatggcctgg aatatatctc ttctcatgcg gggctttttg
    27061 aattgagaaa ctgatcggtt gcaaatatac gtgcaactat tcggttgcgc aaatcttttt
    27121 ttgaaggcaa ttgccggcgg cgcatcctgc cgcaaaccag aggccaaagc gagattcctt
    27181 aactttgtga cccatatgaa aaagctcctt tgttatatcc tcctgtccat catcgctgtc
    27241 tgccccgcat tcgccgtccc tgccggcccg aatcttccca acagtccgga tggcccgaat
    27301 gttcccaaca gcccgggtgg cccggatctc cccaacggcc cgggcgcccc gaagctcgat
    27361 acggcaacct tcgctacggg ctgtttctgg tgtacgcagg ctacattcca gcaattgcgc
    27421 ggggtaacca gggtcactgc cggcttcacc ggcggccata ccgttaagcc cagctatgaa
    27481 gaggtgtgca cgggcactac gggccatgcc gaagcctgta atatcgtttt cgacccccgg
    27541 gttatttcct ttgacacctt gctggaagtc ttttttaccg tccatgaccc tacccaactg
    27601 aaccggcagg gtaacgatgt cggtacgcag tatcgttcgg ctgtgttcta tcataatgtg
    27661 gttcaaaaac aaaaggcgga atactacatc aggaagatca acgaggcaaa ggcctatccc
    27721 agacccgtcg tgacccgggt agtcccgttt accgtgttct ataaggccga agactatcac
    27781 caggattatt acaaccggaa cgggaatgtc ccctattgta aatatgtcat tcagccagaa
    27841 gtggaaaaat tcagaaaaat attcgccgat aaattgaaaa cgacccatga ataagatcat
    27901 gcgaaccttc atcatagccg ccgttctggc acttggtgtg gccgcccggg cctttcccca
    27961 gcatttgaaa tcccccgagg ggcatccgaa caatccttat tattcgaata cggatacgaa
    28021 caagctccat gtatcggatg cggaatggaa gaagatcctg tccccggcgc tgtatgctac
    28081 cgcccgggaa caggccaccg aaaagccgtt taccggacga tattgggacc aaaatgccaa
    28141 agggacctat tattgcgcag tgtgcgggaa taagttattc cggtccgacg ccaaattttc
    28201 gagcacctgt ggctggccga gctttttcga acccagccgg aagaacagcg tgacctacct
    28261 gcaagaccac tcttatggca tggatcgtat agaagtgcgt tgcgcccgct gcgggtcaca
    28321 tttaggccat attttcgacg atggtccacc gccgacccac aagcgatact gcatgaattc
    28381 catttcattg gatttcaagc cggataaata gcggcattat ccccttttca ggcatgggcg
    28441 tatttaaagg aaaaaggagc gtatggaaat acgctccggt ctaacgattg cttgccatat
    28501 gaaaaatgtt accccgcgcc ggcgcaggct ccgttttcga ggtcgcaggc ggaatcgaac
    28561 cgccgtgaaa ggttttgcag acccgtgcct gacctctcgg ccacacgacc atcaacgcag
    28621 caaatgtaca ggatcgcaat tagtccacca aatttataga ctaataaatt ttaggccacg
    28681 ctgacaaatg aaccacaatg ccagcctgca actatcttgc aattcgttcg gaataagaga
    28741 atgaaataat gaaggctaat ttccctgaag ctgcttcatc agcttgtcgg cctgctcctt
    28801 tgtgcggatg atcttatgaa gggaggaccc gttgcgcaca gcagtcccac gcttgacctt
    28861 ttgagggccg agactgcgtg ctttctcagt aagtgacctt atcttatctg ctatacttcg
    28921 ggtcatactg taaagataat aattttttgt gaattttatc cggcttacct ggcggtgttc
    28981 agtctgaaaa tgaagtaatt gtacatccaa tccgaacgga tgatcctgcc tctggaaatg
    29041 gggtcgaggg aggtataaat gtctttaaaa tcaaacgggt tatcaaattg gttttttccg
    29101 aaccggctga tccggacata atatttgagg ccatgcatga tgaagaattg agccaggtag
    29161 ccgaagttgc gttgaatgaa gcggtaatat agataggccc gggcattatt cgagccgtcg
    29221 ataccaagaa agtgggaggg attactgctt agataggtta gcgccgtaaa tagaatggtg
    29281 gaaaatgttt tagaataatc agaatgttca atttcagcct tgtcatcgac gagccccctg
    29341 gaattcagcg ggccgaatgc caggttgtag acatccggaa gaagggggtg tggttgattg
    29401 ctgatttcta cacgcagagg aagtgaagtg ccatcagcca attgagattt aaatgtggta
    29461 gtacgaaggt ccggggtcac gggttcaatc tcgtatatat gatcgaaatc aatattgacc
    29521 gtaagcgcca tagaattaag gttaataggc gcaaaaataa ctcgctaaaa tggaagcagc
    29581 cgaaattttg taaaagccct gcgaatatcg catgacttac cgtgtaaccg aaaaatagcc
    29641 taaattagtc actatgaaac cattgcttgc cgctcccgtc cacatcacgg cagccctcct
    29701 cgtattgtta tacccgaccg ggctctgcaa cgcccagcaa cccgcccccg ctgcgaagcc
    29761 cggcactacc acggtcactg caccgcccgc catcgcgacc gcgcaacccg gcgccaccac
    29821 tgtcactgaa tggcaatatg gcaggaacgg cgcactctcc atcacctacg acgacgcttc
    29881 ccggcaccag ttcaccaaag cattgccatt gatggaacgg ctgaaattac cggcaacctt
    29941 tttcgtcatt accggaccga tcaccggatc gaaataccat ggcagattta tcggccggcc
    30001 cgtgcaggag atcatcaggg aatccgcaac gatccctaca aacgacaata atttcttcga
    30061 acgctgctcg gcagcaggat acctgggcta ccagggaacc atcgcttacc atacgcgcgc
    30121 cggcggcatg tattcatcgg gcagaaaaca acaggcctat cacctgatgg acagcctcta
    30181 cacaaaggtc cggcacgggg attttccgca gggttatgaa ccctgccccg aatacaagga
    30241 ggccgaaggc tccagctgga atgatttccg gaaagatgtc gcggagggct atgagatcgc
    30301 aagtcactcc atcacacatg cgaccatgcc cggcatggac gaggctaata tcaaatatga
    30361 gctggaaaag agcaaggaag agatcaggaa ccagctcggc gacaaatata cattctccac
    30421 ggaggtgccg tatggctacg aggacgaccg ggtgatgcag atcgcttata agatatatcc
    30481 ggcgttacga aaccggatgc cggagccgtt tttaaaggag atagacaggg gaagccggat
    30541 gttgccgcat cccaatgaca aggactatgt ccaatggcag cgcgggccgc tgcatgcaac
    30601 cccgctccca ctgatggaat cctgggtgga caccgccgcc gccacccacg acacctggct
    30661 ggtgctggtc tttcatggca tcgacagtct cggatgggaa tggacgccga tgcccaaact
    30721 ggaggaatat ttcaactata tcaagacacg cgaggacaaa ctctgggtgg cgacgttcgg
    30781 cgacgtcaca aagtatatgc gcgaacgcga ggccgcaaag gtcaccgcga accgaactag
    30841 cgaaaccgcc gccgcgccga aaaccgaaac cccagcgaaa agcagcaacc cggcgagcag
    30901 caacccagcg aatagcggca ctatcattat caccctcacc cactccctcg accccaaaac
    30961 ctacaccctc cccctaaccc tcaaaaccta tatccccgat acctggacaa aacctaccgt
    31021 gatcataaca caaggcacaa cccgacagca cgccgccgtc caaaaagatg acaaaggcaa
    31081 atacatcctt taccaggcaa aacccaatac cgtaaacgta acactccatc catgaagcgc
    31141 gactactaca tcgtaggatt gatcatgctt acctttttcg tgatctcgtt cctgaccaac
    31201 gtgatccagg cattgaaccc ggactttatc accgggtttc atttgagcct gaccctggcg
    31261 gcagtactgc cattcgcttt ttttatcgcc tatggcctcg tctcgatccc taccgggatg
    31321 ctcctcgaaa aatataaaga aaaaaagatc atggtcgcgg gcttcgtcgt cgccgttatc
    31381 ggctcagtgc tcctggcggt gatccacaac tatttgaccg cgatcatctc gctttttctc
    31441 atcggcgccg ggatggccat gctccaggtg gtgatcaacc cccttctgcg gaccgccggc
    31501 ggcgaagaac attatgcgtt caactcggtt ctggcgcagc tgatcttcgg ggccgcgtcc
    31561 tttctcagcc cgctggtcta ttcttatttt gcacagcatc tgaacggtca gacctccggc
    31621 ttgatgggac tgttcaacag catcgttcca ggcaacctgc cgtggatctc gctatactgg
    31681 atattcgcgg tcatttcagt ggcgatgatc gtggtcatca aaacctcccg cttccctgtc
    31741 gtcgagctca aggccgacga aaccgccggg ccctggaaaa cccacctcat gcttttcaaa
    31801 aaaaaggtag tgatactcta cttcattgcc atgatctgtt atgtgggtac cgagcagggc
    31861 atcgggaact ggatctcgaa attcctcgaa acctatcacc attatgaccc gaaaacaaaa
    31921 ggagcggata ccgtagcgta cttctgggga ttgatgacgg ccgggggcgt cctgggactg
    31981 ttcctcctca agatcatgga cagccggaaa ctattgatcg cctttactat cgcggcgctg
    32041 gtctgcctga gcgccgcctt attcggcagc gggccggtgg cgctgatcgc ctttccggca
    32101 gtgggattct tcgcttcggt gatgtatccc atcatcttct cgctggcact caattcggtc
    32161 tcggaacatc acgggtcctt tgcgggtata atggtaacag gtatcatcgg cggtgccatc
    32221 gggccgctga tcatcggcgg tcttggcgac gcgctcggcc tccggacggg catgttcttt
    32281 ttatatctta cgcttggcta tatcctcagt atcggcttct gggccagacc cctgatcacg
    32341 aaccgtacag ttttcaataa aaacgacttc tatgagacag cccaaagtga tcggcattga
    32401 cctcggagcg accaatatcc ggggcgcagt agtcagcgga gactcgcttt ccgacatcat
    32461 ttccgtgcgt atccacagcg agggctcggt agaagacgta ctgaacgata tttaccagct
    32521 gatcgaccgc ctcaccggcc ccgatatcgc cggtatcggc attggcgtac ccagcgtagt
    32581 ggatgtggag gagggcatag tctatgatgt gcagtatatc ccttcctggg tggaggtacc
    32641 cctgaagcaa ttgctcgggg agcgataccg gctgcccgta tacgtcaata acgacgctaa
    32701 ctgcttcgcg ctgggtgaat actatttcgg aaaaggaaag ggcatgtctt cgctggtcgg
    32761 actgaccatc gggacaggac tcggcggcgg ggtcgtcatc catgacaaat tgtatcccgg
    32821 atataactgc ggggcaggcg agctcgggct cttgccctac agggacaata tcctcgaata
    32881 ttattgcagc gggtcctttt ttaaaaattt acacggactg gacggcgtaa tggtctacga
    32941 ggcggcgcgc gagggcgatg ccagggcgct ggagctgtac gcggagctgg gagaacatat
    33001 aggccagggc atcaaggcgg tcgtatacag ctatgacccg caactgatcg tcctgggcgg
    33061 atcggtcagc gcagcctacg acctgttcca gcagacgatg tgggagacca tccggtcact
    33121 ggcatatgcc aaaacccttg cacggctgaa gatcgaagtt tctaacctga aaaatagcgg
    33181 catcctcggg gctgccgcac tatattatga ttctacataa tcactacact caaattattg
    33241 ccgtcctggc cttctcgttc gcgatgacgc cgatgacgcc agcctctgcc caatctcccg
    33301 gacactacag tatggtcctg aaggaaggct gggaaatgca gtccgtcaca aaagtgccgg
    33361 atgacggggc cactatttcc caaaaaaagt atacccctga gggttggtac aaggtggagg
    33421 tgccgacgac gatcatcgcg gggctgctgg ccaacaaagt ctatgatttc gatccctttt
    33481 acggtatgaa tctggaaaag ctgaaagatt cgagccttaa caggccctgg tggttccgga
    33541 aggaattcac cttgccggcc acacaaaaag gtaaggatgt catactcacc ctccatggca
    33601 tcaactataa ggcaaatgtg tggctgaacg gcgtgctcgt cgccgattcc ggccggatca
    33661 tgaaccctta ccggatcatc gcgctgaatg tcactcctta tataaaagca ggaggcgcaa
    33721 atgtacttgc actggagatc cggcgaccca tcaacccgca acggcgtggt ggcgatctgg
    33781 ccatcgacta tgccgactgg atccactacc cgccggacta taatggcggt atcgtcaacg
    33841 atatcgtgat aaagacattc gaccatgtcg ggatagagta tccgctggtg accacgcatt
    33901 tcgatctgcc gtcgctggat gtcgctcacc tgacggtgga tgcgatggtc ggcaattatt
    33961 cgtccggccc gcaggatgta gtggtgaaag gcaggatcga ctcatccatc acctttcaaa
    34021 agaaagtaca cctcgaagcg aatgaattcg ccgatgtcac ttttacgccg gcggacttcc
    34081 cccaactcaa cgtcaaacat ccgcgtatct ggtggccatg gcaatacggc aaagccgagc
    34141 tcaaccggat agagttgtcc gtgatgcgcg gcggtaccgt cagcaatagc ctttcggagg
    34201 attttggcat ccgcgagatc acttctctgt tcctcgatac cgccgcccgc gtctttatcg
    34261 tcaacggcaa acgcatcctg atccgcggcg cggcctggtc gccggatatc tttcaacgtc
    34321 gtagcccccg cagacaggag caggagatcc ggctgtacag ggatatgaac atgaatctta
    34381 taaggtcgga gggcaaactg gaagacgaca atttctacgc cctctgtgat aaatacggca
    34441 tgctggtgat gaccggctgg atgtgctgcg gcgcctggca gtacccggaa ttgtgggatg
    34501 cggccaaacg ggcttcggcc atggcgtcgg acagcagcgt gatgctctgg ctgcgcaaca
    34561 aagccagcgt gatggtttgg ctgaatggca gcgatatgcc gccgcgggac accagcgtgg
    34621 agcgggaata tttggccatc gaatcggcgt tgaaatggcc caatccgacg atctccacgg
    34681 cggatgccag caaatccaaa gtttcgggat acagcggcgt aaaaatggac gggccatatg
    34741 aatgggtgcc tcccatctat tgggagaccg attcgacgcg gcggcatggc ggcgcctgga
    34801 gctatgccac ggagatctcg ccgggaccct cgatcccgcc atatgaaagc ctgatcaaat
    34861 tcattcctaa agactcgctc tggtttacga cggccacttg gaaataccat tgcggcacga
    34921 tgaatttcgg gacgacggat gtcttcaata ccgccctgca ggcacgttat ggggaagcaa
    34981 aagacatcag ggactatgtc gacaaagcgc aggcgcagaa ctatgaggcg caccgggcga
    35041 tgatggaggc atatgggctg gataaatatc acgacgcgac aggtgtggtg caatggatgg
    35101 gcagcaaccc ctggccggga ctgatctggc atacctatga ctactatcta tacccggcag
    35161 gcacctattt cggtatgaaa aaggccctcg agccactgca catccaatat tcctatgccc
    35221 ggaatgccat agcggtcacg aactcattgc tgcaccgctt cacgaacctc cgggccgacg
    35281 caaagatcta cgacctcgat ggcagcgtca aatggtcgaa ggagcgacag ctttccgtcg
    35341 aacctgacag cgtctggctt ggactcgacg tgccggcgat cgcaggactg tccccgacat
    35401 attttctgcg cctggaatta aaggatgaga taggcaaggt gaaagatatc aactggtatt
    35461 ggttgtcggc caggggcgat gagctcgact ggaagacatc caaatggttc gtgactcccg
    35521 aaaaggcata tgccg
//
LOCUS       DASYSV010000354         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_326098, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000354 DASYSV010000000
VERSION     DASYSV010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326098"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="VGQ51_10820"
     CDS             <1..1340
                     /locus_tag="VGQ51_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEV9037107.1"
                     /translation="YNLTGEKFLLDLAELIHHQVFDWTGTFLHSEDVRQTYRFHGVNL
                     AQGIKEPVIYYQQHPEEKYIRAVKDAFRDIRNYQEQVQGMYGADEMTRGNDPAQGSEL
                     CSAVEMMYSLENMIAITGDVAMMDHLERIAYNALPAQISADYSSRQYYQQANQVMVTR
                     HQRNFVTSYDGTDLCFGVLTGFPCCTTNMHQGWPKFVQNMWFATEDKGIAALLYGPSE
                     VTARVASGDEVTITEQTQYPFEGKVAFKMKTGKEVEFPFHLRIPGWCDSATIRVNGQV
                     ERVAKGNQVIRINRRWRDHDVVELFFPMKNRVSRWFNNSVGIERGPLVYGLRIGEQWK
                     EVKNGDHYGDYKEVLPTTPWNYGLTEETVLDPDSIEVKTKKFIGDPWTLENAPVELKV
                     RGKRIPEWTLYNETAGPLPYSPIEYLKKEDPVTLTLVPYGCTKLRISEFPVVK"
     gene            1379..>3577
                     /locus_tag="VGQ51_10825"
     CDS             1379..>3577
                     /locus_tag="VGQ51_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9037108.1"
                     /translation="MNYKRCLRVIFFGLLGVYWTGLHAQTTRIHGKILNQHDRSVING
                     ATVRVKNGTAYTFSDQQGNFELSYEFKRGDSLEITSVGYAPQMVAISVGQPVTVLMSV
                     ESRALTDVVVTALGIKKEKKRLGYAVQEVKGEALVKAREPNVLAGLTGKVAGLRIANS
                     ADFFGTPDIKLRGVTPLIVVDGVPLQGTTMWEISADDVDNYSVLKGPAASALYGWRGV
                     NGAIQITTKKGSRTRGPEFSLNSTTTFDLGTIQEPRLQNEYGTGYDGIYKQGDPTYEY
                     WGAWGPRLDGRMLVQYNSPVNPDGTKEPLPWIARGAKNLQHFLRTGVLTTDNISVSAG
                     NENGNFRASLTQMYQKGVVPNTRLGGTTFNMSGGLKLNQRLSLDADINYNKFYTPNYP
                     SIGYGRASFIYGLVLWTGANVDVRDLRNYWVPGQEGLQQMNYDDAGDYNNPYFVAYEQ
                     TNGYYKDNIFGNVSLKYNLSKNLNITARTAVNSNALYQPKVYPQSYNHIPQGNYSENW
                     YNMFESNSDVLVDYSKKAGDFEIKVNAGASARNWSERNIYGSTDGLSVPGWNNFGSSI
                     NPSTPTNYKNQLQEYGTYASADLGYRNLLFLGVTGRYDWSSTLPAEHDGYFYPSASLS
                     TILNEYIDLPKVISYAKLRAALARVGSGMTPYSFTTAYIPGVRWNDNLTLGYPATIIS
                     PNVKPSFSTGFETGGDFRLFGNRVGLDLTYFNFVDGPQTYYQAISNTSGFT"
BASE COUNT          882 a          895 c         1033 g          767 t
ORIGIN      
        1 tgtacaatct cacgggtgag aaattcctgc ttgatctggc cgagctgatc catcaccagg
       61 tgtttgactg gacgggcact tttctgcatt ccgaagacgt acgtcagacc taccgctttc
      121 atggggtgaa cctcgcgcag ggcatcaagg aaccggtgat ctattatcaa cagcaccccg
      181 aagaaaagta tatccgcgcg gtaaaagatg cctttcggga tattcgcaat taccaggagc
      241 aggtgcaagg tatgtatggc gccgacgaaa tgaccagggg aaacgacccg gcacagggct
      301 ctgagctttg ctcggccgtc gagatgatgt actcgctgga aaacatgatt gcgataaccg
      361 gcgatgtggc gatgatggat catctcgagc gaattgccta caacgctttg ccagcccaaa
      421 tatccgccga ctattcctcc cggcagtatt accagcaggc caaccaggtg atggtcaccc
      481 gtcatcaacg aaattttgtg acctcctatg acggcaccga tctgtgtttc ggcgtactca
      541 ccggtttccc ttgctgcacg accaatatgc accagggctg gccaaagttc gtccagaaca
      601 tgtggttcgc aacggaggat aagggcatag ccgccttgtt atatggccca tccgaggtga
      661 cagccagggt cgcaagcggc gacgaggtca cgatcaccga acagacgcaa tatccgttcg
      721 aagggaaggt ggcatttaaa atgaagaccg ggaaggaagt cgaatttccg ttccatctgc
      781 ggatacccgg gtggtgcgat tcggctacca tacgcgtgaa cgggcaggtc gagcgggtgg
      841 cgaaagggaa tcaggtcatc cggatcaacc gacgctggag ggaccatgat gtggttgaac
      901 tcttctttcc tatgaagaac agggtcagca gatggttcaa taattcggtc ggcatcgaac
      961 ggggcccatt ggtgtatggg ctgcggatcg gcgagcagtg gaaggaggta aagaacgggg
     1021 accactatgg cgattataag gaagtgcttc cgacaacgcc atggaattac gggttgacgg
     1081 aagagactgt actggatccg gattcgatag aggtcaagac aaagaagttc attggcgacc
     1141 cctggacgct ggaaaatgcc cccgtcgagc taaaagtgag ggggaaacgc attcccgagt
     1201 ggacattgta caatgaaaca gccggtccat tgccctacag ccccattgaa tatctaaaga
     1261 aggaagaccc tgtgacgctc acgctggtcc cctatggttg cacgaagctg cggatcagcg
     1321 aattcccggt agtgaaataa ccaagaagta taatcaataa ttaccaaacg atacacttat
     1381 gaactacaaa cgatgcttgc gggtaatttt tttcggcctg ctcggtgttt attggacagg
     1441 tttgcatgcg cagacgacga ggatacatgg aaagatcctc aatcagcatg accggtcggt
     1501 cattaatggc gcgaccgtcc gggtcaagaa tgggacggct tacacttttt cggatcaaca
     1561 gggcaatttt gagttgtcgt atgaattcaa gcggggtgat tcgctggaga tcacatcggt
     1621 gggatatgcg ccgcaaatgg tcgcgatctc cgtcggccag ccggtgaccg tcctgatgag
     1681 cgtggagtcc agggcgctga cggatgtggt cgtcacggcg ctcggtatca aaaaggaaaa
     1741 gaagcggctg ggttatgccg tccaggaggt gaaaggggaa gcgcttgtca aggcgcgtga
     1801 accgaatgtg cttgcggggc tgacgggcaa agttgcgggt ctgcgtatcg ccaattccgc
     1861 cgatttcttc ggtacgccgg acattaaatt gcggggcgtg acgccgctga tcgtggtcga
     1921 tggagtaccg ctgcaaggga cgacgatgtg ggagatctcg gcggatgacg tagacaatta
     1981 tagcgttttg aaaggacccg cggcatccgc gctgtatgga tggagaggtg tcaacggggc
     2041 gatccagatc accacgaaaa aaggcagcag gacgcgtgga ccggagttca gcctcaactc
     2101 gacgacgaca ttcgatctgg gtaccatcca ggagcccagg ctgcaaaacg aatacggaac
     2161 aggttatgac ggtatctata aacaggggga cccgacttac gaatactggg gcgcctgggg
     2221 accgaggctg gacgggagaa tgctggtcca gtacaacagc ccggttaatc ccgatggtac
     2281 gaaggagccg ctgccctgga tcgcccgggg ggcgaagaac ctgcagcatt ttctccggac
     2341 gggggtgctg accaccgata atatcagcgt ctcggcggga aatgagaatg gcaattttcg
     2401 cgcgtcgctg acgcagatgt atcagaaggg agtcgtgccg aatacccgtc tcggtggaac
     2461 caccttcaat atgtccggtg gtttgaagct gaaccaacgg cttagcctcg atgccgacat
     2521 caactacaat aagttctata cgccgaatta tccgagtatc ggatatggcc gggccagttt
     2581 catttacggg ctggtgctat ggacgggcgc caatgtggac gtgcgggatc tccgcaatta
     2641 ctgggtaccc ggtcaggaag gattgcagca aatgaactac gacgacgccg gcgactataa
     2701 caatccttat tttgtcgctt acgagcagac gaacggttac tataaggata acatcttcgg
     2761 gaatgtgtcg ctgaagtata atctctcgaa gaacctcaac attacagcca ggaccgcggt
     2821 caacagcaac gcgctatatc agccgaaagt gtatccgcag tcctacaatc acattccgca
     2881 gggcaattat tcggaaaact ggtacaatat gttcgagtcc aactccgatg tcctggtcga
     2941 ttacagtaag aaggccgggg atttcgagat caaggtgaat gcgggcgcca gtgcgaggaa
     3001 ctggtcggag cggaatattt acggatctac cgatggtctc agcgtaccgg gctggaataa
     3061 tttcgggagc tcgatcaatc cttccacccc caccaattat aagaaccagc tgcaggaata
     3121 tggtacatat gccagcgccg acctcggcta caggaatctt ctcttcctgg gtgtaacggg
     3181 tagatatgat tggtcgtcca cgctaccggc ggagcacgac ggctattttt acccttcggc
     3241 ctcgctctcg accatcctca atgagtacat cgatctgccg aaggtgatct cttatgccaa
     3301 actccgcgcg gcactggcac gagttggctc cggaatgacg ccctacagct ttacgacggc
     3361 ctatattccc ggggtgagat ggaacgataa tctgaccctc ggctatcctg ctacgatcat
     3421 cagcccgaat gtcaagccgt ctttcagcac cggcttcgag accggcggcg acttccggtt
     3481 gtttggcaac cgggttggcc tggatcttac ctatttcaat tttgtggatg gcccgcagac
     3541 ctattatcag gcgatatcca atacttccgg tttcacc
//
LOCUS       DASYSV010000355         5758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1352065, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000355 DASYSV010000000
VERSION     DASYSV010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352065"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="surE"
                     /locus_tag="VGQ51_10830"
     CDS             complement(<1..661)
                     /gene="surE"
                     /locus_tag="VGQ51_10830"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEV9037109.1"
                     /translation="MIKKGSNGRKTAATRKPGKTGATGIIKKTDKTERPVRTGKTGPT
                     GIIRETGTNGMTDMVNGVESTTGGARKPASAGIKVRGGKSQPSGNGAKGGKKRVPVIL
                     VTNDDGISAPGIRNLVEAVKGLGKVVVVAPDKPQSGMGHAITIGNPLRLTPMHHLFEG
                     VEAWSCSGTPVDCVKLAVDKVLRRKPDLCLSGINHGANHSINVIYSGTMSAAVEAAIE
                     SI"
     gene            782..1663
                     /gene="atpG"
                     /locus_tag="VGQ51_10835"
     CDS             782..1663
                     /gene="atpG"
                     /locus_tag="VGQ51_10835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEV9037110.1"
                     /translation="MPGQLKEVRNRIKSIQNTQQITKAMKMVSAAKLRRAQDAIIQMR
                     PYAQKLQELLSNIVSNSEGDLGMDLAIERPVEKVLLIVITSDRGLAGAYNANVIKLAK
                     ATIREKYSSQSAKGNVTIWSIGKKGYEHFQKNNYKVTDTYKDIFLHLDFQHVQAAAQA
                     AVKAFANKEFDVVEIVYSQFRNAATQTFVSERFLPIPKVEKKPGATKSDFIFEPGKEV
                     LITELMPKILNTQLYKAVLDANASEHGARMTAMDKASDNANEMLKVLRLSYNRARQAA
                     ITTELTEIVSGAAALQG"
     gene            1683..2549
                     /gene="scpB"
                     /locus_tag="VGQ51_10840"
     CDS             1683..2549
                     /gene="scpB"
                     /locus_tag="VGQ51_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEV9037111.1"
                     /translation="MEIGNIIPHIEALIFASDKPLTSMEITELLNNAFGFMEEKLTLE
                     QIQTSLDGIIEKYASEFYPFEVRESGGGWQFLTKKDYHKTVAQLNGEKFLKRLSSAAL
                     ETLSIIAYKQPITKGEIESIRGVNSDYSIQKLLEKELVVISGRNENMPGKPLVYGTSK
                     TFMDYFGINSSEDLPKLKEVFAETLVEPTMVPPPFADDEFSPGTGEAAAPPHPAAPTH
                     SAAPEPAPAQEPDATADAAPPHPAAPTHSAASAPAPAEADAAPTHPDPSPHPATPTHD
                     PASDESDDDPQP"
     gene            2561..3772
                     /gene="lysA"
                     /locus_tag="VGQ51_10845"
     CDS             2561..3772
                     /gene="lysA"
                     /locus_tag="VGQ51_10845"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV9037112.1"
                     /translation="MSQQPDPQYLITLGQQFGTPLYVYHAEKIKEQYLKLTTAFSDSN
                     TVFFYACKALTNINILKYVHSLGANVDCSSINEVRLALHAGFTPNRILYTSNGIGFDE
                     IAQAKELGVIINIDSLSNLEKFGQKYGHSYPVGVRLRPNIMAGGNLKISTGHDKSKFG
                     IPVDQIDKVLDLVNKYDLRIADLHIHTGSEIKDVDVFVKGIEILFDLIPKFKELQFID
                     LGGGFKVPYKDGDGETNISELAKKVKQAFENHPNPGNRPLQVWFEPGKFLVSECGYFI
                     TKVNVLKQTDAATFVSIDSGFNHLIRPMFYDAYHRIVNVSNPHGAHKRYSVVGNICET
                     DTFAWDRSISEVREGDYLVFYNAGAYGFEMSSNFNSRYKPAEVLLKDGQAHLIRRRDT
                     FEDLLRNQVEL"
     gene            3824..5449
                     /locus_tag="VGQ51_10850"
     CDS             3824..5449
                     /locus_tag="VGQ51_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEV9037113.1"
                     /translation="MPIRMVDDPRDSEENSGDNGGGGGYDSGGGGGGGGLIGLLLLLL
                     GLFRGRGLIFLLIILGGGFFLLGRPGCNPNGGTGPLHNVAQRLFTGGFLDPRQFEKAS
                     IYEGLSADSTKNPLPEAANLQRYAPAVGDQGHQGSCVAWSSAYAARTIEEAARTGADP
                     NSLRFSPSFLYNQIGIDNCDGSYIERAMEFMTNSGSIPYDQFPYNDQDCSKQPDQQLL
                     DEARQYKMRGFNRLTPGDRNNVIDLHAIKENISQGAPVVIGMMVGGSYMQPMAGQDLW
                     QPTPDDRSLLGFGGHAQCVVGYDDRKYGGAFLIMNSWGPKWGNNGFAWVRYPDFRFFV
                     REAYGIEPMARTGAAATEPFSCEIGLVQIHYEGDKTVSGGYIPLQIKTSNVFETVDPV
                     KMGTRFKMEVRNRSECYVYVFGKETDGTSYTLFPYPATGEPQKSKYSPFCGITGYRLF
                     PRDKSMMPDSIGNRDEMAVVVSRQPLDWYALNKTISANPGQDYGVRVDAALADRLLKH
                     VDFKSSAKGNMQFTVPDPRPNSVVATIVEISKK"
     gene            5454..>5758
                     /locus_tag="VGQ51_10855"
     CDS             5454..>5758
                     /locus_tag="VGQ51_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEV9037114.1"
                     /translation="MTNSAFFSAIRHPLKSRLFLLTNLPSALFSGLRVVEADTDHCAV
                     TVPYKWFTRNPFRSTYFACLSMAAEMSTGVLAMAAANESQPPVSMLVVRTEADYTKKA
                     "
BASE COUNT         1372 a         1848 c         1415 g         1123 t
ORIGIN      
        1 gaatgctttc gatggccgcc tctacggccg ccgacatggt ccccgaatag attacgttga
       61 tcgaatggtt ggccccatga ttgatcccgc tgagacagag atccggtttg cgccgcaaga
      121 ccttgtccac ggccagcttt acgcaatcca cgggcgtccc ggagcaggac caggcctcca
      181 ctccttcgaa aagatgatgc atcggcgtca gacgcagcgg attgccgatg gtaatggcat
      241 gacccatccc ggattgcggc ttgtcgggag ccacgaccac gacttttccc aggcctttga
      301 cggcctccac caggttgcgg atgccgggag cgctgatacc gtcgtcattg gtgacgagga
      361 tcaccggtac gcgctttttg ccacccttcg caccgttgcc ggagggttgg gatttcccgc
      421 ctctcacttt tatgcccgca gatgcaggct tccgtgcacc gcctgtcgta gactcaaccc
      481 cattgaccat atccgtcatg ccattcgtgc ctgtctcacg gatgataccc gtaggtcccg
      541 tctttccggt ccgcaccggc cgttccgttt tgtcagtctt cttgatgata cccgttgcgc
      601 cggtctttcc cggtttgcgc gtagcggcgg ttttacgccc gttagatcct ttctttatca
      661 tagttggact gcaaaaatcc ataatatttc gcaattttcg gccgatcgga cgcggaaatc
      721 ggtggccacg acatggtttt ttcccgtctt tgcgctattt ttgcacccat tttagaaacc
      781 tatgccagga caactcaaag aggtacgcaa tcggatcaag tccattcaga acacccaaca
      841 gatcaccaag gccatgaaga tggtcagcgc tgcgaaactt cgccgggctc aggatgccat
      901 catccagatg cgtccctatg cccagaagct gcaggagctg ctcagcaaca tcgtcagcaa
      961 ttccgagggc gatttgggga tggaccttgc cattgaacgc cccgtcgaaa aagtccttct
     1021 catcgttatt accagcgacc gcggtctggc cggtgcctac aatgccaatg ttatcaaact
     1081 ggcaaaggcc accattcgtg agaaatattc atcccagtcc gccaaaggca acgttaccat
     1141 ctggagcatc ggcaaaaagg gctatgagca tttccaaaag aataactaca aggtcaccga
     1201 tacatataag gacatcttcc ttcacctcga ttttcagcac gtgcaggccg ccgcacaggc
     1261 cgcggtaaag gccttcgcga acaaggaatt cgatgtggtg gagatcgtgt atagccagtt
     1321 ccggaacgcc gctacgcaga ccttcgtttc ggagcggttc ctcccgatac cgaaggtgga
     1381 aaagaagccc ggcgcaacca aaagcgattt tattttcgaa cccggcaaag aagtgctcat
     1441 caccgagctc atgccgaaga tcctcaatac ccaactgtat aaggccgtcc tcgacgccaa
     1501 tgcctccgag cacggcgccc ggatgaccgc catggataag gccagcgaca acgccaatga
     1561 aatgctgaag gtcctgcgcc tgtcctataa ccgcgcgcgt caggctgcca tcacgaccga
     1621 gcttactgag atcgttagcg gcgccgcagc gctccaggga taagcagaga agagtacaca
     1681 ttatggaaat cggaaacatc atcccccata tcgaagcgtt gatattcgcc agcgacaagc
     1741 cgctgacctc catggagatc accgagctgc tcaacaacgc attcggcttc atggaggaaa
     1801 agctgacgct cgaacagatc cagacctcgc tcgacggcat catcgaaaaa tatgcctccg
     1861 aattctatcc tttcgaagtc cgcgagagcg gtggaggatg gcaattcctc accaagaaag
     1921 attatcacaa gacggtagcc cagctcaacg gggaaaagtt cctcaaacgc ctgtcgtcgg
     1981 ccgcgctgga gaccctgtct atcattgctt ataagcaacc cattaccaaa ggggagatcg
     2041 aatccatccg cggcgtaaac agcgattatt cgatccagaa gctgctcgaa aaagagctgg
     2101 tcgtcatctc cggccgcaac gagaatatgc ccggcaagcc gctggtctat gggacgtcga
     2161 agaccttcat ggactatttc gggatcaatt cctccgagga tcttccgaaa ctgaaagaag
     2221 tgttcgccga gacactggtc gagccgacga tggtgccgcc tccatttgca gatgatgagt
     2281 tctcgcccgg gacaggcgaa gccgccgcac ccccgcaccc cgccgcaccc acgcactccg
     2341 ccgcacccga accagctcct gcccaagagc ccgacgcaac agccgacgcc gcacccccgc
     2401 accccgccgc acccacgcac tccgccgcat ccgcgccagc accggcggaa gccgacgccg
     2461 cacccacgca ccccgaccca tccccgcacc ccgccacacc cacgcacgac cccgcatcag
     2521 acgagtcgga cgacgacccc caaccctaaa ttccctaccc atgtcccaac agccagaccc
     2581 ccaatacctg atcacactcg gccaacaatt cggaacaccc ctgtacgtct atcacgccga
     2641 aaagatcaaa gaacaatacc tcaagctgac caccgcattc agcgacagca acaccgtctt
     2701 cttttatgcc tgcaaggccc tcaccaacat caacatctta aaatacgtcc atagcctcgg
     2761 cgccaatgtc gactgcagca gcatcaatga ggtccgcctc gcgctccatg ccggcttcac
     2821 gcccaaccgc atcctgtata cgtccaacgg catcggcttc gacgagatag cgcaagccaa
     2881 agaactcggc gtcatcatca atatcgacag cctctccaat ctcgaaaaat tcggacaaaa
     2941 atacggtcac tcctacccgg taggcgtgcg tctccgcccc aatatcatgg ccggcggtaa
     3001 cctgaagatc tccacgggtc acgacaaaag caaattcggc atccccgtcg accagatcga
     3061 caaggtgctg gacctcgtga acaaatatga tctccgcatc gccgacctcc acatccatac
     3121 cggcagcgag atcaaggacg tcgacgtctt cgtcaaaggc atcgagatcc tcttcgacct
     3181 catcccgaaa ttcaaagagc tccaattcat cgacctcggc ggcggattca aggtgccgta
     3241 taaagacggc gacggggaga ccaatatctc cgagctggcc aaaaaggtca aacaagcctt
     3301 cgaaaaccat ccgaaccccg gcaaccgacc attacaagtc tggttcgaac ccggaaaatt
     3361 cctcgtcagc gaatgcggct atttcatcac gaaggtcaat gtcctcaaac agaccgacgc
     3421 cgcgactttc gtcagcatcg acagcggatt caatcacctc atccgcccca tgttctacga
     3481 cgcatatcat cggatcgtca acgtcagcaa cccgcacggc gcgcacaaac gctattccgt
     3541 ggtcggcaat atctgcgaga ccgatacctt cgcctgggac cgcagtatca gcgaagtccg
     3601 cgagggcgat tatctcgttt tctacaacgc cggcgcctat ggattcgaga tgtcctccaa
     3661 tttcaattcg cgctacaaac ccgccgaggt tctcctcaaa gacggccagg cgcatctcat
     3721 ccgccgccgg gataccttcg aagatctgct gcgcaaccag gtagagctct aaccgcccat
     3781 cccctgcgca aaaagtttta catttaggta aaacttttat cccatgccca taagaatggt
     3841 cgacgatcca cgcgactccg aagaaaacag tggcgacaac ggcggaggcg gcggttacga
     3901 ctcgggagga ggtggcggcg gaggcggact catcggtctg ctgctcctgc tgctcgggct
     3961 cttccgcgga cgcggcctca tcttccttct gatcatcctg ggaggcggct tttttttatt
     4021 aggtcgcccc ggctgtaacc caaacggcgg aaccggaccc cttcacaacg ttgcccaacg
     4081 ccttttcacc ggcggattcc tcgatccccg tcaattcgaa aaagctagca tctacgaggg
     4141 cctctcagcc gattctacaa aaaacccgct tcccgaggct gccaatctcc agagatatgc
     4201 acccgccgtc ggagaccagg gccatcaggg aagctgtgtc gcctggagca gtgcctatgc
     4261 cgcccgtacc atcgaagagg cggcacggac gggcgccgac cccaacagcc tgcgcttcag
     4321 cccctccttt ctctataacc agatcgggat cgacaactgc gacgggtcgt atatcgaacg
     4381 cgccatggaa ttcatgacca atagcggcag cattccgtat gaccagttcc cctataacga
     4441 ccaggactgt tcgaaacaac ccgaccagca gctgctggat gaggcgcggc aatataaaat
     4501 gcgcggtttc aaccgtctca cgcccgggga tcgcaacaat gtcatcgatc tccatgccat
     4561 caaggagaat atctctcagg gcgcccccgt ggtcatcggc atgatggtgg ggggcagtta
     4621 catgcagccc atggcgggcc aggacctctg gcagcctaca cccgatgacc gaagcctcct
     4681 cggcttcggc ggccacgcac aatgcgtggt aggatatgac gacaggaaat acggcggcgc
     4741 cttcctgatc atgaacagct ggggccccaa atggggcaac aacggattcg cctgggtgcg
     4801 ctacccggat ttcagattct tcgtccggga ggcgtatggc atcgaaccca tggcacgtac
     4861 cggcgccgcc gcgaccgaac ctttctcctg cgagatcggg ctggtacaga tacattatga
     4921 aggcgacaag accgtctcag gcggctatat tcctttacag atcaaaactt ccaatgtctt
     4981 cgagaccgtc gatcccgtaa aaatgggcac gcgttttaaa atggaggtcc gcaaccgcag
     5041 cgaatgctac gtgtatgtct tcggaaagga gacggatggc accagctata ctcttttccc
     5101 atatcctgca acgggcgaac cacaaaaatc gaaatattct cccttctgcg gcatcaccgg
     5161 ctaccggctc ttcccgagag ataaaagtat gatgccggat agcatcggaa atcgtgatga
     5221 aatggccgtc gtcgtcagcc gccaaccgtt ggactggtat gcgctcaaca agaccatcag
     5281 cgcgaatcct ggtcaggatt atggcgtccg cgtcgacgcg gcccttgcag accgcctgct
     5341 gaagcatgta gacttcaaga gctcggccaa aggcaatatg cagttcacgg tcccggatcc
     5401 ccgccccaac agcgtggtgg ccaccatcgt agaaatcagt aaaaaataag atcatgacca
     5461 actccgcctt tttcagcgcc atccgccatc ctctgaaatc gcgtctcttc ttgctgacca
     5521 atctccccag cgccctcttc tcgggcctcc gcgtggtcga ggccgacacc gatcactgcg
     5581 cggtcaccgt tccctacaaa tggttcacgc gcaacccctt tcggtccacc tatttcgcct
     5641 gtctgagcat ggcggcggaa atgagcaccg gcgtcctcgc catggcggcc gcaaacgaat
     5701 cccaaccacc cgtgtccatg ctcgttgttc gcacggaggc cgactatacg aaaaaggc
//
LOCUS       DASYSV010000356         6642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_968826, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000356 DASYSV010000000
VERSION     DASYSV010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968826"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VGQ51_10860"
     CDS             complement(<1..499)
                     /locus_tag="VGQ51_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9037115.1"
                     /translation="MKWAIYSCFFFFAGHCFATDSLSVRYLGIDQGLSNNAVTCIYKD
                     HNGFMWFGTYDGLNRYDGYGFHVFRNIIGDSASLLDNHVYCINSDNENRIWIGGVKGA
                     SVYDPGRSAFFTPRFRQAAQTVPLFDDALSVKNAAGTMLVGTSHDGLIVFRDGSTTGK
                     QIALPG"
     gene            complement(496..4047)
                     /locus_tag="VGQ51_10865"
     CDS             complement(496..4047)
                     /locus_tag="VGQ51_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEV9037116.1"
                     /translation="MFVAGILSCNRTHLFERISSSHSGITFNNKIVENDSINPLSVVN
                     IYSGGGVGIGDFNNDGLPDIVLTGGMVPSKLYLNKGNFKFDDITAKAGVEGMGRWARG
                     VSVVDINNDGLLDIYVCNTLYKDPLRRRNILYINLGPDKNGIPHFKDMAAEYGLDIDV
                     QSTMASFFDYDNDGDLDMYLTVNEASGGYESSVFIGQDQPTGGANRGRLYRNDMDPRL
                     GHPVFHDVTDEAGIKFEGYGHAATICDINNDGWKDIYVSDDFISNDILYINNHDGTFS
                     NRTKEYFKHTSYNSMGQDVVDINNDGLPDVVELDMSAEDNYRKKMMASANNTNTYQNF
                     ENYGYQYQYVRNTLQLNQGPSVGQNDSIGIPAFSEVGFMSGMSQTDWSWTPLVIDFDN
                     DGYRDLVITNGFPRDWSDHDFIAYRQQTGAFTGNALPQKSGGLVSNMKLLDMIPQVKL
                     HNYAFRNDGAAGFADVSDSWGLGLPTFSNGAAYADLDNDGAMDMVINNIDDEALLYRN
                     TSRDKNKDSTHFLNIRFKGGKQNINGIGATANIFYDHGKQQVYDNNPYRGYLSSVQCM
                     GHFGLGKITRIDSVLIKWANGRQQTIKDIRADQTIDVTIADAKETHSYTPPKIDPLFT
                     EVTAEAGINYIHKDIPYIDFTIQALLPHKLSEYNPALAATDVNGDGLDDLVIGGNPAD
                     PAKLLLQQPDGKFIRKDLAPDQAKDEGILVFDANGDGKPDLYLARGGYGAAYDSVHYQ
                     DKLYINDGRGNFTEEPAALPINHTSKLCVRAFDYNNDGKLDLFVSGRVMPGGYPKPVS
                     SFIFRNASRNGRAKFTDVTTEVAPELKNIGMVCDALFTDFDNDGATDLIVVGEWMPVT
                     FFKNVNGKFRNVTNASGIAGRTGWWNSIVAGDFRHTGRMDYVIGNVGLNTYYKGNEEF
                     PAYITAKDFDNNGAFIALTSLYLPDIQGRRKEFPAFGRDDIARQYPGIKKRFATYKPF
                     ALATMDDILTPDQRRGALRLKANTFESCYLRNDGSGKFTLIPLPREAQVSVINAMVVD
                     DFDGDGNLDILMNGNDYGTEVSTGRYDALNGLLLKGDGNGKFFPQSIRESGICIPHDG
                     KALVKLVGASGDYLVAASQHQDALKLYRLKRKVKAIRIDPDEISAVVIGKDGRSRKEE
                     FYYGNSFLSQSSRFMLIDSTVRSVTIRNARGNSRILK"
     gene            complement(4064..5917)
                     /locus_tag="VGQ51_10870"
     CDS             complement(4064..5917)
                     /locus_tag="VGQ51_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 35 protein"
                     /protein_id="HEV9037117.1"
                     /translation="MNKICAILFVFAMASATRGHAQQQHSFALGKNAFLLDDKPFQMI
                     CGEMHYSRIPREAWRQRMKMARAMGLNTIGTYVFWNIQEPEKGKFDFSGNNDIAAFVR
                     TAREEGLWVVLRPSPYVCAEWEFGGYPYWLQNEKGLVVRSKEKSYLAEYRRYLLALGK
                     QLAPMQVNHGGNILMIQVENEYGSYGDNKEYLEINRRIFQEAGFDGLLYTCDQANDLA
                     KGYLPGLLPAVNGIDDPQKIKQLVRKYNNGNGPYYVAEWYPAWFDWWGTAHHIVPPEK
                     YAAVLDNLLSAGISVNMYMFHGGTTRGFMNGANYNNKNPYEPQISSYDYDAPLDEAGN
                     PTPKFMAFRSVIQKHLPPGETLPDVPAPKPVVSIDEIHFTEATSIFSILSTPRVSRDL
                     LTFEDLHQAYGFVLYRTTVNGGGKAKLVLKGLRDYALVFIDGKRTSVLDRRHNDSSSN
                     VDLPPGRVTLDILVENLGRINFGPYLLKNKKGITREVLLNGQELKDWQMFSLPFNDIN
                     TIRRTSAAGRYTQAPVIRRGSFHLDGVGDAYLDMSKWGKGIVWVNGYNLGRYWNVGPQ
                     QTLYVPAEWLKPGNNEIVVLELIKPEQDVLRGIGKPLLSQLQEKEPLGDSN"
     gene            complement(6024..>6642)
                     /locus_tag="VGQ51_10875"
     CDS             complement(6024..>6642)
                     /locus_tag="VGQ51_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9037118.1"
                     /translation="QYEVGGFDTHNPPTGANIWNWSPTGYYLRKWSDATIDPLKVLQS
                     TPWRYIRYTEVLLNYAEASLGLGDAATAKTYINMIRTRAGMPNIATATMASLQHERKI
                     ELAFEELRYFDIRRWMICDPAVYGQKAGFSNVWAIHIFYPYNGSTAYGSSTPVYTPVA
                     DVSGAGNYANLDYSGGRAWNPKYYFLPIAYDEMNKNLKLIQNPFY"
BASE COUNT         1515 a         1697 c         1685 g         1745 t
ORIGIN      
        1 ctcccggaag cgcaatttgt ttgcctgtcg tgctgccatc cctaaaaaca atgagcccat
       61 catgcgatgt gccgaccaac atcgtacccg cagcattctt taccgacaag gcgtcgtcga
      121 acaaggggac ggtttgtgcc gcctgtctga accggggcgt gaagaatgca gaccggcccg
      181 gatcataaac gctcgcaccc tttacacctc ctatccagat cctgttctca ttatcggagt
      241 tgatacaata gacatggtta tccaaaagag aggcggaatc tcctattata ttcctgaaaa
      301 catggaaccc gtaaccgtca taccggttca acccgtcata ggtcccgaac cacataaaac
      361 cgttgtgatc cttatagata caggttacgg cattgttcga aaggccctgg tcgatgccca
      421 gataccgcac ggaaaggctg tcggtagcga aacaatgccc cgcgaaaaaa aagaaacagc
      481 tatagatggc ccatttcatt ttagtattct actatttcct cttgcatttc tgatcgttac
      541 ggatcggaca gtgctgtcga tcaacataaa ccgggaggac tgggacaaaa aagagttccc
      601 gtaataaaac tcttctttac ggctcctgcc gtctttgccg atgacgaccg cgctaatctc
      661 atccgggtcg atcctgatgg ctttcacctt tcgtttcaat cgataaagtt tcaacgcgtc
      721 ctgatgctgg ctcgccgcaa ccaggtaatc gccggacgca ccgaccaatt ttaccagcgc
      781 cttaccgtcg tgcggaatgc atatcccgct ttcccggatc gattgaggga agaactttcc
      841 attcccatct ccttttaaca gcaacccgtt cagggcatcg tagcggccgg tagacacttc
      901 ggttccatag tcattcccat tcatcaggat gtccaggttg ccgtcgccgt cgaaatcgtc
      961 cacgaccatg gcgtttatga ccgacacctg ggcctcccgc ggcaagggga tcagggtgaa
     1021 tttgcccgaa ccgtcattcc gcaaatagca ggattcgaac gtgttggcct tcaggcgcag
     1081 ggctccgcgg cgttggtcgg gggtaaggat gtcgtccatg gtcgccaacg caaagggttt
     1141 gtacgtagcg aatctcttct tgataccggg gtactgtctg gcaatgtcat cacgtccgaa
     1201 agccggaaac tccttcctcc tgccctgtat gtcgggaaga tagagagagg taagcgcgat
     1261 aaaggcgccg ttattatcaa aatcctttgc tgtaatataa gcaggaaatt cctcattccc
     1321 tttataataa gtgtttaaac ctacattgcc gatcacgtaa tccatccgtc ccgtatgtct
     1381 gaagtcaccc gcgacgatgc tattccacca accggtccga ccggcgatac ccgaagcgtt
     1441 ggtgacgttc cggaatttcc cgttcacatt tttaaagaac gtcaccggca tccattcccc
     1501 gacgacaata agatccgtcg ccccgtcatt atcgaaatcg gtaaacagtg cgtcgcagac
     1561 cattcctatg ttcttcaatt cgggcgctac ctccgtggtg acatcggtga attttgcacg
     1621 accattccgg gaggcgttgc ggaaaataaa actgctgaca ggctttggat acccgccggg
     1681 catgacgcgg ccggatacga acagatccaa tttgccgtcg ttgttatagt cgaacgccct
     1741 tacgcagagt ttgctggtgt gattgatggg caaggcggcc ggctcttcgg tgaaatttcc
     1801 ccggccgtcg ttgatataca atttatcctg ataatgaacg ctgtcgtagg cggctccata
     1861 accgcctctt gctaaataca aatcgggttt cccatcgccg ttggcatcga atacgaggat
     1921 tccttcatct ttagcctgat caggggccag gtccttccga atgaattttc cgtcgggttg
     1981 ctgcaataaa agttttgccg gatcggcagg gttaccgccg atgacgaggt catcgagacc
     2041 gtcgccgttg acatccgtgg ctgcgagagc agggttatac tccgatagtt tgtgcggcaa
     2101 caatgcctgg atagtgaaat cgatatacgg gatgtctttg tggatatagt tgatcccggc
     2161 ttcggcggtc acttcggtga acaggggatc gatctttggc ggggtgtagg agtgtgtttc
     2221 ttttgcgtcg gcgatggtga cgtcgatagt ttgatccgcc cgtatgtcct ttatcgtttg
     2281 ttggcgaccg tttgcccatt tgatgaggac cgaatcaatg cgggtaattt tccccaatcc
     2341 gaaatgtccc atgcattgga cggacgacag atacccgcgg taggggttat tatcatacac
     2401 ctgttgtttg ccatgatcgt aaaagatatt ggcggtggct ccgatgccat ttatattctg
     2461 ttttccgcct ttgaaccgga tattcaaaaa atgtgtggaa tccttgttct tatccctgga
     2521 ggtgttgcgg tatagcaatg cctcatcgtc gatattgttg atgaccatat ccatggcgcc
     2581 gtcgttgtcg agatcggcgt aggctgcccc gttggaaaag gtgggcaacc cgagacccca
     2641 gctatcggag acgtcggcga agcccgcggc gccgtcattt cgaaaagcat agttgtgcaa
     2701 ttttacctgg gggatcatgt cgagtagctt catattgctt acgagaccgc cggatttttg
     2761 gggaagcgcg ttgccggtga aggcgccggt ctgttgccgg taggcgataa aatcgtgatc
     2821 ggaccagtct ctgggaaagc cgttggtgat gaccaggtcg cggtaaccgt cattgtcaaa
     2881 gtcgatcacc agcggagtcc agctccagtc cgtctgggac atcccgctca taaagccgac
     2941 ctcactgaag gcgggaatgc cgatggagtc attctgccct accgagggcc cctggttcag
     3001 ttgcagggta ttccggacat attgatactg ataaccgtaa ttttcgaaat tctgataggt
     3061 gttggtgttg ttggcactgg ccatcatttt tttccgataa ttatcctccg cgctcatgtc
     3121 gagctctact acgtcaggaa ggccgtcgtt gttgatgtct accacgtcct gtcccatgct
     3181 gttataggac gtatgtttga aatattcttt cgtcctgttg gaaaaggtgc cgtcatggtt
     3241 attgatgtaa agaatatcgt tcgaaatgaa atcgtcggat acatagatat ccttccatcc
     3301 atcgttattg atatcgcaga tggttgcggc gtgtccgtaa ccttcgaatt tgatcccggc
     3361 ctcgtcagta acgtcatgaa aaacagggtg gccaagacgg ggatccatat catttctata
     3421 aagtctccca cggttggctc ctcctgtcgg ctggtcctgc ccgataaaaa cggaagactc
     3481 gtacccgccg gatgcttcat taacggtcag gtacatgtcg agatcgccat cattatcata
     3541 gtcgaaaaag ctggccatgg tcgattggac atcgatatcg aggccgtatt cggcggccat
     3601 gtctttaaaa tggggaatcc cgttcttatc gggtccgaga ttgatgtaga ggatatttct
     3661 tctcctgagc gggtctttat agagcgtatt gcaaacatag atatccaata acccgtcgtt
     3721 gttgatatcg actacggaga cgcctcttgc ccatcgtccc attccctcta ctccggcctt
     3781 tgccgtaatg tcatcgaatt tgaaattgcc cttgttgaga tacagtttgc tggggaccat
     3841 cccgcccgtc agcacgatgt ccggcaggcc atcgttgttg aaatccccga tgccgacacc
     3901 gccgccgcta tagatattga cgacagacag cgggttgata ctatcgttct cgacgatctt
     3961 attattaaaa gtaatcccgg aatgtgagga ggatatcctt tcgaacagat gggtccggtt
     4021 gcaggacagg atgccggcaa cgaacaaaac gaatgtgatt gatctaattt gaatcaccca
     4081 aaggttcttt ttcctgcaat tggctaagca acggctttcc aatgcccctt agaacgtcct
     4141 gttccggctt tatcaattcc agcacgacga tctcattatt gcccggcttc agccattcag
     4201 ccggaacata tagtgtctgt tgagggccga cattccaata cctgccaaga ttatatccgt
     4261 tgacccaaac aatgcctttg ccccatttac tcatgtcaag ataagcatcg cctaccccgt
     4321 cgagatggaa ggagcctctg cgaataacgg gggcctgtgt atacctgccg gcggcggaag
     4381 ttcggcggat ggtattgata tcgttgaacg gcagtgaaaa catttgccag tcctttagct
     4441 cctgaccgtt aagcagtact tccctggtaa tgcctttttt atttttcaaa agataaggac
     4501 caaaatttat ccggccaaga ttttccacca gaatatcgag cgtgaccctg ccggggggca
     4561 gatctacgtt actgctgctg tcgttgtgcc gtctgtcgag gacggatgtc cgcttcccgt
     4621 ctatgaatac caaagcataa tcgcgtaggc ctttcaatac caatttggcc ttcccccctc
     4681 cgttcaccgt cgtcctgtac aaaacaaacc cataggcctg atggaggtct tcaaaggtga
     4741 gaaggtcgcg gcttacccga ggcgtcgaaa ggatgctgaa gatgctggtc gcttcggtga
     4801 aatggatctc atcgatggat acgacgggtt tgggggcggg aacgtcgggc agcgtctctc
     4861 ccgggggcag atgtttttgg atcacggaac ggaaagccat gaatttcggg gtcgggttac
     4921 ctgcctcatc caacggagca tcgtagtcat aactgctgat ctgcggttca taggggttct
     4981 tattgttgta attggccccg ttcataaagc ccctcgtagt accgccatgg aacatgtaca
     5041 tgtttacgga aatgccggca gagagcaggt tgtccagcac agcagcgtat ttttcggggg
     5101 gaacgatgtg atgggccgtg ccccaccagt caaaccaggc aggataccat tcagcgacat
     5161 aataagggcc attgccattg ttgtattttc taaccagctg tttaatcttt tgcgggtcgt
     5221 ctatgccatt tacggccgga agcaatccgg gtaaatagcc ttttgccagg tcattagcct
     5281 gatcgcaggt atagagcagg ccatcgaagc cagcttcctg gaatattctc cggtttattt
     5341 ccaaatattc cttgttgtcg ccgtaggagc cgtattcatt ctctacctgg atcatcagga
     5401 tgttcccgcc gtggttgacc tgcatcggtg cgagctgttt acccaacgcc agcagataac
     5461 gtctgtattc ggccaggtag ctcttttcct tgctgcgaac gacaaggcct ttttcgtttt
     5521 ggagccagta aggatatccg ccaaattccc attcggcgca aacatacggt gagggcctca
     5581 gcactaccca caggccttct tcccgggcgg ttcgcacgaa tgcggcgatg tcgttattcc
     5641 ctgaaaagtc gaatttcccc ttttccggct cctgtatatt ccaaaacaca taggtgccga
     5701 tcgtattcaa gcccatcgcc ctggccattt tcattcgctg acgccatgcc tcccgcggga
     5761 tacgcgaata gtgcatttcc ccgcagatca tctggaaagg tttgtcatcg aggagaaaag
     5821 cgttctttcc cagggcgaaa ctgtgttgct gctgtgcatg gccacgggtg gcggacgcca
     5881 tagcaaaaac aaaaagtata gcacaaatct tgttcatact tagcggattt tcatcatccc
     5941 gaaaaatgcg ggtggaatag ctgatcggta aaataaaggc cccctgtaga tacagcgagc
     6001 cttgcttacg attgcttgtg tacttagtaa aacgggtttt gaatcaattt tagattttta
     6061 ttcatttcat cgtaggcgat cggcaggaaa taatatttgg ggttccatgc cctgccgccg
     6121 gaatagtcca ggttggcata attgcctgct ccgctgacgt ccgcaacggg tgtgtacaca
     6181 ggtgtactcg atccgtaggc tgtagagcca ttgtagggat agaagatatg aatagcccat
     6241 acgttgctga agccggcttt ttgtccatat actgccggat cgcatatcat ccaccggcgg
     6301 atatcgaaat agcgtaattc ttcgaacgcc aattctattt tccgctcgtg ctgcagactg
     6361 gccatggtag cggtggcgat attcggcatg cctgcccttg tcctgatcat gttgatatag
     6421 gtctttgcgg tggcggcatc gcccaggccg aggctggctt cggcatagtt gagcaatact
     6481 tcggtatacc ggatataccg ccaaggcgtc gactggagta cctttagcgg atcgatcgta
     6541 gcatcgctcc attttctgag atagtaacca gtgggagacc agttccatat atttgcgccc
     6601 gtcgggggat tgtgcgtatc gaagccgcct acttcatatt ga
//
LOCUS       DASYSV010000357         7069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_453507, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000357 DASYSV010000000
VERSION     DASYSV010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453507"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VGQ51_10880"
     CDS             complement(<1..381)
                     /locus_tag="VGQ51_10880"
                     /inference="COORDINATES: protein motif:HMM:NF042765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6702 family protein"
                     /protein_id="HEV9037119.1"
                     /translation="MAGYLFKWLSVAALSLGMAKPHPLYISVTEISQNPKDKILEISC
                     KVFTNDFEAVLEKMSGTRVDLSAAPDKVAASRLIEQYVEKYLQLKVDGKAVALHFVGS
                     ENENDGTWSYFQVNDVAAVRRIDVV"
     gene            430..1017
                     /locus_tag="VGQ51_10885"
     CDS             430..1017
                     /locus_tag="VGQ51_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEV9037120.1"
                     /translation="MQDFQLYFGIGTHHILTWEAMDHILFVAALCLRYLIKDWKKVVV
                     LVTAFTIGHSTSLTLCALGLVHFATRWIEFLIPLTIVATAVNNLLQRPAQFEHPSKLP
                     LIYFFALFFGLIHGLAFANSLLSLIGGDHVLVPLLAFNLGVEAGQLLVVAIILAISFI
                     FVQLMKIRRTWWVWGVSTLVLVASVKMAFDRWPHS"
     gene            1046..3457
                     /locus_tag="VGQ51_10890"
     CDS             1046..3457
                     /locus_tag="VGQ51_10890"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV9037121.1"
                     /translation="MKTRLLILLSCLITTSLLAQDVKNNPNSNHGNKFEALGTILPTP
                     NEYRTASGAPGPKYWQQRCDYDIKANLDEDKLLLTGSETITYFNNSPNELTYLWLQLD
                     ENQHSSKNNADYQDGGRMQPVVSDQQLDRLAMASEPNDFGDHILKMTDALGKPLHYMI
                     NKTMMRVELPTSLKPGQKFIFNIDWNYRITDRLKVGGRGGYEFFPEDGNYLFTMTQWY
                     PRLCVYSDFQGWQNNQFTGRGEFALTFGNFKVQMTVPADHIIGATGQCQNYQQVLTPA
                     QYARWTQAQSSKEPIEIVTLAEAKASEIRKSKAKKTWIFKADNVRDFAWGSSRKFVMD
                     AMPAYVEGKKVMCMSYYGKEAYNLYRRFSTKAVAHTIKTYSHFTIPFPYPVAQSVEAS
                     NGMEYPMICFNFGRTEKDGSYSEATKYGMLGVIIHEVGHNFFPMIINSDERQWTWMDE
                     GLNSFVEYLTEELWDNHFPVRGKGPAWAIVDYMKLPKDQLEPIMTNSENIVNFGPNAY
                     SKPATALNILRETVMGRELFDYAFKEYARRWAFKHPTPADLFRTMNDASAENLDWFWR
                     GWFYGIEPVDISLDSVKHLTPDFTNVPRHKDSTLVKNIDKPMPDGRFPTDVSKERNRD
                     DKNIAFADEKDTSLHDFYYKYNRGQIPYDTSTYTTKTTFMGRREVPNFSDEPLDDSTK
                     EMLKHKQFYQLQFSNKGGLVMPIIIEWTYKDGTKEIERIPAQVWRKDESHLTKVFVKD
                     KEVASIRLDPNRETADIDESNNSWPVVQTESKFEIYKAKQQGLRAAPQANNPMQMTGK
                     DKKAF"
     gene            complement(3486..4196)
                     /locus_tag="VGQ51_10895"
     CDS             complement(3486..4196)
                     /locus_tag="VGQ51_10895"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEV9037122.1"
                     /translation="MIPGSIWLTKLLLSHLLTDFVLQPYAWVEERNRKHFASGKLYLH
                     ALLTAAVATILIGWQYWMVAIILLVTHFIIDGWKSYRPQRAEYFLIDQLLHLLVIAGC
                     WMLTFRDGDVFGRIVRWMNADTHNWIVLTGFVFVTSPAGILIGQLTERWSLRIEDPGN
                     SLVNAGKWIGIAERVIVLILVLVNQYSAIALLVTAKGIIRFSEKDRQEIKTEYLVIGT
                     LLSIGIAIVTGMVVKMMV"
     gene            complement(4193..4831)
                     /locus_tag="VGQ51_10900"
     CDS             complement(4193..4831)
                     /locus_tag="VGQ51_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037123.1"
                     /translation="MNISKATIAAVLTGDIVNSTKLLSEQEEVLFRRLRQDVLKAYPH
                     EIYRGDSFQALVEKPQEALAVALGCRALAIQFSETAVMDFDMRMSIGIGKVSLPISDL
                     GTAKGDAFLLSGRLFDTLKETGRRLIIGAGDRKADIGFEIMSDYLDSIFGKMTAKQAE
                     VIVELLNGNAQQQLSTTLNKSKSTISQLAADGGWSEIDRLLQQYNDLIAQLS"
     gene            complement(4977..5639)
                     /locus_tag="VGQ51_10905"
     CDS             complement(4977..5639)
                     /locus_tag="VGQ51_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037124.1"
                     /translation="MKRMYSAMIVVGFLLIGSRGYSQWKSYMLSPNGDTLNCVDKKDH
                     RQGRWVNHVDQLRGEAGYDEQGVYVDGRKEGLWKIFSLQGDLIGVETYKWGLKDGVCQ
                     YFGMNGSLLREEGWKALNPDKLYDTIAVEDPDHPDHYSTVIIKNEGAAIKNGTWKFYD
                     PMTGMVARTESYTLGKLEGGSNPSATAAAPPKTVSKPKEVLEFEKKAGKKKVKVQDGS
                     AY"
     gene            5722..6363
                     /locus_tag="VGQ51_10910"
     CDS             5722..6363
                     /locus_tag="VGQ51_10910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV9037125.1"
                     /translation="MKICMGFCLLLLIAAGLRAQTPPSIAQMKADLEKSPNPVLYAKQ
                     VLKKRFRIDTIVVSRTHNFDGLADSLAYYGKEKKVYGPFGPRNNRFLVQVLSKAPNQF
                     YHVGQIFIDTSVFRYRVADSLGTMILEKLRSGAASFGQMAEAYSMGGEAATKGDLGWV
                     ARGVLQPTFEHEMLFHKKGEVFKVWSRRGLHIIKKIDDPKQDTGFTLMLQVFL"
     gene            complement(6444..6863)
                     /gene="aroQ"
                     /locus_tag="VGQ51_10915"
     CDS             complement(6444..6863)
                     /gene="aroQ"
                     /locus_tag="VGQ51_10915"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEV9037126.1"
                     /translation="MQITIINGPNLNLLGRREPEVYGSQTFEQYFTELQRQFPEVSFR
                     YFQSNVEGELIDELQRSGFSSDGIILNPGGYTHTSVAIGDAIAAIKAPVVEVHISNVH
                     AREEFRRLSHVSAKAKGTIAGLGLKGYELAVRYLLRG"
     gene            complement(6854..>7069)
                     /locus_tag="VGQ51_10920"
     CDS             complement(6854..>7069)
                     /locus_tag="VGQ51_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEV9037127.1"
                     /translation="QNHGFGVDPEAVKKAKNVEITHVNLNDSSIEGIRLKDRPAFSVQ
                     YHPESTPGPHDSRYLFDEFIEMIRTCK"
BASE COUNT         1749 a         2170 c         1577 g         1573 t
ORIGIN      
        1 caccacatcg atccgccgca ccgccgccac atcattcacc tggaaatagc tccacgtacc
       61 gtcattttcg ttctcgctgc ccacaaaatg caacgcgacg gccttcccgt cgaccttcag
      121 ctgcagatat ttctccacat attgctcgat cagcctgctg gccgcgacct tgtccggggc
      181 ggccgaaaga tcgacccgcg tccccgacat cttttccaac accgcctcga aatcattcgt
      241 aaagacctta cagctgatct ccaatatctt gtctttgggg ttctgactga tctcggtgac
      301 gctgatatac aacggatgcg gcttcgccat cccaaggctt aaggccgcca cactcaacca
      361 cttaaacaag tatcctgcca tttaaaaagt ttatttcgct actacgaaag tctgaataat
      421 tttacaaata tgcaggactt ccagctctat ttcgggatcg ggacccacca tatcctgacg
      481 tgggaggcga tggaccacat ccttttcgtg gctgccctgt gtctccgcta cctgatcaaa
      541 gactggaaaa aggtggtggt cctggtgacc gccttcacca taggacactc cacatccctg
      601 accctttgcg cccttggcct ggtccatttc gctacccgct ggatcgaatt tctgataccg
      661 ttgaccatcg tagcgactgc ggtgaacaac ctgctgcaac ggcccgctca attcgaacac
      721 ccttcaaaac taccgctgat ctattttttc gcgcttttct tcgggctcat ccatggtctg
      781 gcattcgcca actcactgct gagcctcatc ggcggagatc acgtgctcgt acccctgctg
      841 gcctttaatc tcggcgtcga ggccggccag ctcctggtag ttgccatcat cctggcaatt
      901 tcctttattt tcgtacagct gatgaagatt cgccggacct ggtgggtttg gggtgtctcc
      961 acgctcgtac tggtggcatc cgtgaaaatg gcattcgatc gctggcccca ttcttaatta
     1021 gattcttttt tctaaatata cacacatgaa gacacgactg cttattctgc tcagttgcct
     1081 gatcacaaca tcactcctgg cgcaggacgt caaaaacaac cccaactcca accacgggaa
     1141 taagttcgaa gcgctgggga ccatccttcc cacacccaac gaataccgga cggccagcgg
     1201 agcccccggc ccgaaatact ggcagcagcg atgcgactac gacatcaagg ccaacctcga
     1261 cgaggacaag ctgctcctca ccgggtccga gaccatcacg tatttcaaca attcccccaa
     1321 cgagcttacg tatctctggc tgcagctgga cgaaaaccag cacagctcga agaacaacgc
     1381 cgactaccag gacggcggca ggatgcaacc ggtggtcagc gaccagcaac tcgaccgcct
     1441 ggccatggct tcggaaccca acgatttcgg agaccatatc ctgaagatga cggatgcgct
     1501 gggtaagccg ctccattata tgatcaacaa gaccatgatg cgggtcgagc ttcccacatc
     1561 cctcaaaccc ggtcagaaat tcatcttcaa catcgactgg aactaccgga tcaccgatcg
     1621 cctcaaagta ggcggtcgcg gcggttatga atttttcccc gaagacggca actacctgtt
     1681 tacgatgacc caatggtatc cgcgtctttg cgtatacagc gatttccagg gttggcagaa
     1741 caaccagttc accggtcgtg gggaatttgc cctgacgttc ggcaatttca aggtgcagat
     1801 gaccgtcccc gccgaccaca tcatcggcgc cacgggccag tgccagaact atcagcaggt
     1861 gctgacaccc gcccaatacg cccgctggac gcaagcccag tcgtcaaagg aacccatcga
     1921 gatcgtcacc ctcgccgaag ccaaagcctc cgagatccgc aagagcaagg ctaaaaaaac
     1981 ctggatattc aaagccgaca atgtccgcga tttcgcctgg ggttcgtccc gcaaattcgt
     2041 catggacgcc atgcctgcct acgtggaagg caagaaggta atgtgcatga gctactacgg
     2101 aaaagaagcc tacaatctct atcgccgctt cagcaccaag gcggtcgcgc ataccatcaa
     2161 gacttattcc catttcacca ttcccttccc ctacccggtc gcgcaaagcg tcgaggcctc
     2221 caacggtatg gaatacccga tgatctgctt caacttcggc cgcaccgaaa aggacggcag
     2281 ctatagcgaa gccaccaaat atgggatgct gggcgtcatc atccacgagg taggtcacaa
     2341 tttcttcccg atgatcatca acagcgacga gcgtcagtgg acctggatgg acgaaggtct
     2401 caactccttc gtcgaatacc ttaccgaaga gctgtgggat aaccatttcc ccgttcgggg
     2461 taagggtccg gcctgggcca tcgtggatta tatgaagctg ccgaaggacc agctcgaacc
     2521 catcatgacc aattccgaga atatcgtcaa tttcggcccc aacgcctatt cgaaacccgc
     2581 caccgcgctc aacatcctgc gcgagaccgt catgggacgc gagctcttcg attatgcctt
     2641 caaggaatat gcacggcgct gggccttcaa acacccgacc ccggccgacc tcttccgcac
     2701 catgaatgac gccagcgccg aaaatctcga ttggttctgg cgtggctggt tctatggcat
     2761 cgaacccgtc gacatctcgc tcgacagcgt caaacacctg acgcccgact tcaccaatgt
     2821 tccccgccac aaagattcga cgctggtgaa aaacatcgac aagcccatgc ccgatggcag
     2881 attccccacc gacgtttcca aagaacgcaa ccgcgatgac aagaacatcg ccttcgccga
     2941 cgaaaaagac acttccttgc acgatttcta ttacaaatac aatcgcggcc agatacccta
     3001 cgatacctcc acctatacga caaaaactac cttcatggga agaagggaag tccccaattt
     3061 cagcgacgag cccctcgacg acagtaccaa agaaatgctg aaacacaaac aattctatca
     3121 actgcagttc agcaacaaag gcggcctcgt gatgcccatc atcatcgaat ggacttataa
     3181 ggatggtaca aaggagatcg agcgtatccc cgcacaggtc tggcggaaag acgaatcaca
     3241 tctcaccaag gtattcgtga aagacaaaga agtcgcgagt atccggctgg accccaaccg
     3301 cgaaacggcc gacatcgacg agagcaacaa cagctggccc gtcgtccaga ccgaaagcaa
     3361 attcgagatc tacaaagcca aacaacaagg cctccgcgcc gcaccgcagg ccaacaaccc
     3421 gatgcaaatg acgggtaaag acaaaaaagc cttctaaata aaaaagcccc ccccgggggc
     3481 tttttttata ccatcatttt caccaccatc cccgtaacga tcgcaatacc gatactcaac
     3541 aatgtcccga tcacgagata ctccgttttt atctcctgcc ggtctttctc gctaaatcgg
     3601 ataataccct tggccgtcac cagcaatgcg atcgccgaat attgattcac caacaccagt
     3661 atcagcacaa tgacccgttc agcgatcccg atccatttcc ccgcattgac caggctgttc
     3721 cccgggtcct cgatcctgag actccaccgc tcggtcagct gaccgatcag aatacccgcc
     3781 ggactcgtca caaaaacgaa accggtcagc acgatccaat tgtgggtatc cgcattcatc
     3841 caccgcacga tcctgccgaa aacatcccca tcccggaagg tcagcatcca gcaacccgca
     3901 atgaccagca gatgtagcag ctgatcgatc aggaaatatt ccgccctttg aggccggtag
     3961 gatttccagc catcgatgat aaaatgagtg acgagaagga tgatcgctac catccagtat
     4021 tgccagccga taagaatagt tgccacagcg gccgtcagca gcgcgtgcag ataaagttta
     4081 ccggaggcaa aatgtttccg gttgcgctcc tctacccatg cgtaaggttg taggacaaaa
     4141 tccgtcaata aatgtgataa aagcagcttc gtcagccaaa tggatccggg tatcatgata
     4201 attgcgcgat taagtcattg tattgttgca ggagcctgtc tatctcggac catcctccgt
     4261 cagccgccag ctgggagatg gtactcttcg atttgttcaa tgtcgtggac agctgctgtt
     4321 gcgcattccc attcagcaat tcgacgataa cctccgcctg tttggcggtc attttcccaa
     4381 atatgctgtc caggtagtcg ctcatgatct cgaacccgat atccgccttc cggtcgccgg
     4441 cgccgatgat cagccttcgc cccgtctcct tcagcgtatc gaacagccgg ccggatagaa
     4501 gaaaggcgtc ccccttcgcc gtccccagat cggagatggg taacgacacc tttccgatac
     4561 cgatactcat ccgcatatcg aaatccatta ccgccgtttc tgaaaactgg atggcaagcg
     4621 cccggcaacc caatgccacg gcaagcgcct cctgcggttt ttctaccaac gcctggaaac
     4681 tgtctccccg ataaatttca tgtggatatg ccttcagaac gtcttgtctc agccgcctga
     4741 agagtacttc ttcctgctcg gaaagaagct tggtggaatt cacgatatcg ccggtcagta
     4801 cggcggcaat agtggctttg gaaatattca ttccgttcca agatcggtat ttttaccgaa
     4861 ctttccaaaa tttcggcaat tctaccgaac taaccaaaat ttcggcaatt ctaccgaact
     4921 aacccaaaat tcggcaattc taccgaactt acccaaattt cggcaattct accgaactaa
     4981 tacgcactcc catcctgcac cttcaccttc tttttcccag cctttttttc aaattccagc
     5041 acttctttgg gtttggaaac cgtcttcgga ggtgccgcgg cagtcgcgga aggattgctg
     5101 cccccctcca gtttacccag cgtataagac tcggtccgcg ctaccatccc ggtcatggga
     5161 tcatagaatt tccaggttcc gttcttgata gcggcccctt cgtttttaat gatgaccgta
     5221 ctgtaatgat cgggatgatc cgggtcttcc acggcgatgg tatcatatag tttgtccggg
     5281 ttcagcgcct tccagccttc ttcccgaagc aatgacccat tcattccgaa atactggcag
     5341 acaccgtctt taagtcccca tttgtacgtc tccaccccga tcagatcgcc ctgcagacta
     5401 aagatcttcc agagaccttc tttccggccg tcgacataaa cgccttgttc atcatatccc
     5461 gcctccccac gcagctgatc gacatggttt acccaacgtc cctgccgatg gtcctttttg
     5521 tctacacaat tcaacgtatc cccattgggg ctaagcatat agctcttcca ctgggaatag
     5581 ccacgggaac cgatcaaaag gaacccgacc acgatcattg ctgaatacat acgcttcatg
     5641 tcaataaaat taacgaaaat cgttccaatt tatgataccc ggacaggggg agccctatat
     5701 ttgcatactc aactgattga catgaaaata tgtatgggat tctgtttgct tttgctgatc
     5761 gccgcaggcc ttcgggccca gacccctccc tccatagcgc aaatgaaggc cgatctcgaa
     5821 aaatcgccca atccggtcct atatgccaaa caggttctca aaaaaagatt ccggatcgat
     5881 accatcgttg tcagccgcac ccataatttc gatggcctcg ccgatagcct tgcctattat
     5941 ggaaaggaaa agaaggtgta cggccccttt ggcccccgca acaaccgctt cctcgtgcag
     6001 gtgctcagca aagcccccaa tcagttctat cacgtcggac agatattcat cgatacatcg
     6061 gtattccgat accgcgtcgc cgattccctg ggtaccatga tcctggaaaa actacgcagc
     6121 ggcgccgcca gtttcggaca gatggcggaa gcctattcca tgggagggga ggccgcgacc
     6181 aaaggcgatc tgggctgggt cgcccgcggc gtcctgcaac ccactttcga gcatgaaatg
     6241 ctctttcata aaaaaggaga agtctttaag gtctggagcc gcagagggct gcatatcatc
     6301 aagaagatcg acgacccgaa acaggacacg ggtttcacgc tgatgctgca ggtattctta
     6361 taactggcct cccgctccat cgtcgacagg ctccatcgtc aacaagctcc atcgtcgaca
     6421 ggctccatcg ccccggattt gcctcaccca cgcaaaagat accgcaccgc cagctcatat
     6481 cccttcaatc ccaaacctgc gatcgtcccc ttcgccttag ccgacacatg cgagagacgc
     6541 cggaattcct cccgcgcgtg tacattactg atatgcacct cgaccaccgg agcctttatc
     6601 gccgcaatgg catccccgat agccaccgaa gtatgcgtat atccgcccgg attcaggatg
     6661 atgccgtcgg atgaaaaacc ggaccgctgc aattcatcga tcaactctcc ctccacattg
     6721 ctctggaaat accggaagct gacttccgga aattgccgct gcagctccgt aaaatactgc
     6781 tcgaatgtct ggctgccgta tacctcgggt tcacgcctcc ccagcagatt cagattcgga
     6841 ccgttgatga tggttatttg catgttctta tcatttcgat gaattcatcg aacagatagc
     6901 ggctgtcatg cggacccgga gtagattccg gatgatactg cacggaaaaa gccggccggt
     6961 ccttcagcct tatcccttct atcgacgaat cgttcaggtt gacatgcgtg atctcgacat
     7021 tcttcgcttt cttcaccgcc tccgggtcga ccccgaatcc atgattctg
//
LOCUS       DASYSV010000358         7202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2247332, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000358 DASYSV010000000
VERSION     DASYSV010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247332"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VGQ51_10925"
     CDS             complement(<1..1415)
                     /locus_tag="VGQ51_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEV9037128.1"
                     /translation="MKPIISRLRKLNIEISLVAGKLRIHAPEGVLTGELLEEIKASKD
                     YLIEYILEVRGNRPDTGIPPAEKKRYFRLTSAQRRLYTLYAMDRDSLAYNETQVIRLD
                     GDFQEDKLRRVFRQLVQRHESLRTYFELVENEPVQRIAGAIEPAIGFLEGEESWLDRI
                     IASFIRPFDLHRAPLFRAGLVTTSAGGHFLIVDTHHIITDAFSEGILIRDFRTLWKGE
                     SLDPLPLQYKDYAEWQGHMHRMKGIGQREFWLREFAGEPKPPELPADFPRPAVKGRTT
                     GVLHFELQAAETAALRDIGRESGASLFMILLSIYTILLAKLSGQEDIVVGIPMADRQH
                     AGLEGVMGLFINTLALRNYPRGDKGYLEFLSDLRTRTLACLDHPYYPYEDIIDDLRIA
                     RTTGRNPLFDVWFIYQNYEKAGQDGHGIVLLESRRAERESKFDIILEASDPGSQIFLT
                     IVYSGDLFRQSTMERFAGYFR"
     gene            complement(1448..>7202)
                     /locus_tag="VGQ51_10930"
     CDS             complement(1448..>7202)
                     /locus_tag="VGQ51_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MupA/Atu3671 family FMN-dependent
                     luciferase-like monooxygenase"
                     /protein_id="HEV9037129.1"
                     /translation="NPSNSQDFYRLWEDLGTRRITPHRIIHGWALHRRKDPSFHPAEF
                     SDICATYLFSVLHLVKAARQHDRLSIREIVVLTNDLYNITGYEDAAPAKSISLGLLKV
                     ISQEYPVISTLHIDLSLQGRAVQPTTEALLAEIFRKGTGRTVALRGARRWLQEYVAVP
                     FFARNVVEKPLFRHRGVYLITGGLGSLGFVWSRFLQRKYQATIILVGRSSLEEIDATP
                     EVQSKLRMLRLLQQEEGAVSYYSCDVSDLSGMRQLVCEVEERFGRIDGVVHAAGMVKG
                     RSIAPIATLEEGDFSAQFLPKITGLMVLQEALAGREIGCRLVISSLSPILGGLEFGAY
                     ASANSYMDHLVQAMSADPKTRWTCLNIDGLNLKGGQGEGIRADELETVLEMLPAYMGL
                     SQIVVSVADLNRRLDQWIRREADPVEEPDISGAVEGAAPEGSVTDRLIQLWKKFFGVT
                     HIGPEDDFLELGGDSLKALTMVGRIYREFNVELPVKDLFNHSTIRNLSARIADQGKPA
                     DTKTYHSIPIAAAMESYSLSSVQRRLYFLYEFDKSSTAYNQALTLKLNGRVDGDKIGE
                     VFRQLIRRHESLRTSFGMVGEEPRQQVAEDWLFNVEVLKADGDEDKLIRDFIRPFNLG
                     KAPLIRAGLIEVAAEQYILVVDMHHIITDAVSKGVLMKDFMALYAGEQLPAPRLQYKD
                     FVQWQREDRQQEEMKRKKAFWFREFSEVLPPMELPADHPRPSVKTYDGDAYSFELDAS
                     LTTSLTALAEKEGSTLFMVLLTAYTVLLSRLAMQDDIVVGTYTAGRQHPDLESILGMF
                     VNTLPLRFRVSRHLAFRELLAQVKTRVLQCFDNQSYPYEELIDDLKWERDASRNPLFE
                     ALFSYRNYEHTALELPGLRIGEYPRVKVVVNFDLTLTAIRQRDILSLEFEYATRLFNR
                     DTIGRFAGFFKKIILALVDTPETAIGAIDILSGAQRQQLLFGFNQTQGVYPDTETIVS
                     LFERQASKTPDAVAIVYEGKQLTFRELDETSRRLAVFLRERYGIAPGKTVAIMVQRSE
                     WLLVGLLGILMAGGAYLPIDAGYPESRIAYILEDSGARVLLTDRGSGKPPAGQYENID
                     VEDAAIYAAHPLPLKRTHTPQDLCYLIYTSGSTGKPKGVMITHKNVVNFFSAMNERLG
                     DTVSGCMLAVTSASFDISVLELLWTLCRGMQVVLHPSDISLTTLDRYVVEEKGTVDFS
                     LFFFSSYHFDEQDKYRLLLESVRYADRHGFKAVWTPERHFHEFGGLYPNPSVVSAALA
                     MVTSKIELRSGSIVAPLHDPLRIAEEWAVADNLSNGRIGLSFASGWNPADFVLATTDY
                     KDRQDTLYSQLDEVKKLWKGETVLRRNGLDREVEVRVFPAPVQRELPVWITSGGSEES
                     FIRAGAAGANLLTHLLGQDITELARKISLYRQALRENGHHENKAIVTLMLHAYIGEST
                     AEVEQAVQAPFTEYLKSSIGLSRVFNEESGLKEEDIPEQEKERMIQNAFRRYYKTGSL
                     IGTQTSCREMISRVRAAGVDEIACLIDFGVDPQKVLEGLERLREVKDLVNSASRRHAP
                     VTMLQSTPSFIRLSLESPGSQQLLSSLQYLLLGGEPVPLSLVQRLRKEAPDTRIFNMY
                     GPTETTIWSCAGELMKDADKITIGTPILNTQIYILDNDLQLLPIGVAGEVYIGGEGLS
                     QGYWERPELTAERFIANPFVKGGCIYRTGDIGRWLPDGTIELQGRRDQQVKVRGYRIE
                     LGEIEQVLCAYAHVKEAVVAARDGKDGEKYLAAYVVAEGLQDLSGLREYVSLHLPQYM
                     VPGHFVRLENLPLTPNGKIDRKALPDPEIGPAYADVAPSNETEKKLAALWSEILKLDE
                     KVIGVRSSFFEMGGHSLNALVLIRRIARDFQVEILLQKIFVLQTIERIADVIDNTRWV
                     LGQTREEVTRGEEVILE"
BASE COUNT         1550 a         2143 c         1863 g         1646 t
ORIGIN      
        1 tccctgaagt aaccggcaaa cctttccata gtggattgcc ggaacaggtc ccccgaatag
       61 acgatggtca aaaagatctg tgatcccggg tcggatgctt cgaggatgat atcgaatttc
      121 gattcccttt ctgcccttct ggattcgaga aggacgatgc catggccgtc ctgcccggcc
      181 ttttcataat tctggtagat gaaccatacg tcgaacaggg ggttgcggcc cgttgtgcgg
      241 gctatccgca gatcgtcgat gatatcctca tacggatagt agggatgatc cagacatgca
      301 agcgtcctgg ttctcagatc cgacaggaat tcgagatatc ccttgtcgcc ccggggatag
      361 ttgcgcagcg ccagcgtgtt gatgaacaat cccatgacgc cttcgagacc ggcgtgctga
      421 cgatcggcca tggggatacc gactacgatg tcttcctggc cgctgagctt cgccagtaaa
      481 atggtgtaga tggaaagcag gatcatgaat agagaggcgc cggattcccg gccgatgtcc
      541 cgcaacgcag ccgtttccgc ggcttgcaat tcgaaatgca gcacacccgt ggtccgacct
      601 tttaccgcgg gtctgggaaa gtccgcgggc agctcgggtg gcttcggctc ccccgcgaat
      661 tcccggagcc agaattcgcg ctgcccgatc cctttcattc gatgcatatg tccctgccat
      721 tccgcgtaat ctttgtactg gaggggcagc ggatccaaag attcgccctt ccaaagagtc
      781 ctgaagtcac ggatcaggat cccttccgaa aaggcatcgg taatgatatg atgggtatcg
      841 acgatcagga aatgccctcc ggcagacgtg gtgaccaatc cggccctgaa taagggggca
      901 cggtgcagat cgaagggtcg tataaaggaa gcgattatcc ggtcaagcca ggactcttct
      961 ccttcgagga acccgatagc gggttcgata gccccggcta tacgttgcac cggttcgttc
     1021 tctaccagct caaaataggt acggaggctt tcatgacgct gcaccagctg gcggaacacc
     1081 cggcgcaatt tgtcctcctg gaaatcgccg tccagccgga tcacctgcgt ctcattataa
     1141 gccagcgaat ccctgtccat ggcatacagc gtgtacagcc gtcgttgtgc ggaggtcagc
     1201 cggaagtaac gcttcttttc cgccggcggt ataccagtgt ccggccgatt ccctctcacc
     1261 tccagtatat attcgataag gtagtccttg ctggccttga tctcctccag caattcgccc
     1321 gtcagcaccc cttccggcgc atgtatccga agcttgcccg ccacaagact tatttcgatg
     1381 tttaattttc gcagcctgga aatgatcggt ttcatgacag attgtttgtc ttaagaagaa
     1441 cggtccgtta ctccaggatg acctcctcgc cccgggtgac ctcctccctg gtttgcccca
     1501 gtacccatct cgtattgtcg ataacatccg caattctttc aatggtctgc aatacaaata
     1561 ttttttgcag gagtatttcc acctggaaat ccctggcgat ccgccgtatc agcaccagcg
     1621 cgttcagcga gtgaccaccc atctcgaaaa agctgctgcg aactccgatg accttctcat
     1681 ccaatttcag tatctccgac cagagggccg ccagcttttt ttccgtctca ttggaaggag
     1741 ccacgtccgc ataggcgggt cctatttccg gatcgggcag ggctttccgg tcgatctttc
     1801 cattgggtgt gagtggtaga ttttccagtc gcacgaaatg gccgggcacc atgtattggg
     1861 gaagatgcag gctaacgtat tcccgcagac cggacaagtc ctgcaaccct tccgcgacta
     1921 cataggctgc caggtatttt tcaccgtcct tcccatccct ggcggctact accgcctctt
     1981 tcacgtgcgc ataagcacat agcacctgct ctatctcgcc caactctatc cgatatcccc
     2041 gtaccttgac ctgctggtcc cggcgcccct gcagctcgat cgtgccatcc ggcagccacc
     2101 ggccgatatc tccggtccgg tagatgcacc caccctttac aaaaggattg gcgatgaatc
     2161 gttcggccgt caactccgga cgctcccagt acccttgcga cagtccttca ccgccgatgt
     2221 agacttcgcc tgcgactcct atgggtagca gttgcaggtc attgtcgagt atatatatct
     2281 gcgtattcag gataggcgtg ccgatcgtga tcttgtcggc gtctttcatc agctcgcccg
     2341 cacagctcca gatcgtagtc tccgtaggac cgtacatgtt gaatatccgg gtatccggcg
     2401 cctctttccg cagacgctgt accagcgata gaggtacggg ttcgccgcca agcaggagat
     2461 attgcaggga agagaggagt tgctgtgaac cgggactttc cagcgacagc cggatgaagg
     2521 aaggagtgga ttgcagcatg gtcaccggcg cgtgccggcg gctggcgcta ttgaccaggt
     2581 cctttacctc ccggagtctc tccaaacctt ccaggacctt ttgcgggtct acgccaaaat
     2641 cgatcagaca ggcgatctca tcgactcccg ccgccctgac ccgactgatc atctcccggc
     2701 agctggtctg tgtcccgatc agcgacccgg tcttgtaata gcgtctgaat gcgttctgga
     2761 tcatcctttc cttttcctgt tcgggaatat cttcctcctt caatcccgat tcctcgttga
     2821 aaacacgact gagacctatg ctgcttttga gatattccgt aaacggcgcc tgtacggcct
     2881 gttccacttc cgccgtgctc tcgccgatgt aagcatgcag catcagggta acgatcgcct
     2941 tgttctcatg gtgcccgttc tcccgcagtg cctggcggta caaactgatc ttccgtgcca
     3001 gttccgttat atcctgaccc aacaaatgcg tgagcagatt ggcgcctgcg gcgcccgccc
     3061 tgatgaacga ttcttccgag ccgccggagg tgatccacac cggcagctct cgttgaacgg
     3121 gtgcagggaa aacccggacc tccacctccc tgtccaggcc gttacggcgc aggacggtct
     3181 cgcccttcca caattttttt acctcgtcca gctggctgta gagcgtgtcc tgcctgtctt
     3241 tataatcggt ggtggcaagc acgaaatcgg cagggttcca gcccgacgcg aaagaaagac
     3301 cgatccggcc gttcgaaaga ttgtcagcga cggcccactc ctcggcgatg cgcaaggggt
     3361 cgtgcaaggg tgctacgatg cttccgctgc ggagctctat tttggaagta accattgcga
     3421 gcgctgcact gaccacggat ggattcgggt aaagcccgcc aaactcgtgg aagtgccgct
     3481 ccggcgtcca caccgccttg aaaccatgcc ggtcggcata tctcaccgat tccagcaaca
     3541 accggtattt atcctgttca tcgaaatgat agctggagaa aaagaataag ctgaaatcca
     3601 ccgtcccctt ttcttcgact acgtaccggt ccaaagttgt cagggaaata tccgaaggat
     3661 ggagcaccac ctgcatgcca cggcataggg tccacaacag ctccagaacc gatatgtcga
     3721 aggaggcgct ggtcaccgcc agcatacacc cggacacggt atcccccagc cgttcgttca
     3781 tggcagaaaa gaaattgacg acatttttat gggtgatcat cacccctttc ggtttaccgg
     3841 tggaacccga ggtatagatc aggtagcaca ggtcctgtgg cgtatgtgtc ctctttaggg
     3901 gtagggggtg cgctgcatag atagccgcat cctccacatc gatgttctcg tattgaccgg
     3961 ccggcggttt gccactgccc ctgtccgtca gcaataccct ggcgccgctg tcttccagga
     4021 tatacgctat cctgctctcc ggatagccgg catctatcgg aagataagct ccccccgcca
     4081 tgaggatgcc cagaagcccg accagcagcc attcggaacg ttgcaccatt atagccacgg
     4141 tcttgcccgg cgctatcccg taccgctccc gtaaaaagac agcaagccgg cgggaggtct
     4201 cgtcgagctc tcgaaaggta agctgttttc cctcgtaaac gatagccacc gcatccggcg
     4261 tcttcgacgc ctgacgctcg aacagcgata cgatggtctc tgtatccgga tagaccccct
     4321 gcgtttggtt gaacccgaac aacagctgct gccgctgcgc acccgaaagg atatctatcg
     4381 ccccaatggc cgtttcgggt gtatcgacaa gggccaatat gatctttttg aaaaaaccgg
     4441 caaaacgccc gatcgtgtcc ctgttgaaca gccgggtagc atattcgaac tccagcgaca
     4501 ggatgtcccg ctgtctgatc gccgtcaggg taaggtcgaa atttaccacc accttcaccc
     4561 gtggatattc gccgatacga agaccgggca gctccagggc ggtatgctca tagttccggt
     4621 aggagaacaa ggcttcaaac aaaggattac ggctggcatc ccgctcccat ttcaagtcgt
     4681 cgatcaactc ttcatacgga taagattgat tgtcgaaaca ctgcaatact cgcgttttta
     4741 cctgagcaag gagttcgcgg aaggcaagat gccgggagac ccggaaacgg agcgggagcg
     4801 tattgacgaa catacccaat atactttcga ggtccggatg ctgacgccct gccgtatagg
     4861 tccccacgac gatatcgtcc tgcattgcga gtctggacaa taggaccgta taggcggtca
     4921 acaggaccat gaacagcgtt gagccctctt tttccgcaag cgccgtaagc gatgtcgtca
     4981 gggaagcatc cagttcgaac gaataggcat ccccgtcata ggtcttgacg gaaggacgcg
     5041 gatggtccgc cggcagctcc atgggaggca acacttcgga aaactccctg aaccagaacg
     5101 cttttttacg cttcatttcc tcctgctgtc tgtcctcgcg ctgccattgg acgaaatcct
     5161 tatattgcaa cctcggggca ggcaactgtt ctcccgcata caaagccatg aaatctttca
     5221 tcagcacacc tttggaaaca gcatccgtaa tgatatggtg catgtccacg accaggatat
     5281 attgctctgc cgccacctcg ataaggccgg ccctgatcag tggtgcctta cccaggttga
     5341 agggccggat aaaatcgcgg atcagcttgt cctcgtcacc atcggccttc agtacttcaa
     5401 cgttgaacag ccaatcctcc gcgacctgct gtcgcggctc ctctcccacc ataccgaagg
     5461 aagtgcgaag actttcatga cggcggatca gctgtcggaa tacttcgccg atcttatcgc
     5521 catctacccg gccattcagt ttcagggtca acgcctggtt ataggcggta gacgacttgt
     5581 cgaactcata aaggaaatac aaccttcgct gaacagagct cagggaatag ctttccattg
     5641 ccgccgcgat aggaatagaa tgatatgtct tcgtatctgc cggctttccc tgatccgcaa
     5701 tgcgggcgct caggttacgt atggtggagt gattgaacag gtctttcacc ggcagctcga
     5761 cattgaactc cctgtaaatg cggccgacca tggtcagcgc cttcaacgag tcgccgccaa
     5821 gctccaggaa atcatcctcc ggcccgatat gcgtgacacc aaagaatttt ttccatagtt
     5881 gtatcaatct gtcggttacc gatccttccg gcgcagcccc ttcgacggca ccggatatat
     5941 ccggctcctc taccggatcg gcctcccttc ttatccattg atccagtcgc ctgttgagat
     6001 cggccactga aacaacgatc tgggatagtc ccatatatgc aggcagcatt tccaacaccg
     6061 tttccaactc gtcggctcgg atgccttcgc cctgaccgcc cttcagattg aggccatcga
     6121 tgttcagaca ggtccaccgt gttttcgggt ccgcgctcat cgcctggacc aggtgatcca
     6181 tatagctgtt ggcgctggca taagcgccga attccaatcc ccccaggatt ggtgaaagac
     6241 tggaaatgac gagacggcag ccgatctccc tgcccgccaa cgcctcctgt aataccatga
     6301 gcccggttat cttgggtaag aactgcgcgc taaagtcccc ttcttccagc gttgcgatgg
     6361 gcgctatgga ccgccctttt accatgccgg ccgcatggac gaccccgtct atcctcccga
     6421 atctctcctc cacttcgcac accagctgcc tcatcccgga aagatccgat acgtcgcagg
     6481 aataatatga taccgcccct tcctcctgct gcagcaatcg cagcatgcgc aattttgact
     6541 gcacctccgg cgtcgcatcg atttcctcca gcgacgaacg accgaccagt attatggtag
     6601 cctgatattt tctttgcaga aacctgctcc atacaaaacc aaggctcccc aaaccgccgg
     6661 tgatcaggta gacgccgcgg tggcggaata acggcttttc cacgacgtta cgggcgaaaa
     6721 agggaacggc tacatattcc tgcaaccatc gccgggcgcc tctcagggcg acggtcctgc
     6781 cggttccttt ccggaatatt tccgccagca acgcttcggt agtaggttga acggccctgc
     6841 cctgcagcga caggtcgata tgaagggtgg aaataacagg atactcctgt gagatgacct
     6901 tcagcaatcc gagcgatatg gattttgccg gggcagcatc ttcataaccc gtaatgttat
     6961 acaggtcgtt ggtgagcacg acgatctcgc gtatgctgag cctgtcgtgt tgtcgcgcgg
     7021 ctttcacgag gtgaaggaca ctgaacagat aggtggcgca gatgtcggag aactccgcgg
     7081 gatggaagga cgggtccttt cggcggtgga gcgcccagcc atggatgatc cggtgagggg
     7141 tgatccgtct tgtgcccagg tcttcccata gccgataaaa atcctggctg ttggacgggt
     7201 tg
//
LOCUS       DASYSV010000359         6505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2247371, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000359 DASYSV010000000
VERSION     DASYSV010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247371"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VGQ51_10935"
     CDS             complement(<1..665)
                     /locus_tag="VGQ51_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037130.1"
                     /translation="MKKFLLLIAIPAVVFSCKSKKVSLAVNDEQVNISDFLEAFPKLK
                     LPYEATDTILRRREPDTAVIDDALFERFIPDSILSHLFGKEGHIHLYAVGKIRVPGAE
                     SYVFVKGLAKERRAMYMLVFDKKNHFAAGRPVLYSDNESGVTGKVDMDNKYTLTLLHQ
                     RKASDGKVYYHRDAYVFAEGTGFSLILTETNETKPKPPVIYNPIDTLPHKHRYSGDYA
                     QDKR"
     gene            750..1430
                     /locus_tag="VGQ51_10940"
     CDS             750..1430
                     /locus_tag="VGQ51_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV9037131.1"
                     /translation="MKYSASKENHLKAIFHLQQEQGIVTTNALAAALQTRPASVTDML
                     KKLKEQKLLLYERYKGFRLSNEGRKAAVLVIRKHRLWEYFLVKKLQFGWDEVHVIAEE
                     LEHIGSRELIDRLDAFLGHPETDPHGDPIPDSQGRLPVKRQVSLSDLPLNKVAQVSGI
                     GSQTPEMLEMLNYHHIRLGTRLEIRKRFPFDDSLEVKVRNRPPVTLSARVAKNVLVND
                     DEQGTERR"
     gene            1399..3282
                     /locus_tag="VGQ51_10945"
     CDS             1399..3282
                     /locus_tag="VGQ51_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEV9037132.1"
                     /translation="MMMNKVRNDVSLSEVHGSIDTTRKTSWWRQLFVFFGPAYLVSVG
                     YMDPGNWATDLAGGSRFGYSLLWVLLMSNAMAILLQNLSARLGIVRGRDLAQANREVY
                     PSYVNIPLYILAEIAIAACDLAEVLGMAIGIQLLTGLPLVYGVCITALDTVLLLFLQR
                     LGMRRMETFIIGLIAVVGGSFLVEILLAKPALGDIASGLVPVLKGKQALYIAIGIIGA
                     TVMPHNLYLHSALVQTRKIGSSPADIRRALKINFLDSAVALNLAFFVNAAILILAATV
                     FYNSGRTDVASIKEAYRLLPMLGTQLAPKLFAIALIASGQSSTLTGTLAGQIVMEGYL
                     HIRINPWVRRLLTRLLAIIPAVIVILVSGEEQADNLLVLSQVILSVQLGYAIIPLIHF
                     VSDRSTMGAFTLRPVLRILSWGCAGLLVWLNARLVIQETAPLLAEPGHLALKTGIVIV
                     GLAGLALLGYIILHPFIEARTGRSSPQLHPQPDDLEDWTPPSFRQIAIALDFSEHDEK
                     LLAYAAGQGGNTANYLLIHVVESVSAAMLGRQSDDYETRKDQERLDRYSALLREKGMR
                     SQGVLGYRNRSREIARIVREHHADMLVIGSHGHTGLKDLLYGTTVNSVRHELKIPVLV
                     VSL"
     gene            complement(3353..3964)
                     /locus_tag="VGQ51_10950"
     CDS             complement(3353..3964)
                     /locus_tag="VGQ51_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV9037133.1"
                     /translation="MSDSMASYKAALDGLHRYLSRHVPLTKAEFSQLLPYIEIREFEK
                     KVKVIDKGEIERYLNVIAWGLVRKYLPVRNREITVGLASEGHIVHSELSFHYRTPSGT
                     VIETIEPTVFFSISYDNLQALYDHFPKAERLGRLLISDLYIRKDNRYFDQLLKSTRER
                     FLQYVRTHPQMMQRVPQKYIASYLNIKPETFSRLKHLMRPGRR"
     gene            complement(3975..4565)
                     /locus_tag="VGQ51_10955"
     CDS             complement(3975..4565)
                     /locus_tag="VGQ51_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV9037134.1"
                     /translation="MLELLRQYVSDYAALTDEDFALLAAKIVVRNFDKHVKLIQPGET
                     ERYMNFVTKGLVRMYFYKGRTEVVTVLARERELISASASFLSGEPSNYFVETLEPTTL
                     LSITRDDLEEVYRMSPRVERVGRLMTTQFVLVKEEWEQEYMRLDTRERFIRFVERDPE
                     LIQRVPQKYLASYLNMKPETFSRLKHLLKKRPVNGR"
     gene            4683..5915
                     /locus_tag="VGQ51_10960"
     CDS             4683..5915
                     /locus_tag="VGQ51_10960"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HEV9037135.1"
                     /translation="MAPKIKVSNPVVELDGDEMTRIIWKFIKDKLILPYLDIDIKYYD
                     LGIEHRDQTNDQVTIDAANAIKQYGVGIKCATITPDEARVKEFNLRQMWKSPNGTIRN
                     ILDGTVFREPIVMKNVPRLVTNWTAPIIVGRHAFGDQYRATDTVIKGKGKLTMTFTPA
                     DGGAPQTFEVYDFKGDGVALSMYNTDESVKGFARSCFNMALSKKWPLYLSTKNTILKK
                     YDGRFKDIFEDIYQKEFKQQFQEAGIVYEHRLIDDMVASALKWNGNFVWACKNYDGDV
                     QSDSVAQGFGSLGLMTSVLVTPDGKIMEAEAAHGTVTRHYRDHQAGKPTSTNPIASIF
                     AWTRGLAFRGKLDNNQQLIDFANTLEAVCVEVVEGGKMTKDLAICVHGNKVEHGKHFL
                     YTEEFLDAIDQLLRKKLH"
BASE COUNT         1577 a         1820 c         1611 g         1497 t
ORIGIN      
        1 cgtttgtcct gcgcataatc ccccgaatag cggtgtttat gcggcaaggt atcgatagga
       61 ttatagatga ccggtggctt cggtttggtc tcgttggtct ctgtcaggat cagcgaaaaa
      121 cccgtccctt cggcaaacac ataggcatcc cggtgatagt acaccttccc gtcggaagcc
      181 tttcgctgat gcaggagggt aagcgtgtat ttgttgtcca tatccacctt gcccgttact
      241 ccggattcat tgtcggaata caggacgggc ctgccggccg cgaaatggtt ctttttgtcg
      301 aagacaagca tatacatcgc ccgccgctcc ttcgccagcc ctttgacgaa tacatagctt
      361 tcggcccccg ggaccctgat cttgccgacg gcatacagat ggatatgccc ttctttgccg
      421 aaaagatgcg agaggatgct atcgggaata aaacgctcga acaacgcatc atcgatgact
      481 gctgtatccg gctccctccg tcgcaggatc gtatctgttg cctcataagg cagcttcagt
      541 ttcgggaaag cctccaggaa atcggaaata ttcacctgtt cgtcattgac agcgagggac
      601 acctttttgc tcttacatga aaatacaact gccgggatcg cgatcagcaa caggaatttc
      661 ttcatgggct aaaattaaac aattttagtc cccgaaattc cggattttat ttttagattt
      721 gcctaaaatt tattttagtt tcgccaaaca tgaaatattc cgccagcaaa gagaaccacc
      781 tgaaagccat tttccacctg caacaggaac agggcatcgt cacgaccaac gcactggccg
      841 cggcgttgca gacccgcccg gcttccgtga ccgatatgct aaaaaagctg aaggaacaga
      901 agctgttgtt gtacgaacgt tataaaggtt tccggttgag caatgaagga cggaaggccg
      961 cggtgctggt catccgcaaa caccgtctct gggaatattt ccttgtaaag aaattgcaat
     1021 tcggctggga tgaagtgcat gtgatagccg aagagctgga acacatcggc agccgggaac
     1081 tgatcgaccg gctcgatgcc ttcctgggac atcctgaaac ggacccgcat ggcgatccca
     1141 tccccgacag ccagggaaga ttgccggtca aacggcaggt gagtctttcc gatctgccgc
     1201 tgaacaaggt ggcccaggta tcgggcattg gcagccagac ccctgaaatg ctggaaatgt
     1261 tgaattatca ccatatccgg ctcggtacca ggctggagat ccgtaaaaga ttccccttcg
     1321 atgactcact ggaggtgaag gttcgtaacc ggccaccggt cacgctcagc gcccgcgtag
     1381 ccaaaaacgt attggtaaat gatgatgaac aaggtacgga acgacgttag tctaagtgaa
     1441 gtgcacggga gcatcgatac cacccgcaag acgtcctggt ggcggcaact gttcgttttt
     1501 ttcggtcctg cctatctcgt aagcgtcggt tatatggacc cgggcaactg ggccaccgat
     1561 ctggcgggag gcagccgttt cggctattcg ctgctttggg tcctgctgat gagcaatgcg
     1621 atggcaatcc tgctgcaaaa cctctccgcc cgtctcggca tcgtcagggg ccgcgatctg
     1681 gcacaggcca accgcgaggt ctatccttcc tatgtcaata tcccgctata tatactggca
     1741 gagatcgcca tcgcagcctg tgaccttgcc gaagtactcg gaatggccat cggcatccag
     1801 ttgctgaccg gtctgccact cgtctatggg gtctgcatca ccgcgctcga taccgtcctg
     1861 ctcctttttc tccagcggct gggcatgcga cgcatggaaa ccttcatcat cggtctgatc
     1921 gccgtggtgg gcggctcatt cctcgtggag atattgttgg ccaaacctgc acttggtgac
     1981 atcgcctcgg gtctcgttcc ggtgctgaaa gggaaacagg ccctctatat tgctatagga
     2041 attatcggcg ctacggtgat gccgcataac ctctatctgc actccgcact ggtacagacc
     2101 cggaagatcg gctcctcgcc cgccgacatc cgcagggcgc ttaagatcaa ttttttagat
     2161 agcgccgtgg cactcaatct ggcatttttc gtcaatgcgg ctattctcat cctggctgcc
     2221 accgtttttt acaatagcgg caggaccgac gtagcttcga taaaggaagc ctaccgcctc
     2281 ctgccaatgc tgggaaccca gctggcgccc aagctgttcg caatcgccct gatcgcctcc
     2341 ggccaaagct ctacgctaac aggcaccctc gccggccaga tcgtcatgga aggctatctg
     2401 catatccgga tcaacccttg ggtgaggcga ctcctgacca ggctgctggc catcataccg
     2461 gccgtcatcg tgatcctggt ctccggcgaa gaacaagcgg acaatctcct ggtgcttagc
     2521 caggtgatcc tgagcgttca actgggttac gccatcatcc ccctgatcca tttcgtcagc
     2581 gatagatcga cgatgggcgc cttcacgctc cgtccagtac tccggatact gtcctggggt
     2641 tgtgcgggct tgctggtatg gctcaacgcg agactggtga tacaggagac ggcgccgcta
     2701 ctggccgagc cggggcatct ggcgctcaag acgggcatcg ttatcgtggg tctggcgggt
     2761 ctcgctttgc tcgggtatat aattctgcac ccattcatcg aagccagaac gggtcgaagc
     2821 tccccgcagc tgcatcccca acccgatgac ctggaggact ggacgccgcc ttccttccgg
     2881 cagatagcca tcgccctgga tttcagcgag cacgatgaaa agctgctggc ctacgcggcg
     2941 ggtcagggcg gcaatacggc gaattacctc ctcattcatg tagtggagag cgtttctgct
     3001 gccatgcttg gccggcaaag cgacgactac gagacgcgaa aggatcagga aaggctggac
     3061 aggtatagcg ccctgttgcg cgaaaaaggc atgcgaagcc agggagtatt gggttaccgg
     3121 aaccgcagcc gcgagatcgc ccgtatcgtc cgcgaacacc atgccgatat gctggtcatc
     3181 ggctcgcatg gtcacaccgg gttgaaagat ctcctgtacg gaacgacggt aaattctgtc
     3241 cgccacgagc tgaagatccc ggtattggtc gtgagtttat gaccgtctcc cgcgcgcgac
     3301 cttttgcgcc ccccgggctc ctcgccccaa gcgccccccg ggctctgcac tcctaccgcc
     3361 tccccggccg catcagatgc ttcagccggc tgaacgtctc aggcttgata ttgaggtaag
     3421 acgcaatata tttctgcggc acccgctgca tcatctgcgg atgcgtccgc acatactgca
     3481 gaaaacgctc ccgcgtgctc ttcagcagct gatcgaaata tcggttatcc tttctaatgt
     3541 acagatcgga gatcagcaac cgccccagcc gttcggcctt cggaaaatga tcgtacaacg
     3601 cttgtagatt gtcatacgag atggagaaga atacggtcgg ttcgatggtt tcgatcacgg
     3661 tccctgaagg ggtcctgtaa tggaacgaga gctcggaatg cacgatatgc ccttcggaag
     3721 caagcccgac agtgatctcg cggttccgga ccggcagata tttccgcacc agtccccagg
     3781 cgatgacatt cagataccgt tcgatctccc ctttatcgat gacctttacc tttttttcga
     3841 attcccttat ctcgatgtac ggtaacaatt gactgaactc cgccttagtc agtgggacgt
     3901 ggcgggagag ataacgatgc aatccatcga gagcagcctt gtacgatgcc atggaatcgc
     3961 tcatgggagg ttgtttaacg accgtttaca ggccgctttt ttaacagatg cttcagccgg
     4021 ctgaacgtct ccggcttcat gttcagatag gaggcaagat atttctgcgg cacgcgttgt
     4081 atcagctccg ggtctctctc gacaaaccgt ataaaccttt cacgcgtatc cagccgcata
     4141 tactcctgct cccactcctc tttcaccaac acgaactggg tcgtcatcag acggcccacc
     4201 cgctcgaccc gcggactcat ccggtacact tcttcgagat catcccgggt aatggacaac
     4261 aacgtagtcg gctcaagtgt ctcgacgaaa taattggaag gctccccgga gagaaaagac
     4321 gcagaggcgc tgataagttc gcgctccctg gccaataccg tgacaacttc ggtcctgcct
     4381 ttgtaaaaat acatacgcac gaggcccttt gtcacaaagt tcatgtaccg ctcggtttcg
     4441 ccgggttgga tcagcttgac atgcttgtcg aaattccgga ccacgatctt cgcggccaac
     4501 agagcgaaat cttcgtcagt cagagcagca tagtccgaaa catattgccg aagaagttcg
     4561 agcatgttta aaagcagtta tatgtacaca aacatacgaa aaagagaatt ttgaacgaac
     4621 ctccccaaac tcgccttttt tttataggtt tgctatcttt ttagcgataa aaatcgagca
     4681 ttatggcccc caaaattaaa gttagcaatc ccgtcgtcga actggacgga gacgagatga
     4741 cacgtatcat ctggaaattc atcaaagaca agctgatcct gccttatctg gatatcgata
     4801 tcaaatatta cgatctgggc atcgaacacc gcgatcaaac caatgatcag gtgaccatcg
     4861 acgcagccaa tgcgatcaaa caatatggcg taggtatcaa atgtgcaacc attacccccg
     4921 acgaagcacg cgtaaaagag ttcaaccttc gtcaaatgtg gaaaagcccc aatggcacga
     4981 tccgcaatat cctggatgga actgtcttcc gcgaacccat cgtcatgaaa aatgtacccc
     5041 gcctggtcac gaactggacc gctccgatca ttgtaggacg acatgccttc ggtgaccaat
     5101 accgtgccac ggacaccgtc atcaaaggaa aaggaaagtt gacgatgacc ttcactcccg
     5161 ctgacggtgg cgcgccgcag accttcgagg tctacgattt caaaggcgac ggcgtggctc
     5221 tctcgatgta taacaccgac gaatccgtca aaggtttcgc acgcagctgt ttcaatatgg
     5281 cgctcagcaa gaaatggccg ctttatcttt cgacaaagaa taccatcctc aaaaaatacg
     5341 acggccgctt caaagatatc tttgaggata tctaccaaaa agaattcaaa cagcaattcc
     5401 aggaagcagg catcgtatat gaacatcgcc ttatcgatga catggtcgcc agcgcgctga
     5461 aatggaacgg aaatttcgtt tgggcctgca aaaactacga tggcgacgta caaagcgatt
     5521 ctgttgcaca gggcttcggc tctctcggtc tcatgacctc ggtgctggtc acccccgacg
     5581 ggaagatcat ggaggccgag gcggcgcacg gcacagttac ccgccactat cgcgatcatc
     5641 aggccggaaa gcccacctcc accaacccca ttgcatccat cttcgcctgg acgcgcggcc
     5701 tcgccttccg cggcaagctg gacaataatc aacagctgat cgatttcgcc aataccctcg
     5761 aagccgtttg tgtcgaggtg gtcgaaggcg gaaagatgac caaggacctc gccatttgcg
     5821 tccacggtaa taaggtcgaa cacgggaaac atttcctcta tacggaggaa ttcctcgatg
     5881 cgatcgatca gttactgcga aaaaaactac actaaacaaa aaaggcggcc gttcggccgc
     5941 ctttttaccg cggtatatat tcagcgtgac cttacttctc ttttaatgag taaaggccgg
     6001 tcgttttaat ctgtttttaa tgctagcaat tgcggccaaa cagattctaa tgatttggct
     6061 attataatga tgttttttta acataaatac tattttgtta gataacattt aacatcgcta
     6121 aattcctgcc cgggcaaacc ccgctctgtt atcctccgtt caaactatat tcgtatggac
     6181 acgtctgcac cagcggatta atttctacgc accaaaacga gctgatctat gcgccatcaa
     6241 cctatcgatg ggggctaatc gccccgccat gcctgcagcc taccttgagc atggccaatc
     6301 ttccaaacgg aatcataaac tgccaaccaa aattaaacta ccagtcaatg agaaagtaca
     6361 agttcctgtg gactagtatt ctactactat tccaattgca tttatttgca caaaaccgga
     6421 ctatcaccgg taagatcact gacgtcaacg gtaagccgat accgggcgca agtatcatta
     6481 ttaaaaatac ccacaccggc accag
//
LOCUS       DASYSV010000360        21202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1352603, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000360 DASYSV010000000
VERSION     DASYSV010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 21202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21202
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352603"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..129
                     /gene="mraW"
                     /locus_tag="VGQ51_10965"
     CDS             25..129
                     /gene="mraW"
                     /locus_tag="VGQ51_10965"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEV9037136.1"
                     /translation="MLKAITKKPVTPGDTELKENPRSRSAKLRAAQRV"
     gene            131..463
                     /locus_tag="VGQ51_10970"
     CDS             131..463
                     /locus_tag="VGQ51_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HEV9037137.1"
                     /translation="MTKWKPSLLGYRWIMKNIGFFLFLAVLAVVYIYNGHYADKISRD
                     LTRTDKELRELQYEYKTLKSEVMFRSKQSELAKAVEPFGLKELTHPPYILVDSQVPPE
                     AARPKDNN"
     gene            502..2493
                     /locus_tag="VGQ51_10975"
     CDS             502..2493
                     /locus_tag="VGQ51_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV9037138.1"
                     /translation="MEVKRDILWRVYLCFLGIVALSLVVLGKVVYIQRIQGAHWKGLS
                     DSLHQKFEELDAERGTIYSADSSILSTSLPWFNIYVDFGADGLREKNGRRFTTNLDSL
                     SMGLASLFGDKTAAAYKKQLQKGYRSVDRYYLLQSNVSFEKYRKLRGLPLVREGRNRS
                     GFIAEVLNKRLNPFGLLANRTIGLARTNAQNVGLERTYDTLLKGVTGRRLVRYIAGGA
                     YIPVEGYEIESVNGKDIVTTLDVNIQDIVENALREELQANQAEHGTCIVMEVATGKIR
                     AIANLGLQPDGTYWEDLNYAIRATEPGSTFKLATLLSLLEDKKVSLNDRIDLEGGSWK
                     IGGRTVYDAEPHASKSFSIKEAFEISSNVGMAKLAVAHYAANPGQFIDHLRRLHLDRR
                     TGVDLAGEANPVLITPKSRTWSATALPWMAFGYEILITPLQTLTLYNAVANDGRMMKP
                     YLVSAVEESGNALRTFEPTTLVDPICSESTLHQLQQCLAGVCDDPDGTATGVFKNSFY
                     KVAGKTGTALVANGSHGYAEHIYQSSFVGYFPANAPKYSCIVVIKNKPFAKTFYGAKV
                     AAPVFKEVADRLMSADPDAGIAVTGTPQSLPTPLPPAKPVPAQSIPDVKGMGLEDALY
                     ALESKDLRVSVKGSGKVRSQSPEPGTALQKKETIFIQLD"
     gene            2507..3973
                     /locus_tag="VGQ51_10980"
     CDS             2507..3973
                     /locus_tag="VGQ51_10980"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV9037139.1"
                     /translation="MAQLQEILYKVSIRDIRGVRDRTIERLQTDSRQAGPGSLFIAVK
                     GTHIDGHGFIPQAVASGVTAIVCQDLPGTLQDNVTYVQVEDSAMAAGIIAHNFYGQPS
                     EHLKLVGVTGTNGKTTIATLLFKLFSALDYKCGLLSTVENHIGDRVIPATHTTPDALQ
                     LNALLKQMLDEGCTHVFMETSSHAIHQHRIAGLYFAGGLFSNITHDHLDYHKTFDEYI
                     RVKKSFFDSLPSNAFAISNADDKRGGVMLQNTPAKKYLYSLKTIADFKGRILENNLTG
                     LVMTINDQEVHFRLIGEFNAYNLLAVYGAAICLGLERSEILRCLSTLTGAEGRFDYSV
                     SPKEKIIAIVDYAHTPDALINVLTTIQRLRKGHEKIITVVGCGGDRDKTKRPLMGAAA
                     CEYSDKAIFTSDNPRSEDPDQIIRDMEDGLSTAQKRKYMVITDRKEAVRTALNLAGPD
                     DILLVAGKGHEKYQEIKGVKHPFDDKSVLMEFFELLDK"
     gene            3984..5264
                     /gene="mraY"
                     /locus_tag="VGQ51_10985"
     CDS             3984..5264
                     /gene="mraY"
                     /locus_tag="VGQ51_10985"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV9037140.1"
                     /translation="MLYSLFEWFRQNGIKIPGGALFQFITSRVLLAVVFSLIISMIFG
                     KNLIRALVKKQIGETVRDLGLAGEQQKKGTPTMGGFIIILAILIPTFLLADLHKVYIR
                     LMIFSTIWLGLIGFIDDFLKLRAKRIAKEQGIPYKKGDKDGLAGWFKILGQVVLGVVV
                     GCTLYFNSNTRVWREYVGQTLPSDTTGIKVVTLDKEKKFFVEAKEPITTIPFVKNHEF
                     NYSKLLPTAMRGLTWVLYIVIMIFIITAVSNGANLTDGLDGLATGTSAIMGACLGVFA
                     YASGNIRFAEYLNIMYIPNLGELSIFIGALIGACVGFLWYNAYPAQVFMGDTGSLTLG
                     GIIAALAIIVRKEWLIPIFCGVFLVESLSVIIQVSYFRYTKKKYGDGKRIFLMSPLHH
                     HYQKLGYHESKIVTRFWIVTILCVAFAIATLKIR"
     gene            5402..6742
                     /gene="murD"
                     /locus_tag="VGQ51_10990"
     CDS             5402..6742
                     /gene="murD"
                     /locus_tag="VGQ51_10990"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV9037141.1"
                     /translation="MSKRLVILGGGESGVGAAILAQQRGYDVFVSDGSPLKDDYRNTL
                     KQQDIAFEEGAHTEALILSADEVMKSPGIPEKAAIVKQVRAKDIPVISEIELAYRYKG
                     DSRIIGITGSNGKTTTTAMTYHICRHGGLDCALTGNIGYSLARQVAEDPKPWYVVEIS
                     SFQLDDIMTFRPDVAVLTNITEDHLDRYDYKFGNYIASKFRITMNQTNEDYFIYNEDD
                     PVIQKHIESFQIHSNPLPFTMQQETNNGGFISKGQMNINAGNEKLQMSIYDFALKGIH
                     NQYNTMAAGIASATVGIRKEKIREAIQSFEALEHRMEYVSTVRGVEFINDSKATNVNS
                     TWYALESMEKPTILILGGVDKGNDYSLIRDLVREKVKAIVCMGIDNRKIHEAFQQDVP
                     LMVNTASAAEAVKAAFHFAVKGDVVLLSPACASFDLFKNYEDRGRQFKEAVRDL"
     gene            6749..7993
                     /locus_tag="VGQ51_10995"
     CDS             6749..7993
                     /locus_tag="VGQ51_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV9037142.1"
                     /translation="MSNTTTIERTPFDSLRSRTKGDKVIWTAVFLLALVSLLVVYSST
                     GLLAYKMYKGNTEIYLFKQFAFILVGIGIIYFAHQVNYTIYSRVAAILFVISIPLLLY
                     TLFFGVQLNEGSRWIRLPIINLTFQTSDLAKLALFMYLSRQLSKKQEVIKDFRSGFLP
                     VIIPVGLICLLIAPANLSTALLTGATSLMLLFIGRVRAKHLLLTILIAAIPVALLVGA
                     AALRHGSHGNSFEKPKTEGRSHIFIRVDTWISRVETFIYGGKEESDDNNYQTNQAKIA
                     IANGGFLGLGPGNSRQRNFLPHPYSDFIYAIIIEEYGLAGGLFIVLIYLVFLLRSIRI
                     FRRCPFAFGAFLALGLSFTLVIQALANMAVNVNLFPVTGVTLPLVSMGGSSFLFTCLA
                     IGIILSVARNVEQEHLEGSPDE"
     gene            7986..9086
                     /gene="murG"
                     /locus_tag="VGQ51_11000"
     CDS             7986..9086
                     /gene="murG"
                     /locus_tag="VGQ51_11000"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEV9037143.1"
                     /translation="MNKRIIIAGGGTGGHIFPAVAIAAALQRQDPTIELLFVGAKGKM
                     EMEKIPQAGYRIEGIDIAGFNRTSLIKNAGLPFKLIRSFIQVNRILRRFRPDAVIGVG
                     GYSSFPVLRAAQAKRIPTFIHESNSFAGKSNILLGKKATRIFVASDGMSAFFPADRIL
                     VTGNPVRSNIVQSRITHEEGSRFFGFDPALPIVLSTGGSLGAKGINEAIAAHLDEFAR
                     HETRAQLIWQTGKPFAANAAALCAARKDVWTADFIPQMEYAFAAADVVISRSGAMSIA
                     ELCVAAKPVVFVPFPFAAEDHQTVNAQNLVSKNAGLLVRDAEAKNILVQTVLALCMDK
                     TRQATLKANIGRLAITDAGDRIAADILRTIRQ"
     gene            9083..10456
                     /gene="murC"
                     /locus_tag="VGQ51_11005"
     CDS             9083..10456
                     /gene="murC"
                     /locus_tag="VGQ51_11005"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV9037144.1"
                     /translation="MMEFKDIRTVYFIGIGGIGMSALARYFVFHGKTVSGYDRTETLL
                     TRQLVNESIAIHYEEDLNLIPPHPELVVYTPAVPKDHKELVYYQEHGMTMMKRSEVLG
                     LITNSSFNICVAGTHGKTTISTMIAHILRHSEYGCNAFLGGIAVNYDSNYWSDPRNVC
                     VIEADEYDRSFLRLSPDIAVISAMDADHLDIYGTEEAVMDAFVDFSGRLKPGGTLLTR
                     KGLERESDFRTKNHLTYSSDPDDHADVTAADIRIDQGDYLFSVRMREWSLDHLILTMG
                     GRHNVENATAAIAVAHRLGIGGDKIVAALAAFRGVKRRFEYIIKTPTRVYVDDYAHHP
                     EELRALISGAKELFADMKCTVIFQPHLFTRTRDFADGFAVSLDLADEVFLLPIYPARE
                     LPIAGVSSRLIAEKMTKGKVRQVDKNQLLATLKDKVVVGEGSGLWITAGAGDIDTLIE
                     PIKQLLL"
     gene            10541..11617
                     /locus_tag="VGQ51_11010"
     CDS             10541..11617
                     /locus_tag="VGQ51_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037145.1"
                     /translation="MAIRSHIGKILTTVCWTIAGTAVLVLLVAAIRYRNSNTCKGWRI
                     TIGGATHAGTFIDRKDITDLLLAAGADEHQRRPIQSFDLRRLETALRKDIWIKDAQLF
                     FDNNGILQVKVYERIPVARIFTREGNSFYVDSVGVQLPLQDQFAARLPVFTDYPAAKF
                     RMQGVDSALSAGMLQLSVFLRSDPFWMTRIEQVDITPQKCFELEPAIGDHRIGFGDGR
                     DIEAKFHRLFLFYSEVLSRVGMEKYDRIDVSYAGEVVARQKGLSQNRIDSLRGIDNIR
                     QMIRSAQQLQPDTLRQQHTRPLEDHNTMTEQNLTNFDLVPDAGDSSATVPIRKPAASS
                     DGRPANTPAVPHHRKPRAILPPTP"
     gene            11644..13017
                     /gene="ftsA"
                     /locus_tag="VGQ51_11015"
     CDS             11644..13017
                     /gene="ftsA"
                     /locus_tag="VGQ51_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV9037146.1"
                     /translation="MNLEQPIIVGLDIGTTKIAAIAGRKNEYGKLEILGFGRANSNGV
                     KHGQVLNIDETIKAIRMALENCYASNPNLSITEVYVGIAGHHIKSLQTRGDIVRQNTE
                     EEITRREIDQLVADQYKTYIPAGDQIIDVIPQEFTVDNFQNIPNPIGYGGVKVGANFH
                     IITGDKNAIRNINRAVEKSDLHTKDLVLQPLASAAAVMGQEDLEAGVAIVDIGGGTTD
                     LAVFYEGILKHTAVIPFGGENITNDIKTGLGVLKTQAEQMKVQFGSALANEAKANAYI
                     TIPGLRGMPAKEISVKNLANIIQARMSEIMDFVTYHLKQVGLDNRMLNGGVVMTGGGS
                     QLRHLIQLTEYITGLNARIGFPTEHLAAGHIDELAKPTYSTCIGLILKGYSDYEHNRK
                     EFEKEYRKVEVPDNLRRTETHTHTAVTEEAVQQERVRNRKGMKDFWGKFKDGIIDLFK
                     EEEDRAL"
     gene            13062..15038
                     /gene="ftsZ"
                     /locus_tag="VGQ51_11020"
     CDS             13062..15038
                     /gene="ftsZ"
                     /locus_tag="VGQ51_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV9037147.1"
                     /translation="MIHFDLPKEKSSIIKVIGVGGGGSNAVNHMFSQNIEGVNFIICN
                     TDAQAIAQSRVPNKIQLGPHLTQGLGAGANPSIGRQATEESLEEIKGILEVNTKMAFI
                     TAGMGGGTGTGGAPIISKICKDLGILTVGIITTPFAYEGRKRMLQAEEGIRTMKQYVD
                     TLLVISNDKLRHQFGNLKMKEAFAKADNVLATAAKCITDVINSTGQINVDFADVCTVM
                     RNGGVAILGSATAKGDSRAQKAIEEALNSPLLNDNDISGAKWILININSSEGENEFTM
                     DEVDIIQNHLLSRAGEHTDVILGLGYDNSLGSSLGITLIATGFEHRDPFQIPSETQPA
                     IQKEEKIVMTLKTPIEEIKDTRPVLPSEAPQQPAPSAAQPAQPVAQQIQLNLEPAQPA
                     AQPAAPQLHPHAPHVTPPNPNPRHQPFPVMEQEKPSAPSPETLPTSSLRIEWVLAPEP
                     EIPPVPKAKDPAASAASNRSSASAASGGYLARPSNIYAEDNWNEKQSPPAVADGSPKA
                     IPEHNHSFKEPTLNTPVPDPSKQEDASPDMQLVFRDTVPTADEPGAHQTHSLSVPNSP
                     VEEPAAQDDADDLKRRAAERINKLRNLSFNVNAADPNNEFETVPAYIRRNLELYNTIS
                     NVENFYSNYEVKTDENNNTEISTINTFLDGKRPD"
     gene            15144..16466
                     /locus_tag="VGQ51_11025"
     CDS             15144..16466
                     /locus_tag="VGQ51_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HEV9037148.1"
                     /translation="MPLLLSFIAIACLILLIVWIKLDTFISFTIVCIALGLASGLDIR
                     HIIDAIQKGIGNIMGSLVIILGFGAMLGKLIADSGAAQQITESLVRLSGTRRIQWAMA
                     LSGFLVGLPMFYSAGFMVLMPLVFAVGASTGLPLLYLGIPVLAALSVAHGFLPPHPSA
                     TAIAEQLHADIGKTLLYGIIVATPTIILSGIVFGRTLRRYRPKPNPAVLTIRLMAPGQ
                     LPSLPISLLAALMPLILLTVTAVGGLLPADAPPVIRKTIDFIGNPDIAMLISVLAAIW
                     LLGLRRQRHMRDLMRSLEDAFKGIAPVLLIIAGAGAFMQVMTEGGVNTYIAKTLTATS
                     ISPLIAGWCVAALIRICTGSSTVAALTAVGLLLPLFQHSGTPPELMVLAVGAGSMTFS
                     HINDGGFWLFKEYFNLTIRQTFATWTIMESMISVVGLLMVLLLNQIVH"
     gene            16469..17428
                     /locus_tag="VGQ51_11030"
     CDS             16469..17428
                     /locus_tag="VGQ51_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEV9037149.1"
                     /translation="MSTILITGGTGLIGTALTAMLTENGHRVLLLSRHAPGRSTSTPA
                     SPTSRSPELFHWNPAVGTIDSEAIRQADYIIHLAGANVAERRWTAKQKRLIEESRTQT
                     CTLLVKALTEIPNNVRAVISASAIGWYGPDPAIPNPRPFEETDPADKDFLGETCRKWE
                     ESIGPVATLGKRLVIIRTGIVLSKKSGAFREFLKPVRLGIAAILGSGRQYIPWIHIED
                     ICRLYLHAIEHEDCTGVYNGVAPQPVDNGTLMTNLAKRLKGRYFVPVYIPSFFLKIVI
                     GEMSIEVLKSTTVSAEKVRHAGFQFLYPSIDSALDDLLKDRSS"
     gene            complement(17409..18218)
                     /locus_tag="VGQ51_11035"
     CDS             complement(17409..18218)
                     /locus_tag="VGQ51_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037150.1"
                     /translation="MLQLLRVEWLKIRGYRAFWILMGMTLLSIPGLTYLLFDLMKNSF
                     PKGGPGKAILGSPFAFPDVWQTVSWNSSLLFILPAILLITLTTNEFTYKTHRQNIIDG
                     WSRQQFISVKLVEVLILALKCTAIVFITTLIFGCLANKVPAGVWIWQDSRFLFFYFVQ
                     ALSYFTIAFIMALLIKRAGLSIAVFLIYMLAENIVVGVFRNLYKLNGVDYLPEEVTDR
                     LIPTPYLRKVLQTPADMSKWEHHIPIYLGVAALYLIIYCLVAGRRFTTSDL"
     gene            complement(18230..19123)
                     /locus_tag="VGQ51_11040"
     CDS             complement(18230..19123)
                     /locus_tag="VGQ51_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9037151.1"
                     /translation="MKILSIQAVTKYYGRIRALHEVSFDVPAGSVFGVLGPNGSGKTT
                     LLGIVMDVLKATSGGYSWFGEPPSEKQRRQIGTLLETPNFYHYLSGEDNLRIAAAIKG
                     RGKEDIPRVLEAVKLSERKDSKFSTYSLGMKQRLALASCLLGDPKVLVFDEPTNGLDP
                     VGIAETRELIKVLAREGKTILLASHLLDEVEKVCTHVAILQKGRLLTAGDVNEVLLNE
                     DFIELGAGGVTTAELQRLLQPLPGVKQIKVQDEHVQVYFVSDAMDPSEVNRYCFEKGI
                     ALKYLQLRKKSLESKFMELTK"
     gene            complement(19161..19823)
                     /locus_tag="VGQ51_11045"
     CDS             complement(19161..19823)
                     /locus_tag="VGQ51_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M10"
                     /protein_id="HEV9037152.1"
                     /translation="MGEAELDTSAHELIIRSVLCFYGGAADQTLAIQIADDVSRAWNE
                     PGSKIRIKGEWVTVRFDIEGRYNPDLRPASVWHNTDPRLNFFRIEEYSLHDISSVDGL
                     GCNTGYFKLGNLLNHSTTAAHEYGHTLGLDHPKDLDIRGKGQPGIMYPRGTLCDPAYQ
                     YDPAASAGGPGGTLNPFTRKVLESDIAGLRLSRLPFDRKSLPIVGEFSSWYHEKHAAV
                     EK"
     gene            complement(19878..>21202)
                     /locus_tag="VGQ51_11050"
     CDS             complement(19878..>21202)
                     /locus_tag="VGQ51_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV9037153.1"
                     /translation="KAGEFLRKFHFGLFSYIGHRIPEISDELYRVDDAMMAGFGWEIG
                     AFESWDLLGVAATVKKMKEAGYSVASWVDEMLAAGHKSFYKIEGGRRWYYDIATRKYV
                     SLPGGGAFLVMKNFEQSAVWKNSACRLYHLGDEVLGLEWNTKMGTIGGEVLEGIQRSI
                     GLAEEKYKGLVIAGDGANFSAGANVGLIFMLAIEQDYDELDMAVRQFQQTMMRARYSA
                     VPVVVAPHGLTLGGACELSLHADRVVPAAESYIGLVELGIGVIPGGGGTKEFTLRAAD
                     EMHEGEPETITLKDRFFTVATARVSTSAYEAFDLGILRKGRDIVSINQGRRIAEAKSS
                     VIELYDDGYTMPIPRKDVRVLGRSALGALYAGINGMWRGHYATDHDVVVAKKLAFVMC
                     GGDLSEPTLVTEQYLLDLEREAFLSLCGEKKTLERIQSVLKTGKPIRN"
BASE COUNT         5097 a         6209 c         5425 g         4471 t
ORIGIN      
        1 ttcggacaaa caaagacacc ccccctgtta aaagcgatca caaaaaaacc ggtgaccccc
       61 ggggataccg aattgaaaga aaacccgcga tcgcgtagcg caaagctacg tgccgcacaa
      121 cgtgtataaa atgaccaaat ggaaaccatc tctgctcggc tatcgatgga tcatgaagaa
      181 catcggcttc ttcctgttcc tggccgtgct ggcggtggtc tacatctata acggccatta
      241 tgccgataag atctcgcgcg atctgacgcg caccgataaa gagttgagag aattgcaata
      301 cgaatacaaa acgctgaaaa gcgaagtgat gttccggagt aaacaaagcg agctggccaa
      361 ggcggttgag cccttcgggc ttaaagagct gacgcatcct ccgtacattc tcgtcgacag
      421 ccaggtgccg ccggaggcgg ctcgtccgaa ggacaataat tagatggggg ccgccgtaag
      481 gcggctcaaa atcaaaagga gatggaagtc aaacgcgaca tattatggag ggtctacctc
      541 tgctttcttg gcatcgtggc gctgagcctc gtcgtgctgg gaaaggtcgt gtatatccag
      601 cggatacagg gcgcccactg gaaaggcctc agcgacagcc tccaccagaa gttcgaggag
      661 ctggatgccg aacgcggcac catctacagc gcggacagct cgatcctcag cacttcgctg
      721 ccctggttca atatctatgt cgatttcggc gccgatggcc tccgggaaaa gaacggccgc
      781 cggttcacca ccaacctcga ttcactttcg atgggtctgg cctcgctgtt cggcgacaag
      841 accgcggccg cgtataaaaa gcaattgcag aaaggctatc gttcggtcga ccggtactac
      901 ctcctccaat cgaacgtttc cttcgagaaa tatcgcaagc tccgcggcct gcctctcgtc
      961 cgcgagggtc gcaaccgcag cggctttatt gccgaggtac tgaataaaag attgaatccg
     1021 ttcggcttgc tggccaatcg taccattggc ctggcaagaa cgaatgcgca aaacgtaggg
     1081 ctcgaacgca cctacgatac cctgctcaaa ggcgttaccg gccgtcgtct cgtacgctat
     1141 atcgccggcg gcgcctatat ccccgttgaa ggatacgaga tcgagtcggt gaatggaaag
     1201 gatatcgtta cgacgctgga tgtcaatatc caggacatcg tcgaaaacgc cctgcgggaa
     1261 gagctccagg ccaatcaggc cgaacacggc acctgcattg tgatggaggt ggcaacgggc
     1321 aagatcaggg cgattgctaa tctcggtctc caacccgacg gtacttattg ggaggacctc
     1381 aactacgcca tccgcgccac cgaaccggga tcgaccttta agttggcgac cctcctctcc
     1441 ctgctcgaag acaaaaaagt gtcgctgaac gaccggatcg atctcgaagg cggtagctgg
     1501 aagataggcg ggcgcacagt atacgatgcg gaaccccacg ccagcaaatc gttttcgatc
     1561 aaggaagctt tcgagatcag ctccaatgtg ggcatggcca agctggcggt agcccattat
     1621 gctgcaaacc ccggacaatt catcgaccat ctccgccggc ttcatctcga ccgtcgtacg
     1681 ggcgtggatc tcgccggtga agccaaccct gtgctcatca ccccgaagtc caggacatgg
     1741 agcgcgaccg ctctcccgtg gatggctttc ggttacgaaa tcctgatcac tccgctacag
     1801 acgctgaccc tttataatgc ggtcgccaac gacggccgga tgatgaaacc ttatctcgtc
     1861 agcgcagtgg aagaatcggg taatgctctc agaacctttg aaccgacgac ccttgtggat
     1921 cccatctgca gcgaatccac actccaccag ctacagcaat gcctggccgg cgtatgcgac
     1981 gatcccgatg gtacggctac cggcgtattc aaaaattcat tttataaggt ggcgggtaag
     2041 accggtaccg cgctcgtcgc caacggctca catggatacg cggaacatat ttatcaatcc
     2101 tcctttgtag gctatttccc ggccaatgcc ccgaaatatt cctgtatcgt ggtgatcaag
     2161 aacaaacctt tcgcgaagac tttttacgga gctaaagtgg cggcgcccgt tttcaaggaa
     2221 gtggccgaca ggctgatgag cgccgatccg gacgccggca tagcagtcac cggtaccccc
     2281 caatccctac ccaccccgct cccgccagct aaacctgttc ccgcccaatc gatacccgat
     2341 gttaaaggca tgggtttgga agacgccctg tatgccctcg agagtaagga cctccgcgtt
     2401 tcggtaaagg gcagcggtaa agtgagatcg caaagtccgg agccgggtac ggcgttgcaa
     2461 aaaaaggaaa ccatatttat tcaactcgat tgatgtaacc aggagaatgg cgcaactgca
     2521 ggagatattg tacaaagtga gcatccggga tatccgtggc gttcgtgaca ggaccatcga
     2581 acgcctgcaa acggattccc ggcaggccgg cccgggttct ctcttcatcg cagtgaaggg
     2641 cacccatatc gatggccatg gtttcatacc gcaggcggtc gcctccggcg tcacggccat
     2701 cgtctgccag gatctgcccg gtaccctgca ggacaacgtc acctatgtcc aggtggaaga
     2761 ctcggcaatg gcggcgggta tcatcgccca caatttctat gggcagccct ccgaacacct
     2821 gaagctggta ggcgttacgg gaaccaatgg caagacgacc atcgccactt tattgttcaa
     2881 gctcttctcg gcgctggact ataaatgcgg gctgttgagc acggtggaaa atcatatcgg
     2941 cgaccgcgtc ataccggcca cccataccac ccccgatgcc ctccagctca acgccctgct
     3001 gaaacaaatg ttggacgaag gctgtaccca tgtcttcatg gagaccagct cgcatgccat
     3061 ccaccagcac cgtatcgccg gactttactt cgccgggggg ctattcagca acattacgca
     3121 cgaccacctc gattaccaca agactttcga cgaatacatc cgtgtcaaga aatccttttt
     3181 cgactcgctg ccttccaacg ctttcgccat ctcgaacgcc gacgacaaac ggggcggcgt
     3241 catgctgcag aacacgcccg cgaaaaaata tctctatagc cttaaaacga tagccgattt
     3301 caagggccgc atcctcgaga acaacctcac cggtctggtt atgacgatca acgaccagga
     3361 ggtgcatttc cggctgatag gcgagttcaa tgcctacaat ctgctggcgg tatatggagc
     3421 ggccatctgc ctgggacttg aacgcagcga gatccttcgc tgccttagca cactcaccgg
     3481 cgccgagggc cgattcgatt acagcgtatc gccgaaagaa aagattattg ccatcgtcga
     3541 ctacgcccat acgccggatg ccctgatcaa tgttctcact acgatccagc gtttgcgcaa
     3601 aggtcatgag aagatcatta ccgtcgtcgg gtgcggcggc gaccgcgata agaccaagcg
     3661 gccgctgatg ggcgccgctg cctgcgaata cagcgacaag gcgatcttca cttccgacaa
     3721 tccccggagc gaagacccgg atcagatcat tcgcgatatg gaggacggcc tgagtacggc
     3781 gcaaaaaaga aaatatatgg tgatcaccga tcgcaaagag gccgtccgca ccgcgcttaa
     3841 cctggccggt cctgacgaca tcctgctggt tgcggggaaa ggccatgaga aataccagga
     3901 aataaaaggg gtgaaacatc ccttcgacga taaatcggtg ttgatggagt ttttcgaatt
     3961 gttggataag taaacgaaga gacatgctgt attctttatt tgaatggttc agacaaaacg
     4021 gtataaagat accggggggt gcactcttcc agttcattac ctcccgcgta ttgctggccg
     4081 tcgtcttttc tctcatcatc agcatgatct tcggaaagaa cctcatccgc gcactggtca
     4141 aaaaacagat cggggagacg gtccgcgacc tgggcctggc cggcgaacag caaaaaaagg
     4201 gcactccgac aatggggggc ttcatcatta tactggccat cctcattccc accttcctgc
     4261 ttgccgatct gcacaaggtc tatatccgcc tgatgatctt cagcactatt tggctgggtc
     4321 tcatcggttt catcgacgat tttctgaagc tcagggccaa acgcatcgcc aaagagcaag
     4381 gcattcctta taagaaagga gataaggatg gactggcggg atggttcaag atcctggggc
     4441 aggtagtgct gggtgttgtc gtcggctgta cactctattt caatagcaac acgcgggtat
     4501 ggcgcgaata cgtcggacaa actttgccgt ccgataccac gggtatcaag gtcgttacgc
     4561 tggataagga gaaaaagttc tttgtggaag cgaaggaacc tatcacgacc atccccttcg
     4621 ttaagaacca tgagttcaat tattccaagc tcctgccgac cgccatgcgt ggcctgacct
     4681 gggtactcta tatcgtgatc atgatcttta tcatcacggc ggtatccaac ggggccaatc
     4741 tcaccgacgg attggatggc ctggccaccg gcaccagcgc gatcatgggc gcctgtctcg
     4801 gggtgttcgc gtatgccagc ggcaatatcc gtttcgcgga atatctcaac atcatgtaca
     4861 tccccaacct gggcgagctg tccatattta tcggcgcgct catcggggcc tgcgtaggct
     4921 ttctctggta taacgcctac ccggcgcagg tgttcatggg cgatacggga agcctgacgc
     4981 ttggcggcat cattgccgcc ctcgccatca tcgtccggaa ggaatggctg atacccatct
     5041 tctgcggcgt gttcctggtg gagagcctga gcgtaattat acaagtcagc tatttcaggt
     5101 atacgaaaaa gaaatacggc gatgggaaaa ggatcttcct catgagcccc ctgcatcacc
     5161 attatcagaa gctgggatat catgagagca agatcgtgac gaggttctgg atcgtaacga
     5221 tcctttgcgt agcctttgcg atagcgacat tgaagatcag ataaaacggc gttatgaaaa
     5281 accaccgccg cccggtttct accccgcagg cgtcatgaca aaccgatgta aaagaattgt
     5341 ggtgcagtaa tttctatgct tttgaaaaac cccctaaatc cgactcccaa ctggaatcat
     5401 tatgtcaaaa cgtttggtca tactcggtgg tggagaaagc ggtgtaggtg cggcgatact
     5461 ggcgcagcaa cgaggttatg acgttttcgt cagcgacggc agcccgttga aagatgacta
     5521 ccggaacacg ctgaaacaac aagacatcgc cttcgaagaa ggcgcgcaca ccgaagccct
     5581 tatcctaagc gccgacgaag tcatgaaaag ccccggcatc cctgaaaaag cggccatcgt
     5641 caaacaggtc cgtgccaaag acatcccggt catcagcgag atcgagctcg cttaccggta
     5701 taaaggcgat agccgcatca tcggcatcac cggcagcaat ggcaaaacca cgaccacggc
     5761 aatgacctac cacatctgtc gccatggtgg gctggactgc gcgctgacag gaaacatcgg
     5821 gtattcgctc gcccgccagg tggcggaaga cccgaaacca tggtatgtgg tggagatcag
     5881 tagtttccag ctcgacgata tcatgacctt cagaccggat gtagctgtgc tgaccaatat
     5941 caccgaagat catctcgacc ggtatgatta caagttcggg aattacatcg caagcaagtt
     6001 ccggatcacg atgaaccaga ccaatgagga ctattttatt tataacgaag acgaccctgt
     6061 gatccaaaag catattgaaa gttttcaaat tcactctaac ccattaccat ttaccatgca
     6121 acaggaaacc aacaacggag gattcatcag taaaggacaa atgaacatca acgcgggaaa
     6181 tgagaaactg caaatgagca tctatgattt tgccctcaaa ggcattcaca atcaatacaa
     6241 cactatggca gcaggcatcg catccgccac cgtggggatc aggaaggaga agatccggga
     6301 agccatccag agcttcgagg ccctcgagca tcgcatggaa tatgtcagca ccgtaagggg
     6361 agtggaattc atcaacgatt ccaaggcgac caatgtcaac agtacctggt atgcactgga
     6421 aagcatggaa aaacccacca tcctcatcct cggcggcgtc gacaaaggga atgattactc
     6481 gctcatccgc gatctggtca gggaaaaagt gaaagccatc gtctgcatgg gtattgacaa
     6541 tcggaagatc cacgaagcat tccagcagga cgtgccgttg atggtcaata ctgccagcgc
     6601 cgctgaagcc gtcaaggcgg cctttcactt cgcagtcaaa ggcgatgtcg tcctccttag
     6661 ccccgcctgc gccagcttcg atctttttaa aaattatgaa gacaggggac gacagttcaa
     6721 ggaggctgtc cgcgatctat aataagcgat gagcaatacg acgacgatag aaagaacacc
     6781 attcgatagc ctccggtcga ggacaaaagg ggacaaggtc atctggaccg ccgtattcct
     6841 ccttgcgctg gtgtcattgt tggtcgtgta cagcagcacg ggcctgctcg cttacaagat
     6901 gtataaaggc aataccgaga tctacctgtt caagcaattc gcctttatcc tcgtcgggat
     6961 cgggatcatt tacttcgccc accaggttaa ctataccatt tattcaaggg tggcggcgat
     7021 ccttttcgtc atttccattc ccttgttatt atataccctt ttctttggcg tgcagctcaa
     7081 cgaaggcagc cgctggatcc gtctcccgat catcaatctc accttccaga cctccgatct
     7141 ggccaagctg gctttattca tgtacctcag ccggcaactg agcaaaaaac aggaggtcat
     7201 caaggatttt cgaagtggat tcctccccgt gatcatcccc gttgggctca tctgtctgct
     7261 gatcgcaccc gctaacctca gcacggcact gcttaccggc gccaccagcc tgatgctgct
     7321 gttcatcgga cgcgtccgtg ccaaacacct gttgctgacc atcctcattg cggctattcc
     7381 ggttgcgctg cttgtcggcg ccgctgccct tcggcacgga tcccacggca attcgttcga
     7441 gaaaccgaag accgagggac gttcacatat cttcatccgc gtagatacct ggatcagccg
     7501 cgtggaaacc tttatctatg gcggcaaaga agaaagcgac gacaacaact atcaaaccaa
     7561 ccaggctaag atcgctattg ccaatggcgg tttcctgggt ctcggacccg gcaacagccg
     7621 tcaacgtaat ttccttccgc acccttactc tgatttcatc tatgccatca tcatcgaaga
     7681 atatggtctc gcgggcgggt tgttcatcgt gctgatctat ctcgtcttcc tgttgcgcag
     7741 catccggata ttcagaaggt gtccgttcgc tttcggggct tttcttgccc tggggctcag
     7801 ctttacgctg gtcatccagg cgctggcgaa tatggcggtg aatgtcaatt tatttcccgt
     7861 caccggggtc acccttccgc tggtgagcat ggggggcagc tcattcctgt tcacctgtct
     7921 ggccatcggc attatcctca gcgtcgcacg caatgtggaa caagagcatc tggagggctc
     7981 acccgatgaa taaacgcatt atcatagcag gaggtggtac gggcggtcat atcttcccgg
     8041 cggtggcgat tgccgccgca ctacagcggc aagacccaac catcgaacta ttgttcgtcg
     8101 gcgcaaaggg caagatggaa atggagaaga taccccaggc aggctatcgt atcgaaggga
     8161 tagacatcgc gggcttcaat agaacttccc tgatcaaaaa tgcaggtttg cctttcaagc
     8221 tgatccgcag cttcatccag gtgaaccgta tcctgcgcag gttccggccc gatgccgtca
     8281 tcggggtagg aggatattcc agcttccccg tgctgcgggc cgcacaggcg aaaaggatcc
     8341 ccaccttcat ccacgaaagc aattcattcg caggtaagag caatatctta ttggggaaaa
     8401 aagccacccg cattttcgtc gccagcgacg gtatgtcggc cttctttccc gcggaccgca
     8461 tccttgtcac gggaaatccc gtacgcagca acatcgtgca aagccggatc acccatgagg
     8521 aaggatcgcg tttcttcggt ttcgaccccg cgctgcccat cgtgctttcg acaggaggct
     8581 cgctgggagc caaagggatc aacgaagcca tcgcggctca tctcgacgaa ttcgcgcgcc
     8641 atgagacccg ggcccagctg atctggcaga cgggcaagcc attcgcagcc aatgctgccg
     8701 cgctttgcgc ggcgcgaaaa gatgtctgga cggcagattt catcccgcag atggaatacg
     8761 ctttcgccgc cgccgacgtc gtgatctccc ggtctggtgc tatgtccatc gccgaactat
     8821 gtgtcgccgc aaaacccgtc gtcttcgtac ccttcccttt tgcggcggaa gaccaccaga
     8881 cggtgaacgc gcaaaacctg gtaagcaaga acgccggtct cctcgtcagg gacgccgagg
     8941 ctaaaaatat tttggtgcag acggtcctgg ccctatgcat ggacaagaca agacaggcga
     9001 cattgaaagc taatatcggc cgattggcca tcaccgacgc cggcgaccgt atcgcggcgg
     9061 atatattgag gacgatccga caatgatgga gtttaaggac ataaggacgg tatattttat
     9121 cggcatcggt ggcataggga tgagtgcttt ggcccggtac ttcgttttcc acggaaagac
     9181 ggtatcggga tatgacagga cggaaaccct gctgacccgt cagcttgtaa acgaaagcat
     9241 cgccattcac tatgaagaag acctcaacct catacctccg caccccgaac tggtcgtata
     9301 tacgccggcg gtaccgaagg atcataagga gctggtatac tatcaggagc acgggatgac
     9361 aatgatgaaa aggagcgagg tgctgggttt gatcacgaat agttctttta atatatgtgt
     9421 cgccgggacg cacggtaaga cgacgatcag tacgatgatc gcgcatatcc ttcgtcatag
     9481 tgaatatggt tgtaatgcct ttctgggagg catcgccgtc aactatgaca gcaattactg
     9541 gagcgatccc cggaatgtat gtgtcatcga ggcggatgaa tacgatcgta gctttcttcg
     9601 cttgagcccg gatattgcgg tgatcagcgc gatggatgcc gatcatctcg atatctatgg
     9661 cacggaggag gctgtcatgg atgcctttgt cgatttcagc ggccggctga aacccggcgg
     9721 gacgctgctg acacgaaagg gtctggagcg cgaatccgat tttcggacga aaaatcattt
     9781 gacctattcg tccgatcctg acgatcacgc cgatgtcacg gccgcagata tccggatcga
     9841 ccagggcgac tacctattca gcgtccggat gcgcgaatgg agcctcgatc atctgatcct
     9901 gacgatgggg ggacgccata acgtggagaa tgccacagcc gccatcgccg tcgcccaccg
     9961 gctaggtatc ggcggtgata agatagtcgc cgcgctcgcc gccttcagag gcgtcaaaag
    10021 acggttcgaa tatattatca agacgccgac ccgggtctat gtcgatgact atgcgcacca
    10081 tccggaagag ctgagggccc tgatcagcgg cgctaaagaa ttgttcgcgg atatgaaatg
    10141 taccgtcatt ttccagccgc acctgtttac ccgtacccgc gacttcgccg acggcttcgc
    10201 tgtaagcctg gacctcgcgg atgaagtgtt cctgctgccc atctaccccg cccgcgaatt
    10261 acccatcgca ggcgtgagca gcaggttgat agcggaaaaa atgacaaaag gcaaggtccg
    10321 ccaggttgac aagaaccagc tcctggcgac cctgaaagat aaagttgttg tcggcgaagg
    10381 ctccggatta tggatcaccg ccggcgcggg agatattgac acgttgatcg aacccataaa
    10441 gcagctgcta ttgtgaacgg gactattact aacccgggca cgctttgtac ccgtctgacg
    10501 gcccgaaggg ccgttgggcg ggtctcaaaa aaagaaaaaa atggccatta gatcacatat
    10561 cggaaagatc ctgacgaccg tctgttggac gatcgcaggc accgccgtgc tggtcttgct
    10621 ggtggcagcc attcgttatc gcaacagcaa tacctgtaag ggctggcgta tcaccatcgg
    10681 cggcgcaacc cacgcgggta ccttcatcga ccgcaaagac atcaccgatc tgctgctggc
    10741 tgccggcgcg gacgaacacc agcgccggcc tatccaatcc ttcgatctca ggcggctgga
    10801 gacggcgctg agaaaggata tctggataaa agacgcccaa ttgttcttcg acaacaatgg
    10861 aattctgcag gtgaaggtct atgaacgaat acccgtcgct cgcatcttca cccgcgaagg
    10921 caacagcttt tatgtcgaca gcgtaggtgt acagctgccg ttgcaggacc agttcgcggc
    10981 gcggctgccg gtcttcaccg attatcccgc ggcgaaattc aggatgcagg gcgtggacag
    11041 cgctttgtcc gccggcatgc tgcagctcag cgtcttcctc cggagcgacc ctttctggat
    11101 gacccggatc gaacaggtgg atatcacgcc gcaaaaatgt ttcgagctcg agccggcgat
    11161 cggtgatcac cgcatcggtt ttggtgacgg ccgcgacatc gaagccaaat tccatcgcct
    11221 gttccttttc tatagcgaag tgctcagcag ggtaggtatg gagaaatacg accggataga
    11281 cgtttcctat gccggagagg tagtggccag gcagaaaggc ctgtcccaga acaggatcga
    11341 ttcgctgaga ggtatcgaca atatccggca gatgatccgg tctgcacagc aattacaacc
    11401 ggatacactc cggcaacagc atacacgtcc cctggaagac cataacacga tgacggagca
    11461 aaacctgacc aacttcgacc ttgtacccga tgcaggagac agctccgcca ccgtacccat
    11521 tcgcaaaccc gctgcctcat ccgacggccg accggcgaat acaccggccg taccccatca
    11581 ccgtaaaccc agggcgatat tgcccccaac accgtaaaaa acaacgttta aataaactaa
    11641 actatgaatc tcgaacaacc cattatcgtc ggtctggaca tcggaacgac aaagatcgct
    11701 gccatagccg gtagaaagaa cgaatacgga aagctcgaaa tattgggctt cggccgcgcc
    11761 aactccaacg gcgttaaaca cggccaggta ctcaatatcg acgagacgat caaagccata
    11821 aggatggcgc tggagaactg ttacgcttcc aatcccaacc tgtcgatcac agaggtgtat
    11881 gtgggcatcg ccggtcacca catcaagagc ctgcagaccc gcggcgatat agtacgtcaa
    11941 aacacggagg aggagattac ccgccgggag atcgatcagc tcgtagccga tcagtataag
    12001 acctatatcc cggccggcga ccagatcatc gacgtcatac cgcaggagtt caccgtcgac
    12061 aatttccaga atatccccaa ccctatcggt tatggaggcg taaaggtcgg cgccaatttc
    12121 catatcatca ccggtgataa gaatgccatc cggaatatca accgcgcggt ggagaaaagc
    12181 gatctgcata cgaaggacct ggtcctccag ccgctggctt cagccgctgc cgtaatgggt
    12241 caggaagacc tggaagcagg cgtggcgatc gtagacattg gaggaggtac tacggatctg
    12301 gccgtttttt atgaggggat actgaaacac acggccgtga tcccattcgg cggcgagaac
    12361 atcaccaacg acatcaagac aggcctcgga gtcctgaaaa cacaggccga gcagatgaag
    12421 gtccagttcg gttccgccct ggccaatgag gcaaaggcca acgcctatat cacgattccg
    12481 ggtcttcgcg gcatgccggc aaaagagatc agcgtaaaaa acctggccaa tatcatacag
    12541 gcacggatga gcgagatcat ggatttcgtc acgtaccatc tcaaacaggt gggtctcgac
    12601 aaccgcatgc tcaatggagg agtagtgatg acgggcggag gctcacagtt gcggcatctg
    12661 atccagctga ccgaatacat tacggggctt aatgcccgca tcggctttcc tacggaacat
    12721 cttgccgcgg gtcatattga cgagctggct aaacccactt attccacctg catcggtctc
    12781 atcctgaaag gatacagcga ttatgaacac aatcgcaagg agttcgaaaa agaataccgc
    12841 aaggtcgagg tcccggacaa cctgcggcgg accgaaacgc atacccatac cgccgtcacc
    12901 gaagaggcgg tacaacagga aagggtccgc aaccgaaaag ggatgaagga tttctggggc
    12961 aaattcaagg acggtattat cgacctgttc aaggaagaag aagaccgggc gctgtgaggc
    13021 gccctcgagg ctgataatta ttttatttaa ttattttttt tatgatccat tttgatctac
    13081 cgaaagagaa atcatccatc atcaaggtca ttggtgtggg tggtggtggc agcaatgcag
    13141 tgaaccacat gttcagtcag aacatcgaag gggtgaattt tatcatttgc aataccgacg
    13201 cgcaggcgat cgcgcagagt cgcgtgccta acaagataca gctcggcccc cacctgacgc
    13261 agggtctcgg ggccggcgcg aatcccagca tcggcagaca ggccacggag gaaagccttg
    13321 aggagatcaa agggatcctc gaagtgaaca cgaaaatggc attcattacc gccggtatgg
    13381 gtggcggcac cggtaccggg ggcgcaccca tcatctccaa gatctgtaag gatctcggca
    13441 tcctgacggt ggggatcatc accacgccgt tcgcctatga gggccgcaaa cgcatgctcc
    13501 aggcggagga aggcatccgt acgatgaaac agtacgtcga cacgcttctg gtgatcagca
    13561 atgacaagct gcgtcaccag ttcggcaatc tcaagatgaa ggaagccttc gccaaggccg
    13621 acaatgtact ggcgaccgcg gcgaaatgta tcaccgatgt gatcaatagc accggccaga
    13681 taaacgtgga ctttgcggac gtctgcaccg tcatgcggaa cggaggcgtc gccatcctgg
    13741 gtagtgcgac tgcaaaaggc gacagccggg cacagaaggc tatcgaggag gctctcaact
    13801 cgccattgct caacgacaat gacatcagtg gcgccaaatg gatactcatc aatatcaatt
    13861 caagtgaagg tgaaaatgaa ttcacgatgg acgaggtcga tatcatccag aaccacctgc
    13921 tcagccgcgc gggcgagcac accgatgtca tcctgggact tggctacgat aatagtctcg
    13981 gttcatcgct gggtatcacc ctcatcgcca cagggttcga acatagggac cccttccaga
    14041 tccccagcga aacccaaccc gctattcaaa aagaggaaaa gatcgtgatg acgctgaaga
    14101 cgcctatcga agaaatcaaa gatacacggc ctgttctccc ctctgaagcc cctcaacagc
    14161 cggccccgtc ggcagctcag ccagcgcaac ccgtcgctca gcagatccag ctcaacctcg
    14221 agccggcgca acccgccgct cagcctgccg cgccgcaact tcacccccat gcgccgcacg
    14281 tcactccgcc aaatccgaac ccccggcacc aacccttccc cgtaatggag caggagaaac
    14341 cttctgctcc atcgccggaa acccttccaa caagctcact ccgtatcgaa tgggtgctcg
    14401 cccccgaacc ggaaattccc ccggtaccaa aagcaaaaga tccggcggca tccgccgctt
    14461 caaatcgttc ttcggcgtct gcagcgtccg gaggatacct tgcgaggcca tccaatatct
    14521 atgcagaaga caactggaat gaaaaacaat cccctcccgc ggtggccgac ggttctccca
    14581 aggctatacc ggaacacaac cattccttca aggaaccaac cctgaacacc cctgtccctg
    14641 atccgtctaa acaagaggac gcttcacccg acatgcaact ggtattccgg gacacggttc
    14701 cgacggcgga tgagcctggg gcccatcaaa ctcactcgct ttcggtgccg aactcacccg
    14761 tagaggagcc tgccgcgcag gacgatgcgg atgatctcaa acgccgcgca gccgaacgga
    14821 tcaacaagtt gcgaaacctg tcgttcaacg tcaatgctgc cgatccgaat aatgagttcg
    14881 aaactgtgcc ggcctacatc cgccgtaatt tagaattgta caacacgatc tccaatgtcg
    14941 agaacttcta cagcaactat gaggtgaaga cggatgagaa caacaacacg gagatcagca
    15001 cgatcaatac attcctcgac ggcaaacgcc ctgactagag ggcccgacga tagtttgttt
    15061 ttagttgttt tattcccccc gggtctccgg ggggattttt ttaccccctc gtcgcacgcc
    15121 gcctttttcg ctatcttagc gatatgcccc tcctcctctc cttcatcgct attgcctgcc
    15181 tcatcctcct catcgtctgg atcaaactcg ataccttcat ctcctttacc atcgtctgca
    15241 tcgccctcgg tctcgcctcc ggtctcgaca tccgccacat catcgacgct atacagaagg
    15301 gcatcggcaa tattatgggc tcgctcgtga tcatccttgg attcggagct atgctgggca
    15361 aactcatcgc cgacagcggg gccgcgcaac agatcacgga atctttggtc agactatccg
    15421 gtacccgtcg catccaatgg gccatggctt tatccggctt ccttgtcggc ctccctatgt
    15481 tctattcggc gggattcatg gtcctgatgc cgctggtatt cgccgtcggg gcttctaccg
    15541 gtctgccgct gctctatctc ggtatccccg tgttggctgc cctgtccgtc gcacacggct
    15601 tcctgccacc gcatccgtct gctaccgcaa tcgccgaaca gctccacgcc gatatcggca
    15661 aaacattgct atatggcatc atcgtcgcga cgccgaccat catcctttcg ggtatcgtat
    15721 tcggacgaac gctccgacgc taccggccaa aaccgaaccc tgccgtgctc accatccgtc
    15781 tgatggcgcc cggacaactg ccttccctgc ccatcagcct tctcgcggcg ttgatgcccc
    15841 tgatcctcct aacggtaacc gcggtcggcg gtttgctgcc tgccgatgcc ccgccggtca
    15901 tccgaaaaac catcgacttt atcggcaatc ccgatatcgc catgctgatc tccgtactgg
    15961 cggccatctg gctcctcgga cttcggcggc aaagacacat gcgcgacctt atgagatcgc
    16021 tcgaagacgc attcaaaggc attgcccccg tcctcctgat tatcgcggga gccggagcct
    16081 ttatgcaggt aatgaccgag ggaggtgtca acacctatat cgcaaaaacc ctcacagcca
    16141 cctccatttc cccgctgatc gcgggatggt gcgtcgcggc tctcatccgc atctgtacgg
    16201 gttcttccac cgtggcggcg ctgaccgcgg tgggccttct gctacccctg ttccagcact
    16261 cgggcactcc tcccgagttg atggtcctcg cagtcggggc cgggagcatg accttctcgc
    16321 atatcaatga tggcggcttc tggctgttca aggaatattt caacctgacc atccggcaaa
    16381 ccttcgccac ctggaccatc atggaaagca tgatatcggt ggtgggatta ttgatggtat
    16441 tattgctgaa ccaaattgtt cactgatcat gtccactata ctcattaccg gcgggaccgg
    16501 cctgatcgga acggcgttga ccgccatgct cactgaaaac ggtcatcggg tcctcctgct
    16561 ctcgcgccac gcacccggac gctccacatc gactcccgcc tcgccgacat cccgctcccc
    16621 tgaactcttc cattggaacc ccgccgtcgg caccatcgac tccgaagcca tccgccaggc
    16681 ggactatatt atccatcttg ccggtgccaa cgtcgccgaa cgacgatgga cagccaaaca
    16741 aaaacgcctc atagaagaaa gccgcaccca aacctgtacg ctcctggtaa aagcactcac
    16801 ggaaattccg aacaacgtcc gcgccgtcat cagtgcctcc gctatcggct ggtatggccc
    16861 ggatcccgct atccccaatc cccggccctt cgaagagacc gaccctgccg acaaagattt
    16921 cctgggtgag acctgccgaa aatgggagga gtcgatcgga ccggtggcca ccctcggaaa
    16981 aagactggtc atcatccgca cgggcatcgt actgtcaaaa aaatccgggg cattcaggga
    17041 attcctcaag ccggtgcgtc tcggtatcgc tgccatcctg ggcagtggcc gacaatacat
    17101 cccctggatc catatcgaag acatctgccg tctctatctc catgctatcg aacacgagga
    17161 ttgcaccggc gtatacaatg gcgtggcgcc gcaaccggtc gacaacggca cgctgatgac
    17221 caacctggcg aaaaggctga aaggccgata cttcgtaccg gtctatatcc cgtctttttt
    17281 tctgaaaata gtcataggcg aaatgagtat cgaagtgctg aaaagtacca ccgtcagcgc
    17341 ggaaaaagta cgccatgcag gattccaatt cctgtatcct tccatcgaca gtgcgctgga
    17401 cgatcttctc aaagatcgct cgtcgtaaag cgacgccctg caaccaaaca ataaatgatc
    17461 aggtatagcg cggctacgcc caggtagatc ggtatatgat gctcccattt cgacatatct
    17521 gccggcgttt gcaatacttt ccgcagatag ggggtcggga tcagccggtc ggtgacctcc
    17581 tccggcagat aatccacgcc attgagttta taaagatttc gaaacacgcc caccacgata
    17641 ttctcggcca gcatatagat caggaaaacg gcgatcgaaa gaccggcccg tttgatcagc
    17701 agcgccatga taaaggcgat ggtaaaatag gacagcgcct gaacgaaata aaaaaacagg
    17761 aaccggctgt cctgccagat ccaaactccg gccggcactt tattcgccag acaaccgaag
    17821 atcagcgtcg taatgaacac aatcgccgta catttcaatg ccaggatcag cacctccacc
    17881 aatttcaccg agatgaactg ctgcctgctc caaccatcga tgatgttctg ccggtgtgtc
    17941 ttataggtga attcattcgt cgtcagggta atgaggagaa tggccggaag gatgaacagc
    18001 aggctcgaat tccagctgac ggtctgccat acatcgggga aggcaaaagg actgcctaag
    18061 atggcctttc cgggccctcc tttcgggaaa ctgttcttca tcagatcgaa caggagataa
    18121 gtgagtcccg ggatgctcag gagcgtcatg cccatcaata tccagaacgc acgatagcct
    18181 cgtattttca gccactctac ccgcagtaat tgtaacataa tgtaaggggt tattttgtca
    18241 gctccatgaa tttactttca aggctttttt tccgcagctg cagatatttc aaggcaattc
    18301 ccttttcgaa acagtaacgg ttcacttccg aagggtccat tgcatcactc acgaaataca
    18361 cctggacatg ctcatcctgc acctttatct gtttcacgcc cggcagcggc tgcagtaatc
    18421 gctgcaattc ggcagttgtc acgccgccgg ctcccagctc tatgaaatct tcattcagca
    18481 atacttcgtt gacatcgccg gccgtgagca gccggccttt ttgcaggatg gcgacatgcg
    18541 tacatacctt ttccacttca tccagcagat ggctggcaag caggatggtc ttgccttccc
    18601 gggccagtac cttgatcagc tcccgggtct ccgcgatacc caccgggtcg agaccgttgg
    18661 taggctcgtc gaataccagc accttggggt cgcccagcag acaggaggcg agcgccagcc
    18721 gctgtttcat tcccagcgaa taagtgctga acttcgaatc ctttctttcg ctgagcttca
    18781 cggcttcgag tacccgcggg atgtcttcct ttccccggcc cttgatcgcg gccgcgatac
    18841 ggagattgtc ctcgcccgat agatagtggt agaaattggg tgtttccagc aaagtcccga
    18901 tttgccggcg ctgtttctcg gaaggtggct cgccgaacca ggagtacccg ccgctggtgg
    18961 ccttcaggac gtccatgacg atcccgagca aggttgtctt gccgctgcca ttggggccca
    19021 gcacgccgaa tacggatcct gccggcacgt cgaagctgac ttcatgcagg gccctgatcc
    19081 ggccataata cttggtaacg gcttgtatgg atagaatttt cattttccaa ctacagcgtg
    19141 tttctgacct cccgtatccc tcatttctcc accgccgcgt gcttctcatg ataccaggag
    19201 gagaattctc ccactatcgg cagcgacttt ctgtcgaatg gcaaccgcga caacctcagc
    19261 cccgcaatat cgctctccag cacctttcgc gtaaaaggat tcaaagtccc tcccggcccg
    19321 cccgcagacg ccgccggatc atactgatac gccggatcgc acaacgtccc cctgggatac
    19381 atgatacccg gctgaccctt gcctcggata tcgaggtctt tgggatgatc cagccccagc
    19441 gtatgtccgt attcatgcgc ggccgtcgtg gagtggttca acaggttacc cagcttgaaa
    19501 taacccgtat tgcacccgag gccgtccacc gatgagatat cgtgcaggga atactcctcg
    19561 atgcggaaaa aattcagtcg ggggtcggtg ttgtgccaga ccgacgccgg acgcagatcc
    19621 gggttataac gaccctcgat gtcaaaccgg actgtgaccc attctccctt gatccttatt
    19681 ttgctccctg gctcgttcca ggctctgctg acatcatcgg cgatttgtat ggccagcgtc
    19741 tgatccgcag cgccgccata aaaacaaagt acagaccgta tgatcaattc gtgtgcagag
    19801 gtatccaatt ctgcttcacc cataacacaa tatactaatt ctaaattttc cttcgccacc
    19861 ccacgccctt ctttcgccta gttcctgatc ggcttccccg tcttcagcac gctctgtatc
    19921 ctttccagcg tttttttctc gccgcaaaga ctaaggaacg cctcccgctc cagatcgagc
    19981 aaatactgtt cggtgacgag cgtcggttcg ctgagatcgc cgccgcacat gacgaatgcc
    20041 agcttttttg ccaccaccac atcgtggtcg gtcgcataat gtcccctcca catcccattg
    20101 atgcccgcat acaaagcgcc cagcgcgctt ctgccgagaa cccttacatc cttacgcggt
    20161 atcggcatcg tatagccgtc gtcataaagt tcgatgacgg agctcttggc ctccgcgatc
    20221 ctcctgccct ggttgatgct gacgatgtcg cgccctttcc gcaggatccc caggtcgaaa
    20281 gcctcataag ccgatgtgga gaccctcgcc gtcgcaaccg tgaagaaccg gtccttcaac
    20341 gtgatcgtct ccggctcgcc ttcgtgcatc tcgtctgctg cacgaagcgt gaattctttt
    20401 gtgccgcccc caccggggat gaccccgatg cccaactcca ccaggccgat ataggattcg
    20461 gccgcgggaa ctacacggtc ggcatgcagg ctcagctcac aggcgccacc caacgtcaat
    20521 ccatgcgggg ccacgaccac gggcacggcc gaataacggg cccgcatcat cgtctgttga
    20581 aattgtcgga cggccatatc cagctcgtca tagtcctgct cgatagccag cataaagatg
    20641 agacccacat tcgctcccgc gctgaaattc gctccgtcgc cggcgatcac caaccccttg
    20701 tatttctctt cggccagacc gatcgatcgc tggatccctt ccaacacctc tcctccgatg
    20761 gtccccatct tggtattcca ttccagaccc aatacctcgt ctccgagatg atatagccgg
    20821 caggcgctgt tcttccatac cgccgactgc tcgaaattct tcatcaccaa aaaagcaccg
    20881 ccgcccggaa gggaaacata tttgcgtgtg gcgatatcgt aataccaccg ccggccacct
    20941 tctatcttgt agaaactttt atggcccgcc gccagcattt cgtccaccca ggacgccaca
    21001 gaatatccgg cctccttcat ctttttcacc gttgcggcca cgccaagcag atcccagctt
    21061 tcaaatgcgc cgatctccca gccgaaaccg gccatcatgg cgtcatccac acgatacagc
    21121 tcgtcggata tctccggaat gcgatgccca atataggaaa agagaccgaa gtgaaatttc
    21181 cgcaggaatt ccccggcctt at
//
LOCUS       DASYSV010000361        11535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_969212, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000361 DASYSV010000000
VERSION     DASYSV010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11535
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969212"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /locus_tag="VGQ51_11055"
     CDS             <1..1845
                     /locus_tag="VGQ51_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV9037154.1"
                     /translation="YSHLHRAEVQKKIVAGVEGVAPPKTIWAGVMKYFCIPEAESSYF
                     GWFNIIGAIGVIFYSAAIFSLGFAKGAGPFPFLILAFGVLLGLADLITGFSVRYRVNF
                     HFLLFALAFVMGLKETHNVKTIVLKDATGNGYADRPDLETYLRAWLDARVPAGDTTHG
                     LYDAYFILSNGGASRSGYWTASILGSLEDSTVAWAKGLPGGGGTGGPGAGEGAGAGGP
                     GAGGGGSSGSGRGGQAGEGGSPGNDGGGPAGAVGTGYGRFSDHIFCLSGTSGGGVGVA
                     TFFSMLRDKRRLDTGSYVRSARSFLQQDFFTYTVARMLGPDYLNYITHFSSTRDRGRA
                     LEESLEWSSKELDTTNYAVPFSAPFSGFRALDENRRLAMPLLFVNTTRMQDGNPGVVT
                     NLRLDSNLFNRRLDVLTLLDTGKDISLASGAILGARFPYLSPAGNIDNNYFVDGGYFD
                     NSGAGVVQELIQGIIDIVRKDSLNHGRLFQQLRKVRFRVLHIVNSPVGPAPTQFQPVK
                     PINNDLFSPLITIVGAYDMQTTVNDMRLYHFIKELGALGIVADHHRISLYKEVFEWPK
                     DVKTGMLVDTEGVYPMNWFMSERTRVRMDQRLEGQPVLKDLLKEIRGR"
     gene            complement(1965..2687)
                     /locus_tag="VGQ51_11060"
     CDS             complement(1965..2687)
                     /locus_tag="VGQ51_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV9037155.1"
                     /translation="MKLSNNKILITGGASGIGRGMAERFLKEGNTVIICGRRPQPLDE
                     MAEQHPGLIIRQCDLEIPSEREDLYYWIAKQHPDTNVLVNNAGIQNWMSIDDKDFFQR
                     AHQEIVINIEAPLHLTALFLGLQTLTTVMNVTSGLSFTPLTKAPVYSATKAFFHSFTL
                     SLRHLLRSRNIEVIEIVPPALNTDLGGIGIHSEFPPVSDFIEAIFAQLADGKQQLTFG
                     FSEAMTKAGPDQLAEAFGRMNP"
     gene            2758..3693
                     /locus_tag="VGQ51_11065"
     CDS             2758..3693
                     /locus_tag="VGQ51_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEV9037156.1"
                     /translation="MRRTILVFTIAAMCGIGADAQEGSVAPVVGRWDIVVHTPGREVP
                     SWMEIITSGGRRLVGRYVSGGGSARPIARINWANGKMSFSIPPQWENEEQDLSVEGTL
                     RGDSLSGTLTQPNGKQYSWTGHRAPALKHSAEPAWGKPIVLFDGKDLKGWHAMGNDNQ
                     WVAEGGVLKNQKGGSNLVTDEKFRDFKLHIEFRCPKDGNSGVYLRGRYELQIESLEDG
                     QEPPNDVVGAIYGFLGPVEQAAKPADEWQSYDVTLVGRLVTIVFNGKTVMCRGEIPGI
                     TGGALDSNEGEPGPIYLQGDHGPIEFRNIFITPGR"
     gene            3756..4181
                     /locus_tag="VGQ51_11070"
     CDS             3756..4181
                     /locus_tag="VGQ51_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037157.1"
                     /translation="MKNLFLSLLTLPVAALIYSWTAVRPPDSVEIRSGQWPIHLERTT
                     DRPGVNFSLIFRDQSETIENVLDTLDFADVTQLRYFDQGLSALKKGNNGDCARYKDYS
                     ITRSDKKFEGTYYILRADYGQTIFRQPEADIISKAIKEW"
     gene            4268..4717
                     /locus_tag="VGQ51_11075"
     CDS             4268..4717
                     /locus_tag="VGQ51_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037158.1"
                     /translation="MKKLIILSLFIPAFASAQFVLTNTSTVSLMDPRTGKWPLDLQRV
                     VKDSDTSYLLAFRDQQETEEVNMSTLKFGDLSQLRYFMQALTALKNNSTGTTAKFKDY
                     FIKRMDVRKEGEKGKGTIWYTLQCSDGTTTNFDQPEADKMVAAIKTL"
     gene            complement(4742..5584)
                     /locus_tag="VGQ51_11080"
     CDS             complement(4742..5584)
                     /locus_tag="VGQ51_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV9037159.1"
                     /translation="MNRRQFTKTAISSAAALTFYRTIALAASKYMNIPLGFQSWSVKD
                     RLGKDFSGTLKTMADMGYKLIELCSPAGYKDSGFGFLQGMPAANIKRTIEDAGLHCPS
                     CHFGFAELTDHIDESIAFAKAIGMSQMVCSSFGIPKTGTIPQWQAAVDKLNTAAEKIR
                     AAGMQTGYHNHEGEFATVDGQLIYDVIMARFDSRLVKMQFQTEVINLGYKASTYFTKY
                     PGRFISSHLSDWTADKKEVPIGKGIIDWKEFFKAAKTGGVKNFFVEMDFQNFKDSAVY
                     VKGL"
     gene            complement(5595..7121)
                     /locus_tag="VGQ51_11085"
     CDS             complement(5595..7121)
                     /locus_tag="VGQ51_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9037160.1"
                     /translation="MNDHSNINRRRFVKLTTTAAAGITILPRHVLGGKNYVAPSDILN
                     FAYVGTGTEGIREMFPFLSTPNVRVVAICDPQKDAIGYKDWSPNGIRNQIRTMTKKAD
                     WEPGGDNTIPGGRECGKSIVETYYANNNPEFKYKECKAYADFRQLLDKEKGIDAVKIM
                     TPDHLHGVFCMAAMKRGKHVMVHKPLSNRLLEGKKVIEYARNSKVITHLTPWDANGSM
                     DQVMTWINAGIIGNLKEIHNWTNRPVWPQYDTLPTDNSPIPPGFDWDLWLGPEAQRPY
                     SPNYTNMVFRGWYDFGGGSMADMGHYSLWVVFKALELEKPIMIVPNYTHVTAFNGVTP
                     FQIKNDFSFPTACSVRFAYPAKGSRPAVDLFWYDGGIRPATPDELLAQNKELQPEGMM
                     FVGDKGKILAGFQLENPQILSGTRMESAPTTPRRDEVARYTVAIQTFFDAIKSGQQYT
                     GSFREAEDITEAINLYSVALRSRSTLKYDAASRTITNNSDANKYLSREYRSGWDLDTI
                     "
     gene            complement(7252..8382)
                     /locus_tag="VGQ51_11090"
     CDS             complement(7252..8382)
                     /locus_tag="VGQ51_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEV9037161.1"
                     /translation="MAAPLYLEKQRITSLLPMDECISVMEQMFRSLANGDCLQPLRSL
                     MWLPDHSGLLGMMPGYAAASNTSHLPGDHSNPGHRGNPSNPSNPSNPSNPSDPSNPSN
                     PSNPSNPSNPRNPSNPSIPSILGIKIITIFHANHSHGLPSHQGTVILFDATNGTPLLL
                     LDAAEITAIRTAAASAVATRLLSRQNASRLAILGTGEQAEKHIESISLVRNLTDISIW
                     GRSQPHAEDLARRLRPHYQLNFHVATTPQQAVRDADIICTVTSSGTPVLEGATIPDGA
                     HINAVGSCTPSTRELDTAAVLRSRLFTDRYESLFREAGEFLIPKSEGVLSENDVRGEI
                     GEVLTQTRPGRTNPDEITLFKSLGIAAEDLFAARHIYDKLNA"
     gene            8458..10548
                     /locus_tag="VGQ51_11095"
     CDS             8458..10548
                     /locus_tag="VGQ51_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9037162.1"
                     /translation="MTQPKSPAFAEAVPPADSRFWKHNTFIVLAMSCNLINCLYYFAD
                     GMKKSALIEGSGMVILFGFMVLNLRGLLELPKVLSILFVNLHSFSLCYVEGTRQGAYL
                     YLFPFIMAMIFFLRVRKNNLVVTTFVSGTTLNLLAIVLFFPYQASYEQVTDMVANNHL
                     LLNIIINFMLVIVFFFVVLRLLDAKERKNKSEQKFTDTILNTSLDAVFVVDPAFARVH
                     QYNDKAAELFMLEAFDKRREAYPVLDVLGEKMAGRVGVIGEQGNESGDWQGDMVFYRR
                     NRPSFHGFVSMVSFEYSEKHFVKINILDITSLKLAEFETLQAKERAEKAAATRARFMS
                     NMSHELRTPLNAIIGTTHLLIEDHEMLQESEHFKVLKDSSEHMLQLVNAVLDFSKLDE
                     GKLEFIQETFELERMLQQTADSFATAVQQKGIQLALEVGALPHGRRLVGDEMRLKQVL
                     LNLLSNAIKFTEKGSVTLKTTIGDMSGSGAEIYFAVIDTGIGIPAEKQRMIFDSFTQA
                     DAETTRKYGGSGLGLSICKELVRKMGGELRVESAPDKGSVFYFTIRLPFDQQVIIVTK
                     DTLLGLQKLHGVRILLVEDNAVNMKIASRFLDGWGASVDKAENGKVAWELFQQRAYDL
                     MLVDLEMPLMDGKELVQQVRKLDKRIPAIAFTAAVYDNMYEDLENHGFNGWLHKPFRP
                     DEMHRKILKYLIKK"
     gene            10651..>11535
                     /locus_tag="VGQ51_11100"
     CDS             10651..>11535
                     /locus_tag="VGQ51_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037163.1"
                     /translation="MKKVYVLLLALPGVGCTYFSTQEKKGFDYCYTGGHGLYVANYGN
                     GDPQHVYINGTDPVLSPDGTCIAYTDNGAPDHERRIAVLDLEAGKVTVLDTACHNCYG
                     PVWSPDGEYLAYNAFQGSNWGIKYVDKGNLHPAVLAAPTDSLPGYFGPTWSADSRKIV
                     VQNMSAVYIYDLDGKVLRSVPFSQMDTSVSFSSSSTFLLTDKEDKLVCWGEVDEQSDA
                     EEGPPGAVFVYDLGTGKTSRISPKGYDCWGPVVKGDTVFCNGHSRGKGKDNVYRVDLD
                     GGHFKLAYKNRSAMSFAAR"
BASE COUNT         2507 a         2431 c         3799 g         2798 t
ORIGIN      
        1 tatagtcatt tgcatcgggc ggaagtgcag aaaaagatcg tagcgggcgt cgagggggtt
       61 gcgccgccga aaaccatttg ggccggtgtg atgaaatatt tctgtatacc ggaagcggaa
      121 agcagttatt tcggttggtt caatataatc ggtgcgatag gcgtgatctt ttattccgct
      181 gccattttca gcctggggtt tgccaagggt gcgggacctt ttcctttcct gatcctggca
      241 ttcggagtgt tgctggggct tgccgatctg atcacgggat tttctgtccg gtatcgcgtc
      301 aattttcatt tcctgttgtt cgctctggcg tttgtgatgg gattgaagga gacgcataac
      361 gtgaagacga ttgtattgaa ggatgctacg ggcaatggat atgcggaccg gcccgatctg
      421 gagacctatc tccgggcgtg gctggatgcc agggttccgg cgggggatac gacccatggg
      481 ttgtatgatg cctattttat cctttcgaat ggaggggcat cgcggtcggg atactggacg
      541 gcgtcgatat tggggagttt ggaggattcc acggtggcgt gggcgaaggg gctccccggg
      601 ggcggaggaa cgggcggtcc gggggcgggc gagggggccg gtgcgggcgg tccgggggcg
      661 ggaggtggcg gctcatcggg gagtggcaga ggcggacagg cgggcgaggg cggctcgccc
      721 gggaacgatg gtggtgggcc ggccggtgcg gtcgggacgg ggtatgggag attttcggat
      781 catatttttt gtttgtcggg gacgtcgggc ggaggcgtcg gggttgcgac tttcttttcg
      841 atgttgcgcg acaagcgccg gttggatacg gggtcgtatg tccgaagcgc gaggagcttt
      901 ttgcaacagg acttctttac ttatacggtc gcccggatgc tgggcccgga ctatctcaac
      961 tacatcacgc atttcagcag cacgcgggac aggggcaggg cattggaaga atcattggag
     1021 tggtcttcga aggaattgga taccacgaat tacgccgtac cattttctgc acctttttcc
     1081 gggttcaggg ctctcgatga gaacaggagg ttggcgatgc ccttgctgtt cgtcaatacc
     1141 acgcgaatgc aggacgggaa tcccggtgtg gtcaccaatc tgcggctgga ttccaacttg
     1201 ttcaaccgga ggttggacgt gctgactctg ctggacaccg gcaaggatat cagtcttgca
     1261 tcgggggcca tcctgggagc gcgtttcccc tatctgagcc cggccggaaa tatcgacaac
     1321 aattattttg tcgatggcgg ctatttcgat aactccggag cgggagtagt acaggaattg
     1381 atacagggga taatcgatat cgtccggaag gacagcctga atcatgggcg gctgtttcag
     1441 cagttgcgga aagtgcggtt tcgggtattg catatcgtga atagtcccgt ggggccggcg
     1501 ccaacgcaat ttcagccggt gaagccgatt aataacgatc tgttctctcc attgatcacc
     1561 atcgtaggag cgtatgacat gcaaacgacg gtcaacgata tgcggttgta tcattttatc
     1621 aaagagctgg gcgcgctggg gatcgtggcg gatcatcatc ggatctcgct gtataaggag
     1681 gtattcgagt ggccgaagga tgttaagacg ggtatgctgg tggatacgga aggcgtgtat
     1741 ccgatgaatt ggttcatgtc ggagaggacg cgggtgagga tggatcagcg gttggagggg
     1801 cagccggttt tgaaggattt gttgaaggag atcaggggga ggtaatggcc gggcgcaggg
     1861 aatgtgcgct gggacagagt tgccgtggaa gcccgcgatg aacggctcgg gattgccggc
     1921 gcgcgaagcc gaagaagccc gcgagggatc agaggcgccg cagcttatgg attcatacgc
     1981 ccgaaggcct ccgcgagctg gtcggggccg gccttggtca tggcttcgct gaacccgaag
     2041 gtgagctgtt gtttgccgtc cgccagctgt gcgaagatgg cctcgatgaa atcggagacg
     2101 ggaggaaatt cgctgtggat gcctatgccg ccgagatcgg tgttgagggc gggtggaacg
     2161 atctcaatga cttcgatgtt gcgggatctc aggagatgcc ggagggaaag agtaaaggaa
     2221 tggaaaaagg ctttcgtcgc ggaatagacc ggggccttcg tcagcggagt gaaggagaga
     2281 ccggaggtga cgttcatgac cgtagtcagc gtctgaagac cgaggaacag ggccgtcaga
     2341 tggaggggag cctcgatgtt gatgacgatc tcctgatggg cgcgctgaaa gaaatctttg
     2401 tcgtcaatgg acatccagtt ttggatgccg gcattattga cgaggacgtt ggtgtcggga
     2461 tgttgtttgg cgatccaata atacaggtcc tctctttcgg aagggatctc cagatcgcat
     2521 tgtctgatga taagaccggg atgctgctca gccatttcgt cgaggggctg aggccttctt
     2581 ccgcatatga tgacggtatt gccttctttc aggaaacgtt ctgccatccc acggccgatg
     2641 ccggatgcgc cgccggtgat caggatctta ttattggaga gtttcatctt gtcgactttt
     2701 aaatgccgct gtaaaattag gcgaaaattg ccttatattg cctgaaaagc gatgcatatg
     2761 agaaggacaa tattggtttt tacgatcgca gcgatgtgcg gcattggcgc cgatgcgcag
     2821 gaagggtcgg tggcgccggt ggtcgggcga tgggatatcg tcgtacatac gccgggaagg
     2881 gaagtgccgt cctggatgga gatcatcacc tcgggtggca gaaggctggt gggtcgctat
     2941 gtgtcgggtg gtggaagtgc gaggccgatc gcccggatca attgggcgaa cgggaaaatg
     3001 agcttttcga tcccgccgca gtgggagaat gaagaacagg accttagtgt cgaagggaca
     3061 ctgaggggcg atagtctttc gggcacgctg acgcaaccta acggtaagca atattcgtgg
     3121 acgggtcatc gcgcgccggc attgaaacat agtgcggagc cggcatgggg gaaacctatc
     3181 gtccttttcg acgggaagga cctgaagggg tggcatgcga tggggaatga caaccagtgg
     3241 gtcgcggagg gcggcgtttt gaagaatcaa aaaggaggga gtaacctggt gaccgatgag
     3301 aaattcaggg atttcaaatt gcatatagag ttccgttgtc cgaaggacgg caacagcggc
     3361 gtatatctgc ggggccgtta tgaattgcag atcgagagcc tggaggacgg gcaggagcct
     3421 ccgaacgatg tggttggggc gatctatggt tttctcggtc cggtggaaca ggcggcgaaa
     3481 cctgcggatg agtggcagag ttatgatgta acattggtgg gcagactcgt gacgatcgtt
     3541 ttcaatggga agacggtgat gtgtcgtgga gagataccgg ggataacggg cggagcattg
     3601 gacagcaatg agggcgaacc ggggccgata tacctgcaag gtgaccatgg tcctatagaa
     3661 ttcagaaata tttttattac cccggggagg taaggaatgg attaatatgg gcacactatt
     3721 tgttccgtta gagacgtttt agtcccatat actgtatgaa aaatctgttt ctcagcctat
     3781 taactctgcc ggtcgctgcc ttgatctatt cctggacggc agtaaggccg ccggattcgg
     3841 tggaaatccg ttcgggacaa tggcctatcc atcttgagcg gacgacggac agaccgggcg
     3901 tcaatttttc actaattttt agggatcagt cggagacaat tgaaaatgta ttggatacgt
     3961 tggattttgc cgatgtcacc caacttcggt atttcgatca gggtctgtct gcattgaaga
     4021 aaggcaacaa cggggattgt gcgcgttata aggattattc gatcacgcgg tccgataaga
     4081 agttcgaggg aacttattat atccttcgag ccgattatgg gcagacaatt tttcgtcaac
     4141 cggaagcgga tattataagc aaagctatca aagaatggtg atctgtttaa ggaatgtgaa
     4201 agccgccgcg ggacgtacta aagaatgtat ttaatcgtta tactcaatta aaactactgt
     4261 atccactatg aagaagctaa tcattttatc cttgttcata cctgcatttg ccagtgcaca
     4321 gttcgttctc acgaacacgt ctactgtcag cctgatggac ccgcgtacgg gcaaatggcc
     4381 gctggatctg cagcgggtag tcaaagattc cgacacttct tatttgctgg ctttccgcga
     4441 tcagcaggag actgaagaag tgaatatgtc gacgctcaaa ttcggcgacc tttcacaatt
     4501 gagatacttc atgcaggcgc tgacggcgtt aaagaacaac agtaccggta ccacggccaa
     4561 attcaaagac tatttcatca agcggatgga cgtcaggaaa gagggcgaaa aaggtaaagg
     4621 tacgatctgg tataccctgc aatgcagcga cgggacgacg accaatttcg accagccgga
     4681 ggccgacaag atggttgccg cgatcaagac gttgtagcac agccgggtga tagtgaaggg
     4741 gctaaagccc cttcacgtat acagcactat ctttaaagtt ttgaaagtcc atctcgacaa
     4801 agaaattttt gacgcctcct gttttggcgg ctttgaagaa ttccttccag tcgatgatgc
     4861 ccttgcctat gggcacttcc tttttatcgg ctgtccaatc cgacagatgt gaagaaatga
     4921 aacggccggg gtatttcgtg aaatacgtcg aggccttgta gccgaggttg atcacttctg
     4981 tctggaattg catttttacc agcctggagt cgaatcgggc catgatcaca tcgtagatga
     5041 gctggccatc gaccgtggca aattctccct catgattgtg gtagcctgtc tgcatgcctg
     5101 ccgccctgat cttttcggcg gcggtattca gcttgtcgac cgcggcctgc cactgtggga
     5161 tggtgccggt cttagggatc ccgaaagagc tgcagaccat ctggctcata cctatggctt
     5221 tggcgaaggc gatgctttcg tcgatgtgat cggtaagctc ggcgaaaccg aaatggcagc
     5281 ttggacagtg gagaccggcg tcttcgatgg tgcgtttgat attggctgcc ggcatgccct
     5341 gcaggaatcc gaaaccgctg tctttatagc cggcgggcga acaaagctcg atcagcttgt
     5401 accccatgtc agccattgtt ttgagcgtgc ccgaaaaatc ctttcccagc ctgtccttga
     5461 cactccagga ctggaagccg agggggatgt tcatatattt tgatgcagcg agggcgatag
     5521 tccggtagaa ggtcagtgcg gctgcggagc ttatcgcggt ttttgtgaat tgtcggcggt
     5581 tcatgctatt atttttagat cgtgtcgaga tcccagcccg agcggtattc ccgggaaagg
     5641 tatttatttg cgtcggaatt gttggtgata gtacggctgg cggcgtcgta tttcagcgtg
     5701 gagcggctac gcagggcgac tgaatagagg ttgatggcct ccgtgatatc ttcagcttcg
     5761 cggaaggagc ccgtgtattg ctggccgctt tttatggcat cgaaaaaggt ctggatggcg
     5821 acggtgtacc tggcgacctc gtcccggcgg ggtgtggtcg gagcgctttc catacgggtg
     5881 cctgatagta tttgagggtt ttcgagttgg aagccagcga ggatcttgcc cttgtcaccg
     5941 acgaacatca tcccttcggg ttgcagctct ttgttttgtg ccagtagctc gtccggcgtg
     6001 gccgggcgga tgccgccgtc ataccagaag agatcgactg cggggcgtga gccttttgcg
     6061 ggataggcga agcgaaccga gcaggcggtg gggaaggaga aatcgttttt gatctggaag
     6121 ggggtgacgc cattgaaggc ggtgacatgc gtatagttgg gaacgatcat gataggtttt
     6181 tccaactcga gggctttgaa gacgacccag agactatagt gacccatgtc cgccatggag
     6241 ccgccgccaa agtcgtacca gccgcggaac accatgttcg tgtagttggg gctatagggg
     6301 cgttgtgcct cggggccgag ccataggtcc cagtcgaatc ctggggggat cggcgagtta
     6361 tcggttggca gggtgtcgta ctgcggccat acgggacggt tggtccagtt gtgtatctcc
     6421 ttgagattgc cgatgatacc ggcattgatc catgtcatta cctggtccat ggagccattg
     6481 gcatcccaag gagtgaggtg ggtgatgacc ttggaattgc gagcgtattc gatgactttt
     6541 tttccttcga ggaggcggtt actgagcggc ttgtggacca tcacgtgttt ccctcgtttc
     6601 atcgctgcca tgcagaagac accatggagg tgatcgggcg tcataatctt gacggcgtcg
     6661 atgccttttt ctttgtcgag caattgccgg aaatcggcat atgctttaca ttccttgtat
     6721 ttgaattcgg gattgttgtt ggcgtagtaa gtctctacga tggatttgcc gcattcccgg
     6781 ccgccgggga tagtgttgtc gccaccgggt tcccaatcgg cttttttcgt catggtgcgg
     6841 atctggttcc tgatcccatt gggtgaccaa tccttatagc cgatggcgtc tttttgaggg
     6901 tcgcagatgg ctacgacgcg gacattcggc gtgctcagaa agggaaacat ttcgcggata
     6961 ccctcggtgc cggtgccgac ataggcgaag ttgagaatgt cgctgggggc gacgtagttt
     7021 tttcctccca ggacgtggcg gggaaggatg gtaatgccgg cggcggccgt agtcgtcagt
     7081 ttcacgaacc tacgacggtt gatgtttgaa tggtcgttca tatactagca gatttcgtta
     7141 gagatgcaat aaatgtaacg aaatctgtcc atttggcgag cgatggaaaa tcatttggtg
     7201 gagggcggga ggatatgatc aggcgttgtg tgcgagaagg agtggatatg tttatgcgtt
     7261 gagtttgtca tagatgtggc gggcagcgaa gagatcttcg gcggcgatgc caagggattt
     7321 gaagagggtg atctcgtcgg gatttgtgcg gcccgggcgt gtttgggtga ggacctcgcc
     7381 gatctcgccg cggacatcgt tttcggagag gacgccttcg gatttgggga tgaggaattc
     7441 gcctgcctcg cggaagaggg attcatagcg atcggtgaag aggcgcgagc gcaggacggc
     7501 agcggtgtcg agttctcgtg tcgagggtgt gcaggagcct acggcattga tgtgtgcgcc
     7561 atcgggtatg gtggcccctt ccagaaccgg cgtcccggag gaggtgacgg tgcagatgat
     7621 gtcggcgtct ctgacggcct gctgcggggt ggttgcgacg tggaaattga gctggtagtg
     7681 cggtcgcagg cggcgtgcga ggtcttcggc gtggggttgg ctgcggcccc agatggagat
     7741 atcggtgagg ttgcggacga gggatatcga ttcgatatgt ttttcggctt gttcgcccgt
     7801 gccgaggatg gcgagacggg aagcgttttg tcgcgaaagg aggcgggtgg cgacggcgct
     7861 ggcggcggcg gtgcggatgg cggttatttc ggcggcgtcg agcaggagga ggggggtgcc
     7921 gttagtggcg tcgaagagaa tgacggtacc ctggtgggag gggaggccgt gggagtggtt
     7981 ggcgtggaag atggtgatta ttttgatccc gaggatggaa gggatggagg ggttggaggg
     8041 gttgcggggg ttggatggat tggagggatt ggacgggttg gagggattgg agggatcgga
     8101 ggggttggag ggattggagg gattggaggg gttggagggg ttgccgcgat ggccagggtt
     8161 ggaatggtcg cccgggagat gggatgtgtt ggaggcggcg gcgtagccgg gcatcatacc
     8221 cagcaggccc gaatgatcgg ggagccacat cagtgaacga aggggttgga ggcagtcgcc
     8281 gtttgcgagt gagcggaaca tctgttccat gacggaaata cattcgtcca ttggaaggag
     8341 ggaagtaatg cgctgttttt cgagatataa aggggctgcc attgacgcta aaggtttcta
     8401 attattgtgt tgcaaataac tgatttttat tatattttta aagttaaaac ccaatttttg
     8461 acccaaccga aaagcccggc cttcgcagaa gctgttcctc ccgctgactc acgattctgg
     8521 aaacacaata cgtttattgt gctggcgatg agttgcaatc tcattaactg tctgtattat
     8581 tttgcggacg ggatgaaaaa gagcgccctg atcgagggaa gcggtatggt catcctgttt
     8641 gggttcatgg tcctcaacct gagaggattg ctcgaattgc ccaaggtatt gagcatcctt
     8701 ttcgtcaatc tccattcctt ttcgttgtgt tatgtggaag ggacgcggca gggtgcttat
     8761 ctgtacctgt ttcccttcat catggccatg atctttttcc tacgggtgcg aaagaacaat
     8821 ttagtagtga cgacattcgt cagcgggact actttgaatc tgctggcgat cgtcctgttc
     8881 tttccttacc aggcatcgta cgagcaggtg acggatatgg ttgccaataa ccatttgctt
     8941 ctgaacatca tcatcaactt catgctggtc attgtatttt tctttgtcgt tttgcgattg
     9001 cttgatgcca aggagcggaa gaataaaagc gagcagaaat ttacggatac gatcctaaac
     9061 acctcgctgg atgccgtgtt cgtcgtggat ccggcgtttg cgcgggtcca tcaatacaat
     9121 gataaggcgg cggagctgtt catgctggag gctttcgata agagacgcga ggcgtatccg
     9181 gtgttggacg tattgggaga gaagatggct ggtcgggtgg gcgtaattgg agagcagggt
     9241 aacgagagcg gggactggca gggagatatg gtcttttacc ggaggaaccg gccttcattc
     9301 catggttttg tgagtatggt ctcgttcgaa tattccgaaa aacattttgt aaagatcaat
     9361 atcctcgata tcacctcgct gaaactcgcc gaattcgaga cattgcaggc taaggaaagg
     9421 gcggagaagg cggctgcgac cagggcgcgg ttcatgagca atatgagtca tgaattgcgg
     9481 acgccgctga atgccatcat cggcacgacg catttgctta tcgaggatca tgagatgctg
     9541 caggagagcg agcatttcaa ggtattgaag gattcttcgg agcacatgtt gcagctggtg
     9601 aatgccgtgc tggatttcag taagctggat gagggcaagc tggaatttat ccaggagact
     9661 ttcgagctcg aaaggatgtt gcagcagacg gccgattcat ttgcgactgc tgtgcagcaa
     9721 aagggcatcc agctggcgct ggaagtaggt gccctacccc acggacgcag actggtcgga
     9781 gacgagatgc gtctgaaaca ggtgttgctc aatttgctgt ccaatgcgat aaaattcacg
     9841 gagaaagggt cggtcaccct gaagaccacg atcggggata tgagcggatc gggcgcggag
     9901 atctattttg ccgtgatcga tacagggatc gggataccag cggagaagca gaggatgata
     9961 ttcgacagct tcacccaggc ggatgcggag actacgcgga aatatggcgg atcgggtctt
    10021 gggctcagca tctgcaagga gctggtgcgg aaaatgggcg gggagttgcg ggtcgagtcc
    10081 gcgccggata aaggaagcgt attttatttt acgattcgtc tgcctttcga tcagcaggtg
    10141 atcatcgtga ccaaggacac gctgctcggg ttgcagaagc tgcatggcgt aaggatcctg
    10201 ttagtggagg acaatgcggt gaatatgaag atcgcgtcga ggttcctgga tggctggggt
    10261 gcgagcgtcg acaaggcgga gaacggcaag gtcgcctggg agctattcca gcagcgcgct
    10321 tatgatctga tgctggttga tctggaaatg ccattgatgg acgggaagga gcttgtgcag
    10381 caggtgagga agctggataa gagaataccc gctattgcat tcactgcggc ggtgtatgac
    10441 aatatgtacg aggatctgga gaaccatggg ttcaatgggt ggctgcacaa gccgttcaga
    10501 ccggatgaga tgcatcggaa gatattgaag tatttgataa agaaatagtg atagcgcggc
    10561 gcgtgtgagg cgtgaggcgt gagaggcgtg agacgtgagg cgagtgtgac gtgtgaggtg
    10621 cgagacgcgg agcaaattga aatccaactt atgaaaaagg tatacgtatt attgttagca
    10681 ttgccgggtg tagggtgcac gtatttttcg acacaggaaa aaaagggctt cgattattgt
    10741 tatacgggtg gtcatgggtt gtatgtcgcc aattatggta atggcgatcc gcagcatgtg
    10801 tacatcaatg gcaccgatcc tgtcttatcc cccgacggca cctgtatcgc ctatactgat
    10861 aatggagccc ccgatcatga gcggaggatc gccgtattgg acctcgaggc cgggaaggtg
    10921 accgtcctcg atacagcctg tcataattgc tacggaccgg tgtggtcgcc ggatggggaa
    10981 tatctggcct ataatgcgtt tcagggttcg aattggggta tcaagtatgt cgacaagggc
    11041 aatctgcatc ctgcggtgct ggccgcgccg acggatagtc tcccgggtta tttcgggcct
    11101 acctggagtg ccgacagccg caagatcgtg gtacagaata tgtcagccgt atatatctat
    11161 gatctcgatg ggaaggtgtt gagatcggtg ccgttttcac agatggatac ttctgtgtct
    11221 ttttcgagct cgtcgacctt tttgctgaca gataaggaag acaaattggt gtgttggggt
    11281 gaagtagatg aacagtctga tgcggaggaa gggcctccgg gggcggtctt cgtatatgat
    11341 ctggggaccg ggaagacgag caggatctcg ccgaagggat atgattgctg ggggcctgtg
    11401 gttaaggggg atacggtttt ttgcaatggg cattcgcggg ggaaggggaa ggacaatgtc
    11461 tatcgggtgg atctggacgg ggggcatttc aagctggcat ataagaacag gtcggcgatg
    11521 agctttgcgg cgaga
//
LOCUS       DASYSV010000362         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1733904, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000362 DASYSV010000000
VERSION     DASYSV010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733904"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VGQ51_11105"
     CDS             complement(<1..682)
                     /locus_tag="VGQ51_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV9037164.1"
                     /translation="MKALVYDGPGKKSWTDKPQPKIQQPGDVIVKVLKTTICGTDLHI
                     LKGDVPEVTPGRILGHEGVGEIVETGANVSQFKKGDHVLISCITSCGHCEYCRKGMYS
                     HCKDGGWILGHLIDGVQAEYVRIPHADNSLYKVPKGMDEEALVMLSDILPTGFECGVL
                     NGQVQPGDTVAIVGAGPVGLAALLTAQFYSPAEIVMIDLDDNRLEVARKFGATRTINS
                     GKEDVKKAV"
     gene            complement(702..1748)
                     /locus_tag="VGQ51_11110"
     CDS             complement(702..1748)
                     /locus_tag="VGQ51_11110"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037165.1"
                     /translation="MNLSGVTRTSVRQEKPVVIEPSAAIVLPFNPKMTPRHELEALVQ
                     SLQSAAEKQLLGAHSSGQAMPVIRRLQQLFRGLNFSTHKRSVALFASAETAKTIYLDA
                     KVDTRLIIDQPFRVRDLADCKPDGKEYLLLLLSGKQSKMYVSDASGLRLIKSNTPQHL
                     YAYLNEVPEKTGNFCDRNDRHEIMLNKFLHHMDEGLGAVLKAYPLPVFVAATERVGGH
                     FRQLTRHDRNIAGYLYKHCIDASERDLQELLHPMLDNWGQVTSQLLLLQMEKAAEAGK
                     LVVGIEEVRKAARCSNSRIVIIEKEYADTASFYKQDPIDEVVEKVLENGGEIEKLDKN
                     SLAKYGPIALIRYY"
     gene            1919..2701
                     /locus_tag="VGQ51_11115"
     CDS             1919..2701
                     /locus_tag="VGQ51_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV9037166.1"
                     /translation="MKKILLVMNGLDLNMPAVDFACYIAKLSKSHLTGIFLEGDGGEK
                     ALVPAMPDEKTVEASIQRFREACVCRETLSRVHRDRGLPLSEVIGESRFADVIIIDPE
                     LSAWEPDRHTPGRFVKEILRSSECPVLIAPYSFDGIDELVFAYDGTASSVYAIKQFSQ
                     LFPELRTYKVIVFSVRSDDVGVIDDQFKMKEWLNAHFPEVEMALRKGDPANQLFGYLL
                     EKKKAFVVMGAYGRNLLSRFVKPSEARLVVKTIDLPLFITHR"
     gene            2828..3190
                     /locus_tag="VGQ51_11120"
     CDS             2828..3190
                     /locus_tag="VGQ51_11120"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV9037167.1"
                     /translation="MEDFRKVEDLVDHVREYAHVRMDVARLGLAERSSGVIATLISGA
                     ALAAMLAFSFLFICVAAGLLLGRVLDDLVAGFLIVATFDILLGMIIWWSRKSLIRTPV
                     MNAILEELFEKKEGHEKD"
BASE COUNT          677 a          791 c         1004 g          840 t
ORIGIN      
        1 agacggcctt tttaacgtct tctttgccgg agttgatggt gcgggtcgct ccgaatttgc
       61 gggctacttc gagccggttg tcatcgaggt cgatcatcac gatctccgcg ggggaataga
      121 attgggcggt gaggagggcg gccaatccca cgggaccggc gcctacgatc gcgacggtat
      181 ctccgggctg aacctggcca ttgagtacgc cgcattcgaa tcctgtgggt aggatatcgc
      241 tgagcatgac cagcgcctct tcgtccatgc ctttcggcac tttatacagg ctgttgtctg
      301 cgtgggggat ccggacatat tcggcctgca cgccgtcgat cagatggccg aggatccagc
      361 cgccgtcctt acagtgggag tacatccctt tccggcaata ttcacagtgt ccgcaggagg
      421 tgatgcagga gatgagcaca tggtcgcctt ttttgaactg gctgacattt gcgccggtct
      481 cgacgatctc gccgacgcct tcatgaccca ggatacggcc gggggtgact tcagggacgt
      541 cgcctttgag aatatggagg tccgtgccgc aaattgttgt tttcagcact ttgacgatta
      601 catcgccggg ttgctggatc ttcggctggg gtttatcggt ccaggacttt ttgccggggc
      661 catcgtatac caatgctttc atgtagattt attttgaaag gttaataata tcggatcagg
      721 gcgatggggc cgtattttgc aagggaattt ttgtcgagct tttcgatctc tccgccattc
      781 tcgagcactt tttccacgac ctcgtcgata gggtcctgtt tataaaagga tgctgtgtcg
      841 gcgtattctt tttcaataat gactatcctg gaattgctgc aacgggcggc cttgcgcact
      901 tcttctatcc cgacgacgag ttttcctgcc tctgctgcct tttccatttg caggagcaac
      961 aactggctgg tcacctggcc ccagttgtcg agcatggggt ggagcaattc ctgcaagtcg
     1021 cgttcactgg catctatgca atgtttatac agatagccgg cgatattgcg gtcgtgccgg
     1081 gtaagttggc ggaaatgacc accgaccctt tcagtggctg cgacgaacac gggaagggga
     1141 taggctttga ggactgcccc gagaccctcg tccatgtggt gcaggaattt gttgagcatg
     1201 atttcgtggc ggtcgttcct gtcacagaaa ttgccggtct tttccgggac ctcattcagg
     1261 taggcataga ggtgctgagg ggtattgctt ttgatcagcc gtaagccgga tgcgtcgcta
     1321 acgtacattt ttgactgctt gccgctgagg agcaatagga ggtattcttt cccatcgggt
     1381 ttgcagtcgg caaggtcgcg gacgcggaag ggctgatcga tgatcaatcg ggtatcgact
     1441 ttggcatcga ggtaaatggt ctttgccgtc tcggcagatg cgaacagcgc cacgctgcgt
     1501 ttatgggtag agaaattgag gccgcggaat agctgctgca gccgccggat caccggcata
     1561 gcctgaccgg aggaatgggc tcccagcaat tgcttttcgg cagcggactg cagggattgc
     1621 accagcgcct cgagttcatg ccggggcgtc atttttgggt tgaaaggcaa tacgatcgct
     1681 gcggaaggtt cgatgacgac cggcttctcc tgtcgaacgc tggtccgtgt gacacctgaa
     1741 aggttcatgg tcatttattt gaaccaaaga tatttccggg gacatgttcg ggacaatgag
     1801 taccgtcagg ggcggggatg atcgtggtca caattgccgc tcgtgatgac gatcaatggc
     1861 agggctgatg tctctcatgg gatatgagtg gttttctgcg gtcctttaca aaaaaagtat
     1921 gaaaaagata ttgcttgtga tgaatggatt ggatctgaat atgccggcgg tcgatttcgc
     1981 ctgttatata gcgaaattga gcaagtcaca tttgacaggt atattcctgg aaggggatgg
     2041 tggggaaaag gccctcgttc cggccatgcc ggacgagaag acggtggaag ccagtataca
     2101 gcgcttccgg gaggcctgtg tctgtcgcga aacgctatcg cgggtccatc gggaccgggg
     2161 actgccactg tccgaggtga tcggcgaaag ccgcttcgcc gatgtgatca tcatagatcc
     2221 ggagctctcc gcctgggaac ccgaccgtca tacgccggga cgtttcgtaa aagagatact
     2281 gaggtcctcg gaatgtccgg tacttatcgc tccgtacagt tttgacggta tcgatgaact
     2341 ggtgttcgcc tatgacggga cggcttcatc cgtgtatgcg atcaagcagt tcagccagct
     2401 atttcccgag ctgagaacct ataaagtcat cgttttcagc gtccgcagcg acgatgtggg
     2461 agtcatcgac gaccagttca aaatgaaaga atggctgaac gcccattttc cggaagtgga
     2521 gatggcgctg cggaaggggg accctgccaa tcagttgttc ggttatctgc tggaaaaaaa
     2581 gaaggccttt gtcgtcatgg gagcctatgg gcgcaacctg ctgtcgagat tcgtgaaacc
     2641 cagcgaggcg cgcctggtcg tcaagacaat cgatctgccg ctgttcatta cacaccggtg
     2701 actgttagga cgcccgacgg gtcgggtggt cttgtctggt tcagggacat atatcatctg
     2761 cgttcctgac ggaggtcatg agtccaggtt tgcgccgggt gtagatttag gttcaatatt
     2821 taactatatg gaagacttta gaaaagtgga agacctggta gatcatgttc gtgaatatgc
     2881 gcatgtccgg atggacgtgg cgcgtctggg tttggcagaa cggtcgtcgg gcgtcattgc
     2941 gacgctaatt tcgggtgcag ccctggcggc catgctggct ttcagcttct tattcatctg
     3001 tgttgccgcg gggcttctcc tgggacgggt gctcgatgac ctcgtagccg gatttctcat
     3061 cgtggcgacc ttcgatatcc ttttggggat gatcatctgg tggagccgca agagtcttat
     3121 ccggacgccc gtcatgaatg ccatcctgga agagttgttt gagaaaaaag aaggtcatga
     3181 gaaagattaa ccgattgtcg cagttgaggc aagagcaggt gcggcttgtg catcggaggt
     3241 atgagctcga gcacgatatc cggaaagatt ggggcgacct gcggcatcat ttcgaggccg
     3301 cgtcgtttgc cc
//
LOCUS       DASYSV010000363         3884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1602961, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000363 DASYSV010000000
VERSION     DASYSV010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602961"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VGQ51_11125"
     CDS             <1..505
                     /locus_tag="VGQ51_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9037168.1"
                     /translation="LSLEKLEEGVVQNNPKSTNLEKIAQDAILDMEGAWKNGQSIELN
                     ISGLPRPVNVDQHLLRNILSNLLSNAIKYSPEYSPIRLTLEFNEKTIHLIVTDTGIGI
                     PSEEQQHLFERFFRAANTTSISGSGLGLSIVKKYLDLMGGAIAVDSRPGKGSVFTVTL
                     PTDPLPA"
     gene            668..1120
                     /locus_tag="VGQ51_11130"
     CDS             668..1120
                     /locus_tag="VGQ51_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV9037169.1"
                     /translation="MSTKDLNRRESLPSVFNDFFRPWGSVFDTNGGSLFDTLNPVTIP
                     AVNISENKERFEVSLAAPGMKKADFNIDVERNTLTISAETKAEKEEKDERYTRKEYSY
                     SSFSRSFTLPEGVNKDKIDASYDNGVLTLSLPKTEDAKKTASKHISVK"
     gene            1291..2133
                     /locus_tag="VGQ51_11135"
     CDS             1291..2133
                     /locus_tag="VGQ51_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEV9037170.1"
                     /translation="MEAQKVLQSDILDILFDRRNKEYGAYQLRKGYNKRLLLSLSAIA
                     LLTASLFGVNYLSGLHRVKTQKTVYSVVDVQLESIKEENKIAPPPSPPPPPATVQKVE
                     ITKFTPPKIVKDEEVKLDERPPEQEKLQETKIGAINQEGVKDEGITGPPTGETGNKGV
                     VEAPAASKAEDWDRTFTKVEIESDYPGGVAAWIRYLNRNFRYPDDALNNEIHGTVVVQ
                     FVVDKDGLVSDVQVVSGPATGGLREEAMRVIRHSGKWNPAIQNGRKVKSYKQQPIIFE
                     LRNS"
     gene            complement(2181..3257)
                     /locus_tag="VGQ51_11140"
     CDS             complement(2181..3257)
                     /locus_tag="VGQ51_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV9037171.1"
                     /translation="MQKILFIQDQAQKEEDIRQLLGLSNYRVVVAADGREGIEMALGE
                     RPDLILCDTQLSSVDGYGVIHALQHYPETRGIPVVLLMSMAEKDSFREAMKAGADDFL
                     ARPFEGIDLLRAVEACLERHRNRVSGEHGAFMKSGGRLQGEHAHVGTDSREDADWRPD
                     EQDIRSFKKRTLLYLEGQRASQVWYVVSGKVKTYLIHSDGKELITSICGPGDCVACAA
                     AVHGGSYTDNAQVLEDSTLTLIPRPEFLRLLAGNPGFIRQMIKWLSRSSRTQETGLLN
                     LAYSSLRKKVANGILSLWDNFRKEDDGKAYISISRENLAAIIGSAPESLTRTLSDFRR
                     ERLIEITDGKIYLLDEGGLRNMIN"
     gene            3392..3871
                     /locus_tag="VGQ51_11145"
     CDS             3392..3871
                     /locus_tag="VGQ51_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV9037172.1"
                     /translation="MLGTLTTPEIEKLLRKEVIGRLGCADGKMVYVVPISYVYDGEFV
                     YCHTHEGLKVQIMRQHPTVCFEVDRLQNMGNWQSVVSHGQFEELTDPALRNAALQKLH
                     ERILPVISSETTHLSPDWPFAPAEFSKIEGVTFRIRLQEKSGRFERSHTFFQSFFSI"
BASE COUNT          994 a         1145 c          924 g          821 t
ORIGIN      
        1 tctctccctg gagaaattgg aagaaggggt ggtccaaaat aatcccaagt caaccaatct
       61 ggaaaaaatc gcgcaggacg ctatcctcga tatggaagga gcctggaaaa acgggcaatc
      121 catcgagttg aatatttccg gcttaccccg gccggtaaat gtcgaccagc accttcttcg
      181 caatatcctc agcaatctct tgagcaatgc catcaaatat tcgcccgaat atagccccat
      241 ccgtctaacc cttgaattta acgaaaagac catccatttg atcgttaccg acaccggcat
      301 cggaattccc tccgaagaac agcaacatct tttcgaaagg ttcttccggg cggcgaatac
      361 caccagcatc tcgggctccg gtcttgggct gagcatcgta aaaaaatacc tcgacctgat
      421 gggcggcgcc atcgccgtcg atagtcggcc gggcaaaggc tcggtcttca ccgtcacact
      481 gcccacggat ccgctgcccg cttaaaagcg aagtcaccac atccacccac caccgtccct
      541 cagcgagccc tcgctcatag tgaccaggtt catctccgcc actgacgcag attgccttcc
      601 ggctgcctcc gccgaccgaa atttgtgatt gaaattcatt ctcaaacatt caatccaagg
      661 aggcattatg tcaacgaaag acctgaacag gagggaatcg ctcccctctg tattcaatga
      721 tttcttcaga ccatggggct cggttttcga cactaacggc ggatcgctat tcgacacgct
      781 gaacccggtg accattccgg ccgtcaacat ctccgaaaat aaggagcggt tcgaggtcag
      841 tctggccgca cccggaatga aaaaagccga tttcaacatc gacgtcgagc gcaataccct
      901 gaccatcagc gcagaaacca aggccgaaaa agaagagaag gacgaacggt atacccgcaa
      961 ggaatacagc tattcttctt tctccagatc cttcacctta cccgaagggg ttaacaagga
     1021 taagatcgac gccagctatg acaacggggt attgacgctg tcgctgccga agaccgagga
     1081 cgcgaaaaag accgcttcca aacatatttc ggtgaaataa ggcggacgag ttgaagtatg
     1141 gccctgccgt tttcgcgcgg cggggttttt tgtgcatatg actcttgtca tcttccgggc
     1201 taatgacagt cataataccg ccctccgcgg tgctgtagct tagtccgaag aaacataacc
     1261 ctaacccatc accgctaaat cattgactat atggaagccc aaaaagttct gcaaagcgac
     1321 atcctggata tcctgttcga ccgcaggaac aaggaatacg gcgcctacca gctgcgtaaa
     1381 ggctacaata aacggctgct gttgagcctt tccgcgattg ctctcctcac agcttcacta
     1441 ttcggtgtca actacctgtc cggcctgcac agggtcaaga cccaaaaaac ggtttactcg
     1501 gtggtcgatg tccagctcga gtccattaaa gaggaaaaca aaatagcacc tcctccttct
     1561 ccgcccccac caccggcaac cgtccaaaag gtcgagatca cgaaattcac tccgccaaag
     1621 atcgtcaaag acgaagaggt aaaattggac gaacgtccgc ccgaacagga aaagctgcaa
     1681 gaaacgaaga tcggagcgat caaccaggaa ggcgtcaaag atgaaggaat aacagggccg
     1741 ccgaccgggg aaaccggtaa caaaggcgtc gtcgaggcac ccgcggcctc caaagccgag
     1801 gattgggacc gcactttcac caaagtagag atcgaatcgg attacccggg aggcgtcgcg
     1861 gcctggattc gttatctaaa caggaatttc cgctatccgg atgatgcgct gaacaatgag
     1921 atccacggga ccgtcgtcgt gcagttcgtc gtcgataaag acggactcgt cagtgacgtg
     1981 caggtggtca gcggccccgc cacgggcggt ctgcgcgaag aagccatgcg ggtgatcagg
     2041 cacagtggca aatggaaccc ggcgattcag aacggcagaa aggtgaagtc ctacaaacaa
     2101 cagcccatca tctttgaact acgcaactcg taataccgaa gaacaattgc cctgacacaa
     2161 aagaccggac acacatccgg tcaattgatc atatttctca gaccgccctc atcgagcagg
     2221 tatatcttgc cgtccgtgat ctcgatgagc cgctcccttc tgaaatcgct gagcgtccgc
     2281 gtcaacgatt ccggcgccga accgatgata gcggccagat tttcgcgcga tatcgagata
     2341 taagctttcc cgtcatcctc cttgcggaaa ttgtcccata gactaaggat cccattggcc
     2401 acctttttcc gcaacgaact ataggcaagg ttcagcaaac ccgtctcctg ggtcctgctg
     2461 cttcggctca gccatttgat catctgccgg ataaatcccg gattaccggc cagcagccgc
     2521 aggaattccg gacgcgggat gagcgttagt gtcgaatcct ccaacacctg ggcgttatcg
     2581 gtatatgatc cgccatgaac ggcggccgca cacgccacgc aatcgccagg accgcaaatg
     2641 ctcgtaatca attccttccc atccgaatgg atcagatagg tctttacctt cccgctgacg
     2701 acataccata cctgcgaggc gcgctgtcct tccaggtaga gcaaagtcct ctttttaaag
     2761 gaccgaatat cctgctcatc cggtcgccag tccgcgtctt ccctactatc ggtccccacg
     2821 tgagcatgct ccccttgcag ccggccaccg gacttcatga aagcgccatg ttcgcccgag
     2881 acccggttcc ggtgtctttc cagacaggcc tccacggccc tcagcaagtc gatcccttcg
     2941 aaaggcctcg ccagaaaatc atccgccccg gccttcatcg cctctctgaa gctgtccttt
     3001 tctgccatcg acatcaacag gactacggga atgccgcgcg tttcagggta atgctgaagc
     3061 gcatggataa ctccatagcc atcgacggat gatagctgcg tatcacacag gatgagatcc
     3121 ggcctctcgc caagagccat ttcgatgcct tccctgccgt cggcggccac aacgacccgg
     3181 taattggaaa gaccaagcaa ttgcctgata tcttcctcct tctgggcctg atcctggata
     3241 aaaagtattt tttgcatatc caactccatt ccccggcaaa ataccacaca cattgaccct
     3301 cattctatga agtttatcaa ctccgatctt gatccctgtc attgtccgtg aaaacaccac
     3361 gcgctaattt cggatcacaa aatcattcat tatgctggga acattgacca cgcccgaaat
     3421 tgaaaagctg ctgcgcaagg aagtcatcgg ccggcttggc tgcgccgatg ggaaaatggt
     3481 gtatgtcgtc ccgatcagct atgtctacga cggagagttc gtctactgcc atacgcacga
     3541 agggctaaaa gtgcagatca tgagacagca tcccacggta tgttttgaag ttgaccggct
     3601 gcagaatatg ggtaactggc agagcgtcgt atcgcacggt cagttcgagg agctgaccga
     3661 tcctgctctc cgaaatgccg ccctccaaaa gctccatgag cgcatattgc ccgtcattag
     3721 tagcgagacc acgcatcttt cgcccgactg gcctttcgcg cccgcggaat tcagtaagat
     3781 cgagggtgtc accttccgca tccggctcca ggaaaaatcc ggccggttcg agcggtcgca
     3841 tacctttttc caaagtttct tcagcatctg atatcgatat ctat
//
LOCUS       DASYSV010000364         5585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_583804, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000364 DASYSV010000000
VERSION     DASYSV010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583804"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..652)
                     /locus_tag="VGQ51_11150"
     CDS             complement(203..652)
                     /locus_tag="VGQ51_11150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9037173.1"
                     /translation="MTTWTLKEFDQLTPHELYAVLRLRSEVFVVEQNCVFLDMDDKDQ
                     YCHHLLGWRGERLVGYSRIVPAGISYAESSIGRIVTSPAARRRGVGRELMVQSIDTLY
                     SLYGKTDIRIGAQYYLLRFYESFGFVQKGEIYLEDGIEHIEMLLTAT"
     gene            complement(661..1926)
                     /locus_tag="VGQ51_11155"
     CDS             complement(661..1926)
                     /locus_tag="VGQ51_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV9037174.1"
                     /translation="MSSSQAPLAERLRPQTLDQLVGQQHLTGKGSVLRTAIGHGKPPS
                     MILWGPPGTGKTTIAGIIAGTLKVPFYTLSAISAGVKEVREVIAEAKDKSRAILFIDE
                     IHRFNKGQQDALLGAVEKGIVTLIGATTENPSFEVNSALLSRCQVYVLKPLSEEDLGR
                     LLENALRDDEWLRSLDIRLKETKALITLSGGDARKLLNLLEVVADAAGKGATVTDDLV
                     MEIAQQRIALYDKKGEQHYDIISAFIKCIRGSDPNAAVYWLARMIEGGEDVKFIARRM
                     LILASEDIGNANPNALLLANACFEAVNKIGYPESNLILSQCAIYLASSPKSNSATVAI
                     GAAQQAVRSKGDLSVPLHIRNAPTQLMKKMDYGKGYEYSHNYERNFSAQEYLPNELAG
                     TKFYEPGQNAREEELRKFLKSLWGDKYGY"
     gene            2002..3399
                     /locus_tag="VGQ51_11160"
     CDS             2002..3399
                     /locus_tag="VGQ51_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV9037175.1"
                     /translation="MMAGNPVSIRRLTTLVTCLLLANIYCLASGRDPVLSGVQNYALN
                     RPGVVMIRTEYTANVYVNRMTMDDRSFNALLDSIQKLDHGGGVSPEQKLDIMLREMSN
                     RPSRFFRSTFEYIKQPEQITSIGTGFFITADGYVATNCHLIDREDAFIRRQFILAAFR
                     QITEASIAALENSWATHFTEQQRTLLYNTYASVYSRLFTIVLYDLKKNIYVVYRSDDA
                     YRQSDTVKKVADVIVKGQPMPGKDIAILKIEDGGNLPVLRLAGPAMPQVGEQLYVYGY
                     PGPVTNNDFVSAASAIEPTLTTGIVSAIKQSVGDWPVIQMDANINHGSSGGPVCNEKG
                     EVVGLTTFGSIENTGGLAAGLNFAVPVTILDEYVDSAGIDPEPGTATLHFTSALSSYG
                     QRRYSAALTEFLAVQRLNPHYPGIYAYIMDCQEMVKDGKGRPIGPVERVLFIGAGLLL
                     LLGLFSWLRARRAGK"
     gene            complement(3409..3903)
                     /locus_tag="VGQ51_11165"
     CDS             complement(3409..3903)
                     /locus_tag="VGQ51_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037176.1"
                     /translation="MRNTLIIARILTWFNMIFWTGLLGLMLLSALALQLYPALAGVFL
                     LCSIPLNNYAALQLHKAIRYPNVKLSHHTPTGIRFVGLVALFFGIYTVINAYVFLTDP
                     KTPLQIIKQGMPEMKKLPDAQLIGVLHLLGVGSLVVGLAVIVNVVLNTRLLRWYYRIR
                     SDKS"
     gene            complement(3900..>5585)
                     /gene="aspS"
                     /locus_tag="VGQ51_11170"
     CDS             complement(3900..>5585)
                     /gene="aspS"
                     /locus_tag="VGQ51_11170"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV9037177.1"
                     /translation="FGEQENLNKELDRQPLGREFVIQVTGEVAERASRNPQLPTGDIE
                     IKVSSFRILNKAATPPFTIQDETDGGDDLRMKYRFLDLRRPTVKKNLELRYAVNRATR
                     EYLHANGFMDLETPFLIKSTPEGARDFVVPSRMNPGQFYALPQSPQTFKQLLMVSGYD
                     RYYQIVRCFRDEDLRADRQPEFTQIDCEMSFVEQEDILSMFEGMVRHIFKEVKDIDYA
                     GKIERMTWEEAMWQYGNDKPDIRFGMKLLNLKTAFKKGGKDELIAGAGFGVFDNAETV
                     LAIAVPGCAEYTRKQTDELTEWVKRPQIGMQGLVYIKCNMDGSFKSSVDKFYPEPKLK
                     AIAEAANAQPGDLILILAGREEKTRKAISELRLEMGRRLGLRKPDEFKLLWVLDFPLF
                     EYAEDENRWVARHHPFTSPKPDHISVMINNNPVIENAEKYLEHPYNNIKANAYDMVLN
                     GNEIGGGSIRIYQRELQEKMFAALGMSSEEALHKFGFLLGAFEYGAPPHGGIAFGFDR
                     LCAILGGSESIRDFIAFPKNNSGRDVMLDAPGPIDEPQLNELSIQLNVRRDSL"
BASE COUNT         1205 a         1557 c         1501 g         1322 t
ORIGIN      
        1 aacatgaata aacacttccg tgtaaaggag tgtaaacact ttttcattga tattagattt
       61 agttttccga tggtttggat tccttgcttc aatagaaatt gaaagaaggg cttcccgtaa
      121 gaaacggcaa agcgctgtta aatcgtgtat tttccttgaa caattttatc cacaaaggac
      181 aaccggagag ataacccgtt tattatgtgg ctgtcagcaa catttcgata tgttcgattc
      241 catcttcgag atagatttcc cctttttgga caaagccgaa agattcgtag aagcgtaaca
      301 gatagtattg ggcgccgatg cggatatctg tctttccata tagcgaatac aacgtgtcga
      361 tactttgcac catcagctcg cgtccgacgc cgcggcgtct cgcggcaggg gaagttacga
      421 tcctgcctat cgaagattcc gcataggaga ttcccgcggg aacgattcgt gaatatccca
      481 cgagccgttc accccgccat cccagcaaat gatgacagta ttgatcctta tcatccatat
      541 ccaggaaaac gcagttctgt tctacgacga atacttcact gcgcaaccgc aacaccgcat
      601 acaattcatg cggagtgagc tgatcgaatt ctttcaaggt ccaggttgtc atatcgtata
      661 tcaataaccg tatttatcgc cccacaggct cttcaggaat tttcttaact cttcttcgcg
      721 cgcattctgg cccggctcat agaatttggt gccggccagc tcattcggca gatattcctg
      781 cgccgagaaa ttgcgctcgt aattatggct atattcgtaa cccttgccgt aatccatctt
      841 cttcatcaat tgcgtggggg cattacggat atgcagcggt acggacagat cgcctttcga
      901 ccggaccgcc tgctgcgccg ccccgatggc gacggtggcc gaattgcttt tgggcgagga
      961 ggcgagatag atggcgcatt gagaaaggat gagattcgat tccgggtacc ctatcttatt
     1021 caccgcctcg aagcaggcat tggcgagaag cagcgcgtta ggattggcgt taccgatgtc
     1081 ctcgctggcg agtatcagca tcctgcgggc gatgaatttc acgtcttcgc cgccttcgat
     1141 catccgggcc agccaatata cggcggcgtt aggatcgctg ccgcggatgc atttgatgaa
     1201 agccgatatg atgtcgtagt gctgctcgcc ttttttatcg taaagcgcga tccgttgctg
     1261 cgcgatctcc atgacgaggt cgtcggtgac ggttgccccc tttcctgctg cgtcggccac
     1321 tacttccagg aggttgagca gcttgcgggc gtcgccgccg gagagggtga taagggcttt
     1381 ggtttctttt agccgaatat ccaggctgcg gagccattcg tcgtcccgca gcgcattctc
     1441 cagcaatcgc ccgagatcct cttcactcag cggcttcagc acatacacct ggcagcggct
     1501 gagcaaggcg ctgttcactt cgaaagacgg gttttccgtg gtcgcgccga tcagcgtcac
     1561 gatgcccttt tctacggcgc ccagcaacgc gtcctgctgt cctttgttga atcggtgtat
     1621 ctcatcgata aaaagaatgg ccctggactt atcctttgcc tccgcgatga cttcccggac
     1681 ctcctttacg ccggcggaaa tagcgctaag ggtatagaag ggcaccttaa gggttcccgc
     1741 gatgatcccc gcgatggtgg tcttgcctgt ccccgggggc ccccaaagta tcatcgaggg
     1801 gggctttccg tgcccgatgg cggtccgcaa tacactacct ttaccggtga ggtgctgctg
     1861 accgaccagc tgatccagcg tttgcggccg tagccgctcg gcaagaggcg cttgtgaaga
     1921 cgacatatta aattttaaat aacaatagta caacatctta tcctttttcc tgtttaaagg
     1981 aagaggaggt aaaatagcac catgatggcg gggaatccag tatccatacg ccggttgact
     2041 acccttgtaa cctgtctcct gctggcgaac atctactgcc tcgccagcgg ccgggatcct
     2101 gttctctccg gggttcagaa ttatgcgctc aaccgaccgg gcgtcgtcat gatccgcacc
     2161 gaatataccg ccaacgtcta tgtcaacagg atgacgatgg atgaccggtc cttcaacgcc
     2221 ttgctggact cgattcaaaa gctggaccat ggcggaggcg tcagccccga gcaaaagctg
     2281 gatatcatgc tcagggagat gagtaaccgc ccatcccgct ttttccggtc gaccttcgaa
     2341 tatatcaaac agcccgagca gatcacctcc ataggtaccg gtttctttat caccgccgac
     2401 ggttatgtcg ctaccaattg tcacctcatc gaccgcgagg acgcttttat ccgtagacag
     2461 tttatcctgg ccgctttccg ccagatcacc gaagcaagca tcgccgcact ggagaattcc
     2521 tgggcaacgc attttaccga acagcagcgc acgctgctct acaataccta cgcttcggtg
     2581 tattcccgtc tgttcaccat cgtcctgtac gacctgaaaa agaacattta tgtggtctac
     2641 cggagcgatg acgcctaccg ccaatccgac acggtcaaga aagtggcgga tgtcatcgtc
     2701 aagggacagc cgatgccagg caaagatatc gcgatcctca agatcgagga cggtgggaat
     2761 ttgcccgtcc tcaggctcgc aggacccgcc atgccgcagg tgggcgagca gctttatgta
     2821 tacggctatc cgggaccggt taccaataat gactttgtat cggcggcctc ggctatcgaa
     2881 ccaaccctga ccacgggtat cgtcagcgcg atcaaacagt cggtgggaga ctggccggtg
     2941 atccagatgg atgccaatat caaccatggc agcagcgggg gacccgtatg caatgagaaa
     3001 ggagaggtgg tgggtctgac aacattcggt tccatcgaaa atacaggggg tctggcggcc
     3061 ggattgaact tcgcggtacc cgtgaccatc ctcgatgaat atgtcgacag cgcgggtatc
     3121 gatcccgagc cgggcacggc gacccttcat ttcaccagcg ctctcagcag ttatggccag
     3181 cgccggtaca gcgccgcgct cacggaattc ctggcggtgc agcggctgaa cccccattat
     3241 ccggggatct atgcctatat catggactgc caggaaatgg tcaaagacgg gaaggggcgc
     3301 cccatagggc cggtggaaag ggtgttattt atcggagccg ggcttctttt actcctgggc
     3361 ctgttctcct ggctcagggc gcgtcgggcg ggcaaatgag ccgcgcgatc aggatttatc
     3421 gctcctgatc cgatagtacc accgcaacag acgggtattc agcaccacat tcacgatgac
     3481 agccaatccc acgaccagcg atcccacgcc cagcagatgc aacaccccga tcagctgggc
     3541 atcgggtaat tttttcattt cgggcatgcc ttgcttgatg atctgcagcg gggtcttcgg
     3601 atcggtgagg aaaacgtagg cgttgatgac ggtatagatg ccgaagaata gcgcaaccag
     3661 tccgacaaag cggatgcccg taggcgtatg atggctcaat ttgacattgg ggtatcggat
     3721 ggccttgtgc agctgaagcg cggcatagtt gttcagcggt atggagcaga gcaggaacac
     3781 gcccgccaac gcgggataca actgtagggc cagcgccgac agcaacatca gccccagcaa
     3841 tccggtccag aagatcatgt tgaaccacgt cagtattcgc gcaatgatca aagtatttct
     3901 catagactat ctctcctgac attcagttgg atcgacagct cgttgagctg cggttcatcg
     3961 atagggccgg gcgcatccaa catcacgtca cggccgctgt tatttttagg gaaagcgata
     4021 aaatcgcgta tgctctcgct gccacccagg atggcgcaca gacggtcgaa accgaaggcg
     4081 atgcctccgt gcggcggtgc tccgtattcg aatgcgccca gcaggaagcc gaatttatgc
     4141 agcgcctctt cggacgacat acccagcgcg gcgaacatct tctcctgcag ctcacgctga
     4201 tagatccgga tcgacccgcc gccgatctcg ttcccgttga gcaccatgtc gtaggcgttg
     4261 gccttgatat tattgtaggg atgctcgaga tatttctccg cattttcgat gaccgggttg
     4321 ttgttgatca tgacggaaat gtggtccggt ttcggcgagg tgaaagggtg gtgacgggct
     4381 acccagcgat tctcgtcctc ggcatattcg aacagcggga aatcgaggac ccagagaagt
     4441 ttgaactcgt ccggcttgcg cagccccagc cggcggccca tttcgagcct cagctcactg
     4501 atcgctttac gcgttttctc ctcgcggccc gcaaggatga ggatgaggtc gccgggttgc
     4561 gcgtttgcgg cctccgcgat ggccttcagc ttcggctcgg gatagaattt atcgacgctg
     4621 cttttgaagc tgccgtccat attgcatttg atatagacca ggccctgcat cccgatctgg
     4681 ggacgcttga cccattccgt tagctcgtcg gtttgcttgc gggtatactc ggcacagccg
     4741 ggtacggcaa tggcgagaac ggtctccgca ttgtcgaaga cgccgaaacc tgcgccggcg
     4801 atgagttcgt cttttcctcc cttcttaaag gctgtcttca aattcaacag cttcatcccg
     4861 aaccggatat cgggcttgtc attaccgtat tgccacatcg cctcctccca ggtcatcctt
     4921 tctatctttc cagcataatc gatatctttt acttctttaa agatatgccg caccatcccc
     4981 tcgaacatgg acaggatatc ttcctgttcg acaaagctca tctcacagtc tatctgggtg
     5041 aactcgggtt gacggtctgc acgaagatcc tcgtcgcgaa aacagcgtac gatctggtaa
     5101 taccggtcat aaccgctgac catcaacagc tgtttgaagg tttgcgggga ctggggcagg
     5161 gcatagaact gccccggatt catccgcgac ggtacgacga aatcgcgggc gccttccggc
     5221 gtggatttga tcaggaacgg ggtctcgagg tccatgaatc cgttggcgtg caggtattcc
     5281 cgcgtggcgc ggttcacggc gtatctcagc tcgaggttct ttttcacggt cggccggcgc
     5341 aggtccagga accggtattt catccggaga tcgtcgccgc cgtccgtctc gtcctgtatg
     5401 gtaaaaggag gagttgccgc cttgttcagg atcctaaagg agctgacctt gatctcgatg
     5461 tccccggtgg gcagctgggg gttcctgctg gctctttcgg ccacctcgcc cgtcacctgt
     5521 atgacgaatt cccgccccag gggttgacga tccagctctt tattcaaatt ttcctgctct
     5581 ccgaa
//
LOCUS       DASYSV010000365         7340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_969883, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000365 DASYSV010000000
VERSION     DASYSV010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969883"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VGQ51_11175"
     CDS             <1..1100
                     /locus_tag="VGQ51_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV9037178.1"
                     /translation="NINTKRQVGSAIKPFLYSLGIEDYGLTPESECETSQQYFPGFGY
                     VPARPDKHEGGSMTMASGLAWSINGVAAYIMKQVGPKRFAEYMHQIGIPTKIEPYPSM
                     ALGACDLSLYEMMWGYTMFPSGGFSTRPYYISRIEDKNGNVLQRFDTERKEVISQGTA
                     YTMARMMQGPVDFGTAAGLRNRLGISEMGGKTGTTNDNSDAWFMGYTPQLMAGAWIGC
                     DDRFIHLESGLGYGAQAARPIWEYFFAKVLADKSLGIERQAKFIPPENVRKEMMYDYM
                     NIEKIAPPGAEGANMGNGKANQYLDTSPPTVPVDSRLSPEEQQVLKEATHEKSSKEGS
                     VKITVTDANKNQPPPKKKGSFFKRLFGKHDR"
     gene            complement(1181..1654)
                     /locus_tag="VGQ51_11180"
     CDS             complement(1181..1654)
                     /locus_tag="VGQ51_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV9037179.1"
                     /translation="MKASIGIKPADLQAVSTELSKLLADEFILYTKTRNAHWNVESPD
                     FHAMHIFFEQQYEALDEVMDSVAERIRQLGHYSPGTLASFLSLTHLTEKTGQRNDSMD
                     FIRELLTDHENIIIFIRENINKFADKYGDAGTSDFITGLMEEHEKMAWMLRAHLK"
     gene            complement(1861..2301)
                     /locus_tag="VGQ51_11185"
     CDS             complement(1861..2301)
                     /locus_tag="VGQ51_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEV9037180.1"
                     /translation="MSTKKLRAGLFACVLLPVAVLGQHHHRPAPVKVQYGVASFYSDK
                     FEGRKTYTDEIFHQDSLTGASNTLPMHTWIRVTNLRNHKTLVVRINDKMHPRNKRLID
                     LSRAAAQRLGYSGRGLTKVRIDVLGKKRPPDAVEDLADRRPGQD"
     gene            complement(2343..4025)
                     /locus_tag="VGQ51_11190"
     CDS             complement(2343..4025)
                     /locus_tag="VGQ51_11190"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV9037181.1"
                     /translation="MNYPERRLFLVLFSLISTLFFCCHGKPGGQAVTDHVENPKKMDE
                     HVGTDLKSVLQYAAANDDRMNDKTTLQYRKLEDSLYEVNGYTPIWSDKERWVPLGDSL
                     LAFIRDSKDYGLFPSDYHWDALSFIQRIVEEDSNARKNAALWTRGDLLLTDAFFTLVK
                     DLRNGRMDFDSVTLRKDSVMSDSLFTTTLASARGLGAIAPVLDSLEPRHPGYDSLKRY
                     LRDFLATAHFRQNTWLDYPYKDSAVFYNALGHRLQESGYIDSGVTQFDTAMLASAMRR
                     YQTINKLKVRGKPTSEMVNMMNNTDWEKFKRIAINMDRYKHLPDTLPKTYVWVDLPAY
                     FLQVVDSDTVVFESRVIVGSPKTRTPLLTSEISNFVTYPQWTVPMSIIMKEMLPAIQK
                     NVNYLAKQNLMVVDDNDSVRDPTKINWRRLNKHNFPFQLKQRQGDDNSLGVMKFNFRN
                     KYDVYLHDTNVRWMFDERYRAVSHGCVRVKDWAKLSAFLIRKDTMKYHPDTLKAWIAR
                     QEKHTVYGFAKVPIFIRYYTVVGRKGKLAFYDDIYGEDRELRNRYFAKKPIY"
     gene            4268..5029
                     /locus_tag="VGQ51_11195"
     CDS             4268..5029
                     /locus_tag="VGQ51_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9037182.1"
                     /translation="MKSGPDSVAVQQKPEPLLQLNLSNGEYNLIYSPGFLSSPWLTFM
                     GKNGIVISLGVRIDHLLHLGHTIQSLPSFLAKVQKGMACKLTNKPAMADLKMIRAARD
                     MMDCHFTGTVKAVYCELKLSNLLVMALAGISGQANADKMVLKPLDVEKIEATRRYLFD
                     NMDHPPTLIRLARKMGINDYKLKKAYKQLYGTTLFEDFLHARMDKAKTLLQDTSQSIV
                     GIAETVGYKNVSSFALAFKKYFGCPPGEYRIKPYI"
     gene            5026..6024
                     /locus_tag="VGQ51_11200"
     CDS             5026..6024
                     /locus_tag="VGQ51_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9037183.1"
                     /translation="MKSPIEIAGEKTGTIHLSPALRTMYGPQHIPGLRCLEARGAFGH
                     LLFQEIQGDDFSVWHCSFYLNHDDKLTLTWSLPVLRLWMALKCSFYFTSDNTGENVLH
                     ERGFRFDYFLTPCQTIRLRGKQSYSCVAVHFSHDFAREMAQCIPGGELFLQNVEKGEQ
                     TSPFPYVAGMDMLGAVEDILYCTYTSEFRRQYLSTKTLELLFLALERSTRARLSDSGR
                     LPELVVRRIYEAAQLITRHLARDYSLRDLGVLVGLSDYQLKRGFKTIYNLTAADYRHE
                     IRMKKARLLLEETDRPIAGIASEVGYDHPFAFSSAFRKFFGCPPSIVRKGKRIRTN"
     gene            complement(5993..6577)
                     /locus_tag="VGQ51_11205"
     CDS             complement(5993..6577)
                     /locus_tag="VGQ51_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV9037184.1"
                     /translation="MPSDRLCKPIDPYRQQHPVFSRPYPLQEYTLCIRPLCVSTDIEL
                     VCSWLSHQLGTHSWQEGGPKPELMQCYIDMLKSDYSQSFLCLMDEQPVCQVDVGKAIF
                     NEVFMYIDTQDNDYVFRMIISPEVKLRNAYVNIVRTLLEYFFSFREVGRVLTCLPAND
                     DWINHLLESAGLAYLDTRRTIYGVVNLYGCVSPS"
     gene            6857..>7340
                     /locus_tag="VGQ51_11210"
     CDS             6857..>7340
                     /locus_tag="VGQ51_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037185.1"
                     /translation="MRTTLSVILFLLISCSTLHGYGQQQPPPKQNDTMLLKIDSLRAA
                     VVTATMRPRMKGDTLVYNTEHILLRKNALVEELLRRLPGLQINPDGTITYNGDKIEHL
                     LVDGEDIFGSDPTTVTRNFDASKIARVEILDRKSDQAIFTGIDDGTRTRTLNLVLKPT
                     A"
BASE COUNT         1822 a         2093 c         1734 g         1691 t
ORIGIN      
        1 tcaatatcaa caccaaacgg caggtcggca gcgccatcaa acccttcctc tattccctcg
       61 gcatagaaga ttatggactc acacccgaat cggaatgcga gaccagccag caatactttc
      121 cgggctttgg ctacgtcccc gcccgaccgg ataaacacga aggcggcagc atgaccatgg
      181 catccggcct ggcctggtcg atcaatggcg tggccgcata tatcatgaaa caagtaggcc
      241 cgaaaaggtt tgccgaatac atgcaccaga ttggtatccc tacgaagatc gaaccctatc
      301 cttcgatggc cctcggcgcc tgcgacctct ccttatacga aatgatgtgg ggctatacca
      361 tgttccccag cggcggcttc agtacccggc cgtattatat ctcccgcatc gaagataaga
      421 acggcaacgt cctccagcgt tttgacaccg aacgcaaaga ggtgatcagc cagggcacag
      481 cctataccat ggcccgcatg atgcagggtc cagtggactt cggcaccgcc gccggcctca
      541 gaaatcggtt aggcatctcc gaaatggggg gtaagacagg caccaccaac gataattccg
      601 acgcctggtt catgggctat acgccgcaat tgatggccgg cgcatggata ggttgcgacg
      661 accgcttcat acacctcgag agcggacttg gttatggcgc ccaggcggcg cgccccatct
      721 gggaatactt ttttgccaaa gttcttgcag ataaatccct cggcatcgaa cgccaggcca
      781 agttcatccc gccggaaaat gtgcgcaagg aaatgatgta cgactacatg aacatcgaaa
      841 agatcgcgcc ccccggtgcc gaaggcgcca atatgggcaa cggaaaggcc aatcaatacc
      901 tcgatacgag cccgccgacc gtgcccgtgg attcgcgtct gtcccccgaa gaacagcagg
      961 tcctgaaaga ggcgacgcac gaaaaatcca gcaaggaagg ctcggtaaaa ataacggtga
     1021 ccgacgctaa taaaaatcag ccgcccccca aaaagaaggg cagcttcttc aaacgccttt
     1081 ttggcaagca cgaccggtaa ccatcactgc tccgcgcccg acctccacgc acgaccgatg
     1141 atcatcactg ctccgccccg acctccacac acccccggcc tcacttcaga tgcgcgcgca
     1201 gcatccaggc catcttctcg tgctcttcca tgagcccggt aataaaatca ctcgtcccgg
     1261 catcgccata cttatcggca aacttgttga tattctcgcg gataaaaatg atgatgttct
     1321 catggtcagt cagcagctca cgaataaaat ccatgctgtc attgcgctga ccggtcttct
     1381 ccgtcagatg cgtcagcgaa agaaaacttg ccaatgtccc gggcgaataa tggcccagct
     1441 gtctgatccg ctcggccacg gaatccatca cctcgtccaa cgcctcatac tgctgctcga
     1501 aaaagatatg catggcgtgg aaatcgggac tctccacatt ccaatgtgca ttgcgtgtct
     1561 tggtgtacag gataaactca tccgcgagca atttgctcaa ctccgtggac acggcctgca
     1621 gatccgcagg cttgatgcca atgcttgcct tcataataga tagtttttac gttcaggaac
     1681 aatcgacaga ctcatcctgt tcacaaccgg acagcaaaac gtccagttcc ggacaccgct
     1741 cgcccgtccc cggatagcaa ctgcattaat tatcatgcca ttatacgcga aaggagattt
     1801 ggcatgccct gtgccctccc gccagggtag taacgtttta gttttttaga aggtgaagtg
     1861 tcaatcctgc cctggccgcc ggtcagccag atcctcaacg gcatccggcg gccttttttt
     1921 gcccagaacg tcgatgcgca ccttggtaag gccccttccg gaatagccta agcgctgcgc
     1981 cgctgcgcgg ctcaggtcga tgagacgctt gttacgcgga tgcatcttgt cattgatccg
     2041 gacgacaaga gtcttatgat tgcggagatt ggtaaccctg atccaggtat gcatcggaag
     2101 cgtattgctg gcccccgtca ggctatcctg gtggaagatc tcgtcagtat acgtcttcct
     2161 tccctcgaat ttatcggaat agaaactcgc gaccccatat tgcaccttca caggcgcggg
     2221 gcgatgatgg tgctggccaa gcacagccac cggtaacagt acacaggcga aaagcccggc
     2281 tcttaatttt ttcgtggaca tggattattg gatttgggcg acagccgccg atccacaaga
     2341 tatcaataaa tcggcttttt tgcaaaatac ctgttcctca gctccctatc ctccccgtaa
     2401 atatcatcat agaacgccag cttccccttc ctccccacca ccgtataata acggataaag
     2461 atcggcacct tcgcaaaccc atataccgta tgcttctcct gccgcgctat ccacgccttc
     2521 agggtatccg gatgatattt catcgtatcc ttgcgtatca ggaatgccga tagcttcgcc
     2581 cagtccttca cccgcacgca cccatgactc accgcccggt acctctcatc gaacatccaa
     2641 cgcacattgg tatcgtgcag gtatacgtca tacttgttcc tgaaattgaa tttcatgacc
     2701 cccagcgaat tatcgtctcc ctgtcgctgt ttcagctgga aggggaaatt gtgcttattc
     2761 agccgcctcc agttgatctt cgtcggatcc cgcacactgt cattatcatc cacgaccatc
     2821 aggttctgct tcgccagata gttgacgttc ttttggatcg ccggaagcat ttctttcata
     2881 atgatgctca tcggcaccgt ccattgcgga taggtcacga agttcgagat ctcgctcgtc
     2941 agcagtggcg ttctcgtctt cggcgagccg acgatcaccc tggattcaaa aaccaccgta
     3001 tcggagtcga ccacctgcag aaaataagcc ggcagatcta cccagacata ggtcttcggc
     3061 agcgtatccg gcagatgttt gtaacggtcc atgttgatgg cgatacgctt gaatttctcc
     3121 caatccgtat tgttcatcat gttcaccatc tctgaggtgg gcttccccct taccttcagt
     3181 ttattgattg tctgataccg gcgcatcgcc gaggccagca tggcggtatc gaattgcgtc
     3241 acccccgagt cgatataacc cgattcctgc aaccggtggc ccagcgcgtt gtaaaacacg
     3301 gcggaatcct tatacggata atccagccac gtattctgac ggaaatgggc agtcgccaga
     3361 aaatcccgga gatatcgctt caacgaatca taccccggat gtctgggctc caggctgtcc
     3421 agcaccggcg caatagcccc gagaccacgc gcagaagcca gcgtcgtggt aaacaggctg
     3481 tccgacataa ccgagtcttt ccgcaacgtg acactgtcga aatccatccg gccgttgcgc
     3541 aggtccttca ccaatgtgaa aaaagcatcc gtcagcagca ggtcgccccg cgtccaaaga
     3601 gccgcattct tccgggcatt cgaatcctcc tctacgatac gctgtatgaa cgaaagcgcg
     3661 tcccagtgat agtcggatgg gaatagcccg tagtccttgc tgtcccggat aaaggctagc
     3721 agactgtcgc caaggggaac ccaccgctcc ttatcgctcc aaataggagt atacccattt
     3781 acctcataca gactgtcctc cagcttccgg tattgcaggg tggtcttatc gttcatccga
     3841 tcgtcattgg ccgccgcata ctgcagtacg gatttcaggt cggttcccac atgctcatcc
     3901 atctttttgg ggttttcgac atggtccgtc accgcctggc cgccgggttt gccgtgacag
     3961 caaaaaaata gcgttgaaat gagggaaaaa agtactaaga aaagacgacg ctcggggtag
     4021 ttcatttgac ggtaagatac aacgattgat tagcttatca acgcgtataa catttttttt
     4081 tactatttat tcacatgacc ttatccattt cttcacagac cggtatgccc cgccggagca
     4141 tatcccgacc aggttattaa cgatccgaat cgtggcaaat tgtttgcatt gatactaatg
     4201 ccgtctaaat aatatcctat cgctttactc taatcttatc cttaaaacaa tgctccgtca
     4261 acctatcatg aaaagtggcc cggattccgt ggccgttcag cagaaacctg agccgctgtt
     4321 gcagctcaat ttatccaacg gcgagtacaa tctgatctat tcgcccggat tcctttcctc
     4381 gccctggctg acgtttatgg gtaagaacgg catcgtcatc tcgctgggag ttcggatcga
     4441 ccacctcctc catcttggac atacgattca aagcctgcct tccttcctgg caaaagtgca
     4501 aaagggcatg gcctgcaagc tgaccaataa accggcaatg gccgatctta aaatgatccg
     4561 cgccgcaagg gacatgatgg attgccactt taccggtacc gtcaaagcag tctactgcga
     4621 gctgaaactt tccaatctgc tggtcatggc gctggcaggc atcagcggac aggcaaacgc
     4681 ggacaaaatg gtcctgaaac ctctcgatgt cgaaaagatc gaagcgacac gccgctatct
     4741 tttcgacaac atggatcatc cccctacact gatcaggctg gcccgcaaaa tgggcatcaa
     4801 cgattacaaa ctgaaaaagg cctataagca attatatggc actacgctat tcgaggactt
     4861 tctgcacgcc cgcatggata aggcgaaaac attgttacag gacaccagcc aatccattgt
     4921 aggcatcgct gagaccgtcg gctataaaaa tgtttccagc ttcgcccttg ccttcaagaa
     4981 atatttcggt tgccccccgg gggaatatcg catcaaacct tacatatgaa aagccccata
     5041 gagatcgcgg gcgagaagac aggcacgatc cacctgtctc ctgctctgcg gactatgtac
     5101 ggaccgcaac acattccggg cctccgttgt ctcgaagcca gaggagcatt cggacacttg
     5161 ctgttccagg aaatacaggg agacgatttt tccgtttggc attgcagctt ctatctcaac
     5221 catgacgata agctgaccct gacatggagc ctccctgttc tccgcctgtg gatggcgctg
     5281 aagtgcagct tctattttac ttcggacaac accggcgaaa acgtcctgca cgaacgggga
     5341 ttccggttcg actatttttt gaccccgtgc cagacgatcc gcttgcgggg aaaacaatcg
     5401 tattcctgtg tagcggtcca tttttctcat gattttgccc gggagatggc acaatgcatt
     5461 cccggcgggg agttatttct acaaaatgta gaaaaaggag aacaaacttc cccctttcca
     5521 tacgtagccg ggatggatat gctcggtgct gtagaggata tactttactg cacctacacg
     5581 tcagagttta gaagacaata cctcagcacg aagacattgg agttgctttt tctcgccctc
     5641 gaacgcagca cccgcgcacg cctctccgat tccggtcggc tcccggaact ggtcgtcaga
     5701 aggatctacg aagccgctca actgatcacc cgtcacctgg cccgcgatta ttctctgcgg
     5761 gatctcggcg tattggtggg actcagcgac tatcagttga aaagagggtt caagacaatt
     5821 tacaatctca ccgccgccga ctaccggcac gagatcagga tgaaaaaagc cagactcctg
     5881 ctcgaagaga ccgataggcc gatcgccggc atagcatcgg aggtcggcta tgaccacccc
     5941 ttcgcctttt catcggcatt ccgcaaattt ttcggctgcc cgccatcgat agtcaggaag
     6001 gggaaacgca tccgtacaaa ttgaccacgc catagatggt gcgtctggta tccaggtagg
     6061 caagccccgc gctttccagc aggtgattga tccaatcgtc attggcgggg agacaggtca
     6121 gcacccgccc gacttccctg aaggaaaaga aatattcaag cagcgtccgg acgatattga
     6181 cataggcatt tcgcagcttc acctccggcg aaatgatcat ccggaaaaca taatcgttat
     6241 cctgcgtgtc gatgtacatg aacacttcat tgaagatggc cttgcctaca tccacctggc
     6301 acaccggctg ttcatccata agacagagaa aggattgcga ataatccgat tttagcatgt
     6361 ctatataaca ttgcatcaat tccggtttcg gaccgccctc ctgccacgaa tgggtgccga
     6421 gctgatggct cagccagcta cagacgagtt ctatatccgt tgaaacacac aacggacgaa
     6481 tacacagggt atactcctgc agaggataag gtctggaaaa taccggatgt tgctgtcgat
     6541 aaggatcgat aggcttgcac aggcgatcgg agggcataat ggagcaatgc cgcaaataaa
     6601 aggccctctt ttgtaactat tccgctttgg tttggtttta atccctgatt cataaacagc
     6661 aggctccgcc gggtgaatac ctttgttctg atgatagcaa ctaaccccgg ttcgcgaaaa
     6721 gtcccattac cgatatccca tccattacgt tagaccttat cctacacatg aaaaggcgag
     6781 atccctgatg tgcttttgct tgtcggggat cccctttttg gtccaaaaag ccgaaaattc
     6841 ccttattctt tcctctatgc gtacaacctt atctgttatc ctctttttat tgatctcctg
     6901 cagtacgcta cacggctatg gccaacaaca accacctccg aaacagaacg atacgatgct
     6961 tctcaaaatc gatagtctcc gggcggcggt cgtaaccgcg acgatgcgac cgagaatgaa
     7021 aggagatacc ctggtatata atacggagca tatattgttg cgtaaaaacg cgctggttga
     7081 agaattgttg agacgcctcc ccggcctgca aataaacccc gacggcacca ttacctataa
     7141 tggcgataaa attgagcatc tgttggtgga cggtgaagat atattcggat cggatccgac
     7201 caccgttacc cgtaatttcg atgcaagcaa gatcgcacgg gtagagatcc tggatagaaa
     7261 gagcgaccag gcaattttca ccggcatcga tgatggcacc cggaccagga cgttgaacct
     7321 ggtattgaag ccgacggcga
//
LOCUS       DASYSV010000366        17451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1096403, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000366 DASYSV010000000
VERSION     DASYSV010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 17451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17451
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096403"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VGQ51_11215"
     CDS             <1..391
                     /locus_tag="VGQ51_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037186.1"
                     /translation="YFSPYFLWQQAMNHDFSGDAYYRFSIEVGFLECLLKGGFVLFLL
                     YITPMVAAIRRGLFSRHNNKVAYLIAIYVLVELLILFVENIPSWHFQFFLIFFLAGYC
                     CRQTALQQSPANENFRYNALLQPGSLY"
     gene            342..1199
                     /locus_tag="VGQ51_11220"
     CDS             342..1199
                     /locus_tag="VGQ51_11220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9037187.1"
                     /translation="MKISVITPSFNQGRFIEDAIRSVLIQDYPDFEHIVIDNCSWDNT
                     LDVLKKYPHIRWISEPDHGQSHALNKGFEMATGDILAWLNCDDFYLRDAFHTAARVLG
                     DPNIDGIYSDLQFCDLNWRITKHYQSHRPVKFLSLFHNFISSECFFFKRKIIDNQIHV
                     REDLHYCMDQDFAANILHHNYRLHYMKACFAVFRWHGANKSVDTPWRRDQRTREGIRI
                     FNRHNGYIRLDEDNPNHRKLYSWGRTLLKPYRLFLKATNNIDDKIHHHKLGRWPSFGR
                     APRRSAGQI"
     gene            1120..1842
                     /locus_tag="VGQ51_11225"
     CDS             1120..1842
                     /locus_tag="VGQ51_11225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV9037188.1"
                     /translation="MTRYITTSWDDGHPSDARLAALLAKYDLKATFYIPKANPGHRVM
                     KEEDILGLSRHFEIGGHTLRHLSLKELSHYAAWEEIQGSYEWLRSLLGKSPMSFCPPF
                     GEYTARHLSLIYETGFKVVRSTELLSTRPSSGAIPTTLQVYDHSRFTYFKHLLKRGRL
                     QNLGLWCGSGFSSDVFRLLDHYLEHLMAHGGCLHLWGHSWEIDHYRQWKRLEKIFSVI
                     SHLSDFSYVENGQLSKTNSPVI"
     gene            1839..3059
                     /locus_tag="VGQ51_11230"
     CDS             1839..3059
                     /locus_tag="VGQ51_11230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV9037189.1"
                     /translation="MKVLCIHTYYMQRGGEDAVFENELRLLSDAGCETLPLLFNNRRF
                     AALKFLFFFFNPLSFWKTCRAIRRWRPDIVHVHNWFFSASPAVFLAARCMGVPVVHTV
                     HNFRILCPSSFLFVRNELFTDCMTRIFPLRAITRKIYRGSALSTAWLLIGTRLHYFLR
                     TWQKIDLLICLTDPARQMICQSLLKIDPHRIAIKPNFFKVEELPVPQGMVPARRCGFL
                     FVGRLSPEKGIQLLLDSFRNSPYTLRIVGDGPLRAEVEACAAEYSNIQYLGLQTKQQI
                     IGHLRSAEALVFTTIGFEQFGLVIIEAFSCGTPVIGPDTGSPAELIIEGYNGLHFKTG
                     SVPDLRRKIRYWHSLPKEVREVYSENSADTYGRYYTPEINLRLLSNIYKKVLHENNAN
                     LQLPHQRREVPGLH"
     gene            2998..3774
                     /locus_tag="VGQ51_11235"
     CDS             2998..3774
                     /locus_tag="VGQ51_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV9037190.1"
                     /translation="MKTMQIFNFPISVGKYQDYIEYIMDSASTGRSQYIYVANVHMVV
                     EAFWNKSFSEKVRKAGLVTPDGKPLTWALRLLHGIKQDRVAGMDVLPALLKQSEKSQT
                     PVYIYGGTETLLSRTRNYLDRNYPKLCIKGLYSPPFRDLTPEEEARTIERINSSGAKL
                     VFVVLGCPKQEQWMASMAGRINAVMIGIGGALPVLIGMQKRAPRWMQNMGLEWLFRLW
                     LEPRRLFKRYFVTNSVFLYLLSRELVKKTFVRKFEPHEMN"
     gene            3781..4935
                     /locus_tag="VGQ51_11240"
     CDS             3781..4935
                     /locus_tag="VGQ51_11240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9037191.1"
                     /translation="MKTLICDLNVYDNGHHIAFVNSILSHTARREDLIFLFNGMAATL
                     CKGMEGNDRIFFVPEDNLHPDELGSLRDKMREYRFIRNFALEHGVERVIFLEIDWYQV
                     AIGLSSRPPFRITGIYFRPFHLIPTRGDSLRDSVKRLLYHAKKRFLFHILKFNRKVEP
                     LFLLNDRMAGVRYPSWFRYLPDPVFGGGAGPGGGGSVTIRRQFDIPSSSHIILAFGAM
                     GARKNIGNIVAAYQQANFEGDTVLLIAGKVRADYRGAFNRAIDSFGCNNDGRKRLIVF
                     DTFVDEEHIDLYFSEAGTILLCYSKFYGSSGLMGKAAQHRKTCIVPDCGLLYDLNREY
                     KLGYSVDPENVDSIANALSLAQRAPVEEAGFQRFVRDHHESAFLRTLLTV"
     gene            4948..6342
                     /locus_tag="VGQ51_11245"
     CDS             4948..6342
                     /locus_tag="VGQ51_11245"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEV9037192.1"
                     /translation="MNNRFVNHLQVTLIVLDLAVINFIFFFSQFLFRRSLAITAVLEY
                     DYFLYFMNICWILTTSLTGVYNSKTIVNFETFSRRSMNAFLYFTGFVTLYLYFFHQII
                     ISRMFIVAQLVGIAVLMLANRFIFLGIYQYFRKKDHLLRKIVVIGYNNVAKKLVQHLE
                     NQPFNSRVIGFCEDTENVREVTHYPILSNIRGVLEECERQGVTEIYSTIAPEQNAYLY
                     ELIRGAERQCIRFRLVPDLGVFMMKKQVYIDYLEDMPVLSLRREPLEDLGNQIRRRIF
                     DIIVSSLVVIFVLSWMVPLVGLAILLDSRGPIFFKQARSGKNNRSFPCLKFRSMVKNN
                     TTEHRQATRNDSRVTRIGRFLRRTSIDEFPQFLNVLKGDMSIVGPRPHMLKHTSDYSQ
                     QLDHFMIRQFVKPGITGWAQVHGYRGETRDLGQMEKRIEHDIWYLENWSLWLDVRIVF
                     MTVFNLFRREANAY"
     gene            6444..8243
                     /locus_tag="VGQ51_11250"
     CDS             6444..8243
                     /locus_tag="VGQ51_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9037193.1"
                     /translation="MSEKRQKKAVQAGPGGKPMGPGGKPAGPGLFSILKPYRQWIILL
                     VVLSLVSNGINLLIPNISKNAIDAFIAHRLDVDRTLLLFLSAIVGVFVFTYASSFVQT
                     YTSERVARDLRTRLADRISRQNYSFLQKKNTGQLLTNLTSDVDSIKGFVAQAIVSIFS
                     SIFIIIGASIFLLTIRWQLALCVIAIIPIIGFTFFIVLKKVRVLFKASREVIDTLNKV
                     INESIIGAALIRVIHSQHLEYTKFLEANAKALGLGLRILKLFATLIPIIIFTANLATL
                     TILAFGGHYVIAGRMTIGQFTAFNMYLTMLIFPILVIGFMSNVIAQAQASYGRIAGVL
                     YTPDPEEGGTRVSDLKGDIDFKDVSVSYGEKMVLKDISFHIHGGSKTAVIGPTAAGKT
                     QLLYMLTSLSHPTRGTIEFDGYPLDGYRKDDFHRQVGFVFQDSILFNLSIRENIAFSD
                     NVSDESLKKAIATAELTAFIDELPEGIDTVVSERGTTLSGGQKQRIMLARALALDPRV
                     LLLDDFTARVDNQTEKKILENVRANYPGITLLSVTQKIASIEDFEQIILLMEGEMIAM
                     GTHAQLMQTCPEYVQIYQSQKSTSHYEQPELQS"
     gene            8218..10107
                     /locus_tag="VGQ51_11255"
     CDS             8218..10107
                     /locus_tag="VGQ51_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9037194.1"
                     /translation="MNSRNYNLNELTAGRSKSKIWPALTKLVGLLPEQRGKLIGALLI
                     MVVYSILSMLPPALIGYTINHLIEGKINTVSTGIPDQSTWMTHLFHGSGYSLIVTVCC
                     CLIGIYLLNLGAVYWRTILMGGFGQHLLYTLRNAVFNKLQELPVAFFAQNKAGDLISR
                     INNDTDKVNQFFSQSLMQFISSIFIIAGAGVALVYFNWRLGLLTLSPAALMWLFNKVM
                     SKWVKKKNAASAKATGGLSAEIQESLNNFKVVLAFNRRDYFRKRFGQANTANYSSAVG
                     AGIANSVFNPVYSLLSGLGQMVVLFYGIYLIRTGYFTPGFLVAYFMYTSSFYDPLRQL
                     AALWAGFQVAIASWDRISHILSLDTDLVTIHESDIPAIPPGGLSVVRTGNGASAGQPP
                     LIEFREVSFAYPGGKDVLRHNSFRMEKGKTYALVGPTGGGKTTTASLLARLYDPTAGA
                     VILHGRDIRTYTPAERAEKIGFILQDPILFSGTVRENILYGNEKYKDGPNEQLTGVLH
                     SSGLETLLTRFDDGLDTAVTLGGEGISLGQKQLIAFIRAVLREPELLILDEATANIDT
                     VTEKMLEDILRRLPPTTTRVIIAHRLNTIAGADEIFFVNAGEITRAGSLDQAVDMLME
                     GKRVS"
     gene            10232..11233
                     /locus_tag="VGQ51_11260"
     CDS             10232..11233
                     /locus_tag="VGQ51_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEV9037195.1"
                     /translation="MQRLLTCSFASLALCLAVHTANAQSGYAVTGTFRIASPGGWDYI
                     SVGPDKKVYVSHGTQVNILDQKTGDSVGVIPNTTGVHGIAFDEHHGKGYTSNGRLNNV
                     TVFDMKTDAIEGQIATGQNPDAIMYEPFTKTIITCNGRSHNLSIIDCASGRVTDSIAL
                     DGKPETAVSDGEGMVYVNIEDKNEISEVDMRKKTVVASWPLGAESPTGLAIDLKTKRL
                     FAGCEKQLVVMDATNGSVVAKLPIGNGCDGVGFDEGLKLVFASCGEGVVTVIRESSAS
                     SFSVIENVPTKRSARTCAVDSRTHSVYLPAADTEPAQAGQRPRMIPGTFQVLVVSRK"
     gene            11263..12705
                     /locus_tag="VGQ51_11265"
     CDS             11263..12705
                     /locus_tag="VGQ51_11265"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV9037196.1"
                     /translation="MQNFINGGFAGAGSGRSLSVLSPIDGRALAEMPCSDRNDLDDAV
                     EAARAAFPAWSRMPVKERVQVFFRYKRLLERDMDTLAALVSEENGKTMSEAIAEIEKC
                     IELTEFATSLPQLIGGEILEVSKGVECRLDHVPLGVVASIVPFNFPAMVPHWTIPNAL
                     ALGNCMIVKPSEKVPLSCARTALLLREAGLPDGVFNIVNGDSAIVEAICDHPGIDAVS
                     FVGSTKVAKIVYRRATQHLKRCLALGGAKNHLLVLPDAQPSMTAANVVASMSGCAGQR
                     CMAASAMVAVGPVDDIIGQICEGARKIIPGQNLGAVIDRTAKERIERYITEAERQGAR
                     ILVDGRGYTVPGKEGGSYVGPTVIDHVRPDMAIASEEIFGPVICIMRVDTLDEALAIE
                     NASPYGNAASVFTQNGSYARYVIERASAGMVGVNIGVPVPREPFSFGGWNDSKFGVCD
                     ITGKSSIGFWTKLRKSTVKWNPEAGINWMT"
     gene            12727..14082
                     /locus_tag="VGQ51_11270"
     CDS             12727..14082
                     /locus_tag="VGQ51_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV9037197.1"
                     /translation="MPASNTLSETQEIVQDNLDYTLFSWSKQHGINPIAVQRAEGVYL
                     YDYDGKRYLDFSSGLMNVNIGHGNQRVTDAVVRQMQEVSYVTPSCVTRARGELGKKLA
                     EITPGRLTKTLFTVCGATAIENALKVARLYTGKHKVIARYRAYHGSSYGAMTAGGDPR
                     KLQTDAQQAPGMIHVEDPYCYRCPWNQEMSSCNRECVSHIQRIIEFEGPENIAAILME
                     GESGSSGCIKYPPDYLKKVRALCDRYGILLIADEVMSGFGRTGKWFACDVHGVVPDMI
                     ATAKGITAGYLPMGALIVSDAIARYFDDRVLWTGLTYSAHPVACAAAVEVLKIYEDEN
                     LIENAARMGRYLDEQAERLRAEHPCIGDFRNTGLLGVFEIVKNRSTKEPMAPFNAKPD
                     EMAVMNKVAARLKEVGMYTFVRWNYLFVAPPLCITRDQIDEGLAMIGDALTIADAYVT
                     E"
     gene            14120..15265
                     /locus_tag="VGQ51_11275"
     CDS             14120..15265
                     /locus_tag="VGQ51_11275"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV9037198.1"
                     /translation="MTATAQRARDYGVPFDGHPGAFNAITDVPGVTVGQTTIISGEGP
                     LVKGKGPVRTGVTAILPRGPKFGPVYANWYTLNGNGDMTGTHWITESGFLETPVLITN
                     TGSVGTVRDAAWQWMDQHHYYAPFYKDYWYAYPVVAETYDGIFNDINGQHVTKADVFH
                     ALDSARSGLVEEGGVGGGTGMMCYNFKGGIGTASRVLDARDGGYTVGVLVQCNHGSRG
                     QLTIAGVPVGRRLQDTLPYIIEGQSFGVTDALPRDETGSIIVILATDAPLLPGQLKRL
                     AQRIPLGVSRTGGIGGNGSGDIFLAFSTANPQAFSNTENREVTTLPNDRINPLFEATV
                     EATEEAIVNALFAGKTLVGINGNTVPGLPKEAVIRILREYNRLAPGH"
     gene            complement(15293..16426)
                     /locus_tag="VGQ51_11280"
     CDS             complement(15293..16426)
                     /locus_tag="VGQ51_11280"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEV9037199.1"
                     /translation="MNRTSFFVMSIFAGMLAACGNHANVPADALPDPAAFKGTTDGQP
                     TALYILKDQNGAAAAVTNYGARLVSLVVPDKNGTPTDVVDGYDNIGNYLHQPETFFGA
                     IVGRYGNRIAKGKFTLDSNTYTLPINNAPNSLHGGTKGFGDVVWTGHQLNDSAVELTY
                     LSKDGEEGYPGNLNVKVTYTLENGNALHINYEATTDKATVVNLTNHAYFNLNGQGSGA
                     IAGHLLQINADRYTPVDSTLIPTGAIDSVAGTPLDFRQPTAIGARVNDTGNQQIRYGK
                     GYDHNFVLDKSKGDLAAIVTGDSSGIVMTVTTDQPGIQFYSGNFMNGTNPLKAGKKDG
                     HRTAFCLETQHFPDSPNEPSFPSTVLKPGEQYHSTTTYAFSVK"
     gene            complement(16443..>17451)
                     /gene="galK"
                     /locus_tag="VGQ51_11285"
     CDS             complement(16443..>17451)
                     /gene="galK"
                     /locus_tag="VGQ51_11285"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV9037200.1"
                     /translation="KAICMALSRRDDDELHIISLDFNQTYTGSIRRISPSGMHWPDYI
                     LGVVEQLQSMGCPTGGFNLVFGGDIPPGAGLSSSAALECATAFALNELFGLSLDRLTM
                     VKLSQAAENEFVGLKCGIMDMFASMFGQKEQVIRLDCQSLAYTYFPFRMDGIRILLLD
                     SRVKHSLASSQYNVRRQQCEAGIRLVQKIHPAVRSLRDVTMEMLQECVAPADDLIWNR
                     CSYVVEENARLQAATEDLQRGDLASFGGRMYGSHDGLSRKYEVSCPELDFLVDQVREE
                     PGVLGARMMGGGFGGCTINLVKEDAIDGLVQRLAPFYRKTMNKEMKVYIARIEDGTST
                     L"
BASE COUNT         3882 a         4891 c         4758 g         3920 t
ORIGIN      
        1 atatttcagt ccttattttc tctggcagca ggccatgaac catgacttca gcggtgatgc
       61 ctactaccgg ttcagcatcg aggtcggctt cctggaatgt ttgctgaagg gcgggtttgt
      121 tctcttcctg ctctatataa cgccaatggt cgcggccatc agaaggggtc ttttttcccg
      181 gcataacaac aaggtagcgt acctgatcgc catttatgtc cttgtcgagc tcctgatcct
      241 gttcgtcgaa aatatccctt cctggcattt tcagttcttt ctgatctttt tcctggcggg
      301 ctactgttgc agacagacgg cccttcaaca atcaccggca aatgaaaatt tccgttataa
      361 cgccctcctt caaccagggt cgctttattg aggatgcgat ccgaagcgtg ctgatccagg
      421 attacccgga tttcgagcac atcgtcatcg acaattgttc ctgggacaac accctcgacg
      481 tgttgaagaa atatccgcat atccggtgga tatcggagcc tgaccatggt cagagccatg
      541 cgctcaataa aggattcgaa atggccaccg gggatatcct ggcctggttg aactgcgatg
      601 atttttattt gcgcgacgct ttccatacgg cggcccgggt cctcggcgac ccgaacatcg
      661 acgggatcta ttccgatctg caattctgcg atctaaactg gcggatcacc aagcactacc
      721 agtcgcaccg tccggtgaaa ttcctgtcgt tgttccacaa cttcatttca tccgagtgct
      781 tctttttcaa acggaagatc atcgacaacc agatccatgt ccgtgaggac ctgcattatt
      841 gcatggacca ggatttcgct gccaatattt tacatcacaa ttaccggctt cactatatga
      901 aggcctgttt cgcggtattc cgctggcatg gcgccaataa atccgtcgat acgccctgga
      961 gacgcgacca gcgtacgcgc gaagggatcc gcatcttcaa ccgtcacaat ggctatatcc
     1021 gcctggatga agacaatccg aatcaccgga aactgtattc atggggccgt accctcctga
     1081 aaccttatcg tctttttctg aaagcaacta acaacatcga tgacaagata catcaccaca
     1141 agctgggacg atggccatcc ttcggacgcg cgcctcgccg ctctgctggc caaatatgac
     1201 ctgaaggcga ccttctatat cccgaaggcc aatccggggc atcgtgtcat gaaggaagag
     1261 gacatccttg ggttgtcccg gcatttcgag atcggaggtc ataccctacg gcatctctcg
     1321 ctgaaagagc tgtcgcacta tgcggcatgg gaagagatcc agggaagcta tgaatggctg
     1381 cgatcgctgc tggggaagtc cccgatgagc ttctgccctc ccttcggaga atacaccgcc
     1441 cggcatctct ccctgatcta tgaaacaggc ttcaaggtgg tgcgttccac ggagctcctt
     1501 tccacacgcc catcgtcagg cgcgatacca actactttac aggtgtatga ccatagccgg
     1561 ttcacctatt tcaaacacct gctcaaacgg gggcggttgc agaaccttgg tctctggtgc
     1621 ggatcgggtt tttcgtcgga cgtcttccgt ttgctggatc actacctgga acacctgatg
     1681 gcccatggcg gatgtcttca tctctgggga cattcctggg agatcgatca ctaccgtcaa
     1741 tggaaaagac tggaaaagat cttttccgtc atcagccacc tctccgattt ctcctatgtc
     1801 gagaacggac agttgtcaaa aacaaatagt cccgtcatat gaaagtcctc tgtatccaca
     1861 cttattacat gcaacgcggc ggtgaagacg ccgtcttcga aaatgagctg cgactattat
     1921 cggatgcggg ttgcgagacg ctgccgctgc ttttcaacaa ccggcgtttt gccgcgctga
     1981 aattcctgtt tttctttttc aatccgcttt ccttctggaa gacctgccgg gccatccgcc
     2041 ggtggagacc ggacatcgta cacgtacaca actggttttt ctccgccagc cctgcggtat
     2101 tcctcgcggc ccgctgcatg ggggttcccg tcgtccatac ggtgcataat ttcaggatac
     2161 tctgtccatc gagcttcctc ttcgtgcgca atgaattgtt caccgactgt atgacgcgca
     2221 tcttcccgct gcgggcgatt acccggaaga tatatcgcgg atcggctttg tccacggcct
     2281 ggctgctcat cggcacgcgg ctccactatt tccttcgcac ctggcaaaag atcgatctgc
     2341 tgatctgtct cacagatccg gcccggcaga tgatttgtca gagtctgctg aagatagacc
     2401 cgcaccgcat cgcgatcaaa cccaatttct ttaaggtgga agagttgccg gtcccgcagg
     2461 gcatggtacc cgcaagacgc tgcggtttcc tttttgtcgg aagactttcg cccgaaaagg
     2521 ggatccagct actgctggac agtttccgga acagcccata cacgctccgg atcgtcgggg
     2581 acggcccgtt gcgcgccgaa gtggaggctt gcgcggctga gtatagcaat atccaatatc
     2641 tgggtctgca gacaaaacaa cagatcatcg gacaccttcg atctgcggag gcactggtct
     2701 tcactaccat cggcttcgag cagtttggtc tcgtcatcat cgaagccttt tcgtgcggga
     2761 ctcccgtcat cggtccggat accgggtccc cggccgagct gatcatcgaa ggatacaacg
     2821 gtctgcattt caagacgggc tcggtccccg atctgcggcg caagatccgg tactggcact
     2881 cgctgcctaa ggaagtccgc gaggtctact ctgaaaattc tgcggatacc tatggccgtt
     2941 actatacccc cgagatcaat ctacgcttat tatctaacat atataaaaag gtgttacatg
     3001 aaaacaatgc aaatcttcaa cttccccatc agcgtcggga agtaccagga ctacattgaa
     3061 tacataatgg acagcgcctc caccgggagg agccagtaca tttatgtagc gaacgtgcac
     3121 atggtcgtgg aagccttttg gaacaagtcg ttctccgaaa aggtgcgaaa ggccgggctc
     3181 gtgacgccag atggcaaacc gctgacctgg gcgttgcggc tgttgcatgg cataaaacag
     3241 gacagggttg ccggaatgga cgttttgccg gccctgctca aacagtccga gaaatctcaa
     3301 actcccgtct atatttacgg cgggacggag acccttcttt cccggacgag aaattacctc
     3361 gaccgcaatt atcccaaact ttgcatcaag ggactgtaca gcccgccgtt ccgcgacctt
     3421 acgccggagg aggaggccag gaccatcgaa aggatcaaca gcagcggtgc gaaactggta
     3481 ttcgtcgtac tcggctgtcc caaacaggag cagtggatgg cgtcgatggc cggcaggatc
     3541 aatgcggtca tgatcggtat cggaggtgcg ctgcctgtgc tcatcggcat gcagaaacga
     3601 gctccccggt ggatgcagaa catgggattg gaatggctgt tccggctatg gctggagccg
     3661 cgtcgtctgt tcaaacggta ttttgttacc aattccgtct ttctttacct gctgagccgc
     3721 gaactcgtga aaaaaacctt tgtcaggaaa ttcgaaccgc atgaaatgaa ctaatacacg
     3781 atgaagaccc tgatctgcga tctcaatgtt tatgataacg ggcaccacat cgccttcgtt
     3841 aattctatcc tgagccatac ggcccggcgg gaagatctca ttttcctgtt caacggtatg
     3901 gcggctacgc tttgtaaagg gatggaaggc aacgaccgga tattcttcgt gcccgaagac
     3961 aatctgcacc ccgatgagct gggcagtctc cgggataaaa tgcgcgaata ccggttcatc
     4021 cggaacttcg cattggagca tggtgtcgaa cgcgtcattt tcctggaaat agactggtac
     4081 caggtagcga tcggtttgag cagtcgcccg ccgttccgca ttaccggcat ttattttcgc
     4141 ccctttcatt tgataccgac acgtggtgac agtctccggg attcggtgaa acgcctgctc
     4201 tatcacgcga aaaaacgctt cctgttccat atcctgaagt tcaacaggaa ggtagaaccg
     4261 ctgttccttc tgaacgaccg gatggccggc gttcgttatc cgtcgtggtt ccgatatctc
     4321 cccgaccctg tattcggcgg tggggccggc cccggcggag gcgggtccgt aactatccgg
     4381 aggcagttcg acatcccttc ctccagccat atcatcctgg ccttcggggc catgggggcg
     4441 aggaagaata tcggcaatat cgtcgcggcc taccagcagg cgaactttga aggagacacc
     4501 gtcctgctca tcgcaggcaa ggtcagagcc gactatcggg gggccttcaa ccgggccatc
     4561 gacagcttcg gatgcaataa cgacggtcgc aagcggctta tcgtattcga caccttcgtc
     4621 gacgaagaac atatcgatct ctatttcagc gaggccggca ccatcctgtt atgctacagc
     4681 aaattctatg ggtccagcgg gctgatgggc aaggccgccc agcaccggaa gacctgcatc
     4741 gtccctgact gcgggctact gtacgacctg aacagggaat acaaactggg ctattcggtc
     4801 gacccggaaa atgtagacag tatcgcgaac gcgctttccc tcgcccagcg ggcgccggtg
     4861 gaggaagcgg gtttccagcg ttttgtccgc gatcatcatg aaagcgcctt tcttcggaca
     4921 ttgcttaccg tttaaaatgt ttatgctatg aacaacaggt tcgtcaacca tctccaggtc
     4981 accctgatcg tgctggacct ggccgtcatc aatttcatct tcttcttctc ccaatttctt
     5041 ttccggcgga gcctggccat tacggccgtc ctggaatacg actatttcct gtatttcatg
     5101 aatatctgct ggatactcac gacctcgctc acgggggtct acaacagcaa gacgatcgtc
     5161 aacttcgaga ctttttcgag gaggagcatg aacgcctttc tctattttac cgggttcgtc
     5221 acgctgtatc tgtatttttt ccaccagatc atcatcagca ggatgttcat agtcgcgcaa
     5281 ctggtcggga tagccgttct catgctggcc aaccgtttta tcttcctggg catctatcag
     5341 tatttcagga aaaaggacca tttgctgcgg aagatcgtcg tcatcggcta taacaacgtc
     5401 gcgaagaaac tggtgcaaca cctcgagaac caacccttca actcgcgggt gatcggattc
     5461 tgcgaagaca cggagaatgt acgcgaggtg acgcattatc ccatactgag caatatcagg
     5521 ggcgtactcg aagaatgcga gcggcagggc gttaccgaga tctattcgac catcgccccg
     5581 gaacagaatg cttatctgta tgagctgata cggggggccg aacggcagtg tatccggttt
     5641 cgcctggtgc ccgatctggg agtgttcatg atgaaaaaac aggtgtatat cgattatctc
     5701 gaggacatgc cggtgttatc acttcgtcgc gagcccctgg aagacctcgg caaccagatc
     5761 cgtcgccgca tcttcgatat cattgtcagc tcgctggtgg tcatctttgt cctctcgtgg
     5821 atggtgccgc tggtcggact ggccatcctg ctggattccc gggggcctat ctttttcaaa
     5881 caggcccgct cggggaagaa caaccgctcg ttcccctgtc tcaaattccg gagcatggtc
     5941 aaaaacaata cgaccgaaca ccggcaggcc acccgaaacg acagccgggt cacccggata
     6001 ggccgcttcc tacgccggac gagcatcgac gaattcccgc aattcctcaa cgtactgaaa
     6061 ggcgatatga gcatcgtagg gccccgtccg catatgctca aacataccag cgattattcg
     6121 cagcagctgg atcatttcat gatccggcag ttcgtcaaac ccggtatcac cggctgggcc
     6181 caggtgcacg gttaccgcgg cgagacccgc gaccttggac agatggagaa aaggatcgaa
     6241 cacgatatct ggtatctcga gaactggtct ttatggctcg acgtgcgcat cgtctttatg
     6301 accgtattca atctcttccg gcgcgaggcc aatgcatatt gatcccgtct tcaccggcaa
     6361 tttccttcgt tcggccgcct aaaaaggcgg ccgaaccgat tatagggcca tcccaaacga
     6421 gattttccta attttacgcc gctatgagtg aaaaaagaca aaaaaaggcg gtgcaggccg
     6481 ggccgggtgg gaaacccatg ggacccggcg ggaaacccgc cgggccggga ttgttctcga
     6541 tattgaaacc gtaccggcaa tggatcattc tcctcgtcgt actgtccctc gtcagcaatg
     6601 ggatcaattt gctcattccg aacatcagca agaacgcgat cgacgcattc atcgcccacc
     6661 ggctggatgt cgaccgcacg ctactgctct ttctgtcggc gatcgtcggc gtttttgtct
     6721 ttacctatgc ctcgtctttt gttcagacct acacctccga gcgggtcgcc cgtgatctcc
     6781 ggacccggct cgcagaccgc atttcgcggc agaattacag ctttctccaa aagaaaaata
     6841 ccggtcagct cctgaccaat ctcacctcgg acgtcgattc catcaaagga ttcgtcgccc
     6901 aggccatcgt ttcgatcttc tcctccatct ttatcatcat cggggcaagc atcttcctgc
     6961 tgacgatccg gtggcagctg gccctgtgcg tcatcgcgat catcccgatc atcgggttca
     7021 cattttttat cgtcctgaaa aaagtgaggg tcttgttcaa ggccagccgg gaggtcatcg
     7081 acacgctgaa taaggtgatc aatgaaagca ttatcggcgc cgcacttatc cgggtgatcc
     7141 attcccaaca cctggaatac accaaattcc tggaagccaa cgccaaagcg ctcggactcg
     7201 ggctcaggat cctcaaatta ttcgccaccc tgatcccgat catcatcttc actgccaatc
     7261 tcgccacgct gaccatcctc gcgtttggcg ggcattatgt catcgcaggc cggatgacga
     7321 tcggtcaatt taccgctttc aatatgtatc tcaccatgct gatcttcccg atactggtca
     7381 tcgggttcat gagcaatgtc atcgcgcagg cgcaggcgtc ttatggccgt atcgctggcg
     7441 tgttgtatac cccggatccc gaagagggcg gaacacgtgt cagcgatctg aagggggata
     7501 tcgattttaa ggatgtatcg gtcagctatg gcgaaaagat ggtattgaag gatatatcct
     7561 tccatatcca tggcggatcg aagacggccg tcatcgggcc gacggctgcg ggcaagacac
     7621 aactgttgta catgcttacc agcctcagcc atcccacccg ggggaccatc gagttcgacg
     7681 gatatccgct ggacggatac cggaaggatg atttccaccg gcaggtcggg ttcgtattcc
     7741 aggacagcat cctgttcaac ctcagcatcc gcgagaatat cgctttcagc gataacgtca
     7801 gcgatgagtc gcttaaaaag gcgatcgcaa ccgccgaact taccgccttt atcgacgagc
     7861 ttcccgaagg gatcgatacc gttgtctccg aacggggtac caccctatcg ggtggacaga
     7921 aacaacggat catgctggcg cgcgcgctgg cgctcgatcc gcgtgtcctc ctgctggacg
     7981 actttaccgc gcgggtagat aaccagacgg aaaagaagat actggaaaat gtccgcgcca
     8041 attacccggg tatcacattg ctctccgtga cgcagaagat cgcttccatc gaagatttcg
     8101 aacagatcat cctgctcatg gaaggagaaa tgatcgcgat gggaacacat gcacaattga
     8161 tgcagacctg tcctgaatat gtacagatct atcaatcgca aaaaagcacc agccactatg
     8221 aacagccgga attacaatct taacgaactg accgcgggac gctcaaaaag caagatatgg
     8281 cccgcgctga cgaaattggt cggcctgcta cccgaacaac gcggcaagct gatcggcgcg
     8341 ttgctgatca tggtcgtcta ctccatactg tccatgctcc cgcccgccct catcgggtac
     8401 accattaacc accttatcga aggtaagatc aacacggtat ccacggggat accggaccaa
     8461 tcgacatgga tgacacacct gtttcacggt tccggctatt cgcttatcgt gacggtatgt
     8521 tgttgtttga tcggtattta cctgttgaac cttggcgcgg tctactggcg gacgatcctg
     8581 atgggcggat tcgggcaaca tctcctgtat acgctgcgga atgcggtctt caacaaactg
     8641 caggaattgc ccgtcgcctt ttttgcgcag aacaaggctg gagatctcat ctcgcggatc
     8701 aacaacgata ccgataaggt caaccaattc ttcagccagt cgcttatgca atttatttcc
     8761 agcatcttca tcatcgcggg cgccggtgtg gcgctggttt atttcaactg gcgtcttggg
     8821 ttgctgacgc tttcgcctgc ggccctgatg tggctattca acaaggtgat gtcgaaatgg
     8881 gtaaagaaaa agaatgccgc cagcgccaaa gcgaccggcg gacttagcgc ggagatacag
     8941 gaatcgctca acaatttcaa ggtagtgctt gctttcaacc gcagggatta cttccgcaaa
     9001 cgtttcggac aggccaatac ggcgaattat tcttccgcgg tgggcgcagg catcgccaac
     9061 agtgtcttta acccggtgta cagcctcttg tccggtctcg gacagatggt ggtccttttc
     9121 tatggcattt atctgatcag gaccggctat ttcacccccg ggttcctggt cgcctatttc
     9181 atgtacacca gcagctttta tgaccccttg cgtcagctgg cggccctctg ggccggattc
     9241 caggtggcca tcgcgagctg ggaccgcatt tcgcatatcc tctcgctgga taccgatctg
     9301 gtgaccatcc atgaatcgga tatcccggca ataccccccg gtgggctttc ggttgtccgg
     9361 acagggaatg gcgcgtcggc aggccagccg ccactgatcg agttccgcga agtatccttc
     9421 gcctatccgg gaggaaaaga tgtgcttcgg cataacagtt tccggatgga aaaaggaaaa
     9481 acgtatgcgc tggtcggccc gacgggcggc ggcaagacta ctacggcatc gctgctggcg
     9541 cgtctgtatg atccgacggc aggggcggtc attctccatg gtcgcgacat ccggacctat
     9601 actcccgccg aacgggctga aaagatcggc ttcattttac aggatcccat ccttttctcg
     9661 ggaacggtcc gggaaaatat cctttatggg aatgagaaat acaaagacgg cccgaacgag
     9721 caattgacag gggtcctgca ttcgtcaggg ctcgagacct tgttgacacg cttcgatgac
     9781 ggcctggaca ccgcggtcac cctcggggga gaagggatca gcctcggtca aaaacagctg
     9841 atcgccttta tcagggccgt gctccgcgag cccgagctgc tgatcctcga cgaggcgacg
     9901 gcgaatatcg ataccgtcac cgaaaaaatg ctggaagata tcctgcgccg tctgcccccc
     9961 accactaccc gcgtgatcat cgcgcatcgg ctcaatacca tcgcaggcgc cgacgagatc
    10021 tttttcgtca atgccggcga gatcacccgg gcgggaagtc tcgatcaggc tgtcgatatg
    10081 ctgatggagg gcaaaagggt gagctgaggc tccggcatcc cgtgagctga ggcttcggct
    10141 tcccgttagc tgaggctccg gcatcccgtg gactccacct ccagactccc gtcagaattg
    10201 ggacctagtt tgggacataa aaacaatgct tatgcaacgc ttgctgacat gttcttttgc
    10261 ttcactggca ctctgcctcg cggttcacac cgcgaatgcg cagagcggtt acgccgttac
    10321 gggtacgttc aggatcgcaa gtccgggcgg ctgggattat atttcggtgg gaccggataa
    10381 gaaggtctat gtctcacacg ggacgcaggt caatatcctc gaccagaaga ccggcgactc
    10441 ggtcggcgtc atccccaata ccaccggcgt ccatggcatt gctttcgacg aacatcacgg
    10501 gaaaggatat accagtaatg gccggctgaa caatgtgacg gttttcgata tgaagactga
    10561 cgccatagaa ggtcagattg cgacagggca gaatccggac gcgatcatgt acgagccgtt
    10621 cacgaaaacc attataacct gcaacggcag aagccataat ctttcgatca tcgattgcgc
    10681 ttcgggcagg gtgacggatt cgatcgcgct cgacggcaaa cccgagaccg ccgtatccga
    10741 cggcgaaggt atggtctatg tcaacatcga ggacaagaac gagatctcgg aggtggatat
    10801 gcgtaaaaaa acggtcgtcg ccagctggcc gcttggcgcc gaaagcccca ccggactggc
    10861 catcgatctg aaaaccaaac ggctttttgc gggttgtgaa aaacagcttg tcgtcatgga
    10921 tgcaaccaat ggttccgtcg tcgccaaact gcccatcggc aatggttgtg acggcgtggg
    10981 tttcgacgaa gggctgaaac tggtattcgc ctcctgcggc gaaggcgtgg tcaccgtgat
    11041 cagggaatcg tccgcttctt ctttttccgt tatcgaaaac gtccctacca agcgcagcgc
    11101 ccgcacctgt gccgtagaca gcaggacgca ttccgtctat ctgcccgcgg ccgacaccga
    11161 gccggcgcaa gcaggtcagc gaccgcgcat gataccgggc accttccagg tattggtggt
    11221 gagcaggaaa tagggtaact tggacaggtg aaatacggta gtttgcaaaa ttttatcaac
    11281 ggaggcttcg ccggtgctgg atccggtaga agcctctctg tattgagccc tatcgatggc
    11341 cgcgcgcttg cggagatgcc gtgttcggac cgtaacgatc tcgatgatgc cgtcgaggcc
    11401 gcccgggcgg ctttccctgc ctggagccgg atgcccgtga aagagcgtgt gcaggtgttc
    11461 ttccggtaca aacggttatt ggaacgcgat atggatactt tggccgcact ggtcagcgaa
    11521 gagaacggaa agacgatgag cgaggccatc gccgagattg aaaaatgtat cgagcttacg
    11581 gaatttgcca cctcgcttcc acaactgata ggcggcgaga tactcgaggt cagcaaagga
    11641 gtggaatgtc gtctcgatca tgtgccgctg ggcgtagtgg cttccatcgt tccgttcaat
    11701 ttccccgcaa tggtaccgca ttggaccatc cccaacgcac tggccctcgg caattgcatg
    11761 atcgtgaagc catccgaaaa agtgccgcta tcctgcgccc gtactgcctt gctgctgcgc
    11821 gaggcgggtc tgccggacgg cgttttcaat attgtcaacg gcgacagcgc catcgtggag
    11881 gctatctgcg accaccccgg tatcgacgca gtctccttcg tcggctccac caaagtggcg
    11941 aagatcgttt accggcgggc cacccagcat ctcaaacgct gtcttgcact cggcggagcc
    12001 aagaaccacc ttctggtatt gcccgatgcc caaccctcca tgacggccgc gaatgtagtg
    12061 gcgtcgatga gcgggtgcgc gggtcagcgt tgtatggcgg cttccgctat ggtcgccgtc
    12121 ggaccggtag atgacattat tggacagata tgtgagggcg cccgcaagat catcccggga
    12181 caaaatctgg gtgcggtgat cgacaggact gcaaaagaaa ggatcgaacg ctatatcacc
    12241 gaagcggaac ggcagggcgc caggatattg gtcgacggcc gcggttatac cgtaccgggg
    12301 aaggaagggg gcagctatgt ggggccaacc gtcatcgatc atgtccggcc cgacatggcg
    12361 atcgctagcg aagagatatt cggaccggtg atctgtataa tgcgtgtcga taccctcgat
    12421 gaagccctcg ccatcgaaaa cgccagcccc tatggcaatg cagcatcggt attcacgcag
    12481 aacggtagct acgcacgata tgttatcgag cgggccagcg ccggaatggt cggtgtcaat
    12541 ataggagtac ccgtgccccg ggaacctttt tcgttcggcg gctggaacga tagtaaattc
    12601 ggcgtctgcg acatcacagg caagagctcg atcgggttct ggacgaagct gaggaaatcg
    12661 accgttaaat ggaatccgga agccgggatc aactggatga cctgaacagc aataaaactt
    12721 ttccatatgc ccgcatccaa cacattatcc gaaacgcagg agatcgtaca ggacaacctg
    12781 gattacacgc ttttctcctg gagcaaacaa cacggtatca accccatcgc cgtacagcgg
    12841 gcggaagggg tctatctgta cgactacgac ggcaaacgct atctcgactt ttcttccggg
    12901 ctgatgaacg tcaacatcgg gcatgggaat caacgggtga ccgatgccgt ggtgcggcag
    12961 atgcaggagg tcagctatgt cacaccgtcc tgtgtcacgc gtgcccgcgg agaattaggt
    13021 aagaagctcg cggagatcac acccgggcgt ctgacgaaga ccttgttcac ggtttgcgga
    13081 gcgaccgcca tcgagaatgc cctcaaggtg gcgcggcttt ataccgggaa acataaggtc
    13141 atcgcgcgtt atcgcgccta tcatggaagt tcgtatggcg ccatgaccgc gggcggcgat
    13201 ccccgaaagc ttcagacgga cgcccagcag gcaccgggca tgatccatgt cgaagacccc
    13261 tattgttacc gctgtccctg gaaccaggaa atgtcttcgt gcaatcgcga atgcgtgtcg
    13321 catattcaaa ggatcattga attcgaaggc cctgagaaca tcgccgcaat cctgatggaa
    13381 ggagaaagcg gctcatcggg ctgtatcaaa tatccgccgg attatttaaa gaaggttcgc
    13441 gccttgtgcg accgctatgg tatcctgctg atcgccgacg aagtgatgag cgggttcggg
    13501 aggaccggga aatggtttgc ctgcgacgtg catggcgtgg ttcccgatat gatcgccacc
    13561 gccaagggga tcacggcggg ttatctgccg atgggcgcgc tgatcgtgtc cgatgcaatc
    13621 gcccgatatt tcgacgaccg tgtgctttgg acggggctca cctattccgc gcatccggtg
    13681 gcctgtgcgg cggctgtcga ggtgctgaag atatatgagg acgaaaacct catagaaaat
    13741 gccgcccgga tgggtcgtta cctggacgaa caggcggagc ggctacgcgc ggaacatccc
    13801 tgtatcgggg acttccgcaa taccggattg ctcggtgtct tcgagatcgt aaagaaccgg
    13861 tcgacgaagg aacccatggc ccctttcaat gcaaagcccg atgagatggc ggtcatgaac
    13921 aaggtggccg cccggctgaa agaggttggt atgtacacct ttgtgcgctg gaattatttg
    13981 ttcgtcgcgc cgcctttgtg tatcacgcgc gatcagatcg acgagggcct ggccatgatc
    14041 ggcgacgccc tgaccatcgc cgatgcgtac gtaaccgaat gagatgcgta tatatttcct
    14101 gttccttatt tttgttgcca tgacagcgac tgcacagcgc gcacgtgact atggcgtccc
    14161 atttgacggg caccccggcg ctttcaatgc tattaccgac gtaccgggtg tcacggtcgg
    14221 ccagaccacc atcatcagcg gcgagggacc gctcgtaaaa ggcaaaggtc ccgtgcgtac
    14281 gggtgtaacg gctatacttc cccgtggacc gaagttcgga ccggtgtatg ccaactggta
    14341 cacgctcaat ggcaacggcg acatgaccgg tacgcactgg atcacggagt ccggttttct
    14401 cgaaacgccg gtcctcatca caaatacggg cagcgtaggc acggtcaggg atgctgcctg
    14461 gcagtggatg gatcagcacc attattatgc gcctttctat aaagattact ggtatgctta
    14521 tcccgtggta gccgaaacct atgatggcat ctttaacgac atcaatggtc agcacgtcac
    14581 aaaggcggat gtctttcatg ccctcgatag cgcccgttcc ggactggtcg aagaaggagg
    14641 cgtcggaggt ggcaccggca tgatgtgcta taattttaaa ggagggatcg ggacggcctc
    14701 gcgggtattg gatgcccgcg acggcggcta tacggtcggt gtgctggtac aatgcaacca
    14761 tggcagccgg ggacagctga ccattgcagg agtccctgtc ggccgccggc tgcaggatac
    14821 gctgccctat atcatcgaag ggcaatcttt cggggtgacg gatgcccttc cccgggatga
    14881 gaccggctcc atcatcgtca tacttgcgac tgacgcacct ttgctgcccg gtcagctcaa
    14941 acggctggct caacgtatac ccctcggcgt tagccgtacc gggggcatcg gcggtaatgg
    15001 ttcgggcgat atcttcctgg cgttttcgac cgccaacccg caggcattca gcaatacgga
    15061 aaacagggag gtcaccacgc tgcccaacga ccggatcaat ccccttttcg aggccacggt
    15121 cgaggctacg gaagaagcca tagtcaacgc gctgttcgcc ggtaagacgc tggtcggtat
    15181 caacgggaat accgtacccg gtctgcccaa agaagcggtg atccgcatcc tgcgggaata
    15241 caaccggctt gcgccggggc actgaaagca cggcgggacg ctgagcgagc gcctacttca
    15301 cgctaaacgc atacgtggtc gtggaatgat attgctcccc gggcttcagc accgtcgacg
    15361 gaaaggacgg ttcgttcggg ctgtcgggga agtgctgcgt ctcaagacag aaggcggtac
    15421 gatgaccatc ctttttccct gccttgagcg ggtttgtgcc attcatgaaa ttgccggaat
    15481 agaactggat gccgggctgg tcggtggtaa ccgtcatcac gatgccgctg ctgtcgcccg
    15541 ttacaatggc agccagatcg cctttgctct tgtcgagcac aaaattgtga tcgtaaccct
    15601 tgccatacct gatctgctgg ttaccggtat cgttgacccg cgcaccgatg gccgtcggct
    15661 gacggaaatc taatggcgtg cctgcgaccg aatcgatagc ccccgtgggg atcagcgtcg
    15721 aatccaccgg cgtgtaccga tcggcgttta tctggagcaa atggccggcg atggcgccgc
    15781 tgccctggcc gttgagattg aaataggcgt ggttggtcag gttgaccacg gtagccttat
    15841 ccgtcgttgc ttcataattg atgtgcagcg cattcccgtt ctccagtgta taggtcacct
    15901 tgacattcag gtttccggga tatccttctt ccccatcttt ggagaggtag gtcagttcga
    15961 cggcgctgtc gttcaactgg tggcccgtcc atacaacatc gccgaaacct ttcgtccccc
    16021 cgtgcaatga gttcggcgca ttattgatcg gcagcgtgta agtattgcta tcgagcgtga
    16081 acttgccttt cgcgatacga ttcccataac gccctacgat cgccccgaaa aaggtttccg
    16141 gctgatgcag gtaattgccg atgttgtcat aaccgtcgac cacatcggtc ggtgtgccat
    16201 tcttgtccgg gactaccagg ctgaccagcc gggcgccata gttggtgacg gccgcggcgg
    16261 ccccattctg gtctttcagg atataaaggg cagttggctg cccgtcggta gtacctttga
    16321 aagcggcggg atccgggagt gcgtctgcag ggacattcgc atgattgccg caggcggcta
    16381 acatgcccgc gaagatgctc attacaaaga aggaggttct gttcatatag caatgattgt
    16441 atttatagtg tcgaagtgcc gtcttctatc cgcgcgatat acaccttcat ctccttattc
    16501 atcgtcttcc ggtagaaagg cgcaagccgc tgaacgagcc cgtcgatcgc gtcttcctta
    16561 acgagattga tcgtgcagcc gccgaaaccg ccgcccatca tgcgcgcacc cagcacaccg
    16621 ggttcttcgc gtacctggtc gacgaggaaa tccagttcgg gacagctgac ctcatatttc
    16681 cggctgagcc cgtcgtgcga accatacatc ctgccaccga aggaagcgag gtcgccgcgt
    16741 tgcagatctt cggtagcggc ctgcagacgt gcattctctt ccacgacata ggaacagcgg
    16801 ttccagatca gatcgtcggc cggagccacg cactcctgca acatctccat ggtcacatcc
    16861 ctgaggctgc gaacggcggg atgaattttt tgcaccagcc tgatccccgc ctcgcattgt
    16921 tgccggcgga cattgtattg cgaagatgcc agcgaatgtt tgacacggct atcgagcagc
    16981 aatatgcgaa tgccatccat ccggaaagga aaataggtat aggccagcga ctggcaatcg
    17041 agccggatga cctgctcttt ctgaccgaac atgctcgcga acatgtccat gatcccgcat
    17101 ttcagaccga cgaattcatt ttccgcggct tgtgacagtt tgaccatcgt cagccggtcg
    17161 agcgacaaac cgaagagctc attcagcgca aaggcggtcg cgcattccag cgccgcagaa
    17221 gatgaaagac cggctccggg agggatgtct cccccgaaga ccagattgaa ccctccggta
    17281 ggacagccca tgctctgaag ctgttcaacc acgccgagta tatagtcggg ccaatgcatc
    17341 cccgaaggag atatgcgtct gatggagcca gtataggtct ggttgaaatc gagcgatatg
    17401 atgtgcagct cgtcatcgtc gcggcggctt agcgccatac agatggcctt g
//
LOCUS       DASYSV010000367         6528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_71902, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000367 DASYSV010000000
VERSION     DASYSV010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71902"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VGQ51_11290"
     CDS             <1..1106
                     /locus_tag="VGQ51_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEV9037201.1"
                     /translation="EYHHLVRRLKDGRVINVLYKNKNNEPRQDTLWNIVADAAVVVLP
                     YIEKTFGPYPWKQYSFVHGGDGGMEYPMSTLINGPSLGTAFHEWMHSWYQGMMGTNES
                     MYPWMDEGFTTYAEQRVSAWYHREEARLHPEDQGLQDRVKQDSAIGLPLFHSRSYSSY
                     FALAKSGLQEPLTTHSDHYNTNFAYEIAAYSMGDMFLEQLGYIVSDSVRDRILLEYYR
                     EWRFKHPNANDFVRVAEKVSRVKLDWYKEYWINTTKTIDYGIDSLWEQDGKTKIRLKM
                     VGNMPMPLDVTVTYRDGSKEMAYIPQYLMFGEKPREDWTMPWTVYEAWKWTDPTYTFT
                     VNHRLTDIKAVEIDASQRMADVDRKNNKLELNW"
     gene            complement(1364..2032)
                     /locus_tag="VGQ51_11295"
     CDS             complement(1364..2032)
                     /locus_tag="VGQ51_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9037202.1"
                     /translation="MTISVCIVDDNKDIRSALEQIILMADGYTLAGSYSDAEEALQKI
                     PGAKPNIVLMDINLGEGDNGIDCVRQLKPQYPDILFMMCTVYEEDEKIFEALKAGANG
                     YILKKTAPGKLLDALRELYEGGAPMSSQIARKVVNAFQVRTPGDDPTSDAGTTTASPK
                     TISILSNRENEILELLARGMLYKEIAARLYISQETVRKHVYHIYEKLHVNNRVEAINK
                     FYGR"
     gene            complement(2133..2795)
                     /gene="rsmG"
                     /locus_tag="VGQ51_11300"
     CDS             complement(2133..2795)
                     /gene="rsmG"
                     /locus_tag="VGQ51_11300"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV9037203.1"
                     /translation="MEKASPGLEIVLQYFADFSPAQLQQLTALGPLYREWNAMINLIS
                     RKDIDSLYEKHVLHSLAIAAAFSFTPGMQVIDIGTGGGFPGIPLAIFFPETQFHLVDS
                     IGKKIKVVDAVRDAIGLTNVTTRHGRAEEIKDRKFDAAVSRAVAPLKELWNWTRPLLR
                     KSSPSTTSSGEARTSGLICLKGGDLAAEISASGLKPRIMEINELFPLDYFNEKYLLQV
                     NP"
     gene            complement(2765..3895)
                     /locus_tag="VGQ51_11305"
     CDS             complement(2765..3895)
                     /locus_tag="VGQ51_11305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9037204.1"
                     /translation="MAKLFLSFPWLELSFIGFCVITLIQLFYYGWFFKRLAFYSPKTK
                     EHNQQHPVSVIICARDEAARLVTHLPGSLSQRYPSTHEIMVVNHNSQDETRYLLDEFK
                     KTWKGLHIVNLEHEAKGIPGKKYPLSIGIKEAQYEIILLTDADCIPATEFWIQKMQDA
                     YFNGIEVVLGYSPYKKAPGILNKIIRFDTFHTALQYLSYALAGNPYMGVGRNLSYKKD
                     LFFRNKGFSALNHVPGGDDDLFINKVATRTNTAIVVDPDTFTVSEPKKTFPEWFRQKA
                     RHHTTAKYYKPRHKFLLGLYSITHLLFYPLLIVSLFFNWRLTLIAFGSRFIVQGFIFH
                     RTMRRLGEEDLFPWWWALDIWMFFYYIIFASTVWKKPRQAWK"
     gene            3975..5114
                     /gene="tgt"
                     /locus_tag="VGQ51_11310"
     CDS             3975..5114
                     /gene="tgt"
                     /locus_tag="VGQ51_11310"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV9037205.1"
                     /translation="MASLEFRLEGHCPQTAARAGRVTTDHGVLETPVFMPVGTVGSVK
                     ALTQQQLKEDVKAQIILGNTYHLYLRPGLEVLERAGGLHRLNGWDRSILTDSGGYQVF
                     SLAGTRKISEEGVMFQSHIDGSRHLFSPEKVMDIQRSIGGDIVMAFDECTPYPSEYGY
                     VEKSMRLTHRWLDRCWERFHEMPDKYGYTQNLFPIVQGGVYADLRKQSCEYIAAKGAV
                     GNAIGGLSVGEPEQQMYDICQACCEALPEERPRYLMGVGTPWNILECIGLGVDMFDCV
                     MPTRNGRNAMLFTSRGVINIDNKKWEFDYSPIDEGIGSNFSAYYTKAYLRHLVKAKEI
                     LALTIASVHNLTFYLWLVGEARRHILQGDFEGWKREQCEVLRQRL"
     gene            5148..6242
                     /locus_tag="VGQ51_11315"
     CDS             5148..6242
                     /locus_tag="VGQ51_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEV9037206.1"
                     /translation="MFKLIDNYILKKFLGTFLFTMLIVTVIAVVIDTSEKADDFVRSG
                     LTARQLITAYYIGFIPFIMSMIYPLMVFIAVIYFTSKMAGRSEFVAILAGGVKYNRML
                     RPYMVGAIFLSLIFWLASQYWVPKANEIRTDFQLVYVDRNSSYNSDPYRNNNYYLRVD
                     PNTFVGFKYYDTANKTASSFFLQHLKGNKVTYNVRAETVRWDPQKKDWKLTTVVERTI
                     DGVKETLKKMDSLHVNLNVQPKELRRDDYLKDKLTTPELKQFVHMEEVRGAEGLNTFK
                     VELYHRDATPFSVIIMALIGAVMACRKVRGGSGLHLAIGIVLAAIFVVMDKFSVTFST
                     KGNLPPILAAWLPNIIFSGVAGILYAKAPK"
BASE COUNT         1506 a         1323 c         2172 g         1527 t
ORIGIN      
        1 cggagtatca tcatcttgtg aggcgtctca aggatggccg ggtcatcaac gtattgtaca
       61 agaacaagaa taacgaacca cgtcaggaca cgttgtggaa tattgtggcg gatgcagcgg
      121 tagtcgtctt accgtatata gagaagactt tcgggccgta tccgtggaag cagtattcgt
      181 ttgtgcatgg cggagacggc ggcatggaat acccgatgag tacgctgatc aacgggccca
      241 gtcttggaac ggcattccac gaatggatgc acagctggta ccagggcatg atgggcacca
      301 atgaatcgat gtatccctgg atggatgaag ggtttaccac gtatgcggag cagcgggtga
      361 gcgcctggta tcatcgggag gaggcgcggt tgcatccgga ggaccagggt ctgcaggacc
      421 gcgtgaagca ggatagcgcc atagggttac cgttgttcca ttcccgatcg tacagctcgt
      481 actttgccct ggcgaagagc ggattgcagg agccattgac tacgcattcg gatcattaca
      541 acacgaattt tgcatatgag atcgccgctt attccatggg agatatgttc ctcgaacagc
      601 tgggctatat cgtgagtgat tcggtaaggg acaggatcct gctggagtat tatagagaat
      661 ggcgtttcaa gcatcccaat gcgaacgatt ttgtacgggt ggcggagaag gtgagccggg
      721 tgaagctgga ttggtataag gagtattgga tcaatacgac gaagacgatc gattatggca
      781 ttgacagttt gtgggagcag gatggcaaga cgaagatccg gttaaagatg gtgggcaata
      841 tgccgatgcc gctggatgtg acggtgacgt atcgcgatgg ttcgaaggag atggcgtata
      901 tcccgcagta tctgatgttc ggggagaagc cgagggagga ttggacgatg ccgtggacgg
      961 tatatgaggc gtggaagtgg acggacccga cgtatacgtt tacggtgaat catcggttga
     1021 ccgatatcaa agcggtggag atcgatgcgt cgcagcggat ggcggatgtg gacaggaaga
     1081 ataataagct ggagttgaat tggtaggggc cgcagggggc atggaggccg ggcttggagg
     1141 atgggattgg gcgttggagg cgggcgaagg aggatgggat tgggcgttgg aggatgggcg
     1201 ttggaggccg ggcttgggcc ggagggagat aaaatagtcc ggtcggtcgg accggacaga
     1261 agacaaggta aaatgactaa tgggtaatcg tatttgattg tgcgagccct aacaaaggta
     1321 gagggctcgc aaaagacaaa aaataccacg aagtggtagt tttttagcgg ccgtaaaatt
     1381 tgttgatggc ttccacgcgg ttgttcacat gcagcttttc gtatatgtga tagacatgtt
     1441 tgcggactgt ttcctggctg atatagagac gggcggcgat ttccttatat agcatgcccc
     1501 gggcgaggag ttcgagtatt tcgttctcgc gattggagag aatgctgatc gtttttgggg
     1561 aagccgtggt ggtgcccgca tcggaggtcg ggtcgtcgcc gggtgtgcgg acctggaagg
     1621 cgttgacgac tttgcgggcg atctggctgc tcatgggggc gccgccttca tacagctcgc
     1681 gaagcgcatc gaggagcttg ccgggggcgg tcttcttcag tatatagcca ttggcgccgg
     1741 ctttgagcgc ttcgaatatc ttttcatctt cttcatagac ggtacacatc ataaacagga
     1801 tatcgggata ttgcggcttc agttggcgta cacagtctat gccgttgtct ccttcgccga
     1861 gattgatatc catgaggacg atgttgggct tggcgccggg aatcttttgc agggcttctt
     1921 ctgcatcgga gtaggaaccg gccagggtat agccgtccgc catgaggatg atctgttcga
     1981 gggcggagcg gatgtccttg ttatcgtcga caatacaaac agatatagtc ataggacaaa
     2041 ggtctggtgg tttgcggaaa aatcaatacc acgttgtggt agtttttttt catatgactg
     2101 gcggcgcctt cgccttatca ggcgtcccgg ggtcagggat ttacctgcag gaggtatttt
     2161 tcgttgaaat agtcgagcgg gaaaagttca ttgatctcca tgatgcgggg ttttaggccg
     2221 ctggcggaga tctcggcggc gagatcgccg cctttcagac aaatgagacc ggaggtccgc
     2281 gcttcgccgg aggaggtggt tgatggggag gatttgcgca ggagggggcg ggtccagttc
     2341 cagagttctt tgagcggtgc gacggcgcgg gagacggctg catcgaattt acggtcttta
     2401 atttcttcgg cgcggccgtg tcgtgtagtg acattggtga gaccgatggc atctctgact
     2461 gcgtcgacga ccttgatctt ttttccgata ctgtctacga gatggaattg ggtttcgggg
     2521 aagaagatgg cgagggggat gccggggaag ccgccgccgg tgccgatgtc gatgacctgc
     2581 attccgggtg tgaaggagaa tgcggcagca atggcgaggg aatgaaggac atgtttttca
     2641 tagaggctgt cgatatcctt gcgggaaatg aggttgatca tggcgttcca ttcccggtac
     2701 agcgggccga gcgcggtgag ctgttgcaat tgcgccggcg agaaatctgc aaaatattgc
     2761 aggactattt ccaggcctgg cgaggctttt tccatacggt agaagcgaag atgatatagt
     2821 aaaagaacat ccagatatcc agtgcccacc accaggggaa gaggtcttct tcgccgaggc
     2881 ggcgcatcgt gcggtgaaag atgaacccct gtacgatgaa ccggctgccg aaggcgataa
     2941 gggtcaggcg ccagttgaag aagaggctca cgatcagcag gggatagaac aggaggtgtg
     3001 tgatggaata caggcccagg agaaatttat gccggggctt ataatattta gcggtggtat
     3061 gatgccgggc tttctggcgg aaccattccg ggaaggtctt tttcggctcg gacacagtga
     3121 atgtgtcggg gtcgacgacg atggcggtgt tggtcctggt ggcgacctta tttatgaaga
     3181 ggtcgtcgtc gccgccgggc acgtggttga gggccgagaa tcctttattg cggaagaaga
     3241 ggtccttttt atagctgagg ttacggccga cgcccatgta gggattaccc gcgagcgcgt
     3301 aggacaggta ttgcagtgcg gtatggaagg tgtcgaagcg gatgatcttg ttgagaatgc
     3361 cgggggcctt tttatagggg ctatagccta gcaccacttc gatgccgttg aagtaggcgt
     3421 cctgcatctt ctggatccag aattcggtgg cggggatgca atcggcgtcg gtgaggagga
     3481 tgatctcgta ctgggcttcc ttgatgccta tgctcagggg atatttcttg ccggggattc
     3541 ctttggcttc gtgttcgaga ttgacgatat ggagaccttt ccaggttttt ttaaactcat
     3601 cgaggaggta gcgggtctcg tcctggctgt tgtggttgac gaccatgatc tcgtgggtgc
     3661 tgggatagcg ttgggagagg gagccgggaa gatgtgtgac gagacgcgcg gcttcatcgc
     3721 gggcgcagat gatgacgctg acgggatgtt gttggttgtg ttctttcgtt tttggagaat
     3781 agaaggcgag tcgtttgaag aaccagccgt aatagaataa ttgtatcagc gtgattacgc
     3841 aaaagccaat aaacgacagt tcgagccagg ggaaactcag gaacaatttt gccataagtg
     3901 gttactaggg gggcgccggc cttggccggc gccgatgggg cgaaaatacg taaattgcgc
     3961 ggcattagac aactatggct agtttggaat ttaggttaga ggggcactgc ccgcagacgg
     4021 cggcccgcgc cggcagggtc actacggatc atggggtact ggagacccct gtttttatgc
     4081 ctgtggggac ggtcgggagc gtaaaggccc ttacacagca gcagttgaag gaggatgtga
     4141 aggctcagat catactgggt aacacttacc atttgtatct gcggcccggg ctggaggtcc
     4201 tggaaagggc ggggggcttg catcgattga acgggtggga caggagtata cttacggata
     4261 gcggtggata ccaggtattc tcgctggcgg gcacccgaaa gatctcggag gaaggcgtaa
     4321 tgttccagtc ccatatcgac ggatcgcggc atttgttcag cccggagaaa gtgatggata
     4381 tacaacgcag catcgggggc gacatcgtca tggcctttga tgaatgtacg ccgtatccca
     4441 gcgaatacgg gtatgtggaa aagtcgatgc ggttgacgca ccggtggctg gaccggtgct
     4501 gggagcgttt tcatgagatg ccggacaaat atgggtatac acaaaatctc ttcccgatag
     4561 tgcagggagg tgtgtatgcc gacctgcgaa aacagtcttg cgagtatatc gcggcaaagg
     4621 gggcagtggg caatgccatc ggcgggttga gtgtggggga gccggagcag cagatgtatg
     4681 atatctgtca ggcatgttgc gaggccttgc cggaagagcg gccgaggtac ctgatgggtg
     4741 tcggaacgcc gtggaatatc ctggagtgta tcggtctggg tgtggatatg ttcgactgtg
     4801 tgatgccgac aaggaatgga aggaatgcga tgttgtttac ttcccggggg gtgatcaata
     4861 tcgacaataa gaaatgggag ttcgattatt cgcccataga tgaggggatc gggtccaact
     4921 tcagtgccta ttacaccaag gcctatctgc ggcacttggt aaaagcgaag gagatactgg
     4981 cattgacaat agccagcgtc cataatttaa cattttatct atggctggtg ggggaggccc
     5041 ggaggcatat cctgcagggt gattttgagg gatggaagag ggagcagtgc gaggtgttgc
     5101 gacaaaggtt gtaaatttgc ggccaccggg cgaaatacac ggaacctatg ttcaaactga
     5161 tcgataatta catactgaag aagtttttgg ggaccttcct gttcacgatg ctgatcgtga
     5221 cggtgatcgc ggtggtaatc gacacgagcg agaaggcgga tgatttcgtc aggtcgggtc
     5281 ttacggcgcg gcagttgatc acggcgtatt atataggctt cataccgttt attatgtcga
     5341 tgatctatcc gctgatggtg tttatcgcgg tgatctattt cacgagcaag atggccggcc
     5401 ggtcggagtt cgtcgccatt ctggcggggg gtgtcaaata caaccggatg ttgcggccgt
     5461 atatggtggg cgccatattc ctgtcgctga tcttctggct ggcgagtcag tattgggtgc
     5521 cgaaggccaa tgagatacgg accgatttcc agctggtcta tgtagaccgg aacagcagct
     5581 acaatagcga tccttaccgc aacaacaact attatctgcg ggtggacccg aatacctttg
     5641 tcgggttcaa atattacgat actgcgaaca agacggcaag cagctttttc ctgcagcacc
     5701 tgaaggggaa caaagtgact tataacgtac gtgcggaaac ggtgcgttgg gaccctcaga
     5761 aaaaggactg gaagctgacg acggtggtag agcggacgat cgacggggtg aaggagaccc
     5821 tgaagaaaat ggacagtctg catgtgaacc tgaatgtgca gccaaaggag ctgcggcgcg
     5881 acgattatct caaggacaag ctgacgacgc cggagctgaa gcagttcgtt catatggagg
     5941 aggtgcgtgg agccgagggg ctcaatactt tcaaagtgga gctttaccac cgggatgcca
     6001 ctccattctc agtgatcatt atggcattga tcggcgccgt tatggcgtgc aggaaggtga
     6061 ggggtggaag tggattgcat ctggcgatag ggatcgtgct ggcggcgatc tttgtggtta
     6121 tggataagtt ctccgtgacc ttttcgacga agggaaattt gccgccgata ctggcggcgt
     6181 ggttgccgaa tatcatcttc agtggggtgg cgggtatttt gtatgcgaaa gcgccgaagt
     6241 agaatctgcg atcaggcgag gcggaggaag gagcgggaag agggcgccga agtagaaggt
     6301 aagaaagaat tcatttgcaa tacaacgttc tgaacagtaa ctttaacgca taaacaaggc
     6361 ataatggcat cgattaaaga aaggttcaaa tccaaggccg atggactggc tgctgaaata
     6421 aaagagttgt tgaaggagca tggtgcgaaa acgatcggag aggtaacgct ttcacagata
     6481 taccagggta tgcggggtat tacgggtttg gtgagcgaga cgtcgctg
//
LOCUS       DASYSV010000368         6892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1224965, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000368 DASYSV010000000
VERSION     DASYSV010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224965"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="VGQ51_11320"
     CDS             complement(<1..1930)
                     /locus_tag="VGQ51_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037207.1"
                     /translation="MQSISAPIQLNEVSDKVANLQAALGALGFSIPQEEISRKTAAQG
                     TAKAVHDFQSQNKIPGNTAVLVDQATADFLNRLLKDRKLLDDAGGAAAAKTYTISGKV
                     LNARSEALEGKSVQAFDLDLRGARIYKTAQTLDEITANKGMRPLGSAVSRTDGTYSIT
                     FTNQQLNPAEPGLPDVIVYAVKDNKIIGRSALSTDTNFTNGNLLQGWDVAVAGIADRG
                     PSEFTTLLQQVQDRLRISSVAMNELLDSTDQIDFLATEIEQPVNNVQLLITANQLAQT
                     FPNDPNAHSLLYGLGREAVPLSFSAIALTAEDRLLAAWHQAFDGNIIGTVDDKTLTTF
                     LGQLHQNAAQQMLTTPPIAEAATLSKTLSITTADKNLQAAFITASRTFTGTAEDFWNK
                     YLPSLPGFTPPVIQSLQLTNQLVDLTGNHLPLVEALQERKIQSPSDLLSLTDADWTAI
                     LKTSGIPAGVPGNTDAEKTNNYKGQLQTLLNAAYPTVKIASMISGDQLPIPDATVKQG
                     LKTFFATAGNFDISSSRIIDFQKELVAAGGQNALPLQQNLARIQRLFSVSPTPDTLVQ
                     LMTAGFHSAVQIANIPQQAFVDINAAKLGGVDLAVSVHQRASFQIMRMEKIVGSLHDI
                     VKSVVPGVIQSQGGAQAAQ"
     gene            complement(1978..6846)
                     /locus_tag="VGQ51_11325"
     CDS             complement(1978..6846)
                     /locus_tag="VGQ51_11325"
                     /inference="COORDINATES: protein motif:HMM:NF023676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/C-terminal domain-containing
                     protein"
                     /protein_id="HEV9037208.1"
                     /translation="MPEKLLPATALDSGSDAETCRQAIRACKGSILRKEIYALDASVL
                     QDKPYSAEQHSYVIKTLQPRGDDKYGVFLCHESEAINYHYDRDPGDPRMAHSFIFGID
                     AFGNVLQQASVVYGRQPLPGNEPEQEQLHIVYTENQFTNVIRTNAEYRAPLPYTTRSY
                     ELSGAPKPVVHYAWEDLKKYCITAAFIDFNITPTTGIQKRLLKFSRTQYRGNDGTTVL
                     PPGIMESKGLFDQSYQACFNQDMLTVFSPKIAPADLQVILQDPARGAFVLSDNYYWAV
                     SGTKNYDSAHFYLSTLFTDPFGNKTSLSYDPNYSLFIAKTTDALGNETSVVKFNYRVL
                     TPYLMQDANDNLTAARFDELGLVVSTFAIGKKGVDAGDEFDDSRAEIKGAVDFPGSEM
                     EYDLFEWYNQTLSVGFDLSNYKPKPNTIKTTVRETHYHADPQHSSGTREAYAYSDGTG
                     HVVLSKTQAEPGPALQINPDGSVTTIDTSPNLRWAGNGRTIINNKGNPVKKYEPYFSV
                     DPSFDDEKEMVQLGVTPVMHYDPPGRVIRTDLPDNTFTRVEFTPWLQASYDNNDTVKD
                     SDWYAALGKPDPSDPEPADAQKRAAWLAARDADTPSLVHLDSLGRTFLTIVDNAGDKL
                     SSRIIMDISGNALETTDPLDRKVMEYAYGMTGMKWKQTSMDAGSRWMMGDVAGKPLLG
                     WDDRDHAFRFTYDALGRKVASFVKTGGGSETIAAQIEYGETVATAKADNVKGKVYKNY
                     DQSGISISRQFDFKGNPLQATKQFAKNYKTAVDWTNPVDMEPEIFENAAEYDALNRPI
                     KLVTPHSVTMPPSEITPGYNEAGMLDTVMVKIRGAATPTPFVTKIDYDAKGQREAIFY
                     GNNTVTRYTYQKETLRLVRLLTSRNAGADILQDLNYTYDPFGNITQIKDNAQPDIFFD
                     GEQVQALNKYVYDAVYRLVSATGRKQAGQTDIRPKSDIPGSFPFGGYPFIDSTSINPN
                     DANAFWNYTEQYTYDKAGNLLQQQHVAKNSSWTRSFTYDGGTNGNNRLTDVTLGADTF
                     HYSYDAHGNMSGLETVLNELWDFSDHFKQADLGGGGTVYYVYDSAGQRCRKVIERLDG
                     TSNERIYLGGIEIYRQKTSAGAIALERETLHVMDAQRRIAMVDTPVVKPAGSNETQLI
                     RYNYENHLGSASLELDDAAKIISYEEYFPYGTTSYSTLDATREVPAKRYRYTGKERDE
                     ESGLNYHGARYYALWLCRWTSADPIGIGDGLNMYTYVSDNPVKLFDPGGTDAYSHLPP
                     QKYGYEEKKFDTQSECNANTWDNCTPADGVWGSSGPYYGVVPRTTPSAQIATPPPPPP
                     PPPPKKKATPPPQKPPEEKKGRELSASELTDLAEALGPHYIYPKEYYWVTGTLQLLGS
                     GLEIAGGIYAAPESLGGSLFLVGNGADNGVAAARTIWEGKPVFTLKYHLSYTIASGLG
                     ADEKLSQAIGQSGELTINLAAAGVGFNLASAPISIEEQPVSLAKGTFTGVIKDRFGFV
                     VTDDAAGNLANTTGKAGRFDYAIHGSKEGFWITDHTGEWIKVSPERVAEFMRVDGYSG
                     GPVRLLSCEAGAGVPGLRLNPAQRLANILNEPVYAPTSKLWVYPDGRLIVGPHFSTPT
                     TSGAWQWFKPTF"
BASE COUNT         1448 a         1793 c         1875 g         1776 t
ORIGIN      
        1 cctgggcggc ctgggcgccg ccctgggatt gaatgactcc cggcacaaca cttttgacga
       61 tatcgtgcag gcttccgacg atcttttcca ttcgcatgat ctgaaaagag gctctttgat
      121 ggacggaaac ggccagatcg acgcctccca atttagctgc gttgatgtcg acaaacgcct
      181 gttgaggtat attggcgatc tggacggcgg aatggaaacc cgctgtcatc aattggacca
      241 gggtatcggg agtggggctt acggaaaaga ggcgctggat acgggccagg ttttgttgga
      301 gcggcaaggc attctgtccg cccgcggcga ccagttcttt ttgaaaatcg atgatccggg
      361 aggacgaaat gtcgaagttg ccggcggtcg cgaaaaaggt cttcagccct tgtttcacgg
      421 ttgcatcggg gattggcaat tgatcccccg agatcatcga ggcgatcttt accgtcgggt
      481 acgcggcatt cagcaacgtt tgcaactgcc ccttatagtt attcgttttt tcggcatcgg
      541 tattgccggg aacaccggcc gggatcccgc tcgtttttaa aatagccgtc caatcggcgt
      601 cggtgaggct aagcaggtcg gacgggcttt ggatctttct ttcctgcaat gcttccacca
      661 gcggtagatg gttgccggta aggtcgacca attgattggt cagttgcaag gattgtatca
      721 ccggaggggt aaagccgggt agggaaggga gatacttgtt ccagaaatct tccgcggtgc
      781 cggtgaaggt gcgggatgcc gttatgaaag ccgcctgaag gtttttgtcg gcagttgtta
      841 tactcaatgt tttcgagagg gtggccgctt cggcgatagg aggggtggta agcatttgtt
      901 gtgcggcgtt ctgatgcaat tggcccagga aggtggtcag cgtcttgtca tccacggtgc
      961 ctatgatatt gccgtcgaaa gcctgatgcc aggcggcaag aagcctgtct tcggcggtca
     1021 gggcgatggc tgaaaaagaa agcgggaccg cctcccggcc cagtccgtat agcagggaat
     1081 gggcgttcgg atcgtttgga aaagtctggg ccagctggtt tgcggtgatc agcaattgta
     1141 cgttgttgac cggctgttcg atctccgtgg caagaaagtc gatctggtcc gtggaatcga
     1201 gcagttcgtt catggccaca ctgctgatgc gtagcctgtc ctgcacctgc tgaaggagtg
     1261 tcgtgaattc gctgggcccg cggtcggcga ttccggcgac ggccacatcc catccctgca
     1321 ataaattacc gttggtaaaa ttggtatcgg tggaaagcgc cgaccggccg atgatcttgt
     1381 tatccttgac ggcatagacg atcacatcgg gtaatccagg ttcggcaggg ttcaactgct
     1441 ggttggtaaa agtgatggaa tacgtgccgt ctgttctgga gaccgccgac cccagcgggc
     1501 gcatcccctt attggcggta atttcatcga gggtttgcgc ggtcttgtag atcctggctc
     1561 cccgcaggtc gagatcgaag gcctgaacgc ttttcccctc cagcgcctca ctccgggcgt
     1621 tgagtacttt tcccgagatg gtgtaggttt ttgccgccgc ggctcctccg gcgtcgtcca
     1681 gcaatttccg gtctttcaac aggcgattga gaaagtcggc cgtggcctgg tcgacgagca
     1741 ctgccgtatt gcccggtatc ttgttctgcg actggaaatc gtgaacggct tttgcggtac
     1801 cctgggccgc cgtttttctg ctgatctcct cctggggaat ggagaaacca agggcgccaa
     1861 gggcagcctg gagattggca actttatcgg acacttcatt gagctgaatg ggggcagaaa
     1921 tggattgcat aaaaatgaat ttcagtgaac tttttattgt tatctggtcc tttatgttta
     1981 gaaggtcggc ttgaaccatt gccatgcgcc gcttgtcgtc ggcgttgaaa agtgcggccc
     2041 cacgatcaat cttccatctg gatagaccca aagtttgctt gtcggcgcat acacaggctc
     2101 gtttaagata ttggccagtc tttgggccgg gttcaatctt aaaccgggga cgccggctcc
     2161 ggcttcgcag gacaaaagcc gtactggacc tccggaatac ccgtcgacgc gcataaattc
     2221 ggctacccgt tcgggtgaaa ctttgatcca ctctcccgta tgatccgtga tccagaagcc
     2281 ctctttactt ccatgtatgg cataatcgaa tctccctgct ttgccggttg tgttagccag
     2341 gttacccgcg gcatcatcgg tgaccacgaa gccgaaacga tctttgatca ctcccgtaaa
     2401 ggttcctttt gccagcgaca ccggttgctc ttctatcgag atcggcgctg aagccagatt
     2461 gaagcctact cctgccgcag caaggttgat agtgagctcg cccgattggc cgatcgcctg
     2521 agacaatttc tcgtctgcgc ccagcccgga cgcgatcgta tatgacaagt ggtatttaag
     2581 cgtgaaaacc ggtttccctt cccatatcgt tctggcagcc gctaccccat tgtcagcgcc
     2641 attcccaacc aggaataagc ttccgcccag gctttccggc gcagcataga tacctccggc
     2701 gatttcaagc ccactaccca acaattgcag cgtcccggta acccaatagt actctttagg
     2761 atatatataa tgcggaccca acgcctcggc gagatcagtg agctcactgg cgcttaattc
     2821 gcgacctttt ttttcttccg gcggtttctg gggtggagga gtggctttct ttttgggagg
     2881 tggcggcgga ggaggtggag gtggcgtagc aatttgagcg gacggcgtgg tcctgggcac
     2941 taccccgtag taggggccgg atgaccccca cacaccatcg gcaggagtac aattgtccca
     3001 cgtattagcg ttacattcgc tttgagtgtc gaattttttt tcttcgtatc catatttttg
     3061 cggaggcaaa tggctatagg catccgttcc gccgggatcg aataacttga ccggattgtc
     3121 gctgacataa gtatacatat tcaacccgtc cccgatcccg attggatccg ccgacgtcca
     3181 ccggcacaac cacagggcat aatacctcgc cccatggtaa ttcagcccgc tttcctcatc
     3241 ccgttccttc ccggtatacc ggtatcgttt ggcgggcacc tcgcgcgtgg catccagcgt
     3301 ggaatacgag gtggtaccat agggaaagta ttcttcatag gagatgatct tagccgcatc
     3361 atccagttcc agcgaagcag acccaagatg attctcgtag ttgtagcgga tcaattgagt
     3421 ctcgttgctg ccggccggtt ttaccaccgg ggtatcgacc atcgcaatac ggcgctgcgc
     3481 atccatcaca tgcagggtct cgcgttccaa tgcgatcgca ccagcgcttg ttttttgccg
     3541 gtagatctcg ataccgccga gataaatccg ttcattgctg gtcccgtcca ggcgttcaat
     3601 taccttccgg catctttgcc cggcgctatc gtaaacatag tataccgttc ccccgccgcc
     3661 gagatctgcc tgtttaaagt gatcggagaa atcccatagt tcgttcagca cggtttccag
     3721 gccagacatg ttaccgtgcg cgtcatagct ataatgaaaa gtatcggcgc cgagggtaac
     3781 atcggtcaga cggttgttgc cgttggtccc gccgtcatat gtaaagctgc gggtccagct
     3841 gctgtttttg gcgacatgtt gctgctgcaa caaatttccc gctttgtcat aggtatattg
     3901 ctcagtgtag ttccagaaag cattggcgtc gttggggttg atggaagtac tgtcgataaa
     3961 gggataaccg ccaaagggaa aactaccggg gatatccgat tttggccgta tgtccgtttg
     4021 gcccgcctgt ttgcggcccg tggcggaaac caatctatag acggcatcgt acacatattt
     4081 attcaaagcc tgcacctgct cgccatcgaa aaaaatatcc ggctgggcat tatcctttat
     4141 ctgcgtgata ttgccaaacg ggtcataggt atagttgaga tcctgcagga tatccgcccc
     4201 ggcattccgg gaagtcaata gacgcaccag ccggagcgtc tctttctggt aagtgtaccg
     4261 ggtaaccgta ttgttcccat aaaagatcgc ttcccgttgt cccttggcat cgtaatcaat
     4321 tttcgtaacg aacggggtgg gggtggccgc accccgtatt tttaccataa cagtatccag
     4381 cattccagcc tcattataac ccggggtgat ctcgctgggc ggcattgtca cggaatgggg
     4441 tgtgaccagt tttatgggtc ggttcaacgc atcgtattca gcagcgttct caaaaatctc
     4501 cggctccata tccacagggt tcgtccagtc gacggctgtt ttataattct ttgcaaactg
     4561 tttcgtcgct tgcaggggat tccccttgaa atcgaattgc cggctgatgc ttataccgga
     4621 ttgatcataa tttttgtaga ctttgccttt gacattatcc gcttttgcgg tggcgacggt
     4681 ttctccatat tcgatctgcg ccgcgatcgt ttcactcccg ccgcctgttt ttacaaagga
     4741 tgcgaccttt ctgcccagcg catcgtacgt aaaccgaaag gcatgatcac ggtcgtccca
     4801 tccaagcagc ggttttcccg ccacatcacc catcatccaa cggctgcccg cgtccatact
     4861 tgtttgcttc catttcattc cggtcatgcc gtatgcatac tccatcactt tccggtcaag
     4921 cggatccgtt gtttccagcg cgttgccgga aatgtccata atgatcctgc tggataattt
     4981 atcgccggcg ttgtcgacaa tcgtcaaaaa agtcctgccc agggaatcca ggtgtacgag
     5041 ggaaggggta tcggcgtccc tggccgcgag ccaggctgcc cttttttggg catcggccgg
     5101 ttccggatct gagggatccg gcttacccag ggcggcatac caatcgctgt ccttcaccgt
     5161 atcattgttg tcatacgaag cctgcagcca gggggtaaac tccaccctgg taaaagtgtt
     5221 gtcgggcaga tcggtgcgga taaccctgcc cggcggatcg taatgcatca caggagtaac
     5281 ccctaactgt accatttctt tttcatcgtc gaatgaggga tcgacgctaa aataaggctc
     5341 atattttttt acaggattgc ccttgttatt gatgatcgtc cgcccgttgc ccgcccatcg
     5401 cagattggga gaggtatcga tagtggtcac gcttccatcc gggtttattt gcaaggcggg
     5461 gcccggttcg gcctgggtct ttgagagcac gacatgccct gttccatcgg aataggcata
     5521 ggcttcccgg gtcccggagg aatgctgcgg atcggcatga taatgggttt ctctgacggt
     5581 cgtttttatc gtattcggct tgggcttgta gttggaaaga tcaaagccga ccgataaagt
     5641 ctggttatac cattcgaata ggtcatattc catttcactt cccggaaaat caacggcacc
     5701 ctttatctct gccctggagt cgtcgaactc gtcgcctgcg tcgacgccct tttttccgat
     5761 ggcgaatgtg ctcaccacta agcccagctc atcgaagcgg gctgccgtca ggttgtcatt
     5821 cgcgtcctgc atcagataag gcgtcaatac ccgatagttg aattttacca ccgaggtctc
     5881 attgccaagg gcatcggtcg tcttcgcgat aaaaagagaa tagttcggat cataggacag
     5941 gctggtctta ttgccaaagg gatccgtgaa aagcgtggac agataaaaat gagcggagtc
     6001 ataattcttt gttcccgaaa cagcccaata gtaattatcg ctgagaacaa aagcgccgcg
     6061 ggcggggtcc tgtagaataa cctggaggtc tgccggcgcg atcttcggac tgaagacggt
     6121 caacatatcc tggttgaagc aagcctggta agactggtca aacaaacctt tcgattccat
     6181 aatccccggc ggtaataccg tggtgccatc gttaccgcga tattgggtgc gggaaaattt
     6241 caatagtctt ttttgaatac cagtagtggg tgtaatattg aaatcgataa aagccgccgt
     6301 aatgcaatat tttttcaaat cttcccaggc gtagtgaaca actggtttag gcgcaccgga
     6361 gagttcatag gatcgggtgg tataaggcag cggcgcccgg tattccgcgt tggttcggat
     6421 gacattggtg aattgatttt cggtatatac gatatggagc tgctcttgtt cgggttcatt
     6481 gcccggcagc ggttgacggc catagaccac gctggcttgc tgcaggacat tgccaaaagc
     6541 atcgatcccg aatataaagc tatgggccat gcgcggatcg ccgggatccc gatcatagtg
     6601 gtaattgatc gcttcgcttt cgtggcaaag gaagacaccg tatttgtcgt ctccccgagg
     6661 ttgcagggtt tttatcacat aactgtgctg ctctgcacta tagggcttgt cttgcaatac
     6721 ggacgcgtcc agcgcatata tctcttttcg caatatcgag cctttgcagg cccggatggc
     6781 ctggcggcaa gtttccgcat cggagccgct gtccagggct gttgccggca aaagcttttc
     6841 cggcacgata ccattctgaa aatattcctt ttcgaatccg gccaggacct tg
//
LOCUS       DASYSV010000369         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2504619, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000369 DASYSV010000000
VERSION     DASYSV010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504619"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VGQ51_11330"
     CDS             complement(<1..917)
                     /locus_tag="VGQ51_11330"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEV9037209.1"
                     /translation="MDTRPTYDDIPWQLIIQALQHEETPEESVLFGDWLAASPANQAI
                     YQRLKQMWEEGLADYLIVRDADPEQAWVALQQLLDEPASEGEKTVILHPLAGKQPLRM
                     MRWLVAATLILVSGGIAMWYYQDKTVPIQYATIAGEQKTIPLPDGSTMVLQPETRLQL
                     PRTYNKTDRTVILQSGTVRFDVAHQEQLPFTVDLGVASVKDIGTTFTIVRNADSITVT
                     VTSGKIAFTEKSTGATRELSAGGALCLYTTTQHAGEVRVITPGGNSLRFDNATLQEVV
                     GALQRQFGKKILLEDTALAQKRVTLHLDG"
     gene            complement(925..1527)
                     /locus_tag="VGQ51_11335"
     CDS             complement(925..1527)
                     /locus_tag="VGQ51_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEV9037210.1"
                     /translation="MTPEEDNIWRSIQEKDRKAFEQYYKDHYKLFFVAAFKYLGDAGQ
                     AQEVVNDVFVTLWQNAEKVTIQSSLKAYLHRSVINRSLNLLDKNKRDRQQQQNTLQTE
                     EAAESREIETNELKLRLYKAIDQLPEQCRKVFRMSRFEQLKQQEIADRLGISIKTVKN
                     HITNALQQLKHVLDDWHNLPLWIIFFDIVTRSIGPFSCLI"
     gene            1587..2642
                     /locus_tag="VGQ51_11340"
     CDS             1587..2642
                     /locus_tag="VGQ51_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV9037211.1"
                     /translation="MKIGICFLIAINSLGVVWAQPEPRPSNGPSKTQLAQIGRKYGMF
                     IHFGINTFCDEEWTDGTRPPSVYRPSAIDADQWIATAKEAGMKYVILVTKHEDGFCLW
                     DSRYTTYDVGSSGNRTNVVEAVARACKRYGIGLGLYYSLWDRHLNPDPRDTTGDFDYN
                     QYMIAQLKELIGITHPYSPIVELWLDAGWEKQRSRWPIAAIYTVVHTLEPQCQVGVNW
                     SIGRPDNPDPDSSWVLPTEQRTGYPIRYFPSDFRMGDPYLPANPDPKLFSHDGKLYYM
                     PWETTVCMSGKWFYNTRDTVYKTADQLLRLYRIATAQDNILILDCPPDRTGRLREKDR
                     QLLFALRRLIDRPAQSQ"
     gene            2719..3153
                     /locus_tag="VGQ51_11345"
     CDS             2719..3153
                     /locus_tag="VGQ51_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV9037212.1"
                     /translation="MFSKTCEYAIRAMIFIALRSKDGDRVGIREIAKGIDSPAHFIAK
                     ILQQLVRKGVVQSVKGPNGGFYLDAQGRECPLAKIVRVVDGDQLFSGCGLGLRNCSEV
                     HPCPIHDEFKKIREDITAMLENSYIGEFTERLEENLLFLRRK"
     gene            3159..3542
                     /locus_tag="VGQ51_11350"
     CDS             3159..3542
                     /locus_tag="VGQ51_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEV9037213.1"
                     /translation="MDISNIEDVRVLVDAFYGKVREDRLIGPIFIGAIGDHWPEHLDK
                     MYRFWQTVLLEEHTYFGSPFAPHARMPLERRHFDTWLGLWDATVDELFAGVKATEAKW
                     RASKMAEMFLSKLEYYRNNPAKPIL"
     gene            complement(3578..4978)
                     /locus_tag="VGQ51_11355"
     CDS             complement(3578..4978)
                     /locus_tag="VGQ51_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEV9037214.1"
                     /translation="MKNFFGRIWHWLDDRMGIMDLLRPLIDHPVPPRGLWMYVFGSAT
                     LFMFILQVVTGVALSLLYQPSSDNAYHSLQFITYQAKFGNILRSVHFYGASAMIILVG
                     LHMIRTYITASYKFPREMNWISGVFLLLFTIFMGFTGQLLRWDSNGVWSSVVAAQQLG
                     RVPFFGKYLARLLLGGDTIGSHSLTRFFSYHVFIIPAFIFIFIGLHLWLVFKTGISEP
                     PKAGRPVDPKTYRKWYDDYLKKYAIPFWPNAAWRDVLFSSFIVIAIILFAWLKGAPDL
                     TTKPDMTYIHTSPQPDWYMVLFFALFALMPHKIESAAMTVGPLLTVIILFVIPFLSNK
                     GERSPIRRPWAMFGVICVFVFVISLLLLGLKHPWSADFDAKPLPVAAIHPMHPDATVV
                     AGSHLFYTRGCEYCHQVNGYGGKAGPDLSLVGLRLSAQEITIRIVNGGGNMPAYGGIL
                     NQDELATLVNFLSAQK"
     gene            complement(5003..5539)
                     /locus_tag="VGQ51_11360"
     CDS             complement(5003..5539)
                     /locus_tag="VGQ51_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV9037215.1"
                     /translation="MNKEELTHLPSYNPDNREEMTRKEFMTRVSLSIAAISAVVMAVP
                     CVAALVAPLLESNPSSWRTVGRLEDFPVGSTRLVNFENADAEPYAGMLTKSAAWLRRV
                     DQEKFTAFAVNCTHLGCPVRWEETAGLFMCPCHGGVYYNDGTVAAGPPPRPLPEYKVR
                     VHNNEIQLETAPLPITGV"
BASE COUNT         1278 a         1448 c         1559 g         1277 t
ORIGIN      
        1 tctccatcca ggtggagggt cactcttttt tgagccagag cggtgtcttc caggagtatc
       61 tttttgccga attgccgttg taaggcgcct acgacctcct gcagcgtggc attgtcgaac
      121 cgcaggctgt tgccgccggg agttatgacc cttacctcac cggcatgttg tgtcgtcgta
      181 taaagacaca gcgcgccgcc ggccgataat tcccgggtcg cgcccgtcga cttttcggta
      241 aaggcgatct ttccgctggt tacggtcacc gttatgctgt cggcattcct gacgatggtg
      301 aaggttgttc cgatatcttt tacgctggct actccgagat cgaccgtgaa gggcagctgt
      361 tcctggtgag cgacgtcgaa tcggacggtt ccgctttgca ggatgacagt tctgtcggtc
      421 ttgttataag tgcggggcag ttgaagacgg gtctccggct gaaggaccat cgtcgaacca
      481 tccggcagag ggatcgtttt ttgttcaccg gcgatcgtcg catattggat cggcaccgtt
      541 ttgtcctgat agtaccacat tgcgatacct cctgaaacca ggatcagcgt cgccgcaacc
      601 agccatcgca tcatccggag cggctgtttt ccggccaggg gatgcaggat gacggttttc
      661 tctccttcgg acgccggttc atccaataat tgctgcaaag caacccaggc ctgttcggga
      721 tcggcgtccc ggacaatgag ataatcggcc agtccttcct cccacatctg tttcagccgt
      781 tggtaaatgg cctgatttgc gggggatgcg gccagccaat ccccgaacaa tacgctttct
      841 tcaggcgtct cttcgtgctg aagagcctga atgatcagct gccagggaat atcatcatat
      901 gtcgggcgcg tgtccatgat ctacttatat aagacaggaa aatggtccga tggacctagt
      961 gacgatatca aaaaaaatta tccatagggg tagattgtgc caatcgtcga gtacatgctt
     1021 cagctgctgc aaggcgttgg taatatgatt cttgactgtc ttgatggaga tgcccagacg
     1081 atctgcgatc tcctgctgct tcagctgctc gaacctgctc atccggaaga ctttgcggca
     1141 ttgttcgggg agctgatcga tggccttata aagacggagc tttaactcgt tggtttctat
     1201 ttcccgcgat tcggcggctt cttctgtctg cagcgtgttt tgctgttgct gccggtcgcg
     1261 tttgttctta tccaggaggt tgaggctccg gttgatcaca cttctgtgga gataggcctt
     1321 cagcgaagac tggatggtga ccttttccgc attctgccaa agcgtgacga atacgtcatt
     1381 gacgacctcc tgcgcctggc cggcatctcc gagatatttg aaggccgcca cgaagaagag
     1441 cttatagtgg tccttataat attgttcgaa agcctttctg tctttttcct gtatggatct
     1501 ccagatattg tcttcttcag gggtcatact tcgaggcgat ttgaaaatag tggtggtttc
     1561 gaagcaatat taataaattt attcccatga aaatcggcat ttgctttctc atcgcgatta
     1621 attcgctggg cgtcgtttgg gctcagcccg aaccaaggcc gtccaacggc ccgagcaaga
     1681 cgcagctcgc acagatcggg cggaaatatg ggatgttcat ccattttggc atcaatacct
     1741 tttgcgacga ggaatggacg gacggcacca gaccgccatc cgtctaccgg ccttccgcga
     1801 tagacgccga ccagtggatc gccacggcga aagaggcggg aatgaagtac gttatcctgg
     1861 tgaccaaaca tgaagatggt ttttgtctct gggacagccg atatacgact tacgatgtcg
     1921 gaagttcggg gaatcgcacg aatgtggtag aggcggttgc cagggcatgc aaaagatacg
     1981 gcatcgggct gggcctttat tattcgcttt gggaccgtca tctaaacccc gatccgcgcg
     2041 acacgacggg agatttcgat tacaaccagt atatgatcgc gcagctgaag gaactgatcg
     2101 gaatcactca tccctattcg cccatagtcg agctatggct ggacgcgggt tgggaaaaac
     2161 agcggtcacg ctggccgatc gccgccatct atacggtggt ccataccctc gagccgcaat
     2221 gccaggtcgg cgtcaactgg tctatcggaa gacccgacaa ccccgatccc gattcaagct
     2281 gggtgctgcc cacggaacaa agaacagggt atccgatccg ctacttcccc agcgatttcc
     2341 ggatggggga cccttatctg cctgccaatc ccgatccaaa gctgttctca catgacggaa
     2401 agctctatta catgccctgg gaaacgacgg tgtgcatgag tggcaaatgg ttctacaaca
     2461 cccgggatac cgtttacaag acggcggatc agctgctacg gctgtacagg atcgctacgg
     2521 cacaggataa tatcctgatc ctcgattgcc ccccggaccg gaccggaagg ttacgtgaaa
     2581 aagaccggca gttgctgttc gcgctgcggc gtttgatcga ccgaccggcg caatcacagt
     2641 agaccccttt gccgcactcc ggccaaataa aagacattct tgtcttttat ttgaaaattg
     2701 cctcgtatct ttgcggcgat gttttccaag acatgcgaat atgcgatacg ggcgatgatc
     2761 tttatcgcct tgcgttcgaa agacggcgac cgggtcggga tacgggaaat cgccaagggc
     2821 atcgactcac ccgcgcattt catcgccaag atccttcagc agctggtgcg aaaaggtgtt
     2881 gtccaatcgg taaaggggcc caacggcggg ttttatctgg atgcgcaggg tagggagtgt
     2941 ccgctggcga agatcgtccg ggtagtggac ggcgatcagc tgttctccgg ctgcggactt
     3001 gggctccgga actgttcgga agtacatccc tgtcccatac atgatgaatt caaaaagatc
     3061 agggaggaca ttaccgcaat gctcgagaat tcatatatcg gggaattcac ggaaaggctg
     3121 gaagagaacc tgctgttcct ccggcgtaaa taaatcatat ggatatttct aatatagagg
     3181 atgtgcgtgt gctggtggac gccttttatg gtaaagtgcg ggaagaccgg ctgatcggcc
     3241 ccatcttcat cggcgccatc ggagatcact ggcctgagca tctcgacaaa atgtatcgct
     3301 tctggcaaac ggtattgctg gaggagcata cctatttcgg cagtcccttt gcacctcacg
     3361 cgcggatgcc gttggaaaga cggcatttcg acacctggct cggtctctgg gatgcgaccg
     3421 tggacgagct tttcgcgggc gtcaaggcaa ccgaagccaa atggcgggca tcgaagatgg
     3481 cggagatgtt tctttcgaag ctcgaatact accgcaacaa cccggcaaaa ccgatcctat
     3541 gacgacgacc cctttgcgtg cggtaaccgg atctgtctca tttctgtgcg cttaaaaagt
     3601 tcactagcgt ggccagctca tcctggttga gtataccacc gtacgccggc atgttgccac
     3661 cgccgttgac gatgcggatc gttatctcct gcgcggacag acgaagaccc accaggctga
     3721 gatcggggcc cgccttgccg ccatacccgt tcacctggtg acaatattca caacccctgg
     3781 tataaaaaag atgagaaccc gccacgaccg tagcgtcggg gtgcatcgga tgaatagctg
     3841 cgaccggcag tggtttggcg tcgaaatccg cggaccaggg atgtttcaga ccaagaagca
     3901 gcaggctgat gacgaatacg aagacgcaga tgacaccaaa catcgcccag gggcgccgga
     3961 tgggactgcg ttcgccctta ttcgaaagga agggaatgac gaacaggatg atcaccgtca
     4021 ggagcgggcc gaccgtcata gcggcggatt cgatcttatg cggcatcagc gcgaacaagg
     4081 cgaaaaagag gaccatatac cagtccggct ggggtgaggt atggatatac gtcatatccg
     4141 gtttcgtcgt caaatccggc gcacctttca accaggcaaa caggatgatc gcgatgacga
     4201 tgaagctgct gaacaggaca tcccgccagg ctgcgttcgg ccagaaaggg atcgcatatt
     4261 tcttcaggta atcatcgtac cattttcgat aggtcttagg atcgaccggc cttccggcct
     4321 ttggtggctc ggatatcccc gtcttgaaca ccagccagag atgcaggccg ataaaaataa
     4381 aaatgaacgc gggaatgatg aatacgtgat aggaaaaaaa tcgggtgagg ctgtggctgc
     4441 cgatggtgtc tccgcccagc agcaacctgg cgagatattt cccgaagaaa ggaactcttc
     4501 ccaattgctg ggccgctact acggaagacc agacgccgtt ggaatcccag cgcaacagct
     4561 gcccggtgaa ccccataaag atcgtgaaaa gcagcaggaa tacgccgctg atccagttca
     4621 tttcgcgtgg gaatttgtac gaggcggtga tatacgtccg gatcatatgc agaccgacca
     4681 ggatgatcat ggccgatgca ccgtagaaat ggacgctgcg aaggatgttc ccgaattttg
     4741 cctggtaagt gatgaattgc aacgagtgat aggcattgtc cgaagaaggc tggtacaaca
     4801 gcgaaagggc tacacctgtg acgacctgca aaatgaacat gaatagggta gcgctgccga
     4861 aaacatacat ccacagcccg cgcgggggaa cagggtgatc gatcagcgga cgcaggaggt
     4921 ccatgatgcc catgcggtcg tcgagccagt gccatatccg tccaaagaaa ttcttcattt
     4981 gaataaatgg ttttggttat ctctagacgc cggtgatggg gagcggagcg gtctccagtt
     5041 gtatctcatt gttatggacc cgtactttat attccgggag gggtcgtggc ggcggaccgg
     5101 cggcaactgt tccgtcgttg taatacacgc caccatggca gggacacatg aagagccccg
     5161 cggtttcttc ccatcttacc ggacaaccca gatgcgtaca attcacggca aaagcggtga
     5221 acttctcctg gtcgacgcgc cgcagccagg cggcggattt ggtcagcatg ccggcataag
     5281 gctccgcatc ggcattttca aaattgacca gacgcgtact gccgacggga aaatcttcca
     5341 ggcgacccac cgtccgccag gagctgggat tgctttcgag aagcggagcg acgagcgcgg
     5401 cgacacatgg tactgccatg acgaccgcac tgatggcggc gatactgagg ctgacacggg
     5461 tcatgaattc ctttcgcgtc atttcctcgc gattgtccgg attgtaagat ggcaggtgcg
     5521 ttagttcctc tttattcatg gcgtagaata tttatccatc cg
//
LOCUS       DASYSV010000370         6004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_73101, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000370 DASYSV010000000
VERSION     DASYSV010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73101"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2597
                     /locus_tag="VGQ51_11365"
     CDS             <1..2597
                     /locus_tag="VGQ51_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEV9037216.1"
                     /translation="AGRYLTGPLFLRSHLTIEIDPGAVLLFQTDIRHTPAMDGSWEGL
                     DRKVYASLFTGFDLEDITITGRGRLDGQGKVWWDAARQTMEMRKKFHLVEREPENPPG
                     SLLQWGRPKMIDLYRCRNVEISGVTITNSPSWTIHPIYCKDVDIHGVRIIQPYDSPNT
                     DGIDPESCSDVRITGCYIDCGDDCIAIKSGYNASGRAKGIPCQNVVISNCTFAHGRSA
                     VGIGSEMSGGVKNVTVTGCVFHGTLRGLRVKTERGRGGVVEGLIADDIIMDSVSEGIS
                     IDMYYDGHDTAFRPVTVETPFFRNIRYFNIIGRHVKHAISIHGLPEAPVDGITLENVF
                     MDADDAGDCEFVHDLSLINLELKIQDRPATIRLDHCPGAVIRDIRADGEAEKVHGYRH
                     LPGAQSSPDYEVSVNEQPMFVEKIKKFDVPVHFTSFAFDGPAQVEIACHEKISTYSVS
                     TAGPQIHASTRGNNLVIRLDRPQYFVVKINDLQYLFVAAELPESGGGGRHAIDVSTLG
                     VSGSGGDLQTERLQKILDSLSALGRPSTLYFPAGLYRTGQLNMRNHVSLYLDDGALIK
                     GSTDPRDYRESLIRMDHISGVSISGRGAIDGAGWDGLRRNGGKGLYLLYLSDCKGINI
                     EGVQLRDPCFWNTRIFRCSNIRMRDIKILNDRPERNWVNTDGIDFDSSTGGDVYNVMM
                     MTGDDNIVVKGLETRASYNPAHIRFDRVIGLSNSAAAKIGTETRTPLIDDIEFNHIDI
                     VQCKRAIVISGFDSAAIRNIVFRDIRVEKVVFQGNEEPRIIDFEITDHSWRDCVGRCS
                     ISHVLIDGITIPFSLDGVSSQILGRTPEFGVSDVEIRGFWVMGKRVTSMKDAHLVENQ
                     YVSGVRFE"
     gene            2619..4523
                     /locus_tag="VGQ51_11370"
     CDS             2619..4523
                     /locus_tag="VGQ51_11370"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEV9037217.1"
                     /translation="MIKKILLPAVVCWSAMSVAGQPSTGKFEPVDFAQVRIDDSFWSP
                     ILDRLRTVTLRACIEYTETKTGRIRNFEKAAMRKGQHEGKYYDDSDVYKALEAIGYVL
                     HMGRDSALERTADEWIDKIAAAQLSDGYINTYYTLTGIDHRWTDMEKHEDFCAGHLIE
                     AGIAYYKATGKRKLLEVGIRMADHMDRTFRLSNRHWVSGHEEIELALVKLYHLTHNVS
                     YLKLSEWFLDQRGRGYGKGVIWDDWKKPGYCQDDVPVKAQRTIEGHAVRAMYLYSGAA
                     DVESATGDSSYWAALESVWEDVVGRNLYITGGIGAEESNEGFGLPYHLPNRDAYCETC
                     ASVGMVFWNQRMNSLSGDAKYADVLERSLYNAALDGISLSGDHFFYGNPLASSGDVTR
                     KEWFGTACCPSNIARLLASLGGYIYGASSKAIWVNLFIQDSASIPMGADTLGITQHTR
                     YPWDGAVELQVSPEKETNFELHIRIPGWLDRPVPGGLYAYANQGDQRPELYINGGKAD
                     YKLEQGYAVLARKWKKGDRVDLRFPMKVRMIEANPFVKADSGRSSLQYGPLVYCIESV
                     DNSQPVQSMSVRPEGLFTVQYEPHFPGGVNVIRWATSANGKKSEALAIPYYSWNNRGP
                     GQMEVWLLAD"
     gene            complement(4520..5800)
                     /locus_tag="VGQ51_11375"
     CDS             complement(4520..5800)
                     /locus_tag="VGQ51_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV9037218.1"
                     /translation="MARNTSNLTFLRLFAALALILPLTSLAQTVAPATDKGVKFEDNL
                     SWSAIRAKAKAENKYIFMDCYTTWCGPCKYMAKTIFPQEETGAFFNDKFICVGVQLDT
                     TAKDDDHVKSWYADGHAIAEEFDVRAYPTYLIFSPDGKPLHRLVGSSATAQKFISHMQ
                     STFDSTLQYYTQLQEYRDGRRDSAFLFRTAKMCANVYDLKTGHEVADAYFATQGDPYS
                     RGVLELTLQFTQSTKDKGFPVIVNHPDKVDAVMGNGAAEEAMFGIFYRSTIYPAIRKA
                     GSGTPDWKAIRKSIAAEFPSIAGEMTEKAQLVYYQQKKDWPHFQTSVVSYMQKYGAHV
                     NAGDLNNFAWTVFQNCPDMTCVSEALEWSKRSFEDKPNSMFMDTYANILYKMGKKDDA
                     IVWEQKAIDIADEGSKADMQATLEKMKKGEKTWD"
BASE COUNT         1363 a         1353 c         1884 g         1404 t
ORIGIN      
        1 ccgcgggccg gtacctgacg ggcccgctat ttctgcggag tcatctgacg atcgagatcg
       61 acccgggggc cgtacttttg ttccagaccg acatccgcca tacgcctgct atggacggca
      121 gttgggaagg attggacagg aaagtgtatg cgtccctgtt cacaggattc gatctcgaag
      181 atatcacgat aaccggcagg ggcaggctcg atggacaggg aaaggtctgg tgggatgcgg
      241 cgcggcagac catggaaatg cgaaagaagt ttcatttggt ggaacgcgaa cccgagaatc
      301 cgccgggaag tcttttacaa tggggcaggc ccaaaatgat cgatctttac aggtgccgga
      361 atgtggagat ctccggcgtg acgatcacca attctccttc ctggacgatc catcccattt
      421 actgcaagga cgtggacatc catggggtga ggatcatcca accttatgat tcgccgaata
      481 cggacggtat tgatccggag tcttgttccg acgtaaggat caccggttgt tatattgact
      541 gcggcgatga ttgtatcgcg atcaaatcgg gttataacgc ctcgggcagg gccaaaggca
      601 ttccctgtca aaatgtggtc atttcgaatt gcacgtttgc gcatggccgg agtgcggtcg
      661 gcatcgggag tgaaatgtcg gggggcgtaa agaacgtaac ggtcactgga tgcgtttttc
      721 atgggaccct gcgcggtctg cgtgtaaaaa cggagcgagg caggggcggc gtcgtggaag
      781 gattgatcgc ggatgatatc attatggaca gcgtctcgga gggtatctcg atcgatatgt
      841 actatgacgg acatgatacg gccttccggc cagtgaccgt ggaaacgccc ttcttcagga
      901 atatccggta tttcaatatt atcggccgtc atgtgaagca tgcgatctcg atccatggat
      961 taccggaggc gccggtggat gggatcacgc tcgaaaatgt attcatggat gcggacgatg
     1021 caggggactg cgaatttgtc catgatctta gcctgatcaa tttagaattg aaaatccagg
     1081 atcggcccgc gacgatacgg ttggatcatt gtccgggcgc ggtgatccgc gacattcggg
     1141 cggatggtga agcggagaag gtgcatggtt accgacattt accgggggcg cagagtagtc
     1201 ccgattatga agtcagcgtc aatgagcagc cgatgttcgt ggaaaagatc aaaaagtttg
     1261 atgtgcccgt tcattttacc agttttgcat tcgatgggcc ggcacaggta gagatagcct
     1321 gccacgaaaa gatctcgacc tattcggtaa gcacggctgg accgcagatc catgcgagta
     1381 cccgggggaa caacctggtg atccgtctcg accggccgca atattttgtg gtgaagataa
     1441 acgatcttca ataccttttt gtggcggctg agctgccgga gtccggcggg ggcggccgtc
     1501 atgcgatcga tgtatccacg cttggtgtaa gcggctcggg gggcgatctt cagacggagc
     1561 gactgcaaaa gatactcgac tcactttctg cgctgggacg tccatcaact ctgtatttcc
     1621 ccgcggggct ttatcgaacc gggcaactga atatgcggaa tcatgtttcg ttgtatttgg
     1681 atgatggcgc gctgatcaag ggtagtacag acccgcgcga ctaccgggag agtcttatcc
     1741 gaatggatca tatatcgggc gtctctatct cgggtcgcgg cgcgatcgat ggagccggtt
     1801 gggatggact gaggaggaac ggcggcaagg gattgtatct gttgtatctt tccgattgca
     1861 aggggatcaa tatcgaaggc gtacagctga gagatccctg tttctggaat acccgtatct
     1921 tccggtgcag caacatccgg atgcgggaca tcaagatcct caatgaccgt ccggagcgca
     1981 attgggtgaa taccgacggc atcgatttcg actcatccac cggcggagac gtgtacaatg
     2041 tcatgatgat gacgggtgat gacaatattg tcgtaaaagg attggaaacg cgggcgagct
     2101 acaatcccgc gcatatccgt tttgaccggg tgatcgggtt gagcaactcg gccgccgcga
     2161 agatcgggac agaaacaagg acacccttga tagacgacat cgaattcaac catatcgaca
     2221 tcgttcaatg caagcgggcg atcgtcatca gcggatttga cagcgccgcg atacggaata
     2281 tcgtcttcag ggatatccgg gtggaaaagg tggtctttca ggggaatgaa gaaccacgga
     2341 tcatcgattt cgagataacg gatcacagct ggcgggattg cgtgggccgg tgcagcatct
     2401 cccatgtcct gatcgatggc atcacgatcc cgttttcgct ggatggggta tcttcgcaga
     2461 tcctggggag gacccctgaa tttggcgtaa gtgatgtcga gatccgcggg ttctgggtca
     2521 tgggaaagag ggtaacctcc atgaaagacg cgcatctggt ggaaaaccaa tacgtgtcgg
     2581 gggtgcggtt tgagtaggct taaaaaaatt gcgaaaagat gataaaaaaa attttgctgc
     2641 cggcggtggt ttgctggtct gcgatgagcg tcgcggggca gccatctacc gggaagttcg
     2701 agccggtgga ttttgcacag gttagaatcg acgattcgtt ctggagtcct attctggatc
     2761 gtttgcgcac ggtgacgctg agggcctgta tcgaatatac ggaaacaaag acgggcagga
     2821 tccgcaattt tgaaaaagcg gcgatgagaa aaggacagca tgaagggaaa tattatgatg
     2881 actcggacgt ctacaaggcc ttggaagcca tcgggtacgt attgcatatg ggcagggact
     2941 ccgccctgga gaggacggcc gatgagtgga tcgacaagat tgccgctgcg cagctgtctg
     3001 acggttatat caatacgtat tacacgctga cgggtatcga tcatcgctgg accgatatgg
     3061 agaagcacga ggatttttgc gccggccacc tgatcgaggc gggcatcgcg tattataagg
     3121 ctaccggcaa acgtaagctc ctcgaggtgg gtatccgtat ggccgatcat atggatcgga
     3181 cgttcaggtt gagcaacagg cattgggttt cgggccatga ggagatcgag ctggctctcg
     3241 tcaagttgta tcatctcacg cataatgtct cctatctcaa actgagcgaa tggttcctgg
     3301 atcaacgcgg acgtggctat ggcaaggggg tgatctggga cgattggaag aagccgggct
     3361 attgccagga cgatgtaccg gtgaaggctc agcgcacgat cgaaggccat gccgtaaggg
     3421 cgatgtatct gtattcggga gccgcggatg tggaatccgc caccggtgac agctcctatt
     3481 gggcagcgct ggaatccgtg tgggaggatg tagtaggcag gaatctttat ataacggggg
     3541 gtatcggagc cgaagaaagc aatgaaggat tcgggcttcc ttatcatctt ccgaaccgcg
     3601 acgcgtattg cgaaacctgc gcttcggtag gaatggtatt ctggaatcaa cggatgaata
     3661 gtcttagcgg ggacgctaaa tatgcggatg tattggaacg gtcgttgtat aacgcggccc
     3721 tggatgggat ctcgcttagc ggtgatcact tcttttatgg caacccgctg gcatcttcgg
     3781 gtgatgtgac ccggaaggaa tggttcggga ccgcctgctg tccgtcgaat attgccaggc
     3841 tgctggcttc gttgggaggg tatatttatg gcgcctcgtc caaagctata tgggtgaacc
     3901 tcttcattca ggattccgcg tctataccga tgggcgcgga cacgctcggg ataacccagc
     3961 atacccgata tccttgggat ggcgcggttg aattgcaggt cagtcctgaa aaggagacaa
     4021 atttcgaatt gcatatccgc ataccggggt ggctggacag gccggttccg ggaggacttt
     4081 atgcgtatgc aaaccagggt gatcagcggc cggaattgta tatcaatggc gggaaagcgg
     4141 attataagct ggagcaggga tatgccgtgc tagcacgaaa atggaagaag ggagacaggg
     4201 ttgatcttcg atttcccatg aaggtgagga tgatcgaggc taatccgttt gtaaaggccg
     4261 actcgggaag atcttcgctc cagtatggac cactggtcta ttgtatcgag tcggtggata
     4321 attcgcagcc ggtgcaaagt atgtcggttc ggccggaggg gctgttcact gtgcaatatg
     4381 agccgcattt tcccggaggt gtcaatgtga tccgatgggc tacatccgcc aatggcaaga
     4441 agagtgaggc attggccata ccttattatt cctggaataa ccgggggccg gggcagatgg
     4501 aagtatggtt gcttgctgat tagtcccagg tcttctcgcc tttcttcatc ttctccagtg
     4561 tagcctgcat gtcggctttg ctgccctcat cggcgatgtc gatggccttc tgctcccaaa
     4621 cgatggcgtc gtccttttta cccatcttat agaggatgtt ggcataggta tccatgaaca
     4681 tggagttggg tttgtcttcg aaagaccgtt tgctccattc gagggcttcg ctgacacaag
     4741 tcatgtcggg acagttctgg aatacggtcc aggcgaaatt gttgaggtcg ccggcgttga
     4801 catgcgcgcc gtatttctgc atatagctaa cgacgctggt ctggaaatgc ggccagtcct
     4861 ttttctgttg gtagtacacc aattgggctt tttccgtcat ctctccggct atggaaggga
     4921 attcggcagc gatcgatttg cggatcgctt tccagtcggg agtgccggag cccgccttgc
     4981 ggatggcggg gtagatggtc gatctgtaga agatgccaaa catcgcctct tcggccgcgc
     5041 cgttacccat gacggcatcc actttgtcgg gatggttgac gatgacggga aagcctttgt
     5101 cctttgtact ttgggtaaat tggagcgtga gttcgagtac gccacgcgaa tagggatcac
     5161 cctgggttgc gaaataggca tcggcgactt catggcctgt cttcagatcg tacacgttgg
     5221 cgcacatttt cgctgtccgg aagaggaagg cgctgtcgcg gcggccatcg cggtattcct
     5281 gcagctgggt ataatactgg agagtcgagt cgaaggtgga ctgcatgtgg ctgatgaatt
     5341 tttgagccgt ggcgctcgaa cccaccaggc gatgcagggg tttgccgtcg ggtgaaaaga
     5401 tcaggtaggt gggataggcg cggacgtcga attcttcggc aatagcatgt ccgtcggcat
     5461 accaggactt tacatgatcg tcgtctttgg cggtggtgtc gagctgaacg cctacgcaaa
     5521 tgaatttatc gttgaagaag gcgcctgttt cctcctgcgg gaaaattgtc ttagccatgt
     5581 atttgcaggg gccgcaccag gtggtgtagc aatccatgaa gatatatttg ttctccgctt
     5641 tggctttggc ccgaatggcg gaccaggaaa ggttgtcttc gaattttaca cctttgtccg
     5701 tcgcgggtgc gacggtctgg gcaagcgagg tgaggggaag gataagtgcg agggccgcga
     5761 aaaggcggag gaaggtcagg tttgaagtat tgcgcgccat aggagtcaat tttgtacaag
     5821 atagtgtggt ggcgccagat gggcaaaaaa ttgggatagg cgggtcgggg gcgcggaagt
     5881 atagcagcgg agcggggacg cggaatctta gcggcgggag ggtgaaaaga acggcaaatc
     5941 tgagtcagtt tgaacccagg cccggagtcg aagcagtacc ggacctcggc gaactgttaa
     6001 aaaa
//
LOCUS       DASYSV010000371         6229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1870160, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000371 DASYSV010000000
VERSION     DASYSV010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870160"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..632
                     /locus_tag="VGQ51_11380"
     CDS             138..632
                     /locus_tag="VGQ51_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEV9037219.1"
                     /translation="MSELLNNWEKKAAERQKIYKRWLEKADRAPKRNRILEALPEAHE
                     EGFRAVNCLECANCCKRYSPRFKTPDIKRISRHLRLKESEFIEQYLRLDEDGDYVAKT
                     TPCPFLGADNLCSIYEVRPSDCERFPYTDEDVLVKRPALTLKNSTFCPIVYVVLERLM
                     KAVP"
     gene            complement(629..1525)
                     /locus_tag="VGQ51_11385"
     CDS             complement(629..1525)
                     /locus_tag="VGQ51_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV9037220.1"
                     /translation="MHRRIFLQQSSLALGALSLPGKALFHQDPWKMRILRNNVGIFYE
                     KGGTIAYYLSPEGIVVVDAEFPEQAGHLIAELKKQSDKPFAMLINTHHHGDHTGGNIA
                     FKGIVAHVAAHTNSLSNQQRVATTQHTEDKQLYPDITYGDDWEYKIGDEKIKTTYFGA
                     AHTDGDSIIHFTNANIAHMGDLMFNRRWPFIDRSAGASIASWIAVLDRSLKKYDNDTL
                     FIFGHAFDPDKVTGNKDDLRAMKDMLSRLQDFAAGWVKAGKTKDDLLKEKAIPGVTEW
                     QGDGIQRPLQAAYEEATAAPRS"
     gene            complement(1549..2790)
                     /locus_tag="VGQ51_11390"
     CDS             complement(1549..2790)
                     /locus_tag="VGQ51_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9037221.1"
                     /translation="MTEQRKLTALEILKIPEYRNFILARFFYVMALRMVTTIVGWRIY
                     EITRNPFAIGLIGLSEFLPAFCLALYAGHVIDKSDKRALLLKTTTTYLLCVIGLLALS
                     FGPLTRSLQSHWIQWLTYTIIFCTGVIRAFAGPALSAIIAQIVPKEKLPAAVTLNTTA
                     FLFASVTGHATAGFLIAHSSYLFCFSVVGVYVLIAWAFLFSVGPKPIMASGDRRTWES
                     IKEGLRFVFHTKEVLGALALDLFAVLFGGAVAMIPVYARDILKVGPIGFGWLNAASDI
                     GSIVMMIALTLRPLHRRQGYVLLYAVAGFGLCIIVFGVSHIFLVSYVALICSGMLDGI
                     SVIIRGTIVQVKTPDPMRGRVSAVNSMFINSSNELGQFESGVGARLMGVIPSVVFGGC
                     MTLAVVVVTWFKAPSLRKMEY"
     gene            complement(2796..3428)
                     /locus_tag="VGQ51_11395"
     CDS             complement(2796..3428)
                     /locus_tag="VGQ51_11395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEV9037222.1"
                     /translation="MKYHFITIEGNIGAGKTTLAHLLARHYNARLILEQFADNPFLPK
                     FYENPGQYAFPLELFFMAERYKQLKELVHAGDLFQSLTISDYLFTKCLLFARVTLAEQ
                     EFRLYQKFFEILESQIIQPELVIYLHAPVSKLQANIRKRNRPYEQSIPDDYLLRIQET
                     YTQYIRQHNTPTLFVDVSNADFLGNERHIRVILDALEGHRGEGQQYLTLP"
     gene            complement(3425..3922)
                     /gene="folK"
                     /locus_tag="VGQ51_11400"
     CDS             complement(3425..3922)
                     /gene="folK"
                     /locus_tag="VGQ51_11400"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV9037223.1"
                     /translation="MNISYLLIGGNEGDRENRLAEARRQIMEMAGPIRKTSSLYETAP
                     WGNLHQPWFLNQALQVETTFDAATLMTSLLGIEEKMGRKRLEKYGSRAIDIDILFFND
                     AIVAEPGMIIPHPEIANRRFVLEPMDEIAPDYIHPVLGVSVQQLLQTCTDPLEVKRLD
                     AKRGA"
     gene            3990..5747
                     /gene="sppA"
                     /locus_tag="VGQ51_11405"
     CDS             3990..5747
                     /gene="sppA"
                     /locus_tag="VGQ51_11405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEV9037224.1"
                     /translation="MRGFFKTFFAALLALAVFTVLAVFILMGVVGGILSPGRPDVGGK
                     GVLVVDLDQSYKEQVQDNPLSDLGSDDQYDVPGVYDVVRLLKHAAGDSAIKGIYLKCN
                     GDPNGFATNEEIRKALVGFRKSGKFVYAYGEGISQKAYYVASMADRIYCNPKGDLEWK
                     GFSAEMLYLKGALKKLEIDPQIFYAGKFKSATEPLREDHMSDANRIQLTQLLGDFYNR
                     LLRTTAESRGLDTAVLRKCANEHLIVRAADALQYRLVDGLKYDDEVKDELRSKLGVVA
                     HGWINFVPLGKYAKSVDFRKVGKDRIALIYAEGDVVDGKGERDEIGSDTYRWLIRKAR
                     FDKDIKAIVIRVNSGGGSSLASENIWREVELAKKDKPVIVSFGDVAASGAYYLSCNAD
                     SIFALPGTITGSIGVFTIIPNLKSFFNNKLGITFDGVKTAPEADLLSTTRPLTPSERA
                     LVQNMVDTIYSTFKGRVADGRKMGLGYVDSIAQGRVWSGTAGSELGLVDRIGTLQDAV
                     DCAARMAGTSDYHLVEYPEPRSFLDRLLGTYKHSTSMKTAVREEIGEENYRSWMTLKK
                     LKSMIGVSETKLPFDLTIE"
     gene            5772..>6229
                     /locus_tag="VGQ51_11410"
     CDS             5772..>6229
                     /locus_tag="VGQ51_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037225.1"
                     /translation="MATNYSQLRGMLAIAKGSFKAILRNPSAVAFSFGFPLVFILVFG
                     FIGGGGPSVSVALADPRDSATAIVRGLLNSKMVKLSDDRDSVTIRKDLERGRIAAVIS
                     FDSAKSPAGFMQYTVHTQTSSASGDKYPILRLALAQGFERAVEGTMPKEY"
BASE COUNT         1390 a         1502 c         1921 g         1416 t
ORIGIN      
        1 tccgatgatg ataaggtata gcggactgct gatcaggatc agtatttgtt ctcggaggtc
       61 ttccacgggc taaaaataag gtaaataatg cacctttgca gcggggcaag ttgttaaaag
      121 tatgcaaagg gttatgtatg agtgaattat taaataattg ggaaaaaaag gccgccgagc
      181 ggcaaaagat ctataaacgc tggctggaga aagccgacag ggcgccaaag cggaacagga
      241 tcctggaggc gttgcccgag gcgcatgaag aggggttccg ggccgtcaat tgtctggagt
      301 gcgccaattg ctgcaagcgg tattctccgc ggttcaagac cccggatatc aagcggatct
      361 cgcgtcatct ccggctgaag gaaagcgagt tcatcgaaca atatctcagg ctcgatgagg
      421 acggggatta cgtagcgaag acgacgccct gtcctttcct gggcgctgac aatctttgca
      481 gcatctatga ggtccggcct tcggattgcg agcggtttcc ttatacggat gaggatgtgc
      541 tggtgaagcg gccggcgctg accttgaaga attctacatt ctgtccaatc gtttatgtgg
      601 tgctcgagcg actgatgaag gcggtgcctt agcttcgcgg cgccgccgtt gcttcttcgt
      661 aggctgcctg caggggtctt tggataccgt cgccctgcca ttcggtgacg cccgggatgg
      721 ctttttcctt tagcagatcg tctttggtct tgccggcttt gacccagccg gcggcgaaat
      781 cctgcagccg ggagagcatg tccttcatgg cgcggaggtc gtccttattg ccggtgacct
      841 tgtcggggtc gaaggcgtgg ccgaatatga agagcgtgtc gttgtcgtac tttttcagcg
      901 atctgtcgag taccgcgatc caactggcga tcgacgcgcc ggcgctgcga tcgatgaagg
      961 gccagcgtcg gttgaacatc aggtcaccca tgtgtgcgat gtttgcgttg gtgaagtgaa
     1021 tgatgctgtc gccgtcggta tgggcggcgc cgaaataagt ggtctttatc ttttcgtctc
     1081 cgatcttgta ttcccaatcg tcaccgtagg tgatgtcggg atagagttgt ttgtcctccg
     1141 tatgttgtgt cgtggcgacc cgttgctggt tggatagcga gttcgtatgc gcggcaacgt
     1201 gagcgacgat gcctttgaag gcgatgttcc cgccggtgtg atcgccgtgg tggtgcgtgt
     1261 tgatcagcat cgcgaagggt ttgtcggatt gctttttcag ctccgcgata agatggccgg
     1321 cctgttcggg gaattcggcg tctaccacga cgatgccttc gggggaaagg taatatgcga
     1381 tggtgccgcc tttctcatag aagatgccga cgttgtttcg caatatgcgc attttccatg
     1441 ggtcctggtg gaacaatgct tttccgggca gagagagtgc tccgagcgcg agcgaagatt
     1501 gttggaggaa aatgcggcgg tgcatggcaa gtggtttttg caaatatatt aatattccat
     1561 cttccgcagg cttggggcct tgaaccaggt gacgacgacc acggccaggg tcatgcatcc
     1621 gccgaagacg acggaaggta tcacgcccat cagacgtgcg ccgaccccgc tttcgaactg
     1681 gccgagctcg ttgctggaat tgatgaacat cgaatttacc gccgagacgc gaccgcgcat
     1741 ggggtcgggg gtctttacct ggacgatggt cccgcggatg atgacgctga tgccgtcgag
     1801 catgccgctg cagatcaggg ccacgtatga aaccaggaaa atatgggaga ctccgaatac
     1861 tatgatacaa aggccgaagc cggcgacggc gtacaggagg acataaccct ggcggcggtg
     1921 taacggccgc agtgtgagag cgatcatcat gacgatggaa ccgatatccg aagcagcgtt
     1981 gagccagccg aaaccgatgg gacccacctt caggatatcg cgcgcatata cggggatcat
     2041 ggcgaccgcg ccgccgaaaa ggacggcgaa aagatcgagg gccagcgcgc ccagcacttc
     2101 ctttgtatgg aagacgaaac gcaatccttc tttgatgctt tcccaggtgc gccgatcgcc
     2161 actggccatg atcggcttgg gacctacgga aaaaagaaag gcccacgcta tgaggacata
     2221 gacgccgacc acgctgaaac agaaaaggta actggaatgc gcaatgagga acccggccgt
     2281 ggcatgaccc gtcacggaag cgaagaggaa ggcggtcgtg ttgagggtga cggctgccgg
     2341 caatttttct ttgggaacga tctgggcgat gatggcagac agggcggggc cggcgaaagc
     2401 gcggatgacc cctgtgcaga aaatgatggt ataggtcagc cattggatcc aatgactttg
     2461 cagcgagcgg gtcagcggtc cgaaggacag tgccagaagg ccgatcacgc atagcaggta
     2521 ggtggtcgtc gttttgagca gcagggcgcg tttatcgctt ttgtcgatga catggcctgc
     2581 atacagcgcc aggcaaaagg cggggaggaa ttcggaaaga ccgatgagcc cgatagcgaa
     2641 cgggtttcgg gtgatctcat agatacgcca gccgacgatg gtggtcacca tccgaagggc
     2701 catcacgtaa aagaagcggg caaggatgaa attccggtat tcggggatct ttaagatttc
     2761 cagtgcagtc agtttgcgtt gctctgtcat ctgcactaag gtaaggttaa atattgctgt
     2821 ccttcacccc gatggccttc gagggcatcc aggatcaccc ggatatgccg ttcgttaccc
     2881 aggaaatcgg cgttgctgac gtcgacgaaa agggtagggg tattgtgctg gcggatgtac
     2941 tgcgtatagg tctcctggat tcggagcagg tagtcgtcgg gaatggactg ttcgtagggc
     3001 cggttgcgtt tgcggatatt ggcctggagt ttggagacgg gcgcatggag atagatgacc
     3061 agctccggct gtatgatctg tgattcgagg atctcgaaga atttctggta cagccggaat
     3121 tcctgttccg ccagtgtgac acgggcgaag aggaggcatt tggtgaacag ataatctgag
     3181 atggtcagag actggaagag gtcgcccgcg tggacaagtt ctttcagctg tttgtagcgt
     3241 tcggccatga agaacagctc gagcgggaag gcatattggc ccgggttctc atagaatttg
     3301 ggaaggaagg gattatcggc aaactgttcg aggatcagcc gggcgttgta atgtcgtgcc
     3361 agcaggtgcg cgagtgtcgt ttttcccgcg ccgatatttc cttcaatggt aataaagtga
     3421 tacttcatgc gccgcgtttt gcatctaagc gttttacttc cagggggtcg gtgcaggtct
     3481 gcagcaactg ctgtaccgac actccgagga cgggatggat atagtcggga gcgatctcgt
     3541 ccatcggctc caggacaaaa cgccggttgg cgatctccgg atgagggatg atcataccgg
     3601 gttcagccac tatcgcatcg ttaaaaaaaa gaatatctat atcgatggcg cgtgagccat
     3661 atttttcgag acgtttgcgt cccattttct cttcaatgcc gaggagcgag gtcatcaagg
     3721 tcgctgcgtc gaatgttgtt tccacctgaa gggcctggtt gaggaaccag ggttggtgca
     3781 gattgcccca gggcgctgtc tcatatagag aagaagtctt ccggatcgga cctgccattt
     3841 ccatgatctg tcgtcttgct tctgccagcc ggttttcccg gtctccttcg ttgccaccta
     3901 tcaataaata ggagatgttc atagattcct tactttcggg ggcccaaata tctttaattt
     3961 tggcacgctc agataattaa ttattgcaca tgcgaggatt cttcaaaaca tttttcgccg
     4021 ctttgctggc tttggcggtg tttaccgtac tcgccgtatt catcctgatg ggtgtcgtcg
     4081 gcggcatact ttcaccgggc aggcccgatg tcggcggaaa gggtgtactg gtcgtagatc
     4141 tggatcagtc ctacaaggaa caggtacagg ataatccgct gtcggatctc gggtccgatg
     4201 atcaatacga tgttccaggc gtctatgatg tcgtgaggct gttgaaacac gccgccggcg
     4261 attccgcgat caaaggcatc tacctcaaat gcaatggcga ccccaatggc tttgccacga
     4321 atgaagagat caggaaggca ttggtcggct tccgcaagag cgggaagttc gtctatgcat
     4381 atggggaagg catctcgcaa aaggcctatt atgtggccag catggccgac cgcatctact
     4441 gcaatcccaa gggagacctg gaatggaagg ggttttccgc cgagatgctt tatctgaagg
     4501 gcgcattgaa gaaactggag atcgatccgc agatctttta tgccggcaag ttcaagagtg
     4561 cgacggagcc gttgcgcgaa gaccatatgt cggacgccaa tcgtattcag ctgacgcagc
     4621 tgctgggtga tttttataac cgtcttctcc ggaccacggc ggagagcagg ggattggata
     4681 cggccgtcct tcgcaaatgc gccaatgagc atctgatcgt ccgtgcggcg gacgcgttgc
     4741 aataccggct ggtagacgga ttaaaatatg acgatgaggt caaggatgaa ttgcggtcca
     4801 agctgggtgt cgtcgcgcat ggctggatca atttcgttcc gttgggcaaa tatgcgaagt
     4861 ccgtcgattt caggaaagtg gggaaggaca ggatcgcctt gatctatgcg gaaggagacg
     4921 tcgtcgatgg aaaaggagag cgtgacgaga tcggttccga cacgtatcgc tggctgatcc
     4981 gcaaggcccg tttcgataag gatataaagg ccatcgttat ccgtgtcaac tccggtgggg
     5041 gaagctcgct ggcgagcgag aatatctggc gcgaggttga gttggccaaa aaagacaagc
     5101 ccgtaatcgt gagtttcggc gatgtggccg catcgggcgc ctattatctt tcctgcaatg
     5161 cggacagcat cttcgcgctg cccgggacca taacgggctc catcggagtg ttcaccatca
     5221 tccccaacct gaaatccttt tttaacaaca agctgggcat caccttcgac ggagtgaaaa
     5281 cggcaccgga agcggatttg ctttccacga cacggccgct gacgccctcc gagcgggcgc
     5341 tcgtacaaaa tatggtagac accatctatt ccaccttcaa gggcagggtg gccgatggca
     5401 ggaagatggg acttggctac gtggatagca tcgcgcaggg aagggtttgg tccggcactg
     5461 ccggctccga gttggggctc gtcgaccgga tcggtactct gcaggatgcg gtggattgcg
     5521 ccgcgcggat ggcgggtacc agcgattatc atcttgtgga atatccggaa cccaggagct
     5581 ttctcgaccg tctgctgggg acgtataaac acagtaccag catgaagacc gcggtccggg
     5641 aggagatcgg cgaagaaaat tatcgcagct ggatgaccct gaagaaacta aagagcatga
     5701 tcggcgtttc ggagacgaaa ctccccttcg atctgacgat tgaataatat tgaatagaat
     5761 cacaatccgg aatggcaacc aactatagcc agctgcgcgg catgctggcc atcgctaaag
     5821 gcagttttaa agcaatattg aggaatccat ccgccgtagc attcagcttc gggtttcccc
     5881 tggtctttat attggtattc ggttttatcg gcggcggcgg accttcggtg tcggtggcgc
     5941 tggccgaccc ccgcgattcg gctacggcca tcgttcgcgg attgctgaac agcaagatgg
     6001 tcaaattgtc cgatgaccgg gatagcgtca ccatccgaaa ggatctggaa cgaggtagga
     6061 tcgccgcggt gatatccttc gactcggcga agtcgccggc ggggttcatg cagtacacgg
     6121 tgcatacgca gacctcatca gccagcgggg ataaataccc gatcctgagg ctcgcgcttg
     6181 cacaggggtt cgagcgtgcc gtcgagggga ccatgccgaa ggaatacag
//
LOCUS       DASYSV010000372         6492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_971603, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000372 DASYSV010000000
VERSION     DASYSV010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971603"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VGQ51_11415"
     CDS             <1..590
                     /locus_tag="VGQ51_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV9037226.1"
                     /translation="KDNRDYELQFGVNHLGHFQLTGLLWTALREAGGARVVVVSSIGH
                     RHMGLHLDDINFESHPYSGMKAYGQSKTANILFMVQLDKIGQPYGIRAFAVHPGAIET
                     DVFRYMTEQELEDWKKGVKKFKTPGQGAATSVWCAISDQLDGSGGVYCENCDIANVVP
                     DDAPPGPGVRAYALDPEDADALWQLSEQVTGLHFG"
     gene            complement(610..1776)
                     /locus_tag="VGQ51_11420"
     CDS             complement(610..1776)
                     /locus_tag="VGQ51_11420"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV9037227.1"
                     /translation="MKKMLLAILMTSAAVPLTAQQPSPELIFRADGRSLTTSAIDSLV
                     GRLMYKARVTGLCLGVVENGRPAYIQAYGFRNKAANERNDTATCFYGASLSKSLFAYL
                     VMQLVDQGRIDLDRPLYTYLPKPLPEYEKYRDLAGDPRWKLLTARHCLDHTTGFPNFR
                     WFNPKGNNKLEFFFTPGTRYAYSGEGFVLLQLVVEAVSGRPLDSLGRERLFVPFGMRR
                     TGYVWQPDFETDYAVGHLENESTIPKKKRSSANAAGSLETTIADYTRFLAAAVRGEGL
                     SPKAFNEMTRMQIAIHSAHQFPSLDTATTTDNDVIALGYGLGWGLFTTPYGKVFFKEG
                     HDDGWEHFALCLPGQQKAFIVMTNSSNGESIFKQLFFDLSGITIPWEWERYIPY"
     gene            1940..4450
                     /locus_tag="VGQ51_11425"
     CDS             1940..4450
                     /locus_tag="VGQ51_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037228.1"
                     /translation="MEKEEIERAWARHLQRMEHRRRSRKPGPFHAFGNTGSMLRSYFR
                     IAWRNLTRSMTFSLINIGGLAIGMASAILILLWIGNEFSYDNFQTKKDRIYLLENRGN
                     YRGTVDVFAGVPQPLAPVLKTDYPQIEEVARINGTAPFTLTVGDRHFEGKGGMMTDPG
                     FLKIFSFPLAKGNLETALNTPRSMVITESFAKQLFGSDDPMGKVVRIDSNNFAVTGVA
                     KDPPRNTDLGFSYLIPYSYMKDAGWSNSGWKDFSATTIVLLKPGVTEQMADDRFRDIL
                     KMHADGIDNEIFLHPLAKWRLYSRFENGKIVGGGIEDVRLFGLLAAFILIIACINYMN
                     LSTAQSVRRAREVGIRKVVGALRPSIVLRFLGESILISFLSGVLGLIIVQMSIQGFNW
                     LTWDNLVVPYDDPYFWMGIAAFILVTGVIAGSYPAFYLSGFRPISVLKGTATRASMAK
                     EPGRSSLRRAYGMISVRKILVVFQFSIAIAFIICTIVIYRQINYGRDRDPGYNRDHLV
                     FAYVKGDMNKNYGLIRNDLLGSGAVTAVTRSNSPITYSWNSDDTYTWRGKNPGLRIPI
                     EEYHADNDFLETMGIRLIAGRTINTYLYPTDTMAILLNQSAVKLMGFKDPIGQIVTNK
                     QGNWTVIGIIKDFVVESPFNRLSPVIVQGPRNSFGAITFRLNPRYSTSDNMQKIQAIF
                     RRYNPDYPFIYRFVTEADNEKLEGQRRTGIQSALFGGMAILISCLGLFALAAYTAESR
                     IKEIGVRKVLGASITSIAALLSGGFLKLVAISFAIASPVAWWIMHSWLQSYPYHIAIG
                     WLVFVITALVSLGIALTAVSYQAIRAAMANPVDSLRAE"
     gene            complement(4739..>6492)
                     /locus_tag="VGQ51_11430"
     CDS             complement(4739..>6492)
                     /locus_tag="VGQ51_11430"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9037229.1"
                     /translation="QLFVKLAPATKYATVDAELTKILNANDKPDPKDHGTQISRLQPL
                     SDMHFNLDFGVFDESRQAHKPTLYYLLAVAAFLLLLACINFINLTTAQSSQRAKEIGI
                     RKTLGSQRRQLTLQFLTETFVLTLMAAILSLVLLPFLLKIFADFIPDDLHFNLLRHPE
                     VVLFLLVLILVVTVLSGIYPALVLAGFKPVLVLKNQAFAGSSKTRSAWLRKSLTVFQF
                     VIAQVFIIGTLLVSKQISYALNRDLGFKRDAILTFRTNYREESNKKPVLLDKLRAIPG
                     IAMVSVSSNPPSGNGTWTSTMTFNDGKKEVRENVEVKLADSNYFRMYGLRLVAGTSAM
                     QSDTTNAVAINETYLHILGYQDPRKIIGKTLNWNGTPIIVGVVADFFPRSLREPIKPL
                     LIANGTRDANVFNITLQPRNADGSNWPQTLTAIEQAFHSVYPNDDYDYQFVDETVKKF
                     YTAEQNISHLLIWATGLTIFISSLGLLGLVIYITNQRTKEIGIRKVIGANLTQLIMLL
                     TGDFLRLIALAIVIALPIAWWGSHKWLENFADKTPLSWWVFAVGGGALLLIALTILCI
                     RTLRAALVNPVKSLRAE"
BASE COUNT         1452 a         1711 c         1834 g         1495 t
ORIGIN      
        1 tgaaggacaa ccgggattac gagttgcagt tcggcgtgaa tcatctcgga cattttcagc
       61 tgacgggtct gttgtggacc gcccttcggg aggcaggcgg ggcgcgggta gtcgtagtgt
      121 cttccatcgg tcatagacac atgggattac acctcgatga catcaatttc gaaagccatc
      181 cctacagcgg gatgaaggcg tatggacaat ccaagacggc caatatcctc ttcatggtgc
      241 agctggataa gatcgggcag ccctatggca taagggcatt tgcagtgcat cccggcgcca
      301 tcgagacgga tgtcttccgc tatatgacgg agcaagaact ggaagactgg aaaaaagggg
      361 tgaaaaaatt taagacacca gggcagggcg ccgcgacatc cgtatggtgc gcgatcagcg
      421 accagctgga cggcagtggt ggggtctact gcgaaaactg tgatatcgcg aatgtggtgc
      481 cggatgatgc gcccccgggt ccgggggtgc gcgcctatgc cctggatccc gaagatgcag
      541 atgccttatg gcagctgagc gagcaggtca ccggcttgca tttcggctga accattgctg
      601 ggaagccact caatagggaa tatagcgctc ccactcccat gggatcgtga tgcccgacag
      661 gtcgaagaac aattgtttga atatactttc gccattgctg ctgttggtca tcacgataaa
      721 agccttttgc tgacccggaa gacatagcgc aaagtgttcc catccgtcat cgtgtccttc
      781 tttaaaaaag accttgccat agggggtggt gaagagaccc cagcccagcc cgtatcccag
      841 tgctatgaca tcattgtccg tcgtagtcgc ggtgtccagc gaaggaaact gatgcgccga
      901 atggatcgcg atctgcatcc gggtcatttc attaaaggcc ttcggcgaca acccttcgcc
      961 gcgaactgcg gccgccagga aacgggtata gtccgcgatc gtcgtctcca gggatccggc
     1021 agcattcgcg ctgctgcgtt ttttcttcgg gatcgtcgac tcattttcga gatgacctac
     1081 ggcataatct gtttcgaagt cgggctgcca cacatatccg gtccgccgca ttccgaacgg
     1141 aacgaataat cgctcccttc ccagcgaatc caatggtctt ccggacaccg cctccaccac
     1201 cagctgcaac agcacgaacc cttccccgga atatgcgtac cgcgttcccg gcgtaaagaa
     1261 aaattccagc ttattgtttc ccttcggatt gaaccaccgg aaattcggga agcctgtagt
     1321 atggtcgaga cagtgccgtg ccgtgagcag cttccacctg ggatcacccg cgagatcgcg
     1381 atatttctca tattccggaa gaggcttcgg caaataggta tacaagggcc ggtccagatc
     1441 gatcctgccc tgatcgacca gctgcatcac tagataggcg aagagcgact tggacaggga
     1501 ggcgccgtaa aaacaggtgg ccgtatcgtt gcgttcattt gccgccttgt tcctgaaccc
     1561 gtaggcctgt atgtaggcgg gtcggccatt ctccacgacc ccgagacata atcccgtcac
     1621 ccgggcttta tacattagcc taccgacgag gctatcgatg gcggaagtcg tcagagaccg
     1681 gccatcggcg cgaaatatca attccggcga aggctgctgc gcggtcagtg ggacagcagc
     1741 cgatgtcatc aggatagcaa gaagcatctt tttcatgaat gtagtcgttc ggctatttaa
     1801 agatagggat ttgatctgtc gcataagcga ataccctgcg tatcgaaagc ggacactttc
     1861 cggaaacgag ccgatcatag attattgtta ttcaatattc taattttcag gtattgcctt
     1921 tgccgcgaaa tcttccgata tggaaaaaga agagatagag cgtgcctggg cccggcatct
     1981 tcaacgcatg gaacacagaa gacgctcccg gaaacctggc cccttccatg cttttggcaa
     2041 caccggcagc atgctccgca gctatttcag gattgcctgg cgcaacctta ccaggtccat
     2101 gaccttttct ctgatcaata tcggcggtct cgccatcggg atggcaagcg ccattctcat
     2161 cctgctctgg atcggcaacg agttcagcta tgacaatttc caaacgaaaa aggatcgcat
     2221 ctatctcctc gaaaatcggg gaaactatcg gggcacggta gacgtctttg ccggagtgcc
     2281 ccaaccgctg gcgcctgtac taaagaccga ttacccgcag atcgaagagg tagcccgcat
     2341 taatggcact gcaccattta ctcttaccgt aggcgatcgg catttcgaag gaaagggagg
     2401 gatgatgacc gatcccggct ttttaaagat attcagtttc ccgctggcaa aagggaacct
     2461 ggagacggcc ctgaatacgc cccgctcgat ggtgattacg gaatccttcg caaaacaact
     2521 attcggcagc gatgatccga tgggtaaagt ggtccgcatc gacagcaata attttgccgt
     2581 caccggcgtc gcaaaagacc cgccccgcaa tacggatctg ggttttagtt acctgatccc
     2641 ctatagctat atgaaggatg cgggatggtc caactccggt tggaaagact tttcggcgac
     2701 gactatcgtg ctgctcaaac ccggcgtcac cgaacaaatg gccgacgatc gattccggga
     2761 tatcctgaag atgcatgccg acggcatcga taatgagatc tttttgcatc cgctggccaa
     2821 gtggcggctt tactcccgtt ttgaaaatgg gaagatcgtg ggcgggggta tcgaggatgt
     2881 gcgtttgttc ggtcttctcg ccgcattcat cctgatcatc gcctgtatca actatatgaa
     2941 cctgagcacg gcacaaagcg tccggagggc gagagaggtg gggatccgca aggtggtggg
     3001 agccctgagg ccctcgatcg tgctgcgatt cctgggcgag tcgatcctga tctcttttct
     3061 ttcgggtgtg ctgggtctga tcatcgtcca gatgagtatc cagggtttca actggcttac
     3121 ctgggacaac ctcgtcgttc cctatgacga tccgtatttt tggatgggca ttgcggcgtt
     3181 tatccttgta acgggcgtga tcgcggggag ctatcctgca ttttatcttt ccggtttccg
     3241 gccgatcagc gtcttaaagg gaaccgctac gagggcgagc atggcgaaag agcccggccg
     3301 aagttcgctc aggagggcct atggcatgat cagcgtccgg aagatactgg tagtatttca
     3361 attcagcatt gcgatcgcct tcatcatctg cacgatcgtc atctaccggc agatcaacta
     3421 tggccgtgat cgcgacccgg ggtacaacag ggatcacctg gtattcgcgt atgtgaaagg
     3481 cgatatgaac aaaaactacg ggcttatccg aaacgatctg ctgggcagcg gggctgtgac
     3541 ggccgtcacc cgaagcaatt ctccgatcac ctatagctgg aactcagatg atacctacac
     3601 ctggcgcggt aagaacccgg gtctgaggat ccctatcgaa gaatatcacg ccgacaacga
     3661 ctttttggag acgatgggta tccggctgat cgccggccgc accatcaaca cctatttata
     3721 tccaaccgac acaatggcga tcctgttgaa ccagtcggcc gtaaagctca tgggcttcaa
     3781 ggacccgatc ggccagatcg tgacgaacaa acaggggaat tggacggtga tcggtattat
     3841 aaaggatttt gtggtcgaat cgccctttaa tcgcctgtcg cccgtcatcg tccagggacc
     3901 caggaactca tttggcgcca tcacttttcg gctgaatccc cgctattcta cgtccgacaa
     3961 catgcaaaag atccaggcta tctttcgaag atacaacccc gactacccct tcatctaccg
     4021 atttgtcacc gaagccgaca atgaaaagct cgaaggtcag cggcgaacgg gtatccagtc
     4081 ggctctcttc ggcggcatgg cgatcctgat ctcctgtctt ggactttttg ccctggcggc
     4141 ctatacggcc gaaagccgca taaaagagat tggcgtgcgg aaagtgctcg gcgcatcgat
     4201 aaccagcatc gccgccctcc tgtcgggagg tttcctgaaa ttggttgcaa tttcctttgc
     4261 catagcctct ccggtcgcct ggtggatcat gcattcctgg ctgcaaagct atccctacca
     4321 tattgccatc ggatggctgg tattcgtgat caccgcgctg gtctcgttgg gcatcgcact
     4381 gacggccgtc agctaccagg cgattcgcgc cgctatggcc aatccggtgg acagtctgcg
     4441 agctgaatga tccgctgcga atcgccatcg ccaccgccaa tccgataaac gctctgcgaa
     4501 acgattaaac cggcggcgcg gcttccggtc ttacgacaga ataattaaat tacaccatgc
     4561 ttttgggatt catttcgccg atgctcaacc tggtgatcct gctgggcatg ctgcagggat
     4621 tcatcttcag catcctgctg ttcggttccg tccgccgccg gcccgcccgc tgatattaaa
     4681 gtggacacga ccgactatct cgtcgccgtc gaccgctgag acggcgaaag agatctgttc
     4741 actccgccct gaggcttttg accggattga caagggccgc ccgcagggtg cgaatacaga
     4801 ggatggtcag cgcgataagc aggagtgcgc caccgcctac cgcgaagacc caccagctga
     4861 gcggcgtttt gtcggcaaag ttctcgagcc atttgtgact gccccaccat gcgatgggga
     4921 gggcgatgac gatcgcaagc gcgatcagcc ttaggaaatc gccggtgagc agcatgatca
     4981 gctgggtgag attcgcgccg atgaccttgc ggatgccgat ctcttttgtc cgctgattgg
     5041 tgatatagat gacaaggccg agaagcccga ggctactgat gaagatggtc agaccggtgg
     5101 cccaaatcag gagatgagaa atgttctgtt cggcggtata gaattttttg acggtctcat
     5161 cgacgaactg gtagtcatag tcgtcgttcg ggtacaccga atggaaggcc tgttcgatgg
     5221 cggtgagggt ttgcggccag ttggagccgt ctgcattgcg cggttggagc gtaatattga
     5281 acacgttggc atctcttgta ccgttggcga tgagcagtgg tttgatcggt tcccggagcg
     5341 accgcgggaa gaagtcggcg acgacaccta ctatgatggg agtgccattc cagttgagcg
     5401 tttttccgat gatcttccgg ggatcctggt acccgaggat atgcagatag gtttcattga
     5461 tcgccaccgc gttggtggta tcgctctgca tggcgctggt tcctgccacc agtcgtaatc
     5521 catacatgcg gaaatagttg ctgtccgcga gttttacctc tacattttcc cggacctcct
     5581 ttttcccgtc gttgaacgtc atggtactcg tccatgtccc gttgccggat ggcgggttgc
     5641 tggaaacact gaccattgct ataccgggaa tggctcttaa tttatccagc aggaccggct
     5701 tcttgttgga ttcctcccgg tagttggtac ggaaagtgag gatcgcatcc cgtttaaaac
     5761 cgagatccct gttcagggcg tagctgatct gtttggagac cagcagcgtg ccgatgatga
     5821 agacctgcgc gatcacgaat tggaatacgg tgagcgattt gcgcagccac gccgagcggg
     5881 tcttgctgct gcccgcaaag gcctggttct tgagcacaag gactggtttg aaacccgcca
     5941 gcacgagtgc gggatagatc cccgagagga ccgtaacgac gaggatcagg acgagaagaa
     6001 agagcacgac ctccggatgc cgcagcaagt tgaagtgcag gtcgtcgggt atgaagtcgg
     6061 cgaatatctt taacaggaaa ggtagcagaa cgagggagag gatggccgcc atgagtgtca
     6121 atacaaacgt ttcggtgagg aattgtaagg tcaattggcg tcgttggctg cccaatgtct
     6181 ttcggatgcc tatttctttt gcgcgttgcg aagactgggc ggtggtgagg ttgatgaaat
     6241 tgatgcaggc cagcagcagg agaaaggcgg cgacggcaag caggtaatag agcgtgggtt
     6301 tgtgcgcctg ccggctttca tcgaaaaccc cgaaatcgag attgaaatgc atatcgctca
     6361 ggggttgcag cctcgatatc tgcgtgccgt ggtccttcgg atctggtttg tcgtttgcat
     6421 tgagtatttt ggtgagttcg gcgtcgacgg tcgcgtactt cgttgccggc gccagtttga
     6481 cgaagagttg at
//
LOCUS       DASYSV010000373         6941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2505127, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000373 DASYSV010000000
VERSION     DASYSV010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2505127"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VGQ51_11435"
     CDS             <1..591
                     /locus_tag="VGQ51_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037230.1"
                     /translation="LCDVYGFSLRNLAPAATQPSGSQHPSGPAGTVLQYNPALAVTFV
                     ENHDIVRDNPIVNDKMLAYAYILTHEGYPCVFWQDYYNWDLAQPTNQSGIDALVKAHG
                     SNAGGPTEVLYVGDNLYIMQRTGTNAQKGLVFVLNNSAATSGAQVHTHWPNTTFHPVA
                     WRGHDNTDIPAPRTTAPDGTAEFSAPPRGYTVYLPT"
     gene            640..3087
                     /locus_tag="VGQ51_11440"
     CDS             640..3087
                     /locus_tag="VGQ51_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9037231.1"
                     /translation="MLKNYFLVALRNFRRNKLFSIINIVGLAIGISASLIITALVWYD
                     FSFDRFEKDRDRIYRVVSEWNFQGNISHWGSTCVPIGPAIEKELTGIELVVPFRTADR
                     LKVSIPWPDASRPLVLKKQKDLIYADSRYFNLLHYDWLAGDLKTSLRQPYQVVITASN
                     AHLYFPHLPYSDIIGKPIFFDDTIQATITGIVKDISYNTDFNFRGFISYSTMETTRMK
                     PQNWDNWGSSNSADQLFVRLSPGASATKLVAAINRLREDHEHADATRKSKTTTLLQPF
                     KDMHFDSGYGIFDESRQPHKSVLYGLLAVAAFLLLLACINFINLTTAQASQRAREIGI
                     RKTMGGHRRQLAFQFLNETFLLTVLATLLSLAITPLLLKVFKDFIPEDFHFNPFTQPI
                     VFVFLGVLILAVSLLSGIYPALVLSSYNPVLVLKNQAYANTGRTRTAWLRKTLTVSQF
                     VIAQVFIIATLLVDRQINYSLNKDMGFKKDAVLYFSTNPRVSAQMKVVLLNKLRAIPG
                     VQRAGLSSDPPSSGGSWSSTITYMDGKKEIQDNVQIKVADSNYFPIYKFHLVAGTSAP
                     QSDTMNAVVINETYARRLGFLDPQKAIGKQFKWQGNPYIVGVVADFHQHSLRDPIKPL
                     LIANGGRFLNTVNVALEPQNGDGTAWPHTVASIEKAYQSVYPGEDFDYQFLDASIAKF
                     YNAEKNTSRLLMWSTGLTIFISCLGLLGLVIYVTNQRTKEIGIRKVIGASVSQIIVLL
                     SRDFMKLIALAILIAFPIAWWGSHKWLENYAYRTNISWWIFAVGGGLLLAIALIILCL
                     RTLRAAVVNPVESLRSE"
     gene            complement(3264..4181)
                     /locus_tag="VGQ51_11445"
     CDS             complement(3264..4181)
                     /locus_tag="VGQ51_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037232.1"
                     /translation="MQYPFFLLLAGFFSSSLYGQNFLIREPDSAGNFEFQNVCKGKVT
                     VTSGSYEVLKGQPVKFMTKWGRSKDGKFFWTNGENIYQFHFNSQKTEIISSNWHFIVE
                     FYVCDSAAYIVYNPSRQEGEHDNRYSPGLRFCCLNLNNGHRTNYQFPNNINFTNLSIS
                     PDRMWAVFVNTIFTTDENVKKYQLVLYDLKAGSVQIIDSGITSKDQWFGNDDKYNSCF
                     WPSNTVLIYYKHMRKGDNGSILSYNILSKKIEPAINSIPERDFTWFGYFHHAFFFSNR
                     TSIYKTVNGRDKEIIVGNTKNRVLEAVLY"
     gene            complement(4455..6080)
                     /locus_tag="VGQ51_11450"
     CDS             complement(4455..6080)
                     /locus_tag="VGQ51_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037233.1"
                     /translation="MDKTEAGRIIIAIHGVGDTKKGEVAKGLAEGLGIPKASMDTLLV
                     DGNDYQRLLTDDGKLEIVEVNWSDILKPDKGWWSILKQAGLIITSMLSLAEQRWQMRF
                     RAKLNVFRLLRFCLEALTPGAVFFSLSTVLGMVVKRPGFVVGLLLLVIAFFAWFTYKA
                     TAYSKNYRWGWVWLAPLTAYLASVWTNKYTGQLQELSMEIREYGQVITSGLAFALFLA
                     YLYLYVRKKDKDCIATEFSFTYLPFLAFNSAATMIAFLSLRALKEFGDENMLLAWDKA
                     AKNFLRFDLAKAELMTTIIYSIIGIIPLIYTVLYMKKSGYPGGIKKAGLINKKGRLLQ
                     QALALLLRVSPLLLLFLTGYLTYLVITWNKDVSHIEPIWHIYEISILRTIPFFSWFVG
                     PLAIVTGVVGDILFYIQPDENARHGTREKCLYRLKCAMEYAHSKPGAEKTVVAHSWGT
                     VVAVDYLCKYPTPVRLVTLGSPLEALCYQFLGMEVMNPALLSGWLNAYRYGDYIAGPL
                     APSSKAINELIGDGGHMDYWKDKIMGEAILPDE"
     gene            complement(6241..>6941)
                     /locus_tag="VGQ51_11455"
     CDS             complement(6241..>6941)
                     /locus_tag="VGQ51_11455"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEV9037234.1"
                     /translation="ATSAMDETSKAFTVTPYGLTEKGKAIVRRMNELGMLVDLSHVGE
                     KTFWDAIHTTTKPIICSHSSVWNLCPVFRNLKDDQIKAVGANGGVIQVNFYSGFLDSN
                     YQPRVTRFLHRHKTELDSMNAAHAPQYAINEYLSKKYPAEADSLRAPMSLVLDHIDYI
                     VRLIGPDHVGLGSDFDGIESPPQQLDDVSTYPLITRELLKRGYKPDDIKKILGGNFIR
                     VLKANEVGKAVAVK"
BASE COUNT         1663 a         2075 c         1490 g         1713 t
ORIGIN      
        1 ctctgtgatg tctacggatt cagcctgcgc aacctcgccc ccgccgccac gcaaccctcc
       61 ggctcacaac acccttccgg cccggccggc accgttctcc agtataaccc cgccctcgcc
      121 gtcaccttcg tcgaaaatca cgacatcgtc cgcgacaacc ccatcgtcaa cgacaagatg
      181 ctcgcctatg cctatatcct cacgcacgaa ggctatccct gtgtcttctg gcaggactac
      241 tacaactggg atctcgccca accaaccaat caatccggta tcgacgccct cgtcaaagcc
      301 catggatcca atgccggcgg ccccacggaa gtcctgtacg tcggcgacaa tctctatatc
      361 atgcaacgga ccggcacaaa cgcccaaaaa ggtctggtct tcgttctcaa caattccgcc
      421 gccaccagcg gcgcccaagt ccacacccac tggcccaata ccacctttca tcccgtcgcc
      481 tggcgtgggc atgacaatac ggacattccc gcaccccgaa ccacggcccc cgatggtacg
      541 gctgagttct ccgccccgcc ccgcggctat acggtatatt tacccacata atctccgaaa
      601 ctcttcgtat atttaatcta acggcaaacc catcgccata tgctcaaaaa ttatttcctc
      661 gtcgcccttc ggaatttccg gcgcaacaag ctcttctcga tcatcaatat tgtcggtctc
      721 gccatcggta tcagcgcctc gctgatcatc acggcgctgg tttggtacga cttctccttc
      781 gacagattcg agaaggaccg cgaccgtatc taccgcgttg tctccgagtg gaatttccag
      841 gggaacatct cccattgggg tagcacctgt gtaccgatag ggccagccat cgaaaaagag
      901 ctcaccggca tcgagctcgt cgtgcccttc cgtaccgccg accggctgaa agtgtccatt
      961 ccctggcctg atgccagccg cccgctcgtc ctcaaaaaac aaaaggatct catctatgcc
     1021 gacagccggt atttcaatct tcttcattac gactggctgg ccggtgacct gaagacttct
     1081 cttcgtcagc cctaccaggt ggtaatcacg gccagtaacg cgcatctcta tttcccgcat
     1141 ctcccgtaca gcgacatcat cggcaaaccc atctttttcg acgatacgat acaggccacc
     1201 attaccggca tcgtcaaaga catttcttat aatacagatt tcaatttccg cggattcata
     1261 tcgtattcta cgatggagac cacccgcatg aagccgcaga actgggacaa ctggggcagt
     1321 tccaattctg ccgaccagct ctttgtccgt ctgtcgcccg gtgcaagcgc tacaaaacta
     1381 gtcgcagcca tcaaccgtct ccgggaagat catgagcatg ccgacgccac caggaaaagc
     1441 aagaccacca cgctcctgca gcctttcaag gatatgcatt tcgactccgg gtacggcatc
     1501 ttcgacgaga gccgccagcc tcacaaatcc gttctctacg gactcctggc cgtagctgca
     1561 ttcctcttgc tgctcgcctg tatcaatttc atcaacctga cgaccgctca ggcttcccag
     1621 cgtgcgcgcg agatcggcat tcgcaagacg atgggaggac accgtcgtca gctggccttc
     1681 caattcctga acgaaacctt cctgctgacc gtactcgcca cgctgctatc gcttgcgatc
     1741 acgccgctcc tgctgaaggt gttcaaggat tttattccgg aagattttca ttttaatcct
     1801 ttcacgcagc cgatcgtatt cgttttcctg ggggtgctga tccttgccgt cagccttctc
     1861 tcgggcatct atcccgcgct cgtgctgtct tcttacaatc ccgtgctcgt tctcaagaac
     1921 caggcctatg ccaataccgg caggacccgc actgcctggt tgcgcaagac ccttaccgtg
     1981 tcgcagttcg tcattgccca ggtcttcatc attgccaccc tccttgtgga tcggcagata
     2041 aactactcgt tgaacaagga catgggcttt aaaaaagatg ccgtcttata cttctctacc
     2101 aatccccgcg tcagcgcaca aatgaaggtc gtccttctta acaaactccg cgctatcccc
     2161 ggtgttcagc gcgccggtct cagctccgac ccaccctcca gtggcggctc ctggagcagc
     2221 accatcactt atatggatgg caaaaaggag atacaggaca atgtccagat caaagtagcc
     2281 gactcgaatt atttcccgat ctataaattt catctggtcg ccggcacgtc cgctccccaa
     2341 agcgatacca tgaatgccgt cgtcatcaac gagacctatg caagacgcct tggcttcctc
     2401 gaccctcaga aagccatcgg caaacagttc aaatggcagg gcaatcccta catcgtcggg
     2461 gtcgtcgccg atttccatca gcattccctc cgcgatccga tcaagccgct gctcatcgcc
     2521 aacggcggcc gtttcctgaa tacggtgaat gtcgccctcg agccccagaa tggggatggc
     2581 accgcctggc cgcacaccgt cgcctcgatc gagaaggcct accagtcggt ctatcccggc
     2641 gaggatttcg attaccagtt cctcgacgcg tccatcgcca aattctacaa tgcggaaaag
     2701 aacacctcgc gtctgctgat gtggtccacc ggtctcacca tcttcatcag ttgtctcggt
     2761 ttgctcggcc tggtgatcta cgtcactaac cagcgtacca aagagatcgg tatccgcaag
     2821 gtcatcggcg cctccgtctc gcagatcatc gtcctcctct cgcgcgactt catgaagctg
     2881 atcgccctcg cgatcctcat cgccttcccc atcgcctggt ggggcagcca caaatggttg
     2941 gagaattatg cctatcgtac caacatcagc tggtggatct tcgcggtcgg cggcggcctc
     3001 cttctcgcga tcgcgctcat catcctctgt cttcgcaccc tccgcgcggc cgtcgtcaat
     3061 cccgtggagt ccctgcgcag cgagtagcgc aggggcaggg ggccagcatt tgcgcttcga
     3121 ttaaacatcg tagagcttct tctttgccac cggcaaaggc tcgctcgtaa ccttcgcttt
     3181 gctcggttac acttcgttca acggcggaat tgacttcgcc ctacctcgat acttgcacct
     3241 gcgtcagcta ttggaatcgc tagctagtat agtacagcct ccaataccct gttttttgta
     3301 ttgcccacga tgatctcttt atctcttcca ttcactgttt tatatatact agtccgattt
     3361 gaaaagaaaa atgcatgatg aaaatagccg aaccaggtga aatctctctc cggaatcgaa
     3421 tttatggccg gctcaatctt cttcgacagt atgttgtacg ataaaatact gccattgtcg
     3481 cctttacgca tatgtttata atagattagg acggtattac ttggccagaa gcaagaatta
     3541 tatttatcat cgtttccgaa ccactggtct ttgctagtga tgcctgagtc gataatttgc
     3601 acgcttccag ctttcaaatc atataacaca agctgatatt ttttaacatt ttcatccgtg
     3661 gtaaaaattg tgttgacaaa taccgcccac atccgatccg gtgaaataga taagtttgtg
     3721 aaatttatgt tattggggaa ttgataattg gttcgatgac cgttgttcaa atttagacaa
     3781 caaaatctca agccgggcga ataacgattg tcatgctcgc cctcttgcct tgaaggatta
     3841 taaactatat aggcagcgct atcacaaaca tagaattcga ctataaaatg ccagttagaa
     3901 gaaatgattt cggttttttg ggaattgaaa tgaaattgat aaatattttc cccattcgtc
     3961 cagaagaatt tcccgtcttt agatctgccc catttcgtca taaacttcac cggctggccc
     4021 ttcaaaacct catatgaccc ggatgtaacc gttaccttcc ctttgcaaac gttttggaat
     4081 tcaaagttcc cggcagaatc aggctcccta attagaaagt tctgcccata aagagatgac
     4141 gaaaaaaaac cggctaataa aagaaaaaat ggatattgca tgcattaaat gttgacgtgg
     4201 ttactctatt catatttgtg aggccttaat atgtattgcc ccgagacctc ttcccaatgg
     4261 aaaagcctgc ccgcgcggtt ttactattcg ttggtctaca cctccgtact attcttctcg
     4321 ctcagcgtca aattggaaaa tctcaaatat caccgctgat atggcgtgct ctatgttttt
     4381 ctcatctatc tgctgggcat cgtctgtctc gcctatactg ccaactttat acttcaaaag
     4441 tagtgatagc accgtcactc atccggcagg atcgcttcgc ccattatctt atccttccaa
     4501 tagtccatgt gtccaccgtc tccgatcaac tcgttgatcg ccttcgatga aggagccaaa
     4561 ggtccggcga tatagtcccc ataacgatat gcatttagcc aaccggagag tagcgccggg
     4621 ttcatcacct ccattcccag gaactggtaa cacaaagcct cgagcgggct tcccaacgtt
     4681 accagccgga cgggtgtagg atacttacat agataatcga ccgccacgac tgtcccccaa
     4741 ctgtgtgcca cgactgtctt ttccgcccct ggtttagaat gggcatactc catcgcgcat
     4801 ttaagacgat aaaggcattt ttctctggta ccatgcctcg cgttctcgtc tggctgtata
     4861 tagaaaagga tatccccgac tacccccgta acgatggcca gcgggccgac gaaccaggaa
     4921 aaaaacggga tagtcctaag aatggatatt tcgtatatat gccagatggg ttcaatatgg
     4981 ctgacatctt tgttccatgt gatcaccaga taggtaagat agcctgtcaa aaaaagcaaa
     5041 aggagcggag aaacgcgtaa aagcaatgca agcgcctgtt ggagaagacg tcccttttta
     5101 tttatcagcc ctgccttctt tattcccccc gggtaaccgc tctttttcat atacagcacg
     5161 gtatagatca gcggtattat acctataatt gaatagataa tggtcgtcat caactccgcc
     5221 ttagccaaat caaaccgcag gaaattcttg gctgccttat cccaggcgag cagcatattt
     5281 tcatcgccaa attcctttag cgctctcaac gacaaaaaag cgatcatagt cgccgccgaa
     5341 ttaaacgcga gaaacggcag gtaggtgaag gagaattcgg tcgcaatgca atccttgtct
     5401 ttttttcgaa cgtacagata aaggtaagcg agaaacaagg caaacgccag tccggacgta
     5461 atgacttgcc catattcccg gatttccata gagagttcct gtagctgacc ggtatactta
     5521 ttggtccaaa cgctcgccag gtaggccgtt aaaggggcaa gccacaccca cccccaccga
     5581 tagttcttac tgtacgccgt agccttatag gtaaaccatg cgaagaacgc aatgaccagg
     5641 agcaataggc caacgacgaa cccgggcctt ttgaccacca ttccaagcac cgtcgacagc
     5701 gaaaaaaaga cggcgcccgg cgtcagcgcc tccaggcaaa atcgaagtaa gcgaaaaacg
     5761 ttcagcttcg cgcgaaaacg catttgccat cgctgctccg ccagcgaaag catagacgtt
     5821 atgatcagac ctgcctgttt taaaattgac caccaacctt tgtccggctt caggatatcc
     5881 gaccaattca cctcaacgat ctccagtttt ccatcgtccg ttaaaagccg ttgataatcg
     5941 ttgccgtcaa ccagcagcgt atccatagaa gctttcggga tgccgaggcc ttccgcaagg
     6001 cccttggcca cctcgccttt tttcgtgtcg cctaccccgt gtatagcgat gataattcgc
     6061 ccggcttctg tcttgtccat agcggcaaag aaaaggttaa atgcaggtct atgacaccgt
     6121 gaaaacacct ttttttccaa ctcgccttgc caaacatctc cgatcaagca cactgcgctc
     6181 cttcattcgt agacaccaga tttacttagc aaatccaaca accattccac gattaccccc
     6241 ttatttcacc gccaccgcct tccccacctc attcgccttc agcacccgga taaaattacc
     6301 tccgagtatt ttcttaatat catccggctt ataccccctc ttcaacaatt cccgcgttat
     6361 cagcggatat gtcgagacgt catccaactg ttgcggcggc gactcgatcc cgtcaaagtc
     6421 cgagcctaac cccacgtggt ccggcccgat cagccttacg atataatcta tatggtcgag
     6481 cacgagcgac attggcgccc tcagcgaatc tgcctcagcc gggtattttt tggacaggta
     6541 ttcattgatc gcatattgtg gcgcatgtgc cgcgttcata ctgtccagtt ccgtcttatg
     6601 gcgatgcaaa aacctcgtca cgcgcggctg ataattgctg tctaggaagc cggaatagaa
     6661 attcacctgt atgaccccac cattcgcccc gaccgccttg atctgatcgt ctttcagatt
     6721 gcggaagaca gggcataggt tccacacgct gctatgactg cagatgatcg gtttggtcgt
     6781 cgtatggatc gcatcccaga acgtcttttc gccgacatgt gatagatcga ccagcatacc
     6841 cagctcattc atccggcgga cgatcgcctt tcccttttcc gtcaggccat agggagtcac
     6901 ggtaaaggcc ttcgaagtct cgtccattgc cgaggttgcc c
//
LOCUS       DASYSV010000374         6570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1225804, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000374 DASYSV010000000
VERSION     DASYSV010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225804"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /gene="typA"
                     /locus_tag="VGQ51_11460"
     CDS             <1..1687
                     /gene="typA"
                     /locus_tag="VGQ51_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV9037235.1"
                     /translation="DLEKERGITIFSKNAAVEYNGTKINVIDTPGHSDFGGEVERVLK
                     MADGVLLLVDAFEGPMPQTRFVLQKALQLDLKPIVVINKVDKPNCRPDEVHDAVFELF
                     FNLDATEEQLDFPTFYGSGKNGWFNSKNEPCEDITPLLDGILQYVPEPRVAEGPLQMQ
                     ITSLDYSSFLGRIAVGKVARGSIKENQPVALMQTDGTIRRSRVKELYTFMGMGKKKAT
                     EVMAGDICAVVGLEDFNIGDTIADAENPEALPIISVDEPTMSMLFGINNSPFYGKDGK
                     FVTSRHLRDRLLKETEKNLALRVEDTDGADSFLVYGRGILHLGILIETMRREGFELTV
                     GQPQVLVKEINGKKHEPYENLVVDVPGEFSGKVIDLVTQRKGELLVMETKGEMQHLEF
                     EIPSRGLIGLRSTMLTGTAGEAVMAHRFLEYKPWKGPIPGRNNGVLLSKNQEKTTAYS
                     IDKLQDRGTFFVDPGEDVYSGQIIAEHIKPGDLIVNATEGKKLTNMRASGSDDAVRIA
                     PKKQMTLEECMEYIQQDECIEVTPKKIRLRKTILDEEERKKYQRMLKTEEVGG"
     gene            1710..1949
                     /locus_tag="VGQ51_11465"
     CDS             1710..1949
                     /locus_tag="VGQ51_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037236.1"
                     /translation="MSKYLVIFLFFALAMGIQPATAQCSICTRTAQQLGERPAKALNK
                     GIIYLAFTPLAIFFVIGYRWARSEGHGWWKSEDHD"
     gene            1942..2877
                     /locus_tag="VGQ51_11470"
     CDS             1942..2877
                     /locus_tag="VGQ51_11470"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9037237.1"
                     /translation="MTKPHLVSIVLGTYNGETFLEEQLRSLLVQTYPHLEIVAIDDGS
                     TDGTVGILKDFAARDPRIKIILNEKNLGFIRNFEKGCTLSSGKWISLCDQDDYWYPDK
                     VERMVNAIGDLPMIYCDSELCDQQLQPNGKRISDLVRYQSFYDCRQLCVFSRMYGHAT
                     LITRDLFDRCRPFLEGIPHDGWLAFHATLYGGVAYLPEVLVKYRQHAANVYGVVGRRQ
                     KKKNRPARSERRRKELARIRLRMDAFYKACPGDLAPQKELLRRLVRCYRNFSPLNDIR
                     RVGLFFANYKLLLAVKNYSTWRKYLFCLKMLVKIK"
     gene            complement(2870..3781)
                     /gene="meaB"
                     /locus_tag="VGQ51_11475"
     CDS             complement(2870..3781)
                     /gene="meaB"
                     /locus_tag="VGQ51_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEV9037238.1"
                     /translation="MAEREDQLWTALRRGDRRALARAISYIENGVNDFAGLLATCPPA
                     NIPVIGITGPPGAGKSTLVDALIGYYVAARQRVAVLCIDPSSSLYYGALLGDRIRMSD
                     WYNHPDVYIRSLATRGSPGGLHPRAIEITNLLKHAPFDHLLIETVGVGQNEIAVAGLA
                     DTTIVVLAPEAGDDIQSMKAGLLEVADIFVVNKSDRPDADLFVRNLRSMQSPAFRREG
                     RDIPVVKTIASQKQGIGELADHIGEHLKKNVASQRRYHLLAEQAWLLIQQQRMQDIDR
                     QTLAEKIETLSKNGEFNLFRFVREWIT"
     gene            3794..4852
                     /locus_tag="VGQ51_11480"
     CDS             3794..4852
                     /locus_tag="VGQ51_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV9037239.1"
                     /translation="MSTEVTIFTGMATTFIISRTDAIGDVVLTLPVAGVLRSLYPQSH
                     IYFLGRSYTEDVVKACEHVDGFLNWDEWKTLPDADAARALADTKADTIIHVFPDRRIA
                     RLARQAGIRQRIGTTNRLYHWLTCNRLIRLSRRHSPFHEAQLNLQLLAPVGAEELYPL
                     ASIAGFYGLTRLPELPSSIASLPASGKFNLVLHPKSRGSAREWGLDNFRELIRLLPQD
                     RFRIFITGTAAEGQLVQPLLQEFPLVTDLTGRLSLGQLMTFLSVTDGLIAASTGPLHL
                     AAALGIHAIGIYPPIRPMHPGRWAPVGRRAKVFVREAECEACRKTADCVCMRGIRPQQ
                     VGDYLLTLTSPVRRGRSL"
     gene            4872..5516
                     /locus_tag="VGQ51_11485"
     CDS             4872..5516
                     /locus_tag="VGQ51_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV9037240.1"
                     /translation="MTNFIPVFPLGIVVYPGEMVNLHIFEPRYKQLINECYTEGKPFG
                     IPTVIDNRLSEMGTLVRVTEVVKTYDNGELDIKTLGLRVFRVLELIKNVPDKLFSGAI
                     VNYPDNIEGPVKRDLMQRVVNAIRELHRLLNIEKDFHKPDEELASYDIAHHVGLSLEQ
                     EYELLGLLREEQRQEYIKRHLGKVLPVIAEMETLKERVKLNGHFKNLSSFKFED"
     gene            5518..6243
                     /locus_tag="VGQ51_11490"
     CDS             5518..6243
                     /locus_tag="VGQ51_11490"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEV9037241.1"
                     /translation="MATTLCFDFGNTRMKCGVFTDGNFSEELVLHSDTDETIAGLLEK
                     YRPERSILSSVIHHNPGMEKLLGAGSRFHLLGHQSRLPVTTPVGKPETIGADRLALVV
                     AAVTLFPGSNNLVIGLGSAITYNYVNKYKEFIGGGISPGMEMRFRSLHEFTAKLPLVK
                     ADWNFPLAGYDTRTNILSGVILGMAKEVEGIISAYEEKYANFNVLLSGGDAVYFTRYL
                     KKKIFADPYLIYKGLYAISVFND"
     gene            6236..>6570
                     /locus_tag="VGQ51_11495"
     CDS             6236..>6570
                     /locus_tag="VGQ51_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037242.1"
                     /translation="MIKGLIGSRAIRGGAGWPLLLLIAVLAVSCGGKTAVRPSNLVHQ
                     IIPEEADSITSLLSEPVDSGSKLAARVKGKLTSPFMLRYDRTDSPYAEFPRSLHVDFY
                     NDSMQIESKL"
BASE COUNT         1533 a         1814 c         1809 g         1414 t
ORIGIN      
        1 cgatctcgaa aaggaacgcg ggatcaccat cttcagcaag aatgcggcag tagaatataa
       61 tggcaccaag atcaatgtca tagatacccc cggtcactcc gatttcggag gcgaggtaga
      121 acgtgtgttg aaaatggcgg acggcgtatt actattggtc gatgctttcg agggaccgat
      181 gccccaaacc cggttcgttt tgcagaaggc cctgcaactc gatctgaaac cgatagtcgt
      241 catcaataag gtggacaaac cgaactgtcg tccggacgag gtccatgacg ccgtcttcga
      301 actattcttc aatctcgacg ccaccgagga acagctggat tttcccacct tctacggcag
      361 cggtaagaac ggctggttca acagcaagaa cgagccctgc gaggatatca ccccactcct
      421 ggacggcatc ctgcaatacg ttcccgaacc cagggtcgcc gaaggccctc tgcagatgca
      481 gataacctcg ctggattatt cctccttttt aggccggatc gccgtgggta aggtcgcgag
      541 aggttccatt aaagaaaatc aacccgtcgc gctgatgcag accgacggta cgatcaggcg
      601 ttccagggtg aaggagctat atactttcat gggtatggga aaaaagaagg ctaccgaggt
      661 catggcgggc gacatctgcg ccgtcgtcgg tctggaagac ttcaatatcg gtgacaccat
      721 cgccgatgcc gagaaccccg aggcgctgcc gatcatcagt gtcgacgagc cgaccatgag
      781 catgctcttc ggcatcaaca actctccttt ttacgggaaa gacggaaagt tcgtgacctc
      841 gcgtcacctt cgcgatcgcc tgctgaaaga gaccgaaaag aatctcgcac tgcgggtcga
      901 ggacacggac ggcgcggaca gcttcctggt ctatggccgc ggtatccttc acctgggtat
      961 cctcatcgag acgatgcgcc gcgaaggctt cgagctcact gtaggacagc cgcaggtgct
     1021 ggtgaaagag atcaatggca aaaagcatga gccatatgag aatctcgttg tcgacgtacc
     1081 gggcgagttc agtggaaaag tcatcgatct cgtgactcag cggaaaggcg aactcctcgt
     1141 gatggagaca aagggcgaaa tgcagcatct cgaattcgaa atacctagcc gggggctcat
     1201 cggtctgaga agtacgatgc tcaccggcac cgctggcgag gccgtaatgg cccaccggtt
     1261 cctggaatac aaaccctgga agggccccat ccccgggcgc aacaacggcg tgctgctcag
     1321 caagaaccag gaaaagacta cggcctactc catcgataag cttcaggatc gcggtacctt
     1381 tttcgtcgac cccggcgaag acgtctattc cggccagatc atcgcggaac atatcaaacc
     1441 cggcgatctg atcgtcaacg ccaccgaggg taagaagctg accaacatgc gtgcaagcgg
     1501 cagcgatgac gcggtccgta tcgccccgaa gaagcagatg acgctcgaag agtgtatgga
     1561 atatatccag caggacgaat gtatcgaggt gacgccgaaa aagatccggc ttcgcaagac
     1621 gatcctcgac gaggaagagc gtaaaaaata ccagcggatg ctgaaaaccg aagaggtagg
     1681 cggataagtc gtacctttac tgtactatca tgagcaagta cctggtcata ttcctgttct
     1741 ttgcgctcgc gatgggcatc caaccggcca ccgcgcaatg ttctatctgt acgcggacgg
     1801 cgcagcagct cggcgaacgg cccgccaaag ccctgaacaa aggcatcatt tatctggcct
     1861 tcacccctct ggctattttc ttcgtcatcg ggtaccgttg ggcgaggagc gagggtcatg
     1921 gctggtggaa gagcgaggat catgactaag ccgcatcttg tttccatcgt tttgggcacc
     1981 tacaatggcg agaccttttt ggaggagcag cttaggtccc ttctcgtcca gacctacccc
     2041 catctggaga tcgtcgctat cgatgacgga agcaccgatg gcaccgtcgg catcctgaag
     2101 gatttcgcgg cccgcgatcc ccgtataaag atcatcctca acgaaaagaa tcttggcttt
     2161 atccgcaatt tcgaaaaagg ttgtaccctg tcctcgggaa aatggatctc tttgtgcgac
     2221 caggacgact actggtaccc ggacaaagta gagagaatgg tgaatgccat cggcgatctc
     2281 ccgatgatct attgcgactc cgagctctgc gaccagcagc tgcagccgaa cggaaaaagg
     2341 atctccgatc ttgtacgcta ccagtctttc tatgactgcc ggcagctttg cgtcttctca
     2401 cgaatgtatg gccacgccac gctgatcaca cgcgatcttt tcgacagatg ccgtcccttc
     2461 ctcgaaggga taccgcatga cggatggctg gcttttcatg ccactctgta tggaggcgtg
     2521 gcgtatttgc cggaggtgct tgtaaaatac cgtcagcatg ccgccaatgt ctatggcgtc
     2581 gtcggccgtc ggcaaaagaa aaaaaaccgg ccggccagat cggaaaggag gagaaaggaa
     2641 ctggcgcgga taaggctgcg catggatgct ttttataagg cctgtcccgg cgatctcgcg
     2701 ccgcagaaag agctgcttcg ccggctggtc cgctgttacc gtaatttttc accgctgaat
     2761 gatatccgcc gtgtcggcct gttctttgcg aactacaagc tcctgctggc ggtgaagaat
     2821 tattccacct ggcggaaata tttattctgt ctgaagatgt tggtcaagat caagtgatcc
     2881 actcccgcac gaacctgaac agattgaatt ccccattttt cgaaagcgtc tctatctttt
     2941 cggcaagtgt ctgccggtcg atatcctgca tgcgctgttg ctggatgagc aaccaggcct
     3001 gttcggccag caggtgataa cgccgctggg acgcgacgtt ctttttcagg tgttcgccga
     3061 tatggtccgc cagctcgccg atgccctgtt tttgcgaagc gatcgttttc accacgggga
     3121 tatcgcgtcc ttcacgccgg aatgcagggc tttgcatcga acgcaggttg cgaacgaaca
     3181 gatcggcatc gggccggtcg ctcttgttca ctacgaagat atcggccacc tccagcagac
     3241 ccgccttcat cgattggata tcgtcaccgg cctccggtgc aagcacgacg attgtggtat
     3301 cggcaagccc cgcgacggcg atctcgttct gccccacacc cacggtctcg atgaggagat
     3361 gatcgaaggg cgcgtgtttt agcaggttgg ttatctcgat ggcgcgtgga tgcagcccgc
     3421 caggcgagcc gcgtgtggcg aggctccgta tataaacgtc cggatgatta taccagtcgc
     3481 tcatccggat acggtctccc agcaacgcgc cgtaatacaa cgaggatgac ggatcgatac
     3541 aaaggactgc gaccctctgt cgcgccgcga catagtagcc gatcaaagcg tccaccagcg
     3601 tgctcttgcc tgcgccgggg ggaccggtga tgccgatgac tggaatgttg gcgggaggac
     3661 aggtagcaag gaggccggca aagtcgttca cgccgttttc gatataggag atagcacggg
     3721 ccagcgccct gcgatcgccg cgtcgcaacg ccgtccatag ctgatcttcc cgttctgcca
     3781 taggtacaaa tatatgtcaa ccgaagtgac tatctttacc ggcatggcga caactttcat
     3841 catcagccgg acggacgcca tcggagatgt agtgctgacc ctacccgtag caggtgtatt
     3901 aagatcgctc tatccccaat cccatattta tttcctgggc cggagttata ccgaagatgt
     3961 cgtcaaagca tgtgagcatg tagatggatt cctgaactgg gatgaatgga agacccttcc
     4021 cgacgccgat gcggcccgcg ccctggccga taccaaagca gataccatca tccacgtttt
     4081 cccagacagg aggatcgcaa gactggcccg ccaggcaggg atacgtcagc gtatcggaac
     4141 gaccaaccgg ctctatcact ggctcacctg caaccgcctg ataagactat cgcgacggca
     4201 ttcccccttc catgaagcgc agctgaacct gcagctgctg gccccggtgg gcgcggagga
     4261 gctctacccc ctcgcgtcga tcgcaggctt ttatggactg acgcgactgc cggaactgcc
     4321 gtcttccata gcctcgctgc cggcatccgg caaattcaat ttagtattgc atcctaaatc
     4381 gcgcggcagc gcacgtgaat ggggattgga taattttcgg gaactgatcc ggctgttgcc
     4441 gcaggaccga ttccggatct tcatcaccgg taccgcggcg gaaggtcagc tggtgcagcc
     4501 gctgttgcag gaatttccat tggtgaccga tctgacgggc cggctctcgc tgggccagct
     4561 gatgaccttc ctatccgtga cggatggact cattgcggcc agcacgggcc ccctgcacct
     4621 ggccgcggcg ctcggcatcc acgcgatcgg aatttatccg ccgataagac ccatgcatcc
     4681 aggacgctgg gcacccgtcg gacgccgggc taaagttttc gttcgggaag ccgaatgcga
     4741 ggcttgccga aaaacggcgg actgcgtctg catgcgcggg atccggccgc aacaggtggg
     4801 ggactatctg ctgacgctga catcccccgt tcgccggggg cgaagtctct aaaaaaggta
     4861 tttttgcatt tatgactaat ttcattcctg tatttcccct gggcattgtc gtatatccgg
     4921 gagaaatggt gaatctgcat atcttcgagc cgcgttacaa acagctgatc aacgaatgct
     4981 acaccgaagg aaaacccttc ggtatcccga cggtcatcga caacaggctg agtgaaatgg
     5041 gtacgctggt gcgggtcacc gaggtggtga agacatatga caacggcgag ctggacatca
     5101 agacgctggg tctacgtgtc ttccgggtgt tggagttgat caaaaatgtt ccggacaagc
     5161 tcttcagcgg agcaatcgtg aattatcccg ataatatcga agggcctgtg aaaagagacc
     5221 tgatgcaacg cgtggtcaat gccatccgcg agcttcaccg gctgctcaac atcgagaagg
     5281 atttccataa gcccgatgaa gaattggcga gttatgatat tgcgcaccat gtaggtcttt
     5341 cgctcgaaca ggaatacgag ctgctgggcc tgctccggga agaacaaaga caggaatata
     5401 tcaaacgtca tttgggaaag gtgctcccgg tgatcgctga aatggaaacg ctgaaagaaa
     5461 gagtgaagct gaacggacat ttcaaaaacc tgtcttcctt caaattcgaa gactaatatg
     5521 gctaccaccc tttgtttcga tttcggcaac acgcggatga aatgcggcgt gttcacagac
     5581 ggcaattttt cggaagagct ggtgttgcac agcgacaccg atgaaaccat cgcgggcctc
     5641 cttgagaaat accgccctga acggtccatt ctgtcatcgg tgatccatca taatcccgga
     5701 atggagaagc tgctgggtgc gggttccagg ttccacctgc tcggtcacca atcgcggctg
     5761 ccggtcacca cgcctgtcgg taaacccgaa accatcgggg ccgatcgtct tgcgcttgtc
     5821 gtggccgccg tcaccctctt ccccggttcc aacaatcttg tcatcggcct gggctctgcc
     5881 atcacatata attatgtcaa taagtataag gaattcatcg gtggcggcat ctctcccgga
     5941 atggaaatgc gtttccgcag cctgcacgaa ttcacggcca aattgcctct cgtcaaagcc
     6001 gactggaatt tcccgctggc tggttatgac acccgcacca atatcctcag cggcgtgatc
     6061 cttggaatgg ccaaagaggt agaaggtatc atttccgcct atgaagaaaa gtatgcaaat
     6121 tttaacgtgc tgttaagcgg gggagacgcc gtttatttta cccgttacct gaaaaagaag
     6181 atatttgccg atccatattt aatctataaa ggtctgtatg ccatcagtgt cttcaatgat
     6241 taagggtttg atcgggagcc gggcgatcag gggaggtgcg ggctggccgc tcctgctgct
     6301 catcgccgtg ttagcggtat cgtgcggcgg aaagaccgct gtgcgacctt ccaacctcgt
     6361 gcaccagatc atccccgaag aagccgacag cattaccagc cttctgagcg agccggtaga
     6421 ttccggcagc aagctcgcgg caagggtaaa aggcaagctg acctctccct ttatgctacg
     6481 gtatgaccgg accgattcgc cctatgcgga atttccccga agcctgcatg tcgattttta
     6541 caacgactcc atgcagatcg aaagcaagct
//
LOCUS       DASYSV010000375         6820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1870441, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000375 DASYSV010000000
VERSION     DASYSV010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870441"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VGQ51_11500"
     CDS             complement(<1..1490)
                     /locus_tag="VGQ51_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEV9037243.1"
                     /translation="MKRSFFTVLFFALSLATFAQGRDEFNGPYNSWADVKKRFGAHGN
                     GKDDDTKALQNALDGLSNPVTGTNLGAGAYVVVYLPAGTYCISSTLQLKGKIGVTIIG
                     EDPSRTIIKWIAKDSGTMLLCNGSAYFKIGRLTWDNGGVKGMEGIGVHWLTKWNNGTT
                     RSFASLNIELSDMVFTPGFRYGIGGGTVPGPGTGANDSELAIRRCKFQNCTVSGVEIL
                     GFNAIDYWIWDCSFIGCNYGIHCTKGTYHAYRCYFSGSTFSDVHNDQGYYVSVRGCYS
                     ENSNAFSLDDGSSCNPFKRIFQDNVILKPKFLPVEFYHLGKITLMGNQFSTTVDTTFK
                     ISINTKSWCPGIYEVLSLHNSYGYRSPIRIASNPQKLYSFGDTYNPVVRATAAAFLKT
                     MDQTPARVSRKIFEVPAGADAAAIQSLIFQAAALKGQRPIIHFGVGTFYLDRPLLVPP
                     GCDMQLTGDGLIYASVITAKQPASSTASPARPAQASLPALLVVKGPS"
     gene            complement(1487..3343)
                     /gene="asnB"
                     /locus_tag="VGQ51_11505"
     CDS             complement(1487..3343)
                     /gene="asnB"
                     /locus_tag="VGQ51_11505"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV9037244.1"
                     /translation="MCGIAAILDRKNGTIPQELIGKMTNAMSHRGPDADGFFCENEIA
                     LGHRRLSIIDLSTAANQPFFDNSGRYVLVFNGEIYNYAEIRPLINDYEFHTNGDSEVL
                     LAAYIKWGPGCLSYLRGMFAFCIWDKKEKQLFMARDRMGVKPLYYYLDDEKLLIASEI
                     RAILATDLVPRKLSEPALLEYFAYQSVSFPFSIIDGIRQLEAGAWMSVRNDSVEVQRY
                     WEVDRPPVSDHDDPVRVKRRIKELLLQSVKRRLVSDVPVGAFLSGGIDSSAVVGLMAE
                     ISAAPNTFTISFEEKEYDESVYAEMVSRKFNTNHTRILLRPTVFLDELENSLDAMDTP
                     SGDGVNTYVVSKTIRQHGMTVALSGVGGDELFAGYPFFNQYLQLHGKKALWKSPALLR
                     KALAAVMAPGNAHGGKGKIRQLLTADSWFIDRSYPVFRQILSPALFRQLTRLPAGDTA
                     VARELSARKSTLSGLPLLSQVSAAEFLGYTQHTLLKDTDQMSMAVSLEVREPFFDQDL
                     VEFVLSVPDTQKTPVYPKSLLVESLKPLLPDEIVFRKKQGFTFPWEVWMKKELRTFCD
                     RRIRNMAQRPFIREEKLLSFWQAFLDGDAHIRWPEIWLFVVLEYWLEKNRVS"
     gene            complement(3319..4329)
                     /locus_tag="VGQ51_11510"
     CDS             complement(3319..4329)
                     /locus_tag="VGQ51_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV9037245.1"
                     /translation="MPTLCLLFRRPAQRFHSIERVFGQLDDQLGEEFRVEKTFLPYYS
                     SSIFNLLRNILFTRRRSADIFHVTGDVHYAVWGLPRGRTVLTIHDLVFLYRPGGIKRR
                     LFKWLFLDMPVRHCGLITTISEATKKDILANTKCAPEKIVVIPDPVDQRMRYVPAVFD
                     ETNPVILFIGSTPNKNLPGVIEALEGVPCTLNIVGKPSPDQVDKLNERKIRFTNRYDL
                     SDEELIAQYIGADLVLFPSTFEGFGLPVVEAQKAGRPVITSDLDPMREVAGGAACLVD
                     PYKPESIREGVLKVIRDKAYREQLVEKGFINAERFSPGDIGSRYSAAYKTLLSCAASQ
                     PF"
     gene            complement(4335..5792)
                     /locus_tag="VGQ51_11515"
     CDS             complement(4335..5792)
                     /locus_tag="VGQ51_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037246.1"
                     /translation="MQAETMKSRYYLTSILEAAIFLLSLVTDWYSPLVITLMVIVLLS
                     MLNRLGKGIVLREIIALHICFVCLVAPLLGYDIFNQNNRLARMFIKYMLVPQPEYYGF
                     ALPAVSGFITAICWPIGTRKYDDKGKFIHQLIDRAKAILEQQKKAPLYLLIIGTITFF
                     FSAYLPVAVRFAFELLFFASFAGFLYVYYQQRSRYRSVVLTVFTIVIVAAAVTSGIFT
                     IVAYMSLTLFSFFFLGRRASLFKKILVLLFGVLVLLIIQSVKPTYRIMTWGKSYAGNK
                     AALFGDLVVEKLSNPNLISAEAFFPIYERINQGYNVTLVMRRFPQVVPFDMGSNLLRN
                     LASALVPRFLWPDKPNAGGKYNMAYYAGFYINGWSTNVGPLGEAYGSFGVTGGIIYMI
                     LLGAFIRWAYKLVFKLANKMPLLIFWIPVLFYEATYAAETDTLQIMNSLIKAAFFVWL
                     LYKLFPTLFGEERKNTRRRVRTALPYTTGPARFEP"
     gene            complement(5806..6633)
                     /locus_tag="VGQ51_11520"
     CDS             complement(5806..6633)
                     /locus_tag="VGQ51_11520"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEV9037247.1"
                     /translation="MDALLRFLPEFKGKHRLARLLLKNDLASKKDVMVKGRYGCRYLL
                     PNLVENVSFEIYINGIYEKASSDFIVSRLGEGAVFLDIGANIGAIGIPVRKQRADCTV
                     VCVEAAPWLNDYLGKNLELNGFGDIRKINKALYHTDNEILNFYSPSEKFGKGSLSPVY
                     TDQAVKVCTVTVDTLLKELHLPKVDFIKVDVEGYEYAVFQGMEQLLSGKTPPDIFFEF
                     EDWAEQAAGVTPGSAQRLLLDKGYELFTLGPNGNLEPLSGALSKGSLMLFASVKRSR"
BASE COUNT         1597 a         1754 c         1896 g         1573 t
ORIGIN      
        1 gaaggccctt tgacgacgag cagggcgggc agcgaggctt gtgcgggacg tgcaggggaa
       61 gccgtggatg acgcgggttg tttggcggtg attacggaag catagatgag cccgtcaccg
      121 gtgagctgca tgtcgcaccc ggggggtacc aggagcggac ggtcgagata aaacgttccg
      181 accccgaaat ggatgatggg tctttgcccc ttcagggccg cggcctgaaa gatcagggat
      241 tggatagcag ccgcgtccgc gcccgccggc acctcgaata tttttcgcga aacccgggcg
      301 ggcgtctgat ccatggtctt caggaaagcg gcggccgttg cccggacgac ggggttatag
      361 gtgtcgccga aggaatatag cttctgtgga ttggaggcga tgcggatcgg cgatctgtac
      421 ccatagctgt tgtgcagcga caatacttca taaatgccgg gacaccagct tttcgtgttg
      481 atgctgatct tgaatgtcgt atcgacagtg gtcgaaaact ggttacccat cagggtgatc
      541 ttgcccaggt gatagaattc caccggcagg aacttaggct tcaggatgac attatcctgg
      601 aagatgcgtt tgaaaggatt acagctgctg ccgtcatcga gcgagaaggc gttcgaattt
      661 tcggaatagc aaccccggac cgacacataa tagccctggt cgttatgcac gtcgctgaag
      721 gtggatccgg aaaaatagca ccgataggca tgataggtgc ccttcgtgca gtgaataccg
      781 taattgcatc cgatgaagct gcaatcccag atccagtagt cgatggcatt gaagccgagg
      841 atctccactc ctgaaacggt gcagttctgg aatttacatc gtcggatggc gagctcggaa
      901 tcattggcgc ccgtgcccgg tcctggaacc gtcccgcccc cgatgccata ccggaagccg
      961 ggagtgaaga ccatatcgga caattcgatg ttcagcgatg cgaagctgcg tgtcgtgccg
     1021 ttgttccatt tcgtcaacca atgaacgccg atgccttcca tgccttttac tccgccgttg
     1081 tcccaggtga ggcggccgat cttgaaatag gcggagccgt tgcagaggag catcgtgccg
     1141 gagtctttgg ctatccattt gatgatggta cgggaagggt cttcgccgat gatggtcaca
     1201 ccgatcttcc ctttgagctg gagagtcgat gaaatacagt aggtaccggc ggggagatag
     1261 acgaccacat aggcgcccgc gccgaggttg gtaccggtaa ccggattgct taatccgtcg
     1321 agcgcgtttt gaagggcttt ggtgtcgtcg tcttttccgt tgccgtgtgc gccgaatctt
     1381 tttttgacgt ctgcccagct gttataggga ccattgaact cgtctcttcc ctgtgcgaaa
     1441 gtcgcaagtg aaagcgcaaa aaagaggacc gtaaaaaatg atcttttcat gatacccggt
     1501 ttttttccag ccaatattcc aacaccacga acagccagat ctcgggccac cggatatgcg
     1561 cgtctccgtc gaggaaggcc tgccagaagg acaggagctt ttcttcccgg ataaatgggc
     1621 gttgcgccat attcctgatg cgcctgtcgc aaaaagtccg gagctccttt ttcatccaaa
     1681 cttcccaggg gaaggtgaag ccttgttttt tacggaatac gatctcatcg ggaagcagcg
     1741 gtttcagcga ttccaccagc aggcttttgg gatatacggg tgtcttttga gtatcgggca
     1801 cggagagtac gaactccacg aggtcctggt cgaaaaaggg ttcgcggacc tcgagcgaaa
     1861 ccgccatgct catctgatcg gtgtccttta acagggtatg ttgggtatag cccaaaaatt
     1921 ccgccgcgga aacctggctc agcaagggca gcccggataa tgtgcttttt cgcgccgaga
     1981 gttcgcgggc tacggcggta tcgccggcgg gcagtcgggt cagctgccgg aaaagggcag
     2041 gcgacaatat ctgtcggaaa accggatagc ttcggtcgat gaaccaggaa tccgcggtta
     2101 gcagctgtct gatctttccc tttcccccgt gggcattgcc gggcgccatg actgcggcca
     2161 gtgctttccg cagcagtgcg ggtgacttcc acagcgcttt cttcccgtgg agctggaggt
     2221 attggttaaa aaaaggatag cctgcgaaga gctcgtcacc acccacgccg gaaagcgcca
     2281 ccgtcatccc atgttgccgg atggtcttcg acacgacata ggtatttacg ccgtcgccgc
     2341 tgggtgtatc catagcatcg agcgaattct ccagctcgtc caaaaaaacg gttggacgaa
     2401 gcaatatcct cgtatgattc gtattgaatt ttcgcgacac catttcggca tagaccgact
     2461 cgtcatattc tttttcttcg aatgaaatgg tgaacgtatt cggggcggcg cttatctcgg
     2521 ccatgagacc gaccactgcg ctggaatcga tgcctcccga caggaaggct cctacgggaa
     2581 cgtcgctgac caggcggcgt ttgacggatt gcaggagcag ctcctttatc cttcgtttga
     2641 cgcggacggg gtcgtcgtga tcggagaccg gcggccggtc tacttcccag taccgctgta
     2701 cctcgaccga atcgttcctg acagacatcc aggctccggc ctccagctgt cgtattccat
     2761 cgatgataga gaatgggaag ctgacgcttt gataagcgaa atattcgagc agggccggct
     2821 cggagagttt tcgcggcacc agatcggtcg cgaggatcgc gcggatctcg gaggcgatga
     2881 gcagcttttc atcgtcgaga taataataga gcggtttgac ccccatccgg tccctcgcca
     2941 tgaacagctg tttttccttt ttatcccaga tgcaaaaggc gaacattccc cggagatagg
     3001 aaagacagcc gggaccccat ttaatatagg cggccagcag aacttcggaa tcgccgttcg
     3061 tgtggaactc gtaatcgttt atcagcggcc ggatctccgc ataattgtat atctccccgt
     3121 tgaagacgag gacgtatcgc cccgagttgt cgaagaaagg ctggttggct gcggtggaca
     3181 gatcgatgat ggacagacgg cggtgaccga gtgcgatctc gttctcgcag aagaacccgt
     3241 ccgcgtcggg gccgcgatgc gacatggcat tcgtcatctt cccgatcagc tcctgcggaa
     3301 tggtcccgtt ttttctatct aaaatggctg cgatgccgca catgataaaa gcgttttata
     3361 cgcggcgcta taccggcttc cgatgtcgcc gggtgaaaat cgttccgcat tgatgaatcc
     3421 tttttctacc agctgttccc gatacgcctt atcgcggatg accttcagca ctccctcccg
     3481 gatcgattcc ggtttatagg gatcgaccag gcaggctgcg ccgcctgcca cttctctcat
     3541 cgggtcaaga tcgctcgtga tgacgggacg gccggctttt tgcgcctcga cgacaggcag
     3601 gccgaaccct tcgaaagtag atgggaataa aacgaggtcg gccccgatgt attgcgcgat
     3661 gagctcctca tcggaaagat cataacgatt ggtgaaccgg atctttcgtt cattgagctt
     3721 gtctacctga tccggcgatg gttttccaac gatattgagt gtgcagggga cgccttccag
     3781 cgcctcgatc actcccggaa gatttttatt gggcgtgctg ccgataaaga ggatcacggg
     3841 gttggtctca tcgaacaccg ccggaacata gcgcattcgt tgatctaccg ggtccgggat
     3901 gaccacgatc ttctcgggag cgcatttcgt attggcgagg atatctttct tcgtagcctc
     3961 cgagatggtc gtgatcaggc cgcaatgtct gaccggcatg tcgaggaaca gccatttgaa
     4021 caagcgtcgt ttgataccgc ccggtcggta tagaaagacc aggtcgtgga tcgtcaggac
     4081 ggtccgcccc cggggcaggc cccaaacggc ataatggaca tccccggtga catgaaagat
     4141 atccgcggaa cgacgccggg tgaacaggat gttccgtaac aggttgaaga tcgaactgct
     4201 gtaatacgga agaaaggtct tttcgacccg gaattcctct cccagctgat catccagctg
     4261 accgaatact ctttcgatgc tatgaaatcg ttgtgcgggc cttctgaaga gcaaacataa
     4321 tgtaggcata atgatcaggg ttcgaaacgg gccggacccg tcgtatacgg tagagcagtc
     4381 cgaacccggc gtcgcgtgtt ctttctttct tctccgaata gggtcgggaa caatttgtac
     4441 aaaagccaga cgaagaacgc ggccttgatc agggagttca tgatctggag agtatcggtt
     4501 tccgcggcat aagtagcctc atagaataat acgggtatcc agaagatcaa taacggcatt
     4561 ttgttcgcca gtttgaacac cagcttgtag gcccatcgga tgaaggcgcc gaggagtatc
     4621 atgtagatga tgccgccggt tacgccaaaa ctgccatagg cctctcccag cggtcccacg
     4681 ttggtggacc agccgttgat ataaaacccg gcataataag ccatattgta tttcccgccc
     4741 gcgttcggtt tatcgggcca caggaacctg ggaacgagcg cggacgccag gttgcgcaac
     4801 agattgctac ccatgtcaaa cggcacaacc tgtggaaacc gccgcatgac cagcgtcacg
     4861 ttataaccct gattgatccg ctcataaatg gggaagaagg cctccgccga aatcagattt
     4921 gggttcgaca gcttttccac cacgagatct ccgaataaag cagccttatt gcccgcatag
     4981 ctttttcccc aggtcataat gcggtaggta ggctttacgc tttggatgat cagtaagacc
     5041 aacacaccga acagcaaaac gagtatcttc ttgaaaagac tggccctgcg gccgaggaag
     5101 aaaaaagaga agagggtaag gctcatgtag gctacgatgg tgaaaatacc ggaagtaact
     5161 gcggcagcca cgatgactat ggtgaagacg gtaaggacga cacttcgata acgggatctc
     5221 tgttggtaat agacgtacag gaagccggca aaggacgcaa aaaaaagcag ctcgaaggcg
     5281 aaccggaccg ctacgggcag ataggcagag aaaaagaatg tgatggtgcc tatgatgagc
     5341 aggtacagag gtgccttttt ttgctgttca aggatggctt tcgcccgatc gatgagctga
     5401 tggatgaatt tacccttatc gtcgtatttt ctggttccga tgggccagca gattgcggtg
     5461 atgaagccgg agactgccgg aagggcgaag ccataatact ccggctgggg tacgagcatg
     5521 tattttataa acatccgggc cagccggtta ttttggttga atatgtcata tccgagcagg
     5581 ggcgccacca ggcacacaaa acagatgtgg agcgcaataa tctcgcggag tacgatgccc
     5641 ttccccaagc ggttcagcat cgacaggaga acgatgacca tcaaagtgat caccagcggg
     5701 gaataccaat ccgtcacgag ggaaagcagg aaaatggcag cttccaatat gctggtaaga
     5761 taatatcggc tcttcatggt ctcggcttgc atagacaaag gtaagctatc gggatctttt
     5821 gacggaagcg aaaagcatga ggctaccctt gcttagggcg ccggacaaag gttcaaggtt
     5881 accgttgggt ccaagcgtga acagttcgta tcccttgtcc aggagcaatc gctgggcgct
     5941 gcccggagta acgcccgctg cttgttccgc ccaatcttcg aattcgaaaa agatatccgg
     6001 cggtgttttt ccactcagca gttgctccat cccctggaaa acggcgtatt catacccctc
     6061 cacgtcaacc tttataaaat cgaccttcgg gagatgcaat tcctttaata gcgtatcgac
     6121 agtgaccgtg catactttta ccgcctggtc ggtatagaca ggggataagg agcccttacc
     6181 gaacttctct gaaggcgaat aaaagttaag gatttcgtta tccgtatgat atagggcctt
     6241 attgatcttc cttatatcgc cgaaaccgtt cagttcgagg tttttcccga gatagtcatt
     6301 gagccagggc gcagcctcta cacatacaac agtgcaatcg gctctttgct ttcgaacagg
     6361 gatgccgatg gccccgatgt tggcgcctat atccaggaag acggcccctt cgcccaacct
     6421 gcttacaata aagtcggagg aagctttttc gtatatgccg ttgatataaa tctcgaagct
     6481 aacattctcg acgaggttag gtaaaaggta cctgcaacca taacggccct tgaccatcac
     6541 gtcctttttt gatgccaggt catttttcaa aagcagcctt gccagtcggt gcttaccttt
     6601 gaactccggc aaaaatctca acagagcatc catagaattt atatttattt attgtaagca
     6661 ttggtttccc ctatgcccga atcgatcaaa gccgccagtt gtgccgtaac ggcccgggcc
     6721 gaataccggt cgaactcctg catattcacc tgccctttat cgaaggccgc gtggaaggtt
     6781 ctgaattgtc gcagtgtggc catgaattcc tgcctgatga
//
LOCUS       DASYSV010000376        10030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2377626, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000376 DASYSV010000000
VERSION     DASYSV010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2377626"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VGQ51_11525"
     CDS             complement(<1..995)
                     /locus_tag="VGQ51_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEV9037248.1"
                     /translation="MNYKSFKKRVSSIGVAAVAGSILLGGCTKRFEEYNTNPYGISNA
                     DMNADYKLLGQPLEQVQQGIYVSQPAWDTQLQQNLIGDNYSGYFGSPTPFLGNQNNMT
                     YALVDGWNGQPWADAYNDVMAPVQQVLKNAGTQYPTFYAWAKILRVEAMHRLSDIYGP
                     IIYTHYGQVNTDGSITYDSQKDAYYAFFSDLDSAIAVLTPLAQSNAASTITNFDQVYG
                     GSYAEWVKFANTLKLRLAIRISLIDPAKAKTEGESALANPVGLLTTPTDNFTINTGTF
                     THPINVMNNSWGDCRFGAPLGSYLTGYNDPRTPKYALPATDPAVAGKVIGVRNGV"
     gene            complement(1021..4566)
                     /locus_tag="VGQ51_11530"
     CDS             complement(1021..4566)
                     /locus_tag="VGQ51_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9037249.1"
                     /translation="MRKTNCNLRSLGFLLSMQVCFSVAAFSQSSYAYAPKTVGVAGQK
                     ANVKDQPEEGMQKQTLFNVLKELNRTKGIYFLFSDGSLRTRIVNALPAGNVSVERTLQ
                     QVLMNTGLKFKKVNEKTFVILSASSATSATDAKPVDFTQNMVISPVAENLVIAPAFEP
                     VIGKVQGPDDAAISGVSVTVRGTKRGTSTNAAGEFTIDAKPGDVLVISYIGYRTQEVT
                     VGSGSLNIKLVALDLQLNEVVVTALGIQHKAKDLTYATQKLANSDLTTVKDANFVNSL
                     SGKVAGVTITKSASGLGGSARVVLRGNKSTQNNQVLYVIDGIPMANYSPAQPGDVWGQ
                     ASGAGSSGRDGGDGISNINPDDIESITILKGASGAALYGSAAANGVIVITTKSGKAGR
                     TRVSISSDVTAEKPMYYPETQFKYGQGLGGTAVTDTTNENSWGPVVNAPDHVKPYFQT
                     GLTEVTTVSLSGGTEKAQTYFSYGNTSSKGIQPTTSFIRHIFNFRETQKFLNDHLTAD
                     ANLNFIDQEADNRPVSGLYANPLTGLELFPRGLNFNQYKTGYEYFSPLRNTYLQNWFD
                     INYDKSFTGEDHEQNPYWVLNRMPRVDKRDRVIGNLTLKYKFNDWLTLQARGNIDKSW
                     DVYDSRMYAGTQSVQSAFDGRYTYANNVNTQLYGDAILMANKNLSTDWTLQANLGTSI
                     TDTRLTDLDFDTNPTDAEGLSFANKFGLQFIVPSALVSNQFDQRNQLQSVFGSAELGF
                     RQFLYFDLTGRNDWSSTFAFTPTEKSGYFYYSAGANLVLSDAFTMPSGVSFGKVRVSY
                     ARVGNAVNIYSTHPPANTATNQAPYSLVNSKAPLPGTSLRPEDNRSFEAGTEWRFAQD
                     RVGVDFTYYKNNNYRQYMEVPAPLGSGFNTYYLNLGNIQNKGVEITAFVVPVATKDFK
                     WTSTLNYAMNKNLVVRLSDPTVPGALPSNAFILTGAGVNMYQSQIKEGGSWGDIYGYM
                     FARNPKDGTIMVNPDGSAIKATTTGGSDSLGLLGNPNPKYTLGWNNSFDIGKWTVNFL
                     IDGRFGGKVLSVTQAVLDEYGDSKTSGSARDAGGVNIKATNAATGTAFSGALPAQAFL
                     KSVGDRNGLTENYMFSATAVRLRELAVGYNIPTRVSWINSMRISVIGRNLFFFHKDAP
                     FDPEVSMSTGNLLQGIETFSQPSTRSIGASLKIGF"
     gene            complement(4599..5624)
                     /locus_tag="VGQ51_11535"
     CDS             complement(4599..5624)
                     /locus_tag="VGQ51_11535"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEV9037250.1"
                     /translation="MSKKYIGVEDLLLDESFLSWYFRTDDRCILQWEAWMASDPQNRA
                     RARQAVAFLRWLTRADKEILQEQITQSESRLLAKIREAEERVVVPMAGRGVRWRWVAA
                     ASVLLVVAGIYTILKLPRIPPAVHTAYGEIKENRLPDGTTVVMNADSKLTFSPGGKDG
                     KDGWKDGKDREVWLTGEAFFHVAKTPLKSRFIVHLNHFDVIVTGTQFNAVNRGQKANV
                     MLKEGSVILHTDQGKELKMAPGDFVEYRNTGLQKKTAKADSVLAWKEHKLIFYGTPLR
                     KLVEVIEEDYGVKVLIKGDSVGSKPIYGILSNDNLDVLLEALKATGDFAVERQIDDTI
                     TISDTHP"
     gene            complement(5778..7343)
                     /locus_tag="VGQ51_11540"
     CDS             complement(5778..7343)
                     /locus_tag="VGQ51_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEV9037251.1"
                     /translation="MKKTVTWIFLFVCLLTGSGCRKDFVAINTDPNAISETQINFNDL
                     FTNAELLTSGNSDGNGYEDWRNNLIYSGCMIQHFSSTFSYWVGDKYLFNGNYNSAYWD
                     QNYPNSIADIVAVLNHAGKDSTQANFYQICRIFRVFMFQRLTDMYGDCPYTQAGLGYI
                     GGVTAPKYDRQQDIYADLLRELQAAASALDPSRPNTIGAADLLYGGDPVGWKRFAYSE
                     MVRLAMRMSKVDSVSAQQWVKTAVQGGVMTNNGQNAIVHHQNVPGTPVPNGNGLILLG
                     NDPNGYRLNTTFVNFLKSTQDPRLVYLATVCANPAIASDKGDTSWSRQLGQPGGYDAP
                     NSGGTYDLTKAPGWTGNQNAYSVVNRYTFARLDAPTFFLTTGETGLMLAEAAQRGWIG
                     GDPAQYYGSAVRAAMLQLAQQAGGGPADSLVGAWLTAHPYNPADGLEQINDQYWIAGF
                     MDENECFANWRRSGYPVLAPVNYPGNVTGGTIPRRFTYPPSEASANMVNYNDAVSRLA
                     GGDKMTSRVWWDR"
     gene            complement(7348..>10030)
                     /locus_tag="VGQ51_11545"
     CDS             complement(7348..>10030)
                     /locus_tag="VGQ51_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9037252.1"
                     /translation="PYGSSRVLIRGNASLSGNNQPLYVVDGIPYDNTTQSSAGQYGGM
                     DLGDGLSNINPDNIESVLILKGVAASALYGYRGGNGAILIRTKSGSRTHGIGVQLNDN
                     FTANPVVDERDYQYVYGQGLSGLKPVTEPMALAAPYYSWGAKLDGSPAVNFLGNTVTY
                     SPAKENFRNFYRTGLTNQVSLALTGANSKGHFRLGITDLHLAAAVPNSTMQQQGFNLN
                     SSYFVTNRLQLDLTADYVFEQVGNRASLSDDPGNVNAAPLYVANSFDIRWMKTHTVTS
                     SGIEWMPGNSDPYFENPYYIAYDYRNSTDRNRLTGGLTLKYNITGWLYLQGQASRDGY
                     QFNVTQIVPSGVPYTRSDGVHGGNLTQYEVNFHEVNVSGMVGVQKKFGERWAFNADAG
                     VNRVDNSNTIQGIGAVPGSTYRAAGPFQSAGDYSAADIVNKPYSDFLLRYRVNSVYGS
                     ADLSYKDYLFLTATARNDWFSTLNIRTDHYLYPSMSGSWLFSETWRLPSWVTLGKLRA
                     SYATSSNGTEPYQNTLSYGIRGGYTISGQTLGYIASGSLPNADLRPVSIAEKELGLTA
                     RFLHDRAGIDVALYNKQTTNDIVKVSVSPTSGYYVYIENIGRIRNKGVELQFDVSPVK
                     EAQYGWNVSFNYAVNNNKVLYIGDQPSIVINGSYPRWGSEVSISNVVGLPYGQIMGYA
                     YKRDAKGRILISDGISNPAPAGEPEQTGVIPLGSTVYRQMGGMTNTFRWHALTLSVLV
                     DFKYGAKLYSGTNLLLDYYGLQKNTLAGREAGYVGKGVMEDGHPNTIAVPAQQYWQDI
                     SAGGSDHIAEEFVYDASFIKLRSAVLTWTAPPGMLKTKIVKGLSFSAVGRNLAILMKH
                     VPNVDPESSINNTNGQGLELSGYPATRSWGFNMNIKF"
BASE COUNT         2139 a         2893 c         2646 g         2352 t
ORIGIN      
        1 ttgaccccgt tgcgcacccc gatcactttg ccggctacgg ccggatcggt ggcaggcaga
       61 gcatatttgg gcgtcctcgg gtcattgtag cccgtcaggt aggaacccag cggcgctcca
      121 aaccggcagt caccccagga gttgttcatc acattgatcg gatgcgtaaa ggtgccggta
      181 ttgatcgtga aattgtcggt gggagtggtc agcagcccga ccgggtttgc cagcgcggac
      241 tctccttccg tcttagcctt ggcgggatcg atcagcgaga tgcggatcgc cagccgaagc
      301 ttcagcgtat tggcgaactt tacccattcg gcatagctgc cgccatacac ctggtcgaaa
      361 ttggtaatgg tagacgcagc gttgctctga gccagcggag tgagcacggc gatggccgaa
      421 tccagatcgc tgaaaaaggc ataataggca tccttttgcg agtcataggt gatgctgcca
      481 tccgtattca cctgcccata atgcgtatag atgatcggtc catagatgtc gctgagacgg
      541 tgcatcgctt ccacacgcag gatcttcgcc caggcataga aggtcggata ttgcgtcccc
      601 gcatttttga gcacctgctg gacaggcgcc atgacgtcgt tataagcgtc ggcccagggc
      661 tgaccgttcc aaccatccac cagcgcatag gtcatattgt tctggttacc caggaagggg
      721 gtcgggctgc cgaaataacc ggagtaattg tccccgatga ggttctgctg cagctgcgta
      781 tcccaggccg gctgcgatac atagatgccc tgctgcacct gctcgagcgg ctgtcccagg
      841 agtttatagt cggcattcat gtccgcgttg gagataccgt acgggttcgt attgtattct
      901 tcgaagcgct tggtacatcc tccgagcagg atggagcctg ctacggctgc cacaccgata
      961 gaagaaacac gctttttaaa agatttatag ttcatatagt atgcattaga gtttttactg
     1021 ttagaatcct atttttaagc tggccccgat gctcctcgtg ctgggctggc tgaaggtctc
     1081 gatgccctgc agcagattgc cggtggacat tgaaacctcc ggatcgaaag gagcatcctt
     1141 atggaagaaa aacaggttcc tgccgatgac ggatatacgc atcgagttga tccaggaaac
     1201 acgcgtggga atattgtaac ccacggcgag ttcccgcaag cgtaccgccg ttgcgctgaa
     1261 catataattt tccgtgagac cgttccggtc gccgacgctc tttaagaagg cctgggcagg
     1321 cagcgcgccg gaaaaggcgg tgccggtcgc ggcattggtg gccttgatat tcacgccacc
     1381 tgcatcacgt gcggagccgc tggtcttgct gtcgccatat tcatccagta ccgcctgcgt
     1441 tacgctcagc acctttccgc caaatcggcc gtcgatgagg aaattgaccg tccatttgcc
     1501 gatatcgaaa ctgttgttcc atcccagcgt atacttagga ttgggattgc ccagcagacc
     1561 cagcgaatcg gaacctcccg tggtcgtcgc cttgatggca ctgccgtcgg ggttgaccat
     1621 gatggtgccg tctttcggat tcctcgcgaa catataacca tagatatctc cccatgaacc
     1681 accttccttg atctgcgatt gatacatatt gacgcccgca ccggtcagga tgaaggcatt
     1741 ggatggcaat gcgccgggca cggtcggatc cgacagcctg accaccaggt tcttgttcat
     1801 cgcatagttc agagtggagg tccatttgaa atccttggta gcgacgggga ctacgaaggc
     1861 cgtgatctcc acacccttat tctggatatt accgaggttc agataatacg tgttgaaacc
     1921 ggaacccaga ggcgcaggca cctccatata ctggcgatag ttgttattct tataataggt
     1981 gaagtccacg cctaccctgt cctgggcgaa ccgccactcg gtgccggcct cgaatgaccg
     2041 gttgtcttcc ggtcgcagag aagtccccgg caagggcgcc ttcgagttga ccagggaata
     2101 gggtgcctgg ttggtagcgg tattggccgg cgggtgcgtg ctgtagatat tgacggcatt
     2161 acccactctg gcgtaagaga cccggacctt gccaaagctg accccggatg gcatcgtgaa
     2221 ggcatccgac agcaccaggt tggcgccggc cgaataatag aaataaccgg atttctcggt
     2281 cggagtgaac gcgaaggtcg aagaccagtc atttcttccc gtcagatcga aatacaggaa
     2341 ttgccggaaa ccgagctcgg cactgccgaa taccgattgc aattgattcc gctgatcgaa
     2401 ttgattgctg accagcgcgc tgggaacgat gaattgcaaa ccgaatttgt tggcaaagga
     2461 aagcccctcc gcatcggtcg ggttcgtgtc gaaatccaga tcggtcaaac gcgtatcggt
     2521 gatgctggta cctaaattgg cctgaagggt ccagtcggta gaaaggtttt tattcgccat
     2581 caggatagca tccccgtaca gctgcgtgtt cacgttattg gcataggtat accgtccgtc
     2641 gaacgccgat tgaacggact gcgtaccggc atacatcctc gagtcataga cgtcccagga
     2701 tttgtcgata ttgccgcggg cctgcagagt cagccaatcg ttgaatttat atttgagggt
     2761 caggttgccg atcacacggt cgcgtttatc gacccttggc atcctgttca atacccaata
     2821 ggggttctgc tcatggtctt ccccggtaaa gcttttatcg taattgatgt cgaaccagtt
     2881 ctgcagatac gtgttgcgca acggcgagaa gtattcgtaa ccggtcttat actgattgaa
     2941 attcagaccg cgcgggaaaa gctccagacc cgtcagggga ttggcgtaca gaccgctgac
     3001 cggacgattg tcggcttcct gatcgatgaa gttcagattg gcgtccgccg tcaggtgatc
     3061 gttcaggaat ttctgggtct ccctgaaatt gaagatatga cggatgaacg aagtggtcgg
     3121 ctggataccc ttgctggacg tgttgccata agagaaatac gtttgtgcct tctcggtacc
     3181 acccgaaaga cttaccgtgg ttacttcggt gagacccgtt tggaaataag gcttgacatg
     3241 atcgggcgca ttcaccacag gtccccagct gttctcattg gtggtgtcgg taaccgcggt
     3301 gccgccgaga ccctgtccgt atttgaactg cgtttcggga taatacatcg gcttttctgc
     3361 cgtgacgtcg gaagaaatac tgaccctcgt cctgcctgct tttccgctct tcgtcgtgat
     3421 gacgatcaca ccattggcgg cggcgcttcc atagagagcc gcgcccgacg cacctttcag
     3481 tatggtaatg ctttcgatat cgtcgggatt gatatttgag ataccgtccc cgccgtcacg
     3541 acccgaagaa ccagctcctg aagcttgccc ccatacgtca ccgggctgtg ccgggctgta
     3601 gttggccatc gggataccat cgatcacata gagcacctgg ttgttctgtg tggatttgtt
     3661 cccgcgaagg accacgcgcg ccgaacctcc gagaccagat gcgctcttcg tgatcgtcac
     3721 gccggccacc ttgccggaca ggctattgac gaagttggca tctttcaccg tggtcagatc
     3781 ggaattggcc agtttctgtg tggcataggt caggtccttg gccttgtgct gaatacccaa
     3841 cgcggttacg accacctcgt tcagctgcag gtccaaagcg acaagcttga tattgagact
     3901 gccgctgccg acggtcacct cttgcgtcct atagccgata tacgaaatga ccagcacatc
     3961 gccgggtttc gcatcgatgg tgaattcacc ggctgcattg gtggaggtcc cccgtttggt
     4021 acccctgacc gttacgctga cgccggaaat ggccgcgtcg tcaggaccct gcaccttgcc
     4081 gattaccggc tcgaaggccg gtgctatgac caggttctcc gccaccggcg agatcaccat
     4141 gttctgcgtg aaatccacag gcttggcgtc cgtcgcgctc gtggccgaag aggccgatag
     4201 aatgacgaag gtcttttcat tgaccttctt gaatttcaac ccggtgttca tcaatacctg
     4261 ctgcagcgtc ctttccacgc tcacattccc tgccggcagg gcgttgacga tcctggtccg
     4321 cagcgacccg tccgaaaaaa ggaaatagat ccctttggtc ctgttcagct ccttcagcac
     4381 gttgaaaagg gtctgtttct gcatcccctc ttccggctga tctttgacgt tggctttttg
     4441 cccggctacc ccaactgtct tcggcgcata cgcgtaggaa gactgcgaaa aggcggctac
     4501 ggaaaagcac acttgcatgc tcagcagaaa gccgagcgaa cgaagattgc aattcgtttt
     4561 tctcatttta agcagctttt gtttgataat gaaaggtttt atggatgtgt gtccgatatg
     4621 gtaatggtat cgtcgatctg tcgttccacc gcaaaatccc ctgtcgcctt cagcgcctcc
     4681 agcaatacgt ccaggttgtc attggaaagg atgccataga tcggcttgct gcccaccgaa
     4741 tcccccttta tcaagacctt tacgccataa tcctcctcga tgacctcgac cagctttcgc
     4801 aacggcgttc cgtaaaaaat gagcttatgc tctttccagg ccaggacact gtctgccttg
     4861 gccgttttct tctgcaaccc ggtattgcgg tactccacaa aatcgccggg cgccattttc
     4921 aattcctttc cctgatcggt gtgcaggata acacttcctt ctttcagcat gacattggct
     4981 ttttgccccc ggttgacggc attgaattgc gtgccggtga cgatgacatc gaaatggttc
     5041 aggtgaacga tgaaacgact cttgagtggt gtcttcgcga catggaaaaa tgcctctccc
     5101 gtcagccaca cctccctgtc cttcccgtcc ttccagccat ccttcccgtc tttcccgccg
     5161 ggagaaaagg tcagcttgct gtcggcattc attacaacgg tcgtaccgtc gggcagacgg
     5221 ttctctttga tttcgccata ggcggtatgt acggcgggag ggatacgcgg gagcttcaga
     5281 atggtataaa tgcccgcgac gaccagcaga acggacgcgg cggcgaccca ccgccatcgt
     5341 acacctctac cagccatagg gactaccacc cgctcctcgg cctcgcggat cttcgcgagc
     5401 agcctgctct ccgattgggt gatttgttcc tgtaggattt ctttatccgc gcgcgtcagc
     5461 caacgtagga aagcaacggc ctgccgggcc cgtgcccggt tttgaggatc ggaagccatc
     5521 caggcctccc actgcaggat gcagcggtcg tcagtccgga aataccagga aagaaatgat
     5581 tcgtccagca atagatcctc gaccccgata tatttttttg acatggcagc aatggtcctc
     5641 cgggggatat gtgcccagac cgccgccttt gtaatgtgat tttagtaaaa atcttttgcc
     5701 cctcgcccaa ccgggaggtc aacgaaggcc gccgccctct gttcgcccgc ctttctgtca
     5761 tagaccgacc ggtctatcta ccgatcccac cacacccttg aagtcatttt gtcgcctccc
     5821 gcgagcctcg agaccgcatc attatagttg accatattgg ccgaagcctc actgggtgga
     5881 taagtaaagc gtcgcgggat cgtcccccct gtcacattcc cgggataatt caccggcgcc
     5941 aacaccggat aaccactcct ccgccaattg gcgaaacatt cattctcatc cataaaacct
     6001 gcgatccagt attgatcgtt tatctgttcc aacccgtccg cgggattata cggatgcgcg
     6061 gtcagccagg ccccgaccag cgagtcggca ggcccacccc ccgcctgctg cgccaattgc
     6121 agcatcgctg cccgtaccgc ggaaccataa tattgggcgg gatcaccgcc gatccacccg
     6181 cgttgcgcgg cctcggccag catcaaccct gtctcacccg tagtaagaaa aaaggtaggc
     6241 gcatccagcc gtgcaaacgt ataccggttc acaacggaat atgcattctg attacccgtc
     6301 cagccgggcg ccttcgtcag atcgtaagtc cctccgctgt tcggcgcatc gtatcccccc
     6361 ggctgcccca attgacgcga ccagctcgta tcccccttgt ccgaggctat ggcaggattg
     6421 gcgcacaccg ttgccagata gaccagccgc gggtcctgcg tactcttcaa aaaattcacg
     6481 aacgtagtat tcagccgata gccattgggg tcgttgccga gcaggatcaa cccattccca
     6541 ttgggtacgg gcgtacccgg cacattctga tgatgcacga tcgcattctg cccattgttc
     6601 gtcatcacac cgccctgcac cgccgtcttt acccattgtt gcgcgctgac ggagtcgacc
     6661 ttgctcatgc gcatcgccag cctgaccatc tccgaatatg cgaaccgctt ccaacccacg
     6721 gggtcgcctc catacagcag atccgcagcg ccgatggtat tgggacgcga aggatccagc
     6781 gcgctggccg ccgcctgcaa ttccctgagc aggtcggcat agatgtcctg ctgccggtca
     6841 tacttcggag ccgtgacgcc gccgatatag ccgagcccgg cctgcgtata cggacagtcc
     6901 ccatacatat ccgtcagacg ctggaacatg aagacccgga atatccggca gatctggtag
     6961 aaattcgcct gggtcgaatc cttgccggca tggttcagca cggcgacgat atcggcaatg
     7021 ctattggggt aattctgatc ccaatatgcc gaattatagt tgccattaaa caggtactta
     7081 tctccgaccc aataggagaa ggtggaggaa aaatgctgga tcatacagcc cgaatagatc
     7141 aggttgttcc gccagtcctc gtacccgttg ccgtccgaat tgcccgaggt cagcagctcg
     7201 gcattggtga acagatcgtt gaagttgatc tgtgtctcgc tgatggcatt gggatcggta
     7261 ttgatggcga caaagtcctt cctgcacccg ctgccggtca gcaggcaaac aaacaggaaa
     7321 atccatgtta ccgtcttctt catcccatca gaattttata ttcatattga atccccagct
     7381 ccgcgtcgcg ggataaccgc tcagctcgag tccctggcca ttggtattgt tgatgctcga
     7441 ctccgggtcc acattcggga catgcttcat caggatcgcg aggtttcgcc ctacggccga
     7501 gaagctcaat ccctttacga ttttggtttt cagcatcccc ggcggggccg tccaggtaag
     7561 gacggcgctc cgcagcttga tgaagctggc atcatacacg aattcctccg cgatatgatc
     7621 cgatccgccg gcagagatat cctgccagta ctgttgtgcc ggaactgcga tggtattcgg
     7681 atgaccgtct tccatcactc ccttccctac atatcccgcc tctctgccgg caagcgtgtt
     7741 cttctgcaaa ccataataat ccagcaacag gttagtaccc gaatatagct ttgccccgta
     7801 tttgaagtcg accagcacgg ataatgttaa ggcatgccac cggaatgtat tggtcattcc
     7861 gcccatttgc cggtagacgg tagaacccag cgggataacg cccgtttgct ccggctcacc
     7921 cgcaggcgcg ggattggaga tcccatcgct gatgagtatg cggcctttcg catcccgctt
     7981 gtaggcatag cccatgatct gaccataagg gagcccgacc acattgctga tgctgacttc
     8041 gctgccccat cgcgggtaag agccgttgat cacgatactg ggctgatcgc cgatatagag
     8101 caccttattg ttgttgacgg cataattgaa ggagacattc caaccatact gcgcttcctt
     8161 taccggcgag acgtcgaact gcagctcgac tcctttattc cggatacgac cgatgttctc
     8221 gatatacacg taataccccg aagtcgggct gacagacact ttaacgatat cgttcgtggt
     8281 ctgtttgttg tacagtgcca cgtcgatacc cgccctgtca tgcaggaacc tggccgtcaa
     8341 tccgagctct ttttcggcga tgcttaccgg acgaagatcc gcattgggca gactccccga
     8401 ggcaatatag cccagcgtct gcccggagat cgtatagccc ccgcgtatcc cataggacag
     8461 cgtattttga tagggctccg ttccattgga cgacgtcgca tacgaagccc tcagctttcc
     8521 gagagtgacc caagacggta gtcgccaggt ttccgaaaaa agccagctcc cgctcatcga
     8581 cggatacaga taatgatccg tacggatatt cagcgtagaa aaccagtcgt tccgcgccgt
     8641 agccgtcagg aagagatagt ccttatagga gagatccgca cttccgtaaa ccgaattcac
     8701 ccggtaacgc agcaggaaat cactgtaagg cttgtttaca atatcggctg cactgtagtc
     8761 tcccgcagat tggaaaggcc ccgccgcccg ataggtcgag ccgggtaccg ccccgatacc
     8821 ctggatcgta ttcgaattgt ccacccggtt gacgccggca tcggcgttga aggcccacct
     8881 ttcaccgaat tttttttgca ctccgaccat cccgctgacg ttcacttcat gaaaattcac
     8941 ttcatactgc gtaagattgc cgccatgcac tccgtcgctg cgcgtatagg gcactcccga
     9001 agggacgatc tgcgtgacat tgaactggta accgtcgcgc gatgcctggc cctgcagata
     9061 cagccagccc gtgatgttat acttaagcgt tagaccgccc gtcagccggt tgcgatcggt
     9121 ggagttccgg tagtcatagg cgatataata tgggttctcg aaatacggat cgctgttacc
     9181 cggcatccat tcgatgccgg aagacgtcac cgtatgtgtc ttcatccatc ggatatcgaa
     9241 ggagttggca acgtacaacg gtgctgcatt cacattcccg ggatcgtccg acagcgaagc
     9301 tctgttgccc acctgctcga agacatagtc tgctgtcaga tccagctgca gccggttggt
     9361 tacgaaatac gaggagttga gattgaatcc ctgctgttgc atcgtggaat tcgggaccgc
     9421 cgctgccaga tgcagatcgg taatacccag ccggaagtgc cctttactat tggcgcccgt
     9481 cagggcaagc gatacctggt tggtcagacc cgtacggtaa aaattccgga aattctcttt
     9541 tgcggggcta taggtaacgg tattacccaa aaagttcact gcgggcgaac catcaagctt
     9601 tgcgccccag ctgtaatacg gcgcggcgag cgccattggc tccgtcaccg gcttcagacc
     9661 cgagagaccc tgaccataca cgtattgata atccctttcg tcgacgaccg ggttcgccgt
     9721 gaaattatcg ttcagctgaa cgccgatgcc atgcgttctc gagccggatt tggtccggat
     9781 caggatggcg ccgttgcccc cccggtaacc gtaaagcgcc gatgccgcca cgcctttcag
     9841 gatcagcacc gactcgatat tgtcggggtt gatattggaa agaccgtcgc caaggtccat
     9901 accaccgtac tgccccgcgc tgctctgtgt cgtgttgtcg tagggaatgc cgtccactac
     9961 gtataagggc tggttgtttc cgctgagcga ggcattgccc cggatgagca cacggctgct
    10021 gccataaggc
//
LOCUS       DASYSV010000377         4731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_586469, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000377 DASYSV010000000
VERSION     DASYSV010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586469"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VGQ51_11550"
     CDS             <1..1275
                     /locus_tag="VGQ51_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV9037253.1"
                     /translation="ESADEASPGSLDVTGLLRKAVSLFDGGFTAGGLLGNGDSFVFRD
                     AHGIRPAYYYITEDVVVAASERTAIRTVFNVGENEVMELMPGKGLIVKADGSVTIETI
                     LEPKERRACSFERIYFSRGSDEKIYRERIALGYNLSDQILTAIDHDLKNTIFSFIPNT
                     AEVAFYGLWKGMDDYLESIKIQRILSWGNDIREERLVEMLSRKIRVEKVAIKDVKMRT
                     FITEDANRNEMVQHVYDVTYGTVRKGVDTLVIIDDSIVRGTTLRESIIRMLARLEPKK
                     IIVVSSAPQIRYPDCYGIDMSKLGDFIAFRAAIQLTRERGKERCLQELYDRCKELART
                     GQLHTENVVRLVYHPFTAEEIADKIAQLITPPDIQTPVQVIFQTIESLHKACPTNTGD
                     WYFTGNYPTPGGNRVVNKAFINFMEGKNVRGY"
     gene            1540..2181
                     /locus_tag="VGQ51_11555"
     CDS             1540..2181
                     /locus_tag="VGQ51_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9037254.1"
                     /translation="MKKVSIMIVDDHTLIRETWSFLLGKNENFDVVAECGDGERAIEL
                     ARDKRPDVVLLDINMAPMSGFDVLKMIRKYSPGSKIIGVSMHSQPAYAKKMLRLGAKG
                     YVTKNSPRQEMLEAISEVSNNHVYICQEVKNILSDQLLNSDQSNPDINNLSDREMQIV
                     RALKGGLSSKEIASELSISLKTVEVHRHNILKKLKLKNTVSLINFINSQAFDI"
     gene            2183..2683
                     /locus_tag="VGQ51_11560"
     CDS             2183..2683
                     /locus_tag="VGQ51_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV9037255.1"
                     /translation="MKTLLVVRHAKSSWDNTDAGDIHRPLNDRGKRDAPAMAQRLIRM
                     GVTIDRFVSSPAKRALQTAEAFNQTFGGKDKEIKVVPELYHASPATFKELVAGLDNGD
                     QTVAVFSHNPGITTFVNTLTNMQVDNMPTCSIFAVKSNAESWSDFLNTSIQYWFFDYP
                     KSGGRD"
     gene            complement(2680..3435)
                     /locus_tag="VGQ51_11565"
     CDS             complement(2680..3435)
                     /locus_tag="VGQ51_11565"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9037256.1"
                     /translation="MIISDNRYGLVFRGIYCVFITHQLLVKTPFGSWADRLLQRLNYR
                     FIRRFSCCWIPDIAGHGGLAGELSHPVLMPSIPHRYIGLLSRFGEGPQEASGNYLLVL
                     LSGPEPQRTLLEKIIVEQAAGCPHPMILVRGLPQGGTRLSALQGMVIHDHLPAAQLER
                     VIRDAALVISRPGYSTLMDLARLKKNAVLVPTPGQTEQEYLGRRMAALGWAICMEQRG
                     FSLVKALGARCSFASFQEEKDGCLATAIGEALR"
     gene            3987..4235
                     /locus_tag="VGQ51_11570"
     CDS             3987..4235
                     /locus_tag="VGQ51_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037257.1"
                     /translation="MKACNRDLLVLVKDEHMNEKSMERELEQLNDLLFHFETFESFCI
                     AHEVFDVNRHKILHKRDSIQRTVRQRELKPFFFICNKN"
     gene            complement(4232..>4731)
                     /locus_tag="VGQ51_11575"
     CDS             complement(4232..>4731)
                     /locus_tag="VGQ51_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV9037258.1"
                     /translation="VRVGWSFGPSAIIGKMKAIMTHIGSWAPMAEQKATAAYLLQKGA
                     IDVYLKGFRAAVSERLHRIHDGFVALKKEGFKVDVLTPEAAIYLTIKIDLTGQKTNEG
                     RILQDQAAVTSYLLDEAKLAVVPFYAFGASKTSPWYRLSVGTCKLEDVPEMLGKLREA
                     MKKLS"
BASE COUNT         1203 a         1261 c         1190 g         1077 t
ORIGIN      
        1 gagagtgcgg acgaggcctc ccccggaagc ctcgacgtga caggcctgct gcgtaaggcg
       61 gtgtcgcttt tcgacggagg gttcacagca ggtggccttc tcgggaatgg agacagcttc
      121 gtattccgcg acgcccatgg cattcgtcct gcctattact atattaccga agacgtcgtc
      181 gtagccgcca gcgagcgcac cgctatccga acggtattca atgtaggaga gaatgaagtg
      241 atggaattga tgcccggtaa aggcctgatc gtgaaggccg atggttcggt cacgatcgag
      301 acgatcctgg agccgaaaga gcggcgcgcc tgcagtttcg aacgcatcta tttttcccgt
      361 ggcagcgacg agaagatcta ccgcgaacgg atcgctctcg gctataacct cagcgatcag
      421 atcctcactg ccatcgatca cgatctcaag aataccatct tctcttttat accgaatacg
      481 gcggaagtag ccttttacgg tctctggaag ggcatggacg actacctgga aagtatcaag
      541 atccagcgca tcctgtcctg gggtaacgat atccgggaag aacggctggt ggaaatgctc
      601 agccgaaaga tccgcgtgga gaaggtcgct ataaaggatg taaagatgag gacctttata
      661 acggaggacg ccaaccgcaa tgaaatggtc cagcacgtct atgacgtcac ttatggtacc
      721 gtccgcaaag gagtggacac cctcgtcatc atcgacgact cgatcgtcag gggtaccact
      781 cttcgggaaa gcatcatccg gatgctggcg cgtctcgagc cgaaaaagat catcgtcgta
      841 tcttcggccc cccagatccg ttacccggat tgctatggca tcgatatgag caaactgggc
      901 gacttcatcg ccttccgcgc cgcgattcag ctgacccgcg agcgcgggaa ggaacgctgc
      961 ctgcaggaac tgtacgaccg ttgcaaggaa ctggcccgca cggggcaact gcatactgag
     1021 aatgtcgtgc gactcgtcta tcatccgttt accgccgagg agatagccga taagatcgcc
     1081 cagcttataa cccctccgga catacaaacg ccggtacagg tgatcttcca gacgatcgaa
     1141 tcgctgcaca aggcatgtcc gaccaacacc ggtgactggt attttaccgg caactacccc
     1201 acgccggggg gaaacagggt ggtgaacaag gcctttatta atttcatgga aggcaagaat
     1261 gtccggggtt attgatgacc gtttgaccgc agggacccga caaatagccg gatcggttaa
     1321 aaaattctgc atccgcattg cggttctcag ggaaaatctg taatattata tagctaagaa
     1381 cgcccatccc cttaaaagat ctgtctggtt aactttcgct cgattggtat ctgccgtgga
     1441 tagaacttcg ctcctgcggc ccgcaggaac tcgttcgcag gctttcgcct gtctccccgg
     1501 cttctggttg tttgaatgat ttttgaaaac ccaagcgtta tgaagaaagt ctctattatg
     1561 atcgtcgatg atcatacctt gatccgggaa acatggagtt ttttattggg aaagaatgaa
     1621 aatttcgatg tggtggccga atgcggtgat ggcgaacgcg ccattgagct ggccagggac
     1681 aaaagacccg atgtagtatt gctggatatc aatatggccc cgatgagcgg cttcgacgtc
     1741 ctgaaaatga tccggaaata ttcccccggt agcaagatca tcggcgtatc gatgcattcc
     1801 caacccgcct atgcgaaaaa gatgctccgg ctgggcgcca aaggctatgt taccaagaat
     1861 tcgccgcgcc aggaaatgct ggaggcgatc tcggaggtca gcaacaatca cgtctacatt
     1921 tgccaggaag tcaagaatat cctgtcggac cagctgctca acagcgatca gtcgaatccc
     1981 gatatcaata acctttcgga ccgcgagatg cagatcgtcc gtgccctgaa gggaggactt
     2041 tcttccaaag agatcgcgtc ggagctgagc atttcgctaa agaccgtcga agtccaccgc
     2101 cacaatattc tcaagaagct gaaattgaag aacacggtct cgctgatcaa ttttatcaat
     2161 tcgcaggcat tcgatattta atatgaagac cttacttgtc gtacggcatg ccaaaagcag
     2221 ctgggataac accgatgcag gcgatatcca ccggccgttg aacgatagag gcaaacgcga
     2281 cgctcccgca atggcgcagc gtctgatcag gatgggtgtc accatcgacc gtttcgtatc
     2341 cagccctgca aaacgcgcgc tccagacagc cgaagccttt aaccagacct tcggcggcaa
     2401 agacaaggag atcaaagtgg ttcccgaact ttaccatgcg tccccggcga ctttcaaaga
     2461 attggtcgcc ggtttggata acggcgatca gacagtggct gtcttttcgc ataacccggg
     2521 tatcaccacc ttcgtcaata ccctgaccaa catgcaggtc gacaatatgc ctacctgctc
     2581 catcttcgcc gtgaaaagca acgccgaaag ctggagcgat tttttgaata cctctattca
     2641 atactggttc ttcgattatc caaaatcagg gggtcgcgat tagcgtaatg cttcaccgat
     2701 agccgtagcc agacagccgt ctttttcctc ctgaaaggag gcaaaactac accgcgcacc
     2761 caacgccttc accagcgaga acccgcgctg ttccatacaa atggcccaac ccaaggccgc
     2821 catccgccgt cccagatatt cctgctctgt ctgcccgggc gtaggtacca gcactgcgtt
     2881 tttcttcagc cgcgccagat ccatcaacgt actatatccc gggcgtgaga tgacgagggc
     2941 cgcgtcgcgg atcacccgtt cgagctgtgc tgccggtaaa tgatcgtgaa tgaccatgcc
     3001 ttgcagagcg ctaagacggg tcccgccttg cggtaacccc cgaacgagta tcatcgggtg
     3061 cggacatccg gccgcctgct ccacgatgat cttttcaaga agcgtacgtt gcggctccgg
     3121 accggatagc aataccagca gataattccc gcttgcctcc tgcgggccct ccccaaaacg
     3181 cgacaacaac cctatataac ggtgaggtat agacggcatt agcaccgggt gtgacagctc
     3241 gcccgccaaa ccaccgtgcc cggcgatatc cggtatccag caacacgaga accggcggat
     3301 gaatcgataa ttcagtcttt gcaggagccg gtcggcccat gatccgaacg gagttttgac
     3361 cagcaattga tgcgtaataa agacacaata aatgccccga aatacaagcc cataccggtt
     3421 atcggaaatg atcatatcgg gcttttgacg gtccgaaaaa ccctttaacc aagcattttc
     3481 ccgcttgatt cggatcaaga ttttagggat cgacaacatc agccgaaaaa tagtaaaggc
     3541 acgatttttg tcgaatttta tgtcgtaccc aggcagttct acgtaggtca gggatgggaa
     3601 ttccgtctgt aataaggcct tttgcgatcc cctgacagcg acaacgattt cacatctttg
     3661 atttaataat tccttaataa ctgggataca tcttgttgca tgacccaggc cccaatccag
     3721 gggcgcgacc aggacactgg ggtgtagtcc ctgagaattg ttaatttttc cggacgtcgg
     3781 caacggtaga tactatttta cgtacaaaat agttaaatta gtaagattaa accatgagaa
     3841 ccacaaagtc agtattgatt ctgttactaa tatttagtag cattgtactc tttagctgta
     3901 gctctgggcg cggtatcggg ggcagcagca agaaatgtgg ctgcggaatc acaaaaggta
     3961 tgtctggtta ttaaaatcta atagaaatga aagcttgtaa cagggatctg cttgttttgg
     4021 tgaaagatga gcacatgaat gaaaaatcga tggagcgtga attggaacaa ttgaacgacc
     4081 tgttgttcca ttttgaaacc ttcgaatcat tctgcattgc ccacgaggtg ttcgatgtca
     4141 acaggcataa gatccttcac aaaagggaca gcatccaacg caccgtgcgt cagcgcgagt
     4201 tgaaaccctt ctttttcatc tgcaacaaaa attaggaaag ctttttcatc gcctcacgca
     4261 attttcccaa catttcagga acgtcttcca atttgcaggt acccacgctg agacggtacc
     4321 acggagaggt ctttgacgct ccgaacgcat agaaaggtac aacggccagt tttgcttcgt
     4381 cgagaagata agaagtgacg gccgcctgat cctgcaggat ccttccctcg tttgttttct
     4441 gacccgtcag atcgatcttg atcgtcaggt aaatggcggc ttcgggagtt agaacgtcca
     4501 ccttgaaccc ttcttttttc aacgccacga atccatcgtg tatgcgatgc agacgttccg
     4561 ataccgccgc gcgaaaacct ttcagataga cgtcgatcgc gcctttttgc aacaggtagg
     4621 cggcagtcgc tttctgctcc gccatcggcg cccaggaccc gatatgcgtc atgatggcct
     4681 tcatcttccc gatgatggcg ctcggtccga aagaccagcc tacccgcacc c
//
LOCUS       DASYSV010000378         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2506401, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000378 DASYSV010000000
VERSION     DASYSV010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2506401"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VGQ51_11580"
     CDS             <1..284
                     /locus_tag="VGQ51_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037259.1"
                     /translation="PGYVYYDYPAWYGHFRDRVYFEHYRPIFERDHRAYFNGGRFDHS
                     RFNNDRGYSRGFDNGRRFDNGRGYGRGSDHNRGNERGSGHDRGFHRGRF"
     gene            363..671
                     /locus_tag="VGQ51_11585"
     CDS             363..671
                     /locus_tag="VGQ51_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037260.1"
                     /translation="MKSKMLIIAMIVAGSLVAGSANAQVRVHVGINESAYPGYTYYTY
                     PSWHGHYRDRIYFQHYHDRFYRDHRGYFVGKRFDHDRYERESHWRDRHDRDRHDRDHH
                     "
     gene            complement(747..3191)
                     /locus_tag="VGQ51_11590"
     CDS             complement(747..3191)
                     /locus_tag="VGQ51_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HEV9037261.1"
                     /translation="MRNLIVVICALGLCLSAIAQETKPDLLKNKVLLPNGWTITPAGS
                     ALQLGDLPLNIAVSASGRLAAVTNNGQSIQTIQLIDVKGERVLDSVVIPRSWLGLKFS
                     ADEKYLYASGGNDNRILRYKVNPRPGSAGLVLDDSILLGKKWPEHISPAGIDIDDRRG
                     LLYVVTKDNNSLYIVDLKTKSVLQRLEIGGEAYTALLSHDGKQLYITCWGCDKLRILD
                     TQSRTFEDDIAVGDNPNDICLSKNGRWLFVANANDNSVSVIDTREKKVVETLNAALYA
                     NSLPGSTTNALALGSDGKTLYVANADNNCLAVFDVSHPGAGRSRGFIPVGWYPTAVRV
                     IDGKIWVANGKGFTSMANPLGPNPTQRRQEVNYRKGDEHKAPAVQYIAGLFKGTMSII
                     PEPDDQLLAQYSQQVYQNTPYAKDSELVAAGEPGNPVPMKVGEKSPIKYVFYFIKENR
                     TYDQVLGDVSDGNGDTSLVLFGQRVTPNQHALVKEFALLDNFYVNAEVSADGHNWSMG
                     AYATDYLEKTWPTSYGQRGGDYDAEGNRAIANNKGGFIWDDCQRAGVTYRSYGEFEDG
                     GKPHLECLKGHICPSFTGWDLSVPDTTRFSQWKRDFDSLSAADALPRLSVIRAPNDHT
                     AGLRRGSPTPFAYAADNDLAIGEYVEYLSKSPIWKESVVIILEDDAQNGPDHVDAHRS
                     PVYIAGGLVKRHFVDHTMYSTTSVLRTIELILGIPPMTQYDASSTSLWRCFTTTPDLT
                     AFQALPANIDLRDKNIASNEWSKKSSAIDLTKEDRVPDRLFNEILWRGIKGNDAAPPP
                     PSRAAFIRPSPDKDGD"
     gene            complement(3254..>3598)
                     /locus_tag="VGQ51_11595"
     CDS             complement(3254..>3598)
                     /locus_tag="VGQ51_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV9037262.1"
                     /translation="HRPIATLAVTGRSTSRYFLFDNDDELCGWKNVQTGQQRLARSVG
                     GEPVPKAFSGIHVIDPSIFDFITREGKFSMVDVYLDLAADHSIRGFDHSRSLFIDVGR
                     PSSAEEAARLFP"
BASE COUNT          794 a          974 c          952 g          878 t
ORIGIN      
        1 atcccggata tgtctactat gattatcccg cctggtacgg tcacttccgg gatcgcgtat
       61 acttcgaaca ctatcgtccg atcttcgaaa gagaccacag ggcttatttc aacggcggac
      121 gttttgacca ctcgcgtttt aacaacgacc gcggttatag ccgggggttt gacaacggtc
      181 ggaggtttga taacggtcgg ggctatggac gcggatccga ccacaaccgg ggcaacgaac
      241 gtgggtcggg acatgaccgc gggtttcacc ggggtaggtt ttaattcaat attaatcaat
      301 ctgttataca aaaagggtcg tgtggccctt tttttgcatc ctacttataa aactcgctat
      361 ctatgaaaag taaaatgtta atcatcgcta tgatcgttgc ggggtcgctg gtggcgggat
      421 ccgctaacgc gcaggtacgt gtacatgtcg gcattaatga atcggcctat ccgggatata
      481 cctattatac ttatccctcc tggcacggac attaccgtga tcgtatttat ttccagcatt
      541 atcacgaccg gttctaccgg gaccacaggg ggtattttgt aggaaaacgt ttcgatcacg
      601 atcgttatga acgcgaatca cattggcgtg accgccatga tcgtgaccgt catgatcgtg
      661 accatcatta gaaccatcgg ccaacgctta cagcaaaaag gcgcggaaag atccgcgcct
      721 tttttttaat ttccgactac gccatttcaa tctccatcct tatccggcga agggcggatg
      781 aatgcggccc ggcttggcgg cggcggcgct gcgtcattcc cttttattcc tctccacaat
      841 atctcgttga agagccggtc ggggacgcgg tcttcttttg tcagatcgat agcggatgat
      901 ttttttgacc attcattcga ggcgatgttc ttatcccgca gatcgatatt cgcgggcaag
      961 gcctgaaagg cggtcagatc gggcgttgtg gtgaaacatc tccacaggct ggtagaagac
     1021 gcatcatact gcgtcatcgg tggtatacct aagataagct cgatagtgcg caatacggaa
     1081 gtcgtggaat acatcgtatg atccacgaag tgacgtttta ccaaacctcc ggcgatatat
     1141 accgggcttc gatgggcatc gacgtgatcg ggaccgttct gcgcatcgtc ttcgagtatg
     1201 attacgacgc tctctttcca gatgggactc ttgctcaggt attctacata ctctccaatg
     1261 gcaaggtcgt tgtcggccgc gtaagcaaac ggagtcggcg aaccgcgacg caacccggcc
     1321 gtatggtcat tgggtgcgcg aatgacactg agcctcggaa gggcgtccgc cgcagacagc
     1381 gaatcaaaat cccttttcca ctggctgaaa cgggtcgtat ccggaaccga cagatcccag
     1441 ccggtaaaag atgggcagat atgccctttc aggcactcga gatgcggttt tcctccatcc
     1501 tcgaattctc catagctgcg ataggtgacg cccgcacgct gacaatcgtc ccagatgaat
     1561 ccgcctttat tattggcgat agccctgttg ccttccgcgt cgtaatcgcc gccacgctgg
     1621 ccataggagg tgggccaggt cttctcgaga tagtccgtcg cataggcgcc catgctccag
     1681 ttgtgtccgt cggcgctgac ctcggcattg acataaaagt tatccaacag cgcgaattcc
     1741 tttaccaacg catgctggtt gggcgtcacc cgctgtccaa aaagcacgag gctggtatct
     1801 ccgttgccat cgcttacatc tcccaatacc tggtcatagg tgcggttttc cttgataaaa
     1861 taaaagacat attttatggg agacttctcg cccactttca tcggtaccgg attgcccggc
     1921 tcgccagcgg cgacaagctc gctgtctttc gcatagggcg tattctgata gacctgctgt
     1981 gaatattgcg ccagtaactg gtcatccggt tcgggaataa tactcatcgt ccccttgaac
     2041 agcccggcga tatattgaac ggccggagcc ttatgctcgt cgcctttccg gtaattgacc
     2101 tcctgccggc gctgtgtcgg attgggtccc agcggattgg ccatggaggt aaagcccttg
     2161 ccgttggcta cccatatctt gccgtcgatg acacgcacgg ccgtaggata ccagccgaca
     2221 gggatgaatc ccctggagcg acctgctccg ggatggctga catcgaatac tgccagacag
     2281 ttgttatccg cattggcgac atagagcgtt ttcccgtcac tgccgagcgc aagagcgttg
     2341 gtcgtcgatc cgggaagcga attggcataa agtgccgcat tcagcgtctc cacgaccttt
     2401 ttctcacggg tatcgataac agacacgcta ttgtcattgg cattggcgac gaaaagccaa
     2461 cggccgtttt tcgaaagaca gatatcgttg ggattgtctc cgacggcgat gtcgtcttcg
     2521 aacgtcctgc tctgcgtatc gaggatgcgc agcttgtcgc aaccccagca ggtgatatac
     2581 agctgttttc cgtcgtgcga caacagcgcg gtatacgctt ctccgccgat ttccagccgc
     2641 tgtagaacag atttggtctt cagatcgacg atatacaggg aattgttgtc tttcgtgacg
     2701 acgtacagca ggccgcgacg gtcatcgatg tcgatgcccg caggcgatat atgttccggc
     2761 cactttttgc cgagcaggat gctgtcgtcc aaaaccagtc ccgcagagcc ggggcgggga
     2821 ttgactttat acctcagtat ccggttgtca ttgcctccgg aggcatagag gtatttctca
     2881 tcggcgctga acttaagccc cagccatgac ctggggataa ccacactatc gagcacccgt
     2941 tcccctttta catcgatgag ctgaatggtt tggatactct gaccgttgtt ggtcactgcc
     3001 gcgagccggc ccgatgccga caccgctata ttcaatggaa gatctcccag ctgcagggcg
     3061 ctgcccgccg gtgtgatcgt ccagccgttg ggcaataaga ctttgttttt caggagatca
     3121 ggtttcgtct cctgtgcgat cgcactcagg cacagcccaa gggcgcaaat gacaacgatt
     3181 aagtttctca tatcgataaa attattccct tcggcgctac aaagtgcggt ggggttaaaa
     3241 attaatgaat attttacggg aacaaccgcg ccgcctcttc cgccgaggag ggtctgccaa
     3301 catcgatgaa caacgaccgg ctatggtcaa atccccgaat gctatggtcc gcggccaggt
     3361 cgagatagac atccaccatg gaaaatttcc cttcgcgggt aataaaatcg aagatcgaag
     3421 ggtcgatgac atggataccg ctgaaggctt tgggtacggg ctctcctccc accgaccgcg
     3481 ccagtcgttg ctggcccgtc tgaacgttct tccagccgca cagctcatca tcgttgtcga
     3541 acaaaaaata gcgcgaggtg ctacggccgg tgacagccag tgtcgcgatc ggccggtg
//
LOCUS       DASYSV010000379         5027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1481474, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000379 DASYSV010000000
VERSION     DASYSV010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481474"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VGQ51_11600"
     CDS             <1..1455
                     /locus_tag="VGQ51_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9037263.1"
                     /translation="TPTSVRNSVQSVYTKCIFPDLQTAVSNLPMTARLTGTVTKPVAE
                     LLLAKAYLTYAWWLKNPASIPTYPDSGRTDPAGHDASYYFAQAYTTALAAIENPGPYG
                     LQPTFYDVNNGPNDRNNECMLYADHTQASQQYNAASLTYSNGGAPDNFSCWMQTWNYP
                     VITSSKSSSSWANVNSVQRISNQGYGRPYIRMAPCIGVFTNTFADKTNDSRYDGTFQT
                     VYRADFVEAGLGNGTFYNANLMPISEGSPVLTFLNDEPVGTIDYSNSAYKSSVGVGVL
                     PGRSDFVVDPQGISRIVYPGLWKISAYRTDNGGGLGNPNAASTRPWNILKFSELFFIA
                     AEAAVEGAPTSAVTGTYANDGTAYGLINIIRKRAGHWKFSNANNTALVQDNSAAMVAA
                     TPATIDINYILAERSREYYGEGYRWFDLVRTGQWANLSASYQICGKNYGDHTPVTYTR
                     TITNNLWLRPIPQGQLDAMQVSAAVKAAYQNPGY"
     gene            1536..2996
                     /locus_tag="VGQ51_11605"
     CDS             1536..2996
                     /locus_tag="VGQ51_11605"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEV9037264.1"
                     /translation="MKCIDRWMFWALTLLTGPSGIAQVRVPRMVGDGMVLQRDMPIRI
                     WGFASPGEKVTVRFDGETASGVTGDDGKWMAVIAPRKAGGPYTMDIDGINHIWVKNIM
                     VGEVWFCTGQYVLQLPLDKVKDKYAGPIAHAGDVPVRRFVIHEHYDYKGPRNNVSGGR
                     WETADGSNVGGFSALEYLFALQVYDRYRVPVGLIDACVADAPAEAWLSPDALRFFPAE
                     ASAANRYADSVFPEGGGPADRMAPGGLFNGMIAPAATYTVRGILSYHGEADVARANEY
                     AEVFPILIGDWRRHWSEGQLPVLYVQLEGRGPVTQNPQESAWAVVRDAQREALRLPAT
                     GMAVAGDLNPSGGPYASDLDLDELAKRLFLSAESVAYGKKNFIYTGPVFDNMKVHGDK
                     VHVMFSDAGSDLIVKGGGELKGFTIAGEDNRFLTARAVIDGKKLIVWSDSVVKPVAVR
                     YGWADNPYGANLYNRDILFKNGLPAPTFSGRAGGKK"
     gene            3063..>5027
                     /locus_tag="VGQ51_11610"
     CDS             3063..>5027
                     /locus_tag="VGQ51_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEV9037265.1"
                     /translation="MKKTFFLTTIVCIVASCARSQAWLDPSLPIDTRVDALIRQMTPE
                     EKVYQMMNSAPAIPRLHVPEYDWWNEALHGVARSGVATVFPQPIGMAATFDDSLVHQV
                     ATDISDEARAMYNAAVKKNYVMRFGGLTFWTPNINIFRDPRWGRGQETYGEDPYLTAR
                     MGVALVRGLQGDDPHYLKVAACAKHFAVHSGPERLRHTFDAVVSQKDLWETYLPAFHA
                     LVNAKVEAVMCAYNRTDGEACCANTYLLDDVLRKQWGFKGHIVSDCDALADIYEGHKL
                     VSSKTGAAALALQRGVNLNCGDTYLSLIDAQKQGLVTEKEIDSSLAVLLRTRFKLGLF
                     DPAGSNPYDRIPENVINSPEHRLLARKVAEKSMVLLKNDGVLPLSNGLKKYYITGPNA
                     AAVDVLLGNYYGVNDRLVTILEGLARQAQPGSQMTYRQGVLLDRPNVNPIDWASGEAQ
                     TADATIAVLGISGLIEGEEGESLASPYFGDRGDYNLPAAQIDYLRKLKKGNPHPVIAV
                     ITGGSPMNLAEVQALADAVLLVWYPGEEGGNAVADILFGKVSPSGKLPVTFPKSYDQL
                     PPYTDYSMKGRTYRYMTAEPLYPFGFGLSYTTFAYSDLQVSKARIRKDEPLNVDVTLT
                     NTGKYASDEVAQLYLTHEGMPNEPLFALKGF"
BASE COUNT         1109 a         1289 c         1554 g         1075 t
ORIGIN      
        1 acgccgactt ccgttcgcaa tagcgtccag agcgtgtaca ccaagtgtat cttccccgac
       61 ctgcagacgg ccgtgagcaa cctgccgatg accgcccggt tgaccgggac cgttaccaag
      121 ccggtggccg agctcctttt agccaaggcg tacctgacct atgcctggtg gctgaagaat
      181 cctgccagca tacctaccta cccggattcc ggacgcacgg atcccgcagg tcatgatgcg
      241 agttattatt ttgcgcaggc ctacacaacg gcgttagccg ctatcgagaa ccccggtccg
      301 tatggattgc agccgacttt ttacgatgtc aacaatgggc cgaatgaccg caacaacgaa
      361 tgtatgctgt atgccgacca tacacaggcc agccagcaat acaatgccgc cagcctcacc
      421 tatagcaatg gcggcgcacc cgacaatttc tcctgctgga tgcagacctg gaattacccg
      481 gtcatcacga gcagcaaatc cagctcctcc tgggcaaacg tgaattccgt acagaggatc
      541 tcgaaccagg ggtacggccg tccgtatatc cgcatggcgc cttgtatcgg cgtattcacc
      601 aatacgtttg ccgacaagac gaacgactcc cggtatgacg gtacgttcca aacggtgtac
      661 cgggcagatt ttgtagaggc cggtttgggc aatggtacgt tctacaatgc caacctgatg
      721 cccatttcgg agggaagtcc ggtgcttacc ttcctgaacg atgagccggt cgggaccatc
      781 gattattcca actcggcata taaaagcagt gtcggcgtag gcgtcctgcc gggcaggtcg
      841 gatttcgtgg tggatccgca gggtatcagc cggatcgtgt acccggggct ttggaagatc
      901 agcgcctatc gtacggataa cggaggcggc ctgggcaacc cgaacgcggc cagcacccgg
      961 ccgtggaata tcctcaagtt ttccgagttg ttctttatcg ccgcggaagc tgcggtagag
     1021 ggcgccccga cctcggccgt caccggcaca tacgccaatg acggtaccgc ctatggattg
     1081 atcaacatca tccgcaaacg ggccggccat tggaaattca gcaatgccaa taacacagcg
     1141 ctggtgcagg ataacagtgc ggcgatggtt gcggcgacac cggcgacgat cgacatcaat
     1201 tacattttgg cggagcgttc gcgcgaatat tatggtgagg gttaccgctg gttcgacctg
     1261 gtccgtaccg gtcagtgggc caatctgtca gcgagttacc agatctgcgg caagaattat
     1321 ggcgatcata ctcccgtaac gtatacgcgt acgatcacga acaatctctg gctgcgtcct
     1381 atcccgcaag ggcaactgga tgccatgcag gtgagcgccg cggtgaaggc ggcgtatcag
     1441 aatcccgggt attaagattt ttgtacaagt atagtttagc aatccttccc taaaccaaaa
     1501 gccggatgta tatccggctt tttttatatt gcgatatgaa atgtatcgat cgctggatgt
     1561 tttgggcctt aacgctgttg acagggcctt cgggtatcgc acaggtgagg gtgccgcgga
     1621 tggtcgggga cggcatggta ttgcagcgtg acatgcctat ccgtatctgg ggttttgcat
     1681 cgccgggcga gaaggtgacg gtgaggttcg acggagagac ggccagcgga gtcaccgggg
     1741 atgacgggaa atggatggcg gttatcgcgc ccaggaaagc agggggtccg tataccatgg
     1801 atatcgacgg catcaatcat atctgggtga agaatatcat ggtgggcgag gtttggttct
     1861 gcacggggca gtatgtgctg cagttgccgc tagacaaggt aaaagataag tatgccgggc
     1921 cgatcgcgca cgcgggcgac gttcctgtgc gtcgattcgt catccatgaa cattacgatt
     1981 acaagggtcc caggaacaat gtatccggcg gacgctggga gacggctgac gggtcgaatg
     2041 taggcggctt ttcggcgctg gaatatcttt ttgccctgca ggtgtatgac cggtatcgtg
     2101 tcccggtggg gctcatcgat gcgtgtgttg cggatgcgcc tgcggaagcg tggctgagcc
     2161 ccgatgcgct gcggtttttt cccgccgaag cttctgcggc caaccgttat gcggacagcg
     2221 ttttccctga gggtgggggg ccggccgacc ggatggcgcc cggcggatta ttcaacggca
     2281 tgatcgcgcc ggccgcaacg tatactgtgc gtggcatcct ttcgtatcac ggagaagccg
     2341 acgtagccag ggcgaatgag tatgcggagg tgttccctat cctgatcggg gattggcgaa
     2401 gacattggag cgagggacag cttcccgtcc tgtatgttca gttggagggg cgtggcccgg
     2461 tgacgcaaaa tccgcaggaa agcgcatggg ccgtggtgcg cgacgctcag cgcgaagcgt
     2521 tgaggctgcc ggcaaccggg atggcggtcg ccggagatct gaatccgtcc ggtgggccgt
     2581 acgcttccga cctcgacctg gacgagctgg ccaagcggct tttcctgtcg gcggagagcg
     2641 tagcttacgg caaaaagaat ttcatttata cgggaccggt attcgataac atgaaggtgc
     2701 atggagataa ggtccatgtc atgttctcgg atgcgggcag cgatctcatc gtcaagggag
     2761 gcggtgaatt gaagggtttt accatcgccg gcgaggacaa ccggttcctg acggcgagag
     2821 ccgtgatcga cgggaagaag ttgatcgtgt ggagtgattc ggtcgtgaaa ccggtggccg
     2881 taagatacgg gtgggccgac aatccttatg gggccaacct gtacaacagg gatatacttt
     2941 tcaagaatgg attgccggcc cctacttttt ccggacgggc cggtgggaaa aagtagaaaa
     3001 tgtaatcggt tgcattatct gctcattttt acctacatta gccgaaaatc agcagcattg
     3061 ccatgaaaaa gacttttttt cttaccacga tcgtttgcat cgtcgcttcc tgtgcgcgga
     3121 gccaggcctg gctggaccca tcattaccca tcgacacccg tgtggatgcc cttatccggc
     3181 aaatgacgcc ggaggagaag gtctaccaga tgatgaacag cgccccggct ataccacggt
     3241 tacatgtacc cgaatatgat tggtggaacg aagcgctgca tggcgtggcc cgctcgggcg
     3301 tcgccaccgt tttcccgcag ccgatcggca tggccgccac cttcgacgac agcttggtgc
     3361 accaggtggc cacggatatc tccgatgagg ccagggcgat gtacaacgcc gcggtgaaaa
     3421 agaattatgt gatgcggttc ggcggtctga ctttctggac tccgaacatc aatatcttcc
     3481 gcgacccgag atggggcagg gggcaggaga cctatggtga ggacccgtat ctgaccgcga
     3541 ggatgggcgt agcgctggtg aggggtctgc agggcgatga cccgcattat ctgaaagtgg
     3601 cggcctgcgc gaaacacttt gccgtccata gcggaccgga gcggctgcgt catactttcg
     3661 acgcggtagt ctctcagaag gacttgtggg agacttatct gcccgccttt catgcgctgg
     3721 tcaatgcgaa ggtagaggcg gtgatgtgcg cctataaccg aacggatggc gaagcctgct
     3781 gtgccaatac ctatctgctg gatgatgtgc tgcgaaaaca gtggggattc aagggacata
     3841 tcgtatcgga ctgcgacgcg ctcgccgata tctatgaagg gcataaactg gtgtcgagta
     3901 aaacgggagc agcggcatta gcgttgcagc gcggagtaaa cctgaattgc ggggacacgt
     3961 atctgtcgct gatagatgct cagaaacagg gtctggtgac agagaaggag atcgacagca
     4021 gcctggcggt attgcttcgt accagattca agctggggtt attcgacccc gcgggctcga
     4081 acccctacga tcgcataccc gagaacgtta ttaacagccc cgagcatcgg ctgctggcgc
     4141 ggaaggtggc ggaaaaatcc atggtattgc tgaagaatga cggtgtgctg ccgttgtcaa
     4201 acggacttaa aaaatattat atcacgggtc cgaacgccgc cgccgtcgac gtgctgttgg
     4261 gcaactatta cggcgtaaat gaccggctcg taacgatact ggaggggctt gccaggcagg
     4321 cgcagccggg aagtcagatg acctacaggc agggtgtact gctggatcgt ccgaacgtga
     4381 atcccatcga ctgggcttcc ggtgaagcgc agacggcgga cgcgacgatc gctgtgctgg
     4441 gcatctcggg actcattgaa ggagaggaag gggaatcgct ggcctcgcca tattttggcg
     4501 acaggggtga ttacaatctc ccggccgcgc agatcgatta tctccgaaaa ctgaagaagg
     4561 gtaatccgca tcccgtcatt gccgtcatca ccgggggcag cccgatgaac ctggcggaag
     4621 tgcaggcact ggccgatgca gtgctgctgg tatggtatcc cggggaagag ggggggaacg
     4681 ccgtggcgga cattttgttt ggaaaagttt cgccgtcggg gaaactgcct gtaaccttcc
     4741 ccaaatctta tgatcaatta ccgccttaca ccgattattc tatgaagggc aggacgtacc
     4801 ggtatatgac ggcggagcct ttgtatccct tcggattcgg gttgagctat accacctttg
     4861 cgtacagtga tctgcaggtt tcgaaagcac ggatcaggaa ggatgagccg ttgaatgtgg
     4921 acgtgacgct gaccaatacg ggaaaatatg cgtcggatga agtggcgcag ctatacctga
     4981 cacatgaggg gatgcccaat gagcctttat ttgcattaaa aggattt
//
LOCUS       DASYSV010000380        14058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_586950, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000380 DASYSV010000000
VERSION     DASYSV010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 14058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14058
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586950"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VGQ51_11615"
     CDS             <1..299
                     /locus_tag="VGQ51_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9037266.1"
                     /translation="QRVKEIGIRKVLGASIASIGALLSRDFLRPVLLAVLIACPLAWW
                     IMHRWLQDFAYRTSLSWWIFVVAGFGLLGIALVTVLMRSLKAGRANPVDNLRTE"
     gene            346..1461
                     /locus_tag="VGQ51_11620"
     CDS             346..1461
                     /locus_tag="VGQ51_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEV9037267.1"
                     /translation="MFIFFRRTTPLLMIALVICLMSLGQEAPQSTLHPGFDGKEYREL
                     LDYAFHGRLTSDRSAGAGQPYTLQYQSPEVGMRNRWDLWVRDDKQIAVISIRGTVNDR
                     LSWLENFYAAMVPATGSVQLSDSVKFDYRLSADDKAMVHAGWLIGLAYLAPTMVEQLR
                     RVYAGGIHSILIFGHSQGGALAFLTRSYFYYLQQEGQLPQDIVFKTYCSAAPKPGNTF
                     YAYDFDFITRGGWAFTVVNAADWVPETPFTVQTLTDLNPVNPFTHVDKALQKQAFFVR
                     LYIRGKYNKLGRHTRKAQKAMQEILGDKVFKAIKRSLPGLREPFYAGGSNYQRAGVPI
                     VLEPDPAYYQLFPNDPDKIFLHHSFDAYSWLATKYYK"
     gene            1617..1919
                     /locus_tag="VGQ51_11625"
     CDS             1617..1919
                     /locus_tag="VGQ51_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV9037268.1"
                     /translation="MNMQKVMIGALSGLVAGVAIGILVAPAEGKETRQRITDTADSLK
                     RKLRQLRGVTADELDELKDIFERETEGLRDDVRTRVLSLIRAAKERGNHIKEQALS"
     gene            2038..3057
                     /locus_tag="VGQ51_11630"
     CDS             2038..3057
                     /locus_tag="VGQ51_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEV9037269.1"
                     /translation="MPIDLRSDTVTRPTPAMLDAMMSAKVGDDVFGEDPTINILEAMA
                     ARMFGMEAALYCPSGTMSNQIAIKCHTQPGDEVVCEKSAHVYVYEGGGIAFNSGSQAR
                     TLEGNLGRITAAQVSMVINPDDVHRARTSLVTLENTCNRGGGSCYDLEEIGKIRQVCS
                     DNQLKLHLDGARLFNALVATGQSAPAYGRLFDSISVCLNKGLGCPIGSILIGPAAFIR
                     HARRVRKVFGGGMRQAGFMAAAGVYALENHIDRLAHDHAHARRIASALEKKKFVRSIM
                     PVETNIIIFEVEGDGCAKQLAQRLREKDILAIPISANQIRFVTHLDISAEMVDKVVSV
                     IEQLN"
     gene            3069..4697
                     /gene="pgi"
                     /locus_tag="VGQ51_11635"
     CDS             3069..4697
                     /gene="pgi"
                     /locus_tag="VGQ51_11635"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV9037270.1"
                     /translation="MFPKVTPKETKAWQQLQKHHDQEMKDVHMRSLFAADSQRFDKYT
                     LRLDDILFDYSKNIFSEKTLTQLRQLADECKVKDAIEAMFTGEKINATENRSVLHTAL
                     RNLSGTPVCSDGKDVMPDVLRVLAQMKDFSGRIHSGEWKGYTGKKIRYIVNIGIGGSD
                     LGPVMVTEALRPYWKEGIQAFFVSNVDATHIVETLKKVTPEETLFLIASKTFTTQETM
                     TNAHSARDWFLKAAKDEKHVAKHFAALSTNEKEVVRFGIDKANMFEFWDWVGGRYSLW
                     SAIGLSIVLTIGYDNFEQLLQGAHAADNHFRTTPFEKNIPVTMALIGLWYTNFFGSQT
                     EAILPYDQYMHRFAAYFQQGNMESNGKSVDRNGKPVDWSTGPVVWGEPGTNGQHAFYQ
                     LIHQGTVLIPCDFIAPAISHNPLGDHHVKLLSNFFAQTEALMNGKTVAGLEAEKVPPA
                     LIPYKGFRGNRPTNSFLIREITPYSLGELIALYEHKIFVQGVIWDIYTFDQWGVELGK
                     QLAGKILPELVGSGEVKSHDSSTNGLINAFKKMR"
     gene            5180..7612
                     /locus_tag="VGQ51_11640"
     CDS             5180..7612
                     /locus_tag="VGQ51_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037271.1"
                     /translation="MVMFKSYWTIAWRTLVRNRIYTLVNVAGLALGICACLVIWVVVH
                     YEFSFDRNHPGGARIFRVNSYEQYMKDEPEQLVPEVVTQLPEMIRNQITGVETLAAYH
                     ELHNWTATVAGASKGQKTDYTAQPIVAGPEYFTIMTYDWLAGDARTALSTPFGVVLTE
                     KRARQFFGANTPPAAIIGREVVYNDSLHVHVTGIVRDWSGHTDFPFTEFISYSTIDHS
                     WLQYTLGVDANSKRLAMCSVLMKLAAKADAAKVNAAIASMYKRSWPGGIIPRVEMQPL
                     SDVHFTPRQDETAFRTSQLSTLYGLLCIALFILVLAIVNYVNLATAQSLTREKEISIR
                     KVMGSGRRSLMIQLLTETFLLTAVAGVIAVLSVRPVLGAFRQLVPETIQFDPLAPANW
                     LFLLAITIATTLLAGIYPARMLSAHSPVIGLKGAGAPRGGGKWWLRRGLIVFQFTVSL
                     LFIICTMVIGRQIGFMLSKDLGFRTDAIILFSTDEQRDSVSKVKVMEEEIRQLPGVAE
                     ISRGNIPPMGPDRAMTGIRYPVTNPDHLSVEFVGADEHYIPLYGIRLLAGRNLFPSDT
                     IKEAVINETLSRRLGFRSPEEAVGKLVYTWGKNVPIVGVVADFHEFSYKEAIEPLMLV
                     GRASTDLQLRMDTKGRSTAEVRIILGRIERQWHTVYPHKVFNYSFFDEWIAQLYRREQ
                     TMEWLMNIATAITVFISCIGLFGLTLFTTERRTREIGIRKVLGAGVKDILTLLGKDFA
                     RLVMIALVIASAGGWYFMHRWLQDYVYRAKLGPDVFLLAGGFLLVVTLLTVGVQSLRA
                     ALVNPVRSLSSE"
     gene            7718..8779
                     /locus_tag="VGQ51_11645"
     CDS             7718..8779
                     /locus_tag="VGQ51_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037272.1"
                     /translation="MACVAGICGLVVFSACNNGRSEAGKKADSAAVKSGNKDTVVAPG
                     EAAASVPLAPLDTTLYNKLVVHLANGDSSGRWPVKTAYPNAGALLPFNRIVAYYGNLY
                     SKNMGVLGEFPPNEMLAKLKAECKKWQEADTTVHVLPALHYIAVTAQGSPGKDGKYRA
                     RMPFKHIDSILHMAARINAIVFLDIQVGFSNVQSEVPMLEKYLKMPNVHLGIDPEFAM
                     SKSGKKPGTVIGTLDAADANWCISYLTRLVRENNIPPKILMFHRFTRNMLTNARQIHP
                     TPQCQFILDMDGWGPVSLKTDSYKSYVADFPIEFTGFKLFYKNDTRTPRGVKLKETQL
                     MTPKQVLAQKPSPIYIQYQ"
     gene            complement(8783..9250)
                     /locus_tag="VGQ51_11650"
     CDS             complement(8783..9250)
                     /locus_tag="VGQ51_11650"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV9037273.1"
                     /translation="MTTLQQLRNWSATHHPRWLVIVRIGLGLFLLAKGINFIRDSTLL
                     DRLLYGGSSMAENEPHWLPLIITWANLLGGFMIMIGLWTRLVCLLQLPILIGAIIFIN
                     AQRGGFAPESELGLAILTLLLAIFFLIEGSGPLSLDAYFDRNKGRGSQGSNLP"
     gene            complement(9297..9716)
                     /locus_tag="VGQ51_11655"
     CDS             complement(9297..9716)
                     /locus_tag="VGQ51_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9037274.1"
                     /translation="MSIRPATIGDGAAISRLLTQLDYPGTEEFVEARLATMLQDPAEV
                     LLVWDDPGVKGFVSLHFIPMIGVGKDFAQISYLIVDDDTRSEGIGRRLEETVTRIARE
                     RGCDRIIVHCDSRRIRAHAFYLRQGFEESPKYLIKRL"
     gene            complement(9723..11549)
                     /locus_tag="VGQ51_11660"
     CDS             complement(9723..11549)
                     /locus_tag="VGQ51_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV9037275.1"
                     /translation="MYLNRLCFVLVLLFGWQLISAQRISYIVSFPSLAHHEARIELVV
                     SDIPNRTAVFRMSRSSPGRYATHEFGKNVYDISAFDQAGKPLTINRTDGDVWEVSRHS
                     GYIRVQYTLYGNYADGTYAGLDPDGVHLNMPAAFLWMKGIDNVPITIHFNVPPEWHWT
                     VATQLQPTGDSLTFAARNFQYFMDCPTKIGPLRYRRWEVRNPDGKAYAFRIALDGAGD
                     DSTFTAFSEKVQKIVRQEQAVFGETPAYDYGTYTFLASINPYVHGDGMEHRNSTMISI
                     PVAFHGDDRLLGVFAHEFFHCWNVKRIRPRSLEPFNFEKSDMSRELWFAEGFTQYYGE
                     LMVERAGFARDTDFVKHTLAGLINAKMNTPGARLYSPVEASEEAVFTDAGVAVDKTNF
                     PNIFTTYYFYGGAIAVALDLELRSRFGKTLDGYMTAAWKKFGKPEIPYNVEGLEEVLS
                     SYTGDKKFADAWFSRYINGHQPIDYSALLAPAGFLLMPAEGNKAWIGNTRSFTEKEGL
                     IIGTGTVRGTPLYDAGLDVDDKILSIDNLDVRTIADLNNVLSHLHPGASVDIRYLHRN
                     EERKGTIRPIENPAMSVVTFEQAGRPLSPAILQFRKSWLSAK"
     gene            complement(11589..11951)
                     /locus_tag="VGQ51_11665"
     CDS             complement(11589..11951)
                     /locus_tag="VGQ51_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037276.1"
                     /translation="MNKDWLKILSESNKDIDNQQLMDYLSGKLSGEALHELERSMADN
                     PLLNDAVEGLQQLKNKKNMQTYVDELNTAMLKSLDKKKNRRLRRRLRDTPWTYFAIIL
                     VLALCIIGYFLVRKMLVG"
     gene            complement(11948..12529)
                     /locus_tag="VGQ51_11670"
     CDS             complement(11948..12529)
                     /locus_tag="VGQ51_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9037277.1"
                     /translation="MKDPLHHMTDQELLSRYYEEHNNEWLGALLQRYTLLLFGVCMKY
                     LKEEEEARDCVQQIFIKVITELPKYKVEYFKSWIYTIARNHCLMKIRDRHGHKTGLSD
                     SLLAAWDEEGGDSLHLEKERLLEWMEQALNELVKEQKLCVTLFYLDKRSYQEIATSTG
                     FTLMQVKSYIQNGKRNLRLLIEKKAREGQAHRK"
     gene            12600..13379
                     /locus_tag="VGQ51_11675"
     CDS             12600..13379
                     /locus_tag="VGQ51_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEV9037278.1"
                     /translation="MIRAICCVPVSPLRAEPSHRTEMVSQLIFGECCEVLEQTKDQWS
                     RVRVRYDGYEGWCTSPHLTEIDEKEFAAERTPLTPEWVASLEYNGHPMMVPMGSHLTA
                     MKNGRAQWRKNRVHYKGKTWEPGERAGDAKSIRQLTLKFLNVPYLWGGKSIFGIDCSG
                     YAQTVYKFLGVPILRDAHQQAGQGDPVGFLEETKTGDLAFFDNEEGRITHVGILLNSN
                     EIIHASGKVRIDKIDNMGIVQSDTGLRTHHLRLIKRMLPED"
     gene            complement(13460..14005)
                     /locus_tag="VGQ51_11680"
     CDS             complement(13460..14005)
                     /locus_tag="VGQ51_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEV9037279.1"
                     /translation="MITVSHPWHGVPVGDGSPRVVNAIIEIPQGSRAKYEIDKDSGLL
                     KLDRVIYSSFYYPVNYGFIPQTYGDDKDPLDILVITSLPVQALCLVQAKVMGVMQMVD
                     SGDADDKIIAVAANDPSVNHYNNLEELPRHFFEELRHFFEEYKKLENKTVVVEEFGDK
                     TTALKVIEDAVRLYDETFRKK"
BASE COUNT         3164 a         3661 c         4033 g         3200 t
ORIGIN      
        1 gccagcgggt gaaggagatc ggcatccgca aggtattggg cgcatccatt gcatccatcg
       61 gggcgttgtt gtcgcgggat tttcttcgac ccgtattgct ggccgtgctc atcgcctgtc
      121 cgttggcgtg gtggatcatg caccggtggc tacaggattt tgcctaccga acgtcattgt
      181 cgtggtggat attcgtcgtg gcagggtttg ggctgctggg cattgcgctg gtcactgtgt
      241 tgatgcgaag cctgaaggcc ggcagggcca atcccgtcga taaccttcgg acagagtaga
      301 ccgttacggc cggcacgggg ggcgcggaat tgcgtacatt cgggcatgtt tatctttttt
      361 cgccggacca caccgttact gatgatcgcc ctggtcatct gccttatgtc gttgggacag
      421 gaggcgccgc aatcgacgct ccatcccggc ttcgatggaa aggaataccg agaattgctg
      481 gactatgcct ttcacgggcg tctgacatcc gacaggtcag ctggcgccgg gcagccgtat
      541 acgttacaat accaaagccc cgaagttggc atgcgcaacc gctgggatct atgggtgcgc
      601 gacgacaaac agatagcggt gatctcgatc cgcggtactg tcaacgacag gctaagctgg
      661 ctggagaatt tttatgcggc catggtgccg gctacgggat ctgtgcagct aagcgatagc
      721 gtgaaattcg attaccggtt gtctgcggac gataaggcaa tggttcatgc gggatggctg
      781 atcgggctgg cctacctcgc gcccacgatg gtggagcagc tgcgccgggt ctatgccggt
      841 ggcatccatt ccatccttat cttcggacat agccagggcg gtgcgctggc atttcttacc
      901 cgcagttatt tttattatct gcaacaagag ggacagctgc cgcaggacat cgtcttcaag
      961 acctattgca gcgccgcgcc gaagccgggc aatacctttt atgcctacga tttcgatttc
     1021 atcacgcgtg gaggctgggc ctttacggtg gtcaatgcgg cggactgggt gccggagacc
     1081 cccttcaccg ttcaaaccct gaccgatctc aacccggtga atccttttac acatgtcgac
     1141 aaagccttgc aaaaacaggc ttttttcgtc aggctttaca tcaggggcaa atacaataag
     1201 ctcgggcgtc acacgcgaaa ggcgcaaaag gccatgcagg agatattagg cgacaaggtt
     1261 tttaaagcga taaagcgatc tctgccggga cttcgcgaac cattctatgc cggcggcagc
     1321 aattatcaac gggccggtgt gcctatcgta ctggagccgg atccggctta ttatcagctg
     1381 tttcccaatg accccgacaa gatctttctt caccatagct tcgatgccta tagctggctg
     1441 gcgaccaaat attacaaata agcaacttga tccgcgttcg cgcctccggg tggggataaa
     1501 ttttcccagt cggtggtgag tattctctag cgttccttta acttttttcg gcattgttct
     1561 tggaaggttc aaaatagccc gtgttcaaaa agatttgttc acttaaaatt tatcgtatga
     1621 acatgcagaa agttatgatc ggcgccttat caggccttgt cgcaggtgtt gcgatcggca
     1681 tccttgtcgc tccggcagag gggaaagaaa cccgtcagcg gattaccgat acggcagatt
     1741 ctttgaaacg taagctcaga caattgagag gcgtcacagc ggatgaattg gacgagttga
     1801 aggatatatt cgaacgggaa acggaaggtt tgcgcgatga tgtgcgcacc cgcgtgttgt
     1861 cattgataag ggctgccaaa gagagaggga accacatcaa ggaacaggcc ttgtcttagg
     1921 acatcggacc tgggatgcaa gataaagaag ggagtcatac tggcttcctt ttttgttggt
     1981 ggcgctgtta gtcgggtctg ctcttttccc cttaaattgc ggtcctaaac tatctgtatg
     2041 cccatcgatc ttcgttcgga taccgttacg cgacctaccc ccgccatgct ggatgccatg
     2101 atgagcgcaa aagtcggtga cgatgttttt ggagaggacc ctacgatcaa tattttggaa
     2161 gccatggccg cgcggatgtt cgggatggaa gcggcgctct attgtcccag cggaacgatg
     2221 tccaaccaga tagcgatcaa atgtcacaca caacccggcg atgaggtcgt ttgcgagaaa
     2281 agcgcgcatg tttatgtgta tgagggaggc ggcatcgcct tcaattcggg cagccaggcg
     2341 cgcacgctgg aaggcaatct tggccgaatc acggccgcac aggtatcgat ggtcatcaat
     2401 cccgacgatg tacatcgggc ccgaacctca ctcgtcacgc tggagaacac ctgtaatagg
     2461 ggagggggca gctgttatga cctggaagag atcgggaaga tccgccaggt atgcagcgac
     2521 aatcagctga agctgcatct cgacggcgcc cgtctgttca acgcgctggt agccaccggc
     2581 cagtccgccc cggcctatgg ccgtcttttc gatagcattt ccgtctgtct gaacaagggg
     2641 ctgggctgtc ccatcggcag catcctgata ggtccggcgg cttttatccg ccatgcgcga
     2701 cgcgtccgaa aagtattcgg cgggggaatg cgtcaggccg gcttcatggc tgccgccggc
     2761 gtttatgcgc tggaaaatca tatcgaccgg ttggctcacg atcatgcaca tgcgcgccgt
     2821 atcgcctcgg cattggagaa aaagaagttc gtccgctcga tcatgccggt ggagaccaat
     2881 atcatcatct tcgaagtgga aggagacggc tgcgccaaac agctggcgca gcgtctgagg
     2941 gaaaaggaca tcctggccat ccccatttcc gccaaccaga tacggttcgt cacgcatctc
     3001 gacatcagcg ctgagatggt ggataaggtc gtgtcggtca ttgaacaact caattaaact
     3061 atattctcat gttcccgaaa gtcactccaa aggaaacgaa agcctggcag caactgcaaa
     3121 aacatcatga ccaggagatg aaagacgtcc acatgcgttc gctattcgcg gcggacagtc
     3181 aacggttcga caagtatacg ctccggctgg acgatatcct tttcgactat tcgaaaaaca
     3241 ttttttccga aaagacgctg acccagcttc gtcagctggc cgatgaatgc aaggtcaaag
     3301 acgccatcga agcgatgttc accggggaga agatcaatgc gacggagaac agatcggtcc
     3361 ttcacaccgc cctccgcaat ctttccggga cgcctgtctg cagcgacggg aaggatgtta
     3421 tgccggatgt gctgcgggtg ctggcacaga tgaaggattt ctcggggagg atccacagcg
     3481 gcgaatggaa gggctacacc gggaaaaaga tcagatacat cgtcaatatc ggcatcggcg
     3541 gcagcgatct gggccctgtg atggtcaccg aggcattgcg tccgtactgg aaggaaggta
     3601 tacaggcctt cttcgtgagc aatgtcgacg ccacgcatat cgtcgagacg ttgaaaaaag
     3661 taacgcccga agagacgctg ttcctcatcg cgtccaagac ctttaccacg caggagacga
     3721 tgaccaatgc gcattcggcg cgcgactggt tcctgaaggc cgccaaagac gagaagcatg
     3781 tcgccaaaca ttttgcggca ttgtccacca atgagaagga agtagtgagg ttcggtatcg
     3841 acaaggccaa tatgttcgaa ttctgggact gggtcggcgg ccgttattcg ctatggagcg
     3901 ccatcggtct ttccattgta ttgaccatcg gctatgacaa tttcgaacaa ctgctccagg
     3961 gggcgcacgc ggccgataat cacttccgga cgactccttt cgaaaaaaat attccggtga
     4021 ctatggccct gatcggtctc tggtacacca acttcttcgg ttcacagacc gaggccatat
     4081 tgccgtacga ccaatacatg caccggtttg ccgcgtattt tcagcagggc aatatggaaa
     4141 gcaacggcaa gagcgtggac cgcaacggga aaccggtaga ctggtcgacc ggtccggtag
     4201 tctggggcga gcccggcacc aatggtcagc atgcattcta ccagttgata caccagggca
     4261 ccgtcctgat cccctgcgac ttcatcgcgc ccgccatcag ccacaatccg ctgggggatc
     4321 atcatgtcaa gctcttgtcc aatttcttcg cgcagaccga ggcgctgatg aatggcaaaa
     4381 ccgttgccgg gctcgaagcc gaaaaggtgc ctccggcctt gatcccctat aaaggcttcc
     4441 gcggcaatcg ccccaccaac tcctttttga tccgcgagat cacgccatat tctctcggcg
     4501 agctgatcgc cttgtatgag cataagatat tcgtgcaggg cgtcatctgg gacatctaca
     4561 ccttcgatca atggggtgtc gagctgggta aacagctggc cgggaagatc ctgccggaat
     4621 tagtcggcag cggggaggtg aaatcccatg actcgtcgac caacgggttg atcaatgcct
     4681 tcaagaaaat gcggtaggcg gttgtgtaat ttcgcgatcc agggcatccg tgtaaaaaat
     4741 acgcgctatg aagtggtttt tttatgtttt gctccttgcc gggccactcg cggtatgcgg
     4801 acaatgcggt ctatccggcg cgatcccaca ttggtcgccc aaaaaaatat cgctggatcc
     4861 gctacgggat tttcgtcgga atttcgatcc gcgacgatgc tttgccgata cgaatatgct
     4921 gagtcagagg atggcttccg gaaggcggga tccactgaga ttcgaacctc ctgacgggat
     4981 gccttgcacg atattggatt atcaggttgg aaggatgccg gtgaaccgat cgggtaatgc
     5041 agacaaaatg cctaacccgc tgggaaacaa ggcaagcgga cacccgttgt atcattagcg
     5101 gacaagacgg tagctccgga cgggtatgcg acactgataa ccagctattt accgatccgg
     5161 catcgaacgt gtgagagaga tggtcatgtt caagagctac tggactatcg cctggcgtac
     5221 gctggtccgc aaccggatct ataccctcgt caatgtcgcc gggctggcgc tcggcatctg
     5281 cgcctgtctg gtcatctggg tcgtcgtgca ttatgagttc tcgttcgacc gcaaccatcc
     5341 cggcggagcg cgcatctttc gcgtcaactc ctatgagcaa tacatgaagg atgagccgga
     5401 gcagctggtt ccggaggttg tgactcaatt gcccgagatg atccgtaacc agatcaccgg
     5461 tgtggagacc ctggcagctt atcatgagtt gcataattgg acggcgaccg ttgccggggc
     5521 atcgaagggg caaaagaccg actacaccgc ccaaccgata gtcgccggac ccgaatattt
     5581 cacgatcatg acctatgatt ggctggcggg tgatgcgcga accgcgctgt cgactccttt
     5641 cggtgtcgtg ctcaccgaga aaagagcaag acagtttttc ggtgcaaata cgccacccgc
     5701 cgcgatcatc ggcagggaag tcgtgtataa cgacagcctg catgtccacg tcaccggcat
     5761 cgtccgtgat tggagcggac atacggattt cccttttacc gaattcatat catactccac
     5821 catcgatcac agctggctcc agtacaccct gggcgtggac gccaatagca aacggctggc
     5881 gatgtgttcg gtgctgatga agctggcggc gaaggcagat gcggcaaaag tgaatgccgc
     5941 gatcgcgtcg atgtataaga ggagctggcc ggggggaata atcccccgcg tggagatgca
     6001 gcccctgagc gacgtacatt tcacgccccg gcaggatgag acggccttcc ggacctctca
     6061 gctatccacc ctatatgggt tgttgtgtat tgccctattc atccttgtgc tggccatcgt
     6121 caactatgtc aatctcgcca cagcccagtc gctgacgagg gaaaaggaga tcagcatccg
     6181 gaaggtgatg ggcagcggcc gccggagcct gatgatccag ctgcttacgg agacctttct
     6241 gctgacggca gtcgccggag tgatcgcggt gctgtctgta agaccggtat tgggggcctt
     6301 ccggcagttg gtaccagaga ccatacaatt cgacccgctg gcgcctgcca actggctctt
     6361 cctgctggcg ataactattg ctacgaccct gctggcgggt atataccccg cacgcatgct
     6421 ttccgcgcat tcccccgtga tcggtcttaa gggcgccggc gcgccccggg gaggcgggaa
     6481 atggtggttg cggagggggt tgatcgtatt ccagttcacg gtctcgctat tgttcatcat
     6541 ctgcacgatg gtcatcggaa gacagatagg ttttatgttg tccaaagacc tcggtttcag
     6601 gaccgatgcg atcatcctct tcagcaccga tgaacaacgg gacagcgtaa gtaaggtgaa
     6661 ggtgatggag gaggagatca ggcagttgcc gggtgtcgcg gagatctcgc ggggaaatat
     6721 cccgccgatg ggacccgaca gggcgatgac gggcatcagg tacccggtta ccaaccctga
     6781 ccatttatcc gtcgaattcg tgggggccga cgaacattat atcccgctgt atgggatccg
     6841 gttgctggcc ggcaggaatc tcttccccag tgacacgatc aaagaggcgg tgataaatga
     6901 gaccttgtcc cgaaggctgg gattccggag tcctgaagag gctgtcggaa agctggtcta
     6961 tacctggggc aagaatgttc ctatcgtcgg cgtggtcgcc gatttccatg aattctccta
     7021 caaggaagct atcgagccgc tgatgctcgt tggaagggcc agcacggacc tgcagctccg
     7081 gatggacacg aagggcagga gcacggcgga agtccggatc attctcgggc gtatcgaacg
     7141 ccaatggcat acggtttatc cgcataaggt gttcaattac tcctttttcg atgaatggat
     7201 cgcgcagttg tatcgacgcg aacagaccat ggaatggctc atgaacattg ctacagccat
     7261 tactgtcttc atcagctgta tcggactttt cgggttgacg ctgttcacga ccgagcggcg
     7321 gacgcgggag atcggcatcc gcaaggtatt gggagccggc gtgaaggata tcctgacgct
     7381 attgggaaag gattttgcaa gactcgtgat gatcgcgctg gtcatcgcat cggcaggcgg
     7441 atggtatttc atgcaccgat ggttacagga ctacgtatac cgggcgaagc tcggaccgga
     7501 tgtattcctg ctcgccggag ggtttctgct ggtggtgacc ctgctgaccg tgggtgtgca
     7561 atccctccgg gcggcgttgg ttaacccggt gaggtctttg agtagcgaat aaaataaaat
     7621 cccgataggt gcgttttccg ttacctttgc gcgaatttaa aaattgcgta gaaggataaa
     7681 aatgggtaaa actaggggtt cgatcgaagt cttaaaaatg gcctgcgtgg caggcatctg
     7741 tgggttggtt gttttcagtg catgtaacaa tggccgttcg gaagccggga aaaaggcgga
     7801 cagcgccgct gttaagtcag gcaataaaga cacggtagtc gctccggggg aggccgcggc
     7861 gtctgtacct ctggctccgc tggacacgac gctctacaac aagctggtgg tacatctggc
     7921 caatggggac tcctcgggtc gctggccggt gaagaccgca tatcccaatg ccggggcctt
     7981 acttcctttc aaccgcatcg tcgcctacta tggtaacctg tattcgaaga atatgggcgt
     8041 gctgggcgaa tttccgccga atgagatgct ggccaagctg aaggctgaat gtaagaaatg
     8101 gcaggaagcc gatacgacgg tccatgtact gcctgccctc cattatatcg ccgtcacggc
     8161 ccagggatct cccggcaaag atggtaaata ccgggccagg atgccgttca aacatatcga
     8221 ttcaatactc catatggcgg cgcggatcaa tgccatcgtt ttcctggata tccaggtcgg
     8281 tttcagcaac gtgcaatcgg aagtgcccat gctggagaaa tacctcaaaa tgcccaatgt
     8341 ccatctgggt atcgatccgg aattcgctat gtccaagtcg ggtaaaaagc cgggaacggt
     8401 gatcgggaca ctggacgccg cggacgccaa ttggtgtatc agctatctca ccaggctcgt
     8461 aagggagaat aatattcctc cgaagatatt gatgttccat cgttttacca ggaacatgct
     8521 cactaacgcg cgccagatac acccgacgcc gcagtgccaa ttcatattgg acatggacgg
     8581 atggggaccc gtcagtctca agacggacag ctataagagc tatgtggccg atttcccgat
     8641 agagttcacg ggtttcaagc tattctataa gaatgacacc aggacgcccc ggggagtgaa
     8701 attgaaggag acccagctca tgacgcccaa acaggtcctg gcgcaaaaac ccagtcccat
     8761 ttatatacaa taccagtagg cgctacggta gattgctgcc ctgcgagccg cgtcctttgt
     8821 tccggtcgaa atacgcatcc agtgagaggg gcccgctgcc ttcgatgagg aaaaagatcg
     8881 ccaacagcag ggtcaggatg gccaggccaa gttcggactc cggtgcgaat cctccgcgtt
     8941 gtgcattgat gaatatgatg gcgccgatga ggatcggcag ttgaagcagg cataccagtc
     9001 ttgtccagag gccgatcatg atcatgaacc cgcccaggag gttagcccag gtgatgatca
     9061 ggggcaacca gtgcggctca ttttcggcca tgctggatcc cccatacagc aggcgatcca
     9121 gcagcgtgga atcacgaatg aaattgatcc cttttgccag caggaacagg cccaatccga
     9181 tgcgaacgat aacgagccag cgcggatgat gggtagcact ccaatttcgt agttgttgca
     9241 gcgttgtcat aacctaacat tttactacaa ttggcgttgc aattggtgtg cctgcactaa
     9301 agccttttaa tcagatactt gggagactct tcgaacccct gccggagata aaaggcgtgg
     9361 gcacgtatcc gtcgggaatc gcaatgaact ataatccggt cgcatccgcg ctcacgtgcg
     9421 atgcgcgtaa cggtctcttc cagccggcgg ccgatgcctt cgctgcgggt gtcgtcatcg
     9481 acaatgagat agctgatctg ggcgaagtcc ttgccgacac cgatcatcgg aatgaaatgc
     9541 agtgatacga atcctttgac gcccggatcg tcccaaacga gcaggacctc ggcggggtcc
     9601 tgcagcatcg tcgccagtct cgcctccacg aattcctccg taccgggata atccagctgt
     9661 gtcaggagac ggcttatggc ggctccgtca cctatcgttg cgggacgtat cgacatcgtt
     9721 gcttatttgg cggagagcca gcttttcctg aattgcagta tagcgggcga gagcggacgt
     9781 cccgcctgtt cgaaggtgac caccgacatt gcggggtttt cgatcggcct gatggtccct
     9841 ttcctttctt cgttgcgatg cagatagcgg atatccaccg aagcaccggg gtgaagatga
     9901 gagaggacat tgttcagatc ggcgatggtg cgtacatcca gattgtcgat ggacaggatc
     9961 ttgtcatcaa cgtccagtcc cgcatcgtat aggggtgttc cgcgcaccgt accggtcccg
    10021 atgatcaggc cctccttttc ggtgaagctg cgcgtgttgc cgatccaggc tttgttccct
    10081 tccgccggca tgagcaggaa gccggccggc gccagcaggg ctgaataatc tatcggctga
    10141 tgtccattga tatagcggga gaaccaggcg tccgcgaatt tcttatcacc ggtgtaggag
    10201 ctcagcacct cctccagtcc ttccacgttg taagggatct cgggtttgcc gaattttttc
    10261 caggccgccg tcatatagcc atccagcgtc ttgccaaacc ggctgcggag ctcgaggtcc
    10321 agggcgacgg cgatcgctcc tccatagaaa tagtaggtcg taaaaatatt ggggaagttg
    10381 gtcttatcca ccgccacgcc ggcatcggtg aatacggctt cttcgctggc ctcaaccggg
    10441 gaatagagac gcgcccccgg ggtgttcatt ttcgcattga tgagacccgc cagcgtatgt
    10501 ttgacgaaat cggtatcccg ggcgaagccc gcacgttcca ccatcagctc gccataatat
    10561 tgcgtaaagc cctccgcaaa ccacagctcg cggctcatgt cgctcttctc gaaattaaac
    10621 ggctctaaac tacgcggtcg tatccttttc acattccagc agtgaaagaa ctcgtgcgca
    10681 aacacgccca ggagacgatc gtcaccatgg aaagcaaccg gtatgctgat catcgtggaa
    10741 ttgcgatgtt ccatgccgtc cccatgcaca tagggattga tgctggccag gaaggtatag
    10801 gtgccatagt cgtatgcagg cgtttcaccg aataccgcct gttcctgccg gacgatcttt
    10861 tgcacttttt cgctgaaggc ggtaaacgtc gaatcgtcac ccgccccgtc gagcgcgatg
    10921 cggaacgcat atgctttacc gtcaggattg cggacctccc atctgcgata gcgcagcggt
    10981 cctattttgg tgggacagtc catgaaatac tggaaattgc gcgccgcaaa cgtcagcgaa
    11041 tcgccggtcg gctgcaattg agtggctacc gtccagtgcc attccggcgg aacgttgaaa
    11101 tgaatcgtga tcggcacgtt gtcgatgcct ttcatccaca gaaaggcggc gggcatattc
    11161 aggtggactc catcgggatc gagacccgca taggtaccat ccgcatagtt gccatagagc
    11221 gtatattgaa cccggatata accgctgtgg cgggagacct cccagacgtc gccgtccgtg
    11281 cggttgatgg tcaggggctt accggcctga tcgaaagcgc tgatgtcata gacatttttc
    11341 ccgaactcat gggtggcata tctgccggga gagctccggc tcatacggaa tacggccgtc
    11401 cggttgggga tatcgctcac gacaagctcg atccgggctt catgatgagc aagactgggg
    11461 aaactgacga tatagctgat gcgctgggcg gagatgagct gccagccgaa tagaaggacc
    11521 agcacgaaac agagccgatt gaggtacata taaacagtta ataggaggcc gaatataggg
    11581 aattaatctc agcccaccag cattttgcgg acgaggaaat aaccgatgat gcagagcgcc
    11641 agcacgagga tgatggcaaa ataggtccag ggggtatccc ggagacggcg gcgcagacgc
    11701 ctgttcttct ttttatccag gcttttgagc atggccgtat tgagctcgtc cacataggtc
    11761 tgcatattct ttttgttctt cagctgctgc aaaccttcca cggcgtcatt gaggaggggg
    11821 ttatctgcca tggagcgttc cagctcatga agggcttctc cggacaattt gccgctgaga
    11881 tagtccatca actgctggtt gtcgatgtcc ttattgcttt cggataatat tttcagccag
    11941 tccttattca ttttcgatgt gcctggccct cgcgggcctt tttttcgatg agcagccgga
    12001 gattgcgttt gccgttctgg atatagcttt ttacctgcat cagggtaaaa cccgtcgagg
    12061 tcgctatctc ctggtatgag cgtttgtcca ggtaaaacaa agttacacag agtttctgct
    12121 cttttaccag ttcattgagg gcttgttcca tccattctaa gagtcgttct ttttccaggt
    12181 gcaggctgtc gccgccttcc tcgtcccagg cggccagcaa ggaatcggaa agccccgtct
    12241 tgtgaccgtg gcggtcacga atcttcataa ggcaatgatt ccgggcgatg gtatagatcc
    12301 aggatttaaa atactccacc ttgtattttg gcagctcggt aatgaccttg atgaagattt
    12361 gctggacaca gtcgcgggct tcttcctcct ctttaaggta tttcatgcat accccgaaca
    12421 ggagcagggt ataacgttga agaagggccc ccagccattc attgttgtgt tcctcataat
    12481 agcgcgacaa caattcctgg tcggtcatat ggtgcaaagg gtctttcatg cagagagaca
    12541 aatataagat gcttaacggg cgaaattcca cattctaaag tttttgatta attttccgca
    12601 tgatcagagc aatttgttgc gtccccgtca gccctctccg ggcggagccc tcacaccgca
    12661 cagaaatggt cagccagctg atcttcggcg agtgttgcga agtgctggag cagaccaaag
    12721 atcaatggag ccgtgttcgg gtcagatatg acggatatga aggctggtgt acttcccccc
    12781 atttgacgga gatagacgaa aaggagtttg cggcggagcg gacgcccctt acaccggaat
    12841 gggtcgcttc attggaatat aacgggcatc cgatgatggt tcccatgggc agtcatctca
    12901 cggccatgaa gaacgggcgg gcgcaatggc gaaagaaccg ggtccattat aagggaaaga
    12961 cctgggagcc gggagagcgc gccggcgatg ccaaatcgat ccggcagctg acgctgaaat
    13021 tcctgaatgt tccttatttg tggggcggta aatccatctt tggcatcgac tgtagcggtt
    13081 atgcgcagac ggtatataaa tttttgggtg tgccgatctt gcgcgacgcg catcagcagg
    13141 ctggtcaggg cgatccggtc ggttttctgg aagagacgaa gactggtgat ctggcttttt
    13201 tcgacaatga agaaggaagg atcacgcatg tggggatcct gctcaacagt aatgagatca
    13261 tacatgcctc gggaaaggtg aggatcgaca agatcgataa tatggggatc gtgcaatcgg
    13321 acaccgggct gcggacgcat catctgcggc tgatcaaacg tatgctgccg gaagattgag
    13381 gggtggggtg ccgcttccgg gcgggcgatc ggggcgcggt gcgatgctgc cgaatgccgg
    13441 acgatcgggg cggcgcgctt tacttttttc tgaacgtttc gtcatagagc ctgaccgcgt
    13501 cttcgatgac cttcagggcg gtggtcttgt ccccgaattc ctccactacg accgtcttgt
    13561 tctccagttt tttgtattcc tcgaagaaat ggcgcagctc ttcgaagaag tgccggggca
    13621 gctcttccag gttgttgtag tgattgacgc tggggtcgtt ggcagctact gcgatgatct
    13681 tgtcgtcggc atcgccgctg tccaccattt gcatgacgcc catgaccttc gcctgcacca
    13741 ggcagagcgc ctgtacgggc agggaggtga tgaccaggat gtccagcggg tccttgtcgt
    13801 cgccgtaggt ttggggaata aagccataat tgacggggta gtagaaggac gagtagatga
    13861 cacggtccag tttgagcaga ccgctgtcct tatcgatctc gtatttggcc cgggaacctt
    13921 gcgggatctc gatgatcgca tttacgacac gcggagagcc gtcgccgacc ggtactccat
    13981 gccaggggtg tgaaactgtt atcatgagtc aaacctactg catcccttgg gacgaaaaaa
    14041 atttgctttt ggaaagga
//
LOCUS       DASYSV010000381         8732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1356996, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000381 DASYSV010000000
VERSION     DASYSV010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356996"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VGQ51_11685"
     CDS             <1..679
                     /locus_tag="VGQ51_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEV9037280.1"
                     /translation="RIVYLLFNEGYYSRSRDEILRKDLCVEALRLGLLLTEYEMTRLP
                     KTYALIALMCFHSSRFDARVRSSDGEALILYDQQDESLWDQALIAQGMHFLGLSASGG
                     EMSSYHLEARIASWHCLKEDSPEKWEDILELYDRLLKVNYSPGVALNRTYALYKAKGK
                     KEALAAAEQLKGEHNHFYFVLLGELYRGIDDTKARWNFQQAISLARTQTEKRIIEEKI
                     GTLGKNN"
     gene            701..1165
                     /locus_tag="VGQ51_11690"
     CDS             701..1165
                     /locus_tag="VGQ51_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV9037281.1"
                     /translation="MEQRINLYDKGQKAMQALLAFGKRVNSGSIDEHLLNLVALRVSQ
                     INGCAFCLDMHSKDLRAKGDTEQRLYGLNAWREAPYYSDRERAALAWAEAVTTLEGHV
                     PDEVYDTVREQFSETELIDLTMGVIGTNLWNRINVAFRTPAGSYQPGQFATA"
     gene            1192..2043
                     /locus_tag="VGQ51_11695"
     CDS             1192..2043
                     /locus_tag="VGQ51_11695"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV9037282.1"
                     /translation="MKAFLGMGLLGANFVQAMLAKGDQVRVWSRTASKAAQLEKFGAK
                     AFQNITEAVAGADIVHLTLVDDATVDEVLEAARPGLKPGAIIVDHTTTSVIGAVDRTK
                     IWRQLGFPYQHAPVFMGPSNALESTGTMLVSGDQSLIESLGPQLSPMTGKLLNFGAEP
                     GRAAGMKLLGNLFLVSFTAGIADTLGLAKALHIPVSDISALFDTWNPGAMLPGRLKRV
                     ATGDFTQPSWMLSMARKDTRLMLEAAERGGVDLSVIPAIADQMDHWIGEGHGNDDWTV
                     IAKDLRP"
     gene            complement(2059..2469)
                     /locus_tag="VGQ51_11700"
     CDS             complement(2059..2469)
                     /locus_tag="VGQ51_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV9037283.1"
                     /translation="MQTTTITNTSTATSSRARLWTGRILTYLLLLFLLFDAIMKVIKE
                     RHTIETSAKLGWQPDAIQTLGFVLLVSTILYAIPRTAALGAILLTAYLGGAVAASIMS
                     HYPFIFPIIFDVIVWLALWLRDEKLSSHLPVRKN"
     gene            2681..3151
                     /locus_tag="VGQ51_11705"
     CDS             2681..3151
                     /locus_tag="VGQ51_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV9037284.1"
                     /translation="MSNYTPEQNFAALGFSLPPAPVAMGVYKPCMEDGKYLYVSGHGP
                     VCDDNTLIIGRVGQELDAEQGKTAARQVGLTILATVRGHLGSLDRVRRVIKTLGMVNC
                     TSEFERHPYVINGCSELFAQVWGSDNGVGVRSAVGMGSLPNNIPVEIEVLFEIQ"
     gene            3153..4271
                     /locus_tag="VGQ51_11710"
     CDS             3153..4271
                     /locus_tag="VGQ51_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HEV9037285.1"
                     /translation="MNWYTIQNVDEMDTPALVVYPHRVRSNIQKAIAMVGDVARLRPH
                     VKTHKSAEVTQMMLEAGIGRFKCATIAEAEMLAIQGAPDVLLAYQPIGPKAARMVALI
                     NRFPDTRFSCLIDNAGAATAMAAVFAGAGLIVPVWLDINVGMNRTGIAPDEAALQLYQ
                     TATALKGITPVGLHAYDGHIRDTDPAVLEQQCDAAFDRVLALRQQIAALLPGSPLLSV
                     IAGGSPTFPVHARRGSDVQCSPGTFVYWDKGYGDQFPGQPFEPAALVVTRVVSVLGDN
                     RYCLDLGHKSIAPENDLGRRVGFLNGPGLEPTGQSEEHLVVKAKDEGAEIRIGDVFYG
                     VPYHVCPTVALYDRAFTVVDGKVTGEWRNVARDRKLTI"
     gene            4598..5944
                     /locus_tag="VGQ51_11715"
     CDS             4598..5944
                     /locus_tag="VGQ51_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEV9037286.1"
                     /translation="MEVKEPTGRLGVLIPGMGAVATTLIAGVEAVKKGIAQPIGSLTQ
                     MGHLHLNRKNGQLSAADSVQTNYPKIKDFVPLASLQDMVFGGWDIYEENVYEAASRAK
                     VLEPGLLQALRPELEALKPMKGVFDRAYVKNLDGTHVKAAANKYLLAEAVMEDIARFQ
                     DTRGCDRIVMVWCGSTEKYIEVTDVHKSLETFEKGLRENDARIAPSMIYAYAAIKMGI
                     PFLNGAPNLTVDIPALIDLSRRTGTPIGGKDFKTGQTLMKTILAPGLQARALGVKGWF
                     STNILGNRDGYVLDEPDNFRTKEVSKLSVLEDILRPDLSPELYGELYHKIRINYYPPH
                     GDNKESWDNIDIFGWLGYSMQIKINFLCRDSILAAPLVLDLALFGDLAKRAGFSGVQE
                     WLSFYFKSPQTADGSIRPEHDIFKQLTVLQNTLRRLMGEELVNPESLTWAEEEITL"
     gene            5944..6405
                     /locus_tag="VGQ51_11720"
     CDS             5944..6405
                     /locus_tag="VGQ51_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEV9037287.1"
                     /translation="MPLIHRLISTCLGIGYTPKGGGTLAAAAACVVWWFLWRGGNEHF
                     WEEALVTAGLLALGVYSSGKVEPYWGEDNYRVVIDEVAGMFVSLCFLPVKWPCLAAGF
                     VLFRIFDIGKPLYIRRMERLKGGWGVMMDDVLAGLYTNLLLQIVVWFNPSL"
     gene            6402..6797
                     /locus_tag="VGQ51_11725"
     CDS             6402..6797
                     /locus_tag="VGQ51_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV9037288.1"
                     /translation="MISFTKAQIASILATGVDFAVTFLLLRFLGGLVIGGMVAGGVIS
                     GGIGTVCGGVTHFLVSRNWVFQAQEGKWAAQLNRYVLVWTGNLLLNVSGLYLLIHFAG
                     IKAMLAKVIVAVTVAVCYNYILQKRFVFK"
     gene            6805..>8732
                     /locus_tag="VGQ51_11730"
     CDS             6805..>8732
                     /locus_tag="VGQ51_11730"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HEV9037289.1"
                     /translation="MRKTGCGFKGWIIFSLIMAAGWRSHAQEVVLRGLVRDSANGAAL
                     ANASISVKGLRGGARSNGEGRFHIPMTQHAITFTVTMVGFNPGTVHLDSVPDQELVIP
                     LTRQAHVLKTALVKNKKQRYRNKGNPAVELIRQVIDHKNQNRMEAYTYATYQKYEKLV
                     VSVDKVNSKITSNKLLQPYHFLVENSDTTKLEGRRLSPVYLEETYSQNYYRKSPEKTK
                     SVITGKRRVDYGEFIDMQGVSMYLNRLYQDFNIYDNNISVFTNLFLSPIADMAPTFYM
                     YFIEDTVMVDTQKLVRLSFRPRNPNDLLFRGTMWITLDGNYSIQQLRLLVSKSINFNF
                     VREMKIDQDFTRQPDGRYLLTKSDVLGDFGITKNGVGLFGERTVSFLNFKTNTPLPDS
                     LFRGAPTVETDSAGSRNDSFWLAYRADTLSAFENKVYANADSLQHMKSFRRLMDWGTL
                     LLAGYKQAGPFEIGPASTFYSFNPVEGFRLRFGGRTTTRLSTRYYSEAYAAYGFGDQK
                     WKYFLSGTYSLNNKSIYGFPQHYIRASYQKETQIPGQALQFVQEDNFLLSFKRGNNDK
                     WLYNNIARLDYVHELRNHLEYNFGFKWWEQMPAGSISYITVDNSGVDTIKKITTTELS
                     AGIRWAPHEQFYIGKLYRV"
BASE COUNT         2026 a         2122 c         2597 g         1987 t
ORIGIN      
        1 tcgaatagtt tatctgctgt tcaatgaggg atattactcc cgtagcaggg acgagatcct
       61 gaggaaggat ctgtgcgtgg aagcgttgcg gttgggtttg ttgctgaccg aatacgagat
      121 gacccgtctc ccgaagacct atgcgctgat cgcgttgatg tgttttcatt cttcgcggtt
      181 cgatgcgcgt gtaagatcct ccgatggcga ggcgctgatc ttgtatgacc aacaggatga
      241 atcgctgtgg gaccaggcgc tgatcgctca ggggatgcat tttctcggtc tttcggccag
      301 cggcggcgag atgagctcct atcacctcga ggcgcggatc gccagctggc attgtctcaa
      361 ggaggacagc cccgaaaaat gggaggacat ccttgagttg tacgacaggt tgctgaaggt
      421 gaattattct cccggcgtcg cgcttaacag gacctacgcc ctttataaag cgaaaggcaa
      481 aaaagaggcg ctcgctgccg cggaacaatt gaaaggggag cacaaccatt tttattttgt
      541 tttgttagga gaattgtatc gggggatcga tgatacaaaa gcccgatgga attttcagca
      601 ggctatctcg ctggccagga cccaaaccga aaagcggatc atcgaggaga aaattggcac
      661 tttaggaaaa aacaattaat cactggttaa attttctttt atggaacaaa ggatcaacct
      721 ctatgacaag gggcaaaaag cgatgcaggc tctgcttgca ttcgggaagc gtgtgaattc
      781 cggatcgatc gacgagcatc tgctaaatct cgtcgcatta cgcgtctcgc agatcaacgg
      841 gtgcgcgttc tgtctcgaca tgcattccaa ggaccttcgg gcaaagggcg acaccgagca
      901 gcggctgtat gggttgaatg catggaggga agcgccttac tattccgatc gcgagcgggc
      961 cgcgctggcc tgggcggagg ccgtaactac cctggagggg catgtgcccg atgaggtata
     1021 tgacaccgta cgggagcaat tttccgagac ggagctgatc gatctgacga tgggagtcat
     1081 cgggaccaat ttgtggaaca ggatcaatgt ggcgttccgc acaccggcgg gcagttatca
     1141 gccggggcag tttgcgacgg cgtgaattgt cttaccttta acaaaaaggt aatgaaagca
     1201 tttttaggca tgggcctgtt gggcgcgaat ttcgttcagg ctatgttggc caaaggagac
     1261 caggtccgtg tttggagccg gaccgcatcg aaggcggccc aactggaaaa attcggagct
     1321 aaagcatttc aaaatatcac cgaagccgtc gcgggcgcgg acatcgtaca cctgaccctg
     1381 gtggatgacg ccactgtcga tgaggtgctg gaagccgccc ggccgggttt gaagccggga
     1441 gcgatcatcg tcgatcacac gactacatcc gtcattggtg cggtggatcg gaccaaaata
     1501 tggaggcagc ttggttttcc ctaccagcat gcaccggtat tcatggggcc gtccaatgcg
     1561 ctggagagta cgggtaccat gttggtatcg ggtgatcagt ctttgatcga aagtctcggt
     1621 ccgcagcttt ccccgatgac cggcaaactg ctcaacttcg gtgctgaacc cggaagggcc
     1681 gcggggatga aattactggg gaatctgttc cttgtctctt tcacggcggg catcgccgat
     1741 acgctcggac tggcaaaggc cttacacatt cccgttagtg acatctccgc ccttttcgac
     1801 acctggaatc ccggggcgat gctgccgggc cgcttaaaaa gggtggctac gggtgacttc
     1861 acacagcctt cctggatgct ctcgatggcc agaaaggata cgaggctgat gctcgaggcc
     1921 gcggagcggg gaggggtaga tctgtcggtg atccccgcga tcgcagacca aatggatcac
     1981 tggatcgggg aaggtcatgg gaatgatgat tggacggtga ttgcaaagga tctgcggccg
     2041 tgatgccagc ggcggcggtc agtttttgcg aacgggcaga tgtgagctga gcttttcatc
     2101 gcgcagccac agcgcgagcc agactattac atcgaagatg atggggaaga tgaaggggta
     2161 atgagacatg atgcttgcgg cgactgcccc acccagatag gcggtcagaa ggatggcccc
     2221 gagcgctgcc gtgcggggga tggcatacag gatcgtggac accagtaaga cgaatccgag
     2281 tgtttgtatc gcatcgggtt gccatccgag tttcgcgctt gtctcgatgg tgtggcgttc
     2341 tttgatgacc ttcatgatgg cgtcgaacaa tagaaaaagc aggaggagat aagtgagaat
     2401 gcggccggtc caaagtcttg ccctggatga ggtggctgtc gaagtgttgg tgatggttgt
     2461 cgtttgcatg attgtcggtt gagttgttgt tgaatctttt tgacaactca aaattaccga
     2521 cgggatagat acgcggtgat gtgcgaatac gacaattagc aggggcggat gcgtctattt
     2581 accggggctg gtgaaaatat agcgaaataa aaatattcat tgaatcgccc gcgcgcagta
     2641 cattcgctaa cgttgttttc aatccaacat tctttcaatc atgagcaatt acacacccga
     2701 acaaaatttc gctgcgctgg ggttcagtct tccgcctgcg ccggtggcga tgggcgttta
     2761 taagccctgt atggaggacg gtaaatacct gtatgtgtcg gggcatggtc ccgtatgcga
     2821 cgacaacacg ctgatcattg gtcgggtggg gcaggaactg gatgccgagc aggggaagac
     2881 ggcggcgcgg caggtagggc tcacaattct ggcaacggtg cggggacatc tgggaagtct
     2941 cgacagggtc agacgcgtga taaagacgct gggtatggtg aattgcacgt ccgaattcga
     3001 gcgtcatccc tatgtgatca atggctgcag cgagctgttc gcgcaggtgt gggggagcga
     3061 caatggagtg ggggttcgta gtgccgtcgg gatgggatcg ctgccgaaca atatcccggt
     3121 cgagatagaa gtgttgttcg aaatccaata atatgaactg gtacacgatc caaaatgtag
     3181 atgagatgga cacgcctgcg ctggtggttt atccccaccg ggtgcggtcg aacattcaaa
     3241 aagccattgc gatggtcggc gatgtggccc gtctgaggcc gcacgtcaag acgcataaat
     3301 cggcggaggt gacacagatg atgctggagg cgggtatcgg ccggtttaaa tgcgcgacca
     3361 ttgccgaagc ggagatgctg gccatacaag gcgcgcccga tgtgctgctg gcctaccagc
     3421 ctattggtcc caaggcagcg aggatggtgg cgctgatcaa ccggtttccc gacacgcgtt
     3481 tttcctgtct gatcgataat gccggagcgg cgactgccat ggcggccgtt ttcgcaggtg
     3541 cgggactcat cgttccggtg tggctggaca tcaatgtcgg gatgaaccgc acgggcatcg
     3601 ctcccgacga agcggcgttg cagctgtatc agaccgcgac cgcgctgaaa ggcatcacgc
     3661 cggtggggct tcatgcttat gacggacata tccgggatac ggatccggcg gtgctggaac
     3721 aacagtgcga tgcggcgttc gatcgtgtgc tggcgctccg gcagcaaatc gcggccttgt
     3781 tgccgggtag cccgttgtta tccgtcattg ccggcgggtc gccgactttc ccggtccatg
     3841 cccggcgcgg gagcgatgtc cagtgtagtc ccgggacctt cgtctactgg gacaaaggct
     3901 atggcgacca atttccgggg cagcctttcg aacctgccgc gctagtggtg acgcgggttg
     3961 tctcagtatt gggggataac cggtattgtc tcgatctcgg tcacaaatcc atagccccgg
     4021 agaacgatct ggggcggagg gtcggctttc taaatggtcc gggactggaa ccgacgggac
     4081 agagcgagga gcacctggtc gtgaaggcaa aggatgaagg cgcagaaatc cggatcgggg
     4141 atgtgttcta cggtgttccc taccatgtct gtccgaccgt cgcgttatat gacagggcct
     4201 ttacggtggt ggacgggaag gtcaccggag aatggcgcaa tgtggcacgc gaccggaaac
     4261 tgacgatttg agcgacggcc gtccgtagca cgatgaacgc ttggctccgg ccagactgtc
     4321 cgagcaggat ggccccggcc cgatagtcga tggccccggc ccgaccgtct tatttgctat
     4381 ctcatcctct aaaaactgca attcgccgac tttaactatt tccgctgcat aggcggagtt
     4441 aaattgcaat taattcagtg tcacatgtat gtaaatattt ttcgaccaaa aaactatttt
     4501 gttcgaaggt tgtttatttc gataattttg ccgttttatt ctgcaaaagc ggacgctggt
     4561 ccgtataaat attaaccatc atttaaactg tatgaacatg gaagttaaag aaccgacagg
     4621 caggctgggt gtgctgatcc ccggaatggg cgcggtagct acaaccctga tcgcgggcgt
     4681 ggaagctgta aagaaaggta tcgctcaacc gatcgggtct ctcacccaga tgggccatct
     4741 ccatctgaac cggaagaatg ggcagctatc ggcagcagat tccgttcaaa ccaattatcc
     4801 gaagatcaag gatttcgtac cgctggcctc gctgcaggac atggtcttcg gcggttggga
     4861 catctatgaa gaaaacgtct atgaagctgc cagtcgtgca aaggtcttag agcccggttt
     4921 actgcaggct ctgcgtcccg agctcgaggc gttgaagccg atgaaaggag tattcgatcg
     4981 cgcctatgtc aagaaccttg acggaacgca tgtcaaagcg gcggccaaca agtatcttct
     5041 ggccgaagcc gtcatggaag atatcgcccg gttccaggac acccgcggct gtgaccggat
     5101 cgtaatggta tggtgcggca gcacggaaaa gtatatcgaa gtgacggatg tgcataagag
     5161 tcttgagacg tttgaaaaag ggcttcgtga aaatgatgcg cggatcgcgc cctccatgat
     5221 ctatgcctat gccgcgataa agatgggtat ccccttcctg aatggcgcac ccaatctgac
     5281 ggtagacata cccgcgctga tcgatctgtc gcgacgcacc gggactccca tcggaggaaa
     5341 ggactttaag accggtcaga cactgatgaa gaccatcctg gcccccggct tacaggcccg
     5401 cgcgctggga gtgaaaggct ggttctccac gaatatcctg ggtaacaggg acggttacgt
     5461 gctggatgag cccgataatt tccgtaccaa ggaagtttcg aagctaagcg tcctcgagga
     5521 tatccttcgg ccggatctca gtcccgaatt gtacggcgaa ctctatcata agatccgcat
     5581 taactattat ccccctcatg gagacaataa ggaaagctgg gacaatatcg acatcttcgg
     5641 atggctgggt tattcgatgc aaataaagat caacttcctt tgcagggaca gcatactcgc
     5701 ggcgccgctg gtattggatc tggccttgtt cggcgatctg gcgaagaggg cgggtttcag
     5761 tggagtacag gaatggttgt cattctattt caaatcgccg cagacggccg atggatccat
     5821 acgtccggaa catgacatct tcaaacaatt gacggtgctt cagaacacac ttcgccgatt
     5881 gatgggtgag gaactggtca acccggaaag cctcacctgg gcggaggaag aaataacgct
     5941 ctgatgcccc tgatccatcg actcatttcc acctgtctgg ggatcggcta cacgcctaaa
     6001 ggagggggta cccttgcagc ggctgctgcc tgtgtcgtgt ggtggttctt atggaggggt
     6061 ggaaatgagc atttttggga ggaagcgctt gtcacggcag ggctgctggc cctcggggta
     6121 tacagctccg ggaaagtaga gccttattgg ggcgaggaca attatcgtgt cgtcatcgat
     6181 gaggtggcgg gaatgttcgt aagtctttgt tttctaccgg tgaaatggcc gtgcctggcg
     6241 gccgggttcg tgttgttcag gatctttgat attggcaagc ctctgtatat ccgcaggatg
     6301 gagcgactga agggtgggtg gggcgtaatg atggacgatg tgctggctgg cttatacact
     6361 aaccttttgc tgcagatcgt tgtatggttc aacccttcat tatgatcagc tttaccaagg
     6421 ctcaaatagc atccatactg gcaaccgggg tcgatttcgc ggtcaccttc ctgttgctgc
     6481 gttttttggg cgggctggtc atcgggggca tggtggcagg cggagtgatc agcggcggca
     6541 tcggcaccgt ttgcgggggt gtaactcatt tcctggttag ccggaactgg gtatttcagg
     6601 cacaggaggg aaaatgggcc gcacagctca accgctatgt gctggtctgg acgggcaatc
     6661 tgctgctcaa cgtttccgga ttatatttgt tgatccattt cgcagggatc aaggcaatgc
     6721 tggctaaggt aatcgtagcc gtaacggttg ccgtatgtta taactatata ctacagaaga
     6781 gatttgtatt taaatagcaa tcggatgaga aaaactggat gtgggtttaa aggctggata
     6841 atcttctcgt tgataatggc cgccggttgg cgtagccatg cgcaggaggt cgtactgcgc
     6901 gggctggtca gggattccgc caacggagcc gcactcgcca atgcgagcat ttcggtgaaa
     6961 gggttacgcg gaggcgcaag gagcaacggc gaaggccgtt ttcacattcc aatgacgcaa
     7021 catgccataa ccttcaccgt cacaatggtc ggtttcaacc ccgggacggt gcatttggat
     7081 tcagtgccag accaggaact ggtcatcccg ctgacaagac aggcgcacgt attgaaaacc
     7141 gccctggtga agaataaaaa gcagcggtat cgcaacaagg ggaatccggc ggtggagctg
     7201 atcagacagg tcatcgatca taagaaccaa aaccggatgg aggcttatac ctacgccacc
     7261 tatcagaaat acgaaaagct ggtcgtctcg gtcgacaagg tgaattcgaa gatcaccagc
     7321 aacaagctgc tccagcctta tcatttcctc gtagagaaca gcgataccac taagctggaa
     7381 ggccggcggc tttctcctgt ctatctcgaa gagacctatt cgcagaacta ttaccggaag
     7441 agcccggaaa agaccaaatc ggtgatcacc ggcaagcgga gggtagacta tggcgaattc
     7501 atcgatatgc agggcgtgag catgtatctc aatcgtcttt accaggactt caatatctat
     7561 gacaacaata tctccgtttt taccaatctt ttcctgagcc cgatagccga tatggcgccg
     7621 acattctata tgtattttat agaggacacg gtcatggtgg acactcagaa gcttgtccgg
     7681 ctgtccttcc gtccccgcaa tcccaatgac ctgcttttcc gcgggacgat gtggatcacg
     7741 ctggacggca attattccat tcagcagctg cgtctgctgg tgagcaagag catcaacttc
     7801 aacttcgtgc gcgagatgaa gatcgaccag gattttaccc gtcagccgga cggccgttat
     7861 ctgctgacga aaagtgacgt gctgggggat ttcggcatta ccaaaaatgg ggttggtctt
     7921 ttcggcgaac gaacagtttc ttttctgaac ttcaagacca acacgccact gcccgacagt
     7981 cttttcaggg gtgcgcctac ggtcgagacg gacagcgccg ggagccggaa tgacagtttt
     8041 tggctggcct accgagccga taccctgtcg gcattcgaga acaaggtcta tgctaacgca
     8101 gacagtctgc aacatatgaa atcgttccgc cggctgatgg actggggtac gctgctgctc
     8161 gccggttata aacaggccgg gccgttcgag atcgggcctg cgagtacttt ttacagtttc
     8221 aatcccgtag aaggattccg tcttcgattc ggcggcagga cgaccacgcg gttgagcacg
     8281 aggtattatt cggaggccta tgccgcttat gggtttggcg atcaaaagtg gaaatacttc
     8341 ctgagcggca cttacagcct taataacaaa tccatctacg gattcccgca gcattatatt
     8401 cgcgcatctt atcagaagga gacccaaata cccggacagg cactgcagtt cgtacaggaa
     8461 gacaacttcc tgctatcctt caagcgcggc aataacgaca aatggcttta caataatatc
     8521 gcgcggctgg actatgtcca cgagctgcgc aaccatcttg aatacaattt cggtttcaag
     8581 tggtgggaac agatgccggc gggttctatc agctatatta cggtggacaa cagcggcgtc
     8641 gatacgataa aaaagatcac cacgaccgaa ctttcggccg ggatacgctg ggcgccgcat
     8701 gaacagttct atatcggcaa gctctaccgc gt
//
LOCUS       DASYSV010000382         9466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1738490, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000382 DASYSV010000000
VERSION     DASYSV010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738490"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGQ51_11735"
     CDS             <1..222
                     /locus_tag="VGQ51_11735"
                     /inference="COORDINATES: protein motif:HMM:NF027575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HEV9037290.1"
                     /translation="YDILKDGRLLFHQNIMPGLSGNKVFRSKEDALVVGKVVYDRMLA
                     GQTPAITEKEMRELHVYIPPDTAAADTSK"
     gene            287..2878
                     /locus_tag="VGQ51_11740"
     CDS             287..2878
                     /locus_tag="VGQ51_11740"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV9037291.1"
                     /translation="MRNSFLAVLFAFCAGSVLAQTPSHFDQHKLWDPQFYPSNGTVYR
                     SAGGAPGAQYWSNRADYTIHTVLDTAQHSLSGDVVVTYTNNSPDELPFLWLQLDQNIY
                     REDSRGEATNPVTGGRWSNRKFTDGDVIRSVTIVRDGKETKAEWLVSDTRMQIKLPEA
                     LRASGGVIKFRIDYSFSIPEYGTDRMGRQLVADGWIYEIAQWYPRMEVYDDVTGWNVM
                     PYLGQGEFYLEYGNIDYTITAPSNLVVVGSGELLNPQEVLTASQQSRLAQARASDKTV
                     VIKSENEVKAGTGHLPKASLTWHFRCNQTRDVAWAASRSFIWDAARINLPGGKKALAQ
                     SVYPSESADPKAWGRSTEFVKGCIELYSQEWFPFTYPVATNVAGLVAGMEYPGIVFCG
                     GDQRGGGLWDVTNHEFGHNWFPMIVGSNERKYAWMDEGFNTFINGVDTRVFNHGEFNH
                     KEDEQRAAMYMFNERSEPLFTIPDVIGAANLGTAAYAKPALALDLLRKYVLGEKRFDY
                     AFRTYIRRWAFKHPTPWDFFRCMENAGGEDLSWFWRGWVLNNWKLDQGVKDVKYVQDD
                     PSKGAIITIENLEEMAMPVVMAIQQESGKTDTITLPVEIWQKGGTKAFAYPSTSKIKS
                     IIIDPGHDFPDINPSNNTWTGQALERPVPPGVSAAEVVNKYLVAVGGKDKLEAVKDLQ
                     VVATATVQGQKVIETLMSVLPNDFSEDIELPDMNMHAVHILVKGDSVTIVQGGKSPTL
                     DDETKRELREEANPFPELNFSKPGYKTDLTSIKNINGKDNYELKVTNPSGKVFSYYYD
                     VNTGLRTRLVKNLRQGQTVTEYGDYRDVSGIKFPWHVDDNQGEVDLDMTVTSIKVNAG
                     LTAADIK"
     gene            complement(2916..4028)
                     /locus_tag="VGQ51_11745"
     CDS             complement(2916..4028)
                     /locus_tag="VGQ51_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEV9037292.1"
                     /translation="MADINYSNFTLGIEEEYMVIDPKTLELKSHEQKIVTEGQKIIRD
                     KVKAEMHQAVVEVGTDICRDIHEARKDVSTLRSTISKIAEDNGFKVGAAGTHPFSHWE
                     SQLITDHARYNEIVNELQEAARSNLIFGLHVHVGMETRQMANHIANSTRYFLPHIYAL
                     STNSPFWEGRRTGYKSFRTKVFDKFPRTGIPDYFESIEAYDNFVKLLVKTNCIDNAKK
                     IWWDLRVHPFFNTVEFRICDVPMTIDETICIAALFQAVCAKIYKLRSRNMNYIQYSRA
                     LINENKWRASRYGIDGHLIDFGKEEEVNTRVLIYELLDFIDDVVDPLDSREATHYAKH
                     MLETGTGADRQLKVFEETGSLVKVMDYIAGQFLAGA"
     gene            complement(4038..5000)
                     /locus_tag="VGQ51_11750"
     CDS             complement(4038..5000)
                     /locus_tag="VGQ51_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037293.1"
                     /translation="MSVSKKIGILYGRERSFPMALIEAINRKNVPGITAEPVRIDKVA
                     QARDSGYAVIIDRISQDVPFFRAFLKNAALCGTAVINNPFWWSADEKFFNNCLAEKIG
                     VPVPKTVILPSRDLPPDTTDQSFSNLAFPLDWESIFRYVGFPAYMKPFAGGGWKNVYK
                     LHDADEFYQRHAETGQLVMLLQEEIIFDEYYRCYCIGGKYVRVMPYEPRNPHHLRYQA
                     AFSPSPERLRQMEEIVLRINAWLGYDFNTVELALRDGIPYAIDFCNPAPDAEKTSVGE
                     DNFDWVVGTSADYAVERALAHRPGIDNLTWGNHLRYSIAQQKID"
     gene            complement(5031..5759)
                     /locus_tag="VGQ51_11755"
     CDS             complement(5031..5759)
                     /locus_tag="VGQ51_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEV9037294.1"
                     /translation="MDLDEEYHRWHSPVIGRDFEMLVFGHSGYPVILFPTSQGRYFQN
                     KDQGLIETARWFLENGKIRIYCPDSLDSASWYNKSVPPAERARQHALYDRLILEEIAS
                     RATRDTGHERIALAGCSFGGYHSANFSFRHPHLAAYCFSMSGVFDIRSFTDGYYDDNI
                     YFNNPVDYIVDASDPALWKMGFVLGTAEHDICRGQNEWMSRLLSAKSIPHWLDIRAGR
                     DHDWQVWKEMFPQYISQLPGASGS"
     gene            5819..6625
                     /locus_tag="VGQ51_11760"
     CDS             5819..6625
                     /locus_tag="VGQ51_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV9037295.1"
                     /translation="MSIEKTTRRVSISIPSLHLDRIVETDFFLPMISEASRDVSLLLI
                     NDGQDLGTVGLEGMLDDLYERELIRPLICVGIHAGADRKMEFGTAGQPDYLGRGVKAE
                     AYTRFVFEELIPMTRRRWGIPVFREKAFAGFSLGALSAMDIVWRHPEEFARVGLFSGS
                     FWWRSRDKGDANFDEARDRIMQREVREGGYYPWLKFFFECGTEDEKEDRNGNGVIDSI
                     DDTKDLIAELVVKGYDPVKDIRYLEIEGGDHSVETWARAMPEFLLWGWGK"
     gene            complement(6682..>9466)
                     /locus_tag="VGQ51_11765"
     CDS             complement(6682..>9466)
                     /locus_tag="VGQ51_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037296.1"
                     /translation="DGRMNNVTVDGANLNNNFGLSTDLLPGSGNPIALDALQEISINL
                     SPFDVKQSGFTGANIAAITKSGTNDFHGSAYWAYRNQKYNGTNVDGFKLPNPAKTSNK
                     IYGATFGGAIIKNKLFFFVSGELEQNSKPGVTYYPAGGSGNGIKSNVSIDSLQKLSSY
                     LQSQYHFNPGAYDNFPTFDGKNHKLLGRIDWNISNVHKLTAKYSDYSSTQYFTISQSG
                     GINGANGGAGVGTVYVPRFGTQAMSFQNVNYNQQDKVKSGSLELNSNWQGRFANQFIA
                     TYTRITTFKGHDGPTFPFVDIMAADKTDYLSFGNEPFNANNNKVINNVLTLTDNFTYF
                     AGKHTLIAGVNYEYQKVGNMFMPGSQGYYIYNSLDDFINNRAPALFSINYSLVPGQDA
                     VYSANLEVGQLGVYIQDDYVVNPRLKITAGLRVDAPIFPQAPLENPAISALTLQDKNG
                     QPAHYNDGQWPKGSLYFAPRVSARWDVHGDKSLIVRGGTGIFTGRVPFVFLTNDPSTS
                     GMYTFGATVKAANANMSNFLFNPDPHAYNPFYNNGLNPAQFPTKAGSVAPGSFAVLNR
                     NFKFPQVWRTDVAVDKAVGNGWTVTAEALFTKNINDPFLRNSAQKAPDATVTVGANNT
                     RGYYSNSAARYVIGPSVTQAIVLENNSQGYSMSLTAQAARSFSHGFYASLAYTYTMAK
                     DVSANPGSQAASAWSANSTSGTLNDAQLSYASFSVPHRIVGTFSYRLEYAKHLASTFS
                     LYYEGAAQGRFTYIYSGDINDDGYSSSDLLYIPKDPTDIHFVDLAASGSTPAFTAQQE
                     SDAFFSFIGQDKYLSKHKGEVAKRNAVVEPWYNRVDFAFAQDLFTNIGKNKSTLQFNV
                     SVINFLNLLSHNWGVKSFYVLNNPLKVNKVVNGVPYFQMAAYNGALLNKTYINDNSTN
                     STWGLQIGLKYMF"
BASE COUNT         2059 a         2182 c         2971 g         2254 t
ORIGIN      
        1 tacgatatcc tcaaggatgg aaggctgttg ttccatcaga atatcatgcc gggtctcagc
       61 ggaaataagg tgttccggag caaagaagat gccctcgtcg taggaaaggt ggtttatgat
      121 cgaatgctgg ccgggcagac gccggcgata acggaaaagg agatgcgtga gttgcatgtg
      181 tacataccgc cggacacggc cgccgcagac acttcgaaat agttattttg atattaatga
      241 gacgagcccc gcgcaaggat tgcgcggggt tctttatctt tagcccatga gaaattcatt
      301 tttagccgtc ctgtttgcgt tttgtgccgg gagtgtcctg gctcagacgc cctcgcattt
      361 cgatcagcac aaattgtggg atccgcaatt ttatccttcg aacggaaccg tttaccggag
      421 tgcgggcggc gctcccgggg cacagtattg gtccaaccgt gccgattata cgatccatac
      481 ggtgctggat acggctcagc attctttgag cggtgacgtc gtggttacct acaccaataa
      541 tagtccggat gagctgccat ttctctggct gcagctggat cagaatattt atcgggagga
      601 ttcgagaggt gaggccacga acccggtaac gggcggaaga tggtcgaaca ggaaattcac
      661 ggatggggat gtaataaggt cagtgacgat tgtgcgtgac ggaaaagaga cgaaggccga
      721 gtggttggtt tcggatacgc ggatgcagat aaagttgccg gaggcgcttc gcgcaagtgg
      781 cggagttatc aaattccgga tcgattattc gttttcaata ccggaatatg ggaccgacag
      841 gatgggacgt cagctggtgg ctgacggatg gatctatgag attgcgcaat ggtatccgcg
      901 gatggaggta tatgacgacg taaccggctg gaatgtgatg ccttatctcg gtcagggcga
      961 gttttacctg gaatatggca atatagatta tacgataact gctccttcga acctcgtagt
     1021 ggtggggtcg ggtgagctgt tgaacccgca ggaggtattg acggcctcgc agcaaagccg
     1081 tttggcgcag gccagggcga gtgacaagac cgtggtcata aaatcggaaa atgaagtgaa
     1141 ggcgggcacg ggtcatttgc cgaaagcgtc gctgacctgg catttccggt gcaatcagac
     1201 gcgggacgtg gcctgggccg catcgcgttc gtttatctgg gatgcggcgc ggatcaatct
     1261 tcccggtggg aagaaggcac tggcgcagtc ggtgtatcct tcggaatccg ccgatccgaa
     1321 ggcctggggg aggagcactg aattcgtgaa gggatgtatc gagctgtatt cccaggaatg
     1381 gtttcccttt acgtatccag tggctacgaa tgtcgcgggt ctcgtcgccg gtatggaata
     1441 tcccggtatc gtcttttgcg gcggtgatca gcggggcgga ggtctttggg acgtgacgaa
     1501 tcatgagttc ggtcacaact ggttcccgat gatcgtcggg tccaatgagc ggaagtatgc
     1561 gtggatggat gaaggattca atactttcat caatggtgtc gacaccaggg tgttcaatca
     1621 tggggagttc aatcataaag aggatgagca gcgcgccgcc atgtacatgt tcaatgaaag
     1681 gagtgagccc ctctttacca tccccgatgt gatcggggcc gctaacctgg gcaccgcggc
     1741 atatgccaag ccggccctgg cgttggatct ccttcggaag tatgtgttgg gggaaaagcg
     1801 gttcgattac gcgttccgca cttatatccg gcggtgggcc ttcaagcatc cgaccccctg
     1861 ggatttcttc cgttgtatgg agaatgcggg cggagaggat ctgagctggt tctggcgggg
     1921 ctgggtcctc aacaactgga aactggatca gggtgtgaag gacgtaaaat atgtgcagga
     1981 tgatccgtcc aaaggggcga tcatcacgat cgagaatctc gaggagatgg cgatgccggt
     2041 ggttatggcc atccagcagg agagtgggaa gaccgacacg attactctgc cggtagagat
     2101 ctggcaaaag ggggggacga aggcgttcgc ttatccttcc acatccaaga tcaaatccat
     2161 catcatcgat cccgggcatg atttcccgga tatcaatcct tcaaacaata cctggacggg
     2221 acaggcattg gagcggccgg tacctccggg cgtgtcggcg gccgaagtcg taaataaata
     2281 cctggtcgct gtcggcggga aggacaagct ggaagccgta aaggatctgc aggtcgtcgc
     2341 aaccgcgacc gtacagggac aaaaggtgat cgagacgctt atgagcgttc tgccgaatga
     2401 tttttcggag gatatagaac ttccggatat gaacatgcat gccgtgcata tcctggtaaa
     2461 gggcgacagt gtaacgatcg tccagggtgg gaagtcgccg acgttggatg atgagacgaa
     2521 aagggaactc agggaggaag cgaatccttt cccggaactg aatttttcga aacccggtta
     2581 taagaccgat ctgaccagca tcaagaatat caatggcaag gacaattatg agctgaaggt
     2641 cacgaatcct tcgggcaagg tgttcagcta ttattacgat gtgaatacgg gcctgaggac
     2701 gcggctggtc aagaatctca gacagggtca gaccgtcacg gaatatggtg actatcgcga
     2761 tgtgagcggg atcaaattcc cgtggcatgt ggatgataac cagggtgagg tcgatctgga
     2821 tatgacggta acgtcgatca aggtcaatgc ggggttgacg gcggccgaca tcaaatagca
     2881 gtctgcgcgg cggcgcgcgg aagcgtgccg ccggatcagg cgcccgccag gaactggccg
     2941 gcgatatagt ccattacctt cacgaggctg ccggtctctt cgaagacctt cagctggcgg
     3001 tcggcgccgg tgccggtttc cagcatatgt tttgcgtaat gggtcgcctc gcggctgtcc
     3061 agcgggtcta ctacatcgtc gatgaaatcc agcagctcgt agatgagaac gcgggtattg
     3121 acttcttctt ctttcccgaa atcgatgagg tgtccgtcga tgccatagcg gctggcgcgc
     3181 catttgttct cattgatgag cgcccgggaa tactggatat aattcatgtt ccgcgagcgc
     3241 agcttataga tctttgcgca tacggcctgg aagagcgcgg cgatgcagat ggtttcgtcg
     3301 atggtcatcg gcacatcgca gatcctgaat tcgacggtat tgaagaaggg atgtacgcgg
     3361 aggtcccacc agatcttttt ggcattgtcg atacaatttg tctttaccag cagcttcacg
     3421 aaattgtcat aggcttcgat gctttcgaaa tagtctggga tgcccgtgcg cgggaatttg
     3481 tcgaatacct ttgtccggaa ggacttatag ccggtgcgtc ttccttccca gaacggggaa
     3541 ttggtgctca gcgcatagat atgggggagg aaatagcggg tggaatttgc gatatggttg
     3601 gccatctgcc gggtttccat tcccacgtgg acgtggagac cgaagatgag gttgctgcgc
     3661 gccgcctcct gcaattcatt gacgatctcg ttatatcgtg cgtgatcggt gatcagctgg
     3721 ctttcccaat ggctgaaggg gtgggtacct gccgcgccga ctttgaaacc gttgtcttca
     3781 gcgatcttcg agatcgtgga gcgtagggta gacacatcct tgcgcgcctc gtgtatatcc
     3841 cggcagatgt cggtgcctac ttccacaacg gcctggtgca tctctgcctt gaccttgtcg
     3901 cggatgatct tttgtccttc ggtcacgatc ttttgttcat ggcttttcaa ttccagtgtc
     3961 ttcgggtcga tgaccatata ttcctcttcg atgcccaggg taaagttgct atagttgatg
     4021 tctgccattt ggtgaaatta gtcgattttt tgttgtgcga tgctataacg gagatggtta
     4081 ccccaggtaa ggttgtcgat gccggggcgg tgggccaggg cccgttcgac ggcgtagtcc
     4141 gcggaagtcc cgacgaccca gtcgaaatta tcctctccca cgctggtctt ttccgcatcc
     4201 ggcgcggggt tgcagaaatc gatggcataa gggatgccgt cgcgtagggc cagttcgacg
     4261 gtattgaagt catagccgag ccaggcattg atccgaagaa cgatctcctc catttgacgg
     4321 aggcgttcgg gggaaggaga gaatgcggcc tgataacgaa ggtggtgcgg gttgcgcggc
     4381 tcatagggca tcacgcggac atattttcct ccgatacaat aacaacggta atattcatcg
     4441 aaaatgatct cttcctgcag gagcatcaca agctgtcccg tctcggcgtg acgttgatag
     4501 aattcgtccg cgtcgtgcag cttgtataca tttttccagc cgcctccggc gaagggtttc
     4561 atataggcgg ggaagccgac gtaacggaat atggattccc agtcgagcgg gaaggctaag
     4621 ttggaaaagg attgatcggt agtgtcgggt ggcaggtcgc gggaaggcag gatgacggtc
     4681 ttggggacgg ggacgccgat cttttcggcg agacaattgt tgaagaattt ttcatcggcg
     4741 ctccaccaaa acgggttatt gatcacggcc gttccgcaga gggcggcatt tttcagaaag
     4801 gcccggaaga agggtacgtc ctgcgagatc cggtcgatga taaccgcgta gccggagtcg
     4861 cgtgcctgtg ctaccttgtc gatgcggacg ggttccgccg taatgccggg aacattctta
     4921 cggttgatgg cctcgatgag ggccatgggg aagctgcgtt cgcggccgta gaggattccg
     4981 atttttttgc tgacagacat gttgtgcgat ttttcgggtg tgtgatgaag ttaggagccg
     5041 ctggcgcccg ggagttgaga aatgtactgg gggaacattt ctttccatac ctgccagtca
     5101 tgatcgcgac ccgcgcggat gtccagccaa tgggggatgg atttggccga aaggaggcgg
     5161 ctcatccatt cgttttggcc ccggcagatg tcgtgttcgg cggtacccag tacgaagccc
     5221 attttccaga gggcggggtc ggaggcgtcg acgatatagt cgacgggatt attgaaatag
     5281 atgttgtcgt cataatagcc gtcggtgaat gaccggatgt cgaaaacgcc gctcatggag
     5341 aagcagtagg cggcgagatg ggggtgccgg aaggagaaat tggccgaatg ataaccgccg
     5401 aaactgcagc cggcgagggc tattcgttcg tggcccgtat cccgggtggc gcgggaagcg
     5461 atctcttcga gtatcaggcg gtcgtagagg gcatgttgcc gggcgcgttc ggccgggggc
     5521 acggatttgt tgtaccagga tgcagaatcg aggctgtcgg ggcaataaat cctgatttta
     5581 ccgttttcaa ggaaccagcg ggccgtttcg atgagtccct ggtctttgtt ttggaagtat
     5641 ctgccctggg aggtggggaa gagtatgaca ggatagccgg aatgaccgaa gacgagcatt
     5701 tcgaagtcgc ggccaataac gggtgaatgc cagcggtgat attcttcgtc caggtccata
     5761 ataatgatgt ggggaacggg ctccgcccgt tctaggtaaa tatactattt ttgacgctat
     5821 gtcgattgaa aagactacaa gacgggtaag catatccata ccatcgttgc atctcgaccg
     5881 tatcgtcgag acggattttt tcctgcctat gatatcggag gccagccggg atgtaagcct
     5941 gttattgatc aatgacgggc aggatctggg tacggtgggg ctcgagggca tgctggacga
     6001 cctgtatgag cgggaactga tccgtccgtt gatctgcgtc ggtattcatg ccggcgccga
     6061 caggaagatg gaattcggga ctgcggggca gccggattac ctgggaaggg gagtgaaggc
     6121 ggaagcgtat acgcgtttcg ttttcgaaga gctgatcccc atgacccgcc ggcgttgggg
     6181 cataccggta ttcagggaga aagcgtttgc cggctttagc cttggggcgt tgagcgccat
     6241 ggatatcgtg tggcgtcatc cggaggaatt tgcgcgggtg ggtcttttct cgggcagctt
     6301 ttggtggagg agcagggata agggagatgc gaattttgat gaggcgaggg atcggatcat
     6361 gcagcgggaa gtgcgtgagg gcggatatta cccctggttg aaattctttt tcgaatgcgg
     6421 tacggaggac gagaaggagg atcggaatgg gaatggggtt atcgactcga tcgacgatac
     6481 gaaggacctg atcgccgaat tggtggtgaa gggctatgac ccggtgaagg atatccgcta
     6541 tctcgagatc gagggcgggg accattctgt agagacctgg gcgagggcta tgccggaatt
     6601 tttgttgtgg ggatggggaa aataagatcg ttataaaaaa acccggctcg aggccgggtt
     6661 tttgtttatg tgcacaggac tttagaacat atacttcaaa ccgatctgca gcccccaggt
     6721 gctgttcgtc gagttatcgt tgatataggt cttgttcagc agagcgccgt tatacgcggc
     6781 catctggaaa tacggaacgc cgttgacgac cttattcacc ttcagcgggt tgttcaggac
     6841 atagaaggat ttgacccccc agttatgact gagcaggttc aggaagttga tcaccgaaac
     6901 attgaactgg agcgtgctct tgttttttcc gatgttggtg aacaggtcct gtgcgaacgc
     6961 gaagtcgacc ctgttgtacc atggttcaac gacggcgttg cgtttggcga cttcgccttt
     7021 gtgtttgctc aggtatttat cctgcccgat aaaggagaag aaagcgtccg actcctgctg
     7081 ggccgtgaac gcaggtgtac ttccggatgc ggccaggtcg acaaaatgga tatcggtcgg
     7141 gtccttcgga atatagagca ggtccgaact cgaatagccg tcatcattga tatcaccgct
     7201 atatatatag gtaaagcgcc cctgggccgc tccctcataa taaaggctga aggtcgaagc
     7261 cagatgtttg gcatactcca ggcgatagga gaacgtgccg acgatccggt gagggacgga
     7321 aaaggaagca tacgacaatt gcgcgtcgtt gagcgtgccg gaagtcgagt tggcactcca
     7381 ggccgatgca gcctgtgaac cggggttggc cgatacgtcc ttcgccatgg tataggtgta
     7441 agccagcgag gcatagaagc catgcgaaaa gctcctggct gcctgggccg tcagggacat
     7501 agaatagccc tggctgttat tctcaagaac gatcgcctgc gtcacgctgg gtcctatcac
     7561 ataacgtgct gccgagttgg agtagtagcc gcgggtgtta ttggctccga cggtaaccgt
     7621 cgcatcgggc gccttctgag ccgaattacg caggaaggga tcgttgatat tcttggtgaa
     7681 aagggcttct gcggtcaccg tccagccgtt gccgacggcc ttgtctacag cgacatcggt
     7741 ccgccatacc tgcgggaatt tgaaattacg gttcagcacc gcgaagctgc ccggcgcgac
     7801 gctgcctgcc ttggtgggga actgtgccgg attgaggccg ttgttataaa agggattgta
     7861 cgcatgcgga tccgggttga agaggaaatt gctcatgttg gcattggctg ccttcacggt
     7921 ggcgccgaac gtgtacatgc cgctggtgct cggatcgttg gtcaggaata cgaaggggac
     7981 gcgacctgta aagatgccgg tcccaccgcg aacgatcagg ctcttgtcgc catggacgtc
     8041 ccagcgtgca gaaacgcggg gtgcgaaata cagtgaacct ttcggccatt gtccatcgtt
     8101 gtaatgcgcc ggctgaccat tcttgtcctg aagggtgagc gcggagattg ccgggttttc
     8161 cagcggcgcc tgcgggaaga tgggggcgtc gacgcgtaga ccggcagtta ttttcagccg
     8221 gggattgacg acatagtcgt cctgaatata tacacccagt tgtccaactt ccaggttcgc
     8281 ggagtaaacg gcgtcctgac cgggaacgag cgaatagttg atggagaaaa gtgcgggagc
     8341 gcggttattg ataaagtcgt cgagcgaatt gtagatatag tatccctgcg aacccggcat
     8401 gaacatattg cccacctttt gatattcata gttgacgccg gcgatcaatg tgtgcttccc
     8461 ggcaaaatag gtgaagttgt ccgtcagggt cagcacattg ttgatcactt tgttattgtt
     8521 ggcgttgaac ggttcgttgc cgaagctgag ataatcggtc ttgtcggccg ccatgatatc
     8581 gacgaagggg aaggtcgggc cgtcatgccc tttaaacgtc gtgatccggg tatatgtagc
     8641 gatgaactgg ttggcgaatc tgccctgcca gttgctgttc agttcgagag accctgattt
     8701 caccttatcc tgctggttgt agttgacgtt ctggaacgac atggcctggg ttccgaaacg
     8761 cggaacatat accgtgccga cgcctgctcc gccattggcg ccgttgatgc cgccgctctg
     8821 gctgatcgtg aaatattgcg tactagagta atcgctgtac ttggcggtca gcttatggac
     8881 gttgctgata ttccaatcga tcctgcccag caatttatgg ttctttccat cgaaggtcgg
     8941 gaagttgtcg taagcgccgg gattgaagtg atactggctt tgcagatagc tggacagctt
     9001 ttgaagcgaa tcaatggaaa cattcgattt gataccgttg ccggagccgc ctgccgggta
     9061 gtaggtgaca cccggtttgc tgttctgctc gagctcgccg ctgacaaaaa agaagagctt
     9121 gttcttgatg atcgctccgc cgaatgtagc cccatagatc ttgttgctcg tcttggcggg
     9181 gttaggcagc ttgaaaccgt cgacattcgt accgttatat ttttggttgc gatacgccca
     9241 gtatgcgctg ccatggaaat cgttggtacc gctcttggtg atagccgcga tgttggcgcc
     9301 tgtgaaaccg gattgcttta cgtcgaaggg ggagaggttg atggatatct cctgtaaggc
     9361 gtcgagcgcg atcgggttgc ctgagccggg cagcaggtcg gtgctgagac cgaaattgtt
     9421 attgaggttc gcgccgtcta cggtgacgtt gttcatacgg ccgtcg
//
LOCUS       DASYSV010000383         6622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2121071, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000383 DASYSV010000000
VERSION     DASYSV010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121071"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VGQ51_11770"
     CDS             <1..824
                     /locus_tag="VGQ51_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9037297.1"
                     /translation="RILSDRQRLRQILINLVGNAVKFTEKGEIRVRVFLPPTSSMPDI
                     PAGHIQIAFEIQDTGIGILPGQLNRLFQSFSQVDSSVTRKYGGIGLGLSISNKLVHLM
                     NGQITAESAAGVGSVFMFTITTRPAPLRSSNPAAVAIPDLAAKYPLRILLAEDNPINQ
                     QLAMITLNKMGYSPAVAANGREAVERLKQESFDLIFMDVQMPEMDGLEATRHIRQKGD
                     RQTVIIAMTANATIQDRDDCLAEGMNDYLCKPVDLGELIFTLEKWGHQINKKAAS"
     gene            1037..1450
                     /locus_tag="VGQ51_11775"
     CDS             1037..1450
                     /locus_tag="VGQ51_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037298.1"
                     /translation="MKAVARIIAVALSTIVILVSNPLASQANGGRTDSKKALTEEKVS
                     IKYVGSENGQVVFRVEFENPTSEKFSLIIRNDNGDVVYHQQFNDTHFSKNVFIANVDS
                     DIQPTFVIRSADNSEIVRQFRVSKTVTENTTVTRL"
     gene            complement(1497..1868)
                     /locus_tag="VGQ51_11780"
     CDS             complement(1497..1868)
                     /locus_tag="VGQ51_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037299.1"
                     /translation="MRKTLAILLFLSLGGFIQSRAQSLKNSEWKFYVQGLNDTLTYHF
                     DADTSWAASTTGDKIVRSLWKESNDTVRLNDVDGMYPCHDGEGVYRYVIEGDVMTWVM
                     LSDPCPDRAGALNNTKFYRKK"
     gene            2004..2786
                     /locus_tag="VGQ51_11785"
     CDS             2004..2786
                     /locus_tag="VGQ51_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9037300.1"
                     /translation="MNNTSQQPVLQVQGLDIFYGNFQAVKKVSFAVMPGEIFGLLGPN
                     GAGKTSTLSAVESLIKPSSGSITVAGYDTQRQTLYARACMGVQLQSTSFQPELTVSEI
                     LQLYGGIYGITLDRQRLDEILRDIKLQDAASKRFGQLSGGQQQRVSLVIATIHNPPLL
                     LMDEPTTGLDPQSRRQLWVRIEAMRDKGHGILLTTHSMEEAESVCDRIAIIDHGRIIT
                     IDTPQNIIDTNRDRPEVVQASRKGKITLEDVFIGLTGEAIRS"
     gene            2816..3601
                     /locus_tag="VGQ51_11790"
     CDS             2816..3601
                     /locus_tag="VGQ51_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037301.1"
                     /translation="MSNTTTAPITLTNPRTLSSTRSVFASLLRADLTTQWRNRRATVM
                     VFILPVIILFAWKPLIKAFGGPFILANCITIGLNAVGIMGYSNSIARDRDKGIFQRLR
                     VSPAPNWTIMVSRLTVQLVLISIMTLFVFIGGYQYDHIALTAGGYVASFFTSLACGAV
                     YLALGQAIVGRITNPETVNATSRLIYLALILIGMFGSLGMLGNAMNKIVDYSPYGTVK
                     TIVTHALVPSTWNADANLALLITLGYTVVFAYLGIKWFKWNSR"
     gene            complement(3659..5746)
                     /locus_tag="VGQ51_11795"
     CDS             complement(3659..5746)
                     /locus_tag="VGQ51_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037302.1"
                     /translation="MRKTTLSVGLFLFVGAAWAQDSSNLNIGYLSLRQEFTQTIRVKG
                     ADLEKMPFVNLSDAIAQWFFGAYTQPASLIYVVDGNPVTDVNSYSVYDIDEVILVQNA
                     AALATTASGQQEMVFVRTKRGGGKSGLTAAVQSGLVNVSGYHPKAGWFQNIYAGGYRN
                     WNKVRAGLSVNYMRDVFPDTVGRHEAVTQKNLQRWRLRGYVDWQIGHRNLVELTVNYT
                     PQRIGAGGDSIVQPSDNRRSDMATQHLVVPHLFWHSDLGGGWRNEFQATYLHGSYREN
                     DYLLNYTGSPQYGDYFDSTLYHQTSYHLFLWDRMEHRGKIGGVDIVPAVNASYEYARE
                     VSAGLSYSGDEPGTLGTGQRAIYSISYDSNWQSGKFLFITPSLDISYKRAFDVQGGVL
                     IEAGHQHGPKNQQGFAFVTVTLDPVKMVNEQSRSSLRLFGSYARRTVATTSGYTLADL
                     DHGPVAAALGGLQVQANGIGSLIGLGSGGFFIPAVLRVPDFGVGQAGLSLSTWNDRLL
                     VQYNFERRNYLTGVAEFVFYYSGNAYTILFPTMDEVLHHADVSVILIKHGGFEWRSRL
                     NMTMMKSTLNLNVGNAQVTAIGDVAPARWSQTGGWVNRIVVNGFSAGLDVLYHFGETY
                     YRPSYNGSLSVKAVGKRSSVLVPNVYAGYEWKTARSGAVEVFLESRGAIRNSPNDLSD
                     GRRWYTVGGKWSL"
     gene            complement(5793..>6622)
                     /locus_tag="VGQ51_11800"
     CDS             complement(5793..>6622)
                     /locus_tag="VGQ51_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037303.1"
                     /translation="LDLLHTGSGSGGSSLKLFGSYARRPLLYVNEYSLNDLSSTGKYY
                     GNYSLQQLYWTMSGYTFYNGTGVTYVPFAEDEPGYWAWVTGAAYKSADGRVSLQYSLE
                     RRNFSLTVPTNSGSPGFEPWVETLQNADLRIRVIESKKASWMTGFNVSLMHNNGDTAG
                     YQQATVGGPIGDLNGQGNSWTGGWVNRLRLGAFSAGLDLLYHFGETVPVFSFSGAPIG
                     EARKNSVLLPNIYAGYRWSLGAGVLELFVGGRGLLGTRDSDLPDTRRFYTIGGSYSL"
BASE COUNT         1581 a         2142 c         1487 g         1412 t
ORIGIN      
        1 cgcgtatcct gagcgacaga caacgactcc ggcagatact gatcaatctg gtcggcaatg
       61 ccgttaagtt tacggaaaag ggcgagatcc gcgttcgtgt attcctgccc cccacatctt
      121 ccatgcccga tattccggca ggacatatcc agatcgcttt cgagatacag gacacaggca
      181 tcggcatcct tcccggtcag ctcaaccgcc tcttccaatc cttttcgcag gtggactcct
      241 cggtcacgcg gaaatatggc ggcatcggtc tgggtctctc catctccaac aagctcgtcc
      301 atctgatgaa cggccagata acggcggaaa gcgcggccgg cgttggctct gttttcatgt
      361 tcaccatcac tacgcgcccg gcgccgctcc gctcgtccaa tcctgccgca gtcgccatcc
      421 ccgatctggc ggctaaatat cccctgcgca tcctgctggc cgaagacaac cccatcaacc
      481 agcagctggc gatgatcaca ctgaacaaaa tgggctattc acccgccgtc gccgcaaatg
      541 gccgggaggc cgtcgagcgg ctcaaacagg aatccttcga tctgattttc atggatgtcc
      601 aaatgcccga aatggacggc ctggaggcta cccgccacat tcgccaaaaa ggagacagac
      661 aaacggtcat catcgcgatg accgccaacg cgacgatcca ggatcgcgat gattgtcttg
      721 ccgaaggtat gaacgactat ctatgcaaac cggtcgacct gggcgaactg atcttcacgc
      781 tcgagaagtg gggccaccag atcaataaaa aagctgccag ctgacccatg cggtccccct
      841 ccaaaaaaag ggttggttat catgaccgga ccccacctaa aatacccatt ttcaggtagt
      901 tattatgact accggcccgg ttggcgtaat agaaaccgac caaactgtgt ttttcttccc
      961 ccgtatactc tactacattt gtggaacaat tcgcgattgc tacgccgaaa cccgtaaaag
     1021 taaaatcacc atagttatga aagcagtagc aagaatcatc gcagtagcct taagcacgat
     1081 cgttatcctc gttagcaacc ccctggcaag ccaggccaat ggcggccgca ccgatagcaa
     1141 gaaggccctc accgaagaaa aagtatccat taaatatgta ggctccgaga acggacaggt
     1201 cgtcttccgc gtggaattcg aaaaccctac ctcggagaag ttctccctga tcatccggaa
     1261 tgacaatggg gacgtggttt atcaccagca gttcaatgat acgcatttct cgaagaatgt
     1321 cttcatcgcc aatgtcgact ccgacatcca gccgacgttc gtcatccgca gcgccgacaa
     1381 ttccgagatc gttcgtcagt tccgtgtatc taagaccgtt actgaaaaca cgaccgtaac
     1441 tcgtctttaa tttcttaaat tcaaacctcg gggtccggct tgcgccggac cctttcttat
     1501 ttctttctat agaacttcgt gttgttcaat gcccccgccc tgtccggaca gggatccgac
     1561 aacatgaccc aggtcatgac gtctccctcg atgacatacc ggtaaacgcc ttccccgtca
     1621 tgacagggat acataccgtc gacatcgttg agccgcacgg tgtcgttcga ttccttccac
     1681 aaggaccgca cgattttgtc cccggtggtg gatgccgccc aactcgtgtc ggcgtcgaaa
     1741 tgataggtca gcgtatcgtt caacccctga acatagaatt tccattcgga attcttcaac
     1801 gattgggccc tggactggat aaatccgccc agactgagaa ataataggat cgcgagtgtt
     1861 ttccgcataa aatgttttta gttccctaat atattgaatt atttccgcgt ttcaccgcca
     1921 tggcccgctc gtttaccggc tttttccccg gtgatggccg ccccggcctt gccggggcgg
     1981 ccacaacttt ttatcttcgt gccatgaata atacatccca acagcccgtc ctccaggtcc
     2041 agggcctgga tatcttttac ggcaacttcc aggccgtcaa aaaagtctcc tttgcggtga
     2101 tgcccgggga aatattcgga ctgctgggcc ccaacggagc cggcaagacc agcaccctca
     2161 gcgcggtgga aagcctgatc aaaccctcct cgggaagcat taccgtggcg ggctatgaca
     2221 cccagcgtca gacgctctac gcccgggcct gtatgggcgt acagctccaa tcgaccagct
     2281 ttcagcccga actcaccgta tcggagatcc tgcagctata cggtggcatc tatggtataa
     2341 ccctcgaccg ccagcgcctc gacgagatcc tccgcgacat caagttgcag gacgccgctt
     2401 ccaaacgctt cggccagctt tccggcggtc agcagcaacg cgtctctctt gtcatcgcta
     2461 ccattcataa tccacccctc ctgctcatgg acgaaccgac caccggtctc gacccgcagt
     2521 cgcggcgtca gctctgggta aggatcgagg ccatgcggga caaggggcac ggcatcctgc
     2581 tcacgaccca ctcgatggaa gaggccgaat ccgtctgcga ccggatcgcg atcatcgatc
     2641 acggcaggat catcaccatc gatacaccac agaacatcat cgataccaac cgcgaccgtc
     2701 ccgaggtcgt ccaggcatcc cgtaaaggaa aaataacact ggaagacgtc ttcatcggcc
     2761 ttaccggcga agcgatccgc tcctgaattt ataccaccaa ctaaaaaacc ggatcatgtc
     2821 gaatacaacc accgctccca tcaccctgac gaaccccagg accctctcct ccacgcgaag
     2881 cgtcttcgcc tcgctgctcc gcgccgatct gaccacccaa tggcgcaaca gacgcgcgac
     2941 cgtcatggta ttcatactgc cggtcatcat cctcttcgcc tggaaacccc ttatcaaggc
     3001 tttcggcggc cctttcattc tcgccaattg cattaccatc gggctcaacg ccgtcggtat
     3061 catgggctat tccaattcca tcgcacgcga ccgcgacaaa ggtattttcc aacggctacg
     3121 ggtatcgccc gcccccaact ggaccatcat ggtcagccgt ctcaccgtcc agttggttct
     3181 gatcagcatc atgacgctct tcgtatttat cggtggatat caatatgacc atattgcact
     3241 cacagccgga ggttacgttg ccagcttttt cacctccctg gcatgcggcg cggtctatct
     3301 cgctcttggc caggccatcg tcggccggat cacgaacccc gagacggtca atgccaccag
     3361 ccgcctcatt tacctcgcac tcattttgat cggcatgttc ggttcgctgg gtatgctggg
     3421 gaatgccatg aataagatcg tcgactattc gccctatggc acggttaaaa cgatcgtaac
     3481 gcacgcgctc gtcccctcaa cctggaatgc cgacgccaat ctcgcactac tcatcacgct
     3541 tggctatacg gtcgtattcg cgtatctggg tatcaaatgg ttcaaatgga atagccgcta
     3601 acgcgatgcc cgctaacgcg ccacccgcgg acccgccacc ccgccgacac cgcatcccct
     3661 acaacgacca tttacccccg acggtatacc accggcgccc gtccgacaga tcattcggag
     3721 agttgcggat cgccccgcgg ctctccaaaa atacctccac cgcacccgac cgcgccgtct
     3781 tccattcata acccgcatag acattcggaa caagcaccga actacgcttc cccaccgcct
     3841 ttacagaaag actgccattg tacgacggcc tgtaatacgt ctcgccaaaa tgatagagca
     3901 catcgagccc cgccgaaaat ccattcacca cgatccggtt cacccatcca ccggtctgtg
     3961 accagcgggc cggcgccaca tccccgatcg ccgtcacctg cgcattcccc acattgagat
     4021 tcaaggtact cttcatcatg gtcatattca accgggacct ccattcgaag cccccgtgtt
     4081 ttatcagaat cacgctgaca tcggcatgat gcaggacctc gtccatggtg gggaaaagga
     4141 tcgtatacgc attcccgcta taatagaaca cgaactccgc tacacccgtc agataattcc
     4201 tccgttcaaa attgtactgg accagcagcc ggtcattcca cgttgacagg ctgagccccg
     4261 cctggcctac tccgaaatca ggcacacgca acaccgccgg aataaaaaag cctccgcttc
     4321 ccaacccgat cagcgacccg atgccattgg cctgcacctg gagcccacct aacgcggccg
     4381 ctaccggtcc atggtccaaa tccgctaacg tatacccgct ggtcgtcgct accgtcctgc
     4441 gtgcataaga accgaacaac cgcagactgg accggctctg ctcattcacc attttcacgg
     4501 gatctagggt cacggtcaca aaggcaaacc cctgctgatt tttcggacca tgctgatgac
     4561 cggcttcgat caacaccccg ccctgcacat cgaatgcacg cttatagctg atgtccaacg
     4621 acggcgttat aaaaaggaat ttgccggact gccaattgct gtcataggag atggaataaa
     4681 tggcccgctg gccagtcccg agagttccgg gctcatcacc cgaatagctt aaccccgcac
     4741 ttacttccct cgcatattca taactggcat tcaccgccgg cacaatatcg acgccaccga
     4801 tctttccccg atgttccatc ctgtcccaaa gaaaaagatg ataggaagtc tgatgataca
     4861 gggtcgaatc gaaataatcg ccgtactgag gcgaccccgt atagttaagc aggtagtcgt
     4921 tctcacggta gcttccatgc agataagtcg cctggaattc attcctccaa ccaccgccca
     4981 ggtccgaatg ccagaaaaga tgcggaacca ccaggtgctg agtggccata tcggaacgcc
     5041 ggttatccga aggctgtacg atagaatctc cacccgcccc tattcgttgc ggcgtataat
     5101 tcacagtaag ctccaccagg ttccgatgac cgatctgcca gtcgacatat ccccgaagcc
     5161 gccagcgttg caggttcttc tgcgtcaccg cctcatgccg tcctaccgta tccgggaaga
     5221 catcacgcat atagttcacc gataacccgg cgcgcacttt attccaattg cgataacctc
     5281 ccgcataaat attctgaaac caccccgcct tcggatgata cccgctcaca ttgacaagcc
     5341 cgctctgcac cgctgccgtc agccccgatt tcccgcctcc tcgtttcgtc cggacgaaca
     5401 ccatctcctg ctgacccgat gcggtggtcg ccaacgcagc agcattttgc accaatatca
     5461 cctcatcgat atcataaacg ctgtaggaat tgacgtccgt caccggattt ccatccacga
     5521 cataaatcaa acttgctggt tgcgtatacg ccccaaaaaa ccactgggca atggcgtcgc
     5581 tcagattgac aaacggcatt ttttcgagat cggcgccttt gacacggatc gtctgcgtaa
     5641 actcctgccg cagcgacaga tatccgatat tcaaattgga agaatcctgg gcccatgccg
     5701 caccaacaaa aaggaacaga cccaccgata aggttgtctt tctcatacgc aggttgttag
     5761 atcaacccaa tataatacga atgctccact tctcaaagcg aatagcttcc cccgatagta
     5821 tagaacctcc gcgtgtccgg cagatcgctg tccctggtcc ccaacagccc cctgcccccg
     5881 acgaacagct ccaatactcc ggctcccagg ctccagcggt atccggcata aatattcggc
     5941 agcaacacgg aattctttct ggcttccccg atcggcgcgc cggaaaaaga aaacaccggc
     6001 accgtctcac cgaaatgata tagcagatcc agtccggctg agaacgcacc caaccgcaac
     6061 cggttgaccc aaccgcccgt ccacgaatta ccctgtccat tcaggtctcc gatcggtccg
     6121 cccacggtag cctgctgata tcccgccgta tcgccattat tatgcatcag gctgacattg
     6181 aagcccgtca tccagcttgc ttttttcgat tcgatcaccc tgatccgcag atccgcattc
     6241 tgcagtgtct ccacccaggg ttcaaaaccc gggctgccac tattcgtagg cactgtaagc
     6301 gaaaaatttc gccgctccag actgtattga agggagaccc gtccatccgc actcttatat
     6361 gccgccccgg tcacccaggc ccaataaccg ggttcgtcct ccgcaaacgg tacatacgta
     6421 actccggtcc cattataaaa cgtataaccg gacatggtcc aatacagctg ttgcaggcta
     6481 taattgccat aatacttgcc cgtagaggaa agatcgttca aactgtattc gttgacataa
     6541 agaagcggtc gacgggcata ggaaccaaac aacttcagac tgctgccgcc cgatcccgaa
     6601 cccgtatgca acaggtctag cc
//
LOCUS       DASYSV010000384         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2121560, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000384 DASYSV010000000
VERSION     DASYSV010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121560"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VGQ51_11805"
     CDS             complement(<1..429)
                     /locus_tag="VGQ51_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037304.1"
                     /translation="MEELLGFLNQIASSEGWPLSAGCLAHLRKIIKIRTLKQEELLLS
                     VGQVCKNIYFINRGLLKCFYIVHQQEVSDWFFGELETVVSIDSFYDQVASEDFIEALE
                     HCELFCITFEELNFLFRAFVEFNVVGRVLTNKYLRIWHRQA"
     gene            complement(619..2832)
                     /locus_tag="VGQ51_11810"
     CDS             complement(619..2832)
                     /locus_tag="VGQ51_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEV9037305.1"
                     /translation="MSRRILLFFTVLALAACKGRSHPNQEMIDLLKVSAKFDHNHENV
                     FSPEAMIEYCDSVLGAGPGEDARRDALSRKANALLELGEEQKAIDIYEGILGKIPPGN
                     PDQRQAILKDMALAWLRLGDRVNCINNHTAESCIYPIAGGGIHRDKTGAEKAIDIYKT
                     ILVESPSDLESRWLLNIAYMTAGGYPSGVPPQFLLKVRDDDSMHLVKPFRNVAMNAGL
                     DYKCIAGGSIVDDFNNDGYDDIILSSWSLTEPMRYYRNNADGSFTDVSDSSGLGYLTG
                     GLNIQQMDYNNDGYKDIFVLRGGWKGKFGKNPNSLLRNNGDGTFTDVTKASGLLSFHP
                     SQTATWADFNNDGWLDVFIGNETTPNEEPHPCQLFINNKNGTFTEIAARAGCAVTQFV
                     KGVTSGDYDNDGLPDIFLSTLNGNKILLRNETGKNGEVKFRDVSEQAGLSHNLTRTFP
                     TWFWDYDNDGWLDILVCGYEFNRSLSYYAGAEAVQLPIGNAGKVFLFRNKHDGTFEEV
                     SAKVGLSSTAFAMGANFGDIDNDGYLDFYLGTGNPQLRSAVPNKLFKNMGGQRFLDVT
                     TAARVGNIQKGHGVSFADLDNDGNEDIYIKMGGAYTGDAFENCLYLNPGQNNNHWVNL
                     QLQGTVSNKAAIGARIKVTFLENSKERSVFRDVNSGGSFGSNPLRQHIGIGKAAAIGR
                     VEINWPVTGKTQVFRNVPIDTNVKIVEGDSVFTTYRLARLDFRSGVRKLISCSPK"
     gene            complement(2837..>3538)
                     /locus_tag="VGQ51_11815"
     CDS             complement(2837..>3538)
                     /locus_tag="VGQ51_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037306.1"
                     /translation="DGDLDLVAGNLGLNCDYHVGPGTPMELFADDIDQNGRIDPVMFY
                     YLKDADGVRRSYPAYSRSQLAAQVPTVKKQFLLNDDYARAGFSELFPGKTKADVLNFY
                     CDETRTCWLENDGNGKFIKHVLPMEAQFAPVNTIICADVDNDGYKDLLLAGNDYQTDV
                     ITGRYDASYGCLLRGSGKKRFTAVPASRSGFVLKGDVKDMSLIRLANGERIVLASVND
                     DSLRVFRINEQNLKN"
BASE COUNT          854 a          997 c          812 g          875 t
ORIGIN      
        1 agcctgccgg tgccatattc tcaaatactt attggtcagc acccgaccaa cgacattaaa
       61 ttcaacgaaa gctcgaaaga ggaaattcaa ttcctcgaac gtgatacaaa atagttcaca
      121 gtgttccaaa gcctcaatga agtcctcgct tgccacttgg tcataaaagc tgtcaatgga
      181 aacaaccgtt tccaactccc caaaaaacca gtcggaaact tcctgctgat gtacgatgta
      241 aaagcacttc agcaagcccc tattgataaa atatatattt ttacatactt gcccgacaga
      301 caacaaaagc tcctcttgtt tcaaagtgcg gatctttatg atcttgcgaa gatgtgcaag
      361 acaaccggcc gacagcggcc atccctccga ggaggcgatt tggttcagga aacccaggag
      421 ctcttccatg gcttgacaag gtttaatggt tactaccaat ttaacaatct tccgcgacag
      481 tccttcgagc tcatatagtc gcgccgtaag tcataaacgt gttatctcgg ctcagcgact
      541 cactaaaagg gaggcatccc cctttcacct acccgatgtt tcccctgctc cgccgtagcg
      601 gacagcagtc accatcacct acttcggcga acagctgatc agctttctca cacccgaccg
      661 gaagtccaac cgggcaagcc gatacgtcgt aaacacgcta tccccttcga cgatcttcac
      721 attcgtatcg atcggtacgt tccggaacac ctgcgttttc cccgtgacgg gccagttaat
      781 ttcaacccgc ccgatcgctg ccgccttccc gatgccgata tgctgacgca aagggttgga
      841 accaaaactt ccgccggagt tcacatcgcg gaagacggat cgttctttac tgttttcgag
      901 gaacgtaacc ttgatcctgg ccccgatggc ggccttattc gacacggtac cctgcaactg
      961 gagatttacc cagtgattat tattctgccc cggattcagg taaagacaat tctcaaaggc
     1021 gtccccagta taggcgccgc ccattttgat ataaatatct tcattgccat cattgtccag
     1081 gtccgcgaac gaaacgccat gccccttttg tatatttcct acccgggctg cggtcgtgac
     1141 atctaaaaac cgctgccctc ccatattctt gaacagcttg ttagggacgg cggacctaag
     1201 ctgagggttg ccggtcccga gataaaaatc gagatatcca tcattatcga tatcgccaaa
     1261 attggcgccc atggcaaagg cggtggaact caacccgact tttgcagaca cttcttcaaa
     1321 agtgccgtca tgcttatttc ggaataagaa aacctttcca gcgtttccaa tcggcaactg
     1381 taccgcctcg gcccctgcat aataggagag cgaccggtta aactcatacc cgcacacaag
     1441 gatatcgagc cagccgtcgt tgtcgtaatc ccagaaccag gtcggaaagg ttcgggtgag
     1501 attatgcgat agcccggcct gttcgcttac gtccctgaac tttacctcac cgttttttcc
     1561 tgtttcgttc ctcaacagga tcttgttgcc atttaatgtg gagagaaaaa tatccggtaa
     1621 cccgtcgttg tcatagtcgc cggaggtgac gcctttcaca aactgcgtaa cggcgcagcc
     1681 ggccctggcg gcaatctcgg taaaagtccc gttcttatta ttgataaaca gttgacaggg
     1741 atgcggctct tcgttggggg tcgtttcatt tcctataaat acgtcgagcc agccgtcatt
     1801 gttaaagtcc gcccaggtgg cggtttgcga ggggtggaag gacaacaacc cgctggcctt
     1861 cgtgacgtcc gtaaatgtcc catccccatt gttccgtaac aaggaatttg ggttcttgcc
     1921 gaacttgccc ttccagcctc cccgcagaac aaaaatatcc ttgtagccgt cattattgta
     1981 atccatctgc tggatattga gtcctcctgt gagatagccg agacccgagg aatccgaaac
     2041 atccgtaaag ctcccgtcgg cattattccg gtaataacgc ataggctcgg tcaaagacca
     2101 gctactgaga ataatatcgt catagccgtc gttgttgaaa tcatccacga tcgacccgcc
     2161 ggcaatacat ttgtaatcca ggccggcatt catcgccaca tttctaaaag gctttaccag
     2221 gtgcatacta tcgtcatccc ggacttttag caagaattga ggcggaaccc cggatggata
     2281 tcctcctgcg gtcatatagg cgatgttgag aagccatctg gactcgagat cggaagggct
     2341 ttccaccagt atggttttgt aaatatcgat ggccttttcg gccccggtct tatcgcgatg
     2401 tatcccgccg ccggcaatcg ggtaaataca agactccgcg gtgtggttgt tgatgcagtt
     2461 gaccctatcg ccgagcctta gccaggcgag cgccatatcc ttcaggatcg cctgtctttg
     2521 atccggattg ccgggcggga tcttcccaag tattccttcg taaatatcga tggccttctg
     2581 ctcttcgccg agttccaaca aagcattcgc tttccggcta agcgcatccc gccgcgcgtc
     2641 ttctccggga ccagcgccca aaaccgaatc gcaatattct atcatagcct cgggtgaaaa
     2701 cacattctca tggttgtggt cgaatttggc cgatactttc aacagatcga tcatttcctg
     2761 gttgggatgc gatctaccct tgcaggccgc aagggccagt acagtgaaaa acaacagtat
     2821 gcgacgcgac atatctttaa tttttcaaat tttgttcgtt gatgcggaac acccgcaagc
     2881 tatcgtcatt gacggaggca agaacgatcc gctccccatt ggcaagccgg atcagcgaca
     2941 tatcttttac atcccctttc agcacgaacc cgctgcgcga tgccggcacg gcggtaaatc
     3001 ttttcttacc gctgccgcgc aacagacagc cataagaagc atcgtatctg ccggtgatga
     3061 cgtccgtctg ataatcattc cccgccagca ggagatcctt atatccgtca ttgtcgacat
     3121 ccgcgcaaat gatcgtattg acgggcgcga attgcgcttc catcggtagc acgtgtttga
     3181 taaactttcc gttcccatca ttttcaagcc agcaagtcct ggtctcatcg cagtagaaat
     3241 tcagcacgtc ggctttcgtc ttacccggaa aaagctcgga aaagccagcc ctggcataat
     3301 cgtcgttcag gagaaattgc tttttgactg tcggcacctg tgcggccagc tggctcctgc
     3361 tatatgcggg atacgagcgc ctgacgccgt cggcgtcctt taagtaatag aacatcaccg
     3421 ggtcgatcct gccgttctga tcgatgtcgt ccgcaaacaa ttccatgggt gtcccgggac
     3481 cgacgtgata gtcgcagttg agcccaaggt tacccgcaac aagatcgagg tcgccatc
//
LOCUS       DASYSV010000385        10509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_975467, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000385 DASYSV010000000
VERSION     DASYSV010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10509
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975467"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VGQ51_11820"
     CDS             <1..590
                     /locus_tag="VGQ51_11820"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEV9037307.1"
                     /translation="LEMAVKDAHTWLEYNYDRLPPFYEGTHWFFPATEETHQSILQDF
                     RVPDSYPIDNRGTLYMIAFFSAKHIGESQYYLMTTSDKDGHPLDGNVSYRLHVPANVP
                     ITQFWSMTVYDRDTHTFIRNARWMDRSSQTPGLRTNADGSVDIYFGPTAPASGEANWV
                     PTDAHGGFEVLARFYEPKKALYDKSWSMGDLEKQR"
     gene            complement(642..1412)
                     /gene="xth"
                     /locus_tag="VGQ51_11825"
     CDS             complement(642..1412)
                     /gene="xth"
                     /locus_tag="VGQ51_11825"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV9037308.1"
                     /translation="MKIATYNINGVNGHLPVLVRWLRETGPDIVCLQELKAPQEKFPE
                     DAIREAGYHALWHGQKSWNGVAILSRDGLPEEVCRALPGDPEDLHSRYIEAMVGGLTI
                     GCLYLPNGNPAPGPKFDYKLQWFQRLTTHAAALLASGRPVILTGDYNVMPTELDVYKP
                     ESWVDDALFRPETRLAFKTLVGQGWTDAIRKLYPEEKIYTFWDYFRNAFGRNAGLRID
                     HFLLSPSVAPRLIAAGVDKEVRGWEKSSDHAPVWIQLK"
     gene            1528..2829
                     /locus_tag="VGQ51_11830"
     CDS             1528..2829
                     /locus_tag="VGQ51_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV9037309.1"
                     /translation="MHGSIFAKVFLILIAVDLLLDCYTFMGIRTLTNTWKSAGLRQVV
                     NWGWLGVVVGITVLLVAGFDSFRHPSGMMPYREWVLSLFLALFVTKLFFAIILLLGDI
                     GRLLYGLVKRLLRPADAVADGSRSMAIGTATGEPLIPARRKFISELGVLVAAVPFSSF
                     GYAMLRGKYDFRLHKIALHFRDLPEAFDGFTITQVSDIHSGSFDNVRAVQRGIDLAKA
                     QNSDLFVFTGDLVNNVAWEIEPYIDRFAALKAPYGQYSILGNHDYGDYIKWDSLDDKA
                     RNLEALKRHHRDLGYRLLMNEHVLLEKDGQRIALIGVQNWGRGFIQLGDLDKALEGLG
                     NDVFKICLSHDPTHWEEKIRFHPTTIHLTLSGHTHGAQFGVESDQWRWSPVQYRYLDW
                     AGLANERDRYLYVNRGFGFLAFSGRLGIWPEVTVITLKRGV"
     gene            complement(2807..3337)
                     /locus_tag="VGQ51_11835"
     CDS             complement(2807..3337)
                     /locus_tag="VGQ51_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9037310.1"
                     /translation="MTITPTEPGDLADIETLFTLAIEYQRSKGSYSWQGMDRPLIERE
                     IAERLHWKIIGDGVIACFFSIAFTDRVVWDERDVDPSIYLHRIVTNPLFRGAGYVRHI
                     VAWAEDYGRSMGKDYIRLDTGRDNQRLNAYYRECGFEYCGVKQFPDDADPAVPRHYLG
                     SGISLFERRIRPLVSM"
     gene            complement(3377..4081)
                     /locus_tag="VGQ51_11840"
     CDS             complement(3377..4081)
                     /locus_tag="VGQ51_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEV9037311.1"
                     /translation="MLKVPVYLTLVIGLPTAANTGQPASQQANAAPPATAYVSLTAAK
                     SVGAIALPQNRSLKFVDAYIHDNDEDLALVKQHSKRSFTIIDSVLVHYGLPTDLKYLA
                     VIESNLKTSAVSRVGARGAWQLMAGTARDLGLKVNGRYDERTNLYKSTRAAALYLRDL
                     HSQFKDWLLVLAAYNAGPLPVIRAIHRSGSHNFWALERYLPKESRAHVKHFIATAIYF
                     QHYEEINAASQALAAL"
     gene            4293..6263
                     /locus_tag="VGQ51_11845"
     CDS             4293..6263
                     /locus_tag="VGQ51_11845"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEV9037312.1"
                     /translation="MRKPLLFLACLFNMLITNAAPAPRDYLVHSPDGSLTVRISVDEH
                     TRWSAEMDGQPVITPSAISLRLSDGVILGDNMKVVSAPVQTVDVNFDPVNYKKSHIRD
                     HYNQLTLNVKGDYSVVFRVYDDAVAYRFVTRKQGELIVRDEGAEFNFAADDDAFIPIQ
                     WDYRAGKNFNSSFEALYHEIHLSQMPKDSLAFLPLLVDLGQNKKAVILEADLQDYPGM
                     YLGANGNGLKGVYAPYPLDAALHSINYIPGTRADYIARTTGTRNFPWRVVVVSRQDKD
                     LLNQDIVQKLAEPSQLKDIGWVRPGQVAWDWWNNWNVSHVDFKAGINTPTYKYYIDFA
                     AANHIPYIIMDEGWSNDLDLMQQSSRIDLKEIIDYGKQHGVGVILWATWYAVTHQMEK
                     AFPFYANMGVKGFKIDFIDRDDQVAIASTYAIARLAAANNLMIDYHGTSKPAGLQRTW
                     PNVIGYEGVKGLENFKWANEDQPRYVATIPYIRMMAGPMDYTPGAMRNATQSAYRPIN
                     DNPMSKGTRCQQLAEYIVFEAPLQMLSDNPTTYRKEQECTDFITKVPTTFDETTALDG
                     KVGEYVAIARRRGDTWFLGAITDWTPRQLTIDFSFLPAGHYEAEVFQDGVNADRDATD
                     YRRQVISVKSGDKLSVQLYGGGGWVARIYPVK"
     gene            complement(6266..7078)
                     /locus_tag="VGQ51_11850"
     CDS             complement(6266..7078)
                     /locus_tag="VGQ51_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEV9037313.1"
                     /translation="MLKSPLPFYVCSIAFFVGVPSDANPGQPYRDTAKDVAHAGGKHR
                     TAVAKKQLISVPIPEKGMKFVSSYIRNNDDCLTSVRQRSDGPFAIIDSVLDHYGLPQQ
                     LKYLAVIESELKPTATSRVGAKGPWQLMRSTAHVLGLKVNRRSDERKDYYKSTRAAAK
                     YLRDLHREFGDWLLVLAAYNGGPRPVCRAIRKSHSRNFWVLQRYLPAESRQHVKKFIA
                     TAYYFEHSANAAATTPTPSAILQEKDNDKFTRLMSESAASLQASNELLVQPN"
     gene            7323..7682
                     /locus_tag="VGQ51_11855"
     CDS             7323..7682
                     /locus_tag="VGQ51_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9037314.1"
                     /translation="MTPSIGNPVVHFEIGCKDLEGTTSFYTKLFGWTASTVPTAAILE
                     THSTNGIPGHITSLGHEPHQYVTFYIEVDDIDETLGRIAGAGGKKVIGPFPLPDGKKF
                     AWFSDPGGNMIGLITKT"
     gene            complement(7679..8101)
                     /locus_tag="VGQ51_11860"
     CDS             complement(7679..8101)
                     /locus_tag="VGQ51_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037315.1"
                     /translation="MSKKVLFYCAKVWLTSLVLGPMLYFAWSWSNLEGLLNFYGFTIA
                     AILSGFILSLPSALFFWGGAACLRTSQLSVPTQRLLLCAWTALLTFVPFWLLYDWAGH
                     NPGSSLEQWLQLPLCCWVILVIGIYTFRFPTKVTGRAS"
     gene            8271..9023
                     /locus_tag="VGQ51_11865"
     CDS             8271..9023
                     /locus_tag="VGQ51_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV9037316.1"
                     /translation="MATSEHILTIDVGGSHIKGTILDSEGNQQMKYKKLVTPANATPH
                     AVLTTILSLVEGMPEYGRVSVGFPGYVRNGIVYTAPNLQNLLWKHIEFGQQISDALQK
                     PVRLVNDADQLGLGVVSGEGFELAVTLGTGFGTAVLIDGYLLPHLELAHHPLHKGKDY
                     DAYIGEKAMKKAGRKKWNQRVKIVLEILKTVFNYDRLYLGGGNASKLTIPLDGNIHLF
                     TNEDGIRGGARLWKMEDKYHITTNYPKPGGTA"
     gene            9020..10063
                     /locus_tag="VGQ51_11870"
     CDS             9020..10063
                     /locus_tag="VGQ51_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEV9037317.1"
                     /translation="MNKPESERLKEPGLKLSTFFEMTPDLVCVAGRDGYFKKVNRAVL
                     EAFGYTEQELMSRPISSFMHPEDRELTLHKRNELFDGKALVNFQNRYVTKTGAIIWLH
                     WTSIYLEDSGVVFAIAKDITEQKKIELDIEAKYKKFKGLAAHFKTSMEEDRKYLAREL
                     HEELAQLASVLKMDIEWVKNHSPELSPASKDRIEHAAVISQMLITTIRRLSFYISPNM
                     LEHIGLTATMEWYCREFSILTGIPCRFESEFNEKGLTEEIRIDLFRICQEALMNTLNH
                     AQANHVTVRIEDRVRQLQLTVTDDGQGFDVAKQQRKSGMTRMRERAASINAQLTIDSA
                     PGKGTTVAVTISK"
     gene            10137..>10509
                     /locus_tag="VGQ51_11875"
     CDS             10137..>10509
                     /locus_tag="VGQ51_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037318.1"
                     /translation="MRPSISMKALAGVLFLFVFCHSPVAGQMRQIYLDPDSTNHINRL
                     SFYSPNEGYVAFEHWIGYTTDSGRSYTKIYITPNNVNFNGYSSANWVVGFFINGVKAI
                     SKNILIVYGNYDLVPSILYSSD"
BASE COUNT         2485 a         2736 c         2909 g         2379 t
ORIGIN      
        1 tccttgaaat ggccgtaaaa gatgcgcaca cctggctgga atacaattat gacaggctgc
       61 ctccatttta tgaaggaact cattggttct tccccgccac ggaggaaacg catcagagta
      121 tactgcagga tttccgggtg ccggattcct atcccatcga taacaggggc accctctata
      181 tgatcgcttt tttcagcgcc aaacatatcg gcgaatcgca atattatctc atgactacca
      241 gcgacaaaga cggacatccg ctggacggta atgtctctta tcgcctgcat gtgcctgcta
      301 atgtaccgat cacgcaattc tggtcgatga cggtatacga tcgggatacc cataccttta
      361 tccgcaatgc ccgatggatg gaccgctcat cgcaaacgcc cggtttgcga accaacgccg
      421 acggttcagt cgatatctat ttcggaccga cggcgccggc ttcaggggaa gccaactggg
      481 ttccgacaga tgcgcatggc ggttttgaag tgctggcgcg tttctatgaa ccgaaaaagg
      541 ctttatacga taagagctgg agtatgggcg atctggagaa gcagcgctga accgctgacc
      601 ggcgcatcgc ctggaaccgc tgaccgcccg accggcgccc cctatttcaa ctgtatccac
      661 accggcgcat gatcgctcga tttctcccag ccgcgcactt ccttgtccac gcctgccgca
      721 atcagccggg gggcgaccga cgggctcagc aggaaatgat cgatccgtag tcctgcattg
      781 cgcccgaagg cattgcggaa ataatcccaa aatgtataga tcttctcttc cgggtacaat
      841 ttccgtatcg catcggtcca gccctggccg accagggtct taaaagccaa gcgcgtctct
      901 ggccggaata acgcatcatc gacccaactc tccggtttat agacatcgag ctcggtgggc
      961 atgacattgt aatcgccggt caggatcacg ggccggcccg aggccagcag tgccgccgca
     1021 tgagtggtaa ggcgctggaa ccattgcagc ttatagtcga atttcggacc gggtgcagga
     1081 ttgccattgg gtaaataaag acaaccgatg gtcagcccgc ccaccatggc ctcgatataa
     1141 cggctatgaa gatcttccgg gtcgcccggt agcgcgcggc atacttcctc cggcagacca
     1201 tcccgtgaaa ggatcgccac gccattccag cttttttgtc catgccacag cgcatgatag
     1261 ccggcctcgc gtatggcgtc ttcggggaac ttttcctggg gcgccttcaa ttcctgcaga
     1321 caaacgatat ccggccccgt ctcgcggagc catcgcacca aaacgggcag gtgtccgttg
     1381 acgccattga tattataggt ggctattttc atgatttcga actttgagcc gccaatggcg
     1441 gctcgtccga aggacagaca ttgacggttt gcgaaaatcc gctttttttt gcttggagcg
     1501 gattttccgc aataaattta gcaaattatg cacggaagta ttttcgccaa agtttttttg
     1561 atccttatcg cggtggacct gctcctggac tgctatactt ttatggggat caggacgctg
     1621 acaaatacgt ggaagtctgc cggtttgcgg caggtggtca actggggctg gctgggggtc
     1681 gtcgtgggga tcacggtatt gctggtcgcg ggtttcgaca gcttccgtca tccaagcggt
     1741 atgatgcctt accgggaatg ggttttgagc ctttttctcg cgctgttcgt tacgaaactg
     1801 ttctttgcga tcatattatt gctcggtgat atcggccggc tgctttatgg gttggtaaaa
     1861 cggctgctac gcccggcgga tgcggtggcg gacggatccc gttctatggc catcgggaca
     1921 gctaccggcg aacccctgat tcccgcacgc cggaaattca taagcgaatt gggggtgctg
     1981 gtggcggcag tgcccttctc gtcgtttggg tacgcgatgc tgcgcggcaa gtacgatttc
     2041 cgactacata agatagccct tcatttcagg gacctccccg aggccttcga tgggtttacg
     2101 atcacgcagg tgtccgatat tcattcgggc agcttcgaca acgtgcgcgc cgtgcaaagg
     2161 ggtatcgacc tggcgaaggc gcagaacagc gatctgttcg tatttacggg cgatttggtc
     2221 aataacgtcg cctgggagat cgagccttat atcgaccgtt ttgcggccct aaaggcgccg
     2281 tacggtcaat attccattct cggaaaccat gactacggag actatatcaa atgggacagt
     2341 ctcgacgata aggcgcgcaa tctggaagcg ttgaaaaggc atcaccgcga cctgggctac
     2401 cggttactga tgaacgaaca tgtgttgctg gaaaaagacg ggcagcgcat cgcgctcatc
     2461 ggcgtgcaaa actggggacg cgggttcatc cagctgggtg atctcgacaa ggcgctggaa
     2521 ggactgggca acgacgtctt caaaatatgt ctgtcgcatg accctactca ctgggaggaa
     2581 aagatccgtt ttcatcccac gacgatccat ctcaccctgt ccggccatac ccatggcgct
     2641 caattcggcg ttgagtccga ccagtggaga tggagcccgg tgcaataccg ctatctcgat
     2701 tgggccggac tggccaatga gcgtgaccgt tatctgtacg tcaaccgggg attcggcttt
     2761 cttgcctttt caggtcgtct gggcatctgg cccgaagtta cggtcatcac attgaaacga
     2821 ggggtctgat cctccgctcg aacaggctta tgccgctgcc caggtaatgt cgcggcacgg
     2881 cgggatcggc gtcatcgggg aattgtttga cgccgcaata ttcgaaaccg cactcgcggt
     2941 aataggcatt gagccgctgg ttgtcgcggc cggtatccag ccggatatag tctttcccca
     3001 tcgaccgccc ataatcttct gcccatgcca cgatgtgtct gacatagccc gcgccccgga
     3061 aaaggggatt ggtgacgatg cggtgcagat agatcgaagg atcgacgtca cgctcgtccc
     3121 agaccacgcg gtccgtaaag gcgatggaaa agaaacacgc gatcacgccg tccccgatga
     3181 tcttccagtg caagcgttcg gcgatctccc tttcgatcag cgggcgatcc attccctgcc
     3241 aggaatagga gcccttgctt cgctggtatt cgatggcgag agtgaacaat gtttcaatgt
     3301 cggcgagatc gccgggctcc gtcggagtga tcgtcatggc tacaagatag cgatttcgtc
     3361 ggcacgctcc cgtgtcctac agggccgcaa gcgcctggct ggcggcattg atctcctcgt
     3421 aatgctggaa ataaatagcc gtagcgataa agtgcttgac atgtgcccgc gattccttcg
     3481 ggagataacg ctccagcgcc cagaaattat ggctgccgct gcgatgaatg gccctgatca
     3541 ccgggagcgg acctgcatta tatgccgcca gtaccagcag ccagtctttg aactggctgt
     3601 gcagatcgcg gagatacaag gccgccgccc tggtgctttt gtaaagattg gtccgttcgt
     3661 cgtaacggcc atttaccttc agacccagat ctctggcggt gccggccatc aattgccagg
     3721 ctcctcttgc gccgacgcgg cttactgcgc tggtcttcaa attcgactcg atgacggcca
     3781 ggtatttcag gtctgtaggc agaccgtaat ggacgagtac cgagtcgatg atggtaaacg
     3841 atcttttgct gtgttgtttg accagcgcga gatcttcgtc gttgtcgtgg atataggcat
     3901 ccacgaactt gagcgaacgg ttttggggca gggcaatagc gccaacggat tttgcagccg
     3961 tgagcgaaac ataagcagtt gcaggaggcg ctgcgttcgc ctgctgcgat gcgggttggc
     4021 cagtgttggc cgctgtggga agtccgatga ccagcgttaa ataaacgggt accttcaaca
     4081 tcgttttcat tttagggtaa aacaatgccg gtcccaggaa tggatcaata ctgcgtgaga
     4141 gggttaaaca tagactagtg gggaaagcat gccagccgtg ggtacggtga atgggttttt
     4201 cattcacggt ggctatacgt gacgggtaaa gataataccc gccggtggta tctcataagt
     4261 agttttacta gggtttttac tatattcggt acatgagaaa gccgctttta ttcctcgcct
     4321 gtctgttcaa tatgctgatt accaatgcgg cacccgcgcc cagggactac ctggtccaca
     4381 gtcccgacgg gtcgcttacg gtgcgtatat ccgtggatga acatacccga tggtcagccg
     4441 aaatggacgg acagcccgtt atcaccccgt cggctatctc gctgcggctc tccgatggcg
     4501 ttatcctggg ggataatatg aaggttgtat ccgcccccgt tcaaaccgtc gatgtcaact
     4561 tcgatcctgt caattacaaa aaatcccata tccgggacca ctataaccag ttgacactga
     4621 atgtaaaagg cgactatagt gtcgttttca gggtgtacga cgacgccgtg gcctatcgct
     4681 tcgtcacccg gaaacaaggt gagctcatcg ttcgtgacga aggagccgaa ttcaattttg
     4741 cagccgatga cgatgccttc atccctatcc aatgggatta tagggcgggc aagaatttca
     4801 attcctcttt cgaagcgttg tatcacgaga tccatctgtc gcagatgccg aaggactcgc
     4861 tggcattcct gccgctgctg gtcgatctgg ggcaaaacaa gaaggcggtg atcctcgagg
     4921 ccgatctgca ggactatccg ggcatgtatc tcggcgccaa cggaaatgga ctgaagggcg
     4981 tctatgctcc ctatccactg gatgccgcgt tgcacagcat caattatatt cccggcacac
     5041 gggcggatta tatcgccagg accacgggta cgcgcaattt cccctggcgt gtcgtcgtcg
     5101 tgagccgcca ggacaaggat ctcctcaacc aggatatcgt ccagaaactc gccgaaccgt
     5161 cgcaactgaa ggatatcgga tgggtacggc ccggacaggt ggcgtgggac tggtggaaca
     5221 actggaatgt ttcgcatgtc gatttcaaag cgggcatcaa taccccaact tataaatact
     5281 atatcgactt cgccgccgcg aaccatatcc cctacatcat catggacgaa ggctggtcga
     5341 acgatctcga tctgatgcaa cagtcgtcgc ggatcgattt gaaggagatc atcgactatg
     5401 gcaaacagca cggggtaggg gtcatcctct gggccacgtg gtatgccgtc acccaccaga
     5461 tggaaaaggc ctttcctttt tacgccaaca tgggcgtgaa gggatttaag atagatttta
     5521 tcgatcgcga tgaccaggtg gctatcgcat cgacctatgc catcgcgcgt ctcgccgcgg
     5581 ccaacaacct gatgatcgac tatcacggca catcgaaacc tgcgggtctg caacgtacct
     5641 ggcccaatgt catcggctat gaaggggtaa aaggcctgga gaactttaaa tgggcgaatg
     5701 aggaccagcc gcgatatgtc gcaaccatcc cgtatatccg aatgatggcc ggaccgatgg
     5761 attatacgcc cggcgcaatg cgcaatgcca cccaatccgc gtaccgtccc atcaacgaca
     5821 atccgatgag caaggggacg cgttgtcagc agctggcgga atacatcgtc ttcgaggcgc
     5881 cgttacagat gttgtcggat aacccgacca cctacaggaa agaacaggaa tgcaccgatt
     5941 tcatcaccaa agtgccaact accttcgatg agacgacggc gctggatggt aaggtcggcg
     6001 aatatgtggc gatcgcgcgt cgcaggggcg acacgtggtt cctcggtgcg atcaccgatt
     6061 ggacaccgcg gcagctgacg atcgattttt catttcttcc cgccgggcat tatgaagcgg
     6121 aggtgttcca ggacggcgtt aacgcggacc gtgatgccac cgattaccgg cgacaggtga
     6181 tatccgtgaa atccggagat aagctgtctg tgcagttgta tggaggcggt ggatgggtcg
     6241 caaggatcta cccggtgaag taacgtcagt tcggctgtac cagcagttca ttggatgcct
     6301 gcagcgaagc cgccgactcg ctcatcagcc tggtgaattt atcattgtcc ttctcctgca
     6361 aaatggcgct gggcgttggg gtcgtcgcgg cagcgttcgc gctatgttcg aaataataag
     6421 cagtagcgat gaatttcttc acatgctgtc tcgactccgc gggcagatag cgttgcagga
     6481 cccaaaaatt ccggctatgc gattttcgta tagcccggca gaccggtctg ggaccgccgt
     6541 tgtatgccgc aagcacgagg agccagtccc cgaattcacg atgcaggtca cggagatatt
     6601 ttgcagcggc ccgcgtactc ttgtaatagt ccttccgctc gtcggaacgg cggttgactt
     6661 tcagacccaa aacatgagcc gtggatcgca tcagctgcca gggacctttc gcaccgacac
     6721 ggctggtggc agtgggtttc agctccgact cgatgacggc aagatatttc agctgttggg
     6781 gcaatccata atggtccagc accgagtcga tgatcgcgaa cgggccatcg cttcgttgtc
     6841 tgacgcttgt aagacaatcg tcgttattcc ggatgtagct ggaaacgaat ttcatcccct
     6901 tttcgggtat cggcacggag atcagctgtt tttttgcaac tgccgttctg tgcttgccac
     6961 cggcatgggc gacatctttg gccgtatcgc ggtagggttg accgggattg gcatccgagg
     7021 gcacgcctac gaagaaggct atgctacaaa cataaaaggg tagtggcgac ttcaacatcg
     7081 ttttagtttt tgggtaaaac aatgccggcc ccaggtaatg gattattcgg aatttaacat
     7141 agcatattgg aagatggaac tatagtcagg cctttcgtgg gtacggtaat tagggttttt
     7201 cattttcgta aggcggtgac cggggcaaag atagttcccg gccgtccacc gccatagggg
     7261 gttttaccag ggaaattacg taggaaaagc ctcatggaaa ctcgtatatt tacgaaaaca
     7321 ccatgacacc atccatcgga aaccccgtcg ttcatttcga gatcggttgt aaagacctcg
     7381 aaggcaccac ctccttttat acaaaacttt ttggttggac ggcctccacc gtccctactg
     7441 ccgccatatt ggagactcat tcaacgaatg gcatccccgg tcacattaca tccctgggtc
     7501 atgaaccaca tcaatacgtc accttttata tcgaggtgga cgatatcgat gaaacgcttg
     7561 gacggatagc gggcgcggga gggaaaaagg tcatcggacc ttttccattg cccgatggga
     7621 agaaattcgc ctggttctcg gatccgggag ggaatatgat cggcctcatc acgaagacct
     7681 agctcgcccg tcccgtaact ttcgtcggga accggaaggt atagatgccg atgacaagga
     7741 tgacccagca gcaaagcggt aattgcagcc attgttcgag cgaactaccg ggattgtgcc
     7801 ctgcccagtc gtacagcagc cagaaaggga cgaaggtcag cagggccgtc catgcgcaaa
     7861 gcaatagccg ctgggtaggc acggacagtt ggctggttcg cagacaggcg gcgccgcccc
     7921 agaaaaaaag agcgctcggc agagataaga taaagcctga caggatcgcc gcgatggtaa
     7981 agccatagaa attcagcagc ccttcgaggt tggaccatga ccaggcgaaa tacagcatgg
     8041 ggcctagcac caggctcgtc agccagactt tcgcacaata gaaaaggacc ttttttgaca
     8101 tggcgggggg ttctgatacc aatttaccaa atatttgaga atgacggtgc ggaatggtgg
     8161 cacacggggg caaaatcatg gaatgacggg ctgctaattg ctttagccct ttttaatccc
     8221 cgaaaaccgc aagaattcct atcttaccat cctgacaaag tgcaatgatc atggcaacga
     8281 gtgaacatat ccttaccatc gatgtaggag ggtctcatat caagggcacc atcctcgaca
     8341 gcgagggaaa ccaacagatg aagtacaaga aattggtgac gccggcgaat gcgacgccgc
     8401 acgcggtgct gaccaccatc ctgtcgctgg tggaaggcat gccggaatat ggcagggttt
     8461 cggtcgggtt tcccggatat gtgcggaacg ggatcgtata cacggcgccc aatctgcaga
     8521 acctcttgtg gaaacatatc gaattcggcc aacagataag cgacgcgttg cagaaaccgg
     8581 tgcggttggt caacgatgcc gatcaattgg ggcttggggt cgtcagtggc gaaggttttg
     8641 aactggcggt cacgctcggc acgggtttcg gaaccgcagt cctcatcgat gggtatctgc
     8701 tgccgcatct cgagctggcg catcatccac ttcataaagg aaaggattac gacgcctata
     8761 tcggcgaaaa ggccatgaaa aaggcgggga ggaagaaatg gaaccaacgc gtgaagattg
     8821 tgttggagat actgaagacc gtattcaact atgatcggct gtatctcgga ggaggtaatg
     8881 cctcgaagct gacgatcccg ctggacggga atatccatct ttttaccaac gaggacggga
     8941 tacggggagg agccaggttg tggaaaatgg aagataaata tcatatcact acgaattacc
     9001 cgaaacccgg aggaacggca tgaataaacc agaatcggaa aggctgaagg agcccgggtt
     9061 aaaactgtcg acattttttg agatgactcc cgatcttgtc tgtgtggcag ggcgggacgg
     9121 atattttaaa aaggtgaatc gtgccgtcct ggaagcgttc ggctatacgg aacaggagct
     9181 gatgagcagg cccatctcct cttttatgca cccggaagac agggagctca ctttgcacaa
     9241 acgcaacgag ctattcgacg gcaaagcgct ggtcaatttc cagaaccgat atgttacgaa
     9301 gacgggggcg atcatctggt tgcactggac atccatctac ctggaggaca gcggagtggt
     9361 cttcgccatc gccaaggata tcaccgaaca gaaaaagatc gagctcgaca tcgaggcgaa
     9421 atataaaaag ttcaagggtc ttgccgcgca tttcaagacc agcatggaag aggaccggaa
     9481 atatcttgcc cgtgagctgc atgaagagct ggcgcagctg gcttccgtcc tgaaaatgga
     9541 tatcgaatgg gtgaagaacc attcgcccga gctctcgcca gcatcgaaag acaggataga
     9601 gcatgccgcc gtaatttctc agatgctcat cacgacgata cgacggcttt ctttttacat
     9661 cagcccgaac atgctggagc atatcggtct caccgcaacg atggaatggt attgtcgcga
     9721 attctcgatc ctgaccggta ttccctgccg gttcgaaagt gaattcaacg aaaaaggatt
     9781 gacggaagag atcaggatcg atctgttcag gatctgccag gaggctttga tgaatacgct
     9841 gaatcatgcg caagccaatc atgttacggt acggatcgaa gaccgggtac gacagctgca
     9901 attgactgtg acggatgatg gccagggatt cgatgtcgct aaacagcaac ggaaatcagg
     9961 aatgactcgc atgcgcgagc gtgcggcctc gatcaatgcc cagctgacta tcgacagcgc
    10021 tccgggcaag ggtaccacgg tagcggtgac catatccaag tgagctcagc actaaatgct
    10081 aaaatcgctc ggatttatgc gttttctttt atatatttga aacgctaaac cctctgatgc
    10141 gcccatccat ttccatgaaa gccctggcag gagtcctgtt cctgtttgtt ttttgccatt
    10201 ctcccgtggc gggccaaatg cgtcagatct acctggaccc ggattcaacc aaccatatca
    10261 acaggctgag cttttattcc cccaatgaag gatacgtagc attcgagcat tggatcggct
    10321 atactacgga cagcggcagg tcatatacga agatctatat caccccaaac aatgtcaatt
    10381 tcaacggtta tagttcggcc aattgggtcg tcgggttctt tatcaacgga gtaaaagcca
    10441 tttccaaaaa tatcctgatc gtctacggga attacgactt agtgccatcc atcctgtatt
    10501 cttccgacg
//
LOCUS       DASYSV010000386         4115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_975700, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000386 DASYSV010000000
VERSION     DASYSV010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975700"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VGQ51_11880"
     CDS             <1..596
                     /locus_tag="VGQ51_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV9037319.1"
                     /translation="IARLAQLARAVGIHLIIATQRPSVNIITGTIKANFPARIGFKVS
                     SKIDSRTILDAGGADQLIGKGDMLISYNGEITRLQCAFVDTPEVEAITQFIGRQRGYP
                     DAYKLPEYVDEKEMEAKGFDLADRDPLFEEAARLIVLNQLGSTSLLQRKMKLGYNRAG
                     RLMDQLEAAGVVGPGMGSKPRDVLIKTEMELDQYLNP"
     gene            621..1304
                     /locus_tag="VGQ51_11885"
     CDS             621..1304
                     /locus_tag="VGQ51_11885"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like putative outer membrane lipoprotein
                     chaperone"
                     /protein_id="HEV9037320.1"
                     /translation="MNTVNTKSALLSLTILCFGIFAQAQNNSLGKNDPGAKKVLDGLS
                     ARLKSYKAVKSGFTLKVQDQKGAVMGSKSGTIYLKGSKYHINIPGGQDIYCDGKDTWT
                     YDKSSNEVTITKSDPSTQTLSPDKFFTNFYDKDFLYKLNGESKVGGKTVQEVELTPTD
                     KTKSFFKAFLYIDKTTHTLVSVKWLDKGGNSYTLTINRLNGNAPLTDADLAYNKAKHP
                     GAEEVDLRN"
     gene            complement(1301..1858)
                     /locus_tag="VGQ51_11890"
     CDS             complement(1301..1858)
                     /locus_tag="VGQ51_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEV9037321.1"
                     /translation="MRKIVVAITGASGSIYARLLLEKLALLHSQWETVAVVMTDNAKQ
                     VWRTELDTSRYEDCGFPFFSQQDFTAPFASGSGKFDTMIIVPCSMGTLGRIATGMSND
                     LITRAADVVLKERRKLICVIRDTPYNLIHIRNMETVTLAGGVICPATPSFYSRPRTIE
                     EAAATVVDRVLDLSGLNIDTFRWGA"
     gene            1888..2415
                     /locus_tag="VGQ51_11895"
     CDS             1888..2415
                     /locus_tag="VGQ51_11895"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEV9037322.1"
                     /translation="MLRGKSIFLFAALVALLSAWTVPTGRESISWMTIEQAETQLHQQ
                     QKPILIDLYTSWCGWCRQMDRKTYSNKKVAQYLSDKFYTVKIDAETKATISWQGKTYT
                     FDPQYRCNEFAVYLSHGQLEFPTTVIIAPGEAPQAIPGYLEPKDMEMLVKYYGEGVYK
                     TRGFDEYAKGFHATW"
     gene            complement(2686..3942)
                     /locus_tag="VGQ51_11900"
     CDS             complement(2686..3942)
                     /locus_tag="VGQ51_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV9037323.1"
                     /translation="MPDIFEKLVKHNGPIGQHRERAHGYFAFPKLEGEIGSRMTFRGK
                     EKIVWSLNNYLGLANHPEIRKVDAEAAARFGMATPMGARMMSGNTSYHEQLEKELAAF
                     EGKEDATLFNYGYQGIMSAIDAVCGRHDVIVYDAESHACIIDGLRLHPGHRYVFKHND
                     IEDCEKQLQRATALIEKQNAGGILVISEGVFGMAGDQGKLKEIVALKDTYEFRLLVDD
                     AHGFGTLGKTGAGAGEEQGIQDQIDLYFGTFAKSMASIGGFMAGDKAIIDYIRYNTRS
                     QIFAKSLPLPVTIGNLKRLELLRTKPELRKKLWDVVNKLQKGLKDSGFDIGKTNSPVT
                     PVYMKGDVPEATAMVMDLRENHHIFCSIVVYPVIPKGHIIYRLVPTAVHTDEDIERTL
                     AAFRATKKKLDAGQYKVQTIPDMAEA"
BASE COUNT         1000 a         1041 c         1067 g         1007 t
ORIGIN      
        1 cgatcgcacg tctcgcacag ctggcacggg ccgttgggat acatctcatt atcgccacac
       61 agcgcccgtc cgtcaatatc attacgggga ccatcaaggc caatttcccc gcccgcatcg
      121 gcttcaaggt gtcctccaag atcgatagtc gtacgatcct cgacgccggc ggcgccgatc
      181 agctgatcgg aaagggagat atgctgatct cctacaatgg cgagatcacg cggctgcagt
      241 gtgcctttgt agacaccccc gaagtggagg ccattacaca attcatcggt cgtcagcggg
      301 gttatccgga tgcctataaa ttgccggagt atgtagacga gaaagaaatg gaagcgaagg
      361 gattcgatct ggccgaccgc gatccgcttt tcgaggaagc ggcccgtctc atcgttctca
      421 atcagctggg atcgacctcg cttctccagc ggaaaatgaa gctgggctat aaccgggccg
      481 ggaggctgat ggatcagctg gaagccgccg gtgtggtcgg tcccgggatg ggcagcaaac
      541 cgagggatgt gctaataaaa accgaaatgg agcttgacca gtacttgaat ccatagggat
      601 atgcgtatct ttgcgcgcaa atgaacactg tgaacaccaa atcggcccta ctcagtctaa
      661 cgatcctttg cttcggcatt ttcgcgcagg cgcaaaacaa cagtctcggc aaaaacgatc
      721 cgggtgcaaa aaaggtgctc gacggtctca gcgccaggct gaaatcgtac aaggcggtaa
      781 agtccggctt taccttgaaa gtacaggatc agaagggcgc ggtcatgggt agcaaaagcg
      841 gcactattta cctgaaaggc agcaaatatc atatcaatat tccgggtggt caggacatct
      901 actgcgacgg gaaggatacc tggacctatg ataaatcatc caatgaagta acgatcacaa
      961 aatcggaccc ttccacgcag acccttagtc ccgataaatt cttcaccaat ttctacgata
     1021 aggatttttt gtacaagctc aacggtgaga gcaaggtcgg cggaaagacc gttcaggaag
     1081 tggagctgac acctaccgac aagacgaaat cgttctttaa agcctttctt tatatcgaca
     1141 agaccacgca tacgcttgtc agcgtcaaat ggcttgacaa aggcggtaac agctatacgc
     1201 tgactatcaa taggctcaat gggaatgcgc cgctgaccga tgccgatctg gcctataata
     1261 aggcgaaaca tccgggcgcg gaagaggtgg atctcaggaa ttaagccccc cagcggaatg
     1321 tatcgatgtt caaaccggac agatcgagga cgcggtctac caccgtggcg gcagcttctt
     1381 cgatggtcct gggtctgcta taaaaagatg gcgtagcagg acagatgacg cctcccgcca
     1441 gcgttaccgt ctccatgttc cggatatgta tgaggttgta gggcgtatcc cggataacac
     1501 aaatgagctt acgtctctct ttcaaaacta catccgcggc gcgggtgatc agatcgttgg
     1561 acataccggt tgcgatgcgt cccagtgttc ccatggagca ggggacaatg atcattgtat
     1621 cgaatttccc ggagccggag gcaaagggcg cagtaaagtc ctgctgtgaa aagaagggga
     1681 atccgcagtc ctcgtaacgg gaggtatcca attccgtccg ccatacctgt ttggcgttgt
     1741 cagtcatgac tactgcgaca gtctcccatt gggaatgaag aagagccaat ttttccagaa
     1801 gcagccgggc atagatgctg ccgctggcgc cggtgatggc cacgacaatt ttgcgcatgt
     1861 gttactcgtt atcctacaaa agtatatatg ctaaggggca aatccatttt tttattcgcg
     1921 gcgctggtag ccttgctgtc ggcctggacg gtccccaccg gcagggaatc catctcctgg
     1981 atgactatcg agcaagccga gactcaactg catcagcagc aaaagccgat cctgatcgat
     2041 ctttatacgt cctggtgcgg atggtgcaga caaatggacc ggaagaccta ttccaataag
     2101 aaggtggccc aatatctctc cgataagttt tatacagtaa agatcgacgc ggaaaccaaa
     2161 gccactatta gctggcaggg caagacctac accttcgatc cgcaataccg ctgtaatgaa
     2221 tttgcggtct atctttcaca tggtcagctg gaattcccga ccaccgtaat tattgcgccc
     2281 ggtgaagcgc cacaagccat tccggggtat ttggagccaa aggacatgga gatgctggtg
     2341 aaatactacg gggaaggcgt gtataagacg cgggggttcg atgagtatgc taagggtttt
     2401 catgcaacct ggtagggcag agggttagcc ggaaaaggtt cgcgaatagc tacaggggtc
     2461 tatgtgtaaa gtttacactt gatctggatc aatttaatgg tttccaatcg aaaatactgt
     2521 ttcatggatt cgtatgatac gcgattgtga tattatcttt ggtctttcat aggataaggt
     2581 ttaatggtta atggtatttg attagtgcag ccccgcgtaa gcggggcttt ttcgtataag
     2641 caaaaacccc gaccggaggt cggggtttca agtcttttgt acttcttagg cttcagccat
     2701 atcggggatg gtctgcacct tgtactgccc cgcatccaat ttctttttgg tagccctgaa
     2761 tgcagccagg gtcctttcga tatcctcatc ggtgtgtacg gcggtgggca ccaggcgata
     2821 gatgatatgt ccttttggaa taacgggata gacgacgatg gagcagaaga tatgatggtt
     2881 ttcgcgaagg tccatcacca tggccgttgc ttcaggcacg tctcctttca tgtataccgg
     2941 ggtgacgggg gaattggtct tgccgatatc gaaaccgctg tctttcagtc ctttttgcag
     3001 tttgttgacg acgtcccaca actttttgcg caactcgggt ttggtgcgca ggagctcgag
     3061 ccttttcaaa tttccgatgg tcaccggcag cgggaggctc ttggcgaaga tctgcgaccg
     3121 ggtattatac cggatatagt cgatgatggc cttgtctccc gccatgaaac ctcctataga
     3181 agccatcgat tttgcaaaag tgccgaaata aagatcgatc tggtcctgga taccctgctc
     3241 ttcacctgca ccggcgccgg tcttgcctag cgtcccgaac ccatgcgcgt catccacgag
     3301 aagccggaat tcataggtat ccttgagggc aacgatctct ttcagtttcc cctggtcgcc
     3361 cgccattccg aaaacgcctt cggagattac caggataccg ccggcatttt gcttttcgat
     3421 cagcgcggtg gcccgttgaa gttgtttttc acaatcttct atgtcgttat gtttgaacac
     3481 gtagcggtgg ccggggtgga gccgcaatcc gtcgatgatg caggcatggc tttccgcgtc
     3541 gtacacgatc acgtcatgcc gtccgcaaac ggcatcgatg gcactcataa tgccctggta
     3601 accgtaattg aacaaagtgg catcttcttt tccttcgaaa gccgccagct ctttttccag
     3661 ctgctcgtga tagcttgtat ttccgctcat catccttgcg cccatggggg tcgccattcc
     3721 aaaacgggcc gcggcttccg cgtcgacctt gcgtatttcg ggatggttgg caagaccaag
     3781 ataattgtta aggctccaaa ctattttttc tttaccacga aacgtcatcc ggctgccgat
     3841 ctcgccttcc agcttaggaa aagcaaaata gccatgcgca cgctctctgt gctgtccgat
     3901 cggtccgttg tgtttgacta atttttcaaa tatatcaggc atattaagga attatcaatt
     3961 ttgacgtaag cgcaaaaata acgcatttga cgatgcctca attcaaatac ttttgcccct
     4021 gttttgaaac gcgatcacgc tctcatttaa cccccatttt atgaaattcg gatccttcgc
     4081 acgggacctg gaatccctac gatacgcata tcccc
//
LOCUS       DASYSV010000387         4729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_76943, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000387 DASYSV010000000
VERSION     DASYSV010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76943"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VGQ51_11905"
     CDS             <1..422
                     /locus_tag="VGQ51_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV9037324.1"
                     /translation="VLCGGEAFHQNYGLEHGYYIRPTLFGGYNKMRIFKEGIFGPVSC
                     VTTFGSTEEAIAIANDTAHGFGAGIWTRDPRVLYTVPRSIRADRVWVNCYPSYRGPFG
                     GLERPGFGGEVRKEVLARYRQTKNVLIAFSSERLGFF"
     gene            complement(448..879)
                     /locus_tag="VGQ51_11910"
     CDS             complement(448..879)
                     /locus_tag="VGQ51_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037325.1"
                     /translation="METYNLDSDVRLCCFTAESFPDGVMAAFQRLNSSVPDHEGRRLF
                     GLSWPDGKGSMVYKAAVEERYPGESEDLGAEPYILKRGEYLGLEVHNFMDDIPSIGRA
                     FRQLVDSPGVHPNTMGVEEYLSGTTVRCMVPMSLQNAWSNN"
     gene            1012..1989
                     /locus_tag="VGQ51_11915"
     CDS             1012..1989
                     /locus_tag="VGQ51_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV9037326.1"
                     /translation="MKRITILVPETAVVEAVADPRYIFTAVNEFLISAGKEPVFSVQL
                     VGLTREVRVTDGLFSVHVDAVLKDAQKPDLIIIPAISGNLDRALRLNQEFIPWITQQY
                     KDGSEVASLCIGAFLLGATGLLNGKSCSTHWLFANEFRDRYPEVELTDDKIVTEQSGL
                     YTSGGANSYWNLLLYLVEKFTSRELAILCSKFFVLDIGRASQSPFSIFKGQKTHNDAE
                     IVRVQEYIETNFQDKITVDELCDKFGIARRTFERRFKKATNNTVLEYWQRVKIEAAKR
                     QLEVSRKTVGEVMYEVGYNDTKAFRDIFKKVTSMSPVDYRNKYNKEAMN"
     gene            complement(2075..2632)
                     /locus_tag="VGQ51_11920"
     CDS             complement(2075..2632)
                     /locus_tag="VGQ51_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037327.1"
                     /translation="MTTLKKLILSASACMIAATLHAQIEATQLIMKGQSATGLGGFFH
                     GGFPVAKGDEISLEAGFQYFAPEQSHLIFIPLLAGYRYFFDHSGTRWYIEPFAGYTLG
                     NTDIQKLDASGNPVFTSDSSDVDQKASGPIAGLGIGYILPSATLPLNFGLRFEHVFVS
                     GDPAASLLSLRISWSIFTARRLATQ"
     gene            2681..3316
                     /locus_tag="VGQ51_11925"
     CDS             2681..3316
                     /locus_tag="VGQ51_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEV9037328.1"
                     /translation="MKKAFLLCGLGLCLLIGESSRPARSPWISLFDGKTLDGWKVGEN
                     ASTFTVDSGCIIAHGQTAHLFYEGDVHHHDFKDFDFKAEVMTRPGSNSGIYIHTAYQE
                     GGWPAKGYEVQVNNSHTDWRRTGSLYGIVDIREVYCPDNVWFTEAVSVRGKHIVIQLN
                     GKTVVDYTEPDNVQRPKGSEERVLSHGTFALQGHDPNSKVYFKDIMVRPVD"
     gene            complement(3313..4554)
                     /locus_tag="VGQ51_11930"
     CDS             complement(3313..4554)
                     /locus_tag="VGQ51_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV9037329.1"
                     /translation="MIKKDAIVTIRYSMWNGRGDLLENNQATYTHGSSAISARLQSQL
                     EGLQAGDSRVIHLKKGEEGADDDFTFEVFIDDVRETRAPLSIHLLSGFLGSGKTTAIA
                     QACRFLAKKGVATGVITNDQGSRLVDGDLFIHLGIPSGQVTNGCFCCNYDDLEKAICF
                     LSSHHGPSVLFAESVGSCTDLVATVLKPLLRRHPEWRPTISVFVDPQHTGTAFDPAIS
                     YIYNKQLEEAPLIIVTKTDLYPAARLAYADKTILYQNSFNDEDIGRWLQTLESQNPDL
                     DSIALDYDLYGAGEAKLAWLDQQVEIISRDSTAQHAAISLIRRVSQTPYPIGHLKFLL
                     DGHTKISITSTGATDHPDLRSAEKTTLLINARVQAEPAALSQLLTDAIRATETEDHCT
                     IHTITESCFQPGYPRPTHRIA"
BASE COUNT         1014 a         1024 c         1587 g         1104 t
ORIGIN      
        1 gcgttttgtg cgggggtgag gcctttcatc agaattacgg gctggaacat ggttactata
       61 tccggccgac gctgttcgga gggtataata aaatgcgaat ttttaaggag gggatcttcg
      121 ggccggtgag ttgcgtgacg acattcgggt cgacggagga agcgatcgca attgccaatg
      181 acaccgcgca tgggttcgga gcgggtatat ggacgaggga cccgcgggtc ctttataccg
      241 tccccagatc gatcagagcg gaccgagtgt gggtaaattg ttatccttca taccgggggc
      301 ccttcggagg actggaaaga ccggggttcg gcggtgaggt gcgaaaggag gtgctggcgc
      361 gttaccggca gacgaagaat gtgttgatag cgttcagtag tgagcgtttg gggttctttt
      421 aggggttggc ccggcgtggg gtgcgtgtca attgttcgac caggcatttt gcaatgacat
      481 cgggaccatg cagcggacgg tcgtaccgct gagatattcc tcaacgccca ttgtattcgg
      541 atgtacgccc ggcgagtcga cgagttgacg gaaggctctg ccgatgctcg ggatgtcgtc
      601 catgaaattg tgcacttcca gaccgaggta ttcgcctctt ttcaggatgt agggttccgc
      661 ccccagatcc tccgattctc ccgggtaccg ctcttccacc gcagccttat agaccatgct
      721 acccttaccg tcgggccagg agagtccgaa cagccgtctg ccttcatgat cggggacact
      781 ggaattgagc cgttggaagg ccgccattac cccgtcgggg aacgattcgg cggtgaagca
      841 gcacaggcgg acatcgctgt caaggttgta tgtttccatt tgaaatgcgt ttgcttaaca
      901 agacaaagct aggctggcgg accggaatgg atggcgtgta aatccgtcaa tcaaaggggt
      961 tgtttgcgac aggatttgac gttatcttta tagggcctaa atacgagcac catgaaacga
     1021 attaccatcc ttgtcccgga gacagcggtt gtggaggccg tcgccgatcc ccgttatatt
     1081 tttaccgccg tcaatgaatt cctgatcagc gccgggaaag agccggtgtt cagcgtccag
     1141 ttggttgggt tgacgcgcga ggtgcgggtg accgacggtc ttttttcggt gcatgtcgac
     1201 gcggtgctga aggacgcgca aaaacccgat ctcatcatca tcccggccat cagcggaaac
     1261 ctggatcgcg cgctgcggtt aaaccaggaa ttcattccct ggatcacgca gcaatataag
     1321 gacgggtccg aagtggccag cctgtgtatc ggcgctttcc tattgggcgc tacgggattg
     1381 ctcaatggta aatcgtgttc tacgcactgg cttttcgcca atgaattcag ggaccgatat
     1441 cccgaagtgg agctgacgga cgacaagatc gtgactgagc agagtggatt gtataccagc
     1501 ggcggcgcca attcttactg gaacctgctt ttatatcttg tagaaaaatt taccagccgt
     1561 gagctggcaa tattgtgctc gaaattcttc gttctcgaca tagggcgggc cagtcaatcc
     1621 cctttcagca ttttcaaagg gcaaaagacc cataacgatg cggagatcgt cagggtgcag
     1681 gagtatatcg aaactaattt ccaggataag ataacggtgg atgagttgtg tgataagttt
     1741 gggatcgcgc ggaggacgtt tgaaagaaga tttaagaagg ctacgaataa tacggtgctg
     1801 gagtattggc agcgggtgaa gatcgaggcg gccaagcggc agctggaggt gagtcggaag
     1861 acggtggggg aggtgatgta tgaggttgga tataacgata cgaaggcatt cagggatatt
     1921 tttaagaaag tgacgtccat gtcaccggtg gattaccgga ataagtataa taaggaggcg
     1981 atgaattaag gcggcggggt accagcggcg ggtgcggatg gggggcggat cgcgggggcg
     2041 gcgcatgcgg ggcggcagac ggcggatcgc ggggttactg tgtcgccaac cggcgggcgg
     2101 taaagatcga ccaggagatg cgcagggaga ggaggctggc tgcaggatcg ccggagacga
     2161 agacgtgttc gaagcggaga ccgaagttca gcgggagcgt ggcggacgga aggatatagc
     2221 cgatgccgag acccgcgatg gggccggatg ctttttggtc cacgtcgctg ctgtcgctgg
     2281 tgaagacggg gttcccggag gcgtcgagtt tttggatgtc ggtattgccg agcgtgtagc
     2341 cggcaaacgg ctcgatatac caccgggtgc cgctatgatc gaagaagtag cgatagcctg
     2401 ccagcagggg aatgaatatc agatgggatt gctccggcgc gaaatactgg aaaccggctt
     2461 cgagactgat ctcatcgcct tttgctacgg gaaagccgcc gtggaagaac cctccgagac
     2521 cggtggccga ctggcctttc atgatgagct gggtggcttc gatctgtgcg tgtagcgttg
     2581 ccgcaatcat gcaggcagac gcagaaagga ttagcttttt gagcgtggtc atggcaatag
     2641 acattggatt tctgtccgga agataataaa ttaaaattat atgaaaaagg cttttttgct
     2701 ttgcgggtta gggctttgtc tgctgatcgg ggaatcatcc cggccggcca ggagtccatg
     2761 gatatcctta ttcgacggta aaacgctcga tggctggaag gtcggcgaaa atgcgtcgac
     2821 ctttacggtt gacagcggtt gtatcattgc gcatggtcag acggcgcatc tgttctatga
     2881 gggcgacgta catcaccatg atttcaagga tttcgatttc aaggccgaag tgatgacccg
     2941 gcccggtagt aattcgggca tttatatcca tacggcgtac caggaaggcg gatggccggc
     3001 gaagggttat gaggtacagg tgaataattc gcatacggat tggcgaagga cgggtagtct
     3061 gtatgggata gtcgatatca gggaagttta ttgtccggat aatgtctggt tcacggaggc
     3121 ggtgagtgta cggggtaagc atattgtcat acagctgaac gggaagacgg tggtggatta
     3181 taccgagccg gacaatgttc agcggccgaa ggggagtgag gagcgggtgt tgtcgcatgg
     3241 gacgttcgcg ttgcaggggc acgatccgaa tagtaaggta tatttcaaag atattatggt
     3301 aagaccggtg gattaggcga tgcgatgggt gggccgggga tagccgggtt ggaaacacga
     3361 ttcggtgatg gtgtggatcg tgcagtggtc ttcggtttct gtggcgcgaa tggcgtcggt
     3421 caggagttgg gatagggcgg ctggttcggc ttgaacacgc gcgttgatga gcagggtggt
     3481 tttttcggcg gatcggaggt ctgggtggtc agtggcgccc gtggacgtga tgctgatctt
     3541 agtgtgcccg tcgaggagga atttcaggtg gccgatggga taaggtgttt ggctcacgcg
     3601 gcggatgagg gaaatagcgg cgtgttgtgc ggtgctgtcg cggctgatga tctctacttg
     3661 ctggtcgagc caggccagtt tggcttcgcc ggcgccatag aggtcatagt caagggctat
     3721 agagtcgaga tcggggttct ggctttcgag ggtttggagc cagcggccga tgtcttcatc
     3781 gttgaacgag ttttgatata agatcgtttt atcagcgtag gcgagcctgg ccgccggata
     3841 gaggtcggtt ttggtgacta tgatgagggg tgcttcttcc aattgtttgt tgtatatata
     3901 gctgatggcc ggatcgaatg cggtcccggt gtgttgcggg tcgacgaaga cggagatggt
     3961 gggccgccat tcggggtgcc gccggagcaa gggttttaaa acggtggcga cgaggtcggt
     4021 gcaggatccg acggattccg cgaaaaggac gctggggcca tggtggctgg aaagaaagca
     4081 gatggctttt tcgagatcat cataattgca gcaaaagcag ccgttggtga cctggcctga
     4141 cgggatgccc agatgtataa acagatcgcc gtcgacgaga cgggacccct ggtcattggt
     4201 gattactcct gtggcgacgc cttttttagc gaggaaacgg caggcttgtg cgatcgccgt
     4261 ggttttcccg ctgcccagga agccactgag caggtggata ctgagcgggg ctcgcgtctc
     4321 gcgtacgtca tcgatgaaga cttcgaaagt aaaatcgtcg tcggcgcctt cttctccttt
     4381 tttgaggtga atgacccggc tgtcgccggc ctgcaagcct tccagttggg actgcaaacg
     4441 ggccgagatg gcagaggagc catgcgtgta agtggcctgg ttgttttcca ggaggtcgcc
     4501 gcggccgttc cacatgctgt accggatggt gacgatggcg tcttttttga tcatgctatt
     4561 ttggtctttt cgacagggat ggagccatag gctacatgta cggttgcgtg tccctgttgc
     4621 agtctgacgg tgccgaatcc ggtggcggta gaaaggaagc atgtaaaatc accgatgcgt
     4681 gagtcgatgt acagcgtttt gtcgacggcg tcatagcgca gaccggtga
//
LOCUS       DASYSV010000388         5767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_849282, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000388 DASYSV010000000
VERSION     DASYSV010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849282"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VGQ51_11935"
     CDS             <1..861
                     /locus_tag="VGQ51_11935"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV9037330.1"
                     /translation="AFEKIRQENILLAAGSTPLLGAAALAYFRPGCNVVSGDPTYADL
                     PSRAERMGAKWVKVPLTKDYALDLDAMEKAIDDDTVLVYICNPNNPTGTVVDTAKLKA
                     FCDRVSKRTVIFLDEAYMDYLPDPQGTTLIPMAVGGSNIIVARTFSKVYGFAGLRCGY
                     VVTQPDIIRRLQLYTEAFMSLSATTLQAAIAAYQEREYMEGVLKKTTASKEYLYSVLK
                     KEGYTYVPAVANFVIFPIKMDGKQFTDEMMKRSVGVRFWKFDNQDWCRISIGRMDEME
                     AFAAAFKEIS"
     gene            878..1858
                     /locus_tag="VGQ51_11940"
     CDS             878..1858
                     /locus_tag="VGQ51_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HEV9037331.1"
                     /translation="MKKQLIAACLLTAAVGVVRAQDTTAVRALSMQEYEKARTFSVAD
                     PDKDTYVKFENAYILDHSGFGKPYFITGDDGKRKRIDLYKLIRKEDRATIGTVIYYTT
                     EAGKRYTACMPDYRADTAVWRRYFEDVHAIEKQEPFFVLKLSYVLSKELGYQLYRGAR
                     TGQGPVDRSESGTYGNDICFPGDMRVTMADGGTKLLSDVKAGDAVVTVDPLTRRERVV
                     TVRELTVHPVKNYEITRLVLVSAVGQGDRDVVLSSRVLEATPNHPMGNGKTVGELKVG
                     DRVWCADGKAYREFMVWDKTEGAGGAQPVYNIIADGGSTIMLNGVMVRQK"
     gene            complement(1861..3207)
                     /locus_tag="VGQ51_11945"
     CDS             complement(1861..3207)
                     /locus_tag="VGQ51_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV9037332.1"
                     /translation="MGQTPLSPGRQTALGTDPSVPASGWPLPKQLTFRFFFLFFILYI
                     FFNPNGVVPFTEGLFGWYIQPFHQLIPWFGAHILHLSHPITVFTNGSGDTTYDFVILL
                     FIFVLSLLGCAVWSALHRHHHAYPTLYYWLSFILRYYIAVTMLSYGLFKVIKLQFPFP
                     GLSSLLEPYGKSSPMGLAWNFMGYSEGYNYFAGIAETLAGLLLLFRRTTIAGALLAFV
                     VCSNVMAMNYCFDIPVKLLSTMLVLMSLYLLSDNFSVIAGFLFKKRVTALKQPPAPPF
                     KKRSIRIGWTVGKSLFIAFVLIASVFQLIDARASYGDAAPHAAFRGIYDVRSFIRNKD
                     TLPPLVTDTFRWKQLVIDGSPTYPYASIRTINDSTKGYYSCKIDSVLHTMKFTSYRDS
                     TDQFIFAYHQPQNDSLILSGAWKKDSLEIRLKAYDWKNFLLVRRGFHWINEYPLNR"
     gene            3286..4320
                     /gene="proB"
                     /locus_tag="VGQ51_11950"
     CDS             3286..4320
                     /gene="proB"
                     /locus_tag="VGQ51_11950"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV9037333.1"
                     /translation="MQKPVLVIKLGTAVLTNEKGEIAGAIIRKVAAGIAALAGDYRII
                     LVSSGAVGSGRGFFSGYRGSLAERKAAAAVGNPILIQHYQRQFAAYGIPVAQALCERQ
                     HFSSRPRFLQLKETFAEFWKNGILPIMNENDLVSNVELKFSDNDELATLTAIGFDAEA
                     LILCTSVGGFMDGQGKILSEVEKVDSRIMGFVQTGKSALGLGGMLSKLTFTKLSTSLG
                     IRVIICGIGGKTPFSDALAGRTGTNFIPQASNLKARQKWLASGSITFGSLFVDRGAAR
                     ALMQRRSLLTVGVRKVQGKFGAGEVVQLVDEEENIVGVAKVRIGSAEITAGLSQKNVM
                     AAHADDIVVF"
     gene            4378..5622
                     /locus_tag="VGQ51_11955"
     CDS             4378..5622
                     /locus_tag="VGQ51_11955"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV9037334.1"
                     /translation="MKSIEPILLRVQKATPGLRSATDDQIAGVLRALADLLESNGALI
                     LRANTRDLKRQKPGDPRNDRLLLNEQRIRGIAGALRKVSGLPDPTGKELDRRTLANGL
                     EVERITVPFGVVGAIYESRPNVTFDIAALCLRSRNGCVLKGSQEAADTNKAAIGLIRK
                     ALSENGLSPDCVALLPADRDTVQQLMKAVRLVDLLIPRGSEGLIRHVRSNSLVPVIET
                     GAGVCHVYVEAEADLDMAAQIVVNAKVSRPGVCNAMDTLLVDREVAPVLLRRLEGQLG
                     YHEVEVYADREALRLLKGYRLLQRAKPEDYDREFLSLKCAIRVVGGMEEALEHIRVHS
                     TRHSEAIVSGNRDKCERFLREVDAAAVYANASTRFTDGEELGLGAEIGISTQKLHARG
                     PFALEKLVTEKWVIRGTGQVRG"
BASE COUNT         1361 a         1184 c         2008 g         1214 t
ORIGIN      
        1 gcattcgaaa agatcaggca ggagaatatt ttgctggcgg ccggatcgac gccattgctg
       61 ggggctgctg cgttggccta tttcagaccc ggctgcaatg tggtatcggg tgacccgacc
      121 tatgccgatc tgccttccag agcggagcgt atgggcgcca aatgggtgaa agtgccgttg
      181 acgaaggact atgcgctgga tctggatgcg atggaaaagg cgatcgacga cgataccgta
      241 ctggtgtata tatgcaatcc caacaatccg acgggaacgg tggtggacac cgcgaagcta
      301 aaggcgtttt gcgatcgggt gtcgaaaaga acggtgatat tcctcgatga agcctatatg
      361 gactatctgc cggacccgca gggaacgacg ctgataccga tggcagtggg cggcagcaat
      421 atcatcgtag cgcgcacctt ctcgaaagta tatggattcg cggggttacg ttgcgggtat
      481 gtggtgaccc agccggacat catcaggaga ctgcagctct atacggaagc ttttatgtca
      541 ctgtcggcga cgaccctgca ggccgccatt gccgcttacc aggagaggga gtacatggag
      601 ggggtgctca aaaagacgac ggcgagcaag gaatacctgt atagtgtttt gaaaaaagaa
      661 ggctatacct atgtgcctgc ggtggcgaat tttgtgatct tcccgatcaa gatggatgga
      721 aagcaattta ccgatgagat gatgaagcgg agcgtagggg tccggttctg gaaattcgac
      781 aaccaggact ggtgccggat cagcattggc cggatggatg aaatggaagc attcgcggca
      841 gcattcaagg aaatatctta aaaaatcgat caggtacatg aagaaacaac tgattgcggc
      901 ctgtctgctg acggcggccg ttggggtcgt gcgcgcccag gatacgacgg cggtccgcgc
      961 cctgagcatg caggaatacg agaaggccag gacattttcg gtggccgatc cggataagga
     1021 cacttatgtc aaattcgaga atgcgtatat cctggatcac agcggattcg gcaaacctta
     1081 ttttatcacg ggcgatgacg gcaagcggaa gcgtatcgat ctgtacaaat tgatccggaa
     1141 ggaggatcgg gcgacgatcg ggaccgttat ctattatact accgaggcag gcaagcgtta
     1201 tacggcctgc atgccggatt accgggcaga tactgctgtt tggcggaggt actttgaaga
     1261 tgtacatgcc atcgagaaac aggagccttt ttttgtgttg aagctgtctt atgtgttgtc
     1321 gaaggaattg ggttatcagc tgtatagggg cgcgcggacg gggcaggggc cggtggaccg
     1381 gagcgagagc gggacgtatg gaaatgatat ttgtttcccg ggggatatgc gggtgacgat
     1441 ggcggatgga gggacgaagc tgttgagtga cgtgaaagcg ggagatgccg tcgtgaccgt
     1501 ggatcctttg acgcgccggg agcgggtggt gacggtgcgt gagttgacgg ttcatcccgt
     1561 gaagaattat gagatcacgc ggctggtgtt ggtgtcggcg gtcggccagg gcgatcggga
     1621 tgtggtcttg tcgtcgaggg tgttggaagc gacacccaat catccgatgg ggaatgggaa
     1681 gacggtaggg gaattgaagg ttggggatag ggtgtggtgt gctgatggaa aggcgtatcg
     1741 ggagtttatg gtatgggata agaccgaggg cgccggaggg gcgcagccgg tgtataatat
     1801 catagcggat ggggggagta cgataatgtt gaatggggta atggtgaggc agaaataggc
     1861 ttaccggttg agcgggtatt cgttgatcca atgaaatcca cggcggacca gcaggaagtt
     1921 tttccagtca tatgctttca gcctgatctc gagggagtcc tttttccagg cgcccgacag
     1981 gatcaggctg tcgttttggg gctggtggta agcgaagatg aactggtcgg tgctatcgcg
     2041 ataggaggtg aatttcatgg tatgcaggac cgaatcgatc ttgcagctat aatagccttt
     2101 ggtgctgtcg ttgatggtcc ggatgctggc atatggatag gtgggtgagc cgtcgatgac
     2161 gagctgtttc caacggaagg tatcggtcac gagcggggga agggtgtctt tattgcggat
     2221 aaaggacctg acatcgtaga tcccgcggaa ggcagcatgc ggcgccgcgt ctccgtacga
     2281 ggccctggca tcgatcagtt gaaatacgct ggcgatgagc acgaaggcga tgaacaggct
     2341 ttttccgacc gtccagccga tgcgtatgct cctttttttg aagggagggg caggcggctg
     2401 tttgagggca gtgacacgtt tcttgaaaag gaagccggcg atgacggaaa aattatccga
     2461 aaggaggtaa agcgacatta ggacgagcat ggtggacagg agcttgacgg ggatgtcgaa
     2521 gcaatagttc atggccatga cattcgagca aacgacgaat gcgagcaagg cgccggcgat
     2581 ggtcgtccgg cggaaaagca ggagcaaccc ggccaacgtc tccgcgatgc cggcaaaata
     2641 gttataacct tccgaatagc ccatgaagtt ccaggccaga cccatgggag aggattttcc
     2701 gtagggttcg agaaggctgg agagaccggg gaaggggaac tgcagcttga tcactttaaa
     2761 gaggccatag gacaacatcg tgactgcgat gtaatagcgc aggatgaagc tgagccagta
     2821 atatagtgtc ggataggcat ggtgatgtcg gtgaagcgcg ctccagaccg cgcagccgag
     2881 gagagacagt acgaagatga agaggagaat gacgaagtcg taggtcgtgt ctcccgagcc
     2941 gttggtgaaa acagtgatcg gatgagagag gtgaagtatg tgtgcgccga accatggaat
     3001 gagctggtgg aaaggctgga tgtaccaacc gaagaggccc tcggtaaagg ggacgacgcc
     3061 gttggggtta aaaaagatat aaagaatgaa gaagaggaag aagaaccgga aggtgagttg
     3121 tttcggtaag ggccagccgc tggctgggac tgatggatcg gtgcccaggg ccgtttgcct
     3181 gcctggggaa agaggtgttt gtcccatgcg acgaggtttg ccataagata agcgattgaa
     3241 gtggaaattg caagagggtg tggggagaat ggggtagatt tgcggatgca aaaaccggta
     3301 ctggtcatta aattgggaac ggccgtgctg acgaatgaga agggagagat cgcaggggcg
     3361 atcatccgga aggtggcggc cggaattgcc gcgctggcgg gggactatcg gatcatcctg
     3421 gtgtcgagcg gcgccgtagg cagcgggagg gggttctttt cgggctatag gggaagtttg
     3481 gccgagcgga aggccgctgc agccgtgggg aatcctattc ttatccagca ttatcagcgg
     3541 caatttgccg catatggaat accggtagcg caggccttgt gtgaacggca gcatttctcg
     3601 agccggccgc gttttttgca gttgaaggag acctttgccg aattctggaa gaacgggatc
     3661 ctgcccatca tgaacgagaa cgacctggtg agcaacgtgg agttgaagtt ctccgacaat
     3721 gacgagctgg cgacactgac tgccatcgga ttcgatgccg aagctttgat cctttgtact
     3781 tcggtgggcg gattcatgga tgggcagggg aagatcctgt ccgaagtgga gaaagtggat
     3841 agccggatca tgggttttgt tcaaacggga aagagtgcgc ttggattggg ggggatgttg
     3901 tcgaaactga catttacgaa gctgtcgaca tcgctgggta tccgcgtcat catatgcggc
     3961 atcgggggga agaccccttt ttccgatgcc ctggccggcc ggaccgggac taattttatt
     4021 cctcaggcgt cgaatctgaa ggcgcggcag aaatggctgg ccagcggatc catcaccttc
     4081 gggagtcttt ttgtagacag aggcgcggca agggccttaa tgcagcgcag gagcctgctg
     4141 accgtgggtg tcaggaaagt acaggggaaa ttcggtgcgg gagaggtcgt gcagctggtg
     4201 gatgaagagg agaatattgt cggagtcgcc aaagtgagga taggttcggc ggagatcacg
     4261 gccggattgt cgcagaagaa tgtcatggct gcgcatgcgg atgatattgt agtcttttaa
     4321 atgaaaccga gcaaattgcg aggttccatt cgaccattgg atagacattg tgaacgaatg
     4381 aaatcgatag agcctatatt gttacgggtg cagaaggcca cgccgggtct gaggagtgcc
     4441 actgacgatc agatagcggg tgtattgcgc gcgctggcgg accttttgga atcgaacggg
     4501 gcgctgatcc tgcgcgccaa tacgcgtgac ctgaagcgtc agaaaccggg tgatccccgg
     4561 aatgaccggc tgttattgaa tgagcagagg atcaggggga tcgccggagc gcttcgtaaa
     4621 gtgagcgggc tgcctgatcc gaccggtaag gaattagacc gccggacgct ggctaatggg
     4681 ttggaagtgg aaaggatcac ggtgccgttc ggagtcgtgg gcgccattta tgaatcgagg
     4741 cccaacgtga ccttcgatat agcggcgttg tgtttacgga gtcgtaacgg atgcgtgcta
     4801 aaagggagcc aggaggcggc cgacacgaac aaagctgcta tcgggcttat ccggaaagcg
     4861 ctgtccgaaa atgggctatc acccgactgt gtagcattgt tgcctgccga ccgggatacc
     4921 gtacagcagc tgatgaaggc tgtgcgactg gtggatctgc tgatcccgcg aggttcggaa
     4981 gggttgatcc ggcatgtccg cagcaatagc ctggtgcctg taatagagac cggggcaggg
     5041 gtatgtcatg tgtatgtgga agccgaagcc gacctggaca tggcggcaca gatcgtggtg
     5101 aatgcgaagg tgagccggcc gggcgtgtgc aatgcgatgg ataccttgct ggtggacagg
     5161 gaggtcgctc cggtgttgtt gcgacgattg gaaggacagt tgggatatca tgaagtggag
     5221 gtgtatgcgg acagggaagc attgcggttg ttgaaaggat atcggctgtt gcagcgggcg
     5281 aagcctgagg attacgaccg ggagttcctg tcgttgaagt gtgcgatcag ggtggtgggc
     5341 ggaatggaag aggcgctgga gcatatccgg gtgcattcca ccaggcattc ggaggctatc
     5401 gtttcgggaa acagggacaa atgcgaacgg ttcctgaggg aggtggacgc ggcggcagtg
     5461 tatgccaatg catcgacgcg gtttaccgac ggggaggaac tggggctggg ggcggagatc
     5521 gggatctcga cgcagaagct gcatgcgagg gggccgttcg cgctggaaaa gctggtgacg
     5581 gagaaatggg tgatacgcgg gacggggcag gtgaggggat gaggcggggg ggaggccggg
     5641 caaaaggcac aggcagaaaa tggaataatt gtcagaaaaa cgcttaattt agggggtata
     5701 aaactttcct gcatatgaat ccccttcagc ggtcgctatt tttcgttttc ctattcttca
     5761 gtatcgg
//
LOCUS       DASYSV010000389         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2509830, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000389 DASYSV010000000
VERSION     DASYSV010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2509830"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VGQ51_11960"
     CDS             <1..718
                     /locus_tag="VGQ51_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEV9037335.1"
                     /translation="IGLAYMFANIIYLYTKPIGQVVWFASLLIGYWLLLSFTSAPGFP
                     HGDLTMQGNFASYVDRTILPGRLYLGIHDPEGLMSTIPAIGTALLGILAGTMLRNGPL
                     SPAAKAGRLAIGGVISLALALAWNTVFPINKNLWTSSFVLNCGGISLLLLALFYYIID
                     VRGYKKWAFFFTVIGMNSILIYLSPHFIEWHYMTDGFFHWLGQLVGDPWNALVLVFCF
                     LAVKWVFMYILYRKKIFLRV"
     gene            complement(715..1077)
                     /locus_tag="VGQ51_11965"
     CDS             complement(715..1077)
                     /locus_tag="VGQ51_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV9037336.1"
                     /translation="MTERKTSSTNFRNQSFLEEKCSLNELLDLISKRWFSDVLFCIEE
                     GNNRFKLIKDDLKFISDTILSDRLKLLERYGLITRTDFDEIPPKVEYALTDKGEELSA
                     LLGKMCEFSDILLAEMRH"
     gene            1420..1902
                     /locus_tag="VGQ51_11970"
     CDS             1420..1902
                     /locus_tag="VGQ51_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV9037337.1"
                     /translation="MAKEHHYTTSLVWTGNKGEGTANYRAYDRDHLISAKDKPELAAS
                     SDPSFRGNPARYNPEELLVAALSSCHMLSYLHLCAVNKVVVVDYRDKAEGTMEETSDG
                     GGHFTVVILHPVITVTEEGMVETAQQLHHAASKLCFIASSCNFPVRHEPEVRVEARSA
                     "
     gene            complement(1940..2905)
                     /locus_tag="VGQ51_11975"
     CDS             complement(1940..2905)
                     /locus_tag="VGQ51_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEV9037338.1"
                     /translation="MKAIWITRKGGPEVLEVRDTPEPVPAQDEVLIRVKAAGLNRSDV
                     YSRGSRSYGNDRPEIPGLEVSGTIVSCGKDVARWRPGDTVCALVTGGGYAEYVAVPAG
                     QCLPVPDGWTSEDAASLPETVLTVWSNVWKTAGLRPGENLLVHGGSSGIGSTAIQLAV
                     AFGNPIYTTAGTDEKCRWCAALGATKAINYKTQDFQTALKDVGIDVILDMTGGDFTSK
                     NLRLLSADGRLVLINAMRGKEATIDLTEVMSRRLVITGSMLKPRSAEFKAQLTADVEK
                     QVWPLIALGALRPLIYARFPLEKAAEAQRLMESGEHMGKILLVVG"
     gene            2981..3763
                     /locus_tag="VGQ51_11980"
     CDS             2981..3763
                     /locus_tag="VGQ51_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037339.1"
                     /translation="MLRNKLSNRIFFRTMLSAFGMRPVDSNFQPGIRYFQYEIGPYRT
                     DYLLHFRREPHWELYKNEIFNKLDEFTGYDLARYLNFHYDPYEGKAEFLRFLRYETSE
                     RIRYLRKRTTSWRIKLEMVLAWVEEQERLLDRAVVDTTPVAISAGHPDKVQPNAEESD
                     LKEMSPSQIGRIEVSEERNLDRVIQLLLLLKDVTAPGQPLWPVFRRFKQNDIAAIMAQ
                     FEAFNGKQRNTIEKRIIQCRQNLPLTDPKTVRLNKALAEFFY"
BASE COUNT          826 a          987 c         1069 g          913 t
ORIGIN      
        1 gatcggtctg gcctatatgt tcgccaatat catttacctg tatacaaaac caatcgggca
       61 ggtggtttgg ttcgcctcgc tgctgatcgg ttattggttg ctgttatcgt ttacttccgc
      121 acctggcttc ccgcacgggg acctcaccat gcagggcaat ttcgcctcgt atgtcgaccg
      181 gacgatactg cccggcaggc tttacctggg tatacacgac cccgaagggc tgatgtccac
      241 cattcctgct atcggtaccg cgttgctggg tatcctggcg ggcacgatgc tccggaacgg
      301 tccattgtca ccggcggcaa aggcgggtcg tctggctatt ggcggcgtga tctcgctggc
      361 gctggcgctt gcctggaata ccgtattccc gatcaacaag aacttgtgga caagctcttt
      421 cgttttgaat tgcggcggga tcagcctgct cctgctggcg ctcttttact atatcatcga
      481 tgtgagggga tacaaaaaat gggctttctt tttcaccgtg atcgggatga actcgatcct
      541 gatctatctc tcgccgcatt ttatcgaatg gcattacatg acggatgggt tcttccactg
      601 gctggggcag ctggtcggcg atccgtggaa tgcgctcgtg ctggtgtttt gtttcctggc
      661 ggtgaaatgg gtgtttatgt atatcctgta tcgtaagaag atattcttac gggtctagtg
      721 tcgcatttcg gccagcagga tatcgctgaa ttcgcacatc ttcccaagaa gtgcggagag
      781 ttcttccccc ttatcggtaa gggcgtattc caccttcgga gggatctcgt cgaaatcggt
      841 gcgggtaatg aggccatatc tttccagcag cttgagccgg tcggagagaa tggtatcgct
      901 gatgaacttc aggtcatctt tgatgagctt gaagcggttg ttaccttcct cgatacagaa
      961 aagaacgtcg ctgaaccatc ttttactgat gaggtcgagc aattcattca ggctgcattt
     1021 ttcctccagg aaggattgat tgcggaagtt ggttgaactg gttttgcgct ctgtcataac
     1081 atcagatttt aatgattggg gcctatgcag ggtcagcaag cagttggagt gatacaaact
     1141 taatcgattg ccggcgtagc actcctccaa taaaagccta tatcgaccgg ccatactaat
     1201 tttgggcaaa gcgtgataat aagagaaata ttagtggcct cctgaaaaaa cggtggtttt
     1261 tggggagggg ttggctgaaa cttaaccaat ttctttccag attttaaccg tcggcttacc
     1321 ggcgcagggg cttttgaggc taccgaggat cccaacgatg aggacgacca tgaccgggac
     1381 aacgacgatt ttgccatgga ttcgtaattt tagggaatta tggcaaagga acatcattac
     1441 accacctccc tcgtctggac cggcaacaag ggtgaaggga cggccaatta ccgggcttac
     1501 gaccgggatc atcttatttc cgccaaagac aaaccagaac tggcggcctc ctcggacccc
     1561 tcttttcgcg ggaacccggc gcgttataac ccggaagaat tgctggtcgc ggccctttcc
     1621 tcctgtcata tgctttccta tctgcatctc tgcgcggtca ataaggtggt cgtcgtagat
     1681 taccgggaca aggcggaagg gacgatggaa gagaccagcg atgggggagg acattttact
     1741 gtcgttatac tgcatcctgt cattacggtc accgaagagg ggatggtaga gactgcgcag
     1801 cagctgcatc atgccgcgag caagctctgt tttatcgcga gttcctgcaa tttcccggtt
     1861 cggcatgagc cggaggtgcg ggtcgaggcg aggtctgcct gacgtaagtc tgccagcggg
     1921 ggatcggagc ggggtctgcc tagcccacca ccaggagtat cttgcccata tgttcacccg
     1981 actccatgag ccgctgggct tcggcggcct tttcgagcgg gaagcgggca tagatcagcg
     2041 gacggagcgc gccgagggcg atcaggggcc agacctgctt ttccacatcc gcggtaagct
     2101 gtgccttgaa ttcggcgctc cgaggtttca gcatactacc ggtgatgacc agccgccgcg
     2161 acatgacctc ggtgagatcg atggtcgctt cttttccgcg catggcattg atcaatacca
     2221 accggccatc ggcgctcaaa agccggaggt ttttggaggt gaaatcaccg cccgtcatat
     2281 cgaggatgac gtcgataccg acatctttta acgccgtctg aaaatcctgt gtcttataat
     2341 taatggcctt tgtcgccccg agggctgcgc accaccggca tttttcatcc gtgccggcgg
     2401 ttgtatagat ggggttaccg aaggcaacgg ccagctgaat ggccgtggaa ccgatgccgc
     2461 tgctgcctcc atggacgagg aggttttcgc cgggacgaag ccccgccgtc ttccagacat
     2521 tggaccatac ggtcaggacc gtctcgggca gggatgcggc atcttcggat gtccagccat
     2581 ccgggacagg gagacactga cccgcgggca ccgcgacgta ttcggcataa ccgccaccgg
     2641 tgacgagcgc gcagacggta tcgccgggcc gccagcgggc aacgtctttt ccacaggaga
     2701 cgatggtccc ggatacttcc agaccgggta tctcaggccg gtcgttacca tatgacctgc
     2761 tgccccggga atagacatca ctgcggttga ggccggcggc cttcaccctg atcagcacct
     2821 catcctgtgc ggggacgggt tcgggcgtgt cgcggacctc cagtacctcg gggccgcctt
     2881 tgcgggtgat ccatattgct ttcattgcat ttgaaaaata ctaaattagg acgtttcagg
     2941 aggattgttt tggttaaatt gtcgctttaa gccttagact atgcttagga ataaattgag
     3001 caaccggatc tttttccgga ccatgttgtc cgctttcgga atgcgtccgg tggactcgaa
     3061 cttccaaccg gggatccgct attttcaata cgagatcggc ccgtatcgca ccgattatct
     3121 tcttcacttc cggcgggagc cgcactggga gctgtataag aatgagatct tcaacaaatt
     3181 agatgagttt acgggatatg acctggcacg ttatctgaac tttcattacg atccgtatga
     3241 aggcaaggcc gaattcctgc ggttcctccg ttatgaaacg agcgaacgca tacggtatct
     3301 gcgcaaaagg acgaccagct ggcgcatcaa gctggagatg gtactggcct gggtggagga
     3361 gcaggagcgg ttattggaca gggcggtggt tgacaccacg cctgtcgcga tatcagcagg
     3421 acatcccgat aaggtccaac cgaacgcaga ggaatcagac cttaaggaga tgtcgccgtc
     3481 ccagatcgga cggatcgaag tgtctgaaga acgcaacctt gacagggtca tccagctgct
     3541 attgctgttg aaagatgtga cagcgccggg gcaaccgctt tggcccgtgt ttcggcggtt
     3601 caaacagaac gatatcgctg ctatcatggc acagttcgaa gccttcaatg gtaaacagag
     3661 gaacactatc gagaaaagga ttatccaatg cagacagaac ttgcctctca ccgaccccaa
     3721 aaccgtccgg ctgaataaag ccctggccga attcttctac taatgcggac tggctcccca
     3781 tccatgacgc atcca
//
LOCUS       DASYSV010000390         7577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_717381, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000390 DASYSV010000000
VERSION     DASYSV010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717381"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4245)
                     /locus_tag="VGQ51_11985"
     CDS             complement(<1..4245)
                     /locus_tag="VGQ51_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037340.1"
                     /translation="MAGSSNDTVFINPSPENSGFPPYLDFTTLRTNAINYLGPITGKY
                     WTDYNVHDPGITTLDVLMFAILDLGYRTNLPIASLLAPGSAADAGSGIAGASLATGPG
                     TASGTKPVSIAHHRSWRHGHSSAAGGAPAADTNFFTPAQVLGCNPTSILDYRKLLMDL
                     EEVRNAWLEPMKASQTKYNGLYKIYLETEKDEADFSTSGEWHKYKQAVRLEVQKTLHA
                     FRNLCEDFEKIVFLKRVHFGVNMDIEIVPGTDTGVAYQALVAALFDFFSPVPTFYTLG
                     QLTAKDVSLATIFEGRPYTGRPSHGFLLDEDMPDRPSGDQTISKSVLVQTLLGLLGVA
                     GINTIKTIRSLELLDENGDVMSSTGGKWEYPLPPDSLSAFSLSVSTFRWFQNGQLLST
                     QLGSYNAVLVMNSLHSGKVIYPAGSAALDAAAPYGSPLAGLGDYNSIQNDFPQVYGIG
                     VGGLPPTASALRQSQAWQFKGYLLFFDQLLADYLAQLSNIRQVFAMNAPSGGSTSFGG
                     NLGSVPDLDMLLRFPPSDAAGGATLLFPVAAKDWKPLVTAKTEDPGAQGGTSTAKVNA
                     PVPAAKIDALSPYTFGSSVERDVALATTIVLFSSAQLTVQTIQLDSGGWKYYLAGTGD
                     VFVLVSKNSFTNKAGALGEAATVLYIGGYKENYTLVSLGEISSYTFNLAASGAAYYQY
                     LQSILEKPQEYTERRTAFVEHLLARFSETFADYALLESGFASPATIGQNQLELMQRYL
                     SKWPELSADRGKAFDLRMDGWRSGNISGLEKRFKAECGIAELRRHDLCHFEVQKMDER
                     FHIAFTLAEEELFTCSEPLKYDEAVPAVSSLFTALSDRDNYRIIHSTAAFGYRVEVDF
                     YGSLTARSVRIWPDHAGAATAVDTLYRMLQISPSKGDSVISEYQYRAQLLDHQGRIMR
                     TAIDGFIREQEAFEYGIHHLGRINDEGIWTPDQPVPIGPLYPNSDPEPGSRRWIDIDG
                     FKIYPRQNVLGRGKADRWSFEVLDGDNSFRFESTRDFDTESAAIEASYQLLHFLCETQ
                     YYAIRQEHEDWYKIVIRVGDVIWANGEPDYDSESSARERIPLMVSQARLHLYRLDVIS
                     RPNQWKFLFYLGMPERGSMVFESVQSFGTQHEAVAAARVFHDAGPDWRWRESEEGFYL
                     ETGLPDGERLVCFHAGGVGVHSGGSSAHLRYLLETKEGIYRLAAGDEELARRMVIPDE
                     TSREGAYIYRLVDKDHPWAVHPMMRHETRPEAEALRDRLIAKAVQGYVYLEISLGDDD
                     IRTGSTTNGAQKFYYRLKCRNNYFSRIGIPAHGGKLVLFESVDGFDDGDKAQTAFMQT
                     YLSILQKATDPENYGDGKYIALSDRDSIPTDDDHKPVVLVPAETREAFLQAGLDVVDE
                     LVKAALSYPIRLADHATRYRFILGNEGHTDWTSVRDY"
     gene            complement(4246..>7577)
                     /locus_tag="VGQ51_11990"
     CDS             complement(4246..>7577)
                     /locus_tag="VGQ51_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037341.1"
                     /translation="QVPNYPLPAGLSLKDGKDSKSGDIFFALSDGITVTQTQTTSFRT
                     LFVNTPTVSDVSYVEGVYMAPDATKADGVDQAFPNPSTASWPTLGAKVSKYTPPMSPG
                     PIDYPTARLGFILASKVLLMAEGVRKVHIRLACRYTTRCSTAGLSDEMAQLFMSYFAS
                     GWLIITQELIDRAVILGMDKKKGDKIRDHYLKDRCREKICADDDQPYYLPFFLIEIKA
                     RIEKYIIAFREGVEMIAEHKDSLRAEWMRFLEEEGKRFDMEVFELDIWKSLVMPQPVF
                     QLAFSGPKGWVEPAHIAMDMVPDAVSGDFQLNLYALLEKDQDAVTWADKKALKEDFGT
                     TDPLVKVQLNDTIKIPLEDFLEAAGVSAGRASDDCCLKNLQSVCGGLVSFYTFFRNVI
                     VLGEGTHIRVTVCGVKNLVVQNDDNVLSAKKPFTPFGVKPVVPDFNTLLAPPDHTRPN
                     SYNLIGPNFYIGSAEVFLKRWHRINVNINWKGVPRSFWNYYSSYIKHQHAWHHHHHFP
                     KHQVNLALLHNGIWDRETDHPHLHHNLFTKHYNRHFFAHSDENIGGCRDKDEYRHTFH
                     LRPDDFGLEGQLSYDPTFAPLTSFSNGVLNGYLRLTMENQDFLHKLYPIVMADKMIAR
                     AKRTTAPLPALPNEPWTPTILDIAIDYEAGANSDEMQLIQLYPYDGTYYKADINGQPP
                     LFATFCDEGNLFIGLSGLVPGDGLNILFQLAEATADTEDGSVKVNWQYLASNIWESLR
                     TGFEVLQDNTNGLSTTGIVQYSFPKDITNDNTVMPAGTWWLRASVTGGAEATSQTMAI
                     ITQAALAGFVNNIQLNDQSRPGNIPLASGSLTKLTDPDPNVQSVSQPYASFGGSAPEA
                     SGSAYNIRVSEQLRHKGRAIQKWDYERIVLQQFPKLLRAKCINHSYALSNQRYRWDFP
                     MAPGNIILAVLPDPAQLVVADSLQPTAPMSMLTSIEEFLAEIKSPFVRVFARNPRYEP
                     IDMCLKVVLQPDMNPKFYAARLETDIRSFMAPWKGNTAAFQFAQRFYSADLVNYIQGL
                     DYIDKLLHLEMAHQGSPMKDPIPDYLDPLTPRSILVAGKIVIHVVAATRKPFSAGGKT
                     GSTTSKKEPAINPIILRSIKK"
BASE COUNT         1627 a         2153 c         2075 g         1722 t
ORIGIN      
        1 gtagtcgcgg acacttgtcc agtccgtgtg accttcgtta cccaaaatga aacggtaacg
       61 cgtcgcatgg tcggcgagcc ttatcggata ggacaaggca gcctttacca actcatctac
      121 cacatcgaga cctgcctgca ggaaggcttc gcgcgtttcg gctggtacga gtaccaccgg
      181 cttgtgatca tcgtccgtcg ggatcgaatc ccggtcgctc aaagcgatgt atttcccatc
      241 tccataattt tcgggatcgg tcgccttctg caggatggac aggtaggtct gcatgaaggc
      301 tgtctgagcc ttatcgccat cgtcgaaacc gtccacactc tcgaacaata ccagcttgcc
      361 gccatgcgcc ggtatgccga tgcgggagaa ataattgttg cggcacttca gccggtaata
      421 gaatttttga gcgccgttgg tcgtcgatcc tgttcgtatg tcatcgtcgc ccagactgat
      481 ctcgagatag acatatccct gcaccgcctt cgcgatcagc cgatcccgga gcgcttccgc
      541 ctccggccgg gtttcatgac gcatcatcgg atgcacggcc cacggatgat ccttgtccac
      601 cagccgatat atataggctc cttcccgcga ggtctcatcg gggatgacca tgcgacgcgc
      661 cagctcctca tcccccgctg ccaatctgta aataccttct ttggtctcca gcaaatagcg
      721 caaatgggcg gaggatccgc ccgaatggac cccgacgccg ccggcatgga aacagaccag
      781 gcgttcgcca tcgggcagcc cggtctcgag gtagaaccct tcttcagact cgcgccatct
      841 ccaatccggc ccggcgtcgt ggaagacgcg cgccgccgcc actgcctcat gctgcgtccc
      901 gaaggactgc acgctctcga aaaccatgct ccctctttcg ggcataccca aatagaaaag
      961 gaatttccac tggtttggcc gggagatcac atccaggcga tacaaatgca gccgcgcctg
     1021 cgagaccatc agcggtatcc gttcgcgcgc gctcgattca ctgtcatagt ccggctctcc
     1081 attggcccaa atgacatctc cgacacggat cacgatctta taccaatctt catgttcttg
     1141 ccggatggca tagtattgtg tctcgcacag gaaatgcaat agctgataac tggcttctat
     1201 ggccgctgat tccgtatcga aatcccgggt ggattcgaac cggaagctgt tgtccccgtc
     1261 gagcacctcg aagctccacc tgtcggcttt accacggccc agtacgttct ggcggggata
     1321 gatcttgaag ccgtcgatat ctatccaccg gcgcgaacct ggctccggat cgctgttggg
     1381 gtatagcggg ccgataggaa ctggttgatc gggtgtccat atcccttcat cgttgatgcg
     1441 gccgagatgg tggatgccat attcgaaggc ctcctgctcc ctgatgaatc cgtcgatggc
     1501 ggtgcgcatg atgcgaccct gatgatccag caactgcgcg cggtactggt actcgctgat
     1561 cacgctgtcc ccttttgacg gcgatatttg caacatccgg tacagggtat cgaccgccgt
     1621 cgccgcaccc gcatgatccg gccatatcct gaccgatcgg gccgtaaggc taccataaaa
     1681 atccacttcc acacgatagc cgaatgctgc cgtgctgtga atgatccggt aattgtcgcg
     1741 atcggacaac gcggtgaaaa gcgagctgac ggccgggacg gcttcgtcat atttcaacgg
     1801 ctcgctacag gtgaagagct cttcctccgc cagggtaaag gcgatatgga aacgctcgtc
     1861 cattttctgc acctcgaaat gacagagatc atgccggcgc agttcggcga tcccacactc
     1921 tgccttgaac cttttttcaa gaccgctgat attgccgctt ctccatccgt ccatccgaag
     1981 gtcgaaagcc tttcccctgt ctgcgctcaa ttccggccat ttgctaaggt accgctgcat
     2041 caattccagc tggttttgcc cgatggtcgc cggcgacgcg aacccggatt ccagcagggc
     2101 atagtcggcg aaggtttctg agaatcgtgc cagaagatgt tctacgaatg ccgttctccg
     2161 ctcggtatat tcctgcggtt tctccaaaat ggactggaga tactgatagt aggcggcgcc
     2221 gctcgccgcc aggttgaagg tataggagct gatctcgccc agcgagacca atgtataatt
     2281 ctctttatag ccaccgatgt acaggacggt ggccgcctcg cccaatgcac cggccttgtt
     2341 ggtaaaagaa ttcttgctca ccaggacaaa gacgtccccg gtgccggcga gataatattt
     2401 ccatccgcca ctatccagct ggatggtctg cacggttagc tgcgccgagg agaaaaggac
     2461 gatggtcgtt gcaagtgcaa catctctctc cactgatgat ccgaaggtat agggggaaag
     2521 tgcgtcgatc tttgccgccg gcaccggtgc attcaccttc gcggtggatg ttcccccctg
     2581 agcgcccggg tcttccgttt tggccgtgac aaggggcttc caatctttgg cggccactgg
     2641 gaataacagc gtggctcctc cggccgcatc ggacggaggg aaacgcaaca gcatatccag
     2701 atcgggcacc gagcccaggt ttcctccgaa cgaggtagac ccgccggacg gcgcattcat
     2761 cgcaaacacc tgccggatat tactcagctg tgccaggtag tccgccagca gctgatcgaa
     2821 gaacaataga tagcctttga attgccaggc ttgcgattgg cgcagggcag aggccgtggg
     2881 aggcagtccg ccgacaccga tgccatacac ctgcggaaaa tcgttctgga tagaattgta
     2941 atctcccaga cctgctaacg gcgagccata gggtgctgcc gcatccagtg ccgccgatcc
     3001 cgccggatag atcacttttc cggaatgaag tgagttcatg accaacacgg cgttgtagga
     3061 acccagctga gtcgaaagca gctgtccgtt ctgaaaccag cggaaggtgg acacggatag
     3121 cgaaaaggca gacaatgaat cgggcggtaa aggatattcc cattttccgc cggtggagct
     3181 catcacgtcg ccattttcat ccagcaattc cagcgaccgg atggtcttga tggtgttgat
     3241 gcccgcgact ccgagcaatc cgagcagcgt ctggaccagc accgattttg agatcgtctg
     3301 atcaccggag ggacgatcgg gcatgtcctc gtcgagcagg aacccgtgac tgggacgtcc
     3361 cgtataaggc cggccctcga atatcgtggc gagcgaaacg tcttttgccg tcagctgacc
     3421 cagcgtataa aatgtcggga cgggcgagaa aaagtcaaat aatgcggcga caagggcctg
     3481 gtaggcaacg ccggtgtccg tccccgggac gatctcgatg tccatgttga cgccgaagtg
     3541 tactcgcttc aggaaaacta tcttttcgaa atcctcgcac agattacgga acgcatgcag
     3601 cgtcttttgc acctccagac gaaccgcctg cttgtattta tgccattcgc cggaagtgga
     3661 aaaatcagct tcgtcttttt cggtctcgag atatatctta taaagaccgt tgtatttcgt
     3721 ctgactcgct ttcatgggct ccagccatgc gttgcgcacc tcttcgagat ccatgagcaa
     3781 tttgcggtag tcgaggatcg atgtcggatt gcagcctaat acctgggccg gcgtgaaaaa
     3841 attggtatcg gctgccggag caccgcccgc cgccgatgaa tgcccatgcc tccacgaacg
     3901 atgatgcgcg atcgagacgg gcttcgtccc cgaagccgtc cccgggcccg tcgccaaaga
     3961 tgccccggca attccggaac ccgcatccgc ggccgaaccc ggcgccagca agctggcaat
     4021 tggcaaattg gtccgatagc caagatcgag tatcgcaaac ataaggacat ccagcgtagt
     4081 gatacccgga tcatgcacgt tataatccgt ccaatacttt ccggtgatag gcccgagata
     4141 attgatggca ttcgtacgca gcgtcgtaaa atcgaggtac ggcgggaacc ccgaattttc
     4201 cgggctgggg ttaataaaga cggtatcgtt actgcttcct gccatttatt tctttattga
     4261 tcgcaagatg atggggttga tcgccggttc ttttttgctt gtggtcgatc ccgttttacc
     4321 gccggccgaa aagggtttgc gagtggccgc gaccacatga atgacgatct tgcccgcgac
     4381 gagtatcgac cgcggcgtca gcgggtcgag atagtcgggt attggatcct tcatcggtga
     4441 gccctggtgc gccatttcga gatgcaacag cttgtcgata taatccagcc cctggatata
     4501 attgacaaga tcggcgctat agaaccgttg cgcgaactga aaggcggccg tgtttccctt
     4561 ccatggcgcc ataaagctcc ggatgtccgt ttccagccgg gccgcataaa attttggatt
     4621 catatccggc tgcaacacca cctttagaca catatcgatg ggctcgtaac gcgggttacg
     4681 cgcgaatacc cgtacgaaag gtgacttgat ctccgccaaa aattcttcga tactggtaag
     4741 catgctcatg ggcgccgtag gctgcagcga atcggccacg accagctgcg ccgggtcggg
     4801 taagacggcg agaatgatat tgcccggcgc catcgggaaa tcccagcggt accgctggtt
     4861 gctaagcgca tagctatggt ttatacattt ggcccgcagg agttttggaa attgctgcag
     4921 gactatccgt tcgtagtccc atttctgtat cgccctcccc ttgtgacgca attgttcgct
     4981 gacccttatg ttgtacgcag aacccgaggc ctcgggcgcg gaacctccga atgaggcata
     5041 tggctgagac acgctttgca cattgggatc cggatcggtc agcttggtaa gcgaccccga
     5101 cgcaagcggt atattccccg gccgcgattg atcgttgagc tgaatattgt tgacgaagcc
     5161 ggcgagcgcg gcctgcgtga tgatcgccat cgtctgactg gtagcttcgg cgccgccggt
     5221 gaccgacgcc ctaagccacc atgtgccggc cggcatgacg gtattgtcgt tggtgatatc
     5281 cttggggaaa ctgtactgaa cgatcccggt agtcgacaat ccgttggtat tgtcctgcag
     5341 gacttcgaaa cccgtccgca gcgattccca tatattgctg gccaggtatt gccaattgac
     5401 tttcaccgat ccgtcttccg tatccgccgt cgcttcagcc agctggaaca ggatgttcag
     5461 cccatccccg ggcaccagac cggaaagccc gatgaataag ttgccttcgt cgcaaaaagt
     5521 ggcgaacagc ggcggctgcc cgttaatatc tgccttgtag tacgtcccgt catagggata
     5581 cagctgtatc agctgcatct cgtccgaatt cgcacccgcc tcataatcga tggcgatgtc
     5641 cagtatcgtc ggcgtccagg gctcgttggg cagtgcgggc agcggtgccg tcgttctttt
     5701 ggcccgcgcg atcattttgt ctgccattac aatgggatag agcttgtgaa ggaaatcctg
     5761 gttctccatg gtcagccgga gataaccgtt aaggacgccg ttgctaaagc tggtcagggg
     5821 ggcaaaggtg ggatcgtagc tcagctgacc ctccagaccg aaatcgtccg ggcggagatg
     5881 gaaggtgtgc cggtattcgt ctttgtcgcg acagccgcca atgttctcgt cactatgtgc
     5941 aaagaagtgc cggttgtaat gtttggtgaa caggttgtgg tggaggtggg gatgatccgt
     6001 ctccctgtcc cagatgccat tgtgaagcaa cgcgaggttc acctggtgtt tcgggaaatg
     6061 gtgatgatgg tgccaggcat gctggtgttt gatataagag ctgtaataat tccagaacga
     6121 tctcggaacg cccttccagt tgatattgac gttgatccgg tgccatcttt tcaggaacac
     6181 ctcggcgctg ccgatataga aattggggcc gattaggtta tagctgttcg gcctggtatg
     6241 atccggcggc gccaacagcg tgttgaagtc ggggaccacc ggctttacgc caaacggcgt
     6301 gaaaggcttt ttggcgctca gaacattgtc atcgttttga acgacgagat tttttacgcc
     6361 acaaacggta acacgaatgt gggtcccctc accgagcacg atcacattgc ggaaaaaggt
     6421 atagaaggag accaacccgc cgcagacgga ctgaagattc ttcagacagc agtcatcgga
     6481 tgctctcccc gcgctcacgc cggctgcctc gagaaaatct tccagcggga tcttgatggt
     6541 atcgttcaat tgcaccttca ccagcggatc ggtcgtaccg aaatcttctt tcaatgcctt
     6601 tttgtcggcc catgtgacgg catcctgatc tttttcgagc agggcataga ggttcagttg
     6661 aaaatccccg gaaaccgcat ccggaaccat gtccatcgct atatgtgcgg gctcgaccca
     6721 tcccttcggc ccgctgaacg ccagctggaa cacaggctgc ggcatcacga ggctcttcca
     6781 gatgtccagc tcgaagacct ccatgtcgaa acgcttgcct tcttcttcca ggaatcgcat
     6841 ccactccgct ctgagactgt ctttatgctc ggcgatcatc tccacgcctt cacgaaatgc
     6901 gatgatatat ttttcaatgc gggcctttat ctctatcaga aagaaaggca gataataagg
     6961 ttggtcgtca tccgcacaga tcttctcgcg gcaacgatcc ttgagataat gatcgcggat
     7021 cttgtcgccc tttttcttgt ccatccccag gatcaccgcc cgatctatca attcctgcgt
     7081 gatgatcagc cacccggagg cgaaatagct catgaacagc tgggccatct catcgctgag
     7141 accggcggtg ctgcagcgcg tcgtataccg gcacgcaagc cggatatgca cctttcggac
     7201 gccttcagcc atcagcaaca ctttcgaggc gagaatgaaa cccagacggg cggtaggata
     7261 atcgatgggc cctggagaca tcggcggggt atatttgctg acttttgcac ccagtgtcgg
     7321 ccagctcgcg gtcgatggat tgggaaatgc ctggtcgaca ccgtccgcct tggtcgcatc
     7381 cggggccata tatacgccct ccacatagga gacgtcggac accgtcggcg tattgacgaa
     7441 taaggtgcgg aaactggtgg tctgcgtttg cgtcaccgta ataccatcgg aaagggcaaa
     7501 aaagatatcg ccgctcttgc tgtctttgcc atccttgagc gacagacccg ccggcagggg
     7561 ataattcggt acctgtt
//
LOCUS       DASYSV010000391         4468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_334426, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000391 DASYSV010000000
VERSION     DASYSV010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334426"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VGQ51_11995"
     CDS             <1..391
                     /locus_tag="VGQ51_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEV9037342.1"
                     /translation="EAFPDMPRHTVPTALQGPRAERVYELRSYEGPTEKYFANKVQMF
                     NEGGEMPLFQRLGFNAVFYASVLSGSHMPNLMYMTSFENMASREQHWKTFSADPAWKQ
                     LSSSPQYQHNVSHIDIVFLHPTEYSDL"
     gene            601..1098
                     /locus_tag="VGQ51_12000"
     CDS             601..1098
                     /locus_tag="VGQ51_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV9037343.1"
                     /translation="MDSSTDTMFIRPIRPEDNPALAEIIRNAFREFNLAKPGTAFYDK
                     ALDDLYATFQSPRSRYHVGLADGQVAGGGGIFPSEGLPPTICELVKMYLVPSVRGKGL
                     GSLLIDECLRSARQFGYRQVYIETMPEFGKAVGLYEKLGFRHLDGALGNTGHYTCSVW
                     MLLDL"
     gene            complement(1160..1780)
                     /locus_tag="VGQ51_12005"
     CDS             complement(1160..1780)
                     /locus_tag="VGQ51_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEV9037344.1"
                     /translation="MSKKEAPIDYLRRWIPENAAGLALEYLNHYHVHLTITRERKSVL
                     GDYRHATRSSNHRISVNGNLNTYSFLITLIHELAHLVTFMEYGNGVASHGKEWKAIYR
                     KMLEEFIRLGVFPTDILAALKKSLHNLPASSCADEDLMRILKRYDTDGGGLLLVEQVA
                     EGTCFLLEDKRIFRKGKKLRKRYQCVEVATGKLYLFSPIYEVKPAS"
     gene            complement(1773..2177)
                     /locus_tag="VGQ51_12010"
     CDS             complement(1773..2177)
                     /locus_tag="VGQ51_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037345.1"
                     /translation="MENKKVRRALSFFVLIFVVFNLLAIFHVFPVEWKMDYRVLLIGN
                     SLLFGVTYVSFLLHIKGLRNPNPHVFVRTMYGSLLVKMLICLAAVMIYAMASGGMINR
                     NGIIACFVLYLIYTVLEVRVLQQLYKKRPKNV"
     gene            complement(2167..2466)
                     /locus_tag="VGQ51_12015"
     CDS             complement(2167..2466)
                     /locus_tag="VGQ51_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037346.1"
                     /translation="MPKPPSDSEGEGLRREDGEFSRKGGDDGDGASRRRQLLRYAGIS
                     SEVVASVGVSVFLGMKADKGLKVSFPIFSWALPLLVIVVLLVQLVKAGSGKKDGK"
     gene            complement(2444..2896)
                     /locus_tag="VGQ51_12020"
     CDS             complement(2444..2896)
                     /locus_tag="VGQ51_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV9037347.1"
                     /translation="MFNGKTKSNDFGLGEESVSTSASLIGGGTTMKGDITSNGDLRID
                     GTLVGNINCTAKVIIGANGNVQGDVNGQQADILGKVTGTIKVKDLLQLKGGCTVNGNL
                     QAAKLQIEPSANFNGECHMTAVGAAVPANGKTSPEVKKEFNHAQAALG"
     gene            complement(2913..3308)
                     /locus_tag="VGQ51_12025"
     CDS             complement(2913..3308)
                     /locus_tag="VGQ51_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037348.1"
                     /translation="MAKFDANSTLKRLQNRYRLVVMNDDTYEEVVTFKLSRLSVYTVL
                     CTIFVLLTGLTVALIVFTPLKLYIPGYGDVSNTRDLRELKMRTDSLELEVRYKDKYLE
                     SIRSVLQGGSTVKLDTTTLKVPKPERVND"
     gene            3396..>4468
                     /locus_tag="VGQ51_12030"
     CDS             3396..>4468
                     /locus_tag="VGQ51_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037349.1"
                     /translation="MLLGIFSCFYLAVAAQVGVDYDLVKPKKFENRTLASETSNDGKK
                     FKKSRRFIQNTITHYNYYFNANQKLQLILTLAKARFRDDYTKLLPFYNYTLDATAGQK
                     KDLDSIIYKATMGILIHDTRNDWIDNLYLLMGEAYYFKKDFDSAYITLQFINWAFAPR
                     DKDGYYLPIGSNENEMQGDNASVVSTKEHPNVLQKTFSMPPSRNDALVWKIRTYIAQE
                     DYASAASLIQILLHDPQFPPRLNPGLNEVRALWYYQQGINDSAAIFLEKALPAAENRE
                     EVARWEYLIAQLYEKTGNSFEAKTFYERTVTHTYNPVLEIYARLNAIRQNKEGGEDYI
                     AKNIEALIKMAHKDRYAAYRDII"
BASE COUNT         1166 a         1342 c          937 g         1023 t
ORIGIN      
        1 cgaggccttt cccgatatgc cccggcacac cgtacctacg gctttacagg gcccccgtgc
       61 cgaaagagtg tatgagctgc gcagctatga aggtcccacc gaaaaatatt tcgccaacaa
      121 agtacagatg ttcaacgaag gaggtgagat gccgcttttt caacgtctcg gtttcaatgc
      181 agtcttttac gccagcgtcc tttccggcag ccatatgccc aatctgatgt acatgaccag
      241 cttcgaaaat atggcctccc gtgaacaaca ctggaaaaca tttagcgcag accctgcctg
      301 gaaacaatta tcctcctccc cccaatacca gcataatgtg tcgcatatcg atatcgtttt
      361 cctccatccc accgaatact cggatctctg accggccact gcacactcgg cgcggtccct
      421 ctaaaaaacc ttttcacaca tccatactaa cccccccgcc gcctgcgtta accaccaaat
      481 atcctatcta tgaagcacgc agacaagcca tttatcggca tccttatcgt caccatcgcc
      541 atcctgtacg gcgtcatcgc cctcctcagc tgatccgtcc cttttcggta ttttcgccga
      601 atggacagct ccactgacac tatgtttatc cgaccgatcc ggccggagga caaccctgca
      661 ttagccgaaa ttatccgcaa cgccttcaga gaattcaacc tggccaaacc cggcacagcc
      721 ttctatgaca aggccctcga cgacctatac gccacctttc agtcaccccg cagccggtat
      781 catgtcggtc ttgccgatgg tcaggtcgcc ggcggaggag gcatctttcc ttccgaaggt
      841 ttaccgccaa ccatttgcga actggtaaaa atgtatctgg ttccatccgt cagaggcaaa
      901 ggcctgggaa gcctgctgat cgatgaatgt ctgcgatctg cccggcaatt cggctatcgc
      961 caggtataca tcgagactat gccggaattc ggaaaagccg ttggcttata cgaaaaactg
     1021 ggcttccgcc atctcgacgg agcccttgga aatacggggc actacacttg ctctgtgtgg
     1081 atgcttctcg acctctgaac aagcacccag cacgaacaaa acaggatgga tcaaaaacgg
     1141 gcaacgatca accaggcgct catgaagcgg gtttcacctc atatatcgga ctgaaaagat
     1201 acaattttcc tgttgctacc tccacacact gatatctttt tcgcaatttt tttcctttcc
     1261 ggaatatccg cttgtcctcc agcagaaaac aagtcccctc cgccacctgc tccaccaaca
     1321 gcagaccccc gccatcggta tcataacgct tcaatatccg cataaggtcc tcatcggcac
     1381 aactgctcgc cggcagatta tgcagactct ttttcaacgc agcgagaatg tcggttggaa
     1441 aaacacccaa tcgtataaat tcctccagca tttttcgata aatagccttc cactcctttc
     1501 catgcgaagc caccccattg ccatattcca taaaggtcac caggtgcgcc agctcatgga
     1561 tcagggtgat caggaaagaa taggtattca gattgccgtt gacgctgatc ctgtgattgc
     1621 tgctccgcgt ggcatgccgg taatccccca gaacactttt ccgctcccgc gttatcgtca
     1681 gatgtacatg gtaatgattg agatactcca atgccagccc ggccgcgttt tcagggatcc
     1741 aacgacgcaa ataatcgata ggagcttctt tcttagacat ttttcggcct cttcttatat
     1801 agctgctgca acaccctcac ttccaacaca gtataaatca gatacaggac aaaacaggcg
     1861 atgatgccgt tgcgattgat cattcccccg gacgccatgg catagatcat caccgccgca
     1921 agacagatca gcatctttac cagcagactt ccatacatcg tcctcacgaa gacatgtgga
     1981 ttcgggttac gcaacccttt gatatgcaat agaaaagata cgtatgtcac cccgaacagc
     2041 agactgttcc cgatcaggag tacccgatag tccattttcc actccactgg aaatacatga
     2101 aaaatggcca ggaggttgaa aaccacgaag atcaatacga aaaaggagag tgctctccga
     2161 acttttttat tttccatctt tcttacccga acccgctttg accagctgaa ccagcaggac
     2221 cacgattacc agcaaaggta acgcccagga aaagatcgga aaagaaacct ttagcccttt
     2281 atccgccttc atccccagga atacggaaac cccgacggag gcgaccacct ccgaggaaat
     2341 acccgcatac cgcaatagct gccgtcgacg actggcacca tcgccatcat cacccccctt
     2401 tcgggagaat tcgccgtctt cgcggcgtaa cccctcccct tcgctatccg agggcggctt
     2461 gggcatggtt gaattctttc ttgacctccg gggacgtctt gccattagcc ggaacagcag
     2521 cacccactgc ggtcatatga cattcgccgt tgaaattggc cgaaggctcg atctgcagct
     2581 tggcagcctg gagattgcca ttgacggtac agccgccttt cagctgcaac agatccttga
     2641 ccttaatggt tccggtaact tttccgagga tgtccgcctg ctggccattg acatcgccct
     2701 ggacattgcc attggcgccg atgatcacct tggccgtaca attgatattt cccaccagcg
     2761 tgccgtcgat acgcagatcg ccgttgctgg tgatgtcccc tttcatggtc gtgcccccgc
     2821 cgatcaggct ggcactcgtg ctcacggatt cttctccgag cccaaaatca ttggattttg
     2881 ttttgccgtt aaacatataa tatggatttt agttaatcat taaccctctc gggcttcggt
     2941 accttcagcg tcgtcgtatc cagcttcaca gtcgaccctc cctgcaacac actccttata
     3001 ctctccaaat atttatcctt ataccttact tccagctcta acgaatcagt acgcattttt
     3061 agttcacgga gatccctcgt attgctcaca tccccatatc ccggaatata cagcttcagc
     3121 ggcgtaaaaa cgatcaacgc caccgtaagc ccggtcagca gtacaaaaat cgtgcagaga
     3181 acggtataaa cactaagacg ggaaagcttg aaggtcacca cctcctcgta ggtatcatca
     3241 ttcatgacca ccagtcggta ccggttttga agacgtttga gggtggaatt ggcatcgaat
     3301 ttggccatat tggacttatt agcataaaga tagcaaaata aatcacggga aaaagagtta
     3361 atacctttat ttgcagcgtg gcgtatagta agtacttgct attagggatt ttttcatgct
     3421 tttatctggc cgtcgcggcc caggtaggtg tagactatga cctcgtaaag ccgaaaaaat
     3481 tcgagaaccg caccctcgcc tccgaaacct ccaacgacgg caagaaattc aaaaaaagcc
     3541 gacgcttcat ccagaatacc atcacccact ataactatta cttcaacgcc aaccaaaagc
     3601 tgcagctcat cctcaccctg gccaaagccc gttttcgcga cgattatacc aaattgctgc
     3661 ccttctacaa ctacaccctc gacgccacgg cgggtcaaaa aaaggacctc gattccatta
     3721 tttacaaggc gaccatgggc attcttatcc atgacacacg caacgactgg atcgacaacc
     3781 tctacctgct catgggcgaa gcctattatt tcaagaaaga cttcgactcc gcctatatta
     3841 cgctccaatt catcaactgg gccttcgccc cgagggataa agatggctac tacctgccca
     3901 tcggcagcaa cgagaacgag atgcagggcg ataatgccag cgtcgtcagc accaaagaac
     3961 accccaacgt ccttcaaaag acgttttcca tgccccccag ccgcaacgat gcgctcgtct
     4021 ggaagatccg cacttacatc gcccaggaag actatgcctc agcggcgagc ctgatccaga
     4081 tccttctcca cgacccacag ttcccgccgc ggctaaaccc cggcctgaat gaggtcaggg
     4141 ccctctggta ttaccaacag ggcatcaatg acagcgccgc cattttcctg gaaaaagcgc
     4201 tgccggccgc cgaaaaccgc gaggaggtcg cccgatggga atacctgatc gcccagctct
     4261 atgaaaagac aggcaacagc ttcgaggcta aaacatttta tgaacggacg gtcacccata
     4321 cctataaccc cgttttagaa atctatgcgc gtctcaatgc catccgccag aataaagaag
     4381 gcggcgaaga ctatatcgca aaaaatatcg aagccctcat aaaaatggca cacaaagacc
     4441 gatatgcggc ctaccgcgat atcatcta
//
LOCUS       DASYSV010000392         4027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1740814, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000392 DASYSV010000000
VERSION     DASYSV010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740814"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VGQ51_12035"
     CDS             <1..445
                     /locus_tag="VGQ51_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9037350.1"
                     /translation="RDRVKESFYHMITQGDYAARISGEDKEFLDRIIVVVEKNLEDEG
                     FCIKKLSQEVGMSHSTIYQRLKELSGLSVNGFVRWIRLKKAAELFITTNLNVNEVALQ
                     VGFGDGKYFREQFNKLYGVNPSEYIKKYRKMFSGKYHLDRDALKL"
     gene            558..3569
                     /locus_tag="VGQ51_12040"
     CDS             558..3569
                     /locus_tag="VGQ51_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9037351.1"
                     /translation="MKNCLPSEGTFLLLARFLTVLLCLSFSSDLWAQDISVKGRVTSG
                     DSVLSGVTVSVKGTGKATQTNAEGNYSLEAPGGGTLVFSRVGFAGQEIGIRGRAVVDV
                     QMVIANQQLSDVVVVGYGTQKRVDVTGAVSSVPKDRLSQLPVTNVMQAVEGSVPGVTV
                     TNTSSVPGAQPTILVRGQNSITANSGPYIVVDGFPMSKTNGTLNDINPNDIASIEILK
                     DASATAIYGTNGSNGVILVTTKRGATAKPQLRFSAYTGVDKVSHELTPRDGPSYVQKY
                     KDFMTETNQVQTGPVPNATELPNYQSGKTIDWMKQVFQQGVTNDNTLSLSGGTKDARY
                     FISGEYMKQKGAVKGYQYHRASVRANLDINVADFLTVGTSAYYTYNNYDGGQANFLFA
                     GAMSPYGNEYNADGSYVIYPMYPELLYTNPLLGLNTSNVNHNANLYGNGYAEVKFGGV
                     LKGLKFRLNAGTTYTPAATYTYTGRNANNLLGNATTLNSETNSYILENLLTYSRDFGE
                     HHIDFTGLYSAEQRKYFIDAYSATGFVNDLLTYSNIGAASTLTAASTGSGLPNGSYQS
                     RYALNSQMARINYSYGDRYLLTLTARRDGSSVFGANTSKFDVFPSAAIGWNVSKEAFM
                     NNVRFVDNLKLRGSYGKTGNEAINPYQTITTDNPVQNTFNGVTTIGVIAGTLGNAGLH
                     WEATTQTDVGIDFAVLHSRVTGTIDVYHANTDGLILSRQIPNITGYASVLYNVGKTSN
                     DGLELTLTTRNIDLKDFHWETMLNYSANRNKILDLYGDHKSDTGNVWFIGHPIGVVYD
                     YVRQGVWQVGEDPSKQDPSAKPGDLKFADLTHDGKVTASDKTILGQTRPKWTGGMTNT
                     FSYKNFRLSVFIQTVQGVTKNNADLNYVDETGRRNTPAAIGYWTPTNKSNYWPGLSYF
                     NSRGYGYGSNASFTRIKDLTLSYSFPEKMLERAKLGGLTLYASCRNVYTWSKWIGWDP
                     ENNYSMRGTGTWTTNYPEVRSIVFGLNVSLR"
     gene            3590..>4027
                     /locus_tag="VGQ51_12045"
     CDS             3590..>4027
                     /locus_tag="VGQ51_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9037352.1"
                     /translation="MKKNIIILIVSVGLLSSLAGCKKSFVAEKPFSSYSPQTLTDSLG
                     FEAAVNGLYYQLSLYYTYSDHQGWPSLWQVGTDVAFATQPEGIEVPYYNYATLISTDA
                     GAAYEWSWAYKLVNDANNIIANVENPSLKGISAADISDVDGEAR"
BASE COUNT          984 a          998 c         1141 g          904 t
ORIGIN      
        1 gagggacagg gtgaaggaat ctttttatca tatgatcacg cagggagact acgctgcccg
       61 gatctcgggg gaggacaagg aattcctgga ccggatcatc gttgtggtgg agaaaaatct
      121 cgaggatgaa ggtttttgta taaaaaagct gtcgcaggaa gtgggcatga gccattcgac
      181 catttatcag cgtttgaaag agctgtcggg gctgtccgtc aatgggtttg tccggtggat
      241 ccggctgaaa aaggcggccg agctgttcat aacgactaat ttgaatgtca atgaggtggc
      301 gttgcaggta gggtttggcg atggcaagta tttccgggag cagttcaata agttatatgg
      361 ggtcaaccct tccgagtata ttaaaaaata caggaagatg ttttccggaa agtatcatct
      421 cgacagagat gcgctgaaat tgtgagaagg agggcggccc caagctgaaa ttgtgagatt
      481 accccccttt ttctgagatt acccccctga ccacttgggt tcgtcttggc tatattgcca
      541 gtctaaaaca actggttatg aaaaattgct tgccaagcga aggcactttt ttgctactcg
      601 cccggttctt aaccgtgctg ctttgcctct ccttttcatc ggatctgtgg gcgcaggaca
      661 tcagcgtcaa aggccgggtg acctcgggtg attcggtcct cagcggtgtc acggtgtcgg
      721 tgaagggtac gggcaaggct acccaaacca acgcggaggg gaactattct ttggaggcgc
      781 cggggggcgg caccctggtc tttagccgtg tgggttttgc cggtcaggag atcggcatac
      841 gtgggagagc ggtggtggac gtgcagatgg tgatcgccaa ccagcaattg tcggatgtag
      901 tggtagtcgg ttatggcacc cagaaaaggg tggatgtgac gggggctgtc tcctcggtac
      961 ccaaggaccg gttgtcccag ctgccggtga cgaacgtcat gcaggccgtc gagggttcgg
     1021 tgccgggagt aaccgtgacc aacacttcat ccgtccccgg tgcgcagccc accatcctgg
     1081 tgcgcggaca aaactccatc acggccaact cgggccccta tatcgtagtc gatggattcc
     1141 ccatgtcgaa gaccaatgga accctgaatg acatcaatcc caacgatatt gcctctatcg
     1201 agatattgaa ggatgcctcg gcgaccgcca tttacgggac caacggttcc aatggggtca
     1261 ttttggttac gaccaaacgg ggtgcgactg ccaaacctca attacgattt agcgcctata
     1321 cgggtgtaga caaggtatcg catgagctga cgccgcggga tggaccttcc tatgtacaga
     1381 agtacaaaga tttcatgacc gagacgaacc aggtgcagac cggccccgta ccgaatgcca
     1441 ccgagctgcc caattatcaa tcagggaaga ccatcgactg gatgaagcag gtgttccaac
     1501 agggggtcac caacgacaat accctttcgc tttcgggcgg tacgaaggat gcccggtatt
     1561 ttatctccgg ggagtatatg aagcagaagg gcgccgtcaa aggctatcag tatcaccggg
     1621 ccagtgtccg cgccaacctg gacatcaacg tcgccgattt tctgacggta gggacctcgg
     1681 cctattatac ctataataat tatgatggcg ggcaggcgaa ctttttgttt gccggggcga
     1741 tgagtcctta tggcaacgag tacaatgcgg acggcagcta tgtgatctac ccgatgtacc
     1801 cggaactgtt gtataccaac ccactgcttg gtctgaatac gtcaaacgta aatcacaatg
     1861 ctaacttata cggtaacggg tatgcagaag tgaagttcgg aggagtactg aaggggttga
     1921 aattccggct caatgccggg acgacgtata caccggctgc gacgtatact tatacgggcc
     1981 ggaatgcgaa taacctgctt gggaatgcga cgacgcttaa ctcggaaacg aactcgtata
     2041 ttctcgagaa cctgctgacc tattccaggg atttcgggga gcatcatata gactttaccg
     2101 gtctgtatag cgccgagcag cgaaaatatt tcatagatgc gtatagcgcc acggggtttg
     2161 tgaacgatct gctgacctat agcaatatcg gcgccgcatc cacgctgacg gcggccagca
     2221 ccggctccgg tctgcccaac ggctcgtatc aatccaggta cgcgctcaac tcacagatgg
     2281 cgaggatcaa ctattcgtat ggcgatcgtt atctcctgac gttgaccgcg cggagggacg
     2341 gatcatcggt attcggcgcc aatacgagca aattcgatgt gttcccttcg gcggcgatcg
     2401 gttggaatgt ctccaaagag gcgttcatga acaatgtccg tttcgtggac aatctgaaac
     2461 tgcgcggatc gtacggaaag acgggtaatg aagccatcaa cccttatcag acgatcacca
     2521 cggacaaccc cgtgcagaac accttcaacg gagtgacgac gataggagtg atcgcgggaa
     2581 cccttgggaa tgccggtctg cattgggaag ccacgaccca gaccgatgtc ggcatcgatt
     2641 ttgccgtgct gcatagccgt gtaactggta ccatcgacgt ctatcatgcg aataccgacg
     2701 gattgatcct cagccggcag atacccaata taaccggtta cgcgagcgta ttgtacaatg
     2761 tcggaaagac cagcaacgac ggcctggagc ttaccctgac gacgaggaat atcgatttga
     2821 aagatttcca ttgggaaacg atgctcaatt attccgccaa ccgaaataag atactcgatc
     2881 tttacggcga ccataagagc gataccggca acgtatggtt tatcggtcat cccatcggcg
     2941 tggtctatga ctatgtccgg cagggtgtct ggcaggtagg ggaagaccct tccaaacagg
     3001 acccgagtgc gaaaccgggg gatctgaaat tcgcggatct cacgcatgat ggcaaggtta
     3061 cggcttcgga caagacgatc ctggggcaga cgcgccctaa atggaccgga ggcatgacga
     3121 ataccttttc gtataagaat ttccggttga gcgtgttcat tcagaccgtg cagggcgtca
     3181 cgaagaacaa tgccgacctc aactatgtcg atgagaccgg cagacggaat actccggcag
     3241 ccatcggtta ctggacaccc acgaataaga gcaattactg gccgggcctt tcttatttca
     3301 actcccgcgg ttacggttat ggctcgaatg ccagctttac gcggatcaag gatctcaccc
     3361 tcagctatag ctttcccgag aagatgctgg agcgggcaaa gctgggcggt ctgacgcttt
     3421 atgccagctg tcgcaacgtg tatacctggt cgaagtggat cggctgggat ccggaaaaca
     3481 actactcgat gcggggaacc ggcacctgga ccactaacta tccggaagtt cgcagtatcg
     3541 tattcggtct caacgtatca cttcgttaaa atctgtcaaa accaagaaaa tgaaaaagaa
     3601 tatcatcata ttgatcgtgt cggtcggatt attatcgagc ctggccggct gtaagaagag
     3661 ctttgtggcg gagaagccgt tttcgtccta ttcgcctcaa acgctgaccg attcgctggg
     3721 tttcgaggcg gcggtcaacg ggttgtatta tcaactgagt ctttattata cttattcgga
     3781 tcatcagggt tggccgagcc tctggcaggt gggcaccgac gtggccttcg ccacacagcc
     3841 ggagggcatc gaagtgccat actataacta cgctaccctg atctccaccg atgcgggggc
     3901 cgcctatgaa tggagttggg cgtataagct ggtcaacgat gcgaacaaca tcatcgcgaa
     3961 tgtggagaac ccttctttga agggcatcag cgcagcagat atctcggatg tcgatggcga
     4021 ggcgcgt
//
LOCUS       DASYSV010000393         5506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_976934, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000393 DASYSV010000000
VERSION     DASYSV010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976934"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VGQ51_12050"
     CDS             complement(<1..1010)
                     /locus_tag="VGQ51_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9037353.1"
                     /translation="MDSTIIIGAGAAGLMAGRRLSAVGHQVTILEARPSAGGRIFTLP
                     SRESHGFSVAAEGGAEFIHGNLPISLGLAEEAGIFLQPVAAEMVGMRQGKEPDKEQRN
                     RQSKDWGLLMQRMAMQGQDLPLADFLAEWFPGDQYPGLRDSAGRMAEGYDLADLHRVS
                     THSLYREWSGESDEEEYRPEGGYGPMIGYLAEVCRRQGCELQFSTIVTAVRWQRGRVE
                     VTTTRGQVFIGTRLITTVSLGVLKAGSIRWSPGLPRQQEAISKLGFGSVIKVLLEFNQ
                     PFWRHRKKPGRTLFIVSDEPAPTWWTQTADECPLLTGWIAGGPMREFRKIGPDEKLEV
                     CI"
     gene            1219..1500
                     /locus_tag="VGQ51_12055"
     CDS             1219..1500
                     /locus_tag="VGQ51_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037354.1"
                     /translation="MQIQFIRNIQFTKLVKADGRLREFNFRKSNGLQEGLFTVDVSDD
                     RGNRIVLRMQREDGVWKIVKQQLPAWIWENEATFHTLIEEELGAAGIVK"
     gene            complement(1452..3419)
                     /locus_tag="VGQ51_12060"
     CDS             complement(1452..3419)
                     /locus_tag="VGQ51_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HEV9037355.1"
                     /translation="MKSFCVVAWMVVFLAGATERSPAQSGEVARLHSSLRGLGEGNLT
                     DSAYVDTLNRLVHLFYGINSDSAFHYAHMALDYAARIHYRKGEAESWRMLGNTFEMVG
                     DYLNMLSCYQHSLDIAEEIGNPNLIGKANVNIALFYKQEGEFDQAQELMQKVQDLYKA
                     NGDSVQSAYISSYLADLAFRQHQYELALQYAGRALEAARSIRDAPTIATFNNDFGRIL
                     AATGNYPAALDHHLQSLIYYRGVDDWLGTTSTNSLLAQDYLLLKDYPQALKYANEALA
                     EARAIRRKLEAQGSAKVLADIYEAKGDYRNALKYYQLYKEYSDSLFNDQSSKQIYARA
                     AQYDYERQEARLREAQALKDAGYERALRRDDLKISITAFVIVLLSLLAFILWRERAVN
                     RRVNRLLHEKNDKIEEQKEILEQQAVQLLLSNQQKDKLFSIIAHDLRGPLNSLKGLMD
                     FRKEKKLTDVEINQMMRELRSHVDSSSEMVGNLLYWASSQLNGMKVIPVLLPMDELVE
                     EVLHLYGPQAIGKKVLLSNRVPPSVVGYGDKDMIRAVIRNLVSNAIKFCHSGGVVLVD
                     GCKRGGEIEICITDTGTGMSEDALDRIRRKESFSNYGTASEKGTGLGILLCHEFAEAN
                     KGRFYAESEWGKGSRCYFTIPAAPSSSSISV"
     gene            3820..4689
                     /locus_tag="VGQ51_12065"
     CDS             3820..4689
                     /locus_tag="VGQ51_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV9037356.1"
                     /translation="MKILSRGTQIKLSGSPEQVETAKEKIDLLVQYLERNGHVSENYF
                     EQILGGDDAETVDHFVDRHPNDILVFGPNGKTVRARTVNQKRMVVAAEKNDIVFAIGP
                     AGTGKTYTAVALAVRALKNKMVKKIILTRPAVEAGESLGFLPGDLKEKIDPYLRPLYD
                     ALDDMIPADKLGYYMSTRTIEIAPLAYMRGRTLDNAFIILDEAQNATDLQLKMFLTRI
                     GANAKAIITGDLTQIDLPKNQKSGLAKALYILKNIDGIAHIELDEEDVVRHRLVKAII
                     KAYERDKQQDEIK"
     gene            4713..5111
                     /locus_tag="VGQ51_12070"
     CDS             4713..5111
                     /locus_tag="VGQ51_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037357.1"
                     /translation="MVKKFIPLFLLLVACNDSHSFKKAEDAEDAGREFIRASLDGNYE
                     KASFYLYADSTNKMMLAKWKKDYDHLDPEDQQKYKEASIMVINISPLNDSVTDFTYLN
                     SFKKDTTTIRIVKQKGDWFVDLEEILYHHR"
     gene            complement(5193..>5506)
                     /gene="crcB"
                     /locus_tag="VGQ51_12075"
     CDS             complement(5193..>5506)
                     /gene="crcB"
                     /locus_tag="VGQ51_12075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV9037358.1"
                     /translation="LMTICVSKHMTIAFPMGTFLVNISGCFVIGLLFGLATRYAWLDA
                     DWRLFLITGICGGYTTFSSFSYESIQLIRQGDYAYFFTYVLGSVILGLLATVGGLALV
                     K"
BASE COUNT         1392 a         1570 c         1320 g         1224 t
ORIGIN      
        1 tcaatacaga cctccagctt ttcatccgga cctatcttcc tgaactcccg catcgggccg
       61 ccggcgatcc agccggtgag taacggacat tcatccgcgg tttgcgtcca ccatgtcggc
      121 gccggctcgt cactgactat aaaaagggtt cgccccggtt ttttccggtg acgccaaaaa
      181 ggttgattga attccaacaa aactttgatc accgatccga agcccagctt tgaaatggct
      241 tcctgttgcc gcggcagacc gggagaccac ctgatagacc cggccttcaa tacacccagg
      301 gataccgtgg ttatcagccg ggtgccaata aacacctgtc cgcgtgtcgt ggtaacctct
      361 accctgcccc gctgccagcg aacagcggtg actatcgtcg aaaattggag ctcgcaaccc
      421 tgtctgcggc aaacctcggc cagatacccg atcataggcc cgtatccacc ctctggccgg
      481 tattcctctt catccgactc accggaccat tcccgataca gcgaatgcgt actcacccgg
      541 tgcagatcgg ccagatcata gccttcggcc atgcgccctg ccgaatcccg gagcccggga
      601 tattggtcgc ccggaaacca ttctgccaaa aaatcggcca gtggcaggtc ctgcccctgc
      661 atggccatcc tttgcatgag cagcccccag tccttcgatt gccggttacg ctgttcttta
      721 tcgggctctt ttccttgtcg catcccaacc atctcagcag caacgggctg taagaaaatt
      781 cccgcctcct ctgccagccc gaggctgata ggcaggttac catgaatgaa ctcggcaccg
      841 ccttcggcag caacggaaaa tccatggctt tcacgggatg gcagcgtgaa tatcctgccg
      901 cccgctgaag gacgagcttc cagtatcgta acctgatgtc ccaccgcgga aagacgccga
      961 cccgccatga gcccggcagc tccggcccct atgatgattg tcgaatccat tgccggcaaa
     1021 aatggggaca aaaaattaca aattcatcct cccggggtgg gtttttttgc tatttttgta
     1081 agtgcctgtt gtaatttacc taccaagccc tgcgtaaaaa atcccgatcc tttaactgtc
     1141 taaaaattca caatccttta agaagcctga tccaaactca gcacaagaat tgttaaatat
     1201 tatttcaaag ggaaccttat gcaaatacaa ttcatccgga acatccaatt caccaaattg
     1261 gtaaaagccg acggccgatt gagagaattc aacttccgca agtcgaatgg actccaggaa
     1321 ggacttttca ctgtagatgt gagcgacgac cgcggtaacc ggatcgtttt gagaatgcaa
     1381 cgtgaagacg gcgtctggaa gatcgtcaaa caacagttac ctgcctggat atgggaaaat
     1441 gaagctacat ttcacacgct gatcgaagaa gagcttggcg ccgcagggat cgtaaagtaa
     1501 cagcggcttc cttttcccca ttcgctctcc gcatagaacc gtcctttgtt cgcctcggca
     1561 aactcatggc atagcaggat acccagcccg gtgcctttct cactggccgt tccatagttg
     1621 gaaaagcttt cttttcgcct gatccgatcg agagcgtcct cgctcatccc ggtgcctgtg
     1681 tcggtgatac agatctctat ttccccgcca cgtttgcagc catcgacaag caccacgccc
     1741 ccggaatgac agaacttaat ggcattgctg accaggttgc ggatgaccgc ccgtatcata
     1801 tccttgtcgc catagccgac gacagatggg ggcacccggt tggaaagcag tacttttttc
     1861 ccgatggcct ggggaccata caggtgcagc acttcctcca ccaattcgtc catcggcagc
     1921 aggacgggaa taactttcat gccgttgagc tggctgctgg cccagtacag gagattacct
     1981 accatttccg aagaagagtc gacatggctg cggagctcgc gcatcatctg gttgatctcc
     2041 acgtcggtca gctttttttc tttgcggaaa tccatcaacc ccttcaggga attgagaggc
     2101 cccctaaggt catgcgcaat gatagagaac agcttgtcct tttgctggtt gctcagcagc
     2161 agctgaacag cctgctgttc cagtatttct ttctgctctt cgatcttgtc gttcttttca
     2221 tgcaacagcc tgttgacccg ccggttcacc gcccgctcac gccagaggat gaaggcgagc
     2281 aggctcaaca gcacgatcac aaacgccgtg atggatattt tcaggtcatc ccggcgcagg
     2341 gcgcgctcat acccggcatc cttgagagcc tgagcctctc tcagccgggc ctcctgtctt
     2401 tcatagtcat attgcgccgc gcgtgcatat atctgtttgc tggattgatc attgaacaga
     2461 ctatcggagt attccttata gagttgataa tatttcagcg cattccggta atcccccttc
     2521 gcctcgtaga tgtccgccag cactttggca gacccctgag cctccagctt acgccggatg
     2581 gcccttgctt ccgccagcgc ttcattcgcg tatttcaacg cctggggata gtcttttaac
     2641 aggagataat cctgggcaag caggctattg gtagaggtgg tgcccaacca gtcgtccact
     2701 ccgcggtaat agatcagcga ttgaagatga tgatccaatg cagcgggata gttgccggta
     2761 gccgcgagta tcctcccgaa atcgttgttg aacgtggcga tggtcggtgc atcccggatg
     2821 ctacgggcgg cctccagcgc ccgtccggca tattgcagcg ccagttcgta ttggtgctgg
     2881 cgaaaggcaa ggtcggccag gtaagaagaa atataggccg attggacact gtccccgttg
     2941 gccttataga ggtcctggac cttctgcatc agctcctggg cctgatcgaa ctccccttcc
     3001 tgtttgtaga acaaggcgat attgacattg gccttgccga taagattggg attgccgatc
     3061 tcttcggcga tatccagcga gtgctggtaa caggaaagca tgttcaggta atcgcccacc
     3121 atctcgaagg tattgcccaa catccgccag ctttccgcct cccccttgcg atagtggatc
     3181 ctggcggcat aatccagggc catatgcgca tagtggaacg cactgtccga attgatgccg
     3241 taaaaaagat ggaccaggcg gtttagagta tccacgtagg cggaatccgt cagattgccc
     3301 tcccccagcc ctctcagcga ggaatgcaga cgggccacct cacccgactg agccggggat
     3361 ctctccgtcg ctcccgccag aaagaccacc atccatgcca caacgcagaa agatttcatt
     3421 caggctacag tttacaataa attgtgactt cttgaatacg aacaaactcg taagaacgtt
     3481 gttaaatatt ataattcagc tcggtggtag aggaacgcag ttatacagta ggaaaggcag
     3541 ccgggcaaca aacataggaa ttatattcac atgctgttaa taagaattgt agaaaaaaga
     3601 gggctctccg gtctatattt gattagatac ccgccggggg cccggtagaa aagtaatccc
     3661 caaccggccg gaggccggtt aaaagtaatc cgttttctca acgctaaaaa agcacagaat
     3721 ttgactgaaa ctattattaa cctggaaacc gttaacccca ttgaattttt cggtgtaaac
     3781 aacggtaaat tagacattct gaagaaaaag tttcccttat tgaagatcct gtcacgcggt
     3841 acccagataa aattgagcgg atcacccgaa caagtggaga cagcaaagga aaaaatcgat
     3901 ttgttggtgc aatacctgga acgaaacggt catgtcagcg agaactattt cgagcagata
     3961 ctgggcggtg atgacgccga gacggtagac catttcgtcg accgccatcc caacgacatc
     4021 ctggtattcg gccccaacgg aaagactgtc agggcccgga ccgtcaacca gaaacgaatg
     4081 gtcgtcgccg ccgaaaagaa cgatatcgtg ttcgccatcg gacctgccgg tacgggtaag
     4141 acctataccg ccgtcgccct ggccgtccgg gcattgaaaa acaagatggt caaaaagatc
     4201 atcctgaccc gtccggcagt ggaagcaggc gaaagtctcg gttttctccc cggcgatctc
     4261 aaggaaaaga tagatcccta tcttcgcccc ctctatgacg cattggatga catgatcccc
     4321 gccgataagc taggctatta tatgagcacc cgcaccatcg aaatcgcccc cctggcttac
     4381 atgcgcggcc ggacgctcga caatgctttc atcatcctcg acgaagcaca gaatgccacg
     4441 gatctgcagt taaagatgtt cctcacccgt ataggggcca acgccaaggc catcatcacc
     4501 ggcgatctta cccagatcga tctgcccaag aatcagaaaa gcggtctggc aaaggcactc
     4561 tatatcctaa agaacatcga cggcatcgcc catatcgagc tggacgagga agacgtcgtc
     4621 cgtcaccgcc tcgtcaaggc catcatcaaa gcctacgaaa gagacaaaca acaagacgaa
     4681 attaaataac gaaagcccgc tcttcgagtt caatggtaaa gaaattcatc cccctgttcc
     4741 tcctcctggt cgcctgtaac gattcgcatt ctttcaaaaa ggccgaagac gcggaagatg
     4801 ccggtcgtga attcatccgt gcctcgctgg acggcaacta cgaaaaagcc agcttctatc
     4861 tgtacgccga ttcgacgaac aagatgatgc tggccaaatg gaaaaaggat tatgatcacc
     4921 tggacccgga agatcagcag aaatacaagg aggcttcgat catggtgatc aatatcagcc
     4981 ccctcaatga ttcggtcacc gatttcacct atctgaattc gtttaagaag gatactacca
     5041 ccattcgcat cgtcaaacaa aaaggcgact ggttcgtcga tctcgaagag atcctgtatc
     5101 accatcggta gatccggctc ctgcttttcc ggcggttgcg ctaccttgcc atccggcgca
     5161 ccgcgctcta tctccccgca cgacgtcccg tcctacttca ccagggccag accgcccacg
     5221 gtggccagca atcccagtat cacactgccc aggacatacg tgaagaaata cgcataatcg
     5281 ccctgccgga tcaattgaat actttcatag gaaaaactgg aaaacgtggt ataacctccg
     5341 catattcccg tgatgaggaa caaacgccaa tccgcatcca gccaggcata acgcgtagca
     5401 agaccaaata aaagaccgat gacgaaacaa cccgagatat tgacgagaaa agtacccatc
     5461 gggaaagcga tggtcatgtg cttgctgacg cagatcgtca tcagat
//
LOCUS       DASYSV010000394         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1231610, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000394 DASYSV010000000
VERSION     DASYSV010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231610"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VGQ51_12080"
     CDS             <1..576
                     /locus_tag="VGQ51_12080"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV9037359.1"
                     /translation="KLMPGVTVTASILEIGGERDPDRGLQKAIDAKDSIGGIIECRVN
                     GLPVGLGEPFFDSVESLLAHMVFAIPAVRGVEFGTGFAAARMFGSEHNDAIEDMEGRT
                     RTNHAGGVVGGITNGNELVFRIAIKPTSSTPKEQQTLNWETEKVEAFSVKGRHDLCVA
                     LRAPVIVEAVTAIVLADLLLLEQHSPRVLGK"
     gene            685..1275
                     /locus_tag="VGQ51_12085"
     CDS             685..1275
                     /locus_tag="VGQ51_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEV9037360.1"
                     /translation="MEPSVQDLILQFNRGSAQAYTIIYNLCSPSIYYFAKRFVGDRQA
                     AEEITADSFIKLYRLHDNFDSLPNIRAFLRITTRNACLNYLRSLRSADAQKQELLYVL
                     SQEGGAESPVDQYQSDLLQRINEEVEKLPKKCRQVFKMAYMEGRGNEEIARILNINYQ
                     SVKNQKTRALKILRLALLNKGVLAFFLIRIYRVFLR"
     gene            1309..>2383
                     /locus_tag="VGQ51_12090"
     CDS             1309..>2383
                     /locus_tag="VGQ51_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9037361.1"
                     /translation="MYKYLLVVLICFALPPAAMAQAPAGVIDGAVRDSVSGNMLEEAT
                     VSLLVSRRESTDRIFAVRRNGSRGFAFRGLAAGDYMIVSTYLGYRPDTMWIRLAEKQT
                     AGARVLLRMQRSDNPMMQVVVTAHIPPVIVRHDTIAFNTAAYPTQPHATLEDLLRKLP
                     GIDVDQNGNVTMQGKKVDKVYLDGKEFFLGDPRTATQNLPADIIDQVQAFDSKTDRER
                     LTGIKDMTNTTSLNIKLKKNRRKGIFGKLYAGAGTGAAKPIGSGSSIGSGSANGSGSP
                     VGAGSSGADPSYSVGGTVMTLGKTTLMARGYVNNMNNQFTGTENRNGPGAAGVQTLNK
                     LDLNFRREIGKVSITLNGGTDGHH"
BASE COUNT          545 a          554 c          747 g          537 t
ORIGIN      
        1 aaattgatgc cgggggtgac ggtgaccgcg agtatcctgg agatcggagg agagcgggat
       61 cccgatcgcg gtcttcagaa agccatcgat gcgaaagatt cgatcggcgg tatcatcgaa
      121 tgccgggtaa atgggttacc ggtgggtttg ggagagcctt ttttcgactc ggtggagtca
      181 ttgctggcgc atatggtctt tgcgataccg gcggttcgcg gcgtagagtt cgggaccggc
      241 tttgcggcgg ccaggatgtt tggaagcgag cataatgatg caatcgagga tatggagggg
      301 aggacgcgga ccaatcatgc gggtggtgtg gtaggcggga tcaccaatgg gaatgagctg
      361 gttttccgta tcgctatcaa gccaacgtcg tccacgccga aggaacagca gacgctgaac
      421 tgggagacgg agaaggtaga agctttttcg gtgaaggggc ggcatgattt atgcgtggcg
      481 ctgcgcgctc cggtgatcgt ggaagccgtg acggctatcg tgctggcaga tttgctattg
      541 ttggaacagc attcgccgag ggtattgggg aaataagtat atttggcgaa tgcaggtatc
      601 tgtggaaagc cttaggcgga cgaaaaatcc gctggccgaa ggccaatgac ctgtagccta
      661 taaaagcgtt aaggagctga actgatggag ccatctgtac aagacctcat tcttcaattc
      721 aaccggggaa gtgcccaggc ttatactatt atctataatc tttgctctcc gtccatttat
      781 tattttgcca aacgtttcgt gggtgaccgg caggccgcgg aggagatcac cgcggatagt
      841 ttcatcaagc tttatcgtct tcatgacaat ttcgacagtc tgccgaatat ccgggccttt
      901 ctccggataa ccacgcgcaa tgcctgtctc aattatctgc ggagtcttcg ttcggcggat
      961 gcacagaaac aggagctgct ctatgtgctg tcgcaggaag ggggggcgga gtctcccgtc
     1021 gatcaatacc agtcggactt gctgcaacgt atcaatgaag aggtggagaa actgccgaaa
     1081 aaatgccggc aggtgttcaa gatggcgtat atggaggggc gggggaatga ggagatcgcc
     1141 cggatactga atatcaatta tcaatcggtc aagaatcaaa agaccagggc attgaagatc
     1201 ctgcgtctgg cgttgctgaa taaaggtgtg ctcgcgtttt tcctcatcag gatctatcgt
     1261 gtttttctcc ggtgaagctc cggtgaagga aaatttcact gtctcgttat gtacaaatac
     1321 ttgttggtcg tccttatttg ttttgcattg ccccccgcag ccatggcgca ggctccggcc
     1381 ggcgtgatcg acggcgccgt tcgcgatagc gtctcgggaa acatgctcga ggaagccacc
     1441 gtcagtttgc tggtgtccag gcgggaaagc acggaccgca tttttgccgt taggcgcaac
     1501 ggctccaggg gatttgcctt tcgggggctg gccgcgggcg attatatgat cgtgtccact
     1561 tatctcggtt accgtcccga tacgatgtgg atcaggctgg cggaaaagca aacagcagga
     1621 gcccgggtct tgctgcgaat gcagcgttcg gacaacccga tgatgcaggt ggtggtgacg
     1681 gcgcatatcc ctcctgtgat cgtgaggcat gataccattg cttttaatac ggccgcatat
     1741 ccaacccaac cgcatgctac actggaggac ctgctgcgca agttgcctgg tatcgatgtc
     1801 gatcagaacg gtaatgtgac gatgcagggt aagaaggtgg ataaagtgta tctcgacggc
     1861 aaggaatttt ttctcggtga cccacggacg gcgacgcaga atctgccggc tgatatcatc
     1921 gaccaggtgc aggccttcga cagcaagacc gacagggagc gtcttacggg gatcaaggac
     1981 atgacgaata cgacgagcct caatatcaag ctgaagaaga accggcggaa gggaattttc
     2041 gggaagctgt atgcaggtgc agggacgggg gcggccaagc caattggatc cgggtcttcc
     2101 attggatcgg ggtctgccaa tggatccggc tctcccgttg gagcgggctc ctccggggcc
     2161 gatcccagct attcggtggg cggcactgtt atgacactgg gtaagaccac gttgatggcc
     2221 cggggttatg tcaataatat gaataatcaa ttcaccggga ccgagaacag gaacgggcca
     2281 ggtgcggccg gcgtacaaac gctgaataag ctggacctga acttccggcg ggagatcggg
     2341 aaggtcagta ttacccttaa tggcggtacc gacgggcacc ata
//
LOCUS       DASYSV010000395         4998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_204131, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000395 DASYSV010000000
VERSION     DASYSV010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204131"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGQ51_12095"
     CDS             complement(<1..351)
                     /locus_tag="VGQ51_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEV9037362.1"
                     /translation="MFSSLCWGFSSCVNTKKAIYFREQGEGTIRSSSVVPETYIQSND
                     ILSIQVGSLNQDASSAFNVANKADVSATTLSGSRNETAGYLVSPEGYITFPILGEIPA
                     AGLTKTQLTDTLTRM"
     gene            complement(687..1943)
                     /locus_tag="VGQ51_12100"
     CDS             complement(687..1943)
                     /locus_tag="VGQ51_12100"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEV9037363.1"
                     /translation="MHLFFLLLIRAFLLSAPPSLNVHDFGAKGDGVTDDTQAINQALQ
                     AAHDQQANVYFPSGTYLCNTLGSDGNILNLHADGLQGITIYGDGSTSVLTTSLNQGSV
                     LLFVLAFSPCSNLTVKNLSFENTHGMITQQTRGLFFLGKVNQFITNLTVSGCYFNGFS
                     TALGGQGINTWLVNSNYFGSPKGHDNAKDDTDPAVFLWCYDNQNGFCKDVTITNNTAD
                     GYTGTGPLSALVTHRPMDGFVFGAAYGMMIANNTTRNFCEEHYNLQPKATNPNDTTTA
                     TITNNYLDCSLPPGCSNTDGSPHHSNYGIRDDISYAVITNNTMVNYTYGIIIRAIEYP
                     NTQLNHYIITGNQLHAATDTVAYNVTAAILIQGNANPVSDIRMTDNDIYTNIPDLPNF
                     PRSIRVERTQNLLRTLITNNNLHMIP"
     gene            2034..4349
                     /locus_tag="VGQ51_12105"
     CDS             2034..4349
                     /locus_tag="VGQ51_12105"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV9037364.1"
                     /translation="MHKPLLLCFALSALTARPQSPATARPQTPAADDPLQYVKPIVGT
                     QRMGHTYPGATVPFGMVQLSPDTDTIPYELNGKYNPDVYKYCAGYQYDDKTIVGFSHT
                     HFSGTGHSDLGDFLVMPTVGELKLNPGVATQPHSGYRSAFRHETETAEADYYKVRLDD
                     YNIVAELTATTRVGFHQYTFPASDQAHIVLDCISGIYNYPDKNVWTYIRVVNDTLVTG
                     YRQTSGWARTRTVYFAMTFSKPFERYGSRKYDKREVYRGFWGHFDQNHDFPDLAGRQL
                     RLYFNFKTTEGEKIRIKFALSPVSMQGALANLRTETPGWDFDAVRRKGQEAWKTELSK
                     VVIDAPTAEKENIYTSLYHAYINPTVYMDADGSYRGLDQENHVAGGFVNYTTFSLWDT
                     HRALHPLFSILQPRRNFDMIRSMLAHYDQSPEHMLPIWSNSANENWCMSGYHAVSVIC
                     DAITKGSTPFDPNKALDACIATSNRRSFEGIGEYIDRGYISSEADGSSVSTTLEYAYD
                     DWCIAQAAKKLGRMEDFSVYSKRAENYKNVFDPGIGYMRPRLADGSFRKDFDVLSTNG
                     QGFIEGNSWNYTFYVPHDPAALIAMLGGDKRFIRRLDSLFTMELPDKFFAETEDITRD
                     GIIGGYVHGNEPSHHVAYLYNWTDQPWKTQERVRMILKKQYGPTPDGLGGNDDCGQMS
                     AWYLFTALGFYPVAPGSDRYSLGSPAVNAAVLHLENGRTFSIEAVDQSDKNVYVRKIT
                     LNGQALNRLWITHQEIMDGGKLVFYMSEKHH"
     gene            complement(4632..>4998)
                     /locus_tag="VGQ51_12110"
     CDS             complement(4632..>4998)
                     /locus_tag="VGQ51_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037365.1"
                     /translation="TFNAAQVAGVYSHSLGFMAVLGYTISSNDTTGFQIQFSYVPPVG
                     ITFSSDTTATGLTYFVPGKRYDAFLGQGVVIVNLSVADTVNHKLAGTFSGTVYNDANP
                     NDSAVITNGKFISTYSVQP"
BASE COUNT         1188 a         1272 c         1357 g         1181 t
ORIGIN      
        1 catccgggta agggtatcgg tcagctgcgt cttcgtcaga ccggcagcag ggatctcgcc
       61 caaaatgggg aaggtgatgt aaccttccgg gcttaccagg tacccggccg tctcgtttcg
      121 gctacccgac aacgtggtag cgctgacatc cgccttgttg gcgacattga aggcggaaga
      181 ggcatcctgg ttcagactgc ccacctggat gctgaggata tcgttgctct ggatataggt
      241 ctcgggtacg acgctcgagg accggatggt cccttcgccc tgttccctga aataaatggc
      301 ttttttcgtg ttgacacagg aagaaaaccc ccagcataga ctactgaaca atattaattt
      361 acgcggccaa aggccaagac aaattgccat acttaacagg atgaattgac gaaatcaata
      421 gggcaggtgg cggctgcttt agtaggatgg aaatcgaggt gttcggagga cgtatggcat
      481 tcaatagcgg aatttcgcag taaggtcttt cattggataa gtatcctcag tccgaatagt
      541 atgccaagat gccgaaatgg ggaaaagatc gtctggatag gccccccgca tagcaaacgg
      601 ttaaaaaatt atcggtgaat ggaaaatatt ttcccattta tttgacgatc aggccagttg
      661 gggaaatttt tggacagcgc tgatactcat ggtatcatat gcagattgtt attcgtgatc
      721 agcgtccgca acaggttttg ggtccgctcc acgcgaatgg accttggaaa gttcggaaga
      781 tcgggaatat tcgtatagat atcattatcg gtcatccgga tatcgctgac cgggttcgcg
      841 ttgccctgga tcagtattgc tgccgtcacg ttataggcga cggtatccgt cgccgcatga
      901 agctggttgc cggtaatgat atagtgattc agctgtgtat tcggatattc tatcgcgcgg
      961 atgatgatgc cataggtgta attgaccatc gtgttgttgg taataacggc atagctgata
     1021 tcgtcccgta tcccgtagtt gctgtgatgc ggcgatccat cggtgttcga gcaacccggt
     1081 ggcagggaac aatcgagata attgttggtg atagtagcgg tggtcgtatc gttcgggttg
     1141 gtagccttcg gctgcagatt gtaatgctct tcgcaaaaat tccgcgtcgt gttgttcgcg
     1201 atcatcatgc cgtaggcggc gccgaaaacg aaaccatcca tcgggcggtg cgtcacaagc
     1261 gcgctgaggg gtccggtacc cgtgtagccg tcggccgtgt tgttggtaat ggttacatct
     1321 ttacaaaacc cgttctgatt gtcataacac caaaggaaca cggccgggtc ggtgtcatct
     1381 ttggcgttgt catgtccctt cggagagccg aaataattgc tgttgaccaa ccaggtattg
     1441 atgccctgtc ccccgagcgc cgtactgaat ccgttgaaat aacagccgct gacggtgagg
     1501 ttggtaataa actgattgac cttgcccagg aagaacaaac cacgcgtctg ctgggtgatc
     1561 atcccatgag tattctcgaa ggaaagattc tttaccgtca ggttggagca ggggctgaaa
     1621 gccagaacga agagaagtac cgatccctgg ttaagactgg tcgtgaggac gctggtgctt
     1681 ccgtcgccat agatcgtgat accctgaaga ccgtcggcat gcaggttcag gatgttccca
     1741 tcgctgccca gtgtattgca cagataagtc cccgagggaa aatagacatt cgcctgctgg
     1801 tcgtgggctg cctgtaaggc ctgattgata gcctgggtat catcggtgac accatctcct
     1861 ttggcgccga aatcatgaac gttcagcgaa ggaggggccg ataggaggaa agcgcggatt
     1921 aataacaaga agaacaggtg cataatactt acaataaaat atgatgccaa actgcgctgg
     1981 tcttatacct ctttaggcta catttacacc accaaaaatg cgcctaatct actatgcaca
     2041 agcctcttct gctctgtttc gccctttccg ccctgacagc gcggccccag tcgcccgcca
     2101 ccgcccggcc gcaaacgccc gctgccgatg acccgctgca atatgtgaaa cccatcgtcg
     2161 gcacgcaacg catggggcat acctatcccg gcgccacggt ccccttcggt atggtgcagc
     2221 tgagtcccga tacggacacg atcccctatg agctcaacgg taaatacaac ccggatgtct
     2281 acaagtattg tgcgggttat cagtacgacg ataagacgat cgtaggcttt agtcataccc
     2341 atttcagcgg cacggggcat tccgatctgg gcgacttcct ggtgatgcct acagtgggag
     2401 agctgaagct gaacccgggc gtcgcaaccc agccgcacag cggctaccgg agcgccttcc
     2461 gtcatgagac cgaaacggcg gaagccgact attacaaggt caggctggac gactataaca
     2521 tcgtagccga gctgaccgct acgacccgcg tcggtttcca ccagtatacc tttcccgcca
     2581 gcgatcaggc gcatatcgtc ctcgattgca tcagcggcat ctacaactat cccgataaga
     2641 atgtctggac ctatatccgg gtagtgaacg atacgctcgt caccggctac cgccagacca
     2701 gcggctgggc caggacccgt acggtctatt ttgctatgac gttctccaaa cctttcgaac
     2761 gatacggcag ccgcaaatat gacaagaggg aggtctaccg ggggttctgg gggcatttcg
     2821 accagaacca tgattttccg gatctggcgg gtcgtcagct gagactctat ttcaacttca
     2881 aaacgaccga aggcgaaaag atcaggataa aattcgcgct gtcccccgtc agcatgcagg
     2941 gggcgctggc caatctccgg accgagacgc cgggatggga tttcgacgcc gtccgtcgta
     3001 aggggcagga agcctggaaa acggaattga gcaaggtcgt catcgacgcc cctacagccg
     3061 aaaaagagaa catttatacc tcgttgtacc atgcctatat caatcctacc gtttacatgg
     3121 atgcggatgg ctcttaccgg gggctggatc aggagaacca tgtcgccggc gggttcgtca
     3181 attatacgac attctcgctg tgggatacgc accgggccct gcacccgctt ttcagcatcc
     3241 tgcagccgcg gcgaaatttc gatatgatcc gcagcatgct tgcgcattat gaccaaagtc
     3301 ccgaacatat gctacctatc tggtccaatt cagccaatga gaactggtgt atgtcgggtt
     3361 atcatgccgt ctcggtcatt tgcgatgcga ttactaaggg cagcacgccg ttcgatccca
     3421 ataaggcgct ggatgcctgt atcgctacct ccaaccggcg tagcttcgaa ggcatcggag
     3481 aatacatcga cagaggctat atctcctccg aagccgatgg cagctccgtc tctacgaccc
     3541 tggagtatgc ctatgacgac tggtgtatcg cccaggcggc gaaaaagctc ggacgcatgg
     3601 aggatttttc cgtctacagc aaacgcgcgg agaattataa gaacgtcttc gacccgggta
     3661 tcggctatat gcgtccccgg cttgcggacg gcagcttccg gaaggacttc gatgtactga
     3721 gcacaaatgg tcagggcttt atcgaaggta actcatggaa ttacaccttt tacgtgccac
     3781 atgaccccgc cgcgctgatc gctatgctcg gcggcgacaa acgtttcatt cggcgtctgg
     3841 actcgttatt cacgatggaa ttgccggata agttctttgc cgagaccgag gacatcaccc
     3901 gggacgggat catcggcggc tatgtgcatg gcaatgagcc atcacaccat gtcgcctatc
     3961 tctacaactg gacggatcag ccctggaaga ctcaggagcg ggtgcgcatg atcctgaaaa
     4021 aacaatatgg tcccacaccg gatgggttgg gtggtaacga cgattgcggt cagatgagcg
     4081 cctggtatct ttttacggcc ctgggtttct atcccgtggc ccccgggtct gaccggtatt
     4141 cactcggaag tccggccgtc aatgcggcag tcctgcatct ggaaaatggc aggacgtttt
     4201 cgatcgaggc ggtcgatcaa agtgataaga acgtgtatgt caggaagata acattaaatg
     4261 gacaggccct gaacaggctt tggatcaccc accaggagat catggatggg ggaaagctgg
     4321 tcttttatat gagtgaaaag catcattaga ccgacttgtc taccatgaat tttgtgtcct
     4381 ttgaggcttg cagatatcgg atttttttta tatattgtaa atggataatc gatcttttta
     4441 ctttataaat tccgtcatat gaaaagagct acccatcatg tgcatttcgg agaagcggct
     4501 accaccattg gtttagccat tgccttcctt ggtttggtga tgttcctttc ctacattttc
     4561 atcaacgcca actcctaaac attgcgatcg gtaaataact gaggccggtt cttacgagcc
     4621 ggccttttca gttacggttg taccgaataa gtgctgatga acttgccatt ggtgatgacc
     4681 gcgctgtcgt tcggattggc gtcattgtat accgtcccgc tgaaagtacc cgccagttta
     4741 tggttcaccg tatcggcgac gctgagattc acgatgacca ccccctggcc gagaaaggca
     4801 tcatatcttt tgccgggtac gaaataggtg agaccggttg ccgtcgtgtc ggaagaaaag
     4861 gtgatgccca cgggggggac atatgagaac tggatctgga aacccgtggt atcgttcgag
     4921 ctgatggtat aacccagcac ggccataaat ccgagcgaat gcgaatagac accggcgacc
     4981 tgcgcggcat tgaaagta
//
LOCUS       DASYSV010000396        18196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2124694, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000396 DASYSV010000000
VERSION     DASYSV010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 18196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18196
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124694"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VGQ51_12115"
     CDS             complement(<1..438)
                     /locus_tag="VGQ51_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEV9037366.1"
                     /translation="MNDKPQRSLALDVFRGMTVCFMIIVNTPGSDTFAFSPLKHAHWH
                     GFTPTDLVFPSFLFAVGNAMSFSMKKFATMNQGAVLWKIIRRTLLIFLLGYLLYWFPF
                     FRQQADGHWGLAPISHTRIMGVLQRIGLCYGIASLLIYYCKPRT"
     gene            546..5906
                     /locus_tag="VGQ51_12120"
     CDS             546..5906
                     /locus_tag="VGQ51_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV9037367.1"
                     /translation="MKTKTLHSAYRLLPLFGSLLLLVSSLSAQPVRQNRPGVVRLKNG
                     VINVRRNIADGSLRRDSLLTAHFKRHYYVLAQFDRVPDTNLRAEMAHTGLHLYDYVSD
                     KAWLVELGDSFSIAQLQRFAIGGLTALSPPSKISRRLQDHRDEDLHDPDKAIAVGYFG
                     TLSPDQVRQEITATGAIIVPSKLQPPRVLFVRIPNPAILQRISALPFVSYITTQPIKP
                     RTLNYNNRAAQGPDALAASRNLYGDGVVVGVGDDTDPYTHVDFTGREIDRFDGPPGSG
                     HGVHTSGIVGGGGIINPMWQGMAPHSIILSQFFSDILVNTPAYMTDYDMPLTSNSYTD
                     YDYGCQYDGLYDALANYTDAQLLAYPNLMHVFAAGNDGGYTCTPFPPQFATIKSGFQS
                     AKNAISVGNVDNTNPYHDNTYSINSGSSSGPTDDGRIKPDLVAGGSAITSTLPYNSYG
                     QEWGTSMACPTVAGTLALLVQRYRQQHGGADPSAALLKALICNTATDLGNPGPDYLYG
                     YGSLDGKAAADAMDAVQYSFGTVSDGGTANANLIIPPGLAQVRIMIYWADYPAAPYAA
                     TALVNNLDLTVTDPSAVLHHPLILNPDPAHVNDNAVEGVDNVNNIEQVVLNNPSGGTC
                     NIAIHGTSIPQGPQSYVLVYQFIQPSVVLLYPYGRETWIPGYYPEYIRWNAFDNSPNT
                     FTLDYSTDNGATWTTINNAVSAASRMYMWTAPNVATNQALVRVTRNGTSYSSVSTYPF
                     TILGQPSASVTNPCQGYVQLSWNTIPSATSYDIMQLVGDTMVKVASTTTTSYLLGHLN
                     RDSSYWLGVRAVNGTSPGRRSISVNITPSGGACNLSALDNDYSADSLIGLNSGRMYTS
                     TQLTSAVPIEVELKNLGTIPTGSAFTVSYSINGATPVTEASSAVIPPNNGAFDYTFST
                     TADLSAPGSYTVQVWVSYPGDPATGNDTVTAVIKQLTNDPVTLNPAMTEGFESAAAAS
                     YVSPTMGFTGLDRCDFMTSNGNGRVRTFVNTGMAHSGNRYAVLDQTHYATSLTSDSLI
                     TTFNLSAYSSTDQIWLDFWYRNQGSTANLGGNTVWIRGNDQSAWISAYLLDTTTANIG
                     IYQPSSHVNVTQLLNNASETIGSSFQVKFGEVGNTGGSDVITDGTLDNGYIFDDITLT
                     KGTNDIGVLRLVSPVATGSCSLPGATPIGIGVRNYSNTAAVNIKVSYAIDGDTVTETI
                     PSIGAGDSIVYTFSATKDMSAFRAWRIDAWVSYTGDTYSLNDTLAPGTIQTTPLISAF
                     PYLESFDTSNGYWYTGGINSSWQWGAPKKSVIDKAASGPDCWVTSLTGNYNNNERSYL
                     YSPCFDLSGLTSPVLSFSHIFQTEDDCDCDYHWVEYTTDDVNWSRLGAVGAGTNWYDN
                     TTRQAWQLSWSKWHVSSYDIPVNAPKVRFRIVMNSDPGTTYEGVGIDDVHIFDKASVY
                     NGADDSVTKSVSGNGWINFDIGGRRIAAIDPNGQDLGPTTVKVFFNHTGAVRHDTVQY
                     YLDRNLVIQPSNPPVANVGVRYYFLDSEAVALINASGCLSCNTIADAYQSGVAQFSSP
                     AAAEEDSTLQNDSTGEFRFHHPHRDVAIIPNDNGYYAEYQVGGFSEFWICDTIPPETA
                     SPTLTLLSFTATRAGNTALLQWSTANAFGIDHYTIEKGTDSIHFSPLDSLPAATDGRG
                     TDNYQYTDTHLDSGANYYRLRAVDAFGNFIGSPIRKVEGPDSVSVIIYPNPVHRTYVF
                     ISTTRSARLIRLIDVSGRTVLVRSVRGNLNTVPLSALAPGIYFVEVTTDTGSTVQKIL
                     IR"
     gene            6122..6844
                     /locus_tag="VGQ51_12125"
     CDS             6122..6844
                     /locus_tag="VGQ51_12125"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEV9037368.1"
                     /translation="MDHTQDIKQASAELLYLISGLSISEALALLVKRYPGKVTFSTSF
                     GIEDQVITHHILSAGLPVSIFTLDTGRLFAETYSVWSATNEKYGTRLKAYYPDRRLLE
                     SYLEEKGPNAFYESVANRKECCHIRKVEPLQRALAGQTVWITGLRAEQSPQRADHQII
                     EFDEGNKIIKYNPLLHWSSDEVRDFIDQNNIPYNPLHDKGFVSIGCAPCTRAIRPGED
                     FRAGRWWWEDNSKKECGLHVAS"
     gene            6898..7815
                     /gene="cysD"
                     /locus_tag="VGQ51_12130"
     CDS             6898..7815
                     /gene="cysD"
                     /locus_tag="VGQ51_12130"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEV9037369.1"
                     /translation="MSNDTKYQLDYLDQLESESIHILREVAGQFEHPALLFSGGKDSI
                     TMVHLALKAFRPGKFPFPLVHIDTGHNFPEALDFRDRLVSTIGEKLIVRKVEDTIRRR
                     HLTEPGGKFSSRNALQSYTLLDTIEEFGFDACIGGARRDEEKARAKERIFSVRDEFGG
                     WDPKRQRPELWNIYNGRIRKGENVRAFPISNWTELDIWNYIRRENISLPSIYFAHERE
                     VIDYEGQWVAVSPFIRLDSGDSVQRRTVRYRTVGDMTCTAAVESSASVIDDIIREITA
                     TRTSERGETRIDDKVSEAAMEDRKKNGYF"
     gene            7835..9082
                     /locus_tag="VGQ51_12135"
     CDS             7835..9082
                     /locus_tag="VGQ51_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV9037370.1"
                     /translation="MDLLRFITAGSVDDGKSTLIGRLLYDSKSILTDQLEALEKQSRN
                     RNKEAGEIDLALLTDGLRAEREQGITIDVAYKYFSTPRRKFIIADAPGHIQYTRNMVT
                     GASNANLIIILIDARNGVVEQTRRHSIIASLLNIPHVVVAVNKMDLVGYSQDRYNNIL
                     IDFAETARSLQLKDVTYLPISALKGDNIVERGSQLPWYDGPTLLGLLETTPVDIDTDL
                     RRGRLSVQYVIRPQTDEHHDYRGYAGKISSGIYRKGDTVTILPSGLTSRILAIETGGK
                     EVEEAFAPQSVILRLEDDIDISRGDLIVNGVHPPQVGQEFDVLLCWMDTKPLQPGNKY
                     LLQLGSRTVRGLVKDIEYRLDVNTLEKLPSPGQAGLNDIVRASIRTAAALPFDGYNDL
                     PAGGGAILIDETSFVTVGACMIQ"
     gene            9090..9914
                     /gene="cobA"
                     /locus_tag="VGQ51_12140"
     CDS             9090..9914
                     /gene="cobA"
                     /locus_tag="VGQ51_12140"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEV9037371.1"
                     /translation="MRKDTIHIGKVIFAGAGPGDPELLTVKALRWLNRADVVIADRLV
                     SPEILRDYIPPDALLIPVGKQSHHADSTPQSVIDHLLVEHALQGKLVVRLKGGDVSIF
                     SNILDELTALTSHRIPYELVPGVTAALGAAAYAGIPLTARGFSTAVRFLTGYSPDNAD
                     DGWYRDLAQTTDTLVFYMSSAPLDTLIRRLLQQGIPEDRWVAVIEQATTPLQRVNALP
                     VHDYLPAAGGSRYASPALVIIGRVAALHSEFQWLTNSGSNEEYFPGLSSTKKIALC"
     gene            9908..11626
                     /locus_tag="VGQ51_12145"
     CDS             9908..11626
                     /locus_tag="VGQ51_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEV9037372.1"
                     /translation="MLTTPKMNMMKELIAGSSREELIWLSGYLAGVVAQGPGAVSAPA
                     AVAAPQSTAAEPSAAPLRITIAYGTETGNAKRLATDFAARAKKKGIQAKLVGLEQYRL
                     GDLTKEEYLLTVISTQGDGEPPAAAKKFYDHIHQNGLRLDRLKYGVLALGDTAYPLFC
                     KAGEDVDQQLQKIGGQRLLSLQKCDTDYESEASKWFDSVLQQLNHAPTLAAAPVVIAK
                     PHTKKTYTGAILTNTNLNDHGSGKQTWHLEIEAEGVEYQPGDSLGIVPENPAVLVEAI
                     IALTGADANQLLPHRSEEWPLAHLLRRRLNIVYLPERVVKKYAGIVQQDIPETKIGLL
                     DLLKIYPVHDTRQFHAVVAILEPLAPRLYSISSSPEAHPGEIHLTVARDRFAVNGETK
                     FGLCSDGLSQLTPGQTIEFYIHPNSQFRLPAPDTDIIMIGPGTGIAPFRSFIAERDSR
                     GSQGKNWLFFGDRRFTTDFLYQTEIQDWAMTGVLTRVNTAFSRDQKEKVYVQHRMRQN
                     ATELFAWLQSGAHVFVCGAKQPMSVDVEDTLLSIIQKEGNKNAAQAQAYLDELKETGR
                     YVKDVY"
     gene            11616..13313
                     /locus_tag="VGQ51_12150"
     CDS             11616..13313
                     /locus_tag="VGQ51_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HEV9037373.1"
                     /translation="MSTEVNNMIENKNLSDTERIKQASDGLRGTLAETLLDELTGAIG
                     EDDKSLVKFHGMYQQDDRDRREERAAKKLERLWSFMIRLRIPGGLLTPEQYIALHHIA
                     GENSTGTIKITTRQTIQLHGLLKHRVKPTISAFNAAGLDSISACGDVNRNVTCAAHPM
                     QSAIHEQVHSYAARISRMLLPKTKAYYEIWLDQEKLTDKKEEEDPLYQDRYLPRKFKI
                     GIGIPPNNEVDVLTNDLGLIAVINNDRLEGFNVAIGGGLGATHGNPDTYPRLATVIGF
                     VPADDDRLFKTIYEIITIQRDYGNRSDRKLSRLKYTLDRLGADWYKQELTRRTGFELE
                     PPRPYFFNERKDLYGWQQNHQGLWYYTPFIENGRILDDEKLALKTALFEIAQTGKANF
                     RFTTNQGLILADIRKKDKNLVNGILEKFGILAHTETASAIRKNSIACVALPTCPLALA
                     EGQRYLPTLIGHIEVLLAAHDLSNEEMIIRMTGCPNGCGRPYAAEIGFVGTAPGRYNL
                     YLGGDHEGTRLNRLYKENLDEAAILRELGLLFWLYKKERITGERFGDFAFRQRWYKI"
     gene            13389..14606
                     /locus_tag="VGQ51_12155"
     CDS             13389..14606
                     /locus_tag="VGQ51_12155"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEV9037374.1"
                     /translation="MKRTTLLLSVLLATLASGAQTTNDLLKLLTQKGTITQQEADSIR
                     RDFSARQRKEEQTADSFPLRLGRSLDLSGYTQAVYQNFQHPGKYFDGFSIKRARLDFQ
                     GHFASQFDYRLLVDFAGASATSGTAPTGGALISPTLIEANITYKPFHFLQIRAGQLYV
                     AFSQENMTQDRNLDLIERSQVVSALVGRKGDAANGLVDSVGNQNGRDIGIQAAGSFFR
                     LRDAYFLDYTVQLLNGAGINSPDNNASRDIDARLVIHPAKFLSLGGSYYNGVDRFMTS
                     PAKDQKRIRYGGELAVNLSSLSLKGEYIRGQEGSGSAADHGTDPAITIHEGWYAQIGY
                     FLLPRKLQGVFRYDYYDPNTAKTQTASTYYDFGVNYFFNVWTKIQVYYSLRNQEGPAI
                     NNNLFEAQLQLAF"
     gene            14625..16154
                     /locus_tag="VGQ51_12160"
     CDS             14625..16154
                     /locus_tag="VGQ51_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEV9037375.1"
                     /translation="MELLMEAKNVTTNNLFPVFIKLERLRLLVVGGGNVCLEKLHAVL
                     QNSPATQITVVAPSINSAVRELADRHNNILLVERVYDPGDLDHADIAIVAVNDRSVSE
                     LVAQDAREKGILVNVADTPELCDFYLSSIIRKGNLKIAISTNGKSPTIAKRLKEEIGG
                     MIPDEMESVLDNMQTIRQGMNGDFSEKVRRLNDLTKVLVAKQATPELPARSRHSKWQQ
                     VVKWCLFAFFFMILGHGILSLVPFGGITDFFRGLPGGFDLETFGIMTLAGFLAQMVDG
                     SMGMGYGTISTVFLLAYGVSPAIVSSRVHSARVFSSGVSGYSHHRFGNINRKLFRTLV
                     VPGIIGAILGATLAYFGKEYSKYVRIPLSLYTIYLGWYILRKAFKRRNPKDKVKGAGW
                     LASAGGFMDAFAGGGWGTLVTSTLIAKRRNPRYVIGSVCLAEFFVVLSSAITFFILLK
                     SIPLLDVAGMILGGVLAAPLAARLVGKLPVKTMFIAVGTLVVLTSCSTLWKSLMLVLH
                     H"
     gene            complement(16148..17146)
                     /locus_tag="VGQ51_12165"
     CDS             complement(16148..17146)
                     /locus_tag="VGQ51_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HEV9037376.1"
                     /translation="MTKYTGIPATGDLFSLSAEVFIPRALELFDFQYGNNPLYRRYTE
                     ALGVDPAGVADIAAIPFLPVNFFKTQPVKTTEFIPEDVFTSSGTTGMIASRHEVKDLG
                     LYKRSFTAGFQRSYGPPREWCIIGLLPSYLERANSSLVSMVHELIGLSGHPDSGFYLY
                     DHEGLFGTLQRLESQAQKTLLIGVSFALMDFAGKYSMRLRHTVVMETGGMKGRRREIT
                     RPELHACLCERLGVDVIHAEYGMTELLSQAYSPGQGLFTSPPWMRVLVRAEDDPLDVR
                     TEGEGIINIIDLANCWSCAFLATEDVGRVFPDGRFEVNGRVDNSDARGCSLLVASQ"
     gene            17223..17666
                     /locus_tag="VGQ51_12170"
     CDS             17223..17666
                     /locus_tag="VGQ51_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037377.1"
                     /translation="MKPGYFLLPVLSFLLVLGSCKKSSTGTHPRITFASINTKIQPND
                     SMIGVFNFDNSGGTLGKGTFYSIRIRLNQAPPFILAGPDTLTASIPDFSGASKGQFRY
                     VLDWNDYLSSSGHINDTLQFKFFAMTPDSVYTDTLTSPEIIVINP"
     gene            17690..>18196
                     /locus_tag="VGQ51_12175"
     CDS             17690..>18196
                     /locus_tag="VGQ51_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037378.1"
                     /translation="MFRKIADYFVFSSLYIAFCAVLMTWQTSQLLLGSPPPMRLLGFV
                     FFATICSYNFHWYLTPRSSAPSRRVHWTNHHKALHFVLYLTGLVGAGVYFIYLWHWFF
                     ALCFAVFLTFLYSAPKVPHPLFRRLREIAIGKTIFLSFVWVYVTTVLPVIVAEVPWNL
                     SFVLFIAGR"
BASE COUNT         4238 a         5722 c         4553 g         3683 t
ORIGIN      
        1 ggtacggggt ttgcaataat agatcagcaa cgatgcaata ccgtaacaaa gtccgatccg
       61 ctgcagcact cccatgatcc ttgtgtgcga gataggcgcc agaccccaat gtccatccgc
      121 ctgctgacga aagaaaggga accagtaaag caggtatccg agcaaaaaga tcagcagcgt
      181 acgccggatg atcttccaga gtacggctcc ctgattcatc gtggcgaatt tcttcatcga
      241 aaagctcatg gcgttgccca ccgcgaacag aaaagacgga aagacaagat cggtgggtgt
      301 gaatccatgc caatgcgcgt gtttcagggg agagaaggca aacgtatcgc tacccggcgt
      361 attgacgatg atcatgaagc atacggtcat cccccggaat acatccaggg ccaatgatct
      421 ttggggttta tcattcatat atgagcaaga aaggcgttct ttggcggctt ttggcctgaa
      481 gatactaaaa ataaaatatt tgcatgcttt caacgtttta gaagagcgca acccacccca
      541 aaattttgaa aaccaaaacc ttacatagtg cttatcgcct cttaccgctc ttcgggtcac
      601 tactgttgct ggtttcttcc ctgtctgccc agcccgtgag acaaaaccgg cccggggtcg
      661 tccgtctgaa aaacggcgtt atcaacgtcc ggcggaatat cgccgatggc tcgcttcgac
      721 gggattccct tctgaccgca catttcaaaa gacattatta tgtcctggca cagtttgacc
      781 gggtgcccga taccaacctg cgcgcagaaa tggcgcacac cggtctgcat ctctacgact
      841 atgtctccga taaggcctgg ctggtcgagc ttggcgacag cttttccatc gcacagctgc
      901 aacggtttgc tatcggcgga cttaccgcgc tatccccacc ctccaagatc agccgccgtc
      961 tgcaggacca ccgggatgaa gacctgcatg accccgacaa ggccatagcg gtcggatatt
     1021 tcgggaccct ttcgcccgac caggtacgac aggaaataac cgcaaccggc gcgatcatcg
     1081 tacccagcaa gctccagccg ccccgcgttc tcttcgtccg gatcccaaac ccggcaatcc
     1141 tgcaacgcat ctccgccctt cccttcgtca gctatatcac cacgcaaccc atcaaaccgc
     1201 ggacgctcaa ctacaacaac cgtgctgcgc aaggacccga cgcgctcgcg gcaagccgca
     1261 atctgtatgg cgacggcgtc gtcgttggtg tgggtgacga taccgatccc tatacccatg
     1321 tggacttcac aggccgggag atcgaccggt tcgatggccc tccgggttcc ggtcatggcg
     1381 tacatacctc cgggatcgta ggcggcggcg gcatcatcaa cccgatgtgg cagggcatgg
     1441 cgccgcattc cattatcctc agccagttct tcagcgatat cctggtcaat accccggcct
     1501 atatgaccga ttacgatatg ccgctgacca gcaattcata tacggactat gactacggtt
     1561 gccaatacga cggcctctat gacgcgctgg ccaactatac ggatgcacag ctcctcgcat
     1621 atcccaacct gatgcacgtc ttcgccgccg gcaatgacgg cggttatacc tgcacaccct
     1681 tccctccaca attcgctacg atcaaatcgg gtttccaatc cgccaagaac gcgatcagcg
     1741 tcgggaatgt cgacaatact aacccctacc acgacaacac ctatagcatc aacagcggat
     1801 caagcagcgg tcctaccgat gacggtcgta tcaaacccga tctcgtcgca ggaggctcgg
     1861 ccatcacttc gacgcttcct tataattctt acggacagga atggggcacc agcatggcct
     1921 gcccaacggt cgcagggact ctcgcgctgc ttgtacaacg ctaccgccag caacacggcg
     1981 gcgccgatcc ttccgccgcg ctcctcaagg cactgatctg taacacagcc accgatctgg
     2041 gtaaccccgg acctgattac ctgtatggat acggatcgct cgacggcaaa gccgcggccg
     2101 atgccatgga tgcggtgcag tattctttcg gcacggtctc cgacggcggt accgccaatg
     2161 ccaatcttat cattccgccg ggtctcgcgc aggttcgcat catgatctat tgggccgact
     2221 accccgctgc cccatatgcc gccactgcgc tcgtcaacaa tctcgacctc acggtgacgg
     2281 acccgtctgc ggtgctgcat catccgctga tcctgaatcc cgatcccgca catgtcaacg
     2341 acaatgcggt cgaaggcgtc gacaacgtca ataatatcga acaggtggtg ctcaacaatc
     2401 cttccggcgg cacctgcaat atcgccatcc acggcaccag catcccgcag ggcccgcagt
     2461 cttatgtgct tgtctaccag ttcatccaac cctccgtggt cctgctctat ccttacggca
     2521 gggagacctg gatccccggc tactatcccg aatacatccg ctggaacgcc ttcgacaaca
     2581 gcccgaatac gttcacgctc gactactcca cggacaacgg tgccacctgg accacgataa
     2641 ataatgcggt cagcgccgcc agccgtatgt atatgtggac cgcgccgaat gtggccacca
     2701 accaggccct tgtacgcgtc acccgcaatg gcacatcgta cagcagcgtc agcacatatc
     2761 cttttactat cctgggccaa ccctcggcct ccgtgaccaa tccctgccag ggctatgtgc
     2821 agctcagctg gaacactatc ccgtcggcaa caagctacga tattatgcag ctggtcggcg
     2881 atacgatggt aaaggtcgcc agcacgacga ccacgagcta tctgctgggt catctcaacc
     2941 gcgacagcag ctattggctg ggcgtacgcg ccgtgaacgg cacatccccc ggacgacgct
     3001 ccatatcggt caatatcact ccttcgggcg gcgcatgtaa cctgagcgcg ctcgacaacg
     3061 actattccgc ggactcgctg atcggtctta attccggccg gatgtatacg tctacgcaac
     3121 tgacatcggc tgtccccatt gaggtcgaac tgaaaaacct gggcacgatc cctacgggat
     3181 ccgccttcac ggtttcctat agcatcaacg gcgcgacgcc cgtcaccgag gccagcagtg
     3241 ccgtcattcc gcccaacaac ggagcctttg actatacgtt ttccacgacc gccgatctct
     3301 ccgcccccgg cagctatacg gtgcaggtgt gggtatccta tcccggtgat ccggcgacgg
     3361 gcaacgacac tgtgacggcg gtgatcaaac agctgaccaa cgacccggtc acgctcaacc
     3421 ctgccatgac ggaaggtttc gaaagcgccg cggctgcgag ctatgtttct cccacgatgg
     3481 gtttcacggg actcgaccgc tgcgatttca tgacaagcaa tggcaacggc cgtgtacgca
     3541 ccttcgtcaa taccggtatg gcccactctg ggaatcgata tgccgtgctt gaccagacgc
     3601 attatgccac ttcactcact tccgacagtc ttataacgac tttcaatctc tccgcctatt
     3661 cctccaccga tcagatctgg ctcgacttct ggtaccggaa tcagggaagt actgcgaacc
     3721 tcggcggcaa caccgtctgg ataaggggca acgatcagtc ggcctggata tcggcttacc
     3781 tcctcgatac gacgaccgcc aacataggca tttaccaacc ctcttcgcat gtcaatgtca
     3841 cccagctgct gaacaacgca tcggaaacca tcggcagcag cttccaggtc aaattcggag
     3901 aagtaggcaa taccgggggc agcgacgtca ttaccgacgg gactctcgac aatggctata
     3961 ttttcgacga tatcacgctt acaaaaggca ccaatgacat cggcgtgctg agactggtct
     4021 cccccgtcgc tacgggcagc tgctcgctac ccggcgccac ccccatcggc atcggcgtaa
     4081 ggaattacag caacaccgcc gcggtgaata tcaaggtctc ttatgcgatc gatggcgata
     4141 ccgtcaccga aaccatcccc tccatcggcg ccggcgattc catcgtctat accttctccg
     4201 cgacgaaaga catgtccgcc ttccgcgcct ggcggatcga cgcctgggtc agctataccg
     4261 gtgatactta ttcgctgaac gacaccctgg ccccgggcac catccaaacg acgccgctca
     4321 tctctgcctt cccctatctg gagagcttcg ataccagcaa cggctattgg tatacgggag
     4381 gcatcaattc gagctggcaa tggggcgcgc cgaaaaaatc cgtcatcgac aaggccgcga
     4441 gcggtcccga ttgctgggtg accagcctga cgggcaacta taacaataat gagcggtcct
     4501 atctctattc cccctgtttc gatctcagcg gacttacctc ccccgtcctt tccttcagcc
     4561 atattttcca gacggaggac gattgcgact gcgactatca ttgggtcgaa tataccacgg
     4621 acgatgtgaa ctggagcagg ctgggcgccg tcggagcggg aaccaactgg tatgacaata
     4681 cgaccaggca ggcctggcag ctttcctggt ctaaatggca tgtatccagc tacgacatcc
     4741 cggtcaatgc gccgaaggtc aggttccgca tcgtgatgaa cagtgacccg ggcactactt
     4801 atgagggtgt cggcatcgat gatgtccaca tattcgacaa ggcttccgtt tataatggcg
     4861 ccgacgacag cgtcacgaag tccgtcagcg gcaacggctg gatcaatttc gacatcggcg
     4921 gccgacgcat cgcggctatc gatcccaatg gccaggacct cggacccacc accgtcaagg
     4981 tcttcttcaa ccatacgggc gcggtccggc atgataccgt ccagtactat ctcgaccgca
     5041 acctcgtcat acaaccctcc aatccgccgg ttgccaatgt gggcgtccgg tattatttcc
     5101 tcgacagcga ggcggtagcg ctgatcaatg cgtcgggatg tctctcctgc aacacgatcg
     5161 ccgatgccta ccaatccggc gtggcgcaat tcagcagccc ggcagccgcc gaggaagaca
     5221 gcactctgca gaacgacagc accggtgaat tccggttcca tcatccgcac cgggatgtcg
     5281 ccatcattcc caacgacaat ggctactatg cggaatacca ggtaggcggc ttctccgaat
     5341 tctggatctg cgataccatc ccgccggaga ccgcatcccc tacgctgacg ctgctctcct
     5401 tcaccgcgac aagggcgggc aataccgccc tcctgcaatg gagcaccgcg aatgccttcg
     5461 gcatcgacca ttataccatc gaaaaaggta ccgacagtat ccatttctct ccgctcgatt
     5521 cgctgcccgc cgcaaccgac ggccgcggaa cggacaatta ccagtatacc gacacccatc
     5581 tcgacagcgg cgccaactac taccgcctgc gcgcggtgga tgccttcggc aacttcatcg
     5641 ggtcacctat ccggaaagta gagggccccg acagcgtgag cgtcatcatt tatccaaacc
     5701 cggtccaccg aacctacgtc tttatcagca cgacccggag cgcccggctc atccggctga
     5761 tcgatgtttc cggcagaacg gtgctggtgc gttctgtacg cggcaatctc aataccgtgc
     5821 cgctcagtgc gctggcccca ggcatctatt tcgtcgaagt caccaccgat acggggtcta
     5881 cagtgcagaa aatcctcatc cggtaacttt ccgcgacccc gtggtccaaa actttttttg
     5941 tttttccggc gaacggctac tatctttgtc cacaataatg gtagacaata gtatgaacac
     6001 gcatctcccc tccatccggc atcagatttc ctccatgtcc agcagcgaac cctgttgttg
     6061 ctgttgatcc ctcatctacc cggggcctaa gaaacaattc ccttcatcac atcaccggat
     6121 catggaccac acacaagaca taaaacaagc ttctgccgaa ttactctacc taattagtgg
     6181 attatccatc agcgaggcgc ttgcgcttct ggtcaaacga tatccgggta aggtcacctt
     6241 ctccacgagc ttcggaatcg aagaccaggt catcacccac catattctga gtgccgggtt
     6301 gccggtatcg atcttcacgc tggataccgg gcggctcttc gcggagacgt attccgtatg
     6361 gagcgccacc aacgaaaaat acgggaccag gctgaaggcc tattaccctg accgacggtt
     6421 gcttgaatcg tacctggaag aaaaaggacc caacgccttt tacgagtcgg tggccaaccg
     6481 caaagaatgc tgtcatatcc gcaaggtaga gccgctgcaa cgggccctcg cgggtcaaac
     6541 ggtctggatc accggtctaa gggccgaaca atctccccaa cgggcagacc accagatcat
     6601 agaattcgac gaaggcaaca agatcattaa atataacccc ctgctgcatt ggagcagcga
     6661 cgaggtcagg gacttcatcg atcagaacaa tattccctac aacccgcttc acgataaggg
     6721 tttcgtcagc atcggctgcg ctccctgtac acgcgccata cggccgggcg aagattttcg
     6781 ggccggaaga tggtggtggg aagacaacag taaaaaagaa tgcggcctgc atgtcgcgtc
     6841 atagcggccc gcatgtcgca tcatagccgc ccgaacgcgg catcataaaa aaatgatatg
     6901 agcaacgaca ccaaatacca gttagactat ctcgaccagt tggagtcgga gtccatccac
     6961 atcctgcgcg aggtggcggg acagttcgaa catccggccc ttcttttcag cggaggcaag
     7021 gactccatca cgatggtcca cctggcctta aaagcctttc gcccggggaa gttccctttc
     7081 ccgctggtgc atatcgatac cggtcataat ttccctgagg cgctggactt ccgcgacagg
     7141 ctggtatcca ctatcggcga aaagctgatc gtccgcaagg tggaagacac catacgccgc
     7201 cggcatctca ccgagcccgg tggaaaattc tccagccgca acgccctcca gtcctatacg
     7261 ctgctggata ccatcgagga gttcgggttc gacgcctgca tcggcggcgc ccgccgtgac
     7321 gaggaaaaag cacgggcgaa agaaaggata ttctccgtcc gcgacgagtt cggcggctgg
     7381 gatcccaaac gccagcgtcc cgagctgtgg aatatctaca acggccggat ccgcaaaggc
     7441 gagaacgtac gggccttccc catcagcaac tggaccgagc tggacatctg gaactatatc
     7501 cgccgcgaga acatttcgtt accctccatc tatttcgcgc acgaacgcga ggtcatcgac
     7561 tatgaaggac aatgggtggc cgtttcgccc tttatccgtc tggactcagg cgacagcgta
     7621 caacgtcgca cggtccgcta ccgtacggtc ggcgatatga cctgcacggc cgctgtggag
     7681 tcatcggcat cggtcatcga cgatatcatc cgggagataa cggctacgcg caccagcgag
     7741 cgtggcgaga cacgcatcga cgacaaggta tcggaagccg cgatggaaga cagaaaaaag
     7801 aatggctatt tctaacgatc aacaagctca acacatggac ttattacgat tcataaccgc
     7861 aggtagcgtg gacgacggga aaagcaccct catcggaagg ttgctctatg acagcaagag
     7921 catcctgacc gaccagctgg aagccctgga aaaacagagc aggaacagga acaaggaggc
     7981 cggagagatc gatctggccc tgctgaccga tggtctgcgc gccgaacgcg aacaaggcat
     8041 caccatcgat gtagcctata aatatttttc gacgccaagg cgcaagttca tcatcgccga
     8101 cgcgccgggt catatccagt acacccgcaa tatggtgacg ggcgcaagca atgccaatct
     8161 aatcatcatc ctcatcgacg cccgcaacgg tgtcgtggaa cagacccgca gacattccat
     8221 catcgcttcg ctgctcaata tcccccacgt ggtcgtcgcc gtcaacaaga tggatctcgt
     8281 gggctattcg caggaccgct ataacaatat cctcatcgat tttgcggaga cagccaggag
     8341 cctgcagctg aaagacgtca cctatctgcc catcagcgcg ctgaaaggcg acaatatcgt
     8401 tgaacgcggc tcgcagctgc cctggtacga cggtccgacc cttctgggtc tgctggaaac
     8461 gaccccggtc gatatcgaca ccgatctgag acggggcagg ctcagcgtcc agtacgtcat
     8521 ccgtcctcag accgacgagc accacgacta tcggggctat gccggcaaaa tatccagcgg
     8581 catctaccgg aaaggcgaca ccgtcaccat cctcccttcg gggctgacca gccgcatcct
     8641 ggccatagag accggcggga aggaagtaga agaggcattc gccccgcaaa gcgtgatcct
     8701 tcgtctcgaa gacgacatcg atatcagccg cggcgatctc atcgtgaatg gcgttcaccc
     8761 accccaggtg ggacaggagt tcgacgtttt gctttgctgg atggatacca agccgctgca
     8821 gccgggcaat aaatacctgc ttcaactggg tagccgtacc gtacggggat tggtaaagga
     8881 catcgaatac cgcctcgacg taaataccct ggaaaagctg ccttcgcccg gacaagcggg
     8941 tctgaacgat atcgtcaggg cgagtatccg caccgccgct gccctgccct tcgacggcta
     9001 taacgatctg cctgcgggag gcggagccat cctcatcgac gaaaccagct ttgtaactgt
     9061 aggcgcctgc atgatccaat aaaaagagaa tgcgaaaaga tactatccat atcggtaaag
     9121 tcatttttgc gggagcagga cccggtgatc ccgagctcct cacggtcaag gcccttcgct
     9181 ggctgaaccg cgcggacgtc gtcattgccg accgcctcgt aagcccggag atcctccggg
     9241 actatatccc gcccgacgcc ctgctcatcc ccgtcggcaa acaatcgcac cacgccgact
     9301 cgacaccgca atccgtcatc gatcacctgc tggtcgaaca tgccctgcaa ggcaagctcg
     9361 tcgtccggct aaaaggaggc gatgtctcca ttttctccaa tatcctcgac gagctgacgg
     9421 cgctgaccag ccaccggatt ccctatgagc tggtacccgg cgtcaccgct gccctcggcg
     9481 cggccgccta tgccgggata cccctgacgg cgagaggctt ttccaccgcc gtacgtttcc
     9541 tcaccggcta tagccccgac aatgcagacg atggctggta ccgcgatctg gcgcaaacaa
     9601 cggataccct tgttttttat atgtcttcgg ccccattgga taccctcatc cggcggttgt
     9661 tgcaacaagg catacccgaa gaccgatggg tagccgtcat cgaacaggcc accactccgc
     9721 tccaacgcgt gaacgccttg cctgtgcatg attatcttcc ggccgccggt ggaagccgct
     9781 acgcttcccc ggcactggtt attatcggcc gggtggccgc ccttcactcc gaattccaat
     9841 ggctgacgaa cagcgggagc aacgaagaat attttcctgg gctttcatcc acaaaaaaaa
     9901 tcgcgctatg ctgacgacac cgaaaatgaa tatgatgaag gagctgatcg cgggctcttc
     9961 ccgcgaagag ctgatctggc tcagcggcta tctggccggg gtcgtcgcgc aaggaccggg
    10021 tgccgtatcc gcccccgcgg ccgtcgctgc accgcaatcc accgcagccg agccttccgc
    10081 ggccccgctc cggatcacca tcgcctatgg caccgagacg ggcaatgcca aacgtcttgc
    10141 gaccgatttc gccgccaggg ccaaaaagaa gggcatccag gccaaactgg tcggtctcga
    10201 acaataccgt ctgggcgatc tgaccaagga agaatacctt ctcacggtga tcagcaccca
    10261 gggtgacggc gaaccgcccg ccgccgcgaa aaaattctat gaccatatcc atcagaacgg
    10321 tctgcgtctc gatcgcctga aatacggtgt cctcgctctc ggcgataccg cctacccgct
    10381 gttctgcaag gccggtgaag acgtcgatca gcaattgcaa aaaataggcg gacaacgtct
    10441 cctctcgctt caaaaatgcg ataccgacta cgagagcgaa gcctcaaaat ggttcgattc
    10501 ggtgctccag caactgaacc acgcccctac gcttgccgcc gcacccgtcg tcatcgcaaa
    10561 gccgcacaca aaaaaaacat ataccggcgc catcctcacc aataccaatc tcaacgacca
    10621 cggctcgggt aaacaaacct ggcacctgga gatagaagcc gaaggtgtcg aataccaacc
    10681 gggcgatagc ctgggaatag tcccggaaaa cccggccgtc ctcgtcgagg ccatcatcgc
    10741 actcaccggc gccgatgcca accagctcct tccccaccgg tcggaagaat ggccgctggc
    10801 acacctgctc cgccggcgac tgaacatcgt ctatctcccc gaacgcgtcg tgaaaaaata
    10861 tgcgggcatc gtacagcagg acatacccga aacgaagatc gggctgctcg atctgctcaa
    10921 gatctaccca gtgcacgaca cgcgccagtt ccatgccgtc gtcgctattc ttgaaccact
    10981 ggcgccgcgg ctttattcca tttcctcctc acccgaggct caccccggcg agatccatct
    11041 cacggtggcg cgcgaccgat ttgccgtcaa cggagaaacc aagttcggcc tttgctccga
    11101 tggactttca caactgacgc cgggacagac catcgaattc tatattcatc ccaattccca
    11161 attccgcctc ccggctcccg ataccgatat catcatgatc ggacccggaa cggggatcgc
    11221 acccttccgt tcattcatcg cggaaagaga cagtcgcggg tcgcagggaa agaactggct
    11281 tttcttcggc gaccgccggt ttaccaccga tttcctctac cagaccgaga tccaggactg
    11341 ggcgatgaca ggagtgctca cgcgcgtcaa cacggctttt tcccgcgacc agaaagaaaa
    11401 ggtctacgtg caacacagga tgcgccaaaa tgccacggaa ctattcgcgt ggctgcagtc
    11461 cggtgcacac gtctttgtct gcggcgccaa acaaccgatg agcgtagacg tggaggatac
    11521 cctcctctcc attattcaaa aagaaggcaa caaaaatgcg gcccaggccc aggcctatct
    11581 cgacgagctg aaagagacgg gccgttatgt aaaagatgtc tactgaagtg aacaatatga
    11641 tcgaaaacaa gaacctgtcg gataccgaac ggatcaagca agccagtgac ggccttcgcg
    11701 gcacccttgc cgaaaccctt ctcgacgagc ttaccggggc catcggcgag gacgacaagt
    11761 cgctggtgaa attccacggc atgtatcagc aggatgaccg cgaccgccgc gaagaacgcg
    11821 ccgccaaaaa gctggaacgg ctttggtcct tcatgatccg tctgcgcata cccggaggtc
    11881 tcctgacccc cgaacaatat atcgcgctgc accatatcgc cggagagaac tccacaggca
    11941 ccatcaagat cactacccgt cagaccatcc agctgcacgg tctcctgaaa caccgggtca
    12001 aacccactat cagcgccttt aacgccgccg ggctcgactc catctccgct tgcggagatg
    12061 tcaatagaaa cgtcacctgc gccgcccacc cgatgcaatc cgccatccat gaacaggtgc
    12121 acagctacgc cgcccgcatc agccggatgc tgctaccgaa gacaaaagcg tattacgaga
    12181 tatggctcga ccaggaaaag ctcaccgata aaaaagaaga agaagatccg ctgtaccagg
    12241 accgctacct tccgagaaaa ttcaagatcg gtatcggcat cccgccgaat aacgaggtcg
    12301 acgtactgac caacgatctc ggactgatcg ccgtcattaa taacgatagg ctcgaaggct
    12361 tcaatgtcgc catcggcggg ggtctcggcg ccactcacgg aaacccggac acctatcccc
    12421 gtcttgcaac ggtgatcggg ttcgtccccg cagacgacga ccggttgttc aaaacgatct
    12481 atgagatcat caccatccag cgcgattatg gcaaccgcag cgaccgcaaa ctctcccgtc
    12541 tcaaatatac cctcgaccgt ctcggcgccg actggtataa acaagaactt acgcgacgca
    12601 cgggattcga gctggaacca ccgcgtccat atttcttcaa cgagcggaaa gatctttacg
    12661 gctggcaaca aaatcaccag ggcctctggt attatacgcc cttcatcgag aacggcagga
    12721 tccttgacga cgaaaaactg gcgctcaaga ccgcactttt cgagatcgcc cagaccggca
    12781 aggccaattt ccgattcacg accaaccagg gcctcatcct ggccgacatc cgaaaaaaag
    12841 acaagaacct ggtcaacggt atcctcgaga agttcggaat cctcgcccat accgaaaccg
    12901 cctctgccat ccgtaaaaat tcgatcgcct gcgtcgcatt gccgacctgc ccgcttgcgc
    12961 tggccgaagg gcaacgctat cttccgacgc tgatcgggca tatcgaagtc ctgctggctg
    13021 ctcacgatct ctccaatgaa gagatgatca tccggatgac cggctgcccc aacggctgcg
    13081 gacggcccta cgccgcggag atcggcttcg tcgggacggc cccgggacgc tataacctct
    13141 atctcggcgg ggaccatgaa ggcacgcggc tcaaccggct ctataaggaa aacctcgatg
    13201 aagctgcgat cctgcgcgag ctggggctgc tattctggct gtataaaaaa gaacgcatca
    13261 ccggcgagcg gttcggcgat tttgccttcc gccagcgttg gtataaaatt taaacggaca
    13321 cgggatcgcc tgccccatcg gcggcgatct tttttattaa ccaattactc tactaaattt
    13381 gtagactaat gaaacggacc acactgcttc tctcggtact cctggcaacg ctggcctcgg
    13441 gcgctcaaac caccaacgat cttctgaaac tgctgaccca aaaaggcacc atcacccaac
    13501 aggaggcgga ttccatccgc agggacttct cggcccggca aagaaaagaa gagcagacgg
    13561 cggattcttt cccgctccgc ctcggccgct cgctggacct cagcggatat acccaggctg
    13621 tctatcagaa tttccaacac cccgggaaat atttcgacgg cttcagcatc aaaagagccc
    13681 gtctggattt ccagggccac ttcgcgtcgc agttcgatta tcgcctgctc gtcgatttcg
    13741 caggcgccag cgcgacatcc gggactgcac cgaccggcgg ggccctgatc tcccccaccc
    13801 tcatcgaggc aaacatcacc tacaaaccct tccatttcct ccagatcagg gccggacagc
    13861 tatacgtcgc tttctcgcag gaaaatatga cacaggacag aaacctcgat ctgatcgagc
    13921 gctcgcaggt cgtcagcgca cttgtcggac gtaaaggcga tgcggccaac gggctggtgg
    13981 actccgtcgg caaccagaac ggaagggata tcggcatcca ggcggcagga agcttcttcc
    14041 gcctgcgaga cgcatacttc cttgactata cggtccagct gctcaacggc gcgggcatca
    14101 attcacccga caacaatgcg tccagggaca tcgacgcccg actggtcatc cacccagcga
    14161 aattcctgag cctcggcggc tcgtactaca atggcgtcga ccggttcatg acttcgccgg
    14221 caaaagacca gaaacgcatc cgctacggcg gcgagctggc ggtgaatctt agttcgctgt
    14281 cgctgaaagg agaatacatc cgggggcagg aaggcagcgg ttctgccgcc gatcacggga
    14341 ccgatcccgc cattacgatc catgaaggct ggtatgccca gatcggctac ttcctgctgc
    14401 cccgcaagct gcagggtgtc ttcaggtatg actattacga tcccaatacg gcaaagacgc
    14461 agaccgcctc gacatattat gacttcggcg taaactattt cttcaacgtc tggaccaaga
    14521 tccaggtata ctattcgctg cggaaccagg aaggtcctgc catcaataat aatctgttcg
    14581 aagcgcagct tcagcttgcg ttctgacaat aatctactaa ttttatggaa ttattaatgg
    14641 aagccaagaa cgttacaacg aacaatctgt tcccggtatt cataaagctg gaacgcctgc
    14701 gtctgctcgt cgtgggtggc ggcaatgttt gcctggaaaa gcttcacgca gtattacaaa
    14761 attcacccgc aacacaaatc accgtcgtcg ctccgtctat taattccgcc gtcagggagc
    14821 ttgctgatcg gcataacaac atccttctcg tcgaacgcgt ttatgatccc ggcgatctcg
    14881 atcatgcgga tatcgccatc gttgccgtca atgatcgttc cgtcagtgag ctggtcgcac
    14941 aggacgcgag ggaaaaaggc atactcgtca acgtggccga tacgccggaa ctctgcgatt
    15001 tctatctctc gtccatcatc cggaagggta atctaaagat cgcgatctcc accaacggca
    15061 aatccccgac tattgcaaaa agactgaaag aagaaatagg cgggatgatc cccgacgaaa
    15121 tggaaagcgt gctcgacaat atgcaaacca tccggcaggg catgaacggc gatttctccg
    15181 agaaggtacg tcgtctcaac gatctcacga aggtgcttgt ggccaaacag gccactcccg
    15241 agcttccggc ccgttcccgg cacagcaaat ggcagcaggt ggtcaaatgg tgtctcttcg
    15301 ctttcttctt catgatcctc ggacacggca tcctgtccct cgttcctttc ggcggcatca
    15361 ccgacttttt ccgtggtctg ccgggtgggt tcgatctcga gaccttcggc atcatgaccc
    15421 tcgcaggttt cctcgcacag atggtagacg gctcgatggg tatgggatac gggacgatct
    15481 ccaccgtttt ccttttggcc tacggtgtaa gcccggccat cgtcagcagc cgcgtacact
    15541 ccgcgcgcgt tttctccagc ggcgtttcgg gatacagcca ccaccgcttc ggaaatatca
    15601 accgcaagct gttccgtacc ctcgtcgtcc ccggcatcat cggcgccatt ttgggtgcta
    15661 cgctggcgta tttcggtaag gaatattcga aatatgtgcg gatcccgctt tctctctaca
    15721 ccatctatct cggctggtat atcctccgca aggccttcaa acgacgcaac ccgaaagata
    15781 aagtgaaagg ggcgggctgg ctggcatcgg ctggaggctt catggatgct tttgccggcg
    15841 gcggctgggg cacgctcgtg accagcacgc tcattgccaa acgaagaaac ccacgttatg
    15901 tcatcggctc cgtctgtctc gccgaatttt tcgtcgtcct gtccagcgcc atcacttttt
    15961 tcatccttct gaaaagcatt ccgttactgg atgtcgcagg catgatcctc ggaggtgtgc
    16021 tcgccgcacc gctcgcggcc cggctggtcg gcaaactgcc ggtcaagacc atgttcatcg
    16081 ccgtgggcac cctcgtcgtc ctgaccagct gtagcacact ctggaaatcg ctgatgctcg
    16141 ttctgcatca ctgagaagct accagcaggc tgcaccccct tgcgtcgcta ttatccacgc
    16201 gtccgttcac ctcgaaccgg ccgtcgggaa agacccttcc cacatcctcg gtcgccagga
    16261 aagcacatga ccagcaattg gccagatcga tgatattgat gatcccttca ccctccgtcc
    16321 ggacatcgag aggatcgtcc tccgcgcgga ccagcacccg catccacggc ggagatgtga
    16381 acagcccctg ccccggcgaa tacgcctgtg acaacaattc cgtcatccca tactcggcat
    16441 gaatgacgtc tacgcccagc cgctcgcaga gacaggcatg cagctccgga cgcgtgattt
    16501 cacgcctacg ccccttcatt ccgcccgtct ccatgaccac ggtatgccgc agccgcatcg
    16561 aatacttccc ggcaaaatcc atcagcgcga aactcacccc gatgagcagg gttttttgcg
    16621 cctggctctc cagacgctgc agcgtcccaa acagtccctc atgatcgtac aaataaaagc
    16681 cgctgtcggg atgccccgac aaaccgatga gctcatgcac catcgagaca agcgatgaat
    16741 tggccctctc caggtaagaa ggcaacagcc cgatgataca ccactcccgc ggcgggccgt
    16801 acgaccgctg gaagcccgcg gtaaaactcc ttttatacag cccgagatcc ttgacctcat
    16861 gacggctggc gatcattccc gttgtaccac tactggtaaa tacgtcctcc ggtatgaact
    16921 cggttgtctt taccggttgc gttttgaaaa aattcacggg taaaaaggga atagcggcga
    16981 tatcggcaac ccccgccgga tccactccga gggcctctgt gtaccggcga taaaggggat
    17041 tattcccata ctggaaatcg aaaagctcca gcgcccgggg gataaaaact tccgcggaaa
    17101 gggaaaaaag atcgcctgtg gcaggtatgc cggtatattt agtcaatgaa ttgcaaaaac
    17161 tccggcgaaa ttaccccctt ttagttaaat ttaggtaggt ttgtacttat caaatcagct
    17221 acatgaagcc tggttatttc ctgctacccg tcctgtcatt cctcctcgtc ctgggaagtt
    17281 gcaaaaaaag ctcgaccggc actcatccca ggataacctt cgcgtctatc aacacgaaga
    17341 tacaacccaa tgattcgatg atcggcgtgt tcaacttcga taacagcggc ggcacccttg
    17401 gcaaaggcac tttctattcc attcgcatac gactgaacca ggctccgcca ttcatcctgg
    17461 caggacccga caccctcaca gcctcgatac ccgatttcag cggtgcgagc aaaggacagt
    17521 tccgctacgt tctcgattgg aatgattacc tcagcagcag cggccatatc aacgataccc
    17581 tgcagttcaa gttcttcgcc atgactcccg atagcgtata caccgacacg ctcacgtccc
    17641 cggagattat agtcataaat ccttaactta ccatacgatt caacaaagcg tgttcagaaa
    17701 aatagccgat tattttgttt tcagcagcct ttatattgcc ttttgtgccg tcctgatgac
    17761 ctggcaaacg agccagctgc tgctgggatc gcctccgccg atgcgtctcc tggggttcgt
    17821 attctttgct accatctgca gctataattt tcactggtac ctcacgccgc gctccagcgc
    17881 cccatcgcgt cgcgttcact ggacgaatca tcacaaggcc cttcacttcg tcctctacct
    17941 gaccggactc gtaggcgcgg gcgtatattt catttacctg tggcactggt tcttcgccct
    18001 ttgctttgcc gtttttctca cgttccttta ttcggcccca aaggtacctc atcccctctt
    18061 ccggcggttg cgcgagatcg ccataggaaa aaccatattt ctctctttcg tctgggtcta
    18121 tgtgacgacg gtactacccg tcatcgtggc ggaggtgccc tggaatctgt ccttcgtcct
    18181 ttttatcgcc ggccgg
//
LOCUS       DASYSV010000397         7546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2124877, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000397 DASYSV010000000
VERSION     DASYSV010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124877"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..2186
                     /locus_tag="VGQ51_12180"
     CDS             216..2186
                     /locus_tag="VGQ51_12180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9037379.1"
                     /translation="MKNDARFAVFFIILTLHWDIACGQTLSGTVNSYYSITAVNSAAN
                     AVTVDNASGLSVGQRVFLYQAKGASITATNTANYGDITAANNAGAYEFNIICTISGNE
                     IWMLNALANTYDPTAMVQLVTVPYSSSLTVGGTITPQPWDSLTGKGGIVVLEATGSIT
                     LNADINVTGQGFEGGTLINYAVPPYNCSWAVTVSAYYLSYPASGFNTGGRKGEGIAAY
                     ITGEEFGRGKLANGGGGGDNGNSGGGGGGNYGTGGAGGQRTGESTFDCHAQYPGVGGA
                     SVAAYGYSSAKNRIFFGGGGGSGEENNGYGEPGGNGGGIIVLSAPVIVGGGGRLLATG
                     VSPTNPICTDPYQAEGDGGGGGGAGGTIVLDASTITGTITADASGANGSNSSNHVGDC
                     TGPGGGGAGGMVWTAGSSFPGAVTATVSGGANGVVSSGSTKSPSCKGFANGATAGTAG
                     MTLSSFAPPVSSGPVCVALASPVLQYFNAMRTSQDIQLSWALVSPETATDMRNFILQR
                     SSDLSHFAGVATVSCSKDSISYHYTDRAANSNGTLDYRLAWQNSNGEWFYSRVVAVAG
                     VPGPDGSSIRLYPDPATNYLTLTVISSANESATVSISSALGQSLLTRPISLFKGLNTV
                     TVPIATLAPAAYFLVLRSGGNRIVKPFLKKNQ"
     gene            complement(2170..3651)
                     /locus_tag="VGQ51_12185"
     CDS             complement(2170..3651)
                     /locus_tag="VGQ51_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9037380.1"
                     /translation="MRTFRKIVILAVSLAAISAKSDAQGLGIELDGGLQGMQYKLPGG
                     HSTALPGGSLGLTYTFRVNSQWGVRTGILGSIYRTQASLKDGTYSSGQVDDGGSAFNY
                     NIKATGYKETQQFYAATIPLLLHFHTGGAGLEWYFDGGGKFIFPFNTSIQASARQLSL
                     AGYYPNFHIVVSNLPQHGFGTVNGWHAGSTTGLKPAGALSAGTGISFAISRHTRIYTG
                     VYVDYGLTDLREKSDSMPLVTYSTAGISHVKANGAMHLPNSGAATLLSFGVQVRLSFG
                     SATSPGRRKRTQRHETDSLRQASAAVSPAKNSPPRATEPVSRVPASLPPAKDSAVQVT
                     PKQASLPPAKDSTVAEAGATAVSTDNISLDSLIRRDEAQSAKRSQAERSRRNVVFGII
                     GETAIPDMQKAKLDELVSLLKDHPDLRLSIEGHFCDSLMQMENSQTAETRMKAVVKYL
                     ENKGISRRRMDIGYLKKSDPVLSYDPAANYRNRRVAIHIIGSF"
     gene            complement(3662..5800)
                     /locus_tag="VGQ51_12190"
     CDS             complement(3662..5800)
                     /locus_tag="VGQ51_12190"
                     /inference="COORDINATES: protein motif:HMM:NF037085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HEV9037381.1"
                     /translation="MHKKTLLLTAMLIASCPLLFGQPGNGNGNGDKGPSATLVFSPAT
                     MPDGRYGSSYKNLTLDVSGGKAPYSFSLSGGALPPGMSLSANGVFSGAPQAAGSYSFT
                     VSVTDHSKNPLSGSQKYSLVIDPAVAQVTANAATKTAGAPDPVFTYAVGGLLNGDNSG
                     ILKGNLSRTPGESVGIYPISKGNLSAGGNYTIAYTGNFFTIVQGSGSGNGSGTEGNPP
                     PGNLVFSTTSVPDGQYGSSYGNSTLKVSGGSSPYSFSVAAGDLPPGISLSTGGVFSGV
                     PKTAGSYSFTVSVTDHSKTPLSASQNYTLIIDPAALTVSANNTGMTYGGTVPSLTVSY
                     NGLVNGDKASGLTTPAIATTTATSSSATGAYSILASGAADPNYSFTYKPGTLTISPAT
                     LTVSANAVTKVFGAPDPSLTYTANGFANGDGTNLFTGNLSRSPGENVGKYSISRGSLS
                     AGGNYSIGFTGNYLTITKASQQITWTQNLLVGCQSASQLQLNATASSGLPVSYSVSDA
                     SVASVSGNILTLLQPGTTVITANQGGNSEYPPATAVSDTLVYQPVSLIDQHWSDVLFF
                     DNSSGDFAGWQWYKNGQAVPGATDPFYSDTPALNGQYYVIATNKGGQKIESCTMTITG
                     GAAIQGGIKVFPNPANAGGLITITSNYSNPALKGAVLQVVDVNGKIRQQLTVVKSTSQ
                     VTMPSDAGIYIVNLLLAGGQKASTNVLVIN"
     gene            complement(6143..7399)
                     /locus_tag="VGQ51_12195"
     CDS             complement(6143..7399)
                     /locus_tag="VGQ51_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037382.1"
                     /translation="MKNLLILLVAMLGWSVSRADIKFVNFNKIDPSGRYAGRFSQLVE
                     HLPYYDHFSPDWIYEIPKDTLVQELTAGLRLFGTLKGDELETDLLLGELAHYLYNLDQ
                     KTYYDTAELYLTKAIGLDGKDPRGYWFLAYHYALSNEVVKGVQTFEQARSLVSDATPG
                     EFWQEYAFAMMLGSMPSHCRYALDQYKHKGGTSQLSIVMDSVMRSKTIPADADSAYTA
                     RDLWEGFPSGARIAMISRALGVSLEVDTTDSLQMNEFSERKTAMVMQSTLIRGIRGKS
                     FGYNIAMIVKVAEKGEDLGIFMTSIVPPGAKRDSVFLFSSLYPHGISYTFRDNRVYAD
                     RGGAHIHFIAIERESPVYPGLFLENHASEIRGTPGRLEFYTLGAVRSRFPGRIFYFFV
                     LDTCGDIYKRSWDDFSRLITNGLILD"
BASE COUNT         1710 a         2082 c         2056 g         1698 t
ORIGIN      
        1 gcaggcttcc tgccgggtca attggacctg gcctgggtct atatctatac cttacgtaag
       61 ctgagaatgg tggcctggta cagggattcg aaacaagaat tcctaaaaaa gtacgcaacg
      121 gactcatatt caataactaa cgcccaaatt cccgcccccc tccaggactt ttaaaaccaa
      181 aaaaatagta gtatcttgtt ggatatccaa taagtatgaa aaacgacgcc cgtttcgcgg
      241 tgttcttcat aatcctcacc ctccattggg acatcgcctg cggccagaca ctttccggca
      301 cggtcaattc ctattactcc attacagcgg tcaacagcgc ggccaatgcg gtgacggtcg
      361 ataatgcctc cggtctctcc gtgggtcagc gtgtattcct ctatcaggcc aaaggcgcct
      421 cgatcaccgc aaccaataca gccaactatg gcgatattac cgcggcaaat aatgccggag
      481 catatgaatt caatatcatc tgcacgatca gcggcaacga gatctggatg ctgaatgccc
      541 tggcgaatac ttatgatccg acagctatgg tacagctggt cacggtgcca tacagttcgt
      601 cgctgacggt tggcggcacc atcactcctc aaccctggga ttctctcacc ggaaagggcg
      661 gtatcgtcgt gctggaagct accggctcca tcacgctgaa cgcggacatc aatgtcaccg
      721 gtcagggttt cgaaggaggc acattgatca actatgctgt ccctccctac aactgcagct
      781 gggccgttac ggtcagcgct tactatctgt cctatccggc cagcgggttc aacaccggcg
      841 gaagaaaggg agagggcatt gcggcttata ttaccggcga agaatttggc cgcggcaagt
      901 tggccaacgg aggcggtggc ggcgacaatg gtaactcggg cggtggcggc ggcggcaatt
      961 atggtacggg aggcgcgggt ggacaacgca ccggcgaatc gaccttcgat tgtcatgccc
     1021 aatatcccgg tgtcggaggc gccagtgttg cagcttatgg ctattcgtcc gcgaaaaacc
     1081 gcatcttttt tggcggcggc ggcggcagcg gtgaagaaaa taacggctat ggagaacccg
     1141 gcgggaatgg cggcggtatc atcgtgttgt cggctccagt gatcgttggc ggaggaggac
     1201 ggctattggc gaccggtgtc tcacctacga atcccatctg taccgatcca tatcaggccg
     1261 aaggcgatgg cggaggcgga ggcggcgccg gaggaacgat cgtcctggat gcgtcaacca
     1321 ttaccgggac catcacggct gacgccagcg gcgccaacgg ctcgaattcc agcaatcacg
     1381 tgggtgactg taccggtccc ggcggaggtg gagcaggcgg aatggtctgg acggcaggat
     1441 cttctttccc gggtgcggtc accgccacgg tcagcggcgg agccaacggt gtcgtcagtt
     1501 ccggcagcac caaaagccct tcctgtaagg ggtttgccaa tggcgccact gcaggaacgg
     1561 ccggaatgac actcagcagc tttgcgcccc cggtctcttc ggggccggta tgcgttgccc
     1621 tggcctcacc cgtcttacaa tatttcaatg ctatgcgcac cagccaggat atccagctgt
     1681 cctgggcatt ggtgtcccct gaaacggcca cggatatgcg aaacttcatt cttcagcgat
     1741 cctccgatct gtcgcatttt gccggcgtcg caaccgtttc ctgctcgaaa gattcgatca
     1801 gctatcatta caccgacagg gcggcgaata gtaacgggac tctggattac cggctggcct
     1861 ggcagaattc aaacggggaa tggttctatt cacgcgtcgt cgccgtcgcg ggagtgccgg
     1921 gtcccgatgg aagctctatc cgtctttatc ccgaccctgc gaccaattac ctgaccctga
     1981 ctgtgatctc ctcggccaac gaaagtgcta ccgtatcgat ctcgagcgca ctggggcagt
     2041 cgctgctcac tcgaccgatc tcgctgttca agggcctgaa tacggtgacc gtccctatcg
     2101 caacactggc accagcggca tatttcctcg tcctccggtc ggggggaaac aggatagtga
     2161 aaccttttct caaaaagaac caatgatgtg aatggcaacc cgacggttcc tataattggc
     2221 agccggatcg taagacaaga ccgggtcgct ctttttcaga tagcctatat ccatgcggcg
     2281 acggctgatc cctttgttct cgagatattt tacgacggct ttcatacggg tttcggcagt
     2341 ttgactattt tccatctgca tcaggctatc gcaaaaatga ccctcgatgg aaagccggag
     2401 atcgggatga tctttcaata agctgaccaa ttcatccagt tttgctttct gcatatcggg
     2461 aatggcggtt tcaccgatga tgccaaagac cacattccgg cggctcctct ctgcctggct
     2521 gcgctttgcg gactgcgcct catccctccg gatcaacgaa tccagggaga tgttatccgt
     2581 tgaaacagcc gtcgcaccgg cttcagcgac cgtcgaatct ttcgcgggcg gaagcgatgc
     2641 ctgtttagga gtcacctgga ccgccgaatc tttcgcgggc ggaagcgatg ccgggacacg
     2701 cgacaccggc tcagtagcac ggggcggcga atttttcgca ggagacactg ccgccgaagc
     2761 ctgccgcagc gaatccgttt catgcctttg tgtccttttg cgccgtcccg gggacgttgc
     2821 cgatccgaaa ctcagcctga cctgtacacc gaaggagagc aaggttgcag cgccggaatt
     2881 tggcagatgc atcgctccat tggcctttac atggctgatg ccggcagtac tgtacgtgac
     2941 aagcggcatg ctgtcgcttt tctctctgag atcggtgagc ccataatcga catatacgcc
     3001 ggtgtagatc cgcgtgtggc gggaaatagc gaaactaatc cctgttcccg cacttaatgc
     3061 cccggcgggt ttcaacccgg tggtcgagcc ggcatgccat ccattcacag taccaaaacc
     3121 atgctggggc agattggaaa caacgatatg aaaattggga tagtagccgg cgagactcaa
     3181 ttgacgtgcc gaagcctgta tgctggtatt gaaagggaaa atgaatttgc cgccgccgtc
     3241 gaaataccat tccaggcctg cgcctccggt atgaaaatgc aacagcaacg gtatggtcgc
     3301 ggcatagaat tgttgtgttt ccttgtatcc cgtcgccttt atattgtagt tgaaagcaga
     3361 acccccgtcg tctacctggc cagagctata ggtgccgtcc ttcagactag cctgtgtcct
     3421 gtagatcgag cccaggatac cggtacgaac accccattgg ctgtttaccc tgaatgtata
     3481 ggtcagaccc aaagaaccgc cgggcaatgc cgtagaatgg ccgccgggta atttgtattg
     3541 catcccctgc aaaccgccat ccagctctat acccaaaccc tgtgcgtcgc ttttggcgct
     3601 gattgcggcg agactcaccg ccaatatgac tatttttctg aatgttctca ttggtaatag
     3661 gctaattgat caccagcaca ttggtgctcg ctttttgacc gccggcaagc agcagattga
     3721 cgatatagat accggcatcc gaaggcatgg tcacctggct cgtcgacttg acgaccgtca
     3781 attgttgccg gatcttaccg ttgacatcta ccacctgcag gaccgcaccc ttcaaggcgg
     3841 gattagaata attactggtg atggttataa gtccgcctgc gttggcggga ttgggaaaca
     3901 cctttattcc gccttgaatt gcggcgccgc cggtaatcgt cattgtgcag ctttcgattt
     3961 tctggccgcc tttattggtc gctatgacat aatattgtcc attcaaagcg ggcgtgtcgc
     4021 tataaaatgg atcggtcgca ccgggaacgg cctgcccgtt cttataccat tgccatcctg
     4081 caaaatcgcc gctgctgtta tcgaaaaaaa gcacatcgct ccagtgttgg tcgatcaggg
     4141 aaaccggctg ataaaccagc gtatcgctaa cggccgtcgc gggcggatat tcggaattgc
     4201 ctccctggtt cgcggtaatg accgttgtgc ccggttgcag caaggtcagg atatttcccg
     4261 atacacttgc tacgctcgcg tcggataccg aatagctcac cggaaggccg ctgctggcgg
     4321 tcgcattcaa ttgcagctgt gaggcagact ggcatccgac caacaggttc tgcgtccagg
     4381 ttatttgttg agaagccttt gtgatagtta agtaattacc ggtaaagccg atcgagtaat
     4441 tgccgcccgc gctgaggctg cctctcgata tggaatattt gcccacattc tcgcccggac
     4501 tcctgctcag gttgccggta aacaggttcg ttccgtctcc gtttgcaaag ccattggcag
     4561 tgtaggtcag cgatgggtcc ggagcaccga atacttttgt caccgcattg gccgatacgg
     4621 tcagcgtggc ggggctgatg gttagcgttc cgggtttgta ggtgaagctg taattcggat
     4681 ctgccgcgcc ggatgccaag atggaatagg cccctgtcgc ggaagatgag gtagccgtag
     4741 tagtggcgat agccggcgtc gtaaggccgg aagctttgtc gccattgaca agtccgttat
     4801 aagacaccgt cagcgaaggc accgttcctc cgtaggtcat gcccgtattg ttggcggaga
     4861 ccgtcagtgc ggcgggatcg ataatgaggg tataattctg cgaggccgaa agcggagtct
     4921 tggagtgatc cgtgacgctg acggtgaagg aatagctgcc ggccgtctta ggcacccccg
     4981 aaaaaacgcc gccggtagac agcgatatgc ccggtggcag atctcctgcc gccaccgaaa
     5041 aggagtatgg actgctacca cccgagacct tcagggtcga attcccgtat gacgacccgt
     5101 attggccgtc ggggaccgat gtggtggaaa ataccagatt ccccggtggc ggattacctt
     5161 cagtgccgct accattgccg ctaccactac cctgaacgat ggtaaaaaaa ttgccggtat
     5221 aggcgatagt ataattgccg ccggcgctga gattaccttt tgaaatagga taaatgccca
     5281 cactctcccc tggcgtcctg ctaagattgc ctttaagtat ccccgaattg tctccattca
     5341 gcaaaccgcc gaccgcatat gtaaaaaccg ggtccggagc gccagcggtc ttcgttgcag
     5401 catttgctgt tacctgcgct acggccggat cgatgaccag gctatacttc tgtgagccgg
     5461 aaagcggatt tttggaatgg tccgtgacgc tgacagtaaa ggaataactt ccggcggcct
     5521 ggggcgcgcc ggaaaaaacg ccattggcgg ataaagacat gcccggtggc aacgccccgc
     5581 ccgatagcga gaaggaataa ggagccttgc cgccggagac atccagcgtc aaatttttgt
     5641 aagacgaccc gtatcggccg tcaggcatcg tagcggggga aaatacgaga gtggcggatg
     5701 ggcctttatc accattgcca ttaccattgc cgggttgccc aaacagcaaa ggacacgatg
     5761 ctatcagcat ggcagtaagg agcagtgtct ttttgtgcat acaggaaaac agatagtttc
     5821 gaccacggtt taaggtcagg tatttctgtc aaaatgtatg cgctgcaatt cccatgcccc
     5881 ggcgcagccg gctgcagctg ccgaaagccc cccagctgtt ccgaagggag accacattca
     5941 actgctgcag gctgtgctgg cttgagtcgg actgacgtct aaagataaac atcttcggcc
     6001 gaagccagac agccgcttgc cagtttatgt ccttaatttt tgggcattgg ttgaagaaaa
     6061 atatccgttc gtccgtccgc ggtttgatcc ggaagccgtc cgtccgccat tggatccgga
     6121 agccgtttga tccggaagcc gttcaatcca gaataagccc gttggtgatc agccggctga
     6181 aatcatccca tgatctttta tagatgtccc cacaggtgtc cagaacgaag aaatagaaga
     6241 tcctgcccgg aaaccgagag cggactgctc ccagcgtata gaactccaac cgcccgggag
     6301 tcccgcggat ctcagaggca tggttctcca ggaacagacc cggatagacg ggcgactccc
     6361 gctcgatggc gataaaatgg atatgggctc ctcccctgtc ggcataaacc ctgttatccc
     6421 gaaatgtata ggatatgccg tgcggataca acgaagaaaa gagaaatacg ctgtcccgtt
     6481 tggcgcccgg cggcacgatc gaggtcataa aaatgcccag atcctcccct ttctccgcca
     6541 cctttacgat catcgctata ttgtatccga atgacttacc ccggataccg cggatcaggg
     6601 ttgattgcat gaccatagcc gtttttcgtt cgctgaattc gttcatttgg agggaatcgg
     6661 tcgtgtccac ctccaggctg acaccgagcg cgcgcgagat catggctatt cgtgcgccgg
     6721 atggaaaacc ttcccataga tcgcgggcgg tataagcgct gtcggcatcg gcagggatcg
     6781 ttttcgaacg catcaccgaa tccatcacta tagacagctg gctggtccca cccttatgtt
     6841 tgtactgatc cagcgcatac cggcaatgcg atggcatact gcccagcatc atggcaaacg
     6901 catattcctg ccagaactca cccggtgtgg catccgatac cagacttcgt gcctgttcga
     6961 acgtttgaac ccccttgacc acttcgttgg acaaggcata gtgataggcc aggaaccagt
     7021 acccgcgggg gtcctttccg tcgagaccta tggccttcgt cagatagagc tctgccgtgt
     7081 cataataggt cttctgatcg agattataca gataatgcgc cagctcccct aacagcaggt
     7141 cggtttcgag ctcgtcgccc ttcagggtgc cgaacagccg cagaccggcg gtcagctcct
     7201 gaaccagcgt atccttcggg atctcataga tccaatcggg ggaaaaatga tcgtaatacg
     7261 ggagatgctc caccagctga ctgaaccggc cggcataccg acccgaagga tcgatcttat
     7321 tgaaattcac gaatttgata tctgcccgag acacggacca gcccagcatc gccaccagga
     7381 ggatgaggag atttttcata ggcgaacaag atacattttt ttcgttcgtc tgtgtaacca
     7441 ttttaccttt ccggagtaat ctatacggaa cacggacgac cacggtccat tggccgcaaa
     7501 caataaaaga aatcccggtg ccggaattat ctgacgagaa aataat
//
LOCUS       DASYSV010000398        15310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1104303, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000398 DASYSV010000000
VERSION     DASYSV010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 15310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15310
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104303"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VGQ51_12200"
     CDS             complement(<1..526)
                     /locus_tag="VGQ51_12200"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9037383.1"
                     /translation="MKRILFNRTLLGLALAVVVLGGCSKVLQEQPRATYTPSFFTTQT
                     GVIGGLTEMYAHLRNMYGQGYWYNTLEVGTDESTYGQSADGNFLVMDMTGQGAITSTT
                     SRSDVIWNNCFTNINTASGVIQYGAKAGVSIALTSEANFFRAFDYFLLVQTFGGVPLD
                     LGAGTLQFNTAPTPT"
     gene            complement(538..3651)
                     /locus_tag="VGQ51_12205"
     CDS             complement(538..3651)
                     /locus_tag="VGQ51_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9037384.1"
                     /translation="MRKFKVRNTLVSSILMTAVSLLLSSLLYAQDVGAPVHGNVTDSS
                     GKPVSGASVTNLKTKKGTSTDQSGNFTIGAVNGQTLEISYVGFQKARVVYRGQSNISV
                     SLVAESAELTNVVVIGYGTQRKEAVTGSVASITGSNLREVPSPNITDAMQGRLPGVAI
                     SQTSSQPGASMQIRIRGTRSLTATNDPLIVLDGIPFPGNIADIDMNDVKSVDVLKDAS
                     ATAIYGSRGANGVVLITTYKGTSGQEPRITVNGYYGVNKIFSKFPMMNGPQLLALRQT
                     AGRYLQQGGAAQLGTDETTTNNTDWQNLFYQNGKVTNEDISVSSSTRGGSYNFGAAFY
                     DNIGVIPTQSYRRMTIHGSLDQRVGKYVRMGFTTQDNYSYTQGAQVGLYNVLSNSPLA
                     SPYNANGSIKYIINMPLDQQWVETKKVVDSLKNQYLQQTKGYSSYNSVYGEVHVPGVE
                     GLAYRINIGLNYSTGSTGGYTAVGVNSNNPTAPSTASLNYSTNTDWTIENLLTYDRTF
                     NKSQINALALYSTEKTQYNFSSLSGIGIPADGLQYYNIGTASQEIDVLPGVPQNQNYY
                     QRGLLSWMGRLMYTYDNRYMISATVRSDGASVLAPGHQWHTYPAVSAGWNIANESFMK
                     GVPSINMLKLRVGYGETSNQAVNPYSTLGLLSTNPYNFGSTYATGYYVSTLPNPQLSW
                     EFSKTWNYGVDFAALNNRLSGTVEYYVTNTNGLLQQVTLPPTSGVGSYTGNVGNTQNK
                     GIEFSLDGTILKTHSGVTWDLGVNMSFNRNKVTHLASGVLADQANWWFVGHPINVIYD
                     YQKMGLWKDAADSAAGHENILQPGGTLGTIKVKYTGGYGANGVPNRAIDPDDRQVLNA
                     DPTFIGGFNTRVSYKGFDFSAVGIFQDGGILISTLYGSASYLNLESGRRNNIKINYWT
                     PTNTNAKYPNPAGQISGDNPVYGSTLSYFSGSYLKISTLSLGYNFARFAKRFGIPSAR
                     IYVTAQNPFVTLSPYHKETGLDPETNSYGDQFAAVPLSSSLHRLLTQGFNAPATRSYL
                     VGLNLTF"
     gene            3944..4984
                     /locus_tag="VGQ51_12210"
     CDS             3944..4984
                     /locus_tag="VGQ51_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV9037385.1"
                     /translation="MTEFKEVTIYDLARKLNISIATVSRALKDDPVVSKKTKKKIADL
                     AQELGYRSNHFARNLRTQRTNTIGVIIPRINSYFMSSVIAGMENIATREGYNLIISQS
                     SESVQKEITNANTLFNSRVDGLLVSLAYDTDSLAHFEPFVKKKTPIIFFDRTAEQSNG
                     TCVLINNMKAAYEATTHLISQGRRRIVHITATPKRNVYVDRLKGYKQALADQRIVFRE
                     DLVIVGNLSQEAGAEAAAVIREMRPLPDAVFVANDNCAVGCMTALKQTGIRIPEDIAF
                     VGFNNDPVSTVVEPNLTTVNYPGYEMGQIAARHLIDHLAGSSNIQSTNTIILRSDLII
                     RESSLTRKPNAS"
     gene            4981..7107
                     /locus_tag="VGQ51_12215"
     CDS             4981..7107
                     /locus_tag="VGQ51_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HEV9037386.1"
                     /translation="MKRFACILFSLLLLRSSRVLAEDGYRLWLRYDRIDDRQLLQQYL
                     RTIPSCTFPATGSEILPAARNELTNAIAGLLGHPPDYTTIPSANSLLIGTPASLPFLT
                     RLHWDADIHRLGDEGFIIRTYTLDNRSGIAIVAASDKGVLYGVFQFIRLLQTHQPLTH
                     LDIISIPTRAYRILDHWDNMSRNVERGYAGLSLWDWFTLPTYKDQRYTDYARANASIG
                     INGAVLNNVNANYLFLTPAFLEKAAALADIFRPYGIRVYLSVNFASPIQLGGLHTADP
                     LDPVVREWWKKKADEIYRYIPDFGGFLVKANSEGQPGPQNYGRSHADGANMMGEALAP
                     HHGIVIWRAFVYNATAADRARQAYDEFHSLDGKFDSNVLVQIKNGPLDFQPREPFHPL
                     FGAMPDTRLMLEVQITQEYLGASNHLVYLAPLFAECMDADTYTHGNGPTVGQSISGMA
                     GVANTGNDINWCGHIFAQANWYAFGRLAWGATPAGIADEWIRQTFTNNTTAVDSIRRM
                     MLASREITVDYMNPVGLNMIFAEGHHYGPGPWYDKAKRPDWNSTYYHHADSAGIGFDR
                     TPTGSNMLEQYAPGARAEWEDVKTCPDKWLLWFHHVPWTWKMHSGRTLWDEMCYRYQK
                     GVDSVRWMQRCWQRLRPDIDQERWRQVDMKLRIQEHDAVWWRNACLLYFQTFSRMPIP
                     AGVEPPDHTLEYYEDIQLHDVPGTGR"
     gene            7123..7869
                     /locus_tag="VGQ51_12220"
     CDS             7123..7869
                     /locus_tag="VGQ51_12220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV9037387.1"
                     /translation="MDTSQKIAIITGGGSGIGLAITKKFVENGILTIIVGRHRDKLDA
                     AKKQLGDLCHPMPQDLNDLNAIPGLVRRIIDHYDHIDILVNNAGINMKKELPDVTDED
                     FERILLTNVRSVFTLTREVVKTMLPHGKGNIINISSMASQYGIPKVIAYTASKSAIEG
                     MTRAMAVELSPKGILVNCIAPGFIATDMSAKALDTDAERKQKVFSRTPMGRMGDPADI
                     GDAALFLASNQAKYITGVVLPVDGGNSIGF"
     gene            7881..11657
                     /locus_tag="VGQ51_12225"
     CDS             7881..11657
                     /locus_tag="VGQ51_12225"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HEV9037388.1"
                     /translation="MKRISIVLLLTLSIGTAGAQSFPIVTKNEGATQLFVDPNDHWLV
                     NRTAQLLQEDIERVTGIKPVLLHALPDHPVDNLIVIGSMDSSAIVKQLLRRHRLPKLA
                     GKWEAYSIRTLANPTPTIAHALVIAGSDRRGTAYAALEISREIGVSPWYYWADVPTAH
                     KDELYFTRDSILFNGPAVKYRGFFINDEAPALSGWVKATFGNFNHVFYEKVFELLLRL
                     RGNYLWPAMWGNAFNDDDTLNPVLASEYGIVMGTSHHEPMLRAQQEWKRYGHGPWDYT
                     VNAPTLDSFWEKGIRHMDHHESIVTVGMRGDGDKPMTEGSNIALLEKIVADQRAIIQK
                     VTSQPASQTPQSWALYKEVQDYYDKGMRVPDDVTLLLCDDNWGDVRKLPTLGSKPRQG
                     GYGMYYHFDYVGGPRNYKWINTNQLSRVWEEMHLTREYGDDKIWIVNVGDIKPMEFPT
                     QFFLDYAWDPAALPADRLPEYTWHWAAEQFGASHAAAIADILSKYTKYNARRKPELLS
                     PDTYSLPNYHEAETVADDYKTLAQRAEYIGRMLPAQYHDAYYELVLYPVLASANLNEL
                     YYTVARNRLYATQGRALTNRLAAVADSLFGRDSLLSLTFNHDIAGGKWPHMMDQTHIG
                     YTYWQEPRHNNKPKTDTIDLAAATGPSWGVFPTTLSGFNPYDFQSQYIEVFSRRSAPT
                     PCTVTPDAPWIDAHTTGNPTSLQQRWQVSIDWAKVPTGQHQGSLTITGPDGTPLTVTI
                     NLNNPAPAPPLDFKGFIETNGCVAIEANNFTKAVTTPAINWTVIPDLGRTGSAVEASP
                     VTAPTQTPGPHSPCLEYPVWLFDTGTVAVQAYCSPIIAFNGKPIRYAVGIDEETPQVI
                     DLTTGNEARGTWDKMVSDNIKVSISHHAVSRPGAHILKFYLVDPGVVLQRLVIDAGGV
                     KPSYLGPPQSPRRGLKDIYHDFFPIGVAVSPRSLAGPDSTLILQQFNSLTPENAMKMG
                     PIHPEENRYNWKDADAIVDFAQRHGLLVRGHNLCWHQQTPPWMFKDTSGGAVTKEVLL
                     QRLKDHINTVVSRYRGKVYAWDVVNEAIDDDSTKFLRNSEWYRICGEDFIVKAFEYAH
                     AADPSAQLFYNDYNTERPEKRERVYRLLKQLVDAGVPITGVGLQAHWSVNEPSAHDLQ
                     TAIDRFSSLGLKVQVTELDISIYPWEKFRRTRRPGEQDVYTPEMEKRQAAQYKMAFDI
                     FRAHRASINGITFWNISDRNTWLDNYPVTGRKNYPLLFDINNQPKKAWWEVVDF"
     gene            11772..13928
                     /locus_tag="VGQ51_12230"
     CDS             11772..13928
                     /locus_tag="VGQ51_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV9037389.1"
                     /translation="MHHRFLSLFAALLLFAAATGQTLPFRNSSLPTADRVTDLLSRLT
                     LQEKVSLLGYRSKSVDRLSIPAYNWWNEGLHGVARAGEATVFPQAIGMAASFDAPLLR
                     KLADAVSTEARAKYNLSTGLGRHEQYMGLTFWSPNINIFRDPRWGRGQETYGEDPFLT
                     ATMGSAYVKGLQGNDSRYLKTSACAKHFAVHSGPESIRHTFNAIIDEKDLRETYLYAF
                     KRLVDSGVESVMCAYNRVNGEPCCSGPTLLHRILREEWHFSGHVVTDCGALDDIFERH
                     KALPGPVETAAAAIKAGVDLDCSDILQDDVLKAVDKGLLTRRDVDSALAPLLRTQLKL
                     GFFDDKTDIPFSGYGSDSVHNDYHIALARQMAAESIVLLKNENNVLPLRKDSLASLMV
                     VGPNAFSFDALAGNYHGVSSRVVTFLEGITAATGPAVHVEYDQGCDYKDSIHFGGTWA
                     ASNCQTTVAIIGLTPVMEGEEGDAFLAPHGGDRTDLALPAAHIAYIKALRKAIGHKPL
                     IVVVTAGSAVDLSPLEPYADAILLAWYPGEQGGNALADILFGKISPSGRLPVTFYRST
                     ADLPSYKDYSMKGRTYRYYDGPVQYPFGFGLSYTSFAYHWQTTPKKTYTPADTLSFSV
                     TVDNTANRDGDEVVQAYIVYPDLERMPLKELKAIGRVSIPAHDARRLTFSIPLQQLHK
                     WDLAAHAWKVYPGNYKLVLGSDSRNEKLVAEFTLHR"
     gene            complement(13917..15110)
                     /locus_tag="VGQ51_12235"
     CDS             complement(13917..15110)
                     /locus_tag="VGQ51_12235"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEV9037390.1"
                     /translation="MKGATRRLASIDAFRAITMFFMIFVNDLVFDFRVPDWLEHAREG
                     EDRLGFADTIFPAFLFIVGCSIPLAIRHGHEKGFTRWDTLRHILRRSLALVIMGYFLV
                     NYEEFTTGPAWISKFGWLLVVTLAFFFIWLVYPKEWPWWRKGVFRTIGVAALALMAFV
                     YKGGTPEAPKGMNDYWWGILGLIGWAYLTCALLYLLIGERLGVLGLVFLYFLGFSIAV
                     CSGWGWIRPAVSWLTLGDSGGLSAFTMAGVMTTVYYRRMSEKGLAMKSIYRLLAWVPL
                     LVATGFWLRPLKGISKLEVSPSWILICTGISVACAAGLAFIVDVRGKQNWYRWIRPAG
                     TITLTCYLLPDIHFAIIRLLGSAWQLPVVLRTGATGVIKSLVFAFLIVLLTGVLEKKK
                     IRLSV"
     gene            complement(15122..>15310)
                     /locus_tag="VGQ51_12240"
     CDS             complement(15122..>15310)
                     /locus_tag="VGQ51_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HEV9037391.1"
                     /translation="ILEYTEEPLVSLDIKGNTNSCVVDGTLTSAVGRLVKVIAWFDNE
                     YGYTSRMIDWLSYLRKFL"
BASE COUNT         3623 a         4867 c         3653 g         3167 t
ORIGIN      
        1 aagtcggcgt cggcgccgtg ttgaattgaa gcgtacccgc gccgaggtcc agcggcacgc
       61 cgccgaatgt ctgcaccaat aagaaataat cgaatgcccg gaagaaattc gcttccgacg
      121 tcagcgcgat gctgacaccc gccttcgcgc catattgaat gacaccgctg gccgtattga
      181 tgttggtgaa acagttgttc cagatcacgt cgctacggct ggtagtagag gtgatggcgc
      241 cctgacccgt catgtccatg accaggaagt tcccgtccgc gctctgacca taggtcgact
      301 catccgtgcc gacttccagc gtattatacc aatagccctg tccgtacatg ttccgcagat
      361 gggcatacat ctccgtcaat ccgccgatga cccccgtctg ggtggtgaaa aaactgggtg
      421 tataagtggc ccgcggctgt tcctgcagga ccttgctgca tccacccagg accactaccg
      481 ccagggccaa tccaagcaat gttctattga aaagtatccg tttcatttta acaagattta
      541 aaaggttaag ttaagaccga ctaaatagct gcgtgtggca ggcgcattga atccttgcgt
      601 cagcagacga tgcagagacg aagacaacgg taccgcagcg aactgatcac cataagagtt
      661 cgtctccgga tccaaaccgg tctctttatg gtacggcgac aaggtcacga acggattctg
      721 cgccgtaacg tagatccggg cggacggtat gccgaagcgc ttcgcaaacc tggcaaaatt
      781 gtatcccagc gaaagcgtgc tgatctttaa gtacgacccg ctgaaatagc ttaaagtaga
      841 accgtataca ggattgtcgc cgctgatctg gccggccggg ttgggatatt tcgcattcgt
      901 gttggtcggc gtccagtagt tgatcttgat gttattccta cgtccgctct ccaggttgag
      961 atagctggcc gatccgtaca aggtgctgat caggatgccc ccgtcctgga aaatgcccac
     1021 cgccgagaag tcgaaccctt tgtaactgac ccgcgtattg aagcccccga tgaaagtcgg
     1081 gtctgcattc agcacctggc ggtcgtcggg atcgatggcc ctgttcggta ctccgttcgc
     1141 gccatagccg ccggtatatt tgaccttgat cgtccccaaa gtcccgccgg gctgtaagat
     1201 attttcatgg cccgcagcgc tatcggccgc atccttccac aatcccattt tctgataatc
     1261 ataaatgaca ttgatcggat gaccgacaaa ccaccaattc gcctgatccg ccaatacgcc
     1321 ggatgccaga tgggtgacct tgtttcggtt gaaagacata ttgacgccga gatcccaggt
     1381 cacaccgcta tgggtcttca ggatggtccc atccagcgaa aactcgatcc ctttattttg
     1441 tgtattaccc acattgccgg tatagcttcc cacgccggaa gtcggaggca gcgtcacctg
     1501 ctgcaaaaga ccgttggtgt tggtgacata gtattcgacc gtacctgaca accggttgtt
     1561 cagcgcggcg aagtcgacgc cgtagttcca ggtcttcgaa aattcccagc tcaactgcgg
     1621 gttaggcaac gtagagacat aataacccgt cgcataggtg gagccgaaat tgtacgggtt
     1681 ggtgctcagg agacccaatg tggagtaagg attcaccgcc tggttggaag tttccccata
     1741 accgacacgc agtttaagca tattgatcga gggaacacct ttcatgaaag attcgttggc
     1801 tatattccaa cccgcgctga cggcgggata ggtatgccat tgatgcccgg gagcgagtac
     1861 cgatgcacca tccgacctca cagtagctga tatcatataa cggttgtcgt acgtatacat
     1921 caagcgcccc atccaggaca acaatccccg ttggtagtag ttctggtttt gcggaacacc
     1981 gggcaaaaca tcgatctcct ggctggcggt gccaatattg tagtattgca gcccatcggc
     2041 aggaataccg atcccggata aggaactaaa attgtactgg gtcttttcgg tcgaatacag
     2101 tgccagtgca ttgatctgac ttttattgaa cgtccggtca tacgtcagca ggttctcgat
     2161 cgtccagtcc gtatttgtcg aatagttcag ggacgcggtg gaaggagcag tcgggttgtt
     2221 gctgtttacg ccgacggccg tataacctcc cgtactgccg gtgctgtaat tcaaaccgat
     2281 gttgatccgg taggccagac cttccacacc cggcacgtgg acctcgccat aaaccgagtt
     2341 atagctggaa tatcccttgg tctgctggag atattggttc ttcaggctgt cgaccacctt
     2401 tttagtttcc acccactgct gatccaaagg catgtttatg atatatttaa tggatccgtt
     2461 tgcattatac gggctggcga gcggtgaatt ggataatacg ttatagagcc ctacctgcgc
     2521 tccctgcgtg taactgtaat tgtcctgggt ggtgaagccc atgcggacat atttaccgac
     2581 gcgttgatcc aacgaaccat gtatcgtcat tcgccggtaa gactgggtag gtatcacgcc
     2641 tatgttgtca taaaaagcag ccccgaagtt gtagctcccg cccctggtcg acgaggaaac
     2701 gctgatatcc tcgttggtca ccttaccgtt ctggtagaat aggttctgcc agtcggtgtt
     2761 gttggtggtg gtctcatcgg tccccaattg ggcggcgcca ccctgctgga gataacggcc
     2821 cgcggtctga cgcagcgcca gcagttgcgg tccgttcatc atcgggaatt tcgagaagat
     2881 cttattcacg ccataatatc cgttgaccgt tatccgtggc tcctggcccg atgtaccttt
     2941 ataagtagtg atcaggacta ccccgttggc cccgcgcgaa ccgtaaatgg ccgtcgccga
     3001 agcatccttc agcacgtcca cgcttttcac gtcattcatg tcgatatcgg cgatattccc
     3061 cgggaaaggg ataccgtcca gcacgatcag cggatcattg gttgccgtca gcgaacgcgt
     3121 accccggata cggatctgca tcgaggcgcc cggttgcgac gaagtctgtg agatggccac
     3181 acccggcaaa cgcccctgca tggcgtccgt aatattgggg gaaggcacct cgcgcaaatt
     3241 gctaccggtt atcgaagcga ccgatccggt taccgcttcc tttctttgag tgccataacc
     3301 gatgaccact acattcgtaa gctcggcgct ctcggctacc agcgatacgg agatgttgct
     3361 ctgtcccctg taaacgaccc ttgccttctg aaacccgaca taggatatct ccagggtctg
     3421 accgtttacc gcaccgatcg tgaaattacc gctctggtcg gtggaggtcc cttttttggt
     3481 cttcaggttc gtcacggaag ccccgctgac aggctttccg ctcgaatccg ttacattccc
     3541 atgcaccggc gcgcctacgt cctgtgcata taataatgat gacaagagca agctcacagc
     3601 agtcatcagt atgctcgaaa caagggtgtt tcgtactttg aattttcgca tatggagttt
     3661 agttttttta agaagtaatg aaggaaatgc tacaatgtgg atggaggtgg caggcgatcg
     3721 cctggatcac tacggagaat aatagccgat aagtaaaggc aatgccgctt cgggagaaag
     3781 gtccctatcc gggaaatcat atggctgcat aagatcgatc gttgttttgg tctgtgattt
     3841 ggcaaggcta aaatagggaa aattccgaca aaatgtaacc gattacaatt tttttgaaaa
     3901 aaaaccctat ttttacttgc accttccgca cgtaaacgat tgtatgaccg aattcaaaga
     3961 agtgaccatt tacgacctcg cccgtaaact gaatatctca atagccacgg tcagcagggc
     4021 tttgaaggat gaccccgtag tcagcaagaa aaccaaaaaa aagatagccg acctggccca
     4081 ggaattaggt taccgatcga atcatttcgc ccgcaatctc cgtacccagc gcaccaatac
     4141 catcggcgtg atcatccccc ggatcaatag ctatttcatg tcctccgtca tcgcaggcat
     4201 ggaaaatatc gcaacccgcg aaggctacaa cctgatcatc agccagtcgt cggagtccgt
     4261 ccaaaaggag atcaccaacg ccaacacctt atttaatagc cgcgtcgacg gcctgctcgt
     4321 ttcattggcc tatgatacgg actccctcgc ccacttcgag ccctttgtta aaaaaaagac
     4381 cccgatcatc tttttcgacc gtaccgcaga acagtccaac ggcacctgcg tccttattaa
     4441 taatatgaag gcggcctacg aggccaccac ccacctcatc agccagggcc gccgccgcat
     4501 cgtccatatt accgccaccc ccaaaaggaa cgtatacgtc gaccgcctca aaggatataa
     4561 acaggcgctc gccgaccaac gcatcgtctt ccgcgaagac ctcgtcatcg tcggtaatct
     4621 cagccaggag gccggcgccg aagccgcggc tgtcatccgg gaaatgcgac cgctgcccga
     4681 tgctgtcttt gtagctaacg acaactgtgc agtcggctgc atgacggccc tcaaacaaac
     4741 aggcatccgg atccccgagg acatcgcctt cgtaggattc aacaacgacc ccgtctccac
     4801 cgtcgtcgaa cccaatctca cgaccgtcaa ttaccccggt tacgaaatgg gacagatcgc
     4861 cgcacgccat ctcatcgacc acctcgccgg ctcatccaat atccaatcca ccaatacgat
     4921 catcctccgc agcgacctca tcatccgcga atcctcgctc acccggaaac caaacgcctc
     4981 atgaagcgtt tcgcctgcat cctgttcagc ctgcttcttc ttcggtcatc ccgcgtcctt
     5041 gcagaggacg gttaccgcct ctggctccgc tacgatagga tcgacgaccg tcaattgctg
     5101 cagcaatacc tccggaccat tccctcctgc accttccccg ccacgggcag cgagatcctc
     5161 ccggccgccc gtaacgagct gacgaatgct atcgccggtc tgctcggcca cccgcccgac
     5221 tatacaacga tcccttccgc caactccctc ctcatcggca cccccgcgtc tctccccttc
     5281 ctgacccgtc ttcactggga cgccgacatc caccgcctgg gcgacgaggg tttcatcatc
     5341 cgcacctaca ccctggacaa ccgctccggc atagctatcg tcgccgcctc cgataaaggt
     5401 gttctctacg gcgtcttcca attcattcgg cttctgcaga cgcaccaacc actgacgcac
     5461 ctcgatatca taagtatccc cacccgtgca tatcgcatcc tcgaccattg ggacaatatg
     5521 agccgcaacg tggaaagagg ttacgcgggc ctttcccttt gggactggtt cacgctccct
     5581 acctataagg accaacgata taccgactat gcccgtgcca atgcttctat cggcatcaat
     5641 ggcgcggtgc tcaataacgt gaacgcgaat tatctctttt taacccccgc attccttgaa
     5701 aaggcagccg cgctcgccga tatcttccgc ccctatggca tccgtgtcta cctctccgtc
     5761 aatttcgctt ctcccataca actcggcggg cttcacacag ccgatcccct cgacccggta
     5821 gtacgcgaat ggtggaaaaa gaaagccgat gagatttacc gctacatccc cgattttggc
     5881 ggcttcctcg taaaagccaa ttcggaaggc caaccaggcc cccagaacta cggacgctcg
     5941 catgcagacg gcgccaatat gatgggggaa gcgctggctc cgcaccatgg catcgtcatc
     6001 tggcgggctt tcgtgtataa tgcgaccgcg gccgatcgcg ccagacaagc ctacgatgag
     6061 ttccactcac tggatggaaa gttcgattcc aatgtcctgg tgcagatcaa aaacggtccc
     6121 ctcgatttcc agccccgcga acccttccat ccgctgttcg gcgctatgcc tgataccagg
     6181 ctgatgctgg aagtacagat cactcaggaa tacctgggag cctccaacca cctcgtttac
     6241 ctggcaccat tattcgcgga atgtatggac gccgacacct atacacatgg taacggtccg
     6301 accgtcggcc agtcgatcag cggcatggcc ggggttgcca atacgggtaa tgatatcaac
     6361 tggtgcggcc acatcttcgc gcaagccaac tggtacgcgt tcggccgcct cgcctggggt
     6421 gcaactcctg ccggcatcgc cgacgaatgg atccgccaga cctttaccaa caacaccacc
     6481 gccgtcgaca gcatacgaag aatgatgctg gcctctcgag agatcacagt agactacatg
     6541 aaccccgtgg gtttgaacat gatctttgca gaaggccatc attatgggcc ggggccatgg
     6601 tacgacaagg ctaaacgccc ggactggaac tcgacctatt accaccacgc cgattcggca
     6661 ggcatcgggt tcgaccgcac tcccaccggc agcaatatgc tcgaacaata tgcaccggga
     6721 gcccgcgccg aatgggaaga cgtgaagacc tgtcccgaca aatggctgct ttggttccat
     6781 catgtcccgt ggacctggaa aatgcactcc ggaaggaccc tatgggacga aatgtgttat
     6841 cgatatcaaa aaggggtcga ctccgtccgc tggatgcaac gttgctggca acgccttcga
     6901 ccggacatcg accaggaaag atggcgacag gtcgatatga aactccggat acaggaacat
     6961 gacgcagttt ggtggcgaaa tgcctgtttg ttgtattttc aaacattttc caggatgccg
     7021 atcccggccg gtgtcgaacc cccggatcat accctggagt attatgaaga cattcagtta
     7081 catgacgtgc ccggcacggg acgttaaaaa ctcgatcgga taatggacac atcacaaaaa
     7141 atagcgatca tcacaggcgg cggatcgggc atcgggctgg ccatcaccaa aaaattcgta
     7201 gagaacggca tcctcaccat catcgtcggg cgccaccggg acaagctcga cgccgcaaaa
     7261 aaacaattgg gcgacctctg ccatccaatg ccgcaggacc tcaatgacct caacgctatt
     7321 cccggtctgg tacgccgcat catcgaccac tacgaccaca tcgatatcct cgtcaacaac
     7381 gccggcatca atatgaaaaa agagctgcca gacgtcaccg atgaagattt cgaacggatc
     7441 ctgcttacca acgtacgatc cgttttcacg cttacacgcg aggtcgtgaa aaccatgctt
     7501 ccgcacggaa aaggaaatat tatcaatatc agctccatgg cctcccagta tggcattccc
     7561 aaggtcatcg cctatacggc ctccaaatcc gccatcgaag gaatgacgag agccatggcg
     7621 gtggaactgt cacccaaggg tatcctggtg aactgcatcg ctcccggttt catcgccacc
     7681 gatatgtccg caaaggcgct ggatacggat gccgaacgga aacaaaaggt cttctcgagg
     7741 acccccatgg gccggatggg cgatcccgcc gatatcggcg acgcagccct attcctcgcc
     7801 tccaaccagg cgaaatatat cacgggcgtc gtcctccccg tcgacggagg caattctatc
     7861 ggcttttaat catttgcact atgaagagaa tttctatcgt actcctgctg acgctgtcca
     7921 tcgggacagc gggagcccag agctttccca ttgtcacgaa gaacgaaggc gcgacacagc
     7981 tattcgtcga ccccaacgac cattggctgg tgaacagaac ggcgcaacta ctccaggaag
     8041 atatcgaaag ggtcaccggc atcaaacccg tcctcctcca cgcgcttccc gaccatcccg
     8101 tcgacaacct catcgtcatc ggcagcatgg acagctcggc catcgtaaaa caattgctcc
     8161 ggcgacatcg cctccccaaa ctcgccggca aatgggaagc atatagcatc aggacactgg
     8221 ccaatcccac gccgaccata gcccatgccc tcgtgatcgc tggcagcgac cgccgcggca
     8281 ccgcctacgc cgcactggag atatcccgcg aaataggcgt ctctccctgg tattattggg
     8341 cggacgttcc caccgcccac aaggacgaac tttatttcac ccgcgacagc atcctgttca
     8401 acggacccgc cgtcaaatac cgcggcttct ttatcaacga cgaggctccc gccctctcgg
     8461 gctgggtaaa agccaccttc ggtaatttca accacgtctt ttacgaaaag gttttcgagc
     8521 tcctcctccg cctgcgcggc aactacctat ggcccgccat gtggggtaac gcattcaacg
     8581 acgacgatac cctcaatccc gtactggcct ctgaatacgg tatcgtgatg ggcacctcgc
     8641 atcatgaacc catgctccgc gcacagcagg aatggaaacg ctacggccac ggaccctggg
     8701 actataccgt aaatgcgccc accctcgaca gcttctggga aaaaggcatc cggcacatgg
     8761 accaccacga aagcatcgtc acggtcggaa tgcgcgggga cggcgacaaa ccgatgacgg
     8821 aaggaagtaa catcgcgctg ctggaaaaga tcgtcgccga ccaacgcgca atcattcaaa
     8881 aagtgacgag ccaacccgca tcgcaaactc cccagtcctg ggcactttac aaagaagtac
     8941 aggattacta tgataaggga atgcgcgtac ccgacgacgt taccctcctt ctctgcgacg
     9001 acaactgggg agatgtccgg aaattgccga cgctcggctc caaaccgcga cagggaggat
     9061 atgggatgta ttaccatttc gactatgtcg gcggcccccg gaattataaa tggatcaaca
     9121 ccaaccagct gtcccgtgtc tgggaagaga tgcacctgac ccgcgaatac ggagatgaca
     9181 aaatatggat cgtcaatgtc ggcgatataa agccgatgga gttcccgaca cagttctttc
     9241 tcgactatgc ctgggacccg gccgcgcttc ccgccgatcg cctgcccgaa tacacctggc
     9301 actgggcggc cgaacaattc ggtgcgtccc acgccgcagc catcgccgat atcctgtcca
     9361 aatacacgaa atacaacgcc cgccgcaaac ccgagctcct ctcaccggat acctatagcc
     9421 tgccgaacta ccacgaggcc gaaacagtcg ccgatgacta taaaaccctc gcccaacgcg
     9481 ccgaatacat cgggcgcatg ttgcccgcgc aataccacga cgcatattac gagctcgtgc
     9541 tctacccggt cctggccagc gccaatctca acgagctcta ctacacggtc gcccgcaaca
     9601 ggctctacgc cactcaaggt cgcgccctta ccaaccggct cgccgccgtg gccgacagcc
     9661 tcttcggccg cgattcactc ctgtccctca ccttcaacca cgatatcgcc ggcggtaaat
     9721 ggcctcacat gatggaccag acccacatcg gctatacata ttggcaggaa ccgcgacaca
     9781 acaacaagcc gaagaccgac accatcgatc tcgccgccgc caccggcccc tcctggggcg
     9841 tattcccaac aaccctatcc ggtttcaacc catacgactt ccaaagccag tacatcgaag
     9901 tcttcagcag acgctccgct ccaactccat gcaccgtcac tcccgatgcc ccgtggatcg
     9961 atgcgcatac caccggtaac cccacctccc tccagcaacg ctggcaggtg tccatcgact
    10021 gggccaaagt ccccaccggc caacaccagg gcagtctcac gatcaccggc ccggacggca
    10081 ctcccctaac ggtaaccatc aacctgaata acccagcccc cgccccgcct ctggatttca
    10141 aaggattcat cgaaacgaac ggctgcgtcg ccatcgaagc gaacaatttc acgaaagctg
    10201 tcaccacccc ggccatcaac tggaccgtca ttcccgattt aggccgcacc ggctcggcgg
    10261 tagaagcatc tcccgtcact gctcccacac aaactcccgg tcctcatagc ccatgtctcg
    10321 aatacccggt ctggctcttc gataccggca ccgtcgccgt acaggcatat tgctcgccca
    10381 tcatcgcatt caacggcaaa ccgatccgct acgccgtcgg catcgatgag gaaaccccgc
    10441 aggtcatcga cctcaccacc ggcaacgaag cacgcggcac atgggataaa atggtttcgg
    10501 ataatatcaa ggtctctatc tcgcaccatg cagtctcgcg cccgggggca catatcctca
    10561 aattctatct cgtcgacccc ggcgtcgtac tccaacgcct cgttatcgat gcgggtggcg
    10621 tcaaaccatc ctatctgggt ccaccccaat caccgcgtcg cggcctcaag gatatatacc
    10681 atgacttctt ccctatcgga gtcgccgtca gcccgcgttc gctcgccggc cccgactcca
    10741 cgctgatcct ccaacagttc aacagcctca cccccgaaaa cgccatgaag atgggtccca
    10801 tccaccccga agaaaatcgc tataactgga aggatgccga cgccatcgtc gatttcgctc
    10861 agcgccacgg cctcctcgtc cgcggtcaca acctttgctg gcatcaacaa actccgccct
    10921 ggatgttcaa agacaccagc ggcggcgccg tgacgaaaga agtactgctg caacgattga
    10981 aagatcatat taacaccgta gtcagccgtt atagaggaaa agtgtatgcc tgggacgtcg
    11041 tcaacgaggc catcgacgac gacagcacca aattcctccg taattccgaa tggtaccgca
    11101 tctgcggcga agacttcatc gtcaaagcct tcgaatacgc gcacgcagcc gatccgtccg
    11161 cacagctttt ctacaatgac tataataccg aacggcccga aaaacgagaa cgtgtctacc
    11221 gcctgctcaa acagctcgtg gacgcgggag tgcctattac cggtgtcggc ctccaggcgc
    11281 attggtccgt caatgaaccc tccgcacacg acttgcagac agccatcgac cgattctcct
    11341 cgctcggcct caaggtccag gttaccgagc tcgatatctc catttacccc tgggaaaaat
    11401 tccgccgcac ccgccgtccg ggtgaacagg acgtctatac gcccgaaatg gaaaaacgcc
    11461 aggccgcgca atacaagatg gccttcgata tcttccgcgc tcaccgcgct tccatcaacg
    11521 gtattacatt ctggaatatc tcggaccgca atacctggct cgacaactac cccgtaacgg
    11581 gtcgcaagaa ctacccgctg ctattcgata ttaataacca gcctaagaaa gcatggtggg
    11641 aagtagtcga tttttaaatc ttttcgttat atttaatcat cgattgctgc cttgcgtccc
    11701 cgctgatgcg accaattcaa aacaactgct tattccggca acgccttcct tgccgaaatc
    11761 aatatttttt tatgcaccac cggttcctgt ccctcttcgc cgcactgctg ctgtttgcag
    11821 cggccaccgg ccaaaccctc cccttccgca attcatccct ccccaccgcc gacagggtca
    11881 ctgacctgct ctcgcgtctg acactacagg aaaaagtgag ccttctcggc tatcggagca
    11941 aatccgtcga ccgcctctcc attcccgcct acaactggtg gaacgaaggt cttcacggcg
    12001 tggcgcgcgc aggcgaagcc accgtttttc cccaggccat cggcatggcc gcctccttcg
    12061 acgctcccct gcttcggaaa cttgccgatg ccgtctccac ggaagcccgc gccaaataca
    12121 atctgtctac cggcctcggc cgccacgagc aatacatggg ccttaccttc tggtccccca
    12181 atatcaatat cttccgcgac ccacgctggg gccgcggaca ggagacttac ggggaagacc
    12241 ccttcctgac ggcgaccatg ggaagcgcct atgtcaaagg cctacagggc aacgactccc
    12301 gctacctcaa gacatccgcc tgcgccaaac attttgccgt tcacagcggt ccagagtcta
    12361 tccgtcacac tttcaatgcc atcatcgacg agaaagatct tcgcgaaacc tatctctacg
    12421 ctttcaaaag gctcgtggac tccggcgtag agtcggtcat gtgcgcatac aaccgtgtca
    12481 acggcgaacc ctgctgctcc ggtcccactc tcctgcatcg catcctccgc gaggaatggc
    12541 atttttccgg acatgtcgtc acggactgcg gcgcactcga tgatatcttc gaacgtcata
    12601 aagcccttcc gggacccgtc gaaacagccg ccgccgccat caaggccggt gtcgacctcg
    12661 attgctccga tatcctgcag gacgacgtac tcaaagccgt cgacaaaggt ctgcttaccc
    12721 gccgcgatgt tgactcggcg ctcgccccgc tgctccgcac ccagctgaag ctcggcttct
    12781 tcgatgacaa gaccgacatc cccttcagcg gctacggctc cgacagcgtc cataacgact
    12841 atcatatcgc ccttgcccgg cagatggctg ccgaaagcat agtcttgttg aaaaatgaaa
    12901 acaatgtcct tccgctccgg aaagattcgc tcgcttcgct gatggtcgtc ggtcccaacg
    12961 ccttctcgtt cgatgcgctg gcaggcaact atcacggcgt ctcctcccgg gtcgttactt
    13021 ttctggaagg cataactgcc gccaccggac ccgccgtaca tgtcgaatat gatcagggct
    13081 gcgattacaa ggactccatc catttcggcg gtacctgggc cgcgtccaac tgccagacga
    13141 ccgtagctat catcggcctc accccggtga tggaaggaga agaaggagac gctttcctgg
    13201 ccccgcacgg cggagaccgc accgacctgg cgctccctgc cgcccacatc gcctatataa
    13261 aggctctccg caaggccatc ggtcataaac ccctcatcgt cgtcgttacc gcgggcagcg
    13321 ccgtcgatct gtcccccctc gaaccctatg ccgacgccat cctcctggcc tggtatcccg
    13381 gcgagcaggg cggcaacgca ctggccgata tcctcttcgg aaagatctcc ccctccggac
    13441 gactgcccgt cacgttctac cgatccactg cagatctccc ctcttacaag gactatagta
    13501 tgaaaggccg cacctaccgc tactacgacg gaccggtaca ataccccttc ggcttcgggc
    13561 tcagctatac ttctttcgcc taccactggc agacgacgcc gaaaaaaacc tatacacccg
    13621 cggatacgct ctcattttcc gtcacggtgg acaataccgc caaccgcgac ggcgatgagg
    13681 tggtgcaggc ctatatcgtc taccccgatc tggaaaggat gccactaaaa gaattgaaag
    13741 cgatcggccg cgtgtcgatc cccgcccacg atgcccggcg gctcaccttc tcgatcccgc
    13801 tgcaacaact ccataaatgg gatctggccg ctcatgcctg gaaagtctat cccggaaact
    13861 ataagctcgt cctcggctcc gactcccgca acgaaaagct cgtcgcggaa tttacactac
    13921 accgatagcc ttatcttctt tttttccagt actcccgtca gcaaaacaat gagaaaagca
    13981 aagaccagcg atttgataac ccctgtcgcc cccgtgcgca gcaccacggg caattgccac
    14041 gctgatccca gcagcctgat gatcgcaaaa tgtatgtccg gtaacaaata acacgtcagt
    14101 gtgatcgtcc ccgccggcct tatccaccgg taccagttct gcttcccccg gacatctacg
    14161 ataaatgcca accccgccgc acaggcgaca cttataccgg tacagatcaa tatccacgac
    14221 ggcgatactt ccagcttcga tatgcctttc agcggcctca gccagaaacc ggtcgccacc
    14281 agcaaaggta cccacgccag caggcgataa atactcttca tcgcgagccc tttttccgac
    14341 atccggcgat agtataccgt ggtcatgacc ccggccatcg taaacgcgga caatcctccg
    14401 ctatccccca acgtgagcca ggacaccgcc ggtcgtatcc acccccagcc ggaacatacc
    14461 gctatcgaaa atccgaggaa atacaggaat accagaccca gcacccccaa ccgctccccg
    14521 atcaaaaggt acagtaaggc acaggtaaga taagcccacc cgatcagccc cagtataccc
    14581 caccaataat cattcatgcc cttcggcgcc tccggcgtcc ctcctttata gacgaaggcc
    14641 atcagcgcca gcgccgcgac accgatggtc ctgaaaacac ccttccgcca ccagggccat
    14701 tccttcggat ataccagcca gataaaaaag aacgcaagcg tgaccacgag caaccatccg
    14761 aatttcgata tccatgccgg accggtcgtg aactcttcat aattgacgag gaaataaccc
    14821 atgatcacca gggccaggct gcgacgcagg atatgccgca acgtatccca acgggtgaac
    14881 cccttttcat gcccatgccg gatggccaac ggtatcgaac acccgacgat gaacaggaat
    14941 gcggggaata tagtgtcggc gaatccaaga cggtcttcgc cctccctggc atgctccagc
    15001 cagtccggaa cgcggaaatc gaacaccagg tcgttgacga agatcatgaa gaacatcgtt
    15061 atcgcccgaa aggcgtcgat agaggccagg cggcgcgtag cacctttcat atgattcttg
    15121 tttacaggaa tttgcgcaaa taagaaagcc agtcgatcat tcggctcgta tacccatact
    15181 cgttatcgaa ccacgcgatc acctttacca gccggccgac agcggaagtt agtgtgccgt
    15241 ctacgacgca tgagtttgta tttcccttga tgtcaagcga aaccagcggc tcttcagtat
    15301 actccaatat
//
LOCUS       DASYSV010000399        19505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1611796, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000399 DASYSV010000000
VERSION     DASYSV010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 19505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19505
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611796"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VGQ51_12245"
     CDS             <1..549
                     /locus_tag="VGQ51_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9037392.1"
                     /translation="VELYGVVMTSDSLKAIPLVSIVTSDGRGTYTNDQGVFSLVVVKG
                     ERVEFTSIGYKPFTLIVPDTFHGNQYSIVQLMTTDAQYLPSTVIRARPTREQFERDFV
                     NTPVPDDDIEIARQNNDAAKRRILARGLPQSSGEAAATYLRQSAQKYYYSGQAPPMNI
                     FNPVAWADFIQAWKRGDFRNNN"
     gene            554..1459
                     /locus_tag="VGQ51_12250"
     CDS             554..1459
                     /locus_tag="VGQ51_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV9037393.1"
                     /translation="MQNISVIGAGTMGNGIAHVFAQKGFTVSLIDSQPQQLEKAIKTI
                     SANLDRQVVKGLLTEEGKKTTLQGIATYSDIASGTAKADLVVEAATENTQLKLTIFRQ
                     LDELAPAGCILATNTSSISITKIASVTRRPSSVIGLHFMNPVPVMKLIEVINGYATDA
                     QVTAQMVTLAGRLGKTPCVVNDYPGFIANRILMPMINEAIYSLYEGVAGVESIDTIMK
                     LGMAHPMGPLQLADFIGLDVCLSILRVLHEGLGLPKYVPCPLLVNMVTAGHLGIKTGE
                     GFYRYSPGSKDLVVSKRFTASSAIR"
     gene            complement(1472..3253)
                     /locus_tag="VGQ51_12255"
     CDS             complement(1472..3253)
                     /locus_tag="VGQ51_12255"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9037394.1"
                     /translation="MKKILLIPALALCAISILFFSACYKTDINAGSTSADPNHIPPDQ
                     TVIASLRGRVIDENGVPMEGASVVSGGITTTSDVNGLFSFSNISMSSRWGYVKVSKTG
                     YYTGSRSVITNGAASNYVNIQMIPRAETGSFAAGAGGVIVVQPGDSAAFGANMVVTAS
                     TNAAYTGTVHVFTKYLDPTDANLYKYMPGDLRGIGTDGKETALQSFGMLLVELQGDAG
                     EKLQIMNGQSATLTWAIPSGLQSVAPATIPLWYFNDSTGRWIQQGTAVRQGNSYVGKV
                     SHFSFWNCDAPVGTVNFKVRFKDQHGNPLPYTFFSFQSQTLGTRGGYTDSSGYAQGLI
                     LKGQTLVLSVSTECGTILGGANVGPALTDQDLGTIVVTITNNDLTLTGKVVNCAGNPV
                     DSGYINAAIDGLTYRAEVRAGAFTLPVLRCYSTTVPATLTAYDLVAMQRSGSSSVSVT
                     SGNVDAGTLSACQVAYNQYLNFSYPNISSSYTVPPDSVTWWMDPYSDVNLISANYANG
                     IKQTSTSITVTSFVGVGSYVIEDFLYQNFVTGQTFTGVGAYLSVTRYDPVGGYIVGSF
                     SGNLVSADSIGQLGHPLSGSFQVLRTK"
     gene            complement(3397..4020)
                     /locus_tag="VGQ51_12260"
     CDS             complement(3397..4020)
                     /locus_tag="VGQ51_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV9037395.1"
                     /translation="MNMIHGDKDIYLTIDKSVTAEFKDRGSRFIGHTFPATSVEECKN
                     QLNDIKKLHPKATHHCFAWRLGLDGLLFRTSDDGEPSGTAGRPILGQIDSKQLVNVQI
                     IVVRYFGGTLLGVPGLINAYRSAAALALQLTPLVQKPVEKEFVVQFDYTSIGEVMALA
                     KQYNCRILKQEMQLFCHMTLAIPKNRVLEISYKLQEIKNVELAPLQA"
     gene            complement(3998..5062)
                     /gene="ribD"
                     /locus_tag="VGQ51_12265"
     CDS             complement(3998..5062)
                     /gene="ribD"
                     /locus_tag="VGQ51_12265"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV9037396.1"
                     /translation="MHYFYALHSPETYMRRCLELASLGAGHVAPNPMVGAVLVFEDRI
                     IGEGYHRQYGGPHAEPECIDSVPEADRGLIGRSTLYVSLEPCAHHGKTPPCADLIIRT
                     GIPKVVIGCRDPFPEVNGKGIDRLRAAGVEVETGVLERECIGLNKRFFTFHLHHRPYI
                     VLKWAQTASGLIAGDGARWHISNEITNRLVHKWRSEEASILVGPRTALLDDPELTVRL
                     WTGPDPVRLVIDKELQLPFSLKLFDGKVRTIVFNNLQHGQDGHLEYYQLATDTSLIHQ
                     LVTALHRLKLQSVLVEGGARLLQSFIEEDYWDEARVITGEELEPPAGLPAPRLLHMRR
                     IGKETILSDTIRYYEHDSRG"
     gene            5082..5933
                     /gene="prmC"
                     /locus_tag="VGQ51_12270"
     CDS             5082..5933
                     /gene="prmC"
                     /locus_tag="VGQ51_12270"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV9037397.1"
                     /translation="MDWSQAQKELSDSLVPLHGQREATLIADWVMEALSGRRRLERLL
                     MKTEPLSPETLATYRGYRDELLAHRPVQYVLRECWFAGMKFFVDEGVLIPRPETEELV
                     EWIARELSDAVSGSLLDVGTGSGCVAVTLAKKLPGMNVHAVDLSDAALAVARRNAHDL
                     DAPVVFHQLDFLDRTGWDLLPPLRWLVSNPPYVPLREKETMDPHVVKSEPDIALFVPD
                     EDALVFYRALGEFAGRRLERGGSLYVEIHEERGPAVRELLLQLGAIEVIIRKDFQGKD
                     RMIKAVW"
     gene            complement(5943..6347)
                     /locus_tag="VGQ51_12275"
     CDS             complement(5943..6347)
                     /locus_tag="VGQ51_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEV9037398.1"
                     /translation="MNLRRRIKDDAEVHTGPLNDILFILLMFFLIVSTLANPNVVKVS
                     QPKSKGDTKAKQTVVVSIDANKQFYVGTMHVTLDELKAKLQPFLAKETEQPSVVINAD
                     KTVPIDDVVAVMRVARDLGAKTVLAVSRSGAK"
     gene            complement(6402..7109)
                     /locus_tag="VGQ51_12280"
     CDS             complement(6402..7109)
                     /locus_tag="VGQ51_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEV9037399.1"
                     /translation="MNLFFLQVLIDSTKIKDSLATVAQAAAQQKQNLWDILVKGGPLM
                     VPLAIVLVIAIFFFFERYIAIKKASRMDDNFMSIIRDHIVNGNIQAARSLSKNTDTAV
                     AKMIDKGIQRIGKPIEAIERSMENVGKLELYKMEKNTSILSMVGYIGPLFGFLGTIAG
                     MLQLFYDIANSPGDYGISNIAAGIYVKMVSSATGLIIGIIGYLMYRYCMAQIDKATNR
                     MEVASADFIDILQEPTR"
     gene            complement(7241..8197)
                     /locus_tag="VGQ51_12285"
     CDS             complement(7241..8197)
                     /locus_tag="VGQ51_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV9037400.1"
                     /translation="MESSNLVLIIVVIIALAILFSCWVTVNQGYIDVITMFGKYQRVM
                     RPGLNFKIPFLEQVYKKISIQNRSVELEFQAVTADQANVYFKSMLLYAVQNAEEDTIQ
                     KVAFKFISDRDLMQALTRTIEGSIRSFVATKRQAEILGLRKEIVEFVKDQIDHSLEDW
                     GYHLMDLQINDITFDKAIMDSMSRVVASNNLKAAAENEGQALLITKTKAAEAEGNAIK
                     ISAEAEKEAARLRGQGVALFRQEVAHGMSEAAEEMKRANLDTNVILFSMWTEAIKNFA
                     EVGKGNVIFLDGSGEGMQKTMNQIQAIIMQQATPPAPLPVNR"
     gene            8343..9326
                     /locus_tag="VGQ51_12290"
     CDS             8343..9326
                     /locus_tag="VGQ51_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HEV9037401.1"
                     /translation="MRTTYTLIIIGGGPIGIACALEAQKEGIDYLILERGCLVNSLYR
                     YPVNMTFFSTSERIEIGGVPFVSNNVKPNRGEALEYYRRVAVSHQLHIHLQEGVRELH
                     SRGGSFEVVTDLRTYTATYVVLATGFYDIPVLLDIPGESLPKVTHYYKEPHFYAMQRV
                     IVVGASNSAVDAALETWRKGAEVTMVIRGEGIGPRVKYWVKPDIENRIREGSIKAYTN
                     SSLAAIRDGEVDIRTPEGIVTIPNDYVIALTGYRPDFDFLRRAGIQLSDDSRQQPFYD
                     PATMETNIPNLFLAGVICGGMNTHSWFIENSRIHAGLIMRPIAQRSHAAAG"
     gene            complement(9306..10565)
                     /gene="pepT"
                     /locus_tag="VGQ51_12295"
     CDS             complement(9306..10565)
                     /gene="pepT"
                     /locus_tag="VGQ51_12295"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV9037402.1"
                     /translation="MFENYAFTVAERFIRYARIDTQSDPGSKTCPSTEKQKDLGRLLV
                     EELRSAGLDDAQLDEFGYVYATLPSNTDKQVPVVCFCAHMDTAPDCSGQGVNPIVHHG
                     YDGGDIVLPDDPTQVIRVSEHPYLMSRKGDDLITAGGTTLLGADDKAGIAVIMDMVHF
                     LLSHPGQKHGKIRILFTPDEEIGRGVDKVDLVRLGADVGYTLDAGERATLEDETFSAD
                     AMTIVFKGISAHPGYAKGKLVNAIKVASHFVDSLPKGEWSPETTDDRYGFVHPVRISG
                     MVERATVEFIIRDFITPRLAAYEAYLRDKVADTLALVPGATAEITVSEQYRNMKEVLD
                     RHPVITQYAMEAIRRAGMEVRKSSARGGTDGSRLSFIGLPCPNLFTGEMAFHGKHEYV
                     SVQDMQKSVEMIVHLACIWEELSGGRM"
     gene            10596..11522
                     /locus_tag="VGQ51_12300"
     CDS             10596..11522
                     /locus_tag="VGQ51_12300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV9037403.1"
                     /translation="MTEAIVKKPVIRQGWLRVLIFGALFIILTLLIAIPAAMLVAGIP
                     GDQLKQQPIATLSGLLTGNYLWLMILVELAISVISVGVCRLWLDRRPLVDSGWFADGF
                     TGEAITGIFMGPALLGIVAIVLLLTGHVEWTDVVWAPSSLFVSLGLMALIAFSEELVF
                     RGYVLGNLLETLPNKWVALIIASALFAGFHFPNPSIYALAFVNLFLAGLLLGINYIYT
                     KNLWFSFFFHLSWNYFAGPIMGFHVSGLAQPSLLQADTKGDLLITGGDFGLEGSILTT
                     CVAVIAFFVLLWAFERKYASAPAPPLTPSTQL"
     gene            complement(11538..11993)
                     /locus_tag="VGQ51_12305"
     CDS             complement(11538..11993)
                     /locus_tag="VGQ51_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV9037404.1"
                     /translation="MAQEVKAGDTVKVHYHGRLTDGTTFDSSEGRQPLEFEVGGGMVI
                     RGFDEGVMGMTVGQKKTIEIPVEDAYGPRDPEAIIEFPIERFPAEMKPEVGMQLFMNN
                     GAGQQIPVVIVEVKPEVVVLDANHQLAGQDLIFDLELVDIKSPSRIIMP"
     gene            12096..13259
                     /locus_tag="VGQ51_12310"
     CDS             12096..13259
                     /locus_tag="VGQ51_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEV9037405.1"
                     /translation="MRKALIVILIILFIQPITRAQIVIGAEQMDRYLPLLKGRSVAIF
                     ANATSVVGNGNTHLVDTLLKKGIHIVKLFSPEHGFRGDADAGAKVGNQVDSKTGIPII
                     SLYGAKTKPTAEDLADVDIMLYDIQDVGVRFYTYIGSLQRYMEAAIENHRPLVILDRP
                     DPNGSYVDGPVLDTAYRSFVGMQPVPIVYGMTIGEYARMLIGEHWVHADPGFKLTIIP
                     CRNYTHRSKYILPVKPSPNLPTMQSVYLYGSLCLFEGTDVSLGRGTTKPFQQFGNPDF
                     PKDLYSFTPESMPGATNPPQLGKTCYGYDLSAVSIPATGLSFQWVLRAYSLSPNKDSF
                     FLRNGRGFDRLAGGPVLRQQIRDGLSEADIRKSWEPALQQFKAIRKKYLLYAD"
     gene            13249..14166
                     /gene="fmt"
                     /locus_tag="VGQ51_12315"
     CDS             13249..14166
                     /gene="fmt"
                     /locus_tag="VGQ51_12315"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV9037406.1"
                     /translation="MPTDPRIVFMGTPEFAVASLDAILAAGYRVVAVVTAPDKPAGRG
                     MQLSVSAVKKYALEKGLHLLQPEKLKNPAFLEDLRALQAELQIVVAFRMLPEAVWNMP
                     PMGTVNLHGSLLPQYRGAAPINWAVINGEPVTGVTTFRLKHAIDTGNILLQERFPISE
                     DQTAGEVHDKMKMIGAGVLVRTIDGLVDGSLHETPQENPPDLKPAPKIVTETCRIDWN
                     KPAREVHNLIRGLSPFPTAFTTLDGRMLKIYRSEIEIKPPAVAPGIHDTDGKTYFRWA
                     ASDGYIRATEVQLEGKKKMAIEDFLRGYR"
     gene            complement(14163..15164)
                     /locus_tag="VGQ51_12320"
     CDS             complement(14163..15164)
                     /locus_tag="VGQ51_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV9037407.1"
                     /translation="MRIPAAVLLLLTVFFSDSAHGQSGELLIQGQTGKLYLEHTVVAK
                     ENWYSVGRLYNVSPKTLAPYNHHTLQQPLTIGQTLEIPLTTTNFSQDGVKTASETLVP
                     VFHIVREKEWMYRISVNHNKVPIPTLEKWNHINGDQLHPGLHLIVGYLKVKTALSALA
                     TSVSAPVAAATPPPVSPVVHQALSTGNDAASTPVKTDSPAGTRVHPQATSPVPAQETT
                     TTVKPPAVIPNAPHFDGGVFRGDFSDEGHSVNGQAGVFKSTSGWQDGKYYALMNNVNV
                     GTIVKVTDPATGKSVFTKVLGQLPDMKESAGLTIRISNAAAAELGQGEGKFAVQVIY"
     gene            complement(15199..16563)
                     /locus_tag="VGQ51_12325"
     CDS             complement(15199..16563)
                     /locus_tag="VGQ51_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV9037408.1"
                     /translation="MSSLLSKQFLASLRGLPGYDASAFEAVHAGDRQVTSIRVNPDKT
                     GDSGPAGATRVPWSSWGYYLAVRPYFTFDPLLHAGAYYVQEASSMFLEQALRQTADVT
                     RPLRVLDLCAAPGGKSTLLQAALAAGSLLVSNEVIKSRVPVLEENMIKWGGVNSVVTN
                     NDPRDLSRLENYFDVIVVDAPCSGSGLFRRDPETMKEWSPAQVQLCSGRQQRILSDIW
                     PALRQDGVLIYSTCSYSREENEDILDWTAEEFDATPCRLLLSPEWNIVESEGIQKTWG
                     YRFYPDRLNGEGFFIGALRKNGGGTFSPPRRTAQPERAGKKELESLHKWVKPETALFF
                     FHHGDQIHVLPEPLAAELPILQSACYLKTAGVVLGTPSAKEFIPDHGLAMSTVIHPDL
                     PAIGLTHEQALNYLRKEEVRTETEHRGWSLVRYAGHNLGWVKVLPSRWNNYYPKQWRI
                     LKRT"
     gene            complement(16635..17462)
                     /locus_tag="VGQ51_12330"
     CDS             complement(16635..17462)
                     /locus_tag="VGQ51_12330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV9037409.1"
                     /translation="MYSPLRLAGKYFRYYLTAANGKGHGVHSPFVFDFVTHVLNDRRI
                     YAEYAPIEMLRSRLSGDTGVLPIEDLGAGSVSGAGTQRRVLDIVRRSAKPQRLGRLLF
                     RIARHYRPGVMVELGTSLGLSTAYLAAGAGLDRAEGEAGAPGSRLYTIEGSAALAEAA
                     AGNLRSLGLDARIVTGNFDDELAPLLQTIPPVDLAFIDGNHRYEPTMRYFESLFAHAS
                     PGAVLIFDDIYWSGEMERAWEDIRRDPRVMLTIDLFFLGFVFIREEFRVKQDFTIRF"
     gene            complement(17468..17971)
                     /locus_tag="VGQ51_12335"
     CDS             complement(17468..17971)
                     /locus_tag="VGQ51_12335"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV9037410.1"
                     /translation="MIISLIVAATTNNVIGKDNKLLWHLPNDMKFFKNTTWGMPVIMG
                     RKTFEALAGEPLPGRFNFVITRQNEWRPRREKVEVVHDLTAALHQAGQTDAKEAFIIG
                     GGQIYAEAMSVADKIYMTRIHTVLEGDTFFPVIDEKDWTMSGNLDFQPDDKHAFAYSF
                     QLWERKK"
     gene            18084..18401
                     /locus_tag="VGQ51_12340"
     CDS             18084..18401
                     /locus_tag="VGQ51_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037411.1"
                     /translation="MYSEKILNTAYSFAAAVVVFGAWAKLEQKEFGSIALTVGLLVES
                     GIFMIYGLMEWRSRPRAAGSPEQPETPLAKMSETPTPENAQELARTMERTNRILTKIF
                     RAE"
     gene            complement(18406..19215)
                     /locus_tag="VGQ51_12345"
     CDS             complement(18406..19215)
                     /locus_tag="VGQ51_12345"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV9037412.1"
                     /translation="MQQYLDLLRHILDKGVRKTDRTGTGTLSCFGHQLRFDLTSGFPL
                     VTTKKLHLKSIVYELLWFLRGETNTRFLKEHGVSIWDEWADADGELGPVYGKQWRSWE
                     GAGGRTVDQISQLIDQIRKTPDSRRLIVSAWNVTDLPEMALMPCHVLFQFYVAPDGEG
                     RQRLSCQLYQRSADVFLGVPFNIASYALLTLMIAQVCGLEPGEFVHTFGDVHLYSNHI
                     EQARLQLTRTPYPLPVMKLNPAVKDIFAFRFEDFTLENYISHPAIKAPVAV"
     gene            19258..>19505
                     /locus_tag="VGQ51_12350"
     CDS             19258..>19505
                     /locus_tag="VGQ51_12350"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEV9037413.1"
                     /translation="MRLLRVNNFSSEPSLRPGRFFLTTRIKKPGRFDLISYLGILSQS
                     GKTILTHRLCGIPGDTLELKAGELYVNGQEADGSLALK"
BASE COUNT         4391 a         5558 c         5048 g         4508 t
ORIGIN      
        1 gtagagcttt acggtgtagt catgacgtcc gatagcctga aggcgatccc gctggtcagc
       61 atagtgacct cggatgggcg gggcacatat acgaacgatc agggcgtgtt ttcgctcgtg
      121 gtggtgaaag gagaaagggt cgaatttacc agcatcggct ataaaccatt tacgctcata
      181 gtcccggata cttttcatgg caaccagtat agtatcgttc aactgatgac cacggatgcg
      241 caatatctac cttcaacggt tatccgcgcc cgaccgaccc gtgagcagtt cgagcgggac
      301 tttgtcaata cgccggtccc ggacgacgat atagaaattg cacgacagaa caatgacgcc
      361 gccaaacgac gaatactggc gagaggtctt cctcaaagct cgggcgaagc tgccgccact
      421 tatctgcgac aaagtgcgca aaaatattat tactcaggac aggcgccacc gatgaatatc
      481 ttcaaccccg tggcatgggc cgatttcatc caggcgtgga aacgtggcga tttccggaat
      541 aacaactaac tgcatgcaaa atatctccgt catcggcgcc ggtaccatgg gcaatggtat
      601 cgcgcacgtc ttcgcacaaa aaggatttac cgtctctttg atcgattcgc agccccagca
      661 gctggagaaa gcaataaaga ccatctccgc caatttagac cgtcaggtcg tcaaaggctt
      721 gttgacggaa gaaggaaaaa agaccacgct gcaaggcatc gccacctact cagacatcgc
      781 gtccggcacc gccaaggccg atttggtcgt tgaagccgcc accgaaaata cgcagctgaa
      841 attaaccatc ttccgccagc tggacgaact ggcccccgcc ggatgtatcc tggcaaccaa
      901 tacctcatcc atctccatca caaagatcgc atcggtgaca cggcggccct cttccgtcat
      961 cggtcttcac ttcatgaacc cggtgcccgt gatgaaactg atcgaggtga tcaatggtta
     1021 tgccaccgac gcgcaggtaa ccgcgcaaat ggtgaccctg gccggccggt tgggcaagac
     1081 gccctgtgtc gtcaatgatt atcccggatt cattgcgaac cgtatcctga tgccgatgat
     1141 caatgaggcc atctatagtc tgtatgaagg tgtagccggg gtcgaatcga tcgatacgat
     1201 catgaagctg ggcatggcgc atcccatggg acctctgcag ctggcggatt tcatcgggct
     1261 cgatgtctgt ctctccatcc tacgcgtttt gcacgaaggt ctcggtctgc ccaaatatgt
     1321 tccctgtcca ttgctcgtga atatggtgac ggcgggacat cttgggatca agacgggaga
     1381 aggattctac cgctattcgc cgggtagcaa agacctggtg gtcagcaaac gctttactgc
     1441 gtcttccgcg atcagataat aagcccacgt tctacttcgt ccgtaagacc tggaacgagc
     1501 cgctcaacgg gtggccgagc tgcccgatcg aatccgccga gactaaattc ccgctaaacg
     1561 agcctacgat ataaccgccc accggatcat accgggtaac cgataaatag gcgccgaccc
     1621 cggtgaacgt ctgacctgtt acgaaatttt gataaagaaa atcctcgatc acatacgatc
     1681 cgacgcctac gaaactcgtc acggtgatcg acgtgctcgt ctgttttata ccgttggcat
     1741 aattggcgct tattaaattc acatcgctgt aaggatccat ccaccacgtc accgaatccg
     1801 ggggaaccgt atacgaactg cttatattcg gataggagaa attcaaatat tgattgtagg
     1861 ctacctggca cgcggaaagc gttccggcat ccacgttacc cgaggttacc gaaacagaag
     1921 agctgccgct ccgctgcatc gcgacaagat cataagccgt aagggtcgcc ggaacggtag
     1981 tagaataaca acgtaggacg ggcaatgtga aagcccccgc acgcacttcc gcccgatagg
     2041 tcagcccatc gatcgctgcg tttatatatc cgctgtcgac cggattaccg gcacagttga
     2101 ccactttccc ggtcagtgta agatcgttgt tggtgatggt tacgacaata gtaccgaggt
     2161 cctgatccgt cagggccgga ccgacattag cccctcccag tatcgttccg cattctgtag
     2221 aaacgctcag caccagcgtc tgtcccttga gtatcaaccc ctgagcataa ccgctggagt
     2281 cggtgtaccc gccgcgcgtg cccaacgttt gcgactggaa agaaaagaag gtatatggca
     2341 gcggattgcc atgctgatct ttaaaacgta ccttgaaatt caccgttccg accggggcat
     2401 cgcagttcca aaagctgaaa tgggaaactt tacccacgta gctgttcccc tgacggacag
     2461 ccgttccctg ctggatccag cggcctgtgc tatcattgaa ataccacaac ggtatagtcg
     2521 ccggcgcgac gctttgcaaa cccgacggga tcgcccatgt cagggtagcg ctctgcccgt
     2581 tcattatttg aagcttctca cccgcatcac cctgcaactc gaccagcaac atcccgaaac
     2641 tttgaagggc cgtttctttt ccgtcggtac cgataccacg cagatcaccg ggcatatact
     2701 tgtacagatt ggcgtcagtg ggatcgaggt atttcgtaaa aacatgcacg gttcccgtat
     2761 aagccgcgtt ggtgctcgcc gtaaccacca tattcgcgcc gaaagcagcg gaatcgccgg
     2821 gctgaacgac gatgacacca cccgcacccg cagcgaaact tcctgtttcc gcacgcggga
     2881 tcatctgtat attcacgtaa ttggacgccg caccattggt aatgacgctc ctcgatcccg
     2941 tatagtagcc tgttttggac accttcacat acccccatcg gctggacata ctgatattgg
     3001 aaaaagaaaa aagcccattg acgtcgctag tcgtggtgat acccccgctg acgacagaag
     3061 ccccctccat gggaacgcca ttctcatcga tgacccgtcc tctcagactg gcgatgacag
     3121 tttgatccgg gggaatatgg ttgggatctg cggaggtact gccggcgttg atgtcggtct
     3181 tataacaagc gctgaaaaac aggatcgaaa tggcgcaaag cgccagggcc gggatgagta
     3241 gtatcttttt cacgggggcg gatttatgtg ccgggaatac gcttcataga aaatagcgca
     3301 ttcccggcaa agtgctaatg tctttccttg agccgccctt ggcggctcct taggtattgc
     3361 taaaacgaaa tgtccaccct aaaattatcc tacctctcag gcttgcaaag gagccagctc
     3421 tacgttcttg atttcctgca acttataact tatttcgagc acccgattct taggtatggc
     3481 cagggtcata tgacaaaaaa gctgcatctc ctgtttgagg atccggcaat tgtattgttt
     3541 ggccaaagcc attacctctc cgatgctcgt atagtcgaat tgaaccacga attccttctc
     3601 gaccggcttc tggaccagtg gtgtcaactg aagcgccaat gcggccgccg accggtaggc
     3661 gttgatcaat cccggtacgc cgagcagggt acctccgaaa taacggacca cgatgatctg
     3721 tacgttgacg agttgtttgc tgtctatctg tccaaggata gggcgcccgg cagtcccgga
     3781 aggttcgccg tcatcgcttg tccggaacag aagcccatcc agccccaacc gccaggcaaa
     3841 acaatgatgg gtagcctttg gatgcaattt ctttatgtcg ttgagctgat tcttacactc
     3901 ttccacagag gtggccggaa aggtatggcc gatgaaccgg cttcccctgt ccttgaattc
     3961 cgcggtaacg ctcttgtcga tggtcaaata aatgtcctta tccccgtgaa tcatgttcat
     4021 agtaacgaat ggtgtccgat aaaatagttt ccttccctat gcgacgcata tgcagaagcc
     4081 ggggagcggg cagaccggcc ggcggctcga gctcttcacc ggtgatcacc ctggcctcat
     4141 cccaataatc ttcctcgatg aaggactgca gcaggcgcgc tccgccttcg acgagcacgc
     4201 tctgtagttt tagccgatgc agggccgtca ccagctgatg aatgagactt gtatcggtgg
     4261 ccagctgata atattccagg tgtccgtcct gtccatgttg aaggttattg aagacaatgg
     4321 tcctcacctt cccgtcaaat agcttcagcg agaacggcag ctgcagctcc ttgtcgatga
     4381 ccagcctcac cggatccgga cccgtccaca gccggacggt caactcagga tcgtccaaca
     4441 gggccgtacg gggtcccacc aggatcgacg cttcttccga ccgccattta tgcaccagcc
     4501 ggttggtgat ctcattggaa atatgccacc ttgcgccgtc accggcgatc aatccgctcg
     4561 ccgtctgtgc ccatttcagg acgatgtagg ggcgatgatg aagatggaag gtaaaaaatc
     4621 gtttgttcag cccgatgcac tctctctcca acacacccgt ctccacctcc actcctgccg
     4681 cacgaagccg gtcgatgccc ttaccgttca cttccgggaa aggatcccgg cagccgatga
     4741 ctacctttgg aatacctgtc cgtatgatca ggtcggcaca tggcggagtc ttcccatgat
     4801 gagcgcatgg ctccagcgaa acatatagcg tcgaccgccc gataagcccc ctgtcggcct
     4861 ccggtaccga gtcgatacat tccggttcgg cgtgaggccc tccatattgg cggtgatagc
     4921 cttcgccgat gatgcggtct tcaaaaacca gcacagcccc caccatcggg ttgggagcga
     4981 catggccggc tcccaggctg gcaagctcca aacaacggcg catatatgtt tccggcgaat
     5041 gcaaggcgta aaaataatgc aaatgagact atttttgtcg gatggattgg tcacaggcgc
     5101 aaaaagagct aagcgattca ctggtcccgc tgcatgggca acgggaggct accctcatcg
     5161 ccgactgggt aatggaggcg ctcagcggcc ggcggaggct ggaaagactg ctaatgaaga
     5221 cggaaccgtt gtcccctgag accctggcga cataccgggg ataccgggat gagcttctgg
     5281 cgcatcgccc cgtacagtat gtattgaggg aatgctggtt tgccgggatg aagttcttcg
     5341 tggatgaagg tgtattgatc ccgagaccgg agaccgaaga attggtggaa tggatcgcaa
     5401 gagagctctc cgatgctgtt tccggctccc tgctcgatgt cggtaccggt agcggttgcg
     5461 tcgcagttac gctggcgaag aaattaccgg ggatgaacgt ccatgccgtg gacctcagcg
     5521 atgctgcgct ggcggtggcc cgacgcaatg cgcacgatct ggatgcgccc gtcgtattcc
     5581 atcagctgga ttttctcgat cggaccggat gggatttgct tcctccgctg cgctggctgg
     5641 tcagcaatcc tccttatgta cccctgcgcg aaaaggaaac catggatccc catgtggtga
     5701 agtcggaacc tgatattgca ttgttcgtac ccgatgagga tgcgttggta ttctatcgcg
     5761 ctcttggtga atttgccggt cgccgattgg agcgtggggg gtcgctttat gtcgagatcc
     5821 atgaagaaag ggggcctgcc gtgcgggaat tgctactaca gctgggggca atcgaagtca
     5881 tcatacggaa agactttcag ggaaaggacc ggatgataaa agcggtctgg taactgccgc
     5941 ccctatttcg ctccacttct ggataccgcc aggacggtct tcgcgcccag gtcgcgcgct
     6001 acccgcatga cggccaccac gtcgtcgata ggcaccgttt tgtcggcatt gatcacaacg
     6061 gaaggttgtt ccgtctcctt ggcgagaaag ggctgtagct tggcctttag ctcatcgagg
     6121 gtaacatgca tcgtaccgac atagaattgt ttgttcgcat ctatcgatac gaccaccgtt
     6181 tgcttggcct tggtgtcgcc ctttgacttg ggctgcgaca cctttaccac attggggttg
     6241 gccagtgtcg aaacgatgag gaagaacatt agaaggatga acaggatatc gttcaatggt
     6301 cccgtgtgca cctccgcgtc atcttttatt cgtcttctta aattcatgtt tatcagttgt
     6361 cttccgaatg cgacggagcc caaccccgac ccgcattcgc actatcttgt aggctcctgc
     6421 aggatatcga taaaatcggc actggccacc tccattcggt tggtagcctt atctatctgc
     6481 gccatgcagt aacgatacat caggtagccg atgatcccga tgatcagccc cgtcgccgaa
     6541 gagaccattt ttacatagat gcccgcggcg atattcgaaa tgccgtagtc gccgggggag
     6601 ttggctatgt cgtaaaacaa ttgcaacatc cccgcgatcg ttcccaaaaa gccgaacaac
     6661 gggccgatat atcccaccat cgacaggatc gacgtgttct tttccatttt gtacagttcc
     6721 agcttgccga cgttttccat gctccgttcg atggcctcga tgggcttgcc tatccgctga
     6781 atgcccttat cgatcatttt cgcaacggcc gtatcggtat tcttggacag gctgcgtgca
     6841 gcctggatat tgccattgac gatatgatca cggatgatgc tcatgaagtt gtcgtccatt
     6901 ctggaagcct tcttgatggc aatataccgt tcgaagaaga aaaagatggc gatcaccagg
     6961 acaatggcca gcgggaccat cagcggcccc cctttgacca ggatatccca taaattttgt
     7021 ttttgctgag ccgccgcctg ggccaccgta gccaggctgt ccttgatctt cgtggaatcg
     7081 ataagaacct gtagaaagaa tagattcatg tcgtaaatgt aaagatttag gcctataacg
     7141 gcccccgggc cgttttgttt tttaaggaaa tgctaaaatc gcgcttccgg tctaccagca
     7201 aaaattagac cgtcgcgcaa caggtctgtc gcgccacggt ctacctattc accggcagcg
     7261 gcgccggcgg ggtcgcctgt tgcatgatga tcgcctgtat ctggttcatc gttttctgca
     7321 tcccttcacc gctaccatcg agaaaaatga cattcccctt acctacctcg gcgaaattct
     7381 tgatagcctc ggtccacatg ctgaaaagga tcacattggt atcgagattg gcacgcttca
     7441 tctcttccgc ggcctcgctc ataccgtggg ccacctcctg gcggaaaagg gcgacaccct
     7501 ggccacggag acgggccgcc tctttctctg cctcggcgga gatcttgatg gcatttcctt
     7561 cggcttcggc cgccttggtc ttggtgatca ggagcgcctg gccctcgttc tcggccgcgg
     7621 ctttcaggtt attgctggca accaccctgc tcattgaatc catgatggcc ttatcgaagg
     7681 tgatatcgtt gatctggaga tccatcaaat gataacccca gtcctccagc gaatgatcga
     7741 tctggtcttt tacgaactct acgatctcct ttcgcaaccc gagtatctcc gcctggcgct
     7801 tggtggccac gaatgaccgg atgctgccct cgatggtgcg tgtcagcgcc tgcatgagat
     7861 cgcggtcact gatgaatttg aacgccactt tttgaatggt gtcttcctcc gcattttgaa
     7921 cggcgtataa cagcatgctt ttgaaataca cgttcgcctg atcggcagtc accgcctgga
     7981 actccagctc cacgctccgg ttttggatcg atatcttctt atacacctgc tcgagaaaag
     8041 ggatcttgaa attcaaaccg ggacgcatga cgcgctggta tttaccgaac atggtgatga
     8101 catcgatata tccctggttg accgttaccc aacagctgaa gaggatggcc agggcgatga
     8161 tcaccacgat gatcagtacg agattggagg attccataac cagacagaat tttatgtgcg
     8221 gaaaatacgc ttctgccaaa actagcgcat tttccgcaaa gcgctaatgt cggaaagccg
     8281 ctccgcgact ttatgggtga atgtacataa atttgcgtct accggcagtt taatttaacg
     8341 aaatgcgtac gacttacacc cttatcatta tcggcggcgg acccatcggc atcgcctgcg
     8401 cactggaggc tcaaaaagag gggatcgatt atctcatcct ggagagagga tgcctggtca
     8461 attcgctcta tcgttatccg gtgaatatga cctttttctc gacgtccgag aggatagaga
     8521 tcggcggggt gcccttcgtc agcaataatg tcaagcccaa ccgcggagaa gctctggaat
     8581 attatcggcg cgtagcggtc agtcatcagt tgcatatcca tctccaggaa ggcgtgcgcg
     8641 aactccacag ccggggcggg tcattcgagg tggtaaccga cttgcgcacc tatacggcta
     8701 cgtatgtcgt cctcgctacc ggcttttacg atatccccgt cctgctggac atccccggag
     8761 agtcgctgcc gaaggtaacg cactactata aagagcctca cttctacgct atgcagcggg
     8821 tgatcgtggt aggggccagc aattccgcgg tggatgccgc actggaaacc tggcgtaagg
     8881 gcgcggaagt gaccatggtc atccgagggg aaggcatcgg tccccgggtg aaatactggg
     8941 taaaacccga tatcgaaaat cggatcaggg aaggcagcat caaagcctat acgaattcct
     9001 ccctggcggc catacgcgac ggagaggtgg acatccgcac tcccgaaggc atcgtcacca
     9061 ttcccaatga ctatgtgata gcgcttaccg gctacagacc cgatttcgat ttcctgcgcc
     9121 gcgcaggcat ccaactctcg gatgattccc gccagcaacc tttctatgac cctgcgacga
     9181 tggagacgaa tatcccgaac ctcttcctcg ccggtgtcat ctgcggcggc atgaatacac
     9241 atagctggtt catcgaaaat tcaaggatcc acgccgggtt gatcatgcgc cccattgccc
     9301 aacggtcaca tgcggccgcc ggatagctcc tcccagatac atgcgaggtg cacgatcatc
     9361 tccacagact tttgcatatc ctgtacgctc acatattcat gcttcccatg aaatgccatc
     9421 tcgcccgtga ataagttggg acacggtagt ccgataaaag ataaccgcga tccatccgtc
     9481 ccgccccggg cgctgctttt tcggacttcc attcccgcgc gccggatggc ctccatcgca
     9541 tattgcgtta tcacgggatg tcggtccagc acctccttca tattccggta ttgctcgctg
     9601 accgtaatct ccgccgttgc acccgggacc agcgccagcg tatcggccac cttgtccctg
     9661 agatacgctt cgtatgcggc aagccgggga gtaataaagt ccctgatgat gaattccacc
     9721 gtcgcacgct caaccatacc cgatatacga accgggtgga caaatccata acggtcgtcg
     9781 gtcgtctcgg gcgaccattc cccttttgga agcgaatcga cgaaatgcga cgcgaccttg
     9841 atggcattga ccagcttgcc cttcgcatat ccgggatgcg cggagatacc tttaaaaacg
     9901 atggtcatcg catctgcgga aaaggtctcg tcttcaagcg ttgcccgctc gccggcatcc
     9961 agcgtatatc cgacatcggc ccccagacga accaggtcga ccttatccac tcccctgccg
    10021 atctcctcat cgggcgtaaa aaggatcctg atcttgccat gtttctgacc gggatgcgac
    10081 agcaggaaat gaaccatgtc catgatgacc gcaatgccgg ccttgtcatc ggctcccagc
    10141 agggtcgtgc ctcccgcggt aatgagatca tctcctttcc gcgacatcag gtatgggtgc
    10201 tcgcttacgc ggatcacctg cgtggggtcg tccggcaaca cgatgtcacc cccgtcatag
    10261 ccatgatgca cgatcggatt gaccccctga ccactacagt caggggcagt gtccatatgg
    10321 gcgcaaaaac aaacgacagg cacctgttta tccgtattgg acgggagggt tgcataaaca
    10381 tagccgaact catccagctg cgcgtcgtcc agaccggccg accgtaattc ctccaccagg
    10441 aggcggccaa gatctttttg cttttcagtg gaagggcagg tcttcgagcc gggatcgctt
    10501 tgggtatcga tccgggcata ccggataaat ctttccgcta cggtaaaagc atagttttcg
    10561 aacatgttcg taaattacca attaatatac catctatgac ggaagcgatc gtaaaaaaac
    10621 cggtgattcg gcaaggctgg ttgagagtcc tgatatttgg cgccctcttt ataattctca
    10681 ctttgctgat agccatacct gccgccatgc tggtagccgg tatccccgga gatcagctga
    10741 aacaacaacc gatcgccacc ttatcgggac tgctgacggg taattatctc tggttgatga
    10801 tccttgtcga gctggcgatc tcggtgatca gcgtcggtgt ctgccggtta tggctcgatc
    10861 gccggccgct ggttgactcg ggctggttcg cggatggctt cacaggtgag gccatcaccg
    10921 gtattttcat gggacccgcg ctgctgggca tcgtcgcgat cgtgttgctg ctcaccggtc
    10981 atgtggaatg gacggatgtc gtgtgggcgc cttcgtcatt attcgtttcg ctcggactaa
    11041 tggcactgat cgcttttagc gaagagctgg tattccgggg ctacgtcctc ggcaatctcc
    11101 tggagaccct tcctaataaa tgggtagcgc tgatcatcgc ttcggccctt ttcgccggct
    11161 tccattttcc caatcccagc atctatgccc tggccttcgt caatcttttc ctggccggtc
    11221 tgctgctggg tatcaactac atatatacga agaatctctg gttctccttc tttttccatc
    11281 tgagctggaa ctatttcgcg ggcccgatca tgggtttcca tgtcagcggg ttggcgcagc
    11341 cttcgctgtt gcaagccgac acgaagggcg acctgctgat caccggcggc gatttcggac
    11401 tggaaggttc catcctgacc acctgtgtgg ccgtgatcgc attttttgtg ctgttatggg
    11461 cttttgaaag aaaatatgcg tctgctcccg cgccgcctct cactccttcg acgcaattgt
    11521 agccgccctt gcggccgcta cggcatgatg atacgagaag ggctcttgat atcgacgagc
    11581 tcgagatcaa agatcagatc ctgccctgcc agctgatgat tggcatcgag caccactact
    11641 tccggcttga cctcgacgat caccaccggt atctgctgtc cggcaccgtt attcatgaac
    11701 agttgcattc ctacttccgg cttcatctcg gccggaaacc gctcgatcgg aaattcgatg
    11761 atcgcttccg ggtccctcgg gccataggca tcttcaacgg ggatctcgat cgtctttttc
    11821 tgtcccacag tcattcccat aaccccttca tcgaatcctc tgatcaccat accaccgccc
    11881 acctcgaatt ccagcggctg acgcccttcg gacgaatcga aggtcgttcc gtccgtaagt
    11941 cggccatggt agtggacctt gacagtatct cctgctttta cttcctgtgc catagtaaaa
    12001 ttttatttgc ccgtgacttc cggacagtct gcaaggtagc attaaataat taaattgtgc
    12061 ccaaatccgg tcatcgtacc gctaaagaaa caactatgcg aaaggcactt atcgtcatcc
    12121 tgatcatcct gttcatacaa ccgattaccc gcgcacagat cgtgattggc gccgaacaaa
    12181 tggatcggta cctccccttg ctgaaaggcc ggtccgtggc cattttcgcc aatgctacct
    12241 ccgtagttgg caacggcaac acccacctcg tggacacctt gttgaagaaa ggtatccata
    12301 tcgttaagct cttttctccg gaacatggtt tccgcggcga tgccgacgcc ggcgcgaaag
    12361 tgggcaacca ggtggactca aagacgggta tacccatcat ttctttatat ggcgccaaga
    12421 ccaagcccac agccgaagat ctcgcagacg tagacatcat gctctacgat atccaggatg
    12481 tcggcgtgcg attctacacc tatattggat cgctccagcg ctatatggag gcggccatcg
    12541 aaaaccatcg cccgttggtg atactcgaca ggcctgatcc caacggctct tacgtggatg
    12601 ggcccgtact cgacaccgcc taccggtcgt tcgtcggcat gcaacccgta ccgatcgtct
    12661 atggaatgac catcggcgaa tatgcgcgaa tgctcatcgg cgaacattgg gtacatgccg
    12721 atcccggctt caagcttacg atcataccgt gccggaacta tactcaccgg agcaagtata
    12781 tccttccggt aaaaccatcg cccaatctcc ccactatgca atccgtttac ctctatggtt
    12841 cgttgtgtct cttcgaaggc accgacgtca gcctgggacg cggaactacc aaacccttcc
    12901 agcaattcgg caaccctgat tttccgaaag acctctacag ctttaccccc gaaagcatgc
    12961 cgggcgctac caaccctcca cagttgggca agacctgcta cggttatgat ctcagcgctg
    13021 tttcgatacc agccaccggt ctttcattcc aatgggtgct gcgcgcctat tcgctgtccc
    13081 cgaacaagga ttctttcttt ctcaggaatg gccgggggtt cgaccgactt gcgggtggac
    13141 cggttctccg gcagcagata cgggacgggt tgtccgaagc ggatatccgt aaatcatggg
    13201 agcctgcatt gcagcaattc aaggcgatcc ggaaaaaata cctgctctat gccgactgac
    13261 ccgcgcatcg tattcatggg cacgcctgaa ttcgccgtcg ccagtctcga tgccattctt
    13321 gcggccggat atcgtgtcgt tgccgtggta accgccccgg ataaaccggc aggtcgcgga
    13381 atgcagttgt cggtcagcgc cgtaaaaaaa tatgcgcttg aaaaaggtct gcacttactg
    13441 cagcccgaaa aattgaaaaa ccccgctttc ctcgaggact tgcgtgcctt gcaagccgaa
    13501 ctccagatcg tcgtggcctt ccgcatgttg ccggaagccg tttggaacat gccgccgatg
    13561 gggactgtca atctccatgg atcgctgctg ccgcaatatc gtggcgccgc gcccattaac
    13621 tgggcagtca tcaacggcga acccgtcaca ggtgtgacga ccttcaggct gaagcatgcc
    13681 atcgacacgg gcaatatcct tttgcaggag agatttccta tcagcgagga ccagacggcc
    13741 ggagaagtgc atgataagat gaagatgatc ggcgccgggg tgctggttcg tacgatcgac
    13801 ggtctcgttg acggcagcct ccacgagaca ccgcaggaaa acccacccga tctgaaaccc
    13861 gctccgaaga tcgtcaccga aacctgccgg atcgattgga ataaacctgc gcgtgaagtc
    13921 cataatctga tccgtggcct ttctccattt cccacggcat ttaccactct cgacgggcgg
    13981 atgcttaaga tttaccgatc ggaaattgaa ataaagcctc ccgccgtggc cccgggaatc
    14041 catgacaccg acggcaagac ctattttcgc tgggccgcct ccgacgggta tatccgggcg
    14101 accgaggtgc aactggaagg gaaaaagaag atggccattg aagattttct gaggggctac
    14161 cgctaataga tgacctgcac ggcaaatttt ccctcaccct gcccaagctc cgcggccgcg
    14221 gcattgctga tgcgaatggt aagcccggca ctttctttca tatccggaag ctgccccagc
    14281 accttcgtaa agacgctttt tcctgtggca ggatcggtaa ccttcacgat ggtcccgaca
    14341 ttcacattat tcatgagcgc atagtatttc ccatcctgcc atccgctggt gctcttgaac
    14401 acgcccgcct gaccattgac cgaatggccc tcatcgctga aatctccacg gaatacgcct
    14461 ccatcgaaat gaggagcatt ggggatcact gccggaggtt tgaccgtagt ggttgtttct
    14521 tgcgccggca ccggagacgt agcctgcgga tgcacccggg tgcccgcagg cgaatcggtc
    14581 ttcacgggcg tggaagccgc atcattgccg gtagacaagg cctggtgcac caccggtgac
    14641 acaggaggcg gtgtagcggc agcaaccgga gcggaaacag aagtcgcgag tgccgacagg
    14701 gccgtcttta ccttcagata gcctacgata agatgcaaac ccggatgcag ctggtcgccg
    14761 ttgatatgat tccatttttc aagagtcgga ataggaacct tgttgtgatt gacgctgatg
    14821 cggtacatcc attctttctc cctgacgata tggaatacgg ggacaagggt ctccgaggcc
    14881 gtctttacgc catcttgcga gaaattagtt gtcgtgagag gtatttccag ggtctggccg
    14941 atggtcaggg gttgttgcag cgtgtgatga ttgtacggag ccagcgtttt ggggctgaca
    15001 ttatagagac gtccgacact gtaccagttt tctttcgcca ccacggtatg ctccaggtat
    15061 aattttccgg tctgtccctg aatgagcagc tcgccggact ggccgtgcgc gctgtctgaa
    15121 aaaaatacag tgagtagtaa aagtacggcc gccggtattc gcatagtatg gaagtttctg
    15181 agcaatgtta ctgatttttc acgtcctttt caatatccgc cattgtttgg ggtaatagtt
    15241 gttccagcgg ctgggcagaa ctttcaccca accgagatta tggccggcat accggaccag
    15301 tgaccacccg cgatgttctg tttccgtcct tacctcttct tttcgcagat agttcagcgc
    15361 ctgttcatgc gtcagcccga tggcaggcag atcaggatgg atcacggtgc tcatagccag
    15421 accgtggtca ggtatgaatt cctttgccga tggcgtcccc agcaccaccc cggctgtctt
    15481 cagatagcac gcagactgaa gaatgggtag ctccgctgcc aacggctcag gtaacacatg
    15541 tatctgatcc ccatgatgga aaaaaaataa tgccgtctcc ggcttcaccc atttatggag
    15601 tgactccagc tcttttttgc ctgcacgttc cggttgcgct gttcgccgcg gcgggctgaa
    15661 tgttcctccg ccattcttgc gcagcgcacc gataaaaaat ccttcgccat tgagccggtc
    15721 ggggtaaaaa cgatagcccc aggtcttttg aatgccctcg ctctccacga tattccattc
    15781 cggcgaaaga agaagacggc aaggggtcgc atcaaactct tccgccgtcc aatcgaggat
    15841 atcctcgttt tcctcccggg aatacgaaca ggtggaatag ataaggacgc cgtcctggcg
    15901 aagcgccggc catatatcgg acaggatacg ctgctgccgt ccgctgcaca attgcacctg
    15961 ggcggggctc cattctttca tcgtctccgg gtcgcgacga aacaatccac tgccgctgca
    16021 tggcgcatcg acgacgatca cgtcgaaata gttttcgagt ctggaaaggt ctcttggatc
    16081 gttgttggtg actaccgaat tcacgcctcc ccatttgatc atattttcct cgaggacggg
    16141 gaccctgctc ttgatgacct cgttgctcac gagcagactg ccggccgcca atgccgcctg
    16201 caacagcgta gatttacctc ccggcgcggc gcagagatcg agcactcgta gcggtctggt
    16261 gacatcggcc gtctgacgca acgcctgttc gaggaacatg ctcgatgctt cctgcacata
    16321 atacgccccg gcatgtaata gcggatcgaa tgtgaaatag gggcgcacgg ccaaataata
    16381 gccccatgag gaccagggga cacgggttgc ccccgcagga cccgaatccc ctgtcttgtc
    16441 agggttgacg cgaatcgagg tgacctgtct gtcgccggca tgcactgcct cgaaggctga
    16501 ggcatcatac cccggaaggc cacggaggga ggcgaggaat tgttttgata aaagggagga
    16561 cactcctgca aaattattgc aattttgcgt gcttttcaag gtaaaacatc gtccgggttg
    16621 tgccgaacca ttactcaaaa cctaattgta aaatcctgtt tcaccctgaa ctcctcccgg
    16681 ataaaaacaa agcccagaaa aaacagatcg atcgtcagca ttacccgcgg atcgcggcgg
    16741 atatcctccc acgcgcgttc catttcgccg ctccaataga tatcatcgaa tatcagcacg
    16801 gcgcccggag atgcatgcgc gaaaagactt tcgaaataac gcatggtcgg ttcataccgg
    16861 tggttgccgt cgataaaggc gaggtctacc ggcggaatgg tttgcaacaa cggcgcaagc
    16921 tcgtcgtcga agtttcccgt cacgatccgt gcatcgagac ccaatgaccg caggttgccg
    16981 gcggccgctt ccgcaagggc ggcagagcct tctatggtgt ataaacgcga tcccggtgct
    17041 ccggcttccc cttccgcccg atccaaacct gctcccgccg caagataagc cgtcgacaaa
    17101 cccagcgacg tgcctaattc gaccatcacg cccggccggt aatgccgtgc tatacgaaaa
    17161 agcaagcgtc ccagccgctg tggcttggcc gaacgcctga cgatgtccaa gaccctccgc
    17221 tgtgtacccg cccccgatac ggagcccgcg cccaaatcct cgatgggaag cacgccggta
    17281 tcccccgaca gtcgactccg caacatttct atcggcgcat attctgcata gatccgccga
    17341 tcattcaata catgcgtcac gaaatcgaac acgaaaggcg agtggacccc gtgccccttt
    17401 ccgtttgccg cggtaaggta atagcgaaaa tacttgcctg caagccgcag tggagaatac
    17461 atcaagctta ctttttccgt tcccataact gaaagctata ggcgaacgca tgtttatcgt
    17521 cgggttgaaa atccaggttg ccgctcatcg tccagtcctt ctcatcgata acggggaaaa
    17581 acgtatcccc ctccaaaacc gtatgtatcc tcgtcatgta tatcttgtcg gcgacagaca
    17641 tcgcttcggc atagatctgt ccgcccccga tgatgaacgc ctctttggcg tccgtctgtc
    17701 cggcctgatg caacgccgct gtcaggtcgt ggacgacctc gactttttca cgtcggggac
    17761 gccattcatt ctgacgggtg atcacgaaat tgaatcttcc aggcagcggt tcaccggcaa
    17821 gggcttcgaa ggtcttgcgg cccatgatca ccggcatgcc ccaggtagta tttttgaaga
    17881 atttcatgtc gtttggcaga tgccagagca atttgttatc cttaccgatg acgttattgg
    17941 tggtcgcggc gacgatgaga ctgatgatca tagcccaaat ttaatgcctc caccacaaaa
    18001 atttttacat aagagtgcta tgtatctaat aattatgtaa ctttaataga cgatggccct
    18061 attctacgct aaataattct tacatgtact cggaaaaaat cctcaacacc gcgtattcct
    18121 tcgcggccgc tgtcgtcgta ttcggggcct gggccaagct cgagcaaaaa gaattcggca
    18181 gcattgccct taccgtcgga ttattggttg aaagcggcat cttcatgatc tatgggctca
    18241 tggagtggcg cagccgcccg cgggccgcag gatcaccgga gcagcccgaa acgccgctgg
    18301 ccaaaatgtc ggaaaccccg acccctgaaa atgcacagga gctggcacgt accatggagc
    18361 gtaccaatcg aattctgacg aagatattcc gggcggaata gccttttata ccgccaccgg
    18421 cgcttttatg gcaggatggg aaatataatt ttcgagcgtg aagtcttcga accggaaggc
    18481 gaaaatatcc tttaccgcgg gattcagctt cattaccggc agcggatagg gtgtacgggt
    18541 cagctgaagc cttgcctgct cgatatgatt gctatagagg tgaacatccc cgaaggtgtg
    18601 tacgaattcg ccgggctcga gaccacacac ctgcgcgatc attagagtca gaagtgcgta
    18661 cgaggcaatg ttgaagggaa ccccgagaaa aacgtcggca cttcgctgat acagctggca
    18721 gctcagccgt tgccggccct ccccatcggg cgccacatag aattggaaaa ggacgtgaca
    18781 gggcatgagc gccatttccg gcagatcggt cacattccag gcgctgacga tcagacggcg
    18841 gctgtcggga gtcttcctga tctggtcgat cagctggctg atctgatcaa ccgttcttcc
    18901 gccggcccct tcccagctgc gccattgttt gccatagacg ggtcccagct ctccatcggc
    18961 atccgcccat tcgtcccaga tgctgacccc gtgttccttg aggaaccggg tattcgtctc
    19021 tccccgtaga aaccagagca gctcgtaaac gatactcttg agatgcagtt tcttggtagt
    19081 caccaggggg aacccgctgg tcagatcgaa ccgcagctga tgaccgaaac agctgagcgt
    19141 tccggtgccg gtacggtccg tcttccggac gcctttatcg agaatatgcc gaaggagatc
    19201 gaggtattgc tgcatgggtg caatttacgt attttcgtta tccctaaacc cccatacttg
    19261 cgactgctga gagtcaataa tttttccagc gagccctcgc ttcgaccggg aaggttcttc
    19321 ctcaccaccc gaataaaaaa accgggccgg ttcgatctta tcagctatct cgggatcctg
    19381 tctcaatccg gaaaaaccat tcttacccat cgtctttgcg ggatccccgg agatacgctc
    19441 gagctgaagg cgggcgagct atacgtcaac ggccaggaag ccgacggatc gctggctctc
    19501 aaaca
//
LOCUS       DASYSV010000400         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_336354, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000400 DASYSV010000000
VERSION     DASYSV010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336354"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VGQ51_12355"
     CDS             complement(<1..488)
                     /locus_tag="VGQ51_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037414.1"
                     /translation="MKHHFCLLIFFLFTALIASAQEKAAKKDSIAPKKSHWVAGLTYQ
                     NNDVYLGRRDSVAIPYLSPSFGYHDRSGFFITASASYLLTPGEQRFDAGSIEGGYSYE
                     SDHFSTEITAAKDFFSDQSFAVTSEILGRLSGHFSYDFDVIEPFVDIDANFSEKADMA
                     FG"
     gene            complement(610..1521)
                     /locus_tag="VGQ51_12360"
     CDS             complement(610..1521)
                     /locus_tag="VGQ51_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037415.1"
                     /translation="MKTKILPILLVFMTLYSCKKDPVTAPSNPALQQHNSAISPDSIV
                     NPAPDTTDLPVYPSTARSCNFLPIYGDTIIYPQPAAGGDDVLTPVNDPGAGHYFAWPA
                     GMVIDRNTGVINLTQSQTGLKYAIGFVKTGTTDTCLTYLTIGGASYVDSAYVVSGGGI
                     KALPYFNGNPFQTSNCVGSGQGSGCSFDVTGSAAALNVVVSNSSGEIDLQNTLLGGSG
                     QTGVFGATPFNGQTVTVPIYYQIRMGSNNALQHIDVQLMYFNSKADMNMALLSTLSTR
                     SMNLYGGGLISTSAMPRPPLIILVRKN"
     gene            1621..>2719
                     /locus_tag="VGQ51_12365"
     CDS             1621..>2719
                     /locus_tag="VGQ51_12365"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV9037416.1"
                     /translation="MDTITNWAGNLEYSTTALHSAASPEGIREWVRAQTGFKVLGTRH
                     CFNDIADSHDGFLSVAGMDGVVALDRSGRTVMVEAGIRYGELAVWLNDRGFALHNLAS
                     LPHISVAGGCATATHGSGIRNGNLSSAVVGMEFVTPEGETIRLTREHDGDLFRGALVH
                     LGGIGVITKLTLAVEPSYTMRQYVFGELPIAALEHHFDEIMSAGYSVSLFTGWRDRNI
                     DSVWVKVRSEDNADFAGRSSFFGAQAATVDVHPIRGISAEHCTPQLGVAGPWHERLPH
                     FRMGFTPSSGDELQSEYFVPCQHSFAAVMAVEALRERVAPHLLISELRCVAADDAWMS
                     PCSEGQVTAIHFTWKPDWKAVKQLLPLIEQAL"
BASE COUNT          618 a          647 c          812 g          642 t
ORIGIN      
        1 aatccgaacg ccatatcggc cttttccgag aaattggcat cgatgtccac aaagggctcg
       61 atgacatcga aatcgtagga gaagtgtccg gaaagcctgc ccagtatttc ggaggtgacg
      121 gcaaaactct gatcgctgaa aaaatcttta gcggccgtga tctccgtgct gaaatgatcg
      181 gactcatagc tatagccgcc ctcgatcgag cctgcatcga aacgttgctc accgggtgtg
      241 agcagatagg atgcactggc agtgatgaag aaaccggatc tgtcatgata gccgaatgac
      301 ggactgagat aagggatagc taccgagtcc cttctgccca ggtaaacatc gttattctga
      361 taggtgagac cggcaaccca atgtgatttt ttcggggcga tcgaatcttt tttggcggcc
      421 ttttcctgcg ctgaggcgat gagcgccgtg aataaaaaaa agatgagcaa acagaaatgg
      481 tgtttcataa ggttggtcga atttaaccat taacggagct ccggaccgtt tcgtacaagc
      541 gaaatgttat cggatcgttt ttatcttcgc aatcgggaca aatgctgtag agaacttgaa
      601 gaatctgtct taattctttc gaacgaggat gatcaacgga ggacgcggca ttgcagatgt
      661 gctgatcagt ccgccaccat aaagattcat cgaccgcgtg gacaaggtgc tcagcaacgc
      721 catattcatg tcggccttgc tgttgaaata catcagctgc acatcgatgt gctgaagggc
      781 attattgctt cccatcctga tctgatagta gatggggacc gtgacagttt gcccgttgaa
      841 aggagtggca ccgaagacgc cggtctggcc ggagccgccc agaagcgtgt tctgaagatc
      901 gatctctccg gaagagttac tgaccacgac attaagtgct gccgccgacc ctgtcacatc
      961 gaagctacag ccgctgccct gacccgagcc gacgcaattg gacgtttgga aggggttgcc
     1021 gttgaaataa ggcaatgcct ttattccgcc gccggagacg acataggcgc tgtccacata
     1081 ggaggcgccc ccgatcgtca aatatgtaag acaggtgtcg gtcgttcccg tcttcacaaa
     1141 accgatggcg tatttcaagc ctgtctggct ttgggtcagg ttgatcaccc ctgtattccg
     1201 atcgatgacc attcctgcgg gccatgcaaa atagtgtcct gcaccgggat cgttgacggg
     1261 tgtcagtaca tcgtcaccgc ccgccgccgg ttgggggtaa atgatcgtat ctccgtagat
     1321 gggaaggaaa ttgcaggatc ttgccgtgga cgggtagacc ggtaggtctg tggtgtccgg
     1381 ggcaggattt acgatactat cgggagaaat agcgctatta tgttgttgga gggcagggtt
     1441 cgacggcgcc gtgaccgggt cctttttaca ggaataaagg gtcatgaaga ccagcaggat
     1501 gggcaggatt ttcgttttca ttcggagggt attaggagtt actgctataa taaacggaaa
     1561 aatatcaccg gcggagggct gggatcgaaa aaattaatat atttaaaatc aatgtcttat
     1621 atggatacta tcaccaattg ggccgggaac ctggaatata gtacaacggc cctccattcg
     1681 gcggcatcgc ctgaggggat ccgggagtgg gtgcgggccc agacaggttt caaggtcctg
     1741 ggtacgcggc attgtttcaa cgatatcgcc gattcgcatg atgggttcct ttctgtcgcc
     1801 ggaatggatg gtgtcgtggc gctggatagg tccggtcgaa cggtgatggt agaagccggc
     1861 atcaggtatg gcgagctggc cgtctggctg aacgatcgcg gtttcgctct tcacaatctt
     1921 gcctcgctgc cacatatctc cgtggcaggc ggttgtgcaa cggcgacgca tggttcgggt
     1981 atacggaatg gtaatttgtc gagtgccgtc gtagggatgg agttcgtcac accggagggt
     2041 gagacgatcc ggctgacacg tgagcatgat ggcgatctgt tccgcggggc gctggtccat
     2101 ctcggcggca taggcgtgat cactaagctt actctggcgg tagagccatc ctatacgatg
     2161 aggcaatatg ttttcggcga gctgccgatc gcggcgctgg aacatcattt cgatgaaatc
     2221 atgtcggcgg gttatagcgt gagtcttttt acgggctggc gtgacaggaa tatcgacagc
     2281 gtatgggtca aggtccggtc ggaggataat gcagacttcg ccgggcgatc gtctttcttc
     2341 ggtgcgcagg cagctaccgt agacgtacat cctatccggg gtatctccgc ggagcactgt
     2401 actccgcagc tgggagtggc ggggccgtgg catgaacggt tgccacattt cagaatgggg
     2461 tttacgccca gcagcggcga tgagctgcaa tcggaatact tcgtgccatg ccaacattca
     2521 tttgccgccg tgatggccgt tgaggcctta cgtgaacggg tagcgccgca tctgctcatc
     2581 tccgagctgc gctgtgtcgc cgcggatgac gcatggatga gtccttgcag cgaggggcag
     2641 gtcacagcta tccattttac ctggaaaccg gattggaagg cggtgaaaca attattgccc
     2701 ctcatcgagc aggcgttat
//
LOCUS       DASYSV010000401         5678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1362159, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000401 DASYSV010000000
VERSION     DASYSV010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362159"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VGQ51_12370"
     CDS             <1..480
                     /locus_tag="VGQ51_12370"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV9037417.1"
                     /translation="YWLIRYYSWKVDLWAVTNFRVIDESGLLNHFAKESPLDKINNVS
                     YDQTVWGRIFNFGHVEIQTAAEVGATDYYNVNHPRRLKDTITAAQADYKSWQFGSQAA
                     QMAAAMDQRMDQRSGVAAQRAVAPQVAAELEKLFELKMKGAISDEEYERAKARLLNH"
     gene            complement(474..1499)
                     /locus_tag="VGQ51_12375"
     CDS             complement(474..1499)
                     /locus_tag="VGQ51_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037418.1"
                     /translation="MVSLSFCLFLTGLSLLSAQTKAQQLTAATAQQLTAAKVPQLTPA
                     QIHEFGVREDSMKGFADSIVNAESATQRFIADSQFIRRLVRALKEPNSFAYPFDSLGT
                     ISRLYSPDSAFRIFTWQFKKDNYIYLQEGAIQMNTPDGSLKLFPLFDASMFTDKPLDS
                     VRTRSNWIGAIYYRIILKTWQGHKYYTLLGFDGFSESSNKKWMEVLTFTPQGEPRFGG
                     PYFSFMEDSTHRHVQYRFDIQYKKEAATRFNYDPNMDMVVYDHLVPEGDHPEKKDTYV
                     PDGDFEAFQWKDGRWVHVTKELFSFRLKDGQFPMDAKLLDDQGKANQQALDEQSLKNL
                     QKKKPGQ"
     gene            complement(1575..3266)
                     /locus_tag="VGQ51_12380"
     CDS             complement(1575..3266)
                     /locus_tag="VGQ51_12380"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEV9037419.1"
                     /translation="MTTKGSVVIIGPAHPLRGGLATFDQRLAREFLDEGYDCVIYSFS
                     LQYPSFLFPGTTQYSTDPPPEGLTIFSRINSINPFNWWSVGEELRRRRPDIVVVRYWL
                     PLMGPALGTILRRVKKNRYTRIVAITDNVIPHEKRPGDKPFTRYFLRACDGFVTMSEK
                     VMRDLRTFQPDRPARQVNHPLYDNFGAPIPITDARKLLREKGVDIGDKDRVLLFFGFI
                     RQYKGLDIALRAMADPRVRNLGIRLLVAGEFYEDAAPYHQLIAELGLGDRLLLRTDFI
                     PDSEVREYVCAADAVIQPYRNATQSGVTPLAYHFEKPMIVSNVGGLPALVPHEKVGLV
                     AEPEPTSMAEAILRFFTLGTSYFHPGLQSEKQGYSWSRLVENLLLLGIPKMDDSWTLF
                     LDRDGVINVEKEGTYVLHYGEFVFYEGVKDALRICSQVFGRVVVVTNQRGVGKGMMTK
                     TDLQDIHNKMIAAIAETGGRIDRVYFEDSLDDDHPRRKPGPGMGHDAKRDLPDIDFSR
                     SVMAGNNLSDMEFGRSLGMYTVFIRTTNPEQPLPHPSIDLAFNSLYDFAKHLHRP"
     gene            complement(3263..4210)
                     /locus_tag="VGQ51_12385"
     CDS             complement(3263..4210)
                     /locus_tag="VGQ51_12385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9037420.1"
                     /translation="MELSIVIPVYNEDESLPELCSWIERVVREHHFSYEIILVDDGST
                     DRSWEVITTLRQANPHIKGIKFQRNYGKSAALNEGFKAAHGDVVITMDADMQDSPDEI
                     PELRRMILEDRYDLVSGWKKVRHDPISKTLPSKFFNWTTSKVSKIRLHDFNCGLKAYR
                     NKVVKSIEVYGEMHRYIPVIAKWSGFRKIGEKVVQHRARKYGTTKFGGLGRGLKGLLD
                     LASITFVGKYGKRPMHFFGPWGVIFFLIGLGETISLVIQKLDNRTSFTLTNKPSFYIA
                     LTCTLLGAQLFLTGFIAELISRNSPGRNFYLIEQKLGLE"
     gene            complement(4220..4792)
                     /locus_tag="VGQ51_12390"
     CDS             complement(4220..4792)
                     /locus_tag="VGQ51_12390"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEV9037421.1"
                     /translation="MKPQITPGLTSQTNAGKKNPVPLIFGGLASLGMILFTFCTYKAG
                     TRAFLSSSVFLMYLIPIGCGIIAAIIVRIRNGGFLEFRAALRIIFGIMVVSMALQTVF
                     TWLLVHVIDPGFGRELGPVVIAKMEAAWRRFKMPEDDIARNIAAAKGEDNFRFGNMFL
                     GLARDYVIGFLISLLLAALVKRKNPAGGKV"
     gene            complement(4789..>5678)
                     /locus_tag="VGQ51_12395"
     CDS             complement(4789..>5678)
                     /locus_tag="VGQ51_12395"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV9037422.1"
                     /translation="LEGKDLAEMYDMRNSGAIAFSDGTTPVQSAGLLLKGLQYIKAFN
                     GVIIQLPDDRTVGANGLINEGIVSTRLGLPGKPMMAEELMVARDIELTRYTGSRIHFT
                     GISSPRSLEYIRAAKAEGLSVSCSVTPHHLFFTDADLLDYDTNLKVYPPLRDAQAVDA
                     LRTAVLEGLIDAIASHHFPQEFDGKTVEFEYARPGMSSLETVYPVLRTRLQELTPERT
                     VDLLSNGPRRLFGLEQISLKEGLEASLTFFDPAGKTLLQTGDTKSRSKNNPFIGRTLE
                     GRVIGTLTGGRLHLNQYQG"
BASE COUNT         1292 a         1504 c         1578 g         1304 t
ORIGIN      
        1 tactggctga tccgttatta ctcctggaag gtcgatctct gggcagtcac gaatttcagg
       61 gtcatagacg agtcgggatt gctgaatcac tttgccaagg aatctcctct ggacaagatc
      121 aacaatgtct cctatgacca aacggtatgg ggtcgtatct tcaatttcgg gcatgtagag
      181 atccagactg ccgcggaagt cggcgccacg gactattata acgtcaatca ccccagacgg
      241 ctaaaggata ccatcaccgc cgcgcaggcc gactataaaa gctggcaatt cggatcgcag
      301 gcggcccaga tggcggcggc aatggatcaa aggatggatc aacgcagcgg cgtcgccgcg
      361 caacgggcgg tggcgccaca agtggctgcc gagctggaaa aattattcga actgaaaatg
      421 aaaggcgcca tctcggacga agagtatgaa agggccaagg cgcgtctcct caatcactga
      481 cccggtttct ttttctgcaa attttttaga gactgttcat ccaacgcctg ctgattggcc
      541 ttaccctgat catcgagaag cttggcgtcc atggggaatt gtccatcttt cagcctgaag
      601 ctgaatagct ccttcgtgac atggacccaa cgtccatcct tccattggaa cgcttcgaaa
      661 tcgccgtcgg ggacataggt gtcctttttt tcgggatggt caccttccgg gacaaggtga
      721 tcatagacga ccatgtccat attcggatcg taattgaacc gggtagcagc ctctttcttg
      781 tattggatat cgaagcggta ttgcacgtgt ctatgcgtgg aatcctccat gaaggagaag
      841 tatgggccgc cgaaacgggg ctcgccctgc ggggtgaaag tgagcacttc catccatttc
      901 ttgttgctgg attcgctgaa gccgtcaaaa cccaataggg tatagtattt atgcccctgc
      961 caggttttca ggatgatgcg atagtagatt gcgccgatcc agttcgatct cgtcctaacg
     1021 gaatccagcg gcttgtcggt gaacatcgac gcatcgaaaa gaggaaacaa tttcagcgac
     1081 ccgtcgggcg tattcatctg aatggcgccc tcctgcaggt agatataatt atcttttttg
     1141 aattgccagg tgaagatgcg aaacgcactg tcaggcgaat aaagacgcga gatcgtcccc
     1201 agggaatcga aggggtatgc gaaggaatta ggctctttca gcgctcttac cagtctccgg
     1261 atgaactggc tgtccgcgat aaaacgctgt gtcgcggact cggcgttgac gatactatcg
     1321 gcaaatcctt tcatcgagtc ttcgcgaacg ccgaattcat ggatctgcgc gggcgtcaat
     1381 tgcggaactt ttgccgccgt caattgctga gcagtcgccg ccgtcaattg ctgagcctta
     1441 gtctgtgcgg acagcaggga cagtcccgtc aaaaacaggc aaaaagataa agagaccatg
     1501 cgaattcttc ggtttcctga acacatcatg ctgcaaagtt actaaggggc aaacggtaaa
     1561 acatggccaa aatttcacgg cctgtgcaaa tgttttgcaa aatcgtacag tgagttgaaa
     1621 gccaagtcaa tagaagggtg tggcaggggt tgttcggggt tggtggtccg gataaagacg
     1681 gtgtacatcc ccagcgaacg cccgaattcc atatcgctga gattgttgcc ggccatcacc
     1741 gatctggaga aatcgatgtc cggcagatcg cgtttggcgt catgacccat gccggggcct
     1801 ggttttcttc gtggatgatc gtcgtccagt gagtcctcaa aatacacgcg gtctatgcgg
     1861 ccgccggttt ctgcgattgc agcgatcatt ttattatgga tgtcctgcag atcggtcttt
     1921 gtcatcatcc ctttgcccac acctctctga ttggtgacga cgacgacgcg tccgaatacc
     1981 tgagagcaga tacgaagggc atcttttaca ccttcataga aaacgaactc gccataatga
     2041 aggacgtagg ttccttcctt ttcgacattg atgaccccgt cgcggtccag gaaaagggtc
     2101 caggagtcgt ccatcttcgg aatacccagc agaaggaggt tttccaccag ccggctccat
     2161 gaatagcctt gtttttcgct ttgaagaccc gggtggaaat aagaagtacc aagcgtgaag
     2221 aaacgcagga tcgcttcggc catggatgtc ggctccggct ctgcaacgag acccaccttt
     2281 tcatgcggga cgagagccgg aagcccgccc acattcgata cgatcattgg tttttcgaag
     2341 tgataggcga ggggggtaac cccgctttgc gtggcattgc ggtaaggctg gatcacggca
     2401 tcggcggcgc atacatattc gcggacttcg ctgtccggta tgaaatccgt tcgaagcaga
     2461 agccggtcgc ccagccccag ttcggcgatc agctgatggt aaggagccgc gtcctcatag
     2521 aattccccgg ctacgagtaa ccgaatgccg aggttcctga cccgcgggtc ggccatcgcc
     2581 cggagcgcga tatcgagccc tttatactgc cggataaatc cgaaaaagag caggacgcgg
     2641 tccttgtctc cgatgtcgac ccccttttcg cgcaaaagtt tcctggcatc ggtgatggga
     2701 atgggtgctc cgaaattgtc atacaacgga tgattcacct gtcgggctgg tctatcgggc
     2761 tgaaaggtgc gcagatcgcg catgactttt tcgctcatgg tgacgaatcc gtcacatgcc
     2821 ctgaggaaat atcgggtaaa cggcttgtcg ccggggcgtt tctcatgcgg tatgacattg
     2881 tcggtaatgg cgacgatgcg ggtataccgg ttctttttga cccgccggag gatcgtcccc
     2941 agcgcgggtc ccatgagcgg cagccagtaa cgaacgacta cgatatcggg ccggcggcgt
     3001 cgcagctctt cacccaccga ccaccagttg aaggggttga tggaattgat gcgggaaaag
     3061 atggtaagcc cctccggggg agggtcggta ctatattggg tggtaccggg aaaaagaaag
     3121 gaagggtatt gcagggaaaa agaatagatc acacaatcat atccctcgtc aagaaattct
     3181 ctggccaatc gttggtcgaa ggtagccagc ccgccgcgaa gcggatgtgc cggaccgatg
     3241 atgacgacgg agcctttggt ggtcattcca aacccagttt ttgttcgatg agatagaaat
     3301 tgcgccccgg cgaattccgc gagatgagct cggcgataaa cccggtaagg aacaactggg
     3361 ctcccagcaa ggtgcaggtc agcgcgatat agaaggaggg tttgtttgtc agcgtaaagg
     3421 acgtacggtt gtccagtttc tgtatcacca gcgagatcgt ctcgccgagc ccgatgagaa
     3481 aaaagatgac gccccaggga ccgaaaaaat gcatcgggcg tttgccatat tttccgacaa
     3541 acgtaataga agccaggtcc agcaggccct tcagacccct gcccagaccg ccgaatttgg
     3601 tggttccgta tttgcgcgcg cggtgctgca ccactttttc accgatcttc ctgaaccccg
     3661 accattttgc gatcacgggg atataacggt gcatctcccc gtaaacttct atactcttga
     3721 ccaccttatt ccgataggcc ttcagtccgc agttgaaatc gtgcagccgg atcttgctga
     3781 ccttcgaagt cgtccaattg aagaatttgg aaggaagggt tttggagatg ggatcgtgcc
     3841 ggactttttt ccaaccgctg acgagatcgt agcggtcctc gaggatcatg cgtcgaagtt
     3901 cggggatttc atcggggcta tcctgcatgt cggcatccat cgtaatgacg acgtcgccat
     3961 gtgccgcctt gaagccttca ttaagtgccg cggacttccc gtagttgcgt tggaatttta
     4021 tccctttgat atgagggttt gcctgtcgca acgtggtgat cacttcccag ctgcggtcgg
     4081 tgcttccatc gtccacgagg attatctcgt aggaaaagtg atgctcgcgc acgacgcgtt
     4141 cgatccagct gcaaagttcg ggcagcgatt catcctcatt gtagacgggt atgacgatcg
     4201 atagttccat gatggttgat tatactttcc ctcctgcggg gtttttgcgt ttgacaagcg
     4261 ctgcaaggag cagcgagatg agaaaaccga tcacatagtc ccgggccagt cccagaaaca
     4321 tgttgccaaa tctaaagtta tcttcgcctt tggccgcggc aatgttcctt gcgatgtcgt
     4381 cctccggcat tttgaacctc cgccacgcgg cttccatctt cgctatcacg acgggcccca
     4441 gttccctgcc aaaacccgga tcgatgacat gaaccaacag ccaggtgaat accgtttgca
     4501 gcgccatgga cactaccatt atcccgaaaa tgatccgaag agcggcccgg aattccagga
     4561 aacccccgtt ccggatcctc acgatgatgg ctgcgatgat cccgcatcct atcgggatca
     4621 ggtacatgag aaagaccgag gaactcaaaa aagcccgggt tccggcctta taggtgcaaa
     4681 aagtgaataa gatcatgccc agagaggcaa gccccccaaa aattagcggg accggattct
     4741 ttttcccagc attcgtttgc gaagtcagcc ctggagtaat ctgcggcttc atccttgata
     4801 ttggtttaga tgaagacgtc cgccggtaag ggtaccgatg acgcggcctt ccagggttcg
     4861 cccgatgaac gggttgttct tcgaacgcga cttcgtatcc cctgtctgca ggagggtctt
     4921 acccgcgggg tcgaaaaacg tcagcgacgc ttccaggcct tcctttagcg agatctgctc
     4981 cagtccgaac agccggcggg ggccgttgct gagcagatcg accgtccgtt cgggcgttag
     5041 ctcctgcagg cgggtccgca atacggggta tacggtttca agactgctca ttcccggccg
     5101 cgcatattcg aactccaccg tcttgccatc gaactcctgc gggaaatggt gagaggcgat
     5161 cgcatcgatc aggccttcca ggacggccgt cctcagcgcg tccacagcct gtgcgtcccg
     5221 cagcggcgga tatactttca ggttagtatc gtaatccagg aggtcggcat cggtgaagaa
     5281 caggtggtgg ggtgtcaccg aacagcttac gctgagtccc tccgctttcg ccgcgcggat
     5341 atattcgagc gaacgtggtg agctgatccc ggtgaaatgg atcctcgatc ccgtataccg
     5401 cgtcagttcg atatcgcggg cgaccatcag ctcttcggcc atcatcggct taccgggaag
     5461 gccgagacgg gtcgatacga taccctcatt gatcaggccg ttggcgccga ctgtgcgatc
     5521 gtcgggcaat tggatgatga cgccattgaa agccttgatg tattgaagcc ctttcagcag
     5581 gagacccgcc gactgtacgg gagtggtacc gtcgctgaag gcaatggcgc cgctattgcg
     5641 catgtcatac atctccgcca ggtccttccc ctcgagac
//
LOCUS       DASYSV010000402         2751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2513101, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000402 DASYSV010000000
VERSION     DASYSV010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2513101"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGQ51_12400"
     CDS             <1..244
                     /locus_tag="VGQ51_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037423.1"
                     /translation="EEYPFNLMRTRQFGYYLPEWVESYKEVTIGKIADLGSHMLLSGQ
                     WTEEVLLKPSTPHLHHIHFLHFLYLKATGKTYPVIQ"
     gene            complement(234..1466)
                     /locus_tag="VGQ51_12405"
     CDS             complement(234..1466)
                     /locus_tag="VGQ51_12405"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9037424.1"
                     /translation="MRLNDLRVPGQEGTIDLVIGDGNIVSVSPGGEAASRERSKTRDL
                     AATRDLAATRDHANPSGQEGIPFDGAIVFAGLINSHDHLDFNLFPLLGNRVYTNYTQW
                     GSDIHANQSSVIREVLKVPHRLRIAWGIYKNLLNGFTTVVNHGTLLPVEMDLIDVFQQ
                     CHCLHSVGFESNWKWKLNRPLAGWRPFVLHVGEGTDVPASEEIDRLIHWNLFRRPLIG
                     IHGVAMNEEQAASFRALVWCPASNYFLLNKTAPVARLKDRVPILFGTDSTLTGSWNGW
                     QQIRQAREEKALTDQELLATLTTTPARIWRLHDRGEIAPGKLADLVIARPKPGLRGMD
                     AFFGVDPEDLLLVLRRGRPLLFDARLRQALTDGGLEAARYKSIASGSKYVLGDLAGLI
                     AEIRSYFPQAEFPRWITE"
     gene            complement(1453..2178)
                     /locus_tag="VGQ51_12410"
     CDS             complement(1453..2178)
                     /locus_tag="VGQ51_12410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV9037425.1"
                     /translation="MQSVRPSRLPEAAPADAYDIWAQSYDNQPDNLMLALDEQLCGQL
                     LDKVLSPGKLIADVGCGTGRHWQKLYDLEPSRLVGYDVSSGMLDRLRAKYPRAETYLV
                     NGVHLSQLDTAGCDLILSTLTIAHIPDLRAALTEWIRVLRPGGDMIITDYHPAALSRG
                     GQRTFREGQHVIAVRNHIYPIGKIRRICRQLGLREQNFIERKIDDTMRHYYEKQGALP
                     LFERFAGVPIIYGLHLRKSDAAE"
     gene            2265..>2751
                     /locus_tag="VGQ51_12415"
     CDS             2265..>2751
                     /locus_tag="VGQ51_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV9037426.1"
                     /translation="MNKILLFNPRAAINKFRVPNSILQVAASVEGLFEWAIVDGNREE
                     DPYEKLKGYLSTGEYKYLGFTVMPGPQTKQAIPFAKRIKEEFPDTVMIWGGYFPNSHY
                     KVILASGYVDFVINGPGDHAFPQLVTALENNEPFQGIKNLIYRVEGKVIRTPKEELID
                     QG"
BASE COUNT          637 a          763 c          733 g          618 t
ORIGIN      
        1 cgaagagtat cctttcaacc tcatgcgtac ccgtcaattc gggtattact tgcccgaatg
       61 ggtggaaagc tataaagagg tcaccattgg aaaaatagcc gacctcggca gtcatatgtt
      121 gctaagcggg cagtggaccg aagaagtcct gctgaaacct tccacccccc atcttcacca
      181 tatccatttc ctgcatttcc tctatttgaa agccaccgga aagacctacc cggtcattca
      241 gtgatccagc gggggaattc cgcctgggga aaataggatc gtatttccgc gatgaggcca
      301 gcaagatcgc caaggacata tttactgccg gacgcgatgc ttttgtatcg agcggcctcc
      361 aggccaccat cggtcaaagc ctgccggaga cgggcatcga ataacagcgg ccggccccgc
      421 cgcaacacca gcagcaggtc ttccggatcc acaccgaaaa aggcgtccat gccacgcaac
      481 cccggcttcg gcctcgcaat gaccaggtcg gccagtttcc ccggggcgat ctcccctctg
      541 tcgtgcaggc gccatatcct cgccggtgtc gtagtcagcg ttgccagcaa ttcctggtct
      601 gtaagagcct tctcctccct ggcctgcctt atctgctgcc agccattcca ggagcctgtc
      661 agggtgctgt cggtgccgaa aaggatgggc accctgtctt tcaaacgggc caccggcgcg
      721 gtcttgttca gtaaaaaata attggaggca ggacaccaga cgagggctct gaaggatgcg
      781 gcttgctcct cattcatcgc gaccccgtgt atcccgatga ggggccgtcg gaagaggttc
      841 cagtggataa gccggtcgat ctcctccgat gccggcacgt cggttccctc cccgacatgt
      901 agtacaaacg gccgccatcc cgccagcgga cggttcagct tccatttcca attgctctcg
      961 aaacccaccg agtgcagaca atggcattgc tggaaaacat cgatcagatc catttccacg
     1021 ggcaggagag tcccatgatt gaccaccgtg gtaaacccat tcaaaaggtt tttgtatata
     1081 ccccaggcta ttcgcagtcg gtgcggtact ttcagcactt cccggatgac cgatgattga
     1141 ttggcgtgta tatcggaacc ccattgtgtg taattggtat agaccctgtt gcccagaagg
     1201 ggaaagagat tgaagtccag atggtcatgc gaattgatga gacccgcgaa gacaatggcg
     1261 ccgtcaaagg ggatgccctc ttgtccggat gggttcgcat gatcgcgtgt ggccgcaaga
     1321 tcgcgtgtgg ccgcaagatc gcgggttttc gagcgttccc gggaggccgc ctctcctccg
     1381 ggcgatacgg acacgatgtt cccgtcgccg atgacgagat cgatcgtccc ttcctgtccg
     1441 ggcacgcgga gatcattcag ccgcatccga ctttcttaga tgaagaccat agatgatagg
     1501 aacgccggcg aaacgctcga ataacggcag ggccccctgt ttttcataat aatgccgcat
     1561 cgtatcgtcg atctttcttt cgataaaatt ttgctccctg agccccaatt gccggcatat
     1621 ccggcgtatc tttccgatag ggtagatatg attgcggacg gctatgacat gttgtccttc
     1681 gcggaaggtg cgttgcccgc cccttgacaa tgccgcagga tgatagtctg taatgatcat
     1741 atcccctccg ggtctgagca ctcttatcca ttcggtcaga gccgccctca gatcggggat
     1801 atgggcgatg gtaagggtgg ataggatcag gtcgcaacct gccgtgtcca gctgggatag
     1861 atgcactcca ttaaccagat aggtttccgc ccgggggtat ttggcacgca atctgtcgag
     1921 cataccggag gatacatcgt aacccaccag ccgcgacggt tccagatcgt aaagtttctg
     1981 ccagtgccgg cccgttccac agccgacatc ggcaatcagc ttcccggggg agagcacctt
     2041 gtccaacaac tgcccgcaga gctgctcgtc gagggcaagc attaaattat ctggctgatt
     2101 atcatagctt tgtgcccaga tatcgtaggc gtcggccgga gccgcttcgg gcagccggga
     2161 gggccggaca gactgcatta gtttatttaa aaaaggcatc ctttttccaa taacgagcgt
     2221 ttattgtttc cggatgcctt cattgctaaa tagtaaaaga atatatgaat aagatattgt
     2281 tgttcaatcc ccgtgccgca atcaataaat tcagagtacc caattctata ctgcaggtgg
     2341 ccgccagcgt ggaaggtctc ttcgaatggg cgatcgtgga cggcaatcgg gaggaggacc
     2401 cctatgagaa gctgaagggc tatctttcga ccggcgagta caaatacctc ggattcaccg
     2461 tcatgcccgg tccgcagacc aagcaggcta ttcctttcgc caaaaggata aaagaggaat
     2521 tccccgatac agtgatgatc tggggaggat attttcccaa tagtcattac aaagtgatcc
     2581 tcgcatcagg ttatgtagat ttcgtcatca acggtcccgg cgaccatgct tttcctcaac
     2641 tggtcacggc gctggagaac aatgagccat tccagggcat caaaaacctg atctacagag
     2701 tggaaggcaa agtgatcagg actccgaaag aagagctgat cgatcagggc c
//
LOCUS       DASYSV010000403         7124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1105655, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000403 DASYSV010000000
VERSION     DASYSV010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105655"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VGQ51_12420"
     CDS             <1..641
                     /locus_tag="VGQ51_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEV9037427.1"
                     /translation="IDAKGRYEGYSQPVTFPNYSAANGAANFIQLMTASEAWFLKAEA
                     GLRGWTGAGDPGTDYNAGITQSFAQYGLDATNYLNDATSTPAQYIDPNAVTAGQNDIK
                     TGSPYLSTVTIKWDNSATFQQKLERIITQKYIAMYPDGQEAWSEFRRTLYPKLYPNTQ
                     NYSNGTITTSKFIRRINFALSERNNNPAGVKDATTKLGGPDTGGTPLWWDVN"
     gene            643..3957
                     /locus_tag="VGQ51_12425"
     CDS             643..3957
                     /locus_tag="VGQ51_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEV9037428.1"
                     /translation="MNKTTISILILLGLSSCMEDRSKLFTRLPESRTGVNFRNLLKED
                     NEQFNVMQNPYFYNGAGVAVGDINNDGLPDLFFTGNMVKNRLFLNKGDFHFEDITEQA
                     GVAIKEGWCTGVTFVDINGDGLLDIYVCRAGLPNVAYRSNLLFINNGPPAPGTGGGRD
                     APTFTEEAAKYGLADIGYSTQASFFDYDNDGDLDLFVINESSPDYSKGLLDYSQLRGK
                     SGDSALHNHLYRNDGGHFTDVTTAAGISSNVLTFSLGLSTADIDEDGWPDIYIANDFN
                     ENDYLYINNHDGTFRERLRDKIDHTSQYGMGIDVADMNNDGLPDILELDMLPFDNHDL
                     KMHVGGDNFDKFQYLFGQGYGYQYMKNSLQRNNGDGTFSEIGQLAGIAATDWSWSPLI
                     ADFDNDGRNDIFISDGYKRDNTNIEFVKYSIDEAIKQQQGEPAVTIGEYVAKMKGIQP
                     GNFIYRNMGNDSFANKTAEWGIAAQTFSNGAVYADLDNDGYLDLIVNNMDDYAGIYRN
                     NGATLLKNNFLRIRLKGDAKNPFGVGAKVYGYGGGQVFYLEQLPVRGYQSSVDMTLHL
                     GLGRLPELDSLRIIWPDSRTQLLTHVRADQTMVLALKDTDGKWVYGGATGSRLLDTVS
                     GALDYVHRENPERDFTRQHMLNHYYSHNGPCMAKADVNGDGLEDLFIGGSKGNPGGLF
                     LQTRDHRWVRRAFPDLSADSLSEDVDAVFFDANGDGSPDLYVVSGGYEYSANSPSLQD
                     RLYLNDGKGRLHRAKDALPANTGAKSCVRVSDINGDGKPDLFVGGKVVPGKYPSSCAS
                     SIYINDGHGKFVDATDALNPELRHLGIVTDAVWVDVNGDKVKDLVVVGEWMPVRVFVN
                     QGGRLVDQSDKYVPFASSGWWNTVHAVDLDGDGLDDLVLGNYGLNSPLRASSAEPVQL
                     YTCDIDNNGIDDPIMTCYRDHVSYPFYPMDDILAQVPSLKKKFYDYEVYAKATIADVI
                     PSEKLQTITPLVANNFRTMYLHNTGRGFVPRDLPVEAQYSPVYAIASADVDHDGHPDL
                     VLCGNNMYNKILLGRDDANHGLVLLNDGKGNFRSASPARTGLTLRGDVRSIAVVGDRL
                     FFGVNDQRVRVFRIRN"
     gene            complement(3947..6919)
                     /locus_tag="VGQ51_12430"
     CDS             complement(3947..6919)
                     /locus_tag="VGQ51_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEV9037429.1"
                     /translation="MINHTRLLLALLSASLLAHAQEPAAIIPEPVSTQWHSGNFHLDA
                     GTVIEATDSDVLAANFFNDYLQRFYGMHLHVIKTSEPSSNAILLRTQPGQGPDGRYTL
                     ESTPAGITITGSTHSGTFYGIQTLIQLLPLSPFQYTPHGPRVREVHNGLDIPAVGILD
                     YPRFAYRGLHLDVCRHFFPVDFVKRYIDFIALHKMNYFHWHLTDDQGWRIEIKKYPRL
                     TEVGAWRSGTIIGHFPGTGNDSTEHGGYYTQDQIKEVVKYAADRYITVIPEIEMPGHA
                     SAALAAYPWLGCTGGPYHVQQTFGVFNDVFCAGNDSVFKFLEDVLDEVMPLFPCKYIH
                     VGGDECPKESWKHDPKCQARMKELGLKTEDQLQSYFIQRIEKYLNSKGKSIIGWDEIL
                     EGGIAPNATIMSWRGEDGGIDAAKQHHNVIMTPGKFVYFDHAQTHNDDSLTIGGYLPL
                     DTVYSYEPIPAALKGGDTSYVLGAQANVWTEYISNPRKVEYMIFPRLSALSEVLWSPK
                     ASRNYTDFQNRLATQYKRYALWRVDHYDVYFKPDPLVRNKIAEWQDRKFGLLMHWGTY
                     SQWGIVESWSICPEDEGWTQRRGPYSKSYYDYLHAYENLKTTFNPTNFNPGKWAAAAK
                     DAGMKYVVFTTKHHDGFCMFDTKQTDYKITSPQCPFSSNPKSNVAKEVFNAFRTDSFL
                     IGAYFSKPDWHSDDYWWSYFPPKDRNVNYDPARYPDRWNAYKDYTYKQIEELMTGYGR
                     MDLLWLDGGWVRPLSTVNAGVDWQRGIRYDQDVDMPKIAAMARSHQPGLIVVDRSVPG
                     PYENYTTPEQQIPTHPMDDPWETCMTMGDSWSYVPHDHYKSTTRLVQLLVKIVSRGGN
                     FLLNIGPSPEGDFDDTAYARLKEIGAWMKINGEGIYNSRPVFPWSSGNVYLTQAKDGS
                     ADYAFLLSENETRDIILPATVTIDRFTAKPKSIVTILGSKQRLHWKQEDKKMIIEIPA
                     KLQGKPVGEHALCFKISS"
BASE COUNT         1558 a         1702 c         2208 g         1656 t
ORIGIN      
        1 atatcgatgc caagggacgg tatgaagggt actctcaacc ggtaactttc ccgaactaca
       61 gcgccgccaa cggggctgcg aactttatcc agctgatgac ggcttcagaa gcctggttcc
      121 tgaaagcaga agcgggcctg aggggatgga caggggcggg agatccgggc accgattaca
      181 atgccgggat cacgcaatct tttgcgcaat acggtctgga cgctaccaat tatctgaatg
      241 acgccacgag cactcccgcg caatacatcg atcccaatgc cgtcacggcg ggccagaacg
      301 atatcaaaac cggatcgccg tatcttagta cggtcacgat aaaatgggat aattccgcta
      361 ccttccagca aaagctggag cggataatca cccagaaata tatcgcgatg taccccgacg
      421 gtcaggaggc ctggtcggaa ttccgtagga cgctgtatcc gaagctctat ccaaacacgc
      481 agaactacag caatggaacg atcaccacca gcaaattcat ccgtcggatc aatttcgcgc
      541 tgagcgaaag gaacaacaac cctgcgggcg ttaaggacgc gacgacgaaa ctgggcggac
      601 ccgacacggg cggaacgccg ctatggtggg acgtgaatta atatgaataa aacaactatc
      661 agcattctaa tccttttggg cctgtcttcc tgtatggagg acaggtccaa gttatttacg
      721 cggctacccg agtcgcggac gggggttaat ttccgcaacc tgctgaagga ggacaatgaa
      781 cagttcaacg tcatgcagaa cccatatttc tataatggcg cgggggtcgc cgtcggtgat
      841 atcaacaatg acggtcttcc cgatctgttc tttacgggta atatggtgaa gaaccggctt
      901 tttttgaaca agggcgattt ccatttcgaa gacatcacgg aacaggcggg tgtcgccatt
      961 aaggaaggat ggtgtaccgg tgtgaccttc gtcgatatca acggagacgg actgctggat
     1021 atttatgtct gccgggcggg tttgccgaat gtcgcctacc ggagcaatct gctgttcatc
     1081 aacaacgggc ccccggcgcc gggcaccggc ggcggacgtg atgcgccgac gtttaccgag
     1141 gaggccgcga aatatggttt ggcggatatc ggttattcca cgcaggcgtc cttttttgat
     1201 tatgataacg atggtgatct ggatctgttc gtgatcaatg agtcgtcgcc ggattattcg
     1261 aagggtctgc tggattactc gcagctacgc ggcaaatcgg gagacagcgc actacacaac
     1321 catctttacc gaaatgacgg cggtcatttt accgatgtga cgacggcggc ggggatttct
     1381 tcgaacgtgc tgacctttag tcttggcctc agtacagctg atatcgatga ggacggatgg
     1441 cccgatattt atatcgccaa cgatttcaac gagaacgatt acctctatat caacaatcac
     1501 gacgggacat ttagggagag gttgcgtgac aagatcgatc atacgtctca gtatggcatg
     1561 ggtatcgatg tggcggatat gaataatgac gggttgccgg atattttgga actggacatg
     1621 ctaccgttcg ataaccacga tctgaagatg catgtcgggg gcgataattt cgacaaattc
     1681 cagtacctgt tcggccaggg atacggatat cagtatatga agaacagcct gcagcgaaac
     1741 aacggggacg ggactttcag cgagatcggc cagctggcgg gcattgcggc caccgactgg
     1801 agctggagcc ctttaatagc cgacttcgac aatgacggcc ggaacgacat ctttatcagc
     1861 gatggataca aacgggacaa taccaatatc gagttcgtta aatattcgat cgatgaggcg
     1921 atcaaacagc aacagggtga gcccgcggtt accattggcg agtacgtggc caaaatgaag
     1981 ggcattcagc cgggtaattt catttaccgg aacatgggca atgacagttt tgccaacaaa
     2041 accgccgaat ggggtatcgc tgcacagacc ttctccaacg gcgccgtcta tgcggacctg
     2101 gacaatgacg ggtatctgga tctgatcgta aacaatatgg atgactatgc gggtatttac
     2161 agaaataatg gggcgacttt gttaaaaaac aattttttgc ggatccggct gaagggtgac
     2221 gctaagaatc cctttggcgt cggggccaaa gtctacgggt atggtggggg ccaggtgttc
     2281 tatttggaac agctgcccgt acggggttat cagtccagcg tggacatgac cctgcatctg
     2341 gggctgggca ggctacccga gctggactcg ctgcggatca tctggccgga ctcccggaca
     2401 cagttgttga cgcatgtacg ggcggatcaa acgatggtgc tggcgctgaa ggacacagac
     2461 gggaaatggg tctatggtgg tgcgacgggc tcgcgactgc tcgatacggt ttcgggcgcg
     2521 ttggattacg tacaccggga gaatcccgaa cgggatttta cccgacagca tatgctgaat
     2581 cattattatt cgcataatgg tccctgtatg gcgaaggcgg acgtgaacgg tgacggcctg
     2641 gaggacctct tcatcggtgg atcgaagggt aacccgggag ggctgttcct tcagacacgt
     2701 gatcaccgct gggtccggag agcgttcccg gatctgtcgg ccgactcgct gagcgaggat
     2761 gtggacgccg tctttttcga tgcgaatggg gatggtagtc ccgatctgta tgtcgtcagc
     2821 ggaggatatg aatattccgc gaattcgccg tcgctgcagg accggcttta tctgaatgat
     2881 ggcaaggggc ggcttcaccg cgcaaaggat gcgcttccgg ccaacaccgg cgcaaaaagc
     2941 tgtgtacggg taagcgatat caacggggat gggaaaccag atctgtttgt cggtggaaag
     3001 gtggttccgg gaaaatatcc ttcgagctgc gccagcagca tctatatcaa tgacggtcac
     3061 gggaaatttg tcgacgcgac ggatgcgttg aaccccgagc tgcgtcatct gggtatcgtg
     3121 accgatgcgg tctgggtgga tgtgaatggc gacaaggtga aggatctcgt ggtggtaggg
     3181 gaatggatgc cggtaagggt gtttgtcaat caggggggcc ggctggtaga ccagagtgac
     3241 aaatatgttc ctttcgcgag tagcgggtgg tggaatacgg tccatgctgt tgatctggac
     3301 ggggatggcc tggacgacct ggtattgggc aattatggat tgaactcgcc tttgcgcgca
     3361 tcgtccgccg aaccggtgca gctgtatacc tgcgatatcg ataacaacgg catcgatgac
     3421 ccgatcatga cgtgttaccg ggaccatgtg tcatatccct tttatccgat ggacgatatc
     3481 ctcgcgcagg tgccgtcgct gaagaagaag ttctatgatt atgaggtgta tgcgaaggct
     3541 acgatcgcgg acgtgatccc ttccgaaaag ctgcagacga tcacgccgct ggtggctaat
     3601 aatttccgga cgatgtatct gcataatacc ggacgcggat ttgtcccgcg ggacctgccg
     3661 gtcgaagcgc agtattcgcc cgtgtatgcg atcgcgtctg cggatgtcga tcatgacggg
     3721 catccggacc tcgtgctatg cggtaataat atgtacaata agatcttatt gggccgcgat
     3781 gacgccaatc atgggttggt gcttctgaat gacggcaagg gtaatttccg gagcgcatcg
     3841 cccgcgcgga cggggttgac gcttcgcggg gatgtccgga gtatcgcggt cgtcggagac
     3901 aggttatttt ttggtgtcaa cgatcagcgg gtgcgggtct ttcggatcag gaactgatct
     3961 taaagcatag cgcatgttct cccaccggct tgccttgcag ctttgccggt atttcgatga
     4021 tcattttttt gtcttcctgt ttccaatgga gacgctgttt gctgccgagg atcgtgacga
     4081 tgcttttcgg tttggccgtg aagcggtcga tggtgaccgt cgcgggcagg atgatgtcgc
     4141 gtgtctcgtt ttcgctcagc aggaacgcat aatcagccga accatctttt gcctgagtaa
     4201 gatatacatt accggatgac catggaaaga cgggtctgct attatagatg ccttcaccgt
     4261 tgatcttcat ccatgcgcct atctccttta gccgcgcata ggcggtgtca tcgaaatctc
     4321 cttcgggaga ggggccgata ttcagcagga aattaccgcc gcgcgagacg atctttacca
     4381 gcagctggac gaggcgcgtg gtcgatttat agtgatcgtg gggaacatag ctccatgaat
     4441 cgcccatggt catacaggtt tcccagggat cgtccatcgg atgagtgggt atctgctgtt
     4501 cgggggtagt atagttttcg tacggacccg gaacactgcg gtcgacgacg atgagaccgg
     4561 gttgatggct gcgcgccatg gcggcgatct tcggcatgtc gacatcctga tcgtagcgga
     4621 taccgcgttg ccagtcgaca ccagcgttga cggtagacag ggggcgcacc cagccgccgt
     4681 ccagccagag aaggtccatg cggccgtaac ccgtcatcag ctcttcgatc tgtttatagg
     4741 tgtagtcctt gtaggcgttc caccggtccg ggtagcgggc aggatcgtag ttgacattgc
     4801 gatccttcgg agggaaatac gaccaccaat agtcgtcgct gtgccagtcg ggtttcgaga
     4861 aatacgcgcc gatcaggaag ctatccgtgc ggaaagcatt gaagacctcc tttgccacat
     4921 tgcttttggg gttggaagaa aaggggcatt gcggagaggt gatcttataa tccgtttgtt
     4981 tagtgtcgaa catacagaac ccgtcgtggt gcttggtggt gaagactacg tatttcatgc
     5041 cggcgtcctt cgcggcggcg gcccattttc cggggttgaa attcgtcggg ttaaaggtag
     5101 tcttgaggtt ctcgtaggca tggaggtaat cataatagct tttggaatac gggccccggc
     5161 gttgcgtcca gccttcgtcc tcggggcaga tgctccagct ttccacgatg ccccattggc
     5221 tataggtgcc ccagtgcatc aggaggccga atttcctgtc ctgccattcg gcgatcttgt
     5281 tcctgacgag tggatcaggt ttgaaataaa catcgtaatg atcgacccgc cagagggcat
     5341 atcgcttgta ctgggtcgcg agacggtttt ggaaatcggt gtaattgcgg gaggctttgg
     5401 gactccacaa gacttcgctt agcgcgctca gccgggggaa gatcatgtat tccactttgc
     5461 ggggattgct gatatattcg gtccatacgt tggcctgtgc gccgagaaca taggaggtgt
     5521 cgccgccttt cagcgctgcc gggatgggtt cataggaata gaccgtatcc agcgggagat
     5581 agccgccgat cgtgagtgaa tcgtcgttat gcgtctgggc gtgatcgaaa tagacgaatt
     5641 tccccggagt catgatgacg ttgtgatgtt gtttggcagc gtctatgccg ccgtcttcac
     5701 cgcgccagct catgatggta gcgttggggg cgatgcctcc ttcgaggatc tcgtcccagc
     5761 cgatgatgct tttgcccttc gagttgagat atttttcgat gcgttggatg aagtagctct
     5821 gaagctgatc ttcggtcttc aggccgagct ctttcatacg cgcctggcat ttcgggtcgt
     5881 gtttccagct ttccttgggg cattcgtcac cgccgacatg gatgtatttg cagggaaaga
     5941 ggggcatgac ctcgtcgagg acgtcttcga ggaatttgaa aacgctgtcg ttgccggcgc
     6001 aaaaaacgtc attgaagacg ccaaaagtct gttggacatg atagggaccg cccgtgcagc
     6061 caagccaggg ataggccgcc agagcggcgg aggcatgacc cggcatttcg atctcgggga
     6121 tgacggtgat ataccggtcg gcggcatatt tcacgacttc tttgatctgg tcctgggtgt
     6181 agtaaccgcc gtgttcggtg ctgtcattgc ccgttccggg gaaatggccg atgatggttc
     6241 cgctgcgcca cgcgcccacc tccgtcagtc ttggatattt ctttatctcg atgcgccagc
     6301 cctggtcatc ggtcagatgc cagtggaagt agttcatttt atggagggcg ataaagtcga
     6361 tatagcgttt gacgaagtcc accgggaaga agtggcggca gacgtcgaga tggagaccgc
     6421 ggtaagcgaa ccggggatag tcgagtatgc cgacggccgg gatgtcgaga ccgttatgga
     6481 cctcgcggac acgcgggccg tggggggtgt attggaaggg gctcagcggt agcagctgga
     6541 tcagggtctg tatgccgtag aaggtgccgg aatgcgtgga gccggtgatc gtgatgccgg
     6601 cgggggtgga ttcgagggtg tagcgtccgt cggggccctg acccggctgg gtgcggagga
     6661 ggatggcgtt tgacgacggt tcgctggttt tgatcacgtg caggtgcata ccgtaaaacc
     6721 gctgaagata gtcgttgaag aaattggctg ccaggacatc ggagtcggtt gcttcgatga
     6781 cggtaccggc gtcgagatga aaattgccgg agtgccattg ggtgctgacg ggttcaggga
     6841 tgatggcggc gggttcctgc gcgtgtgcga gcaggctcgc gctgagcagc gcgagcagca
     6901 atcgggtgtg gttgatcatg cggcgaatat aagccggaat taccaaatgc cggcggagcc
     6961 ggctcgccca tagggtagat tttagcgaag caccggcaaa gtaattccac tttgatgggc
     7021 ggcgtcatgc cagatacgga tggtggcttt ttggaaggcg ttgctatcgc aggtcgggat
     7081 attcacgaag gtttgagggt tgcggtcgac cagcgggaac cagc
//
LOCUS       DASYSV010000404         5176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_337409, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000404 DASYSV010000000
VERSION     DASYSV010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337409"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..605
                     /locus_tag="VGQ51_12435"
     CDS             198..605
                     /locus_tag="VGQ51_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037430.1"
                     /translation="MKQHRPLRYWAWLFPVLLTAACKKDPYKMAAPRAINYILYTEKD
                     FSNDHDTIRFEILMRSGSKVLLDSPLAPMTIAQIPDSFHRIMFQKFVPSANQNDDLVV
                     GFLYQIDNVGMSWFLDSSKASGPSVKTVTYSFE"
     gene            complement(670..1434)
                     /locus_tag="VGQ51_12440"
     CDS             complement(670..1434)
                     /locus_tag="VGQ51_12440"
                     /inference="COORDINATES: protein motif:HMM:NF006693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV9037431.1"
                     /translation="MKKWDLQGKRALITGGTKGIGKAIAEEFLGLGADVMIVARDERE
                     VNRLVRLWQTERRMVTGVAGDVSRPECRQRIIEAVAERWGGLDVLVNNAGTNIRKRLV
                     QYAEEEYRSVLELNLFSIIELCRLCLPLLVHGHAASVVNIASVAGCVDVQTGAPYGMS
                     KAALIQLSRHLAVEWAEHGIRVNTVSPWYTDTPLAEPMLKNPERFRRIIERTPMNRVA
                     QAEEVASVVAFLAMDKASYITGHNLVADGGMLVNGL"
     gene            1642..3507
                     /locus_tag="VGQ51_12445"
     CDS             1642..3507
                     /locus_tag="VGQ51_12445"
                     /inference="COORDINATES: protein motif:HMM:NF039702.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6250 domain-containing protein"
                     /protein_id="HEV9037432.1"
                     /translation="MKKACLLSLLLLFLARAFPAVIYVSVDGNDKNPGTKDQPLATIQ
                     HAQDGVKPGDTVYIRGGIYHVPADGIARRERIYACITFLDKSGRPGRYINYWAYPGET
                     PVFDCSAVKPEGWRVAAFYVTGSWLHLKGFQVTGVQVTIKTHTQSECFENHGSHDIYE
                     GLSMHDGMAIGFYLLSGSDNLILNCDAYRNFDYFSENGRGGNTDGFGCHPDAGSSGNI
                     FRGCRAWFNSDDGYDCINAHEATVFDHCWAFYNGYTPDFKSEGDGNGFKGGGYARRPA
                     DQFPHPIPRNTIEYCVAVRNKANGFYSNHHPGGSNWFNNTAYRNNVDYNMLNRLDDPY
                     SDVAGYKHVLKNNIGFAGGREVENLDTSQCELSGNIFGDDLSDSDFLSLDETQLTAPR
                     QPDGSLPAITFMHLRPSSRHSLILSDDFGRTLDPHTWIAEIEPKPGGISSVYTRDGAL
                     ILNTRAGVTLWLNRQLTGDNRIEFDREVIVDTGHNDRLSDMNVFWMASDPKNPNLFTR
                     DGRLAGYDDLRLYYVGMGGNTNKTTRFRKYSNGERKLIAEYTDAPHLLRANKIYHIAI
                     LTQKGTTSFWVDGNCFFSYADPEPLTAGYFGFRSTWSRQAISHFRVYSAATPGQN"
     gene            complement(3498..4997)
                     /locus_tag="VGQ51_12450"
     CDS             complement(3498..4997)
                     /locus_tag="VGQ51_12450"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEV9037433.1"
                     /translation="MLFNSYTFIVFFAIILILHNLPFPWKVKKINLLIASYLFYAAWN
                     PPFILLLWLSTVVDFFVGRALYTQNNTHKKRLLLVISLIGNLGMLCFFKYGGFLLDNF
                     VALTHLIGVPFHPAKPNIILPAGISFYTFTTLCYTIDMYKKESKPVRSFLDFALFVTF
                     FPHLVAGPIVRPPQLVPQFESPRRASSKQLYEGLLLLTVGLFMKVVLADSMLSTTADT
                     IFSGSGNLPVLDAWMGVLAFSGQIFFDFAGYSTCAVGAAYCLGFVLPQNFRYPYAAIG
                     FSDFWRRWHITLSSWLKDYLYIPLGGNRKGTARTYFNLMVTMLLGGLWHGANWTFVAW
                     GGLHGFYLWVEKFFRDRKSRLSERLATAGSSLVETRPAPVQASLAPPVGVSGTGRGFI
                     FALLTFFLINVTWVFFRASSFGMAGHMLVSMFGGAQGAVPILTNLAITKVVVIVTAMI
                     FVHWFMRNRRMLDLVYEIPWWLTGVLWAVILLLLILTQSSSASFIYFQF"
BASE COUNT         1280 a         1485 c         1286 g         1125 t
ORIGIN      
        1 ggagccactg aatacctggg aacacatctt tgaaaaggat gggaaggcga atgactatct
       61 ctatttatac gatcagctaa gtaaggaatt catccgggga acgccgggtg gtccggcttc
      121 cgccggcacc aatgttttaa cgtttcatta aacaaccagg tgctacggcc ggccgttttt
      181 tgtcgtcaaa cgctaatatg aaacaacacc gccctctccg gtattgggct tggctattcc
      241 cggtcctgct gactgcggcc tgcaagaaag acccttataa aatggcagcg ccgcgcgcca
      301 tcaattatat cctatacacc gaaaaggatt tctccaacga ccacgacacg atccggttcg
      361 aaattctcat gagatcagga agtaaggttc tgttggattc tccgcttgct cccatgacga
      421 tcgcgcagat tccggactcc tttcaccgga tcatgttcca gaaattcgtc ccatccgcca
      481 atcagaatga cgatcttgtc gtagggtttc tttatcagat agataatgtc ggcatgtcct
      541 ggttcctcga ttcgagcaaa gcgagtggtc cgtcggtaaa gaccgtcacg tacagctttg
      601 agtaagggtt cgacatagac tggaccgccg tcgccggctc cctccggcgc ggcctgctcc
      661 cgcgcccgcc taaagcccat tcacaagcat cccgccatcc gccacgaggt tatgcccggt
      721 gatataagaa gccttgtcca tcgccagaaa ggccacgacg ctggcgactt cttcggcctg
      781 cgccacgcgg ttcatcggtg tccgttcgat gatacgccgg aatcgttcgg gattcttcaa
      841 catcggctcc gccagcggag tatcggtata ccatggggaa acagtattga cgcggatgcc
      901 atgttcggcc cattcgacgg cgagatggcg gctaagctgg atcaaggctg ccttggacat
      961 tccatagggc gcgcccgttt gtacatcgac acaacccgcg accgaggcga tattgacgac
     1021 actcgcggca tgaccatgca ccagcagcgg cagacacagg cggcataatt cgataatgct
     1081 gaaaaggttc agctcgagta ccgaccggta ttcttcctcg gcatactgaa ccagcctttt
     1141 ccggatgttg gtgccggcat tgttcaccag cacgtcaagt cctccccaac gctcggctac
     1201 ggcctcgatg atacgctgac ggcactcggg ccggctgaca tctccggcta ctcccgtcac
     1261 catccttctt tccgtttgcc acagtcttac gagacggttc acttctcttt cgtcccgcgc
     1321 cacgatcata acatcggctc ccagcccgag gaactcttcg gcaatggctt tcccgattcc
     1381 cttggtccct ccggtgataa gggcacgttt gccctgcagg tcccattttt tcatggttga
     1441 aaaaaattat ttttactaat atacgcactt tccccgccgc cccccaccaa ccttccgtct
     1501 gttcccacca atcttcccgc cgcccccacc aatcttcccg ccgcccccac caatcttccc
     1561 ctcggtcccc accaatcttc ccgccacccc caccaagacg gtgcaggatg gatatctcaa
     1621 acgaatgtct aacttttggg tatgaaaaaa gcttgcctgc tgtccttgct cctcctgttt
     1681 ctcgcccgcg cttttcccgc cgtcatctat gtctcagtgg acggaaatga taagaatccc
     1741 ggaacgaaag accaacccct tgccaccatc cagcacgcgc aggacggcgt gaaacccggt
     1801 gataccgtct atatccgcgg cggtatttat catgtacccg cggatggcat tgcccgccgc
     1861 gaacgtatct atgcctgcat caccttcctc gacaaaagcg ggcgaccggg cagatatata
     1921 aactattggg cctatcccgg ggagacgccg gtattcgact gtagcgccgt caagccggaa
     1981 ggctggcggg ttgcagcatt ttatgtcacc ggatcctggc tgcatctgaa aggtttccag
     2041 gtcacgggcg tgcaggtgac gataaaaaca catacccaat cggaatgttt cgagaatcac
     2101 ggcagccatg acatctatga gggactgagc atgcatgatg gtatggccat cgggttctat
     2161 ctattgtccg gatcggacaa tctgatcctg aattgtgacg cctataggaa tttcgattat
     2221 ttctcggaga atgggcgtgg cggcaatacc gacggattcg gctgtcatcc cgacgccggc
     2281 agcagcggca acattttcag gggatgccgc gcctggttca acagcgatga cggctacgat
     2341 tgtatcaatg cgcatgaggc cacggtattc gatcattgct gggcgttcta caacgggtat
     2401 acacctgatt ttaaaagtga aggggacggt aacgggttca agggcggtgg ttatgcccgg
     2461 cgaccggctg accagttccc tcatcccata ccgcggaata ccatcgaata ttgtgttgcg
     2521 gtacgtaata aggccaacgg tttttattcg aaccatcatc ccggcggcag caactggttc
     2581 aacaatacag cttaccgcaa caatgtcgac tataacatgc tcaaccgcct cgacgacccg
     2641 tattccgatg tggcgggtta caaacacgta ttgaagaata atatcgggtt tgccggggga
     2701 cgcgaagtgg agaatctcga tacgtcgcaa tgcgagctgt ccgggaatat tttcggtgac
     2761 gacctttcgg attcggattt cctcagcctc gatgaaactc agctcaccgc accccggcag
     2821 cccgacggaa gcctgcccgc gatcaccttt atgcatcttc ggccgtcgag ccgccattcc
     2881 ctcatcctca gcgatgattt cggacgtacc ctcgaccctc atacatggat cgcggagatc
     2941 gaacccaaac ccggcggcat ctccagcgta tatacgcgcg acggcgctct catccttaac
     3001 acacgcgcgg gcgtaaccct gtggctcaac cgccaactca ccggcgacaa ccgcatagaa
     3061 ttcgatcgcg aagtcatcgt cgacactggc cataacgatc gtctgtccga tatgaatgtc
     3121 ttctggatgg cctccgaccc taaaaatccc aacctcttca cgcgtgacgg ccgtctggcc
     3181 ggctatgacg acctgcggct ctattatgtc gggatgggtg gcaacaccaa caagaccacg
     3241 cgcttccgaa aatattccaa tggcgagcga aagctcattg ccgaatatac cgatgccccg
     3301 catttactcc gggccaataa aatataccat atcgcgattc tcactcaaaa gggcacgacg
     3361 agtttttggg tcgacgggaa ttgctttttc tcctatgccg atcccgagcc gctgacggca
     3421 ggatatttcg gtttccgcag cacctggtcc cgccaggcga tcagccattt cagggtctac
     3481 tccgccgcga cacccggtca gaattgaaaa taaataaagg aagcactact cgattgcgtc
     3541 aggatcagga gcagcaggat cacggcccat aatacgcccg tcagccacca gggtatttca
     3601 tagaccaggt caagcatacg cctgtttcgc ataaaccagt gcacgaaaat catcgccgtg
     3661 acgattacca cgaccttggt gatcgcgaga ttcgttaaaa taggtacagc accctgcgcc
     3721 cctccgaaca tagataccag catatgacct gccataccaa aactggaggc gcggaaaaat
     3781 acccaggtta cattgatcaa aaagaacgtc agcagcgcaa agataaatcc tctgccagtg
     3841 ccggacacgc ccaccggggg agccaggctg gcttgcaccg gagccggtct tgtttccacc
     3901 aatgaagacc ccgccgttgc caacctttcg gagagccggg acttcctgtc gcggaaaaat
     3961 ttctccaccc ataaatagaa accatgcaat cctccccagg ccacgaaagt ccagttcgca
     4021 ccatgccaaa gaccacccag cagcatcgtc accataaggt tgaaatacgt cctcgctgta
     4081 cccttcctgt tccccccgag tggaatatag agatagtcct tcagccagga agaaagcgtg
     4141 atatgccatc tcctccagaa atcggagaac ccgatggcgg catagggata acggaaattc
     4201 tgcggcaaca caaaacccag acagtatgca gctccgacgg cacaggtgga ataaccggca
     4261 aagtcgaaga agatctgacc cgaaaaagcc agtacaccca tccatgcgtc caataccggc
     4321 aaatttcccg atccgctaaa gatggtatcc gccgtagtgg aaagcatgct gtcggccagg
     4381 accactttca tgaacaatcc gacggtcagc agcagcagtc cttcgtataa ttgtttggaa
     4441 ctcgccctcc gcggagattc aaactgcgga accagttgag gcggtcggac gatcgggccg
     4501 gccaccagat gcggaaaaaa ggtcacgaag agagcgaaat caagaaagga cctgactggc
     4561 ttgctctcct ttttatacat atcgatcgta taacacagtg tcgtaaacgt ataaaaggat
     4621 attcccgccg gaagtatgat attgggtttg gctggatgga acgggacacc tatcagatgc
     4681 gtcagcgcaa caaaattatc cagcaggaac ccgccgtatt taaagaaaca gagcatgccc
     4741 agattgccga tgagactgat caccagcagc agcctctttt tatgcgtatt gttttgggta
     4801 tacagcgccc tgccgacgaa aaaatctact acggtagaga gccagagcag caggataaag
     4861 ggaggattcc aggcggcata gaaaagatag ctggcgatca gcagattgat ctttttcact
     4921 ttccatggga aagggagatt gtgcaggatc aggatgatcg cgaaaaaaac gatgaaagta
     4981 taagaattga ataacatgat tcgggtgttt gggatgggtt agtgaagata ttcggcgggc
     5041 agcgcatcga tcagggcctt tgtatagagc acggcatccg aaggtttcag atgggaccat
     5101 tcagggcacg taaaatggct catcgaagga tagtctttat agtagatgcc tttgcaaccg
     5161 gcggcctgaa gcaaat
//
LOCUS       DASYSV010000405         6999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_721335, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000405 DASYSV010000000
VERSION     DASYSV010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721335"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2525)
                     /locus_tag="VGQ51_12455"
     CDS             complement(<1..2525)
                     /locus_tag="VGQ51_12455"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9037434.1"
                     /translation="MRVTNRLFRAHVLGGGRSAAESLHTKLFFLLLLVVPTWLYAGEN
                     PRVKYLGIEQGLSNNAVTCMCRDGKGFMWFGTYDGLNRYDGYGFTVYRNIIGDSTSLI
                     CNNIASLVADSHDNIWVSSQKGISILDIVSGRFAVPSYLSCDGRGVVKVRDNVHMVKP
                     IGSRYILAGSHSKGLIVFEGGSRIGRQLQLEGSGGDYDVFSIENDPGSGRVWVFIEGK
                     GLYGLDLGAMKLSLVSGAIKEAFCLRVDNKGRLWVGTDEGLFTFDKVKNVYSDNYLPE
                     KSRVVDIDVNRQNDLCLGTDGTGVWRLPDGARTAIPLVSPGGSPLVNSNAVYAVLEDD
                     DGRKWIGTLRGGINVIEPRTSSFRTIVYDPPGRKNIVNNFILSFCEDADHSIWIGTDG
                     AGLRCWNRKTNQFREYVNDPADKGSINSNFITGILRDDQDELWVSTWFGGINRLDRRT
                     GKFEHFVCFNPVKGEEEKHIWTMIEDRLHRIWASATNDGALYQLDRKTGRFVLFDSRI
                     QSLQALIEDREGNLWGGNYTQLIRIDPVGRHHRIYPLGYTIRSIHEDRSGRLWVGTQE
                     GGLLLFDPATGEYKRYTTTDGLPSNTILRILEDSHGNLWMSTYNGLSERLCADGKFRN
                     FSPSDGLQSTQFSFNASIALSTGEFLFGGIKGFNLFFPDSVLQRAPMPPVYLTQVRIQ
                     NDPVQGDSKYVSELNGGAIRQLTVPFNKATLSLDFTALEYDGTDKINYSYCLANWDKG
                     WNDAKGSRTANYSSLREGTYFFLVRVSHADGIWSRQKDMLCVIVLPPWYRTWWAYLLA
                     GLLAGGLVVLYVRYTRSKLRLKYEIRLAQLETEKEKELIERKLS"
     gene            complement(2722..3600)
                     /locus_tag="VGQ51_12460"
     CDS             complement(2722..3600)
                     /locus_tag="VGQ51_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV9037435.1"
                     /translation="MNRGLSAGRGRVRAGLLAGALLLAGGAVLAQGRPLYTSPLGVEA
                     YTYRNSWPRGVVQTLDTIKMLGFTELEGGGGRMDPREFRKLCDERGIRIPSIGADYNK
                     LLRAADSVIDQAKILGAKYVMCAWIPHKDGVLTFDDARRAVDDFNKVGKVLSEHGLIF
                     CYHAHGYEFQPYEGGTLLDYIVTHTDPKYVSFEMDIFWISFGGGDPVGLMKKYGSRWK
                     LMHLKDLRKGAPKNLTGLTGPENDVPLGQGEEDIPAVLREAKRIGITHFFIEDESVDP
                     TVPVPQSIAYLKSLRD"
     gene            complement(3597..5267)
                     /locus_tag="VGQ51_12465"
     CDS             complement(3597..5267)
                     /locus_tag="VGQ51_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV9037436.1"
                     /translation="MNYDAIVIGSGASGGWAAKELCEKGLKVLVLERGRDVRHIKDYP
                     TASKGPWEFPHRGTVPLVERAAYQGSAFVREETLHWALPADEQPFIQEKPFRWVRGYH
                     TGGKSLLWARQTQRWSDYDFEGPGRDGFAVDWPIRYRDLEPWYTYVEKFAGIAGNRDG
                     LAELPDSVVMPGFEMSCVEQYFKESLAAHYSDRHLIQARCAHLTDPQPVHLAQGRVKC
                     QNQNMCVRGCVFGGYFSSNASTLPWAMKTGNMTLRPHSVVHSIIYDDKKGKATGVRVI
                     DAETKQTLEFFAPLIFVNASTLNSNAILLNSISGRFPNGLGNDNGLLGKFVSCHNYRG
                     KGSASFDGFRDRMVDGRNPSNSYMPRFRNLRKQDSGFLRGYAIGIGGGRGPATDTSGI
                     GDALKERLLHPPLGDWYISSWMMGESVPVEKNHVRLSKDLQDKYGIPQLIVSVEWGDN
                     DDKMVADYIQQMGEMFEKAGFKHIDVHDSKAPPGSDIHEMGGVRMGHDPKTSLLNKWN
                     QLHHCPNVFVTDGACMTSTSCQNPTLTYMALTARAAAHAVEELKKGNL"
     gene            complement(5272..5718)
                     /locus_tag="VGQ51_12470"
     CDS             complement(5272..5718)
                     /locus_tag="VGQ51_12470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEV9037437.1"
                     /translation="MKRRKAIKDLLVASGGALALPWWMEGCRTGEKGVGVLPVVVDTI
                     VPGAVELGVDRYVDRMIADCYEQPVQENVRKQLAALEGKGFVSASKQQREAMLLELTE
                     KDEKDFFAVIKSETIRGYTTSQKVMEEYFHYKVAPGVYSGCVPVHA"
     gene            5778..6644
                     /locus_tag="VGQ51_12475"
     CDS             5778..6644
                     /locus_tag="VGQ51_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037438.1"
                     /translation="MKYLLFFALPFFFLAADPGYRLIRMTSPHNSFPDTARDNGHLDG
                     DHIFQPKAGHYDDSSVILVVPKNFHPQKNIDLVFWYHGWHNNIDTALQFYGLARQFAA
                     SKVNAILVLPEAGKNVADSYGGKMNQKGMFRLLVQDVMDQLKKQHLATSNAEPGHITV
                     GGHSGAFLVISNLLDYGDQPVDEVFLFDALYSHVPSFMDWIGKDRAQHHFVHWFTNKG
                     GGTDEVSDTMMLRLKEKGWPFRLVEENAVNPAILKNNNILFVHSPREHNVIINNPDDF
                     QLLLENSMHLTR"
     gene            6807..>6999
                     /locus_tag="VGQ51_12480"
     CDS             6807..>6999
                     /locus_tag="VGQ51_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037439.1"
                     /translation="MFLLIFGILLLIAGFVTARRTDSFKRYGNPLKLVGFLIALLGFS
                     SASLVQIGAGEVGVQILFGK"
BASE COUNT         1611 a         2316 c         1455 g         1617 t
ORIGIN      
        1 gacaacttcc gctcgatcag ctctttttcc ttttcggtct ccagctgcgc cagtctgatc
       61 tcgtatttca aacgcagttt gctgcgcgta tatcgcacat aaagaacgac caggcccccg
      121 gctaacaacc cggccagaag ataggcccac caggtacgat accacggcgg caatacgatt
      181 acgcaaagca tatccttttg tctgctccag atgccgtcgg catggcttac ccgcaccagg
      241 aagaaataag tgccttcccg cagactcgaa taattcgccg tccggcttcc tttcgcatcg
      301 ttccaaccct tatcccagtt cgcgagacaa taactatagt tgattttatc cgtcccatcg
      361 tattccagcg ccgtgaaatc cagcgacaag gtggccttgt tgaacgggac cgtcaattgg
      421 cggatggctc cgccattcag ttcggataca tatttggaat cgccctgcac aggatcattc
      481 tgtatccgca cctgcgtgag atagacgggt ggcatcggcg cacgctgtaa gacactgtcg
      541 ggaaaaaaaa gattgaaccc cttgatacct ccgaacagga actcgcccgt agacaatgcg
      601 atcgacgcat tgaagctgaa ttgcgtgctt tgcaatccgt cggaagggga aaaattcctg
      661 aacttcccat cggcgcaaag ccgttcggat aatccgttat acgtgctcat ccataagttt
      721 ccatggctgt cttccaatat gcggagaatg gtgttgcttg gaagaccatc ggtggtcgtg
      781 tagcgtttgt actcccccgt tgcaggatcg aataacagca gacccccttc ctgggtgccc
      841 acccacaatc tccccgatct gtcttcatgt atggaacgta tcgtatagcc cagcggatag
      901 atacggtgat gccggcccac ggggtcgatc cggatgagtt gggtgtaatt gcctccccat
      961 aaatttccct ctcggtcttc gatcagggcc tggagactct gtatccggga gtcgaacagg
     1021 acaaaccgac ccgtcttccg gtcgagctgg taaagggcgc cgtcattggt ggcgcttgcc
     1081 catatcctgt gcagacggtc ctcgatcatc gtccagatat gtttttcctc ctcccctttc
     1141 accgggttga aacaaacgaa atgctcgaat ttacccgtcc gccgatcaag ccggttgata
     1201 cctccgaacc aggtcgacac ccatagctcg tcctggtcat cccgcaggat ccccgttata
     1261 aaattactgt tgatcgatcc tttgtccgcg ggatcattga catactcccg gaattgattg
     1321 gtctttcggt tccagcacct caatccggcc ccgtccgtgc cgatccagat gctatggtcg
     1381 gcatcttcac aaaaagaaag gatgaaattg ttgacgatat ttttccggcc gggaggatca
     1441 tagacaatgg tccggaatga gctcgtcctc ggctcgatga cgttgatccc accgcgaagc
     1501 gtccctatcc atttccgccc gtcgtcatct tccagcaccg catagacggc attgctgttc
     1561 accagggggc ttccgccggg agacaccaat gggatagccg tcctcgctcc atccgggagc
     1621 cgccatacac ccgtgccatc ggtaccgaga cagagatcgt tttgccggtt gacatctata
     1681 tccacaaccc ggctcttctc cggcagataa ttatccgaat aaacattctt caccttgtca
     1741 aacgtgaaaa gtccttcatc cgtgccaacc cacaacctgc ccttattatc gaccctgaga
     1801 caaaaagcct ctttgatcgc cccactcacc agtgacaatt tcatagcccc caggtccaga
     1861 ccatacaatc cctttccctc gataaacacc catacccgtc cggaaccggg atcgttctcg
     1921 atggaaaaaa catcataatc cccgcctgat ccctccaact gcagctgtcg tccgatcctc
     1981 gatcctccct cgaatacgat cagcccctta ctgtgtgagc ccgcgaggat ataccgtgaa
     2041 cctatcggct tgaccatatg aacattatcc cgcactttca cgacacctct gccgtcgcag
     2101 gacagataag aagggaccgc gaaccttccc gacacaatgt ccaggatact gatgcctttc
     2161 tgactgctga cccatatatt atcatggctg tccgcgacga gacttgcgat attattacag
     2221 atcaggctcg tggagtcccc gatgatattc ctgtagaccg taaatccata tccgtcatac
     2281 ctgttgagtc cgtcatacgt tccaaaccac ataaagccct tcccgtccct gcacatacag
     2341 gtcacggcgt tgttcgatag cccctgctcg atacccaaat acttaacccg cggattctca
     2401 cccgcgtaca gccacgtcgg caccactaat aaaagaagga agaacagctt cgtatgcaaa
     2461 ctctccgcgg cagatcgccc cccgcccaac acatgagccc taaacagtcg attggtcact
     2521 ctcatatagc agtaatcgta aatataaaat tacgcgatta ctgtcatttt aacattaaat
     2581 attcactaca acgttgtcgt aatcgaccaa gtcccccacg ctctctcagt ccttccagcg
     2641 ttcccagtcc ctcgggctct cagcccccac gctctctcag tccttccagc gttcccagtg
     2701 ctctcagccc ctcacgctct ctcagtccct caggctcttc agatacgcga tactctgcgg
     2761 tacgggcacg gtcgggtcga cgctttcatc ctcgataaaa aaatgcgtga tcccgatcct
     2821 cttcgcctcg cgcaacaccg ccgggatgtc ttcctccccc tgtcccaacg gcacatcatt
     2881 ctccggcccc gtcaatcccg tcagattctt cggcgcaccc ttccgcagat ccttcaaatg
     2941 catcagcttc caccgcgatc cgtatttttt catcagtccc acaggatccc cgccgccaaa
     3001 cgatatccag aatatatcca tctcgaaaga cacatatttc ggatccgtat gagtcacgat
     3061 ataatccagc agcgtcccgc cttcatacgg ctgaaattca tacccatgcg catgatagca
     3121 aaagatcaat ccatgctcgc tcaatacctt ccccacctta ttgaagtcat ccaccgcccg
     3181 ccgcgcatcg tcgaaggtca gcaccccgtc cttatgcggt atccacgcac acatcacata
     3241 tttggcgccc aggatcttcg cctgatcgat caccgaatcc gccgcccgaa gcagcttatt
     3301 atagtcggcg cctatcgagg gaatcctgat cccgcgctca tcacacagtt tcctgaattc
     3361 ccgcggatcc atccggcctc cgccaccctc cagctcggta aaacccagca tcttgatcgt
     3421 gtccaatgtc tgaactacac ccctcggcca gctgttccgg taggtatagg cctccacacc
     3481 cagcggcgac gtatataacg gccgcccctg cgccaatacc gcaccgcccg ccaacaacaa
     3541 tgctcccgct aacaatccgg ccctcacgcg ccctcgcccc gctgacaatc ctctattcat
     3601 aagtttcctt tttttaattc ctccaccgca tgcgccgccg cccgcgccgt caacgccata
     3661 taggtcagcg tcggattctg acagctggtc gacgtcatac acgccccgtc cgtcacgaac
     3721 acattcggac agtgatgcag ctggttccat ttattcaata aagatgtctt cggatcatgc
     3781 cccatcctca cgccacccat ctcatggatg tcgctgcccg gcggcgcctt cgaatcatgt
     3841 acatcgatat gcttaaagcc ggccttctcg aacatttctc ccatctgctg aatataatct
     3901 gcgaccattt tatcgtcatt gtcaccccac tccaccgaaa cgatcaactg aggaatgcca
     3961 tatttatctt gtagatcctt actcaaccgc acatgattct tctccaccgg cacgctttcc
     4021 cccatcatcc aactgctgat ataccaatcc cccagcggag gatgcagcaa acgctctttc
     4081 aatgcatccc cgattccact cgtgtccgtc gcagggcccc gtcccccgcc gatgccgatc
     4141 gcatatcccc gcaaaaatcc ggaatcctgt ttgcgcagat tcctgaaccg cggcatatag
     4201 ctattgctgg gattacggcc gtccaccatc ctgtctctga acccgtcgaa acttgccgat
     4261 cctttaccgc gataattatg acaggaaacg aatttgccga gcaggccgtt gtcatttcca
     4321 agaccattcg ggaatcgtcc cgaaatcgaa ttcagcagga tagcattcga attcagcgtc
     4381 gatgcattga cgaagatcag tggcgcaaag aactcgagcg tctgtttcgt ctctgcgtcg
     4441 atcaccctaa ccccggtagc cttccctttt ttatcatcgt agataataga atgcaccacc
     4501 gaatgcggcc tgagcgtcat attgcctgtc ttcatggccc agggcagcgt cgaggcattg
     4561 ctgctgaaat atccgccgaa tacacatccc cgaacacaca tgttctgatt ctgacacttg
     4621 acccttccct gcgcaaggtg cacgggctgc gggtccgtca aatgcgcgca tcgcgcctgt
     4681 atcaaatgcc ggtcggagta atgcgctgca aggctctctt taaaatactg ctccacacag
     4741 ctcatctcaa aacccggcat gaccacgctg tccggtagct ccgccagtcc atcccggttc
     4801 cccgcgatac ctgcaaattt ctccacataa gtataccacg gctccaggtc cctgtaccgt
     4861 atcggccagt ctacggcaaa cccatcccgg cccggcccct cgaaatcgta atcgctccac
     4921 cgctgcgtct gtctggccca taacaatgat ttgccgcccg tatgatatcc ccggacccaa
     4981 cgaaaaggtt tctcctggat gaagggctgc tcatccgccg gcaacgccca atgcaaggtc
     5041 tcctcgcgga cgaaggcaga accctggtaa gcggcacgct ccaccaatgg cacggtcccg
     5101 cgatgcggga attcccaagg ccctttcgac gccgtcggat agtccttaat atgccggaca
     5161 tcgcgacccc tctccaatac caacacctta agcccctttt cgcaaagctc tttcgccgcc
     5221 catcctccgc tggcgcccga tcctatgaca atggcatcgt aattcatgca attatgcgtg
     5281 tacgggtaca cacccggagt agacgccggg tgcgacttta tagtgaaaat attcttccat
     5341 tactttctgc gaggtcgtat acccgcggat ggtctcgctc ttgatgaccg caaagaaatc
     5401 tttctcatcc ttctctgtca actccaacaa catcgcctcc cgctgctgtt tcgacgcgga
     5461 aacaaacccc ttcccctcca atgccgccag ctgtttccgc acattctcct gcaccggctg
     5521 ctcataacaa tccgcgatca tccggtccac ataccggtcg acacccaatt ccacggcacc
     5581 gggcacgata gtatccacta ccaccggcag cacgcccaca cctttctcac ccgtccggca
     5641 accctccatc caccacggca acgcgagagc cccgccagac gcaaccaata ggtcctttat
     5701 agccttgcga cgtttcatac gcattcaatc tacacaaaat ttaaatccct tcggcaattt
     5761 tccctatttt tcccggcatg aaatacctat tattcttcgc ccttcctttt ttcttcctgg
     5821 ccgccgatcc cggctaccgt ctcatccgca tgacctctcc gcacaacagc tttcctgaca
     5881 ccgccaggga caacggacac ctcgacggcg accatatctt ccagccgaaa gccgggcact
     5941 acgacgattc ctccgtcatc ctcgtcgttc ccaaaaattt ccatccacaa aaaaacatag
     6001 acctcgtctt ctggtaccac ggctggcaca acaacatcga taccgctttg cagttttacg
     6061 gcctcgcccg ccaattcgca gcatcgaagg tcaatgccat tcttgtcctc cccgaggccg
     6121 gaaagaacgt cgccgacagc tacggcggca aaatgaacca gaaaggcatg ttccgtctcc
     6181 tcgtacaaga cgtcatggac caactaaaaa aacagcatct cgccacatcg aacgccgaac
     6241 ccggccacat tacggtcggc ggccacagcg gcgccttcct cgtcatctcc aatctcctcg
     6301 attacggcga ccaaccggtc gatgaagtct tcctcttcga cgcactctac agccatgtac
     6361 cctcattcat ggactggata ggtaaagaca gggcgcaaca tcacttcgta cactggttca
     6421 ccaacaaggg cggcggtacc gatgaggtca gcgacacgat gatgcttcgt ctaaaagaaa
     6481 aaggctggcc tttccggtta gtcgaagaaa acgcggtaaa ccccgctatc ctcaaaaaca
     6541 acaacatcct cttcgtccac agcccgagag aacacaacgt catcatcaat aacccggacg
     6601 attttcagct attgctcgaa aattccatgc accttaccag ataacagcct ccgccgccgc
     6661 gtgaaacgcg gttgcatttc cgttatgagc gattgtgtca taatcagtta ttgggcaacc
     6721 gcactccggc gacccccgcc gctcatacac aaataacgtg tatgcacccc ctttccgcca
     6781 tagctttaca tcctaaactc aaccatatgt tccttctgat cttcggtatc ctccttttaa
     6841 tcgccgggtt cgtcacagcc cgcaggaccg attcctttaa acgttacggt aaccctctca
     6901 agctggtcgg ttttctcatc gccctgctgg gtttttcttc cgccagcctc gtacagatcg
     6961 gcgccggtga ggtcggcgtc cagatcctgt tcggtaaag
//
LOCUS       DASYSV010000406         5530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1744385, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000406 DASYSV010000000
VERSION     DASYSV010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744385"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VGQ51_12485"
     CDS             <1..1153
                     /locus_tag="VGQ51_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9037440.1"
                     /translation="LILMDVQMPNLNGFETAALIYERERLRHIPIIFITANNYGEENL
                     FKGYRAGAVDYIYKPVNPELLRVKVGVFVDLYRKNQRLLAQEQKLVAINRSLELEINE
                     RIASEEKVTDLNKQLLENITRLETANKDLDLFAFMASHDLQAPLRKIRMFSDRLLANS
                     ENSFGKEGRLYLTRIQEVSKRMQDLINDILRFSKISVENHNFEDVDLNEVVGEVLSEM
                     EGNIKEKNARILIEDRLPVLPASTVLMGPLFSNLISNSIKYCKKNSRPEVHIRYEEAP
                     VASGANGRESETRYGRIYIEDNGIGFDQKYADQIFDMFRRLHSSAEYEGTGIGLALCK
                     KIVEMHNGYISALGKPGEGAVFIIALPLVPTQKELVEKNDEIRGQSSNN"
     gene            1162..2610
                     /locus_tag="VGQ51_12490"
     CDS             1162..2610
                     /locus_tag="VGQ51_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9037441.1"
                     /translation="MLHSNAPTRILIVEDDEDDFLIIDACIRDIPDKEFRIDWCYDYD
                     EALQRLGQCRYDIYFVDYLLGAKNGLELLKEAVAMGCEEPLVLLTGIGNRDVDVQAMT
                     IGAVDFLVKSEITTEKLERCIRYALERSAYIKALRVNERKFRNIFERSKDAVFLTTDD
                     LVFRDVNAATCDLFKFPKDELLQMSLYQLFARKEAAVNLKDRLSVTGEVEDLEVELHT
                     RNKERRNCILSISWQVYSTGETYIQGIIHDITNLKRIERATFQIEKLRSTATLLRTLA
                     HEVRNPLTNINLSVEQLKPEMDSEDANIYLDIIARNCGRIDGLISELLDLSRPAEISL
                     QKTRLQEIIDSTLAAASDRIALKNIRLELAYPERPAFVMADKDKLKIALLNILINAVE
                     AVPPKSGVITIAIREETPQHYKVSINDNGGGIPEENISRIFEPYFTSKTNGFGLGLAA
                     TWNILQSHRAGIDVSSQPGEGTSFMLTFEQAQ"
     gene            complement(2604..3272)
                     /locus_tag="VGQ51_12495"
     CDS             complement(2604..3272)
                     /locus_tag="VGQ51_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEV9037442.1"
                     /translation="MLNIYSIDYLDQVAPETFRSLLSRLPDVFRIKVQRYRRWQDAYG
                     SLFGKLLLRLALQRAGLSGELGTLRFNDYGKPFLENGPEFNLSHSGNRVVCVLSGQGR
                     VGIDLEAVSDLAIDDFQTQFTPAEWSRITGSATPLYTFYRYWTAKESLIKADGRGLQI
                     PLDQLVVGDAGETEGIALNENRWYIRELDAFPGYACHIACETTPFQVNIETLTWKDVE
                     GWSY"
     gene            complement(3294..3749)
                     /locus_tag="VGQ51_12500"
     CDS             complement(3294..3749)
                     /locus_tag="VGQ51_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV9037443.1"
                     /translation="MQSTIEHLEKIIRVYLRRMETLSDEAFEFRPRPDKWSRKEILGH
                     LVDSAQNNIRRFIVAQYEDTPNIFYAQDEWVKCSGYREYPVKDLIALWSSLNQHLVIV
                     LKQMGPDAAERLCSMGGAPQTLKWVAADYCNHLLHHLHQILDIEPLAYP"
     gene            complement(3755..4375)
                     /locus_tag="VGQ51_12505"
     CDS             complement(3755..4375)
                     /locus_tag="VGQ51_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV9037444.1"
                     /translation="MKRSLLLASLFTAGLIISAFRLAPGTLTPEERKYAVDYFNKTRE
                     RLLGDVRGLSEAQLNWKADSSRWSVYQCVEHIALSESFILHFAQDAMKPPATPDKRSE
                     VKYNPDQIIAMLTDRSHKAQAPEPIKPVKQFTDTKAALHAFLARRDSTIDYIKTTQDA
                     LKDHFIPGTPIGTLDAYGMLIFLAAHSERHTLQVEEVMANPNFPKH"
     gene            4456..5442
                     /locus_tag="VGQ51_12510"
     CDS             4456..5442
                     /locus_tag="VGQ51_12510"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEV9037445.1"
                     /translation="MRQIAFREALREAMNEEMRRDDRVFLMGEEVAEYNGAYKVSQGM
                     LDEFGPKRVIDTPISELGFTAVAVGAAQNGLRPVVEFMTWNFAVLALDQILNTASKML
                     AMSGGQVGCPIVFRGPNGSAGQLGAQHSTAFESYYANIPGIKVVSPSNGYDAKGLLKQ
                     SIRYEEDPVIFMESEVMYGDKSEVPEEEYYIELGKCDVKKAGKDVTIVSYNKMMKVAL
                     GAAAELEKENISAEVIDLRSIRPLDWMTILESVKKTNRLVIVEEQWPFASISSEISYR
                     IQKEGFDYLDAPIRRITSADAPLHYAPNLVAAALPDVKRTVKLVKEVMYLVK"
BASE COUNT         1341 a         1450 c         1521 g         1218 t
ORIGIN      
        1 cctgatcctg atggacgtgc agatgcccaa tctgaatggt ttcgaaacgg ctgcgctcat
       61 ctatgaaaga gagcggctac ggcatatccc gatcattttc atcactgcga ataattacgg
      121 cgaggaaaat cttttcaagg gctatcgcgc aggagcagtg gattatatct acaagccggt
      181 gaaccccgaa ttgctgaggg tgaaggtcgg cgttttcgtc gatctctatc gcaagaacca
      241 gcgtctgctc gcccaggaac agaagctggt agccatcaac cgcagcctgg aactcgagat
      301 caacgaacgg atcgcctcgg aagaaaaggt caccgatctg aacaagcaat tgctggagaa
      361 tatcacccgg ctcgagaccg ccaataagga cctggacctg ttcgccttca tggcatcgca
      421 tgaccttcag gcgccgctgc ggaagatccg catgttcagc gaccggctgc tggccaattc
      481 cgagaattcg ttcggcaaag agggccggtt ataccttacc cgcatccagg aggtttccaa
      541 acggatgcag gacctcatca acgacatcct ccggttctcc aagatctccg tcgaaaacca
      601 taatttcgag gacgtggatc tgaatgaggt cgtcggggag gtgttgtccg aaatggaagg
      661 gaatataaag gaaaagaatg cccggatcct gatcgaggac cgtcttcccg tgctgcctgc
      721 cagcaccgtc ctgatgggac cgctgttctc caacctgatc agcaattcca tcaagtactg
      781 taaaaagaat tcccggcccg aggtccatat ccggtatgaa gaagcgcctg tggcctccgg
      841 cgcaaatggc cgcgaatcgg agacccggta cgggcgcatc tacatcgagg acaatggcat
      901 cgggtttgat caaaaatatg cggatcagat cttcgacatg ttccgccggc tgcattccag
      961 cgccgaatat gaggggactg gtatcggcct cgccctttgc aaaaagatcg tcgaaatgca
     1021 taatggctat atctccgccc ttggtaaacc gggagagggg gccgtattta tcattgccct
     1081 gcccctcgtc ccaacgcaaa aagaattagt ggaaaaaaat gacgaaattc gcggacaatc
     1141 atcaaataac taattctcag aatgctgcat tcaaatgcgc ctacccggat actgattgta
     1201 gaagacgatg aagacgactt cctcatcatc gatgcatgta tcagagatat tccggataag
     1261 gaattcagga tcgactggtg ctatgactat gacgaagctt tacaacggct cgggcaatgc
     1321 cggtacgata tttatttcgt ggattatctg ctgggagcga agaacggttt ggaattgttg
     1381 aaggaggccg tcgccatggg ctgcgaggag ccgctcgtcc tgctcaccgg catcggcaac
     1441 cgggacgtgg acgttcaagc catgaccatc ggggccgtgg attttctcgt gaaatccgag
     1501 atcactacgg aaaaactgga gcggtgcatc cgttatgccc tcgaacggag cgcctacatc
     1561 aaggcattgc gcgtgaatga acgcaaattc aggaatatct tcgagcggtc taaagacgcc
     1621 gtattcctta ctaccgacga tctggtcttc cgggacgtca acgcggcgac ctgcgatctt
     1681 ttcaaattcc ccaaggacga gctcctgcag atgagcctgt accagctgtt cgcccgtaaa
     1741 gaggcggcgg tcaatttgaa ggacaggctg tccgtgaccg gggaggtcga agacctcgag
     1801 gtcgagctgc acacccggaa taaagagagg agaaattgca tcctttccat ctcgtggcag
     1861 gtttattcca cgggagagac ctatatccag ggcatcattc acgatatcac caatctgaaa
     1921 aggatcgaga gggccacttt ccagatcgag aagctgcggt cgacggccac cctgcttcgc
     1981 acgctggccc acgaggtaag gaaccccctc accaatatca atctttcggt cgagcagctg
     2041 aagcctgaaa tggacagtga agacgccaat atctacctcg acatcatcgc ccgcaattgc
     2101 ggacggatag acggactcat ctccgagctg ctggaccttt cccggcccgc cgagatcagt
     2161 cttcagaaaa caagacttca ggagataatc gactccacgc tggcggcggc atcggaccgc
     2221 atcgcgctca agaatatccg tctcgaactg gcctatcccg aacggcccgc tttcgtcatg
     2281 gcggacaaag acaaactgaa gatcgccctt ctgaatatcc ttatcaatgc cgtcgaggca
     2341 gtgcccccaa aatcgggtgt gatcaccatc gccatccgcg aagaaacgcc tcagcattat
     2401 aaggtgtcga tcaacgacaa cgggggaggt atccccgaag agaatatatc ccgcatcttc
     2461 gagccgtatt tcacctccaa gaccaacggg ttcggtctgg gactcgccgc tacctggaat
     2521 atcctccagt cgcatcgcgc gggcatcgac gttagctcgc agcctggtga agggacaagc
     2581 ttcatgctga ccttcgagca ggctcaataa gaccagcctt ccacatcttt ccaggtaagg
     2641 gtttcgatat ttacctgaaa gggagttgtt tcacaggcaa tatgacaagc gtagccgggg
     2701 aaagcgtcca gctcgcggat ataccacctg ttttcgttta acgcgatgcc ttccgtctcc
     2761 cctgcgtcgc caactaccaa ctgatccagc ggtatctgta aaccgcgtcc atccgccttg
     2821 atcaggcttt cttttgccgt ccaataccgg taaaaggtgt agagcggcgt tgcagacccg
     2881 gtgatcctgc tccattcggc cggagtaaat tgcgtttgaa aatcatcgat ggccagatcg
     2941 ctcaccgctt ccagatcgat gccgacgcga ccctgaccgc ttaggacgca gacgacacgg
     3001 ttgccggagt gggacaggtt gaattcagga ccgttctcca gaaagggttt gccataatca
     3061 ttgaaccgca gcgtcccaag ctcacccgac agaccggctc gttgcagggc cagtctcaac
     3121 agcagcttgc cgaaaaggct cccgtaagca tcctgccatc tcctgtagcg ctgaaccttt
     3181 atgcggaaga cgtccggcag ccgcgacagc aacgatcgaa aggtctcggg agccacctgg
     3241 tcgaggtaat cgatggaata aatattcagc atccttatcc ttgaatttac gggctatggg
     3301 tacgccaaag gttcgatatc gagaatctgg tgaaggtgat gcagcagatg attgcaataa
     3361 tccgccgcca cccatttcag cgtttgcggg gcgccgccca tcgagcacaa ccgctccgcc
     3421 gcatcgggcc ccatctgttt cagaacgatc acgagatgct ggttaaggct gctccataac
     3481 gcgatcaggt ccttcaccgg gtattcccgg taaccggaac atttcaccca ttcgtcctgc
     3541 gcataaaaga tattcggcgt atcctcatac tgggcaacaa tgaatctccg gatattgttc
     3601 tgcgcagaat ctacaagatg accaaggatc tctttgcggc tccatttgtc cggccgcggc
     3661 cggaattcga aggcctcgtc cgacaaggtt tccatccgcc ggagatacac acggatgatc
     3721 ttttcgaggt gttcgatggt agattgcatg ccggctagtg tttcgggaaa ttagggttgg
     3781 ccatcacctc ttcgacctga agggtatgcc tttcgctgtg ggccgcgagg aagatcagca
     3841 tcccatatgc atcgagcgtg ccgatcggtg ttcccgggat gaaatgatcc ttcagggcgt
     3901 cctgcgtggt ctttatatag tcgatggtag agtcgcggcg cgccagaaaa gcatgcaacg
     3961 cggctttggt atcggtaaat tgtttgaccg gcttgatggg ttccggagcc tgggccttat
     4021 gggaacggtc cgtcagcatc gcgatgatct gatcgggatt atatttcacc tcgctgcgtt
     4081 tgtccggtgt ggcggggggc ttcatcgcat cctgcgcgaa atgcaggatg aacgactcgg
     4141 agagtgcgat atgctctaca cattgataga cgctccagcg cgaagaatcc gccttccagt
     4201 tgagctgggc ctcgcttaga cccctgacgt ccccgagtag acgttccctc gtcttgttga
     4261 aatagtcaac ggcgtatttg cgctcttcgg gtgtcagggt gcccggggca agacggaaag
     4321 cgctgatgat caggccggcc gtgaagaggg atgccagcag aagcgatctt ttcatgtttt
     4381 ggtgttttgc catgaagata ataaatttgc ggctcgcccg ctaaggggtg ggataaacaa
     4441 aatattactt tacgaatgag acaaattgct ttccgggagg ctctgcgcga ggctatgaac
     4501 gaagagatgc ggcgcgatga tcgggttttc ttaatgggcg aagaggtggc tgaatacaac
     4561 ggcgcttaca aggtcagcca ggggatgctg gatgaattcg gtcccaagcg ggtgatcgat
     4621 acccctatct ccgaattggg ttttacggct gttgccgtag gggcggcgca gaacggcctt
     4681 cgtcccgtcg tcgagttcat gacctggaat ttcgccgtgc tggcgttgga tcagatcctc
     4741 aataccgcct cgaaaatgct ggccatgagc ggcggacagg tcggctgtcc catcgttttt
     4801 cgcgggccga acggctcggc cggtcagctg ggcgcacagc actctacggc attcgaaagc
     4861 tattatgcga atattcccgg tatcaaggtg gtcagtccca gcaacggata cgatgcaaaa
     4921 ggtctgttga aacagtccat ccggtatgaa gaggatccgg tgatcttcat ggagagcgaa
     4981 gtgatgtacg gtgataaatc agaggtgccg gaggaagagt attatatcga gctgggtaag
     5041 tgtgacgtta aaaaggccgg caaagatgtg accattgttt cctacaataa gatgatgaag
     5101 gtggcgcttg gcgcggcggc agaactggag aaagagaaca tcagcgccga ggtcatcgat
     5161 ctgcgcagca tacgtccgct ggactggatg accatcctgg aaagcgtcaa aaagacaaac
     5221 cgcctggtca tcgtggaaga acaatggcct tttgcgtcta tctcctcgga gatcagctac
     5281 cggatccaga aagaaggttt cgattacctg gatgcgccga tcaggaggat cacgtctgcc
     5341 gatgcgccgc tgcactatgc gcccaatctc gtcgctgcgg ctcttccgga tgtgaagcga
     5401 acggtaaaac tggtaaaaga agtgatgtat ctcgtaaaat agaaggaacg cttcgaaata
     5461 aaaagaaacc cgccataagg cgggtttttg ttttggtcat ttatgagcgc tatgagttat
     5521 ttgaaggcag
//
LOCUS       DASYSV010000407         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2385542, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000407 DASYSV010000000
VERSION     DASYSV010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385542"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VGQ51_12515"
     CDS             <1..648
                     /locus_tag="VGQ51_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEV9037446.1"
                     /translation="WIINPYDEWYALVRAIELKEKDPSTALHLVTVGAADTDPIIRKA
                     LAIGGDEAIRVNAESHDPYYIAFQIAEVARQGAYDLILTGKETIDYNGSAIGGMVAEM
                     LDLPYISLATKFDLNGATATVVREIEGGEETDEAALPLVVSCQKGVAEQRIPNMKGIM
                     SARTKPLKVVEPANAGPLTGIVSFELPPAKAGVKLVPADNPAELIRLLHEEARLF"
     gene            669..1634
                     /locus_tag="VGQ51_12520"
     CDS             669..1634
                     /locus_tag="VGQ51_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEV9037447.1"
                     /translation="MPVFIFIDHSDGHVKKASLEAMSYGAALAGKLGTSAEGILLGTL
                     PEDPSRLGRYGITKVHQAAQEGLNNLDTQVYAKIIADAVSAAGDSVVVFSNNLSGKAV
                     APRVAVRLKAAIVTGAVALPDISSGFVVKKNVFSGKAFANVSLLTPMKVIALNPNAYA
                     IKEGEGTAAIVSLTAGAPAPKVKVISATAASGHVPLTEAEIVVSGGRGLKGPENWGMI
                     EELAKVLGAATACSRPVADAHWRPHNEHVGQTGIAIAPNLYIAIGISGAIQHLAGVNR
                     SKVIVVINKDPEAPFFKAADYGIVGDAFEVVPKMIEAVKKLKGIN"
     gene            1722..2324
                     /locus_tag="VGQ51_12525"
     CDS             1722..2324
                     /locus_tag="VGQ51_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEV9037448.1"
                     /translation="MKKIELEIVALSHSITQTHSYAVVLGEVNGLRRLPIVIGGFEAQ
                     AIAVALEKMHPSRPLTHDLMKNFMSAFTIDLQEIIICDLQEGIFYSKLVCVSEHDTVE
                     IDSRTSDALALAVRFGCPIFTYEHILDSAGILMEDSSSKKKKTSSTATVSTTESSARE
                     DLKALSMDDLTSLLNEVLEQEDYIRAIAIRDEINSRRKTR"
     gene            2338..3153
                     /gene="ispE"
                     /locus_tag="VGQ51_12530"
     CDS             2338..3153
                     /gene="ispE"
                     /locus_tag="VGQ51_12530"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEV9037449.1"
                     /translation="MLFFPNCKINLGLKILRRRDDGFHDLETAFYPLPLKDVLEIVRT
                     TDETRFSSYGNPIPGDPGSNLCLKAWDLLRNDFPTLPPIHIHLYKNIPIGAGLGGGSS
                     DGASTLIVLNRQFQLGLTQQQLLDYAARLGSDCPFFILNKPCLGTGRGHLLQPLSLDL
                     SGYSFALIDPGIHISTAQAFSLCTPCENNTPLATILSHPVSDWREALINDFEEPIFRL
                     HPHLREIKDSLYDSGALYASLTGSGSSFFGIFTKDRAPLSNPFPDRYNYRILR"
BASE COUNT          766 a          977 c          801 g          691 t
ORIGIN      
        1 tggatcatca atccctatga tgaatggtat gctctcgtac gcgccatcga gctgaaagaa
       61 aaagatcctt cgaccgcgct ccatctcgta accgtcggag ccgccgacac cgaccctatc
      121 atccgcaagg ccctggccat cggcggcgat gaggccataa gggtcaatgc ggaaagccac
      181 gatccgtatt atatcgcatt ccagatcgcg gaagttgcac ggcaaggggc gtatgatctg
      241 atccttacag gaaaagagac catcgattac aacggatcgg ccatcggagg catggtagcc
      301 gaaatgctcg atctgcccta tatctcgctg gctacgaaat tcgatctgaa cggcgccacg
      361 gccacggttg tacgcgagat cgagggaggc gaggagaccg atgaagccgc tcttccgctc
      421 gtcgtcagct gtcagaaagg agtagccgaa caacgcattc ccaatatgaa gggcatcatg
      481 agcgcccgta ccaaaccgct gaaggtagtc gagcccgcca atgccgggcc gctgaccggt
      541 atcgtttctt tcgagctccc tcccgcaaaa gcaggcgtga agctcgtccc cgcggacaac
      601 cccgctgagc tgatacggct cctccatgaa gaagcaagat tattttaaac tcatctcaac
      661 atctcattat gccagtattc atattcatcg atcactcaga cggacatgtt aaaaaagcct
      721 cgctggaagc aatgagctat ggcgcggcat tggccggaaa attaggcacc tccgcggaag
      781 gtatcctcct cggcacactc ccggaagacc cttcccggtt ggggagatac gggatcacga
      841 aggttcacca ggccgcgcag gaaggcttga ataacctcga tacacaggta tatgccaaaa
      901 tcattgcaga cgccgtttcg gctgcaggcg acagcgtcgt cgtcttctcc aacaacctca
      961 gcggcaaggc agtcgcgcca cgggttgccg tccggctcaa agctgccata gtgaccggag
     1021 ccgtagcgct gcccgatatc agctccggtt ttgtcgttaa aaagaatgtc ttttccggta
     1081 aagccttcgc caatgtctcg ctcctgaccc ctatgaaggt catcgcactc aaccccaatg
     1141 catatgctat aaaggaagga gaaggtactg cggccatagt ttcactgaca gcgggtgcac
     1201 ccgctccgaa agtgaaagtc atttcggcta ctgcggcgtc gggacatgtg ccgctgacgg
     1261 aggccgaaat agtggttagc ggaggccgtg gcctcaaagg gcccgaaaac tgggggatga
     1321 tcgaagaact ggcaaaggtg ctgggtgccg cgacggcctg cagccggccg gtggccgatg
     1381 ctcactggcg cccgcacaac gaacacgtag gccagaccgg cattgccatt gcgcccaatt
     1441 tatacatcgc catcggcatt tccggcgcta tacagcatct ggcgggggtc aaccggagta
     1501 aggtcatcgt tgttattaac aaagaccccg aagcgccctt tttcaaggcg gcggattatg
     1561 gtatcgtcgg ggacgctttc gaggtcgtac ccaaaatgat cgaggcggta aagaaattaa
     1621 agggaatcaa ttgataacag cattaatcgg gccgcacgta cttataacgt ccagagtgcg
     1681 gccccttttt tgcaaaaaat ccttagtttc gcataatatt tatgaagaag atcgaactgg
     1741 aaatagtggc cttatcgcat agcatcacgc aaacccactc ctatgcagtc gtgctggggg
     1801 aggtcaacgg attgcgtcgc ctgccgatcg tgatcggggg gtttgaggct caggccatcg
     1861 ccgtagccct tgaaaaaatg caccccagcc gtccccttac gcacgatctg atgaagaact
     1921 ttatgagcgc cttcaccatc gatctgcagg agatcatcat ttgcgatctc caggaaggga
     1981 tcttctattc caaattggta tgcgtaagcg aacacgatac ggtcgagatc gactcgcgta
     2041 cctccgacgc cctcgcactg gcggtacgct tcggctgccc catcttcacc tacgaacata
     2101 ttctcgacag cgcgggcatc ctgatggaag acagctcctc caaaaagaaa aaaaccagct
     2161 cgacggcaac cgtttccacc acggaaagca gcgccaggga agaccttaag gccctttcca
     2221 tggatgacct tacctcgctg ttgaacgagg tcctggaaca ggaggattac atccgcgcga
     2281 tcgctatccg cgacgagatc aacagccggc gcaaaaccag gtgagctcac tcataaaatg
     2341 cttttttttc ccaactgcaa gatcaatctc ggattaaaga tccttcgccg ccgcgatgac
     2401 ggatttcacg atctggagac ggccttttat ccgctgccgt tgaaagatgt cctcgagatc
     2461 gtccgcacca ccgatgagac ccgcttctct tcctatggca accctattcc cggagacccc
     2521 gggtccaatc tgtgtctgaa ggcctgggat ttgctccgca acgacttccc caccctcccg
     2581 cccatccata tccatctgta taagaatata cccatcggcg cgggtctggg cgggggttcc
     2641 tccgacggag cctcgacgct gatagtactc aaccggcaat tccagctcgg tctcacgcag
     2701 caacagctcc ttgattatgc cgcccggctc ggcagcgatt gccccttctt catcctcaac
     2761 aaaccctgtc tgggaacagg ccgcggccac ctgctccaac ccctttcgct cgatctttcc
     2821 ggttattcct tcgcactcat cgatcccggt atccacatca gtaccgcaca agccttttcg
     2881 ctttgtacac cctgcgaaaa caacaccccc ctggcgacga tcctcagcca cccggtatcg
     2941 gactggcgcg aagctctgat caacgatttc gaagaaccca tcttccgcct gcatccacat
     3001 ctccgggaaa taaaggattc cctttatgac tccggagccc tctacgcctc gctcaccggc
     3061 agcggttcat cctttttcgg gattttcacg aaggatcggg cccccctgtc caaccccttc
     3121 cccgaccggt ataattatcg tatcttgcgc taaacgatcc acatctttat ccattctcac
     3181 atatcctttc tcggccggaa cctagatttc ctcgatcatc cctgtaaact ttaac
//
LOCUS       DASYSV010000408         4190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2259192, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000408 DASYSV010000000
VERSION     DASYSV010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2259192"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VGQ51_12535"
     CDS             complement(<1..576)
                     /locus_tag="VGQ51_12535"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9037450.1"
                     /translation="MSRVKRKYTTLFHICIWSILFLCIFLIRQWNHGPREGGLANSEM
                     VFALLPYIALFYVHGYWLMPAYLYRQKKHTYILMLAGLLVVVIALAGFVWHLEGLPSS
                     DASYLRSVTKRIGPAFLFLLISAGVGNFREGVRQDTIRKEKETEHLRTELSFLRTQVN
                     PHFMLNVLNSMVLLARKKSDLLEPVLLELSRL"
     gene            complement(592..1395)
                     /locus_tag="VGQ51_12540"
     CDS             complement(592..1395)
                     /locus_tag="VGQ51_12540"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEV9037451.1"
                     /translation="MKKIFIPGAVILAAVTLFSCQKTVHPDLNTASPKLVIVGEVTDG
                     PGPYTVAINRSVGFYSDNDFPAVDGATVKISDNLGVTDTLTETSPGVYTTHVLHGVTG
                     NTYTLSVTVNDTSFTAISTMPAKVLLDSVTFDHSGGRRQKSEIIPQANYQDPAGIKNY
                     YQFALYINGTQFTKDIYAFNDRLTDGRYLIQSLRMDSTYLDPGDLVKVDMYCIDVNVY
                     DYFNQLSRASGTGAFNTAAAPANPATNITGGAYGVFSAHTVSSKTVTVF"
     gene            complement(1401..3728)
                     /locus_tag="VGQ51_12545"
     CDS             complement(1401..3728)
                     /locus_tag="VGQ51_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9037452.1"
                     /translation="MQLIQKALAITLLTVPFALNAQTRHTVSGTIRDSASGETLIGAS
                     VLLEEQPQTAVSSNAYGFYSISAPAGSYHLLISFAGYLSDTIAVSLQKDIVLPVQLVV
                     AGGVLQEVVVTGNKHDNNVARPLTGVQKLSVTGIRDIPVIFGEKDILKTIQLLPGVQS
                     AGDGNSGFYVRGGTIDQNLILLDGATVYDPSHLLGFFSVFNSDAVKDVTMYKGAIPAE
                     YGGRLSSVLDVRMNDGNEKNYSASGGIGLIDSRLTIEGPIKKDDGSFVVSARRTYADL
                     FLKLSKDTNTNRNTLYFYDLNVKANYRLGEKDHLYLSGYFGNDHLGISNSFDLTYGNA
                     TGTLRWNHILGSRLFSNTSLIFSKYSYNIGINSGNNDIGIDSYIQDLHFKEDMEYYMG
                     SGNKLIFGADVIDHTISPGVIQASAASNFNSLHIPDKHSLESAVYINHDWSPTEALNL
                     DYGLRAEMFTVLGPGTFYTYDPSGNAVDSNVYAAGKTVINYFNIEPRVSMSYKLTRNS
                     SLKLSFARMTQNLHLLSNSTSANPTDVWVQNSNNVRPEIGDQVSVGYYRNLNDGRYEL
                     STELYYRYMQNQVDYKNGAQLIANTNVESQLIFGHGRAYGLELYARKKAGRLTGWISY
                     TLSRTERQFPGVNNYSWYPATQDRTHDLSVVAIYKVNKKWTLSSDFVYYTGNAITWPA
                     GKYEVDGQVAFLYTQRNGYRMPPYNRLDLSATLQGKKTQRTDSYWIFSCYNVYGRENP
                     YSISFQQDPGDPEKTQAVQYALFRWVPSISYNFKF"
     gene            complement(3873..>4190)
                     /locus_tag="VGQ51_12550"
     CDS             complement(3873..>4190)
                     /locus_tag="VGQ51_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEV9037453.1"
                     /translation="SVVDSGKNLLGQTDTKNHRNVFCAGVQYILPMLVIADLRVDMRG
                     NLRLQLDREDIPVTSRLRMNFMINTDKEYMAGFRYILTKYVGLSTHYDSDMGWGAGLT
                     FNY"
BASE COUNT          963 a         1079 c         1148 g         1000 t
ORIGIN      
        1 cagccgggac agctccagga gtaccggttc cagcagatcg gatttcttcc gtgccaggag
       61 gaccatgctg ttcagcacgt tcagcatgaa atgggggttg acctgggtgc gaaggaaaga
      121 gagctcggtc cgtaaatgtt ccgtctcctt ctctttccgt atcgtatcct gtctgacacc
      181 ttccctgaag ttgccgacgc cggcgcttat cagcaaaaaa aggaatgccg gccctattct
      241 tttcgtaacg gatctcaaat aggatgcatc ggagctcgga agtccctcca aatgccacac
      301 aaaccccgcc agggcgataa caaccactaa gagtccggct aacattagga tataggtatg
      361 ttttttttgc cggtacagat aggcaggcat caaccagtaa ccatgcacat agaacaatgc
      421 gatataggga agcagggcga ataccatttc ggaattcgcc aatcctcctt cacgcggtcc
      481 atggttccat tgccggatca aaaagataca cagaaacaat atggaccata tgcagatatg
      541 gaacagcgtt gtatattttc gtttgaccct tgacatggca acccgaattt actaaaatac
      601 cgtcaccgtc ttcgacgaca ctgtgtgcgc gctgaacacg ccataggccc cgcccgtgat
      661 attcgtggcc ggattggccg gggccgcggc ggtattgaat gccccggtcc cggatgcacg
      721 ggagagctgg ttgaaataat cgtagacatt gacgtcgata caatacatat ctactttaac
      781 cagatccccg ggatcgagat aggtgctgtc catcctcaaa ctttggataa ggtaccggcc
      841 gtcggtaaga cgatcgttaa aggcatagat atctttcgtg aattgcgtac cattgatata
      901 aagcgcaaac tggtaatagt tcttaatgcc cgccgggtcc tggtagttcg cctgcgggat
      961 gatctcgctt ttttgccggc gcccgccgct atgatcgaag gtcacggaat ccaggagcac
     1021 cttagcgggc atcgtcgata tcgccgtaaa gctcgtgtca ttgaccgtca ccgacagcgt
     1081 ataggtattg ccggtgaccc cgtgaaggac atgggtagtg tagacccccg ggcttgtttc
     1141 ggtgagcgta tccgtcactc cgaggttgtc gctgatcttt accgtcgccc cgtctacggc
     1201 ggggaaatcg ttatccgaat aaaacccgac cgaccggttg atggccacgg tatagggtcc
     1261 cgggccatcg gtgacctctc ccacaatgac cagcttcgga gacgccgtat ttaagtcagg
     1321 atgcaccgtt ttctgacagg aaaaaagggt gaccgctgca aggatgaccg cgccggggat
     1381 gaagatcttt ttcatgtcga tcagaattta aaattatagg atatggaagg tacccagcgg
     1441 aacaaagcat attgcaccgc ctgtgttttt tcggggtcgc cgggatcctg ctggaagctg
     1501 atgctgtagg ggttctctct accatagaca ttgtagcagg agaatatcca gtagctgtcc
     1561 gttcgttggg tcttttttcc ctggagcgtc gcgctgagat cgagacggtt gtaaggcggc
     1621 atgcgatagc cgttgcgttg cgtatacagg aaggcgacct gcccatccac ctcgtatttc
     1681 cccgcgggcc aggtgatggc attcccggta taatacacga aatcggaaga caacgtccat
     1741 ttcttattta ccttgtaaat ggctacgacc gacagatcat gcgttctgtc ctgggtagca
     1801 gggtaccagc tgtaattgtt gacacccggg aactgccgtt ctgtccgcga aagggtatag
     1861 ctgatccagc cggtgagacg tcccgccttt tttcgcgcat acaattcgag accgtatgcg
     1921 cggccatgtc cgaagatcag ctgcgattcc acattggtgt tggcgatcag ttgggcgccg
     1981 ttcttgtagt ccacctggtt ctgcatatac cggtaatata gctcggtcga cagctcatac
     2041 cggccatcat tgagattgcg gtaatacccg accgacacct ggtcgccgat ctctggtctt
     2101 acgttgttgc tgttctggac ccagacatcc gttgggttgg cagaagtgct gttggacaat
     2161 agatgcaggt tctgcgtcat gcgggcaaag gacagcttca atgagctgtt tcgggtgagt
     2221 ttgtagctca tcgaaacccg cggctcgata ttgaagtagt tgatcaccgt ctttcccgcc
     2281 gcgtagacat tggaatccac cgcattgccg ctcgggtcgt aggtatagaa tgtacccgga
     2341 ccaagcacgg tgaacatttc ggcacgcaga ccataatcga gattgagcgc ttcggtcggc
     2401 gaccagtcat gattgatata cacagcgctt tccagcgaat gtttgtcggg gatatgcagc
     2461 gagttgaaat tgctggccgc ggaggcctgt atcaccccgg gcgagatcgt atggtcgatg
     2521 acatccgcgc cgaagatcaa tttattcccc gagcccatat aatattccat gtcttctttg
     2581 aaatgaaggt cctggatata gctgtcgatc ccgatatcgt tgttcccgga gttgatcccg
     2641 atgttatagc tgtacttgct gaagatcagt gaggtgttgg agaaaagccg gctgccgaga
     2701 atatgattcc agcgcagcgt gcccgtcgcg ttgccatagg tgagatcgaa actgttgctg
     2761 atcccgagat gatcgttccc gaaatagccg gaaagataca gatggtcctt ctcccccagc
     2821 ctgtaattgg ccttgacgtt gagatcgtaa aaatagaggg tgttgcggtt ggtattggtg
     2881 tctttggata atttgagaaa aaggtccgca taggtacgcc gcgcgctgac gacaaaagac
     2941 ccgtcgtcct tttttatggg cccttcgatc gtcagccgcg agtcgatgag cccgatccca
     3001 ccgctggccg aatagttctt ttcattgccg tcgttcatcc tgacatcgag caccgaggat
     3061 aaccggcctc cgtattcggc ggggatcgct cccttgtaca tggtgacatc cttgaccgcg
     3121 tccgagttga aaacggagaa aaaacccagc aggtgcgacg ggtcatagac cgtcgcgcca
     3181 tccagcaata tgaggttctg atcgatggtc ccgccgcgga catagaatcc gctgttgcca
     3241 tcacccgccg actgcacgcc gggcagcagc tggatggtct tcaaaatatc cttttcgccg
     3301 aagatcaccg ggatatccct gatcccggtg acggaaagct tttgcacccc agtcaatggt
     3361 cgcgcgacat tgttgtcgtg tttgttgcct gtcacgacca cctcctgcaa tacgccgccg
     3421 gctacgacca actgcactgg aaggacgata tccttttgca gcgatacggc aatggtatcg
     3481 gagagataac ctgcaaagct gatgaggaga tgatagctgc cggccggggc actgatcgag
     3541 tagaagccgt aggcattgct cgatacggcg gtttgcggtt gctcctccag gaggacgctc
     3601 gccccgataa gggtttctcc tgatgcggaa tcacggatag tccccgatac ggtatgacgc
     3661 gtctgggcgt ttaacgcaaa gggtacggtc agcagcgtga tggcgagggc tttttggata
     3721 agttgcatgg gcgggcattg tgtatgcaag attaacgaag aaggacgtta atgaaacgtt
     3781 agggaatatc acccgccgaa atgaggatat gaagtgccgg aatgcgggta ccgctttatg
     3841 gcgcatggcc cggggcaatg gtccttgccc cctcaataat taaatgtgag gccggcgccc
     3901 caacccatgt cactgtcata gtgcgtcgac aagccgacat atttcgtcag gatataacgg
     3961 aaacccgcca tatattcttt gtcggtattg atcatgaaat tcatgcggag acggctggtg
     4021 acggggatgt cctcgcggtc cagctgcaaa cgaagattgc cacgcatgtc gacccgcaga
     4081 tcggcgatga ccagcatcgg caagatatat tggacgccgg cgcaaaacac attccgatgg
     4141 ttcttggtat cagtctgacc caaaagattc tttccgctgt ccacgacgct
//
LOCUS       DASYSV010000409         8207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1489109, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000409 DASYSV010000000
VERSION     DASYSV010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489109"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VGQ51_12555"
     CDS             <1..553
                     /locus_tag="VGQ51_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9037454.1"
                     /translation="DWLRRATTDLIFLDIHLPRLKGMSFLRTLANPPAVIITTAYHQY
                     AVEGFSLNVTDYLLKPIDFERFLVAVTKVQKQAPADAADPRDFIFVNVQKKKVKILFA
                     DIVYIESQREYIKITTTKNEYLSKMSTHEIETLLPAHLFKRVHRSYIVAIRWIESYTA
                     EMIEINGVSIPIGKGYRDSMEKL"
     gene            complement(566..1195)
                     /locus_tag="VGQ51_12560"
     CDS             complement(566..1195)
                     /locus_tag="VGQ51_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037455.1"
                     /translation="MRKLATFLIIVLAAIVIASIYSVGHDQATYSISPEYYTKFKFIQ
                     FNLADSGAAQHMTQPRSAVVMVGVKATWWMGLYIGLIIAAFALMFRNADTMFASAMQA
                     LGIVLLIAIICGVIGGYYGRYVLAKRGVGWWVPDNVVHRADFITVGSIHNFSYLGGVI
                     GLVIAIAYLFFKRARLRKRAAAAEAAAETSAETSAGTSSETAAEAEAED"
     gene            complement(1245..1412)
                     /locus_tag="VGQ51_12565"
     CDS             complement(1245..1412)
                     /locus_tag="VGQ51_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037456.1"
                     /translation="MVGATGPAGPRGQQGIHGKKGDTGTANVIYSAWSSSFSGTSQCW
                     GPITGGTTSPW"
     gene            1454..2128
                     /locus_tag="VGQ51_12570"
     CDS             1454..2128
                     /locus_tag="VGQ51_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037457.1"
                     /translation="MPDNFLTYQKFNDPELAATMCDLLRSQGIDCKIVNEAPTFDVSF
                     ANNKFEPTIHLKLPAGEFVRADVALEEYYRQQLDTLDPDYYLFAFSDEELVDIVQHPD
                     EWGALDYALAKQLLAKHGKPVIGQQEESIREQRLEELSQTEPTQPQWIFVSYLVAIVF
                     GVVGFFMGYALAFSKKNLPNGEQVFLYPPPSRRHGRRIMTISVISFCYWLWAVFSTPP
                     TLTPWI"
     gene            complement(2112..3206)
                     /gene="selD"
                     /locus_tag="VGQ51_12575"
     CDS             complement(2112..3206)
                     /gene="selD"
                     /locus_tag="VGQ51_12575"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEV9037458.1"
                     /translation="METSTIKLTSYSHGAGCGCKISPAVLDKILHSAAGKMVFPGLLV
                     GNDKRDDAAVYDLGDGKALISTTDFFMPIVDDAFDFGRIASVNAISDVYAMGGRPLLA
                     IAILGWPVDKLPPECARQVLEGARAVCAEAGIPLAGGHSIDCPEPVFGLAVNGIVPVS
                     QLKQNSTATPGCRLYLTKPLGVGILSTAGKRGFLREEDAAVALKSMTTLNRLGETFGQ
                     LEGVMAMTDVTGFGLLGHLAEMCEGSRVSAVIDFARVPVIDSVRHYIDEGCIPGGMNR
                     NWASYGEKIGLSAEMQKIGPSAEMQKTILADPQTSGGLLVAVAEGSAASFEELLRNNG
                     LPESCCRALGYLREPAPEHPLITVTSKSRE"
     gene            complement(3211..4242)
                     /gene="mnmH"
                     /locus_tag="VGQ51_12580"
     CDS             complement(3211..4242)
                     /gene="mnmH"
                     /locus_tag="VGQ51_12580"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEV9037459.1"
                     /translation="MIRRITFDELRQLPVPVPIADVRAPAEYAQGHLPGALRLPLLTD
                     EERARVGTTYKQKGREAAVLLGFEITGPKWTGYIEQAVHIAPQKKIVLHCWRGGMRSA
                     AMAWALDLYGFEVLLLQGGYKAFRHWVLRQWEKEYSLLVVGGMTGSGKTRILRQLGLL
                     GEQVIDLEDIAQHQGSSYGSINRLIQPSQEQFENQLALELSRLDPEKRIWIEDESQNI
                     GRCLIPKPLWVQMRAGLLLDVQVPLDQRVDALVGEYGSLDKDFLVECTERIRKRLGPE
                     QTTHAIAAIREHRMADFIRIVLVYYDKTYRKGVSGRPPDRIIPITLKNIEPAANARQL
                     MGAAEVSAV"
     gene            4322..4816
                     /locus_tag="VGQ51_12585"
     CDS             4322..4816
                     /locus_tag="VGQ51_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037460.1"
                     /translation="MDTTTDTSGNKRQFILEVIQPGLAGLMDGSVSSLAPLFAAALST
                     RNSHTTLLVGVATAVGAGISMAFSEALSDDGKLSGRGSPVTRGIVNGTMTFIGAAGHA
                     LPFLIHNFHLAMTVAMIIVLVELLVIAWVRHRYMDTPLLKAAFQIVIGGVIVFLAGIL
                     IGTS"
     gene            complement(4800..6149)
                     /locus_tag="VGQ51_12590"
     CDS             complement(4800..6149)
                     /locus_tag="VGQ51_12590"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEV9037461.1"
                     /translation="MGRLFFQRGSLVVGISLLVAAVAWCGYQAHHLSGEQKAIKQDYS
                     IVNNVSYGVLDVGKWRDLIVDAVRQRIEDFRLTKPEQDSLHKEIEHILNGLIDKADSM
                     MNAPQKSLKGKLRKLAYRTFVNTKSLHQQVPAFATRIMNQALRPSSKKRLAYVAKSKL
                     EQMGEDTYDSSREAEGIRLDSIYRKYGVTDLQTFNTKAQKVVETLQPRTYLYAFAMIA
                     GVLVLLGLWYLLRKSRQLHVSLYILSIAMALVLLLVGLTTTMIEIDLRMKSLDFQLLG
                     ETISFQNQVLYFQSKSIADVVMLLLRHGKYDSILVGALILCFSIFFPVTKLLATGIYL
                     LGKRSWSKNKFIYFFAFKSGKWSMADVMVVAILMAYIGFNGIIEDSLHSLNYRSNSFT
                     SITSSDTSLQPGYIIFVAFVLYGLVLSQILQWITREPLPRPAGTAGSQRPHRVSSSR"
     gene            6262..7092
                     /gene="ygiD"
                     /locus_tag="VGQ51_12595"
     CDS             6262..7092
                     /gene="ygiD"
                     /locus_tag="VGQ51_12595"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEV9037462.1"
                     /translation="MKLSSLQKITHPFGTTAPMPVLFAGHGNPMNAIEENDFVAGFRK
                     TAGMIPRPNAVLCISAHWQTKGSFVTAMEKPRTIHDFGGFPRALFEVQYPAPGSPWLA
                     EETRNLVRSTELGLDDRWGLDHGAWSVIKHFYPMADIPVIEMSLDYSQPAQYHYDLAK
                     ELAPLRRKGVLIMGSGNMVHNLRMVAWDRLETTFGFDWALGAAQKMKDAILAGDHRPL
                     IDYSSQGKEFDLAIPTPEHFLPLLYALALKEEKEPVSLFNDQPVGGSITMTSVRIGGE
                     "
     gene            7289..>8207
                     /locus_tag="VGQ51_12600"
     CDS             7289..>8207
                     /locus_tag="VGQ51_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEV9037463.1"
                     /translation="MIPRIVSPIIIALPALLTVQLLSAQFRTEKDLLGEKQIPADAYY
                     GVQTARALENFQVSGVATKFYPDYVRAYAMVKLAAARANAEVGRMSQDKLTAIEKACQ
                     AVIDGKYHEHFLVDLYQGGAGTSANMNANEVLANIALELSGHKKGEYNIVEPHDDLNM
                     GQSTNDVYPTAIHVALLLQNDKVVKEAKDLSAAFHQKGEAFKNILKMGRTEGQDAVPM
                     TVGQEFHAFGNQLDAAIEALIGSEAYLYEENMGATAIGTGITASPGYAEKCAAQLARI
                     TGKPMVLSKDLIAATSSMQAFVLYSAALKN"
BASE COUNT         1876 a         2371 c         2138 g         1822 t
ORIGIN      
        1 cgactggcta cggcgcgcca ctaccgatct catcttcctg gacatacacc tgccacggct
       61 gaaagggatg tcctttctgc ggacgctggc caacccaccc gccgtcatca ttactaccgc
      121 ttatcaccaa tacgcggtgg agggatttag cctgaatgtc acggactatc ttctgaagcc
      181 gatcgatttc gaacgtttcc tggtcgcggt gaccaaagtg cagaagcagg cgccggcgga
      241 tgcggccgat ccccgggatt tcatctttgt caacgtgcag aagaaaaagg tgaagatatt
      301 attcgcggac atcgtttata tcgagagcca gcgggaatat atcaagatca cgaccactaa
      361 gaatgaatat ctttctaaaa tgagcacgca tgagatcgag acactccttc ccgcgcatct
      421 gttcaagcgg gtacaccggt cgtatattgt ggcgatccgg tggatagagt cttatacggc
      481 cgaaatgatc gagatcaatg gcgtcagcat tcccatcggg aaagggtatc gggatagtat
      541 ggaaaaattg tgagaagcgg tcttctcagt cctcggcctc cgcctcagcc gccgtctcgg
      601 aggatgtccc ggctgatgtc tccgcagacg tctcggctgc tgcctccgct gcagctgccc
      661 gttttcgcaa ccgcgcccgc ttgaagaaga gatatgcgat ggcgatcacc agcccgatca
      721 cgcctcccag atagctgaaa ttgtggatcg agccgacggt gatgaagtcg gcgcgatgca
      781 ccacgttatc gggcacccac catccgaccc ctcttttggc cagcacataa cgaccataat
      841 aaccgccgat caccccgcaa atgatcgcta tcagcaacac gatccccagg gcctgcatcg
      901 ccgatgcgaa catcgtatcc gcattccgga acatcagcgc aaaagccgcg atgatcagtc
      961 cgatatacag ccccatccac cacgtggcct ttacccctac catgaccacc gcgctgcgcg
     1021 gctgcgtcat atgttgcgcc gctcccgaat ctgcgaggtt aaactggata aatttgaatt
     1081 tcgtataata ttcggggctg atagaataag tcgcctggtc atggccgacg ctatagatcg
     1141 aagcgattac gatcgccgcc agcacgatga tgaggaaggt agccaatttt cgcatatctt
     1201 aaagatacga acaatccggc cctaaggctg gaatttcaac agtttcacca tggactggta
     1261 gtcccgccgg taatcggtcc ccaacactgc gaagttcccg aaaagctgct gctccaggcg
     1321 gaataaatga cattggcagt gccggtgtcg cccttctttc catggatacc ctgctgtccc
     1381 cggggacccg ccgggccggt tgcacccacc actttccggg gatatcttat ctttacgctt
     1441 tcaatgcgtc tacatgccgg acaatttcct gacctaccaa aaattcaacg accccgaatt
     1501 ggcagccacg atgtgcgacc tgctccgctc gcagggcatc gactgcaaga tcgtcaacga
     1561 agccccgacc ttcgacgtga gtttcgccaa taacaagttc gagccgacca ttcatttgaa
     1621 actgcctgcc ggcgaatttg tccgtgcgga tgtggctctt gaagagtact accggcagca
     1681 gctcgatacc ctggaccccg attattacct gtttgctttt tccgacgaag aattggtgga
     1741 tatcgtacag catcccgacg aatggggcgc gcttgattat gcgcttgcca aacagctgct
     1801 cgccaaacac ggcaagccgg tcatcggcca acaggaagag agcatccggg aacaaagact
     1861 tgaggagttg tcacagacag agcccacgca acctcagtgg atctttgttt cctatctcgt
     1921 ggccatcgtt ttcggagttg tcggtttttt catggggtat gcgcttgctt tttcgaaaaa
     1981 gaatctgccc aatggcgaac aggtttttct ctaccctccg ccctcacgcc gtcatggtcg
     2041 acggatcatg accatctccg tcatatcgtt ttgctactgg ctgtgggccg tcttttcgac
     2101 gccgccgacg cttactccct ggatttagat gtgacggtga tcaatggatg ttccggcgcc
     2161 ggctcgcgga gatatcctaa tgcccgacag caggattcgg gcaacccgtt attacgcaac
     2221 aattcttcga aagacgcggc actcccttcg gctaccgcca ccaaaagccc tccgctcgtc
     2281 tgagggtcgg caagaatggt cttttgcatc tccgcggagg gcccgatctt ttgcatctcc
     2341 gcggaaaggc cgatcttttc cccatagctg gcccaattgc ggttcatccc gccgggaatg
     2401 caaccttcat cgatataatg cctcacagag tcgatcactg gcacccgcgc aaaatcgatc
     2461 actgccgaca ccctgctgcc ttcgcacatc tcggcaagat gtcccagcaa cccgaatccg
     2521 gtgacgtcgg tcatggccat gacgccttcc agttgcccga atgtctctcc cagcctgttg
     2581 agggtcgtca tgcttttaag cgcaaccgcg gcatcttctt cacgcaaaaa ccctcgtttt
     2641 ccggcggtgg acaagatgcc gacgcctagt ggctttgtca gatagagacg gcaacccggg
     2701 gtcgccgtag agttctgttt tagttgcgat accggcacga tcccattcac ggccaggccg
     2761 aataccggtt cgggacagtc gatgctatgc ccgcccgcca gcggaatgcc tgcctctgcg
     2821 cagacggccc ttgcgccttc cagtacctgc cgggcgcatt cagggggcag cttatccacc
     2881 ggccatccga ggatggcgat agccagcaat ggtctacccc ccatcgcata gacatcactg
     2941 atggcattga ccgatgcgat acggccgaaa tcaaaagcat catccactat cggcataaag
     3001 aaatccgtag tcgagatcag ggctttgccg tccccaagat cgtaaacggc cgcatcgtcg
     3061 cgtttgtcat ttcccacgag caatcccggg aacaccatct tgccggcggc gctatgcaat
     3121 atcttgtcca ggacggccgg gctgatcttg caaccgcaac cggcgccgtg tgaatagctg
     3181 gtcagtttta tcgtcgaagt ctccatatat ttaaacagcg ctgacctccg ccgctcccat
     3241 cagttgtcgg gcattggcgg ccggttcaat attttttaag gtgatgggga tgatgcgatc
     3301 cgggggcctg ccgctgaccc ctttcctata agttttatca taatagacga gcactatccg
     3361 gataaagtcc gccatccggt gttcccgaat ggctgcaatg gcgtgagtgg tttgctccgg
     3421 gcccaaccgc ttgcgtatcc gctcggtaca ttccacgagg aaatccttgt ccagcgaccc
     3481 atattcgccg acaagagcat cgacacgctg gtccagcgga acctggacat ccaggagcag
     3541 gccggcccgc atttgtaccc acagcggttt cgggatcaaa caccgcccga tattctggct
     3601 ttcatcctcg atccagatcc tcttttccgg atcgagacgg gacagctcca gggccagttg
     3661 gttctcgaac tgctcctggc tgggttgtat caaacggttg atcgatccat aggacgatcc
     3721 ctgatgctgg gcaatatctt cgagatcgat gacctgctct cccaaaagac ccagctgtct
     3781 caaaatgcgt gtcttgcccg agcctgtcat gcctcccacg acgagcaacg aatactcctt
     3841 ttcccactgc cgcaataccc aatgacggaa ggccttatat ccgccctgca gcaggaggac
     3901 ctcgaaaccg taaagatcga gcgcccaggc catggcggcg ctacgcatcc ctccgcgcca
     3961 gcagtgaagg acgatctttt tctgcggcgc tatatggacc gcctgttcga tatagccggt
     4021 ccatttgggt ccggtaatct cgaagcccag taaaacggcg gcttcccgac ccttttgttt
     4081 ataggtggtc cccacgcggg cgcgctcttc gtcggtcaac agcggcagac gcaaggcccc
     4141 cggaagatgt ccctgggcat attcggcagg tgcgcgcacg tcggcaatgg gaaccggtac
     4201 gggcaactgt cgcaactcat cgaaggtgat acggcggatc atgaagtccg gtcgccgacc
     4261 ggccggcaaa tatacgaagc tgccggaaat gtcttaattt aaggcgactt aacaattgca
     4321 gatggatacc acgacagaca catcggggaa taaacggcaa ttcatattgg aggtgatcca
     4381 gccgggtctt gccgggctga tggacggatc cgtttcctcg cttgcgccct tgtttgcggc
     4441 agcgctctcc acccggaata gccatactac tctcctggta ggtgtggcga ccgccgtcgg
     4501 ggcgggtata agtatggcct tttcggaggc gttgtccgat gacggcaaac tcagcggaag
     4561 gggaagcccg gtcacccgcg gcatagtcaa cgggaccatg acctttatcg gtgcggcggg
     4621 tcatgcccta cccttcctga tccacaattt ccacctggct atgacggtcg cgatgatcat
     4681 cgtactggtc gagctgttgg tgatcgcctg ggtgagacac cggtatatgg ataccccgct
     4741 gctgaaggct gcgttccaaa tcgtcatcgg tggcgtcatc gtatttttgg ctggcatcct
     4801 tatcgggacg agctgacgcg atgagggcgc tgcgagcctg cggtacctgc gggccggggc
     4861 agtggttcac gcgttatcca ttgcagtatc tgcgaaagca ccaggccata gagcacaaac
     4921 gccacgaaga tgatataacc gggttgcagc gaagtatcgc tgctcgtgat gctcgtaaag
     4981 ctgttcgacc ggtagttgag tgagtgtaga gagtcctcga tgatgccgtt gaacccgata
     5041 taggccatca atatcgccac taccatgacg tcggccatgc tccatttacc ggacttgaag
     5101 gcaaaaaaat aaatgaactt gtttttcgac caggagcgtt ttccaagaag atagatcccg
     5161 gtggccagca atttggtgac ggggaagaaa atgctgaaac acaggatcag cgcccctacc
     5221 aggatcgaat cgtatttacc atgacggagc agaagcataa ccacgtcggc gatgcttttg
     5281 ctttggaaat aaaggacctg gttctggaag gaaatggtct cgcccaatag ctgaaaatcc
     5341 agggacttca tacgcagatc gatctcgatc atggtcgttg tgagccccac cagcaacagg
     5401 accagggcca ttgcgatcga taggatatac agggaaacat gcagctgtcg ggatttgcgc
     5461 agcagatacc acaatcccag caacacgagt acgccggcga tcatggcaaa agcatatagg
     5521 tacgtccgcg gctgcagcgt ctcgaccacc ttctgcgctt tcgtgttgaa tgtctggagg
     5581 tcggttacac catatttccg gtaaatggaa tccagtctta taccttccgc ctcgcgtgag
     5641 ctgtcatagg tatcctcccc catctgttcc agcttgcttt ttgccacata agcgagtctt
     5701 tttttgctgg aaggacgcag cgcctgattc atgatccggg tcgcaaacgc cggcacctgc
     5761 tgatgcaggc ttttggtgtt gacgaaggtc ctgtatgcca gctttcgcaa tttacccttc
     5821 aaggattttt gcggggcgtt catcatgctg tccgctttat cgatcaatcc gttcaggata
     5881 tgctcgatct ccttgtgaag cgaatcctgt tccggcttcg tcagtctgaa atcttcgatc
     5941 ctttgccgca cggcatccac gatcaggtcg cgccatttgc ccacatccag cacgccatag
     6001 ctgacattgt tgacgatgct gtaatcctgt ttaatggcct tttgttcacc cgaaagatgg
     6061 tgagcctggt atccgcacca ggcgacggca gcaacgagta aggatatccc tacgacaagc
     6121 gatccccgtt ggaagaacaa tctgcccata ttatggtata ggcgaattct gtgccacggg
     6181 gcgcggctcg cgcgcaatcg attgatactg tggcaacatc gccggattgg gcacacctga
     6241 ataagccgta attttgtgcc ggtgaagctg agctctctgc aaaagataac ccatccattc
     6301 gggaccactg ccccaatgcc cgtcctgttc gccggacacg gcaaccccat gaatgccatc
     6361 gaagagaatg acttcgtggc cggctttcgc aagacggccg gtatgatccc tcgtccgaat
     6421 gcggtgcttt gcatttccgc gcattggcaa acgaagggca gcttcgtgac tgcaatggaa
     6481 aaaccgcgca ccatccacga tttcggcggg tttccgcggg cgctgttcga agtgcaatat
     6541 cccgctccgg gaagcccctg gctggcagaa gagacccgaa atctcgtccg atcaaccgaa
     6601 ttgggactag acgaccggtg gggcctcgat catggcgcat ggtcggtcat caaacatttc
     6661 tatccaatgg ccgacatccc cgtcatcgag atgagcctcg actacagtca gcctgcacaa
     6721 taccattatg acctggcaaa agaattggcg cccctgcgga ggaaaggcgt attgatcatg
     6781 ggcagcggca atatggtcca caatctacgt atggttgcgt gggacagact ggaaacaact
     6841 ttcggtttcg actgggcgct cggggcggcc caaaaaatga aagacgcgat cctggcgggc
     6901 gaccaccgcc cgctgatcga ttattcttcg caaggaaaag aattcgatct cgccattcca
     6961 accccggagc attttctgcc cttgctctac gcactcgcac ttaaagaaga aaaagaaccg
     7021 gtcagcctct tcaacgatca acccgtaggc ggatcgatca cgatgacgtc ggtgcgcatc
     7081 ggcggcgaat agctctcacg cgcgatactc acgcgccaca ctcgcgcgat atacaagcgc
     7141 gacactcacg cgacatttac atcccgggga tctccttgta agcttgccaa atcttacgag
     7201 tcttggcatc tatgccactg cgcccgcgtg ttcacgtcaa aattgtaaag acttttgctc
     7261 agcgttcgat cacccaaact tttacattat gattcccagg atcgtttccc ccatcatcat
     7321 tgcactgcct gctttgttaa ccgttcaact tctgtccgcc caatttcgaa ccgaaaagga
     7381 tcttctcggt gaaaaacaga tccccgccga tgcgtattac ggcgtgcaaa cggcacgcgc
     7441 cctggaaaat ttccaggtca gcggtgtggc taccaaattt tatcccgatt atgtccgcgc
     7501 ctatgcgatg gtgaaattgg ccgccgcccg tgccaatgcg gaagtgggtc gtatgagcca
     7561 ggacaaactc actgctatcg aaaaggcttg tcaggccgtg atcgatggca agtaccatga
     7621 gcatttcctg gtcgacctat atcagggcgg cgcgggaacc tcagccaata tgaacgccaa
     7681 cgaagtgctc gccaatatcg cgctggagct cagcgggcat aaaaagggag aatacaatat
     7741 cgtcgagccg cacgacgatc tcaatatggg tcaatccacc aacgacgtct atcctacggc
     7801 catacacgtg gcgctgctgc tccagaacga taaagtagta aaagaagcga aagatctgtc
     7861 cgctgcattt catcaaaaag gcgaggcatt caaaaatatc ctgaagatgg ggcgtacaga
     7921 agggcaggac gccgttccca tgactgtcgg ccaggaattt cacgcattcg gcaaccaact
     7981 cgatgcggcc atcgaagcgc tgatcggatc ggaggcttat ctatacgaag agaatatggg
     8041 ggccaccgcc attggcactg gtatcaccgc ctcaccggga tatgccgaaa aatgcgccgc
     8101 gcagctggca aggatcacag ggaaacccat ggtgcttagt aaagacctga tcgcagccac
     8161 ctccagcatg caggcgtttg tcctatattc ggcggcgctg aaaaatc
//
LOCUS       DASYSV010000410         6416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_82161, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000410 DASYSV010000000
VERSION     DASYSV010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82161"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VGQ51_12605"
     CDS             complement(<1..1316)
                     /locus_tag="VGQ51_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEV9037464.1"
                     /translation="MNAAKRRLFLAGACWLMVSLLRAQDKLPVKFGKVTPDDFKVSAP
                     ATDPDAGAVVIADFGTSEFEGNSTGGFEILFKHSRRIRILKRNAFEAATVTIPLYVSN
                     HEQERIEKLSAATYNLEDGKVVETKVGDKSIFVDKLNRNHIQKKFTFPALKEGSILEY
                     TYTQTSPFIFNLQPWAFQSQYPCLWSEYQADMPEFFRYVTLSYGFVPFSIKTTESRFQ
                     RFNLNIGNGSLRSIDGEVVTHRWVMKDIAALKEEPYTTSLYNYLARIEFQLSALQFTQ
                     VNYYKDYIGDWFKLSEGLLTDEYFGADLDRGNGWMDDDCKAVTQGAKSELEKAKRIFA
                     FVRDKFNCTSHSSLYLSEPLKTTFNNRNGSEADMNLLLTAMLRHGKITADPVILSTRA
                     HGFANEVYPLLDRFNYVITRVRIDSSEYFLDASEPWLGFGRLPERC"
     gene            complement(1322..3358)
                     /locus_tag="VGQ51_12610"
     CDS             complement(1322..3358)
                     /locus_tag="VGQ51_12610"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEV9037465.1"
                     /translation="MTRLKPIAHPAIVLCLCLVVFPSFAQDKAKNTLGKVTPSDFNLP
                     NLPITDSNTTAVILADKGETHYIGNRTGWFSCVYTRQTRIKVIDKKAFDLATVKIYLD
                     GQQKEMEKLSNVSASTFNLGQRQISQTKLDEKDIFRNKLDKDRTEVKFTLPAVKEGSI
                     IEYSYTITSDYWWIMPPWEFQFRKYPCLYSEYQVEIPQTISFVLVRQGVHPYAVDNGS
                     IGRVSYTVTVNPDKSSLANLDQDAYVSANTVKHDWVMRDIPAFGNEPYLTTPENYIDK
                     IAFQLSGTHNNSEESTDYHNNWAKATDELLGQERFGGALEQDDNRVDEFADKIGVGDA
                     DRLALAKAVYYYMSQHFTCTENWRDITTTLPDVIRKGGGTVGDINLLLIAVLRKKGFF
                     AEPVVLSTRDHGFNFVHYPILERLNYVIVRLILDGRTYYLDAAHPELGFGQLADECYN
                     GPARIISKQDSGSVYFDADSLKERSTTLVMMTSTDKGLEGSWQSTLGTQQSYETRKNV
                     REHGQQQFFKDIQTQYGDDLAITNGGIDSLSLPEEPVKVHYEFVLKQQPGLSLIYLSP
                     MLGDAWRKNPFEAAERKYPIELPYSMDENYIFSMDIPQGYAVDELPKSARVGLNGDQG
                     QFEYLIAQQGDQIQMRCRLRLNKAWFPATDYSSLRDFFGYVVKKEAEQIVLKKK"
     gene            3510..4430
                     /gene="queG"
                     /locus_tag="VGQ51_12615"
     CDS             3510..4430
                     /gene="queG"
                     /locus_tag="VGQ51_12615"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEV9037466.1"
                     /translation="MAKAVRLDDDARRLEEWLGKGMQGTMHYMENHFDLRIDPSRLVP
                     GARSVITLLMNYFPPVQQPAEVPRIAKYAYGEDYHEVIRAKLNRLLENMRSAIGEIDG
                     RGFVDSAPVLERSWAVRSGLGWIGRNGNLLNRKAGSFFFIATLIVDIPLEYDAPFAGD
                     YCGTCRRCIDACPTEAISDDRVVDGSKCISYFTIELKDQLIPDAMKGRFDDWLFGCDT
                     CQDVCPWNRFARPAAEPAFTPLPAILNFRTRDWEELTEEKFREIFRHSAIKRTKWAGL
                     RRNLSFISGPAEPPAGHLHKKSADPPDPDH"
     gene            complement(4354..5763)
                     /locus_tag="VGQ51_12620"
     CDS             complement(4354..5763)
                     /locus_tag="VGQ51_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037467.1"
                     /translation="MYDPRFEKAVQHKMEELEIRPSESVWVNIEKTVSAERRRRGAPF
                     FWRLFIATMLVGGVVTGYYFGEKAGRRSVAPATARAGTAGGAKASASDPSGPPETRNK
                     QVEVSKTPAAASAASKKKYTRTTTSDDSVIHATIVSKQTAEERFADEAKTSADEAKTS
                     SDEAKTSSDESKTSSDEAKTSVTGNSRIGAYLYTPGLEEQRYGVRVQGASLKTNKNMV
                     SLNTLSQVRRPWEAGFVAGGGFDRLNRLDGGQAGNAASYTASSFYGLTRAATKTDISD
                     VRPGASFYAGIYLQKQVSNRWTFHTGLDLHYYSTSVSIGQQLNVIASPAASLLNQTAL
                     AAAPAPLVFTVGDLQNFVNRYYLLELPVSMQYRINRSKLLPLFVEGGAAVSRLMGANA
                     IFYNPSTGLYLKNENVLNKTQFNISSALLVGLPFHGIRIQAGPEVQYGLTPLINGQGL
                     GDQHFFYAGVRLVVLPGRK"
     gene            complement(5750..6313)
                     /locus_tag="VGQ51_12625"
     CDS             complement(5750..6313)
                     /locus_tag="VGQ51_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV9037468.1"
                     /translation="MDHHQLVRDCLRGDGTAQRQLYEQFAGAMLGVCYRYTKSLVDAE
                     DVLQEGFIMVYKNLHQFNFAGELGGWIRRIMVNTAINYLKKNSRYQTDLLYTDGVLHP
                     VSDDDPEVSLSTKELAELIRQLPAGYQTIFNLHAVEGYSHVEIGKILGIKEGTSRSQY
                     ARARSLLISWINRFEAANKNETTYVRP"
BASE COUNT         1420 a         1777 c         1629 g         1590 t
ORIGIN      
        1 taacatcttt cgggcagccg tccaaaaccc agccagggtt cgcttgcatc gagaaaatat
       61 tcagacgaat cgatcctgac ccgggtgatc acataattga accggtccag taagggatag
      121 acctcattgg caaaaccatg cgccctcgtg ctgaggatca ccgggtcggc ggtgatcttc
      181 ccgtgacgta acatggccgt caatagcagg ttcatatcgg cttcgctccc gttcctgtta
      241 ttgaatgtgg tcttcaatgg ttccgacagg tacaggctac tatgcgaggt acaattgaat
      301 ttgtcccgca cgaacgcgaa gatccgcttc gccttctcca actcgctttt cgctccctgg
      361 gtcacggctt tgcaatcatc gtccatccag ccgttgcccc tgtccagatc cgctccgaaa
      421 tattcgtcgg tcagcaatcc ctcgctcagc ttgaaccagt ctcctatata atccttatag
      481 taattgacct gagtgaattg aagcgccgaa agctgaaact cgatcctggc caggtaattg
      541 tacaaagagg tggtataggg ttcttccttc agcgccgcaa tgtccttcat gacccatcga
      601 tgggtgacca cctcgccatc gatgcttcga agacttccgt tcccgatgtt caggttgaac
      661 cgctggaatc ggctctcggt ggtttttatg ctgaagggca cgaaaccata gctcagcgtg
      721 acataccgga aaaattcggg catgtcggcc tggtattcgc tccacagaca cggatactgg
      781 ctctgaaagg cccagggttg aagattgaaa ataaacggtg aagtctgagt ataggtgtac
      841 tccaggatcg atccttcttt tagagccgga aaagtaaact tcttttgaat atggttccgg
      901 ttcagcttgt cgacgaatat ggatttgtcg cccactttgg tctctacgac cttcccgtcc
      961 tccaggttgt acgttgccgc actcagcttt tctattcttt cctgttcatg attactgaca
     1021 tacagcggta tggtgaccgt tgcggcttcg aatgcgttcc gtttcaaaat cctgatccgc
     1081 cgggaatgtt tgaagagaat ctcgaatccg cctgtggaat tgccttcgaa ctcgctggta
     1141 ccgaagtcgg cgatgacgac ggctccggcg tccgggtcgg tagcgggtgc ggaaaccttg
     1201 aagtcgtcag gagtcacctt cccgaatttg acaggaagct tatcctgcgc ccgcagcagc
     1261 gataccatca gccaacaggc gccggctaaa aacaatcgtc tttttgcagc gttcatggtc
     1321 ttcatttctt tttcaatact atctgctcgg cctctttctt cacgacatat ccaaaaaaat
     1381 cccggagact ggaataatcg gttgccggga accaggcctt gttcagcctg agccggcatc
     1441 gcatctgtat ctgatcaccc tgctgcgcga tcaggtattc gaattgcccc tggtcgccgt
     1501 tgagacccac cctggccgat tttggcagct cgtccaccgc ataaccctga ggtatgtcca
     1561 tcgagaaaat atagttctcg tccatggaat aaggcagctc gatggggtat ttcctttcgg
     1621 cggcttcgaa ggggttcttg cgccatgcat ccccgagcat cgggctcaga tagatcagcg
     1681 ataagccggg ttgctgtttc aggacaaatt catagtggac cttgacaggc tcttccggca
     1741 ggctcagcga gtcgatcccc ccgttggtga tcgctagatc gtctccatat tgcgtctgga
     1801 tgtccttgaa gaattgctgt tgtccatgct cgcggacatt cttccgcgtc tcataggact
     1861 gctgtgtccc cagcgtggat tgccagctgc cttccaatcc cttgtccgta ctggtcatca
     1921 tcaccagggt ggtgctgcgt tctttcagcg agtcggcatc gaaatagacc gagccggaat
     1981 cctgtttgct gataatgcgt gcagggccat tgtaacactc atcggccaac tgcccgaatc
     2041 ccaactcggg atgcgcggcg tcgagataat aggtcctgcc gtccaggatc agccggacga
     2101 tgacatagtt cagccgttcg agaatgggat aatgcacgaa gttgaatcca tgatcccttg
     2161 tactaagcac taccggttcg gcgaaaaaac ctttttttcg caggacggcg atcagtaaca
     2221 ggttgatatc cccgaccgta ccgccgcctt tccggatgac atccggcaat gtcgtagtga
     2281 tatccctcca attttcggtg caggtgaaat gctggctcat gtagtaatag acagcctttg
     2341 ccagggcaag cctgtcggca tctccgacgc cgatcttgtc cgcgaattca tccacccggt
     2401 tgtcatcctg ttccaacgcg ccgccaaacc gctcctgccc caacagctca tcggtagcct
     2461 ttgcccagtt attgtggtag tcggtggact cttccgaatt attatgggta ccggaaagct
     2521 ggaaagcgat cttatcaata taattttcgg gcgtagtgag gtaaggctca ttcccaaagg
     2581 cgggaatatc cctcatgacc cagtcatgtt tgacggtatt ggccgacacg tatgcatcct
     2641 ggtccagatt ggcaagcgag cttttgtccg gatttaccgt taccgtataa cttacacgac
     2701 cgatactgcc attgtcgacg gcatagggat gcacgccctg acggacgagg acaaaagaaa
     2761 tggtttgcgg gatctccacc tggtactcgc tgtacagaca cggatacttc ctgaattgga
     2821 attcccacgg cggcatgatc caccaataat cggaagtaat cgtatagctg tattcgatga
     2881 tgctgccctc ctttacggca ggcaacgtga attttacttc ggtccggtcc ttgtccagct
     2941 tgttccggaa aatatccttt tcgtcgagct ttgtctggct gatctgacgc tggccgaggt
     3001 tgaaggtgga ggctgacaca ttgctaagtt tttccatctc tttctgctga ccgtcgaggt
     3061 atatctttac ggtggccaga tcgaatgcct tcttatcgat gacctttatc ctcgtctgcc
     3121 gggtatagac acacgagaac cacccggtcc tgttcccgat gtaatgcgtt tcgcccttgt
     3181 ccgcaaggat cacggcggtg gtattgctgt cggtgatggg aagattcggg agattgaaat
     3241 ccgaaggagt gacttttcct aatgtattct tagctttgtc ctgggcgaag gatgggaata
     3301 cgaccaggca taggcatagg acaatcgcag ggtgtgcaat cggtttgagg cgtgtcatag
     3361 taggcgttaa gggttgaccg ccgacaagat agacaaatta cccagaaacc caataccctc
     3421 acagggtaaa aaatcaacat ctgacagaaa aagaccatac atattggatc aaaacctgga
     3481 gcagcgagct tggtttcgat cactgcggta tcgcaaaggc cgttcgtctc gacgacgacg
     3541 cccgccggct cgaagagtgg ttgggtaaag gcatgcaggg tacgatgcac tacatggaga
     3601 atcatttcga tctgcggatc gatccttccc ggctggtgcc gggagcaaga tcggtcatta
     3661 cgctgttgat gaattatttt cctccggtac aacagcctgc ggaggttccc cgtatcgcca
     3721 aatatgccta cggcgaagat tatcatgagg tcatacgtgc caagttgaac cggctgctgg
     3781 aaaacatgcg ctcggcgatc ggagagatcg acggccgcgg gttcgtcgat agcgctcctg
     3841 tactggaacg cagttgggca gtacgcagcg gtcttggctg gattggcagg aatggcaatc
     3901 tgctcaaccg gaaggcgggc tcattcttct ttatcgccac cctgatcgtg gatatcccgc
     3961 tggagtatga cgcgcctttc gcgggtgatt attgcggtac ctgtcgtcgc tgtatcgacg
     4021 cgtgtcctac ggaagccatt tccgatgatc gcgtcgtgga cggcagcaaa tgcatctcct
     4081 attttaccat cgagctgaaa gatcagctga tccccgatgc catgaagggg cgtttcgacg
     4141 attggctttt cggatgcgat acctgtcagg acgtgtgtcc gtggaaccgt tttgcaagac
     4201 ccgccgccga accggccttt acgccattgc cggcgatcct gaattttagg acacgcgact
     4261 gggaagagtt gacggaagaa aaattccggg agatattccg gcattcggcg atcaagcgta
     4321 ccaaatgggc cggactacga cgcaatctga gttttatttc cggcccggca gaaccaccag
     4381 ccggacacct gcataaaaaa agtgctgatc ccccagaccc tgaccattga tcagcggggt
     4441 cagaccatat tgcacctccg gtcctgcctg gatgcggata ccatggaaag gaagcccgac
     4501 cagcaatgcg gaggatatgt tgaattgcgt cttgttgagt acattttcat tcttcagata
     4561 aagacccgtc gaagggttgt agaaaatggc attggcgccc atcagcctcg aaacggcggc
     4621 gcctccctcc acgaacagcg gcaacagctt gctccggttg atcctgtatt gcatcgatac
     4681 cggcagctcc agcaggtagt aacggttgac aaaattctgc agatcgccta ccgtgaacac
     4741 caacggtgca ggcgcggcgg caagcgcggt ctggttcagc agagaggctg cgggcgaagc
     4801 tatcacattc aattgctgtc cgatgctcac gcttgtgcta taatagtgaa gatccaaccc
     4861 ggtatgaaag gtccatcggt tcgaaacctg tttctgcaga taaatgccgg cataaaaaga
     4921 agcgcccggt cgcacgtccg aaatatcggt ctttgtcgct gcccgtgtca gaccataaaa
     4981 ggaggatgcc gtataggatg cggcattccc cgcctggccg ccatccagac gattgagacg
     5041 atcgaatccg cctcccgcga caaaccccgc ctcccagggt cgtctcacct gcgatagggt
     5101 attcaaagag accatgttct tattcgtttt caacgaagca ccctgaaccc ggacaccata
     5161 ccgctgttct tccagacccg gtgtataaag atatgccccg atgcgggaat tgccggtcac
     5221 cgatgtcttc gcctcatctg acgatgtctt cgactcatct gacgatgtct tcgcctcatc
     5281 tgacgatgtc ttcgcctcat ctgcagacgt cttcgcctca tctgcaaacc tctcttccgc
     5341 cgtctgcttc gaaactatgg tcgcatgtat cacagagtca tcgctggtag tcgttctggt
     5401 atatttcttt ttcgaagctg ctgacgctgc cgccggcgtc ttcgatactt ccacctgttt
     5461 gttccttgtt tcgggcggtc ccgacggatc ggaagcggac gctttagccc ctccggctgt
     5521 cccggcccga gcagtcgccg gcgccacaga tctcctcccg gccttctccc cgaaataata
     5581 tcccgtgacg acaccgccaa cgagcatggt ggctatgaac agccgccaga agaacggcgc
     5641 gccacgccgg cgacgctccg cagaaacggt cttttcgata tttacccata ccgactccga
     5701 agggcggatc tccagctcct ccatcttgtg ctgaactgcc ttctcaaatc tagggtcgta
     5761 cataggtcgt ttcattttta ttcgcagctt caaatctatt tatccagctg atgagcaggg
     5821 accttgcccg cgcatactgg ctacgggacg tcccttcctt gatgcccaat atctttccta
     5881 tctccacgtg actataacct tcgacggcat gcaggttgaa aatggtctgg tatcccgccg
     5941 gcaattgcct gatcagctct gccagctcct tggtgctcaa agaaacctcg gggtcatcgt
     6001 cgctgacggg atgaaggacg ccgtccgtat agagcaggtc ggtctgatac cggctgttct
     6061 tcttgagata attgattgcc gtattgacca tgatccggcg gatccacccc cccagctccc
     6121 cggcaaaatt gaactggtgc aaattcttgt aaaccataat aaacccttcc tgcagcacat
     6181 cttccgcatc gaccagggat ttggtatagc gatagcacac ccccagcatg gcgccagcga
     6241 attgctcata gagctgccgc tgagcggtcc catcccccct gagacaatcc ctgaccaact
     6301 ggtgatgatc cattttcctt tattgaattg gttgacaatt ataatcgccc gttcgttgca
     6361 tcatccccta cctttacact atgataccct cactcgccga acgcgatgga caaaat
//
LOCUS       DASYSV010000411         5715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1364249, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000411 DASYSV010000000
VERSION     DASYSV010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364249"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VGQ51_12630"
     CDS             <1..1022
                     /locus_tag="VGQ51_12630"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV9037469.1"
                     /translation="RFSLFMIPLLFCLTVAAQSNFAAVDQLLKQNQKTLANFVVLVSK
                     DGKIVYQKQGGTDFTAKMQAPIAGSGDWMTAALVMTFVDEGKLSLDDKVTRYIPLFGK
                     YMKGYITIRNCLTNTTGIKTDEAVEKPMERNHFETLEDMVNTYANKHAIATNPGTEIF
                     YSNLGPNIAGRILEIVSKKAFDRLMRERITNPLKMRGTSFTNEEGGATNPSNGARSTA
                     NDYINFMTMLLNKGVFEGKRILSEKAVAEIETAQFPSLPVKFMPAGLQGAHMGLGCFI
                     TNTGSSGAPTAFVSHSLLGAAAFMDTCRNYSAIIIVEKPQEEKKPLYQNLMNLIGEAI
                     GGACN"
     gene            complement(1079..2980)
                     /locus_tag="VGQ51_12635"
     CDS             complement(1079..2980)
                     /locus_tag="VGQ51_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEV9037470.1"
                     /translation="MRSILYWLLLLAPGLASAQNYSTALIPDSLRKGARAVVREDELI
                     LEIKSPAKAVEREHEVYTVLNPNGDNLGGIMVGYDKFESIRDISGVLYDSLGKVVRKA
                     KRKDMQDKGYVDESSLASDDRYLEHNFYCQVYPYTVDYQKEIEHDGIFGFDDWLPLAT
                     SGVSTQHTKYVIIAPKDYAVRYLSLNGAPPPVITEDKDKKIYTWEAANLPARLRESHG
                     PRWREIMPDVLIGPSDFEVDGYKGNMTTWLEYGKFVGQLWAGRDVLPDNIRQQVHAMV
                     DTIKDTRKKVYTLYHYLQQNTHYINVSLGIGGWQTFPAQYVATKKYGDCKALSNYMVA
                     LLKEAGIPARYVIIRATENAPPMVEAFPSLNQFNHVIACVPLAKDTIWLECTSQTVSP
                     GYMGTFTGGRKALLIDADGGHVVHTPTYSAKDNTQYRSVKAQIGADGNLDADVSTVYR
                     CVPSDLPHEMMDELTGEQREKYLNELFYLPTYKVEKSQYEEVQGPLPTVKEDLHVVAP
                     NYAGVSGRRLFIAPNVFDRSRTRLPADSVRKYDYITDEAYTDIDSAVIDVPSGYQPEA
                     MPKDVAIEGKFGHYRSSVRFDGDRLVYYRYLQQSAEHFPPTDYAALVKFYEQLYQSDN
                     QRVVLVKKE"
     gene            complement(2977..5001)
                     /locus_tag="VGQ51_12640"
     CDS             complement(2977..5001)
                     /locus_tag="VGQ51_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEV9037471.1"
                     /translation="MNPAQRRLFLAGACCLLLPALHAQDKMPVRFGKVTPDDFKVSAP
                     ATDSAGAVVVADFGTSEFQGNNRGGFDILFRHSRRIRILKRNAFDAATVTIPLYVEGS
                     DMEKIENMKAVTYNLEDGKVVETKLDDKSVFTDKLDKHHIQKKFTFPALKEGSILEYT
                     YTQASPFITELQPWAFQSEYPCLWSEYQVDMPAFFRYVTLSYGFVPFTINTTDERTEK
                     FNLVSPGGADRDQRTTIEDQVVTHRWVMKGVPALKAEPFTTSLYNYLARIEFQLAAVQ
                     FTQFNYYKNYMNDWSKLSEELLAAEDFGADLDNSNGWLEEDCKTVTQGANTSLEKAER
                     IFAFVRDKFNCTSGSGLNLSGPIKTVFRNRNGNVADVNLLLTAMLRHEKIKADPVILS
                     TRSHGFTNEVYPLLDRFNYVITRVRIDSSEYYLDASEPWLGFGRLPERCYNGYARVLN
                     KEQPSFVSLDADGMDEKKMTLVILNKDEKGGLSGRLQTTPGFNEACSIRENIKESGEK
                     EFTKKIETSYTGDESASNFEIDSLKRPDDPLQVAYDLRITPDPGSDIFYFNPMMGEAY
                     KENPFKAAERKYPVEMPFAMDETYTMNMEVPDGYVVDELPKSAKVLFNDDEGFFEYLI
                     VKGTDGIQFRSRIKLNKANFKPEDYETLREFFGYIVKKQSEQIVFKKKKA"
     gene            complement(5033..>5715)
                     /locus_tag="VGQ51_12645"
     CDS             complement(5033..>5715)
                     /locus_tag="VGQ51_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3858 domain-containing protein"
                     /protein_id="HEV9037472.1"
                     /translation="LNKELPSYVSLNADDMNEKKRTLVILNKDDKGGWNGRLTTTPGF
                     NEACAIREKIKGSGEKEYASKIETAYTAGGESVENFEIDSLKRPDDPLQVVYDLRIVP
                     DPGSDIFYFNPMMGEGYKENPFKAAERKYPVEMPYAMDETYSLTMDVPDGYVVDELPK
                     SAKVLFNDTEGFFEYLIAKNDAGIQLRSRIKLNKANFKPEDYATLRDFFGYVVKKQSE
                     QIVFKKKK"
BASE COUNT         1255 a         1637 c         1414 g         1409 t
ORIGIN      
        1 gacgattcag tttgttcatg atccctctgc tattctgtct tacagttgcg gcccaatcca
       61 attttgccgc tgtcgatcaa ttgcttaagc aaaaccaaaa gaccctggcc aacttcgtcg
      121 tcctcgtgtc caaagacggc aagatcgttt accaaaaaca aggaggtacc gatttcaccg
      181 cgaagatgca agccccgatc gccggctccg gcgactggat gacggccgcc ctggtcatga
      241 ccttcgtcga cgaaggcaag ctctcgctgg acgataaggt cacccgatac atccccctct
      301 tcggaaagta tatgaagggc tatatcacca tccgcaattg tctgaccaat accacaggca
      361 tcaagacgga cgaggcggtg gagaaaccca tggaacgaaa tcatttcgaa accctggaag
      421 acatggtcaa cacctatgcc aacaaacatg ccatcgcgac caaccccggc accgagatct
      481 tctatagcaa tctcggaccg aatatcgccg gacgcatact cgagatcgtt tcgaaaaaag
      541 cattcgacag gctgatgcgc gagcggatca ccaacccgct gaagatgcgc gggaccagct
      601 tcaccaacga ggaaggcgga gctaccaatc cttccaacgg cgcccggtcc actgcgaatg
      661 attatatcaa ttttatgacc atgctgctca ataaaggggt attcgagggg aaacgcatcc
      721 tcagcgaaaa ggcggtggcg gagatcgaga cggcgcaatt ccccagcctt ccggtcaaat
      781 tcatgccggc gggtctgcag ggcgcgcata tgggtctggg ctgctttatt accaatacgg
      841 gttcctcggg tgctcccacc gccttcgtca gtcacagcct gctcggagcc gcggcgttca
      901 tggacacctg tcgcaactac agcgcgatca tcatcgtgga aaaaccccag gaagaaaaga
      961 aacccctcta tcagaatctc atgaacctta tcggcgaggc catcgggggc gcctgtaact
     1021 gatctgccgg ggcatcccca tacagcccct caacgatccc ccggcgcata cccgcggatt
     1081 actctttttt caccaatacc acccgctggt tgtccgactg gtacagctgc tcatagaatt
     1141 tgaccagcgc ggcataatcg gtagggggaa aatgttccgc gctctgctgc aaataacgat
     1201 aatagaccag cctgtcccca tcgaaacgaa cgctcgaccg gtaatgaccg aacttcccct
     1261 cgatcgccac atccttcggc atagcctcgg gctgataacc ggacggtaca tcgatgaccg
     1321 cgctgtcgat atccgtatag gcctcatcgg tgatataatc gtatttgcga acgctgtccg
     1381 caggaagcct ggtcctgctc ctgtcgaaga cattcggggc aatgaacagc cgtctgcccg
     1441 atacgccggc ataattgggc gcgacgacat gcaggtcttc cttcacggtc ggcagcggtc
     1501 cctgcacctc ttcatattgg cttttttcca ccttataggt gggcagataa aaaagttcat
     1561 tcaggtattt ttcgcgttgc tcccccgtca gctcatccat catctcatgg ggaagatcgg
     1621 acggaacaca gcggtatacc gtgctgacgt cggcgtcgag attgccgtcg gcgccgatct
     1681 gcgcttttac gctccggtat tgagtattgt ccttagcaga ataggtcggt gtatgaacca
     1741 catggccccc gtccgcgtcg atcagcagcg ccttcctccc accggtgaag gtgcccatat
     1801 agcctggaga taccgtctgg ctcgtacatt ccagccagat cgtgtccttt gccagcggca
     1861 cgcaggcgat cacgtggttg aactggttga gactgggaaa cgcctccacc atgggcggag
     1921 cgttctccgt ggccctgatg atcacatacc gggcagggat acctgcttct ttcaatagcg
     1981 cgaccatata attggacagc gccttgcagt ccccgtattt cttcgtggcc acgtattgcg
     2041 ccggaaaggt ctgccagccg ccgatgccca gcgagacatt gatgtaatgc gtgttctgct
     2101 gcagatagtg ataaagcgta tatacttttt tccgcgtgtc ttttatcgtg tccaccatag
     2161 catgcacctg ctgccgtata ttgtccggca atacatccct gcctgcccat agctgtccga
     2221 cgaatttgcc atattccagc catgtcgtca tattgccctt ataaccgtcc acttcgaagt
     2281 cggaaggacc gatcagcaca tcgggcatta tttcgcgcca ccgcggccca tgcgattccc
     2341 ggagccgcgc aggcagattg gcggcctccc aggtatagat cttcttatcc ttgtcttcgg
     2401 tgatgacagg cggcggcgcg ccattcagcg agagatagcg cacggcataa tccttcggcg
     2461 cgatgatgac atacttcgta tgctgggtgg aaaccccgga ggtcgccagc ggaagccagt
     2521 catcgaaccc aaatatcccg tcatgctcaa tttctttctg atagtcgacg gtataggggt
     2581 acacctggca atagaaatta tgttcgagat accggtcatc ggaagcgaga gatgattcgt
     2641 cgacatagcc cttgtcctgc atatcctttc gtttagcctt gcggaccact ttcccgagac
     2701 tatcgtataa tacgccggat atatcgcgga tggactcgaa tttgtcataa cctaccatga
     2761 tccctccgag attgtcgcca ttcgggttca ggacggtata tacctcatgt tcccgctcga
     2821 ccgccttcgc cggcgatttg atctcaagga tcagctcgtc ctcccggacc acggccctgg
     2881 ctcccttcct cagcgaatcc gggatcagcg ccgtactgta gttctgcgcg gacgccagcc
     2941 cgggcgccag cagcaacaac caataaagga tacttctcat gcttttttct ttttaaatac
     3001 gatctgctca ctttgtttct tgacgatata cccgaaaaat tccctcaggg tttcatagtc
     3061 ctccggcttg aagttcgcct tgttcagctt gatacgcgac cggaactgga tgccgtcggt
     3121 gcctttcacg atcaggtatt cgaaaaaacc ttcatcgtcg ttgaacaata ctttcgccga
     3181 tttgggcagc tcatcgacca catacccatc gggtacttcc atgttcatgg tataggtctc
     3241 gtccatggca aagggcatct ccaccgggta tttccgctcg gccgccttga agggattctc
     3301 tttataagcc tcgcccatca tggggttgaa atagaagatg tccgatccgg gatcgggggt
     3361 gatccgcaga tcataggcca cctggagggg atcgtccggg cgtttcaacg aatcgatctc
     3421 gaaattgctg gccgattcat ctcccgtata ggacgtctct atcttcttcg taaattcctt
     3481 ttcaccggat tctttgatat tctcccggat ggagcaggcc tcgttgaatc ccggcgtagt
     3541 ctgcagacgc ccgctgagac cccccttctc gtccttgttg aggatcacca gcgtcatctt
     3601 cttttcatcc atgccgtctg catccagcga cacaaacgac ggctgttcct tgttgagcac
     3661 ccgcgcatag ccattatagc acctttcggg caaccggcca aaacccagcc agggttcgct
     3721 agcgtcgagg taatattcgg aggagtcgat cctgacccgc gtgatcacat aattgaaccg
     3781 gtccagcagg ggatagactt cattggtaaa accatgcgac ctcgtgctca ggatcaccgg
     3841 atcggctttg atcttctcat gacgcaacat ggcggtcaac agcaggttca cgtcggccac
     3901 gttgccattc ctgttcctga ataccgtctt gatgggaccc gacaaattca gaccgctgcc
     3961 ggaggtgcag ttgaatttgt cccgcacgaa tgcaaagata cgttccgctt tctcgagcga
     4021 agtattcgca ccctgggtca ccgttttaca atcctcttcc agccatccgt tgctattgtc
     4081 gaggtctgcc ccgaaatcct ccgccgccaa tagttcctcg cttagcttag accaatcgtt
     4141 catatagttc ttataataat tgaactgggt gaattgaacg gccgcgagtt ggaactctat
     4201 ccgtgccagg tagttgtaca gcgaagtcgt aaagggctcc gccttcagtg ccggaacacc
     4261 tttcatcacc cagcgatgcg tgaccacctg gtcttcgatg gtcgttcgct ggtccctgtc
     4321 agctcctccg ggagagacga ggttgaactt ctccgtccgt tcatcggtgg tgttgatcgt
     4381 gaacggcaca aagccatagc ttagcgtgac ataccgaaaa aatgcgggca tgtccacctg
     4441 gtattcgctc cacagacagg gatattcgct ctggaacgcc cagggctgaa gttcagtgat
     4501 gaacggcgac gcctgggtat aggtatactc caggatcgaa ccttccttca atgccgggaa
     4561 agtgaacttc ttttgaatat ggtgcttgtc aagcttgtcg gtaaagacag atttgtcgtc
     4621 cagtttcgtc tcgacgacct taccgtcttc caggttatat gtcaccgcct tcatgttttc
     4681 gattttctcc atgtcgctgc cctcgacata taagggtatg gtgaccgttg cagcgtcgaa
     4741 cgcgttccgc ttcagtatcc tgatcctccg cgaatgtctg aacaagatat cgaaaccgcc
     4801 tctgttattg ccctggaact cgctcgtccc gaagtcggca acgacgacgg cgcccgccga
     4861 gtcggtcgca ggcgcggaaa ctttgaagtc atcgggagtc actttcccga atctgacagg
     4921 catcttgtcc tgtgcatgca gcgccggcaa cagcagacaa catgcgccgg ctaaaaataa
     4981 tcttctttgt gcagggttca tggtagttag gtttataagg gttagtaatg aactatttct
     5041 tctttttaaa cacgatctgc tcgctttgct tcttcacgac atacccaaag aagtcccgca
     5101 gcgtggcata atcctccggt ttgaaattgg ccttgttcag cttgatacgc gaccttagct
     5161 ggatgccggc gtcgttcttc gcgatcaggt attcgaaaaa accttcggta tcattgaata
     5221 acactttggc cgatttgggc agttcatcga ccacatatcc gtcgggcacg tccatggtaa
     5281 gtgaataggt ctcatccatt gcgtagggca tttcaacggg gtatttccgc tctgccgctt
     5341 tgaagggatt ttctttatat ccctcaccca tcatcgggtt gaaataaaag atgtccgatc
     5401 cgggatcggg gacgatccgc aggtcgtaaa ccacttgcag gggatcatcg gggcgtttta
     5461 acgaatcgat ctcgaaattt tcgacggatt cgcctccggc cgtataggcc gtctcgatct
     5521 tgctggcata ttccttctcg ccggatcctt tgatcttctc ccgaatggcg catgcctcat
     5581 tgaagcccgg cgtggttgtc agacggccgt tccaaccgcc cttgtcatct ttgttcagga
     5641 tcaccagggt gcgctttttt tcattcatat cgtctgcgtt cagcgagaca taagacggca
     5701 gctccttatt aagca
//
LOCUS       DASYSV010000412        11559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1489821, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000412 DASYSV010000000
VERSION     DASYSV010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489821"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VGQ51_12650"
     CDS             <1..269
                     /locus_tag="VGQ51_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037473.1"
                     /translation="FMFSMLEGWIDKVNQIRTYKIVCPFQNETLHRYEEIFKTHHLKF
                     KRTRQGKTADDLISGEWVVQGSEKNHRHVIHWLLRDPSVRVFEY"
     gene            complement(316..1185)
                     /locus_tag="VGQ51_12655"
     CDS             complement(316..1185)
                     /locus_tag="VGQ51_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEV9037474.1"
                     /translation="MYTIEDDRLQVSIHPKGAELQRIVHKEYRLDYLWGGNPAFWGKH
                     SPLLFPIVGTLKDNTYIYRNRTYHLPRHGFARDREFILEEYSPTAIRFLLRYDAETLA
                     VFPFSFELRVGYRLTYEGLKTTYRVDNPGEMPLYFSIGGHPAFNLPLTPDTVYSDYYL
                     EFDQAEKTPRWPISKDGLIESNPIPLLKDTGRLPLSKDLFAKDALVLKHPASSAVTLR
                     SDNTERGLRMEYPDFPYLGIWAAPGADFVCIEPWCGIADGVGSDQQLENKEGINRLGG
                     GGVFERTWTLRVF"
     gene            1248..2498
                     /locus_tag="VGQ51_12660"
     CDS             1248..2498
                     /locus_tag="VGQ51_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037475.1"
                     /translation="MIRSFRIIWTSLKMALQEFRSNKLRTFLSLLGIIIGIFCIISVL
                     STISSLQQAIDNDLKAFGNKTIYIDKWQYTGGPDYPWWKFVKRPAPKFDELRLLRMKV
                     PGASHFAFEMDTQDNVEAGDNSLSGVTYYGVTEEFDKIQQVIVSDGRYFQQGDFDRGE
                     PLVVMGFRIAERLFGRSQWAVGQTVKLRGGKYATVVGVVEKQGQSIIGGWDFDNAIVV
                     TYNFMRQMIRQENGNPKLLVQAGPNLPIDALTDELKGAMRSIRKLSPTEEDNFALNNI
                     DTFAKQLESVFSGINIGGWFIAGISLLVGMFGVANIMFVTVRERTSQIGLKKAIGAKR
                     GMIMTEFLLESAFLCIIGGMIGLGSVWGLTFVISGIFHFHVFIPIGIVLLTVILCLLI
                     GVLSGIFPAFKAARMNPVVAIRSN"
     gene            2548..3579
                     /gene="tsaD"
                     /locus_tag="VGQ51_12665"
     CDS             2548..3579
                     /gene="tsaD"
                     /locus_tag="VGQ51_12665"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV9037476.1"
                     /translation="MRPTSTGPVTILAIESSCDETSCAISRDGIILSNIIAGQAVHEQ
                     YGGVVPELASRAHIQNIVPVVDASLKKAGCTAADLHAIAFTQSPGLIGSLLVGSEFAK
                     SMALALDLPLLAVHHMQAHVLANLIPVPGRDLPSFPFLCLTVSGGHTQIVLCRSALKM
                     EVIGETLDDAAGEAFDKSAKLLGLPYPGGPLIDKYAREGNAARFSFPEPQIPALNFSF
                     SGLKTAILYFLREETDKNPGFIDANLADICASIQQRIVSILLTKFRKAARETGITDLC
                     IAGGVSANSGLRKAFQELGARECWRTFIPPFEYCTDNAAMIAITGHYKYLSGDFAPMS
                     VSASARAEW"
     gene            3638..4357
                     /locus_tag="VGQ51_12670"
     CDS             3638..4357
                     /locus_tag="VGQ51_12670"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV9037477.1"
                     /translation="MPNSWFKFKQFTVQQDRCAMKVCTDACILGAWFADKTAAWSRVL
                     DIGSGTGLLMLMLAQKHKGEIEGIELDLDAYRQLQENIGSSPWTSMLKAYPGDVRGFS
                     FGEKFDFIISNPPFYEGQLPAADAASNLARHSSGLTLSELLAVIDANLTRDGAFGVVL
                     PFQRSEEFAAMALSAHGFSLREKLLIRQTPGHDFFRSILYFSRLPERTATTSELTIHD
                     DKGGYTEDFVRLMKDYYLYLA"
     gene            complement(4339..5541)
                     /locus_tag="VGQ51_12675"
     CDS             complement(4339..5541)
                     /locus_tag="VGQ51_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV9037478.1"
                     /translation="MIKIGLLREGKVPVDNRVALTPAQCKWIHKNAADVRVVAQSSPD
                     RCYTDKEYRSAGVEVTEDVSGCDILLGIKEVPETNLVADKTYLFFSHTRKQQPYNQRM
                     FHAILEKKITLIDYECLEHDDGQRILGFGFFAGVVGAHNGMLAYGRRTGLYELGRVHR
                     QQSFRELIHTYFGVRLPAVKVAVTGSGRVAHGVLEVMNLMEIVEVEPEEFLGRSFAYP
                     VFTQLKGGVLYEHVDSKTYQREDFHQHPDEYRCKFLPYTRKADILMNGIYWDKGMPKL
                     FGWEDLTDPGFRIRTIADITDDKEGSVPCNLGDATMEDPIYGVDKTTRARTAPYLSGS
                     VDIMAVGNLPNELPRDASRYFGEQLIKHVLEDLVRGGSPVIERATMVRGGRLTEPFSY
                     LSDYARYK"
     gene            5686..5769
                     /locus_tag="VGQ51_12680"
     tRNA            5686..5769
                     /locus_tag="VGQ51_12680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5720..5722,aa:Leu,seq:caa)
     gene            complement(6457..7887)
                     /locus_tag="VGQ51_12685"
     CDS             complement(6457..7887)
                     /locus_tag="VGQ51_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9037479.1"
                     /translation="METFIGRKQELMDFRRLLNTGASEFVAVYGRRRVGKTFLIREAF
                     NNNFDFYITGMANVSLATQLANFHLAISKYAPPGMTISPASNWLTAFHQLSLILENQK
                     KGKKVVFLDELPWLDTAKSEFIQALEYFWNSWASARKDVLLIVCGSAASWMINKLIHS
                     NGGLYNRVTHRVKLEPFTLNECEQYFKSRSEAFNDRYQIVQLYMVMGGIPFYLKQVDT
                     SMSAAQNINNLCFQPGGILYDEFDALYRSLFNKSEHHMAIIEVLSKKARGLTRDELIA
                     SSKTSTGGSTTRILSELEESNFIRKYNSYGKKGKGSLFQLTDFYSLFYLKFIKSKDVL
                     DENHWMAGLNTPKQHAWAGYAFEQVCLAHLPQIKKGLGISGIQSAASSWIGGSGPRGA
                     QIDLVLDRSDRVINLFEMKFSIDTFAIDKKCADDLRKKISVFREVTKTRKAIFLTMIT
                     TFGLKMNTHAMSTVQNELTMEDLFAQ"
     gene            complement(8169..9581)
                     /locus_tag="VGQ51_12690"
     CDS             complement(8169..9581)
                     /locus_tag="VGQ51_12690"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9037480.1"
                     /translation="MKKLLFAGTFLLMAFNAISQTSFSPAVKKFIDYDTSSIAFTHCR
                     LADVKHLKVLEDQTVIIRNGIITAIDDSKKLPAPAGCTVIDLTGKSLLPGFVLVHEHM
                     YYAAYSADFSYLHVKQLPFTFPKLYLACGATMIRTAGSVEPYSDLNLKRDIDQGKIPG
                     PEMDVTAPYVEGKGSIFPQMHELSGPAEAKAFVDFWASQGCTSFKGYMFVNKPTLKAA
                     IDEAHAKGLRVTGHLCAVTYREAAEMGIDQLEHGFAASTDFVPGKKENACPLGAPPIA
                     SADSPKVKDLIRYLVNKKVTLTSTLAVLFNMTTLDTTIRPEVLEAMAPDTRNMFLDVY
                     SKMRSPAGNKAMLEEMRMEKMFSDAGGLLTVGTDPTGNGATLAGYGSQQSIELLTNAG
                     FSPIEAIRIATYNGAKALGLEDRIGSIEIGKAADLVVIDGNIAQDIRNIRKIVWVFKK
                     GIGFNSQKIFSSVKGEVGKY"
     gene            complement(9624..11315)
                     /locus_tag="VGQ51_12695"
     CDS             complement(9624..11315)
                     /locus_tag="VGQ51_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037481.1"
                     /translation="MKWIEKYLWLLFPAFLLASCGLDHSNPSREALDQLHLKKGEIIV
                     CGPADKEFGTVDFDISGSKQVREDFNIAVELLHSFEYDEAEKAFAKIIDNSPGCVMAY
                     WGVAMCNFHPLWEPPGKTDLQKGSKAIEIAQSLKKPSRREAAYIDAVASYYKDWNTTG
                     PHDRCVHFEKAMEQLRSEYPGDREATIFYALALDASADPTDITFSHQRKAGKLLDSMY
                     ASAPDHPGIIHYIIHTYDVPGLAVLALPAARKYAEVAPSSAHALHMPSHIFTRLGLWD
                     ESIKINTRSVEAARCYAKSTGIDGHWDEELHGLDYLVYAYLQKGENAAAEEQLKYIST
                     IRKVYPINFKEAYTFAASPCRLALENKNWAIAAQMPLEPVSFPWDKFPWQEAIVHFGR
                     LLGSVHTGDMPAAQTELSRLTTLRDILVDQKDAYKARQVEIQITAARAWIAHAGGGEI
                     EALNMMKKAADMEDSTSKHPVTPGEVLPARELYADLLFEMHRNTAAFREYEAVLKKCP
                     NRFNSLFGAGRTAELLRDTSNAGVYYKQLLVIADQRSGRSELDVAKAFLKLPVRR"
     gene            11437..>11559
                     /locus_tag="VGQ51_12700"
     CDS             11437..>11559
                     /locus_tag="VGQ51_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV9037482.1"
                     /translation="MRRDIFQAIADPTRRAILVLIAVQAMTPNAIAGHFDITRQS"
BASE COUNT         2712 a         3041 c         2885 g         2921 t
ORIGIN      
        1 tgttcatgtt cagcatgctc gaaggctgga tcgataaggt caaccagatc aggacctaca
       61 agatcgtgtg tcctttccag aacgagaccc tccaccgtta tgaagagatc ttcaaaaccc
      121 atcatcttaa attcaaacgc acccgccagg gcaagacggc ggatgacctc atatcgggag
      181 aatgggtcgt gcagggctcc gaaaagaacc accggcatgt tatccattgg ctgttacggg
      241 acccctcggt cagggttttt gaatattgag cgccgtctcg tcgctctccc gggtacgacg
      301 gagccgctga acaggctaaa acacccgcag cgtccaggtc cgttcgaaca cccctccgcc
      361 tccgagccgg ttgatccctt ctttgttctc cagctgttga tcgctgccta ccccatcggc
      421 aatgccgcac cagggttcga tgcagacaaa atcggccccc ggcgccgccc agatccccag
      481 gtatgggaaa tcgggatatt ccatcctcaa ccccctttcg gtgttatccg accgcaacgt
      541 caccgcggag gaggccgggt gcttcagcac caatgcgtct ttggcaaaaa ggtccttgct
      601 aagcggaagc ctaccggtgt ccttcagcaa gggtatggga ttcgactcga tgagcccgtc
      661 tttggatatt ggccatcggg gtgtcttttc cgcctggtcg aactccaggt aatagtcgga
      721 ataaaccgta tccggcgtca gcggaaggtt gaaagcgggg tgaccaccga tcgaaaaata
      781 tagtggcatc tcgccgggat tatcgacacg gtaggttgtt ttcaacccct cgtaggtgag
      841 acggtagccg acccgaagct cgaaagagaa cgggaaaacg gcgagggttt cggcatcgta
      901 gcgcaacagg aaacggatgg cagtcgggga atactcttcc aaaatgaact cgcggtccct
      961 ggcaaaaccg tgccggggaa ggtgataggt cctgttccgg tagatataag tattatcttt
     1021 tagcgtaccg acgatgggga acagcagggg tgaatgtttg ccccagaatg ccggattgcc
     1081 gccccacaga taatccagcc ggtattcttt atgaacaatc cgctgtaatt ccgcgccttt
     1141 tggatgaatg ctgacctgga gccgatcgtc ttctatcgtg tacatgtctt tttttccgtt
     1201 tatcacgcaa tttactccat ttgaacgcga atctgtattt tcgggatatg attcgctcct
     1261 tcaggatcat ctggacaagt ctaaaaatgg cgttgcagga gtttcgctcc aacaagctgc
     1321 gcaccttcct gtccctgctg ggcatcatca tcggcatttt ttgtatcatc agtgtccttt
     1381 cgaccatcag cagtctgcaa caggcgatcg acaatgacct gaaggcattc gggaataaaa
     1441 cgatctatat cgacaaatgg caatacacgg gtggaccgga ttatccctgg tggaaattcg
     1501 tgaagcggcc cgcgcccaaa ttcgatgagc tgcgattgct ccggatgaag gttccgggtg
     1561 cctcgcattt cgccttcgaa atggatacgc aggataatgt ggaggccggc gacaatagcc
     1621 tttcgggcgt cacctattat ggggtcaccg aggaattcga caagatacag caggtcatcg
     1681 tcagcgacgg ccgctatttc cagcaaggcg atttcgacag gggcgaaccc ctggtcgtga
     1741 tgggcttccg aatcgccgaa agattgttcg gacgatcgca atgggcggtg ggacagacgg
     1801 tgaagctccg cggcgggaag tatgcgaccg tcgtgggcgt ggtggagaaa caagggcaga
     1861 gcatcatcgg cggatgggat ttcgacaatg ccattgtagt cacgtataat tttatgcggc
     1921 agatgatccg gcaggagaac ggcaatccca agctgctcgt gcaggccggt cccaacctgc
     1981 ctatcgatgc gctgacggac gagctcaaag gagccatgcg atcgatccgg aagctgagtc
     2041 ctaccgaaga ggataatttc gcgcttaata acatcgatac ttttgcgaaa cagcttgaat
     2101 ccgtttttag cggtatcaac atcggcggct ggttcattgc cgggatatcc ctgttggtcg
     2161 ggatgttcgg cgtcgccaat atcatgttcg ttacggtgcg cgaacggacc agccagatcg
     2221 gtctcaaaaa agccatcggg gcgaagcgcg gcatgatcat gaccgaattc ctgctcgaat
     2281 cggcctttct ttgtatcatc ggcgggatga tcgggctcgg aagcgtatgg ggattgacgt
     2341 ttgtcatctc gggcatcttt catttccatg tgttcattcc catcggcatc gtgcttctga
     2401 cggttatcct ttgcttgctc atcggcgtgc tgtccggtat cttccccgcc ttcaaagcgg
     2461 cccggatgaa cccggtggtg gcgatccggt cgaactgagc atgcggggac tactccacgc
     2521 cccgccactt tttcctacct tcgcgccatg cgaccaacga gtaccggacc cgtgaccata
     2581 ttagccatcg aatcttcctg cgacgagacc agttgtgcga tcagccgtga cggaatcatc
     2641 ctctccaata tcatagcggg acaggcggtt cacgaacaat atggaggcgt cgtccccgag
     2701 cttgcgagca gggctcatat ccagaacatc gttcccgtcg tggacgcatc cctcaaaaaa
     2761 gccggctgca cggccgccga tctgcatgcc atcgctttta cacaatcccc cggcctcatc
     2821 ggatcgttgc tggtcggctc ggagttcgcc aaatccatgg cgctggcgct ggaccttcct
     2881 ttgctggcgg tgcaccatat gcaggcgcat gtgctggcca atctcatacc ggtacccgga
     2941 cgggacctac cttccttccc ctttctttgt ttgaccgtca gcggcggaca cacgcagatc
     3001 gtactgtgtc gttcagcgct gaaaatggag gtcatcggcg agaccctcga tgatgccgct
     3061 ggcgaagcct tcgacaaatc ggccaaactc ctgggccttc cctaccccgg cggaccgctt
     3121 atcgataaat atgcacggga aggcaatgcc gcgcgttttt cctttcccga accccagata
     3181 ccggcgctga atttcagttt cagcggtctc aaaacggcca ttctttattt tctccgcgag
     3241 gagactgata agaaccccgg atttatcgac gccaacctgg ccgatatctg cgcatccatc
     3301 cagcaacgca tcgtctcgat cctgctgacc aaattcagga aggcggcccg cgagacgggc
     3361 atcaccgatc tttgcatcgc cggcggcgtc tccgccaata gcgggctgcg caaggcgttc
     3421 caggaattgg gcgcgcgcga atgctggcgg acattcatcc cacccttcga atattgcacc
     3481 gataacgcgg ccatgatcgc catcaccggg cattacaaat atctttccgg cgatttcgcg
     3541 ccaatgtctg taagcgccag cgcgcgggcg gaatggtagg cgccgttcgc gtgggaaatc
     3601 ctggccgggt cgcgcgcgat gttctaaatt tgccgcgatg cccaactcct ggttcaaatt
     3661 caaacaattc acggtccagc aggaccgctg cgccatgaag gtctgcacgg atgcctgtat
     3721 cctcggcgcc tggttcgcgg acaaaactgc ggcatggtcc cgggtgctgg atatcggcag
     3781 cggcacgggt ctgctgatgc tcatgcttgc acaaaaacac aagggtgaga tcgaaggcat
     3841 agagctcgat ctcgacgcct accgccaatt gcaggagaat atcggcagta gtccctggac
     3901 ctccatgctc aaggcctacc cgggcgacgt ccgcggtttt tctttcgggg aaaaattcga
     3961 tttcatcatc tccaacccgc cgttctacga aggacagctg cccgccgccg acgccgcgtc
     4021 caatcttgcc agacacagta gcgggctcac cctgtccgag ctgcttgccg tcatcgatgc
     4081 aaacctcacc cgggatggcg cgttcggggt cgtcctccct ttccaacgct cggaagagtt
     4141 cgcggcaatg gcgttgtccg cgcatgggtt ctcgcttcgt gaaaaattgc tgatccgtca
     4201 gacgcccggt catgattttt tcaggagcat cctgtatttc tcgcgtcttc ccgaacgaac
     4261 ggccacgacg tcggaactga cgatccacga tgacaagggc ggctataccg aagatttcgt
     4321 tcggctgatg aaggattatt atttgtatct cgcataatct gacaggtagc tgaagggttc
     4381 cgtcagccgc ccgccacgca ccatcgtcgc ccgttcgatg acaggcgatc ctccgcgcac
     4441 caggtcttcc agcacatgtt tgatcagctg ttccccgaaa taacgggatg cgtcccgtgg
     4501 caattcattg ggaagattgc cgacggccat gatgtctacc gagcccgaaa gataaggcgc
     4561 cgttcgcgcc cgcgttgttt tgtccacacc gtagatgggg tcttccatgg tcgcatcgcc
     4621 caggttgcag ggaaccgatc cttccttgtc atcggtgatg tcggcgatcg tccggatccg
     4681 gaaaccggga tcggtcagat cctcccatcc gaacagtttt ggcatcccct tgtcccaata
     4741 gatgccattc atcaggatgt cggcttttcg cgtataaggg aggaatttgc agcggtattc
     4801 atcgggatgc tgatgaaaat cttcgcgctg ataggtcttg ctgtcaacgt gctcatataa
     4861 gacgccgcct ttcagttgcg taaaaacggg ataagcaaaa cttcgcccga ggaattcctc
     4921 cggctcgacc tccacgatct ccatgagatt catgacctct agcactccat gcgccaccct
     4981 gcccgacccc gtgaccgcta cttttacggc agggagacgg acgccgaaat aagtatggat
     5041 caattcgcga aaactctgct gccggtgaac ccgccccagc tcataaagcc ccgttcgcct
     5101 cccataggcc aacatcccgt tgtgcgcgcc tacgacaccc gcaaaaaaac cgaaacccaa
     5161 tatccgctga ccgtcgtcgt gttcaagaca ttcgtaatcg atgagggtga tctttttttc
     5221 caggatcgca tggaacatcc tttgattgta cggttgctgt ttgcgggtat gcgagaagaa
     5281 aagataggtc ttgtccgcaa cgagattcgt ttccggcact tctttaatac ccagcagaat
     5341 atcacagccg gagacatcct ccgtgacctc cacccccgcg gagcgatatt ctttgtcggt
     5401 ataacagcga tccggcgaag attgcgccac cacccgcaca tccgcagcgt tcttatgtat
     5461 ccatttgcat tgggccggcg tcagcgccac cctgttgtcc acgggtactt ttccttcgcg
     5521 taataagcca attttgatca tgctatcctc gaagatagaa ttttttgccc ctttttggac
     5581 aacacccgcc aaatccgtaa tattgcgtcc cgcttttccg gtgccggcgc tccgcgccac
     5641 atccaacgga acccgtcgcg gttcgccccg cggcgcgcgc ctcatgccca gatggcggaa
     5701 ctggtagacg cgtcagactc aaaatctggt attggcaaca atgtgcaggt tcgattcctg
     5761 ttctgggcac tcaggttaac aaccgattaa gaaggtcgcg aatttcgtga ccttttttat
     5821 ttgcaaacaa tttgcaaaca cgattttcgg cggaggtaag aaagagcttt caattgcaaa
     5881 tgtaatgtaa ttacgagccc ggaacgtata ctgcgatctc aaatcttaac ttagcagtct
     5941 gaccctttgg tcccagaagg gggagaattt agctctcata atggaattaa cttataacaa
     6001 agatggtttt gtgttgtatc tgctgcagaa tgatgttgat acatcgccaa taaatattct
     6061 tactaacctg aaggtattaa ggatagacga attaagaagt actttggatt tttcgaactt
     6121 caaaaatctg agagttctta gtctaacgca taataaaaac gttcaaaatt taggttcttg
     6181 taagacgtta ttttggcttt ggattgaaaa atataaaaag gagaatctta ttgaattaag
     6241 agacttagtt aatttagagt ttcttaccct tcacggaggt tcagtgcata ctttgaaggg
     6301 aatggagtcc atgactaacc taaaaatgtt gcgcatggat accttaaaga acctcgagtc
     6361 gttgcaggga ttaaattcca tgatgaacca acttgagatt ttagatatat atatgtccag
     6421 gacgttgacg gactatactt gtatagccga aagggcttat tgtgcaaaca aatcttccat
     6481 tgttagttca ttctggaccg tagacatggc gtgcgtgttc attttcaggc cgaatgtcgt
     6541 aatcatggtt aggaatattg ccttcctggt tttagtcact tcgcgaaaaa cggatatttt
     6601 ctttctgaga tcgtccgcgc attttttgtc aatcgcaaag gtgtcaatgg aaaatttcat
     6661 ttcaaacaag ttgataactc tatcactgcg gtcgagaacc aagtcaatct gtgcgccgcg
     6721 tggacccgaa cccccgatcc aggaagaggc ggctgattgt atgccagaaa tgccaagtcc
     6781 cttctttatc tggggcagat gggctaggca tacctgttca aaagcatatc cggcccaggc
     6841 atgttgtttt ggtgtattta aacctgccat ccaatgattt tcatccagca catcttttga
     6901 ttttataaac ttcagataaa acaaggagta aaaatcagta agctggaaca aactgccttt
     6961 gccttttttg ccataggagt tatattttct gatgaaattg ctctcctcca actcactcag
     7021 tattctggta gtagagccac cggtcgaggt tttcgaagaa gcaatcaatt cgtcccgggt
     7081 cagtcccctg gcttttttac tgagcacttc tatgatggcc atatgatgct ccgatttgtt
     7141 gaataatgac ctgtaaaggg catcaaattc atcatacaag atcccgccgg gttgaaaaca
     7201 taaattgttg atattttgcg cagcgctcat agaggtgtct acctgtttca ggtaaaaggg
     7261 gatgcctccc attaccatat agagctggac tatttgatac ctatcattaa aggcctccga
     7321 ccggctttta aaatattgtt cgcattcatt caaagtaaaa ggttccagct tcactcgatg
     7381 agttaccctg ttatatagcc ccccattgct gtgtatcagt ttgttaatca tccacgaagc
     7441 tgctgaacca cagacaatca atagcacatc ctttcttgca ctggcccatg aattccagaa
     7501 atattccagg gcttgtataa attccgattt cgccgtatca agccatggca actcatccag
     7561 aaacacaact tttttccctt ttttttgatt ttccaaaatc agcgaaagtt gatggaatgc
     7621 ggtcagccag ttagaggccg gactaatagt catcccgggt ggggcatatt ttgagatagc
     7681 tagatgaaag tttgcaagtt gggtagctaa cgaaacattg gccatcccgg tgatgtagaa
     7741 gtcaaaattg ttgttaaagg cttctcttat cagaaacgtt tttccgaccc gcctgcgtcc
     7801 gtacacggca acgaattccg acgcgcccgt attcaacaag cggcgaaaat ccatcaattc
     7861 ctgttttcga ccgatgaagg tttccatatt ctaatttggc caaatatacc acaaatgcat
     7921 agcttttggc caaatatact gatataatta gccaaatatt gtaacctaaa gaaacttttg
     7981 gccaaatatg tcccagatcg tagatacatc ggaaactgaa catcgggttt tggtgaccct
     8041 tgaatcaggt attccaaaat tagcagtacc ggtcgaattg ctacctgcct ggtttcgcac
     8101 ttacttcccg gcgatcgtcc agcggataag cgaccatgaa ccgaaattcg gttaagttgc
     8161 aactatcttc agtatttccc aacctcccct ttcacggaac tgaatatctt ctgggaattg
     8221 aaccctatcc ctttcttgaa cacccacacg atcttcctga tattgcggat atcctgcgca
     8281 atattaccat cgatgaccac gagatcggcg gccttaccta tttcgatgga gccgatcctg
     8341 tcttccagtc cgagggcctt tgcgccgttg tatgtggcga tccggatggc ctctatgggg
     8401 ctgaaccccg cattcgtcaa caattcgatc gactgctgtg agccgtaccc cgccagagtg
     8461 gcgccgttgc cggtaggatc ggtacccacg gtcaacagac cgccggcatc tgaaaacatc
     8521 ttttccatcc tcatttcctc cagcatggct ttgttgccgg cggggctgcg cattttgctg
     8581 tagacatcca ggaacatatt tcgggtatcg ggcgccatcg cctccagtac ttccggacgg
     8641 atggtagtat ccagggtggt catgttgaag agtacagcga gggtggatgt cagggtcact
     8701 tttttattga cgaggtatcg gatcaggtct ttgactttcg gcgaatccgc ggatgcgatg
     8761 ggtggcgcac caagcggaca ggcattctct tttttaccgg gaacaaaatc cgtggaggca
     8821 gcaaagccat gttccaactg gtcgataccc atttcggccg cctcgcggta ggtaacggca
     8881 cataaatgcc ccgtcactct tagtcctttt gcatgggctt cgtctattgc ggccttcagg
     8941 gtaggcttat tcacgaacat atatcccttg aatgaagtgc agccctggct ggcccagaaa
     9001 tcgacaaagg cttttgcctc cgcgggtccg ctcaattcat gcatctgggg gaagatgctg
     9061 cctttccctt ccacgtacgg cgcggtgaca tccatttccg gtccggggat cttgccctga
     9121 tcgatgtccc gtttgaggtt cagatcggaa tagggctcaa cgcttccggc cgtacgaatc
     9181 atcgttgcgc cgcaagccag gtatagtttt ggaaaagtga atggcaattg tttgacatgc
     9241 agatagctga agtcggcgga gtacgccgca tagtacatgt gttcgtgcac taggacgaaa
     9301 ccgggtaata atgatttgcc cgtgagatcg ataacggtgc aaccggcggg ggcgggaagt
     9361 tttttgctat cgtctatggc agttattatt ccgttgcgga tgatcacggt ctggtcttcc
     9421 agcaccttca gatgtttgac atcggcgagt ctgcaatgag tgaaggcgat ggaggatgta
     9481 tcgtaatcga tgaatttttt tactgcgggg gaaaacgagg tttgtgagat ggcattgaaa
     9541 gccatgagca ggaaagttcc ggcaaagaga agttttttca tgcggcagcg agttttgatt
     9601 atataatgtt ttttcggtcc atcctacctg cgaacgggca acttcaaaaa ggcttttgcc
     9661 acatccaatt ccgacctgcc cgatcgctga tcggctatta caagcaattg cttatagtag
     9721 acacctgcat ttgacgtgtc ccgcagtagt tcagcggtgc ggccggcgcc aaaaaggctg
     9781 ttaaagcgat tggggcattt tttcaatacc gcctcatatt cccggaatgc tgccgtgttt
     9841 cggtgcattt cgaataaaag gtccgcatat aattccctcg cgggtaagac ttccccggga
     9901 gtgaccgggt gtttggatgt actgtcttcc atatctgcgg ctttcttcat catgttcaac
     9961 gcttcaattt ctcctccccc ggcatgggct atccatgccc tggccgctgt tatttgtatc
    10021 tcgacctgcc tggctttgta ggcatctttt tgatctacca gaatgtcgcg caaggtggtc
    10081 aacctggaca actccgtctg cgcggcaggc atatcgccgg tatggaccga gcccaacagt
    10141 cttccaaaat gaacaatagc ctcttgccac ggaaatttgt cccagggaaa tgataccggt
    10201 tcgaggggca tctgcgctgc tatcgcccaa ttcttgtttt caagcgccaa ccgacagggg
    10261 gacgcggcga aggtataggc ttccttgaaa ttaatgggat acaccttcct gatcgtcgaa
    10321 atgtatttca attgttcctc tgccgccgca ttttctccct tttgcaggta agcatacacg
    10381 agatagtcca atccatgcaa ttcttcatcc caatgcccat ctataccagt cgatttggcg
    10441 tagcatcgcg cggcttcgac tgaccgggtg ttgattttga tgctttcatc ccaaagaccc
    10501 aggcgggtga agatatgcga gggcatatgc aatgcatgtg cggaagaggg ggctacttct
    10561 gcatatttgc gcgcagccgg cagggcaaga acagccagac cgggaacatc gtaagtatgg
    10621 ataatataat ggatgatgcc cggatgatcg ggggcggatg catacatgct gtccagcaat
    10681 ttgcccgctt ttctttgatg ggaaaaggtt atgtctgtcg gatcggcgct ggcatccagg
    10741 gccaatgcat aaaagatggt cgcctcgcgg tcgcccgggt actcacttcg caactgctcc
    10801 attgcttttt caaaatgcac gcaacgatca tggggacccg tcgtattcca gtctttataa
    10861 taggaagcaa ccgcatctat gtatgcggct tcgcgccggg atggcttttt cagggattgg
    10921 gcgatctcga tggctttgga gcccttctga agatccgttt tacccggagg ctcccagaga
    10981 ggatggaaat tgcacatagc cacaccccaa taagccataa cacaacccgg cgaattgtcg
    11041 ataatttttg caaaggcctt ttcagcctca tcatattcaa atgagtgtaa tagctcgaca
    11101 gcaatattga aatcttctct aacctgtttg cttccggaga tatcgaaatc gacggtacca
    11161 aattctttat cggctgggcc gcaaacaatg atctcacctt tttttaagtg cagttgatcg
    11221 agcgcttccc tggagggatt tgaatggtcc aggccgcatg atgccagcaa aaatgccgga
    11281 aagagtaacc ataaatattt ctctatccac ttcatgtctt cacattataa aaagcattga
    11341 tataaattta acaaaaaaac ccagtgcaaa aaaaaatttg cgaaaccgat cggctgcgca
    11401 tatatttgca accgaacggc ttcttatttg atctttatga gacgagacat tttccaggcc
    11461 attgcagacc ccacccgacg agcgatcctc gtcctgatcg ccgtacaggc aatgaccccc
    11521 aacgccatcg ccgggcactt cgacatcacc cgccagtcc
//
LOCUS       DASYSV010000413         7121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1235180, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000413 DASYSV010000000
VERSION     DASYSV010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235180"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VGQ51_12705"
     CDS             <1..694
                     /locus_tag="VGQ51_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9037483.1"
                     /translation="SFDVSEGEIFGLLGPNGAGKSTTLEIIETLREKTAGKIIVDGFD
                     LDKDPGEIKKIIGVQLQTSGYYPGLNLTELIELFGGLYNQPVSPLQLLDTVNLREKAR
                     AKFKELSGGQKQRFSIATTLINKPRIIFLDEPTTGLDPQARRNLWELIKDIRAKGTTV
                     IITTHYMDEAEILCDRVAIIDSGKIIAMDSPDRLIDKLVASGFERPKEVKQANLEDVF
                     INLTGHALRPEN"
     gene            713..1255
                     /locus_tag="VGQ51_12710"
     CDS             713..1255
                     /locus_tag="VGQ51_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HEV9037484.1"
                     /translation="MSTVATEDLLRYPIGRYETRPFSDQQKEAWLNDIKFLPQLLENA
                     IGNLDESQLQTPYRDGGWTVQQVVHHVADSHMNAYCRFKLGLTEDNVTIRPYEEKAWA
                     VLDDVEAVPINVSITLLHALHTRWHAAIADLEPDQWEKVLIHPEYKKEMTLWYLLGMY
                     AWHGRHHVAHITSLRERNKW"
     gene            1265..1822
                     /locus_tag="VGQ51_12715"
     CDS             1265..1822
                     /locus_tag="VGQ51_12715"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV9037485.1"
                     /translation="MKDLSWKTLSSEYLFKETWFTLRKDVCQTRDGKLIDRYYVYEFP
                     TWVIAVALTSDGRFILERQYRHALGMTDYELPGGCVDPSDLSLEDAVRRELREETGYT
                     FGSVEYLGKTSANPSTNNNWAHFYLATGGERTQEQDLDHSEEIEIYLYTVDELRQLLS
                     DNGIVQSMHVTALFYALQKLGYMKF"
     gene            complement(1819..3105)
                     /locus_tag="VGQ51_12720"
     CDS             complement(1819..3105)
                     /locus_tag="VGQ51_12720"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEV9037486.1"
                     /translation="MDYAQTVDYLLTKLPMYSRIGAAAYRKDLTNTLQLSEYIGNPEK
                     KFKSIHIAGTNGKGSTSHMLAAILQQAGYRTGLYTSPHLKDFRERIRINGEFIEEPFV
                     VDFMQRIGPLSEKLEPSFFEISVVMAFEYFAQQKVDIAVIEVGLGGRLDSTNIITPEL
                     SVITNIGYDHMNLLGNTLAAIAFEKAGIIKPGVPVVIGEYHPETAPIFEQRAHEEQAP
                     IRWADKQHYISDWRYQRHSLVAEVSHSPLADDKQYYTLDLPGVYQTKNLLTVLEAVRQ
                     LTAMGWQIEHRHVDRALRQVKKLTGLHGRWEIVHEHPDIVLDVAHNVDGIRQLVRQIE
                     LTDHEQLHIILGMVRDKDIDPVLALLPRDANYFFTRAQIPRALSETELAAKAGATGLQ
                     GHSYPSVARALDAARTHARPKDLILICGSVFVVAEV"
     gene            complement(3147..4028)
                     /locus_tag="VGQ51_12725"
     CDS             complement(3147..4028)
                     /locus_tag="VGQ51_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037487.1"
                     /translation="MSEQFESRKNMKATGYTTVVCAALLILLIGLSWTRPAEPQPVVE
                     EGIEVNLGNSNQGLGKDQPYLPGDPSPQDKEKYTPPKQAVVEKEPVKDVETDDNQKED
                     APVVKKAVVTKPDATKIPDKEVTRVKVKPVKVPETLPEKPKPIPHPKAVFRGVNGTGN
                     GGNNADDYKRGGNEGIAGGHGDQGAPGGDPNSTNYTGGGHGNGAVAIRSGLAGRHIVR
                     TPSFTGDFNENAKVAVTVHVDAGGNVTSAEYSLMGSTTSMANYKDLATEKARQIKFNP
                     GSDESVGTLIFNFRIHN"
     gene            complement(4120..4323)
                     /locus_tag="VGQ51_12730"
     CDS             complement(4120..4323)
                     /locus_tag="VGQ51_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037488.1"
                     /translation="MKRSQAIALAALTTTLAAGVTLFFISKRNRQKRKDFVANAGYEM
                     AYDINYPLRYRRHKRHNGHRSAF"
     gene            complement(4423..5136)
                     /locus_tag="VGQ51_12735"
     CDS             complement(4423..5136)
                     /locus_tag="VGQ51_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV9037489.1"
                     /translation="MQKIIIVGATSGIGRELARLYSAAGHLVGATGRRQELLYSLQLE
                     YPNNIVTECYDATGPGATTHLSSLVRKMGGLHLLIFNAGWGDLTDPLDWQVDKTTVDI
                     NVNAFLETIHFGWQYFLEQGQGHLVTVSSIAANRGNRHSPAYSAAKAFQSTYFEGLYI
                     KTRKLAIPIHITDIQPGFVDTKMAKGPRFWVSPVDKAARQIVRAIEKKRWRVYITHRW
                     WIIAKLFRYIPSWLYHRLG"
     gene            complement(5136..6431)
                     /locus_tag="VGQ51_12740"
     CDS             complement(5136..6431)
                     /locus_tag="VGQ51_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV9037490.1"
                     /translation="MLKESPYILYSDGKGNIFEDTTLHSTGRSGWDALPIDPQDWIAL
                     PDGGSLYELPGRRGIGIDVRTGEMRLCEKGWAVAAFIPPAHTGLFLAAYETTPGAPTL
                     PLFCYTAAGWQDGKFFVPALRIEQDIRQDCAGYDWNVINNGVDRLRSAYPHNRLVRHL
                     ADNCCLTYHCPAARNFFMGRWECPVPSSPACNANCIGCISFQPQEETIPSTQDRLTFK
                     PTAEEIVEFTVPHLQEAPYPIVSFGQGCEGEPLLMWETIREAILGMRKHTDRGSININ
                     TNGSKPAAVQALCEAGLDSIRVSTNSARAPIYEGYYRPNNYTFDDIVESLRVVRNYGG
                     WTSINYFVFPGMTDSIAEYEALRKLIRTTGLNMIQWRNFNIDPDWYLGRLGVTDTGEC
                     MGVRQLMQLIKEEFPGLRYGYFNPPIERIKGKYELDFAH"
     gene            complement(6545..>7121)
                     /locus_tag="VGQ51_12745"
     CDS             complement(6545..>7121)
                     /locus_tag="VGQ51_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037491.1"
                     /translation="APKCQRLKTNGAGLHLLAGALILAHAISHFRGESRHTLYFWCQL
                     LISIDIFLLVLVGRDILKRTPRVNLFFRTVEIIFFLGIGLLMLVEHHAMTGIFHLLLT
                     AGYCYLFYYERSLRSEETLSIHHTGITIPDIPESRFLYWTHINDLTATYDSIDICTSD
                     DKDLRFEFLRNLQFEELERIQAFCQFYLGKP"
BASE COUNT         1521 a         1746 c         2197 g         1657 t
ORIGIN      
        1 cagcttcgac gtcagcgaag gcgaaatatt cggcttactg ggtcccaacg gcgcgggcaa
       61 atccaccacg ctggagatca tcgaaacctt gcgtgaaaaa actgccggaa agatcatcgt
      121 ggatggtttc gacctggaca aggatcccgg tgagatcaag aagatcatcg gtgtccagct
      181 gcagacctcg ggatattatc ccggtctcaa cctgaccgag ctgatcgagt tgttcggcgg
      241 attgtataac cagccggtga gcccgttaca gctgctggat acggtgaatc tccgggaaaa
      301 ggccagggcc aagttcaagg agctcagcgg cggtcagaaa caacggttct ccatcgccac
      361 gacactgatc aacaaaccca ggatcatttt cctggatgag cctactacag gtctcgatcc
      421 gcaggcacgg cgcaacctat gggaattgat caaggatatc cgcgccaagg ggaccaccgt
      481 catcatcacg acgcattata tggatgaggc ggagatcctg tgcgatcgtg tcgcgatcat
      541 cgactcgggg aagatcatcg ccatggattc tcccgacagg ctcatcgata agctggtagc
      601 cagcggtttc gagcgcccga aggaggtaaa gcaggccaac ctggaagatg tgttcatcaa
      661 tttaaccggt catgccctga ggccggagaa ttaaaaaata taatcaacca ttatgagtac
      721 cgttgcaacc gaagatcttt tgcggtatcc gataggccgc tatgagacca ggccgttttc
      781 cgatcagcag aaggaagcct ggctgaatga catcaaattc ctgccacagc tgttggagaa
      841 tgccatcggg aatctcgacg agtcgcagct gcaaacgcct taccgcgatg gcggatggac
      901 ggtacaacag gtggtgcatc atgtggctga cagtcatatg aacgcctatt gccgatttaa
      961 gctcgggctg acagaggata acgtcacgat ccgcccttat gaggaaaagg cgtgggccgt
     1021 gctcgacgat gttgaggcgg tacccatcaa cgtctccatt accttattac atgcattgca
     1081 tacgcgctgg catgcagcga tagccgatct ggagccggac cagtgggaaa aggtgctcat
     1141 tcatccggaa tacaaaaagg aaatgaccct gtggtacctg ttgggcatgt atgcctggca
     1201 tggccggcat catgtcgcgc atatcacgtc actgagggaa agaaacaaat ggtagggcag
     1261 taacatgaag gacctcagct ggaagacgct ttcttccgaa tatcttttta aggagacctg
     1321 gttcaccctg cgaaaggacg tatgccagac ccgggacggg aagctgatcg acaggtatta
     1381 tgtctatgag ttcccgacat gggtgatcgc ggtggcgctg accagcgatg gccggttcat
     1441 ccttgagcgg caataccgcc atgcgctggg aatgaccgat tatgagctgc ccggcggttg
     1501 tgtggatcct tcggatctgt cattggaaga cgccgtccgg cgtgagttgc gcgaggagac
     1561 gggatatacc ttcggctctg tcgaatatct tggcaaaact tccgccaacc cctccaccaa
     1621 taataactgg gcgcacttct acctggccac gggaggcgag aggacacagg agcaggattt
     1681 ggatcacagt gaggagatcg agatttacct gtataccgtg gatgagttgc ggcagctgct
     1741 ttctgacaac ggcatcgtgc agagcatgca tgtgaccgcc ttgttctatg ccttgcagaa
     1801 gctggggtat atgaaatttt agacttcagc caccacgaat acgctgccgc agataagaat
     1861 gaggtctttg gggcgggcat gggttctggc tgcgtcgagt gccctggcca cggaagggta
     1921 agaatgacct tgcaagccgg tcgccccggc ttttgcggcc agctcggttt ctgacagggc
     1981 gcgtggtatc tgtgcgcggg taaaaaaata gttcgcatcg cgcggcagca gggcgagcac
     2041 cgggtctata tccttgtcgc gaaccattcc cagtatgata tgcagctgtt cgtggtccgt
     2101 tagctctatc tgccgcacga gctggcggat gccgtcgaca ttgtgagcca cgtcgaggac
     2161 gatgtcggga tgttcatgaa cgatctccca acggccatgc aggcccgtta gctttttcac
     2221 ctgccggagg gcgcggtcga cgtgccggtg ttctatctgc catcccatgg ccgtcagttg
     2281 gcggacggct tcgaggacgg tgaggagatt tttcgtttga tagacgccgg ggaggtcgag
     2341 ggtataatat tgtttgtcat cggccagcgg ggaatgggac acttccgcaa cgagcgaatg
     2401 gcgttggtaa cgccaatctg aaatatagtg ttgtttgtcg gcccaacgga tgggcgcctg
     2461 ttcctcatgg gcgcgctgtt cgaaaatggg ggccgtctcg ggatgatatt caccaatgac
     2521 caccggcacg ccgggtttga tgataccggc tttttcgaat gcgatcgccg cgagggtatt
     2581 cccgagcaga ttcatatggt cgtagccaat gttggtgatc acggacaatt ctggggtaat
     2641 gatattggtg ctatccagtc ttccgccgag gcccacctct ataacggcga tgtccacctt
     2701 ctgctgtgcg aaatattcaa aggccattac gacggagatc tcgaaaaagg aaggttccag
     2761 cttttcggac agggggccga tgcgttgcat gaaatcgacc acgaagggtt cttctatgaa
     2821 ttcgccgttg atacggatgc gttcgcggaa atctttcaga tggggcgagg tatagagtcc
     2881 cgttctataa ccggcctgtt gtaagatggc ggcgagcata tggctcgtgg agcctttgcc
     2941 gttggtgccg gcgatgtgga tggatttgaa ctttttttca gggttgccga tgtattcgct
     3001 gagttgaagg gtattcgtca ggtcttttcg gtaggcggcg gccccgatgc ggctgtacat
     3061 ggggagtttc gtgaggaggt agtccaccgt ttgagcataa tccatagggg agcaaagata
     3121 cggcggatgg ccgtgcgtcg cggcgcctag ttatgaatcc tgaaattgaa gatcagggtg
     3181 ccgaccgact cgtcgctccc gggattgaat tttatctgtc tggctttttc ggtggcgagg
     3241 tctttatagt ttgccatcga agtggtggaa cccatcagcg agtattcggc gctggtgacg
     3301 ttgccgcccg cgtctacgtg gaccgtcacg gcaaccttgg cattttcgtt gaaatcgccg
     3361 gtaaaggatg gtgttctcac gatatgccgt cccgcaagac cgctgcgaat agcgaccgcg
     3421 ccattgccat gtccgccgcc ggtgtagttc gtggaattcg gatcgcctcc gggggcgccc
     3481 tggtcgccat gaccgccggc gataccttcg ttgccaccgc gtttgtagtc atcggcgttg
     3541 ttaccgccat tacccgtgcc attgaccccg cggaagaccg cttttggatg ggggatgggt
     3601 ttgggctttt cgggaagggt ctcgggtact ttcaccggct tgaccttcac ccttgtaact
     3661 tctttatcgg ggatctttgt cgcgtcgggt ttggtgacga cggccttttt tacgacgggt
     3721 gcgtcttcct tttggttgtc atcggtctcg acgtccttga cgggttcttt ttcgacgacc
     3781 gcctgtttgg gaggtgtgta tttttccttg tcctggggcg aggggtcgcc cgggagatag
     3841 ggttgatctt tgccgagtcc ctgattactg ttgcccagat tcacttcaat cccttcttcg
     3901 acgacgggtt gcggctcggc cggtctcgtc cagctcaaac cgatgagcag aatgagcagc
     3961 gcggcgcaga cgacagtagt gtagcctgtc gctttcatgt ttttcctgga ttcgaattgt
     4021 tcggacatag gagctttttt ccgccgttgg cggaacgtcc gccaggcaat tcaattcctc
     4081 aaatttacca ctttctttgg gtgtgaatgc ataaaaatgt taaaatgcgg aacgatggcc
     4141 attgtggcgt ttgtgacgcc ggtagcgcag gggataattg atgtcatagg ccatttcgta
     4201 accggcgttg gcgacgaaat cctttctttt ttggcggttg cgtttgctga taaaaaagag
     4261 cgtaacacct gcggcgaggg tagtagtaag ggcggcgagc gcgatagctt gagatctctt
     4321 cattgttcga actttgtcaa atagatgcaa tagcaatgcc aaaagcgagt gacggcgcgg
     4381 gcagggttca gccgagccgg tgaagcattc ggagggcagg gttcagccga gccggtggta
     4441 aagccacgag ggaatatatc tgaaaagctt tgctatgatc caccagcggt gggttatata
     4501 gacgcgccag cgtttttttt cgatggcccg aacgatctgg cgggccgctt tgtcgacggg
     4561 agacacccag aacctgggac ccttggccat tttggtgtcg acgaaaccgg gctggatatc
     4621 ggtgatgtgg atggggatcg caagtttacg cgtcttgata tagagtccct cgaaataggt
     4681 gctttgaaag gccttggccg cgctgtatgc gggagagtgg cggtttccgc ggttggccgc
     4741 gatagaggat acggtgacga gatgaccttg tccctgctcc aggaaatact gccatccgaa
     4801 atggatcgtc tccaggaagg cgttgacatt gatatctaca gtggttttat cgacctgcca
     4861 gtcgagggga tccgtcaggt ctccccagcc ggcattgaag atcagcagat ggagcccgcc
     4921 cattttgcgg accagcgagg aaaggtgggt ggtcgcaccg ggtccggtgg cgtcatagca
     4981 ttcggtgacg atattgttgg gatattccag ctggagagag tataacaatt cctgccggcg
     5041 accggttgcg ccgacaagat gtcccgcggc agaatagagt cgggccagtt cacggccgat
     5101 gcccgaagtc gctcctacga tgatgatctt ttgcattagt gcgcgaaatc cagttcgtat
     5161 ttacctttga tacgttcaat gggcggattg aaataaccat acctgaggcc ggggaactct
     5221 tcttttatca gctgcatcag ctgtcgcacc cccatgcatt cgccggtatc ggtgacaccc
     5281 agccggccga gataccagtc ggggtcgatg ttgaaattcc tccactggat catgttcaga
     5341 ccggtggtgc ggatcaattt ccggagcgcc tcatattcgg cgatgctatc ggtcattccg
     5401 gggaacacga agtagttgat ggatgtccaa ccgccataat tacggactac gcggagactt
     5461 tccacgatat cgtcgaatgt gtagttatta ggtcggtaat atccttcata tatcggggcc
     5521 cgtgccgagt tcgtgctcac ccggatagag tcgagaccgg cttcgcagag tgcctgtacg
     5581 gcagcgggtt tgctgccatt cgtattgata ttgatgctgc cgcggtcggt atgtttccgc
     5641 atcccgagga tggcctcgcg gatggtctcc cacatgagaa gaggttctcc ttcgcaaccc
     5701 tggccaaagc tgacgatcgg gtagggggct tcctgcagat ggggtacggt gaactcgacg
     5761 atctcttctg ccgtaggctt gaaggtcaac ctgtcctggg ttgatgggat ggtctcttcc
     5821 tgcggctgga aagaaatgca gccgatgcag ttggcattgc atgccggcga gcttgggacg
     5881 ggacattccc agcgtcccat gaagaaattg cgggcggcgg ggcagtggta ggtcagacag
     5941 caattgtccg ccaaatggcg aacaaggcga ttatgcggat aggcggagcg cagtcgatcg
     6001 accccattgt tgatgacatt ccagtcatag ccggcacagt cctggcggat atcctgttcg
     6061 atgcgcaggg cggggacgaa gaatttgccg tcctgccagc cggccgcggt atagcagaat
     6121 agcggcaggg tcggcgcgcc gggagtcgtt tcataggctg cgaggaaaag accggtatgg
     6181 gcgggaggga tgaaggctgc tactgcccag cctttctcgc aaagccgcat ttcgccggtg
     6241 cgtacatcga tgccgatgcc tctcctgccg ggcaattcat acaggctgcc tccgtcgggt
     6301 agtgcgatcc aatcctgtgg gtcgatgggg agggcgtccc aaccgctgcg accggtggag
     6361 tgcagtgtgg tgtcttcgaa gatattgcct ttcccgtccg agtagaggat atacggtgat
     6421 tccttcagca tccgcaaatg tacgacaaat gcggtgaacc ggggggcggt gaggggcgtg
     6481 gatcaggcga gccgggcagt gaggggagtg gatgaggcga accggggccg acgcgggggc
     6541 tgactcaggg cttgccgaga tagaactggc aaaaggcctg tatccgctcg agttcttcga
     6601 actgcagatt gcggaggaat tcaaagcgaa ggtctttgtc gtccgaggtg caaatatcga
     6661 tggagtcgta ggtagccgtg aggtcattga tatgcgtcca gtaaaggaaa cggctttcgg
     6721 gtatatccgg aatagtgatt ccggtatggt ggatcgagag ggtctcttcg gaacggaggc
     6781 ttcgttcgta atagaataga tagcagtaac ccgcggtcag gaggagatgg aagatccccg
     6841 tcatcgcgtg gtgttctacc agcatgagca ggccgatgcc caggaagaaa atgatctcga
     6901 ccgtgcggaa aaagagattg acgcggggcg ttcgtttcag gatgtcccga cctacaagca
     6961 ccagcaggaa gatatcgatc gaaatgagca actggcacca gaagtaaagg gtgtgcctgg
     7021 attcaccgcg gaaatggctg atcgcgtggg caaggatcag tgcgccggcg aggaggtgta
     7081 gcccagcccc attggttttg agacgttgac attttggcgc g
//
LOCUS       DASYSV010000414         6675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_209115, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000414 DASYSV010000000
VERSION     DASYSV010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209115"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="VGQ51_12750"
     CDS             complement(<1..1912)
                     /locus_tag="VGQ51_12750"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV9037492.1"
                     /translation="MSVNIQPFLRLAGLEPLILRPQTNFVNVGERTNVTGSKKFARLI
                     RDNKYEEALSVARQQVDSGAQILDVNMDDALLDGVQAMQTFLNLLQAEPDIAKIPIMI
                     DSSKFSIIEAGLKCVQGKCIVNSISLKEGEEKFIEQAVICRSYGAAVIVMAFDENGQA
                     DTLRKKVDISERAYRVLTEKVGFDPEDIIFDPNIFAIATGIEEHNNYGLDFIEAIKEI
                     KKKMPLTRISGGVSNVSFSFRGNEPVREAMHSVFLYHAIRAGMDMGIVNAGQLVVYDE
                     IEPQLRQLCEDVILNRNAEATDKLLAFAETVKAKGKEEVKDEAWRRGTVEERLKHALV
                     NGITDYIDADTEEARLKYPKPLAVIEGPLMAGMDVVGDLFGSGKMFLPQVVKSARVMK
                     KSVAVLTPYIEQEKEERKKNAGAAAEETAGAARILLATVKGDVHDIGKNIVGVVLGCN
                     GYDVIDLGVMVPADKILETAKKEKVDVIGLSGLITPSLDEMVHVAREMKRQDFKIPLL
                     IGGATTSRMHTAVKIAPEFGNGVVHVLDASRSVTVVGSLLNPGQKPEFLATVKKEYQK
                     LKEDFAARKTVKQFLPFGQAQENKVPVDWNAFRPVTPTFIGTKVFDGYDLAEIVRYID
                     WGPFFIAWELHGK"
     gene            complement(1909..2940)
                     /locus_tag="VGQ51_12755"
     CDS             complement(1909..2940)
                     /locus_tag="VGQ51_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEV9037493.1"
                     /translation="METTRKLLEEALRKRILVIDGAMGTMIQRHKLEEKDYRGQRFSD
                     WPSDLKGNNDLLCLTQPAIIEDIHRQYLEAGADIIETNTFNAQRISMADYHMEDVAFE
                     INVAAATIAKKAAADYTARNPATHRFVAGAIGPMNKTLSLSPDVNNPGFRAVSFDEVA
                     SAYYEQVKGLVEGGVDLLLIETIFDTLNAKAAIFAIKKYFRDAKRPELPISISGTITD
                     ASGRTLSGQTLEAFYVSVMHARPLSVGLNCALGASEMRAHIEELSQIAACFISAYPNA
                     GLPNAMGEYDEQPEQTAHFIEDWAENGFVNIVGGCCGTTPDHIRHIAQHVASIRPRPL
                     PTVEKELAL"
     gene            complement(3155..3775)
                     /gene="recR"
                     /locus_tag="VGQ51_12760"
     CDS             complement(3155..3775)
                     /gene="recR"
                     /locus_tag="VGQ51_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV9037494.1"
                     /translation="MQFSSSLLEQAVGEFSRLPGIGKKTALRLVLHLLKQDAGQVQGL
                     GETLIRMRNEIKFCQRCHNVADADVCSICANSLRKQELICVVESLREVIAIESTQQYN
                     GTYHVLGGSISPLDGVGPDQLNIETLVHRVRKEQTQELIFALNPTIQGDTTIYYIQKK
                     LQDTPVRITTIARGIAFGGELEYADEMTLARSITNRLPVESYRAGG"
     gene            complement(3846..5285)
                     /locus_tag="VGQ51_12765"
     CDS             complement(3846..5285)
                     /locus_tag="VGQ51_12765"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HEV9037495.1"
                     /translation="MKINNGLPFPILFILVLIGAVGHSGCANIIPPTGGPRDTIPPVL
                     VSSVPKDSVKRFTEKKIILNFNEYIDGKDIRTELLVNPVPKIDPIVDAKLRVVTIRLK
                     DTPQAHTTYSLQFGKGIKDVNEGNILKNFTYVFSTGDTIDRGELEGEVIIANTGRPDS
                     TLVAILHASFDDSAVIKDRPRYIARLDSTGRFHFRYVKPGKYALYALKDEGGSHKYLS
                     KAQLFAFAEGPVIVGETGTAQTLYAYAEIAETKKSGTTGTTGTTPPPPKKPKKEDTRL
                     QVTTNAGNGQFDVLDTFRLSFSSGLKVFDSVQLRFTNEDFQDIDGKQYRWIRDSTERI
                     VTLQYAWPTDVRYHIIIPRTFGQDSLGRKLLQDDTISFHTKKDIDYGEVLVRVKNLST
                     TLNPVLQFVQADKVKYSFPFNSRGIVRKLLFPPGEYELRILYDINHNGVWDPGSFFGG
                     AKKQPEKVITIRKKFNIKANWDNDWDTTL"
     gene            complement(5384..>6675)
                     /locus_tag="VGQ51_12770"
     CDS             complement(5384..>6675)
                     /locus_tag="VGQ51_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEV9037496.1"
                     /translation="PLVQVSGSDKAVNLLTAHGSKGLEFEYVFFAGLNSATWEKKRKP
                     GGGYKYPDNLLSGAGGGESSAEEELRRLFYVALTRVEQHLFLSYSRFRDDGKELEPSV
                     FIAEILQDHDLPFEKATIPAEDQASFDALPFADAIAPQIRADENEFVDRMLEKFVMNV
                     TALNNYLKCPLEFYYRNLLRIPSPKNEATEFGSAVHFALQRLFEKMKEHPSERWPAVE
                     EVLDDFQWYMRRHREIFTKEAFARRMEYGLEVLTNYYNKYLPGWNKIVAVERNIRNVT
                     VRGVPLKGKLDKLEFHGKEVNVVDYKTGDVDKAKEKLSGPGEKDPNGGDYWRQAVFYK
                     LLVDGYEQKDWRVVSTEFDFVEPDKKKEYRKAKIMIGEEDTTTVTQQIVRTWQSIRNR
                     EFYTGCGKPDCHWCNFVKTNNLAIALHEIAEHEEEEI"
BASE COUNT         1387 a         1862 c         1711 g         1715 t
ORIGIN      
        1 attttccatg cagctcccag gcgataaaaa agggtcccca gtcgatgtag cggacaatct
       61 cagcgagatc atatccgtca aagaccttcg tgccgataaa ggtcggcgtc acgggccgga
      121 aggcattcca gtcgacaggc accttatttt cctgcgcctg gccgaacggt aggaattgtt
      181 ttaccgtttt cctggcggcg aaatcttcct tcagtttctg atattccttt ttcaccgtcg
      241 caaggaattc cggcttttgc ccgggattga gcagtgaacc caccaccgtg acactgcggg
      301 atgcgtcgag gacgtgtacc accccattcc cgaactcggg cgcgatcttc actgcggtgt
      361 gcatacgcga agtcgtcgcg ccgccgataa gcaggggtat cttaaaatcc tgccgcttca
      421 tttctcgtgc gacatgcacc atctcatcca gcgaaggcgt gatgagtccg ctgagaccga
      481 tgacatccac tttttccttt ttggcggtct ccagtatctt gtccgcgggt accatcacac
      541 cgagatcgat gacatcatat ccattgcagc ccaggacgac gccgacgata ttcttcccga
      601 tgtcatgcac atcgcccttc acggtggcca gcagtatcct ggcggcgcct gccgtttctt
      661 ccgccgccgc tccggcattc tttttccgct cttccttctc ctgctcgata tatggggtca
      721 gcacggctac cgatttcttc atcacgcgcg cgctcttcac cacctgcgga aggaacatct
      781 tgccgctgcc gaacaggtct cccacgacgt ccatgccggc catcaatggt ccttctatga
      841 cggccagtgg tttgggatat ttcagccggg cctcttccgt gtcggcgtcg atatagtcag
      901 tgataccgtt gaccagcgca tgtttcagcc gttcttccac cgtgccgcgg cgccaggctt
      961 cgtcctttac ttcttctttt cccttggctt tcaccgtttc cgcaaaggcc agcagcttat
     1021 cggtggcctc ggcattcctg ttcaggatga catcctcgca cagttgtcgt aattggggct
     1081 ctatctcgtc atagacgacc agttgacccg cattgacgat acccatatcc atacccgccc
     1141 ggatggcgtg atagaggaat acgctgtgca tggcttcccg caccggttca ttaccgcgga
     1201 aggagaagga aacattgctg acgccgccgc tgatccgggt caatggcatc ttttttttga
     1261 tctcctttat agcctcgatg aaatccagac cataattatt atgctcttcg atgcccgtgg
     1321 cgattgcgaa gatattcgga tcgaagatga tgtcttccgg gtcgaatcca accttttccg
     1381 tgagtaccct gtatgctctt tcgctgatat ccaccttttt tcgcagcgta tcggcctgac
     1441 cgttctcatc gaaggccatg acgataacgg cggcgccata gctccggcag atcaccgcct
     1501 gttcgatgaa cttctcttct ccttctttca gcgaaatgga gttgacgatg catttcccct
     1561 gtacgcattt caacccggct tcgatgatcg agaacttgga ggaatcgatc atgatcggga
     1621 tcttcgcgat atcgggttcg gcctgcagca gattgagaaa ggtctgcatg gcctgtacgc
     1681 catccagcag ggcatcgtcc atgttcacat ctagtatctg cgctccgcta tctacctgct
     1741 ggcgcgccac gctgagcgcc tcttcatact tgttatcgcg gatcagccgc gcgaactttt
     1801 tcgaaccggt gacattggtt cgttcgccga cattgacaaa attcgtttga ggacggagga
     1861 tcagaggttc caggcccgca agacgcagga agggttggat gttcacgctc ataatgctaa
     1921 ttctttttcg acggtcggta agggccgcgg tcggatggaa gcgacatgct gcgcaatatg
     1981 ccggatatga tccggggtcg ttccacaaca gccgccgaca atattaacga atccattttc
     2041 cgcccaatct tcgatgaaat gggcagtctg ttcgggttgt tcatcgtatt cgcccatcgc
     2101 gttcggcagt ccggcgttgg gataggcgga gatgaaacat gccgcgatct ggctgagttc
     2161 ttcgatatgg gctctcattt cactggcgcc cagggcgcaa ttcaaaccta cgctcagtgg
     2221 acgggcatgc atcacggata cataaaaggc ttccagcgtt tgtccgctga gcgtccggcc
     2281 gctggcatcg gtaatggtcc cgctgatgga tatgggtaat tcgggccgtt ttgcgtcccg
     2341 gaaatatttt ttgattgcga agattgcggc ctttgcgttc agcgtatcga agatcgtttc
     2401 gatcagcagg agatcgacgc cgccttctac cagacccttt acctgctcgt aataagccga
     2461 tgccacttca tcgaagctga ctgcgcggaa acccggatta ttgacatcgg gagaaagcga
     2521 aagggtcttg ttcatcggtc cgatggcccc ggctacgaat ctatgcgtgg caggattgcg
     2581 ggctgtatag tcggccgccg cctttttggc gatggttgcc gccgcgacgt tgatctcaaa
     2641 ggccacgtcc tccatatgat aatcggccat gctgatccgt tgagcattga aggtattggt
     2701 ctcgatgata tcggcgccgg cttcgagata ttgccggtga atgtcttcga tgatcgcagg
     2761 ctgtgtcaga cacaggaggt cgttattgcc tttcagatcg gaaggccaat ccgaaaagcg
     2821 ctgaccacga tagtcctttt cctccagttt gtgccgttgg atcatagtcc ccatggcccc
     2881 gtcaatgacg agaatacgtt tacgcagggc ttcttccaat aattttcgtg ttgtttccat
     2941 gtgttattcc tccttttttg caaaccgaac gactaaaacg agggaaataa ccggaaagag
     3001 gtgggatatt tcaaacgttt attagttcag tttttaacga gccgcaacag gccgaacaat
     3061 aaacaaatct gcctgtacgg gtgcaaagat acgcaaaaaa acaattaaat acaatggctt
     3121 gagcctgcct cgccacgcgt gagaccctgc tgccctaacc acccgcccga tagctttcaa
     3181 ccggcagacg gttggtgatc gacctcgcca gggtcatttc atcggcatat tccagctctc
     3241 cgccgaaagc gataccccgc gctatcgtcg tgatccgtac aggtgtatcc tgcagctttt
     3301 tctggatata atagatggta gtatcgccct ggatcgtagg gttgagggcg aagatcagtt
     3361 cctgcgtttg ttccttccgg accctgtgga cgagggtctc gatattcagc tgatccggcc
     3421 ctacaccatc cagcggggaa atgctgccgc ccagcacatg ataagtgcca ttgtattgct
     3481 gcgtgctctc gatggcgatc acctcgcgaa gactttcgac gacgcagatc agctcctgtt
     3541 tgcgtaagga attggcgcag atggaacaga cgtccgcatc ggctacgtta tgacagcgtt
     3601 ggcagaattt gatctcgttg cgcatgcgga tcagcgtctc acccaatccc tgcacctgac
     3661 cggcatcctg tttcaacaaa tgcaacacca gacgcagggc cgttttctta ccgatgcccg
     3721 gcagccttga aaattcaccc acggcctgtt ccagtaaaga ggaagagaat tgcatccggc
     3781 aaagatacga aacaggcgcc atcgggccgc cagccagcgc atcccgatcg ccccgtggcg
     3841 ccgacttaca gcgtcgtatc ccagtcgttg tcccagttgg ctttgatatt gaatttcttc
     3901 cggatggtga tgactttttc aggctgtttt tttgcaccgc caaagaacga tcccgggtcc
     3961 cagacgccat tgtggtttat gtcgtagagg atccgcagtt catactcccc gggcgggaat
     4021 agcagcttgc ggacaatccc ccggctgttg aaggggaacg aatatttcac tttgtccgcc
     4081 tgaacgaatt gaaggaccgg gttcagcgta gtcgataaat tctttacccg gaccaggacc
     4141 tccccgtaat ctatatcttt tttggtgtgg aaactgatcg tgtcgtcctg cagcagcttc
     4201 cttcccagcg aatcctggcc gaaggtcctg ggaatgatga tatggtaccg gacatccgtc
     4261 ggccaggcat attgtagggt aacgatcctt tcggtggagt cgcggatcca ccggtattgt
     4321 ttgccgtcga tgtcctgaaa atcttcattg gtgaaccgga gctggacgga atcaaagact
     4381 ttcagtccgc tggaaaagct cagacgaaac gtatccaaaa cgtcgaattg accattgcct
     4441 gcattcgtcg tgacctggag cctggtatct tctttttttg gctttttggg aggcggcggg
     4501 gtggtacccg ttgtgcccgt ggtcccgctc tttttggtct cggcgatctc cgcataggca
     4561 taaagggtct gggccgtgcc ggtttcgcct actatcacgg gtccctccgc aaacgcaaaa
     4621 agctgtgcct ttgaaagata cttgtggctt ccaccctcat ctttcagcgc gtaaagggcg
     4681 tattttcccg gtttgacata ccggaaatgg aatctgccgg tgctgtcaag tcttgcgata
     4741 tacctgggcc ggtccttgat gacggccgaa tcatcgaaac tggcatgcag gatagccaca
     4801 agtgtgctgt ccggcctgcc tgtattggcg atgatcactt ccccctccag ctcgcctctg
     4861 tcgatcgtgt caccggtaga gaagacataa gtaaaattct taaggatatt gccttcattg
     4921 acatccttga tgcctttccc gaactgcaga gaataagtag tatgcgcttg cggagtgtct
     4981 ttgagacgaa tggtcaccac cctgagcttg gcgtcgacta tcgggtctat cttcggcacc
     5041 gggtttacca gcaactcggt tctgatgtct tttccgtcga tatactcatt aaagttcagg
     5101 atgatctttt tctcggtgaa acgtttgacc gagtctttcg gtaccgagga aaccaggacg
     5161 ggcgggatcg tatccctggg acccccggta ggaggtatga tgttcgcgca gccggaatgc
     5221 ccgacggcgc ctatgaggac caggatgaac aggatgggga aagggagacc gttattgatc
     5281 ttcatggtta aatgattggc tttcggccct ttggcctccg gcccattttt gcaaacatag
     5341 ttcgtttaag tgtaagcata tcccgggtga tcctaatatt ccgctaaatc tcctcctctt
     5401 catgttccgc gatctcgtgc agggcgatgg ccaggttgtt cgtcttcacg aaattgcacc
     5461 aatgacaatc cggcttgccg cagcccgtat agaattcgcg gttccggatc gattgccagg
     5521 tccggacgat ctgttgcgtg acggtggtcg tgtcctcctc tccgatcatg atcttcgctt
     5581 tccggtattc cttcttctta tccggctcca cgaaatcgaa ttcggtgctc accaccctcc
     5641 aatccttttg ctcgtaaccg tctaccaata gtttgtagaa cacggcctgg cgccagtagt
     5701 cgccgccgtt aggatctttc tcgccgggac ccgacagttt ctccttcgcc ttatccacat
     5761 cgccggtctt ataatcgacc acgtttactt ccttcccgtg aaattcgagt ttgtcaagct
     5821 tgcctttaag gggaacgccc cgcacggtga cgtttcggat attccgttct acagcgacga
     5881 tcttattcca tcccgggagg tatttattat agtaattcgt cagtacctcg aggccatatt
     5941 ccatccgcct cgcgaaggcc tccttcgtaa agatctcccg gtggcggcgc atataccact
     6001 ggaaatcgtc cagcacttct tccacagcag gccaacgctc cgaaggatgt tccttcatct
     6061 tctcgaaaag acgctgaagg gcgaaatgta cggcgctgcc gaattccgtg gcttcattct
     6121 tgggagaggg tatacgcagc aggttccggt aatagaattc cagcgggcat ttcaggtaat
     6181 tgttcagtgc cgtcacgttc atgacgaact tctccagcat ccggtcgaca aactcatttt
     6241 catccgcccg gatctggggc gctatcgcat cggcaaaagg aagggcatcg aaggatgcct
     6301 ggtcttccgc cggaatggtc gccttttcga agggcagatc atgatcctga aggatctccg
     6361 caatgaagac cgaaggctcc agttccttac cgtcgtcccg aaagcgggaa taggacagga
     6421 aaaggtgttg ttcgaccctc gtcagcgcca catagaaaag acgacgcagc tcttcttctg
     6481 ccgaggattc gccgcccccc gctccgctca acaggttgtc gggatattta taccctccgc
     6541 ccggcttgcg tttcttttcc caggtggccg aattgagacc tgcgaaaaaa acgtattcga
     6601 actcgagccc tttcgaccca tgtgccgtca gcagattgac cgccttatcg cttccggaaa
     6661 cctgcaccag cggca
//
LOCUS       DASYSV010000415         6643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_856156, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000415 DASYSV010000000
VERSION     DASYSV010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856156"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2491
                     /locus_tag="VGQ51_12775"
     CDS             <1..2491
                     /locus_tag="VGQ51_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEV9037497.1"
                     /translation="INRHNGTFQDQLNDRVMHTSQYTMGVDIADADNDGFPEIVTMDM
                     LPYDPYILKRSEGEDTWDVFNLKIGYGYNYQYTRNNLQYNRRNGHFSEIGLYSGIAAT
                     DWSWSPLWVDFDNDGLKDLFISNGIPKRLNDIDYINFISNEELQRKIQDRSMSDKDID
                     LINKFPEIKLPSRFFRNKGQMSFSNMDDSIDGIHPTYSNGAIDADLDGDGDLDLVVNN
                     IDEPVLVYQNLQSEKARRPAVDVTLKGPEKNINALGAKIFVFSKGDIRSYEKYPVRGF
                     LSSAEIPLHIGLYQTPVDSAFLVWPDNSYQRIDLPAHTGHMTFSYTKGLPLFDYRIVT
                     DHWKNPSAPVKDITQSTRLLYRHVENNFHEFDREPLLPHMLSTEGPATAIADINHDGL
                     EDVFIGASKWQKPAVFIQDKAGKFGRLPEPALDNDSTYEDVDACWTDVNNDGAPDLVV
                     ASGGNEYYGQEPWRSPRVYLNDGKGSLTRLDKAFSDLYLNASCVAPSDFDHDGNIDLF
                     IGGRSVPFDYGKIPRSYLLRGDGKGHFTDVTTQVAKELAGIGFVTSAQWVDLDKDGRK
                     DLLLTLEWGGIVAFLDKDGHLTKKVLSDKKGWWNFVLPVDLDGDGNLDLIAGNLGLNS
                     RLKASDQKPVRLYYADFDGNDKKEQILTYYIGDRELPFANKHELEMQLPSIKKNFLYA
                     QDFAKASLQDIFSVDHLRQADTLTANYFSNAILINDGHLNFRLQAMPWEAQLSSYRTA
                     VVVNANGDSLPDILLAGNYYDNNIQMGRYDADYGTLLLNHGRDSFTTTPLNGLAIEGQ
                     VRHILPLRVAGKQAFLLTKNNDSARVIQFQP"
     gene            complement(2557..3492)
                     /locus_tag="VGQ51_12780"
     CDS             complement(2557..3492)
                     /locus_tag="VGQ51_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037498.1"
                     /translation="MKRLLVIVVLLVLVVLVCIEVFIPSTVKVSPVVPVRSRAAAIFR
                     VLRNEDRWMGWWPNSSMGLDNGELWYHGLGYRVEKLSYNIVDVGIRTSSEELESRISI
                     IPVGSQDSSLLQWSCDIPVGSNPLRKIGRYAEARKIASAMKELLGFAADYLGKKENIY
                     GVSVGESSTRDTLLVAEEGHFRGYPGNADIYGLVNAVKKRIADAGGKVTGYPMVNISG
                     GDTGYKVRVAVPTDRNIPAVGRFYFMRLIPGNYLVTAVTGGQGAVNNALEGMKEYISD
                     YQRTVMAIPFQSLVTDREEEKDSTKWKTRIYYPIF"
     gene            3542..5593
                     /locus_tag="VGQ51_12785"
     CDS             3542..5593
                     /locus_tag="VGQ51_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEV9037499.1"
                     /translation="MTAQKKLSLLFLLLIITHTLPAQTSDPQPDTWTIHADSIDPNNY
                     YGVTVANGMLGLVSSTDPFKAKDIVLNGAFDLYGRGRVSNILKTFNFINLYLDIDDHR
                     IGSFKEVTHLRQTLDMRHACLTTSFDYKDKATITYTWYSLRQLPYTALVDVTITAHKD
                     ISIVPASVMEAPDMLRDVENYNNDIDRPHVKISLLTSTAKSPTGKLLMAASNSFIFDE
                     PHPNEPITIHEMWDNNMHLLKFKKKLQAGTTYHFAVVGSSLTSAHNDDPLNEAERLTI
                     FAALQGAKSLIHDHEQAWDALWKSDIVIDGDAQTQRDIRLMLYHLYSFAREGTSYSLS
                     PMGLSGLGYNGHTFWDTEIWMYPALLLLQPKIAASLMEYRYQRLPAAIRNASEHGYKG
                     AMFPWESAASGNEETPVWALSGPFEHHITADVGIAAWNYYCVTRDISWLKEKGWPLLQ
                     ATADFWASRVQRNGPGHYDIDNVVAADEWAENVDNNAYTNAAARTNLQDAIEAAHVLG
                     IRADPDWNNVRNNIPILRFPDGTTREHAAYNGEPIKQADVNLLAYPLKEVTTPAAIRK
                     DLEYYEPRVGEGPAMTHAIFSILYERLGQPDKALKVLKEGYIPNLRPPFGVIAETASG
                     TNPYFATGAGGVLQALLDGFGGLDITADGIVQLPTHLPAGWKSLRITGVGLDHTTYTV
                     K"
     gene            5671..6513
                     /locus_tag="VGQ51_12790"
     CDS             5671..6513
                     /locus_tag="VGQ51_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEV9037500.1"
                     /translation="MGQFFVSFRKAFRNLYVLTFLNGFLIACLLFFHILSVYENGLFD
                     SIKSSIDATANGGATPDSILIKSMHACHELMHSRADVFIGNTRRLGPEGGILHSAAVD
                     LNTTDGACGSYSEVLARVLRSYNYPIRIAQMMAGGKYGAHMVVEAYDGSRWVALDPSY
                     NVFYVRPDGQLASIADIRGNWSYYRNQTPPDYDQAYRYEDVRYTNWSKIPVLMPAIKK
                     VLTWIIGSERTNHLSLRVLFLHVYDIYFYITLILEIALLLGTIRIQIRIRRNPAPLHL
                     EPSV"
BASE COUNT         1640 a         2241 c         1381 g         1381 t
ORIGIN      
        1 tatcaaccgg cataacggca ccttccagga ccagctgaac gaccgcgtca tgcacacaag
       61 ccagtatact atgggcgtgg atatcgccga cgccgacaac gacggcttcc ccgagatcgt
      121 caccatggac atgcttccct atgatcccta catcctcaaa cgttccgaag gcgaagacac
      181 ctgggacgtc ttcaatctca aaataggcta cggctacaat tatcaatata cccgtaacaa
      241 tcttcagtac aaccgccgca acggacactt cagcgagata ggcctgtact cgggtatcgc
      301 cgctaccgat tggtcatggt cgccgctatg ggtcgatttc gacaacgacg gtctcaaaga
      361 ccttttcatt tccaatggta tcccgaaaag gctcaacgac atcgactata tcaatttcat
      421 ctccaacgaa gagctccagc gaaagatcca ggaccgaagc atgtcggata aggatatcga
      481 cctgattaac aaattccccg agatcaaact gcccagccgg ttctttcgca acaaaggaca
      541 gatgtccttc tccaatatgg acgacagcat cgacggcatc catcccacct attccaacgg
      601 cgctatagac gcggacctgg acggcgacgg cgatctcgat ctcgtcgtca ataacatcga
      661 tgaacccgtc cttgtctatc agaacctcca gtcggaaaag gcccgccgcc ccgcggtaga
      721 cgttaccctg aaaggacccg aaaagaacat caacgccctc ggcgccaaga tctttgtctt
      781 ctccaaagga gacatccgca gctatgaaaa atatcccgtc aggggcttcc tgtccagcgc
      841 ggaaatacct ctccacatcg ggctgtacca aacacccgtc gattcggcat tcctggtatg
      901 gcccgacaat tcctaccaac ggatcgatct gcccgcccac accggccaca tgaccttcag
      961 ctacacaaaa ggcctgcccc tgttcgatta ccgtatcgtt accgatcact ggaaaaaccc
     1021 gtcggctccc gtaaaagata tcacccaatc gacccggctg ctctatcgtc atgtcgagaa
     1081 taacttccat gaattcgacc gggaacccct gctcccgcat atgctttcta ccgagggtcc
     1141 cgccacggcc atcgcggaca tcaaccacga cggtctggaa gacgttttta tcggcgcctc
     1201 caaatggcaa aaacccgccg ttttcatcca ggacaaagcc ggaaaattcg gacggcttcc
     1261 cgagccggcc ttagacaatg acagcaccta cgaagacgtc gatgcctgct ggaccgacgt
     1321 caacaatgac ggcgcccccg atctcgtggt ggccagcggc ggcaacgaat actatggcca
     1381 ggagccatgg cgctctccgc gggtatacct caacgatggg aaaggctcac tcaccaggct
     1441 cgacaaggcc ttctccgatc tttacctcaa cgcgtcctgc gtcgccccct ccgatttcga
     1501 tcacgacggt aatatcgatc tgttcatcgg aggcagatcc gttccttttg attatggcaa
     1561 gatcccccgc tcgtacctgc tcaggggcga tggcaaaggt catttcaccg atgtcaccac
     1621 acaagtcgcc aaagaacttg ccggtatcgg cttcgtcacc tctgcgcaat gggtcgatct
     1681 cgataaggac ggccgaaagg acctgctcct gacgctcgaa tggggcggca tcgtcgcctt
     1741 cctcgataaa gacggccacc tcacaaaaaa agtgctctcg gataagaaag gctggtggaa
     1801 tttcgtgctg cccgtagacc tcgatggcga tggcaacctc gatctcatcg caggcaacct
     1861 cggcctgaac agccgcctca aggcatcgga ccaaaaaccg gtccgtctgt actatgccga
     1921 tttcgacggc aacgacaaaa aagaacagat cctcacatac tatatcggcg accgcgagct
     1981 ccccttcgcc aacaaacacg agctcgaaat gcaattgccc tcgataaaaa agaatttcct
     2041 ctacgcccag gatttcgcaa aggcgtcact acaggacatc ttctctgtcg atcacctccg
     2101 ccaggccgat acgctgacgg caaattattt ctccaacgcc atccttatca atgacggtca
     2161 tctcaatttc cgcttgcagg ccatgccctg ggaagcccag ctctcctcct atcgcaccgc
     2221 ggtagtcgtc aacgccaacg gcgatagcct ccccgatatc ctcctcgcag gcaattacta
     2281 tgacaacaac attcaaatgg gaagatacga cgccgattac ggcacacttc tcctcaacca
     2341 cggccgcgac tccttcacga cgacgcccct caacggactc gccatcgaag gacaggtccg
     2401 ccatatcctg cccctccgcg tcgccggcaa acaagccttc ctcctcacaa aaaacaacga
     2461 cagcgcccgc gtcatccaat tccaacctta acgtccctcg tcatccagtt cacgccctaa
     2521 caacccgcgt catccggccc tggcgccccg cgccgctcaa aatatcggat aatagatcct
     2581 cgtcttccac ttcgtactat ccttctcctc ctccctatcc gtcaccagcg attggaaagg
     2641 tatcgccatc accgtccgct gataatcgct gatatactcc ttcattccct ccaacgcatt
     2701 attcacggcc ccctgccccc ccgtcaccgc cgtgacgaga taattccccg gtatcagcct
     2761 catgaaataa aagcgcccca cagcaggtat attcctgtcg gtaggcaccg ccacccgtac
     2821 cttataaccc gtatcccccc cggatatatt caccatcgga taacccgtca ccttcccccc
     2881 cgcgtccgcg atccgcttct tcactgcatt aaccaaccca tagatatccg cattccccgg
     2941 ataaccgcga aaatgtccct cctccgccac caacagcgta tccctcgtcg agctctcccc
     3001 aacggataca ccatagatat tctccttttt acccagataa tccgccgcaa aacccaataa
     3061 ttccttcatt gccgaagcga tcttcctggc ctccgcataa cggccgatct tgcgcaaagg
     3121 attgcttccg accgggatat cacaactcca ctgcaacagc gaagagtcct ggctccccac
     3181 cggaataata ctgatcctgc tctccaattc ctccgacgac gtccggatgc cgacatccac
     3241 gatattatag gacagctttt ctacacggta gccaagcccg tgataccaca attccccatt
     3301 atccaatccc atcgacgaat tgggccacca ccccatccac cggtcttcat ttcgcagcac
     3361 acgaaaaatc gccgccgccc gactccgcac cgggaccacc gggcttacct tcaccgtcga
     3421 gggaataaat acttcaatac acacaagcac caccagcacc aaaaggacaa caataaccaa
     3481 caatctcttc atatagacaa taatacgtaa tttcgacacc aacgattgca aatttgcgac
     3541 tatgaccgca caaaaaaaac tctccctcct ttttctactc ctcatcatca cccataccct
     3601 cccggcccaa acatctgacc cccagcctga cacctggacc atccacgccg actccatcga
     3661 ccccaacaac tattacggag tcaccgtcgc caatggcatg ctcggcctcg tatcctcaac
     3721 cgaccccttc aaagccaaag acatcgtcct caatggcgcc ttcgacctct acggccgcgg
     3781 ccgcgtcagc aatatcctca agaccttcaa tttcatcaat ctctatctcg atatcgacga
     3841 ccaccgcatc ggcagcttca aagaggtcac ccatctccgc cagaccctcg acatgcgtca
     3901 tgcctgtctg accacctcat tcgactataa ggataaagcc acaataacat atacctggta
     3961 ctcgcttcgc caactgccct ataccgccct ggtagacgtc accatcactg cccataagga
     4021 catctccata gtccccgcca gcgtcatgga ggctcccgac atgctccgcg acgtcgaaaa
     4081 ctacaacaac gacatcgatc gtccacacgt aaagatctcc ctcctcacat ccaccgccaa
     4141 aagccccaca ggcaagctcc tcatggcggc ttccaacagt ttcatcttcg acgagccgca
     4201 tcccaacgag ccgatcacta tccatgagat gtgggacaat aatatgcatc tgctcaaatt
     4261 caaaaagaag cttcaggccg gaacgactta ccacttcgcc gtcgtcggct cctcccttac
     4321 ctccgcccac aacgatgacc cgctcaacga agccgaacgt ctaaccatct tcgcggcact
     4381 gcagggcgcc aaaagcctca tccatgacca cgaacaagcg tgggacgcac tctggaaaag
     4441 cgatatcgtc atcgacggcg atgcgcaaac ccaacgcgat atccggctca tgctctatca
     4501 cctctattcc ttcgcgcgcg aaggcacgtc ctatagcctg tcccccatgg gcctcagcgg
     4561 tctgggctac aacggtcata cattctggga tacggagatt tggatgtacc cggcgctgct
     4621 cctgctacag ccaaagatcg ccgcctcgct tatggaatac cgttaccagc ggctcccggc
     4681 ggccatacgc aacgcctccg aacatggcta caaaggcgcc atgttcccct gggaaagcgc
     4741 cgccagcggc aatgaggaga cgccggtatg ggccctaagc ggtcccttcg aacatcatat
     4801 caccgccgat gtcggcatcg cggcatggaa ctattactgc gtaacccgcg acatctcatg
     4861 gctaaaggaa aaaggctggc cgctgctgca ggcgactgcc gatttctggg ccagcagggt
     4921 acaacgcaac ggccccggcc actacgatat cgacaatgtg gtcgccgccg atgaatgggc
     4981 cgaaaacgta gacaataatg cctacaccaa tgccgccgcc cgcaccaatc tacaggacgc
     5041 catcgaggcg gcacatgtcc tcggcatcag ggccgatccc gattggaaca atgtccggaa
     5101 caatattccc atcctccgtt tcccggatgg caccacccgc gagcatgccg catacaacgg
     5161 tgagcccatc aaacaggcgg atgtaaacct gctcgcctac cccctcaaag aggtcaccac
     5221 ccccgccgcc atccgcaaag acctcgaata ttacgaaccc cgcgttggcg aaggccccgc
     5281 catgacacat gcgattttct ccatcctcta tgaacgtctt ggccaacccg ataaggctct
     5341 aaaagtcctc aaagaagggt atatacccaa tcttcgtccc cccttcggcg tcatagccga
     5401 aacggcatcc gggaccaacc cctatttcgc caccggcgca ggaggagtgc tgcaagccct
     5461 gctcgatggt ttcggcggtc tcgatatcac ggcagatggc attgtacaac tccctaccca
     5521 tttgcccgca ggctggaaat cacttaggat caccggtgtc ggactggatc ataccacata
     5581 taccgtcaaa taattcacgt gaattcaatt ttggtcgtac cttcgttctg cccccaagtc
     5641 cgtatccgct tattaaccta ttaattcttt atggggcaat tcttcgtctc cttcagaaaa
     5701 gcatttcgaa atctatacgt gctcacgttt ctcaacgggt tcctgattgc ctgtctgcta
     5761 ttcttccaca tactctccgt ttacgaaaac ggactcttcg attccatcaa atccagcatc
     5821 gatgccactg ctaatggcgg cgccacccct gattccatcc tcatcaaatc catgcatgcc
     5881 tgccacgaac tgatgcatag cagagcggat gtctttatcg gcaataccag gcggctgggg
     5941 ccggagggcg gcatcctcca ttccgcggct gtcgatctga ataccaccga tggtgcctgt
     6001 ggcagctatt cagaggtgct tgcccgggtt cttcgcagct ataactaccc tatccgcatc
     6061 gcccaaatga tggcaggtgg caaatatggc gctcatatgg tggtcgaagc ctacgacggc
     6121 agccgctggg tggcgctcga ccccagctac aacgtgtttt atgtccgtcc ggacggccaa
     6181 ttagccagca tcgccgacat ccgcggaaat tggagttatt accgcaatca gaccccgccg
     6241 gactatgacc aggcctaccg gtacgaagac gtccgatata ccaactggtc caaaatcccc
     6301 gtcctgatgc ctgccattaa aaaggtcctc acctggatca ttggctcgga aagaactaac
     6361 cacttgtctc tcagggtctt attcttgcat gtttatgaca tctatttcta tattaccctg
     6421 atactcgaga tcgccctgct cctgggtacc atccggattc aaattcgcat ccggcgaaat
     6481 cccgcccccc tccacctgga accgagcgtt taacaccccc tgatcccgcc cctgcaccta
     6541 aatcctgctt tttaatagag ctttaatctc acattatata ctaatccgta cctcgtcttc
     6601 gttaatgatc tgttactcac tgactctgac catcttataa gaa
//
LOCUS       DASYSV010000416         5840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1616662, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000416 DASYSV010000000
VERSION     DASYSV010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616662"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1844)
                     /locus_tag="VGQ51_12795"
     CDS             complement(<1..1844)
                     /locus_tag="VGQ51_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEV9037501.1"
                     /translation="MNEATILPSFLHSPRLFQLTDRLLLSHPQRVYCRNLQGSSPAFV
                     ISAIFSQDSTSQLNHLVVCEDAEAAAYLHNTLESLTGALDLFYFPASFKNKKNYRLLN
                     SSHVMLRTEALTRLTAARGTNKKLIVTYPEALFEKVVLSSTLSGNIISIKTGDTLDLD
                     GLLEKLVDKGFERTDFVYEPGQFALRGGILDIYSFGNEKPYRVELFGNDVDSIRIFDP
                     ETQLSERKLLQVNIIPNVENRSDTGEKISLLEFVPENTVIWMEDRTFTRERLVIQEEE
                     LGLWIGHEKEVARLREESRLAEEKKAPGRHDAKRRLQQADGSMDGDEDRMEKHEVSVE
                     EFVTAVEIEGQLDKFHVVEFGASAGGHEARSGASAGFGESEVIDFHTKAQPSFNRQFE
                     LLIKDLKALESKKYTLYLFAENPRQLERLHTIFEDLKANIPFTPVALSIHEGFIDDDL
                     KIVCYTDHQIFQRYHKYKVKQAYNKNKAITLRTLRELQPGDYVTHIDHGVGVYSGLQK
                     IESGGRLQEAVRIIYKDSDILYVNINSLHKISKYTGKEGTAPKVNKLGSDAWQKLKEK
                     TKTKVKEIAFDLIKLYAQRKAEKGFAHTPDNYMQTELEASFIYEDTPD"
     gene            complement(1986..2429)
                     /locus_tag="VGQ51_12800"
     CDS             complement(1986..2429)
                     /locus_tag="VGQ51_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9037502.1"
                     /translation="MIVYLSGLPGSGWISAGGRQEQGIEGVARRIAGNHMPSPHYHPG
                     LPAGVRSTICIFRLTRQDQVIPAAGTGLYSAVKTPLVSKVETDDSGRFRILLPPGTYS
                     LFTKKGALFYATRRDEKNNIAPVEVLPGKMTRVDCSVESDHTAVF"
     gene            2487..>5840
                     /locus_tag="VGQ51_12805"
     CDS             2487..>5840
                     /locus_tag="VGQ51_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV9037503.1"
                     /translation="MNKSRIPSLYLSVFLLLFVLTGPTASSQVLTNTPVLQKTSLQLA
                     AQHLAMQKILAQKAKENGWPMILQYRHGRMAYLSHIDSRGVPVYVTTTDNIISAASIR
                     TSALWTNGSSKLNLTGGTNPNMKGRIAVWDEGLVRPTHVELTGRVLQKDGNTTLSDHS
                     THVSGTMIAAGVNPAAKGMAFGAQQLQTWDFDNDESEMAQAAAGANGILISSHSYADL
                     AGWNFNDAANRWEWWGQPNDTVDISFGLYDEDTQIWDSIAYNAPLYTIVKAGGNNPGE
                     QGPSIGATYWRRNAQHQFVNSGPRPSGISNNGGFNTLATYGNAKNIITIGAVSAIPGG
                     YSKPSDVLLAEFSSMGPTGDGRIKPDLVADGVDVLSSISTADNAYDIFSGTSMATPAC
                     AGSSFLIQEYWSRLHSGNFMRSATLRGLLIHTADEAGAFPGPDYTFGWGLIDMQAAAG
                     VITSDNTDQSQMVRESSLTNGTKDVETFIVTASGKTPIKATICWTDPAANPASIPVNE
                     HNFADTSRKLINDLDLRIKDNTTGQVYMPWILDPKNPGHAATKGDNIRDNVEKVELLD
                     SLIPGRSYTITVSHKGTLQRGTQAYSLLVSGVGGTAPCTSSSSASGASIDQVSIGLYG
                     FSSPLNSCRTSTDYTGLQTFPFAVGENTAFSITHTSCNSTNNTRVISVYIDLNNDGTF
                     GSNELVAQSAAVLSGTFTGNIAIPATATVGAYTRMRIIAEETSTPSAVLPCGSYTTGE
                     TQDFRVHFINPINDVGVTAVEYPTITTCANDSQLVSIHIHNFGSVAQTGGVPVTTVVS
                     NGPTTILTLSAICKDSIPVGGDVVYTYNKTFPTVAGVTYTFTSTTSLSTDVNTGNDAD
                     TATISVNPASTLPTGTATACGPDATSVVLHANATGDDLALWYDSQTATVPIAAGNNTS
                     TTDITPNKTYYLGLNDLKVKGGAPSKNSFTSSNPNFGGAYFRFGDNFMMFTTSVPLTI
                     ESAKMYIGHSGIIGLTLGQLASFDNNTGRYSFLPLDTVTLNVYATTPTPHDTIQWNVA
                     AGDNTDTGATFLLNIPVPNPGNYIIIFNCFNHATAFLNANIRTNPYPLSIPGVFSVTG
                     NDFRDIGKADSVTFSHQFYYPFYDIGLRLY"
BASE COUNT         1365 a         1866 c         1333 g         1276 t
ORIGIN      
        1 tgatcgggcg tgtcctcata gatgaaggag gcctccagct cggtctgcat ataattgtcg
       61 ggagtatgcg caaatccttt ttccgcctta cgctgcgcat acagcttgat cagatcgaag
      121 gctatctcct tgactttcgt cttggtcttc tccttcagct tctgccaggc gtcgctgccc
      181 agcttgttca ccttcggagc ggtaccttcc ttacccgtat atttcgatat cttatgcagc
      241 gagttgatat tgacatagag gatatcgctg tccttataga tgatccgtac cgcttcctgc
      301 agcctgcctc ccgattctat cttctgcagt ccgctgtata cgccgacgcc atgatcgata
      361 tgcgtgacat agtcgccggg ctgcagctcc cgcaacgtac gcagggtgat cgccttgttc
      421 ttgttgtagg cctgcttcac cttgtattta tggtaccgct ggaagatctg atggtcggta
      481 taacagacga tcttcaggtc gtcatcgata aaaccttcat gaatggacaa cgccaccggc
      541 gtaaaaggaa tgttcgcctt aagatcctcg aatatggtat gcaaacgttc cagctgccgc
      601 gggttctcgg caaacagata caacgtgtat ttcttgcttt ccagcgcctt aaggtccttg
      661 atcagcaact cgaactgtcg gttgaaagac ggctgcgcct tggtatgaaa atcgatcact
      721 tcactctccc caaaccccgc cgaagccccg ctccttgcct cgtgtccccc ggccgatgcc
      781 ccgaactcca ctacatgaaa tttatccagc tgcccctcta tttccaccgc cgtcacgaat
      841 tcctccacgc tcacttcatg tttctccatc cggtcctcat ccccatccat gcttccatcc
      901 gcctgctgca gcctgcgttt cgcatcatgc cgcccgggcg ccttcttttc ttcggcgagc
      961 cggctttcct cccgcagccg cgccacctct ttttcatgcc cgatccacaa cccaagctct
     1021 tcctcctgta tgaccaaccg ctcccttgta aaagtccggt cctccatcca gatcaccgta
     1081 ttctccggca caaattccag aagggatatt ttttcgccag tatccgacct gttctccaca
     1141 tttgggatga tattgacctg caatagcttg cgttcgctca attgcgtctc gggatcgaag
     1201 atcctgatag aatccacatc gttgccaaat aactcgacac gataaggctt ttcattccca
     1261 aaagaataaa tatccagtat gcccccgcga agagcgaact gcccgggctc gtacacgaag
     1321 tccgtacgct cgaaaccctt gtccaccagc ttttccaaaa gcccgtccag gtccagcgta
     1381 tcccccgttt tgatggaaat aatatttccc gaaagcgtcg aagacagcac caccttttcg
     1441 aaaagggcct cgggatacgt gacgatcagc ttcttattgg tgcccctggc cgcggtaaga
     1501 cgggtcagcg cttcggtccg caacatgacg tggctgctgt tgagcagccg gtaattcttt
     1561 ttattcttaa aggaagccgg gaaataaaaa agatccagcg cccccgtcag gctctcgagc
     1621 gtattatgga gataggcagc cgcttccgcg tcctcgcata ccacgaggtg gttcagctga
     1681 ctggtactgt cctgactgaa aatggcgctg atgacgaagg caggactgct gccctgcaga
     1741 ttccggcagt atacccgttg cggatgggac aatagaagcc tgtccgtcaa ctgaaaaaga
     1801 cggggagaat gtaaaaatga cgggaggata gtggcctcat tcatcgggcg gcaaaggtac
     1861 gaaatttcgc ccgcaaaatc cgtctctcca cgcccttccc gcccgccccc gggcaacaaa
     1921 tacaatataa ttcccctctc ttctgcccaa gcaaccgata cattaaaatt accgtaaata
     1981 cacttttaga atacggcggt atgatcggac tctacactgc aatccacccg ggtcatcttc
     2041 ccgggtagta cttctacggg cgcaatattg ttcttctcgt cccttcgggt ggcgtagaac
     2101 aaggctcctt tcttggtgaa caaagagtac gtccccggcg gtaaaaggat acggaaacga
     2161 ccggaatcat ccgtttccac cttactgacc agcggtgtct ttacagcgct gtacaagcct
     2221 gtaccggccg cgggtataac ctggtcttgc cgggtcagcc ggaatatgca aattgtagac
     2281 cggacccctg cgggtagccc gggatgatag tgcggagaag gcatatgatt tccggcaata
     2341 cgccgggcaa ctccttcgat accttgctcc tgccgacctc ccgctgagat ccatccggaa
     2401 cccggtagac ccgacagata tacaatcata ataaaggcgc gcaagtccat aaaaactgta
     2461 attttagaat gttttaataa atatttatga ataaaagcag gatcccgtcc ttataccttt
     2521 ccgtcttcct actcctattc gtactcaccg gaccgactgc cagctcacag gtgctcacca
     2581 atacaccggt acttcaaaag accagcctgc aactggccgc ccaacacctt gccatgcaga
     2641 agatattggc gcagaaggca aaggaaaatg gatggccgat gatcctgcaa tacagacacg
     2701 gccggatggc ttatctctca cacatcgact cccgcggcgt tcccgtctat gtcaccacta
     2761 cggataatat catctccgcg gccagcatcc gcaccagcgc actatggacc aatggcagct
     2821 caaagttaaa ccttaccggg ggtacgaacc ccaatatgaa aggccgtatc gcagtgtggg
     2881 atgaaggact tgtccgtccc acccacgttg agctcaccgg acgcgtgctc caaaaggatg
     2941 gaaatacaac cctcagcgat cactctacac acgtatcggg taccatgatc gctgccggcg
     3001 tcaatcccgc agcaaagggt atggccttcg gcgcacaaca gctccagacc tgggatttcg
     3061 ataacgatga atccgaaatg gcccaggccg cggcaggcgc gaacgggatc ctcatctcca
     3121 gccactccta tgccgacctc gccggttgga atttcaacga cgccgccaat cgctgggaat
     3181 ggtggggtca gcccaacgat acagtcgaca tcagcttcgg cctctacgat gaggacaccc
     3241 agatatggga ctccatcgct tataacgctc ccctctatac catcgttaag gcaggtggta
     3301 ataaccccgg cgaacagggc ccttccatcg gagccaccta ctggcgcagg aatgcccagc
     3361 accagttcgt caattcaggc ccccggccct ccggtatcag caataacgga ggcttcaata
     3421 ctctcgccac ctatggcaat gcaaagaaca tcatcactat cggggccgtc agtgcgatcc
     3481 ccggcggcta ttccaaaccg tctgatgtgt tgctggcaga gttcagcagt atggggccca
     3541 ccggtgatgg ccgcatcaaa cccgatctcg tcgccgacgg cgtcgatgtc ctttcgtcca
     3601 tctccacggc cgacaatgcc tatgatattt tcagcgggac ttcgatggcg acacccgcat
     3661 gcgccggctc ctcctttctc atccaggaat actggtcgag actgcatagc ggcaatttca
     3721 tgcgaagcgc aacccttcgc ggtctgctca tccatacggc cgacgaggcc ggcgcctttc
     3781 ccggtcccga ctatactttc ggttggggac tgatcgatat gcaggcagcc gcgggcgtca
     3841 tcacctccga caacaccgac cagagccaga tggtccgtga aagctcgctc accaatggca
     3901 caaaagatgt cgagacattc atagtgactg cttccggcaa aacccccata aaggctacca
     3961 tttgctggac tgatcccgcc gccaacccgg ccagcatccc cgtcaacgaa cataacttcg
     4021 ctgacacctc ccgcaaactc atcaatgacc tcgatctccg cattaaggat aatactaccg
     4081 gccaggtata catgccctgg atactcgatc cgaaaaatcc cgggcacgcc gccactaaag
     4141 gcgacaacat tcgcgacaac gtagaaaagg tcgagctgtt ggacagcctc atccccggca
     4201 ggtcatatac cattactgtc agccataaag gcaccctgca acgcggcacc caggcctatt
     4261 ccctgctcgt cagcggggtc ggtggtactg ccccttgcac cagttcatcc agcgccagcg
     4321 gcgccagcat cgaccaggtg tctatcggtc tctatggatt ctccagcccg ctcaacagct
     4381 gcaggacctc tacagactac accggcttgc agacattccc tttcgccgtc ggcgagaaca
     4441 ccgcattcag catcacacat accagctgca acagcaccaa caatacccgc gtcatttctg
     4501 tctatatcga tctcaacaac gacgggacct tcggatccaa cgaactggtg gcccaaagcg
     4561 cagccgtcct ttcgggcacc ttcacgggta acatcgccat tccggctacg gccaccgtgg
     4621 gcgcctatac acgcatgcgc atcatcgcgg aggaaacgag caccccttcc gccgtcctgc
     4681 cctgcggcag ctacacgaca ggtgagacgc aggacttccg cgttcacttt atcaatccca
     4741 tcaacgacgt cggcgtcact gcggtggagt atcccaccat caccacttgc gccaacgatt
     4801 cacagctggt atctatacat atccataact tcggctcggt agcgcagacc gggggagtgc
     4861 cggtcaccac ggtcgtcagc aacggaccga ccaccatact cactctgtcc gccatctgca
     4921 aagacagcat ccccgtcgga ggcgatgtcg tatacaccta taataaaacc ttccctacag
     4981 tagcaggcgt cacctatacc tttacgagca cgacatcgct ctccacggat gtgaacaccg
     5041 gaaacgacgc ggacaccgca accataagcg tcaatcccgc ttcgaccctc ccgacaggca
     5101 cagccaccgc gtgcggccct gatgcgactt ccgtagtact gcatgccaat gcgaccggag
     5161 acgatctcgc cttatggtat gacagccaga cagccaccgt acccatcgca gccgggaata
     5221 atacctcgac caccgatatc acccccaata aaacgtacta tctcggtctc aacgacctca
     5281 aggtaaaagg cggggcgccg agtaaaaact ccttcacgag ctccaacccc aacttcggcg
     5341 gcgcctattt ccgtttcggc gacaatttca tgatgttcac taccagcgtg ccactcacca
     5401 tcgagagcgc caaaatgtat atcggccact cgggtatcat cgggctcacc ctcggccagc
     5461 tggccagctt cgataataac accgggcgat attcctttct cccgctggat accgtaacac
     5521 tcaatgtcta cgctactaca cctactccgc atgatactat ccaatggaac gttgccgccg
     5581 gcgacaacac cgataccggg gccaccttcc tgctgaacat ccccgttccc aaccccggca
     5641 attatatcat catcttcaat tgcttcaacc acgcgacggc cttcctcaac gccaatatca
     5701 ggaccaaccc atatccctta tccatacccg gggtcttctc ggtaacgggc aacgacttcc
     5761 gcgatattgg caaggcggat agcgtcacgt tctcacacca gttctattac cccttctatg
     5821 atatcggtct gcggctctac
//
LOCUS       DASYSV010000417         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_596895, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000417 DASYSV010000000
VERSION     DASYSV010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596895"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VGQ51_12810"
     CDS             complement(<1..864)
                     /locus_tag="VGQ51_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037504.1"
                     /translation="MNRITLFALFLLSAVLASGQQLQLELPMDSTGVEGLRISGITYS
                     ATGNVLLSLASQNNVRQYRLSGDSTAGAPVCLASIDYDGKKSHYLVAAGLPDGIETCI
                     SNKDYSILRFLHTGRPDRTDSLVIPSGQQLFYAFYQGNTFYAIAVQKHSNTLFVYRRT
                     PQAPTQTFQKEIDIGKWGIIWWHGTRAFAKNAVDNCYDLMRSVGMTQEDSNTPPPMSS
                     VVFKSKCYIREGMLYLTFDTHQLVTKVIEVPLDGRPGRLLQFNPEDWYHKSLPIGFST
                     GNSFIFDNTLIT"
     gene            complement(868..1782)
                     /gene="accD"
                     /locus_tag="VGQ51_12815"
     CDS             complement(868..1782)
                     /gene="accD"
                     /locus_tag="VGQ51_12815"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEV9037505.1"
                     /translation="MAKHEEDFDANLTGDEGQSEESKSGWFRRIKKGILTSTAEKKET
                     PEGLWTKCPNCNYICTVTELRESLFVCPKCNHHHRIGSTEYFEILFNDNHYEVLFENI
                     RSKDFLHFTDLKSYQKRLEETWAKTEITDSIRIATGEVKEHRMVIGCMDFEFIGGSLG
                     SVMGERISCAADYCLSHRIPLLIICKSGGARMMESAFSLMQLAKVSGKLSQLTDAKIP
                     YISLLTDPSFGGISASFGMLGDLNIAEPGALIGFAGPRVIKETIKKDLPEGFQRSEFL
                     LEHGFLDFIVNRKELKERIATLMTLFKG"
     gene            1950..2633
                     /locus_tag="VGQ51_12820"
     CDS             1950..2633
                     /locus_tag="VGQ51_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEV9037506.1"
                     /translation="MKWSELFTSSVGKKFVMGLTGIFLILFLIVHVCVNACIWAMDDG
                     QMFNLAANFLGATVVPRVLEIGLFIGFILHIVQAYVLENQNRKRRGVGYQVPMGNKGS
                     KWYSRYMGWLGTFVLLFLVIHLKDFWVPSRFQEMPEATYDPGIRVHDLFSLMKDTFQN
                     GWIVIIYLVGCLALAYHLMHGFQSAFRSLGVYNSRYLRLINGIGIAFSVIVPLAFAMM
                     PVSMYFNWV"
     gene            2663..>3563
                     /locus_tag="VGQ51_12825"
     CDS             2663..>3563
                     /locus_tag="VGQ51_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV9037507.1"
                     /translation="MLNSKIPAGQLDKKWEDYKGHVRLVNPANKRHLEVIVIGTGLAG
                     ASAAASLGEMGYKVKAFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSTFRLFYDTIKG
                     GDYRSREANVYRLAEVSASIIDQCVAQGVPFAREYGGLLSNRSFGGVQVQRTFYAAGQ
                     TGQQLLIGAYQALERQVALGNVEMYSRHEMLDVVVIDGKARGIIARNLITGQLERYFG
                     HAVLLCSGGYGNVFYLSTNAMGSNVTAAWKAHKKGAWFGNPCFTQIHPTCIPVTGEHQ
                     SKLTLMSESLRNDGRIWVPKQKND"
BASE COUNT          770 a          909 c         1007 g          877 t
ORIGIN      
        1 cgttatgagc gtgttatcga agatgaacga attgccggtg ctgaacccga taggcagtga
       61 tttatgatac cagtcttcgg ggttgaactg cagcagccgg ccggggcgac cgtcgagggg
      121 tacttcgata acttttgtca cgagctgatg ggtgtcgaag gtcaggtaga gcattccctc
      181 ccggatatag cacttgcttt tgaagaccac ggagctcatg ggtgggggag tgttgctgtc
      241 ctcctgtgtc attcctacgc tccgcatcag atcgtaacag ttgtcgacgg cattcttcgc
      301 aaaggctctc gtgccgtgcc accatatgat accccatttc ccgatatcga tctctttttg
      361 aaatgtctgg gtgggggctt gtggtgtccg ccggtagacg aagagggtat tggagtgttt
      421 ttgaacggcg atcgcataga aagtgtttcc ctgatagaac gcgtaaaaga gttgctggcc
      481 agacgggatc actagcgaat ccgtgcgatc cggacgtccg gtatgcagga accggaggat
      541 ggaataatcc ttgttggaga tgcaggtttc gattccgtcc ggcaatccgg ccgcgaccag
      601 gtaatggctt tttttgccgt cataatcgat agaggcgaga caaaccgggg cgccggcagt
      661 gctgtctccg gaaagacgat attgtcggac gttgttttgc gaagccagtg agagaaggac
      721 attccccgta gcgctatagg tgatgcccga gatgcggaga ccctccaccc cggtggagtc
      781 cataggcaat tccaattgca gctgctggcc cgatgcgagg acagccgaca gcaggaacag
      841 ggcaaaaagg gtgattctat tcacgggtta gcctttgaac aacgtcatca gcgtggcaat
      901 gcgctccttg agctcctttc tattaacgat aaaatcgaga aaaccgtgtt cgagaaggaa
      961 ttcgctgcgt tggaaacctt cggggaggtc ttttttaatg gtttctttga tgacgcgggg
     1021 gccggcgaaa ccgatcaggg cgccgggttc tgcgatattc aagtcgccga gcatgccgaa
     1081 tgaggcggaa atgcccccga atgaagggtc ggttaacagc gagatatagg gtattttggc
     1141 gtccgtcagc tgtgagagct tgcccgaaac cttggccagc tgcatcagcg agaaggcgct
     1201 ttccatcata cgggctcccc cggatttgca aatgatcagc aggggtatcc ggtggctgag
     1261 acaatagtcg gccgcacatg agatccgctc gcccatgacc gagcccaatg atccgccgat
     1321 gaattcgaaa tccatacacc cgatgaccat ccggtgctcc ttcacctcac ccgtggcgat
     1381 gcggatggag tccgttatct cggtcttggc ccacgtttcc tccagtcttt tctggtagga
     1441 tttcagatcg gtgaaatgca ggaaatcctt ggaacggatg ttctcgaaca ggacttcgta
     1501 gtggttgtcg ttaaagagta tctcgaaata ttcggtgctg ccgatacggt gatggtgatt
     1561 gcatttcggg catacgaaga ggctttcccg gagttcggtc accgtacaga tatagttgca
     1621 attggggcat tttgtccaga gaccctcggg ggtctccttt ttttccgccg tggaggtaag
     1681 gatccctttt ttaatgcgtc tgaaccatcc gctcttgctc tcctcgcttt gtccttcgtc
     1741 accggtcagg tttgcatcaa aatcttcttc gtgctttgcc atttgaggta atttgttcag
     1801 atcagcggct aagatactaa acatccgcga tttgtttggc aactgttcgt gtgctaaatt
     1861 tgcgcggtcg acccaaaacg ggtccccgca caattatgac acgagccgca gcgactgcat
     1921 gcggccattg aaaagacaaa tatgatcgca tgaaatggtc tgaactcttt acttcctctg
     1981 tcggcaagaa attcgtgatg ggtctcaccg gcatttttct gatccttttc ctcatcgtcc
     2041 acgtatgcgt gaatgcctgc atttgggcga tggatgacgg ccagatgttc aacctggccg
     2101 ccaattttct cggggcgacc gtcgttccgc gggtgctgga gatcggcctg ttcatcggat
     2161 ttatcctgca catcgtgcag gcctacgtcc tggagaatca aaaccggaaa aggaggggcg
     2221 tcggctacca ggtacccatg ggtaataagg gtagcaaatg gtattcccgt tatatgggct
     2281 ggctgggcac attcgtcctg cttttcctgg tcatccatct caaggacttc tgggtcccgt
     2341 cccggttcca ggaaatgccg gaagctacct atgaccccgg tatcagggtg catgaccttt
     2401 tcagcctgat gaaggacacg tttcaaaacg gctggatcgt cattatctac ctggtgggtt
     2461 gtctggcgct ggcctatcat cttatgcatg gtttccagag tgcgtttcgc tcgctgggcg
     2521 tatataacag ccgatatctc cggctgatca acggtatcgg tatcgcgttt tccgtcatcg
     2581 tgccgctggc tttcgccatg atgcctgtct ccatgtactt taactgggtt tgatcatcct
     2641 tgaaataatc aaaaaattac tcatgttgaa ttcaaagatc cccgcagggc agctggacaa
     2701 aaaatgggag gactataaag gtcatgtcag gctggtgaat ccggccaaca agcgtcacct
     2761 cgaagtgatc gttatcggga cgggtctggc cggcgcctcg gcagccgctt ctctcggtga
     2821 gatggggtac aaagtgaaag ccttctgttt ccaggattcc ccgcgccggg cgcatagcat
     2881 cgcggcccag gggggcatca atgccgctaa gaattatcag aatgacgggg acagcacctt
     2941 ccgtcttttc tatgatacca ttaaaggcgg agactaccgt tcgcgcgagg ccaatgtcta
     3001 tcgcctggcg gaagtaagcg ccagtatcat cgatcaatgc gtcgcgcagg gagtgccttt
     3061 cgcccgcgaa tacggaggtc tcctgagcaa ccggtcattt ggcggcgtac aggtccagcg
     3121 gactttttat gctgccgggc agacaggcca gcagttgctg atcggcgcct accaggcctt
     3181 agagcgccag gtggcgcttg gtaacgttga aatgtacagc cggcatgaga tgctggatgt
     3241 ggtggtcatc gacggcaagg cccgcggcat catcgcgcgc aatctcatta ccgggcagct
     3301 tgaacgctat tttggtcatg ccgtccttct ttgcagcggc ggctatggca acgtttttta
     3361 cctgtcgacc aacgccatgg gcagcaatgt cacggcggca tggaaggcgc acaaaaaagg
     3421 cgcatggttc ggtaacccct gtttcacgca gatacatccc acctgtatac ccgtcaccgg
     3481 cgagcatcag tccaaactga cccttatgtc ggagtcgttg cggaatgacg gtcgcatctg
     3541 ggtgcccaaa caaaagaacg acc
//
LOCUS       DASYSV010000418         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1110177, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000418 DASYSV010000000
VERSION     DASYSV010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110177"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VGQ51_12830"
     CDS             <1..751
                     /locus_tag="VGQ51_12830"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9037508.1"
                     /translation="PAVPKTEVLANANVPKIAADSISFLNTRVASLDFKRLPANRMVY
                     AGQVLDFNNRPLAGASLLVSGPSNAVTTTNALGQFKLRLRPQDTIQRLTVVMSGYMDA
                     SLTVNQLNSDQVTGNTIVLRERPAALNEVVVSGADRRAKEAFATAPFEDKTEKLDSFW
                     VRVTPVTGRLAYLDYLSAAKKTLPVDTTIRGAEVLSFAIDPKGTPTDFKIEHSLSPAH
                     DAGVIRLITDGARWKIVRGKNIRAMVSVSFP"
     gene            784..1617
                     /locus_tag="VGQ51_12835"
     CDS             784..1617
                     /locus_tag="VGQ51_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEV9037509.1"
                     /translation="MKKHLFLLAFAACLYLPSIAQQIKTPAPSAPQFVRQDFGLSSIE
                     LSYSRPDMRGRKIFGDLVPYGKVWRTGANQATTLTFGDPVTIGGTKIPAGKYGLLTIP
                     GADDWTLIITHQTDVTSPAAYKPDQDIVRVTAKPYQLPFPMETFTILFANVTANACDL
                     QLIWENTVVALPITTDIDSHIMAQIDNAMNKDNHPYYSAAFYYLENGKDLNKALEWFD
                     KAIAQDPTAFFVVYQKARCLAKMGRKAEAKTTAEKGIELAKQANNADYVALNQKLISS
                     L"
     gene            complement(1609..3333)
                     /locus_tag="VGQ51_12840"
     CDS             complement(1609..3333)
                     /locus_tag="VGQ51_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV9037510.1"
                     /translation="MSRPDWIVLALTLSGIIAYGLYKGRATKDLDGYFLSNRTVPWYM
                     VMLGIMGTQASAVTFLSAPGQAYTDGMRFVQYYFGLPLAMVVVCVSFVPVFCRLKVYT
                     AYEFLEQRFDLKTRTLTSFLFLLQRGMSTGISIAAPSIILSSLLGWDLLWTTIFMGGL
                     LIVYTVSGGARAVAYTQQLQMIVIFSGLLIAAYVVVHKLPSGVGMSEILHIGGRAGKM
                     NIITTGFDKGHHGFDWNDRYNLLSGIIGGFFLALSYFGTDQSQVGRYLTARSVGESRL
                     GLLLNGLVKVPMQFGILLLGVMVFAFYHFNAEPVFFNKSQLTLLSHTKYKDSLDIVTR
                     RYDALERERVALVSAHPDGDVVAIEREKAGIKATIRRWLDDKSVGGDSNDTNYIFLRF
                     VTDYLPAGLVGLLIAVIFLAAWGSIAATLNSLASCTIVDFHRRYARVALSTRKEYRLS
                     QLYTLFWGIFCVAVAQLTYGLGNSLIEAVNLLGSLFYGVILGVFLVAFYRKRVGSHAV
                     FISAIVTEIGVIVLNMLSRKGIVPLGFLWYNLVGAAGVVLLSEVLQALIGKKKIAGPG
                     PGYPTGSE"
     gene            complement(3330..3461)
                     /locus_tag="VGQ51_12845"
     CDS             complement(3330..3461)
                     /locus_tag="VGQ51_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037511.1"
                     /translation="MDTDNKDGGRAPLFGKWVYWYILVIGFLLFCILVFDFLTKHFS"
BASE COUNT          899 a         1148 c          816 g          665 t
ORIGIN      
        1 acctgccgtc cccaaaaccg aagtcctcgc aaacgccaat gtcccaaaga tcgccgccga
       61 cagcatctct ttcctgaaca caagagtcgc cagcctcgat ttcaagcgcc tgccagccaa
      121 ccgcatggta tacgcaggtc aggtcctcga cttcaacaac cgcccccttg ccggcgcctc
      181 actgctggtc tccggtccct ccaacgccgt cacgacgacc aatgcccttg gccagttcaa
      241 actcagactc cggccgcagg ataccataca acggctgacc gtagtcatgt ccggctacat
      301 ggacgcttct ctcacggtca atcagctcaa ttccgatcag gtgacaggca ataccatcgt
      361 cctccgggaa cgacccgcgg cactcaacga ggtagtggtc tccggtgcgg accgccgggc
      421 aaaagaagct ttcgccaccg cgcctttcga agacaagact gaaaaactgg attctttttg
      481 ggtcagggtc accccggtca ccggccgtct ggcctatctg gattatctgt cggcagccaa
      541 aaaaaccctc cccgtcgaca ccaccatccg gggcgccgag gtcctctcat tcgccatcga
      601 tccgaaaggg acacccaccg attttaagat cgaacattcg ctctcccctg cccacgatgc
      661 cggcgtcatc cggctcatca ccgacggagc ccgctggaaa atcgtccgtg gcaaaaacat
      721 ccgggccatg gtcagtgttt ccttcccata aagccgtaat ttcagccctc aaaatcatca
      781 cacatgaaga agcatctatt cttgctcgct tttgcagcct gcttgtacct cccttccata
      841 gcccaacaga taaaaacccc ggccccttcg gccccccaat tcgtcagaca ggatttcggt
      901 ctctcctcga tcgaactttc ctattcccga ccggatatgc gcggccgtaa gatattcggc
      961 gacctcgtgc cctacggcaa ggtttggcgc accggtgcca accaggccac taccctcacc
     1021 ttcggtgacc ccgtgaccat cggcggcacg aagatacccg ccggcaaata cggtctgctc
     1081 accatccccg gtgccgacga ttggaccctc atcatcacac atcagacgga cgtcaccagc
     1141 cccgccgctt acaaacccga ccaggatatc gtacgcgtga cggccaaacc ctaccagctg
     1201 ccctttccaa tggaaacatt cacgatcctt ttcgccaacg ttacggccaa cgcctgcgat
     1261 ctccagctca tctgggaaaa tacggtggtg gcgctgccga ttacgacaga catcgattcg
     1321 catattatgg cgcagatcga caatgccatg aataaggaca accatcctta ttatagcgcc
     1381 gccttttatt acctcgagaa tgggaaagat ttgaacaagg ccctcgaatg gttcgacaag
     1441 gccatcgcac aggacccgac cgccttcttc gtcgtctacc agaaagcccg ctgtctcgcc
     1501 aaaatgggca gaaaggccga agccaaaacc actgcggaaa aaggcatcga actggcaaaa
     1561 caagccaaca atgccgacta tgtggccctc aaccaaaaac tcatttcttc actctgatcc
     1621 cgtcggatag ccgggtcccg gcccggctat cttttttttc cctatcaggg cctgcaacac
     1681 ctcactcaac aataccactc ccgcggcgcc caccaggttg taccacaaaa aacccagggg
     1741 aacgatcccc ttccggctca gcatattcag cacgatgacg cctatctcgg tcacgatggc
     1801 actgatgaat accgcatggc ttcctacccg cttgcgataa aaagccacga ggaacacacc
     1861 caggatcacg ccataaaaaa gagagcccaa caggttcacc gcttcgatca ggctgttgcc
     1921 caatccatat gtcaactgcg cgacggcgac gcaaaagatc ccccagaaca acgtatacag
     1981 ctgactaagc cggtattcct tccgggtcga caaggcgaca cgtgcataac gacgatggaa
     2041 gtccacgatg gtacaggagg ccagcgagtt caacgtcgcg gcgatcgaac cccaggctgc
     2101 cagaaagatc acggcgatca gtaatcccac cagccccgcg ggcagatagt ccgtcacaaa
     2161 acgcaaaaag atatagttcg tgtcattcga gtcgccgccc acgctcttgt catccagcca
     2221 gcgcctgatc gtcgccttga tgccagcctt ctctcgctcg atcgcgacaa catccccatc
     2281 cgggtgcgca ctcaccagcg ccacacgttc ccgctccaac gcgtcatacc gccgggtcac
     2341 gatgtcgagc gagtccttat atttcgtatg tgacagcagt gtcagctgcg atttattgaa
     2401 aaagacaggc tccgcgttaa aatgataaaa agcgaatacc atgaccccca gcaacaggat
     2461 accgaattgc atcggcactt tcacgagccc gttcagcaat aagccgagcc ggctttcacc
     2521 gacagacctc gcggtaagat aacgccctac ctgggattga tcggtaccga aataagacag
     2581 ggccagaaag aatccgccga tgatcccgct caacaggttg taccggtcgt tccagtcgaa
     2641 cccgtgatgc cccttgtcga agcccgtcgt gatgatgttc attttccccg cccgtccgcc
     2701 gatatgcagg atctccgaca tcccgacacc cgatggcaac ttgtgcacga cgacatacgc
     2761 ggcgatcagc aacccggaaa aaatgacgat catctgcagc tgctgcgtat atgccacggc
     2821 ccgggcccct cccgataccg tatagacgat gagcaggcct cccatgaaga tagtggtcca
     2881 tagcagatcc caacccaaca gcgatgagag gatgatggaa ggagcagcga tgctgatccc
     2941 ggtggacatc ccgcgttgca gaagaaagag gaaagacgtc agcgtccgtg tcttgagatc
     3001 gaaacgctgc tccaggaatt catacgccgt atagaccttc aacctgcaaa agaccgggac
     3061 gaagctgacg caaactacca ccatcgccaa cggcagaccg aaataatatt gcacgaaccg
     3121 catgccgtcg gtatatgcct gacccggcgc ggaaaggaag gtgaccgcac tggcctgcgt
     3181 acccatgatg cccagcatca ccatatacca gggaacggtc cgattggaaa ggaaataacc
     3241 gtccaggtcc ttcgtggcac ggcccttgta caatccatag gcaatgatcc ccgatagcgt
     3301 cagcgccagt actatccagt caggccggct catgaaaaat gtttggtcag aaaatcgaat
     3361 acaaggatac agaacaggag gaacccgatc accagtatat accagtaaac ccatttccca
     3421 aataaaggag cccgtccccc gtccttgtta tccgtgtcca tattatttcc gattgcggtt
     3481 ggccatcagc gatgcgacaa gcaacccgat agccagcccg atcaataa
//
LOCUS       DASYSV010000419         4766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_468424, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000419 DASYSV010000000
VERSION     DASYSV010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468424"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VGQ51_12850"
     CDS             <1..199
                     /locus_tag="VGQ51_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037512.1"
                     /translation="KQLRDMGATIGIGANSLKSPLSYQIIFQYGIKGTSQNNIIRENY
                     MNLTFVLNFGAIWYTKGRKFD"
     gene            206..952
                     /locus_tag="VGQ51_12855"
     CDS             206..952
                     /locus_tag="VGQ51_12855"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9037513.1"
                     /translation="MLDLHQQMKGFPMMGVLNCIVVEDNNKQSALLKDYIAKTPNLSF
                     SGKCESAADVYSLVIQHRADIIFWDIRLLDNRVMLRLRESGYYPIVICVADKQEQEDK
                     ETETDVFSYLIRPLSFERFLSTMNKIRDYLSTTIYLHHRNNRFVFIKSEYKIIKVRFE
                     DILFCEGMKDYTQIYVRGRAEPILTLSNLKSFSYKLPPDEFIRVHRSFIVSLNHIDSI
                     ARNEIYIGKKIIPIGDSFKDDFYQIIEWNS"
     gene            962..1795
                     /locus_tag="VGQ51_12860"
     CDS             962..1795
                     /locus_tag="VGQ51_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HEV9037514.1"
                     /translation="MIKIAILDLYEGQPNQGMRCIRALLTEYAHTHSLALTFDEFEVR
                     LRHETPDLSYDIYISSGGPGSPLDSEGGEWEAHYWDWLKKIENFNNDPGEAAPKQILF
                     ICHSFQLACRYYGIARVTSRKSTAFGVFPVHMLEGAGSEPIFNGLRDPFYAVDSRSYQ
                     VIEPDQDRLAHMGAQLLAIEKERPHVPLERAIMAIRFSDYMVGTQFHPEADPTGMSIY
                     LQQDDRKHTVIAEHGEEKWKSMIEQLNDPDKILWTYSHVIPNFLDYALNAAPSILPKR
                     M"
     gene            1817..3019
                     /locus_tag="VGQ51_12865"
     CDS             1817..3019
                     /locus_tag="VGQ51_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037515.1"
                     /translation="MVPALRKAFNAAFTRQRYERFLADLNSRYPGAIEFRIAETPVFV
                     PASFTARLLEACESIVDVILDPAFLKWTERAIPDNDRVPNENAHPHFLVFDFGVCEDG
                     NGGWQPQLIEMQGFASLYGFQVYYPETLRRHFDIPENYSQFLNGYDHQSYLDLLHDVI
                     VGDCTPEQVILLELKPHQQKTRIDFYCTQDYLGITPVCLTELIQEGRQLFYLHPATGK
                     KTHIRRIYNRVIFDELHNAGVMSGGNNVVDIRQDFDVEWVTHPNWFYRVSKYTLPFLR
                     HPSVPPTYFLNQLKQPPADLENYVLKPLFSFAGQGVIIDPKPADLDVKDPENWILQRK
                     VKYAGLIPTPDIPAMTEIRIMYLWKDGAPRPVPAINLARLSKGRMIGVNFNKDKEWVG
                     GSVGFFER"
     gene            complement(3020..3388)
                     /locus_tag="VGQ51_12870"
     CDS             complement(3020..3388)
                     /locus_tag="VGQ51_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037516.1"
                     /translation="MDKVLQILIVFGLILLFVYRMIRGREQIYRPYEFIIRLRAPLPA
                     EVELRMVLAEDNRLETDTLFFDYEVEGRLTIYRRRQQARGRQVQELAVPVDCTAVAMD
                     PVENKLYFEAGGYWFVYGQA"
     gene            3437..4225
                     /locus_tag="VGQ51_12875"
     CDS             3437..4225
                     /locus_tag="VGQ51_12875"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV9037517.1"
                     /translation="MDLQLKGKTALVLAASKGLGKACAAALLNEGADVIIGARNRETL
                     ETTANELRAFGKGTVLAIPVDVQDARQASEIVQAAASAFGKIDILVNNAGGPPFGKFE
                     SFDDTAWQSAFDITLLSAVRFSRLVIPHMRPTGSGRIINIVSLTVKTFMPNSVLSTSL
                     RMGVVGMAKMLSHELAPDNITVNNVAPGLILTDRIRDTLPPVSPGGPSFEQMLEQRAK
                     DIPLGRVGKPEEFAAVVTFLASPLASYITGATIPVDGGAIMATT"
     gene            complement(4222..>4766)
                     /locus_tag="VGQ51_12880"
     CDS             complement(4222..>4766)
                     /locus_tag="VGQ51_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037518.1"
                     /translation="FFFATVAGASAQTSVSPFVTAIDSILRDFPNNLRHITGDLVMAQ
                     GEFENYASMVQLPGSADCIITRWHSTGDTTASWQAKMYSGGDFNAAEKCYHQLYRQLK
                     GCYMRLQDSSLVYLSGEWNPAKEEAAFTSSTLRLGIDDWRYSKVEVELELVYQLADWA
                     VQINIVSKQPDEMPAAASRF"
BASE COUNT         1177 a         1512 c         1100 g          977 t
ORIGIN      
        1 caaacagctc agagacatgg gcgctacgat cggcatcggc gccaactcgc tcaaatcccc
       61 cctgtcctac cagatcatct ttcaatacgg gatcaaaggc acctcccaga ataacattat
      121 ccgcgaaaac tacatgaacc tgacgtttgt tctcaacttc ggcgccattt ggtataccaa
      181 aggcagaaaa tttgactaaa taattatgct cgatctccac cagcagatga aaggcttccc
      241 catgatggga gtactcaact gcatcgtcgt ggaagataat aacaagcaaa gcgccctgct
      301 caaggactat atcgccaaga ctcccaacct ttccttttcc ggtaaatgcg agtcggctgc
      361 agacgtctat tccctcgtca tccagcaccg cgcggatatc atcttctggg acatccgact
      421 cctcgacaac cgcgtcatgc tccgacttcg tgaatcgggc tattacccca tcgtcatctg
      481 cgtcgccgat aaacaggagc aggaagataa agagacggaa acggacgtat tcagctatct
      541 gatccggccg ctgtccttcg aacgttttct atcgacgatg aacaagatca gggactatct
      601 ctccaccacc atctatctcc accaccgcaa taaccgcttc gtcttcatca aatcggaata
      661 caagatcatc aaggtccggt tcgaagacat cctgttctgc gaaggcatga aggactatac
      721 gcagatctat gtcaggggaa gggccgaacc catcctgacc ctcagcaatc tcaaatcatt
      781 ctcgtataag ctcccccccg atgaattcat ccgcgtacac cgcagcttca tcgtctcgct
      841 caaccatatc gattccatcg cccgcaatga gatctatatc ggtaaaaaga tcatccccat
      901 cggagacagc ttcaaggacg acttctacca gatcatcgaa tggaattcgt aattttgtcc
      961 catgatcaaa atcgccattc ttgatctcta tgaaggacaa cccaaccagg gcatgcgctg
     1021 tatccgcgcg ctgctcacgg aatacgccca tacccactcc ctcgccctga cattcgacga
     1081 atttgaagta cgtctccggc acgagacccc cgatctctct tacgatatct atatctccag
     1141 cggagggcca ggcagtccgc tcgatagcga aggcggcgaa tgggaggccc actactggga
     1201 ttggcttaaa aaaatagaga acttcaacaa cgatcccggc gaagccgccc ctaaacagat
     1261 cctcttcatc tgccactcct tccaactcgc atgccgttac tacggcatcg cccgcgtcac
     1321 ctcgcgcaaa tccaccgcat tcggcgtatt ccccgtacac atgcttgaag gagccggatc
     1381 ggaacctata ttcaatgggc tccgcgaccc cttctacgca gtggacagcc gcagctatca
     1441 ggtcatcgag cctgatcagg accgactcgc ccatatgggc gcgcaactgc tcgccatcga
     1501 aaaggagcga ccgcacgtcc cgctcgaaag agccatcatg gccatccgct tttcagacta
     1561 tatggtcggt acccagtttc accccgaggc cgatcccacc ggcatgagca tttatctgca
     1621 gcaagacgac aggaagcata ccgtcatcgc cgaacacggc gaagaaaaat ggaagagcat
     1681 gatcgagcag ctcaacgatc ccgataagat actctggacc tactcccatg tgatccccaa
     1741 tttcctcgac tatgcgctga acgccgcccc ttccattctc cctaaaagaa tgtaactttc
     1801 gggaaaagac gccgatatgg tcccagcact gcggaaagcc ttcaacgccg ccttcacccg
     1861 gcaacgctac gaacgattcc tggccgacct caatagccgc tatcccggcg ccatagaatt
     1921 ccggatagcc gaaacacccg tcttcgtccc ggcctccttc accgccaggc tactggaggc
     1981 atgcgagtcc atcgtcgacg tcatcctcga tcccgccttt ttgaagtgga ccgaacgcgc
     2041 cattcccgac aacgatcgcg tacccaacga aaatgctcac ccgcacttcc tggtattcga
     2101 tttcggcgtg tgcgaagacg gcaacggcgg ctggcaaccc caactcatag agatgcaggg
     2161 tttcgcaagc ctctatggct tccaggtcta ctatcccgaa acactgcggc ggcatttcga
     2221 tatacccgaa aactatagcc agttcctcaa cggctatgac caccaatcct atctggatct
     2281 cctgcacgat gtcatcgtag gcgactgcac gcccgaacag gtgatcctgc tcgagctcaa
     2341 accccaccag caaaaaacaa ggatcgactt ttattgcaca caagactatc tcggcataac
     2401 accggtatgc ctgaccgaac tcatccagga aggccgtcag cttttctatc tgcacccggc
     2461 gacaggaaaa aagacgcata tccgccggat ctataaccgc gtcatcttcg atgagctgca
     2521 caacgccggc gtcatgtccg gcggtaataa tgtcgtggac atccgccagg acttcgacgt
     2581 ggaatgggtg acgcatccca actggttcta ccgcgtcagc aaatacaccc tccccttcct
     2641 ccgccatccc agtgtccctc ctacctattt cctcaaccag ctgaaacaac cgccggccga
     2701 tctcgaaaac tacgtgctca aaccactatt ctctttcgct ggtcagggcg tgatcatcga
     2761 tcccaaaccc gccgatctgg atgttaaaga cccggaaaac tggatcctcc agcgtaaagt
     2821 aaaatatgcc ggcctgatcc cgaccccgga catccctgcc atgacggaaa taaggatcat
     2881 gtatctctgg aaggacggcg cgccccgccc ggtacccgcc atcaacctcg cccgtcttag
     2941 caaagggagg atgatcggcg ttaacttcaa caaagacaaa gaatgggtgg gcggcagcgt
     3001 gggtttcttc gaaagatgat caggcctggc catagacgaa ccaatacccg cccgcttcaa
     3061 aatagagttt gttttccaca gggtccatcg ccacggccgt gcaatcgacc ggtacggcca
     3121 gttcctgcac ctgtctcccc ctggcctgct gacgccgccg atagatcgtc agacgtccct
     3181 ccacctcata gtcgaaaaac agggtatccg tttccagccg attatcctca gcaaggacca
     3241 ttcgcagttc cacctccgcc ggcagaggcg ccctcagccg aatgataaat tcatacgggc
     3301 gatatatctg ctcgcgtccg cggatcatcc ggtagacgaa cagtaagatc aagccgaaaa
     3361 cgatcaatat ctgcaatact ttatccatgc gccggcagca agataccccg tatctttagt
     3421 atctcaatcg gaaactatgg acctgcaact caaaggaaaa acggccctcg tcctggccgc
     3481 aagcaaaggc ctcggtaaag cctgcgccgc cgctctcctc aacgaaggag ccgacgtcat
     3541 catcggagcc cgcaaccgcg aaactctcga aaccactgca aacgagctcc gcgcgtttgg
     3601 caagggtacc gtcctggcca tccccgtgga tgtacaggat gcccgtcagg cttcggagat
     3661 cgtacaggcc gcagccagcg cctttggaaa gatcgatatc ctggtgaaca acgccggcgg
     3721 tccgcccttc ggaaaattcg agtcgttcga cgataccgcg tggcagtcgg ccttcgatat
     3781 tacactgctc agcgccgtcc gcttcagccg gctcgtcatc ccccatatgc gccccaccgg
     3841 cagcggacgt atcatcaata tcgtcagcct gaccgtgaag acctttatgc cgaattcagt
     3901 gctgtccacc agtctgcgca tgggagtggt gggaatggcg aaaatgcttt cgcacgagct
     3961 ggcgccggat aatattacag tcaataatgt cgcacccggt ctcatcctca ccgacaggat
     4021 cagggatacc cttccgcctg tcagccccgg cggacccagc ttcgaacaaa tgcttgaaca
     4081 gcgcgctaaa gatatcccgc tcggacgagt gggtaaaccc gaagagttcg cggcggtcgt
     4141 cactttcctc gcaagccctc tcgccagcta tataacaggc gctaccatcc ccgtggacgg
     4201 cggcgcgatc atggcgacta cttaaaacct cgaggctgca gccggcattt cgtccggctg
     4261 cttactgacg atgttgatct gaacggccca gtcggccagc tgatacacca gctccagctc
     4321 cacttcaact ttgctgtaac gccagtcgtc gatccccagc cgcaaggtag aggaggtaaa
     4381 agcagcttct tccttcgcag gattccattc tccgctcaga tacacgagac tactgtcctg
     4441 caaacgcata taacaacctt tcagctgacg gtacaactgg tgataacatt tctccgcggc
     4501 attgaagtcg cccccgctgt acatcttcgc ctgccagctg gccgtcgtgt cgccggtcga
     4561 atgccagcgc gtaatgatac aatccgccga gcccggtaat tgaaccatcg aggcgtaatt
     4621 ttcgaactcg ccctgtgcca tcaccaggtc gccggtaatg tgccggagat tgttgggaaa
     4681 atcgcgaagg atggaatcga tggcggtcac gaatggcgat acagatgttt gcgcactcgc
     4741 tcctgcaacg gtcgcgaaaa agaaga
//
LOCUS       DASYSV010000420         5480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_209686, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000420 DASYSV010000000
VERSION     DASYSV010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209686"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..877
                     /locus_tag="VGQ51_12885"
     CDS             143..877
                     /locus_tag="VGQ51_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEV9037519.1"
                     /translation="MKRIAFLGAKGIGLECLKILDAKNFPIAALFTDLTRNKEANQKL
                     IDFAKERNIPVYEDLEAILSLPGFDYIISIQYHKILKGKHIGCAKKLALNLHMAPLPE
                     YRGCNQFSFAIVDGKKEFGTTLHKMAEGIDNGDIIAESRFDIPANCFVKELHEITYQK
                     SIRLFEDKISDILNDRYTLTPQDSLVPVRGTKTIYRKDIDKLKEINLDWDKDRIERHI
                     RATLMPGFEPPFFKMSDKKVYFSLFA"
     gene            888..1994
                     /locus_tag="VGQ51_12890"
     CDS             888..1994
                     /locus_tag="VGQ51_12890"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV9037520.1"
                     /translation="MKIPFVDLYAQYLSIKKEIDGAIETTIRNSSYIGGQAIKDFEQA
                     FAAFIGINQVIACANGTDSIEILLKAYGIGPGDEVIVPAISWISTSEAVSSVGATPVF
                     ADIERDYYTIDPELAEQAITPRTKAIIPVHLYGHPADMPRVMDIARRHNLIVIEDCAQ
                     AHGAAIDGRTAGTFGHAASFSFYPGKNLGAYGDAGAMATNDFAIAEKVRMIAQHGQKG
                     KHNHLIEGRNSRLDGLQAAILLAKLPHLEQWTEARIAVAKKYDARLEETGIAIPKVKK
                     NYRHVFHLYVTRVEDREDLQQKLSESGIETAIHYPTALPFLPCYSHRGFNPDNFPVAW
                     EYQHQILSLPLYPEISDDAINFVSAALKNCHVDQ"
     gene            1981..3150
                     /locus_tag="VGQ51_12895"
     CDS             1981..3150
                     /locus_tag="VGQ51_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037521.1"
                     /translation="MSINSRGKNIVILSTEPWGKMLLSKMHYANVLVEQGNRVFFINP
                     PRETKGSPLVDIVEQHAEGRLTIADIRAIAGSLFLRHKLFPVFKMISRRYARAIRRLV
                     GEPIDEVWCFNPHVFVDLRAFKAKRSILMIYDFYKGDHIFRTAATADAFITISQLILD
                     HYRECPPRKLLMQHGLSRSFATRAAGRLKDIEHAARPGNKIRVGYMGNLLRAGMNTDI
                     AREVITKHGDKEFHFWGPCSLEDNNVTPGGATTAADLVSFIEFLRGRDNVILHGMKDQ
                     ESLAEGLSTMDVFLFLYSPRTEMNSASNAHKILEYLSTGKVVVSTHVSNYAGTGLLTM
                     TDKDREEDFPALFETVTGRLSFYNDVDQQKKRIAFALDNTYSRQVERIQEFIYKP"
     gene            3150..4367
                     /locus_tag="VGQ51_12900"
     CDS             3150..4367
                     /locus_tag="VGQ51_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9037522.1"
                     /translation="MKKICIVTTRHISYNPRVLKEADTLHALGYRVTVVTINNHAGQS
                     QFDDELMQSRQWTLKTVNFRRTVPAEKRKWLRLSLKQRFFHLLSRITLRFGIAERTAE
                     KSFDDLAALAKDERADFYIAHHAEALGAASFAARANDTRFGFDAEDFHTGMEDMSPAY
                     YALITFLEQKYLPRCQYLTAAADGIAAAYADKYGVRRPATILNVFPRETTEQKEVHSP
                     VRFYWYSQVIGPKRGLEMLIAAAGRIHEPFELHLRGSWHNEAYKVELERLAVECGCAE
                     KLFFHDPILAKDIIGDAVNYDVGLGLELDVSINKYLAVSNKLFSYLMSGLALILTDTD
                     GQKGIYSAFPSAGRLCRMNDTESLAEAMRFYIENPGRLMEAKKAAREAAVTRFNWENE
                     SARLLENIKPCLA"
     gene            4364..>5480
                     /locus_tag="VGQ51_12905"
     CDS             4364..>5480
                     /locus_tag="VGQ51_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037523.1"
                     /translation="MKKVLIIAPHFPPSNLAAVHRSRLFAQHLPEYGWQPVILTVDEK
                     YYEEPPDLNLARLLPEDLEIEKVRAMRLTRPRLIGDIGLRAFFTMYRRAKKLIRKQQI
                     DFVYIPIPSFYVALLGRWLHATTGVPYGIDYIDPWVHRFPGSDRLFSRHWFTTKLAAV
                     LEPVAVKKASLITGVAEGYYQGVRSRNPLLEKTCLFGAMPYGGERADHEKVKELGLKP
                     YLFKPKPDKLQLVYAGAMLPKAYAPLEEIFRTMQEHPESVADLEIHFIGTGKTPNDPE
                     GYNIRPLAEQYGLWQTAVYEYPARIPYLDVLVHLEAAAGVFILGSTEPHYTPSKVYQG
                     VLSGKPLLAVLHRESTAASVIREAGAGIVLSFGSENAV"
BASE COUNT         1376 a         1541 c         1392 g         1171 t
ORIGIN      
        1 gctttaccaa tgaggaattc gatcaatata ttgcggcccc gcaggttgcc cacaaggttt
       61 atggcctttc tcaatccatg tatgaaaaat acccggtcct gaagattgcc aggccgatcg
      121 ttaatttatt aaaataggca ggatgaaacg gatcgctttc ctgggcgcta aagggatcgg
      181 gctggaatgt ctgaagatac tggatgcaaa aaacttcccg atagccgcac tgttcaccga
      241 cctgacgcgc aacaaagaag ccaatcaaaa gctgatcgat ttcgcaaaag agaggaatat
      301 cccggtatat gaagacctgg aagccatcct ctcgcttccc gggttcgatt atatcatatc
      361 catccaatat cataagatac tcaagggcaa acatatcgga tgtgcaaaaa agctggcgct
      421 gaaccttcat atggcgcccc ttcccgagta caggggctgt aatcaattct cctttgccat
      481 cgtcgacgga aaaaaggaat tcgggacgac gctgcacaaa atggcggaag gcatcgacaa
      541 cggcgatatc attgccgaaa gccggttcga catccccgcg aattgtttcg taaaagaatt
      601 gcatgagatc acctatcaaa aatccatccg gctcttcgaa gataaaattt cggacatttt
      661 gaatgatcgg tatacgctta ccccccagga ttcgttagtg ccggtccgcg gcacgaaaac
      721 gatctataga aaagacatcg acaaattgaa ggagataaac ctggactggg acaaagacag
      781 gatcgaaaga cacatacggg cgactttaat gcccggtttc gaacctccgt tctttaaaat
      841 gagcgataaa aaggtttatt tttcactctt cgcataacca atcgactatg aagatccctt
      901 ttgtagacct gtacgcccaa tatctcagta ttaaaaaaga aatcgatggg gcgatcgaaa
      961 ctaccattcg caactcctcc tatatcgggg gccaggcgat aaaggacttt gagcaggcct
     1021 tcgctgcctt catcggaatt aaccaggtga tagcctgcgc caacgggacc gattcgatcg
     1081 agatcctgct gaaggcttat ggcatcggtc ctggcgatga ggtcatcgtt cccgccatat
     1141 cgtggatatc cacctccgaa gcggtcagct cggtcggtgc gacacccgtc tttgcggata
     1201 tcgaaaggga ttattatacc atcgatccgg aattggccga acaggcgatc actccccgga
     1261 cgaaagcgat cattcccgtt cacctctacg gacatcccgc cgatatgccc cgggtcatgg
     1321 acatcgcccg cagacacaac ctcatcgtca tcgaagattg cgcacaggcg catggcgcgg
     1381 ccatcgacgg tcgcaccgcc ggcaccttcg ggcatgccgc cagcttcagc ttttacccgg
     1441 gtaagaacct tggcgcctat ggggacgccg gggcgatggc taccaacgat ttcgccatcg
     1501 cggaaaaagt gaggatgatc gctcagcatg gacaaaaagg gaaacacaac catctcatcg
     1561 agggtaggaa tagccggctg gacggtttgc aggcggcgat attgctggcc aagctgccgc
     1621 atctcgaaca atggacggag gcgaggatcg cggtcgcaaa gaaatacgat gcccgtctcg
     1681 aagagaccgg tatcgccatc ccgaaagtga aaaaaaatta ccgccacgtt ttccatctct
     1741 atgtcacccg cgtggaagac cgcgaagatc ttcaacaaaa attatccgaa agcggcatcg
     1801 agacggccat ccactatccc acggccctcc cattcctgcc ttgttatagc catcgcgggt
     1861 tcaaccccga caacttcccc gtcgcctggg aataccagca ccagatactc tctcttcccc
     1921 tttaccccga gatttcagat gatgcgataa attttgtcag cgccgcctta aagaattgtc
     1981 atgtcgatca atagccgggg aaaaaatatc gtcatccttt ccaccgagcc ctggggcaaa
     2041 atgctgctct cgaaaatgca ttacgccaat gtgctggtcg agcaaggcaa ccgtgtattc
     2101 ttcatcaacc ctccgcgcga gacaaaagga tcgccccttg tcgacatcgt cgaacaacac
     2161 gccgaaggcc gcctgaccat cgccgatatc agggcaatcg ccggcagcct gtttcttcgc
     2221 cacaagctgt tccccgtttt caaaatgatc agccgccggt acgcccgcgc tattagacgg
     2281 ctcgtcggcg aacccatcga tgaggtctgg tgcttcaacc cgcacgtgtt tgtcgacctc
     2341 cgcgctttca aggcaaaacg atcgatcctg atgatctatg atttttacaa gggcgaccac
     2401 atcttccgca ctgcggctac cgccgacgcc ttcatcacga tctcacaact gatcctcgat
     2461 cattaccgcg aatgtccccc gcgcaaactg ctgatgcaac acggcctctc ccggtccttc
     2521 gccacacgcg cggccggcag actaaaagac atcgaacacg ccgcccgtcc cggcaataag
     2581 atacgcgtcg gttatatggg taaccttctt cgcgccggga tgaatacgga catcgcccgc
     2641 gaagtcatca caaaacatgg cgacaaggaa tttcatttct ggggaccctg ctcgttggaa
     2701 gacaataacg ttactcccgg cggcgcgacg accgcggccg acctggtatc gtttatcgaa
     2761 tttctgaggg gtcgcgacaa tgtcatcctg catgggatga aggaccagga gtcgctggcg
     2821 gaaggattgt cgacgatgga tgtcttcctt ttcctgtatt ctcctcgaac ggaaatgaat
     2881 tccgcttcga atgcccataa gatactggaa tacctcagta ctggcaaagt agtcgtatcc
     2941 acccatgtct ccaattatgc cggcacgggt ctgctgacga tgacagacaa agaccgggaa
     3001 gaagactttc ctgcactgtt cgagaccgtc acgggacgtc tttccttcta caacgatgtg
     3061 gatcaacaaa agaaaagaat tgcattcgct ctggataaca cctattcccg gcaggtagaa
     3121 aggatacagg aattcattta taagccgtga tgaaaaagat ctgtatagtg acgacccggc
     3181 atatcagcta taatccccgg gtattgaaag aggccgacac gctgcacgca ctgggctata
     3241 gggtcacggt cgtcacgatc aacaatcacg ccgggcaaag ccagtttgac gacgagctga
     3301 tgcaatccag acagtggacg ctcaaaacgg tgaatttcag gagaacggtt cccgctgaaa
     3361 aaaggaaatg gctgcgtctt tcactcaaac agcgcttttt tcatctgctg tcgcggatca
     3421 cgttgcggtt cggcatagcc gaacgtacgg ccgaaaagag cttcgacgac ctcgcggccc
     3481 tcgctaaaga cgaacgggct gatttctata tcgcccatca tgccgaagcg ttgggcgccg
     3541 cttcgttcgc cgcccgggcc aacgacaccc gtttcggttt cgatgccgag gacttccata
     3601 cggggatgga agatatgtcg ccggcatatt atgcgctgat caccttcctg gaacaaaaat
     3661 accttccacg ctgccaatac ctgacggccg ccgccgacgg gattgccgcc gcctatgccg
     3721 ataaatatgg cgtcagacga ccggcgacca ttctgaatgt cttcccccgg gaaaccaccg
     3781 aacaaaaaga agtgcattca cccgtcagat tttattggta ttcgcaggtg atcgggccca
     3841 agagagggct tgagatgctg atcgcggcgg cgggacgcat acacgaaccc ttcgaattgc
     3901 atctgcgggg cagctggcac aatgaagcgt ataaggtgga gctggaaaga ctcgccgtcg
     3961 aatgcggatg cgccgaaaaa ctcttctttc atgaccccat actggcaaag gacatcatcg
     4021 gcgacgccgt taactatgat gttggactgg ggctcgagct ggacgtttcc atcaataagt
     4081 atctcgccgt ctccaataag ctattctcct acctgatgtc cgggctcgcc ctcatcctga
     4141 ccgatacgga cggacaaaaa ggaatctatt ccgcgtttcc ctcggcaggg cggctatgcc
     4201 ggatgaacga tacggaaagc ctcgccgaag cgatgcggtt ctatattgaa aatcccggcc
     4261 ggctaatgga agcaaagaag gccgccaggg aagcggccgt cacccggttc aactgggaaa
     4321 acgaatcggc cagattattg gagaatataa aaccctgcct ggcttgaaga aggttttgat
     4381 catagcacct cattttccac cgtcgaacct ggcggctgtc caccggtcac gcctgttcgc
     4441 tcaacacctg cccgaatacg gatggcagcc ggtgatcctg acggttgatg aaaaatatta
     4501 tgaagagccg cccgatctga atctcgcgcg tctgctgccg gaggacctgg agatcgaaaa
     4561 ggtccgggct atgcgcctga cacgcccgcg gctgatcggc gatatcggcc tgagagcctt
     4621 tttcaccatg taccgcaggg ccaaaaagct gatccgcaag caacagatcg atttcgttta
     4681 catcccgatc ccctcctttt atgtcgccct tcttggaaga tggctgcatg ccaccaccgg
     4741 agttccttat ggtatcgatt atatcgatcc ctgggtccac cgttttcccg gaagcgaccg
     4801 gctattttcc aggcattggt tcaccaccaa actggccgcc gtcctcgaac cggtagcggt
     4861 aaagaaagcg tcgctgatca ccggtgtcgc tgaaggctat tatcagggcg tacgatcacg
     4921 caacccgttg ctggaaaaaa cctgcctgtt cggcgccatg ccctatggcg gtgaaagggc
     4981 cgatcatgag aaagtaaagg aattgggctt gaagccctac ctgtttaagc cgaagcccga
     5041 taagttgcag ctggtctatg ccggagccat gctccctaag gcctatgcgc cgctggaaga
     5101 aatattccgg accatgcagg agcatccgga atccgtcgct gacctggaga tccatttcat
     5161 cggcaccgga aagaccccta atgatcccga aggctataac atccggccgc tggcagaaca
     5221 atatggattg tggcaaactg ccgtttacga ataccccgcc aggatccctt acctggacgt
     5281 gctggtgcat ctggaggccg ccgccggcgt gttcatcctg ggcagcacag agccgcatta
     5341 tactccttcg aaagtgtatc agggcgtgct cagcgggaag cctttgctcg cagtcctgca
     5401 ccgcgaaagt actgccgctt cagtgatccg cgaggcaggg gcgggtatcg tcctgtcttt
     5461 cggtagcgaa aatgcagtgc
//
LOCUS       DASYSV010000421         5773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_209972, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000421 DASYSV010000000
VERSION     DASYSV010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209972"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..1189
                     /locus_tag="VGQ51_12910"
     CDS             416..1189
                     /locus_tag="VGQ51_12910"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEV9037524.1"
                     /translation="MIKNGDKKRILITQPRPETDKSPYFELARKYDVELEFHPFIRLE
                     PIPAKEFRKQKIDISQYTAVIFTSRNAIDHFFRICEEMKIIISQDTKYFCITEAVALY
                     LQKFILYRKRKVFYGADGLNKSMFDVINKHKENEKFLYPCSENQQDNEIVNWLKCNNC
                     GFATPFMYRTISNDVTSVLNQSEYDIICFFTPSGVRSLFDNYPKFKQNGTKIGAFGNN
                     TSRAVEDAGLTLDIKAPQPQAPSMVSALEKYLAVTIKKK"
     gene            1215..3287
                     /locus_tag="VGQ51_12915"
     CDS             1215..3287
                     /locus_tag="VGQ51_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEV9037525.1"
                     /translation="MENGPRHTNRLSGETSPYLLQHSHNPVDWYPWGEEALQIARSQD
                     KPILVSIGYSACHWCHVMEKESFEDEATARFMNEHFVNIKVDREERPDIDHIYMDAVQ
                     AMTGAGGWPLNVFLTPDARPFYGGTYFPPRPIYNRGSWKDVLAGVAAAFRDRREEVEQ
                     HAETLTRHVETAGNFGIGDGAPATNAPFRRSTLEQIKGQLLASADKTAGGFGQAPKFP
                     QTFSIRYLLYDYAITGDTGALDQACLSLDSMIRGGIYDQLGGGFARYSTDDRWLVPHF
                     EKMLYDNALLIIATSEACQLISRLPDATGRAALYREAIGQTIRFAERELSNGKGAFFA
                     ALDADSEGVEGKYYVWDKKEIDTVLGEDADTFNRYYGVTAAGNWEGKNILTRPSDAPV
                     PAEQHEKLESARLRLLEHRAGRVPPGLDNKILLDWNALMNIALSKAFAALGDDRYRDM
                     AVDNMRFLRDHLRGDNGQWFHARKGKTEIPAFLDDYAALIAALLQLQEITGETAYLLQ
                     AEDILRFVIGHFGDPETGFFFYTHSGQQDILVRKKEIYDGATPSGNSMMASNLLYLSI
                     VFDQPEWARRANQMASALGRVVTAYPGSFGGWATIFQAFTYSIAEVVITGRKPENARK
                     EFLAHLVPFRVFQSAQEENTQFPLLRDKPVEAGPLIFLCKDYACQLPVNEVSTAIRLI
                     ENVQKFQE"
     gene            3469..4749
                     /locus_tag="VGQ51_12920"
     CDS             3469..4749
                     /locus_tag="VGQ51_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEV9037526.1"
                     /translation="MKNLSALTAFFALFLMAGCKLGGKSGKGLANDGQLHGVAPAGAY
                     NLSKPPGMVYIPPGTFHMGPSDEDMNYAFTARNKQVSISGFWMDATEITNNEYRQFTN
                     WVRDSIAATLMQYGKDVDGRFAIDWKKTATIKWGDKATVEKLNQMIVEPDNRIFGKKE
                     IDPNKLVYHSETFDLKAAAQQAAEGKPRSQFIVKKDIKIYPDTLVWIRDFSYAYNEPM
                     TKRYYSHPAFGNYPVVGVNWNQATVFCEWRTQYLNSFLESKHLATESNFRLPSEAEWE
                     YAARGGRSQSMYPWGNYYLRNKRGCLLANFKPGRGNYPEDGGFYTVRADAYWPNDFGL
                     YCMAGNVAEWTASFYYEGSSNFQHDMNPDIRWNAKDSDPPRMKRKVIRGGSWKDVGLY
                     LQTSARTYEYQDTAKSYIGFRCVIDLPPTMTGKR"
     gene            4911..5684
                     /gene="gldL"
                     /locus_tag="VGQ51_12925"
     CDS             4911..5684
                     /gene="gldL"
                     /locus_tag="VGQ51_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HEV9037527.1"
                     /translation="MSAGVSKSTERLINIIVCVGAAVVIFGALQKILHTKLADFFLTA
                     GLLTEALIFLVYAFLPPPGGEMHALVEALPKLAGGTSGNPSLDKMDKMLQEADITPAN
                     LNRLSEGFKKFGTTVDNIRDASGALAATTEYTNKTKEAAAALDGMKGAYQNATNTISS
                     FNAAADSTKQFHSQVQVLTKNLGSLNAIYELELQDTNNHLKAMNSFYGNLVKASETMQ
                     GSVADAKKTQEQIALLAKNLGNLNQVYGNMLSAMQGRPA"
BASE COUNT         1496 a         1527 c         1429 g         1321 t
ORIGIN      
        1 gtcgtctttt tggtgaacaa aatgctggga attttcctgc ttcctttctt gatcctgatc
       61 actttttcgg ataaccaggc ccgggaaata ttcataacta tttcattggt aatggtattc
      121 gccatgtggg tctataggac catggcggcc taccgccccg tgcgcaacga aataaaacta
      181 acgccgtttt atttttttct gtacctttgc gccttcgaaa tagcacccgt tctattgatt
      241 tacaaggtgt tattgacata tttggaaaaa gtgtattaac tttgtgtacc tcggcggtca
      301 aaacggaccg gaggtccgtt ggccgaaggc caataattag cgttttgcag gagatgcgct
      361 aattggcaca aagtgcggct cttgcgatta acttactaat ccagaggggg accacatgat
      421 taaaaacggg gataaaaaaa gaattttaat cactcaaccc aggcctgaaa cagacaagtc
      481 gccgtatttc gagctggcca ggaagtatga tgtagaattg gagtttcatc ctttcatccg
      541 tctggaacct attccggcca aggaattccg caaacagaag atcgacatct ctcaatacac
      601 cgcggtcatc ttcaccagcc gcaacgccat cgatcatttc ttccggatat gcgaagaaat
      661 gaagataatc atctcccagg acacaaaata tttctgtatc accgaagcgg tcgcgcttta
      721 tctccaaaag tttattcttt accgtaagcg aaaggtattc tatggtgccg acggtctcaa
      781 caagagcatg ttcgacgtga tcaacaaaca caaggagaac gagaaattcc tctatccctg
      841 ttccgagaac cagcaggaca acgagatcgt caactggctg aaatgcaata attgcggttt
      901 cgctacaccc ttcatgtatc gcacgatcag caacgatgtc acttccgtcc tcaatcaaag
      961 cgagtacgac atcatctgtt tctttacccc aagcggcgtc agaagccttt tcgacaacta
     1021 cccgaaattt aaacagaacg gcactaagat aggcgccttc ggcaacaaca cctcgcgtgc
     1081 ggtcgaagat gctggtctta cgctggatat caaggccccg caaccccagg caccctccat
     1141 ggtctcggcg ctggagaaat acctggccgt cacgatcaag aaaaaataag ccgcgcagcc
     1201 cttaacttta tgcgatggag aatgggcccc gccataccaa tcgtctctca ggggagacga
     1261 gtccttattt attacagcat agccataacc ccgtggattg gtatccatgg ggcgaagagg
     1321 cattgcagat agcgcgttca caggacaaac cgatcctcgt cagcatcggc tattccgcct
     1381 gccactggtg tcatgtgatg gaaaaggaaa gtttcgagga tgaggcgacc gcccgcttca
     1441 tgaacgaaca cttcgtaaat attaaggtag accgggaaga gcggccggac atcgatcata
     1501 tctatatgga tgcggtacag gccatgaccg gggcaggcgg ctggcccctg aacgttttcc
     1561 tgactcccga tgccagaccg ttctatggcg gcacctattt ccctccgcga cccatctata
     1621 acaggggttc ctggaaagac gtgctggcgg gtgtcgccgc ggcttttcgg gacagacgtg
     1681 aagaagtcga acaacacgcc gaaaccctta cccgtcatgt agagacggct ggcaacttcg
     1741 gaatagggga cggggccccc gcaacaaacg caccgttccg gcgatctacc ctggaacaaa
     1801 taaagggtca gttactcgca tctgcagaca agacagcagg aggattcggc caggcgccaa
     1861 aatttcccca gaccttctcc atccgttatc tcctttatga ttacgcgatc accggcgata
     1921 ccggggccct tgaccaggcc tgcctgagcc tcgactcgat gatcaggggc ggtatctacg
     1981 atcagctggg cggcgggttt gcccgctatt ccaccgacga ccggtggctg gttccacatt
     2041 tcgagaagat gctatacgat aatgctttgc tgatcatcgc gaccagcgag gcctgtcagc
     2101 tcatctcccg gctcccggac gctaccggta gagcggccct ctatcgcgag gccatcgggc
     2161 aaaccatccg attcgccgaa cgtgagctgt ccaacggaaa aggggcgttc tttgcagcgc
     2221 tggacgccga tagtgaagga gtcgaaggga aatactatgt atgggacaag aaagagatag
     2281 ataccgtcct gggagaagac gccgatacgt tcaacagata ttatggtgtc accgcggccg
     2341 gcaactggga ggggaagaat atcctcactc gtccttccga tgccccggtc cccgcggaac
     2401 aacatgaaaa gctcgaatcc gcccgtctcc gcctcttgga acatcgcgcg ggaagggtcc
     2461 cgccgggatt ggacaacaag atcctgctcg actggaatgc gctgatgaat atcgcgcttt
     2521 cgaaggcttt cgccgccctg ggtgacgacc gctatcgtga catggccgtc gacaatatgc
     2581 ggtttctcag agaccacctg cggggggata atggtcaatg gttccatgcc cggaaaggga
     2641 agacagaaat acctgcattc ctggatgatt atgccgctct gatcgcagcg cttctccagc
     2701 tccaggagat caccggcgag acggcctacc tgctacaggc ggaggatatc ctccggtttg
     2761 tgatcgggca tttcggtgat ccggagaccg ggttcttctt ttatactcac agcggacagc
     2821 aggatatcct tgttcggaaa aaagaaattt acgatggagc tactccttcc ggaaactcta
     2881 tgatggcatc taatttgtta tacttatcta tcgttttcga tcagccagaa tgggcgcggc
     2941 gagccaacca aatggcttcc gccctgggaa gggtggttac ggcctatccg gggtctttcg
     3001 ggggatgggc aaccatattt caagccttta cgtattctat tgcggaggta gttatcaccg
     3061 gcagaaagcc ggaaaatgcc cggaaggagt ttctagcgca cttggttcct ttccgggttt
     3121 tccagtctgc gcaggaagaa aatacccaat tccccttatt aagagacaaa ccggtcgagg
     3181 ccggtccgct gatcttccta tgtaaagact atgcttgtca actccctgta aatgaagtaa
     3241 gtacagcgat ccgtctcatc gaaaatgtgc aaaagtttca agaataaacg tacaataaac
     3301 cgaagtcggt gacgtttatg tccttagcgt taagaaaggc ttggccgtcc gcccggtatc
     3361 gtatattttc agcggatgta cgctttgact ttaaaaaaat tattttaaac ttgtagcgag
     3421 atgttgtcaa acgatttttt ttgcccaata ccctttaata ttatgaaaat gaaaaaccta
     3481 tccgctttaa cggccttttt cgccctcttc ttgatggcag gctgtaaatt aggtggaaaa
     3541 tccggcaaag gattagcgaa tgacggccag ctgcatggcg tcgctcccgc gggtgcttac
     3601 aatctttcca aaccccctgg catggtctat attccgccgg gcaccttcca catggggccc
     3661 agcgacgagg atatgaacta tgcttttact gctcgcaaca aacaggtgtc gatcagtggt
     3721 ttttggatgg acgcaaccga gatcaccaac aacgaatacc gccagttcac caattgggtc
     3781 agagattcca tcgctgctac cctgatgcaa tatggtaagg acgtggatgg ccgtttcgct
     3841 atcgactgga aaaagactgc gaccatcaaa tggggtgaca aggctaccgt cgagaagctc
     3901 aatcaaatga tcgtggagcc cgataaccgt atcttcggta agaaagagat cgatcccaat
     3961 aagctggtct accattccga gaccttcgat ctcaaggctg ccgcacagca ggctgccgaa
     4021 ggtaaacccc gctcacagtt catcgtgaag aaagacataa agatctatcc ggataccctg
     4081 gtttggatcc gggacttcag ctatgcttac aatgagccca tgaccaagcg gtattactcc
     4141 caccccgcct tcgggaacta tcccgtagtt ggggtcaact ggaaccaggc taccgtcttt
     4201 tgcgaatggc gtactcaata cctcaactcc tttttggaaa gcaaacacct ggctactgag
     4261 tctaatttcc gtctgcccag cgaggccgaa tgggaatatg cggctcgcgg tggtcgttct
     4321 cagtcgatgt acccctgggg taactactat ctgagaaata aaaggggctg tctgctcgcc
     4381 aatttcaaac caggcagggg caactatccc gaagacggtg gtttctatac cgtccgggca
     4441 gatgcctact ggcccaacga cttcggtcta tattgtatgg ccggcaacgt cgcagaatgg
     4501 actgcctcct tctattatga aggctcttcc aacttccagc acgatatgaa cccggacatc
     4561 cgctggaatg ccaaagactc cgatcccccc cgtatgaaac gtaaggtaat tcgtggcggt
     4621 agctggaaag acgtagggtt atacctgcag accagcgccc gcacctatga gtaccaggac
     4681 acggccaaat cgtacatcgg gttccgttgt gtgatcgacc tgccgcccac gatgacaggc
     4741 aagcggtaag cagacgcgga gacggaaaac cctggtggca ttctttccgg gtacgcaaaa
     4801 ttttttcgcc aaaacaacgt tttgaatttg actaactaac aattaatcaa taggaactct
     4861 tttttccata accctttaaa atccttgtaa acaattaaaa attaatcact atgagcgctg
     4921 gagtatcaaa atctacagaa agactgatca atattattgt gtgcgttgga gcagccgtcg
     4981 tgatcttcgg cgccctccag aagatcctgc acaccaaact ggccgacttc ttcctgaccg
     5041 cgggtctgtt gacggaagca ctgatcttcc tcgtgtatgc cttcttaccg cctcccggag
     5101 gtgaaatgca tgccctggta gaagcccttc ccaaactggc gggcggcacc agcgggaacc
     5161 cgtcgcttga caagatggac aaaatgctac aggaagccga tatcactccg gcgaacctga
     5221 accgtctgag cgaaggtttc aagaagttcg gtaccaccgt tgataatatc cgcgacgcat
     5281 ccggcgcact ggccgctacg accgaatata ccaacaagac caaagaggct gccgcagccc
     5341 tggatggcat gaagggcgcg tatcagaacg cgaccaacac gatctccagc ttcaacgccg
     5401 ctgcagacag cacgaaacag ttccattcgc aggtacaggt cctgaccaag aacctgggct
     5461 cgctgaatgc catttatgaa ttggaattgc aggataccaa caaccacctg aaggccatga
     5521 acagcttcta tggcaacctg gtgaaggcat ccgagaccat gcagggtagt gttgccgatg
     5581 cgaagaagac gcaggagcag atcgcgctgc tggccaagaa cctgggtaac ctgaaccaag
     5641 tctatggtaa catgttaagc gccatgcaag gccgtccggc ataacaatcc aatttgtgcc
     5701 tgagcccgtt ttatccggcc cgcatgaacc taatatgaaa aacttattct aaccagaata
     5761 taatattact gac
//
LOCUS       DASYSV010000422         8048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1367836, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000422 DASYSV010000000
VERSION     DASYSV010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367836"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VGQ51_12930"
     CDS             <1..772
                     /locus_tag="VGQ51_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEV9037528.1"
                     /translation="DIKYILGHNPLFPAYSAQDPTATTSFAQKTAFLSVAAGLYDIGY
                     EGNSQPADRSGVRHESDGFCFDNELSRHKVYLDGYRISDSPVTNEEWLAFMEDGGYRN
                     FAWWHAEGWDWVKNNAVVAPLYWHPINGQWHQYTLSGLQPVDRRQPVCHVSYYEAVAF
                     AAWKGARLPTEQEWEVASGRFEWGQRWEWTESAYLPYPGFKKAAGAIGEYNGKFMVNQ
                     MVLRGASVATPPGHSRKSYRNFFQPQLRWQFTGIRLAK"
     gene            805..1785
                     /gene="egtD"
                     /locus_tag="VGQ51_12935"
     CDS             805..1785
                     /gene="egtD"
                     /locus_tag="VGQ51_12935"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEV9037529.1"
                     /translation="MFSFNTAIPTARRSANFFKDVTDGLRAEHKYLPSKYFYDEAGDK
                     LFQQIMACPEYYPTRCELDILQHQSAKMAHFFLDETPSFDLVELGPGDASKSWFLLRE
                     LQKEHADFTYYPIDISSNVIGWLEDRLPVTLQGLRIQGLNGEYLEKMHQLSTFSNNRK
                     IVLFMGANIGNMTIQQATQFCRQLHDHMQPGDLLLIGFDLKKHPRTVLDAYNDRQGIT
                     RAFNLNLLRRINRELGGDFDTEQFEHYPVYDPGTGACKSYLISRIAQEVHIADKRIVF
                     AENEPIFMEISQKYTLEEIGLMAAHSGFSPVHTFFDQKNWFADVLWQCQA"
     gene            complement(1814..2437)
                     /locus_tag="VGQ51_12940"
     CDS             complement(1814..2437)
                     /locus_tag="VGQ51_12940"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV9037530.1"
                     /translation="MNFSAYIEKSLTYSAYERLIADLLGQGRTTGPDQSEAFIHYTLL
                     NQQRMHRLDKTTPLLPSVETAIKSITVGQTWLVLTEAWCGDAAQSIPVMNAMAILNPV
                     ISLRLVLRDDNPDLMDRHLTNGVARSIPKLIGLDTATMEELFTWGPRPAALQERFYKM
                     KADGVEYHQAKEELQRWYNKDRTISIQEELAALASGSSSRAKVGMIA"
     gene            complement(2473..3120)
                     /locus_tag="VGQ51_12945"
     CDS             complement(2473..3120)
                     /locus_tag="VGQ51_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV9037531.1"
                     /translation="MTIGIMGSAEVGRALGHGFLAEGHPVMMGSRNPKKEELVKWQRD
                     HAAAKTGDFRDTARFGEILVLCVPGTAVEEVIRLAEPENFRGKIVIDVTNPISPGAPI
                     NGVLPFLTGPNESLMERTQRLLPDAKLVKAFNSVGSDLMYKPKFPGGTPTMFICGNDE
                     AARATVTDILTAFGWETEDMGKAEAARPIEALCMLWCIPGFLHNQWTHAFKLLKP"
     gene            3172..4203
                     /gene="ruvB"
                     /locus_tag="VGQ51_12950"
     CDS             3172..4203
                     /gene="ruvB"
                     /locus_tag="VGQ51_12950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV9037532.1"
                     /translation="MPNLNLNTDKNALSPSDKEFENNIRPSVIEEFAGQPQLIENLRV
                     FIKAAKMRGEALDHILFHGPPGLGKTTLSRIVANEMGVNIRETSGPVIEKPGDLAGLL
                     TNLGANDVLFIDEIHRLSTVVEEYLYSAMEDYRIDILIDSGPNARSIQLSLQPFTLVG
                     ATTRSGLLTAPLLSRFGIKSRLEYYTATTLQHIILRSASILHTKVTSDAAAEIARRSR
                     GTPRIANGLLRRVRDFAQVLAGGIVDRAITQHALRALHVDEHGLDEMDNRILSTIIEK
                     FKGGPVGITTIATAVGEETGTLEEVYEPFLIQEGFIMRTPRGREVTHKAYEHLGKKKP
                     GREGEQLLF"
     gene            complement(4226..4930)
                     /locus_tag="VGQ51_12955"
     CDS             complement(4226..4930)
                     /locus_tag="VGQ51_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9037533.1"
                     /translation="MEGKKPKILLCEDDPNLGMVLKNYLELSDFDVTLERDGRLGLAA
                     FQREKFDLCLLDIMMPHMDGFTLAEEIRDVDPDIPLFFLSAKTMKEDIIQGYKLGADD
                     YITKPFDSEVLLMKIKAILKRNDDLNKESDNKVFDLGTYHFNPKLRELAHKGQAQTLS
                     PKENELLKMLAEHMNDLLPREQALKKIWGSDTYFNGRSMDVYIAKLRKYLKDDSQIEI
                     VNIHGNGFRLVVPVNA"
     gene            5215..6621
                     /locus_tag="VGQ51_12960"
     CDS             5215..6621
                     /locus_tag="VGQ51_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HEV9037534.1"
                     /translation="MDANFNPDKQHTLASVTSISGTGLHTGIKVDMTLKPANPGFGFQ
                     FQRIDLPGQPVIKADCDLVTDTSRGTTLEDNGAKVSTVEHILAGLVGMGVDNCLIEIN
                     GPEVPIIDGSSQPFVEIIEEAGVTEQEATKAWYSIDSNIAYYDEKKRVEMVVMPSIEY
                     KVTTLIDFNSPVLGTQHAGLKTMREFKQEISPCRTFCFLHELEMLLEHNLIKGGDINN
                     AIVVVDKPVTKDEMGRLAKAFKRDKIEIKSEGYLNNLELRFPNEPARHKLLDVIGDLA
                     LIGYPIKGHIIANRPGHSTNVEFAKKIKQYIKKNKHLKNTPIYDPNQAPVFTLQQIEK
                     TLPHRFPFLLVDKIIELTDRHIVGIKNVTYNEPFFVGHFPGNPVMPGVLQIEALAQTG
                     GILCINAMPQGEYDTYFIKIDNCKFKHKVVPGDTMILKMELLNPIRRGICEMRGTVYV
                     GNKIATEADLMAQLVKRS"
     gene            6699..7577
                     /gene="lpxA"
                     /locus_tag="VGQ51_12965"
     CDS             6699..7577
                     /gene="lpxA"
                     /locus_tag="VGQ51_12965"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEV9037535.1"
                     /translation="MKRANRNEEIVMIQQTENQSTNHRTIHSLTSVHPDARLAPDVRV
                     DPFTMIHRNVEIGAGTWIGSNVTIMEGARIGKNCRIFPGAVVSADPQDLKFEGEETTV
                     EIGDNTTIREFVTVHRGTRDRWRTKVGRNCMILAYSHIAHDCIIGDNCILSNNSQVAG
                     HVTLGDWVILGGMCAIHQFVRVGSHVFLGGGSLVGKDIPPYIKAARMPLSYAGVNSVG
                     LKRRGFTIDKINHILDIYRVIYNKGMNTSQALEFLEEEFPASDERDEIVGFIRESGRG
                     IIKRFSKSSFDEDYAD"
     gene            7558..>8048
                     /locus_tag="VGQ51_12970"
     CDS             7558..>8048
                     /locus_tag="VGQ51_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9037536.1"
                     /translation="MKITLIDAGKRFNRDWIFRHLHYEFNTGCKYAITGPNGSGKSTL
                     LQSIAGAIAISEGTIDYAAPAATAKPASAKPASQASATTAKPAIPASTAVPPDQAYRY
                     LSLAAPYLSPIEEMTVTEFLDFHKIFKPFLPNLTTPDIIRTVGLETAAHKQIRFYSSG
                     MKQR"
BASE COUNT         1894 a         2321 c         2010 g         1823 t
ORIGIN      
        1 ggatatcaaa tatatattgg gccacaaccc gctattccct gcatattcgg cgcaggaccc
       61 gacggcaacg acatccttcg ctcagaagac ggcttttttg tccgtcgcgg cgggactata
      121 cgacatcggg tatgaaggca attcccagcc cgccgatcgc tccggcgtca ggcatgaaag
      181 cgacggtttc tgtttcgaca acgagctgtc gcggcacaag gtctacctcg acggatatcg
      241 tatttcggac tcgcccgtta ccaatgaaga atggctcgcg ttcatggagg acggcggcta
      301 caggaatttc gcctggtggc atgcggaggg ctgggattgg gtgaagaata atgccgttgt
      361 ggccccgttg tactggcatc ccatcaacgg tcagtggcat cagtataccc tgtccggtct
      421 tcagccggtg gacaggcgcc agccggtgtg ccatgtcagc tattatgagg ccgtggcctt
      481 tgccgcctgg aaaggcgcgc gtctgcccac ggaacaggaa tgggaggttg catccggtcg
      541 gttcgaatgg ggccagcgat gggagtggac cgaaagcgct tatctgccct atcccggttt
      601 taaaaaggcg gctggagcca tcggggaata taacggaaag ttcatggtga accagatggt
      661 gctgagaggc gcgtcggtgg cgactccccc cggacatagc cgcaaaagct atcggaactt
      721 tttccaaccc cagctgaggt ggcagttcac tggcatacgt cttgcaaagt aatcaaacac
      781 cgcaatccat aaaacgaagg tcttatgttc tcattcaaca ctgccatacc gaccgccaga
      841 cggtccgcca attttttcaa agacgtcacc gacggtctgc gcgccgagca taaatatctt
      901 ccgtcgaaat atttctacga cgaggccggg gacaaactct tccagcagat catggcctgc
      961 ccggagtact accccacccg ttgtgaactg gatatcctgc aacatcagtc tgctaaaatg
     1021 gcgcattttt tcctggatga aaccccttcc ttcgacctgg tcgaattggg tcccggcgat
     1081 gcctccaaat cgtggttcct gttgcgcgag cttcaaaagg aacacgccga ttttacatat
     1141 tatcctatcg atatctcttc caatgtcatc ggctggctgg aagatcggct gcccgttacg
     1201 ctgcagggtc ttcgcataca gggtctcaac ggagaatacc tggaaaaaat gcaccagctc
     1261 agcacgttct ccaataaccg taagatcgtg ttgttcatgg gcgccaacat cggtaatatg
     1321 acgatccagc aggcgaccca attctgccgc cagctgcatg accatatgca gcccggcgat
     1381 ctgctgctca tcgggttcga tctgaaaaaa cacccgcgca ccgtcctcga cgcctataac
     1441 gaccgccagg gaattacgcg cgccttcaac ctcaatctgc tgcggcgcat caatcgcgag
     1501 ctgggaggcg atttcgacac cgagcaattc gaacactatc ccgtttatga tcccggcacg
     1561 ggagcctgca aaagctacct gatcagccgc atcgcacagg aggtgcacat tgccgacaaa
     1621 cgcatcgtct tcgcggagaa tgaacccatc ttcatggaga tatcacaaaa atatacgctc
     1681 gaagagatcg ggctgatggc tgcccattcc ggattttcgc cggtgcatac ttttttcgac
     1741 cagaaaaatt ggttcgccga cgtattgtgg caatgccagg cctgaacgct tcacggcccc
     1801 ccggccttag agcctatgcg atcatcccga ccttcgccct cgaagacgag ccggatgcca
     1861 gcgcggccag ttcctcctgg atggatatgg tccggtcttt attataccac cgttgcagct
     1921 cctccttggc ctgatgatat tcgacgccat ccgccttcat tttataaaac cgctcctgaa
     1981 gcgccgcggg ccgcggcccc cacgtgaaca gctcctccat cgtcgccgta tcgagaccga
     2041 tgagcttcgg tatgctcctg gctaccccat tggtcagatg ccgatccatc aggtcggggt
     2101 tatcatcgcg taacaccagc cgtagcgaga tcacgggatt caggatcgcc atcgcattca
     2161 tcacgggaat actctgggcg gcatccccgc accaggcctc cgtcagcacc agccaggtct
     2221 gtccgacggt gatcgatttg atcgccgttt ctacggaggg cagcaacggc gtcgttttgt
     2281 ccagccggtg catgcgttgc tgattcagca gtgtataatg gatgaaggct tcggattgat
     2341 ccggtcccgt agtacggccc tgacccagca gatcggcgat cagccgttca taagctgaat
     2401 atgtcaggga tttttcgata taggcgctaa agttcatgtt atagtttcag acattggcgg
     2461 cactgatcgc actcatggtt tcaggagttt gaacgcatgc gtccattgat tgtgaaggaa
     2521 gccgggtata caccagagca tacacagcgc ttcgatcggc ctcgccgctt cggcctttcc
     2581 catgtcttcg gtctcccagc caaacgcagt gaggatatcc gttaccgtgg ctctggccgc
     2641 ttcatcattg ccgcagatga acatggtcgg tgtgccgccg gggaatttcg gtttatacat
     2701 gagatcgctg ccgacgctgt tgaaggcctt gaccagcttg gcatcgggaa gcaggcgttg
     2761 tgtccgctcc atcagcgact cgtttggccc ggtcaggaag ggcaacacgc cgttgatggg
     2821 tgcgcccggg gaaatggggt tcgtgacatc gatcacgatc ttgccccgga aattttccgg
     2881 ttccgcaagc cggatgactt cttccacagc ggtgccggga acgcaaagga ccaggatctc
     2941 tccgaaccgg gccgtatccc ggaaatctcc ggtcttcgct gccgcatgat ctctctgcca
     3001 ttttacgagc tcttcttttt tgggatttcg cgagcccatc atgacgggat ggccttccgc
     3061 cagaaaacca tgtcccagag cccgaccgac ttccgcggac cccatgatac cgattgtcat
     3121 aagtaaagtt ttgaagatgt aatttactct aattccgggt atctttgtcc catgcccaat
     3181 ctcaacctca acacggataa gaacgcttta agcccttccg acaaagagtt cgagaacaat
     3241 atccggcctt ccgtcatcga agagttcgcc ggtcagccgc agctgatcga aaatcttcgc
     3301 gtctttatca aggcggcgaa aatgcgcggt gaagccctgg atcatatcct tttccatggc
     3361 cctcccggtc tgggcaaaac taccctgtcg cgtatcgtcg ccaatgaaat gggcgttaac
     3421 attcgtgaga cttccggacc cgtcatcgag aaacccggtg atctggccgg tctcctaacc
     3481 aatctcggag ccaacgacgt gttgttcatc gatgagatcc accgtctgag cactgtcgtc
     3541 gaagaatatc tgtattccgc catggaagat tatcggatag atatcctcat cgacagcggt
     3601 cccaatgcgc gcagtattca gctctcgctc cagcccttca cgctggtggg cgccactacc
     3661 cgcagcggtc tgcttacggc cccgctgtta tcccgtttcg ggatcaaatc cagactggaa
     3721 tattatacgg ctacgactct tcaacacatc atcctccggt cggcatctat cctgcacacg
     3781 aaggtcacct ccgatgcggc cgcggagatc gcccgtcgca gccggggtac tccccggatt
     3841 gccaacgggc tgctgcgtcg tgtccgggat ttcgcacagg tcctcgccgg cggaatcgtc
     3901 gatcgtgcca ttacccagca cgccctccgg gcgttgcatg tagatgagca tggcctggac
     3961 gaaatggata accgcatcct gtctaccatc attgaaaaat ttaagggcgg tcccgtgggg
     4021 atcaccacca ttgctaccgc cgtcggggaa gagaccggca cgctggaaga ggtatatgag
     4081 cctttcctta tccaggaagg attcatcatg cgtacgcccc gcggacgcga ggtcacgcac
     4141 aaggcctacg aacacctggg taagaaaaag cccggcaggg aaggggagca gctgctgttc
     4201 tgaacgtcac cgccgcgccg ccaatctaag cgttcaccgg cacaacgagc cggaacccat
     4261 tcccatgtat attcacaatc tcgatctgac tatcatcttt cagatacttc ctcagcttcg
     4321 cgatatacac atccatgctt cgaccgttga aatatgtatc cgacccccag atctttttca
     4381 gtgcctgctc ccgtggcagc agatcgttca tatgctcggc cagcatcttg agcagctcgt
     4441 tctccttcgg agataaggtc tgcgcctgcc ccttatgagc cagctcacgc agcttgggat
     4501 tgaaatgata agttccaaga tcgaatacct tgttgtccga ttccttgttc agatcgtcat
     4561 tgcgtttcaa tatcgccttg atcttcatca gcagcacctc gctatcgaac ggcttggtga
     4621 tataatcgtc ggcgcccaat ttatatcctt gtatgatatc ttccttcatc gtctttgcac
     4681 taaggaagaa aagcggaata tccggatcca cgtcacgtat ctcttcggca agcgtaaagc
     4741 cgtccatatg cggcatcatg atgtcgagca gacagagatc gaacttctcg cgttggaacg
     4801 ccgccagccc cagccggccg tctctttcca gtgtcacgtc gaaatcgctt agctcaaggt
     4861 aatttttcag taccatgccc agattcggat cgtcttcgca caacaatatt ttgggtttct
     4921 ttccttccat agttatgatt ttagttacaa ataaaattaa atcattgcct gataatcaac
     4981 gcttgaggct aatattttgt taaaccgccg tatttttgag cctgtgcgcc tcacttcaga
     5041 caataaattt aagaaactag ccgtaattgg cgatcgggtt ctcatccgtc cctctcgcgg
     5101 gccgaccgcc aacgccgttt gacggcccgt ccccgggtaa aaccaaccgc tattttcccc
     5161 tatctttgtc ggccaaagac gggcgccctc aattgatatc ttttaaaaaa acgcatggac
     5221 gcaaatttca atcctgacaa acaacacaca ttagcctctg ttacaagtat ttcgggtacg
     5281 ggcctgcaca ccgggatcaa ggtcgatatg accctgaagc cggccaaccc gggtttcggc
     5341 ttccagttcc agcgtataga cctgccgggt cagcccgtga taaaggcaga ctgcgacctg
     5401 gtgaccgata cttcccgtgg cacgactctc gaggacaacg gggcaaaggt cagtacggtc
     5461 gagcatatcc tggcaggtct cgtggggatg ggcgtggata attgtctgat cgagatcaac
     5521 ggtccggaag tgcccatcat cgacggcagc tcgcagcctt ttgtcgagat catagaagaa
     5581 gcgggtgtta cggaacagga ggcgaccaag gcctggtaca gcatcgattc caatatcgcc
     5641 tactatgacg aaaagaaaag ggtggagatg gtggtcatgc cttcgatcga gtataaggtg
     5701 acaaccctga tagatttcaa cagcccggtc cttggaacgc agcatgccgg tctgaagacg
     5761 atgcgcgaat tcaaacagga aatatccccc tgccgtacct tctgtttcct gcacgagctg
     5821 gagatgctgc tcgagcacaa cctgatcaag ggcggggaca tcaacaacgc catcgtcgtc
     5881 gtcgacaaac ccgtgacgaa agacgaaatg ggccgtctcg ccaaagcgtt caaacgggac
     5941 aagatcgaga tcaaaagcga aggttacctg aataatctcg agctgcgttt tcccaatgaa
     6001 cccgcgcgtc acaagctgct ggacgtcatc ggcgatctcg cgctcatcgg ctatcccatc
     6061 aagggacata tcatcgccaa ccgcccgggc cattcgacca acgtcgaatt cgccaaaaag
     6121 atcaagcaat acataaaaaa aaacaaacat ctaaagaata cacccatcta tgaccccaac
     6181 caggcgccgg tctttacctt gcaacagata gaaaagaccc tgccgcaccg gttccccttt
     6241 ctcctcgtcg acaagatcat cgagctaacg gacaggcata tcgtcgggat caagaacgtc
     6301 acctacaatg aacccttctt cgtcggtcat tttcccggta accccgtgat gcccggcgtg
     6361 cttcagatag aggcgctggc ccagacgggc ggtatcctat gtataaacgc tatgccccag
     6421 ggtgagtacg atacctattt tatcaagatc gataattgca aattcaaaca taaggtcgtg
     6481 cccggtgaca cgatgatcct gaaaatggag cttctcaatc ccatacgccg gggcatctgc
     6541 gaaatgcgcg gcacagttta tgtcggtaat aagatcgcca cggaagcgga tctgatggct
     6601 caactggtta agcggtcgtg aacggaagtt tatttcgggg aattgcgtca aataagttta
     6661 tttttgcgca atgctgggtt tgagccagca ttttttctat gaagagagca aaccgcaatg
     6721 aagagatagt aatgatccaa caaactgaaa atcaatcaac taaccaccga acgatccatt
     6781 cgcttacctc ggtccatccg gatgcccgtc tggctcctga cgtgagagtg gatcccttta
     6841 cgatgatcca taggaacgta gagatcgggg ccggaacgtg gataggcagt aacgtgacga
     6901 tcatggaagg tgccaggatc ggaaagaatt gcaggatctt ccccggcgcg gttgtatctg
     6961 ctgaccccca ggatctcaaa ttcgaaggcg aagagaccac cgtagagatc ggcgacaata
     7021 ccaccatccg cgaattcgtg accgtgcatc gcggcacgcg cgaccgctgg aggaccaaag
     7081 taggccggaa ttgtatgatc cttgcataca gccatatcgc gcatgactgt atcatcggcg
     7141 acaactgtat cctcagcaat aactcccagg tggcaggaca tgtgaccctt ggcgactggg
     7201 ttatcctcgg cggcatgtgt gctattcacc agtttgtaag agtcggttcg catgtcttcc
     7261 tcggcggcgg ctcgctcgtt gggaaagaca tcccccctta tatcaaggct gcccgcatgc
     7321 cgctcagcta tgccggagtc aattccgtgg gtctcaaacg ccgcggtttc accatcgaca
     7381 agatcaatca tatcctggat atctaccgtg tcatctacaa caagggtatg aataccagcc
     7441 aggcgctgga gttcctcgaa gaagagttcc ctgccagcga cgaacgggat gagatcgttg
     7501 ggtttatccg tgaaagcggc cgggggatca tcaaacgatt cagcaaaagc agtttcgatg
     7561 aagattacgc tgattgatgc cggtaaacgg ttcaaccgcg actggatctt tcgtcatctt
     7621 cactacgaat tcaacaccgg atgtaaatac gctatcaccg gccctaatgg ctcgggtaag
     7681 tcaaccttat tacaatccat tgccggcgcc atagccatca gcgaaggaac cattgactat
     7741 gcggcccctg ccgccaccgc aaaacccgcc tctgcaaagc cagcctccca agcctctgcc
     7801 accaccgcaa aacccgcaat ccccgcctcc accgccgttc cccccgacca ggcctaccgc
     7861 tatctttccc tcgccgcacc ctatctctca cccatagagg aaatgaccgt tactgaattc
     7921 cttgattttc ataagatctt caaacctttt ttacccaacc tcaccacccc cgacatcatt
     7981 cgcaccgtcg gcctggaaac cgcagcgcat aaacagatcc gtttctattc ctccggtatg
     8041 aaacagcg
//
LOCUS       DASYSV010000423         3380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1368007, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000423 DASYSV010000000
VERSION     DASYSV010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368007"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VGQ51_12975"
     CDS             complement(<1..1216)
                     /locus_tag="VGQ51_12975"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9037537.1"
                     /translation="MKRLLILAMVAMLATTPGQAQSYYFKSYQVNNGLSSNAITCSIQ
                     DHNGFMWFGSRNGLNRFDGNAFRIFRNRIGDTTSIGSNSILSLCETKSGLLWVGTTKG
                     VYLYDPSKETFHSFTQVPQGEVRYINEDRQGNIWIISNFNLYRYDPARQTARHFTASN
                     DETVCISISKTGRVWAATGNGIIKEYDAQKGDFISYNLVPLAKEEKLSRIQTLFSIND
                     SLILFGTLNHAYLLSIPTLSLRNIYKGQPALNDVQIHTIMQQSDTELWLGTERGLYVY
                     DLSTEKTILIQKKYQDPYSITDNVIFSFCKDREGNTWIGTFFGGIDYFSKEYNQFRKY
                     FPIPDANTLSGNLVHEICRDRYGDLFVGTEDAGLNKLNLKTGKWQHFLPGKSKEDISY
                     TNIHGLVADDDRL"
     gene            1405..>3380
                     /locus_tag="VGQ51_12980"
     CDS             1405..>3380
                     /locus_tag="VGQ51_12980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9037538.1"
                     /translation="MPVNRHCFRKLIFFTALSFFCIAGFGQDRVVSGKVLDKDNNMPV
                     SGASIRVKNSNGGVTADSNGDFSIRARPGSTLVVSSTGFGTLEYVPGGANAVIIRISR
                     SADALQDVVVVGYGTQRKKDITGSVASVSTKDFASLPVSDAGQAVEGKAAGVQVTASG
                     PPGNNVTFRVRGKGTINDADPLIVVDGVPTDAPLNNINPDDIASFDVLKDASASAIYG
                     SRGANGVILITTKRGHSGQGRLALNLMSGWQQPAKVVKMLDARQFAALNNEMLSNGGQ
                     AVNPAFGNPDTLGEGTDWIGALFHTAPMQKYTLSYSGGSAKDNYYVSASMLDQQGIVQ
                     TTAYKRYTIQFNNEARVLPWLKFGNNLTLTADDKPGMGANGATSYDIRNTLASNPIQP
                     IYNKDGTYSNVLGNPLWYGDITNQIGTAHINQNDTKSYDVLGNLYAEVTLLPGLTFKT
                     TGGLQAQFYNNRSWAPAYNFEPNPQTQSYLSQQSNTNLTYLWDNYLTYDHLFGSDHHL
                     TILAGTSAQNNRYSYISGNIVNFPSSVTQQLNSGTGAANVGGDGSEWAVLSFMGRVNY
                     SYKDKYLVTATLRRDGSSRFGPDNKYGTFPSASVAWRVSKESFFKDVSFINDLKLRAG
                     YGVTGNQNIGNYSFASNLNIAQYNFNNNIVSIVYP"
BASE COUNT          794 a          804 c          924 g          858 t
ORIGIN      
        1 acaaccggtc gtcgtcggcg accagcccat gaatattggt ataggagatg tcttctttgg
       61 atttaccggg gaggaaatgc tgccattttc cggttttcaa gttcaattta ttcaggccgg
      121 cgtcttccgt accgacaaaa agatcgccgt atctgtcacg gcatatttca tggacgaggt
      181 tgccgctaag ggtattggca tcgggtatgg gaaaatactt cctgaattga ttgtactctt
      241 tcgagaagta atcgataccg ccgaagaaag ttccgatcca ggtgttgccc tcacggtcct
      301 tgcaaaagga gaagatgaca ttgtcggtga tggaataggg gtcctgatat ttcttttgga
      361 tgaggattgt cttttcggtc gacaggtcat agacgtacag gcctctttcg gtgcccagcc
      421 acagttctgt gtcggactgt tgcatgatcg tatggatctg tacatcattc agggccggtt
      481 gccctttata gatgttccgg agggatagag ttgggatgct caggagatag gcgtggttca
      541 gggtgccgaa caggatcagc gagtcattaa tcgaaaagag cgtctggatc cgggaaagct
      601 tttcttcttt ggccagcgga accaggttgt aggatatgaa atcccctttt tgcgcatcgt
      661 attctttgat gatgccgttt ccggtggctg cccagacccg gccggttttg gagatagaga
      721 tacagactgt ctcatcgttt gatgctgtga aatgccgggc ggtctggcgg gcggggtcgt
      781 agcggtaaag attgaaattg gagatgatcc aaatatttcc ctgccggtct tcatttatgt
      841 acctcacctc tccttgcggc acttgtgtaa aggaatggaa ggtttctttg gaagggtcgt
      901 acaggtaaac accttttgtc gtgcctaccc ataataagcc gctttttgtc tcgcataagc
      961 tcaggatgga gttgctgccg atgctggtgg tatcgccgat ccggtttctg aagatcctga
     1021 aagcgttccc gtcaaaacga ttgagcccgt tgcggctccc gaaccacatg aacccattgt
     1081 ggtcctggat actgcaggtg atggcgttgc tggaaagccc gttattgacc tgatagcttt
     1141 tgaaataata ggattgggcc tgcccgggag tagttgcaag catcgcaacc atagccagta
     1201 taagtaatcg tttcactctt cgcgtctgtg tttattaacc cgggcaattt ataacttttc
     1261 aaagagatta aatgggtcat cgtggtcgga tgatcaaaat gagaacgttt gatcaaatat
     1321 tgatgctcac cgattgcacc cattgctgaa attgtgattc ttttacagcc gtaaaacgaa
     1381 aactaacgtt tcaacccgat tgttatgcca gttaatagac actgctttag aaaactgatt
     1441 ttttttaccg ctctttcttt tttctgtatc gccggttttg gccaggatag ggtcgtgtcc
     1501 ggcaaagtgc tggacaaaga caataatatg cccgtttcgg gcgcatcgat ccgggtaaag
     1561 aacagtaatg gcggtgttac ggctgattcc aatggtgatt tttccatccg ggcccggccg
     1621 ggttcgacgc ttgtggtaag ctcgaccggg ttcgggacgt tggaatatgt gcctggcggg
     1681 gcaaacgccg taatcatcag gatcagccgg tcggcggacg ccttacagga tgtcgtggtc
     1741 gttggatatg gaacccagcg gaaaaaggat attaccggtt cggtggcttc ggtcagcaca
     1801 aaggattttg cttctctccc ggtttccgat gccggacagg ccgtagaagg caaggctgcg
     1861 ggtgtccagg tgactgcttc gggccctccc gggaacaacg tgactttccg tgtcaggggc
     1921 aaggggacga tcaatgatgc cgatccgctg atcgtcgtgg atggagttcc taccgatgcg
     1981 ccattgaaca atatcaatcc ggacgacatt gccagttttg atgtgctgaa ggatgcatcc
     2041 gcgtcggcga tctacggatc gaggggtgcc aatggggtga tcctgatcac tacgaaacgc
     2101 ggtcattccg ggcaggggcg gctggcgttg aatttgatgt ccggctggca gcagccggcg
     2161 aaggttgtca agatgctcga tgcccggcag ttcgccgcct taaataatga gatgctgagc
     2221 aatggaggtc aggccgttaa tccggcattc gggaacccgg acaccctggg cgagggaacg
     2281 gactggatcg gcgccctttt ccatactgcc ccgatgcaaa aatatacgct gtcttattcg
     2341 ggcggatcgg caaaggacaa ctactatgtg tctgcttcca tgctcgacca gcagggcatc
     2401 gtacagacca ccgcctacaa acgctatacg atacagttca ataacgaggc cagggtactg
     2461 ccctggttga aattcgggaa caacctgacc ctgaccgcgg acgataagcc cgggatgggc
     2521 gcaaacggag ccacgtcgta tgacatcagg aacaccctgg cgtccaatcc gatccaacct
     2581 atttataaca aggacggaac gtattccaat gttttgggaa acccgttatg gtatggagat
     2641 attaccaacc agatcggcac ggcgcacatt aatcagaatg atacgaagag ctatgacgtc
     2701 ctggggaatc tctatgccga ggtgacgcta ttgccgggtt tgacttttaa aacgacgggc
     2761 ggactgcaag cgcaatttta caataaccgc agctgggctc ctgcctacaa tttcgagccc
     2821 aatccgcaaa cgcagtcgta tctgtcgcag caaagcaata cgaacctgac gtatttgtgg
     2881 gacaattatc tgacctatga tcatctgttc ggatcggatc atcacctgac aatccttgcg
     2941 ggaaccagcg cgcagaataa ccgctacagc tacatcagcg gtaatatcgt taattttccc
     3001 agcagtgtca cgcaacagct gaatagcgga acgggcgcag ccaatgtcgg cggcgacggg
     3061 tcggaatggg ctgtgctttc ttttatgggg cgggtcaatt attcgtacaa ggacaaatac
     3121 ctggtgacgg caacgctgag gcgggacgga tcttcccgtt ttggtcccga caacaagtat
     3181 ggcaccttcc catccgcctc ggtggcctgg agggtctcga aggagtcctt ttttaaggat
     3241 gtatctttca tcaacgacct gaaactccgg gccggctatg gcgtcacggg caatcaaaac
     3301 atcgggaatt attcctttgc ctcgaatctg aatatcgccc aatataattt caacaacaac
     3361 atagtttcca tcgtttatcc
//
LOCUS       DASYSV010000424         7356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_857553, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000424 DASYSV010000000
VERSION     DASYSV010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857553"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="VGQ51_12985"
     CDS             complement(<1..1532)
                     /locus_tag="VGQ51_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV9037539.1"
                     /translation="MPVFNQSRSNIIRLIFAGMFLIIAAQLFHLQIVSKKYKQQAQEN
                     ALFRKSVYPSRGIIYDRKGRAILNNTILYDLMVTPSQLKNIDTTYFCQLMGIDTAEFR
                     RRMGSYKIKSSPFRPVTFQDLLPPEVYARMEENIWKFPGFNLQERPVRVYPYNAAAHI
                     MGYVGEVDSAVIRRSNNYYQLGDYVGRSGLEQYYEKELMGERGIEFWIKDNKNRLVDH
                     YQGGTLDTPAIAGKNLHTYLDIELQQLAERLLFGKTGAVVALEPSTGGILAMASGPDF
                     DPNSLTGPDKQSNYAKLVLDASAPLFNRAIKGQYAAGSTYKPIGALIGLDEGVINPNS
                     GIDCRGFYYGCDRPVKCEEHNPGHAANLRLAIAHSCNSFFCNAFRLELDDPAIHSVRK
                     GLEKWKNYVNAFGLGHRLGVDLPSEDAGNIPDTTAYDKAYNHSWNSCTMVTMGIGQDK
                     MEVTPLQMANTMCIVANRGFYYTPHFVKSIEGAPEDDTLLSRFHQKHEPVTHIPDADY
                     DVV"
     gene            complement(1580..2113)
                     /locus_tag="VGQ51_12990"
     CDS             complement(1580..2113)
                     /locus_tag="VGQ51_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV9037540.1"
                     /translation="MSELLKNTVRFILFILVQVFILFKMPPLHRFIVPYLYYLYILWL
                     PFSLSRIGLTFVGFFFGLCLDYFTKTPGLHAAACTLIAYVRPFVIGLLISQEGADKNY
                     VAPSIVSMGWAPYATYVIVLTVLHHAYLVLLEWMNFGSLIYFLGKVIASSAVSLLLIL
                     LTELLFYRKQKFRTNTI"
     gene            complement(2110..2964)
                     /gene="mreC"
                     /locus_tag="VGQ51_12995"
     CDS             complement(2110..2964)
                     /gene="mreC"
                     /locus_tag="VGQ51_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV9037541.1"
                     /translation="MRNVFLFIRRHFNFLFFLVLQIIALSFLFRFNKFHEAAFLNVST
                     EITGRINERYNNVEYYFQLKKTNESLVQENLRLRQQLRENYEAPDSNKRLIHDTVRSD
                     TGQAYLKYNIMEAKVVNNTTLLPDNYLTIHRGFEQGVRANMGVVGPQGLVGSVINVSR
                     NFATVQSMLNSKFSEVAKLKNSNETGKVQWDGTNPQYVTMKNIPKSVKVEKGDTVVTT
                     QISSIFPANIMIGTIAEIIPDISSNFYTLKVKTATNFSTLEYVYVTDNLQYDEQKRLE
                     DSTRKKIQ"
     gene            complement(3012..4037)
                     /locus_tag="VGQ51_13000"
     CDS             complement(3012..4037)
                     /locus_tag="VGQ51_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEV9037542.1"
                     /translation="MGLFNFLTQEIAMDLGTANTLIIHNDEIVVNEPSIVALDRNNPK
                     NVLAVGKRALMMHEKTHESIRTVRPLKDGVIADFNAAELMIREMIKMIYPKKPLFPPS
                     WRMMICIPSSITEVEKRAVRDSAEQAGAKEVYLIHEPMAAALGIGIDVEEPVGNMIID
                     IGGGTTGITVIALAGIVCDQSIRIAGDEFTADIMEALRRYHSLLIGERTAEQIKIQIG
                     AALKDLDNPPDDIPVNGRDLVTGIPKQIMVSYQEIAEALDKSIFKIEEAILKALETTP
                     PELAGDIYRRGLYLTGGGALLRGLDKRLSQKIKLPVHIADDPLKSVVRGTGIALKNYD
                     RYPFVMR"
     gene            complement(4295..5275)
                     /locus_tag="VGQ51_13005"
     CDS             complement(4295..5275)
                     /locus_tag="VGQ51_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEV9037543.1"
                     /translation="MHYSDYLELDKILDAQHPESDRLGTPAHDEMLFIVIHQAYELWF
                     RQLLYETDSVIAIFGQPSLNDNSPELQTIVHRLSRCATILKVLVHQIDIMETMNPMDF
                     QDFRDLLRPASGFQSVQFRLLEARLGLQYEGRVGKQYYLSQLRPEEIDRVRAAETTPS
                     LLQLLNAWLERMPILDDTAGTAFWADYRGRYQSSLADAEKANLEAFDRVFFENSTDRT
                     LSPSACRSALFIMLYRGYPLLQLPYQVLHQLLEIDEQLSTWRYRHMSMVHRTIGTRIG
                     TGGSTGRDYLSVALEKHHLFREIAALTSFLIQRKRLPELSPEIRRRLGFG"
     gene            complement(5313..6626)
                     /gene="purD"
                     /locus_tag="VGQ51_13010"
     CDS             complement(5313..6626)
                     /gene="purD"
                     /locus_tag="VGQ51_13010"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV9037544.1"
                     /translation="MPLKFEFRAMKILLLGSGGREHALAWKLRQSNRCSALYIAPGNA
                     GTSQCGTNLPLSPLDFEGLAKFCTAENIGMVVVGPEEPLVNGIVDYFAAAETVRNIPV
                     IGPTAKGARLEGSKAFSKKFMQRHNIPTASYREFHSGNYADGITYIQQHPLPVVLKAD
                     GLAAGKGVIICQSTIEAVAEFELMIQGNKFGEAGAKVVVEEFLTGIELSIFVLTDGQH
                     FLVMPEAKDYKRVGEGDIGPNTGGMGAVSPVPFADAVLMDKITQRIIVPTVRGLAEEN
                     IDYKGFIFVGVIKVGDDPYVIEYNCRMGDPETEVVMPRLKNDLVELFQAVGEQRLDKM
                     TIQQEERTACTVVAVSGGYPGSYEKGLPISGLDGTPPVHSESLIFHAGTRAEKDAILT
                     NGGRVLCVTSFADTVYEAVDKSRDILENIYFDGIYYRRDIGFEFL"
     gene            6714..>7356
                     /locus_tag="VGQ51_13015"
     CDS             6714..>7356
                     /locus_tag="VGQ51_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEV9037545.1"
                     /translation="MSLTIAPKAKHPKALYVLALTEMWERFAYYLLAGILLLYLKDDQ
                     TGGKGIPESSAADITGTFLALIWLPLFVGGMIADRYLGYIRSIFIGGSLMAAGYLGLI
                     LPGNTTMYISLALIIIGNGFFKPNISTLLGNIYNKEELKPLKDNAYNIFYMGINTGAL
                     LCNFAAAYLRNKYTWGWAFGAAGIGMIIGLTWFGANLKHIRVGDVRKPPQKEDM"
BASE COUNT         1602 a         1842 c         2112 g         1800 t
ORIGIN      
        1 accacgtcat agtcggcatc ggggatatgc gtgacgggct catgtttttg atgaaaccgg
       61 ctgagaagag tatcgtcctc cggtgcccct tcgatgcttt ttacgaagtg cggcgtataa
      121 tagaagcccc ggttggcgac gatacacatc gtattggcca tttgcagcgg cgtcacttcc
      181 atcttgtcct ggccgatacc catcgtcacc atggtgcagg agttccagga atggttatag
      241 gccttgtcat aagccgtggt atccgggatg ttgccggcgt cctcgctggg caggtctacg
      301 cccaggcgat gcccgagacc gaaggcgttg acatagttct tccacttttc cagtcctttg
      361 cggacgctat ggattgccgg gtcgtcgagc tcgagccgga aggcgttgca gaaaaaggaa
      421 ttgcaggaat gggcgatggc cagccggaga ttcgcggcat ggcccgggtt atgttcttcg
      481 catttgacgg gacgatcaca tccatagtaa aagccgcgac agtcgatccc gctgttaggg
      541 ttgatgaccc cttcgtccag accgataagc gcgccgatcg gcttataggt ggaacctgcg
      601 gcgtattgac ctttgatcgc cctgttgaag agaggcgcgg aagcatcgag gaccagcttt
      661 gcgtagttgg attgtttatc gggtccggtc agcgaattgg ggtcgaagtc cggtcccgaa
      721 gccatggcca ggatgccgcc ggtggaaggc tcgagggcta cgaccgcacc ggtctttcca
      781 aaaagcaggc gttcggccag ctgttgcagt tctatatcca gataggtatg caggttctta
      841 ccggcgatgg cgggcgtgtc gagcgtgccc ccctgatagt gatccaccaa tctgttctta
      901 ttgtccttga tccagaattc gatacctcgt tcacccatca attccttttc atagtattgt
      961 tccagaccgc tgcggccgac atagtcgccc agctgataat aattattgct gcgccgtatc
     1021 acggcggaat ccacttcgcc gacataaccc atgatatggg cggcggcatt gtagggatag
     1081 accctgacgg ggcgttcctg gaggttgaag ccggggaatt tccagatatt ctcttccatc
     1141 cgggcataga cctcgggagg aagcaggtcc tggaaagtga cggggcggaa tggactgctc
     1201 ttgatcttat agctccccat acggcggcgg aattccgcgg tatcgatgcc catcagctgg
     1261 cagaaatagg tcgtatctat attcttaagc tgcgagggtg tgaccatcag atcgtacagg
     1321 atggtattgt tgaggatggc cctgcccttc cggtcgtaga tgatcccccg gctgggatag
     1381 acgcttttcc gaaagagcgc gttttcctgc gcctgctgtt tgtatttttt ggaaacgatc
     1441 tgcaggtgga agagttgagc cgcgatgatc aggaacatgc ctgcgaaaat gaggcggatg
     1501 atattactgc gcgattgatt gaatacaggc atatggattt gctcctacaa tgtacacatt
     1561 aaacgtctaa agtagccggt cagattgtat tggtcctgaa cttttgttta cggtagaaca
     1621 gcaattcggt gagcaggatc agtagcaggc tcacggccga ggaagcgatc acctttccga
     1681 ggaaatatat gaggctcccg aagttcatcc actccagaag cacgaggtaa gcgtggtgga
     1741 gcactgtcag gacgataaca taggtagcat atggcgccca acccatactc acgatgctcg
     1801 gagcgacata gttcttgtcg gctccttcct gcgagatgag gagtccgatg acgaagggac
     1861 gaacataagc gatgagggtg caggccgcgg catgaagacc cggagtcttg gtgaaataat
     1921 caagacaaag gccaaaaaag aaaccgacga atgtcaggcc gatccgggaa agactgaaag
     1981 gcagccaaag gatgtagaga taatacagat agggcactat gaaccggtgc agcggcggca
     2041 tcttgaagag gatgaacacc tgcaccagga taaagaggat gaaccggacc gtatttttca
     2101 gcaactcgct cattggatct ttttacgggt agagtcttcc agcctttttt gttcgtcata
     2161 ctgtagattg tcagtgacat atacgtattc aagggtggaa aagttcgtag ccgtcttaac
     2221 tttaagggta tagaaattcg aggagatgtc gggtatgatc tcggcgatcg taccgatcat
     2281 gatgttggcc ggaaagatcg aggagatctg ggtggtcacg acggtatctc ccttttcgac
     2341 cttcacgctt ttgggaatat tcttcatcgt gacatattgt gggttagtgc cgtcccattg
     2401 gacctttccc gtctcgttgc tgttcttgag cttggccacc tcgctgaact tggagttgag
     2461 catgctttgg acggtggcga aattcctgct cacattgatc acggaaccta ccagtccctg
     2521 gggtccgaca acgcccatat tggcacgcac gccctgttcg aatccgcggt gaatggtcag
     2581 atagttgtcc gggagcaacg tggtgttgtt cacgaccttc gcctccatga tattatattt
     2641 caggtaggcc tgacccgtat cggaccttac cgtatcatgg ataagccgtt tgttggaatc
     2701 cggcgcctcg tagttctccc tgagctgttg cctgaggcgg aggttttcct gtacgagcga
     2761 ctcgttcgtt ttcttcagtt ggaaataata ttcgacattg ttgtaacgct cgttgatgcg
     2821 tccggtgatc tcggtgctga cattgagaaa ggctgcctcg tggaatttgt tgaaacgaaa
     2881 caggaaggac agggcgatga tctgcaacac caggaagaag aggaagttga aatgccttct
     2941 gatgaagaga aaaacgttac gcaccggtgt gtagtttctg ttggtgatct gttaatggcg
     3001 gctggctcgt tctatctcat gacgaacgga taccggtcat aattcttcag cgctatacct
     3061 gtccctctga ccacgctttt cagcgggtcg tcggcgatat gcacgggtag ttttattttc
     3121 tggctcagcc gtttgtcaag gcctcgcagc agcgctccgc cacccgtcag gtagagaccg
     3181 cggcggtaga tatcgccggc cagctcggga ggcgtcgttt ccagcgcctt gaggatggct
     3241 tcttcgatct tgaagatgct tttatcgagg gcttcggcta tttcctggta ggataccatg
     3301 atctgtttgg ggataccggt gacgaggtcg cggccattga cggggatatc gtcgggcggg
     3361 ttgtccaggt ctttgagtgc ggcgccgatc tggatcttga tctgctcggc ggtacgttca
     3421 ccgataagca gagagtgata ccggcgaagg gcttccatga tatcggcggt gaattcatcg
     3481 ccggcgatac ggatcgattg gtcgcacacg atgccggcga gcgcgatgac ggtgataccc
     3541 gtggtaccgc cgccgatgtc gatgatcatg ttaccgacgg gttcttccac gtcgatgccg
     3601 atacccagcg cggctgccat aggctcatgg ataagataga cctcttttgc gcctgcctgt
     3661 tcggcgctgt cccgtacggc tcttttctcg acttcggtaa tgcttgaggg gatacagatc
     3721 atcattcgcc agctgggcgg aaaaagaggt tttttgggat agatcatttt gatcatttcc
     3781 cgtatcatga gctcggccgc gttgaagtcg gcgatgaccc cgtctttcag ggggcggacc
     3841 gtccgtatgc tttcatgggt tttctcgtgc atcatcagcg cgcgtttgcc gacggccagc
     3901 acgttcttgg ggttatttcg atccagggca acgatgctcg gttcattcac tacaatctcg
     3961 tcgttatgta tgatcagggt attagccgta ccgaggtcca tggctatttc ttgcgtaagg
     4021 aaattgaata aacccatttg atttaaaagg ttcgaatttt tttagaattt aaccggccac
     4081 cgttcccagc tcggcctgct tgtaaattgt cagcaaaatt gaaggaaata attcgaatta
     4141 acaaggtgaa acccctgata tgtggattat ttccataggt attggctgaa aatcaataac
     4201 gacaaaagcg gtagcatcgt atctgcgatg gggataaaaa catccgcgcg cacacagcga
     4261 cccggccatg cagccgtgtg gcggcatata gagatcaacc aaacccaagc cggcgccgga
     4321 tctccggcga caattccggt aaccttttcc gttggatcag gaagctggtc agtgccgcga
     4381 tctcgcgaaa gaggtgatgc ttttcgaggg ctactgacag gtagtccctt ccggtgctgc
     4441 cgccggtgcc gatgcgcgtt ccgatggtgc gatgtaccat gctcatatga cgatatcgcc
     4501 aggtgctgag ctgttcgtca atttccagca gctggtgtag tacctggtag ggcagctgga
     4561 gcagcggata accgcgatag agcattatga aaagcgccga gcggcaggcg gacggggaga
     4621 gggttctatc ggtagagttt tcgaagaaaa ccctgtcaaa ggcctccagg tttgcttttt
     4681 ccgcatcggc caggctcgac tgatagcgcc cgcggtagtc ggcccagaag gcggtcccgg
     4741 cagtatcgtc gaggatgggc atacgttcga gccaggcgtt cagcagctgg aggagtgaag
     4801 gggtcgtttc ggcggccctt acccgatcga tctcttcggg ccgcagctgt gaaagataat
     4861 attgtttccc gactcgccct tcgtattgga ggcccagcct ggcttccagc agccggaact
     4921 gcacgctttg gaaaccggag gctggtcgga ggagatcgcg gaaatcctgg aaatccatgg
     4981 gattcatggt ttccataata tctatctgat gtacgagcac cttgaggatg gtggcacagc
     5041 gtgacaaccg gtggacgatg gtctgcagtt cgggtgaatt atcgttgagc gagggctgcc
     5101 cgaagatagc gatgacggag tccgtctcgt aaagcagctg gcggaaccat agctcgtagg
     5161 cctggtggat gacgatgaag agcatttcgt catgggcagg ggtgcccagg cggtcgcttt
     5221 cgggatgctg cgcatccagt atcttatcca attcgagata atcgctgtag tgcatggctc
     5281 ggttgtggcc ggcggagctg tgctccgcct agttatagaa attcgaatcc gatatcgcgg
     5341 cgataataaa tgccgtcgaa ataaatattt tcgaggatat ccctggattt gtctaccgcc
     5401 tcatagacgg tgtcggcgaa ggaggtgaca cagagtaccc ggccgccatt ggtgagaatg
     5461 gcatcttttt cggcccgggt tccggcatgg aagatcaggc tttcggaatg gaccggcggt
     5521 gtaccatcga gaccgctgat gggcagtcct ttttcatagg agccgggata gcccccgctg
     5581 acggcgacga ccgtacaggc agttcgttct tcctgttgga tggtcatctt atcaagtctt
     5641 tgctcgccaa cagcctggaa aagctcgacc aggtcgtttt taagccgggg catgaccact
     5701 tccgtttccg ggtcgcccat gcggcaatta tattcgatga catagggatc gtccccgacc
     5761 tttatcaccc cgacgaaaat gaatccttta taatctatgt tctcttccgc aagcccccgg
     5821 acggtaggga cgatgatccg ttgggtgatc ttatccatta agacggcatc ggcgaacggc
     5881 accgggctca cggcgcccat cccccccgta ttggggccaa tatcgccttc ccctacccgc
     5941 ttgtaatcct tggcctccgg cattacgagg aagtgctgcc cgtccgtcag tacgaaaatg
     6001 gaaagctcga tcccggtgag gaattcctcc accactactt tcgcacctgc ttccccgaat
     6061 ttgttaccct ggatcatgag ctcgaactcc gccacggctt cgatagtgct ttggcagatg
     6121 atcacaccct ttccggccgc gaggccatcc gccttaagga ctaccggcag ggggtgttgc
     6181 tggatatagg tgatgccgtc ggcataattc ccggagtgga actcgcggta ggaggcggta
     6241 gggatattat ggcgttgcat gaattttttt gaaaaggcct tgctgccttc cagccgggcg
     6301 cctttggccg tgggaccgat aacgggtata ttccggacgg tctcggcggc tgcgaaataa
     6361 tcgacgatgc cattgaccag gggttcctcg ggtcctacga ccaccattcc tatgttctcc
     6421 gcggtacaga atttagcaag tccttcgaaa tccagtggtg aaaggggcag attggtgccg
     6481 cattgggagg tgccggcatt acccggtgcg atgtacagcg ccgaacagcg gttgctttgt
     6541 ctgagtttcc aggcaagcgc atgctcccgg ccgccggagc ctaacaagag gatcttcatg
     6601 gcccgaaatt cgaatttcag gggcatatag taaaaaagaa tttactagtg gtcattatcc
     6661 ggggagggtc caacgggata tttgcttatt ttgggcccct aattcctctg cgcatgagtc
     6721 tgacgattgc cccaaaagcc aaacatccga aagccctgta tgtccttgca ctcacagaga
     6781 tgtgggaacg atttgcatac tacctgcttg ccggcattct cttattgtat ctaaaggacg
     6841 atcaaacggg cggcaaaggt atcccggaaa gcagcgccgc cgacatcacg ggtaccttcc
     6901 tggcgctgat ctggctaccg ctcttcgtcg gcggaatgat cgccgaccgc tatctcggct
     6961 atatccggtc catttttatc ggcggatcac tgatggccgc cggttacctg gggttgatcc
     7021 tgccgggaaa caccacgatg tatatctcgc tggccctgat cattatcggg aatgggtttt
     7081 tcaaacccaa tatcagcacc ctgctcggga acatctataa taaggaggag ctcaagccgc
     7141 taaaagacaa tgcctacaat atcttctata tgggcatcaa cacaggtgcg ttgctgtgca
     7201 attttgcggc ggcatatctc cggaataaat acacctgggg ttgggccttc ggtgcggcag
     7261 gcatcggcat gatcatcggt ctgacctggt ttggcgccaa tctgaagcat atccgggtgg
     7321 gggacgttcg caagccgccc caaaaagagg atatgg
//
LOCUS       DASYSV010000425         6621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2009643, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000425 DASYSV010000000
VERSION     DASYSV010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009643"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1911)
                     /locus_tag="VGQ51_13020"
     CDS             complement(<1..1911)
                     /locus_tag="VGQ51_13020"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEV9037546.1"
                     /translation="MITGPTTVCVNSPVALSNGSAPLPPTTVWDFGDGTAANSPNPSK
                     TWDAIGSYVVKMVNRYAVCADSTTQTVQVVTSPTPTFTAAPNTGCKAPLTVQFTNTTP
                     GATSSSWDFGDGNTSTATNPTHTYTSAGNFDVTLTTTSGSNCSGTSTQTAFIKIAPPS
                     VTIRNAPNLGACVQGSGGAANSLNPSAIVNSVNNTFTLNWTATGSNEGSSNADFPTFT
                     YNTAGTYSISVTATTSDGCTSAPATSPVQIGTPVLPASFSFSPPVICGRSPVTFTSTA
                     SGGDHWLWNFGDGTTSGAVNSSTATHIYNNKISPPSLMVSLTITNNGCPTTSSQSITI
                     NPPIPNFGYKVSCPSTVTFTDSSLTDATPTSYTWDFGDGSPAQVVNTAPWLPTAHTYA
                     PGTYTAKLTVADGATCPPQSWPKTITIAAVTASFTYTPSPTVCVNGPFTLTSTSTVNP
                     DPPGTSYISSYTWQINGLPAARGPHDYTTSLPTPGAYNVVLIDTDQNNCPHVSPPQTI
                     QVTGPTPKFAVLPNGGGCVNSPVIFTDQSTPWPGPPPNTIVKWAWDFADGNTQESITA
                     PMSHSYTDTGTYKVILTATDDKGCFASDTVPVLVATPFANFASPDSFYCANEPLTFID
                     SSDGYGITDSWNF"
     gene            complement(2281..2757)
                     /locus_tag="VGQ51_13025"
     CDS             complement(2281..2757)
                     /locus_tag="VGQ51_13025"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEV9037547.1"
                     /translation="MRIRLLIASCCLCLLGFSHARAQAPGTDFSANPTAGCGPLTVQF
                     KDISTNGPLYWSWDFGNGITSSIQNPTATYSTPGTYTVTLIARNKNGSNAMRKTDYIT
                     VYPFPIPKFTSNLTLACAPAGVQFLDQSTPGQGGITNWAWDFGDGTTSNSQNPSHT"
     gene            complement(2951..3373)
                     /locus_tag="VGQ51_13030"
     CDS             complement(2951..3373)
                     /locus_tag="VGQ51_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037548.1"
                     /translation="MKLLYRTTLFILLVASVLSCKKYVQQQEQNAALSIMTNGLWYVS
                     GYKQATTDITASFSGYLFKFDANNTVTGTLGNSSTTGQWSDDLVARTITADFPGAGQP
                     LVNLNATWRITDSYVDSVSARYTDTVNHVTDYLQLKKQ"
     gene            complement(3392..4903)
                     /locus_tag="VGQ51_13035"
     CDS             complement(3392..4903)
                     /locus_tag="VGQ51_13035"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEV9037549.1"
                     /translation="MPLTNTTEDLYSTYTDRMQKIADVKYSAAVLQWDQETYLPTKGA
                     DFRGRQLATLSEIAHEWFISPDLGQLLNELHSRGDLPEDRRRNIELSLEDYEKQKKFT
                     PAFIRRLSEASSRCFHSWLQARAANSFSFYEDDLATMVQLKKEEADLAGYKQHPYDAL
                     LDQYEKGATTTLLDKVFSEVRTPLKDLLDRIGSRPQVDDSFLRGNFPRQKQWDFGMDI
                     IRQLGFDFDAGRQDISEHPFTTSFNPRDVRITTRIDEKDFSHMTWSCIHETGHALYEQ
                     GIPTEYYGLPLGEFASLGIHESQSRLWENNVGRSLGWWRFFYPRLQSIFPSRFKDVTT
                     EQFYHGINKVQPSLIRTEADEVSYHFHVMIRYELEKQLIEGSLPVRDIPAWWNDNYKT
                     WLGVTVPDDKHGCLQDVHWSHGSFGYFPTYSLGSFYAAQFFSAAENQLPNLSASIDAG
                     NTAPLLDWLRTNIHRWGRQFTSEELCRKATGNPLEIVYFLDYLHNKYVNIYGF"
     gene            5102..>6621
                     /locus_tag="VGQ51_13040"
     CDS             5102..>6621
                     /locus_tag="VGQ51_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037550.1"
                     /translation="MKKFKSFFLIILAMMPFLLKAQMATQTSAQKVDSMLGIYADNYP
                     QEKIYVQFDKKIYNAGETIWFKAYVFTGADPSLMSRNFYAELSDPNGVVLQRKVYPIG
                     ESSTAGNFDLPKDVKSRHLHFRAYTGWMSNFDTAFYFEKDIRIYDKKLDSNTTVTLIP
                     RQDHLQFFAEGGDMVAGVENMVAFKCNDQFGLPVPVKGKVQDKSGKDVLTFASEHDGM
                     GKFVVTPDKDDSLTAVWLDEKGIEHRTGLPEVKQIGVVLRAIPGKQKVLFSVARSAEG
                     GPDFKHLTIIASMHQHLVYKARINLEDNFMSGGTIPTAQLPSGVLVMTVLTQQMVPVS
                     ERVVFVNNNEYLFKPELEIALKGVVRRGKNSLTIDVPDTLKSNLSLAVTDATADGEFP
                     DDDNIVSRLLLTGEVKGYVHDPYYYFSGTSDSIAGQLDLVMLTHGWRRIKWESLVRGQ
                     LPVIKNPEQDYLSIRADVLGIDPYKIAKDELINVILSKKDSSTQMLQVPHLSGTKFG"
BASE COUNT         1445 a         1422 c         2194 g         1560 t
ORIGIN      
        1 gaaattccag gaatcggtga tgccgtatcc gtccgaggag tcgatgaatg tgagaggttc
       61 gttggcgcaa tagaagctgt cgggtgatgc aaaattcgca aagggggtcg ccacgaggac
      121 ggggaccgta tcggaagcaa agcaaccttt gtcatcggtg gccgtgagta tgaccttata
      181 agtgcctgtg tcggtatagg aatgcgacat gggtgcggtg atggactcct gcgtgttgcc
      241 gtcggcgaaa tcccaggccc attttacgat cgtgttgggc ggtggaccgg gccagggtgt
      301 ggattggtcc gtgaagatga cggggctgtt gacacagccg ccgccgttgg gaaggacggc
      361 gaatttggga gtgggcccgg tgacctggat ggtctggggc ggtgaaacat gggggcaatt
      421 gttttggtcc gtgtcgatga ggacgacgtt ataagcgccc ggagtgggta ggcttgtcgt
      481 atagtcgtgt gggcctctgg ctgcaggcag gccgttgatc tgccaggtat aggagctgat
      541 ataggaggtg cccggcggat cggggttgac ggtactggtg gaagtgagcg taaaggggcc
      601 attgacgcag actgtcgggg acggggtata ggtgaaagat gccgtgaccg cggcgatcgt
      661 aatggtcttg ggccatgatt ggggcgggca ggtggcgcca tcggctacgg tgagcttggc
      721 ggtataagtg cccggcgcat aggtatgcgc ggtgggcagc cagggtgcgg tgttaactac
      781 ctgtgcggga ctgccgtcgc cgaagtccca ggtgtatgat gtgggggtgg cgtcggtgag
      841 cgaggagtcc gtgaaagtta cggtactggg gcagctgacc ttataaccga agttggggat
      901 gggtggattg atcgtaatgc tttgggagct tgtggtcggg catccgttgt tcgttatggt
      961 aagggagacc attagcgagg gcggactgat cttattgttg tagatgtggg tcgcggtgga
     1021 cgagtttacg gcgcccgagg tggtgccgtc accgaaattc cagagccaat gatcacctcc
     1081 tgaagccgtc gacgtgaacg tgaccggact gcgaccgcag atgacgggcg ggctgaaaga
     1141 aaagctcgca gggaggactg gcgtgccgat ctggaccggg ctggtggcgg gcgcggaggt
     1201 gcagccatcg gaggtagtgg cggtgacgct gatgctatat gtgccggccg tgttgtaggt
     1261 gaaggtcggg aaatcggcat tcgaactgcc ttcgttggag cctgtggctg tccagttgag
     1321 cgtaaaggtg ttattgacgg aattgacgat ggcgctgggg ttaagcgaat tggcggcgcc
     1381 gccgctgccc tgcacgcagg ctcccaaatt gggcgcgttg cggatggtga cggagggagg
     1441 cgctatttta atgaaagcag tctgggtgct cgtaccggaa caattgctgc cgctggtagt
     1501 ggtaagggtt acatcgaaat taccggcgct tgtataggta tgtgtcgggt ttgtcgcggt
     1561 agaggtattg ccatcgccga aatcccagga cgaggacgtg gcgccggggg tcgtattggt
     1621 gaactgaacg gtcagcgggg ccttacagcc ggtgttcggg gcggcggtaa aggtcggagt
     1681 gggagaagtg acgacctgga cggtctgggt cgttgagtcg gcgcagacgg cgtatcggtt
     1741 gaccattttt acgacatagg agccgatggc gtcccatgtt ttggatggat tgggactgtt
     1801 ggcggcggta ccatcgccga aatcccagac ggtagtcggg ggcagcggtg ccgacccgtt
     1861 ggataatgcg acgggcgaat tgacgcagac agtcgtgggt cccgtgatca ccgcgttatt
     1921 gccgctgagc ggcagggtat cctgtattgt cgcgctacag ccgagactgc tgctgacctg
     1981 cagcgcaacg atataggtgc cgttggcgga ataggtgaca ttggaggggt tggtagcggt
     2041 ggaattggac ggtatggccc cggcgcccag gtcccagttg taactaaggt tccccggccc
     2101 ggaggtttga ttgatgaaat tgatcgtgta gggggcggta caggcattgg agatctggtt
     2161 ccaggtgaaa ttgggttgga tgccctggac gatacgaata taacggccga cggcagtcga
     2221 attgacgcag cccccggaat tggttatttt cagcgtaacg gtatagtaac ctgtttgggt
     2281 ctaggtgtgt gacggatttt gagaattgga ggtagttccg tcgccgaaat cccaggccca
     2341 gttagtgatc ccgccctggc cgggggtaga ctggtctaag aattggaccc cggcgggagc
     2401 gcaggccagg gtcaggttcg aagtaaattt tggtatcgga aaggggtaga cggtgatata
     2461 gtcggtcttg cgcatcgcgt tagagccgtt tttgtttcgg gcgatgaggg tgaccgtata
     2521 ggtgccggga gtggaatagg tagcggtggg gttttggata gaggaggtga tgccgttgcc
     2581 gaagtcccag ctccaataga gggggccatt ggtggatata tccttgaatt gaacggtaag
     2641 agggccgcat ccggcagtag ggttagcgga aaagtctgtg ccgggtgcct gggcccgggc
     2701 gtgggaaaag ccgagaagac agaggcagca ggatgcaatg agtagtctta ttctcaacga
     2761 aacgttcgtt taggtatgcg attaactagc aaatggcgga ccggtttcgc agggtattgg
     2821 attgtaggac tttccgcagg gattggatcg ggacgacttg cagggaaccg gatcgggacg
     2881 actttccgca gggattggat ttaggcgact tgcaaggaat cggatgggga cgacttgcag
     2941 ggaatcggat ctattgtttc ttcagctgca ggtaatctgt aacatggttc acggtatccg
     3001 tatacctggc ggaaacgctg tctacatagc tatcggtgat cctccaggtg gcgttcagat
     3061 ttaccagggg ctgaccggct cccgggaaat ccgccgtgat cgtacgggcg accaggtcgt
     3121 ccgaccattg acccgtggtc gacgagttgc ccaaggtgcc cgtgacggtg ttatttgcgt
     3181 caaatttaaa gagataacct gaaaaagaag cagttatgtc ggtggtggct tgcttgtatc
     3241 cactgacgta ccaaagtccg ttggtcatga tggacaatgc tgcattttgt tcctgctgct
     3301 ggacgtactt cttgcaggaa aggacggatg ccacaagcag gatgaacagg gttgtacgat
     3361 ataaaagctt cataggctat ggattatgtt atcaaaatcc gtatatatta acgtatttat
     3421 tgtgtaaata gtctaagaaa tatacgattt cgagggggtt cccggttgct ttccggcaaa
     3481 gttcttcgct ggtaaattgg cggccccagc gatggatatt cgttcttagc cagtcgagca
     3541 ggggggcggt attcccggca tcgatggagg cgctaaggtt aggtaattga ttctcagcgg
     3601 ctgagaagaa ttgagcggca taaaagctgc ccagactata tgttgggaaa tagccgaagc
     3661 tgccatgact ccaatggacg tcctggagac aaccgtgttt gtcatcgggg acggtgacgc
     3721 ccagccaggt tttataattg tcgttccacc aggcagggat atccctgacg ggaaggctgc
     3781 cttcgatcag ctgtttttcc aattcatagc ggatcataac atgaaaatga taggaaacct
     3841 catcggcctc ggtgcggatg agcgaaggct ggaccttgtt gatcccgtgg tagaattgtt
     3901 ctgtagtgac gtctttgaac cgggaaggga aaatggactg gagacgggga tagaagaagc
     3961 gccaccaacc gagactgcgt cctacgttgt tttcccaaag tctcgactgt gattcgtgga
     4021 tgccgagcga agcgaattct cccaggggga gaccgtagta ttccgtaggg ataccctgtt
     4081 catacagcgc gtggccggtc tcgtgaatac agctccaggt catgtggctg aaatcctttt
     4141 cgtcgatgcg ggtggtgatc cgcacatcgc gcgggttgaa gctggtagtg aacgggtgtt
     4201 cggagatgtc ctggcggccg gcgtcgaagt cgaagcccag ctgccggatg atatccattc
     4261 cgaaatccca ttgtttttgt cggggaaaat tgccgcgaag aaagctgtcg tctacctgcg
     4321 ggcggcttcc gatgcggtca agcagatctt tgagaggggt gcggacttcc gagaagacct
     4381 tgtccagaag ggtggtggtc gccccttttt catactgatc cagcaaggcg tcgtaaggat
     4441 gttgtttgta gcccgccaaa tccgcctctt cttttttcaa ctgcaccatc gtggccagat
     4501 cgtcttcata gaacgagaag gaattggctg cgcgggcctg cagccaggag tggaaacaac
     4561 gggagctggc ttcgctgaga cggcgaatga aagcgggcgt gaatttcttt tgtttttcgt
     4621 agtcttcgag acttagttct atattgcgac ggcggtcttc ggggaggtcg ccacgagaat
     4681 gcaactcgtt aagaagctgg ccgaggtccg gggagatgaa ccactcgtga gcgatctccg
     4741 aaagtgttgc gagctggcgg ccgcgaaaat ccgcgccttt tgtcggcaga taggtttcct
     4801 ggtcccattg caaaacggct gccgagtatt tgacatcggc tattttttgc atacgatcgg
     4861 tataggtcga gtaaagatct tcagttgtat ttgttaatgg cattttaaca ttttttattg
     4921 caaaacattc cgaaattaag cgcggagccg ccggtggcgg ctcgcccacg gccgagcgaa
     4981 gcgaaggccg cgaccgggga acgcgccggt ggtgcgggcc gaaggtcggc agacattagc
     5041 gcattgccga aaatacgcta gtttttggct gaagcgtatt ttcggcacat atttgtagag
     5101 tatgaagaaa ttcaagagtt tttttctgat tattttggcg atgatgcctt ttttgttgaa
     5161 ggcgcagatg gcgacgcaga cgagcgcgca gaaggtggac tcgatgctgg ggatctatgc
     5221 cgacaattat ccgcaggaga agatctatgt tcaattcgac aagaagatct ataacgccgg
     5281 cgagacgatc tggttcaagg cgtatgtctt taccggcgcc gatccgtcat tgatgagcag
     5341 gaacttttat gcggagcttt cggatccgaa tggagtggtc ctgcagcgga aggtttatcc
     5401 tataggcgaa tcgtcgacgg caggtaattt cgatctgccc aaagacgtga aatcacggca
     5461 tcttcacttc cgggcttata cgggttggat gtcgaatttc gatactgcat tctatttcga
     5521 gaaggatatc cggatctatg acaagaagct ggactcgaat accaccgtga cattgatacc
     5581 ccgccaggat catcttcaat tttttgccga aggtggagac atggttgccg gtgtggagaa
     5641 tatggtcgcc ttcaagtgca atgatcaatt cggattacca gtgcctgtaa aggggaaagt
     5701 gcaggacaag agcggcaagg acgtgctgac gtttgcatcg gaacatgacg gcatggggaa
     5761 attcgtagtg acgcccgata aggatgattc gctgacggct gtgtggctgg atgagaaggg
     5821 gatcgagcat cggaccggtc ttccggaggt aaagcagatc ggggtggtcc tgagagcgat
     5881 acccggcaag caaaaggtac tgttttcggt ggcgcggagc gctgagggcg gaccggattt
     5941 caagcatctt actatcattg cgagtatgca tcagcacctg gtctacaagg cccggatcaa
     6001 ccttgaggat aatttcatga gcggcggaac gatccctacc gcacagcttc ccagcggggt
     6061 cctggtcatg acagtcctca cccagcaaat ggtgcctgtt tccgaaaggg tggtatttgt
     6121 gaacaacaat gaatacctgt tcaagcctga attggagatc gcattaaagg gcgtggtccg
     6181 gcggggcaag aacagcctga ccatcgacgt gccggataca ttgaaaagca atttatcgct
     6241 tgcggtgacg gatgccacgg cggatgggga gttcccggat gacgacaata tcgtgtcccg
     6301 gttgttgctg acgggtgagg tgaagggtta tgtccacgac ccttattatt atttttcagg
     6361 tacttcggat agcattgccg ggcagctgga tctcgtcatg ctcacgcatg ggtggcggcg
     6421 tatcaaatgg gagtcgctgg tgcgggggca attgccggtg atcaaaaatc cggagcagga
     6481 ttatctttcg ataagagcgg atgtactggg gatcgatccc tacaagatag ccaaggatga
     6541 attgatcaat gtgatcctga gtaaaaaaga ttcgagcacg cagatgcttc aggtgcctca
     6601 tttgtcgggg acgaaattcg g
//
LOCUS       DASYSV010000426         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2390136, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000426 DASYSV010000000
VERSION     DASYSV010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390136"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VGQ51_13045"
     CDS             <1..929
                     /locus_tag="VGQ51_13045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV9037551.1"
                     /translation="FCECFLYPLFLHRYYGSGTHRIVSAWPDGIPAAITARMLPFRSR
                     MRLGAWLHVFLPAMISRRTNKDAPLPAFSKTRLLHLLSNLEDVVRKLDSRAFRDPGWS
                     DYYEKSILSKTYLDEKQALFTSMIGGIDFSSALDLGANNGHFSRILAEKGIPVIAAEA
                     DWQCVETLYRAGGSESHPIQALCVDIANPTPATGFDHRERSSFSDRAVSDLVVALALI
                     HHLVLGRNIPLPMVARYLARLTRRWLLIEFVPPGDERSMQLVRNKSAYPTPYDEAEFE
                     KQFSAFFTIDDKAPVPGTRRVLYRMRKIPIFA"
     gene            943..1914
                     /gene="bioB"
                     /locus_tag="VGQ51_13050"
     CDS             943..1914
                     /gene="bioB"
                     /locus_tag="VGQ51_13050"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEV9037552.1"
                     /translation="MKHNKWTLQEIEAIYNSPLLELIYRAASVHRQYQDTAEVQVCTL
                     LSIKTGGCPEDCAYCPQAARYNTGVDVQALMKKEEVLEYAAKAKAAGSTRFCMGAAWR
                     EVRDNKDFDRVLDMVKGVNQMGMEVCCTLGMLNEDQARRLADAGLHAYNHNLDTSSEY
                     YTEIISTRTYDDRLTTLDNVRKAGVTVCCGGIIGLGETHADRIGMLHTLATLPQHPES
                     VPVNALVRVKGTPLENNPKVDVWDMIRMIATARIIMPSTMVRLSAGRSEMSQAEQALC
                     FMAGANSIFAGEKLLTTPNPSFEEDNAMFQLLGLKPRMAFKEENQPA"
     gene            complement(1926..2342)
                     /locus_tag="VGQ51_13055"
     CDS             complement(1926..2342)
                     /locus_tag="VGQ51_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9037553.1"
                     /translation="MIRLVIVDDHMHVREAWSWVLSQVPGLNVIAQCANGLEAIEAAR
                     QLQPDVMLMDIHMNPVNGLEATRTIRGFAPDIKIIGVSVQAERSYVTEMLRSGANGYV
                     TKNSPSEEMVTAIDEVLAGKTYICEEVGQMRPVARF"
     gene            complement(2549..>3257)
                     /locus_tag="VGQ51_13060"
     CDS             complement(2549..>3257)
                     /locus_tag="VGQ51_13060"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEV9037554.1"
                     /translation="ESGVRELDRQLASIMRWLAKEYAMKGKLKAQLTQTDIDKILGRA
                     RYSNDLYKVANMAGVAVGLAWTYVGGDILFVETSLSDGKGDLKLTGNLGNVMKESAAT
                     ALTYLQSNARKYDIDPDIFNKKTIHVHVPEGAVPKDGPSAGVTMMSSIASAFTGRRVK
                     PYLAMTGEITLRGQVLPVGGIKEKILAARRAGLKEVVLCWQNEKDVQEIDSSFIRGLK
                     FNFVKTMQQVLELSLVQ"
BASE COUNT          756 a          975 c          807 g          719 t
ORIGIN      
        1 agttctgtga atgttttcta tacccgctgt tccttcatcg ctattatgga tcgggaacgc
       61 accgcatcgt cagcgcctgg cccgacggca tcccggcagc catcaccgca cggatgctgc
      121 ccttccgcag ccggatgaga ctgggagcgt ggttgcacgt ctttctaccg gcaatgatca
      181 gccgacggac taataaggat gctccattgc ctgctttcag caagacccgg ctcctccatt
      241 tgttatccaa tctcgaagat gtcgtccgca agctggattc ccgcgcattc cgtgacccgg
      301 gatggagcga ttactatgag aaaagcattc tgagcaagac ctatctcgat gaaaagcaag
      361 cgctcttcac ctcgatgatc gggggcatcg atttttcctc cgccctggac ctgggagcca
      421 ataacggcca cttcagcagg atcctcgccg aaaaaggcat tccggtcatc gccgccgagg
      481 cggattggca atgcgtcgaa accctttatc gggccggcgg cagcgaaagc cacccgatcc
      541 aggccctttg cgtggacatc gccaacccta ctcccgccac cggtttcgat caccgcgaaa
      601 gaagctcctt ttccgacagg gcggtcagcg acctcgtcgt ggcgctcgca ctgatccatc
      661 acctcgtcct tggacgcaat atccccctgc cgatggttgc ccgctatctg gcccggctga
      721 cccgccgatg gctgcttatc gagttcgtcc cgcccggcga cgaaagatcc atgcagctgg
      781 tgcggaataa aagcgcttat ccgacaccgt acgacgaggc cgaattcgaa aaacaattct
      841 cggctttttt taccatcgac gacaaggctc ctgttcccgg aacccggcgg gtcctttacc
      901 ggatgagaaa aattccgata tttgcctgat aatatgaatc caatgaagca caacaaatgg
      961 acgctgcagg aaattgaagc catctataac agccctttgc tcgagctgat ctaccgtgcc
     1021 gcctccgtac accgccaata ccaggacacc gcggaagtgc aggtttgcac gctgctatcc
     1081 ataaagacag gcggatgccc cgaagactgc gcttactgcc ctcaggcggc ccgctataat
     1141 acgggcgtcg atgtacaggc gctgatgaag aaagaagagg tgctggaata tgccgccaaa
     1201 gcgaaggcgg cgggttcgac ccggttctgc atgggagccg cctggcgtga ggtcagggac
     1261 aacaaggatt tcgaccgcgt tttggacatg gtgaagggag tgaaccaaat gggaatggag
     1321 gtctgctgca ccctcggcat gctcaacgaa gaccaggcgc gacgtctcgc cgacgcgggt
     1381 ctccacgcct ataaccataa cctcgatact tcctcagaat attacaccga gatcatttcc
     1441 acccgcacgt atgacgaccg gctgaccaca ctggacaatg tccggaaagc aggtgttaca
     1501 gtatgctgcg gcggcatcat cggactcggc gaaacgcatg cggacaggat cggaatgctc
     1561 catacactcg ccaccctgcc gcaacacccc gaaagcgtac cggtcaatgc cctcgtacgc
     1621 gtcaaaggga ctccactgga gaacaacccg aaagtagatg tatgggatat gatccggatg
     1681 atcgcaaccg cgcgcattat catgccatcc accatggtcc gtctcagcgc gggcaggtcg
     1741 gagatgagcc aggccgaaca ggccctttgc tttatggcgg gagctaattc catctttgcc
     1801 ggtgagaagc tgctgaccac acccaatccc tctttcgagg aagataatgc gatgttccag
     1861 ctcctggggc tgaaaccgag aatggcattt aaagaggaaa atcagccggc ctgaaaggcc
     1921 gaacgctaaa aacgcgccac cgggcgcatc tggcccacct cttcacagat ataggtcttg
     1981 ccggccaata cttcgtcgat ggcggtgacc atttcttcac tcggagaatt cttggttaca
     2041 tagccgttgg ctccgctgcg caacatttcc gtgacatagg accgttcggc ctggacggaa
     2101 accccaatga tcttgatatc cggcgcaaac ccgcggatcg ttcgggtcgc ttccagcccg
     2161 ttgacgggat tcatgtgaat atccatcagc atgacatcag gttgcagctg tctggcggcc
     2221 tcgatggctt ccagaccatt ggcgcactgc gctatgacat ttagacccgg cacctggctg
     2281 agtacccaac tccaggcttc acggacatgc atatgatcgt ctacgatgac taatctgatc
     2341 atggaaggtt ttttctgcca gtctcaaaat taatgtttag atcataataa aaaagtaaat
     2401 ccggggaaac catatgcgat tacccatttt ccattaagca ataaacctta tgaaaagata
     2461 aagggccggg cgctcagtgt gctcggccct tcccttaaaa caaccaacat gaaaatcttt
     2521 atatcaacag ctttcaggtc aggaaagttc actgcaccag gctcaattcc agcacctgtt
     2581 gcatcgtttt tacaaaattg aatttcagcc cccggatgaa agaggaatct atctcctgca
     2641 catccttttc attctgccag caaaggacca cctcctttag tccggccctt ctcgcagcca
     2701 gtatcttttc cttgatgcct cccaccggca aaacctggcc cctcaaggtt atttcacccg
     2761 tcatggccag atagggcttt actcgtcgtc ccgtaaaagc ggaggcgatc gagctcatca
     2821 tggttacccc cgcgctgggc ccgtccttgg gaacagcacc ctccggaaca tgcacgtgaa
     2881 tggttttttt attgaatata tccggatcga tatcgtattt ccgggcattc gactgcaaat
     2941 aggtcagcgc agtggcggcg ctctccttca taacatttcc cagattgccg gtcagcttga
     3001 gatcgccctt gccgtcactc agggaggtct ccacgaaaag aatgtcgccg cccacatagg
     3061 tccacgccag tccgacggct accccggcca tattggctac cttataaaga tcgttgctgt
     3121 acctcgccct ccccagtatc ttatcgatgt cggtctgcgt cagctgtgcc tttagcttcc
     3181 ctttcatcgc gtactccttg gccagccacc gcataatcga cgctagctga cgatccagtt
     3241 cgcgaacacc gctttcc
//
LOCUS       DASYSV010000427         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_469934, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000427 DASYSV010000000
VERSION     DASYSV010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469934"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..721)
                     /locus_tag="VGQ51_13065"
     CDS             complement(41..721)
                     /locus_tag="VGQ51_13065"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HEV9037555.1"
                     /translation="MVRIVLALLIGISLCSACQAQNDTIPAPVKGKHRHKFNIYRVDS
                     LTKAVHNPHKATLYSTFFPGLGQIYNRKYWKVPIVWAAVGIPVYTYFNNKSWYQKCQF
                     AISLYDTYAALGLPIPQDSLDMVDPKLQGFMNAEQEPSLRTYRNEFRKNQDYSVLFFL
                     LFWGLNIVDATVDAHLMNFDISNQLTMHLQPPSTPATLPGPMGTGMGLSLVFDFHKPR
                     KNYRTFAP"
     gene            complement(726..1646)
                     /locus_tag="VGQ51_13070"
     CDS             complement(726..1646)
                     /locus_tag="VGQ51_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV9037556.1"
                     /translation="MTTPHKKDKEALGKGIRSLLQSIDADLKTTSGELKSSVVEAVTN
                     MLRIQLDQIETNPRQPRHDFDETSLQELAHSIRLHDIIQPVTVSKLPSGKYRLISGER
                     RYRAAKMAGLNDIPAYVRQADDQQLLELALLENLQREDLNAMEIALSYKRMMEELNYT
                     QEQVAERMGKERSTVANYIRLLKLPPDIQVAVRNNQLSMGHARALVNVDTVDKQIYLF
                     GEIKEKGLSVRQTEELVRKLYKENSPVKNSVKTGLPEAFKRIEDNLASHFSTRVRLNH
                     NKKGEGSISIEYYSLQELNKLLDQLGVSIN"
     gene            complement(1709..2509)
                     /locus_tag="VGQ51_13075"
     CDS             complement(1709..2509)
                     /locus_tag="VGQ51_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV9037557.1"
                     /translation="MAKTIGVANQKGGVGKTTSAINLAASFAVLEFKTLLVDADPQAN
                     STTGVGFDLHNVTQSLYDCMVNHIPVKDVILKSEIPHLDVVPSHIDLVGYEIEMINYP
                     NRESVLKGILEPIKDEYDFIIIDCSPSLGLITVNALTASNSVIVPVQTEFFALEGLGK
                     LLNTVKIVQSRLNPQLAIEGILMTMYDGRLRLCNQVVSEVRKHFDEIVFDTIIHRNTK
                     ISEAPSVGKPVILYDALSKGSVNYLNLAKEILQKNNMTRIRQEERILE"
     gene            2614..3843
                     /locus_tag="VGQ51_13080"
     CDS             2614..3843
                     /locus_tag="VGQ51_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV9037558.1"
                     /translation="MRSPRFILIFILIALVLDFYVFQALKAITQGMAPRWRTAIFTVH
                     WAVSAVALITVFVTTRMHLGTRISSYLIAIVLGLYIAKVIAAVFFLIDDIRRLIQWTV
                     TRVGHHTESTDGDTISRSAFLSWIGVGLGTTVYSTFLYGFSNIYNYKIHRIKLAFDNL
                     PASFKGLKIIQISDIHSGSLANRQAVSRGVDMILAEKPDLILFTGDLVNNEAGEMERY
                     QSVFSRLQAPMGVYSTLGNHDYGDYMWWETLELKKANLDRVKQIEREMGWRLLTNEHV
                     ALERGGEQIALIGVENWSAKARFPKYGRMDLAYAGAEKYPFKILMSHDPSHWDAEIRP
                     NYPDIDLMLSGHTHGMQYGVEIPGIKWSPVQYLYKEWAGLYETGRQKLYVNRGYGFIG
                     YPGRFGILPEITVIELA"
     gene            3947..4582
                     /locus_tag="VGQ51_13085"
     CDS             3947..4582
                     /locus_tag="VGQ51_13085"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEV9037559.1"
                     /translation="MNKLALKKSTGKLAFCLLLLTATVIRSQAQSSLELGLKAGGDLM
                     KIGGRSFDQKHYPGFVAGVFGKLNFTSRWSLQPELIWNQTVGKTSADFNQIYLGISDQ
                     QVILNYVALPVMVAYKPVPELSILFGPQYGFLVQQTQNLLPHEPGVNAFNKSDLSLVF
                     GAQLNLGKVIFGVRYSAGLNNICFRDTDPWRQYGLNAYIGYQFWDIKLKKK"
     gene            4685..5275
                     /locus_tag="VGQ51_13090"
     CDS             4685..5275
                     /locus_tag="VGQ51_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEV9037560.1"
                     /translation="MKRSAFVLFIPLLFAITTQAQGFHLGVKAGANLFKVDGESFSQE
                     FQFGYAVGAFSEVYFTPNLGIQPEVMFNQTNYRTGTSFNNVYDVSGYNDIKGKLNYLS
                     IPLLLSFRPIPFLSLLVGPQFGILLNPDEHLVDNAKQAFKSGDLSLIGGAQLNLAALK
                     LGARYVVGLNNINNIQSSDVSWKNQGWQVYAGFRIF"
     gene            5398..>6029
                     /locus_tag="VGQ51_13095"
     CDS             5398..>6029
                     /locus_tag="VGQ51_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV9037561.1"
                     /translation="MKEHLFLRAEDDLDFMPIIPLNETDNEGLNDIEIPKELPVLPLR
                     NTVLFPGVVLPITVGRDKSIKAVNDAYKTDRLIGVLAQKDSSIEDPTVTDLEDVGTVA
                     RIIKLIKMPDGGTTVILQGRRRFKVGAVTAEDPYFKAQVVLLAEDEAPKEQDFEAYVS
                     NIKDLAAQIIQLSPNIPSEASIILKNIENPSFLIHFISSNLNTELHEKQQL"
BASE COUNT         1404 a         1467 c         1648 g         1510 t
ORIGIN      
        1 atggccttac ccatttttcc gtatccgatg agcgcaatct tcatggcgca aacgtacgat
       61 aatttttcct gggcttgtgg aaatcgaaga caaggctgag acccataccc gtacccatcg
      121 ggccgggtaa tgttgcggga gtgctgggcg gctgaagatg catggtcagc tggttgctga
      181 tatcgaaatt catgaggtgc gcatcgactg tagcatccac gatattaagt ccccagaaca
      241 gcaggaagaa gaggacggaa tagtcctggt tcttccggaa ttcgttgcga taggtccgga
      301 gcgagggttc ctgttccgcg ttcataaagc cctgcagttt ggggtccacc atatcgagcg
      361 agtcttgcgg gatgggaagc cccagtgcgg catatgtgtc gtacaacgag atggcgaact
      421 ggcatttttg ataccagctc ttgttattga aatacgtata cacggggatg cccactgctg
      481 cccacacgat agggaccttc cagtatttcc ggttgtagat ctgacccaga ccgggaaaaa
      541 aagtggagta gagggtagcc ttatgggggt tatggacagc cttggtcagc gagtcgacgc
      601 ggtagatatt gaatttgtgg cggtgcttgc cttttacagg agcgggaatg gtatcattct
      661 gcgcctggca ggcggaacac agggaaatac cgatcaaaag ggcgagtaca atgcgaacca
      721 tcttcttaat tgatagagac acccagctgg tccaacaatt tattcaattc ctggagcgag
      781 tagtattcga tggaaatgct gccctctcct tttttattgt ggttgagccg tacccgggtg
      841 ctgaaatgtg aagctaagtt atcttcaatt cgtttgaacg cttcgggcaa cccggtcttt
      901 accgaatttt taacagggga attctcttta tacagcttgc gaaccagttc ttcggtctga
      961 cgaacggaaa gacctttttc cttgatctcg ccgaacaggt agatctgttt gtccaccgta
     1021 tcgacattga ccagcgcgcg cgcatgaccc atcgacagct ggttattccg gacggcgacc
     1081 tggatatcgg gcgggagttt taaaaggcga atataattgg ccaccgtgct cctttctttt
     1141 cccatccttt ccgccacctg ttcctgtgtg tagttgagtt cttccatcat ccgcttgtaa
     1201 ctgagcgcga tctccatcgc gttgaggtct tcgcgttgca ggttctcgag cagcgccaac
     1261 tccagcagct gctgatcgtc cgcctgccgg acataggcgg gtatatcgtt caggccggcc
     1321 attttcgcgg cgcggtagcg acgttcaccg gatatcagcc gatatttgcc cgacggcagc
     1381 ttagagaccg tcaccggttg gatgatatcg tgcagccgga tcgaatgggc cagttcctgc
     1441 aaggacgtct cgtcgaaatc gtgacgtggc tggcgcggat tggtctcgat ctggtcgagc
     1501 tggatgcgca gcatgttcgt caccgcctcc acgacggagg atttcaattc gccggaagtc
     1561 gttttcagat cggcatcgat gctttgcaac agggacctga ttccttttcc cagggcctct
     1621 ttatcttttt tatggggagt agtcatttta aacttatttg gcgattcttg cgggggcgga
     1681 tatggcggtt gtccggcggt gagggtgttt attcgaggat cctctcttcc tgtctgatcc
     1741 gggtcatatt gttcttctgc aggatctcct tagccaggtt gagataattg accgagccct
     1801 tgctcagcgc gtcgtacagg atgacgggtt tgccgacgct gggggcttcg ctgatcttcg
     1861 tattccggtg gatgatcgtg tcgaaaacga tctcgtcgaa atgttttctc acttcgctga
     1921 ccacctggtt gcacaggcgc aaccgtccgt cgtacatcgt catcaggatg ccttcgatgg
     1981 ccagctgcgg attgagccgg ctttggacga tcttgacggt attcagcagc ttgccgagac
     2041 cttcgagggc gaagaactcc gtttggacgg ggacgatcac cgaattggaa gcggtaagag
     2101 cgttcacagt gatcaggcca agcgaagggg aacaatctat gataataaaa tcatattcgt
     2161 cctttatggg ctcgaggatg cccttgagca cgctttcccg gttaggatag ttgatcattt
     2221 cgatctcgta tccgaccagg tcgatatggg aggggacgac gtccaggtgc gggatctcgg
     2281 atttaaggat gacgtctttg acggggatat ggttgaccat gcagtcgtac agactctggg
     2341 tcacattatg cagatcgaaa ccgacccctg tggtgctgtt tgcctgcgga tcggcatcga
     2401 ccagcagggt tttgaattcc aatacggcaa agctggccgc caggttgatc gcagaagtgg
     2461 tcttgcccac tccacctttt tgatttgcaa ctccgatagt ttttgccata atagttaacg
     2521 cggccaggcg gcgcgaggca aatttacatt cttcggcata aattctccga actttcggga
     2581 gtttagcgca attttgcggt aattatcgac ttcatgcgta gcccgaggtt tatcctgatc
     2641 ttcattctta tagcattagt actagacttt tacgtattcc aggccttaaa agcgatcacc
     2701 caggggatgg cgccgcgctg gcggacggct atctttacgg ttcactgggc cgtgtccgcg
     2761 gttgcgctga tcaccgtatt cgtgacgacg cggatgcatc tggggacccg gatcagcagc
     2821 tatctgattg cgatcgtcct gggactgtat atcgccaagg tcatagccgc cgtttttttc
     2881 ctgatcgacg atatccggcg actgatccaa tggacagtca cccgggtggg ccatcatacc
     2941 gaaagtacgg atggggatac catcagtcgc tcggcttttc tgagctggat aggcgtaggg
     3001 ttgggcacga ccgtttattc caccttcctg tatgggttct cgaatatcta caactataag
     3061 atccacagga taaagctggc gttcgacaat ctgcctgcct cttttaaggg attgaagatc
     3121 atacagatat ccgatatcca ttcgggcagc ctggcgaacc ggcaggcagt cagcaggggt
     3181 gtggatatga tcctggccga aaaacccgat ctgatccttt ttaccggcga cctggtgaac
     3241 aatgaggccg gtgagatgga gcgttaccaa tccgtcttca gcaggctcca ggccccgatg
     3301 ggggtttatt ccaccctcgg caatcatgac tatggagatt atatgtggtg ggagacgctg
     3361 gaattaaaaa aggccaatct cgatcgcgtc aaacagatcg agcgggagat gggctggcgg
     3421 cttttgacga atgaacatgt cgctctggaa cgcgggggtg aacagatagc cctgatcggt
     3481 gtcgagaact ggagcgcgaa ggcgcggttc cccaaatacg gccggatgga cctggcttac
     3541 gccggagcgg aaaaatatcc cttcaaaata ctcatgagcc atgatcccag tcattgggat
     3601 gccgagatcc gtccgaatta tccggatatc gatctgatgt tgtcgggtca cacgcacggc
     3661 atgcagtatg gcgtcgagat cccgggcatc aaatggagcc cggtacaata tctctacaaa
     3721 gagtgggcgg ggttatatga aacggggcgt caaaaattat atgtcaaccg tggatatggt
     3781 tttatcggtt atcccggtcg tttcgggata ttgccggaga tcaccgtcat cgagctggca
     3841 tagagggaga gggaaagaat tttaacaaac caataaacta ttcaagcgta ttttcatcca
     3901 ataattggga acttttaaaa cagcgtcatc aacaatgaaa tcgagcatga acaaactggc
     3961 tttaaagaaa tcgaccggca agctcgcctt ttgcctgcta ctgctaactg ctaccgttat
     4021 ccgctcacag gcgcaatcgt cgctcgaact gggcctcaag gctggcggcg atctgatgaa
     4081 gatagggggc agatcgttcg accagaagca ctatccgggt tttgtcgcag gcgtattcgg
     4141 gaagctgaac tttacttccc ggtggtcgct gcagcccgag ctgatctgga atcaaacggt
     4201 gggaaagacg tccgcggatt tcaaccagat atatcttggc ataagcgatc agcaggtgat
     4261 cctgaactat gtggcattgc cggtgatggt agcttacaaa ccggtgcccg agctttcgat
     4321 cttattcggc ccgcaatatg gattcctggt gcagcagacg cagaatttgt tgccgcatga
     4381 gccgggcgtc aacgctttta ataaaagcga tctatcgctg gtattcggcg cacagctcaa
     4441 cctgggtaag gtgatcttcg gggtcaggta ttctgccggg ctgaacaaca tctgtttcag
     4501 ggacaccgat ccctggcggc aatatgggct caacgcgtat atcggatacc agttctggga
     4561 cattaaactc aaaaagaaat aaagacggca catgaattga atagtacttg taatcctttg
     4621 aacggccagg ggccgttcga ccgaatggtc agactttatg attccatcat taaaacaatc
     4681 catcatgaaa aggagtgcat tcgtattatt catcccattg ctgttcgcca tcacgacgca
     4741 ggcgcagggt tttcatctcg gcgtcaaggc gggggctaat ctgtttaaag tagacggcga
     4801 atccttcagc caggaatttc agttcggtta tgcggtaggg gcattttccg aagtctattt
     4861 tacccccaac ctgggtatcc agcccgaggt gatgttcaat cagaccaatt acaggaccgg
     4921 aacatcgttc aacaatgttt acgatgtcag cggatataat gatatcaagg gtaaactgaa
     4981 ctatctcagc atccccctgc tgttgagctt ccggccgatt ccctttctga gtctgctggt
     5041 gggacctcag ttcggcatcc tgctcaaccc ggatgaacac ctggtcgaca acgcgaagca
     5101 ggcattcaag agcggggatc tttcgttgat cggcggagcg cagctcaatc tggctgcgtt
     5161 aaaactggga gcgcggtatg tcgtcggact gaataacatc aacaatatcc agagcagcga
     5221 tgtgagctgg aagaatcagg gatggcaggt ctatgccgga ttccggattt tctaaatttt
     5281 ggcaaatttt ttttggcacc cctccctgtc gggaggggtt tattttgccg catacacggc
     5341 ctcttttgat aaaaaaggta ggttttttcc cttacctgtg gtaatttggc aatgaatatg
     5401 aaagagcatt tattcttacg tgcggaagat gatctggact ttatgcccat catacccttg
     5461 aacgaaactg acaatgaagg actgaacgac atcgagatac ccaaagagct gcctgtattg
     5521 cccttacgga atacggtgct tttcccgggt gtggtactgc cgataaccgt cggccgtgac
     5581 aaaagcatca aggccgtgaa cgacgcctat aaaacggaca ggctgatcgg ggtgcttgcc
     5641 caaaaagaca gtagcatcga agacccgacc gttacggatc tggaagatgt cggtacggta
     5701 gcccgcatca tcaagctcat caagatgccg gacggcggga ctaccgtgat cctgcagggc
     5761 agacggcggt tcaaagtagg cgcggtgact gccgaggatc catatttcaa agcgcaggtg
     5821 gtattgctgg cggaagacga ggcgcctaag gaacaggatt tcgaggccta tgtctccaat
     5881 atcaaagacc tggccgcgca gatcatccag ctgtctccca atatcccttc ggaggcgtcc
     5941 atcatcctga agaatatcga gaacccttcg ttcctcatcc atttcatatc gagcaatctg
     6001 aacaccgagc tgcacgagaa gcagcagct
//
LOCUS       DASYSV010000428        18022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1618973, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000428 DASYSV010000000
VERSION     DASYSV010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 18022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18022
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618973"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VGQ51_13100"
     CDS             <1..376
                     /locus_tag="VGQ51_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV9037562.1"
                     /translation="SERIQFISDQLNRFMEDARPFLRFGYGIRDLADDVKIPAYQLSA
                     YLNREIGLNFNDFLNQHRVRYCEELIQKGLVAQLNLKGLALQCGFNNRNTLTTAFKKF
                     TGFTPSRYQKGWEKLLNSDLVE"
     gene            complement(419..730)
                     /locus_tag="VGQ51_13105"
     CDS             complement(419..730)
                     /locus_tag="VGQ51_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037563.1"
                     /translation="MGTKRRQFSHDEKMRILQYAAQSGVTNVLHEYKLSYSVYARWKR
                     QLIPPDPNAPPRVPVHRSKIALESLLEENVRLKKIIAEQALELEMKEEELKRYVSHER
                     K"
     gene            complement(750..1313)
                     /locus_tag="VGQ51_13110"
     CDS             complement(750..1313)
                     /locus_tag="VGQ51_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9037564.1"
                     /translation="MCKELLTAPAEQHLVKLINTGDSAVFSQLYRQYWSSLLDFAENY
                     ISDSDTCKEIVQQLFITLYVKGSRLNIHASLRSYLYGSLRNKILNHLRNQSTYLRHIR
                     GASRANSSDAADNPVEEFIDVRELKKKINSCLDKMPAKCREVYVLHKQQQYTLKKTSE
                     ILQRPVDTVEKQLRRAMHLLRANLSQQ"
     gene            1514..1939
                     /locus_tag="VGQ51_13115"
     CDS             1514..1939
                     /locus_tag="VGQ51_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV9037565.1"
                     /translation="MKNKLLSFLKAVFGHKKIRVDGRQQDYAATDYIQHRLDLYMVEM
                     RPFLQPHYTIKQLAGAIRIPSYQLSAIINQRKGMNFTDYLNKLRIQYCEDLIRIEGGK
                     KINLGQLASRCGFRNRNTFVHAFRKFTGLTPSEYVRQMP"
     gene            2225..3430
                     /locus_tag="VGQ51_13120"
     CDS             2225..3430
                     /locus_tag="VGQ51_13120"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV9037566.1"
                     /translation="MTCSRNILLGVVLAVNMEACNNAMPSDLTNAAAGIASQKTEAAI
                     SCVGNSGARSAKGAGEGRVNAGAGPISANGAAFGPTRLNTFRPEHIPAGMVWIPGGEF
                     SMGNADPVGTPDGGHERMNDARPVHRVYIDGFLMDATEVTNNEFAAFVKATGYVTVAE
                     QKPTRTEFPDAKEENLVTGSIVFTPPPGAVPLDNLLRWWSYVERADWRHPTGPESSIT
                     GKGNYPVVQIAWVDAEAYAKWAGKRLPTEAEWEFAARGGKAGELYTWGDELTPGGRWM
                     ANTFQGRFPGLDDAEDGFAGIAPVAQYPPNAFGLYDMAGNVWEWCSDWYRSDYYSQLS
                     TKGVSVDPAGPETPYDAAAPGEMKKVQRGGSFLCTDQYCTRYMVGTRGKGEWRSAANH
                     IGFRCVRHP"
     gene            3528..4514
                     /gene="ligD"
                     /locus_tag="VGQ51_13125"
     CDS             3528..4514
                     /gene="ligD"
                     /locus_tag="VGQ51_13125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEV9037567.1"
                     /translation="MFLAPGPDMDPKSTKKGRRSPIPSRVAPMLCTLTREPADDPDYL
                     YEIKWDGYRIISYVDKGKVKLDSRSAKDYTARYPVVTEALRQLGHNVVIDGEVVVFNE
                     EGLPDFDALQLYNGHSSPITYCVFDLLWLDGYDLMQLPLSARKQLLQDLVQDRPGLRF
                     SESFDDGPRLYKEMLRINLEGIIAKKRNSPYVPDSRGMDWLKTPTRKRQEFVIGGWSE
                     SDKNRSFKSLLFGAYHKGKLEWIGRSGGGYKEKEMPDILKKLMRLETTRSPFVNPVLD
                     TKGAVTHWVKPELVANFEFATWTKSGRIRKPATFLGFRKDKKPTQVVREVPK"
     gene            complement(4516..5016)
                     /locus_tag="VGQ51_13130"
     CDS             complement(4516..5016)
                     /locus_tag="VGQ51_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEV9037568.1"
                     /translation="MPDELDAIDIRILQLLQHDSRITTKEMADKLGKSVTSVYERIRK
                     LEDSGIIQRYVALLNKDLIGKHLIAFTTVQLKEHSQAALRGFEKEGIKFQEVMECYHL
                     TGTFDFLLKIAIQDMKEYNAFLMNKLATLPNIGVVQTFFVLSAGKVETAFALPAPAGA
                     KRPPKR"
     gene            5167..6165
                     /locus_tag="VGQ51_13135"
     CDS             5167..6165
                     /locus_tag="VGQ51_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV9037569.1"
                     /translation="MKKDTLLVIGACGQIGVELIAALRREYGDQRVIAADLKPSTAAL
                     QAEGPYEQLDVLNRGALSALISRHDVTQVYLLAALLSAMGEQHPEKTWHLNTQSLLNI
                     LDLARERRLGRVFFPSSIAVFGPDAPRRDCPQPTALTPTTVYGISKMAGESWCRYYWH
                     KYGVDIRSIRYPGLISHRTQPGGGTTDYAVDIFHQALAHGNYCCYLRPDSVLPMMYMP
                     DAIRATMQLMSVPSAWLTVRSSYNLAAMSFSPMELARAIEQYIPGFSIRFEPDYRQVI
                     ADSWPQSIDDSAARRDFNWEYEYGLSATVRDMLLHLPKKTGAQGHPLPRLQPDIFF"
     gene            6206..8650
                     /gene="ppsA"
                     /locus_tag="VGQ51_13140"
     CDS             6206..8650
                     /gene="ppsA"
                     /locus_tag="VGQ51_13140"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEV9037570.1"
                     /translation="MQERVILPLKSVGLADIALVGGKCASLGEMIQHLAPLGVQIPDG
                     FVITTDAYWQFLEQSQLASFIREQLDQIDFNKVESLRRAGSRIRQAIRNVRFPHELSH
                     PIIAAYEELSHSYDQSATDVAVRSSATAEDLPDASFAGQQETFLNVRGPAALIDAVRN
                     CYASLFTDRAISYRRQFKYDVSPVGLSVCVQKMVRSDLASSGVAFSLDTETGFPDIVM
                     INGSWGLGEMVVQGAISPDEFLVFKPARKKGYDSIIEKRLGVKDKLMVYGDDSDERTR
                     VIPAEENQKHRFCLTDAQVLQLADWVMSIEDYYTAVKAKWCPVDIEWAVDGLSGHLFI
                     VQARPETVHSRKKQNVFAEYRIGDPDTPKKLIGKGIAVGDKIAAGQVNILYSLDKRIG
                     GHEFMDGEILVTDMTDPDWEPIMKKASAIITNKGGRTCHAAIVAREMGVPAIVGCGNA
                     TEWLKEGQWVTASCAEGEEGYIYEGKLEFSCVATDLDDLPIVRTPVMLNVGSPATAFG
                     YAHLPHSGVGLAREEFIINTYIRAHPLALLQHREVKDEALSTAIASLIRGFENEEAYF
                     IRKLSFGIAKIAAAFYPQKVIVRFSDFKSNEYRGLLGGHHFEPTEENPMIGWRGASRY
                     YSPAYKPAFGLECRAIRAVREEMGLENVVVMIPFCRTVEELLKVKATMREYGLEQGVA
                     GLELYLMAELPSNILLAREFARHIDGFSIGSNDLTQLTLGLDRDSGLVAHLYDERNDA
                     VKSLLRMLIRTAKECRIKVGICGQGPSDFPDFAQFLVEEGIDSISVTPDSIIKTIRAI
                     DAIERASLPISEALRS"
     gene            complement(8604..8957)
                     /locus_tag="VGQ51_13145"
     CDS             complement(8604..8957)
                     /locus_tag="VGQ51_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9037571.1"
                     /translation="MKKIILAEDDPGIQDSIQMILEREGYEVTIYANGEDLLNKRFQT
                     PDLFILDRQLSGVDGLDICKFLKKDTEVNQIPVIILSASPQIGPLAKKVGADDFLEKP
                     FTIEGLRKLVAKHVL"
     gene            complement(8970..12992)
                     /locus_tag="VGQ51_13150"
     CDS             complement(8970..12992)
                     /locus_tag="VGQ51_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEV9037572.1"
                     /translation="MYPSGPLFSTDKYEDNKGKAASHEFLFVGIGASAGGIQALKTFF
                     QNVPPNSGIGYVVILHLSPDHDSKLAEVLQVATSMPVIQIVGNTAVRPDHVYVVPPNQ
                     HLEMLDGQITVSPNLQPEDRRAPVDIFFRTLAESHGNQAVCVVLSGTGANGSMGLKRI
                     KELGGSAFVQNPREAEFNEMPRNAIATDLVDEVLPVSEIPAKIISYKVSLGRGLIPVD
                     AEKRPEDLQQALREIFTQLRVRTGHDFSNYKRPTLLRRIERRVNIRNLPDLTAYAAFI
                     LQNPEEIVALLKDLLISVTNFFRDRKAFDVIDKDVLPSLLHGKTSDDQLRIWVAGCAT
                     GEEAYSLAILCAERTLSVIDAPKIQIFATDIDEAAINHARDAYYTLNDAADLSPERLH
                     RFFTREGAGFRIRREIREMVLFANHNFIKDPPFSHLDMVSCRNVLIYLNRAAQERVME
                     TFHFALNPGGFLFLGSSESADGATDLYSAYNREFHILQSRKKLVRTFHLPESVPSFHL
                     EHSRLSPNGKEHENRTLERITFGDLHQRLLEEYAPPSVILNEDYDVVHLSERAGRYLQ
                     ITGGELSQNLLKLVRQDLRLELRSALNQATQRRTAVETRALKVTIDDRTETVNIHVRP
                     VLRGGDVAQGFFLILFEPTSEEGDNKNVVLASDEPMALRLEEELVRLKARLRASIEQH
                     EFNAEELKASNEELQAMNEELRSAAEELETSKEELQSINEELRTVNQELKVKIEETSL
                     ASNNLQNLINSTSIGTIFLDRSFRIALFTPAILEIFNLIPGDYGRPLSDITHRLQYDH
                     LLHDAELVLEKLSPVEREVNTTDGHAFMMRVLPYRTAEDHINGVVITFIDMTTRKLAE
                     EKLRYSEEKYRTVLATIEDGFCIVDVMFDGQGRVVDYLFLEANASFLRHAGMPAEVAG
                     KTGREAFNDTEPRWLELFGRAASTGEFIHVEDQIGPADSHGRYEISAMRSGEPDKHRV
                     AVFFRDITERMRVEKAMRLSEERLRVALTSAEMAAWDWDVPNETIIWNDQQYILLGLS
                     EDNRTSNMASFLQSVYPDDIGRVQRQLDAAVQETGVFHCEFRIRRGEGDEIRWMRGYG
                     RAVDWQDDKATRLVGVMYDITKQKSSEQKDEFISIASHELKTPVTSIKSYVELLQEQL
                     TTKDEKDAALLKKLNGQVDRLAGLVTALLDTSRIAEGQLHLYPEEFDLNRLIGDLVEE
                     LQRTTARHKLVFKAATLEPITADKERIRQVVVNLLSNAIKYSPGGGDITITSENSSDS
                     AKVSISDHGIGVPKEHLRQVFDRFFRVTNPHIYTYPGMGLGLYISAGIIARHGGTIFA
                     DSNPGEGAVFSFTLPYKKHQ"
     gene            complement(13158..14336)
                     /locus_tag="VGQ51_13155"
     CDS             complement(13158..14336)
                     /locus_tag="VGQ51_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037573.1"
                     /translation="MKHIYAFILSMFALCGLSRAQFIAENRVTAAGAETENSNEPQVC
                     PLGPAPAAPWKKEADQVFGHIGRSLLAKLKGSSEGIISVFHDSILTQGTFDPIWHGEY
                     FSAGNGAPQMRFGVSCIFHNDDVNPNDPCDLTIFANDISPLTGSLTVNGQAFVTLKGF
                     TGSSPAFEFDMPAHGGNSAADTETVHVKAWLVAADSSMLPYIPVTRKEYLSLAQQELE
                     NRKEMLIADIKSRINIRSATQQETGKQQALDQLRKTYSGADLQTRTRIFLSSYKTDEE
                     YMKQHITAATEELDRTLALIGKLLTGSTPQQLAQPAVVSVAAVEFRGFEDGRPGSTVL
                     VRLRGDYYNADADQLTIKSLLICWRYHPADRMAAGIDQQLALRSPVPRLRKQFAAASK
                     "
     gene            complement(14509..15693)
                     /locus_tag="VGQ51_13160"
     CDS             complement(14509..15693)
                     /locus_tag="VGQ51_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037574.1"
                     /translation="MKRLYAIALLVFAMCDHSRAQFIADNHNVPTAADGPAGITEALV
                     CPMVPAISEPWQKEEDHVFGHIRGNRLAMMKNTSEDIISLLHDSILTDGSFRPIWHGE
                     YFSSGNGAPQMRFGLSCVFYNGDENSNSPGDLTIFANDISPLLGRLTVNGHEFTTLKG
                     FTGGQDRPGFEFDMPADAGDSVVHIKAWLVTADSSVLPYIPVSRKEYLEQARQELESR
                     RDAVIADIKSRIQIRPSADQEAAKQQALDQLRATYSGIDLQAHTRVFLSNYKSDEEYM
                     KQNIAVNTEDLDRTLAIIARLITSSTTRQLAQPAVVSVAAADFHGFEDGMPNSTVLVR
                     LRPSWYGADTDQTTIKSLLICWRYRPADRMAAGIDRQLALRNPIPRLRKQFHTVSMEK
                     SE"
     gene            16088..16840
                     /locus_tag="VGQ51_13165"
     CDS             16088..16840
                     /locus_tag="VGQ51_13165"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEV9037575.1"
                     /translation="MKVSRTARGKYLPLLFLLLIATLSFDFTGKTPIPSPTSSISSIS
                     PISSVSARASGFFALYDDLRLGALDLSRDAFQHAVEGFLALRTAGRLGNQNVLSIIDF
                     SLPSSQRRLFILDMANGRLLFNTFVAHGRNSGELMATRFSNRLSSYMSSLGFYLTGRS
                     FMGKHGYSLRLEGMEKGWNDHALQRAIVMHSAPYVSEDHIRRWGSLGRSEGCPAIPAK
                     VRTAIIDEIKGGSCLFLYAPDDRYLDRADPFS"
     gene            16882..17331
                     /locus_tag="VGQ51_13170"
     CDS             16882..17331
                     /locus_tag="VGQ51_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037576.1"
                     /translation="MKTPGAPVTLGRCLLAGLLTGIVAAVIVSIFNIIYRRETELIAY
                     AVVMHLSIFMAFPFFHLVSGGFYYLFIDHLKKGTLIFVVIMLLGTVISALITAYAGNR
                     SDPTVEAFRGLLIGLEIIGGLLGAFLIPYLVRHPTLFLTAEDIKGEE"
     gene            complement(17364..>18022)
                     /locus_tag="VGQ51_13175"
     CDS             complement(17364..>18022)
                     /locus_tag="VGQ51_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV9037577.1"
                     /translation="VFLLGDAAHVHSPVGGQGMNTGIGDAVNLAWKLEAVLRDGAPDA
                     LLDTYETERIRFARRLVASTDKAFAFVNRRGSLASEVRTHIVPNLFPVLFRKRAVRRV
                     AFRVVSQTGIQYRGSALSEGTAHGIHGGDRLPWLMRQDNYGVLQSMDWQIHCYGEASK
                     DLTEWCSRSSIPLHVFSPAGAIHRGTICLIRPDGHIGWIDNRAATDELTRYVHQWRIA
                     "
BASE COUNT         3968 a         4749 c         5074 g         4231 t
ORIGIN      
        1 ctccgagagg atccaattca tttccgacca actgaaccga ttcatggaag acgcaaggcc
       61 attcctgcgt tttggatacg ggatccgcga tctggcggat gacgtcaaga tacccgctta
      121 ccagctgtcg gcgtatctta accgggagat cggtcttaac ttcaacgatt tcctgaacca
      181 gcacagggtc aggtattgcg aggagcttat tcaaaagggc ctggtcgctc aactaaacct
      241 aaaaggcctg gcgctgcagt gtggattcaa caaccgaaac acgctcacca ccgcttttaa
      301 gaaattcacg ggatttactc cgtcccggta ccagaaaggc tgggaaaagc tgctgaactc
      361 cgacctggtc gagtaaatga tctcaaaccg agagatgtgg cgaccgccac atctcttttt
      421 attttctctc gtgagagacg tatctcttca gctcttcctc cttcatctcc agctccagcg
      481 cctgttccgc aattatcttt ttcagccgca cattctcttc cagcagcgac tccagcgcga
      541 tcttcgacct atgcaccggg accctgggcg gggcgttggg atcgggaggg atcaattgtc
      601 ttttccaccg ggcatagacg ctataggata gcttatattc atgcaacaca ttggtgacgc
      661 ccgattgcgc cgcatattgc agaatcctca ttttttcatc gtggctgaat tgccttcttt
      721 tggtacccat ttcgatcaag ataaaatatt cattgttggg aaagattggc gcgcaggaga
      781 tgcatcgctc tcctgagctg cttttccacc gtatccaccg gtctttgcag gatctcggat
      841 gtctttttca gcgtatactg ctgctgtttg tgcaacacat atacctcgcg acatttggcg
      901 ggcattttgt cgagacagct gttgatcttc tttttcagct cgcgcacgtc gatgaactcc
      961 tccaccgggt tgtccgcggc gtcggatgaa ttggcgcgcg aggcgccgcg aatgtgccgc
     1021 aggtaggtcg actggttgcg caaatggttc agtatcttgt tgcgcaggct accgtacagg
     1081 taagaccgaa gcgacgcatg gatattgagc cgcgatcctt ttacatataa tgtaatgaac
     1141 agctgttgaa cgatctcttt acacgtatcg ctgtccgaga tatagttctc ggcgaaatcc
     1201 agcaacgagg accagtactg ccgatacagc tgggagaaga cggcgctatc gcctgtgttg
     1261 atcaatttta cgaggtgttg ttctgcggga gcggttagta attccttaca cataaagggt
     1321 cgatttttct ctgataaaaa gcgtgattca agatagctat taatcgcgtg gatgaccctc
     1381 atcgggagag ccctatcgga ctcgttttgc aaatctgagc agaaaagatt cctatcactt
     1441 tcctgctgag atcatcggaa ggtgtctatc cgtaaacgcg cgtcgcgctc gcgttctgct
     1501 aaccggatcg gatatgaaaa acaaattgct ttcattttta aaagctgtgt tcggtcacaa
     1561 gaagatccgg gtcgacggcc ggcagcagga ttatgcggcc accgattata tccagcacag
     1621 gctcgatctg tatatggtgg agatgaggcc atttctacag ccacattata cgatcaaaca
     1681 attggcgggc gccatccgta taccttccta tcagctgtcg gccatcatca atcagcgaaa
     1741 ggggatgaat ttcaccgact atctcaacaa gctgaggatc caatattgcg aagacctgat
     1801 ccgtatcgaa ggaggaaaaa agatcaatct cggtcagctg gcgtcgcgat gcgggttccg
     1861 caaccgtaac acgttcgtac atgcattcag gaaatttaca gggctcacgc cgtcggaata
     1921 tgtccggcaa atgccgtgaa gaacgatagt gccaaagctt ataaattttg gcatagatga
     1981 gcccaaaata tttcgcaccc cttgaaaccg tccgtatctt taactcagga gttctcttca
     2041 tacttagcag gtagtcgata gggggttttt taaagacaga tggacttttg ttcatctgtc
     2101 ttctttaaaa gataactggt caatcaatat atttttatcc cgtttaattt cgaatggttc
     2161 caaaaacaca acccacagtt cagtaagcag cgtatttcta tacgcggctt ttcaaaacca
     2221 aaagatgacc tgcagccgga acatattatt aggagtcgtt ctcgccgtca acatggaggc
     2281 atgcaacaac gcgatgccgt cggatctgac caatgcagct gcgggtatcg cttcacaaaa
     2341 gacggaagct gccatttcct gcgtcggaaa cagcggggcc aggtctgcca agggcgccgg
     2401 agaaggccgc gtcaacgccg gagccgggcc tatctctgcc aatggcgccg cattcggacc
     2461 cacccgtctc aatacctttc gcccggagca tattccggcc ggcatggtct ggatccccgg
     2521 cggcgaattc agcatgggga atgcggaccc cgtgggtaca cccgacggcg gacatgaaag
     2581 gatgaacgat gcccgtcctg tccaccgggt atatatagac gggttcctga tggatgccac
     2641 cgaagtgacc aataatgaat ttgccgcctt tgtaaaggcg accggttatg tcacggtggc
     2701 cgaacagaaa ccgacgcgca cggaatttcc ggatgcaaaa gaagaaaacc tggtcaccgg
     2761 ctctatcgtg ttcacccctc cgcccggtgc agtaccgctg gacaatttgc tgcggtggtg
     2821 gagctatgtc gaaagggccg actggagaca tccgacggga ccggaaagct ccatcacggg
     2881 aaaagggaat tacccggtag tgcagatcgc ctgggtagat gcggaggcct atgcgaaatg
     2941 ggcaggcaag cgattgccga cggaagcgga atgggaattc gcggcccgcg gaggaaaggc
     3001 aggcgaactg tacacctggg gagacgagct cacaccgggc ggccgctgga tggccaatac
     3061 cttccagggt agattccccg gactcgatga tgcggaggac ggtttcgccg ggatcgcccc
     3121 ggttgcccaa tacccaccca atgctttcgg tctctacgat atggccggca atgtctggga
     3181 atggtgcagc gactggtacc ggtccgatta ttacagtcag ctttcaacaa agggagtatc
     3241 cgtagatccc gccggacccg agactccgta tgacgcggcg gcgcccggag aaatgaaaaa
     3301 ggtgcagcgg ggcggttcgt tcctgtgtac ggatcagtac tgcacacggt atatggtggg
     3361 cacgaggggc aagggagaat ggcggtctgc ggccaatcac attggcttcc gctgcgtgcg
     3421 acacccctga caacgagtgg atggttcttt ttcatagcag ggtttttaag ggcaaagggt
     3481 cccgcttcag cgggaccctt tttttgtgcc tatgcattcg ggctggtatg tttcttgcgc
     3541 cgggacctga tatggacccg aaaagcacaa aaaaagggcg aagatcgccg atcccttcga
     3601 gggtggcccc gatgctttgc accctgaccc gggagcccgc cgacgatccc gactatctct
     3661 acgaaataaa atgggacgga tatcgcatca tctcctatgt cgataaaggc aaggtcaagc
     3721 tggattcgcg cagcgcaaag gactatacgg cgcggtatcc ggtggtcacc gaagccctcc
     3781 ggcaactggg tcataatgtg gtcatcgacg gggaggtcgt cgtcttcaac gaagaggggc
     3841 tgccggattt cgatgccctg caactgtata acggtcattc ctcccctatc acctattgtg
     3901 tgttcgatct gttgtggctg gacggttatg acctcatgca gctgccgctg tcggcgcgta
     3961 aacagctgtt acaggacctg gtgcaggatc ggccggggtt acgattcagc gagtcgttcg
     4021 atgacggtcc gcggttgtat aaggaaatgc tgcgcataaa cctggaaggg atcatcgcaa
     4081 aaaaaagaaa tagcccttat gtgcccgact cgagggggat ggactggttg aaaacgccga
     4141 cgcgcaagcg acaggaattc gtcatcggcg gatggtcgga gtcggataag aaccggtcgt
     4201 tcaaaagcct gcttttcggc gcttatcaca agggtaaatt ggaatggatc ggccgctcgg
     4261 gcggcggcta taaagaaaaa gaaatgccgg atatactgaa aaaactaatg cggctcgaaa
     4321 ccacccgctc ccctttcgtg aatcccgtac tggatacaaa gggggcggtg actcattggg
     4381 tcaaacccga actggttgcc aatttcgaat tcgccacctg gacaaagtcg ggaaggatca
     4441 ggaagcctgc cacttttctt ggcttccgga aggacaagaa acccacccag gtagtgcggg
     4501 aggtgccgaa ataaattacc tttttggcgg gcgcttcgcg cccgccgggg ccggcagggc
     4561 gaaggccgtc tcgacctttc ccgcggaaag cacgaagaat gtctgcacga cgccgatatt
     4621 gggcagcgta gccagcttgt tcatcaggaa ggcgttgtac tccttcatat cctgaatggc
     4681 tattttcagc aggaaatcga aggtcccggt gagatgataa cactccatca cttcctggaa
     4741 tttgataccc tctttttcga accctcgtaa tgccgcctgc gaatgctctt tcagctgcac
     4801 ggtcgtgaag gcgatgagat gtttcccgat aaggtctttg ttgagcaatg ccacataccg
     4861 ctgtatgatc ccgctgtcct ccagtttcct gatccgttcg tatacggaag tcacggactt
     4921 gccaagtttg tcggccatct cttttgtggt gatgcgcgaa tcatgctgca acaattgaag
     4981 gatacggata tcgatggcgt ccaattcgtc cggcataagc gattttttta tggaaagaaa
     5041 aaatgtaatc ggttcaaaac cgaatgaatt tccgaaaaga tgaccgaaaa tagattttaa
     5101 ttctatgaaa ttgaaataaa ctggaattct taacgagaat ttgaacattt gtctaaatcg
     5161 ttgccgatga aaaaggatac gctgctggtc attggagcct gcggacaaat aggcgtagag
     5221 ttgatcgccg ccctgagaag agaatatggg gaccagcggg tgatcgctgc cgatctgaaa
     5281 ccgtctactg cggcattaca ggccgagggc ccttacgaac agctggatgt gctgaaccgg
     5341 ggcgcccttt cggccctgat ctcccggcac gacgtcaccc aggtctacct gcttgccgcc
     5401 ctgctctcgg ccatgggtga acaacatccc gagaagacct ggcacctgaa tactcaaagt
     5461 ctgctcaaca tcctcgatct ggcgcgtgaa cgaaggctgg gaagagtatt ctttcccagc
     5521 agcatagccg ttttcggtcc cgatgccccc cgtcgcgact gtccgcagcc aacggcgctg
     5581 acgccaacca ctgtttatgg tatcagcaag atggcgggcg agtcctggtg cagatattat
     5641 tggcataaat atggtgttga tatacgcagt attcgttatc ccggtctcat cagccatcgg
     5701 acacagcccg gcggcggcac caccgactac gcggtcgaca tattccatca ggcgctggcc
     5761 catggaaatt actgttgcta tctgcggccc gatagcgttc tgccgatgat gtatatgccg
     5821 gacgccatcc gcgcgacgat gcagctgatg tcggtgccct cggcctggct taccgtaagg
     5881 tcctcgtaca acctggcggc catgagcttt tcgccgatgg agctggcaag ggccatagaa
     5941 caatatatcc ccggattcag tataaggttc gagccggatt accgccaggt catcgcagac
     6001 agctggccgc agagcatcga tgattccgcc gcccgcaggg atttcaactg ggaatatgaa
     6061 tacggtcttt ccgcaaccgt gcgcgacatg ctgctgcacc tgccgaaaaa gaccggcgcc
     6121 cagggccatc ctctgccacg gctgcagccc gatattttct tttaacaagg gcctgtgtaa
     6181 accagaccca taaagatcct agtgtatgca agaacgagtt atccttcccc taaaaagcgt
     6241 aggcctggca gatatcgccc tggtcggcgg caaatgcgcc tctctcggcg agatgatcca
     6301 gcacctcgca cctctcggcg tccagatccc cgacggattt gtcattacca ccgacgccta
     6361 ctggcaattc ctcgaacaaa gtcagctcgc gtcctttata cgcgaacagc tggatcagat
     6421 agatttcaat aaggtggagt cgctgcgtcg cgctggatcg aggataagac aggcgatccg
     6481 gaatgtccgg ttcccgcacg agctgtcaca tcccatcatt gccgcctatg aagagctgtc
     6541 gcattcctat gaccagtccg cgacagacgt agcggtccgg tcctccgcca cggcggaaga
     6601 tctgccggat gcctcctttg cgggccagca ggaaaccttt ctcaacgtcc ggggtcccgc
     6661 cgcgctgatc gacgcggtca ggaattgcta tgcttcgctg ttcaccgaca gggctatcag
     6721 ctatcggcgg cagttcaagt acgatgtatc cccggtgggt ctttcggtgt gcgtacaaaa
     6781 aatggtgcgc tccgatctgg caagtagtgg cgtggcattt tcgctggata ccgagacggg
     6841 tttccccgac atcgtgatga tcaacgggag ctgggggctc ggggaaatgg tggtgcaggg
     6901 cgcgatctcg ccggatgaat tcctcgtatt caagccagcc cgcaaaaagg gctacgattc
     6961 catcatcgaa aagcggctgg gcgtaaagga caagctgatg gtctatggcg acgacagcga
     7021 cgagcgcacc cgtgtcattc ccgccgaaga aaatcagaaa caccgttttt gcctcaccga
     7081 cgcccaggtc ctccagctgg ccgattgggt catgagtatc gaagactatt atacggccgt
     7141 caaggcaaaa tggtgcccgg tagacatcga atgggctgtc gacggtctgt cgggtcatct
     7201 ttttatcgta caggcccggc ccgagaccgt ccattcgaga aagaagcaaa acgttttcgc
     7261 cgaataccgg atcggggacc ccgatactcc caaaaagctg atcgggaagg gaatcgcggt
     7321 cggcgacaag atcgccgcgg gccaggtcaa tatcctttat tcgttggaca aacgcatcgg
     7381 aggccatgag ttcatggatg gagagatcct ggtgacggac atgaccgacc cggattggga
     7441 accgatcatg aagaaggcct ccgcgatcat taccaataaa ggtggccgca cctgtcatgc
     7501 cgccatcgtc gcacgggaga tgggtgtacc ggccatcgtc ggctgcggta atgctaccga
     7561 atggctgaag gaggggcaat gggtcaccgc ctcctgcgcc gaaggcgagg aaggatacat
     7621 ctacgaaggg aagctggagt tcagctgcgt cgccaccgat ctcgacgatc taccgatcgt
     7681 ccggaccccg gtgatgctga acgtaggctc gccagctacc gctttcggat acgcgcattt
     7741 accgcatagc ggggtgggtc tcgcacgcga ggagttcatc atcaatacgt atatccgcgc
     7801 ccatccgctg gcgctgctgc aacaccggga ggtgaaagac gaggcgcttt ccacggccat
     7861 cgcttcgctg atccggggct tcgagaatga agaagcctat tttatccgga aactgtcgtt
     7921 cggcatcgcg aagatcgccg ccgccttcta tccgcagaag gtgatcgttc ggttctcgga
     7981 tttcaaaagt aacgaatacc gtggtctgct gggtggacat cacttcgagc ctactgagga
     8041 aaatccgatg ataggctgga gaggcgcttc gcggtattat tctcccgcct acaaacctgc
     8101 ctttggtctc gagtgcaggg cgatacgcgc ggtgcgggaa gaaatggggc tggagaacgt
     8161 ggtcgtaatg atccctttct gccgtacggt tgaagagctg ttgaaggtga aggcgacgat
     8221 gcgggaatat gggttggaac aaggcgttgc gggattggag ctttatttaa tggccgaatt
     8281 gccttccaat atcctgctgg cccgcgaatt cgcgcggcac atcgatggat tctccatcgg
     8341 atcgaacgat cttacacaat tgacgcttgg tctcgacagg gattcgggtc tggtcgcgca
     8401 tctgtatgac gaaaggaatg atgcggtgaa gagcctgctg cgtatgctga tccggactgc
     8461 gaaggagtgc cggataaaag tcggcatctg cggtcaggga ccctccgatt ttcccgattt
     8521 cgcgcaattc ctcgtcgagg aaggcatcga cagcatttcg gtgacaccgg attcgatcat
     8581 caagacgatc agggcaatcg acgctataga acgtgcttcg ctaccaattt ccgaagccct
     8641 tcgatcgtga agggtttttc gaggaagtcg tctgcaccga ctttctttgc cagggggccg
     8701 atctgcgggc tggcggagag aatgatgacg ggtatttgat tcacttcggt gtcctttttc
     8761 aggaatttgc agatatcgag tccatcgacc cctgagagct gacggtcgag gataaaaaga
     8821 tcgggagtct ggaaacgttt gttgagaagg tcctcgccgt tggcgtagat cgtgacttca
     8881 tatccttctc tctcgaggat catttggatg ctgtcctgaa tccccgggtc atcttcggca
     8941 agaattattt ttttcatttg cagccctttt tattgatgct tcttgtaagg cagggtgaaa
     9001 gaaaacaccg cgccttcacc cggattactg tctgcgaata tcgtaccacc gtgcctggcg
     9061 atgatgcctg ctgagatgta cagaccgagc cccatgccgg gataggtgta gatatgggga
     9121 ttagtgacac ggaaaaaccg atcaaacacc tggcgcagat gctctttagg tacgccgata
     9181 ccatgatcgg agatactgac cttcgcgctg tcggaagagt tctccgaggt aatggtgata
     9241 tctccgccgc caggagaata tttgatggcg ttggacagga ggttgacgac cacctgccgg
     9301 atccgctctt tatcggcggt gatcggttca agcgttgcgg ccttaaagac cagtttgtgc
     9361 cgggcggtgg tccgttgcag ctcttctacc aggtcgccga tcagccggtt cagatcgaac
     9421 tcttcgggat aaagatgcaa ttggccttcc gcgatcctcg aagtatcgag cagggcggtg
     9481 acgagaccgg ccagcctgtc cacctggccg ttcagttttt tgagcaatgc agcatccttt
     9541 tcgtccttag tggttagctg ttcctgcagg agctcgacat aagatttgat gctcgtaacg
     9601 ggtgttttca gctcatgact ggcgatgctg atgaattcgt ctttctgctc ggacgatttt
     9661 tgtttcgtaa tatcatacat gacgcctacc agccgggtgg ccttgtcgtc ctgccagtcc
     9721 actgcccggc catagccgcg catccatcgt atctcgtcgc cctcgccgcg ccggatacgg
     9781 aattcgcaat ggaatacgcc tgtttcttgc accgcagcat ccagctgccg ctgtacgcgc
     9841 ccgatatcgt cggggtagac ggattgcagg aacgacgcca tattcgacgt ccggttatct
     9901 tccgagaggc ccagcaggat gtattgctgg tcgttccaaa tgatggtttc attcgggacg
     9961 tcccagtccc atgccgccat ttcggccgat gtaagcgcaa cacgcaaccg ttcttccgac
    10021 aatcgcatgg ccttttcgac gcgcatccgt tcggtaatgt ccctgaagaa taccgctacc
    10081 cggtgcttgt ccggctcacc gctccgcatg gcgctgatct cataacggcc atgggaatcc
    10141 gccggcccta tctggtcttc gacatgaatg aattctcccg tcgaagcggc ccggccgaag
    10201 agttccagcc atcggggttc agtatcattg aatgcctcgc ggcccgtttt tcccgcaact
    10261 tccgcgggca tgccggcatg gcggaggaac gaagcgttgg cctcgagaaa aagataatcg
    10321 accacgcggc cctgcccatc gaacatgaca tctacgatac agaaaccgtc ttcgatggtg
    10381 gccaggacgg ttcggtattt ctcttccgaa taccgcagtt tttcctccgc cagcttccgg
    10441 gtcgtcatgt cgatgaaagt gatcaccact ccattgatgt gatcctccgc cgttctgtaa
    10501 ggcaacacgc gcatcatgaa tgcatggcca tcggtcgtat tcacttctct ttcgacggga
    10561 gatagttttt ccagcacgag ctcagcatca tgcagcaggt gatcgtactg gagacgatgg
    10621 gtgatatctg acaggggtcg gccgtagtcg ccaggtatca ggttgaaaat ttcgagaatg
    10681 gctggcgtga acagggcgat acgaaaactt cggtcgagga agatggtgcc gatgctggta
    10741 gaattgatca ggttctgtag gttgttgctt gccaggctgg tctcttcgat cttgaccttt
    10801 agttcctggt tgaccgtacg cagctcttca ttgatggatt gcaactcttc cttgctcgtc
    10861 tcgagctcct ctgcggcgct gcgtaattcc tcgttcatgg cctgcagctc ttcattggaa
    10921 gccttaagct cttcggcatt gaactcatgc tgttcgatgg aggcgcgcaa ccgggccttc
    10981 aatcgcacca actcctcttc cagccgcagg gccatgggtt cgtcggaggc gaggaccaca
    11041 tttttgttgt cgccctcttc ggaggtcggt tcgaacagaa tcaggaagaa tccttgcgct
    11101 acgtctcctc cgcgaagtac ggggcggaca tggatattga cggtctcggt ccgatcgtcg
    11161 atggtgactt tgagtgcgcg cgtctcgacg gccgttcgac gttgcgtcgc ctggttgagc
    11221 gcgcttcgaa gttcaagccg caggtcctgc cggaccaatt tgagaaggtt ttgcgataat
    11281 tctcctccgg tgatctgcag ataccggccg gcccgttcgg acagatgtac gacatcataa
    11341 tcttcgttaa ggatgaccga aggcggcgca tattcttcga ggagacgttg atgcaggtcg
    11401 ccgaaggtga tgcgttcgag cgtgcggttt tcgtgttcct tgccgttggg tgagagacgg
    11461 gaatgctcga gatggaagct cggcaccgat tcgggtaagt gaaaggtccg aacgagtttt
    11521 ttgcggcttt gtaagatatg gaattccctg ttataagccg aatagagatc ggtggctcca
    11581 tcggccgatt cggacgagcc gagaaaaaga aatcctccgg ggttgagcgc aaaatgaaag
    11641 gtctccatca ccctttcctg cgccgcccgg ttgagataga tgagcacatt gcgacagctg
    11701 accatgtcca ggtgtgaaaa gggaggatcc ttgatgaaat tgtggttggc gaacaggacc
    11761 atctcgcgta tctcccgccg aatcctgaat cccgcacctt ccctggtgaa gaaccggtgg
    11821 aggcgttcgg gtgacaggtc ggcggcgtcg ttaagggtgt aataggcatc gcgggcatga
    11881 ttgatggcgg cctcatcgat atcggtggcg aagatctgga tcttcggggc atcgatgacg
    11941 ctaagcgttc gctcggcgca cagtatcgcc agcgaatatg cctcctcacc cgtcgcgcag
    12001 ccggccaccc agatacgcag ttggtcatcg gaggtcttgc catgcaggag gctgggtagc
    12061 acgtctttat cgatgacatc gaaggccttg cgatcgcgga aaaaattggt gacggagatg
    12121 agcaggtcct tcagtagggc aacgatctcc tccgggttct gaaggataaa agcggcatat
    12181 gcggtaagat cagggagatt ccggatattg acgcgccgtt cgatccggcg aagcagggtc
    12241 ggccgtttgt aattggaaaa gtcatgcccg gtgcggacac gcagctgggt aaatatctcc
    12301 cgaagagcct gctgaaggtc ttccggtctt ttttcggcgt cgacgggaat aagccccctt
    12361 cccaggctca ccttgtagga tatgatcttc gcggggatct ccgacacggg cagcacttcg
    12421 tctacgagat ctgtggcaat ggcgtttcgc ggcatttcat tgaattcggc ctcgcgcgga
    12481 ttttgtacga aggctgaccc gcctaattcc ttgatccgtt tgagacccat cgagccattg
    12541 gcgccggtgc cggataggac gacgcatacg gcctgattcc cgtgactttc cgcaagcgtc
    12601 cggaagaaga tgtcaaccgg ggcgcgccgg tcttcgggct ggaggttggg agatacggtg
    12661 atctggccgt ccagcatctc caggtgctgg ttcggcggga caacatagac atggtcgggc
    12721 cggacggcag tattgccgac gatctgtatt accggcatgg aggtcgcgac ctgcagcacc
    12781 tcggctaatt tgctgtcatg gtcgggagag agatgcaaaa tgactacgta tccgatcccc
    12841 gaattggggg gtacgttttg aaaaaatgtt ttcaatgcct gtataccgcc tgccgatgca
    12901 ccgatcccta caaaaagaaa ttcgtgtgag gcggcctttc ccttgttgtc ttcatatttg
    12961 tctgtggaga aaagaggacc cgatggatac atagattgac agctgcgggt aaaaagaata
    13021 gacggttaac tgcaagctat cgcatttggc ttgaattgcc cggaatttgt acgggtttat
    13081 tttaagggaa ctattacgag ccggcaacgc tgacccgagt ttgagggagg gtccgcagcc
    13141 cggattttat tcgtttgtta cttcgatgcg gcggcgaatt gcttcctcag gcgcgggacc
    13201 ggacttctca gcgccagttg ttgatcgata ccggcagcca tccggtcggc aggatggtat
    13261 cgccagcaaa ttaacaatga tttgatagtc agttgatccg cgtcagcgtt gtaataatct
    13321 ccgcgcaaac gcaccaggac ggtactcccg ggccgaccat cttcgaatcc gcgaaattcc
    13381 acagctgcaa cggacactac ggcgggctga gccagctgtt gaggcgtgct gccggtgagt
    13441 agtttcccga tgagagcgag ggtccggtcg agttcctccg tggctgcggt aatgtgctgc
    13501 ttcatatatt cttcatcggt cttatagctg ctgagaaaga tcctggtccg cgtttggaga
    13561 tcggcgcccg aataggtttt gcgcaactga tccagcgctt gctgttttcc ggtctcctgc
    13621 tgcgtagcgg agcgtatatt tatccggctt tttatatcgg cgatgagcat ttctttccgg
    13681 ttttccaact cctgctgcgc caggctgagg tattcttttc tggtcactgg tatatacggc
    13741 agcatgctac tgtcggctgc gaccagccag gcctttacat gcactgtctc cgtgtccgcc
    13801 gccgaattgc caccatgtgc cggcatatcg aattcaaaag ccggactgct tccggtgaac
    13861 cctttcaacg tgacgaaggc ttgaccattc accgtcaggc tgccggtcag cggactgata
    13921 tcgttggcga agatcgtgag gtcgcaggga tcgttcgggt ttacatcatc gttgtgaaat
    13981 atacagctta cgccgaaccg catctgtgga gcgccgttgc cggcggaaaa atactcgccg
    14041 tgccatatgg gatcgaatgt accctgcgtg agaatggaat cgtggaacac ggaaatgatc
    14101 ccttcgctgc tgcccttcag cttcgcgaga aggctgcggc cgatatgacc gaagacctgg
    14161 tcggcttcct ttttccaggg tgcggccgga gcagggccaa gcggacagac ttgcggttca
    14221 ttggaatttt cggtttctgc accggcggca gtgactcgat tctcagcgat aaattgagcg
    14281 cgggaaagac cgcacagggc aaacatcgac aggatgaaag cgtagatgtg tttcatgata
    14341 ggcatatttg tcaggagcgg atcgggaagg ggtccttaga atatgggaaa cagcagcggc
    14401 tttcaggagt ggatcgcgaa gagtatgagt atctgaaata acactaagat gatgccaaaa
    14461 atgcaacgca tgacccgaca aggaggcgag gaaggcagga gccgcggtct attcgctttt
    14521 ttccatcgat acggtgtgga attgcttcct taaccgcggg atcggattcc tcagggccag
    14581 ctgacgatcg ataccggcag ccatccggtc ggcagggcgg tatcgccagc atattagcaa
    14641 cgatttgata gtcgtctgat ccgtatcggc tccataccag gaaggccgca gccttaccag
    14701 caccgtactg ttaggcatgc cgtcttcgaa cccatggaaa tccgcggctg ccacggatac
    14761 gacggcaggc tgagcgagtt gccgggtagt gctgctagtg attaatcgtg cgataattgc
    14821 gagcgtgcga tcgaggtctt cggtgtttac agctatgttt tgcttcatgt actcttcatc
    14881 gctcttgtaa ttgctgagga ataccctggt atgcgcttgc agatcgatgc ccgaataggt
    14941 agcgcgcagc tgatccagcg cctgctgctt agcggcttcc tggtctgcgg agggtctgat
    15001 ctgtatcctg cttttgatat cggcaatgac ggcgtccctc cggctctcca gttcctggcg
    15061 ggcctgctcc aggtattcct ttctgctcac cgggatatag ggaagcacgc tgctgtcggc
    15121 ggtgaccagc caggccttga tatggactac gctatcgccc gcatcggccg gcatatcgaa
    15181 ttcaaatccc ggccggtcct gtccgcccgt gaaccctttg agcgtagtga attcatgacc
    15241 gttgacggta agacggccca acagcgggct gatatcgttg gcgaagatcg tgagatcgcc
    15301 cggggaattc gagttttcat ccccgttata aaacacgcag ctcaggccaa aacgcatctg
    15361 cggggcgcca ttgcccgagg agaaatattc tccgtgccag atgggcctga agctgccgtc
    15421 ggtgaggata gagtcatgga gaagggaaat gatgtcttca ctggtattct tcatcatcgc
    15481 cagccggttg ccgcggatat gtccgaagac gtgatcctct tctttctgcc agggttcgct
    15541 gatggcgggt accatcgggc aaacaagcgc ttcggtgatt ccggcgggtc cgtcggcggc
    15601 ggtgggaaca ttgtgattgt cggcgatgaa ttgggcgcgg gaatgatcgc acatggcgaa
    15661 aaccagcagg gcaatagcat aaaggcgttt catatcaggt acttttatag ggacgaaaat
    15721 tgttgctcaa cgggccggcg aaaagaccgg gttggctttc aggagtggat gctgacggga
    15781 ttcttagaat atggatctcg gggagagatc ggataataga taaatggagg aaattcccag
    15841 gataccgggt atgagatagc ttaatagtac caatgtaata ccaaaaatat aaggcgctga
    15901 aaagcaatcc gctgcaattt tttgccggca caagtcgctc tctcattggg aagctaagcg
    15961 gacgcgcatt ccgtaaacgg gagggatttt aaggaaattt tgggcttttt gcggcctcgc
    16021 cggcgaccga atccggcggc gattggatat ttttgcgcga gttttaaaat atcctaaaaa
    16081 aatcgtgatg aaagtttcga ggaccgcgcg cggtaaatac ctgcctctgc tttttttgtt
    16141 gttgatcgcc accctgagtt tcgacttcac tggtaaaacc cctattccct cccctacttc
    16201 gtctatttcg tctatttccc ctatttcgtc tgtttcggcg cgagcctccg ggttcttcgc
    16261 gctatacgac gatctgcggc tgggcgctct cgatctttcc cgggatgctt ttcaacacgc
    16321 cgtagaaggt tttctcgctc tccggaccgc aggccgtctc ggcaatcaga atgtcttgtc
    16381 tatcatcgat ttctcgttgc cttcctcgca gagacgtctg ttcatcctcg atatggccaa
    16441 cggaaggctg ctattcaaca cctttgtcgc gcatggaagg aattcgggtg agctgatggc
    16501 cacgcggttc tccaaccggc tcagcagcta tatgagcagc ctgggattct atctcaccgg
    16561 ccggtctttt atggggaagc acggctattc gttgcgcctg gaaggaatgg aaaaaggatg
    16621 gaacgaccat gccctgcaac gcgcaatcgt catgcattcc gcgccctatg tcagtgaaga
    16681 tcatatccgg aggtggggtt ccctgggacg cagcgaaggc tgcccggcta ttcccgcaaa
    16741 agtgcgcacc gcgatcatcg acgagataaa gggcggcagc tgtcttttcc tctatgcccc
    16801 ggatgaccgg tacctcgacc gggcggatcc tttctcctga ggggtacaga aattgcatcc
    16861 tctgctacaa aaacgatgaa aatgaagacg cccggagctc cggttacgct tgggagatgc
    16921 ctgcttgcag gtcttttgac aggcatcgtt gcggccgtca tcgtgtccat cttcaatatc
    16981 atttaccgga gagagactga actgatcgcg tatgcggtgg tgatgcatct gagtatattc
    17041 atggctttcc cctttttcca cctcgtttcc ggcgggttct attacctctt catcgaccat
    17101 ctgaaaaaag gcacgctgat cttcgtcgtc atcatgctgt tgggaacggt gatcagcgcg
    17161 ctgatcaccg cctatgccgg caatcggagc gatccgacgg tagaggcatt ccgtggtctg
    17221 ctgataggat tggagatcat cggcggcttg ctgggagctt tcctgatacc gtatcttgtc
    17281 aggcatccca ccctgttcct cactgccgaa gatataaaag gggaagagta gtcctgcgcg
    17341 aaatagtcct acgcgaaata gtcttacgcg atgcgccatt ggtgcacata ccgggtgagc
    17401 tcgtccgtcg ccgccctgtt gtctatccac ccgatatggc cgtcggggcg gatcagacag
    17461 atagtaccac ggtgaatggc gccggcgggg ctgaacacat gcagaggtat actactgcgg
    17521 ctacaccact ccgtaaggtc cttcgacgcc tcgccgtaac aatggatctg ccagtccatg
    17581 gattgcagga ccccgtagtt atcctgtctc atcagccatg gcagccggtc gccgccatga
    17641 ataccgtgcg ccgtcccttc gctgagagcg ctgccccgat attggatgcc cgtctgcgag
    17701 accacccgga aggccaccct cctgacggcc cttttccgga acaataccgg gaaaaggttg
    17761 ggaacgatat gagtcctgac ttccgaggcg agcgatcctc tccggttgac gaatgcaaaa
    17821 gccttgtccg tgctggctac cagccgccgg gcaaacctga tcctttccgt ctcgtaggta
    17881 tcgaggaggg catcgggcgc cccatcccgt agcacggctt ccagcttcca tgccaggttc
    17941 actgcatcgc ctatgccggt attcatgccc tgtccgccca cggggctgtg tacatgcgcg
    18001 gcatcgccta aaagaaatac gc
//
LOCUS       DASYSV010000429         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_342876, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000429 DASYSV010000000
VERSION     DASYSV010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342876"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /gene="dxr"
                     /locus_tag="VGQ51_13180"
     CDS             complement(<1..899)
                     /gene="dxr"
                     /locus_tag="VGQ51_13180"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV9037578.1"
                     /translation="MSNATVQKRIALFGSTGSIGKQALEVIDANPSVFSVEVLTAHSN
                     DDLLIRQALHFKPDTVVIVDEKKYPHVKSALSGHPIKVYTGEKALEEVAALDNYDMML
                     AAIVGFAGLRPTLSAIANGKAIALANKETLVVAGDLVMKHALDCKAPVIPVDSEHSAI
                     FQCLIGETKNRIEKIILTASGGPFLGKKPNFLVNVKRDHALQHPNWNMGAKITIDSAT
                     LMNKGLEMIEAKWLFNLQPDQVQVVIHPQSIIHSMIQFEDGSIKSQMGLPDMKLPIQY
                     ALSFPRRLASNFPRYDFRKPNTLT"
     gene            complement(906..>2100)
                     /locus_tag="VGQ51_13185"
     CDS             complement(906..>2100)
                     /locus_tag="VGQ51_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEV9037579.1"
                     /translation="GTTSGVKYIPISKESISNHINTARNALLCYMASTGNTAFSDGKM
                     IFLSGSPELERVGGVPTGRLSGIVNHHVPGYLRTNQLPSYETNCIEDWETKLDKIVEE
                     TIGQDMTLISGIPPWVQMYFDRLTELKGKPVGEIFPHFSVMVHGGVNFEPYKHRLFDS
                     IGRKVDTIETFPASEGFFAFQDSREEEGLLLNTNSGIFFEFIPAAELGKENPARLSLK
                     DVRTGENYALVVSTNAGLWGYSIGDMVRFISVEPFRLVVTGRTKHFISAFGEHVIGEE
                     VEYSLMRAAAEAGLHITEFTVAPMIQQEKGKSYHEWFVEFDGKPVDLAAFAGQVDLHL
                     REKNIYYDDLITGNILQPLKIRPVKKHGFIDYMKSVGKLGGQNKVPRLSNDRKLAAEL
                     EKYTE"
BASE COUNT          480 a          559 c          537 g          524 t
ORIGIN      
        1 gtcagcgtat tgggcttccg gaaatcatac cgcggaaagt tgctggccag acgccgggga
       61 aagctaaggg catactggat gggcagcttc atatcaggca gccccatctg agatttgatg
      121 ctcccgtcct caaactgtat catgctgtgg ataatgctct gaggatgaat aactacctga
      181 acctggtcgg gctgcagatt aaaaagccat ttagcctcga tcatctcgag acctttattc
      241 atcaatgtgg cggagtcgat ggtgatcttg gcgcccatgt tccagttggg atgctgcaag
      301 gcatggtccc tctttacatt gaccaggaaa ttgggcttct tgcccagaaa aggcccgcca
      361 gaagccgtga ggatgatctt ttcgatacgg ttcttcgtct cgcctatcaa acactgaaaa
      421 atggccgaat gctccgagtc gaccggaatg accggcgctt tacaatccag ggcgtgcttc
      481 atgacgagat cacccgcaac gaccagtgtc tccttattgg cgagtgcaat ggctttgccg
      541 tttgcgatag cgctcagcgt cgggcgcaag ccggcaaaac ccacgatcgc agccagcatc
      601 atatcataat tatccaacgc ggccacctct tcaagggctt tttcgccggt atagactttt
      661 atcgggtgac cagataacgc agatttaaca tgcggatatt tcttctcgtc gacgataacg
      721 accgtgtccg gcttgaaatg gagcgcctgt cttatcagca gatcgtcatt gctgtgtgca
      781 gtgagcacct ctacgctgaa caccgacgga ttggcatcga tgacttccag cgcctgttta
      841 ccgatggagc cggttgaacc gaaaagggca atacgttttt ggacagtggc gtttgacatg
      901 ggacgttatt cggtatattt ttccaattcg gcggctagct tgcggtcatt gctgagacgc
      961 ggcaccttat tctgtccacc cagcttcccc accgatttca tataatcgat gaaaccgtgc
     1021 tttttcactg gacggatctt cagcggctga aggatgtttc ccgttatcag gtcatcataa
     1081 tatatatttt tttcgcggag gtgcagatcg acctgcccgg caaaggcagc caggtccacc
     1141 ggcttcccgt cgaactctac gaaccattca tgataggatt tccctttctc ctgctggatc
     1201 atcggtgcca cggtgaattc ggtgatatgg agaccggctt cagctgcggc cctcatgaga
     1261 ctatactcca cctcttcgcc gatgacatgc tccccaaacg ccgaaataaa atgtttggtg
     1321 cgtcccgtaa cgaccagccg gaaaggttcc accgagatga aacggaccat gtccccgata
     1381 ctgtatcccc aaagaccggc attcgtgctg acgacgaggg cataattctc gcctgtgcgc
     1441 acatccttca gcgatagacg ggcggggttc tccttaccca gctcggcggc agggatgaat
     1501 tcgaaaaaga taccgctgtt ggtattcaat agcaatcctt cttcttccct ggaatcctgg
     1561 aaagcgaaga acccttccga ggcggggaag gtctcgatcg tatcgacctt tcttccgatg
     1621 ctgtcgaaaa gacggtgttt atatggttcg aaattgacgc cgccgtgaac catgacggag
     1681 aaatgcggga atatctctcc cacgggtttg ccctttaatt ccgtcaacct gtcgaaatac
     1741 atctgtaccc acggcgggat accgctgatc agggtcatat cctgtccgat ggtctcctct
     1801 actatcttat ccagcttggt ctcccaatct tcgatacaat tggtctcata ggaaggcaac
     1861 tggttcgtcc gcagatagcc gggaacgtga tgattgacga tgccgctcaa ccgaccggtc
     1921 gggacccctc ctaccctttc caattcaggc gaacccgaaa gaaagatcat cttaccgtcg
     1981 gaaaaggcgg tattgcccgt ggaggccata taacataaca gggcgttcct ggccgtgttg
     2041 atatggttgg aaatggattc tttcgagata gggatgtatt tgacgccgct ggtggtgccg
//
LOCUS       DASYSV010000430        12482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1619106, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000430 DASYSV010000000
VERSION     DASYSV010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12482
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619106"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGQ51_13190"
     CDS             <1..336
                     /locus_tag="VGQ51_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV9037580.1"
                     /translation="NYLSGFEKKDWNLVASQFTDDFNFTSPAGDDHISLAKYKEKCYP
                     TSKFFKKVEFPKIMVEGNTAFAIYDIHTTDNKLVHNVEYYTFRDGKIKSIECFFGVGN
                     NYPGNNKNK"
     gene            597..2105
                     /locus_tag="VGQ51_13195"
     CDS             597..2105
                     /locus_tag="VGQ51_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV9037581.1"
                     /translation="MIKATIRFSLRLQRADATGKAPIELTYQIQGERSSYQTGQSIWP
                     HFWDKEKGAAVYHEISKTKAKEIFPGVEYASLPSQRDIVKINDEMAALRIKIGEVEKR
                     LKYEFETAEKGEDKRWGPADVIAAIRAAKSPDIKPRETRESKKHVYDFIEAYIKSNLS
                     TREAGSLSVYKSLASHLKAFEDSTKRKVRFKDMDYSFFEQFQEFLLTPRSRFFPPKSS
                     KPGAVGKSKVVSLTNTTVAKQLSTLKTFIGYARKREVPVSEGYKHFKIELKKNAEVVA
                     LTEEEFQSLFNLDLKGDKRLDQVRDIFCFACATGLRYSDMAQLQWEHIKNDEIRITVK
                     KTKEWLTVPLNPYSHAILQKYKGQARPLPSNVGVKTGSLSLFSNQKMNMYLKGWADRD
                     AKTGKTEIHKGLCEIAGINEPTEVVRFKGSKREATIYPKYKLVGVHTGRKTFATLSLE
                     KGMSAEEVMTITGHKDYKSFKRYVKITEKRKKVVMRNAWGEIKKPKSKLKAV"
     gene            2119..2619
                     /locus_tag="VGQ51_13200"
     CDS             2119..2619
                     /locus_tag="VGQ51_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037582.1"
                     /translation="MKRLIATAYHEAAHAIAALEFQHKFDFVTIIPTTVTGFEILGQV
                     GGLKLAKSISNKLETGLDISMPERVELKHLLIIKLAGGAAEKRLRNLKGFPKSATEDI
                     RSSHQHSLRLFKGNYQLAKSYHENIRKEAINLVGRSDIWGKIESLAYRLMIDRSLRYD
                     DCIRIE"
     gene            2691..3059
                     /locus_tag="VGQ51_13205"
     CDS             2691..3059
                     /locus_tag="VGQ51_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037583.1"
                     /translation="MPRKSNKKITGVKAITDPAAQRKIEADYERARLKIEAQMNHLKR
                     FEELVSMKITAEHCPGCKAFLSCPNMRHGQRFNKNWCYPNGITNLKDIDHGEKLFAQF
                     AKGLTLVEDLKPAAETFSGN"
     gene            3081..3800
                     /locus_tag="VGQ51_13210"
     CDS             3081..3800
                     /locus_tag="VGQ51_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037584.1"
                     /translation="MQRKENNKGKSLNTEPETAEQKEIRLLKKRVFRAKQNAAMYEKW
                     WRDGAVALDKARKYIELLTRMEKSARDMFEITKKQWEFQQREIENLRKQRDGDADGKG
                     DGDISQEAAQGLRDMLDEGLSLQEMIDRATPEQLIIISQSQRKVLGDGLGTFEDRMKL
                     IEGFKQKAYKEILEEDSINNGPRRDIFLICVAADSDGLLDEFSRYSSPGLKRYSGCDY
                     YPINELKGKWFPAELFHKSSK"
     gene            4324..5076
                     /locus_tag="VGQ51_13215"
     CDS             4324..5076
                     /locus_tag="VGQ51_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037585.1"
                     /translation="MTEFDMEVISQLNLNKFVSWALSLKRDDCRPTPKTKDSFQAWRK
                     KSFESLSGAFCAWQEGGNPLPLHLTAGKFGRHLVESMTDKECMLVEAVVNAYFVKADF
                     ALAYLVGKLDFLQWLWNTHSAGSGGNAVVKTSPKRRTTAGNDRKYESLNSLSLRQIAL
                     LYWYNKDVIYHSTAKEILAHHVRNDERQLVRFYNSADETNERLGLNVPKSAAYRLRDL
                     QTVLPHITLKEGKDLAKIEIDRIKSYLSEQEE"
     gene            5411..5683
                     /locus_tag="VGQ51_13220"
     CDS             5411..5683
                     /locus_tag="VGQ51_13220"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV9037586.1"
                     /translation="MTVNPFEELNLRFDKLESLLAGISPVTAADPVEKPINTEELCAF
                     LGITRPTAVRYRKKGTIPFLLIGSAIRYDRRAVLKALEAKPSKRSR"
     gene            5723..6835
                     /locus_tag="VGQ51_13225"
     CDS             5723..6835
                     /locus_tag="VGQ51_13225"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV9037587.1"
                     /translation="MQLPDQKYEKKPTPEIDLRVVTGEIPPPTANPPEDIYEQKFKSS
                     ILDQAKEYPLPQAIVNFVCKGIYTPLFTAKSYSLHQGKQKSKKTTGLALMIADYIRLT
                     PSEDETRFECALPGSVLFIDTEQGESYAARTMRLILKMAGLNSSENLVYCDLREHPAA
                     DRRKIIEAGIRLTPNLKMVVIDGIVDLMTDFMDPAEGHMTITALVSLCSQYDIHIAGV
                     LHQNKGDKNARAHVGTISSQKCEMEIMTEIDPDDRSQSIVTCVNSRGLPFEPFAIRWD
                     KGSLPRINQEWNSGARQEGKTVKKVEAARAIAETVFKPLAAVSSADAIESIMKITTKS
                     KSTAKRYLEDLEGWGMVKQGPDKKYRLNMDGKNAGK"
     gene            7022..7945
                     /locus_tag="VGQ51_13230"
     CDS             7022..7945
                     /locus_tag="VGQ51_13230"
                     /inference="COORDINATES: protein motif:HMM:NF020379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BT4734/BF3469 family protein"
                     /protein_id="HEV9037588.1"
                     /translation="MNHKMNWMNKQVSLYVSHADNIGRPATVGEILLTEFASFRKWKG
                     DRLNDMETIVEIRNLDRSAPDYRNRKLALKATLQCYTPAGLLVTKEHGKMALIHWSGL
                     LQLDFDQAGIADFDLEELKQCVFALPFIAFCGLSCSGDGFYALACIAEPERLADYAEH
                     LFLVLEDYGIKADQSKGKKVENLRYVSYDSNMLIRDNPEILRVTNFRTKPAPQKSHSA
                     TLRAPIAGHSALVRSELAKIAGAVVNQRWDTVQRVAFTLGGLGDSSLIYAIKAEIEAN
                     PEFNGQEAKYIKCAQDCFAAGAQKPLSHATH"
     gene            7932..8261
                     /locus_tag="VGQ51_13235"
     CDS             7932..8261
                     /locus_tag="VGQ51_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037589.1"
                     /translation="MQRINLTNIQSVNIVAIPSETITGREFRYSNIARIRTRDGGDYD
                     ILFDERGEPVTDFGLMERVGKHYGKKFQAIVYDGRAAYAEINGNSDKSVQKQGPQARS
                     KIAVAKG"
     gene            8329..8691
                     /locus_tag="VGQ51_13240"
     CDS             8329..8691
                     /locus_tag="VGQ51_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037590.1"
                     /translation="MVAKKVVNKQKAIQNMAKFQPGVSGNKAGKPAGVSRKQKLLNAF
                     AQDIVSGGTAKFKRELNSLKGRSYVEAYLTLLEYVQPKLARVEHLGDGNNMQIITVKL
                     EDEATGSNTMAILGEDHQ"
     gene            8639..10093
                     /locus_tag="VGQ51_13245"
     CDS             8639..10093
                     /locus_tag="VGQ51_13245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV9037591.1"
                     /translation="MKRPVQIQWPSWEKIINKAFLPLIGCRDRYLILYGGRGSSKSDF
                     TAKKLIFRCLTEKYFKAILVRNAYNSIKDSSYQTIRDIIVDMGLQELFEFKLNPLEIV
                     CRNGNTFLARGCDDTTRLKSIKDPSAVWYEEDIPTEPDWITISSGVRTRKAEYLQEIF
                     SLNPECEGDYQLSWFWKRFFEGKEGLSFQDKTMVEVKPGVTVELAVTVHHSTYKDNRW
                     ISMPFVAAMEDLKRTDSYYYQVYCLGRWGQRVRATAAYKQFAMGRNTAPVCYNKELPL
                     WVSFDFNVLPGVSCGIFQVEGKIMRMIGEIQLPPPRNNTKAVCKEIIRLYQGHIGGMM
                     VTGDPAGRHKDTRGEDSYNDFDIIIGSLAQFRPSLRVHTSAPPVVPRMNFINTVFESG
                     YNGISLTIGSHCEKMIADLLNGQEDGDGTKRKEKVRDPKTGASIERYHHFTDLLDYAV
                     ITVFYNEFKKYLTGGGPSFPTRFGRNAPNKKLAY"
     gene            10093..10410
                     /locus_tag="VGQ51_13250"
     CDS             10093..10410
                     /locus_tag="VGQ51_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037592.1"
                     /translation="MIPTKIPSRYFFRTTGAIEIDTGDFLLIANHLGFRTRGGRIYRL
                     VTDSRQVIRSSRTAYFVAMRDLLSDDLPDLADVLYAIDEVIARYQDQLIYGLEKLESF
                     YNV"
     gene            10442..10975
                     /locus_tag="VGQ51_13255"
     CDS             10442..10975
                     /locus_tag="VGQ51_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037593.1"
                     /translation="MLTEVEQMQAKALGATTAKINAEKVQAKAAVEKKDKPATFHQKE
                     LVRKNTMTDEKMKAAETAGHCKATGFFGKLHEPAHPEHSAVLSEFKNKYGGSEAKARA
                     GLMADAMREYDCHPIENRDTSFEAKTLEQNRPKLNASKTDLAHYDILKDSLIAEWAAR
                     GYSGTDDVIKNWKAKYK"
     gene            11261..11551
                     /locus_tag="VGQ51_13260"
     CDS             11261..11551
                     /locus_tag="VGQ51_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037594.1"
                     /translation="MRDSIWGTVDHDRGESNCKPTKKLGMAEDFEFRMPEDAVILSMK
                     DYQDLTNAAQPSLYQKALGEIIALIIKIEPEGEIAKGASDILTFHQIHLKPI"
     gene            11628..>12482
                     /locus_tag="VGQ51_13265"
     CDS             11628..>12482
                     /locus_tag="VGQ51_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037595.1"
                     /translation="MNFDFVHSFECSDNWRDLTNKGKLTMPKAIFYRNQYGAKKPLFG
                     TNVNVGGFDSNAPLVLRGDAVTIASGYKYFTTGNRELTDTSTMFTGYISKVRSKIPIE
                     IELEDNMWLVKQTPMDLVTFSKSDTLEHVLQVVINKVNAVWGTQLTVNNTTATTVGQS
                     FAIGNEQGAQVLLKLHKLYGFESYFRGNELRSGILVYLPADTVTADGTPKTPNIFAFQ
                     QNIIDDKLEYVRKDDINLSAKAINTVTEETGELTKSGAAKTRRKRLEVLVTIKNNPRP
                     GDLPYTFSI"
BASE COUNT         3698 a         2952 c         3170 g         2662 t
ORIGIN      
        1 aactatttat caggttttga aaagaaggac tggaatttag ttgccagtca atttactgat
       61 gactttaatt tcaccagccc agccggagat gatcacatat ccctcgcgaa gtataaggag
      121 aaatgttacc caacgtctaa atttttcaaa aaagtcgagt tccccaaaat tatggtagag
      181 ggcaacacgg ccttcgctat ttacgatatc catacaacag acaacaagct tgtccacaat
      241 gttgaatact acacttttag agatggcaag ataaagtcta tcgagtgttt ttttggtgtc
      301 ggcaataatt accctggtaa taataaaaat aaatagagaa atcaccttcc tgcaccccca
      361 ccaaaaaaca aaaccgcaac catttgctct acaaataatt gcggttatat tctgcggaga
      421 gggcgggatt cgaacccaag cgtttatagt taacaatagt ccacgctcat tagcatcata
      481 tctttgtctt acagtcactt atgcaaaccc gcgtaaacgc atgaaaatat atgttgcaca
      541 aaagttgcaa ccaaagccgc aaccaagtag ttttgggaga gatatatttt cgaaccatga
      601 tcaaggcaac cattcgtttt tctctccgcc tccaacgtgc tgacgcgacc ggtaaggcac
      661 ccattgaatt aacctatcaa atacagggcg agcgaagcag ttaccagacc ggtcagtcga
      721 tctggccgca cttttgggac aaggagaaag gagcagccgt ataccacgag ataagcaaga
      781 cgaaggcaaa ggagattttt ccaggcgttg aatacgcctc ccttcccagc cagcgggaca
      841 ttgtcaagat aaacgacgag atggccgcct tgcgcataaa aattggcgag gtagagaagc
      901 ggcttaagta tgaatttgag accgccgaaa aaggcgaaga taagcgatgg gggccggctg
      961 atgtgattgc agccatccgc gccgccaagt cgccggatat caaaccgagg gagacaaggg
     1021 aatcaaaaaa acatgtgtac gacttcattg aagcctatat aaaatccaat ctatctacca
     1081 gggaggccgg gagcttgtcg gtatataaat cgcttgcaag tcacctaaag gctttcgagg
     1141 actccaccaa aagaaaggtc cggtttaaag acatggacta ttcctttttc gagcagtttc
     1201 aagagttcct gctcacaccc cgcagtcgct ttttcccacc gaaatcttcc aagcctggag
     1261 cagtcggcaa aagcaaagtt gtatcgctca ccaataccac cgtcgcaaag cagctttcta
     1321 cgttaaagac gtttatcggc tacgctagga aaagagaagt gcccgtatca gaggggtaca
     1381 agcacttcaa aatcgaactt aagaaaaatg ccgaggtggt ggccttgacg gaggaagagt
     1441 tccaaagcct cttcaacctt gacctaaagg gtgacaagcg gctggaccag gtcagggata
     1501 tcttttgctt tgcgtgcgct acaggcctca gatattccga catggcgcag ctgcaatggg
     1561 agcatataaa aaatgatgag atccgcataa cggtgaaaaa aacgaaggaa tggcttacgg
     1621 taccccttaa tccctattcc cacgccatcc tgcagaagta taaagggcaa gcccgccccc
     1681 tgccttcaaa cgtgggggtt aaaacggggt ccctatcgct attcagcaat caaaaaatga
     1741 atatgtatct gaagggctgg gccgaccggg acgcaaagac cgggaagaca gaaattcaca
     1801 agggtttgtg tgaaatagcc gggatcaatg agccgactga agtagtccgg tttaagggct
     1861 ctaagcgcga agcgaccatt tacccgaagt acaagctcgt cggtgtccat accggaagaa
     1921 agacctttgc cacgctgtcc ctggaaaaag gcatgagcgc ggaggaggtt atgacgatca
     1981 ccggccacaa ggactataag agctttaagc ggtacgtcaa gatcaccgaa aaacgcaaaa
     2041 aggtcgttat gcgcaacgcc tggggtgaga tcaaaaagcc gaagtcaaaa ctcaaagcgg
     2101 tataaaatgg caaggcaaat gaaacgattg atagctaccg cgtaccacga ggcggcgcat
     2161 gcaatagctg ctcttgaatt tcagcataaa tttgactttg tgaccattat ccccacgacc
     2221 gtcaccggat ttgaaatact cggacaagtt ggagggctga aattggcgaa aagtatctcg
     2281 aataaactag aaaccggttt ggatatttct atgccggaaa gggtagaatt aaagcatctc
     2341 ttaattatta aactcgctgg aggagcggca gaaaaacgcc ttcgaaacct gaagggcttt
     2401 ccgaaaagtg ccacggaaga tattcgatct tcgcaccagc attccctcag actttttaag
     2461 gggaactacc agcttgcaaa aagttaccat gaaaatattc gaaaggaggc aattaacctg
     2521 gttggtcgaa gtgacatttg gggtaagatt gaatctttag cgtaccggct tatgattgat
     2581 aggtcactca gatatgacga ttgcattaga atcgaataag gcaccttgta aacaaaaaac
     2641 ccgctggctg taaccggcgg gcattattca acataaaacc gtgaagtatt atgccacgca
     2701 aaagtaacaa aaaaatcact ggcgtcaaag ccattactga tccagccgct caaaggaaaa
     2761 tcgaggccga ttacgaacgg gcccgtctta agatcgaggc ccaaatgaac cacctcaaaa
     2821 ggttcgagga attagtttcc atgaagatta ccgccgaaca ttgcccaggg tgcaaggcgt
     2881 tcttgtcctg cccaaatatg cgccatggcc agaggttcaa taaaaactgg tgctatccta
     2941 atggtataac caatttgaaa gacatcgatc acggcgaaaa actcttcgcg cagttcgcca
     3001 aaggcctcac ccttgttgag gatcttaagc cagctgcaga aacgttctcc ggaaattaat
     3061 aaccgttcaa acctaaattt atgcaacgca aggaaaataa caaaggtaaa tcgctgaaca
     3121 ccgagccgga aacggcggag caaaaggaaa ttcgtctctt aaagaaaagg gtctttcgcg
     3181 caaaacaaaa cgcagctatg tacgaaaaat ggtggaggga tggagctgtg gcactcgata
     3241 aagcaaggaa gtatatagag ctgctgaccc gtatggaaaa aagcgcaagg gatatgtttg
     3301 aaattaccaa aaagcaatgg gaatttcaac agcgagaaat tgagaatctc agaaaacaga
     3361 gggatggtga tgcggacggg aaaggggatg gcgatatcag ccaagaggcc gcgcagggat
     3421 tgcgggacat gctcgatgaa gggttaagcc ttcaggaaat gatcgatcgg gcaacgccag
     3481 agcaactgat tattatttct cagtcgcagc gaaaagtact aggcgacggt ctcggcactt
     3541 tcgaggatcg catgaaacta atagaaggat ttaagcagaa ggcctataag gaaatcctgg
     3601 aagaagacag catcaataat ggtcctcgcc gggatatatt tcttatctgt gttgctgccg
     3661 attctgatgg cctattagat gagttcagcc gatattcctc gcccggattg aaaaggtata
     3721 gcggatgcga ctactatcct attaacgagc tcaaagggaa atggtttccc gcagagctgt
     3781 ttcataaatc ctcaaaataa gcgcatgcac caaatatgca aaaactgtcc cctgggcaga
     3841 tgcggtttcg atggtattaa ggtatatgcg gcaatccaag cctgccaatt cattcccatt
     3901 ggtaaaggca tacgatatct gcaaacgctt tcgaaagccg aacaaaatga aatagtgtcc
     3961 gacggcaggc tgcccggcaa gggagacatg agtttactag agtctcggtg gaaatatcgg
     4021 acaatcatta ccggtaagaa cgagaaggct gaggacactc cggagacgat agcgcggcag
     4081 gccgtgttcg gatcggacag cagacggcta aatatgaact aaaccttttt cacacacaaa
     4141 ccatcaacca tgaaaaaaga caatgttatt actgtcctgc tcacgtctgc aatccgatcc
     4201 gcatcggaaa gaattatgcc actgccgcgg ctctgcctgc gggagacgcc aagaaacaaa
     4261 cgagcaaaga aatagacacc acagcggggc cggcatatgg tcccgctttt ttaaatttgt
     4321 gatatgacgg aattcgatat ggaagtaatc tctcagctta atctgaataa gttcgtcagt
     4381 tgggctctga gcctgaaacg ggacgactgc aggcctacgc cgaagacaaa agacagcttt
     4441 caggcctgga gaaagaaatc cttcgaaagc ctatccggcg ctttctgcgc atggcaggaa
     4501 ggcggtaatc ctctgcctct gcatttgaca gctggtaagt ttggaaggca ccttgtggaa
     4561 tccatgacgg ataaggaatg catgctggtc gaagccgtag taaacgcata ttttgtcaag
     4621 gcagatttcg ccctggcata cttggtggga aagctggact ttttgcaatg gctgtggaat
     4681 actcattcgg ctggctcggg gggtaatgct gtggtcaaaa cgtctcccaa aaggaggaca
     4741 acagcaggaa acgaccgcaa atatgaaagt ttaaactctc tttcccttcg gcaaattgcg
     4801 ctcttatact ggtacaacaa agatgtgata tatcattcaa ccgcgaagga aatattagct
     4861 catcacgtaa gaaatgatga gcggcaactt gtaaggtttt ataactccgc ggacgaaacc
     4921 aacgagcgcc ttggtctcaa cgtgccaaag agtgccgcct atcgcttacg ggaccttcaa
     4981 accgttctcc cgcatatcac ccttaaagag ggaaaagact tggcaaaaat tgaaatcgac
     5041 aggataaaat cgtatttgtc ggagcaggaa gagtaatgat ccctccgcga accctcacac
     5101 aatctttcct gattatttcg gttaaacccc ctacagttgg gggttttcaa ttttgtgtga
     5161 gggttgtgtg agggttgtgt gagagatgga agaatcgata cgtctaaatt ggtcttataa
     5221 atttcaactt catgaccaat gacgagaaac taagggcggt tcgaaagagg ttgtctgaaa
     5281 ttgactcttt gctttggagc atacagcccg gccgcggcaa caaccgcgaa ttggctcggc
     5341 ggcaatccgt aaaaaacctt gcgtcccaaa gggcagcggc aaacgagggt accgggaaag
     5401 gaggtaagaa atgaccgtta acccctttga ggagcttaac ctccgattcg acaagctgga
     5461 aagtctgctt gccgggatct cacccgttac agctgccgat ccggtcgaga agccgatcaa
     5521 cacggaggag ctatgcgcgt tcctgggcat cacccgacct acagcggtac gataccgaaa
     5581 gaagggaacc ataccctttc tgctgatcgg cagcgcgatc cggtacgacc gccgggcggt
     5641 attaaaggcc ttggaagcga aaccatcaaa gcgaagtcgg taaaaaaata tcccagcggc
     5701 caggctggga caataaaatc caatgcaatt accggatcaa aaatacgaaa agaagccaac
     5761 accggaaata gacttaaggg ttgtcaccgg agaaatacct ccgccgactg caaatccgcc
     5821 ggaggatatc tatgagcaaa aatttaaatc atccatttta gatcaggcca aagaatatcc
     5881 gctacctcag gcaattgtca attttgtctg caaagggatc tatacgccgc tcttcactgc
     5941 gaaatcatac agcctgcatc agggcaagca gaagtctaaa aaaacgactg ggctcgcctt
     6001 gatgattgcc gactatattc gacttacgcc gtcggaagac gaaaccaggt ttgaatgcgc
     6061 gctacctggc tcggtcctgt tcattgatac cgagcaggga gaaagctatg ctgcgcggac
     6121 gatgcgcctg attcttaaga tggccggttt gaattcttcg gagaacctgg tctattgcga
     6181 cctccgagaa catccggccg ccgaccggcg aaagatcatc gaggccggca tccggctcac
     6241 tccgaaccta aaaatggtag tgattgacgg gatagtggat ctcatgacgg atttcatgga
     6301 ccccgccgag gggcatatga ccatcacagc cctggttagc ctttgctcac aatacgatat
     6361 ccacattgcc ggggtgctgc atcagaacaa gggggacaaa aacgccaggg cgcacgttgg
     6421 gaccatatcg tcccaaaagt gcgagatgga gatcatgaca gagattgacc cggatgaccg
     6481 ttcgcaaagc atcgtcacat gtgttaatag ccgtgggctg ccatttgaac cgttcgccat
     6541 ccggtgggat aaagggtcgc taccaagaat taaccaggaa tggaattccg gcgcccggca
     6601 ggagggaaag acggttaaga aggtcgaagc cgcacgggcg atagcggaga ccgtcttcaa
     6661 gccgctggca gctgtatcgt ccgccgacgc gattgaatcg attatgaaga taaccaccaa
     6721 atcaaagagc acggcaaagc ggtatttgga ggatttggaa ggttggggta tggtgaagca
     6781 aggcccggat aagaaatatc gccttaacat ggatggtaaa aatgcgggga agtgatgcgg
     6841 gttcataaaa gtcaaaaccc atgttttgaa ctttttgaac caagcgataa aacagggaag
     6901 ggtcaaaaag ttcaaaaagt ccaaaatgac ttttgaaccc tgttcaaaaa agttcattca
     6961 tccacttata tataagtggt gaaaatgaac ctccgatttt gactcaaaaa aaagcaggta
     7021 aatgaaccac aaaatgaact ggatgaacaa acaggtatct ctttacgtca gtcacgcgga
     7081 caacatcggg cggccggcaa ccgtcggcga aatcctgctc actgagtttg cctcattcag
     7141 gaaatggaaa ggggaccggc tcaatgacat ggagacgatc gtcgagatcc gcaacctgga
     7201 tcgatcggcg cctgactata gaaaccggaa actagccctg aaagctaccc ttcagtgcta
     7261 tactcctgcc ggtcttctgg ttactaaaga acacgggaag atggctttga ttcattggtc
     7321 cgggctttta cagctggatt tcgaccaggc aggtatagcc gacttcgacc tggaggaatt
     7381 gaagcaatgc gtctttgcgc tgccttttat cgctttctgt ggcctcagct gtagcggcga
     7441 cggattttat gccctggcct gcatcgccga gcctgagcgc ctcgcagact acgcagagca
     7501 ccttttcctc gtcctggaag actacggcat taaagcagat caatccaagg gtaagaaggt
     7561 cgaaaacctg cggtatgtgt cttatgactc caatatgcta atcagggata acccagagat
     7621 cctgcgggtc accaactttc ggacgaagcc agccccgcag aaatcgcact ccgcaacttt
     7681 gcgggctccg atcgcaggcc actccgctct tgtgcgttca gaattggcca aaatagcggg
     7741 tgccgttgta aatcaacgct gggacaccgt ccaacgcgtt gcatttacgc tcggcggatt
     7801 aggcgactca tccctcatct atgccatcaa ggccgaaata gaagcgaatc cggagttcaa
     7861 cggacaggag gccaaataca tcaaatgcgc ccaggattgc tttgcagcgg gcgcacaaaa
     7921 accactatcc catgcaacgc attaacctga ctaatatcca aagcgtaaat attgtcgcaa
     7981 tcccatccga aacgatcacc ggccgggaat tccggtacag caacattgcc cggatcagga
     8041 ccagggacgg cggagattac gatatccttt ttgatgaacg cggtgagccc gttaccgact
     8101 tcggacttat ggagagggta gggaagcatt acgggaagaa gtttcaagcg atcgtttacg
     8161 acggcagggc cgcttatgcg gagatcaatg gtaattctga taaatcagtc caaaaacagg
     8221 ggccgcaggc gcgaagcaaa atcgctgtag cgaaaggtta ggttcgaatc cgccgcactc
     8281 ttgtaaacag tagtaaaatc agcccttgca gaggtaaaat aagggcgaat ggtagccaaa
     8341 aaggtagtaa ataaacagaa agcgatacag aacatggcaa agtttcaacc aggagtttca
     8401 ggaaataaag caggtaagcc ggcgggagtt agcaggaagc aaaagctact gaacgcattc
     8461 gcccaggata ttgtcagcgg cgggacggct aagttcaaaa gagagctgaa cagcctgaaa
     8521 gggcgaagct atgtcgaggc atatttgacc ctgctcgaat atgttcagcc gaaacttgcc
     8581 agggtagagc accttggcga tggaaataac atgcagatca ttaccgtaaa actcgaagat
     8641 gaagcgaccg gttcaaatac aatggccatc ctgggagaag atcatcaata aggcgtttct
     8701 cccgctcatt ggctgccggg acaggtattt aatcctgtac ggcggccggg gcagcagcaa
     8761 aagcgacttc acggcaaaga agttgatctt tcgctgcctg acggagaagt actttaaagc
     8821 cattctggtg cgcaatgcct acaattcgat taaggatagc agctaccaga cgatccggga
     8881 tatcatcgtc gatatgggcc tgcaagagct ctttgaattc aaactcaatc cactggagat
     8941 cgtttgccgc aatggaaaca cattcctggc aaggggttgt gatgatacaa cccggctgaa
     9001 gtcaataaaa gacccatcgg ctgtctggta tgaggaagat attccaacgg agcccgattg
     9061 gatcactata tccagtggag tccgcacccg taaggccgag tacctgcaag agatcttcag
     9121 tctcaaccct gagtgcgagg gcgactacca gttgagctgg ttctggaaac gcttctttga
     9181 gggcaaggaa ggcctgtcat tccaggataa gacaatggtc gaggtcaagc ctggcgttac
     9241 ggtggaactg gctgtaacgg tccaccactc gacttacaag gataaccgct ggatatccat
     9301 gccatttgtc gcggcgatgg aggacctgaa gcggacggac tcttattatt accaagtcta
     9361 ctgcctgggc cggtgggggc agcgcgtccg ggctacggca gcctacaagc aattcgcgat
     9421 gggccggaac acggcgccgg tatgctataa caaagagctt cccttgtggg tcagcttcga
     9481 ttttaacgtg cttccgggcg tttcctgcgg catcttccag gtcgaaggca agataatgcg
     9541 catgatcggg gaaatccaat taccgccgcc caggaacaat accaaagcgg tctgcaaaga
     9601 gatcatccgg ctataccagg gccatatcgg gggaatgatg gttacgggcg acccggcagg
     9661 ccggcacaag gacacgcgcg gcgaggattc ttataatgac ttcgatatca tcataggctc
     9721 acttgctcaa ttccggccat cattgcgcgt gcacacttcg gctccgccgg tcgttccgcg
     9781 catgaatttc ataaacaccg tctttgaatc gggatacaat gggatcagtc tcacgatcgg
     9841 cagccactgc gaaaagatga tcgccgactt gctcaacggt caggaggatg gcgacggcac
     9901 gaagcgaaaa gagaaagtcc gggacccgaa gaccggggca tcgatcgaac ggtatcatca
     9961 ttttaccgac ttgctggatt acgcggtgat caccgtgttc tataatgaat tcaaaaaata
    10021 ccttaccgga ggcggcccgt cttttccgac aaggttcgga cgcaacgcac ctaataaaaa
    10081 acttgcttac taatgatacc cacaaaaata ccgtcgagat acttttttag gacgaccggc
    10141 gcgatcgaga tcgataccgg ggacttttta ctgatcgcca accacctggg ctttcggact
    10201 cgcggcggcc gaatttatcg gctcgtgacc gactcccggc aggtgatccg gtcaagcagg
    10261 acggcctatt tcgtcgccat gcgcgatttg ctgtccgatg atctgccgga cctcgcggat
    10321 gtgttgtatg cgattgacga agttatcgcg cggtatcagg atcaattgat ctatgggctt
    10381 gaaaagctcg aaagttttta taacgtgtaa acccatttct tcatcaataa aacgaaatac
    10441 aatgttaacg gaagtagaac aaatgcaggc caaggcacta ggcgcgacga ctgcaaaaat
    10501 aaacgcggag aaagtgcagg caaaggctgc cgtcgaaaag aaagacaaac ccgccacctt
    10561 tcatcaaaag gaattggtaa gaaaaaacac aatgaccgac gaaaagatga aggcggcaga
    10621 gacggccggt cattgcaaag cgaccggatt ctttgggaag ttgcatgagc cggcgcatcc
    10681 cgaacactcc gccgtgctga gcgagtttaa gaataaatat ggcggcagcg aagcaaaagc
    10741 gcgggcagga ttaatggccg acgccatgcg ggagtacgat tgccatccga ttgagaaccg
    10801 ggacacgtct ttcgaagcaa agaccctgga gcagaaccgg ccgaagttga acgctagcaa
    10861 aacggatctg gcccactacg atattctgaa agacagcctg atcgccgaat gggcagccag
    10921 gggctactcc gggacggacg acgttatcaa aaattggaaa gccaagtaca aataagtatc
    10981 agaaaagccg gagcggtatc gtcccgcttt gactaaacat ccatattgga gccgggggag
    11041 tttcgtcctc ccctggattt ttaaatagaa gtatgaacaa gaaccaaaag gcctttctta
    11101 aaaccttact ccgatccgtc gagcaagcgg aaagggcgcg ggattcaatt ctcggatccg
    11161 tcgacgaagg cctgatcacg ggctcacacc ggcagcaatg tatcgagtat tgccgggcgc
    11221 tacggcggta tattgacagt agccggacaa tcgcgaccga atgcgtgaca gcatatgggg
    11281 aaccgtcgac cacgatcgcg gcgaaagcaa ttgcaaacca acaaaaaaat taggtatggc
    11341 agaggacttt gaatttagaa tgccggaaga tgcggtaata ctttcaatga aagattacca
    11401 ggatctcacc aatgcagcgc aaccttcctt gtatcagaaa gcattaggcg aaatcatcgc
    11461 gctgatcatc aagattgagc cggaaggcga gattgcaaag ggtgcatccg atatccttac
    11521 attccatcaa attcacttga agccaattta ggaccatgta ccgtatagtt acgcaaataa
    11581 ccattcagca aaggccttcc cccgattggc caaaccggaa aattcagttg aatttcgact
    11641 ttgtacactc ttttgagtgt tcggataatt ggcgggatct cacgaacaaa ggaaagctga
    11701 ctatgccgaa agccattttt tacaggaacc agtacggcgc aaaaaagcca ttgttcggga
    11761 caaatgtgaa tgttggcggc ttcgactcaa acgctccgct tgttctcagg ggcgatgcgg
    11821 taacgattgc gtccggctat aagtatttca cgaccggtaa ccgggagctc accgatacgt
    11881 cgacaatgtt taccggctat atcagtaagg tacgctctaa aatacctatt gaaattgagc
    11941 tggaggacaa tatgtggttg gtaaagcaaa cgccgatgga cctggttact ttttcgaaat
    12001 ccgacaccct ggaacacgtc ctgcaagttg tgatcaacaa agtaaacgcg gtttggggca
    12061 cccaactaac ggtaaataac accacggcga cgaccgtagg ccaaagtttt gctatcggca
    12121 atgagcaagg cgcccaggtc ctgttaaagc tgcataaatt atacgggttc gagagctact
    12181 tccgaggtaa cgaactacgg tccgggatac ttgtctatct gccggctgac acggtgaccg
    12241 ccgacgggac acccaaaaca ccgaatatct ttgccttcca gcaaaatatt atcgacgaca
    12301 agctcgaata cgttaggaaa gatgatataa acctcagcgc gaaggcgata aacaccgtca
    12361 cagaagaaac aggcgaattg accaaaagcg gcgcagcaaa gacccggaga aagcgccttg
    12421 aagtcctggt gacgatcaaa aataatccac ggcccggtga tcttccttac accttcagca
    12481 tt
//
LOCUS       DASYSV010000431        13641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_87109, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000431 DASYSV010000000
VERSION     DASYSV010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13641
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87109"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="VGQ51_13270"
     CDS             complement(<1..1510)
                     /locus_tag="VGQ51_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037596.1"
                     /translation="MAANFFKITLRHLFKKQKGFTFINVIGLTAGLTSCILIGLYITD
                     ELSYDNFHSRADRIARVTWEFSVGGTVSHYALTGTKVGPQFKRTFPGVEEYTRTFIWP
                     ANLSHGNDHFEEKKFLYADSSFFSVFSFPLLAGDPHPLRSKEDIVLTASAARKYFGTT
                     DVVGKTLTVNDDKQFTISAVAKDPPRNSQIHFDFVISFNNLDASKHEQWRTANYVTYL
                     LLHHPSDIGPLQKGIDAYVKTPQVRKEAGCEGSDFIRYYLEPLKSVHLHSPYSGYEPN
                     GNITYVYVLMLIAVLILTIACVNYTNLAIAQSSARTGEIGIRKVLGALRTQLFSQFAG
                     ESLFTTLIALLLAVGASIILLPYFNALTGKQLIPSDILQARSLAAIAAAGIGIGLLAG
                     AYPALVLANTRLIAILRSGFRITGGHAGLRRTLIVLQFVISLFLIITTVVILQQMSYI
                     RNRNLGLDRDHVLVLPIDYRILDRYDGLKTAVKTLPGVINVSGSYNLPISATW"
     gene            1580..3961
                     /locus_tag="VGQ51_13275"
     CDS             1580..3961
                     /locus_tag="VGQ51_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9037597.1"
                     /translation="MLKNYFTVAWRNLLRSKWYSLINTLGLSIGLAVALLIGLWIWDE
                     LSFDHYYENHSRVAQVMTTQTFNGRTGTGEATSLPVGKTLRTQFAGDFSEVARCSWTF
                     DHTLAVGDRMITQMGMYAEPGFSHIITLRMVKGSRDALKDPSAILLTQSAATALFGNE
                     DPMNKTVKIDNLAVAKVEGIFEDPPRNTSFSSVKFLLTWAMYESTADWVRHAEDQWGN
                     HSWPTFVLMAPKADPARVTARIKDLPKAHNKEGDETLLLQPMDNWHLYSDFKDGKVSG
                     GLIRFVWLFAIIGVFVLLLACINFMNLSTARSEKRAKEVGIRKTVGSLRTQLIGQFLC
                     ESIVVALLAFVLAIGWVLLALPSFNTLADKDMHLPWANPVFWVLTLGFTLVTGLLAGS
                     YPAFYLSGFDPIRVLKGAFRAGRFASLPRKVLVVLQFTVSVALIIGTILVFSQINYAR
                     NRPVGYSREGLISIMMNTPEIFGHYNALRGDLLRTGAVADMCESSSPITNVWSNQIGW
                     DWKGKDPQFVPLFGTIACTHDYGKTLDWSIKEGRDFSRNFVTDSGGRGGLILNEAAVK
                     LTGMSHPLGETITWNDSPRVVIGIVRDMVMENPYQPIKPTIFFISYGWTNVIDVRLNP
                     SMSARAALRAIAPVFKRYNPGSAFEYKFVDEEYARKFTDEQRIGRLATVFAVLAIFIS
                     CLGLFGLASFVAEQRTREIGVRKVLGASLFHLWGMLSADFVLLVVISCAIAVPVAWYF
                     LHQWLKQYEYRTPMYWWAFVGASLGALAITLLTVSYQAVRAGMANPVKALRSE"
     gene            4043..4654
                     /gene="pnuC"
                     /locus_tag="VGQ51_13280"
     CDS             4043..4654
                     /gene="pnuC"
                     /locus_tag="VGQ51_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV9037598.1"
                     /translation="MQFDELWHSLRQEAADIGWLQWISTVFGVAEVLLARVNNILLYP
                     AGIISSLIAIFLLFDTKLYADAGLNVYYVVMSIYGWWFWSRKNDEPAPGIGWTTRSEW
                     LVVIGIAAGGFAVLYILLARFTPSTTPVWDAWVTATAYAGTWLLARRKIENWILLNVS
                     NLFAVPLLYYKQLHLFAALTVFLFIVACFGYFDWRRRYKLQIA"
     gene            4651..5721
                     /locus_tag="VGQ51_13285"
     CDS             4651..5721
                     /locus_tag="VGQ51_13285"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV9037599.1"
                     /translation="MTALSTPAIDILRGYAAVAEKAGRLQPEQLALIYEQGWFRMMVP
                     RRYGGLALPLPEVVRLEEAISRADGSAGWTVTLCSGAGWFSGFFPPSALAGLFSDARL
                     CIAGSGTPSGRAQLIPGGYRVSGKWSYASGAHHATAFTANCTVWSDDIQVMTDAGNPV
                     VQPFLFLREEVRIVEDWKAMGMVATGSHGFEVHGLELPAERGFIIDASAAADDDPLYR
                     YPFLPLAEATLAVNSCGMMLHFLDCCTSVFGERIGRRQIPVRMSAEMMNALDSAREEI
                     ALRRDEFYSALDESWATMQPGSYHRVSRTSHALALAVRQWSDRLFPYAGLGAARVDSE
                     INRVWRDLHTAGQHPLLVFDSR"
     gene            complement(5753..6142)
                     /locus_tag="VGQ51_13290"
     CDS             complement(5753..6142)
                     /locus_tag="VGQ51_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9037600.1"
                     /translation="MLVYTSVFSSYSVSDLAKAKEFYEKTLGFPVTQTPEGLRVQVSK
                     DYTVFLYPKPDHQPATFTVLNLQVMSVEAAVDALTAAGVKFEHYEGAIATDAKGIMHG
                     GPGKGPTIAWFTDPARNIISVVENPPV"
     gene            6319..7083
                     /locus_tag="VGQ51_13295"
     CDS             6319..7083
                     /locus_tag="VGQ51_13295"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEV9037601.1"
                     /translation="MQNYKRFVLGTGLALVAGAFFVGWRWHPAQGGFAVVELFTSEGC
                     SSCPPAEALMAQTQQEDKDLPVYCLAFHVDYWDHQGWKDVYSNAAYTDRQRQYSRWLR
                     LNEMYTPQAVVNGTTEFVGSNGDEMTRAIRTGLGQKETVSLTLTGLRLNAGRLDWQYK
                     IDGLAAAAGRRLTLVTAVVEKNAVTQVKGGENSGRTLSQAQIVRNLGMQVVGADGSGS
                     GHIDWPAGLRVGDGEVIAFLQDQDNGKIIAATRVKP"
     gene            complement(7117..8604)
                     /locus_tag="VGQ51_13300"
     CDS             complement(7117..8604)
                     /locus_tag="VGQ51_13300"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9037602.1"
                     /translation="MKKLKYISPILVLALLTVSCHREDLTPQSQTLVGNNSAFSTPAR
                     IYNQVIGLYGALKAGGLYGGRFQIAGDVKADNFIDELNNLVTDQDVWTGNPTNSATAV
                     VNQWSAAYLAINNCNVFIEGMNETGTKMVGATLGNQYISEAKVIRAICYYGLLQFWAN
                     AYTKDGGASLGVPLRITAITAPGSSNQIRSKVADCYTQIVKDLTDAEAGLPSSYSATA
                     TNNAAYWNATRATVNTATALLTRVYLTMGNWAGVISEANKIVSATAPFTAPSGVAFAL
                     QSDVTKVFTAPYTTTENILFLPMSSTSGDNPGTQNQMGYYFSPSKGVVSGGTGNGEFS
                     LNPNGVISDPTWKSTDRRWAFIATSTSGGKKWCNKFPAPSPYTDYTPVIRYSEVLLNL
                     AEARTRSTNSVDPQAVALLNAVRQRSDATTTFAPASVSALIADILQERNIEFLGEGLR
                     NNDLVRLQMPIPTKGSAPAKNPGDQGYIWPISANELTLNTLCVDN"
     gene            complement(8637..11834)
                     /locus_tag="VGQ51_13305"
     CDS             complement(8637..11834)
                     /locus_tag="VGQ51_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9037603.1"
                     /translation="MRKLLAHLLCLCLAFSQIEAQQPTSTTVSGTVTDEKGAPLAAVT
                     VTALTGDRKVTVTTVTDVNGVFKINVTPRVRTLQFTYIGLEEQFIPLGGKTAFAVTLR
                     ANNRNLSEVVVVGYGTQQKKDVTASITTVKGAAIAATPVQSFEQGLAGRAAGVQITVP
                     SGIVNEPPVFRIRGTNSISLGSYPLIVIDGVVSFENDQGSTNAPANPLASVNPSDIES
                     IDIAKDPAATAIYGSRGANGVVFITTKKGRAGRMRVSYDGWVGNASVYGLPHMMNAAQ
                     YIAYKSQAVANNPTLGSSGITFNYAKDANGNNVDTKWFDHIYQTGHQQSHALSIAGGN
                     DNTTYYVSFGYTDENGIIRKDEFKRYNVLANVDSKVNKILTVGAKLSYSDEYNLAATS
                     SGALPGEAFNTGGLGRIGMVLPSILPVYNPDGSYNINGSTVGYANITGTSISYYNPQP
                     LIDHSRSNNENNHVQSNIYAQLKPLPWMTLKTAYGIDNLWIDNDFFATPITGDGYSAT
                     GQAVTNLRKLKTWVWDNTIQLDHTFGENHNFSLLAGNEQQRIMTSGYGVNRTTLADPA
                     FSTEQATYTTTNSANTALSQSYLLSTFGRLTYNYNHKYFVEGTARRDEYSAFAVKGGN
                     FYAGSVGWELAQENFWKTTKLDHVFSSFRIRGSYGKVGNSAGLGDFASLSFYGSSVVG
                     GNPSLGFYNGSVGNPNLTWETSKQTDVGFNFGIMNDRITGQLAYYNNNVNGLILFVAQ
                     APSTGLPSSPPQNIGTMYNRGIEVTISANVVQSKSFSWNTSLNFTTNTNKVTSLAAGL
                     PSLQTGTSGLETVNLTKPGYSEGYLWVIRTAGVDAGTGTRVFVNQQGTKVYYRYAVPS
                     GQGMFNYSTTPDGKTKYVGPSSTSITQAADAVMYKNTLPKYVGGWSNTFNYSHFTLDV
                     LLTYQAGFYVYYGSNAGLHDQRFWNNATDVLTDAWSQAKGQKNAKYALPMFNDNVSNG
                     SAMPMDMNVFKGDFIKLRNVSLSYNMPNSITQAMKISSLRVYVSGQNLGIMSKYPGPD
                     PEVASNGNTSNSGQGVDRNTIANARTITVGLNVGF"
     gene            complement(12057..12989)
                     /locus_tag="VGQ51_13310"
     CDS             complement(12057..12989)
                     /locus_tag="VGQ51_13310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV9037604.1"
                     /translation="MNKTFLLLPVLLLAQITLRAQRLIQVGPSQDSKGNYVFSCTNND
                     YCTYILYLDFPTLTNAKSDHALPYEAEVKPGLNKLLTISPVDPSKDIKFNFKTSSRKG
                     CLHPVVNANFIYLLPIRPGLETQAYRVVNSRNGAGGTGGTGSAGGAGGTGSPDSGYAI
                     RLRASIGDTIYAARRGVVTVVAVSNTENDAGANTTDSWNHVEIAQADCSFAEYGVLKK
                     DGAFVKPGQVVEAGTPIGLVGGDRYGRGSDVRFSVSYYPGTPGTGIQLQFWTKGNGKG
                     PLKHGATYTSEFPPNILLQEKTKPAAKKPRPRAR"
     gene            13121..>13641
                     /locus_tag="VGQ51_13315"
     CDS             13121..>13641
                     /locus_tag="VGQ51_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV9037605.1"
                     /translation="MSSFIHKFYKRWQRWKSDVPVSPREANRHPSAGETEQRLIAEQL
                     DRQKAFYENILNKLPTDIAVFDADHRYLFVNPGAISKEEYRKYIIGKDDYEYAAYRNR
                     DVSVAHSRRAKFMEAKQTGKEIRWEDTLTDPQGNMITHLRRMFPVFDQTGELSLVIGF
                     GIDITDRKVMEVK"
BASE COUNT         2887 a         3366 c         4175 g         3213 t
ORIGIN      
        1 cccatgtggc ggaaatgggc aggttgtacg aacccgagac gttgatgacg cccggaaggg
       61 tttttacggc tgttttcaga ccgtcgtaac ggtcgaggat ccggtagtcg atgggcagga
      121 caagaacatg atcccggtcg agcccgagat ttctgttccg gatatagctc atctgttgaa
      181 ggatgacgac ggtggtgatg atcaggaaca gcgatatgac gaattgcagg acgatgagcg
      241 tccggcgcag accggcatgt ccgccggtga tacggaaccc cgatcggagg atggcgatca
      301 gtcgggtatt ggcgagcaca agggcggggt aagcgcccgc gagcagtccg ataccgatgc
      361 ctgctgcggc gatagccgcg agcgacctgg cctgcaggat atccgagggg atcagctgtt
      421 tgccggtcaa ggcgttgaag tagggcaaca ggatgatcga agcgcctacg gccagcagca
      481 gggcgataag cgtggtaaaa agggactcgc cggcgaattg ggaaaagagt tgtgtcctca
      541 gtgctccaag gacctttcgg atgccgatct cacccgtgcg ggcggaagac tgggcgatgg
      601 cgagattggt ataattgacg caggcgatgg tcaggatcag aacggcgatc aacatcagga
      661 catagacgta ggtgatattg ccgttgggct catagccgga gtaaggggaa tgcagatgaa
      721 cgcttttcag cggctcgaga taatagcgga taaaatcgct gccttcgcaa ccggcttcct
      781 tgcggacctg gggggtcttt acgtaggcat cgatgccttt ttggagcgga ccgatatcgg
      841 agggatgatg gagaagaaga taggtgacat aattggccgt tcgccattgt tcgtgtttgg
      901 acgcgtcgag attgttgaag ctgatgacga aatcgaaatg gatctgtgag ttgcggggcg
      961 ggtccttggc aacggcgctg atggtaaatt gtttatcgtc attgactgtg agcgtcttgc
     1021 cgaccacatc ggtcgtaccg aaatatttcc gggcggcgga ggcggtaaga acgatatcct
     1081 ccttgcttct cagcggatgg gggtcccccg ccagcagcgg aaacgaaaaa acggagaaga
     1141 atgaggagtc cgcatagaga aatttctttt cctcaaaatg atcgttccca tggctgaggt
     1201 tcgccggcca gatgaaggtc cgggtatact cttccacccc tggaaaggtc cttttaaact
     1261 gcgggccgac cttcgtgcct gtgagcgcat agtggcttac cgtgccccca accgagaatt
     1321 cccaggttac gcgcgcgatc cggtcggcgc gggagtggaa gttgtcgtag ctcagctcgt
     1381 cggtaatata cagaccgatg aggatacagg aggtgagtcc ggcggtcagc ccgatcacat
     1441 tgataaaagt gaatcccttt tgttttttga agagatgacg gagggtgatt ttgaagaaat
     1501 tggcagccat atacaaatag gacgcacgga atgcaacaat tgttgcatgt ccttgtcctt
     1561 ttttgcacaa accggatata tgctcaaaaa ctatttcact gtcgcctggc gaaacctgct
     1621 ccggagcaaa tggtattcgt tgatcaatac cctgggattg tccatcggcc tggccgtcgc
     1681 cctgctcatc gggctatgga tctgggacga gctgtccttt gaccattatt atgaaaatca
     1741 ttcgagagtg gcgcaggtga tgacgaccca aaccttcaac ggtcggacgg ggacagggga
     1801 ggcgaccagc cttccggtag gcaaaacgct gcggactcag tttgccgggg acttttccga
     1861 ggtggccagg tgcagctgga cgttcgatca taccctggct gtgggcgaca ggatgatcac
     1921 ccagatgggc atgtatgccg agccgggctt ttcccatatc attaccctgc ggatggtcaa
     1981 aggcagcaga gatgccctta aagacccttc ggccatcctc ctcacccaaa gtgcggcgac
     2041 ggccctgttc ggcaacgaag atcccatgaa caaaacagtg aaaatagata acctggccgt
     2101 tgcgaaggta gagggtatct tcgaggaccc gccccgcaac accagtttta gttccgtgaa
     2161 attcctgctg acctgggcta tgtatgaaag caccgccgat tgggtgcggc acgccgagga
     2221 ccaatggggg aaccattcct ggccgacctt cgtcctgatg gcgccaaagg cagatcccgc
     2281 acgcgtcacg gccaggatca aagacctgcc caaggcgcat aataaagagg gggatgaaac
     2341 cctgctcctc cagccgatgg acaactggca tttgtacagc gacttcaaag acgggaaggt
     2401 atcgggcggt ctgatccggt ttgtgtggct gttcgcgatc atcggggttt ttgtgctgtt
     2461 gctggcctgt atcaacttta tgaatctttc cacggcgagg agcgagaaaa gggccaagga
     2521 agtgggcatc cgcaagacgg tcggatcatt gcgtacgcaa ctgatcggcc agttcctttg
     2581 cgaatcgatc gtcgtcgccc tgctggcctt tgtgcttgcc atcggctggg tcctgctggc
     2641 gctgccgtcg ttcaatacgc tggccgataa ggacatgcat ctgccgtggg cgaatccggt
     2701 tttctgggtc ctgacgcttg gtttcacgtt ggttacgggg ttgctggcgg ggagctatcc
     2761 ggcgttttat ctgtcggggt tcgaccctat tcgcgtgctg aaaggggctt ttcgggccgg
     2821 ccggtttgcc tcgctgccga gaaaggtgct ggtcgtcctg cagttcaccg tttctgtcgc
     2881 gctcatcata gggacgatac tggtcttttc acagattaat tatgcgcgga accgaccggt
     2941 tgggtatagc cgggaagggt tgatcagcat catgatgaat accccggaga tcttcgggca
     3001 ttacaacgcg ctgcgcggtg acttgctgag gaccggggca gtcgccgata tgtgtgagtc
     3061 gtcgagccct atcacgaatg tatggtccaa ccagatcggt tgggactgga aggggaagga
     3121 ccctcaattc gtcccccttt tcggcaccat cgcctgcacg catgattatg gcaaaaccct
     3181 ggactggtcg atcaaagagg gccgcgattt ctcgcgcaat tttgtgaccg acagcggagg
     3241 caggggaggt ctgatcctca acgaggccgc ggtgaaactg accgggatgt ctcatcccct
     3301 gggggagacc attacatgga acgactcacc gcgtgtcgtc atcggtattg tccgggatat
     3361 ggtcatggaa aatccatatc aacccatcaa gcccacgatc ttttttatca gttatggctg
     3421 gaccaatgtg atcgacgtcc ggttgaaccc gtcgatgtct gcgcgtgctg cgctacgtgc
     3481 gatcgcgccg gtgttcaaac gatacaatcc gggtagcgcg ttcgaatata agttcgtgga
     3541 cgaggagtat gcgcgcaaat tcacggatga acagcgtatc gggagactgg cgacggtgtt
     3601 tgcggtactg gccatcttta tttcctgtct gggtcttttc ggtctggcct cttttgtcgc
     3661 cgagcagcgg acgcgcgaga tcggcgtccg caaggtactg ggcgcctcgc tgttccattt
     3721 gtgggggatg ctttcggcgg attttgtgct gctggtggtc atttcgtgtg cgatcgccgt
     3781 tccggtcgcc tggtattttc tgcatcaatg gctgaaacaa tatgagtacc ggacgccgat
     3841 gtactggtgg gcgtttgtgg gtgcctcgct gggcgcgctg gcgatcaccc tgctgacggt
     3901 gagttatcag gcggtgaggg cgggaatggc gaacccggtg aaggcattgc gtagcgaatg
     3961 agccactctg cggcttcagg tctgcgaact gagtgagatc gtgtttgttc gcacgcggcg
     4021 gggattatgt atcttgccgc ccatgcaatt cgatgagctt tggcattcac ttcggcagga
     4081 agcggcggac atcggctggc tgcaatggat atccacggtg ttcggcgtgg cggaagtact
     4141 gctggccagg gtcaacaata tccttttata tcctgcaggg ataatcagca gcctcattgc
     4201 gatctttctg ttgttcgaca cgaagcttta tgcggatgcc gggctcaatg tgtattatgt
     4261 cgtcatgagt atctatggct ggtggttttg gagcaggaaa aatgacgaac cggcgcccgg
     4321 gatcggttgg accacccgca gcgagtggct ggttgtgatc gggatcgctg cgggtggttt
     4381 tgcagtactg tatatactgc tcgcgcggtt caccccttcg accacgcccg tctgggacgc
     4441 ctgggtgacg gctacggcgt atgccggcac gtggctgctc gcgcggagga aaatagagaa
     4501 ctggatcctg ttgaatgtct cgaatctatt cgccgttccg ctgctatatt acaaacagct
     4561 gcacctgttc gctgcgctga cggtcttcct cttcatcgtc gcctgtttcg gctatttcga
     4621 ctggcggcga cggtataaac ttcagatcgc atgactgcgc tgtccacccc ggccatcgat
     4681 attttgcgcg gctatgctgc cgtcgcggag aaggccggcc gtctccaacc cgaacagctg
     4741 gcactgatat atgaacaggg atggttccgg atgatggtac cgcgcaggta tggggggctc
     4801 gcgcttccgt tgccggaagt agtgcggctc gaggaagcca ttagcagggc agatggttct
     4861 gcgggctgga cggtcacttt atgcagcggc gcgggttggt tttccggatt tttccctccg
     4921 tcagcgctag ccggtctgtt ttcggatgcc cgtttgtgta tcgcgggtag cgggacacct
     4981 tccgggcggg cgcagctgat ccccggcgga taccgggtca gcggcaaatg gagctatgcc
     5041 agcggggcgc atcatgctac cgcctttacg gccaattgta ccgtctggtc tgacgacata
     5101 caggtgatga cggatgcggg taatccggtc gtccagcctt ttttgttttt gcgcgaagaa
     5161 gtacgcatcg tcgaagactg gaaggccatg ggcatggtgg cgaccggaag ccacgggttc
     5221 gaggtgcatg gtctcgagct gcccgccgaa cggggattta tcatcgatgc ctccgcggcc
     5281 gcggacgatg atcctttgta caggtatcct tttctgccgt tggcagaggc gacgcttgcg
     5341 gtgaacagct gcggaatgat gctccatttt ctcgattgct gtacttctgt gtttggcgag
     5401 cgcatcggga gaagacagat cccggtccgt atgtccgcgg aaatgatgaa cgcgctggat
     5461 tcggcgcgcg aagaaatcgc tctgagacgg gacgaatttt attccgccct tgatgaatca
     5521 tgggccacga tgcagcccgg gtcgtaccat cgggtcagcc gcaccagtca tgcactggcg
     5581 ctcgcagtgc ggcagtggag cgacaggctg tttccctatg ccggcctggg cgccgcgcgg
     5641 gtggattccg agatcaaccg cgtatggcgg gatttgcata ccgcaggcca gcatcctttg
     5701 ctggtgttcg atagccggta gcgccacctt cgatgtcagg atgccggtag cgtcagaccg
     5761 gcggattttc aaccacggat ataatattcc tggccggatc ggtgaaccag gcgatggttg
     5821 gaccctttcc cgggccgcca tgcataattc ctttggcatc ggtggctatt gccccttcat
     5881 aatgctcgaa tttaacgccg gcggcggtca gcgcatcgac ggctgcctcc acgctcatga
     5941 cctgcagatt cagcacggtg aaggtagcgg gctggtgatc gggtttggga tagaggaaca
     6001 ccgtatagtc tttcgagacc tgcacacgga gtccttccgg cgtctgggtt acgggaaacc
     6061 ccaatgtctt ctcataaaat tctttggcct ttgcgagatc gctgacggaa tagctgctga
     6121 atacgctggt gtagacaagc ataagactga attttagata cagttacagc caaaggcgtg
     6181 cctgaacgaa gttgtaccaa tttgttattc ttatgaatgg cgtggggaga gggaatattc
     6241 gcgaaattgg ggggaagaag aggaatggag cgagcatcgg aatattaccg aaattcggtt
     6301 tattaaaatt ggtgcgatat gcaaaactat aaaaggtttg tgttgggaac gggtctggcg
     6361 ctggtggcag gggccttttt tgtcggctgg aggtggcatc ccgcacaggg cgggttcgcc
     6421 gtagtggagc tcttcacgtc cgaagggtgt tcgagctgtc cgcccgccga agcgctgatg
     6481 gcgcagacgc agcaggaaga caaggacctg ccggtatact gtctggcctt tcatgtggac
     6541 tattgggacc atcagggttg gaaagatgtc tacagtaatg cggcctatac ggaccgccag
     6601 cgtcagtatt cccggtggct gcggctaaat gagatgtata cgccgcaggc agtggtcaat
     6661 ggtactaccg aatttgtcgg gtcgaatgga gacgagatga ccagggcgat ccggacaggc
     6721 ttagggcaga aggagaccgt ctcattgacg ctgaccggac tacgtctcaa tgcaggtcgt
     6781 ctcgattggc aatataagat cgacgggctg gcggcggccg ccggtaggcg tctgaccctg
     6841 gtgacggccg tggtcgagaa aaatgccgtg acgcaggtga aaggagggga gaacagtggc
     6901 aggactctgt cacaggccca gatcgttcgc aatctcggca tgcaggtggt gggcgcggat
     6961 ggctccgggt cggggcatat cgactggccg gcagggctga gggtggggga tggagaggtg
     7021 atcgcctttt tgcaggacca ggataatggg aagatcattg ctgcgacgag agtgaaacca
     7081 taaaaaaagg gccccgcgag gcggggcccg atggcattaa ttgtctacac aaagtgtgtt
     7141 tagcgtcaat tcgtttgccg atatcggcca gatataaccc tgatcgcctg ggtttttcgc
     7201 aggcgccgat cccttggtgg ggatgggcat ctggaggcgg acgaggtcgt tattccgcaa
     7261 tccttcgccg aggaactcga tattgcgttc ctgcaggatg tcggcgataa gtgccgaaac
     7321 gctggccggc gcaaaggtcg tggtggcatc cgaacgctgg cgaacagcat tgagcagggc
     7381 gaccgcctgg ggatcgaccg aattggtgct gcgtgtacgg gcttcggcca ggttaagcag
     7441 tacttcggaa taccggataa cgggtgtata gtcagtatag gggctcggcg ccgggaattt
     7501 attgcaccat tttttgccac cgctggtgga ggtggcgatg aaggcccatc tcctgtccgt
     7561 ggatttccag gtgggatcgg aaatgacgcc attggggttc agtgaaaatt ctccattgcc
     7621 cgtgccgccg ctgacgacgc ccttcgaggg ggagaaataa taacccatct ggttctgcgt
     7681 gcccgggtta tcaccggaag tggagctcat cggcaggaac aggatgtttt cggttgttgt
     7741 gtagggcgcg gtgaacacct ttgtaacatc cgattgcagt gcgaacgcaa cgccggacgg
     7801 ggcggtgaac ggagcggtag ctgaaacgat cttattggcc tcactgatca caccagccca
     7861 attccccatg gtgagataga ccctggtcag caacgccgtg gcggtgttga cggtcgccct
     7921 ggtggcattc caatacgcgg cattgttggt ggccgttgca gaataagaag agggcagccc
     7981 ggcttcggca tcggtcaggt ccttgacgat ctgggtatag caatccgcga ccttactccg
     8041 gatctgattg gatgaaccgg gtgctgtgat ggcggtaatc cgcaacggga cgccgagact
     8101 ggcgccgccg tccttcgtgt aggcgttcgc ccagaactgc agcagaccgt agtaacaaat
     8161 ggcgcggatg accttggctt cggagatgta ttggttaccc agcgtagcac cgaccatttt
     8221 ggtgccggtc tcgttcatgc cttcgataaa tacattgcag ttgttgatgg ccagataagc
     8281 agcggaccat tggttgacga cggcggtcgc gctgttggtc ggattcccgg tccagacatc
     8341 ctgatcggtg acgaggttgt tcagctcgtc gataaaattg tcggccttga catcgccggc
     8401 aatttggaaa cgtcctccat agaggccgcc cgccttgagg gcgccgtaga ggccgatcac
     8461 ctgattataa atccgggcgg gggtggaaaa agcgctgtta tttcccacca gcgtttggga
     8521 ttgtggagtc aggtcctcac ggtggcagga gacggtcaga agcgctagta ccagaatggg
     8581 agaaatatat tttaattttt tcattttatt gttttttgag ttcaacactg ttttcattag
     8641 aagcctacgt tgagaccgac ggtgatggtc cttgcatttg cgatggtatt gcggtcgacg
     8701 ccctgaccgg aattggatgt attaccgttt gaggctactt caggatcagg tccgggatat
     8761 ttggacatga tgcccaggtt ctgaccgctg acatagacgc gaaggctgct gatcttcata
     8821 gcctgcgtga tgctattcgg catattgtag ctcagcgaga cgtttctcag cttgataaaa
     8881 tcgcctttga atacgttcat gtccatcggc atggccgagc cgttcgagac gttgtcgttg
     8941 aacatcggca aggcatactt ggcattcttc tggccctttg cctgggacca ggcatcggtc
     9001 agcacatcgg tggcattgtt ccagaagcgt tgatcatgca ggccggcgtt agacccatag
     9061 taaacataga atcccgcctg ataggtcagc agtacatcga gcgtgaaatg gctgtaatta
     9121 aaggtattcg accagccgcc gacatatttc ggcagcgtgt tcttatacat cacggcatcc
     9181 gctgcctggg tgatcgaagt actggagggg cctacgtatt tggtcttgcc gtccggggta
     9241 gtggagtagt tgaacatgcc ctgaccggaa gggactgcgt aacggtaata gaccttcgtg
     9301 ccttgttggt tgacaaagac gcgggttccg gtaccggcgt cgacaccagc tgtccggatg
     9361 acccaaagat agccttcgga atagcccggt ttggtcaggt tgaccgtctc gaggcctgag
     9421 gtgcccgtct ggagggaggg aagaccggcc gccagcgagg tcaccttgtt cgtattggtc
     9481 gtgaagttga ggctggtgtt ccagctgaac gatttgcttt ggacgacatt ggcgctgatc
     9541 gtgacctcga taccccgatt gtacatcgta ccgatgtttt gaggcgggct gctgggcaac
     9601 cccgtggagg gcgcctgtgc aacgaagagg atcagaccgt ttacattgtt gttgtaatat
     9661 gccagttgac ccgtgatccg atcgttcatt atgccgaaat tgaaaccgac atccgtctgt
     9721 ttgctggtct cccaggtgag gttcgggttg cctacgctac cgttgtaaaa accgaggctg
     9781 ggattgccgc cgacgacgct cgagccgtag aaggaaagcg aagcgaagtc gccgagaccc
     9841 gcactattgc cgaccttgcc atagctgcct ctaatacgga aactgctgaa gacgtgatcc
     9901 agtttggttg tcttccagaa attctcctgt gcgagctccc aaccgacaga accggcgtag
     9961 aaattaccgc ctttgactgc gaaagcggaa tattcatccc tacgcgccgt cccttccacg
    10021 aaatatttgt ggttgtaatt gtaggtaaga cgaccgaaag tagacaaaag atagctctgg
    10081 ctcagtgcgg tattagccga gttggtcgta gtataagtag cctgttcggt cgaaaacgcc
    10141 gggtctgcca gggttgtgcg gttcaccccg tagccagagg tcatgatccg ttgctgctca
    10201 ttaccggcta acagggagaa gttatggttc tcgccaaagg tatgatcgag ctggatggtg
    10261 ttgtcccaaa cccaggtttt cagtttgcgg aggttggtga ccgcctgacc cgtggcggaa
    10321 taaccgtcac ccgtgatcgg ggtagcgaag aaatcgttgt cgatccacag gttatcgatg
    10381 ccataggccg ttttcaatgt catccaggga aggggtttca gttgagcata gatgttcgac
    10441 tgaacgtggt tgttctcgtt gttggaacgg ctgtggtcga tgaggggctg cggattgtaa
    10501 taggagatgc tggtaccggt gatattggca tagccgacag tggaaccatt gatattgtag
    10561 gacccgtccg gattatatac cggcaggatg gaaggcaata ccatgccgat acggcccaga
    10621 ccgccggtat tgaacgcctc gccaggcagg gcacccgatg aggttgcggc aaggttgtac
    10681 tcatccgagt aggagagttt ggcgccgact gtcaggatct tgttgacctt ggaatccaca
    10741 tttgcgagta cattataccg tttgaactcg tctttgcgaa tgatgccgtt ctcatcggtg
    10801 tagccgaagg atacataata ggtagtgttg tcgttgccgc cggctatgct gagcgcatgg
    10861 ctctgctggt gaccggtctg gtagatatgg tcgaaccatt tcgtgtctac gttatttccg
    10921 ttggcgtctt tggcgtagtt gaaagtgatg ccgctggagc ccagcgtggg gttattcgca
    10981 accgcctggg atttataggc gatatattgt gcggcattca tcatatgcgg caaaccataa
    11041 acgctggcat tgccgaccca tccgtcatag ctaacccgca tacgtcctgc ccggcctttc
    11101 ttggtcgtga tgaaaacgac gccatttgcg ccgcgcgaac cgtaaatggc ggtggcggca
    11161 gggtctttgg cgatgtcgat gctctcgatg tcggaagggt tgacgcttgc caaagggttg
    11221 gcgggagcgt ttgtggaacc ctggtcgttc tcaaaggata ctactccgtc gataacgatc
    11281 agcgggtagg aacccagtga gatagagttg gtacccctga tacggaaaac cgggggttcg
    11341 ttcacgatgc cgctgggaac cgtgatctgc acacccgctg cgcgacctgc cagaccctgt
    11401 tcaaagctct ggaccggcgt tgccgcgatg gcggcgcctt ttaccgtcgt gatgctggcg
    11461 gtgacgtcct ttttttgctg tgtaccataa cccacgacca ctacctcgga gagattgcgg
    11521 ttgttggcgc ggagggtcac ggcaaaggcg gttttacctc cgagcggaat gaactgttcc
    11581 tccagcccga tataggtgaa ctggagtgtg cgaacgcggg gtgtgacatt gatcttgaaa
    11641 acaccgttga cgtctgtaac ggtggtgacg gttacctttc tgtctccagt aagcgccgta
    11701 acggtgacgg ccgccagggg tgcgcccttt tcgtcggtga cagttccgga aacagtggtt
    11761 gatgttggtt gttgtgcctc aatttgactg aatgccagac ataggcataa cagatgagcc
    11821 aggagctttc tcatgtgtga gtagtagttt tggtgatata aagtcagtct gtctagcgct
    11881 aaatttacac cttcgacccc gaaatatgca atttttaccg ggaatatttc ttgattccca
    11941 ttaaaagccc ttcccgattt tgggatatct gttccggaat tccactttcg ccgaatcgtc
    12001 atcggctcgt tatcggggtg cgggtttttg cgatcacttc actgcagtgt gcgctcttac
    12061 cgggcgcgcg gtctcggttt ttttgcagcg ggcttcgttt tctcctgcag aagaatgttg
    12121 ggcgggaatt cgctggtata tgtcgcaccg tgtttgagcg ggcctttgcc attgcccttt
    12181 gtccagaatt gcaactgaat cccggtgccc ggtgttcccg ggtaatagct gacgctgaac
    12241 cggacatccg agccacggcc ataacgatcc ccgccgacaa gcccgatggg tgtccccgct
    12301 tctactacct gccccggttt gacaaaggcg ccgtctttct tcagaacgcc atattcggca
    12361 aatgaacaat cggcctgtgc gatctcgaca tgattccagc tatcggtggt attggcaccg
    12421 gcgtcatttt cggtattgga gacggcgacc acggtaacga caccacgacg cgccgcataa
    12481 atagtatcgc cgatgctggc ccgaagccgt atggcataac cgctgtcggg ggagcccgtg
    12541 ccacccgcgc caccggcaga acccgtgcca cccgtgccac ccgcgccatt gcggctattc
    12601 acgaccctgt atgcctgggt ctccaatccc ggccgtatgg gcagcagata gataaagttc
    12661 gcattgacca ccggatggag acagcctttc cgggagctgg tcttaaaatt gaacttgata
    12721 tccttcgagg gatcgaccgg tgaaatggtc agcagtttgt tgagacccgg ctttacctcg
    12781 gcctcatagg gaagggcatg atccgatttc gcattggtca gggtcgggaa gtcaagatag
    12841 agtatatagg tgcagtaatc gttattggtg cagctaaaga cataattgcc tttgctgtcc
    12901 tggctggggc caacttgtat gagacgctgg gcccggaggg tgatttgcgc caggagcagg
    12961 accggtaaga gaaggaatgt cttattcatt tgtccaattt aggcaatcac ccgtcaataa
    13021 caaacccggc gcaataccct gacgaatatt acgttacaag tagatatccc aaaccctgta
    13081 gggctgagtg attaccatgt tgataaacaa agtgtaatgc atgagttcat ttatacataa
    13141 attctataaa aggtggcaga gatggaaatc cgatgtgccg gtgagtccaa gagaggccaa
    13201 tcgccacccg agtgcggggg agaccgagca aagactcatt gcggaacaac tggacaggca
    13261 aaaggccttt tatgagaata tcctgaacaa actgcccaca gatatagccg ttttcgacgc
    13321 ggatcaccgt tatcttttcg tcaatccggg cgccatcagc aaagaggaat accggaagta
    13381 catcatcggc aaggacgatt atgaatatgc cgcttaccgc aacagggatg tctcggtggc
    13441 gcattcccgc agggcgaagt tcatggaggc aaagcagacg ggtaaggaga taagatggga
    13501 agacacgctg accgatccgc agggtaatat gatcacgcat ttgagaagga tgtttcccgt
    13561 attcgatcaa acgggggagc tatctctggt catcggtttc ggcatcgaca tcaccgatcg
    13621 taaggtgatg gaagtaaaac a
//
LOCUS       DASYSV010000432         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2265113, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000432 DASYSV010000000
VERSION     DASYSV010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2265113"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VGQ51_13320"
     CDS             <1..1468
                     /locus_tag="VGQ51_13320"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV9037606.1"
                     /translation="EWDWGGLPDYLLKIPDIKVRCRDPRYMSAVSRYVARLSKEIAPL
                     QCDRGGPILMMQLENEYGSYGNDKGYLADLRGLWRRHGITVPFYTADGPTDFMLDAGS
                     LDSCAIGLDSGTGDDDFAVATRHNPNVPVFSSETYPGWLTHWKEPWQHPDTTDIMKGL
                     RYLLSHHRSFNLYVVHGGTNFGFTAGANAFTPTQFQPDVTSYDYDAPIDEQGRATPKY
                     YALRRLIGEYAGHPLPPVPAPVPVMEIPAFTMQPYTSLWDHLPAARHTAQPEPMEYFD
                     QNNGMILYRTRLIGHKSGKLTITEPHDYAQVFLNGVFIDSIFRDGGNWTVRLPKTDVK
                     DPVLEILVESMGHINFAQYLIDRKGITDRVTLDGMTLMDWETFNFPLNAPPAVPAGAT
                     TAHTPIAIRPPGSNRGLFFRGNFNLDSTADTYFDLSGFKKGVIWINGHNLGRYWYVGP
                     QHHLYCPAPWLKKGQNEVVVLDLQQRESTTVRGVKTLE"
     gene            1490..>1861
                     /locus_tag="VGQ51_13325"
     CDS             1490..>1861
                     /locus_tag="VGQ51_13325"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV9037607.1"
                     /translation="MDSLTHIVLGAAIGELLAGRRLGKKALLIGAIAQSLPDIDFLAD
                     FWLDTARDVWAHRGITHSFLFILVMSPLLAWGARRIFRRSGMAFGDWCLFFGLQLFVH
                     VFIDAFNAYGTGWFEPFDHARI"
BASE COUNT          387 a          493 c          559 g          422 t
ORIGIN      
        1 ggaatgggat tggggaggtc tgccggacta cttgctgaag atacccgata tcaaagtacg
       61 atgcagggat ccccgttata tgagcgccgt gtcacgttat gttgcccggc tgtcaaagga
      121 gatcgcgcca ttgcaatgcg atcgcggcgg cccgatcctg atgatgcagc tggaaaatga
      181 atacggcagt tatggcaatg acaagggcta tctggccgat ctgcgtggct tgtggcgccg
      241 gcatggaata accgtcccgt tctataccgc cgacggtccc accgatttca tgctcgatgc
      301 gggaagtctg gatagttgcg cgatcgggct cgatagtggt accggcgatg acgattttgc
      361 cgtagccacc cggcataacc cgaatgtgcc tgtcttcagc agcgagacct atccaggctg
      421 gcttacgcac tggaaggaac cctggcagca cccggatacg acggacatca tgaaggggtt
      481 gcggtattta ttgtcccacc atcggtcctt caatctttat gtggtgcatg gcggcactaa
      541 tttcggattc acggcgggtg ccaatgcgtt tacgccgaca cagttccagc cggatgtcac
      601 cagctatgac tatgatgcac cgatcgatga gcagggcagg gctacgccga aatattacgc
      661 gctgaggcgg ctgatcgggg aatatgccgg ccatccgcta ccgccggtgc ccgcgccggt
      721 acccgtgatg gagataccgg cctttacgat gcagccttac acttctttgt gggaccatct
      781 tccggccgcc aggcataccg cacagcctga gcccatggaa tatttcgatc aaaacaatgg
      841 aatgatcctc taccgcaccc ggctgatcgg gcataagagc ggcaagctga ccattacgga
      901 accacatgat tatgcgcagg tgttcctcaa cggcgtcttc atcgacagta tcttccggga
      961 tggtgggaac tggacggtca ggctgccgaa gacggacgtg aaggacccgg ttctcgagat
     1021 cctcgtcgaa agtatggggc atatcaactt cgcgcaatat ctcatcgacc gaaaaggtat
     1081 cacggaccgg gttaccctgg atgggatgac gctgatggat tgggagacct tcaactttcc
     1141 gttgaatgca ccgccggctg ttccggccgg cgccactacg gcccatacgc ccatcgcaat
     1201 ccgaccgccc ggttcgaaca ggggcttgtt ttttcggggg aactttaacc tggacagcac
     1261 tgcggacacg tatttcgatc tcagcggatt taaaaaaggg gtcatctgga tcaacgggca
     1321 taacctgggg cggtattggt atgtggggcc tcagcatcat ttatattgcc cggcgccgtg
     1381 gttgaagaag gggcagaatg aggtagtggt gctggacctg caacagcggg aaagcacgac
     1441 ggtgcgcggg gtgaagacgt tggaatgagg attgctggat cagccgaaaa tggactcgct
     1501 tacccatatc gtccttggcg ccgcgatcgg tgaattgctg gctggccgcc gattggggaa
     1561 aaaagccctg ttgataggcg cgatcgcgca aagcctgccg gatatcgatt ttttggccga
     1621 cttctggctg gacactgcgc gggacgtatg ggcgcatcgc ggtattaccc actcctttct
     1681 ctttattctg gtgatgtcgc ctctgctggc gtggggagcg cgacggattt ttagacgaag
     1741 cgggatggcc ttcggggact ggtgtctctt tttcggactc cagcttttcg tgcatgtctt
     1801 catcgacgcc ttcaatgcct atggcaccgg ctggttcgaa cctttcgatc atgcgcgcat
     1861 t
//
LOCUS       DASYSV010000433         8443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2391626, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000433 DASYSV010000000
VERSION     DASYSV010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8443
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2391626"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VGQ51_13330"
     CDS             complement(<1..1584)
                     /locus_tag="VGQ51_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9037608.1"
                     /translation="MNQNSLLYPNAYRRLLVILSLVCFSGAVFGQSRQITGKVVGNES
                     DSAIVGATVSVKGSKIGTTTGQDGTFTLSVPANATLIVSYVGFTPQEIRIGTQSSFNV
                     RLAPENQSIQQVVVIGYGTQKRKDLTGAISSVSAEQIEKVPTPTLEQAMQGRAAGVQI
                     TQNDASPGGNINVLIRGIGSLANNGNSPLYVVDGYPLETGGINNINPNDIATIDILKD
                     ASATAIYGMRAANGVVIITTKRGKRSGAQISFDMYEAFQGKPKEYKVLNAEQFGTLAN
                     TVAAASNGNFQSFSAWANPSTLHSVDWQNTLYRPGLTQNYNLAVRGGTDKVQASASAG
                     FYNQKGIVQGSYFQRVTFAGALDYTPAKWVKWSTSIKYSHQNFNVPFGSNGSNNLLSL
                     SELPPTLDSGNAKTTSIKDANGNYGFFNPVYVYVAKYSNPLYSIENNRYQNLNNYFLI
                     SSYLELTLLEGLKFKTNAGITYNGYNGYYFAPEDDRLVNQYGALAGATQNAAYSQSIN
                     NSFDWLWENTLSYDKTFGKH"
     gene            1847..5911
                     /locus_tag="VGQ51_13335"
     CDS             1847..5911
                     /locus_tag="VGQ51_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9037609.1"
                     /translation="MREFKFEISFVFFFAVFACGKAKGQSYYFNHFQVENGLSNNSVE
                     CSIQDDDGFLWFGTINGLNRFDGYAFKTFYHDPGDTGSIGSNFIRCLYSDAHGVIWVG
                     TNKGVYTFDKFREKFNLVRSLPKGNSTEILSDGRGCFWFIIDQAVYCLKPEGGKDADP
                     SGGKDADPSDGKYAEPASGKCKVYTMDGLAPVAKSIALTPDRSLWVSTANGCLKKYLP
                     ERDSFYTVSTYHLPSDPLAVSIEKIYPMNDSNFLIGTKNEGVKIFDTKTGRYRDIITS
                     NKEGSGIYVRSVIRRSGSEYWIGTETGVYIYDNTNGSVTHLQREYDNPYSISDNVVFA
                     FCKDREGGLWIGTYLGGINYLPDRFVFFEKFFPRPGTPSLSGNAVHEITKDRFGDFWI
                     GTEDGGVDKYSPVSGTFTAFKPTASKGSIAYHNIHGLLAVGDELWIGTFEHGLDVLNI
                     PTGKVIRHYNAGPGEHSLKSNFIVTIYQTREHELLIGTWGGLFRYNRESDDFSPVRGF
                     AAPIQAILEDDEGILWCCTQGDGIFYHDRKTGRYGNLRYDPNDAGSLVDNHVNGIFED
                     SRKNLWFATEGGLCRWDRAGGKFARYTTRDGLPDNLIFRILEDDKRNLWISTSKGLVC
                     FNPDSRDMRTFKRSNGLLSDQFNYNSSYKDTDGMLYFGSVKGMIGFNPAQFGKNEAVP
                     PIYITGLQINNREYTVDGRNSPLKESIAYTRMVSLPYDQSTISIDFAALSYIVPEMNE
                     YAYKLENFDKEWTYLKTNRKAYFTKLPPGRYIFKVKGSNSSGTWTGKEAQIVIEIRPP
                     YWAGSWAYVLYALGFVSLAWFIIRWYTARAAEKNRRRMELLEREKEREIYHAKIEFFT
                     NVAHEIRTPLTLIKLPLDKLVKKQSQHPEIIENLRTMERNTNRLIDLTNQLLDFRKTE
                     TDKFSLNFVKTDISELIRETFSNFQPVAEEKALQFRMELPRLTLQAFVDPEAVRKILN
                     NLVNNAIKYAGSKVVVQLLPFNSEANVFSIEVRNDGLLIPYEQKEKIFQPFYRGKGAE
                     KQPGTGIGLPLARSLAELHKGVLDLKQPEGDLNVFLLVLPIHQDQEFVLQSDEATNVT
                     SEEDVVKDRSGPVILFVEDNKEILDFIARELQGEYIVKKALNGKQALEILKEESVQLI
                     ISDIMMPVMDGIELCKTVKTDLEFSHIPIVLLTARNTLHSKIEGLEVGADAYIEKPFV
                     LEHLRAQLSNLLTNRAKIKEYFASSPLVHIKSMGCSKADRQFLEQLDTIIHENLGNTD
                     IDVEHLARRMNMSRATFHRKVKALSNLTPHDLINISRLQKAARLLADGDYKVYEVADM
                     VGYSLASNFARDFHKQFGMTPTEYVNNRKNLSRI"
     gene            5908..7056
                     /locus_tag="VGQ51_13340"
     CDS             5908..7056
                     /locus_tag="VGQ51_13340"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEV9037610.1"
                     /translation="MKEDRRNFIRKSAVLAAAFSGKAALPQGLAGGRSVARSRSAADR
                     SGAEYVRDAGMQMSEAYFDGFNQHKIALCRQMEVLGAVGGINPKMVGLNDVKPWDYAA
                     VQAVKNAWDNEGLKMTVVEGPPALGEKTKLGLEGRDEEISNFITLMQNLRRVGIEVIC
                     YNWMPVIGWYRTDNAKKGRGGALVTAFDYEAVKGLPPTEYGVVTQEQLWKNLEYFLKA
                     VVPEAEKAGIKLAMHPDDPPIDVGIRGIARIMTSAENFSRMLDIYPSPSNGITMCQGN
                     FSLMGVDIPTVVREFGRRKAIHFVHFRNVRGGKFKFEETFHDEGQIDMYEAMKAYYEI
                     GFRGPIRPDHVPTMAEDSNTFAGYSTIGTLYANGYIRGLMEAVAKEKK"
     gene            7061..7993
                     /locus_tag="VGQ51_13345"
     CDS             7061..7993
                     /locus_tag="VGQ51_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEV9037611.1"
                     /translation="MHKSLFLVLIFLAGAGSTRAQRVGSSPETIIALTSRWKGERFPD
                     GRPKVPEELLERLKPLSLEDVWGVLRNKGYQNQFEGNWQIVNPDSGMTGRVVTAQYMP
                     LRPDLDSLVKAEGKAEGRGQHGGTNSWPIDILVDGDVYVADSYEKMADGTLIGDNLGN
                     SIYAHSHRGFIFYGSVRDMAGLSEIRGFNGWMKGSDPSYIQQMMLTTINAPIRIGRAT
                     VLPGDVVLANKWGVVFIPSYLVQEVVITSEVTELRDEFGHQRLREKKYLTGQIDSQWT
                     EEIRKDFLDWLNHYPGKLPMSKKELDDYLKERNY"
     gene            7996..>8443
                     /locus_tag="VGQ51_13350"
     CDS             7996..>8443
                     /locus_tag="VGQ51_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV9037612.1"
                     /translation="MNKTRRVFFRKAALSGLAMGGLFGMSFEDRVAETTGDVRRASAP
                     SDLKITDMRYAVVQNVGRTAIIRIDTNQGIYGLGEVRDGADERYALMLKSRILGQNPC
                     NVEMIFKMIRQFGGPSRQAGGVCAVEMALWDLCGKAYNVAAWQLLGG"
BASE COUNT         2043 a         1914 c         2553 g         1933 t
ORIGIN      
        1 atgtttgccg aaggtcttgt cataagaaag ggtgttctcc catagccagt caaaactgtt
       61 gttgatgctt tgactgtagg cggcgttctg tgtcgcgcct gccaatgctc catattgatt
      121 gaccagccgg tcgtcctccg gcgcgaaata gtagccgtta taaccattat aagtgatacc
      181 ggcattggtc ttgaatttca gcccttcgag aagcgtgagt tccaggtaac tgctgatgag
      241 gaagtaattg ttcaggttct gataccggtt gttctcgatg ctataaaggg gattggaata
      301 tttagccaca tagacatata cgggattgaa gaaaccgtag ttgccattag catccttgat
      361 cgatgtggtc ttcgcgttgc cgctgtccaa tgtcggcggc aattcgctta gggaaagcag
      421 attgttactg ccattggaac cgaagggcac attgaagttc tggtgtgaat atttaatgct
      481 ggtggaccat ttcacccatt tggccggcgt gtagtccaat gccccggcaa aggtgacgcg
      541 ctgaaaatag gagccctgaa cgataccttt ttggttgtag aagccggcgg aggcagaagc
      601 ctgtaccttg tcggtgcctc ccctgacggc gaggttatag ttttgtgtca atcctggacg
      661 gtaaagcgta ttctgccagt cgacgctgtg cagggtggag gggttggccc aggcggagaa
      721 ggactggaaa ttgccgttgg aagcagcggc gaccgtattg gccagtgtac cgaactgttc
      781 cgcattcagc accttatatt ctttgggttt gccctggaac gcctcgtaca tatcgaagga
      841 tatctgtgcg ccgctacgtt tgcccctttt ggtagtgatg atcacgacgc cgttggccgc
      901 gcgcatacca taaatggcgg tagcggaggc atccttgagg atgtcgatcg tggcgatatc
      961 gttggggttg atattgttga tcccgcctgt ttcgagcgga tagccgtcga ccacatacag
     1021 cggagagtta ccgttgttgg cgaggctgcc gatgccgcgg atcaggacgt tgatattacc
     1081 gccgggagag gcgtcgttct gggtgatctg aacgcccgcg gcgcgtccct gcatcgcctg
     1141 ttcgagcgtg ggagtaggca ccttttcgat ctgctcggcg cttacgctgc tgatggcgcc
     1201 tgtcaggtcc ttgcgttttt gcgtgccata gccgatgacg accacctgtt gaatgctttg
     1261 attttcggga gccaggcgga cgttgaagct ggattgggtc ccgatgcgga tctcctgcgg
     1321 tgtgaagccg acataactga cgatcagcgt agcattggcg gggaccgaaa gtgtgaatgt
     1381 gccgtcctgt ccggtggtcg tgccgatctt gctccctttc acggaaacgg tcgcgccgac
     1441 gatcgcgctg tcgctctcgt ttccgacgac tttgcctgtg atctgccgtg actgcccgaa
     1501 tacggcgccg ctgaagcaga ccagcgacag gatgaccagc agccttctat aagcatttgg
     1561 gtaaagcagc gagttttggt tcattttttt ttgtttaaag ttggtaattg ccgcgtagcg
     1621 aagaaatctg cattttatca taagcaatcg aattgaatag gtagtatgag cacgtcaggc
     1681 aggcccgtgt ttggttgtct tgcaaagcta ctgttaagcc aatcaaagaa cggtattttc
     1741 ggccggcttt tcctgatcaa tatttatcaa aatcttttca aattttatca attcgagaat
     1801 atcgtgcaat ggacagccga ctttttaggc taatttgtac ttcgtaatga gagagttcaa
     1861 gtttgagata tcgttcgttt tcttttttgc ggtttttgcg tgcggcaaag cgaagggtca
     1921 gtcttattat ttcaatcatt ttcaggtgga gaacgggttg tcgaataact cggtcgagtg
     1981 cagtatacag gatgacgatg gatttctgtg gttcgggacg atcaatgggt tgaaccgttt
     2041 tgacggatat gctttcaaga ctttttacca tgatcccggg gacacgggca gcatcgggag
     2101 caattttatc cgatgcctgt atagtgacgc acatggcgtt atctgggtgg gcaccaacaa
     2161 gggcgtttat accttcgaca aattcagaga gaagttcaat cttgtgcgga gcctgccgaa
     2221 aggcaactcc acggagatac tcagtgacgg aagaggatgt ttttggttca ttatcgatca
     2281 ggctgtttat tgtctgaagc ccgagggcgg gaaggatgcg gacccatctg gcgggaagga
     2341 tgcggaccca tccgacggga agtatgcgga gccggcgagc ggtaagtgta aagtctacac
     2401 aatggatgga ctggcgccgg tagcgaaatc gatcgcgctc acgccggatc gttcgctttg
     2461 ggtctctacc gcgaatggat gtctgaagaa atatcttccg gagcgcgatt ctttttatac
     2521 ggtaagtacc tatcatctgc cgtccgatcc gctggccgta tcgatcgaga agatctaccc
     2581 gatgaatgac agcaacttcc tcatcgggac gaagaacgag ggggtaaaga tcttcgatac
     2641 gaagacgggc aggtacaggg acatcatcac ttccaataag gaagggtcgg gcatctatgt
     2701 gcgatcggtg atccggcgga gcgggagcga gtactggatc ggcacggaga cgggggtcta
     2761 tatctacgac aataccaacg ggagcgtaac gcatttgcag cgggaatacg ataaccccta
     2821 ttcgatatcg gacaacgtcg tctttgcgtt ctgcaaggac agggaagggg gattgtggat
     2881 cgggacttac ctcggaggga tcaattatct gcctgaccgt tttgtctttt tcgagaaatt
     2941 ctttccgcgc cccggaacac cgtcgctgag cgggaatgcg gtgcatgaga tcacgaagga
     3001 ccggttcggg gatttctgga tagggacgga ggatggaggc gtcgataaat actcgccggt
     3061 ttccgggacc tttacggctt tcaaaccgac agcttcgaaa ggcagtatcg cgtatcataa
     3121 catccatgga ctcctggcgg tgggcgacga gctctggatc ggcaccttcg aacatgggct
     3181 ggacgtgctg aatattccga caggtaaggt gatccggcat tataatgcgg gcccgggcga
     3241 gcatagcctg aaaagcaatt ttatcgtaac gatctatcag acgcgggaac atgagttgct
     3301 gatcggaacg tggggaggtc tgttccggta taaccgtgaa agcgatgatt tttcgccagt
     3361 gcggggattc gcggcgccca tccaggctat tctggaggat gacgaaggca ttctctggtg
     3421 ttgtacacag ggcgacggta tcttctatca tgatcggaag acgggccggt acggtaatct
     3481 ccggtatgat ccgaacgatg cgggcagcct ggtcgacaac catgtcaacg gaattttcga
     3541 ggacagcaga aaaaacctgt ggttcgcgac tgagggaggt ctttgcagat gggatcgggc
     3601 aggaggcaag ttcgcgcgct atactacccg ggacggatta ccggacaatc tgatcttccg
     3661 tatactcgaa gatgacaaaa ggaatctttg gatcagtacc tccaaggggc ttgtgtgttt
     3721 caaccccgat agccgggata tgcgaacctt taagcgatcg aacggtctgc taagcgatca
     3781 gttcaattac aactcttcct ataaggatac ggatgggatg ctttatttcg gcagcgtcaa
     3841 gggaatgatc ggcttcaacc ccgcgcagtt cgggaagaat gaggcggtac cgcctattta
     3901 tatcacgggc ctccagatca acaaccggga atatacggtg gacgggcgga attccccgct
     3961 gaaggaatcc attgcttaca cccggatggt ctcgctgccg tatgaccaat cgacgatcag
     4021 catcgatttt gcagcattga gctatatcgt cccggaaatg aatgaatatg cctacaaact
     4081 ggagaatttc gacaaggagt ggacctatct caagacgaac agaaaggcgt attttacgaa
     4141 actgccgccc ggaagatata tctttaaagt aaagggatcc aacagcagcg gaacctggac
     4201 cgggaaagag gcgcagatcg tgatcgagat acggccaccc tattgggcgg gcagctgggc
     4261 ttatgtgctg tatgcgctgg ggttcgtgag cctcgcctgg ttcatcatcc ggtggtacac
     4321 ggcgcgggcg gcagagaaga acaggcgaag aatggaattg ctcgaaagag aaaaagaaag
     4381 agagatctat catgccaaga tcgaattctt tacgaatgtc gcacatgaga tacgaacgcc
     4441 attgaccctg ataaaactgc ctcttgacaa actggtgaag aaacagagtc agcatcccga
     4501 aataatcgaa aaccttcgca caatggaaag aaacacgaac cgactgatcg atctgacgaa
     4561 tcagctgctt gattttcgca agacggagac cgataagttc agtctgaatt tcgttaaaac
     4621 ggatatttcc gaactgatca gggagacctt ttccaatttc caacccgtag ccgaggaaaa
     4681 ggcccttcaa ttccggatgg agctgccccg gctcacgcta caggcattcg tggaccccga
     4741 agctgtccgt aaaatactga ataacctggt caacaatgcg atcaaatatg ccgggagcaa
     4801 ggtcgtcgtg cagttgctgc ccttcaacag cgaggccaac gtattcagca tcgaggtccg
     4861 gaatgacgga ttgctgatcc cgtatgagca gaaggagaag atcttccagc ccttctatcg
     4921 cgggaagggg gcagagaaac agccgggaac gggcatcggg ttgccactgg cgcggtcgct
     4981 ggccgagctg cataaaggcg tgctcgatct gaagcagccg gaaggtgacc tgaacgtctt
     5041 tctgctggtg ctacccattc accaggacca ggaattcgta ttgcaaagcg acgaagcgac
     5101 gaatgtgacg tcggaggaag atgtcgtgaa ggaccggtcg ggacccgtca tccttttcgt
     5161 ggaagacaac aaggagatcc tggattttat cgcgcgcgaa ttgcagggcg agtatatcgt
     5221 gaaaaaggcg ttgaacggga aacaggcatt ggaaattctg aaagaagaga gtgtccagct
     5281 gatcatctcc gatatcatga tgcccgtcat ggatgggatc gaactctgca aaacggttaa
     5341 aacggacctg gagttcagtc atatccccat cgtgctgctg accgccagga atacgctcca
     5401 ttcgaagata gaggggctgg aagtcggcgc cgacgcttat atcgagaagc cttttgttct
     5461 ggaacatctc cgcgcgcaat tgtcgaacct gctgaccaac agggctaaga tcaaggaata
     5521 ttttgcaagc agccccctgg tgcatatcaa aagcatggga tgctcgaaag ccgacaggca
     5581 attccttgaa cagctggata cgatcattca cgagaacctt gggaataccg atatcgacgt
     5641 ggagcacctg gccaggcgca tgaacatgag cagggcgacc tttcatcgta aggtaaaggc
     5701 gttgtcgaac ctgaccccgc acgatctgat caatatctcg cggttgcaga aagccgcacg
     5761 tctgctggcg gacggagatt acaaggtgta tgaggtggcc gatatggtcg ggtattcgct
     5821 cgcatccaat tttgcgaggg atttccacaa gcagttcggc atgaccccga ccgagtatgt
     5881 caacaacaga aaaaatcttt cccgtatatg aaagaggata gaaggaattt tatccggaag
     5941 agcgctgttc tggcagcagc attttccggg aaggcggcgc tgccgcaagg gcttgccggt
     6001 ggccgttcgg tggccaggtc gcgttcagcg gcggaccgga gtggcgcaga gtatgtccgg
     6061 gatgcaggaa tgcaaatgtc ggaagcctac ttcgacggct tcaaccagca taagatagcc
     6121 ttatgccggc agatggaggt cttgggcgcg gtgggcggta tcaatccgaa gatggtggga
     6181 cttaatgacg tgaagccgtg ggattatgcc gccgtgcagg cggtaaagaa tgcctgggac
     6241 aacgagggtc tgaaaatgac ggtcgtcgaa ggaccgccgg cgttgggcga gaagacgaag
     6301 cttggtctgg agggaaggga tgaagaaata tcgaatttta taacattgat gcagaatctg
     6361 cgtcgggtgg gcatcgaggt tatttgttac aactggatgc ccgtgattgg gtggtaccgg
     6421 accgataatg ccaaaaaggg gcgcggcggc gcattggtaa cggctttcga ttatgaagcc
     6481 gtgaaggggc tcccgcccac cgaatatggc gtagtaacgc aggagcaatt gtggaagaac
     6541 ctggagtatt ttctgaaagc ggtggtgccg gaggcagaga aggcgggcat caaactggcg
     6601 atgcatccgg atgatccgcc aatcgatgtc gggatcagag gtattgccag gatcatgacg
     6661 agcgcggaga atttttcgcg tatgctcgat atttatccca gcccaagcaa tgggatcacg
     6721 atgtgccagg ggaatttttc gctgatggga gtagatatcc ctacggttgt acgggagttc
     6781 ggtcggcgaa aggcgatcca tttcgttcat ttccgcaatg tccgcggtgg gaagttcaaa
     6841 ttcgaagaga cgtttcatga cgaaggacag atcgatatgt acgaagctat gaaggcgtac
     6901 tatgaaattg ggttcagggg accgatacgg ccggatcatg taccgacaat ggcggaggac
     6961 agcaacacgt tcgcgggtta ctcgacgatc gggacgttgt atgcgaatgg gtatatccgg
     7021 gggttgatgg aggcggttgc aaaagagaaa aaataagagt atgcataaaa gcctgttttt
     7081 agttttaatt tttctcgcgg gcgctggttc tacccgggcg cagcgggtgg gttcgtcacc
     7141 ggagaccatc atcgcgctga cttcgagatg gaagggcgaa cgcttccccg acgggagacc
     7201 gaaggtgccg gaggagttgc tggaaagact taaaccgttg tcgctggagg atgtgtgggg
     7261 cgtattgcgc aataagggct atcagaacca gttcgaaggc aactggcaga tagtcaatcc
     7321 ggacagcggg atgacgggca gggtggtgac ggcgcagtac atgccgttga gaccggacct
     7381 ggatagcctt gtcaaagcag agggaaaggc ggaaggccgc gggcagcacg gaggtaccaa
     7441 ttcctggccg atcgatattc tggtggacgg tgacgtgtat gtcgccgaca gctatgaaaa
     7501 aatggccgac gggacgctga tcggcgataa cctgggaaat tccatctatg cacattcgca
     7561 tcgcgggttt attttttacg gttcggttcg cgatatggcg ggattgtcgg agatcagggg
     7621 tttcaatggc tggatgaagg gtagcgatcc atcctatatc cagcagatga tgctgaccac
     7681 gatcaatgcg ccgatccgga tcggcagggc gacagtgtta cccggggatg tcgtgctggc
     7741 caacaaatgg ggtgtcgtct ttatcccatc ctacctggtg caggaagtgg tgatcacctc
     7801 ggaggtcacc gagctacggg atgaattcgg tcatcagcgt ctgcgggaaa agaaatacct
     7861 gacgggtcag atcgacagcc aatggaccga ggagataaga aaggactttt tggattggct
     7921 gaatcattac ccgggcaagc tgcctatgag caaaaaagaa ctggatgatt atctcaaaga
     7981 aagaaattac tgattatgaa taagactcgt cgggtttttt tcaggaaggc cgcgcttagt
     8041 ggcctggcta tgggggggtt gttcgggatg tcgtttgaag acagggttgc cgagactacc
     8101 ggcgacgtac gccgggcgtc ggctccttcg gacctaaaga taacggacat gcgatacgcc
     8161 gtcgtgcaga atgtgggccg aacggcgatc atccgtatcg acactaacca ggggatctat
     8221 gggctcgggg aggtcaggga cggcgcggat gagcgatatg cgctgatgct gaagagccgc
     8281 atcctgggcc agaacccatg taacgtagag atgatcttta aaatgatcag gcagttcggc
     8341 ggtccgagcc ggcaggcagg cggcgtatgt gcggtagaga tggccctgtg ggacctgtgc
     8401 ggcaaagcgt acaatgtcgc ggcctggcaa ctgctgggcg gga
//
LOCUS       DASYSV010000434         3764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2135308, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000434 DASYSV010000000
VERSION     DASYSV010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2135308"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VGQ51_13355"
     CDS             <1..410
                     /locus_tag="VGQ51_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9037613.1"
                     /translation="TYAYQLQSYNLMNFLEFVSDHYATAYVDHYFNGFFFNKIPLLKK
                     LKWREVIEAKILYGGLRAENNPAQNPEQIKFPFTNGQLETYSLTNVPYVEAGFGIANI
                     FKLIRVDFIKRFTYLDHPNIPTWGIRARAKFDF"
     gene            521..1903
                     /locus_tag="VGQ51_13360"
     CDS             521..1903
                     /locus_tag="VGQ51_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4833 domain-containing protein"
                     /protein_id="HEV9037614.1"
                     /translation="MSTRKKLQSGIYRVINPLVRLLIRMGLTPNMVTTIGLLLNIGVA
                     VIFVLGAEEGRRGDLSYVGWAGALILFAGLFDMLDGQVARLGNMSSPFGALYDSVLDR
                     YSELILFFGICYYLVAHHYFVSSLFAFAGLIGSMMVSYTRARAEGLGIECKDGLMQRP
                     ERVIILGVSALACGIASRWMGGDYKISWPDATFPVFETMSIFTIPLTVMAFLANMTAI
                     GRLRDARRTLEARSANGAAVRSDASGKPDGTAKSDAATKPAAKAKTGTMIILLLGGLS
                     AMPGTAQPAGRTATPATMHPRSVVIADPVDTFPIPPANSNSLFYLQRTPNTNTIICEL
                     NETAGILDKDEPVRVLWIRYTEQRQREELTWIQRHFAYGLKERDMGNDIYELRFVSYK
                     QIPMYLKRSSVDNKRHVYVSIDGRQAILQRVYIRIDPGGSFWAPNVRYLELKGVDTQN
                     GKELIQRIKV"
     gene            1909..2841
                     /locus_tag="VGQ51_13365"
     CDS             1909..2841
                     /locus_tag="VGQ51_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV9037615.1"
                     /translation="MDTGTALTTGTPLSRRNVLIVSGLSLFYLLGSAWLIGYNNDQLF
                     LVLLFNSLFYLSGPTRRFILGFSVFIVFWVLFDSMKAFPNYRYNLVHIGDVYALEKGL
                     FGIHGLTPNEYWLAHQRVSLDVAAGFFYLCWMPLPLAFAGYLFYKNKTMFLRFALTFL
                     LVNLIGFVIYYVFPAAPPWYVEQYGFVFHPHTPGNTAGLGRWDSYFHVSVFAGLYAKS
                     SNVFAAMPSLHAAYPLTVLYYGIRSRLGWWNALFALIMVGIWFAAVYASHHYVLDVLA
                     GIGCGIAGILLFNSWYSRDGWFARLVGKWARAIS"
     gene            complement(2896..3495)
                     /locus_tag="VGQ51_13370"
     CDS             complement(2896..3495)
                     /locus_tag="VGQ51_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV9037616.1"
                     /translation="MAETLGRDETVVKEIIDTARALFKKTGFKKTTMGDIARGLGKAK
                     SSLYYYYPSKEDIFEAVVFTEMDELLEQINQSIRKASSSKEKLIIYCRCRLEKLSQLC
                     NLSETLKTEITELQCLIGHLKNRFDTSHVELVKEILAEGVRNNEFKKINAGNIELVAY
                     LMVSSFRGLAVPLMVSGDQSPRIDVQIDSIVDIMVEGIS"
BASE COUNT          779 a          941 c         1046 g          998 t
ORIGIN      
        1 agacctacgc ctatcagctg caatcgtaca acctgatgaa cttcctggaa ttcgtcagcg
       61 accactatgc gacggcctat gtcgaccatt atttcaatgg atttttcttc aataagatac
      121 cgctgttgaa gaaattgaaa tggagagagg tcatcgaagc gaagatcctg tatggcgggt
      181 tgagggccga gaataacccg gcgcagaatc ccgagcagat caaattcccg ttcacaaacg
      241 ggcagctcga gacgtattcg ctgaccaatg tgccctatgt cgaggcgggt tttggtatcg
      301 ccaatatttt caagctgata cgggttgatt ttatcaagag gttcacctat ctggatcatc
      361 cgaatatccc tacgtggggg atacgggcga gggcgaaatt tgatttttag gggatgcggc
      421 gcgattcggg gcgtccactt tcagggggcg atgcactgcg gtccgcgtcg gatttttggt
      481 tatctttaca gattgtcatc agaaagagag tcatggctaa atgagcacgc gaaaaaaatt
      541 acaatcgggg atttatcggg taataaaccc gctggtccgg ctgttgatcc ggatgggatt
      601 gacgccgaat atggtcacga cgatcgggct gttgttgaat atcggggtgg ccgtcatctt
      661 cgtcctgggt gccgaggaag gtcgcagggg cgatctgtcc tatgtcggct gggcgggagc
      721 cctgatcctt tttgccggat tgttcgacat gctggatgga caggtggcac gattgggcaa
      781 catgagctcg cctttcggtg cattgtatga ttcggtttta gacaggtata gcgagctgat
      841 cctttttttc ggcatttgtt attatctcgt ggcgcatcac tatttcgtga gcagcctttt
      901 tgcttttgcc gggcttatcg ggagcatgat ggtcagctat acgcgggcgc gcgccgaagg
      961 gctgggtatc gaatgtaagg acgggttaat gcagcgtccg gaacgggtca tcatcctggg
     1021 tgtatcggcg ctggcttgcg gcatcgcttc ccggtggatg gggggcgact ataagatctc
     1081 ctggcccgat gcgaccttcc cggtcttcga gaccatgtcc atctttacaa tccctttaac
     1141 ggtgatggcc tttttggcga atatgacggc tatcggaagg ctgcgcgacg cgagaaggac
     1201 gctggaggca aggtcggcga atggcgcggc cgtcagatcg gacgcctcgg gcaaacctga
     1261 cggaaccgct aaatctgacg cggccaccaa acctgccgca aaggcgaaga cgggcaccat
     1321 gatcatcctt ttactgggtg ggttgtccgc tatgcccggc accgcccagc ctgccggaag
     1381 gaccgcgacg ccggccacga tgcatccgcg ttccgtcgtc atcgccgacc cggtggatac
     1441 ctttccgatc ccccctgcca actccaattc attgttctat ctgcaacgca cacccaatac
     1501 caatacaatt atttgtgagc tcaatgagac ggccggcatc ctggataaag atgagccggt
     1561 gcgggtatta tggatccgtt atacggaaca acggcaacgc gaggagctga cctggatcca
     1621 gcgtcatttt gcttatggcc ttaaggagcg cgatatggga aatgatatat acgagctgag
     1681 gttcgtctcg tataagcaga taccgatgta cctgaagaga agctccgtcg acaataaacg
     1741 acacgtctat gtctccatcg atggaagaca ggccattctt caacgggtgt atatccgcat
     1801 cgatccgggc ggcagcttct gggcgccgaa tgtccggtat ctcgagctga aaggcgtcga
     1861 cacgcaaaat ggcaaagaat tgatccaacg tatcaaagtt tagcgcacat ggataccggt
     1921 accgcgttaa ccacaggcac gccgcttagc cggcgcaatg tgctgatcgt ctccgggctc
     1981 tcgctgttct atttgctggg cagcgcgtgg ctcatcggct ataataacga tcagctgttc
     2041 ctggtcctgt tattcaacag tcttttttat ctctcgggtc cgacgaggcg ttttattctc
     2101 gggttcagcg tctttatcgt gttttgggtg ctcttcgatt ccatgaaggc ttttcccaat
     2161 taccggtata acctcgtgca tatcggggat gtttatgcgc tggagaaggg gcttttcggc
     2221 atccatggac tgacgccgaa tgagtactgg ctcgcgcatc agcgtgtctc cctcgatgtc
     2281 gcggccggtt ttttctatct ctgctggatg ccgctgccgc tggctttcgc cgggtacctg
     2341 ttctataaga ataagaccat gtttttgagg tttgcgctga cctttttgct cgtgaatctc
     2401 atcgggtttg tcatttatta tgttttcccg gccgcgccac cctggtatgt ggagcaatat
     2461 gggttcgtct tccacccgca tacgcccggg aatacggcag gtctggggcg ttgggacagc
     2521 tatttccatg tttccgtttt tgcggggcta tatgccaaaa gcagcaacgt gttcgcggcc
     2581 atgccttctc ttcatgcggc ctatccgctt accgtattgt attatgggat ccggagtcgt
     2641 ctcggctggt ggaatgcgct gtttgcgttg atcatggtgg gtatctggtt tgcggcggtg
     2701 tatgccagcc atcattatgt gctggatgtg ctggccggta tcggctgcgg tattgccggc
     2761 atcctgttgt tcaattcctg gtattcgagg gacgggtggt ttgcgcggct ggttgggaaa
     2821 tgggcgcggg cgatcagcta gacgcgcttg gaccgccgga tgtcttcagc gaggcgaacc
     2881 gaccgccgga gccgatcaac tgatgccttc caccatgata tctacgatag agtcgatctg
     2941 gacgtcgatg cgaggagatt gatccccgct taccatcagc ggcacagcca gtccccgaaa
     3001 actactgacc atcaggtaag caaccagttc aatattgccg gcattgatct ttttgaattc
     3061 attgttgcga acgccttcag ccagaatttc tttaaccagc tccacatgcg aagtgtcgaa
     3121 cctgtttttc aggtgtccta taaggcattg cagctcagtg atttccgttt tgagggtctc
     3181 gctgaggttg cacagctggc tcaacttttc cagtcggcac cggcaataga tgatgagttt
     3241 ttctttcgat gaggacgcct tgcggataga ttggtttatc tgttccagca gctcatccat
     3301 ttcggtaaaa accacggctt cgaaaatatc ctctttgctg gggtagtaat agtaaagcga
     3361 gcttttggcc ttaccgaggc cgcgggcgat gtctcccatc gtcgtttttt tgaatcccgt
     3421 ctttttgaaa agggccctcg ccgtgtcgat aatctcctta accaccgtct cgtcccgccc
     3481 aagcgtttct gccatagtgc atgtagcttt atgcggtcaa atttaggcta tttctttccc
     3541 ggaagcggtt aaacgatcgt gaatcttgta tctctgccgg actatttcta catttacgtg
     3601 actgcttatg accactcaat tgaattgtat ccttctcatc gaggatgatg aacccacaaa
     3661 cttcctgaac aggatgaccc tggagcaggc gcgttgcgca cgtcatatcc gtattgcacg
     3721 tagcggaaaa gaggccctga actatttggg aaaatgcggg ggag
//
LOCUS       DASYSV010000435         5280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_213431, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000435 DASYSV010000000
VERSION     DASYSV010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213431"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VGQ51_13375"
     CDS             complement(<1..770)
                     /locus_tag="VGQ51_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEV9037617.1"
                     /translation="MRKTILSCCFIAIFSLGALAQSATIKIDVDRVIADIDPKIYGVF
                     MEPIHFNGSRMGLPDTVNFNTLYGTLYDPKSPLADENGFRKDYIDAMRELRITNMRWP
                     GGNFLMGYDWKDGIGPKEQRPTRINLAWGGPETNHVGTDEWMALNKAIGSENIVCVNL
                     GLGSIWDAAYWVEYCNYKKGTYYSDLRAKNGHPEPYHVKIWDLGNEVDGLPWELGHKN
                     AEDYVETAREAAKAMRSVDPTIKFVASGSSYYEPTNKW"
     gene            complement(784..3318)
                     /locus_tag="VGQ51_13380"
     CDS             complement(784..3318)
                     /locus_tag="VGQ51_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEV9037618.1"
                     /translation="MKVTIATVTILVSLACGVRAIAQNADPGPLLINDSGYFEKPGVN
                     VLVFSSQYNGMFFDEKTAGLEIIHHGVRTATGGAIRLQNTPEQWDLIPVMVSRQVKKD
                     AGTIETVLTYKEYNFTSRIRVTAKDKGVEIDVYLDEPLPEKLVGNAGFNLEFLPAAYF
                     EKTYFADGHPGNFPLYPSGNTKMESVDKKIPQFAGHTTFDDRGRHEFIIPSPLATGRT
                     LVLAPEDPERKVLIRSTGTDLMLFDGRNLGQNGWFIVRSLLPAGKTGKVLSWYLEPNA
                     IPGWIRKPVIGFSQAGYTPGQNKTAVIELDKSDVPLQEATLYQVTEDGRQVEKLRGPL
                     HSWGRYLRYNYLTFDFSAVKDPGVYFLQYGSQRTNSFRISPDIYADIWHPTLDVWFPV
                     QMDHMEVNEAYRVWHGASFLDDALQAPVNHQHFDGYRMGPSTQTKYKPLERIPGMAVG
                     GWYDAGDFDIQTGSHCDAVLAFVDAWEKFKPTRDETFVDYPTRYVDIHRPDGKPDLLQ
                     QIRHGTLNMVAQVENIGHPVRGIVVPNLHQYHHLGDGSMETDNLPYNPQLKPYETDGR
                     SSGTMDDRWAFTERSVFLDYYTAAALAAASRALKGFDDTLAARSLAAARKLWNEDDSS
                     GRQDNSQIPAVFRRSLKATAALQLYITTREEPFAEAFRAGIWPMLDRGVQFGMLTALQ
                     AGPYMDQAYRDRLREYVARYKVLCDDYSRQNPYGVPMATGGWGGNSGIVNWAITCYYA
                     HRYYPDLMPVDYIYKGLNYLFGCHPYSNISFVSAVGTRSKKITYGNNRADFSYIAGGV
                     VPGILILKPDFPENKEDWPFFWGENEVTVGICANYILLATAVNTLQ"
     gene            complement(3439..>5280)
                     /locus_tag="VGQ51_13385"
     CDS             complement(3439..>5280)
                     /locus_tag="VGQ51_13385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator transcription factor"
                     /protein_id="HEV9037619.1"
                     /translation="VNTARYTNLDPGEYLFRVKASNNDGLWNTEETTIKIYVRPPFWR
                     TAYAYIFYVLAIGGLLAYSRHRGLSRVRKKFLLERERAEAARTRELDRMKLKFLTNLS
                     HEFRTPISLIMAPVSELLSRHQEDNSKDKLNMIRRNARRLLNLVNQLLDFRKMEEQEL
                     RLVLTEGDLVAFVDDVVHSFSDISGRKHIRLNFTFNTPRLHLLFDHDKIERILFNLLS
                     NAFKFTLEEGTISVAMNASDNPDPDGRLWVSIGVIDSGIGIPADKLDRIFDRFFQHAS
                     GNAVLNQGTGIGLSIAKEFAEMHGGTIGVESEPGRGTTFTLRLPLQVVNGAGSPAILE
                     GTGERWETPAEDSAVDSSEKPIVLLVEDNDDFRFYLKDNLKGNYRIIEATNGKEGWHK
                     ALALHPQLIVSDISMPHMDGIVLSRKLKADKRTCHIPIILLTALTGEVQQLQGLETGA
                     NDYITKPFNFEVLHAKIRSLLALRRDLATTYGRQIKMMQPEIKIETEDERLLQAVVRC
                     LEENIVSSQLSVDFLSRQVGMSRSSLYSKLLEITGETPVEYIRSFKLGKAATLLEKSD
                     LTISEIAYQTGFSKPGYFARAFKAKFDMLPSDYIAQKRKTGLNKPIN"
BASE COUNT         1155 a         1440 c         1365 g         1320 t
ORIGIN      
        1 aaccatttat tggttggttc atagtaggat gagcccgatg cgacaaattt gatggttgga
       61 tccaccgacc tcatcgcctt tgcggcctct ctcgccgtct ccacataatc ttcagcattc
      121 ttatgaccga gttcccaggg aagcccgtcc acttcgttac ccagatccca tatcttcacg
      181 tgataaggtt cggggtggcc gttctttgca cgcaaatcgg aatagtaagt cccttttttg
      241 taattacaat attccaccca atacgctgcg tcccatatgg aaccaagacc gaggttcacg
      301 cagacgatat tttcgctgcc aatggcttta tttaaagcca tccactcatc ggtgccaaca
      361 tggttggtct ccggcccgcc ccacgccagg ttgatgcgcg ttggacgttg ttctttcggg
      421 ccaataccat ctttccagtc atagcccatt agaaagttgc cgccgggcca ccgcatattg
      481 gtaattctta attcgcgcat ggcgtctata taatcttttc ggaaaccatt ctcatctgca
      541 agcggagatt tcggatcata gagggtgcca tacagcgtgt tgaagttaac cgtatccggc
      601 aaccccatcc ggctcccatt gaaatggatc ggctccatga atacgccata gatcttcgga
      661 tctatatccg caataacgcg atcgacgtca attttgatgg tggcggactg cgcgagcgcg
      721 ccgagggaaa atattgcgat aaaacagcag gacaatatcg tttttctcat agttgaaaga
      781 attttattgg agggtattca cggccgttgc caggaggata tagttggcgc agatgcccac
      841 cgtcacctcg ttctctcccc agaagaacgg ccagtcctct ttattctcgg ggaagtccgg
      901 tttcaggatc aggataccgg ggacgacgcc gcccgcgata tagctgaaat ctgcgcggtt
      961 gttaccgtac gttatcttct tcgagcgggt gcccaccgcc gaaacgaagg atatattgga
     1021 gtaaggatga cacccgaaga ggtagttgag ccccttgtag atatagtcca ccggcatcag
     1081 gtcggggtaa taccggtgcg cataatagca ggtgatcgcc cagttgacaa tgccgctgtt
     1141 accgccccag ccgccggttg ccatgggaac gccatagggg ttttgccggg aatagtcgtc
     1201 acacagcacc ttgtaccggg cgacatattc ccgcagccgg tcacgatagg cctggtccat
     1261 atacggaccc gcctgaaggg cggtgagcat tccgaattgc acgccgcgat ccagcatcgg
     1321 ccaaatgcct gcccggaatg cttcagcaaa cggctcctcc ctggtcgtga tatacagctg
     1381 aagcgcagca gtagccttca ggctccttct gaaaacggcc gggatctgcg agttgtcctg
     1441 ccggcccgaa ctatcgtcct cgttccaaag ttttcgggcg gctgccaggc tccgtgccgc
     1501 aagcgtatcg tcgaaacctt tcaatgcgcg gctggcggcg gccaatgccg cggcggtata
     1561 ataatccagg aagaccgacc tttcggtaaa ggcccaccgg tcatccattg ttccactgga
     1621 tctgccgtcc gtctcatagg gcttcagctg cggattgtaa ggcaggttgt ctgtttccat
     1681 ggacccgtcg cccaggtgat ggtactggtg taggttgggg acgacgatcc cgcgcacggg
     1741 atgaccgata ttctccacct gcgccaccat attcagtgtg ccatgcctga tctgttgcaa
     1801 tagatcgggc ttgccgtccg gccggtgaat atcgacgtat cttgtcggat aatcgacgaa
     1861 cgtctcgtcg cgggtcggtt tgaatttctc ccacgcgtcg acgaaagcca gcaccgcgtc
     1921 gcaatgtgaa ccggtttgga tatcgaaatc gccggcgtcg taccagccgc caacggccat
     1981 gccggggatc cgttcgagtg gcttgtattt tgtttgcgtg gacggcccca tacgataacc
     2041 gtcaaaatgc tgatggttta ccggcgcctg cagagcgtcg tccaggaagg atgcgccatg
     2101 ccacacgcga taggcttcat tcacctccat atgatccatt tgtaccggga accacacatc
     2161 caacgtcgga tgccagatat ctgcatagat gtcgggactt atccgaaagc tgttcgtacg
     2221 ttggctgcca tattgcaaaa aatagacgcc ggggtctttt acagcactga aatcaaacgt
     2281 caggtaattg taacgcaggt accgacccca ggaatgaagc ggaccgcgca gcttctctac
     2341 ctgcctgccg tcttccgtta cctggtaaag cgttgcttcc tgcagcggca catcggactt
     2401 atccaattcg atgacggccg tcttgttctg ccccggagtg taacctgcct gggagaaccc
     2461 gatcaccggc ttccgtatcc aaccgggaat ggcattgggc tcgaggtacc agctgagtac
     2521 ctttcccgtc ttgcccgccg gaagcaaact gcgaacgatg aaccagccgt tctgaccgag
     2581 attcctgccg tcgaacaaca tcagatcggt gcctgtgctt cggatcagga ccttgcgttc
     2641 cgggtcttcg ggggcaagta ccagtgttcg cccggtggct aacggtgatg ggatgatgaa
     2701 ttcatggcgt cccctgtcat cgaaggtagt atgtcccgca aattgaggga tctttttgtc
     2761 cacggattcc atcttcgtat taccggacgg gtacaaggga aaattacccg ggtgaccgtc
     2821 ggcaaaatag gtcttttcaa aataagcggc cggcaggaat tcgagattga aaccggcatt
     2881 gcccaccagc ttttccggca acggttcatc caggtaaacg tcgatctcga cgcccttgtc
     2941 cttcgcggta acccggatcc gggaagtgaa attgtattct ttgtaggtta gcacggtctc
     3001 gatcgtgccg gcatcttttt tcacctgacg gctaaccatg accgggatca ggtcccactg
     3061 ctccggcgta ttttgaagcc ggatcgcgcc accggtcgcc gtcctcacac catggtggat
     3121 gatctcgaga cccgccgttt tctcatcgaa gaacatcccg ttatattgac tgctgaacac
     3181 cagcacattc actcccggtt tttcaaaata ccccgaatca ttgataagga gagggccggg
     3241 gtcggcgttt tgtgcaatag cgcgaacgcc gcatgcgagc gataccagga tggtgacggt
     3301 tgctatagtt accttcatat cgatctttta gttgacgcct gttttggtcc aatttacccg
     3361 tttgctttcg aaattggtgg tccctaaacc gatttcggcg aacaatgaga agttttgaac
     3421 agcgctgaca aaaaaccgtc agtttatggg tttgttcaga cctgttttcc ttttctgcgc
     3481 aatatagtcg gacgggagca tatcgaactt tgccttgaac gccctggcga aatagcctgg
     3541 tttggagaat ccggtctggt aggctatttc agaaatagtc agatcgcttt tttccaatag
     3601 tgtggccgct ttccccagtt tgaaggagcg aatatattcc acaggcgttt cgcctgtgat
     3661 ctcaagcagc ttgctgtaga gcgagctcct gctcatgccg acctgtctgc tgagaaaatc
     3721 aacggaaagt tgggagctga cgatattttc ttccaggcag cggacgacag cctgcaacag
     3781 acgttcgtcc tccgtctcga tttttatttc aggctgcatc attttgatct gtctgccata
     3841 cgtagtcgcc agatcacgcc gcagcgcaag aaggctcctt atctttgcat gcaatacttc
     3901 gaaattgaaa ggtttcgtaa tgtaatcgtt ggcccccgtc tcaaggccct gcaattgctg
     3961 cacttccccg gtcagcgctg tgagcaatat gatcggtatg tgacaggtgc gcttgtcggc
     4021 cttcagtttc ctggaaagta cgataccgtc catatgaggc atgctgatat cactgacgat
     4081 cagctgagga tgcaacgcta atgctttatg ccagccttct tttccgttgg tcgcctcgat
     4141 tatcctgtaa ttacccttca aattgtcctt taagtaaaaa cggaagtcgt cattgtcttc
     4201 aacgaggaga acgatgggtt tttcggaact atccaccgcc gaatcctccg ccggcgtctc
     4261 ccaccgttca ccggttcctt caagtatcgc cggcgatccg gcgccgttca cgacctgtaa
     4321 aggcagtcgc agggtgaacg tggtgccgcg gccgggctcg ctttccactc caatggtccc
     4381 gccatgcatt tccgcgaact cttttgcgat ggacaggccg atgccggtgc cctggttcaa
     4441 taccgcattg ccggacgcat gctgaaaaaa tcggtcgaat atccggtcca atttgtcagc
     4501 cggtatcccg atacctgagt cgatgactcc gatagagacc cacaaccttc cgtccggatc
     4561 cgggttgtcc gaagcattca tcgctacgga aatggttccc tcttccaacg tgaacttaaa
     4621 agcattcgag agaagattga acaggatgcg ttcgatctta tcgtgatcga agagcaaatg
     4681 aagacgcggg gtattgaagg taaagttcag ccggatatgc tttctcccgg agatgtccga
     4741 aaacgagtgt accacgtcat ctacaaaagc aaccaggtcg ccttcggtca acacgagtct
     4801 gagctcctgc tcctccatct ttctgaaatc cagcaactgg ttgacgaggt tcaggagtct
     4861 tctggcattc cgccggatca tgttcagctt gtccttagag ttatcttcct ggtgtcgtga
     4921 aagcagctcg ctgaccggag ccattatcag ggagatgggg gtcctgaact cgtggctaag
     4981 gttggtgagg aacttaagct tcatacggtc cagctcccgg gtccgtgccg cctccgcgcg
     5041 ctcgcgctcc agcaggaatt ttttcctgac tcttgaaagt ccccgatgac gactgtaggc
     5101 taagagtccg ccgatggcca atacataaaa aatataggca tacgcggtgc gccagaacgg
     5161 tggtcttaca tagattttta tcgtagtttc ttcggtgttc cagagaccgt cgttgttgct
     5221 tgcctttacg cggaacagat attcacccgg atcgagatta gtatacctgg ccgtgttcac
//
LOCUS       DASYSV010000436         6935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_859992, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000436 DASYSV010000000
VERSION     DASYSV010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859992"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="VGQ51_13390"
     CDS             complement(<1..1351)
                     /locus_tag="VGQ51_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9037620.1"
                     /translation="MKNILLSGILCLFIFPEIAFPQEYSYTHYTTANGLAGSTLYCIT
                     QDKDGFIWVGTEEGVSRFDGVHFRNFTTKDGLPDLEVLQIFGDSKGRVWMAPFSKAVC
                     FYYQGKIHNAQNDSTLSHLHLQQNAEGFHEDPHGNILIEERSALHIVKPDGTVVRLDS
                     LGGRQISDCVASATSAGGNFLAQVGASIIEFSTAGVLRKMPLNIQLQYRQPFYIAMNR
                     TLVAWRDSTNMYRIRAVSNGRLVTKYFDPNHSGHICFSTVGDSLVYWNGSSGSVECNY
                     FTGQTRQFLPGIRVSKAFRDGFGNLWFTSIGKGLFRLNSNDTRLLRLSGEKGQPANVL
                     ALTSLDNQLWVGDDQERIFRLSLPDMTSAGGHLFYKFANNRILYLGLAGKDKILAGSD
                     EGLLEGTREPRFLRTISTGVKSASRIDENTLLVGFSWGAAIVDLRNFAIDTLWHERAT
                     "
     gene            complement(1364..2110)
                     /locus_tag="VGQ51_13395"
     CDS             complement(1364..2110)
                     /locus_tag="VGQ51_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9037621.1"
                     /translation="MIRTILVDDEIDSILVLRRLLKTHCPDVEIVGEADAITKALDII
                     EHTSPDLLLLDIAIDTGNAFDLLNRLGNFNFQVIFVTAFNDRAIDAFRYSAVDYLLKP
                     VDGSLLQKAVEKAAGRMTEKQMTQNLKVLLDNTVALQVSNQKMAIPTLNGLTFVYFRD
                     ILRFEAEGSYTKVVLEDGRQIMATRSIKDYETLLPESIFYRVHHSHIIHLNKIREYRK
                     GRGGYIIMEDGTSIEVAIRRREDFLKRLMK"
     gene            complement(2337..2816)
                     /locus_tag="VGQ51_13400"
     CDS             complement(2337..2816)
                     /locus_tag="VGQ51_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037622.1"
                     /translation="MRFVHYSYSLALTLLSIGCRRTTDPKIAAIRADSILVSLHRTTH
                     YIDSARNLLVAEDPGGDSRHVASAVLLHTPLGDSLLSTTRTLVTLCHDSWPNPATIIN
                     IDNAFRFTKQLIAPHAWDQVYFSDTPTSTAYTLLFSMELECNSAANFAMSRIDGRRR"
     gene            2916..4586
                     /locus_tag="VGQ51_13405"
     CDS             2916..4586
                     /locus_tag="VGQ51_13405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9037623.1"
                     /translation="MKSSTGNKLVYGIVIALAGVFCIVVLSVWQYRRTQEEWAWVRHT
                     NEVLIGIGGINEARARYQLNFKDFLITGDSDFLHAAGNFSPILLERIRAVKGLTADNP
                     VQQRRFDSLIAITANDHGSRMQVLKEDELVSRLEAEERILLDRRREDSMTKASELQWI
                     LWGLIAAVLLLTFILFRKIRLDLRRQTTNLESTEEKYKTLFYKSPLPKWIYDENTMRF
                     LEVNDTAIRVYGYTREEFLGMTIADIRPPEDAERLRRNAPAVRGRVDVSHDTRWRHVK
                     KNGEIIDVEITSHSFTFEGHEARLVAVSDVSAYRRYEEQLRQLNDDLTKRAGELATSN
                     AELERFAYIASHDLQEPLRMVTSFLQLLKKRYGGRLDEKADQYIHFAVDGAERMKTLI
                     LDLLEYSRVGSGKEAFGKVDTGVVLKEMADIFQERIVIAGARIDIGPMPVVWGDKVQL
                     TQLFQNLLGNALKYHSDRKPLISIRAAEEAAFWQFAVEDNGIGIDPQFFDKIFVIFQR
                     LHTKGDYSGTGIGLAICKKIVDRHGGKIWVESVPEKGSTFYFTIAKKI"
     gene            4583..5011
                     /locus_tag="VGQ51_13410"
     CDS             4583..5011
                     /locus_tag="VGQ51_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9037624.1"
                     /translation="MKEIHILLVDDNEGDILLTREALEDARIINRISIAYDGLEAIRF
                     LKEPSEGEETPDLILLDINLPKMNGTEVLEVIKNDPQLKRIPVIMLTTSAAEKDILAS
                     YDKHANCYITKPVDLDRFMDVVRTIEDFWISIVKLPKANY"
     gene            5013..6446
                     /locus_tag="VGQ51_13415"
     CDS             5013..6446
                     /locus_tag="VGQ51_13415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV9037625.1"
                     /translation="MPPDNQPISVLVVEDNPGDLFLLEESLHTIDVLMEGIYHAATLA
                     SARQILAYRKIDLVFLDVSLPDSRGLDSYTGLQGLTQCAAVILLTGLNDRKLAMQALM
                     LGAQDYLIKGEFDEKLLSRAVRYSLERLRNLQHLRESEERYRGLFHNNPMPMWVFDTE
                     DFRILETNEAAVIHYGYTEEEFLGLTLADIYLDSDRHDLRREVDMAGRLRDGIKKGIH
                     ATMKKNGDVIFADIAWHWIGYKGRPAILELANDVTERVQLEKALNEQHVTRQRQITEA
                     VIVAQEKERTEIGKELHDNVNQILGASNLYINTAMTDEDMRQELLERSTELVSKAINE
                     IRKISKSLITPGLREIGLIESIEDIIEDIRVSKDAPAIDLDLVNVSEEQIEDRRKLTL
                     FRIVQEQLNNIVKHARATQVVIRLSLEGQYLVLSVVDNGIGFDEKKHRRGVGITNIIS
                     RAELFSGKVEIASAPGEGCVLTVSLPV"
     gene            complement(6493..>6935)
                     /locus_tag="VGQ51_13420"
     CDS             complement(6493..>6935)
                     /locus_tag="VGQ51_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV9037626.1"
                     /translation="DKIKSLNQSAPIILITPTPRGDFVYINNPKNNAYGSYKPKDGQT
                     LEQVAAAIADIGRHENLRVLDLYHDNRLSVPHTVRFKRLKDPASHAYRNYTYPDYTTI
                     PFDPATDDYPYPPEAAGMTFDGLHPSDAGNAEIAKQLSAILKHL"
BASE COUNT         1577 a         1660 c         2177 g         1521 t
ORIGIN      
        1 ctgttgcgcg ttcgtgccat agtgtatcga tggcaaagtt ccgcaggtcg acgatggccg
       61 cgccccagct gaagccgacc agcagcgtat tctcatcgat gcgtgaggcc gacttgactc
      121 ctgtggatat cgtgcggagg aaccggggtt cgcgggtacc ttcgagcagc ccctcatcgg
      181 accctgcaag gatcttgtct ttgccagcca atccgagata caggatccgg ttgttggcaa
      241 atttatagaa gagatgcccc cccgctgacg tcatatccgg gagtgagaga cggaagatgc
      301 gctcctggtc gtcccccacc cagagttggt tgtcaaggct ggtgagcgcg agaacgttgg
      361 ccggctggcc cttttctcct gacagccgga gcagcctcgt atcgttggaa ttcagccgga
      421 agaggccttt gccgatggac gtaaaccaga gattcccgaa gccatcccgg aaggccttac
      481 ttacgcggat gcccggcagg aattgccggg tctgtcccgt gaaatagttg cattccacgc
      541 tgcccgaaga tccgttccaa taaaccaggc tatcgcccac ggtggaaaaa cagatatgac
      601 cgctgtggtt cggatcgaag tacttcgtga cgagccttcc attcgaaacg gcgcggatgc
      661 ggtacatgtt agtggaatcc cgccaggcca cgagggtacg gttcattgcg atataaaatg
      721 gttgccggta ttggagctga atattgagag gcatcttacg gagcactccg gccgtcgaaa
      781 attctattat cgacgcccct acctgtgcca ggaaattgcc gccggcgctg gtggcgctcg
      841 ccacacaatc ggagatctgt cgaccgccga gcgaatccaa tcgtacaacg gtgccatccg
      901 gcttcacgat atgaagggcg gatcgttcct cgataaggat attgccatgc gggtcttcgt
      961 ggaagccttc ggcgttctgc tgcaaatgga gatggctcag cgtggagtcg ttctgtgcgt
     1021 tgtggatctt accctgatag tagaaacaga ctgccttgga gaaaggagcc atccatactc
     1081 tgcctttcga atccccgaag atctgcaata cctcgaggtc gggcaatccg tctttggtgg
     1141 tgaaattgcg gaaatggacg ccgtcgaaac ggcttacgcc ttcttcagtg ccgacccaga
     1201 taaagccgtc tttgtcctgg gtaatgcaat agagcgtcga accggcaaga ccattcgccg
     1261 tagtgtaatg ggtatagcta tattcctgcg ggaatgcgat ctccgggaaa atgaacaagc
     1321 aaaggatgcc cgaaagcagg atgttcttca tataatggac aggtcacttc attagtcttt
     1381 tcagaaagtc ctccctgcgc cggatcgcca cttcgatgga ggtaccgtct tccatgatga
     1441 tgtaccctcc tcttcctttc cggtattccc ttatcttgtt cagatgtata atatgggaat
     1501 gatgcacccg gtaaaaaatg gattcgggca gcagggtctc atagtccttg atggatcttg
     1561 tcgccatgat ctgccggcca tcttcgagga ccactttggt gtagctgccc tccgcctcga
     1621 accggaggat gtccctgaaa taaacgaagg tcagaccgtt gagcgtgggg attgccattt
     1681 tttgattcga cacctgcagg gctacggtat tgtccaacag gaccttcagg ttttgggtca
     1741 tctgtttttc cgtcatgcgc cccgccgcct tttccacggc cttttgcaaa agggatccat
     1801 cgaccggctt gagcagatag tccaccgcgc tgtaccggaa tgcgtcgatg gcgcgatcgt
     1861 tgaatgcggt cacgaagatc acctggaaat tgaaattacc caggcggttg aggaggtcga
     1921 aagcatttcc ggtgtcgatc gcaatgtcca gcagcagcag atcgggagaa gtgtgttcga
     1981 tgatgtcaag ggccttcgtg atcgcatcgg cttccccgac gatctcgaca tcaggacagt
     2041 gggtcttcaa cagccgtctc agaacaagga tactgtcgat ctcatcatct accagtatgg
     2101 ttcggatcat aaggttttat agagattcac aatataggga aataaagtta ttcagtgatc
     2161 agggggtgcg gggggtgatg tgctggcgcc ggcgggtgcg gaggatgatg gcgtgagcgg
     2221 gtgcggagga tgatggcgat gcgggtgcgg agggtgatgg cgtgagcggg tgcggaggat
     2281 gatggcgatg cgggtgcgga ggatgatggg ttggcgtctg cgggtgcgga atggggctat
     2341 cgtcgtcggc cgtcgatgcg ggacatggcg aaattggcgg cgctgttgca ttcaagttcc
     2401 atcgagaaga gcagggtata ggccgtcgag gtaggggtat cggagaagta tacctgatcc
     2461 caggcatggg gcgcgatcag ctgtttggta aaccggaaag cattgtctat gttgatgatc
     2521 gtcgcggggt tgggccagct gtcgtgacag agggtaacga gggtccgggt cgtggaaagc
     2581 agactatcac cgaggggggt atggaggagt acggccgagg ccacgtgccg gctatcgccg
     2641 ccgggatctt ccgccactag caggttccgg gcgctgtcga tataatgcgt cgtgcggtgc
     2701 agtgaaacaa ggatgctgtc cgcccggatg gcggcgatct tcgggtcggt ggttcgacgg
     2761 cagccgatgg aaagaagggt gagggcgagc gaatatgaat agtgcacgaa acgcatggac
     2821 ttttggattg cgcttcaaaa tacaccctaa tcgccatttt ttagtaaatt accgggtaat
     2881 cattttacct gatcctggaa gatctcctac caatcatgaa atcctctacc ggtaataaac
     2941 tggtgtacgg tatcgtcatc gcgcttgccg gtgtgttctg catcgtcgta ttgtccgtct
     3001 ggcaataccg gcggacacag gaggaatggg catgggtgcg gcacaccaac gaggtgttga
     3061 tcgggatcgg cggcatcaac gaggcgaggg cccgatacca gttgaatttc aaggatttcc
     3121 tgattaccgg tgacagcgac tttcttcatg ccgccgggaa tttctcccct atcctcctgg
     3181 agcgtatccg cgccgtcaag ggtctgacgg cggacaatcc tgtgcaacaa cgccggttcg
     3241 atagcctgat tgccatcaca gcgaatgatc acgggtcgcg gatgcaggtg ctgaaagaag
     3301 acgagcttgt atcacgactc gaggcagagg aacgcattct gctcgaccgg cggcgggagg
     3361 acagcatgac aaaggcatcg gaattgcaat ggatcttatg gggtcttatc gcggcggtgc
     3421 tcctgctgac cttcattctt ttccgcaaaa taaggctcga tctacggcgg caaaccacca
     3481 atcttgagtc tacggaggaa aaatacaaga cactttttta caagagtcca ttgccgaaat
     3541 ggatctatga cgaaaatacg atgcgttttc tcgaagtgaa cgatacggcc atccgcgtgt
     3601 atggttatac ccgagaggaa ttccttggga tgaccatcgc cgatatacgc ccgccggaag
     3661 acgccgaaag gctgaggcgg aatgccccgg ccgtccgggg aagggtcgat gtttcgcatg
     3721 acacccgttg gcggcacgtc aaaaagaatg gcgagatcat cgacgtggag atcacctcgc
     3781 attcctttac cttcgaagga catgaggcaa gattggtcgc ggtgagcgat gtctccgcct
     3841 acagaaggta tgaagagcag ctgcgacagt tgaacgacga cctgacgaag cgtgcagggg
     3901 agctggcgac gtcaaatgcg gagctggagc gtttcgccta tatcgcctcg cacgatctcc
     3961 aggagccctt acggatggtc accagcttcc tccagctgtt gaagaagcgc tatggcggcc
     4021 ggctggatga gaaggcggat cagtatatcc attttgcggt ggacggcgct gagcggatga
     4081 agacactgat cctcgatctc ctcgaatatt cgcgggttgg cagcggcaag gaagccttcg
     4141 gcaaggtcga taccggggta gtactgaagg agatggccga tattttccag gaaaggatcg
     4201 tcatcgcagg agcgcggatc gacattgggc cgatgcccgt cgtttgggga gacaaggtcc
     4261 agctgacgca gctgttccaa aacctgctgg gcaatgcgtt gaaatatcat tcggacagaa
     4321 aacccctgat cagtattcgc gcggctgaag aggccgcttt ctggcaattc gccgtggaag
     4381 acaacggcat cggtatcgac ccccagttct tcgataagat attcgtcatc ttccagcgtc
     4441 ttcataccaa gggcgactat tcgggcaccg gcatcggtct ggctatttgt aaaaagatcg
     4501 tggatcgtca tggagggaag atatgggtgg agtcggtgcc ggagaagggg agcacgtttt
     4561 atttcaccat cgcaaaaaag atatgaaaga gatccacata ttactcgtcg acgacaatga
     4621 gggggatatc ctgctgacga gggaagccct cgaggacgca cgtatcatca acaggatatc
     4681 catcgcctat gacgggctgg aggcgatcag gttcctgaag gagccgtcgg agggagagga
     4741 gactccggat ctgatcctgc tggacatcaa tcttcccaag atgaacggca ccgaggtgct
     4801 ggaggtcatc aaaaatgatc cgcagctcaa acgtattccc gtcatcatgc tcaccacatc
     4861 ggccgcggaa aaggatatcc tggcgtccta tgacaagcat gccaactgtt atatcaccaa
     4921 acccgtcgac ctggacagat tcatggacgt cgtcaggacg atagaggatt tctggatcag
     4981 cattgtgaaa ctgcctaaag ccaattatta atatgccgcc agacaaccaa ccgatatccg
     5041 tcttagtggt ggaagacaac ccgggcgatc ttttcctgct ggaggagtcg cttcacacta
     5101 tcgacgtgct tatggaagga atataccatg ctgcaacgct ggcgtcggca cggcagatcc
     5161 tggcttaccg gaagatagac ctggtcttcc tggacgtctc gctacccgac agccggggac
     5221 tggattctta cactggcctg caagggttga cgcagtgtgc cgcggtgatc ctgctgacgg
     5281 gtttgaatga caggaagctg gcgatgcagg cattgatgct gggggcgcag gattacctga
     5341 tcaagggcga gttcgatgaa aaattattgt cccgggcggt gcgttacagc ctcgagcgac
     5401 tgcgcaacct gcaacatctg cgggaaagcg aggagcggta tcgcggactt ttccataaca
     5461 atcccatgcc gatgtgggta ttcgataccg aagactttcg catcctggag acgaatgagg
     5521 cggccgtcat tcactatggc tataccgaag aagaattcct gggtctgacg ctggcggaca
     5581 tctatctcga ctcggaccgg catgacctga gacgcgaggt ggacatggcc ggccgtctgc
     5641 gcgacgggat caaaaagggc atccatgcga cgatgaagaa gaatggggac gtgatcttcg
     5701 cggatatcgc ctggcattgg atcgggtata agggtcgccc ggctattttg gagttagcca
     5761 atgacgtgac cgaaagggtt caattggaga aagcgttgaa cgagcagcac gtcacgcgtc
     5821 agcggcagat aaccgaagcc gtgatcgtgg cgcaggaaaa ggaaaggacc gaaataggca
     5881 aggagctgca cgataatgtg aaccagatcc tcggcgcgag caatctctat atcaataccg
     5941 ccatgacgga tgaggatatg cgccaggagt tgctcgaaag gagcaccgag ctggtcagca
     6001 aggcgataaa cgagatccgg aagatatcca aatcgctgat cacgccgggt ctgcgggaga
     6061 tcgggctgat cgagtcgata gaagacatca tcgaggatat acgtgtctcc aaggacgcgc
     6121 ccgcgataga cctggacctg gtaaacgttt ccgaagagca gatcgaggac cggcgcaagc
     6181 tgacgctttt ccgaatcgtg caggagcagc tgaacaatat cgtcaagcat gcccgcgcca
     6241 cgcaggtggt gataaggctt tcgctcgagg ggcaatatct cgtgctgtcg gtggtggata
     6301 atgggatcgg gttcgatgag aaaaaacatc gccgtggggt ggggataacg aatatcatca
     6361 gccgggcgga gctgttcagc gggaaggtgg agattgcgag cgcgccgggg gaagggtgtg
     6421 tgttgacggt gagtttgccg gtgtaagatg ggggtggcga ccgcatatct cgatggtggc
     6481 ggccgcctgt ggttaaagat gttttaggat ggcggagagt tgtttggcga tctcggcgtt
     6541 gccggcgtcg gaggggtgga ggccgtcgaa ggtcatgccg gcggcttcgg ggggataggg
     6601 atagtcgtcg gtggcggggt cgaaggggat ggtggtgtag tcggggtagg tgtagttgcg
     6661 gtaggcgtgg gatgcggggt ctttaaggcg tttgaaacgg acggtgtggg gtacggagag
     6721 ccggttgtcg tgatagagat cgaggacgcg gaggttttcg tggcggccga tatcggcgat
     6781 ggcggcggcc acctgttcga gggtctggcc gtcttttggt ttataggagc cgtaggcgtt
     6841 gttctttggg ttgttgatat atacgaaatc gccgcgggga gtgggcgtga tgagaataat
     6901 gggggctgat tggttgaggc ttttgatctt atcga
//
LOCUS       DASYSV010000437         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2266601, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000437 DASYSV010000000
VERSION     DASYSV010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266601"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VGQ51_13425"
     CDS             complement(<1..200)
                     /locus_tag="VGQ51_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037627.1"
                     /translation="MSAALTPPIIRPDQLSQLRGVYVYLDLASESDRETLRPLARDER
                     LWEFTKTLLITDTFDAQFDAYF"
     gene            complement(229..936)
                     /locus_tag="VGQ51_13430"
     CDS             complement(229..936)
                     /locus_tag="VGQ51_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9037628.1"
                     /translation="MTIKCILTDDEPFARKGLQGYIGQIDFLELVGVCENAVELNSML
                     KKEQVDLLFLDIEMPYMTGIDFLRQLAAPPRVVFTTAYEKYAVQGFDLEVLDYLLKPI
                     PFERFLRSANKAFDYFRLQQASATPGDYIFVKSDSRLEKIAFADILFAEALENYVAIY
                     TREKKIITHLTLKMLQEKLPAISFTQPHKSYLIAIDKIGSIEGNILHIADYQVPISKY
                     LKDSIMEKIVNSRLLKR"
     gene            complement(933..1970)
                     /locus_tag="VGQ51_13435"
     CDS             complement(933..1970)
                     /locus_tag="VGQ51_13435"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9037629.1"
                     /translation="MKFFWKYIFPPLYGLFFYTILRLVSDLTTEVEFWQRRREQNAVE
                     IAASILLGYGLVRLPNYFVRRFEKKSRANSLRSITLEFGEILLVAMAIATTACVIVHI
                     LAHDYPIGIDDLTIVNVLLSLFLLLHYSIVRGNSFIRAYIEQKVLMEKVRNDHLQTEL
                     KFLKAQYHPHFLFNALNTIYFQMDEDVSAAKKSVEKFSGLLRYQLYDQQQTVPVSQEI
                     RYLQNFIQLQQTRTSEKLRLDTHFDEHLDGQRVYPLLFLPLVENAFKYVGGDYRLCIS
                     ATFCEDQIQFHVVNSIPAAIDGPERKDNGIGLENLKRRLELLYPGKHSFTIEKKDGDF
                     SAALSIQLPPA"
     gene            complement(1992..>3105)
                     /locus_tag="VGQ51_13440"
     CDS             complement(1992..>3105)
                     /locus_tag="VGQ51_13440"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEV9037630.1"
                     /translation="MIMLVLSMHAAVTYSGLGGWYYKEGPQPGKPELVVFATYQAFLQ
                     SFFMGLLFFIAGYFVPGAYDRKGARRFLRDRAFRLGWPSLLYIFVIGPLTEFYVARSW
                     AGDSPDRSFVQEYGHYITGLRFPGGSGPLWFCVALLIFCCVYAGWRTLTVRRTKSNTS
                     TGTPRPFPGTGTITVFILSIATASFLVRIPWPNGTDFYNMQFCDFSQYIAFFIAGTLA
                     YRCSWLTTLPDATGNRWGFFGRIGGPVCWFALLIGGGALQGHAGDLSGGMHWQSMGMC
                     LWEAMVGTALSIWIIALFRKRFNGQGRWARFFSDNAFAVYVFHPPMLISITLGMSGVP
                     APALLKFLLATVLSIIVSFAVAALVIRRIPVLKRIV"
BASE COUNT          743 a          798 c          895 g          669 t
ORIGIN      
        1 tcgaaatagg cgtcgaactg cgcgtcgaaa gtatcggtga tcaggagcgt tttggtaaat
       61 tcccagagcc gttcgtccct ggccagcggc cgcaaggttt ctctatcact ctcgctggca
      121 agatcgagat agacatagac gccccttaat tgcgaaagct gatcgggccg gatgatggga
      181 ggagtaagtg cggcggacat accagttcgt ttcggtaaag atagcgattt accgcttcag
      241 caaccggcta ttcacgatct tttccatgat cgagtccttc agatatttgc tgatgggcac
      301 ctggtaatcg gcgatgtgca ggatatttcc ttctatcgaa ccgatcttgt cgatggcgat
      361 aagataggat ttatgcggct gcgtaaagga gatggccggc agcttttcct gcagcatttt
      421 cagggtcagg tgggtgatga tctttttttc gcgcgtgtag atggctacgt agttctcgag
      481 cgcctccgca aacaggatat ccgcaaaggc tatcttttcc agccggctgt ccgacttgac
      541 gaagatatag tcgccgggag tcgccgatgc ctgctgcaac cggaagtaat cgaaggcctt
      601 gttcgccgac cggaggaatc gctcgaaagg gataggcttt aagaggtagt ccagcacctc
      661 caggtcgaat ccctgaacgg catatttctc atacgcggta gtaaagacga cacgtggcgg
      721 agcggccagt tgccgcagaa agtcgatgcc cgtcatatac ggcatctcga tgtccaggaa
      781 gaggagatcg acctgctctt ttttcagcat cgaattcagc tcgaccgcat tctcgcaaac
      841 cccaaccagc tcgaggaaat cgatctgccc gatatagccc tgtaaaccct ttcgtgcaaa
      901 aggttcatcg tcggtgagta tacatttgat ggtcatgcgg ggggcagttg tatcgaaagg
      961 gccgcggaga agtcgccgtc ttttttttcg atggtgaagg aatgtttgcc ggggtacagg
     1021 agctcgagcc gtcttttgag attttcgaga ccgatgccgt tgtcctttct ctccggcccg
     1081 tcgatcgcgg caggaatgct gttgacgaca tggaactgga tctggtcttc gcaaaaggtc
     1141 gcgctgatac ataaccgata gtctccgcct acgtatttga aggcgttctc caccagcggc
     1201 aggaagagca gcggatagac ccgctgaccg tcgagatgct catcgaaatg cgtgtccagc
     1261 cgcaatttct ccgaagtccg cgtttgctgc agctgtataa aattctgcag gtaacggatc
     1321 tcctggctga ccggcaccgt ttgctgctgg tcgtataatt ggtatcggag cagcccggag
     1381 aacttctcga cgcttttttt ggccgccgat acgtcctcgt ccatctggaa atagatcgtg
     1441 ttaagcgcgt tgaacaggaa atgggggtgg tattgcgcct tgaggaactt cagctccgtt
     1501 tgcagatgat cgttccggac cttttccatc agcacttttt gctcgatata ggcgcggata
     1561 aagctgttgc cgcgcacgat agaataatgc agcagcagga atagcgaaag caacacgttc
     1621 acgatcgtca gatcgtcgat gccgatgggg taatcatgtg ccaggatgtg tacgatgaca
     1681 caggcggtag tggcgatggc catcgctacg agcaggatct cgccgaattc gagggtgatg
     1741 gagcgaagac tgttcgcccg gctctttttt tcgaatctcc ggacgaaata attgggcagt
     1801 cttaccaatc catatcccaa caggatggaa gccgcgatct cgacggcatt ctgctcccgg
     1861 cggcgctgcc agaattcgac ttcggtggtc agatcggaca ccagccgcag gatcgtgtag
     1921 aaaaacaatc catacagcgg ggggaagata tatttccaga aaaatttcat gtcgctgggt
     1981 aaggtacgat tttagacaat tcgtttcaac accggtatcc ttctgatgac aagcgcggcg
     2041 acggcaaacg aaacaatgat gctcaggacc gttgccagca ggaatttgag cagggcggga
     2101 gcggggacgc cggacatacc cagcgtgatg gagatgagca tcggcgggtg gaacacatag
     2161 acggcaaagg cattgtccga gaagaacctg gcccaccggc cctgcccgtt gaatctcttg
     2221 cggaaaaggg cgatgatcca gatggaaagg gccgtcccca ccatggcttc ccaaagacac
     2281 atgcccatgc tctgccaatg catgcctccg ctgagatccc cggcgtgtcc ctgtagtgcg
     2341 ccaccgccga tcagaagggc gaaccagcat accggtcctc cgatcctccc gaaaaatccc
     2401 caacgattgc ctgtggcgtc cggcaaggtc gtcagccagc tgcagcggta ggccagcgtg
     2461 ccggcaatga aaaaggcgat atattgggag aaatcacaga attgcatatt gtagaagtcg
     2521 gtgccattgg gccacggtat cctgaccagg aagctcgccg tggcgatcga taaaataaag
     2581 acggtgatgg ttccggttcc ggggaatgga cgcggcgtgc cggtagaggt attcgacttt
     2641 gtccgtcgca cggtgagcgt acgccagccg gcatacacgc agcaaaagat cagaagggcg
     2701 acgcagaacc atagcggacc gctgcctccg ggaaagcgaa gaccggtgat ataatgaccg
     2761 tattcctgaa cgaagctgcg atccggcgaa tccccggccc aggatcgggc aacgtagaat
     2821 tcggtcaatg ggcctataac gaagatatac agcagggaag gccatccgag acgaaaagcg
     2881 cgatcccgga gaaagcgtcg tgcgcctttt ctgtcatagg cccccggtac gaaatagcct
     2941 gcgataaaga aaagaagacc catgaaaaag gactgcagga aggcctggta agtggcgaac
     3001 acgaccagct ccggcttacc gggctggggc ccctctttat aataccaacc gccgagaccg
     3061 ctgtaggtaa ctgccgcgtg catgctgagc acgagcatga tcatg
//
LOCUS       DASYSV010000438         4948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_986682, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000438 DASYSV010000000
VERSION     DASYSV010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986682"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VGQ51_13445"
     CDS             <1..296
                     /locus_tag="VGQ51_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9037631.1"
                     /translation="RTKEIGVRKVLGAGVISLWALLSKDFLRLVVLSMAVAMPLMVLL
                     MNKWLQNYAWHTPLSWWIFASAGAGILLITLATVSFQSLKAALANPIRALRTE"
     gene            complement(320..1603)
                     /locus_tag="VGQ51_13450"
     CDS             complement(320..1603)
                     /locus_tag="VGQ51_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV9037632.1"
                     /translation="MSKPKLLLLFALLWASFAYCQKTIIYCGRLIDPKAGQVLTEMTI
                     VVTGNSITAVEKGYAAAATGDKVIDLKNRTVMPGLIDAHVHLEDQTSPDRQLRGFMQN
                     MADIAFLSAVYARTTLMAGFTTVRDVGGRGVNISLRNAINKGLTVGPHVFTAGKIISS
                     TGGHADPTHGYRDGLRVDPEFTETVVDGKDECIKAVRQMYKEGADLIKITASGGVLSL
                     EPNGTGAQFSDEELKAIVETAKDYGMRVAAHAHGAEAIKRAIRAGVTSIEHGTYMDDE
                     AIALFKQHGTWYVPTIIAGKSVADSAKKPGYYPPVVTAKALAVGPVIQGTFAKAYKAG
                     VKIAFGTDAGVYAHGKNWMEFIYMTEAGMPALEAIKCATVNASELIGVSDKTGTIEPG
                     KWADIVAVEGDPTKDIHAMGQMRFVMKEGVVYKNE"
     gene            1684..1833
                     /locus_tag="VGQ51_13455"
     CDS             1684..1833
                     /locus_tag="VGQ51_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037633.1"
                     /translation="MKRLTPILLLLLLLPLSLFAQPPREWAAFNHDLPRRHAYRTIRP
                     SCRLQ"
     gene            complement(1913..2548)
                     /locus_tag="VGQ51_13460"
     CDS             complement(1913..2548)
                     /locus_tag="VGQ51_13460"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV9037634.1"
                     /translation="MSLRLGDKAPNFKAATSIGEIDFYEFLGDSWGILFSHPADYTPV
                     CTTELGKTAALKNEFAKRGVKVLALSVDPVDKHLGWINDINETQNVNVDFPIIADEDR
                     KVSTLYEFIHPNASATATVRSLVIIGPDKIVKLIITYPASTGRNFQEVLRVVDSLQLT
                     AKHSVSTPADWKQGEDVVVANTISTEDAEKRFAGGVKVVKPYLRYTAQPKA"
     gene            complement(2762..3988)
                     /gene="dinB"
                     /locus_tag="VGQ51_13465"
     CDS             complement(2762..3988)
                     /gene="dinB"
                     /locus_tag="VGQ51_13465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV9037635.1"
                     /translation="MSQPQRYIAHFDLDAFFVSVECLNEPSLKGKPLLVGGHSDRGVV
                     AACSYEARKFGIHSAMPMRTALRLCPHATVVGNARGEYSKYSRWVTEIIAAKAPLFEK
                     ASIDEFYLDLTGMDRFYDPYGWTIALRDEIISATQLPISFALASNKMISKIATQEAKP
                     NGYIQVPPGMEQEFLAPLKVNKIPGVGEQTWAVMQEMGIITIRDLSEQPIEVLEKRFG
                     KYGNDLWHKARGSHDSEVVPYHEAKSISTENTFEENKTDMDFLMAELVRMTERVAYEL
                     RQDDKTTGCIAVKIRYPDFETTSRQTTIPYTFYDDELIVQAKELFHKMYRKGQPIRLL
                     GVRLSELTSEAIQTNLFVDTRRKTELYKAIDDVKGRFGKDAVVKAGGQAASDGDRSVG
                     KATSRFLRRSGEKEGE"
     gene            complement(4010..4267)
                     /locus_tag="VGQ51_13470"
     CDS             complement(4010..4267)
                     /locus_tag="VGQ51_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037636.1"
                     /translation="MKRLTVRSSADRLEIRSGSAYPGAGYRRGWHVPGPIPPDAEDKI
                     EGLTKGIRIPEQGENLFENAGAQVFAYYDEHKREIVLIGIH"
     gene            complement(4271..>4948)
                     /locus_tag="VGQ51_13475"
     CDS             complement(4271..>4948)
                     /locus_tag="VGQ51_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037637.1"
                     /translation="GKKVGFRKITHNNAQSMDAEHAYVDNSMLLDGKLYFVSAEDNRL
                     NVQIRDFNSGKLLHEYSAGRDDSITFKNTPIIQEGSFYHPGARELGKTRQLLRKMVSG
                     NAVLLATREDSGRVSLTIGAWQMMQRSGGFGAGPMMPGAPVFMSPGVYFRSSNVRSSR
                     FKMQLDSATLQYVPGDISADIGDRIEQYTDKISIPPQGENLFTNDGKYIYAFYNRDEH
                     KIMLTSF"
BASE COUNT         1018 a         1615 c         1097 g         1218 t
ORIGIN      
        1 aacggacaaa agagatcggc gtccgcaaag tcctgggtgc cggagtaatc agcctgtggg
       61 cgctattgtc gaaagatttt ttgcgactcg tcgtattgtc catggctgtc gccatgcccc
      121 tcatggtcct gttgatgaat aaatggctgc agaactatgc ctggcacacg ccgctgtcct
      181 ggtggatatt cgcctccgcc ggtgccggca tcctgctgat cacgctggcg accgtgagtt
      241 tccaatcgct gaaggcagct ctcgccaacc ccatccgcgc cttacggacg gaataaccat
      301 ttcgtgcaca tccaaaatcc tattcattct tataaaccac cccctctttc attacgaacc
      361 gcatctgccc cattgcatgt atatccttcg tcggatcgcc ttccaccgcc acgatatcgg
      421 cccatttccc cggttcgatc gtccccgtct tatcgctcac gccgatcagc tccgaggcat
      481 tcacggtcgc gcatttaatg gcctccagcg ccggcatgcc tgcctccgtc atataaatga
      541 actccatcca gttcttgcca tgcgcataca cgcctgcatc cgtgccgaat gcgatcttca
      601 cgcccgcctt ataggccttc gcaaaagtcc cctgaatgac gggccctaca gccaacgctt
      661 tcgcggtgac caccggcggg taatatcccg gttttttagc gctgtcggcc acgctctttc
      721 cggcaatgat ggtcggcaca taccaggtgc catgttgttt aaagagcgct atcgcctcat
      781 cgtccatata cgtgccatgc tcgatcgagg tgactcccgc acggatcgca cgtttgatcg
      841 cctcggcccc atgcgcatgt gccgcgacac gcattccgta atccttcgcc gtctccacga
      901 tcgcctttaa ttcctcatcg gagaactgcg cgccggtgcc gttcggctcc aggctgagca
      961 ccccgccgga tgcggttatc ttgatcagat cggcgccttc tttatacatc tgccgcaccg
     1021 ccttgataca ttcgtccttt ccatccacca cggtctcggt aaattccggg tcgacacgca
     1081 gcccatcgcg atagccgtgg gtgggatcgg catgaccgcc cgtcgatgaa atgatcttgc
     1141 cggcggtaaa cacatgcggg cccaccgtca aacccttatt gatggcgttt cgcaacgaga
     1201 tattgacgcc ccttcccccg acatccctta ccgtggtgaa acccgccatc agcgtagtcc
     1261 ttgcatatac tgccgaaaga aacgcgatat ccgccatatt ctgcatgaat ccccttagct
     1321 gacggtcggg gctcgtctga tcctccaggt gcacatgcgc gtcgatcaac cccggcatca
     1381 ccgtcctgtt cttcagatcg atgaccttgt cacccgtagc cgcggccgca tatcccttct
     1441 cgacggcggt aatgctgttc cccgtgacga cgatcgtcat ttcggtcagc acctgtccgg
     1501 cctttgggtc gatgagacgg ccgcaataaa tgatggtttt ttggcaatag gcgaaagacg
     1561 cccatagcag tgcgaatagc agtagtagtt taggcttgct cacgggcata ataaattttt
     1621 agaggtgttc gcgaatgctt cgtatttcat ctctctaata taagcaattc ttatcttgtc
     1681 tgtatgaaac gcctcacgcc catcctcctt ctcctcctcc tcctccccct atccctcttc
     1741 gctcaaccac cccgcgaatg ggccgccttc aaccatgatc ttccccggcg gcacgcatac
     1801 cgaaccatcc gaccatcctg ccgtcttcaa taaggccgtc gaagaattcc taaaataaca
     1861 aaagggcccg ccggcttcgc cggcgggccc gaacccattc aataaacctt ttttatgctt
     1921 tcggctgcgc cgtataccgc agatacggtt tcaccacctt cacgcccccc gcgaacctct
     1981 tctccgcatc ttccgtactg atcgtattgg cgacgaccac atcctcaccc tgtttccaat
     2041 ctgccggagt agacacactg tgtttcgcgg tcagctgcag cgaatcgacc acccgcagca
     2101 cctcctggaa gttcctgccc gtagaagccg gatacgtgat gatcagcttc acgatcttgt
     2161 ccggcccgat gatgaccagg ctgcggaccg tagccgtagc agatgcattc ggatgtatga
     2221 actcatacaa cgtgctcacc ttccggtctt cgtcggcgat gatcgggaaa tccacattca
     2281 cattctgcgt ttcattgata tcgttgatcc agcccagatg tttatccacc gggtccacgc
     2341 tcagggccag caccttcact cccctcttgg caaactcgtt cttcaacgcg gccgtcttac
     2401 ccagctcggt agtacacacc ggcgtatagt cggcagggtg agaaaacagg attccccagg
     2461 aatcacccag aaattcgtag aagtcgatct cgccaatcga tgtagcggct ttgaagttgg
     2521 gagccttgtc cccgagacgt aaactcatat aatttaaaat ttttgcccgc cgggctcacg
     2581 gcccgtttgg gagggtaaag atattaattc cctatcaatt cagtggactt tgctgttaaa
     2641 ttttttatgc cctcttccta actcgctgat tttcttcgcg gttcacgccc gctttcccgg
     2701 tatcacgccc cctctttctc tcccgatctc cgccctcacc gccactcccc gctgcccggt
     2761 atcactcccc ctctttctct cccgacctcc tcaaaaatct cgaagtcgcc ttccccaccg
     2821 accgatctcc atccgacgca gcctgtccgc ccgccttcac caccgcatcc tttccgaacc
     2881 tccctttcac atcatcgatg gccttataca gctcagtctt ccgccttgta tccacaaaca
     2941 aattcgtctg tatcgcctcc gacgtcagct cgctgagccg cacacccaac agcctgatcg
     3001 gctgcccctt ccggtacatt ttatggaaca actccttcgc ctggacgatc agctcatcat
     3061 cataaaaagt ataaggtatc gtcgtctgcc gcgacgtcgt ctcgaaatcg ggataccgta
     3121 tcttcacggc gatacatccc gtagtcttat catcctgccg cagctcatat gccaccctct
     3181 cggtcatccg caccagctcg gccatcagaa aatccatatc ggtcttgttc tcttcgaacg
     3241 tattctccgt cgagatcgac ttggcctcat gatagggcac gacctcgctg tcatgactac
     3301 cgcgtgcctt gtgccaaaga tcgttcccat atttcccgaa acgcttttcc aaaacttcta
     3361 tcggctgctc gctcaaatca cggatcgtga tgatgcccat ttcctgcatc accgcccatg
     3421 tctgctctcc tactcccggt atcttgttca ccttcaacgg ggcgaggaat tcctgttcca
     3481 tcccgggcgg cacttgtata tagccgttgg gtttcgcctc ctgagtggct atcttcgata
     3541 tcatcttgtt cgaggccagc gcaaacgaaa taggaagctg tgtcgccgaa atgatctcgt
     3601 cgcgcaacgc gatcgtccat ccatacgggt cgtaaaatct atccataccc gtcaggtcca
     3661 ggtagaactc atcgatcgaa gccttttcga acaggggcgc cttcgcggcg atgatctcgg
     3721 tcacccaacg cgaatacttg ctgtactcac cccgcgcatt ccccaccacc gtcgcatgcg
     3781 gacacaaccg gagcgcggtc cgcataggca tcgccgaatg gataccgaat ttccgcgcct
     3841 catagctgca cgcggccacg accccccggt cgctatggcc cccgacgagc aatggcttgc
     3901 ccttcaatga cggctcattg agacattcga cgctcacaaa aaaagcatcc agatcgaaat
     3961 gggcgatata acgctggggc tgcgacaccc tgcaaagata ctaatttttt tagtggatgc
     4021 cgatcaggac tatctccctc ttatgctcat cataataggc gaacacctgc gcacccgcat
     4081 tctcgaataa attctctccc tgttcgggta tcctgatccc cttcgtcagc ccttcgatct
     4141 tgtcttcggc atccggtggg atgggcccgg gtacatgcca cccccggcga tatccagcac
     4201 ccggatatgc agatcctgat cggatttcaa gtcggtctgc tgaagaacga acagttaggc
     4261 gtttcatatt tcaaaaactt gttaacatga tcttatgttc atctctgttg taaaaagcat
     4321 aaatatattt cccgtcattc gtgaacagat tctccccctg cggcggaatg ctgatcttat
     4381 cggtatattg ttcgatgcga tccccgatat cggcgcttat gtcacccggg acatactgca
     4441 gcgtcgccga atccagctgc atcttgaagc gtgaggaacg gacattactg cttcggaagt
     4501 acactcccgg cgacatgaac accggcgcac ccggcatcat cggccccgcc ccgaacccgc
     4561 cgctccgctg catcatctgc catgcgccga tggtcagact gacccgtccg ctgtcttcac
     4621 gcgtagcgag gagcacggcg ttcccgctca ccatcttccg gaggagctga cgcgtcttgc
     4681 ccagctcgcg cgcgcccggg tgataaaaac tgccctcctg gataatggga gtattcttga
     4741 aagtgatgct gtcatccctt cccgcactgt attcatgcaa aagctttccg ctgttgaaat
     4801 ccctgatctg tacattcagc ctgttgtcct ctgccgatac aaaatacaat ttgccgtcga
     4861 gcaacatgga attgtcgaca tacgcatgct ccgcatccat cgattgcgca ttattatgcg
     4921 tgatcttccg gaaacccacc ttcttccc
//
LOCUS       DASYSV010000439        10788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_472455, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000439 DASYSV010000000
VERSION     DASYSV010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472455"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VGQ51_13480"
     CDS             <1..672
                     /locus_tag="VGQ51_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9037638.1"
                     /translation="KIADRRYEVSPGQCFILPQNTEHAYGSSEHDPWTIYWLHFGGNV
                     LSSFNAMPIIQKHFRPHAVRSSNEMITLFSKIYKTLELGYSIDNLLFANMCLSHFITL
                     FIYNQKHFAASPSEKLDCIDSAILFMQEHINENITLNDLSHRYNYSPSRFSSLFKQRT
                     GYAPIDYFIQMKMQKASQHLGITDKSVKDIALSMGFDDPYYFSKRFTKVMGMSPTKYR
                     SVTRD"
     gene            complement(750..1226)
                     /locus_tag="VGQ51_13485"
     CDS             complement(750..1226)
                     /locus_tag="VGQ51_13485"
                     /inference="COORDINATES: protein motif:HMM:NF025425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HEV9037639.1"
                     /translation="MKRLFLFAGVALLSLRAFAQSADEKAAAIKDMVESKNFIFQAQT
                     ALPMRGNVRHLTTEYDLKVTPTAVVSELPYFGRAFVAPMNPTQSPLQFTSTKFDYTAT
                     TRKSGGWEIVIKPTDHREVQSMTLTISTAGYTTLQVTNLNRDPITFNGDITAPRKK"
     gene            1275..2705
                     /locus_tag="VGQ51_13490"
     CDS             1275..2705
                     /locus_tag="VGQ51_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV9037640.1"
                     /translation="MANSEFDAVVVGSGPNGLAAAILLQQQGLSVLLIEAKSDIGGGL
                     RSAGLTLPGFVHDICSSVHPLGIASPFFSSLPLKDHGLEWVESPIAAAHALDGGRAVC
                     LMRSVAATARGVGADENSYRRLLDPLVEDWPELMPQILGPLRFPKHPLMMARFGLEGL
                     RSVAALARRFDTMEARGLLAGMAAHAMQPLTNLTTAAVALLFLLAGHGKGWPFPKGGS
                     ARLANALADYFRKLGGVIQTGSPVTDLGQLPPAKALLLDLTPRQLLQLGGHRWTSLYA
                     WQLERYRYGMGVFKIDWALAEPTPFPAMDCRQAATVHLGNSFEEIAAGEAGTIRGVHA
                     EKPFVLFTQTSLFDPSRAPAGKQTAWAYCHVPGGSRRDMTDVIESQVERFAPGFRDRI
                     LARHVMDSAQIESYNPNYVGGDINGGIMDIGQLFTRPVLRRSPYRTSAKGIYICSSAT
                     PPGGGVHGMCGYYAAKRALRDVFGIR"
     gene            complement(2751..4316)
                     /locus_tag="VGQ51_13495"
     CDS             complement(2751..4316)
                     /locus_tag="VGQ51_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037641.1"
                     /translation="MANRFSDTLFQLIHSLEKSEKRHFKLYIKRSSTKEDLKIVQLFD
                     AMDKLTDYDEKNLLKKLPGIEKPQLSNLKTHLYKQILASLRLLKSADSIDLQLNEMFD
                     YAHILYKKGLFQQSLRMLDRAKETAKANQKFNFLVQVVALEKRIETLHITHSMQMRAE
                     QLSAESKSVISRIDMVARLSNLALLVYSWYIHNGHSRNEKDEAALREYLREQLPPNAW
                     EQTGFYERLYLYQSHNWYAFIRQDFLMYYRYSQKWLDLFEEQPLMIRVETGHYIKALH
                     NLLNAHFDLRNHERFARILQQFENFSQTDRVSENDNFRIQSFVYITTARINQVFMQGN
                     FAEGLAMVPGIEEKLAEYDLFIDRHRILVLNYKIATMYFGAADHATCIDYLQRIINDP
                     TDLRNDLQCYARVLHLMAHYELKNFDLMESLTKSVYRFMARRERLTKVEEEMFRFLRT
                     SFQTSRTGLRNEFEKFLEKIKGFEGNRYEARAFAYLDLVSWVESKVYQRPMSEIISEK
                     YRKSKRRVRMTEA"
     gene            4753..5139
                     /locus_tag="VGQ51_13500"
     CDS             4753..5139
                     /locus_tag="VGQ51_13500"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9037642.1"
                     /translation="MQSFFFIRREGRYVKVDFRNIVYIEAKKNYTRLMLEDKSTVLVL
                     GTLKQWERLLPCDLFCRVHRGYIVSIEKIVAFDNKHVYLPGSEIITIGDQYRNALPDS
                     VTILAQEPAHSPARSVRKDVLSELEL"
     gene            5503..6018
                     /locus_tag="VGQ51_13505"
     CDS             5503..6018
                     /locus_tag="VGQ51_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9037643.1"
                     /translation="MLYDQYYGLCLKIAFRYVNTYEQATEVTHDAFLKMFRNFSRFEV
                     RDKEKVEMLLIGWIRRVVINAAIDYMRREMNNTQTYPIPEHVWEHKDDGQSSDNGVLY
                     KELIALVKDLPPAYRIVFNLHVIEGYSHPEIAKMLGITTGTSKSNLSKARAHLQKALT
                     AEKSEKILCQI"
     gene            6006..7361
                     /locus_tag="VGQ51_13510"
     CDS             6006..7361
                     /locus_tag="VGQ51_13510"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV9037644.1"
                     /translation="MSNLKDTEVDDLIRRASDKYPLRTDSADWIKVAAALDMDPVPAG
                     TGDGDDKRRKRRFFWLLLLLPLGGAGYYTLTHTGNVAGQAGHAAVAVVAGQTGHPAAA
                     VTTGQTGHSAAAITTGQTSRQDVGGTAKNVPGAGLQDVGRTAKNIPGSKGVDGKAVRM
                     DRQVKDGTPASGNSVAADRQFNNGSAGAVNPNRAPDPGRDLATAPQPYIATAPLSLEW
                     AGDMQRAHIPHDYRLSVNVTAPPPAKNTDPAKPKKQKTAYLYAGILGAPDISTVKMQS
                     VKGIGSTFGVLVGYAFNPRWSIETGAYLDRKKYYTEGEYFNKSNLNLYGGTMLNADGT
                     CSMWEIPLNVRYTFNPNGKTKFFATAGVSTYLMTKENYTYAYQSALGWQGQKTYNNKN
                     PSKYPFSIFNLSFGFEQRLGRVGSLRLEPYLRLPLEGIGTGKLPIMSAGVNIGITRRL
                     W"
     gene            7385..7810
                     /locus_tag="VGQ51_13515"
     CDS             7385..7810
                     /locus_tag="VGQ51_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037645.1"
                     /translation="MRKKRTIIWIGVLLLVLVMAGWGWYLFDKPHRSAAAYSADVTID
                     ADTLYHQYKADEHAADLKYVGKVLSVAGRLTEIQRSGNSEIWILSIQPGGGGINCELF
                     AGTKVDPEPKPGDAVTIKGRCTGFLITDVNLADCVPDNK"
     gene            7859..8233
                     /locus_tag="VGQ51_13520"
     CDS             7859..8233
                     /locus_tag="VGQ51_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037646.1"
                     /translation="MNRKFLLATLCIGAIVFYLAGCSKESADKLSGSATCDTASVSYS
                     TQIVDILQNNCYSCHSGATPPTGIRLDTYANLKVFVDNGFLVSAVTQNGVVTPMPYGL
                     PRLPACEVNTIVAWVHQGAPAN"
     gene            8255..8797
                     /locus_tag="VGQ51_13525"
     CDS             8255..8797
                     /locus_tag="VGQ51_13525"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV9037647.1"
                     /translation="MKQLFIALFLYLAFFRPSTAYSQMYSTRTGFIGFYSKTALEDIK
                     AENNQVYAIIDAGKKNVALAVLLKGFIFTKELMQEHFNENYVESDKYPKATFTGAYTG
                     AVGGDGVYKVTIKGNLTLHNVTKPIESEATIEIKNGHLLGKTEFKVRPEDFNINIPSI
                     VRDKIDKEITVKVNFDCSSK"
     gene            8810..9703
                     /locus_tag="VGQ51_13530"
     CDS             8810..9703
                     /locus_tag="VGQ51_13530"
                     /inference="COORDINATES: protein motif:HMM:NF040196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HEV9037648.1"
                     /translation="MNPQRIFFLLLSLAAATGLRAQDSTLLNMLSDSMSAHKGRSYVT
                     GTFKATHIINTQTIESPAKNNLNFVIQHRFGTLNSGAYNFFGLDNATLRLGLDYGITD
                     RLAVGIGRSSYLKTFDGYLKYKLLRQTESAGMPVSVSLLGAITDYTQDFPEETYLNMK
                     YRTAYAGELLIARKVSSHLSLQVTPTWLHYNLVPTSNDKNDVFAVGLGGRMKITRRMS
                     IDAEYDIVPSGQVVSTTTHNSFSLGWDIETGGHVFQLVFSNSQSMVESQYLTQTTGTW
                     GKGDIYFGFNISRNFNLSKKR"
     gene            9700..>10788
                     /locus_tag="VGQ51_13535"
     CDS             9700..>10788
                     /locus_tag="VGQ51_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037649.1"
                     /translation="MIRKILFILTPGERRQGAFLACGNAFISVLDICGLGALLYLVAF
                     YTGQGVDTRLSWLPAWLRDRNSLAPISVFSLLFLVKSAGGYWMLQSQYRYVYRVASRI
                     SESNLLRYLEGSYTDYVRVDASVQIRRISQQPIEFCQYILAGLQQLVTEGSLVALSIA
                     GIVWYNSRLFLLLLCLLVPAVSILASFSRRRLKSVRRDIKSSGEKSLQHLREALGGYV
                     EGNLYDSISFFARRYGRRQADLNEHLSVLQILQGIPSRLIEAFAVFGLFLLIVFARWT
                     GNAQAASAITLGAFLAAAYKIIPGLVRMFNITGQIMTYRFTVEDLVTEARKPQRMSVP
                     AVPSIRQITLRNVEFSQPQGRVVKGLNLC"
BASE COUNT         2476 a         2789 c         2985 g         2538 t
ORIGIN      
        1 aagatcgcag accggcgtta tgaggtttcg ccggggcaat gttttatttt gccgcagaat
       61 acggagcatg cctatggcag ctcggagcat gacccctgga cgatctactg gctgcatttt
      121 ggcggaaacg tgttgtccag cttcaatgcg atgcccatca tccagaaaca tttccggccg
      181 catgccgtcc gcagcagcaa tgaaatgatc acgctttttt cgaagatcta caagaccctg
      241 gagcttggct acagtatcga caacctgctt tttgcgaata tgtgtctctc gcattttatc
      301 accctgttca tctataacca gaaacatttt gccgcctcac cctccgaaaa gctcgactgc
      361 atcgacagcg cgatcctttt catgcaggaa catatcaacg agaacatcac gctcaatgat
      421 ctgagtcacc gctataatta ttcgccgtcg cgattttcca gtcttttcaa acagcggacc
      481 ggctatgcgc cgatagatta tttcatccag atgaagatgc aaaaggccag ccagcatctg
      541 gggattaccg ataaatcggt caaggacatc gccctgagca tggggttcga cgatccatat
      601 tatttttcca aacggtttac gaaagtgatg gggatgtcgc cgaccaagta ccggtccgtc
      661 acgagagatt gagctgtggc ctccggctca cggggcacgg ggctcacgag gaacggggca
      721 cccgggtccg cggccagcat gcgcgcgcct catttctttc gcggcgccgt tatatcccca
      781 ttgaaagtta taggatccct gttcagattg gtcacctgga gcgtcgtata tccggccgtc
      841 gaaatagtga gcgtcatcga ctgcacttca cggtgatctg tcggcttgat gacgatctcc
      901 cagcccccgc ttttccgcgt ggtggcggta tagtcgaatt tggtggaagt gaattgaagc
      961 gggctctgtg tcgggttcat aggggcgaca aaggcgcgac cgaaataggg cagctcgctg
     1021 acgacagccg tcggggtcac cttcagatcg tattcggtgg tcagatgacg gacgttgccg
     1081 cgcatgggaa gggcagtctg tgcctggaag ataaaatttt tggattcgac catatccttg
     1141 atggcggccg ctttttcatc ggcggattgg gcaaaggccc ggagggatag aagggctacc
     1201 cccgcaaata gaaataacct tttcataatc gttgaattat accataaatt tagacaattc
     1261 gaatgctctc atatatggct aacagcgaat ttgatgccgt agtggtgggc tcgggtccca
     1321 atgggctcgc ggctgccatt ttgcttcagc agcagggtct ttccgtcttg ctgatcgagg
     1381 caaagtccga catcggcgga gggttgcggt cggccgggct gaccctaccc ggtttcgtcc
     1441 acgatatttg cagttccgtc cacccgctgg ggatcgcctc cccctttttc agcagcctgc
     1501 ccctgaaaga tcacggcctc gaatgggtag aatccccgat tgccgccgcg catgcccttg
     1561 atgggggcag ggcggtatgt cttatgaggt ccgtggcggc gacggccaga ggtgttgggg
     1621 ccgacgagaa cagctatcgt cggctattgg acccgttggt ggaagattgg ccggagttga
     1681 tgccgcagat ccttgggcca ttgagatttc ctaagcatcc attgatgatg gcgcgttttg
     1741 gtttggaagg attgcgttcc gtcgcggcat tggcccgccg cttcgatacg atggaggccc
     1801 gcggattgtt ggcgggtatg gctgcgcatg ccatgcagcc tctgaccaat ctgaccacgg
     1861 cggccgtcgc gcttcttttt ctgctggcgg gtcatggaaa aggctggccg ttcccgaaag
     1921 ggggatcggc tcgtctggca aatgcgctgg cggattattt ccgaaagctg ggaggcgtca
     1981 ttcaaaccgg ctcgccggtc acggatctgg ggcagctgcc gccggctaag gcgttgttgc
     2041 tcgatctcac tccgagacag ctgttgcaac tgggcggtca tcgctggaca tcgctatatg
     2101 cgtggcaact ggagcggtac aggtatggta tgggggtctt caagatcgat tgggcgttgg
     2161 ccgaacctac cccattcccg gctatggatt gccggcaggc ggccaccgta catctcggga
     2221 atagcttcga agagatcgcc gcaggcgagg cgggcacgat caggggcgta catgccgaaa
     2281 aaccgttcgt tctttttaca cagacctctt tattcgaccc gtcccgtgct ccagccggca
     2341 aacaaacggc ctgggcctat tgtcacgtgc cgggagggtc gcggagggac atgaccgacg
     2401 ttatcgaatc gcaggtggag cgattcgcgc ccgggttccg cgatcgtatc ctcgcgcggc
     2461 atgtgatgga cagtgcgcag atagaatcct acaatcccaa ctatgtcggg ggcgatatca
     2521 atggcgggat catggatatc gggcagctgt tcacgcggcc ggtcctccgc cggagtccct
     2581 accgaacctc ggcgaagggc atctatatct gttcgtccgc cacgcccccc gggggcgggg
     2641 tccatggaat gtgcgggtat tatgcggcta aacgcgcctt acgggatgtg ttcggtatac
     2701 gctaagccct ggcgaaattg tcccgcccca gccgtccgcc ccacgggcat tcaagcctcg
     2761 gtcatcctca cccttctctt gcttttcctg tatttttcgg aaatgatctc gctcatcggg
     2821 cgttggtaga ccttgctttc gacccaggag acgaggtcca gataggcgaa ggcgcgggct
     2881 tcataccgat ttccctcgaa ccctttgatc ttttcgagga atttttcgaa ttcgttccgc
     2941 aggccggtgc gcgaggtttg aaaggaggtt cgaaggaacc gaaacatttc ttcctcgact
     3001 ttggtgaggc gttcgcgtcg ggccatgaat cggtatacgg atttggtcag cgactccatc
     3061 agatcgaaat tcttcagctc gtaatgcgcc atcaggtgca gcacgcgggc atagcattga
     3121 agatcgttgc ggaggtccgt cgggtcgttg attatccgtt gcagatagtc gatacaggtg
     3181 gcatgatcgg cggcgccgaa atacatcgtc gcgatcttgt agttgagaac gaggatgcgg
     3241 tggcggtcga tgaagaggtc gtattcggcg agcttttctt cgataccggg aaccatcgcc
     3301 agcccttcgg cgaaattgcc ctgcatgaat acctggttga tccgggcggt cgtgatatag
     3361 acgaatgact ggatccggaa attgtcgttt tcactgaccc tgtcggtctg ggaaaagttt
     3421 tcgaactgct gcaggatacg ggcgaagcgt tcgtggttgc gcagatcgaa atgggcgttg
     3481 agcaggttgt gaagcgcctt gatatagtga ccggtctcga cccggatcat caggggctgt
     3541 tcctcgaaaa gatcgagcca tttttgcgag tagcggtaat acatgaggaa atcctgccgg
     3601 ataaaggcgt accagttgtg gctctggtag agatagaggc gttcgtagaa gccggtctgt
     3661 tcccaggcgt tgggaggcag ctgttcgcga aggtactctc ttagtgcggc ctcgtctttt
     3721 tcattacggg aatgcccatt gtggatatac cagctataga ccagcagggc taagttggaa
     3781 agacgggcta ccatatcgat ccggctgatg acggatttgg actcggcgga caattgttcg
     3841 gcccgcatct gcatgctatg ggtgatatgc agtgtctcga tgcgtttttc aagcgccacc
     3901 acctggacga ggaagttgaa cttctggttg gctttggccg tttccttggc gcggtcgagc
     3961 atccggaggc tttgctggaa gagccctttt ttgtaaagga tatgggcgta atcgaacatt
     4021 tcgttcagct gcagatcgat gctgtcggcg cttttcagca gacgcaggct ggcgagtatt
     4081 tgcttataga gatgggtctt gaggttcgat agttgcggtt tttcgatccc cggcagcttt
     4141 ttcaacaggt tcttttcgtc gtaatcggtc agcttatcca tggcatcgaa cagttgtacg
     4201 atcttcaggt cctccttagt ggagctccgt ttgatgtaca gcttaaaatg tcttttttcc
     4261 gacttttcga gcgaatgtat aagctgaaac aaggtatctg aaaaccggtt ggccataatg
     4321 cgctaattag caacccctta tttgtgccgg gggctttctt tttgcaatct accaagaatt
     4381 cacatatttc gatctttcct tcccttttat ttcagggcgc tgcgatggtt aactggatac
     4441 aaaaattgta gttatgaaaa gagtacttcc tgtattctcg tttgttttag tcatataaag
     4501 tgtagtgttt tctcaatgtg acctttgagt cctccggggt ttgtgtctac aacgccctgc
     4561 tgtttaatgt gccgctggca agggctcctt cgcaagctgg cgcttgcctc cgtcggcttg
     4621 actgcgcgaa agaaaaatat actaattttt ttgaaagtat catgggaccc ggccgttata
     4681 aatgtaaaaa tgcccctggc tttttcgggt atactattcg ttcaaaatta actgctaaac
     4741 caagagtaga aagtgcaatc ttttttcttt attcgccgtg aaggccgata tgtaaaggta
     4801 gacttccgga atatcgtcta catcgaggcc aagaagaact atacccggct gatgctggag
     4861 gacaagtcca ccgtgctggt cctcggcact ctcaagcaat gggagagatt gttgccttgt
     4921 gacctgttct gcagggtcca tcggggttat atagtatcga tagagaagat cgtcgcattc
     4981 gataataagc atgtttatct gcccggtagt gagatcatca ccatcgggga tcaataccgc
     5041 aacgccttgc cggattcggt caccattttg gcccaggaac cggcgcattc gcctgcccgc
     5101 tcggtacgta aggacgttct gtccgaactt gaactttgac ccgcatattt cccccttttt
     5161 cctcgttatc cgatgggttt ttcgggtaaa accgcctgcc cggtggataa gaccgccgga
     5221 taggatcgcc ggataaaacc gcctgcccga tggataagac cgccggatcg gatcgccgga
     5281 taaaaccgct gcccgccgct tcccccgggg ccgttttttc gaccgcaagc ttgtcatcat
     5341 gaaaagtgct aaattggcag cgcggcggag aggcaaccgc tgggaaaaat aaggcgttta
     5401 gtctataaac tcattagaac acaggtaagt aagcagcata tcttggaaaa aatccttaat
     5461 ataatcgcag gctgtgcacg tgaagaccgg caaagccaga agatgttata cgaccaatat
     5521 tacggtctgt gtttgaagat agctttcaga tatgtcaaca cctatgagca ggcgacggaa
     5581 gtgacccatg acgcattttt gaagatgttt cgcaatttta gccgatttga ggttcgggat
     5641 aaggagaaag tggagatgtt gctgatcggc tggatcagaa gggtagttat caatgccgcc
     5701 atcgattaca tgcgccggga aatgaataac acccaaacct atccgatccc tgaacatgtg
     5761 tgggagcata aggacgacgg ccagtccagt gataatggcg tgctgtataa agaattgata
     5821 gcgctggtga aggatttgcc tcccgcctat cgtattgtgt tcaatctaca cgttatcgaa
     5881 ggttattcgc atccggagat agcaaagatg ctcgggatca cgacagggac gtccaaatct
     5941 aacctttcaa aggcccgggc gcatttgcaa aaagccttaa ccgcagaaaa aagtgaaaaa
     6001 atattatgtc aaatttaaag gacaccgagg ttgacgatct cattcggcgg gcgtccgata
     6061 aatacccgct gagaacagac agtgccgact ggattaaggt ggctgctgct ctggacatgg
     6121 atccggtccc tgccggaacc ggggatggcg atgacaaacg caggaagcgg agattcttct
     6181 ggctgctgct gttgttgccg ctgggcgggg caggttacta taccctgacc catacaggaa
     6241 atgtggcagg ccaagccggc catgccgcag tcgccgtggt cgccggtcag acgggccatc
     6301 ccgctgcggc cgtaacgact ggtcagacgg gccattctgc tgcggccata acgactggcc
     6361 agacgagccg gcaggatgtg ggtggaaccg cgaagaacgt cccgggcgca ggcctgcagg
     6421 acgtgggtag aaccgcgaag aacatcccgg gctcaaaagg cgtggatggg aaggcggttc
     6481 gtatggaccg ccaggtgaag gacgggacgc cagcgtcggg aaattccgtt gcagcggacc
     6541 ggcaattcaa caatggatcg gccggagcag tgaacccgaa tagagccccg gacccgggca
     6601 gagaccttgc taccgcccct caaccctata tcgccaccgc cccgctttca cttgaatggg
     6661 caggagatat gcaaagagct cacatcccgc atgactaccg tttgtcggtg aacgtgaccg
     6721 caccgccgcc ggcaaagaac accgatcctg cgaaaccgaa aaaacagaag accgcctatt
     6781 tgtacgccgg catcctcggg gcgccggata tcagtacggt caagatgcaa agcgtcaaag
     6841 gcatcggttc gaccttcgga gtactcgtcg ggtatgcgtt caatccacgc tggtccatcg
     6901 agaccggcgc ctacctcgac cggaaaaagt attatacgga aggagaatat ttcaataaaa
     6961 gcaatctgaa tctttacggc ggtacgatgc tgaatgcgga cggcacctgt tctatgtggg
     7021 agataccgct gaacgtgcgc tatacattca accccaacgg gaagacgaaa ttttttgcga
     7081 ccgcaggcgt ttccacctat ctgatgacga aggagaatta tacctatgcg taccagagcg
     7141 cgctcggctg gcagggtcaa aagacctaca ataacaaaaa cccctctaaa taccccttct
     7201 ccatcttcaa cctgagcttc gggttcgaac aaagacttgg aagggtgggg agtctacgac
     7261 tggaacctta tttacggctg cccctcgaag ggatcggcac gggcaaactg cccatcatga
     7321 gcgcgggcgt gaatatcggc attacgcgtc gcctatggta agaaagattt aacctgatca
     7381 tatcatgcga aaaaaacgga ccattatttg gataggagtt ctattgctgg tgctcgtcat
     7441 ggcgggatgg ggctggtatc tcttcgacaa accgcatcgg agcgcggcgg cgtactccgc
     7501 agacgtcacc atcgatgccg ataccctcta tcatcaatac aaggcggatg agcatgccgc
     7561 cgatctgaag tatgtgggaa aggtgctttc tgtggccggc aggctgacgg agatacagcg
     7621 cagcggaaat tcggagatct ggatcctgtc gatacaaccg ggcggcggag ggatcaattg
     7681 tgagctgttt gccgggacaa aggtcgatcc cgagcccaaa ccgggagatg ccgtcacgat
     7741 caagggacgc tgcacgggct ttctcatcac cgacgtcaac ctcgcggatt gcgtgcctga
     7801 caataagtga acgggaagaa gcgttccgga aaataaacca accctctatt aatattttat
     7861 gaaccgaaag tttttattgg ccaccctatg catcggcgcg atcgtcttct atctcgccgg
     7921 atgttctaaa gaaagcgcgg ataagctatc cgggagcgcc acctgcgata cggccagcgt
     7981 ctcatactcg acacagatcg tcgatatcct gcaaaataat tgctacagct gtcacagcgg
     8041 ggcgacgcca cctacgggca tccggctcga cacctatgcc aacctgaaag tatttgtcga
     8101 caacggcttc ctcgtcagtg cggtgacgca gaatggcgtc gttaccccca tgccgtacgg
     8161 cctgcccagg ttgcctgcct gcgaggtaaa taccatcgtc gcatgggttc atcagggggc
     8221 gccggccaat taaaacatct tttaaaaatc tactatgaag caactattca tcgccctgtt
     8281 cctatatctc gccttcttcc ggccctccac cgcctatagc cagatgtatt ccacacgcac
     8341 gggtttcatc ggattttatt ccaaaacggc gctcgaagac atcaaggcgg agaacaacca
     8401 ggtctatgcc atcatcgatg ccggcaaaaa gaacgtagcc ctggcggtgc tgctgaaagg
     8461 gttcatcttc acaaaagagc tgatgcagga acatttcaat gagaactatg tggagagtga
     8521 caaatacccc aaggctacct tcaccggcgc ctataccgga gccgtcggcg gggatggcgt
     8581 ctacaaggtc accatcaaag gcaacctgac gctgcacaac gtgacgaagc ctatcgaatc
     8641 agaagccacc atcgagataa agaacggtca cctgctcggc aagacggaat tcaaggtcag
     8701 acctgaagac ttcaatatca atataccctc tattgtccgc gacaagatcg ataaagaaat
     8761 aaccgtaaaa gtgaatttcg attgtagttc caaataaatc tatcgcaaca tgaaccccca
     8821 aagaatattt tttttgctgc tttccctcgc tgctgccacc ggcctccgcg cacaggacag
     8881 caccttgctg aatatgctca gcgattccat gtccgcgcac aaaggacgca gctatgtgac
     8941 gggcaccttc aaggccacgc atatcatcaa tacgcaaacc atcgagtcgc ctgcaaagaa
     9001 caatctcaac ttcgtcattc agcaccgttt tggaacgttg aacagcggcg cgtataattt
     9061 tttcgggttg gacaatgcca cgctgcgact ggggctggac tacggcatca ccgaccggct
     9121 ggcagtgggc atcggacgta gcagctatct gaagaccttc gacggctatc tgaaatacaa
     9181 gctgctgcgc cagaccgaga gtgccggcat gcctgtctcg gtgagccttc ttggagcgat
     9241 caccgattat acccaggatt tccccgaaga gacctatctg aatatgaagt accggacggc
     9301 ctatgcgggt gaattgctca tcgcccgaaa ggtgagcagc catctttcgt tgcaggtgac
     9361 gccgacctgg ctgcattata acctggtccc tacctcaaac gataaaaacg atgtgtttgc
     9421 cgtcggtctc ggaggccgga tgaagatcac gaggcggatg agcatcgatg ccgagtacga
     9481 tatagtacct tccggacagg tggtctctac gacgacgcac aactcatttt cgctgggatg
     9541 ggatatcgag accggcggtc acgtcttcca gctggtattc agcaattcgc aaagcatggt
     9601 ggaaagccag tacctgacac agaccaccgg cacctggggc aagggagata tctatttcgg
     9661 tttcaacata tcgaggaatt tcaatttgag taaaaagcgt tgatcagaaa aatactgttc
     9721 atacttactc cgggcgagcg acggcaagga gctttccttg cgtgtgggaa tgcttttatc
     9781 agcgtcctcg atatatgtgg tctgggagcc ctgctttatc ttgtggcgtt ttataccggg
     9841 cagggggtcg atacacgtct ttcctggctt cccgcatggc tcagggatag gaattcgcta
     9901 gcgcccattt ctgttttttc cctgcttttc ctggtcaaga gcgcaggagg atattggatg
     9961 ctgcagtcgc aatatcgtta tgtataccgg gtagcctcgc gtatatcgga aagcaacctc
    10021 ctgcgttacc tggaagggtc gtataccgac tatgtaaggg tggatgcctc ggtgcagatc
    10081 cgaaggataa gccaacagcc gatcgaattc tgccagtata tattggcggg cctccagcaa
    10141 ctggtgaccg aaggctcgct ggttgcgctg tccatcgcgg gcatcgtctg gtataacagc
    10201 aggcttttcc tgttgctgtt atgtctgctg gtgccggccg taagcatcct ggcctctttt
    10261 tccaggcggc ggctgaagtc cgtaaggaga gacatcaaga gcagcgggga aaaaagtctg
    10321 caacaccttc gggaagcgct cggcggttac gtggaaggca atctttacga tagcatttca
    10381 ttcttcgcgc gacggtatgg acggcgacag gccgatttga acgaacacct gtccgttctg
    10441 cagatactgc aaggcatccc gtcccggctg atagaggcct ttgccgtatt cgggctcttc
    10501 cttctcattg tgtttgcccg atggacgggt aacgcccagg ccgcgtcagc tattacactc
    10561 ggcgcctttc ttgcagcggc gtacaagatc atccccgggc tggtacggat gttcaatatc
    10621 accggtcaga tcatgaccta ccggtttacc gtggaagacc ttgtgacgga ggcccggaaa
    10681 ccacaacgca tgtccgttcc cgcggtccct tctatccgcc agatcaccct tcgaaatgtg
    10741 gaattctcac agccgcaggg ccgcgtggtg aaaggactga acctgtgt
//
LOCUS       DASYSV010000440         5064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_987580, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000440 DASYSV010000000
VERSION     DASYSV010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987580"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VGQ51_13540"
     CDS             <1..438
                     /locus_tag="VGQ51_13540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV9037650.1"
                     /translation="QFIPGSVYNEKDGVLETLMTVNLYSAYYLTRQLLPSMMTRRSGH
                     IFNICSVASLQAYPNGGAYSISKYALAGFSANLREEMKSYGIKVTTVYPGAAYTDSWA
                     GSGVDPHRIMEAEDVAAMVYAASRLSPQATVEEILLRPQLGDL"
     gene            523..2529
                     /locus_tag="VGQ51_13545"
     CDS             523..2529
                     /locus_tag="VGQ51_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEV9037651.1"
                     /translation="MEWVSPIKSPVIANILRLTFIIGISLLPALCVSGQVKFTTVVSS
                     QDVGKSDYFQVEFVVENAKQIDDLTPPDFPGFRIAQGPIQSSGMSIVNGNMSQYKGLS
                     FVLQPLKTGKFTIGGAVATVDGKQMRSNAVVINVHPGSSPSANAGPPAGGNPFAASPF
                     AQSPYADPFGPDPGEVDRDYVLRQGENVKDKIRKNMFVKVQVSKNSCYVGEPIVATYK
                     LYTRLISESRVTKRPSLNGFSVYDMVDPSLDAVSVEKLNGKNFTVHTIRKTQLIPLQA
                     GNIDLDPVELENTVHFVKEGGRPENRHSGNSIRDLLDQMADEANFGPSVDENVTLDTK
                     PVTITVKPLPEVNKPAGFNGAVGSFSVEATLANRILSAQDEATLHVVVRGKGNLPVVA
                     APVVNWPAGIEAFDPTAKEEVNKTVVPMSGSKSFDYIFIPKSAGHYTIPAVNFPYFDP
                     ATQTYKTAGTSPIVINVAASAKKDKQPATGVTRQAQAVQPEGIVPMLRSHLEWIAVFL
                     LFAGVAVYFWLQNLRLKKKEATLRTAPVTVPERPVSPANRDRSANIPVITGASYFSAT
                     QVTDPLAEARQFFENGDSKGFYREVNRAVWKAMARKLDLPASQLNKPNSLYQLELKGW
                     DSTSLLTLDSLLNECEMNLYTPEYDRWNMQQLLRQAESVLEKLA"
     gene            complement(2536..3231)
                     /locus_tag="VGQ51_13550"
     CDS             complement(2536..3231)
                     /locus_tag="VGQ51_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9037652.1"
                     /translation="MKKSILLVEDEENLHEALKLNLELEGYEITSAFDGTQALKAVQA
                     EYFDLIILDVMLPGMDGVTVTETIRVQHNEVPILILSAKNASADRVLGLKKGADDYLT
                     KPFNLEELLLRIHKLIEKNEKMQEKGDIGDVYSFGRNSIDFKAQEAITKDGQKIQLSR
                     KEAMLLKLLIENRNEVVTREKILQTVWGYNVYPTTRTIDNFVLNFRKYFEEDSRHPKY
                     FHSVRGVGYKYTE"
     gene            complement(3320..4294)
                     /locus_tag="VGQ51_13555"
     CDS             complement(3320..4294)
                     /locus_tag="VGQ51_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV9037653.1"
                     /translation="MLFKKKRLEVAITVYWILLAYIVTGMVYWFIALEARNEQITTFR
                     LHELDRADPQYVTRVKDIHREKERRMAQYVGEGSTFLGLILLGAIFVYREVRRQIRSQ
                     LQQQNFMMAVTHELKTPIAVTKLNLETLRKHKLDEQRQQKIIQAALQETDRLDTLASN
                     ILVASQLEGGGYLQSKELTDLSDLLRHSMQEFRHRVPERQWEEKIEPDCRIMGDALLL
                     QLLISNLVENALKYSPREAPITVMLGRKGSHVALSVIDRGPGIADDEKKKIFGKFYRT
                     GQEETRRTKGTGLGLYLCRKIAEDHKATLKVSDNSPAGSIFTVTFKAR"
     gene            complement(4305..4784)
                     /locus_tag="VGQ51_13560"
     CDS             complement(4305..4784)
                     /locus_tag="VGQ51_13560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HEV9037654.1"
                     /translation="MKNIAFVLLFVCCGTWLSCTSPTGVFEKDIALPHQQWESSFRPT
                     FTFDISDMDTAWRYNVYIVLRHTDAYNFNNIWIRGTVKMPGDSAARSDRYDLPLATNE
                     KGWLGSGMDDIFEQRVWVQQSTKFDHPGTYSFTLEQIMREDPLKHVLNVGVRIERVL"
     gene            complement(4915..>5064)
                     /locus_tag="VGQ51_13565"
     CDS             complement(4915..>5064)
                     /locus_tag="VGQ51_13565"
                     /inference="COORDINATES: protein motif:HMM:NF019940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HEV9037655.1"
                     /translation="GKKLSFILQEIGREINTTGSKAYDSTIQQCVVLMKDELEKAKEQ
                     VLNVL"
BASE COUNT         1117 a         1389 c         1313 g         1245 t
ORIGIN      
        1 caattcatcc ccggaagtgt atataatgaa aaggacgggg tgctggagac gctgatgacc
       61 gtcaaccttt acagcgctta ttacctgacc cggcagcttt tgccatcgat gatgacgcga
      121 agatcggggc atatcttcaa tatctgctcg gtcgcatcgc tacaggccta tcccaatggc
      181 ggcgcctata gtatcagcaa atacgctctg gccgggttct cggccaatct ccgggaagaa
      241 atgaagtctt acggcatcaa ggtgacgacg gtataccccg gcgccgccta taccgacagc
      301 tgggcaggct cgggtgtcga ccctcaccgc atcatggagg cggaggatgt agccgcgatg
      361 gtgtacgccg ccagccggct ttcgccgcag gcgaccgtgg aagaaatact gctccggccc
      421 cagctgggcg atctttaacg tgccggaggc accttgtccg cacattcccc cgcatcttta
      481 agaaagtttt aacccttcga tttttatatt tgcataggca ctatggaatg ggtcagccct
      541 ataaaatcac ccgttatcgc taatatttta cgtcttacgt tcattatcgg gataagcttg
      601 ttgccggcac tctgtgtctc cggccaggtg aagtttacta cggtcgtcag tagccaggat
      661 gtcggcaagt ccgactattt ccaggtggaa ttcgtcgtcg agaatgccaa acagatcgac
      721 gatctcaccc ctcccgattt tcccggtttt cgtattgccc agggtccgat acagtccagc
      781 gggatgagca tcgtcaatgg caatatgtcc caatacaaag ggctgtcctt cgtgctacag
      841 ccattaaaaa cgggaaagtt caccatcggg ggagctgtcg ccaccgtgga cggcaaacaa
      901 atgcggtcca atgccgtcgt catcaatgtt caccccggat cttctccttc cgcgaatgcc
      961 ggccccccgg cgggtgggaa cccttttgcc gccagccctt ttgctcaatc tccttacgcg
     1021 gaccctttcg gtcccgatcc gggcgaggtc gatcgggatt atgtgctcag acagggtgag
     1081 aatgtcaagg ataagatcag gaagaatatg ttcgtgaagg tgcaggtcag caagaattcc
     1141 tgttatgtcg gggaacccat cgtcgctacc tataagcttt atacccggct gatctccgaa
     1201 tcgagggtaa ccaaacgtcc cagcctcaac ggattcagcg tttatgatat ggtggatcca
     1261 agcctcgatg ccgtgtcagt ggaaaagctg aatggcaaga attttaccgt gcacaccatc
     1321 cggaagaccc agctgatccc gctgcaggcg ggtaacatcg atcttgaccc ggtcgagctg
     1381 gagaataccg ttcattttgt aaaagaagga ggacgacccg aaaaccgtca tagcggtaac
     1441 agcatccgcg atcttctcga ccagatggcc gatgaagcca attttgggcc atccgtcgac
     1501 gaaaatgtca cgctcgatac caaaccggtg accatcacgg ttaaacctct gccggaagtg
     1561 aataagccgg caggtttcaa tggcgcggta ggcagtttct ccgtggaagc cactctggcg
     1621 aacaggatcc tgtctgcaca ggatgaggcc accttacatg tggtggtacg cggcaagggc
     1681 aatcttccgg tagtcgccgc gcccgtcgtc aactggcccg cgggcatcga ggcattcgat
     1741 ccgacggcca aagaagaggt gaataagacc gttgtcccca tgagcggctc caaatccttc
     1801 gactatattt ttatcccgaa aagcgcggga cattatacaa taccggctgt taactttccc
     1861 tatttcgatc cggccacgca gacctacaag actgccggta cttcgccgat cgtcatcaat
     1921 gtggccgctt ccgccaaaaa agacaagcaa cctgcgacgg gcgtgacccg ccaggcgcag
     1981 gcggttcagc ctgaggggat cgtaccgatg ttacgcagcc atctggaatg gatagcggta
     2041 ttcctgctgt ttgcgggtgt agccgtttat ttctggcttc agaatctccg gttgaagaag
     2101 aaagaggcga cgctgagaac agcgcccgtc acggtgccgg aacgacccgt atcgcctgcg
     2161 aacagggaca ggtccgccaa tatccccgtt attaccgggg cttcttattt ttccgcaacg
     2221 caggtcaccg atccgctggc ggaggcccgt cagttcttcg aaaatggcga ctccaaaggt
     2281 ttttacaggg aagtcaaccg cgccgtctgg aaggcgatgg ccaggaagct cgatctcccc
     2341 gccagtcagc ttaataaacc aaacagtctc tatcaactgg aactaaaggg ctgggacagc
     2401 acttcgctgc ttaccctcga tagtctgctc aatgaatgcg agatgaatct gtatacgccg
     2461 gaatacgacc ggtggaatat gcagcagttg ctccggcagg cggagtcggt acttgagaaa
     2521 cttgcttagc caccattact ccgtatactt atatcctacc ccgcgcaccg aatgaaaata
     2581 tttcgggtgc cggctatcct cttcaaaata tttccggaag ttgagtacga aattatcgat
     2641 ggtgcgggtg gtcggataga cattgtatcc ccacaccgtc tggaggatct tctcacgggt
     2701 gacaacttca ttgcggtttt cgatcagcag cttcagcaac atcgcctcct tcctgctcag
     2761 ctggatcttt tggccgtcct tagtgatcgc ctcctgcgcc ttgaaatcga tgctgttcct
     2821 tccgaagctg taaacatctc cgatatctcc tttttcctgc attttttcgt tcttctcgat
     2881 cagcttgtgt atcctgagca acagctcctc gaggttgaag ggtttggtca gatagtcgtc
     2941 agctcccttt ttcagaccga ggacgcggtc ggcgctggca tttttggcgc tgaggatcag
     3001 gatcgggact tcattgtgtt gcacccggat ggtctcggtg accgtgacgc catccatgcc
     3061 gggcaacatg acgtcgagga tgatcagatc gaaatattct gcctgcacgg ccttcagcgc
     3121 ctgtgttcca tcgaaggccg aggtgatctc atagccctcc agctcgagat tcagcttgag
     3181 cgcttcatgc aggttctctt cgtcctctac cagcagaatg gattttttca taaattgttg
     3241 tttgcacttc gccggcatgg ccgcgaccgc tactgttacc gcgctttgat gcctgatgat
     3301 tgcccttcgg gccaaagcgc tacctggctt tgaaggtcac cgtaaaaata cttcccgccg
     3361 gcgaattatc gctcactttc agcgtagcct tatggtcttc ggctattttg cggcaaagat
     3421 agagaccgag acccgttcct ttggtccggc gggtctcttc ctggcctgtg cgatagaatt
     3481 tcccaaagat cttctttttc tcatcatcgg ctatccccgg tccccgatcg atgacggata
     3541 aagccacatg gctacccttt ctgcccagca tcaccgttat cggcgcctcc ctgggagaat
     3601 atttgagggc attctccacc aggttactta tcaacagctg caggagcagc gcatccccca
     3661 tgatcctgca atccggttcg atcttttctt cccactgcct ctcaggcacg cggtgacgga
     3721 attcctgcat cgaatgtcga aggagatctg aaagatcggt tagctctttg gattgcagat
     3781 accctcctcc ttccagctga gaggcgacca ggatgttgct ggccagcgta tcgagccggt
     3841 cggtctcctg taatgccgcc tggatgatct tttgctgtcg ctgttcgtcc aatttgtgtt
     3901 tgcggagggt ttccaggttg agctttgtga cggcgatagg tgttttcagt tcgtgcgtga
     3961 cggccatcat gaaattctgc tgttgcaact gcgaacggat ctgccgtctc acttcccggt
     4021 aaacgaagat cgcccctagg aggatcagcc ctaaaaaagt gctgccttct ccgacatatt
     4081 gcgccatccg tctttctttt tcgcgatgga tatcctttac ccgggtgaca tactggggat
     4141 cggcccggtc cagctcgtgc aaccggaagg tggtgatctg ttcgttcctc gcctcgagcg
     4201 caatgaacca atagaccatg cccgtgacaa tatacgccag cagtatccag tagaccgtga
     4261 tcgctacctc caggcgtttt ttcttgaata gcatagtgac ccgtttacaa tacccgctcg
     4321 atccggacgc cgacgttcag aacgtgtttg agggggtcct cgcgcatgat ctgctccagc
     4381 gtgaaggaat aagtgccggg gtggtcgaat ttggtggatt gctgcaccca gacgcgttgc
     4441 tcgaagatat catccatccc gctgcccagc catccctttt cattggtggc caggggaaga
     4501 tcgtagcggt cgctgcgggc agccgaatca cccggcatct tcaccgtacc cctgatccag
     4561 atattgttga agttataggc atccgtgtga cgcaggacga tgtacacatt gtagcgccag
     4621 gcggtgtcca tgtcactgat atcgaaggtg aaagtaggac ggaaactgct ttcccattgc
     4681 tgatgcggca atgcgatgtc cttttcaaaa acgccggtag gtgaagtaca ggacagccag
     4741 gttccgcagc aaacaaaaag gaggacgaaa gcgatatttt tcaatgaatg ggatttatgc
     4801 aaaaataaac gcatttttcg caactggaaa tgccggaggc atttcgtccg aaggaccgac
     4861 attagccggg tgcgaaaaat gcggtacatt ttccttaaac gcatttttcg cactttataa
     4921 tacattcaga acctgttcct tcgctttttc cagctcatcc ttcatcagga ccacgcattg
     4981 ctggatggtg gaatcgtagg ccttggatcc ggtggtgttg atctcccggc ctatttcctg
     5041 taagatgaaa gagagcttct tccc
//
LOCUS       DASYSV010000441         8118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2267606, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000441 DASYSV010000000
VERSION     DASYSV010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267606"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1604)
                     /locus_tag="VGQ51_13570"
     CDS             complement(84..1604)
                     /locus_tag="VGQ51_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV9037656.1"
                     /translation="MKSKVAILGACAVIAGGVLFANGKVRSAIGGDQAYNSILDTTHA
                     PAFRTAYNTPAAVQPVDFEKAASAAVPSVVHIKVNIKANHIAGREGPDHDPFGGQFGG
                     DDFFRRFFGDGNGHAMPQPDQKASGSGVIISSDGYIVTNNHVVNGATSVTVTLNNRTN
                     YTAKVIGTDPNTDLALIKIDGKNLPVMTVGNSDDVKLGQWVMAIGYPLNLDVTVTQGI
                     VSAKSRNIGINTGASAPVEAFIQTDAAVNPGSSGGALVNTNGELIAINSAIASPTGSF
                     AGYAYAIPSNLMKKVIGDIMKYGSVQRGYLGISMAPEGLDDSKKKELGINSDVAGVFV
                     MEVSPNSAADQAGLKKGDVITEVNGQPVTSDARLAEIVATQKPGDHVKLDYVRNGEQK
                     TADAVLQAKLGAFASQKSAAVESLGADFSNLSKDDATKLGVDGGVVVNNIRNGVLSNQ
                     TNMRPGFIITKIGDIKVTTVDQLKDALSKQGSNFQIEGVYPDSNEVYYYGINDFRK"
     gene            complement(1861..2319)
                     /locus_tag="VGQ51_13575"
     CDS             complement(1861..2319)
                     /locus_tag="VGQ51_13575"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEV9037657.1"
                     /translation="MPIDEKLADRIRERFADLPHVEEKAMMGGLTFMYNDKMCVGIFR
                     GDLMCRIDPEVYEEALEKQGCHAMEFKGRVMPGFVLIDATGRHTKKQFDYWIDLALDF
                     NKKAKSSKSSKSGKPSRPSKPTVPSTPTKPSKPTTSSTPSKPSKPSKKRR"
     gene            complement(2340..3833)
                     /locus_tag="VGQ51_13580"
     CDS             complement(2340..3833)
                     /locus_tag="VGQ51_13580"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 39"
                     /protein_id="HEV9037658.1"
                     /translation="MKTRYLLPLILIAFSAHAQNQAPDTVNVQWQRTQIISKTTLTLQ
                     VVVNPPLRRGSAMHDSIFTALQSLGCDYVRYVPWLPYPRLGVAELRQPTPQRTFWDFS
                     VIDPMTLDFLNATKGHPVILNFSTIPAWMYKTSKPVTFPDDPYAVFWDYTQGNTPRDT
                     TYREIADYYARLYSWYTKGGFTDELGKRHESGYHYPVPYWEVLNEVDFEHGNTPESYT
                     RLYDAVTAAIKKESPGTRFVGMALADPNRPEWFEYFLNPKHHKGGTPLDLISYHFYAT
                     GGVQQGMDVLQYTFWDRADGFLNTTRFIDNIRKRLSPGTKTTIDEVGSILDNDNEVPD
                     RKTPIPNTYWNLSGATYAYVYLGLARIGIDIVGESQLVGYPTQYPSVSMIDWKNGRPN
                     ARYWVLRLLKDNFGPGDKFVQSGLAGTEPEAGDALTQQAIVTSHGRRLLLINRRNKTI
                     RLVMPKEMIGTTMQVVDPSTGDEAPAVIKVTETPVGLKPFAVAVVGG"
     gene            3954..5369
                     /locus_tag="VGQ51_13585"
     CDS             3954..5369
                     /locus_tag="VGQ51_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose isomerase"
                     /protein_id="HEV9037659.1"
                     /translation="MKIGLFAIGLDTYWPQFEGLEDRLLGYLRIVEGKLMAFHPGIVN
                     AGMVDTVDKAFAAGKLFRSEDVELIFLYASTYALSSTVLPVVQLAGVPVVILNLSSSS
                     SMDYVRINALGDRTKMTGEWLAHCSACPVPEIVNVLNRVGIRSYQVTGILEGDESCWA
                     EIGEWVAAARVKHVMRHNRLGLMGHYYSGMVDVYTDLTQQYACFGGHIELLEVEELVA
                     FRKAVGRGAALDRVQLFREVFEVLPECSALDLEQAAMTSLAMEGLVARYKLGSMAYYY
                     KGTGNPDNEEAIASIIPGNSWLTANGVPVACEYEVKNVQAMKILDSLGSGGSFTEYYA
                     MDFDADVVLMGHDGPGHIAIAEGKTIVRPLEVYHGKVGRGLSVEMSVKHGPVTLLSVV
                     EKRGGGLFLLVAEGETVDGPILQIGNTNSRYRFSGGARGFVNRWNAYGPAHHCAVGVG
                     HLSSCLEKLAALINIEMVKLC"
     gene            5397..6281
                     /locus_tag="VGQ51_13590"
     CDS             5397..6281
                     /locus_tag="VGQ51_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9037660.1"
                     /translation="MINYYRYLPISKEDQSWGLSVLNAGATRIAAGQEYPYRQHPSKY
                     YFCWSKGRVLDEYQLIYIAGGEGFFESSSVPECVVHAGTVLLLFPGEWHRFRPNMQTG
                     WDEYWVGFTGEVADNLMRGKFFEPPGAVLSIGYNAEIVALFSGIIEQTKTERPGYQPF
                     VSGMLLHLLGCVYSLSRQQSFSNADGVKSKMEQAIMLLRSKIDEAIDIHEIAEELNVS
                     YAWFRKWFKLYTGLAPQQYVIQLKMEKAKVLLADSGNSIKGIAYQLRFEHPLYFSKLF
                     KEKVGVSPDLYRKQCGGS"
     gene            complement(6278..6478)
                     /locus_tag="VGQ51_13595"
     CDS             complement(6278..6478)
                     /locus_tag="VGQ51_13595"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF019610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV9037661.1"
                     /translation="MLTHTHAPSLYRLRTVVLHELGHNLGLPHCPNPHCIMNDANEKI
                     ATIDNSRDRFCASCQSRLTAIP"
     gene            complement(6472..7296)
                     /locus_tag="VGQ51_13600"
     CDS             complement(6472..7296)
                     /locus_tag="VGQ51_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9037662.1"
                     /translation="MPVLLKSVLLFFLPLAVLTTHAQQKRSDTLIVHFAFDRSDLGPQ
                     DLDLLHQIARLSPDSVSIIGHTDIKGSVAYNQQLSIRRARSTQATLQNILSSNLPSIR
                     FEGRGKSEPLPGDDSLSRRAVVVVYYHDKPAPPVARIDTPPRVRSPDEPDTVISLNNI
                     NFIANTPVLTDAAREAMPSTISSLRSFSDRILEIDGFCNAPGPPLPPHDPLFKLSVQR
                     AKYIYDMLINSGFDSTRLRYKGLGNARPVNAHPTTTAEMDQNMRVEIKVFSKLPPS"
     gene            complement(7328..>8118)
                     /locus_tag="VGQ51_13605"
     CDS             complement(7328..>8118)
                     /locus_tag="VGQ51_13605"
                     /inference="COORDINATES: protein motif:HMM:NF023384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3472 domain-containing protein"
                     /protein_id="HEV9037663.1"
                     /translation="SVGYFGMQVNSATERRILFSVWSPFTTDNPSAIPDSLKVRLVKK
                     GQDVHTGSFGNEGSGGQSYLVYPWVAGRTYRFLLRAQPLSSAAGPAAPAAPASTPASA
                     RAATPVVTPVATPATSTPTATPTTASSITRFTAWFYAPEQAHWLLIASWDRPATHTWL
                     QHLHSFLENFEPDYGDQGRRVLFSNQWTRDSAGHWTPITRARFTGDNTARKGYRLDYA
                     GGSTTDGFWLRNGGFIQTPTPLDTWFTRPVSRQSPPDVDFAALP"
BASE COUNT         1707 a         1803 c         2616 g         1992 t
ORIGIN      
        1 ggaatgaggt cgctaaaaaa taaggccgcc tcccattttg gggggcggcc gacctttaat
       61 agagagaaca aaacaaacct tctttatttc ctgaagtcat tgataccgta gtagtagacc
      121 tcattgctgt cggggtagac gccttcgatc tggaagttgc tgccttgttt gctcagggca
      181 tctttcagct gatcgacggt agtcaccttt atatccccaa ttttcgtgat gatgaaaccg
      241 gggcgcatgt tggtctgatt gctcaggacg ccgttgcgga tgttgttcac cactacgccg
      301 ccgtcgacac ccagtttggt ggcgtcatcc ttgctaaggt tggagaagtc ggcgccgagg
      361 gattcgacgg ccgcgctctt ctggcttgcg aaggcgccca gcttggcctg aaggacggca
      421 tcggccgtct tttgttcgcc attgcggaca taatcgagct tcacatgatc gccgggtttt
      481 tgcgtggcga cgatctcggc cagacgtgca tcggaagtga cgggctgacc gttaacttcg
      541 gtgatgacat cgcctttttt cagaccggcc tgatcggctg cgctgttagg agaaacttcc
      601 atgacgaata cgccggcaac atcgctgttg atacccaatt cttttttctt ggaatcatcg
      661 agaccttcgg gtgccatgct gataccgagg tagcctcttt gtacggagcc atatttcatg
      721 atgtcgccga tgaccttctt cattaaattc gaggggatgg cataggcgta ccccgcaaag
      781 ctaccggtgg gagaggcgat ggcggagttg atggcgatga gctcgccatt ggtattgact
      841 agtgcgccgc cgctgctacc gggattgact gcggcatccg tctggataaa ggcttcaaca
      901 ggcgcggatg cacctgtatt gatgccgatg ttcctggatt tcgcgctgac gataccctgt
      961 gtgacggtta cgtcgaggtt gaggggatag ccgatggcca ttacccattg tccgagcttg
     1021 acgtcatcgg agttaccgac cgtcatgaca gggaggttct tcccatcgat cttgatcaat
     1081 gccagatcag tgttggggtc ggtgccgatg accttggccg tatagtttgt acggttgttg
     1141 agggtcacgg tgacggaggt ggctccattg accacgtggt tattggtgac gatgtaaccg
     1201 tcggagctga tgatcactcc cgaaccggag gccttctgat cgggttgcgg catggcatga
     1261 ccattgccgt cgccaaagaa tcgtctgaag aaatcgtcgc ctccgaattg accgccgaac
     1321 ggatcgtgat cggggccttc tctgccggcg atatggttgg cttttatatt tactttaata
     1381 tgtacgacgc taggtacggc ggccgaagct gctttttcga agtccaccgg ctggactgca
     1441 gcaggagtat tgtacgcagt acggaaagcc ggggcatggg ttgtatccag gatgctgttg
     1501 tacgcctgat ccccgcctat tgcagaccta actttcccat tggcgaagag gacgccaccc
     1561 gcgatgactg cacacgcacc gagaatggct actttacttt tcattttcat ggcttaattg
     1621 cttttagaat ttgtttgact gatgcaatga taacgggtgt gcaggagaat cgttttcgat
     1681 ggtgtgttaa ttgaatctta aagccatttt tgggctggaa gccacgctgg gcaagggttt
     1741 gggaggagtt aaagaaatga taattaagat tgggttgacg ccggatgtgc gaagtgtttc
     1801 gtatattcgt tggttgaata attttgtttg gggcttgccg cgacctcggg agacgttggg
     1861 ctatctcctt tttttggagg gtttggaggg tttggagggg gtagaggacg tcgtgggttt
     1921 ggagggcttc gtcggggtgg aggggaccgt gggtttggag ggcctggagg gtttgccgga
     1981 tttggaagat tttgaggatt tcgctttttt gttgaagtcg agtgcgaggt cgatccagta
     2041 atcgaattgt tttttggtgt ggcggccggt ggcatcgatg agaacgaagc cgggcatgac
     2101 gcggcctttg aattccatcg cgtggcagcc ttgtttttcc agcgcctctt catagacttc
     2161 cggatcgatg cggcacatca gatcgccgcg gaagatgccg acgcacattt tatcgttata
     2221 catgaatgtc aggccaccca tcatggcctt ttcttcgacg tgagggagat cggcgaagcg
     2281 ttcgcggatc cggtcggcta atttttcatc gattggcata gacacatttt ttttggtgct
     2341 catccgccga caaccgcgac ggcgaaaggt tttaacccga caggcgtctc agtgactttt
     2401 attaccgcag gtgcttcatc gccggtggac ggatcgacca cctgcatggt agtgccgatc
     2461 atttctttgg gcattaccag acggatcgtc ttatttcgcc ggttgatgag caatagtcgt
     2521 cgcccatgcg aagttacgat tgcctgttgg gttagcgcgt cgcctgcttc gggctcggtg
     2581 ccggccagcc cgctttggac gaatttgtcg cccggcccga aattatcttt taacaaccgg
     2641 agcacccaat aacgagcatt gggccggccg tttttccaat cgatcatgct tacgctggga
     2701 tattgggtgg gatagccgac cagttgcgat tcgccgacga tgtcgatacc tatacgtgcg
     2761 aggccgagat agacataggc atacgtcgca cccgacagat tccaatacgt gttgggtatg
     2821 ggcgtttttc tatccggcac ttcgttatcg ttatccagta tgctcccgac ttcatcgatg
     2881 gtcgtcttcg tgcccgggga gagtctcttg cggatgttgt cgatgaacct ggtggtattg
     2941 aggaagccat cggcccggtc ccaaaaggta tactgcagca catccatacc ctgttgcacg
     3001 cctccggtcg catagaaatg ataagatatc aaatccagcg gcgtgcctcc cttatgatgt
     3061 ttgggattga ggaaatactc gaaccattcg gggcggttgg gatcggcgag ggccatcccc
     3121 acaaaccggg tgccgggcga ttccttcttt attgcagcgg tgacggcgtc atataacctg
     3181 gtatagcttt ccggcgtgtt gccatgttca aaatccactt cattcaacac ctcccaataa
     3241 ggcacagggt aatgataacc cgactcgtgc cgtttgccga gctcatcggt gaatccgcct
     3301 ttggtatacc agctatataa ccgtgcatag taatcggcga tctcacggta agtggtatcg
     3361 cgcggggtat tgccctgggt ataatcccaa aagacggcgt aggggtcatc gggaaaggta
     3421 acgggcttgc tggtcttata catccatgcg gggatcgtgc tgaagttcag gatcaccgga
     3481 tggcctttgg tcgcattcag gaagtcgagg gtcatgggat cgatgacact gaaatcccaa
     3541 aacgtccttt gaggggtggg ctgtcgcaac tcggccacac ccaatctggg ataaggcagc
     3601 cagggcacat aacggacata gtcgcagccc agcgactgaa gggccgtaaa gatggagtcg
     3661 tgcatggcag agccgcggcg cagcggtgga ttgacgacga cctgcagggt cagtgtcgtt
     3721 ttcgatatga tctgagtccg ttgccattgg acgttcaccg tgtccggtgc ctgattttgc
     3781 gcgtgggcgg aaaaagcgat gaggatcagc gggagcaggt aacgtgtctt catgggataa
     3841 agttcgtcaa cgggatcgca tctccaaatg catcacgtgg aaatcgcaaa aagggaatca
     3901 acaatctcaa ataatacatc tgcgatccat atttgggtaa tagatttatg accatgaaga
     3961 tcggtttatt tgccattggg ctggatacct attggccgca gttcgaaggg ttggaagaca
     4021 gactcctggg ttatttgagg atcgtcgagg gaaaattgat ggcgtttcac ccgggcattg
     4081 tcaacgccgg aatggtagac accgtggaca aggcctttgc ggcgggcaag cttttcagat
     4141 cggaggatgt cgagctgatc tttttgtatg cttccacgta tgcgctttca tcgacggtgc
     4201 ttccggtggt tcagttggcg ggggtaccgg tggtcattct gaatttgtcg tcttcttcgt
     4261 cgatggatta tgtccgcatt aatgcactgg gagaccgaac gaagatgacg ggtgagtggt
     4321 tggcccattg ctcggcttgc ccggtgcccg agatcgtgaa tgtgctgaat cgtgtaggaa
     4381 tccggtcgta ccaggtgacc gggatactgg aaggtgatga gtcgtgctgg gcggagatcg
     4441 gcgagtgggt ggcggcggca agggtgaaac atgtcatgcg tcataaccgt ctgggtctga
     4501 tggggcatta ttatagcggc atggtagatg tatataccga tctgactcag caatatgcct
     4561 gtttcggcgg gcatatagag ttgctcgagg tggaggaact ggtggctttt cgtaaggctg
     4621 tcgggcgtgg ggctgcgctt gatcgcgtgc aattatttcg cgaggtgttc gaagtactac
     4681 cggagtgttc tgcattggat ctggaacagg cggcaatgac gtcactggcg atggaggggc
     4741 tggttgcccg ctataaattg ggttcgatgg cttattatta caagggcacg ggtaacccgg
     4801 acaacgagga ggcgatcgca tccatcattc cgggtaattc ctggctgacg gcaaacggcg
     4861 taccggtggc ctgtgaatat gaggtaaaga atgttcaggc gatgaagatc ctggacagtc
     4921 tcggctcggg gggctcattt acggagtatt acgcgatgga ttttgatgcc gatgtggtgt
     4981 taatggggca tgacggcccg gggcatattg ccattgcaga aggtaagacc atcgtccggc
     5041 cgctggaggt gtatcatggg aaggtgggca ggggtctttc tgtagagatg tcggtgaagc
     5101 acggtcccgt gacgttgttg tcagtggtag aaaaaagagg aggtggcctg ttcctgctcg
     5161 tggcagaggg tgaaacggtg gatgggccga tccttcagat tggaaacacc aatagccggt
     5221 accggttctc tggcggagca cggggttttg tcaaccgctg gaatgcttac ggcccggcac
     5281 atcattgtgc cgtcggggtc ggtcatcttt cttcgtgtct tgaaaaactt gcggcgttga
     5341 tcaatataga aatggtgaaa ctgtgttaga tttagggagt aaacgtgggt cagtctatga
     5401 tcaactacta ccgatatctg ccgataagca aggaggatca aagttggggt ttgtcggttc
     5461 ttaatgccgg cgccacccgg atagcagcgg gtcaggaata tccttaccgg caacatccga
     5521 gcaagtatta cttctgctgg agcaagggga gggtattaga tgaataccaa ctaatctata
     5581 tcgccggtgg cgagggtttt tttgaatcat cctccgtccc ggaatgcgtt gtccatgcgg
     5641 gaacggtact gttgcttttt ccgggggaat ggcatcgttt cagacccaac atgcagacag
     5701 gttgggacga atattgggta ggattcacgg gtgaagtggc ggataacctt atgcggggaa
     5761 aattctttga gccgccgggc gctgtgcttt cgatcggcta caatgcggag atcgtggcac
     5821 ttttttcggg catcatcgag cagaccaaga cggagcgacc ggggtatcag cctttcgtgt
     5881 caggcatgtt gcttcatttg ctgggatgcg tttattctct gagcagacag caatccttca
     5941 gtaatgcgga tggagttaaa agcaagatgg agcaggccat catgttattg cgttcgaaga
     6001 tcgatgaagc catcgatatt catgagattg cggaggagct gaatgtcagc tatgcgtggt
     6061 tcagaaagtg gtttaagctt tatacggggc tcgcgcctca gcaatacgtt atccagctta
     6121 aaatggaaaa ggcaaaggtc cttctggcgg actctggaaa ttccatcaag gggatcgctt
     6181 atcaactgcg attcgaacat ccactttatt tttcaaaatt atttaaggaa aaggtcggcg
     6241 tgtcgccgga tctctaccgg aagcagtgcg gtgggagtta ggggattgcc gtgaggcggg
     6301 actggcagga tgcgcagaac cggtcgcggc tgttgtcgat ggtggcgatc ttttcattgg
     6361 cgtcattcat gatgcaatgc ggattcgggc aatggggaag gccgaggttg tggcctagtt
     6421 cgtgaaggac tacggtgcgg aggcgataga gcgaaggggc atgggtgtga gttaggatgg
     6481 cggaagtttg ctgaagacct tgatctcgac gcgcatattc tgatccatct cggcggtggt
     6541 agtgggatgg gcgttcaccg gccgggcgtt gccgaggcct ttatagcgca gacgcgtgga
     6601 gtcgaaacca ctgttgatga gcatatcgta aatatatttt gcacgctgga ccgagagctt
     6661 gaacagcggg tcatgagggg gtaatggggg accgggcgca ttacagaagc cgtcgatctc
     6721 gagaatgcgg tcgctaaagc tgcgaagact gctgatggtc gaaggcatgg cttcacgggc
     6781 ggcatcggtg aggacggggg tattggcgat gaagttgata ttgttcaatg agataacagt
     6841 atcgggttcg tcgggagaac gtacgcgcgg gggagtgtcg atgcgtgcga cggggggcgc
     6901 gggtttgtca tgataataga cgacgacgac tgcgcggcgg ctcaggctgt cgtcgccggg
     6961 aaggggttcg gatttgccgc ggccttcgaa gcggatcgac ggcaagtttg aggaaaggat
     7021 attctgcaga gtggcttgcg tcgagcgtgc gcgacggatc gagagctgtt ggttataggc
     7081 gacgctgcct ttgatatccg tatgcccgat gatggatacg ctatcggggg agaggcgggc
     7141 tatttggtgc aaaagatcga gatcctgggg accgagatcg ctgcggtcga aggcaaaatg
     7201 cacgatcagc gtatcggatc ttttttgctg tgcatgggta gtgagcaccg ccagtggcag
     7261 gaagaacaag agtaccgatt tgagcaggac aggcatgggt gtaatttaag gaaaaacggg
     7321 gacctgtcta agggagagcg gcgaaatcca cgtcgggcgg ggactggcgg gagacgggtc
     7381 gcgtgaacca ggtgtcgagc ggcgtgggtg tctggatgaa tcctccatta cgcagccaaa
     7441 agccatcggt ggtcgaaccg ccggcatagt cgagacgata acctttccgt gcggtgttgt
     7501 cgccggtgaa acgcgcgcgg gtgatggggg tccagtggcc tgcggagtcg cgggtccatt
     7561 gattcgaaaa aagaacgcga cggccctggt cgccgtagtc gggttcgaag ttttccagga
     7621 aagaatgaag atgctgaagc cacgtatggg tcgcggggcg atcccagctg gcgatgagga
     7681 gccaatgggc ttgctcggga gcgtagaacc aggcggtgaa ccgggtgatg gaggaggcgg
     7741 ttgtgggcgt ggcggtgggt gtggacgtgg cgggtgtggc gacgggtgtg acgacgggtg
     7801 tggcggcgcg tgcggaggcg ggtgtggagg ccggtgcggc aggtgcggcg ggtcccgcgg
     7861 cggaggacag tggttgcgcg cggagcagga agcgataggt gcggccggcg acccagggat
     7921 agacgagata gctttgaccg ccggatcctt cattgccgaa agagccggtg tggacatcct
     7981 gtcctttttt tacgagacgt actttgaggc tgtcggggat ggcggatgga ttgtccgtgg
     8041 tgaagggact ccagactgaa aaaagaatgc ggcgctcggt cgcgctgttg acctgcatac
     8101 cgaaatagcc gacgctga
//
LOCUS       DASYSV010000442         7337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1753433, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000442 DASYSV010000000
VERSION     DASYSV010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753433"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VGQ51_13610"
     CDS             complement(<1..432)
                     /locus_tag="VGQ51_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEV9037664.1"
                     /translation="MLPNHRRDKILELLKEDGSAKVVDLARLFKVTEVTIRQDLEKME
                     RDSLLIREHGGAYLKNIEAQVKSFELSHQENLDKKERIAVKCLEFIESGDSIILDSGS
                     TTTEIAKKLKGFKNLTVITNALNIAMMLGTEPGINLIVTGGE"
     gene            complement(518..1159)
                     /locus_tag="VGQ51_13615"
     CDS             complement(518..1159)
                     /locus_tag="VGQ51_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9037665.1"
                     /translation="MIRSKIVRVLIIDDHPLIVAGMQKMLASIAEIEIAGAAGSAFEA
                     LKTLKTVPVDIALLDINLPDINGIELCKKIKKEFPAVKVLAISTYCDRSYVSRMIENG
                     ALGYLAKSADADEILKAIRTASEGRLYLSLSTEHLLQSPAVMTPPGSPPALTRREKEV
                     LQLIAEGLTNPQIAEKLFISPLTVDSHRKNLLTKLNVNNTAALVRLAVEQRLL"
     gene            complement(1174..3186)
                     /locus_tag="VGQ51_13620"
     CDS             complement(1174..3186)
                     /locus_tag="VGQ51_13620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV9037666.1"
                     /translation="MLQRLLISICTISCCFFARNAFAQDRIIDSLQSRLSSTALPDTD
                     RIDVMLALGDRLGYSDGALARKYLDSAYALASSKKLYLKIGDALSSYAQAYYRTNNLD
                     TALDLLRRADTVYGMADPDKAKGALVSSKMNIATVLRTMGDFSTAIGMYLKGVDVLDR
                     RDIPDWRIKLMTAYMNIGLVYNEFNEYDRALFYHRKALDLVNPEVAGDIKTYYLRLHR
                     IHDLIELKQFDSARYYLGKEDPLYKKLGQSDILSQMYSNWGFYYLGVRNEDAAITAFQ
                     NSYRYALTSNNSFRQVQMLSRLGEIYRNRKDYRESAQFLEKAYELSATLDDKPGEMNL
                     AKALSSIYANDLHDDRKAVAHFLAYVRLSDTLNESASRRKVNELEAQHRISRKEDSIA
                     MLQKQGHLQDLLLKRTTALSTTLIAGSLLLLSLAIVIIINFRRKHQILQQAKQLQERH
                     ILEMERQHQLVAMQSVLQGQEEERSRLARDLHDGVGGLLSGIKLSLAGMKGNIYLPEQ
                     GAQSLGNVISQLDHSISELRRVSHNMMPEALIRYGLREALENYCANINVSGGIRVQLQ
                     TYGMEERLEQSTEIVLYRIVQELLNNVIRHARAGQVLVQLIRKEDRFSLTVEDDGIGF
                     DHGKVGSMMGAGIANVRARAEYLGGTVDIHSAAGQGTSVNVEGNCA"
     gene            3325..5712
                     /locus_tag="VGQ51_13625"
     CDS             3325..5712
                     /locus_tag="VGQ51_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9037667.1"
                     /translation="MKRILNTGMLPVILLVLCTPVQAQLWKKIKEGAKNAVDQASSKP
                     APKPVAGNPVTPPAHVADTVPAAPSAAAGVDYANYDFVAGDRIIFQPDVSGEADAELP
                     SHFIVTSGKAEMQTFDGEKVLHLDAGTGACVLPMMNSVHYLPDQFTLEFDILFEGLQP
                     TKFEPMMDFLVTFHSPEDENYNGYPKYAFRLSANDRYRWAGQPADAVPAPLKTSLAIP
                     GVWHHVAIYLNKALGKVYIDQYRLAATNAVPQGAGHLAIHSNGKYGLFIRNVRIAEGG
                     DDKYKKIITEGKFITHGILFDVNKATIRPESTGALREIATLMKAHTDLQFEIDGHTDS
                     DGSPDGNMRLSQQRADAVKEALVRMGIDAGRLTTKGFGASKPMDTNGTAEGKANNRRV
                     EFVRHGAPAGAGAGVSHGPGGAAVKTAGASRRPCCWIVNYNSQANHETGPINVLRKGD
                     GTVWTFRPGQELQQVPGLTDVVAIAAGAMHVLALKSDGTVWGWGMNHRHQLCVDYTNG
                     HVYDPVQVQGITDAVAITTDESYTCYALLRDGSVVTWGSAAQGMAGDGGPLVSNIDAT
                     KNASRNTPVRIPGIANAIDISGAYALLADGTVMAWGDGQNGRLGNGSIKSSPVPVKVS
                     GITNAVAISCGYYGGTAELADGSLWAWGMAWSGMLGNGKDPIDGTDVSGSRKYSSVPV
                     RVKSISHPLSFSGNHVTHFALMANGDVLGWGDARLGGIATKSTEYALTPMKLAGLHDV
                     VAVQSGNMTGFALQKDGTVMGWGTDVVKDGLYKNSYTPVRVASLGSLAPQDCAVR"
     gene            complement(5850..6842)
                     /locus_tag="VGQ51_13630"
     CDS             complement(5850..6842)
                     /locus_tag="VGQ51_13630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV9037668.1"
                     /translation="MPDAHAIEDMACCMLAENLPAQFAWFAEIGPDRRHISVRSEFTR
                     DRTIRLAGVYPVDQFQSLVKIIGAEQPYIIDDAFQSFQIGNPERSFYEDHRIRACLAF
                     PVAKGNQTLTYIAIAVGIPHHWTASEVSLVHETAVRIDDAIRVVTQRSTDEEALRKSE
                     QRLSLALTSAKMGAFEWTLEGYRLTLPGFSREVFGLMPGEEFPTGDAYLNLIHPDDRD
                     SHRQVFEHAGASGEHYQSIYRIIRPVDGKTVWIEECGCGYKDDRTGTTGILGIHWDVT
                     QRVIAEEQTKGLLCLREERAHLMQKLNSQVSRLHKLMCDLLEPPRALESASETT"
     gene            complement(6953..>7337)
                     /locus_tag="VGQ51_13635"
     CDS             complement(6953..>7337)
                     /locus_tag="VGQ51_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037669.1"
                     /translation="SFSIIENVLETDPAFHINFASQNFYNGYYGKRRYNPNRKNSNSS
                     TSVTAAIANASKMQLMDYELEAPIEYNWRKKLKLNFTPTLAIPENPATVTTTTKTTGG
                     KNTTQTTTESLNGVFYFSVAITYTL"
BASE COUNT         1600 a         1851 c         2091 g         1795 t
ORIGIN      
        1 ttcgccgccg gtgacgatca ggttgatgcc gggttcggtg cccagcatca tggcgatgtt
       61 cagcgcgttg gtgatcacgg tgaggttttt aaaccctttg agtttttttg cgatctccgt
      121 cgtagtcgag cccgagtcga gaatgatgct gtcgccgctt tcgatgaatt caagacattt
      181 gacggcgatc cgttcctttt tgtccaggtt ttcctgatgg ctgagctcga agcttttcac
      241 ctgggcttcg atattcttga gataggcgcc gccatgttcg cggatcagca gactgtcccg
      301 ttccatcttc tctaagtcct ggcgaatggt cacctcggtg actttgaaca gccgggccag
      361 atcgacgacc ttcgccgatc cgtcttcctt caaaagctct aatatcttgt ctctccgatg
      421 attaggtagc atgatcgaaa gtattcgaag ctaaacgaag ttgttttctt tcgaattaga
      481 caaatataga gaaaaatcga agataaacga aaattgatta taatagtcgt tgttcgacgg
      541 ccaggcggac gagtgcagcc gtattattca cattcagttt ggtcaggagg ttctttctgt
      601 gactatcaac cgtgagcggg ctgataaata atttttccgc tatctgggga ttggtaagtc
      661 cttctgcgat gagctgaagc acctcctttt cgcgccgggt aagtgcgggc ggtgagcccg
      721 ggggcgtcat cacggcagga gattgtaaca gatgctcggt ggaaaggctg agatagagac
      781 ggccttcgct tgctgtccgt atggctttga ggatctcgtc ggcatccgca ctttttgcaa
      841 gatagcccag ggcgccattt tcgatcatcc tcgatacgta gcttcggtcg cagtaggtac
      901 tgatggcaag aactttgacg gcaggaaatt ctttttttat cttcttgcac agctcgatcc
      961 cattgatatc ggggagattg atatccagca gggcaatgtc cacggggacg gttttcaggg
     1021 tcttcaatgc ttcgaaggcg ctgccagccg cacccgctat ttctatctca gcgatggagg
     1081 ccagcatctt ctgcattccg gcgacgatca gcgggtggtc gtcgatgatc aggactctca
     1141 ctatttttga tcttatcatg tgttgtagcg aaattatgca cagttgcctt cgacattgac
     1201 cgatgtaccc tgaccggcag cggaatggat atcgacggta cctcccaggt attccgcccg
     1261 ggccctcaca ttggcgatcc ctgcacccat catggaaccc acttttccgt gatcgaagcc
     1321 gatcccgtcg tcttctacag taaggctaaa acgatcttct ttcctgatca gctgtaccag
     1381 cacctgtccg gccctggcat ggcggatcac attattcagc agctcctgga cgatgcggta
     1441 aagcacgatc tccgtcgact gttcgaggcg ctcttccatt ccatacgttt gtaattgcac
     1501 ccggataccg ccggaaacat tgatattggc acagtaattt tccaatgcct ccctgagacc
     1561 ataccggatc aatgcctcgg gcatcatatt gtgcgaaaca cgacgaagct cgctgatgga
     1621 gtgatccagt tgcgagatga catttccgag ggactgcgcg ccttgttccg gaaggtatat
     1681 atttcctttc atgccggcca acgacagttt gatacccgac aaaaggccgc ctactccatc
     1741 gtggaggtcg cgggcgagcc ggcttcgttc ttcttcctgg ccctgcaata ccgactgcat
     1801 ggctaccagc tgatgttgtc tttccatctc cagtatgtgt cgttcctgga gttgcttcgc
     1861 ctgttgaagg atctggtgtt tccttctgaa gttgatgatg atgacgatcg ccagcgacag
     1921 gagcaagagg ctgccggcta tcaatgtcgt actcagcgcc gtcgtccgtt tcagaagcag
     1981 gtcctgcaga tgcccctgtt tttgaagcat cgcgatgcta tcctccttcc tgctgatgcg
     2041 atgttgcgcc tccagctcgt tgaccttacg ccggcttgca gactcgttga gggtatcact
     2101 gagacggaca tacgctaaga aatgagcgac ggccttccgg tcatcatgaa gatcattggc
     2161 atatatcgaa gacagggcct ttgcaagatt catttcgccg ggtttgtcgt cgagggtggc
     2221 gctaagctca tatgcctttt ccaggaactg cgcgctttcg cggtagtcct tcctgttccg
     2281 gtagatctct cccagacggg acagcatctg cacctgtctg aaactgttgt tcgaagtcag
     2341 ggcataccgg taagagttct ggaaagcggt gatcgcggca tcttcgttcc tgactcccag
     2401 atagtagaaa ccccagttgg agtacatctg tgaaaggata tccgattggc cgagcttttt
     2461 atagagcgga tcttctttcc cgaggtagta cctggccgag tcgaattgtt tcaattctat
     2521 caggtcatgg atacgatgca accgtaggta ataggtcttt atgtcgcccg ccacttccgg
     2581 atttaccagg tccaacgctt tccggtgata gaacaacgcc ctgtcatatt cgttgaattc
     2641 gttatagacc aaaccgatgt tcatgtacgc cgtcattagc tttatccgcc agtcgggaat
     2701 atcgcgtcga tcgagaacat cgaccccttt gaggtacatc ccgatggccg tggagaaatc
     2761 ccccatggtc ctgagcaccg tggcgatatt cattttgctg ctgaccaggg ctcctttcgc
     2821 tttatccgga tcggccatgc catagacggt atccgcccgg cgcagcaggt ccagcgcagt
     2881 gtcgaggtta ttagtgcggt aataggcctg agcgtaggaa gacagggcat cgccgatctt
     2941 caggtacaac tttttactgc tggcgagcgc ataagcgctg tccaggtatt tcctggcaag
     3001 agcgccatcg gaatatccca gccggtcgcc gagggccagc atgacatcga tgcggtctgt
     3061 atccgggagt gctgtcgagc taagtctcga ttggagacta tctatgatcc ggtcctgcgc
     3121 gaacgcgttt cgtgcgaaaa aacagcacga aatagtacag atcgatatta gcagccgttg
     3181 gagcatacaa ctaaactcat gaaaataaga ctttcttcca aggaatttaa aaaaatcccc
     3241 cgaagggggg ataggttaaa aacctgtttt cccgggattg tacgggatcg cgcgagaacc
     3301 gagttttgta ccaatttctc atctatgaaa agaattctga ataccggtat gttgccggtg
     3361 atcctgcttg tcctctgtac gccagtgcag gcccagttgt ggaaaaagat caaagagggg
     3421 gcaaagaacg ctgtcgacca ggcgtcatcg aagccggcgc caaagcctgt cgccggaaat
     3481 ccggtcactc ctccggccca tgttgcggac accgtccccg cggctccgtc cgcggcagcc
     3541 ggggtggatt atgccaatta tgactttgta gccggggatc ggatcatctt tcagccggat
     3601 gtcagcggcg aggcggatgc ggaattgcca tcgcatttta tcgtcacctc cggcaaggcc
     3661 gagatgcaga cgttcgacgg ggaaaaggta ttacatctcg acgcaggtac gggcgcctgt
     3721 gtattgccta tgatgaattc ggtgcactat ctgcccgacc agttcaccct ggaattcgat
     3781 attctattcg agggattgca gcccaccaaa tttgaaccga tgatggactt cctcgtaacg
     3841 tttcactcgc cggaggatga gaactacaac ggctatccga aatatgcctt ccggctttcc
     3901 gccaacgacc gctatcggtg ggcgggccaa cctgcggatg ccgtgcctgc accgttgaag
     3961 acttctcttg ccatacccgg agtctggcat catgtcgcga tctatctgaa caaggcgctt
     4021 ggaaaagtat atatcgacca ataccgtctc gccgccacga acgcggtgcc acagggcgca
     4081 ggtcatctgg ccatccacag taacgggaaa tacggtctgt tcatcaggaa tgttcgtata
     4141 gctgagggag gcgatgacaa atataaaaag atcatcaccg aagggaagtt cattacccat
     4201 ggcatcctgt tcgacgtgaa taaggcgacc atcaggccag agtctacggg cgcgctgaga
     4261 gagatcgcga cattgatgaa ggctcatacc gatctgcaat tcgagatcga cggacatacg
     4321 gacagcgatg gcagtcccga tggaaacatg cggttgtcgc aacagcgggc cgatgccgta
     4381 aaggaggcgc ttgtcagaat gggtatcgat gccggccggc tcactactaa gggattcggg
     4441 gcgagcaagc cgatggatac gaatggaacg gccgagggta aagctaataa caggagggtt
     4501 gaatttgtga gacatggcgc cccggcaggc gcgggagctg gcgtctcaca tggcccgggt
     4561 ggggcagcag tgaagaccgc cggcgcaagc cggcgtccct gttgttggat agtcaactat
     4621 aacagtcagg ctaatcatga aacggggccc atcaatgttt tgcgcaaggg cgatggtacg
     4681 gtgtggacct tccggccggg acaggagttg cagcaggtgc ccgggcttac ggatgtggta
     4741 gcgatcgccg ccggagcgat gcatgtcctg gctctcaaat cggatggtac cgtctggggc
     4801 tgggggatga accaccggca tcagctctgt gtcgactata cgaatgggca tgtctatgat
     4861 cctgtccagg tgcagggtat cacggatgcg gtggctatca ccaccgatga aagctatacg
     4921 tgttatgctt tgctcaggga cggtagtgtc gtcacgtggg gcagtgcggc gcagggaatg
     4981 gcgggggacg gagggccgtt ggtcagtaat atcgacgcca caaaaaatgc cagccggaac
     5041 acgcctgtca ggatccccgg catcgccaat gccatcgata tctcgggcgc ctatgcattg
     5101 ctggcggatg gcacggtgat ggcctggggt gacggtcaga atggccgtct tgggaatggc
     5161 tcgatcaaat cgtcgcctgt ccctgtgaaa gtctccggta tcacgaacgc ggtagccatt
     5221 tcatgcggct attatggtgg cacggcagag ctcgccgacg gctcgctgtg ggcgtgggga
     5281 atggcctggt ccggcatgct ggggaatggc aaggacccga tcgatggaac ggatgtctcg
     5341 ggctcgagaa aatatagttc cgttccggtg agggtaaaga gcatcagcca tccgctttcg
     5401 ttcagcggta atcatgtgac tcattttgcc ctgatggcaa atggcgatgt gttgggttgg
     5461 ggggacgcgc gtctcggcgg cattgccacc aaatcgactg agtacgcgct gacgccgatg
     5521 aagcttgccg gacttcatga cgtagtcgcg gtgcagagcg ggaacatgac gggatttgca
     5581 ttgcaaaaag acgggacggt gatgggctgg gggaccgacg tcgtaaagga tggactttat
     5641 aagaattcct atactcccgt tagggttgct tcgctgggca gtctcgcccc acaggattgc
     5701 gcggtaagat aattattcgg tacgcaaaga tctcacgggg ttggccagcg cggcgcggat
     5761 cgatgaggag ggcgacggcc atgccgacgg gaaggcccgc aatattgatg tcccctactg
     5821 ttacggaaat tgtgcggtta tgatacagat cacgtggtct ctgatgctga ttccaaagcc
     5881 ctgggaggtt cgagcagatc gcacatcaat ttatgcagac gggatacctg tgagttgagc
     5941 ttttgcatga gatgggcgcg ttcctcgcgc aaacaaagca gaccctttgt ctgctcttcg
     6001 gcgatcaccc gctgtgtgac gtcccagtgt atgccgagga tgccggtagt tcccgtgcgg
     6061 tcatctttat agccgcatcc gcattcttct atccacaccg ttttcccgtc gacggggcgt
     6121 atgatcctat aaatgctttg ataatgttct ccgctggcgc ccgcatgttc gaacacctgc
     6181 cgatggctgt cgcggtcatc cggatggatc aaattcaaat aagcatcgcc ggtagggaat
     6241 tcttctccgg gcataaggcc gaagacctcc cgggagaacc cgggtagcgt cagacggtat
     6301 ccctccaatg tccattcaaa ggctcccatt ttggcggagg tgagtgcgag gctaagccgt
     6361 tgttcgcttt tgcgcaaggc ttcttcgtcg gtgctgcgtt gcgtaacgac ccggatcgcg
     6421 tcgtcgatgc gtacggcagt ctcatggacc agcgagactt cactcgccgt ccagtggtga
     6481 gggatgccga ctgctatggc gatgtaggtc agcgtctggt ttcctttcgc gacgggaaat
     6541 gccaaacatg cgcggatgcg atgatcttca taaaaggaac gttcgggatt accgatttgg
     6601 aaggattgaa aagcgtcgtc tatgatatag ggctgttccg ccccgatgat cttcaccagg
     6661 gattgaaact gatcgacggg atacacgccg gcgagtcgta tcgtacgatc gcgtgtgaat
     6721 tcactgcgga cggaaatatg ccggcggtcg gggccgatct cggcaaacca ggcgaattgg
     6781 gcgggtaaat tttcggcgag catgcagcag gccatgtcct ctatggcatg agcatccggc
     6841 atcgcatgca acgtatccgc caatttgagt atgaatgcca gtcgcgcggt atcgtgagta
     6901 tcatattgcg tacatcaaaa taagcaattt aggcatcatg gcaaaaaggt tcttacaacg
     6961 tataagtgat ggcgaccgaa aaataaaaga cgccgttcag gctttccgtc gttgtttgcg
     7021 tcgtgttttt tccacccgtg gtttttgtcg ttgtggtaac ggtggccgga ttctccggta
     7081 ttgcaagcgt cggagtaaag ttgagcttga gttttttccg ccagttgtat tcgatgggcg
     7141 cttccagttc ataatccatc aattgcatct tcgaggcatt ggctatcgcg gccgtgacgg
     7201 acgtcgagga attgctgttt tttcggttgg gattgtaccg tcgtttgccg taatagccgt
     7261 tgtaaaagtt ttgcgaagcg aaattgatat gaaaggctgg atcggtctcc agcacgtttt
     7321 ctatgatgga aaaggaa
//
LOCUS       DASYSV010000443         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2267935, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000443 DASYSV010000000
VERSION     DASYSV010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267935"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..486
                     /locus_tag="VGQ51_13640"
     CDS             58..486
                     /locus_tag="VGQ51_13640"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEV9037670.1"
                     /translation="MKRLFLFASAVILSASVFAQSKKTEDVIKFKELTYDFGKIKQGE
                     PVKHDFAFTNVSNSPVIIESAVASCGCTTSFKPEGAIPQGKSDKITASFDARTAGTFT
                     KTITIKAAGTDLPLQIRFTGEVLTADAYAKYEATKGKSGR"
     gene            576..1772
                     /locus_tag="VGQ51_13645"
     CDS             576..1772
                     /locus_tag="VGQ51_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV9037671.1"
                     /translation="MLTISQRGQQMPASPIRKLVPFAEAAKKQGIKVFHLNIGQPDIE
                     TPKMALDAVRHFDFKVLEYSHSAGNETYRRKLAAYYHRVGIDVDYQQIIITTGGSEAI
                     LFGFMACLDAGDEVIIPEPFYANYNGFATAAGVMVKPLTSYIGNGFALPPASAFEELI
                     TPRTKAIVICNPNNPTGYLYSREEMETLKDIVIRHGLYLMSDEAYRDFCYSGVHISAM
                     NLKGAEDNVLMMDTISKRYSACGGRIGALVTRNQAVLDSVMKFAQARLSPPSFAQILG
                     EAATDLPADYFDATRAEYLQRRDVLVNRLNRMPGVCCPNPGGAFYAMASLPVDDTDVF
                     CRWLLESFSYQNQTVMLAPAGGFYGTPGLGKKEVRLAYVLNTTDLGKAMDCLEKALQE
                     YPGRTN"
     gene            1960..2955
                     /locus_tag="VGQ51_13650"
     CDS             1960..2955
                     /locus_tag="VGQ51_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV9037672.1"
                     /translation="MIDRRKFLKHTGQLGFAGLLFPHLSSANALFKHSARLSAGAFPD
                     VAWLSHPIGLQLYTLGNLMITDPKGTLQKLAAIGYKEVESAGSQKGNFYGFTPKDFSA
                     MVKEAGMHWRFAHVPGAPFTIAQMMHMAKTHDDSVRIQKYADQMKNRPKTSNLKEDHQ
                     LLADQAAEGGLSYLVCSSIPVHTFDEIKTAVDVFSKAGEACKKNGVQFAYHNHTTEFD
                     EVEGHRPFDYILNNTDKDLVKMELDLAWATKAKQDPVELFKLHPGRYPLWHVKDLDKI
                     KQQPAVVGTGIVDFKRIFDNAKESGMKYFFVEQDGAPQPLENVTQSFNYLKNMQV"
     gene            complement(2964..>3630)
                     /locus_tag="VGQ51_13655"
     CDS             complement(2964..>3630)
                     /locus_tag="VGQ51_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037673.1"
                     /translation="PLFVENNFSNFYSNLFQNQWFGAGKSVAMLEYAWNVSPSNAVKC
                     DPCVAAAPSTQDLVQAGVWWVGSRRNGYDPENDNADDVGKVYFTRLHVRYNRKAFPQD
                     LFFQETANNTSFQARYIITHPATGDLSCDAGRKYLKELKERRKDELKMLTYLTGKGFD
                     DWDVVASREEEKSLPEADSYAAIAADIRKYPPVGHGMMIAGIGVLGVLSLIGIAIRGR
                     WKT"
BASE COUNT          889 a         1025 c          875 g          841 t
ORIGIN      
        1 cctcggcgcc ggaaaatgcc tctgtggaat tactaaaaca aataaattga tcaaaaaatg
       61 aagagactat tcctgtttgc cagcgctgtc atcctttccg ccagcgtatt tgcccagtcg
      121 aaaaagaccg aagacgtaat aaagttcaaa gagttgacct atgatttcgg caagatcaag
      181 caaggcgaac ccgtcaagca cgattttgcc tttaccaatg tcagcaattc cccggtgatc
      241 atcgagtcgg ccgtcgcctc ctgcggctgt accacctcct ttaagccgga aggcgccatc
      301 ccgcaaggaa aaagcgataa gatcaccgcg tccttcgacg cccgcacggc tggtacgttc
      361 accaagacca tcaccatcaa ggctgccggc accgatctgc cgcttcagat cagatttacc
      421 ggcgaggtgc tgacggcgga tgcttatgcc aaatacgagg ctacgaaggg gaaatccggc
      481 cgctagggct cttctgatct caatgaattg aaaaaccgtc tgcaacggtt tttttttgtg
      541 ccatcgggct tttccgtttt gtttattttt gccgaatgtt aaccatcagt caacgcggac
      601 agcaaatgcc ggcttcaccg atcagaaaac tcgtaccctt cgccgaggcc gccaaaaagc
      661 aaggcattaa ggtttttcat ctcaatatcg gtcaaccgga catagaaact cctaaaatgg
      721 cgttggacgc cgtccgacat ttcgatttca aggtgctcga atacagccat agcgcgggca
      781 acgaaactta ccgccgcaag ctggcggctt actatcatcg ggtaggtatc gatgtggatt
      841 accagcagat catcatcacg accggtggct cggaagccat cctattcggg ttcatggcct
      901 gtctcgatgc aggagatgaa gtcatcattc ccgaaccctt ttatgccaat tataacgggt
      961 tcgctaccgc ggccggtgtg atggtcaaac cgctgacttc ctatatcggc aatggtttcg
     1021 ccttgcctcc ggcgtcggct ttcgaagaat tgatcacacc ccgcacaaag gccatcgtca
     1081 tctgcaaccc caataacccc acgggctatc tgtacagccg cgaggaaatg gagaccctga
     1141 aggatattgt catccgtcat ggcctttatc tgatgtccga tgaggcctac cgcgatttct
     1201 gctacagcgg cgtacacatc agcgcaatga acctcaaggg cgcagaagac aatgtgctca
     1261 tgatggatac catctccaaa cgatacagcg cctgcggggg gcgcatcggt gcgctcgtta
     1321 cccgcaacca ggccgtcctg gattcggtga tgaagtttgc gcaggcgagg ttgagtcctc
     1381 cgtcgtttgc gcaaatactc ggggaagcgg ctaccgacct tcccgcggat tatttcgacg
     1441 ctacccgggc agaatacctg caacgtagag acgtgctcgt caatagactc aatcgtatgc
     1501 ccggagtctg ctgtcccaat ccgggcggcg ccttttatgc gatggccagc ctgcccgtcg
     1561 atgatacgga tgttttttgc cgatggctac tggaatcctt ctcctaccaa aatcaaacgg
     1621 tcatgctagc gcccgccggt ggattttatg ggacccccgg tctgggaaaa aaggaagtga
     1681 ggctcgccta tgttctcaat accaccgatc tcggtaaggc catggactgt ttggaaaagg
     1741 ctctccagga ataccccggc aggacgaatt aaatccttat tttaacgttt tgatattatt
     1801 aagggcattt gcgatcgatc cgggggactt aaatttgtgc gcaccacatc ggatctcttt
     1861 aagagttttc atatggcaag caatcgttag acggccttct gtttccacag ggggtctttt
     1921 ttatgcccaa aatccgttac atttagtaaa agtcttgcca tgatcgaccg ccgaaaattt
     1981 ctgaaacaca ccggccagct gggcttcgcc ggtcttcttt tccctcatct ctccagtgct
     2041 aacgcgctat ttaagcacag cgcccgcctt tccgccggag ccttccctga cgtcgcctgg
     2101 ttatcgcacc ccatcggctt gcagctctac acgctgggta atttaatgat caccgatccg
     2161 aagggcaccc ttcaaaagct cgccgccatc ggctataaag aagtggaaag cgcaggttca
     2221 caaaagggta atttttacgg attcaccccg aaagacttct ccgccatggt taaggaagca
     2281 ggcatgcact ggcgcttcgc gcatgtaccg ggtgcgcctt tcactatagc ccagatgatg
     2341 catatggcca agacccacga cgactccgtg cggatccaaa aatatgctga ccagatgaag
     2401 aacaggccca aaacgtccaa cctcaaggag gatcatcagc tactggccga ccaggcggcg
     2461 gaaggcgggc ttagctatct ggtctgctcc agcatccccg tacacacctt cgatgaaata
     2521 aagacagccg tcgacgtctt cagcaaggcc ggcgaagcct gtaaaaagaa tggcgtacag
     2581 ttcgcctatc acaaccatac caccgagttc gatgaggtgg aaggacatcg tcccttcgat
     2641 tatatcctca ataacaccga taaagatcta gtgaagatgg agctcgatct ggcctgggcc
     2701 accaaggcta aacaggatcc cgtcgagctc tttaagctgc atcccggccg ctatcccctc
     2761 tggcacgtaa aagaccttga caaaatcaag caacagcccg cggtcgtcgg taccggtatc
     2821 gtcgatttca aacgcatctt cgacaacgca aaagaatcgg ggatgaaata tttcttcgtc
     2881 gaacaggacg gtgcgccgca gccgctcgaa aacgtaactc aaagcttcaa ttacctgaaa
     2941 aatatgcagg tctgaccgct tatctatgtc ttccaccggc cccggatggc gatgcctatt
     3001 aacgatagca cgcccagtac tccgataccg gcgatcatca tgccgtgacc tacaggcgga
     3061 tatttccgga tgtcggcggc gatggccgca taggaatccg cttccggaag actcttctct
     3121 tcttcccggc tggcgacgac atcccaatcg tcgaaacctt tccccgtaag ataggtcagc
     3181 atcttcaact cgtcctttct cctttccttc aattctttca ggtattttct acccgcgtcg
     3241 cagctgaggt cgcccgtagc gggatgcgta atgatgtacc gtgcctggaa actggtgtta
     3301 ttcgccgtct cctggaagaa caggtcctgg gggaacgcct ttcggttata ccgcacatgc
     3361 agccgtgtga aatagacttt gccaacatca tccgcattat cgttttcggg atcatatcca
     3421 ttcctgcgcg atcccaccca ccatacaccc gcctgcacga ggtcttgtgt ggaaggcgcc
     3481 gctgcaacac agggatcgca cttcacagcg ttggaaggac tcacattcca ggcatactcg
     3541 agcatggcga ctgactttcc tgctccgaac cattggtttt gaaaaagatt ggaataaaaa
     3601 ttcgaaaaat tattttccac gaacaaagga
//
LOCUS       DASYSV010000444         3457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1115813, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000444 DASYSV010000000
VERSION     DASYSV010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115813"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /gene="gldC"
                     /locus_tag="VGQ51_13660"
     CDS             complement(<1..275)
                     /gene="gldC"
                     /locus_tag="VGQ51_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HEV9037674.1"
                     /translation="MTTSSISIDVSLDKDRIPEDIRWNASDSSTENARQAKAMMLAFW
                     DGAEKTALRIDLWTKEMMVDEMADFFYQTFMTMAETYQRATQDPTLTG"
     gene            complement(328..1440)
                     /locus_tag="VGQ51_13665"
     CDS             complement(328..1440)
                     /locus_tag="VGQ51_13665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9037675.1"
                     /translation="MFMLLDGRPLQYAAPDNERTLLILRIVGSLAREHGVKCLLLVDQ
                     TWRPGSLPGIAEEEVLIKRAFPGGIGWRLWYDWQIPRTVRKQRPSMVMLTGGIAAGPL
                     PVPQTIWFPVCANPKEIKKPVTLYAPRLAETIRRGDCFFCFSEKDREWLVSGGRVAED
                     RLGTIRPWPTVSARPLSHDDRTAIKNRVAHGKEYFFADMSETGEEEMVHLLKAFSIFK
                     KRQLSNLQLVVAGEPRGDIKEKLETYKYRQDVHWCGPSDVRDGRVPAAAYSALYLSSD
                     DSPGTPVIDSWATGVPVVVTAGTRLAEMAGDAALATSSEDPTALAGHLMSVYKDEILR
                     GRLIEKGFSRLPEFAAGQTIARVWAVIDRAFANIQH"
     gene            1656..2852
                     /locus_tag="VGQ51_13670"
     CDS             1656..2852
                     /locus_tag="VGQ51_13670"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HEV9037676.1"
                     /translation="MNRVYTCLFIIIILIVSIPWVAGAQCNCSASVPATPITYYASFP
                     ATKQPSTTITFPQFNPAIGMLSCLTLTDTVSGVTTTSAYNTAPVPVTYKFLLNISDDL
                     EGPAGGGISESNLYSKIYGPTTLQRLGLPGDSVTYGPDTLYNSSIGSGSTSSNTAPYL
                     GNGTVDFIYSLSGGLVTTQGGTNYGQQITTQYWGNFSLTYYWCPATSLSTTIRDFTAT
                     PDGNIILLQWLTENQQPNTQYEIQVSQDGKNFYAIGQAEGDASATGTAAKYQYRYNPD
                     PSYPGKLYFRVMETDPSGKISLSAILILDPKAPVSSGPVSYQTFPNPATNSLQVLFNG
                     AQTGRFLVELVATSGQVVRKEAVTLAGSNQITLDLAPQPARGLYFLRTTDLSHNRNYV
                     SKVFIQ"
     gene            2940..>3457
                     /locus_tag="VGQ51_13675"
     CDS             2940..>3457
                     /locus_tag="VGQ51_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HEV9037677.1"
                     /translation="MEYNTTRNHLVMREYGRHIQKMIEYLLSLEDKEARQRNAHAVIE
                     LMGFLNPHLKNVEDFRHKLWDHLFLISDFKLEVASPYPIPTRETLRARPKPLKYPKRY
                     PKFSHLGKNLELVINKALAEENPDKRSGFANAVAYYMKLAYNNWHKETVHDDAIQSEL
                     NQLTAGQLEFTNT"
BASE COUNT          802 a          974 c          877 g          804 t
ORIGIN      
        1 ccggtgagcg tcggatcctg ggtcgcccgt tgataggtct cggccatggt catgaaggtc
       61 tgatagaaaa aatcagccat ttcatcgacc atcatttctt tggtccagag atcgatccga
      121 agggccgttt tttcggcgcc gtcccagaag gccagcatca tcgctttggc ctggcgggca
      181 ttttcggttg aggaatccga ggcattccag cggatgtctt cgggtatccg gtccttatcc
      241 agggacacat cgatactgat agaagaagta gtcatatgca aaatatgtgc acccggagag
      301 tccggtagca agccgcaatt tactggatta atgctggata ttcgcaaacg cccggtcaat
      361 aaccgcccaa acccgggcaa tcgtctgtcc ggcggcaaat tccggcagcc gggagaaacc
      421 cttttcgatc aatcgtcccc gcaggatctc gtccttgtat accgacatta agtgacctgc
      481 cagcgcggtc gggtcttcgg aggaggtggc aagggcagca tctccggcca tttccgccag
      541 ccgggtgccg gcggttacca ccacagggac tccggtcgcc cacgaatcga tcaccggtgt
      601 tcccggtgaa tcatcggacg ataggtaaag cgcggaatat gcagcggccg gcacccttcc
      661 gtcgcggaca tcggacggac cacaccaatg gacgtcctgc cggtatttat aggtttccaa
      721 tttctccttt atatcaccgc gcggttcacc tgccactacc aactgaagat tggacagctg
      781 acgtttcttg aagatcgaga atgctttcag caggtggacc atttcctcct ctcccgtctc
      841 cgacatatcg gcgaaaaaat attccttccc atgtgcgacc ctgtttttta tggccgtccg
      901 gtcgtcatga gacaatggcc tcgccgatac ggtgggccag ggccttatcg tcccgagcct
      961 gtcttctgct acacggcccc cggagacaag ccattcccgg tctttttcag aaaaacaaaa
     1021 aaagcaatct ccccgtctga tggtctccgc gagccggggg gcatagagcg taacgggctt
     1081 ttttatttct ttcggattgg cgcacacggg gaaccatatg gtttgcggca ccggcagggg
     1141 tccggcggct atccctcctg ttagcatgac catggatggg cgttgttttc taacggtgcg
     1201 cgggatctgc cagtcatacc agagccgcca tccaatgcca ccgggaaaag cccgtttgat
     1261 cagcacctct tcttcggcta tacccggaag cgacccgggc cgccaggtct gatccacaag
     1321 caggagacat tttacgccgt gttcgcgggc aagtgagccg acgatccgaa ggatcagaag
     1381 ggttctttca ttatcgggcg cggcatattg aaggggccgg ccatccagca gcatgaacat
     1441 actgcaaagg taggaagggg gtcctagtgc ttatctccat ttttttatcc acaaccagga
     1501 aaaaccaagc aactgataat caatttcttt aatatacaaa acgtttaaca ggaatgtggt
     1561 ttttacggtc gggtcgtctt aaatagcgcc gcttggtctc attcttgctc ttatacccgt
     1621 agtattaatc cgtcacctat ctaaaactcg tgcctatgaa ccgtgtgtac acctgtttat
     1681 ttattatcat tattttgata gtaagtatac cctgggtggc cggcgctcaa tgtaactgca
     1741 gcgcatcagt gcctgcgact ccgatcacct attacgcttc cttccctgct accaagcagc
     1801 cttccaccac gatcacattt ccgcaattca atcccgctat cgggatgctc tcatgtctga
     1861 cgctgacaga taccgtttca ggcgttacga cgacaagtgc atataatacg gcgcccgttc
     1921 cggtgacgta taaattcctg ctgaatattt cggatgacct ggaagggccc gcaggcggtg
     1981 ggatatccga atccaatctg tacagcaaga tctatggccc cacgaccctg cagcgtctcg
     2041 ggctgccggg ggattccgtg acctatggtc cggataccct ttacaatagc tcgatcgggt
     2101 cgggctcgac cagcagcaac acggcgcctt atctcgggaa tggcaccgtg gatttcatct
     2161 atagcctgag cggtggtctc gttactaccc agggtggtac gaattacggt cagcagatca
     2221 ccacccagta ctggggtaat ttcagcctca catattactg gtgtcccgcc accagcctgt
     2281 cgacaacgat ccgtgatttc acggctacgc ccgacgggaa tatcatcttg ctgcaatggt
     2341 taaccgaaaa tcaacaaccc aatacccaat atgagatcca ggtcagccag gacggcaaaa
     2401 acttctatgc tatcggacag gcagaaggtg acgcttctgc aacaggtacc gctgcaaaat
     2461 accagtaccg atacaaccct gatccgtctt atccgggaaa actctatttc cgggtgatgg
     2521 aaactgatcc ttcggggaag ataagtctat cggcgatttt gatcctggat ccaaaagcgc
     2581 ccgtcagtag cggtcctgtc agttatcaga cttttccgaa ccctgcgacc aatagcctgc
     2641 aggtgctgtt caacggcgcc cagacgggcc ggttcctggt ggagctggtg gccacatcgg
     2701 gtcaggtcgt acgcaaggag gccgtcacgc tggccggttc gaaccagatc acgctcgatc
     2761 tggctcctca acctgcacgg ggtttgtatt tcctgcggac caccgatcta agccataatc
     2821 gcaactatgt cagtaaggta ttcatccaat agaaggtagc ggggcgccgc cggcggcgcc
     2881 ccgctttttg tttgcaggct gggctttttc ctgtagattt gttgatctaa tcagaacgta
     2941 tggagtataa tacgacccgg aatcacctgg taatgcgtga atatggaagg cacattcaga
     3001 agatgatcga atatcttctg agtctggaag acaaggaggc gcgtcaacgc aacgcgcatg
     3061 ccgtcatcga gctgatgggt tttctcaacc cgcatctgaa gaatgtcgaa gattttcgtc
     3121 ataaactatg ggaccatctt ttcctgatct ccgacttcaa gctggaggtc gcgagtccct
     3181 atcccattcc cacccgtgaa accctccgcg cccgtcccaa accgctgaag tatccgaaac
     3241 gctatccgaa attctcgcat ctcggtaaga atctcgagct cgtgatcaac aaggcgctgg
     3301 cggaagaaaa tcccgacaaa cgcagcggtt tcgccaatgc cgtcgcctat tacatgaagc
     3361 tggcctataa caactggcat aaggagaccg tacacgacga cgccatacag agcgagctca
     3421 accagctcac cgccggccag ctcgaattca cgaatac
//
LOCUS       DASYSV010000445         8643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_346313, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000445 DASYSV010000000
VERSION     DASYSV010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8643
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346313"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VGQ51_13680"
     CDS             <1..1011
                     /locus_tag="VGQ51_13680"
                     /inference="COORDINATES: protein motif:HMM:NF016364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEV9037678.1"
                     /translation="FVWIANNIQYDVVNMFALNFYEDPAEKIARPLKTRKGICENYAS
                     LFTAICNRVGIRSLVVEGYTKERGFVGYIPHAWSAAFLDGKWWLFDPTWGSGYVDNGK
                     FVRKMNESYFKAAPENFIATHMPFDYLWEFLYYPVTTGDFFNAKTGQDKTKPRFDFPD
                     SIRAYEAMPKEEQEEAAADRIEKNGVRNSSAADMVRHLRVDVDNYHRQQENDRQNRII
                     DSFNMATRTYNQSVNQFNLFINYYNVQFKPPKPDAEIQQMLDTAAHQMQEAKTLATTI
                     VLTPADSRVAKPLDQLKEGIATQEAHMKEMEEWLVKYLSKSKLGRRSMFYKITWFGIP
                     VN"
     gene            1022..1507
                     /locus_tag="VGQ51_13685"
     CDS             1022..1507
                     /locus_tag="VGQ51_13685"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV9037679.1"
                     /translation="MEKKEIRLAYEEYASDRELTASDGGLLETARKATGDAYAPYSRF
                     RVGAAARLANGEVITGSNQENASFPAGICAERTLLSAAASRFPGVPIETIAISYVNGQ
                     GPSDRPISPCGICRQSLQEMEQRTGRPIRLVLGGMEGRVMILPQAGQLLPLAFTADEL
                     G"
     gene            complement(1533..2549)
                     /locus_tag="VGQ51_13690"
     CDS             complement(1533..2549)
                     /locus_tag="VGQ51_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV9037680.1"
                     /translation="MVIWTLRVLDIRAEAVDTKTIFLDREDGGPFSYTAGQFLTLLFT
                     PKGHDIRRSYSICTTPGIDPTPAITVKRVPNGEISRFLLDHLRVGDTLTSLPPSGRFF
                     LGETAAVHFFLGAGSGIVPIFSLLKQALAEDARVVLISQFHDRESAPFYDSLQTLAAR
                     DGFQWIDLLTIRDGRLNNWSLGEWLFGLLPREALPGTQFYICGPPAFIRMAQFTLRTL
                     GIPERHIRKEHFTVEHIPAPPPVFDKTPRIVVIHDGANVHHRFEMKWPDTILQAAEKE
                     GLHLPFSCRAGRCSTCVARCISGSVKMSNNEVLTDKDVREGLVLTCTGYPETDVELSF
                     RQSG"
     gene            2670..3773
                     /locus_tag="VGQ51_13695"
     CDS             2670..3773
                     /locus_tag="VGQ51_13695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9037681.1"
                     /translation="MACSFKLTSLAGLSCALLLTSMGLGSLSSCKHTDAESSTLGKKQ
                     AEATIVDVLVAAPHTLTNQVEANGTVVANEYVELHPEISGRIVYLNVPEGARVSKGTV
                     IARINDADLQATLEKSKAQLDMYQKTVEREHKLLEINGINQADYDLAVSNVNATKADI
                     DYTQAQLDKTVIRAPFDGVVGLRQVSPGAYVTSANIIATVQQMEKIKVDFTVPEEYSA
                     VIHKGGVVDIELDAINNTHRKGLIIATEPQINLSSRNLKVRALLADGSAHPGAFVKVF
                     INTGVDKKAILVPTNCIIPEDRTNQVMVVKDGLAKLVAVGTGERQSGNVAITSGLNAG
                     DTVIVTGVLFARPGTPVTIRAVKTLDNASPASN"
     gene            3779..6877
                     /locus_tag="VGQ51_13700"
     CDS             3779..6877
                     /locus_tag="VGQ51_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9037682.1"
                     /translation="MNISELSLKRPVLATVMNLMLILFGVVGYNFLAVRDYPAIDPPI
                     ITVQTNYTGANPDLIENQITEPLEKNINGIPGIRTISSTSNLGSSYITVEFNLGVDLE
                     AAASDVRDKVGSAARSLPLDIDAPPIVTKADANSDFILILAVQSRTKSLMELSDYAEN
                     VLQQQLQTIDQVSAVYVFGEKTYSMRIWLDQDKMNAYGVAYSDIATALNNENVELPPG
                     KIYGNNTELTIKTLGRLTTEKDFRDVILREDSSGIVRLSDVARVEIGPQNPEQSWKYN
                     GVNAVGLAIIPQPGANNIAIADEFNRRLDEIKKANKSDLEVNVLVDNTRTIRQSLSEV
                     KETLLISLSLVVLVIFFFFRNWMIALRPLIDIPISLIATFFIMYLAGFTINILTLLGI
                     VLATGLVVDDGIVVTENIFRKLEQGMPIRKAALEGSKEIFFAVISTSLTLAVVFLPVI
                     FLQGFVGRLFREFGVVLAAAVLISSFVSLTITPVLNVYLTRKNSGHGRFYERTEPFFT
                     GMENGYRRLLTAFMKVRRMAWVIIAVCCAIIVFVMRHIQSEIAPMEDRNSIRFTVTLP
                     EGSSYSYTQAVTDQISNYLLDSVPEREFVFARTPTNNSSNASQPRIGLVEPWMRKRSQ
                     NEIANDLQRKLSRFNDARIFAIQEQTIAVGSGSKTSLPVQFVLENQDLDKMKAILPAF
                     LDSCRKDKTFSNVDVNLKFNKPELQVTVDRMKVRDLGLSTSEVISAMQAAFSGGRLAY
                     FIMNGYQYYVIAQVERQDRNEPADISKIYVRNKNGDPIPLSAVLHVESNSSPSTLYHF
                     NRYKAATISASLAEGKTVGDGIKTMQRIADKLLDASFQTALSGASRDYAESSSNIIFA
                     FVLALVLIYLVLSAQFESFRDPLVIMLTVPLALAGALLSLWVFHQTLNIFSEIGMIML
                     IGLVTKNGILIVEFTNQKREKGMPKREAVIEAASHRLRPILMTSLATSLGALPIALSL
                     GAAASSRIPLGIVVVGGILFSLILTLFVIPAVYTFISGRHKNHPTSPVEELQESEPIE
                     QI"
     gene            6874..8187
                     /locus_tag="VGQ51_13705"
     CDS             6874..8187
                     /locus_tag="VGQ51_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9037683.1"
                     /translation="MRKLYSVWTALTLSTMILQAQPLTRDEAVRIALKNNLGIELARN
                     AVAIASINNSYGIAGGLPVVTGTISDQENSPSFRQVYSNAASNKASNNAFSNNLSGGV
                     NGYMLLYNGQRVINAKRQLNTVEAQTRQVLSSRALILVNNVLLKYYDVVRQQSYAKTL
                     QASIDAQKQQLAIVQAQQSVGLANNADLFQSQVDLNTQVQNLQAQQLVIDQDKTDLLT
                     LMTLNPDSAIVIEDTILIDRDIQLPTILSAVRDANPDVIAANLQITVNQYIENQVSAL
                     RYPSLTVNGGYNYSRTNNSSASQYSPLFSLNYGPYVGVTLGIPIFNGNIYKKQQQVAG
                     INIKNARAVRDTLVLGYTSAAVKSYQAYTSNLRQVATAQATYDLSRKLLDLVMQRFQL
                     KQATIVDVKNAQQSFENAGFLLVNVSYAAKASEITLRRYANQLVN"
     gene            complement(8212..>8643)
                     /locus_tag="VGQ51_13710"
     CDS             complement(8212..>8643)
                     /locus_tag="VGQ51_13710"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV9037684.1"
                     /translation="EISFVDHLEESELRRPDLQGPVTPLVIQCIEQLIQYFQGQRRVF
                     DLPISQDGTSFQQRVWNELMAIPFGKTLSYLELSRRLGDTKAIRAAASANGRNNVVIV
                     VPCHRVIGSKRDLVGYGGGLWRKKWLLEHENKIMYGVQTLF"
BASE COUNT         2019 a         2428 c         2419 g         1777 t
ORIGIN      
        1 ttcgtctgga tcgcgaacaa tatacaatac gatgtggtca atatgttcgc gctgaacttt
       61 tatgaagacc cggccgaaaa gatcgccagg cctctgaaaa cgcggaaagg catctgtgag
      121 aactatgcat cccttttcac cgcgatctgt aaccgggtgg gcatccggag cctggtcgtg
      181 gaaggctata ccaaagagcg gggcttcgtg ggctatatac cacatgcctg gagcgcagcc
      241 tttctcgacg gcaaatggtg gcttttcgat cccacctggg gatctggtta cgtggataac
      301 ggtaaattcg tcaggaagat gaatgaatcc tatttcaagg cggcgccgga gaattttata
      361 gccacacata tgcccttcga ctacctatgg gaattcctgt actaccccgt gaccaccggg
      421 gatttcttca atgcaaagac cggccaggac aagactaaac cacggttcga ttttcccgac
      481 tctatccgtg cttatgaggc gatgccaaaa gaggaacagg aggaggcggc ggccgaccgg
      541 atcgaaaaga acggcgtacg caattcgtct gcggcagata tggtccggca tctgcgggtg
      601 gatgtcgata attatcaccg gcaacaggag aatgatcgcc agaacaggat catcgattcg
      661 ttcaatatgg ccacgaggac ctataatcaa tcggtgaatc agttcaattt attcataaat
      721 tattataacg tgcaattcaa gccgccgaag cccgatgcgg agatacaaca gatgctggat
      781 accgcggctc atcagatgca ggaggcgaag acgctggcga cgaccattgt ccttacgccg
      841 gcagacagcc gggtagcaaa gcctttggat cagctgaaag aaggcattgc gacgcaggag
      901 gctcatatga aggaaatgga agaatggctc gtgaaatatt tgagcaagag caagctcggc
      961 cggagatcca tgttctataa aatcacctgg ttcggcatac ccgtgaatta atgtcaatgt
     1021 catggaaaaa aaagaaatac gcctggcgta cgaagaatac gcgtcggacc gggagctgac
     1081 ggcctccgat ggcggtttgc ttgagacggc ccgcaaggcc accggggatg cctatgcgcc
     1141 ttattcgcgt tttcgcgtcg gggcggcggc gcggttggcc aatggagagg tgatcacggg
     1201 cagcaaccag gagaatgcct cttttcctgc cggcatctgc gcggaacgga ccctgttatc
     1261 cgctgcggcg tctcgctttc ccggcgtccc catcgaaacc atcgcgatca gctatgtcaa
     1321 cgggcaggga cccagcgacc gccccatttc tccctgcggc atctgccggc aatccctgca
     1381 ggagatggaa caacggacgg gtcgccccat ccggctggtg ctggggggaa tggaaggacg
     1441 agtcatgatc ctcccgcagg ccgggcagct gttgcccctg gcctttaccg cggatgaatt
     1501 gggttgagga caggcgaacc atttacggcg gctcagccgg attgccgaaa agaaagctcc
     1561 acatccgtct ccggatagcc tgtgcaggtg aggacgagcc cttcacggac atccttatcg
     1621 gtcagcacct cgttattgct catttttacg ctgccgctga tacaccgcgc tacacaggtc
     1681 gagcaccggc cggcgcgaca gctaaagggc agatggagtc cctccttctc agcggcctga
     1741 aggatggtat cgggccattt catttcaaaa cggtggtgga cattggcgcc gtcatggatc
     1801 accacgatcc tgggcgtctt gtcgaagacg ggtggagggg cggggatatg ttctaccgtg
     1861 aaatgttctt tcctgatatg cctttcgggg atgcccaggg tgcgaagggt gaactgcgcc
     1921 atccggataa aggccggcgg tccgcagata tagaactgcg tcccgggtaa tgcctcccgc
     1981 ggcagaagac cgaacaacca ttcaccgagc gaccagttat tgagacgacc atcgcggatc
     2041 gtcagcagat cgatccactg gaaaccatca cgcgccgcca gcgtttggag gctgtcgtaa
     2101 aagggagcgc tttcccgatc gtgaaactgg ctgatcagca cgacccgcgc atcctccgcc
     2161 agtgcctgtt tcagcaagga gaatatcggt acgatgccgc tgcccgcccc caaaaagaaa
     2221 tgcacagccg ccgtctcccc gagaaaaaac cggccggaag gcggaagact ggtcagggta
     2281 tcgcccacgc gaagatgatc gagcaaaaac cgcgatatct caccattcgg cacgcgtttg
     2341 acggtgatag ccggggtcgg atcgataccc ggcgtcgtac aaatcgaata gctgcgcctg
     2401 atatcatgcc ccttcggggt gaaaagcagg gtgaggaact gtccggcggt ataagagaag
     2461 gggccgccgt cttcccggtc aagaaagatg gtcttggtgt ctacggcttc cgccctgata
     2521 tccaatacgc gtagcgtcca tattaccatg ctattacctt tttccgtcgc tctaaaataa
     2581 cgataattgg gggtctggga ggatgatcgg gggacttaag cccttaattc tttttatctt
     2641 tgcagacttt gtttcaaaaa tcgctctgaa tggcctgttc ttttaagcta acctctctcg
     2701 ccggattatc ctgcgcgctc ctgctcacct caatgggcct tggttccctg tcttcctgta
     2761 aacataccga tgcggagtca tccaccctcg ggaaaaagca agccgaggcg accatcgtgg
     2821 atgtgctggt tgcggcgccc cataccctga ccaaccaggt agaagccaat ggtaccgtcg
     2881 tagccaacga atacgtagag ctgcatcccg agatcagcgg gcgtatcgtc tatctgaatg
     2941 ttccggaggg ggccagggtg agcaagggca ccgtcatcgc aaggatcaat gacgccgatc
     3001 ttcaggccac cctcgagaaa tcgaaggccc agctcgatat gtaccaaaaa acggtagaac
     3061 gcgagcacaa acttctggag atcaatggta tcaatcaggc cgattacgac ctcgccgtaa
     3121 gcaatgtcaa cgctacgaag gccgatatcg attataccca ggctcagctg gacaagacgg
     3181 tcatccgggc gcctttcgac ggcgtggtag gtcttcgcca ggtaagtccc ggggcttatg
     3241 tgacgtctgc caatatcatc gctaccgtcc agcagatgga gaagataaaa gtggatttta
     3301 ccgtgcccga agagtattcg gccgtcatcc ataagggtgg cgtcgtagac atcgagctgg
     3361 atgccatcaa caacacgcat cgcaaggggc tgatcatcgc caccgaaccc cagatcaatc
     3421 tttccagccg taacctgaag gtccgggcgc tgcttgcgga tggttcggcc catcccggcg
     3481 ccttcgttaa ggtcttcatc aatacgggcg tggataaaaa agcgatcctt gttcccacca
     3541 attgcatcat tccggaggac aggaccaacc aggtcatggt ggtgaaggat ggtctggcga
     3601 aattggtggc ggtcggcacg ggggaaagac aatcgggcaa tgtggccatc accagcggcc
     3661 tcaatgccgg cgatacggtc attgttacgg gtgtcctgtt tgcgcgtccc ggaactcccg
     3721 tcaccattcg cgctgtcaag acgctcgata atgcttcacc ggcctccaac tagatctaat
     3781 gaatatttcc gaactctctc tcaaacgacc cgttctggcg acagtgatga acctgatgct
     3841 catcctgttc ggcgtagtgg gttataattt cctggcggta agggactatc cggccatcga
     3901 tccgccgatc atcacggtac agaccaatta caccggcgcc aatcccgatc tcatcgagaa
     3961 ccagatcacc gagccgctgg agaaaaatat caacggcatt cccgggatcc ggaccatctc
     4021 ttccacgagc aatctcggca gcagctatat cacggtcgaa ttcaacctgg gcgtcgacct
     4081 cgaggccgcc gcgagcgacg tacgcgataa agtgggttcg gcggcaagat ccctgcccct
     4141 cgatatcgat gcgccgccga tcgtgaccaa ggccgacgca aacagcgatt tcatcctcat
     4201 cctggccgtt caaagccgca cgaagagcct gatggaactc agcgattacg cggagaacgt
     4261 gctccagcaa cagctgcaga cgatcgatca ggttagcgca gtctacgttt tcggagaaaa
     4321 gacctactcc atgcgcatct ggctcgacca ggacaagatg aatgcgtatg gcgtcgctta
     4381 cagcgacatc gccaccgcac tgaacaacga gaatgtcgag cttccgccgg gcaagatcta
     4441 tggcaacaac accgagctca ccatcaaaac gctgggccgt ctgacgacgg aaaaggattt
     4501 ccgggacgtc atcctgcgcg aggacagcag cggcatcgtc cgtttgagcg acgtggcaag
     4561 agtggaaata ggcccgcaaa acccggagca gagctggaaa tacaacggcg tgaacgcggt
     4621 cggtctggcg atcatcccgc aacccggcgc caataatatc gccatcgccg atgaattcaa
     4681 ccggcggctg gacgagatca aaaaagccaa caaatcggat ctcgaggtca acgtcctcgt
     4741 cgacaatacg cgaacgatcc gtcaatcgct gtcggaggtg aaggaaacgc tgctcatctc
     4801 gctttcgctc gtggtcctgg tcatcttctt tttcttccgc aactggatga tcgctttgcg
     4861 tcctctgatc gatatcccga tctcgctgat cgccaccttc ttcatcatgt acctggccgg
     4921 gttcacgatc aatatcctga cgctgctggg tatcgtcctg gcaaccggat tggtcgtgga
     4981 tgacgggatc gtcgtgaccg agaacatctt ccggaagctg gaacagggga tgccgatccg
     5041 aaaggcggcg ctggaaggca gcaaggagat cttttttgcc gtgatctcca cttcgttgac
     5101 cctggctgtc gtattcctgc cggtgatctt tttgcagggc ttcgtaggaa ggctcttccg
     5161 cgaattcggc gtcgtgctgg ccgccgcggt gctgatctcg tctttcgtct cgctgaccat
     5221 cacccccgtg ctgaacgtct acctcacccg taagaacagc gggcacgggc ggttctacga
     5281 aaggaccgaa ccgttcttca cgggcatgga gaacggctac aggcggctgt tgacggcctt
     5341 catgaaggtg cgccggatgg catgggtgat cattgcggtg tgctgcgcca tcatcgtgtt
     5401 cgtgatgagg cacattcaaa gcgagatcgc gccgatggaa gaccgcaaca gcatccgttt
     5461 caccgtaacc ttgcccgaag gttccagcta ttcctataca caggccgtca ccgaccagat
     5521 atccaactac ctgctcgatt cggtaccaga gagagaattc gtttttgcaa ggacgccgac
     5581 caacaacagc tcgaatgcct cccaacccag gatcggcctg gtggagccct ggatgcgcaa
     5641 gcgttcgcag aatgagatcg ccaacgatct tcagcgtaag ctctcgcgat tcaacgatgc
     5701 gcgcattttc gccatccagg aacagaccat cgccgtgggc tcgggctcga agaccagcct
     5761 gccggtgcag ttcgtgctgg agaaccagga cctcgacaag atgaaagcga tattgccggc
     5821 cttcctggat tcctgtcgca aggataagac gttctccaac gtggacgtga acctgaaatt
     5881 caacaagcct gagctgcagg tcaccgtcga ccggatgaag gtgcgcgacc tcggtctttc
     5941 gaccagcgaa gtcatctccg cgatgcaggc ggcattcagc ggcggaagac tggcctattt
     6001 catcatgaat ggctaccagt attatgtcat tgcgcaagtg gagcgacagg accggaacga
     6061 gccggccgac atcagcaaga tatatgtgcg caataagaac ggcgacccga taccgctgag
     6121 cgctgtcctg catgtcgagt caaacagcag tccgtcgaca ctgtaccatt tcaaccgcta
     6181 taaggcagcg accatttcgg cttcgctggc ggagggaaag accgtggggg acggtatcaa
     6241 gaccatgcaa cggatcgccg acaagctcct cgatgccagt ttccagacgg ccctgtcggg
     6301 agcctcgcgc gactatgcgg aaagttcttc gaatatcatt tttgcgtttg tgctggcgct
     6361 ggtgcttatc tacctggtgc tgtccgcgca gttcgaaagc ttcagggacc cgctcgtgat
     6421 catgctgacc gtccccctgg cgctggccgg tgcgttactc agtctttggg tctttcatca
     6481 aacgctgaat atcttttcgg agatcgggat gatcatgctg atcggacttg tgacgaagaa
     6541 tggcatcctg atcgtggagt tcacgaatca gaagcgggag aaaggcatgc caaaacgcga
     6601 ggcagtgatc gaggcggcgt cgcatcggct gaggcctatc ctgatgactt cgctggcgac
     6661 ctcgctcggg gccctgccca ttgcgctcag tctcggggcg gcggcttcca gccgtatccc
     6721 tttgggtatc gtagtggtgg gtgggatcct gttctcgctg atcctgacct tattcgtaat
     6781 tccggccgtg tataccttca tttcgggccg acataagaac cacccgacct caccggtaga
     6841 ggaattgcaa gagagcgaac cgatagaaca aatatgagaa aactatattc ggtttggacg
     6901 gcgttgacgc tgtcgaccat gatcctgcag gcacaaccac tgactcgcga cgaagcggtc
     6961 cgtatcgcgc tgaagaacaa cctgggcatc gagctggcca ggaacgccgt cgcgatcgcc
     7021 tcgatcaaca acagctatgg catcgccgga ggactgcccg tagtgaccgg tacgatatcc
     7081 gaccaggaga attcacccag cttcaggcag gtgtattcca atgccgcgag taacaaggcc
     7141 agcaacaacg ccttttccaa caatctgtcg ggcggcgtca acggttatat gctgctctac
     7201 aacggccagc gggtgatcaa tgccaaacgg cagctgaata ccgtcgaagc ccaaacgcgg
     7261 caggtgctga gctcgcgggc gctgatactg gtcaacaacg tattgctgaa atattatgac
     7321 gtcgtacggc agcagagcta tgcgaagacc ctgcaggcct ccatcgacgc gcagaaacaa
     7381 cagctcgcga tcgtacaggc gcagcagagc gtgggtctgg ccaacaacgc ggaccttttt
     7441 cagtcgcagg tcgatctgaa cacacaggta cagaacctcc aggcgcagca gctggtcatc
     7501 gaccaggaca agaccgatct gctaacactg atgacgctga accctgattc ggccatcgtc
     7561 atcgaggata cgatcctgat cgaccgcgac atccagctgc cgacgatcct cagcgccgtt
     7621 cgggacgcca acccggacgt cattgccgcc aatttgcaga tcacggtgaa ccagtatatc
     7681 gaaaatcagg tgagcgcatt gcgttatcct tcgctgaccg tgaacggagg ctacaactac
     7741 agccgcacga ataattcgag cgcttcgcaa tacagcccgc tgttcagcct gaattacggg
     7801 ccctatgtcg gcgtgacgct gggcataccg atcttcaacg ggaatatcta taagaaacaa
     7861 caacaggtag ccggtatcaa tattaagaat gcccgggcgg tccgggatac cctggtattg
     7921 ggctacactt ccgccgcggt caagagctac caggcctata caagcaacct ccgtcaggtg
     7981 gcgacggcgc aggccacata tgacctttcg cgtaagctgc tcgatctggt gatgcagcgg
     8041 ttccagctca aacaggccac gatcgtggat gtgaagaatg cccagcaaag cttcgagaat
     8101 gcgggattcc tgctggtcaa tgtgagctat gcggcgaagg cttcggagat tactttacgc
     8161 cggtacgcga atcagctggt aaattaaacc tgcccgcacc acggtgcccg gtcagaataa
     8221 cgtctgcacg ccgtacatga tcttattctc atgctcgagc agccatttct ttctccaaag
     8281 accaccgcca taacccacca gatccctttt agacccgatg acgcgatgac aggggactac
     8341 gatgacgaca ttgttccgtc cgttggctga ggcggccgcg cgtatggcct tggtatcgcc
     8401 gagccggcgc gatagctcga gatagctgag cgtcttgcca aacgggatag ccatgagctc
     8461 gttccatacc cgttgctgga aagacgtgcc gtcctgtgag atcgggagat cgaagacgcg
     8521 gcgctggccc tgaaaatact ggatcagctg ttcgatgcat tggatgacca gcggggtgac
     8581 gggcccctgg agatccggcc ggcgaagttc ggattcctcg agatgatcga cgaaactgat
     8641 ctc
//
LOCUS       DASYSV010000446         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_862220, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000446 DASYSV010000000
VERSION     DASYSV010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862220"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="VGQ51_13715"
     CDS             complement(<1..1510)
                     /locus_tag="VGQ51_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037685.1"
                     /translation="MYRFLLAIGLLLCLRSKAQLPTDVLRSSWTMPGGTAREQAIGGA
                     MGSLGGDISAASVNPAGLGLYKTSELVLTPGWRFLIDKGNYLGTNTKAPALNHFATGA
                     SGVVFAYGSGDPGVSNAFAISVNRMADFNSHVSYSAINNYSSFSEQYVEEFAASGLDI
                     NSGIASPSLDYGTRMALYTSLVDTATINGTLQVIGQPQKAGRLLQQNDLRTSGGITEI
                     QLSLATSRQDKWYIGGSLGIPIFDYTRNQTFTESDATSNSNNDFESFSYRETYTTKGT
                     GINLKLGAIFSPSTPWRIGFAIHTPSVYALTDHISASMITRTENYTSLPQVSISSDSL
                     DQLTGLSPQPNSVTYDLYTPWHFLLSGSYIFGSGQAETKKQKGFITADLEYITTHSPH
                     YSVPSDEDQGSGAGDYYDQVNQAIKQLYKGTYSARIGGEMKFNTLLVRAGGAYYSNPY
                     SQSGLKVDRLFLSGGLGYRTKAFFADLTYVMRLSNDIDVPYYLADKNNSYASL"
     gene            complement(1598..2404)
                     /locus_tag="VGQ51_13720"
     CDS             complement(1598..2404)
                     /locus_tag="VGQ51_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037686.1"
                     /translation="MTRVYTLLAVAIVVGMSSCSSSRNAQNTDDVYYSNGGSKGATTA
                     GGANQGDYYSTSPSDNYVRMAMTDPRFSTFEDYNAYDSYYYSPASASMGYGFGYPAFG
                     FGYPMYGMGMGFSYGLGFGMGMGIYDPYMAWNSYFMWNSWYNPYFYNPCYGVPVMMVG
                     HNAAPTAIYTNLRPFNSVAYRNGLYARGGTSVRNNNRYYRPGMTGTTSYNNRVNHVNS
                     TARPVNQTYYRPVNNSNGGFRPGSFSAPTRSYSPPMGGGGGGGGIRPGRH"
     gene            2526..>3439
                     /locus_tag="VGQ51_13725"
     CDS             2526..>3439
                     /locus_tag="VGQ51_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEV9037687.1"
                     /translation="MSKEITSRAADYSQWYNDLVIKGGLADHSEVRGCMVIKPYGFAL
                     WERMRDQLDRMFKDTGHVNAYFPLFIPKSFLSREAAHVEGFAKECAVVTHYRLMNDPN
                     GGGVVVDPTAKLEEELIIRPTSETIIWNTYRDWIRSWRDLPLLINQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATAVEAIAETRQMLDVYATFAEEYMALPVIKGVKSESERFA
                     GAVDTYCIEALMQDGKALQAGTSHFLGQNFAKAFDVRYSDRNNQLDYVWATSWGVSTR
                     LIGGLVMAHSDDDGLILPPRIAPLQAVIV"
BASE COUNT          764 a          817 c         1071 g          787 t
ORIGIN      
        1 tcagcgaagc ataggaattg ttcttatcgg cgagatagta gggaacatcg atatcgttgc
       61 tgagccgcat gacgtaggtg aggtcggcga aaaaggcttt ggtgcgatag cccagaccac
      121 cgctgaggaa gagcctgtcg accttcagac cggattgaga gtatggatta ctgtagtatg
      181 cgccgcccgc cctgacaagt aaggtgttga atttcatctc tccgccgatg cgggcgctgt
      241 aggtaccttt gtacagttgt ttgatggcct ggttgacctg atcgtaataa tcgccggcgc
      301 ccgagccctg gtcttcatcg ctgggaacgg aataatgggg gctatgggtt gtgatgtatt
      361 cgagatcggc ggtgatgaaa cctttttgtt ttttggtttc ggcctgaccg ctgccgaaaa
      421 tatacgagcc gctgagaaga aaatgccagg gagtataaag atcgtaggta acggaattgg
      481 gttgcggcga caagccggtc agttgatcca gcgagtcgga gctgatggac acctgcggga
      541 gagaggtata gttctcggta cgggtgatca tcgaggcgct gatatggtcg gtcagtgcat
      601 aaacgctggg ggtatggatg gcgaatccga tgcgccaggg ggtcgaaggg ctgaagatgg
      661 cacccagctt cagattgatc cccgtgcctt tggtagtata ggtctcgcga taagaaaagg
      721 attcaaaatc gttgttggaa ttggaggttg catcggattc ggtaaaggtc tggttgcggg
      781 tatagtcgaa gatgggaata cccaggcttc cgccgatgta ccatttgtcc tgccgggaag
      841 tagcgagaga cagctggatc tcggtgattc caccgctggt gcggagatcg ttttgctgga
      901 gcagccgccc cgccttctgc ggctggccga tcacctgcag cgtaccgttg atagtggccg
      961 tatccaccaa tgaagtataa agcgccatgc gtgtgccgta gtccagcgaa gggctcgcga
     1021 tgccgctgtt gatatcgaga cccgaggcgg cgaattcctc gacatactgt tcggaaaaag
     1081 agctgtagtt attgatcgcc gaataggaga catggctgtt gaaatctgcc atccggttga
     1141 ccgaaatggc gaaggcgttg ctgacgccgg gatctccgct gccgtatgcg aaaacgacgc
     1201 cgctggcgcc cgtcgcgaaa tggttgaggg cgggggcctt tgtgttcgtg cccaggtaat
     1261 tgcctttatc gatgaggaaa cgccagcctg gagtgaggac cagctcgctg gttttataga
     1321 ggccgagacc ggcaggattg acggaggccg cggagatatc tccgccgagt gaccccatgg
     1381 cccctccgat ggcttgttcg cgtgccgtcc ccccgggcat tgtccaggac gagcggagga
     1441 cgtcggtcgg taattgcgct ttggatcgga gacacaggag tagccctatg gccaggagaa
     1501 atctatacat agcaatggtg ttgagcctcg ggcctcatct gttagaaaag cgcccgggtg
     1561 gttagctgtc tgaaggtagg tgctccgttg aatgttctta gtgtctgcct ggtctgattc
     1621 cgccgccgcc acctccaccg cccatggggg gtgagtagct acgggtaggt gcgctgaaag
     1681 agccgggtct gaaaccgccg ttggagttgt tgacagggcg gtaataggtc tgattcacgg
     1741 gtcttgccgt gctgtttaca tggtttacac ggttgttata ggacgtggtc cctgtcattc
     1801 ccggacggta gtaccggttg ttgttgcgga cgctggtgcc gcccctggca tagagcccgt
     1861 tgcggtaggc gaccgaattg aacggccgga gattggtata gatcgccgtg ggagcggcat
     1921 tatgtccgac catcatcacc gggacgccgt aacaaggatt gtagaaataa ggattgtacc
     1981 agctgttcca catgaagtag ctgttccagg ccatatacgg gtcatagata cccatgccca
     2041 taccgaaacc gagaccatag gagaagccca ttcccattcc gtacatcgga tatccgaaac
     2101 caaaggcggg ataaccaaaa ccataaccca tggaagccga ggccggggaa tagtaatagg
     2161 aatcgtatgc gttgtaatct tcgaaggtgg aaaagcgggg gtccgtcatc gccatgcgta
     2221 catagttgtc gctgggagaa gtggagtagt agtcaccctg attggcgcca cctgccgtgg
     2281 tggcaccctt cgagccgccg ttcgaatagt agacatcgtc tgtattttgt gcgttgcggg
     2341 aggaagagca gctgctcatt ccaacgacta tcgcaacggc aagcagggta tatactcgtg
     2401 tcatgatcgt agtttttagc attgaagttg ttacatttgc aactccctgt taaagttaca
     2461 ataaataacg agccttaaca gcaaaaagtt aacattttat tgttaaaagc tgattaataa
     2521 attatatgag caaggaaatc acgagccggg cagctgatta ttcgcagtgg tataacgacc
     2581 tggtgatcaa gggcggtttg gcagatcatt cggaggtccg cggttgcatg gtcatcaaac
     2641 cctatggatt tgccctttgg gagcggatgc gcgaccagct tgacaggatg ttcaaggaca
     2701 ccgggcatgt caacgcctat tttcctttgt tcattcccaa gagctttctc agcagggaag
     2761 ctgcccatgt ggaaggtttc gccaaggagt gcgccgtcgt gacgcattac cggctgatga
     2821 acgacccgaa cggcggtggg gtggtcgtcg atccgacggc caaactggaa gaagagctga
     2881 tcatccgtcc caccagcgag accatcatct ggaataccta tcgtgactgg atccgcagct
     2941 ggagggatct gcccctgctg atcaatcagt gggcgaatgt cgttcgctgg gagatgcgga
     3001 cgcgtctatt tctgcgtacg gccgaattcc tgtggcagga ggggcatacc gcccatgcca
     3061 ccgcggtgga ggccattgcc gagacgaggc agatgctgga tgtctatgct accttcgcgg
     3121 aagaatatat ggccctgcct gttatcaagg gcgtgaagag cgagagcgag cggttcgcgg
     3181 gtgcagtgga tacctattgt atcgaagccc tcatgcagga cgggaaggcg ttgcaggcgg
     3241 gcacctcgca tttccttggc cagaattttg caaaagcgtt cgatgtcagg tactccgaca
     3301 ggaacaacca gctggattat gtctgggcga cgagctgggg ggtaagcacg cgtctgatcg
     3361 ggggactggt catggcacat agcgacgacg acgggctcat attgccgcca cgtattgcgc
     3421 cgctgcaggc tgttatcgt
//
LOCUS       DASYSV010000447         6750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_603194, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000447 DASYSV010000000
VERSION     DASYSV010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603194"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VGQ51_13730"
     CDS             complement(<1..776)
                     /locus_tag="VGQ51_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037688.1"
                     /translation="MYSFQLENFEAWLSGLAGQLNASVNDRVLTIPDELGSGTILAQN
                     INPQLSYAVMNFKLDSDLEFHRESGAAGFVISFNMVEVQKELLVGHRQHIVSDKRPFR
                     NDIFLTDARDSQFLRISAGSNVRRLLILCSNELALQYLPQEIFEKIAAFARENSISDH
                     PLFINLSHRTMLKELFELKANDPLGSIRRLSGIIHLIEKFLHSFLRQEQAALPRTVKK
                     SDLESMQHIEHILSSRLEGFPSLESLAHEVFMSTSKLKNL"
     gene            complement(1177..2103)
                     /locus_tag="VGQ51_13735"
     CDS             complement(1177..2103)
                     /locus_tag="VGQ51_13735"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV9037689.1"
                     /translation="MTRFSCQLHDFRHWLTDFAKLLGQPICNGRLELPPYLGEGYIRA
                     GHLPAGVSYVIMDFSLTENISLIRLPSREAGLCLFFHPAPLLTTTRKEEETVLSPDYV
                     IKRVGLFFPALFLHRHIRRDILDCLFRYTENHHVPDAKDPFSFEYKPMLEDIFSIEDD
                     SALLHLTLHNRILLLAEKFLHSFLSRATYTPQGKTWVRSKEKDLQALKNIVQILSDSQ
                     LSQFPSIDTLSRTAMMSSTKLKTRFKQIYGMKLYEFYNRNRLEQAREMLRTGNYTVKQ
                     VGINIGFSNLSNFAKAFRKEFGLLPKEVLKNR"
     gene            complement(2227..4818)
                     /gene="mutS"
                     /locus_tag="VGQ51_13740"
     CDS             complement(2227..4818)
                     /gene="mutS"
                     /locus_tag="VGQ51_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV9037690.1"
                     /translation="MAKSSSDTPLMQQHRAIKQRYPDAILLFRVGDFYETFGEDAIVA
                     SKILGITLTKRNNGAAASAELAGFPHHALDSYLHKLVKAGHRVAVCDQLEDPKMVKGL
                     VKRGVTELVTPGVALNDRLLEGASNNFLAAIYFPDNDTTLGLSFLDISTGEFFIAEGD
                     TEYADKLLQTLKPAEIVFQRSFQRHFKEAFGSRWFTYTLEAWLFEEAFATEILLKHFQ
                     THSLKGFGIDKMNAGIIAAGAVLHYLKDTEHPQLQHITSIQRIDREDYLWMDKFTIRN
                     LELMGGPVSLLGVLDNTVSPMGARLLKRWMLMPLKDARQVRQRLDAVEFFIKDPGLRS
                     TLSQHIQACGDIERLVSRIPSRKIGPRECWQLARGLEHIAQIKEAAKAFPEDWLGQSA
                     ERLDPCPAVLEKIGSTLKENPPAAAGKGDTIRQGVNADLDDLRDIATGGKEYLARLQQ
                     QEAERSGITSLKIGFNNVFGYYLEVTNAHKNKVPQDWMRKQTLANAERYITPELKEYE
                     EKIIGAEEKILTLEARLYEELLVFLQEAIAPIQTNGHLLAELDCLLCFSANALQYTYK
                     KPQLHDGRELDLKASRHPVIERQLTSGEAYIPNDVLLDPATQQIIILTGPNMSGKSAL
                     LRQTALITLMTHMGSFVPCESARIPLTDKIFTRVGASDNLSGGESTFMVEMNETASII
                     NNVSPDSLVLLDEIGRGTSTYDGISIAWSIAEYLHNSPNAPKTLFATHYHELNELEDK
                     LSRVRNYHITNKEVGNKVIFLRKLAPGGSIHSFGIHVARMAGMPHTLLRRAEEILHQL
                     ETKHVGTSPGDSLRNIPAPKMQLSIFDEHTEAFQQIRRLLEDIDINRLTPVEALLKLQ
                     EIKNTLK"
     gene            complement(4895..6469)
                     /locus_tag="VGQ51_13745"
     CDS             complement(4895..6469)
                     /locus_tag="VGQ51_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV9037691.1"
                     /translation="MRKHSVLLVAGLLLLATTTKAQDEKIDLATIQKIRNEGLQHSHV
                     MDIAFHLTDASGNRLTASPGFYRAANWAKQQLAEWGLANAKLDPWGDFGKGWELQKSY
                     LALTAPYYKPLIAYPKAWCGGTDGPRSAAVILISATDSAGLDRYKGQLKGKILIPEID
                     AVYQQSFKPDADRWTDEQLDSMSKAVIRPVDTAELRRRRELFRRGGNQAFLNTLHRMA
                     EQEGAIAILSMSPRGHDGTLFVQGGGRYQLNDQPNFLDMVVTLEDYTSIVRLLKANTP
                     VALDIDVKTRFITDDTKGYNVIAEIPGTDRNLKDEVVMLGGHLDSWHSATGATDNAAG
                     CTVMMEAIRILKTLGIQPRRTIRIALWSGEEEGLLGSRGYVKKTFADPATMQLLPAHE
                     KFDAYFNIDNGTGKVRGIYLQGNEACRSIFTQWLQPFHDLGATTVTINNTGGTDHQSF
                     DAVGLPGFQFIQDEIEYSTRTHHTNMDSYEHLRQDDLSQIATIVAAFVYNTAQRDTRL
                     PRKPLPAPRPAGRGGF"
     gene            6508..>6750
                     /locus_tag="VGQ51_13750"
     CDS             6508..>6750
                     /locus_tag="VGQ51_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEV9037692.1"
                     /translation="MRKLGMDELHRLSVNEFRQSEKMPVVVVLDNIRSMHNVGSVFRT
                     ADAFLLSGIYLCGYTPQPPHRDIHKTALGATETVEWK"
BASE COUNT         1353 a         1529 c         2190 g         1678 t
ORIGIN      
        1 aagaggttct tcagcttgct tgtgctcatg aagacctcat gggcgaggga ctccagcgat
       61 gggaatcctt cgagacggct gctaaggatg tgttcgatat gctgcatgct ttccagatcg
      121 ctctttttga cggtgcgggg aagtgcggcc tgttcctggc ggagaaagga atgaaggaat
      181 ttctcgatga gatggatgat cccggagagt cttcggatcg accccagcgg atcgttcgcc
      241 ttgagctcga acagctcttt cagcatggtc cgatgggaga gattgatgaa cagcggatga
      301 tcgctgatac tgttctccct ggcgaaggcg gcgatctttt cgaagatctc ctgcgggagg
      361 tattgcagcg ccagctcgtt cgagcataag atgagcagtc tgcggacgtt gcttcctgca
      421 gagatgcgaa gaaattggct gtcacgggca tcggtcagga agatatcatt ccggaaagga
      481 cgtttgtcgg agacgatatg ctgacggtga cccaccagga gctctttctg gacttctacc
      541 atattaaaag agatgacgaa tccggccgcg cccgattccc ggtggaattc cagatcggaa
      601 tcgagtttga agttcatgac cgcataagaa agttgcggat tgatattttg cgcgaggatg
      661 gttccggagc caagctcgtc cgggatggtc aggacacgat cgttgacgga tgcgttcagt
      721 tggccggcga ggccggaaag ccaagcttcg aagttttcca gttggaagga gtacattacc
      781 atattcacct gtttttacac gattaacaaa tatctctcaa tggcaatcag ttcagctcag
      841 gatattggag gcatgtttga ggtgccatgc aaaagtgcaa atttacggca aaatatggct
      901 catagaggct gcgggggctt cggctttggc tgtaatggct atttaacaaa actttcaggg
      961 ggattcgccc tgatttttta ggtatttcta tgtagtgcag gcggagggac ggtgcgttcg
     1021 gggttcgatg agtgcggagg gacgcgcgcg gctggtgcgt tgagggctcg aagagtgagg
     1081 agggacggcc gctacctgtg ccttgagggc tcgaagagtg gggagggacg gccgctgcct
     1141 gtgcgttgag gctcgaagag tgaggaggga cggccgctac ctgtttttta aaacctcttt
     1201 aggcagaagg ccaaattctt tgcggaaggc tttggcaaaa ttgctgaggt tggaaaatcc
     1261 gatattgatg cccacctgtt tgacggtgta attccccgtt ctgagcattt cgcgcgcttg
     1321 ttcgaggcgg ttacgattat agaactcgta cagcttcata ccgtagatct gtttgaaacg
     1381 cgttttcagt ttcgtggagc tcatcatcgc ggtgcgggac agggtgtcga tggacgggaa
     1441 ctgcgagagt tggctgtcac tgagtatttg cacgatattc ttcagcgcct ggaggtcttt
     1501 ttccttgctt ctgacccaag tctttccctg gggggtatag gtcgccctcg acagaaagga
     1561 atggaggaat ttttcggcga gcagcagtat acgattgtgc agcgtgaggt gcagcagggc
     1621 ggagtcgtcc tcgatactga agatgtcctc aagcataggc ttatattcga atgagaaagg
     1681 gtctttcgca tcggggacgt gatgattttc cgtgtaccgg aacaaacaat ccagaatgtc
     1741 tctccggata tgcctgtgga ggaagagtgc ggggaaaaag agtccgacgc gtttgataac
     1801 gtagtccggc gagagcactg tctcctcttc ttttcgggtg gtggtgagca gcggcgcggg
     1861 atggaagaaa agacaaagac cggcttcccg cgacggaaga cggatcaggg agatgttttc
     1921 tgtcaggcta aagtccatga tcacgtagga gactccggcg ggaagatgtc ctgcccggat
     1981 atacccttcc ccgaggtagg gaggtagttc gaggcggccg ttgcagatgg gttgaccgag
     2041 cagcttggcg aagtcggtca gccagtgacg gaaatcatgc aattgacagg aaaaccgggt
     2101 catacacttt tagcatttgt ttagcgtaaa ttaaccaagg aaccggtacg ctaaactttt
     2161 agggacataa gggactcagg ggaggacaaa agttcccttt ttcctgacgg aaaggggcag
     2221 ggtccgctat ttcagggtat tttttatttc ctggagcttg aggagcgctt ccacgggcgt
     2281 gagacggttg atatcgatat cttcgagcaa ccggcgtatt tgttggaaag cctcggtgtg
     2341 ttcgtcgaag atggatagct gcattttggg cgctggaata ttcctgagcg aatcgccggg
     2401 ggaggtgccg acgtgtttgg tttcgagctg gtgtaatatt tcttccgctc gcctgaggag
     2461 tgtgtggggc atgccggcca tccgggcgac atggataccg aagctgtgga tgcttccgcc
     2521 gggcgccagt ttgcggagga agatgacctt attgcctact tccttattgg tgatatgata
     2581 attacggaca cgggaaagct tgtcctccag ctcattgagc tcatgatagt gggtagcgaa
     2641 cagggttttg ggtgcgttgg gcgaattatg gaggtattcg gcgatactcc aggcgatgga
     2701 gatgccatcg taggtgctgg ttcccctgcc gatctcgtcc agcaggacaa ggctgtcggg
     2761 gctgacattg ttgatgatgc tggctgtttc attcatttca accatgaagg tggactcgcc
     2821 gccgctgaga ttatcggaag cgccaacgcg ggtgaagatc ttgtcggtga gggggatccg
     2881 ggcggactcg cagggtacga agctgcccat atgggtcatc agtgtgatga gcgcggtctg
     2941 gcggaggagg gcgcttttgc cactcatgtt cgggccggtg aggataatga tctgctgggt
     3001 ggcggggtcg agcagcacat cgttgggaat ataggcttcg cccgatgtca gttgtctttc
     3061 gatcaccgga tgacggctgg ctttgagatc cagctctcgg ccatcgtgca gctgcggttt
     3121 tttataagtg tattgaagcg cattggcgga aaagcagagc aggcaatcga gttcggccag
     3181 caggtgtccg ttggtctgta tcggggcgat cgcttcctga aggaagacca gcagctcttc
     3241 atagagccgc gcttcgaggg ttaatatctt ttcttctgcg ccgatgatct tttcctcgta
     3301 ttccttcaat tccggtgtta tatatcgttc ggcattggcg agcgtttgtt tacgcatcca
     3361 atcctgcggc accttgttct tgtgcgcgtt ggtcacctcg agatagtaac cgaagacatt
     3421 gttgaatccg atctttagcg acgtgatgcc ggagcgttcg gcttcctgct gttgcaggcg
     3481 ggcgagatat tctttgccgc cggttgcgat atcgcgtaga tcgtcaagat cggcatttac
     3541 gccctggcgg atggtatctc ccttgccggc tgcagcgggg ggattttctt tgagtgtgga
     3601 tccgatcttt tcgagaacgg cggggcaggg atcgagtctt tcggccgatt ggcccagcca
     3661 gtcttcggga aaggcttttg ccgcctcttt tatttgggcg atatgttcga ggcctctggc
     3721 cagctgccag cattcgcggg ggccgatctt tcgggacggt atcctggaga cgagccgttc
     3781 gatatccccg caggcctgga tatgctgaga cagggtggag cgcaggcccg gatcttttat
     3841 aaagaattcg acggcgtcga gccgctgacg gacctgtctg gcgtctttta atggcatcag
     3901 catccatctt ttgagcaggc gtgcacccat aggggagacg gtgttatcca ggacaccgag
     3961 caggcttacg gggccgccca tcagctccag atttcggatg gtgaacttgt ccatccacag
     4021 atagtcttcg cggtcgatgc gctggatgga ggtgatgtgt tgcagttgcg ggtgttcggt
     4081 gtccttaaga taatgaagta cggcgcctgc tgctatgata cctgcgttca ttttgtcgat
     4141 gccgaagcct ttcagcgaat gggtctgaaa gtgtttgagc aggatctccg tggcgaaggc
     4201 ttcttcgaac agccaggctt cgagcgtata ggtgaaccac cggctgccga acgcttcttt
     4261 gaaatgacgt tggaagctgc gctggaagac gatctcggcg ggtttgaggg tctgaaggag
     4321 cttgtccgcg tattcggtgt cgccttccgc gatgaagaat tcgccggtag agatgtcgag
     4381 aaaggacagg ccgagggttg tgtcgttgtc ggggaagtag atagcggcga ggaaattgtt
     4441 gcttgcacct tcgagcagac ggtcgttgag agctacacct ggcgtcacca attccgtgac
     4501 gccgcgtttg acgaggcctt tgaccatttt tgggtcttcg agctgatcgc agacagccac
     4561 gcggtggccg gctttgacga gcttgtgaag atatgagtcc agcgcatgat gggggaaacc
     4621 ggcgagctcg gcggatgcgg ccgcgccatt gtttcgtttg gtgagggtga tgccaaggat
     4681 ctttgaagct acgatcgcgt cctcgccgaa ggtctcatag aaatcgccga cgcggaataa
     4741 gagaatagca tcgggatatc gttgtttgat agcccgatgc tgttgcatga gaggggtgtc
     4801 ggagctgctt ttggccatga ggggcaaaaa taaggaagct cagacaaatg cggccggcat
     4861 tttagaagcc gcctggaagg tgcttagaag ccgtttagaa gccgccgcgg ccggcaggac
     4921 gcggcgccgg aagtggcttt ctgggcagcc tggtgtcgcg ttgcgccgtg ttatagacga
     4981 aggcggccac tatggttgct atctggctga gatcgtcctg tcgcaaatgt tcataggaat
     5041 ccatattggt atggtgggtg cgggtactgt attcgatctc gtcctggatg aactggaaac
     5101 cgggcagtcc aacggcgtcg aacgactggt gatcggtgcc gccggtatta ttgatggtca
     5161 ctgtcgtggc gccgaggtcg tggaagggtt gcagccattg ggtaaagatg gagcggcagg
     5221 cctcgttgcc ctgcagatag atgcccctga ccttaccggt gccgttgtcg atattgaaat
     5281 aggcgtcgaa tttttcatgg gcgggaagca gctgcatggt ggcggggtcg gcaaaggtct
     5341 tttttacata gccgcggctg ccgagcaggc cttcttcctc gccgctccat aatgcgatgc
     5401 ggatagtgcg ccggggttgg atgccgaggg ttttcagtat gcggatggct tccatcatca
     5461 cggtacagcc ggcggcgttg tcagtggcgc cggtggcgct atgccaggag tcaagatggc
     5521 cgccgagcat gacgacctcg tctttcagat tgcgatcggt gccggggatc tcggcgatga
     5581 cgttgtagcc tttggtatcg tcggtaatga atctcgtttt gacgtcgatg tcgagtgcga
     5641 ccggggtgtt cgctttcagg aggcggacga tggacgtata atcctcgagg gtgacgacca
     5701 tatcgaggaa attcggttgg tcgttcagtt gatagcggcc tccgccctgg acgaaaagtg
     5761 ttccgtcatg accgcgcggg gacatgctga ggatagcgat tgcaccttcc tgttctgcca
     5821 tgcggtgaag cgtattgagg aaggcctggt tgcctccgcg acggaagagt tcgcggcgac
     5881 ggcgaagctc tgcggtatct acagggcgga tgacggcctt cgacatagaa tcgagctgct
     5941 catccgtcca gcgatcggca tcgggtttga agctttgctg gtagactgcg tcgatctcgg
     6001 ggatgagtat ttttcctttc agttgacctt tataacggtc gaggccggcc gaatcggtgg
     6061 cgctgatgag gatcactgcg gcggatctgg gaccgtcggt ccctccgcac caggccttgg
     6121 gataggcgat caggggtttg taataggggg ctgtgagggc taaataggat ttctgcagct
     6181 cccatccctt tccgaaatcg ccccaggggt cgagttttgc gttggcgagt ccccattcgg
     6241 cgagttgttg tttagcccaa ttggctgccc gatagaaacc cggggaggcc gtcaggcggt
     6301 tgccgctggc gtcggtgagg tggaaggcga tgtccatgac gtgggaatgt tgcaaccctt
     6361 cattacggat cttttggata gtggcgaggt cgatcttttc atcctgggcc ttggtggtcg
     6421 tcgccaggag caggaggcct gcgacgagca ggacggagtg ttttctcatg agcgatgttt
     6481 ttctttaaag gtatggtatc tttgggcatg cgcaagttgg gaatggatga gctgcaccgg
     6541 ctatcggtga atgaattcag gcaatcggag aagatgccgg ttgtcgtggt gctggacaat
     6601 atccgcagca tgcataatgt ggggagtgtg ttccggactg ctgatgcgtt tttgctgagc
     6661 ggcatctatc tttgcggtta cacgccgcag ccgccgcacc gggatatcca taagacggcg
     6721 ttgggggcta cggagacggt ggaatggaaa
//
LOCUS       DASYSV010000448         3527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_603241, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000448 DASYSV010000000
VERSION     DASYSV010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603241"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VGQ51_13755"
     CDS             complement(<1..499)
                     /locus_tag="VGQ51_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV9037693.1"
                     /translation="MNKYDAIVIGSGISGGWAAKELTEKGLGVLMLERGHPYEHIRDY
                     KTASLDPWQFEHRGKVTQEQKKEHPYIARTWGATEAVIDSWAKGIDCPYTEVKPFNWW
                     RSYRVGGRSTLWGRQSYRWSDHYFEANGREGWAIDWPIRYNDLEPWYDHVERFIGVSG
                     SREGLP"
     gene            complement(496..2175)
                     /locus_tag="VGQ51_13760"
     CDS             complement(496..2175)
                     /locus_tag="VGQ51_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV9037694.1"
                     /translation="MADNTYDAIVIGSGISGGWAAKELTEKGLKVLMLERGRNIEHIK
                     DYPSANKEAWDFPHRGHATQEMKKDYPVLKRDYPLNEWNLDYWVKDSECPYTEVKRFD
                     WFRGYHVGGRSLMWGRQSYRWSDFHFEENGKQGIAVDWPVRYKDIEDWYTYVEKWAGI
                     SGSKEGIWHLPDSSFLPPYDMTCVEKDVAARIKDHYKGHRHMIIGRVANITVPHEGRP
                     GCQFRNKCWLGCPFGGYFSTQSSTLPAAMATKNLTLRPWSIVTRILYDKDKKKATGVE
                     VLDAETNKTYEYHAKIVFLNASTLNSAWVLMNSATDIWPDGLGSSSGELGHNLMDHHL
                     GVGASGIVEGYEDKYYFGRRANGIYIPRFRNLDGDKREYVRGFGYQGGGGREGYDHEV
                     PEYSIGKEYKEALTEPGPWRMNIGGFGEILPYHENKVTLDKTKKDKWGLNVLSIDCEL
                     KDNELKMRKDMEADAIEMLTIAGVKNIKSRSDNPFPGRGIHEMGTARMGRDPKTSVLN
                     SFNQVWDAKNVFVTDGSFMVSANCCNPSLTYMAFTARAADHAVKELKKGNI"
     gene            complement(2205..2894)
                     /locus_tag="VGQ51_13765"
     CDS             complement(2205..2894)
                     /locus_tag="VGQ51_13765"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV9037695.1"
                     /translation="MKRIGFSAIDLVGPADWPVLQAHGIFSSMCNGAEISLTKGWNHT
                     EYHPTLIKNYTEHIDLVSKAGYKNLICFSGNREGMDDETGLKNCVAGLKQVLSQAEQK
                     GVIVHMELLNSKINHKDQMCDNSKWGVELVKRIGSPNFKLLYDIYHMQIDEGDVIRTI
                     TDNHEYFGHYHTGGVPGRHEIDETQELFYPAIMRGIVATGYKGYVAQEFIPAKADKLK
                     SLEEGVVICDI"
     gene            2953..3192
                     /locus_tag="VGQ51_13770"
     CDS             2953..3192
                     /locus_tag="VGQ51_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037696.1"
                     /translation="MIVPFNFNFFGFLFAARAFAEALSEDSIPPVPSAAVPATMVLIA
                     VRREDLLDILLSTLKEESYKKIQFPAGGDTILMRL"
     gene            complement(3206..>3527)
                     /locus_tag="VGQ51_13775"
     CDS             complement(3206..>3527)
                     /locus_tag="VGQ51_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037697.1"
                     /translation="WPTMLGFVSEYLPKTGALGISLMGGAGMFSTSLFIPIMGGWFDH
                     FKSAAIAGGATAVDADHLAGSQTLMKVVILPVILIVVFSLIYIVRRKTYAAHKQQLHT
                     GAAG"
BASE COUNT          781 a         1108 c          755 g          883 t
ORIGIN      
        1 gaggcaaccc ttccctgctg ccactaactc cgatgaaccg ctccacatga tcataccagg
       61 gctcgagatc gttataacgg atcggccagt cgatcgccca gccttcccgt ccattagctt
      121 cgaaataatg atcgctccaa cggtagctct gcctgcccca caaggtcgaa cggccgccaa
      181 cccgataaga ccgccaccag ttaaaaggtt tcacctccgt ataagggcaa tcgataccct
      241 tcgcccagct gtcgatcacc gcttccgtgg cgccccaggt cctggcgata tacggatgtt
      301 ccttcttctg ctcttgcgta accttgcccc gatgctcgaa ctgccaggga tccaggctcg
      361 ccgttttata gtccctgata tgctcgtatg gatgtccccg ctcgagcatc agcacgccca
      421 gccccttttc cgtcagctcc ttcgcggccc agccgccgct gatcccggaa ccaattacta
      481 tggcatcata cttattcata tattcccttt cttcaactcc ttcacggcgt gatccgcagc
      541 ccgcgccgta aaggccatat aggtcaatga cggattacag caattggccg agaccatgaa
      601 ggatccgtcc gtcacgaaca cattcttcgc atcccatacc tggttaaaag agttcagcac
      661 cgatgtcttc gggtcgcgac ccatccgcgc ggtacccatc tcgtggatac ccctgcccgg
      721 gaaaggatta tcgcttctgc tcttgatatt cttcactcct gcgatcgtca gcatctctat
      781 cgcatcggcc tccatgtcct tgcgcatctt cagctcgttg tccttcaatt cacaatcgat
      841 agacagcaca ttcagccccc atttatcttt tttcgtcttg tccagcgtta ccttgttctc
      901 atgataggga aggatctcgc cgaaaccgcc gatattcatc ctccagggac ccggttccgt
      961 cagcgcttcc ttgtactcct tcccgatgct gtactcgggt acttcatgat catacccctc
     1021 ccggccgccg ccgccctggt acccgaatcc gcgtacatat tcccgcttgt ccccatccag
     1081 gtttctaaac cgggggatgt agatcccatt cgccctgcga ccaaaataat atttatcctc
     1141 ataaccctcc acgataccgc tggcgcctac acccagatga tggtccatga ggttatgtcc
     1201 gagttcgccg ctgctgctac ccaacccgtc aggccagata tcggtagcag agttcatcag
     1261 cacccaggcg ctgttcagcg tactggcatt gaggaagacg atctttgcat gatactcata
     1321 agttttgttg gtctcggcat ccaacacttc gacacccgtt gccttcttct tatccttgtc
     1381 gtataatatc ctcgtaacta tcgaccaggg acgaagcgta aggttctttg tcgccatcgc
     1441 cgccggcagc gtggaggact gcgtcgagaa ataaccgccg aaaggacacc ccagccagca
     1501 tttattccgg aactgacacc cgggccttcc ctcgtgcggc accgtgatat tggccacccg
     1561 cccgatgatc atatgacgat gtcccttgta atgatccttg atccgcgcgg caacgtcctt
     1621 ctccacacag gtcatatcat aaggcggaag gaacgaactg tccggcaaat gccagatacc
     1681 ctccttcgag ccgctgatgc cggcccattt ctctacatac gtataccagt cctcgatatc
     1741 cttatatcgc accggccagt cgacagcgat cccctgtttg ccattttcct cgaaatgaaa
     1801 atcgctccag cgatagctct gccgtcccca catcaggctg cggccgccca catgataacc
     1861 cctgaaccag tcgaaccgct tgacctcggt ataaggacac tcgctgtcct tcacccaata
     1921 atccaggttc cactcgttga gcggataatc gcgcttcaac acagggtagt cttttttcat
     1981 ctcctgggtg gcatgcccgc gatgcgggaa atcccaggcc tctttattgg cgctcggata
     2041 atctttgata tgctcgatat tacgccccct ctccagcata agcactttca aacccttttc
     2101 cgtaagttcc ttggcagccc agcctccgct gatgcccgat ccgatgacga ttgcatcgta
     2161 agtattgtcg gccataatcg ttaccttatt ttatattttt agttctaaat gtcacaaatc
     2221 accacccctt cttcgaggga ctttaactta tccgcctttg ccggaatgaa ttcctgcgcg
     2281 acatacccct tgtatcccgt cgcaacgatc ccccgcatga ttgccggata aaacaattcc
     2341 tgcgtctcat cgatctcatg cctcccgggg actccaccgg tatgataatg cccgaaatat
     2401 tcatgattgt ccgtgatcgt ccttatcacg tcaccttcgt ctatctgcat atgatagata
     2461 tcatacagca gtttgaaatt cggcgatccg atacgcttga ccaattcgac gccccatttg
     2521 gaattgtcgc acatctggtc cttgtggttg atcttgctgt tcagcagctc catatggacg
     2581 atcaccccct tttgctcggc ctgcgacaac acctgcttca gccccgccac gcaattcttc
     2641 aaccccgtct cgtcatccat tccctcgcgg ttcccgctga aacagatcag gttcttatat
     2701 ccggccttcg ataccaggtc gatatgttcc gtataattct tgatcagcgt cggatgatat
     2761 tccgtatggt tccagccctt ggtcagcgag atctccgccc cattgcacat ggacgaaaag
     2821 ataccatgtg cctgcaatac cggccagtct gccggaccta ccaggtcgat agcagaaaaa
     2881 ccaatccgct tcacggccac gcaaagttga tccaacggga tatcaccgta gcaccatcgg
     2941 catacggaat gattgatcgt tccttttaat ttcaatttct ttggtttttt attcgctgcc
     3001 agagcatttg cggaagcact cagtgaagat agcattccgc ctgttcctag tgccgcggta
     3061 cccgcaacaa tggttttgat agcggtacgt cgtgaggatt tattagacat tttattgagc
     3121 acgttgaaag aagaaagtta caaaaaaatc cagttcccgg ccggcggcga cacaattcta
     3181 atgcgccttt aatccatctc taatctcatc cggctgcccc ggtgtgcaac tgttgcttat
     3241 gcgctgcata cgttttgcgc cgaacgatat agatcagcga aaagacgacg attagtatga
     3301 cgggtaggat cacgaccttc atcagcgtct gtgacccggc gagatgatcc gcatccaccg
     3361 cagtggcacc gccagcaata gctgcagact tgaagtgatc gaaccaacca cccataatcg
     3421 gaatgaacag cgaagtagag aacatccccg cgccacccat caacgagatt cccagcgccc
     3481 cggtcttcgg cagatactcg gacacgaacc caagcatcgt gggccag
//
LOCUS       DASYSV010000449         5201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2015110, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000449 DASYSV010000000
VERSION     DASYSV010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015110"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VGQ51_13780"
     CDS             <1..1443
                     /locus_tag="VGQ51_13780"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9037698.1"
                     /translation="IRGPIVDPFHLYCMTHLISFKARICLFALLLLFTGAGAQTLTQR
                     HVAISADNPGFYEWLPEGYQATGKQHWPLLIFLNGSGDFGNGDSSELVYVLKNGPGRL
                     MKEGLWPDSFTVKAKTFRFIVILPEFDSVPKLNELDSVVNYSLAHYNVDTNRVYMTGL
                     SSGGNAVWYYASASVQRARRLAAIIPISAGTLWTGEAGAQAMASAHLAIFAAANQYDS
                     TIPATATIQAIGLINSIVPSIVPRALDTIYDATGHDAWTETYDPAMDLHAGLNAYQWM
                     LQYSRNPADSTAPTVPPPVAQLASLFVQYLAWQQEVALSWTTTAGQPNNYFLVQRSTD
                     GREFKTIDTVRTGSDSANGQYYNAVDAHPVQGDDYYRIAQVSASGQITYSATREVTVP
                     KPPTTVRSFSISPNPAAGMLNVYLEDSVSGAVRIRIIDPEGRTLRTWVFQKLSQSWHQ
                     SIEVGNLEKGDYFIQVSDKEMISTRPFVKL"
     gene            1486..2871
                     /locus_tag="VGQ51_13785"
     CDS             1486..2871
                     /locus_tag="VGQ51_13785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9037699.1"
                     /translation="MKKLYGFLVRGMLFLPGCVILGGTVNAQVQTPRNIPTGPNSSGF
                     YEYLPAGYSSSSTLYPLIVFLHGSGEVGNGGSDLSKVLHHGPPQLIDQGKFPPSFTVN
                     GQTFSFIVISPQFIASPTDSDVSYVIEYAISHYRVDTGRIYLTGLSMGGGATWGYADE
                     TGHAGLLAAAVPVAAGIFWYGVGGAKIIAAANLPIFAAANLNDPVEPSSGTVNAINEI
                     NAAVPHIDPPALDTIYNASGHGGWNNTYNPDSPMHNGLNIYQWMLQYTRDRQIAGGPV
                     PLPVRLTSFAAEPAPGGLRVDLAWSTAFEENNRCFVVERSGDGSVFSAMDTVAAAPDA
                     LNGHAYSTVDPEPTAGHNFYRLAQVDWDGKVTYSGVDEVTIAQGQGALHISPNPAVGT
                     LYLGIRNEFSGDCAVRVIDLSGTTRLKWVFQKTGASWSQGIDIGRLSAGSYFVEVSGE
                     GFREIEAFIKR"
     gene            complement(2878..3153)
                     /locus_tag="VGQ51_13790"
     CDS             complement(2878..3153)
                     /locus_tag="VGQ51_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037700.1"
                     /translation="MILLFALTVTACIINGIFTIVSFFLYVRARLADRSEQFPPDAEE
                     HARRLAGLDRKERFYQSLHFITLFLFVLFGVLILIHLSNHLNNRVQV"
     gene            complement(3150..3488)
                     /locus_tag="VGQ51_13795"
     CDS             complement(3150..3488)
                     /locus_tag="VGQ51_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HEV9037701.1"
                     /translation="MSKKRPNTSGIVYSTDPDFRIEEETPNMQTLPPAQQKLRARLDT
                     KQRAGKAVTLIEGFIGKAEDLESLGRQLKTHCGTGGSAKDGEIIIQGDQRDKITQWLK
                     DHGYSSTKRI"
     gene            complement(3492..4346)
                     /gene="nadC"
                     /locus_tag="VGQ51_13800"
     CDS             complement(3492..4346)
                     /gene="nadC"
                     /locus_tag="VGQ51_13800"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV9037702.1"
                     /translation="MSNFDDQLQALIRNALQEDIGDGDHTTLSCIPPDARGKAVLYIK
                     QDGILAGMEIAEKILKYKEPAAVFTAYKHDGDRMRDGEEAFIAEGSIHTILQCERLIL
                     NCMQRMSGIATLTHQYVDRLRKYKTRVLDTRKTTPNFRLLEKEAVRIGGGINHRFGLY
                     DMILLKDNHVDYAGSIEIAIEKANRYRQTRKPNLKIEVETRSLEEVRRVLAIGMVDRI
                     LLDNFTPRGLLEAMSLIGGRFETEASGGINLNNIEDYARTGVDYISVGGLIHQAQSLD
                     LSLKAVIV"
     gene            complement(4436..4825)
                     /locus_tag="VGQ51_13805"
     CDS             complement(4436..4825)
                     /locus_tag="VGQ51_13805"
                     /inference="COORDINATES: protein motif:HMM:NF027349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HEV9037703.1"
                     /translation="MKRILGLAVLLLSLVLVSFRPLYTIDDITYAIRAGNVGELSRYL
                     DSRVDISLPDKSDTYSKIQAEIIIRDFFSTNGVQNFTIKHKGNNGGGSEFCVGVLQTR
                     NGDYRTTLFVKQKGDKQFLQEIRFQQQ"
     gene            4989..>5201
                     /locus_tag="VGQ51_13810"
     CDS             4989..>5201
                     /locus_tag="VGQ51_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611389.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEV9037704.1"
                     /translation="MAKNKKVILVIMDGWGLGKDTASDAIRHARVPFVSSLYEQYPHT
                     TLVTYGEAVGLPEGQMGNSEVGHLNLG"
BASE COUNT         1140 a         1336 c         1521 g         1204 t
ORIGIN      
        1 ataagaggcc ctatcgtcga tccatttcat ctctattgta tgacacatct gatctctttc
       61 aaggcgcgga tctgcctttt tgccctgctc ctgctcttca cgggggcggg ggcgcagact
      121 cttacgcaac ggcatgtagc catcagtgcc gacaatcccg gtttttacga atggctcccg
      181 gaaggctacc aggctaccgg caaacaacat tggccgctac tgatcttttt aaacggatcg
      241 ggagatttcg gcaacggaga ttcgtcggaa ctggtatatg tgctgaagaa cggaccggga
      301 cgtttaatga aagaaggact gtggcccgat agcttcaccg tcaaagccaa gaccttccgg
      361 ttcatcgtca tcctgcccga attcgattcg gtacccaaac tgaacgaact ggattcagta
      421 gtcaactatt ccctcgctca ttacaacgtc gacaccaatc gggtctacat gacgggattg
      481 agctccgggg gcaatgcggt atggtattat gcttctgcga gtgtgcagcg ggcccgccgg
      541 ctggccgcga tcattccgat atccgcagga acactatgga ccggcgaggc cggggcgcag
      601 gccatggcat cggctcacct cgccatcttc gcggcagcta accagtatga ctccacgata
      661 cccgccacgg ccactatcca ggccatcggt ctcatcaaca gcattgtccc gtccatcgtt
      721 cccagggccc tcgatacaat ctatgacgcc accggtcacg atgcatggac ggagacctat
      781 gacccggcga tggatcttca tgccggcttg aacgcctatc aatggatgct gcaatacagc
      841 cggaatcccg cggattccac tgcacccaca gtccctcccc cggtggcgca gctggcttca
      901 ttattcgtgc aatatctcgc ctggcagcag gaggtcgcct tatcgtggac gacgacagcc
      961 gggcaaccca acaattattt cctcgtccag cgatcgacgg acggaaggga attcaaaacc
     1021 atcgatacgg tcaggactgg cagcgattct gccaacggtc aatactacaa cgcagtggat
     1081 gcacatcctg tgcagggtga tgattactat cggatcgcgc aggtgagcgc ctctggccag
     1141 ataacgtact cggctacacg ggaggtcacg gtacccaagc cgcctaccac cgtcaggagt
     1201 ttttccataa gtcccaatcc ggcggcggga atgttgaatg tgtatctgga agattccgta
     1261 tccggcgcgg tgcggataag gatcatcgac ccggaaggac gcacgctcag gacctgggtc
     1321 tttcaaaagt taagtcagtc gtggcatcaa tccatagagg tcgggaacct ggagaaggga
     1381 gactatttca tccaggtatc ggacaaggag atgatttcca ccaggccttt cgtgaaacta
     1441 tagaataaaa actcaatgac cgcttcatcc atttaaacaa tttctatgaa aaaactctac
     1501 ggctttcttg tccgggggat gctatttctc ccgggctgtg ttattttagg gggtaccgtc
     1561 aatgcccagg tgcaaactcc gagaaacatc cctaccggac ccaacagcag cgggttctat
     1621 gaatacctgc ctgcagggta ttcgtcgagc tcgaccttat atcccctaat agtcttcctg
     1681 catggcagcg gcgaagtggg caacggcggg tccgatctct ccaaggtatt gcaccatggg
     1741 ccgcctcagt tgatcgacca ggggaaattc cctccgagct ttactgtcaa tggccagacc
     1801 ttttctttca tcgtcatctc gcctcaattc atagcgtcgc ccacggacag cgacgtgagc
     1861 tatgtgatcg aatatgcgat ttcgcattat cgcgtggata ccgggcgcat ctatctgacg
     1921 ggactgagta tgggtggcgg cgccacctgg ggttatgcgg atgaaacagg acatgccggt
     1981 ctgctggcag ccgcagtgcc cgttgccgca ggaatattct ggtatggagt cgggggtgcg
     2041 aagatcattg ccgccgccaa tctgccgatc tttgcggccg ccaatctcaa cgacccggtg
     2101 gagccgtctt cggggaccgt caatgccatc aacgagatca atgccgcggt gccgcatatc
     2161 gatccaccgg cgctggatac catctacaat gcgtcgggac acgggggttg gaacaacacc
     2221 tacaatccag acagtcccat gcacaatggt ctgaacatct atcaatggat gttgcagtat
     2281 accagggatc gtcagatcgc cggcggaccg gtcccattgc cggtgcggct gacctctttt
     2341 gcggcggagc cggcgccggg cggattacgg gtggacctgg catggagtac tgccttcgag
     2401 gagaataacc ggtgtttcgt cgtcgagcga tcgggggatg gatcggtctt ttccgcgatg
     2461 gatacggttg cggccgcgcc ggatgcgctg aacggtcatg catacagcac cgtcgacccg
     2521 gagccgacgg cgggtcataa cttttaccgt ctcgcgcagg tggattggga cgggaaggtc
     2581 acttattcag gagtcgacga ggtaacgatc gcgcaggggc agggcgcgct gcatatcagt
     2641 ccgaaccccg cggtcgggac gctatatctc gggatccgaa atgaattttc aggagattgt
     2701 gccgtgcgtg tcatcgatct gtcgggaacg acgcgtttga aatgggtctt ccaaaagaca
     2761 ggggcatcgt ggagtcaggg gatcgatatc ggccggcttt cagcgggaag ctattttgtc
     2821 gaggtgtcgg gtgagggttt ccgcgagata gaggcgttca tcaagcggtg aaacgcctca
     2881 gacctggacc cggttgttca ggtgattgga cagatgaatg aggatcaata ctccgaaaag
     2941 gacgaacagg aacagcgtaa taaaatgtaa ggactggtag aaccgttctt tccggtcgag
     3001 gcctgccagt ctgcgggcat gttcctcagc gtccggcggg aattgctcgc ttcggtcggc
     3061 aagacgagcc ctgacataaa ggaagaatga cacgatagta aagatcccgt ttatgataca
     3121 ggcggtgacg gttagggcga acagcaagat catatccgtt tggtggagga ataaccatgg
     3181 tctttcagcc attgggtgat cttatcgcgc tgatcaccct ggataatgat ctctccgtcc
     3241 tttgcggagc cgccggttcc gcagtgtgtc ttcagctggc gtcccaggga ttcgagatct
     3301 tcggccttgc cgatgaagcc ttcgatgaga gtgacggcct tgccggcgcg ttgtttcgta
     3361 tcgaggcggg ccctgagctt ttgctgcgca ggggggagcg tttgcatgtt cggggtttcc
     3421 tcttctatcc ggaaatcagg gtcggtagag tagacgatgc cgctggtatt gggtcttttc
     3481 ttgctcatga tttatacaat tacagccttg aggctgaggt cgaggctttg agcctgatgg
     3541 ataagaccgc cgacgctgat atagtctaca ccggtgcggg catagtcttc gatattgttc
     3601 aggttaatgc cgccgctggc ttccgtttcg aatcggccgc cgatgagcga catggcttcg
     3661 agcagcccgc ggggagtaaa attgtcgagc aggatcctgt cgaccattcc gatggccagc
     3721 actctcctca cctcttcgag cgagcgggtc tccacttcga tcttcagatt gggtttgcgt
     3781 gtttggcgat agcgattggc cttttcgatg gcgatctcga tgctgccggc gtagtcgaca
     3841 tggttgtcct tgagcaggat catatcgtaa agaccgaagc gatgattgat gccgccgccg
     3901 atgcggacgg cttctttctc gagcaggcgg aaattgggag tggttttgcg ggtatcgagc
     3961 acccttgtct tgtatttccg gagccggtcg acatattggt gcgtcagggt ggcgatgccg
     4021 ctcatccgct gcatgcagtt gaggataaga cgttcgcatt gaaggatagt atggatggaa
     4081 ccttcggcga tgaaggcctc ttcaccgtcg cgcattctat cgccatcatg tttgtaggcg
     4141 gtgaagacgg cagcgggctc tttgtatttg aggatctttt cggcgatctc catgccggca
     4201 aggatgccat cctgtttgat atagaggaca gctttgcccc tggcgtcggg tgggatgcag
     4261 gaaagggtgg tgtggtctcc gtcgccgatg tcttcctgca gggcattgcg aataagggct
     4321 tgtagctggt cgtcaaaatt cgacatgccg caaaattacg aaaaaggtgt ctttgaaagg
     4381 ttcgaatttg gggggatgga agcgggggct gtccgccagg agtgccggga ttgtgtcatt
     4441 gctgctggaa acggatctcc tgcagaaatt gcttatcgcc tttttgtttt acgaaaagcg
     4501 ttgtccgata gtcgccatta cgtgtctgga gcactccgac gcaaaattcc gatcccccgc
     4561 cgttgttgcc tttgtgttta atggtgaagt tctgaacgcc gttggtagag aagaaatcgc
     4621 ggatgatgat ctcggcttgt atcttgctat aggtgtcgct tttatccgga agcgagatat
     4681 ctaccctgct atcgaggtag cgggaaagtt caccaacgtt gcccgcccgg atggcgtagg
     4741 tgatgtcgtc gatcgtatac agagggcgga aggaaaccag gacgagcgag agcaagagca
     4801 ccgccaaccc aagtatgcgt ttcatgttaa tagaatatta gtcgccctgc ttaatcccaa
     4861 aaatatgcca aactgtcaag aagacattaa aatccccctt agtggggagg gaaagggggg
     4921 gccaaaacgc ccgaatgccc taaatttgcg gcgcaggccg ggtgggccgg cctaaaaaag
     4981 tcaagtttat ggcaaaaaac aagaaagtta tactggtcat catggatgga tgggggctcg
     5041 ggaaggacac ggcatcggac gccatccggc atgcccgggt acctttcgtg agctcattat
     5101 acgagcaata tccgcatacg accctggtaa cctatggaga agcggtaggt ttgcccgaag
     5161 ggcagatggg caattccgag gtaggtcatc tcaatctcgg g
//
LOCUS       DASYSV010000450        10953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_474938, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000450 DASYSV010000000
VERSION     DASYSV010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10953
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474938"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGQ51_13815"
     CDS             complement(<1..414)
                     /locus_tag="VGQ51_13815"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV9037705.1"
                     /translation="MNSFGRLFRVHIFGESHGESVGVCIDGCPAGLALPVEDFLPDLE
                     RRKGGIQKGTTPRQEEDLPIFKSGLFKGMSTGSPITIFFENKNTRSADYEKQRAVPRP
                     GHADFVAHKKFGGFEDYRGGGPFSARLTVGMVAAGV"
     gene            complement(407..1663)
                     /gene="aroA"
                     /locus_tag="VGQ51_13820"
     CDS             complement(407..1663)
                     /gene="aroA"
                     /locus_tag="VGQ51_13820"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV9037706.1"
                     /translation="MEMTIQPSALKGAVLAPASKSSMQRACAAALLRIGETVIRHPGH
                     SNDDKAALGMIRDLGAEVSTLPDGTLLVKSNGVMPNGVSEVNIGESGLGLRLFAPLIA
                     LSDRTVRVRGEGSLVNRPMDLFDEIFPLLGVRLVSNGGKLPLEIQGPMRPRDIEIDGS
                     LSSQFLTGLLFAYAASGVSGVSIRVRDLKSKPYIDLTLDVMRAFGWMVENRNYEEFVF
                     GAGRRDGQREYTVEGDWSGGAFLLVAGALAGPITVQGLDVGSTQADKAILSALRSCGA
                     VLKGEGADLLVGPGDLKAFRFDATDCPDLFPPLVALATYCSGETEIRGVSRLAHKESN
                     RGLTLQEEFRKLGVAIELEGDRMIVHGGGPVKGAVVHSRHDHRIAMACAVAALRASGP
                     VTIEVAEAVGKSYPDFYRDLQSLRHE"
     gene            complement(1666..2697)
                     /gene="aroB"
                     /locus_tag="VGQ51_13825"
     CDS             complement(1666..2697)
                     /gene="aroB"
                     /locus_tag="VGQ51_13825"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV9037707.1"
                     /translation="MKKLQYQFSGKPTTYYLDADFGYLEKLVDKDHAVLITDEHIFKG
                     QQKRFGGWNTIVIHAGEEYKVQATVDSVISQLIEMGADRQTTLVGVGGGVVTDITGYV
                     AAIYMRGLSFGFVPTSVLSMVDAAIGGKNGIDVGVYKNMVGTIRQPSFLLHDYSLLKT
                     LPKEEWVNGFAEIIKHAAIRDAALFRELEKNKLGTYQKDKTALARLIRRNVVIKSDIV
                     RQDEFERGDRRLLNFGHTLGHAIENLYELSHGQAISIGMVAASMISEEILDFRETDRV
                     MAVLKKYGLPTLAEFDPKEVLNVMRRDKKKVKETMNYVLLKKLGQGVVKPIPVNELEK
                     LLQSIITAR"
     gene            complement(2706..3797)
                     /locus_tag="VGQ51_13830"
     CDS             complement(2706..3797)
                     /locus_tag="VGQ51_13830"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV9037708.1"
                     /translation="MEATATKSAKEIVQEAWNKRPLIISGPCSAETEEQTVETVKRLA
                     KTGKVDIVRAGIWKPRTRPGSFEGIGTKGLPWLQQARKLTGLPVAVEVATAKQVEDAL
                     HFDIDVLWIGARTTVNPFSVQEVADALRGVDVPVLVKNPINPDIELWTGAVERVAKAG
                     IGQIGLIHRGFSSYGNTEYRNAPMWHLAIEMKRRSPGLMMINDPSHICGRRDILQDIA
                     QKAIDLDMDGLIIETHIDPDKAWSDAKQQITPERLAEMLDNIKWRHETTTERDFITAL
                     EKLREQINHIDDELIQLLGQRMRIADQIGQYKKDNNITILQTNRWNSILEKAFAKGEK
                     LNLSKEFITKYYDAVHMESIGHQNKIMNS"
     gene            complement(3894..5597)
                     /locus_tag="VGQ51_13835"
     CDS             complement(3894..5597)
                     /locus_tag="VGQ51_13835"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV9037709.1"
                     /translation="MMTFEGLGLDSRLVQATDALGFKQPTPIQEQAIPVLLSGTKDLV
                     GLAQTGTGKTAAFGLPLLQLVDAALRYPQALVVCPTRELCMQIVTEIESFKKFMPGIH
                     VQAVYGGTSIGLQIKDLRRGVQIVVATPGRLIDLIERKAINLEQISYVVLDEADEMLN
                     MGFQDDIEFILQNTPKREATWLFSATMPAEIRRVSKKYMQAPKEITVGKMNTANKAID
                     HQYYVTAAHARYETLKRLIDFNPGIYGIIFTRTKADAQNIAEKLTREGYDIDALHGDL
                     TQQQRDKVMGEFREKTLQLLIATDVAARGIDVQGITHVINYELPDDVEVYTHRSGRTG
                     RAGATGIAVSIVHGRELGKLRQIERIVQVPFHKMEIPSGKDVCRKQFFTFMDKLLQAD
                     ISHGDYETYVPMLAEKFAEVSKEEVLKRVAAMEFDRFLKYYENAEDLNVRKSDGATKT
                     GWRQGSDEGRDREPRGGDRKEYGRGEGKARGREQGGNGDYAKLFVNLGTKDGFYKASF
                     LQFILDMSDLRKEVLGRIDMKEMNSWIEIDRRSAEQMIRAIDGKKFKGRRIRMNDANS
                     R"
     gene            complement(5775..6629)
                     /locus_tag="VGQ51_13840"
     CDS             complement(5775..6629)
                     /locus_tag="VGQ51_13840"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV9037710.1"
                     /translation="MKKFDSITVVGVGLISGSWGLALKDRGLVRNVIGVSRTAASAQK
                     AMELGLIDEVLPLEEAVKKSDLIYVAIPVDVTVPVMRQIMDLVTDRQIVADAGSTKWA
                     LCQSLAGHPMRSRFVATHPMWGTEYSGPEAAVRDAFRGRACVICEKEKSAPDAVEVME
                     DLYKDMGMHIVYMNANDHDMHAAYISHISHITSFALANTVLEKEKEESAIFELAGGGF
                     ESTVRLAKSNPSMWLPIFMQNKEHVLDVLNEHIAQLRKFKSCLEKDNSEYLRELMENA
                     NRIRRIIK"
     gene            complement(6626..7822)
                     /locus_tag="VGQ51_13845"
     CDS             complement(6626..7822)
                     /locus_tag="VGQ51_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV9037711.1"
                     /translation="MKTSKRLDGIGEYYFSRKLREIEELNKAGKNVINLGIGSPDLPP
                     HPDVIRVLQEESANPNVHAYQSYKGSPVLRKAVASWYRKWYGVELDPESEVLPLIGSK
                     EGIMHICMTYLDEGDGALVPNPGYPTYRSAVKLAGGNCVEYSLTEDGGWFPDFEELER
                     LVSSGASHGSTDREPAGRIKLMWVNYPNMPTGKLPDRVLFERLVAFGRKHDILICHDN
                     PYSFILNESPMSLLSVPGAKETALELNSLSKSGNMAGWRVGMLCGSKERIDEVLRFKS
                     NMDSGMFLPVQLAAAKALSLGKEWYDGVNAVYRERRGKVFQLLDLLKCEFSKEQAGMF
                     VWAAIPAGYADGYALSDKVLNQAGVFITPGGIFGSAGNRFIRVSLCAPVERFNDSIDR
                     IKKAGI"
     gene            complement(7819..8658)
                     /locus_tag="VGQ51_13850"
     CDS             complement(7819..8658)
                     /locus_tag="VGQ51_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV9037712.1"
                     /translation="MDKQTRVSIQGYEGSFHQMAARQFFGNDVAVITCATFREVVRVA
                     SSRKESDGGVMAIENSIAGSILPNYNLLQKSDLRVVGEIYLQINQHLLVNPGVKLEDI
                     REVHSHHMALLQCIDFLEKHPAWKLVETEDTALSAKHLQQHRSRHHAAIASDLAAELF
                     GLEMLAPNIHTMKNNYTRFLILQREDDAKAVGEPDKASINFHTDHSRGSLARVLTKIA
                     DGGINLSKLQSFPIPGTEWEYSFHADMEFEDMGQFERVIEAIRPLTAAVKVYGVYKKG
                     ITV"
     gene            complement(8887..9450)
                     /locus_tag="VGQ51_13855"
     CDS             complement(8887..9450)
                     /locus_tag="VGQ51_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037713.1"
                     /translation="MHEIEPFYNWRHLYVSEEDERSPFYGRVYSEFEFSQTIYNYYIH
                     PQWDDFGSRTLYLKVLLADYDEHYVVIELFGEWNDAIENDIMNLKREVIDKFFEFGIT
                     KFILIAENVLNFHSGDKDYYEEWFEEVTDAEGWIVCLNMPEQTQYDFKKAKLNRWVEL
                     LDLDNWRTYKPIHLFRLIDGQMAKRLE"
     gene            complement(9489..10304)
                     /gene="murQ"
                     /locus_tag="VGQ51_13860"
     CDS             complement(9489..10304)
                     /gene="murQ"
                     /locus_tag="VGQ51_13860"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEV9037714.1"
                     /translation="MSFEKVTEEESRYHDLDKMSIKDILAGINQEDKTVPDAVSKAIP
                     QIEKLVAAITDKMLAGGRLFYIGAGTSGRLGVLDASEIPPTYGMPFDLIIGVIAGGET
                     AIRKPVENAEDDPEQGWLDLEGYGVADNDIVVGVAASGTTPYVIGALQECRKRGIPTG
                     CIVCNPGSPVAQQSDYPIEVVVGPEFVTGSTRMKSGTAQKLVLNMLSTAVMIRIGRVE
                     DNKMVNMQLTNSKLVDRGTKMVMEGTGLTDYEQAKELLLRSGSVKKAVDSVST"
     gene            complement(10318..>10953)
                     /locus_tag="VGQ51_13865"
     CDS             complement(10318..>10953)
                     /locus_tag="VGQ51_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HEV9037715.1"
                     /translation="CLNPDNRKIVAKALRGAFRGAGVHVTHDLMGAAVAACGTTRGMV
                     CNLGTGSFSCYFDGKKIAKSSPGLGYVLGDEGSGAYLGKKVLQYYLYDTFDEELRYKF
                     DAKYVTNRSEILENVYKKALPNRFLASFTLFLVENRGHYMIENIIEDGLNDFFFQHLC
                     KYRESWKLPIHFVGGVAYGFRDVVRDLCGTYEFELGRILQKPMDGLIKYHQ"
BASE COUNT         2347 a         3668 c         2290 g         2648 t
ORIGIN      
        1 cacaccagcc gccaccattc ccaccgtcag cctcgcagaa aaaggtcctc cgccgcgata
       61 gtcctcaaaa cctccaaatt tcttatgcgc cacaaaatcc gcatgccccg gtcgcggcac
      121 cgcacgctgc ttctcataat ccgcggaccg cgtattctta ttctcgaaaa atatggtgat
      181 cggtgatccc gtactcatcc ccttgaacaa tccgctcttg aagatcggca gatcctcctc
      241 ctgccgtggc gtcgtcccct tctgtatccc gcccttgcgt cgctccagat ccggcaaaaa
      301 atcttccacc ggtaacgcca atcccgccgg acatccgtcg atacagacgc cgacgctttc
      361 cccatgcgat tcgccgaaaa tatgcacccg gaaaagacgg ccaaaactat tcatgacgta
      421 atgattgtag atcgcgataa aaatccgggt aggatttgcc tacggcctcc gccacctcta
      481 tcgtcaccgg acccgacgcc cgcagcgccg ccacggcaca agccatggcg atccggtgat
      541 catgacgcga atgcacaacc gctcctttta cgggacctcc tccatggacg atcatcctgt
      601 cgccctctaa ttcaatggcc acgcccaact tccgaaactc ttcctgcaac gtcagacccc
      661 ggttgctttc cttatgcgcc agccggctca cgcccctgat ctccgtctcc ccggaacaat
      721 aggtcgccag cgccaccagt ggcggaaaaa ggtcaggaca atcggtagcg tcgaaccgaa
      781 atgctttcaa atcccccggc ccaaccagca gatccgctcc ttcccctttc aacaccgccc
      841 cacaactgcg cagagcagat aatatcgcct tgtcggcctg cgtagaccct acatccagcc
      901 cctgcacggt gatcggtccc gccagcgcac ccgctaccag caaaaaggcc ccgccgctcc
      961 aatcgccctc taccgtatat tcccgctgtc cgtcccgacg cccggcgccg aaaacgaatt
     1021 cctcataatt tctattctcc accatccacc cgaacgctcg catcacgtcc aaggtcaggt
     1081 cgatataagg cttgctcttc agatcacgca ccctgatcga aacgcccgac acgcccgacg
     1141 ccgcatacgc gaacagcagt cccgtcagga actgcgagct caacgagccg tcgatctcga
     1201 tatcacgtgg tcgcatcgga ccctgtatct ccaatggcaa tttaccgcca tttgaaacca
     1261 gccgcacccc caataatggg aatatctcat cgaacagatc catcggacga ttaaccaggc
     1321 taccctcgcc acgcacccgg acggtccggt cactcagggc gataagtggc gcgaaaagcc
     1381 tcaaacccaa tccgctctcc ccgatattga cttcggacac cccattcggc atcacgccat
     1441 tactcttcac cagcaacgtc ccatccggca acgtcgatac ctccgctccc aggtccctga
     1501 tcattcccaa tgcagcctta tcatcattgg aatggcccgg gtgacggatc accgtctccc
     1561 ctatccgcaa taacgcggcc gcacaggccc gctgcatgct gctcttcgac gccggcgcaa
     1621 gtaccgcgcc cttcaacgcc gatggctgaa tagtcatctc catactcagc gtgccgttat
     1681 gattgattgt aataatttct ccagctcgtt caccggtatc ggtttgacca ccccctgccc
     1741 cagcttcttc aacaacacat agttcatcgt ctccttcact ttcttcttat ccctgcgcat
     1801 gacgttcagc acttccttcg gatcgaattc cgccagcgtg ggcagcccgt atttcttcaa
     1861 caccgccatc actctgtccg tctccctgaa atccaatatc tcttcagaga tcatactggc
     1921 ggcaaccatc ccgatggaga tcgcctgtcc gtgcgacagc tcatacaggt tctcgatggc
     1981 gtggcccagc gtatgcccaa aattcagcag cctcctgtcc cctcgctcga actcatcctg
     2041 cctgacgata tccgatttga tgacgacatt ccggcgaatg agacgcgcca gcgctgtctt
     2101 atctttctga taggtcccga gcttattctt ttccagttcc ctgaacaacg cagcatcccg
     2161 tatcgcagca tgtttgatga tctccgcaaa cccgttcacc cattcctctt tcggcagggt
     2221 cttcagcagg ctataatcat ggagcagaaa ggaaggctgc cggatcgtcc ctaccatatt
     2281 tttatagacc ccgacatcga taccattctt tccaccgatg gccgcatcta ccatcgacag
     2341 caccgaggtc gggacaaatc cgaacgacaa cccgcgcata tagatcgcgg cgacataacc
     2401 cgtgatatcg gtgactaccc ctccgccgac gccgaccagc gtggtctgcc ggtccgcacc
     2461 catctcgatc agttgactga taacggaatc cacggtcgcc tgcactttgt actcctcgcc
     2521 ggcatggatg acaatggtat tccagccgcc aaatcgtttc tgctggcctt taaagatatg
     2581 ctcatcggtg atcagcaccg catggtcctt gtcgaccagc ttctccaggt agccgaagtc
     2641 ggcatccagg taataagtcg tcggcttgcc cgaaaattgg tactgtagtt tcttcatata
     2701 aaggatcagg aattcatgat cttgttctga tggccgatcg attccatatg tacggcatca
     2761 tagtatttag tgatgaattc cttgctcaaa ttcagttttt cccccttcgc aaaagccttc
     2821 tccaggatcg aattccaccg gttcgtctgc aggatcgtaa tattgttgtc ctttttgtat
     2881 tgcccgatct ggtcggcgat ccgcatccgc tgacccagca gctggatcag ctcgtcatcg
     2941 atatgattga tctgttcgcg cagtttttca agcgccgtaa taaagtcacg ctcagtagtc
     3001 gtctcatgtc tccatttgat gttgtcgagc atttcggcca gccgttccgg cgtgatctgc
     3061 tgcttggcgt cgctccacgc cttgtcgggg tcgatatgcg tctcgatgat caatccgtcc
     3121 atgtcgagat cgatcgcctt ctgcgcaata tcctgcagga tatcccggcg accgcagata
     3181 tgcgaaggat cgttgatcat catcagtccc ggcgaccgcc ttttcatctc gatagccaga
     3241 tgccacatcg gcgcattccg gtattcggta tttccataag aactaaatcc ccgatggatc
     3301 agccctatct gcccgatacc ggccttcgcc accctttcga cggcgcccgt ccacaattca
     3361 atatccggat tgatagggtt tttgacgagc acgggcacat ccacgccacg aagcgcatcg
     3421 gctacctcct gcacgctgaa aggattcacg gtcgtccgcg ccccgatcca taacacgtct
     3481 atatcgaaat gcagcgcatc ttccacctgt ttggcggtag caacctcaac ggcgaccggc
     3541 agtcccgtca gcttcctcgc ctgttgcagc cacggcaaac ccttcgtccc gatgccctca
     3601 aaactacccg gacgggtacg cggtttccag ataccggccc gcacgatatc gaccttgccg
     3661 gtcttggcaa gtcgtttcac ggtctccacc gtttgctctt ccgtctccgc gctgcacgga
     3721 ccggaaatga tcaggggacg cttgttccag gcttcctgga cgatctcctt tgcactctta
     3781 gttgctgttg cttccatatg aggtgtagtt taacttctgt tttgctgccg ccgcggctca
     3841 cttccattgc tcttcccgcc gcggctcact tccatcgctc ttcccgccgc ggctcaccgg
     3901 ctattcgcat cattcatccg gatccgccgg cctttaaatt tttttccatc gatcgcacgt
     3961 atcatctgct ccgccgagcg ccggtcgatc tcgatccagc tgttcatctc cttcatgtcg
     4021 atccttccca gcacttcctt ccgcagatcg ctcatatcca ggatgaactg aaggaagctt
     4081 gccttataga acccatcctt cgtccccaga ttgacgaaca gcttggcata atcgccattg
     4141 ccgccctgct cccttccccg cgctttcccc tcgccgcgtc catactcctt ccgatccccg
     4201 ccacgcggtt cccggtcccg gccctcgtcg gacccctggc gccaccctgt cttcgtggcg
     4261 ccgtccgact tccgcacatt caggtcttcc gcattctcat aatatttcag gaaccggtcg
     4321 aattccatcg ccgccacccg tttcaacacc tcttccttgc tcacctccgc aaatttctcc
     4381 gccagcatcg ggacatacgt ctcataatct ccgtgactga tatcggcctg cagcagctta
     4441 tccatgaagg taaagaactg tttcctgcac acatccttcc cactgggtat ctccatctta
     4501 tggaacggca cctggacgat ccgctcgatc tgccgtaatt tccccaattc cctgccgtgg
     4561 acgatcgata ccgcgatccc cgtggctccc gctcttccgg tccggccact cctatgcgta
     4621 tatacttcta cgtcatcggg taactcgtaa ttgatgacat gcgtaattcc ctgtacatcg
     4681 atacctctcg ccgccacatc ggtggcgatc agcaactgca acgtcttctc cctgaactcc
     4741 cccatcacct tgtcgcgctg ctgctgcgtc aggtccccgt gcaatgcgtc gatatcgtat
     4801 ccttcccggg tcagcttttc ggcgatgttc tgcgcgtccg ctttcgtccg cgtaaagatg
     4861 atgccgtaga tgccggggtt gaaatcgatc agccgtttca atgtctcata acgggcatga
     4921 gccgccgtga catagtactg atgatcgata gccttgttgg ccgtattcat cttcccgacc
     4981 gtgatctcct tgggggcctg catgtatttc ttgctgaccc tccttatctc cgcgggcatc
     5041 gtggcgctga acaaccaggt cgcttcccgc ttgggcgtat tctgcaggat gaactcgatg
     5101 tcgtcctgga accccatatt taacatctca tccgcctcat ccagcaccac atagcttatc
     5161 tgctccaggt tgatcgcttt ccgctcgatc agatcgatca accgccctgg cgttgccact
     5221 actatctgga cacccctgcg caggtccttg atctgcaatc cgatggaagt ccccccatat
     5281 acggcctgca catgtatgcc gggcataaac ttcttgaagg actcgatctc tgtcacgatc
     5341 tgcatacaca attcccgggt cggacatact accagagcct gcggataacg cagcgccgca
     5401 tccaccagct gtagcaacgg aagaccgaaa gccgccgtct tgcccgtacc agtctgcgcc
     5461 agacccacca ggtccttggt accgcttaac aatacgggaa tagcctgctc ctgtatcggt
     5521 gtgggttgct tgaatcccag cgcatccgtt gcctgcacca accggctatc cagacctaac
     5581 ccttcgaatg tcatcatatt ctttcgaaat ttgccgcaaa ggtactgaat ttgggcctcc
     5641 gggtcccctt aaaaataccc tccggcccaa atcccctaat aatgagcctt cggccgtcgg
     5701 gcccgtggcc cgcctcacag cgccattccc ccgaaactta tcccgcttcc cagcagtcgc
     5761 ccccgcgggc agccctattt gattatcctc ctgatcctgt tcgcattttc cattaactcc
     5821 ctcaaatact cggagttatc cttctccaaa caactcttga acttcctcag ctgcgcgata
     5881 tgctcattca acacatccag cacatgctct ttattctgca taaagatcgg caaccacatc
     5941 gacggattgc tcttcgccag ccgcaccgta ctctcgaaac cgcctcccgc caactcgaat
     6001 atcgcgctct cctccttttc cttctccagc accgtattgg ccagcgcaaa cgacgtaata
     6061 tgtgagatat ggctgatata cgcggcatgc atgtcatgat cgttcgcatt catatagacg
     6121 atatgcatgc ccatatcttt atataagtcc tccataactt ccacggcatc gggcgcactt
     6181 ttctccttct cgcagatgac gcatgccctg ccccggaatg catccctcac cgccgcttcc
     6241 ggtccgctgt attcggtccc ccacatcggg tgcgtggcta cgaaccggct ccgcatcgga
     6301 tgaccggcca gactctggca caacgcccac ttcgtggacc ccgcatcggc tacgatctgc
     6361 ctgtccgtca ccagatccat gatctgtcgc attaccggca ccgtgacatc cacagggata
     6421 gccacataga tcagatcgct cttcttcacc gcctcctcca gcggcaggac ctcgtcgatc
     6481 aatcccagct ccatcgcttt ttgcgcgctc gccgccgttc ggctcacacc gatgacattg
     6541 cggaccagac cccggtcctt caaagccagg ccccagctgc cgctgatcaa tcccactccc
     6601 actaccgtta tcgagtcgaa ctttttcata tccccgcttt ttttatccgg tcgatactat
     6661 cgttgaatcg ttccacaggc gcgcaaaggc ttacccttat gaaccggttg ccggccgatc
     6721 cgaagatacc cccaggcgtt ataaagaccc cggcctgatt cagcaccttg tcactcaacg
     6781 catacccgtc ggcatacccc gccggtatcg ccgcccagac gaacatcccc gcctgctcct
     6841 tactgaattc gcatttcaac agatccagca gctggaatac cttacctctt ctctcgcgat
     6901 aaacggcatt cacgccgtca taccactcct tgcccagcga cagcgccttc gccgcggcca
     6961 gctgcaccgg caggaacatc ccgctgtcca tattgctctt aaatcgtaaa acctcatcga
     7021 tacgctcctt cgagccgcac aacatcccca ctctccatcc ggccatattc ccgctcttgc
     7081 tcagtgagtt cagctccagg gccgtttcct tcgcccccgg caccgacaaa aggctcatcg
     7141 gcgactcgtt caggataaag ctgtatgggt tatcatgaca gatcaaaatg tcgtgctttc
     7201 ggccaaatgc caccaaccgc tcgaatagaa cccggtccgg cagcttcccc gtaggcatat
     7261 tcggataatt cacccacatc agtttgatgc gacccgcggg ctcacggtcc gtcgatccat
     7321 gagatgcgcc cgaactcaca agcctttcca actcctcgaa atcggggaac caaccgccat
     7381 cctccgtcag cgaatattcc acacagttac cccccgccag cttcactgcg cttcgatagg
     7441 tcggataccc gggattgggc accaacgccc catccccctc atccaggtag gtcatacaga
     7501 tatgcatgat cccttctttc gacccgatca gcggcaatac ctcgctttca ggatccaact
     7561 ccactccata ccacttgcgg taccacgacg cgaccgcctt ccgcaatacc ggcgatccct
     7621 tgtaggattg ataggcatga acattcggat tcgcgctttc ctcctgcagc acacggatga
     7681 catccggatg cggaggcaga tcgggactgc cgattcccag gttgatcacg ttctttcccg
     7741 ctttattcaa ttcttctatc tcccgcaact tccgcgaaaa ataatattcc cctattccgt
     7801 cgagccgctt ggatgtcttc atacagtaat tcctttttta taaacgccat agactttcac
     7861 agcggccgtc aatggcctga tcgcctcgat cacccgctcg aattgcccca tatcctcgaa
     7921 ttccatatcc gcatgaaaag aatattccca ttctgtaccc ggtatcggga aagattgcag
     7981 cttgctcaga ttgatccctc cgtcggcgat cttcgtcagc acccgggcca ggctgccccg
     8041 cgaatggtcc gtgtgaaaat tgatcgatgc cttatccggc tccccgacgg ccttcgcatc
     8101 gtcttcacgc tgcaggatga gaaatcgggt atagttattc ttcatcgtat ggatattcgg
     8161 cgccagcatc tccagtccga aaagctcggc agccaggtcg ctcgcgatcg ccgcgtgatg
     8221 tctgctgcga tgttgttgga gatgtttggc actcaacgcc gtgtcctcgg tctccaccag
     8281 cttccaggcg ggatgcttct ccaggaaatc gatgcactga agcaaggcca tatggtgcga
     8341 atgcacctcc cggatatctt ccagcttcac ccccgggttc accagcaaat gctggttgat
     8401 ctgcaaatag atctccccca cgacgcgaag gtcgctcttc tgtagcaggt tataattggg
     8461 caaaatgctg cccgctatcg aattctcgat ggccatcacc cctccatcgc tctctttcct
     8521 gctcgatgcc acccggacca cctccctgaa cgtcgcgcac gtgatcaccg ccacatcatt
     8581 accaaaaaat tgccgcgccg ccatctgatg aaagctgcct tcatatccct ggatagagac
     8641 tcttgtttgt ttgtccatac aaaaaaaaga gtcccgggtt ttgaccggga ctctttatgt
     8701 taattttttc tatttattta tttcttaaca aaagcaatcc cggtctacct gtaaagtaga
     8761 agtaaaagaa aaagtaaaaa taacggtatg ccttcgtggt aatcatatca gtgcaccaaa
     8821 attagggact tctcactaat aaccaaattt atttttgccc gcaacccccg cgcggctacg
     8881 cggccatcat tccaatcgct tcgccatctg cccatcgatc agcctgaaca aatgtatcgg
     8941 cttatacgtc ctccaattat ccagatccag cagctctacc caccgattca gcttcgcttt
     9001 cttaaaatca tactgcgtct gctccggcat attcaaacac acgatccacc cctctgcgtc
     9061 cgtcacctcc tcgaaccact cctcataata atccttatca ccactgtgaa agttcagaac
     9121 attctccgca atgaggataa acttcgtgat cccgaattcg aagaatttgt cgatcacctc
     9181 ccgcttcaga ttcatgatgt cattctcgat cgcatcattc cactccccaa acagctcgat
     9241 caccacatag tgctcatcgt aatcggccag caacactttc agatataatg tccttgatcc
     9301 gaaatcatcc cattgaggat ggatatagta gttgtaaatg gtctgtgaaa actcgaactc
     9361 cgaataaacc cttccataaa aaggcgatcg ctcgtcttcc tcgctcacat acaaatgacg
     9421 ccaattgtaa aaaggttcta tttcgtgcat agctgaaatg cattttttgt ccttcgcact
     9481 ctcccacctc aggtactcac cgaatcgacc gcttttttta cactccccga ccggagcagc
     9541 aattccttcg cctgctcata atccgtaagc cccgtccctt ccatcaccat cttagtaccg
     9601 cgatccacca atttgctgtt cgtcagctgc atattcacca tcttattgtc ctctaccctt
     9661 ccgatccgga tcataactgc cgtggacagc atatttaata ccagcttctg ggccgtgcca
     9721 ctcttcatcc tggtgctgcc cgtcacaaat tcagggccaa ccaccacttc tatcggataa
     9781 tcggactgct gcgctaccgg cgaaccggga ttgcagacaa tacacccggt agggataccc
     9841 cgcttccggc attcctgcaa cgcaccgatc acatagggcg tcgtcccgct ggccgccaca
     9901 cctacgacaa tatcattgtc cgccacgccg taaccctcaa gatccaacca gccctgctcc
     9961 gggtcgtcct cagcattctc caccggcttg cggatggccg tctccccgcc cgcgatgacg
    10021 ccgatgatca gatcgaacgg catcccataa gtcggaggta tctcgctggc atccaatacg
    10081 cccagccggc cgctcgtgcc cgcgccaata taaaaaagac gaccccctgc cagcatttta
    10141 tccgtaatgg ccgccaccag cttctcgatc tgcggaatgg ccttgcttac ggcgtcggga
    10201 acggtcttgt cctcctggtt gatacccgct agtatatctt taatactcat cttatccagg
    10261 tcgtgataac ggctttcttc ctcggtaacc ttctcaaatg acatgcgtat aggttcttta
    10321 ttgatgatat ttaattagtc cgtccatcgg cttttgcaat atccggccca gctcgaattc
    10381 ataggtaccg cacagatccc tcacaacgtc ccggaatccg taagccacgc cgcctacaaa
    10441 atggatcggc aatttccagc tttcccggta cttacacaaa tgttggaaga aaaaatcgtt
    10501 cagtccgtcc tcgatgatat tctcgatcat atagtgcccg cggttctcca caaggaacag
    10561 ggtgaatgaa gccaggaacc ggttgggcaa cgctttctta tacacatttt caaggatctc
    10621 gctcctgttg gtaacgtatt tggcatcaaa cttatatcgt agctcctcgt caaaggtatc
    10681 ataaagatag tactgcaaca ccttcttacc caggtaggcc ccgctgcctt cgtcccccag
    10741 tacatacccc agccccggac tgctctttgc gatctttttg ccgtcaaaat aacaggagaa
    10801 actgccggta ccgagattac agaccatccc cctggtggta ccgcaagccg cgacggccgc
    10861 ccccatcagg tcatgggtca catgcacgcc cgccccccgg aatgcccccc gaagggcttt
    10921 tgccacgatc ttccgattgt ccggattcag aca
//
LOCUS       DASYSV010000451         7562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_475118, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000451 DASYSV010000000
VERSION     DASYSV010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475118"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VGQ51_13870"
     CDS             complement(<1..843)
                     /locus_tag="VGQ51_13870"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEV9037716.1"
                     /translation="MSIKRRDFIRLSAGNAALLYGLPVGLAAGAEHGKTSDKTAGSGS
                     SFGMNGERAGALDRLAPMTGDVVPISVEERQARIQKAQKLMGENNIQAMVLDSGTSMV
                     YFTGIGWGASERPMLAIIPAHGEVKYISPAFEAERLRELIKIGNEVRVWEEDESPYKV
                     AAGILKDYGIRTGTVGMEERVRFFIFDGIRKEASQLNYVSADPVTAACRMIKTPAELA
                     LIQKANDITVAAYKACIALLHEGMTPADFSSSAEDAFSRLGVRGGLSANFGEASAFPH
                     GSIQT"
     gene            891..3278
                     /locus_tag="VGQ51_13875"
     CDS             891..3278
                     /locus_tag="VGQ51_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037717.1"
                     /translation="MLKNLIIVALRNFRRDKWYTLLNILGLTIGITFSLFLIFYILDE
                     LSYDRYNQKADRIYRIGSFIKEANKPVMKWPSTQYPLGPALAKEYPEVEESVRFVGKG
                     KTLYKIGDKTFYEEKIFYADSNAFRVFTYPFLEGDPRTALVAPKSIVLTESVAKQFFG
                     NSHPVVGQSLKDGSGDYFKVTGVVKDVPANSHFRFNMLISNSTLDKNFANNWGGFFMF
                     TYVLLRPNTNSAAFEKKLLALYDEHMAEIFAKFNVKIHYVVEPITWIHLHSDLDGEPE
                     ETGSMSYIYIFSAVALFMLIIACINYMNLTTARSARRAKEIGVRKVTGSTKPQLVAQF
                     LIESTAMTVIALLLSMGLVALLLPTFNTLSGKSISFGALLEPDIFGILIAIILFVGLL
                     GGSYPALYLSKFDPIHILKGNLSKGSSNVTLRRVLVVTQFSIAMIMLICTLVVYGQLN
                     YLRNIDIGYDRARVATLAVNGGRNANGQIRTFTNEIRKDPHCIYASTAQAVPGQDIGL
                     NLYSVQTDKGFTDQGVNNYAVDENYFKTMGIKIVKGRDFSGLPDTANSIIVNQNMVKF
                     FGWGDNPIGKRVKFTGDTSQFHFEVVGVVKDFNQRSLYNPIAPLMFFYRPNSNIIQVK
                     LDGKDIAAGVTALQKAWHTAFPDLPFEYKFLDQTFNSAYEADQKRGRLFTTFSVVTIL
                     ITCLGLLGLIAFTTQQRQKEISVRKVMGAGTGQLVILITRNFVALVAFSCIFACPAAW
                     YFMSKWLKIFPYNTGLSFTPFLLASVTVLVITLLTVIFHTMRAALASPALSLRSE"
     gene            complement(3265..4104)
                     /locus_tag="VGQ51_13880"
     CDS             complement(3265..4104)
                     /locus_tag="VGQ51_13880"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEV9037718.1"
                     /translation="MNKSSLYLKGERLKELHSRPGIFVIPNPWDAGSAKILAHIGFEA
                     LATTSAGLAFSLGKPDGMRVVTREEALANARAIVEATPLPVSADLENGYGDEPSVCAE
                     TVRMAADAGLAGCSIEDATGDPADPIYPFELAVQRVSAAVAAARSFPFPFTLTARAEN
                     LIHGRPDLKDTIRRLEAFAAAGADVLFAPGLTTKDEIGAVVRAVAPRPVNVVMGLGRG
                     SFTLDELAELGVRRVSLGSTLSRAAYGPFWRAAEEIRQHGTFDFAKDMVAYSEFNQLF
                     TRS"
     gene            complement(4097..5821)
                     /locus_tag="VGQ51_13885"
     CDS             complement(4097..5821)
                     /locus_tag="VGQ51_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEV9037719.1"
                     /translation="MVRSALISLFLMWHIRAAAQYYFTGEVQGMHGDKLQHVSITVKS
                     SGSVFLTGVDGDFSIVSRQSDDTLTFVYDGYEHFTTSIKSTDFLQVTLKATPATGMAG
                     DRLVSMISGGGPSAYGGAGGLRSDLVENPFVAQPATVSFAGSCGGISYSIIRRFLEMG
                     TPVPADAVKIEEMLDYFDWNEGEPHPGELFHCNSALLSCPWNNAHRLLFLNIHTGKAD
                     MAQLAPANIVLLIDASGSMDLPNKLPLIKEGIRPLVRNLRDIDTISLVVYGSGLNVMA
                     GIPGSEKSRIIEAIEQLRPGGPSPGERGLRLAYEVARHQYITGGNNRIVLMTDGDICN
                     TSTVETEMAEYIAQQSKDGISLSCLGVGLEDTVHSELPAFARLGKGNFSNVREEEEGE
                     KVLLNELTGRGSIADKVSITAEFDTTLIGGYRLIGWDNRRSALQDTAIRLEGCSVCPA
                     HSLTALFEIVPKKDSAQIGAMAQIKISYCLPREHTGRTMSYLCPNELTPFQRAGLPLK
                     RAVCIALFGMKLRHSGYVGQVSWAEVEKMTKESFSTRDFIDRDYLTLVARAKKLYEGK
                     PGEIAIHE"
     gene            complement(5906..>7562)
                     /locus_tag="VGQ51_13890"
     CDS             complement(5906..>7562)
                     /locus_tag="VGQ51_13890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9037720.1"
                     /translation="RLEQEKGVIVRLGLQRFGDRYLHDTFVNGNPEAGRIAYVRLFSG
                     IAVFILLIACINFMNLATARSVARAREIGLRKVVGSMRGPLIAQFFCEALVFAVLSLS
                     LSLIIVVAFLPFFNTFTGKQLSLPFTQPTFWAYAAVLVLVTGLVAGSYPALYLSSLKP
                     IRILKGAVRFSAGAIRFRRSLTVFQFVLSIALIIATLVITRQVSYVQNTRLGYDRENL
                     IYVRIEGDLVNQKNYLLFKQLASTMPGIALVDRCTEALQNMDFIVSDSSIRWEGKRPD
                     EQVGIKPASVGWDFVRLMKMEVVLGRDFSRTMATDSADAFLVNEEAVREMGLKDPIGK
                     WVSAWKKKGHIIGVLKDFHTASLHEPIMPVLLDVKEYEDFGLIVTRTKPGQTNAALAS
                     LESVYKTVNPGYAFSYQFVDEEYKKLYNSELIISRLTVSFAILAILISCLGLLGLAML
                     AARQRIREIGIRKVLGASVRQIVGLFSRDFLGLIAVAFLIAAPLAGYAMQRWLADFAY
                     RIDISWWVFALAGFSAALLALLTVGWQAIQAARVNPATSLRTE"
BASE COUNT         1782 a         2308 c         1827 g         1645 t
ORIGIN      
        1 ggtctgaata ctgccatgtg gaaaggcaga cgcttcaccg aagttggcgc taagccctcc
       61 ccggacgcca agacgcgaga aagcatcttc ggcactggaa gagaaatcgg ctggggtcat
      121 cccttcatgc aacagcgcaa tacaggcttt atacgcggca accgtgatgt cattcgcttt
      181 ttggatcagg gccagctccg ccggagtctt gatcatcctg catgcggccg ttacagggtc
      241 ggcactgaca taattcagct gagacgcctc ttttcttatt ccatcgaaga tgaagaatcg
      301 taccctctct tccataccca cggttccggt gcgaatgcca tagtccttca gaatacccgc
      361 tgccacttta tagggactct catcctcctc ccatacccgc acttcgttac cgatcttgat
      421 caattccctc aatcgctcgg cttcaaaggc aggactgata tatttaactt caccatgtgc
      481 ggggatgatc gccagcatcg gccgctcact cgccccccag ccgatgccgg tgaaatagac
      541 catagaggta cccgagtcca aaaccatcgc ctggatatta ttctctccca tcagcttttg
      601 tgctttctga atacgcgcct ggcgctcttc taccgagatc ggcacgacat cccctgtcat
      661 cggagccaac cggtccaaag caccggcccg ttcgccgttc atcccaaaag aggatccgct
      721 tccggccgtc ttatcagagg tcttgccatg ctccgcccca gccgccagcc ccaccggaag
      781 gccataaagc aatgccgcat tcccggcgga aagccggata aagtctctcc ttttgatact
      841 catgtaaagt tgtttgagcg ggaagataag attttttatc tttaagatat atgctcaaaa
      901 acctgataat cgtcgcactc cgcaatttcc ggagagataa atggtacacg ctcctcaaca
      961 tcctggggct caccatcggc atcacgttca gcctcttcct catcttttat atcctggacg
     1021 agctgagcta cgaccggtac aaccagaagg ccgaccgcat ctaccgtatc ggctccttta
     1081 taaaagaagc caacaagccg gtgatgaagt ggccctccac ccagtatccc ctcggcccgg
     1141 ccctggcaaa ggagtacccg gaagtagagg agtccgtccg gttcgtcggc aaaggaaaaa
     1201 cgctgtataa gatcggcgac aaaacctttt atgaagaaaa gatcttctat gcggatagca
     1261 acgccttccg ggtcttcacc tacccatttc tggaaggtga tccgcgaacg gcccttgtcg
     1321 cacccaaatc catcgttctc acggagtccg tcgccaaaca attcttcggt aacagtcatc
     1381 cggtggtggg acagtccctc aaggacggca gcggggatta tttcaaagtc accggcgtcg
     1441 tcaaagacgt acccgccaat tcgcacttcc ggttcaacat gctgatatcc aacagcaccc
     1501 ttgacaagaa ttttgccaac aactggggcg gcttcttcat gttcacctac gtcctgctgc
     1561 gacccaatac caattcggcc gccttcgaga aaaaactact tgccctgtac gacgaacata
     1621 tggccgaaat tttcgccaag ttcaatgtga aaatacacta tgtagttgag cccatcacct
     1681 ggatccatct gcattccgat ctcgatggcg agccggaaga gacgggcagt atgtcctata
     1741 tctatatttt cagcgccgtg gctctgttta tgctcatcat cgcgtgtatc aattatatga
     1801 acctcacgac ggcacgctcc gccagacgag ccaaagagat cggcgtccgg aaagtcacgg
     1861 gatccaccaa accgcagctc gtcgcgcaat tcctcatcga atccaccgca atgacggtca
     1921 tagccctcct gctcagcatg ggactcgtgg ccctcctgct gccgaccttc aatacccttt
     1981 cagggaaatc catttccttc ggggccttgc tggaaccgga cattttcgga attctcatag
     2041 ccatcatcct tttcgttggc ctcctcggcg gcagctatcc cgccctgtat ctttcgaagt
     2101 tcgatcccat ccatatcctg aaaggcaatc tgtcgaaagg ttccagcaac gtcaccctgc
     2161 gtcgtgtcct ggtcgtcacg caattcagca tcgccatgat catgctcatc tgcacgctcg
     2221 tcgtctacgg acagttaaac taccttcgga acatcgacat cggttacgac cgggcgcgtg
     2281 tggctacact agcggtcaac ggtggccgca atgccaacgg ccagatccgc accttcacca
     2341 atgagatacg taaggatccg cattgtatat acgccagcac cgcccaggca gtccccgggc
     2401 aggacatcgg tctgaacctg tactccgttc aaacggacaa aggcttcaca gaccagggcg
     2461 tcaacaacta tgccgtcgac gagaactatt tcaaaacgat gggaattaag atcgtcaagg
     2521 gcagggattt ctccggcctc ccggataccg ccaatagcat catcgtcaat cagaacatgg
     2581 tgaaattctt cggctgggga gacaatccca tcgggaaaag ggtgaaattc accggcgata
     2641 catcccaatt ccatttcgaa gtagtaggag tagtgaaaga tttcaaccag cggtccctat
     2701 acaacccgat cgctccgctg atgttttttt accgccctaa ttccaatatc atccaggtaa
     2761 agctcgatgg gaaagacatc gccgccggcg tcaccgcctt gcaaaaggcc tggcacaccg
     2821 ccttccccga tctgcctttc gaatacaagt tcctcgacca gacattcaat tccgcatacg
     2881 aggcagacca gaagcgcgga cggctgttta cgaccttttc ggtcgtgacc attctgatca
     2941 cctgcctggg attactcggc ctcattgcct tcactacgca acagcggcaa aaagagatat
     3001 ctgtgcgcaa ggtcatggga gcaggcaccg gccaactggt gatccttatc acacgtaact
     3061 ttgtggcgct ggtggctttc tcctgtatat tcgcctgccc cgccgcatgg tacttcatga
     3121 gcaaatggct gaaaatattc ccttacaata cgggactatc gttcacacca ttcctgctcg
     3181 catcagttac ggtgctcgtc attaccctgc tcaccgtcat tttccatact atgcgtgcgg
     3241 ctctcgccag ccctgcactt tcgcttagga gcgagtgaaa agttgattga attcggaata
     3301 agctaccatg tccttcgcga agtcgaaggt gccgtgttgt cgtatctctt ctgctgcgcg
     3361 ccagaaaggt ccatatgcgg cgcgactaag tgtagaaccc aaacttaccc ggcgaacccc
     3421 gagctccgcc aattcatcca gggtaaaact cccgcgtccc agccccatga ccacattcac
     3481 cggccgcggc gctacagcac gtactacggc gccgatctcg tccttcgtcg tcaaacccgg
     3541 agcgaaaagc acatcggcgc ccgcggccgc aaaggcttcc aggcgacgga tcgtatcctt
     3601 cagatccggc cgtccatgga tgagattttc cgcccttgcc gtcagcgtaa atggaaaggg
     3661 aaaactacgc gccgctgcta cggccgcgct cacccgctga accgccagct cgaaaggata
     3721 gatgggatcg gccggatcgc cggtggcatc ttcgatggaa catcccgcca gtcccgcatc
     3781 cgcagccatt cgaacggtct ccgcacagac cgacggttca tccccatatc cattctccag
     3841 gtccgccgaa acgggcaacg gggtagcttc cacgatcgcg cgggcattgg ccagcgcctc
     3901 ttcgcgcgtc acgacccgca ttccgtcagg cttgcccagc gaaaaggcca atcctgcgct
     3961 ggtggtggcc aacgcttcga atcctatatg cgcaagtatt ttcgccgagc cggcatccca
     4021 ggggttgggg atcacgaata ttcccggacg gctgtgcagc tctttcagtc tttccccctt
     4081 gaggtagagt gaacttttat tcatggatgg ctatttctcc cggcttgcct tcgtaaagct
     4141 tttttgcccg ggccaccaaa gttagataat cccggtcgat aaaatctctg gtcgaaaaag
     4201 actctttggt catcttctct acttccgccc agctgacctg acccacataa cccgagtgac
     4261 gcagcttcat gccgaacagc gcgatacaaa ccgccctttt caatggcaga ccggcccttt
     4321 ggaagggtgt cagctcgttc ggacagagat agctcatcgt tcttccggtg tgctctctcg
     4381 gcaggcaata gctgatcttt atctgcgcca tcgccccgat ctgcgccgaa tcttttttcg
     4441 ggacgatctc gaacaacgcg gtcagcgaat gagccggaca aacgctgcag ccctccagcc
     4501 tgatcgccgt atcctgcaac gcgcttcgcc tgttgtccca cccaatgagc cggtagccgc
     4561 cgatcagcgt agtatcgaat tcggccgtga tcgacacctt atcggcgatg ctgcctctgc
     4621 ccgtcaactc attcaacagg accttctccc cttcctcctc ttcacgtaca ttggagaaat
     4681 tacccttccc cagccgggca aatgcgggaa gttcggaatg gacggtatct tccagcccaa
     4741 cccccaaaca ggaaagcgat atgccatctt tactttgctg cgcgatatac tccgccattt
     4801 ccgtctctac cgtcgaggtg ttgcagatgt cgccgtccgt catcaggact atgcggttgt
     4861 tcccccccgt tatatactga tgcctcgcta cctcataggc cagtctcaac ccacgctcgc
     4921 cgggtgaggg tccccccggc cgaagctgct ctatcgcttc gatgatcctg ctcttctccg
     4981 accccggtat gcccgccatt acgttcaatc cggacccata cacgacgaga ctgatggtat
     5041 cgatatccct tagattacgt accaatggcc ggatgccctc ctttatcagg ggcaatttgt
     5101 tcggcagatc catggagccg gaggcatcga tcagcagcac gatattggca ggagccagct
     5161 gcgccatatc cgcttttccc gtatggatat taaggaaaag caaccggtgc gcgttgttcc
     5221 acggacagga caataacgcg gaattacaat ggaaaagctc ccccggatga ggctcccctt
     5281 cattccaatc gaaatagtcc agcatttctt cgatcttgac tgcgtcggcc gggacgggcg
     5341 tccccatttc caggaaacgc ctgataatac tataggaaat accgccgcaa ctgccggcaa
     5401 aagataccgt cgcaggctga gcgacaaagg gattttccac gaggtcgctc cgtaacccgc
     5461 ccgcaccgcc ataagcagaa ggaccgccgc ccgagatcat cgataccagc ctgtctcctg
     5521 ccatcccggt ggcaggcgtt gccttcagcg tcacctgcag gaaatcggtg gatttgatgg
     5581 aagtggtaaa atgctcgtag ccgtcataga cgaaggtcaa tgtgtcatcg gactgccgcg
     5641 atacgattga aaaatcaccg tctacgcctg tcagaaagac cgagccgcta gacttaaccg
     5701 ttatggatac atgctggagc ttgtcaccgt gcataccctg gacctcgccc gtgaagtaat
     5761 actgggccgc ggcacggata tgccacatca ggaacaacga tatcagggca ctccgtacca
     5821 aatgaagagt tattccccta aagttatgca aaatatgtgc ctttgcaagt tgaggcggct
     5881 acggaacaca aaatccgtgt atcccctact ccgtacgaag actcgtcgca ggattgacac
     5941 gcgcggcctg gatggcctgc catcctaccg tcagcagtgc gagcagggcg gccgaaaaac
     6001 ccgctaatgc aaaaacccac caggagatat ctatcctgta ggcaaaatcg gccaaccacc
     6061 gctgcatggc atacccggcc aacggcgcgg cgatgagaaa agccacggcg atcaggccca
     6121 ggaaatccct ggagaataag ccgacgatct gtcggaccga ggcccccagt accttccgga
     6181 tgccgatctc cctgatacgc tgtcttgcgg ccagcatcgc gagccccaac agacccagac
     6241 aggagattag gatagccagg atagcgaaag atacagtcag ccgggagatg atcagctcgc
     6301 tgttgtacag ctttttatat tcttcatcca cgaactgata ggaaaaggca taacccgggt
     6361 tcacggtctt gtataccgat tccaggctgg ccagcgccgc attcgtctgc ccgggcttcg
     6421 ttcgcgtgac gatcaacccg aaatcctcat attctttgac gtcgaggagc accggcatta
     6481 tcggctcatg gagcgacgcg gtatgaaaat ccttcagcac cccgatgata tgacctttct
     6541 ttttccaggc ggatacccat tttccgatcg ggtctttcaa ccccatctcc ctcaccgcct
     6601 cttcgttaac caaaaaagca tccgcagaat ccgtggccat cgtccgtgaa aaatcccggc
     6661 cgaggaccac ctccatcttc atcagccgga caaagtccca ccccacagag gctggtttga
     6721 tccctacctg ctcgtcggga cgcttgcctt cccatcgaat ggacgagtcg gagacaatga
     6781 aatccatatt ttgaagggct tcggtgcatc ggtcgaccaa agcgatcccg ggcatggtcg
     6841 aagccaactg cttgaacaga aggtaattct tttgattgac caggtcaccc tctatacgaa
     6901 catagatcag gttctccctg tcgtatccca gccgggtatt ttgcacatag ctcacctggc
     6961 gcgttatcac cagtgtcgcg atgatcagcg cgatcgacag cacaaactga aaaaccgtca
     7021 gcgatcttcg gaacctgatc gcgcctgccg aaaaccgaac tgctcctttc aggatacgga
     7081 taggcttcag cgaagagaga tataatgcag gataagatcc cgctaccagg ccggtaacga
     7141 ggaccaggac ggctgcatac gcccagaagg tcggttgggt gaacggaagg gacagttgct
     7201 tcccggtaaa ggtgttgaag aaaggcagga aagcaacgac gatgatcagg gagagggaga
     7261 gcgataagac ggcaaagacg agggcttcac aaaagaactg cgcgatcaaa ggtcccctca
     7321 tagagccgac taccttacgc aacccgatct cacgggcgcg ggcaaccgat ctggccgtcg
     7381 ctaggttcat gaaattgatg caggctatca acaggataaa aacggcgatc ccgctgaaca
     7441 accgcacata agctatcctg cccgcctccg ggtttccgtt cacgaacgta tcgtggagat
     7501 accgatcgcc aaaacgctgg agccccagcc ggacgataac ccccttttcc tgctccagcc
     7561 gg
//
LOCUS       DASYSV010000452         7704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2524981, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000452 DASYSV010000000
VERSION     DASYSV010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524981"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1051
                     /locus_tag="VGQ51_13895"
     CDS             188..1051
                     /locus_tag="VGQ51_13895"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HEV9037721.1"
                     /translation="MTIDAVHMVLYLVVGAILLGLPAYGAAFFRRRRLRQLFADKHRD
                     YDDLLGRYNPYYKSLSIAGRDRFLQRVLDFMEAKKFDYIDVAPQEIMPLLISATAVQL
                     TFGLEHYLLDHFRTIHILQDRYRYGLYNQPFEGHVAEDGIYFSWNHFFREYADYSDGQ
                     NVGLHEMAHALTYVNFTVQEGRDSTFHDHFIEFSAVARPIFQRMQTGETTLLDPYAAT
                     NYQEFWAVCVETFFERSNSMKKQMPELYSALCILLNQDPLTADKLLTVETGAVSLPPS
                     PAPANLGPAGP"
     gene            1278..2129
                     /locus_tag="VGQ51_13900"
     CDS             1278..2129
                     /locus_tag="VGQ51_13900"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HEV9037722.1"
                     /translation="MEIVVIFSLVLGILLLNRPVTVLFQRLYTRHQFKRFIASETFYH
                     AVVSRHFRYYTRLGLDEQRKFLFRTYLFQHSKNFHYIEVEQSAEMPILVSAAAVQLTF
                     GLEKFKLNYFDDIFILRDDYHYGFYSRPFMGHVDQTGIYLSWDNFMKGITGQTTNCNV
                     GLHEMAHALAYVNFITRTEEDKHFKREFPNFSKVARPIFTSMQEGIKNLLGDYAATNY
                     HEFWAVAVEIFFESSVQFRHELPELYKAMSGVLNQDPLGCLTGSTTILVRAESAPVQM
                     AGAEITA"
     gene            complement(2139..3221)
                     /locus_tag="VGQ51_13905"
     CDS             complement(2139..3221)
                     /locus_tag="VGQ51_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9037723.1"
                     /translation="MRQQTDILIIGQGICGTFLSLELERAGISHLILDDKRPLSASRA
                     AAGLINPVTGRRIVTTWMIEELLSFARDAYGRLPGLLGSSFFETATVVDFFPTPQMRL
                     AFLQRLGENASYLRMPTDEHGWDDVFRSDFGFGVISPCHLVDLPGLLDAARRRMLEKG
                     FLREERFEREELILVSGDMVLYKDLEARKIIFCDGIEGASDPWFSRLPFAPNKGEALV
                     VSIEGLAERLAGQKTIFKRGISLAPWRDGTWWVGSSYEWAFGHGEPTDAFRHRTETLL
                     REWLKLPFRTLQHLASVRPATLERRPFVGFHPTHPAVGILNGMGTKGCSLAPYFANQL
                     VRYITAGTPILPEADVRRFSKVLGRH"
     gene            complement(3338..>7704)
                     /locus_tag="VGQ51_13910"
     CDS             complement(3338..>7704)
                     /locus_tag="VGQ51_13910"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEV9037724.1"
                     /translation="VRGNSRGSSDSVRYEKDNLTTWIFLDRSIYRPGQPLYFKGIVTS
                     RDFSTRRVKIRAGFHTRVFLIDANGTKVDSIAVTTNEFGSYHGRFTLPQGLLNGQFML
                     QDDSTDQSKAFYVEEYKRPKFYVSFDTLKTNYRIGDTIRMEGYAKAYAGNGIDGALIK
                     YRVTRQLAWNRWSYNRSADEISHGTLRTDANGRFRISFKAKPDKSIQKGYEPIFEYTI
                     SADVTDISGETHTSQGSVKAGYRGLELTIGQPVEDHISADSCNMIVVNAVNLAGIPQP
                     VLVEISIYGLQSPNRLIRERYWPAPDQFILTKEAFLHDFPHDEYGDESDPKSWQLGQK
                     VFGATDSAGKSISVTGISGKGLAPGWYIIEAIAHDANGQPVKTERYVELYDGSTGKPA
                     RPLYDWTMQADQTTEPGNRALVSIGSSAGSVFVIRAVERMEEVSQNRSFRFLTLDRDR
                     KQVEIPVTEADRGGLGVSDVFIKDNRIYTRLSTINVPWTNKELSIRYLSYRDKTLPGS
                     LENWEVTIEGSRAEKVASEVLAGMYDASLDQFKPHRWVVPHLYEVFEPFWKWNGIDFA
                     SDRSERRNTYGSDIRYFFPVYDRLLSVDNQEIRERLLEGRMSFRTGPAPGMAKFDVSA
                     GYMAMQVPDMEKDADGSKLLMKDSIVVNTGKSPQPSSATAPPPAVQARKDFRETAFFL
                     PDLRTDSAGNVSFSFTMPEAVTSWKWMTLAHTRDLAFGYGEKTIITQKELMVQPNAPR
                     FLREGDRMELPVKVVNLTDSELTGQMNLSLTDPTTGETADGWFTNRQANQYFTVGARQ
                     SVVVSFPLDIPYQYNRPVTYKVVAAAGAYSDGEEATLPVVSNRIMVTESLPLNMPGDG
                     TRHFTFDKLLKSGSSETLNHHALTVEFTANPAWYAVQSLPYLMEYPHECSEQVFERVY
                     ANALASHIAGASPRLKDMFDRWRGTDTAQLLSNLQKNQELKAVLLEETPWVMQGKSEA
                     QQKKNLALLFDMHRMSTELRAAIDRLSKMQSEGGGFPWWSGGPDNRHITQYILTGIGH
                     LRRLRAVSPDLAGRLDTIVAAALPYLDEQINKDYHRVKARPAEDLIGPMQVHYLYMRS
                     FFANSGLPGTAFAAVNYFRKKAQRDWLKCSPYLQGMIALALYRTGDIQTARDIIASLK
                     QTASRDPERGMYWKGMEGGYYWWQAPVETQSLLIEAFHEISGDAAIDRDLKTWLLKQK
                     QTHSWPTTTATADACYALLMGGDNWLNAERSVDMRLGDKTIDWPAGSGDAGVGYNKKV
                     FDGAFVNPTMGNINVSMETKGGGGSPAWGAVYWQYFDMSNRITPPGGAKPALSVMKKL
                     FIRRNTDRGPLLEPLADNGELKPGDRVVARIELRADRDMEYVHMKDMRAACLEPVNVL
                     SQYKWQDGLGYYESTKDASTDFFFDVLPRGTYVFEYDMLAGQSGNFSNGITTIECLYA
                     PEFTWHTEGIRVNVETGN"
BASE COUNT         1616 a         2213 c         1996 g         1879 t
ORIGIN      
        1 cggatggtct gtacccatcc cgccaatgtg acgttctgac cgatctgagc gctccttagt
       61 tccccgcaag tgtgtgttcg atacattttc caggctattt acgaggtgca aaggtattta
      121 aaaccaatca ggtggcaagg ggcggggtcc gtttggctaa tgtgttaaaa acctgtattt
      181 tcagggaatg acgattgacg ctgtccatat ggtcctgtac ctggtggtcg gggctatcct
      241 attgggtctg cccgcgtatg gcgccgcatt tttccggcga cgcaggcttc gccagctgtt
      301 tgcggacaaa caccgcgact atgacgacct gctcggtcgc tataatcctt attataagag
      361 tcttagcatt gccggccggg atcgtttcct gcaaagggtg ctggatttta tggaggcaaa
      421 gaaattcgat tacatcgatg tagccccgca ggagatcatg ccgctgctga taagcgccac
      481 ggcggtgcag ctgaccttcg gtctggaaca ttacctgctc gatcatttcc ggaccatcca
      541 tattctccag gaccgttacc ggtatggcct ctataaccag ccgttcgagg gacatgtggc
      601 cgaagacggc atctattttt catggaatca tttttttcgg gaatatgccg actattccga
      661 tggacagaat gtaggtctgc atgaaatggc gcatgccctc acctatgtca attttacggt
      721 gcaggaaggc cgcgacagta ctttccacga ccatttcatc gaattttccg ccgtcgcccg
      781 acccatcttc cagaggatgc agacggggga gaccacgctt ctcgatcctt atgcagcgac
      841 gaattaccag gaattctggg ctgtttgtgt agagacgttc ttcgagcgga gcaattcgat
      901 gaaaaagcag atgccggagc tctattcggc gctttgtata ctcctgaatc aggatccgct
      961 tacggcggat aagctgctga ctgtcgagac gggtgcggta tcgctgccgc cgtcgcctgc
     1021 cccggctaac ctgggcccgg caggtccttg attttcaaaa ttttatgaat ctgttgacaa
     1081 aactttcggc ggaactcttt tctttttcag gaattctttt tattttgtct tcctatccgt
     1141 acgattcatt tcctcttgat taaacccact ggtcggtttt ccattctaat ttgaaagcca
     1201 tagttccgta tccaaagcca atgacagacc tggtcgcaag ccatcatttc ttgaactaca
     1261 aagaaaaatg agcccttatg gaaatcgtcg tcatcttttc attggtattg ggaatcttgc
     1321 tccttaatcg cccggtaact gtgctttttc aacgtcttta cacgcggcat cagttcaaac
     1381 ggttcatcgc aagcgagact ttctaccacg ccgtcgtgtc ccgtcacttc cgttattata
     1441 cgcgtctcgg tctcgacgag cagcggaaat ttcttttccg cacctatctg tttcaacatt
     1501 ccaagaattt ccattatatc gaggtcgagc agtcggccga aatgccgatc ctcgtcagcg
     1561 ctgccgctgt acagctgacc ttcggtctgg aaaaattcaa gctcaattac ttcgacgata
     1621 tctttatcct gcgggacgac tatcactatg gcttctattc ccgtcctttt atgggccatg
     1681 tagaccagac cggcatctat ctttcctggg ataacttcat gaagggtatc accggtcaga
     1741 ctaccaactg caatgtcgga cttcatgaaa tggcccacgc cctcgcctat gtgaatttta
     1801 tcacccgcac ggaagaagac aaacatttta aaagggagtt tcccaacttt tccaaggtgg
     1861 cccgccccat cttcacttcc atgcaggagg gcattaaaaa tctgctgggc gactatgccg
     1921 ccaccaacta tcacgaattc tgggccgtag ccgtggagat cttcttcgaa agctcggttc
     1981 aattccgcca cgagctgccc gagctgtata aggcgatgtc gggcgtactc aaccaggacc
     2041 cattgggttg cctcacgggg agtacgacga tacttgtccg ggccgagagc gcgcctgtgc
     2101 agatggccgg tgctgaaatc accgcgtaag gtttgacctc aatgtctccc caggactttc
     2161 gagaagcgcc gcacatccgc ctccggaagg ataggggtgc cggcagtaat atagcggacc
     2221 agctggttcg cgaaataggg ggcgagcgag cagccttttg tgcccatgcc attgaggatg
     2281 ccgaccgccg ggtgggtggg atgaaagccg acgaaggggc gccgctcgag agtcgcgggg
     2341 cgcaccgatg caagatgctg gagggtgcgg aaaggcagct tcagccattc gcgcaggagg
     2401 gtctcggtgc ggtggcggaa tgcgtccgtt ggctctccat gaccgaaagc ccattcatag
     2461 gatgagccga cccaccaggt cccgtcacgc cagggggcga gactgattcc cctcttaaag
     2521 attgtttttt ggccggcaag acgctccgcc aggccttcga tcgacacgac cagcgcctcg
     2581 cctttattcg gtgcgaaagg gaggcgcgag aaccaggggt cggaagcgcc ttcgatccca
     2641 tcgcagaaaa tgatcttgcg cgcttcgaga tctttataca ggaccatgtc gccggacacg
     2701 aggatcagtt cctcgcgttc gaaccgctct tcgcgcagaa accctttttc cagcatccgt
     2761 cgccgcgcgg catccagtag gccggggaga tccacgagat gacagggact gatcactcca
     2821 aacccgaagt ccgaacgaaa gacatcatcc cagccatgct cgtcggtcgg catccggaga
     2881 taagacgcgt tctctccgag ccgttgtaaa aaagccagcc gcatctgtgg ggtgggaaaa
     2941 aaatccacca cggtggccgt ttcgaaaaaa gacgacccca gcagcccggg caaacgtcca
     3001 taggcgtcgc gcgcgaagga gaggagctct tcgatcatcc acgtggtgac gatacgtcgc
     3061 ccggtgacgg ggttgatcag ccctgccgcc gccctgctcg cgctgagggg acgtttgtca
     3121 tcgagaatga gatgcgaaat tcctgcccgt tcgagttcga ggctcaaaaa ggttccgcag
     3181 atgccctgcc cgatgatcaa tatgtcggtt tgttgtctca cttccgctgt atttagccgg
     3241 atcgtcgccg gatcgccgcc ggatcatcgc cggatcatcg ccagatcatc gccggatcgc
     3301 cgccggatca tcgcccgatc gccgccggat tgcgtgctca gttgcctgtc tccacattta
     3361 ctcgaatacc ctcggtgtgc caggtgaact cgggagcata caggcattcg atagtggtga
     3421 tcccattgct gaaattcccg gattgccccg ccagcatatc atactcgaaa acataggtcc
     3481 cgcgaggcaa tacgtcgaaa aagaaatccg tgctcgcgtc cttcgtgctt tcataatagc
     3541 ccagaccgtc ctgccatttg tactggctga gcacgttcac cggctccaaa caggctgccc
     3601 gcatatcctt catgtgcacg tattccatgt ccctgtccgc ccggagctcg atccgcgcga
     3661 cgaccctgtc accgggtttc agctcgccat tgtccgccaa tggctcgagc agaggtcccc
     3721 ggtccgtatt ccgccggatg aataattttt tcataacgct aagcgcgggt tttgcgcctc
     3781 ccggcggcgt gatcctgttg gacatatcga aatattgcca gtagaccgcg ccccaggccg
     3841 gggaaccgcc acctcctttc gtctccatgg atacattgat attccccatc gtggggttca
     3901 cgaacgcacc gtcgaaaact tttttattgt atccgacacc tgcatctccc gagccggccg
     3961 gccaatcgat ggtcttgtcg ccgagccgca tgtccacgga tcgctccgcg ttcaaccagt
     4021 tgtccccgcc catcagcagg gcgtaacagg cgtcggcggt tgccgtcgtc gtcggccagc
     4081 tgtgcgtttg tttttgtttc agcagccagg ttttgagatc ccggtcgata gccgcgtcgc
     4141 ccgatatctc gtgaaaggct tcgatcagca gggattgcgt ttccacgggt gcctgccacc
     4201 agtaatagcc gccctccatt cccttccaat acatcccgcg ttcggggtcg cggctcgcgg
     4261 tttgcttcag cgaggcgatg atatccctgg cggtctggat gtcgccggtg cgatagagtg
     4321 cgagtgcgat cattccctgc agatacggac tgcacttcaa ccagtcccgc tgcgccttct
     4381 tccggaaata atttactgct gcaaatgcgg ttcccggcag accgctgttg gcgaagaagc
     4441 tgcgcatgta gagataatgg acctgcatag ggccgatgag gtcctccgca ggacgcgctt
     4501 tcacgcgatg atagtcctta tttatctgct catccaggta cggtaacgcc gccgcgacga
     4561 tcgtatccag ccggcccgcc agatcgggcg acacggcccg caaccttcgc aaatgaccga
     4621 tccctgtcag gatatattgc gtgatatggc gattgtcggg tccaccggac caccagggga
     4681 accctccccc ttcggactgc atcttcgaaa gccgatcgat ggccgcgcgc agctcggtgc
     4741 tcatccggtg catatcgaac aacaacgcga gattcttttt ttgctgcgcc tcgctcttgc
     4801 cttgcatgac ccacggggtc tcctcgagga gcactgcttt cagctcctgg ttcttttgca
     4861 aattgcttag cagctgcgcc gtatctgtcc cgcgccaccg gtcgaacata tcttttaacc
     4921 gcggtgaggc acccgcaata tggctggcca gcgcattcgc atagacgcgc tcgaacacct
     4981 gttcggaaca ttcatgggga tattccatca ggtagggcag cgactgcacg gcgtaccatg
     5041 ccggattggc cgtgaattcg acggtcaggg catgatgatt cagcgtctcg ctgctgccgc
     5101 ttttgagcag cttgtcgaac gtgaaatgac gcgtcccgtc tcccggcata ttcaacggca
     5161 gactttcggt gaccattatg cgattgctga cgaccggtag tgtcgcctcc tcaccgtcac
     5221 tataggcgcc cgccgctgcg actaccttat acgtcaccgg ccggttgtat tgataaggga
     5281 tgtcgagagg aaaacttacc accacgcttt gcctcgcgcc gaccgtaaaa tactgattgg
     5341 cctgccggtt ggtgaaccag ccatccgccg tctcgcccgt cgtcggatcg gtcagcgaca
     5401 aattcatctg ccccgtcaat tcgctatcgg tcagattgac caccttcacc ggcagctcca
     5461 ttctatcacc ctcgcgcagg aatcgcggag cgttgggctg taccatcagc tctttttgcg
     5521 tgatgatggt tttttcgcca tacccgaacg ccagatcgcg cgtgtgcgcg agcgtcatcc
     5581 atttccagct ggtaacggcc tcgggcatcg tgaaggagaa gctgacattg ccggcgctgt
     5641 cggttcgcag gtccggcaaa aaaaaggcgg tttcccggaa atctttgcgc gcctggacgg
     5701 cgggcggagg ggcagtggca gaggatggct gcgggctttt gccggtattg actacgattg
     5761 agtccttcat gagtaatttg gacccatccg cgtctttttc catatccggc acctgcatgg
     5821 ccatgtaacc agccgaaacg tcaaatttcg ccatcccggg agcaggaccg gtgcggaagg
     5881 acatccgccc ctcgagcagc cgctctctta tttcctgatt atcgacagac aataatctat
     5941 cgtacaccgg gaagaaatac cgaatgtcgc tgccatatgt atttcgccgt tcggatctat
     6001 ccgaagcaaa atcgatcccg ttccatttcc agaaaggctc gaatacctcg tacagatgcg
     6061 gtaccaccca tcggtgtggt ttaaattgat cgagtgatgc atcgtacatg cctgccagta
     6121 cctccgaggc aactttctca gcccgcgatc cttcaattgt cacctcccag ttttccaggc
     6181 ttccgggcaa agtcttgtcg cggtaactga gataacggat gctcagctcc ttattcgtcc
     6241 agggcacatt gatggtggac aaccgggtgt aaattctatt gtcctttatg aacacgtccg
     6301 agacccccag gccacccctg tcggcctcgg tcaccggaat ctccacttgt ttccggtctc
     6361 tatccagggt caaaaagcga aatgatctgt tctgactgac ctcctccatc cgttcgacgg
     6421 cccggatcac gaatacgctc ccggctgaag acccgatgga taccaacgcc cgattcccgg
     6481 gttccgtcgt ttggtccgcc tgcatggtcc aatcgtacaa aggtcgggca ggctttccag
     6541 tcgagccgtc atataattca acgtatcgtt cggtttttac cggctgacca ttcgcatcgt
     6601 gtgcgattgc ttcgatgata taccagccgg gagccaatcc ctttccggaa atgcccgtga
     6661 cagagattga ttttcccgcc gagtcggtgg ctccgaatac tttttgcccc agctgccagc
     6721 ttttcggatc ggattcgtct ccgtattcat catggggaaa atcatggaga aaagcctctt
     6781 ttgtcagaat gaactggtcg ggcgccggcc aataccgttc ccggatcagt cggttcggag
     6841 actgcaagcc gtaaattgaa atctctacca acaccggctg ggggataccc gcaagattaa
     6901 ccgcatttac cacgatcata ttgcaactgt cggcagatat atgatcctcc acaggctgac
     6961 cgatcgtcag ttccagtccc cggtatccgg cttttacact gccctggctg gtatgggttt
     7021 ctccgctgat atccgtaaca tcggcggaaa tggtgtattc aaaaatgggc tcgtaccctt
     7081 tctgaatact tttatcgggc tttgccttaa aagagatgcg gaaccttcca tttgcatcgg
     7141 ttctgagagt tccatgcgag atctcatcgg ccgatctgtt gtatgaccat cggttccagg
     7201 ccaattgcct ggtcacccgg tattttatca acgcaccatc gattccgttg cccgcatatg
     7261 ctttagcgta tccttccatc cggatggtat cacctatccg atagtttgtt ttcagtgtat
     7321 cgaaagagac atagaatttt ggccgtttgt attcctcgac ataaaaagcc ttagactgat
     7381 cggtagagtc gtcctgtagc ataaattggc cgttgagcaa cccttgcgga agggtaaacc
     7441 ggccatgata agacccgaat tcattggtgg tcactgctat cgaatccacc tttgttccat
     7501 tcgcatcgat caaaaatacg cgcgtatgga accctgctcg tattttgacc cgccgggtac
     7561 tgaaatcgcg ggaagtaacg attcctttga aatagagagg ctgccccgga cggtagatcg
     7621 accggtccag gaatatccag gtggtcagat tatccttttc gtatcggacg ctgtccgatg
     7681 atcctctgct atttccccgt acat
//
LOCUS       DASYSV010000453         4297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_92167, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000453 DASYSV010000000
VERSION     DASYSV010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92167"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1566)
                     /locus_tag="VGQ51_13915"
     CDS             complement(115..1566)
                     /locus_tag="VGQ51_13915"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9037725.1"
                     /translation="MIRGWAMYDWANSVYNLVITTTFFPIYFIESTRHQFKSDVIPFL
                     GMHISNSALYSYALAAAYLLIALLYPILTSIADTRGNKKSFMRFFCYMGALGCSLLYF
                     FDGANLGLGIVCFMLASMGFVGSLVFYNAYLPEIAAPLDQDRVSAKGFTFGYIGSVIL
                     QVIGFAVVLLTQDKDAFIGPRITFLLVGIWWAGFAQITFSVLPKSPVTATVTRTNVLR
                     TGFREVAKVFAEVKKMAVLKRYLRAFFFYSMGVQTVMSAATLFGDKLLRLDATKLIIT
                     IVLIQLVAIAGATWMSRLSSRYGNIRVLMGVVVFWVMICVSAYITAGWAEGLKGFHDR
                     IDDLKQEKEELVSKRSQLPGDEFDRKDASLEAELSHAELELEPHQQPIEYSFYVLALG
                     VGLVMGGIQSLSRSTYSKLMPDTKDTASYFSYYDLTEKIAIVVGMFSFGLIEEVTGSM
                     KNSIIFLIVFFVIGFMWLYGALLKQRVSTAAAV"
     gene            complement(1635..2432)
                     /locus_tag="VGQ51_13920"
     CDS             complement(1635..2432)
                     /locus_tag="VGQ51_13920"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV9037726.1"
                     /translation="MTITQAIIQSVVEGLTEFLPISSTAHMKFVNPMIGVDPNDDFSK
                     LFDIIIQLPAILAVVVLYWRKFFDFSRISFYVKLIVAVIPALIFGALLGKYIDRALDN
                     LYFIAIVMVSGGIILLFVDKWFTRPIIDDEQKVTNGIAFKIGCFQVLSILFPGLSRSA
                     ATIVGGMTQKLTRKAAAEFSFFLAVPTMIAATAKKLWDAHKEHPDLLNREHATTLALS
                     SIVTFMVAIIVIRFFIGAIKKYGFKPWGVYRIVAGGVMLFLLWRGML"
     gene            complement(2439..2741)
                     /locus_tag="VGQ51_13925"
     CDS             complement(2439..2741)
                     /locus_tag="VGQ51_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HEV9037727.1"
                     /translation="MATTKKPAASATAPAKAKPAATHPQTPLFGKENYRWMLIGLIAV
                     ALGLILMGGGKSKDPNVFNPKEIYSFTRITLAPILILGGFVIEIFAIFRKASKSAQ"
     gene            complement(2777..3649)
                     /locus_tag="VGQ51_13930"
     CDS             complement(2777..3649)
                     /locus_tag="VGQ51_13930"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEV9037728.1"
                     /translation="MAQIGKGSAKRSTPSYFMAIVGVSLVLFLLGLLGWIVINANKVG
                     ETFRENVEVQVYVRENISPKDSAALVQLVASRPYVKDFKYVTKDMARKEFVGTGNDDF
                     MSVLTQNPLPASVDFRLRSEYANADSLNRIVADFSGNIAVDEVKYNKKLVTNLNDTIR
                     KVSLVLLIVTLVISIAVIVLIDNTIRLAMFSNRFLIKTMQMVGATRWFIAKPMDLRAI
                     VNGAVSGLIAIAAIIGLIFVAEAWIPEAKALRDYVLLSGMFFVMILLGICISFISTHR
                     SVVKYLKMKLDDLY"
     gene            3816..>4297
                     /locus_tag="VGQ51_13935"
     CDS             3816..>4297
                     /locus_tag="VGQ51_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV9037729.1"
                     /translation="MEYNFGEIEKKWQEWWTSNGVYKVSNDPSQPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIFSRYKRLKGFNVLHPMGYDAFGLPAEQYAIDHGIHPAVSTDD
                     NINNFRSQLDNIGFCFDWDRSFRTSDPGYYKWTQWIFLQLFDSWYDKHQNKAVPIEWL
                     V"
BASE COUNT         1075 a         1170 c         1087 g          965 t
ORIGIN      
        1 ttgggaacca cgccgaacat ggcgccgccg tccaatttaa aaaagccggt attgatcgta
       61 taaagttgca tacactataa ttcgtgtcgc ctaccgcgcg gaggccctgg cggctcacac
      121 cgctgcggcg gtactcaccc gctgtttcaa caaggctccg tacagccaca taaacccgat
      181 cacaaagaaa acgatcaaaa aaatgatcga attcttcatg ctccccgtca cctcttcgat
      241 aagcccgaag ctgaacatgc cgacgacgat ggcgatcttt tcggtcaggt cataatagct
      301 gaaataggaa gccgtatcct tcgtgtccgg catcagtttg gaataggtgc tccggctcag
      361 cgattggatg ccccccatga ccagaccgac gccaagggcc agtacataga aagaatattc
      421 gatcggctgc tgatgtggct cgagttccag ctcggcatgc gacagctccg cttccaggct
      481 ggcgtcttta cggtcgaatt catcgccggg cagctgcgat cgtttagaca ccagctcctc
      541 cttttcctgc ttcagatcgt ctatccggtc gtgaaaaccc ttcagaccct ccgcccagcc
      601 ggcggtaata taggcggata cgcagatcat cacccagaag acgacgacac ccatcaggac
      661 gcggatattg ccataacgcg aagagagcct gctcatccat gtggcgccgg caatggcaac
      721 cagctggatc agcacgatgg tgatgatcag tttcgttgcg tccagtctca ggagcttgtc
      781 cccgaacagc gttgccgccg acatcaccgt ctgaaccccc atgctataaa aaaagaaggc
      841 cctcagataa cgcttcagca ccgccatttt ctttacttcg gcaaatacct tcgccacttc
      901 gcggaaaccc gttcggagaa cattggtccg ggtgaccgtc gccgtaaccg gagatttcgg
      961 cagcaccgaa aaggtgatct gcgcgaatcc cgcccaccag atgccgacca gcaggaaggt
     1021 gatccgcggt ccgatgaacg catccttatc ctgcgtcagt agcaccacgg caaacccgat
     1081 cacctgcagg atcacactgc cgatgtaacc gaaagtgaaa cccttggcgc tgaccctgtc
     1141 ctggtccaat ggcgctgcta tctccggcag gtaggcattg tagaaaacga ggcttcccac
     1201 gaagcccatc gaagccagca tgaaacaaac gatgccgagc cccaggttcg ctccgtcgaa
     1261 aaaatacagc aggctgcacc ccagcgctcc catgtagcaa aaaaatcgca tgaaactctt
     1321 cttattcccg cgtgtatcgg caatggaggt gaggatgggg tagagcaggg cgataagcag
     1381 gtaggccgcc gccagtgcat agctgtacag cgcgctattg ctgatatgca tcccgagaaa
     1441 tgggatcacg tcgctcttga actggtgacg tgtggattcg ataaaataga tcggaaaaaa
     1501 ggtcgtcgtg ataacgagat tgtatactga attggcccag tcatacatgg cccagcctct
     1561 gatcactttt ttgggggccg tttggacaaa tatccctgtc aagggacgcc cctcggcttc
     1621 aggcccccgc ccggtcataa catacccctc cacaaaagga acagcatgac gccgccggct
     1681 acgatacggt acactcccca gggtttgaac ccgtattttt tgatggcccc gataaagaac
     1741 cggatgacga tgatggcgac catgaatgtc acgatactgc ttagcgcaag cgtcgtggca
     1801 tgttcgcggt taagcagatc gggatgctcc ttatgcgcgt cccacagctt ttttgccgtt
     1861 gcggctatca tggtaggcac tgccaggaaa aaggaaaatt ccgcggcggc cttccgggtc
     1921 agcttttggg tcataccccc gacgatggta gccgcgctgc ggctcaatcc gggaaaaagg
     1981 atcgacagca cctggaaaca gccgatctta aaggcgatgc cattggtcac tttttgctcg
     2041 tcatcgatga tcggccgggt gaaccatttg tccacgaata gaagaatgat gccccccgaa
     2101 accattacta tggcgataaa atagagattg tccagcgcgc gatcgatata tttccccagc
     2161 aatgccccga aaataagcgc cggtatcacg gcaacgataa gcttgacata aaagcttatc
     2221 cgtgaaaaat cgaagaattt acgccaatat aaaaccacca ccgccaggat agccggcagc
     2281 tggatgatga tgtcgaaaag ttttgaaaag tcgtcgttgg gatcgacccc gatcatgggg
     2341 ttcacgaatt tcatgtgcgc cgtggaggag ataggaagaa attccgtcag gccttctact
     2401 acggactgga taatggcctg ggtaatggtc atgtcgggtt attgagcgct cttactggct
     2461 ttgcggaaaa tggcgaagat ctcgatcaca aaaccgccca ggatcaggat gggggcgagc
     2521 gttatccgcg taaagctata tatctccttg ggattgaata cattgggatc tttgcttttc
     2581 cctccgccca tcaggatcag acccagggcg acagcgatga gaccgatcag catccagcga
     2641 taattctcct taccgaacag cggagtttgc gggtgcgtag ccgcaggttt agccttggcg
     2701 ggggcagtgg ccgaagcggc tggtttttta gtcgttgcca tagatgaata gatgattggg
     2761 cctgcaatat acacaatcag tacaagtcgt caagtttcat ttttaggtac ttcacgacgc
     2821 tgcgatgcgt actgataaag gagatacaga tgcctagcag gatcataacg aagaacatcc
     2881 cactcagtaa gacatagtcc cggagcgcct tcgcctcggg gatccacgcc tctgcgacga
     2941 agatgagacc gataatggct gcgatggcga tcagcccgct gactgcccca ttgacgatgg
     3001 cgcggaggtc catgggcttg gcgatgaacc accgggtggc gcccaccatc tgcattgtct
     3061 tgatgaggaa acggttgctg aacatggcca gccggatcgt attgtcgatc aggacgatga
     3121 cggcgataga gatcaccagc gtcacgatca gcaaaacgag gcttacctta cggatcgtat
     3181 cgttgagatt ggtcaccagt ttcttgttgt acttcacttc atcgacggca atattgccac
     3241 tgaaatccgc gacgatccgg ttgagtgaat ccgcattggc atattcgctt ctgagccgga
     3301 aatctacgct ggcgggaagg ggattctgtg tcagcacgga catgaaatcg tcatttcccg
     3361 tacccacgaa ttcttttcgt gccatatcct tggtgacata tttaaaatcc ttaacgtagg
     3421 gcctggacgc aaccaactga accagggctg cgctgtcctt cggtgagatg ttctcccgca
     3481 cgtatacctg cacttccacg ttctcccgga atgtctcgcc taccttattg gcattgatga
     3541 cgatccagcc cagcagcccc agcaggaaca gcaccaggga aacacccaca atggccatga
     3601 aatatgaagg agtcgaacgt ttggcagaac cttttccaat ttgcgccatg agtaagccgg
     3661 ttttattgat caaaaatagg gctcaatttt gtatttttgt tacccctcca aacctgattt
     3721 gatccggttt gacaaatatt attgggttta ttaacggttc gttgcccgca ttattacgat
     3781 aatacacaag gttttaactt ttttccaaca taggcatgga atacaatttc ggcgaaatag
     3841 aaaaaaaatg gcaggagtgg tggacgtcca acggcgtcta caaggtatcg aatgacccct
     3901 cccagcccaa atattatgtg ctggacatgt tcccttatcc cagcggagcg ggtctccatg
     3961 tgggccatcc cctgggctat atcgccagcg atatcttctc ccgttacaaa cggctcaaag
     4021 gcttcaacgt cctccatccc atgggctatg atgccttcgg tcttcccgct gaacaatacg
     4081 cgatagacca tggcatccat ccggccgtca gcaccgatga caatatcaac aatttccgca
     4141 gccagctgga caacatcgga ttctgtttcg actgggaccg ctcgttccgc acctcggacc
     4201 cgggctatta taaatggacc caatggatat tcttgcagct cttcgacagc tggtacgaca
     4261 agcaccagaa caaagcggtt cctatcgaat ggttggt
//
LOCUS       DASYSV010000454         5662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2270345, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000454 DASYSV010000000
VERSION     DASYSV010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2270345"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VGQ51_13940"
     CDS             <1..466
                     /locus_tag="VGQ51_13940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV9037730.1"
                     /translation="LSLSSKKDSTVNDKRLLRQNQGDIFSVGYVTYKVLTSTEKVVYR
                     ASYIFLDGSSLSNSQIDSLKKIIVAKANSGTPWGQLSDQYTMDGNTTHGDTGWFFGEE
                     MMPKEFQDAVKEHNTGEIFFVDVPDKQWHYIVKKTYDDDDKKEMTVLRSNGR"
     gene            complement(470..2488)
                     /locus_tag="VGQ51_13945"
     CDS             complement(470..2488)
                     /locus_tag="VGQ51_13945"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV9037731.1"
                     /translation="MQRIFPSPAILRSSITVCSIVLATLRLSAQNSLNVFVSSWQPSN
                     TVQTYLIQEVHRQYDRRRADLQRALSSPAAMTAYRDECRHRYLQLLGAFPEKTPLHAQ
                     VTGSIEQPGYRIEKIIYESLPGHHVTSDLYLPSGKGPFPGILLFCGHEPESKAYAAYQ
                     RTAILFAKNGFVVLAIDPISQGERYQMTDQQGRRVMMGGGEHILFNADGLLAGTGAVA
                     DELWDNVRSLDYLVSRKEVDSSRIGCLGNSGGGTQTTYFAGFDPRIKVSAPCSYFASR
                     ERNLYYMGASDGCQHIPGEGENKLEIADFMIMAAPKPTLILAGKFDFVDDVGVRIAFS
                     ELQHVYSRLGYPDRCRLFVYDDGHGISKPKREIAVSWFKKWLCHDSTPVKEGDLPILP
                     EKDLFCTSTGQVNTAFPNERTLKDIDLSNANRFKTTRDAFAGRSDKQDIIRRVLHLGK
                     MDNTSISVEWTGEASSGTVNFKKAIVRKKGEIPLPCLVAYPQGPVKSICVWTVSEGKS
                     RLAKDSIALIESQLGSGNMVILSDLRATGETADNPGLSSSNYYFNKEYRNIMTALHVG
                     KPMVGQQVTDMITLLDFITEPEGLGLDGRPIDIHAEGGDAVVALHCLALDRRISVAHL
                     FHTIRSYYDLLNDLTQKDQYQYLLPGVLKYYDLPDLAKMAGPGKVFYE"
     gene            2570..3223
                     /locus_tag="VGQ51_13950"
     CDS             2570..3223
                     /locus_tag="VGQ51_13950"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEV9037732.1"
                     /translation="MDHSSIVKRLEGAYSGAVFDVLRGMGYHHQSLPSSIHPLNSIQK
                     LAGPVYTIEGRRDDTLSDHETLLRWCQLLSNAPAGYILVCQPNDSTVSHMGELSSETL
                     AYKSVKGYIVDGGCRDSAFIEKLGFPVFCRYYTPVDIVGKWAATGFGEPIRIGEVTIR
                     TDDFVMADRDGVVVIPREIVEEVTTKVEEVLATENKVRTAILAGTDPVDAYLRYGKF"
     gene            3227..3505
                     /locus_tag="VGQ51_13955"
     CDS             3227..3505
                     /locus_tag="VGQ51_13955"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEV9037733.1"
                     /translation="MGTIRLHSDDNVVVCIRSLAAGEEVLIDGHLTKMPALLGIGHKL
                     AWRDIRAGDPIVKYGIVIGTATTDIARGGHVHTHNMKSNYIPTYLNEE"
     gene            3511..4713
                     /locus_tag="VGQ51_13960"
     CDS             3511..4713
                     /locus_tag="VGQ51_13960"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEV9037734.1"
                     /translation="MENEQISGYLRSDGRMGIRNIVLIAYLVECAHFVADEIASGFKG
                     QPVHVIGFSGCYPNQHADKVMNALCCHSNVGAVLLLSLGCESFNKQHLFKTIHSSGRL
                     VHTITIQQAGGTRSSVKKGSEWVSTALETLSRQPRLPMRISELIIGTICGGSDATSGI
                     TANPAVGLASDKLVSTGATVLFEETGEMIGLENDMAARAVTKELGIELYDAVRKAARY
                     YTIMGHGSFAPGNADGGLSTIEEKSVGAYSKSGHSPIAGMVKPGDIPPQGGLYLLDLV
                     PDGEPRFGFPNINDSAEIAELMACGCHIILFTTGRGSVVGSAIAPVIKICANPETFRR
                     LPEDMDVNAGKLLAGEAGLEEISLEIIDRIVDTAGGSPTCSEALGHREFVLSYKSFGP
                     LGPSCLPL"
     gene            complement(4767..>5662)
                     /locus_tag="VGQ51_13965"
     CDS             complement(4767..>5662)
                     /locus_tag="VGQ51_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9037735.1"
                     /translation="SVASNEKLKHACPIVYAPSNLTVNRSPSGLTLKWNASTTTGVTY
                     SIYRGWTNGTETFFKAGITDTTFLDSSVSAGVGYWYFVKTVISDCDSTPSGEAFKYAC
                     QTVAAPTNLTETAQTGKIILTWNASTTPRVTYSVYRGWLPGSETWYAGGLTDTTFTDN
                     NVTSGTGYWYFVEAVLSDCDSAASAEKLKYALATSPGSVSGNGAPFNDMDSTGDLRLS
                     IYPNPSHGEITIALFLPQSAGVNLSIWNAQGQEIGQLTDGVLSQGAHTFTFHGGALPK
                     GVYFFKMRVGNQVTTHKILIQ"
BASE COUNT         1269 a         1406 c         1622 g         1365 t
ORIGIN      
        1 gctcagcctg tcctccaaaa aagattccac tgtcaacgac aagcggctgt tgagacagaa
       61 ccagggcgat attttttcgg tgggctatgt aacctacaag gtgctgacct cgaccgagaa
      121 ggtcgtctat cgtgccagct atatctttct ggacggaagc tcattatcca actcacagat
      181 cgacagtctt aaaaaaataa ttgttgcgaa ggcgaactcg ggcactccct ggggtcagct
      241 gtccgatcag tatacgatgg atggtaatac cacgcatggt gataccggtt ggttcttcgg
      301 cgaggagatg atgccgaagg agttccagga cgccgtcaag gagcacaata cgggcgagat
      361 cttcttcgtg gatgtgccgg acaaacagtg gcattacatc gtcaagaaaa cctacgatga
      421 cgatgataag aaagagatga ccgtcctgcg gtccaatggg cgatagcggc tactcataaa
      481 acacttttcc cgggcccgcc atttttgcca gatcgggaag atcgtaatat ttcaggaccc
      541 cgggcaaaag gtattggtat tggtccttct gtgtcagatc gttcaaaaga tcgtaataag
      601 accggatggt gtgaaacaga tgagcgacgg atatccgccg gtccagcgca agacaatgca
      661 gcgccacgac tgcgtctcct ccttcggcgt ggatatcgat ggggcgacca tcaagtccga
      721 gtccctccgg ttcggtaata aaatccagca gagtgatcat gtccgtgacc tgttggccga
      781 ccatgggttt gccaacgtgc agcgctgtca ttatattccg gtattccttg ttgaaatagt
      841 agtttgaaga gctgagtccg gggttatccg ccgtctctcc cgttgcccgc aggtcggata
      901 atattaccat gtttccgctg cctagctgcg actcgatgag cgctatgctg tcctttgcca
      961 gtctggattt tccttctgag accgtccaga cacatattga tttcacgggg ccctgtggat
     1021 atgctaccag gcaaggcagc ggtatctctc ctttttttct aacgatggcc tttttgaagt
     1081 taacggtacc ggatgaagcc tcgccggtcc attctaccga gatggatgtg ttatccatct
     1141 tgcccaggtg cagcacgcgc cggatgatgt cctgtttatc gcttcgtccg gcaaaagcgt
     1201 cgcgggtggt cttgaaccgg tttgcgttgg acaggtcgat gtccttcaat gtccgttcgt
     1261 ttgggaaagc cgtatttacc tgacctgtcg aagtgcagaa taagtctttt tcgggtaata
     1321 tgggcagatc accttcttta accggggtgc tgtcgtggca gagccatttt ttgaaccagc
     1381 taacggctat ttcacgtttg ggtttcgaga tgccgtgacc gtcatcataa acgaaaagcc
     1441 tgcatctatc gggatatccc agccgggaat atacgtgctg tagttccgag aaagcgatcc
     1501 ggacgcctac gtcatccacg aaatcgaact tgccggccag gatcagggtc ggtttgggtg
     1561 ctgccatgat catgaaatcc gcgatctcca atttgttttc accttcgccc gggatatgct
     1621 gacatccgtc gctggcgccc atgtagtaca ggttgcgttc cctgctggca aaatagctgc
     1681 aaggggcgga gacttttatc cgggggtcga aaccggcgaa ataggttgtt tgcgtaccgc
     1741 cgcccgaatt tcccagacaa ccgatacggg aactgtccac ttctttgcgg ctgaccaggt
     1801 aatcgaggct gcgcacattg tcccacagct cgtccgcgac ggcgcctgta cccgcgagca
     1861 ggccatccgc attgaaaagt atatgttcgc cgccacccat catcacgcgt cggccctgct
     1921 gatcggtcat ttgatatcgc tcgccctgcg agatcggatc gatcgccaat acgacgaaac
     1981 cgttctttgc aaaaaggata gccgttcgct gataggcggc gtaggctttc gactcgggtt
     2041 catggccgca gaacagcagg atcccgggga atgggccttt tccggacggg aggtacagat
     2101 ccgaggtcac gtgatggccg ggcaggctct catagatgat cttttctatc ctgtatcccg
     2161 gttgttcgat cgaacccgtt acctgcgcgt gtagcggtgt cttttcggga aatgcgccca
     2221 ggagctgcag atagcggtgt ctgcactcat cccgataagc cgtcatggct gcagggcttg
     2281 acaatgcccg ttgaagatcg gctcttcgcc ggtcgtattg ccggtgtact tcctgtatca
     2341 gataagtttg aaccgtattg gagggctgcc aggaggatac gaatacgttg agggagttct
     2401 gtgctgacag tcggagtgtt gccaatacga tcgaacaaac ggtgatcgat gacctaagga
     2461 tggccgggga aggaaatatg cgttgcatag gagtaaaagt aaaaaattta tctgtattgt
     2521 actatactat accatttttt ttctactttt aggtcataaa ccacaatata tggatcacag
     2581 ttctattgtc aaacggttgg agggagctta tagcggggcg gtattcgatg tgctccgcgg
     2641 aatgggttat caccatcaat cgctgccgtc ctccattcac ccgctgaata gtattcagaa
     2701 gctggcgggt cccgtttata cgatcgaggg gcggcgtgac gatacgctta gcgatcatga
     2761 gacgttgttg cgctggtgtc agttgttgtc caatgcgccg gcggggtaca ttttggtttg
     2821 ccagcccaat gacagcactg tatcgcatat gggcgaatta tcgtcggaga cgcttgcgta
     2881 taaatccgta aaaggatata tagtcgatgg cggttgccgc gactccgcct tcatagagaa
     2941 actgggtttc ccggtctttt gccgttatta tacgcctgtg gacattgttg gaaaatgggc
     3001 tgccacaggt ttcggcgagc cgatccggat tggggaagtg accatccgta cggatgattt
     3061 tgtcatggcc gacagggacg gtgtcgtggt gatcccgcgt gagatcgttg aggaagtgac
     3121 cacgaaggtg gaagaggtgc tggcgacgga aaacaaggta aggacggcga tactcgcagg
     3181 aacggatcct gtcgacgcgt atctccggta cggtaaattc tgatcgatgg gtacaatcag
     3241 acttcatagc gacgataatg tcgtcgtctg catcaggagc cttgcggccg gagaagaggt
     3301 cctgatcgac gggcatctga cgaagatgcc ggcgttgttg ggcatcgggc ataaactggc
     3361 ctggcgggat atacgggcag gagaccctat cgtcaaatat ggcattgtca tcggtacggc
     3421 gacaaccgat atcgcgagag gcggccacgt tcatacccac aatatgaaaa gtaactatat
     3481 cccgacttat ctaaacgaag aataaaggct atggaaaacg agcaaatttc gggttacctg
     3541 cgcagcgacg gcagaatggg catccgtaat atcgttctaa tcgcttacct ggtggaatgc
     3601 gcgcatttcg tggcagacga gatcgcatcc ggttttaagg ggcaaccggt gcatgtcatc
     3661 ggctttagcg gctgttatcc taaccagcat gccgataagg tgatgaacgc gctttgctgc
     3721 cattcgaacg tcggtgccgt gctgttactt tcgctgggtt gcgaaagttt caacaaacag
     3781 catcttttca aaaccataca ctccagcgga agactggtgc atacgatcac gatccagcag
     3841 gccggcggta cacggagttc tgtaaaaaag ggcagcgaat gggtatcgac cgctttggag
     3901 accttatcgc gtcaaccacg gttgcctatg cggatcagcg aactcattat cggtacgatc
     3961 tgcggtggat ccgatgccac cagcggcatt acggcaaatc ccgctgtggg gcttgcttcc
     4021 gacaaattag tgtcaaccgg cgccaccgtg cttttcgaag agacgggtga gatgatcgga
     4081 ttggaaaatg acatggctgc acgagccgtc accaaagaac ttgggatcga attgtatgat
     4141 gccgtcagga aggccgcaag atattatacg ataatgggtc atggaagttt cgcaccgggc
     4201 aatgccgatg gcggtctgag cactatcgaa gagaagtccg tgggcgccta ctccaagagc
     4261 gggcattccc cgatcgcggg catggtaaaa cccggcgata tccctcccca gggaggtctt
     4321 tatctcctgg acctggtacc cgatggcgaa ccccggttcg gctttcccaa tatcaacgac
     4381 agcgccgaaa tagccgaatt gatggcttgc ggctgtcata tcatcctgtt cactacaggg
     4441 cgtggttccg tagtgggatc ggcgattgcg ccggtaataa agatatgtgc caacccggag
     4501 acgttcagac gacttccgga agatatggac gtcaacgccg gcaagctgtt ggcgggagag
     4561 gccggtctgg aagaaatttc tcttgagatc atcgatagga tagtcgacac tgccgggggg
     4621 agccctacct gttccgaagc gctcgggcac cgggaattcg tgttgtccta taagagcttc
     4681 gggccgctag gtccatcttg tcttcctctt taaaaaaaga gtcagcgcag aatgcgctga
     4741 ctcgcttgct ttctttcata gggtctttac tggatcaata tcttatgggt ggtaacctgg
     4801 ttgcctacgc gcatcttgaa gaaatagact cctttgggga gcgctccacc gtggaaggtg
     4861 aaggtatggg cgccctggga cagcactccg tccgttagtt ggccgatctc ctgtccctga
     4921 gcgttccaga tgctcaggtt cacaccggcg ctttgcggca ggaacagtgc aatcgttatt
     4981 tctccgtgcg atggattcgg gtaaatgcta aggcggagat ccccggtaga atccatgtcg
     5041 ttgaaagggg caccgttgcc ggatacgctt cccggggaag tagccagggc atatttcaat
     5101 ttctcggctg aggcggcgct gtcacaatcg ctgagaacgg cctctacaaa gtaccaatac
     5161 cccgtgccgg aagtcacatt gttatccgtg aaggtagtat cggtgagacc gccggcgtac
     5221 caggtctccg agccgggcag ccatccgcga tagaccgaat aggtgacgcg tggtgtcgta
     5281 ctggcgttcc aggtgaggat gatcttaccg gtctgtgccg tctcggtcag gttcgttggt
     5341 gccgctaccg tctggcaggc atatttgaat gcttcgccgg agggggtgct gtcgcaatcg
     5401 gaaatgacgg ttttgacgaa ataccagtaa cccactcccg ccgaaacgct gctgtccagg
     5461 aacgtggtgt cggtgatacc tgccttgaag aaggtctcgg tcccgttggt ccatcctctg
     5521 tagatggaat aggtaacgcc cgtggtggtg cttgcattcc atttcagcgt gaggccggaa
     5581 ggcgaccggt ttacggtaag gttggagggc gcatagacga tcgggcaggc atgtttcagc
     5641 ttctcattgg acgctacgct gc
//
LOCUS       DASYSV010000455         4320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1888889, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000455 DASYSV010000000
VERSION     DASYSV010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888889"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VGQ51_13970"
     CDS             <1..1171
                     /locus_tag="VGQ51_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV9037736.1"
                     /translation="PISWLHRSERYGEKLATPDVSVADLIGDIDPIKAANLRLTFADE
                     EVIHFGIIPKSNRGIFVINEIPDLQARIQVSLFNILEEGDIQIRGFKLRMPLDMLFVF
                     TANPEDYTNRGSIVTPLKDRIESQILTHYPKSIETALAITEQEADIHEEQKGRVIVSD
                     LVKRLIEQVSFEARGSEYVDKKSGVSARLTIAAFENAVSAAERRAILNNEKETPVWMS
                     DLVGIVPSITGKIELVYEGEQEGPYQVAFNLLEKAFRSQFVNYFPNPDQLKKRKSDSR
                     EDNPFRPITHWFDKGNHLNLFFNTPDHEKIQQLYKVDGLHALVKKYFKHANEREAALL
                     MEFVLHGLAAYSLISKKVVEGKIEFKDLIGSMVNLGSSGSSFGSEDEDLEGDEFN"
     gene            complement(1208..2197)
                     /gene="murB"
                     /locus_tag="VGQ51_13975"
     CDS             complement(1208..2197)
                     /gene="murB"
                     /locus_tag="VGQ51_13975"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV9037737.1"
                     /translation="MKNLNTFGIDARARYFSAFSSADELAGLLDWAGGRRKVVLGGGS
                     NILLTGDIDGIVLQNAIKGIELVDEDEEHVYVRAGAGEDWTRFVDYCIGRDWAGVENL
                     SLIPGSVGAAPMQNIGAYGMELENVFLELDAWSLADRNVRTFTLGDCEFGYRDSVFKR
                     HYRDQSVILSVTLRLRKKPIFHTGYGAVQDELDRMGVKELSIRAISQAVIAIRRSKLP
                     DPAQIGNAGSFFKNPIVAGDQFAQLQAKFPGIVGYPDPLGQRTKLAAGWLIEQCGWKG
                     YRKGDAGVHERQALVLVNYGNARGADIYRLGEEIAASVRDKFGVSLEREVNLL"
     gene            2311..>4320
                     /locus_tag="VGQ51_13980"
     CDS             2311..>4320
                     /locus_tag="VGQ51_13980"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV9037738.1"
                     /translation="MTRHLIACTLTLISFSLYAQENNPLIVSGQLIEQAGTLYDSGQY
                     KKAIDVFRRIDRNDTNYIRALYGISSCLYEDSQFSASLEYARKGLALTADPERRPDLY
                     NQIGNTLNESEASEQAIKVYDSAINKYPAYSILYLNKGSALIKLGRYPEAEAVFKQAL
                     LKDPYSYSSHYKLGYCAINQGKLVPAFLSFMGYLLLNPEGRYKSSCIRWMDAIAKNMD
                     TIQTFLVHRKDDAAGNYQLLEQILQSKIALDRNYKTMIQLDDPISRQIQVVFEKMNYE
                     PSDSDFWMQYYIPFFKSVFSNRQFEPFINRLFLGVDIPVIQEYHRRNKKEVQALLDAT
                     VTYFNHIRSTRELNYAQRDTASASWFFSNGELSSHGRYLVKEDRLLGNWNFFYNPGNI
                     RSTGTYNDNGKKEGWFNSYFFDGKPRGKEFYVNGEQEGEETFYFNSGTMSTHSWYKKG
                     QLEGEAVSYFWVGTPHTITHYHEGKEDGVKLTYRNNGDTALLEHYTSGTLNGEVRSWA
                     ADRTVEVIASYQNGELEGEYRKYYPNGQLYMDGNYKAGKQDGEWKSWYNNGQLKTVDH
                     FLNDKSEGEYREYHDNGTLRYTANQKNGKFVGEIRYFDDDGKVYSVQNYSNGRLQKAK
                     YFDKTGAQIGESESGGSGMELVDFLPDGSRRVQYTSDDKGNINGTE"
BASE COUNT         1117 a         1259 c         1061 g          883 t
ORIGIN      
        1 cccgatcagc tggctgcatc gcagcgagcg gtatggagaa aagctggcga cgccggacgt
       61 cagcgtagcc gacctcatcg gagacatcga tcccatcaag gctgccaatc tgcggctcac
      121 cttcgccgat gaagaggtga tccatttcgg tatcatccca aaaagcaacc gcggcatctt
      181 cgtcatcaac gagatccccg atctgcaggc gcggatacag gtgtctcttt tcaatatcct
      241 ggaagaaggc gatatccaga tcaggggttt caaactgcgc atgccgctgg atatgctgtt
      301 cgtgtttacc gccaaccccg aagactatac caaccgcggc tcgatcgtca ccccgctgaa
      361 ggaccgtatc gaaagccaga tcctgaccca ttatccaaaa agtatcgaga cggcgctggc
      421 tattaccgaa caggaagcgg atattcatga agagcaaaag ggtcgcgtca tcgtgagcga
      481 tcttgtcaaa cgtctgatcg aacaggtgtc attcgaagcc cggggcagcg aatacgtgga
      541 taagaaaagc ggggtttccg cacgccttac catcgcggcg tttgaaaatg ccgtcagcgc
      601 cgcggaacgc cgggccatcc tcaacaacga aaaggaaacg cccgtatgga tgagcgatct
      661 cgtcggcatc gtaccctcta ttacgggtaa gatcgagctg gtgtatgaag gcgaacagga
      721 aggtccctac caggtggcgt tcaatctgct ggagaaagca ttccgctcgc aattcgtcaa
      781 ttatttcccc aaccccgacc agctaaagaa aaggaaatcc gacagccgcg aagacaatcc
      841 cttccgtccg atcacccact ggttcgacaa agggaaccat ctcaaccttt tcttcaatac
      901 acccgatcac gagaagatac aacagctgta taaagtggac ggtctgcacg cgctggtgaa
      961 aaaatacttc aagcacgcca acgaaagaga ggcggcgtta ttgatggaat tcgtcctgca
     1021 cggtctggcc gcgtattctt tgatcagtaa gaaagtagtc gaaggaaaga tagagtttaa
     1081 ggatctgatc gggtcgatgg tgaacctggg ctcttccggt agtagcttcg gcagcgagga
     1141 cgaggacctg gaaggagatg aattcaactg aggctaatga ttcgccttcg gcgggtgagc
     1201 ctttggctca cagtaaattc acctcgcgct cgaggctgac accgaatttg tcgcgcacgc
     1261 tcgccgcaat ctcttcaccg agccggtaga tatcggcacc gcgggcattc ccataattca
     1321 ccagcaccag cgcctgacgt tcatgcacac ccgcatcgcc cttgcggtag cccttccagc
     1381 cgcactgctc gatcagccaa cccgcagcca gtttcgtgcg ctgtcccagg ggatcggggt
     1441 atccgacgat gcccggaaac ttcgcttgca gctgcgcaaa ctggtcaccc gctactatcg
     1501 gatttttgaa aaaactcccg gcattaccga tctgcgcggg atcgggcagc ttgctccgcc
     1561 ggatggcgat cacggcctgt gaaatggccc ggatactgag ctccttcact cccatcctat
     1621 ccagctcatc ctgtaccgcc ccatagcccg tatgaaagat cggcttcttc cgcaaccgga
     1681 gcgtgacgct caggatcacc gactgatcac gataatgccg tttgaaaaca ctgtcgcgat
     1741 aaccgaactc gcaatccccc agcgtaaaag tacggacatt cctgtccgcc aggctccagg
     1801 cgtcaagttc gaggaataca ttctccaatt ccatcccata cgccccgata ttctgcatcg
     1861 gcgcggctcc gacactcccg ggtatcagcg agaggttctc cacaccggcc caatccctgc
     1921 cgatacagta atcgacgaac ctcgtccagt cctcccctgc cccggcgcgt acatacacat
     1981 gctcttcgtc ctcatccacc agctcgatcc ccttgatggc attctgcagc acgataccgt
     2041 cgatatcccc cgtgaggagg atattactgc cccctcccaa taccacctta cggcggccgc
     2101 cagcccaatc cagcaacccg gccagctcat ctgcggagga aaaggcggaa aaataccttg
     2161 cacgggcatc gatcccgaag gtgttcaaat ttttcaaaga tttattctct attggagtcg
     2221 gcatatatat atttcgcaca tttatacgtt attcaataaa taaggttgta ttttcctacg
     2281 gcgggaccaa ccactcctaa ccagaaccat atgaccaggc atctcatcgc gtgcaccctt
     2341 acgctgattt ccttctccct gtatgcccag gaaaacaatc cgcttatcgt atccggtcaa
     2401 ctgatcgaac aggccgggac cctatacgat tcgggccagt ataagaaggc tatcgacgtc
     2461 tttcggcgga tcgaccgcaa cgataccaac tatatccggg cactgtacgg aatcagctcc
     2521 tgtctatacg aagatagtca gttctccgca tccctcgaat atgccagaaa aggactcgcg
     2581 ctgacggccg atcccgaacg ccggcccgat ctttataacc agatcggcaa taccctcaac
     2641 gagtcggaag ccagcgaaca ggcaataaag gtatacgatt cggctatcaa caaatatcct
     2701 gcctattcca tcctctatct caacaagggt tccgcgctga tcaagctcgg tcgctatccg
     2761 gaggccgaag ccgtcttcaa acaggcgctg ctgaaggacc cctattccta tagctcgcat
     2821 tataagctgg gctattgcgc gatcaaccag ggtaagctgg tcccggcctt tctcagcttc
     2881 atgggttatc ttctcctcaa ccccgaaggc agatacaaat ccagctgcat ccgatggatg
     2941 gacgccatag ccaagaacat ggacacgatc caaaccttcc tcgtccacag gaaagacgat
     3001 gctgccggga actaccagct gctggaacag atattgcaat cgaagatcgc gctggaccgg
     3061 aactacaaga cgatgatcca gctggacgat cccatctccc gccagataca ggtcgtcttc
     3121 gagaaaatga attacgagcc atccgatagc gatttctgga tgcaatacta tatccctttt
     3181 ttcaaatccg ttttttccaa ccgccagttc gaacccttca tcaaccgcct tttcctcgga
     3241 gtagacatcc ccgtcatcca ggaataccac cggcggaaca aaaaagaggt acaggcgctt
     3301 ctcgacgcta cggtcactta tttcaaccat atccgctcga cccgcgagct caactatgcg
     3361 caacgcgaca cggcgtccgc gtcctggttc ttctccaatg gcgagctgtc ttcacacggc
     3421 cggtatctcg tcaaagagga caggcttctc ggcaactgga acttcttcta taacccgggt
     3481 aatatccggt ctacgggcac ctacaatgac aacggcaaaa aagaaggctg gttcaattcc
     3541 tactttttcg acgggaagcc aagaggaaaa gaattctatg tcaacggcga acaggaaggt
     3601 gaagagacct tttatttcaa cagcgggacg atgtcgaccc attcctggta caaaaaggga
     3661 cagctcgaag gcgaagcggt ctcctacttt tgggtaggca ccccgcatac cattacccat
     3721 tatcatgaag gaaaagagga cggcgtaaaa ctcacctatc gtaataacgg cgatacagcc
     3781 cttctcgaac actatacctc cggaactctc aatggcgagg tcaggagttg ggccgccgac
     3841 cgcacggtcg aggtgatcgc gtcctatcag aacggcgagc tggagggaga gtacaggaaa
     3901 tattatccca acggtcagct atacatggat ggcaactaca aggccgggaa acaggacgga
     3961 gaatggaaat cctggtacaa caacggacag ctgaagacgg tcgatcattt cctcaacgat
     4021 aaatccgaag gcgagtacag ggaataccac gacaacggca ccctgcgcta tacggccaac
     4081 cagaagaacg gcaaattcgt cggcgagatc aggtatttcg acgacgacgg aaaggtttac
     4141 agcgtccaga attattcgaa cggtcgcctg caaaaagcga agtatttcga caagacgggc
     4201 gcacagatcg gagagtcgga gagcggcggc tcggggatgg agctggtgga tttcctcccc
     4261 gacgggtcca ggcgcgtcca atacacctct gacgacaagg gaaatatcaa cggcacggag
//
LOCUS       DASYSV010000456         7339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_348393, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000456 DASYSV010000000
VERSION     DASYSV010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348393"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2510)
                     /locus_tag="VGQ51_13985"
     CDS             complement(<1..2510)
                     /locus_tag="VGQ51_13985"
                     /inference="COORDINATES: protein motif:HMM:NF037694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEV9037739.1"
                     /translation="MPNRTGLTAFLLLATLSILSCNRSAVSLDYTNAKDEVPALGNLT
                     FRFDQSLVSDSLVDQWDSTEYVDFEPRIPGRFRWEHPDELVFSPAQPLAPATNYKATI
                     KNAVLQHSKFGRITGNTDLAFHTPDLRVDNVIATWVLQDVNSTSAIPQVELQFNYPVN
                     PNSLKERLSILVGDQPVTYALQTLSASDRVSLRLLNLKMEDKTLEARLAIEKGLLPEG
                     GTNSLAARIELNTAIPSPYVLTIGDVNTEHDGTTGSIRVLTSQQIVPAGLTDAISLSP
                     AVKFTAQVTETGFMISSDNFDQSKSYTLTLGKGIHGRIGGVLHEEYTTNITFGQLEPA
                     ISFVNDKGVYLSGKGAKNIEVKIINVPRIKVVVSKIYENNLLAAPGSGYRPRETTRNS
                     EVRTVSGSEGEGEDEPAENSSSDDLTIGDVIYEQEMDTRLLPRSSTSNINRILNLNIT
                     DKLPEFKGIYHVMIRSTKNYWVRDSRFVSLSDIGMIAKEGKDKILVFTNSIKTATPMT
                     GVTVQAYGANNQLLGAGNTNSDGVAEIAFTRKEFAGFRPAMLIARSADDFNYLPFGTT
                     RINTSRFEVGGKHTNPSGLDAFIYPERDIYRPGEKVNFSVILRDGHWKSPGELPVVLK
                     FLLPNGKELRTFRKTSNAQGSLEGSVDIAGTAITGSYSLEVYTSNDILLATQPFRIEE
                     FMPDRIKVTAQLNKTLLETGDAARLDIHAVNFFGPPAANRNYECEIQVHQLNFRPKNY
                     PDYNFYLSGQSSTFDKNVRQGKTDDQGNAAEKYEVPDIFKNIGQLQASFFTTVFDETG
                     RPVSRRTAIDIYTQPVFFGVAEDGYLFYALNQPINFPL"
     gene            2696..5086
                     /gene="pbpC"
                     /locus_tag="VGQ51_13990"
     CDS             2696..5086
                     /gene="pbpC"
                     /locus_tag="VGQ51_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEV9037740.1"
                     /translation="MKPGFVKRFSNKKMLIWGKRVFAGAAGLFLLFLILNWVFPLPDK
                     IEYSTIVTDNKGEVIHAFLTHDQQWRMKTDLSEISPLLRKTIIEKEDKYFYYHPGVNP
                     LAMLRALGRNILTLHRTSGASTITMQVARELEPKRRTYWNKLVEVFRAFQLEWKYSKS
                     EILQLYLNKVPYGGNIQGVKSASILYFGKNPDHLSLAEITALSVIPNRPSTLVMGKHN
                     DWIVAERNKWLHRWAKEKVFTPKEIEDALAEPLTARRRAVAQLVPQLALKLKRSGGRD
                     IVHTTIDLNMQLKIEKLVKDYSRTLTLKNIRNAAVIVIDNKTHRVVSYVGSADFHDST
                     DGGQVNGAAAVRQPGSTLKPLLYGLCIDAGLLTPKMTIMDVPVNYDGYVPENYDRTFN
                     GYVTMEYALGHSLNIPAVRSLKALGPDVLIRKLSMCGFRQVDKDRRKLGLSMILGGCG
                     ATLEELTGMYSLFANGGRWVRPVYTTDDSSTAPMAFRNAYRTPDAPDSAGGRRLLSPA
                     ATYMISDILSKVNRPDFPVNWQTTVHLPKVAWKTGTSYGRRDAWSIGYNQKYTVGIWV
                     GNFSGVGIPELSGANIATPLLFTVFNTIDYDDDGPWFRQPEDCDIRKVCTETGLPPGD
                     LCDHVVTDLFIPLVSPSKKCDNLQEVFVSADEKISYCRVCLPEAGYKKKIYKIVPPEM
                     KDYFELNRIAYVPIPPHNPMCEKMFHDGGPVVVSPTNGSEYLINRKDPEPVQLRCKVG
                     NDVGKVYWYINDQFFKASDAGTKQFFVPTEGPVKISCTDDKGRNRNIWIRVRYVDM"
     gene            complement(5104..5646)
                     /locus_tag="VGQ51_13995"
     CDS             complement(5104..5646)
                     /locus_tag="VGQ51_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037741.1"
                     /translation="MNLRFLPFLLFLHLAARAQKTPTGPDLPSPEAFVNAIAPTLVDS
                     GLDHYYLVAGTDSCRFLKYNYDEWVKYHLVEPVPFQELNDLAEKVYLSRYPYFWKQAH
                     LHKAICITSHQADSILNAQKNFVYSFSLPQITDDGQYAVIDLNLVCGDRCGKGVTCIF
                     RLTARGDWKLVGENTNWEYH"
     gene            complement(5681..6421)
                     /locus_tag="VGQ51_14000"
     CDS             complement(5681..6421)
                     /locus_tag="VGQ51_14000"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEV9037742.1"
                     /translation="MRKVSQYALILLSCTLLLLSCDDYSGKRVHGNGNVRTEDRSVGN
                     FKNVDVSGAATVLVTQGEKCSIRIEADDNLMQYIEVQQTGDKITIRERPGFHILPANR
                     DRIKVYVTAPVYNDIEASGACDIIGQNKISNSENLSVHVSGAGDIRMELEAPRLDAEV
                     SGSGSIFLKGQTKDVSIDLTGAGDAHCYDLQAENTKVAVTGAGSAEVYASVKLDAEVS
                     GVGSVSYKGHASEVNQQVSGAGSVRKAD"
     gene            6510..>7339
                     /locus_tag="VGQ51_14005"
     CDS             6510..>7339
                     /locus_tag="VGQ51_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV9037743.1"
                     /translation="MTEKILILDFGSQYTQLIARAVREANVYCEITPFSKKIDFDPSL
                     KGIILSGSPFSVNDKGAPEVDIAALHAQLPVLGVCYGAQLTAKQFGGRVANSNKREYG
                     RALLHKQKDGAILQDVSELSQVWMSHADTILDLPAQFEVLANTENIPYAAFRMSDGLP
                     AEGSANGGVGGEGGANGGTHPLYCLQFHPEVYHSIEGKKIIRNFLVNVCGCKQDWTPA
                     HFISDTVAELKRRIGDRQVIMALSGGVDSTVAATLIHKAIGSRLHGIFVDNGVLRKDE
                     "
BASE COUNT         1621 a         1784 c         2260 g         1674 t
ORIGIN      
        1 atcagcggga agttgatggg ctggttcaag gcataaaaca ggtagccgtc ttccgcgacc
       61 ccgaaaaata ccggttgggt atagatgtcg atggccgtcc tgcggctcac cggccgtccc
      121 gtctcgtcaa aaacggttgt aaagaaactc gcctgcagct gaccgatatt tttgaagata
      181 tcgggtacct catatttttc cgcggcattc ccctggtcgt cggtttttcc ctggcggaca
      241 ttcttatcga aggtcgagct ctgaccggac aggtagaaat tataatcggg atagttcttc
      301 ggacggaagt tcagctggtg aacctggatc tcgcattcat agttgcggtt ggcggcgggc
      361 ggaccgaaaa agttgacggc atggatatcc agcctggccg catcgccggt ttccaacaag
      421 gttttattca gctgcgcggt caccttgata cgatcgggca tgaactcctc gatccggaat
      481 ggttgcgtcg ccagcaggat gtcattggag gtgtagactt cgagtgaata gctaccggtg
      541 atggcggtgc cggcaatatc gacacttcct tcgagcgagc cctgtgcgtt ggaggtcttg
      601 cggaaggtgc ggagctcctt gccattgggc aacaggaatt tgagcacgac cggtaattcg
      661 ccgggggact tccaatggcc gtcgcggagg atcaccgaga aatttacctt ttcacccggc
      721 ctatagatat cgcgttcggg atagataaag gcatcgagac cggaagggtt ggtatgtttt
      781 ccgcctactt cgaaacgcga ggtattgatg cgggtggtcc cgaagggcag gtagttgaaa
      841 tcgtccgccg accgggcgat gagcattgcg ggccggaagc cggcaaattc ttttcgtgta
      901 aacgcgatct cggcgacgcc gtcggaattg gtattcccgg cgcccagcag ctggttgttg
      961 gcgccatagg cctggaccgt gacgcccgtc atcggagtag ccgttttgat ggagttggtg
     1021 aagacaagta ttttgtcttt cccctctttt gcgatcatcc cgatatcgga cagcgagaca
     1081 aaccggctgt cacggaccca ataattcttc gtactgcgga tcatgacatg gtagatgcct
     1141 ttgaattccg gcagcttatc ggtgatattg agattgagga tccggttgat gttgctggta
     1201 ctgctgcgtg gaagcaagcg ggtatccatc tcctgctcgt agatgacatc gccgatggta
     1261 agatcgtcgg aggagctgtt ctctgccggc tcatcctccc cttccccttc ggatccgctg
     1321 acggtccgca cctccgaatt acgggtggtc tcccgggggc gatagccgct tcccggcgcc
     1381 gcgagcaggt tgttctcata gatcttggaa acgacgacct tgatgcgcgg cacgttgatg
     1441 atctttacct cgatattttt ggcgcctttc ccggagagat agactccttt atcgttgacg
     1501 aagctgatcg cgggctccag ctgaccgaag gtgatattgg tggtatattc ttcgtggagg
     1561 acgccgccga tgcggccatg gatgcccttg ccaagcgtga gcgtataact cttggactga
     1621 tcgaaattgt cactgctgat cataaagcct gtttccgtca cctgcgcggt gaatttgacg
     1681 gccgggctga gcgagatcgc gtccgtgagg cccgcgggga cgatctgctg gctggtcaaa
     1741 acgcggatgg agccggtggt cccgtcgtgt tcggtgttga catcgccgat cgtcaggaca
     1801 tagggcgagg gaatggccgt attgagctcg atccgggccg ccaggctatt ggtaccgcct
     1861 tcgggcagaa ggcctttctc aatggcgagc ctcgcttcga gcgttttgtc ttccattttc
     1921 agattgagca gccgcaacga cacgcggtcc gaggcggaca gcgtctggag ggcataggta
     1981 accggctggt cacccacgag gatgctcagc cgttccttta gtgaattggg gttcaccgga
     2041 tagttgaact ggagctctac ctgcgggatg gcggaggtgc tgtttacgtc ctgcaatacc
     2101 caggtggcaa tgacgttatc cacgcggagg tccggggtat gaaaggcgag atcggtattg
     2161 cccgtgatgc gaccgaattt gctgtgctgc aggacggcgt ttttgatcgt cgccttgtag
     2221 ttggtggcag gcgcgagagg ctgggccggc gagaagacga gctcgtcagg atgttcccat
     2281 cggaaccggc ccggtatccg gggttcgaaa tcgacgtatt cggtggaatc ccattgatcg
     2341 acgagcgagt cggagacaag ggattggtca aaccggaagg tcaggttacc cagtgccggt
     2401 acttcatctt tggcgttggt atagtcgagg ctgacggcgc tgcggttaca ggaaaggatg
     2461 gacagggttg caagcaacaa aaaggcggtt aggcctgtac gattaggcat aagaggtcag
     2521 gttgaaggct ttttataata cgatgttaat atacaaaaag gacaatcaaa atcaagcggc
     2581 gggtttttgc acgctaacga ataaagaagg aaattagtgt gagcgctaag gaggaatgga
     2641 ggggggcata cagtggcggg acggcgcccg ctgatttagg acttgaaatt acgatatgaa
     2701 accggggttt gtcaagaggt tcagtaataa gaaaatgtta atatggggaa aacgggtttt
     2761 cgccggggcg gcggggttat tcctgttgtt cctgatcctg aattgggtgt tccccctgcc
     2821 agacaagatc gaatattcga cgatcgtgac ggataataag ggcgaggtga tccatgcctt
     2881 cctgacacac gatcagcaat ggcggatgaa gacggaccta tcggagatat ctccgctctt
     2941 acggaaaacc atcatcgaga aagaggacaa atatttctat tatcaccccg gggtgaatcc
     3001 cctggcaatg ctgcgcgccc tggggcggaa tatcctgacc cttcatcgta cgtccggcgc
     3061 gagtacgatc accatgcagg tggcgcgtga gctcgagccc aagcgacgca cctattggaa
     3121 caaactggtg gaggtcttcc gtgccttcca actggaatgg aaatacagta agagtgagat
     3181 cctccagctg tacctgaaca aggtccctta cggcggaaat atccaggggg tgaagtcggc
     3241 atcgatcctt tatttcggca agaacccgga ccatttgtcg ctggcggaga tcacggccct
     3301 gtcggtgatc cctaatcgtc cgtcgaccct cgtcatgggg aagcacaacg attggatcgt
     3361 ggcggagagg aataagtggc ttcaccgatg ggccaaagaa aaggtcttta cgccaaagga
     3421 gatcgaggat gcgctggcgg agccgctcac ggcaagacgt agggcggtgg cgcaattggt
     3481 gccgcagctg gcgctgaagc tgaaacgcag cggcggccgg gatatcgtcc atacgacgat
     3541 cgatctgaat atgcagctga agatagagaa gctggtgaag gactattcgc gcacgctcac
     3601 gctgaaaaat attcgcaatg cggcggtgat cgtcatcgat aacaagaccc accgggtggt
     3661 cagctatgtg ggcagtgccg atttccatga ttccacagac gggggacagg tgaatggggc
     3721 ggcggcggtc cggcagccgg gcagcacgct gaaaccgttg ttatatgggt tgtgcatcga
     3781 tgcgggactg ctgacaccga agatgacgat catggacgtg cccgtaaact atgacggcta
     3841 tgtgccggag aattatgacc ggactttcaa cggatatgtc accatggaat atgcgctcgg
     3901 ccattcgctg aacatccccg cggtaaggag tctgaaagcg ctgggacccg atgtgctgat
     3961 caggaagttg tcgatgtgcg ggttcagaca ggtggacaaa gacaggagga aactgggatt
     4021 gtcgatgatc ctggggggat gcggtgcaac gctggaagag ctgacgggga tgtattcgtt
     4081 gtttgccaat gggggaagat gggtccggcc ggtgtatacg acggatgaca gcagtaccgc
     4141 gccgatggcg tttcgaaatg cgtaccggac gccggacgcc ccggattcag ccggcggaag
     4201 acggctcctg agcccggcgg caacgtatat gatcagcgat atcctgtcga aggtgaaccg
     4261 tccggatttc ccggtaaatt ggcagacgac ggtacatttg ccaaaagtcg cctggaagac
     4321 gggcacgtcc tatgggaggc gggatgcctg gagcatcggg tataaccaaa agtatacggt
     4381 gggtatctgg gtggggaatt tttcgggggt cggcattccg gagctcagcg gcgcgaacat
     4441 agcgacgccg ctcttattca ccgttttcaa tacaatcgat tatgacgatg acggaccctg
     4501 gtttcgacag cctgaggact gcgatatccg caaggtatgc acggaaacgg gtctgccccc
     4561 gggtgatctt tgcgatcatg tcgtgacgga tcttttcatt ccgctggtgt cgccatcgaa
     4621 gaaatgcgac aatctgcagg aggtattcgt gtcggcggat gagaagatat cgtattgccg
     4681 ggtgtgtttg ccggaggccg ggtataagaa gaagatatac aagatagttc ccccggaaat
     4741 gaaggactac tttgagctca acaggatcgc ttatgtgccg atccctccgc ataacccgat
     4801 gtgcgagaaa atgttccatg acggagggcc ggtggtggtc tcgccgacca atggcagcga
     4861 atatctcatc aaccgaaagg accccgagcc cgtgcaattg cggtgtaagg taggaaacga
     4921 tgtggggaag gtgtactggt atatcaatga ccagtttttc aaagcgtcgg atgcgggaac
     4981 aaaacagttc tttgttccga cggaggggcc ggtcaagata tcctgtacgg atgacaaggg
     5041 caggaaccgg aatatctgga taagggtgcg gtatgtggat atgtgagggc ggcgaagggg
     5101 cgtttagtga tattcccaat tggtattttc gccgaccagt ttccagtcgc cgcgggcggt
     5161 cagccggaag atgcaggtga cgcctttccc gcagcggtcg ccgcagacca ggttgagatc
     5221 tatgaccgcg tattgtccat cgtcggtgat ttggggtagc gagaaggaat agacgaaatt
     5281 tttttgggcg ttgaggatcg aatcggcctg gtggcttgtg atgcaaatgg ctttatgcag
     5341 gtgggcctgt ttccagaaat atggatagcg ggagagataa actttttcgg cgagatcgtt
     5401 cagctcctgg aaaggcaccg gttcgacgag gtggtatttg acccactcgt catagttgta
     5461 tttcaggaaa cggcaggagt ctgttccggc tacgaggtaa taatgatcga gcccgctgtc
     5521 tacgagggtc ggggctatag cgtttacgaa tgcttcgggg cttggcaggt ccgggccggt
     5581 gggcgtcttt tgcgcccggg cagccagatg aagaaataaa agaaagggca ggaaccggag
     5641 gttcatgccc caatgtcggc aaaagaacgt taacacggtc ttaatctgcc ttgcggacac
     5701 ttcccgcgcc gctcacttgc tggttgactt cgctggcatg acctttataa ctgacgcttc
     5761 ccactccgct gacctcggca tccagtttta cgctggcata aacctcggcc gagccggccc
     5821 cggtgactgc caccttcgta ttctctgcct gcagatcgta acaatgggcg tctccagcgc
     5881 cggtgagatc gatgctaacg tccttggttt gtcctttcag aaagatggag ccgctgccgg
     5941 agacttcggc atcgagacgg ggcgcttcga gctccatacg gatatctcct gccccgctga
     6001 catggacgga aagattttcg ctgttggata tcttgttctg accgatgata tcgcaggcgc
     6061 cggaggcttc gatgtcatta taaacgggag ccgtcacata tactttgata cgatccctgt
     6121 tagcgggaag gatatggaaa ccgggccgtt cgcggatagt gatcttatcc cccgtctgct
     6181 gcacctctat gtattgcatc aggttgtcat cggcttcgat ccggatggaa catttttcgc
     6241 cctgggtaac cagcaccgtc gccgcgccgc tgacatcgac gttcttgaaa ttgccgacac
     6301 tgcggtcttc ggtcctgaca ttgccgttgc cgtggacgcg tttgcccgag tagtcgtcgc
     6361 aacttaagag caaaagggtg caggacagga ggataagggc gtattgggat acttttctca
     6421 tgatgttggt tttaggttat acacaaaaat aaaggaccca ccgccatcgg ggcacccatt
     6481 gggggatttt ttttaccttc gcgtgatcta tgacggaaaa gatcctaatt ctggacttcg
     6541 gctctcagta tacccaattg atcgccaggg ctgtacgtga agctaatgtg tactgcgaga
     6601 tcactccttt ttccaaaaaa atcgatttcg acccgtcgct gaaaggcatc atcctgtcgg
     6661 gttcgccatt ttcggtgaac gacaagggcg ccccggaggt ggatattgcg gcactgcacg
     6721 cacagttgcc ggtgctgggg gtctgttacg gagcccagct gacggccaaa caattcggtg
     6781 gtcgcgtagc caactctaac aagcgggaat atggacgggc cttgctccac aaacaaaagg
     6841 acggcgccat actacaggat gtcagtgagc tttcgcaggt gtggatgagc catgcagata
     6901 cgatactcga cctgcccgcg caattcgagg tgctggccaa tacggagaat atcccttatg
     6961 cggccttccg gatgtcggac ggactccctg cagaggggag tgccaatgga ggcgtggggg
     7021 gcgagggggg cgccaacggg ggcacgcatc cgttatactg tcttcaattc catccggaag
     7081 tctatcactc tatcgagggc aaaaagatca ttcggaattt ccttgtgaac gtttgtggat
     7141 gcaagcagga ctggacgccg gcccatttta tcagcgatac cgtagcggag ctgaaacgcc
     7201 ggatcggcga ccggcaggtg atcatggcac tcagcggcgg cgtggattcc actgtggccg
     7261 ccacactgat ccataaagct atcggttcgc ggctccatgg catcttcgtc gacaacggtg
     7321 ttttgcgtaa ggacgagtt
//
LOCUS       DASYSV010000457        13440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1118725, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000457 DASYSV010000000
VERSION     DASYSV010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13440
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118725"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGQ51_14010"
     CDS             complement(<1..192)
                     /locus_tag="VGQ51_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037744.1"
                     /translation="MIQKLVVPGALVILLCSCGASKKLKSANAQIEQLQANNAALQNN
                     LDALKKQVSGLSQDNSALSN"
     gene            complement(226..528)
                     /locus_tag="VGQ51_14015"
     CDS             complement(226..528)
                     /locus_tag="VGQ51_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV9037745.1"
                     /translation="MASQRRKFSEAEKLNILESAERLGVTAVLREHRLSYSVFARWKG
                     QYRKNGSEPSIQTMGARTRSELRQTLEENVRLKRIIADQVIELERKEEELRKYRGR"
     gene            complement(659..2974)
                     /locus_tag="VGQ51_14020"
     CDS             complement(659..2974)
                     /locus_tag="VGQ51_14020"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEV9037746.1"
                     /translation="MIRYCLLLLFISATFSVVAQSTSADEQRRWETEASAVTIARDNW
                     GIPHIYAKTDAEVVFGLLYAECETDFARVEKNYLEQLGRQAEAYGENYLYTDVMMRLI
                     YDSAQAVADYERSPAWMHRLLDAFADGVNYYLYKHPEAKPLVLKRFKPWYALMFTDGS
                     VSATSTGGARLDEIKNFYGGHEGLAMGHPVHSMRAVSDVEKMVLADRGIGASDGSDGS
                     VVKGKGQEQWAPEAGNGGDEDERGSNGFALSGFRTASQATELYINPHVPFYFRLEVQL
                     VSEEGLNAYGAVTWGQFFVYQGFNAHCGWMHTSSDADVADLYAEKVKRMDNGWVYEYE
                     SQTRPVKEKPFVVYYKKDSGLVAYPVKGYYTHHGPVMCSREGRWLSLKEYNRSLDALL
                     EAWLITKANTFDEYKKAMDLRANTTNNTVYADDQGNIAYWHGDFMPRRDSSYNWSLPV
                     DGTTAATEWKGVHTLDEIVHVYNPATGWIENCNSTPYTSSGNSSPQRSQYPAYMAPDG
                     ENFRALNAQRMLSGANEFTLDSLISKGYDHYLTAFDVLLPALFSAYGSANDSVRRVLQ
                     EPIGILQKWDRRSGARSVATTLAVEWATRMAGFSRRPRTAEEASHQVDNLQAELEQTT
                     PVQRVGELLAVITELRQRYGNWKIEWGELCRYQRLTGKIVETYDDRKPSLAVGLVSSA
                     FGQLPSFVSRVMPNTKKRYGVSGNSFIAAVEFGKKVTAKTVVTGGESSDPKSPHFTDQ
                     AEMYINGRFKDVLFYKEDVEKHVEREYHPGE"
     gene            3000..3938
                     /locus_tag="VGQ51_14025"
     CDS             3000..3938
                     /locus_tag="VGQ51_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEV9037747.1"
                     /translation="MPGSSIPSPASSIPSPASPPGITILPVHKIQSGQLIETTDPVAV
                     EQPLEIRLQYGPSAARIEQNISLTMRTPGHDTELALGFLFSEGILPGPTPIIRTRSDP
                     DSILICLHESFFPDLSRTQRSSYTNSSCGVCGKLSIDALRRPQPAAAGHPSPSQTAQS
                     FPHTAAGHPSPTAQSFPPISADLLFQLPQRLRASQDIFDSTGGLHAAALFDHSGQIIV
                     LREDIGRHNALDKLIGYCFLRNSLPETNSILILSGRACFELIQKAATAGITIIAAVGP
                     PSSLAVQAAEESGITLVGFLRDRRCNIYSGAHRIRS"
     gene            3950..6463
                     /locus_tag="VGQ51_14030"
     CDS             3950..6463
                     /locus_tag="VGQ51_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEV9037748.1"
                     /translation="MNNEIPSDQPPQDENIPSPESPGTLQNIPSSESPGTLQNIPPSG
                     NPGTLQNIPSPESPGTLQNIPSPENPDALHHLHTGQIAPKAVGIPAILSSFRHVIGEA
                     GLTRGLKALARVNQKGGYDCPSCAWPDPDDERSGIAEYCENGAKAIAEEATTKKLTPE
                     FFAENAVEDLALLSDRDIGSKGRISQPMHLPPGATHYQPISWDEAFKHISDILNSLPS
                     PDQAIFYTSGRTSNEAAFLYQLFARRYGTNNLPDCSNMCHESSGAALSEALGLGKGSV
                     RLDDLYIAEVIIIMGQNPGTNAPRMLTALQKAKRNGATIISVNPLPETGLMAFSNPQQ
                     LPGILGFATQLTDLYLPVRLNGDMALLQAIELLLLLEEQRAPGTVFDLEFIRDRTAGY
                     GEFIEYLQQTSLTDLSEACGIHTADIGKAANILKDKKKIIICWAMGLTQHTNAVDTIR
                     EIANLLLLKGSIGKPGAGTCPVRGHSNVQGDRTMGIWEQPKQAFLDAIEKRFGFRPPQ
                     HHGLDVVNAIRAMHDGKASVFFAMGGNFLSATPDTNYTAEALRRCRLTVHVSTKLHRS
                     HLVHGQEAFILPCLARSDRDFTGGTEQFVSCENSMGVVQSSKGVLTPVSDQLLSEPAI
                     VCRLAKATFEARTPNATFGARAPEANIDWDNYQKNYDTIRNDIENTIPGFTQYNLRVR
                     ESGGFYLPNPPREGRFTTPDQKAHFNVAKPARIDLTNRQLMMMTVRSHDQFNTTIYGL
                     DDRYRGVFNERRVIFLNETDIRDRDLTPNDIVDIHNDHGGIRRTAHRFRVVPYPIPVQ
                     CAATYFPEANVLVPIDTVAAKSNTPVSKLVIITLEKVSP"
     gene            6662..7306
                     /locus_tag="VGQ51_14035"
     CDS             6662..7306
                     /locus_tag="VGQ51_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEV9037749.1"
                     /translation="MLLTDELKESTRTPHQATEKKMVATLKQLRSKEEYIQLLYWLYG
                     FYSPMEDLIGRHLTGNDLAGSMGVPGSDGIGSLSVGDRDDLPYFVGERRSDALLRDIM
                     DSGLPLHHHEDCDHLPVIDSFARALGALYVLEGSALGGRIIAGMIDKQIGLRDSLSFF
                     NGEGAATDKRWQSFKDYLDQPCFNAQRQDILAAAQDTFITFKNWIDKNELQPQL"
     gene            7284..8798
                     /locus_tag="VGQ51_14040"
     CDS             7284..8798
                     /locus_tag="VGQ51_14040"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV9037750.1"
                     /translation="MSSNPNYDSELCGKVPIHLINTVQPYGVLLVISRRSLEIVQASE
                     NVEPLFRMTAPDLIRRSLQDFLDNAAFGFLQRIASGDNSDRIPQIWPIHGEKYLALLH
                     VKESHILAEVDVRPYREEEQSSFVSIYQEIKYSMALIESATTTSAICAIAARELRRIS
                     GFDKVMIYRFDRDWNGTVIAEECEKDMESYLGFTFPASDIPKQARALYLHNPYRYIPD
                     RNYQPVKIYPVINPVTQSFTDLSDCNVRGAASVHLEYLANMGVTASMSTRILNNDSLW
                     GLIACHHRTARHLSFQLCSVFELLSDFISSRMNSLYNKEKQEANIRIRDIYARLLEQT
                     YDSRDLYRGLLEGDATLLQLFDAHGAVMSHGGRLDTKGILPDKENLDEMLLWLNTRRL
                     TRTLHTDHLAGQFDQATQYPDIASGLLAIPYHPEEEKYLLLFRPEEKRIIDWGGDPGG
                     RIVFEKDGLNYHPRHSFKQWREIVSGTSQPWKEEQLTVAENLKSFLYEYTSKQI"
     gene            8773..12942
                     /locus_tag="VGQ51_14045"
     CDS             8773..12942
                     /locus_tag="VGQ51_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEV9037751.1"
                     /translation="MSTQANKFNHYVVAIGASAGGLEAIHEFFDSMPEAPDISFIIVQ
                     HLSPDYKSLLVELVARHTDMKVFEATENQKIERKCVYVIPNTKFITIRKHQLQLVEKK
                     NGKTPNNAVDVFLYSLAKEKKEKAIAVILSGTGTDGTKGTEAIKKAGGLVIVQDPAKS
                     RFDGMPNSVIAAGNADYILPAGQIFREIMQHISPPPAFLYGNEKQDDQFLKTVFDLIR
                     KESGVEFHYYKTPTILRRISKRMIQGSFVDPESYIDYLQDHNEEIKQLGQDFLIGVTR
                     FFRDPEAFDLIREKAVPAIIENKEEDDTIKIWVTACSTGEEAYAIAMVVDEVLEATAK
                     NLTVKIFASDIDPEAIQFASAGEYPLSIEKDVDSRLLQKYFTRTAKGLAIIPRIRKQI
                     VFAKHNIIKDPPFIKNDLISCRNMLIYINPAMQQRIFSLLLFAAGKDGFLFLGPSENS
                     SLIKKDVREISARWKIYQKTAESKPTWYTQQNNPEGFTTRMKDAKQLQLSKDTRSQSL
                     LWEDFKQVLTQDLAVVILYIDAGFDIRETLGPYEKFLALPRRQLHLNLLRMVPSPISI
                     LLSAEIRRAERTGEKIHLKNIRYQKDDTMVATEIYIRPGGRSTGAAPGAPFTMLVLRE
                     LSSEVHSVNGDGRTSNGINGHITHEEEYVRALESELNETKNHLQLAIEDLEATNEELQ
                     STNEELLSANEELQSSNEELQSLNEELITLNTEHQLKIKELLEVNDDLNNYFRSTDIA
                     QVFLDGKLRIRKFNPASSRMINFIDSDMGRPLTHISNNIRSENLLGDVQTVLRTGRPL
                     EKEVQLVSGKNLLMRIMPYLTHEGKNDGAIIAFIDITAITDLNNIIRGVFNSSPSAIF
                     AFQSKRNSKDGIDDFIVLSANYAANAMLRRSNDDLKGLSLKKDLPALATADLLQKYLD
                     VAGGDDSHRGIFRSKEESKWYEFTIVKMMDGFVATFTNITERRLAEDRLRANYMELIT
                     ARETLKKLNLELEDKVIERTRALAISEERFRLVSRVTNDALWDWDFVNNTIWWGDTFY
                     KQFGYETPGTAPTDRKGWLEMIHPDDREATSGSIYLAINSHQSQWSRDYRFRREDGRY
                     AHVLDRAYILHDENGTPYRMLGSMLDITDLKKAEQEIARAKEDLERKVAQRTTQLRQL
                     NDTLETSNQDLQQFASIASHDLQEPLRKIHLFSMAIQDKFGGVLPEEVKIYFEKIIRS
                     TERMRSLIIDILNFSRLSADVPSFRRTDFNTMFAEVLEDFEITIREKQACVSAGQLPE
                     LDVNPGQFRQVLHNLLSNALKFTRPEIPPVIRLESSRIAKKAFDSPPDPDGCFHRFSL
                     TDNGIGFEEKFADNVFKLFQRLHPKDRFEGTGIGLAVTKKIIERHNGLITAYSSENNG
                     SRFEWILPVTR"
     gene            12966..>13440
                     /locus_tag="VGQ51_14050"
     CDS             12966..>13440
                     /locus_tag="VGQ51_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9037752.1"
                     /translation="MLKYKKIVLVDDDTDDRQIFEEILSQIHPDAAVVCAENGLELVS
                     LLDKLPDDQLPEMIILDQNMPQMTGKESLIYLKATPRFRDIPVIVYSTYQIRDFYNEC
                     LELGAQDVVPKPDTLQAYRNMIQQILITPPPSPSISPESLIQSTPAEVFIPSHPPE"
BASE COUNT         3280 a         4390 c         2958 g         2812 t
ORIGIN      
        1 attgctcaac gcgctattgt cctggcttag acccgacacc tgttttttca gggcgtcaag
       61 gttattctgc aaggctgcat tattggcctg aagctgctcg atctgcgcgt tggcgctttt
      121 cagcttcttg gatgcgccgc acgaacataa caagatgact agggcgccag gtacgacgag
      181 tttttgtatc ataataattg gtgtttattg tatttaagcc ctccctcacc ggcccctgta
      241 tttccgcagc tcttcctctt tgcgttcgag ctcgatcacc tggtccgcga ttatcctctt
      301 caaccggacg ttctcttcca acgtctgccg gagttcggac cgcgttcggg cgcccatagt
      361 ctggatggag ggctcgcttc cattcttccg gtactgccct ttccatctcg cgaatacact
      421 ataggatagc cgatgttccc gcaacacggc cgtcacccca agccgttccg cgctctcgag
      481 gatgttgagc ttttccgctt cgctgaattt tcttctttga ctggccatca gggtatattt
      541 ccccatcaag acacattaaa agcctgtctt ccgccatttt gtcttaaaaa aaaggcccga
      601 gctatcgctc aggccaaatt ctaactcgtg tctcgcgctc tggcaagctc gcttcttgtt
      661 attctccggg atgatactcc ctctccacat gcttctccac atcctcctta taaaaaagca
      721 catccttgaa ccttccgttg atatacattt ccgcctgatc cgtaaaatgc ggcgacttcg
      781 gatcgctgct ctcccctccg gtcaccaccg tcttcgcggt caccttcttt ccgaactcca
      841 ccgccgcgat aaaactattc cccgataccc cataccgctt tttggtattc ggcatgaccc
      901 ggctgacaaa tgaaggcagc tgcccaaatg ccgaagacac cagccctacc gcaagactcg
      961 gcttcctgtc atcataggtt tcgacgatct tacccgtcaa acgctgatat cggcacagtt
     1021 cgccccactc gatcttccag ttgccataac gctgccgcag ctcggtgatg acagccagga
     1081 gctcacccac ccgctgtaca ggagtagtct gttccagttc ggcctgaaga ttgtccacct
     1141 ggtgcgaagc ttcctccgct gtccggggcc gacgtgaaaa accggccatt ctcgtcgccc
     1201 actccactgc cagggtcgtg gccaccgaac gggcccccga ccggcgatcc catttctgca
     1261 atatcccgat gggctcctgc aacaccctgc gcacactgtc attagccgat ccgtaggcgc
     1321 tgaataaggc cggcaacaat acatcgaatg ccgtaagata gtggtcatat cctttgctga
     1381 tcagactgtc cagcgtgaac tcattggctc cgctcaacat ccgttgcgca ttcagtgccc
     1441 tgaaattctc tccgtccggc gccatatagg ccgggtactg actacgttga ggactgctgt
     1501 tccccgacga ggtataagga gtggaattgc agttttcgat ccatcccgtc gcggggttat
     1561 agacatgcac gatctcatcc agcgtatgaa cgcctttcca ttcggtggcg gccgtagttc
     1621 catccaccgg caacgaccag ttgtacgagc tgtcccggcg aggcatgaag tcaccatgcc
     1681 aataggcaat attcccctga tcatccgcat atacggtatt gttggtcgta ttggccctga
     1741 gatccatggc ttttttgtac tcatcgaagg tattggcctt cgtgatcagc catgcctcga
     1801 gcaaggcgtc aagactgcgg ttatattcct tgagactcag ccaccttcct tcacggctgc
     1861 acatcaccgg tccatgatgc gtataataac cctttaccgg gtatgccacc agcccactgt
     1921 cttttttgta gtatacgacg aacggtttct ctttcaccgg tctcgtttga ctttcatatt
     1981 cgtagaccca tccgttgtcc attcgtttca ctttctccgc atacagatcg gctacatccg
     2041 catcgctcga ggtatgcatc cagccgcagt gcgcgttgaa cccctggtaa acgaagaatt
     2101 gcccccaggt cacggcccca tacgcattga gcccttcttc actcaccaac tgcacttcca
     2161 gacgaaaata aaatggtaca tgcgggttaa tatacagctc ggtcgcctgc gaggccgttc
     2221 ggaaacccga aagcgcaaaa ccattgctgc ccctctcatc ctcatccccc ccattccccg
     2281 cctccggcgc ccactgctcc tgacccttgc ccttcaccac actaccatcc gacccgtcgg
     2341 acgccccgat ccccctgtcc gccagcacca tcttttccac atccgacaca gccctcatgg
     2401 aatgcactgg atgtcccatc gccaatccct catgcccacc ataaaaattt ttgatctcat
     2461 ccaatctggc cccacccgta ctcgtcgccg atacactgcc gtcggtgaac atcagcgcat
     2521 accacggttt gaaacgcttc aataccaggg gcttcgcctc cggatgttta taaaggtaat
     2581 agttgacccc gtccgcaaat gcgtccagca accggtgcat ccacgcggga ctacgctcat
     2641 aatctgccac cgcctgagcg ctgtcataga tcagccgcat catgacgtcc gtataaagat
     2701 agttttcgcc atatgcttcc gcctgccggc ccagttgctc caggtagttc ttctccaccc
     2761 gcgcgaagtc cgtctcgcac tcggcataca aaagcccaaa gactacttcc gcatcggtct
     2821 ttgcataaat atgcgggata ccccaattgt cccttgcaat ggtcacggct gaagcctccg
     2881 tttcccaccg tcgctgctcg tcagcggatg tcgactgtgc cacaacagaa aaagtcgccg
     2941 aaataaacaa caatagcagg caatacctaa tcatccccca atttaatgta aatttggata
     3001 tgcccggttc ctccattcca tccccagctt cctctattcc atcgcccgct tcaccccccg
     3061 ggatcaccat cctgcccgta cataagatac agtccggcca actcatcgag accactgacc
     3121 ccgtcgccgt cgaacaaccc ctcgagatcc gcttgcaata cggaccctct gcagcccgca
     3181 tcgaacagaa tatttccctg accatgcgca ctcccggcca tgataccgag ctggccctcg
     3241 gttttctctt ctccgaaggc atcctccccg gtccgacacc gatcatccgg acacgatccg
     3301 atcccgattc catccttatc tgtcttcacg aatccttctt tcccgatctc agccggaccc
     3361 aacgcagctc ctacaccaat tccagctgcg gcgtctgcgg caaattatcc atcgatgcgc
     3421 tccgcagacc acaaccagcc gccgccggac atccgtcccc ttcacagact gcccagtcct
     3481 tcccgcacac cgccgccgga catccgtccc cgacagccca gtccttcccg cctatctccg
     3541 ccgatctcct gttccaactc ccccaacgcc tccgcgcgag ccaggatatc ttcgattcca
     3601 cgggcggcct ccacgccgcc gctctcttcg atcactccgg ccagatcatc gtcctccgcg
     3661 aagacatcgg acgtcacaat gccctcgaca aactcatcgg ctactgcttt ttgcgcaata
     3721 gtctcccgga aacaaattcc atcctcattc ttagcggacg cgcctgcttc gagctcatcc
     3781 agaaggccgc cacagccggc atcactataa tagccgcagt aggacctccc tccagcctcg
     3841 cagtacaggc cgcggaagaa tccggcatca ctttggtagg cttcctgcgg gacagaagat
     3901 gtaacatata ctcgggcgcc caccgcatcc gatcatgaat attaatctta tgaacaacga
     3961 aatacctagc gatcagcctc ctcaggatga aaacattcct tcccccgaga gcccaggcac
     4021 ccttcaaaac attccttcct ccgagagccc aggcaccctt caaaacattc ctccctccgg
     4081 gaatccgggc acccttcaaa acattccttc ccccgagagc ccgggcaccc ttcaaaacat
     4141 tccttccccc gaaaatccgg acgccctcca ccatcttcat accggccaaa tcgctcccaa
     4201 agcggtcggc atccccgcca tcctgtccag cttccgccat gtcatcggtg aagccggtct
     4261 tacccgcggt ctcaaagcat tggcccgcgt caaccaaaaa ggaggatatg actgccccag
     4321 ctgcgcatgg ccggaccccg acgacgaacg ttcaggtata gccgaatact gtgagaatgg
     4381 cgccaaagcc attgccgaag aagcgacgac caaaaagctc accccggaat tctttgccga
     4441 aaatgcagtc gaagacctgg cactgctctc cgaccgggac atcggcagca aaggccgtat
     4501 ctcccaaccg atgcacctgc cgccaggtgc tacccattac caacccatct cctgggacga
     4561 agccttcaaa cacatatccg acatcctcaa ttcccttccc tcacctgacc aggccatttt
     4621 ctatacttct ggccgtacca gcaacgaagc ggcatttctc tatcaactat tcgcccgccg
     4681 gtacggtacc aataacctgc ccgattgttc caacatgtgt cacgaatcca gcggcgccgc
     4741 actcagcgag gccctcggtc tcggtaaagg ctcggtccgt ctcgacgatc tctatatcgc
     4801 ggaagtgatc atcatcatgg ggcaaaaccc cggcaccaac gctccgcgga tgctcaccgc
     4861 tctgcaaaaa gccaaacgca atggagccac catcatctcc gtcaatccgt taccggaaac
     4921 aggtctgatg gccttcagca atccgcaaca actacccggc atcctcggct tcgcgaccca
     4981 gctcaccgat ctctatctac cggtcaggct caacggcgat atggcgctcc tccaggcaat
     5041 cgagctcctg ctactactcg aagaacaaag ggcgccgggc accgtcttcg atctggagtt
     5101 catccgtgac cggaccgccg gttacggtga gttcatcgaa tacctgcaac agacctccct
     5161 gaccgacctc tcggaggcat gcggtatcca caccgccgac atcggcaagg ccgcaaacat
     5221 cctgaaagat aaaaagaaga tcatcatatg ctgggcgatg ggcctcaccc aacacactaa
     5281 tgcggtcgac accatcaggg agatcgccaa cctgctgctg ctcaaaggta gcatcgggaa
     5341 acccggcgcc ggaacatgcc cggtacgcgg ccacagcaac gttcagggcg acaggaccat
     5401 gggcatttgg gaacagccga aacaagcctt cctggatgcg atcgaaaaac gattcggttt
     5461 tcgcccaccg caacaccacg gtctggatgt cgtaaatgcc atccgggcga tgcacgacgg
     5521 caaagcctca gtatttttcg ccatgggcgg taatttcctt tcagctaccc ccgataccaa
     5581 ctatacggca gaagctcttc gacgctgccg gctcaccgtc catgtctcaa caaagctgca
     5641 tcggagccat ctcgtgcacg gacaggaggc cttcatactt ccctgcctgg cgcgtagtga
     5701 ccgggacttc accggcggta ccgaacagtt cgtctcctgc gagaattcca tgggcgtcgt
     5761 ccagtcttcc aaaggcgtgc tcacgcccgt ttcagaccag ctgctcagcg aaccagccat
     5821 cgtctgccgc ctcgccaaag ccacgttcga agcgcgcacg cccaacgcca cgttcggagc
     5881 gcgcgccccc gaagccaaca tcgactggga taattaccaa aaaaattacg acaccatccg
     5941 caacgatatc gaaaacacca tccccggttt cacccaatac aatcttcgtg tgcgcgaaag
     6001 cggtggcttc tacctgccga acccgccccg cgaaggccgc ttcaccaccc ccgaccaaaa
     6061 agcgcatttc aacgttgcca aaccggcccg gatcgatctc acaaaccgcc agctcatgat
     6121 gatgaccgtc cgcagccacg accagttcaa cacaactatc tatggcctcg acgaccggta
     6181 ccgcggcgtc ttcaacgaac gacgggtcat cttcctcaac gagaccgata tccgcgaccg
     6241 cgacctgacc ccgaatgata ttgtagacat ccacaacgac cacggcggca tccgccggac
     6301 ggcccatcgc ttcagagtgg tcccctatcc cattccggta caatgcgccg cgacctattt
     6361 tcccgaggcc aatgtgctcg tcccgatcga tactgtggcc gctaaaagca atactcccgt
     6421 atccaaactg gtcatcatta ccctggaaaa agtttccccc tgatccctct tacggatgtc
     6481 tctcacccat ccgcccccaa aaaatggccg cgtttttgtt ccattccaca ctgggtttca
     6541 tattccttta taatatgggt actgctcccg gcctccggcg tagttcaacg gggaagtttt
     6601 tccccaacca ttgtacaatc gaatgcgcat cccgccacaa acatcatcaa ttcattcagg
     6661 tatgttattg actgacgaac taaaggagtc cacgcgtacg cctcaccagg caacggaaaa
     6721 aaagatggtc gcgacgctga aacaactccg ctcgaaagag gaatatatac agctactcta
     6781 ctggctctat ggattctata gcccaatgga agatctgatc ggccgccatc ttacggggaa
     6841 cgacctggca ggctccatgg gcgtccccgg gtctgacggt atcggctctc tgagcgtcgg
     6901 agatcgtgac gatcttccct attttgtcgg agagagacgg tcggatgctc ttctccggga
     6961 tatcatggat tccggtctgc cgcttcacca ccatgaagac tgtgaccacc tgccggttat
     7021 cgactccttc gcccgtgccc tcggcgcgct ctacgttttg gaaggaagcg ccctcggcgg
     7081 tcgcatcata gcaggcatga tcgacaaaca gatcggcctc cgcgatagcc tgtctttttt
     7141 caatggcgaa ggagccgcga ccgacaaacg ctggcagtcg ttcaaagact acctcgatca
     7201 accctgtttc aacgcccagc gacaagatat cctggcggcg gctcaggata cgttcataac
     7261 cttcaaaaac tggatcgata aaaatgagct ccaaccccaa ttatgattcg gaactctgcg
     7321 gcaaagtccc cattcatctg atcaacaccg tacaaccata cggcgtcctg ctggtcatct
     7381 cccgcaggtc actggagatc gttcaggcaa gcgaaaacgt agaacccctt ttccggatga
     7441 ctgcacccga cctgatccgg cgatccctgc aggatttcct cgacaacgcc gctttcggct
     7501 ttcttcaacg gatcgcctcc ggcgacaact ccgacaggat accccagatc tggcccatcc
     7561 acggcgaaaa ataccttgcc ctgctgcatg taaaggaaag ccatatcctc gccgaagtcg
     7621 atgtacgacc ctacagagaa gaagaacaaa gctccttcgt cagcatctat caggaaataa
     7681 aatactccat ggcgctgatc gaaagcgcta ccaccacttc ggccatatgt gccatcgccg
     7741 cccgcgagct ccgacgcatc tccggcttcg ataaggtcat gatctatcgc ttcgaccgcg
     7801 attggaacgg tacggtcatc gccgaagaat gcgaaaagga tatggaatcc tatctgggat
     7861 tcaccttccc cgcatcggat attccaaaac aggcccgcgc gctctacctt cacaatccat
     7921 accggtatat ccccgatcgt aattatcagc ccgtcaagat ataccccgtc atcaatcctg
     7981 ttacccaatc ctttaccgat ctttccgact gcaatgtccg gggcgcggcc tctgtccacc
     8041 tggagtacct ggccaatatg ggcgtcactg cttccatgtc tacccggatc ctcaacaacg
     8101 attcactctg gggcctcatc gcctgtcatc acagaactgc cagacatctt agcttccagc
     8161 tctgttcggt gttcgagttg ctttcggatt tcatatcctc ccgaatgaat tccctctaca
     8221 acaaggaaaa acaagaggcg aatattcgta taagagacat ttatgcccgt ctcctcgaac
     8281 aaacatacga cagccgggac ctttaccgtg gccttctgga aggtgatgcg accctcctcc
     8341 aactgttcga tgcccacggc gcagtcatga gccatggtgg ccggctcgat acgaaaggta
     8401 tcctccccga taaggaaaat ctcgatgaga tgctgctgtg gctcaataca cgccgcctta
     8461 cccggacact ccataccgat catctcgccg gacaattcga ccaggcaacc caatatccgg
     8521 acatcgcgag cggtctgctc gccatcccct accaccccga agaagaaaaa tacctcctcc
     8581 tgttccgccc tgaagaaaaa aggatcatcg attggggcgg cgaccccgga ggccgcatcg
     8641 ttttcgaaaa agacggactg aactatcacc cccgtcactc attcaaacaa tggcgggaga
     8701 tcgtcagtgg cacctcccag ccatggaagg aagaacaact cacggtagcc gaaaacctga
     8761 agagcttttt atatgagtac acaagcaaac aaatttaatc actacgttgt agcgatcggc
     8821 gcatcggcgg gcggcctcga agccatccac gaattcttcg acagcatgcc cgaagccccc
     8881 gatatttcgt tcattattgt ccagcacctc tctccggatt acaaaagcct cctggtagag
     8941 ctcgtagccc gtcacaccga catgaaggta tttgaggcca ccgaaaatca gaagatcgaa
     9001 cgaaaatgcg tatacgtcat ccccaacacc aagttcatca ccatccggaa acaccagctc
     9061 cagctggtgg aaaagaagaa cggtaagacc cctaataatg cggtagacgt cttcctttat
     9121 tccctcgcta aagaaaaaaa ggaaaaagcg atagcagtga tcctttccgg caccggaacc
     9181 gacggcacga aaggtaccga agcgatcaaa aaagcaggtg gtctggtcat cgtccaggac
     9241 cctgccaaat cccggttcga tggtatgccc aacagcgtca ttgcggcggg caatgccgac
     9301 tacatcctgc ccgccggaca gatcttccgg gagatcatgc aacatatctc gccacccccc
     9361 gctttcctct acggcaacga gaaacaggat gaccagttcc tgaaaacggt attcgatctc
     9421 atacggaagg aatccggcgt cgagttccat tactacaaaa cccccaccat cctgcgacgt
     9481 atttccaaac gcatgatcca gggcagtttc gtcgaccccg aatcatacat cgactacctt
     9541 caggatcaca atgaagagat caaacagctg ggacaggact tcctcatcgg cgtcacccgc
     9601 ttcttccgtg accccgaagc cttcgatctc attcgcgaaa aggcggtccc cgccatcata
     9661 gaaaataaag aggaggatga tacgatcaag atctgggtca ccgcctgcag tacgggagaa
     9721 gaagcctacg ccatcgcaat ggtcgtcgat gaggtgctgg aagcaacggc aaaaaacctc
     9781 accgtaaaga tcttcgcttc ggacatcgat ccggaagcca tacagttcgc ttcggcgggg
     9841 gaatatccgc tttccattga aaaagatgtg gactcccgtc tccttcaaaa atatttcacc
     9901 cgcaccgcaa aaggcctggc catcattccg cgaatccgga aacagatcgt cttcgccaaa
     9961 cacaatatca tcaaggaccc tccttttatc aagaacgacc tcatttcctg ccggaatatg
    10021 ctcatctata tcaatccggc tatgcagcaa cgcatcttct cactgttgct gttcgcggcc
    10081 ggcaaagacg gcttcctctt cctcggccct tccgaaaact cctcgctcat caaaaaagac
    10141 gtccgcgaga tcagcgcccg ctggaagatc tatcaaaaga ccgccgaatc caaacccacc
    10201 tggtacaccc agcagaataa tccggaaggc tttaccaccc ggatgaaaga cgccaaacaa
    10261 ttacagttgt caaaggacac gcggtcgcaa tccctccttt gggaagattt caaacaggta
    10321 ttgacgcagg acctcgccgt cgtgatcctc tatatcgacg caggcttcga tatacgcgaa
    10381 acacttgggc cttatgaaaa attcctggcc ctcccgcgcc ggcagctgca cctcaatctt
    10441 ctccggatgg tgccctctcc tatatccatc ctgctcagcg ccgagatccg acgggccgaa
    10501 agaacaggcg agaagataca tctgaaaaat atccgctacc agaaggacga tacgatggtg
    10561 gccacggaga tctatatccg gcccggagga agaagcacag gtgctgcacc gggcgctccc
    10621 tttaccatgc tcgtcctccg cgagctctcc tccgaagtcc attcagtcaa cggcgacggc
    10681 agaacctcca acggcatcaa cggccatatc acccatgaag aagaatacgt ccgcgccctg
    10741 gaatccgaac tcaacgagac caaaaatcac ctgcagctcg ccatcgaaga cctcgaggcc
    10801 accaacgagg aacttcagtc tacaaacgaa gagctcctct cggccaacga agaactccaa
    10861 agctccaacg aagagctcca gtcgctgaat gaagagctca tcaccctcaa tacggaacac
    10921 cagctcaaga tcaaagagct gctcgaggtg aacgacgatc tcaacaacta tttccggagc
    10981 accgacatcg cccaggtctt cctcgacggg aagctgcgca tccgcaagtt caaccctgca
    11041 tcttcccgga tgatcaattt catcgattcc gacatgggaa gacccctgac ccatatctct
    11101 aataatatcc ggtcggagaa cctgctcgga gacgtacaaa ccgttctgcg caccggccgg
    11161 ccactcgaaa aagaggttca gctggtcagc ggcaagaacc tcctcatgcg catcatgccc
    11221 tatctcacgc acgaagggaa gaatgacggg gctatcatag ctttcataga tatcaccgcg
    11281 ataaccgatc tcaataacat catccgcggg gtcttcaaca gcagccccag cgccatattc
    11341 gccttccagt cgaagaggaa tagcaaagac ggcatcgacg atttcatcgt gctttccgcc
    11401 aactacgccg ccaacgccat gctccgccgg tcgaacgacg acctgaaagg cctctcgctg
    11461 aaaaaagacc tgcccgcgct ggccacagcc gatctgctcc agaaatatct cgacgtggcc
    11521 ggcggcgacg acagccaccg cggcatcttc cgcagcaagg aggaatccaa atggtacgaa
    11581 ttcaccatcg tgaagatgat ggacggcttc gtcgccactt tcaccaacat caccgaaaga
    11641 cggctggcag aagatcgcct gcgggccaat tatatggaac tcatcaccgc ccgcgaaacc
    11701 ctcaaaaagc tcaacctcga gctcgaagac aaggtcatcg aacggaccag ggcacttgcc
    11761 atcagcgaag aaagattccg gcttgtctcg cgcgtcacca acgacgccct ctgggactgg
    11821 gacttcgtca acaataccat atggtggggt gacacttttt ataaacaatt cggctacgaa
    11881 acgccgggca ccgcgcccac cgatcgcaaa ggatggctgg agatgatcca ccccgatgac
    11941 cgggaagcca cctccggaag catttattta gccatcaatt cccatcagtc tcaatggtcg
    12001 agggactacc gattccgccg ggaagacgga cgctacgccc acgtcctcga ccgcgcctat
    12061 atcctccatg atgaaaatgg tacaccttac cgaatgctcg gttccatgct cgatatcacc
    12121 gatctcaaaa aggccgagca ggaaatagcc cgggccaaag aggatctgga acgcaaggtg
    12181 gcccaacgaa cgacccagtt gcggcagctg aacgacaccc tcgagaccag caaccaggat
    12241 ctccagcagt tcgcctccat cgcttcgcac gatctgcagg agccactccg caagatccat
    12301 ctcttctcca tggcaataca ggacaaattc ggaggagtcc ttcccgaaga agtaaaaatt
    12361 tattttgaga agatcatccg aagcaccgaa cgcatgcgat cgctgatcat cgacattctc
    12421 aatttctctc ggctttccgc cgacgtgccc agtttcagac gcacggattt caacaccatg
    12481 ttcgccgaag tcctcgaaga tttcgagatc accatccgcg aaaaacaggc ttgcgtaagc
    12541 gccggccagc tacccgaact cgacgtcaat ccgggtcagt tcagacaggt gctgcataac
    12601 ctcctcagca acgccctcaa gttcacccgc cctgaaatac cacccgtcat ccgtctcgaa
    12661 agctcacgca tcgccaaaaa ggcattcgac tcaccgccgg accccgatgg ctgcttccac
    12721 cggttcagcc tcaccgacaa tggcatcgga tttgaagaaa aatttgccga caacgtcttc
    12781 aaactctttc aacgccttca ccccaaagac cgcttcgaag gcacaggtat tggcctggcc
    12841 gtgaccaaaa agatcatcga gcgccataat ggcctcatca ccgcttacag cagcgagaac
    12901 aatggctccc gcttcgagtg gatattgccc gtaacccgtt gaaaatgata aaaaccgatg
    12961 ttgttatgct gaaatacaag aaaatagtgc tggtggacga tgacacggac gatcgtcaga
    13021 tatttgaaga gatactatct caaatacacc cggacgcagc cgtggtgtgc gcagagaacg
    13081 gcctggaatt ggtgtcgctt ttggacaagc ttcccgatga ccaattaccg gaaatgatca
    13141 tccttgacca gaatatgccc caaatgacag ggaaggaaag cctcatctat cttaaggcta
    13201 ctccccgctt ccgcgacatt cccgtcatag tctattctac ttatcagata agggattttt
    13261 acaacgaatg ccttgagctc ggcgcacagg atgtcgtccc caaaccggac accctccagg
    13321 cataccgcaa tatgatccag cagatcctca tcaccccacc cccttctcca tccatttcac
    13381 ctgaatcgct cattcaatcc acccctgccg aagtcttcat tccatcccat ccccccgaag
//
LOCUS       DASYSV010000458         8995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2140050, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000458 DASYSV010000000
VERSION     DASYSV010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140050"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VGQ51_14055"
     CDS             <1..231
                     /locus_tag="VGQ51_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-specific adenosine deaminase"
                     /protein_id="HEV9037753.1"
                     /translation="EPCPMCLGALYWARPRRVVYAATRDEAAAAGFDDAFIYREIDLE
                     PAERKIPFVHEPMEGASEIFGSWREKGDRRLY"
     gene            complement(308..799)
                     /locus_tag="VGQ51_14060"
     CDS             complement(308..799)
                     /locus_tag="VGQ51_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037754.1"
                     /translation="MTRSINQLSHTLLKGSKIAAFIAALLLTVGISSSFATPNNDRTD
                     AVTASFRKDFKKAELLATESGKNFTKLTFKMNDVVLFAFYSGDGNLLAVVRNIKLSQL
                     PIQLMLKVKQDYSDYWISDLFELDADGSTNYYITLENANSTITLRSADTTTWETYSKK
                     TKE"
     gene            complement(1251..1901)
                     /locus_tag="VGQ51_14065"
     CDS             complement(1251..1901)
                     /locus_tag="VGQ51_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV9037755.1"
                     /translation="MKVYFISGIGADYRFFTHVRLPEGYEASYIHWIPPQRNETLAAY
                     ALRLADQIDSSQPFILVGLSLGGIMSVEIAQKIPPVCTILISSVPSAAHLPKLYKLAG
                     KLRLGRLIPATLLKLAALIRHSLLMRPAENRRLMRQVILAGDDSFIRWALNAVLDWNN
                     RTIPQPLYHIHGTRDEVFPISLTTPSHIVRKGDHMFVVSRPETVNQFLREVLPPIH"
     gene            complement(2092..2865)
                     /locus_tag="VGQ51_14070"
     CDS             complement(2092..2865)
                     /locus_tag="VGQ51_14070"
                     /inference="COORDINATES: protein motif:HMM:NF011765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEV9037756.1"
                     /translation="MKPLNQHLAKALSLLLLLSACHNATDDQPDRSPKAVMPAATAAP
                     MRTSVLTATDQQALSPDKVIADLKNGNRNYQSNHPTPMNDTAMMRATAKTQYPEAFIL
                     SCMDSRVPVEKIFDKGIGDLFVGRVAGNIIDEDVLGSMEYGCKLAGARLIVVLGHESC
                     GAVKAAIEGEQLGNVTGLLAHIQPAIQLSHSTQGAHDNGNPMFVTAVIRQNVRNSIRD
                     IKAESPVLKELAEKGEIRIVGAYYSLHTGEVTFFDDDHR"
     gene            complement(2965..3041)
                     /locus_tag="VGQ51_14075"
     tRNA            complement(2965..3041)
                     /locus_tag="VGQ51_14075"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3005..3007),aa:Ala,seq:ggc)
     gene            3141..3314
                     /locus_tag="VGQ51_14080"
     CDS             3141..3314
                     /locus_tag="VGQ51_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6728 family protein"
                     /protein_id="HEV9037757.1"
                     /translation="MGVWRQIQEYFYIRKRDPDAPNTQWMRYMHGINRISIFMFLIGL
                     IVLIVRLLFFKHH"
     gene            complement(3413..4702)
                     /locus_tag="VGQ51_14085"
     CDS             complement(3413..4702)
                     /locus_tag="VGQ51_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEV9037758.1"
                     /translation="MKYYIIAGEASGDLHGSNLIRQLKEQDPQADVRCWGGDKMQASG
                     GHVVKHYKELAFMGFAEVVMNLRTIFRNMDWCKQDILKYRPDVLILIDYPGFNMRIAK
                     WAHHLSSPSAEALHPNRPFPIVYYISPQVWAWKEGRVRQIRANVDKMLVILPFEQAFY
                     KKWDYPVEYVGHPLVEVIDEARTQPSPPSLAKSPSAPASPSVPGSSSAPGSSSAPASS
                     SAPASPSAPAPPPFDKPVIALLPGSRKQEILKKLPIMLQVSRDFPDYQFVVAQAPGLE
                     PDFYEHLLAGYSNVASVTNQTYALLLMARAACVTSGTATLETALFGVPEVVCYKGSPI
                     SYQIARRLIRVKYISLVNLIMDRLVVHELIQDDLTPQNLRRELDDILHNPTRLAQLEK
                     DYRELKELLSQGGHASANAARSILQFLQQTPASPERA"
     gene            4818..6113
                     /locus_tag="VGQ51_14090"
     CDS             4818..6113
                     /locus_tag="VGQ51_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV9037759.1"
                     /translation="MPAFNRRSFIDAMGKFSATAFLATLARPAWSRNLDRALLGAEER
                     SPQQLAGDEDFWYYVQQSFTVSPELINLNNGGVSPSPKVVQEAMKRYLDFSNEAPSYY
                     MWRILDQGREPLRKDLARIAGCSPEEIAINRNASEALETVIFGLPLKAGDEVVVCKYD
                     YPNCINAWKQRELREGIRIVWVDLVLPSEDNEYLASQYVKAFTAKTKVVHVTHVINWN
                     GQIIPARKIADAAHQRGIEVLVDGAHSFAHFEFSIPSLGADYFGTSLHKWMAACIGSG
                     MLYVRKDKIGPLYPLFAAGEPKSDNIRKFENLGTRPFFIEQAIGKAIDFYDMIGAARK
                     EQRLFYLKNYWMTAVKDVPRVKISTSQKKGFGCAIGMVSVEGKTASALEEYLFTTYKV
                     HTVAINWENLHGIRVTPNVYTTTIDLDKLIAGIREFANT"
     gene            6110..7426
                     /locus_tag="VGQ51_14095"
     CDS             6110..7426
                     /locus_tag="VGQ51_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV9037760.1"
                     /translation="MKREIGLLQATSINMIDMVGIGPFVTMTIVISVINSNLFLWAWV
                     FGAVTALIDGMIWSELGAAYPLAGGSYNFLKEAYGTKWGPLMSFLFIWQTCLQAPLVV
                     ASGAIGFAQYFGYIVPMGYWGEKAVSGTVVVIIIALLYRNIRAIGKITVFLWAGVIIT
                     ILLIIVAGFTHRTVPYSCFPSANQSLFSWAFMTLVGQASVKTIYSFLGYYNVCHLGGE
                     IKDPGRNIPRSIFISIFCIGALYLAMNISVVGVIPWQQAMHSNFVVSLFMEKIYGHRM
                     AVVVTVLVLWIAFASLFAVVLGYSRVPYAAAADGHFFPVFARLHPTKDFPYVSLLFIG
                     ALGLVFSLRLKLEYAISAILAMRILVQFVAQAIGVVVLRKRKGTTGLPFKMWLYPAPV
                     ILSVIVWLFVLYHTGPVALYGVWLAAIGVVVFYLTRGRWRRVNKMM"
     gene            7437..8897
                     /locus_tag="VGQ51_14100"
     CDS             7437..8897
                     /locus_tag="VGQ51_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV9037761.1"
                     /translation="MKRRRFLRQGLVAGAGMIGGGRLMGAMVPPDFPVVRIPEAKRKF
                     KSLSVEKTIREVRETIGNKELGWLFENCFPNTLDTTVDFSMTDSRPDTYVITGDIDAM
                     WLRDSSAQVWPYLSLCKTDKDLQQLIHGVIHQQTRCILKDPYANAFYKDDTKISEWKA
                     TDITDMKPGIHERKWEIDSLCYPIRLAHGYWQMTGDTSPFDADWLAAMQKVLQTFREQ
                     QRKDGEGPYSFQRKTSWATDGVPMGGYGYPTKKVGLIHSMFRPSDDATIYPFLIPSNI
                     FALNSLRDLRTMLTAMSTGKDLEGRAFSLEEELAGALIEHAIVTHPVYGEVFAYEADG
                     FGNHTFMDDANVPSLLALPYLQSPHYSGDLSVIHPEKRGYAVYQNTRRMVLSESNPFY
                     FKGKAGEGIGGPHAGLNMIWPISIVIRGLTSHDSMEVAKCLKLLQNSHAGKGLMHESF
                     HKDDATKYTRPWFAWANTLFGEFVLKTYREQPALLS"
BASE COUNT         1990 a         1909 c         2917 g         2179 t
ORIGIN      
        1 gagccatgcc cgatgtgtct gggtgccttg tattgggcga ggccgagacg ggtggtctat
       61 gcggcgacca gggatgaggc ggctgctgcg gggtttgatg atgctttcat ctaccgcgag
      121 atagatttgg agcccgcgga acggaagata ccgttcgttc atgagccgat ggaaggtgcg
      181 tcagagatat tcggatcatg gagggaaaag ggggatagga ggttgtatta acccggaggg
      241 aaggaggatc ggtgactata ttaaaaaacc ccgcagatgc ggggcttttc attgaaaggg
      301 aagacgatta ctcttttgtt tttttgctat acgtttccca ggtagtggta tccgcggaac
      361 gaagggtgat cgtgctgttt gcgttttcga gcgtgatgta atagttggtg gaaccatcag
      421 catccagttc gaaaagatcg gagatccagt agtcggaata atcctgtttt actttcagca
      481 tgagctggat gggcagttgg gacaatttga tgttccgtac gactgccagg aggttgccgt
      541 caccggagta gaacgcgaac aggaccacgt cgttcatctt aaaagtgagc ttggtgaagt
      601 tcttgcccga ctcggtggcc aatagttccg cttttttgaa gtctttgcgg aaagaggcgg
      661 tcacggcgtc ggtccgatcg ttgttggggg tggcgaagct gctgctgatg cctacggtca
      721 ggagcagggc ggctataaaa gcggcaattt tactcccctt taaaagggtg tggctgagtt
      781 ggttgattga gcgtgtcata tacttaggtt ttaatgttta ctaattttag cgggaaggac
      841 ctcactgttt gtttgcataa caaagataag gggaagaaca ggtactatac aatacatagt
      901 acagtgaatg gcataatact atgttaagcg gtaaaaaacg cgggatgagc tgcgattgga
      961 tgggctcttt gcttttgagg aatgggtgtt tttgattcat gaatgtagtt tttgcggctt
     1021 gcatggggtc ggctgaggga ccactgcggg cgaagagcgg agtgccagag gggtgccaag
     1081 aaataatctt gttgataatc aatatgttgt gggatttgga tgaaatggga ccgtacggtt
     1141 ttgatttgac ggtgtccggt ttgggatgga cggcggggaa tgttaaaagg agaggcggat
     1201 ggggcagtga gagatgataa tggaaaggcg ggagtacggc acgaagacat ttaatgaata
     1261 ggcggcagga cttcgcggag gaattggttg acagtttcgg ggcggctgac cacgaacata
     1321 tggtcgcctt ttcgaacgat gtggctcggg gttgtcagac taatggggaa gacttcgtcg
     1381 cgggtgccat ggatgtgata aagcggctgg ggaatggtgc gattattcca atccaacacg
     1441 gcgttgaggg cccagcggat gaaactgtcg tcgccggcga ggatcacctg tctcatgagc
     1501 cgcctgtttt ctgcgggtcg catcagcagg gaatgtctga ttaatgcggc gagcttcaaa
     1561 agtgtagcgg gtatgagccg gccaaggcga agtttgcctg ccagtttata gagtttgggc
     1621 agatgcgctg ccgaagggac gctactgata aggatagtac acacgggtgg gatcttttgg
     1681 gcgatctcga cggacatgat gccgccgagc gacaaaccca cgagaatgaa gggttgggaa
     1741 gaatcgatct gatcggcgag gcgcaatgca taggcggcta gtgtttcgtt gcgttgtggc
     1801 gggatccagt gaatataaga agcttcatag ccttcgggaa gacggacgtg ggtgaagaaa
     1861 cggtagtcgg cgccgatgcc cgagatgaaa tagactttca tgacgggaaa ttagggaaat
     1921 taaatttctc ggagagtccg gggaaggggg tgtaaatttg gagatgaaat ttgaagagga
     1981 tgaaaatctc ggaaggaaat agcggtaaga tccaactgta taagattgaa gccacctgaa
     2041 acggcggctt tttttattac gtgtaaatga gggggaggcg ttgtgggaaa gttatcgatg
     2101 gtcgtcgtcg aaaaaggtga cttcgcctgt gtgtagactg taataggcgc cgactatcct
     2161 gatctcgcct ttttctgcca gctctttgag gaccgggctc tcggctttga tatcccggat
     2221 cgagttgcgt acgttctgcc ggatcaccgc ggtaacgaac atcgggttgc cgttgtcgtg
     2281 ggctccctga gtggaatggg atagttgtat ggctggttgg atatgtgcca gcaaccccgt
     2341 aacatttccg agctgttcac cttcgatggc cgccttgacg gcgccgcagg attcatgacc
     2401 caggactacg atgagcctgg cgcctgccag cttgcagcca tattccatgc taccgagcac
     2461 gtcttcgtcg atgatgttgc cggctacgcg accaacgaag agatcgccga tgcctttgtc
     2521 aaatatcttt tcgaccggaa cgcggctatc catgcaggaa aggatgaagg cttcgggata
     2581 ttgcgtcttg gcggttgccc tcatcattgc ggtgtcgttc atgggggtgg ggtggttgga
     2641 ttggtagttg cggttaccat tttttaggtc ggcgatgacc ttgtcgggag acagggcttg
     2701 ttggtcggtg gctgtcagga cggaggtgcg catgggtgcg gcggtggcgg ccggcattac
     2761 ggctttggga gatcgatcgg gctgatcgtc ggtcgcgttg tgacaggcgg agaggagaag
     2821 gaggagcgat aaggctttgg cgaggtgttg gttgagtggt ttcatgacca ctgcaaggtt
     2881 ttgaactatt ggggagtggc aaaataatga ggggggaaag atgccaggat ttgcaaaaaa
     2941 aagcgaagaa aggatttctt cgcgtggtgg agaatatcgg agtcgaaccg acgacctctt
     3001 gcatgccatg caagcgctct agccagctga gctaattccc ctttaatgga taggttttcg
     3061 ggagtgcaaa tatagaggat tttgcataaa ccattaaaaa atcggggtgt tatctatctt
     3121 tagattgtaa ttttatcatt atgggagtct ggagacagat acaagaatat ttttacatca
     3181 ggaaaaggga tcccgatgcg ccgaataccc agtggatgcg gtatatgcat gggatcaacc
     3241 gcatttctat attcatgttc ctgatcgggt tgatcgtcct gatcgtgcgg ttattgtttt
     3301 tcaaacatca ttagggagag gccggaaggg atgggtaatc ggacgcgttc gtgacgaacc
     3361 gggagtgacg gggaatcgga tgctttcgtg acgaaccggg agtgatgggg aatcaagctc
     3421 gttctggaga ggccggggtt tgctgcagga attgaaggat gctgcgtgcg gcgttggcgg
     3481 aggcgtggcc gccctggctg agcaattctt tcagctcgcg gtaatccttt tcgagttggg
     3541 cgaggcgggt tggattgtgg aggatgtcgt cgagctcgcg gcggagattt tggggggtga
     3601 gatcgtcctg gatcaattca tggacgacga gacggtccat gatcagattg acaagggaga
     3661 tgtattttac gcggatcagc cggcgggcga tctgatagga gatggggctt cctttatagc
     3721 agaccacttc gggtacgccg aacaaggcag tctcgagggt ggcggtgccg gaggtgacac
     3781 aggcggccct ggccattaat agcagcgcat aggtctggtt ggttacggaa gcgacattgg
     3841 agtagccggc gagaaggtgt tcatagaaat cgggttctag accgggagcc tgtgcgacga
     3901 cgaattggta gtcgggaaag tcgcggctca cctgcagcat aatggggagt tttttcagga
     3961 tctcctgttt gcggctgccg gggaggagcg cgatgacggg tttgtcgaaa ggggggggtg
     4021 cgggtgcaga tggggaggcg ggtgcagatg aggaggccgg tgcggatgag gagccgggtg
     4081 cggatgagga gccgggtacg gatggggagg cgggtgcgga tggggattta gcgagtgacg
     4141 gcggggaagg ctgcgtgcgg gcttcgtcta tgacctcgac gagcggatga ccgacgtatt
     4201 cgacgggata gtcccatttt ttgtaaaagg cctgctcgaa tggaaggatg acgagcattt
     4261 tgtcgacgtt ggcccggatc tggcggacgc ggccttcttt ccaggcccaa acctggggag
     4321 agatataata gacgatagga aatggccggt tgggatggag cgcttcggcc gatggcgatg
     4381 aaaggtggtg ggcccatttg gcgatgcgca tgttgaagcc ggggtaatcg atcaggatga
     4441 ggacgtcggg ccgatatttt aggatatcct gtttgcacca gtccatgttg cggaagatgg
     4501 tacgaagatt catcactacc tcggcgaatc ccataaaggc aagctcttta tagtgtttga
     4561 cgacgtggcc gccggaagcc tgcatcttgt cgccgcccca gcagcggacg tcggcctgag
     4621 gatcctgttc tttcagttgt cggatgagat tggagccatg gagatcgccg gaggcttcac
     4681 cggcaatgat gtaatacttc atgcgggggt gaaggtacga tttaatagga agcgggaatg
     4741 agaggggcgg gcgagcaatg ggggaagcgg gaagtggggc agaatgctta tattgggggc
     4801 actaaactgc tacacgtatg cctgccttta accgacgttc ctttatcgac gcgatgggaa
     4861 aattttccgc tacggccttt ttggcgacgc tggcgcgtcc ggcgtggagc cgcaatctcg
     4921 acagggcgtt gttgggtgcg gaggagcgat cgccgcaaca gctggcgggg gatgaggatt
     4981 tttggtatta cgtgcagcag tcctttacgg tcagcccgga gctgatcaat ctaaataacg
     5041 ggggggtgtc gccgtcgccg aaggtcgtgc aggaggccat gaagaggtat ctcgattttt
     5101 caaacgaggc gcccagctat tatatgtggc ggatactcga ccaggggagg gagccgttgc
     5161 gtaaggacct cgcgcggatt gcgggctgtt cgcctgaaga gatcgctatc aaccgcaacg
     5221 cctcggaagc gctggagacg gtgatcttcg ggctgcccct gaaggcgggc gatgaggtgg
     5281 tggtttgcaa atacgattat cccaactgta tcaatgcctg gaagcaacgc gagctgcgcg
     5341 aaggcatccg tatcgtatgg gtggatctcg tgctgccgag cgaagacaat gagtacctgg
     5401 cgtcgcaata tgtaaaagca tttaccgcga agaccaaggt tgtgcatgtc acacatgtga
     5461 ttaactggaa cggtcagatc atcccggcgc ggaagatcgc ggatgccgcg catcaacgtg
     5521 gaattgaagt gctggtcgac ggtgcgcata gcttcgctca ttttgaattc agcattcctt
     5581 cactaggtgc cgattatttc gggaccagtc ttcacaaatg gatggcggcc tgtatcggga
     5641 gcggcatgct ctatgtaaga aaggacaaga tcggcccgtt gtatccgctg tttgccgccg
     5701 gcgagccgaa gtccgacaac atccggaaat tcgagaacct tgggacccgg ccgtttttta
     5761 tcgagcaggc catcgggaag gccatcgatt tctatgatat gatcggcgcg gcgcggaagg
     5821 agcagcggct attctatctg aagaactatt ggatgacggc ggtaaaagat gtgccgaggg
     5881 ttaaaatttc aacttcgcaa aagaaaggtt tcggctgcgc tatcggtatg gttagcgtcg
     5941 aaggtaagac ggcatccgcg ctggaggaat atcttttcac cacctacaag gtccataccg
     6001 ttgccatcaa ctgggaaaac ctgcatggga tccgcgtgac accgaatgtg tataccacca
     6061 ccatcgatct ggacaaactg atcgcgggta tcagggaatt tgccaacaca tgaagcggga
     6121 gatcggtctg ctccaggcta cctccatcaa tatgatcgac atggtcggca tcgggccttt
     6181 cgtcacgatg accatcgtca ttagcgttat caacagtaac ctattcctat gggcctgggt
     6241 attcggcgcg gtgaccgcgc tcattgacgg gatgatctgg agcgaactgg gggcggcgta
     6301 tccgctggcg ggaggaagct ataacttcct gaaagaagct tatggcacta aatggggacc
     6361 gttgatgtcc ttcctgttta tctggcaaac ctgtctgcag gccccgttgg tcgtggcatc
     6421 gggtgccatc ggtttcgcac aatatttcgg ttatatcgtg ccgatgggtt attggggaga
     6481 aaaggccgtc tccgggacgg tggtcgtcat cattatcgcg ttgttgtacc gcaatatccg
     6541 cgcgatcggg aagataaccg tattcctgtg ggccggcgtg atcattacca tattactcat
     6601 catcgtcgcc gggttcacgc atcgcacggt cccctacagt tgtttccctt cggcaaatca
     6661 atccctattc agctgggcat tcatgaccct ggtgggtcag gcctccgtta aaacgatcta
     6721 tagtttcctc ggttattaca acgtttgtca cctgggcggc gagatcaagg accccgggcg
     6781 caatatcccc cgcagcatct ttatttcgat cttttgcatc ggcgcgttgt atctcgctat
     6841 gaatatcagt gtggtcggag tcataccgtg gcagcaggcg atgcactcca attttgtcgt
     6901 cagccttttc atggaaaaga tctatggcca ccggatggcg gtggttgtca ccgtgctggt
     6961 gttgtggatc gcctttgcgt ccttgttcgc ggtggttctc ggctattcgc gggtgcccta
     7021 tgcggcggcg gccgacgggc atttctttcc ggtgttcgcg cggctgcatc cgacgaagga
     7081 ttttccctat gtgtcgctgt tgttcatcgg ggcgttggga ctcgtgttca gtctgcggtt
     7141 gaagctcgag tatgcgatca gtgcgatatt ggcgatgcgt atcctggtgc agtttgtcgc
     7201 gcaggcgatc ggggtggtgg tgttgcggaa gaggaagggt acgacgggac tgcctttcaa
     7261 aatgtggcta tacccggctc ccgtgatatt gtccgtgatc gtctggttgt tcgtgttgta
     7321 ccacacgggc ccggtggcgc tgtatggggt gtggctggcc gcgataggag tggtagtgtt
     7381 ctatctgacg cgtggccggt ggcgaagagt gaataaaatg atgtagtttg cgggatatga
     7441 aaagacgaag atttttacgg cagggtttgg tggcgggagc gggaatgatc ggaggtggtc
     7501 gattgatggg ggcgatggtc ccgcctgatt tcccggtggt aaggatacct gaggcgaaaa
     7561 gaaaattcaa aagcctgtcg gtggaaaaga cgatccgcga ggtgcgcgag acgatcggca
     7621 ataaagagct tggctggttg ttcgagaact gtttccccaa tactttagac acaacggtgg
     7681 atttttcgat gacggatagc cggcccgata cctatgtcat cacgggcgac atcgacgcca
     7741 tgtggttgag ggacagcagc gcgcaggtgt ggccgtatct ttcactatgc aagaccgaca
     7801 aagatctgca gcagctgatc cacggggtca tccaccagca gacccgatgt atcctgaagg
     7861 atccgtatgc caatgccttt tataaggacg atacgaagat cagcgaatgg aaggcaacgg
     7921 atatcacgga tatgaaacct ggcatccacg aacggaaatg ggagatcgac agtctttgtt
     7981 atcccatccg gctggcgcat ggatactggc agatgaccgg cgatacttct cctttcgatg
     8041 cagactggct ggccgcgatg cagaaggtgt tacagacctt tcgggaacag cagcgcaaag
     8101 acggggaggg accttatagt tttcagcgga agacgagctg ggctaccgac ggcgtgccga
     8161 tgggcggata tggatatccg acaaagaagg tcgggctcat acattcgatg ttccgtccga
     8221 gcgacgatgc gacgatctat ccgttcctga taccttcgaa tatctttgcg ctcaatagtc
     8281 ttcgcgatct tcggacgatg cttaccgcga tgtcgacggg caaggatctc gagggacggg
     8341 ctttttcgct ggaggaagaa ctggcgggcg cattgatcga gcacgctatt gtcacccatc
     8401 cggtatatgg tgaagtattc gcttatgagg cggatgggtt cggcaatcat acgtttatgg
     8461 atgacgccaa tgtgccgagc ctgctggcat tgccgtatct gcagtcgccg cattattcag
     8521 gtgatctttc ggtgatccac ccggagaagc gtggatatgc cgtgtatcag aatacgaggc
     8581 ggatggtgct gagtgaatcg aatcctttct attttaaagg caaggccggg gaaggcatcg
     8641 gcggtccgca tgcggggttg aatatgatct ggccgatcag tatcgtgata cgagggttga
     8701 ccagccatga ttcgatggag gtggctaagt gtttgaaatt gctgcagaat agtcatgccg
     8761 ggaaggggct catgcatgag tctttccata aagatgacgc gacaaagtat acgcggccat
     8821 ggtttgcatg ggcgaatacg ttgtttggag aatttgttct taagacatat cgcgaacagc
     8881 cggctttgct gagctaggct accgacacga ggtagtaatt cttttttccc ttctggacca
     8941 ggatgtattt gccatgcagg agcaatgagg cgtctacgga tagttttgca tcttc
//
LOCUS       DASYSV010000459         6372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_991467, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000459 DASYSV010000000
VERSION     DASYSV010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991467"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..653)
                     /locus_tag="VGQ51_14105"
     CDS             complement(105..653)
                     /locus_tag="VGQ51_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEV9037762.1"
                     /translation="MATILPVEGKTPCFGKNCFIAPNATIVGDVEMGNDCSIWFNAVV
                     RGDVNSIRIGDKVNIQDGVVIHGTYKKAGVTIGNDVSIGHNAIVHGCTIQERVLIGMG
                     AIVMDNAEVGKNSIIAAGAVVLEGTKIESGTIYGGVPARKIKDIPPDLISGQIERIAN
                     HYLTYAGWFRDCNEAPQHAPQP"
     gene            700..1878
                     /locus_tag="VGQ51_14110"
     CDS             700..1878
                     /locus_tag="VGQ51_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV9037763.1"
                     /translation="MSEDTSRLFLRHVAQTSPNPLGLEIVKAEGCYLYDRSGKTYLDL
                     IGGISVCNTGHRHPQVVQAVKDQADRYLHLLVYGELIQTPQVEYATLLAAHLPPSLES
                     VYFANSGAEATEGAMKLAKRITGRTEIVAFHQSYHGSTQGALSVMGDEYWRNAFRPLL
                     PGIRHVVYNSEEALAAITDRTACVIAETVQAERGVYAPDAGWMEALRRRCDAAGALLI
                     LDEIQTGFGRTGTIWGFERYGIVPDILLLGKALGGGLPMGAFIASRERMWKLTGDPVL
                     GHITTFGGHPLCCAAGLASFRVLLESGVAGDVNQKEALFRSRLVHPSIKAVRSCGLLI
                     AVEFDSWETNKRVIDACIAGGVLTDWFLFAPQCLRIAPPLNISVEQIEEGCRKILEAL
                     "
     gene            1989..2306
                     /locus_tag="VGQ51_14115"
     CDS             1989..2306
                     /locus_tag="VGQ51_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037764.1"
                     /translation="MSQFLEQKELFDGVLLLGEEEIREPIVVFERFFSDYRLHECRYI
                     LWSMVETCLTTENTEFSDPGERANLLLNYRNLERLLEAGTLLLKKHYAALQRVQPAAK
                     EKN"
     gene            complement(2485..3192)
                     /locus_tag="VGQ51_14120"
     CDS             complement(2485..3192)
                     /locus_tag="VGQ51_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEV9037765.1"
                     /translation="MSPFWGEFIGTALIIVLGQGVVCNVVLQKTKGNNSGWIVITWGW
                     AMAVFVGVYCSAAASKAHLNPAVTLAFAVLGKITWQLVPKYLAAQFLGAMTGSLVAWL
                     CFRGHYSQTSDANMKLATFSTMPAIRNYGNNLITETIATFILILGILFIAAPTASLGA
                     LDALPVSLLILAIGLGPGGPTGFAINPARDLGPRIMHAILPIPGKRDSDWGYAWVPVL
                     GPILGALLAALAYHALQ"
     gene            complement(3204..4739)
                     /locus_tag="VGQ51_14125"
     CDS             complement(3204..4739)
                     /locus_tag="VGQ51_14125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEV9037766.1"
                     /translation="MDRNNMIARLAAGDGQPYDICIIGGGATGLGIAVDAASRGYKTV
                     LLEQYDFAKGTSSRATKLVHGGVRYLQQGNVRLVTEALHERGILRRNAPHIVKDLSFI
                     VPNYKWWEGPYYGLGLKVYDWMAGSLGIGKSRLLSREETLQLAPTLDDDGLRGGVLYY
                     DGQFDDARLAINLAQTAAHLEAVVLNYCRVDGFLKKQDHIEGVATTDTISGRSYNIPA
                     RIVINATGVFTDNILRMDDPGSETVISPSQGVHIMLDKEFLPGDAAILVPHTDDGRVL
                     FAVPWYDKIIIGTTDTPVDNIDIEPRALPEEITFILRQISRYLKKDPGYGDIRSVYAG
                     LRPLVKNSGRKTAELSRDHLILVSGSGLITITGGKWTTYRRMAEDTVNTAISRGGLPD
                     KPCVTADLPILGADDKTSVGSLAAAHPQWGEKLHPRLPYILADIVHAARTELCMTVED
                     ALSRRTRSLLLDAQAAIDCAETVAAILAEQLGRDREWQQRQVEDFKQLANGYLVHPSI
                     VNS"
     gene            complement(4721..6226)
                     /gene="glpK"
                     /locus_tag="VGQ51_14130"
     CDS             complement(4721..6226)
                     /gene="glpK"
                     /locus_tag="VGQ51_14130"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV9037767.1"
                     /translation="MPSSYILSLDQGTTSSRAILFDHNGRIQQMAQKEFRQFYPQPGR
                     VEHDPEEIWSSQYGVMAEVVAKAGITTKDIAAIGITNQRETVIVWERSSGKPIHPAIV
                     WQDRRTAEYCDRLKTDGHTPTIRERTGLIIDAYFSASKLKWILDEVPGARASAAKGDL
                     AFGTVDTWLVWKLSNKSLHITDTTNASRTMLFNIHTGAWDDDLLKLFDIPRNILPEVR
                     SSSEIYGRTASIMAPGSPGIPISGIAGDQQAALFGQLCTQPGMVKNTYGTGCFLLMNT
                     GSKAVDSKNQLITTIAWTREGKTEYALEGSIFIGGAVVQWLRDGLHFIRDSAQIEQLA
                     ASVDTTDGVYMVPAFAGLGAPHWNQYARGTLFGMTRGTTQAHISRAALESIAYQTYDV
                     LKAMEADSGIILKELRVDGGATANNLLMQFQSDILNVPVVRPTVTETTALGAAYLAGL
                     GTGYWSSLDDIRKQWAEDRRFTPAMDTTKTQPLLKGWQRAVKAAIAWTETI"
BASE COUNT         1254 a         1552 c         2052 g         1514 t
ORIGIN      
        1 ggcgggcgtt aggagggttt gtgggggggg tcggcgcgga aggggcatgg gggacggcgg
       61 cgggggggca gcgcggaagg gggctggggg atggcgggcc gcggtcaggg ctggggcgcg
      121 tgctgcgggg cttcgttgca atcgcggaac catccggcat aggtgaggta gtggttggcg
      181 atgcgttcga tctgacccga gatgaggtcg ggcgggatat ccttgatctt gcgtgcgggt
      241 acgccgccat agatggtgcc ggattctatt ttcgtgcctt cgaggacgac ggctcccgcg
      301 gcgatgatgg aatttttacc gacttccgca ttgtccatca cgatggcgcc cattccgatg
      361 agcactcttt cctgaatggt gcagccgtga acgatggcat tatgaccgat ggatacatcg
      421 ttaccgatgg tgactccggc tttcttatag gtgccatgga tgacgacgcc atcctgaata
      481 ttcaccttgt cgccgatgcg gatgctgttg acatcgccgc ggacgacggc attgaaccag
      541 atgctgcaat catttcccat ctccacatct cctacgatgg tagcgttcgg cgcgataaaa
      601 caattttttc caaagcatgg ggttttgcct tccaccggta ggatcgttgc catagagagt
      661 aatttgattg tttggtaaat atacgaactt taggtctcca tgagtgaaga tacgagcagg
      721 ctattcctga gacatgttgc gcaaacgtca cccaatcctc ttggtctgga gatcgtaaag
      781 gcggaaggct gttatctcta tgaccgcagt ggtaagacct acctggacct tatcggcggg
      841 atcagtgtct gcaataccgg tcaccgacac ccgcaggtag tacaggcggt gaaggatcag
      901 gcggaccgtt atctgcatct gttggtgtat ggcgagctga tccagacacc tcaggtggaa
      961 tatgccacgc tgctggccgc gcatcttcct ccgtcacttg agtcggtgta tttcgcgaac
     1021 agcggggcgg aggctacgga gggcgcgatg aagctggcca agaggataac gggtcgcaca
     1081 gagatagtcg cctttcatca gtcttatcat ggcagcacgc agggagctct gagcgtcatg
     1141 ggtgatgaat actggcgcaa tgcgtttcgt ccgttattgc ccggtatccg gcatgtggtt
     1201 tataattcgg aggaggcgct ggcggccatt acggacagga cggcttgcgt cattgcagaa
     1261 acggtccagg cggagcgcgg agtgtatgcg cccgatgcgg gttggatgga ggctttgcgg
     1321 cggcgatgcg atgctgccgg cgctttgctg atcctcgatg agatccaaac ggggttcgga
     1381 cggacgggca ccatctgggg tttcgaacgg tatgggatcg taccggacat cctgcttctg
     1441 ggaaaagcgt tgggcggcgg tttgccgatg ggcgcattca ttgccagtcg cgagcggatg
     1501 tggaaattga caggcgatcc ggtcctgggg catattacca ctttcggggg tcatccgctg
     1561 tgttgtgcgg cggggctggc atctttcagg gtattgctgg aatccggtgt ggcaggagac
     1621 gtgaaccaaa aggaagcgct gttccgcagc aggctcgtgc atccctcgat aaaggccgtg
     1681 cggagctgcg ggttgctgat cgcagtggaa ttcgacagct gggagacgaa taagcgtgtc
     1741 atcgatgcct gtattgccgg cggtgtgctc accgactggt ttctgttcgc tccgcagtgt
     1801 cttcgtatcg ccccgccgtt gaatatcagc gttgagcaga tagaggaggg gtgcagaaaa
     1861 attttggaag ctctctgaag gaatttgtaa attgtgttgc ttcctttttt gcggataagt
     1921 gcagcgtccg cttcgaatta agatagctat cccgggcgcg gcccaatctt aacctaaaaa
     1981 atattcatat gtcacaattc ctggaacaga aagaactgtt cgacggggtc ctgttgcttg
     2041 gggaggaaga gatacgggag cctatagtgg tattcgaacg atttttcagc gattaccggt
     2101 tgcatgaatg ccggtatatc ctttggagca tggttgaaac gtgtctgact acggaaaata
     2161 cggagttcag cgatcccggg gagcgggcca atctcctgct caattatcga aatctggaac
     2221 gtttactcga agccggtact ctgctgctga agaagcatta tgcggcctta cagcgggttc
     2281 aaccggctgc caaagaaaaa aattaatccc gtgtgaaaac caattggcag ggtttttcgt
     2341 atatagtaca gaatgagaag aataattttg ctaggttttt gattagtgaa tccccgccgc
     2401 aaggcgggga ttttttttgg gattttttgg atggaggtga agatggcgtc cgcaaccgat
     2461 tgcggtgcga ctcgatttgc ggccctattg cagcgcgtga taagcgagtg cggccaggag
     2521 ggcgccgagg atggggccca gtaccggtac ccaggcgtag ccccagtcgc tatcccgttt
     2581 gccggggatt ggaagtatag cgtgcatgat acggggaccg agatcgcggg cgggattgat
     2641 ggcaaaaccc gtcgggcctc cggggcccag tccgatggcg agtatcagca agctgaccgg
     2701 cagagcgtcg agggctccca ggctggcggt gggtgcggca atgaagagga tacccagaat
     2761 gaggatgaag gtggcgatcg tctcggtgat cagattgttt ccataattgc ggatagccgg
     2821 catggtactg aaggtggcga gcttcatatt ggcatcactg gtctgtgagt aatgtccgcg
     2881 gaagcatagc caggctacga gcgagccggt catagcgccg aggaattgcg cggccagata
     2941 tttcggaacg agctgccagg taatttttcc caatactgcg aaggccagcg tcacggctgg
     3001 gttgagatgc gccttgctgg ctgctgccga gcaatagacg ccgacaaata cggccattgc
     3061 ccaaccccag gtaatgacga tccatccgct gttgttgcct tttgtttttt gcagtactac
     3121 gttgcagacc acgccctggc ccaggacgat gatcagggcg gtgccgataa attctcccca
     3181 gaaaggactc atatgcgtga agtttaagaa ttgacgattg atggatgaac gagatagcca
     3241 tttgcgagtt gtttgaaatc ctctacttgt cgttgctgcc attcgcggtc cctgcctagt
     3301 tgttccgcaa ggatcgccgc tacggtttct gcgcaatcga tggctgcctg tgcgtcgagc
     3361 aggagactgc gggtccgacg gctaagggcg tcttctacgg tcatacatag ctcggttctc
     3421 gcggcatgga cgatgtcggc aaggatatag ggtaagcgcg ggtgcaattt ctctccccac
     3481 tgcgggtgcg ccgccgcaag cgatccgacg gacgtcttgt cgtctgcgcc taaaatgggc
     3541 aggtcggccg tgacgcaggg tttatcgggt aggccaccgc gtgagatggc cgtattgacg
     3601 gtgtcctccg ccatcctgcg gtaggtggtc catttgccgc cggtgatggt gatgagcccc
     3661 gagccggata cgaggatcag gtggtcacgg gagagctcgg ccgtttttct gccgctgttc
     3721 ttcaccagtg gccgcagacc cgcatagacg ctgcggatat ctccgtagcc gggatctttt
     3781 ttgagatagc ggcttatctg ccggaggata aaggtgatct cttccggcag ggcgcgcggt
     3841 tcgatatcga tgttgtccac cggggtatcg gtggtcccta taatgatctt gtcgtaccag
     3901 ggtacggcaa acagtacccg gccgtcatcg gtgtgaggga cgagaatcgc cgcatcgccg
     3961 ggcaggaatt ccttatccag catgatatgt acgccctggc tgggggaaat gacggtctcg
     4021 ctaccggggt cgtccatacg aaggatgtta tcggtgaaca cacccgtggc gttgatgacg
     4081 atgcgcgcgg ggatattgta cgaccggccg ctgatggtat ctgtagtggc cacgccttcg
     4141 atgtggtctt gctttttgag gaaaccatcc acccggcaat agttgagtac aacggcttca
     4201 agatgtgcag cagtctgggc aagattgatc gcgaggcggg cgtcatcgaa ctgaccgtcg
     4261 taatagagga cgccgccgcg gaggccgtcg tcatcgaggg taggggccag ttgaagtgtt
     4321 tcctcgcgcg agagcagccg cgatttgccg atacccaggc tgccggccat ccagtcgtat
     4381 accttcagac ccaggccata ataggggcct tcccaccatt tgtaattcgg gacgatgaaa
     4441 gaaagatctt ttacgatatg gggagcgttg cggcggagga tgccgcgttc gtgaagggct
     4501 tcggtcacca gccttacatt gccctgctgg agatagcgta ctccgccgtg gaccagcttt
     4561 gtagcacgcg atgaggtgcc cttggcaaaa tcgtattgtt cgagcagcac ggttttgtaa
     4621 ccgcgggaag ccgcgtctac ggctattccc agaccggtgg cgccgcctcc gatgatgcag
     4681 atgtcataag gctggccgtc tccggcagcc agccgggcta tcatattgtt tctgtccatg
     4741 cgatagcagc tttgaccgcg cgttgccagc ctttgagcag gggttgtgtt tttgtggtat
     4801 ccattgccgg tgtgaagcgg cggtcttccg cccattgttt tcggatgtca tccagtgatg
     4861 accagtagcc ggtgccgaga ccggccagat atgccgctcc gagggcagtc gtctcggtga
     4921 cggtaggtct tacgacaggg acgttcagga tatcggactg aaactgcatc agcagattgt
     4981 tggcggttgc cccgccgtcc acgcgtagtt ccttcaggat gatgccgctg tcggcttcca
     5041 tggctttcag tacgtcgtaa gtctggtagg cgatgctttc cagcgcggcg cgtgagatat
     5101 gcgcctgggt cgttccgcgt gtcatgccga aaagcgtccc gcgcgcatac tggttccagt
     5161 gaggcgcgcc aaggcccgcg aaggccggga ccatgtagac accgtcggtg gtgtccacgg
     5221 aggctgcgag ttgctctatt tgtgccgagt cgcggataaa atgcagtccg tcgcgcagcc
     5281 attggacgac ggctccgcca atgaagatgc tgccttcgag cgcatattcg gtcttgcctt
     5341 cgcgggtcca ggcgatggta gtgatgagtt ggtttttgga atcgacggcc tttgaccctg
     5401 tgttcatgag caggaagcag cctgtgccgt aggtgttctt gaccatgcct ggctgtgtgc
     5461 aaagttgtcc gaacaatgcc gcctgctggt cgccggcgat gcccgatata ggaatgccgg
     5521 gggatccggg cgccatgatg ctggccgtgc ggccatagat ctcgctggag gatcgcacct
     5581 cgggcaggat atttcttgga atatcgaaga gtttcaggag atcgtcatcc caggcgcccg
     5641 tatggatatt gaaaagcatc gtgcgtgagg cgttggtggt gtcggtgatg tggaggcttt
     5701 tgttgctgag cttccacacg agccaggtat cgacggtgcc gaatgcgaga tcgcctttcg
     5761 ctgcactggc ccgtgcgccg gggacttcgt cgagtatcca tttgagcttg ctggcggaga
     5821 aataggcgtc gatgatcaga ccggtgcgtt cgcggatagt gggggtatgg ccgtcggtct
     5881 ttagcctgtc gcagtactcc gccgtgcggc ggtcctgcca gacgatagcg gggtggatgg
     5941 gttttccgct gctgcgttcc cagacgatga cggtttcgcg ttgattggta ataccgatgg
     6001 cggcgatatc ttttgtggta atgccagctt ttgcgacgac ctcggccatg actccgtatt
     6061 gtgatgacca gatctcttcc gggtcgtgtt ctacgcggcc gggctgggga tagaactggc
     6121 ggaattcttt ttgggccatc tgttgtatcc tgccgttgtg gtcgaatagg atggctctag
     6181 agctggtggt gccctggtcc agtgaaagga tataggaaga cggcatgtaa tgttttttga
     6241 aagatagtgt aatctgcaga aatgcgtagt tttaacggtc aaataattat cggctatgga
     6301 agagcgaaac gatcttatcg atctgagtca tacggtgatg gatgggctgg tcacgtataa
     6361 aggtttgccg gc
//
LOCUS       DASYSV010000460         5286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_219628, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000460 DASYSV010000000
VERSION     DASYSV010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219628"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VGQ51_14135"
     CDS             <1..287
                     /locus_tag="VGQ51_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037768.1"
                     /translation="PCYLPTWYFSLVRVKQRITQIKDSLFPSEFKGLSQREIFEIILK
                     SNQFGSEYHPDFLQWLPQTMLSNCGRSYKFNFTTIPLRSFETDWIIHTQE"
     gene            544..816
                     /locus_tag="VGQ51_14140"
     CDS             544..816
                     /locus_tag="VGQ51_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037769.1"
                     /translation="MSLNGGIVVVGKASAPLQFGRNWLLSPFVICWPGAASDFPMVRY
                     RVNYIGTEILGEDVDIEFQDPEYTKNIKLAVKHVGRELYSKLTAVD"
     gene            complement(1035..1772)
                     /locus_tag="VGQ51_14145"
     CDS             complement(1035..1772)
                     /locus_tag="VGQ51_14145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9037770.1"
                     /translation="MPSPAFIHVALVGDHALNLHALAFLLREQRGFRVVSISRDVTQL
                     RGSLEFAGKEFHPHVVLIDTNFDLHRAGVIISFLGRSHPEIRVAALGLTNDRRAIRCL
                     LQMGLHGYIAKNSDPNKLESMLRRLAAGEGETDAGKADMASMTSVQDRGSVIGQMRGN
                     LAPEGESDAGGLSVQGWPAVTVMERRYFRLTMAEAPEEDIRRSMRLPQSEFGRLVAGI
                     HRQFGVRSSHGLVLALYRNRFIVMDDI"
     gene            complement(1873..1995)
                     /locus_tag="VGQ51_14150"
     CDS             complement(1873..1995)
                     /locus_tag="VGQ51_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037771.1"
                     /translation="MLEAGARAVLLKSVEPEQLKEAMLRVLEDGFGPEGQCDRF"
     gene            complement(2346..3053)
                     /locus_tag="VGQ51_14155"
     CDS             complement(2346..3053)
                     /locus_tag="VGQ51_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV9037772.1"
                     /translation="MSLGGLFPIDKWDFKSQSVLRNLPEEDYALLIANQSVQTYRKGE
                     IIFREGSLPAGIFYIKSGKVKKYKAVEGKGEQIIYVANTGELLGYHAVLSQERFPDSA
                     ATLEESVIGFIPKEDFLEVVAQSTILSGRLLKTLSHEFTVFANGMALFGQRSVRERLA
                     LQLIVLREKYKLNFQPGMPVVIDLSREDLANLVGAVRENVVRILSEFKEEGIIETRGR
                     KILVKDVLKLIKIADYK"
     gene            3152..3814
                     /locus_tag="VGQ51_14160"
     CDS             3152..3814
                     /locus_tag="VGQ51_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV9037773.1"
                     /translation="MEISTGASTDLYITRDHKGIEFQGAVGSQARWFSSDMRLNDVYP
                     EPIQALASLHWTPLRIARLASRFLASHTGAKILDIGSGVGKFCLAGAYFQPRAQFFGI
                     EQRKHLVDHAESARSLLALRNVHFIHGNLTQLDFKLYDHFYFFNSFYENLNDTIKIDD
                     SVPCLPELYHYYNRNLIRKLHEMPAGTRVVTYHVLGVNMPPGFHIVEEHPDLFLTFWV
                     KT"
     gene            3825..4430
                     /locus_tag="VGQ51_14165"
     CDS             3825..4430
                     /locus_tag="VGQ51_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV9037774.1"
                     /translation="MYFVPASGSLAHFFESEENFRQLYPRPVQQLDAMHWSPLSVIEK
                     AAAFLAANKGAKILDIGSGSGKFCLAGSYYQSDAFFYGVEQRKNLVDHARHAAERSGM
                     TRVQFVHKDFTRLDLTQFDHFYFYNSFFEHLVHTDKIDDSIAYDPGLYDYYSKYLYSQ
                     LKMMPAGTRVVTYCSWNEEIPPGYELIRTGLNNFLKFWIKK"
     gene            4427..>5286
                     /locus_tag="VGQ51_14170"
     CDS             4427..>5286
                     /locus_tag="VGQ51_14170"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV9037775.1"
                     /translation="MKTGKVSIAGFGAVLCIATATSVPALQALLGHKTIEAGFSLPFP
                     FGTVPVRIDPLSAWFMLIINLTCINSALYGAGYLQKYAEQRTLLMLHWIYFIFFQTSM
                     LAVCVLQHGLAFLIAWEIMSISSFLLVVFEHWNPGTLKAGINYLVQMHLGVALLSIAF
                     IAIYSSEGSFDFASISTYFSQHDPDWVFLLFFVGFGIKAGFIPLHTWLPHAHPAAPSH
                     VSGAMSGVIVKLGIYGILRMITFLHSGLLVAGEAILVLSLLTALYGILNAAVHRDVKR
                     MLAFCTIENIG"
BASE COUNT         1291 a         1445 c         1195 g         1355 t
ORIGIN      
        1 aaccatgtta tttgcccacc tggtatttca gcttggtaag agtaaaacaa agaattacac
       61 aaatcaaaga ttcccttttc ccttccgaat ttaaaggctt gtcccagaga gagatttttg
      121 aaataatcct aaaatcaaat caattcggga gcgagtacca tccggatttt cttcaatggt
      181 taccccaaac catgttatct aattgcggca ggagctacaa attcaacttt actactatcc
      241 cgctccgctc ttttgaaaca gactggatta tccacactca agaataaaga acaccgtcca
      301 ccatggtcta tcccctcctc tttatcatac tattgatgcc gactccgttt ctggcttttt
      361 tgttcatata caggactatt gagaagaaag cgaaaaaaac cattctgctt cttcccgatg
      421 acgtcctcat taaatataca gataccagga tatggtttaa aggatatgac atggcaaaac
      481 gaccaaatcg gtttcaattt gatcttttaa aatcattgta cgtttacaac ctggcagacc
      541 tgtatgtctt taaacggggg tatcgtcgtc gtcgggaagg cgagtgcacc attgcaattc
      601 ggccgaaatt ggctgctttc acccttcgtt atttgctggc ccggggctgc atcggacttt
      661 cccatggtac gctatcgtgt caattatatc ggtaccgaaa tccttggcga agatgtcgat
      721 attgaattcc aggacccaga atacaccaaa aatatcaaac tggccgtcaa acacgtcgga
      781 cgagagcttt atagcaaatt aacagctgtc gattaagagc gattgtgtga ctgccgcacc
      841 catcggctcg accagcggct cgccgatatt gaaaaacaaa tcggttcgtt acagtcaaca
      901 ccaccagccc cagccgccac acccttggca gcctaacgca acttttcatc caggccgggg
      961 ctcccccgtc ctttttttat tgcttctcct atggggcaaa tagtagcagt cccgctaaat
     1021 gtccttcgca gcggctaaat atcatccatc acaatgaacc ggttccggta caacgccagc
     1081 accaacccat gactcgacct gacaccgaat tgccggtgaa tacccgctac cagccgtccg
     1141 aactccgact gcggaagtcg catggaccgg cgaatatcct cctcgggtgc ctcagccata
     1201 gtcaggcgga aataccggcg ttccattacc gtaacggccg gccagccctg cacagacaac
     1261 ccgcccgcat cggattctcc ttcaggagcc aggttgcccc gcatctgccc gatcactgaa
     1321 cccctatcct gcaccgacgt catagacgcc atatcagctt tccctgcatc cgtttcaccc
     1381 tcaccggctg ccagtctccg tagcatgctt tcgagcttat tcggatcact attcttggcg
     1441 atatacccgt gcaagcccat ctgcagcaga cagcggatcg cccgccggtc atttgtcaac
     1501 cctaaagcgg ctacgcggat ttccgggtgt gatcttccaa gaaaggaaat gatcaccccg
     1561 gcccgatgga gatcaaaatt ggtatcaatg agaacgacat gcggatgaaa ttctttgccc
     1621 gcgaactcca ggctgcccct caactgagtg acatccctgc tgatcgaaac gacgcgaaaa
     1681 ccgcgctgct ctctcaacag gaaagccagc gcgtgaagat taagggcatg gtcgccaaca
     1741 agcgcgacgt gaatgaatgc ggggctgggc atttgctccg gaagatagca ctgtgctcca
     1801 tcgaatattg cacgcagata cctctgtttg aaaatatatg gaaattattc acccgcccct
     1861 agagacatcc cattaaaaac gatcgcattg accctccgga ccgaacccgt cttccagcac
     1921 acgcaacatt gcctccttca actgctctgg ctcgacgctc tttagcagaa ccgcccgcgc
     1981 acccgcctca agcatccttc gtattttttt tcatcggcat tcagcgtgaa ccccacaatc
     2041 ctgatttgcg gagaataaac gtgagcgaaa ccgcgccaat taaggttagg aacgaataaa
     2101 atcttcaagc gaagtcgaaa agggaacgca gatctaacga ttttcgcctt taattcaaag
     2161 cattcgtacg ttccctagcg ggcgcccggc cacagcacct gctctgggcc agcacggacg
     2221 tcaaagacct ggcctatctg tttggccgca taggcgatag tttggccgga aaagtgatga
     2281 attacgattg gcgccggaga ctgtgccggg tcaaaaaggg taaggagcaa tcctggaaga
     2341 cccctctatt tataatcagc gatcttgatg agctttagaa catcctttac gaggatcttt
     2401 cttcccctgg tctcgatgat gccttcctct ttgaattccg aaaggatcct gaccacattc
     2461 tcgcggacag cgccgaccag gttcgccagg tcctcgcggg aaaggtcgat caccacaggc
     2521 atacccggtt gaaaattcag tttgtatttc tcccggagta cgatgagttg caaggccaac
     2581 cgctcccgga ccgatcgttg gccgaacagg gccataccat tggcaaaaac cgtgaactcg
     2641 tggctgagcg ttttcaataa ccgtccgctc agaatggtcg attgtgccac cacctccagg
     2701 aagtcttctt tgggaatgaa tccgatcacg ctttcttcca gggttgcggc ggaatccgga
     2761 aaacgttctt gtgacagtac cgcatgatac cctagcagtt cacccgtatt ggcgacgtag
     2821 atgatctgct caccctttcc ttcgacagcc ttatattttt tcaccttacc gcttttgata
     2881 tagaagatcc cggcgggcag tgaaccttcc ctaaagataa tttccccttt acgataggtc
     2941 tgcacgcttt gattagcaat taacaaagca taatcttctt ccggaagatt tctcaggaca
     3001 gactggctct tgaaatccca tttatcgatc gggaataatc caccaaggct cataagtatt
     3061 tcaaagttag gggattaaaa atgataaaag tcactttttt tattgacaaa attgcattgt
     3121 acggctctcg gatgcggaat aaatttgcac gatggagatt tctacgggcg cgagcacaga
     3181 cctatacatt acccgagacc ataaagggat tgaatttcag ggagctgtgg gctcacaagc
     3241 tcgatggttc agttccgata tgcgtttaaa tgatgtctac cctgaaccca ttcaagcgct
     3301 ggcctccctg cattggacac ctttgcggat tgcgagactg gcgagccgtt ttctggcttc
     3361 tcatacaggg gccaaaattt tggatatcgg cagcggagtg ggtaaattct gtctggcagg
     3421 agcttacttc caaccccggg cgcaattttt cggcattgaa cagcgaaaac acctggtaga
     3481 tcatgcggaa tcggcccggt cccttctcgc cctgcgcaat gtccatttta tccatggtaa
     3541 ccttacccaa ctggatttca aattgtatga ccatttctat tttttcaatt ctttttatga
     3601 aaacctgaac gatacgataa agatcgacga cagtgttcca tgcctgcccg aactatatca
     3661 ttactacaac cgcaacctga ttagaaaact tcatgaaatg cccgccggca cgcgggttgt
     3721 cacctatcat gtgctgggcg ttaatatgcc accgggcttt cacatcgttg aagaacatcc
     3781 ggatttattc ttaacattct gggtaaagac ttaattcccg acctatgtac tttgttcccg
     3841 cttcaggcag tcttgcccat tttttcgaga gtgaggagaa ctttcgccag ctctacccac
     3901 gcccggtgca gcaattggat gccatgcact ggtcgccact ttccgtcatc gagaaggccg
     3961 cggcattcct ggcagcgaat aaaggcgcga agatcctgga catcgggagc ggtagcggga
     4021 agttctgtct ggccggctcc tattaccagt ctgacgcgtt tttttatggg gtcgaacagc
     4081 ggaaaaacct ggttgaccac gcccggcatg ccgcggaacg atccggaatg acccgcgtcc
     4141 agtttgtcca taaagatttt acccggcttg acctgacgca attcgatcac ttttatttct
     4201 ataattcttt tttcgagcac cttgtacata ccgacaagat cgacgacagc atcgcctatg
     4261 atcccggatt gtacgattac tatagcaagt atttatacag ccagttgaag atgatgcccg
     4321 ccggcacaag ggtggtcacc tattgcagtt ggaacgagga gatccctccg ggatatgaac
     4381 tgatccgaac aggcctgaac aatttcctaa aattctggat aaaaaaatga agacagggaa
     4441 agtatccatc gcaggttttg gggcagtact ttgcatagca acggcaactt cggtgccggc
     4501 cttgcaggct ttactcggcc acaagaccat cgaagcaggg ttttccctgc cctttccgtt
     4561 tggaaccgtc cccgtccgga tcgaccccct ttctgcgtgg tttatgctta tcatcaacct
     4621 cacctgcatc aacagtgcct tatacggcgc aggctatctg caaaaatacg cggagcaaag
     4681 gacccttctt atgctccact ggatctattt tatcttcttc cagacatcca tgctagccgt
     4741 ttgtgtactc cagcatgggc tggccttttt gattgcctgg gagatcatgt ctatctcgtc
     4801 ctttcttctc gtggtgttcg agcattggaa ccccgggaca ctgaaagcgg ggatcaatta
     4861 cctggtccag atgcatctcg gggtggctct tctctccatc gcgtttatcg ccatctatag
     4921 cagcgaaggc tctttcgact ttgcttccat cagcacgtat ttttcgcaac atgatcctga
     4981 ttgggtcttc ctgctttttt tcgttggctt tggcatcaaa gccgggttta tccctttgca
     5041 tacctggctg ccccacgcgc atccggcggc gccctcgcat gtatcgggcg ctatgtcggg
     5101 tgtgatcgtc aaactgggaa tatatggtat cctgcgtatg atcaccttcc tccattcggg
     5161 gttgctggtg gccggcgaag ccatcttagt gctatcgctg cttacggccc tgtatgggat
     5221 actgaacgcc gcggtccacc gggatgtcaa acgcatgctg gctttctgca ccatcgaaaa
     5281 tatcgg
//
LOCUS       DASYSV010000461         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_733910, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000461 DASYSV010000000
VERSION     DASYSV010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733910"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VGQ51_14175"
     CDS             complement(<1..365)
                     /locus_tag="VGQ51_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV9037776.1"
                     /translation="MEIDNITYEDLSLFSKEEEYSIFHKLDFTRTRGGRDVLLKYFND
                     PFSKLESIRATQSILGLILEKLDEWPSSISNGTIMVMDRFYETAIDDIPHGQNLPAAL
                     SYKIFHTADFALVRYSVGH"
     gene            complement(384..1724)
                     /locus_tag="VGQ51_14180"
     CDS             complement(384..1724)
                     /locus_tag="VGQ51_14180"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV9037777.1"
                     /translation="MRNYLIKNIFIVNEGRITTGDLLLKNGRIEKIGTGLQPSFAVTE
                     INGEGKYLLPGAIDDQVHFREPGLTHKATIYTEARAAVAGGITSFMEMPNTIPPAFTH
                     GLLEQKYAIGASHSLANYSFYMGTSNDNAEEALRTNQRKKDVCGIKIFMGSSTGNLLV
                     DNYLTLDRLFRESEVLIATHCEEEKIIRRNLERLRAEGRELVPSDHPLIRDEEACFES
                     SLTAIQFAQKHNSRLHVFHLSTAKEMALFGNMIPLADKRITAEVCVHHLHFTSDDYPR
                     LGNRIKCNPAIKAPQNREALWQALLDDRLDLIATDHAPHTLAEKEVPYEKAHAGLPLV
                     QHSLLLMLHYVRLGKISLEKVVEKMSHAVATCFQIAERGYIREGYYADVVIVDQDQPS
                     VVRPDNILYKCGWSPLEGFEFPATVTHTFVNGHLVYGNGSFDESQKGLRLRFDR"
     gene            1743..>2548
                     /locus_tag="VGQ51_14185"
     CDS             1743..>2548
                     /locus_tag="VGQ51_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037778.1"
                     /translation="MTQFIKTPEVLRRFLQMLLVALVPATIHAQSGYLPQGSKFEHFL
                     DRLEILQQTNPELNFSTDKPISRKLAVRMSEMADSLDKFYPYDELYHLSSVDRATLAS
                     LLMNNPEWVRGGTDSFRSKRPWFNTFYKNKAIFFLVQDKDFFLALDPVIQETQSIESG
                     NHSRVFLNTKGATFRGMIANKIGFNVYLTDNQERGPSYFQQRAVQEDAMPGAGYFKHF
                     HTTGYDYYDNRASIYFNTWKYFDWTFGYDKNFIGDGYRSLFLSDYSAPYL"
BASE COUNT          600 a          684 c          626 g          638 t
ORIGIN      
        1 aaatgcccca ccgaatatct cacaagcgca aaatcggcgg tgtgaaagat cttataggaa
       61 agcgccgcgg gcaggttctg cccatgcggg atatcgtcga tcgccgtttc gtaaaatcgg
      121 tccatgacca tgatggtccc attgcttatg gaagacggcc attcatccag cttttccagg
      181 ataagaccca ggatggattg ggtcgcccgg atggattcca gcttactgaa tggatcgttg
      241 aaatatttca gcaggacatc cctgcctccc cgtgtccggg taaagtccag tttgtgaaag
      301 atcgagtact cctcctcttt gctgaagaga gacaggtctt cgtacgttat attatcgatt
      361 tccatggtat gagtgctgtg atcttaccgg tcgaaccgca accgcagacc cttttgagat
      421 tcatcgaagc tcccatttcc ataaacgaga tgcccgttca cgaaggtatg ggtcaccgtt
      481 gcagggaatt caaagccttc caatgggctc cagccgcatt tgtaaaggat attgtccggt
      541 cgcaccaccg aaggctggtc ctgatccacg atgaccacgt cggcataata accttcccgt
      601 atataacccc tctctgctat ctgaaaacag gtggccaccg catgactcat tttttccacc
      661 accttttcaa gactgatctt ccccaaccgg acatagtgaa gcatcaacag cagcgaatgc
      721 tgaacgagtg gtaacccggc atgagctttt tcatagggga cctctttctc cgccagagta
      781 tgcggcgcat gatccgtagc tatcagatcg agacggtcat ccagcagcgc ctgccacaac
      841 gcttcccggt tttgaggagc ctttatggcg ggattgcatt tgatcctgtt ccccaaccgt
      901 ggatagtcgt cactcgtaaa atggagatga tgtacgcaaa cctccgccgt gatccgtttg
      961 tccgccaggg gtatcatgtt tccgaaaagt gccatctcct ttgctgtgga aagatgaaag
     1021 acatggagcc tgctgttgtg tttctgcgcg aactgtatcg cggtcagcga cgattcgaaa
     1081 caagcttctt catcccggat cagcggatga tccgacggta ccaattcccg gccttcggca
     1141 cgcaaacgtt cgagattgcg ccggatgatc ttctcctctt cacaatgggt agcgataagc
     1201 acctcagact cgcggaacag ccggtccagg gtcaggtaat tatccaccag caagttcccc
     1261 gtggacgaac ccatgaatat cttgattccg cagacatcct ttttccgctg attggtacgc
     1321 agggcctcct ccgcattgtc attcgaggtg cccatataga aggagtagtt cgccagcgag
     1381 tggctcgcac cgatagcata tttctgttcg agcagcccgt gggtaaaagc cggcggaatg
     1441 gtattgggca tttccatgaa agaggtgatg ccgcctgcca ccgccgccct ggcctcggta
     1501 tagatggtag ccttatgggt tagacccggt tcacgaaaat ggacctggtc atcgatagcg
     1561 ccgggcaata aatatttgcc ttccccgttg atctccgtta cggcgaagga aggttgaaga
     1621 ccggtgccta ttttctcgat ccgcccgttt ttgagcagca aatctccggt ggttatccta
     1681 ccttcgttaa cgatgaatat gttcttgatc agataattac gcatactgaa ctgcctcgct
     1741 ttatgacgca atttattaaa acccccgaag tactcaggag atttttgcag atgctgttgg
     1801 ttgccttggt gccggcgacg atccatgcac aaagcgggta tcttccgcag ggttctaaat
     1861 ttgaacattt tctggacagg ctggagatcc ttcagcagac caatcccgag ctcaatttca
     1921 gtacggacaa gcccatcagc cgtaaactgg ccgtccggat gtccgaaatg gccgatagcc
     1981 tggataaatt ctatccctat gacgaactgt accatctgtc gtcagtagac cgggctaccc
     2041 tggcttccct gctgatgaat aacccggagt gggtgcgggg cgggacggac agtttcagga
     2101 gcaagagacc ctggttcaat actttttata agaacaaggc gatctttttt ctcgttcagg
     2161 acaaagactt cttcctggcg ctggatcccg tcatccagga gacgcaatcg atcgaaagcg
     2221 gcaaccattc ccgcgtcttt ttgaatacca agggcgccac tttcagggga atgatcgcga
     2281 acaagatcgg gtttaatgtc tatctcaccg ataaccagga acggggcccc tcgtattttc
     2341 agcaacgcgc cgtccaggaa gatgccatgc ccggcgccgg ttatttcaaa cactttcata
     2401 ccaccggcta cgactattat gataaccgcg cctccatcta tttcaacacc tggaaatatt
     2461 tcgactggac attcggatac gataagaatt tcatcgggga tggctatcga agcctgttcc
     2521 tcagcgatta tagcgctccg tatctctt
//
LOCUS       DASYSV010000462         5238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_477417, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000462 DASYSV010000000
VERSION     DASYSV010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477417"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..995)
                     /locus_tag="VGQ51_14190"
     CDS             complement(138..995)
                     /locus_tag="VGQ51_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEV9037779.1"
                     /translation="MIHIVFQSGDIVALKKAFELDPSFQGEVVCIEDDFAVGPIANIY
                     SAEGIEARKQWWSVVLAGGDYENRLAPGGAGDDNGKVAALKQRLSEDQKEIVWIWAAQ
                     NKHDVSGYYWLMSQLKEFQGRIFILYLNNLPFINEKGLIFYPENLFQIPPREFLKARK
                     LARPITLSEFEVDPDEWTRLSAEDKGVRILEGGKKLSQHDYVYYDEELRKFITGDWIR
                     VSKLIGQFLNKARQTTGDAYLLWRIKQMVVAGDLDVQGELKGMRDFEIKSKNGMPVVE
                     ESVPADAAN"
     gene            complement(1001..2893)
                     /locus_tag="VGQ51_14195"
     CDS             complement(1001..2893)
                     /locus_tag="VGQ51_14195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV9037780.1"
                     /translation="MAEKNNLFEYNEDSVRTLDWKEHIRLRPGMYIGKLGDGSSPDDG
                     IYVLLKEVIDNCIDEYTMGYGKQVEITLDKRSVTIRDYGRGIPLGKVVDVVSKINTGA
                     KYDSKAFQKSVGLNGVGTKAVNALSSFFHVTAFRDGKKKSAEFERGDLKIEHKEAKTD
                     EPNGTKVIFVPDEKVFKNFHFLNEYMDNQFWNYCYLNAGLVINFNGKKYVSKNGLLDL
                     LQRRTNEDELRYPIIHLKGDDIEVALSHNNDYGEEIFSFVNGQYTTQGGTHQQAFREA
                     FVKVIRDFYKKDYDAADIRQSIVGAVSVRVIEPVFESQTKTKLGSLNVSEGGATMKNF
                     LGDFLAKELDNYLHKNPATAEALKKRIEQSEHERKELAGIKKLANERAKKANLHNKKL
                     RDCRYHYNEEPTGKDKAQVLEKQKESTIFITEGDSASGSITKARSVETQAVFSLRGKP
                     LNCFGLTKKIVYENEEFNLLQHALNVEEGIEGLRYNNIVIATDADVDGMHIRLLLMTF
                     FLQFFPDLVKNGHIYILETPLFRVRNKQETIYCYDEAEKQAAIKKLGGKPEITRFKGL
                     GEISPDEFARFIGPDMHKQPVMIMHDTHIQQVLEYYMGKNTPDRQEFIIQNLKVELDL
                     VEEGKA"
     gene            3060..4586
                     /locus_tag="VGQ51_14200"
     CDS             3060..4586
                     /locus_tag="VGQ51_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9037781.1"
                     /translation="MSSSFLTLEGISVRIGDILLLDHLDWTIRRGEQWAITGASGSGK
                     TLLAHVLLGRQFAAGRISGPPGQRIAIVEQQHRFRNNAGATQLYYQQRFNASDADQTI
                     TVAEELAEAGWTGHPFPDALHILPLLHKPLIQLSNGENKRVQLAIALLGEPELLILDQ
                     PFPGLDTEGRATLHMIIDGLAARGIQLLLITAPHELPTSVTHVAQLEKGRWVFRGPKS
                     DFHPADLQTVTVKLDPEILSELRSAALREENPTPVQQENVSSLRNEDPGFSFAVRMVN
                     ATVRYGEKTILRNINWEVRKGQRWNLAGHNGAGKSTLLSLITADNPQAYANDIWLFDR
                     RRGTGETIWEIKKKIGFVSPELHLYFDTSATVFEVVASGLFDTIGLFRMLSAKQEALV
                     GHWLRLLSLHDLRTKRLAQLSAGQQRRVLLARALIKNPPLLILDEPCQGLDEEQTAWF
                     RELIDALCRAFDTTLIYVSHYIRELPSCIDRFLLLENGEATESNGNEHSKRRQDQAGM
                     "
     gene            4741..5058
                     /locus_tag="VGQ51_14205"
     CDS             4741..5058
                     /locus_tag="VGQ51_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEV9037782.1"
                     /translation="MNFTSLKLSAVAIAAAGLFAFAHLKSGSIRGTIMPPDAGVRAWA
                     ESATDTLKAPIVNGSYEITDAKPGAYKIIIEAKPPYRNAAKDGLMVSDGQSTDAGEIR
                     LEK"
BASE COUNT         1132 a         1547 c         1269 g         1290 t
ORIGIN      
        1 ccaccgccgg aacggcccgt tccaaaatga cgtaggaagc atagtccagg aaccaattct
       61 tatactggcc ggtgacgccg gactcatttc cgtgaaagtc ttccgttatt ttactcttcg
      121 ccataattcg attgttgtta attcgccgca tcggccggaa cggattcctc gactaccggc
      181 ataccgttct tcgatttgat ctcgaaatcc cgcatgccct tcaactcgcc ctgtacatcc
      241 aggtcgccgg cgacaaccat ttgtttgatc cgccatagca ggtaggcgtc accagtggtc
      301 tgtctggcct tgttaaggaa ctggccgatg agcttgctga ccctgatcca atcccccgtg
      361 atgaattttc tcaattcctc gtcataataa acataatcat gctgtgacag cttcttcccg
      421 ccttccaata tccgaacgcc tttgtcctcg gcgctgagcc tggtccattc gtcgggatcg
      481 acctcgaatt cgctgagcgt tatcggccgg gccagtttgc gggccttcag aaattcgcgc
      541 ggaggtatct ggaacagatt ctcaggatag aagatcaggc ccttctcatt gataaaggga
      601 agattattga gatacagaat aaagatgcgc ccctggaatt ccttcagctg actcatcagc
      661 caataataac cgctgacatc gtgcttattc tgcgccgccc atatccagac tatctctttt
      721 tggtcttcgc tcagccgctg tttcagcgcg gcaaccttcc cgttgtcatc accggcgccc
      781 cccggggcca gccggttttc ataatcgccg cctgccaata ccacgctcca ccattgttta
      841 cgtgcttcga tgccctccgc ggaataaatg ttcgcgatgg gcccgacggc aaaatcgtct
      901 tcgatgcaca caacttcccc ctggaaggat gggtccagct caaatgcctt tttcaacgca
      961 acgatatcac ccgattgaaa aacgatatgg atcatacgta ttatgctttt ccctcctcca
     1021 ccaggtccag ctcgaccttc agattctgaa tgatgaattc ctggcggtcc ggcgtgttct
     1081 tgcccatata atattccagc acctgctgga tatgcgtgtc gtgcatgatc atcaccggct
     1141 gtttgtgcat atccggccct ataaaccggg cgaactcgtc cggagatatc tctcccagcc
     1201 ccttaaaccg ggtgatctca ggtttgccgc ccagcttttt tatcgccgcc tgtttttccg
     1261 cctcgtcata acaataaatg gtttcctgtt tgttgcggac ccgaaaaaga ggggtctcga
     1321 ggatatagat atgcccgttc tttacgagat cggggaagaa ctgaaggaaa aaagtcatca
     1381 gcagcagacg gatatgcatt ccgtccacat ccgcatccgt cgcaataacg atattgttat
     1441 atctcagacc ctcgatcccc tcctccacat tcaacgcgtg ctgcagcagg ttgaactctt
     1501 cgttctcgta aacgatcttt ttggtcagac cgaaacagtt gagcggcttg ccccggagac
     1561 tgaagaccgc ctgcgtctcc acgctgcgcg ccttggtgat gcttccgctg gccgaatctc
     1621 cctcggtgat gaagatcgtg ctttcctttt gtttctccag cacctgcgcc ttgtccttgc
     1681 cggtgggttc ttcgttataa tgataccggc aatcgcggag tttcttgttg tgcagattcg
     1741 cctttttggc cctctcgttc gccagcttct tgatgcctgc cagctccttt cgctcatgct
     1801 cgctctgctc gatccgtttt ttcaacgcct ctgccgtcgc cgggttcttg tgaagataat
     1861 tgtccagctc tttggcgagg aaatcgccca ggaagttctt catcgttgct ccgccttccg
     1921 atacgttcag gctccccagc ttggtcttgg tctgggattc gaacaccggc tcgatcacgc
     1981 ggacggagac ggccccgacg atgctttggc ggatatcggc cgcatcgtag tcctttttgt
     2041 aaaagtcacg gatgaccttg acgaaggctt cgcggaaggc ctgctgatgg gttccgccct
     2101 gcgtggtata ctgaccattg acgaagctga agatctcttc cccgtaatcg ttgttatggg
     2161 ataacgcgac ctcgatgtca tctcccttca gatggatgat ggggtaacga agctcgtctt
     2221 cgttggtacg gcgctgcagc agatcgagca agccgttctt cgagacatat ttcttcccgt
     2281 tgaaattgat caccagtccc gcgttcaggt aacagtagtt ccagaactgg ttgtccatgt
     2341 attcattaag aaaatggaaa ttcttgaaaa ccttttcatc tggcacgaaa ataactttcg
     2401 tcccattcgg ctcgtcggtc tttgcctcct tatgctcaat tttaaggtcc cccctctcga
     2461 attctgccga tttctttttt ccatcccgga aagccgtcac atggaagaaa ctgctcaacg
     2521 cgttcactgc cttggtaccc acgccattaa gtcccaccga tttctgaaac gccttgctgt
     2581 cgtatttggc gccggtattg atcttgctga ccacatccac aacttttcca aggggaatgc
     2641 cccggccata gtcgcggatc gtgaccgaac gtttgtccaa cgtgatctcc acctgtttgc
     2701 cataacccat ggtgtattcg tcgatgcaat tgtcgatcac ctctttgagg aggacataga
     2761 tgccgtcatc ggggctgctg ccgtcgccca gctttccgat gtacatcccg ggacggagac
     2821 ggatatgctc tttccagtcc agcgtcctga cgctgtcctc attatactcg aacaaattat
     2881 ttttttctgc cataaattaa ttcaaaggga cgacccgggc gccgtatagc cctgtccgca
     2941 aggccgcaat atactcaaac gtacgcacag aaggattatt tctaatctac acctgtggat
     3001 atcgcgccac atcccgatcc caccggaggg ttttccacct tctgtcctac attcgcacca
     3061 tgtcttcatc attcctgacc ctcgaaggga tctcggtccg cataggggac atcctgctgc
     3121 tggatcatct ggactggacc atccgtcgcg gcgaacaatg ggcgatcacc ggtgcttccg
     3181 gcagcggcaa aaccctgtta gcccatgttt tactcggccg ccagtttgcc gcaggccgca
     3241 tctccggccc acccggacag cgtatcgcca ttgtggagca acagcaccgg ttccgaaaca
     3301 acgccggcgc cacgcaactt tattatcagc agcgtttcaa cgcttcggat gccgatcaga
     3361 ctatcaccgt tgcggaggag ctggccgaag ccggatggac cggccaccca tttccggatg
     3421 cgctgcacat cctgcctctc ctgcataaac ccctgataca gctgtccaat ggcgagaaca
     3481 aacgggtgca gctggcgatc gccctgctgg gagaaccaga gctgctgatc ctcgatcaac
     3541 catttcccgg actcgatacg gaaggccgcg ctacgctcca tatgatcatc gacggtctcg
     3601 ccgcccgggg catccagctg ctcctcatca cagcgcctca tgagctaccc acctccgtta
     3661 cccatgtcgc gcagctggag aaaggccgat gggtcttccg gggacctaaa agcgattttc
     3721 atcccgccga cctgcagacc gtcaccgtga agctggatcc ggagatcctc agcgagctgc
     3781 gatcggccgc gctccgggaa gagaatccga ccccggttca acaagagaat gtctcctcgc
     3841 tccggaacga agatccgggt ttctctttcg cggtgcggat ggtaaacgct accgtgcgat
     3901 acggcgaaaa gacgatcctt cggaatatca actgggaggt ccggaaggga caacgctgga
     3961 atctcgccgg gcataatgga gcaggaaaat ccacgctcct gagcctcatc accgcggaca
     4021 acccgcaggc ctatgccaat gatatctggc tattcgatcg ccggcggggc accggcgaga
     4081 cgatctggga gatcaaaaaa aagatcgggt tcgtatcccc cgaactgcac ctgtatttcg
     4141 atacctctgc gacggtcttc gaggtggtcg cttcgggtct cttcgacacc attggactat
     4201 tcaggatgct ctccgccaaa caggaagcgc tcgtcggtca ttggttgcgg ctgttgtcac
     4261 tgcacgacct gcgcaccaag cgtctggcgc aattatccgc cggtcaacag cgtagggtcc
     4321 tgctcgcgag agcgctgatc aagaaccctc ccctgctcat tctcgatgag ccctgccagg
     4381 gacttgacga ggaacagaca gcatggttcc gggagctgat cgacgccctt tgccgggcct
     4441 tcgataccac cctcatctat gtcagccact acatccggga gctgccttcc tgtatcgatc
     4501 gcttcctgct gctggagaat ggagaggcga cggaatctaa cggtaacgag cattcgaaac
     4561 gaagacagga ccaggctggc atgtagaata ttttccacgc ccatggcgca tttcagacga
     4621 cgcagcgcga tctttaactt ttttatggca tagtccttgc aaacctattc atgaccctcc
     4681 ctcccatcgt gcagacatgg aaaacgagag actgatcatt aacattttaa atgccgggtt
     4741 atgaacttca cttcacttaa attatccgcg gtcgcaattg ccgccgctgg cctgtttgcc
     4801 tttgctcacc tgaagagcgg ctcgatcagg ggcacgatca tgcctcccga tgcgggtgtc
     4861 cgcgcctggg ccgaatccgc aacggatacg ctcaaagctc ccatcgtaaa tggctcgtat
     4921 gagatcaccg atgccaaacc gggagcctac aagatcatta tcgaagcaaa acccccttac
     4981 cgcaacgcgg ccaaggacgg actcatggtc agcgatggcc aatccactga tgccggtgaa
     5041 ataagactgg aaaaatgagt gatgatgctg taggataagt ggtcatgctg gattccggca
     5101 atgccggaac ccatgaagcc accctgtaga accggggtgg ctttcttgtt taataacata
     5161 ttgattatca atatattaat tcgctctatt tgcaaaacta tctcccagcg tctaaaagtt
     5221 agccgccggg aagtccaa
//
LOCUS       DASYSV010000463        23504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2399325, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000463 DASYSV010000000
VERSION     DASYSV010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 23504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23504
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399325"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /gene="dgt"
                     /locus_tag="VGQ51_14210"
     CDS             complement(<1..536)
                     /gene="dgt"
                     /locus_tag="VGQ51_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEV9037783.1"
                     /translation="MNWQQLYSPVRTGGGPRRKGGEGSEARTSFLRDYDRIIFSSAFR
                     RLQNKTQVFPLPGSVFVHNRLTHSLEVASVGRSLGRAVGDALSEKYPDAGGEFLEFYR
                     YELSSVIGAACLAHDIGNPPFGHSGEEAIRIFFREELRGEAQKRFEGELSLNRQRDFL
                     YFEGNANAFRTLTHDFNEP"
     gene            complement(547..1461)
                     /locus_tag="VGQ51_14215"
     CDS             complement(547..1461)
                     /locus_tag="VGQ51_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037784.1"
                     /translation="MTRFLLKTFFFLLPVIIGFGILSRLGVPPMMTNSGFFDHKMQWL
                     QRHPLKDVRLMAAGSSMTAYALNSEMIVEQLHLPYYNFASWSMQIGDTRVVVMDLVRT
                     YHPDYLVICSSPAEFTEADNPTYRNYTATSPFIREHFPGYFYFSDYHSIHQLLLRKYN
                     SSHLGIDEWGRGKALFEMGKKTLHGDDSARADPNVYFSEHASGFEHQYRELDSLCAWL
                     QQERVKLVFAQAPMQAFVGRNGRQWLNAHFDSCRLIVGRYGGVYLNYYDTTLFKDSLF
                     VDPGHLTAAGSDIITKKLVADLKIVIRQ"
     gene            complement(1478..2824)
                     /locus_tag="VGQ51_14220"
     CDS             complement(1478..2824)
                     /locus_tag="VGQ51_14220"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEV9037785.1"
                     /translation="MLFTTLPFFLFFVAFFILYWFVFAGTIKAQNGLILAGSYFFYGW
                     WDWHFLFLLAGTSLAVYFLGAWLCRTAREGRKNLLLGVGLLISLGSLLYFKYFHSLLP
                     LGLSFYTFKMISYLVDIRRGKTAPAGDVVVFCSYIAFFPCLLAGPIDRPASLIPQLER
                     RREFRYSAGSDGMRQILWGIFKKVVIADNCASFSSDIFAHYASLPASALVYGAFMATI
                     WVYADFSGYSDMAIGMSRLLGFTVSPNFNYPFLATNIAEFWRRWHITLTSWMTDYVFT
                     PLNIFLRDLGKGGLILAVLINFTLIGLWHGPSWTYVFFGLLNGCYFIPMLLRGFRRRR
                     AAGIGRIATFLLVMVSIILFRSVSLDQALGFYRRIFQTSLFSPFFIFEKVNTVVTLVC
                     ILGMFVAEWAQRGKPHVLQIDGIRAFPVRALIYFGLMGIILTFAATKNADFIYFRF"
     gene            complement(2826..3212)
                     /locus_tag="VGQ51_14225"
     CDS             complement(2826..3212)
                     /locus_tag="VGQ51_14225"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9037786.1"
                     /translation="MQLHHIGVACRDIEEEIGNICRVHEVLERTPIVYDPCQEATVAM
                     LTLADGTRIELVAGAPVQTLLRKGIGYYHLCFEVGDIESEIARLVLQNALLLSPPKPS
                     VLFGGRKVAFLQASYGLVELLSANKD"
     gene            complement(3218..3460)
                     /locus_tag="VGQ51_14230"
     CDS             complement(3218..3460)
                     /locus_tag="VGQ51_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV9037787.1"
                     /translation="MDKKTILETINSIFIEVLENRSIVLTPETTAGDVAEWDSLTHIQ
                     LVVAIEKKFGVRFTSREIQSWKNVGQLVDTLSTKLG"
     gene            complement(3453..4370)
                     /locus_tag="VGQ51_14235"
     CDS             complement(3453..4370)
                     /locus_tag="VGQ51_14235"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV9037788.1"
                     /translation="MTRIVDTITAKIAAQDPLHGKRLMKNLAVQDDRYHRLAEAFFQK
                     YEAILNREGKHIDYAVDCYLRMVEDVMTETIEFARTGKYTSSTFEEVNKRVYGRPETM
                     QYYMHGLILSQFLWRHHYEMFDYYSTVLPRYTGGVLHYLEVGVGLGFYLSHAREALSS
                     GASLTAVDISETSIALSRKFAGEDRIDYRLHDIFTFEAEQKYDLIVLGEVLEHVEQPL
                     ALLLRLKELLADDGVLFFTTPANAPAIDHLYLFHNVEEIRRMVRSAGFSIGSEKALPA
                     EDVSWEKVEQLKVAVLYGAFLTKQSDKHG"
     gene            complement(4379..6058)
                     /locus_tag="VGQ51_14240"
     CDS             complement(4379..6058)
                     /locus_tag="VGQ51_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEV9037789.1"
                     /translation="MCEVSAVRVVLLGDTSTQLLARLLSSAGAGCGLLLSVTEAGFDQ
                     IEREVLDPDSELYRRDPQVIILFHSTQRLLEIYDRLSPGEQDQLADDRLRLVDELKSA
                     VHARCKASLIYYNYPETDDAVFGNHSNKTERSFLFQLRKLNYGLMTYAAGQPGFYIVD
                     LATIQNRIGRPALYRPMLYIHAAMAVSEALPAVVAATAACIGALLGRSKKCLVLDLDN
                     TLWGGVIGDDGMEHIEIGSLGIGKAFSALQYWIKKLARRGIILAVCSKNTESIALQPF
                     REHPDMVLGLDDIAIFRVNWNSKVDNLRDIQAVLNIGFDSMVFLDDSPFEREMVRAAI
                     PDITVPELPEDPADWLEFLYGLNLFDTSSRSDEDAERTKLYQTEALRAVERRDYAGEE
                     AFLSGLEMVSRWEPFNTFNSPRVAQLCQRSNQFNLRTVRYTEMDILRMAGEPDRFTFA
                     FSLDDRLGAYGIVCLVILETVSDETLFIDTWLMSCRVLQRGMEQFTLNSIVDFARKRG
                     FRFLKGEYLPTPKNQLVRGHYEKLGFQPGDAYWLLDTGTFQTLKTEIRSAS"
     gene            complement(6073..6930)
                     /locus_tag="VGQ51_14245"
     CDS             complement(6073..6930)
                     /locus_tag="VGQ51_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV9037790.1"
                     /translation="MKLYRTRRGTLVNDGQDYRLLDQPWEQLINRSELHRYLRSLLSG
                     AATLPAAEADALLKGQLMAPIGTGQEVWAAGVTYLRSRDARMEESAESGGATFYDKVY
                     VAERPELFFKALPHRVAAHCEDVYIRRDSSWDVPEPELTLFINSNGEIQGYTIGNDMS
                     SRSIEGENPLYLPQAKVYDRSAALGPCLLVPAEPLPSSTRISMVIRRNGASVYSGEVA
                     ISQIKRRLPELAQWLYREMDFEYGCFLMTGTCLVPSQEFTLQEGDSIEIGIDGIGTLV
                     NRIGSRPIK"
     gene            7071..8897
                     /locus_tag="VGQ51_14250"
     CDS             7071..8897
                     /locus_tag="VGQ51_14250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9037791.1"
                     /translation="MKETASDTVEPHSFSLRNLSIRKQLPLLICLLLLILILLFGTIS
                     YISIRRATMAIGQQRVRSLTEQLASLFQQSAHTLASGAQRAADKPEIVRFLSIQYDKG
                     ASPNPGETLPTLQRLLADSQTVLAELRDLQGNILLRAGRQIGSSGETGSPSGPFAAGE
                     VAGRTDSNFVGKFYFSEDSIYYPVIATVRRDGRSIGYLYRWRPLRATPKSLAQLSQLM
                     GSKAALYFGNHDGSLWTDMLKPVAAPPQVPNDSHRIVTYTRPGGRVIADATPVAGTKW
                     IVLVELSSDLIMEASNRFLFWMILIGVVLILTGSFIAWLISRNFTRPLDRLIHATTAI
                     AAGTYSPIVVADRRDEIGKLARSFDSMAVQVRHAHDELENKVSIRTKELEVVNKELEA
                     FSYSVSHDLRAPLRAVSGYAMMLKEDYEDQFDDEAKRITGNILSNVKMMGRLIDDLIA
                     FSRLGKREVRRKITDMQALTETCVAELKQGWPEDKFLISVDPLPPCKGDEDLLKQVWM
                     NLIGNAMKYSAKNPDPRITIGATHNGDAVHDIYFVRDNGAGFDMKYADKLFKVFQRLH
                     SQEEFEGTGVGLALVKRILDKHQGEIWAESKPGEGTVFYFKL"
     gene            8911..9363
                     /locus_tag="VGQ51_14255"
     CDS             8911..9363
                     /locus_tag="VGQ51_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9037792.1"
                     /translation="MLNHVEVLLVEDNIHDAEMTIRALKKVNLANHLVHVKDGAEALD
                     FIFARGNFSGRDSEGLPKVILLDIKMPKVDGIEVLRQLKSSELSQTIPVVIMTSSKEE
                     QDIISSYKLGVNSYVVKPVDFEGFARAVSQLGLYWLLTNQPPMNDNQP"
     gene            9375..10784
                     /locus_tag="VGQ51_14260"
     CDS             9375..10784
                     /locus_tag="VGQ51_14260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9037793.1"
                     /translation="METLKILHLEDLPADAEMVERVLRKANFRFEKKVVVDRPDFVRA
                     LHEFRPDIILSDHSLPAFNSLEALRITREEGISAPLILVTATVSEEYAVNVIKEGASD
                     YILKDRLERLPNAVQNALDKCLMERQRRQADEALRASERKYKLLFEGNPMPMWMFSLK
                     SMRIIDVNEAATEHYGYTKEEFMSVGTSQIWQGANPGVYPSGIWKHKKKDGTIIDVEV
                     ITHDLLQGQEPVLLILAIDITDRLRAEVELSRQRDTQQKLITQTSIQVQEKEREQIGK
                     ELHDNINQILAATKLYLDIAMKESEEEQVTEMLLKSHKNLQSAMEEIRQLSQSLVAPS
                     LGGVTLDLALARLIENLRLASPLNLSLDTSGYRGDVSDEDMKLTCYRIVQVQLNNIIK
                     HAHAKDAVIQLKKGDMLELTIRDNGIGFSPGKNTAGIGIRNIRNRVGFYNGEVAVLSE
                     PGKGCTLKVTIPIPPTSPG"
     gene            complement(10759..13761)
                     /locus_tag="VGQ51_14265"
     CDS             complement(10759..13761)
                     /locus_tag="VGQ51_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEV9037794.1"
                     /translation="MLKALLFIALSTSVSLVARAQSIPYTSCPGCWNPDSLGNHRAAI
                     DFRGPGAVAHVNLPWRLRVADPAAHRIIVQDAVTGARVRNATTGVLTRESGGVFFEPV
                     SGKGLYYVYYLPYRNEGRSNYPKGVYWRPDTTAAPQWLAALNRTGPVNCTVTAFQSID
                     SFNTWYPMEVIATAKETAALKQKYAGKTFLVFPEDREHSIRMRDDLPYRWIAKGPGNA
                     VSGNASRGEWFAWQLGVYALKDIQNVRVNFSALTGPGGVMLDKISCINTGGISNDGKP
                     FSKDVSVSAGQVQALWCGVDIPRDIASGVYRGVVTVIAGGAQWKGQVTLRVGEQQVSD
                     GGAATPAKMTRLKWLNSTLAQENTVIAPYTPLTISGDTLIGLLGRDIRLTAQGFPEQI
                     RTFFTPEMTSIGTRPNSLLAENIHFHFTRASDGKDIRLKGPGVHFTERTPGTVRWEAT
                     STSDEVRMDVSASLEFDGFLAYTVRVTALQDLELKDITMHIPFEKAATTYMMGLGRKG
                     GYRPDSMYHWKWDVAHKNQDGAWIGTVNAGLQFSLRDERYVRPLNTNFYLQKPLIDPS
                     SWDNGGNGGIDIGVKGKSMLANNYSGHRLMKKGDTLYYNFNLLITPFHPLITDAQWAM
                     RFYHKYDDPKRVAATGATVINIHHATPINPWINYPFIEWRRMKGYIDTAHALGLKVKI
                     YNTVRELSDHAYELFALRSLGHEIYSAGPGKGFSWLQEHVEDDYIPAWFVPEIKDAAI
                     INSGMSRWHNYYVEGMNWLVKNVGIDGIYLDDVAFDRVTMKRIKRVLTEDGHPGIIDL
                     HSANQYNPRDGYNNSANLYMEHFPYLNRLWFGEYFDYEHNPPDFFLTEVSGIPFGLMG
                     EMLQGGGNPWRGMVYGMTNRMPWSDNADPRPIWKAWDEFGMQGTEMIGYWSPSCPVNT
                     GRGDIPVTVYRKKGAALIALASWAPEDTVIRLNIDWQRLGIDPAHATIEAPAITNFQP
                     ARRFAPDEPIPVEKAKGWVLIIRGSSGG"
     gene            13860..14861
                     /locus_tag="VGQ51_14270"
     CDS             13860..14861
                     /locus_tag="VGQ51_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037795.1"
                     /translation="MSPIAIYHEHPDWFRPLFNELDNRGIPYTRLNPAAHQFAIEAPA
                     PDYALFFNRMSPSAYLREGTQGIFYTLNYLKYLEYHQVPVVNGHKAWTFETSKAMQLM
                     LMQSLGIPYPRAHVVNHPLQLIAAAEGLRFPVVVKANIGGSGAGITKYQTLEDLRGAA
                     AGEMNFGVDHTALLQEFIPARGGYITRVETLGGKFLYAIRVHLTGDTFNLCPADICQT
                     STGVELVRNACALEAPKSGLKVEGYTPPSTVIHAVETLVQHSGIDVGGIEYIVDDRDG
                     ALLYYDVNALSNFVADAVNVIGFNPHEKLVDYLEKRAGIRKGDAFAKKNAHRPHANF"
     gene            14929..15963
                     /locus_tag="VGQ51_14275"
     CDS             14929..15963
                     /locus_tag="VGQ51_14275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV9037796.1"
                     /translation="MRYGYWMPVFGGWLRNVDDENMQPTWDYVKKLAIRSEEIGFDLS
                     LIAELNLNDIKGEHAPSLDAWSTTAALAAVTRKQELMVAVRPTFHNPALLAKQAANID
                     HISNGRLSLNVVSSWWRDEATKYGVGFEQHDDRYARTAEWLTVLNGVWNQDGFTYKGK
                     FYQVTDNTLQPKPVSRPRPQIYAGGESEAAKDLISSTCDGYVMHGDEPATIGARIRDL
                     KERREKKGLPQMRYGVAAYSIIRNSDAEVKRELARITDVRPGSPGYHNYQQWLAGTQL
                     EQKVSLEDYSVSNRGLRSGLIGSPAQVQDRIGEFADQGVDFFLLQCSPQLEEMERFAD
                     AIITEQYAKA"
     gene            15960..16847
                     /locus_tag="VGQ51_14280"
     CDS             15960..16847
                     /locus_tag="VGQ51_14280"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEV9037797.1"
                     /translation="MRKYSLFLIGMTLGHASFCQPTPLTVHPSTTRHGPEHGSLLIIG
                     GNVGSTASIWNTFTELAGGKDKAHIVVVTTASGDSAAYDQKDVDVVKKQTGISDVVLL
                     HTSSLREANSEKFIEPLKRATGVYFVGGRQWRIADSYLNTLTHQAFFDVLNRGGVIAG
                     SSAGASIQGSFLWRGDTRGSEILIGDHTQGLGFLKNSVIDQHLLRRNREFDLVNLIEK
                     APQLIGIGLDEATAILVKGDTLQVIGKSYVAIYDYTTITKSANLSSGPFFFLGEGQKY
                     DLKQRKVIQAPRQGLAEKD"
     gene            complement(16738..17358)
                     /locus_tag="VGQ51_14285"
     CDS             complement(16738..17358)
                     /locus_tag="VGQ51_14285"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV9037798.1"
                     /translation="MIKRFFDLAVSIAVILCILSWLMPVLALLILLDSGGPVFFVQKR
                     VGRQGRPFNCYKLRSMAAGSITRCGGWLRRSHLDELPQFFNVLLGSMSLVGPRPYMPS
                     DCRVFADMVDDPDSRHRVKPGITGMAQSKGLHETSCDPDIVRQRYYWDNWYVENAGWL
                     LDLRILGRTALNLFPRDPAAVPGSPFSASDRISVLPLEKRKAPMRG"
     gene            17433..18359
                     /locus_tag="VGQ51_14290"
     CDS             17433..18359
                     /locus_tag="VGQ51_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037799.1"
                     /translation="MALRSSILKTVAYFDLFSYPLTREDIRYFLDTDAGEEEVDHELT
                     RLTKEGRLYRLGTGTFYSLRNDPELAEKRLRGEVHADELLRIAARGARFLYQFPFVRG
                     VCISGSLSKRCADKNADIDYFIITKANRLWIARTIMHLFKKLTYLRGRQHRYCMNYFI
                     DEEALEIKEKNIFTAIELLTLMPACGNGGLTQFFQANNWTCQYLPHYRDRQREADGPY
                     RSSLLKRGLERLFNNRLGSLLEDYLRRLTDSRWKTKTSRGYKNMKGDPMTLQCGLHFS
                     RPDPGIFQQRILLLYQQKLNEVAVDPVSFSAK"
     gene            complement(18316..19158)
                     /locus_tag="VGQ51_14295"
     CDS             complement(18316..19158)
                     /locus_tag="VGQ51_14295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV9037800.1"
                     /translation="MPSEDLINEKQAAAAFSKQAPVFDGYDATNTIIQYKRQRVREHV
                     MSLLRPESRILELNAGTGGDAVFFARLGHRVHATDIAEGMQVKLKEKVRDLGLQDKVT
                     AELCSFTALAGLRDKGPYDLIFSNFAGLNCTRELDRVLRSLGPLLKPGGVATLVILPK
                     FALWEMLLLFKGRFRTAFRRWRAGRKGAPARVEGVSFRCWYYSPSFVIRTLKDSFDLL
                     HLEGLCTIVPPSYIEHFAERHPAAYGWLCRREDKWKNKWPWRSIGDYYIISLRKKPDR
                     PPLH"
     gene            complement(19151..20608)
                     /locus_tag="VGQ51_14300"
     CDS             complement(19151..20608)
                     /locus_tag="VGQ51_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV9037801.1"
                     /translation="MNSILFSHSYFMRYDPKQWAMGQPYPPLATIGAAALLRQYGYRV
                     SLFDTQLIGSPEELSPRLAAVRPDVFVLYDDGFNYLTKMCLTNMREAAFRMVGLAKQQ
                     GAIVVVSSSDSTDHYQQYLERGADFVLLGEGEQTLLELVNAFRNGIGYTEIAGLAFLQ
                     GQQPMRTAPRKVMKDLDSLPIPAWDLVDIESYRAIWMRHHGYFSLNISTTRGCPFKCN
                     WCAKPIYGNRYNARSPRHVVEEVKWLKTHTGFDHIWMCDDIFGLKPGWVDEFADLIEK
                     EGLHFRFKIQSRADLLLQENYVRALARAGCENSWMGAESGSQKILDAMDKGTTVGQIE
                     AATRLLREHGIRPSFFIQFGYPGETKADIDMTIRMINRLLPDSLGISVSYPLPGTVFY
                     ERVKTDLKEKSNWTDSDELKLMFSNTYCAAFYKQLHRYVHRSYGWHLALRQLGQMALR
                     PARITGGRLRMAASLAWQLPAAIVARIRLKRLEHA"
     gene            complement(20605..21651)
                     /locus_tag="VGQ51_14305"
     CDS             complement(20605..21651)
                     /locus_tag="VGQ51_14305"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV9037802.1"
                     /translation="MHFPPLLHTLRRYRTLQTDRISALPIVILMPHSACNCRCVMCDI
                     WKGNPHLRQLTEKDVEGLMDSLRRLGTRQVLMSGGEALLNRNFFALCDILRREDIRIT
                     LLSTGLLLGRHMDDILEKVSDVIVSLDGDEALHDQIRNTPGAFRKLKEGVAELHSRRP
                     DFRITGRTVIHRWNFRSWPAIIDAARNMGLDQISFLPADVTSEAFNRSDPWDQPRQNE
                     ILPSKEELPLLQEILQEVIRDHAGLFASGFIVESPEKLLKIGQYYTASYGLNPFPFKK
                     CNAPWVSAVVEADGSVRPCFFLPSLGNIREQSLMDILNSPSAVDFRKTLDMEENPTCK
                     RCVCYLNLPPRAVL"
     gene            complement(21657..22424)
                     /locus_tag="VGQ51_14310"
     CDS             complement(21657..22424)
                     /locus_tag="VGQ51_14310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV9037803.1"
                     /translation="MNLPDSSILTNIQVQGHTRLGEMLFEKQYIQVRSLEGRLYTDQE
                     LLHLPDIEPGHPHFREWQTRRRSTRRLLRYLSTRRASMDILEIGCGNGWLSHQLAELP
                     GTRVTGVDINFTELQQAARVFNNDPNLRFIHGDIRSGILEDRTFDVILFAASLQYFPS
                     AKKILHLCLSYLKPGGEIHLLDTPFYRPEEVPLAARRTLAYYTSFGYPEMADYYSHHS
                     FTDIRSFHYTILYNPKDFRNRFFRSKAPFPWIRIRNN"
     gene            complement(22563..>23504)
                     /locus_tag="VGQ51_14315"
     CDS             complement(22563..>23504)
                     /locus_tag="VGQ51_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV9037804.1"
                     /translation="LGDKTLAYHSSIGCPFTCSFCAVVPIYEARWKAKTAEQVYRDIK
                     FIKDNWGADGIEFHDNNFFVSEKRVTEFARLILPEKMNWWGMARIDTMDKWKDESLAL
                     IREAGCRIIFFGAESGNDSVLNQLDKGGTQSGAQILRFAQRLKKYDIIPEYSFVLGVP
                     APTPEKVMEQIDFDINFIKKVKAVNPSTEIVLYTYSPVPTEGSELFEQVKKAGFTYPE
                     KLEDWMTPQWERFDLRKNPLTPWLRPEMIDKIRDFETVLNGYYPTVSDIRLNSLKRKV
                     VRLLSAIRYRTDLYKHPYEIKLLHKIWRYRQPEIQGF"
BASE COUNT         5547 a         6633 c         6254 g         5070 t
ORIGIN      
        1 ggttcgttga aatcatgcgt cagcgttcgg aaagcattgg cattcccttc gaaatatagg
       61 aaatcgcgct gcctgttgag actgagctcg ccctcgaaac gcttttgggc ttctccccgc
      121 agctcctccc ggaaaaagat ccggatcgcc tcctctcccg aatgcccaaa cggtggattg
      181 cctatatcgt gcgccagaca tgcggccccg atgacggaag acagctcata ccggtaaaat
      241 tccaggaact ccccgccggc atcaggatat ttttcactca acgcatctcc taccgcccgg
      301 ccgagcgacc ggcctacgga tgccacttcc aggctatggg taagccggtt atggacaaag
      361 accgacccgg gcagcggaaa gacctgggtc ttgttctgca aacggcggaa ggcggaagaa
      421 aaaatgatcc ggtcgtagtc gcgcaaaaaa gaggtcctcg cctcgctgcc ctcgccaccc
      481 tttcgcctcg gtcctccgcc ggtgcgcacc ggagaataca attgttgcca gttcatgcgg
      541 tctttgctat tgcctgatga cgatcttcag atctgccacc agtttttttg ttatgatatc
      601 cgatcccgca gccgtcaggt gaccagggtc gacaaacagg ctgtctttga acagcgtcgt
      661 atcgtaatag ttcaaataca cacctccata gcgcccgacg atgagacggc aactgtcgaa
      721 atgcgcattc agccattgtc tcccgttccg gccaacgaat gcctgcatcg gtgcctgtgc
      781 aaaaacaagc ttaacccttt cctgttgcaa ccatgcgcac aatgaatcca gttcacggta
      841 ctgatgttcg aaccccgaag catgttcgct aaaatagaca ttcgggtcgg cgcgggcact
      901 gtcgtctccg tgaagggtct ttttccccat ctcgaaaagc gccttccccc tcccccattc
      961 gtcgataccc agatgggaag aattgtattt ccgcaggaga agctggtgga tggaatggta
     1021 atcggaaaaa taaaaatacc cgggaaaatg ttccctgatg aacgggctgg tggctgtgta
     1081 gttgcggtag gtggggttgt cggcctcggt gaactctgca ggagagctgc agatgacaag
     1141 ataatccgga tggtaggtgc ggaccaggtc cattaccacg acccgcgtgt ccccgatctg
     1201 catgctccag gaagcgaaat tataataggg cagatgcaat tgctccacga tcatctccga
     1261 gttcagggca taggccgtca tggaggagcc ggcagccatc agccttacat ccttcagcgg
     1321 atgccgttgc aaccattgca tcttgtgatc gaaaaatccg gagttggtca tcatcggcgg
     1381 gacacccagc cgggacaaga tcccgaagcc gatgatgacg ggcagaagga aaaaaaatgt
     1441 cttgagcaaa aacctggtca ttcagctgca tttagaccta gaaccgaaag taaatgaaat
     1501 cggcattttt tgttgcggcg aaggtaagga tgatccccat caaaccgaaa taaatgagcg
     1561 ctctcacggg gaaggcgcgg atcccatcga tctgcaggac atgcggcttg ccccgttgcg
     1621 cccattcggc gacaaacatc cccaaaatgc agaccagcgt aaccacggtg ttcacctttt
     1681 cgaagatgaa gaacggcgaa aaaagcgaag tctggaaaat cctccggtag aatcccaggg
     1741 cctggtccag gctgacagac cggaaaagga tgatcgagac catgaccagc aggaatgtcg
     1801 cgatccgccc gattcccgct gccctcctcc ggcgaaagcc acgtaaaagc atcggtatga
     1861 aataacagcc gttcagcagt ccgaagaaga catatgtcca gctcggtcca tgccaaagac
     1921 cgataagcgt gaaattgatg agaacggcga ggatcaggcc acccttgcca agatcgcgaa
     1981 gaaagatgtt cagcggggta aaaacataat ccgtcatcca gctggtgagc gtaatatgcc
     2041 atctgcgcca aaattcggcg atgttcgtcg ccaaaaaggg ataattgaaa ttcgggctca
     2101 ccgtaaaacc cagtagcctg gacataccga tcgccatgtc cgaataccct gaaaaatcgg
     2161 catacaccca gatagtggcc ataaacgcgc catagaccaa agcgctggca ggcaaactgg
     2221 cataatgagc gaaaatgtcc gatgagaacg acgcgcaatt gtcggcaatg accacctttt
     2281 tgaaaatgcc ccataggatc tgtcgcatcc cgtcggagcc ggcgctatac cggaattcac
     2341 gtcgcctttc cagctgcggg atcagcgatg cgggccggtc gatcggtccg gccagcaaac
     2401 acggaaaaaa tgcgatatag gaacaaaata cgacgacatc gccggccggc gccgtctttc
     2461 ccctccttat atccaccagg tagctgatca tcttgaaggt ataaaagctg agtccaaggg
     2521 ggagcagcga atggaaatat ttaaaataca gaaggcttcc gaggctgata agcaggccga
     2581 cgcccagcag gagatttttt cgaccttcgc gggcggtccg gcacagccac gcgcccaaaa
     2641 aatacaccgc cagggaagtt ccggccagca ggaagagaaa atgccagtcc caccatccat
     2701 agaagaaata actgccggca aggatcaacc cgttttgtgc ctttatcgta ccggcgaaaa
     2761 cgaaccagta caggataaag aaagcaacaa aaaagagaaa gaagggaagc gtggtaaaaa
     2821 gcatgtcaat ccttatttgc gctgagtaat tcgacgaggc cataggacgc ctgcagaaaa
     2881 gctactttcc tgcccccgaa caatacggac ggtttgggag gtgaaaggag cagggcgttt
     2941 tgcaggacca gccgcgcgat ctccgattct atatcaccca cttcaaaaca gagatggtaa
     3001 tagccgatgc ccttccgaag aagtgtttgc acgggcgcac cggcaacgag ctcgatacgg
     3061 gtgccgtcag ccagcgtcag cattgcgacc gtggcttcct ggcagggatc gtatacgatg
     3121 ggagtgcgtt ccaggacctc atggaccctg caaatattcc cgatctcttc ttctatgtcg
     3181 cggcaagcga cgcctatatg atgcagctgc ataactttta tcctaatttg gtcgagagcg
     3241 tatccaccag ctgcccgaca ttcttccatg actggatctc acgcgaggtg aaccgtactc
     3301 cgaatttctt ttcgatagcc accactagct ggatatgcgt gagggaatcc cattccgcta
     3361 cgtcgccggc ggtcgtttcc ggggtaagta cgatcgaccg gttctccagc acttcgatga
     3421 agattgaatt gatggtttcc aggatagtct tcttatccat gcttatcgga ttgtttggtt
     3481 aaaaaagcac catatagaac ggctaccttc agctgttcga ccttttccca ggacacgtct
     3541 tcggccggca aagccttttc cgacccgatg ctgaaacctg ccgaccgcac catccggcgt
     3601 atctcttcca cgttgtggaa aagatacaga tgatcgatag cgggcgcatt cgccggtgtg
     3661 gtaaaaaaca aaacgccatc atcggcaagc agttctttca gacgcaggag cagggccagc
     3721 ggctgctcta cgtgctcgag gacctccccc aacacgatca gatcgtattt ttgctccgct
     3781 tcgaatgtaa aaatgtcgtg cagccggtag tcgatacggt cttcaccggc aaatttccgt
     3841 gacagggcga tcgacgtctc gctgatatcc acggcggtaa ggctggcccc ggaagagagg
     3901 gcttcgcggg cgtgagacag gtaaaaccca agtcccaccc ctacttcgag gtaatggagg
     3961 acgccgccgg tataacgggg aagtaccgtg gaataatagt cgaacatctc gtagtgatgc
     4021 cgccacagga attgcgataa aatgagcccg tgcatataat attgcatcgt ctccggtctg
     4081 ccatagacgc gtttattcac ctcttcgaag gtgctgctgg tatatttgcc ggttcgcgcg
     4141 aattcgatcg tttcggtcat cacgtcctcc accatgcgca gatagcaatc gacggcatag
     4201 tcaatgtgtt tcccttcccg gttgaggatc gcctcgtatt tttgaaagaa agcttctgcc
     4261 agccggtggt accggtcatc ctgcaccgcc aggtttttca tcagccgctt cccatgcagg
     4321 ggatcctgtg cggcgatctt tgcggttatg gtatcgacga tgcgtgtcat aatgcgtttt
     4381 aggatgctga ccggatctcg gttttcaggg tctggaaggt cccggtatcc aaaagccagt
     4441 acgcgtcccc gggctgaaat ccgagctttt cgtaatggcc ccttaccagc tgattctttg
     4501 gcgtgggcag gtactctcct ttcaggaacc gaaatccccg cttccgcgca aaatcaacga
     4561 ttgaatttaa ggtgaactgt tccattcccc gttgcaatac ccggcagctc atcaaccagg
     4621 tatcgatgaa caaggtttcg tcggataccg tctccaggat gaccaggcag acaatgccat
     4681 aggctccgag acggtcgtcc agcgaaaaag cgaatgtgaa ccggtccggt tccccggcca
     4741 tacgcagaat atccatttcc gtataacgaa cggttcgcag gttgaactgg ttgctccgct
     4801 ggcacaattg cgccacccgg ggactattga aggtgttgaa gggctcccag cgcgatacca
     4861 tttccagtcc cgacaggaag gcctcctctc ccgcatagtc tcgcctttcg acggcacgaa
     4921 gcgcttcagt ctgataaagt tttgtccttt cggcatcctc gtccgatctg gatgaagtgt
     4981 cgaagagatt tagaccatag aggaattcca gccagtcggc aggatcttca ggcaattcgg
     5041 ggacggtgat gtctggtatc gccgcccgga ccatctccct ttcaaagggg ctgtcgtcca
     5101 ggaataccat ggaatcgaaa ccgatgttca ggacagcctg tatatcccgg agattgtcga
     5161 ctttgctgtt ccagttgacc cggaatattg cgatgtcatc gagccccagc accatatccg
     5221 ggtgctcccg gaaaggttga agtgcgatgg actccgtatt cttgctgcat accgccagga
     5281 tgatgccccg ccgggccaat ttttttatcc agtactgcaa cgccgagaaa gctttgccga
     5341 tacccagcga tccgatctcg atatgttcca ttccatcgtc gccgataacc ccgccccaga
     5401 gggtattgtc gagatcgagc acgagacatt ttttggatcg gccgaggagc gcaccgatac
     5461 aagcggctgt cgcggccacc accgcaggga gggcctccga gactgccata gcggcgtgga
     5521 tatacagcat cgggcggtac agtgccggtc tgccgatcct attctggatg gtggccaggt
     5581 ctacgatata aaaacccggc tgcccggcag cgtaggtcat tagtccataa ttgagcttac
     5641 gcaattggaa aaggaaagat cgctctgttt tgttagaatg atttccgaat acggcatcgt
     5701 ccgtttccgg ataattataa tagatgaggc tggccttaca ccgtgcatgg acagccgact
     5761 tcaattcatc gaccagcctc agacggtcgt cggccagctg atcctgctca ccggggctca
     5821 accggtcgta tatttccagc agtctttgag tggaatgaaa gaggatgatc acctgcgggt
     5881 cacggcggta tagctccgaa tccggatcta agacctctct ttcgatctgg tcgaaaccgg
     5941 cttctgtcac cgacagaaga agcccgcacc cggctcctgc gctgctcagt aatctggcga
     6001 gtagctgtgt ggaagtatcc cccagcaaaa cgacgcggac ggcgctgacc tcgcacatat
     6061 aatccttttc gtttatttta ttggcctgga cccgatcctg ttcaccagcg tgccgatgcc
     6121 gtcgatgccg atctctatgc tgtccccttc ctgaagcgtg aattcctgag aagggacaag
     6181 acatgtccct gtcatcagga aacatccgta ttcgaaatcc atttcgcggt atagccactg
     6241 cgccagttcg ggcagccggc gtttgatctg agagatcgcc acttccccgg aatagacgct
     6301 ggccccgttg cgccggatga ccatgctgat ccgggtcgaa gaaggcagag gttcggcggg
     6361 gaccagtaaa catggtccca gggcagcgct gcggtcatag acttttgcct gcggcagata
     6421 gaggggattc tcaccttcga tgctacgcga gctcatgtcg ttgccgatgg tatatccctg
     6481 gatctcgcca ttcgaattga taaaaagtgt caactcgggc tccggcacat cccaggagga
     6541 atcacgccgt atatagacat cctcacaatg tgcggccacg cgatgcggta aggccttgaa
     6601 aaaaagttcg gggcgctcgg cgacatacac tttatcataa aaggtcgctc cgcccgactc
     6661 cgcggattct tccatacggg catcacggct gcgcagatag gtgacgccgg cggcccacac
     6721 ttcctgccct gtcccgatgg gggccatcag ctgccccttc aacaaggcat ccgcttccgc
     6781 cgcaggaagg gtagcggcgc cgctcaacag ggatcgcaga taacgatgca gctcactccg
     6841 gttgatcagt tgttcccagg gctgatccag caaccggtag tcctgtccgt cgtttacgag
     6901 ggtgcctctc cgtgtacgat ataacttcat aagggccaaa tatagcatag taataggcga
     6961 ccataaaaaa ttaagccgat tttaatggct ttctcttcct atattgagtt taattttgta
     7021 tattaagttt tccgacccga cacctaacct gcctgaaacc ttgagaccac atgaaagaaa
     7081 cagcgtccga caccgttgaa ccgcattctt tttctttgcg gaacctatcc attcgcaaac
     7141 agcttcccct gctgatctgt cttttattac tcatcctcat tcttttattc ggaacgatct
     7201 cctatattag tatccggcgc gcaacaatgg ctatcggtca acaacgtgtg cgcagtctga
     7261 ccgaacagct cgcctccctg ttccagcaat cggcgcatac cctcgcatcg ggggcgcaaa
     7321 gggccgccga caaacccgaa atcgtccgct tcctatctat acaatacgac aaaggggcct
     7381 cccccaaccc cggcgagaca ttgcctaccc tgcaacggct gctggcggac tcacagaccg
     7441 tgctggcgga attacgcgat ctgcagggaa atatcctgct acgggcagga cgacagatcg
     7501 ggtcctccgg agaaacgggt agcccctccg gtcccttcgc cgcaggtgag gtcgccggca
     7561 ggaccgacag caattttgtc ggtaaattct atttcagcga agacagcata tattaccccg
     7621 tcatagccac ggtcaggcgc gatggcagat ccatcggcta tctctaccgg tggcggccgc
     7681 tgcgcgcgac ccccaaatcg cttgcccagc tgtcgcagtt gatgggctcc aaagccgcgc
     7741 tttatttcgg caatcacgac ggctcgcttt ggacggatat gctcaaaccc gtcgctgcac
     7801 cgccgcaggt gccgaatgac tcgcatagga tcgtcacata cacaagaccg ggaggcaggg
     7861 taattgcaga tgccacgccc gtggccggca ccaaatggat cgtcctggta gagctgtcga
     7921 gcgatctgat catggaggcc tccaaccgat ttcttttctg gatgatcctc atcggcgtgg
     7981 tgctcatcct gacgggtagc tttatcgcct ggttgatcag ccggaatttc acccggccgc
     8041 tggaccggct catacacgcc accaccgcca tcgccgccgg cacttattct cccatcgtcg
     8101 tcgcagacag acgcgacgag atcggcaagc tggccaggtc tttcgattcc atggctgtgc
     8161 aggtgcggca tgcgcacgac gaactggaaa acaaggtcag catccggacg aaggagctgg
     8221 aagtggtcaa taaagaactg gaggcttttt cttattccgt atcccatgac ctgcgtgctc
     8281 cgctgagggc cgtcagcggc tatgccatga tgctgaagga agattatgag gaccaattcg
     8341 acgacgaggc caaacggatc accggcaata tcctcagcaa tgtgaagatg atgggcaggc
     8401 tgatcgacga cctgatcgcc ttttcccggc tgggcaaacg cgaggtccgt agaaagatca
     8461 ccgatatgca ggcgctgaca gagacttgcg tggccgagtt gaaacagggc tggcccgagg
     8521 ataaattcct gatctcggtg gacccgctgc ctccctgcaa aggcgatgaa gatctcctca
     8581 aacaggtatg gatgaacctt atcggcaacg ccatgaagta ttcggccaaa aatcccgatc
     8641 cgcggatcac catcggggcc acccacaacg gggatgccgt ccatgacatc tatttcgtcc
     8701 gcgacaacgg cgcgggtttc gatatgaaat atgccgacaa gctgttcaag gtctttcaac
     8761 gcctgcacag ccaggaggaa ttcgaaggca caggcgtcgg gctggccctg gtcaaacgga
     8821 tcctggataa acaccagggt gagatttggg ccgaatcgaa gcccggtgaa ggaacggtat
     8881 tctatttcaa attataaaac gccaaacctt atgcttaacc acgtcgaggt attattagta
     8941 gaagacaata tccatgatgc ggaaatgacc atccgggctt taaaaaaggt gaacctggcc
     9001 aaccatctcg tacatgtaaa agatggagcg gaagccctcg atttcatctt tgccaggggg
     9061 aatttctccg gtcgcgattc ggaaggcctg cccaaagtga tccttctcga tatcaaaatg
     9121 ccgaaggtgg atggcatcga agtccttcgc cagctgaaat catcggagct cagccagacc
     9181 atacccgtcg tgatcatgac atcctcgaaa gaagaacagg acattatcag cagctacaag
     9241 cttggcgtta acagctatgt ggtcaagccc gtcgatttcg aagggttcgc gagggccgta
     9301 agccaactgg ggctgtattg gctgctgacc aatcaaccac ctatgaatga taatcaacca
     9361 taaatccatt gaacatggaa actttaaaaa tattgcatct cgaagatctg cccgccgacg
     9421 ccgaaatggt cgagcgggtg ctgcgcaaag cgaatttccg cttcgaaaag aaagtggtcg
     9481 tggacagacc cgattttgta cgcgcgcttc acgaattccg accggatatt atcctctcgg
     9541 accactcgct gcccgccttc aattcgctgg aggcgctgcg catcacccgg gaggaaggca
     9601 ttagcgcacc gctgatcctg gtcaccgcca cggtctccga agagtatgcc gtcaatgtta
     9661 tcaaggaagg cgccagcgac tatatcctga aggatagact cgaacgcctc cccaatgccg
     9721 tccagaacgc attggacaaa tgcctgatgg aaagacaacg ccggcaggca gacgaggccc
     9781 tccgcgcttc cgaacggaaa tacaagctgc ttttcgaagg caatcctatg cccatgtgga
     9841 tgttctcgct gaaaagcatg cggatcatcg acgtcaacga ggccgccaca gaacattatg
     9901 gctatacaaa ggaagaattc atgtccgtgg ggacgagtca gatctggcag ggcgccaacc
     9961 cgggcgttta cccatcgggt atctggaaac acaaaaaaaa ggacggcacg atcatcgatg
    10021 tcgaagtcat tacgcacgac ctgcttcagg gacaggagcc ggtgctcctg atattggcaa
    10081 tcgatatcac cgacaggtta agggcggagg tcgaactgtc gaggcaacgg gacacccagc
    10141 agaagctgat cacgcagacc agcatccagg tacaggaaaa ggaaagagag cagatcggca
    10201 aggaacttca tgacaatatc aaccagatac tcgcagctac caagctctac ctggacatcg
    10261 cgatgaaaga aagcgaagaa gagcaggtga ccgaaatgtt gctcaagagc cacaagaacc
    10321 ttcagtccgc catggaagag atccggcagc tgtcgcaatc gctggttgcc ccttcgcttg
    10381 gcggcgtcac cctcgacctc gcgttggcca ggctgatcga aaatctgcgg ctggcctctc
    10441 cgcttaacct gtccctcgat acgtccggtt ataggggaga cgtgtccgac gaagatatga
    10501 agctcacctg ctaccgtatc gtacaggtgc aattgaataa catcattaaa cacgcccatg
    10561 caaaagatgc cgtcattcag ctgaaaaaag gagatatgct ggagctgacc atccgtgata
    10621 atggtattgg cttcagcccc ggaaaaaata cggccggcat cggtatcagg aatatccgca
    10681 acagagtggg cttttataac ggcgaggtcg ccgttctgtc cgagccggga aagggctgta
    10741 cgttaaaggt aactatccct atccccccga cgagccccgg atgatcaata cccagccctt
    10801 cgctttctct accgggatgg gttcatccgg cgcaaaacgc cgtgcgggct gaaaattggt
    10861 gatggccggc gcttcgatcg tcgcatgcgc cggatcgatc cccagacgct gccagtcgat
    10921 gttcagccgt atcacggtat cttccggcgc ccaggaagcc agcgctatca gggcagctcc
    10981 ttttttacgg tagaccgtga cagggatgtc ccctcggccg gtattcaccg ggcaggacgg
    11041 actccaatag ccgatcatct ccgtgccctg catcccgaac tcatcccagg ctttccagat
    11101 cgggcgcggg tccgcattgt ccgaccaggg catcctgtta gtcatgccat agaccattcc
    11161 cctccaggga ttgccgccgc cctgcagcat ctcacccatc aatccgaacg ggataccgct
    11221 cacttccgta aggaagaaat cgggcgggtt atgctcataa tcgaaatatt cgccgaacca
    11281 caggcggttg agatagggaa aatgctccat ataaagattg gcgctattgt tgtatccgtc
    11341 gcgcggattg tactgatttg cggagtgcag atcgatgatc ccgggatggc cgtcttctgt
    11401 gagcacacgc ttgatacgtt tcatcgtgac acgatcgaac gcgacatcgt cgagatagat
    11461 accatcgatg cctacatttt tcaccagcca gttcatgccc tccacataat aattatgcca
    11521 gcggctcatc ccggaattta ttatcgcggc gtcctttatc tcagggacga accaggccgg
    11581 gatatagtcg tcctccacat gctcctgcag ccaggaaaaa cccttgccgg gtccggccga
    11641 atagatctcg tgcccgagac tgcgcagcgc gaacagctca taggcatgat ccgacaattc
    11701 gcgtacggta ttatatatct ttactttcaa ccccaacgcg tgcgccgtgt cgatataccc
    11761 tttcatcctg cgccattcga taaaaggata attgatccac gggttgatgg gtgtggcgtg
    11821 atggatattg atcacggtgg cgcccgtagc cgctacccgc ttcgggtcgt catatttgtg
    11881 ataaaaacgc atcgcccact gcgcatcggt aatcagcgga tggaaaggcg tgatcagcag
    11941 attgaaatta taatagaggg tgtcgccttt tttcatcaga cgatggccgc tgtaattgtt
    12001 ggccagcatc gatttcccct tgacgccgat gtcgataccg ccgttgccgc cattgtccca
    12061 cgacgaggga tcgatcaggg gcttttgcag atagaaattg gtattcagag ggcggacgta
    12121 gcgttcgtcg cgcagagaaa attgcaaacc cgcattgacc gttccgatcc atgcgccgtc
    12181 ctggttctta tgtgcaacat cccacttcca atgatacata ctgtccggcc ggtagccgcc
    12241 tttgcgtccc agacccatca tataggtcgt cgcagccttt tcgaaaggga tgtgcatcgt
    12301 gatatccttt agctcgaggt cctgcaacgc cgtgaccctg acggtatagg caaggaagcc
    12361 gtcgaattcg agcgaggcgc tgacgtccat tcttacttca tccgaggtgc tggtggcctc
    12421 ccagcgcacc gtaccgggcg ttctttccgt gaaatgcaca ccgggaccct tcagccggat
    12481 gtcctttccg tcggaagctc gcgtaaaatg aaagtgtatg ttctccgcga ggaggctatt
    12541 cggccgggtg ccgatggatg tcatttcggg cgtgaaaaag gtgcgtatct gttcggggaa
    12601 accctgtgcc gtaagcctga tgtcgcgtcc caacaggccg atcagcgtgt cgccggagat
    12661 cgtcagcgga gtatagggtg cgatcacggt gttctcctgg gcaagagtgg agtttagcca
    12721 tttcagccgc gtcatcttcg ccggggttgc cgccccgccg tcgctcacct gctgctcccc
    12781 tacccgcagg gtcacctgcc ccttccattg ggcaccgcct gcgataacag tcacgacccc
    12841 tctataaacg ccggaagcga tatcccgcgg aatatcgacc ccgcaccaca gggcttgtac
    12901 ctgtccggcg cttacggaga cgtctttgct gaacggcttt ccgtcattac ttatccctcc
    12961 ggtattgata cagctgattt tatccagcat aacgccgcca gggcctgtca gcgcactgaa
    13021 attcacccgc acgttctgaa tatccttcaa tgcataaacg cccagctgcc acgcaaacca
    13081 ttccccgcgc gaggcgttgc ccgatacggc attgcccggg cctttagcga tccagcggta
    13141 aggcagatca tcgcgcatcc ggatggaatg ttcgcggtct tccgggaaaa caagaaaggt
    13201 cttgccggca tatttctgtt tcaacgcagc cgtctctttc gccgtcgcga tcacctccat
    13261 cggataccag gtattgaaag agtcgatgct ctggaatgca gtaaccgtac agttgacggg
    13321 accggtgcga ttcaatgcgg cgagccattg cggtgcagcc gtcgtatccg gacgccaata
    13381 aacccccttc ggataattgc tacgtccttc gttacgataa ggcaaataat aaacgtaata
    13441 aagcccctta cccgaaaccg gctcgaaaaa cacgcccccc gattcacgcg taaggacacc
    13501 cgtagtcgca ttcctaaccc gcgcgccggt gaccgcatcc tgtacgatga tccgatgagc
    13561 cgcgggatcg gcgacccgaa gacgccaggg cagattgaca tgggcgacgg cgccgggacc
    13621 gcgaaagtcg atagcggcgc gatggttgcc cagcgaatcg ggattccagc agccgggaca
    13681 ggaagtatac ggaatggact gcgcccgcgc gaccagggaa acggacgtcg acaacgcgat
    13741 aaacaacaag gctttaagca tgtgtcgaag ataagccatc ggccctaata tttcccgtag
    13801 tcccgccaaa caaccccgat ttgggttata tttgccgtta cgtcaataaa ctatcgtaca
    13861 tgtcacccat cgcgatctac catgaacatc ccgattggtt ccggcccctg ttcaacgagc
    13921 tggacaatcg cggaattccg tacacgcgtc tgaatccggc agcccaccag ttcgccatcg
    13981 aggcacccgc tccggattac gcattatttt tcaacagaat gagcccttct gcctatctgc
    14041 gggaaggaac gcaggggatc ttttatacgc tcaactatct gaaatatctc gaataccacc
    14101 aggtacccgt cgtcaatggc cacaaggcat ggacattcga gacgtcgaaa gccatgcagc
    14161 tgatgctgat gcaatcgctc ggcataccct atcccagggc ccatgtcgtc aatcaccctt
    14221 tgcagctgat agccgccgcg gaaggactgc gtttccccgt ggtggtgaaa gcgaacatcg
    14281 gcggcagcgg tgcgggcatc accaaatatc aaacattgga agacctgcgg ggcgcagctg
    14341 ccggtgaaat gaactttggc gtcgaccata ccgcactgtt gcaggaattc atccctgcgc
    14401 ggggcggcta tatcacacgc gtggaaacgc tcggcggcaa attcttatat gccatacgtg
    14461 ttcatctcac gggcgacacg ttcaatctct gccccgcgga tatttgtcag acgagtacag
    14521 gggtcgagct ggtgcggaat gcctgcgcgc tcgaggcccc gaagagcgga ctgaaggtgg
    14581 aaggctatac cccgccatcc accgtcatcc atgcggtgga aacgcttgtc cagcactcgg
    14641 gcatcgacgt aggcggcata gaatacatcg tcgacgaccg ggacggagca ctgctgtact
    14701 atgatgtcaa tgcgctctcg aatttcgtgg cggatgccgt caatgtcatc gggttcaatc
    14761 cgcacgaaaa gctggtcgat tacctcgaaa aacgcgcggg tatccgtaag ggcgatgctt
    14821 tcgcgaaaaa aaatgcgcat cgccctcacg ctaatttttg acctgcggtc gggcggcttc
    14881 cagccgcctg ggctcggcgc agatgtctca cgcataaata ccattcgaat gagatacgga
    14941 tactggatgc ccgtttttgg cggctggctg agaaacgtgg acgatgaaaa catgcaaccc
    15001 acctgggact atgtgaaaaa gctggcgatc cgcagcgaag agatcggctt cgatctttcg
    15061 ctcattgcag agctgaacct caacgatatc aagggtgaac atgcgccatc gctggatgcc
    15121 tggtcgacga cggcggcgct ggctgccgtc acccgcaaac aggagctcat ggtcgccgtg
    15181 cgacccacct ttcacaatcc cgccctgctg gccaaacagg cggcgaatat cgaccatatc
    15241 agcaacggca ggctatcgct caatgtcgtc tcctcgtggt ggcgcgacga ggccaccaaa
    15301 tacggtgtgg gcttcgagca gcatgacgat cgttatgcgc gtacggccga atggctcacc
    15361 gttctcaacg gcgtttggaa ccaggacggt ttcacctaca aggggaaatt ttaccaggtg
    15421 accgacaata cgctgcagcc taaaccggta tcgagaccga gaccgcagat ctatgccggc
    15481 ggagaatccg aggcggcaaa agacctgatc tcgtccacct gcgacggata tgtcatgcat
    15541 ggcgacgagc ccgccaccat cggcgcgcgg atcagggatc tgaaggaaag aagggagaaa
    15601 aaggggctgc cgcagatgcg atatggcgtg gccgcctaca gcatcatccg gaacagcgac
    15661 gccgaagtca aacgcgagct cgccaggatc accgatgtcc gtccgggcag cccggggtat
    15721 cacaattatc agcaatggct ggcgggtacg cagttagaac aaaaggtgtc gctggaagat
    15781 tattcggtgt ccaacagggg actccgttcg ggcctcatcg gctcgccggc gcaggtgcag
    15841 gaccggattg gtgaattcgc cgatcagggc gtggatttct tcctgctgca atgcagcccg
    15901 caactggagg agatggagcg ctttgcagac gcgatcataa cggagcaata cgcaaaagca
    15961 tgagaaaata cagcctcttt ctgatcggca tgaccctggg tcatgcttct ttttgtcagc
    16021 ccactccgct gaccgtacat ccttctacta cgcgtcatgg tcccgaacac ggatcgctgc
    16081 tcatcatcgg cggcaatgtc ggttcgacgg cctccatctg gaataccttc accgaactgg
    16141 cgggtgggaa agacaaggcg cacatcgtgg tggtgaccac cgcctccggc gacagtgcgg
    16201 cttatgatca gaaagacgtc gatgtggtga agaaacagac gggcatctcc gatgtcgtgc
    16261 tattacatac gtcctcgctg cgcgaagcca attccgagaa attcatcgag ccgctgaaac
    16321 gcgcgacagg tgtctatttt gtgggtggac ggcaatggcg gatagccgat agttatctga
    16381 atacgctgac gcaccaggcc tttttcgacg tgctcaaccg gggcggcgtc atcgccggca
    16441 gctcggccgg cgcctccata cagggctcat ttctctggcg cggtgatacg agaggttcgg
    16501 agatcctcat cggagatcat acccagggtc tcgggtttct gaagaattcg gtcatcgacc
    16561 agcacctgct gagacgcaac cgggaattcg atctcgtcaa tctcatcgag aaggcgccac
    16621 agcttatcgg catcggtctt gacgaagcca cagcgatcct cgtaaaaggc gataccctac
    16681 aggtcatcgg caagtcctac gtagcgatct acgactatac gacgatcacg aaaagcgcta
    16741 acctctcatc ggggcctttc ttttttctag gggaaggaca gaaatacgat ctgaagcaga
    16801 gaaaggtgat ccaggcaccg cggcagggtc tcgcggaaaa agattgagcg cggtacgacc
    16861 caggatacga agatccagca gccatcccgc attttcgaca taccagttat cccaataata
    16921 cctctggcgt acgatgtccg gatcgcagga ggtctcgtgc agtcctttgg attgtgccat
    16981 tcccgttatt ccgggtttga cgcgatggcg gctatccgga tcgtcgacca tgtccgcaaa
    17041 gacgcggcaa tcggaaggca tataaggccg gggaccgacc aggctcatag accctaataa
    17101 tacattgaag aattggggca gctcatcgag atgcgagcgc cggagccagc ctccgcaacg
    17161 cgtgatactt ccggcggcca tgcttctcag cttgtagcaa ttgaatgggc gaccctgtct
    17221 gcccactctt ttctgaacga aaaaaacagg tccgcccgaa tccaggagga tcagcaaggc
    17281 cagcaccggc atcagccagc tcaggataca gaggataacc gcgatcgaca ccgccaggtc
    17341 gaaaaaccgt ttgatcatat atgaaatgaa cgcagcctgc gttccgccgg ttgccccggc
    17401 aaccgatgtc ctccccattg cgttctataa ttatggcact tcgatcgagc atcctgaaaa
    17461 cagtagcgta tttcgatctg ttcagctacc ctttgactcg cgaggacatt cgctatttcc
    17521 tcgacacaga tgcaggggag gaagaggtgg accatgagct gacccgcctc acaaaagaag
    17581 gccgtctcta ccgtctggga accgggacct tttactcgct gcgaaacgac ccggagctgg
    17641 cggaaaaaag acttcgcgga gaggtgcacg cggacgaact gttgcgcata gccgcccgcg
    17701 gtgcgcgttt tctttaccag ttcccgttcg tacggggcgt ctgcatctcc ggctctctct
    17761 ccaaacgttg tgccgataag aacgccgaca tcgactattt catcatcacg aaggccaacc
    17821 gtctctggat cgcgcggacc atcatgcatc tctttaaaaa gctgacctat ctccgcggac
    17881 gccagcatcg ctattgcatg aactatttta tcgacgagga agccctggaa ataaaggaaa
    17941 agaacatatt caccgccatc gagctcctca cgctgatgcc cgcctgtggc aacggcggcc
    18001 tcacccaatt cttccaggcc aacaactgga cctgccagta tctcccgcat taccgggacc
    18061 gtcagcggga ggccgatggc ccgtaccgat cctccctgct gaaacgtggt ctcgaaagat
    18121 tattcaacaa ccgcctgggg tcccttctcg aagattatct tcgccggctt acggatagcc
    18181 ggtggaaaac caagacttca aggggttaca aaaacatgaa gggcgatccg atgaccctcc
    18241 agtgcggact ccattttagt cgtcccgacc ccggcatctt ccagcaacgg atcctgctgc
    18301 tataccagca aaagctcaat gaagtggcgg tcgatccggt ttctttctca gcgaaataat
    18361 ataataatcc ccgatcgatc tccagggcca tttgtttttc catttgtctt cacgccggca
    18421 caaccagccg tatgctgccg gatgacgttc ggcaaaatgt tcgatatacg atggtggcac
    18481 gatggtacaa agcccttcca ggtgcaggag atcgaacgag tcctttagcg tgcggatcac
    18541 gaaggaaggt gaataatacc agcaccggaa actgacccct tccacgcgcg ccggagcgcc
    18601 tttgcgaccg gccctccaac ggcgaaaggc tgttcggaac ctgcccttga acaagagcag
    18661 catttcccag agcgcgaatt tcggcagtat caccagcgtc gctaccccgc ccggttttaa
    18721 cagggggccg agcgagcgca ggacccggtc gagctcccgc gtgcagttca gccccgcgaa
    18781 attggaaaag atgaggtcat aagggccctt atcccggagc ccggccagcg cggtgaacga
    18841 gcaaagctcg gccgtcactt tatcctgcaa accgaggtct ctcacttttt ccttcagctt
    18901 cacctgcatt ccttccgcta tatcggtggc atgcacgcga tgaccaagac gcgcaaagaa
    18961 taccgcatcc ccgccggtgc cggcattcag ctcgagaata cggctttcgg gccgcagcag
    19021 gctcataaca tgctcccgca cccgctgacg tttgtattgg atgatggtat tggtcgcatc
    19081 gtacccgtcg aataccggcg cctgtttgct gaaggcggca gccgcttgtt tttcatttat
    19141 caggtcttcg ctaggcatgt tccagtcttt ttagtcggat ccgggccacg atggctgccg
    19201 gtagctgcca ggccaatgat gccgccatcc tgagacgccc ccccgtgatc cgcgcgggtc
    19261 gcagggccat ctggcccagc tggcgcaagg cgagatgcca gccatagctc cgatggacat
    19321 accggtgcaa ttgcttgtaa aaggcagcgc aataggtatt gctgaacatc agcttcagct
    19381 cgtcggagtc cgtccagttg cttttctctt tcaggtcggt tttgaccctt tcataaaaaa
    19441 cggttcccgg caacggatac gaaaccgata tcccgaggct atcgggcagc agtcggttga
    19501 tcatccggat ggtcatgtcg atatcggctt tcgtctcccc gggatagcca aactgtataa
    19561 agaacgacgg ccggatgcca tgctcccgca aaagacgcgt cgcagcctct atctggccga
    19621 cagtcgtccc cttgtccatc gcgtcgagta tcttttgcga tccgctctcc gcgcccatcc
    19681 agctgttctc gcagccggca cgcgccagcg cccgtacgta attctcctgt agcagcaggt
    19741 ctgcccgcga ctgtatcttg aaccggaaat gcagcccctc cttttcgatc agatcggcaa
    19801 attcgtcgac ccagcccggt ttgagtccga agatatcgtc gcacatccag atatgatcga
    19861 aaccggtatg cgtcttcaac catttgacct cctcgacgac gtggcgcggg gaacgggcat
    19921 tgtaacgatt gccatagatg ggtttggcgc accagttgca tttaaacggg cagccgcgcg
    19981 tagtgctgat gttcagggaa aaatatccgt gatgacgcat ccagatggcg cgatagcttt
    20041 ctatatcgac caggtcccag gccggaatag gcaggctgtc gaggtctttc atcactttcc
    20101 ggggcgccgt cctcatcggc tgctgaccct gcaggaaggc aagccccgca atctccgtat
    20161 atccgatccc gttgcgaaag gcgttgacca gctccagcaa tgtctgctcg ccttctccaa
    20221 ggagtacgaa atccgcgccc ctttcgagat attgctggta gtgatccgtg gagtccgaac
    20281 tcgagaccac tacgatggca ccctgctgtt tcgccaggcc gaccatccgg aatgccgctt
    20341 cgcgcatatt ggtcagacac attttggtca ggtagttgaa tccgtcgtca tacaggacga
    20401 agacatcggg ccgtacggcc gccagacgcg gtgacagctc ttcggggctc ccgatcagtt
    20461 gcgtatcgaa cagcgagacc ctgtatccat actgccgcag aagcgcagcg gcgccaatgg
    20521 tcgccagcgg cggataaggc tgacccatcg cccattgctt agggtcatac cgcatgaaat
    20581 acgaatggga aaaaaggatg ctgttcataa tacggctctt ggtggaagat taagataaca
    20641 cacacaacgc ttgcaggtcg ggttctcctc catgtccagc gtcttccgga aatcgacggc
    20701 cgaggggctg ttgaggatat ccatcagcga ctgctcgcgg atattgccca aagacggaag
    20761 gaaaaagcag ggacgcaccg agccgtcggc ctccacgacc gccgacaccc agggcgcatt
    20821 gcatttttta aaggggaaag gattcaggcc ataggaggcc gtatagtact gcccgatctt
    20881 aagcaacttt tccggcgact ccacgatgaa cccgcttgcg aatagcccgg catgatcccg
    20941 gatgacttct tgcaggatct cctgcaacag aggcaactct tccttcgaag gcagtatctc
    21001 gttctgccgg ggctgatccc agggatcgct gcggttgaag gcttcgctcg tgacgtcggc
    21061 gggcaaaaag ctgatctggt ccagacccat attacgggct gcatcgatga tggcaggcca
    21121 ggacctgaag ttccaccggt ggatgaccgt acgtcccgtg atccggaaat ccggccgccg
    21181 ggagtgaagt tccgcgaccc cttctttcaa cttccggaat gcgccgggtg tgttgcggat
    21241 ctggtcatga agggcctcgt cgccgtcaag cgagacgatg acgtcgctta ctttttccaa
    21301 aatatcgtcc atatgccttc ccaaaagaag cccggtggac aataaggtga tcctgatatc
    21361 ctcgcgtctc aggatatcgc aaagagcgaa gaagttgcgg ttgagaagcg cctcgccgcc
    21421 ggacatcaat acctggcggg tcccaagacg gcgcagactg tccatcagcc cttccacgtc
    21481 tttttccgtc agctgccgga gatgggggtt gcccttccag atatcgcaca tcacgcaccg
    21541 gcaattgcag gcgctgtggg gcataaggat aacgatcggc agggcggata ttctgtcggt
    21601 ctggagtgtc cggtaacgtc gtaacgtatg cagcaacggc ggaaaatgca tcgtcttcaa
    21661 ttatttctta tacgaatcca gggaaaaggg gccttcgacc ggaaaaaccg gttgcggaaa
    21721 tctttaggat tatagaggat ggtataatgg aaagaacgaa tatcggtgaa agaatgatgc
    21781 gaataatagt ccgccatctc ggggtagccg aaagacgtat aatacgccag ggtgcggcgg
    21841 gccgccagag gcacttcctc gggccggtaa aagggcgtat cgagcagatg gatctcaccg
    21901 cccggcttca gatacgacaa acacagatga agtatttttt ttgccgaagg gaaatactgc
    21961 agggaggcgg caaacaggat aacatcgaag gttcggtctt ccagtatccc cgaacggata
    22021 tccccgtgga tgaaacgcag attaggatcg ttattgaata cccgggccgc ctgctgcagc
    22081 tcggtaaaat tgatatcgac tcccgtgacc ctcgtacccg gcagctcggc taattggtgc
    22141 gacagccagc cattgccgca acctatctcg aggatgtcca tcgaggctct gcgggtggac
    22201 aggtaccgga gaagacggcg ggtggatcgc ctcctggttt gccattccct gaaatggggg
    22261 tgtccgggtt cgatgtcggg cagatggagc aattcctggt ccgtatacag tctgccttcc
    22321 agcgatcgca cctggatata ttgtttttcg aacaacatct cccccaacct ggtgtgaccc
    22381 tggacctgaa tattggttaa aatggaggaa tccggtaggt tcattttcaa gattttagct
    22441 aatgtttagg gaaccgctag aataaacgtc ggtttgatcc caaaggatgc ccaaagcact
    22501 aaaaaattcg gggttgacta cacccttttc ccattgaggg acaactgttt cgtctgccat
    22561 ttttaaaaac cctgtatctc cggctgacgg taacgccaga tcttatgcag cagttttatt
    22621 tcataagggt gtttgtacag atctgtccga tagcggatcg ccgacaacaa ccgtactacc
    22681 ttccttttca gggaattcag ccggatatcg gaaacggtcg gataataccc gttcaatacg
    22741 gtttcgaaat ctcttatttt gtcgatcatc tccggtcgaa gccagggtgt gagcgggttt
    22801 ttccgcagat cgaatcgctc ccactgcggc gtcatccagt cctccagctt ttccggatag
    22861 gtaaacccgg ctttttttac ctgttcgaac aattcggaac cttcggtagg aaccgggctg
    22921 taagtataca atacgatttc ggtggatgga ttgacggcct tcaccttctt gataaaattg
    22981 atatcgaaat ctatctgctc catgaccttt tcgggcgtcg gggcgggtac ccccagcaca
    23041 aaagagtatt cgggtatgat gtcatacttt ttcaaccgct gggcaaaacg caggatctgc
    23101 gcgccacttt gcgtgccgcc cttatccagc tggttcaaaa ccgagtcgtt gccgctctcc
    23161 gctccgaaga aaatgatcct gcatcccgct tcgcggatga gggccagcga ttcgtccttc
    23221 catttgtcca tcgtgtcgat cctcgccata ccccaccaat tcatcttctc aggaaggatc
    23281 agtctggcga attccgttac acgtttctcg gaaacgaaaa aattattatc gtgaaattcg
    23341 atgccgtcgg ctccccagtt gtcctttatg aatttgatgt cccgatagac ctgttcggcc
    23401 gtcttggcct tccatcttgc ttcatagatg ggaaccaccg cgcagaatga gcacgtgaat
    23461 ggacaaccga tgctggaatg gtacgccaac gttttgtcgc ccag
//
LOCUS       DASYSV010000464         8365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2142787, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000464 DASYSV010000000
VERSION     DASYSV010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142787"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3061
                     /locus_tag="VGQ51_14320"
     CDS             <1..3061
                     /locus_tag="VGQ51_14320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9037805.1"
                     /translation="IYVAYNSTSNVFGGVTTFNNTPTANTGIYVSWNSAGTSFSNNII
                     VGSNNGQGVQFCGGNATATATLSAGNTISIGPAGFSSGTLLLRHFTQVGATPQSLTLT
                     GTGNLTYGPSSSFDGNVNSSSPTLFFNGCTFNGTTICTKTGTTGDYSQGGNVFNGVSS
                     ITNSGTSFLLMGNVNADIWNNDVTFTDNGSERLLPCWATTGNQFNGNIYVNTAGSAQG
                     IEFCGGSTTATATLAAGKSIQAGGTGLNAGYLILKQFTQLGNSPISLALASTATYLQF
                     GPSSSLGGNVVSTSPGLYFNGCTFNGAVTSTKTGSTNDNSSGNNIFNGITTITDAGAG
                     YLLLGNGNSDQFNNTAAFNNTGTSTVYVAYNSPNNIFGGVTTFSNTPGANTGIYVSSY
                     SAGTTFTNNILVNSTAGTGVQFCTGNSSATATLNAGNTISVGTIGFTAGTLLLRQFTQ
                     LGATGQNITLTGTGTLTFGPSSTFNGPTTTSSPSLFFNVSTFNNNVNSTKTGTTNDQS
                     IGNNIFNGTNLFTNNGTGYLMMSTSATDAYNGDVAFVKAGSGAVYPNYNANCTYSGNL
                     TVTSPAASAITFGAGSGTATFTGSGGQNIYATAGTATPVFTRLVINNTGSGVTLFNTS
                     INVSSNLTLTSGLLNTSTAYILTMLNGSATAAGTALSTSYVNGPMRYQKSSAGSTALN
                     FPIGTAPDCRPVVLTVSHTNGTLYTYTVQLYNASAYALGYTMPPSVQIVSSMHYYIIG
                     RTDASGTNQPTAGLSGNQTIQIFFGANDYVTDGSTLTIVKNTYLTPTKWIDIGGTGAP
                     PYSGGADLIGSIISTSSPTAFNSFSTFALANRIGGSNVLPMKLLYFNAKPDNTQVDLT
                     WETATESNNDYFTVEKSKDGISFDDLTRVNSAAPNGTSSLPLYYSAVDPKPYTGTTWY
                     RLRQTDLNGNSTWSNIVSVDFSKTSAVSVYPNPSTGIVYIDGLGNNQSIASVQWFDLG
                     GRLLMQQTASITGGTTRLTTHFNNGVYILRFATADGTIITKTILIMK"
     gene            complement(3067..5913)
                     /locus_tag="VGQ51_14325"
     CDS             complement(3067..5913)
                     /locus_tag="VGQ51_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEV9037806.1"
                     /translation="MKKNKDDIGKALSAILPADRIKTRYIDLVTYGSDAGFYYLLPRA
                     VVQPVSEGEIVALFEFSHRLRIPLVFRAGGTSLSGQSITAGILVEIGRHWNRINITDS
                     GQTVEVGPGITGAMVNARLKAYGRKIGPDPSSIGSALMGGILSNNSSGMCCGVVNNSY
                     HTTRAVRFILPNGKTFDTGVSGERERFEKECPEISGELNQLSAVLAADTSLRNRIRDK
                     YRIKNTVGYSLNAFLDYSHPLDILAHLLIGGEGTLGFIAGAVLETIPQYPCRSTALVY
                     FRDLYAACEAIPVLIETGGEAVELMDRASLRAVEDIPGMPPLIRELPAAAAALLVEYQ
                     DENDAALQAKIVAFMGHVPGLSLLHAPEFTTDVKEQDLLWKVRKGLFPSVGAVRKSGT
                     TVILEDIAFPPGRLGDALTDLRALFDKHRYPDAIIFGHAKDGNIHFVITQSFHTDEDV
                     RKYDVFIQEMVTLVVDRYGGALKAEHGTGRNMAPFVETEWGTDAYMIMRRLKRCIDPE
                     NLLNPGVIISDDERAHIRDIKQLPSVEQEVDKCIECGYCEHVCPSRDLTLTPRRRIVA
                     RRVLRLLEASGDRENHALLLEQYQYDGLDTCAVDGLCSAACPVDINTGDLVKRLRRED
                     HSRWGNRMALWVAKHFRTTERVVGAGLRIGAGINRLFGAKTMTRLTKGMRRIVPGMPV
                     WPVELGAVSSMPRGSGGSNGSVVYFPACVSRIMSVKGQSVMDAMVSVSRKAGIGVIIP
                     DTVKGACCGQLFSSKGYGPAFGYTANRIVDYLWEVTRQGKIPVVTDLSSCAYTLMHIR
                     GVLDTEGLQRYDKLVIMDSVDYLHDMVLPLAAEVRREEEVVLHPVCSLQKMKTYDKLV
                     SVARHFSKAVTVPLQAGCCGMAGDRGFLFPELTDAATRGEATEVRQREYDGYYSSAVS
                     CELALTQAVGKDYVSVLFLADRAMKDGDSDRPMR"
     gene            complement(5913..7259)
                     /locus_tag="VGQ51_14330"
     CDS             complement(5913..7259)
                     /locus_tag="VGQ51_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037807.1"
                     /translation="MQFKLVLRGVLIFAGVPFLATAQFPVGRIVDSVVCKADASQSYA
                     VYIPVAGGKEALPAVYFFDSHGAGALPLLKYRRLADIYGCILIGSNNSKNGNDWNTTE
                     TIWRRLSEDTHQRLKINGARLYTAGFSGGAKVASFVAIQHPEIKGVIVNGAGLPDGVS
                     AGDFPFSITAIAGEGDMNMTDLVALDGQLDKTRTRHRILFFDGKHEWAPETTMRTAFI
                     GWQLDAMEAKLIPRNEAFIDAWVSKSRQQEEAFERDSRLIKAWQECRLSVRLLEGLTD
                     ARWFREKAASLESNALYRKQRQQEQTLLVSEENTKADYMQHFQQGDRVYWAKTIDGLR
                     AKAVAKTAEGAMYQRLLAYLSLAFYSISNQMINAGQNAAARHFTDLYKMDDPTNSEAW
                     YFSAILDAREGNARAAQSDLATAVRYGFNDKRRLRQQPEFKQMRIDLSGPESKMHP"
     gene            complement(7292..7873)
                     /locus_tag="VGQ51_14335"
     CDS             complement(7292..7873)
                     /locus_tag="VGQ51_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037808.1"
                     /translation="MNRKLILLLAGVFCLRAVRAQIEFEKIDTTMKVGKTGYRINCRN
                     KEVGENQMSIRPIGFESPANQGMNFPVRGRISKAMVDDLNGDGVADLILFIYTDSAAI
                     HGTVLAFISDGTKSIVPAGLPDVALDGKLNGGYKGHDQFSMLEGTLLQRFPIYKPGDK
                     DVPTGGNRVIQYQLSKVEGGGYKFAEMRSYDTH"
     gene            complement(7890..>8365)
                     /locus_tag="VGQ51_14340"
     CDS             complement(7890..>8365)
                     /locus_tag="VGQ51_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEV9037809.1"
                     /translation="GDIVLMDDGCKVQGYCSDISRCIVYGAEPTKRQREIWDLEKRAQ
                     AAGFAAAKVGAPCENVDAAARKVITDAGFGPGYKIPGLPHRTGHGIGMDIHEWGNMVK
                     GNKAPLLPGMCFSVEPMVAIYGEFGVRLEDCAYMTEDGPRWFSEPSPAIDRPFAS"
BASE COUNT         1887 a         2678 c         1993 g         1807 t
ORIGIN      
        1 catctatgtt gcgtataaca gcacgagcaa cgttttcggc ggggtcacta ccttcaataa
       61 tacacctacg gccaatacgg gcatatatgt cagctggaac tccgccggta cgtccttcag
      121 caataatatc atcgtcggca gcaacaacgg acaaggcgtt cagttctgcg gcggcaacgc
      181 caccgcaacc gccacgctgt cggcaggaaa taccatttcc atcggaccgg caggattttc
      241 atcggggacc ctgcttctcc gccattttac ccaggtcggc gcgacaccgc aaagcctgac
      301 actgacaggg acgggtaatc tgacctatgg cccttcttcc tccttcgatg ggaacgtcaa
      361 ctcctcgagc cctaccctgt tcttcaacgg gtgtacgttc aacggtacca ccatttgtac
      421 caagacgggc accaccgggg actatagcca gggcggcaac gtgttcaacg gggtcagctc
      481 cataaccaac agcggcacca gtttcctgct gatgggtaat gtcaacgcgg atatctggaa
      541 caacgacgtt acgttcaccg ataacggctc ggaacgcctc ctgccctgct gggccactac
      601 cggcaaccag ttcaacggga acatatatgt caacacggcc ggcagcgcac agggtatcga
      661 gttctgcggc ggcagtacga cggcgacggc cacccttgcc gcaggcaagt cgatccaggc
      721 tggcggcacc ggtctcaatg ccggatatct tatcctcaag cagttcaccc agctgggtaa
      781 ctcgcccatc agccttgcgc tggccagcac cgccacctat ctgcaattcg ggccctcttc
      841 ctcgctggga ggtaatgtgg tttccacgag cccgggtctg tatttcaacg gatgtacatt
      901 caacggcgct gtcacgagca caaaaaccgg ttccaccaat gacaatagca gtggtaataa
      961 tatcttcaac ggcataacca ctatcacgga cgcaggtgcc ggctacctcc ttttgggcaa
     1021 cggcaattcc gatcagttca ataatacggc tgctttcaac aacaccggca ccagcacggt
     1081 ctatgtggcc tataacagcc ctaacaatat tttcggcggg gtgaccacgt tcagcaatac
     1141 tccgggagcc aatacgggca tctatgtcag ctcctattcc gccggcacga cgtttaccaa
     1201 caatatcctg gtcaacagca ccgcgggaac cggagtccag ttctgtaccg ggaacagctc
     1261 ggccacggct accctcaatg ccggaaatac catttccgtg ggtaccatcg gattcaccgc
     1321 cgggaccctg cttctccggc agttcacgca attgggcgcc acgggtcaaa atatcacgct
     1381 gaccggcacg ggaactctta cattcggccc ttcctcgact ttcaacgggc ctactacgac
     1441 atcgagtccg tctttgttct tcaatgtctc taccttcaac aacaacgtca atagcacgaa
     1501 gacgggtacc accaatgacc aaagcatcgg aaataatatc ttcaatggca ccaacctctt
     1561 cacgaacaac ggtaccggct acctgatgat gagcaccagc gcgacggacg cttacaacgg
     1621 cgacgtcgcc ttcgtcaaag caggctccgg cgctgtctat ccgaactaca atgccaattg
     1681 tacctattcc ggtaatttaa ccgtcacctc gcctgcggct tcggccatta ccttcggcgc
     1741 cggcagcggt accgctacct ttacgggctc gggtggacaa aatatctatg cgaccgcagg
     1801 tacggctacc cccgtcttta cccggctggt gatcaataat accggtagcg gcgtgaccct
     1861 tttcaataca tcgatcaacg tgtcatccaa cctgacgctg acctcggggc tgctcaacac
     1921 ttccaccgcc tatatcctga cgatgctaaa cggctcggct accgcggccg gcacggcact
     1981 gtccaccagt tatgtcaacg ggccgatgcg ctaccagaaa tcatccgcgg gctcgaccgc
     2041 actcaatttc cccatcggta cggccccgga ttgcagaccg gttgtcctca ccgtcagcca
     2101 taccaacggg acgctgtata cctatacggt gcaactatac aatgccagcg cctatgcgtt
     2161 ggggtatacc atgcctccca gcgtgcagat cgtttcgagc atgcactatt atatcatcgg
     2221 caggacggac gcttccggaa ccaaccagcc aacggcgggt ctcagcggca accagaccat
     2281 ccagatattc ttcggtgcca atgactatgt gacggacggc agtacgctca ccatcgtcaa
     2341 gaatacctac ctgacgccca ccaaatggat cgatatcggg ggtacgggcg cgcctcccta
     2401 tagcggcggc gccgatctca taggcagcat catctcgacg tcgtcgccta cggccttcaa
     2461 ttcgttctcg acattcgctc tcgccaatcg tatcggcgga agcaatgtgc tgcccatgaa
     2521 gttgctgtac ttcaatgcca agccggataa tacgcaggtc gatctcacct gggagaccgc
     2581 taccgagtcg aacaacgatt atttcacggt tgaaaaaagt aaggacggca taagtttcga
     2641 cgacctgacc agggtaaatt cggcggcgcc caacggcacc agcagtctgc cattgtatta
     2701 ttccgcggtg gatccgaaac cgtacacggg caccacctgg taccgcctga gacaaaccga
     2761 tctgaacggg aattccacct ggtccaacat cgtatcggtc gatttctcca aaacgagtgc
     2821 ggtttcggtc tatcccaatc cgtcaacggg catcgtctat atcgacgggc tcggcaataa
     2881 tcaatcgata gccagcgtcc aatggttcga tctgggagga agactcctga tgcagcagac
     2941 agcctccatt acgggcggaa cgacacgact gacgacgcat ttcaacaatg gtgtctatat
     3001 cctgagattc gcgacggcag acggcaccat cattacaaaa acgatcctca tcatgaaata
     3061 acggcttcat cgcatcgggc gatcggagtc cccatccttc atcgcgcggt cggcaaggaa
     3121 caaaacggaa acataatctt tgccgaccgc ctgggtcagg gcaagctcgc agctgacggc
     3181 cgaagaatag taaccgtcat actcccgctg acgcacctcg gttgcctccc cacgcgtagc
     3241 cgcatccgtt agctccggga acaaaaaccc tctgtcgccg gccattccgc aacatccagc
     3301 ctgcagcggc acggtgaccg ccttcgagaa atgcctggcc accgacacca gcttatcgta
     3361 agtcttcatc ttctgcaacg aacaaacggg atgcaacact acctcttctt cccgccgcac
     3421 ctccgccgca agcggcagca ccatgtcatg taaatagtct acgctatcca tgatcaccag
     3481 tttgtcatag cgctgcaggc cctctgtatc caacacaccg cgaatatgca tcaacgtata
     3541 tgcgcatgag ctgaggtcgg tcaccacagg gatcttcccc tgccgggtga cctcccacaa
     3601 ataatcgacg atcctgttgg cggtataccc gaacgccggc ccatagccct tcgaagagaa
     3661 caattgaccg caacaggctc ccttcaccgt atcaggtatt attacaccga tacccgcttt
     3721 ccgcgagacg ctgaccatgg catccatgac cgactgccct ttcacgctca tgatccgcga
     3781 tacacacgcc ggaaaataca ctacactgcc atttgatcca ccggaacctc tcggcatcga
     3841 cgacaccgct cccaattcta ctggccaaac aggcattccc ggaacgatcc ttcgcatacc
     3901 cttcgtcagc ctggtcatcg tctttgcccc gaacaggcga ttgatacccg caccgatccg
     3961 cagtcccgca cccacaaccc gttcggttgt ccgaaaatgc ttagccaccc acaacgccat
     4021 cctgttcccc caccggctat gatcctcacg ccgcaatcgc tttaccaggt cgccggtgtt
     4081 gatatctacc gggcaggcgg ccgagcacag accatcgacc gcgcaggtat ccagcccgtc
     4141 atattggtat tgctccaaca gcaacgcatg gttctccctg tccccgctcg cctccagcaa
     4201 ccgaagcacg cgcctggcga cgatcctcct ccggggagtc agcgtaaggt cccggctcgg
     4261 acacacatgt tcgcaatatc cgcattcgat acatttgtcc acctcctgtt ccacggacgg
     4321 cagctgcttg atgtcccgga tatgcgcccg ctcgtcatcg gagatgatca ccccgggatt
     4381 gagcaggttc tccggatcga tacaccgctt tagccgacgc atgatcatat aggcatcggt
     4441 cccccattcc gtttctacaa aaggcgccat attcctcccc gtcccgtgct cggccttcaa
     4501 tgcgccccca taacggtcaa ccaccagcgt gaccatttcc tgaatgaaca cgtcgtattt
     4561 cctgacatct tcgtccgtat gaaaggactg cgtgatgaca aaatggatat tgccgtcttt
     4621 tgcatgcccg aaaattatgg catcgggata ccggtgctta tcgaacaacg cccgcagatc
     4681 ggtcaacgcg tcgccaagac gtccgggagg aaacgctata tcttccagta ttacagtagt
     4741 gccgctcttc cttacggccc ccacggaagg aaataatccc ttccgcactt tccacaacag
     4801 gtcctgctct ttcacgtcgg tggtgaattc cggagcatgc aacaacgaaa gacccggcac
     4861 atgccccatg aacgctacga tcttcgcctg caacgcggca tcattctcat cctggtattc
     4921 caccagcaaa gccgctgcag ccgccggtaa ttcccgtatc aacggcggca ttcccggtat
     4981 atcctccacg gcacgcaatg aggcccggtc catgagctcc accgcctccc cccccgtctc
     5041 tataagaacg gggatcgctt cacacgccgc atacagatcg cggaaataca ccaatgccgt
     5101 cgaccggcag ggatattgcg gaatggtctc caataccgct cccgcgataa atcccaacgt
     5161 gccttctccc ccgatcagca gatgcgccaa aatgtccagg gggtgcgaat agtcgagaaa
     5221 ggcattcagc gagtagccca ccgtattctt tatgcgatat ttatcgcgga tcctgttccg
     5281 caacgacgta tccgccgcaa gaacggccga cagctgattc agctctcccg atatctccgg
     5341 acattccttt tcgaatcttt cgcgctcgcc cgaaacaccc gtatcgaagg tcttcccgtt
     5401 gggaaggatg aaacgcaccg ccctggtcgt atgataggaa ttgttgacca ccccgcaaca
     5461 cataccgctc gagttgttgg aaaggatccc gcccatgagg gccgatccga tgctcgaagg
     5521 gtcagggccg atctttcgcc cgtacgcctt taaacgcgcg ttgaccatcg cacccgttat
     5581 ccccggtcct acttcgacgg tctgcccgct atcggtgatg tttatacgat tccaatgccg
     5641 gccgatctcg accaatatcc cagccgtgat cgattgcccg gaaagactcg ttcccccggc
     5701 acggaagacc aacgggatcc gcagtcgatg ggaaaattcg aaaagtgcga cgatctcccc
     5761 ttccgatacg ggttgcacta ccgccctcgg aagcagataa taaaaacccg cgtccgagcc
     5821 atacgtcacc aggtcgatat aacgcgtttt gatacggtcc gccggcagga tggccgataa
     5881 ggccttcccg atgtcgtcct tgttcttttt catcagggat gcattttgct ttcgggaccg
     5941 gaaagatcga tcctcatttg tttaaattcg ggctgttgcc gcaaacggcg cttgtcattg
     6001 aacccatatc ttacagccgt cgccaggtcg ctttgcgcag cgcgcgcgtt cccctcgcgc
     6061 gcatcgagga tggccgagaa ataccaggcc tcactgttcg tgggatcatc cattttatac
     6121 agatcggtaa aatggcgggc ggcggcgttc tgacccgcat tgatcatttg attgctgatg
     6181 gaataaaatg ccagtgagag ataggcgagc agccgctgat acatggcccc ttccgccgtt
     6241 ttcgcgacgg ccttcgcccg gagaccgtcg atcgtcttcg cccaatatac cctatcgccc
     6301 tgctgaaaat gctgcatgta atccgccttc gtattctcct cgctgaccag cagggtctgc
     6361 tcctgttgtc gttgcttccg atatagcgca ttcgattcca atgaagccgc tttttccctg
     6421 aaccatcggg catcggtcag gccttccaaa agacggaccg aaagccggca ttcctgccag
     6481 gcctttatga gtctgctgtc ccgctcaaat gcctcttcct gttgtctgct tttcgaaacc
     6541 caggcatcga taaacgcctc attgcgggga atgagcttcg cctccatggc gtccaactgc
     6601 catcctataa aggccgttcg catcgtagtc tccggagccc actcgtgctt cccatcgaag
     6661 aacaatatcc gatgccgggt ccgcgtcttg tctaattgcc catccaacgc taccaggtcg
     6721 gtcatattca tatcgccctc acccgcgatg gctgtaatgc tgaaagggaa atcgcccgcc
     6781 gaaacgccgt cgggcagccc cgctccattg acgatgacgc cttttatctc cggatgctgt
     6841 atggcaacga agctcgccac cttggccccg cccgagaaac ccgcggtata aagacgcgcc
     6901 ccgtttatct tcaggcgctg atgcgtgtct tccgatagcc ggcgccagat cgtttcagta
     6961 gtgttccaat cattgccgtt cttcgaatta ttgctgccga taaggatgca tccatagatg
     7021 tcggcaagcc tcctgtattt caacaacggt aatgcgcccg caccatgcga atcgaagaaa
     7081 tacacggcgg gtagagcctc tttcccgccg gccacaggaa tatagacggc gtaggattgc
     7141 gaggcatcgg ccttgcaaac gacgctatcg acgatccttc ccaccgggaa ctgggcagtc
     7201 gccagaaacg gcacgcccgc gaatatcagg acaccccgca ggaccagttt aaattgcatg
     7261 cttgttttta tttgcaagat gaagttgttc gctaatgcgt gtcgtacgac cgcatctcgg
     7321 cgaatttgta tccgccgcct tcaactttac tcaattgata ttggatcacc cggttgcccc
     7381 ccgtgggcac gtccttatcg ccgggtttat agatagggaa tctttgcaaa agcgtgcctt
     7441 ccagcatgga gaattgatca tgtcccttat agccgccgtt cagctttcca tccagcgcga
     7501 catcgggcaa tcccgccggt actatggatt ttgtaccatc cgagataaag gccagtaccg
     7561 tcccgtgtat cgccgcactg tcggtataga tgaacaggat caggtccgcc accccatctc
     7621 cgttcagatc gtccaccatg gccttcgata ttctccccct gacgggaaaa ttcatgccct
     7681 gattggcggg actctcgaaa ccgatgggcc ggatactcat ttggttctct ccaacctcct
     7741 tgttgcggca attgatccgg tacccggtct tgccgacttt catcgtcgta tcgatctttt
     7801 cgaattctat ctgggcccgg acagcacgca ggcaaaacac ccccgcgagc aaaagaatga
     7861 gctttctgtt catggcataa agatagggat cacgaagcaa atggcctgtc gatggccggg
     7921 ctgggttccg aaaaccaccg cgggccgtcc tccgtcatat aagcacaatc ttcgagcctc
     7981 actccgaact ccccatagat ggctaccatc ggctcgacgc tgaaacacat gccgggcagg
     8041 agcggagcct tattcccctt caccatattc ccccactcat ggatatccat cccgatgcca
     8101 tgacccgtcc tgtgcggaag accgggtatc ttatacccgg gtccaaaacc ggcatccgtg
     8161 atcaccttcc gggccgctgc atcgacattc tcacagggag caccgacctt cgccgcggca
     8221 aaccccgccg cctgggcccg cttttcgaga tcccagatct cccgctgacg ttttgtaggc
     8281 tccgccccat agacgatgca acgactgata tccgaacaat aaccctgtac tttgcaaccg
     8341 tcatccatga gcacgatatc gcctt
//
LOCUS       DASYSV010000465         6561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2018725, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000465 DASYSV010000000
VERSION     DASYSV010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018725"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VGQ51_14345"
     CDS             complement(<1..896)
                     /locus_tag="VGQ51_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEV9037810.1"
                     /translation="MGRKNIFAQKEQQAMQLARLQHYTRRHFLRESAMGLGGLALGAL
                     LGCHRTQNSVPVVFDPAHPLAPKLPPFPGKAKSVIYLHMAGAPSQLELFDYKPELMKM
                     DGQDCPPSLLEGKRFAFIRGVPKMLGPQAKFAQYGESRAWVSENLPHFSTMVDDVSFL
                     KAVSTDQFNHAPAQLLVHTGSPRLGRPSLGSWVTYGLGTENEDLPGFVVLTSGGKFPD
                     AGKSVWGSGFLPSVYQGVQCRSVGDPVLFLDDPAGMDRNLRKESIDAINAVNQQEFKD
                     YHDPEILSRIAQYEMAFRMQISV"
     gene            complement(900..3734)
                     /locus_tag="VGQ51_14350"
     CDS             complement(900..3734)
                     /locus_tag="VGQ51_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEV9037811.1"
                     /translation="MFSFLPKRLLIAVVLLAGAALVCWRVIRPARRVDFNTQVKPIIN
                     SKCITCHGGVRAKGGFSLLFREEAFAKTASGKPAIVAGDPDHSEMIRRITSKDPEIRM
                     PYKHEQLTKEEIDVLRDWVREGAPWGEHWAYKPVTAPNVPGLNDAWVRNDIDKYVLRK
                     LETEKLKPAAEAPRAELLRRVSLDLTGLYPSDGVAKAYLNGSDPQAYGMLVDSLLASP
                     HFGEKWAAMWLDLARYADTKGYERDDSRNIWRYRDWLIKAFNTDMPYDRFITEQLAGD
                     LMPHPTDADYIATAFHRNTMTNDEGGTDNEEFRTAAVLDRVNTTWEALLGTTFACTQC
                     HSHPYDPFHHEEYYQFMAYFNDTRDEDSYADYPLLRTFNDSDQTRLNSVVSWVSREGS
                     ADQAAAIKKLLRTWQPAINSLTCDQFVNSELEDTKWLAYRNNGSARMKAVDLKGDDNW
                     LIFRWRTEAPGGDWAIHMDSVRGRVIASARVEASGDWKIAAVPLKVEHGVHDLYVTYF
                     NPQLRKPDDKGLVFDWFCFTRQLPGSGRPGFKEAETAFWTLMTKNVPTTPIMMDNPAD
                     MHRVSNVFERGNWLVKGPVVKPGVPASLRACMPANAPANRLGLAMWMTSKQNPLVART
                     MVNRVWEQLFGTGIIESVEDLGTQGSPPTHRELLDYLAWKYVNDDQWSLKKLLKEIVM
                     SATYREDSRVTEESLKADPFDRLFTRGARVRLSAEELRDQDLCISGDLDPQMYGPSVF
                     PFQPKGIWNSPWNGAEWVASTNGEQYRRAMYTFWKRTASYPSMISFDATSRELCTPRR
                     IRTNTPLQALVTLNDTVYLDLARHFARRLDSLAADDPKREIEAGYRRMLYKDIEPDKL
                     AILLGLYRESQERFVRRPADARKMLGLMSDDDKGVAAPMRVTQVKQGGREGGQGGTGR
                     PAGGTQLADKAALVVVCNAMLNLDEVITRN"
     gene            3864..4853
                     /locus_tag="VGQ51_14355"
     CDS             3864..4853
                     /locus_tag="VGQ51_14355"
                     /inference="COORDINATES: protein motif:HMM:NF026866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 23 domain-containing
                     protein"
                     /protein_id="HEV9037812.1"
                     /translation="MHTLTRKLRVLWILSSFPLSGWGQTDLKTVRNDYRMEIGFSVRA
                     SLEFGGGQPKPNPILRLAGNAGVGAVFLDSWIYPTINQEFEFYNGGFGSSNRKDYYGG
                     FCLDAITSLTVTMGWPNRFVQTEALANRNIPLYYFADFIHPALQNPFDYSGSVGTNLI
                     FTTDAHKKSQRIGFINLHASRFQFCYYNDGGPPMSQTYLGDRSDRYYTGGGVIAVDLP
                     QNTALSSISLSYHKFTGYTRNAFELSNQLYLSYMNYHDESQKFYNKSLWSFSVANRQT
                     GLGVSLKSYNVVRLDMQAYIHSAIFNTFHIVPYPSWFAYDVNYTYSNNQIGVQ"
     gene            4850..5617
                     /locus_tag="VGQ51_14360"
     CDS             4850..5617
                     /locus_tag="VGQ51_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037813.1"
                     /translation="MRQVLLILLLFPAFGCKHNYYLFGRQGDVPPEDYRSIFLDSTYE
                     LYVRKVTKVGGEGHKDLESRQLIAATVESPLIEVEYLLLSDHHGNVIYFSTLADRFQN
                     LYQPYYLGDGYINTEDFETILFGKMLNSRTFLFFSGDTAKHSYVLWDWRKEAEGGKIN
                     SIQEFVKGEFQLFIPAAQALQDSIFFKHVGPFSIVHHYDTPDARDTIPLQDRTIHITR
                     DGKGWMVYFIFAQPIQNGYYNIKFLNDRVPFSPQIPQ"
     gene            5768..6232
                     /locus_tag="VGQ51_14365"
     CDS             5768..6232
                     /locus_tag="VGQ51_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037814.1"
                     /translation="MKMVGPNTSNYSYGVGAYVKGVFTVSQASDVTLELGANAFFLND
                     GGSGDGTAVVPIKVGYRYSLNGTGMGLYVEPQIGYNIYGVSSVNVDGYTKNLTFHGLV
                     LAAGTGYLFSIGNTPLDLHLRYETIVDHGGSDNYVSLGLSFYLHFGNRDNAQ"
     gene            6243..>6561
                     /locus_tag="VGQ51_14370"
     CDS             6243..>6561
                     /locus_tag="VGQ51_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037815.1"
                     /translation="MKQLLVPSLAILLLSSCLKQSIADAMIANEQSGTQGSGPGVATM
                     SYSVNGAAVTTSIKDPDSQSPTAYQLGCSKTLYPGTTSTIYSLDCLSNSGEMTFTFFT
                     DSLT"
BASE COUNT         1449 a         2001 c         1539 g         1572 t
ORIGIN      
        1 accgagatct gcatccggaa ggccatttcg tattgcgcga tccggctgag gatttccgga
       61 tcatgatagt ctttgaattc ctgctgattg acggcattga tcgcatcgat ggattctttc
      121 cgcagattgc ggtccatgcc cgcagggtcg tcgaggaaaa gcaccggatc gcccacgctg
      181 cgacattgca cgccctggta tacgctcggc aaaaaaccgc tgccccagac gctcttgccc
      241 gcatcgggaa atttgccgcc gctcgtcagc accacaaaac ccggcagatc ctcgttctcc
      301 gtccccagcc cgtaagtcac ccagctcccc agactcggcc tcccgagccg cggcgagccg
      361 gtatgaacca gcaactgcgc cggcgcgtga ttaaactggt cagtagacac ggccttcaga
      421 aaactcacgt cgtcaaccat cgtcgagaaa tgaggcaggt tctcgcttac ccacgccctg
      481 ctttctccat actgcgcaaa ctttgcctgc ggcccgagca ttttcggaac accgcggata
      541 aaggcaaacc gcttcccttc cagtaatgac ggcgggcaat cctgaccatc catcttcatc
      601 agctcgggtt tgtaatcgaa gagctcgagc tgtgacggcg cgcctgccat atgcagataa
      661 atgacgctct ttgcctttcc cggaaaaggt ggcaacttcg gcgccagcgg atgagccggg
      721 tcgaaaacca ccggtaccga attctgcgtc ctgtgacaac ccagcaacgc gcctagcgca
      781 agcccgccta atcccatcgc gctttccctg aggaaatgcc gccgggtata gtgctgcagc
      841 cttgccaact gcatagcctg ctgttccttc tgcgcgaata tattttttct acccataatc
      901 taattccttg tgatgacttc gtcgagattc aacatagcat tgcagacgac gaccagcgcc
      961 gctttatcag caagctgcgt ccctccggcc ggtcgccctg tcccaccttg cccaccttcg
     1021 cgcccgccct gcttcacctg cgtcactctc attggcgccg cgaccccctt gtcgtcatcc
     1081 gacatcaacc caagcatttt cctcgcatcc gccggtctgc gcacaaatct ctcctgcgat
     1141 tcgcggtaca accccagcag tatcgccagt ttatccggtt cgatatcctt atacaacatc
     1201 ctccgatatc ccgcctctat ctcccgttta ggatcatccg ccgcgaggct gtccagccgg
     1261 cgcgcgaaat gtctcgcaag gtcgagatag accgtatcat taagcgtgac cagcgcctgc
     1321 aatggcgtgt ttgtcctgat cctccgcggc gtacagagct cccgcgaagt ggcgtcgaat
     1381 gagatcatcg aaggatagga tgccgtccgt ttccaaaacg tatacatcgc ccgccggtat
     1441 tgctcgccgt tagtgctggc cacccattcc gctccattcc agggcgagtt ccagatgccc
     1501 ttgggctgga acgggaacac actcggccca tacatctgcg ggtcgagatc cccgctgata
     1561 caaaggtcct ggtccctcaa ttcttcggcg ctcagccgca cccgtgcccc gcgtgtaaaa
     1621 agccggtcga aaggatcggc cttcagcgac tcctccgtca cccgcgagtc ctcccgatag
     1681 gtagcgctca tcacgatttc tttcagaagt tttttcaagc tccactggtc gtcgttcaca
     1741 tatttccagg caagataatc gagcaattcc cggtgtgtcg gcgggctgcc ctgcgtcccc
     1801 aggtcctcca ccgactcgat aattcccgta ccgaacaact gttcccagac ccggttgacc
     1861 atcgtacggg ccaccagcgg attttgcttg ctcgtcatcc acatagccag cccgagacgg
     1921 tttgcaggcg cattcgccgg catgcacgca cgcaacgaag ccggaacacc cggcttcacg
     1981 accggtccct tgaccagcca gttcccccgt tcaaaaacat tcgaaacccg atgcatgtcc
     2041 gccggattat ccatcatgat cggcgtggtc ggcacattct tcgtcatcag cgtccagaat
     2101 gcggtttccg cttctttaaa accaggccgc cctgagcctg gcagctgccg cgtgaaacag
     2161 aaccagtcga acaccagtcc tttgtcgtca ggcttccgca gctgcgggtt gaaataggta
     2221 acgtagagat catgaacacc atgttcaacc ttcaacggca ccgcggctat cttccagtcg
     2281 ccactcgcct ccaccctggc cgacgcgatt acccgaccgc gtacactgtc catgtgaatg
     2341 gcccagtcgc cccccggagc ctcagtccgc cagcggaaga tcagccaatt gtcatcgcct
     2401 ttcagatcca cggccttcat ccgtgccgac ccgttgttcc ggtaggcaag ccattttgta
     2461 tcctcgagct cgctgttgac aaattgatca cacgtgagcg aattgatcgc cggctgccac
     2521 gtgcgaagta gtttttttat cgccgccgcc tggtccgcgc ttccttcccg cgaaacccag
     2581 ctcaccacgg aattcagccg ggtctggtcc gaatcgttga aagtgcgcag caagggataa
     2641 tctgcatagg aatcctcatc ccgcgtatcg ttgaaatagg ccatgaactg gtagtattct
     2701 tcatgatgga aaggatcata aggatggcta tgacattgcg tgcaggcgaa cgtggtgccc
     2761 agcagcgctt cccatgtggt attgaccctg tccagaaccg ccgccgtacg gaattcctca
     2821 ttatccgttc ccccttcgtc gttggtcatc gtattgcgat ggaatgcggt agcgatatag
     2881 tcagcatccg tcggatgcgg catcaggtcc cccgccaatt gctccgtgat aaatctgtcg
     2941 taaggcatgt ccgtattgaa agcctttatc agccagtccc tgtatcgcca gatattccgc
     3001 gagtcatccc gttcatatcc tttcgtatcc gcatatctcg ccagatccag ccacatcgcc
     3061 gcccacttct ccccaaaatg cggcgaagcc agcaggctgt ccaccagcat cccgtacgcc
     3121 tgcggatcgc ttccgttcag ataggctttc gccaccccgt cagacggata taacccggta
     3181 aggtccaggc tcacgcgtct cagcaactct gccctcggcg cctccgcagc cggcttcaat
     3241 ttctccgtct ccaatttccg cagcacatat ttgtcgatat cattccggac ccatgcatcg
     3301 ttcaaccccg gcacattcgg cgccgtcacc ggcttatagg cccaatgttc cccccacggc
     3361 gcgccttccc tgacccaatc ccgcagtaca tcgatctctt ccttcgtcag ctgctcatgc
     3421 ttatacggca tccgtatctc ggggtccttg cttgtgatcc gccggatcat ctcgctatga
     3481 tccggatcgc ccgccacgat cgccggtttc cccgacgccg tctttgcaaa agcttcctcc
     3541 cggaacagca ggctgaaccc acccttcgcc cgcaccccgc catgacaagt gatacatttt
     3601 gagttgatga ttggcttcac ctgggtgttg aaatccaccc tgcgtgccgg cctgataacc
     3661 cgccagcata ccagtgccgc ccccgccagc agcaccactg caattagcaa tcgtttcggg
     3721 aggaaagaga acatgtcgta atttatgaaa atccgcgcac ccgggcaaga attccgtgcc
     3781 cgccgcgggg tccggtcagc cgccgaaaat agggtctttt tacctttttt ggacctccgt
     3841 attagttgga catttatttt cacatgcata cgctaacgag aaagctgcga gtgttgtgga
     3901 tcctatcttc tttcccgctg tcgggatggg ggcaaacgga cctgaaaacc gtcaggaacg
     3961 actaccggat ggagatcgga ttttcggtca gggcttcgct cgaatttggc ggcggccagc
     4021 ccaaacctaa ccccatcctt cgattggcag gcaatgccgg agtgggcgcc gttttcctcg
     4081 acagctggat ctatccaact atcaaccagg aattcgagtt ttataacggc ggttttggca
     4141 gcagcaaccg gaaggattat tatggaggtt tttgtttgga tgccatcact tcgctgacgg
     4201 tcactatggg ttggccgaat cgttttgtcc aaaccgaagc ccttgccaac cggaatatcc
     4261 cgctttatta ttttgccgat tttatccatc ctgcgttaca aaaccccttc gattattccg
     4321 ggagtgttgg caccaatctg atctttacga ccgacgctca caagaagagt caacgaatag
     4381 gatttatcaa ccttcatgca agcaggttcc agttttgtta ctacaatgac ggcggtcccc
     4441 cgatgagcca aacctatctc ggcgatcggt cggaccggta ttataccggt ggtggggtca
     4501 tcgcggtcga tctgccccaa aacacggcgc tgtcctccat ctcgctctcc tatcacaagt
     4561 ttaccggcta tacgcgaaac gcattcgagc tttcgaacca gctttatctt tcgtatatga
     4621 actatcacga cgagtcccag aagttctaca acaagagtct ttggagcttt tcggtggcca
     4681 accggcagac cggtctcggg gtttcgctaa agtcttataa tgttgtcagg ctggatatgc
     4741 aggcctacat ccattccgcc atcttcaaca cctttcatat cgttccctat ccatcctggt
     4801 ttgcctatga cgtgaattac acctattcga ataatcaaat cggagtgcaa tgagacaggt
     4861 acttttaatc ttgctcctct tcccggcgtt tggctgcaaa cacaactatt atttgttcgg
     4921 ccggcaaggc gatgtcccgc ccgaggacta tcgcagcatt tttttggaca gcacttatga
     4981 gttgtatgtg cgtaaggtga caaaggtcgg cggagagggt cataaggacc tcgaatcccg
     5041 gcaactcatc gctgccaccg tcgagagccc ccttatcgaa gtcgaatacc tgctattgtc
     5101 ggatcaccac ggcaatgtga tctatttttc aaccctcgcc gacaggtttc aaaaccttta
     5161 tcaaccgtac tatctcggcg atgggtatat caataccgaa gacttcgaga ccatcctttt
     5221 cggcaaaatg ctgaatagcc gcacgttcct ctttttttcc ggcgacaccg ccaaacactc
     5281 ttatgtcctc tgggactggc gaaaagaagc cgaaggcgga aagatcaact cgattcagga
     5341 gttcgtcaaa ggcgaatttc agcttttcat ccccgccgcc caggccctgc aggattccat
     5401 ctttttcaaa catgtcggcc ccttttccat cgttcaccat tacgacactc ccgacgcccg
     5461 cgacaccata ccgctccagg atcgcacgat ccacatcacg cgcgacggca aagggtggat
     5521 ggtgtatttc atcttcgccc agccgattca aaacgggtat tataacatca agtttctaaa
     5581 tgaccgcgtc cccttctctc ctcaaatccc ccaatagcta agcagttaga gagtggtttg
     5641 catatctgca cgtaaactta tatcttggga cccgatctca atgatccaat ctaaagctat
     5701 gttgaaagga atattaacaa ccatcttatt cttgacagtt gcgagtgcag aagcccaact
     5761 ggggatcatg aaaatggtcg gacctaatac gagcaattat tcttatggag tcggcgcata
     5821 tgtcaaagga gtatttaccg tttctcaggc cagcgatgtc acactggagc tcggtgcaaa
     5881 tgcgttcttt ctgaatgatg gaggtagcgg tgatgggacg gccgtcgtgc cgataaaagt
     5941 aggctatcgt tattcgctga atggaacggg gatgggactc tatgtcgaac cccagatcgg
     6001 ttataatata tatggagtgt cgtctgtcaa cgtagatgga tacacgaaaa accttacgtt
     6061 tcatggattg gtactggcgg cgggcaccgg ctatttattt tcgatcggca ataccccgct
     6121 tgacctgcat ctgcgttatg agaccattgt cgatcacggc gggtccgaca attatgtaag
     6181 tctgggttta tctttttatt tacattttgg aaacagagac aatgcgcaat aaatattggg
     6241 cgatgaaaca attattggta ccctcactag cgatcctgct tctctcatcc tgtctaaagc
     6301 agtctatcgc agacgcaatg atcgccaacg aacaatccgg aacccaggga agcggccccg
     6361 gcgtcgcaac gatgtcctat tcggtgaatg gtgccgcggt aacaacctcc attaaagacc
     6421 ccgacagtca atcgcccacc gcttatcaat tgggatgtag caaaaccctc tatcccggca
     6481 caaccagcac tatctacagt cttgattgtc taagcaactc cggagaaatg acctttacct
     6541 tcttcaccga tagtctaacc a
//
LOCUS       DASYSV010000466         3872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2528515, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000466 DASYSV010000000
VERSION     DASYSV010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2528515"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VGQ51_14375"
     CDS             <1..731
                     /locus_tag="VGQ51_14375"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV9037816.1"
                     /translation="YSQHPDILCLEEFFDSQLPSDSCSIPYIQKRLQFPYYFFSRDYV
                     HKEGKYGGGVIIFSRWPIVDSLLMRYSRPDGFRATESLIAADIHAGKDTIRVFATHLQ
                     SVLFRGKDFHDLSIIRNVDDSILMASKSIAKKLGYAFRRRAEQAEQVRGRLDKSPYPG
                     IICGDFNDVPNSYTYAKIRDGWQDAFLKKGFGIGRTYVYISPTLRIDYILTNPAFSVL
                     QCRTFFSLQLSDHNPVVADVKLGN"
     gene            748..2253
                     /gene="cysS"
                     /locus_tag="VGQ51_14380"
     CDS             748..2253
                     /gene="cysS"
                     /locus_tag="VGQ51_14380"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV9037817.1"
                     /translation="MSDLKVYNSYSRQKEVFIPITPGHVGMYVCGPTVSGESHLGHAR
                     PFITFDFIYRYLLHLGYKVRYVRNITDAGHFEEEGREAEDKISKKAVVEKLEPMELVQ
                     KYTNLYHWAMHRFNNLDPSIEPTATGHIVEQIGMIEEIMRQGYAYEINGSVYFDVKKY
                     AAKYPYGKLSGRILDDLLETTRNLEGQEEKHNRQDFALWKAAPPEHIMRWASPWGEGF
                     PGWHIECSAMSTKYLGEEFDIHGGGMDLQFPHHESEIAQSTIANKHAPVKYWLHNNMI
                     TLNGKKMGKSYNNQIRLTEMFTGSHPLLQQAYSPMTIRFFILQTHYRSTLDFSNEALQ
                     AAEKGLKRLWEAYELLKKFAGEDAAGKPGTATDQALDEQVRRLLKELDEFMNDDFNTA
                     KVLANLFELVPVINSLKGGQIRRDAISPATLQLMQRYFTDYVENILGLKPETLGDDSK
                     LSGVMELLIDIRRDARSRKDYATSDKIRNQLQQLGIVLKDEKDGGMSYTFA"
     gene            2270..2965
                     /locus_tag="VGQ51_14385"
     CDS             2270..2965
                     /locus_tag="VGQ51_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEV9037818.1"
                     /translation="MEYLSHLSKDKKLKRLIEGQEPLNLDKSKDVPFYLILSIMSQQL
                     STKVADTLRKRFLALYEGRVPTPAEVLGTPFETLRSIGLSNAKTGYVHNVARFAVEQG
                     MDHRRLSKMDNEAIIAYLTQIKGVGRWTTEMLLMFCLGREDVFAIDDWGIQNAMIRLY
                     KLDRTDKKAFREKLMRISGRWSPYRTYACMHLWRWKDKSPATDGSAAGTATPAAVAAI
                     PAASAATPAVKKK"
     gene            3098..3643
                     /locus_tag="VGQ51_14390"
     CDS             3098..3643
                     /locus_tag="VGQ51_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037819.1"
                     /translation="MSKLKIVLLAAMAVSFVAMVSCSKSNNNGNGGSTSGSKDSVLYS
                     NWIPLHLVLTGAAADSDYEQKITASALTSSVLSKGLVLVYANEAGSGGQYVNYVSDFG
                     IYPTFGPQAIYLDAYGTYGYQISLNSVVDSVRYVIIPGNVSTTSVSGGLQTYTPSQLK
                     QLDYSSLSQILNIPAHGSSLH"
     gene            complement(3693..>3872)
                     /locus_tag="VGQ51_14395"
     CDS             complement(3693..>3872)
                     /locus_tag="VGQ51_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV9037820.1"
                     /translation="KRFVIAISKSDMLDDELKSAIEKELPARVSHVFISSVTQQGLTA
                     LKDLLWEVLNRENAS"
BASE COUNT          983 a         1034 c          991 g          864 t
ORIGIN      
        1 tctacagcca gcaccccgac attctctgtc tcgaggaatt cttcgactcc caattgccat
       61 cggacagctg cagcatcccc tatattcaga agcgtttgca atttccatac tacttctttt
      121 cgcgggatta tgtccacaaa gaggggaaat atggtggcgg cgttatcatc ttctcccgct
      181 ggcccatcgt agacagcctg ttgatgaggt attcccggcc cgacggcttc cgggcgacgg
      241 aaagcctcat cgcggcggat atccatgccg gaaaggatac catcagagtg tttgcgaccc
      301 atctccagtc cgtccttttc cgaggcaaag attttcatga cctgtcgatc atccggaacg
      361 tcgatgacag catcctgatg gcctccaaat ccatcgcgaa aaagctggga tatgccttcc
      421 ggcgccgggc cgaacaggcg gaacaggtac gcggccggct ggataaaagc ccgtatccgg
      481 gcatcatctg cggggatttc aatgatgtcc caaattccta cacttatgcc aagatccgtg
      541 acggttggca ggatgcattt ctgaaaaaag gtttcggcat cggcaggaca tatgtctata
      601 tctcccccac actgcggatc gactacattc tgacaaatcc ggcgttctcc gttcttcagt
      661 gcaggacatt tttttcgctt cagctgtcgg accacaaccc agtggtggcc gatgtgaaat
      721 tgggaaacta acgtaccttc gggacttatg agtgatctga aagtctacaa ttcttatagc
      781 cgtcaaaagg aagtattcat cccgatcacc ccgggccatg tgggaatgta tgtttgcggc
      841 cctacggtca gtggcgaaag tcatctgggc catgctcgtc ccttcatcac cttcgatttc
      901 atttatcgat atctgcttca tctcggatat aaggtccgct atgtccgcaa tatcaccgat
      961 gcgggacatt tcgaagaaga aggacgcgaa gccgaggaca agatctccaa aaaagcagtt
     1021 gtcgaaaagc tcgaacccat ggagctggta cagaaatata cgaacctgta ccattgggcc
     1081 atgcatcggt tcaataacct cgaccccagt atcgagccga ccgcgacagg ccatatcgtt
     1141 gaacagatcg gcatgatcga agagatcatg cgtcagggat atgcttatga gatcaacgga
     1201 tcggtatatt tcgatgtcaa aaaatatgcg gctaaatatc catacggaaa gctcagcggc
     1261 aggatcctgg acgatctcct tgaaaccacc cgcaacctcg agggccagga agagaaacat
     1321 aaccgccagg acttcgcctt gtggaaagcg gcgccccccg agcatatcat gcgctgggcc
     1381 agcccctggg gggagggatt cccgggctgg catatcgaat gctcggccat gagcaccaaa
     1441 tatctgggag aggaattcga cattcatggg ggcggtatgg acctgcagtt cccgcatcat
     1501 gagagcgaga tagcgcaaag taccatcgct aacaaacacg ccccggtgaa atactggctg
     1561 cacaacaata tgatcaccct gaatggcaag aagatgggaa agagctacaa taaccagatc
     1621 cgtctgacgg agatgtttac gggtagccac cctctgctgc agcaggccta ttcgcccatg
     1681 accatccgtt tcttcatcct ccagacccac tatcgcagca ccctcgactt cagtaatgaa
     1741 gctctgcagg ccgcggaaaa gggactcaaa cgcctctggg aggcatacga gctgctgaag
     1801 aagttcgccg gcgaagacgc ggccggcaaa ccgggtaccg ccaccgatca ggcactggac
     1861 gagcaggtgc ggcggctatt gaaagaactc gacgagttca tgaacgatga ttttaatacc
     1921 gccaaggtcc tcgccaatct cttcgagctg gtgccggtga tcaattcgct gaagggcggg
     1981 cagatccggc gcgatgccat cagtcccgcc accctgcagc tgatgcaacg ttattttacc
     2041 gactacgtgg agaatatcct ggggctgaag cctgaaacgt tgggcgatga cagcaagctc
     2101 agcggcgtca tggagctgct gatcgatatc cgccgcgatg cccgttcgag aaaggactat
     2161 gccaccagcg ataagatccg taaccagctg caacagctgg gcatcgtcct gaaggatgaa
     2221 aaggatggcg ggatgagcta tacgtttgcg taaaaccaat cactgtcgca tggagtacct
     2281 gtcccacctg tcaaaagaca agaaactgaa acgcctgatc gaaggccagg agcccctaaa
     2341 cctcgataaa agcaaagacg ttcctttcta cctcatttta tcgatcatga gccaacaact
     2401 atcgacgaag gtagccgaca cccttcggaa acgttttctg gccttgtatg agggccgggt
     2461 cccgactcct gcggaagtcc ttggcacacc ttttgaaacc ctccgttcca ttgggctttc
     2521 caatgccaaa accggctatg tccacaatgt ggcccgtttt gcagtcgaac agggcatgga
     2581 ccaccggcga ctatcgaaga tggacaacga agcgatcatc gcttatctga cacagataaa
     2641 aggcgtgggc cggtggacga cagaaatgtt gctgatgttt tgtctgggca gggaagacgt
     2701 cttcgccatc gacgattggg gtattcaaaa tgcaatgatc cgtttgtata aattggaccg
     2761 taccgacaag aaagctttcc gggaaaagct gatgcgcatc tccggcagat ggagccctta
     2821 ccggacctat gcctgtatgc acctgtggag atggaaagac aaaagtcctg ctaccgatgg
     2881 gtcagcagcc ggtacggcga cccctgcggc agtcgcagcg atccctgcgg catccgcggc
     2941 gaccccagcg gtgaagaaaa agtagtatct ttagataagc gggcattggc cggtaaaaat
     3001 tcgggtttga ccgattttct ttagactggc cgatccttaa atacgtattc cagaataaca
     3061 tacaatacaa tatctaataa cctttaaatg taatgttgtg agtaaattga aaatcgttct
     3121 cctggcggcc atggcagtat ctttcgtcgc aatggtcagt tgctcgaaaa gcaataataa
     3181 tggtaatggc ggctccactt cgggttcaaa agatagcgtg ctgtattcga attggatccc
     3241 gttgcatctg gtcctcaccg gggcagctgc cgattccgat tacgaacaaa aaatcaccgc
     3301 atcggcgctg acgagcagcg tcctgagcaa aggattggtt ttggtatatg caaacgaggc
     3361 tggatcgggc gggcaatacg tgaattacgt ttccgacttc ggcatctatc cgacgttcgg
     3421 accgcaggcc atctatctgg acgcttatgg cacctatggc taccagatct ccctgaactc
     3481 ggtagtcgac agcgtacgtt atgtcatcat acccggcaat gtctctacga ccagcgtttc
     3541 cggaggcctg cagacctata cgccatcgca gctcaaacag ctggactata gcagcctctc
     3601 gcagatcctg aacatccctg ctcacgggtc ctcgcttcac taaatgaatg aattgtaaag
     3661 aggccgtcga acgacggcct cttttgtttt agttaagacg cattctcccg gttcagcacc
     3721 tcccacagca aatcttttag cgcggtaaga ccctgttggg taaccgaaga tataaacacg
     3781 tgagagacac gggcgggcag ctctttttcg atagctgatt tgagctcatc gtcgagcata
     3841 tcgcttttgc tgatcgcgat gacaaatcgc tt
//
LOCUS       DASYSV010000467         4389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_993233, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000467 DASYSV010000000
VERSION     DASYSV010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993233"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VGQ51_14400"
     CDS             complement(<1..520)
                     /locus_tag="VGQ51_14400"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV9037821.1"
                     /translation="MRKVKLQVQITLDGFVGGPAGEMDYLVWNWDDRLKEYVTGLTDT
                     SDTILMGSILYQGMSGYWSAIPSDNEQYDFAQKMNNYSKLVFSTTLTGDLTWNNSRLA
                     KNDASAEIRALKESPGKDIMVYGGARFVSGLIKENLIDEYHLFVNPVAIGKGLSIFSG
                     LDGYFKLDLAAAH"
     gene            831..3500
                     /locus_tag="VGQ51_14405"
     CDS             831..3500
                     /locus_tag="VGQ51_14405"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV9037822.1"
                     /translation="MRNLVLAFVVSFLVVSTAFAQNPSQGNPGAKGAPAGARQAPPSI
                     GRAYGKVTDSTGQGIQATAVVLKSTVDPTTKKKKLVLLKGMDTKANGEFDFEELPIVA
                     QLVLRVSATGYKNHDVTFSIMPAQAAATPGQTNASPFGNLPSFDKDLGSLKLSLDVKQ
                     LATATVTGSAPPMKLELDKKVFNVDKNIVSAGGTGVDVMRNVPSVNVDIDGNVSLRGS
                     TPTIFVDGRPTTLTLDEIPADAIESVEVITNPSAKYDASGGGAGILNIILKKNRKKGY
                     NGSISAGMDSHGMPNALVGLSVRKDKVNFTLNGLYNGMNGKTVGNTDRTSLIPASATT
                     PGDSSHILQQDLNRNKGHFLFGQIGADWFVTNKTTLSASYIAVGGAFTPTDVSDIQTT
                     LLRTIGDSTYWSLRNSNSSRSFHVNGVQSGMKHIFNKKDEELTADFSYFGVNATLSSL
                     YTTNYFQGQPGTPIAATTQQKMVGTAAPGFYTIQTDYTDPLGSKTKLETGLRASIQKL
                     SNYNSTYTIDGTGKEHLDPYGVSDYTSTNTVYAAYVNFTSAINNFGYALGLRAESSAY
                     DGDLLNTVPPEHFSNKYPVSLFPSIFLSQKLKHNQELQISATRKINRPSFFNLIPFTD
                     YSDTLNITRGNPALVPEFTINTEMTYMKTFAHNNTLLISAYYKYTDNLITRFQDSGVS
                     PQGKAVLINTYKNANNAYTVGGEITSTDNITRWWDISINANVYNGHINTENLNEPSQP
                     AMWTFFGKFNSNFKLPASITIQMTAIYQGKTNLPVNLNNNQFGPPNTNTQSSSQGYIK
                     PYWGLDLAVKKTFLKNNAASVTVSMSDIFRTRWSDQYSTSQFFEQEYDRLKDPQLFRV
                     VFAYRFGKMDLNIFKRKDMNSQGMGDAGGSLQQ"
     gene            3805..4365
                     /locus_tag="VGQ51_14410"
     CDS             3805..4365
                     /locus_tag="VGQ51_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEV9037823.1"
                     /translation="MEIIRSIQNRIYEIRGERVMLDRDLADLYEVETRVLNQSVKRNL
                     KRFPSDFMFQLTQEEFESIRSQIEALEKSNSLKSQFVISNLPSRGGDRYLPYAFTEQG
                     VAMLSGILNSDKAINMNIAIMRAFVEVRHALLQPGDLRLKQLWERLNEHDVQLNQIYD
                     AMENLLDEKAAQRKWDDRERIGFKKR"
BASE COUNT         1133 a         1188 c         1051 g         1017 t
ORIGIN      
        1 aatgcgcggc ggccagatcg agtttgaaat agccgtccag acccgagaag atactcagcc
       61 ccttgccgat cgctacgggg ttcacgaaga gatggtattc gtcgatcagg ttttccttaa
      121 ttaagccgga aacaaaacgg gcgccgccat agaccattat gtccttaccg ggtgattcct
      181 tcaacgctcg gatctcggca gacgcatcgt ttttcgctaa ccggctgttg ttccaggtca
      241 ggtcgccggt taaagtagta gaaaacacca gcttactata attgttcatt ttctgcgcaa
      301 aatcgtattg ctcgttgtcc gaaggtatcg cactccagta acctgacatg ccctgataga
      361 ggatgctacc catcaggatc gtgtcggacg tgtccgtaag gccagtcaca tattctttca
      421 accgatcatc ccaattccaa acaaggtagt ccatctcgcc cgcaggaccg ccgacgaatc
      481 catccagtgt tatttgtacc tgcaatttta cctttctcat actttttgtt ttatattttc
      541 aaagctagtg gtaaaaatgc gcatcgatag ggtggaattg cgcaaaaaat agggggaatt
      601 gcgtagccgg tgtccaattt ctcatcattg gattgttatt attcgtcaga cggcgtaata
      661 tttatcacag atggcgtact aattatcgca gatagaggcg aaaatgtgtg ttcgccgatg
      721 acgtttgccg acagttttgt gccgtttacc gcgttttttg gtccgtttgc agtaatgcta
      781 taaagggtat cgcgacgcgc aattaatttt gttatcctaa aatatgtttt gtgagaaatt
      841 tagtacttgc tttcgttgtt tcttttctgg ttgtgtcgac cgcgtttgcc caaaacccga
      901 gtcaggggaa ccccggagcc aaaggcgctc cggctggcgc cagacaggct ccgccgtcga
      961 ttggacgtgc ttatggtaaa gtgacggact ccaccggcca gggcatccag gcgaccgccg
     1021 tcgtgctgaa gtccaccgtg gatccaacca ccaaaaagaa aaaactcgtg ttattgaagg
     1081 gcatggatac caaggcgaat ggtgaattcg atttcgaaga gctgccgatc gtcgctcaat
     1141 tggtgttgcg tgtatcggcc accggttata agaaccatga cgttaccttt tcgatcatgc
     1201 ctgcgcaggc cgcagccacg ccggggcaaa cgaatgcgtc cccgttcggc aatctgcctt
     1261 ccttcgataa agacctgggt tcgttgaaac tatcgctcga tgtcaaacaa ctggccactg
     1321 ccacggtcac cggcagcgct ccgccgatga agctcgaact cgacaagaaa gtattcaacg
     1381 tggacaagaa tatcgtcagc gccggcggta ccggcgtgga tgtcatgcgt aatgttccct
     1441 cggtaaatgt ggacatcgac ggcaacgttt cgcttcgcgg ctcgacaccg accatcttcg
     1501 tcgacggaag gcctaccacg ctgacattgg atgagatccc cgcggatgcg atcgaaagcg
     1561 tcgaggtcat caccaatcct tcggcaaaat acgatgcgtc gggtggggga gcgggcatcc
     1621 tcaacatcat cctcaagaag aatcgtaaga aaggatacaa cggaagcatc tccgcaggca
     1681 tggactcgca cggaatgcca aatgcactcg tcggtctcag cgtgcgcaag gataaggtta
     1741 actttaccct taacggtctc tacaacggaa tgaacggtaa gaccgtcggc aatacggatc
     1801 ggacatccct gatcccggcc agcgcgacga cccccggcga ttcttcgcat atccttcagc
     1861 aggaccttaa ccggaataaa ggacatttcc tttttggtca gatcggcgcg gactggttcg
     1921 tcacgaacaa gacgaccttg tcggccagct atatcgccgt cggcggcgcc tttacaccta
     1981 ccgatgtctc cgacatccaa accacgctgc tgcggacgat cggcgactct acctattggt
     2041 cgttgcgtaa ttcgaattcg agccgttctt tccatgtcaa tggcgtgcaa tccggcatga
     2101 agcatatctt caataagaaa gatgaggagc ttacggcaga cttctcctat ttcggtgtga
     2161 atgctacgct ctcttcgctg tatacgacca attatttcca gggccagccg ggtacgccga
     2221 tagcggcgac gactcagcaa aagatggtgg ggaccgccgc gcccggtttt tacacgatac
     2281 agaccgacta taccgatccc ctgggttcaa aaacaaaact cgaaaccggt cttcgcgcca
     2341 gtattcaaaa actgtccaat tacaatagca cctataccat cgatggtacc ggcaaggaac
     2401 acctcgaccc ttatggtgtc agtgattaca ccagcaccaa cacggtctat gcagcctatg
     2461 tcaattttac cagtgcgatc aacaatttcg gatatgcgct cggtcttcgc gcggaaagct
     2521 cagcctatga cggcgatctg ctgaataccg tccccccgga gcatttcagc aacaaatacc
     2581 ccgtcagcct gttcccctcc attttcctga gtcagaagct gaaacacaac caggagctgc
     2641 agatcagcgc aacgcgcaag atcaaccggc ccagcttctt caacctgata ccctttaccg
     2701 actattccga cacgttgaat attacgcgcg gtaacccggc gctggtacct gaattcacga
     2761 tcaatacgga aatgacgtat atgaagacct tcgcccacaa caataccctg ctgatatccg
     2821 cttattacaa gtatactgat aacctgatca cccggttcca ggacagcggc gtcagccccc
     2881 agggtaaggc ggtactcatc aatacctaca agaacgccaa caatgcatat acggtcggcg
     2941 gggagatcac gtctaccgac aacattacaa ggtggtggga catatcgatc aatgcgaacg
     3001 tttataacgg gcatatcaat acggaaaacc tgaatgagcc gtcgcaaccc gcaatgtgga
     3061 ccttcttcgg aaagttcaac agcaatttca agctgccggc cagcatcacg atccagatga
     3121 ccgctatcta tcagggtaag accaatcttc cggtgaacct gaacaacaat cagttcggcc
     3181 cgccgaacac caacacacag agctcgtccc agggttatat caagccctac tggggtctgg
     3241 acctggccgt gaagaagacc ttcctgaaaa acaatgccgc ctctgtcact gtcagcatga
     3301 gcgatatatt ccgcacgcgc tggtcggatc aatatagtac cagccagttc ttcgagcagg
     3361 agtacgatcg gctcaaagac ccgcaactgt tccgcgtggt attcgcctac cgcttcggaa
     3421 agatggattt gaatattttc aaaagaaagg acatgaattc gcaaggcatg ggtgatgcgg
     3481 gaggtagttt acagcaatga caatgacctc cacgcataaa aaggcccctc gcaagagggg
     3541 ctttttcatt ttatttgccg ggccaaccag gcgcggcaaa gtcgcgtatt gccggtgagg
     3601 gtggcccccg cggccgttcc cgaaaaaaat gattgccccg cgggcaacgc ttttcgcgga
     3661 atccgtatct cccctataat aatctcggcg tgcgttataa ttttacccgt cgcggcggcc
     3721 tgcgatgatg cagctgcccc aaacccattt ttttcttccc gatttccctg aatgatttaa
     3781 attcgaaggc tcaaatcggg ttctatggaa attatcagaa gcatccaaaa caggatatac
     3841 gagattcgcg gtgaaagggt catgctggac agagacctcg ccgatcttta tgaagttgaa
     3901 accagggtct taaatcagtc ggtaaaacgt aatttaaagc gctttcccag cgatttcatg
     3961 ttccagctga cccaggaaga atttgaatct ataaggtcgc aaattgaagc cttagagaag
     4021 tccaattctt tgaaatcaca atttgtgatc tcaaatctcc cctcccgcgg cggcgataga
     4081 tacctcccct atgcatttac cgagcagggc gtagctatgc tcagcggtat tctcaactcg
     4141 gacaaggcta taaacatgaa tatcgccatc atgcgggcgt tcgtcgaggt tcgccatgcc
     4201 ttactccaac cgggcgacct cagactcaag caactctggg aaagactgaa cgaacacgat
     4261 gtccaattaa accagatata cgacgccatg gaaaacctcc ttgatgaaaa ggctgcccaa
     4321 cgaaaatggg acgaccgcga gcggatcggt ttcaaaaaac gctgacaacc atcgccatcc
     4381 cttcctata
//
LOCUS       DASYSV010000468         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_95803, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000468 DASYSV010000000
VERSION     DASYSV010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95803"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VGQ51_14415"
     CDS             <1..1115
                     /locus_tag="VGQ51_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEV9037824.1"
                     /translation="GNFSTCTLGLGFIYDFTAYSQDQAFRQQMDTAGFSLGPTFKVRD
                     FRVLVSGVLKTKRPISWKFAYMYDGNLNTWLVRESGVTFGVPELKGDIFIGRTKEGFS
                     MVKVMNGHSPWTNERQMALDPIPILADGIKYFGYLPKSGIFWNLGAYTDWISKTQAFS
                     TFHWQYDARVGWMRYLNKENNELLHIAVNLRYGKPADGKFVIKSRPESNPTPQILNTG
                     NFATDHSSHLGYEIYYNRGSLLIGSEAMVHSFYSDTARNHRFFGGDVMIGWLFNHGRR
                     PYRTAGSLFGFVQVPRSVFAGGWGQWEAVVHLSTFNLNDQDIQGGQFWRITPMVNWYM
                     SKVMRFELIYGYGIFDRYDMKGKVQFFETRIQFTVM"
     gene            1149..2516
                     /gene="dcuC"
                     /locus_tag="VGQ51_14420"
     CDS             1149..2516
                     /gene="dcuC"
                     /locus_tag="VGQ51_14420"
                     /inference="COORDINATES: protein motif:HMM:NF015563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HEV9037825.1"
                     /translation="MNYVGLILSIAFITITAWLLVRKYNPQAVLLFMSIIMIALAGVF
                     HYPVPSLSEPSGLFVFDLFRRIQESLSDKTAGIGLMIMAIGGFVEYMKAIGASDALVL
                     VAMKPLGIFKRSPYIAASMVIPIGQVLFICTPSAAGLGLLLVASIFPVLVSLGVSRIS
                     AAAVISACTIFDMGPASANTARAAQLLEMHNVEYFIKHQLPLAIPMTVLLMVVYFLVN
                     RYYDRKIPPEQAKQPAVEESPLKVPVIYALLPVLPLILLIVFSPILHLFPDSVNLDTT
                     TAMLISLVLAMIMECIRLRSLQKVFASLQVFWEGMGGIFATVVTLIVCAEIFSTGLIS
                     LGFIDNLTYASMSMGLGGAGIGIVMTIIIYFASMLMGSGNAAFFSFGPLVPGIAAKLD
                     ESATDLILPMQFASSMGRGASPIAGVTVGIARIAGISPFDLARRNLIPLTVGVLFMLI
                     CHYSF"
     gene            complement(2557..>3322)
                     /locus_tag="VGQ51_14425"
     CDS             complement(2557..>3322)
                     /locus_tag="VGQ51_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEV9037826.1"
                     /translation="VHGIAFAHEVNKGFISDGRGQAVVVVDLKTFQIVKTIPLTATDE
                     DGIIYDPSSKKVFVFCGDSKSTCVIDPQTLTLVKTIDLGGGPEFAAADGKGLVYNNIE
                     DLSVMKVIDTKTMTVKDTWQLAPCGGPTGLAFDATHKRLFTGCRTNKGMSVVDATSGK
                     VITTVPIGAGVDAVVYDEPDHLIFCSNGDATTTIIRQESADKYSVVQTLATRTRAKTM
                     AMDRRTKKIYLSAPEFEPGTRKIVPGTFAVYVYKPA"
BASE COUNT          709 a          894 c          878 g          841 t
ORIGIN      
        1 acggcaattt ttcaacctgt acgctcggtc ttgggtttat ctacgacttt acggcctatt
       61 cgcaggatca ggctttccgg cagcaaatgg acacggcggg gttttcgctc ggcccgactt
      121 ttaaagtaag ggatttccgg gtattggtaa gtggagtgct caaaacaaaa agacccatct
      181 cctggaaatt cgcctatatg tatgacggca acctcaacac ctggttggtc agggaatcag
      241 gggtaacctt cggtgtccct gaattgaaag gcgacatttt tatcggtcgg acgaaagagg
      301 ggttttctat ggtgaaagtt atgaacgggc attcgccatg gacgaacgaa cggcaaatgg
      361 cgctcgaccc cattccgatc ctggcggacg gcatcaaata tttcgggtac ctgccgaaat
      421 ccgggatatt ctggaatctg ggagcctaca ccgactggat ctcaaagacg caagcttttt
      481 ccacttttca ttggcaatac gacgctcgtg tcggctggat gcgctatctg aataaggaaa
      541 acaatgaatt gctgcacatc gccgtcaatc tgcggtatgg caaacccgcc gatggaaaat
      601 tcgtcatcaa gtcccggccc gaatccaatc ctactccgca aatcctgaac acaggcaatt
      661 ttgcaacgga ccactcgagt catttgggtt atgagatcta ctataacagg ggcagcctgc
      721 tgatcgggtc cgaggccatg gtgcatagtt tctactcgga tacggcccgg aaccatcgat
      781 ttttcggcgg agatgtgatg atcggttggc tattcaatca tgggcgccga ccctatcgca
      841 cggccggcag cctgttcggg ttcgtgcagg tgcccagatc ggtattcgcg ggcggatggg
      901 gtcaatggga ggcggtagta cacctttcca ctttcaatct gaatgatcag gatatacagg
      961 gcggacaatt ctggcggatc acaccgatgg taaactggta tatgtcgaaa gtgatgcgtt
     1021 tcgaactcat ttatggctac ggcatatttg accgttacga catgaaggga aaggtgcaat
     1081 tcttcgaaac caggatccaa ttcaccgtca tgtagcgcaa gcgcgcaggc aatcttcaaa
     1141 ctcagaatat gaattacgtt ggcctgatcc tcagcatcgc atttatcacg atcaccgcct
     1201 ggctattggt caggaaatat aacccgcagg cggtgttatt gttcatgagc atcatcatga
     1261 tcgcgctggc cggagtgttt cattaccccg tgccatcgct gtcggagcct tccgggttgt
     1321 tcgttttcga tttgttccga cgcatccagg aatccctgtc ggataagact gcgggcatcg
     1381 gtttgatgat catggccatc ggcggtttcg tggaatacat gaaggccatc ggcgccagcg
     1441 acgccctggt acttgtagcc atgaagcctc tcggcatttt caaaagatca ccctatatcg
     1501 ccgcatccat ggtgataccc atcggtcagg tgctttttat ctgtacgcct tccgccgcag
     1561 gtctcgggct attgctcgtt gcgtctatct tcccggtgct ggtctcgctg ggcgtcagca
     1621 ggatttcggc ggcagccgtt atctcggctt gtacgatatt cgatatgggg ccggcctcgg
     1681 ctaataccgc ccgtgctgct cagctgctcg agatgcataa tgtggaatat tttatcaaac
     1741 accagttgcc gctggccata ccgatgactg tgctgttgat ggtggtctat ttcctcgtga
     1801 accggtatta cgaccggaag atcccgccgg aacaggcaaa gcaaccggcc gtcgaagaat
     1861 cgccgctgaa ggttccggtg atctatgcct tactgccggt gttaccgttg atattgctga
     1921 tcgtgttctc tcccatcctg catctttttc ccgatagcgt caacctggac accaccacgg
     1981 ccatgctgat ctcgctcgta ttggcgatga tcatggagtg catccgtctc cgcagtttgc
     2041 agaaggtgtt tgcctcgctg caggtattct gggaaggcat gggcggaata tttgccacgg
     2101 tggtgacatt gatcgtatgt gcggaaatat tttccaccgg gctgatatcg ctgggtttca
     2161 tcgacaacct gacctacgcc tccatgagca tggggctcgg cggagcaggc atcggcattg
     2221 tgatgacgat catcatttat ttcgcatcga tgctgatggg cagcgggaat gccgcatttt
     2281 tctcctttgg tcccctggtc cccggaattg ctgcgaaatt ggacgagtca gccacagatc
     2341 tgatcctccc aatgcagttc gcctcgagca tgggaagggg cgcatctcct attgcaggcg
     2401 tgaccgtcgg cattgccagg atcgccggga tatcaccctt cgatctggca cgcaggaatc
     2461 tgatcccatt gacggtgggc gttctgttca tgctgatctg tcactatagc ttttgagcta
     2521 atgaacccgg cgacggtacg agtcgccgca gaccgatcag gccggtttat aaacatacac
     2581 cgcaaaagtg cccggcacga tcttccttgt tccgggctcg aactccggcg cactcagata
     2641 gatcttcttc gtccgccgat ccatggccat cgtcttggcg cgtgtccgcg tagcgagggt
     2701 ctgtacgacg gaatatttat cggccgactc ctgcctgatg atggtagtag tcgcatcccc
     2761 attgctgcaa aagatcaggt gatctggctc atcatacact actgcgtcta ctccggcgcc
     2821 gatcggcacc gttgtaatca ccttcccgct ggtggcatcc acgacactca tccccttgtt
     2881 ggtgcggcat cccgtaaaca atcgcttgtg cgtcgcatcg aaagcaagac ccgtcggacc
     2941 tccgcaggga gccagctgcc aggtatcctt caccgtcatc gtcttcgtat cgatgacctt
     3001 cattacgctg aggtcttcga tattgttata taccaaaccc ttgccatcgg cggcggcgaa
     3061 ttcgggacca ccgcccagat cgatggtttt taccagtgta agcgtttggg gatcgatcac
     3121 gcaggtactc ttgctatctc cgcaaaagac gaacaccttt ttcgaggaag gatcgtatat
     3181 gatcccatct tcatcggtcg ccgtaagcgg gatggtcttc acgatctgga aggtcttcag
     3241 atcgacaacg accacggcct gtcctctccc atcgctgatg aaccctttat tgacctcatg
     3301 cgcgaatgcg atgccatgaa cg
//
LOCUS       DASYSV010000469         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2274429, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000469 DASYSV010000000
VERSION     DASYSV010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274429"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..682)
                     /locus_tag="VGQ51_14430"
     CDS             complement(182..682)
                     /locus_tag="VGQ51_14430"
                     /inference="COORDINATES: protein motif:HMM:NF027743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037827.1"
                     /translation="MRFKQFSLVTLSTLIVLTAGVTSCSKSGGGSSVNGVTATVGSTG
                     FSPAISVAFYTESGAMWEVGGYTIKSGDTTALVVSIPSPVTVNTKMSTTNSAIGVEYY
                     ISGSNKDYLAGPGWGSATLTVTSLDTTGHKIAGNFTGTLYNAMNSNDSIKVTNGQFNT
                     SYLLEP"
     gene            788..1825
                     /gene="dusB"
                     /locus_tag="VGQ51_14435"
     CDS             788..1825
                     /gene="dusB"
                     /locus_tag="VGQ51_14435"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV9037828.1"
                     /translation="MITIGNNILPDFPLLLAPMEDVSDPPFRAVCRSNGADLTFSEFI
                     SSESLIRGAIRSRRKLEIFGNERPVGIQLYGGDEDSLTMAAAIVETTGPDLLDINFGC
                     PVRGVVGRGAGAAVLRDPGRMVRLTEACVRATSLPVTVKTRLGWDNDSRNIVEVAERL
                     QDAGIHALTIHGRTRCQLYKGEADWELIGAVKNNPRMHIPIFGNGDIDSPQKALEYHN
                     RYGVDGIMIGRAAIGYPWIFREIRYYRRTGQLHPAPTVEERLAVCRQHLAGALAWNSP
                     LQGLLEMRGRYSRYLKGLPGIARYIQRLVRLPDAEAVEGVLQEIASAYEGYTTEAQPI
                     TLVNYHEHCAL"
BASE COUNT          452 a          543 c          595 g          438 t
ORIGIN      
        1 agggtcccgt cagtgggaag actattgttg tttttggtgc aggctacggc cagcaacagg
       61 gacgagagaa agagcacgat cggaaagggc ggattcttga ccttcattca acaaattttt
      121 tgtgaaggta caaaaaaggc cgtcctatgg acggccttta acaggatgat atgagaagcg
      181 attatggttc caggagataa gaagtattga actgaccatt ggtcaccttt atgctgtcgt
      241 tgctgttcat cgcattgtac agcgtaccgg taaaattgcc cgcgatctta tgacccgtcg
      301 tatccagcga ggtcacggtc agggtggccg aaccccatcc tgggccggca agataatcct
      361 tgttgctgcc gctgatataa tattctaccc cgatggccga attggtagta ctcatcttgg
      421 tattgacggt cacgggggag gggatcgaaa cgaccagcgc ggtggtgtct cccgacttta
      481 tcgtataacc gccgacttcc cacatagccc cggattcggt atagaacgcg acagagatag
      541 caggactaaa accggtggaa ccaaccgttg cggtaacacc gttgaccgaa ctgccgccgc
      601 cgcttttgct gcagctggtg acgcctgcag taagaactat caatgtggaa agggtgacga
      661 gtgaaaattg tttgaagcgc atactgtgat ttttttatac aggaatggaa aatactcccg
      721 aaaaacgtct tccgggtgca atttattgcc ggtgtccggg cccgaatttt agtctaactt
      781 gcagcggatg atcactatcg gaaataacat tctgcccgac tttccgctct tgctggcgcc
      841 catggaggac gtgagcgatc cgcctttccg ggctgtttgc cgctcgaatg gggccgatct
      901 caccttttcc gaattcattt ccagcgagag cctgatccgg ggtgctatcc gcagccgccg
      961 gaagctggag attttcggga acgagcgacc cgtaggcatt cagctctatg gcggggatga
     1021 agacagtctg accatggcag ccgccatcgt ggaaacgacc gggcccgatc tgctggacat
     1081 caatttcggg tgcccggtgc gcggcgtagt aggtcgcggc gccggagccg ccgtcttgcg
     1141 cgacccgggg cggatggtca ggttgacgga ggcctgtgta cgggcgacat cgctgcccgt
     1201 gacggtgaag acgaggctgg gatgggataa cgattcgcgg aatatcgtcg aggtggcgga
     1261 gcggctgcag gacgcgggta ttcacgcgct gaccattcac ggaagaacgc gttgccagct
     1321 atacaaaggt gaagcggact gggagctcat cggggccgtg aagaacaacc cccgtatgca
     1381 tatccccatc ttcggcaatg gcgacatcga cagtcctcaa aaagcgctgg aatatcacaa
     1441 ccggtatggt gtcgacggga tcatgatcgg cagggcggct atcggctatc cgtggatctt
     1501 tcgcgagatc aggtattacc ggcggacggg acagttgcat cccgcgccca cggtcgaaga
     1561 gcgtttggcc gtttgccggc agcacctggc aggtgccctg gcgtggaact caccgctgca
     1621 gggactactc gaaatgaggg gtcggtactc ccgttatttg aaagggctcc ccggtatcgc
     1681 ccgttacata caacgactgg tacgtctgcc ggacgcggag gcggtagagg gtgtgctgca
     1741 ggagatcgca tcggcctatg agggatatac cactgaggcc caaccgatca ccctcgtcaa
     1801 ctatcacgag cattgtgccc tgtaagggca taaaatccta tctttatcgg ccaaatgcta
     1861 aaccacgtgt acaaaacatc tttcctgctg accctcgtcc tcgctgcggc gacagcctgc
     1921 gcccagccgg ccaccgccac tatctatacc accgcggcca aaaccgatct gcggttgtcc
     1981 gaaaccgggc aattgcattt ttctcccgcg cgtcagccgt tcgaaacg
//
LOCUS       DASYSV010000470        14701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_993904, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000470 DASYSV010000000
VERSION     DASYSV010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 14701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14701
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993904"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..2560
                     /locus_tag="VGQ51_14440"
     CDS             74..2560
                     /locus_tag="VGQ51_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037829.1"
                     /translation="MIKYNLRLAWRQMFRHPLFTSIHVLGLSIGICACVVIFLIARHD
                     LGYDKFHPDGDRIYRIVGDMRFPDGSPMFLNSPFPQLAGLEHGIPGFEAQVGFHTWGQ
                     HIAVPGKPGEAPKEFSNSQEDSYASSTILTGPSFFNLFPHRWLAGSPSALDQPDKLVI
                     SESVAHRYFGDGPLEKMLGKTVIYDDSLPVTVAGIVDDWTRLSDLNYTSFISISTAPH
                     SWIRNQFPTADWSSLQPHRSQAFVRLARGVNPEEVNARMNDLVRKAHLVMFPGASAPH
                     LYLQSLYDIHYTPDFHPSDSGDDFRKAYLPLLYTLIGVAFFILLLALINFINLSTAQS
                     LQRVKEVGIRRVMGSSRKGLTIQLLVETFLLSLCAVVVSALLVRPALWLFGDYIPPGI
                     RFNPTDWGNISFLVVLLVFVTLAAGFYPARVLSSYRPVVSLKGAVASGREGLFGGSGL
                     RKGLIIFQFSISLVFIIGSLIIARQVRYMRDSDKGFDSDAILTVNQWRSKPGQMALLA
                     QTVSQLAGVKAVTVQSNAPMGFAHMGSNFIHEDKDGVKTLNVMVQGGDTAFIGVYGMK
                     LLAGRNMRPGDSVKEVLINETYARTLGFSSPDEATGKLIYQQHQQGKVAFTIVGVVAD
                     FHQESFHETIKPLVIQHEADLEHSVAVKLATKGKQADAARAIIAAMGPVWKSQFPKDP
                     FDYTFQSDLISRLYNQENNTAFLMGAAMILTIFVSCMGLFGLALYSAGRRAKEIGIRK
                     VMGASVRQIALLLSRDFLVLVVIALVIAAPVSWWLGHRWLEDFAYRAVIGVWVFVEAG
                     LAAVGLAVLTVGWQAVRAGRMNPVDTLRDE"
     gene            complement(2629..3981)
                     /gene="murF"
                     /locus_tag="VGQ51_14445"
     CDS             complement(2629..3981)
                     /gene="murF"
                     /locus_tag="VGQ51_14445"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV9037830.1"
                     /translation="MTTIEHLYHIYLQHPSVQTDTRLLLPGDLFFALKGPNFNGNHFA
                     TKALDSGAAYAVIDEVPASKDDRLLFVPNALTALQELALHHRRQFDIPFLAITGSNGK
                     TTTKELVHAALSAEYITYTTKGNLNNHIGIPLTLLSVRRDAEFAVIEMGANHQKEIEA
                     YCHYTLPTHGIITNIGRAHLEGFGGPEGVKKGKGELYDHLRDNGGTAFVWRDSDDLRQ
                     MSRGIPHIITYGAHDADFTGRVVPPAQPSTPGALLAASTFLHVEITHGASTGTIPTQL
                     VGDYNLPNILTAVAVGKHFGVPDGKIRRAIEQYTPSNSRSQLIEQGGNHIILDAYNAN
                     PSSMRAAIDNFARLPTAPTGNGSPSDGFGHKILILGAMAELGPESINEHRGIVDLIAR
                     YPWAKVVLVGGDFLKFDHPYTTFPDSREAAKWLQAAGLKDAWLLIKGSRSSKMELVLQ
                     "
     gene            4046..6517
                     /locus_tag="VGQ51_14450"
     CDS             4046..6517
                     /locus_tag="VGQ51_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037831.1"
                     /translation="MQFRHALGGQVGMFRNYLLTAFRHLRKDKTHSFINIAGLSIGMA
                     VALLIGLWITDELSFSKNFGHYDRIAQVFTNTTMNGERVTGRTVPVTLAEELRKHYGS
                     NFEQVVMTSWGNDHLLQSGTKVLTSQGSFMEEGGPEMLTLQMLQGSRDVLKQGSTIML
                     SRSLAAALFGNSDPMGRVVRFDNQLSLIVRGVYTDIPENSAFNELDYIVDWQSYLAAN
                     PWVPKMNDPWGNNSWRVFVQIAPNTDMATVSARIRDAKQKNVRDEEKKNHYALLLQPM
                     SRWNLYSEFKNGVNTGGRIEYVWMFGIIGVFVLLLACINFMNLSTARSERRAREVGIR
                     KAIGGLRSQLIYQFFSESMLLTLLAFGIALVLVSLSLQPFNAIAGKSVTMPWGSFSFW
                     AAGLGFCLVTGLIAGSYPALYLSSFRPVKVLKGTARFRTSLSVSKQLGRSSFKAGRFA
                     ALPRQVLVVVQFTVSVSLIIGTVIVFRQIQIAKDRPVGYDQNGLVMVPRITGTIHKHY
                     EAVREDLIHQGAVTDMAESSGPPTQVWSTNGGFDWPGKDPSQGVDNPNTGVSVNYGKT
                     VGWQFVAGRDFSKDFATDTSAFVINEAAVKFMGLKNPVGTVIKWDGYPFTIIGVIKDM
                     LMESPYSPVRPSFYCTIKDHEAVMILRVPAGANMAAALDKCKAVFTHYDPAEPFDYKF
                     VDKEYAKKFGDERRVGQLAGVFALLAILISCLGLFGMASFMAEQRTREIGVRKVLGAS
                     VVDLWGLLSKDFVLLVGLSLVIAMPLSAWVMKGWLAGYTIRTSLSWWIFAAAGAGALV
                     ITVVTVGWQAIRAAMANPVDSLRSE"
     gene            complement(6608..8158)
                     /locus_tag="VGQ51_14455"
     CDS             complement(6608..8158)
                     /locus_tag="VGQ51_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEV9037832.1"
                     /translation="MNLKNLAILASSVALFGSCNVFKKKPEQSTVTGWNYNDSKMGGF
                     QVSKEKEQRTGPGLVFIQGGTFTMGATQEDVMRDWNNVPRRVTVNSFYIDQTEVANIH
                     YREYLYWINNVFDADEYKAVREAALPDTLCWRSELAYNEPMVEYYFRHPSYNFYPVVG
                     VTWKQAHDFCLWRSDRVNEKLLIDRGYINANSVKNLQGLGQENFDTKAYLMGEFQATP
                     DKRARSKSNPLKNPNGTPRTQVTFEDGILLPNYRLPTEAEWEYAALGYVGQNPMPSHK
                     DKQRGEELIANKQVYSWSNNVNGLRDTRHGTWQGSFMANFKRGSGDNMGVAGGLNDNA
                     VYTAPVTSFFPNAFGLYNMSGNVSEWVADVYRPMTGQDADDVAPYRGNRFETVDYDKD
                     GKAQRDSLGRIKMRNVTDSESMNRRNYQKGDVINYLDGDSSISGINYGYGKTTLISNK
                     SRVVKGGSWNDRAYWLSPGTRRFLEEDQANSTIGFRCAMDRVGSPAGNGRQTGISYKQ
                     RRQNTRKQ"
     gene            8563..9738
                     /gene="porV"
                     /locus_tag="VGQ51_14460"
     CDS             8563..9738
                     /gene="porV"
                     /locus_tag="VGQ51_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HEV9037833.1"
                     /translation="MKRFYQGLAALLMLVFAGQGVFGQTNNLNVTTTAVPFLRISPDA
                     RAGGMGDLGVATSPDAASTFYNQSKIPFAKKDLGIGLTYTPWLKDLGLNDVYLLAASG
                     YKKLDALQALSFSIRYFSLGNIQFTDFSGNELGSGHPREFSFDAGYSRKLGDKLSVGL
                     AARYIYSNLASGYAASGSTYQPGKTFAADISLFYHGVTEDKGGWNFGLALTNLGGKIG
                     YTNSATDKDFIPADLGLGATYTTVFNEDNKLTFGIDMHKLLVPVPPTPTGDDSTDQSN
                     LDNYHNQSITGSWFKAFGGENQFKSLQFSLGAEYSYQDQFFFRAGYFYEDPSKGDLKY
                     FSLGIGVKYNVMGLNFSYLVPSGSGTNRNPLSNTLRFGLTFDLDSNGGDNNTSGSEQ"
     gene            9790..10263
                     /gene="ispF"
                     /locus_tag="VGQ51_14465"
     CDS             9790..10263
                     /gene="ispF"
                     /locus_tag="VGQ51_14465"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEV9037834.1"
                     /translation="MRIGFGIDYHQLAEGRELWLGGVRIPSAKGAVGHSDADVLIHAI
                     CDALLGALALGDIGQHFPNTDEAYKGIDSKILLSKTFEMVKASGWRVVNIDSTVTLEA
                     PKIMKYAVAMRETMAGILGVGVGDISIKATTTEKMGFVGRGEGLVAYATALLEKV"
     gene            10284..10724
                     /gene="dut"
                     /locus_tag="VGQ51_14470"
     CDS             10284..10724
                     /gene="dut"
                     /locus_tag="VGQ51_14470"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV9037835.1"
                     /translation="MSIKVKIINQSSNPLPEYATEGSAGVDLRANITAPVVLRPFERA
                     LVPTGLFIELPLGYEAQVRPRSGLALKHGITCLNSPGTIDSDYRGELKVILINLSQEE
                     QTLHHGDRIAQMVVSPVVQVGWVEVDSISETLRAAGGFGHTGKL"
     gene            10721..11584
                     /locus_tag="VGQ51_14475"
     CDS             10721..11584
                     /locus_tag="VGQ51_14475"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HEV9037836.1"
                     /translation="MNGIKILAGIVLAGVLAGAIGSCRSTKTIRKVIATPAPRPDTVG
                     AAKRAIEPVRDLHADSIAMIHSTINGLYRNHVDFQSFSGKIHVHFQSSDGKDYEVTAF
                     VRIKKDSLIWLSIVGSLGPVSKEVIRAMITPDSVKVRDNREKVIRMRSVSYLQEQVHL
                     PVDFKALQDLLIGNPIFLDTSRVLYYRTETKGISLFSAGAEFSNFLTLNPDLTMKHSK
                     VDDVDPLRARTCDLTYGDYDYSGVVPFSAYRKISVEEKSKVDIELGYKQYKFNEPLSY
                     PWQVPKNFKYR"
     gene            11623..12921
                     /locus_tag="VGQ51_14480"
     CDS             11623..12921
                     /locus_tag="VGQ51_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEV9037837.1"
                     /translation="MFKKIFIRCFGILFLTLAMGGRILAQAPGQTLPQSRADLEKERA
                     TIQKEMADLEKTLSETHRNKRENLGQLALLQRRLRLRESAIRNMNAQINAIQGDMNES
                     WREIIKLKAELDTLRVQYSQSIVFAYKNRSSYDFLNFIFSATSFNDALRRIEYLKSYR
                     AYREERAANIVRTQQLLQGKIDGLKVTRVEKEEAIKKQSKERSMLEDEKREKDAVVTR
                     LQSQEKELKKEMVAKQRQDAKLSSAISAVIRRATAAAAKENARKNAEAAASAARTGKE
                     EEVAPATLNTVKSGAKTVFSDDADVHLTGDFERNRGRLPWPVSGTISMAFGPHEYIKG
                     IIHNNLGVTIDVQAGAAVKAVFAGEIQAIFNVGDVQAVMVRHGRYFTTYSNLTTVSVT
                     KGEHITTGQILGRVGDVAQLEFAISNEKGTMLDPERWLKR"
     gene            13076..13564
                     /locus_tag="VGQ51_14485"
     CDS             13076..13564
                     /locus_tag="VGQ51_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037838.1"
                     /translation="MRENLFYRSRPGVSGVFVVLWRTICFASIILFVFLFDEIPGISV
                     MAIFGSFASLVAASRGVITVEEDRFIIGNKRIILELSDREEMPFSEIAEIEARLSVLR
                     QRLRISYEDGTVTSMTLFMDRAMVREALQTIRKHSTIRVEVVGNDIFRLADGRQGQEA
                     VV"
     gene            complement(13526..>14701)
                     /gene="bshA"
                     /locus_tag="VGQ51_14490"
     CDS             complement(13526..>14701)
                     /gene="bshA"
                     /locus_tag="VGQ51_14490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEV9037839.1"
                     /translation="DRKTYLSTYNSLPMRIGIVCYPTFGGSGVLATELGKALADKGHQ
                     VHFITYQQPVRLNEFNANIFYHEVRVTTYPLFDYPPYETALSSTMVDVINNHQLDLLH
                     VHYAIPHASAAYMAKKIVEAQGRYIPVITTLHGTDITLVGRDKTFAPVVAFSINQSDA
                     ITAVSQNLKDETFRNFSIEKDIKVIHNFVDTHRFHRKPIDAFRRVIAPNGERILLHAS
                     NFRKIKRVQDIVRIFEIVRKTIPSKLLFVGDGPERSDAESLCRELDICDDTRFVGKQE
                     QMEDILAVADLFLLTSEYESFGLAALEAMAAGVPVISTNAGGLPEIAIQGETGYLDDV
                     GDIRGMSEHAIGILSDDNILKKFKANAAAHAKNFDIHRIVPQYEALYNRFLTLSPVG"
BASE COUNT         3076 a         3404 c         4769 g         3452 t
ORIGIN      
        1 cgaaggcagg ttgtggaata cttcgttttg gcgtctggcg ggaatggcgg aaatttagat
       61 aaacgccttc cccatgataa aatataacct gcggctcgcg tggcggcaga tgttccgtca
      121 tccgctgttc acgtcgatac acgtgctggg tctttcgatc ggcatctgcg cctgtgtggt
      181 catcttcctc atcgcgcggc atgatctcgg ctatgacaaa ttccatcctg acggggaccg
      241 tatctatcgc atcgtgggtg atatgcgctt ccccgacggg tcgccgatgt tcctgaacag
      301 tccttttccg cagctggcgg ggctggagca tgggataccg gggttcgagg cgcaggttgg
      361 atttcacacc tggggtcagc atatcgcggt gccggggaag ccgggtgagg cgccgaagga
      421 attcagcaat tctcaggagg acagctatgc atcatcgacg atattgacgg ggccgtcgtt
      481 ctttaatctt tttcctcatc ggtggcttgc gggcagtcct tcggcgctgg atcagccgga
      541 caagctggtg atcagcgaaa gcgtggcgca tagatatttc ggtgacgggc cactggagaa
      601 gatgttgggg aagacggtga tctatgatga cagtctgccg gtgacggtgg cgggtatcgt
      661 ggatgactgg acgcggctat cggacctcaa ctataccagc ttcatttcca tatcgaccgc
      721 tccgcatagc tggatacgca atcaatttcc gactgccgac tggagcagtc tgcaaccgca
      781 ccggtcgcag gcttttgtgc ggcttgccag gggcgtgaac ccggaagagg tgaatgccag
      841 gatgaacgat cttgtccgaa aggctcatct tgtcatgttc cccggtgcaa gcgcgccgca
      901 tctctatctg caatcgctgt acgatatcca ttatacgccg gattttcatc cgtccgacag
      961 cggggatgat ttcagaaagg cttatttgcc gttgttgtat acgctgatag gtgtggcatt
     1021 ctttatcctg ctgctggcgt tgatcaattt catcaatctg tctacggcgc agtcgttaca
     1081 gcgggtgaaa gaagtgggca tacgcagggt gatgggcagc agccggaagg ggctgacgat
     1141 acagttgctt gtggagacct ttttgctgtc gctgtgcgcg gtggtagtgt ccgcgttgct
     1201 tgtgcggccg gcattgtggt tgttcggcga ctatatccca ccggggatcc ggttcaatcc
     1261 gacggattgg ggcaatatat cgttcctggt ggttctgctg gtgtttgtta cgctggcggc
     1321 cggattttat ccggcgaggg tcttgtcatc ctatcggccc gtggtgagcc tgaagggtgc
     1381 ggtagcctcg ggcagggaag gactatttgg cgggtcgggg ttgaggaagg gattgatcat
     1441 ttttcagttt agcatttcgc tggtgttcat tatcggatcg ttgatcatcg cgcggcaggt
     1501 gcgctatatg cgggactcgg acaagggttt cgacagcgat gcgatcctca ccgtgaatca
     1561 gtggcgcagc aagccggggc aaatggcatt gctggcgcag accgttagtc agctggcggg
     1621 cgtgaaagcg gtgaccgtgc agagcaatgc gccgatgggt tttgcgcata tggggagtaa
     1681 tttcatccat gaggacaagg acggcgtgaa gacgttgaat gtgatggtgc agggaggcga
     1741 tacggctttt atcggcgtat atgggatgaa actgctggcg ggccggaaca tgaggccggg
     1801 ggacagtgtg aaggaagtgc tgatcaatga gacctatgcc cggacgttag gtttttcatc
     1861 gccggatgag gcgacgggca agctgatcta ccagcaacat caacagggaa aagtggcttt
     1921 taccatcgtc ggagtggtgg cagactttca ccaggaatcg ttccatgaga ccatcaagcc
     1981 gctggtcatt cagcatgaag cagacctcga acatagcgta gccgtgaagc tggcgacaaa
     2041 gggtaaacag gccgatgcgg ccagggcgat cattgccgcg atgggtccgg tttggaagag
     2101 ccagttcccg aaggatccgt tcgattatac ctttcagagc gatctgatca gccggctgta
     2161 caatcaggag aacaatacgg ccttcctgat gggggcggcg atgatcctta cgatattcgt
     2221 gtcgtgtatg ggactcttcg ggttggcgtt gtattccgcc ggccggcgtg cgaaggagat
     2281 cggcatccgc aaggtgatgg gtgcgtcggt ccggcagatc gcattgctcc ttagccgcga
     2341 ttttttagtg ctggtcgtga tcgcgctggt gatcgccgcg ccggtgagct ggtggctggg
     2401 gcatcgttgg ttggaggatt ttgcgtaccg cgcagttatt ggtgtgtggg tattcgtcga
     2461 ggcggggttg gctgcggtgg ggttggcggt gctgactgtg gggtggcagg ctgtgagggc
     2521 cgggaggatg aatccggtgg atacgttgcg tgatgaataa gatgggttgg cggcggcggg
     2581 ttggcggtga cggcggcggg ttggcgcgcg tgacggcggc ggcgagggct attggaggac
     2641 gagctccatc ttgctgctgc gggagccttt gatgaggagc caggcgtctt tgaggccggc
     2701 ggcctgaagc catttggcgg cttcgcggct gtcggggaag gtggtatagg ggtggtcgaa
     2761 tttgaggaag tcgccgccga cgaggacgac cttagcccaa ggatatcggg cgatgagatc
     2821 gacgatgccg cgatgttcat tgatactttc ggggccgagc tcggccatgg cgccgaggat
     2881 gaggatcttg tgaccgaagc cgtcggaggg ggagccattg cccgttgggg cggtgggcag
     2941 gcgggcgaag ttgtcgatgg cggcgcgcat cgaggagggg ttggcgttat aggcgtcgag
     3001 gatgatatga ttaccgccct gttcgatgag ctggctacgg ctgttggagg gggtatattg
     3061 ttcgatggcg cggcggatct tcccgtcggg tacgccgaaa tgtttgccta cggctacggc
     3121 ggtgaggatg ttaggcagat tgtaatcgcc gaccagctgg gttgggatgg tgccggtgga
     3181 ggcgccgtgc gtgatctcga cgtggaggaa ggtgctggca gccagaaggg ctcccggggt
     3241 ggaaggttgc gctgggggca cgacacggcc ggtgaagtcg gcatcgtggg cgccataggt
     3301 gatgatatgg ggtatcccac gactcatttg ccggagatcg tcggagtcgc gccagacgaa
     3361 ggcggtgccg ccattgtcgc gaagatggtc gtagagctcg cccttccctt tctttacgcc
     3421 ttcggggccg ccgaaacctt cgagatgggc gcgcccgata ttggtgatga tcccatgggt
     3481 tggcagcgta taatggcaat aggcttcgat ctctttctgg tgattggcac ccatttcgat
     3541 gaccgcgaac tcggcgtcgc gccgcaccga caggagggtc agggggatac cgatatggtt
     3601 gttgagattg ccttttgtgg tataggtgat gtattcggcg gagagtgcgg catgcaccaa
     3661 ttctttggtg gtcgtctttc cgttgctgcc ggtgatggcc aggaatggga tatcgaattg
     3721 gcgacgatgg tggagcgcca gctcctgcag ggcagtgagc gcattgggga cgaagagcag
     3781 gcgatcgtct ttgcttgccg ggacttcatc gatgaccgca taggcggcgc cgctgtcgag
     3841 ggctttcgtc gcgaagtggt taccattgaa attggggcct ttaagggcga agaacagatc
     3901 gccgggtagg agcaggcgcg tgtcggtttg caccgagggg tgttggagat agatatggta
     3961 aagatgctcg atagtggtca tagcgtgtat cgaaagcggg caccccgccc gcgggcagcc
     4021 gggtaaaaaa atggtttcca aagatatgca attccggcat gcgcttggag gccaggttgg
     4081 catgtttaga aactatttac tcacggcctt ccgtcatttg cggaaagaca agacccattc
     4141 ttttatcaat attgccgggt tgtcgatcgg gatggcagtg gcgctgctga tcgggttgtg
     4201 gattacagat gagctgtctt ttagcaaaaa ttttggacat tatgaccgga tcgcccaggt
     4261 gttcaccaat accacgatga atggtgagcg ggtgacgggc cggacggtgc cggtgaccct
     4321 tgcggaggag ctgcgtaaac attatggcag caatttcgag caggtggtga tgaccagctg
     4381 gggcaatgac catctgctgc aatcggggac caaggtgctg acgagccagg gcagctttat
     4441 ggaagaagga gggccggaga tgctgacgct gcaaatgttg caggggagcc gggatgtttt
     4501 gaaacaaggg tctacgataa tgctttcccg gagcctggcg gccgcgttgt tcggaaacag
     4561 cgatccgatg gggcgggtcg tcagattcga taatcaactg agtcttatcg tgaggggcgt
     4621 ctataccgat atccccgaga acagcgcctt taatgagcta gattatattg tcgactggca
     4681 gagctatctc gccgccaatc cgtgggtgcc gaagatgaat gatccctggg gaaataacag
     4741 ttggcgtgtt tttgtccaga tagcgccgaa caccgatatg gccaccgtct ccgcgcgtat
     4801 ccgggatgcc aaacaaaaaa atgtgcggga cgaagagaag aagaaccatt atgcccttct
     4861 gcttcaaccg atgagccgct ggaatcttta ttcggaattc aagaatggtg tcaatacggg
     4921 cgggcgcata gaatatgtat ggatgttcgg tatcatcggc gtcttcgtgc tcctgctggc
     4981 atgtatcaat ttcatgaacc tgtctacggc ccggagtgag cggcgggctc gtgaagtagg
     5041 catccgcaag gcgatcgggg gattgcggag ccagctgatc tatcaattct tctcggagtc
     5101 gatgttgctg acactgttgg cgttcggtat tgcgctggtg ctggtcagtc tttcgttgca
     5161 gcctttcaat gcgatcgcgg gcaagtcggt cacgatgccg tggggcagtt tttcgttctg
     5221 ggctgcggga ctcggctttt gtcttgtgac gggattgata gcgggcagct atccggcatt
     5281 gtatctttcg tctttcagac cggtgaaggt gttgaaggga accgcgaggt tccgaacgag
     5341 cctgagcgtg tcgaagcagc tcgggcgaag ttcattcaag gcggggcgtt ttgcggcttt
     5401 gccgcgccag gtgctggtgg tggtacagtt caccgttagc gttagtctca tcatcggaac
     5461 ggtgatcgtg tttcgtcaga tacagattgc gaaggaccgg ccggtagggt atgaccaaaa
     5521 tggattggtg atggtgcccc ggatcaccgg caccattcat aaacattacg aagcggtgcg
     5581 ggaggatctg atacaccaag gggcggtgac ggatatggcg gagtccagcg gtcctccgac
     5641 gcaggtatgg tccaccaatg gcggtttcga ctggccgggt aaggacccga gtcagggtgt
     5701 agacaatccc aatacgggtg tgtcagtgaa ttatggaaag acggttggat ggcaattcgt
     5761 ggccggtcgc gatttctcga aggattttgc cacggatacg agcgcattcg tgatcaacga
     5821 ggcggcggtg aagttcatgg gattaaagaa cccggtgggg acggtgatca aatgggacgg
     5881 atatcctttc accatcatcg gggtgatcaa agacatgttg atggaatcgc cgtattcgcc
     5941 ggtgcgtcct tccttttatt gtacgataaa ggaccatgag gcggtgatga tccttcgtgt
     6001 gccggcaggc gcgaatatgg cggcggcgct ggacaagtgc aaggctgtgt ttacacatta
     6061 cgatccggcg gagccattcg attataaatt tgtggacaag gaatacgcga agaagttcgg
     6121 cgatgagagg cgggtagggc aactggctgg agtgtttgcg cttttggcga tcctgatcag
     6181 ctgtctcggg cttttcggta tggcctcatt tatggcggag cagcgtacaa gagagatcgg
     6241 tgtaaggaaa gtattggggg cgagtgtggt cgatctgtgg gggttgttgt cgaaggactt
     6301 cgtgctattg gtagggttgt cgctggtgat cgcgatgccg ttgagtgcgt gggtgatgaa
     6361 gggatggctt gcggggtata caatccggac ctcgttgtca tggtggatct ttgcggcggc
     6421 gggcgcgggg gcgttggtga tcacggtggt gacggtgggc tggcaggcga taagggccgc
     6481 gatggccaat ccggtcgata gtttgcgtag cgaatgaagg cgcttcgcgc cttccgggtt
     6541 tgcggaataa atgaacgaaa gaggcccggg caattgcccg ggcctcgtat ttttaaaata
     6601 ggaattatta ttgttttcgc gtgttctggc gtcgttgttt gtagctgata ccagtctggc
     6661 ggccgttgcc tgcggggctg ccgacgcggt ccatggcgca acggaatccg atggtgctgt
     6721 tggcctggtc ttcctcgagg aagcggcggg tgccggggct cagccagtaa gcgcggtcat
     6781 tccagctgcc gccttttact acacgggatt tattgctgat aagggtggtc tttccataac
     6841 cgtagttgat accggagatg gaggagtcac cgtcgagata gttgatgacg tcgcctttct
     6901 ggtagttgcg gcgattcatg ctttcagagt cggtcacgtt acgcatcttg atacggccga
     6961 gggagtcgcg ctgggctttg ccgtccttgt cgtaatccac cgtctcaaaa cggttgccac
     7021 gatagggggc cacgtcgtcg gcgtcctgac cggtcatggg gcggtagacg tcggctaccc
     7081 actcgctgac attacccgac atattataaa gaccaaaagc attggggaag aacgaggtta
     7141 cgggagcggt atagacggcg ttgtcattca gaccaccggc gacacccatg ttatcacccg
     7201 aaccgcgttt gaagttggcc atgaagctac cctgccaggt gccgtgacgc gtgtcgcgga
     7261 gaccgttgac gttgttgctc caggaataga cctgtttgtt ggcgataagc tcttcaccgc
     7321 gttgtttgtc tttatggctg ggcatcgggt tctgaccgac gtaaccaagg gcggcatatt
     7381 cccactcggc ctcggtcggg aggcgatagt tgggaaggag gataccgtct tcgaaggtga
     7441 cctgtgtacg cggggtacca ttggggttct tcagcggatt gctcttggat cttgcccgtt
     7501 tgtcgggagt tgcctggaac tcacccatca ggtaggcctt ggtatcgaag ttctcctggc
     7561 cgagaccctg gagattcttg acggagttgg cattgatata gccgcggtcg atgagcaatt
     7621 tctcattgac gcgatccgaa cgccacagac agaagtcgtg cgcctgtttc caggtcacgc
     7681 cgaccacggg ataaaaatta taggagggat ggcggaagta gtattcgacc atcggctcgt
     7741 tgtacgccag ttcgctacgc cagcagaggg tgtcgggtag tgccgcttcg cggactgcct
     7801 tgtactcatc ggcgtcgaag acgttattga tccagtagag gtattcacgg tagtggatat
     7861 tggctacttc cgtctggtcg atgtagaagg agttgaccgt tacgcgacgg ggaacgttgt
     7921 tccagtcacg cattacgtcc tcctgggtag cgcccatggt gaaagtaccg ccctggatga
     7981 ataccagacc cgggccggtg cgctgttctt tttctttcga tacctggaag ccacccattt
     8041 tggaatcgtt gtaattccag ccggtaacgg tcgattgctc cggttttttc ttaaaaacat
     8101 tacaggagcc aaagagggcg acggatgaag ccaggatagc taggtttttc agattcataa
     8161 gttgacgctt tttaaaccgt tgatgtccaa agacggcggg actgatggac ccgtcggctt
     8221 gaacgtggaa aacgacggtt ttgcctattt tattgtatga ccgccacatt atagtacggt
     8281 gtgaatttat ccacagttgc cgccgctgaa aattctggaa atttttcccg aaattctata
     8341 tttgccggcg aaaccgggcc aaacgcaatc cggtatggac tttgcaaaca ataggccaca
     8401 ataaacgatg attatatccg ttggagaaag attgaaaaag gggataattc cggcctcttc
     8461 ggaccggaac gacggatatg aatggattgc atatttaaat agttaaaaat cattaattta
     8521 gccgcctata tcacaaatct acttttaaat tttaacaagc tcatgaaaag attttaccag
     8581 ggattagctg ctttattaat gttagtcttt gcggggcagg gtgtttttgg acagacgaat
     8641 aatctgaatg tgacgacgac ggcggttccc ttcctgcgga tatcaccgga tgcccgggcc
     8701 gggggcatgg gtgatctcgg ggtagctact tcccccgacg cggcttctac tttctacaac
     8761 caatccaaga tacctttcgc gaagaaggat ctcggcatcg ggctgaccta tacgccctgg
     8821 ctgaaggacc tgggtctgaa tgatgtgtat ctgctggcgg ccagtggcta taagaagctg
     8881 gacgctcttc aggcgttgtc gttctcgatc cgatatttca gtctgggtaa tatccagttc
     8941 acggatttca gcggtaacga gcttggtagc ggccaccctc gcgaattcag ctttgacgcg
     9001 ggttatagcc ggaagctggg cgataagctg agtgtcggtc tggccgcccg ttatatctat
     9061 tccaatctgg cctcgggata tgccgccagc gggtcgacct atcagcccgg caagacattt
     9121 gccgccgaca tctcgttgtt ctatcacggc gtgacggaag acaagggggg ctggaatttc
     9181 ggtctggcgc tgacgaatct cggcggtaag atcgggtata ccaactcggc caccgacaag
     9241 gatttcatcc ctgccgatct gggtctgggc gcaacctata ccacggtatt caatgaagat
     9301 aataagctga cctttggtat cgacatgcac aagttgctgg tgccggtgcc tccgacgccg
     9361 acgggtgatg actcgaccga tcagtccaat ctggacaact accacaatca aagtattacg
     9421 ggcagctggt tcaaagcctt tggtggggag aatcagttca agagtctgca gttctcgctg
     9481 ggtgcggaat attcctacca ggatcagttc tttttccgtg cgggttattt ttatgaggac
     9541 cccagcaagg gcgatctgaa atatttcagc ctgggtatcg gcgtcaagta caatgtgatg
     9601 gggttgaatt tttcctattt ggtgccttcg ggcagcggga cgaaccgcaa tcccctgtcg
     9661 aatacgctgc gtttcgggtt gaccttcgac ctggacagca atggtggcga caacaatacg
     9721 tcggggagcg agcaataaat cgcgtttaag cttatatatt tgcggcggcc cttcgggccg
     9781 ccttttttta tgcgtattgg ttttgggata gattatcatc agttggccga aggccgggag
     9841 ttgtggctcg gcggggtgcg gataccttcg gcgaagggtg cggtcggaca tagcgacgcg
     9901 gacgtgctga tccatgccat ctgcgatgcg ttgctgggcg cgctcgcgct tggggatatc
     9961 gggcaacatt ttcccaatac ggatgaggca tataagggca tcgacagcaa gatattattg
    10021 agcaagactt ttgagatggt gaaggcctcg gggtggcgtg tcgtcaatat cgacagtact
    10081 gtgacgctgg aggcgccgaa gatcatgaaa tatgcggttg ccatgcgcga gacgatggcg
    10141 gggatcctcg gggtgggggt aggggatatt tcgatcaaag ccacgacgac ggagaagatg
    10201 gggtttgtag gacggggcga ggggttggtg gcgtatgcga ccgcgctttt ggaaaaggta
    10261 tgatgtagag gtatatttgc gccatgtcaa taaaggtcaa gatcatcaat caatcttcga
    10321 atcctctgcc tgaatatgcg acggaagggt cggcgggtgt ggatctccgg gcgaatatca
    10381 ccgcgccggt ggtgctcagg cccttcgaaa gggcgctggt ccctacgggg ctttttatcg
    10441 agctgccgtt gggttatgag gcgcaggtcc ggccccgcag cggtctggcc cttaaacacg
    10501 gcattacctg tctcaattcc cccggcacta tcgactccga ctatcgcggc gagctgaagg
    10561 tcattctcat caatttgtcg caggaagagc agaccttgca tcatggcgac cgtatcgcgc
    10621 agatggtggt gagcccggtg gtgcaggtcg gatgggtgga agtggattca attagtgaga
    10681 ccctccgcgc cgcgggtggg tttggacata ccggtaaatt atgaatggaa ttaaaatttt
    10741 ggcggggatc gttcttgccg gtgtgctcgc cggggcgatc ggaagctgcc gtagtacgaa
    10801 gacgatacgg aaagtgatcg ccactccggc cccgcgtcct gataccgtcg gggcggctaa
    10861 aagggcgatc gaacccgtcc gggatctgca tgcagactcg atcgccatga tccatagcac
    10921 gataaacggg ctttacagga atcatgtgga tttccagagt ttttccggta agatccatgt
    10981 gcacttccag agtagcgacg ggaaggacta tgaggtgacc gcgttcgttc gcatcaagaa
    11041 ggacagcctg atctggctct cgatcgtcgg gagcctggga cctgtgtcga aggaggtgat
    11101 ccgcgcgatg atcactccgg acagcgtcaa ggtcagggac aaccgggaga aggtcatccg
    11161 gatgcgttct gtaagctatc tccaggagca ggtacacctg cccgttgatt tcaaggcgtt
    11221 gcaggacctg ctgatcggca accccatctt cctcgacacg tcccgggtgt tgtactaccg
    11281 cacggagacg aagggcatct cgttgttcag tgcaggggcg gaattcagta acttccttac
    11341 gctgaacccc gatctgacga tgaagcatag caaggtggat gatgtggacc cgttgcgcgc
    11401 gcgtacctgc gatctgactt atggggatta cgattattcg ggggtggtac ccttctcggc
    11461 ctaccgcaag atctcggtgg aggaaaaatc gaaggtggat atcgagctgg ggtataagca
    11521 gtataagttt aacgaacctt taagctatcc ctggcaggtc ccgaagaatt ttaagtaccg
    11581 atagacgtta tacctggaca atcaccgcta aaaacagggt ccatgtttaa gaaaatcttt
    11641 atcaggtgtt tcggcatcct ttttttgacg ctggctatgg gcggacgcat cctggcgcag
    11701 gcgccgggac agacgttgcc gcaaagccgt gcggatctgg agaaggaacg tgcgacgatc
    11761 caaaaagaga tggcggatct ggaaaagacc ctcagcgaga cgcacaggaa caagcgggag
    11821 aacctgggtc agctggcgct gctgcagcgg cggcttcggt tgcgtgagtc ggccatccgg
    11881 aatatgaatg cccagatcaa cgcgatacaa ggggacatga atgagtcctg gcgcgagatc
    11941 atcaagctga aggccgagct ggatacgctc cgtgtccagt attcgcagag tatcgtgttc
    12001 gcgtataaga accggagcag ctatgacttc ctgaacttta ttttttcggc gacttcgttc
    12061 aacgacgctt tgcggcggat cgagtacctg aaatcctatc gggcttaccg ggaggagcgg
    12121 gccgcgaata tcgtgcggac gcagcagttg ttgcagggta agatcgacgg gctgaaggtg
    12181 acgcgtgtag agaaggagga ggcgatcaag aaacagagta aggagcggtc gatgctggag
    12241 gatgagaagc gggagaagga tgcggtcgtc acccgtctgc aatcgcagga gaaggaattg
    12301 aaaaaagaga tggtcgccaa acagcggcag gacgcgaaac tgagctccgc aatttccgcg
    12361 gtcatccgtc gcgccacggc cgcagcagcc aaagaaaacg cccggaagaa tgcagaggcc
    12421 gcggcatccg ctgccaggac agggaaggaa gaggaagtgg ctcccgccac gctgaatacg
    12481 gtaaagtcag gtgcgaaaac ggtctttagc gatgatgcgg acgtacatct gaccggggat
    12541 ttcgagcgga acaggggccg gttgccctgg ccggtgtcgg ggaccatttc gatggcgttc
    12601 gggccgcatg aatatatcaa aggcattatt cacaacaacc ttggcgttac gatcgacgtg
    12661 caggccggcg ctgccgtgaa ggccgtgttc gcgggcgaga tacaggccat tttcaatgtc
    12721 ggcgatgtgc aggccgtgat ggtgcggcat gggagatact tcaccactta tagcaatctg
    12781 acgacggtgt cggtgacgaa gggtgagcat attaccacgg gccagatact cggcagggtg
    12841 ggagatgtgg cgcagctgga gttcgctatc tccaatgaaa aggggacgat gctggatccg
    12901 gagcggtggt tgaaacggta gaggaagtac gcaagatatg gggcggccgg atgggacaca
    12961 atttataccc cttagggatc gtttaagagg taacacttag agggtcaccc gcggggcggg
    13021 agagacagtg cgtgagaagg cgggacccga agctaaaaat tcaattccta tgcgtatgcg
    13081 cgaaaacctc ttttaccgga gccgacccgg cgtgtccggt gtgttcgtcg tcctttggcg
    13141 gaccatctgt tttgcttcta tcatcctttt tgttttcttg ttcgacgaaa tccccggcat
    13201 ttcggtgatg gcgatcttcg gatcttttgc cagtctcgtc gccgccagca ggggcgttat
    13261 cacggtagaa gaagaccgtt ttatcatcgg gaacaagcgg atcatcctcg agttgtcgga
    13321 ccgcgaggag atgcctttct ccgagatcgc ggagatcgaa gcgcggctgt cggtattgcg
    13381 tcagcggctg cggatcagtt atgaggatgg gacggtgacg tctatgacct tgtttatgga
    13441 ccgggcgatg gtacgcgagg cgttgcagac catacggaaa cactcgacca ttcgcgtgga
    13501 agtggtggga aacgacatat tcaggttagc cgacgggcga caaggtcagg aagcggttgt
    13561 atagcgcctc gtattgcggc acgatgcggt ggatgtcgaa atttttcgca tgtgcggcag
    13621 cgttagcctt gaattttttc aggatattat cgtcgctgag gatcccgatg gcgtgctcgc
    13681 tcatgccgcg gatgtcgccg acatcgtcga gataacccgt ctccccctgt atcgcgatct
    13741 cgggcagccc ccctgcattg gtagagatca ccggtacgcc cgccgccata gcctcgagcg
    13801 cggcgagtcc aaagctttca tattccgaag tcagtaagaa cagatctgca actgcgagga
    13861 tgtcctccat ctgctcctgt ttgcctacga agcgggtgtc gtcgcagatg tcgagctcgc
    13921 ggcataggct ttcggcatcc gaccgttcgg gaccgtcgcc gacgaaaagc agcttgctgg
    13981 ggatcgtttt gcggacgatc tcgaagatgc ggacgatgtc ctgaacgcgt ttgatcttgc
    14041 ggaagttgga agcatgcagc aggatgcgtt ctccgttggg cgcgatgacc cggcgaaagg
    14101 cgtcgatggg tttgcggtgg aagcgatggg tgtcgacgaa attatggatg accttaatat
    14161 ctttttcgat gctgaagttg cggaaggttt cgtcctttag gttttgcgag acggcggtaa
    14221 tggcatcgct ttgattgatg gagaaggcca ccacgggggc gaaggtcttg tcacggccca
    14281 cgagggtgat atcggtgcca tgcagggtgg tgatcaccgg tatatagcga ccctgggcct
    14341 cgacgatctt tttagccata taggcggcgg aagcatgggg gatggcatag tggacatgca
    14401 gcagatcgag ttggtggtta ttgatgacgt ccaccatggt actggagagc gcggtctcat
    14461 agggaggata gtcgaaaaga ggataggtgg tgacgcgcac ctcatgatag aagatgttcg
    14521 cgttgaactc gttgagccga acgggttgct ggtaagtaat aaaatggacc tggtggcctt
    14581 tgtccgccag ggccttaccc aactcggtag caaggacgcc gctgccgccg aaagttggat
    14641 aacaaacgat gcctatgcgc ataggtagag aattgtatgt gctaaggtag gttttcctat
    14701 c
//
LOCUS       DASYSV010000471         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_868215, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000471 DASYSV010000000
VERSION     DASYSV010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868215"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="VGQ51_14495"
     CDS             complement(<1..1484)
                     /locus_tag="VGQ51_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037840.1"
                     /translation="MMSYSQAQSVGYSYDIRDSSLIPTKRLAQHNEFLNNSYPFPAKP
                     RNEWEIGVKGGYTNGFTSVHNWGPTGGFGIHIRKALGYMFSVRAEYDWIRLRGLNYQY
                     SQSYGRNPSLTQANLFPEQNSGSYYPIFYNYRSTVHELSFQGIFTINNINFHKAKIGW
                     NAYFFGGPGLMTYGTLYKQDDSYLAGYQNVINKYPLLDYKHRKDIISDLKNVLTGKWN
                     HIAERPSTSALLGGVPLTVVGVVGAGIQVKLTDKVNIALEDKFTFTATNLLDGQQWQN
                     NWTPGATNSVAQTSAKDRYNFVSLGINVNIFRKHAVEPLWWINPLDYAYNEINAPRHM
                     KLPKPVLDDTDGDGVTDQFDLEPNTPKGCPVDTHGVSRDTDGDGVPDCKDKELITPTQ
                     CQPVDADGVGKCPDPQCCKDLRDSLNAGVYGNGKGKCSIGDLPSITFKGRSVTLTKEQ
                     KALLASAAQKMRDNPTCKVAVIGYGESSKSAQQLSWDRVNAVIN"
     gene            1873..2835
                     /locus_tag="VGQ51_14500"
     CDS             1873..2835
                     /locus_tag="VGQ51_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV9037841.1"
                     /translation="MKIAQAILREELLVFDKTFKEAVKSQTPLLDRIMRFIVNRKGKQ
                     LRPMFVLLSAKLCGPVDDAAYRAASLVELLHTATLVHDDVVDESLERRGFFSTYALWK
                     TKISVLVGDYLLSKGLLLSLDHGDFRVLQLLSDAVREMSEGELLQIEKSRSLNLSESV
                     YFDIIRSKTASLLASSCAAGAYAASHDEVLTEKMRQFGEKVGIAFQIKDDLFDYGTED
                     VGKPTGNDIKEKKLTLPLIYTLNHLDSASRREIIYILRNKNKDQTKVQYVIRKVVEAG
                     GIEYAVGRMNAYRDEALNILHEFEDGEVRRGLEELVLYTTDRKY"
     gene            2860..>3913
                     /locus_tag="VGQ51_14505"
     CDS             2860..>3913
                     /locus_tag="VGQ51_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV9037842.1"
                     /translation="MDKRWNILQADTGKTAALQADLKVNGILCRILVQRGIETFGQAK
                     DFFRPLLSDLHDPWLMKDMDKAVDRIVRALSEGEKILIYGDYDVDGTTAVACLYQFLR
                     EHCPEDRLDFYIPHRYKEGYGISAMGIDHAQAGGYTLVISLDCGIKSAGLIRDARARG
                     IDFIVCDHHLPDPELPPAVAILNPKQLDCPYPYKELCGCGVGFKLITALCQRLELPGE
                     TALQYLDLVATAIAADIVPVDGENRILSWFGLKKVNENPNNGIGALIHASGLQKELKM
                     VNLVFMIAPRVNAAGRMDHARKVVHLFTTPHFDEALSYASQLHADNTDRKEHDLQTTE
                     EALTLLENDPGHKDRRS"
BASE COUNT          966 a         1025 c          986 g          936 t
ORIGIN      
        1 tagttaataa ctgcgttcac gcggtcccag ctcagctgtt gagcagactt gctcgattcg
       61 ccatagccta taacagccac cttacaggtg ggattgtcgc gcattttctg tgctgcactg
      121 gccaaaaggg ctttttgctc cttggtcagt gtaacagaac gacccttgaa cgtaatgctg
      181 ggcagatcgc cgatgctgca tttgcctttg ccgttaccat atacgccggc attcagcgaa
      241 tccctcaggt ctttacagca ttggggatcc ggacatttgc ctacgccatc ggcgtctaca
      301 ggctgacact gggtgggagt gatcagctcc ttatccttac aatccggtac gccgtcaccg
      361 tccgtatcac ggcttacacc atgtgtatcg accgggcagc ctttgggcgt attgggttcc
      421 aggtcaaact gatccgtaac accgtcccca tcggtatcat ccaacacggg tttgggcagc
      481 ttcatatggc ggggagcgtt gatctcgtta tacgcataat ccagcgggtt gatccaccac
      541 agcggctcaa cggcatgttt gcggaatatg ttcacgttga tgcccaacga tacgaaatta
      601 taacggtcct tggcgctcgt ttgagctacg ctgttcgttg cgcccggcgt ccagttgttc
      661 tgccattgct gaccgtccag caggttggta gccgtaaacg tgaatttatc ttccagcgcg
      721 atattcacct tatcggtaag cttaacctgt atgcccgcac cgactactcc gaccacggtt
      781 agcggtaccc ctcccagaag cgcgctcgtg ctcggacgct ccgcgatatg gttccatttg
      841 ccggtcagga cgttcttgag atcgctgata atatccttac gatgtttgta atccagcagc
      901 ggatatttat tgatcacgtt ctgataaccg gccaggtagc tgtcatcctg tttgtacagg
      961 gtcccgtagg tcatcaaacc ggggccaccg aagaaatatg cattccagcc gatcttggcc
     1021 ttatggaaat tgatattgtt gatcgtgaag atcccctgga aggaaagttc gtggaccgta
     1081 ctacggtagt tgtagaaaat gggataatag ctgccgctgt tctgttcagg gaacaggttg
     1141 gcctgcgtga gcgacgggtt gcggccgtaa gattgcgaat actgatagtt cagacctctg
     1201 agccggatcc aatcatattc ggcccggacg gaaaacatat aacccagcgc cttccggata
     1261 tgtatgccga agccgcctgt cggtccccag ttatgtactg aagtaaatcc attggtatat
     1321 cctcctttga caccgatctc ccattcattt cttggtttgg caggaaaagg ataagaattg
     1381 ttcaggaatt cattgtgctg tgcaagtcgt ttcgtaggta ttagagatga gtcccgaata
     1441 tcatagctgt agcccaccga ttgggcctgc gagtatgaca tcataaggca tagtaaccca
     1501 aataaaagag tcaatttttt acttgccata actgaagttt atgtgaatga atgaattaaa
     1561 cctgagaaga aatatggtgc aaaaatagat attggtgagc aataaccaaa tttttttaac
     1621 aatacgtatg aaataaaagt tgtataggcg cacaccgtta gatcgccaaa atttaaaaat
     1681 ataaccattc ctccgaaagt atattcaaag tagtaatatt gtccttccgt tcatattctg
     1741 cgggtccgtt ttgtataaca ttttcattac catgcaggag ccgtcgaaaa cggctcttag
     1801 gtctgacatt agccatttgc gtgaataggg ctaattttag cggaagccct attcacgcac
     1861 atatacctat aaatgaaaat agcgcaagcc atactacgcg aggagctgct cgttttcgat
     1921 aaaacgttca aagaggcggt caaaagtcag acgcctttgc tggataggat catgcggttc
     1981 atcgtcaata gaaaaggaaa gcagcttcga cccatgttcg tgctgctgtc cgccaaatta
     2041 tgcggccctg tcgatgatgc cgcctaccgg gcggcttcgc tggtcgagtt gctgcacact
     2101 gcaacgctcg tgcatgacga cgtcgtcgac gagtcgttgg aaagaagggg cttcttttct
     2161 acgtacgccc tctggaaaac gaaaatatcg gtgctcgtgg gcgattacct gttgtcgaaa
     2221 gggctgctgt tgtcgttgga tcacggcgat ttccgggtat tgcaattgct ttcggacgct
     2281 gttcgcgaaa tgagcgaggg cgagctcctc cagatagaaa agtccaggtc cctcaacctc
     2341 agcgagtcgg tatatttcga tatcatccgg agcaagaccg cgtcgctact cgcatcctcc
     2401 tgcgccgccg gcgcttatgc ggcctcgcat gatgaagtgc tcaccgaaaa aatgcggcaa
     2461 ttcggtgaaa aagtaggcat cgcgttccag atcaaagatg atcttttcga ctacgggacc
     2521 gaagacgtcg gaaaacccac cggcaatgac ataaaagaaa agaaactgac gctcccgctg
     2581 atctataccc ttaatcatct ggacagcgcc agccgcaggg agatcatcta tatcctccgg
     2641 aacaagaaca aggatcagac aaaagttcag tatgtcatca gaaaggtggt cgaagccggg
     2701 ggcatcgaat atgccgtcgg acgtatgaac gcctaccggg atgaagccct gaatatcctc
     2761 cacgaattcg aagacggcga ggtccgcagg gggctcgagg aactcgtcct gtataccacc
     2821 gatagaaaat attaataccc aactactccg tagcaaccga tggacaaacg ctggaacata
     2881 ttacaggccg ataccggaaa gaccgcagcc ttgcaggccg acctcaaggt caacggcatc
     2941 ctctgccgga tattggtaca gcggggcatc gaaactttcg gacaggccaa agattttttt
     3001 cgcccccttc tcagcgatct ccacgatccc tggctgatga aagacatgga taaagccgtc
     3061 gaccgcatcg tccgcgccct ctccgaagga gaaaagatcc tcatttatgg agactatgac
     3121 gtcgacggaa cgactgccgt tgcctgtctc tatcaatttc tccgggagca ctgcccggag
     3181 gaccggctcg atttctacat cccgcaccgg tataaagaag gatatggcat ttcagccatg
     3241 ggcatcgatc atgctcaggc cgggggctat accctcgtca tctcgctgga ctgcggcatc
     3301 aaatccgccg gtctcatccg ggatgcccgt gcacggggca tcgatttcat cgtttgcgat
     3361 caccaccttc cggatcccga attgccgccg gcggtcgcca tcctcaatcc caaacaattg
     3421 gattgcccat acccttataa ggaattgtgc ggatgcggcg tggggttcaa gcttatcacg
     3481 gctctttgcc agcgtctgga gctgcccggc gagactgccc tgcaatacct cgatcttgtg
     3541 gccacggcca tcgcggcgga tatcgttccc gtggacggcg aaaacaggat actctcctgg
     3601 tttgggttga aaaaggtaaa tgaaaaccct aacaacggga tcggtgcgct gatacatgcc
     3661 agcggtctgc aaaaagagtt gaagatggtc aatctggtct tcatgatcgc cccccgggta
     3721 aatgccgcag gccggatgga ccatgcgcgg aaggtggtcc acctgttcac taccccccat
     3781 ttcgatgagg ccctctctta tgccagccaa ttgcacgccg ataatacgga ccggaaagaa
     3841 cacgacctcc aaactaccga ggaagcgctg acgctcctgg aaaacgatcc cggacacaaa
     3901 gaccgccggt cta
//
LOCUS       DASYSV010000472         8951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_479400, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000472 DASYSV010000000
VERSION     DASYSV010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479400"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGQ51_14510"
     CDS             <1..822
                     /locus_tag="VGQ51_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037843.1"
                     /translation="VIRAGARGFAGFLARLMYQSSLQMNQKQEAAGLIDYNPFKAPLN
                     DTALITRTLILVNTFSFNRSDPHWGFDLSNTQNGGKTLLSYGYETRQTNEWSLRTRLN
                     LNKSVALTGVYRQGINQLNNSSSNFDSSNYDLRQFVLEPGITYTKGSNLRIGLSFRMT
                     DKQNSLRWGGQHYTSDAFNSEFKYNILQSTSIQGKFTMTSIVYTVGKNGSASTSSPVS
                     YTILEGLAPGKNYLWGLDLTRKLGGSLELSLQYEGRKAGETGVVHTGRAALRALL"
     gene            complement(980..1960)
                     /locus_tag="VGQ51_14515"
     CDS             complement(980..1960)
                     /locus_tag="VGQ51_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037844.1"
                     /translation="MSTTTNYPSASPQSQPPKKDYKNLVIGLLAAGFLGTWGYLLWNN
                     NKQEQVQTQQQSQIAKVTDEKGQLQKNFDDALVRLDSLTGTNNKLTATINEKDGQVSN
                     LKKQIRGLLSKNHMTEAEKKKAEDLIKQLNDKVSGLEQQVAQLTQDNQTLTTNNQQLT
                     SDKEKLTNDLQTSTATNQDLTKKVDVASTLNASNISVTPMHEKKDGQDKETTTARKVN
                     KLVIAFDVANRIAQTGPTEVYVCITGPDGKPISVPALGSGTFTTREEGDKVFTAKVPV
                     EFEAGKTKSVQFAWKQNSEFQRGNYKIEIYHNGFKIGEGTRELKKGGIFG"
     gene            2127..3125
                     /gene="holA"
                     /locus_tag="VGQ51_14520"
     CDS             2127..3125
                     /gene="holA"
                     /locus_tag="VGQ51_14520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV9037845.1"
                     /translation="MSVEKILQDWKKLQFKPVYWLEGEEEYYIDQLVDYAESRILSPE
                     EAGFNLTVFYGKDADWTAVINACRRYPMFAERQVVILKEAQQMRDIDKLEPYIDKPLA
                     STVLVVAYKEKKVDGRSRLAKLLKEKAELFSTKKLYENQLPEWANGLIRSKGYEITQK
                     ALLLLVDHIGNDLSRINNEIDKLLVNLAGSKRISEDDIERYVGVSKEYNVFELQDAFA
                     KKDKVKAVRIIQYFEGNPKAGPIQLILPSIYNFFSKVYMIFGQPARDEKGVASAIGVN
                     PFFVKDYLAAVRNYGYEGIENGLLLLHAYNLKSVGVGSAGTEDASLLKELVVKIMD"
     gene            complement(3154..4134)
                     /locus_tag="VGQ51_14525"
     CDS             complement(3154..4134)
                     /locus_tag="VGQ51_14525"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV9037846.1"
                     /translation="MRIHYHTDQLPSFRRAVVTIGTFDGVHTGHARILQQLREEAARI
                     DGETVIITFFPHPRKIVKGGSGDIRLINTLEEKIELLSWQRIDHLVIVPFTEAFSQMT
                     AEEYIQDFLLARFHPHTVIIGYDHRFGKGRLGDYHLLEQCSGSGQFRLEEIPVHLLDE
                     VSVSSTRIREAILQADIDTANQLLGYPFFFSGTVVKGRQLGRTLGFPTANLSTDNPEK
                     LTPADGIYAVEAVLLPESPLFGSPRMKGMMSIGMRPTVDGRNRTIEVNLFDFDEDIYG
                     KELRVFVIRYLRPELKFNGLEQLKTAIAKDKADALAVLPSDRPRPSPSDR"
     gene            4206..5423
                     /locus_tag="VGQ51_14530"
     CDS             4206..5423
                     /locus_tag="VGQ51_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEV9037847.1"
                     /translation="MSLYARRGVSAQKEEVHQATENLDKGLFPNAFCKIYPDYLGGDD
                     GWVNLMHADGAGTKSILAYLYWKETGDVSVWKGIARDAIAMNLDDLLCVGIYDNLLFS
                     STIDRNRRVIPGEVLSAIIEGSQEFFDELASYGVGIRYLGGETADVGDVVRTIAVNGT
                     MTARWPKDRLVTNDKIRAGDVIVGFASSGKAAYEQEYNSGIGSNGLTSARHDLLHKTY
                     GARFHESYDTMLEDAVVYIGPHRMTDPIEIPAFSGAGEISGGSKTEGVVSTDIGHLLL
                     SPTRTYAPLVRRLLTEQFDGLHGLIHCSGGGQTKCMKYLPGAFRIVKDQLFKPPVIFQ
                     LIQSASGSDDREMYQVFNMGHRLEAYVEEATAASLIAIAGELGIEAQVVGRVEAATKK
                     SLVLNVKGNEVVY"
     gene            5834..6691
                     /locus_tag="VGQ51_14535"
     CDS             5834..6691
                     /locus_tag="VGQ51_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEV9037848.1"
                     /translation="MRQLKIATQITNRDSQAVEKYLQEISKIPMITPEEETVLAQRIK
                     MGDQKALDKLVQANLRFVVSVAKQYQHQGLSLSDLINEGNLGLIKAAQRFDETKGFKF
                     ISYAVWWIRQSILQALAEQGRLVRLPQNKIGTYNKANKAYMAFEQEHEREPSTEELAE
                     LLEMSETEINNIFQSNTRHSSLDAPVHEAEDVAMGDLLEGGDETDDDVMKDSLRAEIR
                     RVLKSLSPREAEIVNAYFGLDGENGVTIEQIGQKYDLTKERIRQIKERAIKRLQKARY
                     SNALKAYLG"
     gene            complement(6741..8327)
                     /locus_tag="VGQ51_14540"
     CDS             complement(6741..8327)
                     /locus_tag="VGQ51_14540"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HEV9037849.1"
                     /translation="MSLFFRWLLFSLIPLFSIAQNGRSLQTGSWGDQGNGTFINPVLY
                     ADYSDPEVIRVGDKYFMVCSEFANMGMPVLESDDMVNWTIIGQVFSRLNFPEYDHFQR
                     YGRGSWAPALRFHDNRFYLYFCTPDEGLFMSTATDPAGPWASLIQVHAGPRWEDPCPF
                     WDEHDGQAYLGHSRTGAGPIIIHKMSADGTTLMDSGVTVYTGPTAEGTKIFQLDGYYY
                     ISIPEGGVSKGWQTVLRSKNIYGPYEKKVVLERGTTGINGPHQGALVATPAGQWWFYH
                     FQSDGAMGRVLHLEPVLWQDGWPLIGADIDHNGIGEPVYVWKKPDIAGEHPIRAPQTD
                     DEFSGSRLGLQWESNHNPVPSAWSLTQKPGWLCLNALQADSFFHARNTFTQKIMGTTG
                     TVTVRLDPAGMADGQQAGLCAMSSIYALVGLRKKEGHLWLFFDDGKSPQELQLRSGIV
                     YLKMQLRIKEGPNQCLYSTDGKTWEPVGSPFETKFGFWKGTRIGLFSYNTLRDGGTAA
                     FDWFHYDYDGPKAAAPVAGR"
     gene            8458..>8951
                     /locus_tag="VGQ51_14545"
     CDS             8458..>8951
                     /locus_tag="VGQ51_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9037850.1"
                     /translation="MEQNAHEKVHCLIIGSGPAGYTAAVYAARANLKPVLYQGIQPGG
                     QLTITTEVENYPGYPDGVQGPEMMVHFEKQATRMGADIRYGLATKVDFSTHPLKVWID
                     EEKLIEADAVIIATGASAKWLGLESEQRLNGYGVSACAVCDGFFFRNKEVAIIGAGDT
                     AAEEA"
BASE COUNT         2111 a         2276 c         2521 g         2043 t
ORIGIN      
        1 gtgatccgcg ccggggccag gggattcgcc ggttttctgg cccgcctgat gtatcagtcc
       61 tcgctgcaga tgaatcagaa acaggaggcg gcgggtctca tcgattacaa ccctttcaag
      121 gccccgctga atgatacggc actgatcacc cgaacgctta tcctggtcaa tacgttttcg
      181 ttcaatcgca gcgacccgca ttggggattc gatctcagta atacgcagaa tggcggaaag
      241 accctgttga gctatggcta tgagacccgt cagaccaatg aatggtcgtt aaggacccgt
      301 ctcaatctca ataaatctgt agcgcttacg ggcgtctacc ggcaggggat caaccagctc
      361 aacaattcca gttccaattt cgatagcagc aattacgatc tgagacagtt tgtgctggaa
      421 cccgggatca cgtatacgaa gggctccaat ctgaggatcg ggcttagctt ccggatgacc
      481 gacaaacaga attcattgcg atggggcggc cagcattata cttccgatgc ctttaattcg
      541 gaattcaaat acaatatcct ccagagcact tcgatccagg ggaagtttac gatgacgtcg
      601 atcgtctata cggtgggtaa gaatggttcg gccagcacca gctcgcctgt cagctatact
      661 atcctggaag gactggcgcc gggaaagaac tatttgtggg gactcgatct caccaggaag
      721 ctgggaggaa gcctggagct gagtctccag tatgaggggc gcaaggcagg ggagacggga
      781 gtcgttcata cgggacgtgc ggcgctgcgc gcgttactgt gacaagggtg ctctggcaaa
      841 cgaagacccc catcaataaa tgaaaggcgc ccgaactcag gacggctcgg agagccgtcg
      901 gccgaaggcc aatcataaaa ctaaaggcgc ccgagcttta gctcgggcgc ccttgtcttg
      961 tcctttatat agaccggaat taaccgaaaa taccgccttt cttgagttcc cgggtgcctt
     1021 cgccgatctt gaatccattg tgatagatct cgatcttgta gttacccctc tggaattcgc
     1081 tgttttgttt ccaggcgaac tgtacggatt tagtcttgcc ggcttcgaat tcaaccggga
     1141 ctttagccgt gaataccttg tcgccttctt cacgggtggt gaaggtgccg gaaccgagcg
     1201 caggaaccga gatgggcttg ccatcggggc cggtgatgca tacatatact tcggtcgggc
     1261 cggtttgcgc gatacggttg gcgacgtcga aggcgatcac cagcttgttc actttacgtg
     1321 cggtggtggt ttccttgtcc tgcccgtctt tcttttcgtg catcggagtg actgaaatat
     1381 tggaagcatt gagcgtagaa gcgacgtcta ctttcttggt gagatcctgg ttggttgccg
     1441 tgctggtctg caggtcattg gtcagttttt ctttgtccga cgtcaactgc tgattgttgg
     1501 tggtcagcgt ctggttatcc tgcgtcagct gggcgacctg ttgttcgaga ccgctgactt
     1561 tgtcattaag ttgcttgatc aggtcttcgg cctttttctt ttcggcctcg gtcatgtgat
     1621 ttttgctcag cagaccgcgg atctgtttct tgaggttaga aacctggcca tccttttcat
     1681 tgatggtagc ggtgagtttg ttgttggtgc cggtgagaga gtcgagccgt accagcgcgt
     1741 cgtcgaagtt cttctggagc tggccttttt cgtcggtgac cttggcgatc tgcgactgtt
     1801 gctgggtctg aacctgttct tgtttgttgt tattccagag caaataaccc caggtgccga
     1861 ggaagccggc tgccagcagt ccgataacta aattcttgta gtcctttttc ggcggttgac
     1921 tttggggaga agctgaggga tagttggttg tcgtactcat agcttatgtt ttttcaaaag
     1981 ttatagataa tatttcatcc aatcctgtaa cccaatttcg ggttacaaga ccatgctggt
     2041 ctaaaaccgt gccaaactca atttattgtg tagatcacct gattcctaaa gaattatgta
     2101 atattgccgc aataaaagtg ttttgaatgt ccgtcgagaa gatattgcag gactggaaaa
     2161 agttgcagtt caagcccgtg tactggttgg agggagaaga ggagtattat atagaccagc
     2221 tggtggatta cgcggaaagc cgcatccttt cgccggagga agcgggtttc aatttaactg
     2281 ttttttatgg gaaggatgcc gattggacgg cggtgatcaa cgcctgccgg cgttatccga
     2341 tgttcgccga gcggcaggtg gtgatcctga aagaggcgca acagatgcgc gatatcgaca
     2401 agctggagcc ctatatcgac aaacctttgg cttcaaccgt gctggtagtc gcctataagg
     2461 aaaagaaggt ggacgggcgg agccggctgg ccaaattgct gaaagaaaag gccgaactgt
     2521 tcagcaccaa aaagctatac gagaaccagc ttccggaatg ggccaatggg cttatccgga
     2581 gcaaaggata cgagatcacc caaaaggcgt tgctgttgct ggtcgaccat atcggcaacg
     2641 atctgagccg catcaacaac gagatcgaca agctgctggt gaacctggct ggcagcaagc
     2701 gcatcagcga agacgatata gagcggtatg taggggtcag caaggaatac aatgtattcg
     2761 agctgcagga tgcattcgcc aaaaaggaca aggtaaaagc cgtccgtatc atccagtatt
     2821 tcgaaggcaa tccgaaggcc ggacctattc agctgatatt gccttccatc tacaatttct
     2881 tcagcaaggt gtatatgatc ttcggccagc cggcacgcga tgaaaaaggc gtcgcatcgg
     2941 ccatcggcgt gaacccgttt tttgtcaaag actatctggc cgcggtgcgc aattatggtt
     3001 atgaagggat cgagaacggg ctgctcctcc tgcatgccta caatctgaaa agtgtagggg
     3061 tgggcagcgc gggcacggag gatgcgtcgc tgctgaagga gctggtcgta aagatcatgg
     3121 attagtcgga cgcgcgccca aatcagaggt gcgctaacgg tccgatggag aggggcgcgg
     3181 ccggtcagac gggagaacgg caagcgcgtc tgccttgtct ttggcgatgg ccgttttcag
     3241 ctgttcgagg ccgttgaact tcaattcggg ccgcaggtac cggatcacga agacccgcaa
     3301 ttcctttcca tagatgtcct cgtcgaaatc gaagagattc acttcgatgg tccggttcct
     3361 accgtctacg gtcgggcgca tcccgatgct catcattcct ttcatacggg gcgacccaaa
     3421 aagcggggat tcgggaagca ataccgcctc cactgcgtag atgccgtcgg cgggggtcag
     3481 cttttcggga ttgtcggtcg aaagattggc cgtcgggaat ccaagcgtcc ttcccagctg
     3541 gcgccctttt acgaccgtgc cggaaaagaa aaaaggatag ccgaggagtt gattggccgt
     3601 atcgatatcc gcctgtaaga tcgcttcccg gatcctcgta gagctgacag atacttcatc
     3661 tagcaggtgt accggtatct cttccagccg gaactgtcct gacccgctac actgttcgag
     3721 caggtggtag tctcccagcc gtcctttccc gaaacgatga tcatagccga tgatcacggt
     3781 atgcggatga aaccgggcca gcagaaaatc ctgaatatat tcttcggcgg tcatttgcga
     3841 gaaggcctcg gtgaaaggga cgatcaccag gtgatcgatc cgctgccagg aaagcagttc
     3901 tatcttctcc tcgagcgtat tgatgagccg gatatcgcct gagccgccct ttacgatctt
     3961 gcggggatga ggaaaaaagg tgatgatcac cgtttccccg tcgatgcggg ccgcctcttc
     4021 tctcagctgt tgcaggatac gcgcatgacc cgtatgtacg ccgtcaaatg taccgatcgt
     4081 gaccactgcc cgacggaagg acggcagttg gtcggtatgg taatggattc gcatgtgaaa
     4141 ctaagggcgg cccggagggc cgccacggca aatctaaacg atttatccgt tacctttgct
     4201 gccttatgtc attatatgcc cgacgcggcg tctccgctca gaaggaagaa gttcatcagg
     4261 ctaccgaaaa cctggacaag ggtctcttcc cgaacgcgtt ttgcaagatc tatcccgact
     4321 atttaggggg cgacgacggt tgggtcaacc tcatgcacgc cgatggggcg ggaaccaaaa
     4381 gcatcctggc ctatctgtat tggaaggaaa caggagacgt ttccgtatgg aagggtatcg
     4441 cgcgcgacgc tatcgcgatg aacctcgacg atctgttgtg tgtgggtatc tatgacaacc
     4501 tgctcttctc ttccaccatc gaccgtaacc gccgggtgat accgggggaa gtgctttcgg
     4561 ccattatcga aggctcacag gaatttttcg atgagctggc gtcgtatggc gtcggcatcc
     4621 gttatctggg tggcgagacg gccgacgtag gggacgtcgt ccggactatt gcagtcaacg
     4681 gaacgatgac ggcccgctgg ccaaaagacc ggcttgtcac caacgacaag atccgcgccg
     4741 gcgacgtgat cgtgggtttt gccagttcag gtaaggctgc atacgaacag gaatacaaca
     4801 gcggtatcgg cagcaacggg ctcaccagcg cccgtcacga tctgttgcac aaaacatatg
     4861 gcgccagatt ccacgagtct tatgacacca tgctcgaaga cgcggtagtc tatatcggac
     4921 ctcaccggat gacggacccg atcgaaatcc ccgctttctc cggcgccggc gaaatttccg
     4981 gaggatcgaa gacggagggg gtcgtatcca cggacatcgg gcatctcttg ttaagtccta
     5041 cccgaaccta tgctccgctg gtccggagac tgctgaccga acagttcgac ggtctgcacg
     5101 ggctcatcca ttgcagcgga ggagggcaga ccaagtgtat gaaatatctg ccgggggcgt
     5161 ttcgtatcgt gaaggatcaa ttgttcaaac cacccgtcat tttccagctg atacaatctg
     5221 ccagcggctc cgatgacagg gaaatgtacc aggtatttaa tatgggtcac cggctggagg
     5281 cgtacgtgga ggaagcgact gccgcatcgc tgatcgcgat tgcgggtgag ctcgggatcg
     5341 aagcgcaggt cgtcggacgc gtcgaagctg cgaccaaaaa gagcctcgtg ttgaacgtca
     5401 agggcaatga ggttgtttac tgattaataa tcaataagtt aaagctatgt ttcgcgggtg
     5461 gccggaccac gtaccggggt cggacatccg aaaaaagctc atctgttaaa gcgacgctaa
     5521 aaattcgcgt cggagattag ctgcaaaagc cagcggagca ttgcaaagcg ccaaaatatt
     5581 ggatcaactc taaaaaaatg attaaaaaca agcattttta ttccacacta ctatgatttt
     5641 ccagttttgg tgactacttt cgctgacggt tctggacgga acgacatgaa ttttatttct
     5701 atatcttttt tgccccctcc gtgaacactc ccggctcagt aaatgtttgt tgattagtac
     5761 cccccaatgc aaaaccgtta aacggtagct tgtccaaacg agattttgtc atcttttatt
     5821 attctttttc gatatgaggc aactaaagat tgctacacag attacgaacc gtgattctca
     5881 ggcggttgaa aagtatttgc aggagatctc caagatcccg atgatcactc ccgaagagga
     5941 gactgtcttg gcgcaacgca tcaagatggg ggatcagaag gctctggaca agctggtgca
     6001 ggccaacctg cgctttgtgg tctccgttgc aaaacaatat cagcaccagg ggttatcatt
     6061 gagtgatttg atcaacgagg gcaacctcgg cttaattaag gcggctcaac gctttgatga
     6121 aaccaagggt ttcaagttca tttcatacgc ggtatggtgg atccgccagt ccatcctgca
     6181 ggcgttggca gagcagggca gattagtccg cctgcctcaa aacaaaattg gcacttacaa
     6241 caaggcaaac aaggcctaca tggctttcga acaggaacat gagcgggagc ctagcacgga
     6301 agagttagcc gagttgctgg aaatgagtga gacggagatc aacaacattt tccaaagtaa
     6361 tacccgtcac agcagtcttg acgccccggt tcacgaggca gaagacgtag cgatgggaga
     6421 tttgctggaa ggtggcgacg agacggacga cgacgtcatg aaagattctt tacgcgcgga
     6481 gatccgccgg gtgctgaaat ccctcagtcc ccgcgaagcg gagattgtca acgcctattt
     6541 tggcctggac ggcgaaaatg gggtaaccat cgagcagata ggccagaaat acgacctgac
     6601 gaaggagcgt atccgccaga tcaaagaaag ggcgatcaaa cgccttcaaa aggcgcgcta
     6661 cagtaacgcg ctgaaagcat acctgggtta ggcccagccg aaaacactca aaaatcccgg
     6721 cctctggccg ggatttttca ttagcgcccg gctactggtg cggcagcctt tggtccatca
     6781 tagtcgtaat ggaaccagtc gaaggctgcc gttccgccgt cgcggagcgt gttatagctg
     6841 aaaaggccga tccgggtccc tttccagaag ccgaatttgg tctcgaatgg cgagccgacc
     6901 ggctcccagg tctttccatc cgtactatag agacactgat tgggcccctc tttaatccga
     6961 agctgcattt tcaggtagac gatgccgctt cgaagctgca gttcctgcgg cgacttgcca
     7021 tcgtcgaaga acagccacag atggccttcc tttttccgaa gaccgacaag cgcatagatc
     7081 gaactcatcg cgcataaccc ggcctgctgc ccgtcggcca tgccggcggg atccagcctg
     7141 accgtgacgg tacccgtggt acccatgatc ttttgggtga aagtgttccg cgcatggaaa
     7201 aagctgtccg cctgcaaggc gttgagacag agccagcctg gtttttgggt cagtgaccat
     7261 gcggaaggca cgggattatg gttgctttcc cattgtaacc cgagccttga tccggagaat
     7321 tcatcatctg tctgaggcgc acgtatcgga tgctcgcccg cgatgtccgg ctttttccat
     7381 acatacaccg gctcgccgat gccattgtga tcgatatcgg cgccgatcag cggccagccg
     7441 tcctgccaga gcaccggctc aagatgcaac accctgccca tggctccgtc agactggaaa
     7501 tgatagaacc accactgccc ggcgggcgtg gctaccagcg ctccctggtg cggaccgttg
     7561 atacccgtgg tcccccgttc cagcacgacc ttcttttcat agggaccgta gatgttcttc
     7621 gatctcagga cagtctgcca gcctttgctg acgccgcctt cggggatgct gatataataa
     7681 taaccgtcga gctggaagat cttcgtaccc tcggcagtgg ggccggtata gacagtgacg
     7741 ccggagtcca tcagggtcgt tccatcggcg ctcattttat gaatgatgat gggtccggcg
     7801 ccggtgcggc tatgtcccag ataggcctgg ccgtcatgtt cgtcccaaaa gggacaggga
     7861 tcttcccacc gcggccccgc atgcacctgt atcaaagacg cccaggggcc ggccggatcg
     7921 gtagcggtac tcatgaacaa gccttcatcg ggggtacaga aatacaaata aaaccgattg
     7981 tcgtgaaacc ggagggcggg agcccacgag ccgcggccat aacgctggaa atggtcatat
     8041 tcgggaaagt tcagccggga aaacacctgc ccgatgatcg tccagttgac catatcgtcc
     8101 gactccaata ccggcatgcc catgtttgcg aattcggagc ataccataaa gtatttgtcc
     8161 ccgacccgga tgacctcagg atcggaatag tcggcataaa gcacaggatt gataaaggtg
     8221 ccattgccct ggtcgcccca ggagccggtt tgaagcgatc tgccgttctg cgcgatggaa
     8281 aaaaggggga tcagcgaaaa aagcagccat cggaaaaata gagacatcgg aatagcagtt
     8341 tgttgataac caggtgtgaa actaagtcat tcaaccgaca cttggctatc cattttcggg
     8401 ttaaatttgc aggcttaaac cgcggataac agccgcggtg accatataat ctatcgcatg
     8461 gaacagaacg cccacgaaaa agtacattgt ctgatcatcg gttcgggtcc ggccgggtat
     8521 acggccgccg tttatgcggc gcgcgccaat ctgaaacccg tcctttacca gggtattcag
     8581 ccgggaggac aattgacgat aacgacggag gtggagaact atcccggcta cccggacgga
     8641 gtgcagggac ccgagatgat ggtacatttt gaaaaacagg cgacccggat gggcgcggat
     8701 atccggtacg gcctggcgac aaaagtggat ttttccacac atccgctgaa ggtctggatc
     8761 gacgaggaaa agttgatcga agccgacgcg gtgatcatcg ccacgggagc atccgccaaa
     8821 tggctgggtc tggaatccga acagcggctc aacggttatg gtgtcagcgc ctgtgccgtc
     8881 tgtgacggtt tctttttccg caacaaggag gtggccatta taggcgccgg cgatacagcg
     8941 gccgaagaag c
//
LOCUS       DASYSV010000473        10937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_352309, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000473 DASYSV010000000
VERSION     DASYSV010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10937
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352309"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="VGQ51_14550"
     CDS             complement(<1..1436)
                     /locus_tag="VGQ51_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037851.1"
                     /translation="MSVKLKPAGKFLVIAIIVTGGILSVKWYQGRPKDAGKAVEVGKV
                     ALPDAPEASLNAHAILLPLPSSTEAVNGGTRITWERMAWNSQFSGMYANGGVRTTKGS
                     LFDKANLDITYIRQDDCNKQMADLVKFAGEFRTNPNTPGVLITFMGDGMPAFMTALSK
                     ELEPLGPEYQPIILPIAHGKSYGEDQIMGPPSWKQDPHNAVGKCVAGVLRDGDVNILL
                     KWAGDNGIKVNPDETTYDADAINLIAASDFLDAPTKYISGYTESRKIIVNGRTTGRDT
                     ALGVDAVATWTPGDVNVATKKGGLVTIASTRQYASQMPNQTIAIKKWAYDHRTDIENM
                     IIALAQAGDQVKSFTEAKKFAAQVSTAVYQEQNADYWLKYYNGVEEQDIQGMKVNLGG
                     SAVFNLADMANTYGLGSDKVDRYKAVYNTFGNLMVKMYPALMPSFIPYEKAVDKSFLL
                     SVISNHPDLLEGKALETQYASQITARVSS"
     gene            complement(1467..2459)
                     /locus_tag="VGQ51_14555"
     CDS             complement(1467..2459)
                     /locus_tag="VGQ51_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037852.1"
                     /translation="MNTLIQKTKTPGWFSEPKNVATTVVGLALLAGLTYGFMTYVLPW
                     LVKVTWNLVNLVIGGIVCGFLLLTISNRKFWRALKYLSEFVANYSIGLAIELNPFSIL
                     QTKIEQGYKDRNILYKQSARLKGKQSELMDKLGEKEKELQLNIQKVKILQSESGSSRQ
                     VSLYTNNVMRCREYIDNVTPIVGDLNKLIKFADAAYEESGIMLEDAKLDLEAKKDLYY
                     SVTTGLSAVTSAMKAFKGDDELNRDAEKALALLKVRIGEKIGHIKSAIDVTSRFMDDQ
                     VLEDKAKSAQAIDLINRFDLGKDFHYAGSALAGSGDKGRLKDVTVPDRFKGLLD"
     gene            complement(2477..3118)
                     /locus_tag="VGQ51_14560"
     CDS             complement(2477..3118)
                     /locus_tag="VGQ51_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037853.1"
                     /translation="MANIGKRILSVFVDNGDEQPNYRAEETANTAAAAASLPNQPPLA
                     GSAGDGPGDQRFTAHFDKLFTDANIPGPDYYEFARMIVAMQVIPDERSRYVAAFAGLQ
                     AQGLDKDKLLSTAGEYLRVLGEDAGSFQKTVDSALQEKVQNRSAEAEEKNRRIQELSQ
                     QILELQKQIGTMQKDIRENQEKLEGSRTAYTAECERRRQQIEKDIEKINHYLS"
     gene            complement(3151..3330)
                     /locus_tag="VGQ51_14565"
     CDS             complement(3151..3330)
                     /locus_tag="VGQ51_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037854.1"
                     /translation="MKKLIGVAAILFTLLISIIYRQLTARPAKDRPAKACIRAKNCVR
                     SGWREGMMLSRLLLQ"
     gene            4274..5278
                     /locus_tag="VGQ51_14570"
     CDS             4274..5278
                     /locus_tag="VGQ51_14570"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEV9037855.1"
                     /translation="MSKRTDFVYSVDVEPHRIRTRQILKQHPEMRKLIGKNKYTIFAV
                     LGLVLFQVLLAWLVKDRSWWIIVGAAYLLGAFADHSLFVMIHECAHHLLFKNRAANRL
                     AGILANLPQLFPSSVSFERYHIKHHSFQGIHELDADLPNRWEARLINNFFIGKVIWLL
                     FYPLFQVFRISRIREVKPFDKWVALNWVAEAAFITGIWIAFGPKAIAYLLLSFFFSVG
                     LHPLGARWVQEHYLTHDDQQETFSYYGVLNTVSFNVGYHNEHHDFPSIPWNRLPLIKK
                     TAPGFYDNLQSHKSWTLLFFRFLFDKEISLFSRVVRKDRGKVALTDRSIPDRELVEQA
                     "
     gene            complement(5281..6159)
                     /locus_tag="VGQ51_14575"
     CDS             complement(5281..6159)
                     /locus_tag="VGQ51_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9037856.1"
                     /translation="MSTNIIREITPLTQNDCFTIFSRVKKEFTFPLHYHDELELNLIL
                     NAKGARRIVGDHIDTIDDVELVLVGPNLYHAWFTHQCKSEEIKEVTIQWHKDLFEEKL
                     LRRNQLAFIRNMMDRSQKGILFSRETTAGLVPRILSLNQKNGFDSVLELLSILHDLST
                     SRNMRTLSDASFTNQHFTYNSRRIEKAFEYMNNNYDKSITLGEVARLVSMTEVSFSRF
                     IKKRTGNTFIDSLNEIRLGHASRMLIDTTHSISEISYHCGFNNISNFNRIFKKKKTCT
                     PKEFRESFNSGTRVFI"
     gene            6422..8275
                     /locus_tag="VGQ51_14580"
     CDS             6422..8275
                     /locus_tag="VGQ51_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEV9037857.1"
                     /translation="MKLQLPDLAIIIAYLVTMVVIGIRLRKKASKNKVNYLLGGKSLP
                     WYMLGLSDASDMFDISGTMWMVSLCFVYGMKSIWIPWLWPVFNQVFLMVYLARWLRRS
                     NATTGAEWMATRFGQTGPWVRSSHNIVVAFALLSCLGFLAYGFVGLGKFMQIFIPWEA
                     VRQYIPFHVSPAYVPDLYGIVFTLFAVFYAILGGMHSIVLGDVIKYAIMTVACISVAI
                     IAMLRLHEHANGLPIPKGWLNPFFGWRLDLHWKGIVEDVGGKISSDGFSPFGLFFMMM
                     LFKGIAASLAGPAPNYDMQKVLSTRSPGEASKMSGFVSIILLPIRYSMIIGLTVLGLI
                     YFNNISGELATPGGTDFEKILPATINAFLPVGLMGLVLTGLLGAFMGTFAGTLNAAQA
                     YFVNDVYLKYINPSASNRQTISMNYLVGIVVVVIGIGLGLFVEDVNTILQWIVSGLWG
                     GYAAANLLKWHWWRFNAGGYFWGMLGGILPAIALAFLKYKGVLHGLDLYYWPVIFLLS
                     VAGSIIGSYSCPPTDPEVLKSFYRTVRPWGCWGTVHRLVLKEDPAFSGNRNFKRDMFN
                     VALGIIGQLCLTLLPMYIVLGQKLSLLLNVILLLAIALILKKTWWNRLKDY"
     gene            8299..9498
                     /locus_tag="VGQ51_14585"
     CDS             8299..9498
                     /locus_tag="VGQ51_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEV9037858.1"
                     /translation="MMNHHFKERMAQLNKQYSELIRRPNEPLVPGNGIFLRYKSPILT
                     AAHTPLFWRYDLDELSNPYLMERFGINGVFNAGAIKWKDRYALMVRVEGWDRKSFFAI
                     AESPNGIDNFRFRDSPIHMPGSGRPETNIYDVRLTRHEDGWIYGLFCTESKDPDAPVS
                     DQSAAVAQCGIARTLDLVDWERLDDLRTRSPQQRNVVLHPEFVDGKYAFYTRPQESFH
                     ATGTGGGVGFGLSDSITNALIDSETIIDPRLYHTINEAKNGLGPGPIRTRLGWLHLAH
                     GVRNTAAGLRYTLYLFMTDPNDLTKVIYKPGGYFIAPRGEERVGDVSNVVFCNGWIAD
                     DDGQVYIYYASSDTRMHVATTTIEQLLDYVVNTPEDEGSSASSVYSLQQLIDKNLAAR
                     EILRIFP"
     gene            9522..10205
                     /locus_tag="VGQ51_14590"
     CDS             9522..10205
                     /locus_tag="VGQ51_14590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV9037859.1"
                     /translation="MRSLSRIAILLSLFIGTGLLSSFHRKPKKVIFFGDSITEQGAKP
                     GGYIVKMTDAIAQKNLANDYSLIGAGVGGNKVYDLYLRMDDDVLSQNPDVVIIFIGVN
                     DVWHKTLLGTGTDIDKFEKFYIAIINELLDRHIRTILCTPAVIGEKTDFTNQQDGDMN
                     AYSQVIRNLAQKFHCDLIDLREIFHNYELKNNPANKASGILTVDKVHLNDAGNQLVAD
                     KMLEVLLSK"
     gene            10230..10922
                     /locus_tag="VGQ51_14595"
     CDS             10230..10922
                     /locus_tag="VGQ51_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV9037860.1"
                     /translation="MQKTILITGANGNLGAATVRRFLDRGYKVIAVDQSGTHLGFAAD
                     HDDFELRSVDAGNESVVGAFIEEIIELYGRIDGALLLVGGFAMGDIASTDSEALRKLY
                     ALNFETTWNFARPLLRHMMENDYGRIVFMGARPALIPDQGKHVLAYALSKSLLFELAR
                     LLNATAKGKNVVTSVIAPSTIDTPVNRQSMPDADPSAWVRPEEIAALMDFICSEEGKA
                     LREPVYKIYNNS"
BASE COUNT         2434 a         2893 c         2824 g         2786 t
ORIGIN      
        1 gaagacaccc gcgccgtgat ctgcgacgca tactgggttt cgagagcctt cccttccagc
       61 agatcggggt gattggagat cacactgagc aggaaagatt tgtccactgc cttttcatag
      121 ggaatgaagc tgggcatgag ggcggggtac atctttacca tcaggttgcc gaacgtattg
      181 tagacggctt tatagcggtc gaccttatcg ctgcccaacc cataggtatt ggccatgtcg
      241 gcgagattga acaccgcgga cccgcccagg ttcaccttca taccctgtat atcctgctct
      301 tcgacgccat tgtagtattt cagccagtaa tccgcgttct gctcctggta gaccgcggta
      361 ctgacctgcg cagcaaactt tttagcttcg gtgaaagatt tcacctggtc gccggcctgc
      421 gcgagggcga tgatcatgtt ctcgatatcg gtacggtgat cataggccca cttcttgatg
      481 gcaatcgtct ggttcggcat ctgcgacgca tattgccgtg tactggcaat ggtcaccaat
      541 ccgccctttt tggtcgccac attcacgtcg cccggcgtcc acgtcgcgac ggcatccaca
      601 ccaagcgcgg tgtctctccc cgtagtccgg ccgttgacga taatcttcct gctttccgta
      661 tagccgctta tatatttggt cggtgcatcc aaaaaatcgc tggcggcgat caggttgatc
      721 gcgtcggcgt cataggtggt ctcatccggg ttaaccttga tgccattgtc acctgcccat
      781 ttcagaagga tattgacgtc accgtcgcgg agcacaccgg ctacgcattt acccacggca
      841 ttatgagggt cctgtttcca cgagggcggt cccatgatct ggtcttcccc ataagattta
      901 ccatgtgcta tcggcagaat gatgggctga tattcgggac ccagaggttc cagctctttc
      961 gacagggccg tcataaaggc cggcatccca tcgcccataa aggtgatcag cacgcccggc
     1021 gtattcgggt tggtcctgaa ctcaccggcg aacttcacga gatcggccat ctgcttgttg
     1081 caatcgtcct gacggatata agtgatatcc aggttggcct tatcgaacag agatcccttc
     1141 gtggtccgta cgccgccatt ggcatacata cccgagaatt gagaattcca ggccatgcgt
     1201 tcccaggtga tcctggttcc cccattcacg gcttccgtgg aagacgggag gggtaaaaga
     1261 atggcgtgtg cattcaacga agcctcgggg gcatcgggaa gcgcgacctt gcctacttcc
     1321 acggccttgc ccgcatcttt gggccggccc tggtaccatt ttacggagag gatgcctccc
     1381 gttacgatga tggcgatgac taaaaatttt ccggcgggtt tcaatttgac tgacatatac
     1441 ttgaattttt aaggttaaaa aatggtttaa tccaataatc ccttgaagcg atcgggcacg
     1501 gtcacgtctt tcaaccttcc tttgtccccg cttccggcca gggccgaccc ggcgtaatgg
     1561 aaatccttgc ccagatcgaa ccggttgatg aggtcgatcg cctgcgcgga tttggccttg
     1621 tcttccaata cctggtcgtc catgaatcgg gaagtgacat cgatggccga tttaatgtgc
     1681 ccgatctttt cgccgatgcg gaccttcagc aacgccaagg ccttttcggc atcccggttc
     1741 agttcgtcgt cgcccttgaa ggccttcatc gcactcgtca ctgcggagag tccggtggtg
     1801 acggaataat agaggtcctt cttggcctcc agatcgagct ttgcgtcctc gagcatgatc
     1861 ccgctttctt cataagcggc gtcggcaaac tttatcagct tgttgaggtc gcctactatc
     1921 ggggtcacgt tatcgatata ctcacggcaa cgcatgacgt tgttggtata gaggctgacc
     1981 tggcgggaag agccgctctc ggattgcagg atcttcacct tttggatgtt cagctgcagc
     2041 tctttttctt tttcgccgag cttatccata agctcgcttt gctttccctt caatcttgcc
     2101 gactgtttgt acaggatgtt cctgtcctta taaccctgct cgatcttggt ttgcaggata
     2161 ctgaaagggt taagctcgat ggccagcccg atggagtaat ttgccacaaa ctccgaaagg
     2221 tatttcagcg cgcgccagaa cttccggttg gaaatggtta aaagcaggaa gccgcatacg
     2281 atgccgccga tcaccaggtt gacgaggttc cacgtcacct ttaccagcca gggcaataca
     2341 taggtcataa acccgtaggt gagtccggcc agcagggcca ggcccacgac ggtcgtagcg
     2401 acatttttcg gttcgctgaa ccacccgggt gtttttgttt tttgaatcag cgtgttcatg
     2461 gtcgtttgag tcttaattat gataaataat ggttgatctt ttcgatatct ttctctatct
     2521 gctgccgccg gcgttcgcat tcggcagtgt aagcggtgcg gctgccctcc agtttttcct
     2581 ggttctcccg gatatccttc tgcatcgtgc cgatctgttt ttgcagttcg agtatctgtt
     2641 gtgagagctc ctggatccgg cggttctttt cttcggcttc ggcggacctg ttctgcactt
     2701 tttcctgcag ggccgagtcc accgtttttt gaaaggaccc ggcatcctcg ccgaggacac
     2761 gcaggtattc gccggcagtc gagaggagct tgtctttgtc gagaccttgc gcctgcaatc
     2821 cggcgaacgc ggctacatat ctcgaccgct cgtcgggtat cacctgcatg gcgacgatca
     2881 tccgggcgaa ttcataataa tccggtccgg ggatattcgc atcggtgaac agcttgtcga
     2941 aatgggcggt aaaccgctga tcgcccggtc cgtcgcctgc actcccggca agcggaggtt
     3001 ggttcggtaa ggaagcggcc gccgcggctg tattcgcggt ctcctccgcc ctgtaattcg
     3061 gttgttcgtc accgttatca acgaagaccg acaggatcct tttgccaatg ttggccatag
     3121 ctatagtttt agatggttaa aaaatgcgga ttattggagg agcaggcggg acagcatcat
     3181 cccctcccgc cagccggacc gaacgcaatt ctttgcgcgg atacaagcct ttgctggcct
     3241 gtctttagca gggcgggccg tcagttgacg gtaaataatg gatatgagaa gggtgaacag
     3301 gatggctgcc accccgatga gctttttcat gtttttttca agcttggctt gactctcgtt
     3361 atgttattag tgggaagttt agcggaaaaa aaggttttcc gtcagaacgc atcagctatg
     3421 tggaagaaat ttcattttac aggggaggct ggctccgttt gttttgatga agtaaagtta
     3481 atacgggttt gtgcttccct gttagtatat ccggtaggag cggctcgttt tatgtaatga
     3541 cggctcccct cctgtgataa gcggcaaaat aggtggttga atgacaaata tcggtgatcg
     3601 gcggcggttt ttgactgacg aacggcttcc ggaccccacc agcggcaaga tttgaaaacc
     3661 tatgggtgtt agcttgttac gccgttgcat tttatttcac ccgagcattg ggcaaatcgt
     3721 cgcatccgga ttcgagagcc gtcgtaacga acccgaacaa gcgcaggact attgggtgaa
     3781 gaggacgcga ccccgcccgt tttctcaaca gcatcggcgt agcggctttc atcgttaagt
     3841 atcggttgtt tcgggaagac gccgcgaagg tgccggtagg gaagaagtag ggacaaaaaa
     3901 atccccggta ggaataccgg ggatgtacta atcaaagttc ttttaaccat taaaccttat
     3961 cctatgaaaa gccaaaatta cgtcggccgg acggcatttg ccataaatga tttcaggttg
     4021 ttcatacttt aggaactaac ctcaaaaaaa ccttgggaaa tcacttaaaa atccctggtt
     4081 tttcattgcc catgcaacgc cgctgcggtc aatcgggcta cccgctaagg gtattttgga
     4141 cgaatactgc atttttcctg cggtatgcgc tcgagagaaa atcctaaaat tctggttacg
     4201 aggcaacacg aaatcctgag aacccgttct ttttagtatc ttcaccggct aactataatt
     4261 tctaacaggc gctatgtcaa aaagaaccga ctttgtttat tccgttgacg tggaacccca
     4321 tcggatccgt accaggcaga tcctgaaaca gcatccggaa atgagaaaac tcatcgggaa
     4381 gaacaaatac accatcttcg ccgttttggg cctggtcctt tttcaggtgc tccttgcctg
     4441 gctcgtaaag gatcgctcat ggtggatcat tgtcggtgca gcctatctgc tgggcgcctt
     4501 tgcggatcat tccctgttcg tgatgatcca cgagtgcgcg caccatttat tgttcaagaa
     4561 ccgggcggca aaccggcttg ccggcatcct cgccaacctc ccgcagctgt tccccagctc
     4621 ggtatcattc gagcgctatc atatcaaaca tcattctttt cagggaatac atgagctcga
     4681 tgcggacctg cccaatcgct gggaggcgcg gctgatcaat aactttttta tcggaaaggt
     4741 catctggctg ttgttctatc cgcttttcca ggtattccgc atctcccgca tacgcgaggt
     4801 caagccattc gacaaatggg tggcgctcaa ctgggtggcg gaagctgcct tcatcaccgg
     4861 catctggatc gcttttggtc cgaaagccat cgcatacctg ctgctcagct tctttttttc
     4921 ggttgggctt caccccctgg gcgcccgttg ggtgcaggag cattacctca ctcatgacga
     4981 tcaacaggag accttcagct actacggcgt attgaatacc gtctctttca acgtcggata
     5041 tcacaacgaa caccacgatt tcccttcgat cccctggaac cggctgcccc ttatcaagaa
     5101 gacagcgcct ggtttctacg ataatctgca atcgcataag tcatggaccc tgttgttctt
     5161 ccgcttcctg ttcgataagg aaatatcgtt gttctcccgc gttgtccgga aggacagggg
     5221 caaggtggcg ctcacggacc ggtcgatccc ggaccgcgag ctcgtcgagc aggcgtaacg
     5281 ctaaatgaat acccgcgttc ccgaattgaa actctcccgg aactctttcg gcgtacaagt
     5341 ctttttcttc ttgaaaatgc ggttgaagtt ggagatattg ttgaacccgc agtgatagga
     5401 gatctccgaa atggaatgcg tcgtatcgat cagcatccgc gacgcatgcc ccagccttat
     5461 ttcattcagg ctatctatga acgtattgcc cgttcttttt ttgataaacc ggctgaacga
     5521 cacttccgtc atactcacca gcctggccac ctctcccagc gttatcgact tatcatagtt
     5581 gttgttcatg tattcgaatg ccttttcgat acggcggctg ttgtaagtga aatgctgatt
     5641 cgtgaacgag gcatccgaca aggtccgcat attccgggaa gtagagagat catgaagtat
     5701 cgacaaaagc tccaggacag aatcgaaacc atttttctga ttgagcgaaa ggatacgggg
     5761 cacaagaccg gcggtcgtct cacgcgagaa aaggatccct ttctgtgacc ggtccatcat
     5821 gttgcggata aaagccagct gattacgccg aagcagcttt tcttcgaaaa gatctttatg
     5881 ccattggatc gtcacctcct tgatctcctc gcttttacac tgatgtgtaa accaggcatg
     5941 ataaaggttg gggcccacca gcaccagctc gacatcgtcg atggtatcga tatggtcccc
     6001 gacgatccgt ctggcaccct ttgcattcag gatcagattc aattccagct cgtcatgata
     6061 atgaagggga aaggtaaact cctttttgac ccgcgaaaaa atggtgaaac aatcgttttg
     6121 ggtcaatggc gtgatctcac gaatgatatt cgtactcata ttgcaatagc gttagttaag
     6181 gcgaaggtta aggtaagaca aaaacgggat aaaaaagtat attcagatgg tctattcaag
     6241 ttaatttgat aagtgattat ctatcattct tgtatgacgg agcgatgatc aaacgtgaac
     6301 gaaaaatgaa attttgggat aaaatagtag tagaatagta aacggttgga cccctacctt
     6361 cgaaacccgc gccggcggcc cgtcacgccg cccaatgggg ggaaattgct attttacctg
     6421 aatgaaatta cagctaccgg atcttgccat tatcattgcc tacctggtga ctatggtggt
     6481 cattggaata aggttgcgta aaaaggcctc aaagaacaag gtgaattacc tgttgggtgg
     6541 aaagtcactt ccctggtata tgctggggct cagcgatgct tcggatatgt tcgatatttc
     6601 gggcacgatg tggatggtga gcctttgttt cgtatatggc atgaagagta tctggatacc
     6661 gtggctgtgg ccggtgttca accaggtgtt cctgatggtc tatctcgccc gttggctacg
     6721 acggtccaac gctaccaccg gcgcggaatg gatggctacg cgtttcggac aaaccggccc
     6781 ctgggtccgt tcttcccaca atatcgttgt tgccttcgcc ttgctgagct gtctgggatt
     6841 cctcgcgtat ggcttcgtag gtctgggcaa gttcatgcag atcttcattc cctgggaggc
     6901 agtccggcag tatattccgt tccatgtttc tcccgcctat gtgcccgatc tgtacggcat
     6961 cgtttttacc ttgttcgccg tcttttatgc cattcttggc gggatgcata gcatcgttct
     7021 gggtgatgtc atcaaatatg cgatcatgac cgtcgcatgt atcagcgttg ccattatcgc
     7081 catgcttcgt cttcacgaac acgcaaatgg ccttccgatt cccaaaggct ggctcaatcc
     7141 ctttttcgga tggcggctcg atctccactg gaagggcatc gtggaggatg tcggggggaa
     7201 gataagcagc gatgggtttt cccctttcgg ccttttcttc atgatgatgc tgttcaaagg
     7261 gatcgcggcc agtctggccg gtccggcgcc gaactatgac atgcaaaaag tactgagtac
     7321 ccgcagtccc ggggaagcca gcaagatgag cgggttcgta tccattattc tcctccccat
     7381 acggtattcc atgatcatcg gcctcactgt gctggggctt atctatttca acaacatcag
     7441 cggcgaactc gcgacgccgg gaggtaccga tttcgaaaag atattgccgg ctaccatcaa
     7501 cgcattcctg ccggtgggtc tcatgggact ggtgctcacc ggtttgctgg gcgccttcat
     7561 gggcactttt gcaggcacgc tcaatgcggc acaggcctac tttgtcaacg atgtttatct
     7621 gaaatatatc aacccgtcgg cttccaaccg ccaaaccatt tcgatgaact acctggtggg
     7681 tatcgtcgtc gtcgtcatag ggatcgggtt ggggctattc gtagaagatg tcaatacgat
     7741 cctgcagtgg atcgtatccg gcctctgggg aggatacgcc gcggccaatc tcctgaagtg
     7801 gcattggtgg cgattcaatg caggcggcta tttctgggga atgctggggg gcatccttcc
     7861 ggccatcgcc ctcgcgttcc tgaaatataa aggagtactg catggactcg atctgtatta
     7921 ctggccggtg atctttttgc tgtcggtggc cggttcgatc atcgggtcat atagctgccc
     7981 gccaaccgat ccggaggtcc tgaagagctt ttaccgtacc gtgcggccat ggggatgctg
     8041 gggaaccgtt caccgcctgg tgctgaagga agatccagct ttcagcggca accggaattt
     8101 caaacgcgac atgttcaatg tcgcattggg catcatcggc cagctatgtc tgaccctgct
     8161 tcccatgtat atcgtgctgg gacaaaaact gtcactgttg ctgaacgtga tcctgctgct
     8221 ggcgatcgca ctcattctaa aaaagacctg gtggaacagg cttaaagatt attgaataca
     8281 ccatactaaa atgaccacat gatgaatcat cattttaaag agagaatggc gcagctcaat
     8341 aaacaatact ccgaacttat ccggagaccc aatgagccgc tggtaccggg taacggtatt
     8401 ttccttcgtt acaaaagtcc gatactgacc gcggcccata cccccctttt ctggcgctat
     8461 gacctggacg agctctcgaa cccctacctg atggaacggt tcggcatcaa tggggtcttc
     8521 aatgccggtg ccatcaaatg gaaagaccgc tatgccctga tggtccgtgt ggaaggctgg
     8581 gaccggaagt ccttttttgc cattgcggaa agtcccaacg gtatcgacaa tttcaggttt
     8641 cgcgattcac ccatccacat gcccggctcg ggccggccgg aaaccaatat ttatgatgtc
     8701 cgcctgaccc gtcacgaaga cggctggatc tatgggctct tttgcactga aagtaaggac
     8761 cccgatgcgc cggtctccga tcagtcagcg gcagtagccc agtgcggaat tgcccgcacc
     8821 ctcgatcttg tcgactggga aagactggat gacctgcgga cgcgaagtcc acaacaacgt
     8881 aatgtggtgc tccatcccga atttgtagac ggcaaatatg ccttttatac acgaccccag
     8941 gaaagctttc acgcgacggg caccggcggc ggcgtcggtt tcggtctttc agattccatt
     9001 acgaatgccc tcatcgattc ggagacgatc atcgatcccc ggctttatca tacgatcaac
     9061 gaggccaaga acggccttgg acccggaccc atcaggacaa gactgggatg gctgcatctg
     9121 gctcatggcg tgcgtaacac tgccgccggt cttcgttata ccctttatct cttcatgacc
     9181 gatccgaacg atctcacgaa ggtcatctac aagcccggtg gctattttat cgctccccgc
     9241 ggggaggaga gggtagggga cgtctccaat gtcgtcttct gcaatggctg gatagccgat
     9301 gacgatggtc aggtttatat ctattatgct tcctcggata cgcggatgca tgtcgctacc
     9361 accacgatcg aacagctgct cgattatgtc gtcaataccc ccgaagacga aggcagctcc
     9421 gcgtcgtcgg tgtattcgct tcaacagttg atcgataaga acctggcggc acgcgaaatt
     9481 ctgcggattt tcccgtaggt ttgatctaaa atcagaccac aatgcgttcc ctctcccgga
     9541 tagctatact gctgtcgctt ttcatcggca ccggcctgct gagttctttc caccgcaaac
     9601 ccaaaaaagt gatctttttc ggcgattcca ttaccgaaca aggcgccaag ccgggaggtt
     9661 atatcgtgaa aatgaccgac gcgatcgccc agaagaatct tgctaacgat tactcgctca
     9721 tcggcgccgg tgtaggcggc aataaggtct acgatctcta cctgcgcatg gatgacgacg
     9781 tcctgtccca aaatccggac gttgtgatca tctttatcgg cgtcaacgac gtctggcata
     9841 agacactgct cgggacggga acggacatcg ataagttcga aaaattctat atcgcgatca
     9901 tcaacgagct tttggacaga cacatccgga ctatcctttg tactcctgcc gtcatcggcg
     9961 aaaagacgga tttcaccaac cagcaggacg gagatatgaa cgcctattca caggtgatcc
    10021 gcaacctggc ccagaaattc cattgcgacc ttatcgactt gcgcgagata tttcacaact
    10081 atgagctgaa gaataaccct gcgaacaaag catccggcat cctcacggtc gacaaggttc
    10141 acctcaatga cgccggcaac cagctggtgg cggataagat gctggaggtg ttattgtcca
    10201 aatagcgttt cattttcaaa acggccatta tgcaaaagac catactgatc acgggcgcca
    10261 acggcaacct gggcgctgcc accgtccgca gatttctcga taggggttat aaggtcatcg
    10321 ctgtcgacca atccggtaca cacctgggat tcgcggcgga ccatgatgat ttcgagctcc
    10381 ggtctgtaga cgccggcaat gagtccgttg tcggggcgtt cattgaggag ataatcgagc
    10441 tttatggccg gatcgacggg gcattgctcc tggtcggcgg gttcgctatg ggcgacatcg
    10501 cttctaccga cagcgaagcc ctccggaaat tatatgcgct taacttcgag accacctgga
    10561 attttgcccg tccgctgctc aggcacatga tggagaatga ctacgggcgt atcgtcttca
    10621 tgggcgcccg tcccgctttg atacccgacc agggaaaaca tgtgctggcc tatgcgctta
    10681 gcaagtccct gcttttcgag cttgcccgac tactcaacgc gacggcaaaa ggaaagaatg
    10741 tcgtgacctc cgtaatcgct ccttccacca tcgatactcc cgtcaatcgt caaagcatgc
    10801 ccgatgccga cccgtcggcc tgggtcagac ctgaggagat cgccgccctg atggatttta
    10861 tttgcagcga agaagggaag gcgctgcgcg agcccgtcta taagatatac aacaattcct
    10921 gaaaataagc ggcctcc
//
LOCUS       DASYSV010000474        16363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1380735, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000474 DASYSV010000000
VERSION     DASYSV010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 16363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16363
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380735"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VGQ51_14600"
     CDS             <1..843
                     /locus_tag="VGQ51_14600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV9037861.1"
                     /translation="TFGNMSWRSLRNDRQLTTIPPSIGINTGSWLLRQKKRIYRDSDL
                     TIVTPSVWLKELAVQSPVFDHIPVHQIYNGVDTSVYRPLDKKAVKRKLGIPEDQPTII
                     FSSHYLTVDNPWKGGRDLVEILRKLDQLTGRTISFLALGDGRMDELDELANLHIRYTG
                     YIRNEPDMVDCLNAADLLIYPTRADNLPNVLVESIACGTPAVTFDIGGNTEIIEHDYN
                     GVVIPPFDLDAFAHSTLSLLEDPERRMAFSANGLVKVQQRFEERTMVDRYYLLFEQIK
                     ENHR"
     gene            851..1603
                     /locus_tag="VGQ51_14605"
     CDS             851..1603
                     /locus_tag="VGQ51_14605"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEV9037862.1"
                     /translation="MLTKLKTRIKRLFFPDIHYRLHRMPGSLKKLGTDYGGWIIPTDR
                     LNEGSVCYLAGAGEDISFDVLVAGLFHCDVYIFDPTPRAKKHFERVVASASGGDPITL
                     YGKHRYAIDEETARHLHFKEIGIWKKADQVRFFAPKDETHVSHSISNLQETESWFLAT
                     VERLSDIMRRNGHQRIDLLKLDIEGAEFEVIDTILEDKLPIGILCVEFHAGNGDLDPT
                     RAAVKKLEDHGYAVVARENLDFTFVNLGLPNV"
     gene            1619..2851
                     /locus_tag="VGQ51_14610"
     CDS             1619..2851
                     /locus_tag="VGQ51_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037863.1"
                     /translation="MNKRITVDKYLPVVLLYFFFNGLFLPLGLLYTSILAPVFLYWNL
                     NARTMKQLLVFFAVALPFVVVHFINGVEVNYYLRSLVLVVAIYIFLIAFHRFVHECKT
                     LRTQYKMILLVNIVGVAIAFIALAVPAWRHRFWYTNTVTGNIITTRLQMLTYEPSYYS
                     TLFAPIALYYYLKMIVLKLPDVKTTAILVTVPLLLSLSFGVIIGMAVSLGLTLLYGSR
                     AFFTNKYLAVQLLFGSLGFLLLIGLAMWLFPHNVFILRLKNVFAGHDNSFNGRTTDSF
                     YLAWAIAKTKSVWFGCGLGQTKVIGLDLFRHFYNYAGFTVDSVAIPNVVGDTLATLGI
                     IGLLIRFGLQVYFFFATRVWNNYYRLSLFLFIFIYQFTGSFLTNTAEYVIWLLAFSPG
                     IFEEFDRKNFYINKVERP"
     gene            2848..3930
                     /locus_tag="VGQ51_14615"
     CDS             2848..3930
                     /locus_tag="VGQ51_14615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV9037864.1"
                     /translation="MKVLFIARSTLYKDKGGDTVQVLRTADHLRSAGVEVVVKLTTDH
                     IDYEAYDLLHFFNIIRPADILRHIRLSGKPYVVSTIFVDYSEYERKARGGFTGLLSHV
                     VSSDRIEYMKVVARCLVNGEKIVSPSYLFYGQRNSIRKIIRGAAMLLPNSLSEYNRLY
                     AHYRQQQRYTVIPNAIDPRLFRSPAVPPERDGRLVICVGRIEGRKNQLNLIRALKGSL
                     YQLIIIGSPSANQSGYYEECKKTADRNVSFVAATDQETLLDYYLRAKVHVLASWFETT
                     GLSSLEAAAMGCNLVITDKGDTREYFGNDAWYCDPDSLSSIREAIEQAANATSGGPLQ
                     NKIFAEYTWTNTAEKTAGAYRAVLGK"
     gene            complement(3979..4767)
                     /locus_tag="VGQ51_14620"
     CDS             complement(3979..4767)
                     /locus_tag="VGQ51_14620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9037865.1"
                     /translation="MLVTIITIVYNGEKYIEDTIRSVGAQTYPNIEYIIVDGGSTDST
                     PDIIGKYRDTVTTLIMEKDEGISDAFNKGLRLAKGEIIGIINADDWYEPDTVEKVVAA
                     MPDHDIVYGRLRLWKDDRPDFVCEGDHNYLVSEMTINHPTVFARKEVYDRWGGFDKKY
                     KCAMDYDLVLRFWINGCRFRYIPAILANMRWGGLSDNRWMLGCRETLSIKNKYLPHRR
                     WRNRLYFYKHILAISAPRILQQFGLHFVTRFYRTNFSRVKKVYQ"
     gene            4830..5924
                     /locus_tag="VGQ51_14625"
     CDS             4830..5924
                     /locus_tag="VGQ51_14625"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEV9037866.1"
                     /translation="MKIGILGTRGIPNQHGGFEQFAEFVAPALVQRGHEVSVYLSSLH
                     PFEADTWRGVDLIKEKDYEDKIGTAGQFFYDLNCILDARKRNFDVILQLGYTSSSIWR
                     RLFPKEAVIVTNMDGLEWKRSKYSQAVQSFLRRAEKWAVAGSDMLIADSKGIQAYLAE
                     KYQKGSTFIPYGASLFTDPDPGALADYGLQAYTYNMLIARMEPENNIETIIKGHSGSD
                     TDEKLILIGNYRNNFGSYLKNRYEDHRTVFLGPNYDLNLLNNLRHFSHLYFHGHSVGG
                     TNPSLLEAMASQALIVAHDNIFNRSVLEEDAFYFSTPSDITSLLNRHPLKRGYDSFIE
                     NNNARIAKNYSWEYITDLLEKCLSEALKKA"
     gene            5952..6935
                     /locus_tag="VGQ51_14630"
     CDS             5952..6935
                     /locus_tag="VGQ51_14630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV9037867.1"
                     /translation="MKRQLKSTAFRKSVDIILSPLTLVSAVWHKFVSSSTVRQMPVSE
                     KIFMSVGVLPIRDHYYQPLVNPRKHLRKPLDQPRILPGIRWNIDEQLELLKQFRYQDE
                     LLAIPFSKKEAGSLHFFYDNPSLCPADAEFLYSIVRHFKPRRVIEIGCGYSTLLAIQA
                     EKKNGAENSSHSCTHICVEPYEMPWLEQLGIEVLRTKVEALPVQTFAQLEAGDILFID
                     SSHMIRPQGDVLFEFLELLPTLKPGVLIHVHDVFTPRDYLKEWVVDRHIHWNEQYLLE
                     AFLSFNDQFRIIGAVNLLKKEYGKSLEEKCPVTAGKPEDEPGSFWMVRNNK"
     gene            6932..8740
                     /locus_tag="VGQ51_14635"
     CDS             6932..8740
                     /locus_tag="VGQ51_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9037868.1"
                     /translation="MKILLKYLQPYRWLVALALLLAAINQVFSMLDPYFFGKLGFDKL
                     ASHPWDSGYYNQNKVWVSQGPRTQSQFIWAAIAFLGIQVSVAMVSRIAKAFQDYFSNV
                     IVQRFGATIFTSGLRHSMALPFSDFEDQRSGETLSILTKVRLDTERFIISFINVLFTI
                     VVGVIFVSIYSFTLHWSIMPIYVLGMVLLSVLTSVLSRKIKRIQRTITTETNALAGST
                     TESLRNIELVKSLGLTDQEVKRLNQNTFKILGLELRKVKSIRALSFVQGTFVNFLRQC
                     ILFVLLYLVFGHRLTAGEVMTMVFYSFFIFGPLQELGNIILTYREAEASLNNFDVLMK
                     KTPEMPPVHPRPLGPIRSLDFRDVIFRHQTAHHNAIDQISFTAEKGETIAFVGPSGSG
                     KTTLMKLLVGLYRPQKGSILYNGLDETAINFEDLRRQIGFVTQDTQLFAGTIKENLLF
                     VNPGASESELQEALKKAACLPLLARAEKGIDTMIGEGGLKLSGGEKQRLSIARALLRK
                     PALLLFDEATSALDSLTEEDITRTIRAISAEGEQITILIAHRLSTIMHADRIYVLEKG
                     VIAEMGDHDGLLAEKGLYYAMWRQQIGERKYAMVNS"
     gene            8758..9333
                     /locus_tag="VGQ51_14640"
     CDS             8758..9333
                     /locus_tag="VGQ51_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV9037869.1"
                     /translation="MNDQIKEIIRESIATKTAVLNDAVLMGGVSQIVELIVGRFRGGK
                     HLYFCGNGGSAADAQHLAAEFSGRFYINRDALPSEALHCNTSYLTAVANDFSYDLIYS
                     RLIKGIGKPGDILVGMSTSGNSSNIVKAFETAREKGITTIGMTGEGGGQLKELSDYLI
                     DIPSRNTPRIQEAHMLLGHIICQLVEAEYFK"
     gene            9339..10043
                     /locus_tag="VGQ51_14645"
     CDS             9339..10043
                     /locus_tag="VGQ51_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV9037870.1"
                     /translation="MIIKEAIVLAGGLGTRLRSEVPDLPKCMAPVGGQPFIAYLTDHF
                     RRAGVERFVFALGYKSDAFDEFFRRHFPDGGYTVSTEERPLGTGGAIRRACTVAEGRT
                     VLILNGDTFFRVRVGELAAAHMTHRADCTICLRPMKDFDRYGVVELDAGHRVRGFREK
                     RFTHEGLINGGVYALDREKFLAGDLPEVFSFEKDYLERSLETRRIYGLVQDDYFIDIG
                     IPEDYRRVQHEIKSLQ"
     gene            10040..11071
                     /locus_tag="VGQ51_14650"
     CDS             10040..11071
                     /locus_tag="VGQ51_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEV9037871.1"
                     /translation="MIYRSKAPLRIGLAGGGTDVSPYSDLYGGAILNATLSLYAHATI
                     QPLEDKTIILEGIDRREEQRFEGGTDLDSLPIDGKLDLLKGVYNRIRQTYGWSGKGFR
                     LSTFVDAPAGSGLGTSSTLVVAIIGAFTEMLRLPLGEYDMAHLAYDIERNQLKMAGGR
                     QDQYAATFGGVNYMEFYKDDKVIVNPLRVKQQYLFELENNLLLYYTATSRESARIIEV
                     QSRNVTDKKERSIEAMHQLKLQAQLMKEALLMGRIHEIGEILDFGFRQKQQMAEGISN
                     AFIDDIYEQARRAGATGGKISGAGGGGFMTFYCPGTSKYKVMEVLAGLKGHCQPYSFT
                     GYGLTTWTI"
     gene            11122..11535
                     /locus_tag="VGQ51_14655"
     CDS             11122..11535
                     /locus_tag="VGQ51_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEV9037872.1"
                     /translation="MEKPNYIWSMMTMKCPRCRKGHMFVDPNPWKLRHTMKMPEKCPE
                     CGQPFELEVGFWYGTGYVSYGLSLVLSVASFVAWCVLVGISTHDYRFFLWMGLNAVFL
                     VAMQPWIMRLSRVVYLYFFLRYDPDYKISKPQQFE"
     gene            complement(11628..14198)
                     /locus_tag="VGQ51_14660"
     CDS             complement(11628..14198)
                     /locus_tag="VGQ51_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9037873.1"
                     /translation="MNRFLPGIALLLLVLAYRPILASGSIKASASILASGLAAPDSTE
                     PGSGSLSGHISDSHGAPLPGATVYIPDLKLGVIADSAGNYRFNTLPSGRYLVEAHSVG
                     FKTLTKTAAITGPLILDFVLSDTYVEESAVVVTGLSKATQIKRNPVPIVAISHDYLAT
                     NISTNAIDALVKVPGVRAVTTGPNVSKPFIRGLGYNRILTLYDGIRQEGQQWGDEHGI
                     EVDQYAIDHVEVIKGPASLSFGSDALAGVVNLIPTQPFPEGKMIGDVVLDYQTNNKNI
                     GGSAMLGATKNGFEWMARISHKQAVDYQDKIDGRVFGTAYNETDGNASLGLHKKWGYS
                     HLDFVAYNDLQEIPDGGRDSLTRQFTRQIDEDANAPGEIVSSHDLNSYTIEPLHQHVQ
                     HYRIYSANSLSLGEYGRLQVNLGFQRSLRQEFSHPTMPATPGLDLRMNTYSYDVKYFL
                     PELGDWNITAGVNGMYQNNLVTNGTEFIIPSYHQFDIGTFLLAKKSFDKLDIAGGIRF
                     DSRAFNNAALFTRPDPVTGFDRPVPGSDTVGADNPFSDYHKNFTGVSGSIGATYNFSE
                     KFSIKANIARGYRAPNIAEISANGVHPGTNFYQLGNPDFKPEFSLQEDIGLIFSSRYV
                     VATLDLFNNVISNYIYDRKLTLPNGMDDTVQSGPGTGLPYFQYVASKAHLYGGEISID
                     IHPVKALHFENSLSLVYGDNNGSQPKSIGDSAQFLPFIPPAHGISELRYEFSSKTSRM
                     THAFVKVQLEYSAAQNRAYLAYNTETPTAGYTLFNAGIGGTFADKKGKTIVNVYLMGD
                     NLFNVAYYDHLSRLKYFFFSPGDTNPTHGLYNMGRNISVKLDFPLNFTLKQAAKPADS
                     "
     gene            complement(14333..16189)
                     /locus_tag="VGQ51_14665"
     CDS             complement(14333..16189)
                     /locus_tag="VGQ51_14665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HEV9037874.1"
                     /translation="MKSSYFIFTLLIGTTLSAQVDKAVDTAAVQRLYYRAMHLTLADS
                     VKYYADSIDRLSVMSGNADMGIESLRLYGWYYENRGDYTRALGFYYTALDSARSHRYV
                     HRQTELLTDLAAVYTQDMKQPQKARGIYQECVRLNKELGDAHSLISSYSNLGAIYNRL
                     GLYDSALYFLREGLRIGRPLEAQGSESLSEIYNNIGNAYYYQKKYAASIDWFRINYQK
                     DLASPLPSRLADLWIDVLNMADSYSEEGAYDSAGKYGELALHLAIQQDSRSKQSDSYQ
                     VLANLARHRGDYKRAFDYQGKWYSLDTALVNSETYKAIAELEQKYEARKRENDNLILE
                     AEITRQRFHNRIATIMAISLFLIALVTAVLFIIKRRANRQLSMTNDLIVRQNMRLSEL
                     NYEKNSLISIVSHDLSTPFATINIYHQLLQNENDNFSSGQKKALDRIGQATRYGEHLI
                     RHILDVEKAQTNQQKIQLENLDLRGFAESILDQFSPVAAKKDIRLHLNCPDRAVTLLS
                     DPHLLGRMLGNLLSNAIKYTLSGKNVWVTVGEDKNAVSIEVRDEGVGIPTDELPHLFS
                     KYSKISSRPTAGEPSTGLGLAIVKRICEELNGRISCDSVAGEGSIFTVILRK"
     gene            complement(16189..16362)
                     /locus_tag="VGQ51_14670"
     CDS             complement(16189..16362)
                     /locus_tag="VGQ51_14670"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV9037875.1"
                     /translation="MEVLRLICKEYSNAEIADKLFLSVSTVETHRKNLIAKLGVNNTV
                     GLVKFALRNNLID"
BASE COUNT         3799 a         4057 c         4568 g         3939 t
ORIGIN      
        1 acattcggaa atatgtcctg gagatcgctc cggaacgacc ggcaattgac caccatcccg
       61 ccctcgatcg ggatcaatac tggatcatgg ctgctccggc agaaaaagag gatctaccgg
      121 gacagcgacc tgacgatcgt cactccgtcg gtatggctaa aggagcttgc cgtacaaagt
      181 ccggtattcg atcatattcc ggttcaccag atctacaatg gcgtcgatac gagtgtttac
      241 cggccgctcg acaaaaaagc cgtcaagcga aagctcggga taccggagga tcagcccacc
      301 atcattttca gcagtcatta cctcaccgtc gataatccgt ggaaaggcgg aagggacctg
      361 gtcgagatcc tccggaagct ggatcagctg accggcagga ccatttcttt tctcgccctt
      421 ggtgatggcc ggatggacga gctggacgag ctggccaatc tgcatatccg gtatacgggg
      481 tatatccgga acgaaccgga catggtggac tgtctcaatg cggccgatct gcttatctat
      541 cccacccgcg ccgacaattt gccgaatgtc ctggtggaat ccatcgcctg cggaacgccc
      601 gccgtcacct tcgatatcgg cgggaacacc gagatcatcg aacacgacta caacggagtc
      661 gtcatacctc ctttcgatct cgacgcattc gcgcatagta cgttgtcgct gctggaggat
      721 ccggaaagaa ggatggcgtt ttcggctaac gggctggtaa aggtgcagca gcggttcgaa
      781 gagcgcacca tggtcgaccg gtattacctg ctttttgaac aaataaaaga aaatcatcga
      841 taagctcaat atgttaacaa aactgaaaac ccggataaaa agattgtttt tccccgacat
      901 ccattatcgt cttcacagga tgccgggttc gctgaaaaag ctgggcaccg actatggcgg
      961 ttggatcatc ccgaccgaca ggttgaacga aggttcggtc tgttatctgg ccggagcggg
     1021 cgaggatatt tccttcgacg tcctcgtagc cgggcttttt cattgcgatg tgtatatatt
     1081 cgatccgacc ccaagggcaa aaaagcattt cgagcgggta gtagcatctg caagcggcgg
     1141 tgatccgatc accctgtatg gaaaacaccg gtatgctatc gatgaggaga ctgcccgtca
     1201 tctgcatttt aaggagatcg gaatctggaa gaaggcggac caggtaagat tcttcgcgcc
     1261 aaaggatgaa acgcatgtat cgcattccat ctccaatctg caggaaaccg aatcctggtt
     1321 cctggctacc gtcgaaaggt tgtccgacat catgcgccgt aacggtcacc agcggatcga
     1381 tctgctaaaa ctggacatcg agggagcgga gttcgaagtc atcgacacca ttcttgaaga
     1441 caaattgccc atcggcattc tttgtgtgga attccatgcc ggtaatggag acctggatcc
     1501 gacaagggcc gcggtgaaga agctggaaga ccacggttat gcggtggtcg cgcgtgaaaa
     1561 tctcgacttt acattcgtta acttaggcct gccgaacgta tgatatatat ggtgtaacat
     1621 gaacaagcgc attactgtag acaaatatct gcctgtcgta ttattatact tcttcttcaa
     1681 cggtcttttt ttgccgctgg ggttgttgta tacctccata cttgcgccgg tattcctcta
     1741 ttggaatctg aatgcaagga cgatgaaaca gctgctggtc ttctttgcag tcgcgctccc
     1801 gttcgtggtg gtccatttca tcaatggagt ggaggtgaat tattacctgc gttcgctcgt
     1861 gttggtggtt gccatctata tctttttgat cgcctttcac cggttcgtgc acgagtgcaa
     1921 gacgctccgg acacagtata aaatgatcct cctcgtcaat atcgtcggcg tggctatcgc
     1981 ctttatcgcg ctggccgtcc cggcctggag acaccggttc tggtatacca ataccgtgac
     2041 gggcaatatc atcactaccc gtctacagat gttgacgtat gaaccatcct actattccac
     2101 gctctttgcg ccgatagccc tgtattacta tcttaaaatg atcgtcctga aattgcccga
     2161 tgtaaaaaca acagcaatat tggtcaccgt cccgttgctg ctgtccctgt ccttcggcgt
     2221 cattattggg atggccgttt cactggggct tacgctactg tatggatcgc gcgccttttt
     2281 taccaacaag tatctcgcgg ttcagctcct tttcgggtcc ctgggttttt tgttgctgat
     2341 cggtctggcc atgtggttgt ttccgcacaa cgtattcatc cttcgtctta agaatgtttt
     2401 tgccgggcat gataattcat tcaacggaag aacgaccgat tctttttacc tggcgtgggc
     2461 gatcgcgaaa actaaaagcg tgtggttcgg ttgcggattg ggtcagacaa aggtcatagg
     2521 gttggacctt ttcaggcatt tttacaacta tgccggattt acggtggatt cggtggcgat
     2581 acccaatgtg gtaggcgata cactcgcaac gcttggtatt atcgggcttc tgatccgatt
     2641 tggactgcag gtttatttct ttttcgcaac ccgggtgtgg aacaattatt accgcctgag
     2701 cttgttcctc ttcatcttta tttatcagtt taccgggagc tttctcacga acacggcaga
     2761 atatgtcatc tggttgctgg ccttcagccc gggcattttc gaagaattcg atcgtaaaaa
     2821 tttctacatc aataaagttg aaagaccgtg aaagtccttt tcatcgccag atccacgctt
     2881 tataaggaca aaggaggaga tacggtgcag gtgctgcgca ccgcggacca tctgcgaagc
     2941 gccggtgtag aggtcgtcgt gaagctgacg accgaccaca tcgattacga agcatatgac
     3001 ctgctgcact tcttcaatat catccgaccc gccgatatcc tgcgtcatat acggctttcg
     3061 ggaaaaccgt atgtcgtctc aacgatattc gtggattatt ccgaatacga gaggaaggcg
     3121 agaggaggtt tcaccgggct gttatcgcat gtcgtttcat cggaccggat cgaatacatg
     3181 aaagtagtcg cacgttgcct ggtcaacggg gaaaagatcg tcagtccttc ctatctcttc
     3241 tacggacagc ggaactccat ccgaaagatc attcgcggag ccgccatgtt attacccaac
     3301 tcgctcagtg aatacaacag gttgtatgct cattaccggc agcagcagcg gtacacggtc
     3361 atacccaatg ctatagaccc ccggcttttt cgaagtccgg cggtccctcc cgaaagggat
     3421 ggcaggctgg tgatctgtgt cggaaggata gaagggcgga agaatcagct gaatcttatc
     3481 cgggcgttga aaggcagcct ataccaactg ataatcatcg gatcgccctc agccaatcag
     3541 tccggctatt acgaagaatg taaaaaaacg gccgaccgga atgtctcctt cgtcgccgcc
     3601 accgaccagg aaaccttgct tgactattat ctacgggcga aggtgcatgt attggcgagc
     3661 tggttcgaaa cgaccgggct aagttcgctc gaggcagcag cgatgggttg caatctcgtc
     3721 atcaccgata agggagacac ccgggaatat ttcggaaatg acgcctggta ctgtgacccg
     3781 gattcgcttt cttctatccg ggaagccata gagcaggctg caaacgcaac ctccggcgga
     3841 ccgctgcaaa acaaaatttt tgcagaatac acgtggacga ataccgccga aaagaccgcc
     3901 ggcgcgtacc gggcggtcct gggaaaataa gatgcatctg cctggttaaa gggtgacacc
     3961 gggaaaatga ccacccgtct actgatatac tttctttacc ctcgaaaaat tggtgcggta
     4021 gaaccgcgtg acgaagtgaa gaccgaattg ctggaggata cgcggggcgc ttatggccaa
     4081 tatatgcttg taaaaataaa gtctgttccg ccaccgccgg tgcggcaggt atttattctt
     4141 tatagacagg gtttcccggc aaccgagcat ccagcgatta tcgctcagcc caccccaccg
     4201 catattggcc agtatggccg ggatataccg gaaccggcag ccattgatcc agaagcgaag
     4261 caccagatcg tagtccatgg cgcatttgta cttcttatcg aatccgcccc aacggtcata
     4321 aacctctttt cgggcaaaga cggtcgggtg gttgatggtc atttcgctga cgaggtagtt
     4381 atgatcgccc tcgcaaacaa agtcgggtct gtcgtctttc cagagccgca gccggccata
     4441 tacgatgtcg tgatcaggca tggcggcaac gactttttcc acggtatcgg gctcgtacca
     4501 atcgtccgca ttgatgatgc cgatgatctc tccttttgcg agccgcaatc ccttattgaa
     4561 ggcatcgctg atcccttcgt ccttttccat gatgagcgta gtgaccgtat cgcgatactt
     4621 tccaatgata tcgggggtgc tatccgtcga tccgccgtct acaattatat attcgatatt
     4681 gggataggtt tgcgctccta cgctccggat ggtgtcttca atgtattttt cgccattgta
     4741 gacgatggtg ataatggtga ccagcatatt acgccgcaaa tctatgaaaa gattcgttat
     4801 tttgcagact ttgccagggg cagttgaaca tgaaaatagg cattctcgga accaggggga
     4861 tacccaatca gcacggcgga ttcgaacaat tcgctgaatt cgtggcgcct gccctcgtac
     4921 aaagaggtca tgaggtgtcg gtatatcttt cttcgctgca tccattcgaa gccgacacct
     4981 ggcgcggggt cgatctcatc aaggaaaaag attacgaaga caagatcggg acggctggtc
     5041 aattctttta tgatctcaat tgcattctgg atgcgcgaaa aaggaacttc gacgtgatcc
     5101 tccaattggg atatacaagc agtagtatct ggagacgcct gttccccaaa gaagcggtca
     5161 tcgttacgaa catggacggt ctcgaatgga aaagaagcaa atattcacag gccgtacagt
     5221 cgtttttgag aagagcggaa aaatgggccg tagcgggtag cgatatgctc atcgccgact
     5281 cgaagggtat ccaggcttac ctggcggaga agtatcaaaa gggttcgaca ttcattccct
     5341 atggagcttc gctttttacc gaccccgatc cgggcgcgct ggcagactat ggcctgcaag
     5401 cgtatactta caacatgctc atcgcccgga tggagcccga gaataatatc gagacgatta
     5461 tcaaagggca tagcggctcc gacacggacg agaagcttat ccttatcggg aactacagga
     5521 ataatttcgg ctcctattta aagaacaggt atgaggatca ccgtacagtg ttcctgggac
     5581 ccaactatga tctcaacctg ctgaacaacc tgcggcattt ttcgcacctc tatttccacg
     5641 gtcattcggt gggtggcacg aatccttcct tactcgaagc gatggcgtca caggcattga
     5701 tcgtggcgca tgacaatata ttcaaccgca gcgtgctcga ggaggacgcc ttctatttct
     5761 ctactccctc cgacatcact tcgctgttga accgacaccc actgaaacgc gggtacgata
     5821 gttttatcga aaacaataac gccaggatcg caaaaaatta ctcctgggag tacatcaccg
     5881 atctattgga aaaatgtctt tccgaagcgc ttaaaaaggc ctaatttgcg acgaactccc
     5941 tgttatctat tatgaaacgc cagctgaaaa gcactgcatt caggaaatcc gtggatatta
     6001 tcctcagccc tcttacgctg gtcagcgccg tatggcataa attcgtcagt agcagtaccg
     6061 tccggcaaat gcccgtcagc gaaaagatct tcatgtcggt gggcgttctg ccgatccgtg
     6121 accactacta tcagccactg gtaaacccgc gcaaacattt aagaaagccc ctcgatcaac
     6181 cgcggatttt accgggaata cggtggaaca tcgatgaaca gctggagttg ctgaagcaat
     6241 tcaggtatca ggatgagctt ctcgcaattc ccttttcaaa aaaagaagcc ggcagtcttc
     6301 attttttcta tgataatcct tcgttatgcc cggcggatgc ggaattcctg tacagcatcg
     6361 tgcgtcattt caaaccccgg cgggtgatcg agatcggctg cggatattcg acgcttttgg
     6421 cgatacaggc agaaaaaaag aacggcgctg agaatagtag tcactcctgt actcatatct
     6481 gcgtcgagcc ctatgagatg ccgtggctgg aacagctggg tattgaagtt ttgcgaacaa
     6541 aagtggaagc cctgcccgtc cagacctttg cccagctgga ggccggggac atcctgttca
     6601 tcgactcttc gcacatgata cggccgcagg gagatgtgct tttcgaattt ctcgagctcc
     6661 ttccaaccct taagccgggg gtgctgatcc atgtccatga cgttttcact ccaagggatt
     6721 acctgaagga gtgggtcgtg gaccggcata tccattggaa tgagcaatat ctgctggaag
     6781 cctttctttc tttcaatgat caattccgta tcatcggggc agttaacctg ctcaaaaagg
     6841 aatatgggaa atccctggag gagaaatgcc cggtaactgc cggaaaaccc gaagatgagc
     6901 ccggttcgtt ctggatggtg aggaacaata aatgaagatc ctattaaaat atctgcagcc
     6961 ttaccgttgg ttggtagccc tggcgctgct gctcgccgct atcaaccagg tattttccat
     7021 gctggacccc tatttcttcg gaaagctggg attcgacaaa ctggccagcc atccctggga
     7081 tagcggctat tataaccaaa ataaagtgtg ggtaagccag gggccgcgga cccaatcgca
     7141 gttcatctgg gcagcgatcg catttcttgg gatacaggtc agcgtggcca tggtctcccg
     7201 catcgccaag gcattccagg actatttcag caacgtcatc gtgcagcggt tcggcgcgac
     7261 catcttcacc tccggccttc ggcactccat ggctctgccc ttcagcgatt tcgaggacca
     7321 gcgcagcggc gagaccctgt ctatcctgac gaaggtgcgt ctcgatacgg aaaggttcat
     7381 catttccttt atcaatgtgc tttttacgat cgtcgtcggc gtcatcttcg tatccattta
     7441 ttcgttcacg ctgcactggt cgatcatgcc gatatatgta ttgggcatgg tcctgctgtc
     7501 cgtgctgacc agcgtcctca gcaggaagat caagcgtatc cagcgcacca ttacgaccga
     7561 gaccaatgcg ctggccggca gcaccacgga atcgctgcga aatatcgagc tggtgaagag
     7621 tctcggtctg acagatcagg aagtaaaacg ccttaatcaa aacactttca agatattggg
     7681 actcgagctg agaaaagtga agagcatccg cgcgctcagc ttcgtacagg gcaccttcgt
     7741 caatttcctt cgccagtgta tcctgttcgt cctgttgtac ctggtattcg gacaccggct
     7801 taccgcagga gaggtgatga ccatggtctt ctattccttc ttcatcttcg gccccctgca
     7861 ggagctgggt aatatcatcc tcacctatcg ggaggcggag gcttcgttga acaatttcga
     7921 tgtcctgatg aagaagacgc ccgaaatgcc gccggtgcat ccgcgaccat tgggaccgat
     7981 aaggagcctg gatttccggg acgttatatt ccggcaccag accgcgcatc acaatgccat
     8041 agatcagatc agcttcaccg ccgagaaggg cgagaccatc gccttcgtcg gtccctccgg
     8101 cagcggcaag accacgctga tgaaactgct ggtaggattg tatcgcccac agaaaggcag
     8161 catcctctac aacggtctcg atgagactgc catcaatttc gaggatcttc gccgtcagat
     8221 agggttcgtg acccaggaca cacagctgtt tgccgggact attaaggaaa acctgttatt
     8281 cgtgaatccc ggagcatcgg agtccgaatt gcaggaagca ttgaaaaaag cggcctgtct
     8341 gccattgctc gcgcgtgccg agaaagggat cgacaccatg atcggagaag gaggattgaa
     8401 actatcgggt ggtgaaaaac aacggttgtc gatcgcgcgc gccttgttaa gaaagcccgc
     8461 gcttttgctc ttcgatgagg ccacctcggc gctggattcg ctgacagaag aggacatcac
     8521 gcggaccatc cgggcgatct cagccgaagg cgaacagatc accatcctga tcgcgcatcg
     8581 tctttcaacc attatgcatg cggacaggat ctatgtcctt gagaaaggcg ttatcgcgga
     8641 gatgggggat catgacggat tgctggccga aaaggggttg tattatgcca tgtggagaca
     8701 gcagatcggc gaaagaaagt atgccatggt aaattcataa atctgatctt tgcagctatg
     8761 aacgaccaaa tcaaggagat catccgcgaa tcgatagcca cgaaaacggc agtgctgaac
     8821 gatgctgtgc tgatgggcgg tgtgagccag atcgtggaat tgatcgtcgg ccggttccgc
     8881 ggcgggaagc acttatattt ctgcgggaat ggcgggagtg cggcggatgc gcagcacctg
     8941 gcggctgaat tctccggccg tttttatatc aatcgggatg cgctgccatc ggaggcgctg
     9001 cactgtaata cctcctatct tactgcggtg gccaatgatt tcagctacga tcttatctat
     9061 tcacgtctga tcaagggtat cggcaagccg ggggacatcc tcgtcgggat gtcgacctcg
     9121 ggaaattcct ccaatatcgt aaaggccttt gaaaccgcgc gggaaaaagg catcaccacc
     9181 atcgggatga ccggggaagg aggagggcag ctcaaggagc tgagtgatta cctgatcgac
     9241 attccctcgc gcaatacccc ccgcatacag gaggcgcata tgctcctggg acatatcatc
     9301 tgccagctgg tggaagccga atatttcaaa tgacagatgt gatcattaaa gaagccattg
     9361 ttttggccgg tggtttgggc acccggttaa ggagcgaagt gcccgatctg ccgaagtgta
     9421 tggccccggt gggcggccag ccttttattg catacctgac ggatcatttc aggcgggcgg
     9481 gcgtcgaacg attcgtgttt gcgcttggat ataaaagcga tgctttcgac gaattcttca
     9541 ggcggcattt ccccgacggc ggctatacgg tctcgacaga agagcggccg ctgggcaccg
     9601 ggggagccat ccgccgggct tgtactgtag cagaagggcg gacggtgctg atcctgaatg
     9661 gggatacctt tttccgagta cgggtcggcg agctggccgc cgctcatatg actcatcggg
     9721 cggattgtac catctgtctg cggccgatga aggatttcga ccggtacggc gtggtggagc
     9781 tggatgcggg acatcgtgta cggggattcc gggagaaacg gttcacccac gaggggttga
     9841 tcaacggtgg agtatatgcg ttggaccgtg aaaaattcct ggccggggac ctgcccgaag
     9901 tattttcttt cgaaaaggac tacctggagc gatcgttgga aacccggcgt atttatggat
     9961 tggtccagga tgactatttc atcgacatcg gcattcctga agactatcgg cgcgtgcagc
    10021 acgagatcaa atccttacaa tgatatacag aagcaaagcc ccattgcgga taggcctggc
    10081 cggtggaggc acggatgtga gcccctacag tgatctgtat ggcggggcga tcctcaacgc
    10141 aaccctgtcg ctttatgcgc atgcgaccat ccagcccctg gaagataaga ccatcatcct
    10201 cgaagggatc gaccgccggg aagaacagcg gttcgaaggc ggcaccgatc tcgatagcct
    10261 gcccatcgac ggtaagctcg atctgctgaa gggcgtctat aaccggatcc ggcagaccta
    10321 tggctggtcg ggtaagggat tccgtctgtc cactttcgtc gacgctcccg caggatcggg
    10381 gttggggaca tcgtccacgc tggtagtcgc aattatcggc gcgtttaccg agatgcttcg
    10441 gctgccactc ggcgaatacg atatggcgca tcttgcgtac gatatcgagc gcaaccagct
    10501 gaagatggcc ggagggcggc aggaccagta tgcggctact ttcgggggag tcaattatat
    10561 ggaattctat aaggacgaca aggtgatcgt caatccattg cgggtgaaac agcaatatct
    10621 gttcgagctg gagaacaatt tgttgcttta ctatacggcg acaagccgcg aatcggcacg
    10681 aatcatcgag gtccaaagcc gtaatgtgac ggataaaaaa gagcgatcga tcgaggccat
    10741 gcatcagctg aaactgcagg ctcagctgat gaaagaagcc ctgctgatgg ggcgtatcca
    10801 tgagatcggg gagatacttg acttcggatt ccggcagaaa caacagatgg ccgaaggcat
    10861 ctccaacgct tttatcgacg atatctatga gcaggccaga cgtgccgggg caaccggcgg
    10921 gaagatatcc ggggcagggg gtggaggatt catgactttc tattgcccag gaacgagtaa
    10981 atacaaggtc atggaggtgc tggcgggtct gaagggacat tgccagcctt atagttttac
    11041 ggggtacgga cttacgacgt ggacaatttg aaatgaaggg acgacgttag gcccatcggc
    11101 cgaaatagct aactttgcgc aatggaaaag cccaactata tctggagtat gatgactatg
    11161 aaatgtcccc ggtgccgcaa gggtcacatg ttcgtggatc ccaatccctg gaagctccgg
    11221 catacgatga aaatgcccga aaagtgtcct gagtgcggtc agcctttcga gctggaggtc
    11281 ggtttctggt atgggaccgg atatgtcagc tatggccttt cgttagtgtt gtcggtggcg
    11341 agctttgtcg cctggtgtgt tctggtgggc atatcgacgc atgattaccg gtttttttta
    11401 tggatggggc tgaatgcggt gttcctggtc gcgatgcagc catggatcat gcggctcagc
    11461 cgggtggtct atctgtattt cttcttgaga tatgaccccg actataagat cagcaagccg
    11521 caacagttcg aataaaaaag aggccgaccg gataatacct ggaaggcctc tttgctatat
    11581 atggtggggt tagtaccgga gtaataacgt acgaaaaaga ccatttatta tgaatcggcg
    11641 ggttttgcgg cttgtttgag ggtgaaattc aatggaaaat cgagtttgac actgatattc
    11701 cgtcccatat tatagaggcc gtgggtcggg ttggtatcgc ccggtgagaa aaagaagtat
    11761 ttcagccggc tgagatgatc ataataggca acattgaaga gattgtcgcc catgagataa
    11821 acgttcacga tggtctttcc cttcttatcg gcgaacgtcc cgccgatgcc ggcattgaaa
    11881 agggtatatc ctgccgtggg tgtttccgta ttataggcta aataagcgcg gttctgcgct
    11941 gcggagtatt ccagctgcac ctttacgaag gcgtgtgtca tacgcgacgt tttggagctg
    12001 aattcataac gcaactcgga aatgccatgc gcaggaggga tgaaggggag aaattgagcg
    12061 ctatccccga tggattttgg ctgcgaccca ttattatcgc catagacgag cgacaggctg
    12121 ttctcgaaat gcagcgcctt tacaggatga atatcgatgc tgatctcgcc gccatataaa
    12181 tgggctttgg atgcgacgta ctggaaatac ggcaagccgg ttccggggcc gctctgaacg
    12241 gtgtcgtcca tgccgttggg cagggttagc ttgcggtcat agatataatt cgagatgaca
    12301 ttgttgaaga gatcgagggt cgccacgaca taacgcgacg aaaagatcag ccctatgtct
    12361 tcctggaggc tgaactcggg tttgaaatcc gggttgccca gctggtagaa gttggtgcct
    12421 gggtggacgc cgttggcgga gatctcggca atgtttggag cgcggtaacc gcgggcaata
    12481 ttggctttga tggaaaattt ttccgagaaa ttataggtcg caccgatgct gccggagacg
    12541 ccggtgaaat tcttgtggta atcggagaac gggttgtccg caccgacggt atcggatccg
    12601 gggacgggtc tgtcgaagcc ggtgaccgga tcgggtctgg taaagagggc agcgttgtta
    12661 aaggcgcgcg aatcgaaccg gatgccgccg gcgatatcca gcttgtcgaa ggatttttta
    12721 gccagcagga aggtgccgat atcgaactgg tgataagaag ggatgatgaa ttcggttccg
    12781 ttggtgacga gattgttctg atacataccg ttgacaccgg cggtgatgtt ccagtcgccg
    12841 agttcgggaa gaaaatattt cacgtcatag ctatacgtat tcatccggag atcgagcccc
    12901 ggcgtcgcgg gcatcgtagg atggctgaat tcctggcgga ggctgcgctg gaaaccgagg
    12961 ttcacctgca gacggccata ttcccccaga gagaggctgt tggcggaata gatgcggtaa
    13021 tgctggacgt gctggtgcaa gggttcgatc gtatagctat tgaggtcatg ggaggagacg
    13081 atctctcccg gggcattggc gtcctcatcg atctgccgcg tgaattgcct ggtgaggctg
    13141 tcgcgacccc cgtcgggtat ttcctgaaga tcgttgtagg cgacgaaatc gagatgcgaa
    13201 tagccccatt tcttgtgcag ccccagcgag gcgttgccgt cggtttcgtt ataggccgtt
    13261 ccgaatacgc ggccgtctat cttatcctgg taatcgacgg cctgtttgtg tgagatgcgc
    13321 gccatccatt cgaagccgtt cttagtggca ccgagcattg cggagccgcc gatattcttg
    13381 ttattcgttt ggtaatcgag aacgacatcg ccgatcatct tgccttcggg aaatggttgt
    13441 gtcgggatga gattgactac accggccaat gcgtccgagc cgaagctcag gctagccggt
    13501 cccttgatca cctccacatg gtcaatcgca tattggtcca cttctatgcc gtgttcatcg
    13561 ccccattgct ggccttcctg gcggatgccg tcatagaggg tgaggatccg gttatatccc
    13621 agtccccgga taaacggctt ggagacgttg ggccccgtcg ttaccgcgcg gacaccgggg
    13681 actttgacca gggcgtcgat ggcgttggtg ctgatattgg tcgcgagata gtcgtgcgag
    13741 atggccacga tcgggacggg attgcgtttg atctgggtag ctttcgatag acccgtcacc
    13801 accaccgcgc tttcttcgac ataggtgtcc gaaagcacga agtccaatat gagggggccg
    13861 gtaatggctg ctgttttcgt cagtgtcttg aaaccgactg aatgggcttc caccagatat
    13921 cggccggaag gaagggtatt gaaccggtag ttgcccgccg agtcggcgat aacgcccagt
    13981 ttgagatcgg ggatgtagac ggtggctccg ggaagggggg cgccgtggct atcgctgatg
    14041 tgtccggaaa gggacccgga gccgggttcg gtggaatccg gggccgcaag gccggaggcc
    14101 aaaattgagg cagaggcctt tattgagccg gaggccagaa ttgggcggta ggccagcacc
    14161 agcagcagaa gggcaatgcc aggtaaaaat cgattcatgg tccttgtctg ttgaataaag
    14221 atacagcgtc tgcgggtcgg gcctttttgt aacaaggttt caaatttatg ccagtctctt
    14281 tattttcaat gcaaaaaact tgctaagcgg tagttattgt cgctgacctc ctctattttc
    14341 ggagaatgac ggtgaatatg gagccctctc cggcaacgct gtcgcaggat atccggccgt
    14401 tgagctcttc gcagatgcgt ttgacgatgg ccagacccag acccgtggaa ggctcgccag
    14461 ccgtaggtct tgaagatatc ttgctgtatt tggagaagag atggggcaac tcgtcggttg
    14521 ggatcccgac cccttcatcg cgcacttcga tacttacggc atttttatct tcgccgacgg
    14581 taacccacac attcttgccg gaaagcgtgt atttgatcgc attggacagc agattcccga
    14641 gcatacggcc gagcagatgg gggtcgctaa ggagcgtaac tgccctgtcg gggcaattca
    14701 ggtgtagccg gatgtccttt tttgccgcca cggggctgaa ctgatcgagg atagactcgg
    14761 cgaaaccgcg gagatcgagg ttttcgagct gtatcttttg ctggttggtc tgggcctttt
    14821 caacgtccag gatatgacgg atgagatgtt cgccataccg ggtggcctgt ccgatgcggt
    14881 cgagcgcttt tttctggccg gagctgaaat tatcgttctc attctgaagc agctgatgat
    14941 aaatatttat cgtagcgaat ggcgtactca ggtcatgcga gacgatgctt atcagtgaat
    15001 tcttctcata gttgagttcc gagagccgca tattctgccg gacgatgaga tcgttcgtca
    15061 tactaagctg ccggttggcc ctgcgtttga tgatgaaaag aacggccgtt acgagggcga
    15121 tcaggaaaag gctgatggcc atgatcgtcg cgatccggtt atggaatcgc tgccgggtga
    15181 tctccgcttc gagtatcagg ttatcgttct ccctcttccg ggcctcatat ttctgttcca
    15241 gctcggctat cgccttataa gtctcgctat tgacgagagc ggtgtcgagc gaataccatt
    15301 tgccctggta atcgaaggcc cttttgtagt cgcccctgtg acgggcgagg ttggcaagga
    15361 cctggtagct gtcggactgt ttgcttcggg aatcctgctg gatggccagg tgtagtgcca
    15421 gctcgccgta tttgccggcg gaatcatagg cgccttcttc ggaataggaa tcggccatat
    15481 tcagaacatc gatccagaga tcggccagac gcgaagggag cgggctggcc aggtctttct
    15541 gataattgat gcggaaccag tcgatggatg ccgcatattt tttctgatag taataggcat
    15601 tgccgatatt gttgtagatc tccgagaggc tttcactgcc ctgtgcttcg aggggccggc
    15661 ctatgcgaag gccttcgcgc aggaaataca aggcgctgtc atagagcccg agccggttgt
    15721 agatggctcc cagattggaa taggaggaga taagggaatg tgcatcccct agctctttgt
    15781 tgagccgcac gcattcctgg tagatccccc tggctttttg gggctgtttc atatcctgcg
    15841 tatagacggc ggcaaggtcc gtgagaagct cggtctgacg atgaacgtat cgatggctac
    15901 gcgcggagtc gagggccgtg taataaaacc ccagcgcgcg ggtatagtcg cccctgttct
    15961 cataatacca gccataaagc cggagcgatt cgatgcccat atcggcattg ccgctcatga
    16021 cggacaggcg atcgatggaa tctgcgtagt atttgaccga atcggcgagc gtcagatgca
    16081 tggcccgata gtagagacgt tgcacggcgg cggtatccac ggctttatcc acctgggcgg
    16141 aaagggtcgt cccgatcagc agggtaaaga tgaagtatga cgatttcact aatcgatcag
    16201 attgttccgc agcgcgaatt ttacgaggcc tacggtattg ttgactccca actttgcaat
    16261 gagattcttg cggtgtgttt cgacggtgct gacactgagg aaaagcttat cggcgatctc
    16321 cgcattgctg tattccttgc agatcagccg caggacttcc agt
//
LOCUS       DASYSV010000475         8951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1381372, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000475 DASYSV010000000
VERSION     DASYSV010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381372"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..567
                     /gene="nuoK"
                     /locus_tag="VGQ51_14675"
     CDS             238..567
                     /gene="nuoK"
                     /locus_tag="VGQ51_14675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEV9037876.1"
                     /translation="MPINYYIFLSVTLFCIGVAGVLTRRNAIIIFMCIELMLNSVNLL
                     LVAFSKMHHHALAATVPNAGIDGQLFVFFIMVVAAAEVSVGLAIIVMMYRNTHSIDVN
                     FLNRLKH"
     gene            569..2440
                     /gene="nuoL"
                     /locus_tag="VGQ51_14680"
     CDS             569..2440
                     /gene="nuoL"
                     /locus_tag="VGQ51_14680"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEV9037877.1"
                     /translation="MNLLVSLVPLLPLAGFLINGLFRNYLSKSMTGFVGCVTVLAAFV
                     CSVIIFMDVREPGFDALIVTWWDFIAVDNLHIHFAFQIDQLSALFLLIITGVGFLIHL
                     YSTAYMHDEAGPHFARYFAYLNLFVFSMLLLVMGANFIILFIGWEGVGLCSYLLIGYW
                     FKNVGYGYAARKAFIMNRIGDVGFLIAIFLMLNHFGSVDFAEVFGKANSVSVGVMTAI
                     TLLLFLGATGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMIARSHVLYDLA
                     PATQTVVAVIGVATALWAATIALKQNDIKKVLAYSTVSQLGYMFLGLGVGAYTGAVFH
                     VMTHAFFKALLFLGAGSVIHAMGGEQDMRKMGGLGRRLPATHITFLIGCIAIAGIPPF
                     SGFFSKDEILAAAYAKNPIYYWVGVFTALLTAFYMFRLYAMTFRGGFRGTSEQSHHLH
                     ESPAAMTIPLWILAILAAVAGFLGIPEEFAPNAHWLAKYLGPVVGEGSHGESGIWLMV
                     VSVVLALVGVVTAWARYSRKPELGEPTGFGKVLANKWYVDEIYNAVIVKPLDLLAQFL
                     VFIDKNVVDGVVNGIGKLVQYSSRQLRLLQSGLVGGYVLLMVVGILILFLVELFVKR"
     gene            2442..3920
                     /locus_tag="VGQ51_14685"
     CDS             2442..3920
                     /locus_tag="VGQ51_14685"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEV9037878.1"
                     /translation="MIPVLLIAIPLVTGFIGFFFRNEKAARGWALLSSVATLIVAVIA
                     VTVAKNSPVLHADVDWLPDLGSRFSVGIDGLSGILALLTAISFPIIFIATWRDQYRRA
                     WNFYALMLLSQAGLLGVFVATDALLFYFFWELALIPVYFLSSQWGGERRIQATFKFFV
                     YTFVGSLLMLIGIIWIYSHTPDHSFSLKSFYEMGHKISGKDQGWLFWVMFAAFAIKMP
                     IWPLHTWQPDTYEQSPTAVTMVLSAVMVKMGVFGLIRWLAPVVPIGTWAWGDTVSSCC
                     VIGMIYASLIAIRMDDLKRLVAYSSIAHMGLMCLAVFATDTKGMQGVMIQMFSHGINI
                     LGLWIVVDLIERQFGTRKISELGGLAQKAPALAIGLVVVALANVALPLTNAFVGEFLM
                     FSGVFGSTATVYSVVFTVVAMLAVILSAVYTLNMIQRVFYGATNSLTERAVDIRLNER
                     FALGVLVVIIVFVGVYPGPVLNFTKETADFILKSMTIAPGGR"
     gene            4068..5465
                     /locus_tag="VGQ51_14690"
     CDS             4068..5465
                     /locus_tag="VGQ51_14690"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEV9037879.1"
                     /translation="MNAILLSAIWGIVMMFSGIFTQNKTAIRNIAVLGLALLLVVDCL
                     DMTGVWRVNIDTHKMLYFDTFGLLGNAIAFGSTLLLVLLSGRDMEKVGHNLAEYFALI
                     FFVLCGVAVITTFNTLLMLFLGIEIISIPLYILTGAERKNLKGNEASLKYFLMGSFST
                     GLMLLGIAFLYGASATGTFFIDDLWNKTPAVAPVFMAGLLLLLVSMAFKVSAAPFHFW
                     TPDVYDGAPTVFTSFMATIVKVSIFVGLIRLFDEAFGESQAKWQLFVAIIAAATLFIG
                     NVTAVFQQSVKRMLAYSSIAQAGFMLLALLALNGIAKDGIILYAAAYSLATIGIFAIL
                     IRMKDYTFEGFNGLARQQPVLAATCTIFLFSLAGIPLTGGFFAKYYMLASVVATGHFL
                     WLVIFSVICAAVSVYYYFRVIQAMYFKDGDGTTETEPGVGFKGLLVVLAGLVIILGVF
                     PQSLLGQLTKYIYTW"
     gene            5521..6789
                     /locus_tag="VGQ51_14695"
     CDS             5521..6789
                     /locus_tag="VGQ51_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037880.1"
                     /translation="MKFLDIISLAYRTLRGNGLRTGITIAIITFGIMALVGINTAIDA
                     MKQKFTESFSAMGANGFTIHNKKWFNFNGGGVKSHRRGLKEKKSNSNVPITKLQAETF
                     LARYQFPAKVALNFSGVGDAVVSLGDRKTNPNVRVVGGDENYVDLNGFNLASGRNLNE
                     MDVQTGRNVAIIGTTIAERFFGKNPEAPLEKFININNLPFRVVGVLESKGSSFGMNYD
                     NVIITSYTNVRHYFNTNPNASFNIQVKVTDVRLIDGAIDQAEGVFRPVRRLEVTEGDN
                     FNIDKSDSFVALLLKNLSTITWAAIIIGGITLLGAAIGLMNTMLVAVTERTKEIGLMK
                     AVGGRQRNIRRQYLVESVLISLLGAAFGVLLGVLLGNVFSIVLGTSFVLPWLWVIIGV
                     GMCTAVGLLAGLYPAWKAGRLNPIEALRYE"
     gene            7066..7929
                     /locus_tag="VGQ51_14700"
     CDS             7066..7929
                     /locus_tag="VGQ51_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEV9037881.1"
                     /translation="MAETKPTAAPAAKSSSSVQPKKKGNSISWVAPLVCIICGYLIWR
                     FIIGADGNFTKPDPAGGFWPDQQGAKSGLYQMYEGGIIVPILIGALLTVITFCIERFL
                     TVSKATGNGNIAEFIRKVQYHLANKNVDAAIAECDKQKGSVGNVMKAGLRKYKEMITA
                     PDLDVEQKVLAIQKEVEEATALELPMLEKNLVFLSTIASVATLMGLLGTVIGMIRAFS
                     ALGQAGGGEAAQHLSQGIAEALYNTALGIGTSAISIIMYNVFTTKIDGITYGIDESGF
                     TLTQSFGSLYK"
     gene            8067..8741
                     /locus_tag="VGQ51_14705"
     CDS             8067..8741
                     /locus_tag="VGQ51_14705"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEV9037882.1"
                     /translation="MGRAKIPRKSTTVDMTAMCDVAFLLLSFFILATKFKPPEALTVT
                     TPTSVSSKIAPEKDVVMITIDKDGKVYFSVSDGNTQQRQDIIDDIAQRKGISLTTAEK
                     NNFVKNPSSFVGVPFTGLKNLLDINPEEVKNFKQPGIPTQDSTNNELIDWIKSAVTAF
                     EGRHMNILLKGDDAAKYPNFQGVIIALKKNDQMKFQLITNPVLPPPGSELDKEQKTSG
                     SKSSEQ"
BASE COUNT         1891 a         1884 c         2820 g         2356 t
ORIGIN      
        1 gttgatccac aatttgggga tcagcctgtt ccatgattat gtgttgccgt tcgagatcag
       61 ctcggtgctg tttctctcag cgatggtggg ggttgtggtg atcggaaagg gagaggagaa
      121 gaagatgccc ggcgggcagc agtacggcgg ttaaaccgac ggaggaggtt tgcgaagtgc
      181 aacggagcag acattagccc cggtgaggcg ccaacggcgc cgaggaataa atgacatatg
      241 cctataaact actatatttt tctatcggtg accttgttct gtatcggggt ggcgggtgtg
      301 ctgacgcgcc gcaacgcgat catcatcttt atgtgcatag agctgatgct gaactcggtg
      361 aaccttttgc tggtggcctt ttcgaagatg catcatcatg cgctggctgc cacggtgccg
      421 aatgcgggca tcgacgggca gctgttcgtt ttcttcatca tggtagtagc ggcggcggag
      481 gtgagtgtgg gactcgcgat catcgtgatg atgtatcgca acacgcattc catagatgtg
      541 aactttttga atcgactgaa acattaatat gaatctactt gtttcgctgg tccccctgtt
      601 gccccttgcc ggattcctga tcaacgggtt attcagaaac tatttgtcca aatcgatgac
      661 ggggtttgtc gggtgcgtca ccgttctggc ggcgtttgtg tgcagcgtga tcatttttat
      721 ggatgtgcgg gagccggggt tcgacgccct gatcgtgacc tggtgggatt ttatcgccgt
      781 cgacaatctg catatccact tcgcgttcca gatcgatcag ctgtcggccc tgttcctgct
      841 tattataacg ggggtgggtt ttttgatcca tctgtattcc acggcgtata tgcacgatga
      901 ggcgggtccg catttcgcaa ggtatttcgc ctatctgaac ctgttcgttt tctcgatgct
      961 gttgttggtg atgggcgcga actttatcat tctttttatc ggttgggagg gagtcggact
     1021 ttgttcctat ctgctgatcg ggtattggtt taaaaatgtc gggtacgggt atgcggcgcg
     1081 gaaggccttt atcatgaaca ggatcgggga tgtgggattt ttgatcgcga tatttttgat
     1141 gctgaatcat ttcggatcag tggattttgc cgaggtgttc ggaaaggcta attcggtgtc
     1201 cgtcggagtg atgacggcta ttacactgtt gctttttctc ggtgctacgg gtaagtccgc
     1261 ccagatacct ttatatacct ggttacccga cgctatggcg ggtcctacgc cggtgtcagc
     1321 gctgatccat gccgctacga tggtgacggc gggtatctat atgatcgcga ggagtcatgt
     1381 gttgtacgat ctggcgccgg cgacgcagac ggtagttgcc gtgatcgggg tggctacggc
     1441 cttgtgggct gcgacgatcg ctttgaaaca gaacgacatc aagaaagtac ttgcttattc
     1501 gaccgtgtcg cagctgggat atatgttttt gggtcttggg gtcggtgcgt atacgggagc
     1561 cgtttttcat gtgatgacgc atgcgttttt taaagccttg ttgtttttgg gtgcgggctc
     1621 ggtgatccat gcgatgggcg gggagcagga tatgcggaag atgggaggat tgggtcgtcg
     1681 gctgcctgct acacatatta cgttcctgat cggatgtatt gcgattgcgg ggataccgcc
     1741 tttttcgggt ttcttttcga aggatgagat attagcggcg gcgtatgcga agaatcctat
     1801 ttattattgg gtgggagtgt tcacggcgtt gctgacggct ttttatatgt tcagattgta
     1861 tgcgatgacg ttcaggggag gattccgcgg gacgtcggag caatcgcatc acttacatga
     1921 gagccctgcg gcgatgacga tccctttgtg gatactggca atattggctg cggtggcggg
     1981 ctttttgggg atacccgagg agtttgcgcc gaatgcgcat tggctggcga aatatctggg
     2041 gccggtggtg ggtgagggaa gtcatggaga atccgggatc tggctgatgg tggtgtcggt
     2101 ggtgctggca ttggttggag tggtaacggc gtgggcgcgg tacagcagga agccggagct
     2161 gggtgagccg accggtttcg ggaaggtgct ggcgaataaa tggtatgtcg acgagatcta
     2221 taatgccgta atcgtgaagc cgctggatct gctggcgcaa tttctcgttt tcatcgataa
     2281 aaatgtggtg gatggggtcg ttaatggcat tgggaaactg gtgcagtact ccagccggca
     2341 gctgcggttg ttgcagagcg ggttggtggg agggtatgtg ttattgatgg tggttgggat
     2401 attgatactt tttttggttg aactttttgt aaagagatag tatgattcca gtattactca
     2461 tagctattcc gctggtgacc gggttcatcg gatttttttt ccggaacgag aaagcggcgc
     2521 ggggctgggc gttgttgtcc tcggtggcga ccctgatcgt cgccgtcatt gcggtgaccg
     2581 tggcgaagaa cagtccggtg ctgcatgccg atgtcgattg gttgcccgat ctggggagcc
     2641 ggttttccgt gggtatcgat ggattgagcg ggattttggc gttgctgacg gcgatcagct
     2701 ttccgatcat cttcatcgcc acgtggcggg atcagtatcg cagggcctgg aatttctatg
     2761 ccctgatgtt gttgtcgcag gcggggttgc tgggtgtttt tgtggctacg gatgcgctgt
     2821 tgttctattt cttctgggag ctggctctga taccggtgta ttttctttcc tcgcaatggg
     2881 gtggcgagcg gcggatacag gctaccttta aattcttcgt ttatacgttt gtcggcagcc
     2941 tgctgatgct catcgggatc atctggatct attcgcatac gccggaccat agcttttcgc
     3001 taaagtcctt ctatgaaatg ggtcacaaga taagcgggaa agatcagggc tggttgtttt
     3061 gggtaatgtt tgcggcgttt gcgataaaga tgccgatatg gccgttgcat acctggcagc
     3121 cagataccta tgagcagtcg cctacggcgg tgacgatggt gttgagcgcg gtgatggtaa
     3181 aaatgggtgt tttcgggttg atccggtggc tggcgcctgt ggtaccgatc ggtacctggg
     3241 cgtgggggga cacggtcagt tcctgttgtg tgatcgggat gatctatgcg tcgctgatcg
     3301 cgataaggat ggatgacctg aagagattgg tggcttatag ttcgatcgcg catatgggat
     3361 taatgtgtct ggcggtgttt gcgacggaca cgaaggggat gcagggtgtg atgatacaga
     3421 tgttctcgca tggcatcaat atactcggat tgtggatagt cgtggatctg atcgagcgcc
     3481 agttcggcac ccggaagatc agcgagctgg gcgggttggc acagaaggcg ccggcgctgg
     3541 cgatcgggct tgtcgtggtg gcgttggcaa atgtggcgtt gccgctgacg aatgcgtttg
     3601 tgggagagtt cctgatgttc agcggggtgt tcgggtcgac ggcgactgta tatagtgtgg
     3661 tgtttacggt ggtggcgatg ctggcggtca tattgtcggc ggtgtatacg ttgaatatga
     3721 tacagagggt gttttatggg gccactaatt cgctgacgga acgggcggtg gatataagac
     3781 ttaacgagcg cttcgcgctg ggggtgttgg tggtgatcat tgtgtttgtg ggcgtgtatc
     3841 cggggccggt gttgaatttt acgaaggaga cggcggattt cattttaaaa tcgatgacca
     3901 tagcgcccgg agggcgctag cgaagcgaag cggagcagac attggccctg tggaggccgc
     3961 ccgggcggcc gatgcggagc ggagagaaaa atagaaatac agaagttgaa aggagcgtcg
     4021 caaggatgct taatcgaagc actgacgctg ataacataaa actgaatatg aacgcaattt
     4081 tattatcggc catttgggga attgtcatga tgttcagcgg gatctttacg cagaacaaga
     4141 ctgccatccg gaatattgcc gtgttggggc tggcgttgct gctggtcgtc gactgtctcg
     4201 atatgaccgg cgtctggcgt gtgaacatcg acacgcacaa gatgctgtat ttcgatacgt
     4261 tcgggttatt ggggaatgcc attgcgttcg ggagtacgtt gttgctggtg ttgttgtcgg
     4321 ggcgggatat ggaaaaagtg gggcataacc tggccgaata ttttgcattg atctttttcg
     4381 tgttgtgcgg cgtggcggtc attacgacct tcaatacgct gctgatgttg ttcctgggca
     4441 tcgagatcat ctcgataccg ttgtatatcc tgacaggagc ggagcggaag aatctgaagg
     4501 gtaatgaggc ttcgctgaaa tattttctca tggggtcttt ttcgacgggt ctgatgctgt
     4561 tggggatcgc ctttttgtat ggggcgagtg cgaccggcac cttctttata gacgacctgt
     4621 ggaataagac gccggcggtg gcgcccgtct tcatggcggg gttgttattg ctgttggtat
     4681 cgatggcctt caaggtgagt gcggcgccgt tccatttctg gacgccggac gtttatgacg
     4741 gagcgccgac ggtattcacc tcctttatgg cgacgatcgt gaaggtgtcg atattcgtcg
     4801 gattgatccg gctcttcgac gaggcattcg gggagagcca ggcgaaatgg cagctttttg
     4861 ttgcaatcat cgcggcggcg accctgttca tcgggaatgt gacggccgtg ttccagcaaa
     4921 gcgtgaagcg gatgctggct tattcttcca ttgcccaggc agggtttatg ctgctggctc
     4981 tgctcgcact taatggaata gccaaagacg gaatcatatt gtatgccgcg gcctatagcc
     5041 tggcgacgat cgggatcttt gccatcctga tccggatgaa ggattatact tttgagggct
     5101 ttaacggcct cgcccggcag cagccggtgt tggcggccac ctgtaccata tttctgtttt
     5161 cgctcgccgg tatcccgctg accggcggtt tttttgccaa atattatatg ctggcgtctg
     5221 tcgtcgcgac cggccatttt ctgtggctgg tgatcttctc cgtgatttgt gcggcggtca
     5281 gcgtttacta ctatttcagg gtcattcagg ccatgtactt caaagatggg gatgggacga
     5341 ccgagacaga gcccggggtc gggttcaagg ggttgctggt ggtgctggcg gggctggtga
     5401 taatcctggg ggttttcccg cagagcctgc tggggcagtt gacgaagtat atctatactt
     5461 ggtagcagtg cgtgcatctc gccgcaattt tgcttatctt gctgctggac attactcagc
     5521 atgaaatttc tcgacattat ttcgctggct taccgcacgt tgagaggcaa cggtttgcgg
     5581 acggggatca cgatcgccat tatcactttt gggatcatgg cgctggtggg catcaatacc
     5641 gctatcgacg ccatgaagca gaagttcact gagagctttt cggctatggg cgcgaacggg
     5701 tttaccatcc acaataaaaa atggttcaat ttcaacgggg gcggggtgaa gagccatcgc
     5761 agggggttga aagagaagaa gtccaactcc aatgtgccga tcacgaaact gcaggcggag
     5821 acctttttgg cccgttatca gttcccggcg aaggtggccc taaacttttc cggggtcggg
     5881 gatgccgtcg tatcgctggg ggaccggaag accaatccca acgtgagggt agtggggggt
     5941 gacgagaatt atgtggatct gaacgggttc aacctggcct ccgggcgcaa tctcaatgaa
     6001 atggatgttc agacggggcg gaatgtggcg atcatcggga cgacgatcgc agagcgattt
     6061 ttcgggaaga acccggaggc gccgctggag aaattcatca acatcaacaa tctgccgttc
     6121 cgtgtggtcg gagtgctcga atcgaaggga tcttcgttcg gcatgaacta tgacaatgtg
     6181 atcatcacgt cctataccaa tgtccgtcat tacttcaata ccaatcccaa tgcatcgttc
     6241 aacatacagg tgaaggtgac tgatgtgcgg ttgatcgatg gggcgatcga tcaggccgag
     6301 ggtgtcttcc ggccggtgcg ccggttggag gtgacggagg gggacaactt caatatcgac
     6361 aagagcgata gtttcgtggc tttgttactg aaaaatcttt ccactataac ctgggcagcg
     6421 atcatcatcg gcgggatcac ccttctgggg gcggccatcg ggttgatgaa tacgatgctg
     6481 gttgcggtga ccgaacggac gaaggagatc gggttgatga aagcggtcgg tggcaggcag
     6541 cggaatatcc gccggcagta tttggtggag agtgtgctga tcagtctgtt gggggccgcg
     6601 ttcggggtgt tgctgggggt gttattgggg aatgtgtttt ccatagtcct ggggacgagc
     6661 tttgtattgc cgtggttgtg ggttatcatt ggggttggca tgtgtaccgc cgtcggattg
     6721 ctggcggggt tgtatccggc atggaaggcg gggaggttga atcctatcga agcgctaaga
     6781 tatgagtaag ggagggtaag atgtgtgaag cctgttctaa cttgtacgaa tatgaacgga
     6841 acctagtggg atattgggca ttgcaaggat ttggacttta cgaaaattcc ttatcttgtg
     6901 tcactttgtg aaatattata tatccaattt catctttcga aaagcttttt tccttagact
     6961 tgtgcaaatg ttgggagccg cctgaggggg ctcgtccaag gacagatatt agcgatttct
     7021 attacatact tcaatttttt taaaaaacac taaaaacaaa cgatcatggc tgaaacgaaa
     7081 ccgactgcag cgccagcagc taagagctcc tcatctgttc aaccgaagaa aaagggtaat
     7141 tccatctcct gggtagcacc gttggtgtgc attatctgcg ggtacctgat ctggcggttt
     7201 attatcgggg ccgatgggaa ttttactaaa cccgatcctg ccggtggatt ctggccggat
     7261 cagcaggggg ccaagagcgg gttataccag atgtatgaag gtggtatcat cgtgccgatc
     7321 ctgatcggtg cattattgac cgtcattacg ttctgtatcg agcgtttcct gaccgtaagc
     7381 aaggcgaccg ggaatggcaa tatcgccgag ttcatccgca aagtacaata ccatctcgcc
     7441 aacaagaatg tagacgccgc tatcgctgaa tgtgacaagc agaaaggttc cgtagggaat
     7501 gtgatgaagg caggtctgcg caagtataaa gaaatgatca cggcgcccga cctggatgtc
     7561 gagcagaagg tattggctat acagaaagag gtagaagagg caaccgcgct ggaattgccg
     7621 atgctggaaa agaacctcgt gttcctgtct acgatcgcct ccgtcgccac cctgatgggt
     7681 ctgcttggaa cggttatcgg gatgatccgg gccttctccg cactcggtca ggccggtggt
     7741 ggggaagctg ctcagcacct ttcgcaaggt attgccgagg cgttatacaa tacggccctg
     7801 ggtatcggta cttccgctat ttccatcatc atgtataacg tgttcacgac caagatcgac
     7861 ggtatcactt atggtatcga tgagagcggg ttcacgctga cgcagagctt tggttcgctg
     7921 tacaagtgat ttggcggccg gccggagttg agagccgggc ggccgggatt tatggaaaat
     7981 gggaactata gagtctcatt agggtaagaa aacaggatcc gccggcggct atggggccgg
     8041 ggcgggctat caaacaaaaa attacaatgg gaagagcaaa aataccacgc aaaagcacga
     8101 ctgtcgacat gaccgccatg tgcgatgtcg cctttctgct gctgtccttc ttcattctgg
     8161 cgaccaagtt caagccgccc gaggctttga cggtgacgac gccgacttcg gtgtcttcga
     8221 agatcgctcc tgagaaggat gtagtcatga tcacgatcga taaggacggg aaggtttact
     8281 tcagcgtcag cgatgggaat acccagcaaa gacaggatat catcgatgat atcgcccaac
     8341 gaaagggtat ctcccttacg acagccgaga agaataattt cgtgaagaac cccagctcct
     8401 ttgtgggggt acctttcacc ggactcaaga atctgctgga catcaatccg gaagaagtaa
     8461 agaatttcaa acagccgggt attccgacgc aggatagtac gaacaacgaa ttgatcgact
     8521 ggatcaaatc ggctgtgact gccttcgagg gacggcatat gaatatcctg ctgaagggtg
     8581 acgatgcggc caagtatccg aattttcagg gggtgatcat tgcgctgaag aagaatgacc
     8641 agatgaagtt tcagctgatc accaatccgg tgcttccgcc tccggggtcc gagttggata
     8701 aggagcagaa gacgtccgga agcaagagtt cggaacaata gtgtggcgag gatcaggccg
     8761 tgagcctggg cgacacaccg gttttttaag acaattgagg taaacggtta aggttcaata
     8821 gattgattgc gggtcatgct gagaaaataa ggccgataga ccgaaggtca gacaacaaat
     8881 ttgtaaattt ataaaagcat tttactatgg cagagatgga tacttccggt aagggcgggg
     8941 ggcataagaa g
//
LOCUS       DASYSV010000476         5691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2530139, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000476 DASYSV010000000
VERSION     DASYSV010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530139"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VGQ51_14710"
     CDS             <1..611
                     /locus_tag="VGQ51_14710"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEV9037883.1"
                     /translation="GLRALGPAHYGPGRYANGTDSSGRLSDLGKMLLREMERMRIILD
                     ATHLCDDAFWDAMEIFNGAIWASHNNCRALVNHNRQFSDEMIRALIAKGAVIGGALDA
                     WMLVPGWERGVSLPGPMGCGLEKLIDHIDHICQIAGNALHVGIGTDLDGAFGTEQCPY
                     DIDTIADLQQLGPLLEKRGYSVADITNIMHGNWLRFIRDAWK"
     gene            complement(589..1734)
                     /locus_tag="VGQ51_14715"
     CDS             complement(589..1734)
                     /locus_tag="VGQ51_14715"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV9037884.1"
                     /translation="MRLTSTIALILLSFSGFTQQYYLFIGTYTEGSTGVANGSKGIYV
                     YRFDATAGSFQPVSTIATDNPSYLALAPQGNFVYAVNETHEGTTGNVSAFAFDKKTGK
                     LTFLDKQTSGGNDPCYVTVDKHRKWVMVANYSGGSYEALPVRTDGSLAPATQIVQHYG
                     SGPNKARQEKAHVHSTILTPDQKYMVVCDLGMDQLSVLRFNSAATRQPLTPAADSTVH
                     LQPGIGPRHSCFYPGKPFAYIIDELGGTVDAFHYSNGRFSPLQHISSHPAGYKGDIGS
                     ADIHITPDGRFLYASNRGDANSLAIFSIDPATGRLTNKGFQSTMGRTPRNFMIDPTGH
                     WLLVANQNGNNVVLFRIDPRTGMLTDTGKRIEIPSPVCLCVTSTRPG"
     gene            1794..2300
                     /locus_tag="VGQ51_14720"
     CDS             1794..2300
                     /locus_tag="VGQ51_14720"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEV9037885.1"
                     /translation="MRILYFIPVALLAGMVACNNADNKTSTSDTTKAAATDTMAMAKT
                     SGNAVPALPPVPDGAKVYFKNLKDGETVKSPVKVEMGVDGMKLDTAGSMAAGTGHHHL
                     LVDAGDSIPAGTVVPKDSTHLHFGKGQSSTEVKLAPGKHVLTLQCADGLHRSFGSKMA
                     TTISVNVK"
     gene            2397..3107
                     /locus_tag="VGQ51_14725"
     CDS             2397..3107
                     /locus_tag="VGQ51_14725"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEV9037886.1"
                     /translation="MNKTLGKLLFLFILFIGGAGCMKTGTSTMNSASYLSVINEATYT
                     GTTQVYFDNTLVTVQQGISAGTYSTQYGRIKPGTYDVKFVNSAVDTTMGLIPAAVFDT
                     LDFYTMIVYNPPGMGSASQAIKIWDDFSNLSMSNTNYRFFNLCADYPDVDLYLNNNLV
                     QQGRMTADNAVNQTLNGFLAIPSGNYTVTVKKAGTDSVISTMNTGLFQGDAYTIFLGG
                     NVKSHYTPVQVNVLQASY"
     gene            complement(3133..>5691)
                     /gene="gcvP"
                     /locus_tag="VGQ51_14730"
     CDS             complement(3133..>5691)
                     /gene="gcvP"
                     /locus_tag="VGQ51_14730"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEV9037887.1"
                     /translation="FQTMVSDLTGLPIANASLLDEGTAAAEAMTMFFNLKNKDHDHVT
                     IPRFFVDQHVFAQTKDLLITRALPVGIEIVTGDFKKTTLDSSYFGAILQYPDSNGAVH
                     DYRNFTQQAHAAGAHVVMATDLLALTLLTPPGELGADVAVGNSQRFGVPMGFGGPHAA
                     FFTAREEFKRSIPGRIIGISIDANGNRALRMALQTREQHIKREKATSNICTAQALLAN
                     MAAMYAVYHGPEGLTSIAKRVSLLTRTLASELSTLGYANTNEYFFDTVRIKVSDPKAI
                     RHNAEARGLNFHYSDGNISISLDETTSQEDVMDILSVFGHTSGTDLISAIFDLDETLV
                     NIPSGVTRVSEFLTHPVFNSYHSETKMMRYIRSLENKDLSLNTSMISLGSCTMKLNAA
                     TELIPVSWPEFGGLHPFAPADQWKGYGIILAELEDWLSEITGFAATSLQPNSGAQGEY
                     AGLLTIRAYHNANGGSHRDVMLIPISAHGTNPASAVMAGMSVVVVKSDEDGHIDVGDL
                     KEKAVEYSDRLAGLMVTYPSTHGVFEEGIREICSIIHDNGGLVYMDGANMNAQVGLTS
                     PGLIGADVCHLNLHKTFAIPHGGGGPGMGPICVNEKLKPYLPGHVTHAESALRAGSNG
                     QASKDIPEHAVSAAPFGSASILLISYAYIKMLGAEGLTNATRYAILNANYMKTRLEKY
                     YKILYSGANGTCAHEFIVDLRPFKTSAGIEAEDVAKRLMDYGFHAPTLSFPVPGTIMI
                     EPTESEDKGELDRFCDAMISIHAEIRAIEEGRSDKTDNALKNAPHTQQVVVSDNWNHA
                     YSREQAAYPLPYVRENKFWPAISRVNNTYGDRNLICTCEPVSSYAAVDLKKTPA"
BASE COUNT         1249 a         1395 c         1725 g         1322 t
ORIGIN      
        1 atggtttgcg ggcgctgggc cccgctcatt acgggccggg gcgttatgcc aacggtaccg
       61 acagctcggg caggctgagc gatctgggga agatgttgtt acgggaaatg gagcggatgc
      121 gtatcatcct cgacgcaacc catttgtgcg acgatgcttt ttgggatgcg atggaaattt
      181 tcaacggtgc gatatgggca agtcacaaca attgccgcgc gctggtcaac cacaaccggc
      241 agttcagtga tgaaatgatc agggcactta tagccaaggg cgcggtcatc ggcggagcgt
      301 tggatgcgtg gatgctggta cctggctggg agcggggagt atcgctgccg gggcccatgg
      361 gttgtggtct ggagaagctg atcgatcata tcgatcatat ctgtcaaata gccggcaacg
      421 cattgcatgt cggcatcggc acggacctgg atggcgcgtt cggaacggaa cagtgtcctt
      481 atgatatcga cacgatcgcc gatctgcagc aactgggacc cttactcgaa aaaaggggat
      541 acagcgtagc agacatcacc aatatcatgc atggcaattg gctccggttt atccgggacg
      601 cgtggaagta acgcaaagac aaaccgggga ggggatctcg atccgtttgc cggtgtcggt
      661 gagcattccc gtacgcggat cgatacggaa gaggactacg ttattaccgt tttgattcgc
      721 gaccagcagc cagtgtcccg ttggatcgat catgaaattg cgcggtgtcc gtcccatcgt
      781 ggattgaaaa cctttattgg tcagacggcc ggtggcgggg tcgatggaaa agatggcgag
      841 gctgttggcg tcgccacggt tggacgcata gaggaatctt ccgtcggggg tgatatgaat
      901 atcggcgctg ccgatgtcgc ctttatatcc ggcaggatgc gaggaaatat gttgcagcgg
      961 cgagaatcgt ccattggagt aatgaaaggc atctacagtc ccgcccagct cgtcgatgat
     1021 atatgcgaag ggtttaccgg gatagaagca gctgtgacga ggaccgattc ccggttgcaa
     1081 atggacggtt gaatcggcgg cgggtgtcag cggttggcgg gtggcggcgg aattaaagcg
     1141 caatacggag agctggtcca tgcccagatc gcagacgacc atatattttt gatcgggggt
     1201 gagtatggta gaatgcacat gcgctttttc ctggcgggcc ttattggggc cgctgccata
     1261 gtgctggacg atctgtgtcg ctggggcgag tgagccgtcg gtcctgacgg gcagggcttc
     1321 atagctgccg ccgctgtagt tggctaccat cacccatttc cgatgtttat cgacggtgac
     1381 gtaacagggg tcgttgcccc cgctggtttg tttatcgaga aaggtcagct taccggtctt
     1441 tttatcgaag gcgaatgcgc tgacattgcc tgtggttcct tcatgtgtct cgttcaccgc
     1501 atagacgaaa ttcccctgtg gggccagtgc caggtaggag gggttatcgg tcgcgatggt
     1561 gctgacgggt tgaaagctgc cggccgtagc gtcgaaccgg tacacgtaga tgcctttgct
     1621 gccgttggcg acgcccgtcg agccttccgt atacgtgccg atgaagaggt aatattgctg
     1681 ggtaaatcct gagaatgata gtaagatcag agcgatcgtg gaagtcaaac gcataacggt
     1741 ttctgatatt tgcggtaaat ttaagtactt ttatttccta aatcacttct atcatgcgca
     1801 tcctctattt tattcctgtc gccctgctgg caggaatggt tgcctgcaac aatgcagaca
     1861 acaagacctc taccagtgat acgaccaaag ctgccgccac tgatacgatg gccatggcca
     1921 agacctccgg caatgcagtc cctgccttgc cgcctgtgcc cgatggggcg aaggtctatt
     1981 tcaaaaatct caaagacggc gaaaccgtca aatctccggt taaagtggag atgggtgtcg
     2041 acggtatgaa actcgatact gctggttcca tggcggctgg tacgggacat catcacctcc
     2101 tcgtcgatgc cggggacagc attcctgcgg gcaccgtggt tccgaaggat tccacgcatc
     2161 tgcattttgg aaaggggcaa tccagcacgg aagtgaagct ggctccgggt aagcatgtgc
     2221 tgacgctaca gtgtgccgac ggcttgcaca gaagttttgg aagcaagatg gccacgacga
     2281 taagtgtcaa cgtgaaataa tccggatcgg cccgggcgca gcccgggccg atcttcaggc
     2341 aacccgcgca acgaaaaacg cgtagggatt gtcatagaaa cggaaaaaga cacattatga
     2401 acaaaaccct tggaaaatta ctcttcctct tcattctctt catcggaggg gcaggttgca
     2461 tgaagaccgg tacgtcgacg atgaattccg ccagctatct gtccgttatc aatgaagcca
     2521 cctataccgg tacgacgcag gtttatttcg acaatacgct tgtcacggta cagcagggca
     2581 tatcggcggg tacctactca acccaatatg gaaggatcaa accgggtacc tatgacgtca
     2641 aattcgtcaa ttcggcggtg gacactacca tggggctgat cccggcggcg gttttcgata
     2701 cgctcgactt ttatacgatg atcgtttata atccgccggg gatggggagc gcctcgcagg
     2761 cgataaagat atgggacgat ttcagcaatc tttccatgag caataccaac taccggtttt
     2821 tcaatctgtg cgcggattat ccggatgtgg acctttacct gaacaacaat cttgtccagc
     2881 agggccggat gaccgcggat aatgcggtga atcaaacgct gaacggtttt ctggcgatcc
     2941 catccgggaa ttataccgtc accgtaaaaa aggcgggcac cgatagcgtg atctccacga
     3001 tgaatacagg attgttccag ggagacgcct ataccatctt tttgggcggg aatgtaaagt
     3061 cacattacac ccccgtacaa gtcaatgttt tgcaagcttc ttattaaaga tggagcgata
     3121 tgaccccggc ggttatgccg gcgtcttttt cagatcgacg gctgcatagg agctgacggg
     3181 ctcgcatgtg cagatcaggt tgcggtcgcc atacgtattg ttcaccctcg atatagctgg
     3241 ccagaactta ttttcccgga cataaggcag cgggtaggcc gcctgttcgc gggaataggc
     3301 atggttccag ttgtcggaaa cgaccacctg ctgggtatgc ggcgcattct tgagcgcatt
     3361 gtcggtctta tcggatctac cttcttcgat ggcgcggatc tcggcatgaa tggagatcat
     3421 cgcgtcacag aaccggtcca gctcgccctt gtcttcgctt tcggtcggct cgatcatgat
     3481 cgtaccgggg acggggaagc tgagggtagg cgcatgaaaa ccgtagtcca tcaggcgttt
     3541 ggcgacatct tcggcctcga tgccggcgga tgtcttgaag gggcgaaggt ctacgatgaa
     3601 ttcatgggca caggtgccat tggcgccgct ataaagtatc ttgtagtatt tctccagccg
     3661 cgtcttcata tagttggcat tcaggatagc ataacgggtg gcgttggtga ggccttcggc
     3721 gccgagcatt ttgatatacg catagctaat gaggaggata ctcgccgacc cgaagggagc
     3781 ggccgatacg gcatgttcgg ggatatcttt agatgcctgt ccgttggagc cggcgcgtag
     3841 ggcgctttcg gcatgggtga cgtgtcccgg cagatagggt ttcagctttt cattgacaca
     3901 gatgggcccc attcccgggc cgccaccgcc atgcgggatg gcgaaggtct tatgaagatt
     3961 gagatggcag acgtccgccc cgatgagacc agggctggtc aaacctacct gggcattcat
     4021 attggcgccg tccatataga caaggcctcc gttatcgtgg atgatactgc agatctcacg
     4081 gataccttct tcgaatacgc cgtgagtaga agggtaggtg accatgagac ccgcgaggcg
     4141 gtcggagtat tcaacggctt tttctttgag atcgccgaca tcgatatgtc catcttcgtc
     4201 gctctttacg acgacgacgc tcatgcctgc catgacggcg ctggcggggt tggtgccgtg
     4261 tgccgagata gggatgagca tgacatcacg gtgtgagccg ccattggcat tgtgatatgc
     4321 gcggatggtc agcaggccgg catattctcc ctgcgcgccg ctgttgggtt gaagcgaggt
     4381 cgccgcaaaa ccggtgatct cgctgagcca gtcctccagt tcggcgagga tgataccata
     4441 gcctttccat tggtcggcgg gagcaaacgg atgaagacct ccgaactccg gccagctgac
     4501 agggatcagt tccgtcgcgg cgttgagctt catcgtgcat gaccccagcg agatcatgga
     4561 ggtattgagt gaaaggtcct tattttccag acttcggata tagcgcatca ttttcgtttc
     4621 ggaatgatag ctattgaata caggatgcgt caggaactcg gacacccggg taacaccgga
     4681 agggatattg acgagggtct cgtcgagatc gaatatggcg gagatgagat cggtcccgga
     4741 ggtatgaccg aagacagaaa ggatatccat tacgtcttcc tgggacgttg tctcgtccag
     4801 gctgatgctg atgttgccgt cggagtaatg gaagttcaat ccgcgcgctt ccgcgttatg
     4861 acggatagct ttgggatcgc ttaccttgat ccgtaccgta tcgaagaaat attcattggt
     4921 gttggcatag cccagcgtcg agagctcgga agccagcgtg cgtgtcagca ggctcactcg
     4981 tttggcgata cttgtcaggc cttcgggacc gtgatagacc gcgtacatgg cggccatatt
     5041 ggcaagcagc gcctgcgcgg tgcagatgtt ggaggtggct ttttcgcgtt tgatatgctg
     5101 ttcgcgtgtc tggagggcca tgcgaagcgc gcggttgcca tttgcgtcga tgctgatgcc
     5161 gatgatgcga ccggggatcg aacgtttaaa ctcttcgcgt gccgtgaaga acgccgcgtg
     5221 cggaccgccg aaacccattg gaacgccaaa acgctgagag ttcccgacgg ctacgtcagc
     5281 ccctaattct ccgggaggcg tcagcagggt cagggccagc agatcggtag ccatgacgac
     5341 atgtgcgccc gctgcatgtg cctgctgagt gaaattgcga tagtcatgga cggctccatt
     5401 gctgtcgggg tattggagga tggcgccgaa atatgaggag tcgagcgtag tctttttgaa
     5461 atctccagtc acgatctcga tgcctacggg aagtgcgcgg gtgatcaaca gatcttttgt
     5521 ctgtgcgaag acatgctgat cgacgaagaa ccggggaatg gtgacatggt catgatcctt
     5581 attcttcaga ttgaagaaca tggtcatggc ttcggcagcg gccgtgcctt cgtcgaggag
     5641 tgaggcgttc gcgatcggga gacccgtaag atcggaaacc atggtctgga a
//
LOCUS       DASYSV010000477         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_223429, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000477 DASYSV010000000
VERSION     DASYSV010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223429"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..702
                     /locus_tag="VGQ51_14735"
     CDS             97..702
                     /locus_tag="VGQ51_14735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9037888.1"
                     /translation="MTRILIIEDEIIIARFIERQLQTNFDCETSVAVSAAEARAAMET
                     SVPHLLLCDIQLEEDMSGIDLVTELRRRYAFEVIYITSYQHRTVIEKAAATHPAGYII
                     KPIDETQLFAGVQLAITRARNENRTGKAFLRSDTRFTETELQILRLIREKKTTVDIAE
                     TLHLSRYTIKNHRHAICSKLQLHDENNALLKWVVENGHLLK"
     gene            complement(691..1530)
                     /locus_tag="VGQ51_14740"
     CDS             complement(691..1530)
                     /locus_tag="VGQ51_14740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV9037889.1"
                     /translation="MTVYQKFFGCLLTFSLLAACHNNPAPATATSPAGSDSSGIREET
                     IAFPADSVDIHAFVVYNDSVKGRRPAILVLPEWWGMNDYPKMRARMLAKLGYVAMAVD
                     VYGGGRVTENPDTAGVWAATLYRHPERAKARIDAAIDRLKTYDNVDTSKIGAIGYCFG
                     GGVLLNTVRLGDDLKGVVSFHGTLLGTPARKGVLRTKILVCHGNADKFVTPAMVAEFK
                     KQMDSIGADYTFVGYDSATHAFTNPASTATGQKYHMPIAYNGKADTASWEAMKGFFAG
                     LFK"
     gene            complement(1554..>3647)
                     /locus_tag="VGQ51_14745"
     CDS             complement(1554..>3647)
                     /locus_tag="VGQ51_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV9037890.1"
                     /translation="HHGGDAGSWAVYGGSPAVDHYSSLTQIDTANVAGLQKAWIYHCG
                     DVDSNSQLQVNPLIIDGTMYGISPRLKLFALDAASGRLKWTFDPAPKGFEKVWSMNIC
                     RGVAYYRGSETDQRLFYSVGSSLYCIDALTGRPIGSFGDDGRIDLHHDLGRDVSKLYI
                     AGTTPGMIYKDLLIIGDRVAEEADAAPGHLRAYDVHTGKLRWIFHTIPWPGEAGYDSW
                     DDTTAYRHIGGANTWSGFSLDVERGMVFAPVGSASFDFYGGRRLGNDLYADCVVALDA
                     ATGKLIWHFQTVHHDVWDRDAPTPPVLVTVKKDGRTIPAVAQATKSGLIFVLDRLTGQ
                     PIYPVEERRVNDTSELKGERLSPTQPYPTVMPAFARQTLTEADLNHLVPDSSYQDIRK
                     KLAGYHKGWFAPPSREGTLIFPGFDGGAEWGGPSYDPETGIFYINSHEMPCVLTMADA
                     PAAPVSGKVTIGQAGRTLYTGNCMTCHGPERKGGGNYPSLIGVNKKYSRDSLLTLIAS
                     GRRMMPGFKQLSQAQRQAVAAYILGDAVRQKQPFIDSGAKQNDDPYFHLPYTITGYNK
                     FLTREGYPADMPPWGTLTAVDLNQAKIVWRDTLGDYPELKARGIHSGTENYGGSVVTA
                     GGLVFIAATSDSKFRAFNKRTGQLLWETDLPACGFATPSIYSVDGQEYVVIACGGGKL
                     GKKSGDAYVAFALRP"
BASE COUNT          838 a         1156 c          912 g          741 t
ORIGIN      
        1 ccgctcatcc gcgaccttac ccggcaattg aacggtgaga tgacgatcac cagcgacaag
       61 ggactacatt attcctttat gataccggtt tgagacatga caagaatact gatcatcgaa
      121 gacgaaatca tcattgcccg atttatcgag cgccagctgc aaaccaattt tgactgcgaa
      181 acctcggtcg ccgtgtcggc cgcggaggcc cgggcggcta tggaaacctc ggtacctcac
      241 ctgctgctct gcgacatcca gttggaagaa gacatgtccg gcatcgatct ggtcaccgag
      301 ctgcgccgcc gctatgcgtt cgaggtcatc tatatcacct cctaccagca ccggaccgtc
      361 atcgagaaag ccgccgccac gcatcctgcc ggctatatca tcaaacccat cgacgaaaca
      421 cagctattcg caggcgttca gctggcgatc acccgcgccc gtaacgaaaa ccgcaccgga
      481 aaggcatttc ttcggagcga cacccgtttc accgaaaccg aactccagat cctgcgtctc
      541 atccgcgaaa aaaagaccac cgtcgacatc gccgaaacgc tgcacctcag ccgctatacc
      601 atcaaaaacc accggcatgc catctgtagc aagctccagc ttcacgacga gaacaatgcc
      661 ctgctgaaat gggtcgtgga gaacgggcat ctacttaaat aaacccgcga agaaaccttt
      721 catagcctcc cacgaggccg tgtcggcctt gccgttataa gcgataggca tatggtactt
      781 ctgccccgtc gccgtcgatg ccggattcgt aaatgcatgc gtcgcactat cataccctac
      841 aaacgtataa tccgcaccaa tagaatccat ctgctttttg aattccgcca ccatcgccgg
      901 cgtcacgaat ttatccgcat tcccatgaca caccagtatc ttcgtccgca agaccccctt
      961 ccgcgcaggc gtccccaaca acgtaccatg aaagctgact acccctttca gatcatcccc
     1021 caaccgtacg gtattaagca atacaccccc tccgaaacaa tagccgatcg ccccgatctt
     1081 cgacgtatcc acattgtcgt aggtcttcag cctgtcgatg gcagcatcga tacgcgcctt
     1141 ggccctttcc ggatgccggt ataaagtggc ggcccagacg ccggcagtat cgggattttc
     1201 cgtgacccta cccccgccat aaacgtcgac ggccatcgcc acatacccca gcttcgccag
     1261 catccgcgca cgcattttgg gataatcgtt cataccccac cattcgggaa ggacaaggat
     1321 agcagggcgg cgccctttga cagaatcatt gtaaactaca aaagcatgta tgtctacgct
     1381 gtccgcggga aaggcaatgg tctcttcccg gatacccgag ctgtccgacc ctgcgggaga
     1441 agtcgcggtg gccggtgcgg gattattatg acaggccgcc agcaatgaaa atgtcagcag
     1501 acagccgaaa aacttttgat acacggtcat ggtaagacga tttagcgcca aagttaaggc
     1561 ctcaaagcga atgccacata cgcatcgccc gacttcttgc ccagtttgcc accgccgcag
     1621 gcaatgacaa catactcctg tccgtctacc gaataaattg aaggagtggc aaaaccacag
     1681 gcaggcagat cggtctccca gagcaactga cccgtgcgct tgttgaacgc cctgaacttc
     1741 gaatcgctgg tggcggcaat gaacaccagg cctccggccg ttacgaccga tccgccatag
     1801 ttctccgtcc cgctatggat gcccctcgcc tttagttccg gataatcgcc cagggtgtcc
     1861 cgccacacga tcttcgcctg gttgagatcg acggccgtca gcgtccccca gggcggcatg
     1921 tccgccggat aaccttccct ggtgaggaat ttattatatc ccgtgatagt atacggaaga
     1981 tgaaaatagg gatcgtcatt ctgtttcgcc ccgctgtcga taaaaggctg tttctgacga
     2041 acagcatcac cgagaatata agcggcaacc gcctgccgct gcgcttgact cagctgtttg
     2101 aatcccggca tcatcctgcg acccgacgcg atcagcgtca gcaaagagtc ccgggaatat
     2161 tttttattga ccccgatgag cgagggataa tttcctccac cttttctttc gggtccatga
     2221 caggtcatgc aattgccggt atacagtgta cgaccggcct gcccgatcgt caccttaccc
     2281 gagaccggcg ccgcaggcgc atccgccatc gtcaggacac agggcatttc atgcgagttg
     2341 atatagaaga tcccggtctc cggatcatag gaggggccac cccattccgc gccgccgtca
     2401 aatccgggaa agataagcgt tccctcgcga ctcggcggag cgaaccaacc tttgtgatag
     2461 ccggccagct ttttccggat atcctggtaa gaagagtcgg gcacgaggtg gttgagatcg
     2521 gcctccgtca gcgtctgacg cgcaaaggca ggcatcaccg tgggataggg ctgggtcggg
     2581 gaaagccgct cgcccttcag ctcgctggta tcgttgacgc gtcgctcttc cacaggatat
     2641 atcggctgac cggtaagcct gtccagcacg aagatgagcc ccgatttcgt cgcctgcgcc
     2701 acggcaggaa tggtgcggcc gtcttttttc acggtgacga gtacgggtgg cgtcggtgcg
     2761 tcgcgatccc agacatcatg atgcaccgtc tggaaatgcc agatcagctt gcctgtcgcc
     2821 gcatccaatg ccaccacaca atcggcatac agatcgttgc cgagacgccg gccgccatag
     2881 aaatcgaagc tggccgatcc caccggcgca aagaccatcc ctctttccac atcgagactg
     2941 aatcccgacc aggtattggc cccgccgata tgccggtatg ccgtggtatc gtcccagcta
     3001 tcgtaacccg cctctcccgg ccatgggatg gtatggaaga tccaccggag cttgcccgta
     3061 tgtacgtcat aggccctgag atgacccggc gccgcatccg cctcttcggc taccctgtcg
     3121 ccgatgatca gcagatcctt atagatcatg cccggcgtcg tgccggcgat ataaagcttg
     3181 ctgacatccc ggccaagatc atgatgcaga tcgatcctgc cgtcgtcgcc gaacgaaccg
     3241 atgggcctgc ccgttagcgc atcgatgcaa tacagcgatg aaccgacgga atagaacaat
     3301 cgctgatcgg tctcacttcc ccggtaatac gctacccccc ggcaaatatt catcgaccac
     3361 accttttcaa agcccttcgg ggcaggatcg aaggtccatt tcagccggcc gctggccgca
     3421 tccagcgcaa aaagcttcag ccgcggcgag atgccataca tcgttccgtc gatgatcagc
     3481 gggtttacct gcagctgcga attgctatcg acatcgccgc aatgatagat ccaggccttc
     3541 tgcaacccgg cgacattagc cgtgtctatc tgtgtcagcg atgaataatg atcgactgca
     3601 gggctgccgc catacaccgc ccaggacccg gcgtctcccc catggtg
//
LOCUS       DASYSV010000478         5686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1630060, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000478 DASYSV010000000
VERSION     DASYSV010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630060"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /locus_tag="VGQ51_14750"
     CDS             <1..1839
                     /locus_tag="VGQ51_14750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9037891.1"
                     /translation="DLLDCATFLSQKRQLMPYFHEAISLSRKLGFKKGEAICLVWIGG
                     YYKSLQKPDSALSYLDSAILIAGNSNEQWLRRTRAYALFQKALLHETQDNYYSALSDY
                     FAAMKNYDTSDLYKQKMIFIRLASIYEKLFNDEKSLEYYQAALQALQKLVGYGPNIEA
                     AGINTSIAAIYFRRGDIEKTTAYLDRIAPLMPDTMETLVTGAYYRLAGQVALKENKTD
                     SAIFFLTRALQYYDYTRPMHMDVISAVCADLTRSSLATGDLAIAKKYADESVSAARAS
                     GQKDVLADALVAMAEYYNRTGAQSLAYQDLRHATVLNDSVLRAANIRQANNLAALYDN
                     DKKEKAISQLQADERHEQDAVRQNHLLNIIFLIAIATLVAIGFSLYINVSKNRKLERQ
                     QILELEKEKQLMSIEAMLKGQEDERHRVARDLHDSLGSMLAGVKISFSNLRDKMHLAP
                     STAHSYDNTLEQLDRTIAELRKVAHNLMPEALVKFGLNNAVRDFCESIRLAGSTEIIC
                     EQFGPDRDLGNIADVNVYRIVQELINNAINHGKADRILVQLTKTHDKILITVEDDGKG
                     FEVDGLKKAAGIGWTNIQSRVNYFNGLIDIDSAPREGTTINIELIA"
     gene            1890..2522
                     /locus_tag="VGQ51_14755"
     CDS             1890..2522
                     /locus_tag="VGQ51_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9037892.1"
                     /translation="MIRVYIVEDHNVVVEGLRVLLQKEDEIKIVGSAATAADCLAFLS
                     IHPIDVLLMDINLPDMSGVDLCGIVKTRHKSVMVLGLSTFSQGLYMSALMEHGASGYL
                     LKNITRQELIDGIRTANNGGIYFSFEAGRIYKNTLEKKQHQPVLTKREKEIVRLIAEG
                     LTNAQISRVLFISVDTVDTHRKNLYTKLNVNNTALLIRYAIENGLIEHPQ"
     gene            complement(2503..5139)
                     /locus_tag="VGQ51_14760"
     CDS             complement(2503..5139)
                     /locus_tag="VGQ51_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEV9037893.1"
                     /translation="MQKKALLLPLLLLSSATLFAQQSDSYRIPYDDSTLGPQGTTTLM
                     PFNRLVRSAGKVVSFGDSRLENHALDFALLPDGRHLAVEDRYGVGILDLSTQEIVQRW
                     SMRDVPATRSLMSTYSGIKAFGYDGHTYIVWGAGTTSQSALLIAEWDGSAIKNVSAVA
                     MKAVAPAATALPNDIAVRFEGGIPYLYFVLNGNNQLVKMRFTDREIVWTVPTGVAPYG
                     LRIIGNKVYVTNWAGPLVTDSSREYAGTPWGSAYTNPVTGATASGSLSIIDAQSGSLV
                     KELPLGLHPNAIVSSPDSLFLYICNGNSDNVSVVDVRREVVVDSVAVGLFTGGITYYG
                     SSPNALTTDAGGTMLYVANGFDNAVCVVRLGSNAATHGSSGESKVLGYIPTEAYPGGV
                     ALVGNDLYVANIEARGPRVLARFNAAGSVNAYNIHQALASVSIIPVPSKRQLKKYSKA
                     VRQQSLIFRLELTNRAPRHDVAPRPLPERIGEPSVFKHVVYIIKENKTYDQVYGDITQ
                     GRGMPDLCVYGDSVTPNQHRLSGAFSLLDNYYASGKCSAEGHQWADAAMTSDYVEKMV
                     RAWFRSYPHRQEDALVYNRNGFIWNNALDHGKKVRIYGEACTTHYDPSLKWGDIYKGW
                     LDHTDQGFVNTSTIARIRPIISPAYPDCDNLVFTDQLRADIFIHDWKAAEQAGGDSLP
                     DLMVVSLPDDHTAGIAPGMPVPQAMVADNDLAVGRIIDAISHSRFWDSTVVFITEDDS
                     QSGWDHISAYRTTGLVISPYSVIGHAIHTNYNQTGMVRTIEQILGIPPMNVMDATALP
                     LFDCFSDQKTPYVFTHVPNVVPLDRMNKPVAELKGKAATFARASAVQAAKGVDGGNDD
                     LLNRILWFAAKGDVAYPTGDVR"
     gene            complement(5220..>5686)
                     /locus_tag="VGQ51_14765"
     CDS             complement(5220..>5686)
                     /locus_tag="VGQ51_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV9037894.1"
                     /translation="GWVLGCVTSLIFACNEKKEEPAAIVIDKEKIKNEIQAKEDAFAN
                     LYNNGEFKKIGYYADDATSFFQNRPPLVGREAILAFLRSDIMSNADKISFKTNEVFIS
                     NDGNQVVEVGSFKVVDSLNNPFNSGNYISLFEKRNGNYVVLRDMSASEMPLK"
BASE COUNT         1439 a         1738 c         1301 g         1208 t
ORIGIN      
        1 gatctcctcg actgcgccac cttcttaagc cagaaaagac agctgatgcc ctattttcat
       61 gaagccataa gcctgagcag aaagctcggc ttcaaaaaag gcgaagccat ctgcctggtc
      121 tggatcgggg gatattataa aagtcttcaa aaaccggaca gcgccctctc ttatctcgac
      181 tccgccatat tgatcgcagg caattccaac gaacaatggc tccgacgcac ccgcgcatac
      241 gcgctttttc agaaggcatt gctgcacgag acccaggata actactactc cgccctcagt
      301 gactatttcg ccgccatgaa gaactacgac acaagcgatc tctacaaaca aaagatgatc
      361 ttcatccggc tggcctctat ctatgaaaaa ctcttcaacg acgaaaaatc cctcgaatac
      421 tatcaggccg cgctgcaagc ccttcaaaag ctggtgggct acggaccgaa tatcgaagcc
      481 gccggcatca atacctccat tgcagccatt tatttccgtc gcggagacat cgaaaaaaca
      541 acggcctatc tcgaccggat agctcccctc atgccggata cgatggaaac cctcgtcacg
      601 ggcgcatact atcggctggc cggacaggtg gcgctgaaag agaacaagac cgactccgct
      661 atcttcttcc ttacccgggc cctccaatac tatgattaca cccgccccat gcatatggac
      721 gtgatctccg ccgtctgcgc cgatctgacc cggagcagcc tggccaccgg cgacctcgcc
      781 atcgcaaaaa agtatgcgga tgaaagcgtc tccgcagcac gcgccagcgg gcaaaaggac
      841 gtcctcgccg atgccctcgt cgccatggcc gaatactata accgcacggg cgcccaatcc
      901 ctggcctacc aggatctgcg acacgcaacc gtcctcaatg atagcgtcct ccgggccgct
      961 aatatcagac aggccaataa tctggccgcc ctctacgaca acgacaaaaa agaaaaagcc
     1021 atctctcagc tacaggccga cgaacgacat gagcaggatg ccgtccggca aaaccacctg
     1081 ctgaatatca tcttcctcat cgcgatcgct accctcgtgg ccatcggatt cagcctctat
     1141 atcaatgtca gcaagaaccg gaagctcgaa cgccagcaaa tcctggagct cgaaaaagaa
     1201 aagcaattga tgagtatcga ggccatgctg aaaggacagg aagatgaacg ccaccgtgtc
     1261 gcgcgcgatc tccacgacag cctgggtagc atgctcgccg gcgtgaagat ctctttttcc
     1321 aatctccggg acaagatgca cctggctcct tccaccgccc actcctatga caataccctc
     1381 gaacagctgg accgtacgat cgccgagttg cggaaagtag cccacaacct catgcccgag
     1441 gcattggtaa aatttggcct gaacaatgct gtcagagact tctgcgaatc catccggctg
     1501 gcaggcagca cagagatcat ttgcgaacag ttcggccccg accgcgatct gggcaatatc
     1561 gccgatgtca atgtataccg tatcgtccag gagctgatca acaacgccat caaccatgga
     1621 aaggcagacc ggatcctcgt ccagctgacc aagacccacg acaagatcct gatcacagtc
     1681 gaagatgatg ggaaagggtt cgaggtcgac ggtctaaaaa aggcagcggg catcggctgg
     1741 accaatatcc aatcccgggt caactacttc aacggcctca tcgatatcga ttcagcaccg
     1801 cgtgaaggca ccaccatcaa catcgaatta atagcataac atgacacgcc ccaattcccc
     1861 ccaaactccc ccaacattaa tagcataaca tgatacgcgt ctatatcgtc gaagatcaca
     1921 acgtcgtagt agaaggcctc cgcgtccttc tgcaaaaaga ggatgagatc aaaatcgtcg
     1981 gctcggccgc gacagcagcc gactgtctcg catttctctc tatccaccct atcgacgttc
     2041 tcctgatgga tatcaatctc cccgatatga gcggtgtcga tctgtgcggg atcgtcaaga
     2101 ccagacacaa aagcgtcatg gtcctcggcc tcagcacttt tagccagggc ctctatatga
     2161 gcgcgctgat ggagcatggg gccagtggct atctgctcaa gaacattaca cgacaggaac
     2221 tgatcgatgg gatccgaacc gccaacaacg gcggcattta tttcagcttc gaggcgggaa
     2281 ggatctacaa gaatacgctc gaaaagaaac aacaccaacc cgtcctcacc aaacgcgaga
     2341 aagagatcgt ccgtctcata gccgaaggtc tcaccaacgc ccagatcagc cgggtgctct
     2401 ttattagcgt ggacaccgtc gacactcacc gcaagaatct atatacaaag ctcaatgtca
     2461 acaacacggc gttgctcatc cgctatgcga tcgagaacgg cctcatcgaa catccccagt
     2521 aggatatgcc acatcgccct tcgccgcaaa ccacaatatc cggttcaaca gatcgtcatt
     2581 gcctccatct acacccttcg ccgcctgcac ggccgaggca cgcgcaaacg ttgccgcctt
     2641 acccttcaac tccgccaccg gcttattcat ccggtccaac ggcaccacat tcggcacatg
     2701 cgtaaacaca taaggcgtct tctgatccga gaaacaatcg aacaaaggca atgccgttgc
     2761 atccatgaca ttcatcggcg gtatccccaa tatctgctcg atcgtccgca ccatgccggt
     2821 ctgattgtaa ttggtatgga tagcatgccc gatcacggaa taaggactga tcacgagacc
     2881 cgtcgtccgg tatgccgaga tatgatccca tcccgactgc gagtcgtctt ccgtaataaa
     2941 aactaccgtg ctgtcccaga accggctatg cgatatcgcg tcgatgatcc tgcccacggc
     3001 aagatcgtta tcggccacca tggcctgcgg aacgggcatc cccggcgcaa tgcctgcggt
     3061 atgatcgtcc ggcagcgaca caaccatcag atcgggcaaa ctgtcgccgc cggcctgctc
     3121 ggcggccttc cagtcatgga taaaaatatc cgcacgcagc tgatcggtaa aaacgagatt
     3181 gtcgcaatcg ggataggcag gtgagatgat cggcctgatc cgcgcaatgg tactcgtatt
     3241 gacgaacccc tgatccgtat gatcgagcca gcccttataa atatctcccc acttcagcga
     3301 aggatcgtaa tgcgtcgtac aggcctcacc atagatacgc accttcttac cgtggtcgag
     3361 cgcattattc cagataaacc cgttccggtt gtacacaagc gcatcctcct gccggtgagg
     3421 ataactgcgg aaccaggcac gcaccatctt ttcgacatag tcggaggtca tggcggcatc
     3481 cgcccactga tgcccttccg ccgaacattt acccgaggca taatagttat ccagcagcga
     3541 gaacgccccg ctcagccgat gctggttcgg ggtaacgctg tctccataga cacacagatc
     3601 aggcatgccc ctaccctggg tgatatcgcc atacacctgg tcataggtct tattttcttt
     3661 gatgatatat acaacgtgtt tgaagaccga cggctcaccg atcctttccg gtaggggtcg
     3721 cggcgccaca tcatgacgcg gtgcacggtt ggtcagctcc agccggaaga tcagactttg
     3781 ctggcgtacc gccttcgaat atttcttcag ttgccgcttc gatggcaccg gtatgatgct
     3841 caccgacgcc aatgcctgat ggatgttata tgcgttgacg gaacccgctg cattaaaacg
     3901 cgcaagcaca cgcggccccc tcgcttcgat attcgccaca tacaagtcat tgcccaccaa
     3961 tgcaacgcct cctggatatg cttccgtcgg tatataaccc aatactttgc tttcgcccga
     4021 agacccatgc gtggccgcat tgctgcccag ccggacgaca cacaccgcat tgtcgaatcc
     4081 gttggcaaca tacagcatcg ttccgcccgc atcggtcgtc agcgcattgg gactgctgcc
     4141 atagtaagta atgccaccgg taaacaaccc caccgcaaca gaatccacca ctacctcccg
     4201 ccgcacgtcg accacactca cattgtcgct attgccattg catatgtaaa ggaagaggct
     4261 atccgggctg ctgacgatgg cattcggatg cagacccagc ggcaattctt tgaccaggct
     4321 tccgctctgc gcgtcgatga tcgacagact gccggacgcc gtagcgcccg tgacgggatt
     4381 ggtataggca ctgccccagg gcgtgcccgc atactcgcga ctgctgtcgg tgaccaacgg
     4441 tcccgcccaa ttcgtaacat acaccttatt gccgatgatc cttagtccat aaggggcgac
     4501 ccctgtcggc acggtccaaa cgatctcccg gtccgtgaac ctcatcttca ccagctgatt
     4561 attcccattc agcacaaaat acaaatatgg aatgcctccc tcgaaccgga ccgcgatgtc
     4621 attgggcagg gccgtcgccg ccggcgctac ggccttcatc gcaacggcgg aaacattctt
     4681 tatcgcggaa ccgtcccatt cggcaatcag caaagccgac tggctcgtag ttcccgcacc
     4741 ccagacaata taggtatgac cgtcatagcc aaacgccttt atccccgaat aggtgctcat
     4801 cagactcctc gtcgccggga catcgcgcat gctccaccgc tggacgatct cctgtgtcga
     4861 cagatccagg atgccgacgc cataccggtc ctccactgcc aaatgccggc cgtccggcaa
     4921 tagcgcaaaa tccagcgcat ggttctccag ccgcgagtct ccgaagctga cgacctttcc
     4981 cgcagatcgc accaggcgat taaagggcat caacgtagtc gtgccctgag gcccgagcgt
     5041 gctatcatcg taagggatgc gataagaatc ggattgctgg gcaaaaaggg tagccgagga
     5101 caaaaggaga agaggcagaa gaagcgcttt tttttgcatc gttggaggtt ttcggccccg
     5161 aatttaggac agaaagtcca tttccccggc taacgcccct atctattgta atttacattt
     5221 tatttgagcg gcatttcaga agcactcata tctcttaaaa caacataatt tccatttctt
     5281 ttttcaaata gactgatata attgcccgaa ttgaatggat tattgagcga atcgaccacc
     5341 ttaaaagaac ctacttcgac cacctgattt ccatcgtttg atataaatac ttcgtttgtt
     5401 ttaaacgaga tcttatcggc attcgacatt atatcggatc ttaaaaacgc caggattgct
     5461 tccctaccca ccaatggtgg cctgttctga aaaaagctcg tcgcatcatc cgcataatag
     5521 cctatttttt tgaattcgcc attattataa agattggcaa aagcatcttc cttagcctga
     5581 atttcatttt tgatcttttc tttatcgatc actatcgctg caggttcttc tttcttttcg
     5641 ttacaggcga aaatcaagct cgttacgcac cccagcaccc aacctt
//
LOCUS       DASYSV010000479         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1504763, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000479 DASYSV010000000
VERSION     DASYSV010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504763"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VGQ51_14770"
     CDS             <1..588
                     /locus_tag="VGQ51_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV9037895.1"
                     /translation="AIAFVVLLTLKRTHPARKKWAWGGLILSAAYLCYAIGNKMSTDA
                     HAKTDLARDHHHYTRYLSTPTPLNAWLWYVVAEDSGGFYTGYRSVFDHLPVQFRFQPK
                     GGRLLRPFLKARDVRYLLRFSQGYYVVEEWGDTTVFNDLRFGEMKGWEDPKARFVFHY
                     FLQYPEGNRVVVQRGRFAGWDSHTLSIFLRRIRGN"
     gene            complement(585..1823)
                     /locus_tag="VGQ51_14775"
     CDS             complement(585..1823)
                     /locus_tag="VGQ51_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV9037896.1"
                     /translation="MARKKKTSRHLAGYTHHNKVRLVYGGVDFFTTIVQLIDGAKTTI
                     HLQTYIFDADETGRIVSDALLRAASRKVEIFVLLDGYASQHLSKQLIAEWKAAGIHFR
                     WFWPLFKSRNFYLGRRMHHKVIVADGARGMAGGINISDRYNDIGDQKAWLDMALLVEG
                     DAAMKLHLACRDIWTKAYWKTPNLKKDKFAWLEGYTPNEECLVRVRRNDWVQGKNEIS
                     RSYVEMFRHAKSRIIVLSAYFLPGKILRKQMVAAAKRGVVIKIIVGSVSDVKISRLAE
                     QFMYQWLFRNNMQVYEYQDSVLHGKMAAYDGVWMTDGSYNVNRISAYASVELNMDVRN
                     EAFVTKVESELDEIIRHHCVRILPKEYFHRTGLFQRLLQRLAYQTIRLLFFLFTFYFR
                     QEKESKPSERASRQQNIQTR"
     gene            1901..2194
                     /locus_tag="VGQ51_14780"
     CDS             1901..2194
                     /locus_tag="VGQ51_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037897.1"
                     /translation="MKACLRFETWCLGLLLVPCFSSGQNDYHLSGYTYNDAHGSINSN
                     VPLNEINPHAWRHFHNLFPGADGEYWFASGEGYQVSFIQKGYHYQAYFDQRGA"
     gene            complement(2368..2937)
                     /locus_tag="VGQ51_14785"
     CDS             complement(2368..2937)
                     /locus_tag="VGQ51_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037898.1"
                     /translation="MRRILTLALGFTSLTLLYSCGSSNKLSNYTPFTRDLKQKLEVRN
                     IDLKDVQFYVDQKLILNRNLGSEDISVKHGVVKLSNGKSINEVVIPSLTPGLCMGTDN
                     DRLLVSFEKGNSYLAFGLGSGYTANDYVLYGTEWKLGTTLVTYDSEKFRARCGTCSDV
                     ATATLVIKKKELERLERKTRTATGRTVSK"
BASE COUNT          697 a          735 c          813 g          800 t
ORIGIN      
        1 gctatcgcct tcgtagttct gctcactcta aaaaggacac atcctgccag gaaaaaatgg
       61 gcatggggag gccttattct tagcgccgct tatctgtgtt atgccatcgg gaataagatg
      121 agcacggatg ctcatgcgaa gacggacctg gcgcgggacc atcatcacta tacgcgatat
      181 ttgtcgacgc cgaccccgct gaatgcctgg ttgtggtatg tcgtggccga ggatagcgga
      241 gggttttata ccggttaccg ctcggtgttc gatcatctgc cggttcaatt ccggtttcag
      301 ccaaagggag ggcggttgct gcgacctttt cttaaggcaa gggatgtgcg ttatcttttg
      361 agattttcgc agggatatta tgtagtagag gaatgggggg atacgacggt attcaatgat
      421 ctgcggtttg gggagatgaa gggatgggag gatccgaagg cgcggttcgt atttcattat
      481 tttctgcagt atccggaagg gaatcgcgta gttgtacagc gggggcggtt tgcggggtgg
      541 gatagccaca ccctttccat atttttgaga aggataaggg ggaattagcg ggtttggata
      601 ttttgctgcc gggacgcccg ctcacttggt ttggattctt tttcctggcg aaaataaaag
      661 gtgaacagga agaacagcaa acggatcgtc tggtaggcca gcctttgcaa caatcgttgg
      721 aagagacccg tccggtgaaa atattctttt ggaaggatcc gcacgcaatg gtgccggatg
      781 atctcatcga gctctgactc gacttttgtt acgaaagctt cattacggac gtccatattc
      841 agctcgacgc tcgcataagc gctgatgcgg ttgacattat aggagccgtc ggtcatccat
      901 accccgtcat aggcagccat cttaccgtgc aggacagaat cctggtattc atagacctgc
      961 atattgttgc ggaagagcca ttggtacatg aactgctcgg ccagccggga tatctttacg
     1021 tcggaaacac tgcccacgat gatcttgatc acgacccccc ttttcgccgc tgcgaccatc
     1081 tgtttccgga ggatcttgcc cgggaggaaa taagcagaaa ggacgattat ccgggattta
     1141 gcatgccgga acatctccac ataacttcgc gagatctcat tctttccctg tacccagtcg
     1201 ttgcggcgga ccctgaccag acattcttca ttcggtgtat agccttccaa ccatgcgaat
     1261 ttgtcttttt tcagattggg cgttttccag taggcttttg tccatatgtc ccggcaagcg
     1321 agatgcaatt tcatcgcggc atccccttct accagcaagg ccatgtcgag ccaggctttt
     1381 tgatccccga tatcgttgta gcgatcgctg atattgatgc ccccggccat gccgcgggcg
     1441 ccgtccgcca cgatcacctt gtgatgcatg cgtcggccca ggtagaagtt ccggcttttg
     1501 aatagtggcc agaaccaacg gaaatggatg ccggctgcct tccattcggc gatcagttgt
     1561 ttggatagat gctgtgaagc gtagccgtcc agcaacacga atatttccac tttccgcgag
     1621 gcggcgcgca gcagcgcgtc cgatacgatc cggcctgttt cgtccgcgtc gaagatatag
     1681 gtctgaagat gtatggtagt tttggcccca tcgatcaact gtacgatggt ggtgaagaag
     1741 tccacacccc cgtaaacgag acgcactttg ttgtggtgtg tatagcccgc gagatgacgg
     1801 gacgtcttct tcttccttgc catggtcgaa acaaaccaat taaggtagca ctattttttt
     1861 atttttttaa atattaaata attgttaact taatagacat atgaaagctt gcttacgctt
     1921 tgaaacctgg tgcctcgggc tcttgctcgt gccctgcttc tcttcagggc agaacgatta
     1981 tcaccttagt ggctatacgt acaacgatgc ccatgggagc atcaactcca acgttccttt
     2041 gaatgagatc aatccgcatg cctggcggca tttccataac ctgttcccgg gagccgatgg
     2101 agaatactgg ttcgcctccg gggagggata ccaggtttcg ttcatacaga aagggtatca
     2161 ctaccaggct tattttgacc agcgtggcgc ttagcgtcaa gactatttac ctggtcagat
     2221 tggtgaacgc tacgacactc cggactctaa ccgtttgcga tgggaaaatt gatgtcatcg
     2281 aagaactgac caatggaggc atccagccga cggccgggac atcagcggtc acgggcgacg
     2341 ggtcggcgcc tatcgctacc acaccgctta tttcgaaacg gtccgaccag tagccgtccg
     2401 cgttttacgt tcgagtcttt ccaattcttt cttcttgata acgagggtag cggtggcgac
     2461 atcggaacac gtaccgcaac gagccctgaa tttttccgaa tcataggtca ccaaggtggt
     2521 gcctaatttc cactccgtac catataagac ataatcattg gcggtataac cgctgccgag
     2581 gccgaaagca agatagctgt tccctttttc aaaactgacg agcagccggt cgttatcggt
     2641 tcccatgcaa aggccgggtg tcaatgaggg gatgactacc tcgttgatgg atttcccgtt
     2701 gctcagtttg accactccgt gcttgaccga gatatcctcg gacccgaggt tccggttgag
     2761 gatcagtttc tgatctacat agaattgcac gtctttcagg tcgatattcc ggacctccag
     2821 tttttgtttg aggtcccggg tgaagggggt gtaattggat agtttgttcg agctgccaca
     2881 actgtaaagc aaggttagtg aggtaaaacc aagggcgagg gtgaggatcc ttctcataat
     2941 atcaatattt gagtgggtat gaccattctg acacgcaata gttgaatttg ccggaagaaa
     3001 taagtgttat agaaatgtta aagcctgcaa cattccgcgg gattg
//
LOCUS       DASYSV010000480         4122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_737657, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000480 DASYSV010000000
VERSION     DASYSV010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737657"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..2647
                     /locus_tag="VGQ51_14790"
     CDS             176..2647
                     /locus_tag="VGQ51_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037899.1"
                     /translation="MLKSYFRIAWRILTRNRLYAFINICSLALGICGCIVIWLVAGYE
                     FSFDKFHPDAGRIYRVGQGGAKAEKKEPVVVPPMPDVIRRTIPGIETVATVFPLQQGS
                     EVMIPAGGSKPPVQFDTKVPGQNWVTSVIITDSNWFNIFHYQWMAGSPETSMGQPFSV
                     VLTEDRARQYFGPLSPQEMIGKEVIYQDSLPVHVTGIVKGWTAHTDLPYSDFITLSTI
                     PVSFLQDTRHMDDWVWHEGVGKWFWPECFVRLAQGAQAQKVEATLQPLGKQVVGSDSA
                     HPFLMQLQPLSDIHFNTDYTGDGVRKAHLPTLYALMAIAVFILLLGAVNFVNLSTAQS
                     LQRAREIGVRKVLGSGKTGLAVQFLTETGVITVFAVLVATLLVRPVLLYFREYIPEGV
                     SFYPLALPNLLFLAGIAIGTTFVAGFYPARVIASYLPVVSLKGGGTQKGDEKWWLRKS
                     LIVFQFSISLIFIIVALLMGRQISYMLKTDYGFKTDAIVSVLVKHDFWDTTSRIDVVE
                     QRFKQLPGAAEVIKEGSPPVGWGGWSMGVEYKGKQDVVQSATMDFGDEKYIPFYGMRL
                     VAGRNLHHSDTLREWVINETSARGLGFNHPADAVGQMLYLGRNAYPIVGIVADFHEGS
                     FKDPIRPVVIGHLPRWGSSLGIRMASAGKSTEEVKNLLTGMEKIYKGVYPNDPFSYVF
                     LDDSIANLYETEQKTASLVRSVMILAIFISCMGLFGLGLFTARRRAAEISIRKVLGAT
                     TGNIAMMLNKGFVFLVLLSLVIASPVAWWLAHRWLQDFAYRAPMAWWIFPVAGAGAVL
                     IALLTVSFQSIRAAMANPIKNLRTE"
     gene            2649..>4122
                     /locus_tag="VGQ51_14795"
     CDS             2649..>4122
                     /locus_tag="VGQ51_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037900.1"
                     /translation="MLRNYFIIAWRNLVKNKVYSAINILGLAVGMGVTLLIGLWVWDE
                     LSYNRNFEHYDRIVRVMENSTHGSETNTWNSVPIPLSGLLRTTYGSDFKRVSMASWNY
                     DHYLAYGDKQIKEPGMYVQADFIPMFSIRLLKGSAASLNEPYSMLLSVSAAKAIFGDE
                     DPMNKIIKVDNKSSLKVTGVFEDLPKNCEFNDTRILESWDGYVADNDWVKGSTTNWDN
                     NSFQIYAQLQDAAQLPTVAVRVKGGLDGHERKDKPQVVLHPMSKWRLHAEFKNGINTG
                     GAIQFVRMFGIIGVFVLLLACINFMNLSTARSERRAKEVGIRKAIGSLVRQLVGQFLS
                     ESLFITFLSFCLALLLVELGLPFFNGLADKQMGITWSSGWFWTICIAFVGITGLIAGS
                     YPAFYLSSFNSVTVLKGTFKAGRFASLPRKILVVLQFTVSVSLIIGTLVVFQQIQYAK
                     DRPIGYNRDGLLTIALSTPDLYTHYNSIRTDLLRTGAVADV"
BASE COUNT          896 a         1010 c         1232 g          984 t
ORIGIN      
        1 aaggtacgta aaaaaacgta cgttcaaaat attaccatga ttttttttga caggtgaccg
       61 gacacatttt gtatcgaaaa cggacagctg cggctgatgg cccggcacaa aatgctggga
      121 attaaaccct tgcagaacgg tacgtctttc gtggtctgca gcgaaaccgg agctcatgct
      181 aaagagttat ttccgcattg cctggcgcat tcttacgcga aacaggcttt atgcctttat
      241 taatatttgc agccttgcgc tcggcatttg cggttgcatc gtcatctggc tggtggccgg
      301 ctacgagttc agcttcgata aattccatcc cgatgccggc aggatatacc gtgtgggcca
      361 agggggggca aaggcggaga agaaggagcc tgtagtggta ccgccaatgc cggatgttat
      421 ccggcggacg atcccgggaa tagagacggt ggcgactgtc tttccgctcc agcaaggttc
      481 ggaggtgatg atcccggctg gaggctccaa accgccggtt caattcgata ccaaagtgcc
      541 gggtcagaac tgggtgacga gcgtcatcat cacggattcg aattggttca acatcttcca
      601 ttaccagtgg atggccggca gcccggagac ttcgatggga caaccctttt cggtagtcct
      661 gacggaagac cgggcccgcc aatatttcgg tccgctgtcg ccgcaagaga tgatcggaaa
      721 agaggtgatc taccaggatt ccctgcccgt ccatgttacg gggatcgtga aaggttggac
      781 ggcccatact gatcttcctt attcggactt tataacgctg tccacgatac ccgtcagttt
      841 tctccaggat acccgacata tggatgactg ggtatggcat gaaggagtag ggaagtggtt
      901 ctggccggag tgttttgtac ggctggcgca aggagcccag gcgcaaaaag tagaggcgac
      961 tttgcaaccg ttgggtaaac aggtggtcgg gtccgactcg gcgcatcctt tcctgatgca
     1021 gttgcagcca ttgtcggaca tccatttcaa tacggattac actggggacg gcgtgcgcaa
     1081 ggcgcatctc ccgaccctgt atgccctcat ggctattgcg gtgtttattt tgctgctcgg
     1141 agcggtgaac tttgtcaacc tgtctacggc gcagtcgctg cagcgggcca gggagatcgg
     1201 agtacgtaag gtattgggca gcgggaaaac ggggctggcg gtgcagttcc tcaccgagac
     1261 aggcgttatt acggtcttcg ccgtgctggt ggcaaccctg cttgtccgac ccgtcctgtt
     1321 gtactttcgg gaatacatac ccgaaggcgt gtcattttac ccactggcac tgccgaacct
     1381 gctgtttttg gccgggatcg cgatcggcac gacgttcgtg gccggattct accctgcccg
     1441 ggtgatcgcg agctatcttc cggtggtcag tttgaaggga ggcggcacgc agaaaggaga
     1501 cgaaaaatgg tggttgcgga aaagcctgat cgtttttcaa ttcagcatct cgctcatttt
     1561 tatcatagtg gccctgctga tgggtcgcca gatcagctac atgctgaaga ccgattacgg
     1621 attcaagacc gatgcgatcg tatcggtcct ggtgaaacat gacttttggg acaccacttc
     1681 caggatcgac gtagtggagc agcggttcaa acaactaccc ggggcagccg aagtgataaa
     1741 agagggatcg cctcccgtgg gttggggagg ttggagcatg ggtgtcgaat acaaagggaa
     1801 acaggatgtg gttcagagcg ccacgatgga tttcggcgat gaaaagtaca ttccctttta
     1861 tgggatgcgg ctggttgccg gcagaaatct gcatcatagc gataccctga gggaatgggt
     1921 gatcaacgag acgtcggcga gaggtttggg ttttaaccac ccggccgatg ccgtcggcca
     1981 aatgctctat ctcggccgga atgcttatcc tatcgtcggc atcgtcgccg attttcatga
     2041 agggtcgttc aaggacccga tccggcctgt cgtgatcggt catctgccgc ggtgggggag
     2101 ttcgctcggg atccggatgg cgagtgcggg gaagagcacg gaggaggtga agaatctgct
     2161 cacgggcatg gaaaaaattt acaaaggggt ttatccgaac gatcctttca gctatgtctt
     2221 cctggatgat tccatcgcta atttgtatga gacggagcaa aaaacggcgt cgctggtacg
     2281 atcggtgatg atcctggcga tcttcatctc ctgtatgggt ctcttcgggc tgggcttgtt
     2341 cacggcgcgg agaagagcgg cagagatcag catccggaag gtgctcgggg ccacgaccgg
     2401 aaatatagcg atgatgctca acaaagggtt tgttttcctg gtcctgttgt cgctggtcat
     2461 cgcgtcgccg gtggcatggt ggcttgcgca ccggtggctg caggattttg cctatcgcgc
     2521 gccgatggcg tggtggatat tcccggtggc tggcgccggg gccgtactga tcgctttatt
     2581 gaccgtcagc tttcaatcta tccgcgccgc catggccaac ccaattaaaa atttaagaac
     2641 cgaataatat gctccgaaac tattttatca tcgcctggcg caatctggtc aagaacaaag
     2701 tctactcggc catcaatatc ctggggctgg ctgtcggaat gggggttacc ctgctgatcg
     2761 gtctctgggt ctgggacgag ctttcctaca accggaattt cgaacattat gaccggatcg
     2821 tacgggtgat ggaaaattcc acgcatggaa gtgagaccaa cacctggaat tctgtgccga
     2881 tcccgttgtc gggtctgctg cgaacgacct acggttcgga tttcaagcgc gtttccatgg
     2941 cgtcatggaa ttatgatcac tacctcgcct acggagacaa acagatcaag gagcccggga
     3001 tgtatgttca ggcggacttc atacccatgt tctccatccg gctgctcaag ggatcggcgg
     3061 cttcgctgaa cgaaccgtat tcgatgctat tgagcgtgtc ggcggcgaag gcgatattcg
     3121 gcgacgagga tcccatgaac aagatcataa aagtggataa taagagcagc ctgaaggtaa
     3181 cgggcgtctt cgaggacctg ccgaagaatt gcgagttcaa tgatacacgc atcctggaat
     3241 cctgggatgg ttacgtggcc gacaacgatt gggtcaaggg ttcgacgacc aattgggata
     3301 acaactcttt ccagatctat gcacagttgc aggatgccgc gcagctaccc accgtcgcgg
     3361 tgagggtgaa gggcggattg gatgggcatg agcggaaaga caaaccgcag gtggtcctgc
     3421 atcctatgag caaatggcga cttcacgccg aatttaaaaa cgggatcaat accggtgggg
     3481 cgatccagtt cgtccggatg ttcggtatca tcggcgtatt cgtgcttttg ctggcctgta
     3541 tcaattttat gaatctcagc accgcccgat ccgagcggcg tgcgaaagag gtcggtatcc
     3601 gcaaggctat cggatcgctg gtacggcagc tggtgggaca attcctgagc gaatcgttgt
     3661 tcattacttt tttatctttc tgtcttgcgc tgctgctggt ggaattgggg ctgccctttt
     3721 tcaacgggct ggccgataag caaatgggaa taacgtggag cagcggatgg ttctggacca
     3781 tttgcatcgc attcgtgggg atcaccgggc tgatcgcggg cagctatccc gccttttatc
     3841 tttcatcgtt taattcggtg actgtgctga agggtacgtt caaagccggg cggttcgctt
     3901 cgctgccgag gaagatattg gtggtcttgc aattcaccgt gtcggtatcg ctgatcattg
     3961 gaacgctggt ggtctttcag caaatacagt atgcaaagga ccggcccatc ggctataacc
     4021 gggatggctt gctgacgatc gcgttgagca cgccggacct gtacacccat tacaacagta
     4081 tccgtaccga cttgctgcgt accggcgccg ttgccgatgt gg
//
LOCUS       DASYSV010000481         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1251202, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000481 DASYSV010000000
VERSION     DASYSV010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251202"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VGQ51_14800"
     CDS             complement(<1..181)
                     /locus_tag="VGQ51_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037901.1"
                     /translation="MTRIQSLFLLLSPLAASGQAPTRDTVPHAPKAATLQTATVTGHT
                     PLVQRKKDRVIINVEV"
     gene            355..2775
                     /locus_tag="VGQ51_14805"
     CDS             355..2775
                     /locus_tag="VGQ51_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037902.1"
                     /translation="MLKNYITTAVRNLVKNKFYSLLNISGLAVGLATCMLIWLYVTDE
                     VSYDRYNTNATRIYRVNSEIKFGGNYIDLAVATPQMGPAMAKELPEVEQYTRLMWHGS
                     IHIRKGSENMQEDRVAFADSTLFRVFTLPMISGDPATALKDPHSMVITERAARKYFKD
                     VNVVGRSFLVNDSVPYKITGVIRDIPSQSHFNFDYFIAMAEFAASRDNSWLGTENFPT
                     YVLVRKGTNVKKLEAGMNDLLDRYLGPQLSAAVNMSLDQFKKGGAFIRGSLTPLTQIH
                     LYSNRTGEIEGGGSIQYVYIFSAIAIFILLIACVNFMNLSTARSANRAKEVGIRKVMG
                     SLKGNLIAQFLSESIVIAFTALALGLLIAYAALPSFDNLAGKQIGASALFGAGTLVFL
                     FLLVLVVGVLAGSYPAFFLSAFRPIDVLKGKLTGGFKRSWLRNGLVVFQFSLSIVLII
                     GTGVVYNQLRYMRNKDIGFDRSQVMVIEHTDVLGNRARAFRNELARLSGVRATTVSPY
                     LPVGYYRSGNTFFDSPARDPKAAMNMQVWGVDADYIPTLGMKMLVGRNFSPAFPSDSD
                     GLILNEAAYRFLGSPDITRKKLYAFVKPTETKPYHIVGVVKNFNFSSMREVITPLCLE
                     LGNENAAVAVRIDVGAASEVTAGIKELWQKMAAGQPFVYSFMDDSFNRLYTAEVRTGQ
                     IAFVFSLLAILIACLGLFGLVTFAVEQRTREIGIRKVLGADVTGIVAMIGKDFLRLVL
                     IASLIAFPVAAWVMSGWLQGFAYRIGLQWWVFALAGVIAVFIALCTVGSRAIHAAMAN
                     PVDSLRSE"
BASE COUNT          708 a          753 c         1051 g          845 t
ORIGIN      
        1 agacttccac gttgatgatg acgcggtctt tttttcgctg gacgagtggg gtatggccgg
       61 tgactgtcgc ggtctgcagg gtcgcggcct taggtgcgtg gggtacggtg tcccgcgtcg
      121 gggcctgacc agacgctgct aagggagata ataagaggaa cagactctgg atcctggtca
      181 taaagtcaaa gatgaattac attttctgaa atgtgccgcc gctgctgtaa tagtcagacg
      241 gaggcgcaca ccaggtgtat cgatatcgga cggttttggc cccttcggta cccgtaatat
      301 gctgaaaatc tgtggcctgc acaatcggca ttcttttaga cgcggtcgtg ggttatgctg
      361 aaaaattaca ttacgactgc cgtccgcaac ctcgtcaaga acaagttcta cagccttctc
      421 aatatcagcg ggcttgcggt aggcctggcg acctgcatgc tcatctggct gtatgtcacc
      481 gacgaggtca gctatgaccg ctataacact aatgctaccc gtatctaccg ggtaaactcg
      541 gagatcaagt tcggagggaa ctacatcgac ctggccgtcg ccaccccgca gatggggcct
      601 gcgatggcga aggaattgcc ggaagtggag cagtatacgc ggctgatgtg gcatggaagc
      661 attcatatcc ggaaagggag tgagaacatg caggaggaca gggtagcgtt tgcggactcg
      721 acgcttttcc gggtgttcac gctgccgatg atctcgggtg atccggcgac ggcgctcaag
      781 gacccgcatt cgatggtcat tacggagagg gctgcccgca aatattttaa ggacgtcaat
      841 gtggtgggaa ggagtttttt ggtcaatgat tcggtcccct ataagatcac cggtgtcatc
      901 cgggatatcc caagccagtc gcatttcaat ttcgactatt ttatcgccat ggccgaattt
      961 gccgccagca gagataacag ctggcttgga acggagaatt tccccacata tgttcttgtg
     1021 cgcaaaggga cgaatgttaa aaagctggaa gccgggatga atgatctgtt ggaccggtat
     1081 ctgggtccgc agctgagcgc ggcggtgaat atgagtctcg atcaattcaa gaaaggaggg
     1141 gcctttatcc ggggcagtct tacgcctctg acccagatcc atctctattc caaccggaca
     1201 ggggagatcg aaggcggggg cagtatacag tatgtctata tctttagcgc catcgcgata
     1261 ttcatcctgc tgatcgcgtg tgtgaatttc atgaaccttt ccactgcccg ctccgccaat
     1321 cgggccaaag aggtcggtat ccgaaaggta atgggctcgc tgaaagggaa cctgatcgca
     1381 caattcctgt cggaatccat cgtgatcgcg tttacggcgc tggcgctcgg tctgctgatc
     1441 gcgtatgcgg cattgccatc tttcgataac ctggcaggta aacagatagg agcctctgcc
     1501 ttattcggtg ccgggacgct ggtcttcctg tttttgctgg tgcttgtggt cggcgtgctg
     1561 gcggggagct atcctgcctt ttttctttcg gcattccggc ctatcgacgt gttaaagggg
     1621 aaattgacgg gtgggttcaa gcgatcgtgg ctgaggaacg ggctggtggt ttttcagttt
     1681 agcttatcga tcgtgctgat catcgggacg ggagtggtat ataaccagct gcgctatatg
     1741 cggaataagg atatcgggtt tgaccggagc caggtgatgg tgatcgagca cacggatgtg
     1801 ctgggtaacc gggcccgcgc tttccgcaat gaattggcac gtttgagcgg agtacgggcc
     1861 accacggtga gtccctatct tccggtgggg tattaccgga gtgggaatac gttcttcgac
     1921 tcacccgcca gggaccctaa ggctgcgatg aacatgcagg tgtggggggt ggatgcggac
     1981 tatatcccga cgcttggaat gaagatgttg gtgggaagga atttctcgcc tgccttcccc
     2041 agcgactcgg atgggcttat cctgaatgaa gcggcttaca ggttcctcgg ctcgccggat
     2101 attacgcgca aaaaattgta tgcgtttgtc aagcccacgg agacgaaacc ttatcatatc
     2161 gtcggggtgg tgaaaaattt caatttcagt tcgatgaggg aggtgatcac accgttgtgt
     2221 ctcgaattgg gtaatgagaa cgcggctgta gcagtccgga tcgatgtcgg ggccgcctcg
     2281 gaggtgactg ccggtataaa ggagttgtgg cagaagatgg ctgccgggca gccgttcgtg
     2341 tatagtttta tggacgactc tttcaacagg ctttatacgg ctgaggtcag gacgggtcag
     2401 atcgcgtttg ttttttcgct gctggcgata ttgatcgcgt gtttgggact gttcggtctg
     2461 gtgacgtttg cggtagaaca gcggacgagg gagatcggga tacggaaggt attgggggct
     2521 gatgtgacgg gtatcgttgc gatgatcggg aaagactttt tgagattggt gctgatcgcc
     2581 tcgctgatcg cgtttcctgt ggccgcgtgg gttatgagcg ggtggttgca ggggtttgct
     2641 taccggatcg ggttgcaatg gtgggtgttt gcgctggcgg gcgttattgc ggtatttata
     2701 gcgttgtgta cggtggggtc gagggctatt catgcggcga tggcgaatcc tgtggatagt
     2761 ttgcgatcgg agtgacgccg atgcggcggc tgggcggctg gggaggggaa cggcgagatg
     2821 gaggcgggtg gccgtggaga tgagcgcgtg agatggaggc gggtggccgt ggaggggttg
     2881 gcatgctcat tgcagactaa acaataaggg ttcacgctta attgcctgta ctttgccctc
     2941 atacagataa ttttatgacg agcatttcgt tttggatata ggccatatgg tagaaccgag
     3001 atatggtgag cactgcccgc gtcaaacatg ctgatccgtt tacaatatga ttacggcgtc
     3061 cacgggcagc gatccatatc caccgggaaa acgtcaaaca agctgatcaa atgatatttt
     3121 tatttgatcc gtctcccgaa gaaccttcgc gaacccctct taccgggctg tagcagccga
     3181 atttgaacct ccgggtattt gcccggaggt ttttttgttt ggggagggga cgattgccgg
     3241 gcggtgcaaa acgattatat ttaaggaaac caactgatat gaagttctta tcacttgccc
     3301 cttttgtccc ttccggtaat aagcacgacg aatccaagca gctttttcag gagttgg
//
LOCUS       DASYSV010000482        12560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_98728, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000482 DASYSV010000000
VERSION     DASYSV010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12560
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98728"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3157
                     /locus_tag="VGQ51_14810"
     CDS             <1..3157
                     /locus_tag="VGQ51_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037903.1"
                     /translation="LGEFAANQALLDLPQWLTDGYIEYVSENWNTVLDDQLKSAMLSG
                     RYNNFYQFAYERPNLAGHAFWYYLGEKYKKENVTYFLYLSRVYRNLNNASQRICKKKF
                     KEVLKDFMAEQEDKYEKDIRGRRNFPKGQVSVTEDVGPNKDYFHFSPNPTPKSQTYAV
                     VQYKHGRYKVILYENYINEKVLLSVGARSLEEQINPHYPLIAWDPKGTRLTVMYWSRG
                     HVRMFTYDLAKRYKTQFVETPGEEGKPGNTLEAAKGEAQFAEFHQFPDFQQVQDMKYM
                     LNSHTLLLSAVLNGQSDIFTYDLDKGTTKQITNDIYDDLDATFVAFPGKTGIIFSSDR
                     PTGMAPSKDTVLPSNYHYNIFLVDNWNDNEGKQVSQLTHLRYGNARYPMQYNVSHFTF
                     VSDENGIANRYAGFFTTRRAGLDTVYKVGDELLHNPDPATLDSALTAQGKKQPDTSYV
                     FAITNDSSYVFPITNYQSSLVETKSAGDNGQVSEVRQEGDVKFLYKLKVDEQALQRRN
                     VNARLTDYRKKTVAEDRISGANLLLPVAPQKQDTGRRQPTGDFFESEFDNKKDTSHRP
                     ANNPNAAGQPAPTGVNPFALRPTNQAAHGEETTLHKAKLFDYKLKFSVDNFTAGFNND
                     VLISKYQPYTGSLPINLSGQDAFSGMLKASVFDLFEDIRFTGAIRLPFFGSGETQSQV
                     STPNSAVFAPGNSSFFDGAGEYFARIDYLKRLFDYSLIYYRSTQTGTYQDPALGSEYP
                     YDAKAYSNIWQLIIKYPFDKVRSLRLSVGYRMDKLQIRPDITGFPPIPPIDSVGLTAP
                     ALNKQNYGLVHLEYVYDNTLLRATDIWNGLRYKFYLDVDPQLNGGEGTGAGRYTYNFG
                     FDARNYIPIFRNFIWALRAAGDFSWGNKKIVYYLGGTDGWLFPKADNLPQPQDPTYAF
                     QSLAVNLRGFNQNIANGNNDVVINSELRLPVFTTLFNKPINNAFLRNFQIVQFVDLGT
                     AWNGRYNGLKRPQVTYQSDSSSNFTVQIKAGGIGPFAGGYGFGARSTLLGYFLRFDAG
                     WQMNTFFGNKPILNVSMGVDF"
     gene            complement(3332..3841)
                     /locus_tag="VGQ51_14815"
     CDS             complement(3332..3841)
                     /locus_tag="VGQ51_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV9037904.1"
                     /translation="MVATSKLQEFFIDQLQDIYWAEQKLISTLPKLEEAASSVELRQA
                     FNSHLQETKNHVSRVEKVFELIGVPAHAKKCHAISGIVEEGEEIIDETEENTAQRDVG
                     LIFAGQKAEHYEIATYGGLVTLARTLGFVDAAEILSVTLAEEKKADNLLTRIAENGIN
                     VQAKEEVNE"
     gene            complement(3866..5017)
                     /locus_tag="VGQ51_14820"
     CDS             complement(3866..5017)
                     /locus_tag="VGQ51_14820"
                     /inference="COORDINATES: protein motif:HMM:NF042563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6688 family protein"
                     /protein_id="HEV9037905.1"
                     /translation="MSEQIMELFLLAPLMVVSVVLGWFATLPPVMEYLSWKIHRGLYP
                     VWSRYWFAVAVIVPPFVGMFSDTEGAGERYAPCLSPEHRLSVMVLIACSFAAFIYSLY
                     RKRLAPPLMEVLINCLLLSGIVVAIALMLQGGEWIEGMVMCLPFVLQSMLLMADNHRM
                     VIERLGEEEDDADSRWAGFCRRIILLPAWKKFPLFLLLCIPVVCLVSAVLLLFGQKPD
                     SVIRAFTDAYKGDYFRVFGHGREPDLGAHYLCSVAAKGHPRLVRPIRMGERGGKPIKC
                     NRQLLVSNAFEELLEQRLPGLHRPVRLLYNKIGAGIHRYYGVFDHAWVSDLIYILMKP
                     LEWGFVAVLYLSDRRPEDRIARQYLRKEDAAKCVEEWGMRWVGMRGTII"
     gene            complement(5014..6465)
                     /gene="creD"
                     /locus_tag="VGQ51_14825"
     CDS             complement(5014..6465)
                     /gene="creD"
                     /locus_tag="VGQ51_14825"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEV9037906.1"
                     /translation="MEIKTPGLWNNFRTLFKSALVGALILLLLIPTAFIQNLVSERQD
                     RQHQAVAEIGSRWAGPQTVTGPVIGIPYYDTVSDNAGRQSIKRWAYFLPDKLDIHASI
                     VPQRRYRGIYEVIVYTTQMDIRGSYKSLPLEELGLSASDMLWKEARLFLDISDIQGLN
                     EDVVMKIGTTTGGLVGAGGATAGTGGLAGAGGTTAGTGLAGAGGASGFSAAIPMVTGG
                     RSTEQFGNALSAPLPDGLAMLAWKGNTDGNPALEFATTVQMKGSGDLLFVPVGKETKV
                     SASSPWSSPSFTGSTLPDVRTVGDSGFVADWKLIALHRKFPQQWKQGTYDLASAGFGV
                     DLIVPVDAYQQTTRSVKYAILIILLTFTAFLLIEWIYNLPMGALQYVLVGFALCIFYT
                     LLLSLSEYLGFNAAYGLAAAATIGLIGWYVGVILHSRKVALFVTFLLVVQYGFVFILI
                     QLQDYALLMGSLGLFVTLAIVMQASRKIKWQTA"
     gene            complement(6483..7280)
                     /locus_tag="VGQ51_14830"
     CDS             complement(6483..7280)
                     /locus_tag="VGQ51_14830"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEV9037907.1"
                     /translation="MISYPGQAGGTWRRWFLRSETDRLLTCSMCFSCGLVILRMIHAH
                     SLLFVFMIWNLFLAYIPYVLSSWLSRRLEGVESWPNRRLDHPLTKSLSRPISSRPLFI
                     VAVCLIWLLFFPNAFYMLTDMFHLHDSRNPRVPEWFDLAMIFSFAWNGLLLGVLSLRQ
                     MERLLENKRLSKAIFGPRLQVPLPYPTAVFVYPVIALSALGVYTGRYLRYNSWDILTD
                     PFQLMEDIATMVIHPLSNRPAWDMILCYAILLSFVYVMLKKLSRALN"
     gene            complement(7277..7645)
                     /locus_tag="VGQ51_14835"
     CDS             complement(7277..7645)
                     /locus_tag="VGQ51_14835"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV9037908.1"
                     /translation="MDTEKFSVRARIKSFHYAIAGLRRFVNREHNARIHLVATILVII
                     VARVLEVSATEAAILAAVIGMVWVAEILNTCVEKLADHITKERHPEIEIIKDLAAGAV
                     LVAAIVAIAAGSFIFIPKLL"
     gene            complement(7657..9111)
                     /locus_tag="VGQ51_14840"
     CDS             complement(7657..9111)
                     /locus_tag="VGQ51_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV9037909.1"
                     /translation="MKIKMILPALTEAKSPFWRPIKYSLFPPLGLATLAAYCGPDDDI
                     DLQDEHVEELRSDDEPDLVVIQVYITNAFRAYAIADHYRSKGAYVCLGGLHVTSLPDE
                     AEVHADSIFIGPGEDSWPRFLKDFREGSPGRVYRNTERDITEIPPIRRDLIKRERYLV
                     PNSIVVSRGCPHHCDFCYKDAFFEGGRSFYTQRVDAALAEIDRLPGRHLYFLDDHLLG
                     QQRFATALFDGMKGMNRVFQGASTIDAILRGDLIERAAEAGLRSVFVGFETLSEGNLV
                     QSNKRQNIGRDYEEAIRRLHSLGIMINGSFVFGLDADDPGVFHRTVEWAVRNSLTTCT
                     FHILTPYPGTRLFKDMEAQGRILHRNWDQYDTRQVVYKTVGLTPAELKKGYDDAYRSF
                     YSWSNIWRASLGHDSLKYKIKHFSYAGGWKKFEPMWNFLIKTQGLNKMLPLLEAILSR
                     VKRTGDFTPGGPGSRGSDGDRKPGLPLSPFPAIA"
     gene            complement(9116..9481)
                     /locus_tag="VGQ51_14845"
     CDS             complement(9116..9481)
                     /locus_tag="VGQ51_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037910.1"
                     /translation="MNNQSITRLIFLRHLASGLVGIALMLPAIFFLLTAVKRVCFGSR
                     WMYGYMAQQTQLMIGCLVVAVVFNALALVTVRIVPGRIGPDVELGFRRSWLNVAVVLQ
                     GALFLVGLVAYLFIEYLRY"
     gene            complement(9698..10003)
                     /locus_tag="VGQ51_14850"
     CDS             complement(9698..10003)
                     /locus_tag="VGQ51_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV9037911.1"
                     /translation="MKNPIGNLNKIFDSRIRMGVMSILLVNEEVSFNDLKQLLELTDG
                     NLASHLINLEENGFIKVHKGFIGRKTNTTYSITKAGEKAFKEHLEALESMIKGVSKG"
     gene            complement(10000..10641)
                     /locus_tag="VGQ51_14855"
     CDS             complement(10000..10641)
                     /locus_tag="VGQ51_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037912.1"
                     /translation="MSNQSPAEQSLEALQDIKRMMERSSRFISLSGLSGVSAGVCALI
                     GAWVAHGWIQDYYERYLSVHGYVATERYDFKWKLIVLAGVVLAAALVSATLFTWRKAR
                     RSRLPILDHASKKLAINMMIPLVAGGLFVIGLLYNADWIYVAPACLIFYGLALVNASK
                     YTLTDIRYLGLLEIALGCVSMYFPHEGLYFWTAGFGILHIIYGLIMWWKYERT"
     gene            10782..11222
                     /locus_tag="VGQ51_14860"
     CDS             10782..11222
                     /locus_tag="VGQ51_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037913.1"
                     /translation="MENDQQESLIQMHLDYDGGNILRETVRWSRFLSIVGIIGLALCL
                     LIFVLAGSAIVAAFSRLSPGLEGLAGLGGVILLIIMIAFFAISGFLVYMLFRFSKMIR
                     RGIDHQDQATFSEGMKSLKTYFIISGILSALLLLFNALSLIQLF"
     gene            11235..11714
                     /locus_tag="VGQ51_14865"
     CDS             11235..11714
                     /locus_tag="VGQ51_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037914.1"
                     /translation="MDSQPTSQTTNSLDNFFNVSLDTATRSQIRQAAVWARVTALCAF
                     GAYAITLIVAFFGRVAGLDDSAIGLEVNDSFRINRIIGALITAVIGVIINLFLYRFAQ
                     STIRGIDAMDNVLTNDGFNQLRRYFKILGILLIVVFSLVILGILIGIGVGLSRTSSY"
     gene            complement(11969..>12560)
                     /locus_tag="VGQ51_14870"
     CDS             complement(11969..>12560)
                     /locus_tag="VGQ51_14870"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEV9037915.1"
                     /translation="VICDYLSREESRKLYEPGTEFQIAKIEMVANTGTYIDVPFHRFG
                     DGHDLSEVGLERVAELPGIVVRIPYSRGLAVRVSDLRGFEVRGRAVLIHTGWDEHWNT
                     PVYFSGHPFLTLDAAEYLRDSGARLVGIDSHNIDDTAGRSRPVHTTLLGAGIPIVEHL
                     CELRLLPDEGFVFSAVPPKFKGVGTFPVRAMARVGR"
BASE COUNT         3074 a         4008 c         2815 g         2663 t
ORIGIN      
        1 cctcggcgaa ttcgcggcca accaggcact gctcgacctg ccgcaatggc tcaccgacgg
       61 ctatatcgaa tatgtctccg aaaactggaa tacggtgctg gacgaccagc tcaaatcggc
      121 catgctcagc gggcgatata ataattttta tcagtttgcg tacgaacgcc ccaatttagc
      181 gggccatgcc ttctggtact acctggggga aaaatataaa aaagagaacg tcacctattt
      241 cctctatctg tcccgcgtct accggaacct caacaacgcc tcccaacgca tctgcaagaa
      301 aaaattcaag gaggtcctca aggacttcat ggccgaacag gaggataaat acgaaaaaga
      361 catccgcggc cgccgcaact tcccgaaagg acaggtctcc gtcacagaag acgtcggtcc
      421 caataaagac tatttccact tctcgcccaa ccccactccg aaaagccaga cctatgcggt
      481 agtgcagtac aaacacggca ggtacaaggt catcctttac gaaaattata tcaacgaaaa
      541 ggtattgctg agcgtcggcg cccgttcgct cgaagaacag atcaatcccc attatcccct
      601 catcgcctgg gatcccaagg gtacccgcct gaccgttatg tactggtccc gcggacatgt
      661 caggatgttc acctacgatc tggcgaaaag atacaagacg caattcgtcg agacacccgg
      721 cgaagaagga aaacccggca acaccctcga agccgcaaaa ggcgaagccc aattcgccga
      781 attccatcag tttcccgact tccagcaggt acaggacatg aaatacatgc tcaacagcca
      841 caccctcctg cttagcgccg ttctcaacgg ccagtccgat atctttactt acgatctcga
      901 caaaggcacc accaaacaga taaccaacga catctacgac gacctcgacg ccaccttcgt
      961 cgccttcccg ggcaagaccg gcatcatctt ctcctccgat cgtcccacag gtatggcgcc
     1021 gagtaaggat accgtgttgc cctccaacta tcactataac atcttcctcg tcgacaactg
     1081 gaatgacaac gaaggcaaac aggtctccca actgacccac ctgagatacg gcaatgcccg
     1141 ttatccgatg caatacaacg tgagtcactt cacattcgtc agcgacgaga acggtatcgc
     1201 caaccgttat gccggtttct tcaccacccg ccgcgccggt ctcgatacgg tgtataaggt
     1261 gggtgacgag ttactgcata accccgaccc cgcgaccctc gactcagccc tcacggctca
     1321 gggcaaaaaa caaccggata cgtcatacgt gtttgccatc accaacgact cgtcctatgt
     1381 cttcccgatc accaattacc agagcagcct cgtagagacc aaaagcgccg gcgacaacgg
     1441 ccaggtcagc gaggtgagac aggaaggcga tgttaaattc ctctataagc tcaaggttga
     1501 cgagcaggcc cttcaacgcc gtaatgtcaa cgctcggttg accgactatc ggaagaaaac
     1561 cgtggccgaa gaccgcattt cgggcgccaa cttgctgcta ccggtggctc cgcaaaaaca
     1621 ggataccgga cggcgtcagc ccaccggcga tttcttcgag agcgaattcg acaataaaaa
     1681 agatacaagc caccgtccgg caaacaatcc caatgcggcc ggccaacccg cccccaccgg
     1741 cgtcaatccc ttcgcactca gacccactaa tcaggccgct catggcgagg aaacgaccct
     1801 gcataaagca aagctcttcg actacaagct gaaattttcg gtggataatt tcaccgccgg
     1861 cttcaataac gacgtgctga tcagtaaata ccagccgtat accggctcgc tgcccatcaa
     1921 tctcagcggc caggacgcct tcagcggtat gctcaaagcc tctgttttcg atctgtttga
     1981 agatatccga ttcaccggcg ccatccgcct gcccttcttc ggcagcggtg agacccaaag
     2041 ccaggtctcc acacccaatt ccgccgtctt cgcgccgggc aactcttcat tcttcgacgg
     2101 ggcaggtgaa tatttcgccc ggatcgatta tctcaaacgt cttttcgact attcactgat
     2161 ctattaccgg tcgacccaaa cgggaactta tcaggatccc gcactgggct ccgaataccc
     2221 atacgatgcc aaggcctata gcaatatctg gcagctcatc atcaaatatc ccttcgacaa
     2281 agtgaggagc ctgcggttgt ccgtcggcta ccggatggat aagttacaga tccgcccgga
     2341 cattaccggc ttcccgccga ttccgccgat cgactccgtc ggactgaccg cacccgcatt
     2401 gaacaagcag aactatggac tggtacacct cgaatatgtg tatgacaata ccctgctccg
     2461 cgcaacggat atctggaacg gcctcaggta taaattctat ctggatgtag acccgcaatt
     2521 gaatggcggc gaaggcaccg gagcagggag gtatacctac aatttcggtt tcgatgcacg
     2581 caactacata cccatctttc gcaacttcat ctgggccctg cgcgcggcag gagatttctc
     2641 atggggtaac aaaaagatcg tctactatct cggcggaacg gacggatggc tcttccccaa
     2701 agcggacaat cttccacaac cacaggaccc cacctatgcc tttcaatcgc tggccgtcaa
     2761 cctgcgtggc ttcaaccaga acatcgcaaa cggcaacaat gacgtcgtca tcaacagcga
     2821 actgcgtctg cccgttttca ccaccctgtt taataaaccc atcaacaacg ccttcctgcg
     2881 caacttccag atcgtacagt ttgtcgatct gggcaccgcc tggaatggtc gctataacgg
     2941 actcaaacgt ccccaggtca cctatcaaag cgattcttcg tccaatttca cggtccagat
     3001 caaggccggc ggcatcggac cctttgcagg cggctacggg ttcggcgcca gaagcaccct
     3061 gctggggtat ttcctgcgtt tcgacgcggg ctggcagatg aatacgttct ttgggaataa
     3121 gccaatactc aatgtctcga tgggtgtaga cttctagcac agagttgcac agaatggcgc
     3181 gctggtcccg tgaggccggt cccgtgaggc gcgcaggccc tgtgaggccg gtcctcctgg
     3241 ccggccgatg cgggaaaacc tcctctggag aaataaaaaa agccggtaag cacttagtct
     3301 taccggcatc gcacaaatag ccccgcgtca cttattcatt tacttcttct ttggcctgta
     3361 cgttgatacc attctcagct atgcgggtca gcagattatc tgctttcttc tcttcagcca
     3421 gggtaacgct caggatctcc gcggcatcca caaaacccag ggtccttgcc agtgtgacca
     3481 gaccaccata ggtggcgatc tcataatgct ccgccttctg ccccgcgaag atgagaccca
     3541 cgtcgcgttg agcggtgttc tcctccgttt catcaatgat ctcttcaccc tcctctacga
     3601 tcccggatat ggcgtgacac ttctttgcat gcgccggcac tccgatcaat tcgaaaacct
     3661 tctccactct cgacacatgg ttcttcgttt cctgcagatg actgttgaag gcttgtctca
     3721 actcgaccga gctggccgct tcttccagtt tcggaagcgt actgatgagc ttttgctcgg
     3781 cccaatagat atcctgcaac tggtcgataa aaaattcctg caatttcgaa gtagccacca
     3841 tataggacct ctttgactat acttcttaaa ttatcgtgcc acgcatccca acccaccgca
     3901 ttccccactc ctccacacac ttagccgcat cctccttcct caaatattgc cgcgcgatcc
     3961 ggtcctccgg ccgcctgtcg ctcaaataca acaccgccac aaatccccat tccaacggct
     4021 tcatcaaaat gtagatcaga tccgacaccc atgcgtgatc gaatacccca taataccggt
     4081 gtatccccgc cccgatctta ttataaagga gcctcaccgg ccggtgcaat cccggcaacc
     4141 gctgttccag taattcctca aaagcattcg acaccaacag ctgtcggtta cacttgatcg
     4201 gcttgcctcc tctttccccc atccttatcg gccgcaccaa ccgcggatgt cctttcgccg
     4261 ccacggaaca tagataatgc gcgcccaggt ctggctcccg gccatgacca aagacccgaa
     4321 aataatcgcc cttatacgca tcggtgaaag ccctgatcac cgaatccggt ttctgaccga
     4381 aaagcaacaa taccgcggat actagacata ccaccgggat acacaacaac aagaatagcg
     4441 ggaacttttt ccaggccggc aagagaatga tcctgcgaca gaaccctgcc catctgctgt
     4501 cggcatcgtc ttcctcctca cccagccgct ctataaccat ccgatggtta tccgccatca
     4561 gtaacatcga ttgcagcaca aatggcaggc acattaccat gccctctatc cattcccctc
     4621 cctgtagcat cagggcgatg gcaaccacta tgccggaaag taacagacaa ttgatgagca
     4681 cctccataag cggcggtgcg agacgcttgc gatacaacga ataaataaag gcggcgaagg
     4741 aacaagctat cagcaccatc accgacaacc ggtgctccgg cgataagcag ggagcatacc
     4801 gttcccccgc accctctgtg tcgctaaaca ttcccacaaa gggcggaacg atgactgcca
     4861 ctgcaaacca atacctcgac cacacgggat acagcccccg gtgtatcttc cagctcaagt
     4921 attccattac cggcggcagc gtcgcaaacc aacccaggac aaccgagacg accataagag
     4981 gagcaagcag gaacaactcc atgatctgct cgctcatgcc gtctgccact tgatctttct
     5041 cgacgcctgc atgacgatcg ccagcgtgac gaacaacccc aggctaccca tcagcaacgc
     5101 ataatcctgc aattggatca ggatgaacac gaacccgtat tgcacgacaa ggaggaacgt
     5161 aacgaacaaa gcaaccttcc tcgaatgaag aataacgccg acataccatc cgatcaatcc
     5221 aatggtcgcc gcggcagcca gtccataggc cgcgttgaat cccaggtatt cggacagcga
     5281 gagcagcagg gtataaaaga tacacaacgc gaatcctacc agcacgtatt gcaaggcccc
     5341 catcggcagg ttatagatcc attctatcag caggaaggcg gtgaacgtca gcaggatgat
     5401 caggatcgca tatttgacgc tgcgcgtcgt ctgctgatag gcatctaccg gaacgatcag
     5461 gtccacgccg aaccctgccg atgcgaggtc atatgtaccc tgcttccatt gctgcgggaa
     5521 tttgcgatgc aacgcgatca gcttccagtc ggcgacaaaa ccactgtccc ccacagtccg
     5581 cacatcaggc agagtactgc cggtaaagct gggactcgac cacggcgacg acgccgaaac
     5641 ctttgtctcc tttccgacag ggacgaacag caggtcgccc gaacctttca tctgcaccgt
     5701 agtggcgaat tccagcgccg ggttcccgtc cgtattccct ttccacgcaa gcatcgccag
     5761 accgtcaggc agcggggcgc tcaacgcgtt cccgaattgt tcggtcgacc gcccacccgt
     5821 taccatgggt atagcagccg aaaagcccga agcccctccc gcgcccgcaa gtcccgtccc
     5881 cgccgtagtc cctcccgcgc ccgcaagtcc tcccgtcccc gccgtagccc ctcccgcgcc
     5941 cacaagtcct cccgtcgtcg tcccgatctt catcacaaca tcctcattca gtccctggat
     6001 atcgctgatg tctaaaaaca acctcgcctc cttccacaac atatcggagg ctgaaagacc
     6061 cagctcttcc agcggcaagc tcttgtaact gccgcggata tccatctgcg ttgtataaac
     6121 gatcacttca taaatacccc tgtaccgtcg ctgagggacg atactggcgt gaatgtccaa
     6181 tttgtcgggc aggaagtagg cccaccgttt gatgctctgc cggccggcat tgtcgctgac
     6241 cgtgtcataa taagggatgc cgatgacggg accggtcaca gtctgtggtc ccgcccaacg
     6301 cgatcctatc tcggcgactg cctgatgttg acggtcctgt ctttccgaca cgagattttg
     6361 aataaaggcg gtaggtatca gcaacagcag gatcaacgcg ccgacaagcg cacttttgaa
     6421 tagggtccgg aaattattcc agagtccggg agtcttgatt tccatgtttg ggttttatta
     6481 ttttaattca gcgcacggct tagtttcttc aacatgacgt agacaaaact gagtaatatc
     6541 gcatagcaaa ggatcatatc ccaggccggc cggttactca gcggatggat gaccatggtc
     6601 gcaatatcct ccatcagctg aaacggatcg gtgagaatgt cccagctatt ataccgtaaa
     6661 tatctgcccg tatatacccc caaagcgctg agcgcgatga cgggatagac aaagactgcg
     6721 gtaggataag gtaacggcac ttgcagccgc gggccgaaga tcgctttcga caatcgcttg
     6781 ttctccagca accgctccat ctgccgaagc gataacacac ccagcaggag tccgttccag
     6841 gcaaaagaga agatcatggc cagatcgaac cactccggca ccctcggatt cctgctgtcg
     6901 tgcaagtgga acatatcggt cagcatataa aaggcatttg ggaaaaagag cagccagatc
     6961 aggcagaccg ctacgataaa taacggcctg ctgctgatag gcctggataa ggatttggtt
     7021 aacgggtgat ccaaccgccg gtttggccag ctttccactc cttccaaccg ccggcttagc
     7081 cagctcgaca gcacataggg tatatatgct aaaaacagat tccagatcat aaatacgaac
     7141 aacaggctgt gcgcatggat catccgaagg ataaccagcc cgcagctgaa acacatcgaa
     7201 caggtgagca acctgtctgt ctcgctgcgc aaaaaccacc gccgccatgt gccacccgcc
     7261 tgaccaggat acgatatcat aataacttcg gtataaagat gaatgatccg gccgctatgg
     7321 ctacgatagc agccaccagc accgcccccg ctgcgagatc cttgatgatc tcgatctcgg
     7381 gatgtctttc cttcgtgatg tgatccgcca gtttttccac gcaggtattg agtatctccg
     7441 ccacccagac catcccgatc actgccgcga ggatagccgc ttccgtagcg gacacctcca
     7501 gtacccgggc gacgatgatg acgaggatcg ttgctacgag atggatacgc gcgttgtgct
     7561 ccctgttgac aaatcttcgg agcccggcaa tggcgtagtg gaaactcttt atcctggctc
     7621 gtactgaaaa tttttctgta tccatggttg agatgattag gcgattgcag gaaaaggcga
     7681 cagaggcaga cccggtttgc gatccccgtc cgacccccgc gaccccggtc ctcccggcgt
     7741 aaagtccccc gtcctcttca ccctcgaaag gatggcctcc agcaggggaa gcatcttatt
     7801 cagtccctgc gttttgatca gaaaattcca catgggctcg aacttcttcc atccccccgc
     7861 ataagagaaa tgtttgatct tatatttgag gctgtcatga ccgaggctgg cccgccagat
     7921 attgctccac gaatagaacg agcgataggc gtcatcataa ccctttttta gctcggcggg
     7981 cgtaagaccg acggtcttat acaccacctg acgcgtatca tactggtccc agttacggtg
     8041 taggatccgc ccctgcgctt ccatatcctt aaaaagacgg gtgccggggt atggggtcag
     8101 gatatgaaag gtacaggttg tcagcgaatt tctgaccgcc cattccaccg tccggtgaaa
     8161 aacaccgggg tcgtcggcat cgagaccgaa aacaaagctg ccgtttatca tgatgcccag
     8221 cgaatgaaga cgacggatcg cctcttcgta atcacgtccg atattctgtc tcttgttgct
     8281 ttgcacgaga ttgccctcgc tcaacgtttc aaagccgacg aatacactgc gcaagcccgc
     8341 ctcagcagcc cgctcgatca gatcgccgcg caggatggca tcgatggtcg acgcgccctg
     8401 gaagaccctg ttcatccctt tcattccgtc aaacaatgcg gttgcaaaac gctgctgtcc
     8461 taacaaatgg tcgtccagga aatacaaatg tcgcccggga agccgatcga tctctgccag
     8521 cgccgcatcg acccgctggg tataaaagga acgaccgcct tcaaaaaatg cgtccttata
     8581 gcagaaatca caatgatgcg ggcaaccccg cgaaaccacg atcgagttag gcaccaggta
     8641 acgttcgcgt ttgatcagat cgcggcgtat cggaggtatc tccgtgatat cccgctcggt
     8701 gtttctataa actctccccg gactcccttc ccgaaaatcc ttcaaaaacc tcggccacga
     8761 gtcttccccc ggtccgatga agatcgaatc cgcatgaacc tccgcctcat ccggcagact
     8821 cgtgacatga agcccgccca gacatacata cgcaccttta ctgcggtaat gatcggcgat
     8881 cgcataagcc ctgaacgcat ttgtaatgta gacctggatc accaccagat ccggctcgtc
     8941 gtccgaccgc agctcttcca catgctcgtc ctgcagatcg atgtcgtcat cgggcccgca
     9001 ataggccgcc agggtggcta accccagcgg cgggaacagc gagtatttga tagggcgcca
     9061 gaacgggctt ttcgcttcgg ttagcgcggg caagatcatt ttgattttca tagtcttaat
     9121 atctgagata ctcaatgaac aaataagcga caagccccac caaaaacaac gcaccctgca
     9181 aaacgaccgc gacattgagc cagctccggc ggaacccaag ttccacatcc ggccctatcc
     9241 gacccggcac gatccgtacc gtgaccaatg ccagcgcgtt gaacaccacg gccacgacca
     9301 gacagccgat catcagctgc gtctgctgag ccatatatcc atacatccat cgcgatccga
     9361 aacaaaccct tttgactgcc gtcaaaagga aaaaaattgc cggcagcatc agtgcgatcc
     9421 ctaccaggcc cgaggcgagg tgccgaagaa agataagacg tgtgatcgat tgattattca
     9481 tattgaaagt gctttgaaat acaaagtgtc aagcccaaaa aaatcgcggt ttcccgcgtc
     9541 accatcctca tcctcccgcc gccctcgtcc gccgctagcc acccccgcat caccgtcccc
     9601 ggcgccccca tcgccgccta ccccacacca ccccgaccac ttcctcccgc gctcccgcgt
     9661 ccccatcgcc cacctgcccc acaccatccc gacgtcccta ccccttactc acccccttga
     9721 tcatactctc caatgcctcc aggtgttcct taaaagcctt ctcccccgcc ttggtgatcg
     9781 aataggtcgt attcgtcttc ctaccgataa aacccttatg caccttaata aacccgttct
     9841 cttccagatt gatcaaatgc gaggcaagat ttccatcggt caattccagc aattgcttca
     9901 gatcgttgaa gctgacctcc tcattcacca ggaggatgct cataaccccc atccggatgc
     9961 ggctgtcgaa tatcttattg aggtttccga tcggattctt catgttctct cgtatttcca
    10021 ccacatgatc aggccataga tgatatgcag tatcccaaag cctgccgtcc aaaaatacaa
    10081 cccctcatgc ggaaaataca tgctgacgca tccgagcgcg atctccagca accccaggta
    10141 tcggatatcg gtcaacgtat acttgctggc attcaccagc gcgagaccgt aaaagatcaa
    10201 acaggccggg gcgacataga tccaatccgc attatacaac agccctatca caaaaagccc
    10261 ccccgcaacc agcgggatca tcatgttgat cgcaagtttc ttcgacgcat gatccagtat
    10321 cggcagccgg ctcctgcgcg ccttccgcca ggtgaacagg gtcgccgaga caagcgccgc
    10381 cgccagcacg actccggcca gcacgatcag cttccacttg aaatcgtatc gctcggtcgc
    10441 cacataacca tgcacagaca ggtatcgttc ataatagtcc tgtatccatc catgcgccac
    10501 ccatgccccg atgagcgcgc atacccccgc actgacgccg ctcagcccgc tcagcgagat
    10561 aaaccgcgaa ctgcgttcca tcatccgctt gatgtcctgt aaagcttcca gggattgctc
    10621 agccgggctt tgattgctca taaaagcact ttgaaataca aagtaatgga gaatttttta
    10681 ttcctgcaag tgtttttcca aaaaattatc ccaggcccga tcggttttaa tttcgaattt
    10741 ttgaattttt ttcttttatt gtgatataaa cccctctgac aatggaaaac gatcaacaag
    10801 aatccctcat ccagatgcac ctggattacg atggcggcaa catcctccgc gaaacagtcc
    10861 gatggtcccg ctttctctct atcgtaggta ttataggact ggcgctctgc ctgctgatat
    10921 ttgtgctggc cggctcagct attgtcgcag cattttccag gctgtcgccc ggcctcgaag
    10981 gactggccgg tcttggaggc gtgatcctcc taatcattat gatcgcattc tttgcgatca
    11041 gcggtttcct ggtatatatg cttttccgtt tttccaaaat gatacgcaga ggtatcgatc
    11101 atcaggacca ggccactttt tccgaaggca tgaaaagcct gaagacctac ttcatcatca
    11161 gcgggatcct cagcgccctc ctcctgctgt tcaacgcttt gtcattaatt caactttttt
    11221 aatcccaagc caccatggat agccaaccta catcccagac caccaattca ttggataatt
    11281 ttttcaatgt ttccttggat accgccacga ggtcccagat cagacaggcg gccgtatggg
    11341 ccagggtcac cgcgctgtgc gctttcggag catatgctat aacgcttatc gtggcttttt
    11401 ttggccgggt cgccggcctg gacgattccg caattggatt agaggtcaac gactctttcc
    11461 ggataaacag gatcatcggc gccctgatta ctgcggtgat aggcgtgatc atcaatctct
    11521 ttctttatcg cttcgcccaa tcgacgatcc gcgggatcga cgccatggac aacgtcctga
    11581 ccaacgatgg attcaaccaa ctacgcagat atttcaagat actcggtatt cttctgatcg
    11641 tcgtgttctc cctcgtcatc ctgggtatat tgattggcat cggcgtcgga cttagccgga
    11701 ccagcagtta ttgaccggca ttcggccatc ctgccgccat acatgatccc taccggcgcc
    11761 ctcgccccgg cggccccgca tccccgcatc cgctcacccc agcgtccccc acatcaccat
    11821 ccccgcatcc gcccaccccc gacagccccc caaatctgct cacccccagc gtcggaattc
    11881 ccgcatcccc cgccccagcc gcatccccac atcccggcgc ccctcgcccc gacagacctc
    11941 cgcatccacc caaccccggc ggcggaccct atctccccac cctcgccatc gccctcaccg
    12001 gaaaagtccc cacccccttg aacttcggcg gcaccgcact gaaaacaaac ccctcatccg
    12061 gcaacaatct caattcgcaa agatgctcca cgatcggtat ccccgccccc agcaaggtcg
    12121 tatgcaccgg ccggctccgc cccgccgtat catcgatatt atgcgagtcg atccccacca
    12181 gccgcgcgcc gctatccctc aaatactctg ctgcatccaa agtcagaaaa ggatgccccg
    12241 aaaaatacac cggagtattc caatgctcat cccaccccgt atggatcagc acggcccgac
    12301 cccggacctc aaaaccgcgc agatccgaaa ccctcaccgc cagcccccgt gaataaggta
    12361 tgcgaactac gatccccggc agttcggcta ccctctccaa tcccacttcc gacagatcat
    12421 gcccgtcccc aaatctatga aaaggaacat cgatatacgt ccccgtattc gccaccatct
    12481 cgatcttcgc gatctgaaat tccgtccccg gctcatacag cttccgcgat tcctcacggc
    12541 tgagataatc acatatcacc
//
LOCUS       DASYSV010000483         8766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1896205, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000483 DASYSV010000000
VERSION     DASYSV010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896205"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /gene="lepB"
                     /locus_tag="VGQ51_14875"
     CDS             <1..316
                     /gene="lepB"
                     /locus_tag="VGQ51_14875"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV9037916.1"
                     /translation="GGLVYDRKQFYVIPPYTDTLYISLEDKYVTENALPFERYMLPRG
                     LRDEDIYLQYKQNWNRDHFGPLKIPPGKFFVLGDNRGNSRDSRYLGLIDQSRYVGRVW
                     WK"
     gene            complement(317..1540)
                     /locus_tag="VGQ51_14880"
     CDS             complement(317..1540)
                     /locus_tag="VGQ51_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEV9037917.1"
                     /translation="MLRITGCLVLVFTMLTVMATPAPNFRNGWWRARLERKDGHDIVF
                     NFEVKDSGGRQVLYIRNAGERLLVDDIKREGDSVLIRMPFYESQLRARVTTEGNLEGV
                     WLRHLASEWQAMPFKAVYGEGYRFAGSEPGAGVSVSGRWAAVFRAPGDSTFRVGEFEQ
                     RGSRVTGTFLDAGGDLRYLEGVVAGDSMKISCFDGTHAYFFTARIDGNTMTGMQYSGP
                     AASATWTAHKDASAKLENQFAMTKWNKDKPFTFTFRDLDGHEVSLSDPRFKGKVVLVQ
                     IMGSWCPNCMDETRFLSGFYDQYQSKGVEVVALAYERSTDFARSSSSVRTFQQRFHVR
                     YPMLITGVTVGDPQRTEKTLPQLDAIADFPTTIFVGKDGKIAMIHTGFSGPGTGAHYE
                     DQKKEFYTIVDQLLK"
     gene            complement(1669..2610)
                     /locus_tag="VGQ51_14885"
     CDS             complement(1669..2610)
                     /locus_tag="VGQ51_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV9037918.1"
                     /translation="MVKEKVLVIGASGQIGVELTLALRRIYGNSNVVASDLREENELL
                     RGSGPYVSLDVMNKEMLHVQVIRQNITQIYLLAAILSATGEKNPNLAWHLNMQGLLNV
                     LDIAREEKLHKVYWPSSIAVFGPTSPRQNCPQRTIIEPSTVYGISKYAGEFWCNYFFE
                     RYGVDVRSIRYPGLISWKSAPGGGTTDYAVEIFHEALEQGHYDCFLKEDTYLPMMYMP
                     DAIRATIELMEAPADRLSVRTSYNVSALSFSPHEISAEIKKKIPGFTIRYAPDYRQHI
                     ADSWPQSIDDSVARAEWNWKPEYDLARMTADMLENLR"
     gene            2662..3492
                     /gene="kdsA"
                     /locus_tag="VGQ51_14890"
     CDS             2662..3492
                     /gene="kdsA"
                     /locus_tag="VGQ51_14890"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEV9037919.1"
                     /translation="MEFLHQLFKHQSYDKNNFFLIAGPCVVESEELIMEVADKVSGIC
                     RNLGIPYVFKASYRKANRTSAGSFTGLGDDTGLELIGKVGKTYRLPTTSDIHAHDEAG
                     PAAKYLDILQIPAFLCRQTDLLVAAADTGKIVNVKKGQFLSGPAMKFAVEKIRKAGNE
                     KIILTERGTTFGYQDLVVDYRNIPWMKEHGTPVVMDCTHSLQQPNQASGITGGNPQLI
                     GTIAKAAIAAGADGIFIETHPNPAIAMSDGANMLRLDLLQGLLEHLVRLRKVVNGFQG
                     "
     gene            complement(3671..4663)
                     /locus_tag="VGQ51_14895"
     CDS             complement(3671..4663)
                     /locus_tag="VGQ51_14895"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9037920.1"
                     /translation="MFQLDIDHTNFEELTNDLAQRLNARVEKDRIKLPEAYASGVFSF
                     VILPNGMHVNIADFIFNQDCLINRQKTHEQYYILRFHDLTIPDMLEIRIGNEWLKETR
                     TSRQIAYLSNTLTEWAYFAAKGTIYKGINILFNSTGLAEQLGVAQLEDVLSTYLSLEI
                     DNINREPLDIEYKRLMQEIMDVEETNPLRLAVIQNRVMLLIERFFLRLYERRKNHYFH
                     VPMSKADIDRVMQVEATLTKDIFEPAPTINQLARMVSISESKLKKDFKLIYGLPVFEY
                     FQKVRMQAAKDKLLGGNYSVKEVAMELGYSNLSNFTIAFKKEFGLLPSKLLSSN"
     gene            complement(4759..4980)
                     /locus_tag="VGQ51_14900"
     CDS             complement(4759..4980)
                     /locus_tag="VGQ51_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HEV9037921.1"
                     /translation="MKRMKFFIEWQVFGVCTAIGDRMGIATSRIRTWFMYISFLTMGS
                     PLILYFILAFWMNIRRYILSARRNPIRYL"
     gene            5249..7471
                     /locus_tag="VGQ51_14905"
     CDS             5249..7471
                     /locus_tag="VGQ51_14905"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEV9037922.1"
                     /translation="MPSKVDAARKLVGKPLTLAEKILYSHLFEKPAQAYRRGKDYVDF
                     APDRVAMQDATAQMALLQFMTCGRDKVAVPSTVHCDHLIQAKTGAVADLATALDVNKE
                     VYDFLASISNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTPNAGGLGMVAIG
                     VGGADAVDVMAGLAWELKMPKLIGVRLTGKMSGWTSAKDIILKVAGILTVKGGTGAIV
                     EYFGEGADSLSATGKGTICNMGAEIGATCSLFAYDTKMAAYLKATGREEVASLADSIR
                     DYLRPDKEVYADPKKYYDQLIEIDLNELEPHVNGPFTPDLAWPISKFAEAVKANNWPE
                     RLEVALIGSCTNSSYEDISRSASLAQQAIDKNLKTKAEFTITPGSEQVRFTIEKDGFL
                     NTFDRIGGVVLANACGPCIGQWARHIDDPNRKNSIITSFNRNFAKRNDGLASTHAFVA
                     SPELVTAFAIAGHLSFNPLKDKLTNEKGEQVMLDEPKGVELPPKGFSVDDAGYQAPAK
                     DGKNVQVVVKSDSQRLQLLEPFAAWEGTDLKGLKLLIKAKGKCTTDHISMAGPWLKFR
                     GHLDNISNNMLIGAINFFNDKTDDVKNELSGQYGPVPATARAYKAARIGSVVVGDENY
                     GEGSSREHAAMEPRHLGVRAIVVRSFARIHETNLKKQGMLALTFASKEDYDKVQEDDS
                     IDIVGLTSFAPGKSLTMVLNHKDGSKDEVVLNHTYNEQQIGWFKAGGALNVIRKQFAK
                     "
     gene            complement(7480..8415)
                     /locus_tag="VGQ51_14910"
     CDS             complement(7480..8415)
                     /locus_tag="VGQ51_14910"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV9037923.1"
                     /translation="MPIRRRDFLSASLAALTGSLMARTPAPGTEGLALGKAAPEAFGD
                     HPGRTVTVQQVIDALQADVPGAPFADTVDTIKCGDPSTPVRGIVTTMFPTDEIIQKAI
                     DLGANFIIPHEPTFFSGGDNVQWLADDPVYKYKRGLLEKNGIVIWRFHDGLHAHQPDG
                     VRMGVIQAVGWEPYFSAGDPSLINLPSAASLADLIALFKKSLGIEHVKYIGDLAQPCS
                     RIVVCPGAYNGRTQMNLIQQYRPELFICGEIREWETNQYVLDARYSGQKISLLILGHS
                     LSEEPGLEWLRTQLKIKFPEVPVTHLPSGDPFQWE"
     gene            8486..>8766
                     /locus_tag="VGQ51_14915"
     CDS             8486..>8766
                     /locus_tag="VGQ51_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEV9037924.1"
                     /translation="MTRRLVQVLILTVLVYSGNAQTASNARRLAIIGYYAGRNTAIDS
                     LPMQRLTHVIFSFCHLRGNRLFVNNAGDSDRIRHLVSLKVQNPSLKVILS"
BASE COUNT         2169 a         2390 c         2245 g         1962 t
ORIGIN      
        1 cggcggcctg gtatatgatc gcaaacagtt ctacgtcatt ccgccgtata ccgatacgct
       61 ttacatctcg ctggaggaca aatacgtaac cgaaaacgcg ctgcctttcg agcgctatat
      121 gctgccccgt gggctgcgcg acgaagatat ttatctgcaa tataaacaga actggaaccg
      181 ggatcatttc gggccgttga agatccctcc cggaaaattc ttcgtattgg gtgataaccg
      241 cgggaacagc cgcgattcgc gctacctcgg cctcatcgat caatccaggt atgtaggcag
      301 ggtctggtgg aagtgatcac ttcagcagct gatccacgat ggtataaaac tctttctttt
      361 gatcctcata atgggcgccg gtaccgggcc cgctgaatcc ggtatgtatc atggcaattt
      421 tgccgtcctt cccgacaaag atggtggtgg ggaaatcggc gatagcatcc agttgcggca
      481 gtgtcttttc agtccgctgc ggatcgccta ccgtcactcc cgtgatcagc atgggatacc
      541 ggacatggaa ccgttgctgg aatgtacgaa cgctactgct actacgggcg aaatcggtgc
      601 tgcgttcata ggccagtgcg acgacctcca cacctttact ttgatattga tcataaaacc
      661 cgctgaggaa gcgtgtttcg tccatgcagt tagggcacca ggagcccatg atctgaacca
      721 gcacgacctt gcccttgaaa cgcggatcgg acaaggaaac ctcatgtccg tccagatccc
      781 ggaaagtaaa agtgaacggt ttatccttgt tccatttggt catggcgaac tggttttcca
      841 gtttcgcact tgcgtcctta tgcgccgtcc aggtagcgga ggcggcagga cctgaatatt
      901 gcattcccgt catcgtgttg ccgtcgatcc gggccgtaaa aaaataagca tgtgtcccgt
      961 cgaaacagga gatcttcatc gagtcgcccg cgaccactcc ctcgagataa cgaaggtcgc
     1021 cgcctgcatc cagaaaagta ccggtgaccc tcgaccctct ctgctcgaat tcccctacgc
     1081 ggaaagtcga gtcgccagga gcgcgaaaca ctgccgccca ccgtcccgat acactgacgc
     1141 ccgcacccgg ctcactgccc gcaaaacgat acccctcgcc atacacggcc ttgaagggca
     1201 tcgcctgcca ctcgctggca agatgcctta gccagacccc ctccaggttc ccttcggtcg
     1261 ttacacgcgc ccgaagctgc gattcataaa aaggcatccg gatcagcacg gaatcacctt
     1321 cgcgtttgat gtcgtcgacc agcagccgct cgcccgcatt gcggatatac agcacctgtc
     1381 tgccgccgct gtccttcacc tcgaaattaa aaacgatatc atgaccatcc ttccgttcca
     1441 gcctggcacg ccaccagcca ttacggaaat tgggagcagg tgtcgccatt acggtaagca
     1501 tggtaaaaac aaggaccaga cagccggtaa tacggagcat agagagtcgt gttgaacctg
     1561 caagataaga aaaattaagt ctaccggatc agtagagtgc atgggagcac cggcaatcgc
     1621 atatctcccc gcagtctccc ggcaatcagc catgggccgc cacccctcct accgcaaatt
     1681 ctccagcata tcggcggtca tccttgccag gtcatattcc ggcttccagt tccattcggc
     1741 ccttgcgacc gaatcatcga tactttgagg ccagctgtcg gctatatgct gccggtaatc
     1801 aggagcatac cggatcgtga acccgggaat cttcttcttt atctccgcag agatctcatg
     1861 gggagaaaat gacaatgccg atacattata ggaggtacgg acggacaacc ggtcggcagg
     1921 cgcctccatc agttcgatcg tcgcgcgaat ggcatcgggc atatacatca tgggcaggta
     1981 agtatcttcc ttcagaaagc aatcgtaatg accctgctcc aatgcctcat ggaatatctc
     2041 gacggcatag tcggtggttc caccgccggg cgccgatttc cagctgatga gacccgggta
     2101 tcggatgcta cgcacgtcta ccccataccg ttcgaaaaag taattgcacc agaattcacc
     2161 ggcatatttg ctgatgccat agaccgtact tggctcgatg atggtccgct gcggacaatt
     2221 ctgacggggg gaagtgggcc cgaatacggc aatgctgctg ggccagtaca ccttatgcaa
     2281 tttttcctcc ctcgcaatat cgagcacatt cagcaaaccc tgcatattca ggtgccaggc
     2341 cagattgggg ttcttctcgc cggtggccga aaggatggcg gccagcaaat agatctgcgt
     2401 gatgttctgc cggatcacct gtacatgcag catctctttg ttcatgacat ccagcgacac
     2461 ataaggcccc gagcctctca gcaattcgtt ctcttcccga aggtcggaag ctaccacgtt
     2521 ggaatttcca tagatgcggc gaagagccag cgtcagttct acgccgatct gcccgctcgc
     2581 gccgatgacc agtacttttt ctttgaccat gtaatgggga tttggggaac aaacgtaggc
     2641 aaaaaaacgt agtttcgcgg catggaattc ctgcatcaac tgtttaagca ccaatcctac
     2701 gacaaaaaca atttcttcct catcgcaggc ccctgcgtcg tggagagcga agaattgatc
     2761 atggaggtcg ccgataaggt atcggggatc tgtcgcaatc tgggcatccc ctacgttttc
     2821 aaggcctctt atcgcaaggc taaccgcacc agcgccggca gcttcaccgg tctgggggac
     2881 gacacgggtc tcgagctcat cgggaaggtg ggaaaaactt atcgcctgcc cacaacttcg
     2941 gatatccatg cccatgacga ggccgggccg gcggcaaagt atttagacat tttgcagata
     3001 cctgcctttc tgtgccggca gaccgatttg ctggttgccg ccgcggacac cggtaagatc
     3061 gtcaatgtga aaaagggtca gttcctcagc gggccggcca tgaaattcgc ggtggaaaag
     3121 atcagaaagg cggggaatga aaagatcata cttaccgaaa gaggaacgac attcggctac
     3181 caggaccttg tcgtcgatta tcgcaatatt ccctggatga aggagcatgg gactcccgta
     3241 gtaatggact gcacgcattc actgcagcag cccaaccagg ccagcggaat caccggcggc
     3301 aatccacaac taatcggtac tatcgcaaaa gctgccattg ccgccggcgc cgacggtatc
     3361 ttcatagaga cacatcccaa tcctgccata gccatgagcg acggcgcgaa tatgctgcga
     3421 ttggatcttc tgcaagggtt attggagcac ctggtgagat tacgtaaggt cgtaaatgga
     3481 tttcaagggt gaggactggt ctttctggat gtactaaccg gccttgccga tcgatgagga
     3541 attaaaaacg ggacctttaa ctattctgac acaaagttac gatcatccgc acgcaatcat
     3601 cccctaaaaa agttaaatag atcgctgtaa aatccacggt tcggacattg cgtccgccga
     3661 aaatcctcaa ttaattggaa gacaatagct tacttggcaa tagtccgaat tcttttttaa
     3721 aggcaatcgt aaaattgctc agattggaat aacccagctc catggcgacc tccttgaccg
     3781 aatagttacc tccaaggagc ttgtccttgg ccgcctgcat cctgaccttt tgaaaatatt
     3841 caaatacggg cagaccatag atgagcttga agtccttttt aagtttggac tcgctgatgg
     3901 ataccatccg ggccagttgg ttgatggtcg gagcaggctc gaaaatatct ttcgtcaatg
     3961 tggcctcgac ctgcattacc cgatcgatat cggctttgga catgggaacg tggaaataat
     4021 ggttcttccg gcgctcgtag aggcgaagaa aaaaccgctc gatcagcaac atcaccctgt
     4081 tctgtatcac cgccagccgg agagggttgg tttcttccac atccatgatc tcctgcatca
     4141 atcgcttata ttcgatgtcg agcggctcac ggttgatatt atcgatctcc aatgaaagat
     4201 aggtcgaaag gacatcctcc aattgtgcca cacccagttg ttctgcgagc ccggtggaat
     4261 tgaacaatat attaatgcct ttatagatcg ttccttttgc cgcaaaatag gcccattccg
     4321 tgagggtatt gctgagatac gcgatttgcc tggaagtccg ggtttctttc agccactcgt
     4381 tcccgatccg gatctcaagc atatcgggga tcgtcaggtc gtggaaccga aggatataat
     4441 actgttcgtg agtcttttgc cggttgatca gacagtcctg gttaaagatg aaatcggcga
     4501 tattgacatg catgccgttt ggcaatatca caaaactaaa gacgcccgag gcataagcct
     4561 ccggcaattt gatccgatcc ttctccaccc gcgcattcaa ccgctgcgcc agatcgttgg
     4621 tcaactcttc gaaattcgta tggtcgatat cgagttgaaa caaagagatg ggtttgcaga
     4681 aagatacgaa gaaatagtaa cccggccctt cgggtaaagg aaaggatttg cgaaaatacc
     4741 catcggcctt cggtggcctt acaaatacct gatcggattg cgtcttgcag aaaggatata
     4801 acgccggata ttcatccaaa aagccaggat gaaataaagg atcagaggcg aacccatggt
     4861 gagaaaagag atgtacatga accaggtccg gatacgggaa gtggctatgc ccattctatc
     4921 accgatggcc gtacatactc cgaatacctg ccactcgatg aaaaatttca ttcgcttcat
     4981 gaggctaaat tataaaaaaa tcgtccacta ccaaaccacg acccccggtt tttatgtgcc
     5041 gcaaatgccc ttcaacgaaa aatagggcat ttgcggtaat ccactaatga ttgtccttcg
     5101 gacggaccga cttcgtcggt ttttgtagcc cccgaaagca atttttaggt aattttgcgg
     5161 tcgcaacagc atcttctgat ttgttgacat attaaataaa ttcatcatgg tcttcgatct
     5221 ggatctaatc aaaaagttat acagggaaat gccgtccaaa gtggacgcag cccgtaaact
     5281 ggtaggcaag cctttgactt tagcagaaaa aatcctctat tcgcatcttt tcgaaaagcc
     5341 cgctcaggca tatcggcgtg gtaaagacta tgtggatttt gcgccggacc gcgtcgcgat
     5401 gcaggacgcc acggctcaga tggccttact tcagtttatg acctgcggcc gcgacaaagt
     5461 tgcagttccc tcgacggtgc attgcgatca cctcattcag gccaagactg gcgcagtcgc
     5521 cgacctggcc acggcactcg atgttaacaa agaagtatac gatttcctcg catccatctc
     5581 taataaatat ggtatcggat tctggaaacc gggcgcgggc atcatccacc aggtggtgct
     5641 cgaaaattat gccttccccg gcggcatgat gatcggtacg gattctcaca cgccgaacgc
     5701 aggcggtttg ggcatggtag ccatcggcgt tggtggtgcg gacgccgtgg acgtcatggc
     5761 gggtcttgcc tgggagctca agatgcccaa actcatcggt gtcaggctca cgggtaagat
     5821 gagtggctgg acaagcgcta aagatatcat cctgaaagtg gccggcattc tgacggtaaa
     5881 aggtggtaca ggcgctatcg tggaatattt cggtgaaggc gcagacagcc tgagcgccac
     5941 gggaaaaggg actatctgca atatgggtgc agagatcggg gccacctgct cgctgttcgc
     6001 ctatgatacc aaaatggcgg cttatctgaa ggcaaccggc cgcgaagaag tagcatctct
     6061 ggccgacagc atccgcgact atcttcgccc cgataaggag gtctacgccg atcccaaaaa
     6121 atattatgat cagctgatcg agatcgatct gaatgaactg gaaccccatg tcaatggtcc
     6181 attcactccc gacctcgcct ggcccatcag caagttcgcg gaggcggtga aggcaaacaa
     6241 ctggcctgag cggctcgagg ttgctctcat tggcagctgt accaactcct cttatgagga
     6301 catcagccgt tcggcatcgc tcgcacaaca ggccatcgat aaaaatctca agaccaaggc
     6361 cgagttcacc attacgccag gttcggaaca ggtgcgtttt accatcgaaa aagacggttt
     6421 cctgaacact ttcgaccgca tcggcggtgt ggtcctggcc aacgcctgtg gtccctgtat
     6481 agggcaatgg gcacgccata tcgacgatcc caacagaaag aattccatca tcacttcctt
     6541 caaccgcaat tttgccaagc gtaacgacgg actggccagc acgcacgctt ttgtcgcgag
     6601 ccccgagctc gtcactgctt tcgccatcgc cgggcatctc tctttcaatc cgctgaaaga
     6661 taaactgacc aacgaaaaag gcgaacaagt aatgctggac gagccgaaag gcgtcgaact
     6721 gcctcccaaa ggattttcgg tcgacgacgc cggataccag gcgccggcaa aagacggaaa
     6781 gaatgtacag gtggtcgtta aatccgatag ccagcggcta cagttgctgg agccgtttgc
     6841 ggcatgggaa ggcaccgatc tgaaaggact gaagctgctg atcaaggcca aaggcaaatg
     6901 cacgaccgac catatctcca tggcgggtcc ctggctgaaa ttccgcggcc atctcgacaa
     6961 tatctccaac aatatgctca tcggcgccat caatttcttc aatgacaaga ccgacgacgt
     7021 aaagaatgag ctctccggtc agtacggtcc ggtgcctgct accgccaggg cttataaggc
     7081 ggcccgtatc ggttcggtgg tcgtgggaga cgagaactat ggcgaaggca gcagccgcga
     7141 acatgcggcc atggagcccc gacatctggg cgtccgtgcc atcgtcgtcc gtagctttgc
     7201 gcgcatccac gagaccaatc tgaaaaaaca gggcatgctc gcgctgacgt ttgcaagcaa
     7261 ggaagattac gacaaagtac aggaagacga tagcatcgat atcgtcggtc tgacttcctt
     7321 tgcgccgggc aagtcgctga ccatggtgtt gaatcataag gatggcagca aggatgaggt
     7381 cgtgcttaac catacatata atgaacagca gatcggctgg ttcaaggcag gtggagcgct
     7441 gaatgtgatc aggaaacagt ttgcgaaata gactttcgct cactcccatt gaaaaggatc
     7501 ccccgaggga aggtgtgtca ccggtacctc ggggaatttt attttcagct gggtccgcag
     7561 ccactccaga cccggctcct cgctcagcga atgcccgagg atcagcaacg atattttctg
     7621 acccgaataa cgcgcgtcga ggacatattg attggtctcc cattcgcgga tctctccaca
     7681 gatgaacagc tccggcctat actgctggat cagattcatc tgggtccggc cgttgtaagc
     7741 gcccgggcaa accacgatcc tgctgcacgg ttgagctaga tctccgatgt atttgacatg
     7801 ttcgatacca agactcttct tgaacagcgc gatcaggtcg gccagcgatg cagccgaagg
     7861 caggttgatc agcgacgggt cgccggcgct aaagtagggt tcccatccaa cggcctgtat
     7921 gacccccatc cgcacgccgt ccggctgatg agcgtggagt ccgtcgtgga aacgccagat
     7981 cacgatcccg ttcttttcca gcagaccacg tttgtattta tacaccggat catccgccag
     8041 ccattgcaca ttgtccccgc cactgaaaaa agtaggctca tgcgggatga tgaaattggc
     8101 gccaagatcg atggcttttt gaatgatctc atcggtaggg aacatagtgg tgacgatgcc
     8161 cctgacagga gtggaaggat cgccgcactt gatcgtatcc accgtatcgg cgaagggcgc
     8221 gccgggaacg tcggcctgca gcgcgtcgat cacctgctgt acggtaacgg ttcgccccgg
     8281 atgatcccca aacgcttcgg gagccgcttt ccccaaagcc aggccctccg ttccgggtgc
     8341 gggagttctg gccatcaacg acccggtcag agccgccagg cttgccgaca aaaaatctct
     8401 tctgcgaata ggcatagtaa tgttttaata gctaaaacgt atatatgagc cggataaccc
     8461 ctaccttgaa cattaaatca tccttatgac ccgtcgtctg gtccaggtgc tgatcctgac
     8521 ggtcctcgtc tatagcggta acgcccaaac ggcctccaat gcccgacggc tggccatcat
     8581 cgggtactac gcaggacgga acacggccat cgacagtctt ccgatgcaaa gactgaccca
     8641 tgtcatcttc agtttttgcc acctccgggg taacaggctg ttcgttaaca atgcaggtga
     8701 cagcgaccga atccggcatc ttgtctcgtt gaaagtacaa aatccctccc tgaaagtgat
     8761 cctttc
//
LOCUS       DASYSV010000484         3866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2531575, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000484 DASYSV010000000
VERSION     DASYSV010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531575"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1162
                     /locus_tag="VGQ51_14920"
     CDS             59..1162
                     /locus_tag="VGQ51_14920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV9037925.1"
                     /translation="MPLPEIIIDTIRRQGPLSFHDFMEMALYFPGEGYYTSPGDCIGR
                     SGDFYTSAYLTHLFGDMIASQLEEMWLALGSRPFTVVECGPGTGLLCVDILRRLRYNK
                     TFFEHLQYILVEKSEWMREKERKLLETEGLLDKVRWANSLDQLSPVTGCILSNELIDN
                     FAVHQVEMQDQLMEVFVGYDDGFTEILRPAGADLKAYLHRLEVTLPAGHRAEINLEAT
                     QWIRQASAALEKGFIITIDYGNSSSGLYHNKTGTLICYHHHSMNHSPYTFIGEQDITS
                     HVNFSALDHWGRQEGLEHCGYTSQARFLQGLGLNRRLQEIEKQGDAAGDAQRARQVYT
                     LLVEMGNSFKVLIQRKGIGRSFLSGLQFAQYLA"
     gene            1242..3062
                     /locus_tag="VGQ51_14925"
     CDS             1242..3062
                     /locus_tag="VGQ51_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV9037926.1"
                     /translation="MSYQPIENYGIIGDLHTIALVGLNGSIDFFCYPRYDAPSIFARL
                     LDDKKGGYYCLSPEFSEMKTKQLYMPDTNVLLTRFLSPDGIGEITDFMPVGHTEEKKL
                     VRRVTTIRGESKYRLECRPRFNYGRDSHTAEQIGPMEILFRSDGLSIRLKAGVPLRIE
                     NGDVLSEFSLKAGSTADFVLEIAENKTSGADDLGEMVTQTLFRTVNYWKNWIGRSTYN
                     GRWVDVVNRSCLVLKLLASSRYGSIIAAPTFSLPESIGGQRNWDYRYTWIRDASFTVY
                     TLIRLGYSKEAGHFMEWVEKMFRDMKSDERLGIMYSIDGKRQLKEYQLDNFEGYKCSA
                     PVRVGNDAYGQLQLDIYGELMDSVYLYNKYGEPISYDFWKDLSAQMDWLSHNWKQPDD
                     GIWEVRGGKQFFLNSRLGCWVALDRAIRIAETRSFPIGEKWKEQRDAIYHSIYKDFWD
                     DKKQSFMQYPGAQTVDASSLLMVLFRFISPKDPRWLSTLKMIDEQLVTDALVYRYLPE
                     VAAPDGIVGREGAFSMCSFWYVECLSRSGQLDKARFIFEKMLGYANHLGLYAEQLGFE
                     GEHLGNFPQAFTHLGLISAAYNLDQQYNDNQNKNINLDEY"
     gene            3119..>3866
                     /locus_tag="VGQ51_14930"
     CDS             3119..>3866
                     /locus_tag="VGQ51_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV9037927.1"
                     /translation="MRFRRWLKILGPGLVTGASDDDPSGIVTYTQAGAQFGMATLWTA
                     LITFPLMASMQGMAARIGLVTNQGLTGTLRTHYPKWVLYLMILFCFPATILNIGADIQ
                     SMGAVAHLLVPGIPAFSFSIVFTAVLLFMIIRYPYQKMAGLLKWLCLALMTYLIVPFL
                     VHPHWGTVLHHTFIPTVRLDKNFLSILVAILGTTISPYLFFWQATMEVEDQQHNGRKG
                     GQAAMEAEGQHPSRIRRVYVDKRVLEDMRSD"
BASE COUNT          889 a         1054 c         1055 g          868 t
ORIGIN      
        1 tggcaatagt ggttgtaggc ataacaattg attttatccc taaaaaccct caattgtcat
       61 gcctcttccg gagattatta tcgacaccat ccgtcgacag ggacccctat cgtttcacga
      121 ctttatggaa atggcgctat atttccccgg agaggggtat tatacctccc ccggcgactg
      181 tatcggccga tccggcgact tttacaccag cgcctatctc acacaccttt ttggggatat
      241 gatcgctagc caattggaag agatgtggct ggcgctgggc tcccggcctt tcaccgtggt
      301 ggaatgcgga cccggcaccg gattgctttg cgtggatatc ctccgcaggt tgcggtataa
      361 taagaccttt ttcgaacatc tgcaatacat cctggtcgag aaaagcgaat ggatgcggga
      421 aaaggaaagg aagctgttgg aaacggaagg cctcctggac aaggtccgat gggcgaacag
      481 tctcgatcag ttgtctcccg tgacggggtg tatcctgtcc aatgagctga tcgacaattt
      541 tgccgtgcac caggtggaaa tgcaagatca gctgatggag gtgttcgtag ggtatgacga
      601 tggattcacc gagatcctgc gccctgcagg ggcggatctg aaagcctatc tgcatcggct
      661 ggaagtcacc ttgcccgcgg gacatcgcgc ggagatcaat ctcgaggcga ctcagtggat
      721 ccggcaggcc tccgccgcat tggagaaagg gttcatcatt accatcgatt acgggaattc
      781 ctcatccggg ttgtatcata acaagacggg gacgctgatc tgttatcatc accactctat
      841 gaatcatagc ccttatacct ttatcggcga acaggatatt acaagccatg tcaatttttc
      901 ggcgctggac cactggggcc ggcaggaagg tctcgaacat tgcggctata cgagccaggc
      961 acgtttcctt cagggtcttg ggttgaaccg gcggctgcag gagatcgaaa agcaggggga
     1021 tgccgcggga gacgcgcagc gggcgcgcca ggtgtatacc cttctggtag agatgggcaa
     1081 tagcttcaaa gtacttatcc agcgtaaagg catcgggcgt agctttcttt cggggttgca
     1141 gttcgcccaa tatctggcgt aaggaaggcc ggggccgcag gcccgtcgtc cgccagcccg
     1201 caggcccggt ttttgtaaca cactcataaa catcatcgcc agtgagctat caacccattg
     1261 agaattacgg aatcatcggc gacctccata cgatcgcact cgtcggtttg aacggctcca
     1321 ttgatttttt ctgttatcca cggtatgatg ctccaagtat attcgcgcgc ttgctcgacg
     1381 ataaaaaagg aggatactat tgtctgagtc ccgaattcag cgagatgaag accaaacagc
     1441 tatacatgcc cgataccaat gtgctgctga cccgtttcct ctcgccggat gggatagggg
     1501 agatcaccga tttcatgccg gtaggccata ccgaggagaa aaagctggtc cgccgggtga
     1561 cgacgatccg gggcgaatcg aaataccgtc tggagtgtcg tcctcgtttc aattatggca
     1621 gggatagcca caccgccgag cagattggac ccatggaaat attgttccgc tccgacggtt
     1681 tgtccatccg cctaaaggcg ggtgttccgc tgcggataga gaacggagat gtcctctccg
     1741 aattctccct gaaagccgga agcacggcag acttcgtcct cgagatcgcc gagaacaaga
     1801 cctccggcgc ggacgatctc ggcgagatgg tcacacagac gcttttccgt accgtcaatt
     1861 actggaagaa ttggatcggc cgcagtacct ataacggtcg ctgggtagac gtcgtcaacc
     1921 gcagctgcct ggtgctgaag ctgctggcca gcagccgtta cggatcgatc atcgccgccc
     1981 ccacgttcag cctgccggag tccatcggcg gacagcgcaa ctgggattac cggtatacct
     2041 ggatccgcga cgcgtccttc acggtctata cgctgatcag actgggatat tcgaaggagg
     2101 ccggccattt catggaatgg gtagagaaga tgttccgtga catgaagagc gacgagcgtc
     2161 tcggcatcat gtattccatc gacggcaaac gacagctgaa agaatatcag ctggacaatt
     2221 tcgaaggata taaatgcagc gcccccgtcc gtgtcggcaa cgacgcctat gggcaattgc
     2281 agctcgacat ttatggcgag ctgatggatt cggtatattt gtacaacaag tacggagagc
     2341 cgatctccta tgatttctgg aaggaccttt ccgcacagat ggactggctc agccataact
     2401 ggaaacagcc cgatgacgga atctgggaag tcagaggcgg caaacaattc tttctcaact
     2461 cgcgtctggg ttgttgggtg gcgctcgaca gggcgatcag gatagcggag acacgttcct
     2521 ttcccatcgg agagaagtgg aaggaacaac gcgatgcgat atatcacagt atctacaagg
     2581 atttctggga tgacaaaaaa cagtccttca tgcagtaccc cggggcccag accgtcgacg
     2641 catccagtct gctgatggtg ctcttccggt tcatcagtcc aaaggacccc cgctggctgt
     2701 cgacgctgaa aatgatcgac gaacagctgg tcaccgacgc gctggtttat cggtatctac
     2761 cggaagtggc cgccccggac ggtatcgtcg gaagggaagg ggccttctcg atgtgcagct
     2821 tctggtatgt cgaatgtctc tcgcgttcag gacagctgga caaagcccgt ttcattttcg
     2881 agaagatgct cgggtatgcc aaccacctcg gtctgtatgc cgagcagctc ggtttcgaag
     2941 gcgaacacct ggggaatttc ccgcaggcct ttacccatct gggtctgatc agtgcggcat
     3001 acaacctgga tcagcaatac aatgacaatc aaaacaagaa tatcaatctc gacgaatact
     3061 aatgccccaa cccaccaacc gccctccatc aaaacgccgc acacggaatc ccctcctttt
     3121 gcgttttcgc cgatggctaa agatcctcgg ccccggtctc gtcaccggcg ccagtgatga
     3181 cgacccgtcg ggtatcgtaa cctatacaca ggccggagca caattcggaa tggcgacact
     3241 gtggaccgcg ctcatcacct tcccgctgat ggcatcgatg cagggcatgg ctgccaggat
     3301 cggcctcgtc accaaccagg gtctcaccgg caccctccgg acgcattatc cgaagtgggt
     3361 gctttacctc atgatccttt tttgttttcc cgcaacgatc ctcaatatcg gcgccgatat
     3421 ccaaagcatg ggagccgtag cccatctgct cgtgcccggc attcccgctt tttcattcag
     3481 cattgttttt accgccgtgc tgctgttcat gatcatccgg tatccttatc agaaaatggc
     3541 cggattactg aaatggcttt gtctcgccct gatgacctat ctcatcgtcc ctttcctggt
     3601 gcatccccat tggggaaccg tcctccatca tacatttatc cccaccgtca gactcgacaa
     3661 gaattttttg agcatcctag tcgccatcct ggggacgacc atctcgccct atcttttttt
     3721 ctggcaggct accatggaag tggaggatca gcaacacaat gggaggaagg gagggcaggc
     3781 ggccatggag gcggagggcc agcatcccag ccgcatcaga cgggtctatg tagacaagag
     3841 ggtgctggag gatatgcgca gcgatg
//
LOCUS       DASYSV010000485         5680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1124998, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000485 DASYSV010000000
VERSION     DASYSV010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124998"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VGQ51_14935"
     CDS             <1..475
                     /locus_tag="VGQ51_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9037928.1"
                     /translation="LMTYMLYNTENETIFLEDEIGYLRAYIDLQMLRFGGDVTVSLNV
                     PENPGGSRIEPMLLIPLVENAFKHGIGLVNDPLILIDIETPDDKRLVMTVKNKYSPLV
                     RERENKPAGIGLSNLTRRLEMMYPGCYELERTDKLRLDVGESEHWFIIQLNIPLQ"
     gene            472..1182
                     /locus_tag="VGQ51_14940"
     CDS             472..1182
                     /locus_tag="VGQ51_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9037929.1"
                     /translation="MINCLAVDDETPALDILEDNIRQVPFLRLVGKCKNAFAAMEVLQ
                     QQPVDLLFLDIEMPGISGLSLLKSLPGKPIVIFITAYRNYAVEGFDLDVLDYLVKPVA
                     FDRFVRAANKALEYQGFKLKGAGSPEAAPDYLFIHTEYQLTKIFLNEIVYIEALRNYI
                     RIHLTAAAKPILSKLSLKAIEERLPPARFTRVHKSFIVMLDKIDFVRNDIIRIGQRDI
                     PLSRGCRDEFFRRINEIR"
     gene            1228..2388
                     /locus_tag="VGQ51_14945"
     CDS             1228..2388
                     /locus_tag="VGQ51_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEV9037930.1"
                     /translation="MNQRYYSLDVFRGATVAFMILVNNQAGAAYGPLDHAPWHGLTPT
                     DLVFPFFLFAMGNALAFVMPKMEQQGTPNFLKKVIRRTLLIFVIGLLLNWFPFIRWDD
                     GHLVFKGWTWVNANGGISGIRVMGVLQRIAVCYGLAACTVYFAKFRGAFVICGCLLLI
                     YWAMCLLLGDPADPYSLSGYFGTSVDKAVFGASHMYHGEGVAFDPEGLTSTIPAIVSV
                     VLGYFAGSYIRLKGTSYEMLSHLLVAGILLLFAGYCWGGIFPINKKIWTSSYVLVAGG
                     WACACLSVLIYFIEFRKAHGVWSSFCNVFGKNPLFIFVLSGVVPRALALIHVPDGEGG
                     VTSPLGWFHQYCCKPLSMGRAENASLLYALCLILLYWLLGWWLDKRKIYIRV"
     gene            2419..3228
                     /locus_tag="VGQ51_14950"
     CDS             2419..3228
                     /locus_tag="VGQ51_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV9037931.1"
                     /translation="MKCEAFTETIAKTLNSRGKHIFLAFLFVLALAQTEAQRAAPDVV
                     STKMGSLSIQPIVHASLVLSLPTITIYADPTGGAANYTGIRAPDIILITDIHGDHFDP
                     KTIAAVRTPNTILVVPRVVADKLPDSLKARLVVLANGAHTTQSGITIDAIPMYNLPES
                     PTAMHTKGRGNGYVLGIGGKNIYLSGDTQGIPEMRALKDIDIAFVCMNLPYTMNVDEA
                     ADAVLAFKPHIVYPYHYRGQGGFSDVNAFRTKVEAGDKNIDVRLRNWYPSK"
     gene            complement(3249..3875)
                     /locus_tag="VGQ51_14955"
     CDS             complement(3249..3875)
                     /locus_tag="VGQ51_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9037932.1"
                     /translation="MGPTLFHPMATNLLIAEDHSVTLKGLGLLAEHILKKRSFVTVGS
                     CHQLMKQLSTKTFSHLILDIILADGSALESIPTIRNLYPDLEIMIFSAQPVILYERAL
                     LRYRIRYIVSKDMPELETIKTLRQFLTNERTISHPLSDRSEPFAGMPPREIDVLRFLL
                     KGWKTTQIAETLNLSISTISTLKSRIFQRTQTSNIRELMDLASLHRVS"
     gene            3942..4649
                     /locus_tag="VGQ51_14960"
     CDS             3942..4649
                     /locus_tag="VGQ51_14960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV9037933.1"
                     /translation="MPDETIAGRAKDVFFQHISHDIRGAYFGVASICAIVYEKVVSGE
                     EVPLPVAQSLIEASRYYKYLLDQFLEFTRSKIDAVEEVQEEPFDPLVEVRHIVELNRH
                     LAEEKGIGVDIRVDGAFPRNILTDKWKITRIFYNVFVNALKFSPPGSRILIGFALETG
                     HWILKVSDQGKGIPPEKLERLTLSLQTERPIDNPEGKGLGLFITGYLTRLVGGEVSVE
                     STPETGTSFLFRFPLKS"
     gene            complement(4808..>5680)
                     /locus_tag="VGQ51_14965"
     CDS             complement(4808..>5680)
                     /locus_tag="VGQ51_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV9037934.1"
                     /translation="VFICAVAGVVLLIDWYIHRWAMDLPQLEEKIGEVIDDARKFLRS
                     QGLRVPGGGPHVQPLVAAATAGKMTTSVLTSVIAVVIHLILIVVYMIMLLTMRSHIRV
                     FFLRLVREENKPRLLLIMSRSVRAAQEYIYGMMIIIVYLWIMYSIGFSIVGIRYSIFF
                     AILCGILEIIPFVGNITGSTLTCVMALSQGGGLHMVLGVLICYCFIQFTQFYIISPLV
                     MRAQVNLSLLFTILSLIAGELLWGIPGMFLAIPCLAILKIVCDEVAFLQSFGFLLGRA
                     KPAGPSLFRRRAKK"
BASE COUNT         1338 a         1502 c         1542 g         1298 t
ORIGIN      
        1 gctgatgacc tatatgctgt ataataccga gaatgaaacg atctttctcg aggacgagat
       61 cggctatctt cgcgcctata tcgatctgca gatgctgcgt ttcggaggtg atgtgacggt
      121 cagcttgaat gtccccgaaa accccggcgg tagccgtata gaaccgatgt tgctgatccc
      181 cctggtcgaa aacgccttta aacatggcat cggtctcgta aatgacccgt tgatcctgat
      241 cgatatcgag accccggacg acaagcggct ggtcatgacc gtgaagaata agtatagccc
      301 gctggtccgg gagcgggaga acaaaccggc cggcatcggg ttgagcaatc tgacccgacg
      361 gctcgagatg atgtatcccg ggtgttatga gctggaacga acggataagc tccgcctgga
      421 tgtgggtgaa tcggaacact ggtttatcat tcaactaaac attcccttac aatgatcaat
      481 tgcctggccg tcgacgatga aactccggcg ctggatatcc tggaggacaa tatccggcag
      541 gtgccattcc tgcggctggt aggtaaatgc aagaatgcgt ttgcggccat ggaggtcctg
      601 caacagcaac cggtagacct gcttttcctc gatattgaaa tgccgggtat cagcggtctc
      661 agccttttga aaagccttcc gggcaaaccg attgtcatct tcatcaccgc ctaccggaat
      721 tatgcggtgg agggcttcga cctggatgta ctggattatc tggtcaagcc cgtggccttc
      781 gaccggtttg tgagggcggc gaacaaggcg ctggaatacc agggattcaa actgaaggga
      841 gccgggtccc cggaggccgc gcccgattat ctgttcattc ataccgaata ccagctgacg
      901 aaaatattcc tgaatgagat cgtttatatc gaagcgctcc gtaattatat acgtatccat
      961 ctgacggctg ccgcgaaacc catcctttca aaactgagcc tcaaagccat cgaagaaaga
     1021 ctccctcccg ccaggtttac gcgcgtgcac aaatccttta tcgtaatgct ggataagatc
     1081 gactttgtcc ggaacgatat catccgtatc ggacagcgcg acatcccgtt gagtcgcggc
     1141 tgcagggatg agtttttccg acggatcaat gagatacggt gaccggaggc tgaccggagg
     1201 cggctttttt ttctatcttc accgcctatg aaccaacgct actattcgct ggatgtcttt
     1261 cggggggcga cggtcgcctt tatgatcctc gtcaacaatc aggcgggcgc cgcctatggc
     1321 ccgctggatc atgcgccctg gcatggactg acaccgaccg atctcgtatt ccctttcttt
     1381 ttatttgcca tggggaacgc cctggccttc gtcatgccca aaatggagca gcagggcacc
     1441 cccaacttcc tgaaaaaagt gatccgaagg acgcttctca tctttgtcat agggcttttg
     1501 ctcaattggt tcccattcat acgttgggac gacggacacc tggtttttaa aggctggacc
     1561 tgggtgaatg caaacggcgg tatatccggc atacgggtga tgggcgtatt gcagcgtatc
     1621 gcagtttgct acggcctcgc ggcatgtacg gtctatttcg caaaattcag gggcgccttc
     1681 gtgatctgtg gctgtttgct gctcatctac tgggccatgt gtttattgct cggtgatcct
     1741 gccgatccat acagtctttc cggttacttc gggaccagcg tcgataaagc cgtattcggg
     1801 gcgtcgcata tgtatcatgg agaaggggtg gccttcgatc ccgaagggct gaccagcacg
     1861 atacctgcca tcgtcagcgt agtgctgggc tatttcgccg gcagctatat ccggctcaag
     1921 gggaccagct acgaaatgtt gtcccatctc ctggtggccg gcatcctgct cctgttcgcg
     1981 ggatattgct ggggaggtat tttccccatc aacaaaaaga tctggaccag cagctatgtc
     2041 ctggtagccg gtggctgggc atgcgcctgt ctgtctgtgc tgatctattt catcgagttc
     2101 aggaaggcgc atggtgtatg gagtagtttc tgcaacgtct tcgggaagaa cccgctgttc
     2161 atcttcgtcc tcagcggggt cgtgcctcgc gcactggcgt tgatccatgt gcccgatgga
     2221 gagggcggtg tgaccagccc gctcggatgg ttccatcagt attgctgcaa acccctgtcg
     2281 atgggccggg ccgaaaacgc ctctctgctg tatgcactgt gtctgatcct gctgtactgg
     2341 ctgctcggct ggtggctgga taaaaggaag atctatatcc gcgtataacg gctatcttta
     2401 gcgaacaaac aataaatcat gaaatgcgaa gcattcaccg agaccattgc aaagacatta
     2461 aattcccgag gtaaacatat cttcctcgct tttcttttcg tgctggcatt ggcacagacg
     2521 gaggcgcaac gtgccgctcc cgatgtcgtg tccacgaaaa tgggttcgct tagcatccag
     2581 ccgatagtgc atgccagcct cgtcctgtcg ctgccaacga tcaccatcta tgccgacccc
     2641 accggcggcg cggccaatta taccggtatc cgggcgcccg atatcatcct tatcaccgat
     2701 atccatggag atcatttcga tccgaagacg atagccgcag ttcgcacgcc gaatacgatc
     2761 ctggtcgtac cgagggtcgt ggcggataag ctccccgatt ccctgaaagc gcgcctggtg
     2821 gtcctcgcaa acggggcgca caccacgcaa tccggcatca ccatcgatgc cattcccatg
     2881 tacaatctcc cggaaagccc caccgccatg catacgaagg gccggggcaa cggatatgtg
     2941 ctgggcatcg gcggtaagaa tatctatctc tcgggcgata cgcaaggcat tcccgagatg
     3001 cgggccctga aggatatcga catcgccttc gtctgcatga acctgccgta tacgatgaat
     3061 gtcgatgagg ccgcggatgc cgtactggcg ttcaaaccgc atattgtgta tccctaccat
     3121 taccgcgggc agggcggatt cagcgatgtg aatgcgttca ggacgaaggt ggaggcaggg
     3181 gacaagaata tcgacgtgag attgcggaat tggtatccgt ccaaataatg aaaaaagccg
     3241 cgatacctct aggagacacg atgaagactg gcgagatcca tcagctccct gatattgctg
     3301 gtctgggtcc gttggaagat tctggatttc agcgttgaaa tagtgctgat gctgaggttc
     3361 agggtttcgg cgatctgtgt cgttttccat cccttcagca aaaagcgcaa cacgtctatt
     3421 tcacgcggcg gcatgccggc gaacggctca ctgcggtcgg aaagggggtg agagatagtc
     3481 cgctcattgg tcaggaactg ccgcagggtt ttgatggttt ccagctcggg catgtccttg
     3541 ctgacgatat aacgaatgcg gtatcgcagc agggcccgct cataaagaat gacgggctgc
     3601 gcggagaaga tcatgatttc caggtcggga tacagattcc ggatggtagg gatggactcg
     3661 agcgcgcttc catcggcgag aatgatgtcc agtatcaggt gcgaaaacgt tttcgtcgat
     3721 agttgtttca tcagctgatg gcaggagccc acggttacga aggatcgttt cttcaggata
     3781 tgctccgcaa gcagacccag tcctttgagg gtcacgctat ggtcttcggc aatcaggagg
     3841 ttagtcgcca tgggatggaa aagcgttggt cccataacac tacagggttc ctcttattct
     3901 aaccaatttt ctacaggata tttctatatc ttgccggcca aatgccagac gaaactattg
     3961 ccggccgcgc aaaagatgta ttctttcagc acatatccca tgatatcagg ggtgcttatt
     4021 tcggcgtagc cagcatttgc gctatcgtgt acgaaaaagt ggtttccggg gaagaagtgc
     4081 ccctgcccgt ggcccaaagc ctgatagagg cgtcccgata ctacaaatac ctgctcgatc
     4141 aatttctcga attcacccgc agcaaaatag atgcagtcga agaagtgcag gaggaaccct
     4201 tcgaccccct ggtagaggtc cgtcatatcg tggaattgaa ccgtcacctg gccgaagaga
     4261 agggaattgg cgtggatata cgcgtcgatg gcgcatttcc ccggaacatc ctgacggaca
     4321 aatggaagat cacacgcatc ttttacaatg tcttcgtcaa tgccttaaaa ttttcccctc
     4381 cgggttcccg catcctgatc ggctttgcgc ttgaaaccgg ccattggata ctgaaggtat
     4441 cggaccaggg gaagggtatc cctccggaaa agctggaacg actcaccctc tcattgcaga
     4501 ccgaaaggcc gatcgacaat cccgaaggga agggcctcgg tctttttatc acgggctacc
     4561 tgaccagact ggtggggggc gaagtgtccg tcgaatctac ccccgaaacg ggcaccagtt
     4621 tcctgtttcg gtttccgcta aaatcctgaa aaatctctac aaactgctcg aaggaggaat
     4681 cctatcttac gcataagttt aatggaacag caggaacttg aaaaaaagtt ccgacgacga
     4741 cctgttttta cagggagtcc aataggataa ggtttaaaga aaccagggta tttccatatc
     4801 ctggttttta ttttttcgct ctcctccgaa aaagagaagg tcccgcgggc ttcgcccggc
     4861 caagtaggaa gccgaaggac tgcaggaacg ccacctcgtc acagacgatc ttcaggatcg
     4921 caagacaggg aatggccagg aacatccccg ggatacccca caaaagctca cccgcgataa
     4981 ggctcaggat ggtaaacaga agcgagaggt tgacttgcgc cctcatcacc agcggcgaaa
     5041 tgatataaaa ctgcgtaaac tgtataaagc aataacagat aagcacgccc agcaccatgt
     5101 ggagaccgcc gccctgcgac agggccatca cgcaggtgag cgtcgatccg gtgatattgc
     5161 ctacgaacgg aatgatctcg agtataccgc aaaggatggc gaagaatatc gaatacctga
     5221 tgccgacgat ggaaaagccg atgctataca tgatccacag atagacgatg atgatcatca
     5281 ttccgtagat gtattcctgg gccgcgcgga ccgaccggct catgatcagc aggagacggg
     5341 gtttgttctc ctcgcgtacc agccgcagga aaaagacccg gatatgcgat cgcattgtca
     5401 aaagcatgat catatagacc acgataagga tcaggtggat gaccacggcg atcacgcttg
     5461 taaggaccga agtggtcatc tttcccgctg tcgcggcggc aaccagcggc tggacgtgcg
     5521 gtcctccgcc gggaacccgt aatccctgcg atctcaggaa tttacgggca tcgtcgatca
     5581 cctcgccgat cttctcttcc agctgcggca gatccatcgc ccagcgatgg atataccagt
     5641 cgatcagcag gaccactccc gcgacggcac aaatgaatac
//
LOCUS       DASYSV010000486         8380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2022527, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000486 DASYSV010000000
VERSION     DASYSV010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022527"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..2251
                     /locus_tag="VGQ51_14970"
     CDS             23..2251
                     /locus_tag="VGQ51_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037935.1"
                     /translation="MKKLSIILWLLTGLGAWCQVKKTARTTGPKHLHVRMLAKAYADS
                     IVVRWAPMDPVAWLMGNDSGYRIVRIDYSDRAHPVTTVLSPGLRPLPLEQMMATIGPN
                     DKYAGVAAQALYGKDFQMTKEAPYGFEKKVKQAHEALNFRYSFTMQAADFSPATASAI
                     ALRWVDRNVRKGSDYIYVITVCGATKDYVVDSSAAFVVDNNATPIPPPDGLQGFGFDR
                     KSELHWNRRQRGNFSAYDIGRSDDGGKTWVVLNKAPYLSPDKVPQVPGAVKGSKDTTI
                     LKIEALMRDHQVFMDSLPRDYHPYLYRVRGIDAFAEWSPWSAPVAVEGRDLTPPVAPV
                     IDSVRNTMGSQLRIVWSQRVSSPDLAGYYISKGNSVKGPFYPLTKYMLPKETRVFTDS
                     AGVAHLPNYYVIVAVDTAKNVAASGAFPGYLTDTVPPAAPVRVAGSIDSMGVVRLHWA
                     ANHEPDLKGYKVYFAYGARDQFAQVTHVLLADTGFVDTVSMRSLNRRVWYSVVAVDNS
                     NNHSAYSAPAMLKKLVVVPPSAPLAGTITVAARRVTVEWIESRSEGAAGYEIARMRGV
                     PNTPGAGQPKTPGAGQPNTPVAGPPAQYALVGKMAQDWNRRSLFFDDTISVNTDYYYT
                     ARTIDSTGVRSEWSSPVHAVYHATDSLAAPSGLQVRLDDKRHHVRFSWQYKGKGDYFF
                     VVYRSFNHGALSAWHSFDKDVSSGEDDEAGNGGYDYAIKVVYRDRSAVSAMSTPVHIT
                     IP"
     gene            2263..3879
                     /locus_tag="VGQ51_14975"
     CDS             2263..3879
                     /locus_tag="VGQ51_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037936.1"
                     /translation="MKSKICSLVIAALLVLAACSLLTSCAKEGPQGIPGATGPAGKDG
                     SQIYAGTGAPSPSLGQDGDYYLDKSTGNLYGPKTDAGWGTPLNLTNGQGGSGQQGPAG
                     PQGPSGPQGPQGPSGPQGPQGPSGPQGSQGAAGATGKSGSQIFSGSGAPATTLGNIGD
                     YYLDKTNYELYGPKTANGWGTPMLLRGAQGPQGPAGPSGVAGSQIFAGTGAPAANLGN
                     IGDYYIDRSTGNFYGPKTANGWGQPIALGGGSGTQGPPGPQGPPGPQGPPGPQGPPGP
                     QGPQGPQGPQGPQGPQGPQGPQGSQGAQGATGAAGAAGKAGSVIYSGSGAPAANIGAV
                     GDYYIDDTHAIMYGPKTSTGWPATGLSLQVNNTITYDFVNRVQHNLPYNWVVPIVLAG
                     GGPIWEEIMLPYNNSVAQTTGFTIPQTVVDNGVVLAYIRYFLGGPNDTATDAGMTAWI
                     ELPASFVTNYTINGVSAQAQVSLTSNFDDAGFYIYLSATSSNIAAGYAQNHIPAVIRV
                     VEIPAAQINTITSLKPGAIPAQHGKLVTLN"
     gene            3885..4208
                     /locus_tag="VGQ51_14980"
     CDS             3885..4208
                     /locus_tag="VGQ51_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037937.1"
                     /translation="MGLFDQTPTGPANDPLNTNRIKTDIETVIQSMASGNPDTEKIKE
                     AGSDLLNTAASILSNQGIDQLTGGGAPEEAAGGIFKRLRDGLGLDSHALDGLKQAAEQ
                     LKNKL"
     gene            4220..4651
                     /locus_tag="VGQ51_14985"
     CDS             4220..4651
                     /locus_tag="VGQ51_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037938.1"
                     /translation="MKSWKKWALAALILLPACKKDSKDRSSSASGWIDGFWTYKEDPA
                     EDYWNANVLFKTDGTFRMYQALSLNDTALGPALADTAGQVVTFGTYTVSGTTVTMKYT
                     EFNIINFTFTGSVNGTHNIIVGNLSDNNPGDAEPLWYLTKP"
     gene            4664..5032
                     /locus_tag="VGQ51_14990"
     CDS             4664..5032
                     /locus_tag="VGQ51_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037939.1"
                     /translation="MKKNLLVFPLFAAIGLGLTLAGHNLVGHWNASYGNGPKGHMVFR
                     NNGTFEATFTGQKWKVGGQYKVTGNTVAFADSSCGLQYWGKYNVTWLSDDSARASVLE
                     DTCAGRRANGDGAVMVRAKR"
     gene            5310..7751
                     /locus_tag="VGQ51_14995"
     CDS             5310..7751
                     /locus_tag="VGQ51_14995"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037940.1"
                     /translation="MLRSFLHIALRNFAKRRGYSILNLLGLTIGISCCLLIFEYVGYE
                     RSFDSYNPDAKNIFRVQENDYENNHFAARWATTSPAVGPALKKDFPAIQSFCRLYRAD
                     GQLTNPSNNVRFHEEKSYAADAAAIGILDLHLVKGDPATALIGPAKVILNKTTAKKYF
                     GNDNPVGKTLYNEDGGRRRPVEVTGVFADLPDNSHIRPDILFSYPTLKAWMNFGNTTE
                     DFTQTAWSWNDYYTYIRLKPGSDWHTLQARLPDFMDRHYNSFPAHKANKDYNLLQLFP
                     LTDIHLDSHFDEEAEANGDGSSVSFLFLIAFFIAVIAWINYINLATARSLERAREVGV
                     RKVLGALRTHLIRQFMVESLLLNGLACVLAIAITWSLNPLFNQLTGRTIRTIPLTYCE
                     IFAGLFLAGTLLSGIYPAFVLSRYHPVTVLKGLFKNAAGGQFLRKALIIGQFAASILL
                     IAGTIIVYKQVHFMRSRELGVDISQTLVVNGVSTLSDSIYTATYPAFRQDILSLKNVS
                     AVTGTTDIPGTEITWATDWQRLRSNPKKNYMIHHLGVDYDYFKYYGLKIIAGRSFSKD
                     YPTDKKGVILNETALKLLGYARPEDAIGETLHADQSQIDTVHVIGVAADFHQQGLQKA
                     IDPLAIVLRLPEPSYYSVRVNGNMPHTIAAIKAVWQRYFPTDAFRYFFLDEHFERQYA
                     ENERFGTVFGLFAGLAIFIACLGLLGLSAYNVIQRTKEIGIRKVLGASVQHLLYILSR
                     DFLLLVALAFVIAVPVTWWAMSSWLREFAYRIDIGWWTFVVAGILAFGIALITVGLQA
                     LKATLANPVKSLRTE"
     gene            7840..8127
                     /locus_tag="VGQ51_15000"
     CDS             7840..8127
                     /locus_tag="VGQ51_15000"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9037941.1"
                     /translation="MTTSGSSSFLETSSAFVDPDFFSIVAIIISCLGLFGLATYSAQI
                     RTKEIGIRRVLGATVIQVTSLLARDFITLIAFSFFIAAPVAGWLMSAFAPR"
     gene            complement(8185..>8380)
                     /locus_tag="VGQ51_15005"
     CDS             complement(8185..>8380)
                     /locus_tag="VGQ51_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037942.1"
                     /translation="LNAQINLTHEISIFGIVDNLTNSLGLTEGNPRAGEIESAEANSY
                     IFLARPLLGRSFKVSLRYKF"
BASE COUNT         1906 a         2376 c         2338 g         1760 t
ORIGIN      
        1 atacaatcat gaaagtacaa aaatgaaaaa gttatcgatc atattatggt tactgacggg
       61 gctcggggca tggtgccagg ttaaaaagac ggccaggacc accggaccaa agcatctgca
      121 tgtgcggatg ttggccaaag catatgccga ctcgatcgtc gtccggtggg cgccgatgga
      181 tcctgtcgcc tggttgatgg ggaatgattc gggttaccgg atcgtgcgga tcgattacag
      241 tgaccgcgcg catcccgtga ctacggtctt gagcccgggg ttgcgtccat tgccgctgga
      301 acaaatgatg gcgacgatcg gccccaacga taaatatgcg ggggttgcgg cgcaggcctt
      361 gtatggcaag gactttcaga tgacgaagga ggcgccgtac gggtttgaaa aaaaggtgaa
      421 acaagcgcat gaagcgttga acttccggta ttcgtttacg atgcaggcag cagatttctc
      481 gcccgcgact gcaagcgcca ttgcgctaag atgggtagac agaaatgtga gaaagggatc
      541 ggactatatc tatgtgatca cggtgtgcgg cgctacgaag gactatgtgg tcgattcatc
      601 ggcggccttt gtcgtcgata acaacgcgac cccgatcccg ccgccggacg ggttgcaggg
      661 ttttgggttc gaccgcaaat ccgagcttca ctggaaccgc agacagcggg gcaatttcag
      721 cgcgtatgac atcgggcgca gcgatgacgg aggcaagacc tgggtagtct tgaacaaggc
      781 gccttatctc agccccgata aggtaccgca ggtcccgggt gcggtaaaag gcagcaagga
      841 tacgacgatt ttaaagatcg aggccctcat gcgagatcac caggtcttta tggacagttt
      901 gccgcgggac tatcatccct atctgtaccg ggtgcggggg atcgacgcat ttgcagagtg
      961 gagcccgtgg tcggcacccg tcgcagtaga gggcagagat ctgacgccgc ctgtggcgcc
     1021 ggtgatcgat tcggttcgga acacgatggg atcgcaattg cggatagtat ggagtcagcg
     1081 cgtgtcgagc cccgacctcg ccgggtatta tatcagcaaa gggaatagcg tcaaaggtcc
     1141 attctatcca ctgaccaaat acatgttacc gaaagagaca cgggttttta ccgattctgc
     1201 cggggttgcg catttgccca attattatgt gatcgtcgca gtcgacacgg caaagaatgt
     1261 cgctgcttcg ggtgctttcc cgggttatct cacggatacg gtcccgccgg cggcgccggt
     1321 gcgggtggcc gggtcgatcg attcgatggg tgtagtgcgg ctgcattggg cggccaatca
     1381 tgaacccgat ctgaaagggt acaaagtata tttcgcgtat ggcgcgaggg accagtttgc
     1441 gcaggtgaca cacgtgttat tggccgacac cggttttgtc gataccgttt caatgcggtc
     1501 gttgaacagg cgggtatggt actccgtggt agcggtcgac aacagcaata accattcggc
     1561 ctattcggcg ccggcgatgt tgaagaagct ggtggtcgtg ccgccgtcgg cgccccttgc
     1621 gggaaccatt acggttgccg cccgccgggt gacggtcgaa tggatcgaaa gccggagcga
     1681 gggtgctgcc ggctatgaga tcgcgcggat gcgcggtgtg ccgaatacgc caggtgccgg
     1741 tcaaccgaag acgccaggtg ccggtcaacc gaatacgccg gtcgccggtc cacctgctca
     1801 atatgcattg gtcggaaaga tggcccagga ctggaacaga cggtctctct tttttgacga
     1861 cacgatcagc gtcaacaccg attattatta tacggcgcga acgatagaca gtaccggtgt
     1921 gcggtccgaa tggtcgtcac cggtgcatgc ggtataccat gcgacagaca gcctggcggc
     1981 tccctccggt ttgcaggtcc ggctggatga caagcgtcat catgtccggt ttagctggca
     2041 gtataagggc aaaggcgatt atttttttgt ggtctaccgg agcttcaatc acggtgcatt
     2101 gagcgcctgg cactcctttg ataaggacgt atcctcgggg gaggacgatg aggccggtaa
     2161 cggcggatat gactatgcca tcaaagtggt ataccgcgac cggtcagcag tttccgcgat
     2221 gagtacacct gttcatataa cgattcccta aaaatagcag tcatgaaaag taagatttgt
     2281 tctcttgtga tagcggcgtt gctggtgctg gcggcttgtt cgctgctcac atcctgcgca
     2341 aaggaggggc cgcagggtat tcctggcgca acaggacccg cgggtaagga tggcagtcag
     2401 atatatgccg gtaccggagc gccgtcccca tcgctgggac aggacggcga ttattatctc
     2461 gacaagagta cgggcaattt gtatggtccc aagaccgatg cgggctgggg taccccgttg
     2521 aatctgacca atggacaggg tgggtccggt cagcagggtc ctgccgggcc acaaggacca
     2581 tccggaccac aaggaccaca gggaccatcg gggccacagg gaccgcaagg accatcggga
     2641 cctcagggat cacagggtgc ggcaggggct accggtaaaa gcggaagcca gatcttcagt
     2701 ggcagcggag cgccggctac gacccttggg aatatcggag attattatct cgacaagacc
     2761 aactatgagt tgtatgggcc gaagacggca aatggctggg gaacgccgat gctgttgcgg
     2821 ggggcacagg gaccacaggg accggccggg ccctcgggtg tagcagggag tcagatcttt
     2881 gccgggaccg gggcaccggc cgccaatctc ggcaatatcg gagactacta tatcgacagg
     2941 agtaccggca atttctatgg tccgaaaact gctaacggtt ggggccagcc gatagcgctc
     3001 ggcggtggca gtggtaccca gggccccccc ggcccacagg gacctcccgg cccacaggga
     3061 cctcccggcc cacagggccc ccccggccca caaggccctc aaggccccca aggtccacaa
     3121 ggcccccaag gtccacaagg accacagggc ccgcaagggt cacagggagc tcagggtgcc
     3181 accggcgccg caggcgcggc aggcaaagcg ggcagcgtca tctacagcgg cagcggtgcg
     3241 cccgcggcca atatcggggc agtaggcgac tattatatcg atgacacaca tgcgatcatg
     3301 tatgggccga agacaagtac cgggtggccg gcgacgggtt tgtcgctgca ggtcaacaat
     3361 accattacgt atgattttgt gaacagggtg cagcacaatc ttccctacaa ctgggtggtg
     3421 ccgatcgttt tagcaggggg cggtccgata tgggaagaga tcatgcttcc ttataataat
     3481 tcggtggcgc aaaccacggg tttcacgatc ccgcagacgg tggtcgacaa tggggttgtc
     3541 ctggcgtata tccggtattt tctcgggggg ccgaatgaca cggcgacgga cgccggcatg
     3601 acagcctgga tagagctgcc ggcatcgttt gttaccaact acacgattaa cggcgtatcc
     3661 gcgcaggcgc aggtcagcct cacgtcgaat ttcgacgatg cgggtttcta tatctatcta
     3721 agcgccacgt catcgaatat cgccgcaggt tatgcgcaaa accatatccc ggcggtcatc
     3781 cgggtagtcg agatccctgc ggcccagatc aataccatca cgtcgctgaa gcccggagcg
     3841 attccggcgc agcatggcaa acttgtcact ttaaactaaa ctatatggga ctttttgatc
     3901 aaacaccgac cgggccagcg aatgacccgt tgaacactaa ccggatcaag accgatatcg
     3961 agactgtgat acagtcgatg gccagcggga atccggacac ggaaaaaata aaagaagcgg
     4021 gcagcgatct gctaaacacc gcggcatcga tcctttcgaa ccaggggatc gatcagctga
     4081 cgggtggcgg cgcccctgag gaggccgcgg gcggaatatt caaacggctg cgggacgggc
     4141 tcgggctcga tagtcatgcc ctggacggat tgaagcaagc cgctgaacaa ttaaaaaaca
     4201 aactttaaaa ccttttgcaa tgaagagctg gaaaaaatgg gcattggcag cgctcatact
     4261 gctgcccgcc tgtaaaaagg acagcaaaga ccggtcttcg tccgcgagcg gctggatcga
     4321 tgggttctgg acctataaag aagaccccgc ggaagactat tggaacgcca acgtgctttt
     4381 caaaaccgac gggaccttcc ggatgtacca ggcgctttca ttgaacgata ctgcattggg
     4441 ccccgcactg gcggacacgg ccggccaggt agtgacgttc gggacttata cggtcagcgg
     4501 tacgacggtg acgatgaagt ataccgagtt taatatcatc aacttcacgt ttaccggttc
     4561 cgtcaacggc acacacaata tcatcgtcgg aaacctttcg gataacaacc ccggcgacgc
     4621 cgagccgctg tggtatttga caaaaccata ataaacaaaa cacatgaaaa agaatcttct
     4681 tgttttcccg ctattcgcgg cgatcggttt gggattgact ttggcagggc ataatctggt
     4741 cgggcattgg aatgcctctt atggaaacgg gccaaaaggt catatggtgt tcaggaataa
     4801 cggaaccttc gaggccactt tcaccggcca aaagtggaag gtgggaggcc aatataaggt
     4861 gaccgggaat accgtggcct tcgccgatag ttcatgcggt ctccaatatt ggggaaaata
     4921 caatgtaaca tggctttccg atgactcggc cagggcgtcc gtcctggaag acacctgcgc
     4981 gggcaggcgg gccaatgggg acggtgcggt catggtgaga gccaaaagat agaagtcatg
     5041 gtgatccgcg gagctaatcc tgtcttccct acttttaaat cagcgagttg tatacgactc
     5101 gcttttttgt tctctacggc taacccgccc cacccgatgc cccccaccca tcccccggca
     5161 gccgccgccc cccgcttgta tcaccaccgt ccacgccttg tatcaaaacc gaacatcccg
     5221 gcaacgcaaa taccctaact gaatgaaaac cacccccatc tcatatcggc accccgtttg
     5281 ccgccagacc cgcataaacc ctccctccca tgctcaggtc cttcctgcac atcgcacttc
     5341 gcaacttcgc caagcgaagg gggtactcga tcctcaacct gctcggcttg accatcggta
     5401 tctcctgttg tctcctgatc ttcgaatacg tcggctacga gcggtccttc gacagctata
     5461 acccggacgc caaaaacatc tttcgcgttc aggagaatga ttacgagaat aaccacttcg
     5521 cggcaagatg ggccacgacc tcaccggcgg tcggaccggc cctaaaaaag gacttcccgg
     5581 cgatccagtc cttttgccgc ctctataggg cagacggtca attgaccaac ccttcgaata
     5641 acgtccggtt ccacgaggag aaaagctatg ccgccgacgc ggcagcgatc gggatactgg
     5701 acctgcacct cgtgaaagga gatccggcca cagcgctcat cggtccggca aaagtcatcc
     5761 tcaacaaaac caccgcaaaa aaatatttcg gtaacgacaa ccccgtcggc aagacgctct
     5821 acaatgaaga cggaggcaga cgccggcctg tcgaagtcac cggtgttttt gcagacctcc
     5881 ctgacaactc acatataagg cccgatatcc tgttctcgta tccgacgctg aaggcctgga
     5941 tgaacttcgg taacaccacc gaagacttca cccagacagc ctggtcctgg aacgattact
     6001 atacctatat ccggctgaag ccgggctccg actggcatac cctccaggcc agactgcctg
     6061 atttcatgga ccgccactac aattcattcc ccgcacacaa ggccaacaaa gactataacc
     6121 ttctccaact cttcccactc accgatattc atctcgattc gcattttgac gaggaggccg
     6181 aagccaacgg cgatggaagc tccgtctctt tcctgttcct gatcgccttc ttcattgccg
     6241 tgatcgcctg gatcaattat atcaatctgg cgaccgcccg ctcgcttgag cgcgcccgcg
     6301 aagtcggggt acgcaaggtc ctcggcgcgc tgcgtaccca tctgatccga cagttcatgg
     6361 tcgaaagtct cctgctaaat ggcctggcat gtgtactggc catcgccatc acgtggtcgc
     6421 tcaaccctct gttcaaccaa ctcaccggcc gcaccatccg cactatccca ctcacctact
     6481 gtgagatctt tgccggactt tttctggcgg gcaccctgct tagcggcatt tatccagctt
     6541 tcgttctctc ccgctatcat cctgtaaccg tcctgaaagg gctgttcaag aatgccgccg
     6601 gcggccagtt tctccgcaag gcgctcatca tcggccaatt tgcggcctcc atcctcctca
     6661 ttgcaggtac gatcatcgtc tataagcagg tgcacttcat gaggagccgc gagttggggg
     6721 tcgatatcag ccaaaccctg gtggtcaacg gcgtcagcac gctttcggac tcgatctaca
     6781 ccgccactta tcccgcattc cggcaggata ttctcagcct gaagaacgtg agtgcggtta
     6841 cgggtaccac ggatatcccc ggcaccgaga tcacctgggc gaccgactgg caacggcttc
     6901 gtagcaatcc aaaaaagaat tacatgatcc accaccttgg tgtcgactat gattatttta
     6961 aatactacgg gttgaagatc atcgccggcc gcagcttctc gaaagactat cccaccgata
     7021 aaaaaggcgt catcctcaac gagaccgccc tgaaattgtt gggttatgca aggccggaag
     7081 acgcgatagg agagaccctt cacgcagatc agagccagat cgatacggtc catgtcatcg
     7141 gcgtcgccgc ggactttcac cagcagggtc tgcagaaagc gatcgatccg ctggccatcg
     7201 tattgcgtct tccggagccg tcttactact cggtccgcgt caatggcaac atgcctcaca
     7261 cgatcgcggc gatcaaggcc gtctggcaac gctacttccc gacagacgcc ttccggtatt
     7321 tctttctcga cgaacacttc gagagacaat atgccgagaa cgagcggttt ggtaccgtct
     7381 tcggtctctt tgccggcctc gccatcttca tcgcctgcct gggcctgcta gggctctctg
     7441 catataacgt gatccagcgt acgaaagaga tcggcatccg gaaagtgctc ggagcatcgg
     7501 tgcaacacct gctctatatc ctctctcggg atttcctgct actggtcgcg ctcgcctttg
     7561 tgatcgccgt gcccgtgaca tggtgggcca tgagcagctg gctgcgggag tttgcttacc
     7621 ggatcgatat cggatggtgg acctttgtcg tggcgggtat ccttgccttc ggcatcgcgc
     7681 tcatcaccgt cggcctccag gcgctcaaag cgacgctcgc caatcccgta aaaagtttaa
     7741 gaaccgaatg atacgcctca acctcctaac tgcctggcgc gacaatctat tcccggggtc
     7801 gcccaggcaa ccctcattct tccgcgctgg gagagactat tgacaacttc cgggtcctcg
     7861 agcttcctcg agacctcatc cgccttcgtc gacccggact tcttctccat tgtagccatt
     7921 atcatttcct gtctcggtct gtttggcctg gctacgtact ctgcccagat ccgcacgaaa
     7981 gagatcggca tccgtcgcgt gctgggtgca accgttatcc aggtgaccag cctgctggca
     8041 agggacttca ttacactcat tgctttctct tttttcatcg cggcaccggt cgccggttgg
     8101 ctgatgagcg cattcgcgcc gcgctgaccg atcctgtcag gagcctccgg agtgaatgac
     8161 acggcaaaaa gcctcttttc actatcagaa cttataccgt aaagaaactt taaaactcct
     8221 tcctagcagc gggcgcgcca gaaatatata cgaattcgct tctgcgcttt cgatctcacc
     8281 tgcgcgtgga ttaccttccg taagccctaa ggaattggta agattgtcga caattccaaa
     8341 gatcgaaatt tcatgggtaa ggtttatctg ggcgtttaaa
//
LOCUS       DASYSV010000487         8870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_355626, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000487 DASYSV010000000
VERSION     DASYSV010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355626"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VGQ51_15010"
     CDS             complement(<1..304)
                     /locus_tag="VGQ51_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037943.1"
                     /translation="MNSKLQLSTRAIAFLSSLAMVAVLYLPIWRIELSAPQYPEGLVL
                     KIYSNRIGGDVNVVNGLNHYIGMHTLHTRDFVEFVVLPYIIGGFAVLGLLVTLIGRK"
     gene            complement(360..2348)
                     /gene="nosZ"
                     /locus_tag="VGQ51_15015"
     CDS             complement(360..2348)
                     /gene="nosZ"
                     /locus_tag="VGQ51_15015"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HEV9037944.1"
                     /translation="MNSRQRFIALVALTGVIASLQSCKPRNAAGAISADAAEKTYVAP
                     GKYDEFYNFVSGGFNGQVSVYGLPSGRLLKIIPVFSVFPENGYGYSEETKAMLNTSQG
                     FVPWDDQHHLELSQTDGQPNGKWLFANANNTPRVARIDLATFKTEEILELPNSAGNHS
                     SPFCTENTEYIVAGTRFSVPIGDNQDVPINSYKKNFHGTVSFIKVDSGTGRLQLAFQI
                     LLPGVNFDLSHAGKGPSHDWFFFSCYNTEQAHSLLEVNASQRDKDFIMAVNWKKAEEY
                     VREHKGALKQVSYIHNIYDEKTHSAATSTENQVVVLDPTELKDFVYFIPCPKSPHGCD
                     VDPTGEYIVGSGKLAALIPVFSFSKLQQAIAGKQFDGDYGGIPVIKYEAALYGEVPKP
                     GLGPLHTEFDGRGNAYTSMFVSSEVVKWSIKDLKVLDRVPTYYSVGHLCIPGGDSKNP
                     YGKYLIAYNKITKDRYLPTGPELAQSAQLYDISGDKMEMILDFPTVGEPHYAQALPAD
                     MISKNSVKYYRIEDNGNPYAAKGEKQAKVVREGNKVHVYITAIRSHFVPDNIEGVRMG
                     DEVYFHVTNLEQDWDIPHGFAVKGAANAEILIMPGETCTLKWIPTKTGVFPIYCTDFC
                     SALHQEMQGYVRVSPAGSNTPLVFGTNLADSSAPVAKK"
     gene            complement(2407..2871)
                     /locus_tag="VGQ51_15020"
     CDS             complement(2407..2871)
                     /locus_tag="VGQ51_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV9037945.1"
                     /translation="MKRYLVAILILAFAYSCSNNSSSSNAAVSAPAASDNASGYDSLR
                     GSGKFTHVDLAATLDPKMVDAGKSIYDLKCSACHKLTSEKLVGPGWLGVTGRHKPEWI
                     MNFVTNTDEMIDKDPKAQAMLQICMVRMPNQHLSDDDARHILEFMRNNDQGK"
     gene            3004..4248
                     /locus_tag="VGQ51_15025"
     CDS             3004..4248
                     /locus_tag="VGQ51_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037946.1"
                     /translation="MAEPLYVRMEDTHRLKIWTRLSLINLLIVSVLGVLLRYKGAFYF
                     PAVDYKYLLNAHSHFAFSGWVTTALFVALTYILVRSGVPLSRSYRYQFRLNQLASFGM
                     LVSFMIQGYGPISIFFSALSVIFSYWFSIRYWLDIRKSRMPPPVTAAIRLALVFLVLS
                     TAGPFLLAYSTSHGVRSTSFYYNSIYLYLHFQYNGWFSFGVLALLFWTLFAGSFPINK
                     RLAVWTIGLMGAACVPAYCLSLLWTAPPLWIRVTAGTAALVQLSALLLLVSLLWKAFV
                     AWRGRPSRLAVILWGLSFSALALKIVLQALSVIPTLGRFAFSFRPVIIAYLHLVLLAF
                     VTFFLIGFFSAENMLDIRGPIKRAGLAAFITGVFANECILLLQSLMATRGIAWMSAPY
                     FLFAAALCMFAGLLPLTTGRIK"
     gene            complement(4241..6745)
                     /locus_tag="VGQ51_15030"
     CDS             complement(4241..6745)
                     /locus_tag="VGQ51_15030"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV9037947.1"
                     /translation="MSTAIQAEEGALPEGLSSEEALSRRTRYGKNILGGPGGGYALRA
                     LRDILIEPMFLVLLFAGVLYFLLNKPREGVLMLLALVFVGAISFYQDMKRHRALSALR
                     DYTEPRVRTVRDGQLKVLHGWELVPEDVMLLEEGEKIPADAVVLTAHDLSVNESVVTG
                     ESLPVDKDGSEGRNKLLLGSLVNTGTCYARVTDTGKRTFLGRMGKKMAEIPEERTALQ
                     RQINGFVRVMAIFGVTAFLLVWGINFRHSGNLAQSFLLGLTLAMTAIPEEIPIAFSSF
                     LALGGWRMAKLGIIVSKPAIMDYLGTTTTICLDKTGTLTMNKMSVSWLYDFTSDVEAE
                     TSSGRLSQSRVMWYGRLASEPEPFDAMERAIVEEFVKRPDATDWSRFVMIREYPLEGR
                     PPMMTHVYRDGRGDVVVAAKGGPERILAVCRLSEGMRDEAMRRVAMMAAKGYRVLAVA
                     SAEWPGGAFPERQDDFDWKFEGFIALFDPPKPGLREVFKAWRQAGMRVKILSGDYPET
                     VVNIASAGGLSVGKIVTGNMVTEMSDEELRKRVGDAVIYARMFPDAKLRVIQALKEHG
                     EVVAMTGDGVNDGPALKAAHIGIAMGKAGTDLARQAADLVVSDDDLSHITEAILRGRT
                     VHNNLKKAIRYLIGIHIPIILTVALPLLLGWKYTAVFTPIHIVFLELIMGPTCSIFYE
                     REPAEAGIMNRPPAMAGNGFFTRREAVFTVLQGLVAASGMLAIYFYFMDKGASLAYVR
                     TLVFLAIILMNLVLTFVSRSFSHPVLASFRNRNGLAAVVLAMSAGFIAIVIGVPFVRG
                     LFGMVPVMPVDFARIFAVVVVCGLCMEGMKRAGKAI"
     gene            6865..7779
                     /locus_tag="VGQ51_15035"
     CDS             6865..7779
                     /locus_tag="VGQ51_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV9037948.1"
                     /translation="MKPARTPTVLVPIDFSPASGAGLRFALQWASQQRISLVCLHIVY
                     LLRPTSWSDQRYRNYAQAEKKRHFQQLNDFVGKIITQAATGKPDISFVTIEAISPDIA
                     LQQYCRRRKDIDYICIGTNGARGLKKLFGTHTGNLITHSNTPVIAIPASYRRSAITRI
                     LYASDLADYTPEIQRLIRFAGPLKANVRTLHLNYLGEIWLNEDSLKTVLKHQFHYPVD
                     VSIVHADPNLSVTRNLQRQITAFKPSMVVLYTQHRDSVIQRILYPSVAERLSFQAKTP
                     LLVFPKTGKDSSGAKLSTAKTTALLRAK"
     gene            7785..8681
                     /locus_tag="VGQ51_15040"
     CDS             7785..8681
                     /locus_tag="VGQ51_15040"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV9037949.1"
                     /translation="MPAIKHYLINGVTYWRIVMAPLIVALAIFGQEQPFKWLLLISFL
                     TDAVDGKLARHYHIASKRGAMLDSLGDDLTVLAGFSGLLAFHLDFVLAHIVSLSFLAI
                     TCLLQFAVAIRKYGKPTAFHTILAKIAAVLQGLFLLTFFFWPGIPLILFYTAVGVTCL
                     QLLEETIMVTLIPEWKSDVKGLYWYLKERSASQSHRHRPRNKTPDTLTQDQTPDTLIE
                     NKTPDTLIDHLNLKYGRLHPLSKLWNATRPSGPSGHRKGRLEEHYLLPVLLSGWRIQP
                     PGNDDRGNADPRTRTTRTDPRH"
BASE COUNT         2062 a         2712 c         2009 g         2087 t
ORIGIN      
        1 tcttgcggcc gatcagcgtg accagcaaac ccaacaccgc aaatccaccg atgatatagg
       61 gcagcaccac aaactccaca aagtcccgtg tatgcagcgt atgcatcccg atatagtggt
      121 taagcccatt gaccacattg acatcccccc cgatgcgatt gctgtatatc ttcagcacga
      181 gcccttcggg atattgcggt gcggacaact cgatccgcca gatcggcaga tatagcaccg
      241 ccaccatcgc cagcgacgaa aggaacgcaa tggcccgggt cgataattgt agtttgctgt
      301 tcatgggatc atttgtagtt gatgctcatg gcgtcatttt catttcgccg ttcatcgcat
      361 cattttttgg cgacaggtgc gcttgaatcc gccaggttgg ttccaaaaac cagcggcgta
      421 ttgcttcccg ccggcgatac ccggacatac ccctgcatct cctggtgaag cgcactacag
      481 aagtctgtac aatagatcgg gaataccccg gtctttgtcg gtatccattt cagtgtacac
      541 gtctcacccg gcatgatcag tatctcggca ttggcggcgc cttttacagc aaatccgtgc
      601 gggatatccc aatcctgctc caggttggta acatggaaat atacttcgtc gcccatccgc
      661 actccctcga tattatccgg aacaaaatgg ctgcggatgg cggtaatgta cacatgcacc
      721 ttattgccct cacgaactac cttagcctgt ttttcgccct tggcagcgta aggattcccg
      781 ttgtcttcga tccggtagta tttcacggaa ttcttagaga tcatatccgc cggcagagcc
      841 tgcgcgtagt gcggttcacc gacggtcgga aaatccagga tcatctccat cttgtccccc
      901 gaaatatcat acaattgcgc actctgcgcc agttccggcc ctgtagggag ataccggtcc
      961 ttggtgatct tattataagc gatcaggtat ttcccgtagg ggttcttcga atcgccgccg
     1021 ggaatacaca agtgacccac cgaatagtaa gtgggtacgc gatccagcac cttcaggtcc
     1081 ttgatgctcc atttcaccac ttcggaagaa acgaacatcg aagtataggc attccccctc
     1141 ccgtcgaact cggtatgcaa tgggccgagc cccggtttcg gcacttcacc ataaagtgcc
     1201 gcctcatatt tgatcaccgg tatcccgcca tagtcgccgt caaactgctt cccggctatt
     1261 gcctgctgca gcttactgaa cgaaaatacc ggtatcaacg ccgccagctt tccgctcccg
     1321 acgatgtatt cccccgtcgg gtcgacatcg catccgtgcg gagacttggg acagggaatg
     1381 aaatacacga aatcctttag ttccgtggga tccaatacca ccacctggtt ctccgtggag
     1441 gtagcagcag aatgggtctt ttcgtcatat atattatgta tatacgaaac ctgtttaagc
     1501 gcacccttgt gctcccgaac atattcttcc gcctttttcc agttgacggc catgatgaag
     1561 tccttatcac gctgtgaagc attcacctcc agcagcgaat gcgcctgttc cgtattatag
     1621 caggagaaaa agaaccagtc atggctcggg cccttacccg catggctgag atcgaaattg
     1681 acgccgggca gcagtatctg gaaggccagt tgaagacggc ccgtcccgct gtccaccttg
     1741 ataaagctca ccgtcccatg gaaattcttt ttataagaat tgatcggcac atcctggttg
     1801 tccccgatcg gtacgctaaa ccgcgtaccc gccacgatat attccgtgtt ttcggtgcaa
     1861 aacggagaag agtgatttcc ggcgctattg ggcagctcga ggatctcttc cgtcttaaag
     1921 gtcgccagat cgatccttgc aacccgcggt gtattgttgg cattggcgaa aagccacttc
     1981 ccgttaggct gtccatcggt ttgcgaaagc tccaggtggt gctgatcgtc ccagggcacg
     2041 aagccttgcg aagtattaag catggccttt gtttcctcgc tgtagccata accgttctcc
     2101 gggaataccg agaacaccgg gatgatcttc agcaaccggc ctgatggcag accgtataca
     2161 gatacctggc cgttgaatcc gccggaaacg aaattgtaga attcgtcata tttaccgggg
     2221 gcaacatagg ttttttcggc ggcatcggcg ctgatggcgc ctgctgcatt gcgtggcttg
     2281 caggattgca gactggcgat gactcctgtc agagccacca gtgcgataaa gcgttgtcga
     2341 ctgttcattt tgcgttcaat ttatgttgat gggttatttg ccttggttct tcttgagatg
     2401 ccggggttat ttgccctggt cgttgttccg catgaattcc aggatatgcc gggcatcgtc
     2461 atcggacaga tgttggttgg gcattcggac catgcatatc tgcagcatcg cctgcgcctt
     2521 cggatcctta tcgatcattt cgtctgtatt ggtcacgaaa ttcatgatcc attcgggttt
     2581 gtgccgcccg gtcaccccca gccagcccgg cccgaccagc ttttcggacg tgagcttatg
     2641 acaggcagag catttcaagt catagatgct ttttccggca tccaccattt tcggatcgag
     2701 cgtcgcggcc agatcgacgt gcgtgaactt acccgagccc cttaaggagt cgtacccgga
     2761 ggcgttatcg gaagccgcgg gtgcggagac tgcagcattg gaagaggaag aattgttgga
     2821 gcaggaatag gcgaaagcga ggatcaggat cgctaccagg tatcttttca ttgttgaatt
     2881 ttttacggca caaggttacg gccggccaat atgtcaacac atgactttga tcagccggtt
     2941 accaaacaat gatcatatta tcccggtacc ggtcacctta ggtttgcaaa aagggatacc
     3001 tttatggccg aacccctgta tgtaagaatg gaagataccc accgtctgaa aatctggaca
     3061 cgactaagtc tgatcaatct tctcatcgtc tccgtcctcg gggtattatt aagatacaag
     3121 ggtgctttct atttccccgc cgtagactat aaatatctcc ttaatgctca ctcccatttt
     3181 gccttctcgg gatgggtgac caccgccctg ttcgttgccc ttacctatat cctcgttcga
     3241 agcggcgtcc cattgtcacg ctcctatcgt tatcaattcc ggctcaacca gcttgccagt
     3301 ttcggcatgc tcgtcagctt catgatccag ggatatggcc cgatatcgat cttcttttca
     3361 gccttatcgg tcatcttttc ctattggttc agcatacggt attggctcga tatccgcaaa
     3421 agccggatgc ccccgccggt aacggcggcc atccgactgg ccctcgtctt cctcgtactg
     3481 tccaccgcag gaccatttct actcgcatat agcacatccc atggggtgcg ctccacctcc
     3541 ttctactaca attccatcta tctgtatctt catttccagt acaacggctg gtttagcttc
     3601 ggcgttctgg cgctgctctt ttggacctta ttcgccggct ccttcccaat aaataaaagg
     3661 ctggccgtct ggaccatcgg cttgatgggc gccgcctgtg ttccggccta ttgcctatca
     3721 cttctatgga cggcccctcc gttatggatc cgggtcacgg ccggcactgc tgcacttgtc
     3781 cagctgtcag cactgcttct cctggtcagc ctcctctgga aagcatttgt tgcctggcgc
     3841 ggcagaccat ccaggcttgc agtcatctta tggggactat ccttctcggc actggcgctt
     3901 aagatcgtcc tccaggcact ctcggtaatc cccacactgg gccgatttgc cttttctttc
     3961 cggccggtga tcattgccta tctgcatctc gtcttgctgg cattcgtaac cttcttcctg
     4021 atcggcttct tttccgcaga gaacatgctc gacatccgcg gcccgatcaa aagagccggt
     4081 cttgccgcat ttattaccgg cgtattcgcc aatgaatgta tcctcctttt acaaagcctg
     4141 atggctaccc gcggtatcgc ctggatgagc gctccttatt tcctgtttgc ggcagccctc
     4201 tgcatgtttg ctggtctgtt gcctttgaca acaggacgca tcaaatagcc ttcccagccc
     4261 tcttcattcc ttccatacac aacccgcata ccactaccac cgcaaaaatc cgcgcgaaat
     4321 ctaccggcat caccggcacc ataccaaaaa gcccgcgcac gaaaggcact cctatcacaa
     4381 tggctatgaa tcccgccgac attgccaata ccaccgccgc cagaccgttc ctattccgaa
     4441 aactggcgag caccgggtgc gagaacgacc ggctgacaaa ggtcagtacc aaattcatca
     4501 ggataatggc cagaaatact agcgtccgca cgtacgcgag cgaagctcct ttgtccatga
     4561 agtaaaaata aatagccagc atccccgacg ccgccaccag cccctgcaac accgtaaaga
     4621 cagcttcccg acgggtaaaa aagccattcc ccgccatcgc aggcggtcta ttcatgatgc
     4681 ccgcttccgc cggctcccgc tcataaaaga tcgaacaggt aggacccatg atcagttcga
     4741 ggaaaacgat atgaatggga gtgaatacag ccgtatattt ccatccgagt agcaacggca
     4801 gcgcaaccgt aaggattatc ggtatgtgaa tgccgatcag gtaccggatg gcctttttta
     4861 aattgttgtg gaccgttctt ccacgcagga ttgcctccgt aatgtggctc agatcatcat
     4921 cgctgaccac cagatcagcc gcctgccgtg ccaggtcagt acccgccttc cccatcgcga
     4981 tgccgatatg cgcagctttc aacgccgggc catcgttcac tccatcaccc gtcatcgcga
     5041 cgacctcgcc gtgttccttc aatgcctgaa tgacccttag cttcgcgtca gggaacatac
     5101 gggcatagat aaccgcgtcc ccgacccgtt tgcgcagttc ctcatcggac atctcagtta
     5161 ccatattccc tgtcacgatc tttccgactg aaagcccccc ggcagatgca atgttcacca
     5221 ccgtctccgg ataatccccc gaaaggatct tcacccgcat tccagcctgc cgccaggctt
     5281 taaacacctc gcgaagaccc ggcttggggg gatcgaaaag agcgataaat ccttcaaatt
     5341 tccaatcgaa atcgtcctga cgttcgggga acgcaccgcc aggccactca gccgaagcaa
     5401 ccgccagcac cctatatcct tttgccgcca tcatggcaac gcgacgcatg gcctcatccc
     5461 gcatcccttc agacagcctg cataccgcca ggatccgctc cggccctccc tttgctgcca
     5521 ctaccacatc tccgcggcca tcgcgataca catgcgtcat catcggagga cgtccttcca
     5581 gcgggtactc gcgaatcatc acaaatcgac tccagtccgt cgcatccggc cgtttcacga
     5641 actcctccac aatggcacgc tccatcgcat cgaagggctc cggttccgac gcaagtcgtc
     5701 cataccacat gacacgcgac tgcgacagac ggcccgaaga cgtctccgcc tcaacatcgg
     5761 aggtaaaatc gtacagccaa ctcacactca tcttattcat agtcaacgtt cccgttttgt
     5821 ccaaacagat cgttgtcgtc gtacccaggt aatccatgat ggcgggcttg ctgacgataa
     5881 tccccagttt ggccatgcgc catccgccca gcgcaagaaa agaggaaaag gcaataggta
     5941 tttcttccgg tattgctgtc atcgcgagtg tcaagccgag aaggaaactt tgggccagat
     6001 taccggaatg ccggaaattg atgccccaaa ccaacaaaaa agcggtgacg ccgaagatcg
     6061 ccattacccg gacaaatcca ttgatctgac gttgcaatgc cgttcgctcc tcaggaattt
     6121 cggccatttt ctttcccatt cttccgagaa acgttcgttt ccccgtgtcc gtgacccgcg
     6181 cgtagcaagt tcctgtattc accagggatc ccagcaaaag cttattcctt ccttccgaac
     6241 cgtccttatc taccggcagc gattcaccgg taactaccga ctcgttgacc gaaagatcat
     6301 gcgccgtcaa aaccaccgca tccgccggga tcttttctcc ttcttccagc aacatgacat
     6361 cttcaggcac cagttcccat ccatgcaaga cctttaattg accatcacga acagtccgga
     6421 cccgaggctc cgtatagtcg cgaagcgcac tcagcgcccg gtgcctcttc atgtcctggt
     6481 aaaagctgat agcaccgacg aaaaccagcg ccagcagcat cagcaccccc tcccggggtt
     6541 tgttcagtaa gaaatagagg actccagcga acagcaacac caggaacatc ggttcgatca
     6601 aaatgtcccg caacgcccgg agcgcatatc cgcctcccgg cccgcccagt atattcttac
     6661 cgtaacgcgt tctccgtgat aaagcctcct ccgagctcaa accctcgggc aaagccccct
     6721 cttctgcttg aatagcggta ctcatccgaa aataaaggta gcccgtcgct cagccgcgtc
     6781 ctttgacaaa gatcagtctc gcacctgaca cacatcaggg atggacgccc ccacctccgg
     6841 taattttaaa caaattccag caacatgaaa cccgcaagaa caccgacggt cctcgtcccc
     6901 atcgacttct cgcctgcatc gggggccgga ctacgattcg cccttcaatg ggccagccag
     6961 caacgcattt ccctggtctg tctgcacatc gtatacttac tccgtcccac ctcatggagc
     7021 gaccaacggt atcgcaacta tgcgcaggct gaaaaaaaac gtcattttca acagttgaat
     7081 gacttcgtgg gtaagatcat tacacaggca gccactggta aaccggacat ctccttcgtg
     7141 accatcgaag cgatatcgcc cgacattgca ctgcaacaat attgccgaag acgcaaggac
     7201 atcgattata tctgcatagg cacaaatggc gctcgtggcc tgaaaaaact gttcggcact
     7261 cataccggca atcttattac gcattcgaac acgcccgtga tcgccattcc tgcctcctac
     7321 agaagaagcg ccatcacgag aatactctac gcaagcgatc ttgccgatta caccccggag
     7381 atccagcggc tgatccgatt tgccggtccc ctgaaagcaa atgtccgcac cttgcatctc
     7441 aactacctgg gagaaatatg gctcaacgaa gacagcctga aaaccgtcct caaacaccaa
     7501 tttcattatc ccgtggacgt cagcattgtg catgcagacc cgaatctttc ggtcaccagg
     7561 aacctgcagc ggcaaataac ggccttcaaa ccctccatgg tcgtcctata cactcaacac
     7621 cgagactcgg tcattcaacg cattctctat cccagcgtag ccgaaaggct cagctttcag
     7681 gcaaaaactc ccttgctggt ctttccaaag acaggcaaag attcctccgg cgccaaactg
     7741 agcaccgcaa agacaacagc gttactacgc gcaaaatgaa caccatgcca gccatcaaac
     7801 actatctgat caatggagtc acctattggc gcatcgtcat ggctcccctg atagtcgcgc
     7861 tggccatatt cggtcaggag caacctttca aatggctgct attgatcagc ttcctcaccg
     7921 atgccgttga cggcaagctg gcccggcatt accatattgc cagcaaacgc ggcgcaatgc
     7981 tcgattccct tggtgatgac ctcactgtcc tcgcaggttt ttcagggctg ctggcctttc
     8041 atcttgactt tgtactcgca cacatcgtca gcctatcctt tctggccatc acttgtctgc
     8101 tgcaattcgc cgttgctatc cgtaaatatg gtaaacccac ggctttccac accatcctcg
     8161 ccaagatcgc cgccgtacta caaggcttat tcctgctgac cttcttcttt tggccgggca
     8221 ttccgctgat cctcttctat accgcagtcg gggttacttg tcttcaattg ctggaggaga
     8281 ccataatggt gaccctcatc cccgaatgga aatccgacgt caaaggcctt tactggtatc
     8341 ttaaagaaag atcggcctcc cagtcccacc gccatcgacc ccggaacaaa acacccgaca
     8401 ccctcaccca ggaccaaaca cccgacaccc tcatcgagaa caaaacaccc gacaccctca
     8461 ttgatcattt aaatcttaaa tatggaaggc tacatccatt gtcaaagctg tggaatgcca
     8521 ctcgaccatc cggaccttct gggcaccgaa aaggacggct cgaagagcac tatttattgc
     8581 ctgtattgtt atcgggatgg cgaattcagc cgcccggaaa tgacgatcga ggaaatgcag
     8641 acccacgtac gcgaacaact cgaacagacc cacgccacta aagcgacgat cgaaaaggca
     8701 cagaggcgtc ttccgcatct gttgcggtgg caaggtgtcc cggccattca tcattgtgcg
     8761 gattggcatt agcccatatt caagctgaca atatgaacaa cttaatcgac ctcatcaaac
     8821 aacgccaatc ctcgcggacg cccttcgacc cggaaaggcc ggtatcgaaa
//
LOCUS       DASYSV010000488         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_355756, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000488 DASYSV010000000
VERSION     DASYSV010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355756"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VGQ51_15045"
     CDS             <1..759
                     /locus_tag="VGQ51_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEV9037950.1"
                     /translation="GGRLLWALSPFIFLTVIIILWGLAPVKNWLTANGTLQFAVPGLH
                     NAIREYDGPLVAHIFRFNYLSAAGTAILIAAIVSLLFSGLTLRQGMAVFGQTLRQLAF
                     PILTIAVVLAFAYLVNDSGISQTIAQCLASTGILFPFFAPVLGWLGVFITGSDTSSNA
                     LFGRLQSATASSIGVDPIVTVGANLSGGVIGKMISPQSIAVAAAAGGLVGRESALFRF
                     TIKHSFILLSIICCLVLAQAYWLKWIIPVYIRHL"
     gene            756..1718
                     /locus_tag="VGQ51_15050"
     CDS             756..1718
                     /locus_tag="VGQ51_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEV9037951.1"
                     /translation="MSSIIVLDGHTLNPGDLSWEPWQQYGDIIVYERTSPEEIVHRCA
                     AADIVLTNKVPLNADTLAKLPLLQYIGVTATGYNIIDIGAAHRHRIVVTNVPSYGTAS
                     VAQMTFALLLELTLRVQRHSDSVMEGKWSRSPDFCFWDYPLVGLAGKTIGIIGMGNIG
                     GKVADIATAFDMSILGASRTRTDQSHRRNFSWATIPELLQRSDVVSIHCPLFPETRGL
                     INRETLSLMKRSAFLLNTSRGPIIVEEDLAEALNTGIIAGAGIDVLTTEPPVKGSSLF
                     GARNCIITPHISWATKEARARLMKTAFDNLAAYKSGHPVNLCTS"
     gene            1706..2830
                     /locus_tag="VGQ51_15055"
     CDS             1706..2830
                     /locus_tag="VGQ51_15055"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV9037952.1"
                     /translation="MHILIAPNAFKNSLSAAAASSAILQGLQQSRLGFTGECFPVGDG
                     GDNTGDLLIQRLHGRRLPAEARDPLGRKITAHFGIVGSETAIIDMSTASGLRLLDQKK
                     LDPLHATSAGTGDLIRVALDHRVSHILLGVGGSATVDGGMGILTALGIRFRDDAAREL
                     TTPESLTGLDSIDVSGLDPRLKDTRLTILCDVETPPLGPTGSAAVFGPQKGASPEDVV
                     LLESVLQRLCAIIRQSTGRDISQLPFGGAAGAAAAGLYGLLGARLVKGIDYFLQLTDF
                     DAALGRSDLVVTGEGAIDEQTLQGKAPYGVAIRAKKRGLSVIAFTGRVPDDPAPLHPY
                     FDEIIPINPPLMSLPEALASAAANLEATARILGSRLATTQ"
     gene            complement(2801..>3299)
                     /locus_tag="VGQ51_15060"
     CDS             complement(2801..>3299)
                     /locus_tag="VGQ51_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEV9037953.1"
                     /translation="GIRALSRYHLEHPFVFNHAGQEYRVGYLPADSDSGMFGGNSNWR
                     GPVWMPANLLLIEALQRFHDYYGDDFRIEYPTGSGRKLSFLEIGVELSKRLSRLFLRD
                     EKGRRAIFGDNDKMQRDPHFKDYILFYEYFDGENGRGLGASHQTGWTGLVTILLLLGR
                     RKTTS"
BASE COUNT          701 a         1017 c          861 g          720 t
ORIGIN      
        1 ggaggccgcc ttctctgggc cctgagcccc ttcatcttcc ttaccgtcat catcatcctg
       61 tggggactcg cacctgtaaa gaactggctt accgccaatg gcactcttca attcgccgta
      121 cccggcttgc ataatgccat ccgcgaatac gatggtccgc tcgtagcgca catcttccgg
      181 ttcaattacc tgtcggctgc tggcaccgcc atccttatcg ccgcgatcgt atcgctgctg
      241 ttttcgggtt tgacgctcag acagggcatg gccgtcttcg gacaaacgct tcggcaactg
      301 gccttcccga tactgacgat cgccgtcgtc ctggccttcg cctatttggt caacgattcc
      361 ggtatttcgc aaacgatcgc acaatgtctg gccagcaccg gtatcctgtt tccctttttt
      421 gcgccggtac tgggctggct gggtgtcttc attaccggat cggacacctc atccaatgcc
      481 ctcttcggca gactgcagtc ggcaaccgcc tcctccatcg gcgtcgatcc gatcgtcacg
      541 gtgggcgcca atctttccgg cggtgtcatc ggcaaaatga tctctccgca atccatcgcg
      601 gtggctgccg ccgcgggcgg cctcgtcgga cgcgaatcgg cccttttcag attcacgatc
      661 aaacatagtt ttatcttgct ttccatcatc tgctgtttgg ttttagcgca ggcctattgg
      721 ctgaaatgga taattcctgt ttatattcgc catttatgag ctccatcatc gtactcgacg
      781 gccacaccct caaccccggc gacctctcat gggagccgtg gcaacaatac ggagacatca
      841 tcgtatatga acgaacctct cccgaagaga tcgtgcatcg ctgcgcggct gcggatatcg
      901 tattgaccaa taaagtcccg ctgaacgccg ataccctggc aaaacttcca ttactgcaat
      961 atatcggcgt caccgccacc gggtacaaca ttatcgacat cggggccgcg catcgccacc
     1021 gcatcgtcgt caccaatgtc cccagctatg gcaccgcctc ggtcgcacaa atgacctttg
     1081 ccctcctgct cgaactgacc ctgagggtgc agcggcacag cgattcggtg atggaaggga
     1141 aatggtcgcg gtcgcccgat ttttgttttt gggattatcc gctggtcggg ctggccggaa
     1201 agaccatcgg catcatcgga atgggcaaca tcggcggcaa ggtagccgac atcgccacgg
     1261 ccttcgatat gtccatactg ggcgccagcc gaacccggac cgaccagtcg catcgtcgca
     1321 atttttcctg ggctactatc ccggaattgc tccaacggtc ggatgtcgtc agcatccatt
     1381 gccccctatt ccccgagacc aggggtctta ttaacaggga aaccctatca ttgatgaaac
     1441 gcagcgcctt tttattgaat acctcgagag gaccgatcat cgtggaagag gaccttgccg
     1501 aggcattgaa caccggcatc atcgcgggcg caggcatcga cgtactcacc accgagccac
     1561 cagtcaaggg tagctcgctt ttcggcgcca ggaactgtat cataaccccg catatctcct
     1621 gggctacgaa ggaggcgaga gcccgtctca tgaaaaccgc gttcgataac ctggcagcct
     1681 ataaatccgg tcatccggta aatctatgca catcctgatc gctcccaacg ccttcaagaa
     1741 cagcctgtcc gccgccgctg cctccagtgc cattttgcaa ggtctgcaac aaagccggct
     1801 cggtttcacg ggagaatgtt tccccgtcgg tgacggaggt gacaatacgg gtgatctgct
     1861 cattcaacgc ctgcatggcc gccgtctccc cgccgaagcc cgcgaccccc tcggccgaaa
     1921 aataaccgcg catttcggga tagttggaag cgaaacagcc attatcgata tgtccaccgc
     1981 ttccggactt cgcctcctgg accaaaaaaa actcgacccg ctgcacgcca cgtccgcggg
     2041 cacaggcgat cttatccgtg ttgcactgga tcatcgggtc agccatatcc tcctgggcgt
     2101 cggcggatcg gcgactgtcg acggaggcat gggtatcctg acagcactgg gcatacgatt
     2161 cagagacgac gcggcgcgcg aactgaccac acccgaaagt ttaaccggtc tggattccat
     2221 cgatgtatcg ggtctcgacc cccggctgaa agacacccgg ctaaccatac tatgcgatgt
     2281 ggagaccccg ccgctgggac ccaccggctc tgcagccgtc ttcggacccc aaaaaggagc
     2341 ttctcccgaa gatgtggtcc ttctcgaatc cgtcttgcag cgactgtgtg cgatcatccg
     2401 acaatccacg ggcagggata tttcgcaact gccttttgga ggagccgcgg gtgcggccgc
     2461 cgcaggactc tatggtctgc tgggagcccg gctcgtcaaa gggatcgatt atttcttaca
     2521 gctgacggat ttcgatgcgg ccctgggtcg aagcgatctt gtcgtcacag gtgaaggtgc
     2581 catagatgaa caaacgctgc aaggcaaggc cccatatggt gtagccatcc gcgccaaaaa
     2641 aagaggatta tccgttattg catttacggg ccgtgtaccg gacgacccgg ccccgcttca
     2701 cccgtatttc gatgagatca ttccgatcaa cccgccactg atgtccttgc cggaagcgtt
     2761 agcctccgcc gcggccaacc tcgaggccac cgccaggatt ttaggaagtc gtcttgcgac
     2821 gacccagtaa aagcagaatg gtcaccaaac ccgtccatcc cgtctggtgc gaggcgccca
     2881 atccgcgtcc attctctccg tcgaaatatt catagaagag gatataatcc ttgaaatgcg
     2941 gatcacgctg catcttgtca ttgtcgccga aaattgcacg ccgtcctttt tcatcccgta
     3001 ggaaaagacg cgagagtcgt ttggacagct cgacgccgat ttcgagaaag gaaagctttc
     3061 gacccgaacc tgtgggatat tcgatcctga aatcatctcc ataatagtca tgaaaccgtt
     3121 gtaatgcctc gatcaggaga agattggcgg gcatccagac ggggccgcgc cagttggaat
     3181 tgcccccaaa catgccgctg tcggaatcgg ccggcagata accgacgcgg tattcctgac
     3241 cggcatggtt gaaaacgaag gggtgctcca ggtggtagcg cgacagggcg cggatacca
//
LOCUS       DASYSV010000489         7564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_739054, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000489 DASYSV010000000
VERSION     DASYSV010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739054"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VGQ51_15065"
     CDS             <1..258
                     /locus_tag="VGQ51_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037954.1"
                     /translation="ALLEKGCYYLIQENEEGPIGLIKVNMETDHCMYVSKYADTTLAV
                     WKKKTDPIFDILELLGDDKVKTWESIYNDSQDTYNYEEDEE"
     gene            complement(237..935)
                     /locus_tag="VGQ51_15070"
     CDS             complement(237..935)
                     /locus_tag="VGQ51_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037955.1"
                     /translation="MKTGIYSVLCAFITNAAIGACFVPFLLLGLKKVRQMNTFWILGF
                     YWFLNGLVNLRALKVFSAGAIREFLQHLSDYYDLADIPLALLIFAVSVRGKTRKQLLL
                     VLLGFVAGESLLIGQKGYAFSWPIVVGVGVSLIVGFSITGLWLYLKKMEHDRFENSMA
                     FIHGAFLFAYGTYLIIYLLFYLRASGTYSETDSSLLYYTSLLFSAAITSAGMFSYGWR
                     WTRTSNPRYSSSSS"
     gene            1091..3043
                     /gene="dnaG"
                     /locus_tag="VGQ51_15075"
     CDS             1091..3043
                     /gene="dnaG"
                     /locus_tag="VGQ51_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV9037956.1"
                     /translation="MIAQETIQQILSRIDIIEIVGSFVKLKKRGANYLGLCPFHNEKT
                     PSFTVSPAKEIYKCFGCGRSGNTISFLMEHEKYSYAEALRWLAARYNVEIEEKELNPE
                     QRLQQQAAESLYIINGFAQKFFSDVLFQTDEGQDIGLSYLKERGFTETIIRKFQLGYC
                     PEERDSFARAALAAQYNPENLRKSGLVVLRDERLVDNYRGRVIFPVHNQTGKVIGFGA
                     RVIRNNDRAPKYINTPENEIYVKSRILYGSWMARQAIDKQDECLLVEGYTDVISLHQA
                     GIENVVASGGTSLTIDQLRLIKKYTRHLTILYDGDNAGIKAALRGLDMALEESLDVKL
                     VLIPDKEDPDSYVRRLGAQAFRDFIADNKKDFILFQLEVALRDAADDTNKKAAVVNQV
                     AETIARINKLDGFTRQEDYIRQSAGLLHIEEAGLTNLVNKYIRERIEKQENRTAARTA
                     PPPSDTDPTTRPDNTPPPDDESIGLLFRHEQNERGMIRDLLEYGLRAWDEKTLVADYI
                     LSEAGELIDLIDNKRLLRIFDLYKTWYEQGLEPSTRNFLYHEDQELSTLAVSIMDLHY
                     EISPNWKEHFEGKIFSPDELYRENVLSTMGYLKLRKIKRLIEENQKDMNKSQPPEELK
                     LQMETHQHLKELEKKLVHLLGTVILK"
     gene            complement(3057..3620)
                     /locus_tag="VGQ51_15080"
     CDS             complement(3057..3620)
                     /locus_tag="VGQ51_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV9037957.1"
                     /translation="MALFSRTTLITDLLDRTEVLKVNTRAFGRLTDEQLRQSPEPGRW
                     SISEIYAHLNLCLDQSIRLILSRVTLAPDSPSDVYRSGLLGDWIYDRAMPRPDGSVLK
                     LKARKAWCADTGAIDAREELGLFEHHCDALDDILRHASTKDLRRISIPFFPPLVQLRL
                     GDMLRFLVAHGERHLLQAQRVMIPQVV"
     gene            complement(3781..4239)
                     /locus_tag="VGQ51_15085"
     CDS             complement(3781..4239)
                     /locus_tag="VGQ51_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037958.1"
                     /translation="MSQTLLEKLQLTDERNLLIQGLPSSIEKQFVKLAFAKNLTPLLK
                     SKKIDFALVFAINEQQLGMIVQEVLPALRPEGKFWVAYPKPGSKIVTTLNRERSWGCI
                     TGAGYEVVRDVPLDHVWTAVRFRKTNPAAGFRTPLRGSGARSRRVGAAHQ"
     gene            complement(4310..4978)
                     /gene="plsY"
                     /locus_tag="VGQ51_15090"
     CDS             complement(4310..4978)
                     /gene="plsY"
                     /locus_tag="VGQ51_15090"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV9037959.1"
                     /translation="MKEVLLILSAYLIGSIPTAVWVSRAFFGIDIREYGSGNAGATNT
                     FRVLGSKWGAIVMSVDVIKGVIATSLYILIPYYLHDEAERTNFMVGLGLAAVTGHIFP
                     IWAEFRGGKGVATLFGMILAIQPLAAVCCVAVFLLVLYLTRFVSLSSILASVAFAVFI
                     LYIFDAKETLYRGFAIAVALLVVLTHQKNISRLLRGSENKVPILRYRDRRRQRRHVTD
                     RDKE"
     gene            complement(5053..5886)
                     /gene="prmA"
                     /locus_tag="VGQ51_15095"
     CDS             complement(5053..5886)
                     /gene="prmA"
                     /locus_tag="VGQ51_15095"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV9037960.1"
                     /translation="MTFNKAALRPFFMHIGITIKANAELQEVLIALLAEMGYDGFEQQ
                     EGLLMAYVSETGFDEHRLKSLGLGYEITRIAEKNWNEEWEKSFQPVVIEGFCAVRAHF
                     HDPIPGVAYELVITPKMSFGTGHHATTLMMLQAMQGLPLDGKRVLDFGTGTGVLAILA
                     EKLGAAGVEAIDCDDWSIDNARENAEANGCRRINIQKGEVVHGGPFDIILANINKHVI
                     LEELSAMEQQLTTGGVILLSGLLKEDSEDIENEAAKTNFSISLRMTKENWILLKSEKM
                     K"
     gene            complement(5894..>7564)
                     /gene="pnp"
                     /locus_tag="VGQ51_15100"
     CDS             complement(5894..>7564)
                     /gene="pnp"
                     /locus_tag="VGQ51_15100"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV9037961.1"
                     /translation="ADMEFMIAATDKNLMMVEGEAKECSEEDLVRALEMAHDAIRVHI
                     RAQDELRSKAGVAGKREYTRPVQNDELQQKVTAFTTDRINQISRAGSSKHERGDAFDK
                     LKEDLIASLGEEVTDADKKLAKKYYADLQWELVRNMILDDRIRLDGRQLDQVRPLAME
                     IDPLPTPHGSALFTRGETQSLTTVTLGTPLDELLVESAAKSVDSKFFLHYNFPPFSTG
                     EVKMMRGQSRREVGHGNLAMRSLRQMMPGSDYPYTVRVVSDILESNGSSSMATVCAGS
                     LALMDAGVPIPKHVSGVAMGLITRPADNKNAILTDILGDEDHLGDMDFKVTGTREGIC
                     GVQMDIKVDGLSMNTMREALEQARKGRLHILDAMYECIPAAHADVKPHAPRMVKIFID
                     KEFIGAVIGPQGKIIQEIQRETGTTINLEEVGNFGEVSIFSPAKEGLDKAVTWIKSIV
                     AQPEVGSVYEAKVKSVMPYGAFVEFLPGKQGLLHISEISHKRLETMDGVLKEGDTVKV
                     KLIGLDQKTGKFKLSRKVLMPKPEGSGSGHGGGHGHAKSPAGGHEDQE"
BASE COUNT         1851 a         2088 c         1958 g         1667 t
ORIGIN      
        1 gcgctgttgg aaaaaggatg ctattacctc attcaggaaa atgaggaagg acctattgga
       61 ttgatcaagg tgaacatgga gacggaccat tgcatgtacg tttctaaata cgccgatacc
      121 acattggcgg tctggaagaa aaaaacggat cccatcttcg atatattgga gctgttgggg
      181 gatgacaagg tgaagacctg ggaatcgatc tataacgaca gccaggatac ctataactac
      241 gaagaagacg aagaataacg cgggttagac gtccttgtcc agcgccatcc ataactgaac
      301 atgcccgcgc tcgtgatggc ggcggaaaac agcagactgg tataatacag caacgagctg
      361 tccgtttcgc tgtaagtgcc ggatgccctc aaataaaaca acagatagat gatcaggtag
      421 gtgccgtatg cgaaaaggaa ggccccgtgg ataaaggcca tcgaattctc gaaccggtcg
      481 tgctccatct tcttgagata tagccaaagc cccgttatgc tgaacccgac gatcagcgaa
      541 acgccgaccc ctacgacgat gggccacgaa aaggcatacc ctttctgtcc gatcagcagg
      601 gattctcccg caacaaaacc gagcaagacg agcagcagct gttttcgggt ctttccccga
      661 acggagacgg caaagatcag cagggccagt ggtatgtcgg ccagatcgta atagtcgctc
      721 agatgttgaa ggaattcgcg gatggctccg gcgctgaata ctttcaatgc gcgaagattg
      781 acgagcccgt taaggaacca gtaaaaaccg aggatccaga aggtattcat ctgccgcacc
      841 tttttcagac ccagtaacaa aaatgggacg aaacaggcgc cgatggcagc gttggtgatg
      901 aaggcgcaga gcacggaata aatcccggtt ttcaaaggat gaacgtttac atataaatcg
      961 gaaaaaagag gactttatta taggtaaaaa tactcagttt tatacataat ttacggtagt
     1021 tttcgcccac tgcgcgggag ccgccgtgat tggcgtcaat ccccccggtt tgacgtaaat
     1081 tgagccccga atgatcgctc aggaaaccat acaacaaata ctcagccgga tcgatatcat
     1141 cgagatcgtg ggcagcttcg tgaagctgaa aaagcgcggt gcgaattatc ttgggctttg
     1201 tccttttcac aacgaaaaaa cgccttcctt cacagttagt cctgccaagg agatctacaa
     1261 atgtttcggc tgcggtcgca gcggtaacac catcagcttc ctgatggaac acgagaaata
     1321 ttcctatgcg gaggccttgc ggtggctggc ggcacggtac aatgtcgaga tcgaagagaa
     1381 agagctcaac ccggagcagc ggctccagca acaggcggcg gagagccttt acatcatcaa
     1441 cgggtttgct caaaagttct tttccgacgt actcttccag acggatgagg ggcaggatat
     1501 cggtctcagc tatctgaaag aacgtggttt caccgaaacg atcatacgga aattccagct
     1561 gggctattgc ccggaagaac gcgattcttt cgcccgggct gcgctggcgg cccagtacaa
     1621 cccggaaaac ctccggaaaa gcggtctggt ggtccttcgt gacgaaagac tggtagacaa
     1681 ttaccgcggt cgcgtcattt tccccgtcca caaccagacg ggaaaggtca tcgggttcgg
     1741 ggcacgcgtc atccggaaca acgaccgggc gcccaaatac atcaataccc ccgagaacga
     1801 gatctatgta aagagcagga tactttacgg atcatggatg gcccgccagg ccatcgacaa
     1861 acaggacgag tgtctgctgg tggaaggata tacggacgtg atatcgctgc accaggccgg
     1921 tatcgagaac gtggttgcca gcggaggcac gagtcttacg atcgaccagc tccggctgat
     1981 caaaaaatac acccgccatc tgaccatcct ctacgatggc gacaatgcgg gcatcaaggc
     2041 ggccctgcgg ggtctggata tggcgctgga agagagtctc gacgtcaagc tcgtactgat
     2101 ccccgacaaa gaagaccccg acagctatgt gcgcagactg ggagcccagg ccttccggga
     2161 ttttatcgcc gacaacaaaa aggatttcat ccttttccag ctggaggttg cgctgcggga
     2221 tgccgccgat gatacgaata aaaaagccgc cgttgtcaac caggtagccg agacgattgc
     2281 ccgtatcaac aagctggatg gttttacccg ccaggaagat tatatccggc aatctgccgg
     2341 attgctgcat atcgaagaag ccggtctcac caatcttgtc aataaataca tccgggaaag
     2401 gatcgaaaaa caggaaaacc ggaccgccgc aaggaccgct ccacccccct ccgataccga
     2461 cccgactacc cgcccggata atacgccgcc gcctgacgac gaatccatcg gcctgctgtt
     2521 ccgtcatgaa caaaacgaac gcggaatgat ccgtgacctg ctggaatatg gtcttcgcgc
     2581 ctgggacgag aaaaccctgg tagccgacta catcctttcc gaagcgggcg aactcatcga
     2641 cctgatcgat aacaaacggc tgctcaggat attcgacctc tataagacct ggtacgaaca
     2701 gggactggaa ccctccacgc gaaatttcct ttaccacgaa gaccaggaat tgagcaccct
     2761 tgccgtatcc atcatggatc tccattacga gatcagcccc aattggaaag aacatttcga
     2821 aggcaagatc ttttcccccg acgaacttta cagggagaat gtgctttcca ccatgggcta
     2881 tctcaaactc agaaagatca agagattgat cgaagagaac cagaaagaca tgaacaaatc
     2941 ccagccgccg gaagaactga aattacaaat ggagacgcat cagcatctga aggaacttga
     3001 aaaaaaattg gtgcatctat tgggaacggt catccttaaa tagcccctct ctcgcttcaa
     3061 accacctgtg gtatcatcac ccgttgggcc tgcagtaaat gcctttcccc gtgcgccacg
     3121 agaaatcgaa gcatatcccc caaccgcaat tgcacaagag ggggaaaaaa tgggatggat
     3181 atccggcgca gatccttggt ggacgcatga cggagaatat cgtccagcgc gtcgcaatgg
     3241 tgctcgaaca gaccgagctc ttccctcgca tcgatggccc ccgtatcggc gcaccaggcc
     3301 ttcctggctt ttagtttcaa aacgctgcca tccggtcggg gcatggcccg atcgtagatc
     3361 cagtcgccca gcaagccgct gcgatacaca tcggacggac tatccggagc gagcgtgacg
     3421 cgcgacagga tcagacgtat ggattgatcg agacagagat tgaggtgtgc gtagatctcg
     3481 gagatgctcc atctgccggg ctccggagat tgccgtagct gttcgtcggt gagacgtcca
     3541 aacgcccggg tattcacctt cagcacctcg gttctgtcca ataaatccgt aataagcgtc
     3601 gtcctgctaa agagggccat gggggttgtt ggagtaaagt aatagttcgt tatcctgtcg
     3661 ctaaagatac gcgaagccct gccggaggct aaatccggcg caaaaaatac ccagaaacca
     3721 ttgctggctg gacagtgaaa cggaaaaatg tgactacatg ctgctcaaac gatggtcggc
     3781 tcactgatgg gccgctccga cgcgacggct gcgagcgccg ctcccacgca aaggcgttcg
     3841 aaagcccgca gcgggattcg tctttctgaa ccttaccgcc gtccatacat ggtccagcgg
     3901 tacatcacgg actacttcgt accccgcacc ggttatacag ccccagctgc gttcccggtt
     3961 aagcgtggtg acgatcttgg agccgggctt cggataggca acccagaact ttccctccgg
     4021 ccggagggcg ggcagcactt cctgcacgat catccccagc tgctgctcgt tgatagcgaa
     4081 gacgagcgca aaatcgatct tcttgctttt cagcaatggg gtgagattct tggcaaaggc
     4141 cagcttgacg aattgtttct cgatggagga aggaagaccc tggatcaaca ggttccgctc
     4201 atcggtcaat tgtaatttct ccagtaaagt ttgagacatt ctggaccggt ttagtttaaa
     4261 agacagaccg caaagctacc gagcgatgcg gggttgcaaa aataagaaat tattctttgt
     4321 cccggtcggt tacatgtctc cgctgacgcc gccggtcacg atatctaagg atgggtacct
     4381 tgttctcgct tcctctgagc agacgagaga tatttttctg atgggtaagg actaccagta
     4441 aggcgacggc gatggcaaaa ccccgataaa gggtttcctt cgcgtcgaag atatacagga
     4501 taaagaccgc aaaagctacg ctggcaagga tcgaactcag cgagacaaat cgggtgaggt
     4561 ataagaccag caaaaagacc gctacgcagc acactgcagc cagcggctga atggcgagga
     4621 tcataccgaa cagggtggct acacccttcc cgccgcggaa ctccgcccag atcggaaaga
     4681 tatgaccggt gacggccgcc agacccagac cgaccatgaa attggtccgt tcggcttcat
     4741 cgtgtaaata gtaaggaatg aggatataca ggctggtggc gatgactccc ttgataacgt
     4801 ctacggacat cactatagcg ccccacttcg aacccagcac ccggaaggta tttgtagctc
     4861 ccgcattgcc gctgccgtac tccctgatgt ctattccaaa aaacgctcga ctgacccata
     4921 ctgcggtagg aatagaacct atcaggtatg ccgatagaat tagcaaaacc tccttcatcg
     4981 aatggtcatt ggttttagtt ggacaacaaa tatacgaata aaatgaactc ccagcccttc
     5041 agcctcagct acttatttca ttttttcgga tttcagcaag atccagttct ctttcgtcat
     5101 ccgtaaggat attgaaaaat tagttttagc agcttcattt tcaatgtctt ccgagtcttc
     5161 ctttaacaag ccgctcagca gtataacgcc ccctgttgtc aattgttgct ccatcgcgga
     5221 aagctcttct aaaattacat gtttgtttat attggccagg atgatgtcga aaggcccccc
     5281 gtgcacaacc tccccttttt ggatattaat ccgtctacac ccgttggcct ccgcattttc
     5341 gcgggcattg tcgatactcc aatcgtcgca atcgattgct tccacgccgg ccgctcccag
     5401 cttttcggcg aggatggcaa gaactccggt gccggtcccg aaatcgagga cccgcttgcc
     5461 gtcgagcggc agtccctgca tggcctgcaa catcattaag gtcgtcgcgt ggtggccggt
     5521 gccgaaactc atcttgggcg tgatcaccag ttcgtacgca acgccgggga tggggtcatg
     5581 gaaatgcgcg cggacggcgc agaaaccttc gatcaccacg ggctgaaagc tcttctccca
     5641 ctcctcgttc caattctttt cggctatacg cgtgatctcg tatcccaacc ccaacgattt
     5701 caaacggtgc tcatcgaatc cggtctccga cacatacgcc atcaacagcc cttcctgctg
     5761 ttcaaacccg tcatatccca tctctgccaa cagtgcgatg agcacctcct gcaattctgc
     5821 gttcgccttt atcgtgatcc ctatatgcat aaaaaaaggc cgcagcgcgg ccttattaaa
     5881 agtcatttga ttcttattcc tgatcctcgt gaccgccggc gggcgatttg gcatgaccgt
     5941 gtccgccgcc atgacccgaa ccggatcctt caggtttcgg catcagcacc ttgcggctga
     6001 gcttgaactt gccggtcttt tgatccagac cgatcagctt caccttcacg gtatcgcctt
     6061 ctttcaggac gccgtccatt gtctccagcc gtttgtgcga gatctcgctg atgtgcagca
     6121 agccttgctt tccgggcagg aactcgacaa acgcgccata tggcatgacg gacttgacct
     6181 tcgcctcgta aacggagcca acctcgggtt gcgccacgat gctcttgatc caggtgactg
     6241 ccttgtccag gccttccttg gcgggagaga aaatgctcac ttcgccaaaa ttgccgactt
     6301 cttccaggtt gatcgtagta cccgtctcgc gctggatctc ctggatgatc ttgccctgag
     6361 gtccgataac ggcgccgatg aactccttgt cgataaagat cttcaccata cgcggtgcat
     6421 gcggtttgac atcggcatgt gcagcgggga tacattcgta catggcgtcg agaatatgca
     6481 gccttccctt acgggcctgc tccagcgcct cgcgcatcgt attcatgctc agaccgtcga
     6541 ccttgatatc catctgaaca ccgcagatgc cttcgcgcgt cccggtcacc ttgaagtcca
     6601 tatcgccgag atgatcttcg tcaccgagga tatccgtgag gatggcattt ttgttgtctg
     6661 caggacgcgt gatcagaccc atagccacgc cgctgacgtg tttaggtatg ggaacacccg
     6721 catccatgag cgccaggctg cccgcgcaaa ccgtagccat cgaagaggaa ccgttggatt
     6781 ccaggatatc gctgacgacg cgtacggtat aaggataatc gcttcccggc atcatttgac
     6841 gcagactgcg catggccagg ttgccatgac ctacttcgcg gcgactttgt ccccgcatca
     6901 tcttcacttc gccggtgctg aacggaggga aattatagtg caggaaaaat ttcgaatcga
     6961 cggattttgc ggcgctttcc accagcaact catcgagcgg agtgcccagg gtgacggtgg
     7021 tcagcgattg cgtttcgccg cgggtgaaca gtgcggaacc gtgcggggtg ggcagcggat
     7081 cgatctccat cgccagaggc cttacctggt ccagctgacg tccgtcgaga cggatgcggt
     7141 cgtcgaggat catgttccgg acgagctccc actggagatc ggcatagtat ttttttgcga
     7201 gcttcttgtc ggcgtcggtg acctcttcgc ccaatgaagc gatgagatct tccttcaatt
     7261 tatcaaaggc gtcgccgcgc tcgtgcttgc tgctgccggc acggcttatc tggttaatgc
     7321 ggtctgtcgt gaaggcggta accttttgct gaagctcgtc attctgcacg ggcctggtat
     7381 actcgcgttt gcccgctacc ccggccttgg acctcagctc atcctgggcg cggatatgaa
     7441 cgcggatggc atcgtgcgcc atctccagcg ccctgaccag gtcttcctcg ctgcattcct
     7501 tcgcttcgcc ttcgaccatc atcaggttct tatcggttgc ggcgatcatg aattccatat
     7561 cggc
//
LOCUS       DASYSV010000490         6810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1897619, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000490 DASYSV010000000
VERSION     DASYSV010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897619"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /gene="nrfD"
                     /locus_tag="VGQ51_15105"
     CDS             complement(<1..1008)
                     /gene="nrfD"
                     /locus_tag="VGQ51_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEV9037962.1"
                     /translation="MSSLRYESQTRAPLVDGTKDYHQVTEDIIRPVEAPPSRLWWTGF
                     LISVACLIFGIWSVTEQVIYGVGQWNLHRTVGWGWDITNFVWWVGIGHAGTLISAILL
                     LFRQGWRTGVNRAAEAMTIFAVMCAGQFPIWHMGRVWLCFFIMPYPNTRGPVWPNFNS
                     PLLWDVFAISTYFTVSVLFWYSGLIPDIATVRDRAKLKWRKFMYGVLSFGWAGGTKAW
                     QRQEALALVLAGLSTPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAMV
                     QTLLVVSRKVLKLEEYITLEHIEAMNKVIVLTGSIVGIAYLSELFISWYSQNKFEDWA
                     FA"
     gene            complement(1051..4242)
                     /locus_tag="VGQ51_15110"
     CDS             complement(1051..4242)
                     /locus_tag="VGQ51_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEV9037963.1"
                     /translation="MSDKKYWQSFGERNESEAFQKSSEEEFKESLPLEGLDGKGLFDA
                     KHPRRDFLKYLGFSTAAATLAASCEMPVRHVVPYLNKPENMIPGVADYYATTYTLGGD
                     VIPLVAKVRDGRPIKLEGNTLSSRTIGGTSARVQASVLDLYDMARLRYPVQIVSGKAQ
                     EVSTFEAFDKIAADALESAGGPIVLLTGTMTSPTTKQIVTDFLAKYPSSRHVQYDADS
                     YSGVLLAAEASYGKRMIPSYRFDKAEVIVSLGADFLGTWLSPVEFARHYSKGRKIDES
                     NPSMSKHYQFESLLSMTGGSADERYTHKPSQSGAVALALLAELGGSVTAPALPDNVKA
                     GIKRAARDLQDNKGKGLVISGSNDVHVQTIVNAINEAIGASGSTVDWSSPLQTRQGID
                     GDMNSLIADLNDGKVGALLIYGVNPAYDYYDAEKFKNGLKKVKSSISFNDRLDETTEL
                     CKLVLPAPHFLESWGDTEAKPGFYSFLQPTIAPLFKTRPFEDSLLKWTGNNTTYEAYF
                     KQYWVGKLGSIEAYEKALQDGVVEPATAAAAGGGAYSNAKVADAATAIAAIKAAGPLE
                     IVFYQSIAMGVGAQANNPWLLELPDPVTRISWDNYAILSPKLAKDKFNIDVSDRRQAD
                     QYEVYMDKQIITLKLGGREPMNIPAIIIPGTHNEVLGIAVGFGRKSANPDDTVHNIGK
                     AAAGVGFNAFPMRAFNGTTVDWYTAGADFDKTNETFKLAQMQTHGTYEGRVEVVKDIT
                     LDKFKQDPQYVVDQREEELKPFGGIEHFEEQGTLYDVYDKPGIHWGMSIDLNSCIGCA
                     ACVVACHAENNVSVVGKNEVLRFHDMHWIRIDRYYSMPPGSKFDNPEEVQVVFQPMLC
                     QHCDNAPCENVCPVNATNHSSEGLNQMAYNRCIGTRYCENNCPYKVRRFNWADYNGSD
                     SFPNNQDQKVVGVLDPAVFQMNDELTRMVLNPDVVTRGRGVMEKCSFCVQRLQDGKLK
                     AKKENRTLEDMVDVQTACQQACPTEAIVFGNANDRTSRLSTTRKGNSKRLYHVIEELH
                     TLPNISYLAKVRHSEAAFGAEGPEEETRIEKQS"
     gene            complement(4298..5560)
                     /locus_tag="VGQ51_15115"
     CDS             complement(4298..5560)
                     /locus_tag="VGQ51_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV9037964.1"
                     /translation="MITHRSTVKKLIFSILPLALVLLANTTFAQDGKALFQANCASCH
                     NPFKVITGPALHGVTERVTDKKLLHDWIHNNQKVLASGNKYFNNLYTEYSKTNMSLFP
                     DLKDNEIDAILNYVETAKPPSATPEPGAPTAQAPEGDSTLLYGILTLILAVIMMVLLQ
                     VNSSLRKMADDKEGTPSSEPIPFYRNKVYIAGAVLILFVLGGYWVVQGAIGLGRQQGY
                     QPIQPIFYSHRVHAGINQISCLYCHSNAMDSKHATVPPLNVCMNCHAAINDYSHGPKL
                     YREDGTEVDGTQEIQKLYSYTGYDPKTQKYNTENAKPVEWIKIHNLPDHVFFSHAQHT
                     KAGQVQCQTCHGPIQEMDEVKQFAPLSMGWCINCHRTTKVNFSDSSGNGNKFYNIYER
                     FHKDLKTGQMDSVTVENIGGTECQKCHY"
     gene            5796..6374
                     /locus_tag="VGQ51_15120"
     CDS             5796..6374
                     /locus_tag="VGQ51_15120"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV9037965.1"
                     /translation="MKQLAIFASGTGSNAARIIDYFRHHREIKVGLIVCNKPGAGVLN
                     VAEREGLPVLLIEKERFFRGDGYVEDLRRREIGLIVLAGFLWKIPLTLVRAYPGKIVN
                     IHPALLPKYGGKGMYGRFVHEAVLAASEKETGITIHYVDELYDHGAPIFQAKIVVEPG
                     DTPDTLAKKIHLLEYEHFPRIIEEVLHLQNHR"
     gene            6551..>6810
                     /locus_tag="VGQ51_15125"
     CDS             6551..>6810
                     /locus_tag="VGQ51_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037966.1"
                     /translation="MYRTRPLEAVSVMSTGGRGTTTDSSGNYYITVTTKDSIWFSYLG
                     RTTVKFPVGSINYFSGFDIALHVDPTELKEVRVMPRDYKADS"
BASE COUNT         1477 a         1727 c         1931 g         1675 t
ORIGIN      
        1 agcgaatgcc cagtcttcga atttattctg gctataccag gagatgaaca gctcggacag
       61 ataggcgata cccacgatcg aacccgtgag cacgatcacc ttgttcatgg cctcgatatg
      121 ttcgagcgtg atatattcct caagcttcag cactttgcgg ctgacgacga gcagggtctg
      181 aaccatcgcg aaacccgaga agatggcacc cgcaacgaag tagggaggga agatggtcgt
      241 atgccagccc gggatgaccg aggtagcgaa gtcgaaggac acgatcgtat gtacggaaag
      301 caccagcggc gtactcagac cggccagcac gagtgccagc gcttcctgcc gttgccaggc
      361 tttggtacca ccggcccaac caaatgaaag aacgccgtac atgaacttgc gccatttgag
      421 cttggccctg tcgcgaacgg tggcaatatc ggggatcaga cccgagtacc agaacagaac
      481 cgataccgtg aaatatgtgg agatcgcgaa tacgtcccaa agcagcgggg agttaaagtt
      541 gggccatacg ggaccgcggg tattgggata aggcatgatg aagaaacaca gccagacacg
      601 acccatgtgc cagatcggga actgaccggc gcacatcacc gcgaagatcg tcatggcctc
      661 agcggccctg ttcacacccg tacgccaacc ctggcggaac agcaaaagga tcgcagagat
      721 cagggttccg gcgtgaccga taccgaccca ccagacgaag ttggtgatat cccagcccca
      781 gcccacggtc cgatgcaggt tccactgccc gaccccgtag atcacctgtt cggtgaccga
      841 ccagatgccg aagatcaaac aggcgacgct gatcaggaaa cccgtccacc atagtcggct
      901 gggcggcgct tcgacgggtc ggatgatatc ttccgtcacc tggtggtaat ccttggttcc
      961 atctaccagc ggggctcttg tctgtgattc atatcgtaat gatgacatag tctattgagc
     1021 tttttgctaa tacttttgaa ttagattttc ttacgactgt ttttctatcc tcgtctcttc
     1081 ctccggtccc tcggcgccga atgcagcctc cgaatgacgc accttggcca ggtagctgat
     1141 gttgggcagg gtatggagct cttcgatcac atggtacagt cgtttggaat tgcccttgcg
     1201 ggtcgtgctg agacggctgg tccggtcatt ggcattgccg aatacgatgg cttcggtcgg
     1261 acaggcctgc tggcaggcgg tctgaacgtc gaccatgtcc tcgagagtac ggttctcctt
     1321 tttggccttc agcttaccat cctgcaaacg ttgtacgcag aaggagcatt tttccatgac
     1381 gccgcggccg cgggtaacca catccgggtt cagcaccata cgggtcagct cgtcattcat
     1441 ctgaaaaact gcggggtcga gtacgcccac caccttttgg tcctggttgt tggggaagct
     1501 gtcagagcca ttatagtcgg cccagttgaa acgtctcact ttatacggac agttgttctc
     1561 acaataacgg gtgccgatac aacggttata agccatttga ttcagtcctt cggagctatg
     1621 gttggtagca ttgacggggc aaacgttctc gcatggagcg ttgtcgcaat gctgacaaag
     1681 catcggctgg aataccacct ggacctcttc cgggttgtcg aatttgcttc ccggaggcat
     1741 cgaataatat cggtcgatcc tgatccagtg catgtcgtgg aaacgcaaga cctcattttt
     1801 gccgaccacg gacacgttgt tctcagcatg acaagccact acgcaagccg cgcagcctat
     1861 acaagagttg aggtcgatgg acatacccca gtgtatgccg ggtttatcat agacgtcgta
     1921 aagcgttccc tgctcttcga aatgctcgat accgccgaaa ggtttcaatt cttcctcgcg
     1981 ctgatcgacc acatattgag ggtcctgttt gaatttgtcg agcgtgatat ccttcactac
     2041 ctccacgcgg ccttcatagg tgccatgagt ctgcatctgc gccagcttga aggtttcgtt
     2101 cgtcttgtcg aaatcggcgc cggcagtata ccagtctacg gtcgtaccat tgaaagcccg
     2161 catcgggaag gcattgaagc ctacgccggc ggctgccttt ccgatattat gcaccgtatc
     2221 gtcgggattg gcgcttttgc ggccgaaacc gacggctatc ccaaggacct cgttatgggt
     2281 gccagggatg atgatcgcgg ggatattcat cggttccctt ccgccaagct taagcgtgat
     2341 gatctgtttg tccatatata cctcgtactg gtcggcttga cggcgatccg atacgtcgat
     2401 attgaacttg tctttagcca gcttgggaga gaggatggcg tagttgtccc aggagatccg
     2461 ggtgacggga tcgggcagtt cgagcagcca ggggttgttg gcttgtgcgc cgactcccat
     2521 agcgatgctt tgatagaaaa cgatttccag cggtcccgcc gccttgatcg ccgcgatggc
     2581 agtggccgca tccgcaacct tggcgttgga gtaggcgcct ccgccggcgg cagcggccgt
     2641 agccggctcg accaccccgt cctgtagcgc tttttcgtag gcttcgatgc ttccgagttt
     2701 tccgacccaa tattgtttga agtaggcttc gtaagtggtg ttgttgccgg tccatttcag
     2761 cagcgaatct tcaaagggcc gcgttttgaa taacggggcg atcgtcggct gcaggaagga
     2821 ataaaatcca ggttttgctt cggtgtctcc ccagctttcg aggaaatgtg gagcgggcag
     2881 gaccagctta cacagttcgg tcgtttcgtc gagacggtcg ttgaaggaga tggaagactt
     2941 caccttcttc aggccattct tgaatttctc tgcgtcataa taatcatacg cgggattgac
     3001 gccatagatc agcagggcgc cgacttttcc gtcattcaga tcggcgatca ggctattcat
     3061 atcgccgtcg atgccctgac gcgtctgcag gggagacgac cagtcgacgg tcgacccgga
     3121 agcgccgatg gcctcgttga tggcattgac gatggtctgg acatggacgt cattgctacc
     3181 gctgatgacc aagcctttgc ctttattgtc ctggaggtcc ctggcggccc gtttgatacc
     3241 ggctttcaca ttgtcgggca gggcgggggc tgtaacggaa cctcccagct cggccagcag
     3301 ggccagtgcg acggcgcccg attggctggg tttatgcgta taccgttcgt ctgcgctgcc
     3361 tcctgtcatg gagagcaggc tttcgaactg gtaatgtttg ctcatcgacg gattgctctc
     3421 gtcaatcttg cggcctttcg aatagtggcg ggcgaactcc acggggctga gccaggtgcc
     3481 tagaaaatca gcgcccaggc tgacgatcac ctccgcttta tcaaagcgat aggaagggat
     3541 catccttttc ccatagctgg cttccgctgc gaggaggaca ccgctataag aatcggcgtc
     3601 atattgtacg tggcggctgc tgggatattt ggcgaggaaa tcggtgacga tctgtttggt
     3661 ggtgggcgag gtcatcgtgc cggtcagcag gacgatcgga ccgcccgcgg attccagggc
     3721 atcggccgcg attttatcga aagcttcgaa cgtacttacc tcctgcgcct ttccactcac
     3781 gatctggacg gggtagcgca gccgcgccat atcatagagg tcgaggaccg acgcctggac
     3841 gcgggccgag gtgccaccaa tagtgcgcga cgacagcgta ttgccttcca gcttgatcgg
     3901 gcgaccgtcg cggaccttgg ccacgagggg aataacatcc ccgccgaggg tataagtggt
     3961 cgcgtagtag tcggcgacgc cggggatcat gttctccggt ttgttgagat aaggcactac
     4021 atgccgtaca ggcatctcgc agctcgcggc cagcgttgcg gcagccgtgc tgaagccgag
     4081 atatttcaaa aaatccctgc gggggtgctt ggcatcgaac aggccctttc catccaggcc
     4141 ttcgagggga aggctttcct taaactcttc ttcgcttgat ttctgaaacg cttcactttc
     4201 attgcgctct ccgaaacttt gccaatattt tttgtcgctc atattgtaga gaaattaaac
     4261 ttttatgtga tccgcgcttg cggagggagg gattagatta atagtgacat ttctggcact
     4321 cggtaccacc gatgttctcg acggtgacgg aatccatttg gccagtcttc aggtccttgt
     4381 gaaacctttc gtaaatgttg taaaacttgt tcccgttgcc gctgctgtcg gagaaattga
     4441 ccttggtggt acgatggcaa ttaatgcacc atcccatact gaggggggcg aattgtttca
     4501 cctcgtccat ttcctggatc ggcccgtggc aggtctggca ttgcacttgt ccggccttgg
     4561 tatgctgtgc gtggctgaag aatacgtggt cgggaaggtt atggatcttg atccattcta
     4621 ccggtttggc gttctcggta ttatactttt gggtcttggg atcgtacccg gtgtaggagt
     4681 atagtttctg tatctcctgc gtaccgtcca cttcggtgcc gtcctcgcga tacagcttgg
     4741 ggccgtggct atagtcattg atcgcggcgt gacagttcat acagacgttc agcggcggta
     4801 ccgtagcgtg tttgctgtcc atcgcattgc tatggcaata aagacagctg atctggttga
     4861 tcccggcgtg aacgcggtgc gaatagaaga tcggttgtat cggctgatag ccttgctggc
     4921 gacccagtcc gatggcgccc tggacgaccc agtatccgcc gaggacgaag aggatcagca
     4981 cggcgcctgc gatatatact ttattacgat agaaggggat aggctcggag gaaggagtgc
     5041 cctctttgtc gtcggccatc tttctgaggc tgctgttcac ctgtaacagc accatcatga
     5101 tcacggcgag gatgagggtc aggataccat ataatagggt gctgtcgcct tcgggagcct
     5161 gcgcggtcgg agcgcctggc tcgggagtgg cggacggcgg tttggccgtt tccacatagt
     5221 taaggatggc gtcgatctca ttgtctttga gatcggggaa caggctcatg ttcgtcttac
     5281 tgtactcggt gtacaggtta ttgaaatact tgttccctga agcaagcacc ttctggttgt
     5341 tgtggatcca gtcgtgaagg agctttttat ccgtcacccg ttcggttaca ccatgtaatg
     5401 caggacccgt aatcaccttg aaagggttat gacaggaagc acaatttgcc tgaaacaaag
     5461 ccttaccatc ctgcgcaaaa gttgtattag ctagtaggac cagagcgagg gggaggatac
     5521 tgaatattag ctttttaact gtcgatctat gagtgatcat atatacaata tgttgtatag
     5581 atgtggaaaa atctccttaa caggttgcaa aaataagtca ggattttaac aatctatcaa
     5641 catgggaaaa tttttttttc ccttgctggg cgtgggtttc aacgaattac gacaaatcgc
     5701 gctgacgctc tgtcatagga ttgtcagcaa gaaatgcgcc cttaaagcat ggaaaatccg
     5761 acacgtatat aggattacaa ccgtagtttt gacatatgaa acagctggcc attttcgctt
     5821 cgggaacggg gtccaatgcg gcaaggatca tcgattattt tcgtcatcat agggagatca
     5881 aagtgggttt gatcgtttgt aataaaccgg gggcgggggt attgaacgtc gccgaacggg
     5941 agggtttgcc tgtgctgctg atcgaaaaag agcgcttttt caggggagac ggttatgtgg
     6001 aggatttgcg gcgacgggaa attggtctga tcgttctggc aggattctta tggaagatcc
     6061 ccttgacgct ggtccgggcc tatccgggaa agatcgtgaa tatccatccg gcattgctcc
     6121 cgaaatatgg agggaaaggg atgtatggca ggttcgtcca cgaggccgtg ctggccgcct
     6181 cggagaagga gacgggtata accattcatt acgtcgacga gctatatgat cacggagctc
     6241 ctatctttca ggcgaagatc gtcgtggaac cgggcgatac cccggatacg ctcgcaaaaa
     6301 aaattcattt actggaatat gagcatttcc ccaggatcat cgaggaggtg ttgcatctgc
     6361 aaaatcatcg ttaaaaatct atcgctcccg gccgttatgt ccggttttgg tagtaatatc
     6421 acgacaaaat aagcactgat tgacctgttc tacccataag ttcttactgc cggtgttttc
     6481 ccttttctct ctccttctat gcggcggggt cagggggcag caggtcacaa tccacggcac
     6541 tgtatataat atgtatagga ccaggccgct ggaggcggtg agcgtcatga gcaccggcgg
     6601 gaggggtacc accacggatt caagtggtaa ttattatatc acggtgacga cgaaggattc
     6661 catctggttc tcctacctgg gccggacgac ggtaaagttc ccggtgggaa gcatcaatta
     6721 tttttccggc tttgatatcg cgttgcacgt ggaccccacc gaattgaaag aggtcagggt
     6781 catgccgcgg gactacaagg ccgattcgat
//
LOCUS       DASYSV010000491         5658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1632126, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000491 DASYSV010000000
VERSION     DASYSV010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632126"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VGQ51_15130"
     CDS             <1..660
                     /locus_tag="VGQ51_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037967.1"
                     /translation="VLDVRAAYDTALSIYHSYLTPESALFRGDEYATYDFRLREGNPY
                     FGDKRRHPGTVVYDSVMYNHVLLLYDEVKDLVILFDFTNLFKITLFPELIDRFTIEDH
                     HFIRLKDSLNPNQPRNGFYEVMYQGRMTLLKKEKKTVQEDLNAGTVAEYYIQGADTSY
                     YLKKNNVYYPVKNTKSLLHALKDRSKDVKRFIRSNHLSMRRDREHTLLKVAAWYDSSS
                     Q"
     gene            641..3439
                     /locus_tag="VGQ51_15135"
     CDS             641..3439
                     /locus_tag="VGQ51_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9037968.1"
                     /translation="MILLLNKYGRWAGVLFVLYLVMPMPTRAQQTTRDRVTLDLHKAS
                     MDTVLLLVQQQTGIRLYYDTTDLDTTRLDIKVDQRPVSNVLTDIFQGTDLSFSTDRYG
                     NIFITKGAAIETELPGDYFHARTAGNAAVAAAGDSIRDYLENVGEKPVTTLENKLFTI
                     GEKISGPLHGIVNVAGYARDARTGEPIAGASIYVENPRIGVFSDQYGYYSISLPRGRH
                     ILNIQSIGMHDTRRLIMVYGDGKLNIDLQTQVLTLKKVIVSAEKVSNVKRTEMGVQKL
                     DIKTIKQVPVVFGEADVMRVITTLPGVKTVGEASTGLNVRGGSSDQNLVLFNEATIYN
                     TAHFFGMFSVFNPEVVKDVELYKSSIPARFGGRLSSVLDISSREGNKKDFTGSAGIGL
                     LTSRLNIEGPIVKDKMTFILGGRTTYANWLLQDLPAQYKNSRASFSDLNLQVTAEPNK
                     KNTLYFTGYLSEDHFNLNNDTTYGYGNRNLSLKWKHIYNNRLYSTAATGYDRYQYSIS
                     STYNRVNAYKLGFDINQLYFRLNFNYFLSSSHTLDFGLSTLRYKLHPGSYIPIGEKSL
                     VVPDTLQTEQAQESALYLSDRFTITNALSLEAGVRYSLFNDLGPSTVNNYAAGQPVTT
                     DNFLGTTSYPTGKKIVNYGGPEYRISARLALTDNLSLKAGYNTQQQFIHMLSNTTAEA
                     PTDIWKLSDPHIKPQSGDQYSLGAYRNFKNNTIETSLEVYYRNIDNYLDYRSGAQLIM
                     NHHIETDVLGTKGKAYGVELLIKKLTGKLNGWFSATWSRTLLQANDSAQGIVNNGNWY
                     PADYDKPVDVTAVGNYRFTHRFSISLNATFSTGRPITLPIGVYNYAGSLRALYSDRNA
                     YRIPDYFRTDFSMNIDGNHKVHQKTHNSWTIGVYNLTGRHNPYSVYYTSENGVVNGYK
                     LSIFGSAIPFINYNIRFN"
     gene            3445..4587
                     /locus_tag="VGQ51_15140"
     CDS             3445..4587
                     /locus_tag="VGQ51_15140"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEV9037969.1"
                     /translation="MRRRLHIVLALFTVFLLWRCKSSYISPYKAPPTGYLVVEGYISG
                     NTPTRFVLSRTVKLPGDSLPPPETGASVQVEGSDNSVRPLPEVSKGVYRDSFALPAQA
                     QYRLRIQTTGGETYLSDYVPFKNTPSIDSVNFTAGPDGINIYVNSHDPSNATRYYQWQ
                     WDQTYEYHSGASSMFEYDRKHDTVIERPDSEQIWRCWLASSSTSILVSTSTRLAQDVI
                     SLYPLKSIPIDNVQLSVLYTILVRQYALTEAGYDFLSLMQKNTESLGTIFDAQPSQIT
                     GNIHSLTHPGEQVIGYVSAGTVQQQRLWISPGQVPGYYYFFCDQADTLLPSGTDLQKN
                     FGGMYTPIYPKALGPIITGYYSNWTDCIDCRIVQGGTTKKPSFWPN"
     gene            4598..>5658
                     /locus_tag="VGQ51_15145"
     CDS             4598..>5658
                     /locus_tag="VGQ51_15145"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEV9037970.1"
                     /translation="MTLRNIILTGIALIASMGCKDEFISPYKAPPTGYLVVEGYISGN
                     TRTRFALSRTVSLPGDSTPPPEKGAKVQVEGSDNSIWPLPEGSAGVYSDSFTLNPQLQ
                     YRLRIRTSKGETYLSDFAPFKITPPIDSISWTNGSNGVVIYANTHDPANATRYYQWNW
                     DQAYEYASGEYSEYEYNRSNGGVFFRPDSDQINHCWKYASSTSILVTTSTKLAQDVIS
                     LYPIKMIPPNDIQLSILYSILVRQYSLTMDGYNFLTLMQKNTESLGSIFDAQPSQITG
                     NIHSLSHPTEQVIGWVSAGTVTSQRIYIGRADVPSNYVFGCEFIDTLLPADTDLRKNF
                     TVEFDPVYGDFVAGRLIGY"
BASE COUNT         1404 a         1663 c         1376 g         1215 t
ORIGIN      
        1 gtcctcgatg tccgcgcggc ctatgacact gcactcagca tctatcattc atacctcacc
       61 ccggagtccg ctctgttccg cggtgacgaa tatgccacct acgattttcg cctgcgcgaa
      121 ggcaatccgt atttcggcga caaaagacgg catccgggca ccgtcgtcta tgacagcgtc
      181 atgtataacc acgtcctcct gctctacgac gaggtaaaag accttgttat cctgttcgat
      241 ttcaccaatc tttttaagat cacccttttc cccgaattga tcgaccggtt cacgatcgaa
      301 gatcatcact tcatccggct gaaggacagt ctcaatccca atcagccccg caacggcttt
      361 tatgaggtga tgtaccaggg caggatgaca ctactgaaaa aggaaaagaa gaccgtccag
      421 gaagacctca atgccggcac agtggcggaa tattatatcc agggggccga cacgtcctat
      481 tacctgaaga aaaacaatgt ttactatcct gtaaaaaata ccaaatccct gctgcatgcg
      541 ctgaaggacc gaagcaagga tgtaaagcga ttcatccgta gtaaccatct gtccatgcgc
      601 agggacaggg aacatacact gttaaaagta gccgcctggt atgattcttc ttctcaataa
      661 gtacggtcga tgggccggcg tcctgttcgt gctctacctg gtaatgccca tgccaacccg
      721 ggcccagcaa actacccgcg accgggtcac gctcgatctg cataaggctt ccatggatac
      781 cgtgctcctg ctggtgcagc aacagacagg catacgcctt tattacgata cgacggatct
      841 cgataccacc aggctcgaca tcaaagtgga tcaacgcccc gtgagcaatg tgctgacgga
      901 tatcttccag gggacggatc tcagcttttc gaccgaccgg tacgggaata tctttatcac
      961 caaaggtgcg gccatcgaga ccgagctgcc aggcgattat tttcatgcgc ggactgcggg
     1021 caacgccgcc gtggccgcag ccggcgattc gatccgcgat tacctggaaa acgtcgggga
     1081 aaaacccgtc accacactcg agaataaact cttcacaatc ggtgaaaaga tctccggccc
     1141 gctgcacgga atagtcaatg tcgcgggcta cgcccgcgac gcccggacgg gcgaacccat
     1201 cgcaggcgca tcgatctatg tcgaaaaccc gcggatcggc gtcttctccg atcaatacgg
     1261 ctattattcg atctcgcttc cccgcggccg ccatatcctc aatatccaaa gcatcggcat
     1321 gcacgatacg cggaggctga tcatggtcta cggcgacggc aagttgaaca tcgatctgca
     1381 aacgcaggtg ctgacgctga agaaagtgat cgtttcggca gagaaggtca gcaatgtcaa
     1441 acgcacggaa atgggcgtac aaaagctgga tataaagacg atcaaacagg tgcccgtcgt
     1501 attcggagaa gccgatgtca tgcgcgtgat cacgaccctg ccgggcgtta agacggtggg
     1561 agaagccagt accggtctca acgtgcgggg cggctcctcg gatcagaacc tcgtcctgtt
     1621 caatgaggcg acgatctaca ataccgcaca cttttttggt atgttctccg tcttcaatcc
     1681 cgaggtcgta aaagacgtcg agctgtacaa gagcagtatc cccgcccgtt ttggcggccg
     1741 tctttcctcg gtgctggaca tcagcagccg ggaaggcaat aaaaaagact tcaccgggtc
     1801 ggccggtatt ggcctgctga ccagccgcct caatatcgaa gggccgatcg tcaaggataa
     1861 aatgaccttt atcctcggag gacgcactac ttatgccaac tggctgttgc aggatcttcc
     1921 ggcccagtac aagaacagcc gcgcttcctt ttccgacctc aatctccagg tgaccgccga
     1981 gcccaacaaa aagaataccc tgtatttcac cggctacctg agcgaagacc atttcaatct
     2041 caataacgat accacttacg gctacggcaa ccgcaacctc tcgctgaaat ggaaacatat
     2101 ttacaacaac cggttgtaca gcacggccgc tacaggctat gaccgctatc agtactcgat
     2161 atcaagtaca tacaatcgtg tgaatgccta taagctgggc ttcgatatca accagctcta
     2221 tttccggctg aacttcaact atttcctgag cagcagtcat acactcgatt tcggactaag
     2281 caccctccgt tacaagcttc atcccggctc atatataccg ataggcgaga aatcgctggt
     2341 ggtacccgat accctgcaga ccgaacaggc acaggaaagc gcgctatatc tcagcgaccg
     2401 gtttacgatc accaacgccc tgtcgctgga ggcgggtgtc cgctattcgc tgttcaacga
     2461 cctcggtcct tctacggtga acaattatgc ggcgggacaa cccgtaacga ccgacaactt
     2521 cctgggcacg accagctatc ccaccggaaa aaagatagtc aactacggcg gacccgaata
     2581 ccggatctcg gcacggttgg ccctgacgga caatctttcg ctgaaggcgg gctataacac
     2641 gcagcagcaa ttcatccaca tgctgtcgaa cacgacggcc gaagccccta ccgatatctg
     2701 gaaactaagc gatccccata tcaaaccgca atcgggcgac caatattcgc tgggcgctta
     2761 caggaatttc aagaacaata cgatcgagac ttcgctggaa gtgtattaca ggaatatcga
     2821 caattacctc gattaccgga gcggggccca gctgatcatg aaccatcaca tcgagactga
     2881 tgtgctcggg acgaaaggca aggcctatgg ggtcgagcta ttgatcaaaa agctcacggg
     2941 caagctcaat ggctggttca gcgccacctg gtcgcgaacg ctgctccagg ccaatgattc
     3001 ggctcagggt atcgtcaata acggcaactg gtatcccgcc gattatgaca aacccgtcga
     3061 cgtgacggct gtcggcaatt accggttcac acaccggttc agcatatcac taaatgccac
     3121 gttcagtacg ggcaggccga tcaccctccc catcggcgtg tataactatg cgggctcgct
     3181 gcgggcgcta tactcggaca ggaatgcgta ccggattccg gattatttcc gcaccgattt
     3241 ctccatgaac atagacggca atcataaggt gcaccagaag acgcataatt cctggaccat
     3301 cggggtatac aacctcaccg gcaggcataa cccttattcc gtgtactata cttccgagaa
     3361 cggggtcgtc aacggatata agctgtcgat cttcggcagc gccatcccct ttatcaatta
     3421 taacatcagg ttcaactagc gcatatgcga agacgtctcc atatcgtgct ggccctgttc
     3481 acggtgttcc tcctctggag atgcaagagc tcctatatca gtccctacaa ggcgccaccc
     3541 accggttatc tggtcgtgga aggatatatt tccggcaata cgcctacccg gtttgtcctc
     3601 agccggactg tcaaattgcc cggcgactcg ttacctccac ccgaaaccgg cgccagtgta
     3661 caggtagaag gcagcgacaa ttctgtccgg cccctgcccg aagtaagcaa aggcgtgtat
     3721 cgcgattcct ttgcgctgcc cgctcaggcc caataccggc tgcgtatcca aaccacgggc
     3781 ggtgagacgt atctttccga ctatgtaccg ttcaaaaaca cgccttccat cgatagcgtc
     3841 aatttcacgg cgggaccgga tggcatcaat atctatgtca attcgcatga tccgtcgaac
     3901 gcgacccgct actatcaatg gcaatgggac cagacctatg aataccactc gggtgccagt
     3961 tcgatgttcg aatacgaccg gaaacacgat accgtgatcg aacggcccga ttcagagcag
     4021 atctggcgat gctggctggc ttcgtcatcc acttcaattc tggtgagcac gtcgacgcgg
     4081 ctggctcagg acgtgatcag cctgtaccct ctcaaatcga tccccatcga caacgttcag
     4141 ctgagcgtgc tgtacaccat cctcgtcagg caatacgcac tgaccgaagc cggttatgat
     4201 tttttaagcc tgatgcaaaa aaataccgag tcgctgggta ccatcttcga cgcgcaaccg
     4261 tcgcagatca ccggcaatat ccacagcctg acacatcccg gcgaacaggt catcggctat
     4321 gtttcggcgg ggaccgttca gcaacagcgg ctttggatct cgccggggca ggtgccggga
     4381 tactattact ttttttgtga ccaggcggat accctcctgc catcgggtac cgatctgcaa
     4441 aaaaatttcg gcggcatgta tacgccgatc tatccaaagg ctttgggacc gatcatcacc
     4501 gggtactatt caaattggac cgactgtata gactgccgga tcgtgcaagg cggcacgact
     4561 aaaaaacctt ccttctggcc aaattaacaa tccatcgatg acgctacgaa acatcatatt
     4621 gacaggtatc gcgctgatcg cttccatggg atgcaaagac gaattcatca gcccatataa
     4681 ggctcctccg accggctatc tcgtcgttga aggttatatt tcggggaaca cgcgaaccag
     4741 gttcgcgtta agccggaccg tctctctacc gggcgattcg acgccgccgc cggaaaaggg
     4801 tgcaaaggta caggtggaag gcagcgataa ttcgatctgg ccgctccctg aaggatccgc
     4861 cggcgtctac agcgattcct ttacgctgaa cccccagctt caataccggc ttcggatccg
     4921 gacttccaaa ggggaaacct atctttccga ttttgcaccg ttcaagatca cgccgcccat
     4981 cgacagcatc agctggacca acggctctaa cggggtcgtc atctatgcca atacccacga
     5041 tcccgccaac gctacccgtt attaccaatg gaactgggac caggcctatg aatatgcctc
     5101 cggggagtat tccgagtacg aatacaatcg ctccaacggc ggcgtctttt tccgtcccga
     5161 ttcggaccag atcaatcact gctggaaata cgcctcttcc acttccatct tagtgaccac
     5221 gtcgaccaaa ctggcccagg acgtgatcag cctctaccct atcaagatga tcccgcccaa
     5281 cgacatccag ctaagcatac tgtacagtat cctggtgcga caatactcgc tgaccatgga
     5341 cggctataat tttttgaccc ttatgcaaaa gaataccgaa tcgctgggca gcatcttcga
     5401 tgcccaacct tcacagatca caggcaatat ccatagcctg tcccatccca ccgagcaggt
     5461 gatcggctgg gtctccgccg gcaccgtgac aagccagcgc atctatatcg ggcgggcgga
     5521 cgtaccttcc aactatgtat tcggttgcga gttcatcgat acgctccttc cggcggatac
     5581 cgatctgaga aagaatttca ccgtcgaatt cgaccccgtt tacggcgact ttgttgccgg
     5641 aaggctgatc ggttattg
//
LOCUS       DASYSV010000492         5615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1762851, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000492 DASYSV010000000
VERSION     DASYSV010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762851"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VGQ51_15150"
     CDS             complement(<1..1247)
                     /locus_tag="VGQ51_15150"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEV9037971.1"
                     /translation="MKINVAFVLLLLPVLAVAQSKPLPVYKNPSAPVESRVKDLLQRM
                     TLHEKIAQMQDLSFSDIYTGDSLDEEKLEKFLGGMSRGCMEGMGLTAEKYAGDVNRVQ
                     KYLVEKTRLGIPMLATSEALHGCVHGGATIYPQAIALGSTFNPSLINEMTRTITGELK
                     AQGIDQVLSPDLDLARELRWGRVEETYGEDPYLVGRMGVAYVKGFSENNIICTLKHFV
                     AHGSPRAGLNLASVAGGERELRSIYLPPFEAVIKEAHPLSVMNAYSAYDGIPMAASHH
                     VLTDILRHELGFKGYVYSDWAAVEMLYNFQHTARDAADAALQAVRAGLDMEIWSDCFE
                     KLDSLVTSGALPVRYIDSAVSRILRVKFSIGLFEHPYGDWAGGKKMHTPASVQLSLNI
                     ARESIVLLKNEGRLLPFRDNIKSL"
     gene            complement(1339..2427)
                     /locus_tag="VGQ51_15155"
     CDS             complement(1339..2427)
                     /locus_tag="VGQ51_15155"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEV9037972.1"
                     /translation="MNRPLIVDAHLDLAMNAIEWNRDLTRPLAEIRQREMNMKDKPDR
                     GKGTVCFPELRKGRVGLVVATQLARYTPPGSGFAGWNSPQQAWAMTQAQLCWYREMEK
                     LGEMVPITNIEQLDHHLALWDDGAVEDEQKPIGYILSLEGADSLVDISYLHAAHAYGL
                     RAMGLSHFGPGRYAPGTKLQGPLTAAGIQLLKEMEPLNLILDVTHLTDEGFLQAIDLY
                     QGPIWASHHNIRKIVPNQRQLADEQIRLLIQRGAVIGGMLDCWAMDQRFIDTVSDPWQ
                     LDIRLENLVDHWDHICQMAGNSNHIAFGSDLDGIFGTEQAPWDLNSIADLQKYQDILA
                     RRGYAKADIENIFYKNWISFLRRAWNEG"
     gene            complement(2439..3404)
                     /locus_tag="VGQ51_15160"
     CDS             complement(2439..3404)
                     /locus_tag="VGQ51_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV9037973.1"
                     /translation="METKYKGSPILLIANGRLGSSVTVCLLQAGHAVTLMTEDKEEAV
                     DGVNSHFARLSELNGKSLTPDMLEIEGFFDRKAAGHMIGIAITGENVNEKKARIQQLE
                     KLLFPGSIIAVNTESIPLSLLQDGCEHPERLIGLNWAEPAHTTFFLEIITNAATRQKL
                     VDDLYDLAKEHWNKDPYCVSKDVGIRAKMLSGMIREAFFLVQNGYATIEDIDRACRND
                     AGYYLPFAGNFRYMDLMGTYAYGLVMKDLNRELSKEAHIPRFFEEITDQGGLGMQNNK
                     GFYNYEPGDARRWNELFSLFSYQIQKIIEKNPFNGIQKPTGNYQV"
     gene            4066..4698
                     /locus_tag="VGQ51_15165"
     CDS             4066..4698
                     /locus_tag="VGQ51_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEV9037974.1"
                     /translation="MTFYQNIQETVTLHPSDKQLLQRMADSERQAFTLLYRRYWEELF
                     ITAARALRRKEEAADVVQDVFLSLWHRRNDLDVQGSLSAYLHTSVRYKCIHYVEKNIT
                     RRDYLIQFAESVRSSASKSAEINLQLKEIEQTVNKTVAKMPAKMQEVYKLSRQQHLSY
                     KEISDSMSISVETVKKHIHHALHLIRKDLLPYLFIIASLVFHFQWIFFSL"
     gene            complement(4674..5555)
                     /locus_tag="VGQ51_15170"
     CDS             complement(4674..5555)
                     /locus_tag="VGQ51_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9037975.1"
                     /translation="MRKKEGFQGQKAIVLPRNILSQYCERNKITNNLYVTDIGYYPKA
                     RFHYRERPRGAEQHILIYCQAGSGTAVIKKETYEIEAGDFFIVPMHTPHTYHANENNP
                     WTIYWIHFKGSISDSIIDLFYKQVNGYKGFIQYDRQSIALFDEIYGQLQRGYGIDNLI
                     YCNMCLGHFFTTVIYNDRYEPGIKIKNGDVMEKAIDFLSKNISEMLTLEAMASAVNLS
                     PSHFSFLFKARMGFPPMEYFNHLKVQKACQYLLFTDMRIREIALELGIEDAYYFARMF
                     TKIMGLSPKHYREKKIH"
BASE COUNT         1397 a         1429 c         1256 g         1533 t
ORIGIN      
        1 agcgacttta tgttatccct gaatggcagg agccggcctt cattctttag taagacgatc
       61 gactcccgcg ctatattcag ggatagttgt acggatgcgg gtgtatgcat ctttttccca
      121 cccgcccaat cgccataggg atgttcgaat aatccgatac tgaacttcac ccgcaggatc
      181 cttgacacag ccgagtcgat atagcgaacc ggcaacgccc cgctggtgac caggctatcc
      241 agcttttcaa aacaatcgct ccagatctcc atgtccagac ccgcccttac tgcctggaga
      301 gccgcgtccg cagcatccct ggccgtatgc tgaaagttgt aaagcatttc gaccgccgcc
      361 cagtccgagt atacataacc tttaaaaccc aactcgtgcc gaaggatatc cgtcagcaca
      421 tgatgcgatg ccgccatcgg gataccatcg taggcgctat acgcattcat gacactcagc
      481 gggtgtgcct ctttgatcac cgcctcgaac ggcggcagat aaatcgaccg gagttcccgc
      541 tctcctccgg cgacggaggc aagattcaat cccgccctcg ggcttccatg cgccacaaaa
      601 tgtttaagcg tacatataat attgttttca ctaaaaccct ttacataagc aacccccatc
      661 cggccgacga gataagggtc ttccccatat gtctcttcga cccttcccca tcttagttcc
      721 cgagcaagat cgagatcggg cgagaggacc tggtcgattc cctgggcctt caattccccg
      781 gtgatcgttc tggtcatttc attgatgagc gaaggattga acgtactgcc aagagcaatg
      841 gcctgcgggt agatggtcgc gccgccatgt acacatccat gcaacgcctc ggatgtcgcc
      901 agcatcggga tgccaagacg cgtcttttct acgagatatt tctgcacccg gttgacatcc
      961 ccggcatatt tttcggccgt cagccccatg ccttccatac aacccctgct cattccccca
     1021 aggaattttt ccaatttttc ttcatcgagt gaatccccgg tataaatatc gctaaaggag
     1081 agatcctgca tttgcgcgat cttttcgtgc agcgtcattc gctgcaaaag atcctttacc
     1141 ctggactcca ccggcgccga tgggttttta tacaccggca gtggtttgct ttgggcaacg
     1201 gcaaggaccg gaagaagaag caatacaaat gctacattaa ttttcatcgc aagcaatttg
     1261 actatcagat gcctttaact tttcgaaatt aactcaactt gcttcatgag gataatacta
     1321 ttcgttgcaa ttttgatatt atccttcatt ccacgctctt cgcagaaagc tgatccagtt
     1381 tttataaaat atattttcga tatcggcctt tgcatatccc ctgcgcgcaa ggatgtcctg
     1441 gtatttttga agatcggcga tgctgttcag atcccatggc gcctgttctg ttccgaatat
     1501 gccatccaga tcactcccaa aagctatatg attgctgttt cccgccatct ggcagatatg
     1561 atcccagtgg tccacgagat tttccagccg gatatccagt tgccagggat cggagaccgt
     1621 atcgataaat cgctgatcca tcgcccagca atcaagcata ccgccgatga ccgcgccacg
     1681 ttggatgagc agccttattt gttcgtccgc gagctgccgt tggttgggga cgatcttcct
     1741 tatattatga tggcttgccc agatgggtcc ctggtataga tcgattgcct gtaaaaaacc
     1801 ttcgtctgtg agatgcgtca cgtccaggat caggttcagc ggctccattt ccttcagcaa
     1861 ttggataccc gcggcggtca aaggtccttg caactttgta ccgggagcat accggcccgg
     1921 accgaaatgc gacaagccca tggcccgcaa gccatacgcg tgtgcggcgt ggaggtaact
     1981 gatatcgacg agggaatcgg cgccctccag gctaaggata taaccaatgg gtttttgctc
     2041 gtcttctaca gcgccgtcgt cccatagtgc caaatggtga tccagctgtt cgatattggt
     2101 gataggaacc atttccccca acttttccat ttcacggtac cagcacaatt gtgcctgcgt
     2161 catagcccat gcctgctgcg gagaattcca gccggcgaag ccgctgcccg gaggagtgta
     2221 acgcgccagt tgcgtggcga cgaccagccc caccctgccc ttacggagct cgggaaaaca
     2281 caccgtccct ttaccccggt ccggtttatc cttcatgttc atctctcttt gccggatctc
     2341 ggccaaaggg cgggtaagat ccctgttcca ttcgatggcg ttcatcgcaa gatcgagatg
     2401 ggcatcgacg atcaatggtc ggttcataca ctatattatc agacctggta attgccagtt
     2461 ggtttttgta tcccgttgaa tgggttcttc tctatgattt tctggatctg gtaactaaat
     2521 aggctgaata gctcattcca tcgccgggcg tcgcccggtt cataattata gaagccttta
     2581 ttgttctgca ttcccagtcc gccttgatcc gttatctctt cgaaaaaccg ggggatatgg
     2641 gcttcttttg ataattcccg gttcagatct ttcatcacca atccataggc ataggtcccc
     2701 atcagatcca tatacctgaa atttcctgca aacggaagat agtatcccgc atcgttccgg
     2761 caagctctgt cgatgtcttc gatcgtagca taaccgtttt ggaccagaaa aaaagcttcc
     2821 ctgatcattc cgctcagcat ttttgcgcgg atgccgacat ccttggaaac acaatagggg
     2881 tctttgttcc aatgctcttt tgccaggtca tacagatcat cgacgagctt ttgcctggta
     2941 gcagcgttgg taatgatctc gagaaaaaaa gtggtatgag cgggctccgc ccaattgagc
     3001 ccgataagcc gctcggggtg ttcgcagccg tcctgtaaca agctgagggg gatgctctcg
     3061 gtatttacag cgatgatcga tccaggaaaa agcagctttt ccagttgttg gatcctggcc
     3121 tttttttcat tcacattttc tcccgtgatc gctatcccga tcatgtggcc ggcggcttta
     3181 cgatcaaaaa atccttctat ctcgagcata tccggggtaa ggcttttccc attcagctcc
     3241 gatagacggg caaagtggct atttacgcca tcgaccgctt cctccttatc ctccgtcatt
     3301 aacgtaacgg catgccctgc ctgtaacagg caaacagtga cgctgcttcc cagtcttcca
     3361 ttggcgatca gcaatatggg tgatcccttg tatttagttt ccatacgaga ttgtgatgcc
     3421 caaagctaat cgttcccctt tccgatctat tccattatta tttgcatttc tgatactatt
     3481 tttcgcgggc gggcaaagtc ttggagagag gagcggaccc taccccggtt ggatgcatcc
     3541 ggagctagcg gcgttgcaaa aaaaagggga acggccgccg atcctgcggg tggtaaagaa
     3601 agaatgaacc aacgagccgg atacgaagaa ggaattcgtg atacaaaagt attcgacgcg
     3661 aagaaaacat tggtggttat gggaaacctt aaaggtaaaa acaaagcttt tccgtaagat
     3721 aatctatcat tttccctttt ttgtccattt gccacgaatg gaataaagct acatttgcga
     3781 ggaaaacggg aaaacattat cctgaaactc cgccagcctg gccgttcaca ggcaggtaaa
     3841 ataaccgctt cctcagttac cgaatcaatt ctattcttca gaatgggctt tggccttctg
     3901 actttcaatt tgggcgcttc gccaccttca tcctagtttt tcatggtaag gcagaaaggg
     3961 tctttgggat tcccgaagag atcgcgtgcc cgggaaacat gtgtttatat cagggttgcc
     4021 ggagtttgac cggatcaatc cgggccataa tgatttaaac tgcaaatgac attttatcaa
     4081 aacatacagg aaacggttac gttgcacccg tctgacaagc aactgcttca gcgaatggct
     4141 gatagtgagc gtcaggcatt taccttattg tatcgccgct attgggagga gctatttatt
     4201 accgcagcca gggcgttgcg caggaaagaa gaagccgccg atgtggtgca ggacgtcttt
     4261 ctttctttgt ggcatcggcg taacgacctg gatgttcaag gttcgctttc ggcttatctt
     4321 cacacgagtg ttcgctataa atgtatacac tatgtcgaga agaatatcac ccgccgcgac
     4381 tatcttattc aatttgcgga atcggtcaga agcagcgctt ccaaaagcgc cgaaatcaat
     4441 ttacaattaa aagaaataga gcaaacagtc aacaagacgg ttgcgaaaat gcctgctaaa
     4501 atgcaggaag tttacaaact aagccgccag cagcatttga gctataaaga aatttcagat
     4561 tccatgagca tttctgtaga gaccgttaaa aaacatatcc accacgccct gcatcttata
     4621 agaaaagacc tgcttcctta tctatttatc atcgcaagtc tggtgtttca ctttcaatgg
     4681 atctttttct ccctataatg tttcggagaa aggcccatta tcttcgtaaa cattcttgca
     4741 aaatagtagg cgtcttcaat acccagttcc agcgctattt cacgtatcct catatcggta
     4801 aaaagcaaat attggcaggc tttctgtact ttgaggtggt tgaaatattc cataggcggg
     4861 aagcccatcc tggccttgaa taagaatgaa aaatgtgaag gagataaatt aacggccgag
     4921 gccatcgctt caagcgtcag catttcgctt atgttcttgc ttaaaaaatc tatagccttt
     4981 tccatgacat cgccgttctt gatcttaatc ccgggttcat atctatcatt gtaaatcacc
     5041 gtggtaaaaa aatgacccag gcacatattg caatatatta aattatcgat gccatagcct
     5101 cgctggagct ggccgtatat ctcatcgaaa agagcgatac tttgccgatc atattgaata
     5161 aagcctttgt aaccgttaac ctgtttgtag aataaatcga taatggaatc ggagatgctt
     5221 cctttgaaat gtatccagta aattgtccag ggattatttt catttgcgtg gtaagtatgg
     5281 ggcgtatgca tcgggacaat gaaaaaatca ccggcttcaa tttcgtatgt ttcctttttt
     5341 attacagctg tcccgctgcc tgcctggcaa taaatcaaga tatgctgttc cgccccgcgc
     5401 ggcctttcgc ggtaatgaaa tcttgcctta ggataataac cgatatcggt tacatacagg
     5461 ttattggtta tcttattgcg ttcgcaatat tgggacaaaa tatttctggg aagtacaatc
     5521 gccttttggc cctgaaaacc ctcttttttt ctcacagtag cattttttat tcatctataa
     5581 taatcaaaaa gtccatcaaa ataaatgttt actcc
//
LOCUS       DASYSV010000493         2560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1127123, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000493 DASYSV010000000
VERSION     DASYSV010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127123"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VGQ51_15175"
     CDS             <1..754
                     /locus_tag="VGQ51_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEV9037976.1"
                     /translation="SLNRYPNGITGKSFYQKDVSATAPDWVIQMPYHTSTGEDKNFLV
                     PEDEASLLWMANLGAIEMNPWNSTIDKPDNPDWCIIDLDPTEDNTFGQVIRTAQVTRE
                     VLDELGVPGYPKTSGSTGIHIYIPLGARYSYAECQAFGKLIATRVHGRLPKITSIQRL
                     TQRRKGCIYVDYLQNRPKATLAAPYSVRPKPGATVSMPLHWEEVKKGLRMSHFTMKNA
                     MARIKTEGDLFAPVLGKGIDMRKILKTALAAG"
     gene            complement(757..1998)
                     /locus_tag="VGQ51_15180"
     CDS             complement(757..1998)
                     /locus_tag="VGQ51_15180"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEV9037977.1"
                     /translation="MNKKPDPAKAKRTRPKKYRVRKIVLIVIGSLILILIALRIALPS
                     ILLRYVNKELTTIPGYTGHVDDIDVHLIRGAYTIRVIRLDKTGGKIPVPFFSADAADL
                     SVEWGALFHRRLVGKIIVSHPILNFAKGPTKETSQTDIPTKPWTQVVSNLMPLKLNRF
                     EIFDGEVHYRDFYSTPKVNIYATDIHILAENLSNARHQKEQLPSTVEATCNGIYGGHA
                     WLKMKLDALNNAPTFEAKAELTNMDITRLNDYLSAYGKFTVKQGTIGIYTEAAAKDNK
                     IVGYTKPIIRDLRVVNWDKDKAHPLKLAWEAMIGAVNWIVKNKGKDQTATRVEFSGDL
                     KNPDVNVWYIIAQTLRNGFIQALYPALEESVNINSVDRIKDSPLKAEFEKGKKSKKDI
                     RREKRAEKRKKKEEKKKQEKN"
     gene            complement(2051..2482)
                     /locus_tag="VGQ51_15185"
     CDS             complement(2051..2482)
                     /locus_tag="VGQ51_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEV9037978.1"
                     /translation="MQSKLLNEGPEKTYAVILQYGEEAMEQLKKFATKEKLFACRFTA
                     IGAFSSADVGFFDFAIKDYQRIRIEEQTEVLSLVGDISLYQDKPKVHAHVVLGKRDGT
                     AHGGHLLTGIVNPTLEIILTESPVWMQRTVDEKSGIPLIRL"
BASE COUNT          515 a          713 c          695 g          637 t
ORIGIN      
        1 atccctcaac cgatacccca acggcatcac ggggaaaagc ttttatcaga aggatgtctc
       61 ggccacagcg cccgattggg tgatacagat gccttatcat accagcaccg gggaggacaa
      121 gaacttcctc gtgccggaag acgaagcgag tcttctctgg atggcgaatc tgggcgccat
      181 cgaaatgaat ccctggaaca gcacgatcga caagccggac aaccccgact ggtgtatcat
      241 cgatctcgat ccgacagagg acaacacttt cgggcaggtg atccggacag cgcaggtgac
      301 cagggaggtg ctcgatgagc tgggtgttcc cggttacccc aagacctccg gctcgacggg
      361 catccatatc tatatcccgc tgggtgccag atacagctat gcagagtgtc aggccttcgg
      421 caagctcatc gcgacgcggg tacacggacg gcttccgaag atcacgagca tccagcggct
      481 gacccagcgc cggaagggat gtatctatgt ggattacctg cagaaccggc ccaaggctac
      541 actcgccgca ccctattccg tacggcccaa accgggagcc accgtcagta tgcccttgca
      601 ttgggaggag gtaaaaaagg gattgcggat gagtcacttt accatgaaga atgcgatggc
      661 gcggatcaag acggaaggcg atctgttcgc gccggtgctc ggtaagggca tcgatatgcg
      721 gaagatactg aagacggcgt tggcagccgg ctgatgctaa ttcttttctt gcttcttttt
      781 ctcctccttc ttcttccgct tttctgcccg tttctcgcgg cggatatctt ttttgctttt
      841 cttccctttc tcgaactcgg cctttagcgg cgaatccttt atcctgtcga cggaatttat
      901 attgaccgac tcctcgagcg ccggatataa agcctggatg aatccattgc gcaaggtttg
      961 cgcgataatg taccaaacat tcacatccgg gtttttcagg tcgccggaaa attcaacccg
     1021 ggtggcggtc tggtccttcc ccttattctt cacgatccag ttcaccgctc cgatcatcgc
     1081 ttcccaggca agtttcaggg gatgcgcctt gtccttgtcc cagttgacca cccggagatc
     1141 cctgatgatc ggctttgtat agcccacgat cttgttgtcc ttcgcggctg cctcggtgta
     1201 gatcccgatc gttccctgtt tgaccgtaaa cttcccatag gcgctgagat aatcgttcag
     1261 cctggtgatg tccatattcg tcagctcggc tttcgcctcg aaagtggggg cgttattcag
     1321 cgcgtccagt ttcatcttca accaggcatg accgccatag atcccgttgc aggtggcctc
     1381 caccgttgac ggcagttgtt ctttctgatg ccgggcgttc gagaggttct ctgccaggat
     1441 gtggatgtcg gtggcataga tgttgacctt gggggtgctg taaaaatcgc ggtaatggac
     1501 ctcgccgtcg aagatctcga accggttcaa tttcagcggc atcagattgc tgaccacctg
     1561 tgtccatggt ttcgtcggga tatccgtctg ggaagtttct ttggtaggtc cttttgcaaa
     1621 gttcaggatc ggatggctga cgatgatctt tcctaccagc cgtcggtgaa aaagcgcgcc
     1681 ccattccacc gaaagatcgg ccgcatcggc cgaaaagaag gggacgggta tcttgccgcc
     1741 cgtcttgtcc agccggatga cccggatagt ataggcgccg cggatcaggt ggacgtcgat
     1801 gtcgtcgaca tggccagtat atcccgggat cgtggtgagt tctttgttca catatctcaa
     1861 caggatagac gggagtgcga tccgcaacgc gatcaggatc aggatcaatg acccgatgac
     1921 gatcaggacg atcttacgca cccggtactt tttcgggcgg gtgcgttttg ccttggcggg
     1981 gtcaggtttt ttattcatgg tgcttcaatt ctgagaaatc gtcattggcc ggtgcttgcc
     2041 gccccaccgt tcacaatctt atcagcggga taccgctctt ctcatccacg gtccgctgca
     2101 tccacaccgg cgattcggtc agtatgatct ccagggtcgg attgacaatg ccggtaagca
     2161 gatggccgcc atgagccgtc ccgtcgcgtt tgcccaatac gacatgggca tgaaccttgg
     2221 gtttgtcctg gtacaggctg atatccccca ccaggctcag cacctcggtc tgctcctcga
     2281 tcctgatccg ctgatagtcc ttaattgcaa aatcgaaaaa acccacatcc gcgctgctga
     2341 aggctccgat ggcggtaaac cggcaggcga acagcttttc tttggtggcg aatttcttca
     2401 gctgctccat ggcctcctcc ccatactgga ggatcaccgc gtaggtcttt tccggtcctt
     2461 cgttcaatag tttgctttgc atgacgttga gctccgtttt ccggtaagca cgtaattatt
     2521 gtgccacgcg ggtatggcaa ttgcgggcca tctgtgtatg
//
LOCUS       DASYSV010000494         8496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2023552, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000494 DASYSV010000000
VERSION     DASYSV010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023552"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VGQ51_15190"
     CDS             complement(<1..233)
                     /locus_tag="VGQ51_15190"
                     /inference="COORDINATES: protein motif:HMM:NF037701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037979.1"
                     /translation="MLKIFLVLWLCLTYREGFTQSLFVGAGDKHISYEGRIRMTKDVV
                     ELIWPGTSVRIDFEGTGLSGELKDQDTSNYYNI"
     gene            complement(335..886)
                     /locus_tag="VGQ51_15195"
     CDS             complement(335..886)
                     /locus_tag="VGQ51_15195"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV9037980.1"
                     /translation="MKTATHHLAAAVLVLLCSSFTVHETPPSAAEVVKQACTEASASH
                     KKVMILFHASWCTWCHKLDTMMASPECKSLFDDSYVIRHLTIAETPERKAEENPGAEA
                     LYEKYADQNSGIPFFLIMTADGTVIADSRIKPHGAKPGSSGDNIGYPASKEEVGYFIR
                     VLHETSGLTTSQLRTIREKLSKK"
     gene            complement(959..1960)
                     /locus_tag="VGQ51_15200"
     CDS             complement(959..1960)
                     /locus_tag="VGQ51_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037981.1"
                     /translation="MKLTFFLIALFLVLVRLCLPGLHLWHHGPNDGTIVVSGDDYREE
                     VKWSGKCRLSDDERSIAEMKPGGYLKFKENDTTFLAESNMQGEISYTLYNGREYLNAR
                     DTAFIAAQIRKMIGLGLFADGRAERILKKGGNKALLAEIARMRIEGVGSPYVNLLLKS
                     DSLTAEERIGLLRLVARDGDNGAAGRVLRAFNREQLRDSAVALAWLGDVEEMNDAGAR
                     REVLVNFLRSDTVNGSWLQAGVYDSILVSTGRWDAEFDQKAVYEMLTVLPGKTESQWI
                     ALIRTVSRLADDMQKSELLQKIWSAGPKSEALKAEFIQAALTIHDDWQYGKVMRVIE"
     gene            complement(1965..2399)
                     /locus_tag="VGQ51_15205"
     CDS             complement(1965..2399)
                     /locus_tag="VGQ51_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037982.1"
                     /translation="MKKAFLFATFLAGSVATYAQDKPEPLVNRELLFNILNTCSLVFV
                     VYLIANFIVRIVKYSFDYRLKSKIVDKQTQEGIVSQLVQPEKTNTRRAILQWFCTLLG
                     VGIGFTIMTVTQPLGLHSLAIMAFSVAAGLGSYYYFGKQVND"
     gene            complement(2406..2957)
                     /locus_tag="VGQ51_15210"
     CDS             complement(2406..2957)
                     /locus_tag="VGQ51_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037983.1"
                     /translation="MSEKHLNEDMLQQFALEPASCPPNVIAHLMECPECFLEATGYHQ
                     LGQALKEQPAPEFGFDLAAAVMGQLETGQGAAAPMEEVAELRPEVGGMGAPTGAAVLE
                     TPKGVVVTVREPRRISRVIVPVVIAIVAALPAWLFRRTAYFVFADVSEAIAWIILAAA
                     GIAVVFTVYKYYRRYQQVLHLIN"
     gene            complement(2954..3562)
                     /locus_tag="VGQ51_15215"
     CDS             complement(2954..3562)
                     /locus_tag="VGQ51_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9037984.1"
                     /translation="MTTRSNLPVNHQPGQQSTDRQLVELVLGGDRQAFSRIILATEGL
                     VAQIVFRLIPNEEDRKDVAQDIYLKVFHYLKGFKFQSKLSTWVGRIAYNTCLNRLDKK
                     QPDLCIAPAHDDEGKEVFRAAEETDRLVLQKELRGLIAREIARLPPLYQTLIGLYHQQ
                     ELSYQEIGAITGLPEGTVKSYLFRARRILKETTLSHYKKEKL"
     gene            complement(3559..3702)
                     /locus_tag="VGQ51_15220"
     CDS             complement(3559..3702)
                     /locus_tag="VGQ51_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037985.1"
                     /translation="MELAWVKAAEAMSAVKKMAVFRAAKKMVIFLGGEEIGVFPKFIA
                     TFE"
     gene            complement(3683..4453)
                     /locus_tag="VGQ51_15225"
     CDS             complement(3683..4453)
                     /locus_tag="VGQ51_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV9037986.1"
                     /translation="MGLSDDISLAQWSLVDEWRAGKWKTLDFPRLARNDFDLNGVEFV
                     NTLFEVPTEGYLKQLKQNAATHGVTMVLIMVDEEGDGCSPTREGRREFEVFHRKWVDI
                     AHYLGCHAIRTNCRGPRAANKDEALHWAAESYRPLLDYARPAGIDILIENHGGLSNDA
                     EWMVRLMKEIGDPLLGVYPDWREPGPEFDNFSFLEKTLSFARGMSYRNQPTEELTLKM
                     LGLCRDGGFHGWYGIESSGRGQIQQGIRILKENGIGLG"
     gene            complement(4500..6887)
                     /locus_tag="VGQ51_15230"
     CDS             complement(4500..6887)
                     /locus_tag="VGQ51_15230"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9037987.1"
                     /translation="MWKNYFTVAWRNLLKNKLYSLVNIAGLSTGMAVAMIIGLWIYDE
                     VSANRQFKNYNQLYEVMMHQTFDVVRGTQQALPYPLGEELKTKFPDFKGVAMCDWGSQ
                     RSLVYGEKKISKFGHFIGEDAVDMFSMKILEGDRHPLHDPYSIVLTDETAKILFGKED
                     PIGKAVKLDNQTMLKVTAVVEKQPKNSSLQYDYLIPFQLNELIYPWIKQFHKTNWGNN
                     SWEAYVQLNDNANPDAVQRKVKNVVISHFTDENTLKHIKPELILHPMSKWRLYSDFEN
                     GINTGGFIKYVRMFGILGFIVLFIACINFMNLSTARSEKRAKEVGVRKAVGSGRKQLI
                     SQFLGESLLISGIAFVLALAIVFISLPYFNKLTDKDMSLQITSPLFWIVMLAFTAVTG
                     LLAGSYPAFYLSSFNPVRVLKGHLQAGKSSSLPRKILVVVQFASSVVLMIGTIIVNQQ
                     IQFGKNQPIGFNNKGLISVYWSDDIGKNRDFIENELLRTGVVSSVTQSNSPPSEIYSN
                     NNGWEWKGSTPVEKAGLFSTITTTYHYTQTLGIKLVAGRDFNKDFASDSNGVILNEAA
                     VKRMRLKNPVGEVLKWNDKPMVVLGVIPDIQMESPYRPISPLTIVFSKGWVGCLDIRI
                     NPNVPVSKAIARIKPIFDKYNPAYPFEYHFADEEYAKKFNYEELVGNLAGIVAALAIF
                     ISCLGLFGLASFTAEQRVKEIGVRKVLGASVFNLWRLLSKDFVLLVLISCAIASPIAW
                     YYMREWLKGYKIATTIGLSTFVFVALIAMLITLITVSFQAVKAAMANPVKSLRTE"
     gene            complement(7251..8237)
                     /locus_tag="VGQ51_15235"
     CDS             complement(7251..8237)
                     /locus_tag="VGQ51_15235"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HEV9037988.1"
                     /translation="MKTKLYILILLTLISTGLFAGPRPRITAKTNGGNWSATGSWTPA
                     RIPQDGDSVVIPAGYTIILNNSYTLTNVYIAIEGTLNFNQNNTLALDIASIVNILSGG
                     TLTATHPTPNELLTINGVTKYDGKTDGTINGPLAATSTTGSSPSGFTMVILPVNFVSF
                     SAHRSNGSIQLVWNTANEMNNSHFEIERSANGSDWETIGNVTAGTSSSDKYTYTDETA
                     PAAETQYRIRQVDIDGNYGYSKVALVGGNATAAAQATIIASGKTVSIFSSNASGSRLI
                     VRVITIGGQVLQQQAFEATGRIDLSVPAAITGVYVVQVTDGMQWSVVKKVML"
BASE COUNT         1995 a         2408 c         2033 g         2060 t
ORIGIN      
        1 atgatattgt aataattgga cgtatcctga tccttcaatt caccggataa ccccgtaccc
       61 tcgaaatcga tcctgaccga agtgccgggc cagatcagct cgacaacatc cttcgtcatc
      121 cgtatcctcc cttcataaga aatatgtttg tcgccggccc ccacgaataa tgattgcgtg
      181 aagccttcac gatatgtcag gcaaagccag agaacaagaa atatcttaag catagcacac
      241 aagatacgac tattgacaac atcgccccac ctcccggcga cgaccccagc cgacccctcc
      301 tatcccctgc ggcgcccacc ccagcctcgt ccgcctactt cttacttaat ttttcccgta
      361 tagtcctcaa ctgagaggtc gtgagaccag acgtctcatg tagaacccgt ataaaatacc
      421 caacctcttc tttcgatgcc gggtacccga tattatcgcc ggatgacccg ggtttcgcgc
      481 catgtggctt gatacgcgaa tccgcgatga cagtcccatc ggctgtcatg atgagaaaaa
      541 aggggatgcc ggaattctga tcggcatact tttcatacag tgcctcggcg ccgggatttt
      601 cctcggcctt cctttccggt gtctctgcga tggtcagatg ccggatgaca taagaatcgt
      661 cgaacagcga tttgcattcg ggactcgcca tcatcgtatc gagcttgtga caccatgtac
      721 accaggaggc atgaaaaagg atcatgacct tcttatggct ggccgatgct tctgtacagg
      781 cctgcttcac gacctccgca gcagaaggcg gcgtctcgtg gacagtaaaa gaggaacaaa
      841 gcaagacaag tacagcagcg gcaaggtggt gagttgcggt tttcatgccc taaatttagc
      901 aagttttgta ccattataac gcccccgccg gcgacccttc atcacttcgc ccgacgcatc
      961 actcgatcac ccgcataacc ttgccatatt gccagtcatc gtgaatggtc agcgcggcct
     1021 gtataaattc ggcttttaaa gcttcgctct ttggcccggc cgaccatatc ttttgaagaa
     1081 gctcgctttt ttgcatgtcg tctgccaacc ggctcacggt cctgatcagc gcgatccact
     1141 ggctttccgt cttcccgggt agcaccgtca gcatttcata gacggccttt tgatcgaatt
     1201 cggcgtccca gcggccggtg ctcaccaata tcgagtcata cactccggcc tgcagccacg
     1261 agccgttcac ggtatccgat cttagaaaat taaccagcac ttctcttctt gcccctgcgt
     1321 cgttcatctc ttccacatcc cccagccagg ccagggcgac tgccgaatca cgtagttgtt
     1381 ccctgttgaa tgccctgagg actcttcctg ccgcaccgtt gtcgccgtcg cgtgccacca
     1441 gccgcaatag cccgatccgc tcctcagccg tcaggctgtc gcttttcagg agcagattga
     1501 cgtatggcga tccaacccct tcgatccgca tccgtgctat ctcagccaat aacgccttgt
     1561 ttccgccttt tttcagtatc cgctccgccc ggccatcggc aaagagtccc agcccgatca
     1621 tcttgcggat ctgcgcggcg atgaaggccg tatccctggc attcaggtat tcgcgaccgt
     1681 tatacagcgt atagcttatc tccccctgca tattgctttc cgccaaaaag gtggtatcgt
     1741 tctccttgaa cttcaggtat ccgcccggct tcatctcggc aatggaccgt tcatcgtcgc
     1801 tgagacgaca cttgcccgac cattttactt cttccctgta atcatctccg gatacaacga
     1861 tagtaccgtc attgggtcca tgatgccaca agtgcaatcc gggcagacag agacgcacca
     1921 ggaccaggaa aagggcgatc aggaaaaagg ttagtttcat acgatcagtc atttacctgt
     1981 ttgccaaaat aataatagct gccgaggcct gccgccacac tgaaggccat gatcgccagc
     2041 gaatgcagtc caagcggctg cgtgacggtc ataatggtga agccgatgcc gactcccagc
     2101 aaggtgcaaa accattgaag gatcgcgcgc ctggtattgg tcttctcagg ttggacgagc
     2161 tggctgacaa taccttcctg cgtttgcttg tcgacgatct tgctcttcag acggtagtcg
     2221 aaactgtact tgacgatcct gacgatgaaa ttggcgatca ggtagacgac aaagaccaag
     2281 gaacaggtat tcaggatgtt gaaaagtaat tccctgttga ctaaaggttc gggcttatcc
     2341 tgagcgtaag tcgccacgct tccggccaaa aaggtcgcga atagaaaggc ttttttcatg
     2401 ttttatcaat ttatgagatg aaggacttgt tgatatctgc gatagtattt gtacacggtg
     2461 aacaccaccg caattcccgc ggcagccagg attatccacg caatcgcctc cgacacatcc
     2521 gcaaaaacga aataagccgt cctccggaat aaccacgccg gtaaagcagc cacgatcgcg
     2581 atgaccacag ggacaatgac gcgtgagata cgtctcggtt ctctaacggt caccaccacc
     2641 cccttcggcg tttccaaaac cgccgccccc gtcggagctc ccatcccccc cacctccggc
     2701 cgcaattccg ccacttcctc cattggagct gccgcccctt gtcccgtttc caattgcccc
     2761 attaccgctg ccgcgagatc gaaaccaaac tccggcgccg gctgttcctt tagcgcttgc
     2821 cccagctggt gataccccgt cgcttcaaga aagcattccg ggcattccat gagatgcgca
     2881 ataacgttcg gcggacagga agccggctcc agcgcgaatt gttgcaacat gtcttcattc
     2941 aaatgctttt cactcatagc ttctcttttt tatagtgcga taaggtggtt tcttttaata
     3001 tcctgcgggc gcgaaaaaga tagctcttga ccgtgccctc cggcagaccg gtgatcgcgc
     3061 caatctcctg gtagctcagc tcctgttgat gatatagacc gatcagcgtc tgatacagcg
     3121 gcggcaacct ggcgatctct cgcgcgataa gtccccttag ctctttttgc aggaccagcc
     3181 ggtcggtttc ctcagccgcc cgaaaaacct cttttccctc gtcatcgtgc gccggtgcga
     3241 tacagagatc gggttgcttt ttatccagcc ggttcagaca ggtattatag gcgatccgcc
     3301 cgacccaggt cgatagcttc gactgaaatt tgaacccttt caggtagtga aaaactttca
     3361 gatagatgtc ctgggcgaca tccttccggt cttcttcatt ggggatcagc ctgaacacga
     3421 tctgcgccac caacccctcc gtcgccagga tgatgcggct gaaggcctgc ctgtcgccgc
     3481 ctagaaccag ctcgactagc tgcctgtctg tggattgctg ccctggttga tggttcacgg
     3541 gaagattaga ccgggtggtc attcaaatgt tgcaataaat ttcggaaaaa cgccaatttc
     3601 ttcgccgccc agaaaaatca ccattttctt cgccgcccgg aaaaccgcca ttttcttcac
     3661 ggccgacatc gcttcagccg ccttaaccca ggccaattcc attttccttc aggatgcgaa
     3721 taccctgttg gatctgcccg cggccactgg attcgatgcc ataccagcca tggaaaccac
     3781 cgtcccggca aagcccaagc atcttcaggg tcagctcctc ggtcggctgg tttcggtagg
     3841 acatgcctct ggcaaaggag agcgtctttt ctaagaagga aaaattgtca aactccggcc
     3901 ctggttcgcg ccaatccgga taaacgccta ataagggatc gccgatctcc ttcataagcc
     3961 gtaccatcca ctcggcatca ttggaaagac cgccgtgatt ctcgatcagt atgtcgatac
     4021 cggcgggtcg ggcatagtct aacaggggcc gataggactc ggccgcccag tgtaaggcct
     4081 catctttatt agcggcccgg ggaccgcggc agttggttcg gatggcatgg cagccgagat
     4141 agtgcgcaat gtcgacccat ttgcgatgga agacttcgaa ctccctccgt ccttcgcggg
     4201 ttggggaaca gccgtcgccc tcctcatcaa ccattatcag caccatggtc acgccatgtg
     4261 ttgccgcatt ctgtttcagc tgtttaagat aaccctcggt aggaacttcg aagagggtat
     4321 ttacgaattc cactccattc agatcgaagt catttctcgc cagacgggga aagtcgagtg
     4381 tcttccattt ccccgcgcgc cactcgtcca cgagcgacca ttgtgccagc gagatatcgt
     4441 cggataggcc catggccgac aaaatacaaa gagcttgctc ttctgcaaaa aaaattccat
     4501 tattcagtcc ttagactctt aaccgggttt gccatggctg ccttcaccgc ctgaaaactg
     4561 acggttatca gcgtgatcag catcgcgatc agggccacaa agacaaaggt actcagcccg
     4621 atcgtcgttg cgatcttata cccttttaac cattccctca tataatacca ggcgatgggg
     4681 ctggcgatgg cgcaggagat cagcaccaat agaacaaaat cctttgacag caaacgccat
     4741 aaattgaata cgctggcacc cagtaccttt ctgaccccga tctctttcac ccgctgttcc
     4801 gctgtgaagg aggcaagacc gaaaaggccg agacaggata taaagatggc aagcgcggca
     4861 acgatccccg caagattgcc caccaattct tcgtaattga atttcttcgc atattcctca
     4921 tctgcaaaat ggtattcgaa gggataggcc ggattgtatt tatcgaatat gggtttaata
     4981 cgagcgatgg ccttcgatac cggcacgtta ggattgatcc ggatatccag gcatccaacc
     5041 cagcctttgc tgaaaacaat ggtcagggga gagatcggcc ggtaaggtga ttccatctgg
     5101 atgtccggga tcacacccag cacgaccatc ggtttatcgt tccatttcaa aacttctcca
     5161 accgggttct ttagccgcat ccgtttgacc gcggcctcat tcagaatgac cccgttcgaa
     5221 tcggaggcga aatccttgtt aaagtctcgt ccggcgacga gtttgatacc gagcgtttgg
     5281 gtataatgat aagtggtagt gatcgtcgag aacaacccgg ccttttccac aggcgtactg
     5341 cctttccact cccagccatt attgttgctg tatatctcgg aaggtggcga attgctttgc
     5401 gtgaccgagc tgacgacccc cgtccggagt aactcatttt caataaagtc ccggttcttt
     5461 ccgatatcat ccgaccagta caccgagatc aggcccttat tgttgaagcc gatgggctga
     5521 ttttttccga actgtatctg ctgattgact ataatggtgc cgatcatcag cacgaccgag
     5581 ctggcaaatt gaacgacaac aaggatcttt cggggcaacg agctgctttt ccccgcttgc
     5641 agatgcccct tcagcaccct gacgggatta aaagatgaaa gataaaaggc gggataactt
     5701 ccggccagca gacccgtcac ggcggtgaat gccagcatca cgatccaaaa caacggactc
     5761 gtgatctgga ggctcatgtc cttatccgtg agtttgttga aatagggcag cgagatgaat
     5821 acgatggcca gcgcgagaac gaaagcaatt ccggagatca ataacgactc gccgaggaac
     5881 tggctgatca gttgtttccg gcccgacccg acggctttcc tcacccccac ttccttggca
     5941 cgtttttctg aacgcgcggt gcttaaattc atgaaattga tacaggcgat aaacaggacg
     6001 ataaaaccca ggatcccgaa catccggaca tacttgatga agccgccggt attgatccca
     6061 ttctcaaaat cgctgtacaa tctccatttc gacataggat gcaggattag ctccggtttg
     6121 atatgcttca gggtattctc atcggtgaaa tgggagatga ccacgttctt cacctttcgc
     6181 tgcaccgcat ccgggtttgc attatcattc agctgcacat acgcctccca ggagttattt
     6241 ccccaattcg tcttatgaaa ttgtttgatc caggggtaga taagctcgtt cagctgaaaa
     6301 ggaataaggt agtcgtattg gagactgctg ttcttaggct gcttctccac gaccgccgtc
     6361 actttgagca tcgtctggtt gtccagtttg accgccttgc cgatcggatc ttccttcccg
     6421 aataggatct ttgccgtctc gtcggtcaat acgatggaat aggggtcatg taacgggtgc
     6481 ctgtcgcctt cgagtatctt catcgaaaac atgtccaccg catcctcgcc gataaaatgc
     6541 ccgaacttgc ttattttctt ttctccatac accagcgatc gctgtgaacc ccagtcgcac
     6601 atcgccacgc ccttgaaatc gggaaattta gtcttcaact cttcgccgag aggatacggt
     6661 aacgcctgct gggtgccgcg taccacatca aaagtctgat gcatcattac ctcgtacagc
     6721 tggttataat tcttaaattg ccggttcgca ctgacttcat cgtatatcca cagcccgatg
     6781 atcatggcta cagccatccc cgtagacaat ccggctatgt tcaccaggga atagagctta
     6841 ttcttcagga gattcctcca ggcgactgtg aagtagtttt tccacataac cctaaggtcg
     6901 tagccgaacg cccggatgtt gcatccgctc ccccgaattt ccgatattgc actggaaatc
     6961 ttatgacaac tcacgaaaag atcgccttca agatgaagct aaaacatatc gggccatggg
     7021 gcagctcgaa aaggagatgt atggaaggca actggcggca tatgcgggtg agcttaaagc
     7081 gctccaatcg atcccggccg acccgatctg ccatcaggca ggtcccgggg cttttatccg
     7141 gggcgcaggt ttcgcttttt ttgtgagtac agggctggga aaattgcagg tggaaggact
     7201 taccgttctt tttgtctcgc ccctggcccc gcttgcccga tcacgacgaa ttacaacatc
     7261 accttcttca cgaccgacca ttgcatacca tcggtcacct gtacaacata tacaccggta
     7321 atcgcagcag gaacactaag atctattcgg ccagtggctt caaaagcctg ttgttgcaaa
     7381 acctgtccgc cgatcgtgat cacccgtacg ataagccggc tgccggatgc attcgaagag
     7441 aagatcgaca ccgtcttgcc gcttgcgatg atcgtggctt gagcagctgc agttgcgtta
     7501 ccgccgacca gcgctacctt gctatacccg tagttgccat cgatatccac ctgacggatg
     7561 cgatactggg tctcagcggc gggcgcggtt tcatccgtat aagtatattt atcggaagaa
     7621 gaagttcccg cagtgacatt accgatcgtt tcccaatccg aaccattggc actacgttct
     7681 atttcaaaat gactgttgtt catttcattt gcggtattcc agaccagctg tatcgatcca
     7741 ttgctgcggt gtgcagaaaa ggaaacaaaa ttaacgggca ggatcaccat cgtaaagccg
     7801 gaaggcgaag agccggtagt agacgttgct gcaagcggac cgttaatggt accatcggtt
     7861 ttgccgtcat atttggtgac gccattgatg gtaagcaatt cgttcggggt agggtgcgtt
     7921 gcagtcagag tgccaccgct caggatattg actatcgaag cgatatccag cgccagcgta
     7981 ttattttgat tgaaattaag cgtcccttct atggcgatat atacattggt cagggtatac
     8041 gagttgttca atataatagt ataaccggcc gggatgacga cagagtcgcc gtcctgaggg
     8101 atccgtgcgg gcgtccagct gccggttgca gaccagttgc cgccattggt tttagccgta
     8161 atacggggcc gggggccggc aaaaaggccg gtagagatca gggtcagaag gatcagaatg
     8221 tagagtttcg ttttcatggg ttccagggat tgttggatac accataaaag acaatcggca
     8281 taccaaaggt ctctttcgtg ccaaataccg gccgccaccc tcataaatag ctatatatca
     8341 accacttaca aaacaaattc aaaacccttc caaatgccgg aagaccccgg ggtctatagg
     8401 aaattttcca gttattggaa gatcgccccc gtttgggagc ttaaacggcc ttacgatgcg
     8461 tcggcctgcc tctcccccgc caccctcgcc agcctg
//
LOCUS       DASYSV010000495         7274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_484576, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000495 DASYSV010000000
VERSION     DASYSV010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484576"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..279
                     /locus_tag="VGQ51_15240"
     tRNA            193..279
                     /locus_tag="VGQ51_15240"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:227..229,aa:Ser,seq:cga)
     gene            546..1847
                     /locus_tag="VGQ51_15245"
     CDS             546..1847
                     /locus_tag="VGQ51_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV9037989.1"
                     /translation="MISKSFGLLFYLKKQHGDTKVHRPIYLRITVDGVMKEMSIHRSW
                     DPTKWNAKAGWAIVPKTPPRRSAAPPECEAQSLNAYLDTLQAKVHEARHQLLMSGKPV
                     TAEALRNFVQGREAGSERPHLLLEIFQYHNDQVKALIGKEFSKGTLTKFNTVLKHTRS
                     FLQWKYKLDDIDIRKLDYEFITELEFWYKSVQRVEHNTTMKYIACVKKMAIRALRNGW
                     LQRDPFMGFNMALREIEREALTVEELERMTTKSFITERLSQVRDIFLFSCFTGLAYAD
                     LQKLKRSEISTGIDGGKWIFTRRQKTDTASRIPLLPMAQQILDKYFDHPLCLKKDKVL
                     PILSNQKMNQYLKEIADVCQIDKYLTFHIARHTFATTITLSNGVPIETVSKMLGHRNL
                     KTTQHYAKIVDRKVSNDMQALKERLSLKPILKGQQEEKKTV"
     gene            1913..2341
                     /locus_tag="VGQ51_15250"
     CDS             1913..2341
                     /locus_tag="VGQ51_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037990.1"
                     /translation="MYKRGTFGVKNLKPPKSSSQAIIQQNKENAMTTSENQKEPQLNG
                     NSPTDRKNKGGRPKGGPGNTHHRLNIYVSDDEYKRFMEFVSNGWYDQNASSAGRYLPA
                     AALLNWQRGGRKPEWCPIKRILDLIFIAIIPVLKFRYKSV"
     gene            2510..2797
                     /locus_tag="VGQ51_15255"
     CDS             2510..2797
                     /locus_tag="VGQ51_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEV9037991.1"
                     /translation="MPKTYIYKFHVSGIYNSADVTAFRTRPETMPGVSDVNVDLRKMQ
                     AQITASMVIECPVLRKALDNTNFEMSGLTASVITPPCGVRDDEVEDPATPI"
     gene            3233..3511
                     /locus_tag="VGQ51_15260"
     CDS             3233..3511
                     /locus_tag="VGQ51_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037992.1"
                     /translation="MSTSASTTKDSGLSRIFRVDSSFHENQKAVANHKKAADHYHLAA
                     RSHLAAAHHHEAGAFQIAAEDTVAAYGHSLLAREYQKELAKNYALKNV"
     gene            complement(3538..4608)
                     /locus_tag="VGQ51_15265"
     CDS             complement(3538..4608)
                     /locus_tag="VGQ51_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV9037993.1"
                     /translation="MRTIITGTGSYIPGEVRSNQDFALVEFYNEHRQRIRVEGVELIG
                     KFELVTGIAQRRYAQEDMNASDMAALAAAAAIQDAGVDPESIDQLIVAHNFGDVLKHT
                     CQSDAVPSLATRVKRALAIRNPGCVAYDLLFGCPGWLQGLIQADAYIKAGLSSKCLVV
                     GAETLSRVIDPCDRDSMIYSDGAGAVVLEARCDAPADSGILGSISQTHAFVEGEYITL
                     GHSYSPAADSQRRYLKMQGRKVYEFALKTVPAAMKACLETCKIDIDQIKKIFLHQANE
                     KMDAAIVKAFYRLYGYKEAPSPIMPMNIRELGNSSVATIPTLFDMVRKAALPEHRLDR
                     GDTLLFASVGAGMNINAICYRM"
     gene            complement(4840..5379)
                     /locus_tag="VGQ51_15270"
     CDS             complement(4840..5379)
                     /locus_tag="VGQ51_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV9037994.1"
                     /translation="MVSLSSREIVKSELIRLGLHPISVDRCVADIWENPFQVQQHELR
                     TALAEAGFELLEDKKDILIQQIKNMIIEVVYDIEEPLVYNLSGYLSERLDHDYTYMSN
                     LFSERLDTTIEKFYIRHKIERVKDMLLYERLTLTEIAHKMHYSSLAHLSSQFKKITGL
                     TTSQFKQQNSSDRPMRADY"
     gene            5518..>7274
                     /locus_tag="VGQ51_15275"
     CDS             5518..>7274
                     /locus_tag="VGQ51_15275"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEV9037995.1"
                     /translation="MEENEDMTPSRPAPDLFPVVGVGASMGGLEAFKKLVRAIPQHPG
                     MAFILVQHMEPTHESLLPELLQKVTGIPIEEITDHVQVKPDRIYIVPANKVLIAVDGH
                     LQLSPRPPKNERNMPIDVFFTSLAVAHQSRAIGVVLSGNATDGTLGLKAIREQGGFTF
                     AQEPASAGARGMPESAIDAQVVDFILAPEDIPDQLAHLAEAFRSSPADDSPGIRPAWE
                     EAFRELLHSVFIRTGTDFTYYKQSTIRRRVVRRMGLNKIGNIAAYLAYFSENKAEQDL
                     LYQDLLIPVTGFFRDPEVYDLLKQSVFPGMLKTKDKGVPLRIWVAGCSTGEEAYSMGI
                     CLYECMGDRTMDSSFQIFASDLSERSIARARSGLYSRKDVSGLSDTLLQRYFVKMNGS
                     YQVNKQIRDVCVFACHNFLKDPPFAKMDLVSCRNVLIYMDPFLQKRALTTFHYALNEN
                     GYLLLGTSETVAPAADLYVPVGKHEKLYLRKPGRGRYVHQVTEGADKDYRKASYNTRK
                     DPPRDDFQKSADDTLLSRYTPPGVVVNEQLEIVQFRGSTGAWLEASPGKPSLNVLKMA
                     REGLAFELRNALHKARAGD"
BASE COUNT         1839 a         1862 c         1859 g         1714 t
ORIGIN      
        1 tcgatgatca atacggttcc agttgacatt ccttcttcga atttgccgcc aaaactatcc
       61 cttttccctg aatcgcctat aaatccccca tttttcgccc aaatccatta tttttgcctc
      121 ccgtttcgcc tcgcgccgcg cgcttcccgc gccgacgcct cgcaagtccc cgccgggcct
      181 gcgtcaaccc ccggagaggt ggcagagcgg tcgattgtgg cggtctcgaa aaccgttgtg
      241 cgggcaaccg taccgagggt tcgaatccct ccctctccgc agttggtttc tcaaactttt
      301 cgccgaaagc ctgttgaccg agtgttgacg ggctttttgc tttctagcag ccgccggact
      361 ttccgtcgtt ttgcctcact tttcttcgtt ttccagtgag caattcgtga gccgcttgaa
      421 attcgccgaa atcggctcac tggcgagcac caaaagccct gaacctcagc caagttgcgc
      481 atatttcgcc taagtcaaga acgtatgcaa ttgctaattt actcaccagt ttaaaagcac
      541 ttgttatgat ctcgaagagt tttggtttgc ttttctacct caaaaagcag cacggtgaca
      601 ccaaagttca ccggcctatt tatctccgta tcactgttga cggagtaatg aaagaaatgt
      661 cgattcaccg ttcctgggac ccgacaaaat ggaatgccaa ggctgggtgg gccattgttc
      721 ccaagactcc gccccgtcgc tcggctgcgc cgcccgaatg cgaagctcag tcgctaaatg
      781 cctacctcga caccctacaa gcgaaggttc atgaggcccg acatcagctg ctcatgagcg
      841 gtaagccggt caccgccgaa gccctccgca actttgtcca aggccgtgag gcaggcagtg
      901 agcgcccgca tttgttgctc gaaatcttcc aatatcacaa tgaccaggtg aaggccctga
      961 taggaaaaga gttctccaaa gggacgctga ccaagttcaa tacggtttta aaacataccc
     1021 ggtcattcct gcaatggaag tacaaattgg acgatatcga catccgaaaa ttggattatg
     1081 aattcatcac cgaactggaa ttctggtaca aaagcgtgca gcgtgtcgag cacaatacaa
     1141 ccatgaaata tatcgcctgt gtcaagaaaa tggcgatccg cgcgctgcgg aatggatggc
     1201 tccaacgcga tccgttcatg ggtttcaata tggcgttgag ggagatagaa agggaagcat
     1261 taacggtgga ggagttagag cgcatgacga cgaaaagctt catcaccgag cgcctaagtc
     1321 aggtccggga tatcttttta ttcagttgtt ttaccggtct ggcatatgcc gacttgcaga
     1381 agttgaagcg gtcggaaata agcaccggca tcgatggcgg caagtggatc ttcacccgcc
     1441 ggcagaagac cgacacagcc tcccgaatcc ctcttttacc aatggcgcag cagattttag
     1501 ataagtattt cgaccatcca ttatgcctga aaaaagataa ggtcctaccg atcctaagca
     1561 accaaaagat gaatcaatat ctcaaagaaa tcgctgacgt ctgccagatt gacaagtacc
     1621 taaccttcca tattgcccgc cacactttcg cgacaacaat cacgctgagt aatggcgttc
     1681 ctatcgagac agtttcaaag atgctcgggc atcgtaatct gaagacgaca cagcactatg
     1741 cgaaaatagt cgataggaag gtcagcaacg acatgcaagc gctgaaggaa aggttatcac
     1801 tcaaaccaat cttgaaaggc cagcaagagg aaaaaaagac ggtctaaaac catttttgcg
     1861 gcgtagaaga tcgcatctcg gtggaacttc agactctctg ccccctgccc agatgtacaa
     1921 acgtggcacg tttggggtaa aaaacctcaa gcctccaaag tcgtcttctc aagccataat
     1981 ccaacaaaat aaagaaaatg ccatgacaac aagtgagaac cagaaggagc cgcagttgaa
     2041 tggaaacagt cctaccgatc ggaaaaataa gggcggcaga cccaaaggcg gcccgggtaa
     2101 tacacatcat cggctgaaca tttacgtttc agacgacgaa tacaaacgct ttatggaatt
     2161 cgtttcaaat ggatggtacg atcaaaatgc gtcgagcgcc gggcgctatt tgccagccgc
     2221 agcactgcta aattggcagc gtggtgggcg aaagcctgaa tggtgcccta tcaagcgcat
     2281 cctggatttg atttttatcg caataatacc cgtcctgaaa tttcgctaca aaagcgtcta
     2341 aatcgatctt gcccatacta tcttataggc gggaaaccta tcatgaggtg agaattatgt
     2401 aacgggatac cttaactgta taatattttt ctcgaaggtc aaattatctt tggcagcacc
     2461 taccttcgcc cgcctactaa tccgaaaaaa taactaatga ggagagataa tgccaaaaac
     2521 atacatttat aaatttcacg tcagcggaat atacaacagc gcggatgtca ccgccttcag
     2581 gaccaggccg gaaacgatgc caggtgtcag cgacgtcaat gtcgacctgc gaaaaatgca
     2641 agcgcagata accgcctcaa tggtcatcga gtgccccgta cttagaaagg cgcttgataa
     2701 tacgaatttc gagatgtcgg ggctgacagc aagcgtaata actcctcctt gcggcgtaag
     2761 ggatgatgag gtcgaagacc cggccacacc tatttgaccc agaagggtcg aaagttttgg
     2821 aagaaataat attttatcct ttcctgaagc cccatccccg cgattgggag tccgcaattc
     2881 cttagatcca gtgcccgctc taactggcta ttagcaaacg cgtcctggtc ataaaaacta
     2941 gccttctcat ccatttcccg ttttttgctg tcagataaga aaagagcgta ttcgcctgga
     3001 gtatttttgt ccttgtgcat gcgattattt gaatgttgtc tgcaaaattg ccgcttattt
     3061 ccgtctatat cgttatacta ttgctggaat gagttacaac attcacacat ctttcggcag
     3121 gataggtgtg aatgatgcaa tgaaggatag gttttgtata gcaaagccat ggcatatatt
     3181 ccccaccttt gctatatcgg gaaaatgcac tcacgatata aatcgaaaat atatgtcaac
     3241 ttcagcatct acgaccaagg acagtggtct ctcaagaata ttcagggtcg atagctcttt
     3301 ccatgaaaat caaaaggccg ttgccaacca caaaaaggcg gccgaccact atcacctggc
     3361 cgcccggagc catctcgcgg ctgcgcatca tcatgaggcc ggcgcctttc aaatagccgc
     3421 tgaagatacc gttgccgcct atggtcactc cttactggcc cgcgaatacc aaaaggaact
     3481 ggcgaagaac tatgcattaa agaacgtata ggcgtcaaag gacgtgctga tcccctttca
     3541 cattctgtag catattgcat tgatgttcat gcctgcgccc acggacgcaa agagtagagt
     3601 gtctccccgg tccagacgat gctccggcag ggcagccttc cttaccatgt cgaacagggt
     3661 tgggatggtg gcgaccgagc tattgccgag ctcgcggatg ttcatcggca tgataggtga
     3721 aggggcttcc ttgtagccat agagccggta gaacgccttt acgatggccg catccatctt
     3781 ctcattagcc tggtggagaa agatcttttt tatctggtcg atatcgattt tacaggtctc
     3841 gagacatgct ttcatggcag caggcacggt tttgagcgca aattcgtaca ccttgcggcc
     3901 ttgcatcttg aggtaacgcc gttgggaatc ggcagccgga gagtaagaat ggcccaaagt
     3961 gatgtattcg ccttcaacaa aggcatgcgt ctggctgatg gagccgagga tgcccgaatc
     4021 ggccggggcg tcgcatcgag cctcgaggac aaccgcgccg gcgccgtcgc tatagatcat
     4081 actgtcccgg tcacagggat cgatcaccct gctgagggtt tcggcgccaa ctaccaggca
     4141 tttactgctg agaccggcct ttatgtaggc gtctgcctgg ataagtccct ggagccagcc
     4201 aggacaaccg aatagcaggt cataggctac gcaacctggg ttccggatcg caagagcacg
     4261 ctttacacgg gtggccaggc tggggactgc atcggattga caggtgtgct ttagaacgtc
     4321 gccgaaatta tgtgctacga tgagctggtc aatggattcc ggatccacac cggcgtcctg
     4381 gatggccgct gcggcggcca gggctgccat atcggaggcg ttcatgtcct cttgggcata
     4441 tcgtctttgt gcgatgccgg tgaccagctc aaattttccg atgagctcga cgccttcgac
     4501 gcggatacgc tgccggtgtt cattgtaaaa ttccacaagg gcgaagtctt gattagaacg
     4561 gacttcgcct ggtatatagc tgcctgtgcc tgttatgatt gttcgcataa ggtctcactt
     4621 atgggtgatc aaaaaaaagc gggcgaccgg gtaccgaccg cccgcggata ggatttagca
     4681 agtatacttc aacttctata aaaacaaatc ctgatcaaag ccctaaccta taaagggcaa
     4741 gggtcaaaga taacaacgag gaccggcttg ccacttacag gatttgcaat aaatgttaca
     4801 aaatttacag ttcatgaccc tcccatgcgt gggtccggat caataatcgg ccctcatggg
     4861 gcggtcgctg ctattttgct gtttgaattg ggaggtggtt agaccggtaa tctttttaaa
     4921 ctggctggag aggtgagcca ggctgctgta gtgcatttta tgagctattt cagtaagggt
     4981 cagcctctca taaagaagca tgtcttttac ccgttcgatc ttgtgccgga tgtaaaattt
     5041 ttcaatggtc gtatccagcc gttccgagaa caggttggac atgtaggtat agtcatgatc
     5101 caggcgttcg ctcaggtatc cggataaatt gtaaaccagc ggctcctcga tatcatagac
     5161 aacttcgatg atcatgttct tgatctgctg aataaggata tcttttttat cctccagcag
     5221 ctcgaagccc gcctcggcca atgccgttcg cagttcgtgc tgctgcacct gaaaagggtt
     5281 ctcccagata tccgccacgc atctatcgac gcttatcggg tgaaggccga gccttatcag
     5341 ttcgctcttc acgatctccc ggctgcttag gctcaccata tcttttatat agagtttcat
     5401 agcgattcaa acaaggacga ctgttcttgt aatttaccga aattttctta gattaggaat
     5461 aaccgtacct tacggcctga tgccttgtca caaaaagcac ctttacaatt acgtgtcatg
     5521 gaagagaatg aagatatgac ccccagccgg cccgcgccgg atttgttccc ggtggtgggc
     5581 gtgggtgctt ccatgggggg attggaggcg tttaaaaagc tggtcagagc catcccgcaa
     5641 catccgggca tggcctttat cctggtgcag cacatggagc cgacccatga aagcctgtta
     5701 ccggaactgc tccaaaaagt gaccggcata cccatcgaag agatcacgga tcatgtgcag
     5761 gtcaaaccgg accgcattta catcgtcccg gcgaataaag tactgattgc cgtagacggc
     5821 cacttgcagc ttagcccccg gccgccgaag aacgaacgga atatgccgat cgacgtgttt
     5881 ttcacctccc tggctgtggc ccatcaaagc cgggctatcg gtgtggtcct gtccggcaat
     5941 gccaccgacg gcacgctggg attaaaagcc atcagggaac agggaggatt tacctttgcg
     6001 caggagccgg cgtcggccgg cgcccgtggc atgccggaga gcgcaatcga tgcgcaagtg
     6061 gtggacttta tcctcgcgcc cgaagatatt cccgaccaac tggcccatct ggcggaggcc
     6121 tttaggagca gcccggcaga cgacagcccc ggcatcaggc cggcctggga ggaagctttc
     6181 cgcgaattgc tgcatagcgt cttcatccgc acaggaacgg attttaccta ttataaacaa
     6241 agcactatac gccggcgcgt cgtgcggcga atgggcttga acaaaatcgg gaatattgcc
     6301 gcctacctcg cttatttcag cgagaacaag gcggagcagg atctactata ccaggacctg
     6361 ttgatccccg tgacgggatt tttccgggac cccgaggttt acgatctttt aaagcagtcg
     6421 gtgttccctg gcatgttgaa gacaaaggat aaaggagttc ccctgcgcat ttgggtggct
     6481 ggatgctcca cgggtgagga ggcctattcc atgggcatct gtttgtatga atgcatgggc
     6541 gacaggacta tggatagcag tttccagatc ttcgcctccg acctttcgga acgatctatt
     6601 gccagggcaa gaagcgggct gtattcgcgc aaagatgtga gcggtctgag tgataccctt
     6661 ctccagcggt actttgtgaa gatgaacggg agctatcagg tcaataaaca aattcgggat
     6721 gtgtgcgtat tcgcctgtca caactttctt aaagatccgc cgtttgcaaa aatggacctg
     6781 gtcagttgcc ggaacgtgct gatctatatg gacccgtttc tgcagaagcg agccctgacc
     6841 acctttcatt atgccttgaa cgaaaatggg tatttgctgc tggggacctc cgaaacggtt
     6901 gcgcccgccg ccgatctcta tgtgccggtg gggaaacacg aaaaactgta tctccgcaaa
     6961 ccgggccggg ggaggtatgt gcaccaggtt acggaaggcg cggataagga ttatcggaaa
     7021 gcttcctaca acaccaggaa ggatcctccc cgggacgatt ttcaaaagag cgccgacgat
     7081 acgctgcttt ccaggtatac gccgccggga gtagtggtga atgaacagtt ggagatcgtg
     7141 cagttcaggg gatccacggg ggcctggctg gaggcttcgc ccggaaagcc gagcctcaat
     7201 gttttgaaga tggcgcgcga aggattggcc ttcgagctga gaaacgcctt gcataaggca
     7261 agggcgggtg acaa
//
LOCUS       DASYSV010000496         7993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2534646, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000496 DASYSV010000000
VERSION     DASYSV010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2534646"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..746)
                     /locus_tag="VGQ51_15280"
     CDS             complement(12..746)
                     /locus_tag="VGQ51_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037996.1"
                     /translation="MAITNAEAQQFNSDSWLSKPWGTVTLIPTIGQRNTMIMNTYSLF
                     PKWEFTMAAYLYNNDNNSTTNDGYSYSLYFKYMFCENKAQTGGIAVKAGTGMFPGTLN
                     AELRAEDAFKTYWMNVPSTIPFFNNTLSWDLMPGASYTNNYGEEGISGWAFTYSTRLA
                     WYPKGPAVSVVGEIFGAAGTTGTIPEYKIGLRWEPSPYSVFALTYGQEIGGSLGAGWE
                     LGVMLFTPPFACFGCKPAKSTAVFKQ"
     gene            complement(920..1153)
                     /locus_tag="VGQ51_15285"
     CDS             complement(920..1153)
                     /locus_tag="VGQ51_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037997.1"
                     /translation="MAKHTRDSAEAYFTDMEKMKFKYRVEKAVGDDTDVFLWYHIAMG
                     KKTIATCGWYHLINEKIAELRVLFDPRPLLPAA"
     gene            complement(1311..1592)
                     /locus_tag="VGQ51_15290"
     CDS             complement(1311..1592)
                     /locus_tag="VGQ51_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9037998.1"
                     /translation="MARKHSRIKLTDKSLQELKDKGFLYVLIKGYTPAQRNDRIELNQ
                     FILVPVRALPDAPGEKEIFAPIDSEVLTFWANPSEDGIEAYIELSLESA"
     gene            1714..2325
                     /locus_tag="VGQ51_15295"
     CDS             1714..2325
                     /locus_tag="VGQ51_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV9037999.1"
                     /translation="MSIKGLFPLHKWDFESGRVLADLPEQDLALLMSRKTESAYKKGQ
                     VIFREGGYPSGLYYVVNGRVKKYKSDHDGGEQIIYVAGTGELLGFHAILSGDNYPDSA
                     AVLSHEFVVLVNSLTMASRRPVRERLALQLIVVREKYKSETNNSPVEINLSRDDLASL
                     VGTARENVVRILSEFKEEGILSTRGRKIIVRDIKRLIEVANYK"
     gene            2339..2683
                     /locus_tag="VGQ51_15300"
     CDS             2339..2683
                     /locus_tag="VGQ51_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038000.1"
                     /translation="MGTPITNLLLSIMLSVVALSPADRLQAQTPARYKSYEGEYRSRD
                     DSDDMVRLIAADTGIVMKQLWDRKELRFYSVTDTYFYNAELRYALYILKDESGKAKSI
                     LLGRARFEKVGP"
     gene            complement(2708..3955)
                     /locus_tag="VGQ51_15305"
     CDS             complement(2708..3955)
                     /locus_tag="VGQ51_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV9038001.1"
                     /translation="MKTQPFILVTALVVLTTPIYAQGSKPVPEAALRARVDSIVQSAI
                     RADLIPGAVIQIQKDGRIVYSKAYGLSQKYDIRHQPLQSAPAMDTATLFDLASLTKVV
                     GTTTSLMLLADRGLIHIDDPISRYLPGFDTGEKKKITIRHLLTHTAGLDEWYPLFYRS
                     AHRDSTIRLIESLPLKYPVGKQRKYSDLGFMLLGAIIEKLSHLPLDRFEELNVFKPLG
                     MTHTTYNPLKHGRTTDIAATSLGNPYEYRMVHDTSLGFAVPGLDPRSWDGWRHYVVRG
                     EVNDGNAWYANEGISGHAGLFSTIGDLQRLVNMLLNEGLAGNKRFISISTVRSFLSPD
                     IFRNGLGWMMDPANAFMRNAPAGSFGHTGFTGTSIAVVPEYHLSVIILINRQNVGLQP
                     KGIYYNPNPIRQAIFESVMDWCK"
     gene            complement(4208..>7993)
                     /locus_tag="VGQ51_15310"
     CDS             complement(4208..>7993)
                     /locus_tag="VGQ51_15310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9038002.1"
                     /translation="NLTAFDEDAGRLYFSTDDGRLIIFQKRAETFSVHRLTTGHLYSL
                     CRSKKSDVLYAAGSNGEVIAFHLADQRSTIVRYASAEQFNTLYEDRTGAIWIEPEKTG
                     AIRFDPSSGSFQLFSFSGGESLYFIGDRYRVFEDNSGTVWVNMKGGGFGYFDPVTKKI
                     EYSLPTPDGPDYNLPPIVFGVYYDSNGILWIKTNERDLVKIVLQENNFRQSLLAKQDA
                     SPSENEIRGIFYDRQDRLWLGAKRGNLFVYRNNLLLKGLFINPPPGGLDRVYSILQDS
                     HGNVWLGTKDNGLFKATPVNRKETKYRLTHYLPDKDNPASLPCNEIYALLEDKRGVWI
                     GSYDNGLSLIVDDGHSTKFIHGGDAFRNYPKRSFQKIRHMALDDHGNLWIGTTNGLLV
                     QGVGSDKPGVYQYKTYSNTSPETERLITNDIQFIHRDEKGRMWLATSGGGFSLASGNA
                     PLQTLQFRNYTTKDGMPNDYVLACVEDKNGNFWLATENGIARFNPKTKVIRNYDSYDG
                     LPKIAYSEASVCSNASGSEIVFGTRHGYITVDPLHINTTRIEANLALTNLQINNEDVG
                     PGKNGSVLADDINYVSDLALKNDQNTISIDYGILDHRAGNRQAFAYRLVGFDTTWHND
                     RQFRRTTYTNLPPGHYIFEVKALSTDLYTNQPYRSLFITILPPWWKTAWAYAIYVIAA
                     GLLFYFIRRNAMDKIRLRNKIALEERLAALKMNFFTNVSHELRTPLTLIVNPLEQLSK
                     KGNLSSEEASWVDIARRNADRTVRFINQLLDLRKVQSDKATIRVSRVEIVDFVKKVSD
                     HFSGAVRARRINWSIVSNQQEIFAFADAEKLDVVIYNLLSNAIKFTPEGKAITVAINA
                     LPGGEYFSIAIQDQGPGVPKEQLEEIFELFREIEHPAGRELKGTGIGLALSREFVRLH
                     KGTIRAENNSDGGLTMTVELRLGAAHFEKQQIAAVVDPPMTTNVPEEPIGQQMHSLPV
                     STPQAPLISPPHGALMSPPHGASMSPAEAPLVLLVEDNDELRSFIEGQLNKHYRVVTA
                     GDGEEGLQKANDLAPDLIVSDIMMPKMDGIQLLDRIKNDENTSHIPVVLLSAKYSIES
                     QIEGLRYGADYYITKPFNNEFLIASINNLIHQRKKLFEALVGKTRSIGSIGSIGLIAE
                     PGVITSRDQSFLKQVIKVVEDKMADTDFNIDVVAESLAMSQKTFYRKFKSLTGLTPVE
                     FVRDMRMQRAKQLLEGGGNNVSEVAYHVGFSNPKYFSTCFKEKYHISPSEYARLPSH"
BASE COUNT         1909 a         1871 c         2136 g         2077 t
ORIGIN      
        1 ccccactgcg gttattgttt gaaaacggca gtggatttag ccggcttgca gccgaaacac
       61 gcgaaaggcg gggtgaacaa catgaccccg agttcccagc cggcgccgag actgccgcct
      121 atctcctggc cgtacgtaag cgcgaagacg gaatagggac tgggttccca tcgcagacct
      181 attttatatt ccgggatcgt acctgtggtg ccggcggctc cgaatatttc accgacgaca
      241 gagacagcgg gaccctttgg ataccatgcc aaccttgtag aataagtgaa agcccagccg
      301 gatatgcctt cttcgccgta attgttggta tagctggcac cgggcatgag atcccacgaa
      361 agagtattgt taaaaaacgg gatggtagag ggcacattca tccaatacgt tttgaaggcg
      421 tcttccgccc taagctcggc attcaatgtg ccgggaaaca ttcctgtgcc ggccttgacg
      481 gcgatgccgc ccgtttgcgc cttgttctcg cagaacatgt acttaaaata gagcgaataa
      541 gaataaccgt cgttggtggt ggaattgtta tcattgttat acaaataggc agccatggtg
      601 aactcccatt tgggaaaaag cgaataagtg ttcatgatca tggtattccg ctggccgata
      661 gtcggaatga gggtgacggt gccccagggt ttagatagcc agctgtcgga gttgaattgt
      721 tgtgcttcgg cattcgtgat ggccataaac gttagaaaaa gaaaatgccg gaattttgaa
      781 gtactgcaca tgccgatcgg tcgtatgtgc aagtttgggt attcggccgg aagcgccaag
      841 ggtcgaatcc atggagtggt aaggatttgt gaattttggc aagacgggtt gcccttagta
      901 cttggggtgg gaattaactt tatgccgcag gcaagagggg tcttggatcg aacagcaccc
      961 ggagttcggc gatcttttca ttaatcaggt gataccagcc gcaggtagct attgtttttt
     1021 tgcccatggc gatgtggtac caaaggaaga cgtcggtatc atcgccaacg gccttttcaa
     1081 cccggtattt gaacttcatc ttctccatgt cggtgaaata ggcttcggcg ctgtcgcgag
     1141 tgtgttttgc catgatcgat cggttaattt ctcaaatttc ggttaatagc cttattgata
     1201 tcgcaaactt cgggcctggt cgagaaaacg ggatcgttgg ctggtgttgg tgaggatgcg
     1261 gcttgtgcgg gcggctgggg cgggtggggg gcggccgatt cgtcgatggt tcaggctgat
     1321 tcgagggaaa gctcgatgta ggcttctata ccgtcttcgg agggattggc ccagaaggtc
     1381 agcacttcac tgtcgatcgg agcaaagatc tccttttcgc cgggagcgtc cggcagcgct
     1441 cttaccggga caaggataaa ctggttgagc tcgatacgat cgtttcgttg agcgggcgta
     1501 tagcctttga tcagtacgta caggaatcct ttatccttta gttcctgcag gcttttgtcg
     1561 gtgagtttta tgcgagagtg cttcctggcc atacggatgc gattgacgga gcgggtcggt
     1621 ttttttcaac cgccgccgat ttgtaaattt agaaaggatt cgggccgcgg ccggtgatca
     1681 atatcacgcc aaaaagtgat aaaagtcaat tttatgagta taaagggtct tttcccgttg
     1741 cataaatggg attttgagag cggtcgggtg ctggcggatc tgcctgagca ggacctcgca
     1801 ctgctgatga gtagaaaaac cgaaagcgca tataagaagg gacaggtcat ttttcgggaa
     1861 gggggctacc catccggact ttattacgta gtgaacggac gagtaaaaaa atataagtcg
     1921 gaccacgatg gaggcgagca gatcatctat gtcgccggta cgggcgaact gttgggtttt
     1981 catgccatac tttccggaga caattatccc gactcagctg cggtgctgag tcatgaattt
     2041 gtcgtgctgg taaacagcct cactatggcc tcgcgtaggc ctgtccggga gcgattggca
     2101 ttgcaattga tcgtcgtacg ggaaaaatac aagtcggaga cgaacaactc acccgtagag
     2161 atcaacctga gcagggacga tctggccagc ctcgtgggaa cggccaggga gaatgtagta
     2221 aggatcctgt ctgaattcaa ggaagagggc attctttcaa ccagaggaag gaagattatc
     2281 gtgcgggata tcaaaaggtt gatcgaggtg gccaattata aataacacct atcaatatat
     2341 gggaacgcct atcactaatc tcctgttgtc cataatgctg tctgttgtcg cgctttcccc
     2401 ggcggaccga ttgcaggcgc aaacaccagc gcgctataaa tcctacgaag gcgaatatcg
     2461 gtcgagagac gactcggatg atatggtccg gttgatcgcc gcggatacgg gaatagtgat
     2521 gaagcagcta tgggacagga aagagcttag gttctattcg gtgaccgata cgtatttcta
     2581 caatgccgag ttgcgttatg cgttatatat tcttaaagat gagtcgggga aggcgaaaag
     2641 tatcttgctc ggcagggccc gttttgagaa agtggggcca tagaggcgag agagaaagac
     2701 cgttcgatca tttgcaccaa tccatcaccg attcgaagat agcctggcga atgggattgg
     2761 ggttatagta aatgcctttg ggctgtagtc cgacgttttg cctgttgatg agaatgatca
     2821 cagagagatg atattccggc actactgcga tgctcgtacc cgtgaaaccg gtatggccga
     2881 aagaaccggc cggcgcgttc ctcatgaagg cgttggcagg gtccatcatc cagccaaggc
     2941 cgtttcgaaa gatgtcgggg gaaagaaagg accggactgt cgaaatggaa atgaagcgtt
     3001 tgtttcccgc gagcccttca ttcaacagca tatttaccag tcgctggaga tcgccgatgg
     3061 tggagaacag tccggcatgg cctgaaatac cttcattggc ataccaggcg tttccatcgt
     3121 tgacttcacc tctcacgaca taatgccgcc agccatccca ggatcgggga tcgaggccgg
     3181 gtacggcaaa tcccagggag gtgtcatgca ccatccggta ctcataaggg ttgccaagcg
     3241 aggtagcagc gatatcggtg gtccttccat gtttcaaagg attgtaggta gtgtgcgtca
     3301 tcccgagcgg tttgaagaca ttaagctctt cgaatctatc gaggggcagg tgggacagtt
     3361 tttcaatgat ggcgccgagt agcatgaatc ccagatcgct gtactttcgc tgtttgccga
     3421 ccggatattt caggggcagg ctttcgatca ggcgaatcgt tgaatcgcgg tgtgcggacc
     3481 tatagaaaag cggataccat tcatccagac cggccgtgtg tgtaagcaag tgacggatag
     3541 tgatcttctt tttttcaccg gtgtcgaaac ccggtaaata ccgggagatg ggatcgtcga
     3601 tatggatgag gccgcggtcg gccagcaaca tcagggatgt ggttgtaccc acgaccttgg
     3661 tcagcgaagc caggtcgaaa agggtcgcgg tatccatagc cggggcggat tggagaggct
     3721 ggtgacggat atcgtatttc tgcgacaggc cataagcttt cgaatagacg atcctgccgt
     3781 ctttttggat ttgaatgacg gcgccgggga tgaggtcggc cctgatcgcg gactgcacga
     3841 tcgagtctac cctggcgcgg agcgccgctt ccggaactgg tttggatcct tgcgcgtaaa
     3901 tcggggtcgt caggacgacg agggctgtga cgagaataaa tggttgggtc ttcatcttag
     3961 gctttggtca gccataaaga tagtgaagca gtagccatct gtgaaccttt aggagtcgct
     4021 cctaagaata agagcgactt aaaggttact acttgtatgt aaaaagagat ctatttttcc
     4081 gggtgcaaac atagagaggc gggtaacaga aagggttcag atttcggaca aaaaggttca
     4141 attttttgcc aatgccaatg tccgccattg cgcacaagcg ttttataaat tgtgataaat
     4201 ttatatgtta gtgagaaggg aggcgtgcgt attctgaagg tgaaatgtgg tatttctcct
     4261 tgaagcaggt gctgaagtat tttgggttgc taaaaccaac atgatatgct acttcggata
     4321 cgttgttgcc acctccttcg agaagctgtt ttgctctctg catgcgcata tcccgtacga
     4381 attctaccgg cgtaaggccg gtaaggctct tgaactttct ataaaatgtt ttctggctca
     4441 tggccaatga ttcagccaca acatcgatat tgaaatccgt gtcggccatt ttgtcttcca
     4501 ccactttgat cacttgtttt aaaaagcttt gatcccggga ggttatgaca ccaggctcag
     4561 cgatcaaccc gatcgacccg atcgacccga tcgatcgggt ctttcccacc agcgcttcga
     4621 ataatttttt acgctgatgg ataaggttat tgatagaagc aattaaaaac tcattattga
     4681 acggttttgt gatataatag tcggctccgt atctcaaccc ttcgatctgg ctttcgatgg
     4741 aatatttggc ggatagcaga accacgggta tatgactggt gttctcgtcg ttctttatcc
     4801 tgtccaggag ttggatgccg tccatttttg gcatcatgat gtcacttacg ataaggtcgg
     4861 gagccaggtc gtttgctttt tgtaagcctt cttctccatc ccctgccgtt actacccgat
     4921 aatgtttatt caattgtcct tcgataaagc tccggagctc gtcattatct tctacaagca
     4981 ggaccagcgg ggcctccgcg ggggacatcg acgcgccatg cgggggagac atcaacgcgc
     5041 catgcggggg agagatcaac ggtgcctgcg gggtggagac cggaagggaa tgcatctgtt
     5101 ggccgatcgg ttcttcagga acgttcgttg tcatgggcgg gtccaccacg gcggctattt
     5161 gctgcttttc gaaatgggcg gcgccaagtc tgagctctac cgtcattgtc agcccgccgt
     5221 cgctattgtt ttcggcccgg atcgtgccct tgtgaagccg gacaaattcc cgggacaatg
     5281 ccaggccgat gcccgtcccc tttaattctc gtccggcggg atgctctatt tcccggaaca
     5341 attcgaagat ctcttcgagt tgttcttttg gcacccccgg gccctggtcc tgtatagcaa
     5401 tagaaaagta ctctcctccc ggaagggcat taattgcaac tgtgatcgct ttgccttccg
     5461 gagtgaactt gatagcattg ctcaaaaggt tgtagatgac aacatccagc ttttcggcgt
     5521 ctgcaaatgc gaagatctcc tgttggttgg aaacgatgga ccagttaatc cgccgggcac
     5581 ggacggcccc ggaaaaatga tcgctcactt ttttcacgaa atcgactatc tcaaccctgg
     5641 aaacgcgaat ggtggctttg tcgctctgga ccttccgcaa atccagaagc tggttaataa
     5701 aacgaacggt gcgatccgca ttccttctcg caatatccac ccatgatgct tcttcggaag
     5761 aaagattgcc ttttttggac aactgctcca gaggattaac gatcaaggtg aggggcgttc
     5821 gcagctcgtg cgaaacattg gtgaagaaat tcattttcaa tgccgccagc ctttcctcca
     5881 gcgcgatctt gttgcgcaac cggatcttgt ccattgcgtt gcgcctgata aaataaaaca
     5941 atagtcccgc agcgatcaca tatatggcat aggcccacgc cgttttccac catggaggca
     6001 ggatggtgat gaataaactt ctatagggtt ggttggtata aaggtcagtg ctgagcgctt
     6061 ttacctcgaa gatataatgt cccggcggca ggttggtata ggtggtccgt ctgaactgcc
     6121 tgtcgttgtg ccaggttgtg tcaaaaccaa ccagccggta agcgaaggcc tgcctgttgc
     6181 cggcccggtg atcgaggatc ccatagtcta tgctgatcgt gttttgatcg ttttttaacg
     6241 caagatcgga aacataattt atgtcgtcgg ccaggacaga tccattcttt cccggaccga
     6301 cgtcctcatt attgatctgt aaattcgtca gggcgagatt ggcttcgatc cgagtcgtat
     6361 taatatgcag gggatcgacc gtgatatagc catgtcgggt gccaaagacg atttcacttc
     6421 ctgaagcgtt ggagcagacg gaggcctcgg aatatgctat tttaggcaag ccatcatagg
     6481 aatcatagtt gcgtattact ttggttttgg ggttgaacct ggcgatgccg ttttccgtgg
     6541 caagccaaaa attaccattc ttatcctcca cacaagccag cacataatca ttcggcattc
     6601 cgtccttcgt tgtgtaattt cgaaactgta aggtttgcaa cggcgcatta ccgctggcaa
     6661 ggctgaagcc gcctccggag gtagccaacc acattcttcc tttttcatcc cgatggataa
     6721 actggatatc attcgtgatc aacctttccg tttcggggga tgtgttacta taggtcttgt
     6781 attggtaaac tcccggtttg tcgctgccga ccccctggac taacaatccg tttgtggtcc
     6841 cgatccacag gttcccatga tcatccaggg ccatatgcct gatcttttga aaagagcgtt
     6901 tcgggtagtt ccggaatgca tcgccgccat ggataaactt tgtgctgtgc ccgtcatcta
     6961 caatcaatga gaggccattg tcataagacc cgatccatac cccccgttta tcttccaaca
     7021 atgcatagat ctcgttgcaa ggcaaacttg cgggattgtc tttatccggc aaataatggg
     7081 tgagcctgta tttggtttct ttcctgttga caggagtggc cttgaataaa ccattatcct
     7141 tggtcccaag ccatacattc ccgtggctgt cctgaagtat cgaatagacc ctgtccaatc
     7201 cgcccggcgg cgggttaata aacaacccct ttaacagcag gttgttccgg taaacaaaca
     7261 gattgcccct cttcgctcct aaccacaggc gatcttgtct atcgtaaaag atgcccctta
     7321 tttcattctc agaaggcgag gcgtcttgtt ttgccaacag cgactgtcta aagttgtttt
     7381 cctgcaacac tattttgact aaatcccttt cgttcgtctt tatccagagt atcccattac
     7441 tgtcgtaata aacaccgaac acaatcggtg gaagattgta atccgggcca tcgggtgtag
     7501 gcagtgaata ttctattttc ttcgtaacgg gatcaaaata accaaaacca cctcccttca
     7561 tattgaccca gacagtgccg ctgttgtcct caaaaacgcg ataacggtcg ccgataaagt
     7621 aaaggctttc accaccacta aatgaaaaca gctggaacga cccggaagaa ggatcgaaac
     7681 gaatggcccc tgtcttttca ggttcgatcc aaatggcacc tgtccggtct tcatacaaag
     7741 tattgaattg ttccgctgaa gcgtatctta ctattgttga tctttgatcg gccagatgaa
     7801 aagcgatcac ttcaccattc gacccggctg cataaagaac atcgcttttc ttcgaccggc
     7861 agagggaata cagatggccg gttgtaagcc tgtgaacgga aaatgtctcc gctctttttt
     7921 gaaatatgat aagccggcca tcatcggtgc tgaaataaag ccgcccggca tcttcatcga
     7981 atgcagtgag gtt
//
LOCUS       DASYSV010000497        21398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2279008, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000497 DASYSV010000000
VERSION     DASYSV010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 21398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21398
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2279008"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..2835
                     /gene="clpB"
                     /locus_tag="VGQ51_15315"
     CDS             193..2835
                     /gene="clpB"
                     /locus_tag="VGQ51_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEV9038003.1"
                     /translation="MNLNNFTIKAQEAVARSQQLAFESQNPAIDTDHLLKALLAEEDS
                     PVGFLLKKNNVNVAFVESKLEESIQKLPKVSGGEPAQTISRELNNAVLRAGSDLKQFG
                     DEFVSVEHLLLGMVQGSDNTAKLLKDSGLTEKGLVAAIKDLRKGSTVNSQTQEATFNA
                     LNKYAKNLNELARQGKLDPVIGRDEEIRRTLHILSRRSKNNPILVGEPGVGKTAIAEG
                     LAHRIVNGDVPENLRSKTIYALDMGQLIAGAKYKGEFEERLKSVVKEVATSDGEIILF
                     IDEIHTLVGAGGGEGAMDAANILKPALARGELRAIGATTLNEYQKFFEKDKALERRFQ
                     RVMIDEPSVDDAISILRGLKDRYETHHHVRIKDEAIIAAVELSHRYITDRFLPDKAID
                     LIDESAAKLRLEMNSMPEELDKLERQIRQLEIEREAIKRERDEDKLRELSAEIGNLSV
                     SRDTLKAKWKQEKELVERVQNAKAEIENLKQSAEKAEREGQYGQVAEIRYGKVKEQER
                     IIEEYSGQLAKMNSGTRLMKEEVDAEDIAEAVAKTTGIPVTKMLQSEKEKLLNLEDEL
                     HKRVVGQDEAIEAVADAIRRSRAGLQDPRKPIGSFIFLGTTGVGKTELAKALAEYLFD
                     DETMMTRIDMSEYQEKHTVSRLIGAPPGYIGYDEGGQLTEAVRRKPYSVVLLDEIEKA
                     HPDVFNILLQVLDDGRLTDNKGRVVNFKNTIIIMTSNMGSQIIQANFEAVTEKNRETV
                     MERTKVEVMDVLRQSIRPEFLNRIDEIILFQPLLKGEIRGIIRIQLENLQRLVAKNGV
                     NLQFSDYAIDYLAENGFDPQFGARPLKRLIQKEIVNQLSKKILRGDIDRTSPVLVDVF
                     DGVVVFRNEHPQVEKSDRMAEPKR"
     gene            2855..3403
                     /locus_tag="VGQ51_15320"
     CDS             2855..3403
                     /locus_tag="VGQ51_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEV9038004.1"
                     /translation="MKAKESIEIIPTKNVFVGDPKAPVTLMEYGDYESEACARVNEVV
                     DRLLKEFPGKVRFNFRHFPLTKIHQHAMKAAEASLGAAQEGKFWEMHSMLFEHRRRLG
                     AISLREYARDVGVVNKKFLDHLVNSTYGWQVRSDLLEGLNQGVRDVPAFFINGVLFEG
                     KPTFDNLRKGIEAALKQKKKRA"
     gene            3452..3979
                     /locus_tag="VGQ51_15325"
     CDS             3452..3979
                     /locus_tag="VGQ51_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEV9038005.1"
                     /translation="MVKFKTKILQFQQMGEKTGWSYIRVPAALAQQLKPGNKKSFRVK
                     GRLDEYPISGMALIPMGEGDFIMALKAATRKAIRKQKGDELEVQIEVDKKEIAPPKEL
                     IECLADEPEAQRYFRELPKSHQNWYGNWVREARTEGTRARRIAQIVEAMVKRLSFQDM
                     LRAYGANARAMREDR"
     gene            4139..4648
                     /locus_tag="VGQ51_15330"
     CDS             4139..4648
                     /locus_tag="VGQ51_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038006.1"
                     /translation="MDLSHSKRISTAGLLITLGIIYGDIERTDEPYTMEYGVEGLMND
                     KVIRVEFRLGFRVQQRVGMMFRKVVMEMISNKELDVVSRFPSLKKHNIAEDFRFVILE
                     KFLSYDNEFSVREGFILNTYFSMKKLALAEEKAFGLDSSETVVEKLPLVVPPASKINL
                     QRAYYRMHR"
     gene            complement(4679..6088)
                     /locus_tag="VGQ51_15335"
     CDS             complement(4679..6088)
                     /locus_tag="VGQ51_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEV9038007.1"
                     /translation="MKTIVFALAGLVTCAAAHAQPAAKEMSWYIHNAPFKMPDIAQPG
                     FPDKTFRITDYGAIGDGHTLCTDAIRKTIAACSAAGGGHVIIPGGNWLTGPIELLSNV
                     DLHLEKDALVQFTSDHSAYPALGPRPDAVMPPLYAHDQENIALTGEGEMDGAGQTWRP
                     VKKEKQTSEQWRQLLSSGGVLSADGKIWWPDKEDMEHPNRRPVMVSFVKCKRIMIQGV
                     LLRNSPKFVFYPGHCTDLTLDHVRIYNDWWAQNGDGIDISACKRVMIYGCTVNAGDDG
                     ICMKSSGASPDGPALEDVVIAGCTVLRAHGGFVIGSNTDGGMHDIFVSDCAFSGTDVG
                     LRFKSNMGRGGLVNDIYIQDIAMQNIAKEAVLFDTYYEDAPAGVTQTHESHTDKTPEF
                     RDFHISRITCKGAKTAISITGLPQMPVSDIRFDKVSITAEKGLIATQCRNIDLDHVTL
                     DVSEKPAIRTDATADVRVH"
     gene            complement(6157..7095)
                     /locus_tag="VGQ51_15340"
     CDS             complement(6157..7095)
                     /locus_tag="VGQ51_15340"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV9038008.1"
                     /translation="MSTHTILGAGGAIANSLSGELTARHIPLRLVSRHPSPMTGAEAV
                     SADLTDRDQTIRAIEGSSVVYLCVGLKYDYRIWRQQWPRIFDNTIEACMKTKTRLIFF
                     DNVYMYGLTRGPMTEETPYDPSSRKGDLRARLATQLMSEVRKGNITASIARAADFYGP
                     ATAKTSVPDFLVFQRLLKGQRAQWLVNAKVPHSFTYVPDAARALAILATDDNSWNQVW
                     HLPTTAPAITGAEFIRIADTLLQKDPRFSILSSWMVRLGGLFDRTTAELHEMLYQYAY
                     DYHFDSSKFERAYNFKPTSYENGIKATIDWHLRAGA"
     gene            7349..8671
                     /locus_tag="VGQ51_15345"
     CDS             7349..8671
                     /locus_tag="VGQ51_15345"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9038009.1"
                     /translation="MKKVFQRLFISSGFSFICLIAGAQKAPDTLTDATVQGCVQYALK
                     HYPQIQQALLDEQITERQIRSHLADWYPQIGLTGSFQHYFQLPTVAFNGGIVASGVPN
                     NSTVALGLNQTLFNRDVLLAERTKGDVMTSIRQTTVSDKISLAVNVSKAFYDVLLTAK
                     QIDVLDDDIVRLERNLKDAYNQYRGGIVDKTDYKRATISLNNSKAERKNDEEALKAKY
                     ALLKQLMGYTPADSLHLQYDSAQMEASIYLDTLAPVDYSKRIEYQQLMTGRRLQQADV
                     KYNKWAYLPSVTANAGYNMNYFNHSLDNLYGNNFPSSYAGLQVNVPIFQGTKRTQNIR
                     IAELQLTRINWDVVNLQTAISTEYAQAMASYKGYLNNYFVERENLGLAQEVYNTIKLQ
                     YNSGIKAYLDLITAETDLRTAELVYSNALFQVLSSKLDVEKALGTVQY"
     gene            8721..9839
                     /locus_tag="VGQ51_15350"
     CDS             8721..9839
                     /locus_tag="VGQ51_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9038010.1"
                     /translation="MRSRLQGMGLLSMATALCLASCGNKPTGPQAPPPTAVNVYTVEA
                     GNATYFDNYPATATPLNQVDIKPQVSGNIVGIFFKDGQRVRKGQKLYEIDQLQYKAAV
                     EQAKANLDVAKANLARSQQDYDRYQELAKQDAIARQVLEHQVADLEANKRSVQAAQAN
                     VASVETNLKYSEIYAPFDGTIGISLVKVGTSVYPQTLLNSVSTDDPMAVDISIDQAEI
                     PRFTTLLEKGRNKDSTFTAVLPDGSIYPHPGDLYLIDRSVDPTTGTIKVRLTFPNPKD
                     ELKAGLTTNIRVKHSSGDSTLLIPFKGVVEQLGEYFVFVADSGHAYQQKVTLGPRIGG
                     MVVARSGLKAGEKIVVDGVQKLRDSSAIKAAPVKGIVK"
     gene            9862..13029
                     /locus_tag="VGQ51_15355"
     CDS             9862..13029
                     /locus_tag="VGQ51_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9038011.1"
                     /translation="MKSSNVFLRRPVTAIVISLVILLVGGLAILNMPVSQYPDITPPT
                     VQITANFVGADAQTVEQTTTTSIETQVNGVPGIAYMQSNSANSGTASITATLNIGANP
                     DIVALDIQNRVGIALPTLPTQVQRLGVTTLKRNPSILMLVAIFSPKGTHSVPFMDNYA
                     NIYLRDILLRVKGVGDVISRADNFSMRVWLKTDKLAQLGLTANDVQNAINEQNLQIAA
                     GSVAAPPQNSNAAFEFSVFTNSRLNTEEQFGNIVIRSNPQQNSIVYLKDIARIQLGKV
                     SYAANSFVDGKRASYLLIYQAPGSNALETADGVYKAMADAKKYFPADVAYNVPFESVS
                     VVKVSIEDVVRTLFIALALVTFVVFIFLQNWRATLIPVLAIPVSIVGTFIFFVPLGFT
                     INTLTMFGFVLAIGIVVDDAIVVVEAVQHYIDVEHLSPKEATARAIADISGPVIAIAL
                     ILAAVFVPVGFIPGIVGRLYQQFAITIAISVLISAFVALSLTPALCMLLLRPHKVDKK
                     ARGLSWVFYKFNQGFHRFTMHYTIGVKGWIKYAPLVLVLLACIIAGDIFLFKIKPTGF
                     IPLEDEGRVYISFEIPEAGSTTRALTVLDSVQHIAQGTPGVLHTAALGGLNILTFGFK
                     SNAGSIFMQLKPWDQRKSKAEQEKGVIAELNKRFAAIKDARIIVIEPPAIPGLGNAGG
                     FSIQIEQRQSNDDIKTFERVVNQFVAEANKQPEIGQAFTFFGAHTPGYEVDVDRDKAK
                     KLGLNLADVYNTIQMYMGSTYINQLTLYGRNYYVVAQADTGYRTEIHAIDHYYVKNMN
                     GQMIPLSSIVSYKLTETAPLISHFNIYRSAEIDGGAKPGFSSGQAIQALERVAAKVLP
                     EGYGYDWSGLSLQEIKSGNSSVYIFMLSVVFVFLFLAALYESWSVPFSVLLAVPIGVF
                     GAIFTLWLIPRLTNNVYAQIGLITLIGLSAKNAILIVEFAKERVDRGMAVVSATLDAV
                     KLRLRPILMTSVAFLLGVLPLALASGASSVARNTIGFTVLGGMFAATSLAIFVVPVLF
                     VVITRLTYGKRKLDELRHQQLNLAE"
     gene            complement(13071..15494)
                     /locus_tag="VGQ51_15360"
     CDS             complement(13071..15494)
                     /locus_tag="VGQ51_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038012.1"
                     /translation="MLGNFFRFTLRHFQRNKWFTVINIAGLALGIAACLVILLYVADE
                     LSYDRYNEKADRIVRVFFRGVMEGGEIKEANVMPPVAQALKSDFPEVLAATRIRTYGI
                     PRLVVGSKIFREDKFAFVDPNFFQVFTLPLIRGDAKTALDRPNTIVITRTVAERLFGK
                     EDPIGKTLISKDGGLKPMIVTGEIEDVPANSHFHFGLFASMTALPEAGISSWMSSNFF
                     TYLVLPKGYDYRKLEAKLPAEIDKYIGPQLKQGLGISLEEFRKKGNSIGLFLQPLTDI
                     HLHSDLTGELEPNGNIDYVYIFSAVAIFMLLIACINFMNLSTAAATRRAREVGVRKVL
                     GSLRGQLIRRFLGESLLLTASAFLLAIGLVYLALPAFNDIAGRNLSFHWDSNPGLTIL
                     LIALVLFTGLLSGSYPAFFLSAFRPIAVLKGRLSSGGKSMTLRSGLVVFQFFISISLI
                     VGTAIVYDQLSYIHHKKLGYDKDQVIVLQEPYWLGNNFEAYRQRLLQDPRIASITTSG
                     YLPAGPSYSNNFMAYGDGQSTQLVKSVRFDVDYDYIPTLDMEMATGRNFSRSFGADSS
                     AMILNQAAATAYGWGENAVGHTVTHSEGDGVSRTYHVIGVVKDFNFRSLHQLITPLVM
                     TLGGDESNLIVKARTKDLPGLLATMQNEWKNAKAEMPFSYSFLDDRFNNTYKSEQNIG
                     RILGIFAGLTIFVACLGLFGLATFTAAERTREIGIRKVLGAGTSSIVTLLSKDFLRLV
                     ALAFLIAAPVAWLVMNKWLQDFAYRITIPWWIFAITGVLVTLITLLTIGFRALKAASA
                     NPVDSLHTE"
     gene            15885..16535
                     /locus_tag="VGQ51_15365"
     CDS             15885..16535
                     /locus_tag="VGQ51_15365"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HEV9038013.1"
                     /translation="MNLIIDIQYFAPFSYYKISYKSSNIIFEQYETWQKLSFRNRCRI
                     AGAEGVIDLSIPLSGGRDQKTVMKEVRIADGKGWQDRHWKTIVSCYSRSPWFDHYRDE
                     LEGLYRRPANFLLDWNLLCFEWSMKALGMSRPVQLSQEYRKTYSPEEGLDWRGRIVPG
                     AAGEVPGAGKAPVPGAAGEVPKYRQVFEERIGFIPDLSILDVLFCEGREAIGYVLA"
     gene            complement(16612..18342)
                     /locus_tag="VGQ51_15370"
     CDS             complement(16612..18342)
                     /locus_tag="VGQ51_15370"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEV9038014.1"
                     /translation="MSLLWAIYHLFVLIFRDASARMRHGLAAMLLTIGAAWTILTFIT
                     TYWLGTADSPYWLGPATGAYLLPASLQPYGNRLLSVSRWFIDEGLSWCSFGYLLVLGG
                     LLIHYTGHYLRSRRITKMGLSKIGPGFRIFVAETARNMGILPVVKVHLSSLVDVPVTL
                     GFLRPMILLPVAMITHLTTQQIEAILVHELAHIRRKDYLINILVTVMELVFFFNPFAR
                     MLIGQLKKEREHCCDDLVLQFQYDAHAYVSALLSLARQHRHGRLAVAATGGDNKLLLQ
                     RAKKILQHHRSDDRPGARPLFLLFLTIGMTLLFWGPRRMTPPLPPASVAMMRPAGTAH
                     PYIETDKRLIVNIASSNVKSLPPTNLSIAHLPPTHIATRAIAHASAPSATHHGAASPV
                     SHRRATHPPAAGAFNEDEATWFRTSGPSNTPAPAYADLVVIDSRDYSIGSPSGNGQTA
                     VAPPTASEGFPFVPQSSFSFHYTDTLPPEDRLAIMEKVTEKAIRLQISQLREELKTRL
                     NALQAAQAALDKASRNPTLSTDLQSASRKQLKQLLKDQLLLQQRYMLRLDDLQRQLKQ
                     AARRLTTVYI"
     gene            complement(18412..18792)
                     /locus_tag="VGQ51_15375"
     CDS             complement(18412..18792)
                     /locus_tag="VGQ51_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEV9038015.1"
                     /translation="MPTKTIKPTESELEILQVLWERDQASVRDVHEELLKCKDVGYTT
                     TLKLMQIMFDKGLVKRDDSFKTHIYQASVSKEKTQKHLLGKMINTLFGGSPGELVLQA
                     LGNHKASPAELEEIQLLLNNLKEQ"
     gene            complement(19048..21267)
                     /locus_tag="VGQ51_15380"
     CDS             complement(19048..21267)
                     /locus_tag="VGQ51_15380"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEV9038016.1"
                     /translation="MDNLLYLVPVLGAVGLVYTFIKFSWVSKQDPGNARMQEIGQHIA
                     QGAMAFLKAEWKILSYFVILAAILLAFMGYTNPHSHWSIAISFILGAVLSATAGYIGM
                     RSATKSNVRTAHAARTSLAQALKVSFTGGSVMGLGVAGLAVIGLGGLFILLRAIFASG
                     APVNSDEMRLTIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLFGSYVATVLATMVLGQETISDDNFGGLSPILLP
                     MLIAGIGIIFSIVGTLFVKISDSSAVNTSTVQKALNMGNWGSIVMTAIACIFLVYYIM
                     PDTMTLRGTEFTKWGVLGAIGVGLLVGTLMSIITEYYTAMGKAPVLSIIRQSGTGHAT
                     NVIGGLAVGMQSTFLPILVLAGGIWGSFACAGLYGVAIAAAGMMATTAMQLAIDAFGP
                     IADNAGGIAEMSELPKDVREKTDVLDAVGNTTAATGKGFAIASAALTALALFAAFVGV
                     AGIQGIDIYKAKVLASLFVGGMIPFIFSSLAIQAVGQAAMAMVEEVRRQFRTIPGIME
                     GTGKPEYDKCVAISTQASIRKMVLPGAIAILFPLIIGFILGPEALGGFLAGATVCGVL
                     MGIFQNNAGGAWDNAKKSFEKGVEINGEIHYKKSDPHKASVTGDTVGDPFKDTSGPSM
                     NILIKLMSIVSLVIAPTLASIFHTRDTGLAGQKHPVEKKVAAAQVKAPAPGTVAAYR"
BASE COUNT         5058 a         5132 c         6470 g         4738 t
ORIGIN      
        1 ctgaatcgtc ggcagtttag ttgggcatta tatgacataa tagctggatt gaggtccgtg
       61 gtttggattt tgtgacacaa gtttactatt tattcaattt cggcaaaaaa tgataaaaaa
      121 gtaatttttt gcctcaatac tcaaatgtct ttgaattgga ctccgtccaa ttcggaggaa
      181 aatcataaag atatgaacct gaacaatttc accataaaag cacaggaggc cgtagcccgg
      241 tcgcagcaac ttgcgttcga gagccagaac cccgccatag atacggatca tctgctgaag
      301 gcgttgctgg cggaagaaga ttcaccggtc gggttcctgc tgaaaaagaa caacgtgaac
      361 gtggcctttg tggagagcaa gcttgaggaa agcatccaaa agctgccgaa ggtgagtggg
      421 ggagagccgg cgcagaccat cagccgcgag ctgaacaatg ccgtcctgcg ggcgggatcc
      481 gatctgaagc agtttgggga cgagttcgtg agtgtcgagc atttgctgct gggtatggta
      541 caggggagcg ataatacggc caagctgctg aaggattcag gtctgacgga gaagggactg
      601 gtggccgcga tcaaggatct gcggaaaggg tcgacggtta acagccagac gcaggaggcg
      661 accttcaatg cgctgaataa atatgcaaag aacctgaacg agctggcccg gcagggcaaa
      721 ctggatcccg tgatcggacg ggatgaagag atccgacgga ccctccatat cctgtcgcgc
      781 cggagtaaga ataacccgat actcgtgggt gaacccgggg tgggtaagac ggccattgcc
      841 gaaggtctcg cacatcgtat cgtcaacggc gatgtacccg agaacctgcg gtccaagacc
      901 atttatgccc tcgatatggg gcagctgatc gcgggcgcca aatataaggg tgagttcgaa
      961 gagagattga aaagtgtcgt aaaagaggta gctaccagcg acggtgagat catcttgttc
     1021 attgatgaaa ttcatacctt ggtcggggcc ggcggaggcg aaggggccat ggatgccgcc
     1081 aatatattga agccggcgtt ggcgagaggc gagttgaggg caatcggggc tacgacattg
     1141 aatgagtatc agaaattctt tgagaaggat aaggcgctgg aacggcggtt ccagcgagta
     1201 atgatcgatg agccgagtgt ggatgatgcg atctctatcc tgcgtggttt gaaagaccgt
     1261 tatgaaacgc accaccatgt ccggataaag gacgaagcta tcatcgctgc ggtggaattg
     1321 tcgcaccggt atatcacgga tcgttttctg ccggataagg ccatcgatct gatcgatgaa
     1381 agtgccgcaa agctgcggct agagatgaat tccatgccgg aagagctgga taagctggaa
     1441 cggcagatcc gtcagctgga gatagagcgt gaagccataa agcgcgagcg ggatgaggac
     1501 aagctgcggg aattaagtgc cgagatcggc aatctcagcg tatccaggga caccctgaag
     1561 gccaaatgga aacaggagaa ggaactggtg gagagggttc agaatgccaa ggccgagatc
     1621 gaaaatttaa aacagtcggc cgagaaagcc gagcgggaag gacaatacgg gcaagtagcg
     1681 gaaataaggt atggcaaggt aaaagagcag gagaggatca tcgaggaata ttccgggcag
     1741 ctggctaaaa tgaattcggg tacgcgtctg atgaaggaag aggtcgatgc agaggatatc
     1801 gcggaagctg tggcgaaaac gacggggata cccgtgacga agatgctgca gagcgagaag
     1861 gagaaactgc tgaacctgga agacgaattg cataaacgtg tcgtgggtca ggatgaggca
     1921 atcgaggccg tagcggatgc gatacgccgg agccgggcgg gtcttcagga tccgagaaag
     1981 cccatcgggt cgttcatttt cctgggcaca acgggagtcg gtaagaccga gctggctaag
     2041 gcactggctg aatatctttt cgacgacgaa accatgatga cgcggatcga tatgagcgaa
     2101 taccaggaga aacatacggt aagccggctg ataggagcac ctccgggata tataggatat
     2161 gatgaaggcg ggcaactgac ggaggccgtc cggcgtaaac cttacagtgt ggttctgttg
     2221 gatgagattg aaaaggcgca tccggacgta ttcaacattc tgttgcaggt gctggatgac
     2281 gggcggctca cggacaataa gggccgggtg gtgaatttca agaatacgat catcataatg
     2341 acgagcaata tgggcagcca gatcatccag gccaatttcg aggccgtgac ggaaaagaac
     2401 cgggagaccg tgatggagcg gacgaaagtg gaggttatgg acgtgctgag acagagtata
     2461 cgccccgaat tcctgaaccg gatcgacgag atcatcctgt ttcagccctt gctcaagggt
     2521 gagatcaggg ggatcatccg gatacaactt gagaatttgc aacggttggt ggcgaaaaat
     2581 ggtgtaaact tgcagttcag cgactatgcc atcgattacc tggcggaaaa cggtttcgac
     2641 ccgcagttcg gggcccggcc gttgaaacgc ctgatccaga aagagattgt aaatcaattg
     2701 agtaagaaga ttttacgggg tgatatcgac cgaacctccc ctgtattggt ggacgtattc
     2761 gacggggtag tggttttccg gaatgagcat ccgcaggtgg aaaaaagtga cagaatggcc
     2821 gaaccaaaaa gatagtcaac tgttattaaa agagatgaaa gcaaaagaat cgatcgagat
     2881 cattcctacg aagaatgttt tcgtgggaga tcctaaagcc ccggtaacct tgatggaata
     2941 cggggattac gaaagcgaag cctgtgccag ggtgaatgaa gtggtggaca ggctgttaaa
     3001 ggaatttccc gggaaggtcc ggttcaattt ccggcatttt cccctgacga agatccatca
     3061 gcatgccatg aaggcagcgg aggcaagtct gggggcggcg caggaaggaa agttctggga
     3121 gatgcacagc atgctattcg agcatcgccg ccggctgggg gcgatcagtc tccgggagta
     3181 tgctcgtgat gtgggagttg tgaacaagaa gttcctggac cacctggtca actctaccta
     3241 cgggtggcag gtccggagcg atctgctcga aggattgaac cagggcgtaa gggatgtacc
     3301 cgcctttttc atcaacggcg ttctctttga gggaaagccg acgttcgaca atctccgaaa
     3361 ggggattgag gcggcgctaa agcaaaaaaa gaaaagagca taagcaatag aaaggggcct
     3421 gagggcccct ttttttgtgg taaattcggg tgtggttaag ttcaagacca aaatactgca
     3481 attccagcag atgggtgaaa agaccgggtg gagctatatc cgggtgccgg ctgcgcttgc
     3541 gcagcaactc aaacccggga ataaaaagtc attccgggtg aaggggcgtc tggacgaata
     3601 cccgatcagc ggcatggcgc tgatcccgat gggggaaggg gattttatca tggctttgaa
     3661 agcggccacc cgcaaggcga tccgcaagca gaagggggat gaactggaag tgcagataga
     3721 ggtggataaa aaagagatcg caccgcctaa ggaattgatc gagtgtctgg ccgacgagcc
     3781 ggaggcgcag cggtatttca gggagctgcc aaaatcacat cagaactggt atggcaattg
     3841 ggtcagggag gccaggacgg aaggcacgag ggccaggcgg atcgcgcaga tcgtggaggc
     3901 gatggtcaaa cgcctgtcat tccaggatat gctgcgggca tatggggcga atgcgcgagc
     3961 gatgcgcgag gatcggtaag acataccaaa acggtaaaat tttttcattt tgaaaatgtt
     4021 cttagtgcgg tagactgact tgtctactga tgacaatagc ttgatttgta agggcttttg
     4081 gcgcgatggc gtcttgattg tgtataaggt acactgtttg caaaaaaagg gccctatcat
     4141 ggatctaagc cattccaaac gcatctctac ggcgggtttg ctcattaccc tcggtataat
     4201 ttacggtgac atcgagcgga ccgacgaacc gtatacgatg gaatacggcg tggaagggct
     4261 gatgaacgac aaggtcatcc gggtggagtt ccgtctcggt ttccgcgttc agcagcgggt
     4321 agggatgatg ttcagaaagg tggttatgga aatgatatcg aataaggagc tggacgttgt
     4381 cagccggttc ccttcgttga agaagcataa tattgcagag gatttccggt tcgtcattct
     4441 ggaaaaattc ctctcgtacg ataacgaatt ctcggtacgc gaagggttta tcctaaatac
     4501 ttatttcagc atgaagaagc tggcgctcgc cgaagaaaag gcgttcggtc tcgattcgag
     4561 cgagacggtg gtcgaaaaat tacccctcgt agtgccgccc gcatcgaaga tcaatctgca
     4621 acgcgcgtat taccggatgc atcgttaggg gagcatggcc gcgttatcct gcgcatcgtt
     4681 agtgcaccct gacatcggcg gtcgcatcgg tccggatcgc gggtttttcg gaaacgtcca
     4741 gtgtgacatg gtccagatcg atgttgcggc actgtgttgc gatcagtcct ttttcggccg
     4801 tgatggatac tttgtcgaac cggatatcgc ttacgggcat ttggggcagg ccggtgatgg
     4861 agatggcggt ctttgcgcct ttacaggtga tccggctgat atggaagtcg cggaattcgg
     4921 gtgtcttgtc cgtatgagat tcgtgcgtct gagtaacgcc tgccggtgcg tcctcataat
     4981 acgtgtcgaa caacacggct tctttggcga tattttgcat cgcgatgtcc tggatataaa
     5041 tatcgttgac gagtccgcct cttcccatat tgcttttaaa gcgaaggccg acatcggtgc
     5101 cggagaacgc gcagtcgctg acgaagatat cgtgcatgcc gccgtcggtg ttgctgccga
     5161 tgacgaaacc gccgtgcgcg cgcaggacgg tgcagccggc tatgacgacg tcttccaggg
     5221 cgggtccgtc gggactggct ccgctggatt tcatgcagat accgtcatcc ccggcattga
     5281 ccgtgcaacc atagatcata acgcgtttgc aggcgctgat atcgatgcca tccccgttct
     5341 gcgcccacca gtcgttgtag atccttacat ggtccagcgt cagatccgta cagtggccgg
     5401 ggtagaaaac gaacttcgga gaattccgca gcaggacgcc ttgtatcatt atccgtttgc
     5461 atttgacgaa ggagaccatc actggccggc ggttggggtg ttccatatcc tctttgtcgg
     5521 gccaccagat cttgccatcg gcgctgagga ctccgccgga agacagtagc tggcgccatt
     5581 gttcggaagt ttgtttttcc ttttttaccg ggcgccaggt ctggcctgcc ccgtccattt
     5641 ctccttcgcc ggtgagggcg atattctcct gatcatgcgc gtagagggga ggcatgacgg
     5701 cgtccggccg gggccccaat gcaggataag cggagtggtc ggaagtaaat tggacgagcg
     5761 cgtctttttc gagatgcaga tcgacattgc tcagcagctc gatcggaccg gtgagccagt
     5821 ttccgcccgg gatgatgaca tgcccgccgc cggccgcgga acaggcggcg atggtcttcc
     5881 ggatggcgtc ggtgcataac gtgtgtccgt caccgatagc gccgtagtcg gtgatacgga
     5941 atgttttgtc agggaaaccg ggttgagcga tgtccggcat tttaaagggg gcgttatgga
     6001 tataccagga catttctttt gcggcaggct gggcatgtgc ggccgcgcag gtgaccaggc
     6061 ctgccagcgc gaagacgatc gttttcatgg ctcccaaggt agacgattgg cgtcatcgct
     6121 tgaaaattag ttacgggaac gttcccggag cctgggttac gcccctgcgc gcagatgcca
     6181 gtcgatggtc gcctttatgc cgttttcata cgaggtaggc ttgaaattat atgcgcgttc
     6241 gaatttcgac gaatcgaaat ggtagtcgta tgcgtattga tagagcattt catgcagctc
     6301 ggccgtcgtc cggtcgaaca aaccgcccag ccggaccatc caggaggaca gtatcgagaa
     6361 gcggggatct ttttgcagga gggtgtccgc gatgcggatg aattccgctc cggtgatggc
     6421 gggggccgta gtcggcaggt gccatacctg gttccaggaa ttgtcgtcgg tcgcgagtat
     6481 ggccagtgcg cgggctgcat cggggacata ggtaaaggaa tgcggcactt tcgcattgac
     6541 cagccattgc gcgcgttgtc ctttcaaaag ccgctggaaa acgagaaagt cggggacgct
     6601 ggttttggct gtagccggtc cgtagaaatc cgccgcccgc gcgatgctgg cggtgatatt
     6661 tcccttgcgc acctcgctca tcaattgtgt agccagtctt gcccggagat cgcctttgcg
     6721 gctggatggg tcgtagggag tctcctcggt catcggcccc cgggtgagcc cgtacatgta
     6781 gacattgtcg aaaaatatca accgtgtctt tgttttcata caggcctcta tcgtgttgtc
     6841 gaaaatgcgt ggccattgtt gtcgccagat gcgatagtcg tatttcaaac caacgcagag
     6901 gtataccacc gacgagcctt cgattgcccg gatggtttga tcgcgatccg tcaggtcggc
     6961 cgatacggcc tccgcgccgg tcatggggct tgggtgtcgg gatacgagcc ggaggggtat
     7021 atggcgggcc gttagctccc cggaaagcga gtttgcgata gcgccgcctg caccgaggat
     7081 agtatgggtg gacataaatg cctggtttta ttggcgtaaa ggtaaggttt agcgtcctct
     7141 tcttaaaaat tgattaaacg tttgtttaat tcaaacgttt gtttaacttt gtgcccttta
     7201 aaaccttgtc ggggtaccat tcgggaagga tggggaggtg tcgcgaacga attggccgat
     7261 acggatattt atttcagcgg cggcggatat accggtcatt ctaacgattt ttgccgtccc
     7321 tgaatgggag gtaaacagca tccatattat gaagaaagtg ttccagagat tattcatcag
     7381 cagtggattt agctttatct gtttaatcgc cggagcgcag aaggcgcccg acacgctgac
     7441 cgatgcgacg gtgcagggct gcgtgcaata tgcgttgaaa cattatcctc agatccagca
     7501 ggccttgctg gacgagcaga tcaccgagcg acagatcagg agtcatctgg ccgactggta
     7561 tccccagatc ggtctcacag gtagtttcca gcattatttt cagctgccga ccgtagcgtt
     7621 caacggaggg atcgttgcaa gcggggtccc aaataattcc acagtagcat tagggcttaa
     7681 ccagacattg ttcaacaggg acgtattgct ggccgagagg acgaaaggcg atgtcatgac
     7741 gagcatccgg cagacaacgg tcagcgacaa gatcagcttg gccgtgaatg tcagcaaggc
     7801 tttttacgat gtgctcctca ccgccaaaca gatcgacgtg ctggatgacg atatcgtacg
     7861 gctggagcga aacctgaagg atgcatataa ccaataccgc ggcggtattg tagacaagac
     7921 cgattacaaa cgggctacga tctccctgaa caattccaaa gccgaacgca agaatgacga
     7981 agaggcgttg aaggccaagt acgccttgct gaaacagctg atgggttata ccccagccga
     8041 ctcgctgcat ctgcaatatg atagtgcgca gatggaggct tctatctacc tggacacgct
     8101 ggcgcctgtg gattattcga aacgcatcga ataccagcag ctgatgaccg ggcggcggtt
     8161 gcagcaggcc gatgtcaagt ataacaaatg ggcctatctt ccttccgtca cggcgaatgc
     8221 aggctacaac atgaattatt tcaatcacag tctggataat ctctatggca acaactttcc
     8281 aagttcgtat gcggggttac aggtaaacgt acccatcttc cagggcacca aacggacaca
     8341 gaacatccgt atcgcggaat tgcagctcac ccgtatcaat tgggacgtcg tgaatctgca
     8401 gacagcgatc tcgaccgagt atgcgcaggc aatggcgagc tataaaggat atctcaacaa
     8461 ttattttgta gagcgtgaaa atttgggtct ggcgcaggag gtatacaaca ccataaaatt
     8521 gcaatacaac tcggggatca aggcctatct cgatctgatc acggccgaga cggacctgcg
     8581 gacggcggaa ctggtgtata gcaacgcgct cttccaggtc ttgagcagca agctggatgt
     8641 ggagaaggcg ttgggaacgg tacaatattg agaatgcccg gcaacggagc acatcgaata
     8701 cacaaatatt ttcttttcat atgagaagtc gtttacaagg aatgggtctt ttgtcgatgg
     8761 cgacagcgct ttgtctggcc tcctgcggga ataaaccgac gggtccccag gcaccgccgc
     8821 caaccgcggt gaatgtctat acggtagagg cgggcaacgc cacttatttc gataattatc
     8881 ctgctaccgc tacgcccctg aaccaggtgg acatcaagcc ccaggtctcc ggcaatatcg
     8941 tcgggatatt cttcaaggat ggccagcggg tgaggaaggg tcagaaattg tacgagatcg
     9001 atcagctaca atataaggcc gccgtcgaac aggcgaaagc gaacctggat gtcgccaaag
     9061 ccaatctggc gcgttctcag caggactacg accggtacca ggaactggct aaacaggacg
     9121 ccatcgcacg acaggtgctc gagcaccagg tcgccgatct ggaggccaac aaacgctcgg
     9181 tacaggcggc gcaggccaac gtcgcaagcg tggagaccaa tctgaaatat tctgagatat
     9241 atgcgccctt cgacggcacg atcggtatct cgctggtaaa ggtgggcacc tcggtatacc
     9301 cccagacgtt gttgaattcc gtctccacgg acgatccgat ggcggtggat atctcgatcg
     9361 atcaggcgga gatacccagg tttaccaccc ttttagaaaa aggcaggaat aaggattcga
     9421 cgttcacggc ggtgttaccg gatggatcga tctatcctca tcccggtgat ctgtacctca
     9481 tcgaccgctc cgtagacccg acgacgggta cgatcaaggt gcggttgaca ttccccaatc
     9541 cgaaagatga gttgaaggcg ggtctgacca cgaatatccg ggtgaaacac agttccggcg
     9601 acagcacgtt gctgatcccg ttcaagggcg tggtcgaaca gctgggagaa tatttcgttt
     9661 tcgtggctga tagcggtcac gcctatcagc agaaagtgac gcttggaccc cggatcggcg
     9721 gaatggtcgt cgcgcgcagc gggttgaagg ccggggagaa gatcgtggtg gacggcgtac
     9781 agaagctccg ggacagctcg gctattaagg cggcgccggt gaaaggaata gtcaagtaag
     9841 gaaaggaaca aataattata catgaagagt tcgaatgtat ttctgaggag gccggtcacg
     9901 gcaatcgtca tctcgctggt gatcctgctt gtgggcggtc tggccattct gaacatgcct
     9961 gtcagtcagt acccggacat tacgcctccg accgtgcaga tcaccgccaa ctttgtcggt
    10021 gccgatgcgc agacggtcga acagacgacc accacatcca tcgagacgca ggtcaacggt
    10081 gtcccgggta tcgcctatat gcaaagcaac agcgccaaca gcggtaccgc atcgataacg
    10141 gccacgctga atatcggcgc caatccggac attgtcgcgc tggacatcca gaacagggta
    10201 gggatcgcat tgcctacatt gccgacgcag gtacaacggc tgggtgtcac gacgctgaaa
    10261 cgcaaccctt cgatcctgat gctggtggct atcttttcgc caaagggcac gcatagcgtt
    10321 cccttcatgg acaactatgc gaacatctat cttcgcgata tcctgttgcg tgtaaagggc
    10381 gtaggtgacg tgatcagccg cgccgacaat ttctcgatgc gtgtctggtt aaagacggac
    10441 aagctggcgc agctgggtct cactgcgaat gacgtacaga acgccatcaa cgagcagaac
    10501 ctgcagatag ccgcgggatc ggtggcggct ccgccgcaaa actccaatgc ggccttcgaa
    10561 ttctccgtgt ttaccaatag ccggctgaat accgaagagc aattcgggaa catcgtcatc
    10621 cgcagcaatc cccagcagaa ctcgatcgtt tatctgaagg atatcgcgcg tatccagctg
    10681 ggcaaagtga gctatgccgc caactcgttc gtggacggga aaagggcctc ttacctgctg
    10741 atctaccagg ctccggggag caatgcactg gaaacggcag acggcgtgta caaagccatg
    10801 gcggatgcga aaaaatattt ccctgccgat gtcgcctata acgtcccttt cgaatcggtg
    10861 tcggtagtga aagtctctat cgaagatgtg gtccggaccc tgttcatcgc attggccctc
    10921 gtgaccttcg tggtctttat cttccttcag aactggcgcg cgaccctgat ccccgtactg
    10981 gctatcccgg tctctatcgt cgggaccttt atcttttttg ttccgttggg ttttaccatc
    11041 aatacgctga cgatgtttgg tttcgtgctg gctatcggta tcgtggtgga tgacgccatc
    11101 gtcgtggtag aggccgtgca acattatatc gatgtcgaac atttatcacc gaaggaggcg
    11161 acggcgaggg ccatcgcgga tatttcggga cccgtcatag ccattgcgct gatcctggcc
    11221 gcggtattcg taccggtagg ctttataccc ggcatcgtag gccgcctgta tcagcaattt
    11281 gccatcacca tcgccatctc ggtgctcatc tctgcattcg tggccttgtc tttgactccg
    11341 gcattgtgta tgctgctgct tcggccgcac aaggtcgata agaaagccag gggtctcagc
    11401 tgggtatttt ataaattcaa tcagggtttc catcgattta cgatgcacta tacgatcggg
    11461 gtgaagggct ggatcaaata cgcaccgctg gtactggtcc tgctggcctg tatcatcgcg
    11521 ggagatatct tccttttcaa gatcaagcct acgggattta tccccctgga ggatgagggg
    11581 cgggtgtata tctcctttga aattcccgag gcagggtcta cgacacgggc gctgaccgtg
    11641 ctggacagtg tccagcatat cgcgcagggt acacccgggg tgctccatac ggccgcgttg
    11701 ggaggcctga acatcctgac cttcggattc aaatcgaatg cgggaagtat tttcatgcag
    11761 ctgaaaccct gggaccagcg gaaatccaag gcagagcagg agaagggtgt cattgccgag
    11821 ctgaacaaac ggtttgccgc catcaaggac gcccggatca tcgtcatcga gcctccggcc
    11881 atcccgggac ttggtaatgc cggcggtttt agtatccaga tcgagcagcg gcaatcgaat
    11941 gacgatatca agaccttcga gcgggtggtc aatcagttcg tcgcagaggc gaacaaacag
    12001 ccggagatcg gacaggcctt tacctttttc ggtgcgcaca cgccgggtta tgaagtggat
    12061 gtcgacaggg acaaggccaa gaagctcggg ttgaaccttg ccgacgttta taatacgatc
    12121 cagatgtaca tgggcagcac ctatatcaat cagctgaccc tttatggccg gaattactac
    12181 gtcgttgcac aggccgatac cgggtatcgc accgagatcc acgcaatcga ccattattat
    12241 gtcaagaaca tgaatggaca gatgatcccg ctgagttcga tagtatcgta taaactgacg
    12301 gaaactgcgc cgctgatctc gcatttcaat atttaccgca gcgcagagat cgatggagga
    12361 gccaagccgg gatttagcag tggtcaggcg atacaggcgt tggaacgggt ggcggcgaag
    12421 gtgctgccgg aagggtatgg atatgactgg tcgggtctaa gtctgcagga gataaaatca
    12481 gggaacagct cggtttatat tttcatgttg tctgtcgtat tcgtgttcct gttcctggcg
    12541 gcattgtatg agagctggtc ggtgccgttc tcggtgttgc ttgccgtgcc gatcggggta
    12601 ttcggggcca tctttacttt gtggctgatc cccagattga ccaataacgt ctatgcacag
    12661 atcggactca tcaccctgat cggattgtcg gcgaagaatg cgatcctgat cgtggaattc
    12721 gccaaggagc gtgtggaccg cgggatggcc gtcgtttctg cgaccctgga tgccgtcaaa
    12781 ctccggttac gtccgatcct gatgacctcg gtggctttcc tgctgggggt attgccgtta
    12841 gctctggctt cgggagcgag ctcggttgca aggaatacga tcgggtttac cgtactgggc
    12901 gggatgtttg ccgcgaccag tctggctatt ttcgtcgtgc cggtgctgtt cgtggtgatc
    12961 acgcggttga cgtatgggaa gcggaagctg gatgaattgc gtcaccagca attgaatctc
    13021 gcggaataag ctattacggc tccccgcccc gatcggccag ggcgggtctt ttattccgta
    13081 tgtaaactgt cgactgggtt ggcggaagcc gccttcagcg cgcggaagcc gatggtcagc
    13141 agggtaatga gggtaacaag caccccggtt atcgcgaaga tccaccaggg gatcgtgatg
    13201 cggtaggcga aatcctgcag ccatttgttc atgaccagcc aggctaccgg ggccgcgatc
    13261 aggaatgcca gcgccaccag ccgcaggaaa tccttagaca gcagtgttac gatcgaactt
    13321 gttccggcgc cgagcacctt gcggatccct atctcgcgcg tcctttctgc cgcagtaaag
    13381 gtcgccagac cgaatagccc cagacaagcg acgaaaatgg tcagaccggc gaagatgccg
    13441 aggatgcggc cgatgttttg ttcggactta taggtattgt tgaaccggtc atcgagaaaa
    13501 ctgtaggaaa agggcatttc cgccttcgcg ttcttccatt cattttgcat ggttgccagc
    13561 agaccaggga ggtcttttgt cctcgctttt acgataagat tggactcatc gcctccgagg
    13621 gtcattacca gcggtgtgat cagctgatgc agcgatcgga aattaaagtc tttgactacg
    13681 ccgatgacat ggtaggtacg gctgacaccg tcgccctcgc tatgcgtgac ggtatggcct
    13741 accgcattct caccccagcc gtaagctgta gcggccgcct ggttcaggat catcgcggag
    13801 gaatcggcgc caaagctgcg ggaaaaatta cgccccgttg ccatctccat gtcgagagtg
    13861 gggatatagt cgtaatccac atcgaaccgg accgatttca ccaattgggt ggactgaccg
    13921 tcgccatagg ccataaaatt gttgctataa ctgggtcccg ccggcaggta accggaggtg
    13981 gtgatgctgg cgatccgtgg atcttgcagc aaacgttggc ggtaagcctc gaaattgtta
    14041 cccagccagt agggttcctg gaggacgatc acctgatcct tatcataacc cagtttttta
    14101 tgatggatat aggacagttg gtcataaacg atggccgtcc cgacgatcag gctgatcgag
    14161 atgaagaact ggaagacgac cagaccgcta cggagggtca tgctttttcc gccagagcta
    14221 agacggcctt tgagtacggc gatcgggcga aaggcggaaa ggaagaaggc aggatagctg
    14281 cctgacagca gaccggtgaa aaggaccagg gcgatcagca gtatcgtcag ccccggattg
    14341 gaatcccaat ggaaggacag attccggcct gcgatgtcat tgaaggcggg cagggccaga
    14401 tagacgagac cgatcgccag caggaaggcg ctggccgtca ggaggagcga ttcacccagg
    14461 aagcgacgga tcagctggcc gcgtaacgag cccagcactt tccggacgcc tacttccctg
    14521 gctctcctgg tcgcggcggc ggtagaaaga ttcatgaaat tgatacatgc gatcagcagc
    14581 ataaatatgg ctaccgcgct aaaaatatag acatagtcga tattcccgtt gggctccaac
    14641 tcgcccgtca ggtccgaatg gagatggata tccgtcagcg gctggaggaa cagaccgatg
    14701 gaattgcctt tttttctgaa ttcttcgagc gagataccca gaccttgttt cagctggggc
    14761 ccgatgtatt tatcgatctc ggcgggtagt ttggcttcca gttttcgata gtcatatccc
    14821 ttcggcagga ccaggtacgt aaagaaattg gagctcatcc aggaggagat acccgcctcg
    14881 ggcagggcgg tcatcgaggc aaaaagaccg aaatggaaat gcgaattggc gggtacgtct
    14941 tcgatctccc cggtaacgat catgggtttc agaccgccat cttttgagat cagggtcttt
    15001 ccgatcgggt cttcttttcc gaaaagccgc tcggcgactg tgcgggtgat cacgatggta
    15061 ttcgggcggt cgagtgctgt tttggcatcg ccgcggatga ggggaagggt gaagacctgg
    15121 aaaaaattgg ggtccacgaa tgcgaatttg tcctcgcgga atattttgga accgacgacg
    15181 agacggggga tgccataggt cctgatcctc gtggcggcca gcacctccgg aaagtcgctt
    15241 ttcaatgcct gggccacagg gggcatgacg ttggcctctt ttatttcacc tccctccatg
    15301 actcccctga agaatacgcg gacgatccgg tctgcctttt cattatatct gtcatagctg
    15361 agttcgtctg cgacataaag caggatgacc aggcaggcag cgattccgag tgcgagaccg
    15421 gcgatattga tgaccgtaaa ccatttgtta cgctgaaaat gacgtagggt gaagcgaaaa
    15481 aaattcccaa gcatgaagat agattttaga taggacgaca tgatcgtcgg tgaggttgca
    15541 gccaatggga tgccggaaaa gatggtgggt gatcctcagg aggttagagg gtggggtggt
    15601 agagggggtg ttcagttccg atacaggtag tgttcggttt gtatcgtata ttagagggga
    15661 cggaggaccg ctaatttctt tggttttatg gagagaggcg gattttggag gttcagagaa
    15721 ggcaccaatt tatttagcat tttagcaatt ggcttaagaa actgtttttc aaaatattga
    15781 attgatttat ttaagatttt aaataggatg aatgactagc tgatttggat gaattcgtgg
    15841 ttttttgaga attgatgata ttgcccgaaa atttgtaaaa taaaatgaac ttaataattg
    15901 acatacaata ttttgcgcct tttagctact ataaaatttc gtataaatcg tcgaatatca
    15961 tttttgagca atacgaaacc tggcaaaaat tgagttttcg caaccgttgc cggattgcgg
    16021 gggcggaagg ggtcatcgat ctgagcattc cgttgtccgg agggagagat cagaagaccg
    16081 tgatgaagga ggtgaggatc gcggatggca agggttggca ggaccggcat tggaagacga
    16141 ttgtttcctg ttatagccgg tcgccctggt tcgatcatta ccgggatgag ctggaggggc
    16201 tgtaccggcg gccggcgaat ttcctgctgg actggaacct gctttgtttc gagtggtcga
    16261 tgaaggcgtt ggggatgagc cggccggtgc aattgagcca ggaatatcgg aagacttata
    16321 gccctgagga ggggttagac tggcggggca ggatcgtgcc gggagcggcg ggggaggtgc
    16381 cgggagctgg taaggctccc gtgccgggag ctgccgggga ggtgccgaag tatcggcagg
    16441 tattcgaaga gcgcatagga ttcatacccg atctttcgat cctggatgtc cttttttgcg
    16501 aggggaggga agccattggt tatgtgctgg cgtgaggggc gatattgcga atgaggcaag
    16561 ccattggcaa tgcgctggcg tgaggggcga tgctgcgagg gcgggaagcc atcagatata
    16621 tacggtcgtg agacggcggg cggcctgttt cagctgacgc tggagatcgt ccagtcggag
    16681 catgtatcgc tgctgcaaaa ggagctggtc tttgagcagc tgtttcagct gtttgcggga
    16741 ggcggattgc agatcagtcg acaacgtcgg gttcctggaa gccttgtcaa gcgccgcctg
    16801 cgccgcctgg agtgcgttca ggcgggtctt tagttcttcc cggagctggc ttatctgaag
    16861 ccggatcgct ttctcggtga ccttttccat tatcgccaga cgatcttccg ggggcagcgt
    16921 atcggtataa tgaaaagaga agctggattg gggaacgaag ggaaatcctt cgctggctgt
    16981 gggtggggca acggccgtct ggccattgcc ggacggagag ccgatagagt aatcccggct
    17041 gtcgataacg accagatcgg cataggcggg tgcgggagta ttcgaaggtc cggaggttcg
    17101 gaaccaggta gcttcgtctt cattgaaggc tccggcggcc ggcggatgcg tcgctcggcg
    17161 gtggctgaca ggcgaagccg cgccatggtg ggtagctgag ggagccgaag catgggcgat
    17221 ggcgcgagtg gctatatgcg ttggcgggag atgcgcgata gaaagattcg ttggcgggag
    17281 cgatttaacg ttcgaagatg cgatattcac gattagtcgc ttgtcggtct caatgtaggg
    17341 atgcgcagtg ccggccggcc gcatcatggc gaccgaagca ggtggaagcg gtggtgtcat
    17401 tctccggggt ccccaaaaaa gaagggtcat tcctatcgtc aggaatagca ggaaaagcgg
    17461 gcgcgcgccg gggcggtcgt ccgacctgtg gtgctgcagg atctttttgg ccctttggag
    17521 aagcaatttg ttatcgcctc cggtggccgc taccgccaac cggccatgcc ggtgctgtcg
    17581 cgccagggat aagagggcgg aaacataggc atgggcgtcg tattggaatt ggagtacgag
    17641 gtcgtcgcag cagtgctctc tttccttttt cagctgaccg atcagcatgc gggcaaacgg
    17701 gttgaagaaa aacaccagct ccatcaccgt taccaaaata ttgatgagat agtcttttct
    17761 tcggatatgc gccagttcgt ggacaaggat cgcttcgatc tgttgcgtcg taagatgggt
    17821 gatcatcgct acgggaagca ggatcatggg gcgcagaaaa ccaagggtca cgggtacatc
    17881 caccagcgaa gacaaatgga cttttacgac gggtaagata cccatgttgc gtgccgtttc
    17941 ggcgacgaat atgcggaaac cgggccctat tttcgagaga cccattttgg tgatccggcg
    18001 ggaacgcaga taatgacccg tataatgaat aagtaacccg cccaatacga ggagataccc
    18061 gaaggagcac caggataatc cttcatcgat gaaccagcgg gagacggaca ggagccggtt
    18121 cccgtagggt tgcagcgagg ccggcagcag gtaagcgccg gtagcgggtc ccagccagta
    18181 tgggctgtcc gccgtgccca gccaataggt cgtaatgaag gtcaggatag tccaggccgc
    18241 tccgatggtc aggagcattg cggcgagacc gtgccgcatg cgggcgctgg catcgcggaa
    18301 gatcaggacg aacaggtgat agatcgccca tagcaaactc atttgccaca ggctgttgaa
    18361 tagcgaccag cccagcgctt tcagtaaagc ggattgggtc tacacttgct tttattgctc
    18421 tttcagatta ttcaataaca gctggatctc ttcgagctcc gcgggggaag ctttatggtt
    18481 gccgagtgcc tgcaggacca gctcgcccgg agagcctccg aacaaggtat tgatcatttt
    18541 acccagcagg tgtttctgcg ttttttcctt actgacggag gcctgataga tatgcgtttt
    18601 gaaagaatcg tcccttttta ccaggccctt gtcgaacatg atctgcatca gcttgagcgt
    18661 ggtagtgtag cccacgtctt tgcatttaag cagctcttca tgcacatccc ttacgctcgc
    18721 ctgatccctt tcccagagga cctggaggat ctcgagctca ctttcggtgg gtttgatcgt
    18781 cttcgtcggc atatgacata gagatttaga cttcaattta cgatattttt cgtaaatgat
    18841 tgttaaaggg ggcaaatttg ggcggccggc gaaaatggtg aattcggcgg cggacggaga
    18901 tggtaagggc ggagggattc gcgcgggcag ccgggacaaa aaaaagcccc gatgtggaaa
    18961 caccgggact ttggtatttg gatctgatta gtgtcgttgt tcgagttgta aagatatatt
    19021 taaaaacaaa atagccaaat ttttttttca acgataggcc gcgactgtgc cgggggccgg
    19081 agccttcacc tgtgcggcgg ccaccttttt ctccacggga tgtttttggc cggcaagacc
    19141 ggtgtcgcgg gtatggaaga tagacgccag ggtaggcgct atcaccaggg agactatcga
    19201 catcagcttg atcaggatgt tcatggaggg tccagaggta tccttgaagg gatcgccaac
    19261 ggtatcgccg gtgacggatg ctttgtgcgg atcggatttc ttataatgga tctcgccatt
    19321 gatctccacg cctttctcaa acgatttttt ggcattgtcc cacgcgccgc cggcattgtt
    19381 ctgaaagatg cccatcagca cgccacagac ggtagcgccc gccaggaagc cacccagtgc
    19441 ttcgggtccg agaatgaaac cgatgatcag cgggaaaagg atggcgatag cgcccgggag
    19501 caccatcttc ctgatggagg cctgggtgga gatggcgacg catttgtcgt attcaggctt
    19561 gcctgtgccc tccatgatcc cgggtatcgt ccggaactgg cggcgtacct cctcgaccat
    19621 tgccatggcg gcctgaccga ctgcctggat tgcaagcgag gaaaagatga acgggatcat
    19681 gccgccaacg aaaaggctgg cgaggacctt ggctttgtaa atatcgatac cctggatacc
    19741 ggcaacgccg acaaaagcgg cgaaaagggc cagcgcggtc agggcagccg aagcaatggc
    19801 gaaaccttta cccgttgcgg cggtggtgtt tccgactgca tccagtacgt ccgttttttc
    19861 ccgcacgtcc ttgggcagct cgctcatttc ggcgatgccg ccggcgttgt cggcgatggg
    19921 accgaatgcg tcgatcgcta gttgcattgc cgtggttgcc atcataccgg ccgcggcgat
    19981 cgccacacca tagagaccgg cacaggcaaa ggagccccag attccgccag caagtacgag
    20041 aatgggcagg aaggtggatt gcatacccac ggccagaccg ccgataacgt tcgtagcgtg
    20101 accggtacca gactgacgga tgatgctaag gacaggcgcc ttacccattg cggtatagta
    20161 ttcggtaatg atgctcatca gcgtgccgac aagcagaccg acgccaattg ctccgagcac
    20221 accccatttg gtaaactccg taccgcggag ggtcatcgtg tcgggcatta tataataaac
    20281 gaggaagata caggcgatgg cggtcattac gatagaaccc cagttgccca tattcagcgc
    20341 tttctgaacg gtggaagtgt taacagccga cgaatcgctg atctttacga aaagcgtgcc
    20401 gacgatgctg aagataatac cgatgcccgc gatgagcatg ggaaggagga tgggagacaa
    20461 acctccgaaa ttgtcatcgg agatggtttc ctgtcccagc accatcgtcg ccagtacggt
    20521 ggcaacatag ctgccgaaca gatcggcgcc catgccggcc acgtctccaa cgttatcacc
    20581 gacgttatcg gcgatagtgg cagggttgcg gggatcgtct tccgggatac cggcttcgac
    20641 cttaccgacg aggtccgcgc cgacatcggc tgccttggta tagataccgc cgcctacacg
    20701 ggcgaagagt gctatggatt ccgcgccgag agaaaagcct gtcagcactt cgatcgtgag
    20761 tctcatttcg tctgagttga ccggggcacc cgaagcgaaa atggctctca ggaggatgaa
    20821 cagaccgccg agaccgatga cagccagacc ggcgacaccg agacccatta ccgagccgcc
    20881 cgtgaaagat accttcaggg cctgtgcgag gctggtccgg gccgcatgtg ccgtgcggac
    20941 gttcgatttg gttgcgctgc gcatgccgat atagccggcg gttgcgctca atacggcccc
    21001 caggatgaag gagatggcga tggaccagtg agaatgaggg ttcgtgtaac ccatgaaggc
    21061 cagcaggatc gcggccagga tcacaaaata ggagaggatc ttccattccg ccttcaggaa
    21121 agccatagcg ccctgggcga tatgctgacc gatctcctgc atgcgcgcgt tgccggggtc
    21181 ctgttttgaa acccatgaga acttgataaa ggtgtagacg aggccgacgg ctccgagaac
    21241 aggaaccaga taaagcaaat tgtccataag tatataatcc ttaaagcaag ggacggcaaa
    21301 aataggggaa aaaaagaaat aaggccgtgt cttttgacac ggccttcgaa gttagggggt
    21361 tcgggttcag gggtctgcct gggggcgggg tttagggt
//
LOCUS       DASYSV010000498         4620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_226793, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000498 DASYSV010000000
VERSION     DASYSV010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226793"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VGQ51_15385"
     CDS             complement(<1..1444)
                     /locus_tag="VGQ51_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEV9038017.1"
                     /translation="MTGSTIPSSKTQIFQTNSPGRWQRLKWGGRILLLFLVIGGVVFS
                     IAISRDYKPALPRLKEQSQLYKKVLDTNRTFLFKNSLIEQYGGFRKYINEKVPYRGGA
                     FPGQSKKDSERAALIRAHRSDTSFRSFTKFPAGIRAAFYVDWDAQSFLSLQQYIDKIN
                     MVIPEWIFIDPTADTVFTDIDPRAWGVMKHSGVKILPILSNNYKGDFRGDGVHRIIND
                     PVKEQRLINDVIRILEKNNFVGVNVDFEELQEKTNEKLVDFQKNLYEQMHERHLLVTQ
                     DVIPFNEDYDFAGLAKYNDYLFLMAYDQFSDNSGPGPIAHQKWIEGAVDDAVKNIPEQ
                     KLILCLAAYGYDWRLGKEMSSRTVSYQQALFTAKDNDANIDFDNDSYNLHYNYDDDDG
                     VRHQVQFCDAATIFNALRFATEYGIAGTSIWRLGDEDPRVWEFYDQDMRKSALSHFDF
                     SAFSKVDAKAGSDFVDYEGSGEVLDIIATPT"
     gene            complement(1524..3323)
                     /gene="dnaX"
                     /locus_tag="VGQ51_15390"
     CDS             complement(1524..3323)
                     /gene="dnaX"
                     /locus_tag="VGQ51_15390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV9038018.1"
                     /translation="MGVLPMDKFIVSARKYRPQDFSTVVGQSHITTTLKNAIRNRQLA
                     HAFLFCGPRGVGKTTCARILAKTINCENVSPEGEACNKCTSCVSFNEGTSLNIHELDA
                     ASNNSVDDIRTLVEQVRFAPQAGKYKVYIIDEVHMLSTSAFNAFLKTLEEPPPYAIFI
                     LATTEKHKILPTILSRCQIFDFKRITSTDTVAHLEDICKKEEIRAEKPALQIIAQKSE
                     GCMRDALSILDKIVSFTNGELTYANTLEHLNILDADYYFRILEAMQQQNLSEALLTYD
                     SINRKGFEGDLVLNGLAEFIRNLLVCKDEKVASLLEVVESFRDRFVAAAGKSDTLYLI
                     NALNVLNDAEINYKSARNKRLHVELALIKLCYLAQALQVSSGESGSKKKQPESLRPVS
                     FRNIPPIEPVRKTAKQRDGEETGGGGLPEAGKNPGSGGAKLIVEEGVAAEKVTTGLAG
                     SSGSLSRIRQQMASRRQNDEAGCSRPLDAETLHKAWKQYADFLRENRNPAVQSLELAV
                     LRIMDDQNFEVITSNNLEQKFIEQEKRALSEHLQKTFANKTISFTVVIEEKTEAHEEP
                     AGPLNKREQFKQIVEQYPLVKELKDRLRLELDY"
     gene            complement(3709..>4620)
                     /locus_tag="VGQ51_15395"
     CDS             complement(3709..>4620)
                     /locus_tag="VGQ51_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038019.1"
                     /translation="PVGGIGFFDCINDQAAANMLFDVAKHWLLQRGMQAMDGPINFGE
                     RDRWWGLLVEGFHEPLYGMNYNPPYYKDLFENYGFKPFFHQLCFGMEPMRPLNDKVLQ
                     RHAAVAGDPGYSARRIDKRQLEKYADDFLVIYNGAFAGHGGMKQMRKEQAIQLFKKMK
                     PLLDEDIVWFAYYQEKPIAMFLNIPELNQYFKHFDGKFGLLQKLQFLWLRSRGACRHF
                     TGVVFAVVPAFQGMGVDAYIIVELAKVVQPANFYTHYEMQWIGDFNPKMVNVAKGLGD
                     AVVSRKLTTYRYLFDRSREFKRHPMLA"
BASE COUNT         1042 a         1290 c         1073 g         1215 t
ORIGIN      
        1 tggtgggtgt agcgatgatg tccagcactt ctcccgatcc ttcataatcg acgaagtccg
       61 agcctgcttt tgcatctacc ttactaaatg cggaaaaatc gaaatgcgac aaagccgatt
      121 ttctcatgtc ctggtcatag aattcccata ccctcgggtc ctcgtcgcca agccgccaga
      181 tcgaggtgcc cgctatcccg tattctgtgg cgaaacgcag cgcgttaaaa atggtcgcgg
      241 catcgcaaaa ctgcacctgg tgccggacgc cgtcatcatc atcgtaattg taatgcaggt
      301 tgtaggagtc gttgtcgaaa tcgatgttgg catcattgtc cttggcggtg aacaacgcct
      361 gctgatagct gaccgtccgc gacgacattt cctttcccag ccgccagtca tagccgtaag
      421 ccgccagaca aaggatcagc ttctgctcgg ggatattctt cacggcatcg tccaccgctc
      481 cctcgatcca tttctgatgc gcgataggcc ccggacccga gttgtccgaa aactgatcat
      541 atgccatcag gaacaaatag tcgttgtatt tcgccagacc cgcaaaatca taatcctcgt
      601 tgaaggggat cacgtcctgg gtcaccagca gatgccgctc atgcatctgc tcgtacaggt
      661 tcttctggaa atccaccagc ttctcgttgg ttttttcctg cagctcctcg aagtccacgt
      721 ttactccgac gaaattattc ttttccagta tcctgatcac gtcattgatc aatcgctgct
      781 ccttgaccgg gtcgttaatg atcctgtgaa ctccatcccc gcggaaatcc cctttatagt
      841 tgttggaaag gatcggcagg atcttgaccc cggaatgctt cataactccc caggcccgcg
      901 ggtcgatatc cgtgaacacc gtatccgcgg tggggtcgat aaagatccat tccggtatga
      961 ccatgttgat cttgtcgata tattgctgca acgacaggaa ggactgggcg tcccagtcta
     1021 catagaacgc cgcccggatg ccggcaggaa atttcgtaaa agaacggaag ctcgtatcgc
     1081 tgcgatgcgc acggatcagc gcagcccgct cggaatcttt tttggattgc cccgggaatg
     1141 cccctccccg atagggaact ttctcattga tgtatttccg gaaccccccg tactgttcta
     1201 taagactatt cttgaaaagg aaagtgcggt tggtgtcgag tacttttttg tacagctggc
     1261 tttgctcctt caatcttgga agcgccggtt tataatcccg cgaaatagcg atagagaaga
     1321 cgacgccgcc gatgaccaaa aaaagtaaca ggatccttcc gccccatttg agccgttgcc
     1381 accggcccgg tgaattcgtc tggaagatct gtgtttttga agaaggtatc gtgctgcctg
     1441 tcatagatta ggtacaaatt ggcaaaattc ccgccaatca gggaaatgtc aaaaatctgg
     1501 agatcctttt aacaacaaat tacctagtaa tccaactcca accttaaccg atctttcaac
     1561 tcttttacca gcgggtattg ctctacgatc tgtttgaatt gctcccgttt gttgagggga
     1621 cccgccggct cttcgtgtgc ttcggtcttt tcttcgatca ccactgtaaa ggaaatcgtc
     1681 ttatttgcaa acgtcttttg cagatgttcg gacagggctc gtttttcctg ttcgatgaat
     1741 ttctgctcca ggttgttgct cgttatgacc tcgaagtttt gatcatccat gatcctgagg
     1801 accgccagct ccagcgattg taccgccggg ttcctgttct cacggagaaa gtccgcatat
     1861 tgcttccagg ctttgtgcag ggtctccgca tccaacggcc ggctgcagcc ggcctcgtcg
     1921 ttttgccgtc ttgaagccat ttgctgcctt atcctcgata aggatcccga tgaccctgca
     1981 agccctgtcg taactttttc ggcggccaca ccctcttcaa ctatcagctt cgctccgcca
     2041 ctgcccggat ttttccccgc ctccggtaat ccgccgccac ccgtttcttc tccgtcccgc
     2101 tgtttcgccg tcttcctcac cggctcgata ggcgggatat tgcggaagga cacaggcctc
     2161 agcgactcag gctgtttttt tttagaaccc gattccccgc tgcttacctg caaggcctgg
     2221 gccaggtaac acagcttgat caacgcaagc tctacatgca accgcttgtt gcgcgcggac
     2281 ttataattga tctcggcatc gttcaataca ttcagcgcat tgatgagata cagcgtatcg
     2341 cttttccccg ccgccgcgac aaaccggtcc ctgaaactct ccactacttc cagcagtgaa
     2401 gcgaccttct cgtctttgca gaccagcagg ttccggatga attcggccag cccgttgagc
     2461 accagatcgc cttcgaaccc ctttcggttg atggagtcat aggttaacag cgcctccgaa
     2521 aggttctgct gctgcatagc ttcgagtatg cggaaataat agtccgcatc caggatattc
     2581 aaatgttcca gcgtattggc ataggtaagc tctccgttgg taaagctgac gatcttgtcc
     2641 aggatgctca acgcatcccg catgcaacct tcgcttttct gggcaatgat ctgtaatgcc
     2701 ggtttctccg ccctgatctc ctcttttttg cagatatctt ccagatgcgc caccgtatcg
     2761 gtagacgtga tgcgtttgaa atcgaagatc tggcaacgcg acaggatcgt aggtaagatc
     2821 ttatgctttt cggtcgttgc caggataaag atggcataag gcggcggctc ctccagcgtc
     2881 ttgaggaatg cattgaaggc cgacgtgctc aacatatgca cctcatctat aatgtatacc
     2941 ttatattttc ccgcctgtgg cgcgaaacgc acctgctcga ccagtgtccg gatatcgtcc
     3001 accgagttat tgctcgctgc atccagctcg tgtatattca gcgaggtccc ctcattaaaa
     3061 gatacacaag atgtacactt gttgcaggcc tcgccctccg gggacacgtt ctcgcaattg
     3121 atggtcttgg cgaggatgcg cgcgcaggtg gtcttcccca cgccgcgggg accgcaaaaa
     3181 aggaatgcat gggccagctg ccggttccgg atggcgtttt tcagggtagt cgtgatatgc
     3241 gattgcccca ccaccgtcga aaaatcctgg ggccggtatt ttcttgccga aacgataaat
     3301 ttgtccattg gtagtactcc catggcaaac gtacaacttc cccgccattt ttccgatgct
     3361 gcactgccag gtacttcgat ccgccgggcg aaaaacaaaa gaccggctct atacgagccg
     3421 gtccctgcaa ttttgtccag ttttgaagag gcttaccttc attggggata ttggatctta
     3481 acattttttc cctgacatgg tttccgaagg ttggacaacg gtttttaaag gaaatggatt
     3541 cgttggctta taggtccttt ctaaagattt tttcctgtcc gtgtgttcta atgaattcac
     3601 aattggtgcc agtttgtcac tctctggaga tcaatgtctt gtaaaaaacc catatatgaa
     3661 aaaactccct tttccggagt ggattccgaa aaagggaata tatcccatct acgccaacat
     3721 cggatgccgc ttgaactccc ggctcctgtc gaataaatac cggtaggtcg tcaacttccg
     3781 gctgacaacc gcgtctccca gcccctttgc cacattcacc atcttgggat tgaaatcccc
     3841 gatccattgc atctcataat gcgtataaaa gttggctggc tgtacgacct ttgccagctc
     3901 tacaataata taagcatcca cgcccattcc ctggaatgcc ggaacgacgg caaacacgac
     3961 cccggtaaaa tggcggcagg caccccggct tcgtagccaa aggaattgca gcttctgcaa
     4021 caagccgaac ttcccgtcga agtgtttgaa atactggttc agttccggaa tattgaggaa
     4081 catggcaatg ggtttctcct ggtagtaggc gaaccagacg atatcctcat ccagcaacgg
     4141 cttcattttt ttgaatagct gtatggcctg ctctttccgc atctgcttca tccctccatg
     4201 ccccgcaaag gccccattat agatcaccag gaaatcatcg gcatacttct ccagctgtcg
     4261 cttatcgatg cgccttgcgc tatacccggg gtcgccagcc actgccgcat gacgctgcaa
     4321 taccttgtca ttcaatggtc tcataggctc cattccgaaa cacagctggt gaaagaaagg
     4381 tttgaagcca tagttctcga agaggtcttt ataataaggt gggttatagt tcattccata
     4441 aagaggttcg tgaaagcctt ctaccagcag tccccaccac cggtcccgtt cgccaaaatt
     4501 gatgggcccg tccatggcct gcataccgcg ctgcaataac caatgttttg ccacatcaaa
     4561 caacatattg gcagcagcct ggtcattgat gcaatcaaaa aatccaatgc ctcctacggg
//
LOCUS       DASYSV010000499         6972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_358349, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000499 DASYSV010000000
VERSION     DASYSV010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358349"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /gene="mltG"
                     /locus_tag="VGQ51_15400"
     CDS             <1..906
                     /gene="mltG"
                     /locus_tag="VGQ51_15400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV9038020.1"
                     /translation="YPGVFNLLEKDTVLRSPTVFNWMAKRLDYAGNVRAGRYEIKKDM
                     SMLSIIRMLHNGRQSPVKIVITKFRTPEGFAGAVGKKLECDSAAIAAFIHNNDSLREF
                     GVDSSTFLTIVMPNTYTYFWNIPPSAIFRKMYAFYKGWWTPARTAAAQARGLTPASAT
                     ILASIVEEETNIPSDKGKIASVYLNRLSKGMRLDADPTVKYAMRDFDLKEIFYVYLKT
                     ESPYNTYIHTGLPPGPICTPSEATLQAVLASPSTDYLYFAAKPDFSGYSNFAATFKEH
                     EAYAKAWTDALKKQRAIRDSITRPK"
     gene            913..1857
                     /locus_tag="VGQ51_15405"
     CDS             913..1857
                     /locus_tag="VGQ51_15405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV9038021.1"
                     /translation="MIKLERKLYNTPPFRFLEAKSKKIILPGFQGVSLFEIVTFFSSQ
                     VKKVGLIDRARAISFSFLIAIPAAMIFICTLVPYLPVSHQIERQLLLLTKDLTPNQNT
                     YVLVSQFLVDFLEKPRAGLLSFGFVLALFYSSNAMMGIMRSFNKSVIYNSRRNPVQTR
                     WMAIKLTALVLCMVIGSVIILVTQAELLHTFLKALHISNKDHSARVLFRTLRWVVIIP
                     LFYLAIACIYKYGPAMQKRWSLFSPGTLLATLLTILTILFFSYWVNRFSTYNKVYGSI
                     GTVMILMLLVYLNSLVLLIGYELNVTIHHLKVLAGEKR"
     gene            1884..2477
                     /locus_tag="VGQ51_15410"
     CDS             1884..2477
                     /locus_tag="VGQ51_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV9038022.1"
                     /translation="MKLLLLGLLLPALGLSLHESNLPIGSSLPKADVLLKDVSGREIS
                     LGQAKEAGGLLVMFSCNTCPYVIRNQSRTKEICTYAADHHIGVILLNANEGDREGGNS
                     FGAMQEYAKSQGYHWYYALDGKSDLAGVFGASRTPECFLFDKNGKLVYHGAIDDSPGD
                     DRQVHRQHLRLAIDETLAGKEVTVKETRSVGCSINRG"
     gene            complement(2499..2978)
                     /locus_tag="VGQ51_15415"
     CDS             complement(2499..2978)
                     /locus_tag="VGQ51_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV9038023.1"
                     /translation="MRIVLLLSLLAMVASATGQTVRKIKITDLQNTIAKSDHPLIVNF
                     WATFCEPCNKEIPYFQQTISRYADQRVELILVSLDLPDYYPARIANFARSRKYTSTLL
                     WLDETNADYFCPKLDARWTGGIPCSLFINNKTHYRRFFDRQLTEPQVEIEIKKLVAP"
     gene            complement(2990..3580)
                     /locus_tag="VGQ51_15420"
     CDS             complement(2990..3580)
                     /locus_tag="VGQ51_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV9038024.1"
                     /translation="MTPERIERITQVLDKRKDDLTIVLENVFDPHNISAVMRTCDAVG
                     VQDVYILNTRIPRHKKWGAKSSSSAAQWLTIHQYTDAGECFAALRDRADHIFTTHLSG
                     DAVSLYDMDLTGRIALVFGNEHSGVSEEIRALADGNFIIPQVGIIKSLNISVACAVSL
                     YEAFRQKHSAGHYRQRSLEPGRYEQLRKEWSRELPT"
     gene            3750..4046
                     /locus_tag="VGQ51_15425"
     CDS             3750..4046
                     /locus_tag="VGQ51_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEV9038025.1"
                     /translation="MLNSPRTVELMETDALTTHDDPFSLIVWNDEVNTFEWVIETLIE
                     VCGHSVEQAEQCAMIIHTKGKYAVKRGSYDELKPVCDAITDRGIGATIEILSGS"
     gene            4072..4719
                     /gene="aat"
                     /locus_tag="VGQ51_15430"
     CDS             4072..4719
                     /gene="aat"
                     /locus_tag="VGQ51_15430"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEV9038026.1"
                     /translation="MSLFILNHELIFPPVQLAEPDGLLAAGGDLSTERLLLAYRQGIF
                     PWYEGRHILWWCPDPRFVLFPGELKESKSMKQLLRREAFDFRTDTAFGEVISNCKTIA
                     RKGQESTWITDEVRSAYTRLHGAGYAHSAETWYEGQLVGGLYGVRMGRVFFGESMFSR
                     ASNASKYAFIRYVHQLRDEGVELIDCQVYTEHLESLGARMIPRGNFISLLDSLIK"
     gene            4816..5460
                     /locus_tag="VGQ51_15435"
     CDS             4816..5460
                     /locus_tag="VGQ51_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038027.1"
                     /translation="MKKFLLAAICLPALTILHAQDLTVTKAPSMSMHKYAPFKLDFST
                     GFAIPSGSGAKAGVSFALEPKYAIIDKFAVGLRLEAAITARGWVASDGSSASASVAAS
                     ASYLATYDYYLPGLIFRPFVGGGTGVYNMASASVAADNQANTSVGTSASTKVGTMFRT
                     GFEFHHFRMAFEYNVIGKTTQTVTDGSGNTVGTISAKNSYANIKIGFFFGGGKR"
     gene            complement(5512..6885)
                     /locus_tag="VGQ51_15440"
     CDS             complement(5512..6885)
                     /locus_tag="VGQ51_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEV9038028.1"
                     /translation="MYAIVDIETTGGYAANNAITEVAIVLHDGNRELKRYETLVRPGM
                     LIPRYVQALTGITDEMVADAPDFEIIAPVVSEWLRDAVFVAHNVNFDYSFLRHQLQAC
                     GFELNSKKLCTVRLSRKVFPEAQRYSLGNICRYLGIDISNRHRAGGDADATTLLFEKI
                     LQSGGLEIIRGMLKGRNSEQYLPIHLPAGQLEILPQVPGVYYFHDQKGKVIYVGKAKN
                     LRHRVASHFSNNKTGRQKQEFLRNIYSISHETTGTELMAFLLECIEIKRLWPAYNRSM
                     KRFEATYGLYVYEDLNGYSRLILERRKRQLRPVYTFSMLAEGRNILRKLIREFRLCPK
                     LCFIQRDNDGCEGMREETCDGACEHRESPETYNGKVRAAIATLLESLPSFTVMDRGRH
                     GEEKSCILVEQGRVYGMGYLPADATIYDASELKQYLTPCTENDYMRGLIYSYVERWPA
                     KKIMIQL"
BASE COUNT         1633 a         1886 c         1779 g         1674 t
ORIGIN      
        1 tatcccgggg ttttcaatct gctggaaaag gatacggtac taagaagccc gacagtcttc
       61 aattggatgg ctaaacgatt ggattacgcc ggcaacgtac gcgctggcag gtacgagatc
      121 aaaaaggaca tgagcatgct gagtatcatc aggatgctgc acaacggccg gcaatcgcct
      181 gtaaagatcg tgatcaccaa attccggact cccgaaggat tcgccggcgc ggtcggcaaa
      241 aagctggaat gcgattcggc cgctatcgcc gctttcattc acaataatga ctccttacgc
      301 gaattcggcg tcgattccag caccttcctg acgatagtga tgcccaacac ctatacctat
      361 ttctggaata tccctccgtc cgctattttc cggaagatgt acgcctttta caagggatgg
      421 tggaccccag ctcggaccgc ggcggcacag gccagggggc tcacaccggc ttcggcgacc
      481 attctcgctt ccatcgtcga ggaagagacg aatataccct ccgataaagg caagatcgcc
      541 agcgtctatc tcaaccgcct gtccaaaggt atgaggctgg atgccgatcc gaccgtcaaa
      601 tatgccatgc gggatttcga tctgaaggag atattctacg tctatcttaa gaccgagtca
      661 ccgtacaata catatattca taccggtctg ccgccgggtc ccatctgcac gccctcggaa
      721 gctacgctgc aagcggtgct ggcctcccct tccacggatt atctttattt tgcggccaaa
      781 ccagatttta gcggctattc caattttgcc gctaccttta aagagcacga ggcatacgcc
      841 aaagcctgga ccgatgcgct caaaaagcaa cgcgccatca gggatagtat caccaggcca
      901 aagtaacgag ctatgatcaa gctggaacga aagctataca acaccccccc tttccgcttc
      961 ctggaagcca aaagtaaaaa gatcatttta ccgggctttc agggtgtgtc gcttttcgag
     1021 atcgtcactt ttttttccag ccaggtaaaa aaggtaggtc tgatcgaccg cgcgcgggcc
     1081 atttctttca gcttcctgat agccatcccc gccgccatga tcttcatctg tacgctggtc
     1141 ccctatctgc ctgtttcgca tcagatcgaa cgccagctgc ttcttcttac gaaggatctt
     1201 actcccaacc aaaataccta tgtgctggtc agccagttcc tcgtggactt tctcgaaaaa
     1261 cccagggcag gcctgctgtc cttcggtttc gtcctggcgc tgttctattc ttccaatgcg
     1321 atgatgggca ttatgcggag ctttaacaaa tccgttattt ataacagccg gagaaatccg
     1381 gtacaaacgc gctggatggc catcaagctg accgcattgg tcctttgcat ggtcatcggc
     1441 tccgtgatca tactcgtcac ccaggccgag ctgctgcaca ccttcctcaa ggccctgcat
     1501 atatccaata aggaccacag cgcacgcgtg ctcttcagga ccctgcgctg ggtcgtaatc
     1561 attccgctgt tctacctggc cattgcctgc atctacaaat atggaccggc catgcaaaaa
     1621 cgatggtcgc tgttctcccc cggcaccctg ctggcgacat tgctgaccat tttgacgatc
     1681 ctcttcttct cctattgggt caaccggttc agtacgtaca ataaggtcta tggctctatt
     1741 ggcactgtca tgatcctcat gctgttagtg tacctgaact cgctggtttt gctcatcggg
     1801 tacgaattga acgttaccat ccaccatctg aaggttctgg caggtgaaaa acgctaactt
     1861 tgttccaaca aaaatcctgg aatatgaagc ttcttttatt gggtctttta ctgccggcac
     1921 ttggcctgag ccttcatgaa tccaacctcc cgatcggatc atcgctcccc aaagcggatg
     1981 tcctcctgaa agacgtctcc ggcagggaga tcagtctcgg gcaggcaaag gaagcgggcg
     2041 ggctgctcgt gatgttctcc tgcaacacct gtccctatgt catccgcaac cagagccgta
     2101 caaaagagat ctgcacctat gcggcagatc atcatatcgg agtcatcctg ctcaatgcca
     2161 acgaaggcga tcgtgaaggt ggcaactcgt tcggagcgat gcaggaatat gcaaaatctc
     2221 agggttatca ttggtattat gcgctggacg gcaaaagcga tctggcgggt gtgttcgggg
     2281 ccagcaggac gccggaatgt tttctgttcg ataagaatgg gaaattggtg tatcacggcg
     2341 ccatcgacga ctcgccgggc gacgatcggc aggtccatcg gcagcatttg cggttggcca
     2401 tcgatgagac ccttgccgga aaggaagtga cggtaaaaga gacccgctcg gtcggatgtt
     2461 cgatcaaccg gggttagaca caatgacggc cgtgcccctt atggcgctac cagtttcttt
     2521 atttctattt ccacctgggg ctcggtcaac tgccggtcga aaaaccggcg gtagtgcgtt
     2581 ttattattga tgaacaacga gcacggtatt ccgcccgtcc agcgcgcatc cagcttcgga
     2641 caaaaataat cggcattggt ctcgtcaagc cacagcagcg tcgaagtata ctttcgactg
     2701 cgggcaaaat tggcgatacg cgcaggataa tagtccggaa gatccagact gacaaggatc
     2761 aactccacac gctggtccgc gtagcgcgaa attgtttgtt ggaaataagg gatctctttg
     2821 ttgcagggct cgcagaaggt agcccagaaa ttcacgatca gcggatgatc gcttttggcg
     2881 atcgtgttct gcagatcggt gatctttatt ttccgtacgg tttgtccggt tgcggacgcc
     2941 accattgcca gcagtgataa caataatacg attcgcatag ataatatatt taggtcggta
     3001 attccctgct ccattctttc cgcagttgtt cataacgtcc gggttccagg ctgcgctgcc
     3061 ggtaatgtcc cgcggagtgc ttctgacgga aggcttcgta aagactaacg gcacaggcaa
     3121 ccgagatatt cagggatttg atgataccca cctgcggaat gatgaaattg ccgtctgcaa
     3181 gcgcgcggat ctcctcgctg acgcctgaat gctcgttccc gaagaccagc gcaattcttc
     3241 ccgtcagatc catatcgtaa agcgagacgg catcgccgga caaatgcgtc gtaaaaatat
     3301 gatcggccct gtcacgcagt gccgcgaaac attcgccggc atcggtatac tgatggatcg
     3361 tgagccattg cgctgcactg ctgctgcttt ttgcgcccca tttcttatgc cgcggaatac
     3421 gcgtattgag gatatagaca tcctgcacgc ctacggcatc gcaggtgcgc atgactgcag
     3481 aaatattgtg ggggtcgaaa acgttctcca gtacgatggt gagatcgtcc ttccgtttgt
     3541 cgagcacctg cgtgatcctt tctattcttt ccggggtcat atttcgaagg tccggcaatg
     3601 ccggcccgaa tacaaaaata gaccaaaatc gcataattaa atatcaatca gatagtaagg
     3661 cactccatag cctgcgccag cgcaagttat gtcttgcata ggcagccgaa ttgtgtaatt
     3721 ttgcagaaag ctataatcgc atgcccttta tgctaaacag cccccggacc gtggaattga
     3781 tggagacgga cgccctgacg acgcatgacg atccgttcag cctcatcgta tggaacgatg
     3841 aggtcaacac gttcgaatgg gtgatcgaaa cgctgataga agtatgcgga cattcggtcg
     3901 agcaggcaga acaatgtgcg atgatcatac acacaaaagg caagtatgcg gttaagcgtg
     3961 ggtcgtatga tgaattgaag cctgtatgcg atgccattac cgaccgcggc atcggcgcca
     4021 ccatagagat cctctccggt tcatgaacaa ccatacgcag gggcgccaac catgtcgcta
     4081 ttcatcttaa atcatgagct tatttttccg ccggtgcagc tggcggaacc cgacggcttg
     4141 ctggccgcgg gaggcgatct ttctacggaa agactgttgc tggcataccg gcagggcatc
     4201 tttccctggt atgagggacg ccatatcctg tggtggtgtc ccgatccgcg attcgtcctt
     4261 ttccccggcg aactcaaaga aagcaagagc atgaaacagt tgctcaggcg ggaagccttc
     4321 gatttccgga cagataccgc tttcggcgaa gtgatctcca attgcaagac tatcgcgcgc
     4381 aaaggccagg aaagtacctg gatcaccgat gaagtaagat cagcgtatac gcgtcttcac
     4441 ggtgccggat atgcgcacag tgcggagaca tggtacgaag gccagctcgt cggcgggtta
     4501 tacggcgtgc gtatgggcag ggtcttcttc ggtgaaagta tgttcagcag agccagcaat
     4561 gccagcaagt atgcattcat acgttatgtg catcaactcc gcgatgaagg cgtggagctc
     4621 atcgattgcc aggtctacac cgaacacctc gaatcgctcg gcgcgaggat gatccccagg
     4681 ggaaatttta tatccttgct ggacagcctc atcaaataaa cttttagccc ggatatacga
     4741 agagatacct cttagcgtta taacagtatc caatacttat tttttggacc agtaacccta
     4801 acaaaaacca aaaacatgaa gaaattccta ttggcagcga tctgtctgcc tgcactgacc
     4861 attctccacg cacaagacct gaccgttacg aaagcacctt cgatgtcgat gcacaagtac
     4921 gctcctttta aattagattt ttctacaggt ttcgctatcc cgtcgggatc cggcgcgaaa
     4981 gcaggggtgt ccttcgctct cgagcccaag tatgcaatta tagataagtt cgctgttggt
     5041 cttcggctgg aagcggctat tactgcccgt ggctgggtag ccagtgatgg ttcttcggca
     5101 agcgccagtg tggccgccag cgcttcctat ctcgcaacat atgattatta tctgcccgga
     5161 ctcatcttcc gcccgtttgt aggcggcggc acgggtgtct acaacatggc aagcgcttcg
     5221 gtggccgcgg acaaccaggc caacacgagc gtcgggacga gcgcatcgac taaggtcggt
     5281 accatgttcc gcaccggttt tgaatttcac catttccgga tggccttcga atataacgtt
     5341 atcggaaaga ccacgcagac ggtcaccgat ggcagcggta atacggtggg aaccatctcc
     5401 gcgaagaaca gctatgcgaa cattaagatc ggcttcttct tcggcggtgg caaacgctaa
     5461 tcaacctcga tctcttttca gccggaaacc gttatgggtt ccggcttttt tttacagctg
     5521 tatcatgatc ttcttggccg gccacctctc tacataagag tagatcaacc cccgcatata
     5581 atcgttctcc gtacagggag tgagatattg cttgagctcc gaggcatcat agatcgtcgc
     5641 atccgcgggc agatagccca taccgtagac ccggccctgt tctaccagga tacacgattt
     5701 ttcttcgcca tgacgtcccc tgtccatcac ggtgaagctc ggaaggcttt cgagcagcgt
     5761 ggctatcgcc gcccttactt ttccattata ggtttccggc gactcccgat gttcgcacgc
     5821 gccgtcgcag gtttcttccc gcataccttc gcagccgtcg ttgtcccgct ggataaaaca
     5881 aagcttggga catagccgga actctcggat cagcttgcgg aggatgttcc ggccctccgc
     5941 gagcatcgaa aaagtataga cgggccgcag ctgccgtttg cggcgttcga ggatcagccg
     6001 ggaatagccg ttaaggtctt catagacata gagcccgtaa gtggcctcaa accgtttcat
     6061 actccggttg tacgctggcc agagacgttt gatctcgatg cattccagca aaaatgccat
     6121 cagctcggtt cctgtcgtct catgcgagat cgaatagatg ttgcggagga attcctgttt
     6181 ctgccgaccc gtcttgttat tggagaaatg gctggcgacc ctatgacgaa ggttcttggc
     6241 cttcccgaca tagatcacct ttcctttctg atcgtgaaaa taatataccc ccggcacctg
     6301 cggcagtatt tccagctgcc cggcgggaag atgaatgggc aggtattgct cgctgttcct
     6361 ccctttgagc atgccgcgga tgatctcgag gccacccgac tgaaggatct tttcgaacag
     6421 gagagtggtg gcatccgcgt cccctccggc cctgtgccgg ttggaaatat cgatgcccag
     6481 gtaacggcag atattgccaa ggctgtatcg ctgcgcctcc gggaagacct tgcggctaag
     6541 ccttaccgtg caaagttttt tgctattgag ctcgaacccg caggcctgta actggtgccg
     6601 gagaaaagaa tagtcgaaat tgacgttgtg tgcgacgaaa acggcatctc tcagccattc
     6661 agagacgacg ggagcgatga tttcgaagtc gggagcatcg gcgaccattt catccgtgat
     6721 cccggtgagc gcctggacat aacgggggat cagcataccc ggccggacca gtgtctcgta
     6781 ccgtttgagc tcccggttcc catcgtgtaa cacaatagca acttccgtta tagcgttatt
     6841 agcggcataa ccaccggtag tttctatgtc gactatcgca tacattctta ctatcaaaat
     6901 tagtaagaat cgttcgttag tagatgtccg ggccctgaaa aaccgttccg taccccaaga
     6961 aagcgatgag gt
//
LOCUS       DASYSV010000500         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2280171, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000500 DASYSV010000000
VERSION     DASYSV010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2280171"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /gene="nhaA"
                     /locus_tag="VGQ51_15445"
     CDS             complement(<1..876)
                     /gene="nhaA"
                     /locus_tag="VGQ51_15445"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEV9038029.1"
                     /translation="MASLAPSSMPLRHRFMTPLRDFLNDSRSTGVLLIGCTLVSLVIA
                     NSVGSGWYTGGWKTPLAGLATLHLPATSIEWINNFLMAFFFLLAGMEIKRELLNGELS
                     SFKKAILPFGAAFGGMLFPALIYLAFNFHSPTGHGWGIPTATDIAFSVGIASLLGKRF
                     PVGLKILLLALAIIDDLGAIIVIALFYGGHIHALWLATAGLVYGILLACNFLRIRFGA
                     LQIALGIALWYLMLQSGVEASITGVLFAFAIPVKDLAIIEKFIHKPVNFFILPLFALA
                     NTAILIPGDITGALST"
     gene            complement(1129..2694)
                     /gene="amt"
                     /locus_tag="VGQ51_15450"
     CDS             complement(1129..2694)
                     /gene="amt"
                     /locus_tag="VGQ51_15450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEV9038030.1"
                     /translation="MGLKLLHKIKDRSFIFAAIALGLLLTAVTPLPSIAQDPNGTQTG
                     TIKDLGVDTLAATKDTAMNKVISTVNTIGNADGHNKIAINIMWTLITGFLVMFMQAGF
                     AMVETGFTQKKNVAHTMAMNFMIYAIGVLGFWICGFAIMFGGGLNVASLGGAAGVTSS
                     EFTVHLFGHDFGLWGKAGYFLNSNVYDVGIFTLFLFQLVFMDTTATIPTGSMAERWKW
                     KSFVIYGFFISMFVYPIFGNWVWGGGWLSQLGVNFGLGHGHVDFAGSSVVHEVGGVAA
                     LAGALVIGPRIGKYGKDGRVNVIPGHNIPMAIIGTFILAFGWFGFNPGSTLAGTDLRI
                     SVVAVNTMLASASGAFLAMVYVWTKYGKPDPGMLANGMLAGLVAITAPCAFVNATSAI
                     IIGAISGILVVVVYNFLDSKLKIDDPVGAFAVHGANGLWGVLSLGLFADGKYGDGWNG
                     VPGTVKGLFYGDAKQFVAELIGGLTCFVFVFVVMYLFFKIIKGTRIGLRVPEQEEIAG
                     LDIPEMGVHGYVD"
     gene            complement(2757..>3529)
                     /locus_tag="VGQ51_15455"
     CDS             complement(2757..>3529)
                     /locus_tag="VGQ51_15455"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV9038031.1"
                     /translation="SGGGGILQAVKQLYISYSPASWIKFTGGTWATHIGYEVVDAYAN
                     RNYSMSYMFTNGPFSHTGLRADFTSGKNGFMIGVSNPTDYRIVPAGGQNNKNLIAQYS
                     YTAGDNFKVYLNYVGGRDIVDNKVHQYDLVLNTKISGTLGIGANGTVNVSSAKIKDSY
                     DPNKTWYGAAVYLLLDPKPWFGLTLREEYFSDKDGVNISGGANIFASTLTASFKVDGF
                     TFMPEFRLDNANNEIFSKHDGTTTKTDACFLIAAIYSF"
BASE COUNT          870 a          825 c         1013 g          821 t
ORIGIN      
        1 agtgctcagc gcaccggtga tatcgcccgg tatcaggatg gccgtattgg caagggcgaa
       61 gagcggcagg atgaagaagt tgacgggttt gtgaatgaat ttttcaatga tggcgaggtc
      121 ctttaccgga atggcaaaag cgaagaggac gccggtgatc gaagcttcca cgcccgattg
      181 cagcatcaaa taccagaggg caattccaag ggcgatctga agggcgccga atcttattct
      241 caggaaattg caggcgagga gaatgccata gacgaggccg gcggttgcca gccagagggc
      301 gtgaatatgt ccgccgtaga acagggcaat tacgatgatg gcgccgagat catcaatgat
      361 cgcgagtgcg agcaggagta tttttagtcc aacggggaag cgtttgccga gcagggaagc
      421 gataccgacg gaaaaggcga tatccgtagc tgtcggaatg ccccatccgt gcccggtggg
      481 ggaatgaaag ttaaaagcaa gatagatcaa tgccgggaag agcatgcctc cgaaggcggc
      541 gccgaatggg agaatggctt ttttgaaaga ggagagttct ccgttcagca gttccctttt
      601 gatctccatt cccgccagca ggaagaaaaa ggccatgaga aaattattga tccattcgat
      661 ggaggtagcg ggaagatgta gtgtcgcaag gccggcaagg ggtgttttcc atccgccggt
      721 gtaccaaccg ctgccgaccg aattggcaat gaccagcgag accagtgtgc aaccgatgag
      781 gaggacgccg gtcgagcggc tatcgttgag gaagtcgcga aggggcgtca tgaacctgtg
      841 gcggagcggc atggaagaag gagcaagact ggccattgga aaggttatat tagtaaaaaa
      901 taaaatctga tatttattta tgacctcatg ttaaatttac ggaaaagttg gctgtttcat
      961 acattttaat tgtaaaatta gagaaataaa tttacatatt tatttttgtt atttgaatga
     1021 taattttcga gatattaagt tgatattaaa gacttgatat agcgattcgt aaaaaaatgg
     1081 cggcattttc cttgccacca ttgttaacag attttttgcg atcgatctct agtccacgta
     1141 gccgtgaacg cccatttcgg gtatgtcgag accggctatc tcttcctgtt ctggcacccg
     1201 taatccgatc ctggtgcctt ttatgatctt gaaaaagaga tacatcacga cgaagacgaa
     1261 aacaaagcag gtgaggcctc cgatcagttc ggccacgaat tgtttggcat cgccgtaaaa
     1321 gagacctttg acggtaccgg gtacgccatt ccagccatcg ccgtatttac catctgcaaa
     1381 aagaccgagg gatagcacgc cccacaggcc attggctccg tggaccgcga aggcgcctac
     1441 cgggtcatcg atctttagtt tgctgtccag gaagttgtaa acgaccacta ccagtatgcc
     1501 tgagatggcg ccgataatga tcgccgaagt tgcattgacg aatgcgcagg gtgcggtgat
     1561 ggccaccagt cctgccagca tgccgttggc caacataccg gggtccggtt tgccatactt
     1621 cgtccacaca tataccatcg ccaggaatgc gccgctagcg ctggccagca tcgtgttgac
     1681 cgcgactacg gagatgcgaa gatcggtgcc ggcgagggta gatccaggat taaaaccgaa
     1741 ccatccgaag gccaggatga aggtcccgat gatcgccatg ggaatattgt gtccaggtat
     1801 aacattgacg cggccgtcct ttccgtattt accgatgcgt ggtccgatga ccagggcacc
     1861 ggcgagcgct gctacgccgc ccacttcatg tacgacggaa gagccggcaa agtctacgtg
     1921 tccgtgcccc agcccgaagt tgacgcccaa ttgggacaac catccgccgc cccagaccca
     1981 gttcccgaag atgggataga cgaacataga aataaagaat ccatatatca cgaaggattt
     2041 ccatttccat ctttccgcca tggagccggt ggggatggtg gcggtggtat ccatgaatac
     2101 gagctgaaaa aggaagaggg tgaagatccc tacatcatat acattgctat tgaggaaata
     2161 accagccttg ccccacaggc caaagtcgtg tccgaacagg tgaacggtga actcggagct
     2221 ggtcacgccg gccgcaccgc cgaggcttgc gacgttgagg cccccgccga acatgatggc
     2281 gaagccgcag atccagaatc ccagcacgcc gatggcatag atcatgaaat tcatagccat
     2341 tgtatgagcc acgttctttt tttgcgtaaa gccagtctcg accatcgcga agcctgcctg
     2401 catgaacatg acgaggaagc cggtgatcag ggtccacatg atattgatcg cgatcttatt
     2461 gtgaccgtcg gcattcccga tcgtgttaac ggtcgaaatg accttgttca tagccgtgtc
     2521 tttggtggcg gccagtgtat caacgccaag gtctttgatc gtaccggtct gtgtgccgtt
     2581 ggggtcctgc gcgattgaag gtaaaggggt caccgcagtg agcaggagtc cgagggcgat
     2641 ggctgcgaaa atgaaagaac ggtccttgat cttgtgcaac agttttaatc ccattgtttc
     2701 gttgttttcg gttatgtatt ttttttgacg tggatcaggt aatggtcaat attggatcaa
     2761 aaggaataaa ttgccgcgat gaggaaacag gcgtccgttt tggtggttgt gccgtcatgt
     2821 ttgctgaaga tctcgttgtt tgcgttgtcg aggcggaact ccggcatgaa ggtgaagccg
     2881 tcgactttga agctggctgt gagcgtggag gcgaagatat tggcgccccc ggagatattc
     2941 acaccgtctt tatcgctaaa gtactcttcg cgcagggtga ggccgaacca gggtttggga
     3001 tcgagcaaga gatagaccgc ggcgccgtac caggtcttgt tcgggtcata ggaatctttg
     3061 atcttcgcgc tggagacgtt gaccgtgccg ttggcgccga tgcctaatgt gccggatatt
     3121 ttggtattga ggacgagatc gtactgatgg accttattgt cgacgatgtc ccttccgccg
     3181 acgtaattga gatagacctt gaaattatcg ccggccgtat agctatattg cgcgatcagg
     3241 ttcttgttgt tttgtccgcc tgcaggaacg atcctgtaat ccgttgggtt ggagacgccg
     3301 atcatgaagc cattctttcc cgaggtgaag tccgcgcgga ggccggtgtg agagaagggg
     3361 ccattcgtga acatatacga catgctataa ttgcggtttg cataggcgtc gactacctca
     3421 tagccgatgt gggtagccca ggtgccgccg gtgaacttga tccaggaagc gggagagtaa
     3481 ctaatgtaca attgttttac cgcctgtaat atgccaccgc cgccgctgc
//
LOCUS       DASYSV010000501        19226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2149951, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000501 DASYSV010000000
VERSION     DASYSV010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 19226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19226
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149951"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1571
                     /locus_tag="VGQ51_15460"
     CDS             159..1571
                     /locus_tag="VGQ51_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HEV9038032.1"
                     /translation="MNEKVSIGVIIGNRDFFPDKLVSEARRDILGQFDKLGLNAILPE
                     ESDTKLGGVETFADARKCAALFSKHADTIVGILVILPNFGDEKGVAETIKLSGLNVPV
                     LIQAYPDELNKLDVARRRDSWCGKISVCNNLYQYGIRYTLTGKHVLHPKDPGFETELK
                     DFVAICRVVRGLRKVRIGAVGARPGAFNTVRYSEKILQRNGISVTTVDLSEILGRAGR
                     LTKDDAPVKAHLDKILAYAPKGKTPNEAMLQIAKLDVILNQFMEEHYLDATAIQCWTS
                     LQQNFGCNVCTSMSIMSENMLPSACEVDVTGTLSMYAMQLASGSPSALVDWNNNYADD
                     DTKCVLFHCGNWAKSFLPDIQISNAPIIGTTVGVENTYGALDGRTPANPLTYGRISTD
                     DPKGIIKAYVGEGVLTDDPLNTFGNRAVAQIADLQGLMQYVCRNGFEHHVVMNASKTA
                     RILEEALGNYMGWELYHHKS"
     gene            1575..2393
                     /locus_tag="VGQ51_15465"
     CDS             1575..2393
                     /locus_tag="VGQ51_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV9038033.1"
                     /translation="MTDNELAKRSTQYRRNILKYIVRAGAGHTGGSLSCIDILNVLYN
                     HTLNVNPENFRSPDRDRYIQSKGHCVEALFVVLADKGFFPESDLNTLCKYRSHYIGHP
                     TRKVNGVEQNTGALGHGLPIAVGTALAAKMDKKDYRVFTLMGDGELPEGSNWEAALTA
                     AHYKLDNLCAIIDYNGLQITGPTADVCNTDPIDRKFSSFGWAVTHVDGHDLKALQTTF
                     DSLPFEKDRPSLVIAHTVKGKGISFMENTLSWHHGVPNQEQYTLALKELGDAVI"
     gene            2405..3373
                     /locus_tag="VGQ51_15470"
     CDS             2405..3373
                     /locus_tag="VGQ51_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV9038034.1"
                     /translation="MASIVVESKPGAKPNLEVFSATLQELAATDRDIVVVTSDSRGSG
                     KLVPFGKKFPDQIVEIGIAEQNLVGVAAGLASAGKKTFAVSPACFLTARALEQIKNDV
                     AYSDNPVKLIGISAGVSYGALGSTHHSLHDFAALRAINNLIVAAPADNFETEEAIRQA
                     AASSHPIYLRFGKKNMPLLTTDRGNTFRFGKGRVITKGGDLAFIATGETVQPALEAAA
                     VLREAHGVEATVISMHTIKPLDTTLLRETAATTAAIITVEEHSIYGGLGEACASYLLQ
                     YGYGKPFRIVGIPDEYTVTGSQNEIFDHYGISGEGLTATALDLLGK"
     gene            3382..4323
                     /locus_tag="VGQ51_15475"
     CDS             3382..4323
                     /locus_tag="VGQ51_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV9038035.1"
                     /translation="MKKLTIALLSLSLMLAACSPGDDHHAPKKIAIVVSTLNNPWFVY
                     LAQTAAAKAKALGYESKIFDSQNNTALESDHFDNAIASGYNAILFNPTDAKGSVINVK
                     KAKDAGIPVFCMDREIDSKDAATSQILSDSYSGCIDLGKLFVRALNKKGNYVELLGIV
                     SDDNTWNRSKGFHSVVDNYPQLKMVAQQSADFDRNKAMEVMESILQAHPDIDGVFAGN
                     DAMAMGAWQALTAAGLAGKVKVIGFDGAEDAINSIKDGKLTATAMQFPKIIAELAATD
                     ADEYFRGKRDFPHKLPIPVDVVSQANIDKYTAYGKKN"
     gene            4327..4947
                     /locus_tag="VGQ51_15480"
     CDS             4327..4947
                     /locus_tag="VGQ51_15480"
                     /inference="COORDINATES: protein motif:HMM:NF021565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 domain-containing protein"
                     /protein_id="HEV9038036.1"
                     /translation="MSSPIKYTLLVFAIALVGYNSIYTRKLSDVQKEARAEDFDAKAY
                     AENFLHATLPAHPDKAIDLGQLAQTLTSDPAKAFSWSHAQNNGNNRFFLVKGSGKITK
                     IDSESVTVAVEGVTQKVLLATRYIIGTAARDGSGLISVDEFTTTMDMNTVSEELNKLI
                     RDQVLPPFRTAAKVGDNVRLTGGLELQQNAASPDTLEIIPMILTIQ"
     gene            4965..6461
                     /locus_tag="VGQ51_15485"
     CDS             4965..6461
                     /locus_tag="VGQ51_15485"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEV9038037.1"
                     /translation="MLVAEKIGKRFAGVIALRDVSMQLRPASVTAIIGENGAGKSTLM
                     KILSGVYSDYEGRILLNGEEVRFANPRDAQEKGISIIHQELNLIPDLSITENIFLGRE
                     LTDRWGLLNKKAMRERVTALLQRLRLNVSPDSLIRDLRVGQQQVVEIAKALLLESRVI
                     IMDEPTSAISETEVDILFGIIEQLRAEGKIIAYISHKLDELFRIADDFVVLRDGQVTD
                     TGTMQSVDHDAIVSKMVGREITRLEHTRTQSETGELLSVTTLNWKTGAKQILRDISFS
                     LHRGEVLGIFGLMGAGRTELLECILGLHAGDYTGELKIEGRLSDIRTPRDAIRAGIVL
                     VPEDRKKDGLIPGLDVRTNISLSVLRSIGRNGLVNGKAERTLAMHYKDSLRIKTPNLE
                     QPVRHLSGGNQQKVILARCLATNPKVLMLDEPTRGIDVNAKNEIYRLILELAEGGLGI
                     IVVSSELPEILAVSDRILVLCEGKLTGVFPRATANEDDILKAAIPSTT"
     gene            6472..7446
                     /gene="rbsC"
                     /locus_tag="VGQ51_15490"
     CDS             6472..7446
                     /gene="rbsC"
                     /locus_tag="VGQ51_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216384.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEV9038038.1"
                     /translation="MVLSKYRPLLTRFQSFIALLILCLALSLLSNKFLTIENGWNVLR
                     QISVNVCISVGMTLVVLTAGIDLSVGSVLAFSGAVAAGLWKNGIPWPAHDLFIGFTLL
                     GTIVAALLMGGALGFFNGWMITKFRVPPFVATLAMLTIARGLTMLWTKGYPISGLGAK
                     FDHIGSGWLLGIPVLVWISAAAVILALILVKKTPLGRYIYAIGGNEQAARLSGIHVHR
                     VKIIVYTLGGILAAVGGIMVTSRLDSAQPNAGTNYELDSIAAVVIGGTSLSGGRGTIM
                     GTVLGAIIIGVLNNGLVLLDVSPFWQQVIKGVVILLSVIVDKMNSKEA"
     gene            7443..8933
                     /gene="glpK"
                     /locus_tag="VGQ51_15495"
     CDS             7443..8933
                     /gene="glpK"
                     /locus_tag="VGQ51_15495"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV9038039.1"
                     /translation="MNYILGIDQGTSGTKALVFDEHGKPVAKGAETLKTSYPENGHVE
                     QDPEGILRNVLTAVAQCLETFASGGGRPDDIRACGISNQRETFVLWDRSGTPLCNAIV
                     WQCKRSVDICNRWRSAGYEPLIRERTGLLIDPYFSGSKLVWLYENIPAVRSKIDAGEA
                     CFGTIDTWLLYRLTNGHSFYTDYTNASRTLFFNLKELRWDRELLELFGLGRLQLPGLG
                     PSSGSFGGTDFNGLLPSPLPITGVIGDSHAAAFGEGCFSPGEAKATLGTGCSILMNTG
                     RTCMDSRNGMVSTICWSTADAVHYALEGVIVTCGATLEWLKNELNLFTDSRQTLEMAN
                     AVQDNGGVYLIPAFSGLGAPHWEMDRKASITGLTFGTQKNHIVRAALESIPYQIKDVI
                     VAMEENSSLDLSRLMVDGGISANPFVTQFLADLLEKEVVTIGQPDISALGAAYMSGLS
                     AGIYPGIDYLCSLNTEKKIITPAPGSKAAKYYAGWLETLSAGRCRQ"
     gene            complement(8885..10135)
                     /locus_tag="VGQ51_15500"
     CDS             complement(8885..10135)
                     /locus_tag="VGQ51_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEV9038040.1"
                     /translation="MLRFRMICLWCSGIALPVLIAGLCLSNGGLWQPAALALSVCLAI
                     GMGAVPRLAGYQYTAWIVTAVVAGMLYPAAFSHWGSLDLRNKWLVLIVVQLVMFGMGT
                     QMRLRDFTGLAGTGKGVLIGLACHFSVMPLTGYLLTRVFHFDPEIAAGIILIGSCSSG
                     LASNVMVYIARANLTLSVAVTAMATLAAPFFTPLLMKLLAGTYIHVQFGDMMLEIVKI
                     VLVPIGAALLHDYLNGASVKGRRWTLATGAVAAVWLVVLGIGRIDGGITGFFAGAILA
                     GIGYHYLVRGWPAVQRVMPYISMAGIVYFTTVTTAAGRDNLLHVGLLLFLASVFHNAA
                     GYFFGYWLSRLFRLDKNAARTVAFEVGLQNGGMASGLAGSMGKLGTVGLAAAVFSPWM
                     NISGSILANYWRHRPADKVSSQPA"
     gene            complement(10143..10799)
                     /locus_tag="VGQ51_15505"
     CDS             complement(10143..10799)
                     /locus_tag="VGQ51_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEV9038041.1"
                     /translation="MQKTLGLIHTSATLVPVFQQLTSKGLPGVKVFNIVDDSLIADVI
                     RKGQLTSQTARRVVDYVGSAENAGADYIMVTCSSIGAAVEASAALTRVPVLRVDQPMA
                     DLAVAKGTRVGVIATLPTTLAPTSDLVKRRAAIAGKAIELTSRLCEGAFDALMGGDPA
                     KHDKMVADALRELSAQVDVIVLAQASMARVVDALPEADKRIPILASPPTAIEYLKSVL
                     "
     gene            complement(10804..12090)
                     /locus_tag="VGQ51_15510"
     CDS             complement(10804..12090)
                     /locus_tag="VGQ51_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEV9038042.1"
                     /translation="MSLLLAFYGDDFTGSTDALEFLSRAGARTVLFMEPPGAEQLKRY
                     PDLEAIGIAGMTRAMPPDEMEAVLRPAFGVLAGLGVPHVHYKVCSTFDSSPAIGSIGK
                     AIDIGADIFRAGFVPVLGGAPALGRYCVFGNLFARMGIGSEGNIYRLDRHPSMSKHPV
                     TPADESDLRVHLGRQTSRNIGLLDIVGLEMGPNEQRKILERSAVTLIDVLYATQLERI
                     GALLDGYGSPGQPLFSVGSSAVEMALGDHWKADGRLTPPAEWPSPGRAEPLLVVSGSC
                     SPVTAGQIAWAKANGFAGIALDAAAVAEWGSHVKEAREYLKEGRSLILHTGHTPAGPI
                     PAALLGTALGKLVKEILAGWDGVRRLVIAGGDTSGYAARALGIGAVEMICPLIPGAPL
                     CRAYAPGSTVDGMEINLKGGQVGGEDYFGKLLNGKK"
     gene            complement(12087..13334)
                     /locus_tag="VGQ51_15515"
     CDS             complement(12087..13334)
                     /locus_tag="VGQ51_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit
                     family protein"
                     /protein_id="HEV9038043.1"
                     /translation="MERITALYHIETPLRPAAAAAILAGEQSSGTFVAVPGETEELKT
                     RFAAQVESVEQLETVEHPAIPGGAGFQKGPFHRAAIRVSWSIENCGYNLPVLISTVQG
                     NLYELSAFTGLKLMDLDIPPSFAQHFGGPVFGIEGCRRLTGVYDRPLIGTIIKPSIGM
                     TPEQTADLVKTLAGAGIDFIKDDELLSAAANSSFEDRVAAVMKVVDEHADKTGKKVMY
                     AFNLSAEIDTMLRRYETLVKAGATCAMVSLNSVGLSGVKKLCDMRQLAIHGHRNGWGM
                     LNRHPLLGIEFPAYQKLWRLAGVDQIHVNGIQNKFWESDDSVVRSIDACLTPFLGGYS
                     ILPVVSSGQWGGQAPETYRRTKTIDLLYMAGGGIMAHPDGPAGGVTALRQAWQAAVEG
                     LSVEEGARKYPAFAKSVQKFGRK"
     gene            13454..13786
                     /locus_tag="VGQ51_15520"
     CDS             13454..13786
                     /locus_tag="VGQ51_15520"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV9038044.1"
                     /translation="MKGTHLGEFEELVLLTIASLVNSAYSVAICDELSKHADRIVKLG
                     VVHAVLHRLEEKALIKSELGEASATRGGKRKRFYTLTTAGKASLLRSKEIRDTLWQKI
                     PNLTLNRI"
     gene            13786..16509
                     /locus_tag="VGQ51_15525"
     CDS             13786..16509
                     /locus_tag="VGQ51_15525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038045.1"
                     /translation="MKEKMPEPPKWATRFLTWYCRPTLLEDLQGDLNEYFHRNVRERG
                     ASSARLIYIIDVLKFFRPYIIRKPSFSNPLKGKITMKKQLLFTLRRLSRHKLTAGINI
                     LGLTLGILSCLVIFLYVSFEFSYDKFHADAGRIYRVIVSAAKKNGSHGSADMTSPLGP
                     DLRRETSAFSAVTSLHTDDTRVLVPITGKPARVIPTIGDDERQHITFADSDYLKIFHY
                     RWLAGNPATALQKPFSVVLTESEAQRYFQGGQPEDWMGRSIVYYDSLPVAVTGIVKDW
                     DHNSDLGFRDLISYNTIEHSFLQKDMEGWNLWAGNVNVYVKLAPGTTPAQAEQQLPSF
                     LQRHIQANGKAVLSLQPLADIHFNGEYADRYGRRAHKPTLYALTGIALFILVIAAINF
                     INLSTAQSILRAREVGVRKVLGSSRGSLVRQFLNETGIIVTSAMVLALLLANPTIRAL
                     HGFIPEGVRLHIGDVYTWIFAGSTILVTCLLAGWYPARAISSFLPVITLRGQGVQQLN
                     SKSYLRKSLIVFQFTVSLIFIIGTMMVGRQIRYMLNTDLGFKKDAIVTIGLPWDQPKD
                     RKIVLATGIRRLAGVQEVSVNTGSPEAASHGGTGIEYNGKTHTKIDAGFVQIDPDYIP
                     LYELALVAGRNFTIADTARRGSDPASGDPGPAQTPFRAFILNETAARALGFNNPADAV
                     GQQVSSGFSGIPGPVVGVVRDFHSQNLHEKIQPFFFTEQRAAGGTLSIKLSSATLPAV
                     QVKTLMTELETSFKKIYPASVFQARFFDESLARLYRQEQQTAEIMNIAMGIAIFISCM
                     GLFGLAAFTANQRTREIGIRKVLGANVPQLVSMLSRQFILLVMLSTLIAAPIAGWGMH
                     MWLEDFAYQTSMPWWIYVVAGAVAVAIALVTVSFQAIRAAMANPVRSLRSE"
     gene            complement(16556..16810)
                     /locus_tag="VGQ51_15530"
     CDS             complement(16556..16810)
                     /locus_tag="VGQ51_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV9038046.1"
                     /translation="MRMTISGLPRHYDKFDLQRLFNPFGWVDYSKILIDPITGLSRGK
                     GIVEMKRDEEARKAMEALQGKVLGANPLNIKEAKERGPKP"
     gene            complement(17839..17909)
                     /locus_tag="VGQ51_15535"
     tRNA            complement(17839..17909)
                     /locus_tag="VGQ51_15535"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17875..17877),aa:Cys,seq:gca)
     gene            18017..18892
                     /locus_tag="VGQ51_15540"
     CDS             18017..18892
                     /locus_tag="VGQ51_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV9038047.1"
                     /translation="MQPIPDSELIINSRGALYHLDLRPEELAPIVLTVGDPGRVEKVS
                     RYFDSVEHRSSHREFISHTGRIGQKRLTVVSTGIGTDNIDIVLNELDALVNIDLDTRM
                     VKRQFTSLTIIRMGTAGALQKDIPADEFVASTHGLGIDNLLNFYRHDISEEENELLHS
                     FSTQTQLHHRLAQPYISSASGSLLRHFTTGFHHGITVTCPGFYGPQGRVLRLGLTQPE
                     LIDRLTGFTYGPHRIANFEMETAGIYGLGKLLGHSCLSLSAIVANRVLKTFSRDGDAT
                     VDRLIRGTLRIVEGL"
     gene            18934..>19226
                     /locus_tag="VGQ51_15545"
     CDS             18934..>19226
                     /locus_tag="VGQ51_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEV9038048.1"
                     /translation="MSQLHFFKYQGTGNDFVILDNRNWSYTALTTERIRFLCDRRFGI
                     GADGLMLLNPHSGYDFEMKYYNADGRESTMCGNGGRCLVKFAYDLGIRKNEYS"
BASE COUNT         4569 a         5910 c         4823 g         3924 t
ORIGIN      
        1 gccgacagca ccaagatcgg gaaaagctct ttcgccagcc ttggcgcgct gtccctgatc
       61 aactacgtga tcaccgaccc cggtatcagc gaacgcgata gacgcatctt tgaagacaac
      121 gagatcggac tgatcctcgc caactaaata aatcaaacat gaacgaaaag gtcagcatag
      181 gagttattat cggcaatcgg gatttcttcc ccgacaaatt agtctcggaa gcccgccggg
      241 acatccttgg acaatttgac aaattgggac tgaatgccat ccttcccgaa gaatcagata
      301 ccaaattagg tggcgtggaa acgtttgccg atgcccgcaa atgcgccgcg ctgttttcca
      361 aacacgccga cacgatcgta ggcattctgg tgatcctgcc gaatttcggc gacgaaaaag
      421 gtgtagcaga aacgatcaag ctctccggac tcaatgtccc cgtgctgatc caggcctacc
      481 ccgacgagct caacaaactg gatgtagccc gccgccgcga ttcctggtgc ggcaagatct
      541 ccgtttgcaa caacctgtat caatacggta tccggtatac gctcacgggc aaacacgtgc
      601 tacacccaaa agacccgggc ttcgaaacag agcttaaaga cttcgtcgcc atctgccgcg
      661 tcgtccgcgg tcttcgcaag gtccgtatcg gcgccgtcgg cgcccgcccc ggcgctttca
      721 acacagttcg atatagcgaa aagatcctcc aacgcaacgg catttccgtg accaccgtcg
      781 atctttccga gatcctcggc cgtgcaggcc ggctcaccaa agacgatgcg ccggtaaagg
      841 cccatctcga caagatcctg gcctatgccc cgaaaggcaa gactccaaac gaagccatgc
      901 tccagatcgc gaaactcgac gtcatactca accagttcat ggaagaacat tatctcgacg
      961 ccaccgccat tcagtgctgg acctcactgc agcagaattt tggctgcaac gtctgcacca
     1021 gcatgagcat catgagcgag aacatgctgc ccagcgcctg cgaggtcgat gtcaccggca
     1081 cccttagcat gtatgcgatg cagctggctt cgggctcacc cagcgcactg gtagactgga
     1141 ataacaacta tgccgacgac gacaccaaat gcgtgctttt tcattgcggc aactgggcca
     1201 aatcattttt acccgacatc cagatcagca acgcacccat catcggcacg accgtgggcg
     1261 tagaaaatac ttacggagcg ctcgacggcc gcaccccggc caatcccctt acctatggcc
     1321 ggatcagcac cgatgatccg aaaggcatca tcaaagccta tgtcggcgaa ggcgtcctca
     1381 ccgacgatcc gctgaatacc ttcggcaacc gcgccgtcgc gcagatcgcc gatctgcagg
     1441 ggctgatgca atatgtctgc cgcaacgggt tcgaacacca cgtcgtcatg aatgcctcga
     1501 agacggcccg gatactcgaa gaagccctcg gcaactacat gggctgggaa ctgtaccatc
     1561 ataaatcgta aactatgaca gacaatgaac tcgcaaaaag atcgactcaa taccgccgaa
     1621 acatcctgaa gtacatcgtc agggcgggcg cgggtcacac gggcggaagc ctctcctgta
     1681 tcgatatcct caacgttctc tataaccaca ccctgaacgt aaacccggaa aatttcagat
     1741 cgcccgacag ggacaggtat atccaaagca aaggacattg tgtggaagcg ctctttgtag
     1801 tgctcgcgga caaaggattc tttcccgaat ccgatctgaa tacactctgc aaatacagat
     1861 cgcactatat cggtcacccc acacgcaaag tcaatggcgt agaacaaaac accggcgccc
     1921 tcggccatgg tctgccaatc gcggtaggca cggctctcgc agccaaaatg gacaaaaagg
     1981 actaccgtgt ctttacactg atgggcgacg gcgagctgcc cgaaggttcc aactgggaag
     2041 ctgcccttac cgccgcgcac tacaagctcg acaatctctg cgcgatcatc gactacaacg
     2101 gtctgcagat caccggtccc accgcagacg tttgcaatac cgaccccatc gacaggaaat
     2161 tctcaagctt tggctgggcc gttacccatg tcgatgggca tgatctcaaa gcgctgcaga
     2221 cgaccttcga cagtctgcct ttcgaaaaag acagacccag cctcgtgatc gcccataccg
     2281 taaaaggcaa agggatcagc ttcatggaga acacgctgag ctggcatcac ggggtaccca
     2341 accaggaaca atatacgctc gccctcaaag aactgggcga cgccgtcatt taaaaaacaa
     2401 gatcatggcc agtatagtcg tagaaagcaa acccggagca aaacccaatc tggaagtctt
     2461 ctccgccacc ttgcaggaac tggcggcgac ggacagggac atcgtcgtcg tcaccagcga
     2521 ttcccggggt tcgggtaagc tcgtcccttt cggaaaaaaa tttcccgacc agatcgtaga
     2581 gatcggcatc gccgaacaaa acctcgtggg cgtcgcggcc ggcctggcct ccgccggcaa
     2641 aaagaccttc gctgtatcgc ccgcatgctt tctcaccgcc cgggcgcttg agcagataaa
     2701 gaacgatgtc gcctattcgg ataacccggt gaagctcatc ggcatcagcg cgggcgtgag
     2761 ttatggggca ctgggttcca cccaccacag cctccacgat ttcgcagcgc tcagagccat
     2821 taacaacctc atagttgccg cccccgccga caacttcgaa accgaagagg cgatcagaca
     2881 ggccgcagca tcgtcacacc cgatctatct ccgttttggg aaaaagaata tgccgctcct
     2941 caccaccgat cggggcaata cctttcgttt cggtaagggg cgggtgatca caaaaggcgg
     3001 cgacctggcc tttatcgcta ccggcgagac cgtccagccc gcattggaag ccgccgccgt
     3061 cttgcgcgaa gcccatggcg tcgaagccac ggtgatcagc atgcacacga tcaaaccgct
     3121 ggacactacc ttgttgcgcg agacagccgc cacaacggca gcgatcatca cagtggaaga
     3181 acatagcatc tacggaggtc tcggcgaggc ctgcgcgtcc tatctgttgc aatatggata
     3241 cggcaagccc ttccgcattg tcggtatccc cgacgaatac accgtcaccg gttcgcaaaa
     3301 cgagatattc gatcactacg gcatttccgg cgaagggctt accgccaccg cactcgatct
     3361 tttaggtaaa tagtacaagc catgaaaaaa ttaacgattg cccttctatc gctaagcctg
     3421 atgctggcag cctgctcacc cggcgacgat catcatgcac ccaaaaagat cgctatcgtg
     3481 gtctcgaccc tcaataaccc atggtttgtc tatctcgccc aaaccgcggc cgctaaagca
     3541 aaagccctgg gctatgaatc gaagatcttc gactcgcaaa acaacaccgc gctggaaagc
     3601 gatcatttcg acaatgccat cgcttccggc tataacgcca tccttttcaa tcccaccgac
     3661 gctaaaggct ccgtgatcaa cgtcaaaaaa gcaaaagacg ccggcatccc cgttttctgt
     3721 atggatcgcg agatcgattc caaagacgcc gcaacctcac agatcctctc cgacagctac
     3781 tcgggatgta tcgacctcgg caagcttttc gtcagggcgc tgaacaaaaa aggcaactat
     3841 gtcgaactcc tgggcatcgt ctccgacgac aatacgtgga accgctcaaa aggttttcat
     3901 agtgtggtcg acaattatcc gcaactgaaa atggtcgcgc aacaaagtgc cgatttcgac
     3961 cggaacaaag cgatggaggt gatggaatcg atcctccagg cgcatccgga catcgatgga
     4021 gtgtttgctg gcaacgacgc catggccatg ggcgcctggc aggcgctcac cgccgcagga
     4081 ctggccggca aggtaaaggt catcggtttc gacggcgccg aagatgccat caattctatt
     4141 aaagacggga aactaacggc aaccgccatg caattcccaa agatcatcgc cgagctggcc
     4201 gctacagacg cagacgaata tttcaggggg aaacgcgatt ttccacacaa gctcccgatc
     4261 cctgtggacg tggtcagcca ggctaacatc gataaataca cggcatatgg caaaaagaat
     4321 taacccatga gctcaccgat aaaatatacc ttactcgtgt tcgcgatcgc tctcgtcgga
     4381 tataattcga tctatacccg aaaactcagc gacgtccaaa aggaggccag ggcggaagat
     4441 ttcgacgcaa aagcctatgc agaaaatttc ctgcatgcta cccttccggc acatccggac
     4501 aaggccatcg atctcgggca gcttgcccaa acgctgacaa gcgatcccgc caaagccttc
     4561 tcctggtcgc atgcccaaaa caacggcaac aaccgcttct tcctcgtcaa agggtccggc
     4621 aagatcacaa agatcgactc ggaatctgtt acggttgccg tcgaaggcgt cacacaaaaa
     4681 gtattgctgg cgacaaggta tatcatcggc accgctgccc gtgacggatc cgggctgatc
     4741 tccgtggacg agtttacgac gaccatggac atgaacaccg tctcggaaga gctcaataaa
     4801 cttatccggg accaggtcct gccgcccttc aggacagccg caaaagtcgg cgacaacgtc
     4861 cggcttaccg gaggtctcga gctgcaacaa aatgccgcct caccggatac ccttgaaata
     4921 atcccgatga tcctcaccat tcaataacgt taaacaatgc acatttgcta gttgcagaaa
     4981 agatcggaaa acgttttgcc ggagtgatcg ccctccggga tgtgtcgatg caattgcggc
     5041 cggccagcgt cactgctatc attggtgaga acggcgcggg taaatccacg ctgatgaaga
     5101 tcctttcggg cgtttactcc gattacgaag gtcgcatcct gcttaacggc gaagaggtcc
     5161 ggttcgccaa ccccagagat gcacaggaaa aaggcatctc catcattcac caggagctca
     5221 acctgatccc ggacctctcc atcaccgaaa acatcttcct cggccgcgag ctcacggacc
     5281 gatggggcct gctgaacaaa aaggccatgc gcgaacgtgt cacagcgctt ctgcaacggc
     5341 ttagactcaa cgtctccccg gacagcctga tccgcgatct cagagtcggc cagcaacagg
     5401 tggtggagat cgcaaaggcg cttctcctcg aatcccgggt catcatcatg gacgaaccca
     5461 cttccgccat cagcgaaacc gaggtggaca tccttttcgg tattatcgaa cagctgcgcg
     5521 ccgaagggaa gatcatcgcc tatatctctc acaagctgga tgaactgttc cgcattgccg
     5581 atgacttcgt ggtcctgcgg gacgggcagg tcacggatac aggtacgatg caatccgtcg
     5641 atcacgacgc catcgtcagc aaaatggtgg gccgcgagat cacaagactg gaacataccc
     5701 gcacgcaatc cgaaaccggc gaattgcttt cggtcaccac actgaactgg aaaaccggcg
     5761 ccaaacagat actcagggac atcagcttct cgctccaccg cggcgaggtc ctgggcatct
     5821 tcgggctcat gggtgcaggc cggaccgaat tgctggaatg tatcctcggt ctccacgcag
     5881 gcgactacac cggcgagctg aagatcgaag gcagattgtc cgacatccgg actccccgcg
     5941 acgctatacg tgccggtatc gtcctcgttc ccgaggaccg gaaaaaggac ggcctgatcc
     6001 ccggtctcga tgtcaggacc aatatcagcc tgagcgtcct ccggtccatc ggtcgcaacg
     6061 ggctggtgaa tggcaaagca gagagaacgc tggccatgca ttacaaagac tcgctccgga
     6121 taaaaacgcc aaacctcgaa cagcccgtcc ggcatctcag cggcggcaac cagcaaaagg
     6181 tcatcctggc ccgttgtctg gccaccaatc caaaggtgct gatgctcgac gagcccaccc
     6241 gcggtatcga cgtcaacgcg aaaaacgaga tctacaggct gatcctcgag ctcgcggaag
     6301 gcggtctggg tatcatcgtc gtctccagcg agctgcccga gatcctcgcc gtctccgatc
     6361 gcatcctcgt tctctgcgaa gggaagctga ccggcgtctt cccgcgcgcg acggccaacg
     6421 aggacgatat cctgaaagcc gccatcccat caacgacata aacctatgcc catggttcta
     6481 tcaaaatatc gccccttact cacccggttc cagagcttca tcgcgctgct gatcctttgc
     6541 ctggcactga gccttctgtc gaataaattc ctgacgatcg aaaacggctg gaacgtgctc
     6601 cggcagatct cggtgaatgt ttgcatttcc gtgggtatga ccctggtcgt tctcactgcc
     6661 gggatcgatc tttcggtggg ttccgtactc gccttcagcg gcgcagtcgc agcgggtttg
     6721 tggaagaatg gtatcccatg gcccgcacac gacctcttca tcgggttcac attattaggc
     6781 accatcgttg ccgctttgct catgggtggc gctctcggtt tcttcaatgg ctggatgatc
     6841 acaaaatttc gggtaccccc cttcgtggcc accctggcga tgcttaccat cgcccgcggt
     6901 cttacgatgt tatggaccaa aggatatccc atctcaggtc tgggcgccaa attcgatcat
     6961 atcggttccg gctggctgct cggcattcct gtcctcgttt ggatatccgc cgccgccgtg
     7021 atccttgcct tgatccttgt gaaaaaaaca ccgctcggcc ggtatatcta tgcgatcggg
     7081 ggcaacgaac aggcagcaag actctcgggt attcacgtac accgcgtaaa gatcatcgtt
     7141 tataccctcg gcgggatact cgcggcggtg ggagggatca tggtcaccag ccggctggat
     7201 tcagcgcagc ccaacgccgg caccaactat gagctcgatt cgatcgctgc cgtcgtcatc
     7261 ggcggcacct cgctctccgg gggacgcggc accatcatgg gcacagtgct cggcgccatc
     7321 atcatcgggg tgcttaacaa tggattggtg ctattggatg tgtcaccgtt ctggcaacag
     7381 gtgatcaaag gggtggtgat cctgttatcg gtgatcgtag ataaaatgaa ttcaaaagaa
     7441 gcatgaacta tatcttaggc atcgaccagg gcaccagcgg gaccaaggcg ttggtgttcg
     7501 acgaacacgg gaaaccagtc gccaaaggcg cggaaacatt aaaaacgtca tatcccgaaa
     7561 atggacatgt cgaacaggac ccggaaggga tcctgcgaaa tgtactgact gccgtcgcgc
     7621 aatgtcttga gacgtttgca tccgggggtg gccggcccga cgatatccgc gcctgcggca
     7681 tctccaatca gcgcgagacc ttcgtactct gggaccgctc agggacgccg ctttgcaatg
     7741 ctatcgtctg gcaatgcaaa cgctccgtcg acatctgcaa ccgctggcgg tccgcaggct
     7801 atgaacccct catccgggaa cgcaccggcc tcctcatcga tccctatttc tcgggttcga
     7861 agctcgtttg gctctacgag aacatccccg ccgtccgcag caagatcgat gccggcgagg
     7921 cctgcttcgg cacgatcgat acctggcttc tttacaggct caccaatggc cacagctttt
     7981 ataccgacta taccaacgca agcaggacgc tattcttcaa tctgaaggag ttgcgctggg
     8041 atcgcgagct gttagaactc tttggtctcg gcagacttca gctcccgggg ctggggccgt
     8101 cttccgggtc cttcggcggc accgatttca acggtctgct tccctcaccc cttcccatca
     8161 ccggtgtcat cggcgactct catgccgccg cattcggcga agggtgcttc tcgcccggcg
     8221 aagccaaggc caccctcggc accggctgct cgatcctcat gaataccgga aggacctgca
     8281 tggattcgcg caacggcatg gtctcgacca tctgctggag cacggcggat gccgtccact
     8341 acgccctcga aggagtgatc gtcacctgcg gcgcgacact cgaatggttg aagaatgaat
     8401 tgaatttgtt cacggacagc cggcagaccc tggagatggc caatgcggta caagataacg
     8461 gcggcgtata cctgataccg gcctttagcg ggctcggcgc cccgcattgg gaaatggacc
     8521 gaaaggcttc gatcaccggc ctcaccttcg gcacccaaaa gaaccacatc gtccgggccg
     8581 ccctcgaatc gatcccttat cagataaaag acgtgatcgt agcaatggag gagaattcat
     8641 ctctcgatct atcccgtctg atggtcgatg gcggcatttc cgccaacccg ttcgtcaccc
     8701 aattccttgc cgacctgctg gagaaagaag tcgtcaccat cggccagccc gacatttccg
     8761 ccctcggcgc cgcctatatg tcgggactga gcgcaggcat ctatcccggc atcgactatc
     8821 tgtgctcatt aaacaccgaa aagaagatca ttacccctgc tcccggctcc aaagcagcaa
     8881 agtattacgc aggctggctg gagactttgt cagccggccg gtgccgccag tagttggcga
     8941 gtatcgatcc cgagatattc atccagggac taaagaccgc agccgcaaga cctaccgtcc
     9001 cgagcttgcc catactgccg gccagcccgg aggccatacc cccgttttgc agcccgacct
     9061 cgaacgccac cgtacgcgct gcattcttat ccagcctgaa cagcctgctt aaccagtagc
     9121 cgaagaaata tcccgccgcg ttgtgaaaca cggaagcaag gaataacaac aaccccacat
     9181 gcagaagatt gtcccgaccc gccgcggtcg tcaccgtcgt aaaatagacg atcccggcca
     9241 tagagatata cggcatcacc cgctgcactg caggccatcc ccgcaccagg tagtgatacc
     9301 cgatgcccgc caggattgcc ccggcaaaaa atccggttat tcctccatcg atccgaccaa
     9361 tccccaatac aaccaaccaa accgcggcga ccgcacccgt ggccaatgtc catcgtctcc
     9421 cctttacaga cgccccgtta agataatcgt gcagcaacgc cgcgccgata ggcaccagca
     9481 cgatcttcac gatctccagc atcatatccc cgaattgcac atggatatac gttcctgcca
     9541 gcagcttcat cagcagtggc gtaaagaatg gagcggccag cgtagccatc gccgtaacgg
     9601 cgacgctcag tgtcaggttt gcccgtgcga tgtataccat cacgtttgaa gcaagtccgc
     9661 tcgaacacga gccgataagg atgatacccg ccgcgatctc cgggtcgaaa tgaaagacac
     9721 gggtgagcag atatccggta agcggcatca cggagaagtg gcatgcgagc ccgataagca
     9781 cacctttccc ggtaccggct aatcccgtga aatcccgcag ccgcatctgc gtccccatac
     9841 cgaacatcac cagctgaacg actatcagca ccagccattt atttcgcaga tcgagggaac
     9901 cccaatggga gaaggcggcg ggatacaaca tccctgccac cacagccgtt acgatccacg
     9961 ccgtatattg atatccggca agccgcggga ccgcacccat gcctatggcc agacacacgg
    10021 agagcgcaag cgcggcgggc tgccagagcc caccgttcga cagacaaaga ccggcaatca
    10081 gcaccggtaa agcgatgccg ctacaccata aacagatcat tctaaacctc agcatcggca
    10141 ggctataaaa cagatttcag gtattcgatc gccgtgggcg gactcgccag gataggaatg
    10201 cgcttgtcag cttcaggcaa cgcatctacc acccgcgcca tcgaagcctg cgcaaggacg
    10261 atcacatcca cctgcgcaga taattctctt agagcgtctg ccaccatctt atcgtgtttc
    10321 gcgggatctc ctcccatcaa cgcatcgaac gcgccttcgc ataacctcga tgtcagctcg
    10381 atggccttac cggctatcgc cgcccttcgt ttaacgagat cgctcgtcgg tgcaagcgtc
    10441 gtcggcaggg tggcgatcac tccgaccctt gtgcctttgg cgaccgcgag atcggccatt
    10501 ggctggtcca cgcgcaatac cggcacgcgt gtcagtgcgg ccgaagcctc taccgccgcg
    10561 cctatcgaag aacaggtgac catgatataa tccgcaccgg cgttttctgc cgagcctaca
    10621 tagtcgacga cccgccgtgc ggtctggctc gtcagctgtc ccttgcggat cacgtcggcg
    10681 atcaggctgt catcgacgat gttaaaaact ttcacccccg gcagcccctt gctggtcagc
    10741 tgctggaaca ccggcaccag tgtcgcggag gtatggatca atcccaatgt cttttgcata
    10801 tcgctatttt tttccattta atagttttcc aaaataatcc tcacccccca cctggccgcc
    10861 cttgagattt atctccatcc catccaccgt cgaacccggc gcataggccc ggcaaagcgg
    10921 cgcaccgggt atcagcggac agatcatctc caccgccccg atccccaacg ctcgcgcggc
    10981 atagcccgat gtgtcgcccc cggcgatcac cagccgcctt actccgtccc accctgccag
    11041 gatttctttc accaattttc ctaacgccgt cccaagcaac gctgccggta tcggacccgc
    11101 cggcgtatgc ccggtatgca ggatcaggct acggccctcc ttcaaatatt cccgtgcttc
    11161 tttcacatga ctgccccact ccgctaccgc ggcggcgtcc agtgcaatcc cggcaaatcc
    11221 attcgccttg gcccacgcga tctgtcctgc cgtcaccggc gaacaggacc ccgagaccac
    11281 cagcaacggc tccgcccggc ccggagacgg ccactccgca ggcggggtca accggccatc
    11341 cgccttccaa tgatcgccta aggccatctc taccgccgat gaaccaacgg agaacagcgg
    11401 ttgcccgggt gatccatatc cgtcgagcaa cgcaccgata cgctcaagct gcgtggcata
    11461 taagacatcg atcaacgtta ccgctgaacg ctccaatatc ttgcgttgct cgttgggtcc
    11521 catttccaac cccacaatgt ccagtagacc gatattccgc gaagtctgcc ggcccagatg
    11581 cacgcgcaga tcactttcat ccgcgggcgt caccggatgt ttgctcatcg acggatgccg
    11641 gtccagccgg tagatgttcc cctcactgcc gatacccatc cgcgcaaaga gattgccgaa
    11701 aacgcaatag cggcccagcg cgggcgcccc tcctaacacc ggaacaaaac ccgcgcgaaa
    11761 aatatctgcg ccgatatcga tcgccttccc gatagaaccg attgccggcg aagagtcgaa
    11821 tgtcgagcac accttataat gcacatgcgg cacacccagc cccgccagca ccccgaaagc
    11881 cggtcgcagc actgcttcca tctcatcggg cggcatagcg cgcgtcatac cggcaatgcc
    11941 tatcgcctcc agatctggat accgtttcag ttgctccgcg ccgggtggct ccatgaataa
    12001 tacggtccgc gccccggccc ggctcaggaa ttccagggca tcggtagagc ccgtgaaatc
    12061 atcgccataa aaagccagca gcaaactcat ttgcgtccga atttttgtac tgatttagca
    12121 aaagcgggat atttcctcgc gccctcctcg accgagagcc cttcgaccgc ggcctgccag
    12181 gcctgccgta aggcggtgac accacccgcg ggtccatccg gatgcgccat aatgccgcca
    12241 cccgccatat acaaaaggtc gatcgtcttc gtccggcggt aggtctccgg cgcctgtccg
    12301 ccccattgac ccgaagagac cacgggaagg atggaatagc ctccgagaaa gggcgtcaga
    12361 caggcatcga tcgatcgtac taccgagtcg tcggactccc agaatttatt ttgtatcccg
    12421 ttgacatgta tctggtctac tcccgccagc ctccataatt tttgatacgc cgggaattcg
    12481 atgcccagta acggatgccg gttgagcata ccccagccgt tgcgatgccc atggatagcc
    12541 agctgccgca tgtcgcacag cttttttacg ccggaaagcc ccacgctgtt cagactgacc
    12601 atggcgcagg tggcgccggc ctttacaagg gtctcatacc gtcgcagcat cgtatcgatc
    12661 tctgcgctca ggttaaaagc atacatcact tttttaccgg tcttatcggc atgttcatcg
    12721 accaccttca tcaccgccgc gacccggtct tcaaaagagg aattggcagc cgcagaaagc
    12781 agttcgtcgt ccttgataaa atcgatcccc gcccccgcca gcgtcttcac gagatccgcc
    12841 gtctgttccg gcgtcatccc gatgctgggc tttataatgg tcccgatcag cggccggtcg
    12901 tatacccccg tcagtcgccg gcacccttcg atcccgaaga caggtccccc gaaatgctgt
    12961 gcaaacgacg gaggaatgtc aaggtccatc agcttcagac ccgtaaatgc acttagctca
    13021 taaagatttc cctgtaccgt ggagatcagt acaggcagat tataaccgca attttctatc
    13081 gaccacgaca cgcggatggc agccctgtgg aagggtccct tctgaaaccc cgcgccaccc
    13141 gggatagccg gatgttccac tgtctccagt tgttccaccg actctacctg cgccgcaaac
    13201 cgcgtcttca gttcttccgt ttcgccgggg acggcgacaa aagtccccga ggattgctct
    13261 ccggctaaaa tggcggcggc ggcggcggga cgtagcggcg tttcgatgtg gtacagggca
    13321 gtgattcttt ccattacaaa cgcaaatgac aggtgttgct gcaaggtaaa aataaatgtc
    13381 aaatgaacgg taattcgcca ggatttcctt ttttcaaata ttttacatac atttagtgaa
    13441 taaatccctt tttatgaaag gaactcacct gggagagttc gaagaactgg tgctgctgac
    13501 cattgcttca ctggtgaaca gcgcctacag cgtagctatc tgtgacgagc tttcgaaaca
    13561 tgccgatcgc atcgttaagc tcggcgtcgt acacgcagtg ctccaccgcc tggaagaaaa
    13621 agccctgata aaaagtgagc ttggcgaagc ctcggcgacc cgcgggggca agcgcaaacg
    13681 cttttataca ctcaccaccg ccggaaaagc atcgctgcta cgctcaaaag agatccgcga
    13741 cacgctatgg caaaagatcc cgaatctgac cttaaaccgc atctgatgaa agaaaaaatg
    13801 ccggaaccgc caaaatgggc cacccgcttt ctcacctggt actgccgccc caccctgctg
    13861 gaagacctgc aaggcgatct caacgagtac tttcaccgca acgtcaggga gcggggagca
    13921 tcctccgccc ggctgatcta tatcatcgat gtgttgaaat tttttcggcc ttatatcatt
    13981 cgcaaacctt ctttcagcaa tcctttaaaa ggaaaaatca cgatgaagaa acaattgctt
    14041 ttcaccctgc gccgtctcag ccggcataag ctgaccgccg gcatcaacat cctgggactc
    14101 acgcttggga tactctcctg tctggtgata ttcctttatg tgtccttcga attcagctat
    14161 gataagttcc acgccgacgc cggccggatc taccgggtca tcgtatcggc ggcgaaaaag
    14221 aacggctcgc atggcagcgc ggacatgaca tcgccacttg gccccgatct ccgccgggaa
    14281 acttccgctt tcagcgccgt caccagcctg cataccgacg acacccgtgt actggtaccg
    14341 ataacaggca agccggcgcg ggtgatccct acgatcggcg atgacgaacg gcagcacatc
    14401 acgtttgccg attccgacta tctgaagata tttcattatc ggtggctggc gggaaatccg
    14461 gcgacggccc tgcagaaacc tttttctgta gtactcacgg aatcggaggc gcagcgctat
    14521 ttccagggcg gccagccgga agactggatg ggccgttcca tcgtgtatta tgattcgctg
    14581 ccggttgccg tgacaggcat cgtcaaagac tgggaccaca acagcgatct tggcttcagg
    14641 gacctgatct cctacaatac cattgaacac agttttctgc aaaaggacat ggagggctgg
    14701 aatctttggg caggcaacgt taatgtctat gttaagctgg cgccgggtac tacacccgca
    14761 caggctgaac aacaattgcc ctccttttta caacggcaca ttcaggccaa tgggaaagcc
    14821 gtcctgtcct tacagccgct ggcggacatc catttcaatg gggaatacgc cgaccggtat
    14881 ggccgccggg cgcataagcc tacgctatat gcgctgaccg gtattgcgct tttcatcctg
    14941 gtgatcgccg ccatcaattt tatcaacctc tcgacggcgc agtccatact gcgggcaaga
    15001 gaggtaggcg taaggaaagt gctcggcagc agccgcggca gcctcgtccg gcaattcctc
    15061 aacgagaccg ggatcatcgt gacgtcggcc atggtactcg ccctcctgct ggccaatccc
    15121 acgatcaggg ccttacacgg ctttattccc gaaggggtcc ggctgcatat cggcgatgtc
    15181 tatacgtgga tttttgcagg atcgacgatc ctggtcacct gtctgctcgc cggctggtat
    15241 ccggcccggg ccatctcctc tttcctgccc gtgatcaccc tccgcggcca gggcgtccaa
    15301 cagctcaact ccaaaagcta tctcaggaag agccttatcg ttttccagtt taccgttagc
    15361 ctgatcttta tcatcggtac catgatggtt ggccggcaga tccgttacat gctgaacacc
    15421 gatcttgggt ttaaaaagga cgccattgtt acgatcggcc ttccatggga tcagcctaag
    15481 gatcggaaga tcgtactcgc caccgggatc cgcagattag ccggcgtgca ggaggtcagc
    15541 gtaaacacgg gcagtccgga ggccgccagc catggcggaa ccggtatcga atataacgga
    15601 aagacacaca cgaagatcga cgccggcttc gttcagatag accccgacta tataccccta
    15661 tacgagctgg cgcttgtggc cggcaggaat ttcactatcg cggacacggc gcgacgcggc
    15721 agcgatccgg cttcggggga ccccgggccg gcgcaaaccc ccttccgtgc tttcatcctc
    15781 aacgagacgg ccgcaagggc cctgggtttc aacaaccccg ccgacgcggt aggccaacag
    15841 gtcagtagcg gtttcagtgg gataccggga cccgtcgtcg gagtcgtcag ggatttccat
    15901 tcgcagaatc ttcatgagaa gatacagccc ttcttcttta cggagcagcg tgccgcaggc
    15961 ggtaccctta gcataaaact ttccagcgcc acccttccgg ccgttcaggt gaagacgttg
    16021 atgacggagc tggaaacgag ttttaaaaaa atatacccgg catcggtgtt ccaggcccgg
    16081 ttctttgatg aatcgctggc gcggctttat cgtcaggaac aacaaaccgc ggagatcatg
    16141 aatatcgcca tgggcatcgc gatctttatc tcgtgtatgg gactcttcgg tctggcagcg
    16201 ttcaccgcca accaacgcac acgcgagatc ggcatccgta aagtgctcgg tgcaaacgtc
    16261 ccccaactgg tatcgatgct ttcgcggcaa ttcatcctgc tggtcatgct atcgacgctg
    16321 atagccgcac ctatcgcagg ctggggcatg catatgtggc tggaggactt cgcgtatcaa
    16381 acctctatgc cgtggtggat ctatgtggtg gccggcgcag tggccgtagc gatcgcactg
    16441 gtaacggtca gtttccaggc catccgggcc gccatggcaa acccggtgag gtcgctgcgg
    16501 agcgagtagc aaatcttgcc ggaggccacg ccgtcagagc aaaataatgc agggatcagg
    16561 gcttcggacc gcgttctttc gcctccttga tgttcagcgg attggcgccc agcacctttc
    16621 cctgcaacgc ctccatggcc tttcgcgctt cctcgtcccg cttcatctca acgatgccct
    16681 ttcctctgct aagccctgta ataggatcga tcagtatctt gctataatcc acccaaccga
    16741 atggattgaa cagacgctgc agatcaaact tgtcataatg ccggggaagc ccggagattg
    16801 tcattcgcat aaaacaaaat aataaaaaac cgtcaaaggt cggtcttttt gcgcttttcg
    16861 cgcgcgcctt ttggccaccg acccacaaat tttctggtcc cccccgccgc ttccggcata
    16921 ctcaatcatg cccagataaa tgcataatta actcataatc aatcaactaa taacccttgt
    16981 cggtgcttcg catggcccgt tccctctttc gaaaacggca ggaggcactc accaaatttc
    17041 gtgggtctcc ttcacctccc gctctgcggg gggatttcta atcttctcgc tgttaaacca
    17101 acttcctttt ccaaaacgat tttttcatct tcatatcaca tgtgtatttc agaagcgcaa
    17161 ttatcatatt ggcattgaaa tagataagct taccaacagt atagtaaata ccatttccgg
    17221 agacagcttc gacaccgaaa ttttgcccgc aacaaaggat gatctcaaag gaataactaa
    17281 aaagataggg tggaaattct tgtagggtgt cggtggaaag gggatatgaa ggtttcgtgg
    17341 ctttcatttc caagacgaaa ttgataaggc attacgaaga aaccccggga gcccactgca
    17401 ttggaggtca gcgcatggca ataggccagg aagccgctgc caaacttatt gaccgttact
    17461 ttaaattcaa ataaatggga catattaaag agcctaaagg tgtagatttc gtggtcgaac
    17521 cgcatgttgt aacggctgaa gacaggcgaa ttatcagcga ggccattgcc cactataagg
    17581 ccaccggaaa gtttacgggg acgcccaaac ctaagagggc ccgccgtaca acaaagaaga
    17641 aggctaagaa atagtccggg aataaacttt ccgcaattta ccaattcaat catccccgcc
    17701 tcacttgagc agaccaaaaa atttagctta ttctattctg aaacgaacga ggtcatcacc
    17761 accacattca gcccgcactg gtcttcccac aaataaaaaa agcggacaaa atggactctt
    17821 ccatcttatc cgccagggag gttccgagca gattcgaact gctgtagaag cttttgcaga
    17881 gctctgccta gccactcggc cacggaacca atcaaacccg ccctttcagg cggcaaacaa
    17941 ccaaatcacc tccttcgagc gatttttttg agtggctgca aaattaagat attttcgcag
    18001 aagtttaaaa aatattatgc agccaatccc tgacagcgag ctcattatca atagccgcgg
    18061 tgcactatat cacctcgatc tccggccgga agaactggcc cccatcgtcc tcaccgtcgg
    18121 cgatcccgga cgggtcgaaa aggtaagccg gtacttcgac tctgtcgaac atcgcagcag
    18181 ccaccgcgaa tttatctcgc ataccggccg catcgggcag aaacgtctca cggtcgtctc
    18241 caccggcatc ggcacggata acatcgacat cgtactgaac gagctggacg ccctcgtcaa
    18301 tatcgatctc gacacccgga tggtgaaacg ccaattcacc agtctcacca ttatccggat
    18361 gggcaccgcc ggggccttac aaaaagatat ccccgcagac gaattcgtag cctccaccca
    18421 cggtctcggc atcgacaacc tgctcaactt ctatcgccat gacatcagcg aagaagagaa
    18481 cgagctcctg cattcctttt ccacgcaaac acagctgcat caccggctgg cccaacccta
    18541 tatcagcagc gccagcggct cgctgctccg ccattttacc accggcttcc atcacgggat
    18601 cacggtcacc tgccccggtt tctatggtcc gcagggacgc gtgctgcggc ttgggctcac
    18661 ccaacccgaa ctgatcgacc gtctcaccgg gttcacctat ggtcctcacc gtatcgccaa
    18721 tttcgagatg gaaacggccg ggatctacgg tctcggcaaa ttgctggggc actcctgtct
    18781 gagtcttagc gccatcgtcg ctaaccgcgt cctgaagaca ttttcaaggg acggcgatgc
    18841 tacggtggac cgtctcatcc gcgggaccct gcggatcgtc gaaggactct gactaggccg
    18901 gttcgcccga atcaactatt tttgtccatc gttatgagtc aactgcattt tttcaaatac
    18961 cagggcaccg gcaacgattt cgtcatcctg gataaccgca attggagcta tactgcgcta
    19021 actacggaac gtatccgctt tctatgcgat cgccgttttg gcatcggcgc cgatggcctg
    19081 atgctgctca acccccactc cggctatgac ttcgagatga aatactacaa cgcagacggc
    19141 cgcgagagta cgatgtgcgg caacggagga cgttgtctcg tcaagttcgc ttacgatctg
    19201 ggcatccgca agaatgaata tagttt
//
LOCUS       DASYSV010000502         2927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1386991, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000502 DASYSV010000000
VERSION     DASYSV010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386991"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /gene="bioA"
                     /locus_tag="VGQ51_15550"
     CDS             <1..1270
                     /gene="bioA"
                     /locus_tag="VGQ51_15550"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEV9038049.1"
                     /translation="PYTQHKGWLPPIPILKGKNTWLFDDQGKGYIDAISSWWVNIHGH
                     AHPHIAEYIYQQALRLEHVIFTGFTHEPAVTLAERVLPLLPGDFSRVFYSDNGSTAVE
                     VAIKMAIQYWSNQGLSRRKILAFHHSYHGDTFGAMSVSERSLFTDAFRDYLFDVVFIE
                     TPTPQNLDRLLSFIRREGNSIAGFIYEPLLQGAGGMRVYDAGSLDVLLQATSHEGILS
                     IADEVLTGFGRTGRLFAGEYLRNRADIICLSKGLTGGTMALGVTATTEAIFEAFLSDD
                     KRKTLFHGHSFTANPLACAASLASLDLLLQEDCLSRIRRIDDRHTGFLETLGEFSNVR
                     NPRTLGTILAFELDAGEDSYINAIGPAITNAALEAGIYLRPLGNTVYFMPPYCITENE
                     MDQVYDFLVQCLVRYSVAKPAEPARPPGNH"
     gene            complement(1280..1786)
                     /locus_tag="VGQ51_15555"
     CDS             complement(1280..1786)
                     /locus_tag="VGQ51_15555"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEV9038050.1"
                     /translation="MRVTHAILTFFAIALFGTAGYAQPNHPHSPHDTIHAKDITITYG
                     RPYKKGRDIFGGTVAPYGKTYRCGADEPTIVTFDKDVTFAGKPVKAGKYSFFAVPEKD
                     KWTVILNSNTTMWGTQHDQHADQDVLKVEVPVHNLSTPVEQLTMKAGAHEISLEWDKV
                     KIEVPIKM"
     gene            complement(1832..2338)
                     /locus_tag="VGQ51_15560"
     CDS             complement(1832..2338)
                     /locus_tag="VGQ51_15560"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV9038051.1"
                     /translation="MKFLIISLISVFVVLTALSLLFPSDLRVQRVVNIASPRGKVLAA
                     VGDLKTWPEWNRFVRGPGLTHMSISDPSSGIGARLSSDQMTISETAADSDGITLDWDL
                     KGGKRHSGGISVGMLNRDSITVEWWFDLHFRWYPWEKLGVFVYDRKLGPIMEQSLDSL
                     RRYVENSR"
     gene            complement(2363..>2927)
                     /locus_tag="VGQ51_15565"
     CDS             complement(2363..>2927)
                     /locus_tag="VGQ51_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038052.1"
                     /translation="SLLSAAVFGVAFLFFSLRSQLVLKRYYATPIGKRYIVLGEGLAR
                     VVFQLITAVVIIGIGKIGFHFTLVHGWLTFFELLVLSLIGLIVFMGFGFIISSVARSE
                     STIPPFANVFTLPQFLLGGTFFSTDVFPKWLQRICEILPLKQLNDALRNVAFEGAHLT
                     DCWKQLGILAIWGILTYAVAVRVFKWE"
BASE COUNT          674 a          874 c          744 g          635 t
ORIGIN      
        1 tccctacacc cagcacaaag gctggcttcc ccccatcccc atccttaaag gtaagaatac
       61 ctggttattc gatgaccagg gtaaaggcta tatcgatgcg atcagcagct ggtgggtcaa
      121 tatccatggt catgcccatc cccatatagc cgaatacatc tatcaacagg ccctgcgtct
      181 cgagcacgtc attttcaccg gatttaccca cgagcccgcc gtgacgctgg ccgaacgggt
      241 gcttccattg ctgcccgggg attttagccg ggtcttttat tccgacaatg gctccacggc
      301 ggtagaggtc gccataaaga tggccataca atactggagt aatcagggtc ttagccgccg
      361 caagatcctt gccttccacc actcctacca cggcgatacg ttcggtgcga tgagcgtcag
      421 cgaacgcagc ctatttacgg acgcgttccg ggattacctg ttcgacgtag tcttcatcga
      481 aactccgacg ccgcaaaacc tggaccgtct gctgtccttc atccgccggg aaggcaatag
      541 catcgccggc ttcatttatg aacccttatt gcaaggggcg ggcgggatgc gggtctacga
      601 cgccggctca ctcgacgtcc tattacaggc cacttcccat gaaggcatcc tgtcgattgc
      661 cgatgaggtg ctcacaggtt tcggccggac aggcagactc tttgcaggcg aatatctccg
      721 caaccgggcc gacatcattt gtctcagcaa aggtctcacg ggtgggacga tggccctggg
      781 cgtcaccgcc acgacggaag ccatcttcga agccttcctc agcgacgaca aacgaaagac
      841 acttttccac ggccattcct tcaccgccaa cccgctggcc tgcgcagcgt cactggccag
      901 cctcgacctg ttgctgcaag aagactgtct gagccgcatc cgccggattg acgaccggca
      961 taccggattc ctggaaacac tgggcgaatt ctccaatgtc cgcaatcccc ggaccctcgg
     1021 cactatcctg gccttcgagc tcgacgcggg ggaggacagc tatatcaatg ccatcggacc
     1081 agccatcacc aatgcggcgc tggaggccgg catctatctc cggccgctgg gcaataccgt
     1141 ctatttcatg ccgccctact gcattaccga aaatgaaatg gaccaggtgt atgatttcct
     1201 ggtccaatgt ttagttagat attccgttgc gaaaccggcg gagccggctc gcccaccggg
     1261 caatcattag cgccgcccgt tacatcttga tgggaacctc gatctttact ttgtcccatt
     1321 cgaggctaat ttcatgagcg cccgccttca tcgtcaactg ctctaccggc gtcgataggt
     1381 tgtgcaccgg cacttccact ttcaggacgt cctgatcggc atgttggtca tgctgcgtgc
     1441 cccacatggt cgtattggag ttgagtatca ccgtccactt gtctttttcg ggcacggcga
     1501 agaaggaata tttcccggct ttgaccggct tgcctgcgaa ggtcacgtcc ttgtcgaagg
     1561 tgacgatcgt cggctcatcg gctccgcaac gataggtctt gccatacggc gcgaccgtac
     1621 ctccgaagat atcccttccc ttcttataag ggcggccata cgtgatcgtg atgtctttcg
     1681 cgtggatcgt gtcatgcgga ctgtgcgggt gattcggctg ggcgtaaccg gcagttccga
     1741 acagcgcgat ggcaaaaaag gttagaatag cgtgtgttac tcgcatattg gtggttttta
     1801 tgtgacgaaa atcagttgcg ggagactaaa gttaacggga attttccaca tatcgccgaa
     1861 gactatccaa agattgctcc atgatgggtc ccagcttccg gtcataaacg aagacaccca
     1921 gcttttccca cggataccag cgaaaatgca gatcgaacca ccactctacg gtaatgctgt
     1981 cgcggttcaa catacccacc gatatcccgc ccgaatgcct tttgccgccc ttcagatccc
     2041 aatcgagggt gatcccgtcg ctgtcggccg cggtctcgct aatggtcatc tggtccgacg
     2101 acaacctcgc ccctatgccc gatgaaggat cggatatgct catatgggtc aacccgggac
     2161 cacggacaaa tcggttccac tccggccagg tcttcagatc gccgaccgcc gcaagcactt
     2221 ttccacgggg actcgcaatg ttgacgactc gttgaacccg gagatccgat gggaacagca
     2281 gcgacaacgc cgtcaacacc acgaaaacgc tgatcaggct aatgattagg aatttcacat
     2341 attgcatact cgagacctcg ttttactccc atttaaagac ccgcacggcg acggcatagg
     2401 tcaatatccc ccagatagca agaatgccca gctgtttcca gcaatccgtc agatgcgccc
     2461 cttcgaaggc tacgttgcgc agggcatcat tgagttgttt caacggaagg atctcgcaga
     2521 tgcgttgcag ccatttggga aatacgtcgg tagaaaagaa agtgccaccg agcaggaatt
     2581 gcggaagcgt gaacacattc gcaaaaggcg gtatcgtgct ctcgctgcgc gccacgctgc
     2641 tgatgatgaa tccaaaaccc atgaagacga tgagacctat caggctcagc accaatagct
     2701 cgaaaaaggt cagccagcca tgcacaagcg tgaaatggaa accgatcttc ccgatgccga
     2761 tgatcaccac ggcggtgatc agctgaaaga ccacccgcgc cagtccttca cccaatacaa
     2821 tgtatctttt gcctatcggg gtcgcataat accgcttcaa caccagttgc gaccggagac
     2881 tgaaaaataa gaaggccact ccgaaaaccg cggcactcag cagcgag
//
LOCUS       DASYSV010000503        11136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1511704, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000503 DASYSV010000000
VERSION     DASYSV010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511704"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(490..1386)
                     /locus_tag="VGQ51_15570"
     CDS             complement(490..1386)
                     /locus_tag="VGQ51_15570"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV9038053.1"
                     /translation="MKVYALRSVPVRSLLQWAGLAPSSFYYKSSGARKGIKPSTHSLT
                     IHGERMENKVVVVQMEVVLRQEFCCYGYKNMTAELRELGWIINHKKVYRLMKEARLLY
                     GARIRPLPFKRNFIRFRSLKPERPLQYLAMDIKYVYIHGTGRNVLLLTVIDVYSRKVL
                     IYQLRSHIRKEDVLVMLSLMLLEYKAEGMSIRNDNGSQFIAQVVRAFLKEKGILQEFS
                     HVGTPEDNAYIEAFHSNLQREVIDRYEFDSIYHAQMVLDRYYEWYNEKRRHGALNRQT
                     PNHMFQNINPVPFENEKLLNYL"
     gene            complement(1383..1703)
                     /locus_tag="VGQ51_15575"
     CDS             complement(1383..1703)
                     /locus_tag="VGQ51_15575"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV9038054.1"
                     /translation="MSKQRRKFSVEQKLQIIQEADQHGITQTLRKHNVSHSVYLRWKQ
                     QFDKGGISSLAPQYRKVDPELRALQEENARLKKIIGNQALELEFKTELLKKNEAYERR
                     LKGS"
     gene            complement(1738..2247)
                     /locus_tag="VGQ51_15580"
     CDS             complement(1738..2247)
                     /locus_tag="VGQ51_15580"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9038055.1"
                     /translation="MNPKPSSMQAIIGRKEEQIILNQLLESKRPEFLAVYGRRRVGKT
                     FLIRQAYASRIVFQMTGIARANTSQQLTNFFSALRNMNKNTDQDTLPKNWFEAFEMLK
                     SCFGKSSEPKKVIFFDEMPWIDTPRSNFLSALEHFWNSWASDRPDIILVVCGSAASWC
                     TDPLNGWTN"
     gene            2596..5343
                     /locus_tag="VGQ51_15585"
     CDS             2596..5343
                     /locus_tag="VGQ51_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEV9038056.1"
                     /translation="MENTFRYLVVEDDEFDRVSLDAEAGKFPFLKKIGACDHPLQAME
                     LISRHQPDLLFVDIEMPGMSGIELLRLLKGQPFLTVFITSHPEFAVEGFELNAFDYLV
                     KPLTAERFERCALRLRDFGELRHKAYAYEKEQESGVIVIKQGHDKCKVKLTDIQYLEA
                     MKDYTRLVTIDKQYLVLTTLSDLLGKLPKEKFVRIHRSYAVHTEKVTGVGKNKVELAA
                     AELPVGKLYRHAIKGVLTLLILFLALWGMGQGYKEKMDDLAAKCDSLRPSASTDHAKL
                     RTAGLEGIRIAHADDYFHQSQFAFFAAVGYYYETRFDSAQYYFYKSLENGKKGHLTRQ
                     IELACITLIPVNFQLQQVSKMDSCKNILQSIMDTTHDRQILEDGYYSLGQYFQNKSYY
                     STSQDYFIRSIELREKDQDIATDSKKEFDFAIQCDMLSKLYLVTQMTDKSLGALRRGQ
                     RFASVAPLVRNRLASSFVEAFTESGQIDSALYYDRQLEANAVNPIPYSSELVSSDLDI
                     AGFYLDHGDADAALRYIVKADTIAAKIESPILNFQTEFTRARYLIAKKQYPPAIAQLE
                     LSIPVAQQVAKDQYAGDLKYMAEAQEGKGDLAAALHYYKEYVELTDSLNKDKLSQTFA
                     DLETHYQTHEKELQIESLNKENQVHLLELKNARQTRLILVLGLGGLGVISLLLYFFYR
                     NKARLNRELARANDTKATLFGIIGHDLRAPVGKIVRMLQLQNQRPELFTPEQRKTHEE
                     SVKKASENVLETMEDLLVWSKSQMEHFHPEYHRVVIRDVLDKEIGSMQDQLEEKQVRI
                     VDEVPAGLSRESDENFLSVIVRNLLQNAVRHSDGDRRIVVAATPTEVTITNPTSMGDA
                     RALNRRISQGMIGSGTSGLGLQLASDLAGRIGAKLYFRGEDGVSLTAVLSWDGASGYI
                     A"
     gene            5533..6678
                     /locus_tag="VGQ51_15590"
     CDS             5533..6678
                     /locus_tag="VGQ51_15590"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HEV9038057.1"
                     /translation="MRQTTLFMIALALLAIAQPLCGQAQFLKTLTNNLKQTLQNRANG
                     KSNQTTNVILDKIDSATRVGGTKTGKTTATGAPGTQGMTGTTNAGGQTGGLPNVFGGS
                     VDTSGLSRVLGAFAKTAQENPNDTSQADLVMKSLGRLTGGDGVSPQDSAAAIKSFMTA
                     SGGSGVLYQTITTTTSKRGNTRDTSSLWMTNSGEGRSEMRIPIPGAVTPKFVVIGRAN
                     QPTYSIILDAANRSYSLNVIDTALINSGIEKYQVTRVGTETVAGYSCIHTKIVQTTGS
                     GMFKSTTAMELWTSTTVPGYAIYSKLISFQSSNGGFLGALNKAGAGGFLVKMTTGDGK
                     DVSMTMQLWKAEQKSFPASLFAIPAGYTNDEKTTAQRMLSGSAPAKH"
     gene            6692..8401
                     /locus_tag="VGQ51_15595"
     CDS             6692..8401
                     /locus_tag="VGQ51_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038058.1"
                     /translation="MTAKLAIFLLLFLPATYTRAQNLVDLRDQKPFTLHGSLGAGMEF
                     YKSNEPYPTRPPFAWNIYGSLMPSVYGISLPMSFVVTQYGNSYSNPFTQIGISPSYKW
                     IRLHLGYRTIRFSPLVFAGQSFLGGGVELSPKGFYFGAFYGLLNKAVQEDTSFAHPIE
                     PQYARMGYGFKIGVGSPKNNFGFSFFHAKDDSGSIRRMPDSLTTILPQENTVVGSSWH
                     FTFFHRLTWTGDLAAGLLNRDQSYQEIDSVGYYKIPTLMRKIMPVNWSSVFSYAGQSQ
                     LSFTWKTVNASAGYRRIAPDFVSLGVPYMLNDLEMMNGSFGGNFDKGRLNANLAITTQ
                     HNNLSHLLASTLLTRTGNLSLNAFVSQHFNINATVTGVQVYQRDGAVALSDSVRMNQL
                     MISFTLAPTLNFTGNDYQHTVNANISYTDLDDHNPATAAMTGGNNISTSLNYALYFSH
                     AYWGLNAAVLYSQYGQGDNRYQSTGINAGLNAQLLSSHQLALQGSAGYFLNKYSNSPT
                     GNNTTFAFSGNYTMALHTLGVYLNYVLTPPVNLNPLTTIYHIPIAVNSKNFAGGVTYG
                     FHF"
     gene            8411..>11136
                     /locus_tag="VGQ51_15600"
     CDS             8411..>11136
                     /locus_tag="VGQ51_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEV9038059.1"
                     /translation="MIRIFTFCSLLLLSLTGWTQFTVGVQVNVVQPVPPYLPQLKADI
                     MGNRAGQLNQDISSHLSIILRYTGRTAQRVKLAGSIERISPAVMGVSLRPDFQPAQPI
                     VLGPGQPILSLTRDMLQNAFGNFTENSLVYTNCDLNTLRQNGIDFKLPEGTYRVCVTA
                     YDYDRPGFSAPLSAPGTGCAYFTICYTASAPQLLLPVSTFINATGGFQDVTPHSNLVQ
                     FTWTPPSTTCGLPMGPLTYELEIKRVFNGQSVMDAVNNPFVFHQQNIPVTTFLLDTLK
                     YGHILVPGQQYIVRVKANFIPMVGSPLEIANQGWSEIGAFDWAPVSYFPKTAVAVNHP
                     TPDKPVPPGSIQPGGYINEPYTPIAACAVATPVSNTSPLPGSPAGQQVTIDGFLMQIS
                     QATANTDGSYKGSGTISWHPFSGDIRLNVSFDSIRVNTDKVVYAGTAQTVSDNGYPDW
                     SVFGAAGADPVTKLAGLDDGVLSAIKTRIGDGSHLLNTGGGGPVSFPMGLSTTLGGAP
                     ATLAVMGISFRSSCTNMDVLFDLNLPDIGGWLALAGAGLSIGPNSLLAGTGGVLYLPQ
                     DKSFAAGGINYTIDGCPGAGASAVDTSKGTYIAWDAVKGLSAVVANADIRFTDGNAIV
                     AVDKNDQRLTTPATIHARFAFSDWNDWVATAVLPNDFELAGLPGFPIRSDGLFFDHST
                     RQNPGGIGFPTGYTGAEDAGFEGLYIPALSMSLPASFQTFSGGKPGAINFTGFILDDN
                     GVTTNINAANILDINSGSLGGWAFSIDKINIAVVNNNFQSGMAMSGQVRLPVSSDGLS
                     YTCALNSVDGKVDYRFVVQPGGGLNVPLWLAKLNLDPNSSLVISSDPTGMAVKTHLNG
                     NIGITIDHSGMPKLSLPALSFQDMAMANRADTSAGAGAGFFFSPGKWSLGGGV"
BASE COUNT         2583 a         2820 c         3097 g         2636 t
ORIGIN      
        1 ctgcgctctt tcaatgattc aaggactact ttcgttttaa attccgcggt aaatttacgt
       61 ctgctctgct tagtcatggt ggtaatttaa gattttattt ttttatctta actacctgtc
      121 cttttttcgg ggatcaccat atttcaatat gccggtcggc atgaacaaat aacgagcgat
      181 agagattatc gtattcggtc gtcagaaagc cattgggtga aaacgccaat cgatcaatct
      241 cctgaaaagc actcatccct tgtctaattt cgctcaaata aaacggtacg ccaccgagtg
      301 ccatatacaa ttgaagaatt tgatagcggt caagggcgat ctgattggcg cggaggaagg
      361 tttccgtctc gcatagcgtg aatggtaaca gccggattct ttgcgttacc ctattatgga
      421 gccctcccct gttgttgatc actttgttaa tcatccactg tctcgacccc ttataaactg
      481 gacagttttc taaagatagt ttagtagttt ttcattttca aaagggaccg gattaatatt
      541 ttggaacatg tggttcgggg tttgccgatt cagtgcgcca tgcctccttt tttcgttgta
      601 ccattcgtaa taccggtcca ggaccatttg ggcatgatag atcgagtcga actcataacg
      661 gtcgatcacc tcccgttgga ggttggagtg gaaagcttcg atgtaagcgt tgtcttccgg
      721 ggtacccacg tgggagaact cctgaagaat gcccttctcc ttcaaaaagg ccctgaccac
      781 ctgggcgatg aactgggagc cgttgtcatt gcggatagac atgccctcgg ctttatactc
      841 cagtagcatc agcgatagca tcaccaggac atcctctttt cggatatggc ttcgcagctg
      901 gtagatcaag acctttctgc tgtagacgtc tatcactgtt agcaggagca catttctgcc
      961 tgtcccatgg atgtacacgt atttgatgtc catcgccagg tactgcagag gtctttccgg
     1021 cttcaggctc cggaaccgga taaagttccg cttgaacggc aggggcctga ttcgcgcccc
     1081 atatagcaat cttgcttctt tcatcagccg atacactttt ttgtgattga tgatccatcc
     1141 cagctcccgg agctcggccg tcatgttctt atacccgtag cagcagaatt cctgtcggag
     1201 aaccacttcc atctgaacaa caacaacctt gttttccatt ctttcgccgt gaatggtgag
     1261 gctgtgcgtg ctgggtttaa tgcctttgcg agcgccagag ctcttgtaat aaaagctgct
     1321 gggtgccagc ccagcccact gcaacaggct tcgaaccggg acactgcgaa gcgcgtacac
     1381 cttcacgagc ccttgagcct ccgctcgtag gcctcgtttt tttttaatag ctccgtcttg
     1441 aactccaact ccagcgcctg attgccgata atctttttca gccgggcatt ttcctcttgt
     1501 aacgccctga gctccggatc caccttgcgg tactggggag ccagcgagga tatgccaccc
     1561 ttgtcaaatt gctgcttcca ccgaagatag acggaatggg aaacgttgtg ctttcggagt
     1621 gtctgagtta tgccgtgctg atcggcctcc tggatgatct gaagcttctg ttctacagaa
     1681 aacttgcggc gttgctttga cataataccc taaatttaaa tgactaaatt gatttgatca
     1741 attagtccag ccatttaggg ggtcagtaca ccacgatgcc gcagagccgc aaactaccag
     1801 gatgatatct ggtcgatcgg atgcccagga attccagaaa tgctcaagtg ctgatagaaa
     1861 atttgaacgt ggtgtgtcga tccatggcat ttcatcaaag aagattactt tcttgggctc
     1921 gcttgatttt ccaaaacagc tcttcagcat ttcaaacgct tcaaaccaat tcttcggcaa
     1981 ggtatcttga tcggtgtttt tgttcatatt cctcagtgca gagaaaaagt ttgtcaattg
     2041 ttgagaagtg ttggccctgg caatgccagt catttgaaac acaattcgac tcgcataagc
     2101 ctggcggatc agaaaggtct tgccaactct tctccggcca taaactgcca aaaattccgg
     2161 tcgcttgctt tcaagaagtt gatttaaaat aatttgttct tcttttcgac ctattatagc
     2221 ttgcatggat gatggtttgg gattcatcaa agataagatt ctacccactt agcgctaggt
     2281 ataattacat aattggcgac aagtggcata aaacacccaa ttagcgctgg ctatgtgctc
     2341 caggcaggcc atcccttttc ttatggcgcc gcaactcaca ttaggacatt ctatcattcg
     2401 ttcgtccacc taaaatggga aaaccatcga aaacattcgt tttcaaatca ctagataacc
     2461 taagcacttc aatcatagcc ctgctaaaaa ccagtatctt tataaaagcc tgctagtgcg
     2521 tttccctgac ggagagactg acatagtctg cgattgcccc aggcaccccg aataagctta
     2581 ttaatcgacc tctatatgga aaacaccttt aggtacctgg ttgtcgaaga tgacgaattc
     2641 gaccgcgtca gtctggacgc ggaggcggga aagttccctt ttcttaagaa gatcggggct
     2701 tgtgaccatc ctctacaagc tatggaactg atctcgaggc atcagccgga cctgttgttc
     2761 gtcgatatcg aaatgccggg aatgagcggc atcgaattgc tccggctttt aaaaggtcaa
     2821 ccctttctca cggtctttat tacatctcat cccgaatttg cggtggaagg ttttgaactg
     2881 aatgccttcg actacctggt aaagccgctg acggcagaac ggtttgaacg ctgcgcctta
     2941 cggttacgcg atttcgggga gctgcgtcac aaggcgtatg cctatgagaa ggagcaggag
     3001 agcggggtca ttgtcatcaa acaaggtcat gataaatgca aggttaagtt gacggatatc
     3061 cagtacctcg aagcgatgaa ggattatacc cggctggtaa cgatagacaa acaatatctc
     3121 gtgctgacga ccctgagcga tcttctcggg aaattgccga aggaaaagtt tgtccgtatt
     3181 caccgaagtt atgcggtcca caccgaaaag gtcacagggg tcggcaagaa taaggtggaa
     3241 ctggcggcgg ctgagctgcc cgtgggaaaa ttgtaccggc atgccataaa gggagtgctg
     3301 acgctgctga tcttattcct ggcgttgtgg ggcatgggcc aggggtataa ggagaagatg
     3361 gatgacctgg cggcaaagtg tgattcgttg cggcccagcg ccagtaccga tcatgcgaaa
     3421 ctgcgaacgg cgggtctcga aggtatccgg atagcgcatg ccgatgatta ctttcatcaa
     3481 tcccaattcg cctttttcgc ggcggtcggc tactactacg agactcggtt cgacagcgcc
     3541 caatattatt tttataaaag cctcgaaaat ggaaaaaaag ggcatttgac aagacagatt
     3601 gaactggcat gtattacact gataccggta aatttccaat tacagcaggt gtccaaaatg
     3661 gacagctgta agaatatcct ccaatcgatc atggatacga cgcatgatcg ccagatcctg
     3721 gaagacggtt attattcatt gggccagtat tttcagaaca aatcctatta cagtacgtcg
     3781 caggactatt ttatccggag tatcgagctc cgggagaagg accaggatat tgcgaccgat
     3841 tccaagaaag agtttgactt tgcgatccaa tgcgacatgt tgtctaaact ctatctcgtt
     3901 acccaaatga cggacaagag tctgggtgcg ctgcgaaggg gtcagcgatt cgcctcggtc
     3961 gcgccattgg ttcgcaaccg gctcgccagc tcgtttgtgg aggcctttac agagtcgggg
     4021 cagatcgact ccgcgctgta ttatgaccgg caattggagg ccaatgcggt aaatccgata
     4081 ccgtattctt ccgaactggt gtcttccgat ctggatatcg cgggctttta tcttgatcac
     4141 ggggatgcgg atgccgcgct gcgttatatt gtaaaggccg ataccatagc ggcgaagatc
     4201 gaaagcccga tcctgaattt ccagacggaa ttcacacgcg cccgttatct catcgcgaaa
     4261 aagcaatatc cgccggcgat cgcgcaattg gaattgtcga taccggttgc gcaacaggtt
     4321 gcgaaggacc aatatgctgg cgatcttaaa tatatggcgg aggcacagga ggggaagggc
     4381 gacctggctg cggcattgca ctactataaa gaatatgtgg agctgaccga ttcactcaat
     4441 aaagacaaac tctcccagac gttcgcggat ctcgagacac attatcagac ccatgagaaa
     4501 gagctgcaga tcgagtcgct gaacaaggaa aaccaggtcc atttgcttga attgaaaaac
     4561 gcccggcaga cgaggctgat actggtgttg ggtctgggtg gtctgggcgt catttcgctg
     4621 ctgctctatt tcttctaccg gaataaggca agactcaacc gggagctggc gagggcgaac
     4681 gataccaagg cgacactgtt cggtatcatt ggccatgacc tgcgggcgcc cgtgggaaag
     4741 atcgtacgga tgctccaatt gcaaaaccag cggccggagc tgtttacgcc ggaacagaga
     4801 aagacgcacg aggaaagcgt gaagaaggcg tcggaaaatg tgctggagac gatggaggac
     4861 ttgctcgtct ggagcaagag ccagatggaa cattttcatc cggaatatca ccgcgtggtg
     4921 atccgggatg tccttgacaa ggagatcggg tcgatgcagg atcagctgga agagaaacag
     4981 gtccgtatcg tagatgaggt gccggccggg ttgtcgaggg agtcggatga gaatttcctg
     5041 tcggtgatcg tgcgcaacct gttgcagaat gcggtgcggc atagtgacgg agacaggcgg
     5101 atcgttgtcg cggcgacccc aaccgaagtg acgatcacta accctacaag tatgggggat
     5161 gcccgcgcat tgaaccggcg gatcagccag ggcatgatcg gcagcgggac atccggtttg
     5221 ggtttgcagc tggcctctga tctcgcggga cggatcggcg ctaaattata tttccggggg
     5281 gaggacgggg tgtcgctgac ggcggtgttg agctgggatg gggcgagtgg gtatattgcg
     5341 tgaggagaaa gttgtccaac gattttgaaa gcacgttcgt cgagtattcc ggcgaacgtt
     5401 tttttatgtc cgcatttttg ctggtcaacc aaaggatatg ccggctgcaa aacggacaac
     5461 atggatcgaa tggctactgc ttgtgctggc gctccttttt ttcattagtg cactcatcct
     5521 aatcatttga ccatgcgaca gactacgtta tttatgatcg ctctcgccct gctggctatt
     5581 gcgcagccac tttgcggaca ggcccagttc ctcaagactc tgaccaacaa tctcaaacaa
     5641 acactgcaaa accgggcgaa cggcaagtcc aaccagacca cgaacgtcat tctggataag
     5701 atcgatagcg ccacgcgggt ggggggaaca aaaaccggta agacgacggc gaccggggcc
     5761 cctgggacac agggaatgac gggaacgacg aatgctggcg gacagacagg gggactgccg
     5821 aatgtttttg gcgggtcggt cgataccagc gggctcagcc gggtgctggg cgcctttgca
     5881 aagacggcgc aggagaatcc gaacgacacc agtcaggcgg atctcgtgat gaagtccctg
     5941 ggccggttga caggcgggga tggcgtttca ccccaggatt cggcggcggc gatcaagagt
     6001 tttatgacgg ccagcggcgg cagcggtgtg ttgtatcaga cgatcacgac gactaccagc
     6061 aaacgcggaa atacgcgcga taccagctcg ctttggatga cgaacagcgg ggaagggcgg
     6121 tccgagatgc ggataccgat accgggggca gtgacgccga agtttgtggt gatcgggcgt
     6181 gcgaatcagc cgacctactc gatcatactg gatgcagcca acagaagcta ctcgctgaat
     6241 gtcatcgata cggcgctgat caattcagga attgaaaagt accaggtgac gcgggtaggg
     6301 acggagacgg tcgcgggcta ttcctgcatc cataccaaga tcgtccagac gacgggttcg
     6361 ggaatgttca aatcgacaac tgccatggaa ctgtggacca gtacgacggt tcccggctat
     6421 gcgatctata gcaagctgat cagtttccaa tcttccaacg ggggatttct tggcgcattg
     6481 aataaagcgg gcgcaggggg attcctggtc aaaatgacga cgggagacgg gaaggatgtt
     6541 tccatgacga tgcagctatg gaaggcagag caaaaaagct tcccggcttc gctcttcgcg
     6601 atccccgccg ggtataccaa cgatgagaag acgacggcgc agcggatgct atcgggctcc
     6661 gcaccggcca aacactgatc aatgccgagc catgacagct aaactagcca tatttctcct
     6721 gctttttcta ccggcaacgt atacacgtgc tcagaacctg gtggacctca gggaccagaa
     6781 accgttcacg ctgcacgggt cgctcggcgc aggaatggaa ttctataagt cgaatgagcc
     6841 atacccgacg cggcctccct ttgcatggaa tatctatgga agccttatgc cctcggtcta
     6901 tgggataagc ctcccgatgt ccttcgtggt cactcaatac ggaaacagct actcgaaccc
     6961 gtttacgcag atcgggatca gcccgtccta caagtggata cggctgcacc tgggatacag
     7021 aacgatccgg ttctccccgc tggtattcgc gggacagagc ttccttggcg gaggggtcga
     7081 gctgagcccg aaggggttct atttcggagc gttttatggc ttattgaaca aggccgtcca
     7141 ggaagatacc agcttcgccc atcccatcga gccgcaatat gcgcggatgg gctatggctt
     7201 caagatcggt gtgggcagcc cgaaaaataa tttcggcttt tcttttttcc atgctaaaga
     7261 tgacagcggg tcgatcagga ggatgcccga cagcctgacg acgatcctgc cacaagagaa
     7321 caccgtggtg ggaagctcat ggcattttac cttcttccac cggctgacct ggaccggcga
     7381 tctggcggcg ggactgctca accgcgatca gtcctatcag gagatcgaca gcgtggggta
     7441 ttacaagatc ccgaccctga tgcgcaagat catgccggtc aattggagct cggtattctc
     7501 ctatgccggt cagtcgcaat tgtcgtttac ctggaagacc gtcaatgcat cggcaggcta
     7561 tcggcggatc gcgccggatt tcgtctctct gggcgtaccg tatatgctga acgacctcga
     7621 aatgatgaac gggtcttttg gcggaaattt cgacaaagga cgactcaatg ccaatctcgc
     7681 gatcacgacg cagcataaca atctttcaca tctgctcgct tccaccctgc tcacgcggac
     7741 cgggaacctt tcgctgaatg ccttcgtgtc ccagcacttc aatatcaatg cgaccgtgac
     7801 cggcgtgcag gtgtatcaaa gggatggggc cgtggcgctg agcgattcgg tgcggatgaa
     7861 ccaattgatg atctccttta cactcgcgcc caccctgaat tttacgggca atgactatca
     7921 gcatacggtc aacgccaata tcagctatac cgacctcgac gatcacaatc cggcgacggc
     7981 ggcaatgacg ggcggcaaca atatcagtac gtccctgaat tatgcgctgt atttctccca
     8041 tgcgtattgg ggattgaatg cggcggtttt gtatagccag tacggacaag gggacaaccg
     8101 gtaccagtcg acagggatca atgccgggtt gaatgcccag ttattgagca gtcaccaatt
     8161 ggcgctgcag gggagcgcgg gatacttcct gaataagtat tccaattctc cgacgggcaa
     8221 caataccacg tttgcttttt ccggcaacta tacgatggcg cttcatacgt tgggggtgta
     8281 cctgaactat gtgctgacgc cgccggtcaa tctgaatccg ttgacgacga tctatcatat
     8341 cccgatcgcc gtgaactcga aaaatttcgc gggcggcgtc acctatggat tccactttta
     8401 aaatcgtcac atgatccgga tattcacctt ctgctccttg ttgctgctgt cgttgacagg
     8461 ttggacgcag tttacggtcg gcgtacaggt caatgtcgtg cagcccgtgc ctccttatct
     8521 accgcagctg aaggcagaca tcatgggcaa tcgtgccggc cagctgaacc aggatatcag
     8581 cagccatttg tcgatcatcc tccggtatac gggaaggact gctcagcggg tcaagctggc
     8641 ggggagtatc gagcggatat ccccggccgt gatgggcgtg tcgctgcggc ccgattttca
     8701 gcccgcgcag ccgatcgtgc tcggtccggg gcagccgata ctcagcctga cgcgcgatat
     8761 gttgcaaaat gcattcggaa atttcaccga aaactccctg gtctatacga attgcgacct
     8821 caataccttg cggcagaacg gcatcgattt caagctgccg gaaggcactt accgggtatg
     8881 cgtgactgct tatgattatg accggcccgg tttctcggcg cccttatcgg cgcccggtac
     8941 cggctgtgcg tatttcacga tctgttatac ggcttcggcg ccccagctcc ttttacccgt
     9001 gagcaccttt atcaatgcga ccggcggttt tcaggacgtc actccacatt ccaatctggt
     9061 acagttcacc tggacacccc cgtccacgac ctgcgggttg ccgatggggc ccctgaccta
     9121 cgagctggag atcaagcggg tatttaacgg gcagtcggtg atggatgcgg tcaataatcc
     9181 ctttgtcttt catcagcaaa atattcccgt cacgactttc ctgctggata cgctgaaata
     9241 tgggcatatc ctggtgccgg gccaacaata tatcgtccgc gtaaaggcta attttatccc
     9301 gatggtgggc agtcctctgg agatcgccaa ccagggctgg agcgagatcg gggcgttcga
     9361 ttgggcgcct gtctcttatt tcccaaaaac agcagttgcc gtcaaccacc cgacgcctga
     9421 caagccggtg ccgccgggtt cgatccagcc tggaggatat atcaatgagc catatacgcc
     9481 gatagcggct tgcgcggtgg cgacgccggt aagcaataca agcccattgc caggttcacc
     9541 cgcggggcaa caggtgacca tcgatggatt tttgatgcag atctcgcagg ctaccgcaaa
     9601 taccgatggc agctataaag gatcgggtac gatctcctgg catcccttct cgggcgacat
     9661 caggctgaat gtcagctttg acagtattcg cgtcaacacc gataaggtgg tatatgccgg
     9721 gacggcgcag acggtgagcg acaacgggta cccggactgg tccgtgtttg gtgcggccgg
     9781 ggcagacccc gtgacaaagc tcgcgggtct ggacgatggt gtgttgagcg ccatcaaaac
     9841 gaggatcggg gacgggtccc acctgctgaa tacgggtggc ggcggcccgg tgagcttccc
     9901 gatggggctt tccacgaccc tgggcggtgc gcccgccacg ctcgccgtga tggggatcag
     9961 tttccgctcg tcctgtacga atatggatgt gctcttcgat ctcaacctgc ccgatatcgg
    10021 cggctggctg gcgctggcgg gagcaggatt atcgatagga cccaattcgc ttttggcggg
    10081 gacaggcggg gttttgtatc tgccccagga caagagtttc gcggcgggtg ggatcaacta
    10141 tacgatcgat ggctgcccgg gggcgggcgc cagtgcggtg gatacctcca aaggaacata
    10201 tatagcctgg gatgcggtga aaggattgag cgcggttgtc gcgaatgccg acatccgttt
    10261 tacggatggc aatgcgatcg ttgccgtcga taaaaatgac cagcggctga cgactccagc
    10321 gactatacat gcccgtttcg ccttctccga ttggaacgat tgggtggcga cggccgtgtt
    10381 gcccaacgac ttcgaactgg cgggtctgcc gggtttcccg atccgttccg atgggttgtt
    10441 cttcgatcac tcgacgcggc agaacccggg tgggatcggc ttccccacgg gctacacagg
    10501 ggcggaggac gccggtttcg agggattgta tatcccggcg ctgtcgatgt cgttgccggc
    10561 aagttttcaa acgttctcgg gcgggaaacc tggggcgatc aattttaccg gtttcattct
    10621 cgacgacaac ggggtaacga caaacattaa tgccgccaat atcctcgata tcaatagcgg
    10681 tagtcttggg ggctgggcgt tttcgatcga taagataaat atcgcggtcg tgaataataa
    10741 tttccagagc gggatggcga tgtcggggca ggtccggttg ccggtcagca gtgacgggtt
    10801 atcctatacc tgtgctctga acagcgtgga cgggaaagtg gattaccggt ttgtggtaca
    10861 gccgggcggc ggactgaatg tgccgctgtg gctggcgaag ctgaatctgg atcccaactc
    10921 gtcgttggtg atcagcagcg atcctacggg gatggcagta aaaacgcatt tgaacgggaa
    10981 catcgggatc acgatcgacc attcgggaat gccgaagctg tcgttgcccg ctttgtcgtt
    11041 ccaggatatg gcgatggcga accgggctga tactagcgcg ggtgcgggtg cgggcttctt
    11101 tttttcgccg ggcaaatgga gccttggcgg cggggt
//
LOCUS       DASYSV010000504         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1001557, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000504 DASYSV010000000
VERSION     DASYSV010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001557"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..948
                     /locus_tag="VGQ51_15605"
     CDS             85..948
                     /locus_tag="VGQ51_15605"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEV9038060.1"
                     /translation="MRNFLLPSLILICLTTGAQQLHSRWDELTASDWPKALATSGRTC
                     ILPIGILEKHGPHVPIGSDLIQVREWAARATKKEYAVVFPDYFYGQINEARHVPGSFA
                     LPERVVWDLLEATCDEIARNGFDKIVIINGHGGNPELIRYFIQTRLGKRRNYAVYFFE
                     PSSDSIYRRQLAVLHRSDMAGDQHAGERETSSILYLRPDLVEMDSANNESGANQHRLS
                     IPNVYTAIWWYADFPNHYAGEGAKATKAEGELISEHTIAQLVEALKAIKADTKTLQLQ
                     KEYFDKVRTEP"
     gene            945..1937
                     /locus_tag="VGQ51_15610"
     CDS             945..1937
                     /locus_tag="VGQ51_15610"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEV9038061.1"
                     /translation="MTATTDAANAKARVDLLISCDWGTSAFRLRAVEDPSGKVVASTR
                     SDYGITAAFSDWQRSELPEPQRAGFYRSILYGAIDDLQRQHSLDLHGATILLSGMVSS
                     SLGIQELPYHPLPFDVSGADLRPAVLPATMDFPHTILLVPGIRSHDDVIRGEETQLIG
                     CRPPVEKQERVFIFPGTHSKHITIREGRAVAFTTYMTGEFFNLLAQRSLLAVSVAKPS
                     SAAVTTAFYAGIDAARDSSILRGAFSVRTNQLFKRLGKEDNYQYLSGLLIGEELKAIG
                     HRPITLVSSGPLLSSYQAALQYLGAPPIQTIDADNALIRGHAILWKATVVGKIH"
     gene            1960..2280
                     /gene="trxA"
                     /locus_tag="VGQ51_15615"
     CDS             1960..2280
                     /gene="trxA"
                     /locus_tag="VGQ51_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV9038062.1"
                     /translation="MATSEKRSFGEIIQGNKPVLVDFSAEWCGPCKMMPPILKEVKDK
                     MGESITILKMDIDRNPAVASAYGIQSVPTLIVFRDGQIKWRQSGVTRADQLKNVLDGI
                     LEQS"
     gene            2292..2774
                     /locus_tag="VGQ51_15620"
     CDS             2292..2774
                     /locus_tag="VGQ51_15620"
                     /inference="COORDINATES: protein motif:HMM:NF023255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HEV9038063.1"
                     /translation="MKILLLIAVLTASVVGHTQEPALSPVLAGYYGVKDALVKDDAKA
                     AAAAAKEMLTAVGGVNMSALPEKDHKAFMTLQNKLAYDARHISESSDIRHQREHFESL
                     SANMAALAKQAKLSQQPIYEDYCPMKKVYWLSNETAIHNPYYGSSMPTCGKVTSTLKP
                     "
     gene            2780..>4520
                     /locus_tag="VGQ51_15625"
     CDS             2780..>4520
                     /locus_tag="VGQ51_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEV9038064.1"
                     /translation="MTRKTCLFCWLVLCTWTYARAQDHPHTADRPQTMDRAQTADRPH
                     TVVYHLYISDTVVNYTGHPRHAIAVNGRLPGPTLEFTEGDTAEIYIHNGMMMETSVHW
                     HGVIVPNRYDGVSYLTSPPIRAMQTYVAKFPIVQHGTYWYHSHTMLQEQIGLYGAIVI
                     HPRPGTEPPVHEQVVLLSDWADEKPYEIQRSLHFGTDWYSIRKGSTQDYAAAIKAHSL
                     NAKLTSEWKRMFPMDVSDVYYDRFLVNGQPQEAATHYKPGDKIRLRIINGGSSTYFWL
                     QYSGGKMTVIASDGKEVDPVDVDRLIIGVSETYDVLVTVPDSGSFELRATAEDRTGST
                     SLWLGSGAKVTLKPLPPLRYFDGMRMMNGMMKGNGEMKETGMAMSNQTMDMNTVMYGE
                     SGGTTLNYGMLRSPTPTTLPEAPTRVFHFNLTGNMNRYVWTIDNKTVSESDKILIRHG
                     ENVRIILFNGTMMRHPMHLHGHFFRLLNGQGEYAPLKEVVDILPMETDTLEFAATESG
                     DWFFHCHILYHMMSGMGRIFHYEDSPPPPGIKDPARALKKLYSDDREIRPMARVGLES
                     NGSDGEFMLADTRY"
BASE COUNT         1071 a         1321 c         1204 g          924 t
ORIGIN      
        1 ggctacagag tggtcttctc gctggccgaa ctggattgtt tccaggtagc gtcgatctcc
       61 atccaatccg ctaaagacta atatatgaga aacttccttc ttccttcgct gatccttatt
      121 tgcctgacga ccggcgccca gcagctgcat agtcgctggg atgaattgac ggccagcgac
      181 tggcccaaag ccctggcaac ttccggccgt acctgtatcc tcccgatcgg tatcctggaa
      241 aaacatggac cgcacgtgcc gatcggcagc gatctgatcc aggtgagaga atgggccgcg
      301 cgggcgacga agaaggaata tgccgtcgtc ttccccgatt atttttatgg tcagatcaac
      361 gaggcccggc atgtgcccgg tagctttgcg cttcccgagc gcgtcgtttg ggatctcctc
      421 gaagccacct gtgacgagat cgcacgcaac ggtttcgaca agatcgtcat catcaacggt
      481 catggcggca accccgagct gatccgttat ttcattcaga cccggctggg aaaaagacgc
      541 aactatgcag tctatttctt cgaaccaagc tcggattcta tctaccggcg gcagctggcg
      601 gtactgcata gatccgatat ggcaggcgat cagcatgcgg gcgaacgcga gacgtcctca
      661 atactatatc tgcggcccga tctcgtcgag atggacagcg ccaacaacga gtccggcgca
      721 aaccagcacc ggctgagcat ccccaatgta tataccgcca tctggtggta tgccgatttt
      781 cccaatcatt atgccgggga aggagcaaaa gccacgaagg ccgagggcga gctgatcagc
      841 gagcatacga tcgcgcagct ggtagaagcc ctgaaagcca tcaaggccga taccaaaacc
      901 ctgcagctcc aaaaggaata tttcgacaaa gtccgcactg agccatgaca gccacgactg
      961 acgcggccaa cgctaaagcc cgtgtcgatc tgctgatcag ctgcgactgg ggaacttctg
     1021 ccttccggct gcgggcggtg gaagatcctt ccggcaaggt agtggcttcg acgcgaagcg
     1081 actacgggat aaccgctgca ttttcggact ggcaacggag cgagctgccc gaaccgcaac
     1141 gcgccggctt ttaccgaagc atcctgtacg gcgcgatcga cgatctccaa cggcagcatt
     1201 cgctggacct gcatggcgcg acgatccttc tgtcgggcat ggtatcctcc tcgctgggca
     1261 tccaggaatt gccttaccac ccccttccat tcgatgtctc gggtgccgat ctgcgacccg
     1321 cggtccttcc ggccaccatg gatttcccgc ataccatatt gctggtaccc ggaattcggt
     1381 cacacgatga cgttatacgc ggcgaggaaa cgcagctcat cggctgccgc ccgccggtag
     1441 aaaagcaaga acgtgttttt attttccccg gcacacattc caaacacatt accattcgcg
     1501 agggccgggc cgtcgcattc actacctata tgaccgggga atttttcaac ctgctggctc
     1561 agcgaagcct gcttgccgtc tcggtcgcca aaccttcgtc cgccgcggta accaccgctt
     1621 tttatgcggg gatcgatgcc gcacgcgact cgtccatcct gcgcggcgcc ttctcggtcc
     1681 gaaccaacca gctgttcaaa cggctgggta aagaagacaa ctatcaatat ctcagcggtc
     1741 tgctcatcgg ggaagagctc aaagccatag gtcatcgccc gatcaccctc gtctcgtcgg
     1801 gaccgttgct ttcatcctac caggctgcct tgcaatatct cggcgccccg ccgatacaaa
     1861 ccatcgacgc cgataacgcg ctgatccgcg ggcatgcgat cctctggaag gcaacggtag
     1921 tcggcaaaat tcactaactt tacaggaaat cgagcgtata tggcaacaag tgaaaagagg
     1981 tccttcggcg agatcatcca gggcaataaa cctgtcctcg tggatttctc ggcggaatgg
     2041 tgcggtccct gcaaaatgat gccgccgata ctcaaggaag tcaaggacaa aatgggtgaa
     2101 agtatcacca tcctgaaaat ggacatcgat cgcaatcccg ccgtcgcatc ggcatacggg
     2161 atacaaagcg ttcccacgct gatcgtcttc cgcgatggcc agatcaaatg gcggcagagt
     2221 ggagtgaccc gcgcagatca attgaaaaat gtgctcgacg gcatcctcga acaaagttga
     2281 atttattgaa tatgaaaata ttgcttctga ttgccgtcct gacggcaagc gttgttggac
     2341 atacacagga acccgctctg tcacctgtcc tcgccggcta ttacggcgtc aaggatgccc
     2401 tggtgaagga cgatgccaaa gccgccgcgg ctgccgccaa agagatgctg actgccgtgg
     2461 gtggggtgaa catgtccgcg ctgcccgaga aggatcataa ggccttcatg accctgcaga
     2521 ataagctggc ctacgacgcc cggcatatct cggagtccag cgatatcagg catcagcgcg
     2581 agcattttga gagcctgtct gcgaatatgg ccgccctggc caaacaggca aaattgtcgc
     2641 agcagcccat ctatgaggac tattgcccga tgaagaaggt ctattggctc agcaacgaaa
     2701 cggcgatcca caatccctac tacggctcca gcatgcccac gtgcggaaag gtcaccagca
     2761 ccctgaaacc ataagacctg tgaccaggaa aacctgtttg ttctgctggc tggtgctgtg
     2821 tacctggaca tacgcccgcg cccaggatca tcctcatacc gcggatcgtc ctcaaaccat
     2881 ggatcgcgct caaaccgcgg atcgcccaca tacggtcgtc tatcacctgt acatcagcga
     2941 tacggtcgtc aattataccg gtcatccccg ccatgccatc gccgtcaatg gccggttgcc
     3001 gggccccacg ctcgagttta cggaaggcga taccgccgag atatatattc acaacgggat
     3061 gatgatggaa acctcggtcc actggcatgg ggtgatcgtg ccgaatcgat atgacggagt
     3121 gtcctatctg acctctccgc ccatccgcgc gatgcagacc tatgtcgcga aattcccgat
     3181 cgttcagcac ggaacgtact ggtatcattc ccatacgatg ttacaggagc agatcgggct
     3241 atatggggcg atcgtcattc atccgcgccc cggaaccgaa ccgccggtgc acgagcaggt
     3301 cgtccttcta agcgactggg cggacgaaaa gccctatgag atccaacgca gcctgcattt
     3361 cggtacagac tggtattcca tccggaaagg cagcacacag gattatgccg cggcgataaa
     3421 agcgcacagc ctgaatgcta aactgaccag cgaatggaaa cgtatgtttc ccatggatgt
     3481 cagcgacgtg tattatgacc ggtttctcgt caacggacaa ccgcaggaag ccgcaacgca
     3541 ctataaaccc ggcgacaaaa tacgtctgcg catcatcaac ggcggatcat ccacctattt
     3601 ctggttacag tattccggcg gcaaaatgac ggtcatagcc agcgacggga aagaagtcga
     3661 tcccgtggat gtagaccgtc tcatcatcgg agtgtccgaa acctacgatg tgctggtcac
     3721 cgtgcccgac tcgggcagct tcgaattgcg ggcgaccgcc gaagatcgta ccggcagtac
     3781 ctctctttgg ctgggcagcg gcgcaaaggt gaccttaaaa ccactgcctc ccctgcgcta
     3841 tttcgatggc atgcggatga tgaacgggat gatgaagggc aacggtgaaa tgaaagagac
     3901 cggcatggcg atgagcaacc agaccatgga tatgaatact gtcatgtatg gcgaatcggg
     3961 cggcaccacg ctcaattatg ggatgcttcg ctcgccgacg ccgacgacgc ttccggaagc
     4021 gccgacacgg gtctttcatt tcaacctcac agggaacatg aaccgttacg tctggaccat
     4081 cgacaacaag accgtctccg agtcggataa gatactgatc cgtcatggag agaacgtacg
     4141 gatcatcctg ttcaacggca cgatgatgcg tcatcccatg catctgcacg gccatttttt
     4201 ccggctgctc aacggtcagg gcgaatatgc gcccctaaaa gaagtggtag acattctgcc
     4261 catggagacg gatacgctcg aatttgccgc tacggaaagc ggcgactggt tttttcattg
     4321 tcatatcctc tatcatatga tgagcggaat gggccgtatt tttcattatg aagactcccc
     4381 gcctcccccc ggaattaaag atcccgcccg cgcactgaaa aagctataca gcgatgaccg
     4441 ggaaattcgt cccatggcgc gcgtaggcct ggaaagcaat ggcagcgacg gagagtttat
     4501 gctggcggat acccgctatc
//
LOCUS       DASYSV010000505         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2410903, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000505 DASYSV010000000
VERSION     DASYSV010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410903"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VGQ51_15630"
     CDS             <1..1024
                     /locus_tag="VGQ51_15630"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV9038065.1"
                     /translation="MKYKLLGRSGLKISELCLGTMGFGTESGWGADRSTSFDIMDAFA
                     NAGGNFLDTANIYKLGTSEKIIGEFIGTRDRDYFVIATKYSLKDNTTNPNASGNNRKN
                     MMRSVEHSLRRLQTGFIDVLYLHIWDDLTPIDEVLRGIDDLIRQGKVNYAAISDTPAW
                     VVSKGNTLAELKGWSQFIALQVEYSLLQRSPERDLIPMAKHFGMTVTPWAPLAGGALT
                     GKYLRGGQGRVKAESNRRDENSTRITREVMAIAEQRGVEPSHVALQWTRQQGFDCIPI
                     VGATRPDQLQENLRAIDLTLSEHDLRKLDAASAIDLGFPGKFFREDGVRQNNFGGFYD
                     RIIRRP"
     gene            complement(1082..1501)
                     /locus_tag="VGQ51_15635"
     CDS             complement(1082..1501)
                     /locus_tag="VGQ51_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEV9038066.1"
                     /translation="MTKDLKRFIAAQERDYPVALAEIRAGRKRSHWMWYIFPQLEGLG
                     HSPMAQRYAISGAEEAAAYLADDVLGPRLVEISRALMGLAGSDATAVMGTPDDLKLWS
                     CMTLFSMVPGADKVFSEVLGKYYGGRRDERTVEMLRH"
     gene            1579..1770
                     /locus_tag="VGQ51_15640"
     CDS             1579..1770
                     /locus_tag="VGQ51_15640"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEV9038067.1"
                     /translation="MRTNIELNDVLVKQAIRLSKLKTKREVVEEALKNYVAAMKKKEL
                     LKLKGRVSWEGNLKDMRRL"
     gene            1767..2183
                     /locus_tag="VGQ51_15645"
     CDS             1767..2183
                     /locus_tag="VGQ51_15645"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEV9038068.1"
                     /translation="MNEPLIFDTSVWIDFFIGKNDARTNLLTSYIEQEDQILLIPTIL
                     QEVLQGIREDAQYRQVKEILSYFTVLQLPPVQAAIGAAELYRGLRKKGVTIRKSNDCL
                     IAFYAIEFSAPIVHSDNDFELIGKHSKLNTWKAPAR"
     gene            complement(2250..>2470)
                     /locus_tag="VGQ51_15650"
     CDS             complement(2250..>2470)
                     /locus_tag="VGQ51_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038069.1"
                     /translation="CSFILQRFESKGFAENFMLSVRISDAKEFHEMVIGKKLAERFGI
                     RVGNVSQQPYGKEVNIIDIAGVCWHFIE"
BASE COUNT          654 a          773 c          532 g          511 t
ORIGIN      
        1 catgaaatac aaactcctcg gacgtagcgg tctcaaaatc tccgaacttt gtcttggcac
       61 catgggcttc ggtactgaat ccggatgggg tgcggacaga tccaccagtt tcgacatcat
      121 ggacgccttc gccaatgccg gcggtaattt cctcgacacc gccaacatct ataagctggg
      181 caccagcgaa aagatcatcg gcgaattcat cggcacccgc gaccgcgatt atttcgtcat
      241 cgccaccaaa tattcgctga aagataatac taccaacccc aacgccagcg gcaacaaccg
      301 gaagaacatg atgcgcagcg tcgagcatag ccttagacgt ctccaaaccg gcttcatcga
      361 cgtgctatat cttcacatct gggatgatct cacccccatc gacgaagtgc tcaggggcat
      421 cgacgacctc atccgtcagg gcaaggtcaa ctatgcggcc atcagcgaca ctcccgcatg
      481 ggtcgtgagc aagggcaata cgctcgccga gctgaaaggc tggagccaat tcatcgcctt
      541 acaggtcgaa tacagcctcc tgcaacgcag ccccgaacgc gacctgatcc ccatggccaa
      601 acatttcggt atgacggtga ctccatgggc gcccctggcc ggcggcgcgc ttaccggcaa
      661 gtacctgcgt ggtggtcagg gccgcgtcaa agccgagagc aatcgccgcg acgaaaacag
      721 tacacgcatc acccgcgagg tcatggccat cgccgaacag cggggcgtcg agccgagcca
      781 cgtagcgctt cagtggaccc gccagcaagg cttcgactgt attcccatcg taggcgctac
      841 tcgtcctgat cagctgcagg aaaacctccg cgctatcgat ctcacccttt ccgaacacga
      901 tctccggaaa ctcgacgccg ccagcgccat cgatctcggc ttccccggca aattcttccg
      961 cgaagacggc gtccgccaaa ataatttcgg cggcttttac gatcgcatca tccgccgccc
     1021 ctagcgctga tcgctcatcc atccgccgcc cctggcgctg ttcactcatc atccgccgcc
     1081 cctaatgtcg caacatctcc accgtccttt catctctccg tcccccataa tatttcccaa
     1141 gcacctccga aaaaacctta tcggcgcccg gtaccatcga gaatagcgtc atacacgacc
     1201 ataacttcag atcatccggc gtccccatga ccgccgtcgc atcactacct gccaatccca
     1261 tcaacgcccg cgatatctcc accagccgcg gccccagcac atcatcggcc agatatgccg
     1321 cagcctcctc cgcaccactg atcgcatatc gctgcgccat cggactatgc cccaacccct
     1381 ccagctgcgg aaaaatatac cacatccagt gactccgctt cctcccggcg cggatctcgg
     1441 ccagcgccac gggataatcc cgctcctgtg ctgcgatgaa tcgtttcaaa tctttcgtca
     1501 tatacccaaa caaattacaa atctaattcg ttacaatgtg tataattttt attattttta
     1561 cacataaagt caaaagacat gagaacaaat atcgaactga atgacgttct tgtaaagcaa
     1621 gccatcaggc tgagcaagct taagacgaaa agggaagtcg ttgaagaagc cctcaaaaac
     1681 tatgttgcgg ccatgaaaaa aaaggaattg ctcaagctaa aaggccgggt ctcctgggaa
     1741 gggaacttaa aggacatgcg taggttatga acgagccctt aatattcgac acttcagtgt
     1801 ggattgactt tttcatcggt aaaaacgatg caaggaccaa tctgctaacc tcctacattg
     1861 agcaggaaga tcagatatta ttaattccca ccatcttgca ggaagtcctg cagggcattc
     1921 gcgaagatgc acaataccgg caggtcaaag agatattatc ctatttcacc gtattacaac
     1981 ttccgccggt acaggcagca atcggcgccg cagaattgta cagaggctta cggaaaaaag
     2041 gggtaactat tcgaaaaagc aatgactgcc tgattgcctt ttatgccatt gaattctccg
     2101 caccgatagt tcactcggac aacgattttg agctgatcgg caagcattca aagctcaaca
     2161 cctggaaagc cccggcccga tagatggagc gatcccaccg gaagacccgg cgccacggcc
     2221 agcacttcca caaaaccaaa tccattatct tattcaataa aatgccagca gacccctgcg
     2281 atatcaataa tattcacttc ctttccataa ggctgctgag aaacatttcc aacccgaatg
     2341 ccaaaccgct ccgctaactt cttcccgatc accatctcat gaaattcctt cgcatcgctg
     2401 atcctcaccg aaagcatgaa gttctccgca aatcccttcg actcgaatcg ctgtagaatg
     2461 aaagaacacc
//
LOCUS       DASYSV010000506         5423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1387900, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000506 DASYSV010000000
VERSION     DASYSV010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387900"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VGQ51_15655"
     CDS             complement(<1..436)
                     /locus_tag="VGQ51_15655"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEV9038070.1"
                     /translation="MKEINFSRTIIIVSIAIPAMVAFLILAPGSKIDPGFNTRNLPLL
                     HAILNATTAILLLLSLYFIRHGQVKAHKRTNWTAITLSAIFLVSYVIYHASNPSVRYG
                     DLNHDGFLSAAEKVQAGSMRYVYYVVLSSHIILSAVIIPFVLF"
     gene            complement(442..1239)
                     /locus_tag="VGQ51_15660"
     CDS             complement(442..1239)
                     /locus_tag="VGQ51_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEV9038071.1"
                     /translation="MSKKGWLAICIAVIIPLMFYGIASQYGVVMPKHFFPDSVVTRVK
                     DGKEITDTIWHKVANITLQNQLGDTVSLDQLKGKVIIIDFFFTHCASICPILTHNMRR
                     LQDALKLRDDMKGVDTSFVQFLSLTVDPAHDSPPVLKKYADRYDVNSDVWWMLTGPKK
                     TIYDFALNELKLGLQDSVSVDSNFVHTDYVALLDKNRVIRGYYHGTDTTAMARLGDDL
                     VFIMLEKDKNRKSPVFAELKPLIVPIAIILILTALVVVYFSRKPKFS"
     gene            complement(1324..1779)
                     /locus_tag="VGQ51_15665"
     CDS             complement(1324..1779)
                     /locus_tag="VGQ51_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEV9038072.1"
                     /translation="MSSNTTHDNPQAVGHDNAHAHFDTRPIWRTFWVLLGITLVELTL
                     ATIHMKSEFPPKYVLNGIYLILTLAKAFYIIAEFMHLRHEIKNLIMSIAIPALLFIWF
                     LLAFALDGNSYRHLRNRYDPYHVEQSHTKAPSPEGEHVTPPAQKPGTPQ"
     gene            complement(1798..2472)
                     /locus_tag="VGQ51_15670"
     CDS             complement(1798..2472)
                     /locus_tag="VGQ51_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEV9038073.1"
                     /translation="MAQAVPTGTKWWSGGKSPYNIEYGKLMMWYFLMSDAFTFGAFLI
                     SYGTIRVSSNGWPDPNEAFKAFPGLEGINLPLVFVSLMTFILIMSSVTMVLAVAAGHN
                     GDRKKVTFYMILTIIGGLAFLSCQAWEWTHLHGEGAWWGRNPFMNADGTASSTNFTNF
                     FFTITGFHGFHVFSGVCVNVIMLIKNIRGDFDKKGHYLMIEKAGLYWHFVDLVWVFVF
                     TCFYLL"
     gene            complement(2515..3078)
                     /locus_tag="VGQ51_15675"
     CDS             complement(2515..3078)
                     /locus_tag="VGQ51_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEV9038074.1"
                     /translation="MEIVNEQRQKIHPHKFTLWVALGSIIMMFAGLTSAYVVKRDQPG
                     WSTFSIPRAFYYSTAVILASSLTVQMALKAFKDREMIRYRNLLSTTAVLGVAFVLLQW
                     KGFREIWSTGITLHGSGGGQFLYIIAGLHAVHVLGGVVALLVMVARAFMPRIRSYNSV
                     PTELMSTYWHFVDLLWIYLFIFFALIK"
     gene            complement(3114..3995)
                     /gene="cyoE"
                     /locus_tag="VGQ51_15680"
     CDS             complement(3114..3995)
                     /gene="cyoE"
                     /locus_tag="VGQ51_15680"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEV9038075.1"
                     /translation="MKQTSSLNFAGKLQDYKLLIKFNLSFMVVFSALISYLLAPKVKE
                     FDWAAIGLFFIGGMLVTGSANAINQIVEKDTDALMKRTAKRPVASGRMSTQEAWTFAV
                     VAGIGGVLILWHFNFLAAALAAFSLFLYAFVYTPLKKVSSISVLVGAVPGSLPCLIGW
                     AAGNDSLSAGGWVLFALQFFWQFPHFWAIAWIAHKDYTAAGFKLLPSNEGPTKYSAIQ
                     AVIYSLLLIPVGVLPYLVGMSGVISLVIVLVANLGMVLQCVRLYREMTVAAARRVMFG
                     SYIYLPVVLLSLLANKL"
     gene            complement(4087..>5423)
                     /locus_tag="VGQ51_15685"
     CDS             complement(4087..>5423)
                     /locus_tag="VGQ51_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEV9038076.1"
                     /translation="VETGPASGGWTSYVPLSALGDASEGSKTGMDLWLISMATFVVSS
                     LLGGLNYISTILNMRTKGMSMTRLPLTIWALFFTAILGVLSFPVLLSGFILLLFDRHA
                     GTSFYLSDIFLGETGKALPNEGGSAILYQHLFWFLGHPEVYIIILPAMGLVSEVLSVN
                     ARKPIFGYMAMVGSLFAIAILAFLVWAHHMFVTGLNPFLGSVFVLLTLLIAVPSAIKV
                     FNWLTTLWKGNLRFTVGMLFAIGFVSTFISGGLTGIFLGNSTLDIQLHDTYFVVAHFH
                     IVMGVSGVLGMFCGIYHWFPKMYGRYMNNTLGYIHFWVTFAGAYLIFWPMHYEGLAGM
                     PRRYLDYSNWISFNQFGDLNRFISTVAIITFAVQLLFVFNFFYSIFKGRRVMTRNPWG
                     ATTLEWTAPIRPGHGNWPGEIPEVHRWPYDYAKDGRDFIPQTEPLGSNESVH"
BASE COUNT         1391 a         1435 c         1431 g         1166 t
ORIGIN      
        1 taaacaaaac gaacggaatg atcacggccg acagtatgat atgactgctc agaacgacat
       61 aataaacata tcgcatcgat cccgcctgca ctttttccgc cgcgctcagg aagccgtcat
      121 gattcagatc gccatagcga acggaaggat tggaagcgtg atagatcaca tacgaaacga
      181 gaaagatcgc gctgagcgtg atcgccgtcc agttggtacg tttatgcgct tttacctgac
      241 catgacggat aaaatacagg ctcaggagca ggagaatggc cgtagtagcg ttcaggatcg
      301 catgaagaag cgggagattg cgggtgttga agccgggatc gatcttgctg ccgggagcca
      361 ggatgagaaa ggccaccatg gcggggatgg cgattgaaac gatgatgatg gtccgcgaga
      421 aattgatctc tttcatagga tttacgaaaa tttgggcttt cggctaaagt agactacgac
      481 aagcgccgta agaatgagga tgatcgcgat cggcactatc aggggtttca attcggcaaa
      541 gaccggcgat ttcctgttct tgtccttttc cagcatgatg aaaaccagat cgtcgccgag
      601 acgcgccatc gcggtcgtat cggtcccatg ataataaccg cggatcaccc ggttcttgtc
      661 cagcagcgcc acatagtctg tatgtacgaa attcgaatcg acgcttacgc tatcctgcag
      721 acccagcttc agctcattga gcgcaaaatc gtagatcgtc tttttcggac cggtcagcat
      781 ccaccacaca tccgaattga cgtcatatcg gtccgcatat tttttcagga cgggcgggga
      841 atcatgcgca ggatccactg tcagggaaag aaattgcacg aagctggtgt cgacgccctt
      901 catatcgtcc ctcaatttga gtgcatcctg caggcggcgc atattgtgcg tcaatatcgg
      961 gcagatactc gcgcaatgtg taaagaaaaa atcgataatg atgacctttc ccttcagctg
     1021 atcgagcgaa acggtatcgc ccaattggtt ctgcagcgtg atgttcgcca ccttgtgcca
     1081 gatggtatcg gtgatctctt tgccatcctt tacgcgggtg acgacactat cggggaaaaa
     1141 gtgtttaggc attaccaccc cgtattggct cgctattccg tagaacatca gcggtatgat
     1201 gaccgcgata cagattgcca accatccttt cttgctcatg gaccacaaag gtaaaacaaa
     1261 aaatgggccc catttagtta aaagtaaggg gcccattttt tcacaatgac atctaagata
     1321 ttgttattgc ggtgttccgg gtttctgtgc cggcggggtc acgtgttctc cttccgggct
     1381 cggagccttc gtatgactct gctcgacatg ataaggatca tagcggttgc gcagatgacg
     1441 gtaggagttg ccgtccaggg cgaaagcgag caggaaccag atgaagagga gtgccggaat
     1501 ggcgatcgac atgatcaggt tcttgatctc atgacgcagg tgcatgaatt cggcgatgat
     1561 atagaaggcc ttcgccagcg tgaggatcag gtagatgcca ttgagcacat atttcggagg
     1621 gaactccgac ttcatgtgaa tggtcgccag ggtcaattcg accagcgtta tccccagcag
     1681 gacccagaag gtcctccaga tcggtctggt gtcgaaatgg gcatgggcgt tatcgtggcc
     1741 cactgcctgc ggattatcat gcgtcgtatt agatgacata taatgcgttt tgttgaatta
     1801 gagtagataa aaacaggtga atacgaatac ccataccagg tcgacgaagt gccagtacag
     1861 accggctttt tcgatcatga ggtagtggcc ttttttatcg aagtctcccc gtatattctt
     1921 gatcagcatg atcacgttta cacaaacccc ggaaaatacg tggaaaccgt ggaaaccggt
     1981 gatcgtgaaa aagaagttcg tgaaattcgt ggatgaagcg gtaccgtcgg cattcatgaa
     2041 ggggttccgt ccccaccagg cgccttcacc gtggaggtgc gtccattccc aggcctgaca
     2101 gctcaggaag gcgagaccgc cgatgatcgt aaggatcata tagaaggtta cctttttccg
     2161 gtcgccattg tgacccgcag ccactgccaa taccatcgtc acggagctca tgatcaggat
     2221 aaaggtcatc aggctcacga ataccagcgg aagatttatg ccctcgagcc cggggaatgc
     2281 tttgaaggcc tcattagggt cgggccagcc attgctggaa acccggatcg tgccgtagga
     2341 aatcagaaaa gcgccaaatg tgaaagcatc gctcatcagg aaataccaca tcatcagctt
     2401 cccatactct atgttgtaag ggcttttccc gccgctccac catttcgttc ctgtcggtac
     2461 tgcttgtgcc atattaggtc aattaattta cgaatttatt aactatactg aatatcattt
     2521 tataagcgca aagaaaatga acaaatagat ccagagcaga tcgacaaaat gccagtacgt
     2581 actcatcagc tcggtcggga cgctgttata gctacggatc ctcggcatga aggccctggc
     2641 gaccatgacc agcagcgcga cgacgcctcc cagcacgtgc accgcatgca gcccggcgat
     2701 gatatacagg aactgcccgc caccggaccc gtgaagggtg atgccggtgc tccagatctc
     2761 ccgaaagcct ttccactgca gcaatacgaa cgcgacccct aagaccgccg tagtcgacaa
     2821 caggttccgg taacggatca tctccctgtc cttgaaggcc ttcagcgcca tttggaccgt
     2881 gagactgctg gccaggatca ccgccgtcga ataatagaag gccctgggta tcgaaaacgt
     2941 actccacccc ggctggtcgc gtttcactac ataagcgctg gtcaacccgg caaacatcat
     3001 gataatgcta cccagcgcca cccataaagt aaatttatgg ggatgtattt tctgtcgttg
     3061 ctcgttcaca atttccatat atatttttca cgccgcctgt ggcggctctc tcctcacaat
     3121 ttattagcta acaatgacag caacactacc ggtaggtaga tatagctgcc gaacattact
     3181 cttctcgccg ccgcgaccgt catctcccgg tagagacgca cgcattgcag caccattcca
     3241 agattcgcca ccagtactat gaccaggctg atcaccccgc tcatgccgac aagataaggc
     3301 aacaccccta ccggtatcag cagcagcgaa tagatcactg cctgtatggc ggaatacttc
     3361 gtcggacctt cattggaagg cagtaatttg aaacccgccg cggtatagtc cttatgggca
     3421 atccaggcta tcgcccagaa atgggggaat tgccagaaga attgcaacgc aaacaggacc
     3481 cagccgcccg ccgaaaggct gtcattccct gccgcccagc cgatgagaca gggcaggctg
     3541 ccggggacag cgccgaccag caccgatatg gagctgacct tcttcagcgg cgtatacaca
     3601 aacgcataaa ggaacaggct gaaagccgcc agtgccgccg cgagaaagtt gaaatgccag
     3661 agaatgagca ccccgccgat ccctgccacc acggcaaagg tccaggcttc ctgcgtactc
     3721 atccgtcccg acgccacggg ccgtttggcc gtccgtttca tcagggcgtc ggtatccttt
     3781 tcgacgatct ggttgatcgc gttggcgctc cctgtcacca gcatgccgcc gatgaagaaa
     3841 aggccgatcg ccgcccagtc aaattccttt actttcggcg ccagcaaata gctgatcaag
     3901 gcggagaaga caaccatgaa gctcaggttg aacttgatca gcagtttgta atcctgcaat
     3961 ttgccggcaa aatttaaaga gctcgtttgc ttcatatata tgtgctgaag atgcgctgta
     4021 cgaaaattag cgcatctcca gcaaagcgct atttgtctgt cccgtggacg agccgccgtg
     4081 gcggctctag tgcacacttt cattgctacc cagcggttcc gtctggggaa tgaaatccct
     4141 gccgtccttg gcatagtcat agggccagcg atggacttcg gggatctctc cgggccagtt
     4201 gccatgtccg ggacgtatcg gcgccgtcca ttccagcgtc gtcgcacccc aggggttcct
     4261 ggtcattacc ctccttcctt tgaagatgga gtaaaagaag ttgaatacga acagcagctg
     4321 aacggcgaag gtgatgatag ccaccgtcga gatgaatcgg ttcagatcgc cgaactgatt
     4381 gaaggagatc cagttgctgt agtccaggta acggcggggc ataccggcca atccttcata
     4441 gtgcatcggc cagaagatga gataagcgcc tgcgaacgtc acccagaagt ggatataacc
     4501 caacgtattg ttcatatagc ggccgtacat cttgggaaac cagtgataga tgccgcaaaa
     4561 cataccgagc accccggaaa cgcccatcac gatgtggaag tgagcgacta cgaaataggt
     4621 atcgtggagc tggatatcca gggtggagtt acccaggaag atcccggtaa ggccacctga
     4681 gatgaaggtg ctcacgaacc cgatggcgaa aagcatccct accgtgaacc ggagattgcc
     4741 cttccacaac gtcgtcagcc agttgaacac cttgatggcc gacggaacgg cgatcagcag
     4801 cgtcagcaat acgaataccg agcccaggaa gggattcaga ccggtgacaa acatatggtg
     4861 tgcccatacg aggaaggcca ggatagcgat cgcaaacagt gagccgacca tggccatata
     4921 tccaaagatg ggtttgcgtg cattgaccga aagcacctcg gacacgagac ccatcgcggg
     4981 caggataata atatatactt cggggtgacc caggaaccag aacaggtgct gatagaggat
     5041 cgcactgccg ccttcattgg gcagggcctt gccggtctcg cccaggaaga tatcggacag
     5101 atagaagctc gtaccggcat ggcggtcgaa cagcagcagg atgaaaccgg acagcagtac
     5161 cgggaaggac aatacgccta agatggccgt gaagaacaat gcccagatgg tcagcggaag
     5221 acgggtcatg ctcatgccct tggtccgcat gttcagtatg gttgaaatat agttgagccc
     5281 acccagcagc gaggatacca cgaacgtcgc catagaaatg agccagaggt ccatccccgt
     5341 cttcgagcct tcgcttgcat ctcccagggc gcttaaggga acataagagg tccatccgcc
     5401 gctggcaggg cccgtttcga cga
//
LOCUS       DASYSV010000507        24471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2151546, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000507 DASYSV010000000
VERSION     DASYSV010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 24471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24471
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151546"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2178
                     /locus_tag="VGQ51_15690"
     CDS             <1..2178
                     /locus_tag="VGQ51_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038077.1"
                     /translation="SKITGEYNVNVATRSRHYSGEPTGDVHPAMLAITADMYSLQNTI
                     LPTGYTFSIVDAKGDILFDARPGRALLSNIFVEAGDNTDLQQCVRYRYERFFPNITLR
                     GREVALLSKPMAGTNYSLLVYYNLWAMQEVHFHSLGLSCFCMSCILLLIALVAFINEW
                     GKKRPSLLRLSKVNFDWLRPVPAKYGYYRHLIIWLRIFLALFLVAWVFIEMLLPEYEP
                     ALLLISLSFPIFIAVHYFVLREKYYHIERGEAIILPPALAPLTVALLAIFIYVCTAGL
                     GAGVIVTTLVVLVLFLGTILESWRRFGRTIANHGVKALLEKYTWAILHGVFLITLVPA
                     IALFILFYKEEHNGITRMGMLSMMQKIDDRARTIDSKKNLYLRDDNPDFMRASKFRNG
                     IYFTDRSADTPALFSEPVKTNFTAYSTLHNLLFQPDSTSLEALARLNAASDGSWQFAT
                     VGHPKRLVLDHCNPVGSCKNAFSLSSDPVTLYPALRFFLYRFLSLGAMRIFLLLLGIF
                     ALMLIAQRLTASLASHVFLLRLLEKYGRANYDEARLPEVLYPPVEENTTLHMQVRLEK
                     PYDDLWKKLTTEEKYVLYDFALDGFTNYKAGILIYKLLHRDLLYVDRNFLLRIRSADF
                     HNYLLSKDIFNPYQENKEDIKVYKYMMSVRKQGFWQAFRVPLQVILGAAGLFIFFTQE
                     ALSQKIIGLLTSLPFITQMLASFFERSNGQKNKDPGSDQNPDL"
     gene            2182..3288
                     /locus_tag="VGQ51_15695"
     CDS             2182..3288
                     /locus_tag="VGQ51_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038078.1"
                     /translation="MHFTDPLPNPDESNKPSRKKPIFPVNEGLRSYLKQHGREVRLPV
                     SYQDLLLHITYSVPLKDKNQQDTLWETALYDMRHWEFIREGLVQMYAILKTEGDLTFT
                     KHLDVARIDFCSFANSHPFRIRIVNKFNDNYDHYYIKQADASRIYGLELEHLLSPNRI
                     TFFTHDNTLVEEHIPGIPGDLFIRNFLEDTQTNKIRLSKEFVKFNERCFVRLLGDMRA
                     YNFVVDITPDIEDYQYRIRAIDFDQQSYEGRKNLYIPQFFKENRPFVELALQHLNKDS
                     IEQYQTEERTMMAFRVASSRFRLMDLLNIMSRDKPLSSPEKLLELKTGLAEFFGNPAF
                     LKCRSIGQVVKRQLKQTLQKNLSLLQKNLGKFED"
     gene            3357..4424
                     /gene="fbaA"
                     /locus_tag="VGQ51_15700"
     CDS             3357..4424
                     /gene="fbaA"
                     /locus_tag="VGQ51_15700"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEV9038079.1"
                     /translation="MAKYRSGVLFGDELEALYKDAKDNQFALPAVNTIGTNTINATLE
                     TAAKVNSPVIIQFSNGGAQFIAGKGMPNDKLQGNISGGVSGALHIHNVAKYYGVPVVL
                     HTDHASKKWLPWISGLIDAGEQFFREKKQPLFSSHMLDLSEEPIEENIHTSVDFFRRM
                     KPLGMAIEIELGVTGGEEDGVDNSGVENEKLYTQPQHVAYAYKELSKIGPLFSVAAAF
                     GNVHGVYSPGNVELRPVILKNSQDYIEKELKTGPKPVYFVFHGGSGSPQHQIREAIGY
                     GAIKMNIDTDLQWAYWEGVLQFYKKNEAYLQGQLGNPEGSDKPNKKYYDPRVWQRKAE
                     ENFVKRLEVAFQDLNCINRNA"
     gene            4560..5624
                     /locus_tag="VGQ51_15705"
     CDS             4560..5624
                     /locus_tag="VGQ51_15705"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEV9038080.1"
                     /translation="MPSKKILDALGDKASFLLDHKPVLDKSVLSAFPSPEHVEEIWVN
                     SDRNNQVLRSIQVLIGHGRLADTGYLSIFPVDQGVEHSGGASFAANPMYFDPENIIRL
                     AIEAGCSAVASTFGVLSIMSRKYAHRIPFLVKLNHNEFLSLPNRYDQTMYGTVKHAWN
                     MGAVAVGATVYWGSAESDRQLKEVAEAFEQAHELGMATVLWCYTRNPGFKVDGVDYHT
                     SADFTGQANHLGVTLRADIIKQKLPTNNGGYLATKHGKTSPLVYSKLTHPDNHPIDLT
                     RYQVLNCYSGRVGMINSGGESKGASDTADAVFTAVVNKRAGGMGMIMGRKAFQRPFKE
                     GVDIIQMVQDVYLDKDITIA"
     gene            complement(5648..6877)
                     /locus_tag="VGQ51_15710"
     CDS             complement(5648..6877)
                     /locus_tag="VGQ51_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038081.1"
                     /translation="MPKFFAVVLCCCWIIRLSAQNPTGTLVPPDKLVNDTVGQRDLIG
                     IFLGVTHIHIKKPPHVAGKRVYWSLIPLGTSVPGGGNALITATTAGFYMGDRANTFLS
                     TATFSPSTNFKGQFTLPLRTNIWSSRNAWNYSGDYRWTYYPQYIWGLGGNTAPSNKAL
                     IRYTYVRVYQNALKRISGKPYLFGGVGYDLDYHSNVRPANDTLDLARFAGYSHGTGGH
                     SSPVSSGITFNLLYDSRVNLLNPLPGWYYNVVLRVNPKFLGSDDLWYSLYGDARKYIS
                     FDHWHENVLAFWSYLWTTLGSNPPYLDLPAITWDAYQRSGRGFYPGRYMGKTLWDFEA
                     EYRRSITGDELLGFVVFANMNSVTEPGTREFAYLHAAAGAGLRVKFNKHSGTNLCVDL
                     AASKGYWALYFNLGEAF"
     gene            6996..7997
                     /locus_tag="VGQ51_15715"
     CDS             6996..7997
                     /locus_tag="VGQ51_15715"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEV9038082.1"
                     /translation="MATWAQTRSPDLPAKPAIQLLTSGTKTSLRGLSVVSDKVVWVSG
                     SNGQVGKSLDGGKTWSWITVPEYEKRDFRDIEAFDKNTAIIMAIAEPAVILKTTDGGK
                     SWRLVYQNETKGMFLDAMEFWNVKSGIVVGDPVNGRFFVARTFDGGDSWHEIPTRYMP
                     QADPGEGCFASSGTNVRNLDRDEAVFVSGGPSSRIFVRDSAITLPILQGTTSTGANSV
                     AVKDTRTLHGGFHLIVVGGDFAHDTIREKNCFLSNDRGKTWLSPTTPPHGYRSCVEFI
                     GGNNVLCCGTSGVDLSTDDGMNWQLISTTGFHACRLAKKGKTVFLAGSNGRIAKLIL"
     gene            complement(8006..11524)
                     /gene="smc"
                     /locus_tag="VGQ51_15720"
     CDS             complement(8006..11524)
                     /gene="smc"
                     /locus_tag="VGQ51_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV9038083.1"
                     /translation="MRLKSLEIKGFKSFADKTHISFDEGITGIIGPNGCGKSNIIDSI
                     RWVIGEQKISHLRSENLESLVFNGSKTRSASGLAEVSLTFENTKNLLPTEFSTVTVTR
                     KFYKSGESEYRLNDVSCRLKDIQNLFMDTGISTDSYAIIELGMVDDIIKDKDNSRRRM
                     LEQAAGVTIYKTRKKEARQKLDATEQDLSRIEDLLFEINNQLKSLESQAKKAEKYYEI
                     KKEYRELSIELAKAALEGFNLTYRELNEQSDTETDRKIRLEAVIATEEAGLEQERVGF
                     IEKERALQSMQHEFNDLLQRLRERENEKNLASQRLNFLKEKEDSLQEFLLKGEGQLKG
                     LEESIIFTRQQAVEEENKLSGLQEQLESLKDAVDESRSVFDARRVSVDALRRENAQIQ
                     RDQFEAEKKVAVADTSIQNLQRSIRQLEDERAIRNSQLKQLEEERMSKEQDLGSRQEA
                     LRQLQEHHERMKENILQAQGQLEVLRQRLADEGRTLDAKKNEHDLLKSLIDSMEGYPE
                     SIKFLHRNPSWREAPILSDIIYVKEEFRAAVENVLEPYLNYYVVNDLQEGMQAVHLLD
                     ANKKGKANFFLLDKIAGLSAAPEHDMEGMIPAMSVIEVDAHYRGLAEHLLGNVYIAES
                     EEALANSNGAVVLEKTGKYVKGKYSLTGGSVGLFEGKKIGRAKNLEKLQEEVRRLQSA
                     VDGLKLSIQEKHDEVLGYNEQLKEQAIRQTQDDINRLTNFVFSLENKIENLHSQQHTS
                     QSRLEELQLNLEETIAGIEDTRNAWAELVRIVGELKEKSQQAEEEYRSAEQAYSNANA
                     GYNEFNLQVTRQHSRINGLKQELEFKTRQLTELRDQIEVNASQLKQTHESIAESEEKL
                     GSSEAGLLELLRRKEEEEKKVNEADQAYYNLRNALSERESELRHKIKEKEQLEHLLSG
                     IKDRLNELKLQLAGMKERLNVEFRIQLDDIIDQPRKGEAPVEELQEKSDKMKKRLENL
                     GEVNPTAIEAFQEMKKRYEFILEQKNDLVSAKDSLLQTIQEVEATANQQFLDTFNRVR
                     ENFQRVFKALFTEEDTADLILENPENLAETGIDIVAKPKGKRPSSISQLSGGEKTLTA
                     TALLFSIYLIKPAPFCILDEVDAPLDDANVGKFTNMIRQFSENSQFIIVTHNKMTMSS
                     VDVIYGVTMQEPGVSKLVPVDFRGLN"
     gene            11733..12569
                     /locus_tag="VGQ51_15725"
     CDS             11733..12569
                     /locus_tag="VGQ51_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV9038084.1"
                     /translation="MNNFIVPIDFSETSKNAARYAAHISTLVQDAHLILYNVFDSIEY
                     GTDSSPLGTEGEEDASRKAIVEMALNSVKTELSGITSAGISCVAEESHRFLDTLEAYV
                     KAHDIQLIVMGLTGATRMEQVLMGSNALNIVRRAIAPVIIVPPDTHSQSAKNVLLLTD
                     FKDIENTIPIPSVKAVLDLFKPKLFIVNVDHEHYVQVTEEYKGERTKLEQQLKAYNPE
                     FYFIRLFDFVEAANQFVADYHIDLILTFPRKHSFLSNMFKTSSTKQLAYHSHVPIVAI
                     NA"
     gene            12625..13020
                     /locus_tag="VGQ51_15730"
     CDS             12625..13020
                     /locus_tag="VGQ51_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038085.1"
                     /translation="MGKVSPPLQAYFDQLCAIDPARRPDYIEQAHRLLKPGGKIAGLL
                     FDRELRRSVVPRSSSFVPFASLGNPGGPPFGGHRDEYERLFTRYFNLRTLAPCYNSIP
                     PRAGTELFFIAEKPQAPAGSNNENYPAPI"
     gene            complement(12956..14014)
                     /locus_tag="VGQ51_15735"
     CDS             complement(12956..14014)
                     /locus_tag="VGQ51_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEV9038086.1"
                     /translation="MRIEPTPGAAGYYFPAEFAPHSATWLSWPHKEASWPGKISSIYP
                     FYGQFVLELTKGEEVCINVADEAMQAFATRYLMEAGVDMERVRFFFHPTNDAWCRDHG
                     PAFLINPRAPERKVIVDWGYNAWGSKYPPYDLDDVIPSLVGKALGLPVFHPGIIMEGG
                     SVDFNGEGTLITSTCCLLNPNRNPGLDRHRIEGYLRDYYGVEQILWVGEGIVGDDTDG
                     HIDDTVRFVNGDTVVTVIEEDKRDENYQLLIDNLAELSAMRLLNGKQLNIVELPMPKA
                     VVYEDQRLPASYANFYIANEAVIVPTYRCDRDEKALLILQDCFPTRRVVGIDSTDIIW
                     GLGSFHCLSQQEPAVFPL"
     gene            complement(14004..14879)
                     /locus_tag="VGQ51_15740"
     CDS             complement(14004..14879)
                     /locus_tag="VGQ51_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEV9038087.1"
                     /translation="MSKVKIGLVQMSCTADKASNLQKAMARVREAAGKGAQIVCLQEL
                     FTSLYFCDVEDYGNFGLAEPIPGPSTEVLGRLAGELGVVIVASLFEKRTQGIYHNTTA
                     VLDADGSYLGKYRKMHIPDDPAYYEKFYFTPGDLGYKVFKTKFSTIGVLICWDQWYPE
                     AARITALMGAEVLFYPTAIGWATSQDEATNTEQYNAWQTIQRSHAVANGVHVVSVNRV
                     GPEQEGAMKFWGGSFVANPFGSLLYQASHDREETEVIEIDTAATDRYRTHWPFLRDRR
                     IDSYQTITKRYIDED"
     gene            complement(14994..16061)
                     /locus_tag="VGQ51_15745"
     CDS             complement(14994..16061)
                     /locus_tag="VGQ51_15745"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEV9038088.1"
                     /translation="MSLPKFSTATQSFHGELKRRISSYFEEVGKAQTGNYDLFLKAVI
                     LMAAFIFIYIHLVFFTPNAFWAIVESVLLGGVVAAIGFNVMHDGAHGSFSKYKWVNHV
                     AAFSLNILGGNSFMWNMKHNVVHHAYTNVEGIDDDIDIQPWMRMSEHQKRYRLHRYQH
                     LYFWALYCVLYIFWIFVLDYNKYFKSKIGAVPLKKMKLSDHFVFWGFKLFHAFLFIGL
                     PIYRLGWFDWMVSFIIFTMVAGFVLSMVFQLAHTVEHTAFPVADETTGKMEDEWAIHQ
                     IKTTANFATGNPLVSWLVGGLNFQIEHHLFPKISHVHYPAISKIIRQACKEYGLVYIE
                     YPRVRYAVASHVAFLRQMGRK"
     gene            complement(16199..16945)
                     /gene="bshB1"
                     /locus_tag="VGQ51_15750"
     CDS             complement(16199..16945)
                     /gene="bshB1"
                     /locus_tag="VGQ51_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEV9038089.1"
                     /translation="MKSTKLDILAIAVHPDDVELGCAGTLLMEKRNGKKTGVIDLTRG
                     ELGTRGTAELRGQEAARSAAILQLDVRENLGMADGFFRNDEEHQRLLIRAIRKYRPEI
                     VLANALEDRHPDHGRAGHLIVESCFYSGLRKVETTGEAGRPQEHWRPKYVFHYIQDRY
                     RDPDFVFDITPVFDTKVQSIEAYSSQFFSTTYGENEPQTYISSPEFLQSLIGRHQQFG
                     KMIGVAYAEGFTTQKMIGIRDFGALVQIDT"
     gene            17019..18422
                     /gene="lpdA"
                     /locus_tag="VGQ51_15755"
     CDS             17019..18422
                     /gene="lpdA"
                     /locus_tag="VGQ51_15755"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV9038090.1"
                     /translation="MPYDVVVLGSGTGGYPAAIRASQLGFKVAIIEKENLGGVCLNWG
                     CIPTKALLKSAQVYQDILHAKEFGIDATGTADFPAVIKRSRGVADKMSKGVQFLMRKN
                     KIDVVTGYGKLKAKGQIEVTGADGKTQIVEGKHIIVATGARSRELPNLKQDGKKVIGY
                     REAMTLPAQPKSIIVVGSGAIGVEFAYFYYSMGTKVTIVEFLPRIVPVEDEEISKELE
                     KTYKKYGIEVLTSSEVTSLDTGGNGVKAKVKTPTGEVTLEADIVLSAVGIAANIENIG
                     LETMGIKTEKGKIVVDKYYQTSVPGIYSVGDCVPGQALAHVSSKEGIICVENIAWKEK
                     KYAHQPETLDYNNIPGCTYCTPEIASVGLTEKQARDAGYEVKVGKFPLTASGKATGVG
                     HNEGFVKVVFDAKYGEWLGTHAIGYNVTEIITETVLGRKLETTYHEVLDAVHPHPTIS
                     EAVKDAVEAAYGEAIHL"
     gene            18481..18678
                     /locus_tag="VGQ51_15760"
     CDS             18481..18678
                     /locus_tag="VGQ51_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038091.1"
                     /translation="MDSRNSSIIYFILGLYTLMISWHYNHNWILLLIHWIIWPVYLVY
                     ELLIGHLSHDLWRTIPLGYFK"
     gene            complement(18746..19426)
                     /locus_tag="VGQ51_15765"
     CDS             complement(18746..19426)
                     /locus_tag="VGQ51_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV9038092.1"
                     /translation="MKLTFYGHATFSVTVKGKTLLFDPFFNGNPALKNIDIDKVKADY
                     LFLTHGHGDHTADAVSVAKRTGATCVAAAEIAGWLGKQGLEKVHPINHGGPVPFEFGK
                     VRAVNAIHSSSFSDGSYAGNPLGFVFNTTEGDFYIAGDTALTMDMQLIPLWAKLQFAV
                     MPIGGNYTMDVADAIHAADFVKCATVVGVHYNTWPIIAIDAEKAKADFAAAGKKLLLP
                     ASGETIEV"
     gene            19511..20068
                     /locus_tag="VGQ51_15770"
     CDS             19511..20068
                     /locus_tag="VGQ51_15770"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV9038093.1"
                     /translation="MQHNLSKKSILVAITLLVTGLSFRAIAQPITGDEIKTQFVKDWE
                     RAKSYTLDYLNTMPPDKYSFKAVDSIRSFAQQMLHLASGNVGLISQATGEKTDFPRNL
                     EKSPTAQNKDSVVWYVTRSYDFAIESIQKLDPNKYGEMLGQDPRKVSRFAWLLKAFEH
                     QTHHRGQTTIYIRLQGIRPPNERLF"
     gene            complement(20171..21241)
                     /locus_tag="VGQ51_15775"
     CDS             complement(20171..21241)
                     /locus_tag="VGQ51_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEV9038094.1"
                     /translation="MSKLFTPLQIRDIILGNRIVVSPMCQYSSHDGKAEDWHLVHLGS
                     RAVGGAGLVFTEATAVSAEGRISPFDLGIWKDEHIEGLRRIVKFVRSQGSEIGIQLAH
                     AGRKASTKEPWNGGGLVKESEGGWRVLGPSAVAYSERYGLPVEMTVADIRRVVDEFGA
                     AARRAMLAGFKVIELHAAHGYLLHQFLSPLSNRRSDEYGGVFGNRVRLVMEVVDAVRG
                     VWPVVYPLFVRISATDWADGGWSPEDAVRLAALLKDRGVDVIDCSSGGLVPYQQIPAG
                     PGYQVEFSRQIRAATGILTAAVGMITGTMQAETVLVTGQADLVVMARQILRDPYFPLH
                     AANELGYADIHWPLQYERARPK"
     gene            21286..22092
                     /gene="modA"
                     /locus_tag="VGQ51_15780"
     CDS             21286..22092
                     /gene="modA"
                     /locus_tag="VGQ51_15780"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV9038095.1"
                     /translation="MYHVRWVLPAICAATTFVTAYKPLPASAPAETCQSSTEVLVAAA
                     ADLRFAMDSLVAAFSLENPDITVKPTYGSSGNFFQQIQNDAPFDLFFSADVDYPRQLK
                     EKNLTLSDIYIYGTGQLVLWSKTLDPNKKQMNTLLDKSVTKIAIANPAHAPYGKRAEE
                     SLHHYNLYDKVKDKLIFGENIAQTAQYAQSGAAEVGIIALSLAVSPSMRQTGGKFWLI
                     PAKAHQPLLQGFVLLPHSKGNAGAEKFARFCNSHTAKDVLHNFGFTQPSS"
     gene            22106..22777
                     /gene="modB"
                     /locus_tag="VGQ51_15785"
     CDS             22106..22777
                     /gene="modB"
                     /locus_tag="VGQ51_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEV9038096.1"
                     /translation="MIDWLPIWLSLRLALVTTAILFVLGIPLSYRIAYSRRRWSIFAE
                     ACISLPMVLPPTVLGFYLLLAFSPAGSFGHFLDHYLDLRLVFTFRGLVVASLIYSLPF
                     MVNPLLAGFKNLPPHLREASRTLGKSDWTTLRRILLPNMRPSLVTGAILSFAHTIGEF
                     GVVMMIGGAIPGRTRVASVAIYDEVQSLNYPLANHYAIVLLLFSFSILLVVYAINHRQ
                     SKAVL"
     gene            22774..23781
                     /locus_tag="VGQ51_15790"
     CDS             22774..23781
                     /locus_tag="VGQ51_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9038097.1"
                     /translation="MSSPPANLIFRATKMLHTAQGPQLFDVSFDLPQGRLLAVYGSSG
                     SGKTTLLRILAGLTETASGHIELDGEIWLNHPNNPNVPSNLNVPSNLNHPNSPNHPNN
                     PNDPHGSNRPPGPGRYTSLPTRHRSIGFVFQDFALFPHLTVRGQLEFALPKHADRSII
                     DELLTLMELEELQHRRPALLSGGQQQRIAIARAIARRPRLLLLDEPLSALDDEMRSRL
                     QNYIGIAHRHYKLTTILVSHYLPEIISLSDEAIILEGGRILDRGIPETLFPKDRIIGD
                     IRLNGEIIDITNAGDTSIIHVLSAGSTIKIVTPSGANMHIGQHVTIAYHHFNPVILPA
                     T"
     gene            23817..>24471
                     /locus_tag="VGQ51_15795"
     CDS             23817..>24471
                     /locus_tag="VGQ51_15795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9038098.1"
                     /translation="MPSLSVWEKESFFAPADVIIAGSGLVGLWSAYYLKKRAPSLTIT
                     ILERGIIPTGASTRNAGFACFGSLTELIKDLRSSGEDRMLQLVEMRYKGLKKIKKTLG
                     GKAIGWEKYGGYELIAEGDADEIRSQIDLLNHLLKKVTGQQKTFRLQNDLISRFGFSG
                     TQLLIGNPSEAQLHSGRLCQALLCLVQSMGVTVLNNIEITGFEKTGGQLLLHTSLPFP
                     "
BASE COUNT         5768 a         7734 c         5513 g         5456 t
ORIGIN      
        1 tcgaagataa cgggcgaata caatgtcaac gtggccacaa gaagcaggca ttatagtggg
       61 gagccgaccg gagacgtcca tcccgccatg ctggctatca ccgcggatat gtattccctc
      121 caaaacacga tcctgcctac aggatatacc ttcagtatcg tggacgcaaa aggcgatata
      181 cttttcgatg ccagaccggg cagggcgctc ctgtcgaata tttttgtgga ggcgggcgac
      241 aacacggacc tccaacaatg cgtgcgctac cggtatgagc gcttttttcc gaatatcacc
      301 cttcggggaa gggaagtggc attgctaagc aaacccatgg ccggcacaaa ctattcttta
      361 ctggtatact acaatctttg ggcaatgcag gaagttcatt tccattctct cggtctttcc
      421 tgtttttgca tgtcctgtat ccttttgctg atcgccctgg tcgcttttat caatgaatgg
      481 ggcaagaaaa ggccttcatt gcttcgcctt tccaaggtca atttcgactg gctgcgcccg
      541 gttccggcta agtacggcta ctaccggcac ctgattatct ggctgcggat attcctcgcg
      601 ctcttcctgg tggcatgggt gttcatcgaa atgctgctgc ccgaatatga acccgcactt
      661 ttgctgatct cgctgtcctt ccccatcttt atagccgttc attattttgt cctgcgcgaa
      721 aaatattatc atattgagcg aggcgaagcg atcatcctgc cccctgcact ggcacccctg
      781 accgttgctc tgctggcaat ctttatctat gtctgcaccg cggggctggg tgccggcgtc
      841 atcgtgacca ccctcgtggt cctggtttta ttcctgggta cgatcctgga gtcctggcgg
      901 agattcgggc gtacgatcgc caaccacggc gtgaaagcct tgctggaaaa atatacttgg
      961 gctatactgc acggcgtttt tttgattacc ctggtacccg ccatagcctt gttcatcctt
     1021 ttctataaag aagaacacaa cggcatcacg cggatgggga tgctgagcat gatgcagaag
     1081 atcgatgaca gggcccggac tatcgacagc aaaaaaaatc tgtatctccg ggacgacaac
     1141 cccgatttca tgcgggcctc caaattcagg aacggcattt atttcaccga ccggtctgca
     1201 gacactcccg ccctgttttc cgaacccgtc aaaacgaatt tcacggctta cagtacactt
     1261 cacaatttat tgttccaacc cgacagtacc tctctcgaag ctctggcccg gctgaatgca
     1321 gcctcggacg gctcctggca attcgcaaca gtcggacatc cgaaacgact cgtcctcgat
     1381 cattgcaacc ccgtgggtag ttgcaaaaat gccttttcac tgtcctcgga ccccgtaacc
     1441 ctgtatccgg ccctccgttt cttcctatac aggtttttgt cgctcggcgc aatgcgtatc
     1501 ttcctgctgt tgcttggcat ctttgcgctg atgttgatcg cccaacgcct taccgcttcc
     1561 ctcgccagcc acgtatttct cctccggttg ctggaaaaat acggccgtgc caattatgac
     1621 gaagccaggc tccccgaggt cctctatccc cccgtcgaag aaaacaccac cttacatatg
     1681 caggtccgcc tcgagaaacc ctatgacgat ctttggaaga agctgaccac cgaggaaaaa
     1741 tacgtcctgt acgatttcgc gctggacggc ttcaccaact ataaagcggg aatactgatc
     1801 tacaaactgc tccatcgcga tcttctctac gtcgacagaa acttcctgct ccgcatccgg
     1861 tccgctgatt ttcataacta cctgctaagt aaagacatct tcaatccata ccaggagaac
     1921 aaggaagaca taaaagtgta caaatacatg atgagcgtca gaaagcaagg tttctggcag
     1981 gccttccgcg taccgttgca ggtgatcctc ggagcggcag gtctgttcat cttcttcaca
     2041 caggaagcgc ttagccaaaa gataatcggg ctgcttacct cgctgccatt cattactcaa
     2101 atgctggcgt cttttttcga aagatcgaac ggtcaaaaaa acaaggatcc cggctcagac
     2161 caaaatcccg atctttgatc catgcatttt accgacccgc tccccaatcc cgacgagtcc
     2221 aacaagccct cccggaaaaa acccattttc cccgtgaacg agggcctgcg cagctatctg
     2281 aaacagcatg ggcgcgaagt acggttgccg gtatcctatc aggacctgct tcttcatatc
     2341 acctattccg tccccctgaa agataagaac cagcaggaca cactctggga aaccgccctc
     2401 tatgacatgc gtcattggga attcatccgc gaaggcctgg tccagatgta cgctatcctc
     2461 aagacggaag gcgatctcac cttcaccaaa cacctcgacg tcgcccgcat cgatttctgc
     2521 agcttcgcca actctcatcc cttccgtatc cgcatcgtca ataaattcaa cgacaactac
     2581 gatcactact acatcaaaca ggcggacgcc tcccgcatct acggtctgga actggaacac
     2641 cttctctccc ccaaccgcat cacttttttc acgcacgaca ataccctcgt cgaagagcat
     2701 atcccgggca tccccggcga tctcttcata cgcaacttcc tggaagacac acaaaccaac
     2761 aagatccgcc tttcgaaaga attcgtcaaa ttcaacgaac ggtgtttcgt ccggctcctc
     2821 ggtgacatgc gcgcctataa cttcgtggtc gatatcacgc cggatatcga agattaccaa
     2881 taccgtatca gggccatcga tttcgatcag caatcctatg aaggccgcaa aaatctctat
     2941 atcccccaat tcttcaagga gaaccggccg ttcgtcgagc tcgcactcca gcacctcaac
     3001 aaggattcta ttgaacagta ccagacggaa gaaaggacga tgatggcctt ccgcgtggcc
     3061 tcttcccgat tccggttgat ggatctgctc aatatcatgt cccgcgacaa gcccctgtcc
     3121 agccccgaaa agctcctcga gcttaagacg ggcctggccg aatttttcgg gaaccccgcc
     3181 ttcctgaaat gccgctccat cggtcaggta gtcaagcgac agctcaaaca aaccttgcag
     3241 aagaatcttt cgcttctcca aaaaaatctt ggcaaattcg aagattagcc cgctatgcct
     3301 tatcttcgcg gcgccacggg acccctggtc caacctgcgc ggcgcaaagt ttcaatatgg
     3361 ccaaatacag atctggtgtt cttttcggcg acgaactcga agcactttac aaagacgcaa
     3421 aagataatca attcgcactt ccggcggtca acacgatcgg tacgaatacc attaacgcta
     3481 ccctcgagac ggcggcaaag gtcaattccc ctgtgattat ccagttctcc aacggcggcg
     3541 cccagttcat cgccggaaag ggaatgccca acgataagct ccagggcaat atctccggtg
     3601 gcgtttccgg agccctccac atccataatg tcgccaaata ctatggcgta cccgtagtgc
     3661 tccataccga tcacgcatcc aaaaaatggc tcccctggat cagcggactc atcgacgccg
     3721 gcgaacaatt cttcagggaa aagaaacaac ctctcttcag ctcgcatatg ctcgatctgt
     3781 ccgaagaacc catcgaggaa aatatccata cctccgtcga ttttttccgg cgtatgaagc
     3841 ccctcggcat ggccatcgag atcgagctgg gcgtcacggg aggtgaagag gacggagtcg
     3901 acaatagcgg cgtagagaac gagaaactgt atacccagcc ccagcatgta gcctatgcct
     3961 acaaagagct cagcaagatc gggcccctct tcagcgtggc cgcggccttc ggcaatgtac
     4021 acggcgttta tagccccgga aatgtcgagc tccggcccgt catcctcaag aacagccagg
     4081 actatatcga aaaggaattg aagaccggac ccaaaccagt ctacttcgtt ttccacggcg
     4141 gcagcggaag tccccagcac cagatacgcg aagccatcgg ctacggcgcg atcaaaatga
     4201 acatcgacac cgatctccaa tgggcctact gggaaggcgt gctgcagttc tacaaaaaga
     4261 atgaagccta tctccaggga cagctcggca accccgaagg atcggacaaa cccaacaaaa
     4321 aatactacga cccacgcgta tggcaacgca aggcagagga gaatttcgtg aagcgtctcg
     4381 aggtcgcctt ccaggacctc aattgcatca accgcaacgc gtgacgcgcg gtagcgcccc
     4441 cgacaacgcc tgacgcacgg tagcgccccc ccgcaacgcc tgacgccacc cgacggccgt
     4501 cgattttttc ttttcccgca ggaaggttaa attgcagtat aatatctaac cgcaatccca
     4561 tgccttccaa aaaaattctc gacgcgctcg gcgacaaagc atccttctta ctcgatcata
     4621 agcccgtcct cgacaaatcc gtcctcagcg ccttcccttc ccccgaacat gtcgaagaaa
     4681 tatgggtcaa ctccgaccgc aacaaccagg tcctgagaag catacaggtc ctcattggcc
     4741 atggaagact cgcggatacc ggctatctct ctatctttcc cgtcgaccag ggtgtcgaac
     4801 acagcggcgg cgcatccttt gcggcaaacc cgatgtattt cgaccccgaa aacatcatcc
     4861 gcctggccat cgaagccggg tgcagcgccg tcgcttccac cttcggcgtc ctcagcatca
     4921 tgagtcgcaa atatgcccac cggataccct ttctcgtgaa actcaaccac aacgaattcc
     4981 tcagcctgcc taaccgctac gatcaaacga tgtatggcac agtaaagcat gcctggaata
     5041 tgggagctgt cgccgtcggc gcaaccgtct attgggggag tgccgagagc gatcgccagc
     5101 tcaaagaggt cgccgaagcc ttcgaacagg cgcacgagct gggcatggct accgtcctct
     5161 ggtgctatac ccgcaacccg ggcttcaaag tggacggtgt agattatcat acctccgccg
     5221 acttcaccgg ccaggccaac catctcggcg taacactaag agccgacatc atcaaacaga
     5281 agctgccgac caacaacggt ggctatctcg ccaccaaaca cggcaagacc tctccgctcg
     5341 tatattccaa attaacgcac cctgacaacc atcccatcga tctcacccgc taccaggtgc
     5401 tcaactgcta cagcggtcgc gtgggaatga tcaacagtgg cggtgaaagc aaaggcgcct
     5461 cagacactgc cgatgcagtc ttcaccgccg tcgtcaacaa acgcgccggc ggcatgggca
     5521 tgatcatggg ccgcaaggcg ttccagcgtc ccttcaaaga aggcgtcgat atcatccaga
     5581 tggtacagga tgtctatctc gacaaggaca ttacgatcgc ctaaacccgc gcgtccccgc
     5641 cgccccgcta aaaggcctca cccaaattaa aatacaacgc ccaatacccc ttgctcgccg
     5701 caagatctac gcaaagattc gtgcctgagt gcttattgaa tttgacgcgc aatcccgcac
     5761 ccgccgccgc atgcagatac gcaaactcac gcgtccccgg ctccgtcacc gaattcatat
     5821 tcgcaaagac cacgaaaccc agcagctcat cacccgtgat cgaccgccgg tactccgcct
     5881 cgaaatccca aagcgtcttc cccatatacc ttcccggata aaacccacgc cccgaccgct
     5941 gataggcatc ccaggttatc gccggcagat cgagataagg tggattgcta cccagcgtcg
     6001 tccacaaata agaccagaac gccagcacat tctcatgcca gtggtcgaag ctgatatatt
     6061 tgcgtgcatc accatacaac gaataccaaa gatcgtcgct gcccagaaat ttcgggttga
     6121 cacgcaagac cacattgtaa taccaccccg gcaacggatt caacagattg accctgctat
     6181 cgtacaaaag attgaacgta atccccgacg acaccgggct ggaatgcccc ccggtcccat
     6241 gcgaataccc cgcgaatctt gccagatcca acgtatcgtt cgcgggccgg acattgctgt
     6301 gataatccag gtcatacccg acgcccccaa aaagataggg cttcccgctg atccgcttca
     6361 gcgcattctg gtagacccgg acataggtat atctgatcaa cgccttgtta ctcggcgccg
     6421 tattcccccc caacccccag atgtattggg gatagtaagt ccaccggtaa tccccgctat
     6481 aattccaggc attgcgcgaa gaccagatat tcgtacgcag cggcagcgta aactgtccct
     6541 tgaaattggt gctgggtgaa aaagtggcgg tagacagaaa agtattggcc cggtctccca
     6601 tataaaagcc ggcagtcgtc gccgtgatca gggcattccc gccgcctgga acggaggtgc
     6661 ccaggggtat caatgaccaa tacaccctct ttccagctac gtgcggcggc tttttgatat
     6721 ggatatgcgt gacccccaaa aaaatgccga tcagatcccg ctggcccacg gtgtcattca
     6781 ccaacttgtc cggcggcaca agggtcccgg tcgggttttg ggcggacaga cgaatgatcc
     6841 aacaacagca aaggacgacg gcaaaaaatt taggcatata tccgtgaata ccaatattgt
     6901 gctacatttc aggtatgaac cgcttccccg atcctccccc ggcaatacgg cttttttggt
     6961 cattctgttg ctttttcgct acaacggcct tgcccatggc cacatgggcc cagacccggt
     7021 cgcccgatct cccggcgaag ccggcgatcc aactactgac cagcggcact aaaacttcgc
     7081 tgcgcggcct cagcgtcgtc tccgacaaag tcgtctgggt cagcggcagc aacgggcagg
     7141 taggcaagtc cctcgacggc ggcaagacct ggtcttggat caccgtcccc gaatatgaaa
     7201 aacgcgactt tcgcgatatc gaagctttcg ataagaatac cgccatcatc atggccatcg
     7261 ccgaacccgc tgtaatcctg aaaacgactg acggcggcaa aagctggcgg ctggtttacc
     7321 agaacgagac caaaggcatg ttcctcgatg ctatggaatt ctggaatgtg aaaagcggga
     7381 tcgtggtcgg tgaccccgtt aatggccgat tcttcgtagc ccgcaccttc gatggcggcg
     7441 attcctggca cgagataccg acgcgatata tgccgcaggc cgatcctgga gaaggttgtt
     7501 tcgccagcag cggcaccaat gtccgtaatc tcgaccgcga cgaagccgta ttcgtcagcg
     7561 gcggccccag ttcccgtatc ttcgtccgcg actccgccat caccctcccc atcctgcagg
     7621 gaaccacatc caccggagca aactccgttg cggtgaaaga tacccgtacg ctccacggag
     7681 gtttccacct catcgtcgta ggtggcgact ttgcacacga taccatccgc gaaaaaaatt
     7741 gtttcctctc caatgatcgt ggtaagacct ggctctcccc cacgacccct ccccatggct
     7801 atcgcagttg cgtcgaattc atcggcggca ataacgtcct ttgctgcggc acttcaggcg
     7861 tcgacctttc caccgatgac ggaatgaact ggcagctgat ctccaccacc ggcttccacg
     7921 cctgccgcct cgcaaaaaag ggcaaaaccg tcttcctcgc cggttccaac ggccgtatcg
     7981 caaaattgat cctctaaaag ttagattaat tcaaccccct gaaatccacc ggcaccagct
     8041 tactgacccc cggctcctgc atcgtcaccc catagatcac atccacagaa ctcatcgtca
     8101 tcttattatg cgtaacgatg atgaactgcg aattctcact gaactggcgg atcatattgg
     8161 tgaactttcc gacattggcg tcatccagcg gcgcatccac ctcatccagg atacaaaatg
     8221 gagccggctt gatcaggtat atggagaaca atagcgccgt tgccgtcagc gtcttctcac
     8281 cgccgctcaa ttgactgatg gaggacggcc gcttcccttt cggcttggct acgatatcga
     8341 tgccggtctc tgcaagattc tcgggatttt ccaggatcag gtctgccgta tcctcctcgg
     8401 tgaacaaagc cttaaacacc cgctgaaagt tttcacgtac ccggttgaag gtatccagga
     8461 attgctgatt ggccgtagcc tccacttcct gtatcgtctg cagtaggcta tccttcgcgc
     8521 tgaccagatc attcttttgt tctaaaataa actcataccg cttcttcatc tcctggaagg
     8581 cctcgatagc cgtcgggttc acttcgccaa ggttctcgag acgcttcttc atcttatccg
     8641 acttctcctg caactcttcc acaggggcct cgcccttcct cggctgatcg atgatatcgt
     8701 ccagctggat cctgaactct acgttcagcc gttctttcat cccggccagc tgcagcttca
     8761 gctcgttcaa ccgatccttg attccactga gcaaatgctc gagctgttct ttttccttga
     8821 tcttgtgccg caattcgctt tcccgttccg acagcgcatt ccgcaggttg taataggcct
     8881 ggtccgcctc attcaccttc ttctcctcct cttccttgcg acgcaacagc tccagcaaac
     8941 cggcttccga actacccaac ttctcttcac tttccgcgat actctcatgc gtttgcttca
     9001 gttgcgaggc gttcacctcg atctggtcgc gcaattcggt caattgtctc gtcttgaact
     9061 cgagctcctg tttcaaaccg ttgatccggc tatgctgacg cgtcacctgc aggttgaatt
     9121 cattatatcc ggcattggca ttactatagg cctgctcggc ggaccggtat tcctcttccg
     9181 cctgttgtga tttttccttc agctcgccga cgatcctgac cagctcggcc cacgcattcc
     9241 gcgtatcctc gatgcccgcg atggtctcct ccaggttgag ctgcaattcc tccagccgac
     9301 tttgtgacgt atgctgctga gaatgcagat tctcgatctt attctccaac gaaaaaacga
     9361 aattcgtaag ccggttgata tcgtcctgcg tctgccggat cgcctgttct ttgagttgct
     9421 cattatagcc caggacttca tcgtgcttct cctggatcga aagcttcaga ccgtccacag
     9481 ccgactgcag ccgccggacc tcctcctgca atttttcgag attcttggcg cgcccgatct
     9541 tcttcccttc gaacagaccc acactgcctc ccgtaagcga atatttccct tttacatatt
     9601 tcccggtctt ttcgagcact acagcgccat tgctgttggc cagcgcttcc tcactctccg
     9661 cgatatagac atttcccagc aggtgttcgg ccagcccccg gtaatgggca tccacttcta
     9721 ttacgctcat cgcagggatc atgccttcca tatcatgctc tggcgccgcg gacaatcccg
     9781 cgatcttatc cagcagaaag aaattggcct tccctttctt attcgcatcc agcaggtgaa
     9841 cggcctgcat tccttcctgc agatcgttca ctacatagta gttcagatag ggctccagta
     9901 cgttttccac cgccgccctg aactcttcct tcacatagat gatatccgac aggatcggtg
     9961 cctcgcgcca ggacggattc cgatgcagga acttgatgct ctcgggataa ccttccatcg
    10021 agtcgataag gctcttcagc agatcgtgct cattcttctt ggcgtcgagg gtacggcctt
    10081 catcggccag ccgctgacgc aaaacctcca gctgcccctg cgcctgcagg atattttcct
    10141 tcatccgctc gtgatgctcc tgcagctgcc gcaaagcctc ctgccggctc ccgagatcct
    10201 gttccttcga catccgctcc tcttccagct gcttaagctg gctgttacgg atagcccgct
    10261 catcctccag ttgcctgatg cttctttgca agttttgtat cgacgtgtcc gccaccgcaa
    10321 ccttcttctc cgcctcgaac tgatcgcgct ggatctgggc gttctcccgg cgcagcgcat
    10381 ccacgctcac acgccgcgca tcgaaaaccg accgggattc gtcgacggcg tccttcagcg
    10441 attccagctg ttcctgcaga cccgataatt tgttctcttc ttccaccgcc tgctgccgtg
    10501 taaagatgat gctttcctcc aaccccttca gctgcccttc ccccttcagc aggaattcct
    10561 gcaagctgtc ctctttctcc ttcaaaaaat tcaagcgctg cgaagccagg ttcttctcat
    10621 tctcacgctc gcgcaaccgc tgcagcagat cattgaattc atgttgcatg gattgcaacg
    10681 cccgttcctt ttcgatgaac cccactcttt cctgctccaa tcccgcttcc tccgtggcga
    10741 tcacggcctc caaacggatc ttccggtccg tctcggtatc ggactgctca ttcagctcgc
    10801 gataggtcag gttgaaacct tccaatgcag ccttcgccag ctcgatgctc agctcgcggt
    10861 attctttttt gatctcataa tacttctccg cctttttcgc ctgactctcg agactcttca
    10921 gctgattatt gatctcgaat aacaggtctt cgatccggga caggtcctgc tctgtcgcgt
    10981 ccagcttctg cctggcctct tttttccgcg ttttatagat ggtgaccccc gcggcctgct
    11041 cgagcatcct tcgccggctg ttatctttat ctttgataat gtcgtcgacc atgcccagct
    11101 cgataatagc ataactgtcg gtgctgatcc ccgtatccat gaagagattc tggatatcct
    11161 ttagccggca ggaaacgtca ttcaatcgat actcgctttc tccgctctta taaaatttac
    11221 gggtgaccgt caccgtgctg aattccgtgg gaagcaggtt cttggtattt tcgaaggtca
    11281 ggctcacctc ggcaagaccg gatgcgctgc gcgtcttgct tccgttgaat accaggctct
    11341 ccaggttctc cgaccgaaga tgactgatct tctgctcgcc gatcacccac ctgatactat
    11401 cgatgatatt actcttacca caaccattcg ggccgatgat gcccgttatg ccttcgtcga
    11461 agctgatatg tgtcttatcg gcaaaactct tgaatccttt aatttcgagg ctctttaatc
    11521 gcacagtact tatattttga atttctcatt ccctctcccg ccgcacttcc cgcaactatc
    11581 ttcttccata tcccaacatc atccgcgggg gagccggcaa acgtacagta aaatacctaa
    11641 atcttcccga aattcgttcc ccctcgccgc cacctgttga aaactcgtta ataacaattt
    11701 catcgtatat ttagcccaaa ctagctacgt atatgaataa ttttattgtc cccatcgact
    11761 tctcggaaac gtccaaaaat gccgcccgtt acgccgcgca tatctccacc ctggtacagg
    11821 acgcgcatct tatcctgtac aatgtttttg actccataga gtacggtacg gacagcagcc
    11881 cgctgggcac ggaaggagaa gaggatgcca gcaggaaagc gatcgtcgag atggcgctga
    11941 acagcgtaaa aacggaactc tcgggcatca cgagtgccgg catttcctgc gtggcggaag
    12001 aatcccaccg tttcctggat acccttgaag cgtatgtcaa agcgcacgat atccagctca
    12061 ttgtcatggg ccttaccggg gcaacgagaa tggaacaggt gctcatggga agcaacgcgc
    12121 tcaacatcgt cagaagggcg atagcgccgg tgatcatcgt gcccccggat acacattccc
    12181 aatctgccaa aaacgtcctc ctgctcaccg acttcaagga tatcgagaat accatcccca
    12241 tcccgtcggt caaggcggtg ctcgacctct tcaagcccaa gctgttcatc gtcaacgtcg
    12301 accacgaaca ctatgtccag gttaccgaag aatacaaagg ggagaggacc aaactcgaac
    12361 aacagctgaa ggcttacaac cccgaattct atttcatccg tttgttcgat ttcgtggagg
    12421 ccgccaacca gttcgtcgcc gactatcaca tcgatctgat actgaccttc ccgagaaaac
    12481 attcattcct ctcgaatatg ttcaagacga gcagcactaa acagctggcc tatcacagcc
    12541 acgtacccat cgtcgcgatc aatgcctgat aaaacctccg gatataggga cgagcgttgc
    12601 ctgcaggggc acactggttg ggacatggga aaggtctcac cgcccctgca ggcttatttt
    12661 gaccagctct gcgccatcga cccggcaaga cgacccgact acatcgaaca ggcccatcgc
    12721 ctgctaaaac ccggcgggaa gatcgccggt ttgctcttcg atcgcgaact tcgtcggagc
    12781 gtcgtccctc gctcctcctc gttcgttccc ttcgcttcac tcgggaatcc gggagggccg
    12841 cctttcggcg gacatcgcga cgaatacgag cgtctgttca cccgatattt caacctcagg
    12901 acgctcgcgc cctgctataa ttccatcccg ccccgcgcgg ggaccgaact gttcttcata
    12961 gcggaaaaac cgcaggctcc tgctggctca aacaatgaaa actacccagc ccccatataa
    13021 tatccgtcga atcaatcccc accaccctcc gggtaggaaa acaatcctgc aggataagta
    13081 acgccttctc atcacggtcg cagcgataag tcggaacgat cacggcttca ttggcaatat
    13141 agaaattggc ataggacgcc ggaagccgct gatcctcata aacgacagcc ttaggcatcg
    13201 gcagctcgac gatattcagc tgcttgccat tcagcagacg cattgccgac aattccgcca
    13261 gattatctat caacagctga taattctcat cccgtttatc ctcctcgatc accgtaacta
    13321 ccgtgtcccc gttcacgaac cttaccgtgt cgtcgatatg cccgtccgtg tcatccccca
    13381 cgatcccttc gcccacccac agtatctgct ccactccata ataatcgcgc aaataacctt
    13441 ctatccggtg ccggtccaaa cctggattcc ggttgggatt gagcagacag cacgtcgaag
    13501 tgatcagcgt cccttccccg ttgaaatcca ccgagccgcc ctccatgatg atacccgggt
    13561 gaaagacggg taaacccaac gccttcccca ccaacgaagg gatcacatca tcgaggtcgt
    13621 atggaggata tttgctaccc caggcattgt atccccagtc cacgataacc tttcgctccg
    13681 gcgccctcgg gtttatgaga aaagcaggtc cgtgatccct gcaccatgcg tcattggtag
    13741 gatgaaaaaa gaacctcaca cgctccatat cgacccctgc ctccatcaga taacgggtgg
    13801 caaaagcctg catggcctca tcggccacat tgatacatac ctcctcccct ttggtaagtt
    13861 ccaatacaaa ttgaccatag aagggataga tcgacgagat cttgccgggc cagctggctt
    13921 ccttatgcgg ccagctaagc caggttgcgc tatgaggggc aaattctgcc ggaaaataat
    13981 aacctgccgc gcccggagtg ggctcaatcc tcatcgatat aacgtttggt aatggtttga
    14041 taggagtcta tccgccgatc ccgcagaaaa ggccagtgcg tgcgataccg gtccgttgcc
    14101 gcagtatcga tctcgatcac ctcggtctcc tccctgtcat gcgaagcctg gtaaagaaga
    14161 cttccgaacg gattggctac gaagcttcca ccccagaatt tcatcgcacc ttcctgctcg
    14221 ggacccaccc ggttgacgct caccacatga acgccatttg caacggcatg gctccgctga
    14281 atggtttgcc aggcattgta ttgctcggta ttcgtcgctt cgtcctggct ggtggcccaa
    14341 ccgatagccg tgggatagaa taagacctcc gcacccatca acgccgtgat cctcgccgct
    14401 tccggatacc attggtccca gcagatcaat accccgatag tggagaactt cgtcttaaaa
    14461 accttatacc cgagatcacc gggcgtaaaa taaaatttct cgtaataagc cggatcgtcc
    14521 ggaatatgca ttttccggta ttttcccaga tagctgccgt cggcatccag cacggcagtg
    14581 gtattgtgat aaatgccctg ggtacgtttt tcgaataagg aggcaacgat gacgaccccc
    14641 agctccccgg ccagccggcc caggacttcg gtggaaggac cagggatcgg ctcggcaagc
    14701 ccgaaattcc cataatcttc cacatcacaa aaataaagag aagtgaacag ctcctggaga
    14761 caaacgatct gcgctccctt gccggccgct tccctgaccc ttgccatagc cttctgcaga
    14821 ttggacgcct tatcggcagt acaactcatc tgcaccaggc caatttttac tttgctcatg
    14881 gccggcaaaa ttacagtatt ttaagggatt ccggagtgcc gccccattcg cgccttcctt
    14941 ccggcgcccc ggcccgctca gcgtccgccc cattcacgca accgccgcgt agactacttc
    15001 cgccccatct gcctcagaaa ggcgacatgc gaagcgacgg cataccgcac ccgcgggtat
    15061 tcgatataga ccaacccata ttccttgcag gcctgccgga tgatcttgct gatagcggga
    15121 taatggacat gcgatatctt ggggaaaaga tgatgctcga tctggaagtt caaccctccg
    15181 accagccatg ataccagcgg attgccggta gcgaaattgg ccgtcgtttt gatctggtgt
    15241 atggcccact catcctccat tttaccggtg gtctcatcgg ccacggggaa ggcggtatgc
    15301 tccacggtat gcgccagctg aaagaccatg ctcagcacaa aaccggcgac catggtaaat
    15361 atgatgaagc tgaccatcca gtcgaaccac ccaagccggt atatcggcaa cccgatgaac
    15421 aggaaagcat gaaacagctt gaaaccccag aaaacgaagt gatcgctcag cttcattttc
    15481 ttcaatggca cggcgccgat cttgcttttg aaatacttgt tatagtccag tacgaagatc
    15541 cagaagatgt aaaggacaca gtataaagcc cagaaataca ggtgctgata acgatggagg
    15601 cgatatcttt tctgatgctc gctcatccgc atccagggct ggatgtcgat atcgtcatcg
    15661 atgccctcca cattcgtata ggcatgatgg acgacattgt gtttcatatt ccacatgaag
    15721 ctgttcccgc cgaggatatt cagcgaaaac gccgccacat gattgaccca tttgtatttg
    15781 gaaaaactgc catgcgcccc gtcgtgcatg acattgaagc cgatggcggc cacgacgccc
    15841 cccagtaaaa cactttccac gatggcccag aaagcgttcg gcgtaaagaa caccaggtgg
    15901 atatagataa aaatgaaagc ggccatcagg ataacagcct tcagaaagag gtcataatta
    15961 cccgtctggg ccttacccac ttcctcgaaa taactgctga tcctccgttt taactcaccg
    16021 tggaaagatt gagttgccgt cgaaaatttg ggcaatgaca tgacgatacg attgaagctt
    16081 taataattct gcaaaattag acttttaaca gggtatttcg tgttaagacc tgcacaatat
    16141 tcccacacct gcacattcaa cggtaaatct gcgcttttgt tgctggaaat catggccatt
    16201 aggtatctat ctgtaccaat gcgccaaaat cccttatacc gatcattttt tgcgtcgtaa
    16261 acccctcggc ataggccact ccgatcattt tcccgaactg ctggtgacgc ccgatcagcg
    16321 attgcaggaa ttccggactt gagatatagg tttggggctc gttttcacca taggtagtgg
    16381 aaaagaattg cgagctataa gcctcgatgg attgcacttt cgtgtcgaac accggagtaa
    16441 tgtcgaacac aaaatccgga tccctatacc ggtcctggat ataatggaac acatacttgg
    16501 ggcgccagtg ctcctgcggc cgccctgcct caccggtggt ctccactttc cggagaccgc
    16561 tgtaaaaaca cgattcgacg atcagatgac ccgcccgccc atgatcggga tgcctgtcct
    16621 cgagcgcatt ggcaagtacg atctcgggcc ggtatttacg tatcgcgcgg atgagcagcc
    16681 gttgatgctc ttcgtcattg cgaaaaaacc catccgccat gcccaggttc tcccgaacat
    16741 ccagctgaag gattgcagcc gaacgggccg cctcttgtcc acgcagctcc gccgtgcccc
    16801 tggtacccag ctctcctcgt gtaaggtcga tgacgccggt cttcttgcca ttgcgttttt
    16861 ccatgaggag cgtgccggca catcccagct ctacgtcatc cgggtgaacg gcgatagcca
    16921 gtatatctaa cttcgttgat ttcatgcgcg tgaagatagc tcattttgtc atttccctat
    16981 tgtagagtac cttcgcggat caaaatcaag gaatccatat gccttacgat gtagtcgtcc
    17041 tcggcagcgg taccggcggt tacccggccg cgatccgcgc ctcacagctt ggctttaagg
    17101 tagcaattat tgaaaaagaa aatctcggcg gcgtttgcct caactggggt tgtatcccca
    17161 ctaaggccct tctgaaaagc gcgcaggtct atcaggacat cctccatgca aaggaattcg
    17221 gtatcgatgc caccggcacc gccgattttc cggccgtcat caaacgcagc cgcggcgtag
    17281 cggataaaat gagcaagggc gtccaatttc tcatgcgtaa gaataagatc gacgtcgtca
    17341 ccggttatgg taagctgaaa gccaaaggac agatagaagt caccggcgcc gacggcaaga
    17401 cccagatcgt cgaaggcaaa catatcatcg tcgccaccgg cgcgcgaagc cgcgagctgc
    17461 ccaatctcaa acaggatggc aaaaaggtga tcggctaccg cgaggccatg accctgcccg
    17521 cacagcccaa aagcatcatc gtcgtaggca gcggcgccat cggcgttgaa ttcgcctatt
    17581 tctattacag catgggcaca aaggtcacca tcgtcgagtt cctcccccgc atcgtaccgg
    17641 tcgaagacga agagatttcc aaagagctcg aaaaaacata caagaaatat ggcatcgaag
    17701 tcctgaccag cagcgaggtc accagcctcg acaccggtgg caacggtgtc aaagcaaagg
    17761 tcaaaacccc caccggcgag gtcaccctcg aagccgatat cgtcctcagt gccgtcggca
    17821 tcgccgccaa tatcgagaac atcggtctgg aaacgatggg catcaagaca gaaaaaggca
    17881 aaatagtcgt cgataaatac tatcagacca gcgtaccggg catctactcc gtcggcgact
    17941 gcgtccccgg tcaggccctc gcacacgtct cttccaaaga agggatcatc tgtgtggaaa
    18001 acattgcctg gaaggagaag aaatatgccc atcagccgga aacgctggac tataacaata
    18061 tccccggctg cacatattgc acaccggaga tcgcctccgt aggcctcacc gaaaagcagg
    18121 cccgcgacgc cggctatgag gtcaaagtcg gcaaattccc gcttaccgcg tccggcaaag
    18181 ccacaggtgt cggccataat gaaggcttcg tgaaagtcgt cttcgacgct aaatacggcg
    18241 aatggcttgg cactcatgcc atcggctata atgtaacgga gatcatcacc gaaacagtgc
    18301 tcggccgcaa actcgaaacg acctaccacg aggtcctcga cgccgtccac ccgcatccta
    18361 ccatcagcga ggcagtcaaa gacgccgtcg aggcggccta cggggaagcc atacacttat
    18421 aacataacct tcatacctaa aaaagaggcc tctggcctct tttttgttta tattagctcc
    18481 atggacagcc gaaattccag catcatctat ttcatcctgg gtctctacac cctgatgatc
    18541 agttggcact acaaccacaa ctggatcctc ctgctcatcc actggatcat ctggcccgtc
    18601 tacctcgtct acgaacttct catcggccat ctttcccacg atctctggcg caccatccca
    18661 ctgggctatt tcaaataacc ccttcctgta acggccacga ccaaccgcca tcggccgccc
    18721 tcccggcaaa tacaccgctc cccgcttata cttcgatggt ctcgcccgat gcgggcagca
    18781 gcagcttctt ccccgccgcc gcaaagtctg ctttggcctt ctccgcatcg atagcaatga
    18841 tcggccatgt attataatgt acccccacga cggtagcaca cttgacgaaa tccgccgcat
    18901 gaatggcgtc ggctacatcc atcgtatagt tgcccccgat gggcataacc gcgaattgca
    18961 gtttcgccca caacgggatc aattgcatat ccatcgtcag cgccgtatca ccggcgatat
    19021 aaaaatcacc ctctgtcgta ttgaaaacaa atcccaatgg atttccggca tagctgccgt
    19081 cactgaagga agaagaatgg atggcattga ccgcgcgtac ctttccgaat tcgaatggga
    19141 ccggtccgcc atggtttatc gggtgcacct tttccaaacc ctgcttgccc agccaccccg
    19201 caatctcggc cgcggccaca caagtcgcac ccgtcctttt cgccacactc acagcatcgg
    19261 cggtgtgatc cccatgccca tgcgtcagga ataggtaatc agctttaact ttatcaatat
    19321 caatgttttt gagagcagga ttgccgttga agaaggggtc gaataacagg gtcttcccct
    19381 tgaccgtaac agaaaaggtg gcgtgtccgt aaaatgtaag tttcatcctg gtaatttaaa
    19441 catctcataa aacaaaacac agttataaaa gttcgcaacc gctatcttta tccactaaaa
    19501 ccaaactttc atgcagcaca acctttccaa aaaatccatc ttggtggcca tcaccctcct
    19561 ggtgaccggc ctcagtttcc gggccatcgc ccagcccatc acgggcgatg agatcaaaac
    19621 ccaattcgta aaagattggg agcgggcaaa atcctacacc ctggattacc tcaacacgat
    19681 gcctcccgac aaatattcct ttaaggcggt agacagcatt cgcagctttg cgcagcaaat
    19741 gttgcacctg gccagcggca atgtgggcct tatctcacaa gccaccggcg aaaagacgga
    19801 tttcccccgt aacctcgaaa aaagccctac ggcccagaac aaagattccg tggtctggta
    19861 cgtcacccgc agctatgatt tcgcgatcga aagcattcag aaactcgacc cgaacaaata
    19921 tggcgaaatg cttggacagg atccccgaaa ggtctcccgc ttcgcctggc tcctgaaagc
    19981 cttcgaacac cagacccatc atcgcggcca aacgaccatc tatatccgtc tgcagggcat
    20041 ccggccaccc aacgaaagat tattttaaac gcgcgcagtg tcaccggccc aactcagcga
    20101 tcagctggaa tcagcatcct ccggcggtca tccgggcatc ctccggcgtt catcagggca
    20161 tccggccgga tcatttcggc cgggcccgct catactgcaa tggccaatgg atatccgcat
    20221 atcctaactc attagccgca tgcagcggga aataaggatc acgaaggatc tgccgcgcca
    20281 tcaccacaag atcggcctga cccgtcacca gcacggtctc tgcctgcatc gtacccgtga
    20341 tcattccgac ggctgcggta agaatacccg tagcggcacg gatctgccgg gagaattcaa
    20401 cctggtaacc gggccctgcg ggtatttgct gatacggcac cagcccgccg gatgaacagt
    20461 cgatgacatc taccccccgg tcttttaaca acgccgccaa ccggaccgca tcctccggcg
    20521 accatccacc gtccgcccaa tccgtggcgg agatccttac gaacaaggga taaaccaccg
    20581 gccacacccc ccgcaccgca tctaccacct ccatgaccag ccgaaccctg ttcccgaaca
    20641 cacccccata ttcatccgaa cgccgattgc tcagcggaga caggaattga tgcaacaggt
    20701 aaccgtgcgc cgcatgcagc tcgatcacct tgaaacccgc cagcatggcc cggcgcgccg
    20761 ccgccccgaa ttcatccact accctgcgta tatccgcaac agtcatctcg accggcaatc
    20821 cataacgctc actgtacgcc accgcactcg gccccagcac acgccagccc ccttcacttt
    20881 cctttaccaa cccgccgcca ttccagggct cctttgtgct ggccttcctg cccgcatgcg
    20941 ccagctgtat accgatctca ctcccttgtg accggacgaa cttgacgatc cggcgtaacc
    21001 cttcgatatg ctcgtctttc cagatgccga gatcgaaggg agatatcctc ccttccgccg
    21061 agacggccgt cgcttccgta aataccagcc ccgcaccacc caccgcacga ctacccagat
    21121 ggacaagatg ccagtcttcg gccttgccgt catgcgacga atattgacac atagggctga
    21181 cgacaattcg atttccgaga ataatgtccc ggatctgtaa gggggtaaac agtttgctca
    21241 tattagaagc gaaggtccta aatttaggta taattttcac catctatgta ccatgtccgc
    21301 tgggttcttc cggcaatatg cgccgctact acctttgtga cggcctacaa accgctcccg
    21361 gcatccgcgc cggcggaaac ctgccaatct tccaccgagg tcctcgttgc cgcagccgcc
    21421 gatctgcgat ttgcgatgga ttcgctggtg gccgccttct ccctcgaaaa ccccgacatc
    21481 acggtcaagc ctacctatgg ctcctcaggt aatttcttcc aacagataca gaacgacgcc
    21541 cccttcgatc tcttcttttc cgccgatgtc gattaccccc gccagctgaa agaaaaaaac
    21601 ctcaccctct ccgatatcta tatttatggg accggccaac tcgtcctctg gagcaaaacc
    21661 ctcgatccca acaaaaaaca aatgaatacc ctgctcgaca aatccgtcac caaaatagcc
    21721 attgccaacc ccgcccatgc tccctatggc aaacgggcgg aagaaagcct tcatcactat
    21781 aacctctatg acaaagtgaa agacaagctc atctttggtg agaatatcgc ccagacagcc
    21841 caatatgcac aaagcggcgc ggcggaggtc ggaatcatcg cgctctcact ggcggtatcc
    21901 ccgtcaatgc gccagacagg cggcaaattc tggctcatcc ccgcaaaagc ccatcaaccc
    21961 ctgctgcagg gattcgtcct gctcccacat tcgaaaggca acgccggcgc cgaaaaattc
    22021 gcccgcttct gcaattccca tacagccaaa gacgtcttgc acaattttgg gttcacccaa
    22081 ccttcctcat aaataggcaa attcaatgat cgactggtta cccatctggc tcagcctccg
    22141 gctcgccctc gttacgaccg ccatcctctt cgtactgggc atcccgctca gctaccggat
    22201 tgcgtatagc cggcgccgct ggtccatttt cgctgaggcg tgcatcagcc tgccaatggt
    22261 attgccgcct accgttctcg gcttttacct gctgctggcc ttcagccccg ccggctcatt
    22321 cggccacttc ctggaccact atctcgatct ccgtctcgtc tttaccttcc gcggactcgt
    22381 tgttgcctcg ctcatctata gccttccctt catggtcaat cccctgctcg ccggattcaa
    22441 aaatcttccg ccgcacctcc gcgaggcttc ccgaaccctc ggcaaatccg actggaccac
    22501 tctccggcgg atactcctcc ctaatatgcg tccttcactg gtcaccggcg ccatcctctc
    22561 tttcgcccat accatcggcg agttcggcgt ggtcatgatg atcggcggcg ccatccccgg
    22621 tcgcaccagg gtcgcttcag tagccatcta cgacgaggta caatcgctga attacccgct
    22681 ggccaatcac tatgcgatcg tattgctgct cttctccttc agtatcctcc tcgtggtgta
    22741 cgccatcaac caccgtcaat ccaaagccgt cctatgagct cgcctcccgc caaccttatt
    22801 ttccgggcga caaaaatgct ccataccgca cagggtcccc aactgttcga tgtcagcttc
    22861 gatctgccac aaggccgcct gttggccgtt tacggatcct ccggctccgg taagaccacc
    22921 cttctccgca tcctcgcagg cctgacagag acggcttccg gtcatatcga attggatgga
    22981 gagatctggc tcaaccatcc caacaatccc aacgttccca gcaacctcaa cgttcccagc
    23041 aacctcaacc atcccaacag ccccaaccat cccaacaacc ccaacgatcc ccacggctcc
    23101 aatcgtccac ccggccccgg tcgctacaca agccttccca cccgccaccg ctccatcggc
    23161 ttcgtattcc aggacttcgc tctctttccc catctcacag tccgcggtca gctggaattc
    23221 gcccttccca aacacgccga ccgatccatc atcgatgagc tcctcacgct catggagctg
    23281 gaagaactgc aacaccgccg ccccgccctt ctctccggag gacaacaaca acgcatcgcc
    23341 atcgcccgcg cgatcgcacg acgcccgaga ctcctgcttc tcgacgagcc gctgtcggca
    23401 ctcgacgacg agatgcgctc acgactccag aactatatcg gaatagccca ccgccattac
    23461 aaactcacta ccatcctcgt cagtcactac cttcccgaga tcatcagcct ttccgatgaa
    23521 gccatcatcc tcgaaggagg caggatcctc gaccgcggca tccccgaaac cctcttcccg
    23581 aaagatagga tcatcggcga tatccggctc aacggagaga tcattgacat cacaaacgcc
    23641 ggcgacacct ccatcatcca cgttctttcc gccggctcca cgatcaaaat cgtcactcca
    23701 tccggcgcca atatgcacat cggacaacac gtcaccatcg cctaccacca tttcaacccc
    23761 gtcatccttc ccgcgacctg ataaattttt cttattttag gtgccaatcc ttatttatgc
    23821 cgtcgctatc cgtctgggaa aaagaaagtt ttttcgcccc cgccgatgtc atcatcgccg
    23881 gcagcggcct ggttggcctg tggagcgcct actacctgaa aaaacgcgcc ccttccctca
    23941 ccatcaccat cctcgaacgc ggcattatcc ccacgggcgc cagtacccga aacgccggct
    24001 tcgcctgctt cggcagtctt acggagttaa taaaagacct ccgatcctca ggcgaggacc
    24061 gaatgctcca actggtggaa atgcgttata aaggcctcaa aaagatcaaa aagactttag
    24121 gcggaaaagc catcggatgg gaaaagtacg gcggctacga gctcattgca gaaggcgatg
    24181 cagacgagat ccgctcgcag atcgatctcc tcaaccacct cctgaaaaag gtcaccggac
    24241 aacagaagac cttccggttg cagaacgacc tgatctcccg tttcggcttc tcgggaacgc
    24301 agctgctcat cggcaatcct tccgaagccc aattgcactc cggacgacta tgccaggcct
    24361 tgctctgcct ggtccaatcc atgggcgtca cagtactcaa caacatcgaa attacaggat
    24421 tcgaaaaaac gggaggccag ctactcttac ataccagcct tcccttcccg t
//
LOCUS       DASYSV010000508         6122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2537588, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000508 DASYSV010000000
VERSION     DASYSV010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537588"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="VGQ51_15800"
     CDS             complement(<1..1818)
                     /locus_tag="VGQ51_15800"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9038099.1"
                     /translation="MRYVQYPLLLVLQFSAYTSFSQPRNLKFAHIGTEAGLSANNVTS
                     ILRDSQGFMWFGTRDGLNRYDGYDFDIYRNTPEDPQSLSSNFVTSVIQDRNGDLWVGT
                     WGGGLNRFDRAKRRFVRYAGIIHAEFINSLMEDSEGRIWIGSSDHGAWLLDPRTGKVL
                     LFTSVAGNPASLSDNDVLTIAEDHAHQIWLGTSRGGLDRYDPGSRSFIRYTHRPGDEN
                     SLASNTVERILEDGRHRLWIGMRDGGLDLLDPLHGTFRHFRNDRGRASSLVSNVVLAL
                     EDDDQGNLWIGTENGGISILNKDMDRFITCAHDDVDNTSLSNNSIDALYRDPQGNLWV
                     GTADGGIDLYNKSVNSIDHYKHTASPESLANNNVLDILEDGDHLWVGTDGGGLDEMDL
                     TTGKFRHFVHGAGSGKSISGNYVLSLCKDAKGNLWTGTWGNGITVIGKDGKVIHQYKH
                     DDADSNSLGGDNVYAITDDGEGTVWVATYGDGLNQFNTKTGGFHHYKHVASDPNSPGS
                     DRLNTLLSDSNGILWIGTYDGGLDRLDEYTGIFRHFLHETGKNSLSNNTVNFIYADSR
                     HNFWVCTADGLNLMDRKTGQFKVYGIKDGLPSSVIFGILE"
     gene            complement(1913..3133)
                     /locus_tag="VGQ51_15805"
     CDS             complement(1913..3133)
                     /locus_tag="VGQ51_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEV9038100.1"
                     /translation="MKRIPSIDVARGLVMVIMALDHSRDLLHINAAQSPTNLATTTTI
                     LFLTRWITHLCAPAFVFLSGTSAFIYLFRASSNTSAPNRASATTSAPNRTSDDLRSRR
                     RFLLTRGLVLILMEFTIVNFGMFFDVRFRLFMFEVIATIGAGMFLLSFLSRLPLKVIM
                     ALSALLIFGHDLFSPGMLPPTGPINFIGSLLLSLNAYTTGADRVFVIAYPVLPWLGIM
                     LAGFAAGRLFIMPVEKRKSIFRKLGFSLLTLFILLRLIDRYGDPIHWATQKNAVFTFL
                     SFINVNKYPPSLLFTLVTLGILLLVLSAAEGRDNRTTRFLLIYGQVPMFYFIIHFYIL
                     HTILIITVLLQGYHWADLPFGGGQFGRPPGAGLPLAAVYAIWIAVVITLYPLCRRYGQ
                     YKQSHREKKWLRYI"
     gene            3189..4421
                     /locus_tag="VGQ51_15810"
     CDS             3189..4421
                     /locus_tag="VGQ51_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEV9038101.1"
                     /translation="MKSMSGIIAVILLLGMMAEGRAQGFLKTQGRLIVNENGEKVILR
                     GMGLGGWMLQESYMLKLGGVGPQYLMRRKISDLIGPEKTAAFYEAWLADQTRRIDIDS
                     MAAWGFNSVRLPMHYHLFTLSVEEEPVAGKNTWLQKGFALTDSLLGWCKANHLYLILD
                     MHAAPGGQGNDFNISDRDSTKPSLWESAANRQKLIALWRRLAERYANEPWIGGYDIIN
                     EPNWGFEDPSDKHGLKETKNAPLRQLMMDITAAIRSVDRRHMIIIEGNGWGNNYRGVL
                     PQWDDNMVLSFHKYWNPNTEEAIHHYLGLREQYNIPLWLGESGENNNTWYRECVRLVE
                     SHDIGWSWWPLKKIGANNPLEVPMTPEWRRVVDYLTGKGPRPTESEAEAGLHSLLHSV
                     RAENNIYHKEVTDALMQR"
     gene            complement(5000..5167)
                     /locus_tag="VGQ51_15815"
     CDS             complement(5000..5167)
                     /locus_tag="VGQ51_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038102.1"
                     /translation="MSTTRKKNIRIRRKKPSLDASNVVVVTHTTEKDTLVPEKLKKAN
                     EMLSKSKMMNS"
     gene            5442..>6122
                     /locus_tag="VGQ51_15820"
     CDS             5442..>6122
                     /locus_tag="VGQ51_15820"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV9038103.1"
                     /translation="MKPFHSYRNPAVAIDLVVFGYADGELSVLLLNRKEAPFEGGWTL
                     PGAFLQMEERFSDTCSRILQTKLGMDRLYLEQLYSFDEPERDPRGRVISVAYYALVNP
                     SKLRIAAGRMANDVQWMNVGKMPALGFDHRNIFKTAMQRLRSKILYYPVGFELLDEQF
                     TMTELHELYECILGISMDRRNFRRMILAADYILPTGAKREGLQNRHPELYVFNKKLKP
                     NNFHLNIPL"
BASE COUNT         1492 a         1436 c         1750 g         1444 t
ORIGIN      
        1 ttccaggatg ccgaagatca ccgagctcgg aagcccgtct ttaatgccgt atactttaaa
       61 ctggccggtc ttccggtcca ttagattcaa accatccgct gtacagaccc agaaattatg
      121 ccggctgtcg gcataaatga aattgacggt gttgttgctc aggctatttt ttcctgtctc
      181 atgcaagaaa tgtctgaata tgccggtgta ttcatcgagc ctgtccagcc cgccgtcata
      241 agtgccgatc caaaggatac cgttgctgtc ggagagcagg gtattgagac gatcggagcc
      301 gggactgttg ggatcggacg caacgtgttt ataatggtga aagccgccgg tctttgtatt
      361 gaattggttc agtccgtctc catatgtcgc aacccatacc gttccctcac cgtcatcggt
      421 gatcgcgtat acattgtccc cgccgaggct attgctgtcg gcatcgtcat gtttgtattg
      481 atgaattacc tttccgtcct ttccgatcac cgttataccg ttaccccagg tgcctgtcca
      541 cagattgcct ttggcatcct tgcacaggct gaggacataa ttgccgctga tgcttttacc
      601 ggatccggcg ccatgtacaa aatgcctgaa tttccccgtc gtcaggtcca tttcatcgag
      661 cccgcctcca tccgtaccca cccacaaatg atcgccgtcc tcgaggatat cgagcacatt
      721 gttgttggca agactttccg gcgaagcggt gtgtttgtaa tgatcgatac tgttgacgct
      781 cttattgtac aggtcgatgc cgccgtctgc cgtgccgacc cacaaattgc cctgggggtc
      841 gcggtacagc gcatcgatag agttattact gagactggta ttgtcgacgt cgtcatgtgc
      901 gcaggtgatg aaacgatcca tatccttatt caggatactg atgccgccat tttccgttcc
      961 gatccaaaga tttccctgat cgtcgtcttc cagcgcaagg accacattcg ataccaggct
     1021 gcttgcgcgg ccgcggtcat tccggaaatg ccggaaagtt ccatgcagcg gatccagcag
     1081 atccagcccg ccgtccctca ttccgatcca caaccggtga cggccgtctt ccaggatcct
     1141 ttcaaccgta ttcgacgcca gggagttctc atcacccggg cggtgtgtat agcggataaa
     1201 ggaccgcgac cccggatcat accggtccag tcctccccgt gacgttccga gccagatctg
     1261 atgagcgtgg tcttccgcga tggtcaggac gtcgttgtcg ctgagactgg cgggattgcc
     1321 cgcgaccgag gtaaacagca gcaccttgcc tgtcctgggg tccaatagcc aggcaccatg
     1381 atcgctcgaa ccgatccaga tacgaccttc ggagtcctcc atcagcgaat tgataaattc
     1441 ggcatgaatg atccccgcat atcgtacgaa acgccgcttt gccctgtcaa aacgattcaa
     1501 tccgccgccc caggtaccga cccagaggtc gccgttacgg tcctgtatca ccgatgtcac
     1561 gaaattgctg ctgagacttt gcggatcctc gggggtattc ctgtatatat cgaaatcata
     1621 cccgtcatac cggttgagac catcccgggt accaaaccac atgaaaccct ggctgtcccg
     1681 caggatggag gtcacgttat tcgctgaaag gcctgcttcg gtaccgatat gggcgaattt
     1741 gagattccgg ggttgagaaa aagaggtata tgctgaaaat tgcagcacca gaaggagtgg
     1801 atattggaca tacctcatac ggaatgattc ggagttactt tttaaaacgc agattggacc
     1861 ccggcagatg tgccagatag atattttttt gttatatgat aagtgtaaac acttatatat
     1921 atcgtagcca tttcttttcc cgatgcgatt gtttgtattg tccataacgt cggcagaggg
     1981 gataaagtgt gatgacgacg gcgatccaga tggcataaac ggcggcgagc gggaggcctg
     2041 ctccgggggg acggccgaat tgtccaccgc cgaagggaag gtcggcccaa tgatagccct
     2101 gcagcagaac ggtgatgatc aggatagtgt ggagaatata gaagtgaata atgaaataga
     2161 acatcggtac ctgaccatat atcagcagga aacgggtggt ccggttgtca cggccttcgg
     2221 cggccgagag caccagcagg aggatgccga gcgtcacgag ggtgaagagc agtgaaggcg
     2281 gatatttgtt gacgtttatg aaggaaagga acgtgaacac ggcatttttt tgtgtcgccc
     2341 aatggatcgg atcgccataa cgatcgatga gacgcaacag gatgaaaagg gtcagtaggg
     2401 agaaaccgag ttttcggaag atgctctttc ttttttccac gggcatgatg aagaggcggc
     2461 ctgccgcgaa gcctgccagc atgatcccca accagggcag aacgggatag gcgataacga
     2521 acaccctgtc ggcgcctgta gtgtaggcgt tcaggctgag cagcagtgat ccgataaagt
     2581 tgatcgggcc ggtgggcggc agcatacccg gtgagaacag gtcatggccg aaaatgagga
     2641 gcgctgaaag cgccatgatg acctttagag gaaggcggga taaaaaggat agcaggaaca
     2701 tgcctgctcc gatggtcgcg atgacctcga acatgaagag acggaaacga acgtcgaaga
     2761 acatgccgaa gttgacgatc gtaaattcca tcagaatgag cacgagaccc cgtgtcagca
     2821 ggaaacggcg gcgggagcgg agatcgtcgg atgtgcggtt gggggcggat gtggtcgccg
     2881 atgcgcggtt gggggcggat gtgttcgacg atgcgcggaa caggtatata aacgccgatg
     2941 tccccgataa aaagacgaaa gccggagcgc acagatgcgt gatccagcgg gtaagaaaaa
     3001 gtatcgtcgt agtcgtggcc agattggtag ggctttgggc tgcatttata tgcagcagat
     3061 cgcgcgaatg atcgagcgcc atgatgacca tgaccagacc gcgggcgaca tcgatggaag
     3121 gaatacgttt catgagcgtt agccgttggg gtgagactaa agttaaaaaa aaggattact
     3181 tttcggttat gaaaagtatg agcggtatca ttgcggtcat tctgttgttg ggcatgatgg
     3241 ctgaaggccg ggcgcaagga ttcctgaaga cgcagggtcg gctaatcgtc aatgagaatg
     3301 gagagaaggt gatcctccgg ggtatgggac tcggcggctg gatgttgcag gaaagctata
     3361 tgcttaaatt gggtggcgta gggccccagt acctgatgcg cagaaagatc agcgatctga
     3421 taggaccgga gaagacggct gctttttatg aggcctggct tgcggatcaa acgcgccgga
     3481 tcgatattga ctcgatggca gcgtggggtt tcaattccgt ccgtctgcct atgcattatc
     3541 atttgttcac cttgtcggtg gaggaggagc cggtcgcggg aaaaaacacc tggctgcaaa
     3601 aaggttttgc cctcaccgat agcctgctcg gctggtgtaa ggccaatcat ctttatctca
     3661 tcctcgatat gcacgccgcg ccaggcggcc agggcaacga tttcaatata tcggatcgcg
     3721 attccaccaa gccgtcgctt tgggaaagcg cggccaaccg gcagaagctg atcgcgctgt
     3781 ggcgccggct ggctgaaagg tatgccaatg aaccctggat cggcggatac gatatcatca
     3841 acgaacccaa ctggggtttt gaggacccct ctgataaaca tggactaaaa gaaacgaaga
     3901 atgcaccgct gaggcaatta atgatggata tcacggccgc catccggtcg gtcgaccgcc
     3961 gccatatgat catcatcgag ggaaatggct ggggcaacaa ctatcgcggt gtcctgccac
     4021 agtgggatga taatatggtt ctgagctttc ataaatactg gaacccgaac acggaggagg
     4081 cgatacatca ttacctcggg ctgcgcgagc aatacaatat tccgctgtgg cttggtgagt
     4141 cgggtgagaa caataataca tggtaccggg aatgtgtccg gctggtagaa agtcatgaca
     4201 tcggctggtc ctggtggcct ttgaaaaaga tcggggcgaa caatccgctg gaagtaccga
     4261 tgacgccgga atggcgccgt gtcgtggatt atctgacggg taagggcccg cgcccgacgg
     4321 aatcggaggc ggaagccggg ttacacagtc tgcttcacag cgtgcgggcg gagaacaata
     4381 tttatcataa ggaagtgacg gatgcgttga tgcagcgatg aaagggatgc catttattgc
     4441 tggcgaagga attgcccggg tcagggccga aggaattgtt ccagctgttg aaaggctccg
     4501 atgaacattt tgtacttgct gtccgagcag tcgttcgcgt atctccgctt cgctttcctt
     4561 gtgagtggcg gggatacgcc atttgttatt ttcgggagag aaggggtctt ctcccggacg
     4621 ggatgacgga agggggaggc cttcgaaata tccgatgcgg cccctggcga caacgagctg
     4681 catttcttcc ggtgtgattt tcctgatgga atattgataa gtcttgtgtt gctgattggt
     4741 aagaaagagg ttttgatcct ttcgagccca ggtgccatat tcatatcggc cgaaatcagc
     4801 ggtatatgag ccgtcggagc gtaattcaag aaaattggcg ggggtgaggc ctccgtcgtc
     4861 tcccgagccg gagccatagg tgtagaacca gaggtgtggg agggggttag aaatcatcga
     4921 gcatccgccg aaggcggtca gcagggaaag aaagaggaga tagaagaggg atttcataag
     4981 cagcgaatgc catcgagaat caactattca tcattttgga tttgctcaac atctcgttag
     5041 cctttttgag tttttcagga actaaagtat ccttctcagt ggtgtgagtg acaacgacta
     5101 cattggaggc gtccagggac ggctttttcc ttcttatcct gatattcttt tttctggttg
     5161 tcgacatata acaaatttaa agaattcttt ttataaaaaa ggcacaataa atattgggag
     5221 agttaggagc gaagaatatc tcctgaaagc gtggcccggt ctttatgaga gccgtgatat
     5281 gcacgtgtca tgatgctcac atttattggt tttcggcagg ggtaaattta ctaactaatt
     5341 tagtattgcc aggagaaata tgattttttg aaattttggg tcccccttct tgcaaggatc
     5401 aatagttttg ttttactttg cgtcagatta acgcaaacaa catgaaaccc tttcactctt
     5461 acaggaaccc tgcggtggcg atcgacctgg tggtcttcgg gtatgccgac ggcgaattgt
     5521 cggtgctgtt gctcaacagg aaggaagctc cttttgaggg cggatggaca ttgccgggag
     5581 cttttttgca gatggaggag cggttttcgg atacctgttc ccgcatcttg cagaccaagc
     5641 tgggaatgga ccggctgtac ctggaacagc tatacagctt tgacgaaccg gaacgcgatc
     5701 cgcggggcag ggtcatatcg gtggcctatt atgcattggt caatccatcc aaattgagga
     5761 tcgcagcagg aagaatggcc aatgacgtcc aatggatgaa cgtcggaaag atgccggcac
     5821 tgggattcga tcaccgcaat atcttcaaaa cggccatgca aaggctgcga tcgaagatcc
     5881 tttactaccc ggtgggtttc gagctgctgg atgaacagtt cacgatgacc gaattgcacg
     5941 agctgtatga atgcatcctg ggtatatcga tggacaggcg caatttccgg cgtatgatac
     6001 ttgccgccga ttatattttg ccgaccggag ctaaacggga agggctgcag aacaggcacc
     6061 cggaattgta tgttttcaat aaaaagctaa agccgaataa tttccacctt aatatccctt
     6121 ta
//
LOCUS       DASYSV010000509         5440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_229824, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000509 DASYSV010000000
VERSION     DASYSV010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229824"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VGQ51_15825"
     CDS             <1..803
                     /locus_tag="VGQ51_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV9038104.1"
                     /translation="ELVARVLQPHRKKIFIATKFGFRADASGRLNSFDGSPAYLKQAV
                     EGSLRRLRTDVIDLYYAHRIDPNVPVEDMVGAMADLVREGKVRFLGLSEASPASIRRA
                     HAVHPISALQSEYSILTRDVENEILPLCEELHISFIPFSPLSRGLMTNTVDPSTLGEG
                     DFRRNLPRYQGEHADNNVRLAAGFADIAAAKGCTPAQMALAWVLARGSHIIPIPGTKR
                     RKYLVDNAGAMDVTMTAADFTQIDQLLKRFPNIGDRYNESNRRFVDKN"
     gene            1112..3430
                     /locus_tag="VGQ51_15830"
     CDS             1112..3430
                     /locus_tag="VGQ51_15830"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEV9038105.1"
                     /translation="MVFFLFCPAFPGQVIAQPILSAGAPAELSIREAGTHSLRITLKP
                     LTDTVDLPFTPALADRHWPAPVIDLKEVSSPVRKQVGDLQVEVTASPLTVTVSTKDGA
                     PVQRLVFDDSTLSFALDEAPVLGLGEGGHRPVRGGNWRKASIEFDRRGRLQDMQPRWQ
                     GDAYGSRNPVPLLIGTKGWALFVANPWGEIDLRNPDHGIYLPWKPTAADTVQQNERNQ
                     SLALAKGLPPASSVIPGLYDIFVFDAHHPADLMKDISDLTGPAVIPPKWALGYMQSHR
                     TIKDDRYILGIVDSFRSKKIPVDAVIYLGTGFTPQGWNTRQPSFEFNPAVFHRDPAGV
                     LADLHARHVKVVLHMVPYDRDKLPTLHGTIPASPGETTDAGHIQTYWQKHIPLMMAGA
                     DAFWPDEGDWFNLYERIERHKLYYQGPLSTFPDTRPWSLHRNGYLGVARWGGWVWSGD
                     TESSWKTLEGQIAVGINHSLSLSPYWGSDIGGFYPNSEKTGELYARWFQFGAFCPSFR
                     SHGRTTMTILPWGWGQGDRGDLETSTRPGPDSATRNVLLSSMNDPRIEPVVRKYDELR
                     YQLLPYTYTLAWEARVTGMPLMRALWLHYPDDTIARALGSEYLWGRDLLIAPVFEKGA
                     TSRAVYLPAGTWYDWWSGDTLSGGRTVNRDVDLSIMPIYVRAGAIIPSDTIRQYTSQP
                     VTAPTTLRIYPGADGDFTLYDDDGISQQYLRGRASYIRITWDDHNRTLTLRPASPAGL
                     PVRRHFIVQDMSRPASAAHIIFYKGRSMTIHM"
     gene            complement(3421..4263)
                     /locus_tag="VGQ51_15835"
     CDS             complement(3421..4263)
                     /locus_tag="VGQ51_15835"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEV9038106.1"
                     /translation="MMISFDLIYPDQSAIPYRSMTFPDGQPHIKMDVGVAERIDRKEP
                     LRIFTRLNNANDLLLALLVKNSLDYLGFEHIELHVSYLLAGRMDRVMLDGEPFSLKVV
                     ASMINQGQFKKVLLFDPHSEVSTALIDRSYAVANHAFVRDALKDYASDHSGEALCLVS
                     PDAGALKKIHKLAQALGIGNVVECMKERDVRTGALTKFNAMTDGLHGQTCFIVDDICD
                     GGGTFAGTANVLKEKGAGRVNLIVSHGIFSKGTVIEDIDEVYTTDSYRKVEGVNCFSI
                     GRYI"
     gene            complement(4272..5000)
                     /locus_tag="VGQ51_15840"
     CDS             complement(4272..5000)
                     /locus_tag="VGQ51_15840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV9038107.1"
                     /translation="MARTYVIGDIHGALRALQQVMERVAPAREDRLIFLGDYVDGWSQ
                     SRQVIDQLIELESRYECIFIRGNHDAWCVEWLEGWGASPDWLFHGGAATMKSYAGITT
                     EERGRQLEFFNRMRSYFEEDGRLFVHAGFSSVHGPSAEHYESNFFWDRTLWETALAVD
                     DRIPRDSPFYPQRLRLYREIYIGHTPTTNYDFDVPMQRSNVWNVDTGAAFTGRLTMMD
                     IDTKKYIQSDIVQQLYPGETGRNK"
     gene            complement(5013..>5440)
                     /locus_tag="VGQ51_15845"
     CDS             complement(5013..>5440)
                     /locus_tag="VGQ51_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HEV9038108.1"
                     /translation="SKSAMNNVCFSKEDLEEVVVTNDKQRFSFSDGGTRIRANQGHSI
                     AVELQLKPQKPPQVLFHGTVGKFLNSIKEKGLQKMDRHHVHLSADRSTAERVGNRRGE
                     AVILTVKSYEMGWDGFEFFLSENGVWLTDHVPVRYIEFF"
BASE COUNT         1127 a         1762 c         1332 g         1219 t
ORIGIN      
        1 aggagctggt ggcccgcgtg ctccagccac accgcaaaaa gatcttcatt gcaaccaaat
       61 tcggattccg ggcagacgcc tccggacgtc tcaatagttt cgatggctca cccgcctatc
      121 tcaaacaggc cgtggaagga agcctccgtc gtctccgtac cgacgtcatc gatctgtatt
      181 atgcccatcg catcgatccg aatgttccgg tcgaagatat ggtaggcgcc atggccgatc
      241 ttgtacggga aggaaaagtg cgcttcctgg ggctttccga ggcctctccg gccagtattc
      301 gccgcgccca cgccgtacat cccatcagcg cgctgcagag cgaatattcc attcttaccc
      361 gcgatgtcga aaacgagata ctgccgctat gcgaagagtt gcacatttct tttattcctt
      421 ttagtccgct ctcccgcgga ctcatgacca atacggtcga tccgtcgacg cttggagagg
      481 gcgatttccg gcgcaatctg ccccgctatc agggagagca cgcggacaat aacgtccgtc
      541 tggccgccgg ctttgccgat atcgcagccg ccaaaggatg cacgcccgca cagatggccc
      601 tcgcctgggt tttggcccgc ggctcgcata tcattccgat ccccggcacc aaacgccgga
      661 aatacctggt ggacaatgcc ggcgctatgg acgtcacgat gactgctgcc gatttcacac
      721 agatcgacca gctgctgaaa cgttttccca atatcggtga tcggtacaac gagtccaacc
      781 ggcggttcgt cgataagaat tgaagacctg ctgcttccgg ccgctcccga ctgctcccgg
      841 cctgcttcag gccgctcctc gcggcgccgg ccgctccctg ggctccggca ggacaaaggg
      901 caataccact taagtaccgg tattttgtcg ttttctattg gagagtgggg aggtatgggt
      961 gttagaacct accggttcgt ggtatccggc gttgggtccg ggttaatttc tgtttgaagt
     1021 tagctaaaat gtttttgccc gttttacttt tattccggga tttttgttta ctcatttaaa
     1081 aagcttgccg atgccggtcc acccccgcat aatggtattt tttctttttt gccccgcctt
     1141 tccgggacag gtgattgcac agccgatcct gtcggcaggc gctccagccg aattgtccat
     1201 ccgggaagcg ggcacacaca gcctccggat tacattgaag ccgctgaccg acacggtcga
     1261 tctgcccttt accccggctc ttgccgaccg ccattggccc gctcccgtta tcgatctgaa
     1321 agaagtttcg tcgcccgtcc gcaaacaggt gggcgatctg caggtggaag tcaccgcctc
     1381 tccactgacc gttaccgtta gcacgaagga cggcgcgccc gtccaacggc tcgtattcga
     1441 tgactccacc ctctccttcg cgctggatga agcgcccgtg ctgggtctcg gcgaaggcgg
     1501 gcaccggccg gtgcgcggcg ggaattggcg taaggcctcc attgaattcg atcgccgggg
     1561 aagactgcag gacatgcagc cgcgctggca aggcgatgcc tacggctcca ggaaccccgt
     1621 tccgttgctc atcggcacaa aaggctgggc attgttcgtg gcaaatccct ggggtgagat
     1681 cgatctgcgc aatcccgacc atggcatata cctgccctgg aaaccgaccg ccgccgatac
     1741 cgtccagcag aacgaaagaa accagagtct cgcactcgca aaaggtctcc ctccggcctc
     1801 ttcggtgatc cccggtttgt atgacatatt cgtcttcgac gcgcatcacc cggccgatct
     1861 catgaaggac atctccgatc tgacgggtcc cgccgtgatt ccgccgaaat gggcgctcgg
     1921 ctatatgcaa tcgcatcgga cgataaagga cgatcgatat atccttggca tcgtggacag
     1981 cttccgttcg aaaaaaatac cggtcgacgc ggtcatctat ctcggaacag gctttacgcc
     2041 gcagggttgg aataccaggc aaccttcctt cgaattcaat ccggccgttt ttcaccgcga
     2101 ccccgccggg gtgctcgccg atctccatgc ccggcatgta aaggtggtac tgcacatggt
     2161 cccctatgat cgcgataagc tgccgaccct ccacggcacc attcccgcct caccgggaga
     2221 aaccacggat gcggggcata tccaaaccta ctggcaaaaa catattccgc tgatgatggc
     2281 cggcgcggat gccttctggc ccgacgaagg cgactggttc aatctatacg aacggatcga
     2341 gcgccacaag ttgtattatc agggtcctct ctccactttc cccgacaccc gcccctggag
     2401 cctccatcgc aatggctatc tgggtgtcgc ccgatggggc ggctgggtct ggtcgggaga
     2461 caccgagtcc tcctggaaga cgctggaggg acagatcgcc gtcggcatca accattccct
     2521 cagcctgagc ccctactggg gctccgacat cggcggcttc tatcctaata gcgagaagac
     2581 gggtgaactc tacgcacgct ggttccagtt cggagccttc tgcccttcct tccgcagcca
     2641 tggccggacg accatgacca tcctgccctg gggctggggt cagggggacc gcggagacct
     2701 cgagacctcc acacggcccg gccccgacag cgcgactcgc aacgtcctcc tctcttcgat
     2761 gaacgacccc cgcatcgaac ccgtcgtacg gaaatatgac gagctgcgct accagctcct
     2821 accctacaca tatacgttag catgggaggc ccgcgttacg ggcatgcccc tgatgcgcgc
     2881 gctctggctt cattatcccg acgataccat cgcccgcgct ctgggcagcg aatacctctg
     2941 gggtcgcgat ctcctgatcg ctcccgtctt cgaaaaaggc gccaccagcc gcgccgtata
     3001 tctgcctgca ggcacatggt acgattggtg gtccggcgat accctctccg gcggcaggac
     3061 cgtcaaccgc gatgtcgatc tttccatcat gcccatctat gttcgtgccg gagccatcat
     3121 tccttccgat accatccgcc aatacacctc ccaacccgtc accgccccca ccaccctccg
     3181 catctatccc ggcgccgacg gcgacttcac cctctatgac gacgacggca tcagccaaca
     3241 atatcttcgc ggccgggcct cctacatccg catcacctgg gacgaccaca accgcaccct
     3301 caccctccgt cccgcctccc ccgccggtct ccccgttcgc cgccacttca tagtgcagga
     3361 tatgagccgg ccggcttcgg cagcccacat catcttttac aagggcagat caatgactat
     3421 tcatatgtag cggccgatcg aaaaacaatt caccccttcc accttcctgt atgaatccgt
     3481 ggtatacacc tcatcgatgt cttctatcac cgtccctttg ctgaatattc catgactaac
     3541 gatcaggttc actctacccg cgcccttttc tttcaggaca ttcgccgtac ctgcgaaagt
     3601 tccgcctccg tcgcagatgt cgtccacgat gaaacaggtt tgcccatgaa gcccgtccgt
     3661 catcgcgttg aatttggtca gggccccagt ccggacgtcc cgctctttca tacattccac
     3721 gacatttccg atgcccaacg cttgcgccag tttgtgtatc ttcttcaagg ctcccgcatc
     3781 cggcgacacg aggcaaagcg cctcccccga atgatcgctg gcataatcct tcagcgcatc
     3841 ccggacgaag gcatggttcg ccaccgcgta tgaacggtcg atcagcgccg tcgatacctc
     3901 cgagtggggg tcgaacagga gcaccttttt gaattgcccc tggttgatca tcgacgccac
     3961 cactttcaga gaaaatggtt cgccgtcgag catgacccgg tccatccgcc cggcaagcag
     4021 ataagagaca tgcagctcga tatgttcgaa tcccaggtaa tccaggctat tcttcaccag
     4081 caacgccagc aacaggtcat tggcattgtt cagacgggtg aagatccgca gcggctcctt
     4141 tctgtcgatc ctttcggcca ccccaacgtc cattttaata tgcggttgcc cgtccggaaa
     4201 ggtcatcgac cggtagggta tggccgattg gtcggggtag atcagatcaa agctaatcat
     4261 cattcttggg tttatttgtt tcgtcccgtt tcgccgggat acagttgttg cacgatatcg
     4321 ctttgaatat attttttcgt gtcgatgtcc atcatcgtta gccttcccgt aaacgcagcg
     4381 ccggtatcta cattccacac attgctacgt tgcatgggga catcgaagtc gtaattggtc
     4441 gtcggcgtgt gaccgatata gatctcccga taaagccgga gacgttgtgg atagaacgga
     4501 ctatcgcgcg ggatacggtc atccactgcc agcgctgttt cccagagcgt cctatcccag
     4561 aaaaagttgc tttcgtaatg ttcagcggac ggcccatgca cagaggaaaa acctgcatgc
     4621 acgaacaacc gcccgtcctc ttcgaaatac gaccgcatcc gattgaaaaa ctccagctga
     4681 cgtccacgtt cctctgtcgt gatacccgca tagctcttca tcgtcgccgc tccgccatga
     4741 aacagccagt ccggggaggc accccatcct tccagccatt ccacgcacca tgcatcatgg
     4801 tttccccgga taaagatgca ttcatacctg ctctccagtt caatcagttg gtcgatcacc
     4861 tgccgcgact gcgaccaccc gtcgacatag tcccccagga agatcaaccg atcctcccgc
     4921 gccggagcca ccctttccat cacctgctgc aacgcccgca gcgccccgtg aatatcgccg
     4981 attacatagg tccttgccat ttcatacgtg ttttagaaaa actcaatata cctaaccggc
     5041 acatggtccg tcagccaaac cccgttctcc gaaagaaaaa attcaaaccc atcccatccc
     5101 atttcatagc tcttaacggt caggatcaca gcctctcccc tgcgattacc caccctttcc
     5161 gccgtcgacc tgtcagcgct caaatgcacg tgatgcctgt ccattttttg cagccccttt
     5221 tctttgatgc tgtttaaaaa tttgcccacc gtgccatgaa aaagtacctg aggcggtttt
     5281 tgcggcttca attgcaattc tacggcaata gaatgcccct ggttggccct gatcctcgtg
     5341 ccaccgtcac taaatgaaaa ccgctgttta tcgttagtca caaccacctc ttccagatct
     5401 tctttcgaaa aacaaacatt attcatcgca gatttagaga
//
LOCUS       DASYSV010000510         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1258102, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000510 DASYSV010000000
VERSION     DASYSV010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258102"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VGQ51_15850"
     CDS             complement(<1..524)
                     /locus_tag="VGQ51_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038109.1"
                     /translation="MRLPYRFLFVLLATTHIHTSCAAQTGDDTLTAGVSHSPKDGIVL
                     HGATRDLSNLDISTRTLAAGRSFTLPPGNADYLLIVREGNIEIAHKNLGPGGIALFPA
                     GDRPDVRQSGASAAVFYLFSFRSRSPAQPGRAQKAGPPVILDWSQLPMKKTGKGESRA
                     IFSRPVAWLANINM"
     gene            complement(527..1921)
                     /locus_tag="VGQ51_15855"
     CDS             complement(527..1921)
                     /locus_tag="VGQ51_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9038110.1"
                     /translation="MKKNTINRRKFLGSSLKAGVAATILPTAVFPSIVPSTVFGKYAP
                     SNRINVGAIGTGRISRVHDLPGIWQFDTARIMAVCDLDSNRVTAAKDLVNDYYSKKTG
                     KAYDGVATYHDHRELLLNKDIDAVVVSTPDHWHALLAIHAVEAGKDVYLQKPASLTIS
                     EGRIMADTIKRSGRIFQIGSQQRSSPQFRYAAELVRNGRIGELKTVYVGLPGDPSGNP
                     EAEMPVPPNLNYDAWLGSTPMVYYTEKRVHPQVGFDRPGWLRCEQFGAGMITGWGAHH
                     IDSAHWGMDTEYTGPVEVWGHAEFPTSGLWDVHGIFRTEALYANGVHMIVSNELPNGI
                     KFEGTKGWIFVTRGDYRATASDPVPDANGVKPLEASDPTIIRSVIGPNETHLYVSNDH
                     HGNWLECIKTRKAPITNAEIAHRACSACLIHHIVMKLKRKVYWDPVAERFKNDDEANA
                     RLSRPQRPPYIVKA"
     gene            complement(1925..>4484)
                     /locus_tag="VGQ51_15860"
     CDS             complement(1925..>4484)
                     /locus_tag="VGQ51_15860"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEV9038111.1"
                     /translation="HLCAQFPFQNPDLPTGQRVDDLVGRMTLEEKIGQMMNAAPAIPR
                     LGIPEYNWWNECLHGVARAGLATVFPQAIGLAAAWDENMQFRVATAISDEARAKYQDF
                     IRHDKRLIYEGLTFWSPNVNIFRDPRWGRGQETYGEDPFLTGRLAVQFVKGLQGDDPH
                     YFKTIATLKHFAVHSGPEPERHFFDAQADERDFRETYLPQFKMGIEEGKAYSVMCAYN
                     RFRGEPCCSSNELLTTILRKEWGFQGYVVSDCGAIDDIYLHHKVVATPEAAGALAVKA
                     GCDLECATTFKHLNEAVEKGLVSEKEIDVAVKRLFTARFKLGMFDPPDRVKYASIPYS
                     VVDSKQNRDLALDAARRSIVLLKNENHTLPLKKDIRTLAVIGPNADQWQMLLGNYNGL
                     PSRAYTPLEGIRSRLGDAHVLYAQGCELAEGLPTFVKVPVAVLSGLHADYYNHHDFSG
                     GILFSGKDAQLDANWGEGAPRKDMNGDDFGVKWSGEITPDASGSYQIGIITTCKVNLY
                     LDDSLVANTSYHFRDEFNDPRLRNSKSIHLEAGKHYKLRVEAGETYGDARVELVWSKQ
                     GADRNEELMQEALTTAKKADAVVLCMGLSSRLEGEEMDVNIEGFKGGDRTSLDLPKVQ
                     ERLIQAVTGLGKPVILVLLNGSAVSVNWEDQHIPAILEAWYPGEEAGPAIADVLFGDY
                     NPAGRLPVTFYHGLKDLPDFGDYAMAGRTYRYFKGDVLYPFGYGLSYTTFHYDGLNVQ
                     QPSNPGDSVHVSVNVKNTGALEGDEVAELYVGAIGASVPVPIRSLWGFRRIHLAPGET
                     RTLHFAVSPDAFTVIDDKMQRVHLTGNYDISVGGGQPGSPRPATSNTLTTSLHF"
BASE COUNT          910 a         1134 c         1419 g         1021 t
ORIGIN      
        1 tgcatattga tattcgccag ccatgcgaca gggcggctga agatggccct gctttcaccc
       61 ttgccggttt ttttcatcgg cagttgcgac caatcgagaa taaccggcgg tccggctttt
      121 tgagcccggc cgggttgcgc cggtgatcgg gagcgaaagg agaacaggta aaaaacggca
      181 gcggaagcgc cggattgacg aacgtcgggt ctgtcgccag cggggaaaag ggctatgccg
      241 cccggaccta aatttttgtg agcaatttcg atattgcctt cacgcacgat aagcagatag
      301 tcggcgttgc cgggaggtaa ggtaaaagat cggccggcgg caagggtccg ggtgcttata
      361 tcgagattgg agagatcgcg cgtagcgccg tggagaacga tgccgtcctt cggcgaatga
      421 gagacgcctg cggtcagggt atcgtcgcct gtttgggccg cgcaggaagt atggatatgt
      481 gtcgtggcga gcaggacgaa gaggaaccga tatggtaaac gcataatcaa gctttgacga
      541 tatagggcgg tcgttgggga cgggaaagtc ttgcattggc ctcatcatcg ttcttgaagc
      601 gttcggcgac cggatcccaa tatacctttc ttttcagctt cattacgatg tgatgtataa
      661 gacaggcgga gcaggcgcga tgggcgatct ccgcattggt gatgggggct ttccgggttt
      721 tgatacattc cagccagttg ccgtgatggt cgttgctgac atagaggtgg gtctcgttgg
      781 ggccgatgac acttcggatg atcgtcgggt cgctggcctc gaggggtttg acgccgttgg
      841 catccggaac gggatcgctg gcggttgcgc ggtaatcgcc gcgggtgacg aagatccagc
      901 ctttggtccc ctcgaacttg atgccattgg gcaactcatt gctgacgatc atatggacgc
      961 cattggcata gagcgcttcc gtccggaaga tgccgtgtac gtcccagaga ccgctggtag
     1021 gaaactcggc atggccccag acctcgacgg gtccggtata ttcggtatcc atgccccagt
     1081 gggcggaatc gatgtgatgg gctccccagc cggtgatcat tcctgcgccg aactgttcgc
     1141 agcgcagcca gcccggccgg tcgaaaccta cctgcggatg gactcttttt tccgtgtaat
     1201 agaccattgg cgtggagccg agccatgcgt catagttgag attgggcggg acgggcattt
     1261 cggcttccgg attcccggac ggatcgccgg ggagacctac ataaactgtt ttcaattcac
     1321 cgatccgacc gttgcgaacc agttcggcgg catagcgaaa ttggggagaa gaacgttgct
     1381 ggcttccgat ctggaagatc cgacccgacc gcttgatcgt atcggccatg atccttcctt
     1441 cggagatagt gagcgaagcg ggtttttgca gatagacgtc cttccccgcc tcgacggcgt
     1501 gtatggcgag gagggcatgc cagtgatcgg gggtgctgac gacgacggca tcgatatctt
     1561 tattgagcag cagctcgcgg tgatcgtggt aggtggcgac cccgtcgtag gctttccctg
     1621 tctttttcga atagtaatcg ttgacgagat cttttgcggc cgtgacgcgg ttggaatcca
     1681 gatcgcagac ggccatgatc ctcgccgtat cgaattgcca gatgccgggt agatcatgaa
     1741 cgcgcgagat gcggcccgta ccgatggccc cgacattgat ccggttgctc ggtgcatatt
     1801 tgccgaagac ggtggatgga acgatagaag gaaagacggc ggtgggaagg atggtcgccg
     1861 cgacgccggc tttgagcgac gaaccgagga atttccttct gttgatggta ttttttttca
     1921 tatatcagaa gtgaagtgat gttgtgagtg tattggacgt ggcgggacgc gggctacccg
     1981 gctggcctcc gccgactgaa atatcgtagt ttccggtgag atgtacgcgc tgcattttgt
     2041 cgtcgatgac ggtgaacgcg tcggggctga ctgcaaaatg gagtgtgcgt gtttcgcccg
     2101 gcgcaagatg tatacgccgg aaaccccata gcgatcggat cgggacgggg acggaggcac
     2161 ctattgcacc gacatagagc tcggcaacct catcgccttc gagggcaccg gtatttttta
     2221 cattgacgga gacatggacg gaatcgccgg ggttcgaggg ctgctgtacg ttgaggccat
     2281 cgtaatggaa agtcgtatag ctgagcccgt agccgaaagg atagagcaca tcacctttga
     2341 aatagcggta ggtccgtcct gccatcgcat agtcgccgaa atcggggagg tcttttagcc
     2401 cgtgatagaa agtgacgggc aggcgtcccg cgggattgta gtcgccgaag agtacatcgg
     2461 caatggccgg acctgcctct tccccgggat accaggcttc gaggatggcg gggatgtgct
     2521 ggtcttccca gttgacggaa accgcgcttc cgttgagcag cacgaggata acgggtttgc
     2581 cgagaccggt aacggcctgg atcaggcgtt cctgcacttt tggcagatcg agcgaggtcc
     2641 ggtcgccacc tttgaagcct tcgatattga cgtccatctc ttccccttcg agccgggacg
     2701 aaagacccat gcacagcacg actgcgtcgg cttttttcgc cgtggtcaat gcctcctgca
     2761 ttaattcctc attgcgatcg gcgccctgtt tggaccatac caactccacc cgggcatcac
     2821 cataggtctc gccggcttcg acgcggagct tgtaatgttt cccggcttcg agatggatgc
     2881 ttttggagtt tctcagccgc ggatcgttga actcgtcgcg gaaatgatag ctggtattgg
     2941 ccacgagtga atcgtcgaga taaaggttca ccttacaggt ggtgatgata ccgatctggt
     3001 agctgccgga tgcgtcgggg gtgatctcac cggaccactt gaccccgaag tcatcgccgt
     3061 tcatatcctt gcggggagcg ccttcgcccc agttggcgtc aagctgcgcg tctttgcctg
     3121 aaaagaggat gccgccgctg aaatcatggt ggttgtaata gtcggcgtga agacctgaaa
     3181 gcaccgcgac gggaaccttg acaaaagtgg gcaggccttc tgccaactca cacccctgcg
     3241 catagaggac gtgagcatcg ccgagacggc tgcggatgcc ttccagcggt gtataggccc
     3301 tggagggaag gccgttatag ttgccgagca gcatctgcca ctggtcggca tttgggccga
     3361 tgaccgccag cgtgcggatg tcttttttca acggcagggt atggttctca tttttgagca
     3421 gtacgatgga gcggcgggcg gcgtcgagcg ccagatcgcg attctgtttg ctgtctacca
     3481 ccgaataggg aatagaagcg tattttaccc ggtcgggcgg atcgaacatg cccaatttga
     3541 aacgtgcggt aaataatctt ttaacggcga cgtctatctc tttttcggag acgagccctt
     3601 tttcaacggc ctcgttgaga tgtttgaacg tcgtggcgca ttcgagatcg catcccgcct
     3661 ttacggcgag ggcaccggca gcttcgggcg tggcgactac cttatgatgc agatagatat
     3721 cgtcaatagc gccacagtct gaaacgacat agccctggaa cccccattcc ttacgaagga
     3781 tggtcgtgag caactcgttg ctgctgcagc agggctcgcc gcgaaaacgg ttataggcgc
     3841 acatgacgga atatgctttg ccttcctcga tgcccatttt gaattgcggg agataggtct
     3901 cgcgaaaatc acgttcatcg gcctgcgcgt caaagaaatg gcgttcgggt tcgggaccgc
     3961 tgtggacagc aaaatgtttg agtgtcgcga tggttttgaa ataatgcggg tcgtcgcctt
     4021 gcaggccttt gacgaattgt acggcgagac gtcccgtgag gaagggatct tctccgtatg
     4081 tttcctggcc gcggccccag cgcggatcgc gaaagatatt gacgttgggc gaccagaagg
     4141 taagcccttc atagatcagg cgtttgtcat ggcggatgaa atcctggtat ttggcgcggg
     4201 cttcatcgga gatagcagtg gctacgcgga actgcatatt ttcatcccag gcggcggcca
     4261 ggccgatagc ctgtgggaaa acggtggcaa gacctgcacg tgcgacgcca tggagacatt
     4321 cgttccacca gttgtattcc ggtatgccga gacgggggat ggcgggggca gcattcatca
     4381 tctggccgat cttttcttcg agggtcatgc ggccgacgag gtcgtcgacg cgctggcctg
     4441 tgggcaggtc ggggttttga aaaggaaatt gcgcgcagag atgg
//
LOCUS       DASYSV010000511         4745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_616117, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000511 DASYSV010000000
VERSION     DASYSV010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616117"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VGQ51_15865"
     CDS             <1..625
                     /locus_tag="VGQ51_15865"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEV9038112.1"
                     /translation="VVGVMAQVAVAAGKQPSDITAYNGLSSGLAISDIYNKKDSASAL
                     NVARYSTATGEPMIIVGYPELCFNIAEAINRGWISGDANAYYQNGIQASMKFYQQTGG
                     QITDGQISAYLAQSQVQYNGDLNNILIQKYLSFFNNSGWESFYNILRTGVPVLAVGPG
                     NGNNNKIPDRWMYPQTEYTYNAQNVKAAIQTQFGGADDRNGVLWIYQ"
     gene            678..3494
                     /locus_tag="VGQ51_15870"
     CDS             678..3494
                     /locus_tag="VGQ51_15870"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEV9038113.1"
                     /translation="MYFSRRLFLFLLLLFPVRLLRAQTSQEKPWVWWFWLGSAVTKES
                     IDFQLQSFHRSGFGGVVIISTYGVKGYEDRDISFRSPRWFDMLHYTINEAAALHMGVD
                     MGLSSAWPFGGPSVTRAQGAKMLKSCTSFHSAGGALRRQLLPEGSQDFVLAVLAEGPG
                     GRSVDLTPGVVSGGRLEGVLPSGDWMIYVLTGANTDQQVKRSGPGGEGLVLDQFSGPA
                     LADYLHGYDSVPGELSGVRSVMNDSYEVYRADATDSILKEFKARRGYSLVPYFPLLFD
                     SSGSGRGKEGLPGSEEASRVLCDYRETISDLLLDGFMHTWSKWSNGHGMLSAEQAHGA
                     PANILDLYGAADIPETESFGPAHFPIPNVRVDKDIRRDRYKWPDILMFKFASSAANVN
                     GRRLCSAETATWLTNHFRMALSQLKPQLDQLFVAGINHTMLISATNVPLDVPFPGWRF
                     YPAPDFGHYAAFYDYLPDLSGYITRCQHLLQQSRPDNDALVYFPIYDYWSEAPKDLGV
                     LATFDAIPGKWGDRFAFSATARKLKNGGVSFDYISDQQLQMLRFDGRSLITTGGNKYK
                     VIVIPECRRMPVKTLRRLADLAAQGAPVLFENGMPKDVPGWKDWRRRLGELKGLQAEM
                     LHTPRVRISHDLAGDCVRAGCMDEHFAGLEFIRKRTAGGKLYFVANLDSVFSCDWITP
                     GVGATELERYDPMTGARGLLIRKKGPEGRYSFRLELLPGQSCFLVPAAGEKAEKTATY
                     FSGSSSVVLDGAWKIRFEKGAPFLPGPIETRVLKSWTEMGDSAARAFSGTACYELHFD
                     LPAGLDRCRKIRLNFTDLRDMADVELNGHRVGRVWSVPYQLDIDRREFKEKGNDLRIQ
                     VTNLATNRIIWMDRQKIPWKTFYIADPVKGTFDASGWDLQPSGIIGPVSLTGGTMEPA
                     VGKPAVGKPAVGKPALGKPALGKG"
     gene            3523..>4745
                     /locus_tag="VGQ51_15875"
     CDS             3523..>4745
                     /locus_tag="VGQ51_15875"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEV9038114.1"
                     /translation="MDSNMIKSKTIVCLLCGLLSVRISMAQRSNPPSEERPWLWWYWL
                     GSEVTPEGIQQQLNAFHQAGFGGVSVSATYEVDGHKAQSIPFMSKRWTDMLEYTASTG
                     RKLGMGTDVALASAWPFGGPNVTPAMAAQRMAGGKLLNAAGGDVIDLPVYKVGSKTLL
                     ALVAIADSGRHLDLTKDVDADGVLHFTFPAGQWEVYSLFSEPTGQMVKRSGVGGAGLV
                     MDHFDANAVEKYLTRFDSLFLEPSHLRATFNDSYEVYGADCTPRILQEFKKRRGYDFS
                     RYLADWYSPGMTDTRQRLLCDYRETIGDLLLEDFIRPWNRWAHAHGVKTVEQAHGSPA
                     NWLDLYAACDIPQTESFGASTFSIPGVTQDKDYPVESFGRPDKLLMKFASSAGNFTGK
                     KLVSSETATWLGNHF"
BASE COUNT         1022 a         1073 c         1529 g         1121 t
ORIGIN      
        1 ggtcgtgggt gtgatggcgc aggtggccgt cgccgccggg aaacaaccgt ccgatattac
       61 ggcgtataat gggctcagca gcgggctcgc catatccgat atttataata agaaggatag
      121 cgcctcggcg ctgaatgtgg cgcgttattc tacggccacc ggagagccga tgatcatcgt
      181 cggttatccc gagctgtgct tcaatattgc ggaagcgatc aacaggggat ggatctccgg
      241 ggatgcgaat gcgtattatc agaatggtat ccaggcctcg atgaagttct atcagcagac
      301 gggggggcag atcacggatg ggcagataag cgcctatctc gcacaatcgc aggtacagta
      361 caatggcgat ctgaataata tccttattca gaaatatctg agctttttta ataattcggg
      421 ttgggagagt ttttataata tcctgcgcac cggggtgccg gtgctggccg tagggcctgg
      481 caatggcaac aataacaaga tccccgatag atggatgtat cctcagacgg agtatactta
      541 caatgcccag aatgtgaagg ctgcgattca aacgcagttt ggtggggcgg atgacaggaa
      601 cggggtgttg tggatctatc agtagtggaa aaccgggggc cgcgccggag gcgcggccct
      661 tataaatttt ttttgcgatg tatttttccc gccggttatt tcttttcttg ttgttgctgt
      721 tccccgtcag gcttttgcgt gcccagactt cacaggaaaa gccatgggta tggtggttct
      781 ggctgggaag cgcagtgacc aaagaaagca tagattttca gcttcaatct tttcaccgga
      841 gcggattcgg cggtgttgtc attatttcga cttatggcgt gaagggttac gaagacagag
      901 atatatcatt tcgaagtccc aggtggttcg acatgctgca ttatacgatc aatgaagctg
      961 ccgcgcttca tatgggggtg gatatggggt tgtcttcggc gtggcctttc ggggggccat
     1021 cggtgacgag ggcgcaggga gctaagatgc tcaagagttg tacttctttt cattcggcag
     1081 gaggcgcgtt gcgccggcaa ttgttgccgg agggatcgca ggattttgtg cttgccgtgc
     1141 ttgcggaggg gccggggggg cggtccgtcg atcttactcc gggggtggtt tcgggggggc
     1201 ggctcgaggg ggtgcttcct tcgggcgatt ggatgatcta tgtacttacg ggtgcgaata
     1261 cggatcaaca ggtgaagcgg tccgggccgg gaggtgaagg gctcgtgttg gatcaattca
     1321 gcgggccggc attggcagat tatttacacg gctatgatag tgtgccggga gaattgagcg
     1381 gggttcggtc ggtgatgaat gattcgtatg aagtgtatag ggccgatgcg acagatagta
     1441 tattaaaaga atttaaggca aggagggggt actcgctggt gccgtatttt ccgctgttgt
     1501 tcgacagcag cgggtcaggt cgggggaagg aagggttgcc agggtctgaa gaggcttcca
     1561 gggtgttgtg cgattatcgc gagaccattt ccgatctgtt gctggacggg tttatgcata
     1621 cctggtcgaa atggagcaat gggcacggca tgctgtccgc cgagcaggcg catggtgcgc
     1681 ccgccaatat cctcgatctc tatggcgcgg ccgacatacc ggagacggaa tccttcggtc
     1741 ccgctcattt cccgatcccg aatgtccgcg tcgacaagga tatccggagg gaccggtata
     1801 aatggccgga tatattgatg ttcaaatttg cgtcttccgc cgccaatgtc aatggccgcc
     1861 gtctgtgctc ggccgaaacg gcgacatggc tgacaaatca tttcagaatg gcgctgtcgc
     1921 aactgaaacc acaactggat caattattcg tcgccggcat caatcacacc atgctaatca
     1981 gcgcaacgaa cgtgcctctc gacgtgcctt tcccggggtg gagattttac cccgccccgg
     2041 acttcgggca ttatgccgcc ttttatgatt atctgcccga tctcagcggg tatattacgc
     2101 ggtgtcagca tctgttgcaa caatccaggc cggataatga cgcgctggtg tactttccca
     2161 tttatgatta ctggtccgag gcgccgaagg atctgggggt cctggcgaca ttcgatgcga
     2221 tacccggaaa atggggagac cggtttgctt tttcggccac ggcgagaaaa ttgaagaacg
     2281 gtggtgtatc cttcgattat atctccgatc aacagttgca gatgctgcgc tttgacggca
     2341 ggtcgctgat aactaccggc ggcaacaagt ataaagtgat cgtcattccg gaatgccggc
     2401 ggatgccggt gaagaccttg cggcgtctgg ccgatctggc ggcgcaggga gcgccggtgc
     2461 tgttcgagaa cggtatgccg aaggatgttc ccggatggaa ggattggcgc cggcggttgg
     2521 gtgaattgaa aggattacag gcggagatgc tacatacgcc gagggtgcga atttcgcatg
     2581 atctggccgg tgattgtgtc cgggccggat gtatggacga gcatttcgcc ggacttgaat
     2641 ttatccggaa aaggaccgcc ggcggtaagt tatattttgt ggcgaatctc gatagtgtgt
     2701 tctcgtgtga ctggatcaca cccggagtcg gagctacgga actggagcgt tatgatccga
     2761 tgacaggggc acggggtctg ctgataagga agaaagggcc ggaaggcaga tacagtttcc
     2821 gtctggaatt gctgcccggt caatcgtgtt tcttggttcc tgctgccgga gaaaaggcgg
     2881 agaagacggc gacatatttt agcgggagtt cgtctgtcgt tctggatggg gcctggaaga
     2941 tccgttttga aaaaggcgcg ccctttttgc cggggccgat cgaaaccagg gttttgaaga
     3001 gctggacgga gatgggggac agtgctgccc gggcattcag cggaactgcc tgttatgagc
     3061 ttcatttcga tctgcccgcg ggattggacc ggtgccggaa gatacgtttg aactttactg
     3121 atctgagaga tatggcggac gtggaattga acgggcatcg cgtcgggcgg gtatggagcg
     3181 tgccatacca gctggatatc gaccgccggg aattcaaaga gaagggaaac gatctgcgga
     3241 tccaagttac taacctggcg accaaccgga tcatctggat ggaccggcag aagataccct
     3301 ggaagacctt ttatatcgcc gatccggtta agggaacatt cgatgcttcc ggctgggatc
     3361 tgcagccgtc gggaatcatc gggccggtga gtctcaccgg tgggaccatg gagcccgcgg
     3421 tggggaaacc cgcggtgggg aaacccgcgg tggggaagcc tgcgttgggg aagcctgcgt
     3481 tggggaaggg gtaagggatg cggggaaata aaaattagaa ttatggatag caatatgata
     3541 aagagtaaga cgatcgtctg tttgctgtgt ggacttctca gtgtgagaat ctcgatggct
     3601 cagcgttcga atccgcccag cgaggaaaga ccctggcttt ggtggtattg gttaggcagc
     3661 gaagtgacac cggaagggat ccagcaacag ctgaatgctt ttcaccaggc agggtttggc
     3721 ggggtaagcg tgagcgcaac gtatgaagtc gatgggcaca aggcacagtc tatcccgttt
     3781 atgagcaaac gctggacgga catgctggaa tatacggcgt cgaccggcag aaaactgggt
     3841 atggggactg atgtcgcgct tgcatcggcg tggccgtttg gcggaccgaa tgtgacgcct
     3901 gctatggctg cgcagagaat ggcgggtggt aagctattga atgccgcggg cggcgatgtg
     3961 atcgatctgc cggtctataa ggtgggatcg aagacccttt tggcgctggt agcgattgcg
     4021 gattcggggc ggcatcttga tctgacaaaa gatgtcgatg cggacggggt gctccatttt
     4081 acctttccgg cgggccagtg ggaagtctat agtttgttct cggaaccgac gggacagatg
     4141 gttaaacgtt caggggtcgg cggtgcgggc ctggtgatgg accatttcga tgcaaatgcc
     4201 gtcgaaaaat atctgacgcg atttgactca ctgttcctgg aaccctccca tttgcgggcg
     4261 acgttcaatg attcgtatga ggtatatgga gcggattgca cgccgcgcat cctgcaagag
     4321 ttcaaaaagc ggcgcggata tgattttagt cgttatctcg ccgattggta tagtcccggt
     4381 atgacggata ccaggcaacg tttgctctgc gattaccgcg agacgatagg ggatcttcta
     4441 ttggaagatt ttatccgtcc ctggaaccgc tgggcgcacg cgcatggtgt caagaccgtc
     4501 gagcaggcgc acggttcacc cgccaattgg ctggacctct atgcagcctg tgatattccg
     4561 cagaccgaat cgttcggggc cagtaccttc agtatccccg gcgtaaccca ggacaaggat
     4621 tatccggtcg aatctttcgg acgtccggat aaattgctca tgaaatttgc ctcttccgcg
     4681 gggaatttta ccgggaagaa gctggtctcc tccgagacgg ccacctggtt ggggaatcat
     4741 ttcaa
//
LOCUS       DASYSV010000512         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1767543, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000512 DASYSV010000000
VERSION     DASYSV010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767543"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..515)
                     /locus_tag="VGQ51_15880"
     CDS             complement(15..515)
                     /locus_tag="VGQ51_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV9038115.1"
                     /translation="MATVEFNQLLVNNAEFLKPFAITLTRDSEAAKDLFQETLYRALA
                     NKDKYNVGTNIKAWLYTIMRNIFINNYRRKAKQNTIFDSTPNEFLLNSAQMAVPVSVE
                     SNLRVKDIQSAIHKLPEIFRNPFLLYFDGFKYHEIAEMLQEPLGTIKSRIHFARKLLK
                     TQIQRF"
     gene            complement(807..1550)
                     /locus_tag="VGQ51_15885"
     CDS             complement(807..1550)
                     /locus_tag="VGQ51_15885"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEV9038116.1"
                     /translation="MFSLFKKRPPDPSGALAGLGCDIHSHLVPGIDDGSPDMETSLRL
                     IRGLVDLGYKKIITTPHVIGDIFPNTPDIIRAGRDSVAAALRENGIDVEFHAAAEYLM
                     DDRFARALDAGGPFLTLKDNLILVELSFAVPAINLKELLFQLQVKGYQPILAHPERYL
                     YFGANKGWYDQICDAGCLFQLNLLSLIGYYGKDARQLAEYLLKKGYFDFLGTDLHHTR
                     HLHALGSSRHLFQTVDRLLQTGKIQNPRL"
     gene            complement(1534..2469)
                     /locus_tag="VGQ51_15890"
     CDS             complement(1534..2469)
                     /locus_tag="VGQ51_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEV9038117.1"
                     /translation="MNPKEKIYIAGHRGMVGSAISRRLQKEGFTNIVTRTSAELDLRD
                     QAAVREFFAEETPDHVFLAAAKVGGIMANNTYRAEFLYDNLLIESNIIDAAYRNGVRK
                     LLFLGSSCIYPKMAPQPLKEEYLLTGTLEDTNEPYAIAKIAGIKLCDAYRSQYGCNFI
                     SVMPTNLYGPNDNYDLTTSHVLPALVRKFHEAKLNSAPFVTIWGSGKPRREFLHADDL
                     ADACYFLMQGYDEPGLVNIGTGEDLEIGELAALIGRIVGYTGEIRHDTSKPDGTPRKL
                     MDVSKLNGLGWKASISLEEGLRRVYEEYQSHVLAV"
     gene            complement(2474..3589)
                     /gene="gmd"
                     /locus_tag="VGQ51_15895"
     CDS             complement(2474..3589)
                     /gene="gmd"
                     /locus_tag="VGQ51_15895"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEV9038118.1"
                     /translation="MSKVALITGVTGQDGAYLSELLLKKGYIVHGIKRRSSLFNTDRI
                     DHLYQDPHESHVNFFLHYGDLTDSTNLIRIIQETQPDEIYNLAAMSHVHVSFETPEYT
                     ANADGIGTLRILEAVRLLGLVKKTKVYQASTSELYGLVQAVPQSENTPFYPRSPYAVA
                     KLYAYWITVNYREAYGMYATNGILFNHESPLRGETFVTRKITRGVAKIGLGLQDKIYL
                     GNLDARRDWGHARDYVEAMWRILQQPEPEDYVIATGITTPVRDFVRMAFAEIGVELEF
                     KGSEEKEIGIVASSSNPDFIVPVGKEVVAVDARYYRPTEVDLLIGDASKANTKLGWTP
                     VYTLPMLVKEMVDADVQLFRKERVLKESGFHIKNQYE"
     gene            complement(3600..4493)
                     /locus_tag="VGQ51_15900"
     CDS             complement(3600..4493)
                     /locus_tag="VGQ51_15900"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEV9038119.1"
                     /translation="MKQFFKMVRFIWNHPLASKNRPLAFRQLLSWQFGQRMRPGPQQM
                     SLVEDSILIVEKGMEGATGNIYTGLLEFEDMAFLLHILRREDVFADVGANVGVYTILA
                     AKNAGADVISIEPIPSTFKRLQRNVAANKVAEQVVLKAFGASASAGTLLFTASEDSVN
                     HVVKNTEDSNGRPVIEVPVRTLDEMLEGRTPVLLKIDVEGFEWPAFLGASRLLADPGL
                     KALIVELNGSGSRYGFQEEQLHRLLLSNGFTPYSYEPFERSLKRKDWYGTLNTIYGRD
                     FDWITRRVLTARKYRILNIEV"
BASE COUNT         1013 a         1153 c         1267 g         1104 t
ORIGIN      
        1 tattgacatt ggctctaaaa ccgctgaatt tgggttttca ggagcttgcg ggcaaagtga
       61 atgcggctct tgatcgtacc cagcggttcc tggagcattt ccgcaatttc gtggtatttg
      121 aacccatcaa aataaagcag gaaaggatta cggaagatct caggcagttt gtgaatggct
      181 gactgaatgt cctttacccg gagattgctc tcgacggaca ccggaaccgc catttgggcg
      241 gagttcaaca ggaactcgtt aggggtactg tcaaaaatgg tattttgctt ggccttgcgg
      301 cggtaattat taataaatat attccgcatg atggtataga gccaagcctt gatattggtc
      361 cccacattgt atttgtcctt gttggccagg gcgcggtata atgtttcctg gaaaaggtct
      421 ttggctgctt cggaatcacg ggtaagcgtg atagcgaacg gcttcaagaa ctccgcatta
      481 ttgaccaaaa gctggttgaa ttctactgta gccatgaaat ttaagatggt ttaatgatcc
      541 cgattcaaaa ttaggtatta aagaacaacg tgcccggaat atgtgacttg tggtttagtt
      601 aaaattcaaa agtcagaaaa tcagggtgtt gtgctttacc gatcgttgat gcatgtgcca
      661 tgctgccctc ctttttttca gaatatacct tcaaaaaaag ggcctgcggg ataagaatgt
      721 cagaacgaca ggaatggcgg cgacaacggc gggggagtgg cagaggcgcg gcaaaatcgg
      781 cggcggggat catcggggtg cgaagcctaa agccggggat tttggatctt accggtttgg
      841 agaaggcgat ctactgtttg gaacagatgt ctggaggaac cgagagcgtg aagatggcgg
      901 gtgtggtgaa gatcagtacc gaggaagtcg aaatagccct ttttgagcag gtattcggcc
      961 agctggcggg cgtctttgcc ataatagccg atgagggaga gcaggttcag ctggaaaaga
     1021 cagccggcat cgcagatctg gtcataccag cccttattgg cgccaaaata aagatagcgc
     1081 tcgggatggg cgaggatggg ctgatagcct ttcacctgca gttggaagag caattccttg
     1141 agattgatag caggcaccgc gaaagacagc tcgacgagga tgagattgtc ctttagcgtc
     1201 aaaaatggtc cgcccgcgtc aagggcgcgg gcgaacctat cgtccatcag gtattccgcg
     1261 gccgcatgaa actcaacgtc gatcccattc tcccgcagcg cggctgccac ggagtcgcgg
     1321 ccggcccgaa tgatgtcggg tgtattggga aaaatgtccc cgatgacgtg tggggtagtg
     1381 atgatttttt tatacccaag atcgaccagt ccgcggatga gccggagact cgtctccata
     1441 tccggagagc catcgtcgat gcccgggacc aggtgggagt ggatgtcaca gccaagaccg
     1501 gcaagcgcgc cggatggatc gggtggccgt tttttaaaca gcgagaacat gggattgata
     1561 ttcttcgtac acccggcgga gcccttcctc gagcgaaata gaggccttcc atcccaaccc
     1621 gttcagtttg gacacatcca ttaactttct tggcgtgcca tcgggtttgc tggtgtcatg
     1681 ccggatctca cctgtataac ctacgatcct tccgatcaac gcggccagct cgccgatctc
     1741 gaggtcttcg cccgtgccga tattcaccag accgggttca tcgtagccct gcatcaggaa
     1801 atagcaggcg tcggcaaggt cgtcggcatg gaggaattcg cggcggggtt tgcccgagcc
     1861 ccagatggtt acgaagggcg ccgagttcag cttagcctca tggaatttgc ggaccagcgc
     1921 cggcagcacg tgcgaggtag tgagatcgta attgtcattg gggccataga gattggtagg
     1981 catcaccgag atgaaattgc agccgtattg cgagcggtag gcatcgcata gcttgatgcc
     2041 cgcgatcttg gcaatggcat agggctcgtt ggtgtcttcg agcgtccccg tcagcaggta
     2101 ttcctctttc agcggttggg gagccatctt gggatagatg cacgaggagc cgaggaacag
     2161 cagcttcctg acgccattgc gatatgcggc gtcgatgata ttgctttcga taaggaggtt
     2221 gtcgtacagg aattccgccc ggtacgtatt gttggccatg atgccgccga cctttgcggc
     2281 cgcgaggaaa acatgatccg gtgtctcttc ggcgaaaaat tcccggactg ccgcctggtc
     2341 cctgagatcg agctcggcag aggttcgcgt cacgatattg gtgaatcctt ctttttgcag
     2401 tctgcgcgaa atggccgatc ccaccatacc ccggtgtccg gcgatgtata tcttttcttt
     2461 cgggttcata tgattattcg tactgattct tgatgtgaaa accggattct ttcaggaccc
     2521 tttccttccg gaaaagctgg acatcggcgt ctaccatctc cttcaccagc atgggcagcg
     2581 tatagacagg ggtccagccc agcttcgtat tcgccttcga ggcatcgccg atgagcaggt
     2641 ccacttccgt cggacggtaa taacgggcat ccacggccac tacttccttt ccgaccggga
     2701 cgataaaatc cgggttggag ctggaggcta ctatgccgat ctccttctct tcagaccctt
     2761 tgaactccag ttctacccca atctctgcaa aagccatgcg cacgaagtcg cggacgggag
     2821 tggtgatgcc agtagcgatg acatagtcct caggttcagg ctgttgcaag attcgccaca
     2881 tcgcctccac gtaatcacgt gcatgacccc agtcgcgccg ggcgtcgaga tttccgaggt
     2941 agatcttatc ctgcagaccc agtccgatct tggctactcc gcgggtgatc ttgcgcgtga
     3001 cgaaggtctc gccccgcagg ggggactcat gattgaataa aatgccattg gtggcataca
     3061 tcccataggc ttcgcggtag ttgaccgtta tccagtaggc gtaaagtttg gctactgcat
     3121 atggcgatcg tgggtagaac ggtgtgttct cgctctgcgg caccgcctgg acgagaccgt
     3181 acagttccga tgtggaggcc tggtagacct tcgtcttttt gaccagcccc agcaaccgca
     3241 ccgcttcgag aatgcggagg gttccgatgc cgtcggcatt ggcggtatat tcgggtgttt
     3301 caaaactgac atggacatgg ctcattgccg caaggttgta gatctcatcc ggctgtgtct
     3361 cctggatgat ccggatcaga ttggtcgaat cggtcagatc tccgtaatgc aaaaagaagt
     3421 tcacatggga ctcgtgcggg tcctggtaga gatggtctat ccgatcggta ttgaacagcg
     3481 agctgcgccg tttgatgccg tgaacgatgt atcctttctt caagagcagt tcgcttaaat
     3541 aggcgccgtc ctgcccggtg acgcccgtga tcaacgcaac ttttgacatg gtttgtttat
     3601 tatacttcaa tattcaatat tctgtacttt ctcgctgtga gcacccggcg ggttatccag
     3661 tcgaaatctc tgccatagat cgtgttcagc gtgccgtacc aatcctttct tttcaacgac
     3721 cgttcaaacg gttcatagct gtaaggcgtg aatccattgg acagcaacag ccggtggagc
     3781 tgttcttcct ggaaaccgta gcgtgagccg gagccattca gctccacgat cagcgctttc
     3841 agcccgggat ccgccaggag ccgtgacgcc ccgaggaagg ccggccattc gaaaccttcc
     3901 acatcgatct taagaaggac cggagtccgt ccttccagca tttcgtccag cgtcctgacg
     3961 ggcacttcga tgaccggccg gccattcgaa tcctccgtgt ttttcacgac gtggttgacg
     4021 gagtcctcag acgcggtgaa aagaagggtt cctgccgatg cactcgcgcc aaatgctttc
     4081 aggaccactt gttcggcaac cttgttggct gcgacattcc gctgcagcct cttgaaagtg
     4141 gaagggatgg gttcgatgga aatgacatcg gccccggcat tctttgcggc cagaatggta
     4201 tatactccca cattagcgcc gacgtcggcg aaaacgtcct cccggcgcaa gatatgcaag
     4261 aggaacgcca tatcctcgaa ttccagcaat cccgtataaa tattccccgt cgccccttcc
     4321 atgcccttct ccacgataag aatgctgtcc tcgacgaggg acatctgttg tggtccgggt
     4381 ctcatccttt gcccgaactg ccaggacaat aattgtctga aggcaagcgg gcgatttttc
     4441 gatgcaagcg gatggttcca gatgaaccgc accattttga aaaactgttt caccagaaaa
     4501 ggtttatctc gtcataaatt gggtaatttt tctcagt
//
LOCUS       DASYSV010000513         4728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1130743, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000513 DASYSV010000000
VERSION     DASYSV010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130743"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VGQ51_15905"
     CDS             <1..258
                     /locus_tag="VGQ51_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV9038120.1"
                     /translation="VLSGMAAFLTGVTRTPFTSAILVLEMTDRHNVIFHLMLAAMVAS
                     LVSMLVDKHSLYDHLKYQYMRSVLREDDPPPAYENAGAATD"
     gene            331..1590
                     /locus_tag="VGQ51_15910"
     CDS             331..1590
                     /locus_tag="VGQ51_15910"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEV9038121.1"
                     /translation="MIEPKFVVQMQLYNVLAILTVLAALFGYINYRLLRLPETIGVML
                     ISVVASLVIIVLGLIDPFFFKEVPKFIRGIDFYTVLVKIMLGFLLFAGAIHLDGHALL
                     KQSTPILTFSTISVLISTATVGLLVYFLCQLLHEPIALLYCLLFGALISPTDPIAVLS
                     ILRKAGIPASLEVKITGESLFNDGVGIVVFLCLYQVAEAGIGHLSAGQVAVLFLQQAA
                     GGILLGLALGYTGYLLLRSIDQYQVELLITLAIVMGGCALAERMNVSGPLAMVMAGLI
                     TGNKTRVHGMSEATRDYIDKFWGAIDNLFNTVLFLLIGLEMIVIPMSLPILWLALGEI
                     PIVLFARYISVQLPIGILRRHTCFEKNAVSILTWGGLRGGLSVALALSLPSSMSGGRF
                     VAMTYLIVLFSILVQGISIGKVARRLL"
     gene            1968..4193
                     /locus_tag="VGQ51_15915"
     CDS             1968..4193
                     /locus_tag="VGQ51_15915"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HEV9038122.1"
                     /translation="MIRPNRLLIVTGLLPFEFVEQRGRMVAIPTYGQIADAMSCFLRM
                     KASMSAFPEITWTGIALCNPLVWESLRDRLPDKGHDWHPIFLPVRHQETLQQGFANKV
                     LWPLFHYFPSYVEFTTVGFENYLDMNCQFANSMERFVRPGDTVWIHDYQLLPLAALLR
                     QRFPELIIGLFLHISFPSFEIFRHLPGTWQEQLLKGMLGADLIGFQTEEYATLFRNCA
                     RLTLATGGDGKTIEFENRLIKTGAFPLGIDYSRFNRPGDDLPEFRQLTAEFVQRFKDK
                     KIIYSIDVLDYTKGVLNRIEAFDYFLRENVDYLETVVLILVIVPSREHLSRYAERKKM
                     IDEAVSHLNGKFGTLGWQPVFYMYRELGFEELLTLYRLCNVALLTPLRDAMNLYAKEF
                     IASRTDAKGILILSEFDGAAEQLKEALFVNPNDVPNIGKAIKAALEMRVDEQNRRMAA
                     MRHSVSSQDVLHWGKTLLDTLEHARSEQQEFNARYFDAYSQRQLLESYRHAQKRLIFL
                     DYDGTLMPFFIQPSLAKPSDMVLETLSKLAEDERNSICIISGRDSDTLEAWLGQLPIH
                     IAAEHGARIRYRGAAWQNLVVPPAKWKEDIIPIMDHYSEQCPGSFVEQKDFSMVWHFR
                     NADPQVASKLKTDLYGVLTGISASRSFQVTMGKQIVEVRSRQMDKGTFVSNLLGREAG
                     DFILAIGDDRTDEDMFRALADKKCAYTIKVGYESSHALFNVHTPQSVISLLSTLGHLR
                     S"
     gene            4370..4618
                     /locus_tag="VGQ51_15920"
     CDS             4370..4618
                     /locus_tag="VGQ51_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038123.1"
                     /translation="MEPENSTSSELKSAIFADSQLFLANLHAGASEEQLNTILARMKE
                     RHDRLIAEYGLTLQPEFWNILQSRLANRKNKDIIDTAL"
BASE COUNT         1139 a         1179 c         1125 g         1285 t
ORIGIN      
        1 gtattatcag gcatggccgc gttcctcacc ggagtcaccc gaactccctt tacctctgcc
       61 atcctcgtgc tggaaatgac cgaccgacac aatgttatct tccatctcat gctggcggct
      121 atggtggcca gtctcgtctc tatgctggta gacaaacact ccctttacga ccatctgaaa
      181 tatcaataca tgcgctccgt tctcagggag gacgatcctc cgccagccta tgagaacgcc
      241 ggggctgcaa ctgattgacc gaccaattat caggtaacag tcaatggcct tacatgaatt
      301 tgggcagcta ccttaataag ggtaatttcc gtgatcgaac ccaaattcgt tgttcaaatg
      361 caattgtaca atgtcctcgc cattcttacc gttttggccg cactattcgg ctacatcaac
      421 taccggcttc tgcgcctgcc cgaaactatt ggagtaatgc tgatctccgt ggtggcttca
      481 ttggtcatta ttgttctcgg cttaattgat ccattctttt ttaaggaagt gcccaaattc
      541 atacgaggga ttgattttta tactgtcctt gtgaaaatca tgcttggctt tctgctgttt
      601 gcaggagcca ttcatcttga cggtcacgcc ctgttgaagc aaagtacgcc tattctgaca
      661 ttttccacga tcagtgtcct tatttccacc gcgactgtgg gattgttagt gtactttttg
      721 tgtcaattgc ttcacgagcc aatcgccttg ctgtattgcc ttcttttcgg agcgctgatc
      781 tctcccaccg accctattgc cgtcctgagc attctgcgta aggctggtat tcctgcctcg
      841 ctggaggtaa aaattaccgg agaatctctt tttaacgatg gcgttgggat cgttgtattt
      901 ctctgtcttt accaggtagc cgaagcagga atcgggcacc tgtccgcggg tcaagttgcg
      961 gtcctgtttc ttcaacaggc ggccggaggc attttacttg ggcttgcatt gggatatact
     1021 ggttatctgc tcctccgaag catcgatcaa taccaagtcg aactccttat taccctggca
     1081 atagtcatgg gcggttgcgc tttggcggaa cggatgaatg tcagtgggcc gcttgccatg
     1141 gttatggcag gactcatcac cggaaacaag acaagggttc atggtatgag cgaggctaca
     1201 agggactata tagataagtt ctggggagcg atcgacaatc tcttcaatac cgtgcttttt
     1261 ctgctgatcg gattggaaat gatcgttatt cctatgtcat taccgatact ttggctggcc
     1321 ctgggtgaaa tacccatcgt actcttcgcc agatatattt ctgtccagtt acctatcgga
     1381 atattacgaa ggcatacctg ctttgaaaaa aatgccgtca gcattctgac ttggggcggg
     1441 cttcggggtg gtttgtcggt ggcccttgcg ctgtctttac catcctcaat gtccggcggg
     1501 cgcttcgtgg cgatgaccta tctgatcgtt ttattttcga ttctggtaca gggcatcagt
     1561 atcggaaagg ttgccaggcg gctcctttga agcatcggat atatactgtt tgtcaacatc
     1621 cagctccagg ttttagtcgt ctcataatac cccggttcat acgcccttgc ataccaaaag
     1681 ctataggcgt cctgtgcaga aacaccatcc acgccattcg ctttgagcgt cctggccaat
     1741 tgctcctcac cgatgcggcc agccgcactg tatttggcag gcgttatcat caggacaatg
     1801 attttctgga acttcctgtg atttacattg gcgccgccca cgaggaggat agttgcaagc
     1861 tcggacctga tcgcttcagg aattctaatt gaattctaac ttctttttaa tgccaatctt
     1921 ggcaactctg ggtagcttta caaacgatta actaaagcaa tcatactatg atccgtccca
     1981 acaggcttct gattgtgacg ggacttctgc cattcgagtt cgtagagcaa cgcggtcgaa
     2041 tggtggccat accaacctac ggccagatcg cagacgctat gtcgtgtttt cttcgaatga
     2101 aggccagtat gtctgcgttc ccggagatca cctggaccgg aatcgcgtta tgtaatcctt
     2161 tggtatggga gtccctgcga gaccggctgc ccgacaaagg tcatgactgg catcctattt
     2221 tcctgccagt cagacaccag gaaactctgc aacagggatt tgccaacaag gtattgtggc
     2281 cgttgtttca ttattttccc tcttatgtgg aattcactac tgtgggtttt gaaaattacc
     2341 tggacatgaa ctgccagttc gccaatagca tggagcgctt tgtcaggccg ggcgataccg
     2401 tatggataca tgactatcaa ttattgcctt tagcggcatt gttaagacag cgatttccgg
     2461 aacttatcat cggtcttttc ctgcatatat cgtttccttc attcgagatc tttcgacatc
     2521 tgccgggaac ctggcaggag caactcctga aaggcatgct tggcgccgat ctgatcggct
     2581 ttcaaaccga ggaatacgca actcttttcc gaaattgcgc ccggttgacc cttgctacag
     2641 gcggagacgg caaaaccatc gaattcgaaa acaggcttat caaaaccggg gcctttccat
     2701 tgggcatcga ctactccagg ttcaatcgtc ctggcgatga tctacctgag ttccggcaac
     2761 taaccgctga atttgtccaa aggtttaagg acaagaagat catttactcc atcgacgtac
     2821 tcgattatac aaagggcgtg ctgaatcgga tcgaagcatt cgactatttc ctgcgggaaa
     2881 atgtcgatta tctggagact gtcgtcctga ttctcgtcat tgtaccatcg cgtgagcacc
     2941 tctcccggta tgctgaacgc aagaaaatga tcgatgaggc cgtcagtcac ctgaacggga
     3001 aattcggtac actcggctgg caacctgtgt tttatatgta tagggagttg gggtttgaag
     3061 agctgttgac gctttaccga ctgtgcaatg tggcgttatt gacgccgctt agagacgcca
     3121 tgaatctgta tgccaaggag tttatcgcca gccgcacgga cgcaaaagga atactgatct
     3181 taagtgagtt cgatggcgcc gccgaacaac ttaaagaagc gttattcgtc aacccaaatg
     3241 acgtcccaaa tatcggaaag gccataaagg cggcattaga gatgcgggtg gatgaacaaa
     3301 atagaagaat ggcagcaatg cgccattccg ttagcagcca agacgtattg cattggggaa
     3361 aaactttatt ggacaccttg gaacatgccc ggtcggaaca gcaggaattc aacgcgcgct
     3421 attttgacgc gtattcccaa cgacaactgc tggaaagcta ccggcatgct caaaagcgac
     3481 ttatcttcct ggactacgac ggcacactga tgcccttctt tatccagcca tcgctggcaa
     3541 aacccagcga tatggtgctt gaaacactca gcaaattggc tgaggacgag cgcaattcca
     3601 tctgcatcat cagcggccgc gacagcgaca cgcttgaggc ctggttggga caacttccca
     3661 tccatatcgc cgccgaacac ggtgcccgga tccgataccg tggggccgcc tggcaaaacc
     3721 tcgtcgtacc cccggcaaaa tggaaagagg acatcatccc cataatggat cattattccg
     3781 agcagtgtcc gggttccttt gtggaacaaa aagacttctc gatggtctgg cacttccgga
     3841 atgcggatcc tcaagtcgca agtaagttaa aaacggatct ttatggtgtc ctgactggga
     3901 taagcgcgag ccgatcattc caggtgacca tgggcaaaca aattgtcgaa gtacgcagca
     3961 gacaaatgga taaaggtact ttcgtttcca atctgctcgg tcgggaggcg ggcgatttta
     4021 tactcgccat aggtgacgat cgtacggatg aagatatgtt ccgcgccctg gccgacaaaa
     4081 aatgcgcgta tactataaaa gtcggctatg agtcgtccca tgccctgttc aacgtacata
     4141 ctcctcaatc ggtgatttct ctattaagta cacttggaca tcttaggtcc taacgcgctg
     4201 gcattgattt ttctgtttgt atggaaatag acgagcagtc tcagtgtcat atcgctcgta
     4261 ttaacccggc tcttttcgcg acccagggcc cgggttttta gcggtatata ctttacgatt
     4321 ttggtgcaat ttttgcaata tgcacaggat tagttacgta taacacaaca tggaacctga
     4381 aaattcaact tccagcgaac taaaatccgc gatttttgca gattctcaat tatttttagc
     4441 aaatcttcat gcgggtgctt ccgaggagca attgaacacc atcctcgccc gaatgaagga
     4501 aaggcacgac cgccttatcg cagaatatgg gcttacgctt cagccggaat tttggaacat
     4561 attgcagagt cgtctggcaa accggaaaaa caaggacatt atcgatactg ccctttgata
     4621 gccaccgcat taccaacttt tgtcctcctt tagaaacgct gtcatttgga acatagaccc
     4681 tatcgaatat tattatgtga tgatgatccg gatgaggtcc tatttctc
//
LOCUS       DASYSV010000514        15313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1002706, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000514 DASYSV010000000
VERSION     DASYSV010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002706"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VGQ51_15925"
     CDS             <1..582
                     /locus_tag="VGQ51_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEV9038124.1"
                     /translation="NLEKLMLKADHLGQDYVINKNIKGILSGQIKSYIQVHPDLTPLI
                     DQSQAQMDIEIIDGELVNFAPMQAMASYFGGKNLTRIRFDTLRNSLALKNGVLSIPDM
                     NINSSLGFMEISGTQSIDTHMEYYIRIPLKLVTSAGWHKLFGKKQEEVDPDQVDAIEY
                     RDKDKRVHFINLRISGTPDDYKVALGKVKKAQG"
     gene            complement(622..1152)
                     /locus_tag="VGQ51_15930"
     CDS             complement(622..1152)
                     /locus_tag="VGQ51_15930"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV9038125.1"
                     /translation="MQISAILLTGLVLVGFVETWKADPSKAKVQFSVHGPFGTVHGSF
                     TGLKASIQFDEQHPGSGSISATIDPNTVSTGIGMRNHDLKHEEKWLNTAKYPTISFRS
                     THIERTSKGFEAAGDLTLKGVTKPVRIPFTFSPSGNSGLFKGEFVIHREDFGVGGKGG
                     SVGNDITISLEVPVTK"
     gene            complement(1167..2552)
                     /locus_tag="VGQ51_15935"
     CDS             complement(1167..2552)
                     /locus_tag="VGQ51_15935"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HEV9038126.1"
                     /translation="MRKILTAWLCLCVPVCLSAQYNFGIGNGNYSGIQGARINPAVTA
                     GSPLKWEVNLVSGDMLYDNTFVYIPKGSVPVLGFKSIYKGIIDNNHFYTHYDPSNPGK
                     LYQFTMSTEVLGPSVRFNIGTDQSIGLTIAARSYENMHDLPGTTAQTAFAYLMQSDLW
                     NKPLRDHSSRLNGMGWLQYGLNYSAVLLDDGTNKLKAGIGINYLQGVIAAYAKNTNLN
                     YSINDTANLQFTNSSVDYGRTDYDSYRRIGSYGDLNHGHGLSVDAGIVFSHKGDPNDP
                     TVPYDYRLGVSVMDLGSIDFNRNAAAFHLQTDTANFSAWRQVHLTSNIAVDKTLSAVF
                     YHGDSTKSRTADHFTMAMPAAFSFQGDFRLTTRLFVNATIVKGLHHGDKPGVEQPDLY
                     SVTPRLETPWWEVSVPLSMLYYGEWRPRIGLAFRVGWFFIGGDAPASLFGLGAMEGAD
                     VYAGIRFFNLK"
     gene            complement(2676..3788)
                     /locus_tag="VGQ51_15940"
     CDS             complement(2676..3788)
                     /locus_tag="VGQ51_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9038127.1"
                     /translation="MQSRRDFLYKMTGSVAALSVPASLVQACSSLIPSHDSPPLRVAI
                     MGLGSYGTRVAEAMQSCTRATLVGVISGTPSKIRAWQSKYHIPEKNCYDYTSYDAIRD
                     NPDIDAVYVITPNALHHDAVIRVAKAGKHAICEKPMAVNAKEGQEMVDACKKAGVRLL
                     VGYRMHFEPHTLEIIRMRKAGQFGKVLFFQGLSGFTIGDPTQWRLNKALSGGGSMMDI
                     GIYSVNGARYMIGEDPIWVTAQATKTDPVKFKEGVDETIQFQLGFPGGAVASCLSTYS
                     MNNLDRFFLNGTGGFAELLPATGYGPIQGRTNRGPLDQPVVTHQTVQMDAMSGIILHG
                     EQPVVPVDGEEAVRDLKILDAIYQATRTGEKVALQL"
     gene            complement(3796..4557)
                     /locus_tag="VGQ51_15945"
     CDS             complement(3796..4557)
                     /locus_tag="VGQ51_15945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEV9038128.1"
                     /translation="MNPFVKPGSIVRTIWGRGDTILLIFAGASAEFALNKSVDWLYFT
                     GRLPKDPLGRLFSTVSYARRIVFADTADANAAIDHITAIHKGVEKARGYTIPAGAYLD
                     VLFMLVGYSISAYETVERKMSAGEKEEVFSVFLRVGRRMGLSGLPASYTEFMALRETH
                     LRENLVRSEFSKDLYRQYRKHLGAVRYFVLLQAQVLLVPDRVRRLLRLSSIPFFYPVV
                     QLYKAARMVRLDKSIKYLLLPPQYREQIFGLDSLT"
     gene            complement(4561..4959)
                     /locus_tag="VGQ51_15950"
     CDS             complement(4561..4959)
                     /locus_tag="VGQ51_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HEV9038129.1"
                     /translation="MLRFNRIYFLLTVVLIGVESFIGLCMHDPIIRPYVGDVLIVVLL
                     YCLIRSWWDLPVVPLALGVLVFAYGEETLQYFHFADRLGFTRPSLMRTLIGTWFSWVD
                     MGCYTIGIGMVLAAEMFFRQRHWKLAETKS"
     gene            5090..5986
                     /locus_tag="VGQ51_15955"
     CDS             5090..5986
                     /locus_tag="VGQ51_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV9038130.1"
                     /translation="MERTEICCIGHITHDKVVTPRSVVHMAGGTAFYFSNALSKLDVR
                     YRLVTALAQADMSFVNDLRSKGIDVTVLPSAETVFFENRYSENQDHRVQRVLGRADSF
                     QPGSLKDIDARVFHLGPLLAGDIPLGVFPALARKGQISLDAQGYLREVKRSNVLAIDW
                     ADKKEALQYVHTLKVNESEMTVLTGIADVRQGAKELARWGVQEVVVTLGSKGSVIYND
                     GVLTQIPAYIPTTSVVDATGCGDTYMAGYLYQRVRGVSIREAGEFAAAMATLKIEASG
                     PFKGTSAEVLTLLAMRKDRVFR"
     gene            6007..6429
                     /gene="paaI"
                     /locus_tag="VGQ51_15960"
     CDS             6007..6429
                     /gene="paaI"
                     /locus_tag="VGQ51_15960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEV9038131.1"
                     /translation="MEEKGKTPAAITALMLERDRFSHWLGLKIDEVGPGYCRLHYRIN
                     EQMVNGFNQAHGGVLFSAADSAFAFACNSHGVITVALDVSITFTRPARTGELLFVEAK
                     EIHLGNKTGVYDIRTTNTAGELVALFKGTAYRTSHPVK"
     gene            6624..7967
                     /locus_tag="VGQ51_15965"
     CDS             6624..7967
                     /locus_tag="VGQ51_15965"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9038132.1"
                     /translation="MKRFIIGTLTLSMLSIVAFSQPSDRADSTLTLTQCVDYALKHQP
                     FINQAVVNIAIARTSNSIAMAGWLPQVNLSANLTHYLTLPTNFIKNSTTGTVSEQKTG
                     VVNSFIPVLGVTQTIFNPALLYAAKSAPLYVKEAEQITDSTKIGIVAGVSKTFYSLLL
                     TLEQINVLKEDTARLTKNMSDTYHQYVSGITDETNYDEAVISLNNSKFQLRQAVENVL
                     PQYAALKQVMGYPPLSNFNVVYDTTRMMEDISFDTTQQLQFEKRIEFQTLATVKALQQ
                     RQIAYYQKAWLPTVGAFFDYDYVFQNNSFGQLFSQAYPYSFLGLSVTMPVFTGLSRIN
                     NIKRSRLQADLLDYSEFSLKSQIYSEYTTALANYKSNLYSLSISKDNVALARRTYDIV
                     QLQYLQGVVPYLNVITAESNLITSELTYETSLFQILSSKIDLEKAMGIITINKPN"
     gene            7984..9153
                     /locus_tag="VGQ51_15970"
     CDS             7984..9153
                     /locus_tag="VGQ51_15970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9038133.1"
                     /translation="MNRLASILVFVSSLMIFSCKPKPTPPTPPVAVNLMTVSERSVTY
                     YDKYPSTTVAISQVSLTAQVTGYVTSINFTEGSHVHKGQLLYKLDERLYTAAVDQARA
                     ALRMDSGNLKLVQQDADRYAYLAKYKAIATQLVDHANVALQNAKDSVFAAQQLLKTAL
                     TNLSYSSIYAPFDGTIGISQVRVGNLVSPGSTVLNTVSTDNPMGVDFLVNEKQLPHFE
                     QLQAGKARSIDSLFTIQLPNGELYRFPGKILFIDRAVDPQTGSIDIRLAFPNPDYILR
                     PGMSTVVRVHNQDASPQTVVPGRAVVEQMGEYFVFVAKDTVMRSKTADSATAKDADTA
                     RTPELRAFQKKVELGETVGPNVVIKSGVNAGDRIVLDGVQAIHEGSRINAGPAKH"
     gene            9168..12449
                     /locus_tag="VGQ51_15975"
     CDS             9168..12449
                     /locus_tag="VGQ51_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9038134.1"
                     /translation="MIANTFIKRPVTAMVVSIVLVIAGAICILTLPIDQYPDITPPVV
                     QVNAQFTGADAQTVEQTVATPIEEQVNGSPGMEYMQSNSTNNGLLSMNVTFGIGTNVD
                     IATLDVQNRVSIANPLLPAVASRLGVTVRAINPSQLMMIAVYAPQGTHDITFLDNYTN
                     IFIQDALLRVPGVGSISRSTDDFSMRVWMDPQKMASHNLMPQDIISALNSQNVQVAAG
                     SAGVPPQSRAQAFEIGILVNGRLSKVSEFENIIVKNNPARGELVYLKDVARVELGKFT
                     FSSNSFVDGHRASILRIYQAPGSNALETAGGIYKKLALLRQSFPADVEYSIPFEAVTV
                     VKVSMADVVRTLFLTLGLVVLVVFVFLQSFRSTLIPILAIPVSIVGTFIFFYPLNFTI
                     NTLTMFGFVLAIGIVVDDAIIVVEAVQHYMDEEGMSPKEATYVAMKDISAPVVAIALI
                     LAAVFVPVGFIPGIVGRLYQQFAITIAISVILSAFIALSLTPALCTLLLKPTDPNKKK
                     RGLSKGFEHFNHWFDRLTQRYKNGVDRSITGFRWIIVLLVVICIGAWFFFLKKPTGFI
                     PSEDDGNLYVTFQLPPASSTAQSVDVMNRLMDVVSNTPGVAHYAALSGLNVVNNASNS
                     NAGTIYIQLRPWDERDKKSEQVPGIIGVLNKRIADAGIRNANAEVIPPSPIPGVGETV
                     GFSMQIEQRSTSDDLQAFQRVVNKFVAEVNRNPAITGAYSFYSANTPGYNLKVDREKC
                     EKLGVNVGDVFTTIQAFMGSLYINDFTEYNRTFHVVVQADTNYRALITDMNKYYVRNQ
                     DSTMVPLSTLCTYTPAETAPLIQHFNIFRSAEIDGGPAAGFSSNQATDSLIAIANRVL
                     PEGYNYEFSGLSYEEIKAGSTTIYIFLFSITFVFLFLAALYESWSVPFSVLLAVPVGA
                     FGAIFTLFLVPSLTDNVYAQIGLITLIGLAAKNAILIVEFAKVRVDRGEELIQSTLEA
                     VKLRLRPIVMTSLAFILGVLPLVLASGAGAVARRTIGYTVLGGMTAASTIAIFIVPVL
                     YVVITRMSYGKERLAWLRAHHEELMEKSRKVEAQNIDPELEFELARSRKQNSQQGDEN
                     KPQQG"
     gene            12446..>15313
                     /locus_tag="VGQ51_15980"
     CDS             12446..>15313
                     /locus_tag="VGQ51_15980"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9038135.1"
                     /translation="MIANTFIKRPVTAIVISLVLVISGVISIFNLAVDQYPDISPPSV
                     SVRGSYTGADAETVEQTVAIPVEEQVNGTPGMEYMQSTSTNSGGVNVRVTFRVGTNVK
                     VAALNVQNREGIASPMLPSVVSKLGMTVRASNPDQLLLVAIYSPKGSHNITFLDNYTS
                     VFIQDAILRVPGVGDVQARTDQFAMRIWMDPQKMASYHVTPSDVSAAVNAQNQYVAAG
                     SAGAPPQHISQSYELNILVNGMLNKPEDYEKIVVKTNPKTGAMVYLKDVARVELGKFT
                     FSSNAFVDGKRCSLLMIYQAPGSNALETADAVYAKLAELKKTFPNDVDYKIPFEAITI
                     IRVSMDEVEKTLLQALALVALVVLLFLQNWRSTIIPIFAIPVSILGTFCFFIPLGFTI
                     NTLTMFGFVLAIGIVVDDAIIVVEAVQHYIDEQHMSAKEATYQAMKEISAPVVAIALI
                     LAAVFVPVGFIPGIVGRLYQQFAITIAISVILSAFIALSLTPALCTLLLKPHNPHKKK
                     NWVDKFFASFEKGFSAFTYRYVHGVRRSIKYSGITVLVLAGICVGAFYLFRTKPSGFV
                     PSEDGGRVYITFQLADASATTQSVDVMQKMMKVVNSTPGILHYVAVSGFNILNGGATS
                     NAGSFFVMLTPWDQRTTPQTQWPGISTELYKRFARAGIKNASIHVIQPAPIRGIGISA
                     GFTFEIEQGSSTDDIYTFEKAVNRFVAEAKKNPSTSTAVCYFSAHTPSYDLTVDREKC
                     QKMGVNISQVFQTMQAFMGSLFIGNFTLYDRTFHVVMQADTAYRALIGDMDKYYVRNS
                     RDSMVPLSTLISYKPSQAAPLITHFNIFRSAEVQGSIPPGYSSGQSIDDLRKLAAKYL
                     PRGYTYDFSGLSYEEIKAGSMTVYIFLFCIIFVFLFLAALYESWSIPLSVMLAVPISA
                     FGAILALWLAPSLTDNVYAQIGLITLIGLSAKNAILIVEFAK"
BASE COUNT         3535 a         4248 c         4086 g         3444 t
ORIGIN      
        1 aatctcgaga aactgatgct taaggcggat catctcggcc aggattatgt catcaacaag
       61 aacatcaagg gtatcctgag cggtcagatc aaaagctata tccaggtaca tcccgatctc
      121 accccgttga tcgatcagtc acaggcgcaa atggatatcg agatcatcga tggagaattg
      181 gtgaatttcg cccccatgca agccatggct tcgtatttcg gaggaaagaa cctgacccgg
      241 atccggtttg acaccctgcg taattccctg gcgctgaaga acggcgtttt gtcgataccg
      301 gacatgaaca tcaattcttc gctgggattc atggagatct ccggcacgca atcgatagat
      361 acgcacatgg aatattatat ccgcataccg ctgaagctcg tgacctcggc gggctggcac
      421 aagctgttcg gtaaaaaaca ggaggaagtc gatcccgacc aggtggatgc catcgaatac
      481 cgggacaagg acaaacgggt ccacttcatc aatctgagga taagcggcac ccccgatgat
      541 tataaggtgg cgttggggaa ggtgaaaaaa gcgcagggct gatcctgcat gtcttaccgc
      601 agagtgcccc cgtcggccac gctatttcgt aacagggacc tccaggctga tcgtgatatc
      661 gtttcctact gaccctcctt tgcccccgac gccgaaatct tcgcgatgga tgacgaattc
      721 gcctttgaac aacccgctgt tgccgctcgg actaaaggta aagggtatac gcaccggttt
      781 ggtgacccct ttcagcgtca gatcgcccgc tgcttcaaag cctttggatg tgcgctcgat
      841 atgagtggag cgaaaagaga tggtgggata tttggccgtg ttcagccatt tttcctcgtg
      901 tttcagatcg tggttccgca tgccgatgcc cgtgctgacg gtattggggt cgatggtggc
      961 ggatatcgag ccgctcccgg gatgctgttc atcgaattga atagaggctt ttagtcccgt
     1021 aaagctcccg tggacggtgc cgaacgggcc gtgaacggag aactgcacct ttgccttcga
     1081 aggatccgcc ttccaggttt caacaaaacc caccagtaca agcccggtaa gcaggatagc
     1141 gcttatttgc atagtctgtc tattttttac ttcaggttga agaaccggat gcctgcatag
     1201 acgtccgctc cctccatggc accaagccca aacagggatg ccggtgcatc gcctccgata
     1261 aagaaccagc cgacgcgaaa cgcaagaccg atccttggtc tccactcccc ataatacaac
     1321 atcgacagcg gcacgctcac ctcccaccac ggagtctcca gtcttggcgt aacagaatag
     1381 agatcgggct gttccacgcc gggtttatcg ccgtgatgca ggcccttgac gatggttgcg
     1441 ttcacgaaca accgggtcgt gagccggaaa tcgccctgga agctgaacgc ggcaggcatc
     1501 gccatggtga aatgatcggc ggtcctggac ttcgtcgaat caccatgata gaaaactgcg
     1561 ctgagtgtct tgtcgaccgc gatattgctg gtcagatgca cctgtctcca ggcggaaaaa
     1621 ttggctgtat cggtctgcag atgaaaggcc gccgcattcc ggttgaaatc gatggaccca
     1681 aggtccataa cggaaacccc caaccggtaa tcatagggca ccgtcggatc gttggggtca
     1741 cctttatgcg agaaaacgat acctgcgtcg acactgagac catgtccgtg gttcagatcg
     1801 ccatagctgc cgatcctgcg ataagagtca taatccgtcc gcccatagtc gacgctggaa
     1861 ttcgtgaatt gcaggttggc agtgtcattg atggaataat tgagattggt atttttcgca
     1921 tatgccgcaa tgactccctg caaataattg atgccgatgc ccgctttcag cttgttggtc
     1981 ccgtcgtcga gcaggacggc ggagtaattg agcccgtatt gcagccatcc catgccattt
     2041 agccgcgagc tgtgatctct gagcggtttg ttccatagat cggattgcat caggtaggcg
     2101 aaggctgtct gtgcggtggt tccggggaga tcgtgcatgt tctcgtacga gcgggcggca
     2161 atcgtcagtc cgatagattg gtcggtcccg atattgaaac gaaccgatgg gccaagcacc
     2221 tcggtcgaca tcgtaaactg atacagctta cccgggttcg acggatcgta atgggtgtag
     2281 aaatggttgt tatcgatgat gcctttgtag atggatttga agccaaggac gggtaccgag
     2341 ccttttggaa tatagacgaa cgtgttgtca taaagcatgt cacccgaaac cagattgacc
     2401 tcccatttca ggggacttcc cgccgtcacg gcgggattga tgcgagcgcc ctgaatgccg
     2461 gagtaattgc cgttgccgat gccaaaattg tattgggcgc tcagacagac aggcacacac
     2521 agacacagcc acgcggtaag tatcttgcgc atacaataga tttgcgatct tccttgaaga
     2581 ccttgttaca ggcagtcgaa tgagggaagg aaaatgaacg ctggctggca atagaatatc
     2641 accagtatca acgcgtcgat cgggcggatg gttgcctata attgcaacgc gaccttttca
     2701 ccggtgcggg tcgcctgata aatggcgtcg aggatcttca gatccctgac cgcctcttcg
     2761 ccgtccacgg gtacgaccgg ttgctcgcca tgcaggatga tgccggacat ggcgtccatc
     2821 tgtaccgttt ggtgtgttac gacgggctga tccagcggtc ccctgttggt gcgtccctgg
     2881 atgggtccgt aacccgtggc aggcagcagc tccgcaaacc cgccggtgcc gttgagaaaa
     2941 aagcggtcta aattgttcat actgtacgtg gaaagacagg aggcgaccgc tccgccggga
     3001 aaacccagct ggaactgaat ggtctcatcc accccttcct tgaatttcac gggatcggtc
     3061 ttggtggcct gtgccgtcac ccagatcggg tcttccccga tcatatagcg ggcgccattg
     3121 acggaataga taccgatgtc catcatcgag cctcctccgg acagggcctt attcagccgc
     3181 cattgcgtgg ggtcccctat cgtgaaaccg gagagaccct ggaagaacag gaccttgccg
     3241 aattgcccgg cctttcgcat gcggatgatc tcgagcgtat gcggttcgaa atgcatccgg
     3301 tagccgacca gcagcctgac gccagccttt ttgcaggcgt ctaccatttc ctgaccttct
     3361 ttggcattca cggccatcgg cttttcgcaa atagcgtgtt tgccggcctt cgccacccgt
     3421 atgacggcgt catgatgaag cgcgttgggc gtgatgacgt acaccgcatc gatatcgggg
     3481 ttgtcacgga ttgcatcgta actcgtatag tcgtaacaat tcttttccgg gatatggtat
     3541 ttggactgcc atgccctgat cttggaaggc gtgccgctga tgacgcccac cagcgtggcc
     3601 cgtgtacaac tttgcatggc ttccgccacg cgggtaccat aactgcccag ccccatgatg
     3661 gctaccctaa gaggtggact atcgtgagaa ggtatcaatg aagaacaagc ctgcaccagg
     3721 cttgcaggca ccgagagcgc agcgaccgaa ccggtcattt tataaaggaa atcgcgacgg
     3781 gattgcatag ttcccctaag ttaatgaatc cagaccgaaa atctgttccc gatactgcgg
     3841 cggcaacaac agatacttga tactcttgtc cagccttacc atccgggcgg ctttatacaa
     3901 ctgcaccacg ggatagaaaa agggaatgga ggacagacgc agcaatcgtc ggacccgatc
     3961 cgggaccagc aacacctgcg cctgcaacag gacgaaatag cgcaccgccc cgaggtgctt
     4021 ccggtattgg cgatagagat ctttggagaa ttcgctgcgg accaggtttt cgcgcaaatg
     4081 ggtttcccgc agtgccatga attccgtata gctggcgggc aggccgctca gacccatccg
     4141 ccggccgaca cgcaaaaaga ccgaaaagac ttcctccttt tccccggccg acattttcct
     4201 ttccaccgtt tcatacgcgc tgatcgaata gccgacaagc atgaacagga catccaggta
     4261 ggcgccggcc ggtatggtgt agccgcgggc tttttccacg cccttatgga ttgccgtgat
     4321 gtgatcgatg gcggcattgg cgtccgcggt atcggcgaaa acgatccgcc gcgcataact
     4381 caccgtggag aaaagacggc ccaggggatc tttgggcaaa cgccccgtga aatagagcca
     4441 gtcgacggat ttgttcagtg cgaattccgc cgatgcgcct gcaaagatca gcaggatggt
     4501 atccccgcgt ccccaaatgg tcctgacgat ggaccccggt tttacaaaag ggttcatagc
     4561 ttacgatttt gtttcggcca gcttccaatg tctctgtcgg aaaaacattt ccgccgcaag
     4621 gaccattccg atgccgatgg tgtaacaccc catatccacc cagctgaacc aggtgccgat
     4681 cagcgtccgc atcaacgacg gccgggtaaa acccaaccgg tcggcaaaat ggaaatattg
     4741 cagcgtttct tccccgtagg cgaagacgag tacacccagc gccagcggaa cgacgggcag
     4801 gtcccaccaa ctgcggatca gacaataaag aaggacaacg atcaggacat ctccaacgta
     4861 gggcctgata atggggtcgt gcatgcagag accgatgaaa ctctctacgc cgatcagtac
     4921 gacggtcaac agaaaataga tgcgattgaa ccggagcatt ttattaaaaa gtgctttgag
     4981 atacaaagta aagaaatccc tgtgacaatt gcaagtaaat cacatcaaat agctaatcgc
     5041 accatgttaa aaaatcacta cctttacaaa ttgcccgaaa atcccctgta tggaaaggac
     5101 agaaatctgc tgcattggtc atattaccca cgacaaggtt gttaccccga gatcggttgt
     5161 tcacatggcg ggagggaccg ctttttattt ttccaacgct ttgtcgaagc tggacgtcag
     5221 gtaccgtctt gtcaccgcac tcgcgcaggc cgacatgtct ttcgttaatg acctgcgttc
     5281 aaaaggcatc gatgtcaccg tcctgcccag cgcggaaacg gtcttcttcg agaacaggta
     5341 ttccgaaaac caggatcatc gtgttcagcg tgttctgggg cgggcggata gctttcagcc
     5401 cgggtccctg aaagatatcg atgccagggt ttttcatctc ggtcccctgc tggccggtga
     5461 tataccactg ggagtattcc ccgcactggc aaggaagggg cagatctcgc tggatgcgca
     5521 gggatatttg cgcgaggtaa aaaggtccaa tgtgctggcc atcgactggg ccgataaaaa
     5581 ggaagcgctc caatatgtac atacgctgaa agtgaacgaa tcggaaatga ccgtgcttac
     5641 agggattgcc gatgtccgcc agggagcgaa ggagcttgcc cgctggggcg ttcaggaggt
     5701 agtggtcacc ctcggaagca agggctcagt catttacaac gacggtgttc ttacccaaat
     5761 cccggcctat attccgacca cttccgtcgt ggatgcaaca ggctgcggag atacctatat
     5821 ggcgggctat ctttaccaac gggtccgggg agtgtccatc cgcgaggccg gagagtttgc
     5881 ggccgccatg gctaccctga agatcgaagc ctcgggaccg ttcaagggca ccagcgccga
     5941 agtgcttacc ctgctggcca tgcggaaaga cagggtcttc cgctagacac accgaaaaat
     6001 ctgcttatgg aagaaaaagg caagactccc gccgctatta ccgcattgat gctggaaaga
     6061 gaccggttct cacactggct gggtctgaag atcgatgagg tcggtcccgg ttactgccgg
     6121 ctgcattacc ggatcaatga acagatggtc aacggcttca accaggcaca tggcggagtc
     6181 cttttttcgg ccgccgattc tgcctttgcc ttcgcctgta attcacacgg tgtcatcact
     6241 gtagcactcg acgtctctat cacctttacc cggccggcca ggacaggcga gctcctgttc
     6301 gtcgaggcca aagagatcca tctcgggaat aagacaggag tttacgatat ccgcaccacc
     6361 aacaccgcgg gtgagctggt agcccttttc aagggcacag cgtatcggac ttcccaccct
     6421 gtcaaatgat cgcatttaat tgatttttaa ttcatcttta ccccaaaaag agtaaccttt
     6481 gcggactcct cactgaccag cactctgggt cgttccttcc ttcggataca gcagtttggc
     6541 accgcgattg ctgcgcccca tggaggagaa ctttgtcttt gtaaattttc ccgatcgata
     6601 tgcttaaaca tgttgtacta catttgaaac gtttcataat cggtaccctg accctgagta
     6661 tgctatcgat cgtggccttt tcgcaaccgt ccgatcgcgc ggacagcacg ctgacgctta
     6721 cccaatgcgt cgattatgcc ctgaaacatc agcctttcat caaccaggcc gtggtcaaca
     6781 tagccatcgc ccgcacgagc aattccatag cgatggccgg atggcttccc caggtgaacc
     6841 tttccgccaa tctcacccat tatctaaccc tgcccaccaa ttttatcaaa aacagcacta
     6901 ccggtactgt cagcgaacag aaaacggggg tcgtcaacag cttcataccc gtactcggcg
     6961 taacccagac cattttcaat cccgcgttgc tgtatgctgc aaaaagcgct cccctgtacg
     7021 tcaaagaagc cgagcagatc accgacagca cgaagatcgg gatcgtcgcg ggggtcagta
     7081 agacgttcta cagccttctg cttacactgg aacagatcaa tgtcctcaaa gaggatacag
     7141 cccggctgac aaagaacatg agcgatacct atcatcaata tgtcagcggg atcaccgacg
     7201 agaccaatta tgacgaggcc gttatttcat tgaataattc gaaattccag ctcagacagg
     7261 ccgtcgaaaa tgtccttccc cagtatgccg cgttgaaaca ggtgatgggc tatccgccgc
     7321 tatccaattt caatgtcgtc tacgatacta cgaggatgat ggaagacatc agtttcgata
     7381 cgacccaaca gctgcagttc gaaaagcgca tcgaattcca gaccctggcg acggtcaaag
     7441 cactccaaca gcggcagatc gcctattatc aaaaggcctg gttgcctacg gtaggcgcct
     7501 ttttcgatta tgactatgta tttcaaaaca acagcttcgg gcagctgttt tcacaggctt
     7561 acccctattc ttttctgggt ctctcggtga caatgcccgt ttttaccgga ttgtcccgaa
     7621 tcaataacat caaaaggtcc cgattgcagg ccgatctgct ggattacagc gagttcagtc
     7681 ttaagtcgca gatatacagc gaatacacga cggcgctggc gaactacaag agcaatctgt
     7741 acagcctttc gatctcgaaa gacaatgtgg cgctggcgag aaggacctat gacatcgtac
     7801 aactgcaata cctgcagggc gtggtgccgt atctgaacgt gatcaccgcg gaatccaacc
     7861 tgatcacatc cgaactgacg tatgaaacat ccctcttcca gatcctgtcc agcaagatcg
     7921 atctggagaa ggccatgggg atcatcacca ttaacaagcc taattaatat aacaaccact
     7981 tgtatgaata gactggcatc gatcctggtt tttgttagca gccttatgat cttttcctgc
     8041 aagccaaaac cgacgccgcc cacccctccc gtcgctgtca acctgatgac ggtgagcgag
     8101 cgatccgtga cctattatga taaatatccg tcgaccacgg tcgcgatcag ccaggttagc
     8161 ctcacagcgc aggtgaccgg gtatgtgact tccatcaact tcacggaagg ttcgcatgtg
     8221 cataagggac agttacttta caagctggac gagcgtctct atacggcggc cgtcgaccag
     8281 gccagggccg cgctgcgcat ggattcgggc aacctgaagc tcgtacagca ggacgccgac
     8341 aggtatgcct acctggcgaa atacaaggcc atcgccactc agttagtaga tcacgcaaat
     8401 gttgcccttc agaacgcgaa ggattctgta ttcgccgctc agcagctcct gaaaacggcg
     8461 ctgaccaatc tttcctattc cagcatctat gctcccttcg atggcacaat cggtatttcg
     8521 caggtgaggg taggcaatct cgtgagtccg ggttccaccg ttctcaatac ggtttcgacc
     8581 gacaatccca tgggcgtcga tttcctggtg aatgaaaagc aactgccgca tttcgaacaa
     8641 ctgcaggccg ggaaggccag atcgatcgac tcgcttttta cgatccaact gcccaacggc
     8701 gagctctatc gcttccccgg aaagatcctg ttcatcgacc gggccgtgga cccgcagacg
     8761 ggttccatcg acatccgcct ggcattcccc aatcccgact atatcctgcg tcccggaatg
     8821 agtacggtgg taagggtaca caaccaggac gcttctccgc agaccgtcgt gccaggcagg
     8881 gccgtggtgg aacaaatggg agaatatttc gtgttcgtag ctaaagacac ggtgatgcgt
     8941 tcgaagacgg ccgattccgc gacagcgaaa gacgccgata cggcccggac cccggagctg
     9001 cgcgcgttcc agaaaaaggt ggagctcgga gagaccgtag gccccaatgt cgtgatcaaa
     9061 tccggggtca atgccggcga ccgcatcgtt ctggacggag tacaggcgat ccacgaaggc
     9121 tccaggatca atgcgggccc ggcgaaacat taatttcatt tcctcgtatg attgcgaata
     9181 catttatcaa gcgtccggta actgcgatgg tcgtctccat cgtgttggtg atcgccggcg
     9241 ctatttgcat cctgaccctg ccgatcgacc agtatccgga tatcacgcct ccggtggtcc
     9301 aggtgaatgc ccagtttacc ggcgccgacg cacagaccgt ggagcaaacg gtggcaaccc
     9361 cgatcgaaga gcaggttaac ggcagtccgg gaatggaata catgcagagc aacagcacga
     9421 acaacggcct gttgtccatg aacgtcacct tcgggatcgg gacgaatgtc gatatcgcca
     9481 ctctggacgt gcagaaccgc gtgagcatag ccaatcccct cctgcccgca gtggccagcc
     9541 gcctgggcgt cacggtgcgc gccatcaacc ccagtcagct catgatgatc gccgtgtatg
     9601 cgccgcaagg gacgcatgac atcacgttcc tcgacaatta taccaatatc tttatccagg
     9661 acgcgctgct gcgtgttccg ggtgtgggga gcatctcgcg gtccaccgat gacttcagta
     9721 tgcgcgtttg gatggacccg caaaaaatgg cgtcgcataa cctgatgccg caggacatca
     9781 tcagtgcact gaattcgcag aacgtacagg tcgctgcggg ctctgccggc gtgccgccgc
     9841 aatccagggc acaggcgttc gagatcggca ttttggtgaa cggtcgtctg agtaaggtat
     9901 cggagtttga gaacattatt gtaaaaaaca acccggcacg gggggaattg gtctatctga
     9961 aagacgtggc gcgggtcgag ctgggcaaat tcacattcag cagtaactct ttcgtagacg
    10021 gacaccgggc ctccatcctg cgtatctacc aggcgccggg cagtaatgcc ctcgagacgg
    10081 cgggcggcat ttataaaaag ctggctttgc tgcgtcagag cttcccggcc gatgtcgaat
    10141 actccatacc cttcgaagcc gttaccgtcg tgaaggtctc gatggcggat gtcgtccgga
    10201 ccttattcct gaccctcggt ctcgtggtgc tggtggtatt cgtattcctg caaagtttcc
    10261 gctccacgct gattccgatc ctggctatcc cggtatcgat cgtcggcacg ttcattttct
    10321 tctatccgct gaactttacc atcaataccc ttaccatgtt cggcttcgtg ctggctatcg
    10381 gtatcgtcgt agacgacgcc atcatcgtgg tggaggcggt gcagcactat atggatgagg
    10441 aaggaatgtc acccaaggaa gcgacctacg tggcgatgaa ggatatctcc gcgccggtgg
    10501 tagccatcgc gctgatcctg gctgctgtct tcgtgccggt gggattcata ccgggtatcg
    10561 tcggccggct gtaccagcag tttgcgatca ccatcgccat ttcggtgatc ctttccgctt
    10621 tcatcgcact gtcattgacg cccgccttgt gcacgctgct gctgaaacct acggacccca
    10681 acaaaaagaa acgcgggctg agcaaaggat tcgaacactt taaccactgg ttcgacaggc
    10741 ttacgcagcg atacaaaaat ggagtggacc ggagcatcac tggtttccgg tggatcatcg
    10801 tactattggt cgtgatctgt atcggcgcct ggttcttttt cctgaaaaag cccaccggtt
    10861 ttattccttc cgaggacgac ggaaatctgt atgtcacctt tcagcttcca cccgcatcgt
    10921 ccaccgccca gagcgtggac gtgatgaaca ggctcatgga tgtcgtgtca aatacgcccg
    10981 gcgtggcgca ttatgccgcc ctgtcgggtc tcaatgtcgt caacaatgcg tccaactcca
    11041 atgccggaac gatctatatc cagctcaggc cctgggacga gcgggacaaa aagagcgagc
    11101 aggtgcccgg catcatcggg gtgctgaata aaaggatcgc ggatgcaggg atccggaatg
    11161 ccaatgccga ggtcattccc ccctccccta tccccggtgt cggtgaaaca gtcggtttca
    11221 gcatgcagat cgaacaacgg agtaccagcg atgatcttca ggccttccag cgcgtggtca
    11281 ataaatttgt tgccgaggtg aacaggaacc cggccattac cggcgcctat agtttttatt
    11341 ccgccaatac ccccggctac aaccttaagg tggaccgcga aaaatgcgag aagctgggcg
    11401 tcaatgtcgg cgatgtattc acgacgatcc aggcgttcat gggtagtctc tacatcaatg
    11461 attttaccga atacaaccgc actttccatg tcgtcgtgca ggcggatacc aattaccggg
    11521 cactgatcac ggatatgaac aaatactatg tccgcaatca ggacagtacg atggtgcccc
    11581 tgagcactct gtgtacgtat acgcctgcgg agacggcgcc gctgatccag catttcaata
    11641 ttttccggtc tgccgagatc gacggcggcc ccgcggcagg ctttagcagc aaccaggcga
    11701 ccgactcgct catcgcgatc gcgaacaggg tactgcccga agggtacaac tatgagttct
    11761 ccggtctgtc ctatgaggag atcaaggcag gttcgactac gatctatatc ttcctgttct
    11821 cgatcacctt cgtcttcctg ttcctggcgg ctttatacga aagttggtcg gtccccttct
    11881 ccgtcctgct ggctgtaccg gtgggcgcgt tcggagcgat ctttacgctt ttcctcgtgc
    11941 cgagcctcac cgataatgtc tatgcccaga tcggcctcat caccctgatc ggtctggcag
    12001 ccaagaatgc cattcttatc gtggaatttg ccaaagtgcg ggtagaccgc ggagaagagc
    12061 tgatccaatc cacgctggag gcggtgaaac tgaggctgcg tccgatcgtc atgacatcgc
    12121 tggcatttat tttgggtgtc ttgccgttgg tgctggccag cggagcgggc gcggtggcgc
    12181 gaaggacgat cgggtatacg gtgcttggcg ggatgaccgc cgcttccacc atcgcgatct
    12241 tcatcgtacc tgtactttac gtggtcatta cccgcatgtc ctacggcaag gaaaggctgg
    12301 cctggctgcg ggcgcatcat gaagaactta tggaaaagtc acggaaggtc gaagcgcaga
    12361 acatcgatcc ggaactggaa ttcgaattgg caagatcgcg taagcagaat agtcaacagg
    12421 gcgatgagaa taaaccacag cagggatgat tgcaaatacc tttatcaaac ggcccgttac
    12481 agcgatcgtg atctcgctgg tgctggtgat ctcaggtgtc atctccatct tcaacctggc
    12541 cgtggaccag tatcccgata tctcgccgcc cagcgtctcg gtcagaggca gctataccgg
    12601 ggcggacgcg gagacggtgg agcaaaccgt ggccattccc gtcgaagaac aggtcaacgg
    12661 tacgcccggc atggaataca tgcagagcac gagtaccaat agcggcggtg tcaatgtccg
    12721 cgtgaccttc cgcgtcggga ccaatgtaaa ggtagccgcg ctcaacgtcc agaaccgcga
    12781 ggggatcgca tcgccgatgc tgccctccgt ggtcagcaaa ttaggcatga cggtccgcgc
    12841 cagcaacccg gatcagctgc tgctggtggc gatctattcc cccaaaggtt cgcataatat
    12901 taccttcctc gacaactata ccagcgtgtt catccaggac gcgatcctgc gcgttccggg
    12961 tgtcggtgac gtgcaggccc ggacggatca gtttgcaatg cgtatctgga tggatccgca
    13021 aaaaatggct tcctaccatg tcacgccatc cgacgtgtcc gccgccgtca atgcacagaa
    13081 tcagtatgtc gccgccggtt cggccggagc gccgccgcag catatatcgc aatcctatga
    13141 gctgaacatt ctcgtgaacg gtatgctcaa taagcctgag gattatgaaa agatcgtcgt
    13201 caagaccaat cccaaaacgg gcgccatggt ctatctgaaa gacgtagccc gggtagagct
    13261 tggaaaattt acatttagca gcaatgcctt cgtggatgga aagcgctgtt cgctgctgat
    13321 gatctaccag gcgcccggca gcaatgctct cgagacggcc gacgcggtgt atgcaaagct
    13381 ggccgagctg aaaaagacct tccccaatga cgtcgactat aagatccctt tcgaggccat
    13441 cacgatcatt cgcgtctcga tggatgaagt ggaaaaaacg ttgctgcagg cgctggctct
    13501 ggtggcgctg gtcgtattgc tttttctgca aaactggagg tcgacgatca ttccgatctt
    13561 cgccattccc gtgtccattt tgggtacgtt ttgtttcttc atcccgctgg gattcaccat
    13621 caatacgctg accatgtttg gctttgtgct ggccatcggc atcgtcgtag acgatgctat
    13681 aattgttgtc gaggcggtgc agcattatat cgatgagcag catatgtccg cgaaagaggc
    13741 tacctatcag gccatgaagg agatctctgc gcccgtggtg gccatcgccc tgatcctggc
    13801 ggcggtattc gtgccggtgg gctttattcc gggcattgta ggccggctct accagcaatt
    13861 tgccatcacc atcgccatct cggtgatcct ctccgctttt atagcgctgt cgctgacacc
    13921 tgcgctgtgt accctactgc tgaagccgca caatccgcac aagaaaaaga actgggtaga
    13981 caaattcttc gcttcgttcg aaaaaggatt ctctgccttc acctatcgtt atgtccatgg
    14041 cgtccggcgg agcatcaaat attcgggaat taccgtactg gtgctggcgg ggatctgtgt
    14101 cggcgccttc tatcttttcc gtacgaagcc ctcgggcttc gtgccgtcgg aggacggcgg
    14161 ccgggtgtat atcacctttc aactggccga tgcgagcgca accacacaga gcgtggacgt
    14221 catgcaaaag atgatgaagg tcgtcaactc gacacccggt atcctgcatt atgtggccgt
    14281 gtccggcttc aacattctca acggtggcgc gacctcgaat gccggctcct tttttgtaat
    14341 gctgacaccg tgggatcagc gtaccacacc ccagacccaa tggcccggca tcagcaccga
    14401 actgtataaa cggttcgcca gggcaggcat aaagaatgca tcgatccacg tcatacagcc
    14461 ggcgccgatc cgtggtatcg gcatttcggc gggtttcacc ttcgagatag aacaaggcag
    14521 ctccacggat gatatttaca ccttcgagaa agcagtgaac cggtttgtgg cggaagccaa
    14581 aaagaacccc tctacgtcta cggccgtttg ctatttctcg gcgcatacgc ccagctatga
    14641 cctcacggtc gaccgcgaaa agtgccagaa gatgggggtg aacatttcac aggtattcca
    14701 gaccatgcag gccttcatgg gtagcctgtt catcggcaac ttcacactct acgatcgcac
    14761 ttttcacgtc gtgatgcagg ccgatacggc ctaccgtgca ttgatcgggg atatggacaa
    14821 atactatgtc cggaactcgc gcgattcgat ggtgccgctg tctactctca tcagctataa
    14881 accttcgcag gcggctccgt tgatcaccca tttcaatatc ttccggtcgg cggaggtcca
    14941 gggctcgatc cctcccggct atagcagcgg gcaaagcatc gatgacctga ggaaactggc
    15001 ggctaagtat ctgccacgcg gttacaccta tgatttctcg gggcttagct acgaggagat
    15061 caaggcaggc tcgatgacgg tgtatatctt cctcttttgt atcatcttcg tcttcctgtt
    15121 cctggcagct ttatatgaaa gctggtcgat accactgtcg gtgatgctgg ccgtccctat
    15181 cagcgcattc ggcgccattc ttgccttgtg gcttgctcct tcgctcaccg ataatgtata
    15241 tgcgcagatt ggactgatca cgctgatcgg tctgtccgcc aagaatgcga tcctgatcgt
    15301 ggaattcgcc aag
//
LOCUS       DASYSV010000515        14187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2538774, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000515 DASYSV010000000
VERSION     DASYSV010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 14187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14187
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538774"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..2113
                     /locus_tag="VGQ51_15985"
     CDS             155..2113
                     /locus_tag="VGQ51_15985"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEV9038136.1"
                     /translation="MSIMVNKITIAAALLLFSFVGEAQLTEKPPLHGNHWMAVTGKPL
                     AAEAGSQIFNKGGNAIDAACAMLGAVCTMWDVLSWGGETQALIYNPHTGKVIAIDALG
                     FAPTGATPAFFKAKGYNFPPEYGPLAAVTPGTPGGLCYMLAEYGTMSLKEVLAPAMEM
                     AKGYAIEAQTANSFERGKQRLKEWPYSKAIFLPHLGEKREAPEPGEVFVQKDLLETLT
                     KMVDAEQDALKHKKTRKEAIYAAMNRFYKGDIAQEFVRGCQEQGGLITMEDLAAWKPI
                     EEEPMHTNYKGVEVYKLQQWTQGPAMLQALNILENFDLKAMGYNSGRYINTIYQAMSL
                     AFADRDFYYGDPYFSTGQPMKGLLSKEYAKQRAALINPDHANPGAAPGDPYPFEGRTN
                     PFADMLKQRARTFGAPARGGNGGPSTGGAMNSTTMAAIDSLAFDHTVAGGPVDESLYH
                     DRLWRGTTSIEAADKDGWVISVTPSGGWPPACVAGHTGVGMSQRMQSFVLDSLIDPFN
                     VVEPHKRPRVTLTPTLALKDGKPYLCFSVQGGDTQDQNLLQFFLDMVDFGMTVQEATE
                     APNINSDQLWLSLGGERLEDRRPRPGRVLLGNVTPPYVRQELRQMGYFATYGDRTSGP
                     INAIYLDSKHGTFWGGSSNNGEDYGIGW"
     gene            complement(2095..4671)
                     /locus_tag="VGQ51_15990"
     CDS             complement(2095..4671)
                     /locus_tag="VGQ51_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038137.1"
                     /translation="MRNRTILFILCLFCLRTQAQDDQAVQTKADTWRQLVLQEKLFVH
                     TDKSFYLAGEVLWCRLYCVDGYTHRPQGVSKLAYVEILDADNRPVIQGKIRLDDGFGE
                     GSFALPPSVNSGTYRLRAYTNWMKNFGPGTFFEQTITIVNTIHGIDTAALTRPDTVKR
                     YPIGFFPEGGDLVAGLPSFVAFEMMDDEGRAIDAGGAILSGNNDTVTTFHARQAGLGV
                     FQLTPEPGQTYTAILNCPDGITRTWPLPPVLPKGYTMHLQDNGPDLTLTIHATPGLSA
                     HATPGLSSVAPSATPGLTALSAPVTPASQNIRLFLHSSTTSSFFDQKPVIGGDSAIFT
                     IPRSRLQDGNNRLTVFDAFGHPLAERQFFISPRQILHFSATTDQAVYSIRQKVRLDIA
                     LTDTTAAVPAPGLTHPDTPPAAVPAPGLTHPDTPPAAVQAAGLTHPDTTQAHLSIAVY
                     RLDSLQGYPATDILRYLWAGSEWTGPTPSATAFDDPQLAGLYSLTHGWSRFRWTDVLS
                     HPYGPRLYPPEIRGQIITGRLSETQTGAPAKNIIAYLSAPGTTFRFAAAGTDTGGRFQ
                     FDLKDYYGQDGIVINTGSPYKITTFSPFSEQYTDSRLPALHLTRDQLPLLGRYHVGIQ
                     VQNIFSGDSLRRFSLPNADTLPFFGRIGYTYLLDNYVRFTTMEEVLREYVREINVTRS
                     NGHLHLAMLDEPARSVFSDGNTLVLLDGVPVPDDRIFNYDPLKVRMLRVVPQEYILGA
                     AHFSGIASFTTYKGDFEGLALDSNSLQIDYEGLQYHRQFYSPAYPTARLAQSRLPDFR
                     NVLYWNPDYRVGRASAPVEFYTSDLPGEYLIVIQSLSSDGRAGVTYHRFTVQPAAYHP
                     IP"
     gene            complement(4674..5792)
                     /locus_tag="VGQ51_15995"
     CDS             complement(4674..5792)
                     /locus_tag="VGQ51_15995"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEV9038138.1"
                     /translation="MYRRLHIYISLMILAACQQTYAPPIIAKPPDYLVVEGFIENNGT
                     DSTIFTLSRTMRLDSFVFVPQVNCAMWVEGSDSTSYLLGDAGNGKYGALLTALNKNAT
                     YRIHIITIDGKEYASDYVPLVYNPPIDSISWVRQDNDVHKGVQVYANTHNPLDNTHFY
                     TWKYQECWEFHSPFESIYEYVPGVGVTPVTHIDDYVCWHYNDYSDILLASSTQLNHDL
                     IYDAPIVFIPDTSQKITVRYSILVTQYALTKDAFSWWKILQKNTEQIGSIFGVQPSAN
                     PGNIHCLTDTAELVLGYVDGGNSRSQRIFITNDQVLPWSYNSGCIEQSSKKYSIEELW
                     EMGELPIGVNFGDQSIIFSDKYCIDCRFTGTNVRPSFW"
     gene            complement(5792..8542)
                     /locus_tag="VGQ51_16000"
     CDS             complement(5792..8542)
                     /locus_tag="VGQ51_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9038139.1"
                     /translation="MRPVLPMMIVPLLLYGLTTRAQSRPAATITLDLEHVHIDSLARS
                     IEALLPVHFYYDTTQFDSLDVNLSVKNISLDQLFDRLFAGTPYHYATDVDGNVFLTKG
                     WIIRTGLSAPVAGSSDSIRTTVRYGEEKYARGKGSAENRLYNIGPPTNRLLPGKAVIS
                     GYVRNTRTGEPVAGVVITPDPPALATVTDKYGYYSLSLSRGRNVLHAQGLGLRDSRFQ
                     IMLYSDGKMDMDLREQVTTLREVIVSSQKTLNVNRVQLGVEQLSIEQIRQVPTVMGEA
                     DVLRVVLTLPGVKSVGEASTGLNVRGSSADQNLILFNDATIYNPSHFFGFFSAFNPEV
                     VKGIELYKSSIPAQYGGRVASVLDITPREGNKKEFTGTAGIGLLTSRVTIEGPLDKNK
                     TSFILGGRATYADWLLNLLPEQYKHSRASFNDVNLLISHNINEKNNLYFSGYISNDLF
                     NLNSDTVYRYGNRDVNVKWKHSFRKVTGTYTLGADRYRYLISSDGNPVNGFKLGFDIN
                     QLYLKADHTWYAASKHAVDFGFSTIRYILHSGDYEPYDSHSQVTPNRMQTEYGQESAL
                     YLSDRYTVTSSFSLVAGIRYSRFDYLGPHLQNDYPADQPRSILDTTGTTFYPNGKIIK
                     TYQGPEYRLSARYALTNSFSVKAGYNSLRQYTHMLSNTTSMAPTDIWKLSDTYIRPQL
                     GDQVSLGFYKNLFNNTIETSVEVYYKNLHDYLDYRSGATLILNPHIETDVLNSKGKAY
                     GVELMVRKTAGKLNGWISYTWSRTFLRTDDPTAGETVNKGRWYPADFDIPTDLSFVGN
                     YKINHRFSISTNVIYYTGRPITLPITAYYYANSERVYYSDRNAYRIPDYFRADFSMNI
                     EGNFKVHQKTHNSWTIGIYNFTGQKNAYNVYFESVNHVVNGVKLSIFGSAIPFVNFNI
                     HF"
     gene            complement(8545..9264)
                     /locus_tag="VGQ51_16005"
     CDS             complement(8545..9264)
                     /locus_tag="VGQ51_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038140.1"
                     /translation="MPTIQTNCALQALFFFTLTLSSRAQPRQPDSISSPAAARAALYY
                     QSYIGANAAIYNGCVYQQTYIGVEGSPYFLSDNLTDGSVVYEDLPYTHLLLLYNTVLD
                     QPVLADRQGRLLSLPNGKVSQFTIDGHIFIHLSAGNIPSGYYELLRSGYATLLLRHTK
                     RLEEKLGGEIRRYITSQENYYVYKNGHYYSFGSVNGLISLLSDKQQQLQRYRRSEHIR
                     FRKDPLTAAERLIDYYNQLPR"
     gene            complement(9338..9979)
                     /gene="msrA"
                     /locus_tag="VGQ51_16010"
     CDS             complement(9338..9979)
                     /gene="msrA"
                     /locus_tag="VGQ51_16010"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEV9038141.1"
                     /translation="MKPLVFVLAFSVITACGQTQPVNIPVKTSDNPGPNEAVADFAEG
                     CFWHTEIVFQSLVGVRDAVSGYCGGTDTHPDYEKVCTGTTGHAESVQVYYDPSKISYA
                     TLVKAFFASMDPTELDRQGPDEGTQYRSIAFYRNPQEKKIIEDEIARLTAQKKYSRKI
                     VTEVQPFKAFYPAETYHQEYIKNHPENPYVQHVSIPDWLQFKQANKGQFEFKS"
     gene            complement(9981..10520)
                     /gene="msrB"
                     /locus_tag="VGQ51_16015"
     CDS             complement(9981..10520)
                     /gene="msrB"
                     /locus_tag="VGQ51_16015"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV9038142.1"
                     /translation="MKYSILLLAAFISLAAAAPAAQAQSNKYPDPPEASAKSKYPMTL
                     TDVEWKSRLTPDQYFILREKGTERAFTGKYDHFYQKGTYYSAATLQPLFSSDTKFNSG
                     TGWPSFYAPINPDAVRLIKDFSDGMERWEVVDSKSGSHLGHVFDDGPAPTGKRYCMNS
                     AALIFVPEGGKPPTSSKTN"
     gene            10622..12985
                     /locus_tag="VGQ51_16020"
     CDS             10622..12985
                     /locus_tag="VGQ51_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038143.1"
                     /translation="MFKSYLKVAFRNLWKNKGFSFINIAGLAIGMASAILILLWMQNE
                     ISYDDFHEKKDRIYEVWNRATFSGALHCWNTTPKVLAAAMQKDLPEVEHAIRADWVGS
                     HLFSVGEKRLMIRGNAVDSSFLQVFTFPMSEGDPKSALVDPYSIVVTRSLAKTLFGAT
                     DAIGKVVRIDNKDNYHVTGVIEDLPNNTRFDFQYLVPWNFVRRQGNDDSWWGNNSTRT
                     YVLLKPNATMASINSKLLGYKERYDKEEKHWEMFVYPMDRWRLHSRFEGSVETGGRIT
                     FVRLFGIVAGFILLIACINFMNLSTARSEKRAKEVGIRKVVGAPRGRLIGQFIGESVL
                     LSFLAALLAIGIVLISLPGFNMLTQKHLYIPFGSVWFWLSAVAFILFTGLLAGSYPAF
                     FLSSFQPVKVLKGTFRAANALVTPRKVLVVLQFTFAITLIICTIIVKEQIDHARERES
                     GYDQGNLIYHFLTGDLEKNYELVRNDLLREGVATAVCKTSSPITNNYSDTWGFVWQGK
                     DPEDKTDFLMTCADRGMTHTTGLQLAEGRDFDPENYPTDSAGIVLNESAVRAMHFKQP
                     IGQIITEDTMHFHVIGVVKDFIIESPYSPIRPMVIRGARGGWFSVMNIRLNDKNLTET
                     NLKKAAAIFKRYNPEYPFEYHFVDEDYSRKFDDEKRTGTLAGLFAALTVFISCLGLFG
                     LATYMAENRIKEIGVRKVLGASVTGIATLLSKDFLVLVFISFFVAGPIAWMAMHAWLK
                     GYAYRVDIHWWVFAMAAALSAGIALVTVSFQAVRAGLTNPARSLRSE"
     gene            13071..>14187
                     /locus_tag="VGQ51_16025"
     CDS             13071..>14187
                     /locus_tag="VGQ51_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038144.1"
                     /translation="MRKCTLSLTLALAFFALPLLLSAQTDSSRYAIGAVSLNKDLTQA
                     ITIRGEDLEKMPFMNLSDALGAWLLGVYAQPGALVYVVDGNPVTDVNLYPIYDIAEVT
                     WLPNAVATAPYGNGLQELVLITTKRGKGNNGLRFSAQTGLIKGGQFDGPTHTGFYHQY
                     YLGVRRDTGKLSYGFSADWQQDEIASSQTASLSQYTPPTLKRWRLNGRVEWRPNAQNA
                     VRLTLGYAPQDVGYIVNSGGGGYQFHYSDWWYNHLLVPALSWEWRSHGLHNKFDAEYL
                     YSWSRLRYDETDTVDNSGTTVLESGYTQGTGKNSHLVLRDRLSYEAKAGNWKVVPAVD
                     ISYQHIDERSDYKEFNPYNPTATNLTSNVEFKGSLFYI"
BASE COUNT         3129 a         3219 c         4573 g         3266 t
ORIGIN      
        1 ggtggagaat atgacacgat ggatccgaag tatatggaat ggatgagtag acaggtgaag
       61 gatggccggt atttgtattg tcccgagggc agtcattgtg acatgtggga tgaccagcaa
      121 cattattttc ccggactgat aaaattctta caagatgtcg ataatggtaa ataagatcac
      181 gatcgcagcg gcgctgttgc ttttttcgtt tgtgggtgag gcccagctga ccgagaagcc
      241 accgttgcat ggcaatcact ggatggcggt gacgggcaag ccgctggcgg cggaagccgg
      301 ctcgcagata ttcaacaagg gcggcaatgc catcgatgcg gcctgtgcta tgttgggggc
      361 ggtgtgtacc atgtgggacg tattgagctg gggcggcgag acgcaggcgc tgatctataa
      421 tccgcatacg ggtaaagtta tcgccatcga tgcgctgggg tttgcgccga cgggggccac
      481 tcctgctttt ttcaaggcga agggatacaa ttttccgccg gaatacggac cgttggcggc
      541 ggtgacgccg gggactccgg gaggactttg ttatatgctg gcggaatatg ggacgatgag
      601 cctcaaagaa gtattggcgc cagccatgga aatggcaaag ggatatgcga tcgaagcgca
      661 gaccgccaac agttttgaac ggggaaaaca acggttaaag gaatggccgt acagcaaggc
      721 catctttctg ccgcatctgg gagagaagcg cgaggcgccg gagcccggtg aggtctttgt
      781 tcagaaggac ctcctggaga cactgaccaa aatggtggat gcggagcagg atgcgttgaa
      841 gcataagaag actcgtaagg aagccattta tgccgcgatg aaccggttct ataagggcga
      901 tatcgcgcag gaattcgtca ggggttgcca ggagcagggt ggattgatca ccatggagga
      961 cctggcggca tggaagccta tcgaggagga gccgatgcat acgaattata agggtgtcga
     1021 agtgtataag cttcagcagt ggacgcaggg gccggccatg ctgcaggcgt tgaatatcct
     1081 ggagaatttc gatctgaaag cgatgggcta taacagcggc aggtatatca atacgatata
     1141 tcaggcaatg agcctggcgt tcgccgacag ggatttctat tatggcgacc cgtatttttc
     1201 gacgggacaa ccgatgaagg gtttgctgag caaagaatat gcgaaacagc gggcggcact
     1261 gatcaatccg gaccatgcga atccgggggc tgcgccgggc gatccatatc ctttcgaggg
     1321 aaggacgaat ccgtttgcgg atatgttgaa gcagcgggcg aggacttttg gggcgcctgc
     1381 aaggggtggg aacggagggc cgagtacggg tggagcgatg aattcgacga cgatggccgc
     1441 gatcgatagc ctcgccttcg atcatacggt agccggcggg cctgtggatg aatccttata
     1501 tcatgaccgt ttgtggcgcg gaacgacgag catcgaggcc gccgataagg acggatgggt
     1561 gatctcggta acgccaagcg ggggttggcc gccggcatgt gtagcgggac atacgggcgt
     1621 aggcatgagc cagcggatgc agagttttgt gctcgacagc ctgatcgatc ccttcaatgt
     1681 ggtggagccg cacaaacggc cgcgcgtaac gctcacgccc acactggcat tgaaggatgg
     1741 gaagccgtat ttgtgtttca gcgtacaggg cggcgatacg caggatcaga acctgctgca
     1801 attcttcctg gacatggtcg atttcggaat gacggtacag gaggcgaccg aagcgcccaa
     1861 tatcaactcg gaccaattgt ggttgtcgct gggtggcgag cggctggaag accgtcgtcc
     1921 aagaccggga cgggtattat tgggaaatgt gacaccgcca tatgtccggc aggagctgag
     1981 gcagatggga tattttgcga catatgggga ccggacgagc ggaccgatca atgccattta
     2041 tctcgactcg aagcatggaa ctttctgggg agggagcagc aataacggcg aggattacgg
     2101 tatcgggtgg taggcggccg gttgcacggt gaaccggtga taggtaacgc ctgccctgcc
     2161 atcggaagac aggctctgga tcacgatgag atattcaccc ggcagatcgg atgtgtagaa
     2221 ttctaccggc gccgatgcgc gtccgacgcg ataatcggga ttccaataga ggacatttct
     2281 gaaatccggc agacggcttt gcgccaggcg ggccgtcgga taggcaggcg aatagaattg
     2341 ccggtgatat tgcagaccct cataatcgat ctgcagcgaa ttgctgtcca gtgcgagacc
     2401 ttcgaagtct cccttatagg tggtgaagct ggcgatgccg ctgaaatggg cggcgccgag
     2461 aatatattcc tgcggaacga cccggagcat cctcactttc agcggatcgt aattgaatat
     2521 cctgtcatcc ggaacgggga ccccgtccaa cagcaccagc gtgttcccgt cactgaacac
     2581 cgaacgggcc ggctcatcca gcatcgcgag atgcagatgg ccattgctgc gggtcacatt
     2641 gatctcgcgg acgtattcgc gaagtacttc ctccatcgtg gtaaaccgaa cataattatc
     2701 cagcaggtag gtataaccga tgcggccgaa gaacggcaag gtgtctgcgt tggggagcga
     2761 gaaccttctc aggctgtctc cggagaagat attctgtacc tggatgccga catggtaccg
     2821 gcccagcaat ggcagctggt cgcgggtgag gtggagcgcc ggtaggcggc tatcggtata
     2881 ctgttccgaa aaaggactga acgtggttat tttataggga ctgccggtat tgatcacgat
     2941 gccatcctgt ccatagtagt ctttcaggtc aaactggaat cttccgccag tgtctgttcc
     3001 ggcggcagcg aaccggaagg tcgtgccggg cgctgacagg taggcaatga tattttttgc
     3061 aggggcgccg gtttgcgtct cggagagacg gccggtgatg atctgtccgc gaatctcggg
     3121 tgggtagaga cggggaccat aggggtgcga aaggacatcg gtccaacgga atcttgacca
     3181 gccgtgtgtc agggaataca gacctgcgag ttgagggtcg tcgaatgcgg tagccgatgg
     3241 cgtcgggcct gtccattcgg aacctgccca gagatatcgg aggatgtccg tcgccggata
     3301 gccttgcagg gagtcgagcc ggtagacggc gattgagagg tgggcttgcg tagtgtcggg
     3361 gtgtgtgagg ccggcagcct ggacggcggc aggtggggtg tcgggatgtg tgaggccggg
     3421 agccgggact gcggcaggtg gggtgtcggg atgtgtgagg ccgggagccg ggaccgcggc
     3481 cgtagtgtcg gtcagcgcga tgtcgagccg caccttttgc cggatggaat agaccgcctg
     3541 atcggtggtc gcggagaaat gtaatatttg tcggggtgag atgaagaatt gtcgttcggc
     3601 cagcgggtgt ccgaacgcgt cgaaaacggt cagacgattg ttgccgtcct gcagccggct
     3661 gcgcgggatg gtaaatatcg ccgaatcccc gccgatgacg ggtttctgat cgaagaagga
     3721 ggaggtcgtg gaagagtgaa gaaagagccg gatattttgg ctggcggggg tgacgggggc
     3781 ggagagggcc gtgagaccgg gggtggcgga tggggcgacg gaggagaggc cgggggtggc
     3841 gtgggcggag aggccggggg tggcgtggat ggtaagggtg agatcggggc cgttatcctg
     3901 aagatgcatc gtatagcctt tgggaaggac gggcgggagc ggccaggtgc gggtgatacc
     3961 gtcggggcag ttaagaatgg cggtgtaggt ttgaccgggc tcgggggtga gctggaagac
     4021 gccaaggccg gcttgacggg catggaaagt ggttaccgta tcgttgttgc cgctgaggat
     4081 cgcaccgccc gcatctatcg cccggccctc gtcatccatc atttcaaacg ccacaaagga
     4141 agggagtccc gcgaccaggt cgccgccttc gggaaaaaat ccgatcgggt agcgtttgac
     4201 ggtatcggga cgggttagag ctgcggtgtc tattccgtgt atcgtattga cgatcgtgat
     4261 ggtctgttca aaaaaggtcc cgggtccaaa attcttcatc cagttggtgt aggcgcgcag
     4321 gcggtaggtc ccggaattca ccgatggcgg cagggcaaaa gagccttcgc cgaaaccgtc
     4381 atccagtctg atctttccct gaatgaccgg ccggttatcg gcatcgagga tctcgacata
     4441 ggcgagcttg ctgacgccct gcggccggtg ggtatagccg tcgacgcaat acaggcggca
     4501 ccaaaggacc tcgccggcga gatagaagga tttgtccgta tgcacgaaca gtttttcctg
     4561 caggaccagt tggcgccagg tgtcggcctt tgtctgtacg gcctgatcgt cctgggcttg
     4621 tgtccggagg caaaagaggc aaagtatgaa gaggatggtt ctgtttctca tgtttaccag
     4681 aaggatgggc ggacgttagt gccggtgaat cgacagtcta tgcaatactt atcggagaag
     4741 atgatcgatt gatccccaaa atttacgccg atgggcagct cacccatttc ccagagctcc
     4801 tcaatgctat attttttgct cgactgctcg atacagccac tgttgtagga ccatggcagt
     4861 acctggtcgt tggtgataaa gatgcgttgt gacctgctgt tgccgccatc gacgtatccg
     4921 agcaccagtt cggctgtatc ggtaagacaa tgaatattgc cggggttggc ggaaggttgc
     4981 acgccaaaga tggagccgat ctgttcggtg ttcttttgca ggatcttcca ccagctgaaa
     5041 gcgtctttcg taagggcata ttgcgtcacg aggatgctat agcgtactgt tatcttctga
     5101 gacgtgtcgg ggatgaagac gatcggcgcg tcatagatga gatcgtggtt cagctgggtg
     5161 gagctggcca ggaggatgtc agagtaatcg ttatagtgcc aacagacata atcgtcgata
     5221 tgggttaccg gcgtgacgcc gaccccgggg acatattcat agatggactc gaacggggag
     5281 tggaattccc agcattcctg gtatttccag gtgtaaaaat gtgtattgtc gagaggattg
     5341 tgggtattgg cgtagacctg aacgccctta tgaacgtcgt tgtcctgtct tacccagctg
     5401 atgctgtcga tgggtggatt ataaaccagc ggaacataat cggacgcata ttcttttcca
     5461 tcgatggtga tgatatggat gcggtaggtg gcgttcttgt tgagcgcagt caggagcgcg
     5521 ccgtatttac cgttgccggc gtcaccgagc aggtatgagg tgctgtccga tccttctacc
     5581 cacatcgcac agttgacctg gggtacgaag acaaagctgt cgagtctcat ggtacgcgag
     5641 agtgtaaaga tcgtggagtc tgtgccgttg ttttcgatga acccttccac gacgagataa
     5701 tccggcggtt tggcgatgat cggcggcgca taggtctgct ggcaggccgc caggatcatt
     5761 aaggatatgt atatgtgaag ccgtctgtac atcagaaatg gatattaaag ttaacgaatg
     5821 ggatggcgct gccgaagatg gacagcttca ctccgttgac gacgtggtta acggattcga
     5881 aatagacatt gtaggcgttc ttttggccgg tgaaattgta gatgccaatg gtccacgaat
     5941 tatgggtctt ctggtggacc ttaaaatttc cttcgatgtt catcgagaag tcggcccgga
     6001 aatagtcggg gatgcgatag gcattccggt cggaatagta caccctttcc gaattcgcgt
     6061 aatagtaggc ggtaatgggt aaggtgatgg ggcggccggt ataatagatg acattggtcg
     6121 agatgctgaa ccggtgattg atcttataat tgcccacgaa tgacagatcg gtggggatat
     6181 cgaaatcggc aggataccag cgacccttgt tgaccgtctc gcccgcagtg gggtcatcgg
     6241 tcctgagaaa agtgcgcgac caggtatagc tgatccatcc gttgagcttg ccggccgtct
     6301 tgcgtaccat cagctcgacc ccatacgcct tcccttttga attgagcacg tcggtctcga
     6361 tgtgggggtt caggatgagc gttgcacccg agcgataatc gaggtagtcg tgcaggttct
     6421 tataatagac ctctacggag gtttcgatgg tattgttgaa gagattcttg tagaaaccca
     6481 gcgagacctg gtcgcccagc tggggccgga tataagtgtc gctcaacttc cagatatcgg
     6541 tgggggccat ggaagtggta ttggagagca tgtgtgtata ttggcggaga gaattgtagc
     6601 ctgcctttac agagaaggaa ttggtgaggg cataacgcgc cgagagacgg tattcgggcc
     6661 cctgataggt cttgatgatc tttccgttcg gatagaacgt ggtgccggtg gtatcgagta
     6721 tcgacctggg ttgatccgcg ggatagtcgt tttgcaaatg cgggccgaga taatcgaacc
     6781 tggaatagcg gatgccggcg acgagagaaa aggacgatgt caccgtatag cgatcggata
     6841 gataaagcgc gctttcctgt ccgtattcgg tttgcatcct gttgggcgtt acttgtgagt
     6901 gggaatcgta cggctcatag tcgccggaat gaaggatata ccggatggtg ctgaacccga
     6961 agtcgacggc gtgtttcgag gcggcgtacc aggtatggtc ggcttttaag tagagctggt
     7021 tgatatcgaa gcccagcttg aaaccattca ccgggttgcc gtcgctggaa atgagatacc
     7081 ggtaacggtc cgccccgagg gtataggtgc cggtgacttt gcggaagctg tgtttccatt
     7141 tgacattgac atcgcggttg ccgtaccggt agaccgtgtc gctgttcaga ttgaaaagat
     7201 cgttgctgat atagccgctg aaatagaggt tattcttttc gttgatgtta tggctgatga
     7261 ggagattgac atcgttgaaa gaggcgcggc tatgtttgta ttgttcgggg agaaggttga
     7321 gcagccagtc cgcgtaggtg gcgcggccac ccaggataaa ggaggtcttg ttcttgtcga
     7381 gaggtccttc gatggtgacc ctgctggtga gcagaccgat gccggcggtg ccggtaaatt
     7441 cttttttgtt gccttcgcgc ggggtgatat cgaggacgga agcgaccctg ccaccgtatt
     7501 gggcggggat gctgcttttg tacaattcga tcccttttac gacctccggg ttaaaggcgg
     7561 agaagaatcc gaagaagtga gacgggttat agatggtcgc gtcgttaaag aggatgagat
     7621 tctgatcggc cgagctgccg cggacgttga gaccggtgct ggcttctccg acgctcttga
     7681 cgccgggaag ggtgagcacc acacggagca catcggcttc tcccataacg gtcggcacct
     7741 gtcggatctg ctcgatgctc agctgttcga cccccagttg gaccctgttg acatttagcg
     7801 ttttttgcga agagacgatc acctcgcgga gggtggtgac ctgttcgcgg aggtccatgt
     7861 ccattttccc atcggaatag agcatgatct ggaaacggct gtcgcgaaga ccgagtccct
     7921 gcgcatggag gacattgcgg cccctggaca gggatagtga ataatagccg tatttatctg
     7981 tgaccgtggc gagggccggg gggtcgggag tgatgaccac gccggcgacg ggttcccctg
     8041 tgcgggtatt gcggacatat cccgagatga ccgccttgcc tggcaggagc cggttggtcg
     8101 gtggcccgat attatagagt ctgttctccg cgctgccctt gccccgtgca tatttttctt
     8161 caccataacg gacggtcgtg cggatgctat cggacgagcc ggctacagga gcggatagcc
     8221 cggtccggat gatccagcct ttcgtgagga agacattgcc gtccacgtct gtcgcatagt
     8281 ggtaaggggt accggcgaac aatcgatcga acaactggtc gagacttata ttctttacgc
     8341 tgagattgac atccaggctg tcgaattggg tggtatcgta atagaaatga acaggcagga
     8401 gggcctcgat gctgcgggcg agactatcta tgtgaacatg ttccagatcg agggtgatgg
     8461 tcgcagccgg acgggactgt gcccgggttg taaggccgta cagcagcagc ggaacgatca
     8521 tcatcgggag gacaggacgc atattcagcg gggtaattga ttatagtaat cgatcaggcg
     8581 ttcggctgcc gtcagcgggt ccttgcggaa ccggatatgt tccgagcgtc ggtaacgctg
     8641 gagttgttgt tgcttatcgg agagcaggct gatgagaccg ttgaccgatc cgaaggagta
     8701 gtaatggcca ttcttataaa cgtagtaatt ttcctgcgac gtaatatagc gccggatctc
     8761 gccgccaagt ttttcttcca gtcttttcgt atggcggagc aggagggtgg catagccgga
     8821 gcgaaggagc tcataatacc cggagggaat attacctgcg gagagatgaa taaagatgtg
     8881 tccatctatt gtgaattggc tgaccttccc gttcggcagg gagagcagcc ggccctggcg
     8941 gtcggcgagg acaggttggt cgaggacggt attatacaga aggagcagat gggtgtaggg
     9001 gagatcttca taaaccacgg agccatcggt aaggttgtcg ctcaggaaat agggggaacc
     9061 ttcgactccg atataggtct gctgatagac gcagccgtta taaatggcgg cattggcgcc
     9121 gatgtaggac tgatagtaga gagccgctct tgccgcggcg ggagaagata tgctgtcggg
     9181 ttgccggggc tgcgcccggg aggatagggt caatgtgaaa aagaataacg cctgcagggc
     9241 acagttagtt tgaatggtcg gcatatgcaa tctgaggttg taggcctaag ataacgaaaa
     9301 aagggtccgc ctgaggatta aaatcggtga ctgctgttca ggatttgaac tcgaactgac
     9361 ccttattggc ctgtttgaat tgcagccaat cgggaatgga tacgtgctgt acgtaaggat
     9421 tctccggatg gtttttgata tattcctggt gataggtttc ggccgggtag aaggccttaa
     9481 agggttgcac ctcggtgacg atcttgcggg aatatttttt ttgtgccgtc aggcgggcga
     9541 tctcgtcttc gatgatcttc ttctcctgcg ggttgcggta aaacgcgatc gagcggtatt
     9601 gtgtgccttc gtcggggccc tggcggtcga gctcagtagg gtccatcgag gcgaagaagg
     9661 cttttacgag ggttgcgtag gatatcttcg aaggatcata gtaaacctgg accgactccg
     9721 cgtgaccggt cgtgcccgtg cagacctttt cgtagtccgg gtgtgtatcg gttcctccgc
     9781 agtagccgga gacggcatcg cgaacaccta ccaggctttg gaagacgatc tccgtatgcc
     9841 agaaacatcc ctcggcaaaa tcggcaacgg cctcattggg acccgggtta tcggatgttt
     9901 tgacggggat attcacgggt tgtgtctgac cgcaggcggt tatgacggaa aaggcgagga
     9961 cgaagacgag gggcttcata ttaatttgtt tttgagctgg ttgggggttt gccgccttcg
    10021 ggaacgaaga tgagggcggc ggaattcatg caatagcgtt tgcccgtggg ggcgggtccg
    10081 tcgtcgaata catgaccgag gtgcgagccg cttttgctat ccaccacttc ccatctttcc
    10141 atcccatcgc tgaaatcctt gatcagacgt actgcatccg ggttgatcgg ggcgtagaaa
    10201 ctgggccatc cggtaccgga attgaatttg gtatccgaac tgaataacgg ttgcagggtc
    10261 gcggcggaat aataggtccc tttttgatag aaatggtcgt atttaccggt gaaggccctt
    10321 tcggtgcctt tttcccggag gataaagtat tgatcgggcg tcaggcggga tttccattcg
    10381 acatccgtga gggtcatggg gtatttggac tttgcagatg cttcgggggg atcggggtat
    10441 ttattcgact gagcctgtgc ggccggggcg gcggcggcca gcgagataaa ggcggcgagc
    10501 aacaggatgg aatatttcat gcgttgggta ttatacccta aaataacgaa agcggaggag
    10561 aaagggtttg ttaaagtgca acatttcgcc ggttggcgcg tcctatttga aaccgttttt
    10621 catgttcaag agttatctga aagtcgcttt taggaatctt tggaagaata aggggttctc
    10681 gttcatcaat atcgccgggc ttgcgatcgg gatggccagc gccatcctga tcctgttgtg
    10741 gatgcagaat gagatcagct atgacgattt tcatgaaaaa aaggaccgga tctatgaagt
    10801 ttggaaccgg gcgaccttca gcggagcgtt gcattgctgg aatacgaccc caaaagtgct
    10861 ggcggcggcc atgcagaagg acctgcccga agtggagcat gccatacgcg cggactgggt
    10921 ggggtctcac ttgttcagtg tgggggaaaa acggctgatg atccggggta acgccgtgga
    10981 ctccagcttt ttacaagtgt tcactttccc tatgtcggag ggggatccga aatcggcgtt
    11041 ggtagacccg tacagcatcg tggtcacgag atcgttggcc aaaactcttt ttggcgcgac
    11101 cgacgccata gggaaggtcg tccggataga caataaggat aattatcatg tgacgggagt
    11161 catcgaagat ctgcctaaca atacccggtt tgatttccag tacctggtgc cctggaactt
    11221 tgtccggcgg cagggaaatg acgattcgtg gtgggggaat aactcgaccc ggacctatgt
    11281 cctgctgaaa cccaatgcga ccatggcttc tatcaactca aagttgcttg ggtacaagga
    11341 gcgttatgac aaggaggaga aacactggga gatgttcgtg tatccgatgg accggtggag
    11401 actgcattcg aggttcgaag ggtcggtaga aacgggggga cggatcacct tcgtaaggtt
    11461 gttcggcatc gtggccgggt ttattctcct gatcgcatgc atcaatttta tgaacctgag
    11521 tactgcaagg agcgagaaga gggcaaagga agtgggcatc cgcaaagtcg tgggggcgcc
    11581 ccgggggagg ctgatcgggc aatttatcgg agagtcggta ttgttgtcct ttttggccgc
    11641 gctgctggcg atcggtatcg tgctgatctc cttacccggg ttcaatatgc tgacgcaaaa
    11701 gcatttgtat ataccttttg ggagtgtatg gttctggctg agcgccgtgg cgttcatcct
    11761 gttcaccggt ctgctggcgg ggagctatcc ggcttttttc ctttcttctt ttcagccggt
    11821 gaaagtgctg aaggggacgt tcagggccgc caatgcgctg gtgacacccc ggaaagtgct
    11881 ggtggtgctg cagttcacct tcgccattac gctgatcatc tgtacgatca tcgtcaaaga
    11941 gcagatagac catgcgcggg agagggagag cgggtatgac cagggtaacc tgatctatca
    12001 ttttctgacc ggcgatctcg agaagaatta cgagctggtg cggaatgatc tgctgaggga
    12061 gggcgtggct acggcggttt gtaaaacgag ctcgcctatt acgaacaatt acagcgatac
    12121 gtggggtttc gtatggcagg ggaaggaccc tgaggacaag accgatttcc tgatgacctg
    12181 tgccgaccgg ggcatgacgc atacgaccgg gttacagctg gcggaaggca gggacttcga
    12241 tccggagaat tatcctacgg attcggcggg tatcgtgctg aatgagtcgg cggttcgggc
    12301 catgcatttc aaacagccga tagggcagat catcaccgag gatacgatgc attttcatgt
    12361 tatcggggtg gtaaaggatt tcattatcga atcgccctat tcgccgataa gaccgatggt
    12421 gatccgggga gcgcgtggtg gttggttcag cgtgatgaat atccgcctca atgacaagaa
    12481 cctgactgag accaatctga aaaaggcggc ggcaattttc aaacgctaca accccgaata
    12541 tcccttcgaa taccattttg tggatgaaga ctattcgagg aaattcgacg atgagaaaag
    12601 gaccgggacg ctggcgggat tgtttgccgc attgaccgtg ttcatatcct gtctcgggtt
    12661 gttcgggctg gcgacgtata tggcggagaa caggatcaag gagataggcg tccgcaaggt
    12721 cctgggcgcc tcggtgacgg ggatcgcgac cctgttgtcg aaggatttcc tggtgcttgt
    12781 atttatatcc ttctttgtcg cgggcccgat cgcctggatg gcaatgcatg catggctgaa
    12841 agggtatgca taccgggtgg atattcattg gtgggttttt gcgatggcgg cggcgttatc
    12901 ggcgggtatc gcgctggtga ccgtcagttt ccaggcggtg agggcgggat tgacgaatcc
    12961 ggcgaggagc ctgcgctcgg aataggcgga ttataatttc gtgaaaatgg agacaggttg
    13021 gctatagcgt cgcggatagc tattatcttt aatagcgaag aacaccactt atgagaaaat
    13081 gtaccttatc gctgaccctg gcgctggcct tttttgccct tcccttgctg ctgtccgccc
    13141 agacggacag ttcgcgatat gcgatcggcg ccgtttccct gaataaagat cttacccagg
    13201 caatcacgat ccggggcgag gacctggaga agatgccttt tatgaacctg agcgacgcgc
    13261 tcggcgcatg gttattgggc gtttatgccc aaccgggagc cctggtctat gtggttgacg
    13321 gtaatcccgt gacggatgtc aatctatatc ccatctatga cattgccgaa gtcacctggc
    13381 ttccaaacgc agtggcgacg gccccctatg gcaatgggct acaggagctg gtgctgatca
    13441 caaccaaacg tgggaaaggg aacaacggcc tgcgtttctc ggcgcagacg ggattgatca
    13501 aaggcggcca gttcgatggg cccacccata ccggttttta tcatcaatat tatctcggcg
    13561 tccggcggga cacgggcaag ctgagctatg gcttttcggc agactggcaa caggacgaaa
    13621 ttgcgtcgtc gcagacggct tctttatccc agtatacgcc gcccaccctc aaacgctggc
    13681 ggttgaacgg ccgtgtggaa tggaggccga atgcgcagaa tgcggtccgg ctcaccctgg
    13741 gctatgctcc acaggatgtg ggatacatcg tgaattcagg gggaggcggg taccaattcc
    13801 actattcgga ctggtggtac aatcatttgc tggtaccggc cctgagctgg gaatggcggt
    13861 ctcacggtct gcacaataag ttcgatgcgg aatatttata cagctggtca cgtctgcgtt
    13921 atgatgagac ggataccgtc gacaatagcg ggacgaccgt ccttgaaagc ggctatacgc
    13981 agggtacggg gaagaactcc catctcgtgc tcagggaccg gctgagctat gaagcgaagg
    14041 cgggcaattg gaaagtggtt ccggccgtcg atatcagcta tcagcatatc gatgagcggt
    14101 cggactacaa agaattcaat ccatataacc ccacggcaac gaatcttacc agtaacgtgg
    14161 aattcaaagg cagtcttttt tatatca
//
LOCUS       DASYSV010000516         7882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2413101, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000516 DASYSV010000000
VERSION     DASYSV010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413101"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VGQ51_16030"
     CDS             complement(<1..648)
                     /locus_tag="VGQ51_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV9038145.1"
                     /translation="MDDNNTPTETQDNNRIVPVNIEEQMKTAYIDYSMSVIVSRALPD
                     VRDGLKPVHRRILFGMNELGNHSNKPYKKAARIVGDVMGKYHPHGDSSIFDAMVRMAQ
                     EWSMRYMLVDGQGNFGNQDGDGPAAMRYIEARLHRQAEYMLEDIEKDTVDFQPNFDDS
                     LQEPTVLPTRLPQLLMNGSSGIAVGMATNMMPHNLSECIDGCVAYIDKRDISIEEL"
     gene            complement(854..1351)
                     /locus_tag="VGQ51_16035"
     CDS             complement(854..1351)
                     /locus_tag="VGQ51_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEV9038146.1"
                     /translation="MKNYILTQAVADKKLRRMAYELLENNSGEEQLILAGIRESGSVI
                     ARNIQRLLQEIAGDRLRTRLITITLDKKEPGPVTLSESLSFDGQVIVVIDDVANSGKT
                     LLYAMKPFLEAHPRKIECLVLVERSHKAFPIQPDYTGLSLSTTLQEHIYVEVDGDQVR
                     GAWLE"
     gene            1414..2736
                     /locus_tag="VGQ51_16040"
     CDS             1414..2736
                     /locus_tag="VGQ51_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV9038147.1"
                     /translation="MALSQQIVFLLVLATAIILFTKKIRWVRRNILLGKDEDYSGQSG
                     RRWKNVLLLAFGQKKMFRNPMVAVMHFVVYAGFIIINLEVLEIVLDGILGTHRLFYFA
                     GPLYLGLIDAFEILAAGVFLACFIFFCRRNVVKLRRFISHDLDGWPRSDANYILLTEM
                     ALMALFLTMNAADTLLQSRGYDHYAAHITGDFWFSQWLHPLLSGWSNGALVVLERTCW
                     WLHILGILAFLNYLPYSKHLHILLAFPNAYYARLQPEGRIRNMPSVQNEVLYAMQPET
                     APPAPADGSVPPAPRFGAKDVFDLSWRNLLDAYSCTECGRCSAACPANLTGKVLSPRK
                     VMMGTRDRLEEVGRNIDANGKFTDDGKSLLDYIPVEALRACTTCNACVEECPVSISPL
                     EIIIEMRRALIMEDSNAPQEWNALFGNIENNFAPWKFSPDDRDKWIAE"
     gene            complement(2770..3309)
                     /locus_tag="VGQ51_16045"
     CDS             complement(2770..3309)
                     /locus_tag="VGQ51_16045"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV9038148.1"
                     /translation="MMKFLLGGLMLLSAAGCLAQQYKPADQGSEIGFVIKNFGINTKG
                     TFSGLNGNIGWYPRHTDSCLFDVSIDAATVNTDNSMRDDHLKKEAFFDVEKHPRIRFV
                     STSVTGPDRKGHYTITGRLTIKDTTKDISFPFLVTALGDDLIFKGSFTLNRRDFDVGG
                     SSTISNQLTVSLTVLAKKQ"
     gene            complement(3306..4004)
                     /locus_tag="VGQ51_16050"
     CDS             complement(3306..4004)
                     /locus_tag="VGQ51_16050"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV9038149.1"
                     /translation="MAGAEEPEWVRNYDMWCHLPDEEYAALKIEHSVIEVPKGEYVYF
                     EAYKHNKIYFVKEGTIKIGYIDDDGNEKVKEIIRKGELFGQFGLERNNLHGEFAQAYK
                     DDVSICAFNIDDFERLLKSRPDLALKYSKQVGAKLRQIENRLVNLLNKDVKTRLLHFF
                     WQLVEQNLGENTAEGFCIPNFLTHEDIAGLIGSSRQTVTTMINELETEGILSYNRQEI
                     CFLDVKKLQTRIQQ"
     gene            4105..4884
                     /locus_tag="VGQ51_16055"
     CDS             4105..4884
                     /locus_tag="VGQ51_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV9038150.1"
                     /translation="MHVPTMAEFAAKGESPEILFWVGCSGSYDQRAQKITRAFAAILD
                     KVGIRYAVMGKEEICNGDPARRAGNEFLFQMMAYQNIRLLNNYGVKKIVTACPHCFNI
                     FANEYPALGGNYEVVHHTVFLQQLIDEGRIRLKEGGPYKGRRITYHDSCYLGRGNGIY
                     EAPRKVLEALDAELVEMKRCRSKGLCCGAGGAQMFKEEERGSTRVNFERSSEAIGTGA
                     EVIAAACPFCNTMLTDGVKHEEKEDTVKVLDIAELIAASLE"
     gene            4947..5465
                     /locus_tag="VGQ51_16060"
     CDS             4947..5465
                     /locus_tag="VGQ51_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038151.1"
                     /translation="MNAQHTDLIPAGFAADSRVWIYQSPRLFFLSEALVIEEMLETFV
                     REWKSHGTPVKGYANLFYGQFIVLMADETATGVSGCSTDTSVRLIKDIEQRFGTPLFD
                     RLMLAFRIKDKVEMVPISQIPYAIENGFIGPDTQYFNNTVATKAELETRWLIPLKDSW
                     LAARYLKTVPKD"
     gene            5594..5669
                     /locus_tag="VGQ51_16065"
     tRNA            5594..5669
                     /locus_tag="VGQ51_16065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5627..5629,aa:Gly,seq:ccc)
     gene            5792..7213
                     /gene="miaB"
                     /locus_tag="VGQ51_16070"
     CDS             5792..7213
                     /gene="miaB"
                     /locus_tag="VGQ51_16070"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEV9038152.1"
                     /translation="MLESLSSKVHDEERQGEAYRPATTESGKFRKKFYIESYGCQMNF
                     SDSEIVASILQESGYGATSNYTEADLVLLNTCSIREKAEVTVRNRLQLFRQIKEDRPG
                     MLIGVLGCMAERLKGKFLEEEQLVDMVVGPDAYRTLPGLIVEAGGGQKAVNVLLSREE
                     TYADISPVRLDGNGVTAFVSIMRGCNNMCSFCVVPFTRGRERSRDAVSIVRECEDLFR
                     RGFKEVTLLGQNVDSYYWPDEANDRVVDFAHLLEMVARIDPSLRIRFSTSHPKDITDE
                     VLYTMARYDNICRYIHLPVQSGSTRILQLMNRTYTREWYMARIDRILSILPDCGLSTD
                     IIAGFCTETEEDHLDTLGLMEYARYDMSYMFFYSERPGTLAARRYQDDVPEAVKKRRL
                     EEIVRLQNRLSLEKNKQDVGRIYTVLIEGDSKKSAGDWRGRNSQNKMVVFPKNGSDLQ
                     KGDYVEVKIVDCTGATLIGRLAE"
     gene            7223..>7882
                     /locus_tag="VGQ51_16075"
     CDS             7223..>7882
                     /locus_tag="VGQ51_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV9038153.1"
                     /translation="MEIQSIKNRFGIIGNSPGLNFALQVAAQVAVTDLTVLIAGESGV
                     GKEAFSMIIHSLSARKHNPFIAVNCGAIPEGTIDSELFGHEKGSFTGALDSRKGYFET
                     VNGGTIFLDEIGELPLGTQARLLRVLEAGEFIRVGSSKVQKTDVRVIAASNKDLLELT
                     EKAKFREDLYYRLSTVPIRVPALRDRKEDIPLLFRKFAVDFAERYKTAPVQLDEEAKN
                     LL"
BASE COUNT         1808 a         2077 c         2136 g         1861 t
ORIGIN      
        1 aagctcttcg atcgaaatat cgcgtttgtc gatataggcc acgcagccgt cgatacactc
       61 cgacaggttg tgcggcatca tattggtggc catcccgacc gcaatgccgc tggagccgtt
      121 catcagcaac tgaggcaggc gagtggggag taccgtcggt tcctgcagtg aatcgtcgaa
      181 attcggctgg aaatccacgg tatccttctc gatgtcctcc agcatgtact cggcctgacg
      241 atggagcctt gcctcgatat accgcattgc cgcagggcca tcgccgtcct ggttaccgaa
      301 attaccctgg ccatccacaa gcatataacg catgctccat tcctgcgcca tgcggaccat
      361 ggcatcgaaa atggaggaat cgccatgagg gtgatatttt cccatcacgt ccccgacgat
      421 acgggccgct ttcttatacg gcttgttact gtgattgcct aattcattca tgccgaagag
      481 gatacggcga tgcaccggct tcagtccgtc ccggacatcc ggaagggcac ggctcacaat
      541 gacggacatc gagtaatcga tataggccgt cttcatctgc tcttcgatat ttacagggac
      601 aatgcgattg ttatcctgcg tctcagtcgg agtattatta tcatccataa caatgatcac
      661 acccaaacgg accaccggtc cgtcggcccg aaggcccaat aattggttaa tcgggggatg
      721 gcaaatttac cccttttggc ctgaaaatcc gctgttagcg gccttttttt aacggatatt
      781 ttccactgga tgtggataca atgccacctg gtcaggccgc ccccgaagat tccggccggc
      841 atgccgcgaa ccgctattcc agccaggctc ccctgacctg gtcgccgtcc acttccacat
      901 aaatatgctc ctgcagggtg gtggatagtg acaatccggt atagtcaggt tgtatcggga
      961 aggctttatg cgatcgttcc accagtacca gacattcgat cttccgggga tgggcctcta
     1021 aaaatggttt catcgcatac aaaagggtct tgccgctgtt ggcgacatcg tcgatgacga
     1081 cgatgacctg cccgtcgaag gaaagcgatt cgctcagcgt aaccgggccc ggttcttttt
     1141 tgtcgagggt aatggtgatc agccgggtcc gcaggcggtc gccggctatc tcctgtaaaa
     1201 gacgctggat attccgggcg atgacgctcc cgctttccct gatccccgcc aggatcagct
     1261 gttcttcgcc ggagttgttc tccaatagct cataagccat gcggcgtagt ttcttgtccg
     1321 ccacggcttg cgtaagaata tagtttttca tgtcgcaccc aaattacgct attacgtcaa
     1381 atcacctatc ttagttccat taataatttt tctatggccc tttcgcagca gatcgtattc
     1441 ctcctggtcc tggcaacagc cattatcctg tttaccaaaa agatcagatg ggtcagacgg
     1501 aatatcctgc tgggtaagga tgaagactat tccggacaat cgggacgccg gtggaagaat
     1561 gtgctcctgc ttgcattcgg acagaaaaaa atgttccgga acccgatggt cgccgtcatg
     1621 catttcgtcg tctatgccgg cttcatcatc atcaatctgg aagtgctgga gatcgtcctg
     1681 gatggaatcc tgggaacgca tcgtctgttc tattttgccg gtcctttata cctgggcctg
     1741 atcgatgcct tcgagatcct tgcagcaggc gttttcctgg cctgctttat tttcttctgc
     1801 cggcgaaatg tggtgaagct gcgccggttc atcagtcatg acctcgatgg atggccgcga
     1861 agcgacgcta attacatcct gctgaccgaa atggcgctca tggccctctt cctcaccatg
     1921 aacgcggccg atacgctgct ccagtcccgt ggatatgacc attatgccgc acatatcacg
     1981 ggggattttt ggttttcaca gtggctgcat cctttgctca gcggctggag caacggggcg
     2041 ctggtcgtcc tggaacgcac ctgctggtgg ttgcatattc tcgggatcct ggcctttctc
     2101 aactacctgc cctattccaa gcacctgcat atcctcctgg cgtttccgaa tgcgtattat
     2161 gcccggctgc aaccggaggg gcgtatccga aacatgcctt ccgttcaaaa cgaggtgctg
     2221 tatgcgatgc aacccgaaac tgcgcctccg gctcctgccg acggtagcgt cccccccgcc
     2281 ccccgattcg gagccaaaga tgtcttcgat ctgagctggc gcaatctgct ggatgcctat
     2341 tcctgcacgg aatgcggacg ctgctccgcc gcctgtcccg ccaacctcac ggggaaggtg
     2401 ttgtccccgc gcaaggtcat gatgggcacg cgcgacaggc tcgaagaagt gggtcggaac
     2461 atcgatgcca atggcaaatt caccgatgac ggaaagtcat tgctcgacta tattcccgtc
     2521 gaagcgctga gggcctgcac gacctgcaac gcgtgcgtgg aagaatgccc cgtcagtatc
     2581 agtccactgg agatcattat cgaaatgaga agggcgctca tcatggagga cagcaatgcg
     2641 ccgcaggaat ggaatgccct gttcggcaat atcgaaaaca atttcgctcc ctggaaattc
     2701 agtccggacg accgggacaa atggatcgcg gagtgaggct cccgccggtg gccgaagggt
     2761 gagccccgcc tattgctttt tcgccagcac ggtcagcgat accgtcagct gattggagat
     2821 cgtgctcgat ccgcccacat cgaaatcgcg tctgttcagg gtgaagcttc ccttgaaaat
     2881 cagatcatcc cccaatgcgg ttacaagaaa aggaaaagag atatcttttg tcgtatcctt
     2941 gatcgtcagc ctgccggtga tcgtataatg ccccttcctg tcggggcccg ttacgcttgt
     3001 agaaacaaac cggatccgcg gatgtttttc cacatcgaaa aaagcctcct ttttgagatg
     3061 gtcgtcccgc atcgaattat cggtattcac ggtagcggcg tcgatgctga cgtcgaaaag
     3121 acaggagtcc gtatgccgcg gataccagcc aatgttgccg ttcaggccgc tgaatgtccc
     3181 cttggtattg atgccgaaat ttttgatcac gaacccgatc tccgaaccct gatcggcagg
     3241 tttgtattgt tgcgccagac agccggccgc agacaggagc atcaggcctc ctaaaagaaa
     3301 cttcatcatt gttggatacg tgtttgcaat ttttttacgt ccaggaaaca tatctcctgc
     3361 cgattatagg agaggatgcc ttccgtctcc agttcgttga tcatggttgt caccgtttgc
     3421 cggctgctgc cgatgagacc ggcgatgtcc tcatgtgtaa ggaaattggg aatacagaaa
     3481 ccttccgcgg tattctcgcc caggttctgt tccaccagct gccagaagaa atgcaggagg
     3541 cgggtcttca cgtctttatt gagcagattg acaagcctgt tctcgatctg tctaagcttt
     3601 gcacccacct gtttgctgta tttcagcgcg aggtcgggtc tgctcttcag caggcgctcg
     3661 aaatcgtcta tattgaacgc acaaatgctc acgtcgtctt tataagcctg ggcgaattcg
     3721 ccgtggaggt tattgcgctc cagaccgaat tgcccgaaaa gctcaccctt ccggatgatc
     3781 tctttgacct tttcattgcc gtcatcgtcg atatacccga tctttatcgt tccctctttg
     3841 acaaaataga tcttgttgtg cttgtacgcc tcgaaataaa catattcacc cttcggcact
     3901 tcgatcaccg aatgctcgat cttcagcgcg gcgtactctt catcgggtaa atggcaccac
     3961 atatcatagt tgcgtaccca ttccggttct tcagcacctg ccatattttg cggtttagcg
     4021 gctaaagtta ggggtggatc atcgctttcc aaaataaatt gcgatgaatg aagtgtttgg
     4081 ctagatttac ggaaaattac ccgcatgcac gttccgacga tggccgaatt cgccgctaaa
     4141 ggagagagtc cggagatcct tttctgggta ggatgctcgg gcagctatga tcagcgcgcg
     4201 caaaaaataa caagggcatt cgccgccatc ctggataaag taggcatccg ttatgccgtg
     4261 atgggcaaag aggagatctg caatggcgat cccgccagga gagccggcaa tgaattcctg
     4321 ttccagatga tggcctacca gaatatccgg ttgctgaaca attatggtgt aaaaaagatc
     4381 gtaaccgcct gcccgcattg cttcaatatc ttcgccaatg agtatcctgc tttgggtgga
     4441 aattacgagg tggttcatca taccgtgttt ttgcagcagc tgatcgacga agggcggata
     4501 aggctgaaag agggcggccc gtataaaggc cggcggatca cctatcacga cagctgttat
     4561 ctcggccggg gtaatggcat ctatgaggcg ccacgcaaag tgctggaagc cctcgatgcc
     4621 gagctcgtgg aaatgaaacg ttgccgcagc aaaggtctgt gttgcggcgc aggaggagcg
     4681 caaatgttca aggaagagga acgcggatcg acccgcgtga atttcgaacg cagcagtgaa
     4741 gcgatcggca ctggcgcgga ggtcatcgcg gcggcctgtc cattttgtaa taccatgctg
     4801 acggatggcg tcaagcacga agaaaaagag gacaccgtaa aggtgctcga tatcgcggag
     4861 ctgatcgcgg cttcgctcga atagcgtgcg cccggcggtc gcccctgccc tatacggcgc
     4921 ccgctccaat tttgtatctt tgcgtcatga atgcacaaca caccgatctg atcccggccg
     4981 gcttcgcggc ggactcgcgt gtctggatct atcagagccc ccgtctgttc tttttatcgg
     5041 aggccctggt catcgaagag atgctggaaa ccttcgtccg cgaatggaag tctcatggta
     5101 cccccgtcaa aggctacgcc aatctgttct atggtcaatt catcgtgctg atggccgacg
     5161 agaccgccac cggcgtcagc ggatgcagca ccgacacctc ggtccgtctg ataaaagata
     5221 tcgaacaacg tttcggcaca ccgctgttcg accggctgat gctggccttc cggatcaagg
     5281 acaaggtcga gatggtaccg atttcgcaaa taccttatgc catcgaaaat ggattcatcg
     5341 gtccagatac gcaatatttc aacaacaccg tcgccacaaa agccgaactg gagacccgct
     5401 ggctgatccc tttgaaagat agctggttgg ctgcgcggta tctgaagact gttccgaagg
     5461 attgaaaaat atttttatca aaagacggca agcccttatt tttgtccccc ggccgcaacg
     5521 tcgtctttga cgaagttcag cggcaccggg acgaaccacc ggttcgccgg caaaaaggcc
     5581 agacattttt tccgcggaag tagctcagtt ggtagagcga cagcttccca agctgtaggt
     5641 cgcgggttcg aacctcgtct tccgctccaa tcgtcagatt caaaggccac ttcgtcgggt
     5701 ggctttttta ttctgatgca ggaagcgctg ccaaattgtc acgaccaatt ccttttctgc
     5761 ttatctttgc cgtctaaaac ttgaaagaga aatgcttgaa tcgttgtcca gcaaggttca
     5821 cgatgaagaa aggcaaggag aggcttatcg acctgccaca acggaatccg ggaagttccg
     5881 gaaaaagttc tatatcgaaa gttatggctg ccagatgaac ttttctgaca gcgagatcgt
     5941 cgcctccatc ctgcaggaaa gcggttatgg tgcgacaagc aattatacag aagccgatct
     6001 ggtcctgctg aatacctgta gcatccgcga gaaggcggaa gtcacggtcc gcaacaggct
     6061 gcagctcttc cgtcagataa aggaagaccg gcccggaatg ctgatcgggg tgctggggtg
     6121 catggccgag cggctgaaag ggaaattcct cgaagaagag caattggtcg atatggtcgt
     6181 cggtcccgat gcgtaccgga ccttgcccgg gttgatcgtc gaggccggag gcggccagaa
     6241 ggcggtgaat gtattgctca gccgggaaga aacttatgcg gacatttccc ccgtgagact
     6301 ggatggcaat ggggtaacgg ccttcgtcag catcatgcgc ggctgcaaca atatgtgctc
     6361 gttttgtgta gtaccgttta ccaggggccg cgaacgcagc cgggatgccg tttcaatcgt
     6421 cagggaatgc gaggatcttt tcagaagagg gttcaaagag gtgacgctac tcgggcagaa
     6481 tgtcgacagt tattattggc cggatgaggc taatgaccgg gttgtcgatt tcgctcatct
     6541 tctcgaaatg gtcgccagga tcgatccgtc gctgaggatc cggttctcga cctcgcaccc
     6601 aaaagatatc accgatgagg tattgtatac catggcgcgt tatgacaata tctgccggta
     6661 tatccatctg cccgttcaga gcggctctac ccgcatcctg cagctgatga accgcactta
     6721 tacccgcgaa tggtatatgg ccaggatcga ccgcatcctt tccattcttc cggactgcgg
     6781 gttgagcacc gatatcattg cgggtttctg taccgagacg gaagaagacc atctcgacac
     6841 gctgggcctg atggagtacg cgcgttatga catgagctat atgttcttct atagcgaacg
     6901 gccgggtacg ctggccgcgc gccgctacca ggacgacgta ccggaagccg taaaaaaaag
     6961 aagactcgaa gagatcgttc gtttgcagaa ccggctttcc ctggaaaaga acaagcagga
     7021 tgtaggcagg atatataccg tgctcatcga aggagattcg aaaaaaagcg ccggcgactg
     7081 gcggggacgc aatagccaga ataaaatggt ggtattcccg aaaaatggat cggaccttca
     7141 aaaaggagat tatgtagaag tgaagatcgt cgactgtacg ggtgcgacac tgatcggccg
     7201 gttggcggaa taaatgatgt ttatggaaat tcaatcaatc aagaaccgtt ttggcattat
     7261 aggcaattct ccgggactta acttcgccct gcaggttgcc gcacaggtgg cagttaccga
     7321 cctgacagtg ctgatcgccg gggagagcgg ggtgggaaaa gaagcctttt cgatgatcat
     7381 ccattccttg tcggcccgca aacataatcc ctttatcgcg gtgaactgcg gcgccatccc
     7441 cgaggggacc atcgattctg aacttttcgg acatgaaaaa gggtcgttca ccggtgcatt
     7501 ggattcgcgg aagggatatt tcgagacggt gaacgggggt acgatctttc tggatgagat
     7561 cggcgaattg cccctgggca cgcaggcccg tttgctgaga gtgctggagg ccggagaatt
     7621 catcagggtc gggtcctcca aggtgcagaa gacggatgtg cgcgtgatcg ccgcatcgaa
     7681 caaggacctg ctggaactga cggaaaaggc taaattcagg gaagatctct attaccggct
     7741 gagcaccgta ccgatccggg tgccggcatt gagggacagg aaggaggata ttccgttgct
     7801 attccgtaag ttcgccgtag actttgccga acggtacaag acggcgcctg tgcagctgga
     7861 tgaggaggcg aaaaatctgt ta
//
LOCUS       DASYSV010000517         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2285648, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000517 DASYSV010000000
VERSION     DASYSV010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285648"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VGQ51_16080"
     CDS             complement(<1..385)
                     /locus_tag="VGQ51_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038154.1"
                     /translation="MLKNLLLFSVAASMSCGALAQSIAGDQTARSTRPATPANPANPP
                     NWVDKAGDPVDYVNPLMGTQSTYELSSGNTYPSIGLPWGMNFWTPQTGKNGDGWQYTY
                     TVNKIRGLKQTHQPSPWMNDYGEFSI"
     gene            complement(379..1260)
                     /locus_tag="VGQ51_16085"
     CDS             complement(379..1260)
                     /locus_tag="VGQ51_16085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEV9038155.1"
                     /translation="MSRYIQMTLVLFYVLLTIFAFLTVLVVSGLHLSNTRGWWIVAGW
                     VALCYGSASVRAGVRTFLFSNYRKPVLREEQKLHPAMEELLRQAGTNRRVRVWIEASG
                     GYNAYATGLHTIAITDGLLAALSPSELRGVLAHELGHLLSGDTIGAAAFSTAGLLPQG
                     VFRIFRIGAGIVRGAFAGMGTLLGGIIVILVLGYGLYRIHLLNAVVPVILFVLFFGIL
                     NRIFYFFRLWLMRLAEYRQDAFAFRMGHGEGLVRALAKLAATDEPIVNRYYIVMHSSH
                     PVIHNRIRRLEKMNQTC"
     gene            1329..>2371
                     /locus_tag="VGQ51_16090"
     CDS             1329..>2371
                     /locus_tag="VGQ51_16090"
                     /inference="COORDINATES: protein motif:HMM:NF037611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEV9038156.1"
                     /translation="MKRSISLLFSFAVYTLSAQQRPKESAPLSFVDPYIGTAAHGHVF
                     LGASVPFGAVQVGPTNINKGWDWCSGYHYSDSVVKGFSQDHLNGTGIPDLCDVLVMPF
                     TGRVRTEPGTQNDPASGYSSHYDHAHEIARPAYYYVYLKEHHVGVELTASERVAFHRY
                     SFPKDRPGHIIIDLFEGNFDKSWQHGKIYGSLKKLDDSTLIGYRNSSQWAQDRRIYFA
                     IRTSVPLKDFELLHGDRRMDGDSLEADSVKALFAVPSPSTVMLKIGLSNVSGENALAN
                     IEAEIPGWDFEDIVRQGNEKWERQLQKIVIQPADASQEKVFYTALYHTLISPALYNDH
                     DSSYRGVDGKVVE"
BASE COUNT          560 a          651 c          646 g          514 t
ORIGIN      
        1 tgatgctgaa ttcgccatag tcgttcatcc agggagaagg ttgatgcgtc tgtttgagac
       61 cgcggatctt gttgacggta taggtgtatt gccagccgtc tccgttctta cccgtctgcg
      121 gggtccagaa attcatgccc caggggagac ctatcgacgg ataggtattg ccggaagata
      181 gttcataagt ggattgggtt cccatcaatg gattgacata atcgacggga tcaccggcct
      241 tgtcgaccca attggggggg ttggcggggt tggcgggagt ggcgggtcgg gtggacctgg
      301 ccgtctgatc accggctatg gactgggcga gcgctccgca ggacatcgag gcggcaacgc
      361 taaaaagcag cagatttttt agcatgtttg gttcattttc tccagccgcc tgatccggtt
      421 gtggatcacc gggtggctgc tgtgcataac gatataataa cgattgacga tcggttcatc
      481 cgtcgcggcc agctttgcca atgccctcac cagcccttct ccatgaccca tccgaaaggc
      541 gaaggcatcc tgcctgtatt cggccagccg catcagccaa agccggaaga aataaaaaat
      601 gcggttcaag ataccgaaaa acaggacgaa aaggatcacc ggtactaccg cattcaacag
      661 gtggatgcgg tacagtccat aacccaacac caaaattacg atgatgccgc ccaggagagt
      721 acccataccg gcaaacgctc cgcgaacgat gccagcgccg atccgaaaga tccggaacac
      781 gccctgggga agcaatcctg ccgtagaaaa ggctgcggcc ccgatcgtgt cgccgctaag
      841 cagatgcccg agctcatgag cgagcacacc ccgtagctcg gaaggagaca gagctgccag
      901 caatccgtcc gtaatggcta tcgtatgcag acccgtcgcg taggcattgt acccccccga
      961 ggcctcgatc catacccgta ccctacggtt ggtccctgcc tgccgaagca gctcttccat
     1021 cgcgggatgt agtttttgtt cttcgcggag gaccggtttc cgataattcg aaaaaagaaa
     1081 tgtccgcact ccggcccgca cagacgccga tccgtaacac aaggctaccc agcctgcgac
     1141 gatccaccat ccgcgggtat tggacagatg cagacccgac acgaccagca cggtcaaaaa
     1201 agcaaagatg gtcagcagca catagaacag caccagcgtc atctgtatat acctgctcat
     1261 aaaggttaag gttatgaagc ttcgaattta cccgcatttt accacttctt cttttttatc
     1321 tttgatccat gaaaagatcg ataagccttc ttttttcttt cgccgtctac acgctatccg
     1381 cgcagcaacg tccaaaagaa agcgctccgc tttccttcgt cgacccctat atcggtaccg
     1441 cggcgcacgg tcatgttttc ctgggagcca gcgtgccctt cggcgccgtg caggtggggc
     1501 ccaccaatat caacaaggga tgggactggt gttccggtta ccattattcc gacagcgtcg
     1561 tgaagggttt ttcgcaggat catcttaacg gtaccggtat accggatctg tgcgatgtac
     1621 tggttatgcc gtttacggga cgggtgagga cggagccggg tacccagaac gaccctgcat
     1681 cggggtactc ctcgcattat gaccatgccc atgagatcgc gcggccggcg tattattatg
     1741 tttacctgaa ggaacatcat gtaggtgtcg agctcactgc aagcgagcgg gtggcattcc
     1801 atcgttattc gtttccgaaa gaccgtcccg ggcatatcat tatcgatctg ttcgaaggga
     1861 atttcgacaa gagctggcag cacgggaaga tctatggatc cctgaaaaag ctggatgact
     1921 ccacgctaat cggttatcgc aattcgagcc agtgggcgca ggacaggagg atctattttg
     1981 ctatacggac cagcgtcccg ctaaaggatt tcgagctgct gcatggcgac aggcggatgg
     2041 atggcgattc gctggaagcc gatagcgtaa aggcgctgtt cgccgtgcca tcaccttcca
     2101 ccgtcatgct caagatcggc ctttccaatg tcagcggtga gaatgcgctt gcaaatatcg
     2161 aggccgaaat acccggctgg gactttgagg acatcgttcg tcagggcaat gaaaaatggg
     2221 aacggcagtt gcaaaagatc gtcattcaac cggccgacgc gagccaggaa aaggttttct
     2281 acaccgcctt gtatcacacg ctgatctctc ccgccctgta caacgatcac gacagcagct
     2341 atcgcggcgt cgacggcaag gtcgtagaac a
//
LOCUS       DASYSV010000518         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1638360, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000518 DASYSV010000000
VERSION     DASYSV010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638360"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VGQ51_16095"
     CDS             complement(<1..220)
                     /locus_tag="VGQ51_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038157.1"
                     /translation="MLRNYFLTAWRNLVRNKAYSTLNILGLSIGMTVALLIGLWVQYQ
                     YSYDRSLPNYRQVYQARGRFSRNGQKEQM"
     gene            complement(394..>1541)
                     /locus_tag="VGQ51_16100"
     CDS             complement(394..>1541)
                     /locus_tag="VGQ51_16100"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEV9038158.1"
                     /translation="LDRKSLNVRGLRPITFDPATDIVFLYSETLPYHPNAMTFAADWE
                     DGYQLAETWYSIGGGFVRKEVQEASVGSAMQLPFPIDTNADLLHWCRMTGLSISEVVL
                     ENEAAWRSEAETRSGVGLIWKVMRECVYRGCHAEGMLPGGLHVQRRAAMLNRKLIGDR
                     PYHDFESWVAAIRSGGNGFKYTLDWVSCFALAVNEENASFGRVVTAPTNGAAGVIPAV
                     LQYFVIFCDGLEEEKIFRFILTASEIGSIFKKGATISAAMGGCQAEIGVSSAMAAAAL
                     TESVGGNQRQAMMAAEIAMEHHLGLTCDPIGGLVQIPCIERNTMGAIKAITASQLALQ
                     STPDFAKVSLDAVIQTMWHTAQDMNSKYKETSDGGLAVQIPLGLSEC"
BASE COUNT          329 a          430 c          446 g          336 t
ORIGIN      
        1 ccatctgttc tttttggcca ttgcgggaaa atcgaccacg agcctggtaa acctgccggt
       61 aattggggag cgatcggtcg taggagtatt ggtactgaac ccagagaccg atcagcaggg
      121 ccacggtcat accgatggag agaccgagga tattcagggt gctatatgct ttattgcgga
      181 cgaggtttcg ccaggcagtg aggaaatagt tgcggagcat gggaacgagt taattttgga
      241 ggaaagaggc caataggatg ccgaaatcac agcaacctgt gcctcatcgt tttagttccg
      301 cgatctgttc tggctctgtc cggtttcgat acacgggatg tgcgttcaac attcgctcaa
      361 ccccagcggt atctgcacgg cgagcccgtt cgctcaacat tcgctcaacc ccagcggtat
      421 ctgcacggcg agcccgccgt cggaggtttc cttgtatttg ctgttcatgt cctgcgcggt
      481 atgccacatc gtttggatga cggcatcgag cgacaccttc gcaaaatccg gggtgctttg
      541 caacgccagc tgcgatgcgg taatggcttt tatcgcgccc atcgtattgc gttcgataca
      601 gggtatctgc acgagaccgc cgatgggatc gcaggtgagc ccgagatgat gctccatcgc
      661 gatctccgcg gccatcattg cctgccgctg gttgccccct acggattcgg tgagggccgc
      721 ggcagccatg gcgctactta cccctatctc cgcctgacaa ccgcccatgg cggcggaaat
      781 ggtggcgccc tttttaaaga tgctgcctat ttccgaagcg gtaaggatga aacggaagat
      841 cttttcctct tcgagaccgt cgcagaaaat gacgaagtat tgaaggaccg cggggatgac
      901 gccggccgcg ccgttggtgg gagcggtgac gacacggcca aaagaggcgt tctcttcgtt
      961 gacggcgagg gcaaaacagc tgacccagtc cagcgtatat ttgaaaccgt tgcctcccga
     1021 ccggatagct gccacccagg attcaaaatc gtgatatggg cggtcgccga tcaattttcg
     1081 gttcagcatg gcggcgcgac gttgcacatg aagaccaccc gggagcatgc cttctgcgtg
     1141 acaaccccga tagacgcatt cccgcataac cttccatatc aggccgacac ccgatcgcgt
     1201 ctcggcctcg gatctccatg ccgcctcgtt ctccaggacg acctcgctga tgctcaaccc
     1261 ggtcatgcgg caccaatgaa gcagatcggc attcgtatcg atggggaaag gcagctgcat
     1321 ggcagatcct accgacgcct cctgaacttc tttgcggacg aatcctccgc cgatggaata
     1381 ccaggtctcg gccagttgat aaccatcttc ccaatcggcc gcgaatgtca tggcgttggg
     1441 atgatagggt aaggtttcgg aataaagaaa aacgatatcg gttgccggat cgaacgtgat
     1501 gggccggagg ccgcggacgt tcaggctttt ccggtccagg a
//
LOCUS       DASYSV010000519         8017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1638386, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000519 DASYSV010000000
VERSION     DASYSV010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638386"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGQ51_16105"
     CDS             <1..415
                     /locus_tag="VGQ51_16105"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEV9038159.1"
                     /translation="FSLTGIAGLYFWLDLEFIAAVQVVVYVGGIVVLIIFSIFLTQQA
                     GKDLPRPLRTRAFFSASAVCIGFLLTSLMIRQYGFPLPPAGGKTDMGIATIGTQLLST
                     SGQGYALPFEVISILLLAAMIACIVIAIKIKPGEK"
     gene            412..726
                     /gene="nuoK"
                     /locus_tag="VGQ51_16110"
     CDS             412..726
                     /gene="nuoK"
                     /locus_tag="VGQ51_16110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEV9038160.1"
                     /translation="MSQVPLTHILFVSTALFFIGMYGLFTRRNMITMLMSVELILNSV
                     NINFVAFNKYLYPGALEGVFFTLFIIAIAAAEAAVAIAIIINLYRNFRSIDVEDADEL
                     KF"
     gene            738..2642
                     /gene="nuoL"
                     /locus_tag="VGQ51_16115"
     CDS             738..2642
                     /gene="nuoL"
                     /locus_tag="VGQ51_16115"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEV9038161.1"
                     /translation="MPNAAYIVLIPLLPLVGFVLLGLFGKKFFRKSSGIIGTGLVLVS
                     AALAIWVAYGYFFEWGTDGASLKVLDMRWLAFSGGLSIDMNIVLQPLPVIMIVVVSFI
                     SLMVHLFSLGYMKGEPRFATYYAYLGLFTFSMLGLVLAGNLFEIYIFWELVGCSSYLL
                     IGFYYDRPTAVAAAKKAFIVTRFADLGFLVGILILSFQGRTLDMDNLLQTLFSPAFQA
                     SGVSFLGMSVVAWGATLVFIGGAGKSALFPLHIWLPDAMEGPTPVSALIHAATMVVAG
                     VYLVARLYLLYALNGVVPQIVLYTGAFSALLAAIIACTQTDIKRVLAYSTMSQIGYMM
                     FALGTFRPAGTDFLGYTASIFHLFTHAFFKALLFLCAGVIIHRVHSNEMKDMGGLRKA
                     MPLTHICFLVACLAISGIPPFSGFFSKEEILLAAWRANKFVYAMGLFTSGLTAFYMFR
                     LYFAVFWHRPVTDGHRHPMPFFESIPLVILALGAAFAGFIPFSRFILPGAPERAGGGI
                     GVFSIAPVGIALVGIGIAAWLYRKESDRPQRVATSMRGLYTFAYHKFYIDEVYLFVTK
                     KIIFNLVGRPSAWIDRNIVDGLMNGMAATTARISAGIKGVQSGKVQDYTWFFFIGIAG
                     LAVIFIYIYT"
     gene            2639..4075
                     /locus_tag="VGQ51_16120"
     CDS             2639..4075
                     /locus_tag="VGQ51_16120"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEV9038162.1"
                     /translation="MNLSLLIIAPLVTAVAVLFCRGLSGIRWVSLAGVTVQLALSLVL
                     AVLYEKERGAGESAHWLFQYWHAWFIPLNIAYHVGVDGLSVAMILLTAFVMLAAVLVS
                     WRIDRMGKEFFFLLLVLSAGAYGFFISLDIFTLFFFLEISVIPKFLLIGIWGSGRKEY
                     SAMKLALMLMGGSALVLVGLLGIYTSGAYTFDLPALGGNLSLHAQRLFFPFLFVGFGI
                     FTALFPFHTWVPDGHSSAPTAGSMFLAGVSMKLGGYGCLRMALLMPAVAREYSPYIIV
                     LSVIAIVYGAFATMMQNDLKYINAYSSVSHCGFILLGIGMLTETAVRGAVLQMISHGL
                     MTALFFATIGMIYDRTHTRLVARLGGLLKVMPFISTVFVIAGLCSLGLPGLSGFVAEV
                     TVFMGSWQHPGQMYHLATILACASIVITAVYILRATGATVIGPVGDSQYYSLKDATWT
                     ERLAGILLVTGIIAIGVAPFWLNELIAK"
     gene            4086..5498
                     /locus_tag="VGQ51_16125"
     CDS             4086..5498
                     /locus_tag="VGQ51_16125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEV9038163.1"
                     /translation="MWTDILFSLRQELVVTAILFLLLFIKVGASEYRTETLLWLINIL
                     LAVNFILGFLPGGSSVLFSGMFHTNGMIVLEKNILNLGTLIVSLLAYDWLKDHRHVAE
                     FYMLLLSTLLGFFFMISSGHLLLFYLGLELSTIPLTALSSFDLEKRQSSEAAMKFIIS
                     SAFSSALLLFGISLLYGATGTLSFDTIPRHLDGSAFQLFAFILILSGFAFKISVVPFH
                     LWTADVYEGSPVPVTAYLSVISKGGVFFIFVQTLYTVFEPMGDNWYRIVVLLSVVTML
                     TGNLFALRQNNFKRLLAFSSIAQSGFILAGISGSSRSGDASVIYFILIYVFSNLGAFG
                     VVSLVSAATGKENIDDYKGFYATNPVLSWVMAISLFSLAGIPPTAGFFGKFFLLMAGA
                     GKHNTIFILIAALNMVISLYYYLRVIKVIFMDSQPQPIRKLEIPLLPNIAFILCVTGV
                     LLTGVMGWVYDYIYSWSIGT"
     gene            5500..5889
                     /locus_tag="VGQ51_16130"
     CDS             5500..5889
                     /locus_tag="VGQ51_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038164.1"
                     /translation="MAINQNHTFEELNGVKCGIVEKTVTPARVEFLRGILEYNHYTVV
                     VAPSPPPKTAPATAPAEAAASSPAGAEPPTAPGTFTVGVTDVTFNPVNAIFGRLLRTP
                     EGHVVTLAYWQQKDAASNDTIPYFTTE"
     gene            5886..6605
                     /locus_tag="VGQ51_16135"
     CDS             5886..6605
                     /locus_tag="VGQ51_16135"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV9038165.1"
                     /translation="MKLSIVDSPGSMAADVADRVKECLRFTSRPLFCPASGDTPAALY
                     AELLRRAMDHTMNMREWSFVGLDEWVGMNGEDAGSCRYYIDRGLFHPLNVPADRICFF
                     DGRAPDLQKECADVERFIGRWGRIDVAILGIGPNGHVAMNEPGSDPAGRSQVSELHPA
                     TRETGQKYFSCHQTLERGITLGLGTLMEARHLFLLAIGEKKAEIIRKALEGPITTELP
                     ASLLRNHPGLEVWLDAGAAGR"
     gene            complement(6824..7324)
                     /locus_tag="VGQ51_16140"
     CDS             complement(6824..7324)
                     /locus_tag="VGQ51_16140"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEV9038166.1"
                     /translation="MKTQPIRRNKNIVSLSHDHHAGLLFCWKLRQGLKHRTEPGRILA
                     YIHYFWQHHLLPHFSEEEAFLFILPDDPGIARAKDEHQRIGVLVDSILTGPSEGILRL
                     LPVLADAVDNHIRFEERDLFPYLEKQLSEAQLESIGRHINENPHLPDDWRDEYWKVTT
                     HSDTLK"
     gene            complement(7335..7691)
                     /locus_tag="VGQ51_16145"
     CDS             complement(7335..7691)
                     /locus_tag="VGQ51_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038167.1"
                     /translation="MKATVFPIHLPASLIEKKSCLIQSLTNWVRTREPIWEQNRLGIA
                     AAGILIQVTFAGAMILILGEAGASPLIFTFGILMAFLADSLAFAQAPMRWLLAIFVLS
                     SGVNLVLSIIYGLMLL"
     gene            complement(7751..>8017)
                     /locus_tag="VGQ51_16150"
     CDS             complement(7751..>8017)
                     /locus_tag="VGQ51_16150"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV9038168.1"
                     /translation="RQLQYKTMMLEEIANEEGEHRLLTLIHYLMARINPENNVLDITK
                     QQLADMTGLRVETVIKTLKRIEKKGRIATQRGKIVCLSNHQPPL"
BASE COUNT         1619 a         1973 c         2263 g         2162 t
ORIGIN      
        1 gttctcgctg accggcattg cgggacttta tttctggctg gacctggaat tcatcgcggc
       61 ggtgcaggtg gtcgtctatg tgggcgggat cgtggtgttg atcatctttt ccatcttcct
      121 tacacagcag gcgggcaaag accttccgcg accgcttcga accagggcat ttttctccgc
      181 atcggcggtt tgcatcggtt ttttacttac aagtctgatg atacgtcaat atggatttcc
      241 tttgccaccg gctggcggta agaccgacat ggggattgca accatcggca cacaactgct
      301 cagcacctcc ggacaaggat atgccctccc attcgaagtg atcagcatcc tgctgctggc
      361 ggccatgatc gcctgtatcg tcattgccat caaaattaaa cccggagaaa aatgagccag
      421 gtccccttga cccatatcct cttcgtcagc acggcactct ttttcatagg aatgtacggg
      481 ctgttcacca ggaggaacat gatcacgatg ctgatgtcgg tggaactgat cctgaacagc
      541 gtgaatatca atttcgtcgc tttcaataaa tacttatacc ccggggcgct ggaaggggta
      601 ttctttactt tgttcatcat tgctatagcg gccgcggaag cggccgtggc aatcgccatt
      661 attatcaatt tatatcggaa ttttcggtcg atcgatgtgg aggatgcgga tgaattgaaa
      721 ttctgaaaaa ctgtattatg ccaaacgctg cttacatcgt cctcattccc ctgttgccat
      781 tggtgggttt tgtgctgctg gggctgttcg ggaagaaatt tttccggaaa tcctccggca
      841 tcatcgggac ggggctcgtg ctcgtgtcgg cggcgctggc aatttgggtg gcatacggat
      901 atttttttga gtggggcacg gacggagctt cattgaaggt gctggatatg cgttggctgg
      961 cattctccgg gggactttcc atcgatatga atatcgtcct ccagccgctg ccggttatca
     1021 tgatcgtcgt ggtgagcttt atctcgctga tggtgcattt gttcagcctg ggatatatga
     1081 aaggagagcc gcggtttgcg acctattacg cttatctcgg gctgttcaca ttttcgatgc
     1141 tcggtcttgt gctggccggc aatctcttcg agatatatat cttctgggaa ctggtcggtt
     1201 gttcttccta tctgctcatc gggttttatt atgaccggcc gaccgccgtc gcggcggcca
     1261 aaaaggcgtt catcgttaca cggtttgcgg atctgggttt tttggttggc attctgatcc
     1321 tgtcctttca gggacgaaca ctggacatgg ataatttact gcagacattg ttcagcccgg
     1381 cgtttcaggc gtcaggcgtc tcgtttttag gaatgtccgt ggtcgcctgg ggggcgacac
     1441 tcgttttcat cgggggtgcc gggaaatcag cgttgttccc gttgcacatc tggctgcctg
     1501 acgcaatgga agggcctacc ccagtatccg cgctcatcca tgccgccacg atggtggtgg
     1561 caggcgtata cctggttgca agactgtatc tcctgtatgc attgaatggc gtggtcccgc
     1621 agatcgtcct gtacacgggg gcgttttcgg ccttgctcgc tgccatcata gcctgtacgc
     1681 aaacagatat caaacgggta ctggcctatt ccacgatgtc gcaaataggc tatatgatgt
     1741 tcgcgctcgg gacttttagg ccggccggaa cggatttcct gggttatacc gcttcgatct
     1801 ttcatctctt cacgcatgcg ttcttcaagg cgctgttgtt cctctgtgcc ggggtcatca
     1861 tccaccgcgt gcatagcaat gaaatgaagg atatgggcgg actccgcaag gcaatgccgt
     1921 tgacgcatat ctgtttcctg gtggcctgtc tggcgatatc ggggataccg cccttctccg
     1981 gatttttcag caaggaagag atcctgttgg cggcctggcg agcgaataaa ttcgtttacg
     2041 cgatggggtt gtttacgtcg gggctgacag ccttttatat gttcagactg tattttgcgg
     2101 tgttctggca ccggcctgta acagacggtc atcgacaccc tatgcccttt ttcgaatcga
     2161 tcccactggt gatcctcgcg ttgggggcgg cattcgcagg cttcattccc ttctcgagat
     2221 tcatactccc gggcgcgccc gagcgcgcgg ggggcggcat cggcgtcttt tcgatcgcac
     2281 cggtgggaat tgcactcgtg ggtatcggga tcgcagcctg gctttaccgg aaggaaagcg
     2341 accgtccgca aagggtcgcc acttcaatgc gcgggctcta tacgttcgca tatcataaat
     2401 tctatatcga cgaggtctat ttattcgtga cgaaaaagat catcttcaac ctggtgggcc
     2461 ggccgtcggc atggatcgac cggaatattg tggatgggct gatgaatggc atggcggcga
     2521 cgacggcgag gatctcggcg gggataaagg gggtacaatc ggggaaggtt caggattata
     2581 cctggttctt ttttatcggt atagccggat tggcagttat attcatttat atatacacat
     2641 gaacctttca ttactcatca ttgcgccgtt ggtgacggcg gtggcggtac tgttttgcag
     2701 agggcttagc gggatacgtt gggtatcgct ggccggcgtg accgtacaac tggcattgtc
     2761 gctggtgttg gccgtcctgt atgaaaagga acgcggcgcc ggagaatccg cccactggct
     2821 atttcagtac tggcacgcct ggttcatccc gttgaacatc gcctatcatg tcggtgtgga
     2881 tgggttgtcg gtggccatga tcctgctcac ggcattcgtg atgctggcgg ccgtactggt
     2941 ctcctggcgg atagacagga tgggaaagga attctttttc ctgctgctgg tcctcagcgc
     3001 aggggcctat ggttttttta tctcgctgga tatttttaca cttttctttt tcctggaaat
     3061 ttcggtaata ccaaaatttt tattgatcgg gatctggggt agcggacgaa aagaatattc
     3121 ggcgatgaag ctggccttga tgcttatggg cggatcggcc ctggtgctgg taggattgtt
     3181 ggggatatat accagcggag cgtatacttt cgacttgccg gcactcggcg gaaatctttc
     3241 attgcacgca cagcggctgt tcttcccctt cctgttcgtc gggttcggca ttttcacggc
     3301 gttgttccca ttccatacct gggtgcccga cggccattcc tccgcgccca cggccggatc
     3361 gatgttcctt gccggcgtca gcatgaagct gggaggttat ggctgtttgc ggatggcgct
     3421 gctgatgcct gcggtcgcca gggaatattc tccgtatatc atcgtcctgt cggtgatcgc
     3481 gatcgtatat ggtgcgttcg ctacgatgat gcagaacgac cttaaatata tcaatgccta
     3541 ttcctccgtc agccactgcg ggtttatcct gctgggcatc ggtatgctga cggaaacggc
     3601 cgtcaggggc gcggtcttgc agatgatctc gcatggattg atgacggctc ttttcttcgc
     3661 caccatcggg atgatctacg atcggaccca tacgcggctg gtcgcccggc tcggcggtct
     3721 gctgaaggtg atgccgttca tatctaccgt gttcgtgatc gcgggtctat gctccctcgg
     3781 gctgccgggg ctcagcggct tcgtagccga agtcaccgta ttcatgggat cgtggcaaca
     3841 cccgggacaa atgtatcatc tggcgacgat actggcctgc gccagcatcg tcattacggc
     3901 agtatatatc ctccgggcaa cgggggcgac ggtcataggt ccggtagggg actcgcaata
     3961 ttattctctg aaagatgcaa catggaccga acggctggcg gggatactat tggtgacggg
     4021 catcatcgcc attggcgtag cgccattctg gctgaacgaa cttatcgcta aatgaactaa
     4081 tgcttatgtg gacggacatt cttttttcat tgcgacagga gctggtggtc accgccatcc
     4141 ttttcctgct gctgttcatc aaggtcggcg cgagtgaata tcggacagaa acgctgctat
     4201 ggctgatcaa tattttgctg gcggtgaatt ttattttggg ttttttaccc ggtggaagct
     4261 cggtcctgtt cagcggcatg ttccatacca acgggatgat cgtgctggaa aagaacatcc
     4321 tcaacctggg caccctgatc gtatccttac tggcctatga ctggctaaag gaccaccggc
     4381 atgtcgccga gttctatatg ttgttattat ccacgctgct gggatttttc ttcatgatct
     4441 cctcgggaca tctgctgcta ttctatctgg gtctggaact ttccaccatt ccgttgacgg
     4501 cgctgtcaag tttcgatctt gaaaaaaggc aatcctccga ggccgctatg aaattcatta
     4561 tctcttcggc attctcatcg gccttattgt tgttcgggat ctcgctcctg tacggagcga
     4621 cggggaccct gagtttcgat acgatcccgc ggcacctgga cgggagtgcc ttccaactgt
     4681 ttgcctttat cctcattctg tcggggtttg cctttaagat atcggtggtc cccttccatt
     4741 tgtggactgc cgacgtgtat gaagggtcac cggtccctgt tacggcttac ttatccgtca
     4801 tctccaaggg aggcgtgttc ttcatattcg tacagacgct ttataccgtt tttgaaccga
     4861 tgggcgacaa ctggtaccgg atcgtggtgc tcctatccgt agtgaccatg ctgacaggta
     4921 acctgttcgc tttgcggcaa aacaatttca aacggctgct ggctttttct tcgatagccc
     4981 aatccgggtt tatcctggcg ggcatttcag gcagctcccg ttcgggggat gcctccgtca
     5041 tctacttcat cctcatttat gttttttcca atttaggtgc attcggagtc gtatcgctgg
     5101 tgagcgcggc gacgggaaag gaaaatatcg atgactataa ggggttttat gccaccaacc
     5161 cggtcctttc ctgggtgatg gctatctcgt tgttttcact ggccggcata ccgccgaccg
     5221 ccgggttttt cggaaagttc tttttgctga tggccggcgc ggggaaacac aatactatct
     5281 ttatcctcat tgcggcgctg aatatggtca tctcgttgta ttattatctc cgcgtgataa
     5341 aggttatctt tatggacagt cagccgcagc ctatccggaa actcgagata cccttgttgc
     5401 cgaatatcgc ttttatcctc tgtgtgacgg gtgtcctgct gacaggggtc atgggttggg
     5461 tgtatgatta tatttattca tggagcatag ggacttagta tggctatcaa tcaaaatcac
     5521 acttttgaag aactgaatgg cgtcaaatgc ggcatcgtcg agaagactgt gacgccggcg
     5581 cgggtagagt ttttgcgagg gatattggaa tataatcatt acaccgtcgt cgtggctccc
     5641 agcccgccgc caaagactgc tcctgcgact gcgcctgcgg aggctgcggc gtcttcgcct
     5701 gccggggccg aacctcctac ggcacccggg acgttcacgg tcggcgttac ggacgttaca
     5761 ttcaaccccg tgaatgccat attcgggcga ctgcttcgga cgccggaagg acatgtagta
     5821 acacttgctt attggcaaca gaaagatgct gcatccaatg acaccatacc ttattttacc
     5881 acagaatgaa attatctatt gtcgatagtc cgggatcgat ggccgctgat gtggccgaca
     5941 gggtgaaaga atgtttgcga ttcacatctc ggccgctgtt ttgccccgca tccggggaca
     6001 cgccggcggc attgtatgcc gagctgctgc ggagggctat ggatcatacc atgaatatgc
     6061 gggagtggtc ctttgtcgga ctcgatgaat gggtaggcat gaacggcgaa gatgcggggt
     6121 cctgtcgcta ttacatcgat aggggtcttt tccatccgct gaatgtgcct gctgacagga
     6181 tctgcttttt cgatgggcgc gcgcctgatc tgcaaaagga atgcgccgat gtcgaacgct
     6241 ttataggccg atggggtcgg atcgatgtcg cgatcctggg gatcggaccg aacggtcatg
     6301 tggcgatgaa tgaacccggc tccgacccag ccggacgcag tcaagtgtcc gagctccatc
     6361 cggccacgcg ggagacgggt cagaaatatt tcagttgtca ccagacgctt gagcgaggga
     6421 tcacccttgg gctgggcacg ctgatggagg cgcgtcatct gttcctgctt gccattggag
     6481 aaaagaaggc ggagattatc cggaaggcgc tggaaggccc gatcactaca gagctaccgg
     6541 cgagtttgct gcgcaatcat ccgggtttgg aggtatggct ggatgccggg gcggcgggtc
     6601 gctgagtgta gcagttatgc aaaaagataa acccctgtag aaacaggggt cgtaaccaaa
     6661 actaactgct tatgagaaaa ttgactgctt tatgttatac aaagatacga cggtgtgaag
     6721 ggcgacgctg ctttttagtg ttaaaatccc aataaagaaa cgttaaaagg acctgctccg
     6781 tacggatgcg ccatgaacgc ggccggaaga gacggttggg ttgctatttc aatgtatctg
     6841 aatgggttgt gaccttccaa tattcatccc gccagtcatc gggcaaatgg ggattttcat
     6901 ttatgtgtcg cccgatggat tccagctgtg cttcggaaag ttgtttttcc agatagggaa
     6961 acaggtcgcg ctcttcaaaa cgaatgtgat tgtcgacggc atccgccaat accggcaaca
     7021 ggcgcaggat accctctgag ggtcctgtta atatagagtc gaccaggact cctattcgct
     7081 gatgctcatc ctttgcccgg gcgatgccgg gatcatcggg caggatgaag aggaaggctt
     7141 cttcttcgct gaaatgaggt agcagatgat gctgccagaa atagtgtata taggccagta
     7201 tgcgcccggg ctcggttctg tgtttcaacc cctgtcttag tttccaacag aaaagtaatc
     7261 cggcatggtg atcatgcgac agggatacga tgttcttatt tcgccggatc ggttgcgttt
     7321 tcatatatct ttttttatag aagcattaag ccataaatga tactgagcac aagattaacg
     7381 ccgctgctca atacaaaaat cgctaataac catcgcatgg gggcttgcgc aaatgccagg
     7441 ctgtccgcga ggaaggccat gaggattccg aacgtaaaga tcagcgggga ggctccggcc
     7501 tcaccgagga tcaggatcat agcgccggcg aaggtcacct gtatgaggat ccctgccgcg
     7561 gcgataccga ggcggttttg ttcccaaatc ggttcccggg tccggaccca gttggtcagc
     7621 gactggataa ggcagctctt cttttcaatt aacgaggcgg ggagatggat ggggaagacg
     7681 gttgctttca tggcgaagaa ttttgtagca caaagttacc ggaccaaaaa agcaggaagt
     7741 atgatctaag tcatagagga ggctgatggt tgctaaggca cacgatctta ccgcgttggg
     7801 tggcgatcct gcccttcttc tctattcttt tcagcgtctt gatgaccgtc tcgacacgaa
     7861 gtccggtcat atcggcaagt tgttgtttgg tgatgtcgag gacattgttt tccgggttta
     7921 tccgcgccat gaggtaatgg atcagggtta gcaatcgatg ttcgccctct tcgttggcga
     7981 tctcctccag catcatcgtt ttgtattgca attgccg
//
LOCUS       DASYSV010000520        11252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2286716, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000520 DASYSV010000000
VERSION     DASYSV010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2286716"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VGQ51_16155"
     CDS             complement(<1..167)
                     /locus_tag="VGQ51_16155"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEV9038169.1"
                     /translation="MIWQREPAPDGGGKANIMARIAFTMKLHPGQTEEYRRRHDAIWP
                     ELSDLLHRTSIR"
     gene            complement(164..2284)
                     /locus_tag="VGQ51_16160"
     CDS             complement(164..2284)
                     /locus_tag="VGQ51_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HEV9038170.1"
                     /translation="MSVITTKFKHVSYLWDEKKAAEMAGDEVALLIYRSNLLGADLRL
                     TNYGGGNTSCKAIANDPLTGHETEIMWVKGSGGDLGTLKRSGLAALFVDRLLALKNVY
                     RGIKYEDEMVGLFNHCIYDLDSKAPSIDTPLHGFLPFAHIDHLHPDAAIAIAAAKDGR
                     KITQELFGGKIGWVEWQRPGFDLGLKLKKCLDDNPGIRGIMLGSHGLFTWGDTAYESY
                     INTLEVIERCAQYLEDHIGKKGPVFGGAKIQALPREQRLDKAAALAPILRGFCSSHTK
                     MIGHFTDDDRVLEFIDSKDLDRLAPMGTSCPDHFLRTKISPLVLDLAPAQDIGDVAQL
                     KQKLSPQFESYRQLYKQYYETCKHPNSPAMRDPNPVVILYPGVGMFTFSKDKQTARVA
                     AEFYINAINVMKGAEAISSYTALPRQEAFNIEYWLLEEAKLQRMPKPRALSGRIALVT
                     GSAGGIGKAIAKKFADEGACVIINDNDESRLDAARKEFQKQYGKDVFIGEVLDVTSAE
                     AIRKVYRAGALAFGGVDIIVNCAGLSISKPIQDHTEKDWDILYDVLVKGQFLVTQAGV
                     EVLRKQSIGGDVLNIVSKNGLVSGPNNAAYGSAKAAQLHLSRLNAAELGPDKIRVNVV
                     NPDAVISDSKIWEGAWAEGRAKAYGVSVQELPAYYAKRTLLNEIILPEDIANACFVFV
                     GGLMNKSTGNVLNVDGGVAMAFVR"
     gene            complement(2299..3378)
                     /gene="rhaT"
                     /locus_tag="VGQ51_16165"
     CDS             complement(2299..3378)
                     /gene="rhaT"
                     /locus_tag="VGQ51_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEV9038171.1"
                     /translation="MQIILGIFFHFIGGAASGSFYIPYKKVKGWAWESYWIIGGLFSW
                     LIVPPLAAWLTIPHFSDIIAQAGASTFWWTYFWGVLWGVGGLMYGLGMRYLGMSLGNS
                     VLLGFTSAFGALVPSIYYNFVHTPGKTTFHELLTTHWGRIVLIGVGLCLLGIFICGRA
                     GMLKERELPEEKKKESIQEFNLVKGLIVCIISGILSACFNYGIEAGSPMAEAANTFWK
                     QSHPGVTTEFLYQNNVTYIVLLWGGLTTNLVWCMLLNARNRTFGDYTNERAPLARNYL
                     FSALAGTVWFLQFFFYGMGESRLGNGASSWILHMAFIILVANLWGFALKEWKGVSPKT
                     KTTILMGILFILVAVGLVGYGNSLK"
     gene            complement(4098..4532)
                     /locus_tag="VGQ51_16170"
     CDS             complement(4098..4532)
                     /locus_tag="VGQ51_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV9038172.1"
                     /translation="MRTMLFSALMLSIFSLPVRAQSKDEQSVATAVESLRKALIDPDK
                     ATLDALVLDELTYGHSSGVIQDKAAFEEALLNKSSDFVTIDLSQQTIRVVGNTAWVRH
                     ILSATTNDGGKPGSTHLSVLLVWVKQKGQWRLLARQAVKAQA"
     gene            complement(4546..5862)
                     /locus_tag="VGQ51_16175"
     CDS             complement(4546..5862)
                     /locus_tag="VGQ51_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEV9038173.1"
                     /translation="MLAYPVRIALIAALGGFLFGFETVVISGAEQTLEQFWKLHGLMQ
                     GFTVSSSIFGTVLGALVAGIPAKRYGRKRVLQTIAVMFVVAALGCAFVSAWGLFILFR
                     IIGGLAVGASSVIAPMYISEISPATMRGRLAGSFQVNIVAGIVVAYLTNYLFLQLQLT
                     DSWRWMLGVMVIPAVIFWILLLAIPESPRWLILNGDEQAALPILKRLGEQNLAATITA
                     IRESVKEHQESLFQKRYSKPIVYAMLLAMFNQLSGINGILYYAPRFFTMAGFDKTQAF
                     SQPIYIGVANLLFTLLAMTVIDKFGRKTLLIIGSLGMIVFLGLTAWAFKTHTAGSNLL
                     FYLIGFIAFFAFSQGAVIWVFISEIFPNSVRSQGGSLGSSTHWGMAWIITTIVPGILE
                     GSANGAYYSFAFFAFMMVLQFVFIWRVMPETKGRTLEEIQKDLGIS"
     gene            5915..6949
                     /locus_tag="VGQ51_16180"
     CDS             5915..6949
                     /locus_tag="VGQ51_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV9038174.1"
                     /translation="MKYEAITIKDIAKALGLSTSTVSRALRGSYEISAETRKLVLEYA
                     EKLNYRPNPIALSLKEKRSRSIGIVVCEIANNFFSQVINGIESVAYKKGYYVIISQSH
                     ESFEREVANTQYLASRSVDGLLVSLSTETTDLSHFTMLHDKGLPIVYFDRIPEEMPTH
                     KVIANNFNGAYQATEHLIHSGFKRIAHLTISPHLSITKERLAGYKAALADHRLPFDEA
                     MVKYCKYGGLVEEETEEAVKGLLSLSKRPDAIVAASDKLSTGCLAVLARKKIRVPDEM
                     GIVGFTNSLVTDIFHPSLTSIRQPAFEMGQVAMEMLIQLIESKHPVSKFETRVLNTEL
                     TVRSSSVKAG"
     gene            complement(6976..8265)
                     /locus_tag="VGQ51_16185"
     CDS             complement(6976..8265)
                     /locus_tag="VGQ51_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9038175.1"
                     /translation="MSKSPVGRYRWSVCALLFFATTINYMDRQVIGLVEPTLKHDFGW
                     SDTTFGTINGIFQFFYASGLLLFGAIIDRVGTKIGYAISIIGWSLSAMGHGLAKATTG
                     FTVARSALGLSEAGNFPSAIKAVAEWFPKRERALATGIFNSGANIGQVVAPLMVPPIL
                     AIYGWQAAFIATGALGLIWLVFWWLFYEIPSRKKNLSPEEFKYIHSDDEDTGSATGSR
                     VSWGKLLSVRQTWAFVFGKFLTDPIWWFYLFWLNAYFHDVFHTSDKKITWPLVVIYSC
                     TTIGSIGGGYLSGYLIRKGWPIYKARKTVMFIFALCVVPVVSAGYLDNMWLITAVIAV
                     AASAHQAWSANIFTTASDMFPKKAVSSITGIGGMAGSIGGIIFQPAVGLLLDYFSKHN
                     NKTLGYTIIFLICGSAYLLAWVIMHLFAPKQTRVQLD"
     gene            complement(8298..9128)
                     /gene="kduI"
                     /locus_tag="VGQ51_16190"
     CDS             complement(8298..9128)
                     /gene="kduI"
                     /locus_tag="VGQ51_16190"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEV9038176.1"
                     /translation="MEIRFEHSPKEVKGMSTEELRSAFLTEPLMQPDTIRLVYTHYDR
                     VIIGGACPVRQKLTLEAHPELRADFFLQRREIGIINVGGNGTVYADDKSYPVGRLECL
                     YAGKGVKKIVFGSDDPANPARFYCLSSPAHAEHPVQFLPKEKAMPVKTGSAETSNART
                     IYKYIHVDGIRSCQLVMGLTILETGSVWNTMPAHTHTRRMEAYFYFDVPEGQRVFHFM
                     GEPQETRHLLVADGQAIVSPPWSIHSGCGTSNYAFIWGMAGENYTYTDMDAAPIPDLR
                     "
     gene            complement(9162..9926)
                     /locus_tag="VGQ51_16195"
     CDS             complement(9162..9926)
                     /locus_tag="VGQ51_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV9038177.1"
                     /translation="MSNLFNLEGKSALVTGCNRGIGRSMAVGLAKAGADIIGVSSSMP
                     LKDSDVEKEICALGRRFSAYAADLTDRGAIYELLRGVKAEHDRIDILVNNAGMILRAP
                     AAQHSDEYWDKVLSINLDAAFILARELGRDMLVRRSGKIIFTCSLLSFQGGINVPGYA
                     ASKGALSSLVKALANEWAGQGVNVNGIAPGYISTDNTEALRNDPIRSKAILDRIPAGR
                     WGEPEDFQGPAVFLASAASDYVHGTILTVDGGWMGR"
     gene            10110..11144
                     /locus_tag="VGQ51_16200"
     CDS             10110..11144
                     /locus_tag="VGQ51_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV9038178.1"
                     /translation="MKFEAITIKDIAKALNLSVSTVSKALRGSHEISQETKKNVLEYA
                     RANNYKPNPIAQSLKRGRSKSIGVIVCNIDNNFFSQAINGIESIARQKDYNVIITQSR
                     ESYEREIANSEHLSSRSVDGLIISLSAETRCVDHLIKLNEKGLPMVFFDRITDEIETH
                     KVIANNHLGAYEATRHLLQQGFRRIAHITSSRSLSITQERFEGYKAALQDAGLQPDPA
                     LIKFVQHGGMIQEETSQALSQLLEMENGPDAIFTASDRLSTTTLSLLRKMNIEVPKQI
                     ALVGFTNSISADIFHPSLTAVVQPALEMGQLATELLIQLIESKRPVVEFQKKVLETEL
                     KIRDSSLRPR"
BASE COUNT         2695 a         3129 c         2997 g         2431 t
ORIGIN      
        1 cgaatactgg ttcggtgcag gaggtccgac agttcgggcc agatcgcatc gtgccggcgg
       61 cggtattctt ccgtttgccc aggatgcagc ttcatagtaa aagcaattcg tgccataata
      121 ttcgcttttc cgccgccatc cggggccggc tcccgttgcc agatcatctg acaaaagcca
      181 tcgctactcc gccgtcgaca ttcaatacat tccccgtgga tttattcatc aacccgccga
      241 cgaacacgaa acatgcattt gcgatatctt cgggcaggat gatctcgttg agcagcgtcc
      301 ttttggcata ataggcgggg agctcctgga ccgagacccc ataagccttg gcgcgtcctt
      361 ccgcccaggc gccttcccat atcttgctgt cggagatgac ggcgtccgga ttgacgacgt
      421 tgacgcgtat cttgtcgggt cccagctcgg ccgcattaag ccggctcaga tgcagctgtg
      481 cagccttggc ggatccatag gcggcattgt tgggccctga tacaagaccg ttcttgctga
      541 cgatattaag gacgtctcct ccgatacttt gtttccgcaa tacttccaca cctgcctgtg
      601 taaccaggaa ctgacccttg acgagcacgt catagaggat gtcccagtct ttttcggtgt
      661 ggtcctgtat cggcttggaa atggagaggc cggcgcagtt gacgatgata tccacgccgc
      721 cgaatgcgag tgcgcccgca cggtagactt tccggatagc ctcggcgctg gtcacatcga
      781 ggacttcccc gatgaacacg tctttcccat attgtttctg gaattccttg cgcgccgcat
      841 ccagccggga ttcgtcgttg tcgttgatga tcacgcaggc gccttcatcc gcgaattttt
      901 tggcgatggc tttaccgatg ccgcccgcgc tgccggtcac cagtgcgata cggcccgaca
      961 gggctctggg ttttggcatc cgctggagct tggcctcttc gagcagccag tattcgatat
     1021 tgaacgcttc ctgccggggt aaggcggtat aggaagagat ggcttcggcg cccttcatga
     1081 cattgatggc attgatatag aattccgccg ccacccgcgc ggtctgcttg tccttcgaaa
     1141 aggtgaacat accgacgccg ggatagagga tgacgaccgg attcgggtcg cgcatggccg
     1201 ggctgttggg atgtttgcag gtttcataat attgtttgta cagctgcctg tagctctcga
     1261 attgagggga cagcttttgt ttgagctgag cgacatcgcc gatatcctgt gcaggtgcga
     1321 ggtccagcac cagcgggctg atcttggtgc ggaggaagtg gtcgggacag ctggtcccca
     1381 tcggtgcgag acggtccagg tctttggaat cgatgaactc cagaacgcgg tcgtcatcgg
     1441 tgaaatgacc gatcatcttc gtatgcgagg agcagaatcc gcgcaggatg ggcgccaatg
     1501 cagccgcttt atcgagccgt tgctccctgg gcagggcctg tatctttgcg ccgccgaaga
     1561 cggggccttt tttcccgata tggtcttcca ggtattgcgc gcaacgttcg atcacttcca
     1621 gcgtattgat ataactttcg tatgccgtat cgccccacgt gaacagaccg tgcgagccca
     1681 gcatgatccc gcggatgccc gggttgtcat cgagacattt ttttagcttt aagcccagat
     1741 cgaaaccggg acgctgccat tccacccagc cgatcttgcc gccgaaaagt tcctgcgtga
     1801 tcttcctgcc gtctttggca gcggcgatgg cgatggccgc atcgggatga aggtgatcga
     1861 tatgtgcgaa agggaggaag ccgtgcaacg gggtatcgat ggatggcgct ttggaatcga
     1921 gatcatagat gcaatgattg aaaagaccga ccatctcgtc ttcatatttg attccgcgat
     1981 agacgttctt cagcgcgagc agcctatcga caaagagagc cgccagaccg ctgcgtttca
     2041 aggtccccag gtctccgccc gatcccttca cccacatgat ctccgtctcg tgaccggtca
     2101 acgggtcgtt cgcgatggct ttgcaggaag tgttcccgcc gccgtaattg gtcaaacgca
     2161 gatcggcccc cagcaggttc gagcgataga tgagcaaggc aacttcatcc cccgccatct
     2221 ctgctgcttt cttttcatcc cataggtagc tgacatgctt gaatttcgtg gttataacag
     2281 acatatgctt atacttttct atttcaatga attcccgtag ccgacgagtc ccactgccac
     2341 caggatgaac aggatgccca tcaggatcgt cgtttttgtt ttcgggctta ctcctttcca
     2401 ttccttcagt gcgaaacccc atagattcgc tacgaggatg atgaacgcca tgtgcaggat
     2461 ccacgagctg gcgccattgc cgagacggct ttcccccatg ccataaaaga aaaactgcag
     2521 aaaccacaca gtgcccgcca gcgccgaaaa cagatagttg cgtgccagcg gtgcgcgttc
     2581 gttggtatag tcgccgaagg tcctgttccg ggcattgagg agcatacacc acacgagatt
     2641 tgtcgtgagc cctccccata acaggacgat ataggtgacg ttattctggt acaggaattc
     2701 cgtcgtgacc cccggatgcg attgtttcca gaatgtgttg gcggcttcgg ccatcggcga
     2761 acccgcctcg atgccatagt tgaaacaggc gctgagtatc ccggagatga tacaaacgat
     2821 caggcctttg acaaggttga actcctgtat gctctctttc tttttttctt cgggcagctc
     2881 tctttccttc agcatccccg ccctcccgca aatgaagata cccagcagac aaagccccac
     2941 gcctataagc acgatgcgac cccaatgggt ggtcagcaat tcgtggaagg tcgttttgcc
     3001 gggggtgtgt acaaaattat aatagatgga ggggaccagc gcgccgaatg cggaggtgaa
     3061 acccaataat accgagttgc cgagcgacat gccgagatag cgcataccca ggccgtacat
     3121 cagcccgccg acgccccaga gtactcccca gaaatatgtc caccagaagg tggaggctcc
     3181 cgcctgtgcg atgatgtccg aaaaatgcgg gatggtcagc catgctgcca gcggcgggac
     3241 gatgagccac gagaacagac cgccgatgat ccaatagctt tcccacgccc aacccttcac
     3301 ttttttatag gggatgtaaa agctgcccga agcggcgcct ccgataaaat ggaaaaaaat
     3361 gccgaggatt atttgcatga ataccagtga ttttcgatat ggctgtacaa acgatggtca
     3421 aatgtaaaac ggaatgccgg gagaaccatc atgtactgtc atggatgggg ctttggctaa
     3481 atataactga cctgaagacg aatatcgttc agagtcgcta tgccgccatg cggctcgcca
     3541 ataaagagca gttactgctt tatttcaaaa ccgggaagat gctgtcggaa aaaattgcta
     3601 tacagaaatg gggaacgaaa gtggtcgaac aaatagctac cgatctgcaa aaggaattgc
     3661 cgggattgag gggattctct tatcggaatc taatgaatat gcggagattt tattctgagt
     3721 atcaagacgt tatgattctg cagacagagt ctgcagaatt ggagagattc tggttgatca
     3781 ccacatcggt gcaaatttgt ataagtatca gggtaagtta cccaataact tcaacaaaac
     3841 attacccgac gagctaaaat cctccgccct cgaagtcttc cgggatgaat atctaatgga
     3901 tttcatttcc gaatccgaca ccgaagacga acgccaattc gaatccaaag tcctggcgga
     3961 catcaaaaat ttcgtccttg tcatgggcaa gggcttttgc tttatcggca atcagtacag
     4021 ggtcgacgtg cacgaggaag aattcttcat tgtctacccc cggcgaagga actggcgcgt
     4081 ttgttatagg cacggcgtca cgcctgcgcc ttcaccgcct gccgtgcgag taagcgccat
     4141 tgccctttct gcttcaccca caccagcagt actgagaggt gggtggaacc cggtttccct
     4201 ccgtcattgg tagtggcact caggatatgc ctgacccagg ccgtattgcc gacgaccctg
     4261 atggtctgct gactgagatc gatggtgacg aaatcggatg acttgttgag cagtgcctct
     4321 tcgaatgccg ccttgtcctg gatgacgccg ctggaatgac cataggtcag ctcatcgagg
     4381 accagcgcat ccagcgttgc cttgtcgggg tcgatcaatg ccttgcgcaa cgattcgacg
     4441 gcggtggcga cggattgctc gtctttggat tgcgcacgga ccggcaagga aaaaatggac
     4501 agcatcagcg cggaaaacag catagttcgc atatgttggt aatttttaag atattcccag
     4561 atctttctgg atctcctcca gcgtacgccc tttcgtctcg ggcatcactc gccagataaa
     4621 gacaaattgc aataccatca taaaagcaaa aaaggcaaac gagtaatacg ccccgtttgc
     4681 gctaccctcc agtatgcccg gaacgatggt ggtgatgatc caggccatac cccagtgtgt
     4741 agaactgccc agtgagccgc cctgcgaccg gacggaattg ggaaagatct ctgaaatgaa
     4801 aacccagatg acggcgccct gggaaaaggc gaaaaaggcg ataaacccga tcaggtaaaa
     4861 taaaagattg ctgcccgccg tatgtgtctt gaaggcccag gcagtgagac cgagaaaaac
     4921 gatcatgccg agcgaaccga ttatcagtaa cgtcttacga ccgaacttgt cgatgaccgt
     4981 catcgccagc agcgtgaaca aaagattcgc taccccgatg tatatcggct ggctgaaagc
     5041 ctgggtcttg tcgaaacccg ccatcgtgaa aaaacgcggc gcataataca agatgccgtt
     5101 gatcccggat aactggttga acatggccag caacatggca taaacgatcg gtttcgaata
     5161 ccgcttttgg aacagcgatt cctggtgttc ctttaccgac tcgcggatcg cggtgatcgt
     5221 cgccgccagg ttttgctctc ccagcctttt caatatcggc aatgcggcct gttcatcccc
     5281 attgaggatc agccagcggg ggctctcggg aatggcgagc agcagtatcc aaaagatgac
     5341 cgcggggatc accataaccc ccagcatcca tcgccatgaa tccgttagct gcagctgcag
     5401 aaataaatag ttggtgagat aggcgacgac gatgcccgcc acgatgttca cctggaaaga
     5461 accggctaac cggccgcgca tagtagccgg cgatatttcg gagatataca taggtgcgat
     5521 caccgatgat gcacctaccg caaggccccc gatgatccga aagagaataa ataaccccca
     5581 ggcggacacg aaggcgcatc ccagggcagc caccacgaac ataacggcaa tcgtctgcaa
     5641 tactctctta cgaccatatc tttttgcggg tatacccgct accagcgcac cgaggaccgt
     5701 gccgaaaata ctggaggaga ccgtgaatcc ctgcatcaga ccatgcagct tccaaaactg
     5761 ttcaagcgtc tgctccgccc ccgatatgac cactgtctcg aatccgaaca ggaagccgcc
     5821 gagggcagcg atcagcgcaa tacgaacggg ataggccagc atggcagaca atttattgtg
     5881 tcaccgaaaa taatactaat tttaatcaat acggatgaaa tacgaagcca tcaccataaa
     5941 agacatcgcc aaagccctgg gcctttcgac gtctacagta tccagggccc tcagagggag
     6001 ctatgagatc agcgcggaaa ccaggaaact cgttctcgaa tatgcggaga agctcaatta
     6061 ccggcccaac cccatcgcct taagcctgaa agagaaacga agccgatcga tcggtatcgt
     6121 tgtttgcgag atcgccaaca atttcttttc gcaggtgatc aatggtatcg aatcggtggc
     6181 ctataaaaaa ggatactacg tcatcatctc gcaaagccat gagtcctttg aacgcgaggt
     6241 cgccaatacg caatacctag cttcacgctc ggtggacggg ctgctggtct cgctttccac
     6301 cgagacgacg gatctctcgc atttcacgat gttacatgat aagggtcttc caatagttta
     6361 tttcgatcgg ataccggagg agatgcccac gcataaggtg atcgcgaaca acttcaacgg
     6421 cgcctatcag gccaccgaac acctgatcca cagcggcttc aaacgaatcg cgcatctcac
     6481 catttcgcct catctgtcga taacgaagga aagactggcc ggctataagg ccgcccttgc
     6541 agatcatcgt ctccctttcg acgaagccat ggtgaaatac tgcaaatacg gcggactggt
     6601 cgaagaagaa accgaagagg ccgtcaaagg cctgctgtcg ctatcgaaaa gacccgatgc
     6661 catcgtcgcg gccagcgaca aactctccac cgggtgtctg gccgtactgg ccagaaaaaa
     6721 gatacgggtc cccgatgaga tgggtatcgt aggtttcacc aattcactgg tgacggatat
     6781 ctttcaccca tcgctgacct ctatccggca gcccgcattc gaaatgggtc aggtggcgat
     6841 ggagatgctg atccagctca tcgaaagcaa acatcccgtc tcgaaattcg aaacacgtgt
     6901 cctcaatacg gaactgaccg tccgctcgtc gagcgtcaag gccgggtagg ctgaggcgta
     6961 gggccgagtc aagccctaat ccaactgcac acgcgtctgc ttgggcgcaa acaaatgcat
     7021 gatgacccac gccaacagat atgccgaccc gcagatcagg aagatgatcg tatatcccag
     7081 cgtcttatta ttgtgtttgc tgaaataatc cagtagcagc cccacggcag gctggaagat
     7141 gatgccgccg atcgaacctg ccatgccgcc gatcccggta atcgagctaa ccgccttttt
     7201 ggggaacatg tcggacgcag tggtaaagat gttggcgctc catgcctggt gcgcagaagc
     7261 agcaacagcg atgacagccg tgatcagcca catattatcc aggtaacccg ccgagacgac
     7321 cggcacgaca caaagcgcga aaatgaacat tacggtcttg cgagccttgt aaatgggcca
     7381 gcctttgcgg atcaggtatc ccgagagata gccgccgccg atgctgccga tcgtcgtgca
     7441 ggaatagatg accaccagcg gccaggtgat ctttttgtca ctcgtatgga aaacgtcgtg
     7501 gaaataagcg ttcagccaga agaggtagaa ccaccagata ggatcggtga ggaacttgcc
     7561 gaatacgaag gcccaggtct gacgcacgga caggagcttt ccccaggaaa cccggcttcc
     7621 ggtagccgat ccggtgtctt catcgtcgct atgaatatac ttgaactctt cgggactcag
     7681 gttcttcttc cgcgatggga tctcatagaa cagccaccag aaaacgagcc agatcaaacc
     7741 gagcgcgccg gtcgctatga aagccgcctg ccagccgtaa atggcaagga tcggcggcac
     7801 catcaatggc gccactacct ggccgatgtt cgcacccgag ttgaatatac ccgtcgcgag
     7861 cgcccgctcc ctcttgggga accattccgc caccgccttg atcgctgaag gaaaattgcc
     7921 ggcttcacta agcccaagcg cggaccgtgc cacagtgaac ccggtcgtgg ccttcgccag
     7981 cccatgaccc attgccgaca ggctccagcc gatgatcgag atggcataac cgatcttggt
     8041 ccctaccctg tcgatgatcg cgccaaacag cagcagaccg gacgcataaa agaactgaaa
     8101 gataccatta atggtcccga acgtggtgtc actccatcca aaatcatgtt tcaatgtcgg
     8161 ctctaccaga ccgatgacct gccggtccat atagttaatg gtggttgcga aaaagagcag
     8221 tgcgcagact gaccagcggt accggccaac aggagattta gacatgggcg ttcaattaga
     8281 gaggccaatt taatcaatta tcgcaaatcc gggatgggcg ccgcatccat gtcggtatag
     8341 gtatagttct cacccgccat gccccagata aaggcataat tgctggtgcc gcatcccgaa
     8401 tggatcgacc atggcgggga aacgatcgcc tggccgtccg ccacgagcag gtgccgtgtc
     8461 tcctgcggct cacccataaa atgaaaaacg cgctgacctt cggggacgtc gaaataaaaa
     8521 taggcttcca tcctacgggt atgggtatgc gcaggcatcg tgttccagac gcttcccgtc
     8581 tcgaggatgg tgagacccat caccagctgg cagctccgga taccgtcgac atgaatatac
     8641 ttgtaaatgg tacgtgcatt ggacgtctcc gcggaaccgg tcttcaccgg catcgctttt
     8701 tccttaggca ggaattgcac ggggtgctcc gcatgggccg gagaggacag acaatagaac
     8761 ctggctgggt tcgcggggtc gtcgcttccg aagacgatct tctttacccc tttcccggca
     8821 taaagacact ccaggcggcc cacggggtag gacttatcgt ccgcatacac cgtgccgtta
     8881 cctcctacgt tgatgatgcc gatctcgcgt ctttgtaaaa agaaatccgc gcgcagctcg
     8941 ggatgcgctt cgagggtcag cttctgccgc acagggcacg cacccccgat aatgacccgg
     9001 tcgtaatgcg tataaacgag acggatcgta tcgggctgca tcagtggttc cgttaggaag
     9061 gcgctccgca gctcttcggt gctcatgccc ttcacttcct tcgggctgtg ttcgaatcgt
     9121 atttccataa ccttaattta tcttcccatc cagccgccaa tctatcttcc catccagccg
     9181 ccatcgacgg tcaggatcgt gccatgcacg taatccgatg ccgcgcttgc cagaaaaacg
     9241 gccggtccct gaaaatcttc gggttcaccc caacgaccgg caggtatcct gtccaatatg
     9301 gccttgctgc gtatgggatc gttgcgcaaa gcctcggtat tgtcggtgct gatatagccg
     9361 ggcgcgatgc cgttgacatt gacaccctgt ccggcccact cattggccag ggccttgacc
     9421 aggctggaaa gcgccccctt gctggcagca taacccggga cattgatgcc accctgaaag
     9481 ctcagcaggc tgcaggtgaa tatgatcttg ccacttcggc ggaccagcat atccctgccc
     9541 agctcgcgag cgaggataaa ggccgcatcg agattgattg acagtacctt gtcccaatat
     9601 tcgtcggaat gctgtgccgc cggggcccgg aggatcatac ccgcattatt gaccaggata
     9661 tcgatccgat cgtgttcggc ttttaccccc cgcaacagct catatatggc gccgcgatcc
     9721 gtgagatcgg cagcataggc cgagaatcgc cgtcccagtg cgcatatctc cttttcaacg
     9781 tcgctgtcct tcaagggcat ggaggaggat acgccgatga tatcggctcc tgcctttgcc
     9841 aggccgaccg ccatgctgcg gccgataccc ctgttgcagc cggtgaccaa tgctgactta
     9901 ccctccagat taaacagatt gctcataaag aaattaccgg acagttgtta tatttgaccg
     9961 gatcaccctg aagtgggcct gcgggcagcc ggattgacgg tgcttagcgc cggccctccg
    10021 gtacatttgg caattgtaca ctgtaattca ataacatcgg gggaatatcc gatctttttt
    10081 tacgaaaacg tttgcggcga aatcccatca tgaagttcga ggcgatcacc ataaaagata
    10141 ttgcaaaggc gctgaatctg tcggtctcca ccgtctccaa agcactgcgc ggcagccatg
    10201 agatcagcca ggagactaaa aaaaatgtcc tggaatatgc acgcgccaat aattacaagc
    10261 ctaaccccat cgcccaaagc ctgaaacgag gccgcagcaa atccatcggc gtcatcgtct
    10321 gcaatatcga caacaatttc ttttcgcagg cgatcaatgg catcgaatcc atcgcgcggc
    10381 aaaaggatta caatgtcatc attacccaaa gccgggaatc ctatgagcgc gagatcgcaa
    10441 atagcgaaca tctttcttca cgttcggtgg acggattgat catctcgctt tccgcggaga
    10501 cgcgctgtgt cgatcacctc atcaaattaa acgaaaaagg actgccgatg gtttttttcg
    10561 atcggatcac cgacgagatc gagacccata aggtcatcgc caacaaccac ctcggcgcct
    10621 acgaggcgac ccgtcatctg ctgcaacagg gattccggcg gatcgcccat atcaccagca
    10681 gccgcagcct gtccattacg caggaaagat tcgagggcta taaggccgcc ttgcaggatg
    10741 cagggttgca acccgatccc gcattgatca agttcgtcca acatggtggc atgatccagg
    10801 aagagacgtc gcaagccttg tcccagctgt tggagatgga gaacgggccg gacgccattt
    10861 ttaccgcaag cgaccggctg agcacgacca cgctgagcct tctccgtaag atgaacatcg
    10921 aggtcccgaa gcagatcgcg ctcgtgggct ttaccaattc catatccgca gatatttttc
    10981 atccctcact gacggcggtg gtacagccgg cgctcgaaat gggacagctg gcgaccgagc
    11041 tgctgatcca gctgatcgaa agcaagcggc ccgtggtgga gtttcagaaa aaagtattgg
    11101 agacggagct gaagatacgg gattcgagcc tgcggccccg gtaaagcatg aggccgcgag
    11161 gggtggccgg tgcgagaggg tggccggtgc gagagggtgg ccggcgcgag agggtggccg
    11221 gtgcgagagg tggtcggcgc gaggggtggt cg
//
LOCUS       DASYSV010000521         4675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1133223, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000521 DASYSV010000000
VERSION     DASYSV010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133223"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..624)
                     /locus_tag="VGQ51_16205"
     CDS             complement(88..624)
                     /locus_tag="VGQ51_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038179.1"
                     /translation="MRTLMAILASMIVLGATAQTHLAIPAYPYAPSQQLWLTPSFYFN
                     PGFVPFDQSARKWQIKPYASVSAGYIFLNGGISYLSTPVGFTFARPLNNNIAAYTNLS
                     LAPTIFSMSSLYSAPAGLSPYSGAGYGLSTRIEGGLIYSNDARTFSISGGVSIERGSY
                     QLFQPAAPHSAKTSPIFK"
     gene            complement(660..2051)
                     /locus_tag="VGQ51_16210"
     CDS             complement(660..2051)
                     /locus_tag="VGQ51_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9038180.1"
                     /translation="MNRKSFLRNSGLAALALAASPSSKAHAHQTTSAPLNTTPLHFAG
                     PKVRLAILGVGLRGQSHLELMLRRDDVDIAAICDVDDYMLSRAKDMIAKSGKKAAQAY
                     TGSPYAWQDLLRNEHLDAVLIASPWEWHKPMIIGALGSGLKYVATEVILGLTVQDHWD
                     VVKSAEQHNANVMMLENVCYRRDVMAVLHMVRQGLFGELIHLQGGYQHNLQGIKFNSG
                     AHDGGVGCEFGEGAFSEARWRTNHSVHRNGDLYPTHGIGPLAHYIDINRGNLFTTLCS
                     FSTKTRGLHDYVVRHCGDNHPSAKVKFKLGDIVTTSINCANGETILLQHDTNLPRPYS
                     LGFRVQGTNGLWMDVANGIYVEGQSKNDAWDPASAWLEKYDHPLWKRWSKEAEGAGHG
                     GMDFFVDHAFIESVKRKTPTPQDVYDAAAWSVITPLSEQSIEMGNATVAFPDFTGGQW
                     MYRKPTFALGDEY"
     gene            2183..3319
                     /locus_tag="VGQ51_16215"
     CDS             2183..3319
                     /locus_tag="VGQ51_16215"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV9038181.1"
                     /translation="MIPDGNISESTGTTLSYTPQALLNAVGNSLQSPLFNEVFRIKGI
                     YKYGKGVNYNGVYYDILRDEQSDSNMTLVTPERMRTALRDGQLIEASAFLSRRLQTAT
                     SRIDLLLTLNELFSRKASMVSEEETRALALLRRKAAIGYRDADSFIRHRLFGQQPIRI
                     TILVGTTAIVDQDIKHQVKEAAVAYRMEYVRINLAQPAEIDRAMKRHNDTDILVIARG
                     GGENLRVFDLPELAETALEIRPIFITAIGHSTDEPLLQKVADKSFITPTALGQYLRDV
                     YDRAMDEFNDSKAKLIGDLTKQIDLNYQARLQDQQTRLQDLQTRLTESSRTNQEAIRH
                     SEERLQTFSRQLSKTRNFNQWLIALLVASILISLYLWVSVRHGG"
     gene            complement(3386..>4675)
                     /locus_tag="VGQ51_16220"
     CDS             complement(3386..>4675)
                     /locus_tag="VGQ51_16220"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9038182.1"
                     /translation="RWMATDLRFDLFDWKVWTGIIGVGLFTGIIAGSYPALFLSKFLP
                     AKVLKGEMTIGRKRAVFRRAMVTTQFWIAILFIISTIVFWQEIQYIKDRPIGYDQENL
                     VDVHASADLGGKYHLFTNEVSRLPGVRSISAGSENLVNFPGAVTGMNWPGKIPGHEIS
                     ILITTVGYNWVKTAGLSLAEGRDFDPAFGTDTSACLVNESTVTRLGLHEPVLGQKLGA
                     SPIIGVVKNFVTNNPSGIIAPMAIYLYKGAPGNGHFFVRIANNDHWRQTIAGIGSVVK
                     TLDPRHGFDYSFTKEEYQRRFEEFNNYGTLATIFGGLAIFISCLGLLGLAGFVAERRA
                     REMSIRKVFGASVRQVLLLLSADFLRPVFLAFVLAVPVAFWAMHAWLDNIIYHVLLSW
                     TVFAAGGLLALAIALATVGWQGIRTANENPAKKLRNE"
BASE COUNT         1084 a         1302 c         1289 g         1000 t
ORIGIN      
        1 gccaatggcg ggaggggtgt aagagatatc gtcattctca gcgccaagac gccgcccgcc
       61 aatatgccga tggggccggt acccgagtca tttaaaaatt ggtgaagtct tcgcggaatg
      121 gggtgcggca ggttgaaata actgataact ccccctttct atgctgacgc ctccggaaat
      181 ggaaaaggtc cttgcgtcat tggagtatat gagtccccct tctatccggg tacttaagcc
      241 atacccggcg ccggaatatg gcgacagccc tgccggagcc gagtaaagac tgctcatgct
      301 aaagatcgtg ggcgccaaag agagattggt ataagccgca atattattgt tcaacggtct
      361 ggcgaatgta aaccccaccg gcgtggacaa ataagatatc ccaccattta gaaagatata
      421 gcctgccgac acgcttgcat acggcttaat ctgccatttt cgggccgact ggtcgaatgg
      481 aacgaaacca ggattgaaat aaaaggaggg cgtcagccaa agctgctgcg agggagcata
      541 aggatatgcg gggatcgcca ggtgggtctg agccgtcgca ccaaggacga tcatcgatgc
      601 cagaatcgcc atcagggttc gcatggtgga agattgtact ataaaggtac ctattaaatt
      661 caatattcat cccccaacgc aaatgtcggt ttgcgataca tccactggcc gccggtaaaa
      721 tcggggaagg cgacggtagc attccccatt tcgatagact gctcgctcaa tggtgtgatc
      781 acgctccatg ccgccgcgtc atagacatcc tgcggggtag gcgttttccg cttgacggat
      841 tcgataaaag catggtcgac gaaaaagtcc attcctccat gccccgcacc ttccgcctcc
      901 ttgctccagc gtttccacag cggatgatcg tatttttcaa gccaggcgga cgcggggtcc
      961 catgcatcgt ttttcgactg tccttccaca tagatgccat tggctacatc catccagagt
     1021 ccgttggtac cctggacgcg gaagcccagc gaatatggcc gcgggagatt ggtatcgtgc
     1081 tgaagcagga tggtttctcc attggcgcaa ttgatagaag tcgttacgat atcgcccagc
     1141 ttgaatttca ccttcgcgct gggatggttg tccccgcaat gccggaccac gtaatcatga
     1201 agaccacggg tctttgtaga aaatgaacag agggtggtga agagattgcc gcgattgata
     1261 tcgatgtaat gcgccagcgg gccgataccg tgggtcggat aaagatcgcc gttgcgatgg
     1321 acggaatggt tggttcgcca gcgggcctcg gagaatgccc cttcaccgaa ctcgcagcct
     1381 acgcccccgt cgtgggcacc gctgttgaat tttattccct ggagattgtg ctggtagccg
     1441 ccctgcagat ggatcagttc cccgaagagt ccctgccgga ccatatgcaa cacggccatg
     1501 acatcgcgtc ggtaacagac gttttccagc atcatgacgt tggcgttgtg ctgttcggca
     1561 gacttgacga cgtcccaatg atcctgtacc gtcagaccga ggatgacctc ggtggcgaca
     1621 tatttcagac ccgacccgag cgctccgatg atcatcggtt tgtgccattc ccagggggag
     1681 gcgatgagga cagcgtcgag gtgttcgttc ctgagcaggt cttgccaggc ataggggctg
     1741 ccggtatagg cctgcgctgc ctttttcccc gatttggcga tcatgtcttt cgctcgtgac
     1801 agcatatagt cgtcgacatc acagatagcg gcgatgtcga cgtcatcgcg gcgaagcatc
     1861 agctcgagat gactttggcc gcggaggccg acgccgagga tggccaggcg aaccttcggg
     1921 ccggcgaaat gaaggggcgt ggtattcagg ggtgcggagg tcgtctgatg cgcatgggcc
     1981 tttgaggaag ggctggcggc aagggccagc gccgcgagac cgctattccg aaggaaagac
     2041 tttctgttca tatcgaagca acgatttttg tcaaacgtac tggttttcgg tcgtagttcc
     2101 ccggcgcaaa cgattgctcg aattaaaaag cgggaggaat gagcggaggc ttttgctaaa
     2161 tttctttaca tttgtaccca gcatgatccc ggacggcaac atatccgaat ctaccggcac
     2221 taccctatcc tacaccccgc aagccctgct caatgcggtg ggtaattccc tccaatcccc
     2281 gctgttcaat gaggttttcc gtataaaagg gatatacaaa tatggcaaag gcgtcaacta
     2341 taacggcgtc tattacgaca tcctccgcga cgagcaaagc gattccaaca tgaccctggt
     2401 gacccccgaa cggatgcgga ccgctctcag ggacggccag ctgatagagg cctccgcttt
     2461 tctttcccga cgtctccaga ccgccacttc gaggatcgat ctgctgctca cgctcaatga
     2521 actattttcc cggaaggcga gcatggtgag tgaagaagag accagggccc tagccctgct
     2581 gaggcgaaag gccgccatcg gctaccggga tgccgattcc tttatccggc atcgcctgtt
     2641 tggccagcaa ccgatccgga tcaccatcct ggtcggcacg accgccatcg tcgatcagga
     2701 catcaaacac caggtcaaag aagccgccgt ggcctaccgg atggaatatg tacggatcaa
     2761 tctcgcccag ccggccgaga tcgatcgcgc gatgaagcgt cataacgata ccgatatcct
     2821 cgttatcgcg cggggcggcg gggagaacct gagggtcttc gaccttccgg aactggcgga
     2881 gacggcactc gagattcgtc ctattttcat cactgccatc ggtcacagta cggacgagcc
     2941 gctgcttcag aaggtggcgg acaagtcctt tattacgccg acagcgctgg gacagtatct
     3001 tcgcgacgtc tatgaccggg cgatggacga gttcaatgat tcgaaggcaa aactgatcgg
     3061 cgatctgacc aaacagatcg atctcaacta ccaggcccgg ttgcaggatc aacaaacccg
     3121 gttgcaggac ctgcagacca ggctgacgga atcatcccgg accaaccagg aggcgatccg
     3181 tcattcggag gaaaggttgc agaccttttc gaggcaattg tcaaaaaccc ggaatttcaa
     3241 ccaatggctg atcgcgctgt tggtcgcatc gatactgatc tcgctttatc tttgggtgtc
     3301 ggtgcggcac ggtgggtgag cgtgggtagc ggtgggtgaa cctgggccgc ggtggtgagc
     3361 gtgggtcgtg gtctttcaat gcaggctatt cgttcctcaa cttcttcgcc ggattttcat
     3421 tggccgtacg aatgccctgc catccgacgg tggcaagcgc gatcgcaagc gccagcaagc
     3481 cccccgccgc aaaaacggtc cagctcaaca agacatgata gataatatta tccagccagg
     3541 catgcatggc ccagaaggcc acggggactg ccagcacaaa tgccaaaaac accggtcgca
     3601 gaaaatccgc cgagagcagc agcaatacct gccgaacgct cgcaccgaag accttgcgga
     3661 tactcatttc ccgggcccgc cgttcggcta cgaacccggc cagacccagc aggcccagac
     3721 aggagataaa gatggcgagg ccgccgaaga tcgtcgccag ggtcccatag ttattgaact
     3781 cttcgaaccg ccgctgatat tcttcttttg taaaactata atcgaatcca tgtcgtgggt
     3841 ccagggtctt caccaccgat ccgataccgg cgatggtctg ccgccagtga tcgttattgg
     3901 cgatccgaac aaagaaatgt ccgttccccg gcgctccctt atataaatag atcgccatcg
     3961 gggcgatgat gccggaggga ttattggtga caaaattctt cactaccccg atgatcgggc
     4021 tggcacccaa cttttgaccg agtaccggct catgtaaacc aagccgcgtc acggtggact
     4081 cattgaccag acaggcggag gtatcggtgc cgaatgccgg gtcgaaatcg cggccctccg
     4141 ccaaagacaa tcccgcggtc tttacccaat tgtatcccac ggtggtgatg aggatcgaaa
     4201 tttcatgtcc ggggatcttg cccggccagt tcatacctgt aacggcgccg ggaaaattca
     4261 ccaggttctc gctgcccgcg gaaatactcc ttaccccggg aaggcggctc acttcattcg
     4321 taaacagatg gtatttccct cccagatccg ccgaagcatg tacatcgacg agattctcct
     4381 gatcgtaccc gatggggcgg tcctttatgt attgaatttc ctgccagaac acgatggtgg
     4441 agatgataaa aagtatagcg atccaaaact gcgtcgtcac catcgcccgg cgaaagacgg
     4501 ctcttttcct gccgatcgtc atttctccct tcagcacttt tgcaggaagg aacttgctaa
     4561 ggaacaaggc ggggtaactt ccagcaatga taccggtgaa taaccccacc ccgatgatgc
     4621 ccgtccacac cttccaatcg aacaggtcga accggagatc cgttgccatc caccg
//
LOCUS       DASYSV010000522        21890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_491171, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000522 DASYSV010000000
VERSION     DASYSV010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 21890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21890
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491171"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VGQ51_16225"
     CDS             <1..942
                     /locus_tag="VGQ51_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV9038183.1"
                     /translation="IRIPLQQEIADKLGKVSGRTDYQLFLCNSGAEANENALKLASFH
                     TGRKKIVAFRKSFHGRTSLAVAVTDNPALVAPVNQTDNVIFLPFNDQSALETCFQQQG
                     DEIAAVIVEGIQGVGGINEASAPFLQAISKLTKKHGSVFIADSVQCGYGRSGKFFSHD
                     HAGVDADIYSMAKGMGNGFPVGGILIAPHIRPKHGMLGTTFGGNHLACAAALAVLEVI
                     QQENLIANATETGNYLMAQLKKIPQLEQVRGKGLMIGFDVPEQLRDLRKNLLWNQHIF
                     TGEARPNTIRLLPSLALRKKDADEFLEAIEEEIHAFA"
     gene            993..1955
                     /locus_tag="VGQ51_16230"
     CDS             993..1955
                     /locus_tag="VGQ51_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HEV9038184.1"
                     /translation="MQNFISVADAPDLDSLVQSALAYKADPLKDKTLGQGKRVGMLFL
                     NPSMRTRLSTQVAAQNLGAEPIVFNVDKEGWALEFEEEAIMSGNTVEHVKDAAPIMGH
                     YFDILCIRTFPSLKNKEDDYSELYIKQFIKYSGIPVVSLESATLHPLQSLTDIITITE
                     TLQGGGTRRPKIVLSWAPHVKALPQCVANSFSQWVNAWGKADFVITHPEDYELDPKFT
                     KGATITHDQDAALNNADFVYVKNWSTYNDYGKIYNNDPEWMLTNEKLSATNNAKVMHC
                     LPVRRNVELSDEILDGPSSIVTREASNRVWAAQAVLADILRTKH"
     gene            1959..2819
                     /gene="argB"
                     /locus_tag="VGQ51_16235"
     CDS             1959..2819
                     /gene="argB"
                     /locus_tag="VGQ51_16235"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEV9038185.1"
                     /translation="MQLSIIKIGGNIIDDEQRLAGFLDDFAAVPGKKILVHGGGKLAT
                     RVAEGLGIRQQLVDGRRITDAETLNVVTMVYAGAINKNIIAKLQARNCPAIGLTGADG
                     NAILSHKRAHPVTGSASPNVQAHPTTGPDSPTTPELPTIDYGFVGDVDSVNISLLTSL
                     LLLDQVLVFAPLTHDGNGQLLNTNADTIAQELARGLCSTFEVHLIYSFEKAGVLRDAE
                     DESTLIARINPSDYEDLKKKNIIFAGMIPKLDNAFTALRSGVKKVIIGKAEQLPRLLT
                     GESGTTILHE"
     gene            2812..3888
                     /locus_tag="VGQ51_16240"
     CDS             2812..3888
                     /locus_tag="VGQ51_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEV9038186.1"
                     /translation="MNNQYLYTDAIHLLEQLIAIPSFSKEEDATAALLASWLTEKNIP
                     AHRVQNNIWAKNKHWDPARPTILLNSHHDTVKPNKAYTLDPFAPISTDGKLYGLGSND
                     AGGCLVSLIATFLHFYDRTDLSHNLILGISAEEEISGHNGIELLLPSLGCAIEWAIVG
                     EPTRLQLAVAEKGLLVLDCVTHGKAGHAAREEGENAIYKALEDIGWFRTYKAPKVSDL
                     LGPVKMSVTVIDTDNKAHNIVPPLCHFVVDVRVNECYTFEELLDIIRTSTSCEVTPRS
                     LRMRSSGIPLDHPLVQSGVRLGHRCYGSPTTSDKALMPFPALKTGPGDSARSHTADEY
                     IFVEEIAAGIDNYIHLLKPIIQSQ"
     gene            3885..5195
                     /gene="argH"
                     /locus_tag="VGQ51_16245"
     CDS             3885..5195
                     /gene="argH"
                     /locus_tag="VGQ51_16245"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV9038187.1"
                     /translation="MSGNKLWSKSSTSTSALIESFTVGRDKEFDVLLAEYDVLGSLAH
                     TKMLGSVGLLSKQDLDLIQKGLNDILADIRSGSFTIDASVEDVHSQVEYLLTTRIGEA
                     GKKIHSGRSRNDQVAVDIKLYLRAQVLLLKDEVKELFDLLIKLSNQYKDSLLPGYTHL
                     QIAMPSSFGLWFGAYAESLIDDLELLSAAYQVTNKNPLGSGAGYGSSFPLDRTMTTGL
                     LKFRTLNYNSVYAQMTRGRTEKAVSMGLSSVAATIGRLAMDCTLYLNQNFGFISFPSE
                     LTTGSSIMPHKKNPDVFELIRAKCNRIQSTPNELTLLTANLPSGYHRDLQLTKEILFP
                     AFKDLEACLEMTRLMLSNIEIKEGILEDEKYKYLFSVEAVNQLVNKGVPFREAYKQIG
                     DQIDRGQFHFDYHQQLHHTHEGSLGHLCNDLISAEMDRVLAKFA"
     gene            5273..6979
                     /gene="pgm"
                     /locus_tag="VGQ51_16250"
     CDS             5273..6979
                     /gene="pgm"
                     /locus_tag="VGQ51_16250"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEV9038188.1"
                     /translation="MALSPFAGQLPAPDQLANIPSIISAYYTGIPDPSAPDQRVAFGT
                     SGHRGGSLRNSFNELHILAVTQAICNYRKQKGIDGPLFMGMDTHALSTPAHSTALEVL
                     AANDISTMIAPNDDYTPTPAVSHAILKYNEDRTKGLADGIIITPSHNPPDDGGFKYNP
                     PNGGPADTDITKWVESAANTLLEAAVKGDTSGIKRIPLARALKASTTHRHDYLNNYID
                     DLSNVLDMDAIRGGKAKVCVSPLGGAGVHYWEPIAQKYHLDLTIIDAFVDPTFRFMPL
                     DHDGKIRMDPSSPYAMNALLKMRNRFDLVVACDTDHDRHGIVTRQAGLLPPNHYLCVC
                     IDYLFRNRPGWSPSAAIGKTLVSTSLIDRVAAGLPSVPSSPGSSTLVPRRIQEVPVGF
                     KWFVSGLLDGSLGFVGEESAGAAFLRSNGKTWTTDKDGFIPALLSAEIMSRTGRDPGE
                     IYKSLTEKYGSPVYDRVDAPANAAQKKALSQLNPQQITQKELAGEPIRSMLTTAPGNN
                     APIGGLKVSTDNGWFAARPSGTEDIYKIYAESFHGDDHLRRILRDAQAIVDKAIAGDP
                     AP"
     gene            7046..9598
                     /gene="glgP"
                     /locus_tag="VGQ51_16255"
     CDS             7046..9598
                     /gene="glgP"
                     /locus_tag="VGQ51_16255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEV9038189.1"
                     /translation="MNTVKMHYGNPLYDSKAAEIEGFDSLVSLALDMRWSWNRLADEL
                     WKQLDPELWEQTFNPWLVIQSVSGEQLRHLFGTPTFRDKLDSLLRDREEEDAQPSWFH
                     TNYPNAQLKTVAYFSMEFMLGESLPIYVGGLGNVAGDQLKSASDLGVPVIGVGLLYQR
                     GYFRQFIDTDGEQVAYHPFNDPGQLPVTPLRLPNGEWLRLEVRLLGRPFWLRAWQARV
                     GRATLFLLDSNDPANSPDRREITAEVYGGDSEMRIMQEVVLGIGGWRLFEQLGISPEV
                     CHLNEGHAAFAILERAHDYMEASGSNFETALAVTRAGNLFTTHTAVPAGFDRFDPALV
                     ARHLGPYAQHRLRIPLDDLLALGRIDPHDQNEPFNMAYLAVRGSSAVNGVSKLHGAVS
                     RHLFEPLFQRWPTEEVPIHHVTNGIHTPTWESQAASRLWTEAIGQDRWKDTTESFSDA
                     IKNVSDEKIWNMRRDARKSLIDYIREHQATHFVSCGAPPEVIDKARRLFDPGVLTIGF
                     ARRFVAYKRPDLLLSDPDRLARLLADYKQPVQLVIAGKAPPYDDSARDLIRRWIMFIR
                     NYGLEDRVVFLDDYDMLMAGYLVGGMDVWLNTPQRPWEASGTSGMKVLVNGGLNLSEL
                     DGWWVEAYRPEVGWALGDGLEHGNDPTVDRQEAQALFALLEQQVIPQFYHRNANGIPV
                     EWIKKVRESMATLTPFFSSNRTVREYTEKFYLPAATTYQARAAQNSDHGKKIVEWKHN
                     LEQHWPNLGFGRAKVETKDNMYVFNVQVYPHGLDPASMTVQLVAAAPQGNGHLFVAEM
                     LRSGQADAAAGWIDYCAWVPATRPIADYTARIIPHNAEVAIPLEYDRIIWQH"
     gene            9735..11306
                     /locus_tag="VGQ51_16260"
     CDS             9735..11306
                     /locus_tag="VGQ51_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV9038190.1"
                     /translation="MRKTCSILFFAASISVTTLHAQTHGPSAPASYTPGPPVPTSSAP
                     SSSAHAPDDAKTRTLSGIQMPDSSSSKTRAISDIQAHYDEYKNIALRIWDFAEVGYKE
                     VKSSALLQKTLSDAGFTVRNGVADIPTAFVATYGSGSPVIGILAEFDALPGLSQQAIP
                     EKAPIDGQTAGHGCGHHLFGTASVAAGIEIKNLISEHHLTGTIRVYGCPAEEGGSGKV
                     YMVRAGLFNDVDVVIHWHPGSDNSVTLTSALANKSAKFRFHGLAAHAAAAPDKGRSAL
                     DGVEAMDYMVNMMREHIPQETRIHYVITNGGKAPNVVPDFAEVYYYVRHPKKEEVVRI
                     FDWVTKAAEGAALGTATTVDYEVIGGTHDLLLNRTLARAMQQNLQRVGGVSYTPEEIG
                     FAKKIQTSFTYSVPPIASAATITPLRETNDAGGGSTDVGDVSYVVPTVGLTTATWVPG
                     TPAHSWQATACGGTDIGIKGMMVAAKTMALTAIDCFTNPSLITAAKEEFRKDKGDYQY
                     KALLGDRKPALNYRD"
     gene            11311..12252
                     /locus_tag="VGQ51_16265"
     CDS             11311..12252
                     /locus_tag="VGQ51_16265"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEV9038191.1"
                     /translation="MSTQKHHPHHTHGSEYPTPPSPGPTSEHSATNPEHSATHSTTPE
                     HTATHPTPPEHAASHPAPPEHTATHPAPPEHTATHPATSEHPAPSPEITLEKEFELER
                     MILFTDAVFAIAITLLIIDIKWPELHPEQKGAQLFNFFAPTIFGFVAFATSFVYIGRS
                     WSVHLRLFRLIKKYDQGLINRNLFFLFFIVMFPFANSGLTQHNAYAYVIPVFIYLANL
                     GFLGIAHYRLCRYIILEKPSLSVEGHRAEKKFVYARGRSMMFITCATPIIGVGLWLLF
                     PEHPFYILYSFVFLFLGLRVVKRRLRALYPKDTDNEA"
     gene            complement(12370..13647)
                     /locus_tag="VGQ51_16270"
     CDS             complement(12370..13647)
                     /locus_tag="VGQ51_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV9038192.1"
                     /translation="MRITVIAFILLMAATRMNAQKIDSVQVRATESILASDAMRGRAT
                     FTPDIERAADYIESRFKHAGLKTWNGSDSYRQSFAMVRTTPVSATATLADSVLPAGHV
                     MALSSASDLQVDQNSGYEKAYIRAGANFVMEVLHYLHEKKNYVVFVDTSFTSSFARVG
                     RFGRQQFKTDRSIVFVLTSSDPSTWSVHVEQQVKESPLANVVGILPGHGEKKDEIVIF
                     SAHYDHLGVGKPDEKGDSIFNGANDDASGTTAVLMLADYFAKKKHNARTLVFATFTAE
                     EIGEFGSQYFSGQFNPDSVVAMFNIEMIGTESKWGRNSAYITGYERSDMGNILEKNLT
                     GSGFSFHPDPYPDQNLFYRSDNATLARLGVPAHTISTSKMDSEKYYHTRGDEIGTLDM
                     SNMAQIIKAIAISSTSIVNGKDTPSRVDTAKMR"
     gene            complement(13663..14397)
                     /locus_tag="VGQ51_16275"
     CDS             complement(13663..14397)
                     /locus_tag="VGQ51_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9038193.1"
                     /translation="MIKAIIIDDERLARNELKKLLMDFPEIEVIAEAANAAEGVEKID
                     SLNPDLIFLDIQMPGKTGFDMLAELERTPNVIFTTAYDEYALKAFEVNALDYLLKPVE
                     PKRLADALQKLQVEEDKEPLSDHTISVNRSILNEHDQVFVKDGERCWFVKLSDIRLFE
                     SVGNYAKVYFGPNKPLILKSLNALEERLDEKVFFRANRKHIVNLRLIEKIEPYFNGGL
                     LLEMKGGEKIEVSRRQTVKFKEMMSL"
     gene            complement(14443..15495)
                     /locus_tag="VGQ51_16280"
     CDS             complement(14443..15495)
                     /locus_tag="VGQ51_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9038194.1"
                     /translation="MTKNGSKYYWWCQFGGWGSLAMVLVLNSSTFDQKITNRQIQIMV
                     ITVLTGIFVTHLFREVIKRNGWTLMTLEKAAPRFIIGMLATCILSALIRIGVADALSL
                     ASHRGSEFLPRLFTLAADTGVMIVPWTLIYYLYHYVLISSKQQVDTLKLEAMVKELEL
                     KTIKAHINPHFIFNALNSIRALIDENPVRARTAITELSNILRSSMQAEKLETVTFEKE
                     LNIVKDYLALEHIRFEDRLQVEYVINEDTLDQPVPPMMLQTLVENAIKHGISRQVDGG
                     KVRIISDFTDDFYHKLVIQNTGVLNGSRDSDGFGLASTKNRLQLLFGQKANFDIRAVN
                     ENMVEAKVQIPVGADK"
     gene            15708..17633
                     /gene="dxs"
                     /locus_tag="VGQ51_16285"
     CDS             15708..17633
                     /gene="dxs"
                     /locus_tag="VGQ51_16285"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEV9038195.1"
                     /translation="MEITPGPLLSTIDSPDNLRQLTREQLHQLSDELRQYIIDTVSVH
                     GGHFAASLGVVELTVALHYVFQTPYDQLVWDVGHQAYGHKILTGRRDNFATNRKYNGV
                     SGFPRRSESPYDTFGVGHSSTSISAALGMAMAAKYKGEDDRKTVAIIGDGAMTAGLAF
                     EGMNHAGVADTDLLIILNDNCMSIDPNVGALKEYLTDISTSRTYNRLKDDIWKALGKL
                     PGSTFSREIASKIEHSIKGFVSRSSNLFEALNLRYFGPIDGHNISKLVDTLEDMRHIP
                     GPKLLHIMTVKGKGYALAEKDQTKWHAPGLFDKITGEIYKKHYPTPQPPKYQDVFGLS
                     IIELAERNPKIFGITPAMPSGSSLKFMMEKMPDRAFDVGICEQHAVTLSAGMATQGMR
                     VYCNIYSSFMQRAYDQVIHDVAIQDLPVIFCLDRAGLVGEDGPTHHGAYDIAYMRCIP
                     NLIVSSPMNESELRNLLYTAQLDTNHHPFVIRYPRGEGVMPEWKTPFQEIPVGKGRKL
                     KDGEDIAILSLGHPGNFATAAIRELRTTGIDPAHYDMRFVKPLDAQLLHEVFQRYDRI
                     LTVEDGTVLGGFGSAILEFQAAHGYNATVRILGIPDRIVEHGSPKELQHECGYDAPAI
                     AGAVREMMKDKVRVTTS"
     gene            17674..20751
                     /locus_tag="VGQ51_16290"
     CDS             17674..20751
                     /locus_tag="VGQ51_16290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9038196.1"
                     /translation="MRASFICGIAAFLTILSAHPTYAQQYYFARYTPRNGLVNNRVRA
                     LYQDHRGRIYFATYGGLSVYDGARFINYTAADGLATGLVNDVVEMGDDSLWVIPNARV
                     IHCLVHGILKDVATSDHFYPVINQLLRASDGHYYAISDDGLFRFENNRFIHVPLKDAH
                     GKEMGKYLNHAIESHQRLLIVNDPNLENIVHAGALFVYDPATGKCAISPDTIAIASVI
                     ETPEHDILIGTATGVRRLDTTALRQEVIRPLPPPEHYAEVSGLIGDYMYFDHSGNLWI
                     SSPDAVVRFGTNGDRQIFGPSNGLPAGVVNYILQDRENNMWFANEQNGVVKLVGQQVQ
                     YYSSLRPGFTVTDLSADGTGDSVWFYDMMHHDLLLVQGDKQTIFHSASPLPFPTNIAI
                     GKKAYMVSDHDIYALRFQGHRFYADPVWHDTSHIDGRVFFSRSGDLVDPGMQMAVFTG
                     ATMLRQPLSNLADQGAVDRSGHIWVVTRSNELFVYGIDKDRRSLRLLRHYGKGTLPPW
                     SPRSLAIDTTGRVWIGTRDRGMYCMRSSGDSLVFERQLTMQDGLSENFINFLHCDPEG
                     NIWASSPTGLDRVRLRNGRVIIDNITLSSDRYDAVYKVVSAAGNICWALARGGVMKIS
                     RLAGEAGDYSPNVLFSSIQVGDEPVTAFPTDPAAGPATDPSIPSANPSNPSANPSTPS
                     ANSSTPSAAGSRTPLTLPYNRNTLSFFVGAPTFINEGLTRYSWRLEGSRDPKWSTPSS
                     RPDIGLVNLPPGKYTLHVKAQFLTGRYPDKTAAWSFIILPPWWQTWWFRTLFVLACAA
                     VIAALIRIYIRSRLEIQRTRLEKKQAVEKERTRIATDMHDDLGAGLSRIKFLSETIHM
                     KKQMGQPVQDDVGSIGRYANEMIDKMGEIVWALNEKNDSLNDLLSYTRSYAAEYLEQS
                     GIPCTLDAPPADGVSRFVSGEFRRNVYLTIKEALHNIVKHSKARNVTIRMIVGKDLTI
                     VIEDDGVGFDPAGVRPSGNGLNNMRHRVNDIGGQLQIRHVDGTVIRLTVPLPD"
     gene            21000..21662
                     /locus_tag="VGQ51_16295"
     CDS             21000..21662
                     /locus_tag="VGQ51_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9038197.1"
                     /translation="MPITIVIVEDLDEVREGLGNFLSLDTDLSLTGAYKTADEAAQGI
                     ITLKPDVVIMDINLPGTNGIDCIRQIRSLVPATQFMMFTVYADDEKVVEALQAGASGY
                     LLKSTGLPHIIEALKELHRGGSPMSANIARKLVKHFQQPPRTIPSPENSVLSPRETDI
                     LHLLARGLLYKEIANQLSVSTSTVRQHIHHIYEKLHVQNRTEAINKVYRNDADSTRPP
                     RL"
BASE COUNT         4963 a         7282 c         5203 g         4442 t
ORIGIN      
        1 atccgcatcc ctctgcagca ggagattgcc gacaaattgg gaaaggtcag cggccgcacc
       61 gattaccagc tattcctctg caattccgga gccgaggcaa atgagaacgc cctcaagctc
      121 gcctccttcc acaccggccg gaaaaagatc gtcgccttcc gcaaatcctt tcatggccgt
      181 acctcactgg cggtagccgt taccgacaac ccggctctcg tcgccccggt caaccagacc
      241 gataacgtga tcttcttacc cttcaatgac cagtccgcgc tcgaaacctg tttccaacaa
      301 cagggcgacg agatcgccgc agtaatcgtc gaaggcatac agggcgtagg cggcatcaat
      361 gaggcttccg cacctttcct ccaggccatc agcaagctca ctaaaaaaca cggcagcgtc
      421 ttcatcgccg acagcgtaca atgcggctat ggccgcagcg gcaaattctt ctcgcacgat
      481 catgccggcg tcgacgctga catctactcg atggccaaag gcatgggcaa cggtttcccg
      541 gtcggtggca tcctcatcgc gccgcatatc cgccccaaac acggcatgct gggcaccacc
      601 ttcggcggca atcacctggc ctgcgccgca gcgctggcgg tgctcgaagt catccagcag
      661 gagaacctga tcgccaacgc caccgaaaca ggcaactacc tgatggctca gctcaaaaag
      721 ataccgcagc tggagcaggt gcgcggaaag gggctgatga tcggtttcga cgtccccgag
      781 cagctcaggg acctccgcaa gaatcttctc tggaaccagc atatctttac cggtgaggcc
      841 aggccgaata ccatccgtct gctgccttcg ctggccctcc gcaaaaaaga tgcggatgag
      901 ttcctcgaag ccatcgaaga agaaatccac gcattcgcct gatcacatgg cggcccgccc
      961 gaagggcggg ccgcccccat aacgcgcata acatgcaaaa cttcatctcc gtcgccgatg
     1021 cccccgatct cgatagcctc gtgcaatccg ccctcgccta taaggccgat ccgctgaaag
     1081 acaaaaccct cggccagggc aaacgcgtgg gcatgctctt cctcaacccc agcatgcgta
     1141 cccgcctctc tacacaagtg gctgcgcaaa acctcggcgc cgaacccatc gtcttcaatg
     1201 tcgacaaaga aggctgggcc ctcgaattcg aagaagaagc gatcatgagc ggcaataccg
     1261 tcgaacacgt caaagacgcc gcccctatca tgggtcatta cttcgacatc ctttgcatcc
     1321 gtaccttccc ctcgctgaag aataaagagg acgattacag cgagctctat atcaaacaat
     1381 tcatcaaata ttcgggtatc cccgtcgtga gcctcgagag cgcaacgcta cacccgctgc
     1441 aaagcctcac cgacatcatc acgatcaccg aaacgctgca gggcggcggc acccgccggc
     1501 caaagatcgt cctctcctgg gcgccccacg tcaaagccct cccccaatgc gtcgccaaca
     1561 gcttctccca atgggtcaat gcctggggaa aggcggactt cgtaattaca caccccgaag
     1621 actacgagct cgacccgaaa ttcacgaaag gcgccaccat cacccacgac caggacgccg
     1681 cgctcaacaa cgccgatttc gtctatgtaa aaaactggag cacctacaac gattacggta
     1741 agatctacaa taacgacccc gaatggatgc tcaccaacga aaagcttagc gccaccaaca
     1801 acgcgaaggt catgcactgt cttcccgtcc gccgcaatgt cgagctcagc gacgagatcc
     1861 tcgacggccc ctccagcatc gtcacccgcg aagcctccaa ccgcgtatgg gcggcccagg
     1921 ccgttctggc ggacatactc cgtaccaaac actaacccat gcagctctcc ataatcaaaa
     1981 taggcggcaa tatcatcgac gacgagcaac ggctcgcagg ctttctcgat gacttcgcag
     2041 ccgtgccggg aaaaaagatc ctcgtccacg gcggtggcaa actggccacc cgggtagcgg
     2101 aaggcctggg catccgccaa cagctggtcg acggccggcg catcaccgac gccgaaaccc
     2161 tcaacgtcgt caccatggtc tatgccggcg ccatcaataa aaatatcata gcaaagctgc
     2221 aggcccgcaa ctgccccgcc atcggcctca ccggcgctga cggtaatgcc atcctcagcc
     2281 acaaacgcgc gcaccccgtg accggttcgg cctcccccaa cgtccaggcc catccaacga
     2341 ccggcccgga ttctcccacc acccccgagc ttccaaccat cgactacggt ttcgtgggcg
     2401 acgtcgactc cgtcaacatc agcctcctta cgagccttct cctcctcgac caggtactcg
     2461 tcttcgcccc ccttacccat gacggcaacg gtcagctgct caacaccaac gccgatacca
     2521 tcgcccagga actcgcccgc ggcctctgct ccaccttcga agtacacctc atctattcct
     2581 tcgagaaagc cggcgttctt cgcgacgccg aggacgaatc caccctcatc gcccgtatca
     2641 acccctccga ctacgaagac ctcaaaaaga aaaatattat tttcgctggc atgatcccca
     2701 aactggacaa tgcctttacc gcgctccgca gcggggtaaa aaaggtcatc atcggcaagg
     2761 cagaacagct gccccggctg ctgaccggcg aatccggaac aacgatcctt catgaataac
     2821 caatatctat ataccgacgc aatacacttg ctggaacagc tgatcgccat tccttccttc
     2881 agtaaggaag aggatgccac cgccgctttg ctcgcatcct ggctgaccga aaagaatatc
     2941 cccgcccacc gtgtccaaaa caacatctgg gcaaagaata aacactggga tccggcccgc
     3001 ccgacgatcc tgctcaattc tcaccacgat accgtaaaac ccaacaaggc ttataccctc
     3061 gaccccttcg cgcccatctc caccgacggg aaactctacg gtctcggcag caacgacgcc
     3121 ggaggatgtc tcgtctcgct gatcgcaacc tttctgcact tctatgaccg caccgatctt
     3181 tcacacaacc tgatcctggg catctctgcc gaagaagaga tctcgggtca taacgggatc
     3241 gagctgctgt tgccttcatt gggatgtgcc atcgaatggg ccatcgtagg cgaacccacc
     3301 cggctccagc tggccgttgc cgaaaaaggt ctgctcgtac tcgattgcgt gacacatggc
     3361 aaggccggcc acgccgcccg cgaagaaggc gaaaatgcca tctacaaggc cctggaagat
     3421 atcgggtggt tccgtactta caaggccccg aaagtctccg atctgctcgg ccccgttaag
     3481 atgtccgtca ccgttatcga caccgacaac aaggcccaca acatcgttcc cccgctttgt
     3541 catttcgtag tggacgtacg cgtcaacgaa tgctatacct tcgaagagct gctcgacatc
     3601 atccgcacca gcacgtcctg cgaagtcaca ccccgtagtc tccgtatgcg ctcttcgggc
     3661 atccccctgg atcacccgct tgtgcagtcg ggcgtgcggc tcggtcacag atgctatggc
     3721 agcccgacta catccgacaa ggcgctcatg cccttccccg ctctcaagac gggtcccggt
     3781 gactccgccc gcagccatac ggccgacgaa tatatctttg tcgaagagat cgccgccggc
     3841 atcgacaact atatccacct cctcaaacct ataattcaat ctcaatgagc ggcaacaaac
     3901 tttggagtaa atcttccacc agcacctccg cgctgatcga atccttcacc gtcggccgcg
     3961 acaaggaatt cgacgtcctg ctcgccgaat acgacgtact gggctcgctg gctcacacaa
     4021 aaatgctcgg gtcggttggc ctcctgtcca aacaggatct cgacctcatc caaaaaggtc
     4081 ttaacgacat actggccgac atccggtccg gctccttcac catcgacgca tctgtcgagg
     4141 acgtccactc gcaggtggaa tacctgctca ccacccgcat cggcgaggcc ggcaaaaaga
     4201 tacacagcgg ccgtagccgc aacgaccagg tcgctgtcga tatcaaactc tatctccgcg
     4261 cgcaggtcct cctgctcaag gacgaggtca aggaattgtt cgatctgctc ataaaactca
     4321 gcaaccaata caaagacagt ctccttcccg gctatacgca tctccagatc gcaatgcctt
     4381 cctccttcgg attgtggttc ggcgcctatg cggaaagcct gatcgatgat ctcgagctcc
     4441 tgtccgccgc ctaccaggtg accaacaaaa acccccttgg cagcggagcc ggctatggca
     4501 gcagcttccc tctcgatcgc accatgacca ccgggctcct gaaattccgc accctcaact
     4561 acaactccgt gtacgcgcag atgacccgcg gccgcaccga aaaagccgtg tccatgggcc
     4621 tctcctccgt cgccgcgacc atcggccgtc tcgccatgga ctgcacgctc tatctcaatc
     4681 agaatttcgg gttcatttct ttcccgtccg agctgaccac cggcagcagc atcatgccgc
     4741 ataaaaagaa ccccgacgtg ttcgagctca tccgcgccaa atgcaaccgc atccagtcga
     4801 cccctaacga gctgaccctc cttacggcca atctcccctc tggctaccac cgcgacctcc
     4861 agctcacgaa agagatcctc ttccccgcct tcaaagacct cgaagcatgt ctcgaaatga
     4921 cccgtctcat gttgtccaac atcgagataa aggaaggcat cctcgaagac gaaaaatata
     4981 aatacctctt tagcgtcgag gcggtgaacc agctggtcaa taaaggcgtc cccttccgcg
     5041 aagcctataa acagatcggc gaccagatcg atcgcggcca gttccatttc gattatcatc
     5101 agcagctgca tcatacccat gaaggcagtc tggggcatct ctgcaatgac ctcatctccg
     5161 ccgaaatgga tcgggtactg gccaaattcg cttagccacc cccccccaag attatttaac
     5221 caccgcacac cggtaaaaat cccgaactta gggtagtcaa aaccggttat tcatggcatt
     5281 aagtcccttt gctggccaac tgcccgcccc cgaccaactg gccaacatcc cgtccatcat
     5341 ctctgcctac tacacaggta tccctgatcc ttcagcgccc gatcaacgcg tcgcctttgg
     5401 cacctccggt catcgcggcg gatcgctccg caacagcttc aacgagcttc atatcctcgc
     5461 cgtcacacag gccatctgca attaccggaa acaaaaaggc atcgacggcc ccttattcat
     5521 gggcatggat acgcacgccc tgtctacgcc cgcgcactcc acggcactcg aagtcctcgc
     5581 cgcaaacgat atatccacta tgatcgcccc aaacgacgat tataccccca cccccgccgt
     5641 ctcgcacgcc atcttaaaat ataacgaaga ccgcacgaaa ggccttgccg acggcatcat
     5701 catcacgcct tcgcataacc cgcccgatga cggcggcttc aaatacaacc cgcccaacgg
     5761 cggtcccgcc gatacggaca tcaccaaatg ggtcgagtcc gccgccaaca ctctgctgga
     5821 agccgcggtc aaaggcgaca cttcaggcat caaacgcatc ccgctcgccc gcgccctgaa
     5881 ggcctccacc acccatcggc acgattatct taacaattat atagacgatc taagcaacgt
     5941 gctcgatatg gatgccattc gcggcggcaa ggccaaggtc tgtgtctctc ccctcggtgg
     6001 agcaggcgtc cattactggg agcccattgc gcaaaaatat cacctcgacc tcaccatcat
     6061 cgacgccttt gtcgacccta cgttccgctt catgccgctc gatcacgacg gcaagatccg
     6121 tatggacccg tcttccccat atgcgatgaa cgcgctgtta aaaatgcgca accgctttga
     6181 cctcgtcgtc gcctgcgaca ccgaccatga cagacacggc atcgtcaccc gtcaggccgg
     6241 tctgctgcct cccaatcact acctgtgtgt ctgtatcgat tatcttttcc gcaaccggcc
     6301 cgggtggtcc ccatccgccg cgataggcaa gacccttgtc agtacttccc tcatcgaccg
     6361 tgtcgctgcc ggcctcccat ccgtcccctc ttccccgggc tcctccaccc tcgtcccgcg
     6421 ccgtatccag gaagtgcccg tcggtttcaa atggttcgtg agcggcctcc tcgacggcag
     6481 tctcggcttc gtgggagaag aaagcgcagg cgccgcattc cttcgcagca acggaaaaac
     6541 ctggaccacc gataaggacg gcttcatccc cgccctgctc tccgcggaaa taatgtcccg
     6601 caccggtcgg gatcccggcg agatctataa gtccctgacg gaaaagtacg gttcacccgt
     6661 ttatgatcgc gtcgacgccc cggccaacgc cgcgcaaaaa aaggcgctct cgcaattgaa
     6721 cccgcaacag atcacccaaa aagaactcgc cggcgagccg atccggtcca tgctcactac
     6781 cgcgcccggc aacaatgcgc ccatcggcgg gctcaaagta tccaccgaca acggctggtt
     6841 cgccgcccgc ccttccggca ccgaggatat ctataagatc tatgccgaaa gttttcacgg
     6901 tgacgatcac ctgcgccgca tcctccgcga tgcacaagcc atcgtcgata aggccattgc
     6961 cggcgacccg gcaccataac gccaatcctc atcccacaaa ccccctccgg ctttgcctct
     7021 ccctcttcac actaaaagac attttatgaa caccgtcaaa atgcactacg gcaatccact
     7081 ctacgactcc aaagccgcag agatagaagg attcgattct ctcgttagtc tcgcgctcga
     7141 catgcgctgg tcctggaaca ggctggcaga cgaattatgg aaacagctgg atcccgaatt
     7201 atgggaacag accttcaatc cctggctggt aatccaaagc gtatccggtg aacaattgcg
     7261 gcatcttttc ggaacgccta cctttcgcga caagctggat tccctgcttc gcgaccgcga
     7321 ggaagaagac gcgcaaccca gctggttcca taccaactac cccaacgcgc agctgaagac
     7381 cgtcgcctat ttcagtatgg aattcatgct cggcgagtcc ctccccatct atgtcggagg
     7441 acttggcaat gtggccggcg accagctgaa gtcggccagc gatctgggcg tacccgtcat
     7501 tggcgtcggc ctgctttatc aacgcggtta tttcagacaa ttcatagata ctgacggcga
     7561 acaggtggcc taccatccct ttaacgatcc cggtcaacta ccggtgacgc ctctgcgcct
     7621 gcccaacggg gagtggttgc gtctcgaggt gagactgctc ggcagaccgt tttggctgag
     7681 ggcctggcag gcacgtgtcg gaagagcgac cctctttctt cttgatagta acgatcccgc
     7741 caactcgccg gatcggcgcg agatcacggc cgaggtctat ggaggcgatt ccgaaatgcg
     7801 catcatgcag gaggtcgtcc tgggtatcgg cggctggaga ttattcgaac agctgggcat
     7861 atcccccgag gtctgccacc tcaacgaagg gcatgccgca ttcgcgatcc tcgaacgcgc
     7921 ccacgattat atggaagcct ccggctctaa tttcgagact gccttagcag tcacacgcgc
     7981 cggaaatctt ttcacgacac acacggccgt cccggccgga ttcgaccggt ttgaccctgc
     8041 tctcgtcgca agacatctcg ggccatatgc acaacacagg ctcaggatcc cgttagacga
     8101 cctgcttgca ctcggacgca tcgacccgca cgaccaaaat gagcctttta acatggccta
     8161 tctggccgtc cgcggcagct cggccgtcaa tggcgtcagc aaactgcacg gagctgtaag
     8221 ccggcatctg ttcgaaccgc tgttccaacg ctggcccacc gaggaagtgc cgatacacca
     8281 tgtcactaac ggtatccaca cccccacgtg ggagtcccag gcggccagcc ggctgtggac
     8341 tgaggcgatc ggacaggaca ggtggaagga taccaccgag tcgttcagcg atgccatcaa
     8401 aaatgtgtcc gacgaaaaaa tatggaacat gaggcgggac gcgcgcaaat ccctgatcga
     8461 ttatatccgt gaacaccagg ctactcactt cgtctcctgc ggcgccccgc cggaggtcat
     8521 cgacaaagcg cgccgtctct tcgacccggg tgtgctgacc atcggattcg cgcgtcgctt
     8581 cgtggcctat aaaagacccg atctgctgct ctcggacccc gaccgcctgg cgcgtttgct
     8641 cgccgattat aaacaaccgg tccagctggt catcgccggc aaagcgcccc catacgatga
     8701 cagcgcccgc gacctcatcc gccgctggat catgttcatc cggaattatg ggctcgaaga
     8761 tcgggtcgtc tttctcgacg actacgacat gcttatggcc ggttatctcg taggcggcat
     8821 ggatgtatgg ctcaataccc cgcagcgtcc ctgggaagcc agcggcacca gcggcatgaa
     8881 ggtgctcgtc aatggcggtc tcaatctgtc cgagctggat ggatggtggg tagaagctta
     8941 ccgccccgaa gtcggatggg cactgggaga cggtctcgaa catggcaatg accccactgt
     9001 tgatcgacag gaagcccagg cgctttttgc attgctcgaa cagcaggtga tcccgcaatt
     9061 ctatcatcgt aacgccaacg gtatacccgt cgaatggatc aaaaaagtgc gcgagagcat
     9121 ggcgaccctt acgccattct tctcttccaa ccgcaccgtc cgcgagtaca ccgaaaaatt
     9181 ttacctgccg gccgccacga cataccaggc ccgcgccgcc caaaacagcg atcatggtaa
     9241 aaagatcgtc gagtggaaac ataacctcga acagcattgg cccaacctcg ggttcggccg
     9301 ggcgaaagtg gagactaagg acaacatgta tgtcttcaac gtacaggttt atcctcacgg
     9361 tctcgacccc gcatcgatga ccgtgcagct ggtcgccgcc gctccccagg gcaatggcca
     9421 cctgttcgtc gccgaaatgc tgcgttccgg ccaggccgac gccgccgcgg gttggatcga
     9481 ctactgcgcc tgggtccccg cgacccggcc cattgcagac tatacggccc gtatcatccc
     9541 gcacaacgcc gaagtggcga tcccgctgga atacgaccgc atcatctggc aacactgaat
     9601 gatccgccgc ccctgcgctc acgccgcatc cccgcgacgc accccctcac gcccgcgccc
     9661 gcaccgccgc gacccatctg cttttcgcgt ccttccaaaa aaagcaatta ccttgtaccc
     9721 acaacctact gcatatgaga aaaacctgca gcatcctctt tttcgccgca tccatcagcg
     9781 taaccactct ccatgcccaa acccacggcc cttccgcccc cgcctcttac acccccggtc
     9841 cccccgtccc cacctcttcc gccccgagct catccgccca tgcgccggac gacgccaaaa
     9901 cccgcaccct ctccggcatc caaatgcccg actcctcttc ctccaagacc cgcgccatct
     9961 ccgacatcca ggcgcactac gacgaataca agaatatcgc cctccggatc tgggacttcg
    10021 ccgaagtcgg ctataaagaa gtaaaaagct ccgcgctcct tcaaaaaacc ctctccgacg
    10081 ccggcttcac cgtccgcaac ggcgtcgccg acatccccac cgccttcgtg gcgacttacg
    10141 gttccggcag ccccgtcatc ggcatcctcg ccgaattcga cgcgcttccc ggactctccc
    10201 aacaggccat ccccgaaaag gctcccatcg atggccaaac agccggtcat ggttgcggcc
    10261 atcatctctt cggcacggcc tccgtcgccg caggcatcga aataaagaac ctcataagcg
    10321 aacatcatct caccggcacc atccgcgtat atggatgccc ggcagaagaa ggcggctccg
    10381 gaaaagtata catggtccgc gccggtctgt tcaacgacgt cgatgtcgtc atccactggc
    10441 accccggctc cgacaattcc gtcacgctca ccagcgccct cgccaacaaa tcggctaaat
    10501 tccgttttca tggtctggcc gcacacgctg cagcggcccc ggacaaaggc aggtccgcac
    10561 tcgatggcgt tgaggcgatg gattatatgg tcaatatgat gcgcgaacat attccgcagg
    10621 aaacgcggat acactatgtc atcaccaacg gcggaaaggc gcccaacgta gttcccgact
    10681 tcgccgaggt ttactattat gtccgccatc cgaaaaaaga agaggtcgtc cgcatcttcg
    10741 actgggtgac aaaggcggca gaaggcgcag ccctcggcac cgcgaccact gtagactatg
    10801 aggtcatcgg cggtacccac gacctgctgc tgaaccgaac gctagcccgg gcgatgcagc
    10861 aaaatctcca gcgtgtcggt ggtgtctcct atactccgga ggagataggg ttcgcaaaaa
    10921 agatccagac aagcttcacc tattccgttc cccccatcgc atccgccgcc accatcacac
    10981 cgctcaggga gacaaacgat gccggaggcg gctcgaccga cgtaggagac gtcagctatg
    11041 tcgtcccgac agtaggactg accactgcta cctgggtccc cggcaccccc gcccacagct
    11101 ggcaggccac ggcctgcggt ggcaccgaca tcggcatcaa aggcatgatg gtcgccgcaa
    11161 aaactatggc cctcaccgcg atcgattgct tcaccaaccc atcgctcatc acggccgcga
    11221 aagaagagtt ccgtaaagac aagggagact atcagtataa ggcattgctc ggagaccgca
    11281 agcccgcgct taactataga gactaaacat atgagcaccc aaaaacacca ccctcatcat
    11341 actcacgggt ccgaataccc gacgccccct tctcccggcc ccacgtccga acactccgcg
    11401 accaatcccg aacattccgc gacccattcg accacacccg aacacaccgc cacccatccg
    11461 acaccccccg aacacgcggc ctcccatccg gcacctcccg aacacaccgc cacccatcca
    11521 gcaccccccg aacacaccgc cacccatccg gccacgtccg aacacccggc cccctctccc
    11581 gaaatcacac tcgaaaaaga attcgagctc gagcgtatga tcctcttcac cgacgcggtc
    11641 ttcgccatcg ccatcaccct gcttatcatc gatatcaaat ggccggagct ccacccggaa
    11701 caaaaagggg cccagctgtt caatttcttt gcacccacaa ttttcggatt cgtagccttc
    11761 gccacgagct tcgtctacat cggccgctcc tggtcggtac acctgcggct gtttcgactc
    11821 ataaaaaaat atgatcaggg gctcatcaac cggaatctct ttttcctctt cttcatcgtc
    11881 atgtttccct tcgccaattc cggtctcacc caacacaatg cctatgcgta tgtaatacca
    11941 gtctttatct atctggccaa tctgggcttt ctcggcatcg cccactaccg tctttgccga
    12001 tatatcattt tggagaaacc ttcgctgtcg gtggaaggtc acagggcgga aaagaaattc
    12061 gtctatgccc gcggccgtag tatgatgttc atcacctgcg ccacaccgat catcggcgtc
    12121 ggattgtggc tgctcttccc ggaacatccg ttttatatcc tatacagttt tgtcttcctt
    12181 ttcctcggcc tgcgtgtcgt caagcgtcgc ctgagagccc tatatcctaa ggataccgat
    12241 aatgaagcat agggcgttcc ccgcctccgc aaagctgcgg cgggtcgggc tctccgctat
    12301 agtcctcgcc tatcggctcc gggctgccgc ttcgatccct aacgcagtcg ggaagaccat
    12361 tcagccgccc taacgcattt tcgccgtatc cacccgcgac ggcgtatcct tcccattcac
    12421 tatcgaagtc gagctgatcg ctatcgcctt gatgatctgg gccatattgg acatatccag
    12481 cgtccctatc tcatccccgc gcgtatgata atatttctcc gaatccatct tcgaggtgga
    12541 gatggtatgc gccggcactc ccaaccgcgc cagcgttgca ttatcgctcc ggtaaaacag
    12601 gttctgatcc ggatagggat ctggatggaa cgaaaaaccg ctgcccgtta gattcttttc
    12661 caggatatta cccatatcgc tccgttcgta tccggtgata tacgccgaat tccggcccca
    12721 cttgctttcg gtgccgatca tttcgatgtt gaacatcgcc acgacgctgt cgggattgaa
    12781 ctgacccgaa aaatattgcg aaccgaattc cccgatctcc tccgcagtga aggtcgcgaa
    12841 tacgagcgta cgcgcattgt gcttcttctt cgcgaaataa tcggccagca tcagcaccgc
    12901 ggtcgtcccg ctggcatcgt cattggcgcc gttgaagatg gagtcgcctt tctcatccgg
    12961 cttgccgaca cccaggtgat cgtaatgggc cgaaaagatc acgatctcgt ccttcttctc
    13021 gccgtgtccc ggcagtatgc ccaccacatt ggccagcggc gattccttca cctgctgttc
    13081 gacatggacc gaccatgtcg acggatcgct actcgtcagc acaaaaacaa tactccgatc
    13141 tgttttgaac tgctgcctgc caaaccgccc gaccctcgca aaggaagagg taaaggaagt
    13201 gtccacgaat acgacatagt tctttttctc atgcaaataa tgcaggacct ccattacgaa
    13261 attcgcgcct gcccggatat acgccttctc atatccgctg ttctgatcca cctgcagatc
    13321 gctggcgctg ctcagcgcca tcacatgacc cgccggcagc acactgtcag ccagcgtggc
    13381 ggtcgccgat acaggtgtgg tcctcaccat cgcgaaggac tgccgatagc tgtccgatcc
    13441 gttccaggtc ttcagccccg catgtttaaa tcttgattcg atataatccg ccgcccgctc
    13501 gatatcggga gtgaaggtcg cgcggccccg catggcatcg gacgccagta tgctttcggt
    13561 ggctcttacc tgtacgctgt cgatcttctg cgcattcatg cgggtcgcag ccattaacag
    13621 gataaaggcg ataacagtga tcctcatatg tagtggttga gtttacagac tcatcatctc
    13681 tttgaacttg accgtctggc gacggctgac ctcgatcttt tctccgcctt tcatctccaa
    13741 caacagcccg ccgttgaaat aaggctctat cttttcgatg agacgcagat tgacgatatg
    13801 tttgcggttg gcccggaaaa acactttctc atccaacctt tcctccagtg cgttcagcga
    13861 tttcaatatc agcggtttgt tgggcccgaa atataccttc gcataattcc cgacgctttc
    13921 gaaaagccgg atatcggata gcttcacgaa ccagcaccgc tcgccgtcct ttacgaatac
    13981 ctggtcatgc tcgttcagta tgctccggtt gaccgagatc gtatgatccg aaaggggttc
    14041 cttgtcctcc tccacctgca gcttctgcaa ggcatccgcc aggcgtttgg gctccaccgg
    14101 tttcagcagg tagtccagtg cattcacctc aaacgccttt aaggcatact cgtcatacgc
    14161 cgttgtaaag atcacattgg gcgtccgctc cagctcggcg agcatgtcga aacccgtttt
    14221 gccgggcatt tgtatgtcga ggaagatcag atcgggattg agcgagtcga tcttttccac
    14281 gccttcggcg gcattcgccg cctcggcgat cacttcgatc tccgggaaat ccatcaataa
    14341 tttcttcagc tcattgcggg ccagacgctc gtcatcgata ataatagctt tgatcatgtg
    14401 caatattttc aacggcgcgg ccttcggccg cgcctccgat tattatttat ccgcccctac
    14461 cggtatctgc actttcgcct ccaccatatt ctcattcacc gcgcggatgt caaaattcgc
    14521 tttctgcccg aacaacagct gcagcctgtt cttcgtactg gccaggccaa aaccgtcgga
    14581 atcgcggctg ccattcagca cccccgtatt ctggatcacc agcttatgat aaaaatcatc
    14641 cgtaaaatcc gagatgatcc tcactttacc gccatcgacc tgccggctga tgccatgctt
    14701 gatcgcattc tcgacaagtg tctgaagcat catgggcggc accggctgat ccagcgtatc
    14761 ctcattgatg acatattcca cctggagccg gtcttcaaaa cggatatgct ccagcgccag
    14821 atagtccttt acgatgttca gctctttttc gaaggtcacc gtctccagct tctccgcctg
    14881 catactgctc cgcaggatat tgctcagctc ggtgatggca gtacgcgcac gcaccgggtt
    14941 ctcgtcgatc agcgcccgga tgctgttcag ggcattgaaa ataaaatgcg ggttgatatg
    15001 cgctttgatc gtcttcagct ccagttcttt taccatcgcc tccagcttca gcgtatccac
    15061 ctgctgtttg ctgctgatca gcacataatg atatagataa tagatcagcg tccagggaac
    15121 gatcatcaca cccgtatccg ccgccagcgt gaacagacgc ggcaggaact ccgaccctct
    15181 atgcgatgcc aggctcaaag catccgctac cccgatccgg atcagcgcgc tgaggataca
    15241 ggtcgccagc atcccgatga tgaaacgggg agcggccttt tccaacgtca tcaatgtcca
    15301 gccgttacgt ttgatcacct cgcggaacag atgggtgacg aaaatacccg tcaacacggt
    15361 gatcaccatg atctggatct gtctgttcgt tatcttctgg tcgaatgtgg atgaattcaa
    15421 caccaggacc atggccaaag acccccaacc gccaaattgg caccaccagt aatatttgct
    15481 tccgtttttg gtcatatcgc tactatcgct tcttaaggta aggcaaatta ggcttttccc
    15541 cgccctccca cccccgattt tgtactaatg gctaatttac ccctgccatc ggtccagtac
    15601 gttccacggc ctccccccga aaattaacaa ttttcggggg ataaggtgta tttttacatg
    15661 tcggggcggg tcgtgggtcc gcccgcactg caaaatctga agaagacatg gaaatcaccc
    15721 ccggacctct cctaagtacg atcgattcgc ccgataacct gagacaactg accagagagc
    15781 agttgcacca gctaagcgac gaattgcgac aatatatcat cgacaccgtc agtgtccatg
    15841 gcgggcattt tgccgccagc ctgggagtgg tcgagcttac cgtggccctg cactatgtct
    15901 tccaaacccc ttatgatcaa ttggtctggg acgtcggcca ccaggcctac ggacacaaga
    15961 tcctcaccgg ccgacgggac aatttcgcca ccaatcgcaa atacaacggg gtcagcggct
    16021 tcccgagacg ttcggaaagc ccctacgata cattcggggt cgggcactcc tctacctcca
    16081 tcagcgccgc cctgggaatg gcaatggccg ccaaatacaa gggagaggac gaccgtaaga
    16141 ccgtagccat catcggcgac ggcgccatga cggcgggtct cgccttcgaa gggatgaacc
    16201 atgcgggcgt ggcggatacc gatctgctca ttatcctgaa tgacaactgc atgagcatcg
    16261 accccaacgt gggcgcgctc aaggaatatc tcaccgatat cagcacctcc cgcacctata
    16321 acaggctgaa ggacgatatc tggaaagccc tcggaaaact ccccggcagc acattcagcc
    16381 gcgagatagc cagcaagatc gagcacagca tcaagggttt cgtcagccgc agcagcaatc
    16441 tcttcgaggc gctcaatctt cgttatttcg gtcccatcga cggacacaat atcagcaagc
    16501 tggtcgatac cctcgaggat atgcggcaca tccccggccc caagttgctg catatcatga
    16561 cggtaaaagg aaaaggttac gcgctcgccg aaaaagatca gaccaaatgg catgctccgg
    16621 gtctcttcga caagatcacg ggcgaaatct ataaaaaaca ctatcccacc ccgcagccac
    16681 ccaaatacca ggatgtcttc ggtctcagca tcatcgagct ggccgaacgc aacccaaaga
    16741 tcttcggtat cacgccggcc atgcccagcg gatcatcgct caaatttatg atggagaaga
    16801 tgccggaccg cgccttcgac gtgggtattt gtgaacaaca tgccgtgacc ctcagcgccg
    16861 gtatggccac acagggcatg cgggtatatt gcaatatcta ttcttctttc atgcaaaggg
    16921 cgtacgatca ggtgattcat gatgttgcca tccaggacct ccccgtcatc ttctgtctcg
    16981 atagggcagg tctcgtcggc gaggacggac ccacgcatca cggcgcctat gacatcgcct
    17041 atatgcgttg catacccaat ctcatcgtca gctcgccgat gaatgaaagc gaattgcgca
    17101 atctcctcta tactgcacag ctcgacacga atcatcatcc cttcgtcatc cggtatcccc
    17161 gcggtgaagg cgtcatgccg gaatggaaga ctcccttcca ggaaataccg gtcggaaagg
    17221 gccgtaagct aaaagatggt gaagacatcg ctatcctgag cctgggccat ccgggtaatt
    17281 tcgccaccgc agcgatccgc gaactgcgga ccacaggcat cgaccctgcg cactacgata
    17341 tgcgctttgt caagccgctc gatgcgcagc ttctgcacga agtctttcaa cgatacgatc
    17401 gtatcctcac cgtcgaagac gggacggtac tcggaggatt cggctcggcc atcctcgagt
    17461 tccaggcagc ccacggctat aatgccaccg tacgcattct tgggataccc gaccgcatcg
    17521 tcgagcacgg ctcgccgaag gagcttcagc atgaatgcgg ttatgacgcg ccggctatcg
    17581 ccggcgccgt ccgcgaaatg atgaaggata aggtccgcgt caccacctcg taaccacgaa
    17641 agccattctt ccgaccctgc atcaatatat agtatgcggg catcgttcat ttgcggcatc
    17701 gccgcattcc tgaccattct ttctgcacat cccacctatg cgcagcaata ttattttgcc
    17761 cgctatacac cacgcaacgg cctcgtcaac aaccgcgtcc gcgcccttta tcaggaccac
    17821 cgtggtcgta tctatttcgc cacttatggc gggcttagcg tttatgacgg cgcgcgtttc
    17881 atcaattata ccgcggcgga cggcctggcg accggtctgg tcaacgacgt agtcgagatg
    17941 ggtgacgatt cactctgggt catccccaat gcccgggtga tccattgtct tgtccacggc
    18001 atcctgaagg atgtcgccac cagcgatcat ttttatcctg tgatcaatca gctgctgcgg
    18061 gccagtgacg gccattacta cgctatcagc gacgacgggc tgttccgttt cgagaacaat
    18121 cgctttattc acgttcccct caaagatgcg catggcaaag aaatgggcaa atacctcaac
    18181 catgccatcg agtcgcacca acgcctgctc atcgtcaacg atcccaatct cgaaaatatc
    18241 gtccacgccg gcgcactctt tgtctatgat ccggcaacgg gcaaatgcgc catctctccg
    18301 gataccatag cgatagcttc cgtgatcgaa acccccgaac acgatatcct catcggtacg
    18361 gccaccggtg tccgacggct ggatacgacg gccctccggc aggaggtgat ccggcccctg
    18421 ccacctcccg aacattatgc cgaagtgtcg gggctgatcg gcgactacat gtacttcgat
    18481 cactccggca atctctggat atcgtctccc gatgcggtag tgcgtttcgg aacgaacggc
    18541 gacaggcaga tctttggtcc gtccaatggc ctcccggccg gtgtcgtcaa ttacatcctc
    18601 caggaccgcg agaacaatat gtggttcgcc aacgaacaga atggggtagt caagctggtc
    18661 ggccagcagg tgcaatacta ttcttcctta aggcccggtt ttacagtgac cgatctgtct
    18721 gccgacggca ccggggattc ggtatggttc tatgatatga tgcaccacga tctgctgctc
    18781 gtgcagggcg acaaacaaac gatcttccac agcgcgtcac cattgccttt ccccacgaac
    18841 atcgccatcg gaaagaaggc ctatatggtt tcggaccacg atatctatgc gcttcgcttc
    18901 cagggccacc gcttctatgc cgatcccgtc tggcatgaca ctagtcatat cgatggcagg
    18961 gtgtttttca gccggtccgg agacctggtg gacccgggaa tgcaaatggc tgtcttcacg
    19021 ggcgcgacga tgctccgtca gccgctgtcc aatctcgccg atcagggggc ggtcgaccgg
    19081 tcgggtcata tctgggtcgt cacccgaagc aatgaattat tcgtctacgg catcgacaaa
    19141 gaccggcgtt cactacgact cctgcgccat tatggcaagg gaacattacc gccctggagt
    19201 ccgcgttcgc tggccatcga caccacggga agagtgtgga tcggcacccg cgaccgcggt
    19261 atgtattgta tgaggagctc cggcgacagc ctggtattcg aacgacagct gacgatgcag
    19321 gacgggctct cggagaattt catcaatttc ctccactgcg accccgaagg aaatatctgg
    19381 gccagtagtc ctaccggtct cgacagggtc cgtctccgga atgggcgggt catcatcgac
    19441 aatatcactc tttccagcga tcggtatgat gccgtttaca aggtcgtgtc cgccgcggga
    19501 aatatctgtt gggcactggc acgcggaggc gtcatgaaga tctcgcggct ggccggagaa
    19561 gcaggcgatt attcaccgaa tgttcttttt tccagcatcc aggtcggcga cgaacccgtc
    19621 acagcctttc ccaccgaccc cgccgcaggc cctgctaccg acccctcgat cccctccgcc
    19681 aatccctcaa acccttcagc caacccctca actccctccg ccaattcctc aaccccctcc
    19741 gccgccggct cccgcacccc cctaaccctg ccctataacc gcaataccct ctcctttttc
    19801 gtcggtgcgc ccaccttcat caacgaaggc ctcacgagat attcctggcg gctcgaagga
    19861 agccgcgatc cgaaatggag caccccctcc tccaggcccg acatcggcct tgtcaacctc
    19921 ccgcccggga aatataccct gcatgtcaaa gcgcaattcc tgaccggccg ctatcccgat
    19981 aagacggctg cctggtcctt catcattctc ccgccctggt ggcagacctg gtggttccgg
    20041 accctgttcg tactcgcctg cgcggccgtc atcgccgcac tcatccgtat ctatatccga
    20101 agccggctgg aaatacaacg cacgcggctc gaaaagaaac aggccgtcga aaaagaaagg
    20161 acgcggatcg ccaccgatat gcatgatgac ctcggcgcgg gcctctcgcg gataaaattc
    20221 ctcagtgaga ccattcacat gaaaaaacag atgggccagc ccgtccagga tgatgtaggc
    20281 agcatcggcc gttatgccaa tgaaatgatc gacaaaatgg gggagatcgt ctgggccctc
    20341 aacgagaaga acgactcgct gaacgacctg ctttcctata cgcgctccta tgcggccgaa
    20401 tatctcgaac aatcaggcat tccctgcacc ctcgatgcgc cgcccgcgga cggcgtttca
    20461 cgtttcgtca gcggcgaatt ccggcggaat gtatatctca ccatcaaaga agccctgcac
    20521 aatatcgtaa agcattcgaa ggcccgcaac gtgacgatac ggatgatcgt aggcaaagac
    20581 ctcaccatcg tcatcgaaga cgacggcgtc ggattcgatc cggccggcgt ccggccctcc
    20641 ggcaacggac tcaacaatat gcggcatcgc gtaaacgaca tcggcggcca actgcagatc
    20701 cgccacgtcg acggtaccgt catccggctt acggtgcccc tgccggacta acccatcccc
    20761 cccaccgccc cgccccattg tcccccacgc ttcatcgggc accccgcact ctcccacgct
    20821 ttcagctcca ccccgcgctt ccacaagtac tccgcgcttt cagccccaac ccacgcttca
    20881 tcgggcaccc cgcactttcc cccgtaactt tcgtactatt tcaggatatt gcaattgatc
    20941 atacattcac cgtctcacac cgccatcatg cccatatcca ttgtcataac gcctccgcca
    21001 tgcccatcac cattgtcata gtggaggatc tcgacgaggt ccgggagggc ctggggaact
    21061 tcctctccct ggataccgat ctgtccctca ccggcgccta taaaaccgcc gacgaggccg
    21121 cacagggcat catcacactg aaacccgatg tcgtgatcat ggatatcaat ttgccgggaa
    21181 ccaacggcat cgactgtatc aggcagatca gatcgctggt gccggctacc cagttcatga
    21241 tgttcaccgt ctatgccgac gatgagaagg ttgtcgaagc cctccaggcc ggcgcatcgg
    21301 gatacctgct gaaaagcacc ggtttgccgc atatcatcga ggccctgaaa gagcttcatc
    21361 gcggcggatc cccgatgagc gccaacatcg cgcgcaaact ggtaaaacac ttccagcaac
    21421 ccccccgcac cattccatcc cccgaaaatt cggtcctttc cccccgtgaa acggacatcc
    21481 tccacctatt ggcccgcgga ctcctgtata aggagatcgc caatcagctc tccgtcagca
    21541 ccagtacggt ccgccagcat atccatcata tctatgagaa gctacacgtg caaaaccgta
    21601 ccgaagccat taataaagta taccgcaacg acgccgattc gacccgtccg ccccgcctct
    21661 aacttttgtt ctattgcccg cggttcttcc tttttatact ttgaagaaaa aaaatatgaa
    21721 accgagcatt ttgttcctgc tcctgctaac ccctttcgcc cttttcgccc agcagcaacc
    21781 gcaacaatct ccccctccca aatcgcaaag ctccggtggc ggcatcggta tagggatacg
    21841 gggaggtttc aatttcgcca acgtcaccaa tgcgtcggag gtcaatggca
//
LOCUS       DASYSV010000523         5468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2287136, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000523 DASYSV010000000
VERSION     DASYSV010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287136"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VGQ51_16300"
     CDS             complement(<1..581)
                     /locus_tag="VGQ51_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HEV9038198.1"
                     /translation="MDIAPPSVSFDNTANAFEYKSDKELRKAAFLFSSMGYSALVKIG
                     TMVTPWLIRSGMPVKGLIRRTIFTQFVGGETLEETAAVARKLGGYHVQVILDYGVEGG
                     EEGEKGFDHACGEFIRVIEHAATEPNIPFMSVKVTGFARMGLLEQIDAAMAVRTGAGG
                     NEGDRGEGEGTVMKRYARALEGLSAETREEWQAV"
     gene            637..1824
                     /locus_tag="VGQ51_16305"
     CDS             637..1824
                     /locus_tag="VGQ51_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEV9038199.1"
                     /translation="MEFNHLDAIPTITSPYLCARRSVKPVNPSPMTILHTDIYKFSIP
                     MHPFTIATGTMHYAQNILIRLHTDAGYYGVGECSAFPMIVGETQATCFEMAQDFARLW
                     KGKEALDIQTRMRELHDFTAFNATIKSAFDMALHDLAAKAADQPLYRFLGGKKRAVET
                     DLTIGIDTPGRMAEKAIKFKTDGVRMIKIKLGKNPKEDVERVRLIRAAVGDEIILRID
                     ANQGWDFDSAHDALRLMGDSNVQFCEQPMRRFNDHRLPGLRQSSPVKIMADESVFDHH
                     DALRLIAADACDYVNIKFAKSGGILEATAIDAVCAEHHIPCMMGGMLESRVALTAFMH
                     FALAHDNIIFHDMDTCMLGHTADPVTGGVRYNGYFLEISDEPGLGADADDAFLKKCGS
                     ITV"
     gene            1858..2379
                     /locus_tag="VGQ51_16310"
     CDS             1858..2379
                     /locus_tag="VGQ51_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEV9038200.1"
                     /translation="MQNNTKTAKESLIVMTELVLPNDTNVFGNLMGGRLMYWMDIAAA
                     LSAGKHCNAPVVTASVDNISFENPIKLGNVVHIEAKVSRAFNTSIEVHMKVWGEDHTQ
                     QYKYKSNEAYYTFVALDPNRRPRTVPALVAETEEEIRLFDGALRRRQLRLILGGKMKP
                     EEAGELRAFFVKS"
     gene            2661..3326
                     /locus_tag="VGQ51_16315"
     CDS             2661..3326
                     /locus_tag="VGQ51_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038201.1"
                     /translation="MLFSLSSPPALAQQLPDSAFLRQNLHDELGPDSRLYNGYEYIRN
                     GTPAKGFPFFETDSLQKAALSYDGILYRDIPLEYDLVLDKVIIRDYTGKSLISLISEK
                     VDDFSFGTRHFRYIIAIDNPGFYEVLLSSGPITLLARREKKLNFPSNREDQARYDQRN
                     SYYLRIGNNFYQVDGKTALLDALKDKKDALKKFISANKLHFKKQLETSLTRTTAYYTH
                     LSH"
     gene            3328..>5468
                     /locus_tag="VGQ51_16320"
     CDS             3328..>5468
                     /locus_tag="VGQ51_16320"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9038202.1"
                     /translation="MRHRLSHSLLLALLLFAVINAQAQQTKRVTGNFEGYSFSRLMAR
                     LEALTGYHFFYDPADVDSLSIDLNVNNATVPQILDQIFQNTDFHYAIDPAGRVFITRR
                     VTILTTLPAQLGGARKPTDTSAVLLSEQQEKAAGVKKAFAENQLIDIGNKANRSGGKA
                     IIAGYVRNGATGEAIVGATLHADTLSLTVTTDQFGYYSVTLPKGRHVLRITDAGMKDT
                     RRQVMVYSDGKLDIDMQDAVTTLKAVIVSAEKTSNTQSVHMGVNRLNIKSIKQVPVVF
                     GETDVMKVVLTLPGVSSVGEASNGINVRGGSTDQNLILFDDATVYNPSHLFGFFSAFN
                     PDVVKGIELYKSAIPEKYGGRLSSVLDVSMLDGNSKKWSGIAGIGPLTGKFMIEGPLQ
                     KEKTSLVAAVRTTYSNWLLNSLPANSGYSNSRANFYDANLRITHVIDPNNTLYLMGYL
                     SSDKFNLDNDTTYRYKNKNAVIKWKHIFNNRSFALFTAGWDHYEYNVSDDRDSVSAFN
                     LGFRIDQSHFRADFSYTPGNRHSFSYGLSTVYYQLHPGSYSPVGDGSKVVNTVVPPEQ
                     GLESAIWLGDQYTVNSRLSISAGLRYSMFNYMAPHDLFNYVPGQPRDVSTITDTSRYT
                     KGVIRTYTAPEIRLSARYTLSDAASLKFSFNTLEQYIHMLSNTVAISPTDIWKLSDPH
                     IRPQQGDQFSLGYYQNFKSNTIEASLEVYYK"
BASE COUNT         1303 a         1810 c         1209 g         1146 t
ORIGIN      
        1 acggcctgcc actcttcacg cgtttccgcc gacaaccctt ccaacgcccg ggcataacgc
       61 ttcatcaccg tcccttcgcc ttcaccccgg tcgccttcgt tccctccggc tcccgtcctt
      121 accgccatag cggcatctat ctgctccagc agacccatcc gcgcaaaacc cgtcaccttc
      181 acgctcatga agggaatatt gggctcggtg gccgcatgct cgatgacccg gatgaattcc
      241 ccgcaggcat gatcgaaacc cttttcacct tcttcgccac cttccacccc ataatccagg
      301 atcacctgca catggtatcc tcccaatttc cgcgcgacgg cagccgtctc ctccagcgtc
      361 tcccctccca caaactgcgt gaagatcgtc ctccttatca acccttttac cggcatcccg
      421 cttcggatca gccacggcgt caccatggtc ccgatcttca ccagggccga ataacccatg
      481 ctggaaaaaa gaaaggccgc tttcctgagt tctttatcgg atttgtattc gaacgcgttg
      541 gcagtgttat cgaaagagac agacggtggg gcgatatcca tatagtcaaa caataatttg
      601 ccggcaaatt tatctatttt cgaataatcg gccttcttgg aatttaacca cctcgacgca
      661 atccctacca ttacttcccc ttatctttgc gcgcgcaggt ccgtcaaacc cgtcaaccca
      721 tcgccgatga ccatcctcca tacagatatc tacaagttct ccatccccat gcaccctttt
      781 acaatcgcta cggggactat gcactatgcg caaaacatcc tgatccggct acacacggat
      841 gccggctatt acggtgtcgg cgaatgctcg gcattcccta tgatcgtcgg ggaaacacag
      901 gccacctgtt tcgagatggc tcaggatttc gcgcggctgt ggaaaggcaa agaggccctg
      961 gatatccaaa cccggatgcg cgagctacac gacttcacgg ccttcaatgc gacgatcaaa
     1021 agcgctttcg atatggcgct ccacgacctg gccgcaaagg ccgccgatca gcccctatat
     1081 cgattcctgg gaggcaaaaa aagagccgtc gagaccgatc tgaccatcgg gatcgatacc
     1141 cccggcagaa tggccgaaaa agctattaaa ttcaagaccg acggcgtgcg tatgatcaag
     1201 atcaagctgg ggaagaaccc caaagaagac gtcgaacgcg ttcgccttat ccgagcggcc
     1261 gtgggcgatg agatcatcct tcgcatcgac gccaaccagg gttgggattt cgattccgct
     1321 cacgacgccc tgcgactgat gggtgactca aatgtccagt tctgcgaaca acccatgcgc
     1381 cggttcaacg accatcggct gcccgggctc cggcaaagct ccccggtaaa gatcatggcc
     1441 gacgaaagcg tcttcgacca ccacgacgcc ctcaggctca tcgccgccga cgcatgcgat
     1501 tatgtaaata taaaatttgc aaaatcgggc gggatcctcg aggccactgc catcgatgcg
     1561 gtttgcgccg aacaccatat cccctgcatg atgggcggaa tgctggaaag ccgcgtcgcc
     1621 ctcaccgcct ttatgcattt tgccctcgcg cacgacaata tcatctttca tgatatggat
     1681 acctgcatgc tgggacatac cgccgatccc gtcaccggtg gcgtacgcta caacggctat
     1741 ttcctggaga tatccgatga gcccggtctg ggcgcggatg ccgacgacgc ctttctgaaa
     1801 aaatgcggga gcatcacggt ctagcgctag tctttcattt ctgtctatat ttgccctatg
     1861 caaaataaca caaagacggc aaaagaatcc cttatcgtca tgaccgagct ggtcctgccc
     1921 aatgacacca acgtattcgg caacctcatg ggcggccgcc tcatgtactg gatggatatc
     1981 gccgccgccc tgtctgccgg caaacactgc aacgcccccg tagtcaccgc ctccgtggac
     2041 aatatctcct tcgaaaaccc tatcaaactg ggcaatgtcg tgcacatcga ggccaaggtc
     2101 agccgcgcct tcaacacctc catcgaagtc catatgaagg tctggggaga agaccatacg
     2161 cagcaataca aatataaaag taacgaggcc tactatacct tcgtggccct cgatcccaac
     2221 cgccgccccc gcacagtccc cgcgctcgtg gccgagacgg aagaagagat ccgcttattc
     2281 gacggcgccc tccgcagacg ccagctccgt ctcatccttg gcggaaaaat gaagcccgaa
     2341 gaagccggcg aacttcgcgc cttcttcgta aaaagctgaa gcctcggcgc catcacctcc
     2401 cgttctcatt cttccccgcg gtacctccag cgccatcact gcccgttctc attcttcccc
     2461 gcggtacctc cggcgccatc acctcccgtt cgcattcttc cccgcggtac ctccccctcc
     2521 cctcaatacc cacattccgg aattcccggt ccatatcccg gtatcccatg cccgaataag
     2581 aggaatttgt cctaacttca tcacaaagct tgtcattcat gcgcgtcacg ctttcattca
     2641 aaactattgg ctgcttcctt atgctctttt ccctatcctc cccaccggcc cttgcccagc
     2701 aattgcccga ctcggctttc cttcggcaaa atcttcacga tgagctcggc cccgacagcc
     2761 ggctctacaa cgggtacgaa tatatccgta acggcacccc cgcaaaaggc ttcccctttt
     2821 tcgaaaccga tagtctgcaa aaagcagcgc tttcctatga cggtatccta tatcgcgata
     2881 tccccctgga atatgacctc gttctcgata aggtgatcat ccgggactac accggcaagt
     2941 cgctcatcag ccttatcagc gaaaaggtcg acgacttttc cttcggcacc cgtcatttcc
     3001 gctatatcat cgccatcgac aatccgggct tttacgaggt actcctctct tccggcccca
     3061 tcaccctcct ggcccgccgc gaaaaaaaac tcaacttccc ctccaacagg gaggatcagg
     3121 caagatatga ccagcggaac tcctactacc tgcgcatcgg caacaacttc tatcaggtag
     3181 acggcaaaac cgccctcctc gacgccctca aagacaaaaa agacgccttg aaaaaattca
     3241 tcagcgccaa caaactccac ttcaaaaaac aattggaaac gtccctcacc aggaccacgg
     3301 cctattacac gcacttaagt cattgatatg cggcaccgcc tctcccattc tttattgctc
     3361 gctctcctgc tctttgctgt catcaacgcg caagcccaac agacgaaaag ggtcaccggc
     3421 aatttcgaag gctattcctt ttccaggctg atggcccgtc tcgaagccct caccggctat
     3481 catttcttct atgaccccgc agatgtcgac agcctcagca tcgatctcaa cgtcaataac
     3541 gctaccgttc cgcagatact cgatcagatc ttccagaaca cggatttcca ttatgctatc
     3601 gatccggccg gacgcgtgtt catcacccgg cgcgtaacga tcctgaccac cctcccggct
     3661 caactgggcg gcgcccgaaa acccaccgac accagcgccg tgctgctgtc cgaacaacag
     3721 gaaaaagcag ccggggtgaa aaaagccttc gccgagaacc aactcatcga catcggcaat
     3781 aaggcgaacc gctcgggagg caaggccatc atagccggct atgtccgcaa tggcgccacg
     3841 ggcgaggcca tcgtcggcgc cactctccac gcggataccc tgtccctcac tgttacaacc
     3901 gaccagttcg ggtattattc ggttaccctt ccaaaaggcc gccacgtcct ccggatcacc
     3961 gacgcaggca tgaaggatac ccgccggcag gtcatggtct actccgacgg caaactggac
     4021 atcgatatgc aggacgccgt gaccactctc aaggcagtca tcgtctcggc ggaaaaaacc
     4081 tccaatactc aaagcgtcca catgggcgtc aacaggctaa atataaaaag catcaaacag
     4141 gtgcctgtcg tattcgggga gacagacgtc atgaaggtcg tcctcacgct gccgggcgtc
     4201 agctccgtcg gcgaggccag caacggcatc aatgtccgcg gcgggtccac agaccagaac
     4261 ctcatcctct tcgatgacgc gaccgtctat aatccatccc atctgttcgg cttcttctcc
     4321 gcattcaatc ccgatgtggt caaggggatc gagctataca agagcgccat cccggaaaaa
     4381 tacggcggtc gcctttcatc tgttctcgat gtctcgatgc tcgacggcaa cagcaaaaaa
     4441 tggtccggga tcgccggcat cggaccgctc acaggaaaat tcatgatcga aggcccttta
     4501 caaaaagaaa agacttcgct cgtagccgcg gtccgcacta cctattccaa ctggctgctc
     4561 aattccctgc cggccaacag cggctatagc aacagcaggg ccaatttcta tgacgccaac
     4621 ctgcgcatca cacatgtcat cgatcccaac aacacgctat atctgatggg atacctcagc
     4681 tccgacaagt tcaacctcga caacgatacg acctaccggt acaagaataa aaatgctgtc
     4741 atcaaatgga agcatatttt caacaataga tccttcgcgc tgttcaccgc cggctgggat
     4801 cattatgaat acaacgtctc cgatgaccgc gattccgtca gcgccttcaa cctgggcttc
     4861 cggatcgatc aatcccattt ccgggccgat ttcagctata cgcccggcaa ccggcattca
     4921 ttcagctatg gcctgagcac ggtctattat cagctgcacc cgggttccta ctcgcccgtc
     4981 ggcgacgggt cgaaggtggt caataccgta gtacccccgg aacagggtct cgaaagcgcg
     5041 atctggctcg gcgaccagta taccgtcaat tcacggctct ccatcagcgc cggactgcgc
     5101 tatagcatgt tcaactacat ggccccgcac gacttattca attatgtccc ggggcagccg
     5161 cgggacgtta gcaccatcac ggacaccagc cgctatacaa aaggggtcat caggacctat
     5221 acagctcccg agatccggtt gtccgcccgc tataccctct cggatgctgc atcgctcaaa
     5281 ttcagcttca ataccctcga gcagtatatc cacatgcttt ccaacaccgt cgcaatctcg
     5341 ccgacggaca tctggaaact cagcgatccc catatccggc cacagcaggg tgaccagttc
     5401 tccctcggct attatcaaaa tttcaaatcc aacaccatcg aggcctcact cgaagtgtac
     5461 tataaaca
//
LOCUS       DASYSV010000524        17424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1770631, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000524 DASYSV010000000
VERSION     DASYSV010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 17424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17424
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770631"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VGQ51_16325"
     CDS             <1..1194
                     /locus_tag="VGQ51_16325"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HEV9038203.1"
                     /translation="AEYGPDFEHQILRDRNHPSVFMWSIGNEEWMIQTSTTGKLIAQT
                     QIQRQRLLDPTRTCTYAANVGNVYHGVNEVIPVRGFNYNLGGLDGYRRDHPDQPIMGT
                     EVASTVSTRSIFEKDTVNAYVPDYDSVYPPWASTAEQWWRIAADRPWFMGGFAWTGFD
                     YRGEPTPFGWPDINSHFGIMDMCGFPKTVYYYYQSWWTDKDVLHIAPHWNWPGTMTGH
                     EIPVWVNSNADNVELFLNGKSLGKKDMPRNGHLEWMVSYAPGKLEAVAYKKGRKLTAV
                     VETTGEPYRIVVKASKPALGNEPEAAIVYNCSVVDRQGREVPDASNLLHFSVTGNVRI
                     IGVGNGNPSSHEPDQFKEGGWQRHLFGGRCQVIIESAGPAWDADRASLIVSAQGLPSE
                     KMYMQ"
     gene            1195..2208
                     /locus_tag="VGQ51_16330"
     CDS             1195..2208
                     /locus_tag="VGQ51_16330"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9038204.1"
                     /translation="MKLPRPIAKQFKVAAYSSPAISALTVTPLFLLRNIPFELYPWTF
                     GIAALLVFIFWTVNIGIYRMVSRDARDRNRVRYLASYAFCILFSFFLFHDLFQEHFGS
                     SRSGLHFHVIIFFAVDTVILILQDLVVTREKNVRIELENSRLHMRDMEAMNLKLAQQV
                     HPHFLFNSLSTLKALISVAPEQATEYLVRLSGFLRSSMTDKGVVRVGRELELCGDYLA
                     MQQIRFGEALRYSIEVPEEVRGAYFVPVFSVQLLIENAIKHNVLTPDRPLSIDIVYRD
                     GVITVNNNLQRKAAGSAGNASGLINLRERYQALGGDPVRIEETTDLFSVSIKLLIHEY
                     GDH"
     gene            2189..2929
                     /locus_tag="VGQ51_16335"
     CDS             2189..2929
                     /locus_tag="VGQ51_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9038205.1"
                     /translation="MNTVIIEDEPLMAQDLKACILASEPSARVVAILSSVREAIGYFR
                     EQPPPDLIFSDIQLGDGLSFSIFAANEQTIPVIFCTAYDEYALDAFRAAGIDYILKPY
                     SSKDIAAALAKYRTFRGEAPSYDEIARSFGKRKQQSVLVYYKEKIVPVDVGDIALFYL
                     HNDLTRLLTFGKQQYVVSRSLEELERTIAGRFYRVNRQFLVNRRAIIDVSQYFGRKLL
                     LNLSIPFDEKVTVGRLRTNSFLEWLAGE"
     gene            complement(3175..4008)
                     /locus_tag="VGQ51_16340"
     CDS             complement(3175..4008)
                     /locus_tag="VGQ51_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9038206.1"
                     /translation="MKKLILSLALSTPLALCAQTSTNAHSLAKIWETSPIVNVPESVL
                     PDATHSFLFVSLIEGGPWDVDGKGAVGKLGLDGKHYDSTWITGLDAPKGLGRFGNRLY
                     VADVSNVVVMDIPKGRIIKKIAIAGAIGLNDITVDGSGVVYVSDSKAGKVYRIEKDVP
                     ALYMDHLDGANGLKATKNGLYILANKQVLFADAGKHLRTITSLPNGGDGVEEVGNGDL
                     LVSEWVGYIYYVYADGRKEMLLDTHALHKNTADICYDPSNRTLWVPGFNGLTVTAYRL
                     K"
     gene            complement(4016..5437)
                     /locus_tag="VGQ51_16345"
     CDS             complement(4016..5437)
                     /locus_tag="VGQ51_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV9038207.1"
                     /translation="MIRYLACFLLFSCSGILRAQTRPNIILILADDLGYGDVGYQGQQ
                     KIHTPNIDRMAGEGMRFTEFYAGTAVCAPSRASLMTGLHTGHTPIRGNRGFKPEGQYP
                     LPDSSLTIATVLQQHGYITGAFGKWGLGYPGSTGTPGKKGFTDFFGYNCQTLAHNYYP
                     DHLWINDEKLELPGNLKKDSVYSGDLIHQHALRFLRENHDRPFFLYLAYTLPHAALQV
                     PHDSVYRHYVAQFGESPRAPGAADKRSDYTFEPYPHAAFAAMVSRLDRYVGDILREVK
                     ELGIGGKTLILFTSDNGPHREGGGDPAFFDGSGPFRGIKRDLYEGGIREPFIALWPGT
                     IRSGSVCNRPAAFWDLFPTFEETAGLPASKHIDGYSILPLLKGMDRLARQHDYFYWEF
                     HENGGRQAVRWGNWKGVRLGVNTDPDPPIELYDLSKDPGEKENIAAQHPDIVRHIKTI
                     MLGAHLQNKDWPPTADETHGTKM"
     gene            5534..6400
                     /locus_tag="VGQ51_16350"
     CDS             5534..6400
                     /locus_tag="VGQ51_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV9038208.1"
                     /translation="MEPLFSEKIQKPSSGYFMLFVILAILVFAILLLAQPGSAGAGVV
                     CMVLFILLSKGLLVVQPNTAKVFLLFGAYRGSVRTSGLYWVNPFYGRQTLSIRARNFE
                     SDQLKVNDKLGNPVLISIILVWNVKDTYRASFAVDDYAKFVKVQADAAVRKLAASYPY
                     DHFEDERAGITLSTDFDQVNATLEHETAERLDMAGIEVVEARITHLSYAPEIASAMLR
                     RQQATAVVAARHKIVEGAVSMVESALNLLSSKNIIELDEEKRAAMVSNLMVVLCGDRE
                     AQPIVNTGTLNH"
     gene            6400..6591
                     /locus_tag="VGQ51_16355"
     CDS             6400..6591
                     /locus_tag="VGQ51_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038209.1"
                     /translation="MMAEKKPFALRMNAEMMRALERWAADEFRSVNGQIEWVLHKALR
                     EAGRMKDKPAGEKSPKEKE"
     gene            6685..7182
                     /locus_tag="VGQ51_16360"
     CDS             6685..7182
                     /locus_tag="VGQ51_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEV9038210.1"
                     /translation="MPTKQTIKAAQRDKRQGKSASTQAGEFVKAEMDRIRKGQTGARS
                     SKQAIAIGLSEARRAGVELKAPAKGKTSEKTRKNAERALAKGQRNEPIDPVRSRARTK
                     AMKKEGTEAASPRALSRQATTAAKQRSSADRSNSAKQAARTKGPAERSAAAKKAARTR
                     AQHSR"
     gene            7240..7629
                     /locus_tag="VGQ51_16365"
     CDS             7240..7629
                     /locus_tag="VGQ51_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038211.1"
                     /translation="MLKYPVTILFLLLTVAQTFSKWCLIAEFRINRDYIAQNLCVNRN
                     KPSCCCKGKCYLNKKLAEDQSQQQTPGKDARHEEIPLQVLRATNIVPVPVVTILHHRP
                     ATRHLDDEPQEYIPSFFAPPRETMFFA"
     gene            7663..9912
                     /locus_tag="VGQ51_16370"
     CDS             7663..9912
                     /locus_tag="VGQ51_16370"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9038212.1"
                     /translation="MYVFVRGLLCMGLLFVFHTGIAQTIVKGKVIDNMTGEPLENAVI
                     SAGGKGRSVLTDEQGNFEIEVAVSVDSIRASFVGYRRQSQEIDRGDESCLFRLSRSTV
                     DMRSITVQPMPNNALFSVVSHIDVNLHALNSAQDLMRLVPGLSLMQHQGGGIADHIFF
                     RGFDADHGTDVGVSVDGMPLNMVSHTHGQGFSDLHFLIPELTTSLEYGKGPYYSDHGD
                     FTTAGYLAFRTVDVPERNEVKIEAGGFHTGRIMTVVNLLGAQARQRGTSAYIAGEGFY
                     TDGPFDYAQHFNRGNLFGKYLRDLSPRTQLKVTLSTFSSLWRSSGEIPDRAVNEGLIS
                     RWGFIDSTQGGNTARSTAIVRLTTRVSDRLSWENQAYFTHYSFGLCYDQTFFADDSLN
                     GDHLLQKESRNIYGYDGKLSLHSYLPGGGVLSSSAGLGSQWNDIRGSGLSHTNAAHGV
                     LNDLQFGNIREWTLNGYIDEQYRTVHWLFDAGARLDFLDFYYDDRLNPVQPSLGRAIV
                     SPKFNMEYTFNDRSQVYLRLGKGFHSNDAKVVAEDRGIGVLPAAYGADLGINWKPFPR
                     VYVNAGIWYLALEQELTYDGDEGTFSPGDRTRREGIDLSARYQLTSWLYANADMIFCK
                     ARDAEAAKGENYLPLSVPFYGSAGLYVKLPHGFSGGWNARYMRDRPANEDNTLVAQGY
                     FLNDLTANYTTRRWEIGLEIQNLFNAKWKDAQYEVESRLKTEPQAVDDISYTPGMPFF
                     AKVKLAIFF"
     gene            complement(9945..10310)
                     /locus_tag="VGQ51_16375"
     CDS             complement(9945..10310)
                     /locus_tag="VGQ51_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV9038213.1"
                     /translation="MKRFKFLLVLLIGVATTASAQTKPQMTAVIKTPTVQCEQCKDRI
                     ETYMKHEDGIVKVNVDYKKKTTTVTWMTDRTNIENIKTAIANVGYDADDVTAEPDSYK
                     RLPTCCKKPEDGGGHAKKN"
     gene            complement(10352..11002)
                     /gene="pyrE"
                     /locus_tag="VGQ51_16380"
     CDS             complement(10352..11002)
                     /gene="pyrE"
                     /locus_tag="VGQ51_16380"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV9038214.1"
                     /translation="MGEKIITTNERAVAERLLQAQAIRLSPKEPFTWSSGWKSPIYCD
                     NRKVLGFPYIRDFVKSELCNVIFTQFPDAGLIAGVATAGIPWGAMAADQLKLPYVYVR
                     SKPKEHGLGQQIEGYYQPGQPVVVVEDLISTGKSSLQVVDILRQEGLQVVGLVSIFNY
                     GFDTAREAFERSGTPFISLSDYPTLIRLALEKGLISPQDESILLKWSADPGAWTGA"
     gene            11028..11651
                     /locus_tag="VGQ51_16385"
     CDS             11028..11651
                     /locus_tag="VGQ51_16385"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV9038215.1"
                     /translation="MYVKIYFSDKPLFLCDEMTPEIVAYSHHDDAVLIDEFDHHTVNT
                     MLHEMRQPKVHAGIFIHKDFEVLRKAFFKKFLLVKAGGGLVRNGDGKYLFMLRRGIWD
                     LPKGKLDPGETIEQCAVREVEEETGLQKVALQSPLLVTYHTYDESGKHILKETHWFRM
                     VVADGSSLKPQTEEQITELRWVADGEMKPLLQRTFPSVADVIRSANS"
     gene            complement(11629..14013)
                     /locus_tag="VGQ51_16390"
     CDS             complement(11629..14013)
                     /locus_tag="VGQ51_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038216.1"
                     /translation="MLKNYWLVAWRNLIKNKAHSLINISGLSVGMAVAILIGLWIADE
                     YSFDTQDSHYHRVARVIQNITHNGSVSTWNTVPYPLADELRKHYGADFTSVVMGIGAN
                     NHILGVGLKQMNLKGGFYEAPVTSLLDLKMLKGRGVELNDPSSMFLSASAAKALFGDS
                     DPMGQTIRLDNSFNGKVAGIYADLPYSSTFRDLQFIGGWQLYYTNMSWIKTIQDPWRP
                     NAFELYVQLTDGAAFDKVSQNIRDAKLKMLNKRLAASKPKLFLFPMSKWHLYSEFRDG
                     VNTGGRIQYIWLFGIIGIFVLLLACINFMNLSTARSEGRAREVGIRKAIGSRRQQLIG
                     QFFSESLLVAIFAFAFSLVLVGISLPFFNEIADKRMSIPWNNAWFWLTALVFTITTGL
                     VAGSYPALYLSSFHPVNVLKGNFRVGRNASIPRKVLVVLQFAVSVILIIGTIVVYLQV
                     QYAKDRPAGYDRDGLISITVSTKNIHKHFDAVQKELIGQRAIVSIAEGDGSPVNTYSS
                     TSGISWPGKDPNYFADIAFMNISWDYGKTIGWDLAAGRDFSRDFSSDTSAFILNEAAV
                     RLMGLKQPVGTIITSENIPYRIIGVVKDLVMGSPYEEVRPVVFTLNTDVNSAFIVRLT
                     PGRSVARSLAAVGAIFTKYNPDEPFQFQFVDAEYARNYENEQRIGQLAGVFACLAIFI
                     SLLGLFGMSAYMAEQRTKEIGVRKVLGASVFNLWQLLSGEFVMLVFLSLVIAIPLAWL
                     GMHTWLRHYPNRTAMTWWCFVLPAAGALLITLLTVSFQTIKAALTNPVRSLRTE"
     gene            complement(14170..14601)
                     /locus_tag="VGQ51_16395"
     CDS             complement(14170..14601)
                     /locus_tag="VGQ51_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEV9038217.1"
                     /translation="MSIWFDPSITPEKIRPLAHQTMVEHIGIEFTEVGDDYLKARMPV
                     DHRTRQPYGLLHGGASVSLAETLGSMASALVIDRQQYYCVGLEINANHVRGVREGWVT
                     GIARPLHIGKTTHVWDIRIHDASDKLVCVSRLTVAIVANLR"
     gene            complement(14601..15086)
                     /gene="coaD"
                     /locus_tag="VGQ51_16400"
     CDS             complement(14601..15086)
                     /gene="coaD"
                     /locus_tag="VGQ51_16400"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV9038218.1"
                     /translation="MNRIALFPGTFDPITIGHQDIINRSLPLFDKLIIGIGRNVNKEP
                     MFTEDQRLQWVRDIYKDNPKVDAVIYEGLTIKCCQQVGANFILRGIRYVNDFEYEKAI
                     ADMNRSLDHDIETIFLTCLPQYTSVASTLVRDVLKNGGDVMQFLPDVVARSLSKRQTT
                     R"
     gene            complement(15140..16084)
                     /locus_tag="VGQ51_16405"
     CDS             complement(15140..16084)
                     /locus_tag="VGQ51_16405"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV9038219.1"
                     /translation="MQLSTKHLLGIRDLQPGDIQLILDTATQFKEVLQRPIKKVPTLR
                     DVTIVNLFYENSTRTRISFELAEKRLSADTINFTASGSSAAKGETLLDTVNNILSMKV
                     DMVVMRHSASGAPHFLAKHISAAIVNAGDGINEHPTQALLDAFSIREKLGGLQGKKVA
                     IIGDIMHSRVALSNIHLLKKMGAEVTIAGPPTLIPVHIREAFDVRVEYDLRKTLEWCD
                     VANVLRIQLERQNQPLFSSLREYSLAYGISKRLLDSLDKEILIMHPGPINRGVELDSD
                     AADSKQSIILHQVENGVAVRMAVLYLLAGGTARGGSIR"
     gene            complement(16190..16711)
                     /gene="pyrR"
                     /locus_tag="VGQ51_16410"
     CDS             complement(16190..16711)
                     /gene="pyrR"
                     /locus_tag="VGQ51_16410"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV9038220.1"
                     /translation="MKIILNEQQLEITIQRLTHQLLENHLNLENTVLIGIQPRGIYFS
                     DRIVQQLTPLVPAGAIQYGKLDITFYRDDVRTGLHQLNQTDIPFSIENKNVILIDDVL
                     WTGRTIRAALDALLTFGRPEKVELCVLIDRRFSRQLPIQADYVGKSIDSIITQKVKVY
                     WVEKDGKDEVALL"
     gene            complement(16773..17321)
                     /locus_tag="VGQ51_16415"
     CDS             complement(16773..17321)
                     /locus_tag="VGQ51_16415"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV9038221.1"
                     /translation="MKKHLLASIVLLFSLVCANAQMTQGTFTFGAGLHLGLPVGDFHT
                     SASFGIGAELQGEYAFTENVTAVATTGYTYFAGKSFDDGFGDTYKVNYGHIPVLVGVR
                     YYATEQIFFGAQIGYGHYSVSVSGTNASGSSGTGGFEYRPQVGYNANPVQFVLSYDGT
                     SVTGGTFSQIGLSAIYNFGGGK"
BASE COUNT         3959 a         4385 c         5180 g         3900 t
ORIGIN      
        1 gcagaatacg ggccggattt cgagcatcag atcctgcgcg accggaatca tccgtccgta
       61 ttcatgtggt ccatcggcaa tgaagaatgg atgatacaga catcgactac gggaaagctg
      121 atcgcccaga cgcagataca gcggcagcgg ctgctggatc ccacccgtac ctgtacttat
      181 gcagccaacg tcggcaacgt ataccatggc gtcaacgagg tgataccggt gaggggattc
      241 aactataacc ttggcggtct ggatgggtat cgacgggatc atcccgacca gccgatcatg
      301 ggcaccgagg tggccagcac cgtctccacg cgcagcatct ttgaaaaaga taccgtgaac
      361 gcctatgtgc cggactacga cagcgtctat cctccgtggg cgtctactgc cgaacaatgg
      421 tggcgtattg cggccgatcg cccgtggttt atgggcggtt tcgcatggac aggcttcgac
      481 taccggggcg aaccgacgcc ttttggctgg ccggatatca attcgcattt cggtatcatg
      541 gacatgtgcg ggttcccgaa gaccgtgtat tactattatc aaagctggtg gaccgataag
      601 gatgtgcttc atatcgcacc acattggaac tggcccggca ccatgacagg acacgagata
      661 ccggtctggg taaactccaa tgccgataat gtggagcttt ttctcaacgg gaagagcctg
      721 ggtaaaaaag atatgccgcg caacggtcat ctggaatgga tggtgagtta tgccccgggt
      781 aaactggagg cggtggcata taagaaaggc cggaagctta cggccgtggt agagacgacg
      841 ggtgaaccgt atcgcatcgt ggtgaaggcg tcgaaaccgg ccctcggcaa tgagcccgaa
      901 gccgcgatcg tatacaattg cagcgtcgtc gatcgacagg gtagggaggt gcccgacgca
      961 tctaacctgt tgcatttctc ggtgaccggg aatgtacgta tcatcggcgt cggcaacggt
     1021 aatcccagca gtcatgagcc ggatcaattc aaggagggtg gatggcagcg acatttattc
     1081 gggggcagat gccaggtgat catcgaatcc gctggtcctg catgggatgc ggaccgggcg
     1141 tcattgatcg tttcggcgca agggctgcct tcggaaaaga tgtatatgca atagatgaaa
     1201 ctaccgcggc ctatagcaaa acagttcaag gtagccgcct acagttcacc ggccatcagc
     1261 gcattgaccg tcacgcccct gttcctcctg aggaatatcc ctttcgagct gtatccctgg
     1321 actttcggga tcgcagcgct gctggtattc attttctgga ccgtcaatat cggcatctat
     1381 cggatggtct cccgggacgc ccgcgatcgc aaccgggtca ggtatctggc cagctatgcg
     1441 ttttgcatcc tgttctcttt ttttcttttt cacgatcttt ttcaggaaca cttcggatcg
     1501 tcgcgcagtg gtcttcattt tcatgtgatc atcttttttg cagtggatac cgtcatcctg
     1561 atactccagg acctggtcgt caccagggag aagaatgtac ggattgaact cgagaattcg
     1621 cggctgcaca tgcgcgatat ggaggccatg aacctcaagc tcgctcagca ggtgcaccct
     1681 catttcctct tcaactcgct gagcaccctg aaagcgctga tctccgtcgc cccggagcag
     1741 gccaccgaat acctggtccg gctatcggga tttttgcgat cttcgatgac ggacaaagga
     1801 gtggtacggg tgggaaggga gctggaattg tgcggcgatt acctggcaat gcagcagatc
     1861 cgtttcggag aggccttacg atattcgatc gaggtcccgg aggaagtacg gggagcctat
     1921 ttcgtacccg tgttctcggt gcagctattg atcgaaaacg ccataaagca taacgtgctc
     1981 accccggacc gtcctttgtc aatcgatatc gtttaccggg atggcgtcat taccgtgaac
     2041 aataacctgc aacgaaaggc ggcgggttcc gctggtaatg cgagcggcct gatcaatctg
     2101 cgggaaagat accaggcact cggtggcgat ccggtcagga tagaagagac gaccgatcta
     2161 ttttctgtca gtataaagct gcttatccat gaatacggtg atcattgagg acgagccttt
     2221 gatggcgcag gacctgaaag catgtatcct ggcgtccgag ccatctgccc gggtggtcgc
     2281 tatcttatct tccgttcgcg aagcgatcgg gtacttccgc gagcaacctc ctccagatct
     2341 tattttcagc gacatacaat tgggggatgg tctgagtttt tccatcttcg cggccaacga
     2401 acaaaccatt ccggtgatct tttgtacggc ctatgacgaa tatgccctgg acgcgttcag
     2461 ggccgcgggg atcgattata tcttaaaacc gtactcttcg aaggacattg ccgcggcact
     2521 ggcaaaatac cggacattca ggggagaagc gccgtcgtat gacgagatcg cgcggtcttt
     2581 tggaaaacgc aaacagcaaa gcgtattggt ctattacaag gagaagatcg ttccggtgga
     2641 tgtcggggac atcgcgctgt tctatctgca taacgatctc acccggctgc tcacgttcgg
     2701 caaacagcaa tatgtcgtga gccggtcgct cgaagaatta gagcggacca ttgcgggccg
     2761 gttctaccgt gtaaaccgcc agttcctggt caaccggagg gccataatag acgtgtcgca
     2821 atatttcggg cgaaagctgt tgctcaatct ctctattccc ttcgatgaaa aggtgacagt
     2881 cggcaggctc aggaccaaca gcttcctgga atggctggca ggcgaatagg gcaattcgac
     2941 ccccgatttg ggtggttggg ctggaaatcg ctggcgccgt gatgggcggg cagttatctt
     3001 gtcgtcaaaa atcataacaa tgaagacaaa actgatcctt ctggcggcag ccggtctgct
     3061 gaccgcgggc attgtcatcc accattcgca tcgttgtccc ctgatgagcg ccaaggcggc
     3121 gcttacacag catcgctgag agaccagccg gccgcccgcc gaaggcgggc ggctttattt
     3181 cagccgataa gccgtcaccg tcaggccgtt gaatcccggc acccaaagcg tgcggttaga
     3241 tggatcatag cagatatcgg cggtattctt gtgcagtgcg tgtgtgtcga gcagcatttc
     3301 cttccggcca tctgcataga cataataaat atagcccacc cattcgctga cgagcagatc
     3361 gccgttgccg acctcttcga caccgtctcc accattgggc agactggtga tggtgcgcag
     3421 atgtttaccc gcatcggcga agagtacctg tttattcgcg aggatataca gtccgttttt
     3481 ggtcgccttg agcccgttag ctccatcgag atggtccatg tacaaggcgg gaacgtcttt
     3541 ttcgatacgg tagaccttgc cggctttcga atcggagaca tatactactc cggacccgtc
     3601 cacagttata tcgttgagcc caatggcacc ggcgatcgcg atctttttga tgatgcgccc
     3661 tttcggaata tccatgacca ccacgttgct gacgtcggct acataaagac ggttgccaaa
     3721 tcgtcccagg ccttttggag catcgagacc cgtgatccag gtggagtcat aatgctttcc
     3781 gtccagaccc aacttgccga ctgcgccttt gccgtcgaca tcccaggggc cgccttcgat
     3841 gagcgagacg aaaagaaagg aatgggtcgc atccggcagg acggattccg ggacattgac
     3901 gatgggagag gtctcccaga tcttagcgag cgaatgggca ttcgtcgaag tttgtgcgca
     3961 aagcgccagc ggggtgctga gagcgagaga caagattagc tttttcatat ccggtttaca
     4021 ttttagtgcc atgtgtttca tcggctgtcg gcggccagtc tttgttctgc aggtgtgccc
     4081 cgagcatgat cgttttgata tgccggacaa tgtccggatg ctgtgctgcg atattttcct
     4141 tttctccggg gtctttcgac agatcgtata gctcgatggg cggatcggga tcggtattca
     4201 cgccaagccg gacgcctttc caatttcccc agcggaccgc ctggcggccg ccgttctcat
     4261 gaaattccca atagaaataa tcgtgctgac gagccagacg gtccatgcct ttgagcagcg
     4321 gcaggatcga atagccgtcg atgtgctttg aagcgggcag accagccgtt tcctcgaacg
     4381 tgggaaaaag gtcccaaaac gccgccggcc ggttgcaaac gcttccggac ctgatcgtcc
     4441 ccggccacag ggcgataaaa ggttcacgga tgccgccttc atacagatcg cgtttgatac
     4501 cgcggaaggg tccgctgcca tcaaaaaatg cagggtctcc gccgccttcg cggtgcggac
     4561 cgttatcgct cgtgaagagg atcagggtct taccgcctat accgagttcc ttcacctcgc
     4621 gcaggatatc gccgacataa cggtcgagac ggctgaccat ggcggcaaaa gcggcatgcg
     4681 gataaggttc gaaggtatag tcggatcgtt tgtcggccgc acctggagct cgcggggatt
     4741 caccgaactg cgccacgtag tgtcgatata cgctgtcatg cggcacctgg agcgccgcgt
     4801 gagggagggt ataagcgagg tagagaaaga aagggcgatc gtggttttcg cgcaggaacc
     4861 gcagggcgtg ttgatggatc aggtcgccgg aatagaccga gtcctttttc agattgcccg
     4921 gaagctccag cttttcatca ttgatccaga gatggtcggg gtaatagtta tgcgccagcg
     4981 tctgacagtt gtaaccgaag aaatcggtga atcccttttt ccccggagta ccggtagagc
     5041 ccggatagcc cagaccccat ttgccaaagg cgccggtgat atatccgtgt tgctgaagga
     5101 cggtagcgat ggtaagcgaa gaatcgggga gggggtattg cccttccggc ttgaatccgc
     5161 gattgccgcg gatgggagta tggccggtgt gaagacctgt catcaggctt gccctggatg
     5221 gcgcacatac cgccgtgccg gcataaaact ctgtaaaacg catcccttcc ccggccatac
     5281 ggtcgatgtt gggagtgtga attttttgct ggccctggta gcctacgtcg ccatagccga
     5341 ggtcgtcggc gaggatgaga atgatattgg gacgcgtctg agcccggagg ataccggagc
     5401 aggagaaaag caggaagcag gcaaggtatc ttatcatgac ccgacaaaga aaagaaaagt
     5461 ttggccgatc ggacaaaccg ccgtacattt atattataat gatatcattt tgatatcaac
     5521 taaatttatc gttatggaac ctcttttctc cgagaagata caaaagccat cgagtggcta
     5581 tttcatgctc ttcgttatac tggccatatt ggtcttcgcc attctcctgt tggcccagcc
     5641 tggttcggcg ggtgcgggag tggtgtgcat ggtcctgttc atcctgctat ccaaagggtt
     5701 gctggtcgta cagcccaaca ccgcgaaggt cttcctttta ttcggcgcct accggggcag
     5761 cgttcgcacc agcggcttat actgggtgaa ccccttctat ggccggcaga ccctgtcgat
     5821 aagggcccgc aatttcgaaa gcgatcagct gaaggtcaac gacaagctgg ggaacccggt
     5881 attgatcagc atcatcctcg tctggaacgt gaaggacact tatcgggcgt cgttcgcggt
     5941 agacgattat gcaaagttcg tcaaagtaca ggcggatgcc gcggtgcgca agctggctgc
     6001 gtcgtatccc tatgaccatt tcgaggacga acgggcgggg ataaccctga gcacggattt
     6061 cgaccaggtg aatgccacgc tggagcacga gacggccgag cggctcgata tggccgggat
     6121 cgaagtggta gaggcacgga tcacccattt gtcctatgct ccggagatcg ccagcgcgat
     6181 gttgcgaaga cagcaggcta ccgccgtggt cgcggcacgt cataagatcg tcgaaggcgc
     6241 ggtgagcatg gtagagagcg ctttgaacct cctctcctcc aagaatatca tcgagctgga
     6301 tgaagaaaaa agggcggcaa tggtcagtaa cctcatggtc gtactttgcg gcgacagaga
     6361 agcccagcct atcgtgaata cggggacctt aaatcactga tgatggccga aaaaaagcct
     6421 tttgccctgc gaatgaacgc ggagatgatg cgtgccctcg agcggtgggc ggcggatgaa
     6481 ttccgcagcg taaacgggca gatcgaatgg gtgttgcaca aggcattacg ggaggcgggg
     6541 cgaatgaaag acaaaccggc gggcgagaag tcgccgaagg aaaaagaatg atcggcgtcg
     6601 tgtgggcgtg gtgaccgcat ctgggggcgc cgtgaccgca cctgggggca cagtcattgc
     6661 atgggttgga gaaaatgtct tactatgcca acgaaacaaa cgatcaaagc agcacaacgc
     6721 gacaaacgcc aggggaaatc cgcaagcaca caagccggtg aattcgtaaa agccgaaatg
     6781 gataggatcc gtaagggtca aaccggtgcc cggtcgtcga agcaagctat cgctatcggt
     6841 ctgtccgaag cgcgccgcgc cggtgtggag ctgaaagcgc ccgcaaaggg aaagacatcc
     6901 gagaagacga gaaagaacgc cgaaagagca ttggcgaagg gacagcggaa cgaaccgatc
     6961 gatcccgtcc ggtcgcgcgc gcggacaaaa gccatgaaga aagaaggtac cgaagccgcg
     7021 tcgccacggg cgttatcccg ccaggccact acggccgcca aacaacgtag cagtgcggat
     7081 cgcagtaatt cggccaaaca agcggcgcgc acgaaaggac ctgctgagcg cagcgcggcc
     7141 gcgaaaaaag cggcgcgtac gcgtgcacag cattcgcgat aaggccatat ctttgcgccg
     7201 ctgcgagtcg cggatatggc ggtgctgcaa cataggcgga tgctgaaata ccctgttacg
     7261 atactctttc tgctcctgac ggtcgctcag accttcagta agtggtgttt gatcgccgaa
     7321 ttcaggatca acagggacta tatcgcccaa aatctttgcg tcaaccgtaa caaaccttcc
     7381 tgttgctgca agggaaaatg ttatctgaac aagaaattgg cggaggatca atcgcagcag
     7441 cagacgccgg gtaaggacgc ccggcacgaa gaaatcccgc tgcaggtgtt gcgggctacg
     7501 aatatcgttc ctgtgcctgt ggtaacgata cttcatcatc gacctgcgac ccgtcatctc
     7561 gatgacgagc cgcaggaata tatcccttct tttttcgcgc cgccccggga gacgatgttc
     7621 tttgcttaag agaaacattg tcttcacatc aaatatccat tcatgtatgt ttttgtaagg
     7681 ggtctgttgt gcatgggcct gttgttcgtt ttccatacgg gcatcgctca aaccatcgta
     7741 aaaggaaagg tcatcgacaa catgacgggc gagccgcttg aaaatgcggt gatatcggcg
     7801 gggggaaaag ggagatcggt gctgaccgac gaacagggga acttcgagat cgaggtcgcc
     7861 gtgtccgtcg attctatccg ggcttctttt gtgggttatc gccggcagag ccaggagatc
     7921 gatcgcgggg acgagtcttg tctgttccgg ctatcccgga gtaccgtgga tatgcgatcg
     7981 atcacggtcc agccaatgcc caacaatgcc ttattttcag tcgtcagcca catagacgtc
     8041 aatctgcatg ccctgaattc ggcgcaggac ctgatgcgtc tcgtaccggg tctttcgctg
     8101 atgcaacacc agggcggggg catcgcggac catatctttt tccggggatt cgatgccgac
     8161 catggcaccg atgtcggtgt ttccgtagac ggcatgccgt tgaacatggt ttcgcatacg
     8221 cacggtcagg gtttctcgga tctgcacttc ctgatccccg agctgacgac cagcctggaa
     8281 tacgggaagg gaccctatta ttccgatcat ggtgatttca ccactgccgg ctatcttgcc
     8341 tttcggaccg tcgatgtgcc agagcgcaat gaggtgaaga tcgaagcggg aggcttccac
     8401 accggaagga tcatgaccgt cgtcaatctg ctcggcgctc aggccagaca gaggggaacg
     8461 agcgcctata tcgccgggga aggattttat acggacgggc cattcgatta tgcacaacac
     8521 ttcaaccggg ggaatctatt tggcaaatat ctacgggatc tgtctccgcg aacacaactc
     8581 aaggtaaccc tgtctacgtt cagcagtctc tggcgtagtt cgggcgagat ccccgaccgt
     8641 gcggtaaacg aaggactgat cagccgctgg ggttttatcg acagcactca gggggggaat
     8701 accgcgcgca gcaccgcaat cgtgcgtctg accacgcgcg tttccgacag gctcagctgg
     8761 gagaaccagg cctattttac ccactactca ttcggacttt gttacgatca gacatttttt
     8821 gcagacgata gtctcaatgg tgatcatctt ctgcagaagg agtccaggaa tatctacggg
     8881 tacgacggga agctatcgct tcacagttat ttgcccggcg gcggcgttct gtcctcatct
     8941 gccggattgg gatctcagtg gaacgatatc cgcggcagcg ggctcagcca tacgaatgcg
     9001 gcgcatgggg tattgaatga tctccaattt ggaaatatcc gcgagtggac cctcaatggg
     9061 tatatcgacg aacaataccg gaccgtgcat tggttattcg acgcgggcgc gcggttggat
     9121 tttctggatt tctattatga cgaccggctg aacccggtgc aaccttccct gggcagggcc
     9181 atcgtcagtc cgaaattcaa tatggaatat acgttcaacg accgctcaca ggtatacctg
     9241 cgtctgggta agggatttca ttccaatgac gccaaagtgg tggcggagga tcggggtatc
     9301 ggcgtcctgc ctgcggctta tggggccgat ctgggcatca actggaaacc tttcccgcgt
     9361 gtctatgtaa atgccggcat ctggtatctt gcactggagc aggagctgac ctatgacggg
     9421 gatgagggaa ccttttctcc gggagacaga acgagaaggg aagggatcga tctctccgca
     9481 cgctatcagc tgacgagctg gctttatgcc aatgccgata tgatcttttg caaagcgaga
     9541 gacgcggagg cggcgaaggg agagaattat ttgcctttgt ccgttccctt ttatgggtcg
     9601 gcgggtctgt atgtgaagct gccccatggc ttcagcggcg gatggaatgc ccgttatatg
     9661 cgggaccgtc cggccaatga agataacacg ctggtagcgc agggttattt cctgaacgat
     9721 ctgacggcca attatacaac gcgccggtgg gagatcggac tggagatcca gaacctgttc
     9781 aacgcaaaat ggaaggacgc gcaatatgag gtagaatccc ggttgaagac ggagccacag
     9841 gcggtggacg atatcagcta tacgccgggg atgccctttt ttgcgaaagt gaagctggcg
     9901 atattctttt aagaaaaaaa ccggcacaag ccggtttttt ttcgctagtt ctttttggca
     9961 tgaccgccgc cgtcttccgg tttcttgcaa caggtgggga gacgtttgta ggagtccggt
    10021 tcggctgtga cgtcatcggc gtcatagccc acattcgcga tggcggtctt gatattctcg
    10081 atattcgtgc gatcggtcat ccaggtgacc gtggttgttt tctttttata gtctacgttg
    10141 accttcacga tcccgtcttc gtgtttcatg taggtctcga tacggtcctt gcattgctcg
    10201 cattgtaccg tgggggtctt gatgacggcc gtcatctggg gtttggtctg ggcggaggcc
    10261 gtggtcgcga caccgatgag gaggacgagg aggaatttga atcttttcat atgtaatgga
    10321 atttgtccct taaagataag gaatttttgg gttaggcccc cgtccaggcg ccggggtcgg
    10381 cgctccattt cagcaatatg ctttcgtcct gcgggcttat gaggcctttt tccagcgcca
    10441 gccggatgag ggtcggataa tccgacagcg atataaaggg ggtccctgat ctttcaaaag
    10501 cctcgcgtgc ggtgtcgaat ccataattga aaatggatac caggccaacg acctgcagac
    10561 cttcctggcg gaggatatcg accacctgca ggctgctctt gccggtagag atgaggtctt
    10621 ccacgacgac gacgggttga ccgggctggt aatagccttc gatctgttgt cccagcccat
    10681 gctctttcgg cttgctacgg acatagacat aaggcagctt gagctggtct gcggccatgg
    10741 cgccccaggg gatgccggcc gtggcgacgc cggcgatcag acccgcatcg gggaattggg
    10801 tgaaaatgac attacataat tcggatttga cgaaatcccg gatatagggg aagccaagga
    10861 ccttccggtt atcgcaataa atggggcttt tccagccaga cgaccaggtg aagggctctt
    10921 ttggactgag acggatagcc tgggcctgta acagccgctc ggcaacggct cgttcattgg
    10981 tggtgatgat cttttctccc ataatgcgaa agtaggtaaa ttgtagtatg tacgtcaaaa
    11041 tctacttttc agacaaaccc cttttcctgt gcgatgaaat gacccccgag atcgtcgctt
    11101 actcgcatca tgacgacgcg gtgctgatcg atgagttcga tcaccatacc gtgaatacca
    11161 tgctgcatga gatgcgacag ccgaaagtgc atgcggggat ctttatccac aaagacttcg
    11221 aagtcctacg caaggctttt ttcaaaaaat tcctattggt gaaagcgggg ggcgggctgg
    11281 tgaggaatgg cgatggaaaa tacctgttca tgctccgtcg gggcatttgg gatctgccta
    11341 agggcaagct cgacccggga gagacgatcg agcagtgcgc cgttcgcgag gtggaggaag
    11401 agacggggtt gcaaaaggtt gcgctgcaga gtccgctgct cgtcacctat cacacctatg
    11461 acgaaagtgg aaagcatatc ctgaaggaga cgcattggtt tcggatggtc gtcgcagacg
    11521 ggtcctcgct gaagccgcag accgaagagc agatcaccga attgcgatgg gtagccgatg
    11581 gggagatgaa gccattgctg caacgtacgt tcccgtcggt ggcggacgtc attcggtccg
    11641 caaactcctg acggggttgg tcagggcggc ctttatcgtt tgaaaactga ccgtcagcag
    11701 ggtgatcagg agcgcgccgg ccgcggggag aacgaaacac caccaggtca tggcggtgcg
    11761 gttgggataa tgacgcagcc aggtatgcat gcctaaccat gcgagcggga tggcgatgac
    11821 caacgataaa aagacgagca tcacaaattc tcctgacaag agttgccaga gattgaatac
    11881 ggaggcgccc aataccttgc gcacgccgat ctcttttgtg cgttgttcgg ccatataggc
    11941 agacatcccg aaaagtccca ataaggaaat aaagatggca agacaggcaa agacgccggc
    12001 cagttgaccg atgcgttgtt cgttctcata attgcgggca tattcggcat cgacaaactg
    12061 gaactggaac ggctcatcgg gattgtactt cgtgaagatg gcgccgacag cggccaatga
    12121 ccgagccacg cttctccccg gagtgagacg aacgataaag gcgctgttta catcggtatt
    12181 aagggtgaat acgaccgggc gcacctcttc ataaggggac cccatgacca ggtccttaac
    12241 gacaccgatg atcctgtaag gtatattttc cgaggtgatg atcgtgccga ccggctgttt
    12301 cagccccatc aggcggacgg ccgcctcgtt caatataaag gccgacgtat cgctggagaa
    12361 atcgcgcgag aaatcccgac cggctgcaag gtcccaacct atggtcttac cgtagtccca
    12421 ggaaatgttc ataaaggcga tgtctgcaaa ataatttggg tccttaccgg gccaggatat
    12481 gccgctggtg ctgctgtagg tattgacggg cgatccatca ccttctgcga tggaaacgat
    12541 cgctctttgt ccgataagct ctttttgtac ggcgtcgaaa tgcttatgga tatttttcgt
    12601 cgacaccgtg atgctgatga gaccgtcacg gtcataacct gcggggcggt ccttcgcgta
    12661 ctggacctgg aggtatacga cgatggtgcc gatgataagg ataacggaga ccgcaaattg
    12721 caatacgacc aggaccttgc gcgggataga agcgttccgt cctacgcgga aatttccttt
    12781 tagtacgttg actggatgga aagaagaaag atacaaagcc ggatagctac ctgcgacgag
    12841 accggtagtg atcgtgaaga ccagtgcggt cagccagaac caggcattgt tccagggtat
    12901 agacatgcgt ttgtctgcaa tctcattgaa aaaaggcagg ctgatcccga ccagtacgag
    12961 cgaaaaagca aaggcaaaga tggccacgag cagcgattcg ctgaagaact gaccgatcag
    13021 ttgttgccgt cgcgagccaa tggctttacg gatgcctact tcgcgggcgc gtccttcgct
    13081 gcgtgccgtc gaaagattca taaaattgat acaggccagc agcaggacga agatgccgat
    13141 gatgccgaaa agccagatgt actggatccg gcctccggtg ttcaccccat ccctgaactc
    13201 cgaatagaga tgccatttgc tcatggggaa aaggaagagt ttcggtttcg aggcggccag
    13261 tcttttgtta agcattttaa gcttggcgtc acggatgttt tgggagacct tgtcgaatgc
    13321 agccccatcg gtcagctgga catagagttc gaaggcattg ggccgccagg ggtcctggat
    13381 ggtctttatc catgacatat tggtgtaata gagctgccag ccgccgatga attgcagatc
    13441 gcgaaaggtg gaagagtagg gaaggtccgc atagatgccg gctaccttgc cattaaaact
    13501 attgtcgagc cgtatcgttt gtcccatagg gtcactatcg ccgaacagcg cttttgcggc
    13561 ggaggcggag aggaacatgg aagacgggtc attgagctca accccccgac cctttagcat
    13621 tttcagatca agaaggctgg tgaccggggc ttcataaaaa ccccctttta gattcatttg
    13681 ttttaagccg acaccgagga tatggttatt ggcgccgatg cccatgacta cactggtgaa
    13741 atctgctccg taatgcttcc gaagctcgtc ggcgagcgga tagggtaccg tgttccaggt
    13801 ggacacgctg ccgttatggg taatattctg gataacgcgg gcgacccgat ggtaatggga
    13861 atcctgggta tcgaagctgt attcgtctgc gatccacaac ccgataagga tggcgacggc
    13921 catgcctacg gaaaggccgg aaatattgat aagggaatgg gccttgttct taatgagatt
    13981 gcgccaggca accaaccagt aattcttgag catggagatg aaggattgcg gaaaggggaa
    14041 ctaaaaggat gccgggttgc aagatgttga tggtcaggtt gttgtgaaga gggtgttggg
    14101 gtaggtgtac ggaaatgata cgggggatgt ccggcgacgg tcatcgcagg cccgctacga
    14161 tggcaagggt catctaaggt tcgccacgat ggcgaccgtg agacggctga cgcagaccag
    14221 cttgtcgctc gcgtcatgga tacggatatc ccagacatgt gtggtcttgc cgatgtgaag
    14281 cggtcgtgcg ataccggtaa cccacccttc gcggacccca cggacatggt tggcattgat
    14341 ctccagaccc acgcaatagt attgttgtct atcgatcacc agcgcggagg ccatgcttcc
    14401 cagcgtctct gccagcgaga cggaggcgcc accatggagc agcccatagg gctgacgtgt
    14461 ccggtgatcg accggcatgc gtgccttcag atagtcatcc ccgacttcgg tgaattcgat
    14521 gccgatatgt tccaccatgg tttgatgggc cagtggccga atcttttccg gcgttatgga
    14581 agggtcgaac cagatggaca tcagcgggtc gtttgtcgtt tggataatga gcgggcgacc
    14641 acatcgggga ggaactgcat cacgtccccg ccgttcttca atacgtcccg caccagcgtg
    14701 gaggctacgg aggtatattg cggcagacag gtgaggaaga tggtctcgat atcgtgatcg
    14761 agactgcggt tcatatccgc gatggccttt tcgtattcga aatcgttgac gtaacggatg
    14821 cccctcagga tgaagttggc gccgacctgc tgacagcatt tgatggtaag accttcatag
    14881 atcaccgcat ccaccttggg gttatccttg tagatatccc tgacccattg gagtcgttgg
    14941 tcttcggtga acatgggctc tttgttgaca ttgcgcccga tcccgatgat gagcttgtcg
    15001 aacagcggca ggctgcggtt gatgatgtcc tggtgcccga tggtgatcgg atcgaaggtt
    15061 cccgggaaga gggcgattcg gttcatagac aacggcaatt taggataaaa aaccgatacg
    15121 gctggccgtg gcgactggtt tatcggatgg aaccgccgcg tgctgtgcct ccggccagga
    15181 gatacagcac ggccatgcgt acggctaccc cgttctccac ctggtgaagg atgatcgatt
    15241 gcttactgtc cgccgcgtcg ctgtccagct cgaccccccg gttgatggga ccggggtgca
    15301 tgatcaggat ctctttgtcc aggctgtcca ataatcgttt gctgatacca taggcgaggc
    15361 tgtattcccg cagcgaggag aacagcggct ggttctgacg ttcgagctgg atacgtaaga
    15421 cgttggcgac atcgcaccac tcgagggtct tccggagatc gtattccacg cggacgtcga
    15481 aggcttcgcg gatatggacg gggatgagcg tgggggggcc ggcgatggtc acctcggcgc
    15541 ccattttctt cagcaggtgg atattgctca gcgccacgcg ggagtgcatg atgtcgccga
    15601 tgatggccac cttcttacct tgcagaccgc ccagcttttc ccggatggag aaggcgtcga
    15661 gcagggcctg ggtcgggtgt tcattgatgc cgtcgcccgc gttgacgatg gcggcggaaa
    15721 tatgtttggc gaggaagtgc ggggcgccgc tggcgctgtg tcgcattacc accatgtcca
    15781 ctttcatgga caggatgttg ttgaccgtat ccagcagcgt ttcacctttg gcggcggaag
    15841 agccggaggc cgtgaaattg atggtgtccg ccgacaggcg cttttccgcg agttcgaagg
    15901 agatgcgggt acgggtagag ttctcgtaaa agagattgac gatggtgaca tcccggaggg
    15961 taggcacctt tttgatgggt cgctgtaaaa cttctttgaa ttgggtcgcc gtgtctaaaa
    16021 tgagttggat atcgcccggc tggaggtctc taatgcccag aagatgtttg gtgctcagtt
    16081 gcattagaac gcaaaattaa gcgatttttt gaatttttca taaatccgcc ggagatagtc
    16141 gcctggagag gctgtgggca gaggccgccg ggagagaccg tgggggccct cataaaagtg
    16201 cgacctcgtc cttaccatcc ttttccaccc aatagacctt gaccttttgg gtgataatgg
    16261 aatcgatgga tttgccgacg tagtcggcct ggatgggcag ctggcgggag aaacggcgat
    16321 cgatgagtac gcatagctct actttctccg ggcgtccgaa ggtcagcagg gcgtcgagtg
    16381 cggcgcggat ggtacggccg gtccagagca cgtcgtcgat aaggatgacg ttcttgtttt
    16441 cgatagaaaa ggggatatcc gtctggttca gctggtggag gccggtacgg acatcatcgc
    16501 ggtaaaaggt gatatccagc ttgccatatt ggatggcgcc ggcggggacg agcggggtca
    16561 gctgctgaac gatgcggtcc gagaaataga tgcctctcgg ctggatgccg atgaggacgg
    16621 tgttttcgag gttgagatgg ttttcgagca gctggtgtgt gagccgttgg atggtgatct
    16681 ccagctgctg ttcgttcagg atgatcttca ttgtataaaa gtaaataaaa agccggcgcc
    16741 cggggcgccg gctcggaagt ttgccagcaa aattatttgc cgccgccgaa gttatagatc
    16801 gcggagaggc cgatctggct gaaggtaccg ccggtgacgg aggtgccatc atagctaaga
    16861 acaaattgaa ccgggttggc gttgtagcct acctgggggc ggtattcgaa gccgccggtg
    16921 ccgctactgc cactggcgtt ggtgccagag acactcacgg agtaatgacc atagccgatc
    16981 tgggcgccga agaagatctg ttcagtggcg taatagcgga cgccaacgag caccgggata
    17041 tgcccgtaat tgaccttgta cgtgtcaccg aagccgtcat caaaggattt gccggcgaag
    17101 taggtgtagc cggtggttgc gacggcggtg acattctcgg tgaaggcgta ttcaccctga
    17161 agttcggcgc cgatcccgaa ggatgcagaa gtgtgaaagt cgccgacggg caggccaaga
    17221 tggagaccag cgccgaaggt gaaggtgccc tgggtcattt gcgcgtttgc gcagaccagg
    17281 gagaaaagga ggacaataga agccaggaga tgctttttca tacttatata ggtttgtacg
    17341 caaatctaat aggcaaattt gaaaaaacaa agtggccgcc cgaaagagcg gccactttgc
    17401 aaacattcta tacaaacttc aata
//
LOCUS       DASYSV010000525         5151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1261504, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000525 DASYSV010000000
VERSION     DASYSV010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261504"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VGQ51_16420"
     CDS             <1..372
                     /locus_tag="VGQ51_16420"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEV9038222.1"
                     /translation="TVSMGVLLGLVIGKPLGIFLVSRLMVALNLAHLPSNTNWKQLLG
                     MGTLAGIGFTMSIFTTMLAFKEESFRDIARISVLLAVVVSTGISIVYFRAIGGKYAVK
                     PMNVPKTQQPHPQADLGLNAA"
     gene            complement(450..3032)
                     /locus_tag="VGQ51_16425"
     CDS             complement(450..3032)
                     /locus_tag="VGQ51_16425"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV9038223.1"
                     /translation="MNYQPSQGLQQFKNLVGIKFQLYNSLFTSLPFHRIEKTGILLSL
                     LLTICEDGYKKKQSPEQILEDFFQKHTAFSGEREKADILFRFVQYVERQIVLFDALED
                     AAFKHVNDMGGPGTLKHLISEVLQTDKEPELTEKLKDYSVRLVLTAHPTQFYPGAVLG
                     IINDLSRVVIENNAADINLYMQQLGKTPFLKKQQPTPFDEAISLIWYLENVFYHASGR
                     IISALKEQFPHAVDPKNAVIRMGFWPGGDRDGNPFVKVETTLRVAEALRAAIVRCYYQ
                     DVRRLKRRLTFKGVDVMIADLEQTLYNNILAPEGRTDLSPAGLLDQLNQIREIIIYQH
                     NSLFVGLVDNLIAKVMIFGLHFATLDIRQDSSVHENVLAAIGARQGLLPEDYASLGPA
                     AKMEALMALKGGAQAELYEDPLVRDTLETVNAMRTIQRLNGEEGCCRWIISHSSSALN
                     VLEVMGLFLLGGWKKKDLPVDIIPLFETVDDLRDAASVMKELYEHTDYKAHLKQRGNT
                     QTIMLGFSDGTKDGGYLMANWAIYKAKEELTRVSKQYGIDVVFFDGRGGPPARGGGKT
                     HRFYASMGRNISNKEIQLTVQGQTVSSNFGTIDTAQFNIEQLIHAGISNDLFSEKEVT
                     LQAGEEAMMQELASESYKAYSTLKNHPYFLQYLNEVSPLRFYSETNIASRPSRRKSSG
                     RLELKDLRAIPYVGAWSQLKQNVPGYYGVGRALQSMDKKGKWKEVKQLYMDSPFFKTL
                     LDNCEMAMKKCFFPLTEHFSTHPVYGEIWALIYDEFELTQRYIFKLSGKNELMADYPV
                     EQLSIQMRERIVLPLSTIQQYALSKFQALEKGGGGGRPMKEVYEKLIMRSSFGIINAA
                     RNSA"
     gene            3318..>5151
                     /gene="thrA"
                     /locus_tag="VGQ51_16430"
     CDS             3318..>5151
                     /gene="thrA"
                     /locus_tag="VGQ51_16430"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HEV9038224.1"
                     /translation="MQVLKFGGSSVANSENISKVVTLVLDAIKKDRTILVVSALGGIT
                     DMLIQAATSAATGDESYKDRLRQIEHRHLETVKSLLPITGQSAILSAVKTRCNEIEDI
                     CSGVFLLGELSARTQDKIVSYGELLSSLIVSARFDALGTKHEWKDSRQLIRTDSHFGH
                     AAVDFNKTNSLIRDAIAVSPANLFIFPGFIASNDQGITTTLGRGGSDYSAAILAAAVD
                     AQVLEIWTDVSGMMTADPRLVSSPKLIPHISYQEAMELSHFGAKVIYPPTIQPVMRKA
                     IPVWIKNTFSPADTGTVIEVETQRNGSSIRGISSIPRIALLSLEGSGMVGIPGFSKRL
                     FEALSGQQVNVILITQGSSEHSICVGIDDANAAIAKSAVDQAFEHEIGIGTVEPLVVE
                     RDLSIIALVGDRMKSHPGVSGKLFGALGRNGVNVRAIAQGSSERNISAVIAAGDVRKA
                     INVLHEEFFETTYKQLNLFITGTGNVGRRLLDQLLQQQAWLLQQLRIQLHVVGLANSR
                     KMVFRDEGIDLRQWKQQLENGQPMQLDRWVDGITSRNLRNAVFVDVTANSDVALQYRT
                     LLQKSISVVACNKIACSSAYGSYRQLKDLAREYNARFLFETNVGA"
BASE COUNT         1208 a         1667 c         1108 g         1168 t
ORIGIN      
        1 acggtcagca tgggcgttct tctgggtctc gtgatcggta aaccgctcgg catcttcctg
       61 gtcagccgcc tgatggtggc cctcaacctg gcccatcttc cttccaatac taattggaaa
      121 caactgctcg gcatgggcac cctggccggc atcggcttta ccatgtccat ttttaccacg
      181 atgctggctt tcaaagaaga aagctttcgg gacatcgcca ggatctccgt cctgctcgcg
      241 gtggtggtct ccacggggat cagcatcgtt tatttcaggg ccattggcgg gaaatatgcg
      301 gtcaagccga tgaatgtacc aaaaacgcag caaccacatc cgcaggccga cctcggcctc
      361 aatgctgcgt aagactatac ccgcacagat tgacccccgc ctggcccatc ggcaatcacc
      421 tcactacctc ccggctctgc ctacccgcct taagccgaat tcctcgccgc attgatgatc
      481 ccaaaactac tccgcatgat cagcttctca tacacctcct tcatcggcct tccaccacct
      541 ccccccttct ccaacgcctg gaacttcgag agcgcatact gctgtatcgt actcaacggc
      601 aatacgatcc tctccctcat ctggatcgac aactgctcca ccgggtaatc ggccatcagc
      661 tcattcttac ccgacaactt gaaaatatac cgttgcgtca gctcgaactc atcatagatc
      721 aatgcccata tctccccata taccggatgc gtggagaaat gctccgtcaa cggaaaaaaa
      781 catttcttca tcgccatctc acagttgtcc agcaaggtct tgaagaacgg cgagtccata
      841 tacagctgct tcacttcctt ccacttgccc ttcttatcca tcgactgcaa agccctgcct
      901 accccataat aacccggtac gttctgcttc agctggctcc acgcgccgac ataagggatt
      961 gccctcaggt ccttcaactc gagccgcccc gaacttttcc ggcgggaagg ccggctcgcg
     1021 atattcgtct cactataaaa acgcaatgga ctcacttcat tcaaatactg caagaaataa
     1081 ggatgattct tcaatgtact gtaagcctta taactttccg aagccagctc ctgcatcatc
     1141 gcttcctcac cggcctgcaa cgtcacctcc ttctccgaga acaggtcatt ggatatcccc
     1201 gcatggatca gctgctcgat gttgaactgc gcagtgtcga tcgtcccgaa attcgaagac
     1261 accgtctgcc cctgcacggt caattgtatc tccttattgg aaatattcct tcccatcgac
     1321 gcatagaaac gatgcgtctt gccgcccccc cgcgcaggcg gtccgcccct gccgtcgaaa
     1381 aacactacat cgatgccata ttgcttcgat acccgggtca attcttcctt ggccttgtag
     1441 atcgcccagt tggccatcag gtaccctcca tccttcgtcc catccgaaaa tcccagcatg
     1501 atcgtctggg tattcccccg ctgcttcaaa tgcgccttgt aatcggtatg ctcatacagt
     1561 tccttcatca ccgacgccgc atcccgcaga tcgtctaccg tctcgaataa gggaataatg
     1621 tcaaccggca ggtctttttt cttccagcca cccagcaaaa acaatcccat cacctcaagc
     1681 acattcaacg cactgctgct atggctgatg atccaccggc agcaaccctc ttcgccatta
     1741 agacgctgaa tggtccgcat ggcattgacg gtctccagcg tgtccctgac caacggatcc
     1801 tcatacagct ccgcctgcgc cccacccttc aatgccatca aggcttccat cttcgctgcc
     1861 ggccccagag aggcatagtc ttcgggaagc agcccctgac gcgcaccaat agccgccagc
     1921 acattctcat gaacgctgct gtcctgccgg atgtccaggg tggcgaagtg aagcccgaag
     1981 atcatcacct tggcgatcag gttatccacc aggcccacga acaggctatt gtgctggtaa
     2041 atgatgatct ccctgatctg gttcagctga tccagcaacc cggcaggcga aagatccgtc
     2101 cttccttccg gcgccaatat gttattatac aacgtctgct cgagatccgc aatcataaca
     2161 tccacccctt tgaaggtaag ccggcgcttg agacgccgaa catcctggta ataacaccgg
     2221 acgatcgcag ccctcaacgc ttcagccaca cgtaaggtcg tctccacttt cacgaaagga
     2281 ttcccatccc ggtcaccccc cggccaaaat cccatccgga tcacggcatt cttaggatcg
     2341 acagcgtgcg ggaactgctc cttcagcgcg gaaatgatcc ttccgctcgc atgataaaat
     2401 acgttctcca aataccagat cagactgatc gcttcgtcaa atggagtagg ttgttgtttc
     2461 ttcaggaagg gcgtcttccc caattgctgc atgtaaaggt tgatgtccgc cgcattgttc
     2521 tcgatcacca cccgcgacag gtcattgatg atccccagca ccgccccggg atagaattgc
     2581 gtcggatgcg ccgtcaatac cagccttacc gaataatcct tcagcttctc cgtcaactcc
     2641 ggctccttat ccgtctggag cacttccgat atcagatgct tcaacgtccc cggcccgccc
     2701 atgtcattca catgcttgaa agcggcatcc tccaacgcat cgaaaagaac gatctgacgc
     2761 tccacatact gaacgaaccg gaataaaata tccgctttct cccgctcccc cgaaaacgcc
     2821 gtatgcttct gaaaaaaatc ttccagtatc tgctccggcg actgcttctt cttatatcca
     2881 tcctcacaga tcgtcaataa caatgacaac agtatccccg ttttctctat ccgatgaaat
     2941 ggcagggagg taaaaagact attgtaaagt tgaaatttga tacccaccag gttcttgaat
     3001 tgctgcaacc cctgggacgg ttgataattc ataatatcaa tttaacactg ttcaaagaat
     3061 gcggtaaaaa cgcggccttc attcaaaaca acctcaaatt tactttattt caactaagaa
     3121 atcggcaatt ttttgttttt acattcactt tttgtctatc ttagcggccg tgatcatctt
     3181 cagtcaccat acaggcctct ctctcccggt tgttgaaatt acaactatta tctccattac
     3241 aacccgttaa gggttgtcga ttaccatatt accaccgggg ctcatccaag ctgttctcat
     3301 ccaaaatcac tcattgtatg caggttttaa aattcggcgg ctcttccgta gccaactcag
     3361 aaaatatctc caaggtcgtt acactcgttc tcgacgccat caaaaaagac aggaccattc
     3421 tcgtggtttc cgcgctcggc ggcataacgg atatgctcat ccaggctgcc acatccgcag
     3481 cgacaggcga cgagtcatac aaagatcggc tacggcagat cgaacatcgt cacctcgaaa
     3541 ccgtgaagtc cctcctgccg atcaccggcc agagcgccat cctcagcgcc gtcaaaaccc
     3601 gttgcaatga aatagaagat atctgcagcg gcgtcttcct gctcggcgaa ctctcggccc
     3661 gcacacagga taagatcgtc agctatggcg aactgctgtc ctcactcatc gtctccgcac
     3721 gcttcgacgc tctcggcacc aaacacgaat ggaaagattc ccgccagctg atccgcaccg
     3781 attcacattt cggacacgcc gccgtcgatt tcaataaaac caactccctc atccgcgatg
     3841 ctatcgccgt ctctcccgcc aatttattca tcttccccgg gttcatcgcc tccaacgatc
     3901 agggcatcac gactacgctt ggaagaggcg gctcggacta ttccgccgcg atcctggcgg
     3961 ctgccgtcga cgcacaggtg ctcgagatct ggacagacgt ttccggcatg atgaccgccg
     4021 atccccggct cgtttccagc cccaaactca ttccgcatat ctcataccag gaggctatgg
     4081 agctctcgca tttcggcgcc aaggtcatat accctcctac catccagccc gtgatgcgca
     4141 aggccattcc cgtctggatc aagaatacct tctcccccgc agataccggt acggtcatcg
     4201 aggtcgaaac ccaacgcaac ggcagcagca tccgcggcat ctccagcatc ccccggatcg
     4261 ccctgctcag cctcgaaggc agcggaatgg tcggcatccc cggcttctcc aaaagactgt
     4321 tcgaagccct ctcgggccaa caggtcaatg tcatcctgat cacccagggc tcttccgaac
     4381 attccatctg cgtcggcatc gacgatgcca atgcagccat cgccaagtct gcggtcgacc
     4441 aggccttcga acatgagatc ggtatcggta cggttgaacc actcgtcgtg gaacgcgatc
     4501 tgtcgatcat cgcactggta ggcgatcgca tgaaatcaca tcccggcgtc agcggcaaac
     4561 tattcggcgc cctcggccgc aacggggtca acgtccgcgc tatcgcgcaa ggctcctccg
     4621 aaaggaatat ctccgccgtc attgcggcgg gtgacgtaag aaaggcgatc aacgtcctcc
     4681 acgaagaatt cttcgaaacc acctataaac aactcaacct cttcatcacg ggtaccggca
     4741 atgtaggccg ccgcctgctc gaccagctcc tgcagcaaca ggcctggctg ctccagcaac
     4801 tgcgcatcca gcttcacgtg gtaggcctgg ccaacagccg caaaatggtc ttccgcgatg
     4861 aaggcatcga cctccggcag tggaaacaac aactggaaaa cggtcagccc atgcagctgg
     4921 atcgatgggt cgatggcatc acctcccgca atctgcgcaa tgccgtcttc gtcgacgtca
     4981 cggccaattc ggatgtcgct ttacaataca gaacgctgct ccagaaaagc atctccgtcg
     5041 ttgcctgcaa taagatcgca tgttcctcgg cgtatggcag ctaccggcag ctgaaggacc
     5101 tcgcccgcga atacaacgcc cgcttccttt tcgaaacgaa tgtaggagca g
//
LOCUS       DASYSV010000526         8911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1134228, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000526 DASYSV010000000
VERSION     DASYSV010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134228"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2041
                     /gene="tkt"
                     /locus_tag="VGQ51_16435"
     CDS             <1..2041
                     /gene="tkt"
                     /locus_tag="VGQ51_16435"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV9038225.1"
                     /translation="TTTMANPPAATKDIEQLCIDTVRVLSADAVQKAESGHPGTPMAT
                     APMGHVLWAKHMRYNPRDPAWPNRDRFVLSAGHACMLQYSFLYLTGYDLSLEDIKKFR
                     QLHSKTPGHPEYGLTPGIEVTTGPLGQGFANGIGMAIAQKHLAARYNKPGFDLFNYTI
                     YAICSDGDMMEGISSEAASIAGHLGLGNIVYLYDDNHITIEGDTAITFNEDVGLRFEA
                     YGWHVQSVEDGNDIGALDRAVRAAREEKSRPSLIRVRTHIAYGSPHKVDTAAAHGSPL
                     GADEVRLVKEFFGFDPDESFVVPGKVLEYYRSIGEKGAAPEQEWLSLFESYKKEFPDL
                     ASEYELALQDRLAQGWEKKIPTFRAAEGKEGKMSTRVAAGKTLNAIAGLLPCLLGGAA
                     DLAPSTETHLKDYSDFEPGDFGGRNFHFGVREHSMGAILNGMAVTKGLIPYGATFLIF
                     SDYMRPPVRLAAIMKIRPIFIYTHDSIGLGEDGTTHQPVEQLAGLRSIPNMTVIRPAD
                     ANEAAQAWKAALLHMGGPVALILTRQNIAIIETGNYKTTGDVSKGAYILSEPDRAPEL
                     ILIASGSEVGLILQAQLKLKENGIAARVVSMPSWELFEKQDDGYKQRIFPKGLRRRLA
                     VEAASPQGWHKYVTDEGDMLGMTRFGESAPASDLFREFGFTVENVVARAKYLLGR"
     gene            complement(2050..3114)
                     /locus_tag="VGQ51_16440"
     CDS             complement(2050..3114)
                     /locus_tag="VGQ51_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9038226.1"
                     /translation="MVKVLVLEHDIMDVELLTYELKKNRFEFELEVAQTKEEYAAALV
                     RFGPGIILADYNLPSFDAEQAFRMKQERLPDTPFIIVSGAIGEENAVALIKKGVTDFV
                     PKDKLFTIVPKMNRALQETKERRERLEAERRLEEERMRHQRMLTRAAIEGQEKERATI
                     GKELHDNINQMLSIVKIYISLAEEGGDDFRQLLKQSDGMINKCINEIRGISHMLIPPP
                     LKEEGLIPAVDTLIERIKIAKPFSIYFNHQEHRGKEIDEEEQLAIYRIIQEQFNNIIK
                     HAHATRVDVELTEDDEQVFLRITDNGQGFDTRKKRAGVGLNNMLSRVKVFDGDLQIIS
                     GRGAGCTLKVSLPKEHEMAH"
     gene            complement(3133..4998)
                     /locus_tag="VGQ51_16445"
     CDS             complement(3133..4998)
                     /locus_tag="VGQ51_16445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV9038227.1"
                     /translation="MAAAKKATILIVDENTQNIRSLERLLSARDRVFVFAANGKDALK
                     TALSKDLDLILLEVSMPSMDGAEVAQILKTNRRTRDVPIIFISSSPVASGAEEGAIDY
                     LYRPLDPAITRARVSALLQQYSQKKELAEKDTALENYALLIGNSTDLLCTLDADTLKF
                     EDVNAAVTDVLGYTVEEIKGSSLLFYLHEEEKQKVQSLTKENSERFNFETRVWTKNRA
                     IRLLHWRVVRRDGRWFAGARDVTGTKEAEEIKHYLATVVKQSDEAIYLHDADGRIISW
                     NRGAERIYGFTEKEALKMKIWNIVPEHLMAETQQLVNTVIAGAEVQSVETTRITRQGR
                     VIDVRLSASVLLDAANKLLSIAITERDITEKKRAEEEIRQANRELENALSDLKLVNRE
                     LESFSYSVSHDLRSPLRAMDGYSQMLTDEFHEFLNEEGRRLLGNIRRNATRMGTLIDD
                     LLEFSRLGRKELHKSPVDMGELVGEVLQVMEASVGRSGAVIAVRELPPAHADRSLLRQ
                     VWTNLISNALKYSAKTQSPEITIGFIGRGGAGECGYFVQDNGVGFNMEYAHKLFGVFQ
                     RLHSHEEFEGTGVGLANVQRIISRHGGRVWAKGEPGKGATFYFSIPGVGDGGEGT"
     gene            complement(5022..5834)
                     /locus_tag="VGQ51_16450"
     CDS             complement(5022..5834)
                     /locus_tag="VGQ51_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEV9038228.1"
                     /translation="MTDRTALKGLLDSIRLVYGYDFTEYSEASLGRRVAHFMKNRDIP
                     GIRELARSVLRDEGFFEQFVQEVTVTVTEMFRAPGFYKSLREKVRARLATYPMIRVWI
                     AGCATGEEVYSMAILLREEGLLERTVIYATDINQRSLQTAREGIFPIGHMAAYTANYL
                     QSGGKRSFSDYYLAKYDGALMDKTLRQAIVFSAHNLTTDQSFNEFQLILCRNVLIYFN
                     QNLQNKVIRLFYESLCPFGILALGNKESLLFSDKGDKYKTMDKKERIFIKQP"
     gene            complement(5838..6242)
                     /locus_tag="VGQ51_16455"
     CDS             complement(5838..6242)
                     /locus_tag="VGQ51_16455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9038229.1"
                     /translation="MITIEDKLKGRSILIVDDDDRNLYALSCYLEAMVPGMTVYRAPG
                     GQEAICILEKHQDIALVLMDIMMSRLDGYETIKRIRSCDKLKHLPVIAVTALVLDGDR
                     DRCLLAGASDYVSKPIDTEQLLKSIAGALNLV"
     gene            complement(6247..>8911)
                     /locus_tag="VGQ51_16460"
     CDS             complement(6247..>8911)
                     /locus_tag="VGQ51_16460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9038230.1"
                     /translation="KQKIEIDAITDAIYKSNLTVEFDLNGKITKANDNFLDLLGYTAA
                     DLIGRHHSVLVEKGVEKTEAYSAFWQALRRGEFVQGEFKRMTRNGEPVWIRGNYNPIM
                     DAGGRPYKILKIATDVTLARLQAQELAAQTEELASQTEELETQQEELKQLNEEMRDKN
                     LLLEHSQAQLESQQEELRQTNEELEEKAGLLEAQKQRLEVAKLEVETKARELEISGKY
                     KSEFLANMSHELRTPLNSILILAQLLTDNRSGVLGEKEVEYSKNIRSSGTDLLNLINE
                     ILDLSKVEAGRMELEITDIDLGSLTEKLIAQFHEVAKHQGIHFEVNLGKEAAEHGLST
                     DRQRLEQILKNLLSNAFKFTEKNGNVSLDIRFLPERGQTKLPGQPATVSFSVRDTGIG
                     IPENKREVIFNAFQQADGSTKRKYGGTGLGLSISRELAHALGGEIGMESEEGKGSVFT
                     LSLPARTDRSAVAAIPKETKTTKRIARGSGEKAGPNIADDREAIGDTDKVVLIVEDDA
                     DFARILLDISRQRGCKGIVARQGNAGLSLARFYKPDAILLDMKLPVMDGADVLRQLKN
                     DPGLRHIPVQILSGDDRRKEGLDLGAFDFIQKPVSLADLQRAFARIEEFADKKLKKLL
                     IVEDNQQQNKAIRELVGNGDVQTVECFTGTEAFAFLTQQSFDCVIIDLGLPDMSGMQL
                     LEKIRDNEELRRTPVIVYTARDLSREEASRLNKLASTVILKTADSRERLLDETTLFLH
                     RMESRLPREKQQIIRRLHRMDEVLKNKKVLIVDDDMRNIYSLTNVLEEEGMQCITAEN
                     GQVAVKLFRENPDIDMILMDVMMPGMDGYEATKEIRRLPTGLRIPVIALTAKAMKGDR
                     EKCLEAGMSDYISKPVNIEQLLSLMRVWLYK"
BASE COUNT         1780 a         2666 c         2261 g         2204 t
ORIGIN      
        1 tacaaccact atggccaacc ctccggccgc aacaaaggat atcgaacaat tgtgtatcga
       61 tacggtaagg gtcctgtccg cggacgcagt acaaaaggct gaatccgggc accccgggac
      121 tccgatggcc acggcgccga tgggtcatgt gctatgggcg aaacacatgc gttacaatcc
      181 cagggatccg gcatggccga atcgcgaccg gttcgtactc tcggcgggtc atgcctgcat
      241 gctccaatat agttttctct acctgacggg ctatgatctt tcgctcgaag atatcaaaaa
      301 attccgccag ctgcacagca agacacccgg acatcccgaa tacggactca cgcccggtat
      361 cgaggttacg acgggccccc tgggacaagg gtttgccaac ggcatcggga tggccatcgc
      421 gcagaaacat cttgccgccc ggtataataa gcccggtttc gatctgttca actataccat
      481 ctatgccatc tgcagcgatg gcgatatgat ggaaggcatc agttccgaag ccgcctccat
      541 tgccggtcat ctcggtttgg gcaatatcgt ctatctctat gacgacaacc atatcactat
      601 cgagggggat acggctatca cgttcaatga agatgtcgga cttcgttttg aggcctatgg
      661 ctggcatgtg cagtcggtgg aagacgggaa tgacattggc gccctggacc gggccgtcag
      721 ggccgcgcgt gaggaaaaat cgcgtccttc gctgatcagg gtacgtacgc atattgccta
      781 tgggagtccg cataaggtgg atactgcagc ggcccatggc tctccgctag gtgcggatga
      841 ggtccggctc gtaaaggagt ttttcgggtt cgatccggac gaatcgttcg tcgtgccggg
      901 gaaagtgctg gagtactacc ggtccatcgg ggaaaaaggc gcggcgcccg aacaggagtg
      961 gctatcgctg ttcgaatcgt ataaaaagga atttcccgat ctggcgtccg aatacgaatt
     1021 ggcgctgcag gaccggcttg cgcaagggtg ggaaaaaaag atccccacct tccgggcggc
     1081 ggagggaaaa gaagggaaaa tgtcaacccg ggtagcagcg ggtaagacgc tgaatgccat
     1141 tgcaggcttg cttccctgtc ttctcggcgg cgccgcggac ctcgcgccca gtacggagac
     1201 gcatctcaag gactattcgg atttcgaacc cggggatttc ggcggacgca atttccattt
     1261 cggcgtccgt gaacattcga tgggcgctat cctcaatggg atggcggtca cgaagggttt
     1321 gatcccttat ggcgccacct tcctcatctt ttcggattat atgcgtcctc ccgtccggct
     1381 ggccgcgatc atgaagataa gacccatctt tatctatacg catgacagta tcggactcgg
     1441 tgaagatggt actacgcatc agccggttga acaattggcg ggtcttcgca gcattccgaa
     1501 tatgaccgtc attcgtcccg ccgatgccaa tgaggcggcg caggcctgga aagcggcgct
     1561 gctgcacatg ggcggtccgg tggcgcttat ccttacccgt cagaacatcg ccatcatcga
     1621 gaccggcaat tacaaaacca ccggcgatgt cagcaagggt gcgtatatcc tgtcggagcc
     1681 ggacagggcg ccggagctga tcctcatcgc ctccggatcg gaagtcgggc tgatcctgca
     1741 ggcacagctc aagctgaaag agaacggcat cgcggcccgg gtggtgagta tgccttcctg
     1801 ggaattgttc gagaaacagg atgacggcta caaacaacgg atattcccca aagggttgag
     1861 gaggagactg gcggtcgagg cggcctcgcc ccagggctgg cacaaatacg tcaccgatga
     1921 aggcgatatg ctgggaatga cgcggttcgg cgagtctgcg ccggcttccg atcttttcag
     1981 ggaattcggg ttcacggtcg agaatgtggt ggcgcgcgcc aagtatttgt tgggaagata
     2041 ggcgtggcct caatgcgcca tctcatgctc tttcggaagt gacaccttta gcgtacaccc
     2101 cgctccgcgg ccggaaatga tctgaaggtc tccgtcgaat accttcaccc ggctgagcat
     2161 attattcaat cccacccctg cgcgtttttt tctcgtatcg aaaccctgcc cgttatcggt
     2221 tatccgcagg aacacctgct catcgtcctc cgtcagctcg acatccacac gggtggcatg
     2281 tgcatgtttg atgatattgt tgaattgctc ctggatgatc cggtagatag ccagttgctc
     2341 ctcctcgtct atttcctttc cgcggtgctc ctggtgattg aagtagattg aaaaaggctt
     2401 tgcgatcttt atccgctcga tcagcgtatc caccgccggg ataagacctt cttctttcag
     2461 cggcggcggt atcagcatgt gcgaaatgcc cctgatctcg ttgatacatt tgttgatcat
     2521 cccatcgctc tgcttcagca gctggcgaaa atcatcgcct ccttcttccg ccagcgagat
     2581 atagatcttg acgatggaaa gcatctggtt gatattgtca tgcagctctt ttccgatcgt
     2641 ggctctttcc ttctcctgcc cctctatcgc ggcgcgggtc agcatcctct gatgacgcat
     2701 ccgctcttct tccagccgtc gttcggcctc cagcctctcc cgccgctcct tcgtctcctg
     2761 gagggcccgg ttcatcttcg gcacgatggt gaacaatttg tccttgggaa cgaagtcggt
     2821 gactcccttt ttgatcagcg caaccgcgtt ttcctcgccg atagcacccg atacgatgat
     2881 gaacggcgtg tccggcagcc gctcctgttt catccggaaa gcctgctctg catcgaatga
     2941 cggaaggtta tagtcggcca ggatgatccc gggtccgaac cgcacgaggg ccgcggcata
     3001 ttcctccttc gtctgcgcca cttcgagctc aaattcgaaa cgattctttt tcaactcgta
     3061 ggtgagcagt tcgacatcca tgatatcgtg ctcgagcact aaaactttta ccatagtccg
     3121 cgcttttaga ttttatgttc cttccccgcc gtccccgact ccgggtattg aaaagtaaaa
     3181 ggtcgcaccc ttccccggct cccctttggc ccatactctc ccgccatgcc gggagatgat
     3241 ccgttggaca ttggcaagcc cgacacccgt accctcgaat tcctcatgac tgtgcagccg
     3301 ttgaaaaacc ccgaacagct tatgggcata ttccatattg aaaccaactc cattgtcctg
     3361 cacgaaataa ccacattcgc cggcaccgcc ccgcccgata aacccgatag tgatctccgg
     3421 cgattgcgtc ttcgccgaat acttcagcgc attcgagatc aggttggtcc acacctggcg
     3481 aagtaacgac cggtccgcat gggccggcgg taattcccga accgcgatca cggcgcccga
     3541 acgcccaacg gaagcctcca tcacctgcag cacctcgcca accagttctc ccatgtccac
     3601 gggcgatttg tgcaattcct ttctccccag ccgggagaat tccagcagat cgtcgatcag
     3661 cgttcccatc cgcgtggcat tgcggcggat atttcccagc agtctcctgc cttcctcatt
     3721 caggaattcg tggaattcgt cggtgagcat ttgcgaatag ccgtccatcg ccctgagcgg
     3781 agatcgaaga tcgtgcgaga cggaataaga gaaggactcc agttccctgt tcaccagctt
     3841 cagatcggaa agtgcatttt ccagctccct gttggcctgt ctgatctcct cctccgcccg
     3901 ctttttttcc gtgatatctc tttccgtgat cgcgatggac agcagcttgt tcgccgcatc
     3961 cagcagcacg gatgcggata accgcacgtc gatgaccctt ccctgcctgg tgatccgcgt
     4021 cgtttccacc gactggactt ccgcgcccgc gatcacggtg ttcaccagtt gttgcgtctc
     4081 tgccatcagg tgctccggca cgatattcca gatcttcatc ttcaatgcct ccttttccgt
     4141 gaacccgtat atcctttcgg cgcccctgtt ccaggatatg atccggccat cggcatcatg
     4201 gagatagata gcctcatccg actgtttaac gacggtagcg aggtaatgct ttatctcctc
     4261 ggcctccttg gtgcccgtga cgtcccgggc gccggcgaac cagcggccat cgcgccggac
     4321 gacacgccag tgcagcaatc gtatggccct gttctttgtc cagaccctcg tttcaaagtt
     4381 gaatctctcg ctgttctctt ttgtaaggga ctggaccttc tgcttttctt cctcatgcag
     4441 atagaacagc agcgagctgc cctttatttc ttcgacggta tagccgagca cgtcggttac
     4501 cgccgcattc acatcctcga atttcagcgt gtcggcatcg agggtacaaa gaagatcggt
     4561 cgaattgccg atcagcaggg catagttttc aagggccgta tccttttccg ccagttcctt
     4621 cttttgcgaa tattgctgca acagcgcgga gaccctggcc cgggtgatcg ccggatccag
     4681 tggccggtaa aggtagtcga tggcgccctc ttcggccccg gaggcgacag gcgaggacga
     4741 aataaaaatg atcggcacat cccgcgtccg ccggttggtt tttaatatct gcgccacctc
     4801 cgcaccatcc atcgacggca tcgagacctc gagcaggatg agatcgaggt ccttgcttag
     4861 cgcggtcttc agcgcatctt tcccgttggc ggcgaaaaca aaaacccggt cacgcgccga
     4921 caatagccgc tccagcgaac ggatattctg cgtattctca tcgacgataa ggatcgttgc
     4981 cttttttgct gccgccatat tcgcctcccc aaaattaccg atcagggctg tttgatgaat
     5041 attctttctt ttttgtccat tgttttatac ttgtctcctt tatcggagaa gagcagcgat
     5101 tccttattgc caagggccag gataccgaaa ggacaaaggc tttcataaaa taatcggatc
     5161 accttgtttt gcaggttttg attgaaatag atcagcacgt tccggcaaag gatcagctgg
     5221 aactcgttga acgactgatc cgtcgtcagg ttatgcgccg agaatacgat ggcttgccgg
     5281 agtgttttgt ccatcagcgc gccgtcatat ttggcgaggt aatagtcgga aaagctgcgc
     5341 tttccgccgc tttgaagata attggccgta tatgcggcca tatgaccgat cgggaagata
     5401 ccctcccttg ccgtttgaag actgcgctgg ttgatatccg tcgcatagat cacggtccgc
     5461 tccagtaaac cctcttccct taacaggatg gccatggaat agacctcttc accggtggcg
     5521 catcccgcga tccagaccct tatcatcggg taggtggcca gtctggccct taccttctcc
     5581 cgcagacttt tgtaaaagcc gggggcgcgg aacatttccg tcacggtcac cgtcacttcc
     5641 tgcacaaact gctcgaagaa cccttcgtcc cgcaagaccg atctggccag ctcacggatt
     5701 cccgggatat cccggttttt catgaaatga gctacccgcc gtccgagcga tgcttccgaa
     5761 tattcggtga aatcgtaccc gtacaccagc cggatggaat ccagcagacc tttcagggct
     5821 gttctgtctg tcatttctta cacgaggtta agcgctccgg ctatggattt cagcagctgc
     5881 tccgtgtcga tgggtttgga cacataatcc gaggctcccg ccagcagaca tctgtcccgg
     5941 tcgccgtcca ggaccagcgc cgtcaccgct atcaccggca aatgtttcag cttgtcgcaa
     6001 ctcctgatcc gtttgatggt ctcatacccg tccaaacgcg acatcatgat gtccatgagg
     6061 acaagagcta tgtcctggtg tttctccagg atacagatag cctcctgccc gcccggcgcc
     6121 ctataaacgg tcatccccgg caccatcgcc tccaggtagc aggacaacgc atacaaattg
     6181 cgatcgtcgt cgtcgacgat caggatactc ctccccttca gcttatcttc tatcgtgatc
     6241 atgcatttat ttataaagcc acacccgcat cagggataac agctgttcta tattcaccgg
     6301 tttggagata taatcggaca tccccgcctc caggcatttt tccctgtctc ccttcatagc
     6361 cttggcggta agggcgatca caggtatccg caaccccgtg ggcagccgcc tgatctcctt
     6421 tgtcgcctca tacccatcca tcccgggcat catcacatcc atcaggatca tgtcgatatc
     6481 cgggttctct ctgaacagct tcaccgccac ctggccgttc tcggccgtta tacactgcat
     6541 tccttcttct tccagcacat tggtcagcga atagatgttt cgcatatcgt catccacgat
     6601 caataccttt ttatttttca ggacctcgtc catccggtgc agtctccgga tgatctgctg
     6661 cttctcgcgg ggaagacgcg attccatgcg gtgcaggaag agggtcgttt catcaagcag
     6721 tctttcccgg gagtcggcgg ttttcaggat caccgtgctc gcgagcttat tgagccggct
     6781 ggcctcctcc ctggaaagat ctcttgccgt atagacgatg acgggcgtac gccggagctc
     6841 ttcattgtcc ctgatcttct ccagcaactg catcccgctc atgtccggca acccgagatc
     6901 gatgatgaca cagtcgaaag actgctgcgt gaggaaggca aaagcctccg tgccggtgaa
     6961 gcattcgacc gtttgtacat ccccgttacc gacgagctca cggatcgcct tgttctgctg
     7021 ttggttgtct tcgacgatga gcagcttttt cagtttctta tccgcgaatt cctcgatccg
     7081 ggcaaaggcg cgctgcaggt ccgccagcga caccggtttt tgaataaaat cgaaggcgcc
     7141 cagatcaagt ccctccttcc gacggtcgtc ccccgaaagg atctgcacgg ggatatgccg
     7201 gagacccgga tcgtttttca attgccggag gacatcggct ccgtccatga ccggcagctt
     7261 catgtccagc aggatcgcgt cgggtttata aaatctggcc aggctcagac ccgcattgcc
     7321 ttgccgggca acgatgccct tacatcccct ctgccgcgag atatccagca ggatcctggc
     7381 aaagtctgca tcgtcttcga cgataaggac gaccttatcg gtatccccga tggcttcccg
     7441 gtcgtcggcg atgttcggcc cggccttttc gcccgatcct ctcgctattc ttttcgttgt
     7501 cttagtctcc ttaggaatgg ccgccaccgc agaacgatcc gtccgcgccg ggagagatag
     7561 cgtaaagacg ctgcctttgc cttcttcgct ttccatcccg atctcgccac caagcgcatg
     7621 ggccagctcc ctactgatgg aaagcccgag ccctgtgcct ccatatttcc gtttggtaga
     7681 cccgtccgcc tgctgaaagg cattgaagat gacctcgcgt ttgttttcgg gaatgccgat
     7741 gcccgtatcc ctgacggaaa aactcacggt tgccggttgt cccggaagct ttgtttgacc
     7801 gcgttcgggc aaaaaccgga tatccaggct gacgttccca tttttctccg tgaacttgaa
     7861 agcattggaa agcagatttt tcaggatttg ctccaggcgt tgtctgtccg tggacagccc
     7921 gtgctccgcc gcctcttttc ccagattcac ctcaaaatga attccctgat gtttcgccac
     7981 ttcgtggaac tgagctatga gcttttcggt cagacttccc agatcgatgt ccgtgatctc
     8041 cagctccatc cttccggcct ccaccttgga gagatcgagg atctcattga tgaggttcag
     8101 gaggtccgtg ccgctgctgc ggatattttt cgaatattct acctcttttt ctcccagtac
     8161 cccgcttcta ttgtcggtga gcaattgtgc caggatgagg atactgttca gcggggtcct
     8221 cagctcatgc gacatattcg cgaggaattc ggatttgtat ttcccggaaa tttccagctc
     8281 ccgggccttc gtctccactt ccagcttggc cacctccagc cgttgtttct gcgcttccag
     8341 cagacccgcc ttctcttcca gctcctcatt cgtctgtctg agctcttcct gttgcgattc
     8401 gagctgcgcc tgtgaatgct ccagcaacag attcttatcc ctcatctctt cattcagctg
     8461 tttcagctct tcctgctgcg tctccagctc ctcggtctgt gaagccagct cctccgtctg
     8521 ggccgccagc tcctgcgcct gtaaccgcgc cagcgtgacg tccgtggcga tcttcagtat
     8581 cttgtacggc ctgcccccgg catccatgat aggattgtaa ttgcccctga tccatacggg
     8641 ctcgccattc ctggtcatcc gcttgaactc gccctgcacg aattctcctc tcctcagcgc
     8701 ctgccagaat gcgctatatg cctccgtctt ttcaaccccc ttttcaacca gcacgctatg
     8761 atgccggccg atcaggtccg cggcggtata gcccagtaag tcgagaaaat tgtcattggc
     8821 ctttgtgatc ttaccgttca gatcgaactc cacggtgagg ttcgacttat agatcgcatc
     8881 cgtgatggca tcgatctcta ttttctgttt t
//
LOCUS       DASYSV010000527        16219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2155511, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000527 DASYSV010000000
VERSION     DASYSV010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 16219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16219
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2155511"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..701)
                     /locus_tag="VGQ51_16465"
     CDS             complement(255..701)
                     /locus_tag="VGQ51_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038231.1"
                     /translation="MDLEKYAIFKGVDDFYYEFYSVGPNGRIKKIVTFTALTDYEDEY
                     YNLYLGDWLEAESRASDNSVSNNGDRDKVLATVASIIVHFTALMPKAVIFAQGSTPSR
                     TRLYQIGIKSHWQEINEDFDVHGFRQAKWERFQKSRNYEAFLLRRK"
     gene            1019..2302
                     /locus_tag="VGQ51_16470"
     CDS             1019..2302
                     /locus_tag="VGQ51_16470"
                     /inference="COORDINATES: protein motif:HMM:NF025021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEV9038232.1"
                     /translation="MSTVSRDFPAHPHLDVPKKQARELLSQVRARVTDALDRIRRRHP
                     HFQSTEDDTISIRLKLSDAQLVIAREYGFSSWTQLRKRITGNTAAQLIDKAIRANDAA
                     AVTQLLTAYPNLLDVPVVSGNWGPPMSHAANLGRLDMVKTIAALGAKDFQHAFDRALL
                     HGDIEIARWLHEQGAQFTPGIITGSCETLNERGFAFLVEAGVPLTDAKGDSLAPLAMI
                     LETYSRNPAGKHAILRLFRDRGYQWADTPIMAFHCGDLDRLTSHLQRDPQVIHRRWAY
                     REIYPPSLGCADDSRSGLHGTPIDGTTLLHLSIDFDERDIFDWLLEHGADVNAVATID
                     NEGFGRHTPLYNALVCQAYGAGRQRDSYMVRRLLESGADIHLRVDLRKYLDWREEPGW
                     HIARNVTPLEWAVNFPETGWVNREAVRMIENMRRE"
     gene            complement(2376..3416)
                     /locus_tag="VGQ51_16475"
     CDS             complement(2376..3416)
                     /locus_tag="VGQ51_16475"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HEV9038233.1"
                     /translation="MSVYVDKQKGKPDFSWDAGRLLRSAAESRYRQGRLSGRMSAMAA
                     SLRDEAFIGALTLEAVRSAELEGETLPPGQVRSTFAARLGLVSGVPAPAGIDAVSAAG
                     MILDVHLHYDQPLTEKRLTEWHRSIMPAVGRRSTGLVHYQAPAPHAGPAKLRAFLHWF
                     NADGDIDPVIKAGVAHWWFMTVRPFDGSNGRIARAITDLQLARAEEGGYRYYSLSEAI
                     VRERDVYARFFRNAPDARTAAASDITPFLEWFLHCLGQALTSDHLAAILRKASFWEGH
                     AAFEFNERQRLIIGRLLDDAETRLTSSRWARLTDTSSDTAVRDINDLVAKGVLVKESA
                     GGRSTSYVLAQG"
     gene            3537..4031
                     /locus_tag="VGQ51_16480"
     CDS             3537..4031
                     /locus_tag="VGQ51_16480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV9038234.1"
                     /translation="MITSTSNQLHWYSIISPNFAKAIRYALNTDFTGMDIGKYPIDGE
                     NVFAIVNEYTTKPVSGCDPESHRDYADIQIMVAGSERFGYAPLTDQPASTPYNPARDV
                     AFYSIAEDELNYITLGPGQFIIFFPSDIHQPEVFVSQPQLVRKVVLKVGLRPMADYSQ
                     TSVE"
     gene            4143..6098
                     /locus_tag="VGQ51_16485"
     CDS             4143..6098
                     /locus_tag="VGQ51_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEV9038235.1"
                     /translation="MRIFFALLALSICPAAFSQPSSGVPTPLPDLIPAPVSETINSGH
                     FILPHMVLIEAGDQQALARILEDLRMHLSVPTGYNVTVTRQSSRNATIRLILNTKPEA
                     SLGSEGYTLNITSKSILIRANQPAGLFYGVQTLFQLFPPAIESRLQVHRDSWEAPCVT
                     ITDYPRFGWRGLMLDVSRHFFSKQEVKDFIDEMVKYKFNLLHMHLTDDEGWRVEIKGL
                     PRLTQVGAWSAKRVGTFGTFPDPAPDEPRDYGGFYTADDIKELVKYAKDRFVNILPEV
                     DIPGHSLAAIVSYPELSCTPGADKYHVRSGEKIMNWYKGGFSAIYDNTLCPANEKVYP
                     FLDTVFTTIAKLFPFPYIHVGGDECAKNFWEQSDAVKQLMQRENLKNMEEVQSYFEKR
                     VEKIVESKGKKVIGWDEILEGGLAPNAAVMSWRGVKGGIAAAKMGHSVVMSPTTYFYL
                     DYMQGDASIEPPVYATLRLKTTYSYEPVPDSVDPKYILGGQANMWNEQVYNTRHLQYM
                     IWPRGMAVAEDLWSPKEKKNWDNFAARVEEGFRRLDVEQVKYSTSMFDAIITPKKDLT
                     AFDSITVDLATEVNGLDIYYSFDNSNPDNFYPKYTGTLTIPKDASWLKVVTYRNGKQI
                     GRQLNLTIGELQRRAGVAMPKPTPVAD"
     gene            6146..6856
                     /locus_tag="VGQ51_16490"
     CDS             6146..6856
                     /locus_tag="VGQ51_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038236.1"
                     /translation="MKLWGFLFPALLAATVSSFGQSRTDSSFSLLFNSGISFTHANDP
                     HINRWLEKYGYPPEPHIPTSINFEIAAVPAFSRLLYTVKLSSINSGSNLSSYNISAGL
                     YASLLKSKHFFLYLGGSVGLHGDIITLNGQLPPEYEQMAASYHAPLALRRRGLFVEPA
                     ARVFWYPIHFRAVQLGVFAALGYDVDINSKWKLGYYSNNHGKYSHFRALGKPGDQMKV
                     SEYGVFYIGGLSFRVNLH"
     gene            complement(6929..9589)
                     /locus_tag="VGQ51_16495"
     CDS             complement(6929..9589)
                     /locus_tag="VGQ51_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEV9038237.1"
                     /translation="MTAEHQRLAANAAKKIPLEQWGPYLSERQWGTVREDYSPYGDAW
                     HYFPYDHAHCRTYFWGEDGLGGISDFFQNLCFAVALWNGKDPILKERLFGLGNKEGNH
                     GEDVKELYYYLDNLPTHYYMEYLYKYPQQEFPYQQLLEENRKRSREEPEYEILDTGVF
                     DQNEYFDVLITYAKQTARDISIRIDIHNRYDKAAEITVLPTIWFYNRWQYDGLEKKPV
                     IASRDRHSVKANHGRLGAYYFYFQPPDDMLFTENETNHEKVSGKPNKTPFVKDAFHEA
                     IINGNDIEALRHKRSGTKFAPVYRYTIGGGETRTLYLRLSNRVLENPFGEDPNAVFQV
                     RKKEADAFYQSILPANITSDMAAVQRQALAGLLWSKQYYHFDVERWLTTSDGITPVNP
                     GKLNGRNHDWKHLKNQDIVAMPDKWEYPWYAAWDLAFQSIAMALVDPVFAKHQLMLIM
                     REWYMKPDGQIPAYEWNFSDVNPPVQAWAAMEVYRIEKERTGKGDIAFLKKIFQKLLI
                     NFTWWINRKDANGNNMFEGGFLGLDNIGVFNRSHALGGDMQLEQADGTSWMGMYALNM
                     MDMALEIAMKDDTFEDTATKFFEHFVLIAEALNEQGMWNDEDRFFYDTLSIGGSPVQL
                     RIQSIVGLTTLFAVSIIEKKMLHRLSDFKKRITWFESYRIKNKRYWPNEEHSDGEQIL
                     LSLVPQKRLIYLLERLLNEAEFLSPGGIRALSKYHEGNPYNINIGGVDYHIKYDPGDS
                     TSDFFGGNSNWRGPVWIPINYLIIRSIRQYGKFYGDGLMVEYPAGSGTKLTLEAVADH
                     LTDRVISLFRKDKSGGRPLHGKYNWFYQQPGNERLVLFYEYFHGDTSHGLGASHQTGW
                     TSLVAELISELSGRKIPSPPAREKTATIRE"
     gene            9746..11125
                     /locus_tag="VGQ51_16500"
     CDS             9746..11125
                     /locus_tag="VGQ51_16500"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV9038238.1"
                     /translation="MTFKEFGLDENLLEGIEASGYENATPVQEQVIPLILQGKDIIAS
                     AQTGTGKTAAFLLPIVNRLLASRHDDQVNALVIVPTRELAVQIAQNLEGLSYFTPISS
                     IAVYGGGDGSSFSTEKQALSRGADVVICTPGRMIAHLNMGYVRTQGLQYLVLDEADRM
                     LDMGFYDDIMKIISFLPHQRQNLLFSATMPVKIRDLARKILHDPAEVNIAISKPPARI
                     VQQAFVVYETQKIPLVKWLLKSSNLKSTLIFCSKKHNVKQLTMELRRGKFNVNEIHSD
                     LEQSAREDVLLEFRSRRLPILVATDILSRGIDIDNIEMVINYDVPNDGEDYVHRIGRT
                     ARAETDGVAVTFISEKEQNKFRGIEELLGQPVPKAAVPEQFGAAPEYNPGSRGKGGGR
                     GRGRGGPKGGHGGGKGTHPAGHSGGGQNKHGHGGQHGKTGHSGEGHGGHSAHRRKPSS
                     SGEQAPKQG"
     gene            complement(11112..12350)
                     /locus_tag="VGQ51_16505"
     CDS             complement(11112..12350)
                     /locus_tag="VGQ51_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV9038239.1"
                     /translation="MTVLFRHIAFLKAVLLHSISAFGGPQGHLGMMMKTFVHRRGDIT
                     EEELMDYSAFCQLLPGASSTQTLTLIGYKRGGVVLAMLTLIIWTLPASCLMALFSFFP
                     MRTTGLFRFIGPMTIGFLAFAAQQAFRISIRNTITRIILVVSTIATFLLFQWPWIFPI
                     LLVLGGFVTNLSSRRIPQVEVLPKKIKWGNIWLFAIIFLLAGASSELARKNNWPNRRP
                     LNVFENTYRFGSLVFGGGNVLMPMMYEQFVARPQSQKLIQRNPHIIRMNKEDFYSGWG
                     MVRAIPGPVFSVASYMGGIAMKDKGTTWQILGCFIGATGIFLPSALLVLFFFPIWHNL
                     KKYATVYRALEGINAVVVGVMVAAALYMMKDFRAASLLDIALNLVVITATWALLNYSR
                     LPSPVIVMIWMALGGALILV"
     gene            12457..13770
                     /locus_tag="VGQ51_16510"
     CDS             12457..13770
                     /locus_tag="VGQ51_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV9038240.1"
                     /translation="MKSPFSLLVLLLSCFSLASAQTNVSAAIDAAADKLEPQTIAWRR
                     DFHEHPELSNREFRTSKIIADYLRSLGLEVKEGVGKTGVVGVLKGDLPGPVVGLRADM
                     DALPLVERTPVPFASKVRTTYIGQDVGVMHACGHDSHMAILMSVAKILSDMRHQLKGT
                     VKFIFQPAEEGAPPGEEGGAALMIKEGALDNPKVDVLFGLHINAATEAGKIKYRSEGA
                     MAASDWFTIRVHGKGSHGSQPWGGIDPIVISAQIIEGLQTIISRQTELTRDAAVISVC
                     VINGGVRSNIIPEEVEMTGTIRTLDTGMQRDIHERMRRTVTRIAESAGATADISIDNK
                     TLITYNDPVLVKRMLPSLQRTAGEKNILWMDAMTGSEDFSFYGTKVPSFFFFLGGMRP
                     GQDPHTTAPHHTPDFYIDESGFKLGVKAFCHLVFDYADSVTLKLA"
     gene            13798..14157
                     /locus_tag="VGQ51_16515"
     CDS             13798..14157
                     /locus_tag="VGQ51_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV9038241.1"
                     /translation="MDVESIREYCLAKDAVEEGFPFGETTLVFKVQGAIFLLIGLDNP
                     ELQFNVKCDPEQAIEWREQYAAVQPGYHMNKRHWNTVIVDGTIPARILRQMIDDSYRL
                     VVQKLPKKLRPPGASHL"
     gene            complement(14148..14723)
                     /gene="nadD"
                     /locus_tag="VGQ51_16520"
     CDS             complement(14148..14723)
                     /gene="nadD"
                     /locus_tag="VGQ51_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEV9038242.1"
                     /translation="MKVGLYFGSFNPIHHGHLLIATYVLQHTNIQQVWFVVSPQNPLK
                     PTASLLNEYHRLHLVQVAIEGARDLRASDIEFRLPKPSFTVNTLAYLQEKYPVNEFSI
                     IMGSDSFQNLPKWKNGEWLLRNFPVYVYKRPGHLSLPLYEDAKEIHDLDAPLLPISST
                     DIRKNIRESKSIRYLVPDTVREEIERNGYYR"
     gene            complement(14720..14950)
                     /locus_tag="VGQ51_16525"
     CDS             complement(14720..14950)
                     /locus_tag="VGQ51_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEV9038243.1"
                     /translation="MRRLSELEPGTIARIHSFEKNDLFLKLMEMGCVPGELVRVEQIA
                     PLGDPISITVAGYNLSLRLDEAENIFMEEGDR"
     gene            14985..15419
                     /gene="ybeY"
                     /locus_tag="VGQ51_16530"
     CDS             14985..15419
                     /gene="ybeY"
                     /locus_tag="VGQ51_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV9038244.1"
                     /translation="MTIPTIQFNFLESIALRDRRKLKLFLVRLFKKEGRPLAELQYIF
                     CSDKYLLAINRQYLNHDDYTDIITFDLSEKNHPINAEIYISVDRLRENALKYDSSLKK
                     ELLRVMFHGALHLCGYKDKTATDQGEMRKMEEKYLRSYDRST"
     gene            15508..>16219
                     /locus_tag="VGQ51_16535"
     CDS             15508..>16219
                     /locus_tag="VGQ51_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9038245.1"
                     /translation="MFQEYDVIVVGAGHAGCEAAAAAANLGSSVLLITMNMQTIAQMS
                     CNPAMGGIAKGQIVREIDALGGYSGIVTDKSMIQFRMLNRSKGPAMWSPRAQNDRMLF
                     AATWREMLEKTPNVDFYQDMVSSLIIKEGRVCGVVTGLGHTISAKAIVVTSGTFLNGI
                     IHIGEKRFGGGRVAEKAATGITEQLVSEGFESNRLKTGTPPRIDGRSLDYSKMEEQRG
                     DEIITGFSYLDLPKPSEQR"
BASE COUNT         3911 a         4223 c         4272 g         3813 t
ORIGIN      
        1 caaataaaaa gccgaaatct atatttcggc tttcgaatta cattttataa aagagtgctt
       61 catttcttct tggataacga tttcggcact ccatgcttct tcaggaaagt aaccgctttc
      121 ttcgctttct gatgtatagc cggatcatta ccatgatctt gaatactctt gtcgataaca
      181 acttctatgt tcaacttctt attagcatta atgtttccca ttttcactaa gattatcgat
      241 tcaaatttac gaatttattt gcggcgtagc aagaaagcct cataattccg gctcttttga
      301 aacctttccc acttagcttg tctaaagcca tgtacatcaa agtcttcatt gatttcttgc
      361 cagtgtgatt ttattccaat ttgatacaac ctcgttcggg aaggagtgct gccctgcgcg
      421 aaaataacag ctttaggcat taatgcggta aaatgaacaa taatggatgc tacagtagcc
      481 aacactttat cacgatcacc attattactg accgaattat cgctggctcg actttccgcc
      541 tctagccaat cgcctaaata aagattatag tattcgtctt cgtagtccgt cagggcagta
      601 aatgttacaa ttttcttgat tctgccattt ggtcctacgc tataaaattc ataataaaaa
      661 tcatcaaccc ctttaaatat tgcgtatttc tcaagatcca tagcataagg ttattatttg
      721 cagtatgttc gaaaatggaa cacacttaaa tcggctgaat aaagtaagca tatttgcagc
      781 ggatagaaaa aagaatcagt ctgtatcccg ggttggaacg cacacctctg aaacaccagg
      841 tcatgttctt ggagaaattt cctttcgggt gctaaatttt cctaccttta tcctgctttc
      901 cggaagtatc ccgtccgcgg aaatgggcac agcccaccgg tgtggttttc gataaacaca
      961 taatcaactt aacttcttgc ctctgtgcgg acacggcatt ttaaaaagaa ggaaagttat
     1021 gtctaccgtc tcgcgggatt ttcccgctca tccgcatctc gatgttccca agaaacaagc
     1081 ccgcgaactg ctctcacagg tcagggcaag agtaaccgat gcgctcgacc gcatccggcg
     1141 tcggcatccg cattttcaat ccacagaaga cgacaccatt tccatccgcc tgaaattgag
     1201 tgatgcgcaa ttagtcattg cgcgggaata cgggttctct tcctggacac agctcaggaa
     1261 acgcattaca ggaaatacag ctgcacagct gatcgacaaa gccatccgcg ccaatgatgc
     1321 cgcggctgtt acgcaattgc tgacggctta tccaaattta ttggatgtgc cggtggtcag
     1381 cggcaactgg ggtccgccta tgagtcacgc cgccaatctg ggtcggctcg atatggtaaa
     1441 gaccatcgcg gcgctgggcg caaaagactt ccagcacgca tttgatcgtg ccttgctcca
     1501 tggcgatatc gaaattgccc gatggctgca tgagcagggc gcgcaattca cgccggggat
     1561 catcacggga tcatgtgaaa cgctcaatga acgcggtttc gcgttcctgg ttgaggccgg
     1621 ggttccgttg acggatgcaa aaggtgattc cctggccccg cttgccatga tcctggaaac
     1681 atacagtcgc aatcccgctg gtaaacatgc tatcttacga ctattccggg acagaggcta
     1741 tcaatgggcg gatacgccga tcatggcctt tcactgtggt gatctggacc ggctcaccag
     1801 tcatctgcag cgtgatccgc aggtgatcca ccggcgctgg gcctatcgcg agatctatcc
     1861 gccttcgctg gggtgtgcag atgactcccg atcgggtctg catggcactc ccatcgacgg
     1921 aaccacactg ctacacctgt cgatcgattt cgacgaacgc gacatcttcg attggctgct
     1981 ggagcatggt gcagatgtaa atgccgttgc aaccatagat aatgaaggtt ttggccgcca
     2041 cacaccgctg tataatgcac tggtgtgtca ggcctatgga gccggcagac aacgggattc
     2101 ctacatggtc cgacggctat tggagtctgg ggcggatatt catttgaggg tggatctgag
     2161 aaagtatctg gattggcgcg aagagccggg ttggcatatt gcacggaatg tcactccgct
     2221 ggagtgggcg gtcaattttc cggaaacagg ctgggtcaat cgggaagcag tcagaatgat
     2281 cgaaaatatg cgcagggaat gatctcctta ccgaatgctg cccgctgcac ccgcggcctc
     2341 attgccgcgg cagtaatgaa acgcaacccc gtgtctcatc cctgagccaa tacatagctc
     2401 gtactccttc ctccggcaga ctccttgacc aacacacctt tcgcaacgag atcattgata
     2461 tcgcggacag cagtatcgga agaggtgtcg gtcaggcgcg cccagcggga agaggtgagg
     2521 cgggtttcgg catcgtcgag aagacggcca atgatcagtc gctgacgttc gttgaattca
     2581 aaagcggcgt gaccttccca gaagctcgct tttcttaaaa tggcggcgag atggtcggat
     2641 gttaaggcct gcccaagaca atgcaggaac cattccagga acggggtaat atcggacgcg
     2701 gcggcggtgc gcgcatcggg agcattcctg aaaaatcggg catagacatc ccgctcgcgg
     2761 acaatggcct ccgacaaact ataataacga tatccgccct cttcggcgcg ggccagctgc
     2821 agatccgtga tcgcccgggc gatacgcccg ttactcccgt cgaaaggccg cacggtcatg
     2881 aaccaccagt gcgcaacacc cgcctttatt acaggatcga tgtcgccatc ggcattgaac
     2941 caatgcagga aggccctgag cttcgcgggc ccggcatgtg gtgcgggagc ctggtagtgg
     3001 acgagacccg tcgaacgccg gccaaccgcc ggcataatgg acctatgcca ctccgttagt
     3061 cgcttttcgg tgaggggctg atcatagtgc aaatgaacat cgaggatcat accggcggcc
     3121 gaaactgcgt cgatgccggc gggcgccggc acgccggaca ccaatcccag ccgggcggca
     3181 aacgtggagc gcacctggcc gggagggagc gtctcacctt cgagctccgc cgatcggacg
     3241 gcttccagcg taagcgctcc gataaaggct tcatcgcgta gagaggcggc catcgccgac
     3301 atccgacccg acagacgacc ctgccgataa cgcgactccg cggcagaccg cagcaggcga
     3361 ccggcatccc aggaaaaatc gggtttaccc ttctgtttat caacatatac ggacattcgc
     3421 cgcaaattat gcggtaaata taagcgtatt ttttcgcccc cggaaatcgc ggccatcaca
     3481 ggacccgcat ccataaaaaa tgatcgaaaa gggctaactt cccctcaaac tgcaacatga
     3541 tcacatccac ctccaatcag ctgcattggt attcgatcat cagccccaat ttcgcgaaag
     3601 cgatccggta tgccctgaac accgatttca cgggcatgga tataggaaaa tatcccatcg
     3661 acggagagaa tgtgtttgct atcgtcaatg aatatacgac aaagcccgtg tccgggtgcg
     3721 accccgagag ccatcgcgat tatgccgata tccagatcat ggtcgccggc tcggagcggt
     3781 tcggctatgc gcccctgacc gaccagcccg cttcgacccc ttataaccct gcgagagacg
     3841 tcgccttcta ttccatcgcc gaagacgaac tgaactatat cacgctcggt cccggtcagt
     3901 tcatcatctt tttcccgtcg gatatccacc agccagaggt attcgtgtcg cagccgcaac
     3961 tggtgagaaa ggtcgtattg aaagtgggtc tccgcccgat ggcggactac tcgcagacct
     4021 cggtagagtg agggcacgcc aggcaggagg catcgggccg gagtgcgcat cgggcggccc
     4081 gcagcgaata cgcgcgggtg gcgaaaatac cttattttag cacaaaccaa tctccatcga
     4141 ccatgagaat cttcttcgca ctgcttgctt tatctatctg ccccgccgct ttctcacaac
     4201 catcatccgg agtgccgacg ccgctccccg acctgatccc cgcaccggtg agcgaaacca
     4261 tcaatagcgg ccatttcatt ctaccacata tggtcttgat cgaagccggc gaccagcaag
     4321 cgttggcccg tatcctggaa gacctcagga tgcatctcag cgtccctacc ggctataacg
     4381 tgacggtgac caggcagtcg agtcgtaacg cgaccatccg gctcatcctc aatacaaaac
     4441 ccgaagcctc gctcggcagt gaaggatata ctcttaacat cacttcgaaa tccattctca
     4501 taagggccaa ccagccggcc ggtttattct atggcgttca aaccctcttc cagctgttcc
     4561 ctccggcgat cgaaagccgg ctgcaggtac accgcgattc ctgggaggcg ccctgcgtga
     4621 ccatcacgga ttacccgcgt ttcgggtggc gcggattgat gctcgacgtt tcgcggcatt
     4681 tcttcagcaa acaggaagta aaggatttta tcgatgaaat ggtgaaatat aagttcaatc
     4741 tgctgcacat gcatcttacc gacgacgagg gttggcgcgt ggagatcaag gggcttccgc
     4801 gtctgacgca ggtgggcgcc tggagtgcaa aaagagtggg caccttcggc accttccccg
     4861 acccggcacc cgacgaacca cgcgactatg gcggtttcta tacggccgac gacataaagg
     4921 agctcgtaaa atacgcgaag gaccgctttg tcaatatcct tcccgaggtg gatatccccg
     4981 gtcacagtct tgccgccatc gtctcttatc ctgagctatc gtgtacgccc ggtgcggata
     5041 aataccatgt ccgttcgggc gaaaagatca tgaactggta taagggcggt ttctcggcga
     5101 tctatgataa taccctctgc cccgccaacg agaaagtata tccttttctc gatacggtct
     5161 ttacgacgat cgcaaagctg ttcccttttc cctatatcca tgtcggcggg gatgaatgcg
     5221 ccaagaactt ctgggaacaa agcgatgccg tcaaacagct gatgcagcgt gaaaatctga
     5281 agaacatgga agaagtgcag agctatttcg aaaaacgcgt cgaaaagatc gtagagtcca
     5341 agggcaagaa ggtcatcggc tgggacgaga tcctcgaggg cggtctggcg cccaatgccg
     5401 ctgtcatgag ctggcgcgga gtcaaaggcg gcatcgccgc tgcgaaaatg ggacattccg
     5461 tggtgatgag cccgacgact tatttctatc tcgactacat gcagggcgac gcgtcgatcg
     5521 agccccccgt ttatgctaca ctccggctga aaacgaccta ttcctatgag ccggtgccag
     5581 acagtgtcga tcccaaatat atcctcggcg ggcaggccaa tatgtggaac gagcaggtct
     5641 ataatacccg gcatctgcaa tacatgatct ggccgcgcgg catggccgta gcagaagatc
     5701 tgtggtcgcc gaaagagaaa aagaactggg ataattttgc cgcaagggta gaagagggct
     5761 tccgccgtct cgatgtcgaa caggtcaaat attcgacctc gatgttcgat gcgatcatca
     5821 ccccaaagaa ggacctgacg gctttcgatt ccatcaccgt agacctggcg acggaagtca
     5881 atgggttgga tatctactat agtttcgaca attccaaccc ggataacttc tatccgaaat
     5941 ataccggtac gctaaccata ccgaaggatg cttcctggtt gaaggtcgtc acctaccgga
     6001 atggaaagca aatcgggaga cagctcaatt tgacaatcgg cgaactgcag cgccgcgccg
     6061 gtgttgcgat gcccaagcct accccggtgg cggactgatc cgaagtattc gcggaattgc
     6121 ccttgaattt tattaaattt aaggaatgaa actgtggggt ttcctgtttc cggcattact
     6181 ggctgctacc gtctcttcat ttggtcaatc caggacggat tccagttttt cgctgctttt
     6241 caacagcggg atcagtttca cgcatgccaa cgatccccat atcaaccgat ggctcgaaaa
     6301 atatggctat cctcccgagc cgcatatccc taccagcatc aatttcgaga tagcggccgt
     6361 tcccgctttt tcacgtttgc tatatacggt caagctctcc tccatcaact ccggcagcaa
     6421 cctgagctcg tataatattt ctgccggatt atacgcgtca ctgctcaaat cgaaacattt
     6481 ctttctgtat ctcggtggct cggtaggtct gcatggagat atcatcacgc tgaatggaca
     6541 actacccccg gaatacgaac agatggccgc gtcatatcat gctccgctgg cattgaggag
     6601 aagaggactt ttcgtggagc cggctgcaag ggtcttctgg tatcccattc acttccgggc
     6661 cgtacagctc ggggtctttg ccgcgctcgg atacgatgtg gacatcaatt caaaatggaa
     6721 gctgggttat tacagcaata accatggaaa atacagtcat ttccgggcgc tcggcaaacc
     6781 tggcgatcag atgaaggtca gcgagtatgg cgtcttctat attggcggtc tgagcttccg
     6841 ggtcaatctg cattaggggg gaaaaagcgg acattcccga cgagggcgcc gggccggttg
     6901 atcaaatcct cgggggtttc ccgcgctcct attcccttat tgtcgccgtt ttctcccgcg
     6961 cgggcgggct ggggatctta cgtcccgata attcggatat cagctcggcg accagcgagg
     7021 tccagccggt ttgatgactg gctccgagac cgtggctggt atccccatgg aaatattcgt
     7081 aaaacaggac cagccgttca ttccccggct gctggtagaa ccagttgtat tttccatgca
     7141 ggggtctgcc gccggatttg tcctttctga aaagactgat cacacggtcg gtgagatggt
     7201 cggcaacggc ctcaagcgtg agcttcgtgc cggaacccgc agggtattcc accatcaggc
     7261 catccccata gaacttgccg tactgacgga tgctccggat gatgaggtaa ttgatcggta
     7321 tccacacggg accgcgccag ttggagttgc cgccgaaaaa gtcggacgta gaatcacccg
     7381 ggtcatattt gatgtgataa tcgacgccgc cgatattgat gttataggga ttcccctcgt
     7441 ggtatttgga tagcgcgcgg atgccgccgg gagagaggaa ttccgcctcg ttcagcagtc
     7501 gctccaataa atagatcagc ctcttctggg gcacgagaga aagcaggatc tgctccccgt
     7561 cgctgtgctc ttcgtttggc cagtaccgtt tgttcttgat gcggtagctt tcgaaccagg
     7621 tgatgcgttt tttgaaatca ctcagcctgt gcagcatctt cttctcgatg atggatacgg
     7681 caaacagggt cgtgagtccg acgatagact ggatccgaag ctggaccggc gatccgccga
     7741 tagacagggt atcataaaag aaccggtctt cgtcgttcca catgccctgt tcgttcagcg
     7801 cctccgcgat cagcacgaaa tgctcgaaga atttggtcgc ggtatcttcg aatgtatcgt
     7861 ctttcatggc gatctcgagc gccatgtcca tcatgttgag cgcatacatg cccatccagc
     7921 tggtgccgtc ggcctgttcg agctgcatgt ctccgcccag cgcatgactg cggttgaaga
     7981 ccccgatatt atccagaccg agaaagcctc cttcgaacat gttgttcccg ttggcgtctt
     8041 tacggttgat ccaccaggtg aaattgatca gcagcttttg aaatatcttt ttaaggaagg
     8101 cgatatcgcc tttgccggta cgctcttttt cgatgcggta gacctccatg gcagcccagg
     8161 cctggacggg agggttcacg tcgctgaagt tccactcata cgccggtatc tgtccgtcgg
     8221 gtttcatata ccattcccgc atgatcagca tcagctggtg cttggcgaag accggatcga
     8281 ccagcgccat cgctatgctt tggaaggcca ggtcccaggc ggcataccag ggatattccc
     8341 atttatccgg catcgcgacg atgtcctggt tcttgagatg cttccaatcg tgattccggc
     8401 cattgagttt gcccgggttc acaggagtaa tgccgtcgct ggtggtcagc cagcgctcga
     8461 catcgaaatg ataatattgt ttactccaga gcagaccggc cagcgcctgt cgctgtaccg
     8521 cggccatgtc ggaagtgatg ttggcgggaa ggatactctg atagaaagcg tcggcttctt
     8581 ttttccgaac ctggaacacc gcattgggat cttctccgaa cggattctcc aggaccctat
     8641 tgctcagccg caggtaaagc gtcctggtct cgccaccgcc gatggtatac cgatagacgg
     8701 gcgcgaattt ggttccggat cttttatgcc gaagcgcctc tatatcgttg ccgttgatga
     8761 tcgcttcatg aaacgcgtct tttacgaagg gggtcttgtt cggcttgccg gacacctttt
     8821 cgtggttggt ctcgttttcg gtaaacagca tatcgtcagg aggctggaaa taaaaatagt
     8881 atgcccccag ccggccatgg ttggccttca ccgaatgccg gtcacggcta gcgatgacgg
     8941 gttttttctc cagaccatcg tattgccacc ggttgtagaa ccagatagtc gggaggacgg
     9001 tgatctccgc ggccttgtca tagcggttat ggatatcgat gcggatagag atgtcccggg
     9061 cagtttgttt ggcgtaggtg atcaggacat cgaaatattc gttctgatcg aagacaccgg
     9121 tgtccaggat ctcatactcc ggttcttcgc gacttcgttt ccggttttct tccagcagtt
     9181 gctgataagg gaattcctgt tgcgggtatt tgtacaggta ttccatgtaa taatgcgtgg
     9241 gaagattgtc gaggtaataa tacagctcct tcacgtcttc tccgtgatta ccttccttat
     9301 tgccaagacc aaaaagccgt tccttgagga tggggtcttt accgttccat aacgccacag
     9361 caaaacagag gttctgaaaa aaatcggaga tgccgccgag gccgtcctcc ccccagaaat
     9421 aggtcctgca atgtgcgtga tcgtagggaa aatagtgcca ggcatcgcca taagggctat
     9481 agtcctctct taccgttccc cactgtcttt cgctcaaata cggtccccac tgttctaaag
     9541 gtatcttttt agcagcgttg gctgcgagtc gttgatgttc tgcggtcata ttcttttctc
     9601 gatgccggaa gccattttcc gtggtttttc ggccgggaag ttactaaaaa agcgcccttt
     9661 ctgcccaaat tccgccgaaa acaccgaact ttatgcatac gggaggcctg aggccgtccc
     9721 aaatcatact aaacttaatc attcgttgac ttttaaagaa tttggccttg acgagaatct
     9781 acttgagggt atcgaggctt ccggatatga aaacgccacc cctgttcagg aacaggtgat
     9841 ccccctgatc ttacaaggaa aagacattat tgcgtcggcc cagacgggta cgggcaagac
     9901 cgcggcattc ctgttaccca tcgtaaacag gctgctcgcc tcgcgtcatg acgaccaggt
     9961 gaatgctctt gttatcgttc ccacccggga gctggccgta cagatcgccc agaacctcga
    10021 gggtctgtcc tattttacac ccatcagctc gattgccgta tatggcggcg gcgacggcag
    10081 ttccttctcc accgaaaagc aggcgctttc gcgcggcgcg gatgtcgtca tttgtacccc
    10141 gggcaggatg atcgcccatc tcaatatggg gtatgtgcgg acgcagggtc ttcaatatct
    10201 ggttttggac gaggcggatc gtatgctcga catgggtttc tatgacgaca tcatgaagat
    10261 catttccttc ctgccacacc aacggcaaaa cctgttgttc tcggcgacca tgccggtgaa
    10321 gatcagggac ctcgcacgga agatccttca tgacccggcc gaggtgaata tcgctatttc
    10381 caaaccccct gcgcgtatcg tgcagcaggc cttcgtggta tatgagacgc agaagatccc
    10441 gctggtaaaa tggctgttga agtcctccaa tctgaagagt acgctgatct tctgttccaa
    10501 gaagcataat gtcaaacagc tgacgatgga gctccggcgg ggtaagttca atgtgaacga
    10561 gatccattcg gacctcgagc aatccgcgcg ggaagatgtg ttgctggaat tcaggagtcg
    10621 tcgtttaccc atactcgttg caaccgacat cctttcgcgg ggtatcgata tcgacaatat
    10681 cgaaatggtg atcaactacg atgtccccaa cgacggggaa gactatgtcc atcgcatcgg
    10741 tcgcacggcg cgtgcggaga ccgacggtgt ggccgtcact tttatcagcg aaaaagagca
    10801 gaataagttt cggggtatcg aggagctttt aggccagccg gtacccaagg cggctgtgcc
    10861 cgagcagttc ggagcggctc ccgaatacaa tcccggatcg cggggcaaag gcgggggaag
    10921 aggtcgcgga cgcggaggtc ctaaaggagg tcacggtggc ggaaagggca cccatcctgc
    10981 aggccattcc gggggcggtc aaaacaagca tggtcatggc ggtcaacatg gcaaaaccgg
    11041 acatagtgga gaaggtcatg gcggacatag cgctcaccgg cggaaaccct catcatccgg
    11101 ggaacaggct cctaaacaag gataagcgcc cctccgagag ccatccagat catcacgatc
    11161 accggagagg gaagtctgga ataattcagc aaggcccagg tggcggttat gaccaccagg
    11221 ttcaaggcga tatccaatag cgaagccgca cggaaatcct tcatcatata caatgcggcg
    11281 gcgaccatga cgcccacaac tacggcattg atgccttcca gggcccggta gacggtcgca
    11341 tattttttca ggttgtgcca gatcggaaaa aagaataata ccagcaatgc gctgggcaga
    11401 aagatgcctg tcgctccgat aaagcatccc aggatctgcc aggtcgtccc tttatccttc
    11461 atggcaatac cccccatata gcttgcgact gaaaatacgg gtccgggaat ggcgcggacc
    11521 ataccccatc ccgaatagaa atcctctttg ttcatccgga tgatatgggg attgcgctgg
    11581 atgagttttt gggattgagg gcgcgccacg aattgttcgt acatcatcgg catcaggaca
    11641 ttgccgccgc cgaagacgag gctcccgaaa cgataggtgt tttcaaaaac gtttaaaggc
    11701 cggcgattgg gccagttgtt cttacgcgcc aattcgctac tcgcacccgc cagaaggaag
    11761 atgatggcaa aaagccagat atttccccat tttatctttt taggcaggac ctccacctgc
    11821 ggtatacgcc ggctgctgag gttagttacg aatccgccca aaaccagcag gatggggaag
    11881 atccatggcc attgaaacaa aagaaaggta gctatagtac tgactaccaa tataatacga
    11941 gtgatcgtgt tgcggatgga tatgcggaat gcctgttgtg cggcaaatgc gagaaagccg
    12001 atggtcatcg gaccgatgaa gcggaataag cccgtcgttc gcatcgggaa gaaggagaac
    12061 agggccatca gacaggaagc aggcagcgtc cagatgatca gggtcagcat ggcgagtacg
    12121 acgccacccc gtttgtagcc gataagggta agggtttggg tagaactggc gccgggcagc
    12181 agctggcaaa aggcgctgta gtccatcaac tcttcttcgg taatatctcc ccgccggtga
    12241 acaaaggtct tcatcatcat ccccaaatgt ccctggggcc ctccgaaagc agagatacta
    12301 tgcagcagga ccgctttgag gaaagcaata tgacggaata ggactgtcac acggccacaa
    12361 tattatcgaa ttgtcggaaa tgcgttcgaa aaatttacgg cttttgtttc ggccgcgcgc
    12421 cttattcgta tattaggaat acccaaaact tcgcaaatga aaagcccgtt ctctcttctt
    12481 gtcctcctgc tttcctgttt ttcgctggca agcgcgcaga ccaatgtcag cgccgccatc
    12541 gatgccgccg ccgataagct cgagccgcaa accatcgcct ggagaaggga ttttcatgag
    12601 catccagagc ttagcaatcg cgagttccgc acgtcgaaga tcattgcgga ctatctgcgt
    12661 tcgttgggtc tcgaagtaaa agaaggagtg gggaagaccg gcgtggtcgg cgtattgaaa
    12721 ggcgatctgc cgggtcctgt ggtcggtctg cgtgcggaca tggatgcgct tccactcgtt
    12781 gagaggacgc cggtgccgtt cgccagcaaa gtaaggacta cctatatcgg ccaggacgtg
    12841 ggcgtgatgc atgcctgtgg tcacgattcg catatggcca ttctgatgag tgtagcgaag
    12901 atcctttccg acatgcgtca tcagttaaag ggaacggtaa aatttatttt ccaacctgct
    12961 gaagaaggtg cccctccggg agaagagggc ggtgccgccc tgatgatcaa agaaggcgcg
    13021 ctcgacaatc caaaggttga cgtgctgttc ggtcttcata tcaacgcagc gacggaggcc
    13081 ggtaagataa aataccgtag tgagggcgcc atggcggctt cggattggtt tacgatacgc
    13141 gttcatggga aggggtcgca tggatcgcaa ccctggggcg gtatcgaccc gatcgtcatt
    13201 agcgcccaga tcatcgaagg actgcaaacg atcatcagcc gccaaaccga gttgacaagg
    13261 gatgccgcgg tgatatcggt atgtgtgatc aatggcggag tgcgcagcaa tatcattccc
    13321 gaagaggtgg aaatgaccgg tactattcga acgttggaca cgggtatgca gcgtgatatc
    13381 catgagcgca tgcgccgtac cgttacgcgc atcgccgaaa gtgccggggc gactgcagat
    13441 atctccatcg acaacaagac cctcatcacc tataatgatc cggtcctggt aaaacgcatg
    13501 ttgccttctc tgcaacggac cgccggcgag aaaaatatat tgtggatgga tgcgatgacg
    13561 ggttcggagg atttctcttt ttatggtacg aaggtcccgt ccttcttttt ctttcttggc
    13621 ggtatgagac cgggtcagga cccacatacc accgcgccgc atcatacgcc tgatttttat
    13681 atcgatgaga gtgggttcaa attgggggtg aaagcgttct gccatctggt tttcgactat
    13741 gcggatagcg tgaccttgaa gcttgcctga attgtattat atataaatga cttatttatg
    13801 gatgttgagt cgatacggga atactgtctg gcgaaggatg ctgtggagga aggtttccct
    13861 tttggcgaga ccacgttggt gtttaaggtc cagggggcga tctttctatt gatagggctg
    13921 gataatcccg agttgcagtt caacgtcaaa tgcgatcctg agcaggcgat cgaatggcgc
    13981 gaacagtatg cggcggtgca gcccggatat catatgaata agcggcattg gaatacggtc
    14041 atcgtcgacg ggactatccc agcccgtatt ctgcgtcaga tgatcgatga ttcatataga
    14101 ctggtcgtgc aaaagctgcc aaagaagctg cggccgccgg gcgcctctca cctgtagtat
    14161 ccattccttt caatctcttc ccgaacagta tcggggacca ggtaccggat cgattttgat
    14221 tcgcggatat ttttgcggat gtctgtcgag gagatgggca gtaatggggc gtcgagatca
    14281 tgtatctctt ttgcgtcttc atatagagga agggagaggt ggcccgggcg tttgtagaca
    14341 taaaccggaa aattccgaag cagccattcg ccgtttttcc atttgggcag attttgaaag
    14401 ctatcgctgc ccatgatgat cgaaaattca ttcacaggat atttctcctg caaataagcc
    14461 aaagtgttga cagtgaaaga cggtttcggc aaacggaact ctatatcgga agcccgcaga
    14521 tccctggctc cctcgatagc cacctgtaca agatggagcc tgtgatattc attcaaaaga
    14581 gaggcggtag gcttgagcgg gttctgcgga gatactacaa accacacctg ttgaatgttg
    14641 gtatgctgta aaacataggt agctatcaat aaatgaccat gatggatggg gttgaacgat
    14701 ccgaagtaaa gtccaacctt catctgtctc cctcctccat gaaaatattc tccgcctcgt
    14761 caagacgcaa acttaaatta tagcccgcca ccgtgatcga aatagggtca cccagcgggg
    14821 cgatttgttc taccctgacc aattctcccg gcacacagcc catctccatc aatttcaaaa
    14881 aaagatcatt tttttcaaac gaatggatcc tggcgatcgt ccccggctct aattctgata
    14941 atctccgcat ttttatgaat ttcaggctta tcttaggtaa aattatgact attccgacca
    15001 ttcaattcaa ctttctcgaa tccatcgcac tgagggacag gaggaagttg aagcttttcc
    15061 tggttcggct tttcaaaaaa gaagggcgac ccctggcgga attgcagtat atcttttgtt
    15121 cggacaaata tctcctggct atcaaccggc aatacttgaa tcacgacgat tatactgata
    15181 ttatcacctt cgatctttcc gaaaagaacc atccaataaa cgccgaaata tatatcagtg
    15241 ttgaccggct gagggaaaac gcgctgaagt atgacagctc attaaagaaa gagttactcc
    15301 gggtcatgtt tcatggcgcg cttcatttgt gcgggtataa ggacaagacc gccacggacc
    15361 agggggaaat gcgaaagatg gaggaaaagt accttcggtc gtatgatcgt tccacgtgaa
    15421 acatgaagaa tcttaaataa aaggcataac tagtcgttcc ccgtggaacg ttccatccgg
    15481 caaattattt atcggtaatt ttgcatcatg tttcaggaat acgatgtgat tgtggttgga
    15541 gctggacatg ctggttgtga ggcagcggca gcagcggcca atctggggtc gagcgttctg
    15601 cttattacca tgaatatgca gaccattgcc caaatgagct gcaacccggc catggggggc
    15661 atcgccaagg ggcagatcgt ccgggagatc gatgctttag gcggatattc cggtatcgtc
    15721 accgacaaaa gcatgatcca gtttcgtatg ctgaatcgta gcaagggtcc cgcaatgtgg
    15781 agtccccggg cgcaaaacga ccggatgcta tttgccgcaa cctggaggga aatgctggaa
    15841 aagacaccaa acgtagattt ttatcaagac atggtttcct ccctgattat caaggaaggc
    15901 agggtttgcg gggtagtcac gggtcttggg cacaccattt cagccaaagc catagtcgtc
    15961 acaagtggca ctttcttaaa cggcatcatc catataggcg agaaacgttt tggagggggt
    16021 agggtagcag aaaaagccgc taccgggatc accgaacaat tggtatcaga agggttcgaa
    16081 agcaatcgtc ttaagacagg cacacctcct cgtatcgacg gaagaagtct cgattacagc
    16141 aagatggaag aacaacgcgg tgatgaaata atcaccggct tttcctacct ggatcttcca
    16201 aaaccctccg aacaacgca
//
LOCUS       DASYSV010000528         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2287888, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000528 DASYSV010000000
VERSION     DASYSV010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287888"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VGQ51_16540"
     CDS             complement(<1..385)
                     /locus_tag="VGQ51_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV9038246.1"
                     /translation="MSNKYASEINKRRTFAIISHPDAGKTTLTEKFLLFGGAIQTAGA
                     VKSNKIKKHATSDFMEIERQRGISVATSVMSFEYKGVLINLLDTPGHKDFAEDTYRTL
                     TAVDSVILVIDSVNGVEAQTRRLMEV"
     gene            complement(433..1947)
                     /locus_tag="VGQ51_16545"
     CDS             complement(433..1947)
                     /locus_tag="VGQ51_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEV9038247.1"
                     /translation="MSTIRRQSIISSVIVYSGFALGAVNTLLYGRGLAPDEYGLITGI
                     FISVGNIMFAVANPGAVAYISKFYPYYHDHLPARENDLISRALFFSLVCFALVTLGGI
                     VFKPLIIRKFGHNSAELVHYYYWIFPFALGLTLFSVLEAYAWQVKASVVTSYLREFQW
                     RVFNLVLIVLLFLGVLTNFSVFVKSYAFTYLLLALILFFYLYSKGHLHLTFSVSRVTK
                     KFRRKIRALMLLTWSGAVLFNLSFFFAAIVIAAVVPAGLTAVGIFALAQNIASFIQAP
                     QRAIAAASLGPLSRAWKDKDIGRIQRIYSRSSINQVIFSVGMFVLIALNFRDGILTLG
                     FKQDYLAGLPIFLIIGLTRIVDMGTGVNTQIIVTSVYWRFEFISGMILMTLTIPLNYL
                     LARQMGVVGPAIADLVTFSVYNAIRGIFLYRRFNLQPFNRNTIYTLLLGAGTFASGYW
                     LFHRHQGFIWLVLRSTAVIVLYVAGVVLLRLSDDIGPVWNTVKKRLGLAGTRTP"
     gene            complement(1961..2446)
                     /locus_tag="VGQ51_16550"
     CDS             complement(1961..2446)
                     /locus_tag="VGQ51_16550"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEV9038248.1"
                     /translation="MQRFNRGVGRVTCCGLLVMIGVASCKQPEAPEYYGFQNLQIGAA
                     TGSQTTVSTIVKLYNPNPYNLELRRAEVDVSINGRHAGHSLLDSTIRIPRKDTFYVPI
                     SMQVDLQAIMSNALQTLLSREATVSLDGRVKIRKGMLTFNRSFHYDGKQDLNALLQQG
                     F"
     gene            2511..>2999
                     /locus_tag="VGQ51_16555"
     CDS             2511..>2999
                     /locus_tag="VGQ51_16555"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEV9038249.1"
                     /translation="MASIPERIKEFNESRLQEFTAMKYELMAGNAFRFFRGTCHLFYE
                     DLRRNDSLPLSPATWISGDLHLENFGTYKGDNRLVYFDLNDFDEGVLAPAAWEVVRMV
                     TSIFTGCESLGISEKETMELARLFLEVYAATLDKGKARYLEIETAHGIVRLFMEKICQ
                     RKQ"
BASE COUNT          786 a          796 c          802 g          615 t
ORIGIN      
        1 aaacttccat caatcttcgc gtctgcgcct ctacgccgtt gacgctgtcg atcaccagga
       61 tgacgctgtc cactgccgtc agggtccgat aagtgtcctc ggcgaaatcc ttgtgcccgg
      121 gggtatccag gaggttgatc agcacgcctt tgtattcaaa gctcattacg gacgtcgcta
      181 ccgaaatacc gcgttgacgc tcgatctcca tgaaatcgct ggtcgcgtgt tttttgatct
      241 tattgctttt gacggcgccc gccgtctgga tagcgccacc gaaaaggaga aatttctcgg
      301 tcagggtcgt tttacctgcg tcggggtgag atatgatggc gaaagtcctg cgtttgttga
      361 tctcgctggc gtacttgttt gacataaatg ccggcaaagt tacgaaaaat tagcgggtcc
      421 agcctccgga atctagggcg tgcgggtccc ggccagacca agtcttttct tcacggtatt
      481 ccataccgga ccgatatcat ccgacaaccg cagcaagacg acacccgcaa cataaaggac
      541 gataacagcc gtcgagcgga ggaccagcca gataaaaccc tgatgacgat gaaaaagcca
      601 gtaaccggag gcgaaggttc cggccccgag caacagggta tagatggtgt tcctgttgaa
      661 aggctgcagg ttgaatctgc ggtaaaggaa tatcccgcga atagcgttat aaacggagaa
      721 cgtgaccaga tcggcgatcg caggtcccac cacccccatt tgccgggcca gcagatagtt
      781 aagcgggatc gtaagggtca tcaggatcat cccgctgatg aattcgaaac gccagtaaac
      841 ggatgtcaca atgatctggg tgttcacgcc cgtgcccata tcgacgatcc gcgtcagccc
      901 gatgatcaga aaaatgggca gaccggccag atagtcctgc ttaaaaccca aagtaaggat
      961 gccatccctg aaattcaggg caatcagcac gaacatgcct accgaaaaga tcacctggtt
     1021 gatcgagctc cggctataga tgcgctgtat cctgcctata tccttatcct tccaggcccg
     1081 cgacaatggg cccagcgacg cggcggcgat agcacgttgc ggcgcctgga taaagcttgc
     1141 gatattctgc gctaacgcaa aaatacctac tgcggtaagt cccgccggga cgacggccgc
     1201 gatgactatc gccgcaaaga aaaaggacag gttgaacagg acggcgccgg accaggtcag
     1261 cagcatcaat gccctgatct tccgtctgaa ctttttcgtc acccggctga ccgaaaaggt
     1321 gagatgcaga tgccctttcg agtagagata gaaaaaaagg atcagggcaa ggagcagata
     1381 cgtgaaagca tatgacttga caaaaacgct gaaattggtc agtacaccca aaaataaaag
     1441 aacgatcagc accaggttga acacccgcca ctggaattcg cgaagataac tggtaaccac
     1501 agacgccttg acctgccagg catatgcctc gagaaccgaa aagagggtca ggcccagcgc
     1561 aaaaggaaag atccagtaat aataatggac cagttcggcg gaattatgcc cgaatttccg
     1621 aatgatcagc ggtttaaaaa cgataccgcc aagggtcacc agggcaaagc acacgaggga
     1681 aaaaaacagc gcccggctga tcagatcatt ttcccgggcg ggaagatgat catgataata
     1741 aggatagaat ttcgaaatat aggcgaccgc accgggattg gctacagcga acataatgtt
     1801 gccgacggaa ataaagatcc ccgtgatcag accatattcg tccggagcaa gccctcttcc
     1861 gtagagcagg gtattcaccg cccccagggc aaaaccggaa tacacgatga ccgaagagat
     1921 gatgctttgc cggcggatgg tgctcatatc gggaattgaa tcaaaatcct tgttgtaaca
     1981 gcgcgttcag atcctgctta ccgtcataat gaaacgagcg attgaaggtc agcatccctt
     2041 tcctgatctt tacacggcca tccaggctga cggtcgcctc tctgctaagc agggtctgca
     2101 gcgcattgct catgatcgcc tggagatcca cctgcatcga aatgggcaca tagaacgtat
     2161 cctttcgcgg gatccggatc gtcgaatcca gcagtgaatg accggcatgc cgcccgttga
     2221 tggagacatc cacctccgcc cggcggagct cgaggttata gggattggga ttatagagct
     2281 tgacaatagt ggatactgtg gtttgcgatc ccgtcgccgc cccgatctgc aggttctgga
     2341 acccatagta ctccggcgcc tccggctgtt tgcacgacgc gaccccgatc atcacgagca
     2401 ggccgcagca ggtcacccgc cccacgcccc tattgaatct ttgcatgagg tgcaagataa
     2461 ggggcattta ggaattaggg aatattaagt atctttctaa aaagaatcgc gtggcctcca
     2521 tccccgagcg catcaaggaa ttcaacgaga gccggttaca ggaatttacg gcaatgaaat
     2581 atgagctcat ggccgggaac gcctttcgtt tcttcagggg cacctgtcac ctgttctatg
     2641 aagacctccg ccggaatgat tctctccccc tttcgccggc cacctggatc agcggggacc
     2701 tacatctgga gaatttcggg acctataagg gtgacaacag gctggtatat ttcgatctca
     2761 acgatttcga cgaaggagtg ctcgccccgg ccgcctggga ggtggtgaga atggttacga
     2821 gcatcttcac cggttgcgag tcgcttggca tcagcgaaaa agaaaccatg gagctggccc
     2881 ggttgttcct cgaagtgtat gccgcaacgc tcgacaaagg aaaggcccgc tacctggaga
     2941 tcgaaacggc gcatggcatc gtcaggctct tcatggaaaa gatctgtcag cggaaacaa
//
LOCUS       DASYSV010000529         3525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1134529, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000529 DASYSV010000000
VERSION     DASYSV010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134529"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VGQ51_16560"
     CDS             complement(<1..751)
                     /locus_tag="VGQ51_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038250.1"
                     /translation="MDTIHGTNYEVLRRKRKMLLWLPVILIPLLAVGFYALGGGREIH
                     VGVAFTKGLNMSLPDAKFNTKKKTLTKMGFYRESEQDSIRLRERRKMDPYYGWKDSGM
                     GPASGGNEVVRGGLPGTPGAGGLKLDPTATDTQAGQLKRTLELLKGALDRQQQELMAD
                     GQPPRRMTYQGTRERPSMPLAPAPFRSSQDDPELDKLNSLMDKVLKVRYPAEAAPRDT
                     MPELPVLRLSIPIQEEIMTTLPADDKEPIETG"
     gene            complement(797..1213)
                     /locus_tag="VGQ51_16565"
     CDS             complement(797..1213)
                     /locus_tag="VGQ51_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038251.1"
                     /translation="MRTRMNGARFPVELIGKAIANSRPVRSVVQKLKHWLVQADISQG
                     QAIRMMMAIGGLIVILVLIGLGRHMAPHYSAIQPVVTLSRAPQQPRAKAFTVLWDSLM
                     ADPATKRRWDSLITLRPGLMDTVRRLERMDSAVRQR"
     gene            complement(1225..1842)
                     /gene="traK"
                     /locus_tag="VGQ51_16570"
     CDS             complement(1225..1842)
                     /gene="traK"
                     /locus_tag="VGQ51_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraK"
                     /protein_id="HEV9038252.1"
                     /translation="MFPKTRNLESSFRLIRGTCMLVIVGAFFLCGYMFFTASQLVSKA
                     QNRIYILSGAKAMEAVAGERKDNIPVEARDHIRTFHLLFFTLDPDEKVINDNLSRALY
                     LADGTAKRVYENLKESAYYAGVISANISQRLTVDSLRLDLSAYPYYFRVYGQETIIRT
                     TSIVTRDLITEGYLRDVSRSDNNPHGFLIERWNIVENRDLKVEQR"
     gene            complement(1848..2849)
                     /gene="traJ"
                     /locus_tag="VGQ51_16575"
     CDS             complement(1848..2849)
                     /gene="traJ"
                     /locus_tag="VGQ51_16575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transposon protein TraJ"
                     /protein_id="HEV9038253.1"
                     /translation="MADGPITSISAGIGGLQTTLENVYNTMMGNCGELMGIGQGLAGF
                     GALLYIGFRVWGHLARAEAIDLFPLLRPFGLGLIIALYPGFIGAVNGLLQPTVSGTAA
                     LVNDSNAAITLLLQQKEAAIEQSTDWQMFVGPDGSGSESKWEQYSGEAETGVFSGLTN
                     AVKFQLARISYNFRNGVKVVLSEVLQIVYEAAALCINTLRTFELLLMAILGPLVIGLS
                     VYDPFRSVLSAWLGRYINVFLWLPVANIFGSLCGQIQAEMIKIDIAQIQASGSTAFSS
                     TDIAYLIFLIIAIIGYFCVPSITNHIIHVFPSGGGAMLSKVSGGPAAVAAGPEQTTK"
     gene            complement(2861..3508)
                     /locus_tag="VGQ51_16580"
     CDS             complement(2861..3508)
                     /locus_tag="VGQ51_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEV9038254.1"
                     /translation="MRRIRFLRNWVWVIILLLPAWVRAQSVADDIQQLLLDVQKLAQM
                     KQILSAMYSEYTMLQQGYEQIKGLSQGTFSLHKTFLDGLLLVSPAVSSYPKVTDIISK
                     EASLIGSYNAASGYFRGSGAFTSQELDGFSANYGSLLQRGQKDLDELTMVMTDGELRM
                     SDAERLAAIDRIDADMTRQLTLLHAFNNMAALQAAQRARAAGEVGTVRGLYGITP"
BASE COUNT          778 a         1157 c          787 g          803 t
ORIGIN      
        1 acccggtctc gataggctcc ttgtcgtcgg ccggcaacgt cgtcataatt tcttcctgta
       61 ttgggatgga gagccgcaac accggcaatt ctggcatagt atcgcgaggc gccgcttccg
      121 ccgggtaccg caccttcagc accttatcca tcaggctatt caacttgtcc agttccgggt
      181 catcctggct gctccggaaa ggcgccggag caagcggcat tgatggtcgt tcccgtgtcc
      241 cttgatatgt catccgccgg ggaggctggc catcagccat caactcttgt tgctgccggt
      301 cgagcgctcc tttcaaaagt tcgagcgttc gctttagctg ccccgcttgt gtatccgtag
      361 ccgtcggatc gagcttcaac ccgccagcgc caggggttcc gggcaacccg ccccgaacaa
      421 cctcgttgcc accggacgcg ggccccattc ccgagtcctt ccatccataa tagggatcca
      481 tcttccgcct ctctctgagc cggatggaat cctgctcaga ctcccggtaa aagcccatct
      541 tcgtcagtgt tttcttttta gtattgaact tcgcatccgg caggctcatg ttcaggccct
      601 tggtaaaggc aaccccgaca tgtatctccc ttccgccacc cagcgcataa aagccaaccg
      661 cgagcagcgg gatcaggatc accggcaacc acaagagcat ctttcttttc cgtcgcaaaa
      721 cttcgtaatt cgtaccgtgt atcgtatcca tagcgcataa tttttaacat tcaacaaacc
      781 attgccgggg acagcctcac cgctgccgca ccgccgaatc cattctttcc agccgtctta
      841 ccgtatccat cagacctggc ctcagcgtta tcaggctgtc ccatcgcctc ttggttgccg
      901 ggtcagccat caggctatcc cacagcacag taaatgcctt tgcccgcggt tgctgcggcg
      961 ctctgctcaa cgtcaccacc ggctggatcg ccgaataatg cggcgccata tgccgtccaa
     1021 gaccgatcag caccaaaatg acaatgagtc cgccaattgc catcatcatt cggatagcct
     1081 gcccctggct gatgtccgcc tgcaccagcc agtgttttaa cttctgcact accgatctca
     1141 ctggccggct attagcgatc gctttgccta tcagctccac cggaaaccgc gcgccgttca
     1201 ttctcgttct catgatttat ttttttatcg ttgttccact ttcaggtccc ggttctccac
     1261 gatattccac cgctcgatca gaaacccatg cggattattg tccgaccggg acacgtcgcg
     1321 caaataccct tccgttatca ggtcgcgggt cacgatgctc gtcgtccgga tgatcgtctc
     1381 ctgcccataa acccggaaat aataaggata ggccgaaagg tccagtcgca gactatccac
     1441 ggtcaatcgc tgactgatat tcgcgctgat cacccccgca taatatgccg actccttcag
     1501 gttctcatag acccgcttgg cagtcccatc cgccagatac agcgccctgc tcagattgtc
     1561 attgatcacc ttctcatccg gatcgagcgt aaagaacaac aaatgaaagg tccggatatg
     1621 atcccgcgcc tccacgggaa tattgtcctt acgttctccc gccaccgctt ccatcgcctt
     1681 agcccccgac agaatataga tcctgttttg cgccttactc accagctggc ttgccgtaaa
     1741 gaacatatac ccgcaaagaa aaaatgcgcc cacgatcacc agcatacaag tcccccggat
     1801 cagccggaat gaagactcca gatttctcgt ctttggaaac atacccttca tttagtcgtt
     1861 tgctcagggc ctgcagccac cgccgcagga ccgcccgaca ccttactcag catcgcaccg
     1921 cctcctgagg gaaaaacatg gataatatga ttggtgatcg agggtacgca gaaatacccg
     1981 atgatcgcaa tgatgaggaa aatgagataa gcaatgtctg tcgagctgaa cgccgtactg
     2041 cccgacgcct gtatctgggc gatgtcgatc ttgatcatct cggcttgtat ctgtccgcac
     2101 agcgagccga agatattggc gaccggcaac caaaggaaca cattgatgta ccggcccaac
     2161 caagcggaca gcacggaacg aaacggatca tacacggaca acccgataac cagcgggccg
     2221 aggatcgcca ttagtagcaa ctcgaacgtc cgcagcgtat tgatgcacag tgccgccgct
     2281 tcataaacga tctgaaggac ctcggacagc accaccttca ccccattcct gaaattatag
     2341 ctgatccgcg cgagttggaa cttcaccgca ttcgtcagtc cggaaaacac tcccgtctcg
     2401 gcctccccgc tatattgttc ccatttcgac tcgctgccac tcccgtcggg cccgacgaac
     2461 atctgccagt ccgtcgattg ttcgatagcc gcctcctttt gctggagcaa aagcgttatc
     2521 gccgcgttcg aatcgttcac cagcgccgcg gtcccgctga ccgtcggctg tagcaacccg
     2581 ttgactgccc cgataaaccc gggatacagc gcgatgatca gcccgaggcc aaacggtctc
     2641 agcaatggaa acagatcgat tgcctccgcc ctcgccaggt ggccccatac ccggaagccg
     2701 atatacagta gtgccccgaa ccccgccagc ccctgcccga tacccatcaa ctctccgcaa
     2761 ttccccatca tggtgttgta gacattttcc aatgtcgttt gcaacccgcc gataccggcg
     2821 gatatgctgg tgattggtcc gtccgccata ctaactcttt ttaaggtgta atcccataaa
     2881 gtcctcttac cgtacccact tccccggccg ccctggcccg ctgtgccgcc tgcaacgccg
     2941 ccatattatt aaaggcatga agcaaggtca actgtcgggt catatccgca tcgatccggt
     3001 caattgccgc caatcgttcc gcatcgctca tccgcagctc gccatccgtc atcaccatgg
     3061 tcagctcgtc gaggtccttt tgcccccgct gcaaaaggct cccataattc gccgaaaagc
     3121 catccaactc ctgagaggta aatgccccgc tgccccgaaa atacccgctc gccgcattat
     3181 agctcccgat caggctcgct tccttgctga tgatatccgt cacctttgga taggaactca
     3241 cggcggggct taccagtagc aagccgtcca aaaaagtctt gtgtaacgaa aacgtcccct
     3301 gactgagccc cttgatctgt tcgtatccct gttgcagcat cgtatactca ctgtacatcg
     3361 ccgataagat ctgtttcatc tgtgccagtt tctgcacatc gagcaacaat tgctgaatat
     3421 cgtccgccac cgactgcgcc cgtacccagg ccggcagcag caaaatgatc acccataccc
     3481 aatttctcaa aaatcttatc cttctcacga ttcacatttt ttcca
//
LOCUS       DASYSV010000530         4268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1771646, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000530 DASYSV010000000
VERSION     DASYSV010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771646"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VGQ51_16585"
     CDS             complement(<1..1333)
                     /locus_tag="VGQ51_16585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV9038255.1"
                     /translation="MILRVKESFLLFLLLLLLVSGIRAQNVFTIDNVPVSRDEFLRAY
                     NKNNMAQKPSEKSYRDYLDLYVRYKLKVRAAFDARLDTLPTQRAELQNFRSQVAESYL
                     KDDTSLSRLVKEAFERGQTDIRLSHILIPLPKNPTPADTLKAYEKAKMAYEALKKGKK
                     FSEVAIQYSEDPSVRTNGGQIGYITVFTLPYELETLAYSTPPGHFSKPYRTRGGYHIF
                     RNDGERKALGRIRVAQILIAYPPSATNLIRQSIRRRADSIYSLLAGGADFATLARTYS
                     GDNLSYQAGGELPEFGIGHYDSAFEATAFALNRDGAYSRPLESVYGYHIIRRIARRPF
                     PGRMDSVIAAQLRQQVMNDPRIEISRKTLLNKILQQVNYRRGDVSDADLWAFTDSAML
                     NVGLSGFRGLTRTTALFSFSRQSYTIREWLDYFRSVRGQRPGGAQADKDLYD"
     gene            complement(1335..2774)
                     /locus_tag="VGQ51_16590"
     CDS             complement(1335..2774)
                     /locus_tag="VGQ51_16590"
                     /inference="COORDINATES: protein motif:HMM:NF028437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HEV9038256.1"
                     /translation="MRKILSSLLIATCFLQAYSQDGYSIHLHLKPYTAGKVYLGYYYG
                     KIKALADSAMLSATGDGIFSGKEKLPGGVYFIVSPNRSILFELLIDSVQHFSVAADTS
                     SLPASIVFTGCPDNTVFQTYTRYTAAKGQEIIAAQKELATKRSKADSTVIREKLKSLN
                     AGLQQYRDNLAAKYPSSLLAILFRLLKEPEVPPASKQPGGRYDSGFAYRYFKSHYWDG
                     VDFTDPRLTRTPIFEPKLDKYFHDLVPPEADSIEREADMMLLQARVSPSMFQYLMVYF
                     VQKYVNPEYMGQDAVFVHLFEKYINTGETEFFTEKYRKFLNDRAYSLMANLIGQPAAN
                     LEMVDTAGTPRPLYGVEAPFIVICFWDPTCSHCKDIVPRLDSIYKAKWKAEGVRIYGV
                     MVDGGREQWLQFIKDHNLTDWIHVYETAAHADSTEKAGQPSFRQLYDVYQTPILYLLD
                     SNKRIIAKKLSYLQLDQVIDLKLRQKKSN"
     gene            2806..4221
                     /gene="rlmD"
                     /locus_tag="VGQ51_16595"
     CDS             2806..4221
                     /gene="rlmD"
                     /locus_tag="VGQ51_16595"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV9038257.1"
                     /translation="MRKRNKDRNIVLSDIVVEDYAAEGKSIARVDGKVIFIENAIPGD
                     VVDIRLSKNKKDWAEGHITAFQRYSADRVEPFCPHFGVCGGCRWQMLPYSLQLKYKQQ
                     QVEDVLRRIGKVTLPGINPILGAAETTRYRNKIEYTFSNRRFLLPRELNDPSVSPVRN
                     VAGFHARGLFDKVVDIDTCYLQEEPTNRIRKAVRDFGLREQLPFYDIREHNGWLRNLQ
                     LRICTTGEIMANLVLGYEDEEHTRRLSDLLLQQFPELTTLLVTINTKWNDSITGLDPR
                     VIYGKGYVMEALEDFRFKIGPLSFFQTNTRQGERLYQITREYAELAGHETVYDLYCGT
                     GSIGIFVSRQASRVVGVEVVEAAIDDARENAELNGIAHARFFAGDVVDICRDEFFEQQ
                     GRPDVIITDPPRAGMHEGLVNKILDMAAPTVVYVSCNPATQARDLHLLDTKYAVTRVQ
                     PVDMFPHTHHIENIVQLKLRG"
BASE COUNT         1017 a         1112 c         1154 g          985 t
ORIGIN      
        1 ggtcatacag atccttgtcg gcctgtgcac cgcccgggcg ttgaccgcgt accgatcgga
       61 aataatccag ccattccctg atcgtataag actgcctgct aaaagagaaa agcgcggtcg
      121 tccgtgtcag tccccggaag ccggatagcc cgacatttag catcgcgctg tccgtaaatg
      181 cccaaaggtc ggcatcggag acatccccgc gacggtaatt cacctgctgt aatattttgt
      241 ttaacaatgt cttacgagaa atttctatcc gcggatcgtt catcacctgc tgtcttagct
      301 gggcggcgat cacagaatcc atccggccgg ggaatggcct gcgtgcaatc ctcctgatga
      361 tatgatagcc ataaacgctt tccagcggcc ggctataggc cccatccctg ttcaatgcaa
      421 aagctgtggc ttcgaaagca gagtcataat gcccgatacc gaattcggga agctcgccgc
      481 cggcctggta agacaggttg tcgccgctat aagtcctggc cagagtggcg aaatcagcac
      541 cacctgccag cagggaataa atgctatcgg ccctccggcg gatggattgc cggatgaggt
      601 ttgtcgccga cggggggtat gcgatcagga tctgagcgac cctgatcctt cccagcgcct
      661 tacgctcgcc gtcattccgg aaaatatgat acccgcccct ggtacgatac ggtttggaga
      721 aatgaccggg cggcgtggaa taggccaggg tttccagctc ataagggagc gtaaatacag
      781 tgatataccc gatctgaccc ccattggttc tcaccgaagg gtcttcggaa tattgaatgg
      841 ccacctcact gaacttcttt ccctttttca gcgcctcata agccatcttc gccttttcat
      901 aggctttgag cgtatcggca ggcgtcgggt tcttcggcaa agggatcagg atatgcgaaa
      961 ggcggatatc ggtctggccg cgttcgaaag cttccttcac cagccgggag agactcgtat
     1021 catccttcag ataagactcc gctacctgcg aacggaaatt ctgcagttcg gcgcgctggg
     1081 tgggaagcgt gtccagccgg gcatcgaaag cggcccttac cttcagctta taccggacat
     1141 acagatcgag gtaatcccgg tatgacttct cgctgggctt ttgagccata ttgttcttat
     1201 tataggcgcg caggaactcg tccctcgata cggggacatt gtcgatggtg aacacatttt
     1261 gcgcacggat gccggagacc agcaggagaa gaaggagaaa aagtaagaac gactctttga
     1321 cccgtaggat catatcaatt ggattttttt tgtcgcagct tcagatcgat cacctgatcc
     1381 agctgcagat aagaaagttt tttagcgatg attcgcttgt tactatccag caaatagaga
     1441 atgggggttt gatacacgtc gtacagctgc cggaaggaag gttgacccgc tttctcggta
     1501 gagtcggcgt gcgctgcagt ctcataaacg tggatccaat cggttaaatt gtgatcctta
     1561 atgaattgca gccactgctc tctgccgcca tccaccatga ccccatagat cctgacccct
     1621 tcggccttcc acttggcctt gtagatggaa tcgagccggg gaacgatatc cttgcaatga
     1681 ctacaggtag ggtcccagaa acaaattacg atgaagggag cctcgacgcc atagagcggt
     1741 ctgggcgttc ccgcggtatc gaccatttca agattggccg cgggttgccc tatcagattg
     1801 gccatcaggc tataagcgcg gtcattcagg aatttccgat acttctccgt aaagaattcc
     1861 gtctcacctg tattgatgta tttctcgaat agatgaacga acacggcgtc ctggcccatg
     1921 tactccgggt tgacatattt ctgaacgaaa tagaccatca ggtactggaa catagaggga
     1981 ctgaccctgg cctgcaacag catcatatcg gcttcccgtt cgatcgaatc cgcttccggc
     2041 ggaacgagat catgaaaata tttatccagc tttggctcga aaataggcgt ccgtgtcagc
     2101 cggggatccg taaaatctac gccatcccaa tagtggctct tgaaataccg gtaggcaaat
     2161 ccgctgtcat accgtccacc gggctgtttc gacgctggcg gcacctcggg ctccttcaac
     2221 agccggaaaa ggatcgccag cagggaggaa gggtatttgg cggcgagatt atcccggtac
     2281 tgttgcagcc cggcattcag ggatttaagc ttctccctga tgaccgtcga gtccgctttc
     2341 gaccgtttgg tggccagctc cttctgagcg gcgatgatct cctgaccttt ggcggccgtg
     2401 taccgggtat aggtctgaaa gaccgtatta tcgggacatc cggtgaagac gatactggca
     2461 ggcagtgagg aggtatccgc ggcgacactg aaatgctgga cactgtctat cagcaactcg
     2521 aaaaggatcg accggttggg actgacgata aaatagacgc cgccgggaag cttttctttc
     2581 ccgctgaaga tgccatcccc ggtggcgctg agcatcgccg aatccgccag cgcctttatc
     2641 ttcccatagt agtatcccag gtaaaccttg ccggcagtgt aaggcttcag atgaagatgg
     2701 atggaatagc cgtcctgtga ataggcttgc aaaaaacagg tggcgattag gagggacgac
     2761 aggatctttc gcatagacgg gtaaaattaa ccataaattt gcaccgtgag aaaaagaaac
     2821 aaggacagga acatagtgtt atccgatata gtcgtcgaag attatgcagc cgagggaaaa
     2881 tctatcgcca gggtcgacgg caaggtcatc ttcatcgaga atgccattcc gggggatgtc
     2941 gtggacatcc gtctttccaa aaacaaaaaa gactgggccg aaggccatat taccgccttt
     3001 cagcgctatt ctgccgaccg tgtagagcca ttttgccctc atttcggggt ttgcggcggg
     3061 tgtcgctggc agatgctgcc ttattccctt cagttgaagt acaaacagca gcaggtggaa
     3121 gacgtcctgc gacggatagg caaagtaaca ctgcccggga tcaatcctat attgggcgct
     3181 gccgagacca cccgctatcg taacaagatc gaatatacct tctccaacag gcgttttctg
     3241 ctaccacgcg agctgaacga tccctcggtg tcacccgtcc ggaacgtagc cggtttccat
     3301 gcccggggcc tgttcgataa agtcgtcgac atcgatacct gctacctgca ggaagaaccg
     3361 accaaccgca tacgcaaggc cgtcagagac ttcggtctcc gcgagcagct gcctttctat
     3421 gatatcaggg aacacaacgg ctggctccgc aatctccagc tgcgcatctg taccaccggg
     3481 gagatcatgg ccaatctggt gctgggctat gaagacgaag aacatacccg ccggctttcg
     3541 gacctcctcc tgcaacaatt tcccgaactg accactcttt tggtgacgat caataccaaa
     3601 tggaacgaca gtatcaccgg tctcgatccg cgggtcatat acggaaaggg atatgttatg
     3661 gaagcactgg aggactttcg gttcaagatc ggtccgctgt ctttttttca gaccaatacc
     3721 cggcagggtg agcgacttta ccagattaca agggagtatg cggaattggc ggggcatgag
     3781 acggtctatg acctgtattg tggtacgggt agcatcggta tcttcgtaag ccggcaggcc
     3841 tcacgggttg tcggcgtgga ggtggttgag gccgcgatcg atgatgcccg ggagaatgcg
     3901 gaactcaacg ggatcgccca tgcacggttt tttgcaggcg acgtcgtgga tatctgccgg
     3961 gacgagttct tcgaacagca gggccgtccg gatgttatca ttaccgatcc gccccgggcc
     4021 gggatgcatg aaggcctggt gaacaagata ctcgatatgg ctgcccccac cgtggtttat
     4081 gtgagttgta atcccgctac acaggcgcgc gatctgcatt tgctggacac gaaatatgcc
     4141 gtgactcggg tccagccggt ggatatgttc cctcatactc atcatattga aaatatcgtg
     4201 caattgaaat tgaggggtta atcgtaaatt gtgagttaaa taaaaattag aaaaagacca
     4261 gtatgcca
//
LOCUS       DASYSV010000531         6158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_234022, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000531 DASYSV010000000
VERSION     DASYSV010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234022"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VGQ51_16600"
     CDS             complement(<1..1364)
                     /locus_tag="VGQ51_16600"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEV9038258.1"
                     /translation="MRKLILLLMAGLLASSILVRAQDKEVTGKVTDSTDNPVSGASIN
                     VKGVKRGTSAGPDGSFRLMAPVNGTIVVSAIGFLPQEVSVGSGNLTIQLRRDTRSLNE
                     VVVTALGIRREKRALTVATQTISSDEMNKSGTGNALGELEGKASGLTVIQSTGDPGGG
                     VYFRLRGSTSVTGNNQPLIIVDGVPLDNSINNFDATAPSGTISSGANGNLTGGQQPTN
                     RGNDLNPNDIESINVLKGPAATALYGIQAASGAIVITTKKGGAGRRTAVSFNSSVVWD
                     KVGLLPKLQDQWSQGSGGIYSPPEDQGSTSWGAKIDTLSWDGATNYAFDKHGNIVGKS
                     NPNAKIPVTPYDRYNFFKTGMTYNNNVSVSGGGENSGYRLSLGNLSQSGIVPRAQYNK
                     TTLSLSGQSKISSRLSTSAGLTFVHSTNYKVQQGSNLSGIMLGLLRTPVTFDNSDGFG
                     SDAFA"
     gene            1847..2914
                     /locus_tag="VGQ51_16605"
     CDS             1847..2914
                     /locus_tag="VGQ51_16605"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9038259.1"
                     /translation="MRIGLTAYPKPAMNKHHALNDRLVKTIGIPVLGIIVPNLTGLIT
                     NRLYSYSELTACYLFFIVVAFLVWEGNVRLMYFIRERFPWSRRSWYKIIVALFFANIV
                     YSGSLSFLLLRLWLFCSRETSAGEHLLNTVLVIIIAACFITNIYEILFLNLEKEYNES
                     RVEQLNVAKAQAELEALKNQIDPHFIFNSLNTLSFLITRDPANARLYNDTLARVYRYI
                     LSNKERDLVLLREEIDFISNYFYLLKIRFGDAVSMVIEITDLSAENFLIPPISLQALV
                     ENAIKHNEFSEKKPLTIDVSISADYVIVRNAMNRRNNPQPTSKIGLSNLDNRYKLLTK
                     RNIQVENNFEAFTVKLPVIRF"
     gene            3098..3847
                     /locus_tag="VGQ51_16610"
     CDS             3098..3847
                     /locus_tag="VGQ51_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV9038260.1"
                     /translation="MKVLIVEDERPASENLVEELLAIDENIDVVAGCNSVDETVRWLN
                     RNPQPDLILMDIQLSDGLSFNIFKACEVTCPVIFTTAYDKYLTQAFEYSSIDYLLKPI
                     SQDKLKNAIKKYKTLRSHFVNSQEPEYLNHNDRKRSRILVRKGVEFQAVRVEDAGYFF
                     TEHKLIFLVDKENRKYMAEKSNLSELEEELDRSIFYRANRKYIINANYIRRFKPLERS
                     KISVELTLPVNEEIIISQENSASFKKWIAQA"
     gene            complement(3914..5257)
                     /locus_tag="VGQ51_16615"
     CDS             complement(3914..5257)
                     /locus_tag="VGQ51_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV9038261.1"
                     /translation="MIFKRYQERILIRVLFMFLTLSVASFLLVKGMYEKLALVLPVII
                     AQLIDFYNFHRKAQQEVEQFVESIHYRDFSRYFDVKEAPLELQSLRQGFNEINSTFKL
                     VSREKETQYQYLQKILELVNTGILSYEHKSGEIGWMNEAFKSMLGVPYLKTINSLERR
                     DTNLYNEILNLRPGESKVVSLTKDKHIFKTLLAATAFQTDGRVYKLVAFQDVNEALDE
                     TEAKAWQKLLSVMTHEIMNSVAPISSLAETMLHRLQESARLLEGQAGALEDLELGIGT
                     IRRRSEGLLKFAETYRNLNKITTLNLKKVFARDLFENLHHLMLPTLEQKNIEMDILLM
                     DPDLALEADTNLIEQVLINLVVNAIEAVKDRDTPVIALAAYAGANGRPVIKVSDNGMG
                     MSPEVMDNIFIPFFSTKKTGSGIGLSLCKQILLLHKGNIQVQSAEGVGSAFLLHF"
     gene            complement(5271..>6158)
                     /locus_tag="VGQ51_16620"
     CDS             complement(5271..>6158)
                     /locus_tag="VGQ51_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV9038262.1"
                     /translation="FFKIKKIAPTDANILILGENGTGKDLIAKAIHQHSLRAEKPYIK
                     VDVGALTETLFESELFGHKKGAFTDAREDRVGRFEAGNGGTLFLDEIGNISLHQQAKL
                     LSVLQNRQVTRLGTNQAMPIDIRLICATNVPMSELANESRFRKDLIYRINTVEIVVPP
                     LRKREEDILLLARHFDRIYTNKYMKPLHDFDAKAMEKLRSYHYPGNVRELQYTIERAV
                     IMAEGDVLQAKDLIFSPIESATPAPDEPQEMDLKLSTIEKNAILKVIEKHSGNISKAA
                     KELGLTRTALYRRLSKYDI"
BASE COUNT         1538 a         1906 c         1252 g         1462 t
ORIGIN      
        1 gcgaatgcgt ccgacccaaa cccatcggag ttatcgaagg tcaccggtgt ccgcaaaagt
       61 cccagcatga tccctgacag gttggaccct tgttgaacct tatagttcgt tgagtggaca
      121 aatgttaagc ccgccgaagt gcttagccgg ctgctgatct tagactgccc gctcagactc
      181 agggttgtct tattatactg cgccctcggg acgatccccg actgcgaaag attcccgaga
      241 gataggcggt atccggaatt ttcaccgcca cccgacaccg agacattatt attataggtc
      301 atccctgtct tgaagaaatt ataccggtca taaggcgtca ccggtatctt ggcattcgga
      361 ttcgacttgc cgactatatt cccatgcttg tcaaaagcat aattcgtcgc tccatcccag
      421 gataaagtat cgatcttggc cccccaggat gtcgatccct ggtcctcagg cggggagtag
      481 ataccaccgg acccctgact ccactggtcc tgaagttttg gcaacaatcc cactttgtcc
      541 cacactaccg aagaattgaa gctcacggcg gtccgccttc ccgctccccc cttcttcgtc
      601 gtgatcacga tggcgccgct cgcagcctgt ataccataca atgccgtggc ggcgggcccc
      661 ttcagcacat tgatcgattc gatgtcattg gggttaaggt cattcccgcg gttcgtcggc
      721 tgctgacccc cggtcagatt accattagcg ccactggaaa tggttcccga cggagcggta
      781 gcgtcaaaat tattgatcga gttgtcgagc ggtaccccgt cgacgatgat caacggctgg
      841 ttgttacccg tcacggaagt ggaaccccgc aatcggaaat aaaccccgcc gccgggatcg
      901 cccgtactct ggataaccgt tagtcccgaa gccttgcctt ccagctctcc aagcgcattg
      961 ccggtccccg acttattcat ctcatcggag gaaatggtct gagtcgccac cgtcagcgct
     1021 ctcttttctc ttcggatgcc gagcgcggtc acgaccacct cgttcaggct cctcgtgtcc
     1081 cgccggagct gtattgtaag attaccggaa cccacggaaa cttcctgcgg caggaatcct
     1141 atcgcactca ccacaatcgt cccattgact ggggccataa gccggaaact cccgtcaggg
     1201 cccgcgctgg tcccccgttt cacgcctttg acattgatgg atgcccccga aaccgggtta
     1261 tccgtagagt cagtcacctt tcccgtcacc tccttatcct gtgcacgcac aaggatagag
     1321 gatgccagta gccccgccat gagcaaaaga ataagctttc tcatttgctg tttggtttag
     1381 aatagtgttg aaaatgtagg ttaactaagg gcgcacgcag agcgccgaaa aaaaaagaca
     1441 cataaattaa gggatgtgac gcaccgcggg gagcgatact tacccaagca ggaaagaagc
     1501 cgaccaaaac ccgcaatcga aagcctgaat cggcataaaa aaagcaggtg gtagaaacca
     1561 cctgcgggtc acatgagaaa actttgtcgc gctgcgtgag cggcgcgacg gcgaaagata
     1621 attacatttt atagtcgtag cggcgtaaac tgactcaagc aggagatttt agggccaaac
     1681 gggttattta acggtatgaa tgccgccgat caattctttt tgccgcaata tccatgtctt
     1741 gctccttttg caataaatta acttttctaa tacattacat ttaagaaaat tgccgttatt
     1801 cccttctact acaccagcga agtattttcc cgaaacacta aaaattatgc gcataggcct
     1861 cacagcctat cccaaacctg ctatgaacaa acatcacgct ttaaacgatc ggttggtcaa
     1921 gaccatcggc atccctgtat taggtatcat cgtacctaac ctcacaggac tgatcaccaa
     1981 ccgtctttac agctactccg agctgactgc ctgttatctc ttctttatcg tagtcgcctt
     2041 cctcgtctgg gaaggcaatg tgcgcctcat gtacttcatc cgcgaaagat tcccctggtc
     2101 caggagatca tggtataaga tcatcgtcgc tctcttcttc gccaatatcg tctactccgg
     2161 atccctctcc ttcctgctgc tgcgcctttg gctcttctgc tcccgcgaaa catcggctgg
     2221 cgaacacctg ctcaataccg tcctggtcat catcatcgcc gcctgcttca tcaccaacat
     2281 ctatgagata ctctttctca atctggaaaa ggaatacaac gaatcccgcg tcgaacagct
     2341 caacgtcgcc aaagcacagg cggaactcga agccctcaaa aaccagatcg acccccactt
     2401 catcttcaat tcgctcaata ccctttcctt cctgatcacc cgcgaccccg ccaacgcccg
     2461 gctgtataat gataccctcg cccgggtcta tcggtatatc ctaagtaata aagaacgcga
     2521 cctggtcctc ctccgggaag agatagattt tattagcaat tacttctacc tgctgaagat
     2581 ccgcttcggc gatgccgtca gcatggtcat cgaaataacc gacctgtccg ccgaaaattt
     2641 ccttatcccg ccgatctctc tccaggccct cgtcgaaaac gccataaaac acaacgaatt
     2701 cagtgaaaaa aaacccctga ccatagatgt ctccatttcg gccgactatg tcatcgtccg
     2761 caacgctatg aaccgacgca acaatccgca acccacttcc aagatcggcc tgagtaacct
     2821 tgacaaccgc tacaaactgc taaccaaacg caatatccag gtggaaaata atttcgaagc
     2881 atttaccgtc aaactgccgg tcattcgctt ctgatttcaa ccgttcaccc taacgattca
     2941 accattccca gattccgcac cctcgttctc gcaaacccta ccctattatt caaaatttca
     3001 tgcaacattt tccgtgcaaa cgcgtcatat aaactgtacg aacggtaacc tatccacaga
     3061 agaactaact aattttacaa caacaaaact cagccgaatg aaagtcctta tcgtggaaga
     3121 tgaacggccc gcctccgaga atctggtcga agagctcctg gccatagatg aaaatataga
     3181 cgtggtagca ggctgcaaca gcgtcgatga gacagtcagg tggcttaacc gcaatcccca
     3241 acccgatctt atcctcatgg acatccaatt gtccgacggc ctttcgttca atatattcaa
     3301 agcctgcgaa gtcacctgcc cggtcatctt taccaccgcc tatgataaat acctcaccca
     3361 ggcattcgaa tacagtagca tcgattacct gctaaaacct attagccagg acaaactgaa
     3421 aaacgccatc aaaaaataca agaccctccg gagtcatttt gtcaacagcc aggagcccga
     3481 atatctcaat cataacgacc gcaaaagatc ccgcatcctc gtccggaaag gagtcgaatt
     3541 ccaggccgtc cgcgtcgaag acgccggcta cttcttcacc gaacacaaac tcatttttct
     3601 ggtagataaa gaaaaccgga aatacatggc ggagaaaagc aatctcagcg agctggaaga
     3661 agaactcgac cgatccatct tctaccgcgc caatcgcaaa tacatcatca acgcgaatta
     3721 tatccgtcgg ttcaaaccgc tcgaacgtag caagatcagc gtcgagctca ccctcccggt
     3781 caatgaagag atcatcatca gccaggagaa ttccgcctca ttcaaaaaat ggatcgcaca
     3841 ggcctgaacc ccggcatcgc ccaatcccgg catcgccgac cccggcatcg ccaaccccgg
     3901 catcgccggc ccatcaaaaa tgcagcagga acgcgcttcc taccccttct gccgactgca
     3961 cctgtatatt ccccttatgg agtaagagta tctgcttaca taaactcaac cctatcccgc
     4021 tccccgtctt cttcgtacta aaaaaaggta tgaaaatatt gtccatgacc tcaggcgaca
     4081 tccccatccc attgtccgat accttaatga ccggccgccc attcgccccc gcatatgccg
     4141 ccaacgcgat caccggcgta tccctatcct tcaccgcctc tatagcattc acaacgagat
     4201 tgatcaatac ctgctcgata agattcgtat ccgcctccaa cgccagatcg ggatccataa
     4261 gcaaaatgtc catctcgata ttcttctgtt ccagcgtcgg cagcatcagg tgatgcaggt
     4321 tctcgaaaag atccctggcg aacactttct taagattcag cgtagttatc ttattgagat
     4381 ttcgataggt ctcagcgaat ttcaacaacc cctcacttcg ccttcggatc gtaccgatcc
     4441 ccagctcgag atcttccaac gcccccgcct gcccctccag taatctcgcg gactcctgca
     4501 gccggtgcaa catcgtctcc gccagactcg agataggagc caccgaattc atgatctcat
     4561 gcgtcatcac actcaataac ttctgccagg ccttcgcctc ggtctcatcc aacgcctcat
     4621 tgacatcctg aaatgccacc agcttatata cgcgtccatc cgtctggaac gccgtggcgg
     4681 ccagcagcgt cttgaagata tgcttgtcct tcgtcagcga gacgacctta ctctcccccg
     4741 gccgcaggtt caggatctca ttatacaggt tcgtatccct ccgctccaaa gagttgatcg
     4801 ttttcagata cgggaccccc agcatactct tgaaggcttc attcatccag ccgatctctc
     4861 cactcttatg ttcatacgaa aggatccccg tattcaccag ctcgagtatc ttctgcagat
     4921 actggtactg cgtctccttc tccctgctca ccagcttgaa cgtcgaattg atctcattga
     4981 atccctgccg caaggattgc agctccagcg gcgcctcctt gacatcgaaa taccgcgaga
     5041 aatcccggta atggatggac tccacgaatt gctccacttc ctgctgcgcc ttgcgatgaa
     5101 aattgtagaa atcgatcagc tgcgcgatga tcaccggcaa tacgagcgcc agcttctcat
     5161 acatcccttt caccaggagg aaggaggcaa ccgacaacgt caggaacata aagagcaccc
     5221 gtataagtat cctctcctga tatcttttaa agatcatcct aaaaaaataa ttagatgtcg
     5281 tatttactca accgcctgta caacgccgtc ctcgtcaaac ccaattcctt cgcggccttc
     5341 gaaatattcc cactatgctt ctctatcacc ttcaatatcg cattcttttc tatcgtactc
     5401 aatttcagat ccatttcctg cggctcatcc ggcgccggag tagccgactc tataggcgag
     5461 aagatcagat ccttcgcctg caacacatcc ccctccgcca tgatcaccgc ccgctcgatc
     5521 gtatactgca gctcccggac attacccgga taatgatacg atcgcagctt ttccatcgcc
     5581 tttgcatcga aatcatgcag cggtttcata tacttgttcg tatatatcct gtcgaaatgc
     5641 cgcgccagga gcaggatgtc ctcctccctc ttcctcaacg gcggcaccac gatctctacc
     5701 gtgttgatcc ggtagatgag gtccttccgg aaccggctct cgttcgccag ctcgctcatc
     5761 ggcacattcg tcgcacagat cagcctgata tcgataggca tcgcctggtt cgtccccagt
     5821 ctcgtcacct gccggttctg caacacactc aacagcttcg cctgctggtg taatgatata
     5881 ttcccgatct catccaggaa cagcgtcccc ccgttacccg cttcaaaacg ccctaccctg
     5941 tcctcgcgcg catccgtaaa ggcccccttc ttatgtccaa acaattcact ctcgaataag
     6001 gtttccgtca atgcccccac gtccactttg atataaggct tctccgccct caacgaatgc
     6061 tgatgaatgg ccttggcgat caagtcttta cccgttccat tctcccctag gatcaggatg
     6121 ttcgcatccg taggcgctat cttctttatt ttgaaaaa
//
LOCUS       DASYSV010000532        15843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_365467, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000532 DASYSV010000000
VERSION     DASYSV010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 15843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15843
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365467"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VGQ51_16625"
     CDS             complement(<1..481)
                     /locus_tag="VGQ51_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEV9038263.1"
                     /translation="MRKLLFVLLFSAFVLAGRTQGTPQDSAWIRENYYKLETYIPMRD
                     GVKLFTSMYIPKDTTEQHPILMTRTPYSCAPYGGDKWRNYYTSYLRYYLHKGYIMVTQ
                     DVRGRWMSEGEFVDVRPFIKDKKSKTDVDEASDTYDAIDWLVKNVPHNNGRVGIFGIS
                     "
     gene            578..1540
                     /locus_tag="VGQ51_16630"
     CDS             578..1540
                     /locus_tag="VGQ51_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEV9038264.1"
                     /translation="MLAASACRSREDGSTVAVQSADTIPPVAVTGQPVVLSTWNEGAA
                     ANKGAWEVLGHGGNALDAVEAGVKITESSRNCCVGLGANPDRDGHVTLDASIMDHQWN
                     CGAVAALERIKHPISVARRVMEKTPHVLLVGVGAQQFAVAEGFPLEADVLSPEAKHAY
                     EEWLKKSEYKPVINIENHDTIGMIAMDTTGNLSGSCTTSGMAFKMRGRVGDSPIIGAG
                     LFVDNEVGAVTATGQGEDVIRMCGSHSVVEYMRQGMSPEQACKTAVEKIARIKGAKAK
                     EIQVAFIAINKKGDTGGYALQPGFSYAIKSNSEERMIVSKSHFA"
     gene            1575..2309
                     /locus_tag="VGQ51_16635"
     CDS             1575..2309
                     /locus_tag="VGQ51_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV9038265.1"
                     /translation="MNYRLEICAFNLASAMIAQQAGADRVELCAGPEEGGTTPSPATI
                     ALAREKLRIPLYPIIRPRGGDFLYSDDEFRVMLRDVEFCKKAGCNGVVFGLLAADGTI
                     DTARCARLVEMAYPLGVTFHRAFDWASNPFQAMETIIGLGCERILTSGQRPTAEEGAE
                     LIDQLIREADERIVIMPGSGVRASNIVELAEKTGASEFHTSARVLKASAMTYVNAGMK
                     ENQSVVLAGEEEIKKIREQLTAISAS"
     gene            complement(2293..3786)
                     /gene="pyk"
                     /locus_tag="VGQ51_16640"
     CDS             complement(2293..3786)
                     /gene="pyk"
                     /locus_tag="VGQ51_16640"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV9038266.1"
                     /translation="MSKNLSKYLYQDMDKEAGLQHTFHKTKIVATVGPACDTYEKLLD
                     LVKAGVNVFRLNFSHGAHEDKKKIIEHIRNINLTEPYNISILGDLQGPKLRVGDIKDG
                     SLTIAPGDILTFTSREKVVGTKEKIYVSYPNLHHDVEVGNKILIDDGKLEVVVVNVTP
                     EGDVKVAVTYGGVLLPKKGVNLPDTKISLPAMTEKDIVDLEFIIEQKLDWVALSFVRK
                     VEDIIDLKRRLAEKKCQSKVIAKIEMPSAMEDLRNIVLESDAVMVARGDLGVELPVEK
                     VPMAQREIIRKCIHRAKPVIVATQMMESMIDRVKPNRSEITDVANAVLEGADAVMLSA
                     ETATGQHPALVVETMRKIILEVERTEYPYNREDDLVPQPHSPSFLSDAICYNACKLAK
                     DANADALVGMTQSGYTAFILSSYRPKSPLYIFSKEKSLINQLSLSWGVRAFHYAEEES
                     VDDIIHDQHVILKERGFVKPGDVIVNTCSLPIQEHLPTNMLKITKLK"
     gene            complement(3886..4863)
                     /gene="pfkA"
                     /locus_tag="VGQ51_16645"
     CDS             complement(3886..4863)
                     /gene="pfkA"
                     /locus_tag="VGQ51_16645"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV9038267.1"
                     /translation="MENKVTKIGVLTSGGDAPGMNAAIRAVVRTGIYNGMEVFGIMRG
                     YSGMIENDIVPMHSRSVANIIQRGGTILKTARCKEFFEFDGRKKAYNNLKKLGINGLV
                     IIGGDGSFRGAHKLSEEFDIPCIGLPGTIDKDIAGTDFTIGFDTAVNTAVEAIDKIRD
                     TADAHDRLFIIEVMGRDAGYIALHSGIATGAENILIPERKTDIDELVNSLLEKERRKK
                     LVNIIVVAEGDRGAEDVAAIIKERVPTADTRVCVLGHIQRGGAPSCLDRLIASRMGYS
                     AVECLIEGRHNVMVGIVNNKMHYTPLEKAVKAKQKISEDWLKIVKILAS"
     gene            5072..5689
                     /locus_tag="VGQ51_16650"
     CDS             5072..5689
                     /locus_tag="VGQ51_16650"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEV9038268.1"
                     /translation="MPPDDPETAALWPVISSRMPDGGSKGPLRVYVGGTEWGRSSWVG
                     KMYPVGTKSADYLKMYARQLDTVELNTLFYGLQPPAVLQRWADAVGSGFRFCPKFPET
                     ISHKGQLSGVGKDTDEFIERVGLLGSRLGPSFLQSSERFGPERAVVLRDYVRRMPPGF
                     RVAVELRHEGWFGGGSAALGEVWQLLCSGGRLGRAHSGMGSKGAA"
     gene            complement(5757..6671)
                     /locus_tag="VGQ51_16655"
     CDS             complement(5757..6671)
                     /locus_tag="VGQ51_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEV9038269.1"
                     /translation="MQTTAYLLMIRPVNFSFNAETAVNNAFQIPGIAESPQRQALSEF
                     EGFLKLLRDNEVDVIVIDDTPEPYTPDSIFPNNWVSFHDDGSVCLYPMYAVNRRQERK
                     PDVLKTVGSQFRIGDIQDLSGYERDGLFLEGTGSMVLDREAKIAYACYSPRTNAIVLR
                     EFCKRLGYTEVTFKAVDAFGRDIYHTNVMMCVADRYVVVCLDALPHDAERKHLEDTIL
                     SSGKTIVPITIDQVNHFAGNMLQVHNRKGEKLLVMSSQAYESLDAEQRRVLTSFNRIL
                     HAPLTTIEANGGGSARCMLAEIHLPPLK"
     gene            complement(6700..8160)
                     /locus_tag="VGQ51_16660"
     CDS             complement(6700..8160)
                     /locus_tag="VGQ51_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEV9038270.1"
                     /translation="MSKHYPHKNRVAVSVSSEVGRLRRVLVHSPDSGLGKVVPSKAQD
                     WLFEDIVHLDTIRRKEYDYYTKILLYFLDPGKIKGRLAVVDDEKNRRAFYKPGDEGFF
                     RSDKVVELQWLLADILADNAIKLKLVASVCAIEACSYDIQQGLQELDPVQLAKTFISG
                     ATDDDRLLFAPIPNFIFTRDIGIVINDHVLLNKPAKKARTREALLAKYIFFNHPLFEE
                     YRDRIIELNDTHHHFLLPREGDERKVTLEGGDVMVICKDHLLIGISERTSAEAAHQAI
                     RVLFERNVVKKITIIRIPKKRDFMHIDTIFTQIKRNVWILLGTFSSKSIKHEDEDDVQ
                     RILDNAKKEDKLKIIQFRKKDQSNPEYFDSLEDLLTDISRNDLECTDKVKFIYSGNNE
                     FPFDAREQWTDSCNLLALKEGVVLGYDRNDKTVEAFKANGFDVISAEDLVSKMEKDEI
                     SPADVENTLILMPSAELSRARGGFHCMSMPLHRDDL"
     gene            8227..8733
                     /locus_tag="VGQ51_16665"
     CDS             8227..8733
                     /locus_tag="VGQ51_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038271.1"
                     /translation="MELITDQTIDLINDRIQRMDEGQIGRIWDSFAKEQPALVGYVLG
                     ESRELEEPDAREDVAYVLTIILLSFQELTPGIPAVTEQEFEKAFNEEFDEMESVFQDG
                     FDESDAMAVIESFCQPDLLQFAAATIYSEDADEEDASNFTEEEAGLFIVIFKTAIALL
                     HKKAGSII"
     gene            8844..9998
                     /locus_tag="VGQ51_16670"
     CDS             8844..9998
                     /locus_tag="VGQ51_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV9038272.1"
                     /translation="MHFQLSEEHLMIRKAARDFARNECLPGVIERDEHQKFPKEQVMR
                     LADLGFMGMMVDPAYGGSGMDTVSYVLAMEEISKIDASVSVCMSVNNSLVCWGLDTYA
                     NQAQKERYLTPLAQGKRDGELYIGAFLLSEPEAGSDATSQRTIAEEKGDHYLLNGVKN
                     WITNGNSASVYLVMAQTDISKGSHGINAFIVEKSWPGVAVGGKENKLGIRGSDTHSIS
                     FSDVKVPKENRIGADGFGFTFAMKTLAGGRIGIASQALGIASGAYELAVNYSKERKAF
                     GAPIAQHQAIQFKLADMATRIEAARLLCLRAAWEKDSNLDYALSSSMAKVYASETAMW
                     TATEAVQVHGGYGYVKEYHVERLMRDAKITQIYEGTSEVQRIVISRSILK"
     gene            complement(10113..12512)
                     /locus_tag="VGQ51_16675"
     CDS             complement(10113..12512)
                     /locus_tag="VGQ51_16675"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HEV9038273.1"
                     /translation="MKHYTYTLLFLLPLFAVAQTKPHAGSSVLSGKIIDGKTKQPIPT
                     ATITLLRKDSSVAARVISQSDGEFALKNLPESPSILEVSVVGYEPIYRQIAGGHHAPG
                     AIVNAGTIRLTSTASEMTTAVVVAQRPIFKTEIDKKVFNVDQSLASKGGTAQDALRQV
                     PTLNVDASGNVTLRNGSPTILLDGKQTDLTLDQIPADQIQSIEVMPNPSAKYDAQGNN
                     GIVNIVLKKNRKPGMNGSFTGVGSTLGETYGFANMNVYKHKWNFRLNYMGHTHRSVSN
                     TTTTFDNLAANTTAIQHGHAVTTGPFQNLRLGADYNMDEHNTFSLSGNIGFGSHPTRG
                     TQNTAYWEKDALLDSNGFRNSYAGNQFVFSHAHFDYGHTFKKEDEKLSMSAAAETYHG
                     KNYGNYDMQFLNKGGSTLASPNVQRYNGHGNGYHVTMQGDYSDPLMDGKSKLDVGVKT
                     ILRGNRSFQDFEDSSAQGYVRDPNASYNYSYDNNTYAAYGAFTQQLGKFSYMAGLRYE
                     KYTYTGHLLDSNTSFGYQESGLYPSVFLTEKFDDDNDLHLNFSRRINRPQWWQITPQT
                     NYGNPLNPQRGNPGIRPENTNLLELAYNTQLSGVALSTTVYLKNTLDPMMSYNKPLSN
                     DTLLSTFENGNYANTYGAEIIARIPILKWWNATTNFNLFQTEINANNLSQGLSNSGFS
                     WFAKLNSDMKLWNTFTLQMTGSYSAASYFAQGKTLPSGGLDLAVKRDILRHKAGTLVL
                     SLSDVFNTERSRTDTYSPGVFYQDAISKPETRVLKLSFTYSFGKELNGDRHKADDSTN
                     G"
     gene            12602..13237
                     /locus_tag="VGQ51_16680"
     CDS             12602..13237
                     /locus_tag="VGQ51_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV9038274.1"
                     /translation="MSADKDVYRQILSELGENVQLVAVSKTKTAEDILALYAEGQRDF
                     GENYVQELADKQSRLPTDIRWHFIGHLQTNKVKQIVPFVHLIEGVDSLRLLNEIEKQA
                     VRAGVVIDVLLQVHIAREETKFGFGAEEIGSAMGVHRDGVRIRGLMGMASNTSDPDAV
                     RSEFRVLKGLFDRWFPDGGVLSMGMSADYKTAIEEGSTMVRIGSLLFGRRG"
     gene            13253..14932
                     /locus_tag="VGQ51_16685"
     CDS             13253..14932
                     /locus_tag="VGQ51_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEV9038275.1"
                     /translation="MDEIKKEEEKSLNFLEEIVVEDLAAGKYKSILTRFPPEPNGYLH
                     IGHSKSIVLNFGLAQKYGGKTNLRFDDTNPITEDVEYVDSIKEDIRWLGFEWAGEFYA
                     SDYFEQLYQFAENLILKGLAYVDDSSAEEIAKMKGTPTEAGTDSPYRSRSVEENLRLF
                     REMRAGKFRDGEKVLRAKIDMASPNMHFRDPFMYRIRHAHHHRTGDAWCIYPMYDFAH
                     GQSDSIEHITHSVCTLEFIPHRPLYDWFIEKLEIFPSHQYEFARLNLNYTVMSKRKLL
                     QLVNEGHVTGWDDPRMPTISGLRRRGYTPESIRMFAEKVGVARRENLIDVSLLEFCVR
                     EHLNKIAFRRMVVFDPLKIVLTNYTGDEELLSSEDNPEDASTTHRDIPFSRELYIERE
                     DFMENPPKKFFRLSPGQKVRLKSAYIIQCDEVIKDTEGRVIGLRCSYLPESKSGGAQS
                     NMKVQATIHWVSVPHAITAEVKLYDRLFRVENPAGEEGDFKDYVNPDSLQVLPGAFAE
                     PALRGAKFDEHYQFLRKGYFYLDKDSSEDKMVFNRTATLRDTWEKEKKKSG"
     gene            complement(14957..15400)
                     /locus_tag="VGQ51_16690"
     CDS             complement(14957..15400)
                     /locus_tag="VGQ51_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038276.1"
                     /translation="MNFRKSNDGPSANGRGVRLLIACATLVTSLLFVSSTFAQGGGMR
                     GDTAAMRQRMEAQLAKMKTDLNLTQTQCDSVKAIQMDQRAAMRQIFMDPANQGLSRED
                     RMAKMQPINEAMIARLNAALGADLSKKVQDWMQANRPQRGGGGNR"
     gene            15643..>15843
                     /locus_tag="VGQ51_16695"
     CDS             15643..>15843
                     /locus_tag="VGQ51_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038277.1"
                     /translation="MYLPIITGYELGQFVRILMWIFVPVAILSMLVTTWLHYRRRRRN
                     SEEGLLSIDGFGGEGFVMPLQVS"
BASE COUNT         3474 a         3740 c         4771 g         3858 t
ORIGIN      
        1 aggagatgcc gaagataccc acgcgtccgt tgttatgagg aacgttcttt accagccagt
       61 cgatcgcgtc ataggtatcg ctggcctcgt cgacgtcggt tttcgatttt ttgtccttga
      121 tgaaaggccg cacgtctacg aattctcctt cgctcatcca gcggccgcgt acatcctggg
      181 tgaccatgat atagccttta tgcagatagt agcggagata gctggtataa taattcctcc
      241 acttgtcacc tccataaggg gcgcacgagt agggagtgcg ggtcatcagg atgggatgct
      301 gttctgtcgt atctttgggg atatacatgg aggtgaagag ctttaccccg tcccgcatcg
      361 ggatataggt ctccaactta taataatttt ctcttatcca agcgctgtcc tggggagtgc
      421 cttgcgtacg tcctgcaagg acgaaggccg aaaagagcag tacgaagagg agttttctca
      481 tgttaatcgt taattttaat ttgccgaacc aatttaagta atcatgtcca accgccgaaa
      541 atttttgcaa tcctcgttat taagttcatt tggtgtaatg ctcgccgcgt cagcctgtcg
      601 gtcccgtgag gatgggtcta ccgttgccgt tcagtccgca gatacgatcc caccggtggc
      661 ggttaccgga cagccggtgg tgctttcgac ctggaatgag ggtgcggcgg ccaataaggg
      721 cgcctgggag gtgctggggc acggcgggaa cgcgctggat gcggtggaag ccggggtgaa
      781 gatcacagaa tcttcgcgca actgctgtgt gggtcttggc gccaatcccg accgtgacgg
      841 gcatgtcacg ctggacgcga gcatcatgga tcaccagtgg aattgcggtg cagtggcggc
      901 gctggaaagg atcaaacatc ccatttcggt ggcccgccgc gtcatggaga agacgccgca
      961 tgtgttgctg gtcggcgtgg gggcgcaaca gtttgccgtg gccgaaggtt ttccgctgga
     1021 ggcggatgta ctgagccccg aggcaaaaca tgcctatgag gaatggttga aaaaatcgga
     1081 atataaaccc gtgatcaata tcgaaaacca cgacactatc gggatgatcg cgatggatac
     1141 gaccgggaat cttagcggca gctgtacgac aagcggtatg gcgttcaaga tgcgcggcag
     1201 ggtgggcgat tcgcctatca tcggggcggg gttattcgtt gataacgaag tgggcgccgt
     1261 gacggctacg ggtcaggggg aggatgtgat ccggatgtgc ggatcgcatt cggtggtgga
     1321 atatatgcgc cagggcatgt cgcccgagca ggcctgtaag accgccgtgg agaagatagc
     1381 gcggatcaag ggggcaaaag ccaaagagat acaggtggcg ttcatcgcca tcaataagaa
     1441 aggggacacc gggggatatg cgttgcagcc gggattctcc tatgcgataa agagtaattc
     1501 tgaggagagg atgatcgtgt cgaaaagcca ttttgcatga acgcgccggg aaccggcgca
     1561 atgagggggg aattatgaac taccggttgg agatatgcgc atttaatctg gcatccgcaa
     1621 tgatcgcgca gcaggcgggg gcggatagag tagagctatg cgccgggcct gaagaaggcg
     1681 gaaccacgcc ttctcccgcc acgatagctt tggcgaggga gaagctgcgc attccattgt
     1741 atcccattat ccggccccgt ggcggagact tcctgtatag cgatgatgaa ttccgggtca
     1801 tgctacgcga cgtcgaattc tgtaagaagg caggttgcaa tggggtagtc tttggcttat
     1861 tggctgcgga tggaacgatc gacacggcgc gatgtgcacg actggtggag atggcctatc
     1921 cgctgggcgt gacctttcac cgggctttcg actgggcgtc caatcctttt caggctatgg
     1981 agacgatcat aggacttggt tgtgagagaa ttctgacctc cgggcagcga cctacggccg
     2041 aagaaggcgc ggagttgatc gatcaactta tccgggaggc cgatgaacgt attgtcatca
     2101 tgcccggatc gggcgtgcgg gcatcgaata tcgttgaact ggccgaaaaa acgggtgcct
     2161 ccgaattcca tacctcggca agggtcctga aggccagcgc catgacgtat gtcaatgcgg
     2221 ggatgaaaga gaaccagtcg gtggtcctcg ccggtgaaga agagatcaaa aagataaggg
     2281 agcagctgac ggctatttca gcttcgtgat cttgagcata ttggtcggca gatgttcctg
     2341 gatcggcaga ctgcaggtat tgacgatgac atctcccggt tttacgaagc ctctttcctt
     2401 caggatcacg tgctgatcgt ggataatatc gtccacgctt tcctcttccg cataatgaaa
     2461 ggcgcgtact ccccagctaa ggctcagctg gttgatcagg cttttttcct tcgaaaagat
     2521 gtatagcggc gattttggcc ggtagctgga caggatgaag gccgtgtagc cgctttgcgt
     2581 catcccgacg agggcgtccg cgttggcgtc cttggccagt ttgcacgcat tgtagcagat
     2641 ggcgtcgctg agaaaggagg gcgaatgcgg ctgagggacg aggtcatctt cacggttgta
     2701 gggatattcg gtcctttcga cttcgaggat gatctttcgc atcgtctcca cgacgagtgc
     2761 cggatgctga ccggtggccg tttcggcgct gagcatgacg gcatccgcgc cttccagcac
     2821 ggcattggct acgtcggtga tctcgctgcg gttgggcttg acgcgatcga tcatgctttc
     2881 catcatctga gtggcgacga tgaccggttt tgcgcggtgg atacatttcc ggatgatctc
     2941 gcgttgcgcc attggtactt tttcgacggg cagttcgaca cccagatcgc cgcgggcgac
     3001 catgacggcg tcggattcca gaacgatatt gcggaggtct tccatcgccg aaggcatttc
     3061 gatcttggcg atgaccttgg attggcattt cttctccgcc aggcggcgtt tgagatcgat
     3121 gatgtcttcg accttgcgga cgaacgataa cgccacccaa tccagttttt gttcgatgat
     3181 gaattccagg tcgacgatgt ctttttctgt catcgcggga agcgagatct tggtatcggg
     3241 aaggttgacg ccttttttcg gaaggagcac gccgccatag gtgacggcga cttttacgtc
     3301 cccctcggga gtgacgttaa ccaccactac ttccagcttt ccgtcgtcga tgagtatctt
     3361 gttgcctacc tcaacgtcat ggtgaagatt gggataggaa acgtagatct tttccttggt
     3421 gccgacgacc ttttcgcgcg aggtgaaagt caggatgtca ccgggtgcga tcgtgagact
     3481 gccgtccttg atgtcgccaa cgcgaagctt ggggccctgc agatcgccta gtatggaaat
     3541 attatagggt tcggttagat ttatattgcg gatatgttcg atgatcttct ttttgtcctc
     3601 atgcgcgccg tgtgagaaat tgagacggaa gacattgacg cccgccttga cgagatcgag
     3661 cagcttttca taagtgtcgc aggcgggtcc gactgtggct acgatcttgg tcttatggaa
     3721 cgtatgctgc agaccagctt ctttatccat atcctggtag aggtacttcg ataaattctt
     3781 tgacattgct actgttttag tgccggagat tcgcctttcc aaaaactagc gcctctccgg
     3841 cacatgcgct aatgattgtc cttcggacga ggcccttcca gggcctcagg atgctaatat
     3901 ttttacgatc ttcagccagt cttcactgat cttctgtttg gcttttacgg ccttctcgag
     3961 cggggtgtag tgcatcttat tgttcactat accgaccatc acgttgtgac ggccctcgat
     4021 gagacattcg acggcggaat agcccatgcg gcttgcgatg agacgatcca aacaggacgg
     4081 tgctccgccg cgttgaatat gtccgaggac gcagacccgg gtatccgctg ttggtacgcg
     4141 ttccttgatg atggcggcca catcttcggc gccacggtcg ccttccgcga cgacgatgat
     4201 attgaccagc tttttacggc gctctttttc gagcagggaa ttaaccagct catcgatatc
     4261 ggtcttgcgt tcggggatga ggatgttctc ggcgccggtg gcgataccgc tatgcagtgc
     4321 gatatagcct gcatcgcggc ccattacttc tataataaaa agacgatcgt gggcgtcggc
     4381 ggtgtcgcgg atcttgtcga tggcttccac ggcggtgttg acggccgtgt cgaaaccgat
     4441 ggtgaaatcg gtgccggcga tatctttgtc gatggtgcca gggagaccga tgcaggggat
     4501 gtcgaactcc tcgctgagct tgtgcgcacc acggaacgag ccgtccccac cgatgattac
     4561 taagccgttg atacccagtt ttttgagatt attgtatgcc ttttttcggc catcgaattc
     4621 gaaaaattct ttgcagcggg ccgtcttaag aatggttccc ccgcgctgga tgatattggc
     4681 aacgctgcgg gagtgcatgg gtacgatatc gttctcgatc atcccgctat atccccgcat
     4741 gataccaaat acttccattc cattataaat accggtacga acaacggcac ggatggctgc
     4801 attcatcccc ggtgcatcgc cgccggaagt gagtacgcca attttagtga ctttgttttc
     4861 cattttggaa ccctgttctt gtaattgaat tttaaaaagc tttaaccaat ggcatgaatt
     4921 gttgaaaaat ttctacctta aacccccgtc tatacgggca ggagggcagt cgaagtccca
     4981 aaagtaagca attgaattga atcccatgca ataatggagc tgtatcgtgg aatttgggaa
     5041 agtacaatat atagataaca ttgatttttc attgcctcct gatgatccgg agacggcggc
     5101 gttatggccg gtaatatcct cgcggatgcc cgatggaggc tcgaaagggc ctctgcgggt
     5161 ttatgtagga ggcacggagt gggggcgaag cagctgggtc ggcaagatgt atccggtggg
     5221 aacaaagagc gcggattatt tgaaaatgta tgcgcggcag ctggatacgg tcgagctgaa
     5281 tacgctgttc tatggattgc agccgccggc cgttcttcag cgttgggccg atgccgttgg
     5341 gtccggattc cgattttgtc cgaaatttcc cgagacgatc agtcataaag gtcaactctc
     5401 gggcgtgggg aaggacacgg atgaatttat tgaaagggtc gggttgttgg gttcccggtt
     5461 ggggccttcg tttttacagt cgtccgagcg gtttggtccg gaacgtgcgg tagtgttgcg
     5521 ggattatgtg cgccggatgc cgcctggttt tcgggtggcg gtagagttgc ggcatgaggg
     5581 atggtttggc ggtggttcgg cagcgttagg tgaggtatgg caactgttat gttcgggcgg
     5641 acgcctgggt cgagcgcata gcgggatggg cagcaaaggg gctgcgtgaa gtatattgtg
     5701 gtacggggct gcgggttccg caattggtca atggcggaca gccggggaat ctctccttat
     5761 tttaacgggg gcagatgtat ttctgcgagc atgcagcgtg cgctgcctcc gccgttggct
     5821 tcgatggtcg tcaggggcgc gtggaggata cggttgaagg aagtgaggac ccgacgttgc
     5881 tccgcgtcga gcgattcgta ggcctgcgaa gacatgacca gcagtttttc tcctttccgg
     5941 ttatgcacct gcagcatatt gcccgcgaaa tgattcactt gatcgatagt gatgggtacg
     6001 atcgttttac cggaggagag gatggtatct tcgagatgtt tgcgttcggc atcgtggggc
     6061 agcgcatcga gacagacgac tacataccgg tccgccacgc acatcatgac attggtatga
     6121 tagatgtccc gtccgaaggc gtccactgcc ttaaaggtca cctcggtgta tcccagccgt
     6181 ttgcaaaact cgcggagcac gatggcgttg gtgcgcgggg aatagcaggc gtaggcgatc
     6241 ttggcctcgc gatcgaggac catgctgccg gtcccttcga ggaagagtcc gtcgcgttcg
     6301 tatccgctga ggtcctggat atcgccgatg cggaattgac tcccgacggt cttaaggaca
     6361 tccggcttcc gttcctgccg gcgattgacg gcatacatcg ggtagagaca gacggagccg
     6421 tcgtcgtgaa aggagaccca attgttgggg aagatgctgt cgggcgtata aggctcggga
     6481 gtgtcgtcga taacgatgac gtctacctca ttgtcacgca ataatttcag gaaaccttcg
     6541 aattcggaga gtgcttgcct ttgcgggctc tcggcgatgc cggggatttg gaaggcgtta
     6601 ttgactgcgg tctcggcgtt gaagctgaaa ttgactggcc gaatcatcaa taaataggcg
     6661 gtcgtttgca tgaagcatgg tttttgcata tggattgtat tagagatcgt ctcgatgcaa
     6721 gggcatactc atgcaatgaa aaccgccacg ggcccgggat aattcagccg aaggcatgag
     6781 gatcaacgta ttttcgacat cggcgggact gatctcgtct ttttccatct tcgaaaccag
     6841 gtcctctgcg gaaattacgt caaagccgtt ggctttgaaa gcctccacag tcttgtcatt
     6901 ccggtcatag cccaacacga cgccttcctt caaagctaat aagttacaag aatcggtcca
     6961 ttgttcgcgt gcgtcgaaag gaaattcatt atttccggaa taaatgaatt ttaccttgtc
     7021 cgtacattcc agatcgttcc tgctgatatc ggtgaggagg tcttcgaggc tatcgaaata
     7081 ttcgggattg ctttggtcct ttttgcggaa ctgaatgatc ttcagcttgt cctctttttt
     7141 ggcgttgtcg agaatacgct gtacatcatc ttcgtcctcg tgtttgatcg atttgctgga
     7201 aaacgtgccg aggaggatcc agacattccg tttgatctgt gtgaagatgg tgtcgatgtg
     7261 catgaaatcg cgtttttttg ggatacgtat gatggttatt ttcttcacta catttcgttc
     7321 gaacaggacg cggatcgcct gatgcgcggc ttcagcggag gtccgttcgc taatgcctat
     7381 aaggaggtgg tctttgcaga tgaccataac gtcgccgcct tcgagggtga ccttccgttc
     7441 gtcgccttcg cgaggcagca gaaaatgatg atgagtatcg ttcagctcga tgatacggtc
     7501 gcggtattcc tcgaaaagcg ggtggttgaa gaagatatat tttgccagta gcgcctcgcg
     7561 cgtccgggcc tttttggccg gcttgttgag taggacatgg tcgttgatga cgatgccgat
     7621 atcgcgggta aagataaaat tggggatagg ggcgaacagg aggcggtcgt catctgtcgc
     7681 tccgctgatg aacgttttgg ccagctgcac cgggtccagt tcctgcagac cctgctggat
     7741 gtcgtacgag caggcttcga tggcgcatac cgacgccacg agcttaagct tgatcgcgtt
     7801 gtccgccagg atatcggcca gcagccattg gagctcgacc actttatcac tgcggaaaaa
     7861 gccctcgtcg ccgggtttat agaaggcgcg gcggttcttt tcatcatcga caacggccag
     7921 ccgacccttt attttaccgg ggtccaggaa atagagcagt attttagtat agtagtcata
     7981 ttccttgcgg cggatggtat ccaggtggac gatatcctcg aagagccagt cctgcgcctt
     8041 cgagggtacc accttaccca gaccgctgtc ggggctatgg accaggacgc ggcgcagacg
     8101 gccgacctcg gaggagacgg aaacggccac ccggttttta tgggggtaat gtttagacat
     8161 agaataaaat tgcgctttcg aatgctgcaa tccggcaaat taggtaattt accagaaaaa
     8221 ttaaggatgg agctgattac cgaccaaacc atcgacctga tcaacgaccg gatccagcga
     8281 atggatgagg gtcagatcgg acggatttgg gattcgttcg ccaaggaaca acccgccctg
     8341 gtcggttatg tgctggggga aagcagggaa ctggaagagc ctgatgcccg cgaggacgtc
     8401 gcatatgtcc tgaccatcat cctgctgagt ttccaggaac tgactccggg gatccctgca
     8461 gttacagagc aggagttcga aaaagccttc aatgaggagt tcgatgagat ggagtctgtg
     8521 ttccaggacg ggttcgacga gtcggatgcg atggcggtga tagaatcttt ttgtcagccc
     8581 gatcttctgc agtttgcggc tgcgactatc tatagtgagg atgcggatga agaggatgca
     8641 agtaatttta cggaagagga ggcggggctg tttattgtta ttttcaagac ggcgattgcg
     8701 ttgctgcata agaaggcggg gagcattata tgatacggcg ctccgccaaa aaaactaccg
     8761 actgttagtt tactaaacaa ctttctctta tttttggggc acgagccggg cgtattccgg
     8821 ccgcaaccaa agcttgtttg cccatgcatt ttcagttgtc agaagagcat ttgatgatcc
     8881 ggaaggccgc ccgggatttt gcccgtaatg aatgtttacc gggggtgatc gaaagggatg
     8941 aacaccagaa atttccaaag gagcaagtga tgaggctggc ggacttagga ttcatgggaa
     9001 tgatggtcga ccccgcctat ggcggatcgg ggatggatac ggtgagctat gtgctggcca
     9061 tggaagagat cagtaagata gatgcgagcg tcagcgtttg catgagtgtc aacaatagtc
     9121 ttgtttgctg gggcctggat acgtacgcca accaggcgca gaaggagcga tatctcaccc
     9181 cgctggcgca ggggaagagg gatggtgaat tgtatatcgg cgcatttttg ctgagcgaac
     9241 cggaggcggg aagcgatgct acgtcgcagc ggacgattgc cgaagaaaag ggcgatcatt
     9301 acctgctgaa cggcgtgaag aactggatca ccaacggtaa ctcggcatcg gtctatctcg
     9361 tcatggcgca gacggacata tcgaagggca gccatggaat caatgcattt atcgtggaaa
     9421 agagctggcc gggcgtggcg gtgggcggca aggagaataa gctgggcatc cgcggcagcg
     9481 atacccatag catatcgttc agcgacgtca aagtgccgaa agagaaccgc ataggagcgg
     9541 atggattcgg cttcaccttt gcgatgaaga cactggcagg ggggcgtatc gggatcgcca
     9601 gccaggcatt gggtatcgcc agcggcgcgt atgagctggc ggtcaattat tcgaaggagc
     9661 gcaaggcatt cggcgcgccg atcgcgcaac atcaggccat ccagttcaag ctcgcggata
     9721 tggctacccg tatcgaggcg gcccggctgc tctgtttacg cgcggcatgg gaaaaggata
     9781 gcaatctcga ctatgcgctt tcttcctcca tggcgaaggt atatgcttcg gagacggcga
     9841 tgtggactgc tacggaggcc gtgcaggtgc atggggggta tgggtatgtc aaggaatatc
     9901 atgtggaaag actgatgcgg gatgcgaaga tcacccagat atatgaggga accagcgagg
     9961 tccagcgcat cgttatcagc cggtcgattt tgaaatagag gctgagcgcg gcggcgcaat
    10021 ggcgggcatt ccggaaatcg gccgtggctc cgcgacggcg gactgcgacc gggacgagcg
    10081 gggtggatca gggatcggca taacggtgca cattatccgt tggtcgagtc atcggcttta
    10141 tggcgatcgc cattgagctc cttcccgaaa ctatatgtaa agctgagctt cagcacccgg
    10201 gtctccggtt tgctgatggc atcctggtag aatacgccgg gcgagtaggt gtccgtacgg
    10261 ctgcgttcgg tattgaacac gtccgacagg ctaagcacga gcgttccggc cttgtgccga
    10321 aggatgtccc ttttgacggc aagatccagg ccacccgagg gaagcgtttt cccctgcgcg
    10381 aaatagctgg ctgcgctata gctgccggtc atctgcagcg taaaggtatt ccacagtttc
    10441 atatccgaat tgagcttggc aaaccagctg aaaccggagt tcgagagacc ctgggacagg
    10501 ttgttggcgt tgatctcggt ctggaagaga ttgaaattgg tcgtggcatt ccaccatttc
    10561 aggatgggga tgcgggcgat gatctcggca ccataggtat tggcatagtt gccgttctcg
    10621 aaggtgctga gcagggtatc gttggaaagc ggtttgttat aagacatcat cgggtcgagc
    10681 gtgttcttca ggtagacagt ggtgctgagc gcaacgccgg atagctgcgt attataggcc
    10741 agctcgagga gattggtatt ttcgggccgg atacctggat tacctctctg cgggttcagc
    10801 ggattgccat aattcgtttg aggagtgatc tgccaccatt gaggccggtt gatacgccgg
    10861 gagaaattca ggtggaggtc gttgtcgtcg tcgaattttt cggtcaggaa aacgctgggg
    10921 taaagccccg attcctgata gccaaaactg gtgttgctat ccagcaggtg accggtatag
    10981 gtgtattttt cataccgcag accggccata tagctgaatt tgcccagctg ttgcgtaaag
    11041 gcgccatagg ccgcataggt attgttgtcg tagctgtaat tgtagctggc attgggatcc
    11101 ctgacataac cctgggccga gctgtcctcg aagtcctgga agctgcggtt gccgcgcagg
    11161 atcgttttga caccgacatc cagcttggac ttcccatcca tcagcggatc cgaataatcg
    11221 ccctgcatgg tgacatgata gccattccca tgaccgttat aacgctgtac attagggctt
    11281 gccagggtag agccgccctt gttcaggaat tgcatgtcat agttgccgta attctttccg
    11341 tgataggtct ctgcggctgc gctcatcgac agtttttcat cttccttttt gaatgtgtgc
    11401 ccgtaatcga aatgggcgtg cgaaaagacg aattggttgc ctgcgtagga gttccgaaag
    11461 ccgttggaat cgagcagcgc gtctttttcc caatatgcag tattctgggt tcccctggta
    11521 gggtggctgc cgaaaccgat gttgcccgaa agactgaagg tattgtgctc atccatgttg
    11581 tagtccgctc cgagccggag gttttggaaa gggccggtgg tcaccgcatg accgtgctgg
    11641 atggccgttg tattggccgc caggttatcg aacgtggtcg tggtattgga gacggagcga
    11701 tgggtatggc ccatatagtt tagccggaaa ttccatttgt gtttgtaaac gttcatgttg
    11761 gcgaaaccgt aggtttcgcc gagcgtgctg ccgacgccgg tgaatgagcc attcataccg
    11821 ggtttgcggt tctttttcag gacgatgttg acgataccgt tgttgccctg cgcatcgtat
    11881 ttggccgaag ggttgggcat cacctcgatg ctttggatct gatcggcggg tatctggtcc
    11941 agggtaagat cggtctgttt gccatcgagg agaatggtgg gagatccgtt gcgcagagtc
    12001 acgttacccg aagcgtcgac attgagcgtg ggtacctggc ggagagcgtc ctgcgccgtc
    12061 ccgcccttgg aggcgaggct ttggtcgaca ttgaagactt ttttatcgat ctctgttttg
    12121 aagataggcc gctgtgcgac caccacggcc gtcgtcatct ccgaagcggt ggacgtcaac
    12181 cggatggtcc cggcatttac gatggctccg ggtgcatgat ggccgccggc tatctggcgg
    12241 tagataggct cgtatccgac gacgctgacc tcgaggatcg agggcgattc gggcagattt
    12301 ttcagggcaa attccccatc cgactggctg atgacgcgag cggcaacgga agagtccttg
    12361 cgtagcagcg tgatcgtagc cgtgggaatg ggttgtttgg tcttgccgtc gataatcttt
    12421 ccggagagga ccgaagagcc cgcatgcggt ttggtctgag cgacggcgaa caatgggagc
    12481 aggaacaaaa gggtatacgt atagtgcttc atcaaggtgc aaaattacgg caaaacgtcg
    12541 cgaaggactt tgttaaattt aaccgctcgt gtacctatct tcgcgttaaa cctattttaa
    12601 catgtcggcc gataaggatg tttatcgaca aattttgtcg gaattgggtg agaatgtgca
    12661 actcgtggcg gtctccaaga ctaaaactgc cgaagatatt ttagcactct atgcagaggg
    12721 gcagcgcgac ttcggtgaga attatgtgca ggagctggcg gataagcaat cgcggttacc
    12781 caccgatata cgctggcatt ttatcgggca tctgcaaacg aacaaggtga agcagatcgt
    12841 tccctttgta catctgatag agggagtgga cagtctgcgg ttgctgaatg agatcgaaaa
    12901 gcaggcggtc cgcgccggcg tggtcatcga cgtgttatta caggtgcata tcgcgcgcga
    12961 ggaaacgaag ttcgggttcg gggcggagga gatcggatcc gcgatgggtg ttcaccggga
    13021 tggtgtgcgg atccgcggac tgatgggtat ggcttcgaat acatccgatc cggatgcggt
    13081 gcggtctgaa ttcagggtgc tgaagggcct gttcgaccgg tggtttccgg atggcggcgt
    13141 cctttccatg ggaatgagcg cggattacaa aacagccatc gaagagggga gtacgatggt
    13201 gcggatcggg agcctgttgt tcgggcggcg gggatgatgt aaattcgcgc ctatggatga
    13261 aatcaagaaa gaagaagaaa agtcgttgaa tttcctggag gagatagtag tagaagacct
    13321 ggcggccggg aaatataaaa gcatattgac ccgattccca ccggaaccga acggatacct
    13381 gcatatcggg catagcaaat cgatcgtttt gaattttggg ctggcgcaaa aatatggcgg
    13441 caagaccaac ctgcgttttg acgataccaa tcccattacg gaagatgtcg aatatgtgga
    13501 cagcatcaaa gaagacatcc gctggctcgg gttcgagtgg gcgggggaat tctatgcttc
    13561 cgactatttc gagcagctgt atcaattcgc cgagaacctg atcctcaagg ggctggccta
    13621 tgtcgatgac agcagcgccg aggagatcgc caaaatgaag ggaacgccga ctgaagcggg
    13681 gacggacagt ccttaccgga gcaggagcgt ggaggagaac ctgcggttgt tcagggaaat
    13741 gcgggcgggg aaattccgcg acggggagaa agtgttgcgg gcgaagatcg atatggcgtc
    13801 acccaatatg catttccgcg acccctttat gtaccgtatc cggcatgctc atcatcatcg
    13861 gaccggcgac gcctggtgca tttacccgat gtatgacttc gcgcatgggc aaagcgattc
    13921 catcgagcat attacccatt cggtctgcac gctggaattc attccgcaca gaccgttgta
    13981 cgactggttc atcgaaaagc tggaaatatt cccttctcat caatatgaat tcgcgcgtct
    14041 caatctcaat tataccgtga tgagcaagcg caaactgctg cagctggtga atgaagggca
    14101 tgtcacagga tgggacgatc cccggatgcc caccatttcg ggtcttcgcc gcaggggcta
    14161 tacgccggaa tcgatccgga tgttcgccga aaaggtaggc gtggccaggc gggaaaacct
    14221 catcgacgtc tcgctgctgg aattctgtgt gcgcgagcat ctgaacaaga tcgcgttcag
    14281 acgcatggta gtcttcgatc cactgaagat cgtgctgacc aactatacgg gcgacgagga
    14341 gctgctatcc agcgaagaca accctgagga cgccagtacg acccaccggg acatcccctt
    14401 cagccgcgag ctgtacatcg agcgggagga ttttatggaa aatccgccga agaagttctt
    14461 ccggttatcg ccgggacaaa aggtgcgact gaaatccgcc tatatcatcc agtgcgatga
    14521 ggtgatcaag gatactgagg gcagggtgat cgggctgcgt tgttcctatc tccccgaaag
    14581 caagagcggg ggggcacagt ccaacatgaa agttcaggct acgatccatt gggtgagcgt
    14641 accgcatgcc attactgcgg aagtgaagtt atatgaccga ttgttccggg tggagaatcc
    14701 ggccggtgag gagggcgatt tcaaggatta tgtcaacccg gattcattgc aggtactgcc
    14761 gggggccttt gcggaacctg ccttaagggg ggctaagttc gatgagcatt atcagttcct
    14821 gcgaaaggga tatttttatt tggacaagga tagctcggag gataagatgg tgttcaaccg
    14881 gacggcgacg ttgcgggata cctgggaaaa agagaaaaaa aagagcggct aaagccgccc
    14941 tttagagtga attcatttat cggttgcctc cgcccccgcg ttgaggacga ttcgcctgca
    15001 tccaatcctg gactttttta gacagatctg ctccgagagc agcgttgaga cgggcaatca
    15061 tagcctcgtt gatcggttgc atctttgcca ttctgtcctc acggctcagt ccctggtttg
    15121 ccgggtccat gaagatctga cgcattgcgg ctctttggtc catctggatg gctttgaccg
    15181 aatcacactg ggtttgcgtc aggttgagat cggtcttcat cttggccaac tgggcctcca
    15241 tacgctggcg cattgcagcc gtatctcctc tcatgccgcc gccctgggcg aaagtagagg
    15301 aaacaaagag gagcgaggtg accagggtcg cgcaggcgat taacaaacgg acgccacgtc
    15361 cgttggccga aggcccatcg ttagattttc tgaaattcat aactcaattg attttaatga
    15421 tggatgtagt accaaatttg tgctaaaata ctatgccacg taatgctttt acgtaggcgg
    15481 caatcctgcg ttttgcttgt cggtcaatct cttctttttt ggcgtgaata ttgctttttg
    15541 ctaatccaac ccatccgcct gccaccgtcg atccaatttc ttttgtcaaa tctaacttcc
    15601 tgattttcaa tttgagagga cctttactta aatctctcta caatgtactt accaatcatc
    15661 accggctatg agctcggcca gttcgtgcgc atactcatgt ggatcttcgt tcctgtggcc
    15721 attctctcca tgttagtgac tacctggctc cattaccgtc ggagaagacg gaattcggag
    15781 gagggcctat tatccatcga cggttttggg ggggaaggct tcgtcatgcc tctccaagtg
    15841 tcc
//
LOCUS       DASYSV010000533         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_365852, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000533 DASYSV010000000
VERSION     DASYSV010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365852"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..2411
                     /locus_tag="VGQ51_16700"
     CDS             285..2411
                     /locus_tag="VGQ51_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEV9038278.1"
                     /translation="MGKKAGSGIEAHWQGEKAGGGIEDVRMGKVELEFSLDEAGVARY
                     AVKYGGKPVVLASRLGFVLKDDSGLSGGFVLRGVDRKEVDETWQPVWGEVKDIRNHYA
                     EMTVHLAGGSGKLIDIQFRVFEDGVGFRYEFPRQAGLKYFIVGDELTQFRMAGDHKTF
                     WIPGDYDTNEYPYTTSALSAVDNRSMVAASTAIAVRVAPDPYAVQTPLMMKTADGLYL
                     NIHEAALVDYPAMQLHVDKGTLTLSASLVPDAVGNKAYLHAPCHTPWRTIIVSDKAAD
                     ILASKMILNLNEPSKLAHTDWIHPMKFVGVWWEMQTGKSTWSYSDFADSLSASGRGNG
                     DSLSAAGSLIPNGRHGANTANVERYIDFAARNHIGGVLVEGWNVGWEDWFGNWKENVF
                     DFVTPYPDFDIRAITAYAASKGVQLIMHNETSGSVTNYERQMDTAFRFMTARGYHAVK
                     TGYVGRIIPRGEHHDGQWMVEHYVRVAAAAARYHVMLDAHEPVRPTGLQRTYPNWLAC
                     EAARGNEYNAFSDGNLPEHETILPFGRLMGGPMDYTPGIFKLRGYSTVAGRQVHTTLA
                     KQLALYVTLYSPLQMAADLPENYEAHMDAFEWIRGVPVDWDDTKILEAEPGDYLTIAR
                     KGKGSENWYLGAIADENARTMKVSLGFLDKGRRYKATVYKDGAGADWKTNPEAYTIEA
                     MEVNSGSELVVRLAPGGGCAVSFVAM"
     gene            complement(2573..4183)
                     /locus_tag="VGQ51_16705"
     CDS             complement(2573..4183)
                     /locus_tag="VGQ51_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6377 domain-containing protein"
                     /protein_id="HEV9038279.1"
                     /translation="MPTRILLFLDLLFLSFSARSQDLSQLNRALEASPIYDSAKTSEI
                     RRERSALESAASKDNLLFTHYQNLYDQYKVFQYDSAFHYARCLQNTAQQLNAPIKIAD
                     ARLKLCFCLLSAGLFKETLDSLRTIDTKGLPDSLQAEYYSLMGRYYYDLGDFDNDDYN
                     TPAYTRIGNAFMDSALNLYPQGSFPAEYYKGLKQLKSGEGKHALTLFKHLLGRPDLPL
                     HQLAVTTSTLSDIYIHNGETDTAIDLLIRAAIADIKSSTKETTAIFNLAELLYKKGDV
                     RNASSYIEVAIRDAVFYGARQRKVQVSAILPLIEGEKIDRVEAQKRLFVTWSVIATGL
                     LLLVILLVITVLRQVKRLTAAQRIITTAHAKEQAVNQRLLETNNLLAEANKIKEEYIG
                     FFFNADSESVARIEKFKKSLEQKIAYRKWDEVIALVNNTDLKKEKEEMLRNFDQVFLK
                     LFPGFIRQFNELFREEDRVQLKDNELLNTDLRIFALIRMGIHENEKIARILEYSVNTI
                     NTYKTKIKNRSIIPNEEFEQRIMAIKTI"
BASE COUNT         1009 a          932 c         1609 g         1093 t
ORIGIN      
        1 tagatgcgga gagagggagc ggttgagata taattgttat agaaaacgaa tataagttgc
       61 tatttgtcat tgcaagtcgt tttttcgtaa ggctaaacta ttcgcgtcac gacaagggga
      121 aaagctgaga ggtttttccc cgctttttta ccattatctt caatatgcag cctcataagg
      181 tcattgcttg cttttgcgta tttttttcat ttgcaatcgt ccatgctcaa cccaccggga
      241 cccgcccgaa gcagggggcg ggcggtggga ttgaggctgt ccggatgggg aagaaagccg
      301 ggagcgggat tgaggcccat tggcagggag agaaagccgg gggtgggatt gaggatgtcc
      361 ggatggggaa ggtggaattg gagttctcgc tggatgaggc gggggtggct cgttatgcgg
      421 tgaagtatgg ggggaaaccg gtggtgctgg catcgcggtt gggatttgtg ctcaaggatg
      481 atagcgggtt aagcggggga tttgtgttgc ggggtgtgga taggaaggag gtcgacgaga
      541 cctggcaacc ggtgtggggg gaagtgaagg atatccgtaa ccattatgcg gaaatgactg
      601 tgcatctggc cgggggatcg ggaaagttga tagacatcca attccgggtg ttcgaggatg
      661 gggtcgggtt ccggtatgaa tttccgaggc aggcgggatt gaagtatttt atcgtcgggg
      721 atgagctgac gcagttcagg atggcgggtg atcataagac cttctggata cccggagact
      781 acgataccaa cgaatatcct tatacgacct cggcgctgag tgcggtggac aaccggtcca
      841 tggtggccgc atcgacggcg atagccgtgc gtgtcgctcc cgacccctat gccgtgcaga
      901 ccccgctgat gatgaaaacg gcggacgggc tttacctaaa catccatgag gcggcgctgg
      961 tggattatcc tgccatgcag ctgcatgtcg acaaaggtac gctgaccttg tctgcgagcc
     1021 tggtgccgga cgcggtagga aataaggcct atctgcatgc gccttgtcac acgccctggc
     1081 ggacgatcat cgtcagtgac aaggcggccg atatactcgc ctcgaaaatg atcctcaacc
     1141 tgaacgagcc gtcgaagctg gctcataccg actggatcca tccgatgaaa ttcgtcggag
     1201 tatggtggga aatgcaaacg ggtaagagca cctggtcgta ttcagacttt gccgacagct
     1261 tgtcggcgtc ggggaggggg aatggggata gcttgtcggc agcgggctcg ctgatcccca
     1321 acggccggca tggggcgaat acggcgaatg tggagcgcta tatcgatttt gcggctcgca
     1381 atcatatcgg cggagtgtta gtggaaggct ggaatgtggg atgggaggat tggttcggca
     1441 actggaagga aaatgttttc gactttgtca cgccttatcc cgactttgat atccgcgcga
     1501 tcacggctta tgcggcgtcg aagggcgttc agctgatcat gcacaatgag acctcgggat
     1561 cggtcacaaa ttatgaacga cagatggata ctgctttccg gtttatgaca gcccggggtt
     1621 atcatgcggt gaagaccgga tatgtgggcc ggatcattcc gcggggcgag catcatgacg
     1681 ggcagtggat ggtggagcat tacgttcggg ttgctgccgc cgcggcgcgg taccatgtca
     1741 tgctggacgc gcacgaaccg gtgcggccta cgggattaca gcggacgtat ccaaactggc
     1801 tggcctgtga ggcggctcgt ggcaacgagt acaatgcttt cagcgatgga aatttgccgg
     1861 agcatgagac gatattgccg tttggcagat tgatgggtgg gccgatggat tatacgccgg
     1921 ggatctttaa acttcgcggg tattcaacgg ttgccgggag acaggtccat acgacgctgg
     1981 ccaagcagct ggctttgtat gttacgttat atagcccgtt gcagatggcg gcggacctgc
     2041 cggagaatta cgaggcgcat atggatgcct ttgagtggat acgcggggtg ccggtggatt
     2101 gggatgatac gaagatactg gaggcggagc cgggggatta tcttacgatt gcgcggaagg
     2161 gaaagggttc ggagaattgg tatctggggg cgattgcgga cgagaatgcg aggacgatga
     2221 aggtgtcgtt aggttttttg gataaaggcc gccggtataa ggcgacggta tataaggatg
     2281 gggcgggggc ggactggaag actaacccgg aggcgtatac gatcgaggct atggaggtga
     2341 atagcggtag tgagctggtg gtgaggttgg cgccgggggg cgggtgcgcg gtgagttttg
     2401 tggcgatgta aggctggaag agtgcgccag ggatcgaagg ggaaagcccg gagcaaagcg
     2461 aggacttgga acgaagagcc cggcgcggcg gtttgccgcg ggcgcccaag taaaagcgaa
     2521 gggcgcgaaa gaggagacgc gtgtcgaaaa gcagtgtcga cgaagttcaa gcctaaatcg
     2581 tctttatggc catgatgcgt tgttcgaact cttcgtttgg gatgatcgat cggttcttga
     2641 tctttgtctt ataggtgttg atggtattga ccgagtactc aaggatgcgg gctatttttt
     2701 cgttttcgtg gatgcccatg cggatgaggg cgaaaatgcg gaggtcggta ttcaggagct
     2761 cattatcttt cagctgtacc cggtcttcct cgcggaacag ctcattgaac tgccggatga
     2821 atccggggaa gagcttgaga aagacctgat cgaagttacg cagcatctct tctttttctt
     2881 ttttcagatc ggtgttgttc accagggcga tgacttcgtc ccatttccgg taagctatct
     2941 tttgttcgag cgattttttg aatttttcga tgcgggcgac cgattcggaa tcggcgttga
     3001 agaagaagcc gatgtattcc tcttttatct tattggcctc ggccagcagg ttgttggtct
     3061 ccagcagccg ttggttgacc gcttgttcct tggcgtgagc ggtggtaatg atgcgttggg
     3121 ccgcggtgag ccgtttgacc tgccggagga cggtgattac gagcaggatg acgagcagca
     3181 gcagaccggt tgcgatgacg gaccaggtga cgaagagacg tttttgggct tcgacccgat
     3241 cgatcttttc gccttcgata aggggcagga tggcgctgac ctggactttt ctctgccgtg
     3301 cgccatagaa gacggcatcg cgtatcgcga cttcgatgta ggaggatgcg tttcgtacgt
     3361 cgcctttttt atagagcagc tcagccagat tgaagatggc ggtcgtctcc ttggtcgagg
     3421 actttatatc ggcgatggcc gcgcggatga gcaggtcgat ggcggtgtct gtctcgccgt
     3481 tgtggatata gatatcgctg agagtcgaag tggtgacggc caattggtgc agcgggaggt
     3541 cgggccgacc gagaaggtgt ttgaagaggg tgagcgcgtg tttgccctcg ccggacttga
     3601 gttgttttag acccttataa tattcagcgg ggaaggaacc ctgcggatag aggtttagtg
     3661 cggagtccat gaaagcgttg cctatgcggg tataggcggg cgtgttataa tcatcgttgt
     3721 cgaaatcgcc gagatcgtaa tagtaccggc ccatcaaaga ataatattcc gcctgcaggc
     3781 tgtcgggtaa tccttttgtg tcgatggtcc tgagcgaatc gagggtctcc ttgaagaggc
     3841 cggcggaaag aaggcagaag catagtttta gccgggcgtc ggcgatcttt atgggggcgt
     3901 tgagctgttg agctgtgttt tggaggcagc gggcgtagtg gaaggccgaa tcgtactgga
     3961 agactttgta ttggtcgtag aggttttggt agtgggtaaa caggaggttg tctttggacg
     4021 cggcggattc cagggcagat cgttcccggc ggatctcgct cgttttggcg gagtcatata
     4081 tcggggaggc ttcgagggca cgattgagct gagacaggtc ctgggaccgg gcggagaagg
     4141 aaaggaataa gagatcgaga aagaggagga tccgcgtcgg cacttttggc taggtagttt
     4201 ttggtcctca aatatacgga atggcggagc cgtaggggta aaaaagaagc ccagataaaa
     4261 atccaggccg ttgctaaaat ccaatatcct attaaaagcc atattactaa ggcaaaatac
     4321 attccactag cagtgtaagc ggcgaaagac gagtttttga ccttaaaatg aggaaaagtg
     4381 gggaatggat tccccaggga aagggtaggg aattcttcaa agtgggggtt tgggtgcggc
     4441 gttgggggtt tgggatgcgg cgtcgggatt ggggtgcggc gttgggggtt tggggtgcgg
     4501 cgggcggccg aaaggaaatg cggcagaaag ggaatggtgg aatgggtggc ggtgttaact
     4561 ttggtaaatg atcaatgaac gggataagga atttttgaga atggcggtgg aactggcccg
     4621 gcggggagta gaggggggca tgg
//
LOCUS       DASYSV010000534         5359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_109216, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000534 DASYSV010000000
VERSION     DASYSV010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109216"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /gene="rho"
                     /locus_tag="VGQ51_16710"
     CDS             <1..820
                     /gene="rho"
                     /locus_tag="VGQ51_16710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEV9038280.1"
                     /translation="KLNLFTTPNDYSTRIIDLFTPIGKGQRGLIVSQPKTGKTMLLKA
                     VANAVAANHPDCYLMIVLVDERPEEVTDMERSVKAEVIASTFDEPAEKHVKVSTIALQ
                     KAKRLVECGHDVIILLDSITRLARAHNTVAPASGKVLSGGVEANAMQKPKQFFGAARK
                     IENGGSLTILATALIDTGSKMDEVIFEEFKGTGNMELQLDRRLSNRRIYPAIDLVSSS
                     TRRDDLLLEREVLQRMNLLRVYLNDMNPEEAMNELLKRMRGTKSNEEFLASMNG"
     gene            complement(895..1125)
                     /locus_tag="VGQ51_16715"
     CDS             complement(895..1125)
                     /locus_tag="VGQ51_16715"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEV9038281.1"
                     /translation="MWEEKNNTLYRKFQFKDFSQAFAFMTRVALAAEKMDHHPNWSNV
                     YNTVEIRLSTHDAGDVVTDKDRKLAKIIDALM"
     gene            complement(1163..1597)
                     /locus_tag="VGQ51_16720"
     CDS             complement(1163..1597)
                     /locus_tag="VGQ51_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HEV9038282.1"
                     /translation="MKLKLNIEDMTGEFFEDTRLLGLVAPVKDYQFCWQLNNRMRMDF
                     RINNDIEIQLTKRQRKYFFGVYEYREDGNSLRHFLYNNQFDGEYLLPEFKHLDFLWLL
                     KGDMVPDEKLALLMGSVRAVPGVQLVMELAQEKIRNKGHLIF"
     gene            complement(1594..2877)
                     /locus_tag="VGQ51_16725"
     CDS             complement(1594..2877)
                     /locus_tag="VGQ51_16725"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV9038283.1"
                     /translation="MIFRIKLLSPILALLLFTVLPSSPVRAQRQKKTDKLLLTNLDAH
                     IRYLSDEKTGVSKMAGEKQAGDYIVSELSKAGLKPKGDNGGWLQGFEIDQGRIVSGDA
                     YFIVNDHPLILNKEYFPLAFSAIGTVSGSPAIALQESGVPWFLDLRDLLENQDGAKTD
                     LLEAIHSKAAAFAKKGATALIVYNTSKTPDNLTFDPKDKREPAAIPIVYVTADAKKKY
                     LRDESASVDIRIRTGFSEKTSSGHNVVGYIDNGASSTVLIGTRYEEDNVAGVAAMIEL
                     ARMLVPSRLKNNNYLFVAFSGIGQGSFGSKFMAGHTPADTHQLNYLLELDRSAPPQDS
                     TYGLIVGGSGSSPAWWGTCREAGKIAFPLHLDSSYARSGDQTYFYSQRVPALVFYMGA
                     DKPGHMLELEVLKYVFDIVKAANTRGKLAFTPTGS"
     gene            3018..>5359
                     /locus_tag="VGQ51_16730"
     CDS             3018..>5359
                     /locus_tag="VGQ51_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase SNF2"
                     /protein_id="HEV9038284.1"
                     /translation="MALPHLIKYVYTNGTDEVIRRGKKIHAIGYVELIEYDELFESVT
                     FRVKDDSYTTYYKVYVQKFKDPKTLSIRCSCPYNLGDICRHEAGALIQLQELLDRNML
                     KAEEVQYDQRHTVIKMKFIDLKTIRLLCSQQTLADAEKYLRTQRARIEYAKDEVVKAS
                     VEIEGQVYQVVLRKNEERNFDTSSDYPDTIHPLSLPKVIVLLQLLHAHGAYYFDSIRN
                     WDKEKNKLLEAYGYSLSDDLQGKFEFSYKEGKPFLRVLDTTIKRVAPVAGIRPRPVEV
                     EAPVTVVEEEKAIGNAQRLGVVFNFNYEGFPGFELDAVAGESNEEQSGYSTKAEKLDL
                     SKFIPVDPFSEFDRQLIPSLRKMQDGEINKYLNRNSPFSGIWENIIHQEEDSLPEETK
                     KLMVEYLHPKLKKLFLELGSNPFVFYLNGHKVFKTINLLPVGLSGDVISPQFSVSAEK
                     DGYEVSCFVKINGQPVDIARNECRSPLLFFYNNNLYLWNKPEDIALIEKFAGGQGRMI
                     PKAEWPAQLRSFVLPLTMDYPVDFGSGLVREIREGDPEKKLLLQEKGDYLVFQPIFSY
                     KGFETRPGGKDEIVVPDGDKVLIVHRNKEAELQFFRKLESLHSAFIHPEGSYSLALKG
                     ADVLKNNWFFLFVDAMEEMKVPVYGFDALKNFRFNTAKPQTKIHISSNTDWFDARVDI
                     VFGDQKVTIADVKKALGNRQQFVPLHDGTLGVLPEEWIKKYSLLFRVGEGKSNQLRLS
                     KYHMSVIDELYEGRNEEELVIRLEEKYEQLREFNRIREVPP"
BASE COUNT         1313 a         1443 c         1386 g         1217 t
ORIGIN      
        1 aaagctgaac ctgttcacaa cgcccaacga ttattccacc cgtatcatcg atctgttcac
       61 gcccatcggc aagggacagc gcggtctcat cgtctcccaa cccaagacgg gtaagacgat
      121 gttgctgaaa gccgtcgcaa acgccgttgc agccaaccac cccgattgct acctgatgat
      181 cgtcctcgtc gacgaacgcc ccgaggaggt caccgatatg gaacgcagcg tcaaggccga
      241 ggtcatcgcc tctactttcg acgaaccggc tgagaaacac gtaaaggtgt ccaccatcgc
      301 gctccaaaaa gccaagcgtc tcgtggaatg cggtcacgat gtgatcatcc tcctcgattc
      361 gatcaccagg ctcgcccgcg cgcacaatac cgtggctccg gcttcgggga aggtgctgtc
      421 gggaggtgtg gaagccaacg ccatgcagaa acccaaacag ttcttcggtg cggcgcgtaa
      481 gatcgagaac ggcggatcat tgaccatcct cgccaccgcc ctcatcgaca caggttccaa
      541 aatggatgag gtgatcttcg aagagttcaa aggtaccggc aatatggaat tacagcttga
      601 ccgccgtctt tccaaccgcc gcatctaccc ggctatcgac ctggtatcct cctcgacccg
      661 ccgcgacgat ctgttgctgg aacgcgaggt gctccagcgc atgaacctgt tgcgcgttta
      721 tctcaacgat atgaacccgg aagaggcgat gaatgaactg ctgaaacgca tgcgtggtac
      781 gaagagcaat gaggaattcc ttgcgagcat gaacggctaa tgtctgccct caatgtccgc
      841 cctccgggcg attcgccgct ggcaaatcct ggctagtcga ctgcggcgcg gaggctacat
      901 cagcgcatct attattttcg ccagcttcct gtccttatcg gtgactacgt cacctgcatc
      961 atgcgtcgac agccggatct ccaccgtatt ataaacattg gaccaattcg gatgatggtc
     1021 cattttttcg gccgcgagcg ctacccgcgt cataaatgca aatgcctgcg aaaaatcctt
     1081 gaattggaat ttccggtaca acgtgttgtt tttttcttcc cacatggcta aagattttta
     1141 acgggcctcc ggcgcccttt tcctaaaaaa ttaaatgtcc tttatttctg atcttttcct
     1201 gtgccagctc catcacaagc tgcacgccgg ggacggcacg gaccgaaccc atgagcaggg
     1261 ccagcttttc gtcggggacc atgtccccct tgagcagcca caggaaatcc agatgcttga
     1321 actcaggcaa taagtattcc ccgtcgaatt gattgttata aaggaaatgc cggagcgaat
     1381 tgccgtcctc ccggtattca taaaccccga agaaatattt tcgctgccgt ttggtcagct
     1441 ggatctcgat gtcattattg atgcggaagt ccatccgcat gcggttgttc agctgccagc
     1501 agaactgata gtccttcacc ggggccacca gacccagcaa acgggtgtcc tcgaagaact
     1561 cccccgtcat atcttctata ttgagcttca gcttcatgag cccgttggag taaatgccag
     1621 ctttccgcgc gtattcgcgg ctttcacaat atcgaataca tattttaaca cttccagctc
     1681 cagcatgtga cccggtttgt cggcacccat atagaacacc agcgccggca cacgctgaga
     1741 atagaaataa gtctgatcac ccgatcttgc atagctgctg tccaggtgta aggggaaagc
     1801 tattttaccg gcctcccggc aggtgcccca ccaggcaggc gaagagccgc tgccgccaac
     1861 gatcagacca taggtgctgt cctggggagg agcggaccgg tccaactcga gcagatagtt
     1921 caattggtgc gtatccgccg gcgtatgccc ggccatgaac ttcgatccga aagacccctg
     1981 gccgatgccg gaaaaggcta cgaacagata gttgttgttt tttagtcgcg aagggaccag
     2041 cattctcgcc agctcgatca tcgcggcgac gccggccaca ttgtcttcct cataccgcgt
     2101 ccctatcagg acggtcgatg atgcgccgtt gtcgatataa ccgactacat tatggcccga
     2161 gctggtcttt tcggaaaaac ccgtcctgat ccggatatcc accgaggcgg attcatcccg
     2221 gagatatttc ttttttgcgt ctgcggtgac atagacgatg ggaatagcgg cgggttcgcg
     2281 cttatccttc gggtcgaagg tcaggttgtc cggcgttttc gatgtattgt agacgataag
     2341 cgcagtggct ccttttttgg cgaaagccgc ggcctttgaa tggatggctt ccaaaagatc
     2401 ggtctttgct ccgtcctgat tttcgagtag atcgcgaaga tcgaggaacc agggcacgcc
     2461 gctttcctgg agggctattg ccggggagcc cgaaaccgtt cctatcgcgc tgaatgccag
     2521 tggaaaatat tccttgttga ggatcagcgg gtggtcgttc acgataaaat aggcatcgcc
     2581 gctgacgata cgaccctgat cgatctcgaa tccctgcagc caacccccat tgtcaccctt
     2641 cggtttaagc ccggccttgc tgagttcgct cacgatataa tcgccggcct gcttttcccc
     2701 cgccatttta ctcacgcctg tcttttcgtc ggacagatag cggatatgcg catcgagatt
     2761 cgtcaataac agcttatcgg tctttttttg tcgttgggcg cgtaccggcg aagagggtaa
     2821 gacggtaaaa agaagcaaag caaggatagg tgacaataat ttaatacgga agatcataaa
     2881 cgcggtttaa cataacgatc tgtggtacaa agagaacccg catcaggggt ctaccaaagg
     2941 taagatggat ttaattttct acactcacct tttttccttt agttttgcag attggccttt
     3001 tcggattaat aaagcatatg gcgttaccgc accttattaa gtatgtatat accaatggaa
     3061 ccgatgaagt gattcgccgg ggaaaaaaga tccatgccat tggatatgtt gaactgatcg
     3121 aatacgacga gctgttcgag tccgttactt tccgggtgaa agatgacagc tatactacct
     3181 attacaaggt ctatgttcag aagttcaagg acccgaagac gctttccatc agatgcagtt
     3241 gcccctataa tctgggcgat atctgtcgtc atgaggcggg agcgctgatc cagctgcagg
     3301 aactgctcga ccggaatatg ctgaaagccg aagaagtcca atacgaccag cgccatacgg
     3361 tcatcaagat gaaattcatc gatctgaaga ccatccgtct cctttgctcg caacagacgc
     3421 tcgccgacgc cgaaaaatat ctgcgcaccc agcgtgcgcg tatcgaatac gccaaagacg
     3481 aggtggtgaa ggcatccgtc gagatcgaag gacaggtgta ccaggtggtg ctccggaaaa
     3541 acgaggaacg gaatttcgat acgagcagcg attatcccga taccatccac cccctctcgc
     3601 tacccaaagt gatcgtcctg ctgcaattac tccatgcgca cggcgcctac tatttcgaca
     3661 gcatccgcaa ctgggacaag gaaaagaaca agttactgga agcatacggt tattcgctct
     3721 ccgatgacct gcagggtaaa ttcgagttct cctataagga aggaaaacct ttcctgcgtg
     3781 tgctggatac gacgatcaag cgtgtagctc cggtagcggg catccgtccc cgcccggtgg
     3841 aggtagaggc cccggtcacg gtggtcgaag aggaaaaagc gatcgggaat gcgcagcgtc
     3901 ttggcgtggt cttcaatttc aactatgagg gtttccccgg atttgagctg gatgcggtag
     3961 cgggtgaaag caatgaagaa cagagcggct attcgacaaa ggccgaaaag ctcgatctct
     4021 ccaaattcat ccccgtcgat cccttttccg aattcgaccg gcagctgatc ccttcgcttc
     4081 gcaagatgca ggacggcgag atcaataaat acctcaaccg gaattccccc ttcagcggca
     4141 tctgggagaa tatcatccat caggaagagg actccctccc cgaggaaacg aaaaaactga
     4201 tggtggaata tctccacccc aaactgaaaa agctgttcct cgagctcggc tccaatccat
     4261 ttgtattcta tctcaacggt cataaggtct ttaagacgat caatctgctg cccgttggtc
     4321 tttcaggcga cgtgatttcg ccgcaattct cggtctcggc ggaaaaggat gggtacgagg
     4381 tgagctgttt cgtcaagatc aatggccagc cggtagacat tgcacgcaac gagtgcagga
     4441 gcccgctgct ttttttctat aacaataacc tttatctctg gaacaagccg gaggatatcg
     4501 cgctgatcga aaaattcgcc ggcggacaag gtcggatgat ccccaaagcc gagtggccgg
     4561 cacaattgcg ctcgttcgtg ctgccgctga cgatggacta tcccgtggat ttcggcagcg
     4621 ggctggtacg ggagatacgg gaaggagacc ctgaaaaaaa attattgctc caggaaaaag
     4681 gagactacct ggtcttccag ccgatattct cctacaaggg ttttgagacc cggcccggcg
     4741 gaaaggacga gatcgtggta ccggacggcg acaaagtcct gatcgtccat cgcaataaag
     4801 aagccgagct gcaattcttc cggaagctgg agtctttgca ttctgccttt atccatccgg
     4861 aaggttcgta cagcctggca ttaaaaggag cggacgtatt gaagaacaac tggttcttcc
     4921 tcttcgtgga cgcgatggaa gagatgaaag tgcccgtcta cggcttcgac gcgctgaaga
     4981 acttccggtt caataccgcc aaaccgcaga caaagatcca tatcagcagc aataccgatt
     5041 ggttcgacgc gcgggtggac atcgtattcg gcgatcagaa ggtgactatc gcggatgtta
     5101 aaaaagcgct cggcaaccgc cagcagttcg tcccgctgca tgatggtacg ctcggggtgc
     5161 tgccggaaga atggatcaag aaatattcgc ttcttttccg tgtcggagaa ggcaaaagca
     5221 atcagcttcg cctgtcgaaa tatcatatga gcgtgatcga cgagctctat gaaggccgca
     5281 acgaagaaga gctggttatc cgcctggaag aaaaatatga gcagctgcgg gagttcaacc
     5341 gcatccgcga agtgccccc
//
LOCUS       DASYSV010000535        11058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2416471, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000535 DASYSV010000000
VERSION     DASYSV010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416471"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..611)
                     /locus_tag="VGQ51_16735"
     CDS             complement(30..611)
                     /locus_tag="VGQ51_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9038285.1"
                     /translation="MTLLRKKTDHELIHQFRDGDLHALEALIVRHKDKLYTSILFLVK
                     DKYLAEDIFQDVLIKIIDTIRGGRYTEEGKFLPWAMRIAHNLCVDHFRKVKRTPAIKT
                     SDDRDIFEVIHFTEEGADTKMMKRQSHDKVRQMLDLLPEDQREVIILRHYADLSFKEI
                     AALTNCSINTALGRMRYGLINLRKMMTEKKIAL"
     gene            978..3785
                     /gene="uvrA"
                     /locus_tag="VGQ51_16740"
     CDS             978..3785
                     /gene="uvrA"
                     /locus_tag="VGQ51_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV9038286.1"
                     /translation="MASSSKVKPKAKEKLIRAESTDAIFIKGARVHNLKNIDVSIPRN
                     KLVVVTGVSGSGKSSLTIDTLFAEGQRRYAESLSAYARQFMARMDKPDVDYIKGLCPA
                     IAIEQKVITRTPRSTVGSMTEIYDYLRLLFARVGKTYSPVSGRIVKKDDVTDVVSAIS
                     ALAPGDKVLLLTAFKQHRNRDTKEELNILLQKGFSRLYAGGETLRIEDLMEKPSPDLP
                     PSTWILVDRLVAKTFDEDDLHRIADSVGTAFYEGEGAMQLEINGTKTLPFSNRFELDG
                     ITFEEPVPNLFSFNNPFGACPTCEGFSQVLGIDPDLVIPDKRLSLYEGAIAPWKGEKL
                     VWWKDQFIKAAKKFDFPIHKPVIDLSKTQYKTLWEGNEHAQGINEFFAEVERNLYKVQ
                     YRVLLSRYRGRTDCPDCKGYRLRKEALYVKIGGRHIGELCEVPVVQLTQWFEELQLEP
                     HEQAIAKRILIEIRHRLSTLMDVGLGYLTLNRLANSLSGGESQRIQLTRSLGSNLTNS
                     LYILDEPSIGLHSRDTQRLIRVLKELRDLGNTVVVVEHDEMMMREADYIIDMGPLASH
                     LGGEVVAAGDYETLIHDGNSLTGKYLKGELAITPPATPRKWKQSLIIEGARQNNLKDL
                     TVEIPLGVLCVVSGVSGSGKTTLVKQILYPALQKLKGEPGEKVGLHKTIKGNVDDISQ
                     VEMVDQNPIGKSSRSNPVTYIKAYDEIRDLFARQPLSKMRGFQPKHFSFNVDGGRCDT
                     CKGEGEQVVEMQFLADVHLTCEVCHGRRFKEEILEVNYREKNIFQILEMSVDEALEFF
                     KDEKDIIQKIKPLSDVGLGYVKLGQSSDTLSGGEAQRVKLASFLGKGRAQGHILFIFD
                     EPTTGLHFHDIRKLLTSFDALIGQGHSILVIEHNTDVIKSADWVIDLGPEAGDGGGSL
                     VYAGTPAGLKKVKDSYTGRYF"
     gene            complement(3803..8350)
                     /locus_tag="VGQ51_16745"
     CDS             complement(3803..8350)
                     /locus_tag="VGQ51_16745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV9038287.1"
                     /translation="MLKKFIERPVLSTVVSVILLLLGVLSVMSLPVTLFPDIAPPSVV
                     VSANYPGANAEVVARSVATPLEESINGVENMTYMTSNSSNDGSMTLTVYFKQGTNPDI
                     ASVNVQNRVAKATSLIPQEVIQAGISTQKQQNSIIMFIAVYSEDSTYDETFLQNYIKI
                     NLIPRLQRIPGVGDAQVFGAKDYSMRIWLKPDRLAAYDLSPMDVTSAIKDQSLEAAPG
                     RLGQNSKEVFEYVLKYKGKLNNTQDYENIVIKSNSDGSVVRLKDVARVEFGSYSYGSN
                     SRLNGHPTSGCAVFQVAGSNANDILTQAKELIDKFETQLPKGIHTSIMYNSKEFLDAS
                     ISQVKETLIEAFILVFIVVFIFLQDLRSTLIPAIAVPVAIVGTFFFLQLFGYTINLLT
                     LFALVLAIGIVVDDAIVVVEAVHSKMERTGLASRPATVAAMSEISGAIVSITLVMAAV
                     FIPVGFLKGPAGVFYRQFAFTLAIAILISAVNALTLSPALSALFLRNNHAEGGGGKRR
                     LGARFFVAFNAGFKAMTDKYIGSLRFLFRRKWITIGVLAALAVLSYMMMESTPSGFIP
                     TEDQGFLLYAVNTPPGSSLEQTHHAVEEIDSIIKNDPLTDRRYNIEGFNFISGANASP
                     YGAGFVRMKPVDQRGPLKDMDAITASMTQKVAMQVKDAHAFFFTFPTIQGFGNVAGFE
                     FMLEDQTNGSLDKLGATAGHLIGALMQRKEIAYAFTTFASGNPQYQMVVDNDKAKQLG
                     VSIADLMETMQIYYGSSFVDDFNRFGKYYRVMAQADVPYRTNEASLNGIYVKNGQGQL
                     VPVKELISLKRVYGPETVTRNNLYNAVTINGVPKPGYSTGDAIKAIEETTAQVLPRGY
                     GIEWTGMTREEKSSGSQMIFTFILSLVFVYFLLAAQFESYILPLAVVLSIPVGVFGVF
                     AFLKMFGIDNNIYVQVSLIMLVGLLAKNAILIVEYAVQRRRAGQDLWASALEAAQLRL
                     RPILMTSFAFIAGLLPLMRATGASALGNRSIGTGAVGGMLTGVILGVFVVPVLFIIFQ
                     YLQERLVFKPVRVQPAAATLGVLALVVTLGLGGCKVSKDVATPSPELPQTYANAIPST
                     DTTSVADLPYKSFFADTDLQQLIDSAITRNYDMQVALQNIRSSRLVLRQSKLGYLPDL
                     TLQVTGAYNRPSDNSLNGISTKQFLGTDHIDDYNANVGLSWEADIWGKIRNQQSRALA
                     AYLQTQEARKALQTSIVADVANGYYNLLMLDAQLGIARENLALNDSTLRIIRLQFDAG
                     DVTALGVQQAAAQELAAAGLIPQLQQSILLQEDALSVLTGRLPGGIARGRKLEDIPVR
                     DDLSAGVPAGMVSRRPDVRSSELDLTIANANVGITKAQLYPSLMITAQGGLNTFTASN
                     WFNIPGSLFGMVAGGITQPLFQRRQLKTQYELAKVDREKTVIRFRQSVLIAVGEVSDA
                     LARMDKLKEQRVVAASRVDTLRQAIVNAGSLFRNGMANYLEVITAQSNVLQSELELAS
                     IKKGQLSAEVELYRSLGGGWK"
     gene            complement(8372..9529)
                     /locus_tag="VGQ51_16750"
     CDS             complement(8372..9529)
                     /locus_tag="VGQ51_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9038288.1"
                     /translation="MRKLLVLSIILAAATIYSFSSCNQAKGTQAAPPPQSLPVFPIAA
                     SAATTYLEYTATLEGKTNVEIRPQVSGYLDKIYVEEGSFVRAGQPLFKINDRPYDAQL
                     RNTEADVEAARANMEKAAIEVHRLQPLVANHVISDVQLRSAQAAYDQAKAQVSQAEAA
                     GKNAGINLGYTLLKAPVDGFIGRIPFKVGALVGKGETQALTVLSDVKEVYAYFSMSEN
                     DFLVWSEQATGKTVQEKIQSMPPVELQLADNSIYGPKGHVQLMEGQFDKTMGTVSFRA
                     IFPNTDGLLRTGSTGKVRIPQASSGVMPVPQQATYELQDKVFVFVVGDSNKVSSQPLH
                     IVGKTTGWWLVDKGVKPGDRIVFAGMDRLQDGAVINPQPLKADSIYHVMPL"
     gene            complement(9641..10243)
                     /locus_tag="VGQ51_16755"
     CDS             complement(9641..10243)
                     /locus_tag="VGQ51_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV9038289.1"
                     /translation="MGSKERIHRLKEQTRVNILDAAYDIVKDEGWQALSMRKIADKIE
                     YTAPIIYEYYANKEAILYELTKKGFMMLAERMQAACDRNEDPAAQLEALWLAYWNFAF
                     EEKEYYQLMFGVEVNCCLMDEMFPTGTTPYDIAARPIAEMMGIGDLDDDRVCTKYYTY
                     WSIIHGLISINIVRKGTSDEINRQILRDAITGITRSLKKQ"
     gene            complement(10434..>11058)
                     /locus_tag="VGQ51_16760"
     CDS             complement(10434..>11058)
                     /locus_tag="VGQ51_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV9038290.1"
                     /translation="IGVVKDFHYQGLQEEIRPLSMRIEPDGCNMLSVKVSTSNLKNTI
                     AAIEKTWHTVIPSRPFSYFFVDDMFDRQYRAEDRFGKLFLYFSVLAILISCLGLLGLA
                     SYSTMQRTKEIGVRKVLGASVTGIVRLLSRDFLVLIGIAFVVATPLSFWLMKRWVDGF
                     ASRINIYSAWWIFLAAGLTAVLIAMLTISFQAIKAAYANPVDSLRTE"
BASE COUNT         2471 a         3048 c         2954 g         2585 t
ORIGIN      
        1 catcttttca atcctttgag taaaattttt tacaaagcaa tctttttttc agtcatcatc
       61 ttgcgcagat tgatcaggcc ataacgcatg cgaccgaggg cagtgttgat gctgcaattg
      121 gtcaatgccg caatctcctt gaaggaaaga tcggcataat gcctcaggat gatcacctca
      181 cgctggtctt caggcaacag atcgagcatc tgccttacct tgtcatgact ctggcgtttc
      241 atcatctttg tgtcggcgcc ttcttccgtg aaatggatca cttcgaagat gtcccggtca
      301 tcgctcgtct tgatggcagg ggtcctcttg accttccgga aatgatcgac acaaagattg
      361 tgcgcgatcc gcatggccca gggaaggaat ttgccttctt ccgtatagcg gccgccacgg
      421 atggtgtcga tgatcttgat caaaacgtcc tggaaaatgt cctcggcgag atacttgtcc
      481 tttacgagga agagaatgga ggtgtacagc ttatccttat gacgaacgat caatgcctct
      541 aaagcatgta agtcgccgtc cctaaattga tggataagtt cgtggtcggt ttttttgcgt
      601 agcaacgtca taaacttcta cggttttaaa aggtgatagg atatattttg gattttaaga
      661 tggcttggtt tttctgtgta gaagctaact ctataggcaa cgctttttta acagttattc
      721 aatgatattc acaatccgac tacctctgtg tacaactatt ccttgtatga atagagttta
      781 aagatataca atttttggaa taaacagcat tttgggaatt tttttgtttt caaattttac
      841 aaattcccca aaaccggtaa gggtaacact taatttgtct tatcgcggcc tgaaatcttc
      901 cgaaaagagt agatattttt ttcctaattt ttttgggaaa ctttcgtctt ttttggtgta
      961 ggtttgttat attatatatg gcaagttcga gtaaggtaaa accgaaggca aaggagaaat
     1021 tgatcagggc agaatctacc gacgccatct ttattaaagg tgcgcgcgtc cacaatctta
     1081 aaaatatcga tgtttcgatc ccccgcaaca agcttgtggt cgttaccggc gtgtccggct
     1141 cgggaaaatc ttccctgacc atcgacacac tgttcgccga aggccaacgg cggtatgcgg
     1201 aaagcctcag cgcctacgca cgtcagttca tggcccgtat ggacaaaccc gacgtggatt
     1261 atatcaaagg gctctgcccg gccatcgcca tcgaacaaaa ggtcattacc cgcactccac
     1321 gttccacggt gggttccatg acagagatct atgattatct gcgtctcctg ttcgccaggg
     1381 tgggtaagac ctattcaccc gtcagcggca ggatcgtaaa aaaggatgac gtcaccgacg
     1441 tggtatccgc catctcggca ctggcacccg gcgataaggt actcctcctc accgccttca
     1501 aacaacatcg gaaccgggac acgaaggaag agctcaatat cctgctccaa aaaggattct
     1561 cccggctcta cgccggcggt gagacactgc ggatagagga tctgatggaa aaaccttccc
     1621 ccgacctccc gccgtccacc tggatcctcg tagaccgcct cgtcgcaaaa acattcgatg
     1681 aagacgatct tcaccgtatc gccgattcgg tcggcacggc attttatgaa ggcgaaggcg
     1741 ccatgcagct ggagatcaat ggtacgaaga cccttccgtt ctccaaccgg tttgagctgg
     1801 acggcattac gttcgaggag cctgtcccca acctgttctc tttcaataat cccttcggcg
     1861 cctgccctac ctgcgaaggc tttagccagg tgttgggcat cgatcccgat ctggtcatcc
     1921 cggacaaacg cctctcgctg tatgaaggcg ccatagcgcc atggaaagga gaaaaattgg
     1981 tctggtggaa ggaccagttc atcaaggcag ccaaaaaatt cgacttcccg atccataaac
     2041 cggtgatcga tctctccaaa acacaatata aaacactctg ggaaggcaac gagcacgcgc
     2101 aaggcatcaa cgagttcttc gcggaagtgg aaaggaatct gtataaagta cagtaccgcg
     2161 ttttactgtc ccggtatcgc ggccggaccg attgccccga ttgcaagggc tacagactgc
     2221 ggaaagaggc tttgtacgtg aagatcggcg gcagacatat tggagagctc tgcgaggttc
     2281 cggtcgtcca gctgacccaa tggttcgaag agctgcaatt ggaaccgcac gagcaggcta
     2341 tcgccaaacg tatcctcatc gagatccgtc atcgtttgtc aaccctcatg gacgttggac
     2401 tgggttatct gacgctgaat cggctggcaa atagtctcag tggcggcgaa agccagcgca
     2461 tccagctgac ccgaagtctc ggcagcaatc tcaccaattc attgtatatc ctcgacgaac
     2521 cgagtatcgg tctccactcg cgggatacgc agcggctgat ccgcgtactc aaggagcttc
     2581 gcgatctggg gaataccgta gtcgtagtcg aacacgatga aatgatgatg cgcgaggcgg
     2641 attatatcat cgatatggga ccattggcca gccatctggg gggcgaggtc gttgcggccg
     2701 gcgattatga gaccctgatc catgacggga acagccttac cgggaaatac ctgaaaggcg
     2761 agctggctat caccccgccg gccaccccgc ggaaatggaa acagtccctg atcatcgaag
     2821 gcgcacggca aaacaacctg aaggacctga cggtggagat accgctcggc gtcctgtgcg
     2881 tcgtcagcgg cgtcagtggc agtggaaaaa cgacactggt aaaacagata ctgtatccgg
     2941 cattgcagaa gctgaagggc gagccgggcg aaaaggtcgg tctgcataag actatcaagg
     3001 gtaatgtcga cgatatctcg caggtggaaa tggtcgatca gaatcctatc ggcaaatcct
     3061 cccgcagcaa tcccgtcacc tatatcaagg cctatgacga gatacgcgat ctattcgcgc
     3121 gtcagccgct gagcaagatg cggggtttcc agcccaaaca tttttccttc aacgtcgacg
     3181 gcggccgctg cgatacctgc aaaggcgagg gcgaacaagt cgtggaaatg cagttcctcg
     3241 cggatgtcca cctaacctgc gaggtctgtc atggacgccg gttcaaggag gagatactcg
     3301 aggtcaacta ccgggaaaag aatattttcc agatattaga gatgagtgtg gatgaggcgc
     3361 tggaattctt taaagatgaa aaggatatca tccagaagat aaaaccgctt agtgacgtgg
     3421 gtctcggcta cgtaaaactg ggtcagtctt ccgacaccct ttccggtggc gaagcacagc
     3481 gtgtaaaact tgcctccttc ctcggcaagg gccgcgcaca agggcatatc ctttttatct
     3541 tcgacgaacc gacgacgggt cttcacttcc atgatataag gaaactgctg acgtctttcg
     3601 acgcgctgat cgggcaggga catagcattt tagtcatcga acacaatacg gatgtgatca
     3661 aatccgccga ctgggtcatc gatctgggtc ccgaggcggg ggatggaggc ggcagcctgg
     3721 tctacgctgg aacgccggcg ggactgaaaa aggtaaaaga cagttacacc ggccgctatt
     3781 tctagcgacc ggtggctgcc catcatttcc atcctcctcc gaggcttctg tacaattcca
     3841 cttctgcgct caattgtcct ttctttatcg atgcgagttc caattcgctt tgtaatacgt
     3901 tgctctgcgc cgtgatcacc tcgagatagt tcgccatgcc gttgcggaac agactgccgg
     3961 cattgacgat cgcttgccgc agggtatcca cccgcgatgc cgccaccaca cgctgctctt
     4021 tcagcttgtc catccgggcc agcgcatcgg agacctcgcc tacggcgata agcaccgact
     4081 gccggaaccg gatcacggtc ttttccctat ccacctttgc cagctcatac tgcgtcttca
     4141 actgtcgccg ctgaaacagt ggctgcgtga tgccgcccgc taccataccg aacaacgatc
     4201 cggggatgtt gaaccaattg ctggcggtaa aggtattcag accaccctgc gccgtgatca
     4261 tcagcgaagg atacagctgt gccttggtga tcccgacatt ggcattggca atggtcaggt
     4321 ccagctcgga gctgcgcaca tcgggtcgcc ggcttaccat tccggcaggg acgcctgccg
     4381 acaggtcatc tctcaccgga atatcctcca gtttccgtcc ccgcgctatt ccgccgggaa
     4441 gcctgcccgt caggacgctc aatgcgtctt cctgcaagag gatgctctgt tgcaattgcg
     4501 ggatgagtcc ggcggcagcc agttcctggg ctgccgcttg ttgtacgccc agggccgtca
     4561 cgtctcctgc gtcgaattgc agccggatga tccttagcgt ggagtcgttc agtgcaagat
     4621 tttcacgcgc gatacccagc tgtgcatcca gcatgagaag attgtaatag ccgttggcta
     4681 cgtcggcgac gatactcgtc tgcaaggcct tccgcgcctc ctgtgtctgc agataggctg
     4741 ccagagcacg tgattgctga ttccggatct tgccccagat gtccgcttcc caggagagcc
     4801 cgacgttggc attgtaatcg tcgatatggt ccgtcccaag gaattgcttg gtggatatcc
     4861 cattcaggct gttgtccgaa gggcggttat aggcccccgt tacctgcagc gtcaagtccg
     4921 ggagatatcc cagcttcgat tgtctgagca cgagccgcga agaccggata ttctgcaacg
     4981 ccacctgcat atcgtagttc cgggttatcg cgctgtcgat cagctgttgc agatcggtgt
     5041 ctgcgaaaaa acttttataa ggcaggtcgg ccacgctggt cgtatcggtg gagggtatcg
     5101 cgttggcgta cgtctgcggc aattcaggcg acggcgtcgc cacgtctttc gagaccttac
     5161 atccacccaa acccagcgtc acgacgaggg ccagaacccc cagcgtcgcc gcagcgggct
     5221 gcaccctgac cggtttgaag accaaccgct cctgcaaata ctggaagatg atgaacagca
     5281 ccggcaccac gaataccccc agtatcacac ccgtcagcat tccgccgacc gcgcccgtcc
     5341 cgatcgatcg gttcccaagc gccgaggctc ccgtggcccg catcaatggt agcaaccccg
     5401 cgatgaaggc aaacgaggtc attaggatcg gacgaagccg caactgcgcc gcctcaagcg
     5461 cggaagccca taggtcctgt cccgccctgc ggcgctggac cgcatattcc acgatcagga
     5521 tcgcattctt cgccagcaga ccgacgagca tgatcaggct cacctgaaca tagatattat
     5581 tgtcgatgcc gaacatcttc agaaaggcaa atactccaaa aacaccgacc ggtatcgaca
     5641 gcaccacggc cagcggcagg atatagcttt cgaattgggc tgccagcagg aaataaacaa
     5701 agaccaggct caggatgaac gtgaagatca tctgcgaacc ggaactcttt tcctcgcgcg
     5761 tcattcccgt ccattcgatg ccgtatccgc ggggcaatac ctgtgcggtc gtttcctcga
     5821 tggccttaat ggcgtcaccg gtactataac ccggcttggg cacgccgttg atggttacgg
     5881 cattgtaaag gttattgcgc gtgacggtct cggggccgta aacgcgtttc agcgagatca
     5941 gctctttcac gggcaccagc tgaccctgcc cgttctttac atagatgccg ttgagcgagg
     6001 cttcattggt gcggtaaggg acgtcggcct gcgccatcac ccgatagtat ttgccaaaac
     6061 ggttgaagtc gtcgacgaag ctgctgccgt aatagatctg catcgtctcc ataagatcgg
     6121 cgatcgagac gcccaattgt ttggctttgt cattgtccac caccatctgg tattgcggat
     6181 tccccgatgc aaacgtcgtg aaggcatatg cgatctcctt cctctgcatc agcgccccga
     6241 tcaggtgccc ggccgtcgca cccagcttgt ccagcgaccc gttggtctgg tcttccagca
     6301 tgaattcgaa acccgccaca ttcccgaaac cctgtatcgt cgggaaggtg aaaaagaagg
     6361 cgtgggcgtc cttcacctgc atagcgacct tttgcgtcat cgaggcggtg atcgcatcca
     6421 tatctttgag aggtccgcgc tgatccaccg gtttcatccg gacaaaaccc gctccatagg
     6481 gggaagcatt ggctccggag ataaagttga atccttcgat gttgtagcgg cggtcggtga
     6541 gagggtcgtt cttgataatg ctgtcgatct cctctacggc atgatgcgtc tgttcgagag
     6601 agctccccgg cggggtattg acggcataca gcaggaaacc ctggtcttcc gtcgggatga
     6661 aaccgcttgg cgtgctttcc atcatcatat aagagaggac cgccagcgca gccagcaccc
     6721 cgatggtgat ccatttgcgg cggaaaagaa aacggagact tccgatgtat ttatccgtca
     6781 tcgccttgaa accggcgttg aaggccacga agaaacgcgc ccccaggcgt cttttaccgc
     6841 cacccccctc agcatgattg ttgcgcagga agagcgcgga cagtgccggg gacagcgtca
     6901 gcgcattcac cgccgagatc aggatggcga tcgccagcgt aaatgcaaac tgcctgtaga
     6961 atacacccgc cggacccttt aaaaagccca ccggaatgaa tacggccgcc atgacgaggg
     7021 tgatcgagac gatagccccg gagatctcgc tcatcgcggc cacggtcgcc ggtctcgatg
     7081 ccagtcccgt tcgttccatc tttgaatgca ccgcctccac gaccacgatc gcgtcgtcca
     7141 ccacgatgcc gatggccaga accagcgcaa acagcgtcag caggttgatg gtatacccga
     7201 acagctgcag gaaaaagaag gtgcctacga tggctacggg tacggcgatg gccggtatca
     7261 gcgtcgaacg gaggtcctgg aggaaaatga acaccacgat gaacaccagg ataaaggctt
     7321 cgatcagcgt ctccttcacc tgggagatcg atgcatcgag gaactccttg gagttgtaca
     7381 tgatcgaggt atggataccc ttcggcaatt gcgtttcgaa tttgtcgatt agctccttgg
     7441 cctgtgtcag gatatcattg gcattgcttc cggccacctg gaaaacggca cagccggagg
     7501 tcggatgccc gttcagcctg ctgttcgaac catagctata gcttccgaac tctacgcgcg
     7561 ccacgtcttt aagacgtact acactgccgt cgctgttgga tttgatgact atgttctcat
     7621 agtcttgtgt attgttgagc ttgcctttgt atttcagaac gtactcgaat acctctttgc
     7681 tgttttgacc gagccgcccc ggggccgcct ccaaactctg atctttgata gccgaggtta
     7741 cgtccatcgg cgagagatcg taggccgcga gtctgtcggg tttgagccag atgcgcatcg
     7801 aataatcctt agcgccgaag acctgcgcgt cacccacccc ggggatacgc tgtagtctgg
     7861 ggataagatt gatcttaatg tagttctgta agaatgtttc gtcatacgta ctgtcctcgg
     7921 aatacacggc tatgaacatg atgatactgt tctgctgttt ttgcgtcgag atgcccgcct
     7981 ggatcacctc ctgggggatg aggctggtcg ccttggctac gcggttctgc acgttgacgg
     8041 acgcgatatc ggggttagtg ccctgtttga aatagacggt cagcgtcata ctgccgtcgt
     8101 tgctggagtt ggaggtcatg taggtcatgt tctccacccc gttgatggat tcttccagtg
     8161 gggtagcgac cgaccgggcc actacttcgg cattggcgcc gggatagttg gcggacacga
     8221 cgacgctggg aggtgcgata tcggggaata gcgtgacggg cagtgacata acggacagca
     8281 cgccgagtaa gagcagtata acggacacga cagtggataa caccggtcgc tcgatgaatt
     8341 tcttaagcat aaatgagttt gttgttgatt cttatagcgg catcacatga taaatgctgt
     8401 cggctttcaa tggttgggga ttgatgacgg cgccgtcctg gagacgatcc atccctgcaa
     8461 atacaattct atcaccgggc tttacacctt tatcgaccag ccaccagccc gtggtcttac
     8521 cgacgatgtg cagcggttgg ctggacactt tattgctatc gccgacgacg aaaacgaata
     8581 ctttgtcctg tagctcgtag gtcgcctgct gcgggacggg catcacaccg gaactggcct
     8641 gcgggatacg caccttgccg gtgcttccgg ttcgcaacag tccgtcggtg tttgggaaaa
     8701 tggcccggaa gctgacggta cccatcgtct tgtcgaactg gccttccatc agctgtacgt
     8761 ggcctttcgg cccatagatg ctattgtccg cgagctggag ctcgacgggc ggcatgcttt
     8821 ggatcttctc ctgcaccgtc ttgcccgtgg cctgttccga ccagacgagg aaatcgttct
     8881 cgctcatcga gaaataggca tatacctcct tcacgtccga cagtaccgtc agtgcctgcg
     8941 tctcgccttt gccgaccagt gcgccgacct tgaagggaat gcgcccgatg aatccgtcga
     9001 ccggcgcttt cagcagcgta tagccgaggt tgatacccgc atttttaccg gctgcttccg
     9061 cctggctgac ctgtgccttg gcctgatcgt aggcggcctg cgcagaccgg agctgaacgt
     9121 cggagatgac atggttggct accagtggtt ggagacgatg gacttcgatg gcggcctttt
     9181 ccatattggc tctggcggcc tcgacatcgg cctcggtatt tcgcagctgg gcgtcgtagg
     9241 ggcggtcgtt tattttgaac aggggctgac ctgcacgtac gaaagaccct tcttcgacat
     9301 agatcttatc gaggtagccg ctgacctgcg gccggatctc gacgttggtc ttgccttcca
     9361 gcgtggccgt atattcgaga taggtcgtgg ctgcagaagc cgcgatcggg aagacgggta
     9421 aggactgcgg tggcggagcg gcttgtgtgc ctttcgcctg attgcagctg ctaaatgaat
     9481 agatggttgc agctgcgagg ataattgaca aaactaataa ctttctcata acgttattaa
     9541 actgtttaat ggtgttaaat ccatggcgtg agctatcctt atgacgcgcg aagcgcgtca
     9601 aactggacaa tcgttagaaa ctttttcttg tgggttggat ctactgtttc ttgaggcttc
     9661 gggtgatccc ggtgatcgca tccctgagga tctgccggtt gatctcgtcg gaagtcccct
     9721 tccggacgat gttgattgag atcagtccgt gaatgatcga ccagtaggta tagtatttcg
     9781 tacaaacccg gtcgtcatcc agatcgccga tccccatcat ttccgcgata gggcgcgcgg
     9841 ctatatcata gggggtggtg cccgtgggga acatttcgtc catcagacag cagttgactt
     9901 cgacgccgaa catcagctgg taatattcct tttcctcgaa ggcgaagttc cagtatgcca
     9961 gccaaagcgc ttcgagctga gcggccgggt cttcgttccg gtcgcacgct gcctgcatcc
    10021 gctccgccag catcatgaag cctttcttcg tcagctcata cagtatcgct tctttgttgg
    10081 cgtaatattc gtagatgata ggagcggtgt actcgatctt atccgcgatc tttcgcatgc
    10141 tcagcgcctg ccagccttcg tctttcacga tgtcgtaagc tgcgtccagg atgtttaccc
    10201 tggtctgttc cttcaatcga tggattctct ccttgcttcc catgcttttt acccccttat
    10261 gcacccgtta ccctcttatg cacccattcg ggcctcgggc tttaccctcg gtaaatcatt
    10321 taacgctgta aaattaggga atgttcagta tcggcaaaaa tttttttttt gccacaccgc
    10381 cgcgccggcc acaccgctgc cgcgccggcc acaccgccac cgtgccggct gcattactcc
    10441 gtccgcagac tatccaccgg attggcgtag gccgccttaa tagcctgaaa gctaatcgtc
    10501 agcatggcta tcagcacggc agtcagaccc gccgcgagga agatccacca ggccgaatag
    10561 atatttatcc gtgaggcgaa accatccacc caacgcttca tcagccagaa agaaagcggt
    10621 gtcgcgacga cgaaagcgat cccgatcagc accaggaaat ccctggataa tagccgtacg
    10681 atgcctgtta cgctggctcc gagcaccttc cggacgccga tctccttggt cctttgcatg
    10741 gtactataag aggccagtcc gagcagccca agacaggaga tcaggatggc tagcacggaa
    10801 aaatagagga acagttttcc aaaccggtct tcggcgcggt attgccggtc gaacatatcg
    10861 tccacgaaga aatagctgaa cgggcggctg ggaatgaccg tatgccaggt cttctcgatc
    10921 gcggcgatcg tgttcttcaa attggaggtg ctcaccttca ccgaaagcat gttacagcca
    10981 tccggctcga tccgcatgct cagcggccgg atctcctcct gtaagccctg gtagtgaaaa
    11041 tctttgacca ccccgatg
//
LOCUS       DASYSV010000536         3812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1393312, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000536 DASYSV010000000
VERSION     DASYSV010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393312"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="VGQ51_16765"
     CDS             <1..1939
                     /locus_tag="VGQ51_16765"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEV9038291.1"
                     /translation="LDETRSAVYMDNTRNFPKNTEFEALVTFGGGGAGGGGFRRGGAV
                     APDPTAVTVRIHQAFVQLPDSGFRSRKFDPRSGFFPFSYYDFSADMTEPLMKRFIERH
                     RLIKKDPNAAVSEPVEPIIYYIDRGAPPIIKKALIEGGSWWNQAFEAAGFKNAFFVKE
                     LPEGADPMDIRYNVVNWLDRSGSPQRAFSYGASYIDPRTGEIIKGMVTLGSDRHRQDY
                     LIAEGLLQPYTDGKPVDPRMQEMALARIRQLSAHEIGHTLGLEHNFEASVKDRASVMD
                     YPFPRMTLKSDGTIDLSDAYAKGIGGWDKRAIMWGYSDFPKGTDEDAALEKIMKETLK
                     EGYKYIPDIGGMAHPMSNQWDDGVNPIDQLEKLMAIRRRLLDNFSEKAIPKDAPMATL
                     EEVLVPIYLLHRYQVEAVAKSLGGLYFTHAVKNDGEEPTKMVEPAEQWRAFDALMNTI
                     TPEALVLPETLIQKIPPRPTGYPSTIEIFRGHTGPTFDPIAVAEAAAENTLSYLLNSE
                     RAARLIEYQARDERQPGLLPVLDKLIDKTWKTSLQPGYKGELQLLVDNLTLKHLLSLA
                     ADPRNAENVRGEALLTINGLKDWINGQLGGAKGKWKGALYFGLSQIEEFSKDPNKFVA
                     EPAVEMPPGAPIGMPDDEFAF"
     gene            complement(2227..>3812)
                     /locus_tag="VGQ51_16770"
     CDS             complement(2227..>3812)
                     /locus_tag="VGQ51_16770"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9038292.1"
                     /translation="HERATVVYYSKDTIYVGTLNGLYRSANGQPPVFLGDQDPFLRRR
                     ISSIAESADGTLWISSYDAGIIGFKDGRQTSSITSRQGLTSDFCRNLVVNGNTLWAGT
                     DKGLNRIRLDRPDHPVIQYTSRDGLASDVINAVCIDSPVVYVGTPEGLNYFDENKMAS
                     QSHCLLYLMSVMNDDRDRISDTSHLVIPYTDRRIRFEFAGIDYKSAGDITYRYRMSGL
                     DEKWIETKQNVLEYPDMPSGNYTFQLQAIDRFGVHSRLMTVPLEVTAPLSKRAWFIVT
                     AWLASLGLLWMLVSLRVQRIRRRQQEKERLTREMNQLKNTALKSQMNPHFIFNCLNSI
                     QHSIFTGDTDAANSYISGLARLIRMTLNDSSRSFVSIEDEADYLGSYLQLEKMRFKEK
                     IGYEIGIDPTIDRSAVLIPPMLIQPYVENALHHGLGPRPEGKGRVTIRIDRKGDRLIV
                     VVEDNGVGRASAEHRITTGEHLSKGMSLTEGRLEILSRLYERPFNISVVDLKDDRDRP
                     AGTRIVIDLPLFLETSLYT"
BASE COUNT          838 a          969 c         1191 g          814 t
ORIGIN      
        1 gctggatgag acgcgttcgg ctgtctatat ggataatacc cggaactttc ctaagaatac
       61 ggagtttgaa gcgctggtga cgtttggcgg cggcggcgca ggtggtggcg ggttcagaag
      121 gggcggagcg gttgcaccgg atccgaccgc ggttacggtg aggatacatc aggccttcgt
      181 acaactgccc gacagcggct tccggtcgcg gaaattcgat ccgagatcgg ggttctttcc
      241 cttcagctat tatgattttt ccgccgacat gaccgagccg ctgatgaagc ggttcatcga
      301 gcggcaccgg cttatcaaga aagacccgaa tgctgcggtc agcgagccgg tggaacccat
      361 catctactac atcgacaggg gcgcaccgcc catcatcaag aaagcgctga tcgaaggcgg
      421 ctcgtggtgg aaccaggctt tcgaggccgc gggtttcaag aacgcctttt ttgtcaaaga
      481 gctgcccgag ggcgccgatc cgatggatat ccgttataat gttgtcaact ggctggacag
      541 aagcggcagt ccgcaaaggg ccttttcata tggcgccagc tatatcgatc cgcgcacggg
      601 cgagatcatc aagggcatgg tgacattggg ctcggaccgc catcgtcagg actatctgat
      661 cgccgagggt ctgcttcaac cctatacaga cggcaagccg gtggacccgc gtatgcagga
      721 gatggcgctg gcgcgtatcc gtcagctgtc cgcgcatgag atcggtcata cgctggggtt
      781 ggaacataat ttcgaggcca gcgtgaagga ccgcgcatcg gtaatggatt accctttccc
      841 ccggatgacg ctaaagtccg acgggacgat cgatctctcc gacgcctatg cgaagggcat
      901 cggcggctgg gacaaacgtg cgatcatgtg gggttactcc gacttcccaa aagggacgga
      961 tgaagacgct gcgctggaaa agatcatgaa agagactttg aaagaaggct ataaatacat
     1021 ccccgatatc ggtggcatgg cgcatccgat gtccaatcaa tgggacgacg gggtgaaccc
     1081 aatcgatcag ctggaaaagc tgatggcgat ccgtcgacgg ctgctggaca atttctccga
     1141 gaaggccata ccgaaggatg cgcctatggc gacactcgag gaagtgctgg tccccatcta
     1201 cctcctgcac cgttaccagg tggaggctgt cgccaaatcg ctgggtggac tttactttac
     1261 gcatgccgtt aaaaatgacg gtgaagagcc cacgaagatg gtagagcctg ccgagcaatg
     1321 gagggctttc gacgccctga tgaatacgat caccccggag gctctcgtgc tgccggagac
     1381 attgatccaa aaaataccgc cgcgtcccac gggctatccg tcgaccatcg agatcttccg
     1441 cggacatacg ggtccgacct tcgaccccat cgctgtagcg gaagcagccg cggagaacac
     1501 gctgtcctat ctgctgaata gcgaacgggc cgccagactc atcgaatacc aggcgcggga
     1561 cgaacgtcag cccggtcttt tacccgtgct ggacaagctg atcgacaaga cgtggaaaac
     1621 gtcgttgcag ccgggttata aaggggagct ccagttgctg gtggacaacc tgacgctgaa
     1681 acacctgttg tcgctggccg cggacccgcg caatgcggaa aatgtacggg gtgaggcgct
     1741 gctgacgatc aatgggctga aggattggat caatggacag ctgggcgggg cgaaggggaa
     1801 atggaagggg gcgttgtatt tcggtctctc tcagatagaa gaattttcga aggacccgaa
     1861 taaattcgtg gccgagccgg cggtggagat gcctccgggc gcgccgatag ggatgccgga
     1921 tgatgaattt gcgttttagg ggggagggag gctttcgcgg ggaggctcgg gcgggggctg
     1981 aatcggcgtg aagttgcgcg ggaggctcgg gcggggggca aaaaaacacc ccccggctta
     2041 accggggggg tattaggagt aaggatatga tcaatggcct attgtgatct gttagggtaa
     2101 acggccaggg ttaaacgggc atggctggaa taaatgtacg gttgttcccg atgcggccag
     2161 gtaagagttc accggcgtgt cgattttggc aataagtgtg caattgggcg gataagtgtg
     2221 caggcgtcag gtatagaggc tggtttccag gaacagcggc agatcgatga caatacgggt
     2281 gccggccggc cggtcgcggt catctttcag atcgactacc gaaatgttga aagggcgttc
     2341 atacagccgg ctcaggatct cgagacggcc ttcggtcagc gacatccctt tggaaagatg
     2401 ctccccggtc gtgatccggt gttcggccga ggcccttccc acaccattgt cttccaccac
     2461 tacgatcagc ctgtcgcctt tcctgtcgat gcggatggtg acgcggcctt taccttccgg
     2521 cctggggccc aaaccgtggt gaagggcgtt ctcgacataa ggttgtatga gcatgggggg
     2581 tatgagcacg gccgaccggt cgatggtggg atcgatcccg atctcgtagc cgatcttctc
     2641 tttgaaacgc atcttttcca gctggagata ggagccgaga taatcggcct cgtcttcgat
     2701 ggacacgaac gagcgggagg aatcgttcag ggtcatccgg atcagtctgg cgagaccgga
     2761 gatatagctg ttggcggcgt cggtatcacc ggtaaagatg gagtgttgaa tcgaattgag
     2821 acaattgaaa atgaaatgcg ggttcatctg cgatttcagc gccgtattct tcagctgatt
     2881 catctcgcgg gtaagccgct ctttttcctg ctgccggcgg cggatgcgtt ggacccttag
     2941 cgagaccagc atccaaagca gacccagtga tgccagccag gcggtgacaa tgaaccaggc
     3001 gcgtttcgat agcggcgcgg tgacttcgag gggcaccgtc atcagcctgc tgtgtacgcc
     3061 gaaccgatcg atcgcctgca attggaaggt atagtttccc gaaggcatat cgggatattc
     3121 cagcacattt tgttttgttt cgatccattt ttcatccagc cccgacatcc ggtagcggta
     3181 agtaatatcc ccggcagact tataatcgat gccggcgaat tcgaaacgga tacgtctatc
     3241 cgtataggga atgacgagat gcgacgtgtc ggaaatgcga tcgcggtcat cgttcatcac
     3301 cgacatcagg tatagcagac agtgactttg gctggccatt ttgttctcat cgaagtaatt
     3361 gagtccttca ggggtgccga catagacgac cgggctgtcg atacagacgg cgttgatcac
     3421 atccgatgcc agaccgtcgc gggaggtata ttgaataacg ggatgatccg gtctgtcgag
     3481 acggatcctg ttaagtcctt tgtcagtgcc ggcccagagc gtgttcccat tcaccacgag
     3541 gttcctgcag aaatcgctgg tcagaccctg gcggctggtg atcgagctcg tttgtcttcc
     3601 gtctttgaac ccgatgatcc cggcatcata ggaggagatc cagagagtac catctgcgga
     3661 ttcggcgatg gaggatatcc gtcttcgcag aaaagggtcc tgatcgccga ggaatacggg
     3721 gggctgtcca tttgccgagc ggtagaggcc attgagcgtt ccgacgtaga tggtgtcctt
     3781 gctgtaatag accactgttg cgcgttcgtg cc
//
LOCUS       DASYSV010000537         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1135949, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000537 DASYSV010000000
VERSION     DASYSV010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135949"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VGQ51_16775"
     CDS             complement(<1..1462)
                     /locus_tag="VGQ51_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEV9038293.1"
                     /translation="MKCPHKIQYILLILPVLLAHSPARAQYDPDKVNKKAVILYNKAL
                     QQAAGDDDLKAGIATLEQAIRIDKNYEEAYLSMAGMYGEMKNYHAAIDNYEKAMAIDS
                     NFFLDYNLPYSIDLAGIGNFDMALQAIGRFLTIPNLNETSRKAGEYRRGCYRFAIDYA
                     AAHPNANYKFEPQNLGDSLNTGDSEYYPSISLDGDTLFFTRRVHHTNEDLFEAYRLPG
                     GGWTKAHGLPGNINTNENEGAQTVSIDGQWMILDICNSEEGRGGCDLYISYRTPDGWS
                     APENLGDSINTEFRETGASLSPDKKDLYFSSNRPGGYGGSDLYVSHHLLNGRWSGAEN
                     LGPVLNTIGDESTPFIHVDNQTLYFNSTGHPGYGQNDLFVSRKTADGGWSKPENLGYP
                     INTIDDEGSLVIASDAKTAYYASDRSDSRGGMDLYSFILRDDIRPAQTLWVKGRVFDK
                     KTGKGLPSSVILTDLSTGRVISALMTDETGNYLTTLP"
     gene            complement(1501..2106)
                     /locus_tag="VGQ51_16780"
     CDS             complement(1501..2106)
                     /locus_tag="VGQ51_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEV9038294.1"
                     /translation="MATDSLPVMESFYTLQGEGYHQGKAAYFIRLAGCDVGCVWCDVK
                     DSWDAGRRPRRSIADLVTEASAHPGRIAVITGGEPLMHDCTGLTDALHRAGFATHIET
                     SGAWPLSGSWDWICLSPKKFKAPLPDIPPVASELKIVIYNQSDFAWAEQWAALASPGC
                     RLFLQPEWSKSPTVTPLIIDYIKQHPNWEFSLQLHKYIHVP"
     gene            complement(2096..2977)
                     /gene="folD"
                     /locus_tag="VGQ51_16785"
     CDS             complement(2096..2977)
                     /gene="folD"
                     /locus_tag="VGQ51_16785"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEV9038295.1"
                     /translation="MELLDGKLASQAIKDSLKLKVAQIITEGKKVPHLAAVLVGNSGA
                     SETYVGAKVKACAEIGYRSTLIRLEDSISENRLLSVIEELNNDMDVDGILVQLPLPGH
                     ISDEKVITAIHPSKDVDGFHPENVGRLVLGQPTFISATPHGILLLLEHYKIDTRGMHA
                     VVVGRSNIVGRPMSILLSANSNPGNCTVTVCHSRTRNLEEICQQADLIVAALGKPEFV
                     TASMVKKGAIVIDVGITRIADPAKKSGFALKGDVKFDEVAPLCSYITPVPGGVGPMTI
                     AALMKNTFKACELRNGN"
     gene            3497..>4003
                     /locus_tag="VGQ51_16790"
     CDS             3497..>4003
                     /locus_tag="VGQ51_16790"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9038296.1"
                     /translation="MRKCLSLLAVLLLSMGCVFAQQKTITGRVVDAQNQPVPFASVRV
                     KSSKAGVAADADGNFSIKAKVGDILVVSAVTGAAPAEVTVGSDNSITVTLQRTKSNME
                     EVVVTALGVTKSRNRVAYAAQTISGSDVSDNRSFDIAQSMSGKVAGVQISQSNTLGGS
                     TNVILRGYK"
BASE COUNT          902 a          966 c         1094 g         1041 t
ORIGIN      
        1 tgggaagggt agtcagataa ttgccggtct cgtccgtcat cagcgcactg atcaccctgc
       61 ccgttgacag gtcggtgagg attacactgg aaggcagccc cttgccggtc ttcttatcga
      121 atacgcgtcc cttaacccag agcgtctgtg ccggacggat atcgtcgcgc aggataaagg
      181 aatagaggtc catgcctccg cggctgtcgg acctgtcgct ggcataataa gccgttttgg
      241 cgtcggaggc gatgaccagg ctgccctcgt catcgatagt attgatcggg tatcccaggt
      301 tctccggttt gctccatccc ccgtccgccg ttttcctgga aacgaacagg tcgttttgtc
      361 catagccggg atgtccggtg gagttgaaat acaaagtttg gttatcgaca tgtatgaagg
      421 gagtggattc gtctccgatg gtattgagca ctggacccag gttctcggcg ccgctccatc
      481 gtccgttgag caggtggtgg ctgacgtaaa gatcgctgcc gccgtagcct ccgggtctgt
      541 tgctcgaaaa atacaggtcc ttcttatcgg gcgaaagcga ggcgccggtc tcacggaatt
      601 cggtattgat actgtcgccg aggttctccg gcgcgctcca gccgtctggt gtgcgatagg
      661 aaatatagag gtcgcagcct ccgcgacctt cctcggaatt gcagatgtcg aggatcatcc
      721 attgaccgtc tatcgatacc gtttgcgccc cttcgttttc gttcgtattg atattgccgg
      781 gcagcccatg ggccttggtc catccgcctc cggggaggcg gtatgcttcg aagaggtctt
      841 cattggtatg atggacccgc cgggtaaaaa atagggtatc accgtcgagg ctgatgctgg
      901 gataatattc ggaatcgccg gtattgaggc tatcccccag gttttgcggt tcgaatttat
      961 aattggcgtt gggatgagcg gccgcatagt ctatggcaaa tcggtagcag ccacggcggt
     1021 attctccagc cttccggctt gtttcgttga gattggggat agtcaggaat cggccgatcg
     1081 cctgcagcgc catgtcaaaa tttccgatgc cggccagatc gatcgagtag gggaggttat
     1141 aatccagaaa gaaattacta tcgatggcca tggccttttc gtagttgtcg atggcggcgt
     1201 gataattttt catttcccca tacatgccgg ccatggagag ataggcttct tcatagttct
     1261 tgtcgatgcg gatcgcctgt tccagcgtag cgatgcctgc tttcagatcg tcatccccag
     1321 cagcctgctg gagcgctttg ttatagagta ttaccgcttt tttgttgacc ttgtccggat
     1381 cgtactgtgc ccgggcaggt gaatgggcca gaaggaccgg aagaataagg aggatgtatt
     1441 ggattttatg cggacatttc atggctgaaa attaataaat ttcccgcata gggaggaaat
     1501 tcagggcaca tggatgtatt tatgcaattg aagcgagaat tcccaattcg ggtgctgctt
     1561 gatgtaatcg atgatgagcg gagtgacggt gggagatttg ctccattcgg gttggaggaa
     1621 aagacggcaa ccgggtgagg ccagggctgc ccattgttcg gcccaggcga agtccgactg
     1681 gttgtagatg acgatcttca attcgctggc aacgggaggg atatcaggta gcggcgcctt
     1741 gaattttttg ggtgaaagac agatccagtc ccaactgccg ctgagtggcc aggcgcccga
     1801 ggtctcgata tgcgtggcga atccggcacg gtgcagcgca tcggtgagcc cggtacagtc
     1861 gtgcatcaaa ggttcacccc ccgtgattac ggcgatcctc cccggatggg cggaggcttc
     1921 ggttaccaga tcggcaatac tgcgacgggg acgccggccg gcgtcccaac tgtccttgac
     1981 atcgcaccag acacaaccca catcgcatcc tgccagtctt atgaaataag cggccttacc
     2041 ctggtggtaa ccttcaccct gaagggtata gaaggattcc attaccggaa gtgaatcagt
     2101 tgccatttcg gagttcacag gctttgaagg tatttttcat caatgcggca atggtcatgg
     2161 gaccgacgcc cccgggtacc ggagtgatat agctgcaaag tggcgctacc tcatcgaatt
     2221 tgacatctcc tttgagggcg aaaccgcttt ttttggcggg atcggcaatg cgggtaatac
     2281 cgacatcgat gactattgcg ccttttttaa ccatggaggc ggtgacgaat tcgggtttgc
     2341 cgagcgcggc cacgatcaga tcggcctgct gacatatttc ttccaggttg cgggtccgcg
     2401 aatgacagac ggtgaccgtg cagttgccgg ggttggaatt ggcgctcagg aggatgctca
     2461 tgggtctgcc tacgatattg ctgcgcccga cgaccacggc atgcatcccg cgggtgtcga
     2521 tcttataatg ctcgagcagc agcaggatac catgaggagt ggcagagatg aaggtcggct
     2581 gtcccaggac gagtcttcct acgttttcgg gatggaatcc atccacatcc ttggagggat
     2641 ggatggcagt gatgaccttt tcatcggaga tatgcccggg cagcggcaac tgtaccagga
     2701 tgccatctac atccatatcg ttattgagct cctcgattac ggaaagcagc cggttctcgc
     2761 tgatgctgtc ttccagccgg ataagcgtcg agcggtaacc gatctccgcg caggctttca
     2821 cttttgcccc cacataggtt tcgctggccc cgctgttgcc gaccagcact gcggccagat
     2881 gtggtacctt ttttccttcc gttatgatct gcgccacctt tagttttagc gaatccttga
     2941 tggcctgcga ggccagtttg ccgtctaata attccatcgc gcaaaggtag tgtttatgaa
     3001 aaggaaaaac gaatgctctc atactgcagg gagcgggagc ggaacaacca atcgttaaca
     3061 ggaagtcgga ctgattcttt atttttggca gaagtcattt gtttagtaat ttagttccgt
     3121 caatactcca gagaaatcaa aactgagtca catgagaatt ctttccctga ccgaatgggg
     3181 gggcagtctg atctaccatt gcctcaagcg tatagaggtc ctcttcagct ttttcagcat
     3241 tttatttatt atcattattt tgaatacaac atgcagcatt cgctgaagtt tgtttgtcca
     3301 ttcagatatt tttaacaaca ataaaacaga gtcacatgag aaaattacct ccaccacttt
     3361 ttcatttgtc gggataatcc ctttctaatt cttttttttc gccggatccg agtcgagatc
     3421 caatccaaaa attattgttt atcaatcatt tgtcaataag gcagtatggt ctgcctcaaa
     3481 aactaaaact tttcaaatga gaaaatgtct atcactgttg gcggtgctgc tcctctccat
     3541 ggggtgtgta ttcgcacaac aaaaaaccat cacaggtaga gtcgtcgatg cccaaaatca
     3601 acctgttcca tttgcatctg tcagagtcaa atccagcaag gcaggagtag ctgccgatgc
     3661 ggatggtaat tttagcatca aagcgaaagt tggggatatt ttggttgtgt cggcggtgac
     3721 cggagcagct cctgccgaag ttacagttgg ttccgataat tctatcactg ttacattgca
     3781 acgtaccaag agtaatatgg aggaagtcgt tgttacagct cttggcgtta cgaaaagccg
     3841 gaatcgtgtc gcctacgcgg cccaaacaat ttccggttct gatgtaagcg ataacagaag
     3901 ttttgacatc gctcaatcca tgtcaggtaa ggttgccggt gtacagatct ctcaaagcaa
     3961 tactttggga ggttccacga atgtgatctt aagaggatat aag
//
LOCUS       DASYSV010000538         4172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2289187, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000538 DASYSV010000000
VERSION     DASYSV010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289187"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..628)
                     /locus_tag="VGQ51_16795"
     CDS             complement(281..628)
                     /locus_tag="VGQ51_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEV9038297.1"
                     /translation="MAIKITEECINCGACEPECPNNAIYEGGVEWAITDGTTVKGSFT
                     LLDGSAIDASQRNAPISVDTYYIVPNKCTECQGFHEEPQCAAVCPVDCCVPDEMYRET
                     VEELLAKKERLHI"
     gene            729..1739
                     /locus_tag="VGQ51_16800"
     CDS             729..1739
                     /locus_tag="VGQ51_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEV9038298.1"
                     /translation="MKLEERIDLLTRLGDYMLSDDPAWEAARRKAQAVNGWFIQEFVS
                     RAVEQIAAGYLQRESLREWGERYGLPSERTHPLTVGLVMAGNIPLVGFHDFLSVFMSG
                     HRQLIKPSKQDDVLIRHLADHLKTVDPRVGDYVGFAERLNGCDAYIATGSDNSARYFE
                     YYFGKYPHIIRRNRTSVAVLTGEETRGELEGLADDVYCYFGLGCRNVTQLLVPEGYDF
                     LPLLEAFRKYNYLFDLAKYKHNYDYQLTLLILNKKYYMTNGSILLTENESPFAAISVL
                     HYRYYPAGGPVLAGGPMPGGDPTQIQSVVGPGHLPFGKAQHPGLQDYADGVDTMAFLQ
                     RL"
     gene            1942..3459
                     /locus_tag="VGQ51_16805"
     CDS             1942..3459
                     /locus_tag="VGQ51_16805"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEV9038299.1"
                     /translation="MSAKQVFAVVLISASTAVASVWGYNRFTSGKTYYYSQDSGKVPA
                     NYAKFFEGKYKDGAGPNDFVDAASAAIPATVHIKTKTVRTVSNNLPRHNPFSDLFGID
                     PDDFFGNGNGMRSLPEMASGSGVIISNDGYIVTNNHVVDGADEVTVTLSNRKSFKAKL
                     IGADPSSDLAVVKIDAQNLPFLLYGNSDEVKVGQWVLAVGYPLTLETTVTAGIVSAKG
                     RTLDINRRQSQSPVESFIQTDAAVNPGNSGGPLINTSGQLVGINSAIASPTGSYAGYS
                     FTIPVNIVKKVVNDIIKFGTSQRAYLGISYPRDDMSEDQKKENGIKDGDGVYVWTISP
                     DGPAAHAGIKQGDIITRIDGEDVTTGAEMVGKIATFSPGDKIKVTYKRDGKENSVSVT
                     LSNNSSSTSTSVIKSGVLDKLGAQLENLSKKDAASLDVKGGVVVKSITENGLMDKSRI
                     QEGFIILKANGEDVKNVDDLQQILNKAGNGTVKLEGVFPGYEGVYSYPLRLASGG"
     gene            complement(3498..>4172)
                     /locus_tag="VGQ51_16810"
     CDS             complement(3498..>4172)
                     /locus_tag="VGQ51_16810"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV9038300.1"
                     /translation="EEILYHEVFDATYTWKLLHRMEAVWRKESPLGGIDEVLDEYDTQ
                     FPPNGLRLYFTTNHDENSHSGSEFERMGDMAKCFAVLACTWNGIPLIYSGQEMPNYKR
                     LKFFDKDSIEWTGRYELHGFFRTLLWLRKSNPALRSGDPLVTIHRLRTQFDERAFIFA
                     RRSDEDQVVVVLNLSPEPLQLPVGELLLKGIYREVFTGFWFDLSRVPTLYIPPWGYNV
                     LEKQKG"
BASE COUNT         1022 a         1161 c         1063 g          926 t
ORIGIN      
        1 cgaaaaagtt ttcgcaaaat aacgaaaact ctgccggtcg cagtacgcac aaaataagaa
       61 aggtgaagga atggcccctt caccctttta tgacgggaaa tatcacccgc ctattatcac
      121 tgtagcacga agctacatgt acaaacaaaa tactaacccg aaggtaccga ttttaatgaa
      181 aattcccaat tacaagtcca cgagttttcc acctttttcg cagcagagat tgctcagctc
      241 accgcccaac gcctcgcgca gcctctgcat ccgcgaccca ctaaatatgc agccgctctt
      301 tcttcgccaa cagctcttcg accgtctcgc ggtacatctc atcgggcacg cagcagtcta
      361 cggggcaaac cgccgcacat tgcggttctt catgaaatcc ctgacattcg gtgcatttat
      421 tcgggacgat ataatacgta tccacgctga taggcgcatt gcgctgggac gcatcgatcg
      481 ccgatccgtc cagaagggtg aaagatccct tgaccgtcgt tccatcggtg atcgcccatt
      541 ctacgccgcc ctcatagatt gcattattcg gacattccgg ttcgcaggcg ccgcaattga
      601 tgcattcttc tgtgatctta atagccattt ttttacaaaa atttggaggt gattaaagtt
      661 acctttgcgt ttccgccaag gactcacggg gccgatgccc ccttagcaaa tataaaggat
      721 ttgattttat gaagctggag gagcgtatcg atttattgac ccggctaggg gattacatgt
      781 tgtcggacga ccctgcctgg gaagccgccc gccggaaggc gcaagcggtc aacggatggt
      841 tcatccagga gtttgttagc agggccgtgg aacagatcgc cgccggctat ctgcaacgcg
      901 aaagcctccg cgaatggggc gaaagatacg gcctgccctc tgaaaggacc cacccgctga
      961 cggtgggcct ggtcatggcc ggcaatatcc cccttgtcgg atttcatgat ttcctttcgg
     1021 tattcatgtc cggacaccgc cagctgatca aaccttccaa acaggatgac gtgctcatcc
     1081 ggcacctggc ggaccacctc aaaacagtcg accccagggt cggggattac gttgggtttg
     1141 ccgaaaggct aaatggctgc gatgcctata tcgcaaccgg cagcgacaat tctgcccggt
     1201 atttcgaata ttatttcgga aaatatcctc atatcatccg gcgcaaccgt acctcggtgg
     1261 ccgtactcac cggtgaggag accaggggtg aactcgaagg ccttgccgat gacgtttatt
     1321 gctatttcgg cctggggtgc cgcaatgtca cccagctgct ggtaccggaa ggttacgatt
     1381 tcctgcccct cctcgaagcc ttccggaaat acaactatct cttcgatctc gccaaataca
     1441 aacataatta cgattatcag ctgactctcc tgatcctgaa taaaaagtac tatatgacca
     1501 acgggtctat cctgttgaca gagaatgagt ctccgttcgc tgctattagc gtactacact
     1561 atcggtatta tccggcgggc gggccggtgc ttgcgggcgg gccgatgccg ggaggcgacc
     1621 caacccagat ccaatccgtg gtagggccgg gccatctacc attcggaaaa gcccagcatc
     1681 ccggcctgca ggactatgcc gatggtgtcg ataccatggc cttcctccag cgtttgtaaa
     1741 ttttggcaga cggcgtccag ggctgccgga cggaccagta attctggcgt tttttccgta
     1801 cctttaatac aacggttccg acctacgaag gtcttagtaa attatttgtt aaactccttc
     1861 gttccctggt gtagaagagt ggtcttgccg ttattttgtc atcgcgacaa atgataccct
     1921 taacgaaaaa aagattatcc aatgagcgca aaacaagtat tcgccgtcgt cctcatcagc
     1981 gctagcactg cggtggcttc ggtctggggc tataaccgat tcacctcggg taagacgtac
     2041 tactattccc aggattccgg caaggttccg gccaattatg ccaaattctt cgaaggcaaa
     2101 tacaaggatg gagcagggcc caacgacttc gtcgatgccg ccagcgcggc cataccggcc
     2161 acagttcaca tcaagacaaa gaccgtcagg acggtcagca acaatctgcc ccggcacaat
     2221 ccgttctccg acctgttcgg tatcgacccc gacgatttct tcggcaatgg caacgggatg
     2281 cgctccctgc cggagatggc ttccggttcc ggggtgatca tcagcaatga cgggtacatc
     2341 gtcaccaaca accacgtcgt agacggcgcc gatgaggtga ccgtaaccct cagcaaccgg
     2401 aaatctttca aagccaagct catcggcgcc gatcccagca gcgatctggc cgtcgtgaag
     2461 atcgacgcgc agaatctgcc cttcctcctc tatggcaaca gcgatgaggt caaggtcggt
     2521 caatgggtgc tggctgtagg ataccccctg acactggaaa cgaccgttac ggcaggtatc
     2581 gtcagtgcca agggaaggac cctggacatc aacaggcgtc aaagccagtc tccggtggaa
     2641 tcctttatcc agacggacgc ggcggtcaat cccggcaaca gcggcggacc gctgatcaat
     2701 acctcaggac agctggtcgg catcaactcc gccatcgcct cgcccacagg ttcatacgcc
     2761 ggctactcct ttaccattcc ggtcaatatt gtcaaaaagg tggtgaacga tatcattaaa
     2821 ttcgggactt cgcaacgtgc atacctgggt atctcctatc cccgcgacga tatgagtgaa
     2881 gaccagaaaa aggaaaatgg aattaaagat ggcgacggcg tttatgtttg gacgataagc
     2941 ccggacggac cggcggcgca tgcagggatc aagcaaggcg atatcattac caggatcgac
     3001 ggtgaagacg ttactaccgg cgccgaaatg gtcggtaaga tcgctacttt cagccccggc
     3061 gacaagatca aggtgaccta caaacgcgac ggaaaggaga actctgtttc cgtcaccctg
     3121 agcaataatt ccagcagtac ctctacctcg gtcatcaaat ccggcgtgct ggacaagctg
     3181 ggcgcccaac tggaaaatct cagcaagaag gatgcggcca gtctcgacgt aaaaggtggg
     3241 gtagtcgtca aatccattac cgaaaacggc cttatggaca aatcacgcat ccaggaaggg
     3301 ttcatcatcc tcaaagccaa tggcgaagat gtgaagaatg tcgatgatct ccagcaaata
     3361 ctgaacaagg cgggcaatgg gaccgtcaag ctggaaggag tcttccccgg atatgaaggc
     3421 gtttactcgt atccgttgcg tctggcaagc ggcggatagt ggcccccggt atctaattta
     3481 aaaaggctga ccgatggtca gcctttttgt ttctccagca cattatagcc ccagggcggt
     3541 atatacagcg tgggaacgcg tgaaagatcg aaccagaaac ctgtgaatac ctcgcggtag
     3601 atgcccttca gcaatagttc gccaacgggc agctgcaatg gctccgggga tagattcagg
     3661 acaacgacta cctggtcctc gtccgaacgt ctcgcgaaaa tgaacgcccg ttcatcgaac
     3721 tgtgtacgca gccggtggat cgtaaccaag ggatccccgc tgcgaagcgc ggggttgctt
     3781 tttctcagcc aaagcaaagt ccggaaaaaa ccgtgcagtt cataacgccc cgtccattcg
     3841 atggagtcct tatcgaaaaa cttcaaccgt ttgtaattcg gcatctcctg cccgctatag
     3901 atcagcggta tgccgttcca ggtgcaggcc agtacggcaa aacatttcgc catgtcgccc
     3961 atccgttcga attcgctgcc gctgtgcgaa ttttcgtcat ggttggttgt aaaatacagc
     4021 cggagcccgt tgggagggaa ttgcgtgtca tattcatcga ggacctcgtc gatgccaccg
     4081 agtggcgact ccttcctcca tacagcttcc atccggtgca gcaatttcca ggtataggtg
     4141 gcgtcgaaga cctcatggta gagtatctcc tc
//
LOCUS       DASYSV010000539         5119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2416605, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000539 DASYSV010000000
VERSION     DASYSV010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416605"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="VGQ51_16815"
     CDS             <1..1498
                     /locus_tag="VGQ51_16815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9038301.1"
                     /translation="SGSSEAIPEAEEKAQPTDGDTAIVHQRNQQLEKELASLREDMRN
                     FAEDQEAVIEELQSANEELLSGSEELQSLNEELETSKEEIQSTNEELTTLNQELFDRN
                     EQLNLSRLYAESIVATIREPLLVLDHNMQVKTANRAFYEKFGASEEETEGKSLYGLGR
                     GEWDTPTLRAALGSILSREASIKDIEINYSVNAGGERILLLNASRIFRKDSAEQLVLL
                     AMEDITEATKRENEQRRFADELAKQVDERTASLKEANTALKYSNESLEQFATIASHDL
                     QEPLRKIRTFATILNNEHSADIDEETRGLLQKINLSAERMAALIRDVLNFSKVLDASV
                     FEATDLTLILQSVIRDFDLQIEEKQAVIRYDPLPSVQAVPLQMNQLLYNLLGNALKFS
                     KTGLPPVIDISSRTLPPDELKEHPQLDNRLPYCEIVFADQGIGIDSRFADRIFLIFQR
                     LNTRQHFEGTGIGLALCKRIVTNHGGEIYFQPNEGGGARFHVILPLVH"
     gene            complement(1495..2535)
                     /locus_tag="VGQ51_16820"
     CDS             complement(1495..2535)
                     /locus_tag="VGQ51_16820"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEV9038302.1"
                     /translation="MDYVLRVPSYGWMDAEGGLVVPSTRQIMNEFWARLNIFRTKKNW
                     LAFLNWTKVLLLIPCLFIFLLHDFSFPLLGVAFIYSMIIMGTHGTIWYHRYCTHHAYA
                     FSNRFWRFVTRNLTINIVPEETYVISHHVHHAKSDRPGDPYNASAGFLYCFLADVNHQ
                     AISEHLTEHDYTRVVKMMAGSGIKSNTYRQYLQWGSVVRPAWHLVGWILNWSFWGVVF
                     YLAGGEALVFALFGAAGFWAIGIRTFNYEGHGKGKDKHREGIDFNRKDLSINQLWPGY
                     VAGEWHNNHHLFPSSARSGFLNYQLDLAWCYIRFLKVIGAVRSYHDSKKAFLLQYREP
                     FLKRKLERGKPV"
     gene            2749..3822
                     /locus_tag="VGQ51_16825"
     CDS             2749..3822
                     /locus_tag="VGQ51_16825"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEV9038303.1"
                     /translation="MPKITFNNSNNTFFQSVKNSADAYFKTQKLKRTGNWKLYLKAWI
                     LIPASLGIYLFLLSGHYTWLMGILASVFLGFTLVCIAFNVMHDACHGSFSEKKWVNNI
                     MGLTMNALGSNAFIWKIKHNVIHHTYTNIDKIDDDIANGPLLRQCTTQKWMPIHRFQF
                     IYMFLLYGVSTLSWALGTDFARYFRKKIHHTPINRIGVKQHLLFWASKLLYAFFYALL
                     PIYVLGWQPWLIGFLVVNITMGLSLSVVFQLAHIVEKTTFDVADGTHKVIGSEWAIHE
                     IRTTADFAPGNRIISWLVGGLNFQIEHHLFPQVSHIHYAALSKIVRQQCELFGLPYNY
                     YPTAWQAVYSHVRLMRRLGTGIR"
     gene            3841..4278
                     /locus_tag="VGQ51_16830"
     CDS             3841..4278
                     /locus_tag="VGQ51_16830"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEV9038304.1"
                     /translation="MKDHDLANGETGATPDNLMDDDRTGTNHGELYSNSPLKYLTASS
                     IIGDKVHNETDEQMGEIMDIMIDVVTGKIDYFVVEFGGFFGIGVKYFAIPFRLLRVDP
                     ENKRFIFNQDKKTLEQAPGFDTDHWPHTNIHLDQVYSYWSFMG"
     gene            complement(4311..4790)
                     /locus_tag="VGQ51_16835"
     CDS             complement(4311..4790)
                     /locus_tag="VGQ51_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038305.1"
                     /translation="MKKVFYLIPAGLLFFSASVTAQQANIDSLKLVAQISQDQLQLGK
                     LQNMVAQKTKDQEGAALNAQNSASDNANAAQRLNDDPTNKKLASNASDKAGDAKSDAR
                     KSRKQTGKLDDLNQSIKDLKSKIAGEQYKLSIYSPASSVTTPIAMPRPASSDTTLHP"
BASE COUNT         1328 a         1328 c         1213 g         1250 t
ORIGIN      
        1 ttccggaagt tccgaagcca ttccggaagc ggaagagaag gcacaaccca ccgatggtga
       61 caccgccatt gtccatcaac gcaatcaaca gctggaaaaa gagcttgctt ccctgcgcga
      121 ggacatgcgc aacttcgcgg aagaccagga ggccgttatc gaggagctcc aaagcgcgaa
      181 tgaagagctg ttgtccggca gcgaagaact gcaaagcctc aacgaagagc tggagacctc
      241 caaggaagag atccaaagca caaacgaaga gctgaccacg ctgaaccagg agctcttcga
      301 ccgtaatgaa cagctgaacc tttcccggct ctacgccgaa tcgatcgtcg ccactatccg
      361 tgaacccctg ctcgtgctcg atcacaacat gcaggtgaaa acggccaacc gggcattcta
      421 cgagaaattc ggcgcttcgg aagaagaaac ggaaggcaaa tccctatatg gtcttggaag
      481 gggtgaatgg gatacgccaa cgctccgtgc cgcgctcgga tcgatcttat cccgggaagc
      541 cagtatcaag gacatcgaga tcaactattc tgtcaacgcc ggcggggaaa ggatactttt
      601 gctgaatgcc tcgcgcatct tcaggaagga cagcgccgaa cagctggtat tgctggcgat
      661 ggaagatatt acggaggcca cgaaacgcga gaacgagcag cgtcgttttg ccgacgagct
      721 ggctaaacag gtggacgagc ggacggccag tcttaaagag gcaaacaccg cgcttaaata
      781 ttccaacgaa agcctggagc agttcgcgac gatcgcctcg catgacctgc aggagccgtt
      841 acgcaagatc aggacatttg ctaccattct caataatgag cacagcgcgg acatcgacga
      901 agagaccagg gggctgcttc aaaagatcaa cctgtccgcc gaacggatgg ccgccctgat
      961 ccgggacgtg ctgaattttt cgaaagtatt ggatgccagc gtttttgaag ctaccgatct
     1021 taccctcatc ctgcaaagtg tcatccggga tttcgatctg cagatcgaag agaaacaggc
     1081 ggtaattcgc tacgatccgc tcccctcggt ccaagctgtc cccctccaga tgaaccagct
     1141 tctttacaac ctgctcggca acgcccttaa attctcaaag acaggcctgc caccggtgat
     1201 cgatatctct tcgcgaacgc tgcccccgga cgaattgaaa gagcatcccc agctcgacaa
     1261 ccggctaccg tattgcgaga tcgtctttgc cgatcagggc attggcatag attccaggtt
     1321 cgccgatcgg atcttcctca tcttccagcg gctgaacacc aggcagcact tcgaaggcac
     1381 aggcatcggg ttagccttgt gcaaaaggat cgtcacaaat catggaggag agatctattt
     1441 ccagccaaat gagggcggcg gcgcccgttt ccatgttatt ttaccgctgg tacactaaac
     1501 cggttttccc cgttccagtt tacgcttcag gaacggttcg cggtattgca ataggaaagc
     1561 ctttttggag tcatgatagc tgcgtacggc gccgatcacc ttcaaaaatc taatgtaaca
     1621 ccaggccagg tccagctgat aattcaagaa gccgctgcgg gcgctggacg gaaaaagatg
     1681 atgattgtta tgccactcgc cggcaacgta tcccggccat agctggttga tggaaagatc
     1741 tttcctgttg aagtcgatgc cttccctgtg tttatctttc cccttcccgt gcccttcata
     1801 gttgaaggta cggatgccga tggcccagaa gccggctgcc ccgaataacg cgaataccag
     1861 cgcctccccc cctgcaagat aaaagacaac gccccagaag ctccagttga ggatccatcc
     1921 aaccaaatgc caggccggcc tcaccacgga tccccattgc aggtattgtc gataggtgtt
     1981 tgacttaatg ccgctgccgg ccatcatttt aacgacacgg gtatagtcgt gctccgttaa
     2041 atgttccgag atcgcctggt ggttgacatc ggccaggaaa caatacaaaa aaccggcgct
     2101 tgcattgtaa gggtcccccg gccggtccga cttggcatga tgaacgtggt gcgagatgac
     2161 atacgtttct tccggaacga tattgatcgt cagatttcgc gtcacaaatc gccagaacct
     2221 gttactgaaa gcataggcat ggtgcgtgca ataccggtga taccagatag tgccatgcgt
     2281 acccatgatg atcatgctgt aaataaaagc aacccccaac aggggaaaag agaaatcatg
     2341 aagcaaaaag ataaaaagac aggggatcag cagcagcacc tttgtccaat tgagaaaggc
     2401 cagccaattc tttttggtcc tgaaaatatt caggcgtgcc cagaattcgt tcattatctg
     2461 ccgggtgctc ggaacgacca gacccccttc ggcatccatc catccataag aagggacccg
     2521 caatacgtag tccattaggt ttccttgaaa cataacctgc cgggcataaa atgccccctt
     2581 caaaattaat gcaccccgga taacccgata ttacatcatt cttgtacgaa tgttacataa
     2641 ttcacagatt ttagatccgg gactatcttg caatgaaggt gtgaattata caatattggc
     2701 cgggttttac gtaacaattg ctctccccgg aagccgtaac tttaagctat gcctaaaata
     2761 acattcaata acagcaataa tacattcttt caatccgtca aaaattcggc agatgcttat
     2821 ttcaaaacac aaaaactgaa aagaacgggc aactggaaac tgtatctaaa agcctggata
     2881 ctcatccccg cctccctggg catttactta tttctcctgt cgggtcatta tacctggttg
     2941 atgggtattc tcgcatccgt cttcttgggg ttcacgctgg tatgtatagc cttcaatgtc
     3001 atgcacgacg cgtgccacgg cagcttctct gaaaagaaat gggtaaacaa tatcatggga
     3061 ttgactatga atgcactggg aagtaacgcc tttatttgga agatcaagca caatgtcatc
     3121 catcatacct ataccaacat cgataagatc gacgatgaca ttgccaatgg cccgctattg
     3181 aggcaatgca ccacgcaaaa atggatgccc atccatcgct tccaattcat ctatatgttt
     3241 ttgctttatg gcgtgagtac cctatcctgg gccctgggaa ccgattttgc aaggtacttc
     3301 aggaaaaaga tccatcatac tccgatcaat cggataggcg ttaaacagca cttgctattt
     3361 tgggccagca agctgctgta tgcgttcttt tacgccctgc tgccgatcta cgtattgggt
     3421 tggcagccct ggctgatcgg ttttttggtg gtcaacataa ccatgggcct ttccctatcg
     3481 gtagtatttc aattggcgca tatcgtcgaa aagacgacgt tcgatgtggc cgatggaacg
     3541 cacaaggtca taggttccga atgggccatc catgagatca ggactaccgc cgactttgcc
     3601 cccggtaaca ggatcatttc ctggctggta ggcggcctga atttccagat cgagcatcac
     3661 ctcttcccgc aggtcagcca tatccactat gcagccctga gcaaaatagt gcggcagcaa
     3721 tgcgagctct ttggcctccc ctataactat tatcccactg cgtggcaagc cgtttattcg
     3781 catgtccggc tgatgagaag attgggaacg ggaattcgat aacataaaaa caggtaagcc
     3841 atgaaagatc atgatttggc caatggagag accggcgcca cacccgataa tttaatggat
     3901 gacgaccgca ccgggaccaa ccacggcgaa ttatattcaa attctccttt aaagtacctg
     3961 accgcctcat ctattatcgg ggataaggta cataacgaaa cggacgaaca gatgggagag
     4021 atcatggata tcatgatcga tgtggtcacg ggtaagatcg actattttgt cgtggagttt
     4081 ggcgggttct tcggcattgg cgtaaagtat tttgccatcc cgttccggct gctccgggtt
     4141 gatcccgaaa acaaacgatt catattcaac caggacaaga agacccttga gcaggccccc
     4201 ggttttgata cggatcattg gccccacacc aatattcatc tcgatcaggt ctatagctat
     4261 tggagcttta tgggataatt catccttcgg caccaatccg gccaggacta tcagggatgc
     4321 aaagtcgtat cactgctggc aggcctcggc atcgcaatgg gagtcgttac agatgatgcg
     4381 ggagaatata tgctcaattt gtattgttcg ccggctattt tgcttttcag atcctttata
     4441 ctctgattca ggtcatccaa ttttccggtt tgcttcctcg attttctcgc atcgcttttc
     4501 gcatctcccg ccttatcact ggcattgctg gccagtttct tattagtcgg atcgtcgttt
     4561 aacctttgcg ccgcattggc attatcgctg gccgagttct gtgcattgag ggcggctccc
     4621 tcctggtcct tggttttttg ggcaaccatg ttttgcaact tgccaagttg aagttgatcc
     4681 tggctgatct gtgcgaccaa cttaagtgaa tcgatattcg cctgctgcgc tgtaaccgaa
     4741 gcggagaaaa agagcaagcc ggccggaatt aaataaaata cttttttcat aataactttt
     4801 taattaagac aaaggtgcgt ctttctgcct tcctggttgc tacacgattc aagggaaaga
     4861 ctacatgatt catatcttcc cgattgacgg ttaagggatc ctccgaataa tgcgccagtt
     4921 cgataatgat cgtttttatt ttctctatca atacgctttt cttgtcatcc atcaattcaa
     4981 gcttggactt taaaagggct gtccggaaca attcacgctg gtccgggtaa tgccttcaat
     5041 gatctccgcc tctcccaatt ccacactgct atatctcaaa ccgagttttg tcaactcgtc
     5101 tttgaccatc tttaatgaa
//
LOCUS       DASYSV010000540        10601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1136028, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000540 DASYSV010000000
VERSION     DASYSV010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136028"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VGQ51_16840"
     CDS             complement(<1..703)
                     /locus_tag="VGQ51_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEV9038306.1"
                     /translation="MNELPLDGITVLEFSQYLSGPSAGLRLADLGARVIKIERPGAGD
                     ACRRLSIKNLWVDDSSLLFHTINRNKESFAADIKSPEDRATLIRLIERADVLTHNFRP
                     GVMEAAGLDYAAVCRINPRLIYGAISGYGKEGPWKDLPGQDLLLQSMSGLAYMTGDEQ
                     DNPMPFGLSIADSLCGAQLVQGILAALVRRGKTGAGALVEISMLESLLDFQFEFLTTY
                     HSSGLLPQRSRYSNGH"
     gene            complement(700..1902)
                     /locus_tag="VGQ51_16845"
     CDS             complement(700..1902)
                     /locus_tag="VGQ51_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV9038307.1"
                     /translation="MPETSIQGARPAGTKILKGITWGHSRGFTPLVAAAQRFNEKHPD
                     VSIVWEKRTLQQFADYPIERLIREYDLLIIDHPWVGTAAAQNCVLALDQYLPAAFVEE
                     QASNSVGWSHHSYSYDGHLWALAIDAATPVASYRADLLRHAGVRPPDTWEEVLAIARM
                     KKLAVPGIPIDLLMNFYMFCIAHGARPFVHESHVVDEPVGIAALETMRELYSEIDERF
                     FHANPIAVAECMTNTDDYWYCPFAYGYSNYSREGYASHRLTYGDLVDFKGRGRLRSTI
                     GGTGLAISAQSRYKQEALSFAEWIVSPLIQSTFYVEHGGQPGHRLAWMAGGANRLTNN
                     YFYSVYPTMERSYMRPRYDGYLHFQDHAGEPIREYLLKSRDAASTLQTIDHLYQESRK
                     KHPINLEV"
     gene            complement(1895..2335)
                     /locus_tag="VGQ51_16850"
     CDS             complement(1895..2335)
                     /locus_tag="VGQ51_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEV9038308.1"
                     /translation="MKKFYEEFETGHSRHTNGRTITETDIILHAGQTGDFFPHHMDEE
                     WCKTQPFGRRIAHGTLIFSVAIGLTADSINEVAMTYGYDRLRFIRPVFIGDTIRAEVK
                     IKSKGDHKKPGFGLVVETVEVANQHSELVMVCEHLLLVQKASNA"
     gene            complement(2488..3186)
                     /locus_tag="VGQ51_16855"
     CDS             complement(2488..3186)
                     /locus_tag="VGQ51_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin reductase"
                     /protein_id="HEV9038309.1"
                     /translation="MDHTVKLLATRQVTHDVKEFRVEKPKGYHFVPGQATDVSVNRPE
                     WKDNKHPFTFTGLNDDAWLQFTIKGYPERHGMTELLHQLNPGEEIIIGDVWGAIEYRG
                     PGIFLAGGAGITPFLAIFRQLHKDGQMAGNTLYFSNKTTADIIEREELLKILGVRANF
                     LLTREKQAGDGEHGSPGQRGYFHGRIDKHFLEEQISDFHQPFYICGPDEMVQELSQTL
                     NALGAKPEGLVIEK"
     gene            complement(3197..4273)
                     /locus_tag="VGQ51_16860"
     CDS             complement(3197..4273)
                     /locus_tag="VGQ51_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV9038310.1"
                     /translation="MSLFPVFGRRPGGPRLDRILRSSHYSDSSFQNLVPTEVALKGTS
                     FWKMMREYRNRPADTVPPRPLPSVRTDLKALPDDRPTIVWFGHSSYLLKIGTTHILVD
                     PVFSGHASPVSLFAKAFAGSNIYGVNDMPDSIEAVLLTHDHYDHLDYPTIRRLKEKVT
                     HFYTALGVGSHLESWGVDPARITELDRWEGCGMDAKGLPLAADDPAPAFSLIATPARH
                     FSGRSFKRGGTVWASFVLKGNGISLFLGGDSGYEQHFKDIGERFGPFDLAILECGQYG
                     KNWPYIHMMPEETVQAALDLRAKVLLPVHWGKFTLSLHPWNEPIHRLTAAAEKAGMPL
                     TTPRIGEAVVINGQYPDEPWYDFL"
     gene            complement(4440..5708)
                     /locus_tag="VGQ51_16865"
     CDS             complement(4440..5708)
                     /locus_tag="VGQ51_16865"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV9038311.1"
                     /translation="MRKLPFLLLLFFSCPRFAAAQTISTTAGPDPSVDYARLARIDTF
                     IDGYVRRGWINGVVTIIVHNGRVVQYKGYGYSDVEQHRPMHRDDLFRIASQTKAIVSV
                     GIMQLFEEGKLSLDEPVSDFLPAFKDLRVLDKFNPSDTTYTTVPAKRAITIRDLLTHT
                     AGLDYPLIGTKEMRAIYAKAGTPSGLGVFDADLRERMESLARLPLGHQPGEKWTYSLG
                     VDLLGCLIEVISGKNLEEFLSQRLFGPLGMKDTYFNVPASKAARLVRIYREDSLHRFV
                     PYTSADEGIDPDYPLRHKRYFSGGAGLTSTAWDYAIFLQMLLNGGSYNGVHILSPRTV
                     QLMTSGQLAFNFNGVDNFGLGFDITSAVSAARDVRNEGTFSWGGFYGTTYWADPKARL
                     VCLVMTNQRGMSHYWMEKEVEQMIYASFVR"
     gene            5755..6102
                     /locus_tag="VGQ51_16870"
     CDS             5755..6102
                     /locus_tag="VGQ51_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEV9038312.1"
                     /translation="MAKTTTDRPPVVHHVFFWLKNSSSTEDRDLLVAGLKTLSNIPAI
                     KELYIGVPADTEQRGVVDASWQVSELMFFEDLDAQAGYQEHPLHMAFVKKCSHLWEKV
                     IVYDASNIYSVVS"
     gene            complement(6167..6601)
                     /locus_tag="VGQ51_16875"
     CDS             complement(6167..6601)
                     /locus_tag="VGQ51_16875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEV9038313.1"
                     /translation="MGIADRLFNMNKDKAAANLKAGQDFLEENKKTAGIVALPSGLQY
                     MILTEGSGPKPAASNKVTCHYHGTLIDGTVFDSSVRRGQPATFPLNAVIKGWTEGLQL
                     MPTGSKWRFFIPPHLGYGDRQVSSQIGPNSTLIFDVELISFS"
     gene            6845..8074
                     /locus_tag="VGQ51_16880"
     CDS             6845..8074
                     /locus_tag="VGQ51_16880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEV9038314.1"
                     /translation="MNQPAALSRRTYILYVVILGLLTTIGPFSIDMYLPGFDDIARSL
                     GTTPARVALSLSSYFIGISLGQLIYGPLLDRYGRKRPLYAGLAVYVVATFVCMQAKDI
                     DTLIALRFIQAVGSCAAQVAAMAMVRDLFGAKESARVFSLLLLVIGLSPMIAPTVGSY
                     VVVSYGWRTVFLILAILTMIITALTVFFLPESYSPDKDFSLKPVAIIKNFLTVLRIRQ
                     FMIYVLVESFAFAGLFAYVSGSPILFMNIFHVDKRTYGWIFAFLSVAFIGLSQFNVIL
                     LKRFSGEKIIRVALTGQVIVSLTFMAGAAAGWYGLGLTIFFLFLFLAFMGFTYPNAAA
                     LALAPFDKNAGTASSLLGMLQLGVGALASIAVSAFMKGTSFPMAAIIAVSSVIALLIL
                     LYGSLATRLSGSNRSIR"
     gene            complement(8023..8742)
                     /locus_tag="VGQ51_16885"
     CDS             complement(8023..8742)
                     /locus_tag="VGQ51_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV9038315.1"
                     /translation="MKTVLILGANSDVAKEAMALYVQKGFRVIAASRNLQALRDFIAA
                     RGLPAQQVTLCPFDATAFDIHPAFYAALPFKPHIVLYAAGFQVTNEEALVDWEGTRRM
                     METHYAGAVSILNLVVMDKDNDHLERIVGLSSLSGVRGRKSNFVYGSTKAAFTTYLAG
                     LRQYLSPRHVIVNAVVAGYIRSKMTVHLDLPASLLMEPAFIARHIVSPPKKFAIVPGW
                     KWKIIYRILRLLPESLVARLP"
     gene            complement(8739..>10601)
                     /locus_tag="VGQ51_16890"
     CDS             complement(8739..>10601)
                     /locus_tag="VGQ51_16890"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEV9038316.1"
                     /translation="HNSYFSCMNYQRVTPAYALLLAVLFCTPAFATQVLPDTTIIQNS
                     VARREIDLDGRWNYIIDPYQTGYYDYRRKPYDESPSGKGGFYDDKKPADKGELVEYDF
                     DHSPTLKVPADWNSQDDKLQFYEGAIWYRRKFTAAPRAGRRYFLCFGAANYESHVYLN
                     GKKLGMHKGGFTPFQFEVTDRLATGENTVVVMVDNSRHKDEIPTVSTDWWNYGGITRD
                     VWIASAPSTFIRDYQLQLKKDEPGVISGYVQVSGASGSLAVSIDIPEAGVHGMVTTDG
                     SGRALVSIPVKKLEYWSPEDPKLYTVTLKTADDRIEDKIGFRTISVRGKDILLNGKPV
                     FLRGISIHDEDPLIPGRNRGEGDLRMLLTWAKQLHCNYVRLAHYPHNEIMLRLADELG
                     LMVWAEVPVYWTISWEDPDTYANAQKQLTDLIDRDRNRASVIIWSIGNETPLSEPRLS
                     FMSRLADRAHALDNTRLVSAALEVHRHGNEVIVDDPLGEKLDLVSFNEYAGWYWGDKP
                     ADLANYHFTIKYDRPVIITEFGGDALGGYHADSLTRWSEEYQASLYRAQLQMLRRIPA
                     LRGMTPWILCDFRSPRRQNPVYQDFWNRKGLISGTGQKKEAFFVLKEFYEEVGK"
BASE COUNT         2334 a         2868 c         2914 g         2485 t
ORIGIN      
        1 gatgcccatt gctatatctg cttctctgcg gcaatagtcc gctgctatga taggtggtca
       61 ggaattcgaa ctgaaaatcc agcagtgact cgagcatgct gatctcgaca agcgcccctg
      121 ctcccgtctt cccgcgtcgt accagcgccg cgagaatgcc ctgcaccagc tgagcgccgc
      181 aaagactatc ggcgatcgac aatccgaacg gcatgggatt gtcctgctca tcgcccgtca
      241 tataggccag cccgctcatg gactggagca gcagatcctg tccgggtaga tccttccagg
      301 gaccttcctt gccatatccg ctgatagcgc catagatgag acgggggttg atccggcaga
      361 cggcggcata gtccagaccg gccgcttcca tcacgccggg acggaaattg tgcgtcagta
      421 catccgcgcg ttcgatgaga cgaataagag tggccctgtc ttccggagat ttaatatcgg
      481 ccgcaaagct ttccttattc ctgttgatcg tatgaaaaag cagggaggag tcgtcgaccc
      541 agagattttt gatcgaaagc ctgcggcagg cgtcgcccgc gccgggacgt tcgatcttga
      601 tcaccctggc tcccagatca gccagccgca atccggcgga aggtcccgat aaatattggc
      661 tgaactccaa caccgttatt ccgtctaaag gcaattcatt catacttcaa ggttgatggg
      721 gtgtttcttc cggctttcct ggtacaggtg gtcgatggtt tgaagggtcg atgcggcgtc
      781 gcgacttttc aataaatatt cccggatagg ttctcccgca tgatcctgga aatgcaggta
      841 tccgtcataa cgcggacgca tatagcttct ttccatggtc ggatagacgg aatagaaata
      901 gttgttcgtg agccggttgg cccccccggc catccaggcc agccggtgcc cgggctggcc
      961 cccgtgttct acatagaagg tcgactgtat cagcggtgag acgatccact ctgcaaaaga
     1021 aagcgcttcc tgtttataac ggctctgcgc cgaaatggcc agccccgtcc cgccaatcgt
     1081 gctacgcaac cgtccacggc ctttgaaatc gaccagatcg ccataggtca gccggtgact
     1141 ggcatatcct tcccgggaat agttggaata accgtaggcg aacggacaat accagtagtc
     1201 atcggtgttg gtcatgcact ccgccacggc gatcggattg gcgtggaaga atctttcatc
     1261 tatttcggaa tacagttcgc gcatcgtctc cagcgccgca attccgacgg gctcgtccac
     1321 gacatgcgat tcgtgtacga aaggccgggc gccatgggcg atacagaaca tatagaaatt
     1381 catgagcaga tcgatcggga tcccgggtac cgccagcttt ttcatccttg cgattgccag
     1441 gacttcttcc caggtgtccg ggggacgcac accggcatgg cgtaacaggt ccgcgcgata
     1501 gctggcgacg ggtgtcgcgg catcgatggc caatgcccag aggtgaccgt cataagaata
     1561 actgtgatgg gaccatccga cgctgttcga agcttgttct tcgacgaatg cggcgggaag
     1621 atattgatcc agcgcaagaa cgcaattttg cgcggcggcc gtacccaccc aggggtgatc
     1681 gatgatcagg agatcgtatt ccctgataag ccgttcgata ggataatccg caaactgttg
     1741 caaggtcctt ttctcccaga cgatactgac gtccgggtgt ttttcgttga agcgctgtgc
     1801 cgccgcgacc agtggtgtga agccgcggct atggccccag gtgatacctt tcaggatctt
     1861 cgtacccgcc ggccttgccc cttgtatgga tgtttcaggc attggatgct ttttgaacca
     1921 gtagcagatg ctcacagacc atgaccagct cactatgctg gttggcgact tcaaccgtct
     1981 cgacaacgag accgaaaccg ggttttttat gatccccctt actcttgatc tttacttctg
     2041 cacggatggt atcgccgata aagacggggc gaatgaaccg aaggcggtcg tatccatagg
     2101 tcatggcaac ctcgttgata ctgtctgccg tcagccctat agcgacgctg aatatgaggg
     2161 ttccatgggc aatacgccgg ccgaaaggct gggtcttgca ccattcttcg tccatatgat
     2221 gggggaaaaa atcacctgtt tgaccggcgt ggaggatgat gtcggtctcc gtgatcgtac
     2281 ggccgttggt gtggcggcta tgcccggtct cgaactcttc gtaaaatttc ttcatgttca
     2341 gcaatcgtgg gtctctccgg cttgggatcc gtcctgttct tcaggctctc ctctattttt
     2401 ccggcgaagg gatgattgag aaatgaaggt accgacgggg ggtagcgccg gcaatgtcag
     2461 gtttgactat tttgatggac tattttacta tttttcgatg accagtccct caggtttggc
     2521 gcccagcgca ttgagcgtct gcgacagttc ctggaccatt tcatcgggtc cgcagatata
     2581 gaaaggctgg tggaaatcgc tgatctgttc ctcgaggaag tgtttgtcga tccggccatg
     2641 gaaatagccg cgttggcccg gggatccgtg ctccccgtcg ccagcttgct tttcgcgtgt
     2701 cagcaggaag ttcgcgcgta cgccgaggat cttcagcaat tcctcgcgct cgatgatgtc
     2761 tgccgtggtc ttgttggaga agtagagggt attgccggcc atctgcccgt ctttatgcaa
     2821 ttgccggaag attgccagga atggcgtgat ccccgcaccg ccggcgagga atatgccggg
     2881 gccgcgatat tcgatggcgc cccagacatc gccgatgatg atctcctcgc cgggattgag
     2941 ctggtggagc aattcggtca tcccatgtct ttcaggataa cctttgatcg taaactggag
     3001 ccaggcgtcg tcattcagac ccgtaaaggt gaacgggtgt ttattgtctt tccattccgg
     3061 gcggttgacg gatacgtcgg tagcctgacc ggggacgaaa tgatagcctt tgggcttttc
     3121 cacgcgaaat tctttgacgt cgtgggtgac ctgtctggtc gcgagtaatt tgacggtgtg
     3181 atccatagat tgcaatttac aaaaaatcgt accagggctc gtcgggatat tggccgttga
     3241 tgacgaccgc ctctccgatc cgcggggtgg tcagcggcat tccggccttt tcagcggcag
     3301 ctgtcaggcg gtgtatgggt tcattccagg ggtggaggga cagcgtgaat tttccccagt
     3361 ggacaggtaa taagactttt gcgcgaagat ccagcgctgc ctgaaccgtc tcctccggca
     3421 tcatatgaat ataaggccag ttcttgccat attgtccgca ttcgaggatg gccagatcga
     3481 aggggccgaa ccgctcgccg atgtccttaa aatgttgttc ataaccggag tcgccgccga
     3541 ggaaaaggct gatgccgttg cctttcagga caaatgacgc ccagaccgtg cctcctcttt
     3601 taaagctgcg cccggaaaaa tgacgcgcgg gtgtggcgat gagtgagaac gccggcgccg
     3661 ggtcatcggc agccaagggt agtcctttcg catccatgcc acatccttcc caccggtcca
     3721 gttccgtgat gcgggccgga tcgacgcccc aggattcaag gtgtgagcct acgcccaatg
     3781 cggtataaaa atgtgttact ttctccttca gccgccggat ggtagggtaa tccagatgat
     3841 cataatgatc gtgcgtcagc agcacggctt cgatgctatc cggcatatcg tttactccat
     3901 atatgttgct gccggcgaag gcctttgcaa ataaggatac aggcgaagca tgcccgctaa
     3961 aaacagggtc gacaaggatg tgcgtggttc cgatctttag cagataggac gaatgcccga
     4021 accaaacgat ggtcggccga tcatccggca gagccttcag atccgtccgc accgaaggca
     4081 gcggccgcgg cggaacggtg tcagccgggc ggttccggta ttcccgcatc attttccaaa
     4141 aggaagtgcc tttcaaagcc acctcggtag gaacgaggtt ttgaaagctg ctatcgctgt
     4201 aatgcgaaga tcgtaaaatg cggtccagcc ggggtccgcc gggcctcctg ccaaaaacgg
     4261 ggaataggct catggcctcc caaacaaaca ggtcgggata tggttgggcg gatattagat
     4321 tttccctcgc gtgttccgca agggccaaag ccttgttgaa aaaagtgggg ttacccaaac
     4381 aattctccaa agaagctcac tcctgattca tcgactattc ctattagcgg atatttaccc
     4441 taccgcacaa aactcgcata gatcatctgc tccacctcct tctccatcca ataatggctc
     4501 atcccccgct gattcgtcat gacgagacaa acaagccgcg ccttcgggtc cgcccaatag
     4561 gtggtgccat aaaacccacc ccagctgaat gtaccttcat tacgaacgtc gcgggcggct
     4621 gagaccgcgc tggtgatgtc gaatcccaat ccgaaattgt cgaccccatt gaagttgaaa
     4681 gcgagctgac cgctggtcat cagctgcacg gtacggggtg agaggatatg cactccgtta
     4741 tagctgccgc cattcagcaa catctgtaaa aagatcgcat aatcccaggc agtcgacgtc
     4801 agccccgctc caccggaaaa ataacgctta tgccgcaggg gataatcggg gtcgatgcct
     4861 tcatctgcgg atgtatacgg aacgaatcga tgcaaagagt cttcgcggta tatgcgcacc
     4921 agcctggcgg cttttgaggc gggtacgttg aaataggtgt ccttcatacc cagcggcccg
     4981 aaaagccgct gtgataaaaa ttcctcgaga tttttaccgc tgatcacttc tatcaggcat
     5041 ccgagcaggt ccacgcccag actataggtc catttttcgc cgggctggtg gcccagcgga
     5101 agacgggcaa gcgattccat cctttcgcga aggtcagcgt cgaatactcc taaacccgac
     5161 ggggtgcctg ctttggcata gatggcacgc atttccttcg tgccgatgag tgggtagtcg
     5221 agaccggcgg tatgcgtcag cagatcgcga atggtaatgg cgcgtttcgc gggtacggta
     5281 gtataggtcg tatccgaagg attgaatttg tccaaaaccc gcaggtcctt aaatgccggc
     5341 agaaaatcgg ataccggctc atcgagcgag agcttccctt cttcgaagag ctgcatgatc
     5401 ccgacactga cgatggcctt ggtctgagag gcgatgcgga aaaggtcatc ccggtgcatg
     5461 ggccggtgct gttcgacatc ggaatagccg tatcctttat attggacgac ccggccgtta
     5521 tgcacgatga tcgtgactac gccattgatc catccccggc gcacatagcc gtcgataaaa
     5581 gtatcgatcc gggctagtct cgcgtagtcg accgaaggat cgggccccgc cgtagttgaa
     5641 atggtttggg cagcggcaaa acgaggacat gaaaagaaca gcagcaataa aaaaggtagt
     5701 tttctcatat ggggcaatat ttcatttatg actatcttcg aacaaatcgc aatcatggca
     5761 aagaccacca cagacaggcc accggtagtg catcacgtat ttttctggtt gaaaaactct
     5821 tcctccaccg aagataggga tttgttagta gccggtctca agaccctatc gaacatcccc
     5881 gccattaaag aactgtatat cggcgtaccc gcggataccg aacaacgcgg ggtggtcgat
     5941 gcctcctggc aggtatcgga actgatgttc ttcgaagacc tcgacgcgca ggccggctac
     6001 caggaacatc cgctgcacat ggcattcgta aagaaatgca gccatctttg ggagaaagtg
     6061 atcgtatacg atgccagcaa tatatacagt gtggttagct gaagcagtca cgcccattcg
     6121 gatcggtcgc agtcacaccc attcggatct gtcgctttca cgcggatcag ctgaatgaga
     6181 tcagctccac atcgaagatc agggtgctgt tcggtccgat ctgcgaactg acctgccggt
     6241 ctccatatcc gagatgcgga ggaatgaaaa aacgccattt actacccgta ggcatcaatt
     6301 gtagtccttc cgtccaacct ttaatgacgg cattcagcgg gaaggttgcc ggctgaccgc
     6361 ggcggacact gctgtcgaag accgttccgt cgatcagagt cccgtgatag tgacaggtga
     6421 ccttattgct ggcagcaggt ttcggcccgc tgccttcggt gaggatcata tattgtaatc
     6481 cgcttggcaa cgcaactata cccgcagtct ttttgttttc ctcgagaaaa tcctggccgg
     6541 ccttgagatt ggcagcagcc ttgtctttgt tcatattgaa taaccgatcc gcgattccca
     6601 tatctgtttt ttgcaaattt actgaattgg ctcgtcacca cccttacgct cgtataatat
     6661 agtcagattg gtctcgagcg gaggttggac gattactgaa tcccaatgta cccgccggcg
     6721 gccggcgaaa catctcttct attctgcgaa aaagacccat tgcacaaaga tacaaaatcg
     6781 cgacccggcg ccggcgtatc gtacatcttc ctattttagc ggaaagtttg aaaaaacgga
     6841 gcagatgaat caaccggcag cgctatcccg acgaacctat atcctctatg tcgtaatcct
     6901 cggattactg acgacgatcg gaccattttc aatcgatatg tacctgcccg gttttgacga
     6961 catcgcccga agtctgggga cgactccggc cagggtggcg ctctcgcttt ccagttattt
     7021 tatcggaatc tccctgggac agctgatata tggcccgctg ctggaccgct atggccgcaa
     7081 acggccctta tatgccgggt tggccgtgta tgtcgtcgcg acgttcgttt gtatgcaggc
     7141 aaaagatatc gacacgttga tcgcgttacg tttcatccag gcagtgggca gttgcgccgc
     7201 gcaggtagcc gccatggcga tggtccggga ccttttcggc gcaaaagaaa gcgccagggt
     7261 cttctcgctg ttgttgctgg tgatcggcct gagcccgatg atcgccccga cggtgggtag
     7321 ttatgtagtc gtcagctatg gatggaggac ggtcttcctg atcctggcga tcctcaccat
     7381 gatcatcact gcgctgacgg tcttctttct gcccgaaagc tattcccccg acaaggactt
     7441 ctcgctgaaa ccagtagcca tcataaaaaa tttcctgacc gtactccgta taagacaatt
     7501 catgatctat gtgctggtgg aatcctttgc tttcgcgggg ctctttgcct atgtgtccgg
     7561 ctcccctatc ctcttcatga atatctttca tgtcgacaaa cgcacctatg gatggatctt
     7621 cgcgttcctg tcggtggcgt tcatcggact gagccagttc aacgttattc tgctgaaaag
     7681 attctccggc gaaaagatca tccgggtggc gctgacagga caggtcattg tttcactgac
     7741 attcatggcc ggcgccgcgg caggatggta tgggctgggg ctcaccatct tctttctttt
     7801 tctattcctg gcttttatgg gtttcaccta tccgaatgcg gcggcattgg cgctggctcc
     7861 attcgacaag aatgcaggga ccgcctcttc tctgctggga atgctccagt tgggcgtcgg
     7921 cgccctggcc tccattgcgg tcagcgcttt tatgaaaggg acgtcgtttc cgatggcagc
     7981 gatcatcgcg gttagctcgg tgattgcact cctgatcctg ctctatggca gcctggccac
     8041 caggctttcc ggcagtaacc ggagtatccg gtagatgatc ttccacttcc aaccgggaac
     8101 gattgcgaac tttttggggg gcgagacgat gtgccgggcg atgaaagcgg gctccataag
     8161 caacgaagcc ggaagatcga gatgcacggt catcttgctg cggatataac ccgcaactac
     8221 cgcgttgacg atgacatgac gaggggaaag atactgccgc aatccggcga gataggtggt
     8281 aaacgccgcc ttggtgctgc catagacgaa attgctctta cggccccgga cgcccgataa
     8341 ggaagacaga ccgacgattc gttccaggtg gtcgttatcc ttgtccatca ccacaagatt
     8401 aagaatggag acggctccgg cataatgtgt ctccatcatc cggcgggtgc cttcccagtc
     8461 aacaagggct tcttcattcg tcacctggaa ccctgctgca tataatacga tgtggggttt
     8521 aaagggcagg gcggcataaa aagcgggatg tatatcgaat gccgttgcgt cgaaggggca
     8581 aagggtcact tgttgggcgg gcagcccgcg ggccgcaatg aaatcccgca gcgcctgcaa
     8641 attgcgcgag gccgcgatca cccgaaagcc tttctgtaca tatagggcca tcgcttcttt
     8701 cgccacgtca gagttggcgc cgaggatgag aacggtcttc atttccctac ctcctcataa
     8761 aattctttca gcacaaaaaa ggcctctttc ttttgtcccg tcccggagat cagccctttc
     8821 ctgttccaga agtcctggta tacgggattt tgccgccggg gagaccgaaa atcacagagt
     8881 atccagggcg tcatcccccg caacgcaggg atacgccgga gcatctgcag ctgagcgcga
     8941 tacaaagacg cctggtattc ctcgctccac cgggtaagtg agtcggcatg ataacctccg
     9001 agcgcatcgc cgccaaactc tgtaataatg accgggcggt catacttgat cgtgaaatga
     9061 tagttggcca gatcggcagg cttgtcaccc caataccaac ctgcatattc gttgaagctg
     9121 accaggtcca gcttttcgcc caacggatcg tcgacgatca cctcattgcc atgacgatgc
     9181 acttccagtg ccgccgatac caggcgggta ttgtcaaggg catgtgcgcg gtccgcaagc
     9241 cggctcataa aggaaagacg gggctcgctg agcggcgtct cattaccgat agaccaaatg
     9301 ataacggatg ccctgttgcg gtcccgatcg atgaggtccg tcagctgttt ttgcgcgttg
     9361 gcatacgtgt cgggatcctc ccaggagatg gtccaataga ccggtacttc ggcccagacc
     9421 attagcccga gctcatcggc caatctcagc atgatctcgt tgtgaggata gtgtgcgagg
     9481 cggacataat tgcaatgcag ctgtttggcc caggtgagca gcatccgcaa gtcgccttca
     9541 ccgcgattgc ggccggggat caacgggtcc tcgtcatgga tcgaaatgcc ccgaagaaaa
     9601 acgggtttgc cgttgagcag gatgtccttt cccctcactg agatggttcg aaagccgatc
     9661 ttatcctcta tgcgatcgtc tgctgttttt agcgttacgg tatagagctt cggatcctcg
     9721 ggcgaccagt attcgagctt ttttacaggg atggatacaa gagcccggcc agatccatcc
     9781 gtcgtcacca taccatgaac ccctgcttcg ggaatatcga tgctaacggc cagcgaaccg
     9841 gaggcgcccg atacctgtac atatccggag atcacgcccg gttcgtcttt cttcaattgc
     9901 agctggtaat cgcgaataaa tgtcgaaggc gccgatgcga tccatacatc gcgggtgatg
     9961 ccgccatagt tccaccagtc ggtgctcacc gtaggtatct cgtctttatg ccgggaatta
    10021 tccaccatga cgacgacggt attctcgccc gtggcgagcc ggtcggtcac ttcgaactga
    10081 aaaggggtga atccgccttt gtgcatgccc agctttttcc cgttgagata gacatgagac
    10141 tcatagttgg cagcgccgaa acaaaggaaa tatcgacggc cggcccgcgg agccgcggta
    10201 aactttcgtc ggtaccagat ggcgccttca tagaactgca gcttgtcgtc ctgtgagttc
    10261 cagtcggccg gaaccttgag cgtcggcgaa tggtcgaaat catattcgac gagctcgccc
    10321 ttgtcggcgg gctttttgtc atcgtagaaa cctcccttgc ctgaagggga ttcatcgtaa
    10381 ggcttgcgcc ggtaatcgta gtagcccgtc tggtaagggt ctataatata gttccagcga
    10441 ccgtcgagat cgatctcgcg gcgcgcgacg ctgttttgga tgatcgtggt atcgggcagg
    10501 acctgcgtgg cgaaagccgg tgtgcaaaag agaacggcaa gcagcaaggc atacgccggg
    10561 gtaacgcgtt gatagttcat gcagctaaaa tacgaattat g
//
LOCUS       DASYSV010000541         3607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1518042, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000541 DASYSV010000000
VERSION     DASYSV010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518042"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..2004
                     /locus_tag="VGQ51_16895"
     CDS             73..2004
                     /locus_tag="VGQ51_16895"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEV9038317.1"
                     /translation="MRKPALFILFLFYFVSARAQLIELAAGGVRITDSVACYMTTTDL
                     STLPDTTTSVSRARFLQLLNDNPRRHLFWMIVGIRNDNDSTLDCSLDYGELDDIEVWY
                     LPDCGLTWAKRNGIVFQTAGGTLRPSPEDASYIQRQSHLLPLRLPPHCPTRLVVRIRQ
                     RTDEYSFEGLTLYDARSLNASLIGNLARDRSFLIIQMLFQGFLICQLIYALFQWLIIR
                     RPEYGYYLLYMVLISLYFLSKQENLFGVDFLFSRWPLLKIWFGKTLLILPYYIYFRFI
                     RSFLDIPRNYPALNRWIVPLEYFLLAYAVADLAFMFITFDRKTQTIVYTTVFAAIFLL
                     TMGFMIYLYRHRQTLIYYIISGSLLVATGHILGLIFSYLEIDRHMDLGVPDVFVFAQA
                     GIVLEVLCFTAGLSYKNRKTEQEKIAGQEELIEQLKANELLQVRMQHIRNKIAQDLHD
                     DIGSTLSSISILSDLAIRGNNNSKLLKTMNEIKDSSMMLMERMDDIVWSFNPRNDSLD
                     NLLMRIRHFATTLFEARGINYTIDIQQNISEVHLPMEYRQHIYLILKEAINNLVKYAN
                     ASEATIEVRFDHRDLTLSVRDNGRGFETASATRGNGLAGMRHRAALMNARVSITSSPG
                     AGTSISLEVELSPPTQKIL"
     gene            2086..2715
                     /locus_tag="VGQ51_16900"
     CDS             2086..2715
                     /locus_tag="VGQ51_16900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9038318.1"
                     /translation="MIKIAIVEDNNTLRSSLESLFNRTEGMRCVASLNNLLNVVSEIG
                     SALPDVILMDIGLPNISGIEGVRTVKTHFSAIQILMFTVFDDDEKIFDAIRAGASGYL
                     LKKTPPSEIIEAIRELYQGGAPMTSSIARRVIRSFQASPPSIIEDFQLTVRETEILYS
                     LVDGLSYKKIADKYCVSISTIRTHICNIYHKLHVNSKAEAVARVLGPRK"
     gene            2886..>3607
                     /locus_tag="VGQ51_16905"
     CDS             2886..>3607
                     /locus_tag="VGQ51_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038319.1"
                     /translation="MKKLLTVAALPFLLAALSIPSVQAQVTNPGTTAGQGATDHVNNN
                     VSNGINNGLDKTEGAIKGLFRKKSKTPKTDTTKTAAATTNSAGANATASTNPGGPMNL
                     TAYSNYDFVPGEQIVFEDHFTDDHDGEFPAHWKLTAGQAVINNVGSDPAFFLTEGNYV
                     RVAPRMKTEKDYLPDNFTIEFDFYPTDGSSSPILLFTTNTDDGRHIQFGTDVSTGYFP
                     NDLSNPYPGDKENFNNKWHHAA"
BASE COUNT          834 a         1280 c          759 g          734 t
ORIGIN      
        1 aggcgcatct tgacatcgat accgcccccg gccagggtac cactgtcacc ctcacgatta
       61 aaatctcgtg acgtgcgcaa accggctctc tttatactat tcctttttta ctttgtttcc
      121 gcccgggccc aactgatcga actagccgcc gggggcgtcc gcatcaccga ttccgtcgcc
      181 tgttacatga caaccaccga cctgtccacc ttacccgata ctaccacctc cgtctcccgc
      241 gcgcgtttcc tgcaactgtt gaacgacaac ccccgccggc acttgttctg gatgatcgtt
      301 ggtattcgca atgacaacga cagcaccctc gactgctcgc tcgactatgg cgagctggac
      361 gacatcgaag tctggtatct ccccgattgc ggtctgacct gggcaaaacg caacggtatc
      421 gtcttccaaa ccgcgggcgg tacccttcgt ccctcacccg aggacgcctc gtatatccag
      481 cgtcagtccc atctgctgcc ccttcgcctg cccccacatt gtcccacccg gctggtggtc
      541 cgcatccgcc aacgcaccga cgaatattca ttcgaaggtc tcaccctcta tgacgcccgt
      601 tcgctcaacg ccagcctcat cggcaatctc gcccgggata ggtcctttct catcatccag
      661 atgctattcc agggtttcct gatctgccag ctcatatacg cgctgttcca atggctgatc
      721 atccgccgcc cggagtacgg ctactatctt ttgtacatgg tcctcatctc tctctatttc
      781 ctgagcaaac aggaaaacct gttcggcgtc gatttcctgt tttcgcgctg gcccctgctc
      841 aagatctggt tcgggaagac cctgctcatc ctaccctatt acatctactt cagatttata
      901 cgcagcttcc tcgacattcc gcgtaactat cccgcactca atcgctggat cgtcccgctc
      961 gaatatttcc tgctcgccta tgccgttgcc gatctggctt tcatgttcat caccttcgac
     1021 cgtaaaaccc agacgatcgt ctataccacc gtcttcgccg caatattcct gctgaccatg
     1081 ggtttcatga tctatctcta ccgccaccgg cagacgctga tctattacat catctccggc
     1141 agcctcctgg tcgccaccgg ccacatcctc ggactcatct tcagctatct cgagatcgac
     1201 cgccatatgg atctcggcgt ccccgatgta ttcgtttttg cacaggccgg tatcgtcctc
     1261 gaggtgctgt gcttcaccgc gggtctttcc tataagaacc ggaagaccga acaggaaaag
     1321 atcgccggcc aggaagaact gatcgaacag ctgaaggcga acgagctgct ccaggtccgg
     1381 atgcagcata tccgtaacaa gatcgcccag gacctccacg acgacatcgg atctaccctc
     1441 agctcgatct ctatcctcag cgacctggcc atccgcggca acaacaactc caaattgctg
     1501 aagaccatga atgagatcaa ggatagctcc atgatgctca tggaacgcat ggatgatatc
     1561 gtctggagct tcaacccccg caacgattcg ctggacaacc tcctgatgcg tatccgccat
     1621 ttcgccacca cgctcttcga agcccgcggc atcaactaca ccatcgacat ccagcaaaat
     1681 atcagcgagg tccacctgcc gatggaatac cgccagcaca tctatctcat cctcaaagag
     1741 gcgattaaca atctcgttaa atacgccaac gcctccgaag ccacgatcga agtgcgtttc
     1801 gatcaccgcg acctcaccct ctctgtccgc gacaatggcc gcggtttcga gaccgcctcc
     1861 gccacccggg gcaacggcct cgccggcatg cgccaccggg ccgccctcat gaatgcccgc
     1921 gtctccatca ccagcagccc cggcgcaggc acctcgatca gcctggaagt agagcttagc
     1981 ccccccaccc aaaaaatcct ataaacatcc gattggaatc tccagaaatc ttccccatat
     2041 ttggccgccg ggcggccgcc cacaacgcac accgctcatg aaaccatgat caaaatagcc
     2101 atcgtcgagg ataacaatac ccttcgttcg agtctggaaa gccttttcaa ccggaccgaa
     2161 ggcatgcgtt gcgtggcatc cctgaacaat ctgctcaatg tcgtcagcga gatcggctcg
     2221 gcgctgccag atgtcatcct catggatatt ggacttccca atatatcggg catcgagggg
     2281 gtgcggacgg taaagaccca cttctccgcc atccagatcc tcatgttcac cgtcttcgac
     2341 gacgatgaaa agatcttcga cgccatccgc gcaggcgcct caggttatct tcttaaaaag
     2401 acacccccct ccgaaattat cgaagccatc cgcgagctgt accagggagg cgcccccatg
     2461 acctccagca tcgcacgccg ggtcatccga tccttccagg cttctcctcc aagcatcata
     2521 gaagatttcc agctcaccgt ccgcgagacc gagatactct actcattggt cgacggtctc
     2581 agctataaaa agattgccga caaatactgc gtcagcatca gcaccatccg cacccatatc
     2641 tgtaacatct atcacaagct ccacgtcaat tccaaggcag aagcggtagc ccgcgtcctc
     2701 ggcccgcgaa aataacgacc ccccgcgcga gccgcgaccc cacggacgag ccgcatcccc
     2761 cgcacaagcc gcccacccac accgaccacc ccgccgcgcg cacatgccgc atccccccaa
     2821 aatcatatat tcatccgatt gacccgcgcc ggcaattgtc gtctttttgc taaaacaaca
     2881 cgaccatgaa aaaattgctc accgtagccg ccctgccctt cctgctcgcg gcactgtcga
     2941 tcccctccgt ccaggcccag gtcaccaatc ccggcaccac agccggacaa ggcgcaaccg
     3001 accacgtcaa taacaatgtc tccaacggca tcaataacgg cctcgacaag accgaaggcg
     3061 ccatcaaagg cctcttccgg aaaaagagca aaacaccaaa gactgacact actaaaacgg
     3121 cagccgccac gaccaactcc gccggcgcca atgcgaccgc atccaccaac cccggcggcc
     3181 ccatgaacct caccgcctat tccaactacg atttcgtacc cggcgaacag attgtcttcg
     3241 aagaccattt caccgacgac cacgacggcg aattccccgc tcactggaaa ctcaccgcgg
     3301 gacaggccgt catcaataat gtcggttccg acccggcctt cttcctcacc gaaggcaact
     3361 acgtccgcgt ggccccccgt atgaagacgg aaaaagacta tctccccgac aatttcacga
     3421 tcgaattcga tttctatcct accgacggct ccagctcccc gatcctgcta ttcacgacca
     3481 acaccgacga cggccgccac atccaattcg gcaccgacgt atccaccggc tatttcccta
     3541 acgatctcag taacccctac ccgggcgaca aagaaaattt caataacaaa tggcaccacg
     3601 ccgctat
//
LOCUS       DASYSV010000542         4581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1136226, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000542 DASYSV010000000
VERSION     DASYSV010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136226"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3155)
                     /locus_tag="VGQ51_16910"
     CDS             complement(<1..3155)
                     /locus_tag="VGQ51_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEV9038320.1"
                     /translation="MKKIYTIVILLSALRLAAQPYNNEWIDYSKTYYKFKVGATGLYR
                     IPQSVLAAAGMGAVPVQNFQLFRNGQEVPVYTSVSSGVLGASDFIEFWGVMNDGAPDA
                     PLYRSPSYQHTTHWSLETDTAVYFLTVNPTGSTFHFSDVANNVAGSPLTPEPFFMYST
                     GSYFKNGGVNPGFAQVVGEYIYSSSYDIGEFWASPAIPPGTPLTDTRNNLFVYPGGPN
                     ASIKFGMVGAADNARTVQLTVNGSVVEDTTMNSFYDVQATKPVPLSLISGNTATINYI
                     NNSTVTTDRMVASFYELSYPRQFNFGGQDNFAFQLPARTAGYLLNIANFGIVAGATPV
                     LYDLTNGLRYTGVVSGTTVSFALNGSTATYNLVLVNEDPSTVHAVSTLTQKTFQNFAN
                     SSVQGNYLIITNPLLYNDGTNDGINPVNDYKTYRSSPAGGSWDVQIYDINELVDQFGF
                     GIKKHPDAVQNFIRWARAKFTVKPQFIFIIGHGMCYNDYNAASEVNHDPNADLLDLVP
                     TFGYPASDNKLSANDGAGATPLTPIGRLSVVSGAEIETYLTKVKEYENLQVSAPNTID
                     GRLWMKNGVHLTGVSEPYLGTILCNYMNYYGSLISDTLMGATISTFCDGNATAVTQIP
                     STLLADLFSTGITELCYFGHSANDVLAYNLNEPTDYNNTGGKYPVFYINGCDAGDFFV
                     YDVSRLTSSKTLSEDWLLSKERGAIAFVASTHLGIVNYLNIMLSGLFQDMTTIDYGKP
                     IGIIEKDALNYLTTAAPGDFFARLHAEEMTMHGDPVLKLNQGPTDYDIEASQVVINPS
                     FVSISNSTFEVKARFYNLGKAVSDSVGVLVTRTYPNGNIITLLSKKIRGIRYSDSIDI
                     SVPIVGIRDKGDNKITVTIDPGNVIPEATYANNTVTTDVFIYQDEANPVYPYNYAIIN
                     TPTSKLYASTANPLAPSEQYVMDIDTTQNFNSPLKVEKVVTSVGGVLEFDPGISYMDS
                     VVYYWRVGTVPTGGGAPTYNFSSFTYIDPAHSGVGYNQSHYFQHLGSTGTNLRIMPTR
                     LWAFDSINHDIYQRNAVFPIAG"
     gene            complement(3197..4336)
                     /locus_tag="VGQ51_16915"
     CDS             complement(3197..4336)
                     /locus_tag="VGQ51_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEV9038321.1"
                     /translation="MLKRMRGRLLYCSLLLAGSLVHPTASAQFTEYSNEFLNIGAGAR
                     GLGMASAQVASVSDATAGYWNPAALAGIRGNPQLSLMHAEYYAGIGKYDFAALALPLK
                     DNKRTLGLTLLRFAVDDIPNTIFLVQPDGTVNFANITTFSSADYAFIVSLAQQSKMVG
                     DKKIDFGVNAKIIHRTAGDIATAWGFGFDAAMQMQGRRWKLGVVAKDVTTTFNAWSYS
                     LNEAMREVFYETQNEIPGKSIELTAPQLLVGGSYNFRLGKKLNLLAEGDLDVTFDGRR
                     NTVVSTKPVSIDPRVGLELSYKDVFFVRGGINNFQRVLDDRDTLNQKKIWIYQPSVGA
                     GFKVGDVQIDYAFVNLANQSYPLYTHVFSLRVDLKSKEEKKKQRR"
BASE COUNT         1070 a         1321 c         1120 g         1070 t
ORIGIN      
        1 cccgcaatag ggaagacggc attacgctgg taaatatcat gattgatcga atcgaatgcc
       61 catagacgcg taggcattat ccttaagtta gtgccggtgg acccgagatg ttgaaaataa
      121 tgggactggt tataccctac cccgctatgc gcgggatcga tataggtaaa agaagaaaag
      181 ttgtaagtag gtgcacctcc accggtcggc accgtaccga ccctccagta atagaccaca
      241 ctgtccatat agctgatgcc cgggtcgaat tccagcacgc cgcccaccga ggtaaccacc
      301 ttctccactt tcaacggcga attgaaattt tgggtggtgt cgatatccat cacatattgc
      361 tcgcttggcg ccagcggatt ggccgtcgaa gcatacagtt tcgaagtcgg cgtattgatg
      421 atggcataat tgtaaggata caccggattg gcttcgtcct ggtagatgaa gacgtccgtg
      481 gtaacggtat tgttggcata ggtcgcttcc gggatgacat tccccggatc gatcgtaacc
      541 gttatcttgt tatctccctt atcccggatg ccgactatcg gcacggagat atcgatggaa
      601 tcggaatacc ggatacccct gatcttcttg ctcagcagcg ttatgatgtt gccgttcggg
      661 taagtgcgtg tcaccagcac cccgacggaa tccgatactg ccttacccag gttataaaat
      721 cgcgccttca cctcgaacgt gctgttgctt atcgacacga agctcggatt gatcaccacc
      781 tgcgaggcct cgatatcata atcggtcggc ccctggttca gcttgagcac cgggtccccg
      841 tgcatcgtca tctcttcggc atgcagacgg gcgaaaaaat cccccggggc ggcagtcgtc
      901 aggtagttca acgcatcctt ttcgatgata ccgataggtt taccgtaatc gatcgtggtc
      961 atgtcctgga acagtccgct caacatgata ttgagatagt tgacgatccc caggtgcgtg
     1021 ctggccacaa acgcaatcgc gcctctctcc ttggacagca gccaatcctc ggacagcgtc
     1081 ttggacgacg tcagccgtga aacgtcatag acgaaaaagt cgccggcgtc gcaaccgttg
     1141 atatagaaca ccgggtattt tcctcccgtg ttgttataat cggttggttc gttaaggtta
     1201 taggccagca catcattggc cgagtgaccg aaataacaga gctcggtgat accggtcgag
     1261 aacagatcgg ccagaagcgt ggagggtatc tgcgtcaccg cggtggcatt cccgtcgcaa
     1321 aaggtactga tggtggctcc cattagcgtg tcgctgatca gtgagccgta ataattcata
     1381 tagttacaca ggatcgtacc gagatatggt tcactgacac ccgtaagatg taccccattc
     1441 ttcatccaca acctgccgtc gatcgtgttg ggcgcagaaa cctgcaggtt ctcgtattcc
     1501 ttcactttgg taagataggt ctctatctcc gcgcccgaca cgacagaaag ccggccgatc
     1561 ggcgtcagcg gcgtagcacc agccccgtca tttgcgctca atttgttgtc ggatgccgga
     1621 tatccgaagg taggtaccag gtccagcaga tcggcattag ggtcatgatt cacctcactg
     1681 gcggcattat aatcgttata acacatgcca tgcccgatga taaagatgaa ctgcggctta
     1741 accgtgaact tcgcccgcgc ccacctaatg aaattctgca ccgcatccgg gtgtttcttg
     1801 atgccaaacc cgaattgatc gaccagctca ttgatatcgt agatctgcac atcccagctg
     1861 ccgccggccg gactgctccg atacgtttta tagtcgttga ccggattgat cccgtcgttg
     1921 gtaccgtcat tgtaaagcaa ggggttggtg atgatcaggt aattgccctg caccgacgag
     1981 ttggcaaaat tttgaaaggt cttctgcgtc agcgtcgaaa cggcatgcac cgtggacgga
     2041 tcttcattca ccagcacgag attatacgtg gctgtcgaac cattcaacgc aaaactgacc
     2101 gtcgttccgc tgaccactcc ggtataccgc aacccgttcg taaggtcata taataccggt
     2161 gtagcacctg ccactatccc gaaattggcg atattgagca gataacccgc ggtacgcgcc
     2221 ggcagctgga acgcaaaatt atcctgaccg ccaaaattga attgccgcgg atacgacagc
     2281 tcataaaagg aggctaccat ccggtccgtc gtgaccgtcg agttgttgat ataattgatc
     2341 gttgccgtat tgccgctgat aaggctgagc ggcacgggct tggtggcctg cacgtcgtaa
     2401 aagctgttca tggtcgtatc ctccaccaca ctcccattta ccgtcagctg taccgtacgc
     2461 gcattgtccg ccgcgcctac cataccgaat tttatgctgg cattaggccc gccgggatag
     2521 acgaaaagat tgttcctggt gtccgttaaa ggagtgccgg gcggtatcgc cggagaagcc
     2581 cagaactccc cgatatcgta agaggaagaa tagatgtatt ctccgaccac ctgtgcaaac
     2641 ccggggttga cgccgccatt cttgaaatag ctgcccgtcg aatacataaa gaaaggctcc
     2701 ggcgtcagcg gactgcctgc aacgttattg gcgacatcgc tgaaatggaa ggtgcttccc
     2761 gtcgggttca ccgtcaaaaa ataaactgcc gtatccgttt ccaggctcca atgagtcgta
     2821 tgctgatacg aaggactgcg gtacaacggc gcatccggcg cgccgtcatt catcacgccc
     2881 cagaattcga taaagtccga tgcacccagc acgccgctcg atacgctcgt atagacagga
     2941 acctcctgac cattccggaa aagctggaag ttttgcaccg gcacagcgcc cattcccgca
     3001 gcagccagca ccgactgcgg gatacgataa agccccgtag cgccgacctt gaacttatag
     3061 taggtcttgg agtaatcgat ccattcatta ttgtacggct gagccgcaag ccgcagcgcc
     3121 gacaacaaga ttacgatcgt gtaaattttt ttcatacgct taggttttca gcgggcattc
     3181 ggccagctcg ggtacctcat ctcctctgtt tctttttctc ctccttgctc ttcagatcca
     3241 ccctcaacga aaatacatgt gtatacaatg gatatgactg attggccagg ttgacgaagg
     3301 cataatcgat ctgcacgtct cctactttga accccgcgcc tacactgggc tgatagatcc
     3361 agatcttttt ttgattcagc gtgtcgcggt cgtccagcac ccgctggaaa ttgttgatcc
     3421 ctccccgcac aaagaacaca tccttgtagg acagttccaa cccaacccgc ggatcgatgc
     3481 tcaccggttt cgtgctgacc accgtattcc ggcggccgtc gaaggtcacg tccaggtcac
     3541 cctcggccag gagattcagc tttttcccca gccggaaatt ataagacccg cccaccaaca
     3601 gctggggagc ggtcaattcg atcgattttc cgggtatctc attttgcgtc tcatagaata
     3661 cctcgcgcat ggcttcgttc agactgtagg accaggcatt gaaagtagtc gtcacatcct
     3721 ttgcgacgac acccaacttc caccgccgcc cctgcatctg catggccgca tcgaacccga
     3781 aaccccaggc cgtcgcgata tctcctgccg tccgatggat gatcttggca ttgacgccga
     3841 aatcgatctt cttgtctccg accattttcg actgctgcgc aagcgaaacg ataaaagcat
     3901 aatccgccga cgagaaggtc gtgatattgg cgaaattgac cgttccgtcc ggctgcacca
     3961 ggaatatcgt attgggaata tcgtccacgg caaaacgcaa taacgtcaac cccagcgtcc
     4021 gcttattatc cttcaacggc aacgccagcg ccgcaaaatc atacttgccg atgccggcat
     4081 aatattcagc atgcatcagg ctcagctggg gattaccacg gatacccgct aacgccgccg
     4141 ggttccagta accggccgtg gcatccgaca ccgaagccac ctgggcactg gccatgccca
     4201 acccacgcgc ccccgcaccg atgttcagga attcattgga atattcggta aactgtgcag
     4261 atgcagtcgg gtgaaccagg gatccggcca acagtagaga gcagtacaat aacctacccc
     4321 tcattctttt caacataagc caacagttaa ggcattttct cgatcggatc attagattta
     4381 gggactttat gtcgggcctt cggccaacgg gccgtggcct ttcggccatc tacgtataac
     4441 gggcttttac tcacaatatt gtaccattaa catgattccg ccccaacgca agttgccgga
     4501 ggaatccccc tccttttgcg ccccttcccc ttccgccccc caatcattcc ccgaatcccc
     4561 ctccttttgc gccccttccc c
//
LOCUS       DASYSV010000543         8101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2417058, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000543 DASYSV010000000
VERSION     DASYSV010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417058"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VGQ51_16920"
     CDS             <1..1201
                     /locus_tag="VGQ51_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HEV9038322.1"
                     /translation="WGAWLISLGDYMFNILHFDGKQVGSIYGTMGIASIFTPALFGIV
                     ADRWMNAERVLGLCHIIAAVMLIWASTLTSFGLFYMAMLFNSFFFMPTIALNNTVSYI
                     ILERRGFNIIRDFPPIRVWGTVGFICAMWVVDLCGWKQSPVQLYVSAAAGVTLGIYAF
                     TLPACFPIRTGPKPSLYASLGLDAFVLFKRPKMLIFFIFSMLLGFALQISNAFADGFI
                     SDFAHVDRYAGAFAVRHSVLLISISQISETLFILTIPFFLRRFGIKAVILMSIFAWVF
                     RFGLFGIGNPGSGLSFLILSMIVYGMAFDFFNISGSLFVEREADIKIRASAQGLFMVM
                     TNGFGAWAGTFLSGLVVDHFTENKVRDWHSIWLTFAGYALVLGIVFPLVFRYKHTAMD
                     DKAPVLQ"
     gene            1206..2192
                     /locus_tag="VGQ51_16925"
     CDS             1206..2192
                     /locus_tag="VGQ51_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038323.1"
                     /translation="MRWIYTFVTLISFGPAVSRAQPQADISNGIVHARFYLPDTATGY
                     YRATRFDWAGVMPLLEFRGHRYSGQWNPVYSPTVNDAIMGPVESFWPLGYDRAPAGGT
                     FVQIGVGVLTRPDTARYSSFRYYHIEDAGRWRITHRKNSIGFRQRIDGSSYRYVYTKT
                     VTLPKGESGMVIAHELRNTGRDTIETNVFDHNFFVIDGHDLGPGFGLRFRFHLDTSGS
                     RGARDLAAIQGDSIVILRPFATKETVFAVLKGYDSTAADYDLVLENPLTGSSLHIRGD
                     RPLVRLVYWGYHNTLCPEPYIHIRVAPGQTFTWTLRYSFEPRVDSSAAKSTR"
     gene            complement(2439..3542)
                     /locus_tag="VGQ51_16930"
     CDS             complement(2439..3542)
                     /locus_tag="VGQ51_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HEV9038324.1"
                     /translation="MIRLFASTALLCLMMTACKKTPGTGQNNMTNPSLQVVFQDSTYQ
                     LTGVAISKDGRLFTSYPLWSTTYKNAVAEITHGNPEVPYPNTMMNSWAEGDSAMDKWV
                     CVQAVYVDANNNLWVVDPASPFQRGVYLNSQKLVEISLATNTVIRTYPLAGATDDQSY
                     INDVRVDASNNVAYLSNSSEGGIVVVDLNTGNARQVLQGNTSVIADQTYILTIDGKQI
                     NQADAPFYVNSDGIALTPDNSYLYYKPLSDHNLYRVPTADLRDTTLDPNVLGARVEQL
                     GTYTTTDGMIFDQAGNLYMGDLEQHRIIKLDKNLKLTTVVQDSRLIWPDSYAISTDGY
                     LYMSCSQINLGPDFNNGASKRTTPYTIYRIKLP"
     gene            complement(3692..4009)
                     /gene="trxA"
                     /locus_tag="VGQ51_16935"
     CDS             complement(3692..4009)
                     /gene="trxA"
                     /locus_tag="VGQ51_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV9038325.1"
                     /translation="MALELTDTNFQDKVIASDKLTVVDFWAEWCGPCRAIGPVIEELS
                     KEYDGKINVGKVNVDHNPQLSVNYGITSIPAILFIKGGKVVDKLVGAQPKSNFVKKIE
                     SHL"
     gene            complement(4103..7741)
                     /gene="dnaE"
                     /locus_tag="VGQ51_16940"
     CDS             complement(4103..7741)
                     /gene="dnaE"
                     /locus_tag="VGQ51_16940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV9038326.1"
                     /translation="MCKFSHLHTHTQFSLLDGAASIQSLYNKAIKDGMPALAITDHGN
                     MFGAFEFVSEAYKHKNEDGTLKVKPIVGCEFYVVEDRSRKAFTKDQKDERYHQILLAK
                     NKEGYQNLIKLTSLGYTEGMYSKYPRIDKELILRYHEGLIATTCCIGAYVPQTILHDG
                     EAQAEKEFKWWLDLFGEDYFIELQRHNIPEQEKINRVLLDFARKYNVPVIATNDSHYT
                     DREDYNAHDILLCINTGEKQSTPGYDDFVNDDATVKNRRFKFPNDQFYFKTTEEMKTL
                     FSDVPEAIDNTNLIVDRVEVLHLKKDILLPAFPIPAAFQIHKDSNLDQWEYLKHLTNE
                     GAKQRYSDLTPEIRERLDFELFTIKTMGFAGYFLIVSDFIKAGRDMGVFIGPGRGSAA
                     GSVVAYCIGITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDEGRAKVIEYVVGKYGK
                     NQVAQIVTYGTMAAKMSIKDVSRALDLPLPESNALAKLVPERPGIELKRVLHAPLTSK
                     EGEKSLEEKDGVGADDMVNVRLLRDIYNGSDIRSKVLHEAERLEGSVRNTGLHAAGII
                     IAPDDLMTILPVCTAKDSDLLVTQIEGSVIEEAGVIKMDFLGLRTLNILKTALELIKE
                     NHGIGIIIDDIPLDDRKTFELYQRAETIGTFQFESPGMQKYLKDLRPDKFDDLIAMNA
                     LYRPGPIAYIPNYIDRKHGREPIVFDLPEMEEFLGDTYGITVYQEQVMLLAQKLAGFS
                     KGDADVLRKAMGKKQKSILDKMKSQFVKGATAKGFNADTLEKIWTDWEAFAQYAFNKS
                     HSTCYAFVAYQTAYLKAHYPSEYMAAVLNHAGSIEKITFFMEECKRMGLKVLGPDINE
                     SKKGFAVNQKGEIRVGLGGLKGVGEAAVESIIEDRNQKGPFTNIFDMIQRINQRAVNK
                     KTLESLAYAGTFDCFPDIHRAQYFHMPQGDTSTGLEKIIKFGNVYQTNSTQSSNSLFG
                     DLVMPDIATPKIPNCEPWTLTELLDHEKDVTGMFMSGHPLDHFKFEITHYGITRLGEF
                     NEIKEALTLQSNPGKSLRLAGLVVDAQHRVSKTGKQFGSLVIEDYTGKAEFMLWSEDY
                     ARYSNYLEKGKNLFLTGGFRQRFNKSEFEFRIEKMMLLESIKPILTKQVIIEVAARFI
                     NEQIVQFMDQNMRKYPGKSGLKFNIIEPKTHSNISLFTMGSGFEMNDEMTAFLEKTPE
                     LEVQVVTI"
BASE COUNT         1693 a         2092 c         2164 g         2152 t
ORIGIN      
        1 ctggggggcc tggctgatct cattgggaga ctatatgttc aatatacttc atttcgacgg
       61 taagcaggtc ggcagcatct atgggacgat ggggatcgcc tcgatattca cacccgcatt
      121 gttcgggatc gtcgcggacc ggtggatgaa tgcggaaagg gtgctggggc tttgtcatat
      181 tattgcggcg gttatgctga tctgggcctc cacactgacg agctttggcc ttttctacat
      241 ggcgatgctt ttcaactcct tcttctttat gcccacgatc gcgctgaaca acaccgtatc
      301 ctatatcatt cttgaaagaa gagggttcaa tattatccgg gattttcccc cgatccgtgt
      361 ctggggtacg gtgggcttta tctgtgcgat gtgggtcgtc gatctgtgcg gctggaaaca
      421 gagcccggtt cagctgtatg tgagcgccgc tgcgggtgtg acgctcggca tctatgcctt
      481 caccctgccc gcgtgttttc ccatacggac agggcccaaa cccagtcttt atgcatcgct
      541 ggggctggat gcctttgtcc tgttcaagag accgaaaatg ctgatctttt ttattttttc
      601 gatgctgttg ggttttgcct tgcagatcag caatgccttt gcggatgggt tcatcagcga
      661 ttttgcgcat gtcgaccgtt atgccggggc ctttgccgtc aggcattcgg tgctgctcat
      721 ctccatttcg cagatctcgg agactttatt cattctgacc attcctttct ttttacgacg
      781 atttggcatt aaagcggtga tcctgatgag cattttcgcc tgggtattcc ggtttggact
      841 cttcggtatc ggcaaccccg gcagcgggct gtctttcctg atcctgtcga tgatcgttta
      901 cgggatggcc ttcgacttct tcaatatttc cggatcgctg ttcgtcgagc gggaggccga
      961 tatcaagatc agggccagcg cccaggggct cttcatggtg atgacgaacg ggttcggcgc
     1021 ctgggcagga actttcctga gcgggttggt ggtcgatcat ttcactgaaa acaaagtgcg
     1081 ggactggcac agcatctggc tgacctttgc cgggtatgcg ctggtgctcg ggatcgtgtt
     1141 cccgctggta ttccggtata agcatacagc catggacgac aaggctcccg tcctgcaata
     1201 gctatatgag gtggatatat accttcgtaa cgctgatctc cttcgggcct gccgtctccc
     1261 gggcccagcc gcaggccgat atcagcaatg gcatcgtgca tgcgcggttt tatttgccgg
     1321 ataccgcgac aggttattac cgcgctaccc gttttgactg ggcgggcgtc atgcccctgc
     1381 tcgaattccg gggacaccgg tatagcggcc agtggaatcc cgtttattcc ccgaccgtca
     1441 acgacgcgat catgggccct gttgaatcct tttggccgtt gggatatgac cgcgcgccag
     1501 caggcggaac attcgtacag atcggcgtcg gggtcctcac gcgaccggat acagcccgtt
     1561 attcctcctt ccggtattat cacatcgaag acgccggacg gtggcggata acacaccgga
     1621 agaacagtat cggattcaga caacgcatcg acggttcgtc ctacagatat gtatatacga
     1681 agacggtcac gctgccgaaa ggtgagtccg gaatggtgat cgcgcatgag ctacgcaata
     1741 ccggccggga tacgatcgaa accaatgtct tcgaccataa tttttttgtc attgacggtc
     1801 acgacctggg accgggtttc gggctccggt tccggtttca tttggacacg tcagggtcgc
     1861 ggggcgcgcg cgatctggcg gccatacaag gcgattcgat cgtcatcctg cgaccttttg
     1921 cgacaaaaga gaccgtcttt gccgtgctca aaggttacga cagcaccgct gccgattacg
     1981 atttagtttt agaaaacccg ctgaccggct catcgctgca tatccgcggc gacagaccgc
     2041 ttgtccggct cgtttattgg ggatatcata acacactgtg ccccgaaccc tatatccata
     2101 tcagggtggc gcccggccag accttcacgt ggacgttgcg ttatagcttc gaaccgcggg
     2161 tagatagttc tgctgcgaaa agcacccgct gaatccgcca tcaacggtaa tgcggcatat
     2221 gaaaaggaga gggtggatgc aagcagtaaa aacagcagca agggtcgcat tagcaggggt
     2281 attccgcccc aaaataggaa aaagtttcga taaaaaaagc ctgggaccgg ggggcggcag
     2341 ggcgaaaaaa agcccgggac cgggggacgg cgggacgaaa agaagcccgc atccttgaag
     2401 cagcgggctt atccaaagca taacgatcaa cgccggggct agggtaactt gatccggtaa
     2461 atggtatagg gtgtcgttcg cttgctggcc ccattgttga agtcgggtcc cagattaatt
     2521 tgcgaacaac tcatgtacaa ataaccatcg gtggagatag catagctatc gggccagatg
     2581 agccggctgt cctgcacgac ggtagttaac ttaaggttct tatcgagttt aatgatacgg
     2641 tgctgttcga gatctcccat atacagattg cctgcctggt cgaagatcat tccgtccgtg
     2701 gtggtatagg tacccaattg ttccactctt gccccgagga cattcggatc cagtgtcgta
     2761 tcccgcaggt cggcagtggg tacccggtac aggttatgat cggacagagg tttatagtac
     2821 agataagaat tgtcgggcgt cagcgcgatc ccgtcggagt tgacataaaa aggtgcgtcg
     2881 gcctggttga tctgcttacc atcgatggtc aggatatagg tctgatcggc gatgaccgag
     2941 gtgttaccct gcaggacctg gcgggcgtta cccgtattga gatcgacgac gacgataccg
     3001 ccttcgctgg aattgctcag gtaggcgaca ttgttggagg catcgacccg gacatcgttg
     3061 atatagcttt gatcgtccgt ggcgccggcc agcggatagg tgcggatgac ggtattggta
     3121 gccagactga tctctacgag cttctggcta ttgaggtata cgcctctttg aaaaggcgat
     3181 gccggatcga cgacccagag attgttgttg gcatcgacat agacggcctg aacgcaaacc
     3241 cacttgtcca ttgcgctgtc gccttccgcc cagctgttca tcattgtgtt gggatagggt
     3301 acttcgggat tgccatgggt aatttcggcg actgcattct tgtaggtggt ggaccataaa
     3361 gggtatgaag tgaacaaccg tccgtctttc gaaatggcaa cccccgtcag ctgataggtg
     3421 ctatcctgga aaacgacctg tagggacgga ttagtcatat tgttctgtcc ggttccgggt
     3481 gttttcttac aggcagtcat cataaggcat aggagcgcgg tggaggcgaa aagtcttatc
     3541 ataaagaggc tttacacttt ctttaacata aagcgtaccg tgccttttta agcttgcaca
     3601 ggggaagaaa ttctacagga acgtagcaaa aagcccgcac ggagcgcggg cttttgggga
     3661 tatattgact tttcgaccct gaaggaccta cttacaggtg cgattcgatt ttcttgacaa
     3721 aattggactt aggttgcgcg ccgaccagct tgtcgacgac cttgcctccc ttgatgaaaa
     3781 ggatggccgg gatgctcgta atgccataat tgaccgagag ttgcggattg tgatccacgt
     3841 tcaccttacc cacattgatc ttaccgtcgt attccttcga caattcttca atgaccggtc
     3901 cgatagcgcg gcagggtccg caccattccg cccagaaatc tacaaccgtc aatttatcgg
     3961 aggcgatcac cttgtcctga aaattagtat ctgttagttc tagagccata atcgggtgtt
     4021 ttatatgata gttatgtttt ttgaattaca aatgtcggct taattaagca actttttcgt
     4081 cggatgcatg acaaatttag catcaaatgg tcacgacctg gacctcgagt tccggagttt
     4141 tttcaaggaa ggcggtcatt tcgtcattca tctcaaatcc gctgcccatg gtgaacagac
     4201 tgatgttgct atgcgttttc ggctctataa tattgaattt caagcctgat ttgcccgggt
     4261 atttccgcat gttctgatcc atgaattgca cgatctgttc attaatgaac cgggccgcta
     4321 cctcgatgat cacctgtttg gtcaggatag gctttatact ctcgaggagc atcattttct
     4381 ctatccggaa ctcgaattcc gatttattga acctttgcct gaagccaccc gtcaggaaca
     4441 ggttcttgcc cttttccagg tagttgctat agcgcgcata atcctcgctc cacaacatga
     4501 actcggcctt cccggtataa tcttctatca ccagcgatcc aaactgtttg ccagtcttgc
     4561 tgacacgatg ttgcgcatcc acgacgagac ccgcgagtcg caaagacttt ccgggattag
     4621 attgcagcgt caacgcttct tttatctcgt tgaactcgcc cagtcgcgtg ataccataat
     4681 gcgtgatctc gaatttgaaa tgatccagcg gatgtccgct catgaacatg cccgtgacgt
     4741 ccttctcatg atcgagcagc tcggtcagcg tccacggttc acagttaggg atcttcggag
     4801 tggcgatatc gggcatgacc aggtctccga agaggctgtt gctgctttgg gtagaattgg
     4861 tctgatagac attcccgaat ttaatgatct tctcaagacc cgtcgacgta tctccttgcg
     4921 gcatatggaa atattgcgcg cggtggatgt cggggaaaca atcgaatgtt ccggcatagg
     4981 ccaggctttc cagcgttttt ttgttgaccg cgcgctggtt gatccgttgg atcatgtcga
     5041 agatattggt gaacggccct ttctgattcc gatcctcaat gatgctttcc accgccgcct
     5101 cgccaacgcc tttcagaccg cccaatccga cacggatctc gcctttctga ttgacggcga
     5161 accctttttt cgattcgttg atatcaggcc ccaatacttt tagtcccatc cgtttgcact
     5221 cctccatgaa gaaggtgatc ttttcaatgc tgccggcatg gtttagtact gctgccatat
     5281 attcggaagg gtagtgcgcc tttaggtacg ccgtttggta ggcgacaaaa gcataacagg
     5341 tggagtgtga tttgttgaag gcgtattgcg cgaacgcctc ccagtccgtc cagatctttt
     5401 cgagtgtatc cgcattgaac ccttttgccg tggctccctt cacgaactgg ctcttcatct
     5461 tgtcaagaat ggatttctgt tttttcccca tggcctttct cagcacatct gcgtccccct
     5521 tgctgaagcc cgctagtttc tgcgccagca gcatcacctg ctcctgataa acggtgatac
     5581 cataggtgtc gcccaaaaat tcttccatct cgggaagatc gaacacgatg ggctcacggc
     5641 catgcttacg gtctatatag ttggggatat aagcgatggg acccgggcgg tacagcgcat
     5701 tcatcgcaat gagatcgtcg aacttatcgg gcctgaggtc tttcaggtat ttctgcatac
     5761 cgggactttc gaactggaag gtcccgatcg tctcggcacg ttgatatagc tcgaatgttt
     5821 tgcgatcatc cagcggaatg tcgtcaatga ttatcccgat gccgtgattc tctttgatga
     5881 gctcgagcgc cgtcttgagg atattcaggg tcctgaggcc taagaagtcc attttaataa
     5941 ctccggcttc ctcgatgacg ctgccttcga tctgggtgac gagaagatcg gaatcttttg
     6001 cggtacagac gggtaagatc gtcatcaggt catccggcgc gatgatgatc ccggctgcat
     6061 gcagcccggt attacggacc gatccttcca atctttccgc ctcatgaagc accttgctcc
     6121 ggatgtcgct gccattgtag atatcgcgca aaagacggac attcaccata tcatccgcgc
     6181 cgacgccgtc tttttcttcg agcgatttct cgccttcttt tgacgtgagc ggggcatgca
     6241 ggacccgttt caattcgatg cctggtcttt cagggaccag tttggcgagg gcattggact
     6301 cggggagggg cagatcgagc gccctggaca cgtccttgat gctcattttg gccgccatgg
     6361 taccgtaggt gacgatctgt gccacctggt tctttccata ttttccaacg acgtattcga
     6421 tcacctttgc gcgtccctca tcgtcgaagt cggtgtcgat atcgggcatc gatttacgat
     6481 cggggttcag aaaacgttcg aaaagcaggt tgtacttgat ggggtcgatg ttggtgatgc
     6541 cgatgcaata ggccaccacg ctgccggcgg ccgatccccg ccccggacct ataaaaaccc
     6601 ccatatcccg tcctgccttt atgaaatcgc tcacgatgag gaaatagccc gcaaaaccca
     6661 tggtcttgat cgtgaaaagt tcgaaatcca gacgttcccg gatttcgggt gtcagatcgc
     6721 tgtaccgttg tttggcgccc tcattagtca ggtgtttgag atactcccac tgatccagat
     6781 tgctgtcttt gtgtatctgg aaggcggcgg ggatagggaa agccggaagg agaatgtcct
     6841 ttttcagatg gagcacctcg acgcggtcga cgatgagatt ggtattgtcg atggcctccg
     6901 gcacgtcgct gaacagggtc ttcatttcct ccgtcgtctt gaaatagaac tggtcgttcg
     6961 ggaatttgaa acgccggttc ttcacggtcg cgtcgtcgtt cacgaaatcg tcgtaacctg
     7021 gcgtgctctg tttctccccg gtattgatac acaacaggat atcgtgggcg ttgtagtctt
     7081 cgcggtcggt atagtggctg tcattggtgg cgatcaccgg tacgttgtat ttgcgggcaa
     7141 agtcgagcag gacccggttg atcttctcct gttcgggaat attgtgccgc tgcagctcga
     7201 taaaatagtc ctcgccgaaa aggtccagcc accatttgaa ctctttttcc gcctgtgcct
     7261 cgccgtcatg caggatggtc tgcggaacat aggcgccgat acagcaggtg gtcgcgatca
     7321 gtccttcatg ataacgaagg atcagttcct tgtcgatacg cggatatttg ctgtacatac
     7381 cctcggtata ccccagggag gtcagcttga tcaggttctg atagccctct ttgttcttcg
     7441 ccagcaggat ctgatgatag cgttcgtctt tctggtcttt ggtgaaggcc ttgcggctcc
     7501 ggtcttcgac gacatagaac tcgcaaccta cgatgggctt taccttgagg gtgccgtcct
     7561 cgttcttgtg tttataagcc tccgaaacga attcgaaggc gccgaacatg ttgccgtggt
     7621 cggtgatggc cagggcgggc ataccatctt ttatcgcctt gttatagagg ctttggatgg
     7681 aggcggcgcc gtccaaaaga gaaaattgcg tatgggtatg tagatgagag aatttgcaca
     7741 taggacctgc aattaacaat aacattcggt gaggggaatc gttcattggg cacaatcttt
     7801 ccacaggtgg gtcgccgggg gcggtttgca ggcccctccg ccttctgcgc atggctcccc
     7861 gccgctcttc gccgcccttc gcgcatggct ccccgccact cttcgccgcc ctccgcgcat
     7921 ggctccccgc cgctctttgc cactcgccgc agccgtccgc caaattggca atatttttgc
     7981 gatttttgac tatctttggc cctatgccaa tatcctccat gttcataata tgttgaaaaa
     8041 tctcataccc ataacgttta tcgtttgttt tgcctctggc tgcagcacat tcaaacccgc
     8101 t
//
LOCUS       DASYSV010000544         5259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2157709, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000544 DASYSV010000000
VERSION     DASYSV010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2157709"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..948)
                     /locus_tag="VGQ51_16945"
     CDS             complement(205..948)
                     /locus_tag="VGQ51_16945"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HEV9038327.1"
                     /translation="MKKLMIAAAGRRIAGGALIFAASTASGQFAGKLVYQIVRPSVVV
                     TMTYYQSGNSVHVEAYTIDVTKGKLDSSTFFPQDTILFDLTKGTETHLQRHTGRAIIT
                     QYTATIMAEAGYGKGISSASVQLVGPDTANGYSCTHFVENYGGAKPFGASRRDIWVTN
                     ALGNPGIMVMGSFLYYTPGAQRLQALNTAGCAGVVVRTKMSGMGQTTVMNLIAVDTKR
                     LNARMFQIPSYYSVIDNSGYVPPKAVTKP"
     gene            complement(1036..1551)
                     /locus_tag="VGQ51_16950"
     CDS             complement(1036..1551)
                     /locus_tag="VGQ51_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HEV9038328.1"
                     /translation="MENYRNIGRLVAVFGVQGEMVLQHRLGKKTSLKGLKTLFLEERK
                     DELLPWFVQSTRIKGPEEVFVKLEGLDTKEAAKRMVRKEVWVREQDFSGIAGRSAPIS
                     LVGFRLFDGVSDLGEILEVIEQPHQVLCRIDLGGKEALIPLHEETLRGVDQKKREVYV
                     ELPDGLLDVYR"
     gene            complement(1602..2087)
                     /gene="rpsP"
                     /locus_tag="VGQ51_16955"
     CDS             complement(1602..2087)
                     /gene="rpsP"
                     /locus_tag="VGQ51_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV9038329.1"
                     /translation="MPVKIRLQRHGSKKRPFYFIVVADARAPRDGKFIQKIGTYNPLT
                     VPASVQLDRQKALDWLHKGAQPTDTVRRILSYKGVLYLKHLLRGVKLGLFDDAAAMEK
                     FQKWHGEHELQMKKRQEENRKTRPGGGGRRGPGGPPMRRMERPERTERKPEGGAATTE
                     A"
     gene            complement(2450..3766)
                     /gene="ffh"
                     /locus_tag="VGQ51_16960"
     CDS             complement(2450..3766)
                     /gene="ffh"
                     /locus_tag="VGQ51_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV9038330.1"
                     /translation="MFESLSERLESAFKQIKGEGRITELNIAATVKDIRRALIDADVN
                     FKIAKEFTDTVKDTALGQKVLTAVSPGQLMVKVVKDELVKLMGGTESELNAKGNPAVI
                     LVAGLQGSGKTTFSGKLANYLKGRKGLSPMLVADDIYRPAAIEQLRVLGEQIGVEVYS
                     EPENKDAVDIARNAIREARSRNKNVVIIDTAGRLAIDEAMMQEVAAIKGAVNPGEILF
                     VVDSMTGQDAVNTAKAFNDRLDFSGVVLTKLDGDTRGGAALSIKYTVNKPIKFISSGE
                     KMDTLDVFYPERMAQRILGMGDITTLVEKAQAQFDEAEARKLEKKIRKNQFDFEDFKQ
                     QLQQIKKMGNLKDLMGMIPGVGKAIKDIDISDDSFKGIEAIINSMTPFERNNPDVIDL
                     SRRKRLAKGCGKDINEVNAFLKQFDQMKQMMKMMNKMPMGAAMRRR"
     gene            complement(3894..4763)
                     /locus_tag="VGQ51_16965"
     CDS             complement(3894..4763)
                     /locus_tag="VGQ51_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEV9038331.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVELISPEEEVKLAIRIK
                     QGDQKALDKLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKVGLTNRIQKAYSQLEQEFEREPSAEELAE
                     ILELDIEEVSSTLGIAARHVSMDTPLSEGEDNTLIDVLENPNAELANSNIEHKESLKQ
                     EIDRSLKTLTERQKEVICFFFGIGVDHPMSLEDIGEKFNLTRERVRQIKDKAITKLRS
                     NSRSKMLRSYLGI"
BASE COUNT         1115 a         1564 c         1191 g         1389 t
ORIGIN      
        1 taagatcatt aacagccccc atccatattt tccagctggc agcggcatcg gatcgaattg
       61 cgttcaagtt acacattttc ccccgccccg catccgcagc tcgccccagc gctctatccc
      121 ccggcccgca acctcacccc gccgcgacac tctatccctc actccacaac ctcgccctgc
      181 cgcgaccgcc ccaccactcc ccgcctacgg cttggtaacc gctttcggcg gcacatatcc
      241 gctattgtcg atgacagaat aatatgacgg tatctggaac atccgcgcat taagcctttt
      301 cgtatccacg gcgatgaggt tcataacggt ggtctgtccc atcccgctca ttttggtacg
      361 gacgacgaca cccgcgcaac ccgccgtatt cagcgcctgc agccgctgtg cgcccggcgt
      421 ataatacagg aagctcccca taaccatgat gcccggattg ccgagagcat tggtcaccca
      481 gatgtcgcgc cggctcgccc caaaaggctt ggcgccacca tagttctcga caaaatgtgt
      541 acagctataa ccgtttgccg tatccggccc caccagttgc acggaggctg aagaaatgcc
      601 ctttccatag cccgcctctg ccatgatcgt cgccgtatac tgtgtgatga tagcgcggcc
      661 cgtatgcctc tgcagatggg tctccgtgcc cttcgtgaga tcgaaaagga tcgtgtcctg
      721 gggaaaaaaa gtgctcgagt ccagcttgcc cttcgtaacg tcgatggtgt aagcctcgac
      781 atggacacta ttgccacttt ggtaataggt cattgtcacg accacactgg gccgaacgat
      841 ctggtatacc agtttccctg caaactgtcc actcgcggta ctcgcggcaa atatcagggc
      901 gcccccggcg attcttctgc cggcggcggc gatcatcagc tttttcatag ccaggcattt
      961 cctgcaagct atcccgcggg cggacatctg gcggcgcgat tccgtatctg gcggcagcgg
     1021 gcttgtatat ggcaattacc gatacacatc gagtagtcca tccggcagct ccacatacac
     1081 ctcgcgtttt ttctgatcca ctccgcgtag cgtctcttca tgcaacggga tcagcgcctc
     1141 cttgccccct aaatctatcc ggcataacac ctggtgcggc tgttcgatca cctccagtat
     1201 ctcccccaga tccgagaccc cgtcgaagag acggaaaccc accagcgaga tcggcgccga
     1261 cctgccggcg atccccgaaa aatcctgctc ccttacccaa acttctttac ggaccatccg
     1321 tttcgccgcc tccttcgtat ccagaccctc taatttcaca aacacttcct ccggcccttt
     1381 gatccgcgta ctctgcacga accagggcag cagctcgtcc ttcctttctt cgaggaacag
     1441 cgttttaagc cccttcaggc tggtcttctt gcccagccgg tgttgcagta ccatctcgcc
     1501 ctgcacgccg aatacagcca ccagccggcc gatgttacga tagttctcca taaaaaacag
     1561 gaggccgttg ggcctcctgc atgaattaaa tttaaaaaga attatgcttc cgtagtcgcg
     1621 gctccgcctt cgggcttcct ttccgtacgc tcgggacgct ccatccttct catgggagga
     1681 ccgccgggac ctctcctgcc accgccaccg gggcgtgtct tgcgattttc ttcctggcgt
     1741 ttcttcatct ggagctcatg ctcgccatgc catttttgga atttctccat ggcagcggcg
     1801 tcatcgaata accccagctt aacacctctc agcaaatgtt tcagatagag aactcctttg
     1861 taggaaagaa tgcggcgaac ggtgtccgtc ggctgcgcac ctttgtgcaa ccaatccaac
     1921 gccttctggc gatctagctg gacggaggca gggactgtca aaggattgta agtgccgatc
     1981 ttctggatga attttccatc tctgggtgca cgggcgtcgg ctactacgat gaagtaaaaa
     2041 ggacgtttct tagacccgtg acgttgcagt ctgattttaa ctggcatctt aaatagaaat
     2101 ttaaatgcgt gaaataataa ggttgccttc caatttgcac cccaaactat aaggttcggg
     2161 gtttttgttt tgggattgca aaactagcaa tccgatatca catttcaaaa ataatacttc
     2221 atgttttaaa gagctagtgt tgcagcgaaa ttttttactc acggttccct ctccccaatc
     2281 cactctccta atcaaatccc gcgccaaccc tgccggcccc ctgcaaacga ttgcaaccga
     2341 acgctctctg caacaacgtt ttagcaaacc tgctcccatc ctgcattcgc cccgccgaaa
     2401 cctgaattct ccccacaaaa ttccgcgttc gtcccgcttc ccgcgcccgt tatctccttc
     2461 tcatcgcggc ccccatcggc attttgttca tcattttcat catctgcttc atttgatcga
     2521 attgcttcag aaaagcattc acctcattga tatcctttcc gcaacccttc gcgagcctct
     2581 tgcgtctgct caggtcgata acgtcgggat tgttccgctc aaaaggcgtc atcgagttga
     2641 tgatggcttc gatgcccttg aacgagtcgt cgctgatgtc gatatccttg atcgcctttc
     2701 cgacaccggg tatcatgccc atcaggtcct tgagattccc catcttcttt atctgctgta
     2761 actgttgctt gaaatcctcg aaatcgaatt gatttttgcg gatttttttc tccagtttcc
     2821 tggcctcggc ttcgtcgaac tgcgcctgcg ccttttcgac gagggtagtg atatccccca
     2881 tccccaggat ccgctgcgcc atacgctcgg gatagaatac gtccaacgta tccattttct
     2941 cgccgctgct gatgaatttg atcggtttgt tgaccgtata cttgatcgac agtgcggcgc
     3001 caccccgggt gtcgccatcg agctttgtca ataccacgcc cgaaaagtcc agccggtcgt
     3061 taaaggcctt cgccgtattc accgcatcct gacccgtcat cgagtccacc acgaacagaa
     3121 tttctccagg attgaccgcc cccttgatcg ccgccacttc ctgcatcatg gcctcatcga
     3181 tggccagacg gcccgccgta tcgataatga cgacattctt attcctggac cgggcctcgc
     3241 ggatggcatt tcgtgcgata tcgacggcgt ccttattctc gggttcgctg tagacttcga
     3301 cgccgatctg ctcacccaat acccgcagct gttcgatggc cgcgggacga tagatgtcgt
     3361 ccgcaaccag catgggcgac aaccccttcc ggcccttgag ataattggcg agctttccgc
     3421 tgaaagtcgt cttaccgcta ccctgcagac cggcaacgag gatgactgcg ggattgcctt
     3481 tggcattgag ctcgctttcg gttccgccca tcagcttcac cagctcgtcc ttgacgacct
     3541 tgaccatcag ctggcccggg gagacagccg tgaggacctt ctgccccagc gctgtgtcct
     3601 ttaccgtgtc ggtgaattct ttggcgatct taaagttgac gtccgcgtcg atcagcgcgc
     3661 ggcgtatgtc ttttaccgtt gccgcaatat tcagctcggt gatgcggcct tctcctttta
     3721 tctgtttgaa ggctgactcc agccgttcgg ataagctctc gaacataatt attttgtttg
     3781 gcgacccccc tccggtccaa aaaatgcaaa agtgaatgta aacatgttac attcactttc
     3841 gggagaacaa aaatacggaa atttaccggc cgcagccggc tctgagtgta aaattatatg
     3901 ccgaggtaac tccttaacat ttttgagcgc gaattgctgc gcagtttggt aatagcttta
     3961 tccttgatct gccggacacg ctcgcgcgtc aggttgaact tctcgccgat atcttccagg
     4021 ctcatcggat gatccacccc gatcccgaaa aagaagcaga tcacctcttt ctgccgctcg
     4081 gtcagcgtct tcagcgaccg gtcgatctcc tgtttcagcg actccttgtg ctcgatgttc
     4141 gaattggcca gctccgcatt gggattctcc agcacgtcga tcagggtatt gtcctcaccc
     4201 tccgacagcg gtgtatccat cgaaacatgc cgtgcggcga tgcccagtgt cgaggatact
     4261 tcctcgatat ccagctcgag tatctcggcc agctcttcgg cggaaggttc gcgctcgaac
     4321 tcctgctcca gctgcgaata agccttctgg atacggttgg tcagacccac cttattcagc
     4381 ggaagacgca cgatacgcga ctgctcggcc agcgcctgca ggatactctg acgtatccac
     4441 catactgcat aagaaatgaa tttgaatccg cgcgtttcat caaaacgttg ggccgccttg
     4501 atcaatccca ggttaccctc attgatgagg tcaggcaggg acaggccctg gttctggtat
     4561 tgcttggcta cggatacgac aaagcggaga ttggcttttg tcagtttatc gagagctttc
     4621 tgatctccct gcttgatacg gatggcgagt ttgacctctt cttcgggact gatcaactct
     4681 actttcccta tctcctgcag gtacttctcc aggctctgag attcacgatt cgttatactc
     4741 ttcgtgatct tcagttgacg catgctcata ggcagaggtc ttttaaaggg tttttaggtg
     4801 taaggctttt tgcttttaat agttccgcat cgtgcagacc tttcagtcgc ccttcagcgg
     4861 gttcattgga ttggctaaag tctcacctct ctgctgaggc tccggcgtct cctattttgg
     4921 gaaaatttaa atcggtttta gtcttggtaa aaaacgtttt tcttggcctt ttcttgtatc
     4981 atttggtcgt caactgattc cggtaggtat attcttccca atttaccgat tttgaaaagg
     5041 ataaccaatt tttttaataa actattgatt ctcacccata attggttaaa atttcattaa
     5101 aaattaccca tttgccgtca tgaaaatcac acgaaaaaaa attttgacgg ttgaattatt
     5161 tttttattat tgcacagagc agccccttgc gggactgcat aataacagcg gaggataaag
     5221 attcaaatgc aatagatgag taagaaacaa atttttacg
//
LOCUS       DASYSV010000545         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2033361, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000545 DASYSV010000000
VERSION     DASYSV010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033361"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VGQ51_16970"
     CDS             complement(<1..961)
                     /locus_tag="VGQ51_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9038332.1"
                     /translation="MSKKYSPVQNGSRREFLKKGLVAAAGFYIVPRHVLGGKGYRAPS
                     DRLVVAGVGAGGKGESDLSWLSATGKVDIAVLCDVDDRRSANMRKKFPSAKYYKDYRQ
                     MLEKEHKHIDAVNVATPDHMHAVIGMAAMQLGKHVYIQKPLAHDIYEVRMLTEAAPRY
                     KVVTQMGNQGASGDGVRQLVDWYRAGIIGDVTEVYCFTNRPVWPQGIKWPTAKATVPA
                     ELDWNLWLGTAPYREYVDNLVPFNWRGWWDYGTGALGDMGCHIIAPAFQVLELGYPTE
                     AQCSVGSVYTGEFTQGYFPDSCPPSSHIVLKFKGAGGKPDVTLH"
     gene            complement(1019..1405)
                     /locus_tag="VGQ51_16975"
     CDS             complement(1019..1405)
                     /locus_tag="VGQ51_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038333.1"
                     /translation="MNSPMVRILLAEDHTIIRKGMRQILTEALPQAQIEEIGEGEEWT
                     ERVMDRPWDVIISDHHGIGSRMPEDPDRPVLLLNLYTHEHHGYRALRTGITGILGRDA
                     AADELVWAVRQVLSGKKFLAIPSKNN"
     gene            1480..1728
                     /locus_tag="VGQ51_16980"
     CDS             1480..1728
                     /locus_tag="VGQ51_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038334.1"
                     /translation="MIFAYVYLLIHCHGFARLTIFYSLSRLCNADDLLLAVAALQGRF
                     DPGLVGIGKMSPKMEKEPSPVLADDQTGNFLLVCLAVA"
     gene            complement(1768..2073)
                     /locus_tag="VGQ51_16985"
     CDS             complement(1768..2073)
                     /locus_tag="VGQ51_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038335.1"
                     /translation="MRKTMLPLLIILAAFSACAQPSHLDEFYRKYQATEGTDKLSVDP
                     AFLFSASFNRDAADSDKDWTHRITSIRCLIIDGKKSTTGAHEWSDLGASLRADRFEE"
     gene            complement(2094..2612)
                     /locus_tag="VGQ51_16990"
     CDS             complement(2094..2612)
                     /locus_tag="VGQ51_16990"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HEV9038336.1"
                     /translation="MRKVKRHLLVLPLLLLCTGAFCQDDAIGRFFGKYLDDSRFTVVS
                     VSPKMFRLLSKVKWDTIPADLQQTVTRLQSFRIISTETTPMVFYKEALAAIDRRQYED
                     LIMLRSSKDNVRFMVRDDGKVIRELLMIAVDENGFTLMSFVGDIDLDKLSRLSSDMGI
                     KGMEDLKNAKRH"
     gene            complement(2616..3122)
                     /locus_tag="VGQ51_16995"
     CDS             complement(2616..3122)
                     /locus_tag="VGQ51_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038337.1"
                     /translation="MEKATVIALLEKYFEAGTTVAEEKVLAEYFRGQNDIDPDLAPYR
                     DLFLYFGQEAEVIAGPGFENRILQHVGLSGSPGLHASQGLHGPASSVRTFRLGFIAAA
                     AAICAIVAGLFLVTPAVRQAPPVVVATTITDTYDNPEQALAAVRHALLIASTHLNESR
                     RSITGLKN"
     gene            complement(3104..3622)
                     /locus_tag="VGQ51_17000"
     CDS             complement(3104..3622)
                     /locus_tag="VGQ51_17000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9038338.1"
                     /translation="MSEEAFKQHILPMKDKLFRFAYRFLQDVQEAEDAVQDVMAGVWA
                     RRAEWLQWKSIPAYCMTATRNNCLDRLRRRTTTTIREGAAAEISSPDRDPYEKVTNKE
                     MMRRIQECIDDLPPQQQHVIRLREMEGFSYNEIAEVLDMTLDQVKVNLFRGRSAIRKT
                     IIKEEEIWKRQP"
     gene            3675..>4498
                     /locus_tag="VGQ51_17005"
     CDS             3675..>4498
                     /locus_tag="VGQ51_17005"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEV9038339.1"
                     /translation="MKKYYSVAGRVSPVAALVLAIAGRVLPILVFILTAGSLHAQDPI
                     GLSDLRSAEKLFGLHFTPVKEDSILSGLDDHLRLYQYLHEHDLYNDVPLSLSFDPLLP
                     GTPYDRKQAPLQWDLPQNVTLPANRNDLAWYSIPELASLLRQKKISSVDLTTFFLDRL
                     KRYGPVLHCVIQLLEDSALAQAKRADADLAKGVYRGPLQGIPYGIKDLFAVKGSYTTW
                     GSPPYKGQTIEQDCYVAKRLAEAGAVLVAKLSLGELAMDDVWFGGLTRNPWKPQTGS"
BASE COUNT          946 a         1249 c         1199 g         1104 t
ORIGIN      
        1 aatgtagcgt gacatcgggt ttaccgccgg cgcctttaaa tttgaggacg atatgcgagg
       61 atggcggaca actgtcgggg aaataccctt gtgtgaactc accggtatac acgcttccga
      121 cactgcactg ggcttcggtg gggtacccca attccaatac ctgaaaagcg ggtgcgatga
      181 tatgacagcc catgtcacca agtgcgcccg tgccataatc ccaccagccg cgccagttga
      241 aagggacaag gttatcgaca tattccctgt aaggcgccgt tcccagccag agattccaat
      301 ccagctcagc cggtaccgtc gccttcgccg tcggccactt gatgccctgc ggccatacgg
      361 gcctgttggt aaaacagtat acttcggtca catccccgat gatccccgcc cggtaccaat
      421 cgaccagttg acggaccccg tcaccagagg caccctgatt ccccatctgc gtgaccacct
      481 tatatctcgg cgccgcctcg gtcagcatac ggacttcata aatgtcatgc gccagcggtt
      541 tctgaatata gacgtgcttc cccaattgca tcgcagccat tccgatgacc gcatgcatgt
      601 gatcgggcgt tgccacattg acggcatcga tgtgcttgtg ctctttttcg agcatctgcc
      661 ggtagtcttt gtaatatttg gcggaaggaa atttcttacg catgttggcc gaccgtctgt
      721 cgtctacgtc gcaaaggacg gcgatatcga ctttacccgt cgccgaaagc caggagaggt
      781 cggattcacc cttaccgccg gctcctaccc cggcgacaac caaccgatcg cttggtgcgc
      841 gataaccttt tccgccgagg acatgacgtg ggacgatata aaaccctgcc gcggctacca
      901 atcctttttt cagaaattcg cggcgggatc cgttttgtac gggactgtat tttttagaca
      961 tagcaagaaa cccccttttt tgaacaattg tcgatagggc ttcttaaaag taaggatttc
     1021 aattattttt cgagggaata gcaaggaatt ttttgcccga caggacctgt ctgacggccc
     1081 ataccagctc gtcggcggcg gcatccctgc ccaatatgcc ggtgattcca gttcgcaatg
     1141 cgcgatatcc atgatgttca tgggtataaa gattcagaag caggaccggg cggtccgggt
     1201 cttccggcat ccggctgccg atcccgtggt gatcggaaat gatcacatcc caggggcgat
     1261 ccatcactct ttccgtccat tcctcccctt ctcctatctc ttcgatctgg gcctgcggaa
     1321 gggcctcggt caatatctgt cgcattcctt tccggatgat cgtatgatct tcagccaata
     1381 atatcctgac catagggctg ttcattgccg acaagatagc gggattttaa aagagtttgt
     1441 acccgcaaga taagggaaag cacttagatc ggataatttt tgatttttgc ctacgtatat
     1501 ctactcattc actgtcatgg ctttgcaagg ctgacgatct tttactcgct gtcgcggctc
     1561 tgcaacgctg acgatctctt actcgctgtc gcggctctgc agggccgctt cgatcctggc
     1621 cttgtcggca tcggtaaaat gtccccgaag atggaaaaag agccctcccc cgtcctcgcc
     1681 gacgatcaga caggcaattt ccttctggtt tgcctggccg tcgcgtgaca gcagctgaaa
     1741 cctgcttttg cctttccgaa cggagaccta ttcttcaaat ctgtcggccc gtagcgaggc
     1801 cccgaggtcc gaccattcat gtgcccccgt ggtactcttc ttcccgtcga tgatcagaca
     1861 cctgatcgag gtgatcctgt gcgtccagtc tttatcacta tcggcagcat cgcggttaaa
     1921 gctggcgcta aaaaggaacg cggggtcgac ggatagcttg tcggtgcctt cggtcgcctg
     1981 atatttccga taaaattcat ccaggtgcga gggttgggcg caggctgaaa aggcagccag
     2041 gatgatgagc agggggagca tagtcttacg catattattg tctttatgaa tttttaatgt
     2101 cttttcgcat ttttaagatc ttccattcct ttgataccca tgtccgatga cagccttgac
     2161 aacttgtcca ggtcgatgtc tccgacgaaa ctcatcaggg tgaagccatt ttcgtcgacg
     2221 gcgatcatga gcagctcgcg tatcactttc ccatcatcgc gcaccataaa tcgcacattg
     2281 tctttgctgc tgcggagcat gatgaggtct tcgtattgtc gccggtcgat agctgcgagc
     2341 gcctccttgt aaaagaccat cggggtcgtt tcggtgctga tgatccggaa gctttgcagc
     2401 cgtgtaacgg tctgctgaag atcggcgggg atcgtgtccc atttcacctt cgagagtagg
     2461 cggaacatct tcgggctgac gctcacgacg gtaaagcggg agtcgtcgag atattttccg
     2521 aagaatcttc cgatggcatc gtcctggcaa aaggctcccg tgcatagcag caacaggggc
     2581 aggaccaaca gatgacgttt aacttttcgc atgatttaat ttttgaggcc ggtgatcgac
     2641 cggcggcttt cattcaaatg ggtagaagcg atcagcaagg catgacggac ggctgcgagc
     2701 gcttgttccg ggttatcata ggtgtccgtg atcgtcgtcg cgacaacaac cggcggggcc
     2761 tgccgcacag caggcgtaac cagaaaaagc cctgcgacga tcgcacagat cgcggcggcg
     2821 gccgcgataa atcccaaacg gaaggtcctt acggacgagg cgggaccgtg gagaccctga
     2881 gaggcgtgaa gacccggaga gccggataga ccgacatgct gcagtatgcg gttttcgaaa
     2941 ccgggcccgg caatcacttc tgcctcctga ccgaaataga ggaacaagtc acgatagggc
     3001 gccagatcgg ggtcgatgtc attctgcccg cgaaaatatt cagcgaggac cttttcctct
     3061 gcgacggtcg tcccggcctc gaagtatttt tctaaaagcg cgattacggt tgccttttcc
     3121 atatttcctc ctctttgata atggtctttc tgatggcgct acgtccccgg aacaggttga
     3181 cctttacctg gtccagcgtc atgtccagca cttcggcgat ctcgttatag ctgaacccct
     3241 ccatttcccg gagacggatc acgtgctgtt gttgcggcgg caaatcgtct atgcattcct
     3301 gtatacgtcg catcatctct ttattggtta ccttctcgta cgggtcgcga tcggggctgc
     3361 tgatctccgc cgcggcgcct tcgcgtatag tggttgtcgt tcgccgccgc aatctgtcga
     3421 gacagttgtt tcgggtagcc gtcatacaat atgccggtat gcttttccac tgcaaccatt
     3481 ccgccctccg ggcccaaact cccgccatta cgtcctggac ggcgtcctct gcctcctgca
     3541 catcctgcag aaaacgatag gcaaaccgga agagcttgtc tttcatcggc aaaatgtgct
     3601 gtttgaaggc ttcttctgac attactgtga aaatgacggc aaaaaatgac taatgttaca
     3661 ggaaattaac tgccttgaaa aaatattatt cggtcgccgg gcgggtgtct ccggtcgcgg
     3721 ctttggtgct cgctatcgcc gggcgggtgc tcccgatcct ggttttcata ctgacggccg
     3781 ggtcgctgca tgcgcaggac cctatcggtc tgtccgatct gcggtccgcg gaaaaattat
     3841 tcgggctgca tttcaccccc gtaaaggagg attccatact gagcggtctg gacgaccatc
     3901 ttcgtcttta tcaatacctg catgagcacg atctgtacaa cgacgtgcct ttgtcgctgt
     3961 cgtttgatcc gctgctgccg ggtacgccgt acgatcgcaa acaggcgccg ctgcaatggg
     4021 atctcccgca gaatgttacg cttccggcca accggaatga ccttgcctgg tattccattc
     4081 cagagctggc ctcgctcctc cggcagaaaa agatcagctc ggtagacctg acgacctttt
     4141 tcctggaccg gctcaaacgt tacgggcccg ttctgcactg cgtgatccag ctgctggaag
     4201 acagtgcgct ggcccaggcg aagagggcgg acgcagatct ggccaaaggc gtgtaccggg
     4261 gtcctttgca gggcattccc tatggcatca aggatctttt tgcggtaaaa ggcagctata
     4321 cgacatgggg ttcgccgcct tataaggggc agaccataga gcaagactgt tatgtggcta
     4381 aaagactcgc ggaggccggc gcagtgctgg tggcgaaatt gtcgctcgga gagttggcga
     4441 tggacgatgt ctggttcgga ggtcttaccc gcaatccctg gaaaccgcag acgggttc
//
LOCUS       DASYSV010000546         3893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2033392, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000546 DASYSV010000000
VERSION     DASYSV010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033392"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VGQ51_17010"
     CDS             <1..450
                     /locus_tag="VGQ51_17010"
                     /inference="COORDINATES: protein motif:HMM:NF024597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 66 protein"
                     /protein_id="HEV9038340.1"
                     /translation="KPDLQQAMVHYYDFLTGYEDLLQDGGTLDQPDLVSADGKVVLSN
                     WSPQPGAVSMVGKDMGGREVIQLINLANATTFDWRDANANQRVPNTILNAAFIFTARR
                     PVKKLWYATPDADGGVAKQLSFSSSGNTISFALPSLQYWDMVVAEYQ"
     gene            461..3148
                     /locus_tag="VGQ51_17015"
     CDS             461..3148
                     /locus_tag="VGQ51_17015"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEV9038341.1"
                     /translation="MKQRSIFIGLFLLTACQQASAQDSRAIFSAGRFRLYADSVTQYP
                     FAGVAVSDSELRSDYRSPGNPYRSSRIAFKFSINGRDNEMKSGNDDHFNCTGLTNETP
                     VIRFGESFQDTTTVYPPYLQPGATLKVRLDMRKVLTGFDSGGYYTLFNGQRLYKKDFK
                     GVYIAGDAPPLTWDFDHLADHPDLRLQDVDGDGIFETTLALNKANDSGRKVSTWKLNK
                     DISAFPQYHSGYPISDAVYNLALEEMIKAVESDSTFRTGKLWSGVWTRDISYSIILSM
                     AYLQPRVAMNSLMRKVNAHKKIIQDTGTGGAYPVSSDRMIWAVAAWEVYETTGDEEWL
                     QQAYRIIRNSVEDDLLNSYDRATGLVRGESSFLDWREQTYPAWMQPADIYASECLGTN
                     AVHYQANIVLARMALLLHHPEAAQKYRSIAMRIKAGINRYLWQEDKGFYAQYRYGRDY
                     KIVSGRAEGLGEALCILFDIADRKKRQRIVSRMPVTDFGIPCIYPQIPGIPPYHNNAV
                     WPFVESFWAQASARADNEQSVMEAIAMVYRPAALFLTNKENFTAENGDYASTQINSDN
                     MLWSLSGNISLVHKILFGIRFQPDSLLFRPFVPRELRGRRSLTNFTYRGAVLDITEEG
                     YGDKVDAFYLDGRKLLSAAIPARLTGHHTVRIVIAGHRFPALGVRRVRNYTSPATPAV
                     IYRAGYLRWDPVAGAVGYRVLRNGMIFSELPETRLAISDSVYVEWQVVAVDPAGVASF
                     ASEPVTTGGCVRRYEIEDFASPSALPYKGFSGKGFVETGRTINQQLTMAVEITEPGVY
                     ALDFRYANGNGPINTSNKCAIRTLREKGSFLGTIVLPQRGEDWNKWGFSNAVRVYLSK
                     GTHYLRLAYEPQDQNMNGDINGAMLDYLRVVRLSGPCLR"
     gene            complement(3164..>3893)
                     /locus_tag="VGQ51_17020"
     CDS             complement(3164..>3893)
                     /locus_tag="VGQ51_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038342.1"
                     /translation="YNDKNELIRTLQWNVDSGFNRDYWGMEEKGFRQPGTPKLPSGAQ
                     EPGGLQVLPGTYKVVITYARASDSTFVTVKDDPRFGNRNEIKLAQRALYDRLKKSVDK
                     LTEGMDRLTESEEVCAKLQTELRGLEGKDVDSVRKMTTKMQDSIKAIRELINGKTSDK
                     QGLARNPFDVTVMSQVREAGQSIGSKMTSPGAQEETMVANAEKAIAEAVQKINTFFDG
                     RWKDYRALVEATKVSLFKDYKPIQ"
BASE COUNT          836 a          966 c         1095 g          996 t
ORIGIN      
        1 aagccggatc tgcaacaggc tatggttcat tattacgatt ttctaacggg ttatgaggat
       61 cttttgcagg atggtgggac attggatcag ccggatcttg tctccgcaga tgggaaagtt
      121 gtgttaagta actggtcgcc ccagcccggc gccgtttcga tggtgggcaa ggacatgggc
      181 ggcagggaag taatccagct gatcaacctg gccaatgcga ccacattcga ttggagggac
      241 gccaatgcca accagcgtgt gccgaatact atcctcaatg cggcctttat tttcacggcc
      301 cgcaggccgg taaagaagtt gtggtatgcc acgccggatg cagatggcgg ggttgccaaa
      361 cagctctcct tttccagctc agggaatact atttcttttg cgttaccgtc tcttcagtat
      421 tgggatatgg tggtagcgga atatcaataa acctcatccg atgaaacagc gatcgatctt
      481 tatcggcttg ttcctgctga cggcctgcca gcaggcgagt gcacaggaca gccgggcaat
      541 cttctccgcg gggcgttttc ggctctatgc cgatagcgtt acgcaatatc cttttgccgg
      601 cgttgccgta tcggattccg aattgagatc ggactaccgc agtcccggca atccctaccg
      661 atcctcaaga atagccttca agttcagcat caacgggcgc gacaatgaaa tgaagtccgg
      721 gaacgacgat cactttaatt gcaccgggtt gacgaatgaa acgcccgtga tccgttttgg
      781 cgagtctttc caggacacca cgacggttta tcccccgtat ctgcagccgg gggccacatt
      841 aaaagtgcgg ctggacatgc gaaaggtcct gaccgggttc gattccgggg gatactatac
      901 gctattcaat ggccaacggc tttataaaaa ggatttcaaa ggcgtgtata tagccgggga
      961 tgcaccgccg ctgacctggg atttcgatca cctggcggat catccggatc ttcgtttgca
     1021 ggacgtcgac ggcgacggca tcttcgaaac gacattggca ctgaataaag cgaatgacag
     1081 cggcagaaag gtttccacct ggaagctgaa taaggatatc tccgcctttc ctcaatatca
     1141 ttccggctac ccgatctccg atgccgtgta taacctggcg ctcgaagaaa tgatcaaggc
     1201 ggtggagtcg gacagtacgt ttcggacggg gaagttgtgg tccggggtat ggacgcggga
     1261 tattagttac agcatcatat tgagcatggc ctatctgcaa ccgcgggtag ccatgaatag
     1321 cctgatgagg aaagtaaatg cgcacaaaaa aattatccag gatacgggta ccggcggcgc
     1381 ctatccggtt tcttccgacc ggatgatctg ggcagtggcc gcctgggaag tatatgaaac
     1441 gacgggggac gaagaatggc tgcagcaagc ctataggatc atcaggaatt ccgtcgaaga
     1501 cgacctgttg aatagctatg accgcgcaac cggtctggtg agaggggagt cgtctttttt
     1561 ggattggcgg gaacagacct atcccgcctg gatgcaaccg gcggatattt atgcttcgga
     1621 atgcctgggt accaacgctg tccattacca ggcgaatatc gttctcgcgc ggatggcttt
     1681 gttgttgcat cacccggaag cggcgcaaaa gtacagatcc attgccatga ggataaaggc
     1741 gggtatcaat cgctatttgt ggcaggaaga caaggggttc tatgcccaat accggtatgg
     1801 acgggattac aagatcgtat cgggccgggc cgaggggctc ggtgaggcgc tgtgtatcct
     1861 atttgatata gccgaccgaa agaaacggca gaggatcgtt tcccggatgc ccgttaccga
     1921 cttcggcatc ccatgcatct acccgcagat accgggcatt cccccctatc ataataatgc
     1981 cgtctggccc tttgtggaat cgttttgggc gcaggcttca gcgcgggcgg ataatgagca
     2041 gtccgtaatg gaggctattg ccatggtata tcgtcccgct gcgctttttc tgacgaataa
     2101 ggagaatttt acggctgaaa atggcgacta tgcatccacg cagatcaatt ccgacaatat
     2161 gttgtggagc ctatcgggga atatcagtct tgtccataag atcctttttg gtattcggtt
     2221 tcaacccgac tcgctgcttt ttcgtccttt tgtcccacgg gagctgcggg gccggcgttc
     2281 gctcacgaat tttacctata ggggtgctgt gctggacatc acggaggaag gttatggcga
     2341 caaggtggat gcgttttacc tggatggccg aaagctatta tcggccgcca ttccggccag
     2401 gttgaccggt catcatacgg tgaggatcgt cattgcgggg catcgatttc ccgcgttggg
     2461 cgtcaggcgt gtacgcaatt atacttcgcc tgccactccc gcggttattt atcgggcggg
     2521 atatttgcga tgggatcccg ttgcgggcgc ggtcggttac cgcgtattga gaaatgggat
     2581 gatcttttcc gagcttcccg aaacccggtt ggccatttcg gacagtgttt atgtggaatg
     2641 gcaggtggta gcggtggatc cggcgggagt cgcctctttt gccagtgagc cggtgacgac
     2701 aggcggctgc gttcggcggt atgagatcga ggattttgca tccccgtccg cactgcctta
     2761 taaggggttt tcgggtaaag gttttgtaga gaccggcagg acgatcaacc aacagttgac
     2821 gatggcggtg gagatcacgg agccaggtgt ctatgcgctc gacttccggt atgccaacgg
     2881 gaatggtccc attaatacca gtaataaatg cgctatccgt acgctgcgcg aaaaaggttc
     2941 ctttttgggg actatcgttt tgccccaacg cggggaggat tggaacaagt gggggttttc
     3001 caatgccgtc cgggtatatt tatcgaaggg cacgcattat ttgcgattgg cgtatgagcc
     3061 gcaagatcag aatatgaacg gcgatatcaa cggggcaatg ttggattacc tgcgggtagt
     3121 tcggctgagc ggtccatgtc ttcgataggg agtgggtacc gggttactgt atcggcttat
     3181 aatccttaaa caggctcact tttgtcgctt ccaccagggc gcggtaatct ttccatctcc
     3241 catcgaaaaa tgtattgatc ttctgaacag cttccgcaat cgctttctcc gcattcgcga
     3301 ccatcgtctc ttcctgagca cccggcgatg tcattttact tccgatggat tgcccggctt
     3361 cgcgtacctg ggacatcacc gttacatcga acgggtttcg tgccagacct tgtttgtcgg
     3421 aggttttccc attgatgagt tcgcggatcg cttttatact atcctgcatt ttagtggtca
     3481 tcttccttac ggaatcgacg tcttttccct ccaggccgcg tagttcggtt tgaagcttgg
     3541 cgcaaacttc ctccgattcg gtcagcctgt ccatcccctc ggttaattta tcgacggatt
     3601 ttttcaatcg atcatacagg gcccgttgcg ccagcttgat ctcatttcta ttgccgaacc
     3661 ttgggtcgtc ttttaccgtt acaaaagtcg aatcggatgc cctggcatat gtgatcacta
     3721 ctttataggt gccgggcagg acctgtaagc caccaggctc ttgtgcgccg gatggcagtt
     3781 ttggcgttcc tggttggcgg aagccttttt cttccattcc ccaataatcc cggttgaagc
     3841 cgctatcgac attccattgc agggttcgta tcaactcgtt cttatcgttg taa
//
LOCUS       DASYSV010000547         6830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1518989, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000547 DASYSV010000000
VERSION     DASYSV010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518989"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VGQ51_17025"
     CDS             complement(<1..308)
                     /locus_tag="VGQ51_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEV9038343.1"
                     /translation="MSMRLKTKLSLGLIFLFIVIVLFGVLGLFFINRLSDEGRLVLQN
                     NHESLVYCNRMLQALESLPGDKNALSQFRDNLRKQQTNVTEVGEKEATDELTRNFNEL
                     L"
     gene            complement(324..1478)
                     /locus_tag="VGQ51_17030"
     CDS             complement(324..1478)
                     /locus_tag="VGQ51_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein KdpD"
                     /protein_id="HEV9038344.1"
                     /translation="MADTEKEIKPAADKAPDVQHFLDLIRQSRRGKFKIYIGMSAGVG
                     KTFRMLQEAQTLLRNGVDVKIGYVETHGRPETEALLQGLPVMPRRKLFYKGKELEELD
                     VQGVINLHPEVVVIDELAHTNIEGSKNEKRWQDVMDVLDAGINVISAVNIQHIESLYE
                     EIRDITGIEVTERIPDSVLEEADEVVNIDLTADELVTRLKEGKIYAADKIGIALQNFF
                     QPEKILQLRELALKEVASQVERKIETELPRGAHLPNERFLACISSNHETAKKVIRKTA
                     RLASYYHSRWWLLYVQTTKEAGDRIGLAAQRHLINNFKLATELGAEVIRIQGNNIAKG
                     IMDIAESRSITTICIGKPHLSLYNVIMSTAVFNQLLNKLAASDIDIVILS"
     gene            complement(1465..2028)
                     /locus_tag="VGQ51_17035"
     CDS             complement(1465..2028)
                     /locus_tag="VGQ51_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEV9038345.1"
                     /translation="MKRYILPALKLTIVMIVICGLLYPLSIAAVGKLTRGGGNGETVS
                     LHGKVVGYANIGQKFTEDKYFWSRPSAVGYNAAGSGGSNKGPSNPDYLKDVQAHIDTF
                     LAHNPGVRKEDIPAELVTYSGSGLDPDLSPAGAKVQVGRVARARGLSDKELNDLIDRY
                     TDGPFLGIFGPAKVNVLKLNIALDGGH"
     gene            complement(2040..4052)
                     /gene="kdpB"
                     /locus_tag="VGQ51_17040"
     CDS             complement(2040..4052)
                     /gene="kdpB"
                     /locus_tag="VGQ51_17040"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEV9038346.1"
                     /translation="MKPFVLQALKQSFVKLSPATMYRNPVMFAVYIGTWIMGIVCIWI
                     LAGEKRQGHFGYNLTVFLVLLVTVLFANFAEAIAEARGKAQANSLRKTREDTPAKKIF
                     AVGEIYVGEIKIVSSSHLQKGDVFIAEAGDTIPMDGEIIEGLATIDESAITGESAPVI
                     REAGGDKSSVTGGTKVLSDKIKVRVTTSPGESFLDKMIALVEGASRQKTPNEIALTIL
                     LAGFTLIFLIVVVTLKPFADYANTPITVAAFVSLFVCLIPTTIGGLLSAIGIAGMDRA
                     LRANVITKSGKAVETAGDLDTLLLDKTGTITIGNRKATHFWPAPGVDDKAFMEACVLA
                     SLADETPEGKSILELAGANPTSYNTTGARFIGFSAETRSSGIDVSGMHIRKGAFDAIR
                     AIVTRAGNVVPDKTEAKVKEIASNGGTPLAVSENGRAMGVIELQDIIKPGIQERFQRL
                     RKMGVKTVMVTGDNPLTAKYIAEKAGVDDFIAEARPEDKMNYIRKEQQSGKLVAMMGD
                     GTNDAPALAQADVGVAMNSGTQAAKEAGNMVDLDNDPTKLIEIVEIGKQLLMTRGTLT
                     TFSIANDVAKYFAIVPALFITSIPALQAMNIMQLKSPESAILSAVIFNAIIIPVLIPL
                     ALRGVAYKPIGASALLRRNLLIYGVGGIVAPFIGIKAIDLLLGSIM"
     gene            complement(4059..5774)
                     /gene="kdpA"
                     /locus_tag="VGQ51_17045"
     CDS             complement(4059..5774)
                     /gene="kdpA"
                     /locus_tag="VGQ51_17045"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEV9038347.1"
                     /translation="MGTELFGVVVSFLITVLLAVPLGKYIARVYRGDRTILDFMKPVE
                     RLIFRFCGIDPNRKMNWKEFLKAMLTINLVWFIYAFILFLFQGKLPLNPDGNPSMTPD
                     LTFNTAVSFLVNCNLQDYSGETGATYLTQLFVITFLQFVSASTGIACLVALFNGLKEK
                     TTQNLGNFWDIFTKTMTRILLPLCIIIAVLLAMHGTPTSYAGKDTVITMQGDTVQVSR
                     GPAAGMIAIKHLGTNGGGWFGANSAHPLENPDYFTNMLEIIAQALIPIAMIFSLGFYI
                     NRKRFAYVTFAIMTVGFLCLVIPTMISEIHGNPAIARMGITQATGAMEGKEVRLGPAA
                     SGYWSILTTVISTGSVNSMHDSSMALSGLMQLLAMMVNAFYGGCGVGILNYYIYVIIA
                     VFIAGLMVGRTPELFGHKVEAREVKIAALVTLLSAFLIKGFTAFACYILVYHGNAAWA
                     VKPGAWLNNPGYHGFSEMLYQFTSTNANNGSGFEGLADNNVFWNVTGGIVMILGRYLP
                     IIGPVAIAGLLANKKYVPESSGTLKVDSLTFGIMIFIVIVIITALSYFPALALGPIAE
                     YFTLR"
     gene            complement(6015..>6830)
                     /locus_tag="VGQ51_17050"
     CDS             complement(6015..>6830)
                     /locus_tag="VGQ51_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV9038348.1"
                     /translation="FAQAIHFASKRSSKPFVPLNCSAFSKELLESELFGHKAGSFTSA
                     TRDKKGLIEEANQGTLFLDEIGEIHIDLQAKLLRVLETNEFIKVGDTRPTRVNVRIIA
                     ATNRDLEREVADGQFRDDLFYRLNVFSISLPSLRERKKDIPAIAEFYLRWFAGKANKH
                     IQSMSKEFLERLQQHEWKGNIRELKNIIERAVILSDGGELTPENLPADLQVAGLRHTG
                     PISAFDLASVEKLHIQRVLNHTRGNKTEAARLLNIGLTTLYRKIEEYKLSYEA"
BASE COUNT         1500 a         2007 c         1703 g         1620 t
ORIGIN      
        1 agcagctcgt tgaaatttcg cgtcagctcg tccgtcgcct ccttctcacc tacctccgtt
       61 acattggttt gctgtttcct caggttgtcc cggaactggc ttaaagcatt cttgtccccc
      121 ggaaggctct ccaaagcctg cagcatccgg ttgcagtaaa ccagcgattc atgattgttc
      181 tgcaacacca gcctgccttc atcgctgagc cggttgatga aaaagagacc tagtacgccg
      241 aaaagcacga tgacgatgaa caggaagatc agacccagcg ataatttggt tttcaaacgc
      301 atgctcatgc tcctaaaatt agttcacgaa aggatcacaa tatcgatatc cgatgctgcg
      361 agtttattca gcagctggtt aaataccgcc gtgctcatga tcacattgta caggctgaga
      421 tgcggtttcc cgatacagat ggttgtaata gaacgactct cagcaatatc cattattcct
      481 ttggcgatat tgttcccctg gatccggatg acttccgcgc ccagctcggt cgccagcttg
      541 aaattgttga tcaggtgacg ttgcgccgcc aacccgatcc tgtcccctgc ctctttcgtc
      601 gtctgcacgt acaacagcca ccaccggcta tgataatagc tcgccagacg ggcggttttc
      661 cgaatgacct ttttcgccgt ttcgtgatta ctgctgatgc aggccaggaa acgttcgttg
      721 ggcaaatgcg cgccgcgcgg cagctccgtc tctatcttgc gttcgacctg gctggcaacc
      781 tccttcaacg ccagctcccg caattgaagt atcttctccg gctgaaagaa attctgcagc
      841 gcgatcccga tcttgtccgc cgcgtagatc ttaccttctt tcaatcgtgt caccaattcg
      901 tccgcggtca ggtcgatatt gaccacttcg tccgcctctt ccagcacact gtccgggatc
      961 cgctcggtga cctcgatccc cgtaatatcc ctgatctcct cataaagact ctcgatatgc
     1021 tgtatattga ccgcgctgat gacattgatc cccgcatcca ggacgtccat cacatcctgc
     1081 cagcgctttt cattcttgct gccctctata ttagtatgcg ccagctcgtc gatgaccacc
     1141 acttccggat gcagattgat gacgccctgt acgtccagct cttccagctc cttgccttta
     1201 tagaacaatt tgcgccttgg catgaccggc agtccctgca ataaagcttc ggtctcgggc
     1261 cgcccatgcg tctccacata accgatcttt acgtctactc cattccgcaa caaggtctgc
     1321 gcttcctgca acatgcgaaa ggttttccct accccagcgc tcatgccgat gtatatcttg
     1381 aatttgccac gacgggattg ccggatcagg tccagaaaat gctgaacgtc gggagccttg
     1441 tccgcagcag gtttaatttc tttttcagtg tccgccatca agcgcaatat tcaatttgag
     1501 gacattcact ttcgcaggcc caaagatacc aaggaaaggg ccatccgtgt atcggtctat
     1561 caggtcattt aattctttgt ccgataatcc ccgggcccgc gctacccttc cgacctgcac
     1621 tttcgcgcct gccggcgaaa gatcgggatc caacccgctg ccggaatacg tcaccagctc
     1681 ggcggggata tcctccttcc tcaccccggg gttatgcgcc aaaaaggtat cgatatgcgc
     1741 ctgcacatcc ttcagataat cgggattcga aggccccttg ttgctgcctc cgctgccggc
     1801 ggcattatac cccacagcag acggccggct ccagaaatac ttgtcttcag tgaacttctg
     1861 cccgatattc gcatagccca ctactttgcc atgaagcgat accgtttcgc cattcccccc
     1921 gccccgggtc agctttccca cggccgcgat cgataaggga tagagcagac cgcagatcac
     1981 gatcatcacg atcgttagtt taagagccgg taatatatat cttttcattt tcttcgtttt
     2041 tacatgattg atcctaatag caggtcgata gccttgatcc cgataaaagg cgcaacaata
     2101 ccacctacgc catagatcaa aagattccgc cgcagcaacg cactggcgcc gataggcttg
     2161 taggccacac cgcgaagcgc cagcggtata aggaccggaa taatgatcgc gttgaagatc
     2221 accgccgaaa ggatggcact ttcggggctt ttgagctgca tgatgttcat ggcctgcaat
     2281 gccgggatgg acgtgatgaa caacgccgga acgatggcga aatatttcgc tacgtcattc
     2341 gctatggaaa aggtcgtcag cgtccctcgc gtcatcagca gctgtttgcc gatctccacg
     2401 atctcgatca gcttggtcgg atcgttatcg agatccacca tattgcccgc ttcctttgcg
     2461 gcctgcgtgc cgctgttcat ggctactcca acatccgcct gcgccagcgc cggcgcgtca
     2521 ttggtgccgt cgcccatcat cgctaccagc tttccgctct gctgctcctt acggatatag
     2581 ttcatcttgt cttccggtct ggcctcggcg ataaaatcgt ccacacccgc cttctcggcg
     2641 atatattttg cggtcagcgg attatcgccc gtcaccatca ccgtcttaac gcccatcttg
     2701 cgcagacgct ggaacctttc ctggatgccc ggcttgatga tatcctgcag ctcgatgact
     2761 cccatcgccc gtccattctc gctcacagcc aacggggtac cgccgttcga tgcgatctct
     2821 ttcaccttcg cctccgtttt gtcgggaacc acgttccccg cccgcgtcac gatggcgcgg
     2881 atagcgtcaa aggcgccttt acggatatgc atcccggata catcgatacc gctgctccgc
     2941 gtttccgccg agaacccgat gaaccgcgct ccggtggtgt tataggaagt cggattggcc
     3001 cccgccagct ccaggatcga tttgccttcg ggtgtctcat cggcaagcga cgccagtacg
     3061 caggcttcca taaaggcttt atcatcgacg cccggcgcgg gccagaaatg cgtcgccttg
     3121 cggttcccga tggtaatggt ccccgttttg tccaataata aggtgtccag gtctcccgcc
     3181 gtctcaacgg cttttccgct cttggtgatg acattggccc ggagcgccct gtccataccg
     3241 gcaataccga tggcgcttag caacccgcca atcgtggtcg gtatcaggca aacgaacagc
     3301 gatacgaatg ccgccaccgt gatcggcgta ttggcatagt cggcaaatgg cttcagcgtg
     3361 acgacgacga tcagaaagat cagggtgaac ccggccagca ggatcgttag ggcgatctcg
     3421 ttcggcgttt tttgccgtga tgcgccttca accagtgcga tcatcttgtc gaggaaactc
     3481 tcccccggcg acgtcgtcac ccgaaccttg atcttgtcac tcagcacctt tgtgccgccg
     3541 gtgaccgacg atttgtcgcc gccagcctcc cggatgacgg gtgccgattc accggtaatg
     3601 gcgctttcat cgatggtcgc cagaccctct atgatctcac cgtccatagg gatcgtatct
     3661 cccgcttcgg cgatgaatac atctcctttc tgcagatgcg aggaggatac gatctttatt
     3721 tctcccacat agatctcgcc cacggcgaag atctttttcg ccggcgtgtc ctcccgcgtt
     3781 ttgcgcagac tgttggcctg cgccttgccc ctcgcctccg cgatcgcctc tgcgaaattg
     3841 gcaaaaagga cggtcaccag caataccagg aagaccgtca gattatagcc gaaatggcct
     3901 tgcctctttt ctccggccag tatccagata caaacaatgc ccatgatcca cgtcccgata
     3961 tatacagcaa acatgacagg gttccggtac atggtcgccg gcgataattt gacgaaggat
     4021 tgtttcagcg cttgcagtac gaatggtttc ataacttttt atcttaaagt gaaatattct
     4081 gctatcgggc ccagtgccag ggccgggaag tacgacaaag ccgtgataat tacgatcact
     4141 ataaatatca ttatcccgaa ggtcaacgaa tccactttca atgtccccga cgattccggc
     4201 acatattttt tatttgccag caatcccgcg atagccaccg gaccgatgat cggcagataa
     4261 cgtcccagga tcatgacgat accgccggtt acattccaga acacgttatt gtccgccagt
     4321 ccttcgaaac cgctgccatt attggcgttg gtggaagtaa actggtaaag catctccgag
     4381 aatccatgat accccggatt gttcagccac gcgcccggct tcaccgccca tgcggcattt
     4441 ccatgataga cgaggatata acaggcaaat gctgtgaaac ccttgatgag aaaagcagaa
     4501 agcagcgtca ccaacgcagc gatcttcacc tcccgggcct ccaccttatg cccgaagagt
     4561 tccggcgttc gaccgaccat gagccccgca ataaacacgg cgatgatgac atagatatag
     4621 tagttgagga tccctactcc gcaaccgcca taaaaggcgt tgaccatcat ggccagcaat
     4681 tgcatcaacc ccgaaagggc catcgagctg tcgtgcatcg aattcaccga acccgtcgag
     4741 atgacggtcg tcaggatact ccagtagccc gaggctgcgg gtccgagacg tacctccttg
     4801 ccttccatcg cgccggtagc ctgcgtgata cccatccgcg ctatcgccgg gttgccatgg
     4861 atctcgctga tcatagtcgg tattaccagg caaaggaatc cgacggtcat gatcgcaaac
     4921 gtcacatagg cgaacctttt ccggttgatg taaaacccaa gggagaagat catcgctatc
     4981 gggatcagcg cctgtgcgat gatctccagc atattggtga aataatccgg attctccagc
     5041 ggatgagccg agttcgcgcc gaaccagccg cctccattcg tcccaaggtg tttgatggcg
     5101 atcatacctg ctgccggtcc ccgcgacacc tgtacggtat cgccctgcat cgtaatgacc
     5161 gtatccttgc cggcatagct cgtcggcgta ccatgcatgg ccagcagcac cgcgatgatg
     5221 atgcataggg gcaacaggat gcgggtcatg gtctttgtaa agatgtccca gaagttgcca
     5281 aggttttgag tggtcttttc cttcagcccg ttgaacaacg caacaagaca cgcaatgccg
     5341 gtcgacgcac tcacaaactg caggaaggtt atcacgaaca gttgcgtcag ataagtagcc
     5401 cccgtctctc cggaataatc ctggaggttg cagttgacca ggaagctgac agccgtgttg
     5461 aaggtcaggt ccggcgtcat gctggggttc ccgtcggggt tcagcggcag cttgccctgg
     5521 aaaaggaaga ggatgaaagc ataaatgaac cataccaggt tgatggtcag cattgccttc
     5581 aggaattcct tccagttcat cttccggttg ggatcgatgc cgcaaaagcg gaagatcagc
     5641 cgttccaccg gcttcataaa atcgaggatg gtccggtctc ctctgtaaac gcgggcgata
     5701 tattttccca gcgggacggc gagcaacacc gttatcagga aggagaccac tacgccgaaa
     5761 agttcggtac ccatacggtt aaaatttctc gggttttagt aatacataga gcaggtagac
     5821 aaagaccgct acggaaacga taaagattgc cgtcatcatc attttttttc agatgggagg
     5881 ccaaaaggga tgcctgaaaa cccgcaatcg ctccgaaggc gcaccaggcc agcattacag
     5941 cctgtcggca ccctctgccg aaggcagatc ccccctacca aaatggaagg catttcgcca
     6001 aaatgggggt gcccctatgc ctcgtaactt agcttatact cttcgatctt gcggtaaagg
     6061 gtggtcaacc cgatattgag gagacgcgcc gcttcagtct tattgccgcg ggtgtgattg
     6121 aggacgcgct ggatatgcaa tttctccaca ctggcgaggt caaaggcgga gataggaccg
     6181 gtatgccgca gcccggctac ctgcagatcc gccggcaggt tctccggcgt gagttcgccg
     6241 ccatcggaga ggatgacggc ccgttcgatg atatttttca attcgcggat attccccttc
     6301 cattcgtgtt gttgaagacg ttcgaggaat tccttgctca tgctctggat atgtttattg
     6361 gccttgccgg caaaccagcg caggtagaac tccgcgatcg cggggatatc cttcttccgt
     6421 tcgcgcagcg aaggcagcga gatggagaaa acgttcagcc gatagaaaag atcgtcgcgg
     6481 aactgaccgt ccgccacctc ccgttccaga tcgcggttgg tcgcggcgat aatgcggaca
     6541 ttcacccggg tcgggcgtgt atcgccgact ttgatgaatt catttgtctc caggacgcgt
     6601 agcagttttg cctgcaggtc gatatgaatt tcgccgatct catcgaggaa aagagtgccc
     6661 tggttggcct cttcaattag gcctttctta tcgcgtgtag cactggtgaa agaccccgcc
     6721 ttatgaccga agagctcgct ttcgaggagc tccttgctga acgcgctgca gttcagcggc
     6781 acgaaaggtt tcgacgagcg tttgctggcg aaatggatgg cctgcgcgaa
//
LOCUS       DASYSV010000548         5713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_622266, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000548 DASYSV010000000
VERSION     DASYSV010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622266"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGQ51_17055"
     CDS             complement(<1..218)
                     /locus_tag="VGQ51_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV9038349.1"
                     /translation="MKHPDIFDWIRVDEYAATPKYLQLTNSILKAIETGKIRKNDLMP
                     SINELSYELEISRDTAEKGYKYLKKMGVL"
     gene            complement(240..836)
                     /locus_tag="VGQ51_17060"
     CDS             complement(240..836)
                     /locus_tag="VGQ51_17060"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEV9038350.1"
                     /translation="MSSRDKILATVTKNQPEGRDLPALPAASAAGGDHAAVFMSTLEA
                     IGGAVYVVSGFDRIATILKEKFPDARRIINGVTETPADIGDPHTLENVDLAILPAHFG
                     VAENGACWITEKQMVQRALPFITQHLVLVLPKSAIVADMHAAYERIDAGDPYGFATFI
                     AGPSKTADIEQSLVLGAHGPASLTVFLLDGQQSDAGYY"
     gene            complement(820..2202)
                     /locus_tag="VGQ51_17065"
     CDS             complement(820..2202)
                     /locus_tag="VGQ51_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEV9038351.1"
                     /translation="MTQHKDHAALAAAFNKDEPRVDWHDQTLWWVRRKRDIAASKVPD
                     WEALRETASGIKDNVLSNLSQYLARFEQESIANGVKVHWASDATEHNEIVYSILHRHG
                     IEKMVKSKSMLTEECHLNEFLDEKGIEVVDTDLGERVVQLAGEPPSHIVLPCIHKKKE
                     EIGEIFHQHLGTPKGNADPKFLTHAAREHLREKFLTRRAALTGINFAVAATGEFVVCT
                     NEGNADMGAHLADVHIACMGIEKLIPERKHLGVFLRLLTRSATGQPITTYSSHFRRPR
                     PGQEMHVVLVDNGRSAHLGRTDFRNSLKCIRCAACFNTCPVYRRSGGHSYHTAVAGPI
                     GSILSPNFDMKKNGDLPFASTLCGSCSNVCPVKIDIHQQLYKWRQVIVQEGYAPLAKK
                     AAMRTMNWVLSKPGVYHTAGKTGRWFMLNVPFTVNNGLNAWYRQREMPEPPGQSFGEW
                     YKKNKHEQQG"
     gene            complement(2189..2914)
                     /locus_tag="VGQ51_17070"
     CDS             complement(2189..2914)
                     /locus_tag="VGQ51_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV9038352.1"
                     /translation="MRVGLFIPCYIDQLYPQVGVATLQLLEKLGCEVVYPPDQTCCGQ
                     PMANSGFEHLTGGCNELFIKNFEDFDYIVAPSGSCVLHIKEHLHGDTGIPKKIFELVE
                     FLTDILKIEHLEATFPHKVGLHQSCHGQRGLHLAQMSELMAAPFSKLERLLSMVRGLE
                     LIGLDRVDECCGFGGTFCVAEEAVSVKMGKDRCADHIKHGAEFITASDMSCLMHLEGI
                     LRRANSNVRVKHIAEILNSNDTA"
     gene            complement(2916..4190)
                     /locus_tag="VGQ51_17075"
     CDS             complement(2916..4190)
                     /locus_tag="VGQ51_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEV9038353.1"
                     /translation="MQNVIAIFDVGKTNKKLFLFDEQYRIVWERSEVCPQTTDEDGDA
                     CEDVVLLTDWVKRSLAEVAALPGLQIKAVNFSAYGASLVHIGFDGTPAAPLYNYLKPY
                     PEDLKKSFYDTYGGEVTFSMLTASPVLGSLNSGMQLYRIKKQRPDLFDSIKVSLHLPQ
                     YLSWLVTGMACSDITSIGCHTNLWNFAQQHYHEWVYREGVIDKLAPIMPSTGVLSLAG
                     VGLHDSSAALIPYLQTFREPFVLISTGTWCITLNPFNDSPLTVAELQKDCLCYMSYQG
                     RPVKASRLFAGYEHEQRVKELAARFHKDPAYAETVRYDADCVDAIRRGRRDDDNFEQA
                     YHLLMTDIMTQQVQSTQLVLHDTGVRRIFVDGGFGRNQVYMHMLADAFPDTEVFAASI
                     PQATAMGAALAIHEHWNSHFPPADIIELKYYR"
     gene            complement(4193..5467)
                     /locus_tag="VGQ51_17080"
     CDS             complement(4193..5467)
                     /locus_tag="VGQ51_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV9038354.1"
                     /translation="MQIEKYKIDEHNHSAEKDHRRKFEFLAGGLKDAEAIIQKLIDFQ
                     VAIPSWALGTGGTRFGRFSGGGEPRSLEEKIEDVGLLHSLNRSSGAISLHIPWDIPRN
                     PASIKALAAQHGLTFDAMNSNTFQDQPGQEQTYKFGSLQNVKKAVRRQAIEHNKEVIK
                     YGVELGSKSLTVWLSDGSCFPGQLNFRQAFQNTLEGLREIYDALPDDWKVFVEYKAYE
                     PNFYSTTVGDWGQSLLYASKLGPKAFTLVDLGHHLPNANIEQIVALLLMEGKLGGFHF
                     NDSKYGDDDLTVGSIKPYQLFLIFNELVEGMDARGMDHARDLGWMIDASHNVKDPLED
                     LLQSVEAILITYAQALLVDRPRLLAAQQANDTVAAQEVLQNSFRSDVRALVAEARLRA
                     GAALDPLALYRIEKVREHLTKERGAGTVATGL"
     gene            complement(5525..>5713)
                     /locus_tag="VGQ51_17085"
     CDS             complement(5525..>5713)
                     /locus_tag="VGQ51_17085"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEV9038355.1"
                     /translation="IFLDETTLTLFAALCIDDAAKLDSLPAQPVMQRWWAYMQDIMET
                     NPDHSPVTRPLNEVFYMP"
BASE COUNT         1233 a         1615 c         1488 g         1377 t
ORIGIN      
        1 aggacgccca tctttttgag atacttatag cctttttcgg ccgtgtcgcg cgagatctcc
       61 agctcatagc tcagctcatt gatcgacggc atcaggtcgt tcttcctgat tttaccggtc
      121 tctatcgcct tgaggatcga gttggtcagc tgcaggtatt tgggcgtggc cgcatactcg
      181 tcgacccgga tccaatcgaa tatatcgggg tgcttcatgc gtgcctaagt tacggtattt
      241 caataatacc ccgcatccga ctgctgacca tccagcagga aaacggtcag actggcaggc
      301 ccatgtgcgc ccagtaccag cgattgctcg atatccgcgg ttttcgacgg ccccgcgata
      361 aaagtggcaa aaccataagg atcacccgca tcgatccgct cgtaggccgc atgcatatcg
      421 gcgacgatag cgcttttcgg cagcaccagc accagatgtt gcgtaataaa aggcaatgcc
      481 cgttggacca tctgtttttc ggtgatccag cacgcgccgt tctccgcgac tccgaaatgt
      541 gcaggcagaa tggccagatc cacattttcg agggtgtgcg ggtccccgat gtccgccggc
      601 gtttcggtta ctccattgat aatccgccgc gcatccggaa acttttcttt caatatcgta
      661 gcgatacggt cgaagcccga gacgacgtac actgccccgc cgatcgcttc gagggtgctc
      721 atgaataccg cggcatgatc ccctcctgct gcggaagcag ccggcaacgc cggcagatca
      781 cgtccttccg gctgattctt cgttacggtc gcgagtatct tatccctgct gctcatgttt
      841 attcttttta taccactctc caaaggattg tcccggcggc tcgggcatct cgcgctgacg
      901 ataccacgca ttcagcccgt tgttcaccgt gaagggaacg ttgagcatga accatcgtcc
      961 cgtctttcct gccgtatggt agacaccggg tttcgacaag acccagttca tggtccgcat
     1021 agctgctttc ttggccagcg gcgcataccc ttcctgtacg atcacctgtc tccacttata
     1081 cagctgctgg tgaatatcga tctttaccgg gcacacattc gagcatgatc cgcacagtgt
     1141 ggaggcaaac ggcagatcgc catttttttt catatcgaag ttaggcgaca ggattgaacc
     1201 gatgggtccc gctaccgcgg tatgatagct gtgtccccca ctgcgccggt agacggggca
     1261 ggtattaaaa caagcggcac accggataca tttcagcgaa ttacggaaat ctgtgcgacc
     1321 cagatgtgcg ctgcgtccat tgtctacaag cacgacatgc atctcctgtc cgggtctggg
     1381 ccttctgaaa tgactcgaat acgtcgtgat cggctgcccc gtggcgcttc gtgtcagaag
     1441 acgcaggaaa actcccagat gtttccgctc cgggatcagc ttctcgatac ccatgcaggc
     1501 gatatgcaca tccgcaaggt gtgcgcccat gtcggcattg ccttcattgg tacaaacgac
     1561 gaattccccc gtcgccgcaa cggcgaaatt gatgccggtg agtgcggccc tccgggtcag
     1621 gaatttctcg cggagatgct cccttgccgc atgcgtcagg aatttcggat cggcattgcc
     1681 tttaggagtg ccaaggtgct gatgaaagat ctctccgatc tcttcctttt tcttatggat
     1741 gcatggcaat acgatgtggc tcggcggttc gccggccagc tggacaacgc gttcccccag
     1801 atcggtgtcc accacttcga tgcccttttc gtccaggaat tcgtttagat gacattcctc
     1861 ggtgagcatg gatttacttt tcaccatttt ttcgatgcca tgccggtgca ggatggaata
     1921 tacgatctca ttgtgctcgg tggcgtctga agcccagtgt accttaacgc cgtttgcgat
     1981 cgactcctgc tcaaatcggg ccaggtattg gctcagattc gaaagcacgt tgtcctttat
     2041 tcccgaagcc gtctcacgca acgcttccca gtcgggaacc ttcgaggcgg cgatgtccct
     2101 tttccggcgt acccaccaga gcgtctggtc gtgccagtcc acccgcggct cgtccttatt
     2161 gaatgccgcg gccagcgcgg catgatcttt atgctgtgtc attgctattc aggatttctg
     2221 cgatatgttt tacacggaca ttgctgttgg cgcggcggag gatgccctcg agatgcatca
     2281 gacagctcat atcggaggcc gtgatgaact ccgcgccatg tttgatatgg tcggcgcacc
     2341 ggtcctttcc catttttacc gagactgctt cttccgccac gcagaaagtt ccgccaaatc
     2401 cgcaacattc atcgacacga tcgagtccga tgagctcgag ccccctgacc atgctcagca
     2461 gccgctccag cttcgagaag ggcgccgcca tcagttcgct catctgtgca agatgcagac
     2521 cgcgttgccc atgacagctt tgatgaagac ccaccttatg gggaaatgtt gcttcgaggt
     2581 gttcgatctt caatatgtct gtgagaaatt cgacaagctc gaaaatcttt ttagggatcc
     2641 cggtatcacc atggaggtgt tccttgatat gcaatacgca gctgccggac ggggcgacga
     2701 tatagtcgaa gtcttcgaaa tttttgatga agagttcatt acagcctcct gtgaggtgtt
     2761 cgaatcccga attggccatg ggctgcccgc aacaggtctg atcgggaggg taaaccactt
     2821 cgcaacccag cttctccaat aattgcagcg tggcgacacc cacctgcgga tacaactgat
     2881 caatataaca gggtatgaat aatccaacac gcatcctatc ggtaatattt taattctata
     2941 atgtctgccg gcgggaaatg actgttccag tgttcatgga tcgccagggc cgcgcccata
     3001 gccgtcgcct gcgggatgga ggctgcgaag acctcggtat ccgggaaggc gtccgccagc
     3061 atatgcatat agacctggtt ccttccaaaa cctccgtcca cgaagatacg ccgtacgccg
     3121 gtatcatgga gcaccaattg cgtggactgc acctgttggg tcatgatgtc cgtcatcagc
     3181 aggtgatagg cttgctcgaa attgtcgtcg tccctccggc cgcgtcgaat ggcgtcaacg
     3241 caatccgcgt catacctgac cgtctccgca tacgccgggt ccttatgaaa acgagccgcc
     3301 agctccttca cccgctgctc atgctcataa ccggcaaaaa gacgcgaggc cttgacaggc
     3361 cgcccctgat agctcatata acaaagacaa tccttctgca gctcggccac cgtcagcggc
     3421 gagtcgttga aagggttcaa cgtgatacac catgtgccgg tggagataag cacgaacggc
     3481 tcgcggaagg tctggagata agggatcaac gcagccgagc tgtcatggag accgacaccc
     3541 gcgagcgata gtacgcccgt ggaaggcatt ataggagcca gcttatcgat gacaccctcg
     3601 cggtacaccc attcatgata atgctgttgc gcgaaattcc acaaattggt gtgacacccg
     3661 atgctggtga tgtcggaaca agccatccct gtgaccaacc agctaaggta ctgcggcaga
     3721 tgaagcgaga ccttgatcga atcgaaaaga tcgggccgct gcttttttat ccggtaaagc
     3781 tgcatccccg aattgagact gcccaggaca ggtgatgcag tcagcatcga aaaggtgacc
     3841 tcaccgccat aggtgtcata aaaagatttc tttagatctt caggatacgg tttcaaatag
     3901 ttgtataaag gcgccgccgg cgtcccgtcg aatccgatat gtacgagact ggcgccatag
     3961 gcggagaaat tcaccgcttt gatttggaga cccggcaatg cggctacctc cgccagcgaa
     4021 cgcttcaccc agtcggtgag cagcactaca tcttcacatg cgtcgccgtc ctcatcggtg
     4081 gtctgcggac atacctccga ccgctcccat acgatccggt attgttcgtc aaaaaggaag
     4141 agtttcttat ttgtttttcc tacgtcaaaa atggcaatga cgttctgcat aactataagc
     4201 cggtggctac tgtgccggcg cctctttctt tggtcaaatg ttcacgcact ttttcgatgc
     4261 gatagagtgc gagaggatcc aatgccgctc ccgcacggag cctcgcctcc gcgaccagcg
     4321 cgcgaacatc gctccgaaaa ctattctgaa ggacctcctg tgcagccaca gtatcattgg
     4381 cctgctgcgc ggcgagcagc cgcggccggt cgaccagcaa ggcctgcgcg taggtgatca
     4441 ggatcgcttc cacggactgc agcaggtctt ccaacggatc tttgacattg tgtgaagcgt
     4501 cgatcatcca gcccaggtcg cgtgcatgat ccataccacg cgcgtccatt ccttccacca
     4561 gctcgttaaa gatgaggaac agctgatagg gcttgatgct gcctaccgtc agatcgtcgt
     4621 caccgtattt ggaatcgttg aaatggaaac cgccgagctt tccttccatt aacaataaag
     4681 ccacgatctg ctcgatattg gcgttaggga ggtgatgacc gagatcgaca agcgtaaatg
     4741 ccttcggtcc cagtttggat gcgtatagca atgactggcc ccaatcgccc accgtcgtgg
     4801 aatagaaatt cggttcatag gccttgtatt cgacgaagac cttccaatcg tccggtaagg
     4861 cgtcgtatat ttcgcgcaaa ccttccaggg tgttttgaaa agcctggcgg aaattcaact
     4921 gacccggaaa acatgagccg tccgatagcc agacggtcag agacttcgag ccgagttcga
     4981 cgccatattt gatcacttct ttattgtgtt cgatggcctg acggcgtacc gcctttttaa
     5041 cgttttggag cgagccgaat ttgtaagtct gctcctgtcc gggctgatcc tggaaggtgt
     5101 tggagttcat cgcgtcaaag gtcagcccat gctgggcggc gagtgctttt atggatgcgg
     5161 ggttgcgggg gatatcccag ggaatatgca atgatatcgc gccgctgctg cggttgaggg
     5221 aatgcagcaa ccccacgtcc tcgatctttt cttcgagact ccgcggctcg ccgccgcccg
     5281 agaaccggcc aaaacgggtc ccacccgttc ccaatgccca actgggtatc gctacctgga
     5341 aatcgatgag cttttggatg atcgcttcgg catccttcaa cccgccggcc aggaattcga
     5401 attttcggcg atggtctttc tccgccgaat ggttgtgctc gtctatttta tatttttcta
     5461 tttgcatgtt tctctgtttt aggcattcgc ccccacctgt atccctatca ggtctaccag
     5521 ggggctaagg catataaaaa acttcattca gcggtcgggt cacaggggaa tgatccgggt
     5581 tagtctccat gatgtcctgc atatacgccc accagcgttg catcaccggc tgggcgggaa
     5641 gactgtccag ttttgccgcg tcgtcgatac acagcgctgc gaataaggtc agtgttgtct
     5701 catccaaaaa tat
//
LOCUS       DASYSV010000549         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_622589, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000549 DASYSV010000000
VERSION     DASYSV010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622589"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..941)
                     /locus_tag="VGQ51_17090"
     CDS             complement(132..941)
                     /locus_tag="VGQ51_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV9038356.1"
                     /translation="MSSLTITTIQTSLVWEDKDANLRLLEKKILSLPDPVELVILPEM
                     FSTGFSMKPEFFAETMDGPSVSWMKAIAARKRIILTGSLIMREGNDYFNRLLWMQPDG
                     VFGYYDKRHRFAYAGEDESYTAGRSRLITSVKGWRVLPLICYDLRFPVWSRQTPSDIQ
                     DGTGAAYEYDLLLYVANWPQRRAHAWKTLLPARAIENQCFVAGVNRIGEDGNSISHSG
                     DTQIIGPLGDVLYDAADRDEVFSLTLQKETLTGVRQHLPFWRDADHFTIES"
     gene            complement(960..1427)
                     /locus_tag="VGQ51_17095"
     CDS             complement(960..1427)
                     /locus_tag="VGQ51_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEV9038357.1"
                     /translation="MKRDASLSLEQALQKARHYCAYQERCHREVQEKLYSLGLRRTQV
                     EEALAVLIDENYLNEQRFAIQFAGGRFRMKHWGMVRIRYELKQKGISDHCIKKALETI
                     DEQAYERSLIRLAEQKWETLRDETDPLIRRRKLQAYLLQKGYESDRIAAVLPK"
     gene            1581..2696
                     /gene="dnaN"
                     /locus_tag="VGQ51_17100"
     CDS             1581..2696
                     /gene="dnaN"
                     /locus_tag="VGQ51_17100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV9038358.1"
                     /translation="MKFIVSSSALLKQLQQISGVINANTVLPILEDFLFEVEKNKLTV
                     VATDLETVMKVHLEVEAKDSGKVCIPARILLDSLKNIPDQPLTFNIDKHYAVELTSDN
                     GKYKVMGENPDNFPKEPASDETTSFTTTSSALVTAINKTLFAVSNDDLRPAMTGVYFE
                     MDKKGLQFVATDAHRLVRYRRKDVSCPKSDSLIVPKKPLNLLKSALPLNQDEITISYN
                     SNHLFVSHGTTQMSCRLIDARFPDYKVVIPADNPYKLTIGRNDFQSALRRVSIFSNKS
                     TNQVVLSIHGSELQLTAQDVDFSFEGNERMKCQYDGEDLNIAFNARFLIEMLASADSD
                     DIRMELSTPTKAGIIRPTEEDENEEVLMLVMPLMLNS"
     gene            2737..>3538
                     /locus_tag="VGQ51_17105"
     CDS             2737..>3538
                     /locus_tag="VGQ51_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEV9038359.1"
                     /translation="MHSIANYFAHIPSLQRSLILGGGLLLFWMLEGVVPLVRFRRHWG
                     RHTGLNIFFTLTTIIVNFAFASLIIRAADYCTEHHFGILYLVPLPFWLFTLLGLMLLD
                     LIGAWFIHWLHHKIKPLWQLHLIHHSDTWVDTTTANRHHPGESFFRAAFTLLAVGVAG
                     APAWLVLLYQSLSALFSQFNHANISLPKWLDIALGWVIVSPDMHKVHHHYKRPLTDSN
                     YGNIFSCWDRLFGTYNFVKDTAGLHYGIDTHPEAAEHNKLGPLLGMPFR"
BASE COUNT          809 a         1004 c          869 g          856 t
ORIGIN      
        1 atggccagcc ggtccacgat gtccacgatc gtaccttctt tgatcaaaac agctttaccc
       61 gtctcgatct gccgcccggt aaagatctcg cgggctacta atgcgatagc catagcaggt
      121 tctttaggtt tttaggattc tatcgtaaaa tgatcggcat cccgccaaaa cggtaagtgt
      181 tgccgaactc ctgtcagggt ctctttttgc agcgtgagac tgaatacctc gtctctgtcg
      241 gccgcatcat aaaggacatc tcccagaggt ccgataattt gtgtgtcgcc gctatgagag
      301 atgctgttac catcctcgcc gatccggttc actccggcta cgaaacactg attctcgatc
      361 gcgcgggcgg gcaaaagcgt cttccatgca tgagccctcc gctgtggcca gttggcgaca
      421 taaagcaaca ggtcgtattc gtaagcagct ccggttccgt cctggatgtc ggacggcgtc
      481 tgacggctcc atacggggaa ccgcagatcg tagcagatca acggcaacac cctccaaccc
      541 tttacggagg tgatcagtcg tgagcgaccc gcggtatagc tttcgtcctc gcccgcatag
      601 gcaaacctgt gccgcttgtc ataatatccg aagactccgt cgggctgcat ccagagcagc
      661 cggttgaaat aatcatttcc ctcccgcatg atgagactgc ctgtaaggat gatcctcttt
      721 cttgccgcga tcgccttcat ccaggagacg ctcggaccat ccatcgtctc ggcgaagaat
      781 tccggcttca tcgagaaacc cgtgctgaac atttcgggca ggatcaccaa ttctaccgga
      841 tcgggaagcg aaaggatctt tttttcaagc agccgcaaat tggcgtcctt atcttcccag
      901 accaggctgg tctgaatggt agtgatcgta agagaggaca tgtctgcaat gtaatatatt
      961 tatttgggaa ggacagcggc gatccggtcg gattcatagc ctttttggag gaggtatgcc
     1021 tgcaatttcc ttctccggat cagggggtcc gtttcatcgc ggagcgtctc ccatttttgc
     1081 tccgccagcc ggatcaatga ccgctcgtat gcctgttcat cgatggtctc cagcgccttt
     1141 ttgatacaat ggtcggatat ccccttttgt ttcagctcat accggatacg gaccattccc
     1201 cagtgcttca tccggaaacg tccgccggca aattgtatcg caaaccgctg ctcattcaga
     1261 taattttcgt cgatcagcac cgccagtgcc tcctccacct gggtccggcg caatcccaaa
     1321 gagtatagct tttcctgcac ctccctgtga caccgctcct ggtaggcgca gtaatgacgg
     1381 gccttttgca aggcctgttc taaagaaagt gaagcatctc ttttcataat tgattggtat
     1441 ccgcgaattt acgtccagaa gcgttcattg ccgccggtta gtcacaagaa attaacaatc
     1501 cgcctgccgc acgccggcgt gcgttctaat ctattggctg ttacgtaaaa aaacgtagtt
     1561 tcgccacaaa gacaaattcc atgaagttta tcgtttcctc ctcggcatta ttgaaacagc
     1621 tgcaacagat aagtggtgtg atcaacgcga ataccgtatt acccattttg gaagattttt
     1681 tgttcgaagt ggaaaagaat aagctgacgg tcgtcgctac cgatcttgaa acggtgatga
     1741 aggtacatct cgaggtcgaa gcaaaagact caggcaaggt ctgtatcccc gcccgcatcc
     1801 tcctcgactc gctgaagaat atcccggacc agccgttgac cttcaacatc gataaacact
     1861 atgccgtaga gctgacaagc gacaatggga aatacaaggt catgggtgaa aaccccgaca
     1921 acttccccaa ggagccagcc agtgacgaga ccacatcttt cacgactact tcttccgcgc
     1981 tggtcacggc catcaacaag acccttttcg ccgtgagcaa cgacgatctg cgtcccgcca
     2041 tgaccggtgt ctatttcgaa atggataaaa aaggtctgca attcgtggcg acggatgccc
     2101 accgtcttgt acgttacaga aggaaagatg tgtcctgtcc gaaaagcgat agcctgatcg
     2161 tgccgaaaaa acctctgaac ctgctaaagt cggcgttgcc gctgaaccag gacgagatca
     2221 ccatcagcta taacagcaat catcttttcg tctcacacgg aacgacacag atgagctgcc
     2281 gtctcatcga cgcccgtttc cccgattata aagtcgtgat ccccgccgac aatccctata
     2341 aactaacgat agggcgtaat gattttcaga gtgcgcttcg gcgggtcagc atcttcagta
     2401 ataagagcac taaccaggtg gtcctgagca ttcatggcag cgagctccag ctgacggcac
     2461 aggacgtgga cttctcattc gaagggaacg agcggatgaa atgccagtat gacggcgaag
     2521 atcttaacat cgcgttcaat gcccgcttcc tgatcgaaat gctggcctcc gccgacagtg
     2581 acgatatccg tatggagctg tccacgccta ccaaagccgg catcatcagg ccgacggaag
     2641 aagatgagaa cgaagaggta ctgatgctgg tgatgccatt gatgctgaat agctgatcac
     2701 acgttttttg cgtaaattac gtggaatcgc aagcccatgc attccatcgc caattacttc
     2761 gcgcatattc catccctgca acgctcgctt atcctcggtg gaggccttct gttattctgg
     2821 atgctcgaag gcgtcgttcc tttggtccgg tttcgccggc actgggggcg gcatacgggt
     2881 cttaatatct tctttacgct gacgaccatc atcgtcaact tcgcgttcgc ctcgctgatc
     2941 attcgcgcgg ccgactactg cacggaacac catttcggga tcctgtacct ggtgccgcta
     3001 cccttctggc tgttcacgtt gcttggcctg atgctcctcg atctcatcgg cgcctggttc
     3061 attcactggc tgcatcataa aataaaaccg ttgtggcagc tgcacctgat ccaccacagc
     3121 gatacgtggg tggacaccac gacggccaac cgtcatcatc ccggggaaag tttcttccgc
     3181 gccgcattca cgctgctcgc ggtaggtgtg gccggagcgc ctgcatggct ggtcctgctc
     3241 taccagtccc tgtccgccct gttctcccag ttcaatcatg ccaacatctc gcttccaaaa
     3301 tggctggaca ttgcgctggg ctgggtcatc gtctcgcccg atatgcacaa ggtgcatcac
     3361 cattacaaac ggccgctgac cgatagcaat tatggtaaca tcttctcctg ctgggatcgt
     3421 cttttcggga cctacaattt tgtgaaggat acggcgggcc tgcattacgg tatcgacacc
     3481 catccggagg cggccgaaca caacaaactg gggccgttgc ttggcatgcc gttccgac
//
LOCUS       DASYSV010000550         6761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2418749, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000550 DASYSV010000000
VERSION     DASYSV010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2418749"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /gene="mraW"
                     /locus_tag="VGQ51_17110"
     CDS             complement(<1..449)
                     /gene="mraW"
                     /locus_tag="VGQ51_17110"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEV9038360.1"
                     /translation="MTTPDQDKDQSAYHLPVLFHESLDALAIQPDGVYVDCTFGGGGH
                     SRGILGRLGPAGRLFAFDQDSDARRNLPEDNRISFLPHNFRHLTRMLRLHEAPPIDGI
                     LADLGVSSHQFDEAGRGFSFRFEGDLDMRMDRRQTLTAFDIVNGWSE"
     gene            complement(446..907)
                     /gene="mraZ"
                     /locus_tag="VGQ51_17115"
     CDS             complement(446..907)
                     /gene="mraZ"
                     /locus_tag="VGQ51_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEV9038361.1"
                     /translation="MVGFLGEYEATMDSKGRFLLPVGFKKQLPEDSVAQFVINRGFEK
                     CLSLYPMKSWEPIFARISQLNDFDPEVRQFRRYFLNGATIAELDSAGRLLIPPNLKEH
                     AGLEKDIVLAAAVDKIEIWDKSKYQQFFESFSPEAFSSLARQVMNPGGPAS"
     gene            1111..1302
                     /locus_tag="VGQ51_17120"
     CDS             1111..1302
                     /locus_tag="VGQ51_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038362.1"
                     /translation="MRKAIFNVNNLYYATACRRGKATDLTEDVVRNYALANGLVDIKV
                     CAVSEEFSGLKLVVRLKDR"
     gene            1305..2516
                     /locus_tag="VGQ51_17125"
     CDS             1305..2516
                     /locus_tag="VGQ51_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEV9038363.1"
                     /translation="MNVKDISIAGFSYELPAERIAYYPLAERDGSKLLIYSAGAINED
                     IYRNIAGYLPQGSLMVFNNTKVVEARILFQKPTGGQIEIFCLEPSSDAGGMAAALART
                     GTVQWKCLIGGASKWKRGVVLQKKVGDGVLEARYLEKSGDAFLIGFSWTPAESSFAEV
                     LHRMGNIPLPPYIHRQAEASDSQRYQTVYAQYDGSVAAPTAGLHFTETIFRSLDARGI
                     RRDFVTLHVGAGTFLPVKTATLGEHPMHVEFIEVSREFILRLRDTLGSGKPVIAVGTT
                     SARTLESLYWLGAGGDGLVVRQWDPYQPESRGIDAVEALSGLLLRMEEKGLSRLVTTT
                     QLLIMPGYRWRIVNGLVTNFHQPESTLLLLVASLIGDDWRRVYAYAMEHDFRFLSYGD
                     GCLLLPAGTQE"
     gene            complement(2467..3849)
                     /locus_tag="VGQ51_17130"
     CDS             complement(2467..3849)
                     /locus_tag="VGQ51_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV9038364.1"
                     /translation="MGLKKIFPIIFVLITLSLIGSIYIQINWILAMLQNKREDLYRKV
                     VLAIDNVTQDLVQQRSLSTGKTLRLKPSIPWRASDPFTMELMRVPLVAERYTADEIHD
                     RLRKAFDKMGLKNTRFEFSISSDLNTADLSSPGIFELQSRNFMKEIQDTTNKQFFFQP
                     IQAPENADGNSILPDETFVVVIPDFKNIVLGEMKAMIGGAIFFTLVIIAAFYVTVNAL
                     VRQKKLSEIKNDFINNMTHEFKTPLATISLAVDALRNEKVVHDREKSEYFTGIIKEEN
                     KRMNKQVETILQASLLDRQEQQLNRKRLHAHSIIQEAMENFQLQLEGKGGRSELQLNA
                     KCDWIEADEVHFMNIITNLIDNAVKYSKESPLIRCVTHSTAKNLVIRIEDNGIGMSKE
                     TMRRIFEKFYRAHTGNLHNVKGFGLGLSYVKTIVEAHGGKIKVESVVGKGTNFTLEFP
                     LATKDNHLHS"
     gene            4068..4634
                     /locus_tag="VGQ51_17135"
     CDS             4068..4634
                     /locus_tag="VGQ51_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEV9038365.1"
                     /translation="MIEDMESPAPGILLIADPFLKDPNFMRTVVFLCEHQDEGSFGFV
                     LNKNYEYTLDELVSGLDDLTKIPVYLGGPVQMDTIHFLHQYPDKISGGYEIIDGVFWG
                     GNFETAIDLIRQGEIDMSKIRFYIGYSGWGSGQLADELKEKSWLTVQASRRLVFHKKP
                     DEIWKDALRHLGGDYEMMVNFPTDPQLN"
     gene            complement(4646..4909)
                     /gene="atpC"
                     /locus_tag="VGQ51_17140"
     CDS             complement(4646..4909)
                     /gene="atpC"
                     /locus_tag="VGQ51_17140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEV9038366.1"
                     /translation="MNLEILTPEKKLFSDDVYGVQLPGVDGMFEVLDKHAPLVGALKE
                     GRVKVLRDKHNLAFFTIQSGFVEVIHNTVTVLVEGAEPVEGKA"
     gene            complement(4921..6420)
                     /gene="atpD"
                     /locus_tag="VGQ51_17145"
     CDS             complement(4921..6420)
                     /gene="atpD"
                     /locus_tag="VGQ51_17145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV9038367.1"
                     /translation="MASNGKIKQIIGAVIDVQFDGKLPEIYNALELKKTNGETLVLEV
                     QQHLGEDSVRCIAMDGTEGLVRGLPVNDTGNAIAMPIGEKIKGRLFNVTGDAIDGLPA
                     VDKTGGRPIHNKPPAFENLSTSTEILFTGIKVIDLIEPYAKGGKIGLFGGAGVGKTVL
                     IQELINNIAKAYSGLSVFAGVGERTREGNDLMREMIEAGIMKYGEDFKHSMEHGGWDL
                     GKVNLDELSESKATFVFGQMNEPPGARARVALSGLTIAEYFRDGDGKSQGRDILFFVD
                     NIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLMQERITSTKNGSITSVQAVYVPA
                     DDLTDPAPATTFAHLDATTVLSRKIADLGIYPAVDPLDSTSRILTPKIVGDAHYNCAN
                     RVKLILQRYKELQDIIAILGLDELSDDDKMTVSRARKVQRFLSQPFHVAEQFTGLKGV
                     LVPIDETIRGFNMIMDGEVDEYPEAAFNLVGNIDDAIEKGKKLLVQAQG"
     gene            6560..>6761
                     /locus_tag="VGQ51_17150"
     CDS             6560..>6761
                     /locus_tag="VGQ51_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038368.1"
                     /translation="MQLIEVNTPALAKEFILINVQLNRANPSYIRPIDKEISEVFDPA
                     YNKAFRNGEVTRWILRDEEGRPI"
BASE COUNT         1519 a         1652 c         1904 g         1686 t
ORIGIN      
        1 tgttcgctcc agccattgac gatgtcgaag gcggtgagcg tctggcgtct atccattcgc
       61 atatcgagat cgccttcaaa acggaaagag aaacctcttc cggcttcgtc gaactgatgg
      121 ctgctgactc ccagatccgc aaggatgccg tcgatgggcg gagcctcatg caggcgaagc
      181 atgcgggtca ggtggcggaa attgtgcggc aggaacgaga tgcggttgtc ctccggcaga
      241 ttgcggcgcg catcactgtc ctggtcgaat gcaaataatc tgccggcagg tccgagacgt
      301 ccgaggatgc cgcgggagtg accgccgccg ccaaaggtgc aatcgacata gacaccatcg
      361 ggttggatag ccagtgcatc gagcgactcg tgaaaaagga ccggcaggtg ataagccgac
      421 tgatccttgt cctggtcagg ggtcgtcatg aagcaggccc tcccgggttc attacctgcc
      481 tggcgaggct gctgaatgct tccggtgaaa aagattcaaa gaactgttgg tacttacttt
      541 tatcccagat ttcgatcttg tctacggcag ccgccaaaac aatgtccttt tcgagaccgg
      601 cgtgctcttt cagattgggt gggatcaaca accgcccggc agaatccaat tcggcgatcg
      661 tcgcgccgtt gaggaaatat cttctgaatt gacgaacttc ggggtcaaaa tcattcagtt
      721 ggctgatacg ggcaaaaatc ggttcccagc ttttcatcgg gtagagactc agacatttct
      781 caaaaccgcg gttgatgaca aattgggcga cactgtcttc cggaagctgc tttttgaagc
      841 caaccgggag caggaagcga cctttcgagt ccatagtagc ttcgtattcg cccagaaaac
      901 caaccattta tgaggaattg agtcaatttt tactaaaact aacacaaaat aacacttttt
      961 cccactttat taacggaaaa tgaaaaatta aatttttcca cagtagtcca ttggcgctgt
     1021 gtgtgttgtc cgggttgggt tggcgcgaac gcggaggctt ttttggtggt tttcgaccgt
     1081 gcagagtgtg tgaattgcag tggataagat gtgcgtaaag ccattttcaa tgtgaataac
     1141 ctttattatg ccacggcgtg ccgccggggc aaagccaccg atctgacaga ggacgtagta
     1201 aggaattatg cgttggccaa cgggctggtg gatatcaaag tgtgcgcggt cagcgaggag
     1261 ttcagcggcc tgaagctggt ggtgcgttta aaggacagat agatatgaat gtaaaagata
     1321 tttcgattgc cgggttcagc tatgagctgc cggccgaacg gatcgcgtac tacccgctgg
     1381 ctgaacgcga cggcagcaaa ttactgatct attccgcggg cgcgatcaac gaagatattt
     1441 accggaatat cgcgggatat ttaccgcagg gatcattgat ggtgttcaac aataccaaag
     1501 tggtcgaggc ccggatcctt tttcaaaaac ctacaggcgg gcagatcgag atcttttgcc
     1561 tggagccttc ttccgatgcc gggggaatgg ccgcggcgtt ggcacggacc gggaccgtgc
     1621 aatggaaatg tctcatcggc ggtgcgtcca aatggaagag gggagtcgtt ttacaaaaaa
     1681 aagtcggtga cggcgtactg gaagcacggt acctggaaaa atcgggggat gcgtttttga
     1741 tcggattttc atggacgccg gcggaatcgt cttttgccga agtgctccat cggatgggga
     1801 atatccctct cccaccctat atccatcgcc aggcagaggc ttcggacagt cagcggtatc
     1861 agaccgtcta tgcacaatat gacggatcgg tggcggcgcc gacggcagga ctgcatttta
     1921 cggagacgat ctttcgctcg ctggacgcac gcggtatccg tcgcgacttc gtcacgctgc
     1981 atgtcggggc cgggactttc ctgccggtga agacggccac attgggggag catcccatgc
     2041 atgtcgaatt catcgaggtt tcgcgcgaat tcattctccg tctacgggac accctgggca
     2101 gtggaaaacc cgttatcgca gtgggaacta cgtcggcgcg aacgctggaa agtctttatt
     2161 ggctgggagc cggtggagat gggctcgtcg ttcggcaatg ggatccctac cagccggaga
     2221 gccgcgggat cgacgcggtc gaggcgttat cggggttgct gctacgcatg gaggaaaaag
     2281 gattgagccg gctggtcacc acgacacaat tgctgatcat gcccggctat cgctggagga
     2341 tcgtgaatgg tcttgtcacc aatttccacc agcccgagtc gaccttgtta ctgctggtgg
     2401 cctcgctgat aggcgatgat tggcggcgcg tatatgctta tgcgatggag catgatttcc
     2461 ggttcctcag ctatggagat ggttgtcttt tgttgccagc gggaactcaa gagtaaaatt
     2521 ggttcccttg ccgacgacgc tttccacctt gatctttccg ccatgggcct cgacgatggt
     2581 cttcacgtag ctgagcccga gaccgaagcc ttttacattg tgcaggttgc ccgtatgtgc
     2641 gcggtaaaac ttctcgaaga tgcgacgcat cgtttctttg ctcatgccga taccattgtc
     2701 ctcgatacgg atgacgagat ttttggccgt actatgtgtg acgcaacgga tcagcgggct
     2761 ttctttcgaa tatttcaccg cattatcgat gaggttggtg atgatgttca tgaaatggac
     2821 ctcgtcggcc tctatccaat cgcattttgc attgagctgc agttccgatc ggccaccctt
     2881 gccttccagc tgcagctgaa aattctccat agcctcctgg ataatgctat gcgcgtgcag
     2941 ccgcttgcgg ttcagctgtt gctcctgacg atcgagcagg gaggcctgca ggatggtctc
     3001 cacctgtttg ttcatcctct tattctcctc tttgatgatc ccggtgaaat attccgactt
     3061 ttcacggtcg tgtaccactt tctcgttacg aagcgcgtct acggcaagcg agatcgtggc
     3121 cagcggcgtt ttgaactcgt gggtcatgtt gttgatgaaa tcgttcttta tctcgctgag
     3181 ctttttctgg cggacgaggg cgttgacggt aacatagaag gcggcgatga tgaccagggt
     3241 gaaaaagatg gcgccgccga tcatggcctt catttcgccg aggacgatgt tcttgaaatc
     3301 gggaatgacg acgacgaagg tctcatcggg caggatagaa ttgccgtccg cattttccgg
     3361 ggcctggatc ggttggaaga agaattgttt gttcgtcgtg tcctggatct ctttcatgaa
     3421 attcctggac tgcaactcga agatcccggg actgctcaga tcggctgtat tcagatcgga
     3481 ggagatcgag aattcgaaac gggtattttt caagcccatc ttatcgaagg ctttgcggag
     3541 acggtcgtgg atctcgtcgg cggtgtatcg ttccgctacc agcggcacgc gcatcagttc
     3601 catggtaaaa ggatccgagg ctctccaggg aatgctcggt ttcaaccgaa gggtcttgcc
     3661 ggtggacagg ctcctttgct ggaccaggtc ctgggtcacg ttatcgatgg ccaataccac
     3721 cttgcggtac aggtcttccc gtttattctg cagcatggcc aatatccagt tgatctggat
     3781 atagatgctt ccgatcaggg acagagtgat caatacaaag atgataggga aaattttttt
     3841 caaacccata tattacttta ctacacgctg tgttttggtc caactgcaaa tttattgaaa
     3901 ggatgaaggg ttcccttttg agcccttccg ggaaaacgct atttaacgaa ggtttcacaa
     3961 agaaaggggt tttcgccctt aaaatgcact atatttagta tcgaggcccg ccggccaccg
     4021 aaaaagcacg ctttttgtta tccgcaggat tacttaattt agatggaatg atagaagata
     4081 tggagagccc ggcacctggc atattattga tcgctgatcc attcctcaaa gatcccaact
     4141 tcatgcgtac ggtagtgttc ctgtgcgaac accaggatga aggcagtttt ggtttcgtac
     4201 tgaataagaa ttacgaatat acattggacg agctggtcag cggtttggac gacctgacaa
     4261 aaataccggt gtacctcggc ggtccggtcc agatggatac cattcatttc ctgcatcaat
     4321 atcccgacaa gatcagcggc ggatacgaga tcatcgacgg tgttttctgg ggaggcaatt
     4381 tcgagacggc gatcgatctg atccggcagg gggagatcga tatgtccaag atccgttttt
     4441 acatcggcta ttcgggatgg ggaagcggtc agctggcgga cgagctgaag gagaagtcct
     4501 ggctaacggt acaggccagc cggcggctgg tattccataa gaagcccgac gaaatatgga
     4561 aggatgcgtt acgccatctc ggcggggatt atgagatgat ggtgaatttc ccgaccgatc
     4621 ctcaattgaa ttagtaaccc cgggcttacg ctttgccttc gacaggctcc gcgccctcga
     4681 ccaacacggt aacggtgttg tgaatgactt cgacgaaacc gctttggatc gtaaagaagg
     4741 caagattgtg tttgtccctg aggaccttga cccttccttc tttgagtgcg cccaccagcg
     4801 gagcatgttt gtccaatacc tcgaacattc catctactcc gggtagttgt acgccatata
     4861 cgtcgtcact gaacagcttc ttttcgggtg ttaatatttc taggttcatt gtttcttggt
     4921 ttaaccctgt gcctgcacca ggagcttctt acctttttcg atggcatcgt cgatgttgcc
     4981 gacgaggttg aatgcggctt cggggtattc gtccacctca ccgtccatga tcatgttgaa
     5041 tccgcggatc gtctcatcga tgggaacgag cacgcctttc agaccggtga actgttcggc
     5101 cacgtggaag ggctgggaaa ggaaacgctg taccttacgg gcgcgggata ccgtcatttt
     5161 atcatcgtcg ctcaattcat cgagaccgag gatcgcgatg atatcctgca gttccttata
     5221 gcgctggagg atgagcttga cgcggttggc gcaattgtaa tgagcgtcac cgacgatctt
     5281 cggggtgagg atccgcgagg tagaatccag gggatcgacc gcaggataga taccgagatc
     5341 ggcgatctta cggctcaata cggtggtggc atcgaggtga gcgaatgtcg tcgccggtgc
     5401 gggatcggtg aggtcatccg cgggaacgta tacggcctga acagaagtga tcgacccatt
     5461 tttagtggag gtgatccttt cctgcatgag acccatttcg gtcgcgagcg tcggctggta
     5521 tccgacggca gagggcatac ggccgagcaa tgcggaaact tccgatcctg cctgcgtgaa
     5581 acggaagata ttgtcgacga agaacaggat atcacgaccc tggcttttcc cgtcgccgtc
     5641 acggaaatat tccgcgatgg tcagaccgct gagtgcgaca cgggcgcggg ctccgggggg
     5701 ttcgttcatc tggccgaata cgaaggtggc ctttgattcc gacagttcat ccagattgac
     5761 cttacccaga tcccatccgc catgttccat gctatgtttg aagtcttcgc cgtatttcat
     5821 gatgccggcc tcgatcattt cgcgcataag gtcgttgcct tcgcgtgtac gctcgcccac
     5881 gcctgcaaaa acggataaac cggaataagc tttcgcgata ttgttgatca attcctggat
     5941 aagtacggtc ttgcctacgc cggcaccacc gaacagaccg atcttaccgc cttttgcata
     6001 gggctcgatg aggtcgatga ccttgatacc ggtgaaaagg atttcggtac tggtgctcag
     6061 gttttcgaag gccgggggtt tattgtgtat gggtcggccg ccggtcttat ccacagcagg
     6121 aagaccatcg atggcgtcgc cggtgacatt gaaaagcctg cctttgatct tttcgccgat
     6181 gggcatggcg atggcgttgc cggtatcgtt gacgggaaga ccgcggacca gaccttccgt
     6241 accatccatg gcgatgcaac ggacgctatc ctcgccgagg tgctgctgaa cttccaaaac
     6301 gagggtttcg ccattggtct ttttcagttc gagcgcgtta tagatctcag ggagcttgcc
     6361 atcaaactgc acatcgatca ccgctccgat gatttgcttg atcttaccgt tacttgccat
     6421 ataagttttg tctgttttta ggtgttgccg gccgccattg aaatccggcg ggcaggcagg
     6481 ttttaaattc ggctgcaaag gtaaggggtg acagggaaag ttaaaaatgg ttatttttgt
     6541 cgtttaccaa catcggctta tgcaattgat agaagtcaac accccggcgc tggcgaagga
     6601 attcatcctt ataaatgttc aactcaaccg cgccaatcca tcctatatac ggccaatcga
     6661 taaggagatc agcgaggtgt tcgatccggc atacaacaag gcgttccgca atggagaagt
     6721 aacccgttgg atactaaggg atgaggaagg cagaccgatc g
//
LOCUS       DASYSV010000551         9759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1395482, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000551 DASYSV010000000
VERSION     DASYSV010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9759
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395482"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGQ51_17155"
     CDS             complement(<1..303)
                     /locus_tag="VGQ51_17155"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV9038369.1"
                     /translation="MRMVFIGLFLLSVAVARAQSQPISSAAIDSLAERTLRAFEVPGV
                     AVCVIKDGKVVHSKGYGVRSLATRQPVDENTLFGIASNSKAFTTAALGMLVDEGKLN"
     gene            complement(594..1238)
                     /locus_tag="VGQ51_17160"
     CDS             complement(594..1238)
                     /locus_tag="VGQ51_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038370.1"
                     /translation="MEGGKSFYSLDYIIELGEKRMEQYSTAYQLTLGRTTNIIVVYSA
                     VGIYLIPIVQDFGQKPSVVFEIETIVLVMILSCSLFYAIRLLIPANLAYLKVSSEYYQ
                     DMRLQYEQKMLHQEMSDSDRLKSEQKIDDFIKTAYIDEPAWGQQNNWTILTRKSLYYQ
                     KALVCGLLAILPYIVCVSYHISAQKAKTEKQGKTSQKIIKFVDTIAKNEGFKTR"
     gene            complement(1408..1950)
                     /locus_tag="VGQ51_17165"
     CDS             complement(1408..1950)
                     /locus_tag="VGQ51_17165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV9038371.1"
                     /translation="MTGKKNVFAIVGSASRHSSNERLVMKVAEMALDDFRITVFGDLK
                     TLPHFDPELSVGETPKVVRKFRADIEAADAVIICTPEYVFSIPSGLKNAIEWCVSTTV
                     FTDKSLGIITASADGQKGHEELQLIMRTLMASFTEETTLLIRGIKSRIDAEGAITDKQ
                     TEDSLRNFVTGLRRLADSGD"
     gene            2291..3676
                     /locus_tag="VGQ51_17170"
     CDS             2291..3676
                     /locus_tag="VGQ51_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038372.1"
                     /translation="MKRTWILRLCLTGNALFIIVLFFACKKQHETVPANPLASQIQSA
                     RDFFESTIHSAAAPPNAANYRAKLQKSIRWDLAQTTTLSGKPAIVAPVQFARRVYVSS
                     DLEPGTAFELSSLSRLVISLDSENVFHYGLLTYIPDSTAIQTQTLNTGILLYEDWQGN
                     SLSAPQRFARRHMAITNSDNKQIDIVQTIQVCNEIDGYNYAPDDPEGGLNTWSETSCN
                     TYTLPAMTTGPRIGPGSLPGVLGPRPIPSLTVVIAPPTSPITDITAYLKCFTNSTAID
                     HTYSVTVCASQPQAGTRDPWGFTQGGPAAMAAASNPINVGHTFLIFSENSAGTVITRN
                     VGFYPQSGVNPMSPSSQGQLDDNEATNYDISLTFTVSNTQFFSMLNYIAIGNNSGYLY
                     NLNSNNCTTFALHALSAGGESLPNTQGSWPGGSGNDPGDLGEDIRSMTLPPNATRNTV
                     QNAHPNQGSCN"
     gene            3698..4105
                     /locus_tag="VGQ51_17175"
     CDS             3698..4105
                     /locus_tag="VGQ51_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038373.1"
                     /translation="MKLSSILLFGFLAFNFCEGQSVQWSTTKNWKIYKGEGFQIFSYR
                     VDTLRQIRSQSLEKDSVLYYLQKAQPIPTEKTPVWMGEYLATYESPEGKTYKVEVSIY
                     GGFFCDQNSGKYYEVPFELRRQWLNYVTKNVPN"
     gene            complement(4254..4835)
                     /gene="nudK"
                     /locus_tag="VGQ51_17180"
     CDS             complement(4254..4835)
                     /gene="nudK"
                     /locus_tag="VGQ51_17180"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HEV9038374.1"
                     /translation="MSKTLVEVVGSEVLSDNWYVLRKISYRYRDPGGNWVTRVREVYD
                     RGNGAVILLYNKVMGTVILTRQFRLPTFVNGNADGMLIEACAGLLDKDNAEDAIRRET
                     EEETGYKVSALQKVYEAYMSPGSVTEILYFFIAEYTPSMKVHEGGGLEHEQEEIEVLE
                     IGFEEALRMVERGEIRDGKTIMLLQAVRLRGVM"
     gene            complement(4840..5334)
                     /locus_tag="VGQ51_17185"
     CDS             complement(4840..5334)
                     /locus_tag="VGQ51_17185"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEV9038375.1"
                     /translation="MRHATLSLTVLLWAVIATAQPVPGGPFLPTSFMPKMQVTTGIYP
                     HSVVAADLNGDGKPDLFVARGSSSSNCILPNTSSSGILSFGTPVVLANDGIDEEGAAA
                     GDPNGDGRIDIVVANGIGGPSVSIYENTGTSGSFAFTKIDLPVVVGLDGVNIGKTVKI
                     LKIE"
     gene            5510..6541
                     /locus_tag="VGQ51_17190"
     CDS             5510..6541
                     /locus_tag="VGQ51_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV9038376.1"
                     /translation="MKEGKLPTIKEIAKQLNVSVSTVSRALHDHPGIGLRTKMRVQQL
                     ARELDYEPNQTAIFLRQRKTFTIGVVLPNLKEEFFSEAINGIEDIATQHKYSVLISQS
                     KDDIERERRILATMKNNRVDGVIASLGAHTTSYDHFTALKKFGIPLVFFDRVPATPGF
                     NKVYSNMNSGTHEAIEFLIKKGHRRIAVLNGPEKMQSSLERTETYKQVLIKKRLKIDL
                     GLVAYTDLTRDSTHQAMQVLLRQRPRPTAILVINDYIALDAMQYARQQKLKINKDICF
                     VSYANLPITNYLENPPMASVEQYPYQQGARAAEILIQLLADNENSDPPPESFQSVVIN
                     GQLVIHSGR"
     gene            6615..7691
                     /locus_tag="VGQ51_17195"
     CDS             6615..7691
                     /locus_tag="VGQ51_17195"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV9038377.1"
                     /translation="MYDQILTAYGFPANTSITPHGSGLINHTWHVRSDKGDFILQRLN
                     NSVFSRPDFIAENIERVGQYLANHYPHRIFPRPIMSADGLTMIDAGPNGFYRVFPFIS
                     DSVTVDVVQTPDEAFEAARQFGGFTRMLAGFPAQSLHDTLPGFHDLTRRYRDFLDALK
                     NGLPGRIHSTEQLIEFLKAQQHLVTEYEHICSNPGFKKRVTHHDTKISNVLFDRYGKG
                     ICVIDLDTVMPGYFISDVGDMFRTYLPPVSEEEADLSLIQVRQEYFEAIVKGYLKEMS
                     DVLTDVEKQAFVYAGEFMLYMQALRFLTDYLCGDKYYATRYEGHNYIRALNQTTLLQR
                     FQEMAPTLKAIAGQLTGNPRPPAA"
     gene            7797..8309
                     /locus_tag="VGQ51_17200"
     CDS             7797..8309
                     /locus_tag="VGQ51_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEV9038378.1"
                     /translation="MAELATSVARHKKGRGVKKSKKLSTRVDLTPMVDLGFLLITFFI
                     FTTTMSLPKAMNLHLPMDSKDSMLTKRSAVITIIPGKDDRIYYYEGDDMTNLQEADFK
                     SIRGILLDKKRRTDPTWFQVIIKPTRDATYKNTVDVLDEMLIDEISHYSLVDVSPAEY
                     QVIDTLRQPH"
     gene            8363..9364
                     /locus_tag="VGQ51_17205"
     CDS             8363..9364
                     /locus_tag="VGQ51_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEV9038379.1"
                     /translation="MKKIIYIFLAAATIHTASAQSDPNHQNLALPARSGNPIFPGWYA
                     DPEAAAFDTAYWVFPTYSDKYSKQVFFDAFSSPDLVQWSKHPHILDTSVIHWAKRAMW
                     APAIALKGGKYYFFFAANDIQNDSSLGGIGVAVARKPQGPYKDYLGKPLIGKFHNGAQ
                     PIDQAVFKDDDGQYYMIYGGWKHCNIAKLRPDFKGFIPFPDGATFKEITPEDYVEGPY
                     MMKRGGKYYFMWSEGGWTGPDYSVAYAIGDSPTGPFRRIGKILQQDTTVATGAGHHSV
                     IHIPGTDDYYIVYHRRPLGETDANHRVVCIDRLEFTADGHIRPVRITKDGVAARPLQ"
     gene            complement(9425..>9759)
                     /locus_tag="VGQ51_17210"
     CDS             complement(9425..>9759)
                     /locus_tag="VGQ51_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038380.1"
                     /translation="LLPALGYDQRQRTGRGYTFGRFRGEDMLYGESEYRFPISAHTGI
                     LGGDIFLNCTSTSNKEANVHVLDYLRLGYGGGLRIMMDKKSRTRLVVDAGIADRSVGF
                     YLAAQESF"
BASE COUNT         2502 a         2960 c         1953 g         2344 t
ORIGIN      
        1 attgagtttc ccctcgtcga ccagcatccc cagcgcagcg gtcgtgaacg ctttgctgtt
       61 cgatgcgatg ccgaaaagcg tattctcatc caccggctgc cgcgtcgcca gcgatcgcac
      121 cccgtatccc ttcgaatgca caaccttccc gtctttgatg acgcagaccg cgacccccgg
      181 cacctcgaat gcccgcaacg ttcgttccgc cagactatcg attgccgcag agctgatcgg
      241 ttggctctgc gcgcgtgcaa cggcaaccga cagtaaaaat aatccaataa aaaccattct
      301 catagcaata gctttggcta aatatagcca taatcctcct acaaggcaaa aacaaagggc
      361 cgtgcggttg tcccgcgcgg cccccaaaat aaattgttat acttcacgcc ctaccacttc
      421 tcattttctc ataatcgcta tatggacgct ttttacccgg cttgaaagtc caaccattga
      481 accacatagg ctgagaagga attacctgcg aactatcgat gcccggcacc tgcgttacga
      541 ctctatcctc tccgtccgga ggttgccctt cgctctcctt cacatccaca tttctatctt
      601 gttttgaagc cttcattttt agctattgta tcaacaaatt taataatttt ttgtgaggtc
      661 tttccctgtt tttctgtctt tgctttctgc gccgatatgt gataagatac gcagactata
      721 tatggcaaaa tggctaagag tccacagacc agtgcttttt ggtaatacaa actctttctc
      781 gttaggatgg tccagttatt ttgctgaccc catgccggct cgtcaatata ggccgtcttt
      841 ataaaatcgt cgatcttctg ctctgatttt aaacgatccg agtcagacat ttcttgatgt
      901 agcattttct gttcgtattg caatcgcatg tcctggtaat attcactaga caccttcaga
      961 taggcaagat ttgccggaat cagtagccga atggcgtaaa ataaagagca acttagtatc
     1021 atcacaagta caatcgtttc tatttcaaat acaaccgatg gtttctgtcc aaaatcttga
     1081 acgattggga tcaaatagat cccaaccgca gaataaacaa cgatgatatt tgtcgttcga
     1141 ccgagagtaa gctgatatgc agtcgaatac tgttccatcc tcttttcacc aagctcgatg
     1201 atatagtcca gactatagaa tgatttgccc ccttccatag caacgggaat ttaggattta
     1261 actcttaatc acgcccacac attaggcgaa tccggaaaga ttaattagtc catggggagt
     1321 accagttaaa agagtaagaa actactcaaa gttacaatat gaataaaatc acaaagaaat
     1381 attttgattc ccgatttcct gccgttatta atcgccacta tcggccaatc gtctcaaccc
     1441 agtcacaaag ttccgcaagc tatcctccgt ttgtttatcc gttatcgccc cctcagcatc
     1501 gatcctgctc tttattcccc taatgagcag cgtggtctcc tccgtaaatg acgccatcaa
     1561 ggttctcatg atcaactgca attcttcatg gcccttttgt ccatccgccg acgcagtaat
     1621 aatccccaaa gacttatctg taaagacggt cgtggaaacg caccactcga tcgcattctt
     1681 aagcccgctc ggtatgctaa aaacatattc cggcgtacaa atgataaccg catccgcagc
     1741 ctcaatgtct gcccgaaatt tcctcactac ctttggtgtt tctcccaccg aaagctccgg
     1801 atcaaaatga ggcagcgtct tcaaatctcc gaatacagtg atcctaaaat cgtccaacgc
     1861 catctccgcc actttcatca ccagccgctc attcgaggaa tgtctgctcg cgctacctac
     1921 aatcgcaaaa acgttcttct ttcctgtcat attacccttt tatattttac cgcaatttaa
     1981 atgcaggcgg cgtaaaagat tatacaaaat tttccgcaat cgcaccccaa attttttccc
     2041 tacagatcat taatcgtaaa caattaggtg cctccccaaa ctcactgcgc atctacgttt
     2101 tacactccat tcgccctcac gcatcccgcg ccatttctct ttgttaatgt gatctatatc
     2161 actttcgata ctgtctgcct catttttctt tcattcaatt ccctttagcg ctacctttat
     2221 ctcagccgtc gacctgtgcc tcactcagcc tcacctaacc gttcccataa ccattaaacc
     2281 tattccattt atgaaacgca cctggattct cagattatgc ctaactggca atgcactatt
     2341 cattatcgtt ctattctttg cctgtaaaaa acagcatgaa acggtaccag caaacccact
     2401 cgcctcccaa atccaatccg cccgtgactt tttcgagagt accatccatt ctgctgccgc
     2461 gccgccaaac gcggccaact accgggctaa gctacagaaa tctatccgct gggatctcgc
     2521 tcagacgaca actctttccg gtaaacccgc cattgtcgcc cccgtgcaat ttgcccgtcg
     2581 cgtatacgtc tcatccgatc tggaaccggg aaccgccttc gaactctcca gcctgagccg
     2641 cctggtcatt tccctcgact ccgaaaatgt cttccactat ggtctgctca cgtatatccc
     2701 cgacagcacc gccatccaaa ctcaaaccct caataccgga atcctgctgt acgaagattg
     2761 gcaaggcaac tccctctccg ccccgcaacg ctttgcccgg agacacatgg ccattactaa
     2821 ctccgacaac aaacaaatcg acattgtcca gaccattcaa gtctgtaacg aaatcgacgg
     2881 ctataactat gcacccgatg accctgaagg agggctcaac acctggtccg agaccagctg
     2941 taatacttat acgcttcctg ccatgaccac cggtcccaga atcggccctg gcagtctgcc
     3001 cggcgtccta ggtccgaggc caattcccag tctcacagta gtaattgcac cgcccactag
     3061 ccccattacc gacattacag cgtatttaaa atgctttacc aattccaccg ctattgatca
     3121 cacttatagc gtaactgttt gcgcttccca gccgcaagcc ggcacacgag atccttgggg
     3181 cttcacgcaa ggggggccgg ccgccatggc tgctgccagt aatcctatca atgtcggtca
     3241 tacctttctg atcttttcag aaaattcagc cggcactgtc attacccgaa atgtcggttt
     3301 ttatccgcaa tcaggcgtga atccaatgtc cccctccagt caagggcaac tcgacgataa
     3361 tgaagcaaca aactatgata tctctctcac ctttacggtc tctaacaccc aattcttctc
     3421 catgctgaac tatattgcca ttgggaacaa ttccgggtac ctctataatc tcaattccaa
     3481 caattgcacg acatttgcac tacacgcgtt gtcagcagga ggcgaatcgt taccaaatac
     3541 acaaggttca tggcccggcg gttccggaaa cgatccaggc gacttaggcg aggatatccg
     3601 ttcgatgaca ttgcctccta atgcaaccag aaacacggtt cagaatgcgc atccaaatca
     3661 aggcagttgc aattaatcaa tctacatttc aactcgtatg aaactttcat ctatcctatt
     3721 attcggtttc ctggcattca acttctgtga agggcaaagt gtccaatggt cgaccaccaa
     3781 gaactggaaa atttataaag gagaaggttt tcagatcttc tcataccgag tcgatactct
     3841 taggcaaatc agaagccaat cgttggaaaa ggattctgtc ctctattatt tacagaaggc
     3901 tcagcccatc ccaacagaga aaactcctgt ttggatggga gagtatttgg caacctatga
     3961 gtcgcctgaa ggcaaaacgt acaaagtgga agtatcaatc tacggcggct tcttttgcga
     4021 ccagaattct ggaaaatatt atgaagttcc ttttgagctc agacgtcaat ggctcaacta
     4081 cgtaaccaag aatgtaccaa attaaggagc ctctattcaa tatgtaacct ctatttgctt
     4141 ttaaacggtt ggctcatgca agaattgtca aaatatgtaa ccttccatag gagctatccc
     4201 ctgcattagt ttgcaacttc tcctttaccc cgccctcaac accgcacacc catctacatc
     4261 acccccctca acctcaccgc ctgcaacagc ataatcgtct tcccatccct aatctccccc
     4321 ctctccacca tcctcaacgc ctcctcaaac ccgatctcca acacctcaat ctcctcctgc
     4381 tcatgctcca acccgccgcc ctcatgcacc ttcatcgacg gcgtatactc cgcaataaaa
     4441 aagtaaagta tctccgtgac cgaacccggc gacatatacg cctcatacac cttctgcaaa
     4501 gcagatacct tatatcccgt ctcctcctcc gtctcccgcc ggatcgcatc ctccgcattg
     4561 tccttatcca ataatcccgc acaagcctcg atcaacatcc catccgcatt cccgttcaca
     4621 aacgtcggaa gacgaaactg tctcgtcaat atcaccgtcc ccatcacctt attataaagc
     4681 aatatgacgg ccccattgcc gcgatcatac acctcgcgaa cacgagtcac ccaattcccg
     4741 cccggatccc gatatcgata agagatcttt cgcaacacat accagttatc ggacaacacc
     4801 tccgatccta cgacttcaac caaagtctta ctcataagtt cattcaattt tcaagatctt
     4861 taccgtcttc ccgatattca ccccatcgag accaacgact acgggaaggt cgatcttcgt
     4921 aaaggcgaac gaaccgctcg tgcccgtatt ctcatagatg gacaccgaag gcccccctat
     4981 accgttggcc accacgatat ctatcctgcc atccccgttc ggatcgccgg cagcagcccc
     5041 ttcttcgtcg atgccgtcat tcgccagcac caccggagtc ccgaatgaca atataccgga
     5101 actactggta ttcggtaaga tacaattgct gctggaactg cctcttgcca cgaacagatc
     5161 gggcttgccg tcgccattga gatcggccgc cactacacta tgtggataga tccccgtggt
     5221 cacctgcatt ttcggcataa agctggtagg cagaaaagga cccccgggca cgggctgggc
     5281 agtggcaatt accgcccaca agagtacagt cagggagagg gttgcgtgtc tcatatgagt
     5341 caaatttgga ggtagggcgt tcgaatgcaa gataatataa aaaatccgcc atctcaaccc
     5401 ggtcccgcac tgcccgccat agttcattct ttatgcgcga tcttccagtc ctactgaaaa
     5461 cggcttgtta tttaatattt ttttaatgcc cccttcgtat ttttgaaata tgaaagaagg
     5521 gaagctcccg actatcaagg agatcgccaa acagctcaac gtctccgtct ccaccgtcag
     5581 ccgggcgctg cacgatcacc ccggcatcgg cctacgaaca aagatgcgcg ttcaacagct
     5641 ggcccgcgaa ctggactacg aacccaacca aaccgccatc ttcctgcgac agcggaagac
     5701 ctttaccatc ggtgtagtcc tccccaatct caaggaagaa ttcttttccg aagccatcaa
     5761 cggcatcgag gacatcgcca cccagcacaa atacagtgtc ctcatcagcc agtccaaaga
     5821 cgatatcgaa cgcgaaaggc gcatccttgc tacgatgaag aacaaccgcg tcgatggcgt
     5881 tatcgcgtcg cttggcgccc acacgacgag ctatgatcat tttaccgcgc tgaaaaaatt
     5941 cgggattccc ctcgtgttct tcgatcgggt gcctgccacc cccgggttca ataaggtcta
     6001 ctccaatatg aacagcggga cccacgaagc gatcgaattc ctcataaaaa aaggtcaccg
     6061 ccgtatcgcc gtcctcaacg ggcccgaaaa aatgcagtct tccctcgaac gtaccgagac
     6121 ctacaaacag gtcctgatca aaaagaggtt gaagatcgat ctcggcctgg tcgcctatac
     6181 cgaccttacc cgcgatagca cccaccaggc catgcaggtc ctcctacgcc agcggccccg
     6241 cccgactgct atcctcgtta tcaacgatta tatcgcgctc gacgcaatgc aatatgcgcg
     6301 gcaacaaaaa ctgaagatca ataaagacat ctgtttcgtc agctacgcca atcttcctat
     6361 taccaattat ctcgaaaacc cgcccatggc ctcggtcgaa caatatccct accagcaggg
     6421 cgccagggcc gcggagatcc ttatccagct gctcgctgac aacgaaaaca gcgatccgcc
     6481 gcccgaatcc ttccaaagcg tcgtcatcaa cgggcaactg gtcatacatt cggggcgctg
     6541 atcgagaaca accgtttgcg tatggtcaac ccgtcgatct tacttaattt cggccattca
     6601 ttcaacaccc ttccatgtac gatcagatcc tcactgctta cggattccct gcgaacactt
     6661 ccattacccc gcatggcagt ggtcttatca atcatacatg gcacgttcgc agcgataagg
     6721 gcgatttcat cctccagcgt ctgaataaca gcgtcttttc ccgtcccgat tttattgccg
     6781 aaaacattga aagggtgggt caataccttg cgaaccacta cccgcacagg atattcccgc
     6841 ggccgatcat gtccgccgac ggtctgacga tgatagatgc gggacccaat ggcttctatc
     6901 gcgttttccc ctttatcagc gattcggtga ccgtcgacgt cgttcaaaca cccgacgagg
     6961 ctttcgaagc agcccggcaa ttcggcggat ttacgcgtat gctcgccggc ttccccgccc
     7021 aaagcctgca cgataccctg cccggtttcc atgaccttac ccgccgctac cgcgacttcc
     7081 tcgatgcgct gaagaacgga cttcccggtc ggatccacag cacagaacag ctgatagaat
     7141 ttctcaaagc ccaacaacac ctggtcaccg aatatgagca catctgctcc aacccaggtt
     7201 tcaaaaaaag ggtcacccat cacgatacca agatcagtaa cgtgctcttc gaccgatatg
     7261 gcaaaggcat ctgcgtcatc gacctcgata ccgtaatgcc gggctatttc atcagcgatg
     7321 tcggggatat gttcaggacc tacctcccgc cggtcagcga agaagaagcc gatctgtcct
     7381 taatacaggt aagacaggaa tatttcgagg ctatcgttaa ggggtacctg aaagagatgt
     7441 cggatgtcct taccgacgtg gagaaacagg ctttcgtcta tgcgggggaa ttcatgcttt
     7501 atatgcaggc gctccggttc ctgaccgatt atctgtgcgg ggataagtac tacgccacgc
     7561 gctatgaagg acacaactat attcgggctc ttaaccagac gaccctgttg caacgtttcc
     7621 aggaaatggc tccgacgcta aaagctatag ccgggcaact caccggcaat cctcgtccac
     7681 cagccgcatg atctcctcat aacgttgcaa atcgtccctc gtccccggcc agtgccggac
     7741 aaagtttaac tatttatgga atgatttcga ccccgcatct aatataaaaa gctcctatgg
     7801 ccgaactcgc cacctccgtc gcacggcata aaaaaggccg gggcgttaaa aaaagtaaga
     7861 aactctctac ccgcgtcgac ctaacgccca tggtcgatct gggctttctc ctgatcacgt
     7921 tcttcatctt caccaccacg atgagcctgc cgaaggccat gaatctccac cttccaatgg
     7981 attccaaaga ctcgatgctg acaaaaagat ccgctgttat caccatcata cccggcaaag
     8041 acgatcggat ctattattat gaaggagatg atatgaccaa ccttcaggag gccgatttca
     8101 aatctatccg cggcatcctt ctagacaaaa agcgaaggac agaccccacc tggttccagg
     8161 tcatcatcaa acccacgcgc gatgccacct ataagaatac ggtcgatgta ttggatgaga
     8221 tgctgatcga tgagatcagc cactattcgc tcgtggacgt ttcgccggcc gaataccagg
     8281 tcatcgacac cctccgccaa ccccattaaa aaatctggta tcccatttgc tcaactcctg
     8341 ccggtcacct aactttcccg gcatgaaaaa aatcatttat atctttttgg cggcagcgac
     8401 cattcacacc gccagcgcac aatccgatcc caaccaccaa aacctcgccc tgcctgcccg
     8461 atccggtaac cccattttcc ccggctggta cgccgatccg gaagcagccg ccttcgatac
     8521 cgcctattgg gtcttcccga cctattccga taaatattcc aaacaggtct tcttcgacgc
     8581 tttctcctcc cccgacctcg tacaatggag caagcacccg catatcctcg acacttccgt
     8641 catccattgg gccaaaaggg cgatgtgggc gccggcaatc gcactgaaag gaggcaaata
     8701 ctatttcttt ttcgccgcca atgacatcca gaacgactcg agcctcggtg gcataggtgt
     8761 agctgtggcg cgaaaacccc agggtcccta caaagactat ctcggtaaac cactgatcgg
     8821 caagttccac aatggggctc agcccatcga tcaggccgtg tttaaagatg acgacggaca
     8881 atattatatg atctatggcg gctggaaaca ctgcaatatc gctaagctgc gtcccgattt
     8941 caaaggcttc atcccctttc cggatggcgc cacttttaag gagatcactc ccgaagatta
     9001 tgtggagggt ccgtatatga tgaagcgcgg cggcaaatat tatttcatgt ggtccgaggg
     9061 cggctggacc gggcccgatt actccgttgc atatgccatt ggagattccc ctaccggtcc
     9121 tttccgacgt attggaaaga tcctccagca ggataccacg gtcgccaccg gcgcagggca
     9181 tcattccgtg atccatattc cggggacgga cgattattat atcgtttatc acaggcgccc
     9241 gctgggggag acggacgcca accatcgggt cgtctgcatc gaccgtctcg aattcaccgc
     9301 cgatggccat atccgcccgg tcaggatcac caaagatggc gtcgccgccc gaccgcttca
     9361 gtaggctgga acggccctca cgcccgtagc cgcccgaccg cttgaatagg ccggaaaggc
     9421 atgatcaaaa tgattcctgg gcagccagat aaaaccctac cgatctgtcc gcaatccccg
     9481 catccaccac cagtctcgtc cgggattttt tatccatcat gatcctcaac cctcccccat
     9541 atcccaaacg cagataatcc agcacatgga cattcgcttc cttgttcgac gtagaagtac
     9601 agttgaggaa aatgtcgccg cccaggatac ccgtatgagc cgagatggga aaccggtatt
     9661 cgctttcccc atataacata tcttcccctc tgaatctgcc gaaagtatat cctcttcccg
     9721 tccgctgccg ctgatcataa cccaatgccg gcagcagca
//
LOCUS       DASYSV010000552        11757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1395931, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000552 DASYSV010000000
VERSION     DASYSV010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395931"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="VGQ51_17215"
     CDS             <1..1770
                     /locus_tag="VGQ51_17215"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV9038381.1"
                     /translation="GRLDIYEDRRQPLSARVDDLLKQMNPDEKAGMLFINGARVNDDG
                     SIGDKPGKGMFAFAPNALKLVTEKKMNHFNLWAVASPAALAKWYNSMQRYVQDSTRLG
                     IPITIASDPRNHFSSNVFAMTANTFSQWCEPLGFGAIGDSAFTRQFADISRREYRAVG
                     IREALHPQIDLATEPRWPRISGTFGEDAALTAKMAKAYILGYQGDQLDSNSVATMTKH
                     FPGGGPQKEGLDPHFPFQKGQVYPGHNFNYHLIPFEAAFAVHTAAIMPYYGVPMGQTS
                     EDVGMSYNKDIVTGLLRNKYHYEGVVCTDWGLVTDADLGGMVWPARAWGVEKLSREDR
                     VKKIIDAGVDQFGGENCPELVLQLLKEGRISETRIDSSVRRLLLQKFELGLFDNPYVD
                     ESKVAQEVGNPEYQKAGDASQRRAMTLLKNQGKVLPLAPGKLRIYVRNIDPKVAAAYG
                     TVVYDPKQADIAIIRLKTPFYPVESPIMMARMFHHGDLDFKGKQKDSILDLLKTVPTI
                     VDIYLDRPAVIPEINAGARGLLADFGASDQAVLDVIFGKDKPGGHLPIEMPSSMAAVR
                     AQKEDVPHDSKTPLYPFGYGLSY"
     gene            1801..3429
                     /locus_tag="VGQ51_17220"
     CDS             1801..3429
                     /locus_tag="VGQ51_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038382.1"
                     /translation="MQRRRFIRLSGMSAAALFFTSSYGGSPSPIASLQFPSRIAVLTG
                     GQRIPLSGSKERWTHRQLNVTLAHTADALSVRVHAPGMEIQQLRLSWDSPFSPACRFL
                     GDQWERSYGDLAWEAPDPARKAPWYLLVHDGHQTLAFGVKTGARTICYWDATPQQLQL
                     VLDLHSGGEGFLLGDRVLHAADIVTMQNTPGETPFHTDARFCRLMCDRPRLPVKPVYG
                     INDWYFAYGHNSKELILESTRSMTDLATDTNNRPFSVIDDGWQGEDFTVPNSKFGDMS
                     TVTSRIRDLGMRPGLWTRPLLANGKDQPSRLIPLRKGEPADSKERYIDPTIPENAHRI
                     GEVIRTHRRWGFELVKHDYSTYDLFGRWGFDMKNGLTAEGWHFNDRSKTNAEIVLDLY
                     HTIREAAGPLYLIGCNTMSNLSAGIFEINRIGDDTSGKEWARTVKMGVNTLGFRLPQH
                     NAFYAADGDCVGLTTMIPWEKNKQWMQLLAESGAPLFISAQPAATGREQREYIKTCFS
                     RSAKDQPAGEPLDWLTNPRPAKWRLNGRVVEFDW"
     gene            complement(3457..4062)
                     /locus_tag="VGQ51_17225"
     CDS             complement(3457..4062)
                     /locus_tag="VGQ51_17225"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEV9038383.1"
                     /translation="MRLIYCVVLAALLSAGHVFAQSGADTSSEHLYIDVHHLGAGKVT
                     AAAVAGAHAKDLAVEGKYGVHFLEYWVDEAGGNVYCLSSSPDTGAIRRTHAEAHGLLP
                     DEIYPVTDGTASPANAQKNYYLDVHEFPNPVSPRDVAAAHQKDLAVEGKRGVNFINYW
                     VSGNFVVCLSQAPDSNAIIETHREAHGLVPSRVIKVVPGGK"
     gene            4201..6798
                     /locus_tag="VGQ51_17230"
     CDS             4201..6798
                     /locus_tag="VGQ51_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV9038384.1"
                     /translation="MELIERAGPLTLLQSKFDAIADGEGHCVLVNGEAGIGKTALIRA
                     FCQQQKSAASIYLGTCDALFTPRPLAPLYDIIWQIWGDEGEKTVAVEDRVSLFTQFFH
                     RLGSSREPTILVFEDIHWADEATLDFIKFLARRITRIRCLFILTYRDDEVHALHPLRN
                     LLGQLPAHSFTRLQPTPLSKEAVGRLASEKGYNGRDVYTISGGNPFYVNEILASYSPG
                     IPENIKDSILSVYHRLDEKTRKLWEILSVQPTGFELNYLGQLDPQYDASLAPCLEAKI
                     IVLKEDKLLFKHELYRRTIETSLSPFLRIALHKMILGHWIHDFERVGAIYRIIHHAKN
                     ANEHELVVRFAPIAARQAAALGAHIEASRLYFSAIEYYQDGDKDRLAGFYESYAYECY
                     LTGRIEEAIIYQTRASTIWKEKGDIEKRGHTLWFLSRLWWFDGNRRQAETHAQQAIEL
                     LADRPSSSAKAMAFSNMSQLKMLSDKTVECIFWGDKAIAMAHELGDREALCHALNNVG
                     TVQMRIRGNKQKGIHLLQQSLEIALTSGFQEHAARAYTNLASDGVRIKDYPLAERILG
                     EGLRYCEERELGSWQTYMLSWKARLLLDTGKWDEAARIADDLIGKADRASIVKVGALV
                     VAGSIKMRRGEPDALVLLLEARTKAFEVMELQRILPALAALLEYEWITGQNHVEKTAI
                     EYAIGLIGQPGYLRESSEFEFWLTKVRKQQLPLHEMEKDYRASNPKMATESAAGWQRL
                     GCPYEQALLLFEGDDEGKKEAISLVHQLGAMAVYEKMKAMMRSSGITGIPRGMRKTTQ
                     ANPAQLTDRELGVLQLLKEGLQNKEIGSRLFISAKTVDHHISSILFKLDVNSRARAVQ
                     EAIRLGIVK"
     gene            6893..7171
                     /locus_tag="VGQ51_17235"
     CDS             6893..7171
                     /locus_tag="VGQ51_17235"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEV9038385.1"
                     /translation="MKKFLIERILPGAGNLSPQELQDIARASCEVVCSLGKPYHWIQS
                     FVTEDKIYCIHIAESEEAVREHSRLGKFPINTISEVKSVIDPLTSNLL"
     gene            complement(7207..7863)
                     /locus_tag="VGQ51_17240"
     CDS             complement(7207..7863)
                     /locus_tag="VGQ51_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038386.1"
                     /translation="MKKQFILILGCALCVTLCFGQGDGQGSGKSTGQGAEKSTGQGSG
                     KSTWKFRSDEYLGTVSGQMGSYGLVETVNGVYKGPWFLGLGTGLDYYRFRSVPLFFSV
                     ARDLLGAPKIGKLYVLANGGINFPWYNQAQLPYGVKSIKFYPGVWWNAGLGYRVRLSR
                     TADRAILFSVSYGMKKLSERQKGQTICPTCYLVEVQNPPTNEYEYLNRVLLFAVGFQF
                     "
     gene            complement(7860..9137)
                     /locus_tag="VGQ51_17245"
     CDS             complement(7860..9137)
                     /locus_tag="VGQ51_17245"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038387.1"
                     /translation="MRKMTSRVLLSVAAGSVLSLSGCLRDTVTESQKYTLYTPVYTLK
                     STVLASINGDPGTAIVQPGQLYIKGAFIYVNDVSKGIHVIDNSNPSHPVQTAFLNLPG
                     NTSIAVRGNTLYADMANDLLAIDITNPRQVSVTGRMSNFFPWHIYSPDTNYVLTSYII
                     RDTVVKRLVPGSGLYIVPGTIYYTLSATDVPAASNYAAQSNTSTGVAGSEASMALVGN
                     YLYSIPEPHSIDVVDISDASHPSLLTRTPVGYDLETIFPFQDKLLVGSKQGVNVYSIN
                     TANHLPVEVGTFKHGTSCDPVIADQSYAYVTLRSGTYCGGSSNELDVLDAQDITQSSL
                     LRSYPMDGPSGLGKDGSLLFVCDGSVVKVFDATDPSNLKLKTKLPVNNAYDVIASNHL
                     LIVVGSDGFYEYDYSDPTHIKLLSHLAVNRKVS"
     gene            complement(9384..9779)
                     /locus_tag="VGQ51_17250"
     CDS             complement(9384..9779)
                     /locus_tag="VGQ51_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038388.1"
                     /translation="MTATDYISGQDPARRKVLSDIHSIILKEDSTVAAEVGLMMGKEM
                     ILYKERGYFKYGLSSVKSYMSMHVMPIYGGSPLHARYQKLLPQAEFQKGCINFRNEAE
                     VPPDVLQRLFADCANVSIAALLEKRTRKK"
     gene            9841..11568
                     /locus_tag="VGQ51_17255"
     CDS             9841..11568
                     /locus_tag="VGQ51_17255"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HEV9038389.1"
                     /translation="MRSILVLMFASAFLSIQAQEPSNDLPQHPADIHRHPPNIPQHPG
                     NIHQHPPNNPQHPPDIPHLVYAHGSAELWVDGRPMLLCGGELGNSSASGMDYLRPLWT
                     KFVEMHLNTLVAPVYWDLLEPREGRFDFSLVDSLIAEARSHRLRLVLLWFGTWKNSMS
                     CYAPSWIKTDEERFPRARSGAGRPEEIVTPFSANILNADKKAFTALLQHIKETDNHRH
                     TVLMIQVENEIGMLPEARDHCPLADAAFRAPVPAMLIQYMQDHRGSLQPELRQLWEAG
                     GNKTSGGWEDIFGKSTATDEMFMAWYYGTFVEDIARAAKAVYPLPLYVNAALNAPGKL
                     PGQYPSAGPLPHVLDVWKAAAPSIDLLAPDFYNPNFRYWADRYTRADNPLFIPEHRFE
                     PGVDAKAFYAFGHYHAIGFSPFSIESTDHPADEPIGRSYDVIRQLVPMITAAAPKGGV
                     DGVLLSKEEDTSRLEMGGYRITAAHDLALGWSPKSKQDHWPLTGGIIIAVSDSVFYVG
                     GTGIVLTFQPLTQGRRAGILHAEEGRFVDGHWRPGRQMNGDQDHQGRHIRIPEGEYAI
                     QRVTLYTYK"
BASE COUNT         2709 a         3428 c         3176 g         2444 t
ORIGIN      
        1 ggccgtttgg atatctatga ggacaggcgt cagccgctat ccgcacgtgt cgacgatctg
       61 ctcaaacaaa tgaaccccga tgaaaaagcg ggcatgctct tcatcaacgg cgcccgcgtc
      121 aacgacgacg gctccatcgg tgataaaccc ggcaagggga tgttcgcctt cgcgccgaat
      181 gcgctcaagc tggtcacgga gaagaaaatg aatcatttca acctctgggc cgtcgcatct
      241 ccggcggcac tggccaaatg gtacaacagc atgcaacgat atgtacagga ctccacgcgg
      301 ctgggcatcc ccatcactat cgcgtccgat ccccgaaacc atttcagcag caacgtcttc
      361 gccatgacgg ccaacacctt ttcgcaatgg tgcgaaccgc tgggcttcgg cgccataggc
      421 gacagcgctt tcacccggca atttgccgac atctcgcgcc gcgaatacag ggcggtaggt
      481 attcgcgaag ccctccatcc ccagatcgat ctggccaccg aaccgcgctg gccccggatc
      541 agcggcactt tcggtgaaga cgccgcgctc accgcgaaga tggcgaaggc ctatatcctc
      601 ggctatcagg gcgaccagct ggactcgaac agcgttgcta ccatgaccaa acattttccg
      661 ggcggaggtc cgcaaaaaga aggtctcgat ccccacttcc ctttccagaa agggcaggtt
      721 tacccgggtc ataatttcaa ttatcatctt atcccattcg aggccgcctt tgcggtacat
      781 accgcggcca tcatgcctta ttacggagtc ccgatgggtc agacgagcga ggatgtcggc
      841 atgagctata acaaagacat tgtcaccggt cttcttcgga ataaatatca ctatgaaggc
      901 gtcgtctgca ccgactgggg tctcgtcacc gatgccgacc tgggcggaat ggtatggcct
      961 gcgcgcgcct ggggcgtgga aaagctgtcg cgtgaagacc gcgtcaaaaa gatcatcgat
     1021 gcgggcgtcg atcagttcgg cggcgagaac tgccctgagc tggtgctcca actcttaaag
     1081 gaaggtcgta tcagcgagac gcgtatcgat tccagcgtga ggcgtctgct gctgcagaaa
     1141 ttcgagctgg gtcttttcga caatccgtat gtggatgagt cgaaagtagc gcaggaggta
     1201 ggcaatcccg aataccagaa agccggcgac gcctcgcaac gacgtgctat gacccttttg
     1261 aaaaatcagg gcaaggtcct ccctctcgca cccggcaagc tcaggatcta tgtccggaac
     1321 atcgatccca aggtagcggc ggcctacggc acggtcgtct acgatccgaa acaggccgat
     1381 atcgccatca taaggctgaa gacccccttt tacccggtag agtcgccgat catgatggcg
     1441 cgcatgttcc atcatggaga tctcgatttc aaaggcaaac aaaaggatag catcctcgac
     1501 ttgctgaaga ccgttccgac catcgtcgat atctatctcg accggcccgc cgtgatcccg
     1561 gagatcaatg cgggagccag gggtctgctg gccgatttcg gagccagcga tcaggccgta
     1621 ctcgacgtca tcttcggcaa ggacaaacca ggtgggcatc ttccgatcga aatgccttcg
     1681 tcgatggcgg cggtgcgtgc gcaaaaggag gatgtgccac atgatagtaa gacgccgctg
     1741 tatccgtttg gatatggatt gagttattga agatccgggc aagttttcta ttttcgtctt
     1801 atgcaaagaa gacgattcat tcgactgtcc ggcatgtccg cggcagcact tttctttacc
     1861 agctcatacg gcggatcgcc atcgccgatc gcatcgctcc aattcccttc gcgtatcgcc
     1921 gtcctcaccg gcggacagag gataccgttg tccggttcga aagaacgctg gacccaccga
     1981 cagctgaacg taacgctggc gcacaccgca gacgctctgt ccgttcgcgt gcatgctccc
     2041 ggaatggaaa tacagcagct gagacttagt tgggactcgc ctttttcgcc tgcatgccgg
     2101 ttcctcggcg atcagtggga acgctcctat ggagacctgg catgggaagc ccccgatccc
     2161 gccagaaaag caccctggta ccttcttgtc catgacggac accagaccct ggcattcgga
     2221 gtaaaaacgg gcgcgcgaac catctgctat tgggatgcaa cgccgcagca gctgcaactc
     2281 gtcctcgatc tgcattccgg cggcgaaggc ttcctgctgg gcgaccgtgt ccttcatgcc
     2341 gcagacatcg tcactatgca aaatacgccc ggagaaactc ccttccatac ggatgccagg
     2401 ttttgcagac tcatgtgcga ccggcccaga cttccggtca aacccgtata cggcatcaac
     2461 gactggtatt ttgcctatgg acacaattcc aaagagctga tcctggaaag cacccggtcg
     2521 atgacagacc tcgcgaccga tacgaataac cggcctttct cggtgatcga cgacggatgg
     2581 cagggggagg actttaccgt ccccaattca aaattcgggg atatgtctac cgttacatcc
     2641 cggatccggg acctgggcat gcgacccgga ctttggaccc gccccctcct cgctaacgga
     2701 aaggaccagc ccagccgtct cattcctctg cgcaaaggcg aacccgcgga ttcgaaagag
     2761 cgctatatcg accccaccat tcccgagaat gcacaccgga tcggcgaggt cattcgcacg
     2821 caccggcgat ggggattcga attggtgaaa catgactatt cgacctatga cctttttggc
     2881 cgctggggtt tcgatatgaa gaacgggttg accgctgagg gttggcattt taacgaccgg
     2941 tcgaaaacga atgccgagat cgttctcgat ctctaccaca ccatccgcga ggccgcaggg
     3001 cccctctacc tcatcggctg caatacgatg agtaatctat ccgccggcat cttcgagatc
     3061 aaccgcatcg gggacgacac cagcggaaag gaatgggcac gcacggtgaa aatgggagtg
     3121 aacaccctgg gcttccgtct cccgcagcac aacgctttct atgcagccga cggcgactgc
     3181 gttggtttga ccaccatgat cccctgggaa aagaacaaac agtggatgca gctattggca
     3241 gagagcggcg cccccttatt catatcggcg cagccggcag cgacgggacg ggagcagcgc
     3301 gaatacatca aaacatgttt ctcacgatcg gcgaaggacc aacccgccgg cgaaccgctc
     3361 gactggctga caaacccgcg acccgccaaa tggcgcctga acggcagggt ggtggaattt
     3421 gactggtaaa atttggaagc caccgcctcg gcgcactcac ttccctcccg gcaccacctt
     3481 tatcaccctc gacggtacca atccatgcgc ttcccgatgc gtctcgatga ttgcattgga
     3541 atccggcgcc tgcgacagac aaaccacgaa atttccgctg acccagtaat tgataaaatt
     3601 cacgccgcgc ttgccttcca cggcaaggtc tttctgatgc gcggcggcca cgtcgcgtgg
     3661 cgagacagga ttagggaact catgcacatc gagataataa ttcttctggg cattggccgg
     3721 cgaagccgtt ccgtccgtca cgggatagat ctcatcggga agcaacccat gcgcttcggc
     3781 gtgcgttcga cgtatggcgc ccgtgtcggg tgaggatgaa agacaataca cattgccgcc
     3841 ggcttcatcg acccagtatt cgagaaaatg gacgccatat ttgccttcga ctgccagatc
     3901 tttggcgtgc gcaccggcca cggcagcagc cgttacctta ccggctccga ggtgatgcac
     3961 atctatatac aggtgctcgg aagaagtgtc cgcaccggat tgcgcgaaaa catggccggc
     4021 agaaagaagg gcggccagta caacacagta aatgagtctc atgataaatg actttaaatt
     4081 tgaaggaatg aaatgcttca caaaggaacc ttccatcctg cttttgtttg taaggataat
     4141 tccctatttt aaatgatgga acacccattt taagccatca ccacccataa tgcgcagccc
     4201 atggaattga tcgaaagagc cggtcctctc acgctgctgc aatccaaatt cgatgcgatt
     4261 gcggatgggg aagggcattg cgtcctggtg aacggagagg ccggcatcgg gaagactgcg
     4321 ctgatccggg ctttctgcca acagcaaaag tcggctgcca gcatctatct cggcacctgc
     4381 gacgccctct tcactccgcg cccgctggcc ccgctatatg atatcatctg gcagatatgg
     4441 ggcgacgaag gggaaaaaac cgtcgcggtc gaagacaggg taagcctgtt cacgcaattc
     4501 tttcatcgtc tgggcagctc ccgcgaaccg acgatcctcg ttttcgagga catccattgg
     4561 gccgacgagg ctaccctcga tttcatcaaa ttcctggcga gacgcatcac caggatacgc
     4621 tgtcttttta tactcaccta ccgggacgac gaggtccatg cgctccaccc cctgcgtaat
     4681 ctgctgggtc agctgcctgc ccattccttt acgcgcctgc aaccaacgcc attgtccaaa
     4741 gaggccgtgg gtcgtctggc ctccgaaaaa gggtacaatg gtcgggatgt atataccatt
     4801 tcgggaggca atccctttta cgtgaacgag atactggcaa gctatagccc ggggataccc
     4861 gaaaatatca aggactccat cctgtccgtc taccaccggc tcgacgaaaa gacccgaaag
     4921 ctttgggaaa tattatcggt gcagcccacc gggttcgagc tgaactatct cggccagctg
     4981 gatccgcaat atgacgcctc cctggcgccc tgtctggagg cgaagatcat cgtgttgaaa
     5041 gaggacaagc tcctgttcaa acacgagctg tacagacgta ccatcgaaac atcgctctca
     5101 cctttcctgc ggatcgcgct gcataagatg atcctcgggc attggatcca cgatttcgaa
     5161 cgcgtgggag ccatctaccg gatcatccat catgcgaaaa atgccaatga acacgagctg
     5221 gtcgttcgtt ttgcgcccat agccgcacga caggccgccg cactcggcgc acatatcgag
     5281 gcatcgaggc tctatttctc cgccatcgaa tactatcagg atggcgataa ggaccgactg
     5341 gccggctttt atgaatccta tgcgtatgag tgctacctga cgggtcgcat cgaagaggcg
     5401 atcatttacc agacacgggc ctccaccatc tggaaggaga aaggagatat cgaaaaaagg
     5461 gggcatacgc tctggttcct gtcccgtctt tggtggttcg atggcaaccg gagacaggca
     5521 gagacgcacg cccagcaggc catcgagctc ctcgctgacc gcccctcttc cagcgcgaag
     5581 gccatggcgt tcagcaatat gtcgcagttg aaaatgcttt ccgataagac ggtggaatgt
     5641 atcttctggg gcgataaggc tatcgcgatg gcacacgagc taggtgaccg ggaagccctt
     5701 tgccatgccc tgaacaatgt cggcacggtg cagatgcgga ttcggggaaa taaacaaaag
     5761 ggcatccacc tgctgcagca aagtctggaa attgcgctca ccagtggctt ccaggaacat
     5821 gccgcccggg catataccaa tctggccagc gacggggtac gcatcaagga ctatcccctc
     5881 gcggaaagga tactcgggga gggcctccgc tattgcgagg aaagagagct gggctcctgg
     5941 cagacctata tgctttcctg gaaagcaaga ctgctgctcg atacggggaa atgggacgag
     6001 gcggcacgta ttgccgatga cctgatcgga aaggctgacc gcgcctccat cgtcaaagtg
     6061 ggcgcattgg tcgtagccgg atcgataaaa atgcgacgcg gcgagcccga tgctcttgtc
     6121 ctgctgctgg aagcgaggac caaggccttc gaggtgatgg agcttcagcg gatccttccc
     6181 gcgctggccg ccctgctgga atatgaatgg atcaccggcc aaaaccacgt cgaaaagacg
     6241 gccatagaat atgccatcgg tctcatcggc caacccggct atctccggga gagcagcgag
     6301 ttcgagttct ggctgaccaa agtccgcaaa caacagctgc cacttcatga aatggagaag
     6361 gactatcgcg ccagtaaccc caaaatggca accgaatcgg cggccggctg gcaacggctg
     6421 ggctgcccat atgaacaggc cctcctgtta ttcgaaggag acgatgaggg taaaaaagaa
     6481 gcgatctccc ttgtccacca gctgggggcg atggcggttt atgaaaagat gaaagccatg
     6541 atgcgcagtt cgggcattac cggcatcccg cggggcatgc gcaagaccac gcaggccaac
     6601 cccgctcagc ttaccgaccg cgagctgggt gttttgcaat tgctcaaaga gggattgcag
     6661 aataaggaga tcggctcacg gctgttcatc tccgccaaga ccgtggatca ccatatctct
     6721 tctatcctgt tcaagctgga tgtcaattcc cgcgccaggg ccgtccagga agctatccgg
     6781 ctggggatcg taaaataggg aaccgggcct cgataaatag ggaatcctcc tgagcccgga
     6841 cccggagcct ttccctaact ttagggtaga caatcacttt aaatcgttcg tcatgaaaaa
     6901 gtttctaata gaaaggatcc tccccggagc cggaaacctt tccccacagg aattgcagga
     6961 tatcgctcgc gcctcctgcg aagtcgtttg ctcgctgggc aagccgtatc actggataca
     7021 gtcgttcgtt acggaagaca agatctactg cattcatatt gcggaaagcg aggaggcggt
     7081 ccgcgaacac tcccggttgg gaaaatttcc gatcaatacg atctcagagg taaagtcggt
     7141 gatcgacccg ctgacgagca acttgctgtg attttgcggc cgccgccggc ggcggccgcg
     7201 ctcctctcag aattgaaacc ctacggcgaa cagcaacacc ctgttcaggt attcatattc
     7261 gtttgtcggc gggttctgaa cttccacaag ataacaggta gggcagattg tctgtccttt
     7321 ctggcgctcg ctcaattttt tcataccata gctcaccgag aacagtatcg ccctatcggc
     7381 ggtgcgagaa agcctcaccc gatagcccaa tcccgcattc caccagacgc cgggataaaa
     7441 tttgatgctc ttcaccccat agggtagctg ggcctggtta taccatggga aattgatacc
     7501 gccgttcgca aggacatata gcttgccgat cttaggggcg cccagcagat cgcgcgcgac
     7561 ggaaaaaaag agcggaacgc ttcgaaagcg gtaataatcc aatcccgtcc ccaaccctag
     7621 gaaccaaggt cccttataca cgccattgac ggtctcgacc agaccatagc ttcccatctg
     7681 gccggatacc gtccccaaat attcgtcgga gcggaatttc caggtactct tcccactgcc
     7741 ctgtcccgta ctcttctccg ctccctggcc ggtactcttc ccgctgccct gcccgtcccc
     7801 ctgcccaaag cagagggtca cgcacagcgc gcaacccagg ataagtatga actgtttttt
     7861 catgatacct tccggtttac ggccagatga ctcaatagtt tgatatgggt cggatccgaa
     7921 taatcgtatt catagaaccc gtccgatccg acgacgatga gcagatgatt ggacgcgata
     7981 acgtcatagg cgttgttgac cggcagcttt gtcttcagtt tcagattgga aggatcggtg
     8041 gcgtcgaaca ccttgacgac cgaaccgtcg cagacgaaga gcagcgatcc gtccttgccc
     8101 agccccgaag gtccatccat aggataagac ctgagaagcg aggattgagt gatatcctgc
     8161 gcatccagca cgtccagctc attggacgaa cctccgcaat aggtgcccga acgcaacgtg
     8221 acataggcat atgactggtc cgcgatcacc gggtcgcagg aagtgccgtg tttgaacgtg
     8281 cccacttcca ccggcagatg attggcagta ttgatggaat agacatttac accttgcttt
     8341 gagcctacga gcagcttgtc ctgaaaaggg aatattgttt caagatcata acccacgggc
     8401 gtcctggtga gcagagaggg gtggctggcg tcggaaatat ccacgacgtc tatcgaatgg
     8461 ggctcgggaa tggaatagag gtaattgccg accagtgcca tcgaagcctc ggagcctgcg
     8521 acgcccgtcg acgtgttgct ttgtgccgca taattggacg cggccggaac gtccgtcgcg
     8581 cttagcgtgt aataaatggt gccgggaaca atatacaaac cgctgccggg aaccagcctc
     8641 ttcacgaccg tgtccctgat gatatagctt gtcaacacgt aattggtgtc cggggaataa
     8701 atatgccagg gaaagaagtt cgacatcctg cccgtgacgg agacttgccg cggattggtg
     8761 atatcgatgg ccagcagatc gtttgccata tcggcataca gcgtattgcc acggacggcg
     8821 atgctggtat ttcccggaag attgaggaaa gccgtctgga cagggtgcga tgggttgctg
     8881 ttgtcgatga catggatgcc tttgctgacg tcgttgacat aaataaaggc acctttgatg
     8941 tataactgtc ccggttgtac gatggccgtg cccggatcgc cgtttatcga ggcgaggacg
     9001 gttgatttca gggtatacac gggtgtatac agagtatact tttgagattc ggtaacggtg
     9061 tccctcagac agccggaaag tgatagcacg cttcctgccg caacgctgag aaggacccgg
     9121 ctcgtcattt ttctcatagc caggtgtttt gcagcttgac aaccggcaaa aagcgatcgt
     9181 tgcacgcact tgcaaaaatt gccgcagcag gtctagtcgt acttcctcct cgcgtcgcag
     9241 gcactgtccc gccagccgcc acctcatcat cccgcctcgc gtcgccccca tcatccctcc
     9301 agccgctgca tcatccctcc aatcaccgcc ccatcatccc tccagccgcc gcctccgcca
     9361 gccgccaccg catccccccg cccctatttc ttacgggtcc gtttttccag caatgctgca
     9421 atactcacat tggcgcagtc ggcgaatagc cgctggagga catccggagg cacctcggct
     9481 tcgttcctga aattgatgca ccccttttgg aattccgctt gcggcagcaa tttctgatag
     9541 cgggcatgca agggcgaccc gccatagatg ggcatgacat gcatggacat ataactcttc
     9601 acgctggaca acccatattt gaaatatccc ctctctttat agaggatcat ctcttttccc
     9661 atcatcaggc caacctctgc ggcgaccgtt gagtcctctt taaggatgat cgaatgaatg
     9721 tcggaaagga ccttcctgcg tgccggatct tgtccggata tatagtctgt cgcggtcatg
     9781 gtggggcggt gtttccctaa gttacttgtt ttacaggacc aaacgcggta tattcggacg
     9841 atgagatcga tcctcgtcct aatgttcgcc agtgccttcc tctccataca ggcacaggag
     9901 ccatctaacg acctcccgca gcatccggcc gacatccacc ggcatccgcc caacatccct
     9961 cagcatcccg gtaacatcca ccagcatccg cccaacaacc cgcagcatcc gcccgacatc
    10021 ccgcatttgg tctatgccca cggctccgcg gagttatggg tggacggccg tcccatgctt
    10081 ctatgcgggg gcgagttggg caattcttcc gcatccggta tggactatct gcgaccgctg
    10141 tggacgaagt tcgtcgaaat gcatctcaat acccttgtcg cgcccgtcta ctgggatctt
    10201 cttgaaccac gcgaggggcg cttcgatttc agtctcgtcg actcgctgat cgctgaggcg
    10261 aggtcacatc ggctaagact ggtgctcctc tggttcggta cctggaagaa tagcatgagc
    10321 tgctatgcgc cttcctggat aaagaccgat gaagaacgtt ttccgcgcgc ccgctccggc
    10381 gccggccggc cggaggagat tgtgacgccg ttttcagcca atatactgaa tgccgacaaa
    10441 aaggcgttta ccgccttgct tcagcatatt aaagagactg ataaccatcg gcacaccgtg
    10501 ctgatgatac aggtggagaa cgagatcggt atgctgccag aagcgcgcga tcattgcccg
    10561 ctggccgatg cggccttccg cgcccccgtt ccggcaatgc tgatccagta catgcaagat
    10621 caccgcggct cgctccagcc ggagctcagg cagttatggg aggccggcgg aaacaaaacc
    10681 tcgggcggct gggaagacat cttcggcaaa agcacggcca ccgacgagat gttcatggcc
    10741 tggtattacg ggaccttcgt cgaagacatc gcccgggcgg caaaggctgt ctatccctta
    10801 cccctatacg taaatgctgc gctcaacgcg ccgggcaagc ttccgggtca atatccaagc
    10861 gccggaccgt tgccgcacgt cctcgatgtc tggaaagcgg ctgcaccgtc catcgatctc
    10921 ctggcacccg acttctacaa cccgaacttc cgttactggg ccgaccggta tacccgcgcg
    10981 gacaacccgc tgttcattcc cgaacatcgt tttgaacccg gcgtcgacgc gaaagccttt
    11041 tatgcgttcg ggcattatca tgccatcggc ttctcgcctt tctcgatcga atccaccgac
    11101 cacccggccg atgaacccat cgggagatct tacgatgtca tccggcaact ggtgcccatg
    11161 atcaccgcgg cggcgccgaa aggcggcgtc gatggcgtat tgctgagcaa ggaggaagat
    11221 accagccgcc tggaaatggg tggatacagg atcaccgccg cccacgatct cgcgctgggg
    11281 tggtcgccga agtcgaaaca ggatcattgg ccgttgacgg ggggcatcat catagccgtg
    11341 tcggatagcg tgttttatgt cggcggcacc gggatcgtgc tgaccttcca gccattgacg
    11401 cagggccgcc gcgccggtat ccttcacgcc gaagagggtc gttttgtcga tggccattgg
    11461 cgtccgggcc ggcaaatgaa tggggatcag gaccatcagg gccggcatat ccgtatcccc
    11521 gaaggcgaat acgcaatcca gcgcgtgaca ttgtatacct ataagtaatc ccgctgcagc
    11581 cgcctggtga acgacgcgca aaaaatgggt gaaaataccc atgtcattaa gttaagccga
    11641 aattgggtga tttaacgggt cgaccgtagt tgagaatgaa gcccttaata aaaggggctg
    11701 tcccaaatca gaaacacggg cagggactac cgaatcggac taacgaaaca actcaac
//
LOCUS       DASYSV010000553         7348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2545153, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000553 DASYSV010000000
VERSION     DASYSV010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2545153"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..344)
                     /locus_tag="VGQ51_17260"
     CDS             complement(96..344)
                     /locus_tag="VGQ51_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038390.1"
                     /translation="MDTVVAIDSFYDQTPGGDYIQALKDSELYYISFHELDYLFDHFP
                     EFNFVGRMLTNKYRNPRHLTPALLMIPKKARHYRASRR"
     gene            complement(380..688)
                     /locus_tag="VGQ51_17265"
     CDS             complement(380..688)
                     /locus_tag="VGQ51_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038391.1"
                     /translation="MFLQFYLCAFFSGLFLTNAIPHFMEGISGNKFPTPFAKPPGRGL
                     SSPIVNVLWGVLNAIIGTILFVHSAIKAETTWLWVTFFVGVTLMGVLLSIGFSKKQKE
                     "
     gene            782..1630
                     /locus_tag="VGQ51_17270"
     CDS             782..1630
                     /locus_tag="VGQ51_17270"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEV9038392.1"
                     /translation="MKPLRFAIFAIAVIAGVAATPTRSHPHMVAPKVIFFDDFNTGHL
                     DRSKWNVVTTGFHVNNELQAYVDSGETLFEENGQLVFQPCYTPGFITKDGQHFDFISA
                     RINTRDKFDFQYGRAEARIRIDSGAGLWPAWWMLGYGKWPDCGEQDILEFVGERDWAS
                     AAVHGPNYFGNTPFVNRYYFPKTDDITHWHVYAVDWTPDSLNFSYDGHPMFRVTRNMT
                     GLYGPWAFDKKEFLILNFALGGAYPSKVNGIKAPYDGLPQSTVDQIRAHKVRMYVDWV
                     KVEQAP"
     gene            1627..2184
                     /locus_tag="VGQ51_17275"
     CDS             1627..2184
                     /locus_tag="VGQ51_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV9038393.1"
                     /translation="MKITLHMVSSLDGIIAKPDNSVSWFDTTDHYEKGITGEDAGAFL
                     KTIDCYVMGARTYEHALELSRSYGWAYGDVPTIVLTHRHLPVERKNVELFSGDLTELV
                     KGRLAPHYRNVWVVGGAMLAKSFLQAGLADGIRLSILPIILGDGLPFFDHIGLESPLH
                     LKEVSAYKGGMVELWYEIKDRGPNV"
     gene            complement(2211..3251)
                     /locus_tag="VGQ51_17280"
     CDS             complement(2211..3251)
                     /locus_tag="VGQ51_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038394.1"
                     /translation="MKTRLHVSLATAAAVFILLSGCVSSKKYKGSQAELARVRNDSSR
                     LAQQVSSLNGNVQDLQNKNTTLQRSLDSSSSSYANQQKSLDYYQNYFKDQQNTLSQVG
                     DDVKGALTQAGISNGDVQMMDNAVYVRLDEDELFKANSTAVKPGGKKVLDNLANVIRS
                     RQNVNVFVARGDSAGGAMAGGSDNMSSGNMSGNESGNMTEEAPAHHRTVHHRSHAMAS
                     RSSSGTATGGSQSGSSASGTNPGGSNATAQNGGSKTPVHHKVHHHYSSEGSMAIYNGP
                     GHMHNRAWALKQARMVTVANHFLQNGVAKINVSLQQPGMAGNPQDKNIRVIITPKMND
                     FTPPANRSSSMR"
     gene            3463..5343
                     /locus_tag="VGQ51_17285"
     CDS             3463..5343
                     /locus_tag="VGQ51_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV9038395.1"
                     /translation="MNKLFLLLIAACCIGFTAKAQRYFQFAHVSDTHIGGAPTAVDDL
                     RATVRDINDNPDLKFVIITGDITEFGADVELREAKTILDSLNKPWYIIPGNHDGNWSE
                     SGANSFRTVFGAETTTFQYGGFLFAGTACGPNMRMGPGQVPHEDIVWLDSVLRHLPHP
                     DMPVIYLNHYPQDSAQNNWYEAIDLLKRHNVQLVLCGHGHSNHLLNFEGIPGIMGRSN
                     LRARDTIGGYNIVTIHDDTVLYRQKRPGQPQGPVWARALLFNHHFASDTTKYYRPSFA
                     VNSEYPQVHKIWEYQGKSDIGTGNVPDGDKVIGTDTDGWIFALDRRSGKKVWGFRTGG
                     KIYSTPAVGHVGGGNAASDGYVVVASTDKNIYCLRDGRLVWKFATEKPDVACPVIAAG
                     RVYIGGSGGHFRAIDLRTGRMVWDFDQVKGFVVDKPLIYRDVIYFGCWANDFYALDKN
                     NGRLVWKWNSGASNRMFSPAACWPVATGGRVFIVAPDNVMTALDAASGKVIWRSRAPK
                     LRVRESMGLSADSSLVYVKTMEGIVIGVSTTADSLRIDWRSPVGLGYELAPTALFEKN
                     GILLVPTHSGSVWALASKDGHLLWRYKASNCMVNGILPVGQHSYVISTMDGKLTRIDV
                     RP"
     gene            complement(5400..>7348)
                     /locus_tag="VGQ51_17290"
     CDS             complement(5400..>7348)
                     /locus_tag="VGQ51_17290"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEV9038396.1"
                     /translation="GHYTGGRYFFPAVGQNGAIAAVGDEIGQVVPVWRSFSNKLMFRK
                     IDDGASDWTSHLCYLASPTVNHTSETVTAQPFSWTSDAVSPAAEADTILSTYDLYAEV
                     RYECPEFVDSCSFLKLTGTSSICDLAGGYTYRVHRNSACGEPVQWDLPPNVSLLGQTD
                     TTVTLKFPSFGTYRIASTLPFACSPIKDSLFVVAASRSHPLALGNDTTICDNTSFVLH
                     AGTGFLRYAWQDGSADSVYRVTMPGTYTVQVADSCNNLLTASVVVKPYPDTVVDAGPA
                     RVKCNTDTIQIAAPAGYLSYSWSPNYNISSLTTRQVVVDPAIDTVYVLKAGTIPGCFA
                     YDYVHIKVNHSPPITLGPDKDLCAGDSLLLDAGPGYSQYQWSTGVGTEGIAVYNKGTW
                     WVKAVTADGCLSADTLQVLNVFPLPPVDLDHSAGLCTGETRTLTAAKGYSYLWNTGST
                     AGSIAITDTGSYSVVVTDGHGCVSTDSVKVTTFLPIPTGFLPGDTAICSYGKLEITPT
                     AIFPSYLWSTGSTDESITVAKPPGVYWLEATDANGCYGTDTIVISKKDCMEGFYIPNA
                     FTPGGASNNLFRPLLFGNILRYEFGIYNRWGQLVFQSNQPGAGWDGNHQGAPAPSGVF
                     AWYCRYTLDGEPQQVQKGTVILIR"
BASE COUNT         1624 a         2007 c         2093 g         1624 t
ORIGIN      
        1 gaaaagaaga cgaattgtat ggccgcctgc aacaaagaac aggaaagacc aaggcagaga
       61 tcaagacctg gatcgacaat ctgtaaagga ttttgctatc tacgagaagc ccggtaatgc
      121 cgggcttttt ttgggatcat caataaggcc ggggtgagat gtcgaggatt ccggtatttg
      181 ttggtcagca tccggcctac aaagttaaac tccgggaaat gatcgaacag gtagtccaac
      241 tcgtggaaag aaatgtagta gagctcgctg tccttcagcg cctggatata gtccccgccc
      301 ggcgtctgat cgtaaaaact gtcgatcgcc acgacagtat ccatctccca gaaaaaccag
      361 tcggtgacct cgacgtcgtc tactcctttt gttttttgga aaacccgatg ctcaacagga
      421 cgcccatgag ggttaccccg acgaaaaaag tcacccataa ccaggtcgtc tccgccttga
      481 tggcgctgtg cacgaacagg atcgtaccga tgatggcatt gagcactccc cacaatacat
      541 tcacgatggg cgaggacaat ccacggccgg gcggtttggc aaacggcgta ggaaatttat
      601 ttccggagat gccttccata aaatgaggga tcgcgttggt gaggaacagt ccggagaaaa
      661 aagcgcaaag gtagaattgc aggaacatat atgggttttt gatatgggcc gcggcgcggc
      721 ttcccgaaga tatagcaaaa atttatgtgg attcctacga tcgtgcttat cttgccttat
      781 catgaagccg ctgcgtttcg ccatcttcgc tatcgctgtc attgctggtg ttgccgcgac
      841 acccacacgg tcgcacccgc atatggtcgc acctaaagtc atcttcttcg atgacttcaa
      901 caccgggcac ctggaccgca gcaaatggaa tgtcgtcacc accggctttc acgtcaacaa
      961 cgaactacag gcctacgtcg actcgggcga gacccttttc gaagagaacg gtcagcttgt
     1021 cttccagccc tgctacaccc ccggcttcat cacgaaagac gggcagcatt ttgattttat
     1081 ctccgcacgg ataaataccc gcgacaaatt cgatttccaa tatgggaggg cagaggcgcg
     1141 cattcgtatc gacagcggcg cgggtctgtg gcctgcctgg tggatgctgg gttatgggaa
     1201 gtggccggac tgcggcgagc aggatatcct ggagttcgtc ggcgaacgtg actgggccag
     1261 cgctgcggtc cacgggccca actatttcgg gaacacgccc ttcgtcaacc gctattattt
     1321 tccgaagaca gacgacatca ctcattggca tgtctatgcc gtagactgga cgcccgatag
     1381 cctgaatttc agctatgacg gtcacccgat gttccgggta acccgtaaca tgaccggcct
     1441 ctatggcccc tgggcattcg acaagaaaga atttctcatc ctcaatttcg cgctcggcgg
     1501 cgcctacccc tccaaggtaa atggcataaa ggcgccctat gacggtttac cccagtcgac
     1561 ggtagatcag ataagggcac ataaggtccg catgtatgtg gattgggtaa aggtggagca
     1621 agcaccatga aaataaccct ccatatggtc tcgagcctcg acgggatcat cgcaaagccg
     1681 gacaatagcg tatcctggtt cgacacgacc gaccattacg agaaaggcat caccggtgag
     1741 gatgccggcg cattcctcaa aaccatcgat tgctatgtca tgggcgcgcg gacctacgaa
     1801 cacgccctcg agctttcgag atcctacggc tgggcctatg gcgacgttcc gaccatcgtt
     1861 ctcacgcacc ggcatctacc tgtggaaaga aaaaacgtcg aattattttc gggcgacctg
     1921 acggaactgg ttaaagggcg gctggccccg cattacagga atgtctgggt cgtaggcggc
     1981 gccatgctgg ccaaatcctt ccttcaagcc ggcctcgcgg acggcattcg cctgtccatc
     2041 ctgcccatca tactcggtga cgggcttccc tttttcgacc acatcggcct cgaatcgcct
     2101 ctccatctca aagaggtgtc ggcctataaa ggcggcatgg tggagttgtg gtatgagata
     2161 aaagatcggg gaccgaacgt gtgaatccgg ccgatccccg atatctccga tcagcgcatc
     2221 gacgaggaac ggtttgccgg cggagtgaaa tcgttcatct tcggagtaat gatcacccgg
     2281 atattcttat cctgcggatt gccggccata ccgggctgct gcaacgatac attgatcttc
     2341 gcaacgccat tttgcaggaa atgattggcg accgtcacca tgcgggcctg tttcaatgcc
     2401 caggcacggt tgtgcatgtg gccgggacca ttataaatgg ccatcgaacc ctcggaagaa
     2461 taatggtgat gtactttatg gtgtaccggg gtcttcgatc cgccattctg cgcggtggca
     2521 ttcgagccgc cggggttggt gccgctggcc gaagagccgc tttgtgaacc gccggtggct
     2581 gtcccggacg agctccggct agccatagca tgactcctgt gatgcaccgt cctgtgatgc
     2641 gcaggcgctt cttcagtcat attaccactt tcgttgccgg acatattacc ggaagacatg
     2701 ttatcggagc ctccggccat agccccaccc gccgaatcac cacgcgcgac gaatacgttg
     2761 acattttgcc tgctcctgat cacgttggct agattgtcca gtaccttctt gccgccgggc
     2821 ttaacggctg tgctgttggc tttgaagagc tcatcctcat ccagccgcac atagaccgcg
     2881 ttgtccatca tctggacatc gccgttggag atccccgcct gcgtcagggc gcctttcacg
     2941 tcatcgccaa cctgcgacaa tgtgttttgc tggtccttga aataattttg ataataatcg
     3001 agcgactttt gctggttggc atagctgctg gagctgctgt cgagtgatcg ttgcagcgtc
     3061 gtgttcttgt tctgcagatc ctgcacgttg ccattcagcg aacttacctg ctgcgcaaga
     3121 cgcgagctgt catttcttac tcttgccagt tcggcctggg atcctttgta tttcttactg
     3181 gatacgcatc ccgacaacaa aataaatacg gctgctgccg tagcgagcga cacatgtagt
     3241 ctcgttttca taaacatggt gttttaatct aatgtctacc taaattttta tgccacggaa
     3301 gagggcatga tacggacgcc ggaaaagctc tggatgagga ccggcgtccc cgtatgtccc
     3361 ggaggttacc cagaatttcg atatacgcgt gaccccggga cgccggcgga cccgcacccg
     3421 gttttgtttt tttgcgctcc agagccgaac ttgcgcacca acatgaacaa gctattccta
     3481 ctcctcatcg cggcctgctg catcggattc acggctaagg cacaacgtta tttccaattc
     3541 gcacacgtat cggataccca tatcggcggg gcgcctaccg ccgtcgacga tcttcgcgcg
     3601 acggtccggg acatcaacga caacccggac ctcaaattcg tcattatcac tggcgacatc
     3661 acggaattcg gcgccgacgt ggagctgcgc gaggcaaaga cgatactcga cagtctcaac
     3721 aaaccctggt acatcatccc cggcaatcat gacggcaact ggagcgagag cggagcgaat
     3781 tccttcagga cggtgttcgg tgccgagacg acgacctttc aatacggcgg attcctgttc
     3841 gcggggacgg cctgcggtcc caatatgcgt atgggcccag gccaggtacc ccatgaagac
     3901 atcgtgtggc tcgactcggt gctgcggcat ctgccgcatc cggatatgcc tgtcatttat
     3961 ctcaaccact acccgcagga ctccgcgcag aataattggt acgaggcgat cgatctgcta
     4021 aaacggcaca atgtccaact ggtgctctgc ggccacggtc attccaacca tctgctgaat
     4081 ttcgagggca ttccgggtat catgggacgc agcaatcttc gggcgcgaga caccatcgga
     4141 ggatacaata tcgtgacgat ccatgacgac accgtgcttt accggcaaaa aagaccgggt
     4201 cagccgcaag gaccagtctg ggcgagggca ttgcttttca atcaccattt cgcgtcggac
     4261 acgacaaagt attatcgtcc gtcgtttgcc gtcaattcgg agtacccgca ggtgcataaa
     4321 atatgggaat accaggggaa gagtgacatc ggtaccggga atgtccccga tggcgacaag
     4381 gtcataggta ccgacaccga tggatggata ttcgcgctgg accggcgcag cggaaaaaag
     4441 gtttggggat tccgaacagg cggaaagatc tattcgacgc cggcggtggg gcatgttggg
     4501 ggcggcaatg cggcgtccga cggctacgtc gtggtcgcct ccacggataa aaatatttat
     4561 tgtctgaggg acggccggct cgtctggaag tttgcgacgg agaaacccga cgttgcatgc
     4621 cccgtaatcg cggcagggag ggtctatatt ggcggatcgg gcggtcattt cagggcgatc
     4681 gacctgagga cgggtcgcat ggtatgggat ttcgaccagg tgaagggttt cgtggtcgac
     4741 aagcccctca tctatcgcga cgtcatctat ttcggatgct gggccaatga cttttatgca
     4801 ttggataaaa ataatggccg gctggtctgg aaatggaata gcggcgcctc taatcgaatg
     4861 ttctcgcctg cagcctgctg gcctgtggct acgggaggcc gtgtgttcat cgtcgccccg
     4921 gacaacgtga tgacggccct ggatgcggca tcgggaaagg tgatctggcg gtcgagggcg
     4981 ccgaagctgc gcgtgcgcga gtcgatgggt ctatcggcgg acagctcgct tgtctatgtc
     5041 aaaacgatgg aaggtattgt tattggcgtc tcgacgactg ccgactcgct gcggatcgac
     5101 tggagatcgc cggtcggact tggttacgag ctggcgccta cggcgctctt cgagaagaac
     5161 ggcatactgc tggtacctac ccattcaggt tctgtgtggg cgctggccag caaggatggc
     5221 cacttattgt ggcgctacaa ggcatcgaac tgcatggtta acggtattct gccggtgggg
     5281 cagcatagct atgtgatcag cacgatggat gggaagctta cccgcatcga cgtcaggccc
     5341 tagccggcgg ccgtgaattc atcgccggat gttcacgcgt aaccggcggc cgcgcactgt
     5401 taccgtatca gaataaccgt acccttctgc acctgctgcg gctcaccatc cagcgtatac
     5461 cggcaatacc acgcaaacac tccggaaggt gccggtgcac cctgatgatt tccatcccag
     5521 ccagcgccgg gctgattgct ttggaagacc aactgacccc atcggttgta aatgccgaat
     5581 tcatatcgga ggatattgcc gaacagcagc ggtctgaaca ggttgttgct ggcgccgccg
     5641 ggagtgaagg cattcgggat atagaagcct tccatgcaat cttttttcga aatgacgatc
     5701 gtgtccgtcc catagcagcc gttggcatcg gtggcttcca gccagtatac ccccggtggt
     5761 ttggcaacgg tgatcgattc atcggtggag cccgtgctcc agaggtaaga cgggaagata
     5821 gccgtcggcg ttatttccag cttgccatat gaacatatgg ccgtatcgcc cggcagaaat
     5881 ccggtcggga tcggcaggaa ggtcgtgacc tttaccgagt cagtactcac acatccatgg
     5941 ccatcggtta cgacgacgga atagctgccc gtatcggtta tagcgatgct tccggcggtg
     6001 cttccggtgt tccagagata gctgtagcct ttcgctgcgg tcagtgtccg ggtttcgccg
     6061 gtgcaaagtc ccgcactatg atcgagatcg acgggtggca ggggaaagac gttcagcacc
     6121 tgcagcgtat cggccgaaag acatccatcg gcggtcacag cctttaccca ccaggtgcct
     6181 ttattgtaaa cggcgatccc ttcggtaccc acgcccgtgc tccattgata ttgtgagtat
     6241 cccggtccgg catccaataa caggctgtcc ccggcacaga ggtccttgtc cgggcccagc
     6301 gttatcggcg gggaatgatt gactttgata tgcacgtagt catatgcgaa acaacccggg
     6361 atcgtgcctg ccttcagaac atataccgtg tcgatcgccg gatcgacgac cacttgtcgg
     6421 gtcgtcagcg agctgatatt gtaattcggc gaccagctat agcttagata gccggcaggc
     6481 gccgcgattt gaatggtgtc ggtattgcat ttgacacgcg cgggtccggc gtcgaccacc
     6541 gtatccggat atggctttac gaccaccgag gcggtcagga gattgttgca ggagtcggca
     6601 acctgtacgg tataggtccc cggcatcgta accctgtata ccgagtcggc gcttccatct
     6661 tgccatgcat aacgcaggaa accggttccc gcatgcagga cgaaagaggt attgtcgcag
     6721 atggtcgtat cgttgcccag cgctaacggg tgcgagcggg aggccgcgac gacgaacagc
     6781 gagtctttga tgggagaaca ggcgaagggt aatgtggagg cgatccggta ggtgccgaaa
     6841 gaaggaaatt tcagcgtgac ggtagtatcc gtctgaccga gaaggctgac atttggcgga
     6901 agatcccatt gcacgggttc gccgcaggcg ctgttgcgat gcacgcggta ggtgtatccc
     6961 cccgcgagat cgcagatgga gctggtgccg gtgagcttca gaaaactaca ggaatctacg
     7021 aattccgggc actcataacg gacctccgcg tagaggtcat aggtgctcaa aatagtgtcg
     7081 gcctcggccg ccgggctcac ggcatccgag gtccaggaga acggttgcgc cgtgacggtc
     7141 tcggaggtat ggttgacggt gggactggcg agataacata aatgggaggt ccagtcactg
     7201 gcaccgtcgt cgatctttct gaacatgagt ttattggaga aggatcgcca gaccgggacc
     7261 acctgaccga tctcgtcgcc gacagctgct atcgcaccat tctggcccac cgccgggaag
     7321 aagtaacgtc ctcccgtgta atgaccat
//
LOCUS       DASYSV010000554         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1395958, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000554 DASYSV010000000
VERSION     DASYSV010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395958"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VGQ51_17295"
     CDS             <1..944
                     /locus_tag="VGQ51_17295"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV9038397.1"
                     /translation="STLMGSPSSDAFVFACIPIIGYFVSIWIRANREDKRGIGALLYM
                     FAVSVIFWALYYQIFTGYTLWTDKHTDRTISSAVVRNGASSLGLMQTVNTSPRPVDSV
                     DAELEPVVVNGHALKTMGPDPYFNNLPKDQWPPEGKGLEVANPELYTSIVPFYIVTLT
                     PLLVGLFTWLRRRGKEPTIPGKFGIALLLSGVSSLVMYFAVISVASIYHFKVSPGWLW
                     GTYFVICCAEICLSPMGLSLVSKLAPPRLTSLLMGGWFLTMALGSKIAGVMASSWGTF
                     PDKRIYFIIWFIAGVIGSAFIFSRIRSLSRIVSEKTG"
     gene            947..>2197
                     /locus_tag="VGQ51_17300"
     CDS             947..>2197
                     /locus_tag="VGQ51_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEV9038398.1"
                     /translation="MTDVKFKPFVSPETEMKEFTFKALLAGSIFGILFGASTVYLALK
                     AGLTISASIPIAVLAITLGRRVFKTTILENNIIQTTGSASESIAAGIVFTLPGFLFMS
                     TAQSADFFNYGVIFIVAIFGGFLGVLLMIPLRRSLIVKEHETLPYPEGTACGAVLKAG
                     EKGGEFAKAAFQGLGFAAVYALGQKLFRFIAETPLFDSVKSKGLSSIFPAAQVSADIT
                     PEYMGVGYIVGPKIAGILVAGSVLSWFVMHPLLASLVDPVTIANQQVKLGLLNDIHTA
                     GGAGAWNPVTRTFADPATALYRAYIRLIGAGAVAMGGLITLIKTIPTIVASFSESVGS
                     VKEAGKESRIRTERDLNIKIVAFGSLVLVVLIGCLPIIPGDSFFGRLLIGVLILVFGA
                     FFVTVASRIVGIIGSSNSPVSGMTI"
BASE COUNT          434 a          617 c          622 g          524 t
ORIGIN      
        1 atagtacctt gatgggctcg ccatcgagcg atgcctttgt gttcgcctgt atccccatca
       61 tcggttattt cgtctccatc tggataaggg ccaaccggga ggacaaacgg ggcatcggcg
      121 ccctccttta tatgttcgcc gtcagcgtta tcttctgggc gctgtattat caaatcttca
      181 cgggttatac gttgtggaca gacaagcaca cggatcgcac gatcagttcg gccgtcgtac
      241 gcaacggagc cagttcgctg ggtctgatgc aaacggtcaa taccagtccc cgaccggtgg
      301 actccgtgga cgccgagctg gagccggtgg tggtgaacgg ccatgcactg aagaccatgg
      361 gacccgaccc ttacttcaac aatctgccaa aagaccaatg gccgccggaa ggaaagggtc
      421 tcgaggtagc caatcccgag ttatatacct ccatagtacc gttttatatc gtgacgctga
      481 caccgctgct ggtaggactg ttcacctggc tgcgacgacg cggcaaggaa ccgacgatac
      541 ccggcaaatt cgggatagcg ttgcttcttt ccggggtatc ctcgctggtg atgtactttg
      601 ccgtgatctc cgtcgcatcc atttaccatt tcaaagtatc gcctggctgg ttatggggta
      661 cttatttcgt catctgctgc gcggagatct gtctgagtcc gatgggtctg tcgctggtgt
      721 ccaaactggc cccgccacga ctgacctccc tgttaatggg cggttggttc ctgaccatgg
      781 cgttaggcag caagatcgcg ggagttatgg caagctcctg gggcacattc cccgataaac
      841 gcatttattt tatcatctgg ttcatcgcgg gcgttatcgg ctcggccttt atcttcagcc
      901 gtatccggtc gttgagccgt atcgtgagtg aaaaaacagg ctaagcatga cagacgtaaa
      961 attcaagcct tttgtcagcc cggaaacgga gatgaaagaa tttaccttca aggccttgct
     1021 ggcgggaagt atcttcggca tcctgttcgg cgcctcgacc gtctacctgg cgctgaaagc
     1081 cgggctgacc atctcggcct cgatccccat cgccgtcctc gccatcacgc tgggccgacg
     1141 ggtatttaaa acgacgatcc tggagaacaa tatcatccag acgacgggca gcgccagcga
     1201 gagcattgcg gcgggcatcg tcttcacgct gccgggcttc ctgttcatgt cgacggcgca
     1261 atcggccgac tttttcaact atggcgtcat ctttatcgtc gccattttcg gcggatttct
     1321 gggggtgttg ctgatgatac cgcttcggcg gtcgctgatc gtcaaagaac atgaaacact
     1381 gccttatccg gaaggcacgg catgcggcgc ggtgctgaag gcgggtgaaa aaggcggcga
     1441 attcgccaaa gctgcattcc agggtcttgg ctttgccgct gtgtatgccc tcggtcaaaa
     1501 acttttccgg ttcatcgccg agacgccgct attcgattcg gtgaaaagca aggggctcag
     1561 ttccatcttc ccggcggcgc aggtctccgc ggacatcacg ccggaatata tgggggtcgg
     1621 ctatatcgtc ggtccgaaga tcgccggcat tctcgtggcc ggcagcgtcc tgagctggtt
     1681 cgtgatgcat cccttgctgg catcgctggt cgacccggtg acgatcgcca accaacaggt
     1741 aaagctgggt ttgctgaacg acatccatac cgcgggaggc gcgggggcct ggaacccggt
     1801 gaccaggaca ttcgcggacc cggccactgc cctttaccgg gcctatatcc ggctcatcgg
     1861 agcaggagcg gtcgccatgg gaggcctgat cacgctgata aaaaccattc caacgatcgt
     1921 tgcttctttt tcggagagcg ttggctcggt aaaagaggcc ggaaaagaaa gccgtatccg
     1981 gaccgaacgc gacctgaata tcaagatagt ggcttttggc agtctcgttc ttgtcgtcct
     2041 cattggctgt cttcccatca ttcccggtga ctccttcttc ggccggcttc tcatcggtgt
     2101 cctgatcctg gtattcggtg ctttcttcgt gacggtagcc agccggatcg taggcatcat
     2161 cggttcatcg aatagcccgg tatccggaat gaccatt
//
LOCUS       DASYSV010000555         9076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_884485, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000555 DASYSV010000000
VERSION     DASYSV010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884485"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /locus_tag="VGQ51_17305"
     CDS             complement(<1..1867)
                     /locus_tag="VGQ51_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038399.1"
                     /translation="MIKNYLRVAFRNLWRHKGFSFLNILGLTIGMSACFLIFLYVKFE
                     LSYDHFHSKADRIYNIVTDINNPTEVLHFSVEAPAMSVAAKRDFPEIEKQVRFSNGNI
                     LVRRGDLKIQEDNMAFADSTFFEIFDFPLLKGNPVTALRGPLTVVLSETAAKKYFGTA
                     DPMGQHLQVFNNGQLATVTGVMKDMPENTELKTEMLMSMYNGPQDSSRDQNWGGFGVM
                     AYFLLKPHTSAQALANKFPAFLQNHIGKFMKENNQTYTYILEPLKDVYLKSTPGNAQG
                     SLTNIYVFSIVGVFILLIAGINFVNLTTARSTERAREVGIRKVIGAERRQLTRQFLGE
                     SVILCLIAFVLSVGACAALLPSFNFLAGKTISTGIFHHPDYILDLLLIGLGIGLLAGI
                     YPALVLSAFRPIIVLKGRFATGVRGLLLRKGLVISQFTISIGLIAATLLVGYQLSYMR
                     QQDLGFAKEQELVMDTHGDDHKIALKEEIRHLPGVLSAAMSSSTPGTGAMNAYSMLQN
                     QKGAMQICSPDLFWVDFDFIPQYQMKVVAGRAFSRAFGTDTTHSMILNERAVKMLGYH
                     SPQDAIGRDFNQWGRKGKIIGVVKDFHYEGLQQEIRPLSLRIEPGGCTMLSVKVST"
     gene            complement(1952..4342)
                     /locus_tag="VGQ51_17310"
     CDS             complement(1952..4342)
                     /locus_tag="VGQ51_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038400.1"
                     /translation="MFRNYLRTAFRNLAKHWGFSLLNVIGLTVGMVAFFLIFLYVRFE
                     LSYDTMHSKGDRIYRIICNLQTPTETLTLNQPSWAVAPHLVGQLPEIEAAVRVSQGDN
                     WLVTQGDEHFQQEDVAMVDSNFLQVFDFPLLKGNRATALKEPNSVVLTETTAKKFFGN
                     ANPMGKPLLLTRGKMHATVTGVLKDIPDNSQIKASMLVSISTVTRNNGGGRSIDDQWG
                     NYGPQAYVLLKPGTSAAQLQKKFPAWLEKMNGKEMHESHMIPTLLLEKLRGIYLYSDR
                     DDSKTGHITNVYIFSIIGAFILIIACINFINLTTARSAERAKEVGIRKVIGALRPQLA
                     RQFIGESVILCLISYLLAILLAALLIHPFNQLAGKIVSPGIFARPVDLLILLGIAVLI
                     GIAAGIYPALVLSSFQPAAVLKGRFASGTRGIVLRKTLVIVQFTIATALIIGTLVVWQ
                     QLSFMRSQDLGFNQTQKMVLDTRGDSAKLTLKQEVLRIPGVLASAMSSSVPGTGNPGA
                     YSQIQNRRGDMQVANLDLYFVDFDYIPVLKMKMIAGRAFSRDFMTDTTHSMILNEAAV
                     REFGYTSPEQALGRQFDQWGRKGTIVGVVKDFHFHGLQEVIKPLSMRIEPQGCDLLIV
                     NVEGHNIPNTVAAIERLWKQMLPDKPFSYFFLDEHFDTQYRAEDRFGRLFLYFAFLAI
                     LISCLGLMGLASYSTVQRTKEIGVRKIVGASVSSIVVLLSKDFLILVGISLLIAGPAA
                     YYFLHGWLQGFAYRIPIYWWIFVSASITALLVALLTVSFQAVRAAMANPVESLRSE"
     gene            complement(4417..4860)
                     /locus_tag="VGQ51_17315"
     CDS             complement(4417..4860)
                     /locus_tag="VGQ51_17315"
                     /inference="COORDINATES: protein motif:HMM:NF025491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HEV9038401.1"
                     /translation="MLQRKQTVWTLLAAICAALTFNYSFYHGQVPVGQYGHEIRFLTA
                     WPHFVNGTSGSILITIVTIVLVAGALWNIFNYKNRTRQFWVTIGLIVISFLNIFLYWN
                     SLSEFQEGGLSLSAVFVLLIPVLLYFAARGIRKDEKLVKSADRLR"
     gene            complement(4871..6160)
                     /gene="glyA"
                     /locus_tag="VGQ51_17320"
     CDS             complement(4871..6160)
                     /gene="glyA"
                     /locus_tag="VGQ51_17320"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV9038402.1"
                     /translation="MQKDTLVFDLIEKELERQRHGIELIASENFTSQAVMQAMGTVLT
                     NKYAEGYPGRRYYGGCEIVDQVEQLAIDRLKQIFSCDYANVQPHSGAQANAALMLAIL
                     QPGDTILGLDLSMGGHLTHGSPVNFSGKLYRPVSYGVGREDGLVDYNMMEVVAKKERP
                     KLIICGASAYSRDWDYARIRSIADSVGAFVMCDMAHPAGLIAKGLLNSPFEHCHFVTS
                     TTHKTLRGPRGGIILMKKDFDNPFGLKDVKGNIRPMSNLIDMAVFPGTQGGPLEHVIA
                     AKAVAFGEILSNDFTVYAKQIISNAQAMAKAFLAKGYHIVSGGTDNHLLLIDLRNKNI
                     SGKKAENTLGKAEITVNKNMVPYDDKSAFITSGIRVGVPAITTRGMREVDMITVVEMI
                     DRALTNAENDDVLNALRSEVNIFMKQFPLYEELPQLV"
     gene            6248..7246
                     /locus_tag="VGQ51_17325"
     CDS             6248..7246
                     /locus_tag="VGQ51_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEV9038403.1"
                     /translation="MKAIIPVAGAGTKLRPHTYTQPKALIPLAGRTILSIIVDQLTEA
                     GITEFIFIVGYLGEKIQDFVKQKYPHLTAHFVYQNERQGIGHAVLLTRHIVGDDELFI
                     VLGDTICEYDVKDVLNQPHSLLGVKRVDDPRVFGVAEMGDDGIIERVIEKPQIPKSNM
                     ALVGIYRIRESGVLFKCLETNIQNHVTSYGEYSLTDAIECMIRNDVKFRAFKVQNWFD
                     CGKKETLLESNAKLLKKFGGTIAPEHQFENTIIIQPVSIAPGCDIRNSIVGPNVAIGE
                     KTRVNYSILKDSIIGSFADLYDIVLTHSLIGSDTEVKGESRSLNIGDNTEIDLGES"
     gene            7478..7879
                     /locus_tag="VGQ51_17330"
     CDS             7478..7879
                     /locus_tag="VGQ51_17330"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV9038404.1"
                     /translation="MGTPKKLLFVLLLPLALYAKAGTGKLEGVLNGYVTDAVTKKPLS
                     GVVVSAFIPGTSLLKEVLTDADGYFRFAELPSSQVTVEFNKKGYQLARRPNVAIKEKT
                     SVKLNVEFLPDDPDSGLGGGEYPLLRMLEAN"
     gene            complement(8247..>9076)
                     /locus_tag="VGQ51_17335"
     CDS             complement(8247..>9076)
                     /locus_tag="VGQ51_17335"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038405.1"
                     /translation="GKEAKASNQTSSYVIGYVSGFLIYLLTFIYGAMVMRGVMEEKTN
                     RIAEVVVSSVRPFQLMMGKITGIGAVGLTQFLLWVILIGGLAAVAQGFVSHDTWQQVQ
                     QAQQAGAVGNASAAQASEKAQLLPKVLGALSTANLWLILGCFLFYFIGGYLFYSSLFA
                     AVGSAVNEDPQEAQSLMLPITMPVIFSFIIMSVAVQDTSGALAVWASIIPFSSPIVMM
                     ARIPFGVPAWQLGLSMLFLILGFLFTVWLAGKIYRTGILMYGKKPTWKIMWKWAFHK"
BASE COUNT         2066 a         2515 c         2475 g         2020 t
ORIGIN      
        1 tggtgctcac tttcaccgag agcatagtac aaccgccggg ctcgatccgc aggctcagcg
       61 gcctgatctc ctgctgcaaa ccctcgtagt gaaagtcttt gaccaccccg atgatcttgc
      121 ctttgcgtcc ccattgatta aaatcccgtc cgatcgcgtc ctgcggactg tgataaccca
      181 gcatcttcac cgcccgctcg ttcaggatca tggaatgcgt ggtatccgtc ccgaaggccc
      241 gtgaaaaagc cctgcccgcg accaccttca tctggtattg cggtataaag tcgaaatcga
      301 cccagaaaag gtcgggtgaa cagatctgca tcgcaccttt ttggttctga agcatcgaat
      361 acgcattcat ggctcccgtc ccgggcgtgc tggaggacat tgccgcggaa agcacgccgg
      421 gcaggtgtct gatctcctcc ttgagcgcta tcttatggtc gtccccatgc gtgtccatga
      481 ccagctcctg ttccttggcg aacccaaggt cctgctgccg catatagctt aattgatagc
      541 ccacgagcag ggtggcggcg atgagcccga tcgagatggt gaactgcgag atcaccagcc
      601 cctttcggag cagcagcccg cgaacgccgg tggcaaaacg tcccttcagg acgattatcg
      661 gccggaaggc cgacaggacc agtgcgggat agatgcccgc cagcaacccg atgccaagac
      721 cgatcagcag cagatccagg atatagtcgg gatgatggaa gatgcctgta ctgatcgtct
      781 ttcccgccag gaagttgaag ctgggtaaca gtgccgcgca agcgccgacg gacagcacaa
      841 aggcgatcag acacaggatc accgattccc cgaggaactg ccgggtcagc tgtcgccgtt
      901 cggcgccgat caccttccgg atgcctactt cgcgcgccct ctccgtcgac cgcgccgtcg
      961 tcaggttgac gaaattgatc cctgcgatca gcaggatgaa gacacccacg atgctgaata
     1021 catagatatt cgtcagactg ccctgggcgt tcccgggtgt cgatttcaga tacacatcct
     1081 tcagcggctc gaggatatag gtataggtct ggttgttctc cttcatgaac ttaccgatgt
     1141 ggttctggag gaacgcgggg aatttgttag ccagcgcctg cgcgctggtg tgcggtttca
     1201 gcaggaaata ggccatgacc ccaaaaccgc cccagttctg atccctcgag gaatcttgag
     1261 gaccattgta catcgacatc agcatttccg ttttcagttc ggtattctcc ggcatatctt
     1321 tcatgacgcc cgtgacagtc gccagttgac cgttgttaaa aacctgcagg tgctgtccca
     1381 tcggatcggc cgtcccgaaa tatttttttg ccgcggtctc ggaaagcacc acggtcaacg
     1441 gtccacgcaa tgctgtgacg gggttgcctt tcagcaaggg gaaatcgaag atctcgaaga
     1501 acgtcgaatc cgcaaaagcc atattatcct cctggatctt aagatcgccc cgccggacca
     1561 ggatgttgcc gttgctgaac cgtacctgct tctcgatctc cgggaaatcc cgtttggctg
     1621 ccaccgacat cgccggcgct tcgacgctga aatgcagaac ttcggtcgga ttgttgatat
     1681 ccgttacgat gttgtaaatg cgatcggcct tcgaatggaa atgatcgtag ctgagctcga
     1741 acttcacata caggaagatg aggaaacagg ccgacatccc gatggtcaga cccaggatgt
     1801 tcaggaagga gaagcccttg tggcgccaga ggttgcgaaa ggcgacgcgg agataatttt
     1861 ttatcataca gttggtttgt taactctcca ccggattggc catcgccatt tattcactgc
     1921 gcaaactctc cacgggattg ggcatcgcca tttattcact gcgcaaactc tccactggat
     1981 tggccatcgc cgcgcgcacc gcctggaagc tcaccgtcag caaagccacc agcaatgcgg
     2041 ttatcgacgc cgacacgaag atccaccagt agatgggtat ccggtaagcg aacccttgca
     2101 gccatccatg caggaaataa tatgccgccg gaccggcgat cagcaaggaa atccctacca
     2161 gtatcaggaa gtctttagac aacagcacga cgatgctgct caccgacgcg ccgacgatct
     2221 tccggacccc gatctctttc gtcctctgca ccgtgctata ggaagccagc cccatgagcc
     2281 cgagacagct gatcaggatc gcgaggaacg cgaaatacag gaagagccgg ccaaaccggt
     2341 cttcggcccg gtattgcgtg tcgaaatgtt cgtcgagaaa gaaatagctg aacggcttat
     2401 cgggcagcat ctgtttccac agccgctcga tcgcggcgac ggtgttcgga atattatgcc
     2461 cttcgacatt gacgataagc agatcgcagc cttgcggttc gatgcgcatg ctcagcggct
     2521 tgatcacctc ctgcagaccg tggaaatgga agtctttcac caccccgacg atggtcccct
     2581 tgcgccccca ttgatcgaat tgtcttccca gcgcctgttc cggcgaagta tagccgaact
     2641 ccctgactgc cgcttcattg aggatcatag aatgcgtcgt atcggtcatg aagtcgcgcg
     2701 agaaggcacg gccggcgatc attttcattt tcaacacggg gatataatcg aaatctacga
     2761 agtataggtc caggttggcg acttgcatgt cgcctctacg gttttggatc tgcgaatagg
     2821 cgccggggtt gcccgtcccg gggacactgc tcgacattgc gcttgccagc acgccgggta
     2881 ttcgcaacac ctcctgtttc agcgtcagtt tggccgagtc accccgtgta tcgaggacca
     2941 ttttctgtgt ctggttgaaa ccgaggtcct ggctcctcat aaagctcagc tgttgccata
     3001 ctaccagcgt gccgatgatc agcgccgtcg cgatcgtaaa ctgtacgatg accagcgtct
     3061 tccgcaacac gatacctctg gtacccgacg cgaatctccc tttcaggacc gccgccggtt
     3121 ggaaggacga caggaccagc gccgggtaga tgccagcggc gatgccgatc agcacggcga
     3181 tacccagcaa tatcagcagg tcgacgggac gggcgaagat acccgggctc acgatcttgc
     3241 ccgccagctg gttgaaagga tggatcagca gcgcggccag caggatcgcc agcagatagg
     3301 agatcagaca aaggatgacg ctctcgccga tgaactgacg cgccaattgc ggtctcaggg
     3361 cgccgatcac cttccggatg cctacttcct tggccctctc cgccgaccgc gcggtggtga
     3421 gattgatgaa gttgatacag gcgatgatga ggataaaggc ccctatgatc gaaaagatat
     3481 agacattggt gatatgaccc gtcttggagt cgtctctgtc gctatagaga tagatacctc
     3541 tcagcttttc gagcagcagg gtcgggatca tgtggctctc gtgcatttct ttcccgttca
     3601 tcttttcgag ccaggcgggg aattttttct gaagctgcgc ggcactggtg cccggtttca
     3661 gcaacacata ggcctgcggg ccgtagttgc cccattggtc gtctatactc cggccgccgc
     3721 cattgttacg cgtgacggtg ctgatggaaa cgagcatgct cgccttgatc tgtgaattgt
     3781 cgggtatgtc cttcagaacg cccgtgaccg tggcgtgcat tttaccccgg gtcagcaata
     3841 acggcttgcc catcgggttg gcgttcccga agaatttctt ggcggtggtc tcggtcagca
     3901 ctacactgtt cggctccttc agcgcggtgg cccggttgcc tttcagcaag gggaaatcga
     3961 agacctggag gaaattcgaa tccaccatcg ccacgtcctc ctgctggaaa tgctcgtctc
     4021 cctgcgtgac cagccagttg tccccttgcg agaccctgac ggcggcctcg atctccggca
     4081 gctgtcccac caggtggggc gcgacggccc aggaaggctg attgagagtg agcgtctcgg
     4141 tcggcgtctg gagattgcag atgatgcggt agatgcggtc acccttgcta tgcatggtgt
     4201 catagctgag ctcaaaccgg acatagagga agatcaggaa aaaggccacc atcccgacgg
     4261 tgagcccgat gacgttaagc agtgaaaaac cccagtgttt cgcgagattg cggaacgccg
     4321 tgcggagata gtttcggaac ataaaaggga aattagactc ttaaagtacc gaaatccccg
     4381 cggatgttgc atcccgcgcc gccggaagca cgaaatttaa cgtaaccggt cggcgctttt
     4441 aactaatttt tcgtctttcc ggatgccgcg ggcggcgaag tacaacaaaa cggggatcag
     4501 caggacgaaa acggccgaaa gactgagccc tccctcctga aattcactga gagaattcca
     4561 ataaagaaag atattgagga aggagatgac gatcagcccg atggtcaccc agaattggcg
     4621 ggtcctgttc ttgtaattga agatattcca gagggccccg gcgaccagga caatggtgac
     4681 gatggttatc agaatgctcc cgctggtccc attgacaaaa tggggccagg ccgtgaggaa
     4741 gcggatctcg tggccatact ggccgaccgg gacttgccca tgataaaagg aataattgaa
     4801 ggtcagggca gcgcatatgg cggccagcag ggtccacacg gtttgttttc gttgcagcat
     4861 aaaagacgat ttataccaat tggggtaatt cctcgtataa cgggaactgc ttcatgaaga
     4921 tattcacttc actgcgcaga gcgttcagca cgtcatcgtt ttcggcgttt gtcagcgccc
     4981 ggtcgatcat ctccacgacg gtgatcatat cgacctctct cataccgcgg gtagtaatgg
     5041 ccggaactcc gacccggata ccggaggtga tgaaagcgct tttatcgtca tagggtacca
     5101 tgttcttatt gaccgtgatc tcggctttgc cgagtgtatt ttccgccttt tttccgctga
     5161 tgttcttgtt tcggagatcg atcaggagca gatggttgtc ggttccgccg ctgacgatgt
     5221 gatagccttt ggcgaggaag gctttggcca tggcctgtgc attggaaatg atctgcttgg
     5281 catagaccgt gaaatcgttg gagaggatct ctccaaaggc gacggccttc gcagcgataa
     5341 catgctccag cggaccgccc tgggtgccgg ggaaaacggc catatcgatg agattgctca
     5401 tcggccggat attccctttt acgtctttca gcccgaacgg gttgtcgaaa tcttttttca
     5461 tcaggatgat gccgccccgg ggaccgcgca gggttttatg ggtggtggac gttacgaaat
     5521 ggcaatgttc gaagggacta ttcaggaggc ccttggcgat aagcccggcc ggatgcgcca
     5581 tatcgcacat cacaaatgca ccgacggagt cggctattga acggatccgt gcatagtccc
     5641 agtcgcggct gtaggcgctg gcgccgcaga tgatgagttt ggggcgttct tttttggcca
     5701 cgacctccat catattgtaa tcgaccaggc cgtcctcgcg gccgacacca tatgatacgg
     5761 ggcgatacag cttgccggag aaattgacgg gcgaaccatg cgtcaggtga cctcccatgc
     5821 tgagatcgag acctaaaatg gtatcgccgg gctgaaggat ggcgagcatc agtgcggcat
     5881 tggcctgcgc tccgctgtgc ggctgtacat tggcgtaatc acaactgaat atctgtttga
     5941 gccggtcgat cgcaagctgc tcaacctggt cgacgatctc gcaaccacca taatatcttc
     6001 tgccgggata tccttccgca tatttattgg tcagcaccgt gcccattgct tgcatcactg
     6061 cctgggaagt gaaattttca gatgcgatca actcgatgcc atgacgttgg cgttccagtt
     6121 ctttttcgat gaggtcgaaa actaacgtat ctttttgcat tccgcaaaat taggtcaaaa
     6181 agagtatttt ttcaggtcga gtccgccgaa attctttatt ttacacgcgg agagacaaca
     6241 aattcacatg aaagcgatca ttcccgtagc aggcgcaggt accaaattac gtccgcatac
     6301 gtatacgcaa cccaaggcgt tgatcccttt ggcgggtagg actatcctct cgatcatcgt
     6361 agaccagttg acggaagcgg gaatcaccga attcatcttt attgtcggct atctgggcga
     6421 gaagatacag gacttcgtga aacagaagta tcctcatctg acggctcatt ttgtttatca
     6481 gaacgagcgg cagggtatcg gtcatgcggt cctgctgaca cgtcacatcg tcggcgatga
     6541 cgagttgttc atcgtccttg gcgacaccat ctgtgaatac gatgtcaagg atgtgctgaa
     6601 ccagcctcat tcgctgctcg gcgtcaagcg tgtggacgat ccccgcgttt ttggcgtcgc
     6661 cgagatggga gatgacggga tcatcgagcg ggtgattgaa aaaccccaga tccccaaatc
     6721 caatatggcg ctggtgggta tctatcgcat ccgcgagagc ggcgtgcttt tcaaatgcct
     6781 ggaaaccaat attcagaacc atgtcaccag ctatggcgaa tacagtctca ccgacgccat
     6841 cgagtgtatg atccgcaatg acgtcaaatt ccgggctttc aaggtccaaa actggttcga
     6901 ctgtggtaaa aaggagaccc tgctggaatc caatgccaag ctcttgaaga agtttggcgg
     6961 caccatcgcc ccggagcacc agttcgagaa taccatcatc attcagccgg tgagtatcgc
     7021 ccccggctgc gatatccgga attcgatcgt cggccccaat gtggccatcg gtgagaagac
     7081 ccgggtgaat tattcgatcc tcaaggactc gatcatcggc tctttcgccg acctctatga
     7141 tatcgtgctg acgcattcgc tcatcggcag cgacaccgag gtcaaagggg agtcccggag
     7201 cctgaatatc ggggacaata ctgagatcga tctgggagag tcctagggga acgcccaccg
     7261 cttctaatcc gcggtcccac ctacccattt accccagccg ccagatctgc cggatcctcg
     7321 cgcactccca ccgacggccg atccacccca ttcgctcccc cacaccccga tcacgatttg
     7381 tcggattccg aaaatctgat taaattagtg gtcgaaccac gacaaggaag acaccgctaa
     7441 gctagcccgg acacccgcta acgtttaaaa ctgttttatg ggcacgccaa aaaaattact
     7501 ctttgtcctg ctgttgcccc tggcacttta tgcgaaggcc gggacgggca aactggaagg
     7561 cgttttgaat ggatatgtga cggatgcggt aaccaagaag ccgctttcgg gtgtcgtcgt
     7621 ttccgctttt attcccggta caagtttgtt gaaagaagtt cttaccgacg cggatggata
     7681 cttccggttt gccgagctgc cctcgtcgca ggtgacggtc gagttcaata agaagggata
     7741 tcaacttgcc agacgtccga atgtggccat aaaggaaaaa acatcggtta agctgaatgt
     7801 agagttcctg cccgacgacc ctgactccgg cttgggggga ggagagtacc cactgctgcg
     7861 catgctcgaa gcgaattgat tatacctact cctgataacg taaaacgggg ctgagaacag
     7921 tcccgttttt ttctactaca catgaggctt aaaatctatc ttgaagttac gcctgcctgg
     7981 attgatgcga tgctgcacga ggcagcgctg gtcttccgac cgcctttggc cgggctctac
     8041 agaatcttac gacatctttc gtataaaagt acgccggccg ctcggtaatg ttgcaccgaa
     8101 gctccttgtt tttttgccat tcatccaaag ggccggcgcg cctcaccgat aggacgcaca
     8161 agacatcgtc gacaggacgc acaaggtatc accgatagga cgcacgtcat cccgtaaagg
     8221 ttactgaaag gtgccgcggc aggcgactat ttgtggaatg cccatttcca catgatcttc
     8281 caggtcggct tcttaccata cattagtata cctgtccggt agatttttcc ggccagccac
     8341 accgtaaaga gaaaacccag tataaggaac agcatcgaca gccctagctg ccaggccggg
     8401 accccgaacg ggatacgcgc catcatgacg atgggactgc tgaatgggat gatgctcgcc
     8461 catacggcca gcgccccgct ggtatcctgg acggccacgg acatgatgat aaaggagaag
     8521 atgacgggca tggtgatcgg caacatcagc gattgggctt cctgcggatc ttcattgacg
     8581 gcgctgccca cggcggcaaa cagtgaagaa tagaacaggt atccaccaat gaaatagaac
     8641 aggaaacaac ccaggatcag ccacaggttg gccgtactca gcgcgccaag taccttggga
     8701 agcagctgtg ccttctcgct ggcctgggca gcggaggcat tccccaccgc acccgcctgt
     8761 tgggcctgtt gcacctgctg ccaggtgtca tgcgagacga aaccctgggc tacggccgcg
     8821 agtccgccga tgagtatcac ccacagcagg aattgcgtaa gaccaacggc cccgataccc
     8881 gtgatctttc ccatcatcag ttggaagggt cgcaccgagc tgacgacgac ttcggcgata
     8941 cggttggtct tttcttccat gaccccgcgc atgaccatgg cgccgtagat aaaggtgagc
     9001 agatagatga gaaaaccgct gacatacccg atcacatagg acgaggtctg gttggaggcc
     9061 ttggcttcct tgccca
//
LOCUS       DASYSV010000556        13783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_238316, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000556 DASYSV010000000
VERSION     DASYSV010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13783
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238316"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VGQ51_17340"
     CDS             <1..558
                     /locus_tag="VGQ51_17340"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEV9038406.1"
                     /translation="TRIMIGWDCFSLTLIIFSVIIFTSMRPREIRVLAKQEDAGRIVV
                     FFIVLVATFGSLMGVLLLLQNREPWLLGKGMETFIYLTGVVCSWMLLHTMFAYRYAFL
                     YYGDHPLDPDPRTAALQIPNELWPDYLDFCYFSFVIGMTFQVSDIEISSRLIRRVALL
                     HGMLSFLFNTVIVALTINVIVDLKG"
     gene            complement(722..1375)
                     /locus_tag="VGQ51_17345"
     CDS             complement(722..1375)
                     /locus_tag="VGQ51_17345"
                     /inference="COORDINATES: protein motif:HMM:NF016283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEV9038407.1"
                     /translation="MRRLHNPDFNYGEYAIIRSQAGLNTYKISVKEWIEKTKAIGLKA
                     SAGRYGGTYAHKDIAFEFGMWVSAEFKIYLIKEFQRLKDEENSVRNFGWNIQRILSKV
                     NYRIHTDAIKEALIPPTITREQSIVVYANEADLLNMAIFGQTAAIWRQKNADKSGNIR
                     DEATLEQLVVLTNLESINALLIRQGLPQSQRLQKLNETATQQMRSLLSNPTVKKLDC"
     gene            complement(1421..2032)
                     /locus_tag="VGQ51_17350"
     CDS             complement(1421..2032)
                     /locus_tag="VGQ51_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038408.1"
                     /translation="MNIATPYRLPVAAAFFLVVFASSCTTLNIKNYYHRHRPTLDSIE
                     ETFRHAWQAKPFSIEFTDRSFDRVSIELITDTLTYIYEYDVGETRLHDTLVKYGYDPV
                     PVRWLIGRMRRMNCTWIDRLDYYGDTSRHSLIYIALWPRTFNRPFVDKKYYILTYFQQ
                     PQYFDSEGRLLTGRRMHHIRKINAEVYRRINDKVCYTISDRFR"
     gene            complement(2029..2979)
                     /locus_tag="VGQ51_17355"
     CDS             complement(2029..2979)
                     /locus_tag="VGQ51_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038409.1"
                     /translation="MPRTALLTLLIFLVIRGASAQPAAISPPATQPTSTPQQALALLE
                     HTTLPDSSKWWPNIRRVLFIENLRANILYPLKIYQGSNTNFCGYAALSYLPLHNDPLT
                     YTRFMLDLYIDGHASYGKIRFDPSPEIHHAAGTLRFKGILDIRPADQLWFLALADHFR
                     TYLNFFHRHFHTGSEDTFWAAVSYGKFNRMIHELLGYDVHAKGSDLFRPHIPDLYGCL
                     KDDLARPGTTFLYVNNTYLHKKNHDRYKSNFPTHFIVLDDIHRIDVPPSAVGSHVPED
                     DMVDIVYWDYGGRTLRQVSLRFLKKIVFGVTHCSPPSQNP"
     gene            3008..5608
                     /locus_tag="VGQ51_17360"
     CDS             3008..5608
                     /locus_tag="VGQ51_17360"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV9038410.1"
                     /translation="MKGIFLAGSMMAVSLGLCAQRNGDSLREGGRRVENVLWYVQPAK
                     VWTEALPLGNGKMGAMVFGGIGDERIALNDATLWSGGPKRGPVNPGARQWLAPLRKAL
                     FAEDYPEADSLARKMQGVWSESYLPLGDLHIHQDFGARGRQGSGGGAAGGDTTLGEVG
                     AGSRAAGSASEDDPVISRYRRELDIQDAVARTRYTVNGVEYTREAFVSAPDGVMVVRW
                     TCDKPGALNLRISASSLLQHRFQYRFGEGWGIGGKAPVHVEPNYVNSKVPVLFDDEDT
                     CAGMGWVLRAGVIHRGGEVIKYIGEIRATGVNELVLYVAVSTGFRGFDRCPFGVGDAQ
                     AVLQKAMKKSYAALWEAHEKDVHGYFNRVSLRLGHAGKDAELPTDQRLAAYTKGEHDP
                     GLEALYFQFGRYLLMSSSRTPGVPANLQGIWNAELQAPWSSNYTTNINVEMNYWPAEE
                     CNLSEMTRPLFGLIKGLSVNGARAAKEYYGASGWVTHHNSDEWAMATPVGGMGHGDPK
                     WANWPMGGAWLVRHLWEHYLYTGNERFLRDTAYPLMKGATRFILDWLVEAGPAPKSEG
                     RQGSAPLLVTAPSFSPENNFYYSDKKIAEVSVATTMDMGIIRELFDNMLAAEKVLGVD
                     RDLKIAADRKRLFPYQVGGEGQLQEWYKDYQSPEPHHRHVSHLYSVYPASLISVDSTP
                     ELAAAAKRSLELRGDGGTGWSMAWKVSLWARLRDGNHAHRLFTNLLQLTHEDAHTGAG
                     GVYPDLFDACPPFQIDGNFGGTAGIAEMLLQSQEGIVDLLPALPDEWPMGEFRGLVAR
                     GGFVVDASWKDGKIVEAVVMSRADRVCKVRSAVPLRMDGATSKKSGNGWIVEWNAKKG
                     VKYRLLNAPA"
     gene            6018..7055
                     /locus_tag="VGQ51_17365"
     CDS             6018..7055
                     /locus_tag="VGQ51_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038411.1"
                     /translation="MKKLCLLLIGGFGVLTVMAQSSDRQPFMTKTFSRAGVKAIEVET
                     SGGNISVEGTASGDARVEVYISGDGRHEASKEEIQKMLDQQYDLEVAMEGNKLVAVAK
                     QKSGWSNMWRRGLSISFRVFSPEAVGSHVRTSGGNISMRNLSGAQDFRTSGGNLDVEH
                     LTGAIIGRTSGGNVSISDSRDDIDLETSGGSMNATRCEGKIDLETSGGNVSLKSVKGT
                     IHARTSGGTVEGGKIDGELQAHTSGGNIDLDDVAASLQASTSGGSIHVEFLSTGKYVN
                     LSNSGGDISLQLPQGQGVDLRISADRIHTSSMGNFRGDLDEHHMNGTLNGGGTPVKVD
                     GGGGSVHLSFK"
     gene            complement(7101..8792)
                     /locus_tag="VGQ51_17370"
     CDS             complement(7101..8792)
                     /locus_tag="VGQ51_17370"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9038412.1"
                     /translation="MNKQLYNLRFLLPALASILLLGACKKSILQLPSPNAPTPQSSLI
                     TEGGIDAFAQGIYYKWIAFETGDGNLNFFDIAWYIESNMGDEDFTPYSNFGSRYPMNI
                     ATITLPAPYGTVIRNPSGFPTQLDILRSFNTRAAGDGNSIQYEWDCFYYINAQANTLI
                     QALTNPALKLSGDAATKIKLLQAWAYYWKGYSYSKIGSMYLAGVIDDSPDSTTKGFTS
                     NMYVPHDQVIAAANKNFDQAASIFGAITENADYDATFKLMVPTFNLPNNVITPAMWVR
                     QIKSFEARNYMANRKVKTMTSADWTAIQTLAAAGMTPNDNTLKWGVAPAGIPDLTKGL
                     GFLFHPMMLHSYSGGLSFVSERLMQDIMPGDQRLSAFKPYPGGAVVNVLNRGIQFGTR
                     YIPIDIEDAGGKGKYATENYSPLAQVPIAPTWEETALMTAEADIHLGSVDAGLQLIDA
                     VRASQNAGLPKLAGSGISADSALVHLRSERRIGLYLHNVSWYDARRWGINEPVAQGGG
                     RIGNIVVPGSLIGPSGTPATVLPCQIDYSFSDYWDVPQNELDFNPATTGSAPIKN"
     gene            complement(8818..11973)
                     /locus_tag="VGQ51_17375"
     CDS             complement(8818..11973)
                     /locus_tag="VGQ51_17375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9038413.1"
                     /translation="MRVLLLVAVAVLFACIEGQAQQTITVSGKVTDEKGAAIAGATIT
                     QKGTRNATTSHDDGSWTLQVPNKAKLIISYVGYEPREIESRTGLTISLAPDVKSLSDV
                     VVTGVGVATNRKKVPIDVGTVSSKDFAPSVTTNVQQALDGQIAGASVQQSSGTPGAGF
                     NIILRAVNSLDNTNPLILVDGVQMNQANNINNIDPSIVDHIEVVKGPAGAMLYGAQGA
                     NGVIQVFTKKGTLNGRMTINFNSKVSVDNILTGRHPLLSTHHHYVTDANNNMLDSKGN
                     PIAKDATGIWSDPQVPIATAPANAFLTNDKTYNIPIYDHIKQGFRQALTFSNSINISG
                     GTQNSDYAITGSQLNQQDVLSNAYSRTNLSVNLGIHPFKGFTFRTITQGISNYQNLLN
                     GNRFNMLTNYNFVDFNWKDSTGHYPLKTVNSSGGYNTLSENQYHHQNHSILEIFQNFD
                     FNYKFPRYVELDVKYGLDYNADDFSNYYQNQTANLQYVKFNTYWGPSGSGAITNTMSK
                     NFYQNGLYSAFFRTDFEKDFNSHLPITTTTEFAYDYRKSQFRQYYAEGIGLPTYPPPN
                     INQATTKTAGDNYSEQITYGLLVNQTIDWGNLFGVSGGFRSDYGSAFGAAQNAAVLAR
                     GTFYFRPSELMPTQQSWLKDWKIRTAYGAAGVQPGPYDRQSLLTPATLGTGIAIALPS
                     QANNANLRLATNYELEIGTDFTVTPFEGSWLSRFVFAGTYWNRKTNDTYQNAQVAPST
                     GYATKLDNLTTITSHGFDISLDASIYNGRNITWDVSTRWGLSRAVVNKIANGQDVVDF
                     AFALKQGKPLGLFYVQTPLHSVGQVGPDGKTPLIPAASQGNYSVTSTGMVVLNSDNYV
                     QYTPTNNLSAVGHAYPDFNSSLINRFTFFKTLSVSFQFDWVYGNSIYNVTKQWLYRPT
                     GGTGGQGGESSDLDKKITIQGQTGSFVNYYNSIYNVGLPVSPFIENGSYIRLRDLSIG
                     YDLTHYVIGSRVIKRLTVTASGRNLLTFTKYSGMDPENIGAYDEGGNNLSGQRIGAFT
                     GVDYYSTPNLRSYQFALNVGF"
     gene            12117..13466
                     /locus_tag="VGQ51_17380"
     CDS             12117..13466
                     /locus_tag="VGQ51_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV9038414.1"
                     /translation="MKKTTLMLLLAGVVITCPAQTTIVRDPEIAAMVKEVNADSLQSY
                     IKSMVAFGTRSTVSSVTDKKRGIGAARNWVLMKFEQFAAASGGRLTAFVDTTTYAADG
                     RRVKEPINLGNTVATLKGTDPSYNRIFIISGHLDSRRTDVMDGTNDAPGANDDGSGSA
                     AVLECARVMSKHHFPATIVFVTVSGEEQGLLGSGYMAKKAKAAGWNIEAVLNNDIIGS
                     NNSNETNIIDNTRVRVFSEGLAFADTGRMVSRIRSMGLENDGKSRQLARYVKEIGERY
                     VDNLQVVMVYRPDRFLRGGDQIPYQQNGYTAVRITEMNENFNHQHQDVRTDKGVVYGD
                     LAEFMDFEYLRKNTAMNLANLGNLAKSPGMPQDVRIEVRGLGNGTSLSWKAPVDGKPK
                     GYFVLIRETTSAVWEKKMFFAGTEANLPYSKDNYFFAVQSVSEEGNESLPVVPVPGR"
     gene            13514..>13783
                     /locus_tag="VGQ51_17385"
     CDS             13514..>13783
                     /locus_tag="VGQ51_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038415.1"
                     /translation="MIRNYFKTAIRNLWKSKGFSAINIIGLAIGLATCLLILIFVMDE
                     LGYDRYNEKADRIYRVDGDIKFGGNHFILAVAPAPAGPALLRDYPE"
BASE COUNT         2974 a         2894 c         4606 g         3309 t
ORIGIN      
        1 accaggatca tgataggatg ggattgtttc agtcttactt tgatcatttt cagtgttatc
       61 atttttacgt ctatgcggcc ccgggagata cgggtcctgg ccaaacagga agatgccggg
      121 aggatcgtgg tattctttat cgttttggtc gcgacgtttg gcagccttat gggggtattg
      181 ctgctgttgc agaacaggga accctggttg ttggggaaag gaatggagac gttcatttat
      241 ctcaccgggg ttgtctgctc gtggatgttg ctgcatacga tgttcgccta tcgttatgct
      301 tttttgtatt atggcgatca tcctttggat cccgatccgc ggacggccgc tttacagata
      361 ccgaatgagc tgtggccgga ttatctggat ttttgttatt tttcgtttgt gatagggatg
      421 acgttccagg tgtcggatat cgagatctcg tcgagattga tacggagggt tgcgttgctg
      481 catgggatgc tgtcgttcct gttcaatacg gtgattgtcg cgttgacgat caatgtgatt
      541 gtagatctga aagggtagtg agagagagac gcttatgatg ggccttcggc cggccggccc
      601 gtggtcggcc ctggtgattg tggatctgaa agggtagtga gagagagacg cttatgatgg
      661 gccttcggcc ggccgacccg tggtcggccg tggtgattgt ggatctgaag gggtgaggac
      721 gtcaacaatc caattttttg acagtgggat tagataacaa agagcgcatt tgttgggtag
      781 ctgtttcatt aagtttttga agacgttggc tttgcgggag cccttgcctg atcaacaatg
      841 cattgatact ttcgagattc gtaagtacaa ctagttgttc aagggtcgct tcatccctga
      901 tgttcccact tttatctgca tttttttggc gccagatcgc ggcagtttgt ccgaatatcg
      961 ccatgttcaa cagatcggct tcattagcat aaacgacaat ggattgttcg cgggtgatgg
     1021 taggagggat caatgcctct ttaatggcat ccgtgtgaat tctataattg acctttgaca
     1081 aaatacgctg tatgttccaa ccaaaattcc tgacgctatt ctcttcatct tttaaacgtt
     1141 ggaattcttt tataaggtaa atcttgaatt cagcactgac ccacatcccg aattcaaaag
     1201 cgatatcctt gtgagcgtaa gtgcctccat atcttcctgc ggaggccttt aaacctattg
     1261 ctttggtctt ttctatccac tctttgacgc ttattttata ggtgttcaga cctgcctggg
     1321 atctaattat ggcatattcg ccgtaattaa aatctggatt atgaaggcgt cgaatttact
     1381 aattttttta gcgatgcgga taattttatt ggatgcggcg tcagcggaaa cggtcggaga
     1441 tggtgtagca caccttgtcg ttgatgcggc ggtagacttc ggcgttgatc ttgcggatat
     1501 ggtgcatgcg gcggccggtg aggaggcggc cttcgctgtc gaaatactgc ggctgctgga
     1561 agtaggtgag gatataatat tttttatcga cgaagggtcg gttgaatgtt cgcggccata
     1621 gagcgatata gatcagcgaa tgccgggagg tgtcgccata atagtccaat ctgtcgatcc
     1681 aggtgcaatt catccttcgc atgcgcccga tgagccagcg gaccgggacg gggtcatagc
     1741 catattttac gagggtgtcg tgaaggcggg tctcgccgac atcgtattca tagatataag
     1801 taagagtatc ggtgatcagc tcgatagaga cgcggtcgaa ggaccggtcg gtaaactcga
     1861 tagagaaggg ttttgcctgc caggcatgac ggaaggtttc ttcgatgctg tcgagcgtgg
     1921 gacggtggcg gtggtaataa tttttgatat tgagggtcgt gcaggaggag gcgaagacca
     1981 ccaggaagaa ggcagcggct accgggagac gatatggggt ggcaatgttc atgggttctg
     2041 tgacggcggt gagcaatggg tgacgccgaa aacgatcttt ttcaggaagc ggaggctcac
     2101 ctggcgaagt gtccttccac cgtagtccca ataaacgata tcgaccatgt catcttcggg
     2161 gacatgcgag ccgactgcag acggcgggac gtcgatgcga tggatgtcgt caaggacgat
     2221 gaaatgggtc gggaaattgc ttttgtatct atcatggttc tttttgtgga gataggtatt
     2281 attgacataa aggaaggtgg tgccaggacg ggcgagatcg tccttgagac agccgtatag
     2341 atcggggata tggggtcgga aaagatcgga gcctttggca tgaacgtcgt agccgagcag
     2401 ctcgtggatc atacggttga acttgccata gctgacggct gcccagaagg tgtcttcgga
     2461 accggtgtga aaatgccggt ggaagaaatt gaggtaggtg cggaaatggt cggccagcgc
     2521 gaggaaccaa agctggtcgg cgggccggat gtcgaggata cccttgaaac ggagcgtgcc
     2581 ggctgcatga tggatctccg gggatgggtc gaaacggatc tttccatagc tggcgtgacc
     2641 atcgatatag aggtccagca tgaagcgcgt gtaggtcagg gggtcgttgt gaagggggag
     2701 gtatgacagc gccgcatagc cgcagaaatt ggtattgctg ccctgataga tcttcagagg
     2761 atagaggata ttggcgcgaa ggttttcgat gaacagtacg cgccggatat tgggccacca
     2821 tttgctggaa tcgggaaggg tggtatgctc gaggagtgcg agcgcctgtt ggggggtgga
     2881 agtgggttgg gtagcgggag gtgagattgc ggcgggttgg gcggaagcac cccggatcac
     2941 gaggaagatc agcaacgtca atagggctgt gcggggcatg atcttaaatt taatgtatct
     3001 tgatggtatg aaaggtattt ttttggcagg cagcatgatg gccgttagtt tggggctttg
     3061 cgcgcagcgg aatggggata gcttacggga aggcgggcgc cgcgtcgaaa acgtattgtg
     3121 gtatgtgcaa ccggcgaaag tttggacgga ggcgttgccg ttgggtaacg ggaagatggg
     3181 agcgatggtg ttcggcggta tcggcgacga gaggatcgcg ctgaatgacg ccacgttgtg
     3241 gtcgggcgga cctaagcggg ggcccgtgaa tccgggggcg aggcaatggc tggcgccatt
     3301 gcggaaggcg ttgtttgcgg aggattatcc ggaggcggat tcattggcga ggaagatgca
     3361 gggggtatgg tcggagagtt atctgccgtt gggggatctg catattcacc aggattttgg
     3421 agcgcggggg cggcagggct cgggaggcgg ggcggctggc ggagatacga cattggggga
     3481 ggtgggtgca ggaagcaggg cggctggcag tgcgtcggaa gacgatccgg tcatcagcag
     3541 gtatcggcgc gagctggata tacaggacgc ggtggcgagg acaaggtata cggttaacgg
     3601 ggtagaatat acgcgcgagg cgtttgtttc ggcgccggat ggggtgatgg tggtaaggtg
     3661 gacgtgtgat aagccgggag cgttgaattt acggatatct gcgtcgagtt tgttgcagca
     3721 tcgttttcag tatcggttcg gtgaggggtg ggggatcggc ggcaaggcgc cggtgcatgt
     3781 cgagccgaac tatgtgaaca gcaaggtccc ggtgctcttt gacgacgagg acacgtgtgc
     3841 cgggatgggg tgggtgttgc gggccggggt gatacatcgg ggcggagagg tgattaagta
     3901 tataggagag atacgtgcga ccggtgtaaa tgaactggtg ctttatgttg ctgtttcgac
     3961 gggattcagg ggattcgatc gttgtccttt cggtgtgggg gatgcgcagg cggtgctgca
     4021 aaaggcgatg aaaaagtctt acgccgcgtt gtgggaggcg catgagaagg atgtacatgg
     4081 atatttcaac cgtgtttcgt tgcggttggg gcatgcgggc aaggacgcag aattgccgac
     4141 cgatcagcgg ctcgctgctt atacgaaggg agagcacgat ccgggtctgg aggccttgta
     4201 ttttcagttt gggcgatatc ttttgatgag cagttcgcgg acgccggggg tgccggcgaa
     4261 cctgcagggt atctggaatg cggagctgca ggcgccctgg tcttcgaact acacgaccaa
     4321 tatcaacgtg gagatgaatt actggccggc agaggaatgc aacctgagtg agatgacgcg
     4381 gccgttgttc ggattgataa aaggtctgtc ggtgaatggg gcgcgggcgg cgaaggaata
     4441 ttatggggca tcgggatggg tgacgcatca taacagcgat gaatgggcga tggcgacgcc
     4501 tgtgggaggg atgggacatg gggacccgaa gtgggccaac tggccgatgg gcggggcctg
     4561 gctggtgcgg catctttggg agcattattt gtatacggga aacgagcgat ttttaaggga
     4621 tacggcgtat cccttgatga agggggcgac gcggttcata ctggattggt tggtggaagc
     4681 aggaccggcg cccaagagtg aggggcgcca ggggtccgcc ccgctgctgg tgacggcgcc
     4741 gtctttttcg cctgagaata atttttatta ttccgataag aaaattgcgg aggtatcggt
     4801 ggcgacgacg atggacatgg ggatcatccg ggagctgttc gataatatgc tggcggcgga
     4861 gaaggtgctt ggcgtggata gggacctgaa aattgcggcg gacaggaaac ggttatttcc
     4921 ttaccaggtg ggcggcgaag ggcagttgca ggagtggtat aaggattacc agtcgcccga
     4981 gccgcatcac cggcatgtat cgcatttgta ctcggtgtat ccggccagcc tgatatcggt
     5041 ggatagtacg cccgaattgg cggcggcagc gaaaagatcg ttggagttga gaggtgatgg
     5101 cggtacgggc tggagcatgg cctggaaagt gagtctatgg gcgcggttgc gggatgggaa
     5161 tcatgctcac cggttgttca ccaatctgct tcagctgacg catgaagatg cccataccgg
     5221 tgccggcggc gtatatccgg atcttttcga tgcgtgtccg ccctttcaaa tagacgggaa
     5281 tttcggcgga acggcgggta ttgcggagat gttattgcaa agccaggagg ggatagtcga
     5341 tctgttgccg gcgttaccgg atgagtggcc tatgggcgag ttcagaggcc tggtggcccg
     5401 tggcgggttc gtggtggacg cttcgtggaa ggatgggaag atagtggagg ctgtggtcat
     5461 gtcgcgggcg gatagggttt gcaaggtgcg gagtgcggtt ccactgcgga tggatggcgc
     5521 gacgtcgaag aaatccggaa acgggtggat cgtagaatgg aatgcgaaga agggggtgaa
     5581 gtatcggcta ttgaatgcac cagcatagtt cagtttccag ccgggatgta tcttccgtcc
     5641 aatggccata gatctccaga cagggcagac aatggtttat ccctctccgg tccagatttg
     5701 agacccggga atacttctat tattttgtca tcaggtccga tatgtttgag gtccggggca
     5761 ctttttttag cataagggta acatgtttgg cggtgggatc gtcgtagatg ggcggggcag
     5821 tggggaatgg ggagtgcggc ggggccgtcg tagatgggca gggcagtggg gaatgaggag
     5881 tgcggcgggg ccgtcgtaga tggaagcgcc agccgggaaa gcgggagggc ggcgatccgc
     5941 ggcagtgggc ggcaggagga gggacgtgca acaatcgggc ggcgggagcc gacattagga
     6001 aataaaaact aacacatatg aaaaaacttt gtttgttatt gatcgggggt ttcggggtgc
     6061 tgacggttat ggcgcagtct tctgatcgtc agccctttat gaccaagact ttttcgcgcg
     6121 cgggggttaa ggcgatagag gtggagacat ccggagggaa tatttcggtg gaagggacgg
     6181 cctcggggga tgcccgggtg gaagtctata tttcgggtga cgggcggcat gaagccagca
     6241 aggaagagat ccagaaaatg ctggatcagc aatatgatct cgaggtagcg atggaaggca
     6301 ataagctggt tgcggtggcg aagcagaagt cgggctggtc gaatatgtgg cggagggggt
     6361 tatcgatatc tttccgcgtc tttagcccgg aggctgtggg gagccatgtg cggaccagtg
     6421 ggggcaatat ttcgatgcgg aatttgtcgg gggcgcagga tttccggacc agtggaggca
     6481 acctggatgt cgagcatctg acgggcgcca tcatcggaag gaccagcggt ggcaacgtgt
     6541 cgatcagcga cagccgggac gatatcgatc tggagaccag cggcggcagt atgaatgcca
     6601 cgcgttgtga aggaaagatc gatctggaga cctcgggagg aaatgtttcg ttgaaaagcg
     6661 tcaagggcac catccatgcc cggacttcgg gcggcaccgt cgaggggggc aagatcgatg
     6721 gcgaattaca ggcgcatacg tcgggtggga atatcgatct ggatgatgta gcggcaagcc
     6781 ttcaggcgtc gacgagcggg ggcagtatcc atgtggaatt tctgtcgacc ggcaaatatg
     6841 tgaatctgtc caattccggc ggggatatca gcctgcagct gccgcagggg cagggggtcg
     6901 atctaaggat aagcgccgac cggatccata cttcgtcgat gggcaatttc cggggggatc
     6961 tggatgagca tcatatgaat gggacgttga atggaggagg gacaccggtg aaggtggatg
     7021 gaggcggagg gtcggtacat cttagcttta aataaaaaaa acccggcgag aagccgggtt
     7081 ttttgtgtgc cggggtgtcg ttagttcttg atgggggcag aacccgtcgt ggccgggttg
     7141 aagtccagct cgttttgcgg tacgtcccag taatcggaaa aggaatagtc gatctggcag
     7201 ggaagtaccg tggcaggggt gccggagggg ccgatgaggc tgccgggtac tacgatattc
     7261 ccgatccttc cgccgccctg tgcgacgggc tcgttgatgc cccagcggcg ggcatcgtac
     7321 caggatacgt tgtggagata gaggcctatg cggcgctcgc tacgaaggtg tacgagtgcg
     7381 gagtccgcgg atatgccgga acctgccagt ttgggcaagc ccgcattttg cgaggcccgg
     7441 acggcgtcga tcagctgaag gcctgcgtct acacttccca gatggatatc ggcctctgcg
     7501 gtcatgaggg cggtctcttc ccaggtgggg gcaataggga cctgggccag cggagaataa
     7561 ttctccgtcg catatttgcc cttgccgccg gcatcttcta tatcgatggg aatgtagcgt
     7621 gtgccaaact ggatgccacg gttaagcaca ttgaccacgg cgccgccagg atagggttta
     7681 aaggcgctga ggcgctggtc tccgggcatg atatcctgca taagccgttc gcttacgaac
     7741 gacaatccac cgctgtacga atgcagcatc atgggatgga acaggaatcc gaggccctta
     7801 gtcaggtcgg gaatcccggc tggagccaca ccccatttga gcgtattgtc attcggcgtc
     7861 atgcctgcgg ctgccagggt ttgtatagcc gtccagtcgg cggatgtcat ggtcttgacc
     7921 ttgcggttgg ccatatagtt gcgggcctcg aagcttttga tctggcggac ccacatggcg
     7981 ggcgtgataa cattattggg caggttgaac gtgggtacca tgagcttgaa cgtcgcatca
     8041 taatccgcat tttcggtaat ggccccgaag atcgaagctg cctgatcgaa attcttatta
     8101 gcagcggcga tgacctggtc gtgcgggaca tacatattgc tggtgaaacc tttggtcgta
     8161 ctatccgggc tgtcatcgat gacgccggcc aggtacatcg atccgatctt ggagtaggag
     8221 tagcctttcc agtagtaggc ccaggcctgg agaagtttga tcttggtcgc ggcgtcaccg
     8281 ctgagtttga gcgcgggatt ggtcaatgcc tgaatgagcg tattggcctg tgcattgata
     8341 tagtaaaagc agtcccattc gtattggatg gaattgccat cgccggcggc ccgggtattg
     8401 aaggagcgga gaatgtccag ctgtgtggga aagccggaag ggttcctgat gacggtaccg
     8461 tagggcgccg gtagggtaat ggtggcgata ttcatcggat agcgggaacc gaagttcgag
     8521 taaggggtga agtcttcgtc gcccatattg ctttcgatat accaggcgat atcgaagaaa
     8581 ttgaggttgc cgtcacccgt ctcgaaggcg atccatttgt agtaaatgcc ctgggcaaag
     8641 gcatcaatgc cgccttcggt gatcagggag ctctgtgggg tcggcgcatt ggggctgggc
     8701 agctgcagga tcgatttctt acaggctccc agcagcagga tcgatgccag cgcaggcagc
     8761 aagaaacgta ggttatatag ttgcttattc atatagtttc atgatttgag tgatcgatta
     8821 gaatccgaca ttcagggcaa attgatagga ccttagattg ggagtgctat aatagtcgac
     8881 gccggtgaag gctccgatcc gttgtcccga gaggttgttg cccccttcgt catatgcgcc
     8941 gatgttttcc ggatccatgc cgctgtattt cgtaaaggtg agcaggttgc ggccggacgc
     9001 cgtaacggtc agccgtttga tgaccctgct gccgatgacg taatgggtca gatcataccc
     9061 gatggacaga tcgcggaggc gtatatagga accgttctcg atgaaaggag agacgggcag
     9121 acctacgttg tagatagagt tgtaatagtt gacgaatgaa ccggtctgac cctggatggt
     9181 gatctttttg tccaggtcgc tggattcgcc gccctgtcca cccgttccgc ccgtgggtct
     9241 gtacaaccat tgtttggtga cgttgtagat gctattacca tagacccaat cgaactggaa
     9301 ggagacgcta agcgttttga agaacgtgaa gcggttgatc agtgaggagt tgaagtccgg
     9361 ataggcgtgt cccacggccg acagattgtt cgttggcgtg tactgcacgt agttgtcgct
     9421 gttgagcacc accattcccg tacttgtgac cgaataattc ccctgactgg ctgcggggat
     9481 gaggggtgtc tttccatccg gtcccacctg gcccaccgag tgcagcggag tttgaacgta
     9541 gaacagaccc agtggtttgc cttgtttgag tgcgaaggcg aagtcgacga catcctggcc
     9601 attggcgatc ttgttcacga cagcacggct gagtccccag cgtgtgctga cgtcccaggt
     9661 gatgttacga ccgttgtata tactggcgtc cagggagatg tcgaagccgt gagaggtgat
     9721 agtggtgaga ttgtcgagct tggtggcgta gccggtggaa ggagctacct gcgcattctg
     9781 ataggtatca ttggtcttgc gattccaata ggtgcctgcg aatacaaatc gcgacagcca
     9841 tgagccctca aaaggggtga cggtgaaatc ggtaccgatc tccagctcat agttggtcgc
     9901 cagtctgagg ttggcgttat tagcctgtga tggaagggca atggcgatcc cggtgcccag
     9961 cgtggccggt gtgagcagtg actggcgatc gtatgggccg ggttgaacgc ctgctgcacc
    10021 gtatgccgta cggatcttcc agtccttgag ccaggattgc tgggtcggca tgagctcgga
    10081 tggacggaaa tagaaagttc cccgggcgag gacggctgca ttttgcgcgg caccgaatgc
    10141 gctgccgtaa tccgaacgga acccgccgga tacgccgaat aggttgcccc agtcgatggt
    10201 ctggttgaca agaagaccat acgtgatctg ctcggagtag ttgtctcccg cggtcttggt
    10261 tgttgcctga ttgatgttcg gcggcgggta ggtgggtagg ccgatgcctt ccgcatagta
    10321 ttgtctgaac tgagatttcc ggtaatcgta ggcgaactcg gtagtcgtcg ttatcggcaa
    10381 atgggaattg aagtctttct cgaaatcggt gcggaagaag gcggagtaca aaccgttctg
    10441 atagaaattc ttgctcatcg tgttcgtaat ggcgcccgat ccgctgggtc cccaataggt
    10501 attgaatttg acgtattgga ggtttgcggt ctggttctga tagtagttgg agaaatcgtc
    10561 ggcgttgtaa tccagtccat atttgacatc gagctctacg tatcggggga acttataatt
    10621 gaaatcgaag ttctgaaaga tctccagtat cgagtggttc tgatggtggt attggttctc
    10681 cgagagggta ttatagccgc cggaagaatt gaccgttttc aatgggtagt ggcccgtgga
    10741 gtctttccag ttgaagtcga cgaaattgta gttcgtcagc atattgaacc tgttgccatt
    10801 gagcaggttc tggtagttgg atatcccctg ggtgatggtc cggaaggtaa atcctttgaa
    10861 aggatggata ccgaggttga cagagagatt cgtgcggcta taggcattgc tgagcacgtc
    10921 ctgctggttc agctgggagc ctgtgatggc atagtccgaa ttctgggtgc cgccgctgat
    10981 atttatcgaa ttcgaaaagg tcaacgcctg acggaatccc tgttttatat gatcgtaaat
    11041 ggggatatta tacgtcttgt cgttggtgag gaaggcattg gccggcgccg tggcgatggg
    11101 cacctgcgga tcgctccata tgcccgtggc atcctttgcg ataggattgc ctttactgtc
    11161 cagcatatta ttgttggcat cggtgacata gtggtgatgg gtgctaagga gcggatgccg
    11221 gccggtcaga atgttgtcga cgctgacctt ggagttgaaa ttgatggtca ttctaccatt
    11281 cagggtacct tttttggtga agacctggat aacgccgttt gcgccctgcg cgccatagag
    11341 catagcgccg gcgggacctt ttacgacttc gatatggtcc acaatggagg ggtcgatgtt
    11401 attgatattg ttggcctggt tcatctgcac cccatcgacg agtatcaggg ggttggtgtt
    11461 gtccaggctg ttgacagccc gaaggatgat attgaaaccg gcgccgggcg taccgctgct
    11521 ttgctggacg ctcgcgcctg cgatctggcc gtccagggcc tgctggacgt tggtagtgac
    11581 gctgggggca aagtcttttg aagaaacggt ccctacatcg atgggaacct ttttccgatt
    11641 ggtcgccacg cccacgcccg taaccacgac atcggaaagc gacttgacgt cgggagccag
    11701 ggagatggtc agaccggttc gggattcgat ctctctgggt tcatagccca catagctgat
    11761 gatcagcttt gccttgttgg gtacctggag ggtccaggag ccatcatcgt gagaagtggt
    11821 ggcattgcgg gtaccttttt gagtgatggt ggcgcctgcg atggcggcgc ctttttcgtc
    11881 ggtaactttt cctgagacag ttatcgtctg ctgggcttgt ccttcaatac aggcaaaaag
    11941 aacagccaca gccacaagca gcagtactct cataattgtt ttggttgagg atgaagtaat
    12001 tggtcctggt caatttaaga tgagtttggc gaaggagtcc ggagccggcc actaagaaaa
    12061 aggtctagca aatactaata attcgcgtag ttgggaaaaa aaattaaaat tgggttatga
    12121 aaaagacaac tctaatgctt ttgctggcag gcgtcgtgat cacctgtcct gcgcaaacga
    12181 cgatcgtccg tgacccggag attgccgcca tggtcaagga agtgaatgcc gacagccttc
    12241 aatcgtatat taaaagtatg gtagcgtttg gtacccgaag cactgtcagt tcagtaaccg
    12301 acaaaaaacg aggtatcggt gcggcgcgga actgggtact gatgaagttc gagcagtttg
    12361 cggccgcctc gggcggccgg cttacggctt ttgtagacac gacgacctat gcggccgatg
    12421 gcaggagggt gaaggagccg atcaatctgg gtaataccgt agcaacgttg aaggggacgg
    12481 atccttccta caaccgcatt tttattatca gcgggcattt agacagccgg cggacggatg
    12541 tcatggatgg tacgaacgat gctccgggcg ccaatgatga cggcagcggg agtgcagcgg
    12601 tcctggagtg tgcccgggtc atgagcaagc atcattttcc cgcgacgatc gttttcgtga
    12661 cggtgagcgg tgaggagcag ggtctgctgg ggtctggtta tatggcaaaa aaggcgaagg
    12721 cggcgggatg gaatatcgag gccgtgctca acaacgatat cattggtagc aataacagca
    12781 atgagaccaa tatcatcgac aatacgcgtg taagggtttt cagtgagggt ctggcgtttg
    12841 cagacaccgg cagaatggtc agcaggatcc gatcgatggg gttggagaat gacggaaagt
    12901 cgcggcagct ggcgcggtat gtgaaggaga tcggggagcg gtatgtggac aatctgcagg
    12961 tggtgatggt gtatcggccg gacaggttcc ttcggggtgg agatcagatc ccctaccagc
    13021 agaacgggta tacggccgtg cggatcaccg aaatgaatga gaatttcaat caccagcatc
    13081 aggatgtgcg gacggataag ggggtagttt atggggatct ggcggagttc atggacttcg
    13141 aatatttacg gaagaatacg gcaatgaacc tggccaattt agggaacctg gctaaatcgc
    13201 cggggatgcc gcaggatgtt cgaatagagg tgcgggggtt aggtaatggg acttcattgt
    13261 cctggaaggc gccggttgac gggaagccta agggttattt tgtactgata cgggagacta
    13321 cgagtgcggt ttgggagaag aagatgtttt ttgcggggac ggaggcgaat ttgccgtatt
    13381 ctaaggacaa ttactttttt gccgtgcagt cggtgtcgga ggaggggaat gagagtttgc
    13441 cggtggtgcc ggtgccaggg cgctagtgta actttttcgg gggtgggttc gtcaaagggg
    13501 aaatacgtct accatgatac gcaactactt caagacggct atccgtaacc tttggaaaag
    13561 caaggggttt tcggcgatca atattatcgg tctggcgatc gggctggcta cctgtctgct
    13621 catcctgatc tttgtgatgg atgagctggg gtatgatcgg tataacgaaa aggcggatcg
    13681 tatctaccgg gtagatggcg acatcaaatt cgggggcaat cattttatcc tggcggtggc
    13741 gccggccccg gcggggccgg cgcttttgcg tgactaccct gaa
//
LOCUS       DASYSV010000557         6738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_884766, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000557 DASYSV010000000
VERSION     DASYSV010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884766"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1131)
                     /locus_tag="VGQ51_17390"
     CDS             complement(94..1131)
                     /locus_tag="VGQ51_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEV9038416.1"
                     /translation="MRGLKYILWLSGYLILVTYANGQDLHFSQWFNAPLLTNPANTGF
                     IPDADYRLGANYRNQWSAIMAQPYRTMSIWGDAQVFRNRIENGWMGLGGVILRDDAGT
                     STLTSTSVYGSVAYHQMVGYASLITVGFNTGWVNKRINTANLKFPDQFDGKFFDNTLP
                     TSVVIDHPSDNFFDMQIGVNYAYFPTNKMYLNAGFSVQHINTPRESFFNTDPVGYDDR
                     IARRYIGFFNASIKANDQVIINPMAYFTSMAGAQELSVGMSAQYNLQDEGDQQVIGGV
                     FYRMGDAIIPMIGFVYKNIRLTFTYDITTSALKNYDGGYGAYEFAIISQGFYSQYNGD
                     KRQSLCPSFKQ"
     gene            complement(1178..4852)
                     /locus_tag="VGQ51_17395"
     CDS             complement(1178..4852)
                     /locus_tag="VGQ51_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEV9038417.1"
                     /translation="MKKGSCFIIGLLIVALKGFAQDGASNIEFVENKGQWDPRVQFKG
                     DIPNGALFLEKAGFRVLLYNTDDLTAMTAAHHGVMSGTGPYRGGPTAKAGGSGIVAGK
                     PGMVRAHAYEVVFVNANAGVQIVPDKPLPGTNNYLIGPDTSTWGRDCKVFQGVTYRNI
                     YPNIDVRYYTNNGQLKYDIIVHPGGDISRVRMQYTGADKLSTHRGQLLVGTSVGTVTQ
                     LAPVSYLSNEAGRTDIRCRYSVHNGNTVSFDVPEYDGGATLVIDPVIVFCSFTGSKIS
                     NWGFTATPGPDGSFFAGGIVFGSAFPYNTGAIQPIYGGGKFDVGLIKLNSNGSTKIFA
                     TYLGGEDSETPHSLICDGNSEVIVLGRTYSGASFTHSTLIGPGGGADMFVVKLNAAGN
                     GVIGAVRIGGSQDDCVNMEDQARVSYERADSLIRNYGDDSRSEVVLDGAGNILVGASS
                     QSADFPIRGSVFQPVFGGGHQDGVVLELNPGCNNLVWASYLGGSGADACFVLNSDPMT
                     GDIYVGGATTSSDLPGNKAGVIQPSYGGGGCDGYVAQISSNGSTLKRSTYLGTNVSDA
                     VYGLKFDRKGFPYVMGTTNGTWPVLNAAYSVGGGRQFISKLQPDLSGFVYSTVFGTPS
                     GQAHGLPNISPVAFLVDRCENVYVSGWGGWIIGGESDPYGLLGTAGMPTTPDATKPVS
                     DNRDFYFIVIKKNASALLYGTFFGENDNSRSISEHVDGGTSRYDQQGVIYQAICGNCS
                     GPQATGRYPTTPGAWAPNNGAGNAGCNEVAVKIAFNFAGVAAGLRASVNGRLGDTAGC
                     IPMDTQLQDTIRNAKSYIWNFGDGSPDTATTGFAVTHTYPNPGVYTVMMVAIDSNTCN
                     VADTAYRHVIARTDRAQVDFSFIKDPAAPCTSLDFDFTNTSVPPAGKPFGASSFTWDF
                     GDNTAQVVNGPGPLTHSFASPGTYVINLILDDTSYCNFPDTASHTLRVSPLAKAQFET
                     PSTGCAPYPAYFNNTSLGGVTFNWDFGDPSSGAANNSTDVNPVHVYQNPGTYTITLQE
                     QDPSTCNKVSDTSFTITVSVKPVAAFTFSPSPPVANIPIVFTNGSEGGVSYKWMFGDG
                     DSLVTNSMDPVKYLYNSTDTFPACLLTTNQFGCTDTVCHPVATLINPLLDVPNAFTPG
                     RFGTNSILKVVGFGILRMDFRIYNRWGKMIFESDNPNVGWDGNYLGAPQPMDVYAYTL
                     EAEFSNGVHISKKGDITLVR"
     gene            complement(4865..>6738)
                     /locus_tag="VGQ51_17400"
     CDS             complement(4865..>6738)
                     /locus_tag="VGQ51_17400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEV9038418.1"
                     /translation="NAGGNQANGKQFITKLKQDLSGVVYSANFGPAGTAHPQISPTAF
                     LVDRCENVYVAGWGGGPDSTDHYANSTTNGLIVTAGAFKTATDGEDFYFFVLQKNAAS
                     QLFGSFFGQQYGNFGDHVDGGTSRFDKQGVIYEAVCANCYGGAQFPTTPGAYAQLNGT
                     GTGACNEAAVKIAFNFAGVAAGLKVVTHGRGDSVGCVPLPVSFSDTIHNAKSYIWNFG
                     DGTVVSGNNSIFSMNHTYVNTGTYVVTLIAIDSNTCNIADTVTRTVLVGNNPASVDFD
                     FSKLAPCTSMSYIFTNQSTAPPAVPFSGVAFTWNFGDGATAPNVPLGDQPTHAYASPG
                     PYNVTLTLNDTAYCNYPLDTVRQLYVAQNVLAKLNTPAIGCVPDSAQFTNNSIAGQQF
                     YWNFGDPASGVSDTSTAVNPVHLYTNVGTYQVTMIAVDSTTCNISDTTTYTVTMMGKP
                     TAGFTFSPEPPQPPNTPTVFTDASSPAVKYQWFFGDGTSETKTSPDTVVHQYIRTDTF
                     QVCLVVTNPSGCMDTACHVVPAVINPLLDVPNAFTPGRFGQNAVVKVIGFGITHMDWR
                     IYNRWGQLVFESSDPYAGWDGTYHGVLQPMDVYAYTLEAEYSDGTHSSKKGDITLIR"
BASE COUNT         1553 a         2021 c         1657 g         1507 t
ORIGIN      
        1 aagtcccgtc atccatagac cacacacgag tacaaacttt tttgtcatta gtcgatatga
       61 tttaggtagc gcaaaaatag ggaaataccg gcatcattgt ttgaaggaag gacacagcga
      121 ttgccgcttg tcgccattat actggctgta gaacccctgg ctgataatgg cgaattcata
      181 cgccccatac ccaccgtcat aattcttcag cgccgaggtg gtgatatcat aagtgaaggt
      241 tagccggata ttcttgtata caaacccgat catcgggatg atcgcatccc ccattctgta
      301 gaacactccc ccgatcacct gctgatctcc ctcatcctgc aggttgtatt gcgcgctcat
      361 ccccacagac aactcctgtg cgcccgccat cgaggtgaaa taggccatgg gattgatgat
      421 cacctggtcg ttggccttta tcgaagcatt gaagaacccg atatacctgc gcgcgatccg
      481 gtcatcatac cctaccgggt ccgtattgaa aaaactctcc cgcggagtgt tgatatgctg
      541 caccgaaaaa cccgcattca gatacatctt gttcgtggga aaatacgcgt agttcacccc
      601 gatctgcata tcgaaaaaat tgtccgatgg atgatcgatg acgacactcg tcggtaacgt
      661 attgtcgaag aatttcccgt cgaactgatc tggaaatttc agattggcag tgttgatccg
      721 cttgttcacc caccccgtat tgaaccccac cgtgatcaga cttgcataac ccaccatctg
      781 atgataggcg accgatccgt ataccgaggt ggaagtcagc gtgctggtac ccgcatcgtc
      841 cctcaggatc acgcccccaa gccccatcca gccgttttcg atccggttcc ggaagacctg
      901 ggcatccccc cagatactca tggtgcgata aggttgcgcc atgatcgccg accactggtt
      961 gcggtaattg gcccccagac ggtagtccgc atcgggtatg aatcccgtat tggccggatt
     1021 cgtcagtaag ggcgcattga accactgtga aaaatgcagg tcctgcccat tcgcataggt
     1081 caccaggatt aagtatccgc tcagccacaa tatatatttc aatcccctca ttttttgtag
     1141 taacggtgga tcggcgattt tggttgtctt caagcaacta tcttaccaaa gttatatctc
     1201 cttttttact aatatgcacg ccattggaaa attccgcttc gagggtatag gcatatacat
     1261 ccataggctg cggcgccccc agataattgc cgtcccaacc gacattcggg ttgtccgatt
     1321 cgaagatcat cttcccccac cggttataga tccggaaatc catccgtaag ataccaaaac
     1381 ctaccacttt caggatcgaa ttcgtaccaa acctgcccgg cgtaaaggca ttcggcacat
     1441 ccagcaacgg gttgatcagc gtcgccacgg gatgacagac cgtatcggta caaccaaact
     1501 gattcgtcgt cagcagacat gccgggaagg tgtccgtcga gttgtaaaga tattttaccg
     1561 ggtccatcga gttggtgacc agagaatccc catccccgaa catccatttg tagctgacgc
     1621 caccctccga tccgttcgtg aacacgatcg gtatattcgc cacaggcggt gacggagaga
     1681 aggtaaatgc cgccacaggc ttgacactca cggtgatggt aaaactcgta tcgctcactt
     1741 tattacacgt gctcggatcc tgctcctgca gggtgattgt ataggtaccc ggattctgat
     1801 agacatgcac ggggttcaca tcggtggaat tgttcgcggc tccgctcgac ggatcaccga
     1861 aatcccagtt aaaggtcacc cctcccaacg aggtattgtt gaagtaggcc ggataaggag
     1921 cgcatccggt ggatggcgtt tcgaattgcg ccttcgccag cggcgatacc cgcagggtat
     1981 gactggccgt atccgggaaa ttgcaataag aagtgtcatc gaggatcagg ttgatgacat
     2041 aggtcccggg actggcaaaa gaatgggtca gcggacccgg cccgttcacg acctgcgccg
     2101 tattatcccc gaaatcccag gtgaaggagg aggctccaaa cggctttccg gcaggcggca
     2161 ccgaagtatt ggtaaaatcg aaatccagcg aggtacatgg cgcggcagga tcctttatga
     2221 agctgaaatc cacctgggcc ctgtccgtcc gggcgatgac atgacgatac gctgtatcgg
     2281 cgacgttgca ggtatttgag tctatggcca ccatcatgac ggtatagacg cccgggttcg
     2341 gataagtatg cgtgacggca aagcctgtag tggcggtatc gggactacca tccccgaaat
     2401 tccagatata ggacttggca ttccgtatcg tatcctgcag ttgcgtatcc atcggtatac
     2461 agccggctgt atcccccagc cgcccgttga cggatgcccg cagtccggcg gcgacacccg
     2521 cgaaattgaa ggcgatcttg accgccactt cattacaacc cgcatttcct gcaccgttgt
     2581 tcggcgccca ggcgcccggc gtggtaggat aacgacccgt cgcctgcgga ccgctgcaat
     2641 tgccgcagat ggcctggtaa atgaccccct gctgatcata ccggctggtg cccccgtcga
     2701 cgtgttcact tatgctccgg gagttgtcat tctcgccaaa gaatgtccca tataacagtg
     2761 ccgaggcgtt cttcttgatg acgataaaat aaaaatcgcg gttatcgctc accggtttgg
     2821 tggcatcggg agtagtcggc atccccgcgg tccccaacaa tccataaggg tcggattcgc
     2881 cgccaatgat ccatccaccc catccggata catagacatt ctcacaccgg tctaccagga
     2941 acgccacggg cgatatattc ggcaacccat gcgcctgccc cgacggcgtt ccaaaaactg
     3001 tcgaatacac aaatccgctc agatccggtt gcagcttcga gatgaactga cggccgcctc
     3061 ctacggaata agcggcattc agcaccggcc acgtgccatt cgtggtcccc attacatacg
     3121 ggaacccctt tctatcgaac ttcaaaccat atacggcgtc gctgacattc gttccgagat
     3181 aggtcgaccg cttcaacgtc gacccgttcg atgagatctg tgcaacatat ccatcacaac
     3241 ccccaccccc atacgaaggt tggatcactc cggctttatt acccggaaga tcactcgagg
     3301 tcgtggcccc tcccacatag atatctccgg tcatcggatc gctgttgagt acgaaacagg
     3361 cgtcggcacc cgatccaccc agataacttg cccagaccaa attgttgcaa ccgggattca
     3421 gctcgagtac gaccccatcc tgatgaccgc cgccgaacac cggctgaaag acactccccc
     3481 ttatcggaaa atccgccgac tgcgacgagg cccctacaag aatattgccc gctccatcca
     3541 gtacgacctc actcctcgag tcgtccccat agttgcggat cagcgagtcg gctctctcat
     3601 agctaaccct cgcctgatct tccatgttga cgcaatcatc ctggcttccg ccgatccgca
     3661 cggccccgat cacaccattc cccgctgcat tcagcttcac tacgaacata tccgctcctc
     3721 cacccggccc gatcagggtg ctatgcgtaa agctcgctcc cgaataagtc ctcccgagta
     3781 cgatcacttc actgttgccg tcgcagatca ggctatgcgg ggtctccgag tcctcacctc
     3841 ccaggtaggt tgcaaaaatt ttcgtgctgc cattggaatt cagcttgatc aacccgacat
     3901 cgaatttgcc gccgccatat atcggctgaa tggccccggt gttataagga aatgcactcc
     3961 cgaagacgat gccaccggca aaaaagctgc cgtccggtcc cggcgttgca gtaaaccccc
     4021 agttgctgat cttgctgccc gtaaagctgc aaaaaacgat caccgggtcg atgaccaggg
     4081 tggcgcctcc atcgtactcc ggaacatcga aactcacggt attcccgttg tgtaccgaat
     4141 accggcaacg gatatccgtc ctccccgctt cattggaaag ataagagacg ggcgccagct
     4201 gggtgacagt cccgaccgag gtgcctacga gcagctgccc cctgtgcgtg ctcagtttgt
     4261 ccgctcccgt atactgcatc cgtacccggc ttatgtcccc gccgggatgg acgatgatgt
     4321 cgtatttcag ctggccattg ttggtatagt aacgtacatc gatattcgga tagatgttcc
     4381 ggtaagtgac gccctggaat accttgcagt cccttcccca tgtgctggta tccgggccga
     4441 tcaggtagtt gttggttccc ggcagaggct tgtccggcac gatctggacg cccgcattgg
     4501 cgttcacgaa tacgacctca tacgcatgcg cccgcaccat tccaggtttc cctgccacga
     4561 tacccgaccc gccggctttt gccgtcggcc ctccccggta aggacccgtc ccgctcatca
     4621 ctccatgatg cgcggccgtc atcgccgtca gatcgtccgt attgtacaat aataccctga
     4681 accccgcctt ctcaaggaat aatgcgccgt tcggaatatc ccccttgaat tgcacacggg
     4741 ggtcccactg acccttattc tccacgaatt caatattgga cgcgccatcc tgggcgaacc
     4801 ccttcagcgc gacgatcagc aacccgatga tgaaacacga ccccttcttc atagatagca
     4861 tagattacct gattaacgta atatctccct tttttgatga gtgcgttccg tccgaatact
     4921 ccgcttccag cgtataggca taaacatcca tcggctggag gactccatga tatgttccgt
     4981 cccaaccggc ataaggatcg gagctctcga acaccagctg accccaccgg ttgtaaatac
     5041 gccaatccat atgcgttatg ccgaacccga tcactttcac caccgcattc tgaccaaatc
     5101 gcccgggcgt aaaggcattc ggcacatcca gcaacggatt gatcaccgcg ggtacgacat
     5161 gacaggcggt atccatacaa ccggatggat tggtgaccac cagacacacc tggaatgtat
     5221 ccgtccggat atactgatgc acgacggtgt ccggcgaagt cttcgtctcg ctcgttccgt
     5281 cgccgaagaa ccattggtac ttcactgcag gcgatgaggc atccgtaaag accgtcggcg
     5341 tattcggcgg ttgcggaggc tcagggctga aggtgaatcc cgcggtgggc ttgcccatca
     5401 tggtcacggt ataggtcgtc gtgtcggaga tattgcaggt cgtcgaatcc acggcgatca
     5461 tggtcacctg gtaagtcccc acattggtat acaaatgaac gggattgacg gcagtggacg
     5521 tatcgctcac gccgctggcc gggtctccga agttccagta gaattgctgc cccgcaatgg
     5581 agttattggt gaactgcgca gaatccggaa cacatcctat cgccggcgta ttcaacttcg
     5641 ccagcacatt ctgcgcgaca tataattgcc tgacggtgtc caacggatag ttgcaatagg
     5701 cggtgtcgtt cagcgtaagt gtcacgttgt acggacccgg agaagcatat gcatgcgtcg
     5761 gctgatcccc cagcggaaca tttggcgcgg tggcaccatc cccgaaattc cacgtgaagg
     5821 ccacaccact gaacggcacc gctggcggcg ccgtcgactg attggtaaaa atatagctca
     5881 tcgaggtaca cggagctaat ttggaaaaat cgaaatccac cgaggccggg ttattcccca
     5941 ccagcacggt tcgcgtcacg gtatcggcga tattacaggt attactgtcg atggcgatca
     6001 gcgtcaccac ataggtaccc gtattcacat aggtgtggtt catcgagaag atactgttat
     6061 taccgctgac gaccgtaccg tccccgaaat tccagatata tgatttggcg ttgtgtatgg
     6121 tgtccgaaaa ggacacaggc agaggcacac accctacgct gtcgccacgg ccatgcgtaa
     6181 ccactttcaa ccccgccgca actccggcga aattgaaagc gatcttaacg gcagcttcat
     6241 tgcacgcacc cgttcccgtc ccgttcagct gcgcataggc cccgggagtc gtcgggaact
     6301 gcgccccgcc ataacaattc gcacacacgg cttcatagat taccccctgt ttgtcgaacc
     6361 ggctcgtccc gccatccaca tgatccccga aattgccata ctgctgtccg aagaaactgc
     6421 cgaatagctg agacgccgca ttcttctgca aaacgaaaaa ataaaagtcc tccccgtccg
     6481 tcgctgtctt gaatgcacca gccgtcacaa ttagcccatt ggtggtgctg ttggcataat
     6541 gatccgtact gtcgggtccc cctccccagc cggctacata cacgttctcg cagcgatcca
     6601 ccaggaaggc tgtcggagat atctgcggat gagccgtccc cgccggtccg aagttggcag
     6661 aataaacgac cccagacagg tcctgcttca gtttggtgat gaattgtttt ccattcgcct
     6721 gattcccgcc cgcattga
//
LOCUS       DASYSV010000558         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2293338, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000558 DASYSV010000000
VERSION     DASYSV010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293338"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VGQ51_17405"
     CDS             complement(<1..514)
                     /locus_tag="VGQ51_17405"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV9038419.1"
                     /translation="MDIVSSLFVIIFVYPWLFPILFVLIRLDSRGPVFFRQQRVGFLG
                     KTFWCYKFRTMHVNNYADTRRASSDDPRVTRVGRFLRNTCLDEFPQFINVLLGHMSIV
                     GPRPHMLHDSREFASLIDNYRFRDMVRPGITGLSQVRGYRGPAANFESILRRYQWDSF
                     YVRNTGFLLDL"
     gene            complement(942..1706)
                     /locus_tag="VGQ51_17410"
     CDS             complement(942..1706)
                     /locus_tag="VGQ51_17410"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV9038420.1"
                     /translation="MVEPGSIVKNLQVEQVGEKRSVNLRAFISDRRSYLLLKRIFDIG
                     ISLFALVFIFSWLFPIVWLLIRIDSKGPVFFIQKRVGFLGRTFRCLKFRTMHVNAEAN
                     TKQATDNDSRITRVGHFLRRSNLDELPQFLNVLAGHMSVVGPRPHMYRDCDNFSNVVE
                     GYKFRNLMKPGITGLAQVKGYRGPAQTFDKIFRRYQWDAFYVRNAGLWLDLRIIHYTV
                     MQTFSYLISKFIVIEDGPIESLKSDWVELEPKNVLN"
     gene            2072..3385
                     /locus_tag="VGQ51_17415"
     CDS             2072..3385
                     /locus_tag="VGQ51_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038421.1"
                     /translation="MTLLAKKPVFVVTLAVLFTTLFSWFQTNSWCIILLLICGLWVEG
                     PVKAIRTAFTNKYFLAYFIYWLIQAAGLFYTHNPVKGQNLVAKDTTLVAIAFVLCGCR
                     FADEKGYRSLITAYSLLVFASALYCLYRAEILYVRLHDSSVFFYHPLAAPLSQNAVFY
                     SVYVLFAMQFLLSPEGDPAIGLSAPVRKWCRILLVVFFLGMIVLLSSKLILILALLML
                     VNFFGKRYYTTQNLKALVISGLAAICLVAGLFAIDNPVKQRFTKMVDGGISVPKRDTI
                     NAATAFDPLPLRILEWHFAFEILREHHAWLFGVSPGDSQDLLDQKYVDTHMWIGNPDD
                     GPDRRIRGFLGYNFHDQYIETLVRSGLIGFASLMLILGLQIGIALKWRTKEARFTVLT
                     LILFFIPESPLTMQHGVFLFCFFPLLLLYSRRKVPIMVVKQPDKS"
     gene            3465..>4216
                     /locus_tag="VGQ51_17420"
     CDS             3465..>4216
                     /locus_tag="VGQ51_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038422.1"
                     /translation="MSEKNIHPEDQIDLSGAKAVFYGLLRAFFKALSFTWYVLRTNKY
                     IITIFLVLGMGFGYLYYANKPSHYKAMMIVRYNMLTKKTYAEMIEQLNHLIATAPKAR
                     LARELRVPENMADKLLHVDARDMNDVYLANDTSTKVDQPFKIIASLRDDYDFALDSLQ
                     DGVLRYLNGSPYLKRLKEEQHELERFRLQTLDNDLGKLDTLKTEYNHFLASSKMSATF
                     YNNASNPADFYNQTSTLLAQRELTLTSLRVDS"
BASE COUNT         1091 a         1036 c         1038 g         1051 t
ORIGIN      
        1 gcagatcgag caggaagccc gtattccgga catagaatga atcccattgg tagcgacgca
       61 ggatgctctc gaaattggcg gcggggcccc ggtagcctct cacctgcgaa agcccggtga
      121 tgccgggtcg gaccatgtcg cgaaaccggt agttgtcgat gaggctcgca aattctctcg
      181 aatcatgaag catatggggt ctcggcccta cgatactcat gtgtccgagc aggacgttga
      241 tgaattgggg aaactcatcc aaacaggtat tgcggaggaa acggccaacc cgtgtcacac
      301 ggggatcatc ggacgaagcc cggcgggtgt cagcataatt gtttacatgc atggtccgga
      361 acttataaca ccaaaaggtc tttcccagga atccaaccct ttgctggcgg aaaaagaccg
      421 gtcccctgga atccagccgg atcagaacga agaggatggg gaataaccag ggatagacga
      481 aaatgatcac gaataaagat gaaacgatat ccattatccg cttgtaaatc agataattat
      541 acctttcacc ccaatattcc cgaacaaatt cccagttccc tttttcccag ttttcaggac
      601 aacgaatgga cgattcaaca aatgtgctct gcattttact caggattggt tttcgacttc
      661 ttttccttct atacgagcgg acggtcgaca agatgcaaaa aaactgttaa tacatatatg
      721 aattttcgag ggtatccgca cgaattgaca atgtcgactg taaaaacgtt gcattttaac
      781 gcgttaatta aataagacca aatcgagcca ggcgcccgaa ggggtctcac aaaccgcgga
      841 ttgcacagtt atacgtcaaa tcgacacgat tatcgcccga atcgacacag ttatacaaga
      901 attaaaaaga ggttaaaaaa tagttaagat ttcaatcagg atcagttcag cacatttttg
      961 ggctcgagct cgacccaatc gcttttaagg gattcgatgg ggccgtcttc gatgacgatg
     1021 aatttactga tcaggtagga gaatgtctgc atgacggtat agtggatgat ccgcaggtcg
     1081 agccagaggc cggcattacg gacatagaag gcatcccatt ggtatctccg gaagatcttg
     1141 tcgaaggtct gggcgggacc acggtagcct ttgacctggg cgaggccggt aatgcccggt
     1201 ttcatcagat tgcgaaactt gtatccttcg acgacattgg agaaattgtc gcaatccctg
     1261 tacatatggg gacggggtcc caccacactc atatgcccgg caagaacatt gaggaactgg
     1321 ggaagctcgt cgagattcga acggcgcagg aaatgtccca cgcgggtgat ccgggagtca
     1381 ttgtccgtag cctgtttggt attggcttcg gcattgacat gcatggtcct gaatttaagg
     1441 caccggaacg tccgacccaa aaagccgacc cgcttctgaa tgaagaatac ggggcccttt
     1501 gaatcgatac gtatgagtaa ccaaacaatg gggaaaagcc aactgaaaat gaagaccagc
     1561 gcaaatagtg atatcccgat atcgaatata cgtttcagaa ggaggtagga ccggcgatca
     1621 ctgatgaaag cccttaggtt aaccgacctt ttttcgccaa cttgttctac ttgcaaattc
     1681 tttacgatgc ttccgggttc gaccataaga ccgaaggttt aatatataat ttttagaaaa
     1741 ccccacctat aacaactttt catacgactt attgccggga aaagagctaa atttttccat
     1801 ttggcagatc accgcatatc agggaaataa cagaatccgc taaagaggta gttgacggct
     1861 ccgaaatgat ttccggacaa cagcggggtt ctttagcaat caggttgaat ttcaagtaaa
     1921 taaattacct cgccgcaaag taacgaaaaa aaatcctttc aaaaaatacc actttgcggt
     1981 aactgttaaa tctttcaaaa gaaatatatt ttggttataa aacagtgctc gactattatt
     2041 gcaccatggc atcgaacaaa atgtcggtct tatgacttta ttggccaaaa agcccgtgtt
     2101 tgtcgttacc ctagcggttc ttttcactac tttgttctcc tggtttcaga ccaatagctg
     2161 gtgtatcatt cttctgctga tttgcggatt atgggtggaa ggtccggtaa aagccatcag
     2221 gacggccttc acgaataaat atttccttgc atatttcatc tattggctaa tacaggcagc
     2281 cggtctgttt tatacgcata acccggtaaa agggcaaaac ctggtcgcca aggacaccac
     2341 cctggtcgcc atcgcctttg tactctgcgg ttgccggttt gccgacgaaa agggttaccg
     2401 gagcctgatc acagcctact ccctgctggt ctttgcctcg gcgctttatt gtctgtaccg
     2461 ggcggagatc ctctatgtcc ggctgcatga cagctcggtc tttttttatc acccactggc
     2521 cgcccccctt tcccaaaatg cggtctttta ttcggtatat gtccttttcg ccatgcaatt
     2581 ccttttgtcg ccggaaggcg acccggccat cggcctgtcc gcgccggtaa ggaagtggtg
     2641 ccggatcctg ctcgtcgtct tctttctggg aatgatcgtt ttattgtctt cgaaactgat
     2701 actgatcctt gcactcctca tgctggtcaa ttttttcggc aagagatact ataccactca
     2761 gaatctgaag gcattggtga tatcgggtct tgcggccatc tgcctggtcg ccggtctttt
     2821 tgccatcgat aacccggtga agcaacgatt tacgaagatg gtcgacggcg gtatcagcgt
     2881 tcccaaaagg gacacgatca atgccgcgac ggcattcgac cctcttccgc tgagaatcct
     2941 ggaatggcat ttcgccttcg agatcctgag agaacatcat gcctggcttt tcggggtgag
     3001 tcccggtgac agccaggatc tgctggatca aaaatatgtc gatacgcaca tgtggatcgg
     3061 caaccctgac gacggaccgg accggagaat aagaggattt ctcggataca atttccatga
     3121 ccagtatatt gaaaccctgg tccgttcggg cttgatcggg tttgcgagcc tgatgctgat
     3181 cctcggcctg cagataggta tagcactgaa atggaggacg aaagaggcga ggtttaccgt
     3241 cctgacgctg atcctgttct ttattccgga gtctccgctc accatgcagc atggggtctt
     3301 tcttttctgt tttttccctc tgctcctgct ctacagccgc cgaaaagtcc cgataatggt
     3361 tgtaaaacag ccggataagt cctaatattg caccgtaaaa aaacgcggga ggccgtgact
     3421 gtcgcgaccc gcagcaaacc aagagccaag ccgatcgttt caatatgtct gaaaaaaata
     3481 tccatccgga agaccaaatc gacctctccg gggccaaggc cgttttctac ggtcttttgc
     3541 gcgccttctt caaggcgctg tccttcacct ggtatgtcct tcgcaccaac aagtacatca
     3601 tcaccatctt cctggtgctc ggaatggggt ttggatatct ttattatgca aataagcctt
     3661 cgcattacaa ggcgatgatg atcgtccggt acaacatgct gaccaagaaa acgtatgcgg
     3721 agatgatcga gcagttgaac cacctgatcg ccacggcgcc caaagccagg ctggcgcggg
     3781 agttgcgtgt gccggaaaat atggccgaca agttgcttca tgtggacgcg cgtgatatga
     3841 acgatgtcta cctggcgaat gatacttcca ccaaggtgga tcagccgttc aagataatcg
     3901 cgagtctccg ggatgactac gatttcgcgc tggacagctt gcaggacggg gtcttgagat
     3961 acctgaacgg ctctccctat ttaaagaggc tcaaggagga gcagcacgag ctggagcggt
     4021 tcaggctgca aaccctcgac aacgatctgg gtaagctgga tacgctgaag acggaataca
     4081 accatttcct cgcctcttcg aagatgtcgg cgactttcta taacaatgcc agcaatccgg
     4141 cagattttta taaccagacc tcgaccttgc tggcccagcg cgaattgacg ctgacgtcat
     4201 tgagggttga cagttt
//
LOCUS       DASYSV010000559         6644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1396933, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000559 DASYSV010000000
VERSION     DASYSV010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396933"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="VGQ51_17425"
     CDS             complement(<1..1483)
                     /locus_tag="VGQ51_17425"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV9038423.1"
                     /translation="MIKLSLFLLSACFFVPVALRAQQEQIDTSMVNRIRQEEAMNSQI
                     PYIAHEITDVAGARLTNSPGWHHAADWVVQTLKSWGFANAQKEPWGQFGYGWTAEKTT
                     VALKTPYYSPLIAYAIPWSGSTHGLITAPVFLIRKMDSVWIAGHLQEMKGKIVLIAPQ
                     DTTDPPRFKPDAERYSDSALAAIGDMYMVNRAMIQMYVPYLTKMMHLSKMIGGSGAVA
                     TLGTGGGRDGTVFVQSFVGYRKKDQPMIPDIELSKEDFLRVMRLAEDGQSPTIELQSE
                     TRLSDQDLQGHNVIAEIPGTDPTLGSQVVMLGGHLDSWTAGTGATDNGAGCIVALEAV
                     RLLKTLGVKPKRTIRIALWDGEEEGLLGSFHYVRNHFGDPSDMKLKPGQRQVSVYFNL
                     DNGSGRIRGIFAQGNDQAAAIFRQWLVPFRDMGASTVTMHNTGSTDHLSFDAVGIPGF
                     QFIQDPLDYESRTHHSNQDNYDHLEMGDLRQAAVIEASFVLFAA"
     gene            1540..2655
                     /gene="recF"
                     /locus_tag="VGQ51_17430"
     CDS             1540..2655
                     /gene="recF"
                     /locus_tag="VGQ51_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218293.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEV9038424.1"
                     /translation="MTSLKEISLVQFKNYTQRSFAFGKRIVGICGNNGVGKTNLLDAI
                     YYLCFTKSYFSRSDQTSVLRGSSGFRIEGQFDQDGAAINVVCILRPTGKKEFLADGIP
                     YEKFSRHIGRLPCVIIAPDDIQIVTGGSEERRRFLDALLSQLDPAYLQDLIEYNKVLQ
                     QRNGFLKTLTERRLTDKGLLDVYDEQLAVRGTRLFERRVAFLKELLPLAGSFYAQIAG
                     AEEPLALAYNSQLFHSSFRELLRQGLEKDLYLQRTGSGIHRDDIDISFAGVPFKTMAS
                     QGQRKSLLFALRLSEYEILRREKGFPPMLLLDDVFEKLDENRMNNLLDKVCLQGDGQI
                     FITDTHPGRISREMERIGADWQLITLSQEGLPIAGTP"
     gene            2749..3189
                     /locus_tag="VGQ51_17435"
     CDS             2749..3189
                     /locus_tag="VGQ51_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038425.1"
                     /translation="MSPLESSIHYLEETNDPADAFFSYTVDDTGEYGLIKANKEGLRL
                     YAAEILKKSKELEETEHAERPMFFSPRQWMFSETGYDLIAGVLPQYHSRREILTDHPG
                     SRRNSSGRRRQPVLLFVLMCITGALIMLATLKAFPNLRLWVNIR"
     gene            3171..3452
                     /locus_tag="VGQ51_17440"
     CDS             3171..3452
                     /locus_tag="VGQ51_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEV9038426.1"
                     /translation="MGEYSIGDAMRKFLHGSQLKGSIQALQIEEVWETLMGKTVARYT
                     DRIRIHGQTLYVETSIAPLRQELIYQKETIIQRVNEAMGEKVVREVVIK"
     gene            complement(3517..4566)
                     /locus_tag="VGQ51_17445"
     CDS             complement(3517..4566)
                     /locus_tag="VGQ51_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038427.1"
                     /translation="MLRYLIRRSFYGVLVMLGVVVTVFFLFQGFGDPSRLIIGQRADA
                     ATQDNIRKELYLDKPKWKQFVLYCNDILPIGIHTRSEIREKGLRGVFIGGNTTIGIKW
                     PYLRRSYQSKKEVWDILMEALPGTLLLATAAMVLATILGIGLGVLAAIKKDTWMDTSS
                     IFVSVLGISAPSFFMGIVIAYLFGFVLSGVTGLHMTGSLFDTDPFLGRQLQLRNLILP
                     ALTLGIRPMAIIAQLTRGSLLDTLDQDYIRTAYAKGLPARTVVWKHALRNALNPVITA
                     ITGWFAELLAGAFFVETIFGWKGIGKVTVDALEQLDFPVVMGSVLVSSFFFVLVGLLS
                     DLLYGLIDPRVRVTG"
     gene            complement(4569..5720)
                     /locus_tag="VGQ51_17450"
     CDS             complement(4569..5720)
                     /locus_tag="VGQ51_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BT_3928 family protein"
                     /protein_id="HEV9038428.1"
                     /translation="MKTILSVIRAIVGILFIFSGLVKANDPLGLSYKMQEFFGVWNFH
                     LLDHLTLAFSILMIVFEIVAGVAVLLGWRMKLFSWLLLLLIIFFTFLTGYAYLTGKVR
                     ECGCFGDCIPLSAGESFAKDIILLVLILVLFALRSRIRPVASPTVSAALLTMTVVFSV
                     AFQWFVLIHLPYVDCLPYKRGNDLVEKMQSPPGSVPDSTVITLVYNKNGKQLEFGAGA
                     FPADFDSTYHFVNRYDKLVRKGNAEPPIHDFNLVTAGGTDTTQEVLAAKEYKLFLFLR
                     ELEEESPSWNKAFSIILTMTRAKRIPIYIITADFENVEAWTVRQGIANAVTVLKCDAT
                     AIKTAARANPTLYLIHGSTVKDKWSYADLDAALPSVNELGEQLAAPAQH"
     gene            complement(5732..6283)
                     /locus_tag="VGQ51_17455"
     CDS             complement(5732..6283)
                     /locus_tag="VGQ51_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEV9038429.1"
                     /translation="MKITEDQYDAAVRTCRGIFLQKTRDYGTSWRVLRTVSIVDQLYI
                     KARRIRTLQETKTGKVGEDKAGEFRAMVNYGVIGLIQLSIEPGVAEELTEIEASERYD
                     GKIALARETMLAKNHDYGEAWREMSQEGFTDLILMKLLRIRQIVANDGKTLISEGVDS
                     NYIDIINYSLFALILIDEGKHTQ"
     gene            6386..>6644
                     /locus_tag="VGQ51_17460"
     CDS             6386..>6644
                     /locus_tag="VGQ51_17460"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV9038430.1"
                     /translation="MFTLNSRGRLLLIDRPIIMGILNRTSDSFYSGSRVREDDALLEH
                     ADRMIGEGADWLDIGGQSTRPGAETVGAEEELQRIIGAIDLI"
BASE COUNT         1532 a         1847 c         1774 g         1491 t
ORIGIN      
        1 tcgccgcaaa aaggacgaaa gaagcctcta tgaccgcggc ctgacggagg tcccccattt
       61 ccaggtgatc ataattgtcc tggttgctgt gatgggtcct ggattcataa tccagcgggt
      121 cctgtatgaa ctggaagcca ggaataccca ccgcatcgaa gctcagatga tcggtcgaac
      181 ctgtattgtg catggtgacc gtggaagcac ccatgtccct gaacggtacg agccattgac
      241 ggaagatggc agccgcctgg tcgttaccct gtgcaaagat acccctgatc cgtcccgagc
      301 cattgtccag gttgaaataa acggagacct gacgctgccc tggtttgagc ttcatatccg
      361 acgggtcccc gaaatggttc ctgacatagt ggaacgagcc cagcaatccc tcttcctcgc
      421 catcccagag ggctatccgg atcgtccgct tcggtttgac ccccaacgtc ttcagcagtc
      481 tcaccgcttc cagggcgacg atacagcccg ccccattgtc cgtggcgccg gtgcctgccg
      541 tccaggagtc gagatgtccc cccagcatga ccacctgcga acccagcgtg gggtcggtgc
      601 cgggtatctc ggcgattacg ttatggccct gcagatcctg atcggataat cttgtttcgc
      661 tttgcagctc gatcgtcggc gactgcccgt cttcggccag ccgcatcacc ctcaggaaat
      721 cctctttaga caactcgata tccggtatca tgggctggtc ctttttgcga taacctacga
      781 agctctgcac gaatacggta ccatctcttc ctccccccgt gcccagggtt gcaaccgctc
      841 cgctgccccc gatcattttg gacaggtgca tcattttcgt cagatagggc acatacatct
      901 ggatcatcgc cctgttgacc atatacatgt ctccgatggc ggccagcgcg ctgtccgaat
      961 agcgttcggc gtccggtttg aaccgcggcg ggtccgtagt gtcctgcggc gcgatcagca
     1021 cgatctttcc tttcatttcc tgaagatggc cggcgatcca gacggaatcc attttacgga
     1081 tgagaaagac gggcgcggtg atcagaccat gggtgctgcc ggaccagggt atcgcatagg
     1141 cgatcagcgg gctgtaatag ggcgtcttca acgcgactgt ggtcttttcg gcagtccatc
     1201 cgtatccaaa ctggccccag ggttccttct gagcattggc aaacccccac gacttcaaag
     1261 tctgcacgac ccagtcggcg gcatgatgcc agccgggtga attggtcagg cgggctccgg
     1321 cgacatccgt tatctcgtgt gcaatatagg ggatctgact attcatcgcc tcttcctgcc
     1381 ggatgcggtt aaccatcgac gtgtcgatct gctcttgctg ggcgcggagc gctaccggca
     1441 cgaaaaagca tgccgatagc aggaataggg aaagtttaat cataacagcg tagtttaagt
     1501 ttaaaggaaa ggtaatattt tacacccgaa ttcagctcag tgacgagtct aaaagaaata
     1561 tcgcttgttc agttcaagaa ctatacgcag cgctcgttcg cgttcggcaa gcggatcgtc
     1621 ggcatttgcg gcaacaacgg ggtcggtaag accaatctgc tggatgctat ctattatctc
     1681 tgctttacga agagctattt ttcacgcagc gatcagacca gcgtcctgcg gggttcttcg
     1741 ggctttcgta tcgaagggca attcgaccag gacggcgccg ccatcaatgt cgtctgcatt
     1801 ttacgaccca ccggaaaaaa ggaattcctt gccgacggta tcccgtatga gaaattttcc
     1861 cggcatatcg gccggttgcc ctgtgtcatc atcgcgcccg atgacatcca gatcgtcacc
     1921 ggcggaagcg aggagcgccg gcgttttctc gatgcgctgc tctcgcagct ggacccggcg
     1981 tatctgcagg acctcatcga atataacaag gtgctccagc aacgaaacgg attcctcaag
     2041 acgctgacgg aaagaaggct gaccgacaaa ggtcttctgg acgtttatga tgagcagctg
     2101 gcggtgcgcg gtacccgtct tttcgaacgc cgcgtagcct tcctgaaaga gctcttaccc
     2161 ctggccggca gtttctatgc gcagatcgct ggcgccgagg agccgctggc cctggcctac
     2221 aatagccagc tcttccactc atcgtttcgc gagttgctcc ggcaggggct ggaaaaggat
     2281 ctatacctgc agcgtaccgg cagtggtatc caccgggacg atatcgatat cagttttgcc
     2341 ggagtgcctt ttaagaccat ggcttcacag ggacaacgca agagcctatt gtttgcgctg
     2401 cgtctttctg aatatgagat cctccggaga gaaaagggtt ttccgccgat gctgctcctg
     2461 gatgacgttt tcgaaaaact ggatgagaac cgcatgaaca acctgcttga caaggtctgt
     2521 ctgcagggcg acgggcagat ctttatcacc gatacgcacc cgggacggat cagccgggag
     2581 atggaaagga tcggggcgga ctggcagctg atcacgctga gccaggaggg tcttcccatt
     2641 gcgggaacgc cttaattttt ttacgttgaa aagcgggtag tcactaaaaa aaagttatat
     2701 ttattctcca aatttcaacg ccgtaaaaac gcccgtaaaa cgattgctat gtcccccctg
     2761 gaatcttcca ttcattacct tgaagaaacg aacgaccctg ccgacgcttt tttttcctac
     2821 acggtcgacg ataccggtga atatggtctg atcaaggcca ataaggaagg tcttcgtctg
     2881 tatgcagccg agatcctgaa aaagtcgaaa gagctggaag aaaccgagca tgccgaacgg
     2941 ccgatgttct tttcaccgcg acaatggatg ttcagcgaga ccgggtatga cctgatcgcg
     3001 ggtgtccttc cccaatatca tagtcgtcgc gaaatactta ccgatcatcc cggctcaagg
     3061 agaaattctt caggacgtcg ccgtcagccc gtactgcttt ttgtgctgat gtgcataact
     3121 ggcgccctca tcatgttggc tacattgaaa gcgtttccta atttgcgctt atgggtgaat
     3181 attcgatagg agatgccatg cggaaattcc tgcacggcag tcagctgaaa gggtccattc
     3241 aggccttgca gatagaagag gtctgggaaa ctttaatggg taaaaccgtg gcccggtaca
     3301 ccgatcgcat ccggatccat gggcaaacgc tctatgtcga gacctccatc gctcctctcc
     3361 gccaggagct tatttatcag aaagaaacca tcatccagcg cgtcaacgag gcgatgggcg
     3421 aaaaagtcgt ccgggaagtc gttatcaaat aggcattcgc gggattggcc ctccgtaggc
     3481 attcgcgaag ccctccgcgc gaaaattcac ggtcgcctac cctgttaccc gcaccctcgg
     3541 gtcgatcagc ccatagagga gatcggagag cagccccacc agtacaaaga aaaaagacga
     3601 caccagtacc gaccccatca ctacgggaaa atccagttgc tccagcgcgt cgacggtcac
     3661 tttcccgata cctttccacc cgaagatggt ctcgacgaaa aaagctcccg ccaacagctc
     3721 ggcaaaccaa cctgtgatcg cggtgatgac cggattcagc gcattgcgca acgcgtgttt
     3781 ccagacgacg gtcctggcgg gcaagccttt cgcataggcg gtacggatat agtcctgatc
     3841 gagggtatcc aggagcgagc cccgcgtcag ctgtgcgatg atcgccatcg gccggatgcc
     3901 cagtgtcagg gcgggcaata tgaggttccg cagctgcaac tgacggccaa gaaaggggtc
     3961 ggtatcgaaa aggctgccgg tcatgtggag accggtgacg ccgctgagga cgaatccgaa
     4021 aagataggcg atgacgatgc ccatgaaaaa cgagggcgcg gagatgccca gcacgctcac
     4081 gaaaatggac gaggtatcca tccatgtatc cttcttaata gccgccagca cgcccagacc
     4141 gatcccaaga atggtcgcga ggaccatggc cgcggtggcg agcagcagcg tgccgggaag
     4201 cgcctccatc aggatatccc atacttcttt tttggattga tacgaccggc ggagataggg
     4261 ccatttgata ccgatggtcg tgttcccccc gatgaatacg ccgcgcaaac ccttttcgcg
     4321 gatctcgctg cgggtgtgga tgcctatcgg caaaatatcg ttacaataaa gcacgaactg
     4381 tttccacttg ggcttatcga gatataattc cttccggatg ttgtcctggg tggccgcgtc
     4441 agcacgctgc ccgataatga gacgcgaggg gtcgccgaat ccctggaaca ggaaaaaaac
     4501 ggtaacaacc actcccagca tgaccagcac accgtaaaaa gatcgtcgta tgagatatcg
     4561 aagcatgttt aatgttgcgc cggggcggcc agttgttctc ccaattcatt caccgagggc
     4621 agcgcggcat cgagatcggc atagctccat ttatccttga ccgtgcttcc gtggataagg
     4681 tatagcgtag ggttggccct tgccgccgtc ttgatcgccg tggcatcgca tttgagcacc
     4741 gtaacggcat tcgcgatgcc ctgacggacg gtccaggctt ccacattctc aaaatcggca
     4801 gtgatgatat agatgggtat ccgcttggcc ctggtcatgg tcaggataat gctgaaagcc
     4861 ttattccagg aaggggattc ttcctccagt tccctcagga acaggaagag cttatattct
     4921 tttgcggcca gcacctcctg cgtggtatcc gttcccccgg cagtgacgag attgaagtca
     4981 tggatcggcg gttccgcatt cccttttcgc accagcttgt cataacgatt cacgaagtga
     5041 taggtgctgt cgaaatctgc cgggaatgca cctgccccga actccaattg ttttccattc
     5101 ttattataga cgagcgtgat gaccgtgctg tcgggtaccg agccgggcgg tgattgcatt
     5161 ttttcaacga gatcgttgcc ccttttataa ggaagacagt ccacatatgg caggtgtatc
     5221 aggacaaacc attgaaaggc gaccgaaaac acgaccgtca tcgtaagcag cgcggcgctg
     5281 acagtcggcg acgcgaccgg ccggatgcgg cttcgcaggg caaacaggac aaggatcagt
     5341 acgagcagga tgatgtcctt ggcgaacgac tcacccgccg agagtgggat acaatccccg
     5401 aaacagccgc attcgcgcac cttgccggtg agatacgcat aacccgtcag gaatgtaaag
     5461 aagatgatca ggagcaggag cagccagctg aacaatttca tcctccagcc cagcaggacg
     5521 gccacgcccg ccacgatctc gaaaacgatc ataaggatcg agaaggccag tgtcaaatgg
     5581 tccagcaaat ggaaattcca taccccgaag aactcctgca tcttatagct cagacccagc
     5641 ggatcgttgg cctttactaa tccggagaag atgaacagga tgcctacgat cgcccgtata
     5701 acggaaagga tggtcttcat gatacctgcg tttactgcgt atgtttgcct tcgtcgataa
     5761 ggatgagggc aaaaagggaa taattgatga tatcgatgta attggaatcg acgccttcgc
     5821 tgatcagggt cttcccgtca ttggcgacga tctgacgtat cctcagcagc ttcatcagga
     5881 tcaggtcggt aaagccttcc tggctcatct cccgccaggc ctcgccatag tcgtgatttt
     5941 tggcgagcat ggtctcccgt gccagcgcga tctttccgtc atagcgttca ctggcttcaa
     6001 tctccgtcaa ctcttcggca accccgggtt cgatggacag ctggataagc ccgataaccc
     6061 catagttgac catagcgcgg aattccccgg ctttatcctc tcctaccttt cccgtctttg
     6121 tctcctgtaa ggtccggatc cgtcgcgctt tgatatacag ctgatcgacg atggagaccg
     6181 tgcgcaatac ccgccaggaa gtgccataat cccgcgtctt ttgtaaaaaa atacctctgc
     6241 atgtccgcac cgccgcgtca tattgatctt ccgttatttt cataaatatg ttacaattga
     6301 taccagttct cttgctattt taacgccaat gtctgacctt cggtcgaccc gttttcaacg
     6361 gctccggatg caattataac caattatgtt cactttgaat agcagaggca ggctactcct
     6421 gatcgaccgc cccatcatca tgggcatcct gaacaggacg tcggattcct tttatagcgg
     6481 tagccgtgtc cgggaggatg acgcattgct ggagcacgcc gaccgcatga tcggcgaagg
     6541 tgcggactgg ctcgatatcg gcggtcagag cacccgtcca ggcgcggaga cggtgggggc
     6601 cgaagaagaa ttgcaaagga tcatcggcgc catcgatctc attc
//
LOCUS       DASYSV010000560         8649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_238868, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000560 DASYSV010000000
VERSION     DASYSV010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238868"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..555
                     /locus_tag="VGQ51_17465"
     CDS             151..555
                     /locus_tag="VGQ51_17465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9038431.1"
                     /translation="MTDTNNKVLIVDDELDICYLLSGMLKQRNFVTGFVNTLSDAANA
                     LQTDTPSLLFLDNHLPDGFGLDFIPYIKKNYPEVKVIMITAHDGAADRKQAYDGGVDL
                     FVAKPLNRKMINDAIDKLYSTDPAGHKAYRFQ"
     gene            complement(603..1658)
                     /locus_tag="VGQ51_17470"
     CDS             complement(603..1658)
                     /locus_tag="VGQ51_17470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9038432.1"
                     /translation="MKTIKQFRSDLRTRAGYGTAFILLLSCYLLTVYANSELLKEARV
                     IAYSERAVTPKEMIALTARHRFLDNVIFVSVSIAVLVAIFAWLTYSRENKARRIADQQ
                     LAANQQQLQERVQDLDKANKSLQQIRSVEKFAATGRIARTIAHEVRNPLTNINLSVEQ
                     LQAELEGGPTKNYRLLLEMISRNSNRINGLITDLLNSTKFTDLVYQRISINELIEETL
                     DLARDRIRLNNFDVTRDYMSDTCEVSVDVRRMKIALLNIIVNALEAMEPGQGILHLKT
                     AVRNNKCVITISDNGAGIDEDTLPKVFEPYYTSKASGTGLGLTNTQNIILNHNGSIML
                     DSKKGVGTSFIIQLELA"
     gene            complement(1667..3199)
                     /locus_tag="VGQ51_17475"
     CDS             complement(1667..3199)
                     /locus_tag="VGQ51_17475"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV9038433.1"
                     /translation="MGKGMWYHFWGWGGMQNGTILQYFHWYYPTDGSLWKKLAAEAPH
                     LAGMGFTAIWLPPASKGTNGTFSVGYDVYDPYDLGEFDQKGSVRTKYGTKQELMSAVE
                     AIHKAGMQVYLDVVQNHLAGGDQKETVRARKVDPEDRNKFISDPYDIEAFTRFTFPGR
                     KGKYSPFIWDHHCFTGVDYDSRTKEMAIYSIQNDYGEGWEDVIDEEKGNYDYLMYDDV
                     EFRNPHVREELKKWGEWLWNLTHFDGVRIDAVKHIPPGWENEWLDHMRKVTGSEFFAV
                     GEYWAPGNLPLLLKYIDATEGRMSLFDASLHHNLFEAGKKGQDFDLTTIFNETLVAVK
                     PELAVTVVTNHDTQPLQALEAPVDDWFKPLAYALLLLRDTGYPCVFYADLYGAGYKDK
                     GADGNEYRIDMQPVEQIDRLMTARKRFAYGTQRDYLDHPCCIGWTREGDDEHEDSGCA
                     VLICNGDEGFKTMEVGKRHAGRKFVDWLNKYPGTVTIDEEGKGEFHVGGRAVSVWVQE
                     GV"
     gene            3352..3591
                     /locus_tag="VGQ51_17480"
     CDS             3352..3591
                     /locus_tag="VGQ51_17480"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV9038434.1"
                     /translation="MSNNTSKVLLTFIAGAAIGAALGVLLAPDKGSETRRKLMEKGSD
                     IGDSIAGFGETVKEKFNDVVEGMKGSFTSQKNSMS"
     gene            3588..3935
                     /locus_tag="VGQ51_17485"
     CDS             3588..3935
                     /locus_tag="VGQ51_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038435.1"
                     /translation="MSMQEEPTDIDILLSDAGDFIETKATLWKLKTIESLADVSGELV
                     AGLAMIVFASFVVILISIGVALWLGEILGKSYYGFFIVGGFYAIIALIILVRRERWLK
                     DTFSNMLIRKILK"
     gene            3932..4198
                     /locus_tag="VGQ51_17490"
     CDS             3932..4198
                     /locus_tag="VGQ51_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038436.1"
                     /translation="MKTAKITTIGQLRETIRDLEHQDYVNQQHMRSRVGEVADRFKPA
                     NIFKRLIGNLFFDGHETKMGLLRVVAGFATSLIVKKFFKKGLRR"
     gene            complement(4290..4601)
                     /locus_tag="VGQ51_17495"
     CDS             complement(4290..4601)
                     /locus_tag="VGQ51_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038437.1"
                     /translation="MPDTKTEKFILFEQFIDRYYPSIFAGVQKLAGPVEKLVLEKLTV
                     EIFIDLWEHSDELFKPLRKPAFVYKVLVRHVIAWLKKTGAGEQLAFLQNTLLIDPDQE
                     G"
     gene            complement(4987..6387)
                     /locus_tag="VGQ51_17500"
     CDS             complement(4987..6387)
                     /locus_tag="VGQ51_17500"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEV9038438.1"
                     /translation="MRKLTALLAALSLTATAFCQQGNPRLANASREDKNGWIYVHLEG
                     SPGNIGYQHGYLLADEIDDLIKAMKVSLPHLSGKDWSFYRDAVKKMFWDKIDKEYQDE
                     IAGIVEGLRARGKHYDVYDLVVLNGNIELSQYYVPWLEGKQHPGSTAANNKAPGNCSG
                     FIATGSYTADGLIAIGHNNWTDYLGGERWNIIADIVPQTGNHIFMDCMPGLIHSGDDF
                     VVTSGGILITETTITQFKGFDEKGIPEFVRARKAAQYANSIDDFVQIMTTGNNGGYAN
                     DWLVGDIKTNEIARVELGLKHFRVWRTKDGVYTGSNFPLDSTLIATETTFNPADTTNS
                     PNSRRRRWEHLTAQYKGRLDVETAKTIEGDSYNELTNAREASRCVIAGRADTDPNGIP
                     EWSWPPFFPGGTVQAKITTTTLAKDLKFWAHMGNPDGEDFLAAPFLAAHPEYEWESRY
                     LRDMKAWPWTLFEAKR"
     gene            complement(6401..7675)
                     /locus_tag="VGQ51_17505"
     CDS             complement(6401..7675)
                     /locus_tag="VGQ51_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038439.1"
                     /translation="MNRLIKIAAPIVLFAFTFQRLNAQDSLSINKLNSAPAAHPPAAH
                     KYGLFSHDPNNQTDLVDLGRSFLKGHRSTRIDTIKQKVGKLHISALPVAGYTLQTGFA
                     GVLSSNFAFYTSEHSNMSNVLTSFTYSQYQQIIFPIQANIWTEGNRYNIVTDWRYLKY
                     PSLTYGLGGDTNADSGYNIDYGYIRLHQAVLRSVTKDLYLGIGYDFDYFWNVREIDPP
                     PGKPTDFERYGLSSSEQASGISLRAQFDNRRNPINPENGTYFNVNYRDNFTWLGSSDN
                     WQSLLFDAREYIRFPGSTHNVLAIWSYDWLTVGNGNPPYLLLPSTGWDAYSNTGRGYI
                     QGRFRSRNMLYFESEYRFGITSNGILGGVAFGNAQSFSDPVTGRFNTIAPGYGGGIRI
                     SLNKFSRTNLCIDYGWGIHGSGGFFVNLGEVF"
     gene            complement(7789..>8649)
                     /locus_tag="VGQ51_17510"
     CDS             complement(7789..>8649)
                     /locus_tag="VGQ51_17510"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEV9038440.1"
                     /translation="FNRFSIYHQLLNMPRWKFMALVFLIFFIVNVSYALIYYCIGSTE
                     LTGLVADTPWGKFKEAFFFSTETFTTVGYGRVNPVGDGANIVAAIEALTGLLSFAIFA
                     GLMFGRFSRPRSYLVFSDNALISPFRGGTGLMFRFAPYKDNHTLTDVQIRVNVGLKVQ
                     MDGNAEYRYYDLNLERTKVESLPMNWTVVHPIDEQSPFLGFTQEDMVAADVELYVLIR
                     GFDDVFSNLVQQRTSYTYQEIIFNGKFVPMYRESEDGKTTILELHKLNEFIPVNVPSV
                     TLPAPSPAKL"
BASE COUNT         1957 a         2269 c         2280 g         2143 t
ORIGIN      
        1 ttggcgtcaa tattgtgtat tataaacagg gcaaggcaaa attagagcca gggacgcccc
       61 ggaaatatcg tggcaataaa attgtaaata ttctgttatg ataaaattgt ggggattcta
      121 aatttatgtg ggtaagtgga aacaattatt atgacagaca ctaataataa agtccttatc
      181 gtagacgatg aactggatat ttgttatttg ctaagcggca tgttgaaaca gagaaatttc
      241 gtaacaggct tcgtgaatac gctttcagac gcagcgaatg cacttcagac ggatacgcct
      301 tccttgttat tccttgataa tcatctgccc gatggttttg ggttggattt cattccgtat
      361 atcaaaaaga attatcctga agtaaaggtg atcatgatca cggcgcacga cggcgccgcg
      421 gacaggaaac aggcctatga cggtggcgtc gatctttttg ttgccaaacc gctgaatcgc
      481 aagatgatca atgatgcgat cgacaaatta tatagtactg accccgcagg gcataaagcc
      541 tatcgttttc aatagcccat gcggcttgtg tagcgggtat tacccactct tccccatttc
      601 tctcacgcga gctcgagctg gatgatgaag gacgtaccga cgcccttttt actgtcgagc
      661 atgatcgagc cattgtggtt aaggatgata ttctgggtgt tggtgagccc cagaccggtg
      721 cccgaagctt tgctggtgta gtagggctcg aataccttcg gcaacgtatc ttcgtctata
      781 ccagccccgt tatcgcttat cgttatcaca catttattgt ttctgacagc cgtcttcagg
      841 tgcaggatgc cctgaccggg ctccatcgcc tccagcgcat tgacgatgat attgagcagc
      901 gcgatcttca ttcttcgcac atccaccgag acttcgcagg tatcggacat ataatctctc
      961 gtgacatcga agttattcag ccggatacga tcccgcgcca gatcgagcgt ttcttcgatc
     1021 aactcgttga tcgaaatgcg ctggtagacc aggtcggtga atttggtgga attcagcaga
     1081 tcggtgatca gcccgttgat gcggttgctg ttgcggctga tcatctccag tagcaggcgg
     1141 tagttcttcg taggcccacc ttccagctcg gcctgcagtt gctcgaccga gagattgata
     1201 ttggtcagcg gattacgcac ctcgtgcgcg atggttctcg ctatccggcc ggtagccgcg
     1261 aatttctcca cgctgcgtat ttgctgcaat gatttattgg ccttgtcgag atcctggacc
     1321 ctttcctgca attgttgctg atttgccgcc aattgttggt cggcaatgcg ccgggccttg
     1381 ttctcgcgcg agtaggtcag ccaggcgaag atagcgacga gaacggcaat actgaccgaa
     1441 acgaaaatga cattatccag aaagcggtgt cgcgccgtca gggcgatcat ctcctttggg
     1501 gtcacagcgc gttcggagta agcgatcacc ctggcctctt taagcaactc ggaattggcg
     1561 tatacggtaa gcagatagca ggacagcagc aggataaagg ctgtgccgta ccctgcgcgt
     1621 gttcgcaggt cggatctgaa ttgtttgatc gttttcatat tttccgtcac accccctcct
     1681 gcacccatac ggacaccgcc cggccgccga catggaactc ccctttgcct tcttcgtcga
     1741 tggtaacggt ccccgggtac ttgttcagcc aatcgacaaa cttcctgccg gcatgtcttt
     1801 tcccgacctc catggtcttg aatccttcgt ctccgttgca gatgagcacg gcgcaaccgg
     1861 aatcctcatg ctcgtcgtca ccctcccttg tccatccgat gcaacacgga tgatcgaggt
     1921 aatcccgctg ggtcccatag gcgaaccgct tgcgcgccgt catcagccgg tcgatctgct
     1981 ccaccggctg catatctatc cgatattcat tgccatcggc gcccttgtct ttatatccgg
     2041 caccatagag gtccgcataa aacacgcagg gatagcccgt atcgcgcaac aataacagag
     2101 cataggccag cggtttgaac cagtcatcta caggcgcttc cagggcttgc aggggctgcg
     2161 tgtcatgatt ggtgaccacc gtcaccgcca gctccggttt taccgccacc agcgtttcgt
     2221 tgaatatggt cgtcaggtcg aaatcctgcc cctttttgcc cgcctcgaac agattgtgat
     2281 gcagcgaagc gtcgaacagc gacattctcc cctctgtggc atcgatatac ttcagcagca
     2341 gcgggagatt gcccggcgcc caatattcgc ctaccgcaaa gaactcgctc ccggttactt
     2401 tacgcatatg atccagccat tcgttctccc aaccgggcgg gatatgcttt actgcgtcga
     2461 tccggacccc atcgaaatgc gtcaggttcc agagccactc cccccatttc ttcagttcct
     2521 cccttacgtg cgggttccgg aattcgacat cgtcatacat caggtagtcg taattgccct
     2581 tttcctcgtc gatcacgtcc tcccagccct cgccatagtc gttctggatg ctgtagatcg
     2641 ccatttcctt cgtccggctg tcataatcga cacctgtaaa gcagtgatga tcccagatga
     2701 aaggcgaata ttttcctttt cggcctggaa aggtaaaccg ggtaaaggcc tctatatcat
     2761 agggatcgct gatgaatttg ttccggtctt cggggtcgac cttgcgggca cggaccgtct
     2821 ccttttggtc gccgcccgcc aggtggttct gtacgacatc gagatagacc tgcatgccgg
     2881 ccttgtgtat ggcctccacc gcggacatca gctcttgttt ggtgccatat ttagtccgga
     2941 ccgatccttt ttgatcgaat tctccgagat catacggatc ataaacatca taaccgactg
     3001 agaatgtgcc attagttcct ttgctggcgg ggggtagcca aatggcggta aagcccatac
     3061 cggccagatg gggggcttcc gcggccaatt ttttccataa gctgccgtct gtaggataat
     3121 accagtgaaa atactgcagg atagtgccgt tttgcatacc tccccatccc cagaaatgat
     3181 accacattcc cttacccata aaaccaacac ccccttgctc agaatgtaga acttttttca
     3241 caaatacccc ctctctttgc gaaatactgt cctatagaag atttggcaag gattttttgg
     3301 gtactaagag aagagacgct aaacttgaag tcttactaaa atggagaaac tatgagtaac
     3361 aacacatcga aagtcctgtt gacctttatc gccggagcgg ccatcggcgc ggcgcttggc
     3421 gtattgcttg cgcccgataa aggatcggag acgcgccgta aattaatgga aaaaggatcc
     3481 gatatcgggg acagcatcgc cggtttcgga gagaccgtaa aagaaaaatt caatgatgtg
     3541 gtcgaaggga tgaaaggcag cttcaccagt caaaagaact ccatgtcgtg agcatgcaag
     3601 aagaacctac ggatatcgat attcttctgt ccgatgcggg cgatttcatc gagaccaaag
     3661 caacgctgtg gaagctgaag acgatcgaga gtcttgccga tgtttccggg gaactggtag
     3721 ccggtctggc tatgatcgtg ttcgcgtcgt tcgtggtgat cctgatcagc atcggggtgg
     3781 ccttatggct cggggagata ctgggcaaga gttactatgg gttttttatc gtcggcgggt
     3841 tctatgcgat catcgcactg atcatactgg tgcggcgcga gcggtggttg aaggacacct
     3901 ttagcaatat gctgatcaga aagatattaa aatgaagacg gctaagatca ccaccatcgg
     3961 gcagttgcgc gagacgatcc gcgatctgga gcaccaggat tatgttaacc agcaacacat
     4021 gcgtagccgg gtaggggagg ttgcggatcg gttcaagccg gccaatatct tcaagcgcct
     4081 gatcggcaat ctattcttcg acggccatga gacaaaaatg gggttgctca gggtcgtcgc
     4141 cggatttgcg accagtctta tcgtaaagaa attctttaaa aagggattgc gccggtaggg
     4201 ggaggatcct ctacgtctca gcgaggcttg ccgccgggac ttcgacagac ggccggggac
     4261 ttccgggttc gcgaaccgcc ctccgtggct cagccttcct gatcgggatc gatcagcagc
     4321 gtattctgta agaatgccag ctgttcgccg gcccccgtct ttttcagcca ggcgatgaca
     4381 tgcctgacca gcaccttata gacaaaagcg ggttttctga gtggtttgaa gagctcgtcg
     4441 ctatgctccc acaggtcgat aaagatctcg accgtcaact tctcgagcac cagcttctcg
     4501 accggtcccg cgagtttttg gacccctgcg aatatcgagg ggtaatagcg atcgatgaat
     4561 tgttcaaaca ggatgaactt ttcggtcttg gtatcaggca tggcgatcaa cattggtaag
     4621 gatgattaac catagtcacc gccaaacagg aggatgtaac cgctaaacag aagatttttc
     4681 catgatcggt atcaagttat cgtcaatttt aggcgcattt cgcctttatg gtatatattt
     4741 tgtgtcaaat tatagactta acctgcgatt tgcctgaatt ttcgaaagat caacttatac
     4801 ttcctgtgat caaccttaaa agagtacaca cgttctactg ctcggccttt aaggcattgg
     4861 cataagccgt tcacaaaggg gtaatgtatg gagggcccac cctacgttac ctttttttat
     4921 gggcataccc caaagcggcg gcactcgcgg ttgcgtcgac ctgcggaatg cgctgccccg
     4981 gcatccctac cgtttagcct cgaacagcgt ccacggccat gccttcatat cccgcaggta
     5041 tctcgattcc cattcatatt ccggatgggc cgccagaaag ggggcggcca gaaaatcctc
     5101 gccatcgggg ttacccatat gcgcccagaa cttcaggtct ttggccagtg tggtcgtagt
     5161 gatcttcgcc tgaaccgtac caccggggaa aaaaggaggc cagctccatt ccgggatgcc
     5221 gttcgggtcg gtatcggcac gaccggcgat gacgcagcgg ctggcttcgc gggcattggt
     5281 cagctcatta taagagtcgc cttctatcgt cttggccgtc tccacatcca gtcgcccctt
     5341 gtactgggcg gtgaggtgct cccaccgccg gcgccggcta ttgggggaat tggtggtgtc
     5401 ggccgggttg aaagtcgtct cggtggcgat gagtgtcgaa tccagcggga agttcgaccc
     5461 ggtgtagacg ccgtccttcg tccgccatac ccggaaatgt ttcagaccaa gctcgacccg
     5521 ggcgatctca ttggtcttga tatcgcctac cagccagtcg ttcgcataac ccccattgtt
     5581 acctgtcgtc atgatctgta cgaaatcgtc gatgctgttg gcatactgcg cggcttttcg
     5641 cgcccggacg aactccggta tccctttttc atcgaacccc ttgaattgcg tgatcgtggt
     5701 ctccgtgatg aggatgcccc cgctggtgac cacgaaatca tctccgctgt ggatcaaccc
     5761 cggcatacaa tccatgaata tatggttgcc cgtttgcggg acgatgtcgg caatgatatt
     5821 ccatctttct ccgcccagat agtcggtcca gttgttgtgc ccgatggcga tgagaccgtc
     5881 ggcggtatag ctgccggtgg cgatgaaccc gctgcagttg ccgggggcct tgttattggc
     5941 ggctgtcgat cccggatgtt gtttcccttc cagccagggc acataatatt gcgaaagctc
     6001 gatatttcca ttcaacacca cgaggtcata tacgtcatag tgtttcccgc gcgccctcag
     6061 tccttcgacg atgccggcga tctcgtcctg gtattccttg tcaatcttat cccagaacat
     6121 tttttttacg gcgtcgcgat agaaggacca gtcctttccg gagagatggg ggagcgagac
     6181 cttcatggct ttgatgagat cgtcgatctc gtcggccagc agatagccgt gctggtagcc
     6241 gatgttgccg gggctacctt cgagatggac atagatccag ccattcttgt cctcacgcga
     6301 ggcgttggcg agacgggggt tgccttgctg gcagaaagca gtggccgtca gcgacagggc
     6361 tgccagcagc gcggttaatt ttctcatata gggaagttac tcaaaaaact tcaccgagat
     6421 tcacaaaaaa accgcccgat ccgtggatgc cccagccata gtcgatacag agattggtgc
     6481 gcgagaactt gttcaaactg atccggatgc cgccgccata gccgggtgcg atcgtattga
     6541 atctgccggt gacggggtcg gaaaaagatt gggcgttgcc gaaagcgacc ccgccgagaa
     6601 tgccgttcga agtgatgccg aagcggtatt ccgattcgaa atagagcata ttcctgctgc
     6661 ggaaacggcc ctggatataa cccctgcccg tattgctata ggcatcccaa cccgtgctgg
     6721 gcaaaagcag gtaaggagga ttgccattcc cgacggtcag ccagtcgtag ctccatatcg
     6781 ccaggacatt atgcgtcgac ccggggaagc ggatatattc ccgggcatcg aataacagcg
     6841 attgccagtt gtcgctgctg cccagccagg taaaattgtc gcgatagttg acattgaaat
     6901 aggtgccgtt ctcgggattg atggggttgc gccggttgtc gaactgggcg cgcagggata
     6961 tccctgaagc ctgttcggaa ctggagagac cgtaccgttc gaaatcggtg ggtttgccgg
     7021 gtggcggatc gatctcccgg acattccaga aatagtcgaa atcatagccg atgcctagat
     7081 aaagatcctt cgtcacactt cgaagaacgg cctggtgcag ccttatataa ccataatcga
     7141 tgttgtaccc gctgtcggcg ttggtgtccc cgcccagccc ataggtcagc gaaggatatt
     7201 tcaaatatcg ccagtcggtg acgatattgt atcgattgcc ttcggtccag atattggcct
     7261 gtatcgggaa gatgatctgc tggtattgag agtaggtgaa gctcgtcagg acattcgaca
     7321 tattgctgtg ctcgctggta tagaacgcga aattcgagct cagcaccccc gcaaaaccgg
     7381 tctgaagggt atatcccgcc accggaagcg cagaaatatg caattttccc actttttgct
     7441 ttatcgtatc gatcctcgtc gatctatggc cctttagaaa actgcggccg aggtccacca
     7501 ggtccgtctg attgttgggg tcatgggaga acagcccgta tttgtgtgcg gccggcgggt
     7561 gtgcggccgg cgcactattt aatttgttga tagacaaaga gtcctgcgcg ttgagtcgct
     7621 ggaaggtgaa agcaaagagt acgatcggcg ctgctatttt aatgagtctg ttcaacaatg
     7681 tcggcttaat agtgatgttg gaccgttgga tactataacg gtttactgtt acgggctatt
     7741 tcgcccggtc atgatgtctt ttcaattgtc gggcgtaacc tggcggtgtc atagcttcgc
     7801 aggagagggg gcgggaagag ttacggaagg aacattcaca gggatgaact cgttcagttt
     7861 atggagctct aagatagtgg tttttccatc ttcgctttcc ctgtacatag gcacaaactt
     7921 accgttgaaa ataatttcct gataggtgta tgaggtacgt tgctggacca aattcgaaaa
     7981 gacatcgtcg aaaccgcgga tcagcacata cagctccaca tccgctgcca ccatatcttc
     8041 ctgcgtgaag cccaggaacg ggctttgttc gtcgatggga tggaccaccg tccagttcat
     8101 gggcaggctt tcgaccttgg tcctttcgag attcaggtca taataccggt attccgcatt
     8161 cccatccatc tggaccttaa ggcccacatt taccctgatc tgtacgtccg tcagcgtatg
     8221 gttatcctta tagggggcga accggaacat gagccccgtc ccgcccctga acgggctgat
     8281 cagcgcatta tcgctaaaca ccaggtagct ccggggccgt gaaaaacgcc cgaacatgag
     8341 acccgcgaag atggcaaaag acagcagacc tgtcagcgcc tcgatggccg cgacgatatt
     8401 cgctccgtcg cccaccgggt tcacccgacc ataacccacc gtagtaaagg tctcggtgct
     8461 gaagaaaaag gcctccttga attttcccca gggcgtgtcg gcgaccagcc cagtcagctc
     8521 tgtgctgccg atgcaataat agatcagggc ataactcacg ttgacgataa aaaagatgag
     8581 aaataccaat gccatgaatt tccatcgcgg catattgagc aactggtgat agatgctgaa
     8641 gcggttgaa
//
LOCUS       DASYSV010000561         5433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1646654, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000561 DASYSV010000000
VERSION     DASYSV010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646654"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VGQ51_17515"
     CDS             <1..828
                     /locus_tag="VGQ51_17515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV9038441.1"
                     /translation="PGGAIGEGNIRGLAWLAAEGYDIYLYDQVGGGFSNRLRNVRDYT
                     TERHVRDLDAIVQQVGAKKVILLGHSWGAILAVLYAADHPDRVDRLIVTGPGPIFPIR
                     SGLATERAPDSLHLKPPFYSNRQGNDQANNIRTHAMEFFATQFSWKIASDREADDFAG
                     YLNSLLERSTVADTSKIGPIRPIPGVGYYAGVMTYYSRLNTPDPRPRLRNTTIPLLVM
                     KGQYDNQKWGFTHEYLELFPVHRLVVVPNAGHSIGREQPALLDSTIRDFLKTTYIRQ"
     gene            838..1179
                     /locus_tag="VGQ51_17520"
     CDS             838..1179
                     /locus_tag="VGQ51_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV9038442.1"
                     /translation="MTYSSELLKGTLKTIVLKLLADNKKMYGYEITQRVKELTQDRIQ
                     ITEGALYPTLHSLEADGLVKTETEYIGKRMRKYYSLSPAGKTTSREKVNELASFMQTM
                     TLLLDLQPKLR"
     gene            1180..1731
                     /locus_tag="VGQ51_17525"
     CDS             1180..1731
                     /locus_tag="VGQ51_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038443.1"
                     /translation="MFAITDTHIDHILNDLSAKGLRIDDLRNNLLDHICILAERDLSE
                     GDDFEAWYRGVIPTFYRQQLTELEEETEFLLKHRACFAVLNRLQFFLLLFVFLVGPIL
                     CWIISTFGEEIITRTAAVEAWEAGLVFMVFPLLFLLVLFMTPRRFDPLIPPGAKILIG
                     WKPFVSVIPGDVMQEMICSHESS"
     gene            complement(1716..>5433)
                     /locus_tag="VGQ51_17530"
     CDS             complement(1716..>5433)
                     /locus_tag="VGQ51_17530"
                     /inference="COORDINATES: protein motif:HMM:NF036757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038444.1"
                     /translation="LRSAPDHKLIYSPQFTDFEKSLSYPFFYRDDSRAYFIRTTDISY
                     LSVQHLRQPSKTKFIPLQAVQYAEAPSYIPQPVGDPEAILNQAGLAVRAAANGRVAGP
                     AVNGQLPAMFEAMDTNALINSSPKAYAMSAKSSTIAGSFAGKQASPVYRVPFVFDKGL
                     TFYTNYHPYSQAFIQALNTKDIGGLMATNTLLPDDGGSLFEKVYSPDHTNGLVQLPAD
                     LAKFTYYKENVAFDEFSLYGMYNWELFYHAPLYIATRLSRNGMYQDAMKWFHYIFDPT
                     TNELSPDPNFPESRYWKVLPFKTTLPQSLLDYFESLQPGVEDPHIEEWKDNPFKPFLV
                     ARDRPIAFMKNVVMKYLDNLIAWGDDLFRTDTRENINTATQLYIIASHILGPKPQMVP
                     SRGIIKAETFDSLRAKLDDFSNAMVDLENLFPYTSDITVNDDAFPGSLLGVGSTLYFC
                     IPDNDRLLQYWDTVGDRLFKIRHCMNIDGVERSLALFAPPIDPGMLIRATAAGLSIGS
                     ILADLNSPAPIYRFNYLVQKATEFCAEVKALGSSLLMAIEKKENEELSRLRAAHETSI
                     LNKMTAVKERQILEAKANKESLGKSRDMIVNRINYYIDYLGITGFTMPAAPADLPDTI
                     DQDSTLPDTAITEVNLGLDTALADSGITGVKLIQQEQEQLDQSESALNSQLDSINYEI
                     LSGFMALIPDFNTHATPLGVGLALTFGGHALSWMASSEAKQKTGDSASASHSATKASI
                     MAGYIRRDQESVHQLNLALRELAQVDKQLVSSDIRVQLAKKELDTHQQQITNAQEIED
                     YLQNQLQGHTKFTTQEFYQWMKEQLFAVYKQSYQMAFDMARRAEKAYQFELGVTDSRF
                     IQYGYFDSSFQGLTAGEQLYSGIKQLEKSFTEQNKREFELTKHISLALTRPDLLLQLK
                     QTGSCEWTWPEELFDLDYPGHYFRRIKSVSISIPCVTGPYTTVNATLRLLSSSIRVRT
                     TRGDNGYIHNNDDGVLTDDDRFVDSNTPFVAIATSSAQNDSGVFELNFRDERYLPFEG
                     AGVISKWRLELNGKYLKQDNSVIDISQFDFNSVSDILAHVRFTSREDAGLFRLDALSH
                     LQDYFANAMANATAPLMRLFSARNDLPTQWYQFLHPVNTTDDQVLTLDLSPLRFPFFV
                     QKKKIGITGINLVADSTLADINGLQLVFPDNTTDTIGLTADGKYGNWLHGSKDYSQNK
                     KSPGVWNMKNPVTNARISEDQLKNLVIVVYYELS"
BASE COUNT         1235 a         1391 c         1497 g         1310 t
ORIGIN      
        1 ccgggcggag ccatcgggga agggaatatc cggggtctgg cgtggctggc ggcggaaggc
       61 tatgacattt acctgtatga ccaggtgggg ggcggctttt cgaaccggct gcggaatgtc
      121 cgggattata ccactgaacg acacgtacgc gacctggacg ccatcgtgca acaggtcggg
      181 gcgaaaaagg tgatccttct cggccattcc tggggagcga tacttgcggt gctttatgcc
      241 gccgatcatc ctgaccgtgt agaccggctc attgtgacgg gtcctggccc tatcttcccg
      301 atccggtccg gtcttgcaac cgaacgcgcg ccggacagcc tgcatttaaa gccgccattt
      361 tacagcaacc ggcaggggaa cgatcaggcc aataatatcc ggacccacgc catggagttc
      421 ttcgccacgc aattcagttg gaagatcgca tcggacaggg aggcggacga ttttgccggc
      481 tatctgaact cgttgctgga gaggtcgacg gtggccgaca cctcgaagat agggccgatc
      541 cggccgatac ccggcgtggg ttactatgcg ggggtaatga cttattacag ccggttgaat
      601 acccccgatc cgcgaccacg actccggaat accaccattc cgttactggt gatgaagggg
      661 cagtatgaca accagaaatg gggcttcacg catgaatacc tggagctttt tccggtgcac
      721 cggctggtcg tcgtccccaa tgccggacac agtatcggcc gcgaacagcc agccttgctc
      781 gactcgacga tcagggattt tttaaaaacc acgtatattc gtcaatgaaa tgcgcctatg
      841 acctactctt ccgaattact gaaagggaca ttaaagacca tagttctcaa gctgcttgcc
      901 gataacaaaa agatgtacgg ttatgagatc actcaacggg tgaaagagct gacccaggac
      961 aggatccaga tcaccgaggg agccttgtat ccgaccctgc attcgctgga agcggatgga
     1021 ctggtcaaaa ccgagaccga gtatatcggc aaacgtatgc ggaaatatta tagtcttagt
     1081 ccggcgggta agacgacttc aagagaaaag gtgaatgagc tcgcctcctt tatgcaaacc
     1141 atgacgcttt tgctcgatct gcaacccaaa ctcagataaa tgttcgccat caccgacacg
     1201 catatcgatc atattctcaa cgacctttcc gcgaaaggtc tgcggatcga cgatctgcgg
     1261 aacaatctgc tggatcatat ctgtatcctt gcggaacgcg atctcagcga gggagatgat
     1321 ttcgaagcgt ggtaccgggg tgtgatcccc accttttacc ggcaacaact aaccgagctg
     1381 gaagaggaga ccgagttttt gctgaagcac cgggcatgtt tcgcggtatt gaaccgtctg
     1441 cagtttttcc tgttgctatt cgtgtttctg gtcggaccga tcctctgctg gatcatttcc
     1501 acattcgggg aagagatcat tacacgaacg gcggccgtcg aggcctggga agcggggctg
     1561 gtattcatgg tttttccgct gctgttcctg ttggttctat tcatgacacc ccgccggttc
     1621 gaccccctga tccctccggg tgcgaagatc ctgataggct ggaagccgtt cgtttcggtc
     1681 attccggggg acgttatgca ggagatgatc tgcagtcacg aaagttcata atacaccacg
     1741 ataaccaggt tcttcagctg atcttcggaa atccgggcgt tggtcacggg gttcttcata
     1801 ttccagaccc ccggtgactt tttattctga gaataatctt ttgatccatg cagccaattc
     1861 ccatactttc catctgccgt gagcccgatg gtgtcggtgg tattgtcggg gaacaccagt
     1921 tgaagaccat ttatgtcggc taaagtgcta tcggcgacca ggtttatccc ggtgatgccg
     1981 atctttttct tttgtacgaa gaacgggaag cggagcgggc tgaggtctag cgtgagcacc
     2041 tggtcatctg tcgtattcac cggatgcagg aactgatacc actgcgtcgg cagatcgttc
     2101 ctggcgctga acagccgcat cagcggcgcg gtggcattgg ccatcgcatt ggcaaagtag
     2161 tcctgcaaat gactgagggc atccaacctg aaaagccctg catcctccct ggatgtaaag
     2221 cgaacatggg cgagaatatc cgaaacggaa ttgaagtcga actgggagat atcgatcacg
     2281 gagttgtcct gtttcaggta tttcccgttc aattcgagac gccatttgct gatgacgccc
     2341 gctccctcga aaggcagata ccgctcgtcc cggaagttga gctcgaaaac gccgctatcg
     2401 ttttgcgccg agctggtagc aatggccaca aaaggtgtat tgctatccac aaagcgatcg
     2461 tcatcggtca gaactccgtc gtcattgttg tggatatacc cattgtctcc gcgcgtcgtc
     2521 ctgacgcgaa tggaactact gagcaaacgc agggtcgcat taacggttgt ataggggccc
     2581 gtcacacagg ggatcgaaat actgaccgat ttgatccgcc ggaaataatg tcccggataa
     2641 tccagatcga acagctcttc cggccatgtc cattcgcagg aaccggtctg cttgagctgt
     2701 aaaagcaggt cgggccgggt aagggccagg gagatatgct tggtcagttc gaattcccgt
     2761 ttgttctgtt ctgtaaagga tttttccagc tgttttatac cgctatacag ctgctcaccg
     2821 gctgtcagtc cctggaagct gctatcgaaa tagccgtatt ggataaatcg ggaatccgta
     2881 acgcccagtt cgaattgata agctttttcc gctctccggg ccatatcgaa tgccatttga
     2941 taactctgtt tgtaaacggc aaacaactgt tctttcatcc attgatagaa ttcctgggtg
     3001 gtgaatttgg tgtggccctg aagctggttt tgcaaatagt cttctatttc ctgcgcattg
     3061 gtgatctgtt gttgatgagt atccagctct tttttcgcaa gctggacgcg gatgtcggaa
     3121 gaaaccagtt gtttgtccac ttgtgccagc tcgcgcaagg ccaggttgag ctggtgcacg
     3181 gactcctggt cgcgccggat atagccggcc atgatcgaag ctttggtcgc cgaatggctg
     3241 gcgctcgcgc tgtcgccggt cttttgtttt gcctcggaag aagccatcca gctaagggcg
     3301 tggccgccaa aagtgagcgc caatccaacg cccagcggag tagcatgcgt attgaaatcg
     3361 gggatcagag ccatgaaccc cgacaggatc tcgtagttga tggaatcgag ctgggaattc
     3421 aatgccgatt cgctttggtc caattgctcc tgctcttgct gaatgagctt tacgccggtg
     3481 atacccgagt cggccaaagc ggtgtcaagg ccaagattga cctctgttat cgccgtatca
     3541 ggcaaggtag aatcctggtc gatcgtatcg ggcagatcgg cgggagcggc gggcatggta
     3601 aaaccggtga tcccgagata atcgatataa taattgatcc ggttgacgat catatcccgg
     3661 ctttttccca gactttcctt attcgccttc gcctccagta tctgccgctc tttgacagcc
     3721 gtcatcttat tgagtatcga agtttcatgc gcggcccgga gcctggaaag ctcttcgttc
     3781 tcctttttct ctatcgccat caacagtgaa ctgcccagcg ccttcacctc ggcacagaac
     3841 tcggttgctt tctgtacaag ataattgaac cgatagatgg gagcggggct gttcagatcg
     3901 gcaaggatgc ttccgatact gaggccggcc gcggtcgccc ggatcagcat ccccggatcg
     3961 atggggggag cgaacagcgc gagtgacctt tccactccat cgatattcat gcaatgcctg
     4021 atcttgaaga gcctgtcgcc tacagtatcc cagtactgca acaggcggtc attatcggga
     4081 atgcagaaat acagggtgct tcccaccccg agtaagctgc cggggaacgc atcatcgttg
     4141 accgttatat cgctggtata tgggaacaga ttttccagat cgaccatggc attggaaaaa
     4201 tcatccagct ttgccctcag cgaatcgaat gtctcggcct ttattatccc ccgggacggg
     4261 accatctgtg gcttgggacc caggatatgc gaggcgatga tatacaactg ggtagccgta
     4321 ttgatatttt cgcgggtatc cgttcggaac agatcgtctc cccaggcgat gagattatcg
     4381 agatacttca tcaccacgtt cttcataaaa gcgataggcc tgtcgcgggc gacaaggaac
     4441 ggtttgaacg ggttgtcctt ccattcctcg atgtgcggat cttccacccc tggttgcaag
     4501 ctctcaaaat agtcgaggag gctctgcggc agcgtcgttt tgaaaggcag caccttccaa
     4561 taacggcttt cggggaaatt gggatcggga gacagctcat tcgtagtagg atcaaagata
     4621 taatggaacc acttcatcgc atcctggtac atcccattcc tgctcaaccg ggtcgcaatg
     4681 tacaggggtg catgatagaa gagctcccag ttatacatcc catatagcga gaactcatcg
     4741 aaggcgacgt tctccttata ataagtgaac ttggcaagat cggcaggcag ctgcaccagg
     4801 ccattggtgt gatcgggtga gtataccttt tcaaaaaggc tgcctccgtc atctggaaga
     4861 agggtattgg ttgccatgag ccccccgatg tcttttgtgt tcaatgcctg gatgaaggcc
     4921 tgcgagtagg ggtgatagtt ggtatagaag gtcagtccct tatcgaaaac gaacggcacc
     4981 ctataaacag gagacgcttg tttgccggca aaggacccgg caatggtgct gctttttgcg
     5041 ctcatggcgt aggccttcgg ggaactattt atcaaagcgt ttgtatccat ggcttcgaac
     5101 atggcgggga gctggccatt gacggccggc ccggcaaccc ggccattcgc agctgcccgg
     5161 acggcaaggc ctgcctggtt aaggatcgct tccgggtcgc caacaggctg cgggatataa
     5221 ctcggcgctt ccgcatattg cactgcctgc agagggatga acttcgtctt tgacggttgg
     5281 cgcaaatgct gaacactcag gtaggagata tcggtggttc gtatgaaata ggccctcgag
     5341 tcatcccggt aaaagaaggg atagctcaaa gatttctcaa aatcggtgaa ttgaggggaa
     5401 tagatgagct tatgatcggg ggccgatcgc aga
//
LOCUS       DASYSV010000562         6944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_497240, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000562 DASYSV010000000
VERSION     DASYSV010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497240"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="VGQ51_17535"
     CDS             <1..1547
                     /locus_tag="VGQ51_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HEV9038445.1"
                     /translation="FCAFLLSQSYAQRTPGQPRGESENTPAARPAERSSERPVPGSRT
                     LNLDSSVTTQAEVTIKGQRVPYTATAGTIPVWDDDGKPIAGVFYTYYERSDVNTKDRD
                     ARPLVISFNGGPGTPSVWMEIGYTGPRRLKLDDEGYPIEPYGVTENSQSILDVADIVY
                     IDPVNTGFSRPANKDVQATRFYGVNEDIKYLAEWISTFVSRKNRWASPKFLIGESYGT
                     TRVSGLALELQNNQWMYLDGVVLVSPTDLGIVRSDYVRMSLLVPYYAATAWYHKALAP
                     DLQNKDLTAYLPEVEDFTINELIPALARGGFIDSAKRISLAAKLARYIGLSEKAVLQN
                     NLEIPTQFFWKELLRDKGYTVGRLDSRYKGIDREEAGDRPDYNAELTSWLHEFTPAIN
                     IYLREDLRYKTDLRYLMFGPTFPWDNQNNHVGENLRQAIAMNPNLHLLIQSGYYDGAC
                     DFFNAEYSLWQLDPSGRLKDRLHWEGYRSGHMLYLRKPDLAEANENLRKFIRESMPKP
                     GQPAKY"
     gene            1554..2036
                     /locus_tag="VGQ51_17540"
     CDS             1554..2036
                     /locus_tag="VGQ51_17540"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9038446.1"
                     /translation="MRKILKETCPAAFLVKPYHISNVLVAVELALEGFAKQQPNTCDA
                     SRAAGTDREQLLLWKDQLFVKLNNRFVKIGLSSILYLAAESNYVHLITTEGKYPLRIG
                     LNQFMEKTVGSRLVRIHRSYAINVDAIQSFTEQEVRIGKEVLPIGRSYKEDFMQQFGG
                     "
     gene            complement(2057..5218)
                     /locus_tag="VGQ51_17545"
     CDS             complement(2057..5218)
                     /locus_tag="VGQ51_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038447.1"
                     /translation="MRKLLLSGCALFFSITVISQDLNSYLKPLKWRNIGPFRGGRSVT
                     SCGVVGDPMVYYMGTTGGGVWKTENAGWSWVNVSDGYFRTGSVGAVAVAESDPNVVYV
                     GMGEHAPRGVMSSYGDGVYKSTDAGKTWKKMGLDLTRHISAIRIHPSNPDIVYVAAQG
                     AVYGATEDRGVYESVDGGVTWKKVLYVDEHTGCADLSMDLHNPRILYAAIWDYQRLPW
                     EIRSGGKGSGLYKSTDGGATWTRMEKGLPKEMGKMGISVSGANPDKVYALIESNSQKE
                     EGGLFVSDNGGASWNRVSKDHRLVQRAWYYLEVFADPQNENIVYVLNSPGLKSIDGGR
                     TWSDLHGTHGDFHQLWINPRNDRNMIISNDGGAAVTFDDGDTWSTQNNQPTAQFYRIS
                     VDNLFPYRLYAGQQDNTSVMITSRNTRGGSIGEKDWGYSAGGESAFLAFDPDSPRYVM
                     GGSYQGTVEVLDMVSGEGKEVMISPIQYQSLDPKDMKYRFNWNAPIIYSRWERGAFYH
                     GGNRLFKTTDMGRNWEVVSPDLTRHDTAHMGTSGVPYTNEGAGGENYCTLAYVLESPL
                     EKGVIWTGSDDGVVSLTRDGGKTWSNVTPAGLAECLVNCIEVSPFDKATAYIATTRYK
                     FNDLAPGLYKTSDYGRTWKKIDNGIPMGAYTRAIREDDVRPGLLYAGTETGLYMSTDG
                     GAHWQQLQLNLPVTPITDLKIHQGNMIAATMGRGFWILDDLSALRQMVMGGSRKDLVL
                     YTPGDAYRVSGNSTLDKVVKDAGLERPRPALAGTNPATGVVMYYQLPEGRDSGKKLTL
                     EILDEGGHLVRRYSTKGDSTLVSFEGGPSPDVTMTDRVGLNRMVWDMRYPTVPGVPTV
                     FIEGSYEGRKAAPGVYRARLTYDTSVRTATFRVLADPRIAATAADYAEQQEWQARVED
                     GIRDIHGSVLRLRKVRRQISEVAELLGSRQDMKEVVDSGRKINARLLKWEEELVQNKA
                     QSNDDIINFINKLSADYIFLKGEMDANVPYVTQGQKTRYAELDAQWKVLRAQMNGLLE
                     KDVKGFNALCREKALEKVIVPEE"
     gene            complement(5248..6666)
                     /locus_tag="VGQ51_17550"
     CDS             complement(5248..6666)
                     /locus_tag="VGQ51_17550"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEV9038448.1"
                     /translation="MKKLTLLILGLALVPATWAQSWSLTGNAGTNPSVNFIGTTDNQS
                     LQFKVNNTNSGQLGVTGMTAFGYGAAQNGSGTISTAIGYRSLFSNPGYENVAVGGYTL
                     YYNNGGAYNTAVGFGAGELNTTGSYNTSMGYGAMVLTNTGHDNTAMGYSAMRGNTYGA
                     YSTALGDDALFGTTGSQYNTAVGYNAGSSFDLGYNNVLLGANCQGSFSGQYNMVAVGQ
                     GVVCPDNSTARIGNSATWSIGGWAGWSNFSDGRYKKDVKEDVKGLEFILKLRPITYRL
                     DVTGLSARLGENSGKEWDRQMKAAIADKEKMVFTGFVAQEVEKAADSLGFQFSGVDKP
                     KTENGLYALRYADFVVPLVKAIQEQQAMIQTLQAQVQALQQQADVAIVLQENGGERVS
                     TYPDPVVSAMTVSVHTQNAGAGILSVYDAGGQLVRQMNVSLQEGLNKFSLSMPMVASG
                     YYTLKIDWGNNNHKSVGFVKAN"
BASE COUNT         1578 a         2358 c         1487 g         1521 t
ORIGIN      
        1 ccttctgcgc gttcctcctt agccaaagct acgcccaaag aacccccggc caaccgaggg
       61 gcgaatccga gaacacacct gccgcaagac cagccgaacg ctcctccgaa cgtcccgtcc
      121 ccggcagccg cacgctcaac ctggattcct ccgtgacgac gcaggccgaa gtcactatca
      181 agggccaacg cgttccttac acggctacag ccggcaccat tcccgtctgg gacgatgacg
      241 gcaaaccgat cgctggcgta ttctacactt actatgaacg ttccgacgta aatacaaaag
      301 accgcgatgc ccgccccctc gtcatctcct tcaacggcgg ccccgggacc ccttccgtat
      361 ggatggagat aggctacaca ggtccccgca gactcaaact ggacgatgaa ggatatccca
      421 tcgagcctta tggcgtgacg gagaactcgc aatccatttt ggacgtggct gacatcgtct
      481 atatcgaccc ggtaaacacc ggcttctcgc gccccgcaaa caaagacgtc caggctacca
      541 ggttctatgg cgtcaacgag gatattaaat accttgccga atggatctcc acgttcgtgt
      601 cgcgtaagaa tcgctgggcg tcccccaagt tcctcattgg agagagctac ggaaccaccc
      661 gcgtatccgg tcttgccctc gagctgcaga acaaccaatg gatgtacctc gacggcgtcg
      721 tcctcgtatc ccccaccgac cttggcatcg tccgcagcga ctatgtaaga atgtcgctgc
      781 tcgtgcccta ctatgccgca accgcctggt atcacaaggc cctcgccccc gatctccaaa
      841 acaaggacct cacggcctat ttgcccgaag tggaagattt tacgatcaac gagctgatcc
      901 cggccctcgc ccgaggcggt ttcatcgata gcgccaaacg catttccctc gcggctaagc
      961 ttgcacgtta tataggcctc agcgaaaaag ccgtactcca aaacaatctt gagataccta
     1021 cccaattctt ctggaaagag ctattgagag acaaaggcta taccgtcggc cggctcgact
     1081 cgcgctataa gggcatcgac cgcgaagaag caggcgaccg ccccgactac aatgccgaac
     1141 tcacctcctg gctgcatgaa ttcacacccg cgatcaatat ctatttgcgc gaagacctcc
     1201 gttataagac ggatctccgc tatctgatgt tcggccccac tttcccctgg gacaaccaga
     1261 acaaccatgt cggtgaaaat ctccgtcagg ccatcgccat gaaccccaac ctgcacctgc
     1321 tcatccaatc cggctactat gacggcgcct gcgatttctt caatgccgaa tacagccttt
     1381 ggcaactgga ccccagcggc agactgaaag accgccttca ctgggaaggg tatcgcagcg
     1441 gacacatgct ctacctgcgc aaacccgatc ttgccgaagc gaacgagaat ctcagaaagt
     1501 tcatccgcga atccatgccc aaacccggcc agccggcgaa atactaacga cccatgcgca
     1561 agatcctgaa ggagacttgt ccggcggcct tcctggtaaa gccgtatcat atcagcaatg
     1621 tactcgtcgc ggtggaactg gcattggagg gatttgcgaa acagcagccg aatacctgcg
     1681 atgcaagccg cgctgccggg acggatagag agcagctact gctctggaaa gatcagcttt
     1741 tcgtgaaatt aaacaaccgg ttcgtaaaga tcggactgtc ctccatccta tacctcgccg
     1801 ccgaaagtaa ttatgttcat ctcatcacca cagagggaaa gtacccctta cgcataggcc
     1861 tcaaccaatt catggagaag acggttggaa gcaggctcgt ccgtatccac cggtcctatg
     1921 cgatcaatgt ggatgcgata caatctttta ccgagcagga agtgaggatc ggcaaagagg
     1981 tcctgccgat cggccggtcc tacaaggagg atttcatgca gcaattcgga ggttgacacc
     2041 cgcgcccgcc cccgccctat tcctccggca cgatcacctt ctccaacgcc ttctcccgac
     2101 acaacgcatt gaatcccttc acatccttct ccaacaaccc attcatctgc gcccgcaaca
     2161 ccttccattg cgcatccagc tccgcatacc gcgtcttctg cccctgcgtc acatacggta
     2221 cattcgcatc catctcccct ttcagaaaaa tatagtccgc actcagcttg ttgatgaaat
     2281 tgatgatatc gtcattgctc tgcgccttgt tctgcaccag ctcctcctcc catttcaaca
     2341 acctcgcatt gatcttcctg ccgctgtcca ccacttcctt catatcctgc ctcgaaccca
     2401 acagctccgc cacctcactg atctgccggc gcaccttccg caacctcaac acagatccat
     2461 gtatgtccct gatcccgtct tccacacgag cctgccactc ctgttgctcc gcataatccg
     2521 cagccgtggc tgcgatccgc ggatcggcaa gaacccgaaa tgtggccgtc cggacggaag
     2581 tatcataagt caacctcgcc cgataaacac ccggcgcagc cttcctccct tcataacttc
     2641 cctcgatgaa caccgttggc actcccggca ccgtgggata ccgcatatcc caaaccatac
     2701 gattcagtcc cacccgatcc gtcatcgtca catccggcga cggccccccc tcaaaagaca
     2761 ccagcgtcga atccccttta gtactatacc gccgcacgag atgcccaccc tcatccaata
     2821 tctccaacgt cagcttctta cccgaatccc gcccctccgg caactgataa tacattacca
     2881 ctcccgtcgc aggattcgtt cccgccaacg cgggccgcgg cctctccagc cccgcgtcct
     2941 tcacgacctt gtccaatgtg ctattccccg acacccggta agcatcaccc ggagtatata
     3001 acacgaggtc ctttcgcgat ccgcccatga ccatctgccg caaggcagac agatcatcca
     3061 atatccaaaa cccgcgaccc atcgtagccg ctatcatatt cccctgatgg atcttaagat
     3121 ccgtgatcgg cgtaaccggc aggttcaact gcaactgctg ccaatgcgcg cctccatccg
     3181 tcgacatata aagccccgtc tccgtccccg catacaaaag cccgggccgc acatcatcct
     3241 cacggatcgc ccgggtgtag gcgcccatag gtatcccatt atcgatcttc ttccacgttc
     3301 gcccatagtc cgaagtctta taaagccccg gcgccagatc attgaactta tatctcgtag
     3361 tggcaatata tgccgtcgcc ttgtcgaaag gcgacacttc gatacagttc accagacact
     3421 cggccaaccc cgcaggcgtc acattgctcc aggtcttgcc gccatcccgt gtcagactca
     3481 ctacgccatc atcgctcccc gtccagatca cgcctttctc caaaggcgat tccaacacat
     3541 aagccagcgt acaataattt tccccacctg ccccttcatt ggtataaggt accccactcg
     3601 ttcccatatg cgccgtatca tgacgggtaa gatcaggcga taccacctcc cagttgcgac
     3661 ccatatccgt cgttttaaaa agacgattcc ccccgtgata aaaggcaccc cgctcccacc
     3721 gggaatagat gatcggcgca ttccagttga acctgtattt catatccttc gggtccagac
     3781 tctggtactg tatcggggag atcatgacct ccttgccttc cccgctgacc atgtccagca
     3841 cttcgacggt cccctgatag ctcccgccca tcacataacg cggactgtcg ggatcgaagg
     3901 cgagaaaagc actttcgcca cccgccgaat acccccagtc tttttccccg atggatcctc
     3961 cgcgtgtatt acggctggtg atcatcaccg aggtgttgtc ctgctgccct gcataaagcc
     4021 tgtatggaaa aagattgtcc acactgatcc gatagaattg cgccgtcggc tgattgttct
     4081 gcgtgctcca ggtgtcacca tcgtcgaagg tgactgcagc ccctccgtca ttgctgatga
     4141 tcatgttccg gtcgttacgg ggattgatcc acagctgatg aaagtcgcca tgcgtcccgt
     4201 gcaaatcact ccacgtcctg cccccatcga tactcttcag acccggcgag ttcaaaacat
     4261 agacgatatt ctcattttga gggtcggcga acacttcgag ataataccag gcgcgctgca
     4321 ccagccggtg atccttgctg acacggttcc agctcgcacc gccattatcc gacacgaaca
     4381 acccgccttc ctccttctgc gagttacttt cgatcagggc atacaccttg tcgggattcg
     4441 cacccgatac cgagatgccc atcttaccca tttccttcgg caaccccttt tccatccgcg
     4501 tccacgtagc gccgccgtcc gtactcttat acaacccgct gccttttcca ccgctgcgta
     4561 tctcccacgg aagacgctga taatcccata tcgctgcata cagtatccgg ggattatgca
     4621 gatccatgct cagatcggcg catcccgtat gctcatcgac gtagagcacc ttcttccacg
     4681 tcaccccgcc atcgacactt tcatacacgc cccggtcctc agtagcccca tacacagcgc
     4741 cctgcgccgc gacatatacg atatcaggat tcgaaggatg gatacggatc gccgagatat
     4801 gccgcgtcag atccaatccc attttcttcc aagtcttccc cgcatccgtc gatttataga
     4861 ccccatcgcc atagctgctc atcacccccc gcggcgcgtg ctcacccatc ccgacataga
     4921 ccacgttcgg atcactctcc gctacggcca cagctcccac cgaccccgtc ctgaaatatc
     4981 catccgaaac atttacccac gaccaacccg cattctccgt tttccaaacg cctccacccg
     5041 tggtgcccat ataatacacc atcgggtcac ctacgacacc gcacgacgtg accgatcgcc
     5101 cgccgcgaaa cggtccgata ttccgccatt taagaggttt aagatagctg ttgagatcct
     5161 gcgaaatgac tgttatggag aaaaagaggg cgcagcccga gagcagcagc tttctcatgt
     5221 ttgatggagg tttagtatcg gaagatacta attcgccttc acaaacccca cactcttatg
     5281 attattattg ccccaatcga tcttcaatgt atagtacccg gacgcgacca ttggcatcga
     5341 caggctgaac ttattcaaac cttcctgaag actcacattc atctgccgca ccaactgacc
     5401 tccggcatca tacacgctca atatccccgc gcccgcattc tgggtatgca cgctcaccgt
     5461 cattgcactg actaccgggt ccggataagt gctgaccctc tcacccccat tctcctgcag
     5521 cacgatcgct acgtctgcct gttgctgcaa ggcctgcacc tgcgcctgca acgtctggat
     5581 catcgcctgc tgctcctgta tggccttcac gagcggcacc acgaaatctg catagcgtaa
     5641 cgcatataac ccattctccg tcttcggctt gtcgactccg ctgaattgga aacccaacga
     5701 atcggcagcc ttctctacct cctgcgcaac gaatcccgtg aataccattt tctctttatc
     5761 ggcaatcgcg gctttcatct gccgatccca ttcctttcca ctattctctc caagccgggc
     5821 gctgagcccg gttacatcca gccggtaggt gatcggtctc aacttgagga tgaactccaa
     5881 ccctttcaca tcctctttga catccttctt gtatcgcccg tcgctgaaat tactccaccc
     5941 ggcccaaccg ccgatggacc atgtcgcgga attgccgatc ctcgccgtgc tgttatccgg
     6001 acagaccacc ccctgtccga cggcgaccat attgtattgt cccgaaaaac tgccctggca
     6061 gttggcgccc aataggacgt tgttataccc caggtcgaag ctcgagcccg cattatagcc
     6121 taccgcggta ttatattgcg atcctgtcgt accaaataac gcatcgtccc caagggctgt
     6181 gctatacgcg ccgtaagtat taccgcgcat cgcactatac cccatggctg tgttgtcatg
     6241 cccggtattg gtcagcacca tcgcaccata acccatgctg gtattatacg agccggtagt
     6301 attcaactct cccgcgccaa aacccacggc ggtattatag gcgccgccat tattataata
     6361 aagggtataa ccgcctacgg ccacattctc gtatcccgga ttcgaaaaga gactcctgta
     6421 ccctatggcc gtgctgatag tgccgctgcc gttctgcgcc gccccatagc cgaatgccgt
     6481 catacccgtg acaccgagct gcccggaatt ggtattgttc accttgaatt gcaaagattg
     6541 attatccgtc gtcccgataa agttgacgga aggattggta cctgcattcc cggtaaggct
     6601 ccaggactgg gcccaggtag cagggacaag ggccagcccc aggatgagga gcgtcaattt
     6661 tttcatagcc cccaagttcc cccctttctg aaaattcctt atcacgtaaa ggagtgattt
     6721 tttaatacgg atttatttat aacctttacg gaccaaatcc ctctccccgc atgcaccgcg
     6781 ctcatcgata ccccctcctg gcggcaatca cgacgatctt attgatcgcc gcccccaccc
     6841 tgctgcctgc acaacccgac tcctaccagc gggcattcga gcgcgaccgc gattctgctt
     6901 tacgcgagct ccaaaaacac cccgaacccg atactgtccg tgca
//
LOCUS       DASYSV010000563         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_239094, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000563 DASYSV010000000
VERSION     DASYSV010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239094"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1312)
                     /locus_tag="VGQ51_17555"
     CDS             complement(56..1312)
                     /locus_tag="VGQ51_17555"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV9038449.1"
                     /translation="MKFKHTVNLLLALSCLSATAQQGQLYDNTNYKAIYFKEACDLIA
                     NTPGLLLLDVRSPGEYADTSQFIGSRIGRLKGAINISIDSILLHYRDLVPYKNRTILV
                     YCSHSQRSRGVSRLLGDSGFTKVYSLNGGMTEVDREPDASFPCKASLYTSNLPYRLMG
                     PEDAAVFIKDKNNVIIDIRPATQFNGTDTAEMTNIGRIKGAINIPGTELDQSIAGLEK
                     YKDRPILLYDLYTSNAMKAAARLTAAGFKKVAVLFNGFDTFLLNFSSASPIRKEWIMA
                     APRYQLTGVREAVDLVNHSPSLVVADMRAIDQFENKSKQKFFNLGHIRNAVNFQNAQQ
                     LEDYLKGKPKNTPVLIYGAFGPAMSSMNTDPVVDLPALSRRLAGEGYTRIHLLYAGIY
                     SLVWASANVDGQQDAKGILIDHEGLY"
     gene            1461..2006
                     /locus_tag="VGQ51_17560"
     CDS             1461..2006
                     /locus_tag="VGQ51_17560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEV9038450.1"
                     /translation="MGMPGADIGLRDQDPTVAEYLKVLGYATGQFGKNHLGDKDEFLP
                     TKHGFDEFFGNLYHLNAEEEPEDADYPKNPNFKKQFGPRGVLKCTSDGKIEDTGPLTK
                     KRMETVDEEFTSAAIDFMDRQVKAGKPFFCYYNSTRMHVFTHLSPEYEGKTGLGLQAD
                     GMAEHDDNVGKLQRRGQRGPP"
     gene            1963..2355
                     /locus_tag="VGQ51_17565"
     CDS             1963..2355
                     /locus_tag="VGQ51_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038451.1"
                     /translation="MWASCKGEVKEDPRNEFIYWSDDGDLFAIRYQRWKIDFIQQNHE
                     GLEIWSKGFEKLRLPYFYDLYADPFERGDKSIEYDVWMVHHAYLMYGAQALVGRWLQS
                     FREFPPRQKPASFNLDEVMRKMSEPQHN"
     gene            2412..>2772
                     /locus_tag="VGQ51_17570"
     CDS             2412..>2772
                     /locus_tag="VGQ51_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038452.1"
                     /translation="MLKNYFLVALRAFRRNKTFSFINIVGLSIGISASLVIFLLVQYD
                     FTFDKFEKDGSRIYRVVAEGHNDHNSWHGVSLSEPMGAAAKKDIPGLEIVAPFRTLDE
                     TRVTIPWPDAKHPIVFRK"
BASE COUNT          654 a          685 c          771 g          662 t
ORIGIN      
        1 tagcaatttt tttatttggc aattatcatt ttcattgcga tgagtcccgg catgatcaat
       61 acaacccttc gtgatcgatc aaaatgcctt tggcgtcctg ctgcccatcg acgttggcgg
      121 aggcccacac cagcgaataa atgccggcgt acagcagatg tatgcgggta tagccctctc
      181 ctgccagtcg ccgggaaagc gctggcaggt cgaccaccgg gtcggtgttc atggaactca
      241 tagcaggtcc aaaagcgcca tagatgagga caggcgtatt tttgggtttg cctttcagat
      301 agtcctcgag ctgttgcgca ttttgaaaat tgacggcgtt ccggatatgg cccaggttga
      361 agaacttctg tttcgattta ttttcgaact ggtctatggc gcgcatatcg gccaccacca
      421 gactggggga atggttgacg agatcaaccg cctccctcac acccgtcagt tggtagcgcg
      481 gagcagccat gatccattct ttgcgtatcg gcgaggcgga ggaaaaattg agcaggaagg
      541 tatcgaagcc gttaaagagc accgccacct ttttaaaccc cgcggctgtc agtctggccg
      601 ccgccttcat cgcattggaa gtgtacaggt catagaggag aatcggccga tccttgtatt
      661 tttcgagacc ggcaatggat tgatctaact ccgtcccggg tatgttgatc gcgcctttta
      721 tccgtccgat attggtcatc tcggcggtgt ccgtgccatt gaattgggtg gcggggcgga
      781 tatcgatgat cacattattc ttgtctttga tgaagactgc ggcatcctcc ggccccataa
      841 gccggtaagg gagatttgag gtgtataatg acgccttgca ggggaatgag gcatcgggct
      901 cgcggtcgac ctcggtcatt ccgccattca ggctgtatac cttcgtgaac ccgctgtctc
      961 cgagcaatcg gctgaccccc ctgctacgct ggctgtgtga gcaataaacc aggatcgtcc
     1021 tgttcttata cggcacgaga tcccgatagt gaagaaggat cgaatcgatg gagatattga
     1081 tggcgccctt gagtctgccg atccgggaac cgatgaactg gctggtatct gcgtattcgc
     1141 cgggcgagcg gacgtccagc agcagcagac cgggggtgtt ggcgatgagg tcgcaggcct
     1201 ctttgaaata gatggccttg taattggtgt tgtcgtatag ctggccttgt tgcgcggtgg
     1261 cggaaagaca gctcagggct aaaagcaggt tgacagtgtg tttgaatttc atgcggagga
     1321 ggttggtgtt cggacaaatg taggaaggcc tgtcgccccg ccctaccaaa atggagtaaa
     1381 agccgtattt acaggagtga agtgatgagc ggcgcgtgtt cccgacggga cagtcgccta
     1441 tccgaacagg attgaccaaa gtgggaatgc ccggagcgga tatcggcctt cgtgaccagg
     1501 acccgacggt cgccgaatac cttaaggtcc tcggctatgc tacaggtcaa ttcggtaaga
     1561 accaccttgg agacaaggat gaattcctgc ctaccaaaca tggtttcgat gaattcttcg
     1621 gcaatcttta ccatctcaat gccgaagagg agccggaaga cgccgattat ccgaaaaacc
     1681 cgaatttcaa aaagcaattt ggtccacgcg gcgtgctaaa atgtacttcg gatggaaaga
     1741 tcgaagatac cgggccgctg acaaaaaaaa ggatggagac tgtggatgag gaatttacgt
     1801 ctgccgctat cgattttatg gaccggcagg tgaaggcggg aaaacccttt ttctgttatt
     1861 ataattcgac ccggatgcat gtctttaccc atctcagtcc ggaatatgaa ggtaagaccg
     1921 gtctcggtct gcaagccgac ggcatggcgg agcacgatga caatgtgggc aagctgcaaa
     1981 ggcgaggtca aagaggaccc ccgtaacgag ttcatttact ggagtgatga cggggatctt
     2041 ttcgcgatca ggtatcagcg ttggaagatc gatttcattc agcagaatca tgagggtctc
     2101 gagatctggt caaaaggttt tgaaaagctg cggctgccct atttctatga cctctacgca
     2161 gatccgttcg agcgtgggga caaatcgatc gaatacgacg tctggatggt ccatcatgct
     2221 tacctgatgt atggcgcgca ggcgctcgtg gggcgatggc tgcaaagttt cagggaattc
     2281 ccgccgcggc aaaaaccggc aagtttcaac ctggatgagg tgatgcggaa aatgagcgaa
     2341 ccgcagcata attaacgcgt ttctacggtt ttggcatata tttatcctgg aagacaaaac
     2401 cgggactaaa tatgctaaaa aactatttcc tcgtcgcgct gcgcgcgttc cgccgcaaca
     2461 agactttttc tttcatcaat attgtcgggc tctccatcgg catcagcgct tcgctcgtca
     2521 ttttcctatt ggtgcaatac gatttcacct tcgataaatt cgaaaaggac ggaagccgca
     2581 tctaccgggt tgtcgccgaa ggccacaatg accacaacag ctggcatggt gtctctctct
     2641 ccgaacccat gggcgcggct gcaaaaaagg atatccccgg cctggaaata gtcgcgcctt
     2701 tccgtacgct cgatgagacg cgggtcacta ttccctggcc cgatgccaaa caccctattg
     2761 tcttccgcaa ac
//
LOCUS       DASYSV010000564         4475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1011659, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000564 DASYSV010000000
VERSION     DASYSV010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011659"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VGQ51_17575"
     CDS             complement(<1..589)
                     /locus_tag="VGQ51_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEV9038453.1"
                     /translation="MSNPIVAQSSLNQLSYLRNRYPLHPKAYLSLPFGTIKPKAWLFD
                     QLVSMKNGMCGNLDKLYPAVLGKRNGWLGGDGDVWERGPYWLDGLAPLAFILDDAGLK
                     AKLQPWIEWSIANQQPDGYFGPMPPKKEPAPEPGLQRDRARDWWPKMVMLKVLQQYYL
                     GTGDQRVIQLMLRYARYQLKTLPDTPLGHYSWWGAQ"
     gene            788..1807
                     /locus_tag="VGQ51_17580"
     CDS             788..1807
                     /locus_tag="VGQ51_17580"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV9038454.1"
                     /translation="MSKQNITIKELAQRLNVSIATVSRALRNTGDVNRDTRERVVRLA
                     TELHYSPNYFAQSLVKQQTKIIGVVVPTIYSHYFSQALCGMTDVANENDYYLMFCQSN
                     ETAALERKSMEKLLACHVDGLLISISKETKDSSVFEEVLNHGIPIVQFDRILAGLPVT
                     RVIVDEFEGAFKAVEHLIRRGCRRVAHFCGPTELSVSANRLNGYVEALKKHGIAVEKK
                     LIIPCNSFEDDAQIATKKLMRLKTLPDGLFAVNDLSAIAAIQYLKKHGIHVPQDIKVV
                     GFNDDPASRLIDPTLTTVMQPGYEVGKLAIGVLIDEIKNKKNKNRVFKLRTKLISRES
                     SRNPV"
     gene            complement(1760..3256)
                     /locus_tag="VGQ51_17585"
     CDS             complement(1760..3256)
                     /locus_tag="VGQ51_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV9038455.1"
                     /translation="MENFREESLDPANWDEMRRLGHEMVDTMMDYLQAVGQRPAWQKP
                     PEDIMKSLRQPVPMEGQDIREVYAEFMTKVLPYNLGNVHPRFWAWVQGGGTPVGMMAD
                     MLASGMNSNLAIGDQAPVYIERQVLDWSKELFGFPLEAGGILTSGASMANITALVVAR
                     NQFSASIKRSGLQDQGGILTIYGSAETHNCLIKGVEAIGIGSDHFRQVPVDGNFMIRT
                     DLLKKMIAADRRKGYQPMCIVGNAGTVNTAAVDDLEALADIARQEKCWFHVDGAFGAI
                     PNILPEWKPRLKGLEHADSLSFDFHKWLYMNYEVGCVLIRDGAAHREAFSAPASYLLT
                     HERGMAAGPEPFATYGLELSRGFKALKVWMLLKEHGIEKYSRMVRQNLEQAQYLGSLV
                     QGSAELQLLAEVSMNIVCYRWNPGGIGGDQLNAVNKEILMRLQEEGIAAPSYTLLDGK
                     YAIRVCITNHRSRREDFEVLVRETMRVGREVVGSNRVPARFAGDEFRS"
     gene            3558..3968
                     /locus_tag="VGQ51_17590"
     CDS             3558..3968
                     /locus_tag="VGQ51_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038456.1"
                     /translation="MWGTISDYMNSFLGAFALLLSLIFIPEIKQKKKYVIGVLGLIII
                     LVGLGIDKTNRDNVKDEKNAQQRKTDSLTIDSLKTDLRALAIEHGNDTIRWAKIERTL
                     LDSFHIDISNGTPTKVTYTYNTHFRDAGTVNIGR"
     gene            complement(3965..>4475)
                     /locus_tag="VGQ51_17595"
     CDS             complement(3965..>4475)
                     /locus_tag="VGQ51_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038457.1"
                     /translation="EFRVRVIRATSYCMKILLAFVVIILSLPAIAQQKNQYNTQIDSA
                     GTVNIYGNGTTIIGKIIVAAQSKLYLSSISHKQVAIDTVETLIKFETDGFYPIYSPEI
                     ILRFDKRIYSMDWGGEGSGYMTSAGIHTDSLGGIWKAQQFTGRLLNAVIRSKVPLFVS
                     VYGVARKRN"
BASE COUNT         1118 a         1248 c         1025 g         1084 t
ORIGIN      
        1 gctgtgcacc ccaccaggag taatgcccca acggggtatc gggcagcgtt ttcaattggt
       61 agcgcgcata gcgtagcatc aattgtatga ctctctggtc gccggttccg agataatatt
      121 gctgaagtac tttcaacatc accatcttcg gccaccagtc cctggccctg tcccgttgca
      181 gacccggctc cggcgcaggt tcctttttcg gcggcatcgg accaaaatag ccgtcgggtt
      241 gctggttggc gatcgaccac tcgatccatg gctgcagttt agccttcagc ccggcatcgt
      301 cgagaataaa agccagggga gccaacccgt ccagccaata gggtccgcgc tcccagacgt
      361 ccccgtcgcc acccagccaa ccgttccttt tacccagaac ggcggggtat agcttgtcga
      421 gattaccgca catgccattc ttcatggaca ccagctgatc gaatagccag gctttaggct
      481 tgatagtccc gaagggtaag gacaaatagg ctttcggatg cagagggtat cggttgcgca
      541 gataagaaag ttgattcagg gaggattggg caacaatagg attactcata aatagtagtt
      601 tggcaacaat caatagggac cattttttca tttggcggcg ttaattattt cttagctcga
      661 gattttcagt ggttcgaaat tagcgcaatc tcatccggaa agtctgcttc cgggcgttaa
      721 attgccagcc ggcgaccgta aacgttttcg gaaaaaaagt atttttgtat gctaccttga
      781 gcaacaaatg agcaaacaga acatcaccat aaaggagctg gcgcagagat tgaacgtctc
      841 gatcgcaaca gtctcgagag cgcttcggaa tacaggtgac gtcaaccgcg atacaaggga
      901 aagagtggtt cggctcgcta ccgagttgca ttattcgccg aattatttcg ctcagagcct
      961 cgtcaaacag cagacgaaga tcatcggggt cgtcgtgcca acgatctact cgcattactt
     1021 ctcgcaggcg ctgtgcggga tgacggatgt agccaacgaa aacgattatt acctcatgtt
     1081 ctgccagagc aacgaaaccg ccgcgctcga gcggaagagc atggagaagt tgctcgcctg
     1141 tcatgtcgat ggattactca tctctatatc caaagagaca aaggacagtt ccgttttcga
     1201 agaagtgctt aatcacggaa taccgattgt acagttcgat cgtattcttg ccggcctgcc
     1261 ggtgacccgg gtcatcgtcg atgaattcga aggggcgttc aaggcagtcg agcacctcat
     1321 ccggagagga tgccggcgcg tcgcgcactt ctgcgggccg actgaattgt cggtgtcggc
     1381 caatcggctg aatggttatg tcgaggcctt gaagaagcac gggattgccg tcgaaaaaaa
     1441 gttgatcatc ccctgcaact ccttcgagga cgacgcccag atcgccacca aaaaattgat
     1501 gcggctgaag accctgcccg acgggctgtt cgccgtcaac gatctctccg ccatcgccgc
     1561 gatccaatac ctgaaaaagc acggcattca tgtcccgcaa gatattaaag tggtaggttt
     1621 caacgacgac cccgcgagcc ggttgatcga tcccacattg accaccgtga tgcagccagg
     1681 ctacgaagtc ggcaagctgg cgatcggggt cctcatcgac gagatcaaaa acaagaagaa
     1741 caagaaccgc gtattcaaat taagaacgaa actcatctcc cgcgaatcga gcaggaaccc
     1801 ggtttgatcc gaccacctct ctccctaccc tcatcgtttc cctcaccagc acctcgaaat
     1861 cctctctcct gctccggtga ttggtgatac acacgcgtat cgcgtatttc ccatccagca
     1921 gggtgtacga cggcgccgcg atcccctctt cctgcagccg catcaatatc tccttattca
     1981 cagcattcag ctgatcgccg ccgatgcccc cgggattcca tctgtaacaa actatattca
     2041 tagacacctc cgccagcagc tgcagctctg ccgacccctg aaccagcgat ccgagatact
     2101 gcgcctgttc gagattttga cgcaccatcc ggctatattt ttcgatgcca tgttccttca
     2161 gcaacatcca aaccttgagg gccttgaatc ccctcgacag ctccaaccca taggttgcaa
     2221 acggctcagg ccccgcggcc atcccgcgct catgcgtcag cagatatgag gctggcgccg
     2281 aaaaagcctc ccggtgcgcc gccccatccc ggatcagtac gcacccgacc tcataattca
     2341 tgtacagcca tttgtggaag tcgaagctga ggctgtcggc atgctccaaa cccttcagtc
     2401 gaggcttcca ttcaggaagg atattcggaa tggccccgaa ggcgccgtcg acatggaacc
     2461 agcatttttc ctggcgcgcg atgtccgcca gcgcctcaag gtcatcgacc gcagcggtat
     2521 tgaccgtccc cgcattgccc acgatgcaca tcggctgata acccttccgg cggtccgcgg
     2581 cgatcatctt cttcagcaga tcggtccgga tcatgaagtt gccgtccacc ggcacctgcc
     2641 ggaaatgatc gctgccgatc ccgatggctt ccacaccttt gatcagacag ttgtgcgtct
     2701 ccgccgatcc gtatatcgtg aggatgccgc cctgatcctg cagccctgac cgtttgatgc
     2761 ttgccgagaa ctggttcctc gccacgacca gcgcggtaat attcgccatc gaagctccgc
     2821 ttgtgagtat cccgcccgcc tccaacggaa aaccaaacag ctccttgctc caatccagca
     2881 cctgccgctc gatatacacc ggcgcctgat cgccgatcgc caaattcgag ttcatcccac
     2941 tggcgagcat atctgccatc atccccaccg gcgtgccgcc accctgcacc catgcccaga
     3001 aacgcggatg tacattgccc aggttatatg gcaacacctt cgtcatgaat tcggcatata
     3061 cctcccgtat gtcctgaccc tccatcggta ccggctgccg caaactcttc ataatgtcct
     3121 ccggaggctt ttgccaggcc ggccgctgtc ccaccgcctg cagataatcc atcatcgtat
     3181 cgaccatctc atgccccagc cgccgcatct cgtcccaatt cgccgggtcc agactttctt
     3241 ctctaaaatt ttccatgatg cctattttga tcccgcaaag ctccatcttt ttgggttaca
     3301 aaacagatgc agttttgtca atatcggcta gatcagatgt tcgctgccgg tagtgtctca
     3361 gtttcgatgt ttcaggtttt caatgtgttg tgtaaaaaca ctacaaatac cttgaaaagt
     3421 tggcacgtta agacccatta tcgtttagcc atctggctac taattagaac aggaactaca
     3481 caaatcttca aacagggtta aaagaccttt cattctttaa ccactccctg ctaatttagc
     3541 attctaaaat tctaagtatg tggggaacaa tctcagatta tatgaactca tttctagggg
     3601 cattcgccct tctgctgtcc ttgattttta ttcctgaaat aaaacaaaaa aagaaatatg
     3661 ttatcggtgt cctaggttta ataataattt tagttggcct aggaatagat aaaaccaatc
     3721 gtgacaatgt gaaagatgaa aagaacgcgc agcagagaaa aacggattcg ctcacaatag
     3781 attcgttaaa gacggattta cgtgctctag ccatcgaaca cggcaacgac acaatccggt
     3841 gggcaaaaat agagaggacg ttgcttgata gctttcacat tgatatttca aatggcactc
     3901 cgaccaaagt gacatacact tacaatacgc attttagaga tgctggaacg gtaaacattg
     3961 gtcgttaatt tctcttccgt gcaacgccat aaaccgacac gaaaagagga accttacttc
     4021 gtattacggc gtttagcagt cttccggtaa attgttgggc cttccaaata ccaccaagcg
     4081 agtctgtatg tattccagct gaagtcatat atcccgatcc ttcaccgccc caatccatgc
     4141 tataaattcg tttatcaaat ctgagaataa tttcaggact ataaatcgga taaaagccgt
     4201 ctgtctcgaa tttaatcaaa gtttccaccg tatctattgc cacttgcttg tgtgaaatac
     4261 ttgatagata taattttgac tgtgctgcta ctattatctt cccgattatt gtagtgccat
     4321 tgccataaat gtttacagtt cctgcgctgt caatttgcgt attatactga ttcttctgct
     4381 gggcaattgc cgggagagat agtattatca caacgaaagc aagtagaatt ttcatgcaat
     4441 atgaggtggc tcgaattacg cggacacgaa attct
//
LOCUS       DASYSV010000565        16697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_497779, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000565 DASYSV010000000
VERSION     DASYSV010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 16697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16697
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497779"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VGQ51_17600"
     CDS             <1..997
                     /locus_tag="VGQ51_17600"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV9038458.1"
                     /translation="FRFMKPNIAFVTGGYSGESVISYNSAITIQNNLDYDRYTVYRID
                     ITPEGWFHPAEDGRKIPVDKNDFSITIGEKIRFDAILIGIHGTPGEDGKLQGYFDMLG
                     LPYSSCNAAVSAVTFNKRFTVALAAFSGIHVARSVLLIKDRIMSPDEVTRRLQFPVFV
                     KPNNGGSSIGMSKVNAPSEELGAAIEKAFREDSEVLVEEFIAGREFTIGVFRSKGEII
                     TLPFTEVKSKKEFFDFEAKYTAGLNEEITPAQVDEAVAVKVRAAAKRIYQVFNCRGII
                     RIDFIYNEEKGEPFMLEVNTVPGQSEASIVPQQLRAAGWSLKDFYSALIEEALAR"
     gene            1049..1873
                     /locus_tag="VGQ51_17605"
     CDS             1049..1873
                     /locus_tag="VGQ51_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEV9038459.1"
                     /translation="MFKFLTSKPLWVNIVAGIVLLLLLLLLFLGSLALLTQHGHTMKI
                     PSVTGMSYSEAKKTLESAGFDVQIQDSMYNDTMRPLQVVKQFPEADNLVKVNRTIYLT
                     INQSQAPIIQMPNLVSMTFRNAQAVLRQYGLKVGDTIFKPDYARNSVLDQLYHGSTIK
                     PGTQIQEGSSITLVLGTGIGSGQGFIVPDLTGLTYLQAKARLDSMGLIFGVVMPDKEV
                     RDSSESYIYWQEPKQFNDEGQVNRIRPGQAIDIRLGIQKPVPGTDSAHTGPAPAAY"
     gene            1905..2303
                     /locus_tag="VGQ51_17610"
     CDS             1905..2303
                     /locus_tag="VGQ51_17610"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV9038460.1"
                     /translation="MQAISVEEVKTRLDAGETLHLLDVREPAERAEFNIGGEFIPLGK
                     IRMMDTDGIDSWKSEEVICYCRSGNRSGQACMILESAGFTNVRNLTGGMLAWVNRFGS
                     LWSRPATPAAVLPQIFPKPGQPPIEPVALI"
     gene            complement(2244..4238)
                     /locus_tag="VGQ51_17615"
     CDS             complement(2244..4238)
                     /locus_tag="VGQ51_17615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV9038461.1"
                     /translation="MLKSFLLLSLTAAGLLTHAQSPAKRPMRPADMYRLPSVSDAQPS
                     PDGKWVSYTLSAIDSLKDSRNSDVWMISRDGSQDIQLTNSPDGESRARWSPDGKWLSF
                     LSGRTSSPPDPKGAQVWLMDRRGGEGRRLTTFKGGVGDYAWSPDSKKLLLTLTDPDPE
                     DTGKIKTTKPYVIDRYQYKEDVEGYRYKKVHTHLYLFDIATKKLDTLTRGDRDEESAV
                     WSPDGQSIAFVSNRSEDPDKNENTDIFVIDARPGASMRRLTDWKGSDNGPRWSPDGKS
                     IAYTRSVSDEDYHMYDEPVLCVIPAAGGEPRFLSRQLDRPVFNPRWSPDGRSILALVA
                     DNREQYVSRFPVGGGAITPIAQGQRVFNSLEQMGDGWITLSSEPQTPTEIYVMAGDGQ
                     TRRLTHHTDAFLEPLELAKVIPFQSKSADGTLISGQYFLPPGLPAGEKHPTILYIHGG
                     PVGQDAFDFDLSRQMLAAGGYVVVAVNYRGSSGRGVEFSKTISADWGNKEVLDLHGAV
                     DWLVKQGIADSTRLGVAGWSYGGILTDYLIASDTRFKAASSGAGTGFTLSLYGVDQYI
                     NQYNNELGPPWKSIDRYLKISYPLLKADRIRTPTLFLSGEKDFNVPTAGSEQMYQALR
                     TLGVPTELIIYPGQFHGISIPSYQRDRLDRWLAWFRKYLR"
     gene            complement(4247..6502)
                     /locus_tag="VGQ51_17620"
     CDS             complement(4247..6502)
                     /locus_tag="VGQ51_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEV9038462.1"
                     /translation="MKNLLALLCLMGIITVSYAQTDTTRRTYDSLKLFLQPVEVRALR
                     AGEKAPFTKTDINKKQIEETNVGQDLPLLLNQTPSVMINSDAGNGVGYTGIHIRGTDD
                     SRINMTLNGIPYNDPEEQALFFVDIPDLASSVGSIQIQRGVGTSSNGAGAFGATLNFS
                     TNEFNEKPYAEFNNSYGSFKTWKNTLRAGSGLIDGHFTVDMRLSRVSSDGYIDRATSD
                     LKSLYFSAAWYDKRSSIRFNLLQGTEKTYQAWNGIPQAKWEGNLPALQQHYENNVGSL
                     YFNSQDSLNLFRSSNNTYNYFTYKNQTDNYWQNHYQLFFNHRWSDALALNSAIFLTRG
                     YGYYEEYHNQGDSANAKYTAYGLPPFEAGNVVFQSTDLIRQRWLDNYFYGTVLSLQYK
                     QNNTQLVLGGGWDRFDNKHYGYVLWAANGGFPDNYRYYWEPAIKIDYNVYAKWQQQLG
                     ERWTTFADVQYRHLDYEVDGFDDNPSILVHPKYDFINPKIGVTYTHHDWQAYFSYSLA
                     SHEPNRDDFEAGLTEQPRPEMLHDLELSLQKKAPLYSWSITGYYMRYHDQLVLTGKIN
                     DVGGYTRTNIPRSYRLGIELQGNIRPSDWFTADANLALSRNKVFDYTEYDDDYDNGGQ
                     VSHGYKTTDISFSPDIVSAATVNFIPTKNLTVSLPAKYVGKIYLDNAQQEDRKLDGYY
                     VQNLRLLYKFTHGPGKETDLIFQLNNVFNKRYTPNGYTYSYVYGGQLVTENFVFPMAG
                     TNFMLAVNIKL"
     regulatory      complement(6553..6657)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6769..7491
                     /locus_tag="VGQ51_17625"
     CDS             6769..7491
                     /locus_tag="VGQ51_17625"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEV9038463.1"
                     /translation="MRNILLLTAFLFAGRLGFAQDGKVINDRNAQKRNAAGFHGIHIS
                     SGIDLYLTQGNEEAVAVSAADPEVRNRIVAEVKNGVLEIYIDNQGWHWNHWGNQHMKA
                     YVSCKTIDMLHASGGSDVYFESPLRSDRLTMDLSGGSDMHGKMAVGELTIRQSGGSDS
                     YISGSATNLYVHTSGGSDFHGYDLIADNCHVEATGGSDAYITVNKELTADASGGSDVH
                     YKGNGVVRESHASGSGSISHRD"
     gene            7508..8218
                     /locus_tag="VGQ51_17630"
     CDS             7508..8218
                     /locus_tag="VGQ51_17630"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEV9038464.1"
                     /translation="MRKTYLLVVASLLVLTIRAQKTIVHDPNAEVRPLKGYHGIEVSD
                     GIDLYLSQGDIETVVVSARDIKMRDRIRTEVVDGILRISLPHGWTVHDYKLKAYVSFT
                     TLDRLTAAGASDVFVDGIIAGDRLVVNLSGASDFKGAVNVKQLKMEQSGASDARLTGV
                     VSDMASFSSSGSSHIKGYDLVTQSCAVDASGASDIQITVNKELSASLSGASNVYYKGT
                     GVIRETHFSGASAIKKVS"
     gene            8298..8369
                     /locus_tag="VGQ51_17635"
     tRNA            8298..8369
                     /locus_tag="VGQ51_17635"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8330..8332,aa:Met,seq:cat)
     gene            8448..9317
                     /gene="era"
                     /locus_tag="VGQ51_17640"
     CDS             8448..9317
                     /gene="era"
                     /locus_tag="VGQ51_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV9038465.1"
                     /translation="MKSGFVSIFGKPNAGKSTLLNALMGEKLAIVSPKVQTTRHRIKG
                     ILTEKDYQVIFSDTPGIIEPRYKLHEKMMQAVRNSIEDADLALLLVDVRDDWEENDRL
                     FTALKLGSPAILVLNKSDESSQEKVEEARSFFSARAYAREVIAISALKKKNIDGLLAA
                     VVRLLPEGHPFFDGDDLTDLPTKFFVGELIREKIFLLYADEIPYQATVLVQEFKEKTT
                     LTKVRADIIVQRETQKGIILGEGGKMIRQLGSDARADIEKFIGRKVFLELFVKVRPKW
                     RDTETYLKEYGYN"
     gene            9319..10629
                     /gene="der"
                     /locus_tag="VGQ51_17645"
     CDS             9319..10629
                     /gene="der"
                     /locus_tag="VGQ51_17645"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV9038466.1"
                     /translation="MSGFTVAVVGRPNVGKSTFFNRLLEQRKAIVDDVSGVTRDRQYG
                     VAEWAGRNFNLIDTGGFVPDSEDIFETEIRKQVLIALDEANALIFMVDAATGITALDE
                     AMADVLRRTTKPVFLAVNKVDNSERLLEAAEFYSLGFDEIYFLSSMTGSGTGELLDAL
                     TALIKEEQTSTEDEGLPKFAIIGQPNVGKSSLLNALIGQERTIVSEIAGTTRDTIHTR
                     YNLFKKDFILIDTAGIRRKTKVQEDLEFYSVIRAIKAMDEADVCLLMLDAGKGITAQD
                     LNIFSLAVKKGKGIVILVNKWDVIEKETNTAREYEKELKQRLAPFTDVPILFISVIDK
                     TRIFRAVESALEVYENRQRKVTTAVLNDVMLAAVEAYHPPVVRGAPLKIKYVTQLPTA
                     VPSFAFFCNFPDDVKQPYKNYLENKLRENFNFKGVPVRLFFRKK"
     gene            complement(10631..12853)
                     /locus_tag="VGQ51_17650"
     CDS             complement(10631..12853)
                     /locus_tag="VGQ51_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HEV9038467.1"
                     /translation="MNEIKKNPIHSLGYGFIENRYLPKDKDEYYLRNLQNRSGIPYRQ
                     LTAYEIEVLVRNRNTSANWNDILVSDAFTPELVQNCKFFGLVRIGKLDPWYLEFHNLR
                     RPVGLYNSTIISCDLGDNVCIDNTNYLSHYVIGNEVMIVNTNEMATTDHAKFGNGILK
                     DGEDESIRIWLEVCNENGGRSIIPFNGMLPGDAWLWSRHRDDKVLQEKFKEFTASAFD
                     HRRGYYGKVGDRTVIKNCKIIKDVWIGSDAYLKGANKLKNVTINSSPQAMSQIGEGCE
                     MVNGIIGFGCRAFYGVKAVRFVMASHSQLKYGARLINSYLGNNATISCCEVLNSLIFP
                     AHEQHHNNSFLCAALVMGQSNMAAGATIGSNHNSRSPDGELVAGRGFWPGLCVSLKHN
                     SRFASFVLLAKGDYPAELDIPVPFALVSNDVSKDELVVMPAYWFLHNMYALARNSWKY
                     VDRDRRTEKTQLLEFDYLAPDTVGEMVHALAIFERCTGQAIARASGKTTKYTDEQLRE
                     MGAALLEHSPGEADKLEILADAMENSDRKVRLVKVAQAWSLFRELIAHHASNQLLRCI
                     ADKDHEIRSYAELLKALPSNGSMHPWVNAGGQLIRQPELDKLIAQIHNGKIKTWQSVH
                     EWYVRQGQQYPADKLTHALAALKTATGVSLRRDGPKVLGHLLRQSVALKEQLTKEIYG
                     SRSKDYSNPFRKMVYESPEEMNAVMGRLSDNSFIKQEKEATKKYRATVEKLVAEWKLE
                     "
     gene            complement(12857..14692)
                     /gene="glmS"
                     /locus_tag="VGQ51_17655"
     CDS             complement(12857..14692)
                     /gene="glmS"
                     /locus_tag="VGQ51_17655"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV9038468.1"
                     /translation="MCGIVAYIGPREAYPIIMKGLKRLEYRGYDSSGVALLNNNLRVY
                     KKKGKVADLEDSLVGSDLHAHAGMGHTRWATHGEPNDRNAHPHSSASGRLAMIHNGII
                     ENFTQLKNELIRKGYKFSSDTDTEVLLNFIEDIQRNNDCGLEEAVRIALKRVVGAYVI
                     VLLDKDAPDTVIAARKGSPLVIGIGKGEHFLASDASPIIEYTKEVVYVNDYELAILKA
                     DELVLKNLGNERITPYIQKLDLELASIEKGGYDHFMIKEIFEQPDTIFDCLRGRLDAA
                     KGTITMAGIQQNMDKLKNANRIIMIACGTSWHAGLVAEYVFEELCRIPVEVEYASEFR
                     YRNPVIHPGDVIIAISQSGETADTIVAIEKAKENGAFILGVVNVVGSSIARISNAGAY
                     THAGPEIGVASTKAFTAQLAVLTMIALKIAHEKGTIEPTRYMQLLNELHEIPEKAKEV
                     LKLSPAIKQLAEKYKDAKDALYLGRGYNFAVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALVEETLPVIFVATKDSYHEKIISNIQEIKARRGKVVAVITQGDSIISKMADDMIP
                     IPETDELIAPMLTVIPLQLLAYYTGVAKGYDVDKPRNLAKSVTVE"
     gene            complement(14805..15701)
                     /locus_tag="VGQ51_17660"
     CDS             complement(14805..15701)
                     /locus_tag="VGQ51_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038469.1"
                     /translation="MRPKLVMAIMLLTLAGMTGYAQRGGGHGGGGGHFGGGGHSFSGG
                     GGARGFSGGGARGFSGGGSRGFVGGGSRGYFGGGSRGFSGGSRGFSGGARVSSGYGGG
                     ARYGGGARGYIGTGRGYVTGRGGYNGHGGGYYSGGRYYSGGRYYGGHYYGGWGWGVGF
                     GLGYYGWPYSYGYPYYPYYYGYPSYSYPPSYYDPGYDEPADPYDDGSGYQQAPPQSIQ
                     PPPPPPAQNNTAPNNYYNNPYNYPNPDSLQNVPGTNQNAPTSYSNSGNAGDKIWVKAH
                     WKYTNNGWIWIDGYWKPSPANY"
     gene            complement(15813..>16697)
                     /gene="mgtE"
                     /locus_tag="VGQ51_17665"
     CDS             complement(15813..>16697)
                     /gene="mgtE"
                     /locus_tag="VGQ51_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEV9038470.1"
                     /translation="IDVIYVVDDKGYFIDDIRIREIILANPEKKIEDVIDNRYIALSV
                     NDAQETASEAFKMNNRVALPVLDNNNILLGIVTIDDVLWITQEEFSEDIQKIGGTEAL
                     DEPYLEMPLLKLFRKRIVWLVVLFLSEMLTATAMAYFEDEIAKAVVLALFVPLIISSG
                     GNSGSQASTLIIQAMALGELTILDWWRVMRREILSGLLLGAVLGVIGFTRVILWNQIF
                     HTYGEHGVLIALTIGVALIGVVMWGTIAGSMLPIVLKKFGADPATSSAPFVATLVDVT
                     GLVIYFSVAFLIMRGILL"
BASE COUNT         3707 a         4329 c         4672 g         3989 t
ORIGIN      
        1 ttttcgtttc atgaagccca atatcgcctt cgtcaccggc ggatattccg gtgaatcggt
       61 tatctcctac aacagcgcca ttaccattca gaacaatctg gattacgacc gctatacggt
      121 ttaccgcatc gatatcacgc cggaaggctg gttccatcct gcggaagacg gcaggaaaat
      181 cccggtcgat aagaatgact tctccatcac gatcggggaa aagatccgtt ttgacgccat
      241 tctcatcggc atccatggga ctccgggtga ggacggtaaa ctgcagggtt atttcgacat
      301 gctcggcctg ccgtacagca gctgtaatgc ggctgtcagc gcagtcactt tcaacaaacg
      361 gtttaccgtg gcgctggctg ccttcagcgg catccatgtg gcccgcagcg tacttcttat
      421 taaagatcgg atcatgagtc cggatgaagt gacgcgtcgc ttgcagttcc cggtctttgt
      481 caagcccaac aatggaggct cgagcatcgg catgtcgaag gtgaatgctc cgtcggaaga
      541 gctgggagcc gccatcgaga aggcgttccg cgaggacagt gaagtattgg tcgaagaatt
      601 catcgcaggc agggagttca ccatcggggt atttcgttcg aaaggtgaga tcattaccct
      661 tccttttacg gaagtgaaga gtaaaaagga attcttcgat tttgaggcca agtatacggc
      721 gggacttaat gaagagataa ccccggcgca ggtggatgag gcggtggcag tcaaggtgcg
      781 ggccgcggct aaaaggatct accaggtatt caattgccgg gggatcattc ggatcgattt
      841 tatctacaat gaggaaaagg gtgagccttt catgctggaa gtcaatacgg taccgggaca
      901 gagcgaagcc agtatcgtgc cgcagcaact gagggcggcg gggtggtcgc tgaaggattt
      961 ttattcagcg ctgatcgagg aggccctggc gcgttagccc aaatttcttt atcttcggct
     1021 gtccgtccat tgttaaaaac catttatagt gtttaaattt cttacttcca agccgctgtg
     1081 ggtcaatata gtagcaggaa ttgtgttgct gctcctcctc ctgctgctct tcctgggttc
     1141 gctggctctt ctgacccagc atggccatac gatgaaaata ccctccgtca ccgggatgtc
     1201 ctattccgaa gccaaaaaga ccctggaatc ggcaggtttc gatgtgcaga tacaagactc
     1261 gatgtacaac gataccatgc gcccgctgca ggtggtaaaa caattccccg aagccgacaa
     1321 cctggtcaag gtcaacagga ccatctacct tacgatcaat cagtcgcagg cgcctatcat
     1381 ccagatgccc aacctggtaa gcatgacttt ccgcaatgcg caggccgttc tccggcaata
     1441 cgggttgaaa gtgggagata cgatcttcaa gccggattat gcgcgcaact ctgtccttga
     1501 ccagctatat cacgggtcga ccatcaaacc cggtacgcag atccaggagg gcagctccat
     1561 tacgctggta ctcggcaccg gcatcggaag tggtcagggt tttatcgttc cggatctgac
     1621 cgggctgacc tatctccagg ccaaggcccg tctggactcc atggggctca tttttggcgt
     1681 ggtcatgccc gacaaggagg tcagagattc gtctgagtcc tatatttatt ggcaggaacc
     1741 caaacagttc aatgacgagg ggcaggtcaa ccgcattcgt cccggccagg ccatcgatat
     1801 ccggcttggg atacagaagc cggtgccggg gaccgattcc gctcatacag ggccggcgcc
     1861 ggccgcttat tgattatata tataccattc aaagcaatct tattatgcaa gctatttctg
     1921 ttgaggaagt caagacccgg ctcgatgccg gcgaaaccct gcatctcctg gatgtccgcg
     1981 aaccggctga acgcgccgaa ttcaacatcg gcggcgaatt tataccgctg gggaaaatcc
     2041 gcatgatgga caccgatggt atcgatagct ggaagagcga ggaggtcatc tgctattgtc
     2101 gcagcggcaa ccgcagcggt caggcatgta tgatcctcga gtcggcaggt tttaccaacg
     2161 tcaggaacct tacgggcgga atgctcgcct gggtgaatcg ttttggctcc ttgtggtcgc
     2221 ggccggctac gccggccgcg gttttaccgc aaatatttcc taaaccaggc cagccaccga
     2281 tcgagccggt cgcgttgata tgaagggata ctgatcccat ggaactggcc gggatagatg
     2341 atcagctcgg tcgggacccc gagggttcgg agcgcctgat acatctgctc gctgccggcg
     2401 gttgggacat tgaagtcttt ctcgccgctg aggaacaggg tcggtgtcct gatcctgtcc
     2461 gccttcaata atggatagct gatctttaaa taccggtcga tggatttcca gggtggcccg
     2521 agctcgttgt tatattggtt gatatactga tcgacgccat agagcgaaag cgtgaatccc
     2581 gttccggcgc cgctgcttgc cgccttgaaa cgggtatcgg atgcgatgag atagtcggta
     2641 aggatccctc catagctcca tcccgccaca cccagccggg tggaatcggc aatgccctgc
     2701 ttcaccagcc aatctactgc accatgaaga tccaggactt ccttatttcc ccaatccgcg
     2761 gatatggtct tgctgaattc cacaccacgt ccgctgctgc cgcgatagtt caccgccacc
     2821 accacatatc cgcctgctgc cagcatctgc cggctgagat cgaaatcgaa ggcgtcctgg
     2881 cctacggggc cgccgtggat gtagagaatg gtcggatgtt tttcgccggc gggtagtccg
     2941 ggcggaagga aatactggcc ggaaataagg gtgccatcgg cgctttttga ctggaaggga
     3001 atgactttcg ccaactcgag aggttccagg aacgcgtcgg tatgatgggt cagccggcgc
     3061 gtttgtccgt cgcccgccat cacatagatc tccgtcggag tttgcggttc gctgctcagg
     3121 gtgatccatc cgtcacccat ctgctccagt gagttgaaga cccgctgtcc ttgggcaatc
     3181 ggggtgatgg caccaccgcc gaccggaaac cgggacacat actgctcacg gttatccgct
     3241 accagcgcaa ggatcgatcg tccatcgggc gaccatcttg gattgaatac ggggcgatcg
     3301 agctggcggc tcaggaagcg gggttcacca ccggctgccg gaatgacgca gagcaccggt
     3361 tcgtcgtaca tgtgatagtc ctcatcggag accgagcggg tataagcgat ggatttgcca
     3421 tccggactcc agcgggggcc gttgtcgctg cctttccagt cggtgagacg acgcatcgat
     3481 gcacccggtc gcgcgtcgat aacaaagata tccgtattct cattcttgtc ggggtcttca
     3541 gaacgattgc tgacgaaggc gatggactgc ccgtcgggac tccataccgc cgactcttcg
     3601 tcacggtccc cgcgcgtcag cgtgtcgagt ttttttgtcg cgatgtcgaa gaggtagaga
     3661 tgggtatgca cctttttgta ccggtagcct tcgacgtcct ccttatactg gtaacgatcg
     3721 atgacataag gtttggtggt tttgatcttg ccggtatcct ccggatcggg atcggtgaga
     3781 gtgagaagga gcttctttga atcggggctc caggcataat cgcctacccc tcctttgaac
     3841 gtcgtcagcc ggcggccttc accgccgcgg cgatccatca gccagacctg cgcgcctttc
     3901 gggtccgggg gcgaggaggt gcggcccgac aagaacgaga gccacttgcc gtcgggcgac
     3961 cagcgggccc gtgattctcc gtcgggggaa ttggtgagct gtatatcctg gctcccgtcc
     4021 cggctgatca tccagacatc cgaattacgg ctatccttca atgagtcgat agcagagagc
     4081 gtataggata cccatttgcc atcaggcgag ggttgtgcgt cgctgactga tggaagtcga
     4141 tacatatcgg cggggcgcat ggggcgtttg gccggggact gggcgtgcgt cagcaggccg
     4201 gcggcggtca ggctcaggag cagaaaggat ttcagcatag ctgaagttac aatttaatat
     4261 tcacagccag catgaaattc gtgcccgcca tggggaaaac gaagttctct gtcacgagct
     4321 gtccaccgta gacgtaacta tacgtatagc cgttgggggt atatcgtttg ttgaagacat
     4381 tgttcaactg gaagatcaga tccgtctcct tgccgggacc atgcgtaaac ttatatagca
     4441 agcggagatt ttgtacgtaa tagccgtcta gttttcgatc ttcctgctgc gcgttgtcga
     4501 gatagatctt gccgacatac tttgcgggca gactcaccgt gaggtttttc gtcggtatga
     4561 aattcaccgt ggcggcggat acgatgtccg gcgagaaaga gatatccgtt gtcttgtagc
     4621 catggctcac ctggccgcca ttgtcatagt catcgtcgta ttcggtataa tcgaaaacct
     4681 tgttgcggct gagcgccagg ttggcgtcgg cggtgaacca atccgacggc cggatgttcc
     4741 cctgcaactc gatccccagc cggtagctgc gcgggatatt ggtgcgcgta tagccgccta
     4801 cgtcattgat ctttccggtg aggaccaact gatcatgata acgcatataa tagccggtga
     4861 tggaccagga ataaagcggt gccttttttt gcaggctcaa ttcgagatcg tgcagcattt
     4921 cgggacgcgg ttgttcggtg agacctgcct cgaaatcatc cctgttaggc tcatggctgg
     4981 ccaacgaata cgagaaataa gcctgccagt cgtggtgcgt ataggtgacc ccgatcttgg
     5041 gattgatgaa gtcgtatttc ggatggacga ggatggaagg gttgtcgtcg aaaccatcca
     5101 cctcgtaatc gagatggcgg tattggacgt cggcgaaggt cgtccatctc tcgcccaatt
     5161 gttgctgcca tttcgcatag acattgtagt cgatcttgat ggcgggctcc caataatagc
     5221 gatagttgtc gggaaacccg ccattggccg cccacagcac atagccgtaa tgtttgttgt
     5281 caaacctgtc ccagccaccg cccagcacca gctgggtatt gttctgtttg tattgcagcg
     5341 atagcaccgt cccatagaaa tagttgtcca gccagcgttg ccggataaga tccgtgcttt
     5401 gaaaaacgac gttgcccgct tcaaagggcg gcagaccata ggccgtgtat ttggcattgg
     5461 ccgaatcgcc ctggttgtga tactcctcat aataaccata accgcgggtg aggaaaatcg
     5521 cgctgttcag cgccagcgca tcgctccacc ggtgattgaa gaataattga taatggttct
     5581 gccagtagtt gtccgtttgg ttcttgtagg tgaaatagtt gtaggtattg ttgctggaac
     5641 ggaaaaggtt cagcgaatcc tggctattga aatagagcga gccgacatta ttctcgtagt
     5701 gctgttgcag tgcggggaga ttgccttccc atttggcctg cgggataccg ttccaggcct
     5761 ggtatgtttt ttcggtgcct tgcaggaggt tgaaccggat cgaggatcgt ttgtcatacc
     5821 atgccgccga gaaatacagt gatttgagat cgctggtggc cctgtcgata tacccatcgc
     5881 tgctgactcg cgagagccgc atgtcaacgg tgaaatgccc gtcgatcagc ccggaccctg
     5941 ccctgagcgt atttttccag gttttgaagg acccgtagct gttgttgaat tcggcgtagg
     6001 gtttctcatt gaattcattg gtggaaaaat tcagggtggc gccaaatgcg ccggcgccat
     6061 tggacgaggt tccgacaccg cgctgtatct ggatgcttcc cacggacgag gcaagatcgg
     6121 ggatatcgac gaaaaagagg gcttgttctt ccggatcgtt gtaagggata ccgttcaatg
     6181 tcatgttgat tcgcgaatcg tcggtcccgc ggatatggat acccgtatag ccgacaccgt
     6241 tgccggcatc ggaattgatc atgacggagg gtgtttggtt gagaaggagg gggaggtcct
     6301 gtccgacgtt ggtttcttcg atctgtttct tattgatatc cgtcttggtg aagggcgctt
     6361 tttctcctgc acgcaaggca cggacttcga cgggttgcaa aaataatttg agggagtcgt
     6421 atgtacgccg ggtggtgtcg gtttgcgcat aagagaccgt aatgatcccc atcagacaaa
     6481 gcagagccag tagatttttc atcctgcaat agttttcgat gtcttaaaac gggaataccg
     6541 cgaaggggga gacagtagat tggagacctt ccctgcgcag gcattaccct gatcaggttc
     6601 tgcgggtatt tctcagccgt ccggcctttc ggcgggacag caccccgagg aactgaccac
     6661 aaagataggg gctttttcgc ctacggctgt aactttttca aaaggatggc cgtcccagag
     6721 gaggatgcaa catctactat ctcggtttgt ctttaaaaaa acatgattat gagaaatata
     6781 ctactcctta ctgcttttct attcgccggc cgccttggtt tcgcccagga cgggaaggtc
     6841 atcaacgaca ggaatgcgca aaagcgcaac gcggctggtt ttcatggcat ccatatctcc
     6901 agcggcatcg atctgtattt gacgcagggc aatgaggaag ccgtggcggt cagcgccgca
     6961 gatcccgaag tccgcaaccg gatcgtcgct gaggtaaaga acggcgtatt ggagatctat
     7021 atcgataacc agggctggca ttggaatcac tggggtaacc agcacatgaa ggcctatgtc
     7081 tcctgcaaga ccatcgacat gcttcatgcc tcgggcggat cggacgtgta tttcgaaagc
     7141 ccgctgagat ccgacaggct gactatggat ctttccggcg ggagcgatat gcatgggaaa
     7201 atggctgtcg gtgaactgac gatccggcaa tccggaggtt ccgactccta tatcagcggt
     7261 tcggctacca acctgtatgt ccataccagc ggcggcagcg attttcatgg ttatgatctc
     7321 atcgccgata attgtcatgt agaagcaacc ggcggcagtg acgcctatat caccgtcaac
     7381 aaagagctga cggccgatgc cagcggcggc agcgatgtcc actataaagg gaatggtgtg
     7441 gtgagagaat cgcatgccag cggatcggga agtatttccc atcgggatta atccttaaat
     7501 ttaccctatg cgtaaaacgt atcttttagt cgttgcgagt ttgctggtgc tgaccatccg
     7561 ggcgcagaag acgatcgtac atgatccgaa tgcggaggtg cgaccgctaa aaggatatca
     7621 cggcatcgaa gtatccgatg gcatcgatct gtatctgagc cagggagata tcgagacggt
     7681 agtggtcagc gcgagggaca ttaaaatgcg cgatcgtatc aggactgaag tggtcgatgg
     7741 cattctgcgg atcagtcttc cgcatggttg gacggtgcat gattacaagt tgaaggccta
     7801 tgtgtccttt actacgctgg atcgattgac cgcagccggg gcgagtgatg tgtttgtcga
     7861 cgggatcatc gcgggcgatc ggctggtcgt aaatctttcc ggggccagcg atttcaaggg
     7921 ggcggtgaat gtaaagcagc tcaagatgga acagagcgga gcttcagacg cccgtcttac
     7981 cggggtggtg tccgatatgg caagcttcag ctcttccggc tccagccata tcaaagggta
     8041 tgacctggta acgcagagct gcgccgtgga tgcttccggc gcctcggata ttcaaatcac
     8101 ggtaaataaa gagctttctg catcgctgtc cggcgccagc aatgtctatt ataaaggaac
     8161 gggggtcatt cgcgaaaccc acttcagcgg ggcaagcgct atcaaaaaag tctcctgaat
     8221 ccggccgccc ccggctcgga aaatatttcc atttatttgg ctgggaaggc aaagagtcgt
     8281 acctttgccc tccacatcgc gaagtagagc agcggtagct cgtcgggctc ataacccgaa
     8341 ggtcggtggt tcgatcccac ccttcgcaac aacgtctggg cctcgatgag gccctttttt
     8401 tatacctttg cggcccggcg ctgtagcgcc gggccgcgtc gacaactatg aagtccggat
     8461 tcgtctccat atttggtaaa ccaaacgccg ggaaaagcac cctgctgaac gccctcatgg
     8521 gtgaaaagct tgctattgtc tcgccgaaag tacagacgac ccgtcatcgc atcaaaggca
     8581 tcctgactga aaaggactac caggtcattt tttcggatac ccctgggatc atcgaaccgc
     8641 gatataaatt gcatgaaaag atgatgcagg ccgtccgcaa ttcgatcgag gatgctgatc
     8701 tggccttgct gttggtggat gtccgggacg attgggagga gaatgaccgg ctttttaccg
     8761 ccctgaaact cgggtcaccg gccatcctgg tgttgaataa aagcgatgag agcagtcagg
     8821 aaaaggtgga ggaagcacga agctttttca gcgccagggc gtatgcacgc gaagtgattg
     8881 ccatttctgc tttaaaaaaa aagaacatcg acggattgtt ggcggcggtg gtgcggttgt
     8941 tgccggaagg ccatcctttt ttcgacgggg atgatcttac cgatctgccg acaaaattct
     9001 ttgtagggga attgatacgg gagaagatat ttttgttata cgccgacgag atcccctacc
     9061 aggcgacggt cctcgttcag gagttcaaag aaaagacgac ccttacgaag gtgcgcgcag
     9121 acatcatcgt gcaacgggag acacagaagg gcatcattct cggggaaggc ggcaagatga
     9181 tccgccaact ggggtcggat gcgcgtgcgg acatcgagaa attcatcggt cgtaaggtat
     9241 ttctggaact tttcgtcaag gtacggccga agtggagaga tacggaaacc tatttaaagg
     9301 aatacgggta caattaatat gagtggattt acagtagccg tcgttggacg gcctaatgtg
     9361 gggaaaagta cgtttttcaa tcgcctcctc gagcagcgga aagctatcgt ggacgacgtc
     9421 agcggcgtca cgcgcgaccg ccagtatgga gttgcggaat gggccggaag aaacttcaac
     9481 ctcatcgata ccggcggatt cgtcccggac agtgaggata ttttcgagac cgagatccgc
     9541 aaacaggtgc tgatcgcact cgatgaggcc aacgccctga tctttatggt cgacgcagct
     9601 accgggatca cagctctcga cgaggcgatg gcagatgtgc ttcgtcgtac gaccaagccc
     9661 gtcttcctgg ccgtcaacaa ggtcgataac agcgagcgtt tgctcgaggc tgccgaattc
     9721 tatagcctgg gtttcgatga gatatatttc ttatcctcta tgacgggcag cggtacgggc
     9781 gaattgctcg atgcactcac ggcgctgatc aaagaggagc agacgtcgac ggaagatgaa
     9841 gggcttccca agtttgcgat cattggacaa cccaatgtag gtaaatcctc tttgctaaat
     9901 gcgcttatcg ggcaggaacg caccattgtc agcgaaattg ccggtacgac acgggatacc
     9961 attcataccc gatacaatct gttcaaaaaa gatttcatcc tgatcgatac tgccggcatc
    10021 cggcgcaaga ccaaagtgca ggaagacctg gaattctatt cggtgatccg cgcgatcaag
    10081 gctatggacg aagcggacgt ttgtctgctg atgctcgatg ccggcaaggg aattaccgcg
    10141 caggacctga acattttcag tcttgcggta aaaaaaggaa aaggcatcgt catccttgtc
    10201 aataaatggg atgtgatcga aaaggaaacc aatacggcca gggaatatga gaaagaattg
    10261 aaacaacggc tggcgccctt tacggatgtg ccgatcctct tcatttcggt catcgacaag
    10321 acgcgcattt ttagagccgt cgaatccgcc ctcgaggtgt atgagaaccg ccagcggaaa
    10381 gtgactacgg cggtgctaaa cgatgtgatg ctggctgctg tggaggccta tcatcctccg
    10441 gtggtgcgcg gggcgccgct gaagataaaa tatgtgacac agctgccgac ggcggtgccg
    10501 tcttttgcct tcttttgcaa tttcccggat gacgtgaaac agccgtataa gaattacctc
    10561 gagaataagt tgagggaaaa tttcaatttc aagggtgtgc cggttcgtct gtttttccgc
    10621 aagaaatagg ttattctaac ttccattcgg cgaccagctt ttccacggtc gcgcggtatt
    10681 tttttgtcgc ttctttttcc tgtttgataa aggagttgtc tgacagacgc cccatcaccg
    10741 cattcatttc ctcgggtgac tcatacacca tcttccggaa tggattgcta tagtctttcg
    10801 accgggatcc atagatctct ttggtaagct gttctttcag cgcgacgctt tgccggagca
    10861 gatggccaag taccttcggg ccgtcacgcc gcagcgagac accggtagcc gtctttagcg
    10921 cggccaaagc atgcgtcagc ttgtccgcgg ggtattgttg accctgacga acataccact
    10981 catgcaccga ctgccaggtc tttatcttac cgttgtggat ctgcgcgata agcttgtcca
    11041 gttcgggttg ccggatcaac tgtccgccgg cgttgaccca tgggtgcatc gacccgttgg
    11101 acgggagggc cttgagcaat tcggcatagc tgcggatctc atgatccttg tccgcgatac
    11161 accgtaatag ctggttggag gcatgatggg cgatcagctc tctgaaaagg gaccaggcct
    11221 gagctacttt caccagccgg actttccggt cgctgttctc catcgcatcg gccaggatct
    11281 ccaatttgtc cgcctcaccg gggctatgct cgagtaatgc ggcgcccatt tcgcgaagct
    11341 gttcgtcggt atattttgtc gtctttcccg acgcccgggc aatggcctgg cccgtacacc
    11401 gctcgaagat ggcaagggca tgcaccattt ctcccacggt atcgggagcg agatagtcga
    11461 attccagcag ctgcgtcttt tcggtgcggc ggtcgcggtc cacgtatttc caggagttgc
    11521 gggccagcgc atacatattg tgcaggaacc aataggccgg catcactacc agctcatcct
    11581 tactgacgtc attactcact aatgcaaacg gaacaggtat atccagctcc gccggataat
    11641 cgcctttagc cagcagcacg aaggaggcga aacgcgaatt atgcttcaga cttacacaaa
    11701 gccccggcca gaaacctcgt ccggcgacca gctcgccatc gggacttcgc gagttatgat
    11761 tggagccgat ggttgctccc gccgccatat tgctctggcc catgacaagc gcggcacaca
    11821 ggaatgaatt attatggtgt tgttcgtgcg cgggaaagat cagcgaattc agtacctcgc
    11881 aacaggagat ggtcgcattg tttcccagat aagaattgat caaccgtgct ccatatttga
    11941 gctgcgaatg cgaggccatg acgaaccgta ctgcctttac tccatagaag gcccggcagc
    12001 caaagccgat gatcccattc accatttcgc aaccttcgcc tatctgcgac atggcttgcg
    12061 gcgaggaatt gatggtcaca ttcttcagct tgttggcgcc cttgagatag gcgtcgctgc
    12121 cgatccatac gtccttgatg atcttacagt tctttatgac ggtgcggtcc ccgaccttac
    12181 catagtaacc gcgccgatgg tcgaaagccg atgcggtaaa ttccttgaac ttttcctgca
    12241 acaccttgtc gtcacgatgc cggctccata gccaggcgtc tcccgggagc attccgttaa
    12301 aaggaatgat gctgcggcct ccgttctcgt tacagacctc cagccaaatg cggatggatt
    12361 catcttcccc atctttgagg atgccatttc cgaacttggc atgatcggtg gtcgccattt
    12421 cattggtatt gacgatcatg acctcgttgc cgatgacata atgggacaga taattggtat
    12481 tgtcgataca gacattgtcg cccagatcgc agctgatgat ggtgctgttg taaaggccga
    12541 cgggtctgcg caggttgtgg aattcgagat accagggatc cagcttgccg atgcgcacca
    12601 gaccgaagaa tttgcagttt tggacgagct cgggtgtgaa agcgtccgag acgaggatgt
    12661 cgttccaatt ggctgaagta ttccggttgc gcacgaggac ctctatctca taggccgtca
    12721 gctgccggta ggggatgccg ctcctgttct gcagattccg cagatagtat tcgtctttgt
    12781 ccttcggcag ataacggttt tcgatgaaac cgtatcccag ggaatggatg ggattttttt
    12841 tgatctcgtt catagcttat tccactgtta cgcttttggc caggttcctt ggtttgtcga
    12901 cgtcatagcc ttttgcgacg cctgtataat aggccaggag ctgcaggggg atgacggtga
    12961 gcatcggggc gatgagttca tccgtctcgg ggatcgggat catgtcatcg gccattttgc
    13021 taataatgct atctccctgc gtgatcactg ccacgacctt gccccttctt gcttttatct
    13081 cctgtatatt gctgatgatc ttctcgtggt agctgtcttt ggtggccacg aagatcaccg
    13141 gaagggtttc ctctacgagc gcgatggggc cgtgtttcat ttctgccgcc ggataccctt
    13201 ccgcatggat ataggagatc tccttcagtt ttagcgcgcc ctccagcgcg acggcaaagt
    13261 tatatccgcg gcctagatac agcgcgtctt tggcgtcctt gtatttttca gccagctgtt
    13321 tgatggcggg gctaagcttg agaacttcct tcgccttttc ggggatctca tgcagctcat
    13381 tgagcagctg catatatcgg gtcggctcga tcgtgccttt ttcgtgcgcg atctttagcg
    13441 cgatcatggt caaaacggca agctgagcgg taaaagcctt tgtcgaagcg acgccgatct
    13501 ccggtccggc atgggtatag gcgccggcat tggatatacg ggcgatggat gagcctacca
    13561 cattgacgac tcccagaatg aatgccccgt tctcttttgc tttttcgatg gcgacgatgg
    13621 tgtcggccgt ctcaccgctt tgcgaaatgg cgatgatcac gtcgccggga tgaatgacgg
    13681 gattgcgata gcggaattcg gaggcatatt ctacttcgac ggggatacgg catagctctt
    13741 cgaaaacata ttcggccacc agccccgcat gccagctcgt accgcaggcg atcatgatga
    13801 tgcggttagc atttttcagc ttgtccatat tctgctggat acccgccatg gtgatcgtcc
    13861 cctttgccgc atcgagacgg ccgcgaagac agtcgaagat agtgtcgggt tgttcgaata
    13921 tttccttgat catgaaatgg tcgtatcctc ctttttcaat gctcgccagc tcgaggtcca
    13981 gcttctggat atagggagtg atcctttcat tgccgaggtt tttcaatacc agctcatcgg
    14041 ctttgaggat cgccagctca taatcgttga catagacgac ttctttggta tattctatga
    14101 tgggcgaagc gtcggaggcc agaaaatgct cgcccttgcc gatgccgatg accagcgggc
    14161 tgcctttccg tgcggcgatc accgtatccg gagcatcttt gtccagcagc actatgacat
    14221 aggcgcctac cacccgtttc aacgcaatac gcactgcttc ctctaaaccg caatcgttat
    14281 tacgctggat atcttctata aaattgagaa ggacttcggt gtcggtgtcg ctgctgaatt
    14341 tgtatccttt ccggatcagc tcgttcttta gttgtgtgaa gttctcgatg atgccgttgt
    14401 gtatcatggc cagtcttcca ctggccgagc tgtgcggatg tgcgttgcgg tcgttgggtt
    14461 caccgtgcgt ggcccaccgg gtatggccca tgccggcatg ggcatgcaga tcgctgccca
    14521 cgaggctgtc ttccagatcg gctacctttc ccttcttttt gtatacccta agattattat
    14581 tcagcaatgc aacccccgaa ctgtcatatc cgcggtattc cagacgcttc aatcctttca
    14641 tgatgatcgg gtaagcttct ctgggtccga tataagcgac gataccgcac ataaggaaaa
    14701 atttagctga gcaatattat tgaataattg tcaaataaaa acgaaagggc ttcctcatcg
    14761 aggaagccct aacaacgtaa attttacact ttaattgcag actactagta gttcgcgggg
    14821 ctcggtttcc aatacccgtc gatccatatc cagccgttgt tcgtgtattt ccagtgtgct
    14881 ttcacccaga tcttgtcgcc cgcgttgccg ctgttcgaat aggacgtcgg cgcgttctga
    14941 ttggtgcccg ggacgttttg taaagaatca ggattgggat aattataagg attgttgtaa
    15001 tagttattgg gcgccgtgtt gttctgtgcc ggcgggggcg gtggcggctg gatgctctgc
    15061 ggaggagctt gttgatagcc ggaaccatcg tcatagggat ccgcgggctc atcataaccc
    15121 gggtcgtaat aggagggagg atatgaataa gaaggatacc cgtaataata ggggtaatag
    15181 ggatagccat aagagtaggg ccagccatag tagccgagac cgaatcctac gccccatccc
    15241 cagccaccat aataatgacc gccataataa cgtccgccag aataatagcg gccgccggaa
    15301 tagtaaccgc cgccatggcc gttatagccg cctctaccgg taacatagcc cctgccggtt
    15361 ccgatataac cccgggcgcc gccgccataa cgcgccccgc caccatatcc tgaggaaacg
    15421 cgtgcgccac cggagaatcc acgtgagccg ccggagaatc cacgtgagcc accgccaaag
    15481 tatccgcggg aaccgccccc gacaaatccg cgggagccac caccggagaa tccgcgggct
    15541 ccgcctccgg aaaaaccacg agctccgccg cctccggaga agctatgacc tccgccaccg
    15601 aagtggccgc cgccgcctcc gtgtccgccg ccgcgctggg catagccggt catgcccgcg
    15661 agggtaagga gcatgatcgc cattacgagt ttaggtctca taaaatacat tttagtaaga
    15721 atagtttacg gtatcccggg ttagaccagc cggggggcag atggtttaat ttacgaaaaa
    15781 tttaatatag acccggggcc gttcgggtca tattacaata aaatgccccg catgatcagg
    15841 aaggcaacgg aaaaatagat caccaggccg gtgacatcga ctaaggtggc tacgaagggg
    15901 gccgaggagg tggccgggtc ggcgccgaat ttcttcagta cgatgggcag catgctacct
    15961 gcgatggtcc cccacatgac gacaccaatc agtgcgaccc cgatggtaag ggcgatcagt
    16021 acgccatgct cgccgtaggt gtggaagatc tggttccaaa ggatgacccg ggtgaaaccg
    16081 atgaccccga gcaccgcccc cagcaggaga cccgagagaa tttcgcgtct catcacccgc
    16141 caccagtcca ggatggtcaa ttctcccagc gccatggcct ggatgatcag tgtcgatgcc
    16201 tgcgagccac tattgccccc gctggagatg atcaggggta caaacagcgc caggacgacg
    16261 gccttcgcga tctcatcctc gaaataggcc atggctgttg ccgtcagcat ttcactgagg
    16321 aaaagcacca ccagccagac gatccttttc cggaacagct tcagcagcgg catttcgaga
    16381 tagggctcat cgagggcttc cgtaccgccg atcttttgta tgtcttcgga gaattcttcc
    16441 tgtgtgatcc acaggacgtc gtcgatggtg acgatcccca gcaggatgtt gttgttgtcg
    16501 agcacgggca gggccacgcg gttgttcatc ttgaacgcct cgctggcggt ctcctgcgca
    16561 tcattgacac tcagtgcgat atagcggttg tcgatgacgt cctcgatctt tttttccggg
    16621 ttggccagga tgatctcgcg gatcctgatg tcatcgataa aatagccttt gtcatcgacc
    16681 acatagatga cgtcgat
//
LOCUS       DASYSV010000566         4407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_886097, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000566 DASYSV010000000
VERSION     DASYSV010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886097"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VGQ51_17670"
     CDS             complement(<1..402)
                     /locus_tag="VGQ51_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEV9038471.1"
                     /translation="MLQKTPSQRLYSLDALRGFDMFWIAGGEEIFHVLAKITGHPFWQ
                     ALSDQFEHPAWNGFHAYDLIFPLFLFIAGVATPYSVGRAREKGDSRKKLLMRVIRRGL
                     ILVLLGVIYNNGLKLHPLSEIRFCSVLGRIGL"
     gene            445..1359
                     /locus_tag="VGQ51_17675"
     CDS             445..1359
                     /locus_tag="VGQ51_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV9038472.1"
                     /translation="MQRKEFLSRSVLAGAAMVSPPLVSRTIASAASDGAPFHLNYGIH
                     DGMFRNLGGPAFTDQIRFAHAQGFRAIEDNGMMDRPADEQTRIGDTLRQLGMTMGVFV
                     LNFDHWPVSTSLCSGDPVWLERFLRACHEAVDVAARCGGKWITVVPGNYDRKLAMGYQ
                     TTHVIHALRKACEILEPHGLIMVLEALSDTPELFLRHPDQTVMICEAVNNPSCKYLFD
                     MYHMQRNEGDLIPNIDRAWEHIAYFQIGDNPGRKEPGTGEMNYHNIFKHIHAKGYTGV
                     LGMEHGMAGTGAEGEKALIKAYRDADDF"
     gene            1423..2256
                     /locus_tag="VGQ51_17680"
     CDS             1423..2256
                     /locus_tag="VGQ51_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9038473.1"
                     /translation="MVSIQNLYFSYGRRKVFDSLNLELKPGTIYGLLGRNGTGKSTLL
                     RNIGGFLFPQQGSIDAFGYTPGKRQPSFLERVFLLPEDFQLPPIGLQRWIKHMAPFYS
                     LFDKPQFERYIGEFNVPTDARLNEMSYGQQKKVLISFALATNAPLLLLDEPTNGLDIV
                     SKSQFRKVIAGAIDEKRSILISTHQVRDLEQLIDRIMIIDEGRIVFDQDLERIASKLL
                     FKFSFDPEEAARAMYRESSLKGNALILRNTDQEDSRPDLEMLYKAITLDPKTLTSIFH
                     P"
     gene            2253..3104
                     /locus_tag="VGQ51_17685"
     CDS             2253..3104
                     /locus_tag="VGQ51_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038474.1"
                     /translation="MKPRSELFNWSRWCLLVSKHWDENRKRYLLALLAIAGLLIVWFS
                     FILVMDKYTPMNVIYQYMTYFVGLYITGSLYASTLFAELSSKRDGVGFLALPASQLEK
                     LLCALLFGILLFFVVYTIIFYAVDIPLVRIANRIIASSPRNYPNTTIRILAMPVYNVF
                     TGAYGPGFEKEMHLFLFAFFSVQSAFLLGSVYFTRYSFIRMVVVLAVGLLGIMTVQAR
                     VILPLLPSGWYNGWTGWFSRDAAGNTDKLVALPESAENAVILLVQCAPPVLLWIVTWY
                     RLKEKQV"
     gene            3104..3475
                     /locus_tag="VGQ51_17690"
     CDS             3104..3475
                     /locus_tag="VGQ51_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV9038475.1"
                     /translation="MQFRESQPIYVQIADFVCEKILLKEWLPGERIPSVRELAVQLEV
                     NPNTVMRTYEFLQQESILFNQRGIGLFTAVDAVAQATRYRKTQFIEKDLPGVFRNLFL
                     LGMDPAELSPRYQHFKKQMKP"
     gene            3472..4281
                     /locus_tag="VGQ51_17695"
     CDS             3472..4281
                     /locus_tag="VGQ51_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038476.1"
                     /translation="MTMRLRKTSDKILVAMVAGAAVLTCCIHLTLYARYRHGDILTVH
                     QLLKTRFVQYHPHRPVVISLVGTIWVNFYPCDSFYIELPKQKDPADKPWMTGQVGPEG
                     NLPMPQYRESGDTLVITGDFAGTLHRPFVDESYSNHLPQVNIYGRDFREIRMLNGQLV
                     LGGSDSMTDAPSIRLTATNSTVEVADYDESTPTPRPKAFFDSLDIHLVNSYMMLNRSA
                     SIRSAGIDLDGNSTLNDRWSTVTRMGITGTDSSHIELAGNNLKHATIDIHH"
BASE COUNT         1019 a         1282 c         1106 g         1000 t
ORIGIN      
        1 cagaccgatc cttcccagga cgctgcagaa ccggatctcg gacagcggat gaagcttgag
       61 cccgttgttg tagatgacgc ccagcaatac gaggatcaga ccgcgccgta tcacccgcat
      121 cagcagcttt ttcctggagt cccctttctc ccgggcgcga ccgacggaat agggcgtcgc
      181 caccccggcg atgaacaaaa agagcgggaa gatcagatca taggcatgaa agccgttcca
      241 ggcaggatgc tcgaactgat cggataatgc ctgccagaac gggtggccgg ttattttggc
      301 aaggacatgg aagatctctt cgccgccggc gatccagaac atatcgaatc cgcgcagggc
      361 gtcaagcgaa tacaatcgtt gtgagggagt cttttggagc atagcgccat gaatataggg
      421 aaattatttc ctacatttat aaccatgcag cgaaaagaat tcttatccag gtcggttttg
      481 gcgggcgccg ccatggtctc gccgccgctg gtcagccgga cgatcgcttc ggctgcgtcc
      541 gacggggcac ctttccacct caactatggc attcatgacg gtatgttccg caatctcgga
      601 ggaccggcat tcaccgatca gatccgtttc gcacacgcgc agggttttcg ggccatcgag
      661 gacaacggca tgatggaccg cccggccgat gagcagacgc gtatcggcga tacccttcga
      721 caactcggga tgaccatggg cgtattcgtg ctgaatttcg atcactggcc ggtaagcacg
      781 tccctctgct cgggcgatcc cgtctggctg gaaagattcc tgcgtgcctg tcacgaagcc
      841 gtcgacgtag ccgcccgttg cggcggcaaa tggatcacag tggtcccggg caactacgac
      901 aggaagctgg ccatgggtta tcaaacgacg catgtcatcc atgcgttgcg aaaggcttgc
      961 gagatcctcg aaccgcacgg tctgatcatg gtcctcgaag cgctgagcga tacgccggaa
     1021 ctttttctgc gacatcccga tcagacggtg atgatctgcg aggcggtgaa caacccctcc
     1081 tgtaaatatc ttttcgacat gtaccacatg caacgcaacg agggtgacct gatacccaat
     1141 atcgaccgcg catgggagca tatcgcctat ttccagatcg gtgataatcc cggccgcaag
     1201 gaaccgggca ccggggagat gaactatcac aatatcttca aacatatcca cgcaaaaggg
     1261 tacaccggcg tcctcggtat ggaacacggg atggcaggca ccggcgccga aggagagaaa
     1321 gccctgatca aggcctatcg cgatgcggac gatttctaaa aaaatttgca gatcccgaaa
     1381 tttcatcgca tcttagtgta ctatttcact agtacaccaa gtatggtcag cattcaaaac
     1441 ctatatttct cctatggccg ccgcaaggtc ttcgacagtc tcaaccttga actaaaaccc
     1501 ggcaccatct atggtctgct cggccgcaac ggcacgggaa agtccaccct tctgcgcaat
     1561 atcggcggtt ttcttttccc tcagcaaggc agcatcgacg ccttcggtta cactccggga
     1621 aaaagacaac cttcctttct cgaaagagta tttctccttc cggaggattt ccagctgccg
     1681 cccatcggtt tacagcggtg gataaaacat atggcgccgt tttattcctt gttcgacaag
     1741 ccccaattcg aacgctatat cggggaattc aacgttccga ctgacgccag gctcaacgag
     1801 atgagctatg gacagcaaaa aaaagtcctg atcagttttg cccttgccac caacgccccg
     1861 ctgctattac tggacgaacc taccaacggt ctcgatatcg tgagcaaaag ccagttccgc
     1921 aaggtgatcg ccggagccat cgacgaaaaa cggtcgatcc tcatcagcac ccaccaggtc
     1981 agggacctcg aacagctcat cgaccggatc atgatcatcg atgaaggccg catcgtcttc
     2041 gaccaggacc tggaacgcat cgcttcgaaa ctgctcttta aattttcttt cgatcccgaa
     2101 gaggcggccc gcgcaatgta tcgtgaatca tcgctcaaag gaaatgcgct gatccttcgc
     2161 aatacggatc aggaagacag ccgccccgat ctggagatgc tgtacaaggc catcacgctc
     2221 gatcccaaaa ccctcacctc tatctttcat ccatgaaacc aaggtccgaa ctctttaact
     2281 ggtcccgatg gtgcctgctg gtctccaaac actgggatga gaaccggaaa cggtatctgc
     2341 tggctctcct cgcgattgcc ggcctgctga tcgtctggtt ctcctttata ctggtgatgg
     2401 ataagtatac gccgatgaat gtcatttacc agtatatgac ctatttcgtc ggtctctata
     2461 taaccggcag cctttatgcc agcactcttt tcgcagagct gagctcgaaa agagacggcg
     2521 tcggtttcct cgcgctaccc gcctctcagc tggaaaagct gctttgcgcg ctgctgttcg
     2581 gcatcctgct ctttttcgtc gtctacacga tcatttttta cgcggtggat atcccattgg
     2641 tcaggatcgc taaccggatc attgcatcct cgccccgcaa ctatccgaac accaccattc
     2701 ggattctcgc gatgcctgtt tacaatgtct ttacgggtgc ctatggcccc ggttttgaaa
     2761 aagaaatgca tcttttcctg tttgcctttt tcagcgtcca atccgccttc ctccttgggt
     2821 cggtctattt tacccgctat tccttcatca ggatggtagt cgtattggcg gtaggactcc
     2881 tcggcatcat gacggtccag gccagggtca tcctgccgct gctgccgtca ggatggtaca
     2941 atggatggac aggctggttt tcccgcgatg cggcgggaaa tacggataag ctggttgcgt
     3001 tgccggagtc tgccgagaac gccgttatcc tgctggttca gtgcgcaccg ccggtcctcc
     3061 tttggatcgt cacctggtat cgtctcaaag aaaagcaagt ataatgcagt tcagagaatc
     3121 acaacccata tacgtgcaga tcgccgattt cgtgtgcgag aagatcctgc tgaaggaatg
     3181 gctccccggc gagcggatcc cttccgtccg ggaattggcg gtccagctgg aagtgaaccc
     3241 gaataccgtg atgcgtacct atgagttcct gcaacaggaa tcgatcctct tcaaccaacg
     3301 gggtatcggt ctttttaccg ccgtcgacgc agtggcgcag gccacccgct atcgcaagac
     3361 gcaattcatc gaaaaggacc tcccgggcgt atttcgcaat ctcttcctgt taggcatgga
     3421 cccggccgag ctgagcccgc gataccagca tttcaaaaaa caaatgaaac catgacgatg
     3481 cgactacgta agacaagcga caaaatatta gtggcgatgg tagccggggc ggcagtactc
     3541 acctgttgta tccacctgac tctttatgcc cgataccggc atggggatat cctcaccgtg
     3601 catcagctgc tgaaaacaag attcgtccaa taccatccgc accggcctgt cgtaatttcg
     3661 ctcgtcggaa ccatctgggt gaatttttat ccttgcgatt cgttctatat cgagctgccc
     3721 aaacagaaag atcccgccga caaaccctgg atgaccggcc aggtaggtcc cgaaggaaac
     3781 ctgccgatgc cgcaataccg ggagtcgggt gacaccctgg tgatcacggg cgattttgcc
     3841 gggacgctcc atcgtccttt tgtcgacgag tcctacagca accatcttcc ccaggtgaat
     3901 atctatggcc gtgatttcag ggaaattcgc atgctgaacg gacagctggt ccttggggga
     3961 tcggatagca tgaccgacgc tccttccatc cgcctcaccg caaccaattc caccgtcgag
     4021 gtagcggatt atgatgagtc tacccccact ccccgcccta aagcgttctt cgacagcctg
     4081 gacattcacc tcgtgaatag ctatatgatg ctcaatcgtt cggcctccat ccggtcggct
     4141 ggcatcgatc tcgacggaaa ctcgacgctc aacgatcgct ggtccaccgt aacccgaatg
     4201 ggaattaccg gcaccgatag ctcgcatatc gagcttgcgg gtaacaatct gaaacacgcc
     4261 actatcgaca tacatcacta aacgtctctc ctatgcaact cgtcatcgat cagctttcca
     4321 agacctactc caacggcgtt tgtgccctga acaatgtatc gctaactctg ggcaacggca
     4381 tgttcggtct tctgggccct aacggcg
//
LOCUS       DASYSV010000567         9672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2547327, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000567 DASYSV010000000
VERSION     DASYSV010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2547327"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VGQ51_17700"
     CDS             <1..1379
                     /locus_tag="VGQ51_17700"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEV9038477.1"
                     /translation="YDNNIYTWTNGNFDPYLVMTNVNQMQLEVTSRGGTRVNPYEAVG
                     RFIIAYYYYNLTSLYGDVPEAQALEAPKNSNPAYTPQEQVFKYVLDQLDSANTDMAAL
                     IAQNDNSLSTTQDIYYHGDLTLWQKAVNSFKLRVLVALSPKASDATLNVPAQFSAILG
                     NSSKYPVFTSQGDDLEFVYNPGGGNTYSTYPFNPSNFGSIAARFNMAKTYVDAVVGLD
                     DPRVFITCEPAWTLVGSDPNPAQYKYFAGASTGESVASMYNNANTGIYSFINRKRYYS
                     NFTGEPDVLLGYKELCFNIAEGITRGWASGNAETWYKKGITESMAFYGIDVSKTGFDA
                     YFLPPGANSVTQVAPYPFTFNFTTWYAQPSVTLSSTAATAINQIVLQKYIASFENSGY
                     ESYCNWRRTGTPTFLGGSGVGNNGVVPIRWAYPVSEQAVNASNWNAAVKNQGWNADDL
                     NQKMWLLK"
     gene            1451..2032
                     /locus_tag="VGQ51_17705"
     CDS             1451..2032
                     /locus_tag="VGQ51_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV9038478.1"
                     /translation="MTAEQAEKITDEIMSLYEGYGGAEYAGEKVSQLQHMVQAAQLAE
                     EQGYDEEVVLAAFLHDIGHISEAANGNNAMDGFGIKDHEELGAEFLREKGFSKKIARL
                     VESHVEAKRYLTVKDPGYYDQLSEASKQTLEFQGGPMTREEAEAFEQYPLFPLIIQMR
                     KWDEQAKIENKPLPDLQHYRQMILHHLRTAAPR"
     gene            2069..2761
                     /locus_tag="VGQ51_17710"
     CDS             2069..2761
                     /locus_tag="VGQ51_17710"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEV9038479.1"
                     /translation="MSSIQLVVFDIAGTTVRDNGQVAEAFISAFGDFGFEMPLAEVKK
                     VMGFRKIDSITLLLEKFAPEYMDDDILIDRIHTRFIDKMIDFYRNDEDLAPLPHAEEV
                     FASLHSKGIRIALNTGFTRSITDTILHRLRWDNRHPYIDKVICSDEVTNGRPFPDMIQ
                     TLIADLGIASPDKVMKIGDTEVDVEEGRNANCGTVVSVTTGAYSRQQLEGYQPDFIID
                     DLRELLPIIEKA"
     gene            2772..4097
                     /locus_tag="VGQ51_17715"
     CDS             2772..4097
                     /locus_tag="VGQ51_17715"
                     /inference="COORDINATES: protein motif:HMM:NF039829.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5690 family protein"
                     /protein_id="HEV9038480.1"
                     /translation="MQSSPVTPSTAESALPVALTLKARLARAPGWVFILYLSLSSFTV
                     YACMYGFRKPYTAASYEGLSLLGISYKVVLVIVQTIGYMLSKFYGIRFIAGMRPERRG
                     RYIVVLIGISWVALLLFAMVPAPYNFVFMFINGLPLGMIWGLTFGFVEGRRTTELIGA
                     VCSTSFIFASGIAKTVGKTIVLRGVSETWMPFLAGGVFLIPLLICTWLLNQTPPPTPA
                     DVAQQTIRLPMTKEQRRGFLRVFGFALVPIVFAYIMLTIMRDFTEDFANELWTATGYG
                     HDAGVFANTSTIVGLIVLAVLGGMFLIRNNYRAFQLNNWIIIFGFVLAAAATAAFRFH
                     LISPVAWIITASAGLYLGYVPYNCFYFERMLAAYRISGNVGFLIIYIADAFGYLGTVV
                     VLLAREFVRFHYTWVDFFTGMFYFSAFAGIILVLVGSTLFAKIYRNNNS"
     gene            4107..5237
                     /locus_tag="VGQ51_17720"
     CDS             4107..5237
                     /locus_tag="VGQ51_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03364 family FAD-dependent oxidoreductase"
                     /protein_id="HEV9038481.1"
                     /translation="MASRSAIVIGAGIVGLATARALAIRGFTVKVFERSEKAVGASIR
                     NFGMIWPIGQPIGKMYDRAVRSRDVWKEIASRADFWHEPAGSLHLAYHPDEWVVLQEL
                     YHIFSKEGRPVRLLDKDAVAATSSAVISKGLLGGLYSSDEVIVDPREAIATLPGWLAE
                     QFPITFHWGKCVSYISDQIVYIGNDEEHEADLIFICNGADFETLYPEQFAQQPVTRCK
                     LQMMRLVSQPEDWRIGPALCGGLSLIHYNSFKAAPSLPVLRQRYAGEMPDYLEWGIHV
                     MVAQNGRGELTVGDSHEYGLTHDPFDKDFINRKILDYLRCFACFRDWTVTETWNGVYA
                     KLTDGEADLFFSPEPYVYVINGVGGAGMTLSFGLAEELVAGI"
     gene            5346..5984
                     /locus_tag="VGQ51_17725"
     CDS             5346..5984
                     /locus_tag="VGQ51_17725"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV9038482.1"
                     /translation="MKTLRAIIIGNRLFFILYAVFFAIGLIFLLNVGKAAAFVDLNPY
                     HRSALDAVFAWITFLGDGTFSVIVIVIFLLKRRWSGATQITAAFLLSALLAQILKRVF
                     SMPRPMQFFPAGHYPYFIKGVTHIGFASFPSGHSTTIFALATLLAIFTKDQRLKAACL
                     LGAVLVGYSRIYLGQHFLGDVLMGSCLGILTAVGIHWLFVQKLQTLPLFARG"
     gene            6079..6828
                     /locus_tag="VGQ51_17730"
     CDS             6079..6828
                     /locus_tag="VGQ51_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV9038483.1"
                     /translation="MIEGKKLVIVLPAYNAELTLRKTYAEIPFGIVDEVVLVDDNSSD
                     RTVAVAHELGIHHIIKHQQNRGYGGNQKTCYNKALELGADIVIMLHPDYQYTPKLIPS
                     MAWLIANDVYQCVLGARTLGKGALHGGMPLYKYVFNRALTFFENVLLRQKLAEYHTGY
                     RAFSREILQRINFEVNSEDFVFDNQMLSQIIYLGYEIAEITCPTRYFEEASSINFRRS
                     VVYGLGVLQVSVVHRLCKWGVMKREMYKPLK"
     gene            6829..8541
                     /locus_tag="VGQ51_17735"
     CDS             6829..8541
                     /locus_tag="VGQ51_17735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV9038484.1"
                     /translation="MRLNKSVLVLLIAVAAALLFIPFLGHVHLFDWDEINFAEASREM
                     LKLHDYTRVYIDYKPFWEKPPMFFWMQSLAMTVFGISEFAARFPNAVCGMVTLIAVFL
                     CGSRIYDRKFGLLWALAYGGSLFPNMYFKSGIIDPWFNLFTFLSLYNFILYHWKRNKF
                     DKANLGRSQWYYVIWSGIFMGLAILMKGQVALMIFLLTLGVYLIYNRFRIYFSWWHAL
                     LFLVVAFFVTFSWYGYETIKRGPWFIEQFLKYQWRLFRTHDADQAGFFGYHYIVLLVG
                     CFPASILAIPSFFRAKYNGRYDRDFKKWMVLLFWVVTVLFTIVQSRIIHYSAMAWFSV
                     TFLGAYSIYKWDRKELNYKKYVGIGLGILGGLISLLILGVAIIGLNVHRLAPYVQDTF
                     ARGNMEADVHWTGFEGVIGLLMIVALVLGIRYLSRRNFTAAARSLFIGTAVVVFFASA
                     IIVPKVERYSQGAAIDFFIARQGENCYVHTLGYKSYGDLFYTHKEQPTNPNAWSEQWL
                     LTGPIDKPVYFVTKVDRVQHWEDAAKYPDLRELYRKNGFVFLKRDVPVAVTPPDSAAS
                     SPAK"
     gene            complement(8557..>9672)
                     /locus_tag="VGQ51_17740"
     CDS             complement(8557..>9672)
                     /locus_tag="VGQ51_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HEV9038485.1"
                     /translation="GTTSTKLAGINDLILAIQKANRYFLEQTQRQVNTALTLRNWVIG
                     YYIVMYEQKGEERAEYGERLLKNLAERIKKTEIRGLGFSILKLCRQFFWAYPQIGQSV
                     TDQIQLLDYHPNEIIQTASKQFVGASPNPTDLLLNRLSFTHFAELVKADGPVKRIFYE
                     TETIVNNWSVRELQRAMDSMLFERTGLSKDKEAVLEKHRNGTGMKPEDIFRSPHLLDF
                     LGLQEKPEYVESDLEQAIIDHLQNFLLEMGRGFCFEARQKRITFDNTHYRIDLVFYHR
                     LLRCHVLIDLKIGRFTPADAGQMYVYLNYFKENEAGEGDNPPIGIILCTDKSETLVKY
                     ATAGLDQHLFVSTYMTRLPSEKELEEVIREERVRRAA"
BASE COUNT         2114 a         2743 c         2373 g         2442 t
ORIGIN      
        1 actacgataa caacatctac acctggacca acggtaactt cgatccctac ctggtcatga
       61 ccaatgtcaa tcagatgcag ctggaagtca cctccagggg cggcaccagg gtaaacccct
      121 atgaagccgt gggcaggttt atcatcgcct attattatta caacctcacc tctctctacg
      181 gtgatgtccc cgaggcgcag gcgttggagg cgccgaagaa ttccaatcct gcgtatacgc
      241 cgcaggaaca ggtattcaag tatgtgctcg atcagctgga ttcggccaat accgatatgg
      301 ccgcgcttat cgcgcagaac gacaattccc tgtccacgac gcaggacatc tattatcacg
      361 gcgatctcac gctttggcaa aaggcggtga attctttcaa actgcgggta ttggtagcgc
      421 ttagtcccaa ggcttcggac gccacgctga acgtccccgc ccagttctcc gctatcctgg
      481 gcaattcatc caaatatccc gtctttacga gccagggcga tgatctcgaa ttcgtctaca
      541 accccggcgg cggcaatact tacagcacct atccgttcaa cccgagcaac ttcggctcca
      601 ttgccgcccg cttcaacatg gccaagacct atgtggatgc cgtcgtcggt ctcgacgacc
      661 caagggtatt catcacctgc gaaccggcat ggacgctggt aggcagtgat cccaaccccg
      721 cgcaatacaa gtatttcgcc ggggccagca ccggcgagtc ggtggcttcg atgtacaata
      781 acgccaacac tggaatatat tccttcatca accggaaaag gtactattcc aacttcaccg
      841 gtgaaccgga cgtgctgctc ggttacaaag agctttgctt caatatcgcg gagggtatca
      901 cccgtgggtg ggcctccggc aacgcggaga cctggtataa aaagggtatc accgaatcca
      961 tggcttttta cggcatagac gtcagcaaga ccggctttga cgcctacttt ctgccgcccg
     1021 gcgccaactc ggtgacccag gtggctccgt atccctttac tttcaatttc accacctggt
     1081 atgcccagcc atccgtgacg ctgtcttcaa cagcggctac ggccatcaac cagatcgtac
     1141 tgcagaaata catcgccagc ttcgagaatt cgggctatga atcttattgt aactggcgca
     1201 ggacgggtac tccgaccttc ctgggcggca gtggcgtcgg aaacaatgga gtggtgccta
     1261 tccgttgggc ctatccggtt tccgaacagg ccgtcaatgc gtccaactgg aatgccgcgg
     1321 ttaaaaacca gggatggaac gccgacgatc tcaaccagaa gatgtggttg ttgaaataaa
     1381 ttgttatttg tggcgccgcc gtatggcggc gccactttta aacagtattt ttattccaaa
     1441 accatccctt atgactgctg aacaggccga aaaaattacc gatgagatca tgagccttta
     1501 tgaagggtat ggcggcgccg aatatgcggg cgaaaaggtt tctcagctgc aacacatggt
     1561 acaggctgca caactggccg aggaacaggg ttatgacgaa gaagtggtat tagccgcctt
     1621 cctccacgac atcggacaca tcagcgaagc tgccaacggg aataacgcga tggacggctt
     1681 cggaattaaa gaccatgaag agctgggcgc cgaattcctc cgcgaaaagg ggttctcgaa
     1741 aaaaatagcc cgcctcgtcg agtctcatgt agaggcaaaa aggtatctca cggtcaaaga
     1801 ccccggctat tacgatcagc tctccgaagc cagcaaacaa acgctcgaat tccagggggg
     1861 ccccatgacc cgtgaagaag cggaggcctt cgaacaatat cctctctttc ccctgatcat
     1921 ccagatgcgg aaatgggacg aacaggcgaa gatcgagaat aaaccattgc ccgatctgca
     1981 acactaccgc caaatgatcc tccatcatct tcgcaccgcc gccccccgat gacccgtgac
     2041 ctatcccaac cataaactga atttttttat gagctctatc caactcgtcg tttttgacat
     2101 agcaggcacc accgtccgcg ataatggaca ggtagcggaa gccttcatct ccgcattcgg
     2161 cgatttcggc ttcgaaatgc ctcttgcgga agtaaaaaag gtcatggggt tccggaagat
     2221 cgactccatc acccttctac tggaaaaatt cgctcccgaa tacatggacg atgatatcct
     2281 catcgaccgc atccataccc gattcatcga taagatgatc gatttttatc gcaacgatga
     2341 agatctggcg ccgctgccgc acgccgaaga ggtctttgcc tcgctgcaca gtaaaggcat
     2401 ccgtatagcc cttaacaccg ggttcacccg cagcatcacc gataccatcc tgcacagatt
     2461 gcgctgggat aaccgccacc cgtatatcga taaggtcatc tgcagcgacg aagtgaccaa
     2521 cggccgccct tttccggata tgatccaaac cctgatcgcc gacctgggca tcgcatcccc
     2581 cgacaaggtg atgaagatag gcgataccga agtggacgta gaagaaggtc gcaatgcgaa
     2641 ttgcggtacg gtagtcagcg ttactacagg ggcctatagc cgccagcagc tggagggata
     2701 ccagcccgac tttatcatcg acgatctgcg cgagctgctt cctattatcg aaaaggccta
     2761 aacccgacag catgcaatct tcaccggtca ccccttccac cgcagagtcg gcattgccgg
     2821 tggctctgac gctgaaagca cgtctggcgc gggcgcccgg atgggtcttc atcctgtatt
     2881 tgtctttatc ctcctttacg gtctatgcgt gtatgtacgg ttttcgcaaa ccatacactg
     2941 cggcttccta tgaaggactc tccctgctcg gtatctctta taaggtcgtg ctggtcatcg
     3001 tgcagaccat cggctatatg ctgtccaaat tctatggtat ccgcttcatc gcgggcatgc
     3061 gtcctgagag aagaggtcgc tatattgtag tactgatcgg catctcatgg gtcgctttgc
     3121 tccttttcgc gatggtgccg gcgccataca attttgtatt tatgttcatc aacggtctgc
     3181 cgctgggcat gatctgggga cttacctttg gctttgtcga aggtcggcga accaccgagc
     3241 tgatcggcgc cgtatgctct accagcttca ttttcgcttc gggcatcgcc aagaccgtgg
     3301 gcaaaactat cgttcttcgc ggtgtcagcg agacctggat gcccttcctg gcaggcggcg
     3361 ttttcctcat cccgctgctg atctgcacct ggctcctcaa ccagacgccg ccgcctaccc
     3421 ccgcggacgt cgcccagcaa acgatcaggc ttcccatgac caaagagcaa cgcaggggtt
     3481 tcctgcgggt cttcggcttc gcactcgttc cgatagtttt cgcttatatt atgctcacca
     3541 tcatgcggga ctttacggaa gatttcgcca acgaattgtg gaccgcgacc gggtacggtc
     3601 atgatgccgg tgtctttgcc aataccagca ccatcgtcgg gctcatcgtc ctggccgtgc
     3661 tgggaggcat gttcctcatc cggaacaatt accgggcctt tcagctcaac aactggatca
     3721 tcatcttcgg cttcgtactg gccgccgcgg ctaccgccgc cttccggttc catcttattt
     3781 cacccgtcgc ctggatcatc accgcctcgg ccggtcttta cctgggttat gtaccctata
     3841 actgtttcta tttcgagcgg atgctcgccg cctataggat atcgggtaac gtggggttcc
     3901 ttattattta catcgccgat gcattcggct atctggggac ggtagtcgtc ctgcttgcca
     3961 gggaattcgt ccggtttcac tatacctggg tcgatttttt tacgggtatg ttctatttct
     4021 ccgcgttcgc ggggatcatc ctcgtgctcg tgggctccac gctgtttgca aaaatttaca
     4081 ggaataacaa ttcataaagg cattctatgg catcccgatc agcaatcgtt ataggcgcag
     4141 gtattgtagg attggctacc gcaagggccc tggccatccg gggatttacg gtgaaggtct
     4201 tcgaacgcag cgaaaaggcc gtaggcgcgt cgatccgcaa tttcggcatg atatggccga
     4261 tcgggcagcc catcgggaag atgtatgaca gggccgtgcg aagcagggac gtatggaagg
     4321 agatcgcctc ccgcgcggat ttctggcatg agcccgcggg aagcctgcat ctcgcctatc
     4381 accccgacga atgggtggtc ctccaggaat tgtaccacat cttcagcaaa gaaggacgtc
     4441 ctgtccggct gctcgacaag gatgccgttg ccgctacctc gtcggcggtc atctcaaaag
     4501 gcctgctagg cggcctatat agcagcgacg aggtcattgt cgatccccgc gaagccatcg
     4561 ccaccctgcc cggctggctg gcagaacaat tccccatcac ctttcactgg ggtaaatgcg
     4621 tttcctatat ttccgaccag atcgtctata tcggcaatga cgaagaacac gaagccgatc
     4681 tgatctttat atgcaatggg gcggattttg agaccctgta tcctgaacag tttgcacaac
     4741 aacccgtgac caggtgcaag ctacagatga tgcgcctggt ctcgcaaccg gaggactggc
     4801 gcatcggccc cgccttatgc ggcggattgt ccctcatcca ctataatagc ttcaaagccg
     4861 caccttcgct cccggtgctg cgccaacgtt atgccgggga gatgcccgac tatctcgaat
     4921 ggggcatcca tgtaatggtc gctcagaacg gccggggcga gctcacggtc ggcgattcgc
     4981 atgaatatgg tcttacccac gatccattcg ataaggactt cataaaccgg aagatcctcg
     5041 actatctccg ctgttttgcc tgcttccgcg attggaccgt gaccgaaacc tggaacggcg
     5101 tctatgcgaa gctcaccgat ggagaagccg atctcttctt ttcccccgaa ccttatgtct
     5161 acgtgatcaa tggggtagga ggggcgggta tgacgctgtc cttcgggttg gcagaagaac
     5221 tcgtggcggg gatttagcgc cctcacgccc cctcgcgcct cctcgcgcgc cctcacgccc
     5281 ttcgcgcgcc cgatccagcc gcggacattc ccgcgccatt tccccggatt ccttaatttc
     5341 gcctgatgaa aacgctgaga gcgattatta tcggcaatcg cctttttttt attttatacg
     5401 ccgtgttttt cgccatcggc ctgatcttcc tcctcaacgt cggaaaagcc gccgcctttg
     5461 tcgatctaaa cccataccac cgatcggccc tggatgcagt tttcgcctgg atcacctttt
     5521 tgggcgacgg caccttcagc gtgatcgtca tcgtcatctt tctgttgaaa cgtcgctggt
     5581 ccggcgcgac ccagatcacc gccgcctttc tgctctcggc cctgctcgcg cagatactca
     5641 aaagggtctt ttcgatgcca agaccaatgc aattcttccc tgcgggccat tatccctatt
     5701 ttattaaagg cgtcacccat atcggtttcg ccagtttccc ctcaggccac tccaccacta
     5761 tcttcgcact tgcgacgctg ctggctattt ttacgaaaga ccagcggctg aaagcggcct
     5821 gtctcctcgg cgccgtactg gtcggctatt cccgcattta tctgggacaa catttcctcg
     5881 gcgatgttct gatggggtcc tgtctcggga tccttacggc tgttgggatc cactggcttt
     5941 tcgttcaaaa actgcagact ttacccctat ttgcgcgtgg atagaccttt ttaccctaaa
     6001 atcaagtagt tttgccctca atttttccgg cggcttaccc aaaaaaggaa gcttatagcc
     6061 tttaaacaaa gagtatacat gatcgaaggc aaaaaactgg tgatcgttct gccggcctat
     6121 aacgccgaac ttacgcttcg caagacctac gctgaaatcc cttttggcat cgtagacgaa
     6181 gtggtgctgg tggacgacaa cagcagcgac cggaccgtag cggtcgccca cgagctgggc
     6241 atccatcata tcatcaagca tcagcagaac cggggttatg gcggcaatca gaaaacctgt
     6301 tacaataagg ctctcgaatt gggcgccgat atcgtgatca tgctccatcc cgattatcaa
     6361 tatactccca aattgatccc gtccatggcc tggctgatcg ccaacgacgt gtatcaatgc
     6421 gtgctgggag cgaggaccct gggtaaaggc gccctccacg gcggaatgcc cctgtacaaa
     6481 tatgtattca accgcgcgct cacgtttttc gaaaacgttc ttttgcgtca gaagctggcc
     6541 gaataccata ccggctaccg ggccttttcg cgcgagatcc tccagcggat caatttcgag
     6601 gtcaattcgg aagatttcgt tttcgataat caaatgctct cccagatcat ttatctcggc
     6661 tatgagattg cggagatcac ctgtccgacc cgctatttcg aagaggcttc gagcatcaat
     6721 ttccggcgca gcgtcgtcta tgggctggga gtgttacaag tctccgttgt ccatcgactc
     6781 tgtaagtggg gagtaatgaa gagagaaatg tataaaccgc tgaaataaat gcgtctcaat
     6841 aaatctgtac ttgttctgct gattgctgta gcggccgcgc tgctcttcat cccattcctg
     6901 ggtcatgttc acctgttcga ctgggatgag atcaattttg ccgaagcctc ccgcgaaatg
     6961 ctgaagctcc atgattacac ccgcgtttat atcgactata aaccattctg ggaaaagccg
     7021 ccgatgttct tctggatgca aagcctggcg atgaccgttt tcggtatctc ggagttcgcc
     7081 gcccgctttc ccaatgccgt ctgcggaatg gttaccctta tcgccgtttt tctttgcggc
     7141 tcgcgcatct atgacaggaa attcggtctg ctctgggcgc tggcctatgg cggatcgctc
     7201 ttccccaata tgtacttcaa atcgggtatc atcgacccct ggttcaatct tttcaccttt
     7261 ctttcgctat acaatttcat cctctatcac tggaaaagga acaagttcga caaggccaat
     7321 cttgggagaa gtcagtggta ttacgtcatc tggtccggca tcttcatggg tctggccatc
     7381 cttatgaagg gccaggtcgc gctcatgatc ttcctgctga ccctgggggt gtatttaatt
     7441 tataaccgct tcaggatcta tttttcctgg tggcatgccc tgctcttcct ggtcgtcgcc
     7501 ttcttcgtga cgttcagctg gtacggatac gagaccatca agcgtggtcc ctggttcatc
     7561 gagcaattcc tgaaatatca atggcgtctc tttcgtacgc acgacgccga tcaggcgggc
     7621 ttcttcggct atcattacat cgtcctgctg gtgggctgtt tccccgcatc catcctggcc
     7681 attccatcct ttttccgggc taaatacaac ggacgctatg atcgcgattt caagaagtgg
     7741 atggtactgc ttttctgggt agtgaccgta ctgttcacga tcgtacaatc acgcatcatc
     7801 cactattcgg cgatggcgtg gttctccgtc acgttcctcg gcgcctactc catttataaa
     7861 tgggatcgca aagagctgaa ttataagaaa tatgtgggca tcgggctggg catcctcggg
     7921 ggacttatct cgctgttgat acttggcgtg gccatcattg gtctgaatgt acatcgcctg
     7981 gcgccctatg tgcaggatac ttttgcccgc ggcaacatgg aggcggacgt ccattggacc
     8041 ggtttcgaag gtgtgatcgg ccttttgatg atcgtcgccc tcgtgctggg gatccgctac
     8101 ctgtcacgcc gtaatttcac tgccgcggcg cggtctcttt ttatcggtac cgccgtagta
     8161 gtcttcttcg cctctgccat catcgtcccg aaagtcgagc gctacagcca gggcgccgcc
     8221 atcgacttct tcatcgcgcg ccagggcgaa aactgctatg tgcatacgct cggctataaa
     8281 agctacggcg acctcttcta tacccataaa gaacaaccca ccaaccccaa tgcctggtcc
     8341 gagcagtggc tcctcaccgg cccgatcgac aagcctgttt atttcgtcac caaggtggat
     8401 cgtgtgcagc attgggaaga tgcggctaaa tatcccgatc tgcgggagct gtaccggaag
     8461 aatggtttcg tgttcctcaa acgcgacgtc ccggtggccg tcactccacc tgactcggct
     8521 gcctcgtcgc ctgcgaaata attctgattg ttttcattat gctgcgcgcc tgacccgttc
     8581 ttcgcgtatc acttcttcca attctttctc actgggtagg cgggtcatat atgtggatac
     8641 gaaaagatgc tgatccaacc ccgctgttgc atatttaacc agcgtctcgc ttttgtccgt
     8701 gcaaagaata atgcctatcg gcggattgtc tccttcacct gcttcatttt ccttaaagta
     8761 gttgaggtac acgtacattt gaccggcatc agccggggtg aaccgtccaa tctttagatc
     8821 gatcaggaca tgacatctta acaaccggtg atagaagacc agatcgatcc ggtaatgcgt
     8881 attatcgaat gtgatccgtt tttgtcttgc ttcaaaacag aagcctcttc ccatctccag
     8941 cagaaagttt tggagatgat cgataatggc ctgttccaga tcgctctcta catattcggg
     9001 tttttcctgc agccctaaaa aatccaaaag gtgcggactt cgaaaaatgt cctctggttt
     9061 catcccggta ccgtttctat gcttttcaag aaccgcttct ttgtccttgc ttaagccggt
     9121 gcgctcaaat aacatgctgt ccatggctcg ctgcagctcg cgtacggacc aattattaac
     9181 aattgtttcc gtctcataaa aaatgcgctt tacaggacca tctgctttta ccaattcagc
     9241 gaaatgagta aaagagagcc ggttgagtaa caaatccgtt ggatttgggg aagcccccac
     9301 gaattgtttg gacgctgtct ggataatttc gtttggatga taatcaagta attgtatttg
     9361 gtcagtcact gactggccaa tttgagggta ggcccaaaag aattgccggc atagtttaag
     9421 tatgctgaag cccaaccccc taatctccgt cttttttata cgttccgcga ggttttttag
     9481 tagtctttct ccatattctg ccctttcctc tcccttttgc tcgtacatga cgatataata
     9541 tccgatgacc caattgcgaa gcgtaagggc tgtattcacc tgtctctgcg tctgctccag
     9601 gaagtatcga ttcgccttct gtatcgccag gatcaaatca ttgatcccag ctagttttgt
     9661 cgatgttgtt cc
//
LOCUS       DASYSV010000568         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_239812, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000568 DASYSV010000000
VERSION     DASYSV010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239812"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VGQ51_17745"
     CDS             complement(<1..367)
                     /locus_tag="VGQ51_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038486.1"
                     /translation="MRRTIIFAVFFLPVLCSGQKHYDFNAVCQQAYHSIVQLKLEEGR
                     RLLDEEKRRDPGNLIPYFLDNYIDFFQLFFNEDATQYAAWKDRRDQRLDLMSQGPEGS
                     PWNLFTRSLIHFQWAAIQIK"
     gene            complement(375..1130)
                     /locus_tag="VGQ51_17750"
     CDS             complement(375..1130)
                     /locus_tag="VGQ51_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEV9038487.1"
                     /translation="MSSVKEFSVEEKLTSLVTLQKVESKLDEYKILKGELPMEVSDLE
                     DEITGLHSRQTRVEEEINGIQEFINQKKEAIKEADALIKKYDKQSQNVKNSREFEAIN
                     KEIEMQQLEVKLAEKHIKDAGEEIAEKVLLLEKAKKNIGTKEAVLKNKKDELDKIIAT
                     TEKEEKHFTKLSTEAREKVESRLLHSYDRIRGNYRNGLAVVPVLRDACGGCFNAIPPQ
                     RQSEIRQRKKIIVCENCGRILVDNELNDSIEVK"
     gene            complement(1118..2227)
                     /locus_tag="VGQ51_17755"
     CDS             complement(1118..2227)
                     /locus_tag="VGQ51_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV9038488.1"
                     /translation="MNISAVVDFLETLAPPAYQEHYDNAGLLTGSRSWECTGALTTLD
                     VTEQVVEEAAARGCNLIVAHHPIIFKGLKRLTGNGYVARTVISAIKRDTAIYAIHTNL
                     DHMVAGGVNGRIAAQLGLQGGAVLLPKEGVLQKLLCFVPVDHVESVRAAMFAAGAGHI
                     GGYSECSFSGEGMGTFLGGEGTDPFVGERGIRHQEKEMRLEVVVPAHLSASVVAAMKA
                     AHPYEEVAYDLVSLANTHPGVGAGLFGDLPVAMEERAFLEHLGAVFGLAVIRHTPLTG
                     RPVKRVAVCGGAGSFLISSALGVGADFFITADVKYHEFFDADGRLVLADIGHFESEQF
                     TMDLLFEVLQEKFRNFAVLKSETKTNPVKYYLCQA"
     gene            complement(2236..>3175)
                     /locus_tag="VGQ51_17760"
     CDS             complement(2236..>3175)
                     /locus_tag="VGQ51_17760"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEV9038489.1"
                     /translation="FGVSGLVFYDTAKAYYQFNINRNLSNEAAVTFNTGLLRAVRSAK
                     PLVSSFDGWTAEDSAYLRRNKFVVQELARAKPIEDQKVKTLESVTVRGHVKTDAEKLD
                     ERYASGMFSGGDAYEYDLVNDQLASSYPDIFTYLQGKVPGLQIVAGQGPGGTASLSWR
                     GGRPSLYLNEMQMDVQGLQNTPVSDIAYVKVFRPGSGVGFGGGAGGVIAIYTKKGGDE
                     KRNDANFKGLDRQILIGYSMPKEFYSPNYLVSSPLNEREDLRTTLYWKPYILTDKFTK
                     QVTIEFFNNDISRKLRIVLEGFNEDGKLAHLEQIIQ"
BASE COUNT          713 a         1006 c          697 g          759 t
ORIGIN      
        1 attttatctg tatcgcagcc cactgaaaat ggatgagact ccgcgtaaaa agattccacg
       61 gcgaaccttc gggaccctga ctcatcagat ccagccgctg atcccgtcga tccttccagg
      121 ctgcatactg cgtagcgtcc tcgttgaaga atagttgaaa gaaatcgata tagttatcca
      181 ggaaataggg gatcagatta ccggggtccc tcctcttctc ctcgtcgagc aaccgccgcc
      241 cttcctccag cttcagctgg acgatagaat gataggcttg ctgacaaaca gcattgaaat
      301 catagtgctt ttgcccggaa cacagcacgg gaagaaaaaa gacggcaaaa ataatggtcc
      361 gcctcaccgg ccccttattt gacctcgatg gagtcgttta gctcattgtc caccaggatc
      421 cgtccgcagt tctcgcaaac gatgatcttc ttgcgctggc ggatctcgct ctgacgctga
      481 ggtgggatgg cattgaaaca gccgccgcac gcgtcacgga gcaccggaac aaccgcaaga
      541 ccgttgcgat aatttccgcg gatacgatca taggaatgca atagccggga ttccactttt
      601 tcacgcgctt cggtagacag tttcgtaaaa tgtttttctt ctttttcggt cgtggcgatg
      661 atcttgtcca gctcgtcctt tttattcttc agcacggctt ccttggtgcc gatattcttc
      721 ttcgcctttt ccaaaagcag caccttctcg gcaatctctt caccagcatc cttgatatgc
      781 ttttcggcca gcttcacttc cagctgctgc atctcgatct ccttgttgat cgcttcgaac
      841 tcgcggctgt tctttacatt ctggctttgt ttatcgtatt tcttgatcag cgcatcggcc
      901 tccttgatcg cttccttctt ctgattgatg aattcctgga tcccgttgat ctcttcttca
      961 accctggtct gacgggaatg aagacccgtg atctcgtctt ccagatcgct gacttccatc
     1021 ggcaattcgc ctttcaggat cttgtactcg tctagtttgg attcgacttt ctggagtgtc
     1081 accagcgagg tgagcttttc ctccacggag aattctttta cgcttgacat aagtagtatt
     1141 taaccggatt cgtttttgtc tccgatttaa ggaccgcgaa attacgaaat ttttcctgca
     1201 aaacctcaaa aagcaggtcc atagtgaact gctcgctttc gaaatgcccg atatcggcca
     1261 acaccagccg gccatcggcg tcaaaaaact catgatattt tacatccgcc gtaataaaaa
     1321 aatccgcccc aacccctaaa gcgctggaaa tcaaaaaact accagcaccg ccgcaaacag
     1381 ccacccgctt caccggacgc cctgtcagag gcgtatggcg tatcaccgcc aatccaaaaa
     1441 ctgccccaag atgctccagg aacgcccgct cctccatggc caccggcaaa tccccgaaaa
     1501 gacccgcccc caccccggga tgcgtattgg ctagcgacac caggtcatat gccacttcct
     1561 cataaggatg cgcggccttc atagccgcga caacagaagc gctcaaatgc gcgggcacca
     1621 ccacctccag cctcatctcc ttttcctggt gcctgatccc ccgctcgccc acaaatggat
     1681 ccgtcccttc cccgcctaaa aaagtcccca tcccctcccc cgaaaagctg cactcgctgt
     1741 atccaccgat gtgccctgcc cctgccgcaa acatcgccgc ccgaacggac tccacatgat
     1801 ccaccggcac aaaacacaac agtttctgta acaccccctc cttcggcaac aagacagcac
     1861 ctccctgaag acccagctgc gcggcgatcc gcccgttgac gcctccggca accatatgat
     1921 cgagattggt gtgaatggca tagatcgcgg tatcccgctt gatggcggag atcaccgtcc
     1981 gtgccacata gccgttgccg gtaagtctct tcagcccctt aaagatgatg ggatgatgcg
     2041 ccacaattaa attgcagccc cgggccgccg cttcttccac cacctgctcc gtgacatcca
     2101 gggtggtcag cgctcccgta cattcccagg agcggctccc ggtgagcagt cccgcattgt
     2161 cgtaatgctc ctggtaagcc ggcggggcca gcgtctcaag aaaatcgacc acagcagata
     2221 tattcatgcc acactctatt gtatgatctg ctctaaatgc gcaagcttcc cgtcctcgtt
     2281 gaatccttcc agcacgatcc gcagcttccg cgaaatatcg ttattaaaaa actcgatagt
     2341 cacctgtttg gtgaacttat cggtgagtat atacggcttc cagtacaatg tcgtccgcag
     2401 gtcctcgcgc tcgttcagcg gcgaactcac gagatagttc ggcgaataga attctttcgg
     2461 catcgaatat ccgatcagga tctgccggtc cagccccttg aagttcgcgt cattgcgttt
     2521 ctcatcccct ccctttttcg tgtaaatcgc gatcacgccg ccggccccgc cgccgaaccc
     2581 tacgcccgac cccggacgaa agaccttcac ataggcaata tccgataccg gtgtgttctg
     2641 taagccctgg acatccatct gcatttcgtt cagataaaga ctgggccttc cgccccgcca
     2701 cgacaaagac gcggtacccc ccggaccctg cccagctacg atctgcagac cgggcacctt
     2761 cccctgcaaa taagtgaaga tatcggggta cgagctggcc agctgatcgt tgaccaggtc
     2821 gtactcgtac gcatccccac ccgaaaacat ccccgacgca taacgctcgt cgagcttctc
     2881 ggcatccgtt ttaacatgtc ccctcaccgt caccgattcc agggtcttca ctttctggtc
     2941 ctctatcggc ttggccctgg ccagttcctg cacgacaaat ttattgcgcc gcagatacgc
     3001 cgaatcctcc gccgtccacc catcgaacga gctcaccaac ggtttggctg acctcactgc
     3061 tctcagtaag ccggtgttga aggtcaccgc tgcttcattc gaaaggttgc ggttgatatt
     3121 gaattgataa taagccttgg ccgtatcata aaagaccaga ccggataccc cgaat
//
LOCUS       DASYSV010000569         6881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_626179, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000569 DASYSV010000000
VERSION     DASYSV010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626179"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VGQ51_17765"
     CDS             <1..201
                     /locus_tag="VGQ51_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038490.1"
                     /translation="EAAKDEASRGGIAGEAAKDEASRGGIAGEAALADALVARAGAGS
                     QLRYLLKKSIVRCQASSAAALS"
     gene            complement(132..1019)
                     /locus_tag="VGQ51_17770"
     CDS             complement(132..1019)
                     /locus_tag="VGQ51_17770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV9038491.1"
                     /translation="MSAIKKEDVRQEVFDLYDAYAHNRLSRRDFMESLSIYAVGGLTV
                     PALMSFLMPDYKNTLQIKTNDPRIVSDYIHYDSPQGGGSIKALLSKPSGATKKLGGVV
                     VVHENRGLNPYIEDVGRRLAVAGFISIAPDALTPLGGYPGNDDQGREMQAKRDKNEML
                     QNFIDAFEYLRKQPDCNGKVGVVGFCFGGGIVNLMAEKLPDLAAGVPFYGAQPPLDQV
                     SQIHAPLLIQYAGLDTRVNDGWPAYEAALKENKKEYQVYIYPNVNHGFHNDTTPRYDK
                     AAAELAWQRTIDFFNKYLS"
     gene            complement(1043..2122)
                     /locus_tag="VGQ51_17775"
     CDS             complement(1043..2122)
                     /locus_tag="VGQ51_17775"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV9038492.1"
                     /translation="MIKHIIIAGFLLPLTLHGQSPAKGQSSPIGHPPAKGQSSPIGHP
                     TPIGKIELYDKTAATLIDTNAIIEVLGTHYQWAEGPVWVPSRQMLLFSDVLANTAYVW
                     KAPDTPTIYLAPSGYSGTAVRNGENGSNGLTLDRNGRLLLCQSGDRRLVRMNAPLDSP
                     RPDFTILASNYKGKKFNSPNDVVVDSRNNIYFTDPIYGLPGKEKDPTRELTFEGVYRI
                     SNSGQLTLLIDSIARPNGIALSPDEKTLYVGSSDDPHTRWYAYHIDDKGKIMGGGILL
                     DGTALKARASVKQGADGFKVDDRGNIWSAGPDGINIISPRGRLLGLIRIYHRRVSNCA
                     FDRRKKLLYITATDLLLRVKLTNIP"
     gene            complement(2141..2713)
                     /locus_tag="VGQ51_17780"
     CDS             complement(2141..2713)
                     /locus_tag="VGQ51_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEV9038493.1"
                     /translation="MKKNRLEAFSDGVIAIIITIMVLELHVPHGAQIKDLAPVVPTFV
                     AYLLSFTLVGIYWNNHHHMIYAVDRINGMILWANLHLLFWLSLMPFATSWMGENYLSR
                     WPVALYGGVLAMCGFAYTLLCKLLIREAGPTSRLALALEKDWKGRLSQIIYLVAIALA
                     FVDPRIALGMYVIVALLWFVPDRRIEKTMD"
     gene            complement(2782..3462)
                     /locus_tag="VGQ51_17785"
     CDS             complement(2782..3462)
                     /locus_tag="VGQ51_17785"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEV9038494.1"
                     /translation="MPENSEYYPLVMQRYPARPFPAGEYEDFIPALLRVFEEKSFNAG
                     EFSPFPLHIIVDYIQHTHVYYLSKKLPEIEQSILLLSGQYELHHPILIALQNFFRHYV
                     QDLSEHIRAEETRLLPYIDLLQEASRTPAGFSRWLMAHDEYSINRFLSDHHDTEDELK
                     DIRQTIRLYEPPKTNESLYRILLSQLQTFEQDLSVHAHIEEEVLIPKARALEKELATE
                     ADTFAPDN"
     gene            complement(3493..6183)
                     /locus_tag="VGQ51_17790"
     CDS             complement(3493..6183)
                     /locus_tag="VGQ51_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEV9038495.1"
                     /translation="MRLKYWLAALCLLALNTLQSQPTIPSPKDVFGFNIGDNYMLANY
                     TQTIAYIKKIAAASDRAKFTVIGKTEEGRDQFMLIVSSPENLKHLDHYKEISQHLAHA
                     EDLTDSQAHTMAGEGKAVVWIDGGLHATEVVATQQLTEIAYQLLTRTDPETLNILDKD
                     IILITHVNPDGMELVSDWYMRNPKPESRSLDEVPRLYEKYAGHDNNRDFYMLNLKETQ
                     NICRQLFVEWLPQIMYNHHQAGPAGSIVAGPPFRDPFNYVFDPLVMTSLSAVGAAMNN
                     RLNEEGKPGYTDLAGSTYSTWYNGGLRTTTYFHNIIGLLTEIIGGPNPTEVPLVPNRL
                     IPNSATPNPITPQKWLFRQSIDYEVSLNYAVLDYAARYHDELLYNIYRMGRNSIDRGS
                     RDNWTLSPKRVAGIDSLAAHDAATSRRGAASADSMRTSRRAAPADIVARGPGVLPTRF
                     YDSVLKNPANRDARGYIIPSDQPDFPTAIRFINALIGDGIAVDKATAPFTVQGATYPA
                     GSFVVKTDQAFRPHVLDMFEPQDHPNDFAYPGGPPIAPYDAAGWTLAYTMGIKFDRIL
                     DGFDGPFNRIPYGELQTATSPTAKDGWLDARSNASFTEVNRLLKEGKTVYRSTTNGNF
                     YIGSHRPQNTAKISRARIAIWDNYGGSISSGWLRFIMEQYQFPADIIYAPTIDSGNLR
                     DKYDVIVFVTGAIQPPREEAPRFGFGGRMPSPGSVPEEWRSHIGRITADKSIPQLKKF
                     LEAGGHIVTIGGSTSLAYHLGLPVRDALVEMANGQERRLSNEKFYIPGSVLRISVDST
                     LQPGWGMPSEADVYFDQSPVFHISPEAAANGSVRPIAWFATDKPLRSGWAWGQAYLRD
                     GVAAFEAKIGQGMLYAFGPEITFRSQTHGTFKLLFNELYK"
     gene            6237..6797
                     /locus_tag="VGQ51_17795"
     CDS             6237..6797
                     /locus_tag="VGQ51_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038496.1"
                     /translation="MKLFIGWLIVFMIGASNGMGMSPGADSTGKDTTKYTSFKDLPLK
                     PQRKVAFTTTEGTWTSLDVSPDGKTIVFDMMGDLYTMPITGGKATQLTRGIAFDSHPR
                     WSPDGRKILFSSDRSGAENIWWIDPEKKDTFQVTKERDQNFPSAAYNGRLYEGSTCNE
                     VYPGKRALDRNEWNFERPANTTGIAD"
BASE COUNT         1435 a         1601 c         2231 g         1614 t
ORIGIN      
        1 gaggcggcaa aggatgaggc ttcgcgtggc gggatcgccg gggaggcggc aaaggatgag
       61 gcttcgcgtg gcgggatcgc cggggaggcg gcgttggcgg atgcattggt cgcgcgggcc
      121 ggcgcgggtt ctcagctgag atatttattg aaaaaatcga tcgtccgttg ccaggccagt
      181 tctgccgcag ccttgtcata gcgcggggtg gtatcgttgt gaaaaccgtg gttgacgttg
      241 ggatagatat agacctggta ttctttttta ttctctttga gggcggcttc ataagctggc
      301 caaccgtcat tgacgcgcgt gtcgagaccg gcatattgaa tgagcagcgg ggcatgtatc
      361 tgcgagacct ggtccagcgg tggttgggcg ccatagaatg ggactcctgc ggcgagatcg
      421 gggagttttt cggccatcag attgacgata ccgccgccga aacaaaatcc aactactcct
      481 actttgccat tacaatcggg ttgttttctc aggtattcga aggcgtcgat gaagttttgg
      541 agcatttcat tcttgtctct tttagcctgc atctcgcgac cctgatcgtc attcccgggg
      601 tagccgccta gcggcgtcag cgcatcggga gcgatggaga taaaacccgc cacggccagg
      661 cgtctgccga catcttcgat gtagggattg aggccgcggt tctcatggac gacgacgaca
      721 ccgccgagct ttttggtggc gccggagggt ttggacagta atgctttgat agatccgccg
      781 ccctgcggag agtcataatg gatataatcg gagacgatgc gcggatcgtt ggtctttatt
      841 tgaagggtgt tcttgtagtc gggcatgaga aagctcatga gtgcgggcac agtgaggccg
      901 ccgacggcat agatggagag agactccatg aaatcgcggc gcgagagacg gttgtgcgcg
      961 taggcgtcgt agagatcgaa gacttcctgg cggacgtctt cttttttgat ggcagacata
     1021 tggatgtggg tttgacttaa agttaaggaa tattggtgag tttgaccctg agcagaagat
     1081 cggtggcggt gatatagagc agctttttcc gtcggtcgaa tgcacagttg gagacccggc
     1141 ggtgatagat cctgatcagc cccagcagcc tgccccgggg agagatgata tttatcccgt
     1201 ccggtccggc gctccagatg ttaccccgat catcgacctt aaagccatcg gctccctgtt
     1261 tgacggatgc acgggccttc aatgcggtgc cgtcgagcaa tatgccgccg cccatgatct
     1321 tgcctttgtc gtcgatatgg taggcatacc agcgcgtatg cggatcgtcg ctgctgccga
     1381 catacagggt tttttcgtca ggcgaaagtg caatcccgtt tggccgggcg atgctatcga
     1441 tgagtaatgt gagctggcct gagttgctga tcctgtatac tccttcaaag gtcaattctc
     1501 ttgtggggtc tttttccttg ccgggcaggc cgtagatagg atcggtaaaa tagatgttat
     1561 tcctgctgtc gacgacgacg tcgttaggac tattgaactt cttcccttta tagttggagg
     1621 cgagtatggt gaaatccggc cggggcgaat cgagtggggc attcatccgg accaatcgcc
     1681 tgtcgccgga ctgacaaaga aggagccggc cattgcgatc gagtgtcagg ccattggatc
     1741 cgttttcgcc gttacgaacg gctgtacccg aataaccgga aggggcaagg taaattgtgg
     1801 gggtgtctgg tgctttccag acataagcgg tgttggccag tacgtcagag aatagcagca
     1861 tttggcgcga gggcacccaa acggggcctt cggcccactg ataatgagtc ccgaggactt
     1921 cgataatggc attggtgtcg ataagggtgg ctgcggtctt gtcgtagagc tcgatcttgc
     1981 cgatgggggt ggggtgtccg atgggggagg actgtccttt ggcgggggga tgtccgatgg
     2041 gggaggactg tcctttggcg ggggactggc cgtgtagtgt caggggcagt aggaaacctg
     2101 ctattattat gtgcttgatc atatgcctat ggaaattgtt tcagtccatg gtcttttcta
     2161 tccggcggtc cggaacgaac catagcagcg ccacaatcac atacattccc agcgcgatcc
     2221 gggggtccac aaaggccaac gcaatggcta ccaggtatat aatttgggag agccgtccct
     2281 tccaatcctt ttcaagagcg agtgccagtc ttgatgtcgg tcccgcctcc cggatcagca
     2341 gtttgcacag gagggtatag gcgaatccgc acatcgccaa tactccgcca tacagggcta
     2401 ccggccacct ggaaagatag ttctcgccca tccaggatgt cgcgaatggc atcagcgata
     2461 gccaaaacag caaatgcagg tttgcccata ggatcattcc gttgatgcgg tcgacggcat
     2521 agatcatatg atggtggtta ttccagtaga tacccacgag ggtaaagctc aggaggtagg
     2581 cgacgaaagt gggcactacg ggagccaggt ctttaatttg ggctccatga gggacatgca
     2641 actccagcac cataatagta ataatgatcg ctatcacgcc gtcgctgaat gcttccaaac
     2701 ggttcttttt catatccggc ggtggagtta gttgttgggg gccatcgggt ggaattagtt
     2761 gtttggggcc atcgggtgga attagttgtc gggggcgaag gtatcggcct ctgtagcgag
     2821 ttccttctcc agcgcgcgcg ccttgggaat gagcacttcc tcttcgatat gggcgtggac
     2881 ggagaggtcc tgctcgaagg tctgcagctg cgagagcagg atgcggtaga gcgattcatt
     2941 tgttttggga ggctcgtaga gacggatggt ctggcggata tccttgagct cgtcttcggt
     3001 gtcgtggtgg tcgctcagga atcggttgat agaatactcg tcatgagcca tcagccagcg
     3061 tgagaagcct gccggggtcc gggaagcctc ctggaggaga tcgatatagg gcagaaggcg
     3121 ggtttcttcg gcgcggatgt gttcggagag gtcctgcaca tagtggcgga agaagttttg
     3181 gagggctatg aggatcgggt ggtgcaattc atattggccg gacaggagga ggatgctttg
     3241 ctcgatctcg ggaagcttct tcgagagata gtagacgtgg gtatgctgga tgtagtcgac
     3301 gatgatgtgg agcgggaagg gagagaactc cccggcattg aaggatttct cttcgaagac
     3361 ccgaagcagg gccgggatga aatcctcgta ttcgccggcc ggaaatggcc gggccggata
     3421 gcgctgcatc accagtgggt agtattcgct gttttcaggc atgttatccc ctgttgttgg
     3481 acaaatgtag tattatttgt acaattcgtt gaatagcaat ttaaacgttc catgcgtttg
     3541 ggaacggaag gtgatctcgg ggccgaatgc atacagcata ccctggccga tctttgcctc
     3601 aaaggccgct acaccgtcgc ggagataagc ctgcccccag gcccagccgc tgcgcaaagg
     3661 tttgtccgta gcgaaccagg cgatgggccg gacgctgcca tttgcggccg cttcggggct
     3721 gatgtgaaag acggggctct ggtcaaaata gacatcggcc tctgagggca tcccccagcc
     3781 cggctgcagg gtagagtcga cgcttatacg aagcacgctg cccgggatat agaatttctc
     3841 attggagaga cgtctttcct gtccgttagc catttccacc agcgcgtcgc gtacgggcag
     3901 acccaggtga taggcgaggg aggtgctgcc gccgatggtt acgatatgac cgcccgcttc
     3961 aaggaatttc ttcagctggg ggatggattt gtctgctgta atgcgaccga tatgcgaacg
     4021 ccattcttcg ggtacgctcc cgggactcgg catccttcct ccgaaaccga agcgtggcgc
     4081 ctcttcacgg ggcggctgga tcgcaccggt gacgaagacg atgacatcgt atttatcgcg
     4141 cagattgccg gagtcgatag tcggagcgta gatgatatcc gcgggaaact ggtattgctc
     4201 cataatgaac cgcagccatc cggaagaaat ggaacctcca tagttgtccc agatcgcgat
     4261 gcgggcccgg cttattttgg ccgtattttg gggacggtgg ctgcctatgt agaaattgcc
     4321 gttggtagtg ctgcgataaa cggtcttgcc ttccttcagc aggcggttga cctcggtgaa
     4381 ggaggcattg ctgcgtgcat ccagccagcc atcctttgcc gtcggtgagg ttgcggtttg
     4441 cagttcacca tagggaatcc ggttgaaggg gccgtcgaaa ccgtcgagga tgcgatcgaa
     4501 ttttattccc atcgtatagg caagcgtcca tccggccgcg tcatagggtg cgataggagg
     4561 accgcccgga taggcgaaat cgttaggatg gtcctgcggc tcgaacatgt cgaggacatg
     4621 tggacggaag gcctggtcgg tcttgacgac aaaggaaccg gcaggatagg tggcaccctg
     4681 gactgtgaat ggggcggtag ccttgtcgac tgcgatgccg tcaccgatca gcgcgttgat
     4741 gaagcggata gccgtaggga aatcgggttg gtcggatggg atgatgtagc cgcgggcatc
     4801 gcggttggcg gggtttttca ggacgctgtc gtagaaccgg gtgggcagga cgccgggacc
     4861 gcgggcgaca atgtcggccg gggcagccct tcggctggtt cgcatggaat cggcgctggc
     4921 ggcgccgcga cgggaagtgg cggcatcatg ggcggcgagg ctgtcgatcc cggcgacgcg
     4981 tttgggggaa agggtccagt tgtcacgact gccccgatct atagaattcc gccccatccg
     5041 gtaaatattg tacagcaact cgtcgtgata gcgtgcggca tagtcgagga cggcataatt
     5101 gagcgatacc tcgtaatcga tcgattgcct gaacagccat ttctgcggag tgatgggatt
     5161 gggtgtagcg ctattgggga tgagacggtt gggtaccaat gggacctccg tcgggttggg
     5221 gccgccgatg atctcggtca ggagaccgat gatattgtga aaatatgtgg tggtgcgcag
     5281 tcccccgtta taccacgtgg aataggtgga accggcgaga tcggtatagc cgggcttacc
     5341 ttcttcattg aggcgattgt tcatcgcggc gccgactgcg ctcaggctgg tcatgaccag
     5401 cggatcgaag acatagttga agggatcgcg gaaaggaggg ccggctacga tggaaccggc
     5461 gggtcctgcc tgatggtggt tgtacatgat ctgggggagc cattcgacaa acagctggcg
     5521 gcagatattc tgtgtctctt tgagattgag catatagaaa tccctgttgt tatcatgacc
     5581 ggcatatttc tcatagagac gcggcacttc atcgagcgag cggctttccg gtttgggatt
     5641 gcgcatatac cagtccgaga ccagctccat tccgtcgggg ttgacatggg tgatcaggat
     5701 gatgtccttg tcgagtatat tcaaggtctc gggatcggtg cgggtcagca actggtaagc
     5761 gatctccgtg agctgttggg tagcgacgac ttcggtggca tggagaccac catcgatcca
     5821 gacgacggcc tttccttcgc ccgccatggt gtgtgcctgt gaatcggtga ggtcttcggc
     5881 gtgggcgagg tgttgggaaa tttctttata atgatcgagg tgtttgaggt tttcgggaga
     5941 ggagacgatg agcatgaact ggtcacggcc ttcttccgtc ttgccgataa cggtgaattt
     6001 ggcgcggtcg gaggcagcgg cgatcttttt gatataggcg atggtttggg tatagttggc
     6061 gagcatatag ttgtcgccga tgttaaatcc gaagacgtct ttgggtgacg ggatggtggg
     6121 ttggctttgt aacgtattga gcgcaagcag gcagagtgcg gcaagccaat atttcagtct
     6181 catgtgcagg aggtttggct gaatatacgt aatttacctg tccaaacatt aactgcatga
     6241 aactctttat cgggtggcta attgtcttta tgatcggggc gtcgaatggg atgggaatga
     6301 gccctggcgc cgactccacc ggcaaggata ccacgaaata tacatctttt aaagacctgc
     6361 cgctgaaacc tcagcgcaag gtcgctttta ccacgaccga agggacctgg acgtcgttgg
     6421 atgtcagtcc tgacggcaag acgatcgtgt tcgacatgat gggtgatctg tatacgatgc
     6481 ccatcaccgg tggaaaggcg acgcagctga cccgtggcat cgccttcgac tcgcatccgc
     6541 gatggagtcc ggacggcagg aagatacttt tttcttccga ccgcagcgga gcggagaata
     6601 tctggtggat cgatccggag aaaaaggaca ccttccaggt gacgaaggag cgggatcaga
     6661 atttcccaag cgcggcctac aatgggcggt tgtatgaggg cagcacgtgt aatgaagtat
     6721 acccggggaa gagagcattg gacaggaatg agtggaattt cgagcgtccg gcgaatacaa
     6781 cggggatagc ggactgaggt tatgaaaaga tccattctta ttatttgctt attggctggc
     6841 gtgggagcgg cggcgcagac ctgtacgggg ggattgggtg a
//
LOCUS       DASYSV010000570         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2422365, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000570 DASYSV010000000
VERSION     DASYSV010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2422365"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="VGQ51_17800"
     CDS             <1..1371
                     /locus_tag="VGQ51_17800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9038497.1"
                     /translation="LREGDVVRFVKDISLSFVDLAERKNIRFSLKEEGGPLITLFDHD
                     KLERILFNLLSNAFKFTPENGSVTVDVRAVRTMPPSPDAGEAHESLLTIAIRDTGIGI
                     EPDKQEKIFERFFQNEVPDTILNQGSGIGLSITQEFVKMHGGRLSVTSEVNKGSCFTV
                     ELPFREIELSGTTIPQFDLPDPPPATRPGRAISAPKNAPTILIVEDNEDFRFYLKDNL
                     RGLYKIIEASDGKEGWKKVLGEQPQLVVSDISMPHMDGIQLCRKIRTDERTRQIPVIL
                     LTAMSGETIELEGLQTGATDYISKPFNFEVLLSKIRNVVEYNETVRKTWQRRVETRPA
                     PVEMTSPDDLFLREVLEYIEKNIGDPGLSVEALCQRFHSSRSTFYKRLLLLTGKTPLE
                     FIRHIRLQRAADLLEKSQLTVAEIAYTVGFNNPKYFTHYFKSAFDCIPSAYRAGRKEA
                     GQRTTE"
     gene            1403..2125
                     /locus_tag="VGQ51_17805"
     CDS             1403..2125
                     /locus_tag="VGQ51_17805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV9038498.1"
                     /translation="MPNPQFIQHVIHDIQGDFLGVAGLCQSMKKGLEEKKDIKGLLDI
                     LIENCRTYKYKLGNFLEFTRMQAGLSKTVRGSVDIRRLLNQVIDEYDTLWIEKDARIR
                     LEISPRLPQEFFCDEGKIQLIVANLFVNAVHFSPPGSTITLVSAPAAPDAITIASTPA
                     APDRWGITITDTGQGMTDQQLGAAFTTEPAQRAVLHNSAGLGLIISRWLAEEVLGGKM
                     VLKSRPGEGTCVHLDLPFYPYE"
     gene            2240..>2937
                     /locus_tag="VGQ51_17810"
     CDS             2240..>2937
                     /locus_tag="VGQ51_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038499.1"
                     /translation="MRISNLFTIGTSGINDIETRNRIVLVNKISLAFGLALLVITPTF
                     CSFANWKLSVVLPLTIEFIANSAVLLLNHKKHHTAAAILLYLLQCVAIVYFSILLGRL
                     IRLELAIILLISILYLIFREKHLRKLALGAAIIELAILEVVHFVSSTSPVILLTYRQS
                     CILHLLIDISAISVIIIVSKPYVQSNDSNAALKRANHFIRLFVAEISHELRTPLDNIH
                     QVVQLLKKEVQDDDR"
BASE COUNT          714 a          872 c          714 g          637 t
ORIGIN      
        1 ctccgcgaag gagacgtggt gcggttcgta aaggatatat cgctttcctt cgtcgacctg
       61 gccgaaagga aaaatatccg tttctccctg aaagaggaag gcggcccgct gatcaccctt
      121 ttcgatcatg acaagctcga gcgcatcctt ttcaatctcc tgagcaacgc atttaaattc
      181 acgcccgaga acgggtctgt cacagtcgat gtccgcgccg tacggacaat gcccccctcc
      241 cccgacgccg gcgaagcgca cgagtcactc ctgactatcg cgatacggga caccggtatc
      301 ggtatcgagc ccgacaaaca ggaaaagata ttcgagcgct tcttccagaa tgaagtaccc
      361 gacaccatcc tcaaccaggg cagcggtatc ggactttcga tcacccagga attcgtgaag
      421 atgcatggcg gccggctgtc ggtgacaagc gaggtcaata aaggcagctg cttcacggtc
      481 gaactgcctt tccgggagat agagctatcg gggactacca tcccccaatt cgatttgcct
      541 gatccgccgc ctgccacccg cccggggcgc gccatcagcg cacccaaaaa cgcccccacc
      601 atcctcatcg tggaagacaa cgaggatttc cgtttctatt tgaaagacaa tctccgcggt
      661 ctatataaaa taatcgaagc ctccgacggc aaggaaggat ggaaaaaggt cctcggcgaa
      721 caaccccagc tggtcgtgag cgatatttcg atgccgcata tggacggcat ccaattgtgc
      781 cgaaagatcc gtaccgatga acgaacccgt cagatccccg tgatccttct caccgcgatg
      841 agcggtgaga cgatagagct ggaaggcctg cagaccggcg ccaccgatta tatcagcaaa
      901 cccttcaatt tcgaagtgct gctgtctaag atcaggaatg tcgtcgaata caatgagacc
      961 gtccgcaaga cgtggcagcg ccgcgtggag acgagacccg cgcccgtgga aatgacctcg
     1021 cccgacgacc tgttcctgcg cgaggtgctc gaatacatcg agaagaatat cggcgatccc
     1081 gggctttcgg tcgaggcgct ttgccagcgg ttccattcca gccgaagcac tttttacaaa
     1141 cggctccttc tgctgacggg caagacgcct ttagaattca tccggcatat ccgcctgcaa
     1201 cgtgcggccg atctgctgga gaaaagccag ctcaccgtgg cggagatcgc ctatacggtc
     1261 gggttcaata atcccaaata ctttacgcat tacttcaaat cggcttttga ctgtataccg
     1321 tcggcctata gggcgggcag gaaggaggct ggtcagagga ccaccgaata aaaccgtaaa
     1381 tttagcccgt agcgtctgct ccatgccgaa tcctcaattc atccagcacg tgatccacga
     1441 catccaggga gacttcctgg gagtggcggg attatgtcaa agcatgaaaa aagggctgga
     1501 agaaaaaaag gatataaaag gtctgctcga tatcctcatc gagaactgca ggacctataa
     1561 atacaaacta ggcaatttcc tggagtttac ccgcatgcag gcggggttga gcaagacggt
     1621 gagggggtcg gtcgatatcc ggcggctgct caaccaggtg atagacgaat atgataccct
     1681 ttggatcgag aaggacgcac gcatccgtct cgagatctct ccccggctgc cgcaggaatt
     1741 cttctgtgac gaagggaaga tccagcttat agtcgccaat ctctttgtca atgccgttca
     1801 tttttcgccc ccgggcagca ccatcaccct cgtctccgcc cctgcagccc ccgacgctat
     1861 caccatcgcc tccacccctg cagcccccga ccgctggggc ataaccatta ccgataccgg
     1921 ccagggaatg acggatcaac agctcggggc cgccttcacc accgaacccg cccaacgggc
     1981 cgtgctccac aatagcgccg gcctgggcct gatcatcagc cgatggctgg cggaggaggt
     2041 gctcggcggc aaaatggtgc taaaaagccg ccccggcgag ggtacttgcg tccacctcga
     2101 cctacccttt tatccatacg aatagcggat tcatccatag aaaaatcgat ttagctttag
     2161 attattgcat ttcttacggt aatcatttaa cttccttctc atctaaactt tacctattaa
     2221 tgaattattt gacaccgcta tgaggatctc aaatcttttt accattggga cgtcagggat
     2281 caatgatata gagaccagga acaggatcgt cttagtcaat aagatcagtc tggctttcgg
     2341 actggcgctg ctggtcatta cccctacctt ctgctcattc gcgaattgga aactgtcggt
     2401 cgtccttcct ctcaccatag aattcatcgc caacagcgcc gtactgctgc tgaatcataa
     2461 aaaacatcat acggccgccg ctatcctgct gtatcttctt caatgcgtgg ccatcgtcta
     2521 tttcagcatc ctgctcggaa gattgatccg gctcgagctg gccattatac ttcttatctc
     2581 catcctctac ctgatattca gggaaaagca cctccgtaaa ctggctcttg gcgcagccat
     2641 catcgagctg gcgatcctcg aggtcgtaca tttcgtctct tccacctccc cagtcatcct
     2701 gctcacctac agacaaagct gtatcctcca cctgctgatc gatatttccg ccattagcgt
     2761 catcatcatc gtcagcaagc cctacgtgca aagcaacgac tccaacgccg cgctcaaacg
     2821 ggccaatcat ttcatccgcc tgttcgtcgc agagatctcg cacgagctca ggacgccgct
     2881 cgacaatatc caccaggtgg tccagctgtt gaaaaaagaa gtgcaggacg acgatcg
//
LOCUS       DASYSV010000571         3479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1013105, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000571 DASYSV010000000
VERSION     DASYSV010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013105"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VGQ51_17815"
     CDS             <1..639
                     /locus_tag="VGQ51_17815"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV9038500.1"
                     /translation="LGFLTALFFAILILRCTWFYLQPHQDSQTFFYFNTVFRMDSLVL
                     GATLTQLHFEGKQIPRRTFNAIFLLSGFSLMIAFIAIRPPYPWHPFFETAGYSLLALF
                     AACLIHRSVAYPNSILTRILSNPILRFIGKISFGLYVFHFLIYQIAGPHIRSWLAAGT
                     EEHSRLSAIGSAITCLLITAATACLSYYYFERPFLRLKKNANVQNARNPAVE"
     gene            complement(602..1735)
                     /locus_tag="VGQ51_17820"
     CDS             complement(602..1735)
                     /locus_tag="VGQ51_17820"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV9038501.1"
                     /translation="MKEAGRKKSLIRRVAYVLYLFVAVFLLLEVALRIYNPFHFRLKG
                     DKILLPVNVRETITNRINPKLDATIVNTRNSLGFRGPEPPADWGRHLTLITIGGSTTE
                     CHFLNDDKTWPYLLGRDLSDSFRDVWLNNAGLDGHSTFGHRVLLNDHIKQLRPSVVVF
                     LTGINDVETDVPSFHDKLNTRNAYPDFRHWLFNNSEVLALGLNFVRGWRAQRFNNTTN
                     DMLVLDSGRRLPLQETVMGKRLAEQAGFLKGYRQRLEDLADTCLAWHIVPVFMTQPNQ
                     FGFGRDPLTGADLALYPVDPHDSGLNGALMWRILERYNDVVREIGAEKQVPVIDLARM
                     MPKNSLYFYDMSHFTNRGAEKVASLISGPLTVILRRDFARFVR"
     gene            complement(1737..>3479)
                     /locus_tag="VGQ51_17825"
     CDS             complement(1737..>3479)
                     /locus_tag="VGQ51_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038502.1"
                     /translation="RNYNFEWTFNSQASRESMAHFLDSIPNGYWVTFKNVPSYVVSAN
                     QYASDWAADTLVYGKGNTLYDKLKALGLTIDSFSRPRVFLAIFKKGDPTFALQQKWSV
                     KDTDAITMESFCPGPIYQGSLASPKWGPAKAWSTVHWRGADPVNSTTDTVGVQVVGID
                     TLGNSTPLFRMSRSQQDLNISTINAKQYPYLQLKLSTYDSVHGAPYQLQNWRVNYTPV
                     PEGALAPNILLKGPDTVTLGQPMDFAIAFKNVSQYAFDSLKIQLYVLDKSNVMHPINM
                     PRRKPLISGDTVTLEYLIDTKTLSGQNSIYVEFNPGGDQPEQFTFNNFMYKSFFVKGD
                     SRAPTMDVTFDNVHILNNDIVSAKPHIQIRLQSLSQYLLLTDTSTIQVQVRFPDGSMH
                     NYGFSSDTLRFTPASGSNNNVAIVDFYPAFTNQINPQGDTYQLIVTGKDESGTMAGAT
                     PYKVDFQIITKAMISNMLNYPNPFTTSTRFVFTITGSEVPQNIKIQIMTITGKVVREI
                     TREELGPLHVGRNITEFAWNGTDMYGQRLANGVYLYHVVTNLNGKSLQKYTGAGDNTD
                     KFFNNGYGKMYLMK"
BASE COUNT          810 a         1046 c          789 g          834 t
ORIGIN      
        1 ctcggatttc tgactgccct gttcttcgcg atccttatcc taagatgcac ctggttctat
       61 cttcagccac accaggacag ccaaacattt ttttatttca ataccgtgtt ccgcatggat
      121 tcgctggtat tgggcgccac cctcacccaa ttgcacttcg aaggaaaaca gatcccccgc
      181 cgcacattca acgccatctt ccttctcagc gggttctcac tgatgattgc cttcatcgcg
      241 atccggcccc catatccatg gcatcccttt ttcgagaccg cgggctacag cctgctcgcc
      301 ctcttcgcgg catgcctgat ccatcgctcg gtcgcatacc cgaattccat cctgacaagg
      361 atcctaagca accccattct tcgtttcatc ggaaaaatat cctttggact gtacgtcttt
      421 cattttctca tttatcaaat cgcaggtccc catatcagaa gctggcttgc cgcgggaacc
      481 gaagagcata gccgcctctc cgctatcggc tcggcaataa catgcctgct gatcacggcc
      541 gccacggcct gcctcagcta ttattatttc gaacgaccct tccttaggtt gaaaaagaat
      601 gctaacgtac aaaacgcgcg aaatcccgcc gtagaatgac ggtcagcgga cctgagatca
      661 gcgaagccac cttctccgct ccgcgattcg taaaatgtga catatcataa aaatacaggc
      721 tgttcttcgg catcatccgc gccagatcga tcaccggcac ctgcttctcc gcgccaatct
      781 cacggaccac gtcattataa cgttccaata tccgccacat caatgcccca ttgagaccgc
      841 tgtcatgcgg gtccacagga tacagcgcca ggtcggcacc tgtcagcggg tccctcccga
      901 aaccaaactg attaggctgt gtcatgaaca ccggaacaat atgccacgcc agacaggtat
      961 ccgccagatc ctccaaccgc tgcctgtatc ccttcaaaaa tcccgcttgc tccgccaggc
     1021 gcttacccat caccgtctcc tgcaacggca accgccgccc actatccaat accagcatgt
     1081 cattcgtagt attgttgaac cgttgcgccc gccaacccct cacgaaattc aatcccagcg
     1141 ccaacacctc gctattattg aaaagccaat gccgaaagtc gggataagca ttccgcgtat
     1201 tcaatttgtc atggaaggac ggcacatccg tctccacgtc attgataccc gtcaggaaca
     1261 caaccaccga cggccgcagc tgcttgatat ggtcattcag caaaacccga tgtccgaagg
     1321 tggaatgtcc gtccagcccg gcattgttca accagacgtc gcgaaaggaa tcggacagat
     1381 cacggcccag caaataaggc caggtcttgt catcattgag aaaatgacat tcggtcgtac
     1441 tgcctccgat ggtgatcaaa gtaagatgcc gcccccaatc cgccggcggc tcgggacccc
     1501 gaaaacccag actattgcgg gtattgacga tcgtcgcgtc cagcttggga ttgatccggt
     1561 tagtgatcgt ctcccgcacg ttcacgggta acaatatctt gtcacccttt agccggaaat
     1621 ggaaaggatt ataaatgcgc aacgccacct ccagcaacaa aaacacggcg acaaacaggt
     1681 ataagacata cgctacccgc ctgatcaggc tcttttttct tccggcttct ttcattttac
     1741 ttcatcagat acatcttccc atacccgtta ttgaaaaatt tatcggtatt atccccggca
     1801 cccgtatact tctgcagcga tttcccgttc aggttcgtca cgacatgata caaatatacg
     1861 ccattggcca ggcgctgtcc gtacatatcc gtaccattcc acgcgaattc ggtaatgttt
     1921 cgcccgacat gcaacggacc cagctcttcc ctggttatct cacgaaccac cttcccggtg
     1981 atcgtcatga tctggatctt gatgttctgc ggcacctcgc tgccggtgat cgtgaacacg
     2041 aatctcgtcg aagtcgtgaa aggattggga tagttgagca tattcgagat catcgccttc
     2101 gtgatgatct ggaaatccac cttatagggc gtggccccag ccatagtccc cgattcatcc
     2161 ttaccggtga cgatcagctg ataggtatcc ccctgcgggt tgatctggtt ggtaaaggcc
     2221 ggatagaaat ccacgatggc gacattgtta ttgcttccgc tagccggagt gaatcgcaaa
     2281 gtatcggaac tgaatccata gttatgcatc gacccatccg ggaaacgcac ctgcacctgg
     2341 atcgtcgatg tatccgtcag cagcaggtat tgcgacaggc tctgcagcct gatctggata
     2401 tgcggtttgg ccgagacgat gtcattgttc aatatatgca cgttgtcgaa agtgacgtcc
     2461 atcgtcggcg cacggctgtc ccctttgacg aaaaagctct tgtacatgaa attgttgaac
     2521 gtgaactgtt ccggctgatc cccgccgggg ttgaattcca catagatgga attctggccc
     2581 gacagcgtct tcgtatcgat caggtattcc agcgtcacgg tatctccgct gatcagcggc
     2641 ttccttcgcg gcatgttgat aggatgcatg acattgctct tatccaatac gtacagctgg
     2701 atcttcaaac tgtcgaacgc atactggctg acattcttga aggcaattgc gaaatccatc
     2761 ggctgtccca atgtcaccgt gtccggtccc ttcaacagga tattcggcgc cagcgcgcct
     2821 tccggcacgg gtgtatagtt gacccgccaa ttctgcagct gatagggcgc cccatggacc
     2881 gaatcatagg tcgacaactt caactgcaaa tagggatact gcttcgcatt gatggttgaa
     2941 atattcagat cctgctgact tcggctcatc ctgaacaatg gcgtggaatt tcccaacgtg
     3001 tcgatcccta ccacctgcac acccaccgta tccgttgtcg aattgaccgg atccgctccc
     3061 ctccaatgca ccgtgctcca ggcctttgcg ggaccccatt tcggtgaggc cagcgagccc
     3121 tggtatatcg gacccggaca gaatgactcc atcgtgatgg catcggtgtc tttcaccgac
     3181 cacttctgct gcagggcaaa ggtgggatcg ccctttttga agatcgccag gaacacccgc
     3241 ggccggctga aagaatcgat cgtcagaccc aaagccttca atttgtcata aagagtgttc
     3301 ccttttccgt acaccagcgt atcggccgcc cagtcggaag catactgatt cgccgatacc
     3361 acatacgagg gtacgttctt gaacgtcacc caatatccat tgggtatact gtccaggaaa
     3421 tgcgccatgg actcgcgcga ggcctggctg ttgaaggtcc attcgaaatt atagttccg
//
LOCUS       DASYSV010000572         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2038236, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000572 DASYSV010000000
VERSION     DASYSV010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038236"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VGQ51_17830"
     CDS             complement(<1..597)
                     /locus_tag="VGQ51_17830"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV9038503.1"
                     /translation="MRTAILGANGYIGKHLAHFLAAHGWDVHSYGHRNEPGLPGLRYT
                     PLDVREASAFKKLDTDVDLVFYLPGITGTIKGYDDYDNYIDVNEKGLLHLLDRLRRNR
                     QHHTRVVYPSSRLVYKGQKNLPLTEDAEKEFKTIYALNKWFGECALRQYAEYFDIPFT
                     IFRICVPYANLFEGAYGYGTVGFFLNKASSGNPISLYGT"
     gene            complement(572..1570)
                     /locus_tag="VGQ51_17835"
     CDS             complement(572..1570)
                     /locus_tag="VGQ51_17835"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV9038504.1"
                     /translation="MKKLNIGIMGCAAIARRSVIPAIQSLKDHFALTAIASRTSGKAK
                     EFAAEFGTKAVVGYDRLLEDPSIDVLYMPLPTGLHEEWLLRSLAAGKHVLVEKSLAID
                     HDSAGRMVDTARSKGMVLMEDFMFKYHGQHDYTWQLLRGERLGEIRLFRSQFGFPPLD
                     KDNFRYDPVVGGGALLDAGAYTVKATQWFLGQGLDVLNSVLYVDPATGADVLGNATLL
                     SKKGIVAQLSFGFDNFYQCNYEFWGSKGRLLAERAFTPKPTEQPAILFERQGEKEIHT
                     AKPDNHFINILLEFHQAVMQKNNQPWLDELLDQSRVLTEIKKRATKIVYENGHIRR"
     gene            complement(1573..>2284)
                     /locus_tag="VGQ51_17840"
     CDS             complement(1573..>2284)
                     /locus_tag="VGQ51_17840"
                     /inference="COORDINATES: protein motif:HMM:NF015521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NDP-hexose 2,3-dehydratase family protein"
                     /protein_id="HEV9038505.1"
                     /translation="LFRQFVSARPASEGQRLLLSALDDRHSLSTIEEIISWITRLKSK
                     YDLEVSRIPLNKVCNWTRTDDEIHHVDHKYFSVIGANVNISNREVINWSQPLVRSAQE
                     GLIAFIIKNINGVAHFLVQAKIEAGNFDILELAPTVQCLTGNYRKGQNEYEVPFIREV
                     LDCPIEKILYKTMQSEEGGRFYKEQNLNMIIEAGDDFPEEVPENFHWMTLNQLYGFIR
                     YNNYLNIQARSLLAAVAF"
BASE COUNT          479 a          601 c          644 g          560 t
ORIGIN      
        1 ggtgccataa agactgatgg gattgccgga agaagccttg ttcaggaaga aaccgaccgt
       61 cccatatcca taggcccctt cgaaaagatt ggcgtagggg acgcagatac ggaatatggt
      121 gaacggaata tcgaaatatt cagcgtattg ccgaagcgcg cactcgccga accatttgtt
      181 cagcgcgtag atggtcttga actccttttc tgcatcctcg gtcagcggaa gattcttttg
      241 gcccttgtat acgaggcggc tggaaggata aacgacccgc gtgtgatgct gtcggttgcg
      301 ccgcagcctg tccagcaaat gcagaagccc cttttcgttg acatcgatgt aattgtcata
      361 atcgtcatac cctttgatcg tgccggtgat cccgggaagg tagaatacga ggtcgacatc
      421 agtgtcgagt tttttgaatg ccgacgcctc gcggacatcg agcggcgtat accgcaggcc
      481 cggaagaccg ggttcgttcc ggtggccgta gctatggaca tcccatccat gcgcggcaag
      541 gaaatgagcc aggtgtttgc cgatgtatcc gttagcgcct aatatggccg ttctcataga
      601 cgatcttcgt tgcgcgtttt tttatttccg tcaggacccg gctttgatcg agcagctcgt
      661 ccagccaggg ctggttattt ttttgcatga ccgcctgatg gaactcaagc aggatattga
      721 tgaagtggtt gtccggtttc gcggtatgga tctccttttc accttgtctt tcgaaaagaa
      781 tagccggctg ttctgtcggt ttgggtgtga aggcccgttc ggcgaggagt cttcccttgc
      841 tgccccagaa ctcgtaatta cattgataga aattgtcgaa gccgaaggag agttgcgcca
      901 cgataccttt tttgctgaga agggtggcat tcccgagcac gtcggcgccg gtggccgggt
      961 cgacataaag aacagagttc aggacatcga ggccctgacc caggaaccat tgggtggcct
     1021 tcaccgtata ggcgcccgca tccagcaacg cgccgccgcc caccacaggg tcataccgga
     1081 aattgtcttt gtccagcggc ggaaatccga actggctccg gaacaacctg atctcaccca
     1141 gtctctcccc gcgaagcaac tgccaggtat agtcgtgctg accatggtat ttgaacatga
     1201 aatcttccat gagcaccatc cccttcgacc gcgccgtatc taccatccgt cctgcggaat
     1261 cgtgatcgat ggccagtgat ttttcgacga gcacgtgttt gcctgccgcc agcgaccgca
     1321 ggagccattc ctcgtggaga ccggtgggca gcggcatata gagcacgtcg atagaagggt
     1381 cttcgagaag acggtcgtat ccgactacgg cctttgtgcc gaattctgcg gcgaattctt
     1441 tcgccttgcc ggaggtgcgg ctggcaatgg cggtcagcgc aaaatggtcc ttcagcgatt
     1501 ggatggccgg gatcaccgat cgccgcgcaa tggcggcgca ccccatgatg ccgatgttca
     1561 attttttcat gatcagaatg cgacggcggc cagcaggctc ctggcctgga tgttcaggta
     1621 attgttatat cggatgaagc catagagctg gttgagcgtc atccagtgaa aattctcggg
     1681 gacctcctcc gggaaatcgt cgcccgcctc gatgatcatg ttcaggtttt gttccttata
     1741 gaaacgtcca ccttcttcgg actgcatcgt tttgtagagg atcttttcga tcgggcagtc
     1801 caatacctcg cggatgaacg gcacctcgta ttcgttctgc cctttgcgat agttgccggt
     1861 gagacattgt acggtggggg ccagctcgag gatatcgaag ttcccggctt cgatcttggc
     1921 ctggaccagg aaatgggcga ccccgttgat gttcttgatg atgaaggcga tcagcccttc
     1981 ctgcgccgac ctgacgaggg gttggctcca gttgatcacc tcgcggttgc tgatattgac
     2041 gttggcgccg atcaccgaaa aatatttgtg atcgacatgg tggatctcat catcggtgcg
     2101 ggtccagttg caaactttgt tcaacggtat cctcgaaact tcaaggtcat atttgctttt
     2161 gagccgcgtt atccaggata tgatctcctc gatagtggac agtgaatgcc ggtcatccag
     2221 cgccgagagc aatagtcgtt gtccctcact cgccgggcgt gccgatacaa actgccggaa
     2281 gaga
//
LOCUS       DASYSV010000573         6801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2038239, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000573 DASYSV010000000
VERSION     DASYSV010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038239"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VGQ51_17845"
     CDS             <1..619
                     /locus_tag="VGQ51_17845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9038506.1"
                     /translation="PAHKQQEIFDSFTQADADTTRKYGGTGLGLAISKRLVSLFSGEL
                     LLKSEEGKGSTFYFSLELEINENAKLYINDERVKHLPVFDSLCVLIAEDNVVNMSVAR
                     KFLTKWGIEVHEAVNGLEAVEKFRRGNFDLVLIDLEMPEMDGITALSEIRKMKPSVPA
                     IAFTAAVYEDMRIDLIKKGFMDVVPKPFRPEDLHNKIQKLLVNRA"
     gene            736..1290
                     /locus_tag="VGQ51_17850"
     CDS             736..1290
                     /locus_tag="VGQ51_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038507.1"
                     /translation="MEPPSWSGQFSRLRQLFWFFFLLTIAYMIWVRNYLAPLSSDELI
                     QFEIAKTADKAHAIIDHWKITGKYEQGVKSTNFAYIFMFLYTVAIMLGCRFISACTGN
                     EIMIKGGRGFAWLILGATVSDLIENIALSHTIRGPISQWNVTVAYDMARVKFSIVIVC
                     ILFMLACTLFWGIGKLAGEKKYSK"
     gene            1657..2163
                     /locus_tag="VGQ51_17855"
     CDS             1657..2163
                     /locus_tag="VGQ51_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HEV9038508.1"
                     /translation="MSEGKRNWYVVYTRPRWEKKVHKLILDTGMEAYCPLNRVRKRWS
                     DRIKWVEEPLFKSYLFVSVLEEQLTDVRMIDGVLNFVYWLGKPAIVREKEIQTIRKFL
                     KDYDDVQALPVDLSANDAVTISQGALMDRKARVLKVFNNKVQVIIESIGYSLVAFIDK
                     SNLSLAGK"
     gene            2173..3033
                     /locus_tag="VGQ51_17860"
     CDS             2173..3033
                     /locus_tag="VGQ51_17860"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEV9038509.1"
                     /translation="MKISGIGLVCSTVLLTTLLGSCGNTRQLTYLQGQFDTTKLSQLP
                     VIDPTIKKGDLLSIVVYSDNPQATAIFNQSLITVPATQASGMSGGAGGTGQGGNSGGG
                     TASMTGLSNAAPAQSPGYLVDENGNISFQGLGRLHVEGLTKAQLKDTLDERLKDQLKN
                     PYYTIRFMNYRFTMLGEIQRPGVYSMPGESVSLLEALGMAGDMTFFGRRDNVLVIRSN
                     NGKREFARLDLTKPDAMTSPYFYLQQNDLIYIEANKKKVAANDQVTARNVAIGASIVS
                     TLAIVYSIFR"
     gene            3061..5493
                     /locus_tag="VGQ51_17865"
     CDS             3061..5493
                     /locus_tag="VGQ51_17865"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEV9038510.1"
                     /translation="MADTDIRTPNSFQTDGRATNMLTEGPKEFLFKYLHYLPWIILSC
                     ALFLVGAYVKIRYTTQIYSVQSSLLIKNDQENQGGQEARFDQLLMSGNNVNLKNEIEI
                     LKSRPILTRVAKDLGLETFIYNTGKVRSSLLYPEHPFNLQIYNLGDSIRGFDYNMTIL
                     DDNRYLLGQNKTPVLFGQVIMVDGNRFCLIRNKNLSLHIYSRPTFEFGWTPLSNIAAS
                     MIGSLSIGQLSDQATILSLSYETENPSLGQDVLNTVMAVYDTLIVEDKRRTTSNMLQF
                     IQDRLNEMGDTLKGVQGVLKQFIVENQAFDIEGQSKSYLDKMGESGKQDVEQQVKIKI
                     VNFLLKYIDDQKNKFELVPTNLGIEEPALQQLILEYNRLQLERDANLKTTAANNPMIQ
                     ALEGSLNKVRTNIYQALLNVRQAYEIASANLAKQNEDLQSRIKGLPGKSIQLLNFERQ
                     QKIFEDLYSLLLQKKLETSISAAATVSNSRIVEPAMSSDQPVRPNKKNIYISFFLIGL
                     AIPCGLIGMKEVLRDKVSGRADVEKFTTAPILGEIGHSDYDQPLVVTRTSRRFISEQF
                     RIIRTNLQYIISNKEKVVIMVTSSFSGEGKSFISTNMGAVMAVSGKKTVIIEFDIRKP
                     KIVSGLDLKRKMGITNYIIGKASFEELLVKVEDVDDLYVIPCGPIPPNPAELLLDRKL
                     DELMKEVMANFDVVILDTAPVGLVSDALGLARFADCTLYIIRQGHTFRKQLGFIEELY
                     VEKKLPRLCLLLNDMKPEGGYYGGYYGGGYGYYTGYSYGNNSGYFEEETNRKSQRTIF
                     GRIKRFISRIFF"
     gene            5527..6375
                     /locus_tag="VGQ51_17870"
     CDS             5527..6375
                     /locus_tag="VGQ51_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038511.1"
                     /translation="MGEERLLSELIIEPGRSTKNYWGDLWRYRELFYILSWRDIKVRY
                     KQTVIGAAWGVIRPLLTMIIFSVVFGRVAKMADPNAHYPYTLLVFAGLLPWQFFATAL
                     AESSVSLIGNANLISKVYFPRMIVPASSVITSMVDFAISFGILLVMMAFYHYLPPVQI
                     FILPFFVFLTLLLSLGTGLYVTALNVKYRDFRYIIPFIVQIGVYITPVGYSSTKITQQ
                     FSENARFWYSLNPAVGIIDGFRWCILGEPLYWPGFFLSIVVTLLFVWLGTWYFRKTEK
                     SFADNI"
     gene            6379..>6801
                     /locus_tag="VGQ51_17875"
     CDS             6379..>6801
                     /locus_tag="VGQ51_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9038512.1"
                     /translation="MKKAIIRVENLSKRFIIGHEVTERYTTLGDTFIRKTKRLLGRGD
                     QKTGTVKEEFWALKDVSFEVGEGERIGIIGRNGAGKSTLLKLLGRITEPSAGRITING
                     RVASLLEVGTGFHPELSGRENIYLNGAVLGMARSEIKRK"
BASE COUNT         1748 a         1822 c         1646 g         1585 t
ORIGIN      
        1 acccgcgcat aaacaacagg agatcttcga tagctttaca caggcggatg cggacacgac
       61 tcgaaaatac ggcggcaccg gtctcggcct ggccatcagc aaacgcctgg taagcctgtt
      121 ctcgggagag ctcctgctaa aaagcgaaga aggaaaaggc agtactttct atttctcgct
      181 cgagctcgag atcaacgaga acgcgaagct gtatattaat gacgagcggg ttaaacactt
      241 acccgttttc gacagtttgt gcgtgctcat cgccgaagat aatgtggtca atatgtccgt
      301 cgcccggaag ttccttacca aatggggcat cgaggtccac gaggccgtca atggtctcga
      361 agccgtggaa aaatttcgcc ggggaaactt cgatctcgtg ctgatcgatc tcgagatgcc
      421 tgagatggat ggcatcaccg cactttcgga aataagaaag atgaaaccct cggtaccggc
      481 tatcgctttt actgcggcgg tatatgagga tatgcggatc gatctgataa aaaagggttt
      541 catggacgtc gtacccaagc ccttccgccc cgaagacctt cacaataaga tccaaaaact
      601 actcgtcaac cgcgcatagc gtgcagccgc cccccggcat aacccgccgg gacgatcagg
      661 ggacgaaaaa ttatcctttc aggtactgcc gattttatct aacttaacgg tttcaagata
      721 aaatcggcat tttttatgga acctccctcg tggtccggtc agttcagccg tctgcgacaa
      781 ttattctggt ttttcttcct gcttaccatc gcctatatga tctgggtaag aaattacctc
      841 gcccccctca gctcagatga actcatacaa ttcgagatcg ccaagacggc ggacaaggcc
      901 catgctatca tcgaccactg gaaaatcacc ggcaaatacg aacaaggtgt caaaagcaca
      961 aattttgcct atatcttcat gttcctctat accgtggcca tcatgctcgg ctgccgcttc
     1021 atctccgcct gcaccggcaa tgagatcatg atcaagggcg gacgagggtt cgcctggctg
     1081 atccttggcg ctaccgtctc cgacctcatc gagaatatcg cactttccca tactatccgc
     1141 ggccccatct cccagtggaa cgtgaccgtc gcttacgata tggcccgggt taaattctcg
     1201 atcgttatcg tctgcatcct cttcatgctc gcctgtacac tcttctgggg cataggcaag
     1261 ctcgcaggag aaaagaaata ctccaaataa cccaatctta aaagctggtt ttcaatcttc
     1321 cattctcaag ctcgaatatc aaaaacactt atatttgcgg caaggtatcg gcagggccgt
     1381 tcgaaggggt tgtatgatcg ttttatctaa acaataaatc cggtgattag gagttttctg
     1441 taacccgatg gttttagata cctttatagc ttgtaattct gtgaagaatc attttaagat
     1501 tttagtgcta ttattcaatt atggaaaggg tataaggagt atttcttagg aatgctctct
     1561 tatgcccttt tcagttgaca gcaataaagt attaatccgt accttgctga taattaattc
     1621 attacaaaaa gtccatggga ctgaggtggc gaagctatgt cagaaggtaa acgaaattgg
     1681 tacgtggtct ataccaggcc ccgttgggag aaaaaggtac acaagcttat ccttgatacc
     1741 ggtatggaag cctattgccc gctgaacagg gttcgcaaaa gatggagcga ccggatcaaa
     1801 tgggtcgaag agcccctttt caaatcctac ctgttcgtgt cggtcctgga agaacagctg
     1861 accgatgtcc ggatgatcga cggagtcctg aatttcgtct attggcttgg caaaccggcc
     1921 atcgtcaggg aaaaagagat ccagaccatc cgcaagttcc tcaaagacta tgacgacgtc
     1981 caggccctcc ccgtcgacct gtccgccaac gatgcggtga ccatcagcca gggagcgctc
     2041 atggacagaa aagcaagggt actgaaggtg ttcaacaata aggtccaggt gatcatcgaa
     2101 agcatcggtt attcactggt ggccttcatc gacaaatcca acctgtcatt ggcaggtaaa
     2161 tagataaaaa acatgaagat atccggaatc ggtcttgtgt gttccacggt gctcctgacg
     2221 acattgctcg gatcctgcgg caataccagg cagctgacct atttgcaggg ccagttcgat
     2281 acgacaaagc ttagccagct gccggtgatc gatcccacca tcaagaaagg cgatctgctc
     2341 agcatcgtcg tttatagcga caacccccag gccaccgcca tcttcaacca gtcgctgata
     2401 accgttccgg cgacgcaggc ctccggtatg tccgggggcg ccggaggcac cggtcagggt
     2461 gggaattccg gcggcggcac cgcttcgatg accggcctta gcaacgcggc tcctgcgcaa
     2521 tctcccggct atctcgtcga tgagaatggt aatatttcct tccaggggct gggtaggttg
     2581 catgtcgaag gccttaccaa ggcccagctg aaagacacac tcgatgaaag actgaaagat
     2641 cagctgaaga acccctatta tacgatccgg ttcatgaact accggttcac gatgctcggc
     2701 gagatccaga ggccgggcgt ttatagcatg cccggtgaaa gcgtcagcct gctggaagcc
     2761 cttggcatgg ccggcgatat gacttttttc ggcagacgcg acaatgtcct cgttatccgg
     2821 tccaacaatg gcaaaaggga attcgcgaga ctggatctta caaaacccga tgccatgacc
     2881 tccccttatt tttatcttca acagaacgat ctgatctaca tcgaggcgaa caagaaaaag
     2941 gtcgcggcca acgaccaggt aaccgcccgc aatgtcgcta tcggtgccag tatcgtttcg
     3001 acgctggcga tcgtttacag cattttcaga taacccttaa tacttaatat ttaatatcct
     3061 atggccgata ccgatatacg aacaccgaat tccttccaga ccgacggcag ggccaccaac
     3121 atgctgaccg aaggaccgaa agaattcctt ttcaaatacc tccactatct cccgtggatc
     3181 atactatcct gcgcgctctt cctcgtcggg gcgtatgtga agatcaggta taccacccag
     3241 atctattcgg tgcagtcttc actcctcatc aaaaatgacc aggaaaacca gggcggacag
     3301 gaggcacgtt tcgaccagct gctgatgtcg ggcaacaatg tcaatctcaa gaacgagatc
     3361 gagatcctga aatcacggcc gatcctgaca cgcgtggcga aagacctggg tctcgagact
     3421 ttcatttaca acacgggaaa ggtccggtcc agccttctct atcctgaaca cccgttcaat
     3481 ctccagatat acaacctggg cgattctatc agggggttcg attacaatat gaccatcctg
     3541 gacgataacc gttatctgct cggccagaat aaaacgcccg tactcttcgg tcaggtgatc
     3601 atggtggacg ggaaccggtt ctgtctcatc cgaaacaaga acctgtcact acatatttat
     3661 agccggccta ccttcgaatt cggctggacg cctctttcca acatcgcggc ctccatgatc
     3721 ggctcgcttt ccatcggcca gctcagcgat caggccacca tcctctctct ttcctacgag
     3781 accgaaaatc cttctcttgg ccaggatgtc ctcaatacgg tgatggccgt atatgatacc
     3841 ctcatcgtag aagacaaacg gcgtacgact tccaatatgc ttcaattcat tcaggaccgc
     3901 ctgaacgaaa tgggcgatac cctcaagggc gtacagggcg ttctgaagca atttatcgtc
     3961 gagaaccagg cgttcgatat cgaaggtcaa tcgaaatcct atctcgataa aatgggcgaa
     4021 agcggcaaac aggacgttga acaacaggtg aagatcaaga tcgtcaactt tctgctgaag
     4081 tatatcgatg atcagaagaa caagttcgag ctggtgccga ccaacctggg tatcgaagaa
     4141 cctgcgctcc agcagctgat cctggagtac aaccggctcc agctggaacg cgatgccaat
     4201 ctgaaaacga ccgcagccaa caaccctatg atccaggccc tcgaagggtc actgaacaag
     4261 gtccggacaa atatctacca ggccctgctc aacgttcgcc aggcttatga gatcgcttcc
     4321 gccaacttag ccaaacagaa cgaggacctg caaagccgca tcaagggact ccccggtaag
     4381 tccatacagc tcctcaactt cgaaagacaa cagaagatat tcgaagacct ctattcgctc
     4441 ctgcttcaga aaaagctgga aacctctatt tccgcggcgg caaccgtatc caattcccgg
     4501 atcgtggaac cggccatgag ctcggaccag cccgtccggc cgaataaaaa gaatatctat
     4561 atctccttct tcctgatcgg tctggccata ccctgtggcc tcatcggtat gaaggaggtg
     4621 ctgagggata aggtcagcgg ccgggccgat gtggaaaaat ttacgacggc acccattctt
     4681 ggagagatcg gccattccga ttacgatcaa cccctcgtgg tcacccgcac cagccgcaga
     4741 ttcatctccg aacagttccg tatcatccgg accaatctcc aatacatcat ctccaataag
     4801 gaaaaggtcg tgatcatggt cacctcctcc ttcagcgggg aaggaaaatc ctttatcagc
     4861 accaatatgg gcgccgtaat ggcggtttcc ggtaaaaaga ccgtcatcat agagttcgat
     4921 atccgcaagc cgaagatcgt ttcgggtctc gatctgaaac gaaaaatggg cattacgaac
     4981 tacatcatcg ggaaagcctc cttcgaagag ctgctggtta aagttgagga tgtcgatgat
     5041 ctctatgtca ttccctgcgg cccgataccg cccaacccgg ccgagctgct tctggacaga
     5101 aagctcgatg agctgatgaa ggaggtcatg gccaatttcg atgtggtgat cctcgacacc
     5161 gctcctgtag gactcgtcag cgatgcgttg ggtctggcaa gattcgcgga ctgtaccctg
     5221 tacatcatcc gtcagggaca caccttccgg aaacagctgg gattcatcga ggaactgtac
     5281 gtcgaaaaga aactgccgcg gctctgtctg ctgctcaatg acatgaaacc ggaaggcggg
     5341 tattacggcg gatattatgg aggaggttac ggttattaca ccggctacag ctacggcaac
     5401 aattccggct actttgaaga ggagaccaat agaaaaagcc agcggacaat tttcggccgt
     5461 atcaagcgtt tcatcagtcg tatttttttc tgatctaaat cttagcgtac aaatctatta
     5521 atatccgtgg gagaagagag actgctttca gaactgatca tcgaacccgg tcgcagcacc
     5581 aaaaattatt ggggcgatct atggcgctac cgggagcttt tctatatcct tagctggcgg
     5641 gacatcaagg ttcggtacaa acagaccgtc atcggtgccg catggggagt tatccggccg
     5701 ctgctgacca tgatcatttt cagcgtggtc ttcggtcgcg tggcaaaaat ggccgacccc
     5761 aatgcccact atccctatac ccttcttgta ttcgccggtt tgctaccctg gcaattcttc
     5821 gccaccgccc tggccgaatc cagcgtcagc ctcatcggca atgccaacct gatctcgaag
     5881 gtctatttcc ccaggatgat cgtccccgcc tcgtcggtca tcacgagcat ggtagacttc
     5941 gcgatatcat tcggcatcct gctggtcatg atggcctttt atcactattt gccacccgta
     6001 caaatattca ttctcccctt cttcgtattt ctcaccttat tgctttctct cggcaccggg
     6061 ctgtatgtca cggcattgaa cgtgaaatac cgcgacttcc ggtacattat acccttcatt
     6121 gtgcagatcg gagtatacat cactccagta ggttattcga gcaccaagat aacgcagcaa
     6181 ttctccgaga atgcccgttt ctggtattcg ctgaatcccg cagtgggcat catcgacggt
     6241 ttccgatggt gcattctggg tgaacccctg tattggccgg ggttcttttt atccatcgtg
     6301 gtcaccttat tattcgtctg gcttggaaca tggtatttcc ggaagaccga aaagagtttt
     6361 gcagataaca tctaagacat gaagaaagcg attatcaggg tcgaaaacct gtcaaagcga
     6421 ttcattatcg gtcacgaagt aactgagcgc tatactacac tcggcgatac ctttatcagg
     6481 aagaccaaaa ggcttcttgg acggggagac caaaagacgg gcacggtgaa ggaggaattc
     6541 tgggcgctga aagatgtcag ctttgaagtg ggtgaaggtg aaaggatcgg gatcatcggc
     6601 cggaacggag cgggcaaatc cactctcctc aagctcctcg gtcgtatcac cgaaccttcg
     6661 gcgggccgaa tcactatcaa cggacgcgtc gcctcgctgc tggaagtagg aacgggtttc
     6721 caccccgagc tctcgggcag ggagaatatc tatctgaacg gagccgtcct cggcatggcc
     6781 agatccgaga tcaaaaggaa a
//
LOCUS       DASYSV010000574         6101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1524338, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000574 DASYSV010000000
VERSION     DASYSV010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524338"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VGQ51_17880"
     CDS             complement(<1..1104)
                     /locus_tag="VGQ51_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038513.1"
                     /translation="MSSSTLPSSLRPASASAKYTVSIAAFLALLIPLAFMVGKVLEHT
                     HGVFAYPLDDSYIHLAVAKNLADHGVWGVSAHEFASASSSILYPLLLAGIFKITGVLT
                     IMPFLVNIVVALIFLLVVQRWLVRQGVAPMGQLIVLLLAVFLIPLPGIVMVGMEHTLH
                     LLFFFLFLTSFAEALEASLASPSSRPVIPWPVYLYGVLMMAARFESMALLGFACLLLL
                     YHRRWMAAVSLGFVCFLPVLIFGVISLSKGGFFLPNAVMLKPSLPHGGLDEILYYVGQ
                     EYIPRFFSSSERYNTMGVQRLVLLIPLAYFLFRRATREKIAYRYMLFMLLAATVFHLA
                     FAAYSFYARYEVYLVGSFVVVVATLFVKYFRVEP"
     gene            complement(1245..3881)
                     /locus_tag="VGQ51_17885"
     CDS             complement(1245..3881)
                     /locus_tag="VGQ51_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HEV9038514.1"
                     /translation="MKSIFFGLLLLGAVPSVFGQTGGADPVGGNGAAAPVAGNGAVAP
                     VRGNGAAAPAGGNGAPAPAGGNGGSAPAGGHSFNGVYTGSNLDMVSFPIGGIGAGMFC
                     LDGTGTISHLSIRNEPAVFNTPKAFAAIGVKGDPDGARVLEGPVPGWKRFSVPSGGHG
                     VYGTDIGLARFREAVFLGRFPFAEIRLHDSVLPLDVQITGWSPFIPTDADNSSLPVGA
                     LEYHFKNTSARSREYVFSFNSATKRDFSQVVPMEHGFILSQDTARAPEAKGDLAFFTD
                     DPATVVDYCWFRGGYYDPLTITWKHIRDASAVAVDPQPGGRGASLFVPFVLGAGEEKT
                     IRVYVAWYVPDTRLRFGSHFTDPADSTVRDASVDLPSPYHKPWYSSKFGSIGEVTAYW
                     LTHYASLRQESVAFRDAFFRSTLPPEVTEAIAANLSILKSPTVLRQYDGRFWAWEGCE
                     DDEGSCPGSCTHVWNYAQALPHLFPVLERSMRQTEFFEDQNAAGHQQFRAALPIRPQK
                     HDFYAASDGQLGGIMKVYRDWRICGDSLWLRGLYPRVKASLDYCIRTWDPRHKGTLEE
                     PHHNTYDIEFWGPDGMTTSVYAGALEAFVAISDYLGEDAADYSALYARCRRYMEDQLF
                     NGAYFIQKIQWKGLNAADPVAFSEHSIHSGYSPEALELLKREGPKYQYGSGCLSDGVI
                     GDWMARVSGLPGPLDSSKVSSHLLSVYKFNLVKDLSGHSNPERPGYALGAEGGLLLCT
                     WPQGGQLSLPFSFAPEVWTGVEYEVASHLIFQGRVREGLDIVRTARSRYDGRVRDPFD
                     EYECGHWYARALSSYALIEALTGVRYDAVTQTLYVDSKVGDFVSFLSTATGFGNVVYR
                     GGKARVEVVHGAIAVREIHYGR"
     gene            4175..4864
                     /gene="trmD"
                     /locus_tag="VGQ51_17890"
     CDS             4175..4864
                     /gene="trmD"
                     /locus_tag="VGQ51_17890"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV9038515.1"
                     /translation="MSLNIDILSVLPELLESPFNHSIIRRAKEKGLLEINTHHLRKWA
                     VNEYGQIDDYQYGGGAGMVMMPEPLAAAIDSLSAPKPYDEIIYFTPDGTTLDQRMANR
                     LSLKSNLLLICGHYKGIDQRIRDRYVTMEISIGDYVLSGGELAAAILVDAIGRLIPGV
                     LNDETSALFDSFQDNLLAPPVYTRPADWRGEKVPDVLLSGDPRKIEDWRNEQSLQRTR
                     QRRPDLLEGEE"
     gene            5374..>6101
                     /locus_tag="VGQ51_17895"
     CDS             5374..>6101
                     /locus_tag="VGQ51_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038516.1"
                     /translation="MTISGFTMVRNATKLYYPIREAISSVLPLVDEFVVAVGDCDPDD
                     RTLKEIAAIGSDKIRIIHTVWDLDKYPRGMEHAHQTDIAKAACTGDWLFYIQSDEVVH
                     EDDLPVIRARCAELLEDRSVEGILFKYRHFWGDYDHYITSHAWYANEIRIIRNDRDIH
                     SWESAQSFRRIPGFDGIHYRQKKGTYKLKVARVDAHIYHYGWVRPPEVMLRKQRSFER
                     LYHDERWLGRHGLREDFDPNRIYE"
BASE COUNT         1589 a         1877 c         1474 g         1161 t
ORIGIN      
        1 cggctcgacc cggaaatatt tcacgaacaa cgtcgcgacg actacgacaa acgaccctac
       61 gagatacacc tcataccgcg cataaaatga ataggccgca aaggcgaggt gaaagaccgt
      121 tgccgccagc agcatgaata acatataccg atacgcgatc ttttcgcgtg tcgcgcgtct
      181 gaacagaaaa taggccagtg gtatcagcaa caccagccgc tgtacaccca tggtattata
      241 tcgctcgctc gacgaaaaaa atcttggaat atattcctgt cccacataat acaatatctc
      301 atccagccct ccatgcggca gcgacggctt cagcatcacc gcattcggca gaaagaaccc
      361 gcccttcgac agcgagatca ccccgaagat cagcaccggc agaaaacaaa cgaaaccaag
      421 agacacagcc gccatccacc gacggtgata caaaagcagc agacaggcaa accccagcaa
      481 ggccatgctt tcaaaacgcg ccgccatcat cagcacccca tatagataca caggccacgg
      541 tattaccggc cgcgacgaag gcgacgccaa actggcttcc agcgcctccg caaaggaggt
      601 cagaaaaaga aagaagaaca aaagatgcag cgtatgttcc atccccacca tcacgatccc
      661 cggcaaaggg attaaaaaca cagccaacag caaaacgatc aattgcccca tcggtgcaac
      721 gccctggcgg acaagccagc gttgtaccac cagcaagaat atcagtgcga ctacgatgtt
      781 caccaggaaa ggcatgatcg tcaatacccc cgtgatctta aaaatgccgg ccagcagcag
      841 cggatagagg atagaggaag aggccgaagc gaactcatgt gccgacacac cccaaacgcc
      901 atgatcggcc aggttcttgg ccaccgccag atggatatag ctgtcatcca atggatatgc
      961 aaagaccccg tgggtatgtt cgagcacttt ccctaccata aaggccagcg ggatcaacag
     1021 cgcaagaaat gcagcaatac taacagtata tttggcggaa gcagaagccg gccgcagcga
     1081 cgatggtaga gtagacgagc tcatgacacg aatctattcc ttccccccgc aattcccttt
     1141 cagtaaacaa aaacctcgcc gcacccgcac accgcacgcc gctcaccgcc taccgcaccc
     1201 gcccatctca ccgcacaccg cacccggcca cctcaccgcc cacctcaccg cccataatgt
     1261 atctcccgca ccgcaatcgc tccatgcacc acctccaccc tcgccttccc cccgcgatac
     1321 acgacattcc caaaccccgt agccgtcgac aaaaaactca caaaatcccc caccttcgaa
     1381 tccacataca aagtttgagt caccgcgtca tatcgaaccc ccgtcaatgc ctcgatcaac
     1441 gcatagctgc tcaatgccct ggcataccag tgaccgcatt catattcatc gaaaggatcg
     1501 cgtacccggc cgtcataccg gctgcgcgca gtgcgaacaa tatcgagtcc ctcgcgcacc
     1561 cgaccctgaa agatcaaatg cgaagcgacc tcatattcga cacccgtcca tacctccggg
     1621 gcgaagctaa aaggcagtga cagctgtccg ccctgcggcc acgtacacag caaaagcccg
     1681 ccctcggccc ccagtgcata tcccggtcgt tccgggttac tgtgacccga caggtccttc
     1741 acaagattga acttatacac cgacaataag tgcgaactca ccttcgaaga atccagcgga
     1801 cccggcaacc cgctaacacg cgccatccaa tccccgatca ctccatccga aagacagcct
     1861 gaaccatact gatacttcgg cccttcccgc ttcagcagct ccagggcttc aggtgaataa
     1921 ccactatgga tcgaatgttc cgaaaaagca accggatcgg cggcattcag tcccttccat
     1981 tgtatcttct gaataaaata ggctccattg aacaactgat cttccatata ccgccggcaa
     2041 cgcgcataca gcgccgaata atccgccgca tcctcgccca gatagtcgga gatagctacg
     2101 aaagcctcca gcgcacccgc atatacactc gtcgtcatcc cgtcaggtcc ccagaattcg
     2161 atatcatagg tgttatggtg aggctcttcc agcgtgcctt tatgacgcgg gtcccatgta
     2221 cggatacaat agtcgagact cgccttcacc cgcggataca acccccgcaa ccacaaacta
     2281 tccccgcaga tccgccagtc gcgatatacc ttcatgatcc cgccgagctg tccgtccgac
     2341 gctgcgtaga agtcgtgctt ttgcgggcgg atcggaagtg cggcgcgaaa ctgctgatga
     2401 cctgccgcgt tctgatcctc aaaaaactcc gtctgccgca tactgcgttc caaaacagga
     2461 aataaatgcg gaagcgcctg cgcatagttc cagacatgtg tacagcttcc cgggcaggag
     2521 ccttcatcgt cttcgcatcc ttcccatgcc cagaacctac cgtcatattg acgtaatacc
     2581 gtcggagact tgagtatcga caaattagcc gcgatggcct ccgtcacctc cggcggcagc
     2641 gtgcttcgga agaaggcatc gcgaaaggcg accgactcct gacgcaacga tgcataatgc
     2701 gtcagccaat atgccgtcac ctcgccgatg gaaccaaact tactgctata ccatggctta
     2761 tggtagggtg aaggaagatc caccgacgca tcgcggaccg tactatccgc cggatcggtg
     2821 aaatgcgagc caaaccgtaa ccgggtatcc ggcacatacc acgctacata tacccggata
     2881 gtcttttcct cccccgcccc cagcacaaac ggcacaaata acgatgcgcc cctacccccg
     2941 ggttgcggat ccaccgccac cgccgatgca tcccgtatat gcttccacgt tatggtcagc
     3001 ggatcgtaat agccaccgcg gaaccagcag tagtcgacca ctgtcgcggg gtcgtcggta
     3061 aaaaaggcca gatcgccctt cgcctccggc gcccgggcgg tatcctgcga gaggataaac
     3121 ccgtgttcca tcggcaccac ctgtgaaaaa tcgcgtttgg tagcggagtt gaaggaaaag
     3181 acatattcgc gggagcgcgc cgaggtattc ttgaaatgat attccagcgc gcccaccggc
     3241 agcgaagaat tgtcggcatc cgtgggaata aaggggctcc agcccgtgat ctgcacatcc
     3301 agcggcagca cggaatcgtg cagccggatc tcggcgaaag gaaatcgtcc caggaaaaca
     3361 gcctcgcgaa aacgcgccag gcctatgtcg gtgccgtata ccccatggcc cccggaagga
     3421 acgctaaacc ttttccatcc tggaaccggc ccttcgagta cccgtgcgcc gtcgggatcc
     3481 ccttttaccc ctattgcggc aaaagccttg ggagtgttaa ataccgccgg ttcattgcgg
     3541 atcgacagat gcgatatcgt tcccgtcccg tccaggcaga acataccggc gccgatgcca
     3601 ccgatcggaa atgaaaccat atcgagattg gaacctgtat aaacgccgtt gaaggagtgg
     3661 ccgcccgcgg gagccgaacc accattgccg cccgcaggag ccggtgcccc attgccgccc
     3721 gcgggagccg ccgcaccatt cccacgcaca ggagccaccg caccatttcc ggccacagga
     3781 gccgctgcgc catttccccc cacaggatcc gccccacccg tctgcccaaa aacactgggt
     3841 accgcaccca gcagcaatag cccgaagaat atcgatttca tgtcacaaca acggcaaaac
     3901 gactgaacaa ccactgcagc agtaaaaatt aaccccggcg cccgccgtcc ctaaaaacga
     3961 ctcggcccgc ttcccgggta ccggagcgag ccgaaatcta gaggtttaat ggatatggac
     4021 cgattaacgc aacccacttg tacttgaggt tgattggtcg agcatatgtg acagcaggga
     4081 aaattcaagg gttctataag ggaacgatgc ttagtacaaa aaggatgcct gttccggtgt
     4141 taaaaagatt gcagtttttg agtaggtttg cagcatgagt cttaacatag acatcctcag
     4201 cgtattaccc gaattgctgg aatctccgtt caaccattcg atcattcgcc gggccaaaga
     4261 aaaaggctta ttggagatca acacccatca tctaaggaag tgggccgtca atgaatacgg
     4321 tcagatcgac gactaccagt atggcggcgg agcggggatg gtgatgatgc cggaaccgct
     4381 ggcggccgcg atcgactcgc tgtcggcgcc caaaccttac gatgagatca tctatttcac
     4441 cccggacggc acgacgctcg accagcggat ggccaaccgg ctttcgctga aaagcaatct
     4501 cttgctgatc tgcgggcact ataagggtat cgatcagcgg atacgcgatc gttatgtgac
     4561 gatggaaata tctatcggag actatgtcct cagcggtggc gagctggcgg cggccatcct
     4621 cgtggatgcc atcggaagac tgataccggg ggtgttgaat gacgagacat cggcgttatt
     4681 cgactctttc caggacaatc tgctggcgcc gcccgtatat acgcggcccg ccgattggag
     4741 gggcgagaag gtgccggacg tgctgctgag cggcgacccc cgaaagatcg aggactggcg
     4801 taatgaacaa tccctccaac ggaccaggca gcgaaggccg gatctattgg agggagagga
     4861 ataaaaagtc tgctccgcga gccgccaccg gcggctcagg aaccccaggg ttcattctag
     4921 cgtaatacca tcgaagccca tatcagcaat tgcaaaatca ggtaatagcc aaggatcagc
     4981 agcgtgttgt agacgatcgt ctttctcata aaggaaacgg atttaaaatt tgcctccagg
     5041 cccgcccctt gaagaccaaa atagtaccaa actccgcctt ttcaaatagg atttctacca
     5101 gcaaagccct gaaaattccc cgcgaaaaag agctctcaac tccctttatt tttttaatgt
     5161 cattttaatt cacgcttaac cggtgatttt ccgctcaata gcgcaaacct accggttatc
     5221 caattgttaa accgtagcct cgtatacaaa cacccactta tcagcgttta atcttctctt
     5281 aattttttta gtccaaccct gcacggaaat tgcacgtcta tagataagca gaaagacgta
     5341 aaactgtcca taggatcaca aaccaactcg taaatgacta ttagcggatt taccatggtg
     5401 cggaatgcca ccaaactcta ttatcctatc agggaggcta tctcatccgt gttgcccctc
     5461 gtcgatgagt tcgtcgttgc ggtgggcgac tgcgatcccg acgaccggac attgaaagag
     5521 atcgctgcca tcggctctga taagatccgt atcatccata ccgtttggga tctcgacaag
     5581 tacccgcggg ggatggagca tgcgcatcag accgacatcg cgaaggcggc ctgcaccggc
     5641 gactggctct tctacatcca gagcgacgag gtcgttcatg aggacgatct gccggtcatc
     5701 cgggcccgtt gtgcagagtt gctcgaggat cgttccgtag agggcatcct cttcaaatac
     5761 cgccactttt ggggagatta cgatcactat atcacctcgc atgcctggta tgccaatgag
     5821 atcaggatca tccgtaatga cagggatatt cactcctggg aatcagcaca atcttttcgg
     5881 cgcatcccgg gtttcgacgg cattcactat cgccagaaaa agggtaccta caagctgaag
     5941 gtagcgcgcg tggatgcgca tatctatcac tacggctggg tgcggccgcc cgaggtgatg
     6001 ctgcgcaagc agcgcagctt cgagcggctg tatcacgacg aacgctggct cggccggcac
     6061 ggcttgcgcg aagacttcga tccgaaccgc atttacgaga a
//
LOCUS       DASYSV010000575         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1524344, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000575 DASYSV010000000
VERSION     DASYSV010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524344"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VGQ51_17900"
     CDS             <1..694
                     /locus_tag="VGQ51_17900"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEV9038517.1"
                     /translation="ENSLSGVLNDEAYGVNGLEYDVQLTKDHVPITMHDGNIDINLTQ
                     KSPLSGSWDQYTFAFLEGYIRLLNGEKIPSLDQCLTAFIDSTNMKYFWMDIKGDDSIF
                     QALMPVIQKAEAHAKAVGRDVVIYADIPSTDVIAEFQSYPPYRAAGLKTMCELELQDV
                     MDNGSTYWGPRYSRGLLLDDVEKAHAQGIKVISWTLNDKDLIANYLQNGKFDGFISDY
                     PAYVVYDFYTTK"
     gene            705..1259
                     /locus_tag="VGQ51_17905"
     CDS             705..1259
                     /locus_tag="VGQ51_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038518.1"
                     /translation="MVAAIWLMGHRLEAQPAAYHKFVVYAGAGPGYFFNNLVVAKDQV
                     RPWQYAFAARVMWEPEHSDLSLGIQTGYYRLYGVKVTQPVDAHITNSSVPILFVIAMR
                     FPKGFYGQWAMGQSITFNHVDAQGVEGNHDAKTWSLADFEATLGYRILAKSRISYAVE
                     LKGFYSTAYDNKTLALLFVVGYRL"
     gene            complement(1233..2198)
                     /locus_tag="VGQ51_17910"
     CDS             complement(1233..2198)
                     /locus_tag="VGQ51_17910"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV9038519.1"
                     /translation="MAARQKIFTRLIFAAALFCNHAADGQTSPAIHPSATEIAFVSDT
                     QQPMWEEELFLRSDHNTKATALIFSDILRQKPTALYLLGDIVALGYKRSKWVTVDSFL
                     SKCHREGIRTFGLLGNHDVMGEPKKGTRNFQKRFPDHVYTGYLSIIDSIAIVLLNSNF
                     SKLSSADQRRQQAWYDSTLRNLDSAPRIKGIIVACHHAPYSNSRIVGSSQPVQERFVP
                     AFMNTGKALLFITGHSHNFEYFKIREKTFLVIGGGGGLHQPLDTSQRRRPDLAATYKP
                     EFHYLKLDIFPDRLRLTSRSLNKDFRGFTDSFSFDIGLQPVSNHK"
     gene            complement(2221..3546)
                     /locus_tag="VGQ51_17915"
     CDS             complement(2221..3546)
                     /locus_tag="VGQ51_17915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEV9038520.1"
                     /translation="MPLVDLVMPKMGESIMEATILKWHKHPGDAVKQDETVLEIATDK
                     VDSEVPSVTEGIVEEVLYKVNDVVPIGSVIARIRTNGASTSPSASYAATPVQAAPASY
                     RPETTGSPRPANGTARFYSPLVLNIAAGEGIPLSELESIPGSGNEGRVTKKDILQYLA
                     DRRSGHGSPAPAVTTRYQGTEGTTHRLPEPSQQLPAQQTPMPVYSGNVEIIEMDRMRR
                     LIADHMVRSVHTSPHVTSFSEADVTHLVHWRDKVKKDFEKRENTKLTFTPLFIEAVVR
                     CIKKYPLINSSVEGDKIIIKKDINIGMATALPSGNLIVPVIQHADQLNLTGLARQVNT
                     LADNARQNKLRPEDTQGGTFTLTNVGSFGSLMGTPIINQPQVAILAVGAIKKRPVVLE
                     TPEGDAIVIRHMMYLSLSYDHRIVDGSLGATFCSAVARELENFSPNREL"
     gene            complement(3583..>4192)
                     /locus_tag="VGQ51_17920"
     CDS             complement(3583..>4192)
                     /locus_tag="VGQ51_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEV9038521.1"
                     /translation="VRLRLTARGEDHATTEPLLQQHFHRLKTLVSEWMVTDEDISIQE
                     AVGRLLLSGGKTLSTAESCTGGYIAHLITSIPGSSRYYKGSIVSYDNAVKMDVLHVGM
                     PTLAHDGAVSEATVREMVSGALSVLDTDYAIATSGIMGPDGGTPEKPVGTVWIAVGNH
                     QNILTQKMAFRFDRTRNIELTATAALNLLRKFIRPTEPTPTS"
BASE COUNT          893 a          970 c         1290 g         1039 t
ORIGIN      
        1 ggaaaactct ctcagcggcg tattgaacga tgaggcgtat ggggtcaacg ggttggaata
       61 cgatgtccaa ctgacgaagg atcacgtgcc gatcaccatg cacgatggaa atatcgatat
      121 caatcttaca cagaaaagcc cgctatcggg cagttgggat cagtatacgt tcgccttcct
      181 cgaggggtat attcgtctgc tgaatggcga aaagatacca tccctggatc agtgtctgac
      241 cgcctttatc gactccacca atatgaaata cttctggatg gacatcaaag gcgacgatag
      301 catttttcag gccttgatgc cggtgataca gaaagcggag gctcatgcaa aagcggtggg
      361 cagggacgtc gttatttatg cagatattcc ttcgacggat gtcatcgctg aattccagtc
      421 atacccgcct tatcgtgcgg ccgggttgaa gacgatgtgt gaattggaac tgcaggatgt
      481 gatggacaat ggcagcacgt attgggggcc gcggtattcg agggggttgt tgttggatga
      541 cgtggaaaag gcgcatgcgc agggtatcaa agtgatcagt tggaccttga acgataagga
      601 cctgatcgcc aattatcttc aaaacgggaa gttcgacggg tttatttcgg actatccggc
      661 atatgtggtt tatgattttt atacgacgaa gtaaaatgat cgcgatggtg gcggcgatat
      721 ggctgatggg gcaccgattg gaggcccagc cggctgccta tcacaaattc gttgtttatg
      781 cgggtgctgg tcccggttat ttttttaata acctggtcgt cgccaaggac caggtgcgtc
      841 cgtggcaata tgcgtttgct gcccgggtga tgtgggagcc ggagcattcg gatctgagtc
      901 tgggaattca aacaggctat tacagattgt atggtgtcaa ggttacccag cccgtggatg
      961 cccatattac caactcatcc gtgccgatct tattcgtaat tgccatgaga ttccctaaag
     1021 ggttctacgg gcaatgggcg atgggacaat ccatcacttt taaccacgtg gatgcgcagg
     1081 gtgtggaggg gaatcacgat gcgaagacct ggtcactggc ggattttgaa gctacgctcg
     1141 ggtatcgcat tcttgcaaag tccagaatct catatgccgt cgagctaaaa ggattttatt
     1201 ctactgcgta tgacaataag acattggcgt tgttatttgt ggttggatac cggttgtaag
     1261 ccgatatcaa aagagaagct atcggtgaac ccgcgaaaat ccttgttgag acttctggag
     1321 gtgagccgga gacggtcggg aaagatatca agcttcaggt aatgaaattc cggtttatat
     1381 gttgcagcga gatccggacg gcgccgctgg ctggtgtcca gcggctgatg caacccgccg
     1441 ccgccgccga tgacaagaaa ggtcttctcc ctgatcttaa aatactcgaa attgtgagaa
     1501 tgccccgtga taaacagcag ggctttcccc gtgttcatga atgccgggac gaaacgttcc
     1561 tgtaccggtt gcgacgatcc gacgatcctg ctattggagt agggggcatg gtgacaggcg
     1621 acaataatac cttttatgcg tggagcggaa tcgaggttcc ttaaggtgga atcataccag
     1681 gcctgctgtc tcctttggtc ggctgaggat agtttgctga agttcgagtt cagcaggaca
     1741 atagctatgg agtcgataat ggaaagatac ccggtgtaga catggtcggg aaaccttttc
     1801 tggaaattcc ttgttccctt cttcggttca cccattacat catggttccc cagcagccca
     1861 aacgtccgga tgccttccct gtggcatttg gagaggaaag aatctactgt gacccacttg
     1921 cttcttttgt aaccgagggc gacgatgtct cccagcaggt acagggccgt cggtttttga
     1981 cgcaggatgt cggaaaagat cagcgccgtc gccttcgtat tgtgatcgga acgcagaaaa
     2041 agctcttctt cccacatcgg ttgttgcgta tcgctgacaa aggctatctc tgtggctgag
     2101 gggtggatgg cggggctcgt ctgtccgtcc gcggcgtgat tgcagaacag agcggcagca
     2161 aaaatcagtc gggtgaatat cttttgccgg gcggccattt tgcgttggta tggatggtgg
     2221 ctatagctcg cggttcgggg agaaattctc cagctcgcgg gcgacggcgg aacagaaggt
     2281 ggcgcccaga ctaccgtcta cgatgcggtg atcgtaagac agcgacaggt acatcatatg
     2341 ccggatgacg atggcgtcgc cttccggcgt ctccagcacg accggccgct tcttgatggc
     2401 gcctacggca agtatggcga cctgtggctg gttgatgatg ggggtgccca tcaggctgcc
     2461 gaagctgccg acattggtga gggtaaaggt accgccctgg gtgtcctcgg gtctgagctt
     2521 gttttgcctg gcattatcgg caagggtatt gacctggcgg gcgagaccgg tcaggttcag
     2581 ctgatcggca tgctggatca cagggacgat gaggttaccg gaaggcagcg cagtagccat
     2641 gccgatattg atatctttct taataatgat cttgtcgccc tcgaccgaac tgttgatgag
     2701 ggggtatttc ttaatgcaac gcacaaccgc ttcgatgaac agcggggtga aggtcagctt
     2761 ggtattctcg cgtttttcga aatccttttt taccttatcg cgccagtgga ccaggtgggt
     2821 cacgtcggcc tcggaaaaac tggtaacgtg gggactggtg tgtacgctgc gcaccatgtg
     2881 gtcggcgatg agacggcgca tacggtccat ttcgatgatc tcgacgttgc cactataaac
     2941 gggcataggt gtttgctgtg cgggaagctg ctgcgagggt tccggcaacc ggtgggtggt
     3001 gccttcggtg ccctggtacc gggtggtgac tgcgggggcg ggagagccat ggcctgaacg
     3061 acggtctgcg aggtactgaa ggatgtcctt tttagtaacg cgtccctcgt taccggagcc
     3121 ggggatggat tccagctcgc tgagcgggat gccctcgcct gcggctatat tgagcaccag
     3181 aggggagtag aatctggccg tgccattggc cggtctcggg cttccggtcg tctcaggccg
     3241 gtaggatgcg ggtgcagctt gtacgggggt ggcggcatag gaggcagagg gtgaggtgga
     3301 tgcgccgttg gtccggatcc gggcgatcac cgaaccgatg gggaccacgt cgttgacctt
     3361 atagagcact tcctcgacga tgccttcggt gacggagggg acttcggaat ccaccttgtc
     3421 ggtggctatc tccaataccg tttcgtcttg ttttacggcg tcgccgggat gtttgtgcca
     3481 tttcaggatg gtggcttcca tgatactttc acccattttt ggcatgacca ggtcaacaag
     3541 cggcatacgt tagatttaat tgaaaaccaa aggtaatata ttttatgatg ttggagttgg
     3601 ttcggtcggg cggatgaatt ttctcaatag attaagagcg gcggttgccg ttaattcaat
     3661 atttcgtgtc cggtcgaaac gaaatgccat cttttgggta agaatatttt gatggtttcc
     3721 aacggcgatc cagaccgttc cgactggttt ttcgggtgtg ccgccatcgg gccccatgat
     3781 gcccgacgta gctatcgcat agtcggtgtc gagtacggaa agggcgccgc tgaccatctc
     3841 gcggacggtg gcttcgctga cagctccatc atgtgccagg gtcggcatgc cgacgtggag
     3901 cacgtccatc tttacggcat tgtcatagct gacgatgctg cctttataat agcggctgga
     3961 gccgggaatg gaagtaataa ggtgggcgat atagccgccg gtacagcttt ccgccgtgct
     4021 taaggttttg ccgccgctga gcagcaggcg accgacggct tcctggatag agatgtcttc
     4081 gtcggtgacc atccattcgg agacgagggt cttgagccgg tggaaatgtt gttggagtag
     4141 cggttctgtc gtagcgtggt cttcgccgcg ggcggtcaga cggagacgga cc
//
LOCUS       DASYSV010000576        11519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1399942, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000576 DASYSV010000000
VERSION     DASYSV010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399942"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /gene="hemN"
                     /locus_tag="VGQ51_17925"
     CDS             complement(<1..1218)
                     /gene="hemN"
                     /locus_tag="VGQ51_17925"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV9038522.1"
                     /translation="MEANFLEKYNQPAPRYTSYPTVPFWKDHIEGVEWRANFIGRYNE
                     SNSNEGMSLYIHLPFCESLCLYCGCTKKITTNHGVEEEYIDAVLAEWRLYRQLMGQPP
                     VIRELHLGGGTPTFFSPGNLERLVKGLLSTAVVHPDHAFSVEGHPNNTTRDHLETLAD
                     LGFNRISYGVQDLNPEVQKAINRVQPFENVRRATEEARAAGFAAVNFDLIYGLPLQTP
                     ECLRRTIEGSVSLAPDRIAFYSYAHMPRVICNQRLIDTVMLPSAREKIGLYGMGQLLL
                     TEHGYAHIGMDHFALPGDELYRAWRQARLHRNFMGYTTQKSGLLLGLGMSAISDIGTA
                     FAQNDKQLTNYYRSIAAGELPLVRGYFLTEEDVVFRRYILDIACKGATVFDPASAALL
                     KEWTLPRLKELERD"
     gene            1424..2194
                     /locus_tag="VGQ51_17930"
     CDS             1424..2194
                     /locus_tag="VGQ51_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038523.1"
                     /translation="MPQPTKFRQFLEETGTVARFTGQYFVQTGKRRFEFSELLRQCYL
                     IGYNSFPLVGLTGFIMGLVLTMQLRPSLVNYGVESQLPAMVGIAIVREIGPVITALIF
                     AGKIASSMGAELGSMKVTEQIDAMEVSGTNPFKYLVVTRVAASTLMLPVLVILGDGIG
                     LAGAFIGVNIRSTTSWHLFFRQVLDNFAFGDVLPAFIKTFFFGFAVGIIGCYKGYTSS
                     KGTEGVGKAANSAVVLSSILIFIIDLVVVQVTDVLGLN"
     gene            2199..3047
                     /locus_tag="VGQ51_17935"
     CDS             2199..3047
                     /locus_tag="VGQ51_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9038524.1"
                     /translation="MTTIEQHIFPESPTTQDQPLIPEHMPTQDQPLIPEPQPTPKEQV
                     IVIEHLRKRFGSNIVLDDFNLTVGKEENVVVLGKSGVGKSVLIKCIIGLLKPDSGSIL
                     VFGQNIPGLDHEELDRVRAKVGFLFQSNALYDSMTVRKNLEFPLRRHWISISQEEVDA
                     MVMEVLDNVGLAHTVDLLPEELSGGMRKRIALARTLILKPDIILYDEPTTGLDPVTGK
                     EISGLIVEMQKKYHTASIIISHDMHCVRLTADKIAVLIDGKCYAEGSFDSLQHSADRN
                     ICQFFE"
     gene            3058..4029
                     /locus_tag="VGQ51_17940"
     CDS             3058..4029
                     /locus_tag="VGQ51_17940"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV9038525.1"
                     /translation="MEKTNIKNFKLGILVLAGLVLMISTLYVIGRNENIFGSHFVLRS
                     RFRNANGLIPGDNVRFAGLQAGTVKTVRVIDDTTIEATLLIDEEMKPFIHRNALASIG
                     TEGLIGNKVIDITPVAGRASQVQQGDRLPSQQVVSTEDMLRTLDKAGTNIVAITQGLK
                     FTVERINNSDAFWNILSDRRIALDVRSSLDNIRQASNNANAMTAQLRGLAEDITTGKG
                     SVGRLLKDTAFATALEAAVAKISSAGDQLNSAAARIGNLAGNANTLVTDSTLSANLSA
                     SMENIRKGTAAFNENMEALKHNFLFRGYFKKQEKEAQKRANQKLAGN"
     gene            complement(4033..4983)
                     /locus_tag="VGQ51_17945"
     CDS             complement(4033..4983)
                     /locus_tag="VGQ51_17945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEV9038526.1"
                     /translation="MVQIVTLTFNPCIDKSFATETIVPDRKLACTSPRVDPGGGGINV
                     ARAIGKLGGAALAIYPAGGPQGQLLTDLLREEQVPVRPVRIAGDTRENINVTESSCGR
                     QYRFVVKGPALTEKVWKECLEGLDEVLKGGYIVVSGSLPEPWPEDIFVKIRKMADDRR
                     AHLIVDTSGKGLEAALRAGVYLVKPSIRELESLALAMGLAGGEPCRTADYIVGGGLAE
                     VVVISAGPGGAMLVTKEGVREFRAPEVCKVSTVGAGDSMVAGIVLNLSRGASMEEAVR
                     YGVACGSAAVMNPGTALCRREDVDALCARVDCVVPVMLKQ"
     gene            complement(5069..6304)
                     /locus_tag="VGQ51_17950"
     CDS             complement(5069..6304)
                     /locus_tag="VGQ51_17950"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEV9038527.1"
                     /translation="MHLYEALAILTVIATVFGYINYRFIRLPDTIGVMLISVVASLSI
                     IAIGHYEPQLFREFTAFIRGIDFYTVLVRIMLGFLLFAGAIHIHANELHRLRAPILTF
                     STVSVVLSTFITGALFYWLCRIVNEPVGFIYCLLFGALISPTDPIAVLSLLKKAGIPR
                     SLEVKITAESLFNDGVAIVLFICMYEMAVSGTENLSPMNVFLLFVRQAGGGIVLGLAL
                     GYAGYLLLRSIDYYQLEIMITVSIVMGGSLLAETLGVSGPLAMVIAGIVTGNKSRLYG
                     MSDLTRDYLDKFWALVDNLLNAVLFLLIGFELLIVPFRGAWLFLGGAAIVVVLLARLL
                     SVWIPIRLLRLGSGFHRNSAGILTWAGLRGGLSLALALSLPADMHGSLFLVITYMIVL
                     FSILVQGLTIGRFAASFRR"
     gene            6447..7502
                     /locus_tag="VGQ51_17955"
     CDS             6447..7502
                     /locus_tag="VGQ51_17955"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV9038528.1"
                     /translation="MNDIPITVKRSIELLGLLAVGFILSAGKYIIVPLLLALFISILL
                     LPLFRFFRKIGIPEIAAILLPIVCLTIFAGGIGWLFYDQLSSFLQDLPAISRNVNRLL
                     DHLSIWIRKVFGYSPDEQLTILHQNNDRLLAYVESALKQTVTSLSRWFVLFGILPAYI
                     FFILFYRKIFIKFITMSVRKRGQEEAGRIIPLLERVVLRYLVGLLIQFSYIIILLGGT
                     LTLAGIKHGFLIGILFAFLNLIPYLGPLIGNILGIVIVLASSENLLDVLIVFGAIAVV
                     QFLDNNILMPRIVGAQVKINALVSIAGIFTGGALAGISGMFLAMPVIAVIKIIFDHSA
                     GFRNWGVLLGDDRSPNT"
     gene            7920..8105
                     /locus_tag="VGQ51_17960"
     CDS             7920..8105
                     /locus_tag="VGQ51_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038529.1"
                     /translation="MPSVVIYAVTTKYPKWGISKDVFLVNYFDTDGGNAIKKYKLVPE
                     NGNQRIRVEVNEHGEFS"
     gene            complement(8161..8829)
                     /locus_tag="VGQ51_17965"
     CDS             complement(8161..8829)
                     /locus_tag="VGQ51_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEV9038530.1"
                     /translation="MRTDADIQKDVMAQLKWEPFLNAAEIGVSVKDGVVTLSGRVDSY
                     NKKYGAEMAARKILGVKAVAEDIHVGVSPAYRRTDDEIAAAVVGALKYHMAVQEERIK
                     IKVEDSVVTLTGDCDWNFQRKAAEDAIRNLPGIIRVNNFIVVKTTPTYSDVNELIHKA
                     LRRQALVDADKIEIEVIGNTVYLRGTVRSVAEKDDAELAAWSAKGVTSVLNKLVVEAA
                     EYVF"
     gene            9082..9771
                     /locus_tag="VGQ51_17970"
     CDS             9082..9771
                     /locus_tag="VGQ51_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038531.1"
                     /translation="MQLVHISFLRQFREEEIALPCQDHQYRNMVLRHFSCTKKERRLV
                     DQFLERKLAHSALIGSLIDKMEKEDLNTSKQLSAYAGQMRRQFTAFAHKTPHKGRYYQ
                     SVSQALALLMETVDICREATRRRVQRLKALVAGKKAAPFHPADFRDIQLVLNQYLDNL
                     SNQHQRLTVLLEQESNGTATFRLGTRAGLSLAKLLALLQKEISAVGATAEKVEEWRRR
                     LRILIIQETYN"
     gene            complement(9835..10239)
                     /locus_tag="VGQ51_17975"
     CDS             complement(9835..10239)
                     /locus_tag="VGQ51_17975"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-independent anion transporter"
                     /protein_id="HEV9038532.1"
                     /translation="MASFKRGDNDETEGLEYSEKIDLPDGVAIFEISGPLFFGAAYKF
                     QDAIRMTESRPKVLTIRMRNAPVIDATGIRVLKEVFSQSKRRDTKLILSEISNAQIIK
                     DLADSRLLFAIGKANVTDTLPRAVERSTAILC"
     gene            complement(10211..10417)
                     /locus_tag="VGQ51_17980"
     CDS             complement(10211..10417)
                     /locus_tag="VGQ51_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038533.1"
                     /translation="MFRPKILDTLKGYGRRQLSNDLLAGLIVGLAIVAGFIISALGGS
                     RVQIGGPTGAFIVIVYGIVQTRRQ"
     gene            complement(10459..11145)
                     /locus_tag="VGQ51_17985"
     CDS             complement(10459..11145)
                     /locus_tag="VGQ51_17985"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV9038534.1"
                     /translation="MLRTETCCENPCFLCRHCLAQWKETILEERRVLHFKKGQPIIRE
                     GEKAEGLYYITAGAVKVHQSWGAGKEFILRFAGAGDVVGYRAQGAADCSPISVTTLEA
                     TTVCFITNEFVEHIYRADPSFLYTMMRLYATELRAAENRMRDLAIMPVKGRVAASLFT
                     IREVFGMDDRGYTLVPVTRLDIACHAGTTYEAVFRLLSEWVGKGFVTTEGKKIQISDD
                     AALRRLTRVD"
     gene            complement(11164..>11519)
                     /locus_tag="VGQ51_17990"
     CDS             complement(11164..>11519)
                     /locus_tag="VGQ51_17990"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEV9038535.1"
                     /translation="VQEMKAMEDSLSAAERRDVGFVLVSFDAERDDAAHLAQFAEQQG
                     LDGRWVLLHGSPEQVRELSMLLEVRYKRLEDGNYTHSNVIVILDRNGGIEKTLPGLEP
                     GKDSAVQIIGRLAAR"
BASE COUNT         2851 a         3550 c         2569 g         2549 t
ORIGIN      
        1 atcccgttcc aattctttca gccgcggcag cgtccattcc ttcaaaagcg ccgccgacgc
       61 cgggtcgaag acagtagctc ctttacaggc gatgtccagg atataccgcc ggaaaaccac
      121 gtcctcctct gtcagaaaat atcccctgac cagcggcagc tcgcccgccg cgatggaccg
      181 ataataatta gtgagctgtt tgtcattctg cgcaaaggca gtaccgatat cgctgatagc
      241 gctcataccc agaccgagca acagcccgct tttctgcgta gtataaccca taaaattgcg
      301 gtgcagcctc gcttgcctcc atgcccgata tagctcgtcc ccgggcagcg caaaatgatc
      361 cattccgata tgcgcatacc catgttccgt caataatagc tgacccatcc catacaaacc
      421 gatcttctcc cgggcagaag gcagcattac cgtatcgatc agccgctggt tgcagataac
      481 ccggggcata tgcgcatagc tataaaaggc tattctgtcg ggcgcaagac taacagatcc
      541 ttcgatcgtc cttcgcagac attccggtgt ttgtagtgga aggccataga tcagatcgaa
      601 attgacggcc gcaaaacccg cagcccgcgc ctcctccgtc gcccgccgga cattttcaaa
      661 gggctgcacg cggtttattg ccttctgcac ctccggatta aggtcctgta ccccatagct
      721 gatgcggtta aaccccaggt cagccaacgt ttccaggtgg tcccgcgtcg tattgttagg
      781 atgtccttcc acgctgaacg catgatcggg atgcactacc gccgtcgaaa gcaaaccctt
      841 taccagtcgt tcaagattgc caggcgaaaa gaaagtcggc gtacctcctc caagatgcag
      901 ctcgcggatc accggcggct gtcccattaa ttgccgataa agccgccatt cggccagcac
      961 cgcatcgata tattcctcct ccaccccgtg attggtcgtg atctttttgg tgcatccgca
     1021 ataaagacaa agagattcgc aaaaggggag gtgtatatac aggctcatcc cttcattgct
     1081 gttggactcg ttatatcgcc cgataaaatt tgccctccat tcgacacctt caatatgatc
     1141 cttccaaaaa ggaaccgtcg gatagcttgt atatcggggc gccggttggt tatatttctc
     1201 taaaaagtta gcttccattc agtggatttt cacaaatatc ccccgggacc ccgccataga
     1261 acgatgatcg cggtcaatca gcatcctgac gctcgtcaca cctgagaacg attatcccaa
     1321 acccctgacg ctcgtcacac ctgagaacga tcatcccata acatgacgag gttcaataca
     1381 aagatcatcc ccatacaata ctttagcgat acaaccgaac aacatgccgc agcccacaaa
     1441 attccggcaa ttcctcgaag aaacgggcac agtcgcccgg ttcacgggtc agtatttcgt
     1501 gcagaccggt aaacgccgtt ttgaattcag cgaactgctc cggcaatgct atcttatcgg
     1561 gtataactcc tttccgctcg ttgggctcac cggtttcatc atgggcctgg tactcacaat
     1621 gcaattgcgt ccttcgctcg tgaattacgg agtggaatcg cagctcccag cgatggtagg
     1681 cattgccatc gtccgggaga tcgggccagt cataaccgcc ctcatctttg ccgggaagat
     1741 cgccagcagc atgggcgccg aactgggctc gatgaaggtc accgaacaga tcgacgccat
     1801 ggaagtcagc ggcaccaatc cgttcaaata cctcgtggtc acgcgggtcg ccgcctctac
     1861 actcatgctg cctgtgctcg tgatcctggg cgacggcatc ggccttgccg gtgccttcat
     1921 tggggtcaac atccgtagca ccaccagctg gcacctgttc ttccgtcagg tgctggataa
     1981 tttcgcattc ggagacgtcc ttcccgcatt catcaagacc tttttcttcg gattcgcagt
     2041 cggcatcatc ggctgctata aaggctatac ttccagcaaa ggcacagaag gcgtaggaaa
     2101 ggctgcaaat tccgccgtcg tactctcctc gatactcatc tttatcatcg acctggtcgt
     2161 agtacaggtc accgacgttc tcggactcaa ttgaagacat gacaaccatc gaacaacata
     2221 tcttcccgga aagcccaacc acccaagacc aacccctcat cccggaacac atgccgaccc
     2281 aagaccaacc cctcatccct gaaccgcagc caacgccgaa agaacaggtc atcgtcatcg
     2341 aacacctccg caaacgtttc ggttccaata tcgtcctgga cgacttcaat ctgacggttg
     2401 ggaaagaaga aaatgtcgtc gtgctcggga aatccggcgt cggaaaatcc gtactaatca
     2461 aatgcatcat aggtttattg aaacccgaca gcggctccat cctggtcttc ggtcagaata
     2521 ttcccggcct ggatcatgaa gagctggacc gcgtccgggc taaagtcggc ttcctctttc
     2581 aaagcaatgc cctctatgac tccatgacgg tccgcaaaaa cctcgagttt ccgctacgcc
     2641 gccactggat cagtatctcc caggaggaag tcgacgccat ggtcatggaa gtcctcgaca
     2701 atgtcggact ggcgcatacg gtcgacctgc tgcccgagga actgtccggc ggcatgcgca
     2761 aacgcatcgc ccttgcgagg actttgatcc ttaaacccga cattatccta tatgatgaac
     2821 ccaccaccgg gctcgacccc gtcaccggca aagagatctc cgggctcatc gtggagatgc
     2881 aaaagaaata tcacacggcc tccatcatca tctcgcatga catgcattgc gtaaggttga
     2941 cggccgacaa aatagcggta ttgatagacg gaaagtgtta cgccgaaggc tcttttgaca
     3001 gtctgcagca ttccgccgat cgtaacatct gccaattctt cgaataaact ctctatcatg
     3061 gaaaaaacga atatcaaaaa tttcaaatta ggcatactgg tgctggccgg cctggtgctg
     3121 atgatatcca ccctatatgt catcggccgt aatgagaaca tcttcggctc gcatttcgtg
     3181 ctgaggtcac ggttcaggaa tgccaacggg ctgatccccg gcgacaacgt ccgttttgcg
     3241 ggtctgcagg ccggcaccgt caagaccgta cgcgtcattg acgacaccac catcgaagcc
     3301 acactgctca tcgatgaaga gatgaaaccc tttatccacc gaaatgccct tgcctccatc
     3361 ggaacggaag gcctcattgg caacaaagtg atcgatataa cgccggtcgc cgggcgtgcc
     3421 tcacaggttc agcaaggcga taggctcccg tcacagcagg tggtcagcac cgaggacatg
     3481 ctccgtaccc tcgacaaggc cggcaccaat atcgtcgcca tcacccaagg actgaagttc
     3541 accgtcgaac gtatcaataa cagcgatgcc ttctggaaca tcctctctga ccgccgtatt
     3601 gcactcgacg tccggtcctc cctcgacaat atccgccagg ccagcaacaa tgccaatgcc
     3661 atgacagcac agctccgcgg actggcggaa gacatcacca caggaaaagg gtctgtgggc
     3721 cgccttctga aagatactgc cttcgccacc gccctggagg cagcggtagc aaagatcagc
     3781 tccgccggcg atcagcttaa cagcgccgcc gcccgaatcg gcaatcttgc cggcaatgcc
     3841 aacacgctgg tcactgactc caccttatcc gcgaacctgt ccgccagcat ggagaacatc
     3901 cgtaagggaa ccgccgcctt caacgaaaac atggaagccc tcaaacacaa ctttctattc
     3961 cgcggctact tcaaaaagca ggaaaaagaa gcccaaaaac gcgccaatca aaaattagcg
     4021 ggaaattgat gattattgct tcaacataac agggaccaca cagtccaccc gcgcacacaa
     4081 cgcatccaca tcctcccgcc tgcatagcgc cgtaccagga ttcatcaccg cagcgctccc
     4141 acaagcaacc ccatatctaa ccgcctcttc catcgaagcc ccccgcgaca ggttcagcac
     4201 aatcccggcg accatgctat cccccgcgcc tactgtactt accttacaga cctccggcgc
     4261 ccggaactcc ctaacgccct cctttgtcac caacatagcg ccgccgggtc ccgccgagat
     4321 caccactact tcggccagcc caccgccgac gatatagtct gccgtccggc aaggctctcc
     4381 cccagccaac cccatggcca gcgccagcga ttccaattct cgtatactgg gcttgaccaa
     4441 atatacaccg gctcgcaaag cggcctccaa acccttaccc gaggtatcca caatgaggtg
     4501 cgcacgccta tcatccgcca tcttcctgat ctttacaaaa atatcttcag gccacggctc
     4561 gggcagactg ccgctgacta ctatataccc tcctttcaaa acctcatcca aaccctcgag
     4621 gcactccttc cacacttttt ccgtcagtgc aggaccctta accacgaatc gatattgccg
     4681 tccgcaggaa gattccgtca cattgatgtt ctccctcgta tccccggcta tccgcaccgg
     4741 cctcaccggc acctgttcct cccggagaag atccgtcaga agttgaccct gcggtccgcc
     4801 agcaggatag atcgctagcg ccgcgccccc caatttcccg attgcccgcg ccacattgat
     4861 acctccccca ccagggtcga cgcgcggcga agtacaggcg agcttccggt cggggacgat
     4921 cgtctccgtc gcaaaacttt tgtcgatgca aggattgaat gttaacgtaa caatttgtac
     4981 catgacccca acctaccgcc ttttacacaa ttacccgatg accctcatta cccggcagac
     5041 tgatcgatat cattaataaa aacccagtct acctcctgaa gcttgccgca aagcgcccaa
     5101 tggtaagacc ctgaacgagg atcgaaaaca gcacgatcat ataggtaatg acgaggaata
     5161 ggcttccgtg catgtccgcc ggcagcgaga gcgccagcgc cagcgacagt ccgccgcgaa
     5221 gaccggccca ggtgaggatg ccggcggaat tcctatggaa acctgaccca aggcgaagca
     5281 accgtatagg gatccatacc gagagaagtc gggcgagaag aactaccacg atggcggccc
     5341 ctcccagaaa gagccatgct ccgcgaaacg ggacaatcag cagttcgaag ccgatcaaca
     5401 ggaataacac tgcattaagc aaattgtcca ccaaagccca aaatttatct aaatagtccc
     5461 ttgtaaggtc gctcataccg tacaggcggc ttttgttgcc cgtgacgatc cccgctatca
     5521 ccatagcaag cgggccgctt actccgagcg tttccgcgag gagacttccg cccatcacaa
     5581 tgctcacagt gatcatgatt tccagctggt agtagtcgat agaccgcaac aagagataac
     5641 ccgcataacc aagggccagg ccaagcacga tcccgccgcc ggcctgtcgg acaaacaaca
     5701 gaaacacatt cattggcgac aagttttccg tccccgaaac ggccatttca tacatgcata
     5761 tgaaaaggac aatagccact ccgtcattga acagcgattc agccgtaatc ttaacctcca
     5821 gtgagcgggg aatacctgcc tttttgagca gactgaggac cgcaatcggg tccgttggag
     5881 agatcagcgc accgaatagc aagcaataaa taaaacctac gggctcgtta acgatgcggc
     5941 acagccaata gaacagcgcg cccgtgataa aggtggaaag taccacgctt accgtggaga
     6001 acgtaagaat cggagccctt agccgatgaa gctcgttagc gtgaatatga atggcgccgg
     6061 caaacaggag gaagccaagc atgatcctga cgagcacggt gtaaaaatcg atcccccgga
     6121 taaacgcggt aaactctcgg aatagctgcg gttcataatg gcctattgcg ataatgctca
     6181 gggacgcaac cacagagatc agcatcacgc cgatcgtatc gggcagccgg ataaaccggt
     6241 agttgatata cccaaaaacg gttgcgatga ccgtaagtat agccagcgcc tcatacaggt
     6301 gcataagcta aaggtaagcc aatgggaaca atatgccatt gctgccactg aagatcatcg
     6361 tttaccctga ccagcgtcac cctttgcagc ggcaacttct tctaaattac cggaatgaaa
     6421 tcgattttaa tcgccaccgc ctccctatga atgatatacc tatcacagtc aagcgctcca
     6481 tcgagctgct cggcctcctt gccgtaggat tcatcctctc ggcagggaag tatatcatcg
     6541 taccccttct gctcgcgctt tttatcagca ttcttctcct tccgctgttc cggttcttcc
     6601 ggaagatcgg aattccggaa attgctgcca tcctgcttcc gatcgtctgc ctgacaatct
     6661 tcgccggcgg tatcggctgg cttttctatg accagctctc ctcgtttctt caagacctcc
     6721 cggccatctc gcgcaatgtc aacaggctcc tcgatcatct aagcatttgg atccgaaagg
     6781 tctttggtta ttctcccgac gagcaactga cgattcttca ccaaaacaac gacagacttc
     6841 tcgcttacgt cgaaagcgca ctcaaacaaa cggtgacgtc actttccaga tggtttgtac
     6901 tattcggcat ccttcccgcc tatattttct tcatcctctt ctatagaaag attttcataa
     6961 agtttataac catgtctgtc aggaaaagag gacaggaaga agcaggccgc atcattccat
     7021 tacttgaacg ggtggtcttg cggtatcttg tcggcctgct catccaattc agctatatta
     7081 ttatcttgtt aggcggcaca ttgaccctgg cggggatcaa acacggcttc ctgatcggta
     7141 tcctcttcgc attcctgaac ctcatcccgt acctgggacc cctgatcgga aatatcctgg
     7201 ggattgtcat cgtgcttgcc tcttcggaaa acctattgga cgtgcttatc gtcttcggtg
     7261 ccatcgccgt tgtccagttc ctcgacaaca acattctgat gccccgtatc gtgggcgcac
     7321 aggtaaagat caacgcgctc gtttcgattg cagggatctt caccggcggg gccctggccg
     7381 gtatttcagg aatgtttctt gcaatgcccg taatagccgt cataaaaatt attttcgatc
     7441 attccgccgg cttcaggaat tggggcgtat tgctgggtga cgaccgatcc ccaaatacct
     7501 gaagacatga tcatttccgg ggggtcggat ttccggagca ataggctctt tcggctcctc
     7561 ttccggcctg atctcgggca tccgctcagg ataaaccggc tcctcgttcg gggtttcagg
     7621 aactacctcc ggcgtcttgt ttggcatcgg gatctccgaa ggcttttccg ccggcttccg
     7681 ctttttcacg tcataagctt cggtcatatt ttcttttcgc taaaaatacg cattacaaac
     7741 ccgaccatac ttgaccacga tcaacgtctc ccgtgacccc tatcagcttt actgcccctt
     7801 ctatgaccta gctttaagcg ataatcctac actgctccgg cgatagctca gcagcccctg
     7861 cccagggtca cgcaaatgga aaattgctgc gtaccgcaga aaaatacaaa aacgctcttt
     7921 tgccgtctgt cgtcatttat gcagtgacca ccaaatatcc caaatggggc attagcaaag
     7981 atgtgttcct cgtaaactat ttcgacaccg acggtggaaa tgcgatcaag aagtacaaac
     8041 tcgtcccgga gaacggtaac caacgcatca gagtcgaagt gaatgaacac ggcgaatttt
     8101 cctgacaata agatcgcccg ccaaagcggg cgatctctca tacctccctt ctaataatcc
     8161 ctaaaaaaca tactccgccg cttcgaccac cagcttattc agcacgctcg taacgccctt
     8221 tgccgaccag gcggccaact ccgcatcgtc cttttccgca accgaccgta ccgtccccct
     8281 cagatagacc gtattgccta tcacctcgat ctcgatctta tcggcatcta ccaaagcctg
     8341 acgtcttagc gccttgtgga tcagttcgtt gacatcggaa taggtcggag tcgtctttac
     8401 aacgataaaa ttattgaccc gaatgatccc cggcagattt cggatggcat cttcagcagc
     8461 cttacgctga aaattccagt cgcagtctcc tgtcaatgta acaacgctgt cttcgacctt
     8521 gatcttgatc ctttcctcct gaacggccat atggtatttt aaggcaccca cgaccgcggc
     8581 ggcaatctcg tcgtccgtcc tccggtacgc cggcgataca cccacatgga tatcttcggc
     8641 cacggccttc actccaagga tctttctcgc cgccatctcg gccccgtact tcttgttata
     8701 actgtccacc cgccctgata acgttacgac accgtctttt accgatacgc cgatctcggc
     8761 agcattaaga aatggctccc acttcaactg tgccatcacg tctttttgaa tgtctgcatc
     8821 tgttctcata taaatatgtt ttatagtgaa aaatctgttc actcataaag ataccacagg
     8881 ccaaaacccc ggaaaagaac cccggtcagc agtggagatg agactggtta ccaacatgtg
     8941 ccaaattgat cccgttcagc gagacttgtc acacctgagt ttcatcagtg caaccacagg
     9001 gagaagatca ttccgatacc tgatcctgat cctgatttac cccctgtccc gatccttacc
     9061 tttccttaaa atctttcagc catgcaactc gtgcacatat cattcctccg gcaattccgt
     9121 gaagaagaga tcgcacttcc ctgccaggat catcaataca ggaacatggt gctcagacat
     9181 ttctcctgca ccaaaaaaga acgccgtctt gtcgaccagt tcctcgaacg gaagcttgcc
     9241 cattcggcac tcatcggctc gctgatcgat aaaatggaaa aggaagacct gaataccagc
     9301 aagcaactgt ccgcgtacgc ggggcaaatg cgccggcaat tcaccgcatt tgcccataag
     9361 acaccacaca aaggacgcta ttaccaaagt gtctcgcaag cgcttgccct cctcatggaa
     9421 accgtggaca tctgccggga agcgacccgc cgccgtgtac agcgcttaaa agccctggtt
     9481 gccggaaaaa aagcagcccc ttttcatccc gcagactttc gcgatattca actggtactg
     9541 aatcaatacc tggataacct ctccaatcag caccagcggt tgaccgtcct gctggaacag
     9601 gaatccaacg ggaccgcaac gttccgtctc ggcacccgcg ccggactctc cctggcaaaa
     9661 ctgctcgccc tgctgcaaaa agagatcagt gcagtcgggg ccaccgccga aaaagtcgaa
     9721 gaatggcgca gaagactccg tatcctcatc atccaggaaa catacaattg aacacaacac
     9781 tctcgctccg gcgcacgcgt gaatcccaca gcgcagctaa accaaaagca tcgctcagca
     9841 caaaatagcc gtactccgtt cgaccgcgcg tggcaatgta tccgtcacat tggccttccc
     9901 aatggcgaag agcagccggg aatcagcgag gtccttgatt atttgagcgt tggaaatttc
     9961 cgagaggatc agtttcgtat cccgccgttt cgattggctg aatacctctt ttagcacccg
    10021 gatacctgta gcatcgatca ccggtgcatt gcgcatccgg atggtcagca cctttggtcg
    10081 gctttcggtc attcggatgg catcctgaaa cttatacgca gcgccaaaaa ataaggggcc
    10141 ggatatttca aaaatggcca ccccgtccgg cagatcgatc ttttcggagt actccaaccc
    10201 ttcggtctca tcattgtctc cgcgtttgaa cgatgccata aacgataacg atgaaagcgc
    10261 cggtgggacc tcctatctgg accctgctcc cgccaagcgc cgaaatgatg aatcctgcca
    10321 cgatcgccag ccccacgatc aggcctgcca gcaggtcatt actgagttgc cgccttccgt
    10381 atcctttcag ggtgtcgagt attttgggtc tgaacatgcg ggtaaatgag tagtgaataa
    10441 ttacttgaaa gtcgcgcatc aatccacccg agtcaaacgc cgcaatgctg cgtcgtcact
    10501 gatctgtatt ttctttccct ccgtagtgac aaatcccttc cccacccact cgctcagaag
    10561 ccggaatacc gcctcatacg tcgttccggc atgacaggca atatccagcc gtgtgaccgg
    10621 caccagcgta tatccccggt cgtccatccc gaatacctcc cggatcgtaa acagcgacgc
    10681 agccaccctc cccttcaccg gcataatcgc cagatcgcgc atcctgtttt ccgccgcgcg
    10741 cagttccgtc gcatacagcc gcatcatggt atacagaaaa gacggatcag cgcgataaat
    10801 atgttcaacg aactcattgg taataaagca taccgtcgtg gcttccaggg ttgtcactga
    10861 aatgggagaa cagtccgctg ccccctgcgc ccggtaaccg accacatcgc cggccccggc
    10921 aaaacgcaaa atgaactcct tcccggcgcc ccatgactga tgcaccttta ccgcgccggc
    10981 cgtaatataa tatagccctt cggccttttc accctccctg atgataggtt gccctttctt
    11041 aaaatgcaac acgcgtcttt cctccaaaat tgtctctttc cactgcgcaa gacaatgccg
    11101 gcagaggaaa cagggatttt cgcaacaagt ttccgttctt agcatagctc aacatttaga
    11161 tgatcagcgg gcagccagcc gaccgatgat ttgaacagcc gaatccttcc ccggctccag
    11221 acccggcagc gtcttttcga taccaccgtt cctgtccagt atgacgatga cattcgaatg
    11281 ggtataatta ccgtcctcca ggcgcttata tcgaacctcc aatagcatcg ataactccct
    11341 cacctgctcc ggactaccat gtagcaatac ccaacgaccg tccaaacctt gctgttcggc
    11401 gaactgcgcc agatgcgcag catcatccct ttccgcatcg aatgatacca gcacaaatcc
    11461 tacgtccctt ctttcagcgg ccgaaagtga atcttccatg gccttcattt cctgcacta
//
LOCUS       DASYSV010000577         6666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_499750, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000577 DASYSV010000000
VERSION     DASYSV010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499750"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VGQ51_17995"
     CDS             <1..1096
                     /locus_tag="VGQ51_17995"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038536.1"
                     /translation="AWDFRRSFLQSRDCAARFASFLPASMLSGAMEVVAGTIPDGYKW
                     LSNLLGIKGSIAGGMKKLDRFLAADDVYASLYHDEAVFYYLYLQFYLSNKRAEVFAFI
                     GQHQLDVCNNHLFTYLYANLCINDQRSAPALMALRQMNPSPEYLDMPVWDQEMGYACL
                     NHLDPEAVEYFQRWLARFKGKFYVKDVLQKMSWYYYLKGDMVRADSCRRQVLVRGTAT
                     SDADRQALKEAKSGRWPNRVLLKARLLDDGGYFSEALQVLQGMEPSELRDQVERCEYT
                     YRLGRIYDGLGKDEEAVAAYAAAIRLGEHLKEYYAARAALQAGYIYEQRGDKAKAISL
                     YEKCLSMPEHDYKNSLDQRAKAGVARCKGE"
     gene            1158..2846
                     /gene="recN"
                     /locus_tag="VGQ51_18000"
     CDS             1158..2846
                     /gene="recN"
                     /locus_tag="VGQ51_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV9038537.1"
                     /translation="MRTSGDGARSDMLQRIQIQNYAIIDELSIDFSSQLNVITGETGA
                     GKSILMGALSLILGDRADTMVLLRRDKKCVVEGVFDVAGRMEVLAFLRTCDLDADEEV
                     VIRREIAPSGKSRAFVNDTPVNLDQLRQLSSLLVDLHRQFDTLELGENRFQLEVMDAL
                     AGQGEALASYRAVYREWQTAARELATLRGQQQQFNKERDYNQFLFDELNEASFLENEL
                     EELDQELKLLSNSEGIKTALSKIYYKMQEGEQPLVQQLRVLQHTMEGYAVYDAELARL
                     SERMSSVQIELQDIAEEVDRVNERVTYDPRRIEVINERLSIGYRLMKKHGVPTTAALL
                     DLARQLEHKLKDSLQVDAEIAAGEAAVQRLLEEARTKAAVLTKGRLSQVGPLEGQVNK
                     LLKQVGMPNARLKVQVEAEAPGIDGADAVEFLFDANRSNRFESIRKVASGGELSRLML
                     CIKSLVAESIDLPTLIFDEIDTGISGEAARQVGIILKNLARRRQVICITHQPQIAGKA
                     DAHYFVYKAAKGESVQTNIRLLSQDERITTIAQMLSGEKPTAAALENAREMVLS"
     gene            2843..3049
                     /locus_tag="VGQ51_18005"
     CDS             2843..3049
                     /locus_tag="VGQ51_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038538.1"
                     /translation="MRGRSLLILLLLVLALVPMYYVNRWLQLVMRPRENAGRFFLFLF
                     ANFVLIIVYTVFLVGLVVRIFPVR"
     gene            3084..3896
                     /locus_tag="VGQ51_18010"
     CDS             3084..3896
                     /locus_tag="VGQ51_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV9038539.1"
                     /translation="MSYNLLKGKKGIVFGALDERSIAWRTALRCHEEGAQLVLTNAPV
                     ALRMGEINSLAEAVKAPVIGADVTNMDDLKKLFEESMKHFGGGIDFILHSIGMSLNMR
                     KGKSYTEIDYEWNKKTLDISAMSLHRVLRTAWDMDALNEGASVVALTYIAAQRVFPDY
                     NEMADAKALLESVTRSFGCYYGVKKKVRVNTVSQSPTRTTAGSGVKGFDGFVTYAEKM
                     SPLGNASADQCAGYCVTLFSDLTKMVTMQNLFHDGGFSFTGVTEAVIRDMKE"
     gene            complement(3941..4162)
                     /locus_tag="VGQ51_18015"
     CDS             complement(3941..4162)
                     /locus_tag="VGQ51_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEV9038540.1"
                     /translation="MFWTLELASYLEDAPWPATKDELIDYAIRSGAPIEVVENLQELE
                     DEGEIYEGIDDIWPDYPSQEDFFFNEDEY"
     gene            4311..4967
                     /locus_tag="VGQ51_18020"
     CDS             4311..4967
                     /locus_tag="VGQ51_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9038541.1"
                     /translation="MLTAKGIQKYYGSLWVLKGVDMQIAKGEIVSIVGPSGSGKSTLL
                     HILGTLDEPAEGEIFLQSKRIHDLKGRPLAAFRNKHIGFVFQFHHLLPEFSALENVCI
                     PGWIAGRRKKEVEEQALGLLQVLGVGHRTENKPGALSGGEQQRVAVARALINKPDIVF
                     ADEPTGNLDTANAKELHQLFFDLRDRFSQTFLIVTHNEELAQMSDRVVHMKDGLLVNH
                     "
     gene            complement(5098..5715)
                     /locus_tag="VGQ51_18025"
     CDS             complement(5098..5715)
                     /locus_tag="VGQ51_18025"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEV9038542.1"
                     /translation="MASKIYTKTGDKGTTSLFGGTKVPKSHLRIEAYGTVDELNSFMG
                     LLSDHLASAASNAAILASISHSENPAFDFDETGGQLHEIQDRLLTIGSLLATEKETKY
                     KLPDLLPDDIQNLENAIDDMDAVLPHLKSFVLPGGHIAVSTAHITRCVCRRAERCCVR
                     LQEEGAALDPLIIQYLNRLSDYLFTIARFAAHRLQIHEIPWTPRK"
     gene            complement(5767..6477)
                     /locus_tag="VGQ51_18030"
     CDS             complement(5767..6477)
                     /locus_tag="VGQ51_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9038543.1"
                     /translation="MTSTPIIHLDSIRKSYLMGKQALQVLKGVSLDISKNEYVALMGP
                     SGSGKSTLMNILGCLDTPTSGTYVLNGQDVSKMHDDDLADVRNKEIGFVFQQFNLMPR
                     YTALENVAFPLVYSGISRKQRLERAMAVLEKVSLADRSHHKPNELSGGQCQRVAIARA
                     LINNPSLILADEPTGNLDTKTSVEIMDIFTSIQAAGNTVVLVTHEEDIAKYAHRIVRL
                     RDGVIESDRRHIKEVLTT"
     gene            complement(6474..>6666)
                     /gene="gatC"
                     /locus_tag="VGQ51_18035"
     CDS             complement(6474..>6666)
                     /gene="gatC"
                     /locus_tag="VGQ51_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV9038544.1"
                     /translation="IQFVEKLGELDTTGVEPLLHMSPETNVLRDDIPQASISRQEALS
                     QAPATDGIFFEVPKVIRKQ"
BASE COUNT         1440 a         1371 c         2280 g         1575 t
ORIGIN      
        1 cgcgtgggat ttccggcgca gtttcctgca gagcagggat tgcgcggcgc ggttcgcatc
       61 ctttttaccg gcgtcgatgt tgagcggggc gatggaagtg gtcgcgggca cgatccccga
      121 cgggtataaa tggctgagca acctgctggg tatcaaggga agcattgccg gcgggatgaa
      181 aaagctggat cgttttctcg cggccgatga cgtctatgca tcgttgtacc acgatgaagc
      241 ggtcttctac tatctttatc tgcaattcta tctttcgaac aagcgggcgg aagtgttcgc
      301 ttttatcggg cagcatcagc tggatgtgtg caataatcac cttttcactt atctctatgc
      361 caatctatgt atcaatgatc agcgatcggc gcctgccctg atggcgctcc ggcagatgaa
      421 cccgtcgccg gaatacctgg acatgccggt gtgggaccag gagatgggtt atgcctgtct
      481 gaatcacctg gatccggagg cggtggaata ttttcaacgt tggctggcgc gattcaaggg
      541 gaaattctat gtgaaggacg tattgcagaa aatgagctgg tattattatc tgaaaggcga
      601 tatggtcagg gccgacagtt gccggcggca ggtgttggtg cgcgggacgg ctacatcgga
      661 tgcggacaga caggcgttga aggaggcaaa gagcggtagg tggcccaaca gggtgttgtt
      721 aaaggcgcgg ttgctggatg acggaggtta tttttcggag gcgctgcagg tgctgcaggg
      781 aatggaacct tcggaattgc gggatcaggt ggagcgttgc gaatatactt accggctggg
      841 gcgtatctat gatgggttgg gcaaggatga agaggcggtg gccgcttacg cggccgcgat
      901 ccggttgggg gagcatttga aggaatatta tgcggcgcgt gcggcgttgc aggcggggta
      961 tatttatgag cagagggggg ataaggcgaa ggcgatctcg ctttatgaga agtgtttgtc
     1021 gatgcctgag catgattata agaactcgct ggatcagcgg gcgaaggcgg gggttgcgag
     1081 gtgcaagggt gaataggcgg gtgagttggc ggtgtgggaa ttttttaaga agtggatggg
     1141 gtgaaatcct taatttggtg cggacaagcg gggatggagc ccgcagcgat atgctacaac
     1201 ggatccaaat acaaaactat gcgatcatcg atgaattgag catcgatttt tccagtcaat
     1261 tgaatgtgat cacgggggag acgggggcgg gtaaatcgat cctgatgggg gctctttcgc
     1321 tgattttggg cgacagggcc gatacgatgg tgctgttgcg gcgggacaaa aaatgcgtgg
     1381 tggaaggcgt attcgatgtg gccgggcgga tggaagtttt ggcatttttg cggacctgcg
     1441 acctggatgc cgatgaggag gtggtcatcc ggcgggagat agcaccatcg ggtaaatcgc
     1501 gggcgttcgt caacgatact ccggttaatt tagatcaact gcgacaactg tcgtccctgc
     1561 tggtggatct tcaccggcag ttcgatacat tggaactggg cgagaaccgg tttcaactgg
     1621 aagtgatgga tgcgcttgcg gggcaggggg aggcgttggc ttcctaccgg gcagtctacc
     1681 gggaatggca gaccgcggcg cgcgagctgg cgaccttgcg cgggcagcag cagcaattca
     1741 acaaagagcg ggattataat caattccttt tcgacgagct gaatgaggca tcgttcctgg
     1801 agaatgagtt ggaggaattg gatcaggagc tgaagctgct gagcaattcg gaaggcatca
     1861 agacggcctt gtcgaagatc tattacaaaa tgcaggaggg ggagcagccg ttggtgcagc
     1921 agttgcgggt attacagcac acgatggagg ggtatgccgt ttatgatgcg gagctggcca
     1981 ggttgtcgga gcgtatgtct tctgtgcaga ttgaactaca ggatatcgcg gaggaggtcg
     2041 atcgggtgaa tgagcgtgtg acctatgacc cgcggcggat cgaggtcata aacgagcggt
     2101 tatcgatagg ataccggctc atgaagaagc atggcgtgcc gacgaccgcg gcgttgctcg
     2161 atctcgcgcg gcagctggag cataaattaa aggattcgct gcaggtggat gcagagatag
     2221 cggccggaga ggcagcggtc cagcgattgt tggaggaggc aaggacgaag gcggccgtgc
     2281 tgacgaaggg gcgtttgtcg caggtggggc cgctggaagg gcaggtaaac aagctgttga
     2341 agcaggtggg catgccgaat gcgcgattga aggttcaggt ggaggctgag gcgccgggga
     2401 tcgatggcgc ggatgccgtg gaatttcttt tcgatgccaa tcgaagcaac cggttcgagt
     2461 cgatccgcaa agtggcatcg ggtggggaac tgagccggct gatgttgtgc atcaaatcgc
     2521 tggttgcaga atcgatcgat ctgcctacgc tgatcttcga tgagatagat acggggatat
     2581 ccggggaggc tgccaggcag gtgggcatca tccttaagaa cctggcgaga cggcgacagg
     2641 tcatctgtat cacgcatcag ccgcagatcg ccggcaaggc ggatgcacat tattttgtct
     2701 acaaggctgc aaaaggggaa tccgttcaaa ccaatatacg gctgctgagc caggatgagc
     2761 gtatcaccac tattgcccag atgctgagcg gggagaagcc gaccgcagcc gcgctggaga
     2821 atgcccggga gatggtatta tcatgagagg gagatcgttg ctgatattgt tgttattggt
     2881 gctggcactc gtgccgatgt actatgttaa ccggtggttg cagctggtga tgcggccgcg
     2941 ggaaaatgcg ggacgttttt ttcttttcct gtttgcgaat tttgtgttga tcatcgtcta
     3001 tacggtgttc ctcgtcgggt tggtcgtgcg gatttttccg gtgaggtgag gggggaaggg
     3061 ttatatttgc gaaaattcaa tctatgtcat acaacctgct taaaggtaaa aaggggatcg
     3121 tattcggagc gttggatgag cggtccatcg cctggcggac ggcgttacgt tgtcatgagg
     3181 aaggggcgca gctggtgctg accaatgcgc cggtggcttt gcggatgggc gagatcaaca
     3241 gtcttgcgga agcggtgaaa gcgccggtca tcggtgcgga tgtgacgaac atggacgatc
     3301 tgaagaagct gttcgaggag tcgatgaagc atttcggcgg agggatcgat tttatcctgc
     3361 attccatcgg gatgagtctc aacatgcgga agggaaagag ctacaccgaa atagattacg
     3421 agtggaacaa gaagacgctt gacatttcgg cgatgtcgct gcatcgcgtg ctcaggacgg
     3481 cctgggatat ggatgccctg aatgagggcg ccagcgtggt ggcattgacg tatatcgcgg
     3541 cccagcgtgt ttttccggat tacaatgaga tggcggatgc caaggcgttg ctggaaagtg
     3601 tcacgcggag ttttgggtgc tattatggcg tgaagaagaa agtgcgcgtc aatacggtat
     3661 ctcagtcgcc gacgaggact actgcgggca gcggggtgaa gggattcgac gggtttgtca
     3721 cttatgcgga gaagatgagc ccgctgggta atgcgtcggc ggatcagtgt gccggttatt
     3781 gtgtgacgtt attcagtgat ctgacgaaga tggtgactat gcaaaatcta ttccatgacg
     3841 gcggattctc gtttaccgga gttaccgagg cggtgatacg cgatatgaaa gaataagatg
     3901 taaaagagaa gcgcccgtca gggcgctttt tttatgagtt ttagtattcg tcttcgttga
     3961 aaaagaaatc ttcctggctg ggatagtccg gccagatgtc atcaattcct tcatagatct
     4021 ctccttcgtc ttccagctcc tgtaaattct ctactacttc gatgggagca ccggaacgga
     4081 tggcgtagtc gatcagttca tcttttgtag ccggccaggg ggcgtcttcg agatagctgg
     4141 ctaattcgag tgtccaaaac atcgttctta atttttcgca aaagtaattt ttaaaatgat
     4201 tttaccaaat ttctctatca aaaaccggtc tagcgggtgt gggagaaggc cgattgtcga
     4261 atgtaaactg taagtttgcg ctaacggaac gcggggaata acttaatagt atgttaacag
     4321 ccaaagggat acaaaaatat tatggaagcc tgtgggtact gaagggagtc gacatgcaaa
     4381 tcgcaaaagg ggagatcgtg agtatcgtgg ggccttccgg ttccgggaaa agcacgctgc
     4441 tgcatatcct gggcacgctg gacgagccgg ccgaaggcga gatcttcctg caaagcaaac
     4501 gcatccatga tctgaagggg cgcccattgg cggccttccg gaacaagcat atcggatttg
     4561 tttttcaatt ccatcatctt ttgccggaat tcagtgcgct ggaaaatgtt tgtataccgg
     4621 gatggatagc ggggcgacgg aagaaagaag tggaagagca ggcgttgggg ctgctccagg
     4681 tgctgggtgt cggtcatcgc accgaaaata agccgggcgc cctatcgggt ggggagcagc
     4741 aaagggtagc ggttgcgagg gcgctgatca ataaaccgga tatcgtgttc gcagatgagc
     4801 cgacggggaa cctggatacg gcgaatgcga aggagttgca tcagttgttt ttcgatctcc
     4861 gggacaggtt ttcacagact tttcttatcg tgacccataa tgaggaactg gcgcaaatga
     4921 gcgacagggt ggtgcatatg aaggatgggt tgcttgtaaa tcactagaaa ttaatggatt
     4981 gggctgcgct ggtgaggtgc gggtcgggtg ttggcggccg ctcggggcgg gtggaggctg
     5041 acggctgtct caggcggtgc gggtcggtcg ctgacgggcg cacggggcgg gtggaggcta
     5101 tttgcgggga gtccagggaa tttcgtggat ctggagccgg tgagcggcga agcgggctat
     5161 ggtgaaaaga tagtcgctga ggcgattgag gtattgtatg atgaggggat cgagggcggc
     5221 gccttcttcc tggaggcgga cgcagcatct ttcggcgcgg cggcagacgc agcgggtgat
     5281 gtgggcggtg gaaacggcga tatggccgcc gggaaggaca aaagatttga gatgcgggag
     5341 gacggcatcc atatcgtcga tggcgttttc gagattttgg atgtcgtcgg gaaggaggtc
     5401 cggtagtttg tatttggttt ctttttcggt ggcgaggaga gagccgatgg tgaggaggcg
     5461 gtcctggatc tcgtggagct ggccgcctgt ctcgtcgaaa tcgaaagccg gattctctga
     5521 atgggaaatt gaagcgagga tggctgcatt ggatgcggcg gatgccagat ggtcggagag
     5581 cagacccata aaagaattga gctcgtcgac ggtgccgtaa gcttcgatac ggagatggct
     5641 tttggggact ttcgtgcctc cgaagaggga ggtagttccc ttgtcaccgg tttttgtata
     5701 gatttttgag gccatggttg ggggagagat tagatttttg aggccattgg ggggacgcgg
     5761 tttcggttag gtggtgagga cttctttgat gtggcggcgg tcgctctcta tcacgccgtc
     5821 gcgcagccgc acgatccggt gggcatattt ggcgatgtct tcttcgtgtg taacgaggac
     5881 tacggtgttg ccggcagcct ggatgctggt aaagatgtcc atgatctcga cggatgtctt
     5941 ggtatcgagg tttccggtgg gctcgtccgc gaggatcagc gaggggttat tgatcagggc
     6001 cctggcgatg gcgacgcgct ggcattggcc tcccgacaac tcgttgggtt tgtgatggct
     6061 gcggtcggcg agggagactt tttcgaggac ggccatggcc ctctcgagtc tttgtttgcg
     6121 cgagatgccg gaatagacga gcgggaacgc gacgttttcg agcgcggtat agcgaggcat
     6181 caggttaaac tgctggaaga caaacccgat ctctttgttc cggacgtcgg ccagatcgtc
     6241 gtcgtgcatt ttgctgacat cctgaccatt gaggacatag gtgccggagg tcggggtgtc
     6301 aagacagccc aggatgttca tgagagtgct ctttcccgaa ccggacggcc ccatgagggc
     6361 aacgtattca ttcttcgaga tatcgaggga aacgcctttc aggacctgta gggcctgttt
     6421 gcccatgaga taggatttgc gtatgctgtc gaggtgtatg atcggggttg atgtcattgt
     6481 tttctgatta ctttcgggac ttcgaaaaag atgccgtcgg tggccggggc ctggctgagg
     6541 gcctcctggc ggctgattga agcttggggt atgtcatccc tcaggacgtt ggtttcgggg
     6601 ctcatgtgga gcaatggttc gacgccggta gtatcgagct cgcccagttt ttcgacaaat
     6661 tggatc
//
LOCUS       DASYSV010000578         3976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1270408, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000578 DASYSV010000000
VERSION     DASYSV010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270408"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VGQ51_18040"
     CDS             <1..186
                     /locus_tag="VGQ51_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA-phosphatase"
                     /protein_id="HEV9038545.1"
                     /translation="AAVLGHILPARAGAYMSMAQEAANSRVYAGIHYTVDCTAGFTVG
                     TNVGNYAVKRAQGDGAE"
     gene            273..1859
                     /gene="treF"
                     /locus_tag="VGQ51_18045"
     CDS             273..1859
                     /gene="treF"
                     /locus_tag="VGQ51_18045"
                     /inference="COORDINATES: protein motif:HMM:NF009774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HEV9038546.1"
                     /translation="MKRIPGLLFIFLTLAHLSFGQPIVTTPPDKVYGKLFVDVQMERV
                     FPDGKTFVDCIPKRKPADIVADYETLRTVAGFDLKKFVFDNFDLPASPTNTYKTNTSE
                     DVVTHIRNLWPVLRRNPDKVVEGSSLLPLPYPYIVPGGRFREVYYWDSYFTMLGLKES
                     GEIGMIDDMVKNFAYLIGNYGHIPNGNRTYYLGRSQPPFFSVMVELLAEAKGDTVYQE
                     FLPAMEKEYAFWMDGADRLKPGQAYRRVVRLDNGDILNRYWDDATVPRQESWREDVLT
                     AQRSGRDKVEMYEELRAAAESGIDFSSRWFADGKTLVTIRTSDMVPPDLNALLYHLEW
                     AIAKAKLMNKDSSAEVFRKKAVHRGELIDRYCWNKTLSFYTDYNFKTRKQSSSINPAG
                     MYPFCFINEHPDYMSLLGKRAANVIRAKLLRPGGVATTGFDTGQQWDLPNGWAPLEWM
                     TIWGLDRCGQKELAADIAARWVKLVEKVYKQTGKLMEKYNVADIDKEAGGGEYPGQDG
                     FGWTNGVLLKLIDMYHLPKQ"
     gene            1969..2808
                     /locus_tag="VGQ51_18050"
     CDS             1969..2808
                     /locus_tag="VGQ51_18050"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV9038547.1"
                     /translation="MKTSLYLLAAATFLFSACSSNNVTVDDSLGHYFDSAGVKGTFAL
                     LDNGQGRFTIYNLPRYRDSLYPAAQTFDILQTLVAFQTGILPDEKKTAIVEDSSTADP
                     LSPGNPIDSKTTVTYAWAMRDPGPVGDRVYRQLADSIGIDTLKKWVDSLQYGDKDIKA
                     DSGRVLIRGIRINADQQLGLVKKLYFNQLPFFRRPQDLVKGMLTTEVNSNYKLVYKTG
                     RAAYNPGPAIGWVIGWVQENEHPYFFVINLESPDGHSDVETIGIQLAKKILPKLGFFQ
                     GKK"
     gene            2871..>3976
                     /locus_tag="VGQ51_18055"
     CDS             2871..>3976
                     /locus_tag="VGQ51_18055"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV9038548.1"
                     /translation="MFRIEHIEYLVGLAAIPLLLATLYFLLSWKKKTAGRIGDPVLVR
                     QLIRNFSSWRFLLKAALALLAFIVIVLGAGNPQKPGSMQNIQRKGVDVMFVLDVSKSM
                     LARDIKPSRLEKAKQLMLQLVDKLDNDRIGLVLFAGRPYMQMPLTTDHGASKMYIQQA
                     SPDAVPTQGTVIGEALQMANGAFDARDTKYKSIILISDGEDHDPDARKIAKKLAQDGV
                     MINTVGIGTPDGSPIVDPATGELKKDDQGNVVMSKLNEAELQQLATSTNGQYIRLDNV
                     DDALITMSQQLDNAGKKSVTDAELIDYKSFFQWFLAVALVLLVVEYLVSERRKKVPAV
                     VPALFLLALGCATLPASAQSTNARIRSGNRYYKK"
BASE COUNT          981 a         1108 c         1026 g          861 t
ORIGIN      
        1 gccgccgtac tgggtcatat ccttccggca agggcgggcg catatatgtc gatggcgcag
       61 gaagccgcca actcccgcgt atatgcgggt atccattata ccgtcgactg tacggcagga
      121 ttcacggtcg gtactaatgt aggcaactat gccgtaaaac gcgcccaggg cgatggcgcc
      181 gaataaggag caattagttt tgattatcat aagcaaaagc aagcaaacac cgcgattctt
      241 cgcggtgttt cattttctac ttatttttag gtatgaaacg aatcccaggc ctcttattca
      301 tatttctaac cctcgcgcac ctttccttcg gacagccgat agtcactacc ccgcccgata
      361 aagtctatgg gaaattattc gtcgacgtcc agatggaacg cgtattcccc gatggcaaga
      421 ccttcgtcga ctgcatcccc aaacgcaaac cggccgatat cgtggccgat tatgaaacgc
      481 tgcggacggt ggcgggtttc gatctgaaaa aattcgtgtt cgacaatttc gatctgccgg
      541 caagccctac caatacctac aagaccaata cttcggaaga cgtagtcacg cacatccgca
      601 atctctggcc ggtgctgaga aggaatcccg ataaggtcgt cgaaggcagc tcgctgctcc
      661 ctttgcctta tccttatatc gtccccggcg gccgcttccg tgaagtgtat tactgggact
      721 cctatttcac gatgctcgga ctgaaggaga gcggcgagat cggcatgatc gacgatatgg
      781 tgaaaaattt cgcctacctc atcgggaact acggacatat tcccaacggc aaccggacct
      841 attatctcgg tcgttcgcag cctccatttt tttcggtgat ggtcgaattg ctggcagagg
      901 cgaaggggga taccgtttac caggaattcc tgccggcgat ggagaaggaa tatgccttct
      961 ggatggacgg cgcggaccgg ctgaagcccg gacaggctta tcggcgggtg gtcaggctgg
     1021 ataacggcga tatcctcaac aggtattggg atgacgccac cgtcccccgc caggaaagct
     1081 ggcgcgaaga cgtgctcacc gcgcagcggt caggtcgtga caaggtggaa atgtatgagg
     1141 agctgcgtgc cgctgcggaa agtggcatcg acttcagcag ccgctggttt gccgacggga
     1201 agaccctggt cactatccgc accagcgata tggtcccccc cgatctgaat gccctgctct
     1261 atcacctcga gtgggccatt gccaaggcca aacttatgaa taaggacagc agcgcggaag
     1321 tctttcggaa aaaggccgtt catcgcggcg agctgatcga caggtattgc tggaataaga
     1381 cactcagttt ttataccgac tacaatttca agacccggaa acaaagcagc agcatcaatc
     1441 cggcaggcat gtacccgttt tgttttatca atgaacatcc cgactatatg agcctgctgg
     1501 gaaaaagggc tgcaaatgtc atccgcgcca aacttctcag acccggtggc gtagccacta
     1561 ccggattcga tacgggtcag caatgggatc tgcccaatgg ctgggccccc ctcgaatgga
     1621 tgaccatctg gggactggat cgctgcggac aaaaagagct ggccgccgat atcgctgcac
     1681 gctgggtaaa actcgtcgaa aaggtctata aacagaccgg caagctgatg gaaaaataca
     1741 atgtcgcgga tatcgacaaa gaagccggcg gtggagaata tcccggccag gacggtttcg
     1801 gctggaccaa cggggtgttg ctgaaactga tcgatatgta tcatttgccc aagcagtaag
     1861 acggcccacg gggccgtcgg ccgaaggcca aacattagcg gtttgcccga gattctctaa
     1921 ttttcagaca gagaatctcg ggcacattat ctttgcgcaa aatttcgcat gaaaacgtcc
     1981 ctctaccttc tggcggcggc cacattcctc ttttccgcct gttcctccaa taatgtcacg
     2041 gtcgatgaca gtctcggaca ttatttcgac tccgcagggg taaaaggtac tttcgcgctc
     2101 ctcgacaacg gccagggacg ttttaccatc tataaccttc cgcgctatcg cgacagccta
     2161 taccctgcgg cccagacctt tgacatcctt cagacgctgg tggccttcca gaccggtatc
     2221 cttccggatg aaaaaaagac cgcgatcgtg gaagacagtt ccaccgctga tcctctcagt
     2281 cccggtaatc ccatcgattc gaagacaacg gttacctatg cctgggccat gcgggatccc
     2341 ggacctgtcg gcgatagggt ataccggcag ctggcggact ccatcggcat cgatacgctg
     2401 aaaaaatggg tcgattcgct tcaatacggc gataaagata ttaaagccga ctccggccgg
     2461 gtcctgattc gcggcatcag gatcaatgcc gaccaacaac tcggactggt caaaaagctt
     2521 tatttcaacc agctgccctt tttccggcga ccccaggatc tcgtcaaagg catgctgacg
     2581 acggaagtca actccaatta caagctggtc tacaaaaccg ggcgcgccgc atataacccc
     2641 ggccccgcca tcggatgggt gatcggctgg gtccaggaga acgaacatcc ctatttcttc
     2701 gtgatcaatc tggaaagccc cgatggacac tcggatgtag aaaccattgg tattcaactc
     2761 gctaaaaaga tacttccgaa actcggtttc ttccagggaa agaaataatt ggcgtttttt
     2821 ttgcattttt catgacgccc gtttaagggc gaaaaaccta tcttgcaaac gtgtttcgaa
     2881 tcgaacatat cgaatatctc gtcggtcttg cggcaatccc actcctgctg gcgacccttt
     2941 attttttgtt atcctggaag aaaaaaacag ccggccgcat cggcgatccg gtcctcgtcc
     3001 ggcaactgat ccggaatttt tcatcctggc gcttcctgct caaggcggct ctggccctcc
     3061 tggccttcat tgtcatcgta ctgggagccg gcaatccgca gaaacccggc tcgatgcaga
     3121 acatccagag aaaaggggtg gatgtcatgt tcgtactcga tgtcagcaaa agcatgctgg
     3181 caagagatat caaacccagc cgtctggaaa aggccaaaca gctgatgctt cagcttgtcg
     3241 ataaattgga caacgaccgc atcggactgg tcctttttgc cggtaggccc tatatgcaga
     3301 tgccgcttac gaccgatcac ggcgcctcta aaatgtatat ccaacaggcc agccccgacg
     3361 ccgtccccac ccagggaacc gtcattggcg aggctttaca gatggcaaac ggcgcatttg
     3421 acgcccggga tacgaaatac aaatccatca tcctgatatc cgatggcgaa gaccacgacc
     3481 cggacgcccg gaagatagcc aaaaagctgg cacaggacgg ggtcatgatc aataccgtcg
     3541 gcatcggtac gccggacgga tctcccatcg tggatcccgc gacgggcgag ctaaagaaag
     3601 acgatcaggg aaatgtcgtc atgtccaaac tgaatgaggc cgagctgcaa cagctggcga
     3661 cctccaccaa cggacaatat atccggctcg acaatgtaga cgatgcactg atcaccatga
     3721 gccaacagct cgacaatgcc gggaaaaaat cggtgacaga cgccgagctc atcgattaca
     3781 aaagtttttt ccaatggttt ctcgctgtgg ccctggtact gctggtggtc gagtacctgg
     3841 tgtccgaacg gcggaaaaaa gttcctgccg tcgtccctgc actttttctt ttggccctcg
     3901 gatgcgctac actacccgct tccgcccaaa gcaccaacgc ccggatcaga agtggcaacc
     3961 ggtattataa gaagca
//
LOCUS       DASYSV010000579         4032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_241899, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000579 DASYSV010000000
VERSION     DASYSV010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241899"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1266
                     /gene="rocD"
                     /locus_tag="VGQ51_18060"
     CDS             34..1266
                     /gene="rocD"
                     /locus_tag="VGQ51_18060"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEV9038549.1"
                     /translation="MNTSASEEVLHYLGLEEKYSAHNYHPLPVVLTRGSGVHVWDVNG
                     RQYFDFLSGYSAINQGHCHPRIIAALTEQAHQLTLTSRAFHSDLLGEYARYITEYFGY
                     AKVLPVNTGVEAVETAIKLCRRWGYQIKGIPEDQAKIIVCAGNFHGRTTTVISFSTDA
                     DSNRQFGPYAPGFECIPYNDTTSLARALEDPNVAGFLVEPIQGEAGVIVPDDGYLRAA
                     RKYCNDARVLFIADEIQTGLGRTGRMLACDHEAVRPDILILGKALGGGVLPVSAVLAD
                     DEIMLTIRPGEHGSTFGGNPLACRVAMTALDVLKEERLDENAATLGELFRDRLSSLRS
                     PYIDTVRGKGLLNAIVIRHPHPDAAWELCLALKDNGLLAKPTHGDKIRLAPPLIITRE
                     QILECVDIIQYSLDRLSR"
     gene            1286..2002
                     /locus_tag="VGQ51_18065"
     CDS             1286..2002
                     /locus_tag="VGQ51_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEV9038550.1"
                     /translation="MHQVTASPLHEEQHMRGSEILTDIVIGMSDGLTVPFALAAGLSG
                     AVGPAHLNLIWIAGLAEIAAGSIAMGLGGYLAGKTEMDHYNSELRREYDEVDRVPDRE
                     RDEVREFFSGLGLSKDIQDKAVDEMTKDTDKWVDFMMKYELGLDKPDPQRARKSAFNI
                     GLSYVVGGLIPLSPYFFVTDGITGLKISAVITLCCLFIFGFFKSRLTGVNPISGAIRV
                     MVIGALAAGCAFAVARLIQA"
     gene            complement(2035..2445)
                     /locus_tag="VGQ51_18070"
     CDS             complement(2035..2445)
                     /locus_tag="VGQ51_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038551.1"
                     /translation="MKNSQWIGIAGALLMIGACFLPWAYYPDLQKEFTGFFSEQNIYG
                     RPGKVFAFFAVIDIALFLIPRVWAKRANILVAAMAFAFGVKSYILYTACYRGICPERR
                     AGIFLVVSGAVIVLIASLLPDLPVKEKEGRVKEP"
     gene            2682..3017
                     /locus_tag="VGQ51_18075"
     CDS             2682..3017
                     /locus_tag="VGQ51_18075"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV9038552.1"
                     /translation="MNQFLIIVRGSHASAGSPDQVQGRRVAFNEWVAKVLDGRFVGGA
                     PLEDNDARLLTSKGQFVTDGPFVESKEMISGYMIIKAKDIDEATRLTDECPLLKDFRL
                     EVRKLMPME"
     gene            3028..3558
                     /locus_tag="VGQ51_18080"
     CDS             3028..3558
                     /locus_tag="VGQ51_18080"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEV9038553.1"
                     /translation="MSEISGNLQQLVDHLFRHQSGRMVAILTRLFGLHNLELAEDVLQ
                     DTLHEALRTWGTNGIPDNPEGWLMTVAKRKAINTIKRERFYRSFAGDMDTLLKSEWTA
                     SYTMDQVFLEDEIRDSQLRMIFTCCHSGAACGGTGRIDAQNPLRIRHDGDQRGPPHHR
                     SQCSKTDLPRQRKDPE"
     gene            3443..>4032
                     /locus_tag="VGQ51_18085"
     CDS             3443..>4032
                     /locus_tag="VGQ51_18085"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEV9038554.1"
                     /translation="MTLKTLCGFGTTEISAALLTTEANVQKRIYRAKEKIRSESIDFT
                     VPTGKDLRERLDVVLLVIYLLFNEGYSATGDNPVLRKDLCLEAMRLGMLLTEHPATGG
                     YPPLHALMALLCLHAARFEARIDPNNSLVILQDQDRNTWDPALIARGMQFLTQSATGD
                     SATAYHLEAAIAAEHCLAPDFAFTDWERIHSYYTALV"
BASE COUNT          921 a         1199 c         1071 g          841 t
ORIGIN      
        1 attttatcaa ctttatctca cttttttatg ccaatgaaca cgtcggcctc ggaggaggtg
       61 ttacattatc tgggactgga agaaaaatat agcgcccaca attaccatcc gctacctgtc
      121 gtcctcacca ggggttccgg cgtacatgtc tgggacgtca acggccgtca atatttcgat
      181 ttcctcagcg gatactcggc gatcaatcag ggacattgtc atccccggat catcgcggct
      241 ctgaccgaac aggctcatca gctcacactc acgtcgcggg cctttcacag cgatctgctc
      301 ggggagtatg cccggtatat cacggagtat ttcggctatg ccaaggtcct acccgtcaac
      361 accggtgtgg aagcggtcga aacggccatc aagctttgcc gtcgctgggg ctatcagata
      421 aagggtatcc cggaagacca ggcgaagatc atcgtctgcg ccgggaattt tcacggacgc
      481 acgacaacgg tcatttcatt cagcaccgat gccgactcca accggcaatt cggtccctat
      541 gcccccggtt tcgaatgcat cccttacaac gatacgacct cgctggcccg tgcgctggaa
      601 gaccccaatg tagcaggctt cctggtggag cccatacagg gggaggccgg ggtgatcgtt
      661 cccgacgacg gctatctccg ggctgcccga aaatattgta acgatgcacg ggtcctcttc
      721 atcgccgatg agatccaaac ggggttaggc cgtacggggc gaatgctcgc ctgcgatcat
      781 gaagccgtgc gacccgacat ccttatcctc ggaaaagcgc tgggaggagg agtactaccg
      841 gtatccgcgg tgctggccga cgacgagatc atgctcacga tccggccggg agaacatggt
      901 tctacattcg gaggcaaccc gctggcatgt cgcgtcgcga tgaccgcgct ggatgtattg
      961 aaagaagaac gtctcgacga gaacgccgcc acattgggag aactcttccg cgacaggttg
     1021 agctcccttc gttcgccata tatagatacc gttcgcggca agggtctgct gaatgccatc
     1081 gttatccgtc atccacaccc cgatgcggca tgggaacttt gcctggcatt aaaggacaac
     1141 ggtctgctgg cgaaaccgac acacggcgat aagatccggc tcgctcctcc gctgatcatc
     1201 acgcgggagc agatcctgga atgcgtggat attattcaat atagcctcga ccgcctatcc
     1261 cgataaattt ccatagctta gcggcatgca ccaggtaact gcgtccccac tccatgaaga
     1321 gcagcatatg cgaggctcgg agatcctaac ggatatcgtc atcggcatgt cggacggcct
     1381 tacggtcccc ttcgctctgg cggcaggcct tagcggcgca gtaggtcctg cacatctcaa
     1441 tctcatctgg atcgccggtc tggcggagat cgcggccggc tctatcgcca tgggactggg
     1501 gggctatctg gcgggtaaga cggaaatgga ccattacaat tccgagctgc ggcgggaata
     1561 cgatgaggtg gacagggtgc ccgaccgcga acgcgacgaa gtacgggaat tcttttccgg
     1621 tctcggcctc agcaaggaca tacaggacaa ggcggtggat gaaatgacca aagacacaga
     1681 caaatgggtc gatttcatga tgaagtatga actcgggctg gacaaaccgg atccccaacg
     1741 tgcacgcaaa agcgctttta acatcggtct ctcgtatgtc gtcggcggcc tgatcccgct
     1801 aagcccctac ttcttcgtca ccgacgggat cacgggtctg aagatctcgg cggtcattac
     1861 cttatgctgt ctgttcatct tcgggttctt caagagcagg ctcaccggcg tcaaccctat
     1921 cagcggcgcc atccgggtca tggtcatcgg ggcactggcc gccggctgcg cattcgccgt
     1981 ggcaagactc atccaggctt agtccctgac ctgtctttgc gctgctgagc cggatcacgg
     2041 ctccttcacc cttccctcct tctccttcac cggcaggtcg ggcagaaggc tggcgatcag
     2101 gacgatcacg gctcccgaca ccaccaggaa gatacccgcc cggcgttccg gacagatacc
     2161 gcgataacag gcagtatata agatatacga cttgacgccg aacgcgaagg ccatcgcggc
     2221 caccaggata ttggcccttt tggcccagac acgcggaatc aggaacaggg cgatatcgat
     2281 gacagcgaaa aatgcaaaga ccttccccgg acgcccatag atattctgct ccgaaaaaaa
     2341 tcccgtgaat tccttctgca gatcgggata atatgcccat ggcagaaaac aggccccgat
     2401 catcagcagc gctcccgcaa taccgatcca ttgcgaattt ttcatgtctc taatttgcga
     2461 ttataccttc catccggcgg gcccctgcta tccattgacc atgacgacat gggctaaagt
     2521 ctggccttcg gccaaaagac ctccggtctt ttcatgcggc gaagatagcc attccctaac
     2581 ttcgatccgg agagggtgcg gacaaaaaat attccagccc ggttgtccta ttctttccag
     2641 cccgattgac aagggatcat tcatcatcaa aaaacatttt tatgaatcag tttctgatca
     2701 ttgttagagg ttcgcacgca tcggccggga gccccgacca ggtgcaggga agaagggtcg
     2761 cattcaacga atgggtggcc aaagtgctcg acggccgttt tgtcggcggc gccccgctgg
     2821 aagataacga cgcccgtctg ctcacctcca aagggcagtt cgtcaccgac ggccctttcg
     2881 tggaatccaa ggaaatgatc tccggctaca tgatcatcaa ggcaaaagac atcgacgaag
     2941 caacccggct caccgacgaa tgcccgctgc tcaaggactt ccggctggaa gtgcgcaagc
     3001 tgatgcccat ggaataaacc ttatcgcatg tccgaaatat ccggcaatct acagcaattg
     3061 gtcgaccatc ttttccggca tcagtcggga cggatggtcg ctattttgac gcgtcttttc
     3121 ggccttcaca atctcgagct ggccgaagac gtgctgcagg acacgctgca cgaggctctg
     3181 cgcacctggg gaaccaacgg tatccccgac aaccccgaag gctggctgat gacagtcgcc
     3241 aaacgcaagg ctattaacac catcaaacgc gaaagattct accggagctt tgccggggac
     3301 atggacacgc tactcaaatc ggaatggacc gcctcctata ctatggacca ggtctttctc
     3361 gaagacgaga taagggatag ccagctgcgc atgatcttca cctgttgtca ttccggcgct
     3421 gcctgcggag gcacgggtcg cattgacgct caaaaccctc tgcggattcg gcacgacgga
     3481 gatcagcgcg gccctcctca ccaccgaagc caatgttcaa aaacggatct accgcgccaa
     3541 agaaaagatc cggagtgaaa gcatagactt caccgtcccg accggtaaag atcttcgcga
     3601 gcggctcgat gtcgtgctgc tggtcatcta tctcctgttc aatgaaggtt acagcgccac
     3661 cggtgacaat cccgttctgc gaaaagacct ctgtctcgaa gcgatgcgtc tcgggatgct
     3721 gctgaccgag catccggcta ccggtggcta cccaccgctc catgcactga tggcattgct
     3781 atgtcttcat gccgcacgtt tcgaagcgcg tattgacccc aataactccc tggtgatcct
     3841 gcaggaccag gatcgcaaca cctgggaccc ggcgctgatc gcacgcggca tgcaattcct
     3901 cacacaatct gcaacgggag actccgccac ggcctatcat ctcgaagccg ccatcgcggc
     3961 agagcattgt ctcgccccgg atttcgcttt cacggattgg gagaggatcc atagctatta
     4021 taccgccctc gt
//
LOCUS       DASYSV010000580         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1525341, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000580 DASYSV010000000
VERSION     DASYSV010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525341"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VGQ51_18090"
     CDS             <1..657
                     /locus_tag="VGQ51_18090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9038555.1"
                     /translation="GIGIPEASIPFLFEHFSQAGLDISRRYGGSGLGLAICQQLLQLQ
                     GGEITVSSREQEGTTFQFRLSYGYACGSDVQTSVPTAAVGQANLQSLAGKRFLVVEDN
                     EVNQLLIDQVLRRGGGEVRLAGNGEEAIGLLQGGESYDLIIMDLQMPVMDGYAATKYI
                     RQELRLSIPIIAMTATAMVDEQLRCFEVGMSDYMTKPFEFADLYRRINNQLTPVFRLL
                     "
     gene            691..1215
                     /locus_tag="VGQ51_18095"
     CDS             691..1215
                     /locus_tag="VGQ51_18095"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV9038556.1"
                     /translation="MLALYIVLGVIALFLLVAALAGTGWHYEQSVLIQAPADKVWGQI
                     STLRAINQWSPWMDRDPHIKLSYTDKDGAPGAGFSWDSPVKNVGAGSQTILKVADRSE
                     VSTRVDFFRPFKGTGLANFRMSCENAGVRVTWSMKSSLPYPMNIVKVFGGIRRNMGRD
                     FTKGLNKLKSLCES"
     gene            1294..1677
                     /locus_tag="VGQ51_18100"
     CDS             1294..1677
                     /locus_tag="VGQ51_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038557.1"
                     /translation="MKQSPAISILLLVFLCLLGLLVFSATIFGQARSVDRDTTAALLS
                     ATVYPEPVGGTMNVRIDGTHANEDLLFQLVRVNGDVIYSRNIHAVGATTVKTLRRPMV
                     DPGYYVFRVVRQRNGEVAQGRITFK"
     gene            complement(1687..2562)
                     /locus_tag="VGQ51_18105"
     CDS             complement(1687..2562)
                     /locus_tag="VGQ51_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9038558.1"
                     /translation="MKNYSVTAIAAFLFLTACVSNKKFKSMQQQEEAKYNTLNASYNT
                     LQGDLKNCNDAKADLATQFGTLKDQNADLLRQVDLLKQNNTTALKQLQDLSVISASQA
                     ESIKKSLDNIGAKDVYIQDLQKEMARKDSLNMALVMNLKGAVGDLDDKDINIKVDKGV
                     VFIDISDKLLFKSGKYDITDQAKEVLGKVALVLKAQPHIEFMVEGHTDNIPYKRPPLL
                     DNWDLSVKRATAVVRILQNKYGLAPEHITAAGRSEYVSVASNDTPEGRAANRRTRIVI
                     LPQLDQFFQLLEKKD"
     gene            complement(2618..3871)
                     /locus_tag="VGQ51_18110"
     CDS             complement(2618..3871)
                     /locus_tag="VGQ51_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEV9038559.1"
                     /translation="MQTSKKAAMGFIFVTLFIDCMGWGLIIPVMSRLIVELKHVPANE
                     ASKYGSYLLFAFAGAQFICSSIVGNLSDRFGRRPVLLSSLLGFGVDYIILAIAPTYGW
                     LFIGRIVAGMTGASFTTANSYIADISANPAERAKNYGMVGAAFGLGFMLGPALGGVLS
                     HWGIRAPFYGAAVLCLLNCLYGYFLLPESLSKENRRAFDWKRANPFGSLKFLMRHPEI
                     GNLSFAFFLIYLAVQSVQANWNFFTDYRFHWGPGMIGISLTVVGVLIAGVQAGLTRVV
                     NPRLGNEKSIYLGLSLYTLGLILFAFASQSWMMFVFLVPYCLGGIAGPSLQAVISGHV
                     QSTNQGELQGALTSLMSVTTIIGPLIMNNSFAYFTSSKAPFTFPGIHFLIGAVCMLMS
                     VIIAYRVLSRDKKHAVVNPAVKSAG"
     gene            complement(4019..4432)
                     /locus_tag="VGQ51_18115"
     CDS             complement(4019..4432)
                     /locus_tag="VGQ51_18115"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV9038560.1"
                     /translation="MNQRTPSPRIPKTRLVIYWILTGYLAFESILSATWDFDWLNKGF
                     AIGMMQHLGYPTYFLVIKGIGTLLAAPVFVLPGLGLLKEWAYFGTFMIYTGAIASHLA
                     IGDGFKDMAPPIAFLAITIASWALRPASRRSGATF"
BASE COUNT         1038 a         1114 c         1295 g         1058 t
ORIGIN      
        1 gggatcggca taccggaggc cagcatccct ttcctgttcg aacatttttc acaggcgggt
       61 ctggacatat cgcggagata tggcgggtcg gggctcggtt tggccatctg tcagcagtta
      121 ctgcaattac agggcggtga gatcacggtc agcagtcgcg aacaggaagg cactactttc
      181 cagttccggc tgagctatgg atatgcctgc gggtccgatg tgcagacatc cgtcccaacc
      241 gcggcggtgg gtcaggccaa tctgcaaagc ctggcgggaa aacgatttct cgtggtggag
      301 gataatgagg tcaatcaatt attgatcgat caggtgctcc gcagaggcgg gggcgaggtc
      361 cggcttgcgg gcaatgggga agaagcaatc ggtcttctgc aggggggcga aagctatgac
      421 ctgatcatta tggatctcca gatgccggtc atggacggtt atgccgcgac gaaatatatc
      481 aggcaggagc tgcggctttc catacccatc atcgccatga cggcgaccgc catggtggac
      541 gaacagctgc gttgttttga ggtcgggatg agcgactata tgaccaagcc gttcgaattc
      601 gccgatctgt acagaaggat caacaaccag ctgacgccgg tgttccggtt attatgacaa
      661 tttattatat ttattgtcaa aacacctgct atgcttgctt tatacattgt attgggcgtt
      721 atcgcgctct tcctgttggt agctgcgctt gccgggactg gctggcacta cgaacaatcc
      781 gttctcattc aggctccggc cgataaggtc tggggacaga tcagcaccct tcgggccatc
      841 aatcaatgga gcccgtggat ggaccgggac cctcatatca aattgtccta tacggacaaa
      901 gatggcgcac ccggcgccgg tttttcctgg gacagccctg ttaagaatgt gggagccgga
      961 agtcagacca ttctcaaagt ggcggatcgg agtgaggtgt cgacgcgggt cgatttcttc
     1021 cggccgttta aaggaacggg gctggccaat tttcggatgt cctgtgaaaa tgcgggggtc
     1081 cgggtcacct ggagcatgaa gagctcctta ccgtatccca tgaatatcgt caaggtattc
     1141 ggggggatca ggcggaacat gggccgcgac ttcactaaag gattgaataa gctgaagagc
     1201 ctctgcgaga gctgagaaga cgatttgcca gaatttataa gattgggccg atcgcgccat
     1261 tttgcgcgcg attaaaaggt aacttttggg tgtatgaaac aatcacccgc catttcgatc
     1321 ttgttgctgg tgttcctttg cctgcttggg ctgttggtat tttctgccac tattttcggg
     1381 caggcccgat cggttgaccg cgatacgact gctgcgctgc tctcggcgac ggtatatcca
     1441 gagccggtgg gaggcaccat gaacgtcagg atcgatggga cccatgccaa tgaagacctc
     1501 ctctttcaat tggtacgcgt caatggggac gtcatttaca gcaggaatat ccatgcggtc
     1561 ggggccacga cggtcaagac cctccgtcgc ccgatggtgg atccgggcta ctatgttttc
     1621 cgcgtagtcc gtcagcgcaa cggcgaggtg gcgcaggggc ggatcacctt taagtaattt
     1681 ctgtggttaa tcctttttct ccaggagttg gaagaactga tccagctgcg gaagaatgac
     1741 gatacgggta cgtcggttgg cggcccggcc ttccggcgta tcattggagg ctaccgaaac
     1801 atattcgctg cggcccgccg cggttatatg ctcgggcgcc agaccgtatt tgttttgcag
     1861 gatacgtacg acggccgtag cacgctttac gctcaggtcc cagttgtcga gaaggggtgg
     1921 gcgtttgtag gggatgttgt cggtatggcc ttccaccatg aattcgatat gtggttgggc
     1981 cttcagcacc agcgccactt ttcccaatac ttcctttgcc tggtcggtga tgtcgtattt
     2041 accgcttttg aacaatagct tgtcggagat atcgataaaa acgacaccct tatcgacctt
     2101 gatattgatg tccttgtcat cgaggtcgcc gaccgcgccc ttgaggttca tcaccaatgc
     2161 catgttgagc gaatctttgc gcgccatttc cttttgcagg tcctgtatat aaacgtcttt
     2221 ggcgccgatg ttgtcgagcg actttttgat gctttccgcc tgggacgctg aaatgaccga
     2281 aaggtcctgc aattgtttga gcgccgtggt attgttttgt ttcagcagat ccacctgtct
     2341 cagcagatcg gcgttctgat ccttcaaagt gccgaattgc gttgccagat cggctttggc
     2401 gtcattacaa ttcttaaggt cgccctgaag ggtgttatag gatgcgttga gggtattgta
     2461 tttggcctct tcctgctgtt gcatgctttt gaatttctta ttgctgacgc atgctgtaag
     2521 aaaaagaaaa gcggcgatcg cggtgactga ataatttttc atgccccaaa agtactcgca
     2581 gctgtctttc gtacggctgt ttaacggtta aacgccgtca gccggccgat tttaccgccg
     2641 ggttcacgac ggcgtgtttc ttatcgcggc ttagcacccg gtaggcaatg atgacgctca
     2701 tcagcataca gacggcaccg atgaggaagt ggatacccgg gaacgtgaag ggcgccttgc
     2761 tgctcgtgaa ataggcaaag ctgttgttca tgatcagcgg acctatgatc gtcgtcacgc
     2821 tcataagact ggtcagtgcg ccctgaagct cgccctggtt ggtggactga acatggccgg
     2881 aaataacggc ttgcagcgaa ggacccgcga tgccgccgag acagtaaggg acaaggaata
     2941 cgaacatcat ccagctttgg ctggcaaatg cgaacaggat cagtccgagc gtatagagcg
     3001 acagaccgag atagatgctc ttttcattac ccagtcgagg attgacgacg cgggtaagcc
     3061 cggcctgaac tccggcgatc agcacgccga cgaccgtcag cgagatcccg atcatgccgg
     3121 gtccccagtg aaaacgatag tcggtgaaga agttccagtt ggcctgcacc gactggaccg
     3181 caagatagat caggaaaaaa gcgaacgaaa gattgccgat ctcgggatga cgcatcagga
     3241 atttcaagga gccgaagggg ttcgcacgtt tccagtcgaa ggcgcggcga ttctctttgc
     3301 tgagtgattc cggcagcagg aaatagccat ataggcagtt cagcagacaa aggacggcgg
     3361 ctccatagaa aggcgcgcgg ataccccagt gtgaaaggac tccgccgagc gcgggaccca
     3421 acataaatcc gagaccgaat gccgccccga ccattccgta gtttttcgca cgttctgccg
     3481 gatttgcgct gatatctgcg atataagaat tagctgtggt aaaactggcc cctgtcatgc
     3541 ccgctacgat gcggccgatg aacaaccagc catatgtagg ggcaatcgcg agaatgatat
     3601 agtcgacgcc aaagcccagc agcgacgaca gcagcacggg acgacgtcca aaccgatcgc
     3661 tgagattacc tacgatggag gagcagatga actgcgcacc ggcaaatgcg aacaataaat
     3721 agctgccgta tttgctagcc tcattggccg ggacatgttt caactcgacg atcagacggg
     3781 acatcaccgg tatgatcagc ccccagccca tacaatcgat gaacaaagtg acgaaaataa
     3841 agcccatcgc ggccttttta gatgtctgca taataagcgg ttggttggcg gttggttagc
     3901 gaatctaaca aacttacgtt taaatccgaa ttcccgggag ggatgcgaat ctttttctat
     3961 tttgccgcgt gggccagatc cctgcccggc tgtttgggga tgggtttcga ccgcccgttc
     4021 aaaaggttgc cccggatctt cgcgaggcag gacgcagggc ccaggaggcg atagtaatgg
     4081 caaggaatgc gataggcggc gccatgtctt taaagccgtc cccaatggcc agatgagagg
     4141 cgatggctcc cgtatagatc ataaacgtcc cgaaatacgc ccattctttt agtaaaccca
     4201 gaccggggag gacaaaaacg ggcgcggcga gcagtgtgcc gatgcctttg atgaccagaa
     4261 aataggtagg atagccgagg tgttgcatca tcccgatggc gaatcctttg ttgagccagt
     4321 cgaaatccca tgtggcgctg aggatggatt caaaggcaag atagccggtc agtatccagt
     4381 aaatgaccaa ccgggttttc gggatcctcg gagagggtgt acgctgattc atggtgtttg
     4441 tttaatcaat aagcattgga tattgcaaaa ttggacatgt ttcccgaaga attcataatc
     4501 catgc
//
LOCUS       DASYSV010000581         7860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_757766, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000581 DASYSV010000000
VERSION     DASYSV010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757766"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VGQ51_18120"
     CDS             complement(<1..590)
                     /locus_tag="VGQ51_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038561.1"
                     /translation="MRVNIILTVLFSAWLQAGAQTAVTPPFPRLATIYSKTGAGVSQR
                     TDEGKRVIAKFDLYIPTMNMWGGPCGNDSSCRGHAGMTIGQYLKFLNPHQMDLIYVHS
                     AFFPRWSAPRDGRGYIVGDTSYYFDLRWLLTYAGTIMHGDVDPLIRNIPVAELSPFAI
                     GDFVLIGGIGRQNTELVKVTSKSATDGQGELTVERA"
     gene            complement(587..2023)
                     /gene="uxaC"
                     /locus_tag="VGQ51_18125"
     CDS             complement(587..2023)
                     /gene="uxaC"
                     /locus_tag="VGQ51_18125"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEV9038562.1"
                     /translation="MTFMDDNFLLQGTTAQRLYHEYAKDMPILDYHCHLPPDQIAGDR
                     NFLNLTQAWLYGDHYKWRAMRTNGIDERYCTGDASDREKFDKWAATVPYTLRNPLYHW
                     THLELQRYFGVHRTLGPATAGAIYDECSEMLRSPGFSVKNLLRKMNVAAICTTDDPVD
                     ALESHVTLNKDGFEIPVLPAFRPDRAMDVRDATAFNGYLLRLEEVTNFSISSWNDFLY
                     ALQHRHDYFASKGCGISDHGLEVIDTEEFTGFEIDAIFNKVRAGRQLNGSEQRKFRSA
                     MLLHLAEWDWEKDWVQQYHLGALRSNNTRGRLQTGADSGWDSIGDFQQARSLATFLDR
                     LDKEGRLARTILYNLNPADNELMATMIGNFNDGSIAGKVQFGAAWWFLDQKDGMIKQM
                     NALSNMSLLSRFVGMLTDSRSFLSFPRHEYFRRILCNLFGSEIDGGELPHDMEWIGKV
                     IRDICYYNARSYFNWEGIVRPQQLKIVQ"
     gene            complement(2041..2805)
                     /locus_tag="VGQ51_18130"
     CDS             complement(2041..2805)
                     /locus_tag="VGQ51_18130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV9038563.1"
                     /translation="MDTNKAFSLTGRRAVITGGGTGIGLGISRALGDAGASLVLIGRR
                     ESVLQKAAAELGGDCRYIPYDINDRKGLPDLINRIGMEFGPIDILVNNAGAHLKKPSL
                     ETTDEEFDHIIRTNLLSGFTITRECVKHMEQRGAGVILFISSMTGLFGMDKVAAYGTS
                     KTAIIGLMRAMVMEYSAFRVRVNAIAPGWIESDMLNEALNNDKERKEKIINRIPYRSF
                     GTGEDIGAAAVYLCSDAARYVTGVVLPVDGGAAYSF"
     gene            complement(2827..3828)
                     /locus_tag="VGQ51_18135"
     CDS             complement(2827..3828)
                     /locus_tag="VGQ51_18135"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV9038564.1"
                     /translation="MDLKINPDSNIPKYQQIADGLIGLIEAGVLKVGDKAPSIDTISK
                     NNKVAKETVVQAYRHLKSNGIIESSEKRGFYIQTNVTNKRWRVLVLFNVMNPSKEIIY
                     KSIVNSLGDRAHVELLFHNYNFEMFENIVKSKANMFHYYIIMPHFNPDSHKVLELIPP
                     EKLLVLDRPLLHPIAGASAVYQDFRADVFDALLSQKERIRRYKQIVILFPKGKGLPTE
                     IADGIKLFGTKNKMPVQVAAEVQPKMLVPDTLFITLTDEILIDLIELQQQSKLRIGKG
                     IGIISYNENPLKRILAGGIATLTSDFEKMGRTAAECIINKSTIVTRNPFYLILRNSL"
     gene            complement(3912..5474)
                     /locus_tag="VGQ51_18140"
     CDS             complement(3912..5474)
                     /locus_tag="VGQ51_18140"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEV9038565.1"
                     /translation="MNFFFALSLGVLTVLPGGTNAQAPGNRLPAVARPHFKTDTFNIG
                     KYGAISDGITLNTKAINEAIDACSKKGGGVVLVPGGFWLTGPIVLKNNVNLSVRGDAL
                     LLFTRDFDQYPLIQSYYAGLPSVRCQSLISGSDLTNVAITGEGVINGSGDAWRPSLQQ
                     KMSVSEWNHLVASGGVLSTDPKRPKTWYPSEKALLGSEVLTDSGTLRPGVTFPGYPAI
                     KDFQRPTLLSMYHCRKVLLEGVTFQNSPNWNLHFLLCQDITIDKVHSKNPPFGQNTDA
                     LDLESCKKALVENSTVEGGDDGITLKSGRDSSGRARNVPTEDVIIRNCIAYNVGSGVA
                     IGSEMSGGVRNIFISHCSFVGSSLGIKLKTKRGRGGVVEKIYASDINMSDIQQYALVF
                     DMFYGVIGAPKIGTQATTNPQLNADPVTDATPTFRDIHFRHIYCNGAEQAIFVRGLPE
                     MPVKDVYLEDMVFKSKKGMECMEARGVHLKNIRFITTDMDPVIHLQDSKDITLDGKRF
                     DADAKPTAVRLQ"
     gene            complement(5494..7539)
                     /locus_tag="VGQ51_18145"
     CDS             complement(5494..7539)
                     /locus_tag="VGQ51_18145"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV9038566.1"
                     /translation="MYTKHLSMGTLRLGAIPKLALTSLIILFICPISNAQRQYNVFES
                     AWWPFNTVHTYLEQAAFNQWEARRTDLRKALTSPSAMRTYRDNCRKRYLEILGTLPAR
                     TPLYPQITGTLSETGYRIEKVVYQSMPGHHVTANVYIPQGHGPFPGVLLFCGHSLNGK
                     EDASYQRTAILFATHGFVVLVADPISQGERYQLTDEKGHPIIALGTTEHTLFNAGATL
                     LGTGTTAYELWDNVRGLDYLCSRGDVDTSRIGCLGTSGGGTQTTYFAGFDPRIKVSAP
                     CSYVATRERNFNLPLIGASDGCQHIPGEGEARLEIDDFLVMAAPKPLLVLAARFDFVD
                     EVGVEIASKELQQVYSALGHPDRYKLFVYDDGHSIAKPKREAAVTWFRKWLCKDSTAV
                     KEGDIAILPDSTLFCTSTGEVNTAFPNERTLKDIDLSLADRYKADRETFAGRADNRTI
                     IRHLLHLHANAFSDPINVEWMGESSGPVQFKKCLVRKKGEIPLPCLVATPQGPAKNVL
                     VWVDEEGKAKVIKDSLPAIQEALQQGSMVVLADLRATGETLDAPRPNDTKYNYNIEYR
                     NSMTALHVGRSLPGQQVADMITLTDFITKPEGLGMTGLPIRVHASGGNAAVALHHLVL
                     DHRITRIHLYHTIRSYYDLLHDPTQKDQYRYVIPGILKYYDLPQLVELAGADKVFYQ"
BASE COUNT         1636 a         2047 c         2215 g         1962 t
ORIGIN      
        1 tgcgcccttt caaccgtcaa ttctccctgg ccgtcggtgg cagatttcga ggttacttta
       61 acgagttccg tgttctgccg tcctatgccg ccgatgagta cgaaatcgcc gatggcaaaa
      121 ggagatagtt ccgctaccgg aatatttcgg atcaggggat cgacatcccc gtgcattatg
      181 gtgccggcat aggtgagcag ccaccggaga tcgaagtagt aagaggtatc gccgacgatg
      241 tatcccctgc cgtcacgggg agcgctccat cggggaaaaa acgcgctgtg tacatagatc
      301 aggtccatct gatgcgggtt caggaatttg aggtattgac cgatggtcat gccggcatga
      361 ccacggcagc tgctgtcatt tccgcagggg ccgccccaca tattcatagt gggtatatag
      421 agatcgaatt tcgcgatgac ccgcttgcct tcatcagtgc gttgcgacac gcctgcgccg
      481 gtttttgagt aaatggtggc caggcgcggg aatggaggag tgaccgcggt ctgcgctccg
      541 gcctgcaacc aggcggaaaa caataccgtc aggatgatat tcactctcat tgtactattt
      601 tcaattgctg cggcctgaca ataccttccc aattgaaata actcctggcg ttgtaataac
      661 agatatccct gatcactttg ccgatccatt ccatatcgtg aggcaattca ccgccgtcta
      721 tctcgctgcc gaagaggtta cagaggatac ggcgaaaata ttcgtgacgc ggaaaggaga
      781 ggaagctgcg ggagtcggtc agcatgccca caaaccggct gagcaggctc atattggaca
      841 gcgcgttcat ctgtttgatc atgccgtcct tctgatcgag gaaccaccag gctgccccga
      901 actgcacctt acccgcgatg gagccgtcat tgaaatttcc gatcatggtc gccatcaatt
      961 cgttgtccgc cgggttgagg ttgtacagga tggtcctggc caaacggcct tctttgtcca
     1021 gcctgtcgag gaaggtcgcc agcgacctcg cctgctggaa atcaccgatc gagtcccagc
     1081 cggaatccgc tccggtctgc agccgtcccc gcgtattgtt gctacgcagg gcgccaaggt
     1141 gatactgttg gacccaatcc ttttcccagt cccattccgc caggtggagt agcatagccg
     1201 accggaattt gcgctgttcg gatccgttca gctggcggcc cgcgcgcacc ttattgaaga
     1261 tcgcatcgat ctcgaaaccc gtgaattcct ccgtgtcgat cacttcgagt ccgtggtcgc
     1321 tgatgccgca acctttcgag gcaaagtaat cgtggcggtg ttgcagcgca tataagaagt
     1381 cgttccagga ggagatcgag aagttggtta cttcttcgag ccgaagcagg tagccgttga
     1441 aggccgttgc gtccctgacg tccatggcgc ggtccggccg gaaagccggc aggacgggga
     1501 tctcgaaccc gtctttattc agcgtgacgt gggactccag agcgtccacg gggtcgtcgg
     1561 tcgtacagat ggcggcgacg ttcattttgc gcagcaggtt tttcacggag aatccgggtg
     1621 agcgtaacat ctccgaacat tcatcataga tggcgcccgc ggtggcggga ccgagcgtcc
     1681 ggtgcacgcc gaaatatcgt tgcagctcca ggtgggtcca gtggtacagc ggattacgaa
     1741 gggtgtaggg gacggtcgcc gcccatttgt cgaacttttc ccggtcggag gcgtccccgg
     1801 tgcagtaccg ttcgtcgatg ccgttggtgc gcatggcccg ccatttgtaa tgatcgccgt
     1861 atagccatgc ctgcgtgaga ttgagaaagt tccggtcgcc cgcgatctga tccggcggga
     1921 gatggcagtg ataatccagt atcggcatat ccttcgcata ctcatggtaa agcctttgcg
     1981 ccgtcgtgcc ctggagcagg aaattatcgt ccatgaaggt cattgcagta attttatttt
     2041 tcagaaagaa taggcggcgc ccccgtcgac cggcaatacg acaccggtga cataccgggc
     2101 ggcatccgaa cagagataaa cggccgcggc gcctatatct tcaccggtcc cgaagcttcg
     2161 gtaggggata cggttgatga tcttctcctt tcgttctttg tcgttgttca gcgcctcatt
     2221 gagcatatcc gactcgatcc agccgggcgc aatggcgttg accctgaccc ggaaggcgga
     2281 atattccatc accatagccc gcataagccc gatgatagcg gttttggaag taccgtacgc
     2341 ggctaccttg tccatgccga acagaccggt catgctgctg ataaagagga tcactccggc
     2401 gccgcgctgt tccatatgtt ttacacattc cctggtgatc gtaaatccgc ttagcagatt
     2461 ggtccggatg atatgatcga attcttcatc ggtggtttcg agggaaggtt ttttgagatg
     2521 cgcacccgcg ttgtttacca ggatatcgat gggaccgaat tccatcccga tcctgttgat
     2581 caggtccgga aggccttttc tgtcattgat gtcataaggg atgtaccggc agtcgccgcc
     2641 caattcggcg gcggcctttt gcagcaccga ttctcttcgg ccaatgagaa caagactcgc
     2701 cccggcgtcg cctaatgctc tgctgatacc cagccctata cccgtacctc cccctgtgat
     2761 caccgccctt cgacctgtca gtgaaaatgc cttgttagtg tccatatgat cggtaatgat
     2821 gtttgcttac aaagagtttc ttaagattaa ataaaatgga ttgcgggtaa cgatcgtgct
     2881 tttattgatg atgcactcgg ccgccgtcct gcccatcttc tcaaaatccg aagtgagcgt
     2941 agcgatcccg ccggccagga tccttttcaa cgggttttcg ttatagctga tgatgcctat
     3001 gcctttgccg atgcggagct tgctctgctg ctgcagctcg atcaggtcga tgaggatctc
     3061 gtcggtcagg gtaatgaata gcgtatcggg taccagcatt tttggctgga cctctgccgc
     3121 cacttgtacg ggcatcttat ttttggtacc gaacagcttt attccatcgg cgatctccgt
     3181 cggcaggcct ttccccttgg ggaaaaggat aacgatctgc ttatatctcc ggatcctttc
     3241 cttttgcgat agcagcgcat cgaaaacgtc ggccctgaag tcctggtaga cggcggaagc
     3301 gccggcaata gggtgcagca gcggtctgtc cagtacgagc agcttttcag gcggaatgag
     3361 ttcgagtacc ttatgcgagt ccggattgaa atgcggcatg atgatgtaat agtgaaacat
     3421 attcgccttg ctcttgacga tattctcgaa catctcgaaa ttgtaattgt ggaagagcag
     3481 ctctacatgg gcccggtcgc cgagggaatt gactatggat ttgtagatga tctctttcga
     3541 tgggttcatc acgttgaaca gaacgaggac tctccagcgc ttgttcgtga cattcgtttg
     3601 aatatagaat cctcttttct cgctcgattc tatgataccg ttagatttca gatggcgata
     3661 ggcctgtacc accgtttcct tggcgacttt gttgttcttg ctgatggtat cgatggaggg
     3721 tgccttgtcg cccaccttga gtacgccggc ttcgatgagc ccgatgagcc cgtcggcaat
     3781 ttgctggtat ttgggaatgt tcgagtcggg gttgatctta agatccatgg taaataaggt
     3841 tttcctgcaa aattagattc aatactatat tatactatac ttttttgtac aaattttaaa
     3901 aaatatccat tttactgcag acgtaccgcg gtgggtttcg cgtccgcgtc gaaccgcttc
     3961 ccgtcaagcg tgatgtcttt gctatcctgc aaatggatca cagggtccat gtcggtcgtg
     4021 ataaaccgga tattcttcag atggactccc ctggcttcca tgcattccat cccctttttg
     4081 gatttgaaga ccatatcctc gaggtaaacg tctttcaccg gcatttcggg tagtccgcgt
     4141 acgaatatcg cctgttccgc gccattgcaa taaatatgtc ggaaatggat atcccggaag
     4201 gtgggcgtcg catcggtgac ggggtcggcg ttcagttgtg gattggtggt tgcctgtgtt
     4261 ccgatcttcg gggcgccgat caccccgtag aacatgtcga agacgagggc gtattgctgg
     4321 atgtcgctca tattgatatc cgacgcatag atcttttcca ccacgccgcc gcgtccgcgt
     4381 ttagtcttca gcttgatccc cagactggag cccacgaaag agcaatgcga gatgaagata
     4441 ttgcgtacgc cgccggacat ttcgctgccg atggccacgc cgctgccgac gttataggcg
     4501 atgcaattgc ggatgatcac gtcctccgta ggcacattcc ttgcccggcc tgacgaatcc
     4561 cgcccggatt taagcgtgat gccgtcgtca ccgccttcga ctgtgctgtt ctccacgagc
     4621 gcctttttgc aagactcgag gtccagggca tcggtgttct gcccgaaagg aggattcttg
     4681 gaatggacct tatcgatagt gatatcctgg caaagcagga agtgcaggtt ccagttgggt
     4741 gaattttgaa aggtgactcc ttcgaggagg acctttcggc aatggtacat ggatagcagc
     4801 gtgggtcgct ggaagtcttt tatcgccggg tatccgggga aggtaacgcc cggtctcagc
     4861 gtgccgctgt ctgtcaatac ctcggaaccg agcagcgcct tttccgacgg gtaccaggtt
     4921 ttcggtcttt tcggatcggt gcttagaacg ccgccggagg ctacgaggtg gttccactcg
     4981 gaaaccgaca tcttttgctg cagcgacggc cgccaggcgt cgcccgaacc gttgatgacc
     5041 ccttccccgg tgatcgcgac gttggtcaga tcgctgccgg agatgaggga ctggcatcgg
     5101 acggatggca gacccgcata atagctttgg atgaggggat attgatcgaa atcccgggta
     5161 aagaggagga gggcgtcgcc tctaacggag aggttcacgt tattcttcag gacgatgggt
     5221 ccggtcagcc agaagccgcc cggcaccagc accactccgc ctcctttttt ggaacaggcg
     5281 tcgatagcct cattgatggc tttggtattg agggtaatgc cgtcgctgat cgcgccatat
     5341 ttcccgatat tgaatgtgtc cgtcttaaag tgcggtctgg cgacggccgg cagtctattg
     5401 cccggcgcct gtgcattcgt cccacctggt aagacagtca gcacacctaa ggaaagagca
     5461 aaaaagaagt tcattggcag ttgttagttg ggtttattgg tagaatactt tatccgcgcc
     5521 cgctagctcc accagttggg gcaggtcata atatttcaaa atgccgggta tgacgtagcg
     5581 atattggtct ttttgtgtag ggtcgtgtag cagatcgtaa taagaacgga tggtatgata
     5641 gagatggatc cgggtgatgc gatggtcgag gacaagatgg tgcaaggcca cagcggcatt
     5701 tccgccggaa gcatgtaccc ggatcggaag accggtcatg ccgagccctt ccggcttcgt
     5761 gatgaaatcg gtcagggtga tcatgtccgc cacctgttgg ccggggaggg accggccgac
     5821 gtgcaatgcc gtcattgaat tgcgatactc tatattgtaa ttgtactttg tgtcgttggg
     5881 tcgcggggcg tcgagcgttt cgccggtagc ccgcagatcg gccagcacca ccatactacc
     5941 ttgctgcaac gcctcctgta tggccgggag gctgtctttg atgacttttg ctttcccttc
     6001 ttcgtccacc cataccagga cattttttgc gggcccctgt ggagttgcga caaggcaggg
     6061 cagagggatc tcgccttttt tgcggacaag gcattttttg aattggacgg ggccggagga
     6121 ctcgcccatc cactctacat ttatgggatc gctgaacgcg ttggcatgca aatgcaggag
     6181 gtgccggatg atcgtcctgt tatccgcacg gccggcgaag gtctccctgt cggctttgta
     6241 gcggtccgcc agcgacagat cgatatcctt taatgtgcgt tcgttgggaa aagccgtatt
     6301 cacctctccg gtggaggtac agaataaggt ggagtcgggt aatatggcga tatcgccttc
     6361 ttttactgct gtgctgtcct tacaaagcca ttttctgaac caggtgacgg ccgcttcccg
     6421 tttgggtttg gcgatactgt gtccgtcgtc atagacgaac agcttgtatc gatcaggatg
     6481 gcccagcgcc gaatagacct gttgcaattc tttcgatgcg atctctacgc ctacctcatc
     6541 gacaaaatcg aatctcgctg ccagaaccag caggggtttt ggagcggcca tgaccaggaa
     6601 atcgtcgatc tcaagccgcg cctcgccttc accggggatg tgttgacagc cgtcgctggc
     6661 gccgatgagt ggcagattga agttgcgttc cctcgtggct acatagctgc agggcgccga
     6721 gacttttatc cgggggtcga agcctgcgaa ataggtggtt tgtgtaccgc cgccggaggt
     6781 gccgagacaa ccgatgcgtg aggtgtccac atcgccgcgg ctgcacagat agtccagccc
     6841 gcggacatta tcccatagtt cgtatgcggt cgttcctgtc ccgagcaacg tggcgccggc
     6901 gttgaaaagt gtgtgttcgg tcgtgccgag cgcgatgatg ggatgacctt tttcgtcagt
     6961 cagctgatac cgctcgccct gcgagatcgg gtccgcgacg agcacgacga agccatgagt
     7021 cgcaaacagg attgccgtac gctgatagga ggcatcttct ttcccattca ggctatggcc
     7081 gcagaatagc aggacgccgg gaaacggtcc gtgtccctgt gggatgtaga cattggccgt
     7141 gacatgatga ccgggcatgc tttgatagac aaccttctcg atccggtagc ccgtttcgga
     7201 gagggtgccg gtgatctgcg gatataatgg tgtgcgcgcc ggcaatgtgc cgagtatttc
     7261 caaatagcgt tttctgcagt tgtcccggta tgtgcgcatc gcggaggggc tggtcagcgc
     7321 ctttcggaga tcggttcgcc gcgcctccca ttggttgaac gctgcttgtt ccagataggt
     7381 atgcaccgta ttaaaaggcc accatgccga ctcgaagacg ttgtactgcc gttgcgcatt
     7441 ggagatcggg cagatgaaca aaatgatcaa agaggtaagc gccagtttcg ggatggctcc
     7501 caaccgcagt gtccccatcg aaaggtgttt ggtatacata ttaagatagt ttccggcggc
     7561 gatcgccggc catcggagta aaagtaaaaa aaaaatctgt attgtactat actataccaa
     7621 tatttttttt tataccttta actcgttatc gggaatgcca tatgattttc gaatccaaaa
     7681 caatctgatt atgcagcaaa agttctttct agcgttattg cttacggtgt gctatgcttt
     7741 cgcatcggcg cagagcccgc aggttaccgg gcatgtcgcc aacaaaaatt cttcggaggc
     7801 attgtcggcc gtcaccgtct ccgtaaaagg cacgaatatc cacacgctta ccgacagcgc
//
LOCUS       DASYSV010000582         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1525444, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000582 DASYSV010000000
VERSION     DASYSV010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525444"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1469
                     /gene="radA"
                     /locus_tag="VGQ51_18150"
     CDS             96..1469
                     /gene="radA"
                     /locus_tag="VGQ51_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV9038567.1"
                     /translation="MSKMRTAFFCTNCGHESAKWLGKCPSCEQWNTFVEEVITKDNKL
                     PETDWKKISNGKKETRSISLGEVVSDGEERIMSTDPELNRVLGGGIVPGSIVLVAGEP
                     GIGKSTLFLQNGLRLPGLRTLYISGEESEQQIKMRADRLGIRNDDFFLLTETSTQTIF
                     QEIKKLRPGLVIVDSIQTLQSPYIESSAGSVSQIRECTSEFQRFAKETHTPVFLIGHI
                     TKDGSIAGPKMLEHMVDTVLQFEGDRHYAYRILRTLKNRFGSTAELGIYEMTEQGMRG
                     VTNPSELLITQKEEQLSGIAIAATIEGLRPLLIEVQALVTQSVYGTPQRTVSGFDLRR
                     LQLLLAVLEKRGGFHFGVKDVFLNIAGGLKVEDPSIDLAVLCALLSSYEDSPLTSQTC
                     FAGEVGLSGEIRAVNRIDQRIAEAEKLGFERIFVSKYNQKGLGAGKFDIEVVPVGRVE
                     EVYQALF"
     gene            1497..2897
                     /locus_tag="VGQ51_18155"
     CDS             1497..2897
                     /locus_tag="VGQ51_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEV9038568.1"
                     /translation="MLRYPLLLLLSVALLISACRKNSFITGPNALVEFSTDTLLFDTV
                     FVSTGSITGSVRIINDNDQRLHLSSIRLMGGAQSTYHINIDGSPGPVATNIDLDAGDS
                     IYIFVAVQIDPGSANLPFVVQDSIQVDFNGNNRYIQLEAWGQNAHFLRNEVLTANTVW
                     DNTLPYVILGGLQVDTNVSLTIPAGCRVYFHADAPLLVDGTLLVNGQPYDSTRVSFRG
                     DRLDPPYNDFPGSWPGLYFRGTSTGNHLQYADIRNAYQAVVVEAPPAGPIPKVLLEQC
                     IIDNSYNAGIIGLQGSLQANNCLISNCGSNIELGGGGYYQFVQCTAVAYTNNYISHTQ
                     PVLSIADVIQQGSSVLTGGLQAGFTNCIFWGANGSVDDEVVVSRQGNSPFSVNFADCL
                     WKIKNAPSGVTATGIIANQDPQFDSVNNFRPYYDFHLRAGSPAIGAGTSTTLGIDLDG
                     NPRPALKPDLGCYQRQ"
     gene            3027..>3348
                     /locus_tag="VGQ51_18160"
     CDS             3027..>3348
                     /locus_tag="VGQ51_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV9038569.1"
                     /translation="MLRVLLFSALMLVGANSVYDFKVTGLDGSPLNISQYKGKKILIV
                     NTASKCGYTPQYADLEKLYETYKGKLVVIGFPANNFGQQEPGTNKEITEFCTKNYGVT
                     FPMSE"
BASE COUNT          833 a          863 c          856 g          796 t
ORIGIN      
        1 cttcttttca tttttcacct gcagtttttc atttcaacta atcggatttc caaaataggg
       61 accttgaaga gtacacagga tgttaacttt accgcatgag taagatgaga acggcatttt
      121 tctgtaccaa ctgcgggcat gaaagcgcaa aatggttggg caaatgtcct tcctgcgaac
      181 aatggaacac ttttgtcgaa gaagtcatca ccaaggacaa caaattgccc gagactgatt
      241 ggaaaaaaat ttccaacggc aaaaaagaaa cacgttccat atcgctcgga gaggtagtaa
      301 gcgacggaga agagaggatc atgagcacgg accctgagct taacagggta ttgggtggcg
      361 ggatcgtccc aggtagcatc gtgctggtag caggtgaacc tggcatcggc aaaagcacgc
      421 tcttcttaca aaacggtctg agactgcccg gtctgcgaac tctctatatc agcggagaag
      481 aaagcgaaca gcaaataaaa atgcgcgccg atcgtctcgg tatccgcaat gatgatttct
      541 tcctgctgac ggagacttcc acgcaaacga tcttccagga gataaagaag ctgcgccccg
      601 ggctggtgat cgtagattcg atacagaccc tgcagtctcc ttacattgaa tcttcggccg
      661 gcagcgtttc ccagatccgg gaatgtacct cggagttcca gcgttttgca aaagagactc
      721 atactcccgt cttcctgata gggcatatta ccaaggacgg gagcatcgcg ggtccgaaga
      781 tgctggagca tatggtagac accgttctgc aattcgaagg agaccgtcat tatgcatatc
      841 gcatccttcg cacgttgaaa aatcgttttg gcagcacagc ggaactcggc atttatgaaa
      901 tgaccgagca gggcatgcgc ggcgtcacca atccgagcga gttgctgatc acgcaaaaag
      961 aagaacagct cagcgggatc gccatcgcgg cgaccatcga aggtctccgt cctttactga
     1021 tagaggtgca ggcgctggtt acgcagtcgg tatatgggac gccccagcga accgtcagcg
     1081 gtttcgatct gcgaagattg caattgctgc tggcggtgct tgaaaaaaga ggaggtttcc
     1141 atttcggcgt aaaggatgtt tttctcaata tcgcgggcgg cctgaaagtg gaggatcctt
     1201 cgatagacct ggctgttctc tgcgcattgc tcagcagcta tgaagattcg ccgttgacgt
     1261 cgcagacctg ttttgccggt gaggtagggc tcagcgggga gatccgcgcg gtcaatcgca
     1321 tcgatcaacg catagccgaa gccgaaaagc tcggattcga acgcatcttc gtttccaaat
     1381 acaaccaaaa gggattgggt gccggaaaat tcgatatcga ggtcgtgccg gtcggtcgcg
     1441 tcgaggaagt gtaccaggcg ttgttctgac gacaaacata ttgtatcttc gattcgatgt
     1501 tgagatatcc gctcctgtta ttgttgtcgg tcgccctgct gatatccgcc tgccggaaga
     1561 attctttcat caccggtccc aacgctctcg tcgaattttc taccgacacg ctccttttcg
     1621 atactgtctt tgtctccaca ggctcgatca ccggatcggt aaggatcatc aatgacaatg
     1681 accaaaggct ccatctttct tccatccgtc tgatgggagg ggcgcaatcc acgtatcata
     1741 tcaacatcga cggatcgccg ggacccgtcg ccaccaatat cgatctcgat gcgggcgata
     1801 gtatttacat tttcgtggcg gtgcagatcg atcccggatc ggccaatctg cctttcgtag
     1861 tgcaggacag cattcaggtg gatttcaacg gcaataaccg gtacatacag ttggaagcct
     1921 ggggacaaaa tgcgcatttc ctgcgcaacg aggtgctaac tgccaatacg gtctgggaca
     1981 acacgcttcc ttatgtcatt ctcggaggtc tgcaggtgga caccaatgtc tcgctgacca
     2041 tccctgccgg ctgcagggtc tatttccatg ccgacgcccc attgcttgta gacggcacac
     2101 tactggtcaa cggtcagcca tacgacagca cgcgcgtgtc tttcaggggg gacaggctcg
     2161 atccgcctta caacgatttc ccgggaagct ggccgggact ttatttccgc gggaccagta
     2221 ccggcaacca tcttcagtat gccgatatcc ggaatgctta ccaggcggtg gtagtcgagg
     2281 ctcctcctgc gggcccgatc cctaaggttt tgttggagca atgtatcatc gataacagct
     2341 ataacgcggg catcatagga ttgcaaggga gcctccaggc caacaattgc ctgatcagta
     2401 attgtggcag caatatcgaa ctgggcggtg gcgggtatta tcaattcgtc caatgtacgg
     2461 cggttgcata taccaacaat tacatctccc atacccaacc cgttctatcc attgcggatg
     2521 tcatacagca gggaagctcg gtactgacgg gggggcttca ggccggattt accaattgca
     2581 ttttctgggg cgccaacggc agcgtggatg atgaagtagt ggtaagcaga cagggcaaca
     2641 gtccctttag tgtcaatttc gccgattgcc tatggaaaat taagaatgcc ccttccggcg
     2701 tgacagctac gggcatcatc gccaatcagg atccgcagtt cgacagtgta aataatttca
     2761 ggccctatta tgatttccat ctcagggccg ggagccctgc catcggagcg ggaacatcaa
     2821 cgaccctcgg catcgatctg gacgggaatc cgcggccggc tttgaaaccc gatctgggct
     2881 gttatcagcg gcaataagac cgccggcgcc gcctaccctt tttttaaacc atctatcctt
     2941 agaaatcttc actactttaa actttagcgt tcgtactctg tactattttt aggaacttag
     3001 ataccttcaa ccaaaatcat tctactatgc tacgcgtact tcttttttca gcactgatgc
     3061 tcgtcggagc caatagcgtt tacgacttca aagtgaccgg gctggatggg agcccgctca
     3121 acatctccca atacaagggc aagaagatat tgatcgtcaa cactgccagc aaatgcggtt
     3181 atactcctca atacgccgat ctggaaaaac tgtacgagac ctataagggc aagctcgtcg
     3241 tcatcggttt ccctgcaaat aatttcgggc aacaggaacc gggtaccaac aaagagatca
     3301 ctgaattctg cacaaagaat tatggtgtca ccttcccgat gtctgaaa
//
LOCUS       DASYSV010000583         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1780888, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000583 DASYSV010000000
VERSION     DASYSV010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780888"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VGQ51_18165"
     CDS             complement(<1..642)
                     /locus_tag="VGQ51_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038570.1"
                     /translation="MSYVSLENSESFLKCFEVIAEDKSCIVSESVLYFSGKTGKGFMT
                     VAGLATGIEVLAVNVSALAEDLVFNFLPSTDAFSYLLSFDEIEIPGNGDSDLTLNSSA
                     VPALFSSVSIADAASGRVVNYPKDRVVRSILVRVKKSSLDHLIQDFDAGTIGRILGTA
                     GMGETLTWPITFKYRLLLNDFMTEIVQHPFRELFTIRNVSVLIEYLLQQFFVMD"
     gene            904..1434
                     /locus_tag="VGQ51_18170"
     CDS             904..1434
                     /locus_tag="VGQ51_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038571.1"
                     /translation="MNNSSKKSGCNSETRSMLKNQHKVVYSICRFFAHNYQEHQRLFT
                     NIIAAASQNIRSRKKESSEDKQTLLLRACINMAALHSITMEMEPPTVRTIQFKSSDYH
                     QCMTKFRESMGEISDLEKFRLFMHLEKVAPGDMAGLTGLDAINPGKYYPGRKNETSTT
                     QGKNLIPYLKATLIWS"
     gene            1425..2054
                     /locus_tag="VGQ51_18175"
     CDS             1425..2054
                     /locus_tag="VGQ51_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038572.1"
                     /translation="MELKKLLQQWDSFPEVSMEERPVLSSDLEKIVVKNPLSDAFYLR
                     RKLLLRIAVIALLWLFNSWQLRGQWRTSGDDLYVTGALFLLLSFSLYFHIRLILFADY
                     PTLLALPLIDFLSKLETVLDKYIVSFKLVSAMAGFYILKAIEFLLHQFSKDAYGTLSR
                     NDWYKWLILIFLAVSVDILLLHTLIPRYRKLQATVGRYRDGILTKDPRK"
     gene            2127..2756
                     /locus_tag="VGQ51_18180"
     CDS             2127..2756
                     /locus_tag="VGQ51_18180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9038573.1"
                     /translation="MQNILIADDHSIVRAGLKLLINNCIHDVIIDEAEDGEAVIKKMK
                     GAHFDLLVLDINMPHTESFSLTAYLLKEYPGLKIIIFTMNQEVFFAKRFLKIGAHGYL
                     NKYSKESEIKYAIQKVLSGKRYISDNLAEILSGELTKKLKDNPFEDLSDREFEVVLQM
                     LKGSSVSEIANTLSLHKSTVGTHKTRILDKLKISNTVELINLARLYEII"
     gene            complement(2749..3555)
                     /locus_tag="VGQ51_18185"
     CDS             complement(2749..3555)
                     /locus_tag="VGQ51_18185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV9038574.1"
                     /translation="MYKAVFLDMDGTLLRSDHSVSEETIRKIRALTAQGVTVVLVSAR
                     PVDAVFPTFRNIGLPEQTPLIALNGSYIVQSGEPILDVRIDLGMTARVTREVRPYKAT
                     IAYYLQREWYSEASDAWTDHEQKIMDVQLRVDSIERLIEEWGGKGKAPNKMMVMSEPS
                     NINGIQEHLRSLFNGQLNVYPSKATYLEVMDSRGSKAGAVRFIGERMGLVPAEIIAMG
                     DNYNDIGMLEFAGLGVAMGNAPDDIKAVADDVTDTNNNDGVRKALEKYFK"
     gene            complement(3580..>4250)
                     /locus_tag="VGQ51_18190"
     CDS             complement(3580..>4250)
                     /locus_tag="VGQ51_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEV9038575.1"
                     /translation="GYWAILMVAGVPGADPLSMTGNAGYRLDVFVLGENHMYHGEGIA
                     FDPEGLLSTLPAVVNVVAGYFAGVFISRKGKEYEGLTRLLLWGCGLVVLALAWNTVFP
                     VNKKLWSSSFVLLTIGIDLVLLAFLIYVIEFRHRVAWTPFFNVFGKNPLFIYLLSELL
                     VVILFTIPAGSNGESVYEWVNRVFYQALLPGAIGSLFFALTYMIVCWSVGKWLDHRKI
                     YIRV"
BASE COUNT         1112 a         1195 c          961 g          982 t
ORIGIN      
        1 gtccataacg aagaattgct gaagaaggta ttcgatcagc accgaaacat tccggatggt
       61 gaacaattcc cggaaaggat gttgtacgat ctccgtcata aagtcattga gcagcagccg
      121 gtatttgaag gtgatcggcc aggtcagcgt ctcacccatg cctgcagttc cgaggatacg
      181 gccgatggta ccggcgtcaa aatcctggat caggtgatcc agcgaagact ttttcacccg
      241 gaccagtatc gaccggacca cacggtcctt agggtagttg acgaccctgc ccgacgccgc
      301 atcggcaatg gatacggagg aaaatagcgc cggaacagct gaggaattga gagtcagatc
      361 actatccccg ttgccgggga tttctatctc gtcgaaacta aggagataag agaaagcatc
      421 tgtagagggt aagaaattga ataccaagtc ttccgcgagg gccgatacat tcaccgccag
      481 tacctcgatg ccggtcgcca acccggcgac tgtcataaaa cctttgcccg ttttcccgga
      541 aaaataaagt acggattccg atacgataca tgatttgtct tctgcgatca cctcaaaaca
      601 tttcagaaaa gattctgaat tttcaagaga tacatagctc attgcgttaa gtttatttgc
      661 ccgtcaaaag cacttctccg ctaccaatcc gcgacaaggt attaattagg ctcccttgga
      721 aatatagtaa aaggttgaat tgatcgtagt tttttaacga catcacccct gcgataaaca
      781 cttaaccata ccattttgcg gtccttttac gttattaaaa tgaacggcgc agttatttcg
      841 aaaacgaatt tcgatacata tttaccgcct gatcccgacc catgtataaa atccaaaccc
      901 actatgaaca attcctccaa aaagagcgga tgtaattccg agacccgctc gatgctgaaa
      961 aatcagcaca aggtggtcta ttcgatctgc cggttcttcg cccacaatta ccaggaacac
     1021 cagcgtcttt ttaccaacat cattgcggct gcctcgcaga atatccggtc caggaaaaaa
     1081 gagagcagtg aggacaaaca gacccttctg cttcgcgcct gtatcaatat ggccgcgctg
     1141 cattccatca ctatggaaat ggagccccct accgtcagga ccatccaatt caagtcttcg
     1201 gactaccatc agtgcatgac gaaattccgc gagtcgatgg gtgagatctc cgatctcgag
     1261 aaatttcggc tattcatgca tctggagaag gtggcccccg gcgatatggc cggcctgacc
     1321 ggtctcgacg ccatcaatcc gggcaagtac tatcccggtc gcaaaaacga aacgtcaacg
     1381 acccagggca agaacctgat cccttattta aaggcaacgt tgatatggag ctgaaaaagc
     1441 tgttacaaca atgggacagt tttcccgagg tctccatgga agagcggccc gtcctgtctt
     1501 ccgacctgga aaagatcgtc gtgaaaaacc cgttgtcaga cgccttctat cttcggagaa
     1561 aactgctcct gcgcatcgcc gtcatagccc tgctctggct gttcaactcc tggcagcttc
     1621 gcggacaatg gagaaccagt ggtgacgacc tctacgtcac cggagcgctg ttcctcctgc
     1681 tgagtttttc tctctatttt catatccggc tcatcctttt cgccgactac ccgacgctgt
     1741 tggccctccc gctgatcgat ttcctgagca agctggaaac cgtcctggac aagtatatcg
     1801 tctcctttaa gctcgtcagc gccatggcgg gtttctatat tttaaaggca atagagttcc
     1861 ttttgcacca gttcagcaag gacgcctatg gcacactcag ccgaaatgac tggtacaaat
     1921 ggctgatcct tatctttctt gccgtttcag tcgacattct tctgcttcat accctcattc
     1981 cccggtaccg caagctgcag gctacggtcg ggcgctaccg ggacggcatc ctgacaaaag
     2041 atccccgtaa ataaacaggc ggaacaaaat ttcctatata attattatcc tctatatttg
     2101 ggataagttc ccctcaatcc aagcatatgc agaatatctt aatcgctgat gaccattcca
     2161 tcgtcagagc cggattaaaa ttactgatca ataattgcat ccacgatgtc atcatcgacg
     2221 aggcggagga tggagaggcc gtgatcaaaa agatgaaagg ggctcacttc gatctgctgg
     2281 tcctggatat caatatgccg cacaccgaat ccttcagtct cacggcctat ctgctcaagg
     2341 aatacccagg tctgaagatc attatcttta ccatgaacca ggaagtcttt ttcgccaaac
     2401 ggttcctgaa gatcggcgca catggctacc tgaacaaata ttccaaggaa tcggagatca
     2461 aatatgccat ccagaaggta ttgagcggga aacgctatat cagcgacaac ctggccgaga
     2521 tcctgtcggg cgaactcacc aaaaagctaa aggacaaccc ctttgaagat ctttcggatc
     2581 gcgagttcga ggtcgttttg caaatgctca aaggaagttc ggtatcggag atcgccaata
     2641 ccctcagcct gcacaagtcc acggtaggga cccataagac aaggatcctc gacaagctga
     2701 agatatccaa taccgtagag ctgatcaatc tcgcgcgtct ctacgagatc atttgaagta
     2761 tttttccagc gccttcctca ccccatcatt attattggtg tctgtgacat catcggcgac
     2821 agctttgata tcatcgggag cattgcccat cgccacgccg agccccgcaa actcgagcat
     2881 tccgatgtca ttataattat cgcccatagc gatgatctcc gccggcacca gccccatccg
     2941 ctcgccgatg aaccggacgg cacccgcctt ggaaccgcgg ctgtccatca cctcgagata
     3001 ggtggccttg gaaggataaa cgttcagctg accgttgaac aaggaccgca gatgttcctg
     3061 gattccgttt atattggatg gctcgctcat gaccatcatc ttgttcggcg ctttcccctt
     3121 gccgccccac tcttcgatca gcctttcgat gctgtcgacc ctcagctgca catccatgat
     3181 cttctgctcg tgatcggtcc atgcatcgct agcctccgaa taccattccc gctgaagata
     3241 ataagcgatg gttgccttat atggtctcac ctcgcgggtc actctggccg tcatcccgag
     3301 atcgatccgc acgtcaagta tgggctcgcc ggattgcacg atatagctgc cattcaatgc
     3361 gatcagggga gtctgttcgg gcaggccgat attccggaaa gtaggaaata cggcatcgac
     3421 aggccgcgca gagaccagga ccactgtcac accttgcgca gtaagtgccc ttatctttct
     3481 gatcgtttct tcgctgacgg aatgatcgct tctgagcaac gtaccgtcca tatccaggaa
     3541 taccgctttg tacatgcggc gcaagataca ctattgcacc tacactcgga tatagatttt
     3601 cctatgatcc agccacttcc cgacgctcca gcacactatc atataggtca gtgcgaaaaa
     3661 caacgacccg atggcgcccg gcagcaaagc ctggtaaaac acccggttga cccattcgta
     3721 gacactttct ccattgctgc ctgcggggat ggtgaaaagg atgaccacca acagctcgga
     3781 taacagataa atgaacaatg gatttttccc gaatacattg aaaaaaggcg tccacgcgac
     3841 cctatgccgg aactcaatga catagatcag aaaagccagc aacaccaggt cgatgccgat
     3901 ggtcaggagg acgaacgaac tgctccaaag ttttttgttg acagggaaga ccgtattcca
     3961 ggccagcgcc aacaccacaa gaccgcaacc ccaaagcaac agacgtgtca gcccttcata
     4021 ttcttttcct ttccggctga taaagacgcc ggcgaaatac cccgccacca cattgaccac
     4081 ggccggcaaa gtgctcagca acccctccgg atcgaaggcg atcccttccc catgatacat
     4141 gtggttctcc cccagcacga atacatcgag acgatagccc gcgttccccg tcatactcag
     4201 cggatcggcg cccggtacac ccgccaccat caggatggcc caataaccga
//
LOCUS       DASYSV010000584        12358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1015367, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000584 DASYSV010000000
VERSION     DASYSV010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12358
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015367"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VGQ51_18195"
     CDS             <1..1134
                     /locus_tag="VGQ51_18195"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEV9038576.1"
                     /translation="SQNKKTPDASTQIQYAGSQSCARCHADIYEQHLQSFHHLTTVAA
                     TRQTLKGDFDSANKFYLNDDLFIAAEKKQDSFFQTAYVSGVPRLSRPFDIIVGSGKRG
                     QTSIYWFQNYLFELPLTWFTETNEWTISPGYSRKAGFNRPITSRCLECHSTYFQDITN
                     KDSKADEFSKTNYILGVECEKCHGPGAEHIAFHDKNPEEKTGHAIFNPAKGTRQQSLD
                     LCRYCHGGRLSKSKPSFTFQPGDKLFDFFREDTGRPVSDIDVHGNQYGMLAASKCFRN
                     STMTCLTCHDAHKNQSAQAAQFYVSCETCHSSSSHTDCKLASSVSRDVLRTNCISCHM
                     PEEASKAIMVIRNGQSIPTSAHMRSHYIAVYKNISNQILSARK"
     gene            1142..4438
                     /locus_tag="VGQ51_18200"
     CDS             1142..4438
                     /locus_tag="VGQ51_18200"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEV9038577.1"
                     /translation="MRKVCLFSLGLLTILGFFSCKPKTLFETVSASHSNIHFTNTLEK
                     HKAFGILYFLYYYNGGGVSIGDINNDGLPDIYFTANHKGGNKLFLNKGNFEFEDITEK
                     AGVAGTSDWCTGSTMADVNGDGLLDIYVCAVQGEHGLKGHNELFINNGNGTFTESSKK
                     YGLDLAAFSGQAVFFDYDHDGDLDCFILNQSHHPNENLSDTSRRRKFDPNAGGRLYRN
                     DLSTTGKFTDVSAQAGIYQSDLGYGLGVSVADLNNDGFDDIYIGNDFHENDYYYINNG
                     DGTFTESGAKHFGHYSRFSMGNDIADYNNDGEPDIITVDMLPPDEKTVKTYGSEENPE
                     IYKYKIKGHGFQDQVSRNCLQTNNGSGQSFSDVALMDGVSATDWSWCPLFADFDNDGK
                     KDLFISSGIVKRPTDLDYVRFVSDLAAHVNRNSSDEFDEQTIAKMPDGSSHPFLFKGN
                     LDKGFTDVSGDWGTRKMKGYFNGAAYADLDNDGNLDLVINCIDQPAVVLKNTSQPKDF
                     LNIAFKGTGMNKFGIGAKAWLFSHNTSHKPVTQYEELMLTRGFQSSSDTRLHFGLDSL
                     TVDSLLIVWPDQKYQLIKNPAINKIDTCDQTGAAGHFNYPEHFPPRPPQLENITANIK
                     CDWKHIENNFNDFAVQMLLPHLESTRGPKVAVGDINNDGLSDFYVCGARGHAGALMMQ
                     EPDGNFKPLDTAEFAKFKFCEDVDAVFFDANGDGFQDLLVISGGNEPSINSPLFLQDR
                     LFINDGHGHFKASPDFMVQQLYNKSCVAVADVDHDGDMDVFIGTLSRTEGTAFGTPMK
                     SYLMLNNGHGKFDITSDSVMPLTRLGTVTSARFADVNNDGWPDLIVTGEWMPVKVFIN
                     HHGKFSEHDIPNSTGLWQTVLATDVNGDGHPDLLVGNWGHNSKLWAGKNGPLKLYVKD
                     FDGNGTVEQLMCYTIDGKEYTFLPKDELEREIPVLKKYYLTYSEVAGKDVQYMFYDLF
                     RNYIELKAETLSTSCFINDGKGNYVRTDLPDELQKAPVFSFAQLPDSSYLAGGNFYGV
                     RPYEGKYDALCPTVFSFDKHSGRASARDILPDVNGEVRDLKWLHTAKYGDILVAARNN
                     DSLLFFKYKK"
     gene            4445..6124
                     /locus_tag="VGQ51_18205"
     CDS             4445..6124
                     /locus_tag="VGQ51_18205"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEV9038578.1"
                     /translation="MTRRFGLVMILAALAAGCSKHSDSSNNVVTPPAGPFTLNSTTLN
                     GIATGSPFYNINFSPVIHCSFSGPVDHSSLTNGFYFKDNTGTSVAWSVTYQNGDSVLV
                     IQPSSALQPLTKYDFGFNTSLQSQHKINLQSASDVPFMTAIDSTDKFPRITDSALLDL
                     VQRQTFTYFWDNGNAVSGLAQERTTSGSTVTTGGSGFGVMAILVAIQRNFITRAQGLA
                     RIATIVNFLSDSTKVTRYHGAFSHWMNGSTGATIPFSANDDGGDIVETSYMMEGLLCA
                     RQYFNSGTDPTEMGLRAKINALYGGVDWNWYRQNGLNVLYWNWSPDHAWAVNVPVNGW
                     DEALITYVLAASASPDSIPRIVYDNGWARNGAIQNKKSFYGITLPLGPDYGGPLFFAH
                     YSFLGIDPRGLSDTYANYWAQDTAHSRINYLYCVNNPKNYFGYSHLCWGLTASDEQNG
                     YSAHSPTNDDGVISPTAAISSMPYTPTESMNALRFFYYTLGDRLWKQYGFIDAFDPAT
                     GWYDTDFLAIDQGPEIVMIENYRSGLLWNLFMSCPEIKAGMKRLGFSGPSL"
     gene            6134..7456
                     /locus_tag="VGQ51_18210"
     CDS             6134..7456
                     /locus_tag="VGQ51_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEV9038579.1"
                     /translation="MKIPAIIFALASTLSAMAQPGLSDSALLDIVEKQTFEYFWTGAE
                     PNSGMAPERIHMDNVYPDHDQGIIATGGSGFGVMAILAGIHRHFITRGQGYARLEKIV
                     SFLEKADRFHGAWPHWLNDTTGHVKPFGRDDNGGDLVESCYLLQGLLCVRQYFKDGNP
                     EEKKLAARIDELWRGVDFDWYRNGRQNVLFWHWSPTYGWKMNFPVHGYNECLILYVMA
                     ACSPTHTIPAEVYNEGWAQNGAIHQVSFYKKDTLHLHMQGNPPHGGPLFWSQYSYLGL
                     DPHGLKDRYADYWKENSTQSIINYTWCVDDPKHYAGYGPDNWGLTASYSVAGYAAHAP
                     DLKNDLGVISPTAAISSIPYTPRQSMAAIRHWYKMPGGKLLGKYGFYDAFSERAAWYP
                     PRYLAIDQGPEVAMIENYRSGLLWRLFMSCPEVHAGLKKLGFESPWIK"
     gene            7462..8850
                     /locus_tag="VGQ51_18215"
     CDS             7462..8850
                     /locus_tag="VGQ51_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEV9038580.1"
                     /translation="MRNGFIFLALLSTQSVLPQATIRPVGIIRGLSDSALLDVVQRQT
                     FRYFWDFAHPVSGLARERDNTVRAEYYWDYINEADGEPNFSKDTFGPEACAIGGTGFG
                     ILSTIVAVNRKWIGRDTALRRLVKIVDFLTHADCYHGIYPHFMNGATGRTIPFGRLDD
                     GADIVETSYLMMGLLTAREYFNGHNPLETYFRNRVTAMWADADWNWHSNNGSKKLYWH
                     WSPRNDFNMNFPVFGWDEALITYVMAASSQTHPIPKELYESCWVGSASWRNGKEYYGI
                     KLPLGNFEWGGPLFFEQYTFMGVDPNGLKDDHGIDYAEQTRNHTLINRAYCIDNPKKF
                     KGYGADCWGLTAGDSYKGYVAHCPQDDRGVIQPTAALSSFPYTPEYSMQALKHFYYDL
                     GDKIWGPYGFADGFSESHNWYAKTHLAIDEGPIVVMIENYRSGLIWNLFMRIPDVQAG
                     LKKLGFQSPDMK"
     gene            8847..11315
                     /locus_tag="VGQ51_18220"
     CDS             8847..11315
                     /locus_tag="VGQ51_18220"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEV9038581.1"
                     /translation="MNGQYIKYALIGAACLILAGTSYAQVNSVGKIVSYTKIPGGITG
                     RLAGAIFEVRAWSPNIIRVRVSRRKSFDDFSYALIQPDPSTQVKVGIVEKDDRILLST
                     TSLHAEIEKTPDFRVTFRNNAGAVINEDMMGDAFGTSFIGNKVTIYKKLQDGERFTGL
                     GESLGPLDRRGMGITLSNTDNYKYGDPRLPMYSSIPFYIGIHHDQVYGLFFHNTYKSF
                     FNFGLSTPDFSSISAEGGDADYFLLYDSTIGGILKAYTSLTGRMPLPPLWSIGYHQSR
                     CTYYPQDKALWIAQTFRRKQIPIDCIVLDADYLHEYEPFRIDKRRFPDMRGLAGQLQK
                     MGIELTASVNPGIKLDTTYPANADGLKQDVFIKYTDGRPFVSEIAPSLNHFVDFTDPK
                     ARAWWIDNMKFLPQNGIYGYWNDMNEPAVGGSYLPDNLVFDFDGRKASAAEAKNVYGM
                     EMARSSYESGLNYNDGRRPFVLTRSAFAGIQRYSAVWSGDNQAKDEYLLGGVLLNCQL
                     GLSGVPFVGPDLGGYIGDGNKQLYKRWIEVGIFSPFVRNHREFFGAANEPWAYGEEGE
                     AISKTYIGFRYRLMPYIYSLFYETAQTGMPVARSLCIDDPFDPKVYDPAYQYEFLFGD
                     ALLVTPMTSTETYKRIWLPRGDWYDLWSDSLLTGNKEVGGEYPIYRLPLFVKASSILP
                     MQSLIQSTKQRPGDTLQVHIFYGKEKGRFVYYEDDGATFAYRQGHYCQRTIGFDPVEQ
                     RIVFGTQEGDYSSCFGHIEVILHGFPPVTGSRPDTCRILDELGEMEDLYDPATFRQLR
                     AGLPVIRTQTITLANSKEQLIIKW"
     gene            11320..12147
                     /locus_tag="VGQ51_18225"
     CDS             11320..12147
                     /locus_tag="VGQ51_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEV9038582.1"
                     /translation="MKSFQLTQQQLADYNRDGYLIVRNFLTSEEIAKLYAIATGDATL
                     SKHAFDLNDLTGKKTKLTLWYNPGDDAYGLLTRSKKMVDSANQLMDGKSPVCHFHSKL
                     MQKEPKVGGAWEWHQDYGYWYKNEFLFPDEMVSVMIAITKATKENGCLQVIGGTHKMG
                     RIEHGFSGEQVGASQHYVDLALKTMPLVYVELEPGDALFFHSNTLHRSEANLSDRPRW
                     SLISVYNRQENIGYNEPNGSHTTAVNVVPDEALLEWDTKGVGESSGFLQKEKDEALK"
     gene            12148..>12358
                     /locus_tag="VGQ51_18230"
     CDS             12148..>12358
                     /locus_tag="VGQ51_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylosidase"
                     /protein_id="HEV9038583.1"
                     /translation="MKTLLYMSIFLAAVRGQAQEKTYCNPINLDYGYTPIPDFTQWGR
                     HRATADPAMVNYKGDFYLFSTNQWGY"
BASE COUNT         3140 a         3307 c         3096 g         2815 t
ORIGIN      
        1 tctcagaata aaaagacgcc tgacgcctcc acacaaattc aatatgcagg ctcgcaatcc
       61 tgcgctcgtt gccacgcaga tatctatgag caacatctgc aatccttcca tcatctgacc
      121 accgttgcgg caacgaggca aaccttaaaa ggcgacttcg acagcgctaa taaattctat
      181 ctcaacgacg acctttttat tgccgcagaa aaaaagcagg atagcttctt tcagaccgcc
      241 tatgtatccg gcgtcccgag actctccagg ccgttcgata tcatcgtggg atcgggcaaa
      301 cgcggccaga caagcatcta ctggttccaa aattatttgt ttgaattacc actgacctgg
      361 ttcaccgaaa ccaacgaatg gacgatcagc cccgggtatt cccggaaagc gggttttaac
      421 agaccgatca cctcgcgctg tcttgaatgc cattccacat attttcagga catcacaaat
      481 aaggattcca aagcagatga gttttccaaa accaattata tccttggagt cgaatgcgaa
      541 aaatgtcatg gtccgggggc agagcacatc gccttccacg acaagaatcc cgaagagaag
      601 accggccacg ccatttttaa ccccgcgaag ggaacccgcc agcaaagcct cgatctgtgc
      661 aggtattgcc atggcggcag actttcaaaa tccaaacctt cattcacgtt tcagccgggc
      721 gataagctct tcgacttctt tcgggaggat acgggaaggc ctgtttcgga catcgatgta
      781 cacggcaatc aatatggcat gcttgcggcc agtaaatgct tcaggaatag cacgatgacc
      841 tgcctcacct gtcatgacgc gcataaaaac caaagcgcac aggcggcgca attctatgtt
      901 tcctgcgaaa catgtcacag cagttcctct cacaccgatt gtaaactggc gtcatccgtc
      961 agccgggatg tgttacgaac gaattgcata tcttgccata tgccggaaga agcgtcgaaa
     1021 gcgatcatgg tgatccgcaa tggccaatcg atcccgacat cggcacatat gcgttcgcac
     1081 tatatagccg tatataagaa tatttctaac caaattctaa gcgcacgaaa ataacaggtt
     1141 catgagaaag gtttgccttt tttcgcttgg gttattgaca atactgggtt ttttctcctg
     1201 taaaccaaag acgttgttcg aaactgtctc tgcttcgcat tccaatatcc atttcaccaa
     1261 tacactcgaa aagcacaaag ccttcgggat cttatatttc ctgtactact acaatggcgg
     1321 cggcgtatcc atcggcgaca tcaataacga cgggctgccc gacatctatt ttaccgccaa
     1381 tcataaaggc ggcaacaaac tctttttgaa taagggcaat ttcgaattcg aagacatcac
     1441 ggaaaaggcc ggagtggccg gaacgtccga ttggtgcacc ggctcgacga tggccgatgt
     1501 caacggcgac ggactgttgg atatctatgt ttgcgcggtc cagggcgagc acggcctgaa
     1561 gggccataat gaattattca tcaacaatgg taacggcacc tttaccgaaa gctccaaaaa
     1621 atacgggctg gacctggccg ccttctccgg acaggccgtt ttcttcgatt acgatcacga
     1681 tggcgacctg gattgtttta tattaaatca atcgcatcat ccgaacgaga atctgagcga
     1741 tacgagcagg cgaaggaagt tcgaccccaa tgccggcggc cgtttgtaca gaaacgatct
     1801 tagcactacc ggaaaattta ccgacgtatc ggcacaggca ggcatctatc aaagcgacct
     1861 gggatatggt ttgggtgttt cggttgcaga cctgaacaat gacggtttcg acgatatcta
     1921 tatcggtaac gattttcacg agaacgacta ttattatatc aataacgggg atggaacttt
     1981 taccgagagc ggagcaaaac attttggaca ttattccagg ttcagcatgg gtaacgatat
     2041 agccgattac aacaatgacg gggaacctga tatcatcacc gtagacatgc tgccccccga
     2101 tgaaaaaacc gtaaagactt atggcagcga agaaaatccg gagatctata aatataagat
     2161 caagggtcat ggcttccagg accaggtttc gaggaattgt ttgcaaacga ataatggcag
     2221 cggacaaagc ttcagcgatg ttgccctgat ggatggtgtc agcgcgacgg actggagctg
     2281 gtgcccctta tttgccgatt ttgacaacga tggaaaaaaa gatctgttca tttcgagcgg
     2341 gatcgtaaag cgaccgaccg atctggatta tgtgcggttc gtttcggacc tggcggcgca
     2401 tgtcaaccgc aattcgtcgg atgaattcga cgaacagacc attgccaaaa tgcccgacgg
     2461 ttcctcccat ccattccttt ttaaaggcaa cctcgacaaa ggatttacag acgtcagcgg
     2521 agactggggg acccgaaaga tgaaaggtta tttcaacgga gcggcttatg ccgatctgga
     2581 caatgacgga aatctcgacc tggtgatcaa ctgcatcgac cagccggccg tggttttaaa
     2641 gaatacatcg cagccaaaag attttttgaa tatcgcattc aaagggaccg gcatgaacaa
     2701 attcggtatc ggcgccaagg cgtggttgtt ttcacataac accagtcata agccggtgac
     2761 acaatacgaa gaattgatgc tgacccgcgg ttttcagtcc tcgtccgata cccggttaca
     2821 tttcggattg gacagcctga ctgtggatag cctgctgatc gtctggcccg accagaaata
     2881 ccagctcatc aaaaacccgg cgatcaataa gatcgatacc tgtgatcaaa ccggggcagc
     2941 cggtcatttc aactaccccg agcatttccc gccaaggccg ccgcaactgg agaatataac
     3001 cgcgaacata aaatgcgatt ggaaacatat cgagaacaat ttcaatgatt tcgccgtcca
     3061 aatgctcctg ccgcatttgg aaagcacgcg gggcccgaag gtggccgtcg gcgatatcaa
     3121 taacgatggc ctgagcgatt tttatgtttg cggcgcccgc ggccatgccg gcgccctgat
     3181 gatgcaggaa ccagatggca atttcaaacc cctcgatacg gccgaatttg cgaaattcaa
     3241 attttgcgaa gacgtagatg ccgtcttctt tgacgccaac ggcgacggct ttcaggatct
     3301 gcttgtcatc agcggaggca acgagccttc catcaactct cctttattct tacaggatcg
     3361 cttattcatc aacgacggcc atgggcattt caaggccagt cccgatttta tggtccagca
     3421 gctgtacaat aaatcatgtg ttgcagttgc cgatgtagac catgacggcg atatggacgt
     3481 ctttatcgga acgctttcca ggacagaggg taccgcattc ggaaccccca tgaaatcgta
     3541 tttaatgcta aataacgggc atggcaaatt cgatattaca tcggatagcg ttatgcccct
     3601 gacacgtctt ggaaccgtca ccagcgcccg gtttgcggat gtcaataacg acggatggcc
     3661 cgatctgatc gtcacgggag aatggatgcc cgtcaaggtc tttatcaacc atcatggaaa
     3721 attctcggag cacgatattc cgaactccac gggtctttgg cagaccgttt tggcgacgga
     3781 tgtgaatggc gacggtcatc ccgatctgtt ggtcggcaat tggggccaca actccaaact
     3841 atgggcggga aagaacggac cgttgaaatt atatgtcaaa gacttcgacg gcaacgggac
     3901 cgtcgaacaa ctgatgtgct ataccatcga cggaaaagaa tatacattcc tgccaaaaga
     3961 tgaattggaa cgcgagatcc cggtattgaa aaaatactat ctcacctata gcgaggtcgc
     4021 agggaaagat gttcaataca tgttctacga tctgttcagg aactatatcg aactcaaagc
     4081 cgaaacactt tcaacgtcct gttttatcaa tgacggaaag gggaattatg tccgaaccga
     4141 cctgccggat gaattgcaga aggcccctgt attttcgttt gcacaattgc ccgatagcag
     4201 ttacctggca gggggtaatt tttatggggt ccggccttat gaaggaaagt atgatgcatt
     4261 atgccccacg gtgttctcat tcgataagca cagcggccgg gcaagcgcgc gcgatatcct
     4321 tcccgatgta aacggggagg tacgggattt gaagtggttg catacggcta aatatggaga
     4381 tatcctggta gctgcccgga ataatgacag cctgttattt tttaaatata aaaagtagaa
     4441 gcgtatgacc cgtaggtttg gcttggtgat gatactggcg gccctggcgg cgggatgctc
     4501 gaaacatagc gattcctcca ataatgtcgt aactccgccg gccggcccat ttacgctgaa
     4561 cagcacgaca ctgaacggga ttgctaccgg gtcgccgttc tacaacatca acttttctcc
     4621 tgtcatccat tgttcgttct ccggccccgt ggaccatagc tccctgacca acgggtttta
     4681 ttttaaggac aataccggga catccgtcgc atggtcggtc acttatcaga acggggacag
     4741 cgtcctggtc atccagcctt catcagcctt gcaaccactt accaaatatg acttcggttt
     4801 caatacgagc ctgcagtccc agcataagat caaccttcag agcgcgagcg atgttccctt
     4861 catgacggca atcgactcaa cggacaagtt cccgaggata acggacagcg ctctgctgga
     4921 cctggtacag cgccagacgt tcacctattt ctgggacaac ggtaacgctg tatccggtct
     4981 cgcccaggaa agaacaacct ccggtagtac tgtcaccacc ggcggcagcg gttttggtgt
     5041 catggccatt ctcgtggcta ttcaacggaa tttcatcacg cgggcccagg gtcttgcccg
     5101 gatcgcgacc atcgtcaact tcctttccga ttccacgaaa gtgacacggt atcacggcgc
     5161 tttttcgcac tggatgaacg ggtccacggg cgcgacgatc ccctttagcg ccaatgacga
     5221 cggaggcgac atagtcgaga cctcctatat gatggaagga ttgctttgcg cgcgtcagta
     5281 tttcaatagt ggcaccgatc caactgagat ggggctgcgg gccaagatta atgcattata
     5341 tggcggtgtc gactggaact ggtaccggca aaacggtctg aacgtcctgt actggaactg
     5401 gagtcccgat catgcatggg ccgtcaatgt accggtgaat ggttgggacg aggccctcat
     5461 cacctatgtc ctcgctgcct ccgcgagccc ggacagtatc cccaggatcg tatacgacaa
     5521 tggctgggca cgcaatggcg ccattcaaaa caagaagagc ttctacggca ttacactgcc
     5581 gctgggcccc gactatggcg gacccttgtt ttttgcgcat tattccttcc tgggcattga
     5641 cccccgcggt ttatctgaca cctatgccaa ctattgggca caggatacgg cccattcccg
     5701 gatcaattat ttgtattgcg tgaataatcc caagaattat ttcggctata gccatctatg
     5761 ctgggggctc accgcaagcg atgaacagaa cggctactcg gcgcattccc ctacgaatga
     5821 cgatggcgtg atctctccca cagctgccat ctcctcgatg ccctatacac cgaccgagtc
     5881 tatgaatgca ctccgtttct tctactatac cctcggtgac cggctatgga aacaatatgg
     5941 tttcatcgac gccttcgatc cggccaccgg ctggtatgac accgatttcc tggccatcga
     6001 ccagggaccg gaaatcgtca tgatcgaaaa ctaccggagc gggctgctat ggaacctgtt
     6061 catgagctgc ccggagataa aagccggtat gaaacgattg ggttttagcg ggccatcact
     6121 ttaaataatg aacatgaaaa tacccgcaat catattcgca ctcgcctcca cactttctgc
     6181 catggcgcag ccggggcttt ccgattctgc ccttctggac atcgtggaaa agcagacctt
     6241 tgagtatttc tggacaggag cggaacccaa tagcgggatg gcccccgaac gcatccatat
     6301 ggacaacgtc tatcccgacc acgaccaggg tatcatcgct accggcggca gcggtttcgg
     6361 ggtgatggcg atactcgcgg gtatccaccg gcactttatt acacgcggcc agggatatgc
     6421 gcgtttggaa aagatcgttt cctttctcga aaaggccgat cggtttcacg gtgcctggcc
     6481 gcactggctg aatgatacta ctggtcatgt aaagcccttc ggcagggacg acaacggcgg
     6541 cgatctcgtg gaatcctgct atcttttgca gggcctcctg tgcgtccgcc agtatttcaa
     6601 ggatggcaac cccgaagaaa agaaactggc cgcccgtatc gacgagctat ggcgcggagt
     6661 cgatttcgac tggtaccgca acggccggca aaacgtcctc ttctggcact ggagccctac
     6721 ctatggctgg aaaatgaatt tcccggtaca tggatataac gagtgtctca tcctgtatgt
     6781 gatggcagca tgctccccca cacatacgat ccctgcggaa gtttacaatg aaggatgggc
     6841 gcagaacggc gctattcacc aggtcagctt ttataagaaa gataccctcc atttgcacat
     6901 gcagggtaat ccgccgcacg gaggcccgct attctggtcg caatattctt atctgggtct
     6961 cgacccgcat ggactgaaag acagatatgc cgattattgg aaggaaaact cgacacaatc
     7021 catcatcaac tatacctggt gtgtagacga tccgaaacac tatgccggtt acggtcccga
     7081 caattgggga ctcacggcca gctattcggt cgcaggctat gcggcgcatg cccccgatct
     7141 gaagaatgat ctcggcgtca tctcacccac cgcggccatc tcctccattc cctatacccc
     7201 acggcaatcg atggcagcga taagacactg gtataaaatg ccgggcggca agctattggg
     7261 caaatatggc ttttatgacg ccttcagcga aagggcggcc tggtatccgc cccgttatct
     7321 ggccatcgac cagggacccg aagtggccat gatcgagaac tatcgcagtg gtctgctatg
     7381 gcggctgttt atgagttgcc ctgaagtaca cgccggactg aagaaactgg gattcgaaag
     7441 cccatggata aaataaccga tatgagaaac ggattcatat tccttgcttt gctatccact
     7501 cagagcgtcc ttccgcaggc caccatccgg cccgtcggca tcatccgggg cctctccgat
     7561 tccgccctgc tcgatgttgt ccaaagacag acctttcgct atttctggga tttcgctcac
     7621 cctgtcagtg gtctcgcgcg cgaaagggac aataccgtcc gcgccgaata ctattgggat
     7681 tatatcaacg aggccgacgg ggagcccaat ttcagcaagg atactttcgg acccgaggcc
     7741 tgcgccatcg gcggtacggg tttcggcatt ctttcgacca tcgtggccgt caaccgaaaa
     7801 tggatcggca gggacactgc ccttcgcagg ctggtaaaga tcgtcgattt tctcacccat
     7861 gcggattgtt atcacggcat ctatccccat ttcatgaatg gagccacggg caggacgatc
     7921 ccctttggcc ggctcgatga tggagcagat atcgtggaga cttcctacct gatgatgggt
     7981 ctgctgacag cccgggaata cttcaacggc cacaacccgc tggaaaccta tttccgcaac
     8041 cgcgtcacgg cgatgtgggc ggatgcggac tggaactggc acagcaacaa cggcagcaag
     8101 aaactctact ggcattggag cccgcgtaac gacttcaaca tgaactttcc cgtcttcgga
     8161 tgggacgagg ccctcatcac ctatgtcatg gccgcttctt ctcaaactca ccccatcccg
     8221 aaggagctgt atgaatcctg ctgggtgggc agcgcttcct ggcgcaatgg gaaggaatat
     8281 tatggcatca aactaccact cggcaatttc gaatggggcg ggccgctgtt cttcgagcaa
     8341 tatactttta tgggcgtcga tcccaatggt ctgaaggacg atcatggcat cgattacgcc
     8401 gaacagacaa ggaatcatac gttgatcaat cgcgcctatt gcatagacaa cccgaaaaaa
     8461 ttcaaaggct atggcgccga ctgctggggc ctcaccgccg gcgacagcta taagggatat
     8521 gtcgctcatt gcccacagga cgacagaggc gtcatccagc ctacggcggc gctgtcttcc
     8581 ttcccctata cgcccgaata cagcatgcag gcgttgaaac atttttacta tgaccttggc
     8641 gacaaaattt ggggacccta tggatttgcc gacggattca gcgagtcgca caactggtat
     8701 gcgaagacgc acctggccat cgatgaggga cccatcgtgg tcatgatcga gaactatcgc
     8761 agcggcctga tctggaactt gttcatgcga attcccgacg ttcaggcggg tttgaaaaaa
     8821 cttgggttcc aatcgcccga tatgaaatga acggacagta tatcaaatat gcgctgatcg
     8881 gagcggcctg tctgatactg gcaggcactt cctatgccca ggtaaacagc gtaggcaaga
     8941 tcgtatccta tacaaagatc cccggtggga tcaccggaag gctggccggc gccatcttcg
     9001 aagtacgggc atggtctccc aacatcatcc gcgtccgggt ctccagacgc aaatccttcg
     9061 atgacttttc ctatgcgttg atccaacccg atccatcgac acaggttaaa gtcgggatcg
     9121 tggaaaaaga tgaccgtatc ctgttgtcca ccacatcgct tcatgcggag atcgaaaaaa
     9181 cacccgattt ccgtgtcact tttcggaata atgccggcgc cgtcatcaat gaagacatga
     9241 tgggggatgc gtttggtacg agctttatcg gaaacaaggt gaccatttac aaaaaactcc
     9301 aggacggcga acgtttcacg ggtctgggtg aaagtctggg cccgctggac cgacggggca
     9361 tggggatcac gctatccaac acagacaatt ataaatatgg cgatccacgg ctgccgatgt
     9421 acagcagcat cccattctat atcggtatcc atcatgacca ggtctacggt ctattctttc
     9481 acaacaccta taagagcttt tttaatttcg gtctgtccac tcccgatttt tcttcgatca
     9541 gcgccgaagg aggagatgcg gattattttt tactatacga cagcaccatc ggcggtatcc
     9601 tgaaggctta tacatctctc acaggtcgca tgcccctccc tcccctgtgg agcattggct
     9661 atcaccaatc aagatgcacc tactaccctc aggataaggc gctctggatc gcccaaactt
     9721 tccggagaaa acagatccct atcgactgca tcgtccttga cgcagattat ctgcatgagt
     9781 atgaaccttt ccggatcgac aaacgccggt tcccggatat gcgcggtctt gccggccagc
     9841 tacaaaaaat gggtatcgag ctgaccgcct ccgttaaccc gggtatcaag ctggacacta
     9901 cctatccagc gaatgccgat ggactaaaac aggatgtttt tatcaaatat accgacggcc
     9961 gccccttcgt ttcggagatc gcccccagcc tgaaccattt tgtggacttc accgatccga
    10021 aggcaagggc ctggtggatc gataacatga aattcctgcc acagaacggc atttacgggt
    10081 attggaatga catgaacgaa cctgcggtcg gcggcagcta tctgccggat aacctggtat
    10141 tcgatttcga cggcagaaag gccagcgcgg ccgaagccaa aaatgtctat ggaatggaga
    10201 tggcgcggag cagctatgag tccggtctga attacaatga tggcagacgc ccgttcgtac
    10261 tgacacgcag cgcatttgcc ggaatacaaa gatattcggc agtttggagc ggcgacaacc
    10321 aggcaaaaga cgagtatctg cttggcggcg tcctgctcaa ctgccagctg ggcttgtcgg
    10381 gtgtcccctt cgtaggcccc gatctcgggg ggtacatcgg agacgggaac aaacagctct
    10441 ataaacggtg gatcgaggtt ggtatattct ctccatttgt ccgcaatcac cgcgaatttt
    10501 ttggcgccgc taacgagccg tgggcttatg gcgaagaggg ggaagccatt tcgaagacct
    10561 atatcgggtt ccggtaccgg ctgatgcctt atatctattc ccttttttat gagacggcac
    10621 aaaccggcat gcccgtcgcc cgaagcctct gtatcgacga tccgttcgat cctaaagtct
    10681 acgatccggc ataccaatat gaattccttt ttggagatgc cctgctggta acgcccatga
    10741 cgagcaccga gacatataag cggatctggc ttccccgcgg cgactggtat gatctatggt
    10801 cggatagtct gctgacgggc aataaagagg ttggcgggga atatcccatt taccgcctgc
    10861 cacttttcgt aaaagcgtcc tcgatcctgc ccatgcaaag cctgatacaa tccacgaagc
    10921 aaaggcccgg cgacacgttg caggtacata ttttctatgg caaagaaaaa ggccggttcg
    10981 tctattatga ggacgatggt gctacctttg cctatcgaca gggacattat tgccagcgga
    11041 ccatcggatt cgatccggtg gaacaacgaa tagtattcgg gacgcaggaa ggagattatt
    11101 catcctgctt tggtcacatc gaagtcatcc tgcatggctt ccccccggtg accggttccc
    11161 gacccgatac ctgccggatc ctggatgagc tgggagagat ggaagatctg tatgaccccg
    11221 ctacattccg acagctgagg gccggactgc ccgttatccg gacacagacg atcaccctcg
    11281 ctaattcaaa agaacaattg atcattaaat ggtaataata tgaaatcctt tcaactgacc
    11341 cagcaacagc tggccgacta taacagggac ggatatctca tcgtcaggaa ttttctgaca
    11401 agcgaggaga tcgcaaaact atatgctatc gctaccggcg atgccaccct ctcaaaacac
    11461 gccttcgatc ttaacgatct caccggcaag aaaacaaaac tgaccctctg gtacaatccc
    11521 ggtgacgacg cctatgggct tctcacacgc agcaaaaaga tggtcgattc ggccaaccag
    11581 ctgatggatg gtaagtcacc tgtatgccat tttcatagca agctgatgca gaaagagccg
    11641 aaagtcggcg gcgcatggga atggcaccag gattatgggt attggtataa aaatgagttc
    11701 ctcttccccg atgagatggt gtcggtaatg atcgcgatca ctaaagccac caaagagaac
    11761 ggctgcctgc aggtgatcgg cggcacccac aaaatgggac gtatcgaaca tggattctcc
    11821 ggagaacaag tgggcgcctc gcaacattat gtcgatctgg ctctaaagac catgccgctc
    11881 gtctatgtcg agctcgagcc cggtgatgcg cttttcttcc atagcaatac ccttcaccga
    11941 agcgaggcaa acctttcgga cagaccgcgt tggtcgctca tctccgttta caaccgccag
    12001 gaaaatatcg gatacaatga accgaacggt tcgcacacca cggccgtcaa cgtcgttccc
    12061 gatgaagcgc tgctggaatg ggataccaaa ggtgtcgggg aatcatccgg ctttctgcaa
    12121 aaagaaaaag acgaggcgct gaaataaatg aagacattac tctatatgtc catcttcctc
    12181 gccgccgtgc gcggccaggc acaggaaaaa acgtattgca accccatcaa cctggattac
    12241 ggctataccc ctatccccga cttcacccaa tggggtcgtc accgcgcgac cgccgacccc
    12301 gccatggtca actataaagg agacttctat ctcttcagca cgaaccagtg gggctatt
//
LOCUS       DASYSV010000585         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_374579, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000585 DASYSV010000000
VERSION     DASYSV010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374579"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VGQ51_18235"
     CDS             complement(<1..743)
                     /locus_tag="VGQ51_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEV9038584.1"
                     /translation="MKRTWTLLLLSLAAACGSRPKSLPRIAIAGLGIESSTFSPALTD
                     EAAFHAQYDTAVFGIYPFMAKDSPLRGRAVWIPAIVGHSLPGGAVTRAAYESLLNKML
                     DSLNAHLPYDGLFFDIHGAMSVVGLDDPEGDMIKRIRKVVGPHTLISTSMDLHGNVSE
                     TLAENTDLITDYRLAPHEDAMETKQRAVEDLLERLESGKGKPRYKAWIPIPVLLPGEK
                     TSTRIEPAKSVFKAVKPTLAQEGIIDGGI"
     gene            complement(753..1355)
                     /locus_tag="VGQ51_18240"
     CDS             complement(753..1355)
                     /locus_tag="VGQ51_18240"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV9038585.1"
                     /translation="MLKKYYRFSFLFFSFALLITSSCLAQTKMLAKGDAAPVFNAQAS
                     LAGSEFDFSLKNALAKGPVVVYFYPSAYTGGCDLEAHTFAEEKDKFTAAGATIIGVSA
                     DDIQRLNSFSSDPNYCAGKFPVASDPGGKIAETYGLTFAPPRAGIKDVRGVDLTHGFI
                     PRTTFVIDKQGKIVAVFSSSTDHISPAEHVEKSLEIVKGL"
     gene            complement(1471..2724)
                     /locus_tag="VGQ51_18245"
     CDS             complement(1471..2724)
                     /locus_tag="VGQ51_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEV9038586.1"
                     /translation="MDIFVYADWKGLNGPERMGTLKVARTKGHGVFSFEYDEEWIKKG
                     RAQDLDPDLQFYPGPQYLGKDRPNFGVFMDSSPDRWGKELMRRRESILARQDSRKPNT
                     LFEEDYLLGVYDLHRMGGLRFKIPGNDHFQHDVQGLAAPPWTNLRELQHASLEFEKDM
                     LQDQQALQWINLLIAPGASLGGARPKASVTDGQGNLWIAKFPSITDQTNVGAWEMIAA
                     DLALQNDIHIAHCEVKRFNSKFDTFLSKRFDRTGEGSRLHFASAMTLLGRTDGADATT
                     GVSYLDLVQFIMEKGAAPGRDLEQLWRRIVFSIAVKNTDDHLRNHGFLLSGSGWLLSP
                     AYDINPVHFGTGLSINISEDDNSLDFDLALSVADYFRLQPSKARDIITQTKKTVGKWP
                     ALADKYQIPRQEQEQMSAAFELATL"
     gene            complement(2727..3041)
                     /locus_tag="VGQ51_18250"
     CDS             complement(2727..3041)
                     /locus_tag="VGQ51_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV9038587.1"
                     /translation="MKTRSVILLPKTQRILDQFGENIKLARLRRKLSTDQVAERAGIN
                     RTTLWNIEKGIPTVAMGAYCQVLSVLGLAGDLLKVASDDELGRRLQDADLIVKKRAPK
                     KK"
     gene            complement(3321..>4039)
                     /locus_tag="VGQ51_18255"
     CDS             complement(3321..>4039)
                     /locus_tag="VGQ51_18255"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HEV9038588.1"
                     /translation="ILVTYCTVCRTGRIYSPFVNGKYAAFRLVGMDHFNAMFEDEDTR
                     SWWRQATGKAITGPMKGQQLAEIPSAQMTLGDWQTLHPNSLTLQPDPNFKSKYDSLKG
                     YDEGTLKSGLEKRDSGSWKFKSWVIGIESGGRSKVYDWNNLVRDRVIPDTIGKRHLLL
                     TIEPDNKTFYALSYNDSLSFEYDTSAKTIRDSNTHSTWSPSGICTAGPLKGTQLQTVQ
                     AYQEFWHSWRTFHPGAVEVR"
BASE COUNT          934 a         1018 c         1043 g         1044 t
ORIGIN      
        1 cagatgccgc cgtcgataat cccttcctgc gccagtgtag gttttacggc cttgaacacg
       61 cttttggccg gctctatccg tgtgctggtc ttttccccgg gcaggagaac cgggattggt
      121 atccacgcct tatatcgcgg tttgcctttc ccgctttcca atctttccag caggtcttca
      181 acggcccgct gtttcgtctc catggcgtcc tcatgtgggg cgagccggta atccgtgatc
      241 agatcggtat tttcggccaa cgtttcggaa acattcccat gcaggtccat ggaagtagag
      301 atcagcgtat gagggccgac caccttccga atccttttga tcatatctcc ttccggatcg
      361 tccagcccaa cgacgctcat ggctccatga atatcgaaaa aaaggccgtc atatggaaga
      421 tgcgcattga gcgaatcgag cattttgttc agaagcgact cataggccgc gcgtgtaacg
      481 gcgccccccg gcaaggaatg accgacgatc gcagggatcc agacggcccg tccacgcaaa
      541 ggtgaatcct ttgccataaa aggatatatc ccgaatactg cggtatcgta ctgcgcatgg
      601 aatgcagcct catcggtgag cgcgggcgaa aaggtgcttg attcgatccc cagacctgcg
      661 atagcgatcc ggggaaggga tttgggccgg gaaccgcacg ccgcggcgag ggaaaggagg
      721 aggagggtcc aggttcgttt cacagcaaag aattaaagcc cttttacgat ctccagtgac
      781 ttttcaacgt gctcggcggg cgagatatga tcggtggatg atgaaaagac cgctacaatt
      841 ttaccttgtt tatcgatgac aaaggtggtt cgagggataa aaccgtgtgt aaggtccacg
      901 ccgcgtacgt ctttgatccc agctcttgga ggggcaaatg tcaggccgta tgtctctgca
      961 attttgcctc cggggtcaga tgctacgggg aacttgcccg cacagtaatt cgggtcggaa
     1021 gagaatgaat tcagccgttg aatatcgtct gcggacacac cgataatggt cgctcccgcc
     1081 gctgtaaatt tatccttctc ctcagcgaaa gtatgtgctt ccagatcaca gccgccggta
     1141 taggcagaag gatagaaata gaccacgacc ggccccttag caagggcgtt ctttaatgaa
     1201 aaatcgaatt cactgccggc cagactggcc tgagcgttaa atacgggcgc ggcatctcct
     1261 tttgccaaca tcttcgtttg ggcaaggcaa gacgaggtta taaggagcgc aaagctgaaa
     1321 aataaaaaag agaagcgata atatttcttt aacatggcgg attgttttga cttaggaata
     1381 gcgttgttat caaatttact ataatctgca acgctgcagc ccggaagtca actaaatttt
     1441 accgtagcgg ccgggcgggt aaatgcgata ttacagggtt gccagttcga atgcggccga
     1501 catttgctcc tgttcctgtc gcgggatctg gtatttatcg gctaatgccg gccacttgcc
     1561 taccgttttt ttagtttgcg tgataatatc ccgtgctttc gacggctgaa ggcgaaagta
     1621 atccgctact gatagtgcga gatcaaaatc gagagaattg tcatcctcag aaatattgat
     1681 cgatagtccg gtaccaaaat ggacgggatt aatatcgtat gccggagaaa gcaaccatcc
     1741 ggaaccggaa agcaaaaatc cgtgattgcg aaggtgatca tccgtatttt ttacggcaat
     1801 tgaaaagacg atccgacgcc acaattgttc aaggtccctg ccgggagcgg cgcctttctc
     1861 cattataaat tgtaccaggt ccagatagga aacccccgtc gtagcgtccg ctccatctgt
     1921 tcttcccagt aaagtcatgg ccgatgcaaa atggagccgg cttccttcgc cggtgcggtc
     1981 aaatctcttt gagagaaatg tatcgaattt gctattgaac ctcttcactt cgcaatgggc
     2041 gatatgaata tcgttttgca gggcaaggtc cgctgcgatc atttcccagg ctcctacatt
     2101 ggtctggtcg gtgatacttg gaaatttggc gatccacagg ttgccctgcc cgtcggttac
     2161 gctggctttg ggcctggcgc ctcccaaaga tgcgccgggt gctattagca ggttgatcca
     2221 ctgcaatgcc tgttggtcct gcagcatgtc cttttcaaat tccagacttg catgttgcaa
     2281 ttcacggagg tttgtccagg gtggagcggc caggccctga acatcatgct gaaaatggtc
     2341 gttaccaggt attttaaatc gcaagccgcc catccggtgg agatcataaa cacctaataa
     2401 atagtcctct tcaaataatg tattgggttt acgcgaatcc tgacgagcca ggatcgactc
     2461 gcgtctgcgc atcagctctt taccccaccg atcgggagaa gagtccatga aaacaccgaa
     2521 attgggcctg tcctttccca aatattgggg tccgggataa aactgaagat ccggatcgag
     2581 gtcctgcgct ctgcctttct ttatccattc ttcatcatat tcaaacgaaa atactccatg
     2641 tcctttggtg cgcgccacct tcagtgtccc cattctctca gggccattta atcctttcca
     2701 atctgcatat acgaagatgt ccattcctat ttctttttgg gagcacgttt ttttacgatc
     2761 agatcggcat cctgcaggcg gcgtccgagt tcgtcgtcgg atgcaacctt taataaatcc
     2821 ccggcaagac cgaggacaga cagtacctgg caataagcgc ccatggcaac cgtaggtatt
     2881 cccttttcga tattccagag agtggtgcgg ttaatacccg ccctttcggc tacctggtct
     2941 gtggacaact tgcggcgaag tcgggccagc ttgatgttct cgccaaactg gtcaagaatt
     3001 cgttgggttt tggggagtaa aatgactgac ctggtcttca taatgttgat tgttgtgaac
     3061 aaatataggc aatttgttta ttatagtcaa cgttgtaaat gagaagagtg ggcgtttttc
     3121 tccggcgatc tacgtaaatg cctgataatc atggctcgcg atcgggatgc gaaaatgggg
     3181 tttgtgcttt tatttgatca aggagtcgtg atgggtcgtc cgaaaaacag tacgtatcca
     3241 tcggcatcgg ccagctcgaa cccccgcaat ccgtcctgat tgtctgcgag gggttgacga
     3301 aacgccagtt aaaatatcta tcacctaacc tcgacggccc cgggatgaaa ggtccgccat
     3361 gaatgccaga attcctggta ggcctgtacg gtttgtagtt gcgtaccctt cagcggcccg
     3421 gctgtacaga taccgcttgg cgaccatgtg ctgtgggtat tgctatcccg gatggtcttc
     3481 gcgctggtgt catattcgaa gctcaggctg tcgttatagg ataaagcata aaaggtttta
     3541 ttatcaggtt cgatcgtaag taacaggtgc cgcttgccga tagtgtccgg gataaccctg
     3601 tccctgacca ggttgttcca gtcatacact ttgcttctcc cgcccgactc gatgccgatg
     3661 acccaggatt taaatttcca ggagccggaa tctcttttct cgaggccgct tttgagcgtg
     3721 ccttcgtcat agcctttcag actgtcgtat ttgcttttaa aattcggatc gggttgcaag
     3781 gtcaggctat tggggtgcag tgtctgccag tcacccagcg tcatttgggc ggaagggatc
     3841 tctgccagct gctgcccctt catagggccg gtaatagctt ttcctgtggc ttgccgccac
     3901 cagctgcgtg tgtcctcatc ttcgaacata gcgttgaaat gatccatgcc cacgagtcgg
     3961 aaagcagcat actttccgtt tacaaacggg ctatagatcc tcccggtccg gcagaccgtg
     4021 caataggtga ccaggatcg
//
LOCUS       DASYSV010000586         7207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1650890, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000586 DASYSV010000000
VERSION     DASYSV010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650890"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VGQ51_18260"
     CDS             <1..351
                     /locus_tag="VGQ51_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038589.1"
                     /translation="GDAKAALSADQHLSFIKQCEAYIGQLKAAAILIAAQPIAREGAT
                     LSKKKDGWNMALIDPGKEVQVGYYHILADDFEEAVAIAKRNPEFEYVPSASIEIRPVK
                     VKEAETGFVYPQGM"
     gene            664..1263
                     /locus_tag="VGQ51_18265"
     CDS             664..1263
                     /locus_tag="VGQ51_18265"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV9038590.1"
                     /translation="MYTYSFPGKSISLLALFPFLFYLTANSQVRYISTTANLTINGTS
                     TLHDWTMTSVKVQCDATFELTPTGQITAVRSISFSTPVNALKSEHSAMDNNAYKALKS
                     DKDPQISFTATSVTIAPAPAGASQVTVTGKLNIAGATRDAQILAVCQPNLSDNTITVS
                     GKENISMKDFSIVPPTFMLGTIKTGNDITLSFKLTLKRA"
     gene            1286..1693
                     /locus_tag="VGQ51_18270"
     CDS             1286..1693
                     /locus_tag="VGQ51_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038591.1"
                     /translation="MKKIVTSGLIAGIVLLTLSVLGLYATIWLFPTVAEQYYDPSFDN
                     ESGRYMIYYAHPFVIAFALAWFWDRFKGALKGSFLTKGIEFGLIYALVAIFPAMWITY
                     SALSVSLTMVATWLAFGLLQGLVAGLVFEKTNP"
     gene            1707..2315
                     /locus_tag="VGQ51_18275"
     CDS             1707..2315
                     /locus_tag="VGQ51_18275"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV9038592.1"
                     /translation="MKPNVLLLLLVLLLTSAGDPSRDLYVLSAEHTVTITGTSNLRDW
                     KEDVCQVSGAVEAEVNPDGTISIASIRITMNVLSIKSDMGRVMDNKTYDALKARTYPK
                     ILFTLSAPLTLNPVRDCGISIPVKGSLSLAGVCRPVTMEVNTFQITRGELQFEGYQRL
                     KMTDFGVTPPSALFGTMHASPDITIHFKTNFISQTILNNLKN"
     gene            2318..3700
                     /locus_tag="VGQ51_18280"
     CDS             2318..3700
                     /locus_tag="VGQ51_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038593.1"
                     /translation="MKTLNSLTRSINAVRQILPIILLASALTAAAQDGHMQYYRPNDK
                     TGLNVFETTKNDTTSFRKLHVKVGGNFEETFQNLNNKNTASSMTMNGYSGNVNSLEDL
                     TPGFDLAMANLNLDVQLADGIRVNLTSYLASRHHEDAWVKGGYIQFDKLLFLHSSLID
                     NIMKSVTIKIGQFDVNYGDQVFRRTDGGNSIYNPFIENYIMDEFATEIGGEVIYHCKS
                     GLFALGGITNGELNPTILAATKVDSATGQLNKYDPAWHGKLGYDKQLTKDFRLRLSQS
                     FYIDKSANSNTLFGGDRTGSHYYGVMSNAAIAKGTTLNNANDYNPFSGRLNPGFSEEV
                     NAYMSNAFLKFKGLELFGTYENAKGRTSTEKTQRQATQWAFDAIYRFTRNENFWVGFR
                     YNTVNAALPGSTSNVTIEREAGSVGWFLNHNVMLKLEYVNQVYMGYPSTNILNGGKFY
                     GWCAEASIAF"
     gene            3830..4204
                     /locus_tag="VGQ51_18285"
     CDS             3830..4204
                     /locus_tag="VGQ51_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038594.1"
                     /translation="MGAHIMKSHVSRPFLREVENEFNRIYPYLRIEFPGQGSESSNPG
                     DPHAEAALRQKAGDALRDDARLADSMTVSELETSLQVLFALPVQIFRKSGRLWIETKM
                     TRSWTLKQQNDRGRELAAGEEG"
     gene            4232..5053
                     /locus_tag="VGQ51_18290"
     CDS             4232..5053
                     /locus_tag="VGQ51_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV9038595.1"
                     /translation="MKQVLFVCAGKEFPKGAFNFLLSMQQDAPVEALGLFFSPIDLEA
                     MVTVTQFPVQGPFYRLEEEEKQTVMANKAIFAKQCEQHHIRHRIHTNDQQWDKHLLVN
                     ESRFADLILLSGELFYADINLRQPNSYLQEALHSAECPVLVIPEDFRQCDQLFIAYDG
                     SKESLYAIKQFSYLFPNYSELPSEIVYIRDEASDEIPDLDHLKYYTRLHFSAVGFSKL
                     HFKAAHYFASWISEKKHVLLITGCYSRSPFSYVAKHSFGEQVIHDHRLPVFIAHP"
     gene            5098..>7207
                     /gene="mgtA"
                     /locus_tag="VGQ51_18295"
     CDS             5098..>7207
                     /gene="mgtA"
                     /locus_tag="VGQ51_18295"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEV9038596.1"
                     /translation="MQNTTSKAPALKNKQLLEAISFPLATLKDRLQFSEKGLSTTVAE
                     ERLKTYGHNEIAREKPPSPFRRLWLHLKNPLVILLSILGAASWLTGDIRATIVIFIMV
                     ILGVVLRYFQEMRADNAAQKLKAMVSTHATVFRDGKQCELPLRLLVPGDLVILAAGDM
                     IPADLRILSAKDLFINQATLTGESLPVEKNEKAMPNPSGNPLEVHHCCFLGTNVVSGS
                     ATALVVTTGSHTYFGALAHSIIGDRPPTSFDKGINRFTWLMIRFIFIMVPAVFIINGF
                     SRHDWLEAFLFSLAVAVGLTPEMLPMIVSVNLSKGAIAMSRKKVIVKRLNAIQNFGAM
                     DILCTDKTGTITRGKIVLEQHLDVHGNSSTKVLEYGYLNSCYHTGLKNILDDAVLGCE
                     EEASAEKLCANFGKVDEIPFDFQRKRMSVVVCEKDGPDLLICKGAVEGLLDVCTQIDT
                     DNDLKPLTESDKTASLQLVHKLNSKGFRVVALAYRPIERSPDGPHYEACDEKDLTLLG
                     FLSFFDPPKESAEQALARLKKLHVEVKILTGDNESIASYICQKVGMPTDKILLGPDID
                     QLDDHQLATAAGDNTIFARLVPLQKQRIIQALQQNGHVVGFMGDGINDAPALKAADIG
                     ISVDSAVDVAKESSDIILLENSLLVLEQGVAEGRSVFGNIVKYIKMSASSNFGNMFTV
                     VGASAFLPFLPMLPIQVLTNN"
BASE COUNT         1830 a         2258 c         1599 g         1520 t
ORIGIN      
        1 ggcgacgcca aagcggcatt gagcgccgac caacatcttt cattcatcaa acaatgtgag
       61 gcatacattg gccagttgaa agccgctgca attctgatcg cggcccagcc tatcgcccgg
      121 gaaggcgcga ccctttccaa aaagaaagac ggatggaata tggcgctcat tgatccgggc
      181 aaagaagtgc aggtcggtta ctatcatatc ctcgcggatg attttgaaga agccgtcgcg
      241 atcgccaaac ggaatccgga attcgagtat gtgccttcgg cgagcatcga gatacgtccc
      301 gtaaaagtga aagaggccga aaccggcttt gtatacccgc agggcatgtg agcgcgggcc
      361 ctccctcgtt ttagttcact caaaatcctc ccgacggcct gacggacaaa aagttccgtt
      421 cctcgctcct ttggcccaaa tattcaaatc ctcaccggtc attccccaaa attgatcccg
      481 gctctcctga ccaatcttct cccccctcac acctggcttc cctctccccc cacctgcgtc
      541 aaatcatctc cgccgctgac caacgtcacc tccgccggtc accaacatca tctccgcgcg
      601 ctacgatcgt catcttcccc tgccccatcg gcgaatactt ttacaccatc aaaatctatc
      661 gccatgtaca catattcatt cccggggaaa tcgatatcgc tcctggccct attccccttc
      721 ctcttctacc ttacagccaa ttcccaggtc cgttacatca gcaccaccgc caacctgacc
      781 atcaacggta cctcgacctt gcatgactgg accatgacct cggtcaaagt ccaatgcgat
      841 gccacattcg agcttacccc gaccggtcag atcaccgctg tccggtccat ctccttctcc
      901 accccggtca atgcactgaa aagcgagcac tccgccatgg acaataacgc ctacaaagca
      961 ctcaagtccg acaaagaccc acagatcagc tttaccgcca cctccgtgac aatcgcaccc
     1021 gccccggcag gcgcaagcca ggtcaccgtc accggcaaat tgaatatcgc cggtgcaacc
     1081 cgtgatgcgc agatcctggc cgtctgtcag cccaacctgt ccgacaacac tatcactgtg
     1141 tccggcaaag agaacatcag catgaaagac ttcagcatcg ttcccccaac cttcatgctt
     1201 ggtaccataa agacaggtaa tgacattact ctttcgttca aactaaccct caaacgcgcc
     1261 taaccacctt aataaaaatc aacacatgaa aaagatcgtt acatcgggat tgatagcagg
     1321 catagtgcta ctcaccctca gcgttctggg actatacgcc accatctggc tcttccccac
     1381 cgtcgcagag caatattatg atccctcttt cgacaacgag tccggccgct atatgatcta
     1441 ttatgcgcat cccttcgtga tcgcgttcgc actcgcctgg ttctgggatc gcttcaaggg
     1501 cgcgctgaag ggatctttcc tcacaaaggg catcgaattc ggactgatct acgctcttgt
     1561 cgccatcttt cccgccatgt ggattactta tagcgcctta agcgtatcgc tcacaatggt
     1621 agccacctgg ctggcattcg gccttctgca gggacttgtc gcaggcctcg tatttgaaaa
     1681 gacaaacccc tgataaacga agcgcaatga aaccaaatgt cttattacta ctcctcgttc
     1741 tcctgctgac cagcgccggc gatccgtcac gggatctgta cgtcctctcg gcagaacaca
     1801 cggtcaccat caccggtaca tcgaacctga gggattggaa agaggatgtt tgccaggttt
     1861 ccggtgccgt ggaagccgaa gtaaatccgg acggcaccat cagcattgcc tcgatccgta
     1921 tcactatgaa tgtcctttcc atcaagagcg acatgggtcg ggtaatggac aataagacat
     1981 acgacgcgct taaagcaagg acgtatccca aaattctctt cacactgtcc gccccgctga
     2041 cactcaaccc ggtaagagac tgcggcattt ccatccccgt gaaaggcagc ctgtcgcttg
     2101 caggcgtctg ccggccggtg accatggagg tcaacacctt tcagatcacc cgtggagaac
     2161 tacaattcga aggataccaa cgtttaaaaa tgaccgattt cggtgtgacg cccccctcgg
     2221 cccttttcgg gacgatgcat gcctcacccg atatcaccat acatttcaaa acaaatttta
     2281 ttagccagac aattcttaac aatttaaaaa attgacaatg aaaaccctca acagcctcac
     2341 acgttcaatc aacgctgttc ggcaaatact cccgattatc ctgctggcct ccgccctcac
     2401 tgcggcggca caggatgggc atatgcaata ttaccgcccc aacgacaaga cgggtctcaa
     2461 cgtcttcgaa acgaccaaga acgacacgac ttccttccgt aagctccacg tcaaggtcgg
     2521 cggcaatttc gaagagacct ttcagaacct gaacaacaag aacacggcgt catcgatgac
     2581 gatgaacgga tactccggta acgtcaacag cctcgaggac ctgacacccg gtttcgacct
     2641 tgcgatggct aacctgaatc tcgacgtcca actcgcggac ggtatccggg taaaccttac
     2701 cagctacctg gcttccagac accatgaaga tgcctgggta aaaggcgggt atatccagtt
     2761 cgacaaactg ctcttcctgc acagtagcct catcgacaat atcatgaaga gcgttaccat
     2821 caagatcgga caattcgatg tcaactatgg cgatcaggta tttcgccgga ccgatggcgg
     2881 taactccatc tacaaccctt tcatcgagaa ctatatcatg gacgaattcg ccaccgagat
     2941 cggcggtgag gtcatctatc attgcaagag cggattgttt gctctcggcg gaatcactaa
     3001 cggcgaacta aacccaacga tccttgccgc tacaaaggtc gactccgcca ccggtcagct
     3061 gaacaaatat gaccccgcct ggcatggcaa gctcggctat gacaaacagc tcacgaaaga
     3121 tttccggttg cgcctcagcc agagctttta tatcgacaag agcgccaata gcaacaccct
     3181 attcggtggc gaccgcacgg gctcccatta ctacggcgtc atgtcgaatg ccgcaattgc
     3241 caaaggcact acgctcaata atgcgaatga ctataacccg ttctcgggcc gtctcaaccc
     3301 cggcttcagc gaagaggtca atgcgtatat gagcaatgcc ttcctgaaat tcaaaggtct
     3361 ggaactcttc ggtacctatg agaatgccaa aggccgcacc agcacggaaa aaactcaacg
     3421 ccaggccacg caatgggcat tcgacgccat ctatcggttt accaggaacg agaacttctg
     3481 ggtgggtttc cgctacaata ccgtaaacgc cgccctgccg ggttcgacca gcaatgtcac
     3541 tatcgaacgc gaagccggta gcgtaggctg gttcctgaat cataacgtca tgctcaagct
     3601 cgaatatgtc aaccaggtct atatgggcta tccgagtacc aatatcctca atggcggtaa
     3661 gttctacgga tggtgcgccg aagcctccat cgccttttaa ttgccccgcc aggccctttt
     3721 gacaacaaga tcaacccgga ggaagcggcc accgtaccca atatgacaaa tatcatgtcg
     3781 ccggctgtca tccgtcatgt tctgaatggt agtataaaat atttttgtta tgggagcgca
     3841 tattatgaaa tcgcatgtca gcaggccatt cctccgggaa gtagagaacg aattcaatag
     3901 gatctatccc tatttgagaa ttgagtttcc cgggcaaggg agcgaatcgt ccaatccggg
     3961 agacccccac gcagaagcag ctctacggca aaaggccggt gacgccctcc gggacgacgc
     4021 cagattggct gatagcatga cggtcagcga actggagacc tcccttcagg tcttattcgc
     4081 cttacccgtg cagattttcc gcaaaagcgg tcgcctctgg attgaaacaa aaatgacccg
     4141 cagctggacc cttaaacaac agaacgaccg cggccgcgaa ttggccgccg gcgaggaagg
     4201 atgactctta cacaccataa attcattttt tatgaaacaa gtattgttcg tgtgcgctgg
     4261 taaggaattc ccaaaaggag ccttcaactt cctgctctcc atgcagcagg acgcgccggt
     4321 cgaagccctg ggcctcttct tcagccctat cgatctcgaa gccatggtca cggtcaccca
     4381 gttccccgtc cagggccctt tctaccgtct cgaagaagaa gaaaagcaaa cggtaatggc
     4441 caacaaagcc atattcgcca aacaatgcga acagcatcat atccggcatc gcatccatac
     4501 caacgaccag cagtgggata agcacctgct cgtgaacgaa agccggttcg ccgatcttat
     4561 cctcctgagc ggcgaacttt tctatgccga catcaacctc cggcaaccca attcctacct
     4621 gcaagaggcg ctccattcgg ccgagtgtcc cgtcctcgtc atcccagaag acttccgcca
     4681 atgtgatcaa ctcttcatcg cctatgacgg cagcaaggaa agcctttacg ccatcaaaca
     4741 attcagctat ctcttcccga actattccga actgccctcc gagatcgtat atatccggga
     4801 cgaagcatcg gacgagatcc ccgatctcga tcacctgaaa tattacacca gactgcattt
     4861 cagtgccgtc ggattctcca agctgcactt caaggcggca cactatttcg cctcctggat
     4921 cagcgaaaag aaacatgtcc tgctgatcac gggttgctac agccgatccc cattttctta
     4981 tgttgccaaa cacagcttcg gcgaacaggt catccatgat caccggctgc cggtattcat
     5041 tgcgcatccc tgagcaggca tgccgcatcg gccagcgaca cccaacagcc ggacaccatg
     5101 caaaacacaa cgtcaaaggc tcccgcatta aaaaacaagc agctcctcga agcgatctcc
     5161 tttcccttag ccacgctaaa ggatcgactg caattctcgg aaaagggtct ctccaccacc
     5221 gtcgccgaag aaagactcaa aacctacggc cacaacgaga tcgcccgcga aaaaccgccc
     5281 tctccgttcc gccgcctttg gctccatttg aaaaaccccc tcgtcatcct gctttccatc
     5341 ctaggagccg catcctggct caccggcgat atccgcgcca ccatcgtcat cttcatcatg
     5401 gtgatcctgg gcgtcgtact gcgctatttc caggaaatgc gggccgataa cgcagcccaa
     5461 aagctcaagg ccatggtgag cacacatgcg accgtctttc gggacggcaa acaatgcgag
     5521 cttcccctcc ggctgctcgt cccgggcgac ctggtgatac tcgccgccgg cgatatgatc
     5581 ccggccgacc tgcgaatact gtccgcaaag gacctcttca tcaaccaggc caccctcacc
     5641 ggggaatctc tccccgtgga aaaaaacgaa aaggcgatgc ccaacccatc cggaaacccc
     5701 ctcgaagtcc atcactgctg tttcctcgga accaatgtgg tcagcggctc tgccaccgcc
     5761 ctggtagtca ccacaggaag ccatacctat ttcggcgcac tcgcacacag catcatcggc
     5821 gaccgtccgc cgaccagttt cgacaaaggc attaaccgct tcacctggct gatgatccgg
     5881 ttcatcttca tcatggtgcc tgccgtcttt atcatcaacg ggttctcgag acacgactgg
     5941 ctcgaagcgt tccttttctc cctggcggtt gccgtagggc ttacccccga aatgctgccc
     6001 atgatcgtct ccgtcaacct ctccaaaggc gccatcgcca tgtctcggaa aaaagtcatc
     6061 gtcaaacggc tcaacgccat ccagaacttc ggggccatgg atatcctgtg caccgacaag
     6121 accgggacca tcacccgtgg caagatcgtt ctggaacaac acctcgacgt tcacggcaac
     6181 agcagcacaa aagtccttga atacggatac ctgaacagct gctatcatac gggtctcaaa
     6241 aacattctcg atgacgccgt cctcgggtgc gaagaggagg catcggcaga aaaactctgc
     6301 gctaacttcg gcaaggtgga cgagataccc ttcgacttcc agcgcaaacg catgtccgta
     6361 gtcgtctgcg aaaaggacgg tcccgatctt ctcatttgta aaggtgccgt cgaaggcttg
     6421 ctcgatgtct gcacgcaaat cgacaccgac aacgatttga agcctctcac ggaatccgac
     6481 aaaacagcca gcctgcagct cgtccacaaa ctcaacagca aaggcttccg cgttgtcgca
     6541 ctggcctacc ggcccataga aagaagtccc gacggcccgc attatgaagc ctgcgacgaa
     6601 aaagacctta ctctccttgg gttcctctct tttttcgatc ccccaaagga gtctgccgaa
     6661 caagccctcg caagactgaa aaaattacac gtagaagtca agatcctcac cggcgacaat
     6721 gaatccatcg cctcctatat ttgccaaaaa gtgggaatgc ccacagataa gatccttctc
     6781 gggcccgata tcgatcaact ggacgaccat caactggcaa cagccgcggg cgataatacc
     6841 atcttcgccc ggctcgttcc cctccaaaaa caacgcatca tacaggcatt acaacaaaat
     6901 ggccatgtcg tcggtttcat gggcgatggt atcaacgacg cccccgccct caaagctgcc
     6961 gatatcggca tctcggtgga cagcgccgtg gatgtagcaa aggaatcttc cgatatcatc
     7021 ctgctggaaa acagcctgct cgtactcgag cagggtgtgg cagaaggccg cagcgttttc
     7081 ggcaacatcg tcaaatacat caaaatgtcc gccagctcga atttcggcaa catgttcacc
     7141 gtcgtcggcg ccagcgcctt ccttcccttc cttcctatgt tgcccatcca ggtcctcacc
     7201 aacaatt
//
LOCUS       DASYSV010000587         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_501216, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000587 DASYSV010000000
VERSION     DASYSV010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501216"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..384
                     /locus_tag="VGQ51_18300"
     CDS             70..384
                     /locus_tag="VGQ51_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV9038597.1"
                     /translation="MDRIGKLKEFLAAEPDDPFIKHALALEYQKLGDLSGARRLWEEL
                     LEHDPGAVGSYYHLGKLLEATGEKALALGWYEKGMAAAKAADQRRAFNELKAAYDDLA
                     DE"
     gene            429..>1720
                     /gene="tilS"
                     /locus_tag="VGQ51_18305"
     CDS             429..>1720
                     /gene="tilS"
                     /locus_tag="VGQ51_18305"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV9038598.1"
                     /translation="MDLLQNFHDFIEKEKLFTAQDPLLLAVSGGVDSVALCELCHLSG
                     LTFVIAHCNFGLRGAESLRDEEFVRRLADRYHRDIRVKTFDTKAYAADRQLSVQAAAR
                     ELRYKWFSELIGEGIARNVVTAHQLDDNVETLLMNFFKGTGIAGLRAMLPRQGIVMRP
                     LLFATRADILEFVEGRGLVWVEDSSNLEDKYTRNFFRHQLIPLVEKAYPAATRNLAGN
                     IGRFRAIELIYRESIERQKKKLLEYRGPELYISVGRLKQASAPETLVYEITAPYGFSP
                     HQAEAIAGLMDSASGRFILSDTHRILKDRRWLIISPLGGVEAGIVPVEEGDREVVFAQ
                     GILRLERGIGAAVLDQGPRVAVLDASTVRYPLLLRKWKPGDYFYPLGMRKKKKLARLF
                     IDSKLSVAEKEKVWVLESDKRILWVIGQRMDDRCRVGP"
BASE COUNT          381 a          393 c          555 g          391 t
ORIGIN      
        1 ctgatgcaaa ccagtatctt cattacttgt tggttttgaa ggtgctaatt tataaaaaaa
       61 atcacgggta tggacaggat cgggaaattg aaagaatttc tggcggccga acccgacgat
      121 ccctttataa aacatgcgct ggccctcgaa taccagaagc tcggggacct ttcaggcgcc
      181 aggcgtcttt gggaagagct cctggaacac gatccggggg cggtcggctc gtattatcat
      241 ctgggtaagt tgctggaggc taccggcgag aaagcgttgg cgctcggttg gtatgaaaag
      301 ggaatggcgg cggcaaaagc ggcagatcag cggcgggcct ttaatgaatt gaaggcggct
      361 tatgacgatc tggcggacga ataacgaggc aagggcatgc gctgtgtgaa aatatgtaga
      421 tttgccggat ggacctgttg cagaacttcc atgatttcat cgaaaaggaa aagcttttta
      481 cggcgcagga cccgctactc ctggccgtca gcgggggggt ggactctgtc gccttatgtg
      541 agctttgtca tctttccggg ttaacttttg tcatagccca ttgtaatttc ggacttcgcg
      601 gggcagagag cctgcgggat gaagagttcg tccgtcgcct ggcggatagg tatcaccggg
      661 atatacgggt gaagaccttc gatacaaagg cttatgccgc cgaccgtcaa ttgtcggtgc
      721 aggcggcagc cagggaacta cgatataaat ggttcagcga gcttataggg gaggggattg
      781 cccggaatgt cgtaacagcg catcagctgg acgacaacgt ggagaccctg cttatgaatt
      841 tcttcaaggg tacgggtatc gccggactgc gcgccatgct tccgcgtcag gggatcgtga
      901 tgcgtccgtt gctttttgcg acacgggccg atatcctcga attcgtcgaa gggcggggtc
      961 ttgtctgggt ggaggacagc tccaatctcg aggataagta tacccgcaat ttttttcgtc
     1021 accagctgat cccgctggtc gaaaaggctt atccggccgc gacccggaac ctggcgggga
     1081 atatcgggcg tttcagggcg atagaactca tttaccggga gtcgatcgag cggcagaaaa
     1141 agaaattgct ggaataccgg ggcccggaat tgtatatctc cgtggggagg ctgaaacagg
     1201 catctgcgcc ggagacgttg gtctatgaga tcaccgcgcc ctacggattt tcgccgcatc
     1261 aggcggaggc catcgccgga ttgatggaca gcgcatcggg aaggttcatt ctttcggaca
     1321 cgcaccggat attgaaggat cgccgctggc tgatcatatc gcccttgggc ggggttgagg
     1381 cgggcatcgt gccggtagag gaaggggatc gtgaggtcgt tttcgcacag gggatattgc
     1441 ggctggaacg ggggatcgga gccgcggttt tagaccaggg accaagggtg gctgtattgg
     1501 atgcgtcgac cgttcgttat ccgctgctcc tgcgcaaatg gaaacccggc gattatttct
     1561 atccattggg gatgcggaaa aaaaagaagc tggcgcgttt gttcatcgat agcaagctat
     1621 cggtcgccga gaaggagaag gtctgggttc tggagtcgga taaacgcatc ctttgggtca
     1681 tcgggcagcg tatggatgac cgatgccgtg tgggtcctca
//
LOCUS       DASYSV010000588         6906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_501289, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000588 DASYSV010000000
VERSION     DASYSV010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501289"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGQ51_18310"
     CDS             complement(<1..364)
                     /locus_tag="VGQ51_18310"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV9038599.1"
                     /translation="MKKTIDPSILYFGTPVVLISTSNGDGTDNLAPISSIFWLGWRAM
                     IGISAFSKTTENLLRSRECVLNLPSASQVAAVNRLALTTGSNPVPEGKQQKGYRYEAD
                     KFGAAGLTPRPAGLVKTSV"
     gene            complement(500..1612)
                     /locus_tag="VGQ51_18315"
     CDS             complement(500..1612)
                     /locus_tag="VGQ51_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038600.1"
                     /translation="MKKMMLVAVCLTAITLFVSLIANAQDDMNEEINPGVWSAYINKD
                     RVHIQFGGIHWSSGANFDLSELGTLPTGTAGTVTVKRDPGTMTFTGNWSNGAGRGTYV
                     FVADPAFKSYLESEGFREIPDALMIHLFFTNINKAYFAYMKENGYQGITIGQLKDLAY
                     QNMNQHVMSAYVELFKKEGYGKVSLEKMVELREHGVGADFINSLHEAGYAHVSLEKAQ
                     EAMDHGVTPDFISDMKKAGARDISLDEAIELRDHGVTVEFIKGFRDLGYTDLSLERAQ
                     ELQDHGVTVEFIREFQKMGYKNVSPDRAQELRDHGVEPSFISGIKGLGFADVSLEKAV
                     ELQDHGVTVEFIRRMQEKGLKNLSLDEYVRLRDSGM"
     gene            complement(1623..3251)
                     /locus_tag="VGQ51_18320"
     CDS             complement(1623..3251)
                     /locus_tag="VGQ51_18320"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEV9038601.1"
                     /translation="MHFIPDTIIRALCWTLLHSLWQGLLFAGVAGVVLVVTRKSHAAL
                     RYNLLCGGLLLFLVVSGYTFYRELGMSVVVETGRVTVDAGVKGGHGVTAAPDAGATFV
                     SRSGVTAGIDALVGYFNTHAALVVLIWGIVFLARWVQLLSGWVYAQRIRHYKNQPAPE
                     EWQQRLGRLLEKLGIARPVSLLESALVKVPVVVGMLKPVILVPMGMLVHLSADQVESI
                     LLHELAHIRRKDYLFNFIQHVIDTMFFFNPALRWISSLIRAERENCCDDIAIRETGSR
                     RRLIEALVSFHEYGQTTGCALGFAAKENQVVRRVKRIVEKTNHSLNAGERIVLMGGIM
                     VLSAAFVTIHNSRTEKTRVVAETRVVAKAQPEVVTVVRHFNAKNVDRRHKAIVDTVPK
                     EAKQKAMAQKDTVPKEDIDKLIEARDHGVTAEFIAGFDKMGYKHISIDKAIELADHGV
                     STDFIAGIIAEGYAAPISLDLALELKDHGVTVGFIRELHGLGFPAISLEKAIELVDHG
                     VTIGFISSWKKKIGKLLEPDDYIKLRDAGISPTS"
     gene            complement(3262..3645)
                     /locus_tag="VGQ51_18325"
     CDS             complement(3262..3645)
                     /locus_tag="VGQ51_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEV9038602.1"
                     /translation="MENVEPTRAQLEILQVLWKYGPSTVRFVNDQLNRQQNQSLSYTS
                     TLKLMQIMHEKGLVHRDESNMTHVYSALLEEGKTKSIVLKKFVDSVYEGSAKSLMLEL
                     LGNEKMNEKDMDMIKKMLDRLDEDQ"
     gene            3816..4442
                     /locus_tag="VGQ51_18330"
     CDS             3816..4442
                     /locus_tag="VGQ51_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEV9038603.1"
                     /translation="MYTFDPGEGIVNAADIKRLLRKDRLFGGIYKKYGAPPPWYREPG
                     FISLSMIILEQQVSLASAKAHFQKLHGYIGEFTPSNILRLTDEEMRTCQISRQKAKYL
                     RALSDAITGNALDLHGMATLHESAIREKLTGIKGIGRWTSDIYLMFCLQARDVFPIGD
                     IAIVNTVKELTDARTQEEILTLSESWRPLRSLATYFLWHNYLSKRGRS"
     gene            4515..5228
                     /locus_tag="VGQ51_18335"
     CDS             4515..5228
                     /locus_tag="VGQ51_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038604.1"
                     /translation="MQQFTVSEAGYKKYSRNSRRVLYGFASIVAAGLLYLLVLAIEKG
                     KFDMTMIIPPCVVAVALSFQIPRILRRQKKMVMSYTINLSDHEITREQVDTRPLTINF
                     MEIKEITKTRKGHFMIKGLTRTDYIVIPNWIDNYPQLEKQLQTLAPIEEKTKEHRYAT
                     VRALLSMISIPLMVGSFLVKDKAVAAIFAVLAVGLLGWIYYETRVSKNMPENVRRRSW
                     TYLIMIAAIIYITWQKFTA"
     gene            complement(5240..5731)
                     /locus_tag="VGQ51_18340"
     CDS             complement(5240..5731)
                     /locus_tag="VGQ51_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038605.1"
                     /translation="METGAGGEHLTYEQIKEAVLEWETMLCPKMRKYIHYRTMRNFVL
                     HFHEIESEQEKDEALMILGEYVQCVKHYDFSFEGKASAQLALGYLDPLIVSYRTHSAF
                     MPVLKLRDALVLGLVIDGVLYLLGLLAALRHIPITTISLFLYYGFLVVFKIPRGRVYG
                     LFY"
     gene            6085..6471
                     /locus_tag="VGQ51_18345"
     CDS             6085..6471
                     /locus_tag="VGQ51_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV9038606.1"
                     /translation="MKKTRIIYWVLTIVFAGFMAFTAIPDALVVPDAAKFMTHLGYPN
                     YFTHFIGIAKLLGAIAILIPGFRTIKEWAYAGLFFDLIGAFYSNFMVDGANAPMVVTM
                     VLLFAFGIGSYLYNRKVYAPVPAVAS"
     gene            complement(6602..>6906)
                     /locus_tag="VGQ51_18350"
     CDS             complement(6602..>6906)
                     /locus_tag="VGQ51_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038607.1"
                     /translation="EIREWYGRQTSLKASVLYHHAVTKAMEFGWQFGGAIAGHIVGRF
                     PHEQPSDPQSLELDIHPDNDNDIFAPDANGDERHWILELQFIDRDREIGGYFEQLL"
BASE COUNT         1581 a         2268 c         1460 g         1597 t
ORIGIN      
        1 ccaccgatgt ctttaccagc ccggccggcc gcggcgtcaa accagccgcc ccgaacttat
       61 cggcctcata ccggtaacct ttttgttgct tcccttccgg caccgggttg gaccccgttg
      121 tcagcgccag cctgttgacc gcggctactt ggcttgccga cggaagattc agcacacatt
      181 cgcgtgacct taataaattt tccgtagtct tcgagaaggc agaaattccg atcatcgccc
      241 gccatcccaa ccagaagatg gatgaaatag gagcgagatt gtcggtaccg tcgccgttgc
      301 tggtgctgat cagcaccacc ggtgttccaa aataaaggat cgaaggatca attgtctttt
      361 tcatgaccca aagctcgcga ttgccgcgcg gcgggtcaac ccgattcttg cgatcttagc
      421 cgccgcatca ccgcctcgcc accacatccg ccgcatcacc ccgcccgcca atccgccgca
      481 tccgccgcac cgcgccctct tacatccccg aatccctcaa ccttacatac tcatccaacg
      541 acaaattctt cagccccttc tcctgcatcc tccggatgaa ctcgaccgtc accccatggt
      601 cctgaagctc cacggctttt tccagcgaca catccgcaaa cccgagccct tttatcccgc
      661 tgatgaacga cggctcgacc ccatgatcgc gtaattcctg cgccctgtcc gggctgacat
      721 tcttatatcc catcttctgg aattcccgga tgaactcgac cgtgacccca tgatcctgca
      781 gctcctgagc ccgctccagc gatagatccg tataccccag gtccctgaac cccttaatga
      841 actccaccgt cacaccatgg tcccggagct cgatcgcctc gtcgagcgag atatcgcgcg
      901 ctccggcctt tttcatatcg ctgatgaaat ccggggtgac cccatgatcc atcgcttcct
      961 gcgccttttc cagcgataca tgcgcataac cggcctcgtg caacgaattg ataaagtcgg
     1021 cgcctacgcc atgctcccgc aattcgacca tcttttccag cgacaccttg ccgtatcctt
     1081 cctttttaaa aagctcgaca taggcgctca tcacatgctg gttcatattc tgataggcaa
     1141 ggtccttcag ctgaccgatg gtgatcccct ggtaaccgtt ctccttcata taggcaaaat
     1201 aggccttatt gatattggta aaaaacaggt ggatcatcag cgcatccggt atctccctga
     1261 aaccctcgct ctccagatag ctcttgaatg ccgggtccgc gacaaataca taggtcccac
     1321 gacccgcgcc gttcgaccag ttgccggtga aggtcatcgt acccggatca cgttttaccg
     1381 tgacagtccc ggcagtgccg gtaggcagcg ttcccagctc gctgagatcg aaattggcgc
     1441 ccgagctcca gtggatcccg ccaaactgta tatgaactct gtctttatta atataggcgc
     1501 tccatacgcc cgggttgatc tcttcgttca tatcgtcctg cgcattggct atcagggaca
     1561 cgaagagggt gatcgccgtc aggcagacgg caacaagcat catttttttc ataacctttg
     1621 ttttaagatg taggacttat tccggcgtcc cttaatttga tataatcgtc aggttccagg
     1681 agcttcccga tcttcttttt ccacgaactg atgaacccga tggtcacacc gtgatcgacc
     1741 agctcgatgg ccttctcgag ggagatagcg gggaaaccca gaccatgcaa ttcccgtatg
     1801 aatccgaccg tcacgccatg atccttcaat tccagcgcca ggtccagcga tatcggagcg
     1861 gcatagcctt ccgcgataat gccggcaatg aaatccgtgc tcaccccatg atccgccagt
     1921 tcgatggcct tgtcgatcga aatatgttta tagcccattt tgtcgaagcc cgcgatgaat
     1981 tcggcggtca caccatgatc cctggcttct attaatttgt caatatcctc cttcggcact
     2041 gtatccttct gcgccatagc cttctgcttc gcttccttcg gcaccgtatc cacgattgcc
     2101 ttgtgtctcc tatccacatt cttcgcattg aaatgtctca ccaccgtcac cacctcgggc
     2161 tgcgctttgg caaccaccct agtttctgct accaccctcg tcttctccgt acgactatta
     2221 tgtatcgtga caaaagccgc actcaacacc atgatcccac ccatcaacac gatcctttcg
     2281 cccgcattca gcgagtgatt ggtcttttcg acgatccttt tcacgcgtct taccacctgg
     2341 ttctcttttg ctgcgaaccc gagcgcacaa ccggtcgtct gtccgtattc atgaaaggat
     2401 accagcgcct cgatcagcct gcgccggctc ccggtctcgc gaatggcaat atcgtcacaa
     2461 cagttttcgc gttcggcgcg gatcagcgat gatatccagc ggagcgcggg gttgaaaaag
     2521 aacatcgtat cgatcacatg ctgaataaaa ttgaacagat agtctttgcg tcggatatgc
     2581 gccagctcat gcagcaggat ggactccacc tggtccgcgg aaagatgcac cagcattccc
     2641 atgggaacca ggatcacggg cttcagcatg cccactacca cgggcacctt taccaacgcc
     2701 gattccagca gcgacaccgg tcgcgcgatc cctaattttt ctaaaagccg cccgagacgt
     2761 tgctgccact cttccggcgc cggctgattc ttatagtggc ggatccgctg ggcataaacc
     2821 cacccggata atagctgtac ccaccgggcc aggaatacga tcccccagat gaggaccacc
     2881 agcgcggcat gcgtgttgaa atagcccacc aacgcatcga tgcctgccgt caccccggaa
     2941 cgcgacacaa acgttgctcc cgcatccggc gccgccgtca ccccatgacc ccccttcacc
     3001 cccgcgtcca ccgtcaccct gcccgtttcc acgacaacac tcattcccag ctcacgataa
     3061 aaggtgtacc cgctgacgac gaggaaaagc aaaagccctc cacaaagcaa attgtaacgc
     3121 agcgcagcgt gcgatttcct cgtcaccacc aggacgaccc cggcgactcc cgcgaagagg
     3181 agtccctgcc acagcgagtg cagcagcgtc cagcacagcg cccgtattat cgtatccggt
     3241 ataaaatgca tatagtgtcg tttattgatc ctcgtccagc cggtccagca tcttctttat
     3301 catatccatg tccttctcat tcattttctc gtttcccagc aactccagca tcaaactctt
     3361 cgcgcttccc tcatacaccg aatccacaaa cttctttaag actatactct tcgtcttccc
     3421 ctcctccaaa agggcgctgt aaacatgcgt catgttgctt tcatcccgat gcaccagccc
     3481 tttttcgtgc attatctgca tcagcttgag cgtggacgta taactcagtg actggttctg
     3541 ctgcctgttc agctgatcgt tcacgaaccg gaccgtcgac ggcccatatt tccacaacac
     3601 ctgcaggatc tccaactgcg cccgggtagg ttctacattt tccatacacc aaagatataa
     3661 taaacaacgt acgaaaaagg ttatagcatc atgttaacat atatttaaca tttaggcggc
     3721 ccgcgtcacc gcctctgccc ccgtatttcg ctctggaatg ataaaatacc ttctgcccgc
     3781 tatcgcccct ttagccttca ccaaaggaga attctatgta cacatttgat cccggcgaag
     3841 gaatcgtcaa cgccgccgac atcaaacgcc tcctccgcaa ggaccgtctc ttcggtggca
     3901 tctataaaaa atatggcgcg cctcccccct ggtaccgcga gcccggcttc atctcgctgt
     3961 cgatgatcat cctcgaacaa caagtcagtc tcgcctccgc caaagcccat tttcaaaaac
     4021 tccacggcta tatcggcgaa ttcacgccct cgaacatcct ccgtctcacc gacgaagaaa
     4081 tgcgcacctg ccagatcagc cgccagaaag ccaaatatct ccgcgcactc tccgacgcga
     4141 tcaccggcaa cgccctcgat ctgcatggca tggccactct ccacgaatcg gcaatacggg
     4201 aaaagctcac gggcatcaaa ggcatcgggc gctggacttc cgacatctac ctgatgttct
     4261 gtctgcaggc cagggacgtc ttccccatcg gcgacatcgc gatcgtcaac accgtcaaag
     4321 agctcaccga cgcacgcaca caggaagaga tcctcaccct ctccgaaagc tggcgtcccc
     4381 ttcgctcgct cgccacctat tttctttggc ataattatct ttcaaaacgc ggtcgcagct
     4441 gatcgccgac gatccttcga ttcgcccaac attccttatc ttgccccacg ccgtcccaac
     4501 ccgcggcgcc gcccatgcaa caattcaccg tcagcgaagc cggttataag aagtacagca
     4561 ggaatagccg gcgcgtccta tacggattcg cgtccatcgt ggccgcgggg ctgctctatt
     4621 tactcgttct ggccattgaa aaaggtaaat tcgacatgac gatgatcatc ccaccctgtg
     4681 tcgtcgcggt tgccctgtct ttccagatcc cccgtatcct ccgccggcaa aaaaagatgg
     4741 tgatgagcta cacgatcaat ctgtcggacc acgagatcac ccgcgaacag gtcgacaccc
     4801 ggccgctgac catcaatttc atggagataa aagagatcac gaagacccga aaaggacatt
     4861 tcatgatcaa gggcctcacc cgcaccgatt atatcgttat ccccaattgg atcgacaatt
     4921 atccgcagct ggaaaaacaa ctccaaacgc tggcgcccat cgaagagaag accaaagaac
     4981 accgttatgc aacggttcgt gcgctgctga gcatgatctc gatacccttg atggtagggt
     5041 cctttctcgt caaagacaaa gccgtcgcag ccatcttcgc cgtacttgcc gtcggcctgc
     5101 tgggctggat atactatgaa acccgcgtca gtaagaatat gcccgagaac gtgcggcgca
     5161 ggagctggac ctatctcatc atgatcgccg ccatcattta tatcacctgg caaaaattta
     5221 cggcctagcg ccgccccctt caatagaaca acccataaac cctcccccgc gggatcttaa
     5281 acaccaccag aaacccatag tataaaaaaa gagagatagt cgtaatagga atatgtctca
     5341 gcgccgccag caaacccaac aggtacaaca ccccatctat cacaagtccc agcaccaacg
     5401 catcccgtag cttcagcacc ggcatgaacg ccgaatgcgt ccgatagctg acgatcagcg
     5461 gatccaggta tccaagcgcc agctgagcac tcgccttacc ctcaaaactg aaatcgtaat
     5521 gcttgacgca ctggacatat tcgcccagta tcatcaacgc ttcatccttc tcctgctcac
     5581 tttcgatctc atggaaatgc aggacgaaat ttcgcatcgt tctgtagtgt atgtattttc
     5641 tcattttggg gcaaagcatc gtttcccatt ccagcaccgc ctctttgatc tgttcgtagg
     5701 tcaaatgctc gcctccggca cctgtttcca ttgtcacgtt taggtttatt tccgcgatcg
     5761 gatcctgtgc gatcatccat gttccgcgca agcgcgccct gtgcgatcag ccatgttttc
     5821 gtgcaatcgg gcgctgaatt taccgcaatt ttggttccat aacaaaatct tccccgcggc
     5881 ccgcatgcgg ttagaacaaa tcctacacga aatacgtgga aaaaacggtt tttacacggc
     5941 ccgcccgctt cccccaaagt tccatctcac ttcccgcacg attcccgccc gctgcccgcg
     6001 cacctccttc cctccaccga cgccggaaaa ctgccgcgga ccaaaaaatc ttatatttga
     6061 ctataccaaa acaatgctct agctatgaaa aagaccagga tcatctattg ggtgctaacc
     6121 atcgtcttcg ccggcttcat ggccttcacc gccatccccg acgccctcgt tgtccccgac
     6181 gcagcaaaat tcatgaccca tctgggctat cccaactatt tcacgcattt catcggcatc
     6241 gccaaactcc tcggagccat cgccatcctg atcccaggtt tccgcacgat caaggaatgg
     6301 gcctacgccg gcctcttctt cgatctcatc ggcgcctttt attccaattt catggtcgat
     6361 ggcgccaatg cgccaatggt ggtcaccatg gtcctcctct tcgcattcgg catcggatca
     6421 tatctctaca accgcaaagt atacgcaccg gtcccagccg tcgcatcata agcacgtcgc
     6481 atcataagcc gtcgcatcta aagccgtcgc atccttactc cccgcatctc ccataaataa
     6541 aaagcccccc atcggggggc tccaatctac cgtgtgaccc tctgctaaac gccttgtttt
     6601 atcataatag ctgttcgaaa tatccaccta tctcccgatc cctgtctatg aattgaagct
     6661 caagtatcca atgtcgctca tccccgttcg catccggcgc aaaaatgtca ttgtcattgt
     6721 ccgggtgaat atccagctcc aggctctgcg ggtccgatgg ctgctcatgc gggaacctac
     6781 cgacgatatg ccccgcgatc gctcctccga attgccatcc gaattccatg gccttcgtca
     6841 cggcatggtg gtatagcacc gacgccttca gactcgtctg acgcccatac cattccctta
     6901 tttcat
//
LOCUS       DASYSV010000589        10987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1651241, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000589 DASYSV010000000
VERSION     DASYSV010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651241"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VGQ51_18355"
     CDS             <1..538
                     /locus_tag="VGQ51_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9038608.1"
                     /translation="AYGAEFLLRKQAGRLNGWVSYTWSRTFLKQDDPLAGQLINNGNW
                     YPASFDKPHNVNIVGNFRFTHRYSISTNVVYNTGRPITLPLAVFNLGGSPGLYYSQRN
                     QYRIPDYFRWDLSFTIDGNHRVKQVTHNFWTFGVYNLTARKNPYSIYFQQENGQIKGY
                     QLSIFGTAIPFVTYSIKF"
     gene            539..1708
                     /locus_tag="VGQ51_18360"
     CDS             539..1708
                     /locus_tag="VGQ51_18360"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEV9038609.1"
                     /translation="MTPNRISIVSSAILLITGSALLIAGLQACRQAYNPPAITASNSY
                     LVVDGFVNTGHASVSSFRLNRTRNLNDSTTIGVPELHAQFAILGKHGDAYPLADSAND
                     GTYFSAPLSLDTTQQYTISITTSDGRKYSSDGVSCKQSPPIDSLFWSQPSDLTIYAAT
                     HDPTGNTRYYRYDYIETWQHNSRLTSPWTVINNQVTPVDSTDETWYCWSTAHSTNVLI
                     TTSVALARDLVTAFPLNVIPKGDPRLAIGYSILVRQYALTEDAYNYWNLIEKTSQDVG
                     TLFDLQPTQLTGNIHCLTNPSEPIIGYITASSVTQQRLFIPDSVLNGWVDPVPPECQT
                     SMVPYPFSIFPAYPVTDTTVGPYYFISNGPMVVAPKSCLDCRYQGGTRIKPSFWP"
     gene            1724..2908
                     /locus_tag="VGQ51_18365"
     CDS             1724..2908
                     /locus_tag="VGQ51_18365"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEV9038610.1"
                     /translation="MHNALHRYKLLPLFPAVTLSLAGTIILLVVMGACRQSYNPPAIQ
                     ASNNYLVVDGFINTGRFALTTFNLNRTRSLNDSTTIGIPELHAQFSIIGKHGDVYPLT
                     DTGNTGFYSSGPLNLDTTQQYSIRITTADGRKYSSDAVPCKQSPPIDSIFWSQPGDLT
                     IYAATHDPTGNSRYYRYDYAETWQHNALLIAAWTLVNNQVAVADSSDENWYCWSSANS
                     TNVLITTSIALGQDVITAFPLITLPLGDPRVAVEYSILVRQYVLTPDAYDYWNLIEKT
                     SQSVGTLFDLQPTQLLGNIHCITNPSEPVIGYLSASSVTQQRIFIADTVLTAWVNPIS
                     LPGCRTSTFPYSFDFFPTYPVSDTSIAPYYFSGGTMILAPKFCLDCRYQGGTRIKPSF
                     WH"
     gene            2918..5488
                     /locus_tag="VGQ51_18370"
     CDS             2918..5488
                     /locus_tag="VGQ51_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038611.1"
                     /translation="MSNLFHRYRSFPLLLAAALSLAGFRSATAQSPDVHPITSQNPVA
                     DSLAAAFDRYPTRQERLFVHIDKTVYLAGEIIWFKAYDVDAGSNTSQSLSGSNTSRSL
                     SGSNTSQSLSSIAYVELLDKDQKSILQAKIELNNGTGNGSFRIPSSITSGHYTFRAYT
                     NWMKNFSPDFYFHQGLTIINTLTNEPLTDSARPAVATSSGSTQAAVLSTSAIRFFPEG
                     GNLVNGLPSVIAFKSLAPSCTGIILDQKKDTVARFTSQRFGMGHFSLTPLRGNTYIAL
                     VTTPAGTTTEKLPDAYDDGFVIHLEDIDARHLRITVRSTTTPADPTLYLFVHTRHRIR
                     SIQAHRLENNQTAFTIDKDSLDDGISHITLFTADRLPVCERLYCKPPQKRLHIDVSTP
                     AVTSSATASATQAAPRTKLTFDLSTTDPSGHPTPADLSMSVFLIDSLQPIPDENILSY
                     LLLRSDLKGKIESPASYFADTDPATTAALDDLMLTQGWSRFRWEDILRDKKPAFEFLP
                     EADGPIIHARLIDKATGQPPSPKIGYLSVPGRDFKLSTAISHNDGSLFFHVDRFYGNG
                     EIIVQTNMTTDSNYRIDVSNPFSDRFTAEADAGAPPAQSAIPPSPLQKDQLLNRSIAV
                     QAENAYRINEKNRLIPAAPADSLAFYGIADHRYKLDDYTRFVTMDEVIREFVENVKVR
                     QQSGKTIFRVRNALFNLFFEDDPLLLIDGVPVFHGDRIMALNPLKIEKIDVVSHRYYL
                     GPSISDGIISFRSYDGDLGGYQLDPGAVVVQYNGLQQHREFYTPVYATAAEIQSPLPD
                     LRNQLLWRPDVVTASTGRRELSVYTSDLTGTFAIVVQGLTADGLAGYKIKTFTVTRPQ
                     "
     gene            complement(5537..6304)
                     /locus_tag="VGQ51_18375"
     CDS             complement(5537..6304)
                     /locus_tag="VGQ51_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV9038612.1"
                     /translation="MSTLRERLKNVRLVRKLVYAIVGSISYPGLTIVNRLKISGTEIL
                     RKLPKKNVLFVSNHQTYFADVITFLHIFCAVKWRKENKLGLPYYLLNPFTNVYYVAAE
                     ETMRSSWVSRLFTMAGALTVKRTWNKTSHEVRRGLDPSDTRKITRALENSWVITFPQG
                     TTKAFAPGRKGTALIIKQTRPIVVPVVISGFWRAFDKKGLKFKKRGTQLSVTFKEPLD
                     IDYDAPTERILEQVMDAIEQSKSFMMKGAHHWLKVPV"
     gene            6446..7102
                     /gene="hxpB"
                     /locus_tag="VGQ51_18380"
     CDS             6446..7102
                     /gene="hxpB"
                     /locus_tag="VGQ51_18380"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HEV9038613.1"
                     /translation="MRLNTVIFDMDGLLIDSEPFWQEAGMETLLQFNVTLDPVQYHHT
                     TGLRTREWLDHWFTHFAIGKERIPAAEAALHHNVIEKIKTHADPLPGVHEILAFFRSR
                     NFRIGLATSSPLSLVKVVVEKLGIENNFQAVASAEHLPYGKPHPQVYMDCAQSLAVSP
                     LQCLAFEDSFNGVIAAKAARMTCVAIPVPDQAHLPKWGAADLRLGSLLDFTDHELSSL
                     "
     gene            complement(7153..9096)
                     /gene="rpsA"
                     /locus_tag="VGQ51_18385"
     CDS             complement(7153..9096)
                     /gene="rpsA"
                     /locus_tag="VGQ51_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV9038614.1"
                     /translation="MSENNIVNSNAESQETSEVATATAEATATPKAPVPVQTAHDDFD
                     WSIDKRNVAAYTKEEKEKYDKVYENTFISITDGELVKGLVVGLTKTDVVVNIGFKSDG
                     LVSLNEFRDLANLKTGDEVEVMVVEKEDREGHLHLSRKQARITRAWEKIVEVHKSGEV
                     VTGTVTSKTKGGLIVDVFGMETFLPGSQIDVKPVTDYDQFVGKTMEFKVVKINEAIKN
                     AVVSHKALIESDIEAQRAEIISKLEKGQVLEGTIKNITDFGAFMDLGGLDGLLYITDI
                     SWGRISHPSEVLKLDQKLQVVVLDFDDGKKRISLGLKQLTPHPWDVLPPTIKEGEIVK
                     GRVVNIEDYGAFLEIMPGVEGLVHVSEITWANTPINAKEFFKLGDEHNAKVVTLDKDA
                     RKMSLSIKQLSEDPWNTIQDKFPVGSRHNGLVKNITPYGVFVELAPGIGGMIHISDLS
                     WLKRFNHPSEYTKVGQHIDVMILGIDKDNRKLQLGHKQLEEDPWNALEETFNVGSVHE
                     GLVIRKDEKGAIVQLPYGLEGFAPNRHLNKEDGKQVQADETAPFLVIEFDRNEKRIVL
                     SHTRLWEQVKHEEKQAAQKEARADADKTRKAMKNIQQKVEKPTLGDLGALASIKEKLK
                     QEEKDKDQHAGGNAGAAGTEEKK"
     gene            complement(9162..9596)
                     /locus_tag="VGQ51_18390"
     CDS             complement(9162..9596)
                     /locus_tag="VGQ51_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV9038615.1"
                     /translation="MGLNSFAPVLTIKVLAPAVEFYKKAFGAEELLIVKNDDGSVHVA
                     ELTFQGMIFHLHQEMPEPGKGRAPETAGGTTVELGVFLEDPDALMDSALAAGARLIHP
                     MKDYEYGYRQGSFADPFGHHWTLQKKLANAWTGVIGGDGKPE"
     gene            complement(9586..10812)
                     /locus_tag="VGQ51_18395"
     CDS             complement(9586..10812)
                     /locus_tag="VGQ51_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV9038616.1"
                     /translation="MTLIVIGGGAAGIFCAVNAARLRPGLRVIVLEKTGKLLSKVKVS
                     GGGRCNVTHACFDIADMAKKYPRGGHFVRKAFHRWFTTDTIEWFASRGVELKTEADGR
                     MFPVTDSSQTVIGCLLREAERLGVEIWMHAEVRGVQPANPGFSVRLADGREILGDYVC
                     IACGGYPKAPMFDWIRALGHSIEDPVPSLFTFNMPGDAITQLRGVSVGEAQVKIGRLV
                     ERGPVLITHWGLSGPAVLRLSAWGARELAGLGYNFSIVVNWVPELNELELRERLQELR
                     YEWGAKMLGSVVPFAGIPRRLWEYWLDSSGIRKDIRWADLAARDQNRLAGLLAAGNFI
                     INGKTTFKEEFVTAGGVRLAEVDPATCQSRKVAGLFFAGEILDVDGVTGGFNFQHAWT
                     SGWVVADTIKNLSYGT"
BASE COUNT         2401 a         3773 c         2317 g         2496 t
ORIGIN      
        1 agcctatggc gcggaattcc tcctcaggaa acaggccggc cggctcaacg ggtgggtcag
       61 ctacacctgg tcgcgcacct tcctcaaaca ggacgaccct ctcgccggtc agctcatcaa
      121 caatggcaac tggtacccgg ccagtttcga taagccacat aacgtcaata ttgtcggtaa
      181 tttccgattc acccatcgct atagcatctc cacgaatgtc gtatataaca ccggcagacc
      241 catcaccctc cccctggcgg tctttaacct cggcggctct cccggtctct attattcgca
      301 acgcaaccaa taccggatac ccgattattt cagatgggac ctgtccttca ccatcgacgg
      361 taaccaccgg gtaaaacaag tcacccataa tttctggacc ttcggcgtat acaacctgac
      421 agcacgcaaa aatccttatt ctatttattt ccagcaggag aacgggcaga tcaaggggta
      481 tcagttatcc attttcggta cggccatccc attcgtaacc tattctatca aattttagat
      541 gacgcccaat cgcatatcca tcgtctcttc cgccatcctg ctgatcacgg gctccgcgct
      601 gctgattgcc ggtctgcaag cctgcagaca agcctataac ccgccggcta tcacggcgtc
      661 caacagctat cttgtcgtcg atggcttcgt caacaccggc catgcatccg tctcctcatt
      721 ccgtctgaac aggacacgca acctcaatga ttccaccacg atcggcgtcc ccgagctgca
      781 cgcgcagttc gccatcctcg gaaaacacgg agacgcctac ccgctggcgg attccgcgaa
      841 tgacggcacc tacttcagcg ccccgctgtc cctggacacc acgcagcagt atactatctc
      901 gatcacgaca tccgacggcc gcaaatattc ctcggatgga gtttcctgca aacagtctcc
      961 gccgatcgac agccttttct ggagccagcc gtccgatctc accatctatg ccgctaccca
     1021 tgaccccacc ggcaataccc gctattaccg gtacgactat atcgaaacct ggcagcacaa
     1081 ttccaggctg accagcccct ggacagtcat caataaccag gtcactcccg tggattccac
     1141 cgacgaaaca tggtattgct ggtcgaccgc acattcgacc aatgtcctga tcactacttc
     1201 cgtcgcgctt gcccgggatc ttgtcaccgc cttcccctta aacgtaatcc cgaagggcga
     1261 tccccggctc gctatcgggt acagcatcct ggtgcgacaa tacgcgctca ccgaagacgc
     1321 atacaattat tggaacctca tcgaaaaaac atcccaggac gtcggaaccc tattcgatct
     1381 gcaacccacg cagctcaccg gcaatatcca ctgcctcacc aacccttcgg aaccgatcat
     1441 cggctatatc acggcatctt ccgtgacgca gcagcgctta ttcatccccg attccgtgct
     1501 caacggttgg gtggatcccg tcccgccgga atgccaaaca tccatggtcc cctacccttt
     1561 ttcgatcttc ccggcttatc cggtaaccga taccactgtc ggcccctatt attttatctc
     1621 caatggcccc atggtagtag ccccaaaatc ctgcctggac tgccgctatc agggcggcac
     1681 ccggatcaaa ccctcattct ggccataatg caaaccaccg ctaatgcata acgcactcca
     1741 tagatacaaa ctcctcccgc tgttccccgc cgtcacactc tccctcgccg gcaccatcat
     1801 actgttggtc gtcatggggg cctgcagaca gtcctataac ccgcccgcca tacaggcgtc
     1861 caacaattac ctggtcgtcg atggctttat caataccggc cggttcgccc tcacgacgtt
     1921 caatctcaac cggacccgga gcctcaacga ctccacgact atcggcattc ccgaactgca
     1981 tgcgcaattc tccattattg gaaaacacgg cgatgtatat ccgctgaccg atacggggaa
     2041 cacgggcttc tattccagcg ggccgctgaa cctcgacacc acccaacagt atagcattag
     2101 gatcactacc gcggacggcc ggaaatactc gtcggatgcg gtaccctgta aacaatcccc
     2161 gccgatcgac agcatcttct ggagccagcc cggcgatctc accatctacg ccgccacaca
     2221 cgaccccacc ggaaatagcc ggtattacag gtatgattat gccgaaacct ggcagcacaa
     2281 cgccctgctg attgctgctt ggacgctggt caacaatcag gtcgccgtcg ccgattccag
     2341 tgacgagaac tggtattgct ggtcctccgc aaactcgacc aatgtattga tcactacctc
     2401 catcgcactt ggccaggacg tcatcaccgc ctttccgctg atcactttgc ctctcggcga
     2461 ccccagggtc gcggtcgaat acagcatcct ggtcagacaa tacgtgctca ccccggatgc
     2521 ctacgattat tggaacctca tcgaaaagac atcgcagagc gtcgggaccc tcttcgatct
     2581 gcaacccacg caactcctcg gaaatatcca ttgtatcacc aatccgtccg aacccgtcat
     2641 cggctatctc agcgcgtcat ccgtaacgca gcaacgtatt ttcatcgccg ataccgttct
     2701 cacggcttgg gtgaatccca tatccctccc cggatgccgt acctcaacgt tcccttacag
     2761 ctttgacttt ttcccgacct accccgtatc cgacacttcg atcgcaccct attatttcag
     2821 cggcggcact atgatcctgg cgccgaaatt ctgtctggat tgccggtatc agggaggcac
     2881 ccggatcaaa ccttctttct ggcactaata caaaaaaatg agtaacttat ttcatcgata
     2941 caggtccttc ccgctgctac ttgccgccgc tctttcgctt gccggcttcc gctccgcgac
     3001 ggcacaaagc ccggacgttc accccatcac gtcccaaaac cccgtcgctg atagtctcgc
     3061 cgcggctttc gatcgctatc caacccgcca ggagcgactc ttcgtacata tagataagac
     3121 ggtgtatctc gccggcgaga tcatctggtt taaagcttac gatgtcgacg ccggctccaa
     3181 tacctcccaa tccctttccg gctccaatac ctcccggtcc ctttccggct ccaatacctc
     3241 ccaatccctc tccagcatcg cctatgtgga gctcctggat aaagaccaaa aatccatcct
     3301 ccaggccaag atcgaattga acaacggcac cggcaacggc tccttccgca taccatcctc
     3361 cattacttcc ggccattata cttttcgcgc ctacaccaac tggatgaaga acttcagccc
     3421 ggatttttat ttccaccagg ggctcacgat catcaacacg ctcacgaacg aacctttaac
     3481 cgactccgca cggcccgctg tcgccacctc gtccgggtcc acacaggccg ccgtcctgtc
     3541 aacctccgcc atccgcttct tccccgaagg cggcaatctg gtcaacggcc tgccctccgt
     3601 catagctttc aaatccctcg ccccctcctg caccggcatc atactcgatc agaaaaaaga
     3661 taccgtcgcc cgattcacct cccagcgctt tggcatggga catttcagcc ttactcctct
     3721 ccgcggcaac acctatatcg ctttggtcac aacccccgcc ggcaccacga ccgaaaaact
     3781 ccctgacgct tatgacgacg gtttcgtcat acacctcgaa gatatcgacg cccgccacct
     3841 gcgcatcacc gttcgctcga caacgacacc cgccgatccg accctctatc ttttcgtcca
     3901 tacccgccat cgcatcaggt ccattcaggc acaccgcctc gagaacaacc aaaccgcatt
     3961 caccatcgac aaagacagcc tcgacgacgg catctcgcat atcacccttt ttacggcgga
     4021 ccgcctgcca gtctgcgaac gcctctattg caaaccgcct caaaagcgat tacacatcga
     4081 cgtgagcacc cccgccgtca cctcatccgc caccgcctcc gctacccaag ccgccccccg
     4141 caccaaacta actttcgatc tctccactac cgacccctcc ggccacccta ccccggccga
     4201 tctctcgatg tccgtctttc tcatcgactc gctccagccc attcccgacg aaaacatcct
     4261 cagttatctg ctactgcgct cggatctcaa aggcaaaata gaatcccccg cctcatattt
     4321 cgcagatacc gaccccgcta cgaccgccgc cctcgacgat ctcatgctca ctcagggctg
     4381 gagccgcttc cgctgggaag acatcctccg cgacaaaaaa ccggctttcg aatttctgcc
     4441 ggaagcggac ggccccatca ttcatgcaag gctcatcgac aaagcgaccg gccagccccc
     4501 ctcgccgaag atcggctacc tgtcggtgcc cggccgcgat ttcaaactct ccactgccat
     4561 cagccacaac gacggaagtc ttttcttcca cgtggatcga ttctatggta acggcgagat
     4621 catcgtccag accaatatga cgaccgatag caattaccgg atcgacgtct ccaacccttt
     4681 ctccgaccgt ttcaccgccg aggccgacgc cggggcgcca cccgcccaat ccgcgatccc
     4741 gccgtcccca cttcaaaaag atcaattgct caaccgcagc atcgctgtcc aggccgaaaa
     4801 tgcctatcgt atcaacgaaa agaatcgcct catccctgca gcgccggcgg actcgcttgc
     4861 cttctatggc attgcggatc atcgttataa gttggacgac tatacccgtt tcgtgacaat
     4921 ggatgaagtg attcgcgaat tcgtggagaa tgtaaaagtg cggcagcaat ccggcaagac
     4981 tattttcagg gtccggaacg ccctgttcaa tctcttcttc gaggacgacc cattattgct
     5041 catcgacggc gtccccgtgt tccatggcga caggataatg gccctcaacc cgctcaagat
     5101 cgaaaagatc gacgtcgtct cgcatcgcta ttatctcgga ccttccatca gcgacggcat
     5161 tatcagcttc aggtcctatg acggcgatct gggcggttat cagctcgatc cgggcgccgt
     5221 cgtggtacaa tacaatgggc tgcagcaaca tcgtgaattc tatactccgg tctatgcgac
     5281 agccgcggag atccagagtc ccttacccga tctcaggaac cagctgctgt ggcgcccaga
     5341 cgtcgtaacg gcctccaccg gcaggcgtga actgtcagtc tatacttccg atctgacggg
     5401 cacattcgcc atcgtcgtgc agggtctcac agccgacggc cttgcaggct ataaaataaa
     5461 aacattcacg gtaacccgcc ctcagtgaac caaggcctca cgagaggtcc ccggctccca
     5521 atgatccttc gcgatctcac accggaacct tcaaccagtg atgcgccccc ttcatcataa
     5581 atgatttact ctgctcgatc gcatccatca cctgctccaa tatccgttcc gtcggcgcgt
     5641 cataatcgat atccagcggc tctttgaaag tcaccgataa ttgtgtcccg cgtttcttga
     5701 atttcaaccc cttcttgtcg aaggcgcgcc aaaacccgct gatcaccacc gggacgacga
     5761 tcggccgggt ctgtttaatg atcagtgccg ttccttttct tccgggcgcg aatgccttcg
     5821 tcgttccctg cgggaaagtg ataacccagc tattctccag cgcccgcgtt atcttccgcg
     5881 tatccgaagg atccaacccg cgcctgacct catgacttgt cttgttccag gtccgtttca
     5941 ccgtcaatgc gcctgccatg gtgaacaacc tgctcaccca ggatgaccgc atcgtctcct
     6001 cagccgccac atagtacacg tttgtaaacg gattaagcaa atagtacggc aaccccaatt
     6061 tgttctcctt cctccacttc acggcgcaaa agatgtgaag gaaggtgatc acatcggcaa
     6121 aataggtctg atgattgctg acgaaaagca cattcttctt cggcagctta cgcaggatct
     6181 ccgtaccgct gatcttcagc cggttcacaa tcgtcagacc gggataggaa atgctcccca
     6241 cgatggcata aaccagtttc ctcaccagcc tgacattctt caatcgttct ctcagtgtgc
     6301 tcatacccca atttacgaat agttgcggga actcaccgcc ccaactttcc tccgtctcca
     6361 cggctctcct ctctcgcgcc atctcaggcg ccgccccaac tttcctctgc cttcgcatcc
     6421 cgttatgcgc taactttgtc tccctatgcg tctcaatacc gttatcttcg acatggacgg
     6481 actgctgatc gattccgaac ccttctggca ggaagccggc atggaaaccc tgcttcagtt
     6541 caacgtcacg ctcgatcccg ttcaatatca tcacaccacc ggcctgagga cgagggaatg
     6601 gctcgatcac tggttcactc acttcgccat cggcaaggaa cgtatccccg ccgccgaagc
     6661 cgcgctgcat cacaatgtca tagaaaagat caaaacacat gccgatcccc ttcccggcgt
     6721 tcacgagata ctcgccttct tccggtcgcg gaattttcgc atcgggctcg ctacttcctc
     6781 ccctctctcc ctcgtcaagg tcgtcgtcga aaaactaggc atcgaaaata attttcaggc
     6841 ggtagcgtcc gccgaacacc ttccctacgg caaaccacat ccacaagtct acatggactg
     6901 cgcccaatcc ctcgccgtaa gcccactcca atgtcttgca ttcgaagatt ccttcaacgg
     6961 cgtcatcgcc gccaaagccg cccgaatgac ctgcgtcgcc attcccgttc ccgatcaggc
     7021 ccacctcccc aaatggggcg cagccgacct caggctcggc tcgctcctcg acttcactga
     7081 tcatgagctt tcctcattat aaaaaaaccc cggcagccgc cggggtttct attttgctat
     7141 cttcttggag tcttatttct tctcttcggt accggcagca ccggcgttcc caccagcatg
     7201 ctgatcctta tccttctcct cctgtttcag cttctcctta attgacgcca gcgcaccaag
     7261 atcacccagc gtcggcttct ccaccttttg ctggatattc ttcatcgcct tgcgcgtctt
     7321 gtcagcatcg gcccttgctt ccttctgagc agcttgcttc tcctcatgct tgacctgttc
     7381 ccacagacgg gtatgcgaca gcacgatcct cttctcgttc cgatcgaact cgatcaccag
     7441 gaagggagcg gtttcatcgg cctgcacctg cttgccatct tctttgttga gatgacggtt
     7501 gggagcaaaa ccttcgagac cgtaaggcag ctgcacgatg gcgcccttct catccttgcg
     7561 gatgacgaga ccttcatgca cggagcccac gttgaacgtc tcttccagcg cattccaggg
     7621 atcttcctcc agctgtttgt gtccaagttg caacttgcgg ttgtccttgt cgatacccag
     7681 gatcatgacg tcgatatgct gtcccacctt cgtgtactcg gaggggtgat tgaaccgttt
     7741 aagccagctc aggtcgctga tgtggatcat gccgccgatc cctggcgcca gttccacaaa
     7801 aactccataa ggcgtgatgt tcttcaccag cccattgtgc ctgcttccaa ccgggaattt
     7861 gtcctgtatc gtattccatg ggtcttcgct cagttgcttg atcgaaagac tcatcttgcg
     7921 cgcatccttg tccagcgtaa cgaccttggc attgtgctcg tcgcccaatt tgaagaattc
     7981 cttcgcgttg atcggcgtgt tggcccaggt gatctcgctg acgtgtacca gaccttccac
     8041 gcccggcatg atctccagga atgcgccgta gtcttcgata ttgacgacac ggcccttgac
     8101 gatctcgcct tctttgatag tcggaggcaa tacatcccaa ggatgcggcg tcagctgttt
     8161 caaacccagg ctgatacgct tcttgccgtc gtcgaaatcg agcaccacaa cctgcaactt
     8221 ctgatcgagc ttcagcactt cgctcggatg gctgatacgt ccccagctga tatcggtgat
     8281 atacagcaga ccgtccagac cacccaggtc catgaatgca ccgaaatcgg tgatattctt
     8341 gatcgttcct tccagcacct gtcctttctc caacttgctg atgatctccg cacgttgtgc
     8401 ttcgatatcg ctttcgataa gggccttgtg cgatacgacc gcattcttaa tggcctcgtt
     8461 gatcttcaca actttaaatt ccatcgtctt gccgacaaac tggtcgtagt cggtaacagg
     8521 cttcacgtcg atctgcgatc cgggcaggaa ggtctccatt ccgaatacat cgacgatcag
     8581 gccgcccttg gtcttgctgg taacggtacc ggtaacgact tcgccggatt tgtgtacctc
     8641 gacgatcttt tcccacgcgc gcgtgatcct ggcctgtttg cggctcagat gcaaatgacc
     8701 ttcccggtct tccttttcga ccaccatcac ttccacctcg tcaccggtct tcagattcgc
     8761 caggtcgcgg aattcattca gggataccag accatcgctc ttgaaaccga tattgacgac
     8821 aacatcggtc tttgtcaatc cgacgacaag acccttgacc aattccccat ccgtgatcga
     8881 aataaaggta ttctcataca ctttatcgta cttttccttc tcctccttcg tataggctgc
     8941 gacgtttctc ttgtcgatac tccagtcgaa atcgtcatgc gcagtctgca cgggcacagg
     9001 tgcttttggt gtcgctgtag cctcggccgt agcagtcgcc acttccgaag tctcctgtga
     9061 ctcagcgttt gagttaacaa tattgttttc agacataata aaaccccgag ttttttgatc
     9121 ggggctgcaa agatacgaaa ttcccattga ttttcagcca ctcactccgg cttaccatcc
     9181 cctcctatca cccccgtcca cgcattcgcc agcttcttct gcaacgtcca atgatgcccg
     9241 aacggatcgg caaacgatcc ctgccgatac ccatattcat aatccttcat cggatggatc
     9301 aaccgcgcgc ccgctgctaa cgcactgtcc atcaatgcgt ccggatcctc caaaaacacc
     9361 cccagttcca ccgtcgttcc ccctgccgtc tccggcgccc gcccttttcc cggctccggc
     9421 atctcctggt gcagatgaaa gatcattccc tggaacgtca gctcggccac atggaccgat
     9481 ccgtcatcgt tcttgacgat cagcagctcc tccgccccga acgctttctt ataaaattcc
     9541 accgccggcg ccagcacctt tatggtcaac accggcgcaa atgaattaag tcccatatga
     9601 taggtttttg attgtatcgg ccacgaccca tccgctcgtc cacgcatgct gaaaattgaa
     9661 gcctcccgtc accccatcca catcgagtat ctctcccgca aagaacagcc ctgccacctt
     9721 ccggctctga caggtggccg gatccacctc cgccaaccgt accccgcccg ccgtcacgaa
     9781 ctcttccttg aatgtcgttt ttccatttat tataaagtta cccgcggcaa gcaagccagc
     9841 caaacgattt tgatcccggg cggccaaatc cgcccaccgg atgtcctttc gaatgcccga
     9901 actgtccagc caatactccc acaaccgccg cgggataccc gcgaagggca ccaccgaccc
     9961 gagcattttt gcgccccact cataacgcaa ctcctgcaaa cgctccctca gctccagctc
    10021 attcagctcc ggcacccagt tgacgacgat cgaaaagtta taccccaatc cggccagttc
    10081 tctcgccccc catgccgaca accgcaatac ggccggcccg ctaagtcccc aatgtgtgat
    10141 cagcaccggc ccccgttcca cgagcctgcc gatcttcacc tgtgcctcgc cgacagaaac
    10201 acccctcagc tgggtgatcg catctcccgg catattgaag gtgaagagcg atggcacggg
    10261 gtcctcgata ctatgcccca acgcccgtat ccaatcgaac atcggagcct tcggatatcc
    10321 tccgcaagcg atacacacat aatcgcccaa aatctccctt ccatccgcca accgcaccga
    10381 gaaccccgga ttcgccggct gcacgccgcg aacttccgca tgcatccaaa tctccacacc
    10441 aagccgctcc gcctccctta acaaacagcc gatcaccgtc tgcgacgagt ccgtcaccgg
    10501 gaacatcctc ccatcggcct ctgtcttcaa ctccactcca cgtgatgcaa accactcgat
    10561 cgtatccgtc gtaaaccacc ggtgaaaagc cttccgtaca aaatgtcctc cacgcgggta
    10621 tttcttcgcc atatccgcga tatcgaaaca ggcatgcgta acattacacc ggccaccccc
    10681 gctgaccttc actttggaaa gcagcttacc ggtcttctcc agcacaatga cccggagccc
    10741 cggcctgaga cgcgccgcat tcacggcaca aaaaatgccc gcggccccgc cgccgatcac
    10801 aataagtgtc ataacttaag actgcagcgc agctgcatgt cctgtaaata gatgaggatt
    10861 ggccgcctcg gcagcctcta taatgctata gtcggacagg atatccgcct tgcctctttt
    10921 aaccgctata tcgtgctcga aatgaaccga tggctggtga tccgatgtgc gcaccgtcca
    10981 accgtca
//
LOCUS       DASYSV010000590         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2041173, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000590 DASYSV010000000
VERSION     DASYSV010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041173"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VGQ51_18400"
     CDS             <1..671
                     /locus_tag="VGQ51_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEV9038617.1"
                     /translation="ESALLQYGGECDKMRLDYAFRQLMSWYKGDGAYGDGPEFHWDYY
                     NSFVMQPMLLEASQVLQKADPKNADNKFDTAIFRRSQRYAVVLERMISPEGTYPPIGR
                     SLAYRFGAFHLLAKMALMKALPNTLAPQQVRAALYAVIHRQLEAPGTFDKDGWLQIGL
                     YGHQPGIGESYISTGSLYLCSEAFLPLGLPATDIFWQGEDLPWTALRIWQGENITTDH
                     ALQP"
     gene            655..2148
                     /locus_tag="VGQ51_18405"
     CDS             655..2148
                     /locus_tag="VGQ51_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEV9038618.1"
                     /translation="MRYNHKLKCLLAGALMALAFGQLYAQRPAQPSQRPSTARTRPNV
                     IFILMDDMGYGDIEPYGMTGIPTPNFDRLAAEGMRFTHYNAAQPICTASRAALLTGCY
                     SNRVGMHGALGPLANEALDPKEETVALLLKRAGYRTAMIGKWHLGRKPPFFPIHYGFD
                     SFYGLPYSNDMWPRDIDGNRITDTSNARSRWPELVMYEGDKATDTINTYAKQEELTTR
                     YTERAVSFIKANRNRPFFLYLAHSMTHVPLAVSDKFRGKSQLGLFGDVIMEVDWSLGQ
                     IMKTLDETGLARNTLLIITSDNGPWLNYGNHAGSSGGFREGKATTFEGGTRVPLLVRW
                     PARIKGGSINSHLMTNMDWLPTIAAATGAPLPKQRIDGLNFLPALTDPAKSPREVFYY
                     YFGVNELQAVRYRNWKLVLPHTTSSYRLLHGKNGAGGKIAHVAVPIALYDLAHDPGEA
                     YDVQELYPEIKEKLLKLADSARDDLGDDLTGKQGANRRPPAKIAPDR"
     gene            2218..>2909
                     /locus_tag="VGQ51_18410"
     CDS             2218..>2909
                     /locus_tag="VGQ51_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV9038619.1"
                     /translation="MNQYDAIVIGSGISGGWAAKELCDHGMNTLLLERGRPVRHLKDY
                     PTAMKAPWEFPHRGELPLAVTDENPVVSRCYAYGEATQQFFVPDKAHPYIQEKPFDWI
                     RGYQVGGKSLLWARQTQRWSPFEFEGPARDGFAVPWPIGYADLAPWYSHVERFAGISG
                     NKDGLAQLPDGEFLPPWEMNCVEKHISDKITERFPDRPVIIGRCAHLTQPQPVHLQQG
                     RGQCQARNLCERG"
BASE COUNT          648 a          852 c          814 g          595 t
ORIGIN      
        1 tcgaatccgc gcttctccaa tatgggggcg agtgcgacaa gatgcggctg gattatgcct
       61 ttcgccagct gatgagttgg tacaagggcg acggcgccta tggagacggt cccgaattcc
      121 attgggacta ctacaacagc ttcgtcatgc agcctatgct gctggaggct tcacaggtgc
      181 tgcaaaaagc cgatccgaag aacgccgaca ataaattcga taccgcaatc ttccggcgga
      241 gccagcgcta tgccgtggtg ctcgagcgga tgatctctcc cgaaggcacc tatccgccca
      301 tcggccgcag tctggcctac cgattcggcg ctttccatct gctggcgaag atggcactga
      361 tgaaagcgtt gccgaacacg cttgccccac aacaggtacg tgcagcgctg tatgccgtta
      421 tccaccggca gctggaggcg ccgggcacct tcgacaaaga cggctggctt cagatcggtc
      481 tgtatggcca tcaacccggc atcggcgaga gctatatttc gacaggcagc ctgtatcttt
      541 gctccgaagc ctttctgccg ctgggcttgc ccgccaccga tatattctgg cagggagaag
      601 accttccctg gacggcgctc aggatctggc agggagagaa cataactacc gatcatgcgt
      661 tacaaccata aactaaagtg ccttctggca ggcgccctca tggcccttgc tttcgggcag
      721 ctatatgcgc agcggccagc ccagccatcg caacgacctt cgacggcccg cacccgcccc
      781 aatgtcatct tcattttgat ggacgatatg ggctacggcg acatcgaacc ttacgggatg
      841 acaggtatcc ctacacctaa tttcgaccgg ttggctgccg aaggcatgcg tttcacccat
      901 tacaatgcgg ctcaacccat ctgcaccgca tcccgggcag cgctgctcac cggctgctat
      961 tccaacaggg tcgggatgca cggcgccctg ggccccctcg ccaacgaagc gctcgatccg
     1021 aaagaagaga ccgtggcgct cctcctgaaa agggccggct atcgcacggc gatgatcggc
     1081 aaatggcatc tcggccgcaa acctcctttc tttcctatcc actacggttt cgattccttt
     1141 tatggcctac cctattcgaa tgatatgtgg ccgcgggata tcgacggcaa ccggatcacc
     1201 gatacgtcga atgcacgctc ccgttggcct gagctggtta tgtacgaggg cgataaggcc
     1261 accgacacga tcaacaccta tgcgaagcag gaagagctga cgacacggta tacagaacgg
     1321 gccgtatctt ttattaaagc caaccgtaac aggcctttct ttttatatct cgcccactcc
     1381 atgacgcacg tgccgcttgc ggtgtccgat aagttcaggg gaaagagcca gcttggtctc
     1441 tttggcgacg tcatcatgga ggtggactgg agcctcggac agatcatgaa gacactggat
     1501 gaaacgggtc tcgcccgtaa cacgctgctc attatcacca gcgacaacgg tccgtggctc
     1561 aactatggca atcatgcggg gagctcgggc gggttccgcg aaggcaaggc caccaccttc
     1621 gaaggaggga cccgcgtccc gctgctggtc cgctggccgg ccaggatcaa aggcggcagc
     1681 atcaacagcc acctgatgac gaatatggac tggttgccga cgatcgccgc ggccacgggc
     1741 gctcccttgc ccaaacaacg catcgacgga cttaacttcc tgccggccct taccgatcct
     1801 gcaaaaagcc cgcgggaagt tttctactat tactttgggg tgaacgagct tcaggccgtc
     1861 cgctatagga attggaagct ggtgttgccg catactacat cgagctacag acttcttcat
     1921 ggtaagaatg gcgctggcgg caagatcgca catgtagccg tgccgatagc cttgtatgac
     1981 ctggcgcacg atcccgggga agcgtatgac gtacaggaac tgtatccgga gataaaggaa
     2041 aaattattga agctggcgga ctcggcgcgc gacgacctcg gagatgatct gacaggaaaa
     2101 cagggtgcta acagacgacc gccggcaaag atcgctcccg atcggtgatc tcatttttct
     2161 ctaaatttat tgaattgccg gtggtttcac cgaacttacc gtaccaatgt ttaccaaatg
     2221 aaccaatacg acgccatcgt catcggcagc ggcatcagcg ggggctgggc tgcaaaagaa
     2281 ctttgcgatc atggtatgaa tacccttctt ctggaacgcg ggcggcccgt ccggcacctc
     2341 aaggattacc cgacggctat gaaggctccc tgggaatttc cgcatcgggg tgaactgccg
     2401 ctggcggtca ccgatgagaa ccccgtcgtc agccgttgtt atgcctatgg cgaggcgact
     2461 caacaatttt ttgtaccgga caaagcgcat ccttatatac aggaaaagcc cttcgattgg
     2521 atacgcggtt accaggtagg cggcaaatcg ctcttatggg cccggcagac ccagcgttgg
     2581 agtcccttcg agttcgaggg cccggcgcgg gacggttttg cggtaccgtg gcccatcggg
     2641 tatgcagacc tggcgccctg gtatagtcat gtggagcggt ttgcagggat cagcggtaat
     2701 aaagacggtc tcgcccagtt gccggatggc gagttcctgc cgccctggga aatgaactgc
     2761 gtagaaaaac atataagcga caagatcacc gaaagatttc cggaccgtcc cgttattatc
     2821 ggacgctgcg cgcatctcac ccagccgcaa cccgtccatc tccagcaggg caggggtcag
     2881 tgccaggccc gcaatctatg cgaacgagg
//
LOCUS       DASYSV010000591         4150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1917167, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000591 DASYSV010000000
VERSION     DASYSV010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917167"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1801)
                     /locus_tag="VGQ51_18415"
     CDS             complement(<1..1801)
                     /locus_tag="VGQ51_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEV9038620.1"
                     /translation="MLRRPLICVFLLTICCCLLNSITAQAQVTDTTKNAHITDSLKAQ
                     TDTTHPVSIDPELVMLASGKTPPREYTIAGIKVSGTRYLDESLLTSISGLTVGDKVTI
                     PGGDNFSKAIQNLWKQHLFANISIYFTKLAGTSLYVEINVTERPRLSNFYFKGSGVNK
                     GASDDLATKTGLIKGRVITEDMKRSAIQAIKKYYADKGYQDARIRTTETRDPAVQNSE
                     VLTFLIDRGPKVRIYQINFFGNDAIREAKLKKQLKDTKELSRISLYPPSNITPYGPHD
                     SVTLKQYLNHWGFLSFTSTKEFIDPYFRFKMFSSAKFNASKFEDDKEKVVTYYNSQGY
                     RDAQVVADTNYVDTSRQGKGHLMIAIKVDEGQKYYFGNITWKGNTKYSDSVLSLILGI
                     HKGDVYNVDILNKKLGKQMSQEGGDISGLYMDDGYLFFHVDPVETAVYNDTIDFEIRM
                     VEGPQATIKNVTISGNDKTNEYVIRRELFTIPGDKFSRTELIRSQRQIANLGFFNQEK
                     INPGVVPNQEDGTVDINWGVEEKSGNQLELSAGWGGGIGLTGTVGITFANFSIKNIFK
                     KDAWQPLPEGDGQKLSLRIQSNGKAYQSYNFSFT"
     gene            complement(1852..2625)
                     /locus_tag="VGQ51_18420"
     CDS             complement(1852..2625)
                     /locus_tag="VGQ51_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV9038621.1"
                     /translation="MDVLKENIDLKRLPRHIAIIMDGNGRWAQEKGQDRLFGHFHGVE
                     SVRDIVEGCAELGIGYLTLYAFSTENWDRPAYEVTGLMELLVETIRKEVGTLNKNNIR
                     LHVIGDMTMLPEYASVCLKEALELTSANTGLNLVMALSYSSRWELTEAVRNLAHEVQN
                     GNLSPEDITQDTLQHFLSTRDFPDPELMIRTSGEYRISNFLLYQLAYAELYFTSVRWP
                     DFRKNDLYEAILDFQGRERRFGKTSAQIQQDLMAPGSTF"
     gene            complement(2682..3533)
                     /locus_tag="VGQ51_18425"
     CDS             complement(2682..3533)
                     /locus_tag="VGQ51_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HEV9038622.1"
                     /translation="MKKLIFGILVVLSVGKRVTAQEQAITQEGEMGISVGAAQYFGDL
                     NPNPRFNTPNLAVSGFFRKNLNNYVALRVAASYAYLAYSDQHNAYNEFMYRRNLSFNT
                     NVWELAVQGDFNFFKFIPGSNFHRFTPYITLGAGIFNYNPYTYYQGQKIYLRQMGTEG
                     QGSAAYPDRKPYGSMAVCFPVGVGFKYSLNTHMNVGFEVLYRFTTSDYIDDVSKTYAP
                     NAQLQFLPNGQPTLWYALQDRSNETGEPIGIQGRQRGYSNQKDSYLTAQIFLSFNLTG
                     YKCPSAQ"
     gene            complement(3651..>4150)
                     /gene="ppnK"
                     /locus_tag="VGQ51_18430"
     CDS             complement(3651..>4150)
                     /gene="ppnK"
                     /locus_tag="VGQ51_18430"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788723.1"
                     /note="catalyzes the phosphorylation of NAD to NADP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEV9038623.1"
                     /translation="SPIHLDANRPLFGEVPYGLNEFAIHKTDTSPMIKIHTYLNGEFL
                     NTYWADGLIVATPTGSTGYSLSCGGPVVFPDSASFVITPVAPHNLNVRPIVVPDNNII
                     SFEVEGRAENFICVLDSRKEIVDKQVQLAVRNEAFTLSLIRLNENNFLQTLRSKLSWG
                     LDTRN"
BASE COUNT          938 a         1025 c         1118 g         1069 t
ORIGIN      
        1 cggtgaatga gaagttgtag gactggtagg ccttaccgtt ggactggata cgaaggctca
       61 acttctgacc gtcgccttcc ggcaggggct gccaggcgtc ctttttgaaa atgttcttga
      121 tcgaaaagtt ggcaaaggtg atccccacgg tacccgtcag accgatgcca ccgccccatc
      181 cggcggacag ttcgagctgg ttaccggatt tttcttccac gccccagttg atatccactg
      241 ttccgtcctc ctgattgggc accacgccgg gattgatctt ctcctggttg aagaagccga
      301 ggttcgcgat ctgccgctgc gaccggatga gctccgtgcg gctgaacttg tcgccgggga
      361 tggtgaacag ctcgcgccgg atcacatatt cattggtctt atcgttaccc gaaatggtca
      421 cgttcttgat ggttgcctgt gggccttcca ccatccggat ctcgaagtcg atcgtgtcgt
      481 tataaactgc cgtttccaca gggtcgacat ggaagaacag atagccgtca tccatataca
      541 gcccgctgat atcaccgcct tcctgcgaca tctgtttacc cagcttctta ttgaggatgt
      601 ccacattgta aacgtcccct ttatggatac cgaggatcag gctcagcacg gagtcggagt
      661 atttggtatt tcctttccag gtgatgttgc cgaaatagta tttctgtcct tcatcgacct
      721 tgatagcgat catcaggtgt ccttttccct gccggctggt atcgacgtaa ttggtgtcgg
      781 ccacgacctg cgcgtcgcga tagccctgcg agttataata ggtcaccacc ttctctttgt
      841 catcctcgaa tttggaggcg ttgaacttgg ccgagctgaa catcttgaac cggaagtatg
      901 gatcgatgaa ttcctttgtg ctggtgaagg acaggaaacc ccagtggttg aggtattgtt
      961 tcagcgtcac agagtcatgc gggccgtaag gtgtaatatt gctcggcggg tagaggctga
     1021 tacggctcaa ttccttggta tccttgagct gctttttcag cttggcctcg cggatcgcat
     1081 cgttgccgaa gaaattgatc tggtagatgc ggaccttggg acccctgtcg atcaggaagg
     1141 tgaggacctc ggaattctgc accgcagggt cacgggtttc ggtcgtgcgt atccgggcgt
     1201 cctggtagcc cttatcggca tagtattttt tgatggcctg gatagcggag cgtttcatgt
     1261 cctccgttat tacacgaccc ttgatcagac ccgtcttcgt tgccaggtcg tccgaagctc
     1321 ctttattcac gccggatccc ttgaaataga aattggaaag cctgggtctc tccgtcacat
     1381 tgatctcgac ataaaggctg gtaccggcca gtttggtgaa atagatagag atattcgcga
     1441 agaggtgttg cttccaaaga ttttgaattg ccttgctgaa attatcgccg ccggggatcg
     1501 tcaccttgtc gcctaccgtc aaacccgaaa tggaagtaag aagggattcg tccaaatatc
     1561 ttgttcctga gacctttatg ccggcgatcg tatattctct gggaggggtc ttgccgctgg
     1621 ccagcatgac cagctccgga tctatggaaa caggatgggt agtatcggtt tgtgctttta
     1681 aagaatccgt gatatgtgca tttttggtag tatccgtcac ctgggcttga gccgtgatcg
     1741 agttcagtaa gcagcagcaa atggtcaata aaaaaacgca gatcagcggt cttcgtagca
     1801 tccagtcggt ttttgagggg gcaaattaag gggatttatg cgaagtgttt gttaaaaagt
     1861 actgcccgga gccatcaaat cctgttggat ctgagcgctt gtcttaccaa atctgcgctc
     1921 tcttccctga aaatccaaaa tggcttcgta aaggtcgttc ttacggaaat cgggccaacg
     1981 aacactcgtg aaatataatt cggcatatgc gagttgataa agcaggaaat tgctgatacg
     2041 atattcgccg ctggtccgga tcatcagctc cggatcgggg aaatcccggg tcgaaaggaa
     2101 gtgttgcagc gtatcctggg taatgtcttc gggggaaagg ttaccattct gcacttcatg
     2161 cgccaggttc cggacggctt cggtcagttc ccaccggctg ctgtagctga gcgccatcac
     2221 gagattgagg cccgtattgg ccgacgtaag ctcgagcgct tctttgagac agacgctggc
     2281 atattccgga agcatggtca tgtccccgat gacatgcaga cggatattgt tcttattcag
     2341 cgtcccgacc tctttccgga tagtctccac caggagctcc atcaggcctg taacctcata
     2401 ggccggccgg tcccaatttt cggtagaaaa agcataaagg gtaagatagc cgatgccaag
     2461 ctcggcgcaa ccttcaacaa tgtcccgcac gctttcgacc ccgtgaaaat ggccaaagag
     2521 acgatcctga cctttttcct gggcccaccg tccatttccg tccataataa tggcgatatg
     2581 gcggggaagc cttttcagat cgatattttc cttcaacaca tccatattat ttgggcctta
     2641 tttattcggg ccttgttttc ggggcacaaa gatagggaat cctactgggc actgggacat
     2701 ttatacccgg tgaggttaaa ggaaaggaag atctgagcgg tcagatagct gtctttttga
     2761 ttggaataac cgcgctgacg gccctggata ccgatgggtt cgccggtctc attggaacgg
     2821 tcctgcagag cataccagag ggtgggctgt ccattgggga ggaattgcag ctgggcattg
     2881 ggcgcatagg tcttgctgac gtcatcgata tagtcgctgg tcgtgaaacg atagagtact
     2941 tcgaaaccta cattcatatg ggtattgagc gaatacttga atcctacccc caccggaaag
     3001 caaaccgcca tactcccata gggtttgcgg tcgggataag cagccgatcc ctggccttcc
     3061 gtacccatct gacggagata tatcttttgc ccctgatagt atgtgtaggg attataattg
     3121 aatatccccg cccccagggt aatatagggg gtgaaacggt ggaaattgct gccgggtatg
     3181 aatttgaaaa aattgaaatc gccctggacg gcaagttccc agacattggt attgaagctg
     3241 aggttccggc ggtacatgaa ctcgttgtag gcgttgtgct ggtccgaata agccaggtag
     3301 gcatagctgg cggctacccg cagcgccacg taattattta gattcttccg gaaaaagccg
     3361 ctgacggcga ggttgggggt attgaacctc gggttgggat taagatcgcc gaaatattgc
     3421 gctgccccaa cggaaatacc catctctcct tcctgggtga tcgcctgttc ctgcgccgta
     3481 acgcgctttc ccaccgaaag aaccaccagg atgccgaata ttagcttttt catgttgata
     3541 tgcgaaaata actccatacg aaacgaaacg aaacgccttt tcttttgcgc aattcctaat
     3601 tgttatgtta atgttgcgtg tgccttcggc gcaaactgca aaaggtgggc ctaattacgt
     3661 gtatccagtc cccaggaaag tttactgcgc agggtttgta ggaaattgtt ctcgttgagc
     3721 cggataaggc tcagcgtaaa ggcctcattc cggacggcaa gctggacctg tttgtccacg
     3781 atctccttcc ggctatccag cacacagata aaattttccg cccttccctc tacttcgaac
     3841 gatatgatat tgttatcggg cacgacgatc ggccggacat tcagattatg cggggcaacg
     3901 ggggtgatca cgaagctggc cgaatccggg aagaccacag gtcccccaca gctgagcgaa
     3961 tatcccgtcg aaccggtagg cgtcgcgacg atgagcccgt cggcccaata ggtattgagg
     4021 aattctccat tgagataggt gtggatcttg atcatcggag aggtatcggt cttgtggatc
     4081 gcgaattcat tcagaccata agggacctcg ccgaagaggg gtcggttggc gtcaagatgg
     4141 atcggcgaac
//
LOCUS       DASYSV010000592         5587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_119201, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000592 DASYSV010000000
VERSION     DASYSV010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119201"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1741)
                     /locus_tag="VGQ51_18435"
     CDS             complement(83..1741)
                     /locus_tag="VGQ51_18435"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEV9038624.1"
                     /translation="MRNLLFVALIVTLAYAPRDPFATVHTQAGLISGTTSKDGSVHIF
                     RGIPFAAPPIGARRWRAPQPLTPWSGIRKCDAFGPSPMQAKPVPFGVYTSEFLIPAQP
                     ISEDCLYLNVWTAATNPADRRAVLVYIYGGGFVSGGSACPIYDGEAMAKKGVVFVSIN
                     YRVGIFGFFSHPGLTAESGHNASGNYGLLDQIAALRWVKENIAAFGGDPSNVTIAGQS
                     AGSMSVSCLVASPLAKGLFKKAIGESVASILNVPGFIQTPTLRQAEQQGAKVATQLNA
                     SDLNALRAIPADRLLTINIPRGPVIDGYVLPALPGEILREHHNNPVVLLTGWNDDDYF
                     LGAPKNAQQFREQLKQQFGPSADSALLFYPATDDATALISQEHLNRDMLVGVQNYSWA
                     NLQSQNGRVFVYRFRRRPPATADFIKYGAFHTAEVPYAYGNLAFTNRPWEPIDHQLSA
                     IMTSYWANFASTGDPNTSGDRNTSATPNTSRTPNTSRTPGTSSTPNTAAPSTSGTPST
                     LPHWPSYTLPTATTMILDESPHAASVPDKAALDWLIRWIAHSEK"
     gene            complement(1815..4592)
                     /locus_tag="VGQ51_18440"
     CDS             complement(1815..4592)
                     /locus_tag="VGQ51_18440"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEV9038625.1"
                     /translation="MPIDPAHYATILRFWRSVETFTLPDIPAKKKTEPLLLPDMPLPW
                     DAGELPAPKEGRKWRHTLYFHIVPKTTVINHLARLTGSNEFLDGVGGETCLSALTLDS
                     HGQPFGRGYSAAAFIYGIKAIREKRNAEVLTDLLITAQRDYCIRFGLVVGPPPDFEPT
                     APPTVTWTHLNKELEYLKRLARDDLPSVTPIRCISEQVAATTAPEAPFLNSYYLQDLD
                     CLIRHPQDIGRPLRIFLSPDPHATGRIHLLEPELLRKYVQTAPPPGRWPSNPCHGLYA
                     AQYAALNIALPHTPVSSAGPQPSAPAPALLGINGPPGTGKTTLLREIIAAIITDRARR
                     LLATDPANLFQQRRIQISERAGYYRPEPGIIGSDGIVVSGNNNAAIENISHELPLCVS
                     IDKESFPGAEYFSAFATNVREKPCWGMLSADLGRSQKRSDFIDKFWFNGDKGFGKWLR
                     RQYEDHDEASANAENLRSTATRLRSLLSEYDIAVAKVPAPDFGGLCREDIHRATPYAS
                     EHLNILRSHIFLESLRLHEFTISCNARCVYQNLASFVDMLKGKWKENIDGHLAEHLWN
                     TFFFCVPVVSVTLASFQRQFQKLGQGSIGWLLLDEAGQASPPSVCGALWRCRQCILIG
                     DTRQIPPVVTIPATLSRFLQNQFGIAHDDWSPSKHSAQYLADRVTTTGAYIQQNNTDP
                     IWTGIPLRAHRRCHEPMFSIANTIAYNGQMVQATPIAPALTAASAIPTAASAIPLIPA
                     VPSAIPSAAPSIPHIPSGPSAAPPISFILSAAPPIPLIPSGPSGWFDIRDDEALDGHA
                     IARELEFIDLFLQPLSRYPHPIFLISPFRSIADLCSTRYEKIPMLKCGTIHTFQGKEA
                     DIVILVLGTHPDSIAARKWAASGPNILNVAVTRARHRLYVVGNRATWSRHNYYNILAA
                     TLPVRPHGLLL"
     gene            complement(4815..4964)
                     /locus_tag="VGQ51_18445"
     CDS             complement(4815..4964)
                     /locus_tag="VGQ51_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEV9038626.1"
                     /translation="MGGLLYIIAVILIICWAVGYFGYHAGGIIHVLLVIAVIAILLRV
                     IRSAD"
     gene            5095..>5587
                     /locus_tag="VGQ51_18450"
     CDS             5095..>5587
                     /locus_tag="VGQ51_18450"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEV9038627.1"
                     /translation="MVYKVIGLMSGSSLDGLDIAYVHLQEQVGSSQQGPRTWGFQLLH
                     TVTYPYNEEWRQRLAGATGLSAMEYQLLHVEYGHYLGEQVLRFVEAFGLDYQVQLIVS
                     HGHTTFHWPARKLTAQLGDGAAIAATTRINVVSDLRAMDIALGGQGAPIVPIGEMLLL
                     PGYE"
BASE COUNT         1129 a         1197 c         2073 g         1188 t
ORIGIN      
        1 tggagggtac ggatttgtgc tttttggtgt acgccgggtg gaaggtacgg agagggatgg
       61 gtgcggtaaa gagttaaaac acctattttt cgctatgggc tatccatcgg ataagccagt
      121 ccagcgcggc cttgtcgggg acggatgcgg cgtgcggcga ttcgtcgagg atcatcgtcg
      181 tcgcggtagg gagggtatag gatggccagt ggggaagggt ggagggggtg ccggaagtgg
      241 agggggcggc ggtgttgggg gtggaggaag tgccgggggt tcgcgaggtg ttgggggtgc
      301 gggaggtgtt gggggtggcg gatgtgttgc gatcgccgga tgtgttggga tcgccggtgc
      361 tggcgaagtt ggcccagtag gaagtcatga tcgcggagag ttggtgatcg atgggttccc
      421 agggacggtt ggtgaaggcg aggtttccat aggcataggg gacttcggcg gtgtggaagg
      481 cgccgtattt gatgaagtcg gcggtggcgg ggggacggcg gcggaagcga tagacgaaga
      541 cgcggccgtt ttgggattgg agattggccc agctatagtt ttggacgccc acgagcatgt
      601 cgcggttgag gtgttcctgg gatattagtg ccgtagcatc gtcggtggcg ggatagaaga
      661 gcagggcgct gtcggcgctg ggaccgaatt gttgctttag ttgttcgcga aactgttgag
      721 cgttctttgg cgcgccgagg aaatagtcgt catcgttcca ccccgtgagc agcacgacgg
      781 ggttgttatg atgttcccga aggatctcgc cgggcagtgc gggaaggaca tatccgtcga
      841 taaccggtcc gcgtggaatg ttgattgtta gtagccggtc ggcggggatg gcgcggaggg
      901 cattcagatc ggaggcattc aactgggtgg cgactttcgc gccttgttgt tcggcttgcc
      961 ggagggttgg cgtttgtata aagccgggta cattcaagat tgaggcgacg ctttctccga
     1021 tggccttttt gaagaggcct tttgcgagcg gcgatgcgac gagacagctg acgctcatgg
     1081 agcctgcgga ttggccggcg atggtcacat tggaggggtc gccgccgaag gcggcgatat
     1141 tttcttttac ccagcggagc gcggcgatct gatccagtaa tccataattg cctgaagcgt
     1201 tgtgcccgga ctcggccgtc aggccgggat ggctgaagaa accgaatata cctacccgat
     1261 agttgatgct gacgaagacg acgccttttt tcgccatggc ctctccgtca tagatgggac
     1321 aggcgctgcc gccgctgacg aaacctccgc catagatata gacgaggaca gcccggcggt
     1381 ccgcgggatt ggtagcggcc gtccaaacgt tcaaataaag acagtcttcg ctgatgggtt
     1441 gcgcggggat cagaaattcc gatgtataaa cgccgaatgg aacgggtttc gcctgcatgg
     1501 gactggggcc gaatgcatcg catttgcgga tgccggacca gggggtaagg ggctgcggcg
     1561 cccgccaacg gcgggcgccg atgggaggtg cggcgaaggg tatcccgcga aagatgtgga
     1621 cgctgccatc tttgctggta gtgccggaga taaggccggc ctgagtatgg acggtcgcga
     1681 agggatcgcg gggggcgtat gccagagtga cgataagggc gacgaaaagc agatttctca
     1741 taaggtaatt taccgaattt ttcggctttt gccagtgatc cacgaagatg gatagggttg
     1801 agcggagctg ctgatcatag cagcagaccg tgcggtctga cgggcagggt tgcggcgagg
     1861 atgttgtagt agttgtgtct tgaccaggtg gcacgattgc cgacgacata gagacggtga
     1921 cgggcgcggg tgacggcgac gttgaggatg ttgggaccgg aggccgccca cttgcgggcg
     1981 gcgatgctgt cggggtgggt gccgaggacg aggatgacga tgtcggcttc ttttccctgg
     2041 aaggtgtgga tcgtaccgca tttcaacatg gggatcttct catagcgcgt gctgcagagg
     2101 tcggcgatgg atctgaaggg ggaaatgagg aagatggggt gagggtagcg ggaaagaggt
     2161 tggaggaaga ggtcgatgaa ttcgagttcc ctggcgatgg catggccgtc aagggcttcg
     2221 tcgtcgcgga tgtcgaacca gccggaggga ccggagggga tgaggggaat ggggggcgcg
     2281 gcggagagga tgaaggaaat ggggggcgct gcggagggac cggaggggat gtggggaatg
     2341 gagggtgcgg cggaggggat ggcggagggg acggcgggga tgaggggaat ggcgctcgcg
     2401 gcggtgggga tggcgctcgc ggcggtgagg gcgggggcga tgggggtcgc ttgaaccatc
     2461 tgaccattgt aggcgatagt attggcgatg gaaaacattg gttcgtgaca gcgtcgatgg
     2521 gcgcggaggg gtatgccggt ccagatggga tcggtgttgt tttgttggat ataggcgcct
     2581 gtggtcgtca ccctgtcggc caggtattga gcggaatgtt ttgacggcga ccagtcgtca
     2641 tgtgcgatgc caaattggtt ctgcaggaat cggctgaggg tcgcagggat ggtgacgacg
     2701 ggagggatct gccgggtgtc tccgatgagg atacattgcc ggcaacgcca tagcgctccg
     2761 cagacggaag gaggagaggc ctggccggcc tcatccagca atagccagcc gatagagccc
     2821 tgacccagct tttggaattg tcgttgaaag gatgcgagcg tgacggagac gaccggaaca
     2881 caaaaaaaga aggtattcca taggtgttca gcgaggtggc catcgatatt ctccttccat
     2941 tttcctttca gcatatcgac aaaacttgcg agattttgat atacacaacg ggcgttgcag
     3001 ctgatggtga attcatgcag ccggagactt tcgagaaaga tatgagatct taagatgttg
     3061 aggtgttcgg atgcataggg agtggcgcga tgtatgtcct cgcggcagag accgccgaag
     3121 tcgggggcgg gaaccttcgc gacggcgatg tcgtattccg acagtaatga ccggagccgg
     3181 gtcgcagtgg atcggaggtt ttccgcgttg gcactggcct cgtcgtgatc ttcatactgc
     3241 cggcggagcc atttgccgaa gcctttgtcc ccattgaacc agaatttgtc gatgaaatcg
     3301 ctgcgttttt gtgaacggcc gaggtcggcg ctgagcattc cccagcaggg tttttcgcgc
     3361 acattcgtcg caaacgccga aaaatactca gcaccaggga aggactcctt gtcgatgctt
     3421 acgcagaggg gcagctcatg agagatgttc tcgatggctg cgttattgtt cccggaaacg
     3481 acgatgccgt cgctgccgat gatgccgggt tcgggacggt aatagccggc gcgttcggat
     3541 atctggatgc gccgctgttg aaagagattg gcagggtcgg tggccaggag gcggcgggcg
     3601 cggtccgtga tgatagctgc gatgatctcg cggagaaggg tggttttgcc ggtgccggga
     3661 ggaccgttga ttccgaggag ggctggggca ggcgccgacg gctgcggtcc tgcagaggac
     3721 acgggagtgt gcggcaacgc gatattcaag gcggcgtatt gagcggcgta aagaccatga
     3781 caggggttgg aaggccatct gccggggggc ggtgcggtct gtacatattt ccgcaatagc
     3841 tccggttcga ggaggtggat cctgccggtg gcgtgtgggt cgggagacag gaagatccgg
     3901 agcggccggc cgatgtcctg cggatggcgg ataagacaat cgagatcctg caggtaataa
     3961 ctgttgagga aaggggcctc cggcgcggtg gtggcagcga cctgttcgga gatgcaacgg
     4021 ataggagtga ccgatggaag atcgtcgcgg gcgagacgtt tgagatattc gagctctttg
     4081 ttgaggtgtg tccaggtgac ggtcggaggg gcggtgggtt cgaaatcggg cggcggaccg
     4141 acgacaaggc caaaacgtat acaatagtct ctttgcgccg tgatcaacag atcggtgaga
     4201 acttccgcgt tgcgtttttc ccggatggcc tttattccgt agatgaatgc ggcggctgaa
     4261 tagcctctgc caaagggctg gccgtggctg tcgagggtca atgcggaaag acaggtttcc
     4321 ccgccgaccc cgtccaggaa ttcgttggag ccggtgagtc gggccaggtg gttgatcacc
     4381 gttgttttgg ggacgatatg gaaatagagg gtatgccgcc attttctgcc ttcttttggc
     4441 gcgggtaatt cgccggcgtc ccaaggcagg ggcatgtcgg ggagcagaag aggttcggtc
     4501 tttttcttag cgggtatatc cggaagcgtg aatgtttcga cggatcgcca aaaccgtaag
     4561 atggttgcgt agtgtgcggg atcgataggc ataaggcaat ggttgcgcta taccaatgta
     4621 cagaaaagaa aggggggtgg aaaaaaaatc cgcgatcggc gtaccggtaa ggttacgggg
     4681 tacgatgggc tggggcacgg tgaagggtgg cgaggtaaga acgcggggta cggcggcacg
     4741 atggcggggg tacgtggaag ggtcagtgat cacagggcgg gcggggtacg gtggcgggta
     4801 gggtttgcgg gcggtcagtc tgcgctccgg atgactcgca gcaggatagc gatcaccgcg
     4861 atgaccagca gaacatggat gatgccgccg gcatgatatc cgaaatatcc gacggcccag
     4921 cagatgatga ggatcacggc aataatgtag agtaggcctc ccataaaaag tattttaagg
     4981 ttagaagatg aattatataa gacaaaattt ctgccagtac tgaacttgtg tgaacggacc
     5041 tgttttcgcc tattgatggg gtctgatttc cccaagtgcc gtaatttcgc cgctatggtg
     5101 tacaaagtca taggtctcat gagcggtagt tcgctcgacg gattggacat cgcgtatgtc
     5161 catttacagg aacaggtggg cagctcgcag caaggacccc gaacctgggg ttttcagctt
     5221 ttgcataccg ttacctatcc ctacaatgaa gaatggcggc agcggctggc tggggcgacg
     5281 gggctctccg ccatggagta ccagctgttg catgtggagt atgggcatta tctcggggag
     5341 caggtattgc ggtttgtcga agccttcggt ttggattacc aggttcagct gatagtgagt
     5401 catggacata cgacctttca ctggccggcg aggaagctga ccgctcagct gggagatggg
     5461 gcggccatcg cggcgaccac gcggatcaat gtggtgagcg atctgagagc gatggatatt
     5521 gcgctgggcg ggcaaggggc tccaatagtg ccgatcgggg agatgttgtt gttaccgggt
     5581 tatgaat
//
LOCUS       DASYSV010000593         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2042459, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000593 DASYSV010000000
VERSION     DASYSV010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042459"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGQ51_18455"
     CDS             <1..240
                     /locus_tag="VGQ51_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038628.1"
                     /translation="ALQQVEAAIRNTGGKPLFIFYFSAVLFALGRTREGLLQLETGMS
                     KAPKLLKKLLELNPVILQYQPVVDIIARYKKNKSI"
     gene            270..1043
                     /locus_tag="VGQ51_18460"
     CDS             270..1043
                     /locus_tag="VGQ51_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEV9038629.1"
                     /translation="MNFKLTQMPERLQKPRQHGITMVMDKGLSIDEAKNFLSVAHPHV
                     DIVKLGFGTSFVTPNLREKIEVYRQYGLPIYFGGTLFEAFLIRNQFEDYIAICKDYGI
                     THMEVSDGSITIPHAEKCGYIEKLTKYGTVLSEVGSKDAAHIIPPYKWIELMSAELGA
                     GASYVIAEAREAGNVGIYRGSGEVREGLVQEILTQIPAEKILWEAPQKAQQLYFIELV
                     GCNVNLGNIAPTEVIAMEAMRIGLRGDTFHLFLDKDTAR"
     gene            1040..1774
                     /locus_tag="VGQ51_18465"
     CDS             1040..1774
                     /locus_tag="VGQ51_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV9038630.1"
                     /translation="MRRYGLIGFPLSHSFSQRYFTEKFQREGIKDCVYENFEIPTIDA
                     LKAVLADHGLCGLNVTIPYKQEVMPYLDERSAVVESIGACNCIRIEAGRLTGYNTDVV
                     GFEQTLVRRLGSHHRRALVLGTGGASKAVEWVLGKLGIEYRMVSRRPRGGSDDLSYEQ
                     VDAALVGEYTLIVNTTPLGMYPRTEECPPLPYEALTPRHYLFDLIYNPERTLFLRKGE
                     TRGAVVENGQEMLVLQAEESWRIWNG"
     gene            complement(1779..2210)
                     /locus_tag="VGQ51_18470"
     CDS             complement(1779..2210)
                     /locus_tag="VGQ51_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV9038631.1"
                     /translation="MPRTKFDLPAHFSFTTDVPVRITDINYGGHVGNDSMLSLIHEAR
                     VRFLSHFGYEELNLAGVGLIMTDVTIEFRNELFYGDSLRASICASDFSRVTFDLHYKL
                     EKKSGEKWIPVAFARTGMACFDYAAKKLTAVPKEVCTRLLT"
     gene            complement(2217..2804)
                     /gene="rdgB"
                     /locus_tag="VGQ51_18475"
     CDS             complement(2217..2804)
                     /gene="rdgB"
                     /locus_tag="VGQ51_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEV9038632.1"
                     /translation="MSTRIIFATNNENKVKEIRTAVGDALNIVSLKEAGIDIDIPEPW
                     DTLEANATEKSTTIHRLTGDNCFSEDTGLEVYALNNEPGVLSARYAGEQKSSRDNIAK
                     LLDRLGRKPDRRARFRTVISLIFDGKEHLFEGICEGHILQTPEGESGFGYDPIFAPEG
                     ENVSFARMSPEEKNGHSHRRKAADQLVSFLRQISK"
     gene            complement(2801..>3306)
                     /locus_tag="VGQ51_18480"
     CDS             complement(2801..>3306)
                     /locus_tag="VGQ51_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038633.1"
                     /translation="LVMDINHVQLSDLAIGELYGDALLVSAPLSDAAPAALKFLGNHR
                     RHFTILVHSPGSAFLPDDQLSFLTKILEACRMNIGDVAIINTATAPVAITHLKQQLHP
                     ETILLFGVEPTAIRLPINFAAFRPQPYDNCTYLSAPALDLLVQPGEEGRLLKSKLWVC
                     LKSLFNV"
BASE COUNT          705 a          740 c         1077 g          784 t
ORIGIN      
        1 gctcttcagc aggtggaggc ggccattcgc aataccgggg gcaagccgct gttcatcttt
       61 tatttttccg ctgtcctgtt tgcgctgggc cgtacacgcg aggggctgct tcaactggag
      121 actgggatgt caaaggcgcc gaagctcctt aaaaagctgc tggagctgaa tcccgtcatc
      181 cttcagtatc agccggtggt ggatatcata gcgcgataca agaagaataa atccatctga
      241 tcccggcgat tccctatttt tgcggcacta tgaatttcaa actcacgcag atgccggaac
      301 ggctgcagaa gccgcgtcag catggtatta cgatggtaat ggacaagggg ttgagtatcg
      361 atgaggcaaa gaactttttg agcgtagcgc atccccatgt ggatatcgtc aaattgggtt
      421 tcggcacttc tttcgtcact cccaatctcc gggaaaagat cgaggtctat cgtcagtacg
      481 ggctccccat ttatttcggg gggacccttt tcgaggcttt tctgatccgg aatcagttcg
      541 aagattatat cgccatctgt aaggattacg gcataaccca tatggaggtc agcgacggga
      601 gcattactat cccgcacgcg gaaaaatgcg gttatatcga aaagctgacc aaatatggaa
      661 cggtgctgag cgaagtgggc agcaaggacg cagcgcatat cattccccct tacaaatgga
      721 tcgagctgat gtccgcagaa ttgggcgcgg gtgcttccta tgtcatcgct gaggcgcggg
      781 aagccgggaa tgtgggcatt taccgcggca gcggagaggt ccgtgagggc ctggtgcagg
      841 agatactgac gcagataccc gcagagaaga tcctgtggga ggctccgcag aaggcgcagc
      901 agctgtattt tatagagctg gtcggctgta acgtcaatct cgggaatatc gctccaaccg
      961 aagtcattgc catggaggcg atgcggatag gtcttcgcgg ggatacattt catcttttcc
     1021 tggataagga tactgcgcga tgagacggta cgggttgata ggattcccct tgtcgcattc
     1081 tttttctcag cgatatttta cggagaaatt tcagcgtgag gggataaagg attgcgttta
     1141 cgagaatttt gagatcccca cgatagacgc tttgaaggct gttttagcag accacgggct
     1201 gtgtggacta aacgttacga tcccttataa gcaggaggtg atgccgtacc tggacgaaag
     1261 gagtgcggtg gtggaatcga taggcgcttg taattgtatc cgtatcgagg cgggtcggtt
     1321 gaccgggtat aataccgacg tagtgggttt tgagcagacg ctggtcaggc gtcttgggtc
     1381 gcatcatcgc cgggcgctgg tccttggaac gggcggcgca tccaaagcgg tggaatgggt
     1441 gctggggaag ctgggtatcg aataccggat ggtgagccgg cggccgcggg gagggagtga
     1501 cgatcttagc tatgagcagg tggatgctgc gttggtgggt gagtatacgc tcattgtgaa
     1561 tacgacgccg ctgggtatgt acccaaggac ggaggagtgt cctccgttac cctacgaggc
     1621 gttgacgccg cggcattatc ttttcgacct gatctacaat ccggagagga ctctgttcct
     1681 gcggaagggg gaaacccggg gcgcggtcgt ggagaacggg caagagatgc tggtcctgca
     1741 ggcggaggag agctggagga tatggaacgg gtgagttttc aggttagaag gcgggtacag
     1801 acttctttcg ggacggcggt gagctttttc gccgcgtaat caaaacaggc catgccggtg
     1861 cgggcgaagg cgacggggat ccatttttct ccggattttt tttccagttt ataatgcaga
     1921 tcgaaggtga cgcgcgaaaa gtcggaggcg cagatcgagg ccctaaggct atcgccgtag
     1981 aagagctcgt tcctgaactc gatagttacg tcggtcatga tgaggcctac gccggcaagg
     2041 ttcaattctt catatccgaa atggctgaga aagcgaacgc gagcttcatg gataagcgag
     2101 agcatcgaat cgttgccgac atgtccccca taattgatat cggtgatgcg aacggggaca
     2161 tcggtggtga acgagaaatg tgcgggtaag tcgaattttg ttcttggcat atgcaatcat
     2221 ttagagattt ggcgcaggaa gctcaccagt tgatcggcgg ctttgcgccg gtggctatgg
     2281 ccgttctttt cctccggcga catacgggcg aatgaaacgt tttctccttc cggcgcgaat
     2341 atcggatcat aaccgaatcc gctttcacct tcgggggttt ggaggatgtg gccttcgcag
     2401 atcccttcga acaggtgttc ctttccatcg aagataagcg atataactgt gcggaagcgc
     2461 gcgcggcggt cgggtttccg gccgagccgg tcgagcaatt tggcgatgtt gtcgcgggag
     2521 gatttttgtt cgccggcata acgtgcgctg agcacgccgg gttcgttgtt gagcgcgtag
     2581 acttccagac cggtgtcttc gctgaaacag ttgtcgcccg tgagacggtg gatggtggtg
     2641 gatttttcgg tggcgttggc ttcgagggta tcccatggct cggggatatc gatatcgata
     2701 cctgcttctt tcaggctgac gatgttcagc gcgtctccga ccgcggtgcg gatctctttt
     2761 actttgtttt cgttgttggt agcgaagatg atgcgggtac tcatacgttg aacagtgatt
     2821 ttaaacagac ccagagcttg ctcttcagca gcctgccctc ttcgcctggc tgcacgagga
     2881 ggtccagtgc gggtgcggaa agataggtgc aattgtcgta gggttgcggc cggaaggccg
     2941 cgaaattgat cggcagacgg atggcggtgg gttccacgcc gaagagcagg atcgtttcgg
     3001 ggtggagctg ctgtttcaga tgggtgatgg cgacgggtgc ggtggcggta tttatgatgg
     3061 ccacgtcgcc gatgttcatg cggcaggctt cgaggatctt ggtgaggaag gatagctggt
     3121 cgtcgggaag gaaggcggag ccgggcgaat ggacgaggat ggtgaaatgg cggcggtggt
     3181 tgccgaggaa tttgagagcg gcgggtgcgg catcggatag gggtgcggag accagcagcg
     3241 catctccata gagttcgccg atggcgagat cagacagctg aacgtgattg atatccatga
     3301 ctaacg
//
LOCUS       DASYSV010000594         5984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1146538, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000594 DASYSV010000000
VERSION     DASYSV010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146538"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VGQ51_18485"
     CDS             <1..522
                     /locus_tag="VGQ51_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEV9038634.1"
                     /translation="PNFMEVQYSPSESQWAELREGQLLALSVPNTAMAVAIDAGEWND
                     IHPLDKKDVGDRLALAAEHLAYGDKDVVYSGPIYRSGSIAGDSIVLNFDHTGSGLMAK
                     GGGPLHGFAIAGADKKFVWADARIEGDHVVVSSDEIREPKYVRYAWADNPVFANLYNK
                     EGLPASPFGTDQE"
     gene            531..2063
                     /locus_tag="VGQ51_18490"
     CDS             531..2063
                     /locus_tag="VGQ51_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038635.1"
                     /translation="MKKNNLFIVLLTIAVPAVAQQASTGQYSAPVNFTAQQDHDNMMK
                     QLGIKTLRPGPSGDEKAPNHANYDPALANPYPNLPDILTLNNGKKVTTPDEWWKQRRP
                     EIVEDFEREVYGRVPAHVPKVTWTVRVTDHEFVGFFMPVIAKQLIGHVDNSEYPLIDV
                     NIKATLVVPANAKGPVPLLMMFGQSVLPAPAQPPADDVEKINEAIKELLVKEHPEFKE
                     IFEKYPAYNPIATQGPPGFGGPRPAGDPPTTQQLIANGWGYILIDPNSIQADNGAGLT
                     RGIIGLCNHGQPRKPEDWGALRAWAWGAARALDYLEANEPEVDSKHVGIEGVSRYGKA
                     ALVTLAFEPRFYMGLIGSSGEGGAKLNRRNFGEAVESLTGGEYYWMAGNFMKYGASDA
                     VFGAKTANDLPVDSHELIALCAPRLCFVSYGLPSQGDANWLDHQGSYMATVAAGVVYK
                     LLGAKDLGVSNDYHTEKMPPVNTGLLDGELAWRQHDGGHTDAPNIKYFIQWADKFMGW
                     KK"
     gene            complement(2044..3177)
                     /locus_tag="VGQ51_18495"
     CDS             complement(2044..3177)
                     /locus_tag="VGQ51_18495"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV9038636.1"
                     /translation="MSTSTHTLIIGASVAGLATAAALGRRQIPYCILERHGDIAAPWR
                     SHYHRLHLHTPKSISHLPFKEFGKNIPRYPSRKQVVDYLDEYRGAFGIEPLFDTEARK
                     IRWEGGQWITETDKGIFRSVNLVIATGVYDKPRSIDCTGIDTFPGPVLHSGDYVTGKT
                     FSGRKVLVIGFGNSACEIAIDLYEQGAIPSLSVRSPVNVVPRDVFGIPILRLSYFLDA
                     LPPRIADRLSGPLMRWLIGDITRLGLQRKSYGPLEEIRRDGHPPVLDIGTVRHIREGH
                     IKVYGGIDHIDGPTVYFSDWKQGAFDAIVGAIGYDSGYARLLDVDETRLVDARLPIPR
                     QKYFGVDGLYFCGTRVSPTGQIRSICSDAQKIAKHISLTSSIP"
     gene            complement(3174..3839)
                     /locus_tag="VGQ51_18500"
     CDS             complement(3174..3839)
                     /locus_tag="VGQ51_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV9038637.1"
                     /translation="MEHIKKILSITPVSHDVRCIRIEKPDQYSFMPGQATDVALDKDG
                     WREEKRPFTFTSLNSDPYLEFTIKSYTDHDGVTKQIGMLEPGDHLIIDEPWGAIHYKG
                     EGYFIAGGAGITPFMAIFRQLHQVGLIGKSKLFFSNKTAADIIYRTELLKIFGGNAFF
                     VLTQKGEEAYARDFIDRAFLQSHVNQMNKYFYVCGPDAMVKGLNKILEGLGANPQSVV
                     FEK"
     gene            3896..4336
                     /locus_tag="VGQ51_18505"
     CDS             3896..4336
                     /locus_tag="VGQ51_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV9038638.1"
                     /translation="MKRKLYLTGCAVFILLGTLIVRGRQTDPWSADQLMPPSELAAKI
                     NNNSDPLVICVGPAGLIKGSVETGAAHETAGLEKLKILLSGVDRNKEVIIYCGCCPFK
                     NCPNVRPAFNLLLSMHFTHPRLLDIPHNIRVDWIEHGYPLKDAK"
     gene            complement(4343..4987)
                     /locus_tag="VGQ51_18510"
     CDS             complement(4343..4987)
                     /locus_tag="VGQ51_18510"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV9038639.1"
                     /translation="MIYQEPVQMRQIPDSALIDNVLSGDKPAFEHLIRRYNQRLFRIG
                     MSMLGNESDSEDAMQSSYINAWLHLAQFRRDASFGTWLTRIMLNQCLEQQRKNRLIKT
                     TIVQSDNVMNTRTPGSELANKELSSLLENAMSRLPEKYRLVFVMREVEDLSVKETSEV
                     LDIGVSNVKTRLNRAKAMLREDLKGYMRENVYAFDLKRCDRIVAGVFAAIAGLP"
     gene            5107..5481
                     /locus_tag="VGQ51_18515"
     CDS             5107..5481
                     /locus_tag="VGQ51_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038640.1"
                     /translation="MATVKQLQGVLRFTYGIVPIVAGLDKFTNLLCNWSDYLGTNKSM
                     VPMEAMTFMKIVGVIEIIAGIIVFARTLLGAYIVMIWLILIALSLLIGTHYFDVAVRD
                     LVMAVGAFTLAQLTRITSSGKV"
     gene            5608..>5984
                     /locus_tag="VGQ51_18520"
     CDS             5608..>5984
                     /locus_tag="VGQ51_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEV9038641.1"
                     /translation="MDTNLKRASAAGVIVALGIIYGDIGTSPLYTLQTILNEGGPIDK
                     SLVFGSISCIFWTLTLQTTFKYVVITLQADNHGEGGVFSLYSLIRRYGRRLVYPAIIG
                     AGTLLADGIITPPITVTSAIEGLG"
BASE COUNT         1377 a         1600 c         1647 g         1360 t
ORIGIN      
        1 ccgaatttca tggaagtgca atactcgcca tcggagagtc agtgggcgga attgcgcgag
       61 ggacagctgc tggcgctgtc ggtgccgaat acggcgatgg ccgtcgcgat cgatgccggg
      121 gagtggaacg acattcatcc actcgacaaa aaggacgtgg gcgacaggct ggcgcttgcg
      181 gcggagcacc tggcttatgg agacaaggat gtggtctatt caggtcctat ttaccggagc
      241 ggatcgatcg cgggcgatag tatcgtgctg aacttcgatc ataccggcag cggtctgatg
      301 gccaaaggcg gaggcccgtt gcatgggttc gccatcgcgg gtgcggataa gaaattcgta
      361 tgggcggacg cgcggatcga aggcgaccac gtcgtcgtct ccagcgatga gatacgggag
      421 ccgaagtatg tccggtatgc ctgggccgac aatcctgtgt tcgccaattt atacaacaaa
      481 gaaggattac ccgcatcgcc gttcggaacg gatcaagaat aatttcgcgt atgaaaaaga
      541 ataatttatt cattgtcttg cttactatcg ctgtacctgc cgtggcgcag caggcgtcca
      601 cgggccaata tagcgcgccc gtcaacttca ctgcgcagca ggatcatgac aacatgatga
      661 aacagctggg aataaaaacg ctacgtcccg gccccagcgg agacgaaaaa gcgccgaatc
      721 atgccaatta cgacccggca ctcgccaatc catatcccaa tctccccgat atcctgaccc
      781 tcaacaacgg caaaaaagtc accacgcccg atgagtggtg gaaacaacgc cggcccgaga
      841 tcgtggagga cttcgagcgg gaggtatatg gcagggtacc ggcgcatgta ccgaaggtta
      901 cctggaccgt cagggtgacg gatcatgagt tcgtcggatt tttcatgccg gtgatcgcca
      961 aacagctgat cggacatgtc gacaattccg aatatccgct catcgacgtc aatatcaaag
     1021 cgaccctcgt ggtgccggca aacgcgaagg gtcccgttcc attgctgatg atgttcgggc
     1081 agagcgttct tcccgcaccc gcgcaacctc cggcggatga cgtggaaaag atcaacgaag
     1141 ctatcaagga attactcgta aaggaacatc ccgagttcaa ggaaatattt gaaaaatacc
     1201 ccgcttataa cccgatagcc acgcagggac cccccggttt cggaggaccc cggcctgcag
     1261 gtgaccctcc taccacccaa cagctgatcg ccaacggatg gggatatatc ctgatcgacc
     1321 cgaacagcat acaagctgac aacggcgccg gtttgaccag gggcatcatc gggctctgca
     1381 atcatgggca accacggaaa cccgaagact ggggcgcgtt gcgtgcgtgg gcgtggggag
     1441 cagccagagc gctggattac cttgaagcca acgagccgga ggtcgacagc aagcatgtag
     1501 gcatcgaggg cgtatcgcgt tatgggaaag cggccctcgt gacgctggca ttcgaacccc
     1561 gattttatat gggtctcatc ggatcgtcgg gggaaggcgg ggcgaagctc aaccggcgca
     1621 atttcggaga ggctgtagaa agtctcaccg gcggcgaata ctattggatg gccggtaatt
     1681 tcatgaaata cggggcttcc gatgccgtct ttggggcgaa gaccgctaac gatcttcccg
     1741 tcgactcgca tgagctgatc gcgctgtgcg ctccgcgact ttgtttcgtc agctatggcc
     1801 ttccgtcaca gggcgacgcc aactggctgg atcaccaggg cagctatatg gccaccgtcg
     1861 ccgcgggagt ggtctataag ctgctaggcg caaaggatct gggcgtctcc aatgactatc
     1921 acaccgaaaa aatgccgccc gtcaataccg gtctgctcga tggggagctt gcctggcggc
     1981 agcatgacgg tggtcatacg gatgcgccta atatcaaata ttttatccaa tgggcggata
     2041 agttcatggg atggaagaag tgaggctaat gtgcttcgcg atcttttgcg catccgagca
     2101 tatcgagcgt atctgacccg tagggctgac ccgggtaccg caaaaataca atccatccac
     2161 gccaaaatat ttctgccggg ggatcggcag tcttgcgtcg acgagacggg tctcgtcaac
     2221 gtctaacagt cgcgcatagc cggaatcgta gccgatcgcg cccacgatag cgtcgaaggc
     2281 cccctgtttc caatcgctga aatacactgt cggcccatcg atatgatcga tcccgccata
     2341 gactttgata tggccctcgc ggatatgccg gaccgttccg atatccaaca ccgggggatg
     2401 gccgtcgcgc cggatttctt ccagcggtcc atacgatttc cgctgaagac ccagacgggt
     2461 tatatcaccg atgagccatc gcatcaatgg cccgctgagg cggtcggcta ttcgcggtgg
     2521 caacgcgtcc aggaaatagc tcagacgcag tatcggaata ccgaacacgt ctcttggcac
     2581 gacgttgacc ggtgagcgta ccgacaatga aggtattgcg ccctgttcat acagatcgat
     2641 cgcgatctcg caggccgaat tgccgaagcc gatgaccagc accttgcggc cgctgaaggt
     2701 tttacctgtc acataatcgc cgctatggag taccggcccg gggaaggtgt cgatgcccgt
     2761 gcaatcgatg gaccggggtt tgtcatacac gccggtggca atgaccaggt ttacgcttcg
     2821 aaaaatgccc ttatccgtct ccgtgatcca ctggccgccc tcccatctaa tctttctagc
     2881 ctccgtatcg aacagcggtt cgatcccgaa ggcgccccgg tactcgtcga gataatcgac
     2941 cacctgcttt cgcgagggat aacggggtat attcttaccg aactctttaa agggtaaatg
     3001 ggagatggat ttaggagtat gtaaatgcag ccggtgataa tggctccgcc agggtgcggc
     3061 tatatcgcca tgtctttcga gaatgcaata aggtatttgc cgccgcccca gggcggcggc
     3121 agtagcgaga cccgccacgc tggcaccgat gatcagcgta tgggtggaag tgctcatttt
     3181 tcaaaaacga cagattgcgg attggcaccc agtccttcca gtatcttgtt gagtcccttt
     3241 accatggcat ccggtccgca aacgtaaaag tatttgttca tctggttaac atgcgattgc
     3301 aaaaaggcgc gatcgataaa atcccgggcg taggcctcct cccctttttg ggtcagcaca
     3361 aaaaaggcgt tgccgccgaa tatcttcagc aattccgttc tgtaaatgat gtcggcggcc
     3421 gtcttattgg aaaaaaagag tttgctcttg ccgatgagtc ccacctgatg cagctggcgg
     3481 aagatggcca tgaagggggt aatgcccgct ccgcccgcta tgaaataccc ttctccttta
     3541 tagtgaatgg cgccccaagg ctcatcgatg atcagatggt cccccggctc cagcatacct
     3601 atctgctttg tcaccccgtc atggtcggtg taggatttga tggtgaattc caggtaaggg
     3661 tcgctgttga ggctggtgaa tgtaaagggt cgtttctcct cgcgccagcc atctttgtca
     3721 agggccacat ccgtagcctg accgggcatg aatgaatatt ggtctggctt ttcgatccgg
     3781 atgcaccgga cgtcatggga aaccggggta atagataaaa tcttctttat atgctccatt
     3841 gttgcgctat ttcgtccgat aatcttaatt tagcaaggac caatagaact acaaaatgaa
     3901 acgcaaactt tatcttaccg gttgcgccgt cttcatcctt ttgggtacct tgatcgtcag
     3961 gggtcgacag accgatccct ggtcggcgga tcaactcatg cctccttccg agcttgccgc
     4021 caaaatcaat aacaattcgg atccgctggt catctgcgtg ggtcccgcag ggctcatcaa
     4081 aggctccgtc gaaacaggcg cggcgcatga gaccgcgggt ctcgaaaaat tgaagatatt
     4141 gctttccggg gtcgaccgca acaaggaggt gatcatttac tgtggttgct gtccctttaa
     4201 gaactgtccg aacgttcggc ctgccttcaa cctgctgctc tcgatgcatt ttacgcatcc
     4261 gcggctgctg gatatcccac ataatatccg cgtcgactgg atcgagcatg gatatccgct
     4321 caaagatgcg aaatagggcg agctacggca gaccggcgat ggccgcgaag actccggcca
     4381 cgatacgatc gcatcgtttc aaatcgaagg cataaacatt ctccctcata tatcctttca
     4441 gatcttcgcg cagcatcgcc ttggcgcggt tgagacgcgt cttgacattg gagacgccga
     4501 tatcgaggac ctccgaagtt tccttcacgg acagatcctc tacttctctc atcacaaaca
     4561 cgagccggta tttttccggc aggcggctca tggcgttttc caatagggaa gaaagctctt
     4621 tgtttgccag ctcgctgccc ggtgttctcg tattcatgac attatccgat tgtacgatcg
     4681 tggttttgat cagcctgttc tttcgctgtt gctcaagaca ttggttcagc atgatcctcg
     4741 tcagccaggt accgaaagaa gcgtcgcgtc tgaactgagc gagatgcagc caggcattga
     4801 tataggacga ctgcatggcg tcctccgaat cggactcgtt gccaagcatc gacatcccga
     4861 tccggaacaa acgctgatta tatcggcgga tcaggtgctc gaacgccggt ttatctccgg
     4921 ataaaacatt gtctattaat gcgctatccg gtatctgtcg catttgaacg ggttcctggt
     4981 agatcataag ctaagattgg atgaacgaag gccgtaaagg tcacaaatct cgcaagaaaa
     5041 ttgcatagta ttttttgtaa cgcaatcagg actcattgat ctaatggtaa acaaattact
     5101 cattttatgg ctaccgtaaa acaactacaa ggcgtattaa gattcactta tggtatcgtg
     5161 cctatcgtgg cagggctcga caaattcacc aatctgctgt gcaattggtc cgactacctt
     5221 ggcactaata aatcgatggt tccgatggag gcgatgacct tcatgaagat agtcggtgtg
     5281 atcgagatca ttgccgggat catcgttttt gcgaggaccc tcctcggggc ctatatcgtt
     5341 atgatatggc tgatactgat cgccctgtca ttgctgatcg ggacacatta tttcgacgtg
     5401 gccgtgcgcg acctggtgat ggctgtaggc gcctttacgc tcgctcagct gacccggata
     5461 acatcctccg gcaaggtcta aacgtgtgcc cggcgccgcg ggatggacgt gcattgggca
     5521 actccgatgc cgaagcgatt gccgtgcgtt tgacgcctgc ggatgacgca gggtggcggt
     5581 ggcccgattt tgggtgagtc taccggtatg gataccaatc tcaaacgggc gtctgccgcg
     5641 ggtgtcatcg tggcgctggg catcatttat ggagatatcg gcacttctcc gctgtatacg
     5701 ctgcagacga tactcaacga gggaggaccg atcgacaaat ccctggtatt cggctctatc
     5761 tcctgcatct tctggacgct gacgctgcaa acgactttca aatatgtggt aatcacgcta
     5821 caggcggata accacggaga gggaggtgtc ttttcattgt attcgctgat ccgccggtat
     5881 ggaagacgcc tggtataccc tgccatcatc ggcgccggca cgttactggc ggatggcatc
     5941 atcacccctc ccatcaccgt tacatcggcg atcgaaggat tggg
//
LOCUS       DASYSV010000595         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1918782, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000595 DASYSV010000000
VERSION     DASYSV010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918782"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..821
                     /locus_tag="VGQ51_18525"
     CDS             81..821
                     /locus_tag="VGQ51_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9038642.1"
                     /translation="MPTVITTDKLTKRFGSFVAASELTFTVEKGEIFGFLGANGAGKT
                     TAMRMLCGLLKPSSGNATIAGFDVYSETEKIKRNIGYMSQKFSLYDDLTVSENIRFYA
                     GIYGMSLPAIREKTRVLLDELQLRNEAKTLVRELPLGWKQKLAFSVSIVHDPEIVFLD
                     EPTGGVDPVTRRQFWDLIYSAADKGVTVFVTTHYMDEAEYCNRVSIMVDGVIAALDSP
                     ATLKKQFGGASMEDVFYELARNAKRKGD"
     gene            823..1929
                     /locus_tag="VGQ51_18530"
     CDS             823..1929
                     /locus_tag="VGQ51_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038643.1"
                     /translation="MRQLIIFIRKEFLHVFRDRKTLLMLFGLPIAQIVLFGFALSNEI
                     KDSNIALVDYSKDAVTTRLIGKIEASHYFDIRRTLADATQLQAAFKKGDIKMAVIFPA
                     NFGNDLMHTGKATLQVIADASDPNQATTITNYLQQVVADYNNELAAGAQQPLRIVPQM
                     QMLYNPELRGAPNFVPGVMALVLMLVCTMMTAVAIVKEKELGTMEVLLVSPFKPVYII
                     ISKLMPNLIISVVNLVIILVLSNVFLDLPIRGSTLLLIFCSILFILTCLSIGLLVSIG
                     AKTQQTAMMGSMMGMMLPTMLLTGFLFPIENMPVVLQVLSNLVPSRWYFIIIKRVMLK
                     GLGFGAIWKETLILAGMCVFLFTISLRNFKIRLQ"
     gene            1926..3047
                     /locus_tag="VGQ51_18535"
     CDS             1926..3047
                     /locus_tag="VGQ51_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038644.1"
                     /translation="MRTLRFLLEKEFRQLLRNRQLVRTLLLAPLIQLILLPMAADYTV
                     KNIHIVVADNDHSPSSRRLIAKITSSGYFHLSGYTASYPEALRMVEQDKADLALQIPA
                     HFERDLVRENANQLYIAINSIDGTKGNLGGAYLRSIITDFNDDLRVDMKTVEPVQEDP
                     PQVSVASSNWYNPFLKFPLYMVPAILVTLVTAMVAMQTAFNIVQEKESGTIEQINVTP
                     IKKYIFITGKMIPFLVLGIILFTMGLLVGWALYGVVPLGNLWVLYGSLIVYLLSMLGL
                     GLLIATYSGTQMQAMSLAFFFINIFNMMSGVFTAVDSMPAWAQAIVATFPPSHFIRIM
                     RMVVLKGSGFGDIRYELVWMLVIGLFLNAWAIKNYRKIA"
     gene            complement(3079..>4243)
                     /locus_tag="VGQ51_18540"
     CDS             complement(3079..>4243)
                     /locus_tag="VGQ51_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEV9038645.1"
                     /translation="QQLVELYRAGKTVEDILRTDLQADYEQNASERLFPNVDGIKFLN
                     HDGGCGGTRADAQALCGLLAGYITHPNVAGATVLSLGCQNAQVSILEEEIRKRSPEFS
                     KPMYILEQQKIGTESAFLSEAIKQTFAGLILANQQERRPAPLSKLCIGLECGGSDGFS
                     GISANPAIGYTSDLLVAMGGSVILSEFPELCGVEQNLSDRCMDVPTAERFSHLMRTYN
                     DRAKAVGSGFDMNPSPGNIRDGLITDAIKSAGAAKKGGTSPVSDVLDYPEKVSKPGLN
                     LLCTPGNDVESTTAEVGSGANIVLFTTGLGTPTGNPIAPVVKIASNTALFDRMRDIID
                     INTGTIIEGNETIKEAGERILEYVIGVASGETQVAAVRHVQDDFIPWKRGVSL"
BASE COUNT          941 a         1279 c         1059 g          964 t
ORIGIN      
        1 aaaggacttc ccgacgcacg cctcaaaaag atcacacccg gtatcgaaga ttgttttatc
       61 catcttttaa aatcctgacc atgccaacgg tgatcaccac ggataaatta acgaaacgtt
      121 tcggctcttt cgtcgccgcc agcgagctga ccttcacggt agaaaagggc gagatcttcg
      181 gattcctggg ggcgaacggg gccggcaaaa cgacggcgat gcggatgctc tgcggattac
      241 tcaaaccttc ctccggaaac gccaccatcg ccgggttcga cgtctattcc gagaccgaaa
      301 agatcaaacg caatatcggg tatatgagtc agaaattctc gctctatgac gacctcacgg
      361 tcagcgagaa catccgtttt tatgccggca tttacgggat gagccttccg gccatccggg
      421 aaaaaacccg cgtccttctg gacgagctcc agctccggaa cgaagcgaag acactcgtcc
      481 gcgagctgcc attgggctgg aaacaaaagt tggctttttc ggtatcgatc gtccacgatc
      541 ccgagatcgt gtttctcgat gaacccaccg gcggcgtcga ccccgtgacc aggcgccagt
      601 tctgggacct gatctattcg gcggccgaca aaggcgtgac ggttttcgtg acgacgcact
      661 atatggacga ggcggaatac tgcaaccgcg tttccatcat ggtggacggc gtgatcgcgg
      721 cgctggacag cccggcgacg ctgaaaaaac agttcggtgg tgcgagcatg gaagacgtat
      781 tctatgaatt ggcacgtaac gctaaaagaa agggagacta acatgcgaca actaatcatc
      841 ttcatccgaa aggaattcct gcatgtcttc cgcgaccgga agacccttct gatgctgttc
      901 ggtctaccga tcgcgcagat cgtactgttc ggcttcgcac tatccaacga gatcaaggac
      961 tcgaacatcg cgctcgtgga ttattccaaa gacgcggtga cgacccggct catcgggaag
     1021 atcgaggcca gccattactt cgacatccgg cgtacgctgg cggacgccac acagcttcag
     1081 gccgccttta aaaaagggga catcaaaatg gccgtgatct ttcccgccaa tttcggaaac
     1141 gatctgatgc ataccggcaa ggccacactc caggtcatcg ccgatgcttc cgaccccaac
     1201 caggcgacga ccatcaccaa ctatctccag caggtggtcg ccgactataa taacgagctc
     1261 gccgccggtg cgcaacaacc gctccgcatc gtcccgcaga tgcagatgct atacaacccc
     1321 gagctccgcg gcgcccccaa tttcgtaccc ggcgtcatgg cgctggtgct gatgctcgtg
     1381 tgtaccatga tgacggcggt ggctatcgta aaagagaaag aattaggtac gatggaggtc
     1441 ctgctggtct cgccgttcaa accggtgtat atcatcatct ccaagctgat gcccaatctc
     1501 atcatttctg tggtcaacct ggtcatcatc ctcgtgctca gcaacgtttt cctcgatctg
     1561 ccaatccggg gcagcaccct ccttctcatt ttctgcagta ttctttttat cctcacctgt
     1621 ctttcgatcg ggctcctggt gtccatcggc gccaagaccc aacagaccgc gatgatgggc
     1681 tcgatgatgg gcatgatgct gccgaccatg ctgctcaccg gattcctgtt cccgatcgag
     1741 aatatgccgg tagtcctgca ggtactctcg aaccttgtcc cgtcccgatg gtattttatc
     1801 atcatcaaac gcgtcatgct gaagggcctc ggtttcggcg ccatctggaa ggagaccctc
     1861 atcctggcag gcatgtgcgt cttcctgttc accatcagtc ttcgcaactt taaaatcaga
     1921 ttgcaatgag gaccttacgc ttcttactcg aaaaagagtt ccgccaattg cttcgcaacc
     1981 gacagctggt ccgcaccctg ctgctggccc cgctgatcca gctgatcctg ctcccgatgg
     2041 cggccgacta taccgtaaaa aatattcaca tcgtggtggc ggacaacgac cattcgccgt
     2101 cctcgcgccg tctcatcgcc aagatcacct cttccggata cttccatctt tccggttaca
     2161 ccgcctccta tcccgaggcg cttcgtatgg tcgagcagga caaggccgat ctggccttac
     2221 agatccccgc gcatttcgaa cgcgacctgg tacgcgaaaa tgccaaccag ctgtatatcg
     2281 ccatcaattc catcgacggc accaaaggca acctgggagg cgcctatctg cgcagtatca
     2341 tcaccgattt caacgacgat ctacgcgtcg atatgaagac cgtggagccc gttcaggaag
     2401 accctccgca ggtgtcggtg gcctcttcca actggtacaa tcctttcctg aaattccctc
     2461 tctatatggt ccctgccatc ctcgtgacgc tcgtcacagc gatggtggcc atgcagacgg
     2521 ctttcaatat cgtccaggaa aaagaaagcg gcaccatcga gcagatcaat gtgacaccca
     2581 tcaaaaaata catcttcatc accgggaaaa tgatcccttt cctggtgctc ggtatcatcc
     2641 ttttcacgat ggggctgctg gtcggctggg cgctttatgg agtcgttccc ctgggcaatc
     2701 tctgggtcct gtacggttcg ctgatcgtct atctcctgtc catgcttggg ctgggactgc
     2761 tgatcgccac ctattccggc acgcagatgc aggccatgtc gctggccttc ttcttcatca
     2821 acatcttcaa tatgatgagc ggggtattca cggcagtcga cagcatgccg gcctgggcgc
     2881 aggccatcgt ggctacattc ccgcccagcc attttatcag gatcatgcga atggtagtgc
     2941 tgaagggcag cgggttcggc gacatccgtt atgagctggt ctggatgctg gtcatcgggc
     3001 tgttcctcaa tgcctgggcg ataaaaaatt acaggaaaat cgcctagccg gccttgggcc
     3061 ggcccacgcg acgcatgcct acagcgacac cccgcgtttc cacgggataa aatcatcctg
     3121 cacgtgtctc accgcagcca cctgcgtctc gccgctggcg acaccaataa catattcgag
     3181 gatgcgctcg ccggcctctt tgatcgtctc attcccctcg atgatcgtgc ctgtattgat
     3241 atcgatgata tcccgcatcc tatcgaaaag cgcggtattg ctcgcaatct tcacaacagg
     3301 ggctatcggg ttaccggtag gcgtgccgag cccggtcgtg aacaatacga tattggcccc
     3361 cgacccgact tccgcagtgg tcgattcaac gtcattaccc ggcgtgcaca acagattgag
     3421 accgggttta gacactttct cgggataatc gagaacgtcg gacaccggtg acgtgccccc
     3481 tttcttggcg gctccggcgg atttgatggc gtcggtgatg agaccgtcgc gtatattgcc
     3541 cggcgatgga ttcatatcga aacccgatcc caccgctttg gcgcggtcat tataggtgcg
     3601 catcaaatgc gagaaccttt cggccgtagg aacatccata caacgatcgc tgaggttctg
     3661 ttctacaccg cacaattccg ggaattcgga cagaatcacc gagccaccca tcgctaccag
     3721 cagatcggat gtataaccga tcgccgggtt ggccgatatt cccgagaaac catccgatcc
     3781 gccgcattcc aatccgatac agagcttact caaaggcgcg ggtcggcgct cctgctgatt
     3841 ggcaaggata agccccgcga aagtttgttt tatggcttcc gaaaggaatg ccgattcggt
     3901 acctatcttt tgctgctcga ggatatacat cggtttcgaa aattcggggc ttcgcttcct
     3961 gatctcctcc tcgagtatgg aaacctgcgc attttgacaa ccgaggctta atacagtagc
     4021 ccccgcgaca ttcggatgcg taatatagcc cgccagcaac ccgcataagg cctgtgcatc
     4081 cgccctcgtt ccaccgcaac caccgtcatg attcaggaat ttaataccat ccacattcgg
     4141 aaataaacgc tcgctcgcat tctgctcata atccgcctga aggtccgtcc ttaggatatc
     4201 ctccaccgtc ttgcctgccc tgtatagctc gaccagctgc tgc
//
LOCUS       DASYSV010000596         8717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_891904, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000596 DASYSV010000000
VERSION     DASYSV010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891904"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VGQ51_18545"
     CDS             complement(<1..675)
                     /locus_tag="VGQ51_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEV9038646.1"
                     /translation="MTNSKPDLHTALSPALLHLYDVNHAIVVIIDVLRATSTIATALY
                     NGAKCVIPVDSVAKCIELGRQIDGITAGERDGMIAEGLQHGNSPFEYPREFVGGKTLV
                     LTTTNGTKLLHMALDRGAGQIVTGSFPNLDAVCRYLVAQNQPVVLGCAAWKDRVNLED
                     MLFCGAVIHEVKSHFSINCDSSQVAETVYLQAEKDLFGFMKSKDASHYHRLSGYGLEK
                     DIRYCLT"
     gene            complement(775..1251)
                     /locus_tag="VGQ51_18550"
     CDS             complement(775..1251)
                     /locus_tag="VGQ51_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV9038647.1"
                     /translation="MPTIHLTTFIAAPADRVFDLARSIDLHRKSMAHTDEQPIAGVTS
                     GLIGLDETVTWKAKHLMKTRILKSRISAMNRPFSFTDEMVSGDFRSLRHEHHFKPTGN
                     GTLMIDLMHFESPYGSLGRLINNIFLTRYLRGLLEMRNHQLKEYAESEKWKFILEK"
     gene            complement(1345..2430)
                     /gene="gcvT"
                     /locus_tag="VGQ51_18555"
     CDS             complement(1345..2430)
                     /gene="gcvT"
                     /locus_tag="VGQ51_18555"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEV9038648.1"
                     /translation="MKHTPFTSMHIGLGAKMAEFAGYNMPISYSGINDEHLAVRTNAG
                     VFDVSHMGEFIVKGEHALDLIQRVTTNDASKLSAGKAQYSALTNDKGGIVDDLIVYCL
                     EENKAYMLVVNASNIEKDWKWISAHNTAKAEMHNISDKTCLLAIQGPNATKILQPLTG
                     IDILNLSYYTFAKGTFAGVDNVLISATGYTGAGGIEIYFEDKDGAAETIWNKIFGIGS
                     PQGLKPIGLGARDTLRLEMGYCLYGNDIDDTTSPLEAGLGWITKFAKPFTAREILEKQ
                     KAAGPAQKMVGFVMEEKGIPRHDYEIKDFSGTTIGRVTSGTQSPSLGKAIGMGYVRPA
                     FSQLDTKVYIKVRDKLLQARVVKFPFS"
     gene            complement(2576..4813)
                     /locus_tag="VGQ51_18560"
     CDS             complement(2576..4813)
                     /locus_tag="VGQ51_18560"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV9038649.1"
                     /translation="MKKIININFHSRVIPIEESAYEILQQYIGSLRRYFAHEEGRDEI
                     ISDIENRFAELFSDTLKKGAPCITDADVEANIASMGRPEEFEGEENAGAAGPSTGTST
                     AAGSAQNTGTGANPNWQFAGEPRRLYRADNDKILGGVCAGLANYLRLDPAIVRIIFVL
                     MLFGGGFGFLLYMVLWVVLPTRSLPAAVRKRLYRNPDDRVIAGVASGLAAYFHIEVWI
                     PRLVFVLPFALGIVAAIFRGFWWHWGDHFNGGGFFAGGFGGSLIISYIVLWIVLPEAN
                     SASEKLEMRGEKVDLESIKNTIKSDLEGFKGRAKEMGTEMGQRFQQVGKEMKQSTQNW
                     ASEAAPTIRQTSNSFGHAIGVLFKAFFLFIAGCIAFALIMALAALVFRGNGVLHVKNF
                     ILEGFWQNFLAWAGFFLFLIIPVIALLTWLIRRISGTRTRRHYLGYAFMTLWVIGLFC
                     IIALSGMVMNNFQSRQHAEETFPLMQPTHGKLLVNANYDENRYGDTDEWFNVDWGRNN
                     AFHILNDDSVLLRTVRVNVLKSDDSAFHVQLIKLSHGNSGANARELATDIRFPVNQND
                     SILTLPRGFTITPGEKFRNQQVLVQIYVPVGRRIQLSRSVKDYNYFNIDPRRRHISWN
                     DGHRYYEWDEDLDEGNTYNWNSNTEYVMTPGGLVRTDRNTDEDNQKLDRRPNHKNNDN
                     ADEDEPGYSSPKSARPNNGGYRYKGPGSQPEKKTTDSTPAKRTAMTAKPADCSTCLLS
                     ALDNI"
     gene            complement(4865..5197)
                     /locus_tag="VGQ51_18565"
     CDS             complement(4865..5197)
                     /locus_tag="VGQ51_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV9038650.1"
                     /translation="MNIENTQSQMRKGILEFCILSVIRRGEAYPSDIVEEMKNANLNI
                     LEGTLYPLLTRLKNADMLTYRWVESNSGPPRKYFSLTPKGEEFYGELEATWNELANAV
                     HALTNNTH"
     gene            complement(5491..6432)
                     /locus_tag="VGQ51_18570"
     CDS             complement(5491..6432)
                     /locus_tag="VGQ51_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEV9038651.1"
                     /translation="MAIPVVDLSEFSGAPSQKATFVSALGKAYEEVGFVAVKNHGIPT
                     STIDGLYSNTKAFFGLPHETKRKYEIAGLAGQRGFTSFGKEHAKGSKAPDLKEFFQFG
                     QTVEDHDPIKAEYPDNVQVKELPEFNPLFVRAYRSFERSGTALLQAIAIYLGLDEHYF
                     DAYVHNGNSILRAIYYPPIKEEPKSAIRAEQHEDINLITLLVGASADGLQILSKQNEW
                     VPVTSLPDQIVVNVGDMLQRLTNNRLKSTTHRVVNPPKELWGTARFSIPFFLHPKSSM
                     SLACLPSCIDSRHPKAYPDATAGEYLDERLREIGLKK"
     gene            complement(6463..7902)
                     /gene="asnS"
                     /locus_tag="VGQ51_18575"
     CDS             complement(6463..7902)
                     /gene="asnS"
                     /locus_tag="VGQ51_18575"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV9038652.1"
                     /translation="MFNTRVKVKALLAATPDEREITVMGWVRSFRNNQFIALNDGSTN
                     SNLQVVAELGVFDESLLRKITPGASIKVTGQLIASLGKGQTVEVKARTIEILGECNPD
                     TYPLQLKNRPSLEYLRDIAHLRFRTNTFAAIFRIRHALSFAIHRFFNERGFVYLHTPI
                     ITASDAEGAGEMFHVTTLPFDSTPRKEDGSVDFAEDFFGRATNLTVSGQLEGELGAMA
                     LSEIYTFGPTFRAENSNTARHLAEFWMIEPEMAFYDLEDNMNLAEAFIQYLIRYVMEN
                     NREDLEFLAQRQAEEEKQKPQAERSEFGLIEKLEFVLNNDFERITYTEAIDILLQSPA
                     YKKKKFQYEVKWGIDMQSEHERYLVEKHFKKPVIVTGYPAAIKAFYMRRNEDGKTVAA
                     MDILVPGIGELVGGSQREERLEKLEERMKEMHVPIEEMWWYLDTRRFGTVPHAGFGLG
                     FERMMLFITGMTNIRDVIAFPRAPKSAEF"
     gene            8158..>8717
                     /locus_tag="VGQ51_18580"
     CDS             8158..>8717
                     /locus_tag="VGQ51_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEV9038653.1"
                     /translation="MYDILQLNDMLVPELLDIAEQLKIAGAKKLDKQELIYKILDKQA
                     VVASEGKGETADDKPKRKRIIKTTTGNSTEEAIVESGEDNKPKKADPKKETRNKKEEK
                     PMLKKPRKKEEEEPVEQMAASKAAPVAKVHPAAHRDASRGDASRGDASRGDAPQRDAP
                     QRDASYNRDAPQRDASRREGMHTGEA"
BASE COUNT         1967 a         2235 c         2376 g         2139 t
ORIGIN      
        1 cgttagacaa tatcttatat ccttctccaa cccatacccc gagagccgat gatagtgcga
       61 cgcatccttc gacttcatga acccgaacag gtctttctca gcctggaggt ataccgtttc
      121 ggcgacctgc gaggaatcgc aattgatgga gaaatggctt ttcacctcgt ggatgacggc
      181 gccgcaaaac agcatgtcct cgaggttcac gcggtccttc caggccgcgc accccagtac
      241 tacgggttgg ttttgggcaa cgagatagcg gcagaccgca tccagattgg gaaatgaacc
      301 cgttacgatc tgacccgcac cgcggtcgag ggccatatgc agcagcttgg tgccgttggt
      361 ggttgtgagg acaagggtct tgcctccgac gaattcccgg gggtattcga agggtgagtt
      421 gccgtgctga agaccttcgg cgatcatgcc atctctttcc ccggccgtta tgccgtcgat
      481 ctggcgaccg agctcgatac atttggcgac gctgtccacg gggatcacgc atttggcgcc
      541 attgtacagt gcggtggcga tggtagaggt tgcacggagg acatcgatga tcactacgat
      601 ggcatggttt acatcgtata aatggagcaa ggcaggagac aatgccgtat gaagatcagg
      661 tttggaattg gtcataaagg gaaacgtggg atcagttagc gcgaaaatca ctgccggagg
      721 attttcgcga ggcgtaaaga tagggcgttc gtggcaatgt aacggggacc gaagctattt
      781 ctcgaggatg aatttccatt tttcagattc ggcatactcc tttagttgat ggttacgcat
      841 ctcgagcagg cccctgagat aacgggtaag aaagatgttg tttatcagcc ggccgagact
      901 tccataaggg ctttcgaaat gcatcagatc gatcatcagg gtgccgttcc cggtcggctt
      961 gaaatgatgt tcatgccgaa ggctcctgaa atccccgctc accatttcgt cagtgaaaga
     1021 aaaaggacgg ttcatggcgc tgatcctcga tttcaggatc cgggtcttca tcaggtgttt
     1081 tgccttccag gttaccgttt catccagtcc tatcaaaccc gaggtgacgc cggcgatcgg
     1141 ctgttcgtcg gtatgcgcca tggatttacg atgtaagtct atgctccggg ccagatcgaa
     1201 gaccctgtcc gcgggggcag cgataaaagt ggtaagatgg atggtaggca ttacttaggt
     1261 gttggatgcc ttgattgtaa gaaaaaatca ttccaaagcg ttggcgggga agcaaagcgc
     1321 gcggttgccg cccgtctttt tcgatcagct aaagggaaac tttaccacgc gggcctgcaa
     1381 aagcttgtcc cttaccttga tgtagacctt ggtatccaac tgggaaaacg ccggacggac
     1441 atagcccatc ccgatggcct ttccgagcga cgggctctgc gtgcccgagg tcacacgtcc
     1501 gatcgtcgtt ccggaaaaat ccttgatctc gtagtcatgc cgggggatgc ctttctcctc
     1561 catcacgaac ccgaccattt tttgcgccgg accggcagcc ttttgttttt cgaggatctc
     1621 ccgggcggtg aagggttttg caaacttggt gatccagccc agacctgctt cgagcgggct
     1681 ggtagtatcg tcgatatcgt tcccgtacaa acaatagccc atttccagac gcagggtatc
     1741 ccgggcgccg agacctatgg gtttcagacc ttgaggactt ccgatcccga aaatcttgtt
     1801 ccagatggtc tctgcggctc cgtccttatc ttcaaaatag atctcgatac ctcccgcccc
     1861 ggtataacca gtggcgctga tcaggacatt gtcgaccccg gcgaacgttc cttttgcaaa
     1921 tgtataatag gagagattga ggatatcgat cccggtcagc ggctggagga tcttagtggc
     1981 attgggtccc tggatcgcca gcagacaggt cttgtcggaa atattgtgca tttccgcctt
     2041 agcggtattg tgcgcggata tccatttcca atccttttca atattggacg cattgactac
     2101 cagcatatag gccttgttct cttcgagaca atacacgatc aggtcatcca cgatgcctcc
     2161 cttgtcattg gtcagcgcgc tgtactgggc tttcccggcg ctcagcttcg aagcgtcatt
     2221 ggtggtcacc cgttggatga gatcgagagc atgctcaccc ttgacgatga actcccccat
     2281 atggctgacg tcgaagacgc cggcattggt tcgtaccgca agatgctcgt cattgatacc
     2341 gctatagctg atgggcatat tgtagccggc gaattccgcc attttggctc cgagcccgat
     2401 atgcatcgat gtaaaaggag tgtgcttcat gtattgactt ttcagcgggg gcaaatatag
     2461 gaaaaaaagc cccccggcag aggccgggcg gctatgttcc aacttcaacc aacgaagatc
     2521 atggatagac cggggtatcg gcgacacgaa cgcatcgtct tcagggatga accggctaaa
     2581 tattatcgag ggcggagagc aggcatgtgc tgcagtctgc cggttttgcc gtcatcgccg
     2641 ttcttttggc gggtgtcgag tcggtcgttt tcttttcggg ttgggacccg gggcccttgt
     2701 agcggtatcc gccgttattt ggcctggcag atttcgggct gctgtagccg ggctcgtctt
     2761 cgtcggcatt gtcattgttc ttatggttgg gccggcgatc cagcttttga ttgtcctcat
     2821 ccgtattccg gtctgtccgt accagaccgc ccggggtcat cacatattcg gtattgctgt
     2881 tccaattgta ggtgttccct tcatcgagat cttcgtccca ttcgtaatac cggtgaccgt
     2941 cattccagga aatgtgccgg cggcgcgggt cgatattgaa atagttatag tcttttaccg
     3001 acctgctgag ctggatacgc cgccctacag ggacatagat ctgaaccaat acctgctggt
     3061 tgcggaactt ctcacccgga gtgatggtga agcccctcgg cagcgtaagg atactgtcat
     3121 tttgattgac ggggaaccgg atgtcggtgg caagctcccg ggcattggcg ccgctgttgc
     3181 catgactcag cttgatcagc tggacatgaa aggcggaatc gtcgcttttc aacacattca
     3241 cccgcactgt cctcaacaat acgctgtcgt cgttaaggat atgaaaggcg ttgttgcgtc
     3301 cccagtcgac attgaaccat tcatcggtat cgccataacg gttctcatca tagttcgcgt
     3361 tgaccagcag cttgccatgg gtcggctgca ttagcgggaa agtttcttcc gcatgttgac
     3421 gggactgaaa attattcatg accattccgc tcagggcgat gatacaaaaa agaccaatga
     3481 cccacagagt catgaatgca taacccaggt aatgtctgcg cgtacgggtc ccggagatgc
     3541 ggcggatcag ccatgtgagg agggcgatca ccggtatgat caggaacagg aagaacccgg
     3601 cccaggccag gaagttctgc cagaaccctt ccaggataaa attcttcaca tggagtaccc
     3661 catttccgcg gaagaccagc gctgcgagcg ccatgatcag cgcgaaggcg atacagcctg
     3721 cgatgaagag aaaaaaggct ttgaacaata cccctatggc atgcccgaag ctattgctgg
     3781 tctgccggat ggtaggagcc gcttccgatg cccagttctg ggtgctctgt ttcatttcct
     3841 tacccacctg ctgaaaacgt tggcccattt cggtgcccat ttctttggcc ctgcccttga
     3901 agccttcgag atcgcttttg atggtattct taatggactc cagatccact ttttcaccgc
     3961 gcatctcgag cttctccgag gccgaattgg cctcgggaag cactatccat aagacgatat
     4021 agctgatgat gagcgatccg ccgaacccgc cggcgaaaaa gccgcctccg ttgaaatgat
     4081 ccccccaatg ccaccagaat ccgcggaata tcgcggccac gatgcccagc gcgaacggca
     4141 ggacgaagac gagccggggg atccagacct cgatatggaa ataggcggcc agaccactgg
     4201 caacaccggc gatgacgcga tcatccgggt tgcggtacag acgtttgcgg acggcggccg
     4261 gcagcgatcg ggtagggagg acgacccaga ggaccatata gaggagaaaa ccgaaaccac
     4321 cgccgaaaag catgagtacg aagatgatcc ggacgatggc ggggtccagc cggagatagt
     4381 tggcaagacc ggcgcatacg ccgccgagga tcttatcgtt gtcggcgcga tagagtctgc
     4441 gcggctcgcc ggcgaattgc cagttggggt tggcgcctgt gcccgtattc tgggcactgc
     4501 ctgcggccgt tgaagtcccg gttgagggcc ctgcggcgcc tgcattctct tccccctcga
     4561 attcttcggg gcgtcccatg ctggcaatgt tggcttcgac atcggcatcg gtgatgcagg
     4621 gcgctccttt tttcagggta tcggagaaca gttccgcgaa gcggttctcg atatcgctga
     4681 tgatctcgtc cctcccctcc tcatgcgcaa agtacctgcg gagacttccg atgtattgtt
     4741 gcaagatctc ataggcggac tcttcgatag ggatcacccg gctatggaaa ttgatattaa
     4801 tgatcttttt cattgtaagt gtatttggag gttgaaatgg tgtctttcgt gcattcaaag
     4861 gttgctaatg ggtgttattc gttagggcat gtacggcatt ggccagttcg ttccaggtgg
     4921 cttccagctc accgtaaaac tcttcccctt ttggcgtgag ggagaaatat ttgcgtggag
     4981 ggccggaatt gctttccacc caacggtagg tcagcatgtc tgcatttttg agtcgtgtca
     5041 gcaggggata aagggttcct tccaggatgt tcaggttggc gttcttcatt tcttctacga
     5101 tgtccgaagg gtacgcctca ccgcgcctga ttacagagag gatgcagaac tccaggatcc
     5161 ctttgcgcat ctgactctga gtattttcga tattcatacg ctgagttttt tacttttccc
     5221 gtccccccgg cgccgatcgg taacaccgga aaaacaggtt aattatcctt aacaaatcaa
     5281 agatatgtgc ttttctagta ctatgcaaca cacagtacta ttttaaagaa ggtatttttt
     5341 atcgtcagga aggcagaaca ggcctggaat atagcatgga cgtgtctttc cgccatggac
     5401 cgagccgcgg gaatttcttt tgatgattgg ataaaaggtg gaaaaacggg gtgagcggcg
     5461 gtcaagacct ggcacggggc ggtcctcgcc ctatttcttt agtccgatct cacgcaaacg
     5521 ctcatcgagg tattccccgg cggtagcgtc cggataggcc tttggatgtc gtgaatcgat
     5581 acaggagggc agacaggcca ggctcatgct gcttttagga tgcaggaaaa agggaatgga
     5641 gaaacgcgcg gtcccccaga gctcctttgg aggattgaca actcgatggg tggtcgattt
     5701 gagccggttg ttggtcaacc gctgaagcat atctcccaca ttcacaacga tttgatccgg
     5761 gagcgaggtt acgggcaccc attcgttctg tttggaaagg atctgaagcc catcggcgct
     5821 ggctccaact aacagcgtta tcaggttgat atcctcatgc tgttcggccc ggatggccga
     5881 tttcggctcc tcttttatcg gcggataata gatcgccctc aaaatggaat tgccattgtg
     5941 gacatacgca tcgaaatagt gttcgtcgag acccagatag atggcgatgg cctgcagaag
     6001 ggcggtgccc gaccgctcga aagaacgata ggcccggacg aaaagaggat tgaattccgg
     6061 cagctctttt acctgcacat tgtcgggata ttccgccttt atcggatcat ggtcttcaac
     6121 cgtctgtcca aactgaaaaa actctttcag gtccggagcc ttgctgccct tggcatgttc
     6181 ctttccgaaa gaagtaaagc cgcgctggcc ggcgagaccg gcgatctcat attttctttt
     6241 ggtctcatgt ggcaatccga aaaaggcctt cgtattcgaa tacagaccgt cgatggtcga
     6301 ggtggggatg ccatggttct ttactgcgac gaagcccact tcttcgtagg cttttccgag
     6361 ggcggaaacg aatgttgctt tttgagaagg ggcgcctgag aattcggaaa gatcgacgac
     6421 tggaatggcc attgtaaagg tttgaataaa ggtaggcttt tcctagaact ccgcgctctt
     6481 aggcgcccgc gggaaggcga tcacgtcgcg gatattcgtc atgccggtga tgaacagcat
     6541 catccgctcg aaccccagac cgaatcctgc atgaggcacc gttccgaaac gacgcgtatc
     6601 gagataccac cacatctctt cgatcggaac atgcatttcc ttcattcttt cctccagctt
     6661 ttcgagcctc tcctcgcgct gagagccgcc gaccagctca ccgatgccgg gcacaaggat
     6721 gtccatggcg gcgacggtct ttccatcctc attgcggcgc atatagaaag cctttatggc
     6781 ggcgggatag ccggtgacga tgacgggttt tttaaaatgt ttttccacga gataccgttc
     6841 gtgctcgctt tgcatgtcga taccccattt tacttcgtac tggaattttt tctttttgta
     6901 agcgggcgat tgaagaagaa tgtcgatggc ctcggtatag gtgatccgtt cgaaatcgtt
     6961 attcaggacg aattccagtt tttcgatcaa tccgaactcg cttctctccg cctgcggttt
     7021 ctgtttttct tcttccgcct gacgctgcgc caggaattcg agatcttccc ggttgttctc
     7081 cattacatat cggatcaggt attggatgaa agcctcggcc aggttcatat tgtcttcgag
     7141 atcgtaaaag gccatttccg gctcgatcat ccagaattcg gcgaggtgac gcgcggtgtt
     7201 cgagttctcg gcccggaacg tgggtccgaa ggtatagatc tcgctaagag ccatggcgcc
     7261 gagctctcct tcgagctgcc cgctcactgt caggttggtg gcgcggccga agaagtcttc
     7321 cgcgaaatcg acggacccat cctccttacg cggtgtactg tcgaaaggca gggtggtcac
     7381 atggaacatc tctcccgcgc cttcggcatc cgacgccgtg atgatcgggg tatgcagata
     7441 aacgaaaccc ctttcgttga aaaaccggtg gatcgcgaac gagagggcat gccggatccg
     7501 aaagatggca gcaaatgtat tggtacggaa tcggagatgc gctatgtcac gcagatattc
     7561 gagactgggt ctgttcttca gctgaagagg ataggtatcg ggattgcatt cgcccaggat
     7621 ctcgatcgtc cgcgctttta cctccacggt ctggcccttg cccagcgagg cgatcagctg
     7681 tcccgtcact tttatcgacg ctccgggggt tatcttccgc agtaaagact catcgaaaac
     7741 acccaattcg gccactacct gcaggttcga attggtcgat ccgtcattca gggcaatgaa
     7801 ttggttgttg cggaagctgc gcacccatcc catcacggta atttcgcgtt catccggggt
     7861 ggcggccagc agcgccttca ctttcactct tgtgttgaac atggtttgcc tgattaaatt
     7921 tcgtggtgca aaattgccga aaatcgactg aaactcaggt gctttaaaaa tttttttttg
     7981 aattatgcat ttttatgcta aaattgcagt ggaattaagc tgatcagttt ccctttccaa
     8041 tgctcacagc ttatcagttt taaactttct gttcatataa ttcaataatt catttcaaac
     8101 attttcaact agacagtctt actgtttgag aatgctgttc aactctcaag ttattttatg
     8161 tacgatattc tccaattgaa cgacatgctc gttccggaac tcctcgatat agctgaacaa
     8221 ttgaagattg ctggtgcaaa aaaactggac aagcaggaac tcatttacaa gatactcgat
     8281 aaacaagccg ttgtcgcaag cgaaggaaaa ggcgagaccg ccgatgacaa gcccaaacgc
     8341 aaacgcatca taaagaccac taccggtaac agtaccgaag aagccattgt agaaagcggc
     8401 gaggataata aacctaaaaa agccgatcct aaaaaagaaa cgcgcaacaa gaaggaagaa
     8461 aagccgatgc tgaagaagcc gcgcaaaaaa gaagaagaag agccggtgga acagatggct
     8521 gcgtcaaaag ccgcgcctgt cgctaaagtt catccggccg cgcatcgcga tgcgtcgcgt
     8581 ggggatgcgt cgcgtgggga tgcgtcgcgg ggggatgccc ctcagcggga tgcccctcag
     8641 cgggatgcat cgtataacag ggacgcgccg caaagggatg cctcgcgtcg cgaaggtatg
     8701 catacgggcg aggcgca
//
LOCUS       DASYSV010000597         7562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_377903, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000597 DASYSV010000000
VERSION     DASYSV010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377903"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VGQ51_18585"
     CDS             <1..1396
                     /locus_tag="VGQ51_18585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV9038654.1"
                     /translation="KGRTLMETIIPTSYDHSAWTGFQGFLESEGVSILNQRLELTAVR
                     KSKEEFPIELTVTAIDDESESYYSAFIRDISSRKQREQELVQMKEKAEQAAKAKSQFL
                     SVMSHELRTPLNAVIGITHLLLQSQPREDQQEDLRTLQFSGESLLHIINDILDFTKLD
                     SGKIELSSIDFNLRELTQSLYQSFSFKAKEKGIVFDVEYDERMPFYVKGDSFRLSQVL
                     NNLISNAIKFTLEGFVKLKVEMAERQGATYVTRFSVIDSGIGIPEDKLDKIFEQFTQA
                     DSDTTRLYGGTGLGLSISSRLVELMGSSILVASSPGKGSDFHFTLPLLQGHQTDAATA
                     TTKIVPKSNEQFKNKTILLAEDNVFNANIARRFITGWGAQLEIVVDGRQALEFVSRRK
                     YDLILMDVQMPVLDGFACTRKIRKHFKDIPIIAITASPKNEIIHEIMSCGMNDFVSKP
                     FKPNELRTKLLEWL"
     gene            complement(1424..1924)
                     /locus_tag="VGQ51_18590"
     CDS             complement(1424..1924)
                     /locus_tag="VGQ51_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV9038655.1"
                     /translation="MLKIGLIADTHHFLDENVFRHFETCDEVWHAGDFGTIGLARRLS
                     DFKPLKGVYGNIDGPDIRSVYPEKLRWQSEGVHVFMTHIGGSPPRYNPGVRKELQTAT
                     PQLFICGHSHILKVIYDEKIHCLHMNPGAAGRQGWHTVRTIIRLTIDGKDMRDCEVIE
                     LGPRGG"
     gene            1967..3124
                     /locus_tag="VGQ51_18595"
     CDS             1967..3124
                     /locus_tag="VGQ51_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEV9038656.1"
                     /translation="MPHYHSLGTIPHKRHTQFRQGDGSLYSEQLFSTEGFSNDYSLLY
                     HIHPPTEIIATDAPYDAQPRIAEEKMLKHRSFDGFLVKPEADFLQSRKAVLVNNDCHI
                     VLAAPAAGMENYFYKNADADELIFVHHGGGTLKTMYGELSFGYGDYLLVPRGTIYQIQ
                     FDGPDNRLFIVESFSPIRYPKKYLSPYGQLLEHSPYCERDIRGPQSLSPRDEKGDFLI
                     QTKKKGRVYGIHYGHHPFDVVGWDGYCYPFAFSIHDFEPITGRVHQPPPVHQTFEGHN
                     FVVCSFVPRLFDYHPMSIPAPYNHSNIDSDEVIYYVDGDFMSRKNVTRGMITLHPGGI
                     PHGPHPGAVARSIGAKETKELAVMVDTFHPLMLTVEALGIENQGYTMSWAE"
     gene            3151..4797
                     /locus_tag="VGQ51_18600"
     CDS             3151..4797
                     /locus_tag="VGQ51_18600"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV9038657.1"
                     /translation="MKHTRLAAAAAIVLFSCRGKQPADLIVYHAKIYTVDSAFDTVQA
                     MAVKDGRILATGSDEDVLARYSAPAMTDEGGKAVYPGFIDAHSHFISYGESLFMTDLF
                     GCTSTEEMVQRVKQFTDAHPGLAWIRGRGWDQNKFPGKAFPDNTLLNQLFPNTPVVLG
                     RVDGHALLANAKALELAGVHAGQQIGGGVIETKNGRLTGIMIDNAQRLVYAHEPDLSP
                     AEFLERVDAAQQKCFAQGLTTVADCGIDRLAIEAIDSLQKTGKLKMRVYALLSDRPYN
                     LDHYLPRGPYKTDRLYVGGVKAYADGALGSRGACLLAPYSDKPGWVGFLLSPPAHFDS
                     LAARLAPTDWQLCTHAIGDSGNRMILQVYNKYLKGKNDRRWRVEHAQVIDEADFDLFG
                     KASIVPSVQPTHATSDMYWAGERLGPQRLKNAYAFKRLLQQNGWVALGTDFPVEDISP
                     FKTFLAAVFRVDAKGYPSGGFQPENALSREETIRGMTIWAARADRWEDRIGSLEAGKV
                     ADFVVLDKDMMQVPAGEVLSVKPVATYSSGMQVFGAGGSK"
     gene            4857..5858
                     /locus_tag="VGQ51_18605"
     CDS             4857..5858
                     /locus_tag="VGQ51_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV9038658.1"
                     /translation="MEYRHLGESGVKASAITFGAWAIGGWLWGGSDRKDAVAAIRASY
                     DEGVTSIDTAAVYGMGLSEEIVGEALQGIPRDSVQIMTKFGMRWDMTRGDFGFKSQDN
                     EGRPLDIYKYGGKESVIRECEDSLRRLRTDYIDLYQQHWPDITTPISETMEALDELMT
                     AGKIRAAGVCNYDTAQMAEADKTIRLASDQVPYSMVRRDIEDELVPYCLSHKKAILAY
                     SPLQRGLLTGKIRPGHRFNEGDNRGDSKYFRGENLTRINAFLDELRPMAEERGASLTQ
                     LVIRWTLERPGITIALVGARDARQAVENARAMQVRLSEDEMAVIDEKLAELQGALVM"
     gene            6007..6420
                     /locus_tag="VGQ51_18610"
     CDS             6007..6420
                     /locus_tag="VGQ51_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038659.1"
                     /translation="MVPFHELRIGDIVLIEFNGQRTEGEVIDKADADRLVCVDNEAQA
                     FWYGSEELYPIPLDENQLLKFGFEKQVQPDGSVKYLRGPFRILLHVPGSFAHFEMWYR
                     EDRRHIIEHISVHQLQNHYHQMTKVDLVRGVGAHH"
     gene            6655..6873
                     /gene="infA"
                     /locus_tag="VGQ51_18615"
     CDS             6655..6873
                     /gene="infA"
                     /locus_tag="VGQ51_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV9038660.1"
                     /translation="MAKQALIKQDGIILEALSNAMFKVKLENGHEILATISGKMRMNY
                     IRILPGDKVGVEMSPYDLTRGRIIFRYK"
     gene            6940..7056
                     /gene="ykgO"
                     /locus_tag="VGQ51_18620"
     CDS             6940..7056
                     /gene="ykgO"
                     /locus_tag="VGQ51_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEV9038661.1"
                     /translation="MKVRASIKKRSADCKIVRRKGKLYVINKKNPRFKQRQG"
     gene            7085..7465
                     /gene="rpsM"
                     /locus_tag="VGQ51_18625"
     CDS             7085..7465
                     /gene="rpsM"
                     /locus_tag="VGQ51_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV9038662.1"
                     /translation="MARIAGVDLPKNKRGEIGLTYIFGIGRSTAQYILTKAGIEFDKK
                     VHQWDDNELNAIRNIITNEFKVEGALRSEVQMNIKRLLDIACYRGLRHRKGLPVRGQR
                     TRTNSRTRKGKRKTVAGKKKVAKK"
BASE COUNT         1799 a         1667 c         2399 g         1697 t
ORIGIN      
        1 aaagggtcgt acgctgatgg agacgatcat cccgacgtcc tacgatcatt ccgcatggac
       61 cggattccag gggttcctgg aaagcgaagg ggtatccatc ctcaatcagc ggctggagct
      121 gacggccgtc cggaagagca aggaagaatt tcccatcgag ctaacggtta cggctatcga
      181 cgacgagagc gaatcctatt acagcgcatt tatccgggat atttcaagcc gcaagcaacg
      241 tgagcaggaa ctggtgcaga tgaaggagaa ggccgagcag gcggcgaaag cgaaatccca
      301 gttcctgagc gtaatgagcc atgaattgag gacgccgttg aatgcggtga tcggcatcac
      361 gcatctgctg ttgcagagtc agccccggga agatcagcag gaagacctcc ggaccctgca
      421 gttttcgggc gagagccttt tgcatatcat caacgatata ttggatttca ccaagctgga
      481 ctcggggaag atcgagcttt cttccatcga tttcaatctg cgggaactca cacagagcct
      541 ttatcagtcc tttagcttca aggccaagga aaaggggatc gtcttcgacg tggaatatga
      601 cgagcggatg cccttttacg tgaaggggga tagtttccgg ctgtcacagg tgctgaacaa
      661 cctgatcagc aatgccatca agtttacgct ggaagggttc gtcaagttga aggtagagat
      721 ggcggagcgg cagggagcca cttatgtcac gcgtttttcg gtgatcgatt cagggatcgg
      781 gataccggag gataaactgg acaagatctt cgagcagttt acgcaggcgg atagcgatac
      841 gacgaggctg tatgggggaa cgggactggg attgtcgata tcgagcaggc tggtggagct
      901 gatgggaagc tcgatcctgg tggcgagctc gcccgggaaa ggatcggatt tccatttcac
      961 gttgccgttg ctgcaggggc atcagaccga cgctgcgact gctacgacca aaatagtgcc
     1021 gaagagcaat gaacaattca agaacaagac gatactgctg gcggaggaca atgtcttcaa
     1081 tgccaatatc gccaggcgtt ttatcaccgg gtggggagct cagctggaga tcgtcgtgga
     1141 cggccggcag gcgctggaat tcgtttcacg ccggaaatac gatctgatat tgatggacgt
     1201 acagatgccc gtgctggacg ggtttgcctg tacgcgcaag atccgcaaac acttcaagga
     1261 cataccgatc atcgccatca ccgcctctcc caagaatgag atcatccatg agatcatgtc
     1321 ctgcgggatg aatgattttg tgagcaaacc gttcaagccc aatgagctta ggaccaagtt
     1381 gctggaatgg ttatagatag ccggggggct ccgggagaga gggtcagccg ccgcgcggcc
     1441 cgagttcgat cacttcacaa tcgcgcatat cttttccatc gatagtgaga cggataatag
     1501 tccgtacggt atgccatccc tgtctgccgg ccgcgccggg gttcatgtga agacagtgaa
     1561 ttttttcgtc gtagatgacc ttcaggatat gagaatggcc gcagatgaag agctgcggag
     1621 tggcagtttg gagctccttt ctgacgccgg ggttatagcg cgggggtgag ccgccgatat
     1681 gggtcatgaa gacgtggacg ccttccgatt gccatcggag tttttcgggg tagacgctcc
     1741 ggatgtcggg gccgtcgata ttcccgtata cacctttcag cggcttgaag tccgacaggc
     1801 gtcgggcgag cccgatcgtt ccaaaatcgc ccgcatgcca gacctcgtca caggtttcga
     1861 aatgccggaa gacgttttcg tcaaggaaat ggtgggtgtc ggcaatcaat ccaatcttca
     1921 gcatggggta aagataatat ttattaattt tggggaacga tcagctatgc ctcactatca
     1981 ttcgctgggg accattcccc acaaacgtca tactcaattt cgccaggggg atggctcgct
     2041 ctactcggag cagttgttct ccacggaagg tttctccaat gattattccc tgctttatca
     2101 tattcatccc ccgacggaga tcattgcgac ggacgctccc tatgatgcac agccgcgcat
     2161 tgccgaagag aagatgctga agcatcgcag cttcgacggg ttcctggtga agccggaagc
     2221 agactttttg caaagccgga aggcggtgct ggtgaacaac gattgtcaca tcgtactggc
     2281 cgcgccggcc gcggggatgg agaattattt ctacaagaat gcggatgccg atgagctgat
     2341 cttcgttcat catggcggtg gaacgttgaa gaccatgtat ggggaactga gctttggata
     2401 cggggattac ctgctggtcc cgagagggac gatataccag atacagttcg atggcccgga
     2461 taaccggctt tttatagtgg aatcatttag ccctatccgt tacccaaaga aatatctgag
     2521 cccttacggg cagctgctgg agcattctcc ctattgcgag cgggatatcc ggggtccgca
     2581 atcattgtcg ccacgcgacg aaaaaggcga tttcctgatc cagacgaaga agaaggggag
     2641 ggtctatggg atccattacg ggcatcatcc ctttgatgtg gtcgggtggg acgggtattg
     2701 ttatccgttt gcgttcagca ttcatgattt cgagccgata acggggcggg tgcatcagcc
     2761 gccgccggtg catcagacct tcgaagggca taatttcgtg gtttgttcgt ttgttccgcg
     2821 gctgttcgac taccatccga tgtcgatacc ggcgccgtat aatcacagca atatcgacag
     2881 cgatgaagtg atctattatg tggacgggga tttcatgagc cgcaagaacg tcacgcgggg
     2941 tatgatcacg ttgcatccgg ggggtatccc gcacggaccg catcccgggg ccgttgcgcg
     3001 cagcatcggc gcgaaagaga cgaaggagct ggccgtcatg gtcgatactt ttcatccact
     3061 gatgttgacg gtggaggcgc tgggcatcga aaatcagggt tacacgatga gctgggcgga
     3121 ataggggccg gggatttcgt atattaggga atgaaacata caagactagc tgcagcggct
     3181 gctatcgtcc ttttctcctg ccgggggaag cagcctgccg atctgatcgt ttatcacgca
     3241 aaaatttata cggtagacag cgcgttcgac actgttcagg caatggcggt gaaggatggg
     3301 cgcatcctgg cgacggggtc ggacgaggac gttcttgcca ggtattccgc gcctgcgatg
     3361 accgatgaag gcgggaaagc cgtgtacccg ggattcatcg atgcgcattc gcatttcata
     3421 tcatatgggg aatcgttgtt catgaccgac ctgttcggtt gtacgagcac ggaagagatg
     3481 gttcagcggg tgaagcaatt cacggatgcc catcctggtc tggcatggat acgggggcgc
     3541 ggatgggatc agaataaatt cccggggaag gcctttcccg acaatacttt gctgaatcag
     3601 ctgtttccaa ataccccggt ggtcctcgga cgggtggacg gtcatgcgtt gctggccaat
     3661 gcgaaggctt tggagctggc gggtgttcat gccgggcagc agatcggagg aggcgtcatc
     3721 gagacgaaga atggccggtt gaccgggatc atgatcgaca atgcgcagcg tttggtttat
     3781 gcgcacgagc ccgatctttc tccggctgag tttttggaac gggtggatgc ggcgcagcag
     3841 aaatgtttcg cgcagggttt gacgaccgtc gcggattgtg gtatcgacag gctggccatc
     3901 gaggcgatcg actcgctgca aaagacgggc aagttgaaaa tgcgtgtcta tgctttgttg
     3961 agcgaccgtc cgtacaatct cgatcactat ttgccgcgcg ggccctataa aacggaccgg
     4021 ttgtatgtcg gtggagtgaa ggcatatgcc gacggtgcgc tgggtagccg gggcgcctgt
     4081 ttgctcgcgc cttacagcga caaaccgggg tgggtgggtt ttctgttgag tcctcctgca
     4141 cattttgatt cactggccgc gcgattagcg ccgacggatt ggcaattgtg cacgcatgcc
     4201 atcggggatt cgggcaacag gatgatcctg caggtgtaca acaaatatct gaaaggaaag
     4261 aatgacaggc gctggcgtgt cgagcatgcg caggtgatcg atgaggcgga tttcgatctg
     4321 ttcggtaagg cttcgatcgt cccgtcggtg caacccacgc atgccaccag cgatatgtat
     4381 tgggcgggtg agcggctggg tccgcagcgt ttgaagaatg cgtatgcttt caaacgactg
     4441 ttgcagcaga atggctgggt ggcgttgggg acggatttcc cggtggagga tatcagcccg
     4501 ttcaaaactt ttctggcggc cgtattcagg gtcgatgcga agggatatcc gtcgggtggt
     4561 tttcagcccg agaatgcgct gagcagggag gagaccatcc gggggatgac gatatgggcg
     4621 gcaagggccg atcgttggga ggacaggatc gggagtctcg aggcaggcaa ggtggccgat
     4681 ttcgtggtgt tggataaaga tatgatgcag gtgccggctg gagaggtgct tagtgtgaag
     4741 cctgtggcga catattcgtc ggggatgcag gtgtttggag cgggcggctc aaaataaagt
     4801 tagcatggcg ggttagcggg gtgggcgcgt attttcgtgg actaaatgaa agcaatatgg
     4861 aatacagaca tctcggtgaa tcgggggtga aggcttctgc gatcactttt ggcgcgtggg
     4921 ccatcggcgg ttggttatgg ggaggcagcg accgaaagga cgccgttgcg gccatccggg
     4981 cttcctatga cgaaggcgtg acgtctatag ataccgcggc ggtgtatggg atgggtttga
     5041 gcgaggagat cgtgggtgag gcattgcagg ggataccccg ggactcagtt cagatcatga
     5101 ccaaattcgg gatgcgatgg gatatgactc gcggggattt tggcttcaag agtcaggata
     5161 atgagggacg gccgctggac atctacaagt atggaggaaa agagagtgtg atcagggaat
     5221 gcgaggatag tttgaggcgt ttgaggacgg actatatcga cctgtaccag cagcactggc
     5281 cggacataac gacgcctatt agcgaaacga tggaagcgct ggatgaattg atgacagcgg
     5341 ggaagataag ggcagcggga gtttgcaact acgatacggc acagatggca gaggcggaca
     5401 agacgatcag gctggcttcg gaccaggtgc cgtacagcat ggtcagacgc gatatcgagg
     5461 acgagctggt gccttattgt ctttcgcata agaaggccat attggcgtat agtccgctac
     5521 agcgaggatt gctaacggga aagatccgtc ccggtcatcg tttcaacgag ggggacaacc
     5581 ggggcgacag caaatatttt cggggtgaga atttaacgcg gatcaatgcc ttcctggacg
     5641 aattgcggcc gatggcggaa gagcggggtg cgagtctgac gcagttggtt attcgctgga
     5701 cgctggagag gccgggcatt accattgcat tggtaggggc acgggatgcc cggcaggcgg
     5761 tcgagaatgc cagggcgatg caggtcaggt tgtcggagga tgaaatggcg gttatcgatg
     5821 agaagctggc ggagttgcag ggggcgctgg tgatgtagcg gacggtggtg gcaggaattg
     5881 gggtcggcgg tgcgggagcg gagtacggct agcaggaatt ggggcgtcgg cggtgcggga
     5941 gcggatatgc ttgcgattta gggggaaatc ccgtaaattc ggaaataaca aataaaacga
     6001 tgtcatatgg tcccttttca tgaattgaga atcggcgata ttgtgttgat cgagttcaac
     6061 ggtcagcgta cggagggaga ggttatcgat aaggctgatg cggacagatt ggtttgcgtt
     6121 gacaatgaag cgcaggcttt ttggtacggg tcggaggaat tgtatcctat tcccctggat
     6181 gagaaccagt tattgaaatt cggttttgag aagcaggttc agcccgatgg gtcggtcaag
     6241 tatttgcggg gacctttccg gatcttgttg catgtgccgg gtagttttgc ccatttcgag
     6301 atgtggtaca gggaggacag gcgccatatc atcgagcata tcagtgtcca tcagctgcag
     6361 aatcattatc atcagatgac aaaggtcgat ttggtgaggg gggtcggggc gcaccattag
     6421 gtgggttgaa tgacttgcag gcgggttgaa cgccttggta ttacactttt atttaatatc
     6481 attgccggaa atcagagcgg accgcccgaa aagggtgcga ccggggcctt gccagtggca
     6541 gggcggaggc cttcgccggc cgaaggccga ttaaaaattg acattccggc ttgatttcta
     6601 gctatttttt gccgcaaatg taaattattc ctatctttgc gctcccaaaa acgtatggcg
     6661 aaacaagcac tcattaaaca agatggtatc attctggaag ccttgtccaa tgcaatgttt
     6721 aaggtaaagt tggaaaacgg ccatgaaata ctggcaacca tttcggggaa aatgaggatg
     6781 aattacatac gtatactgcc gggggataag gtaggggtgg agatgagtcc gtatgacttg
     6841 acgagaggaa gaattatatt caggtataaa taaaaatgta acatgacacg aggcgaaagc
     6901 cgactgcatg attcaaataa aaaagacaaa taattcatca tgaaagttcg tgcatccatc
     6961 aaaaagcgca gcgccgattg taagatcgtg aggaggaagg ggaagctgta tgtgatcaac
     7021 aagaagaatc ctcgtttcaa gcaacgtcag ggttaattag ataaaattaa acaagtaata
     7081 gaatatggct cgtatcgccg gtgtagactt accaaaaaat aaaagagggg agatcggact
     7141 gacctatatc ttcgggatcg gtcgttcgac tgcccagtat atccttacaa aggctggtat
     7201 cgaattcgac aagaaggtac accagtggga tgacaatgag ctgaacgcca tccgtaatat
     7261 cattacgaat gagttcaagg tagagggcgc gcttcgttcg gaagtgcaaa tgaatatcaa
     7321 gcgtctgctc gatatcgcct gctatcgtgg tttgcgtcat cgtaagggtc tgcccgtgcg
     7381 tggccagcgt acccgtacca acagccgtac ccgtaaagga aagcgtaaga cggttgccgg
     7441 taagaagaag gtcgccaaga aataattgaa aatgcaccgg cggacgggag tccgctgata
     7501 atcggatcgg ttgcggatgc gggtgcacgc ggaggatcgg tccggtcccg gttggcggga
     7561 tg
//
LOCUS       DASYSV010000598         4891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1148327, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000598 DASYSV010000000
VERSION     DASYSV010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148327"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VGQ51_18630"
     CDS             <1..536
                     /locus_tag="VGQ51_18630"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEV9038663.1"
                     /translation="VGIFNISQKFADLLIFPFFLMHTVLPQLFARHSQEEVGYTRSLF
                     RESNRLMALLAVPLLGVDLLAGPFLLRLFGPAFAAGYTALVYISLAQFLFSLFGPTNT
                     ILMMQGKERSSAICLLVYVGLLVLVSRLLIPVSGITGGALAILISSAGYNILLAVIVY
                     RQYGICSPFLSWIVGRR"
     gene            complement(501..2438)
                     /locus_tag="VGQ51_18635"
     CDS             complement(501..2438)
                     /locus_tag="VGQ51_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038664.1"
                     /translation="MQYSRDKNLLLTVFFLFWLPVVLFFALDHRLLSQVRPALFWFNR
                     DLAELTLIGIGLPRWMIDHPASFAAMDGLAVLLPIILLRSAIRNKRFSSALGWIFTVF
                     LVLYILLADIFWQVHLEPFVIYWLLSLAFLTNSERRFYGILRICRYYFLYIFVSAAIW
                     KIARGAVFNGQEMSDILLRHQGYLLAGDCDTWTCRFCSWLIDHPGPAQTLYIAAAVLE
                     AFFLIGFFTRRYDRLLIALAVLFVVADLVVMRIPYWTLLVGTVTLWIGSDVRRPAIVI
                     YETTHHENLPALLDLCETRFPRVIVFLKESSWQNLSGGQSPSDRWPRTEFIRQPAGSR
                     NRPFIRSLFSFIRRHHCSHLHLSTLDNNLLVFALRIAMAGNVHISLTVHEVNAWFRDS
                     FRSVRKLSESLAKRFLRRRVRHYTVFLPAIADHFRQRMPDAVTVFIPSRFYMGPSPRG
                     AALPLTIVVPGSVEANRRDYESVAAFFETWRPARPVRLILLGDSDSPYGSAIVARFAR
                     LESSEFLFTSYKGYIPERLYEQKLREADLIWSPLRVHKTGGHDEPETYGLTTASGLTA
                     DLLLNNIPALVPAEFGLPEPFRKALLPYSSMEDIARLIDIGRQAGLRGDIDKAFVFFR
                     KENFFDAFDRLTSPDYPGQKG"
     gene            complement(2448..2930)
                     /locus_tag="VGQ51_18640"
     CDS             complement(2448..2930)
                     /locus_tag="VGQ51_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038665.1"
                     /translation="MKGLFICFIVLQIGLDLAHSVTFFPFTHYGMFSAVEPRPDSVLV
                     YKIVADGRRLQPADFTIYRWDMVQTPLEAYDKQTATHDYAFDKDRLQQGMRWAGAGSL
                     YSALKTNLDNTGDFPSWYKSYLGGLLGHRIGSLRVEKAWYRWQDGRLLPIGKESWINL
                     "
     gene            2961..4748
                     /gene="lepA"
                     /locus_tag="VGQ51_18645"
     CDS             2961..4748
                     /gene="lepA"
                     /locus_tag="VGQ51_18645"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEV9038666.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLEMTGTISERDMQAQVLDDM
                     DLEREKGITIKSHAIQINYTHKGEKYILNLIDTPGHVDFSYEVSRALAACEGALLLVD
                     ATQGIQAQTISNLYLAIENDLEIIPVINKIDMDGAMVEEVKDQIIELIGCKPEEILLA
                     SARTGVGVAEILDSIVARIPPPAGDPDAPLQALIFDSVFNSFRGIIVYYRILNGSIKK
                     GDNVQLISTGLTYGADEVGVLKLTLDPRAEVGTGDVGYIITGIKNAKEVKVGDTITLA
                     NNPHPDMIHGFEEVKPMVFAGIFPVNTDDFEELRDCMDKLQLNDASLTYELETSQALG
                     FGFRCGFLGMLHMEIIQERLEREFNQTVITTVPNVSFIATTTKSEVIIVNNPAEMPDP
                     VRIETIQEPFIRAQIITKPEYIGNIMTLCLGKRGILINQSYLTTTRVELIFEMPLTEI
                     VFDFYDKLKSQTRGYASFDYHPLDKRESDIVKMDILLNGDKVDALSALIHRSRSTDFG
                     RKLCEKLKELLPRQQFQIAIQAAIGAKVIARETISALRKDVTAKCYGGDISRKRKLLE
                     KQKEGKKRMRQIGNVEVPQEAFLAVLKLD"
BASE COUNT         1210 a         1261 c         1411 g         1009 t
ORIGIN      
        1 acgtcgggat cttcaatatc tcccaaaaat tcgccgatct gctcatcttc ccttttttcc
       61 tgatgcatac cgttttgccg cagctgttcg cccgtcattc gcaagaggaa gtgggttata
      121 ccaggagtct gttcagggaa agcaaccggc tgatggccct gctggcggtg ccgctgctcg
      181 gggtggatct gctggctggt cccttcttat tgcggctgtt cggtccggct ttcgccgcgg
      241 gatatacggc gctggtatat atcagtctgg cacaattcct tttctcgctg ttcggcccga
      301 ccaataccat tctcatgatg cagggaaaag agcgttcgtc cgccatctgc ctgctggtct
      361 atgtgggact gctggtgctc gtcagccggt tattgatacc cgttagcggc attaccggcg
      421 gcgcgcttgc catcctgatc agcagcgcgg gatacaatat attgctggct gtcatcgtct
      481 accggcagta tggcatctgc tcaccctttt tgtcctggat agtcgggaga cgttagccgg
      541 tcgaaagcat cgaagaaatt ttcttttctg aagaagacaa aggccttgtc gatgtcgccg
      601 cgaagacccg cctggcggcc gatgtcgatg aggcgcgcga tatcttccat ggaagaatag
      661 ggtagtaatg ccttgcgaaa aggttccggg agaccgaatt cagccggcac cagtgcaggg
      721 atattgttca gcagcagatc ggcggtaaga ccgctggcgg tggtcagacc ataggtctcc
      781 ggttcgtcgt ggccaccggt cttatgcacc cgcaacgggc tccagatgag gtcggcctcg
      841 cgcaacttct gctcatatag tctttcgggg atatagcctt tgtagctggt gaacaggaat
      901 tcggatgact ccagccgcgc aaaccgggcg acgatggcgg agccataggg ggaatcgctg
      961 tcgcccagca ggatcaggcg taccgggcgg gcagggcgcc aggtctcgaa aaatgcagca
     1021 accgactcat aatccctccg gttagcctcg acagaaccgg ggacgacgat ggtcaaaggc
     1081 aaggcagcgc cgcgaggcga cggtcccata taaaaacgcg aagggatgaa tacagtcacg
     1141 gcatcgggca tccgctggcg gaaatgatcc gctattgccg gaaggaacac ggtatagtgc
     1201 cgtaccctgc gtcgaagaaa tcgtttggcc agcgattcgg agagtttgcg caccgaccgg
     1261 aaggaatccc ggaaccaggc attcacttca tgcacggtca gactgatgtg cacatttccg
     1321 gccatggcta tccgaagagc gaagacaagt aaattattgt cgagcgtgct cagatggaga
     1381 tggctgcagt ggtggcggcg aatgaaggaa aacaggctgc ggataaaagg ccggttgcgc
     1441 gatcccgcgg gttgccggat gaactccgtt cggggccagc gatcggaggg gctctgcccg
     1501 ccggagaggt tttgccagga agattccttc agaaaaacga tcacgcgcgg gaaacgggtc
     1561 tcgcagagat cgagcagggc gggcaggttc tcatgatgag tagtttcata gatgacgata
     1621 gccggccggc ggacatcgga cccgatccaa agagtgaccg tcccgaccaa tagtgtccag
     1681 tagggaatgc gcatgacaac caggtcggcc acgacgaaga ggacggccag cgctatcagc
     1741 aaacgatcat atcttcttgt gaaaaaaccg atgaggaaaa aggcttccag gaccgcagcc
     1801 gcgatataca gcgtttgtgc tggcccggga tgatcgatca gccagctgca gaaccggcag
     1861 gtccatgtat cgcagtcccc ggccaacaga tagccctgat gtcgcaatag gatatcgctc
     1921 atttcctgtc cgttgaaaac cgcgccgcgt gcaattttcc atattgcggc agagacgaag
     1981 atgtagagga aataataccg gcatatccgg aggatgccgt agaaacgccg ctcgctgttg
     2041 gtcagaaagg ccagcgagag cagccagtag atgacaaacg gctcgagatg cacctgccag
     2101 aagatatcgg ccagcaggat ataaaggacg aggaaaacgg taaagatcca gcccagcgca
     2161 gatgaaaaac gcttgtttcg gatggcgctt cgcaggagga ttatcgggag aaggaccgcc
     2221 agcccgtcca tggcggcaaa actggccggg tggtcgatca tccagcgtgg cagaccgatg
     2281 ccgatcaggg tcagttcggc cagatcgcga ttaaaccaga agagtgccgg tcgtacctgc
     2341 gacaaaagcc ggtgatcgag ggcgaaaaaa agcacgaccg gcagccaaaa caggaaaaag
     2401 acggtcagta agaggttttt atcgcgggaa tactgcatga tcgccggcta tagatttatc
     2461 caggattctt ttccgatcgg gaggagcctg ccatcctgcc agcgatacca ggccttttca
     2521 acacggagac ttccgatccg atgacccagc aggccgccga gataggattt ataccaggac
     2581 gggaaatcac cggtgttatc taaattggtt ttcaaggcgc tatacagtga acccgcacct
     2641 gcccaccgca tgccctgctg cagccggtcc ttgtcgaagg cgtaatcgtg tgtcgcggtt
     2701 tgtttgtcat aggcctccag cggcgtctgg accatatccc atcggtagat ggtgaaatcg
     2761 gcaggttgca aacgccttcc atccgcgacg attttgtaga ccagaacgct gtccgggcgg
     2821 ggctcgacgg cggaaaacat accgtaatgc gtaaaaggga aaaatgtcac cgaatgagcc
     2881 agatcaagcc cgatttgcag gacgataaag cagatgaaca accccttcat agtgtgtgaa
     2941 aatacgtaga tttgcagcga atgaaaaata tacggaattt ttgcattatc gcccatatcg
     3001 accacggaaa aagcaccctg gcggaccgtt tgctcgaaat gacgggcacg atctccgaaa
     3061 gggacatgca ggcccaggta ttggacgata tggacctcga acgggagaaa ggcattacga
     3121 tcaagagcca tgcgatccag atcaactata cccataaagg ggagaaatat attctgaacc
     3181 ttatcgacac tccgggacac gtagatttca gctacgaggt aagtcgggcg ctggccgctt
     3241 gtgagggggc gttgttactg gtcgatgcca cacagggcat tcaggctcaa accatttcca
     3301 atctttatct cgccatcgag aacgatctgg agatcatccc ggtgatcaat aagatcgaca
     3361 tggacggagc aatggtcgaa gaggtgaagg accagatcat cgagctcatc ggctgcaaac
     3421 cggaagagat ccttctggcg agcgcgcgta cgggagtcgg tgtggccgag atccttgact
     3481 ccattgtggc gcgtataccc cctcccgcgg gtgatccgga cgcgcctttg caggcgctga
     3541 tcttcgacag cgtgttcaat tcctttcgcg gaatcatcgt atactaccgt atcctgaacg
     3601 gcagtatcaa aaaaggcgat aacgttcaat tgatctcgac gggtctcact tatggggccg
     3661 atgaggttgg cgtcctgaag ctaacactgg acccacgggc ggaggtggga acgggcgatg
     3721 tcggatacat catcaccggc atcaaaaatg ccaaagaagt gaaagtgggg gatacgatca
     3781 cgttggccaa caatcctcat ccggatatga tccacgggtt cgaggaggtc aagcccatgg
     3841 tattcgccgg catcttcccg gtaaatacag atgatttcga agagctgcgc gattgcatgg
     3901 acaagctcca gctgaacgac gcctcgctga cctatgagct ggagacctcg caggcgcttg
     3961 gtttcggttt ccgttgcggt ttcctgggaa tgcttcacat ggagatcatc caggaaaggc
     4021 tcgaacgcga attcaatcag acggtgatca ccaccgttcc caacgtcagc tttatagcca
     4081 ccacgaccaa atcggaggtc atcatcgtca ataacccggc tgaaatgccc gatccggtac
     4141 ggatcgagac catccaggaa ccctttatcc gggctcagat catcaccaaa cccgagtata
     4201 tcgggaatat catgacgctg tgtctgggga aaagaggcat tcttatcaac cagtcctatc
     4261 tgaccactac acgggtagag ctgatcttcg aaatgcccct gaccgagatc gtttttgatt
     4321 tttatgataa gctgaagagt cagacgcgcg gatatgcttc cttcgattat catcccctcg
     4381 ataaacgcga gagcgatatc gttaaaatgg atattttgtt gaatggggat aaggtagatg
     4441 cgctgagcgc gctgatccac cggagcagga gcacggattt cgggcgcaaa ctttgcgaga
     4501 agctcaagga actgttgccg cgtcagcaat tccagatcgc catccaggcg gctatcggcg
     4561 ccaaggtcat tgcccgggaa acgatcagcg ccctccggaa agacgttacc gccaaatgtt
     4621 atggcggcga tatcagccgg aagcgcaagc tgctggagaa acagaaggag gggaagaagc
     4681 ggatgcggca gatcgggaat gtggaagtgc cccaggaagc cttcttagca gtcctgaaac
     4741 tcgactaaaa gaaatcaaag actatgcgga ccccgcttca gaaaggagaa aagatcctgc
     4801 tcgtcaccta tagcagctgg acatcgctgg tcgtgccggc gctttttgcg ctgatattca
     4861 cggtcggcgg tctgatcgtc gtttttcatt t
//
LOCUS       DASYSV010000599        13871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1148875, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000599 DASYSV010000000
VERSION     DASYSV010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13871
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148875"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VGQ51_18650"
     CDS             complement(<1..947)
                     /locus_tag="VGQ51_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038667.1"
                     /translation="MLKNYLKVALRNLWKSKGFTAINIIGLASGIGVCLLIVLYVTDE
                     LSYDRFNTNASRIYRLDADIYFNNTLFDAVVSPKPLASTLKKDYPQVEEMVRIGSVRD
                     VLVKKGNNWIQDHHYAYADSTLFRVFTIPMISGNPQTALNDPHSIVIDESAARRYFNS
                     TDVLGKTLELEDHNLCKITGVIRDMPRQSHFHLSFIQPISHSWQGDNIDWLSNNWYSY
                     ILVRPGITRDVMQSRVDAVTNNYLGKQLQDLLHTSLGELAHSGNYFKYKLMPLTDIHL
                     HSNKSYEMEANGNIAYVYVFSFIAILILVIACVNFMNLST"
     gene            complement(1033..1464)
                     /locus_tag="VGQ51_18655"
     CDS             complement(1033..1464)
                     /locus_tag="VGQ51_18655"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEV9038668.1"
                     /translation="MHNRFSTEVKETISFSAEEAHRMGSPVVSAGHLLLGLVRQRHNR
                     AVTLLTDDLHIPLSALVVAIEETLPPGVEGGKAGSWRLPLDRGAERAIRASVAEAKRS
                     GSRTVDAEHLLSSLFNDRDTHLFAILGRWGIQRLPPGAGGQ"
     gene            complement(1468..1935)
                     /locus_tag="VGQ51_18660"
     CDS             complement(1468..1935)
                     /locus_tag="VGQ51_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038669.1"
                     /translation="MTDEKLGNYLKEISKPEAPPARGQEMLKVTLMNARRSSVIGAVL
                     IILPGGLILLFLLQNSLHLAPGVTRWLAGEGTFLPLPARAIVAFVFLVGFPVLAVILN
                     MLAITWYRYDRARKELTVTVRMRWINVLIIIAGSALASFYVLHLLADTIMNKG"
     gene            complement(1961..2536)
                     /locus_tag="VGQ51_18665"
     CDS             complement(1961..2536)
                     /locus_tag="VGQ51_18665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV9038670.1"
                     /translation="MNEKQLIEWARVNPRAFGELYDVYYPKIFGYIYRITGDHALAGD
                     IASETFLKAWIAIGSFKWKGISISSWFFRIATNQLNQYFRRQRYTPRTMTDLGVFDMA
                     AWDNRHAIAEDDITQKIDRFEEFEAVRRLLKRLPSDYQQVIALRYFEELSIRDIAQIL
                     KKKEGTVKSLLSRGLDKLKKMMDESATKGRP"
     gene            complement(2533..3315)
                     /locus_tag="VGQ51_18670"
     CDS             complement(2533..3315)
                     /locus_tag="VGQ51_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038671.1"
                     /translation="MKHIVFAILLAAAVATAPSMWAQNQSVRLTETQWKAVEGIYRNW
                     QNSDMNLRFEGHGDTLFAQPLWLRNDHYQPLVRKEMAHTPEQLRPFEGLYQMEGRNSR
                     LARLSERNNRLILQEIGNGGELSFVIQSDSSFYLTGNPVGTLDFSKDDSGHIVEMVFF
                     KQQFWVKIKKVALTTATIRQYEGKFQSRDDPDNQLRLIAQDSNLVVKQVWDGKEIVLS
                     PLTETFFYNEAQSYPLAIVLGADGKVKSIMILSSQEFDKVPE"
     gene            complement(3323..4225)
                     /locus_tag="VGQ51_18675"
     CDS             complement(3323..4225)
                     /locus_tag="VGQ51_18675"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV9038672.1"
                     /translation="MKFLPGLLIFLYLAPLFLYAGAPPNSRQYVSDYENVIGTSMELK
                     VSTTSPAAAEKAQKAVMHEIGRLSAILSAYNRNSEFSRWERTAHVPVRVSPELFEVLG
                     LFDQWRIRTAGALDASVEAVTRVWKEAAGQGRLPGAGQLAQAVAEVKQRHWELDSITH
                     TATHLSNTPLMLNSFAKNYIIRRAADAGRAAAGADAIVVNIGGDLVVTGNVPETVAVS
                     DPKADAENDAPVTLLRISNAAVATSGNYRRGELIDGHWNSHIVDPRTGIPADDPDNQL
                     QLIARGGNLVIVILSSQEFDKILE"
     gene            4376..5197
                     /locus_tag="VGQ51_18680"
     CDS             4376..5197
                     /locus_tag="VGQ51_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038673.1"
                     /translation="MTKRCLASVFFIALLAPGRGIHAQDNYEIQVYASPTVGKDTTMV
                     ELHSNYSFIGAPGADGVVSTNHMLRETIEITHGFTPWFEMGFYIFNCIGDMGRTNYVG
                     SHLRPRVMAPVSWNWPVGVSVSFEGGFVKDGYDPDTWTLEIRPIIDKQLGPVYLALNP
                     VVDLTFAGADKGRGVGFAPDFKFSYQIGKVWAPGIEYYGSLGPFRKFDPIDQQQHQLF
                     IAVDADFDPRWEFNAGYGFGWGIDKSIFKVILGRRFGGGKHAHTLGGTPHTVFRF"
     gene            complement(5194..5796)
                     /locus_tag="VGQ51_18685"
     CDS             complement(5194..5796)
                     /locus_tag="VGQ51_18685"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV9038674.1"
                     /translation="MENGAASAGDLRVIVPWLFLQTPVLTSSNIRIFFSFLKIGAILY
                     GSGYVLFAFLDSELVAKGLLPRQQLVDAIAVGQFTPGPVFSSVTFIGWQMGGLRGAAL
                     ATLAVFLPSFVFVAALNPLLPLMRRSAFFSTFLDGVNVASMALIGVVCIRFGHDTVTD
                     WRTILIAIAGFVATFGWRKLNSAWVIFGGAMLGWVLRMAS"
     gene            complement(5763..6344)
                     /locus_tag="VGQ51_18690"
     CDS             complement(5763..6344)
                     /locus_tag="VGQ51_18690"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV9038675.1"
                     /translation="MENATSQNGPAPATPPGDLVRLFIRLGLTAFGGPAAHIALMQKE
                     VVDKRHWMDHQHFLDLLGATNLIPGPNSTEMAIHIGRERGGGKGLLIAGACFILPAVV
                     ITGVIAWLYQRYGRLPQVADLIYGIKPAVIAVIAGAVYPLARRALKNIQLWLLLVAAF
                     AAGLWWSEGEIAILFGAGVVYSMWRMALRPRGI"
     gene            complement(6360..6854)
                     /locus_tag="VGQ51_18695"
     CDS             complement(6360..6854)
                     /locus_tag="VGQ51_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038676.1"
                     /translation="MKKISISIGGVFVLFIACSQPHDNTAVLQQQIDTLQQQLRNVYT
                     PGLGEFMSGIQVHHEKLWFAGKTENWKLADFEIGEMQEAIDDIKKYCTDRPEVASLPM
                     LQPALDSVSHAVSRADGDAFRNGFVYLTATCNNCHRATKHEFNVIQLPTTVPFSNQVF
                     EKPR"
     gene            complement(6931..7176)
                     /locus_tag="VGQ51_18700"
     CDS             complement(6931..7176)
                     /locus_tag="VGQ51_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038677.1"
                     /translation="MAKEIIPIEFNGQGIEYKGWATPSEQLHDDGFPQSYHVVLNKVY
                     FGNLSLDRGKWIADEPRPRELVVAVGACLDNLHVGVQ"
     gene            complement(7224..8879)
                     /locus_tag="VGQ51_18705"
     CDS             complement(7224..8879)
                     /locus_tag="VGQ51_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HEV9038678.1"
                     /translation="MQKGLLLFCCLLIASPHPRDPEVAPTIGFFMDDWKPKTFVVPAF
                     REGAVAGRASATLRIDPDSVITRIPPAEFGHNADTWMGTMVTQPVLMDHLKNLQSHII
                     RWPAGSGSDGYFWNSGPGSMPSDLPVEVLDTRGKRTAPHWFYGRPRGRFGASVDDYYD
                     LLAATGNEGLITVNYGYARYGTGPNPVATAAHLAADWVRYDHGRTRYWEIGNENYGNW
                     ELGYRIDTATNRDGQPQLLTGRLYGEHFRIFADSMRNAAKEVGSTIYIGAVMHETPPK
                     LFDPPLTRDWNSGMIPAAGDMPDFYAVHNYFTPWNANTGAADILHDAVQVPGDMMDYV
                     LKCLRENGAATKPIAMDEWNMFASGSKQQVSNISGLFSIIVMGEALRNKYGLAARWDM
                     LNGWAGGNDHGLFSSGDEPGVRRYSPRPSFYYLYYLQRMLGDRLVVSHLAGDTSLRAY
                     ASSFSSGEIGAALVNTSTHASTVEIEIPHFSRGRYYWYSLEGSDDNGEFSRKVAVNGD
                     STMAAAGGPADYAALRAFGASAKGGVRVMVPARGAVFLVVRRR"
     gene            complement(8891..12235)
                     /locus_tag="VGQ51_18710"
     CDS             complement(8891..12235)
                     /locus_tag="VGQ51_18710"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEV9038679.1"
                     /translation="MIRITVISIFFIGALSCNPVHDRRGLHFTLLPASSTGIGFANNI
                     TENDTLNMFVNEYTYMGGGVGVGDFNRDGLPDLFFAGNQVSSRLYLNKGGMHFEDITQ
                     KAGVCTGVWCTGVSVVDINNDGWPDIYVCVSGQAPGARRRNLLFINQHDLTFKEEAAA
                     YGLADTGYSTQAVFFDYDRDGRLDMYLVRHTLADRLPNNIRDPSVDTANIAADKLFHN
                     EGKAPGTDHPVFRDVSRQAGIVEDGNGLGVIAGDFNGDGYPDLYVDNDYIRNDLLWLN
                     NGNGTFTNCIATAVRHQSYSSMGVDAADINNDGLPDIVTLDMQPENNFRKKMMYSFLS
                     EQRHQLEMEKGYEPQFIHNMLQLNNGTRWVDGRTEPFFSEIGEMAGIAETDWSWSVLI
                     ADFDNDGWKDMHITNGLGRDPTNIDFLEYAHNTVLATGIPENEIGQRRKFMERLSAMG
                     PLSLRNYVYRNKDGISFEDISDSAGIHEKGIANGAIYADLDNDGELDIVTNNINAPAT
                     VRHNDSRPAHYLTLRLSGDSLNRDGIGATVTAYGNGEVQTVTQYPVRGFLSTVDSRLH
                     IGCGAAAPDSLKVVWPDGRMQVLGRPGVDTILTLDWKSAVVDPPAVQDADPLFEDATR
                     RLKTDFRHKETFFYDFKFQPMLLQKFSQEGPFISTGDVNGDGLEDFFIGGAFKQSGRL
                     YLQNRDGSFTGRDLVTGNKYEEDMQSVFFDADGDKDPDLLVVGGSSEFEDHSSFYCPR
                     LYLNDGKGNFRLDSVAFSPLVRTPAKAVAVADMDGDGDMDIFIGGRVSLGSYPRPPDS
                     YILRNDHGRFTDVTDAVCPALRHIGMINAAVWADIDGDGKPDLIVAGDWMPIRIFTND
                     GHTLTEITGRSGLKDLSGFWRSLQIVDVDHDGHPDIVAGNLGTNNPWHISSDRPAELI
                     AKDFDGNGIMDPVFCYYIRGDDGTYRLSAGVSRDNWAMEMPSIKKKFDDNAKYASAPM
                     EEIFTPEMMQGALVLDCKEVRSGWFRNDGKGNFRFHPFPAMAQIAPVNATAAGDVNGD
                     GFTDLILAGNDYEASLMSGRYDASYGLLLEGDGRGGWRVMPPASDGLVLDGDVRDIKL
                     IHAGGDRYLLTAVNDQPMRAYRLSKR"
     gene            complement(12236..>13871)
                     /locus_tag="VGQ51_18715"
     CDS             complement(12236..>13871)
                     /locus_tag="VGQ51_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HEV9038680.1"
                     /translation="SSYNPSDPSLAPTIGFFGNAWAQRTFNAPSDNMVSAATTAVSDS
                     LTIDVNKVQVKTPPYIFGNNSNLWMGQIVTEANLVQYIKDLSPNIIRAPAGSVSDTYF
                     WNGTTANPHPADAPDSIITSGNLGSIGSWYGGDAASWTLSLSNYYSLLAQTNSTGIIT
                     INYGYARYGTGPNPVAAAAHLAADWVRYDNGKTKFWEIGNEIYGSWEAGFQIDVSKNK
                     DGQPATVTGALYGKHFNVFADSMRAAAQSIGATIYIGAIVYGSPPQSYDNNTVQTWNQ
                     GVLANAGTTADFFIDHDYYTAYAANSTVPQILGTGTTEAPTAMTYIKQQLQGAGVANK
                     PIALTEWNIQAVGSKQAVSYIAGMHAALTLGSFLKSGLGEASRWDLANGSTPWGDDMG
                     MFNNGDEPGAPMWNPRPAFYYMYFFHRMFGDRMVYDTLKHVQGNNDLTTYSSTFSSGQ
                     AGTVIINTGNFNHVLSIDFQHFPAGKKYHWFVLTGGTDNAPFSGQVYVNGAGPSTSTG
                     GPLNYGSISANAATLNGTIKIAVPPMSVIYLVADGK"
BASE COUNT         3082 a         3956 c         3707 g         3126 t
ORIGIN      
        1 gtgctgagat tcatgaaatt gacgcaggcg atcaccaata tgagtatggc gatgaacgag
       61 aacacatata cataggcgat attcccattg gcctccatct cgtaggattt gttggaatgc
      121 agatggatgt cggtcagggg catgagcttg tatttgaaat aattccccga gtgcgccaat
      181 tcccctaacg aggtatggag aagatcctgc agctgtttgc cgagatagtt attggtgacc
      241 gcatccacac ggctctgcat gacgtccctg gtgatgcccg gtcttacgag gatatagctg
      301 taccagttgt tgctcagcca gtcgatgtta tcgccttgcc aggagtggct gatcggttgg
      361 atgaagctga gatgaaaatg cgactgacgc ggcatgtccc tgatcacacc cgtgatcttg
      421 caaagattat ggtcttccag ctccaatgtc ttaccaagca catcggtgct gttgaaataa
      481 cgccgggcgg cgctttcgtc gatgacgatg gaatggggat cgttgagcgc ggtctgggga
      541 ttgcccgata tcatagggat cgtgaatacc cggaacaggg tggagtcggc atacgcataa
      601 tgatggtcct gtatccagtt gttccctttt ttgaccagca catccctgac gctgccgatc
      661 cttaccatct cttcgacctg tggatagtct tttttgagcg tcgaggccag gggcttaggg
      721 gataccaccg catcgaacag ggtgttgttg aaatagatgt cggcatcgag acggtaaatt
      781 cggctggcat tggtgttgaa ccggtcatag ctgagctcgt cggtcacgta gaggacgata
      841 agtagacaaa cgccgatacc ggaggcgaga ccgatgatat tgatagcggt aaagcctttg
      901 cttttccata ggttgcgaag agcgaccttg aggtagtttt ttagcatgcc ggaacgtttt
      961 tccaaacctt cgacgagcga aggcgggcga aagttacgct aacggatgcc ctttttgaca
     1021 aaaatccata agttattgcc cgcccgcccc cggcggcaac cgctgaatac cccatctgcc
     1081 caatatcgca aacaagtggg tatcccggtc attgaacaag gaagacagca gatgttccgc
     1141 atccaccgtc cggctacccg accttttagc ttccgccacg ctcgcccgga tggccctttc
     1201 cgccccccgg tccaacggta gccgccagga cccggctttt ccgccctcca ctccgggtgg
     1261 taaggtttct tctatcgcca cgaccagtgc ggacagcggt atgtgaagat cgtccgtcaa
     1321 tagggtaact gcccggttat gtctctgccg gacaagcccc agcaacagat gcccggcgct
     1381 gacaaccggg ctgcccatcc ggtgggcttc ttccgcgctg aatgagatgg tttctttgac
     1441 ttcggtagaa aaacggttat gcatagatta tcctttgttc attatagtat ctgccagcaa
     1501 atgcaggaca tagaaagacg ccagcgcgct acccgcgatg atgatcagaa cgttgatcca
     1561 ccgcatgcgt accgtgacgg tcagctcttt ccgtgcccga tcgtacctgt accaggtgat
     1621 tgccagcata ttcaggatga ctgccagcac cggaaaaccc accaggaaaa caaacgcgac
     1681 gatcgcccgt gcgggcagcg gcaaaaatgt tccctcccct gccagccatc gggtcacacc
     1741 gggcgccaga tgcagactat tctgtaaaag gaacaataag atcagccctc cagggaggat
     1801 gatcaatacc gcgccgatga cggaagaccg ccgggcattc atcagcgtca ccttcagcat
     1861 ttcctgtccc ctggcgggcg gcgcctcggg tttggatatc tcttttaaat aattgcccaa
     1921 tttttcatct gtcataagag acgatttaat gatataatgt ctagggacgg cccttcgttg
     1981 cggattcgtc catcattttt ttcaatttgt cgaggccccg ggacaacagc gatttgaccg
     2041 tcccctcctt tttcttcagg atctgcgcga tgtcccggat agacagttct tcgaaatacc
     2101 tgagtgcgat cacctgctga tagtccgacg gcaatcgctt caacagccgg cggacggcct
     2161 cgaactcttc gaacctgtcg atcttttggg taatgtcgtc ctccgcgatc gcatggcggt
     2221 tgtcccaggc ggccatatcg aatacgccca gatcggtcat cgtgcgcggg gtatagcgtt
     2281 ggcgtcggaa atactggttg agctgattgg tggcgatgcg aaagaaccag gatgagatgg
     2341 agatcccctt ccatttaaaa gagccgatcg cgatccaggc ctttagaaac gtctccgaag
     2401 cgatatcgcc cgccagcgca tgatcacccg tgatccggta gatatagccg aatattttgg
     2461 gatagtagac gtcgtataat tcaccgaagg ccctggggtt gacgcgcgcc cattctataa
     2521 gttgcttttc gttcattccg gcactttgtc gaattcctgc gacgaaagga tcatgatcga
     2581 tttgaccttt ccgtccgcgc cgagcacgat cgccaggggg tacgattggg cttcgttata
     2641 gaaaaacgtt tcagtcaggg gagagagaac gatctccttt ccatcccaaa cctgtttgac
     2701 cacgagatta ctgtcctgcg cgatcaatcg cagttgattg tcgggatcgt ccctggattg
     2761 gaatttccct tcgtattgcc ggatcgtcgc tgtggtcagc gcgacctttt tgatcttcac
     2821 ccaaaattgt tgtttgaaga acaccatttc gacgatatgt ccggaatcat ccttcgaaaa
     2881 gtccaacgta ccgactggat tgcccgtaag gtaaaagctc gaatcggact gtatgacgaa
     2941 cgagagttcg ccgccattcc caatctcctg caggataagc cggttgttgc gttcggaaag
     3001 cctggctaac cggctattcc gtccctccat ctggtatagc ccctcgaagg gccgaagctg
     3061 ttcgggcgta tgggccatct cctttcgtac cagcggctga taatgatcgt tgcgaagcca
     3121 aagcggctgg gcaaataacg tatcgccatg tccttcaaat cgtaaattca tatcggaatt
     3181 ctgccaattg cgatagatac cttctaccgc cttccactgg gtttcggtga ggcggacgga
     3241 ctgattttga gcccacattg atggcgcagt ggctacagcg gcagccagca atatcgcaaa
     3301 gacgatatgt ttcatttgag gatcattcca gtattttatc gaattcctgc gacgacagga
     3361 tcactatgac gagatttccg ccccgcgcga tcaactgcaa ctggttgtcc ggatcgtcgg
     3421 ccggtattcc ggtccttggg tcgacgatat gcgagttcca gtgaccgtca atcagctcac
     3481 cgcgacgata attaccactg gtggctacgg cggcattgct gatacgaagc aacgtgaccg
     3541 gcgcatcatt ttccgcatcc gccttcggat cgctgaccgc aacggtctcc gggacattcc
     3601 ccgttacgac gaggtctccg ccgatgttga cgacgatcgc atccgctcca gcggcggccc
     3661 ttccggcatc ggctgcgcgc cggatgatat aattcttcgc aaaagagttg agcatcaggg
     3721 gggtattact aagatgggta gccgtatgcg tgatactgtc caattcccaa tgccgctgct
     3781 tcacttcggc gaccgcctgt gccaactgac cggcaccggg cagccgtccc tgtccggcag
     3841 cttccttcca taccctggta accgcctcga ccgaagcgtc gagggcgccc gcggtgcgga
     3901 tacgccactg gtcgaaaagc cccaacactt cgaataactc gggtgacacg cggaccggta
     3961 catgggcagt acgctcccac cggctgaatt cgctgttcct attataggcg ctgagtatgg
     4021 ccgataaccg gccgatctca tgcattacgg ctttttgggc cttctccgct gctgccgggg
     4081 aagtagtgga cactttcagc tccatggagg tgccaatcac attctcatag tcggaaacat
     4141 actggcggga attcggaggg gcgcccgcat aaagaaatag gggcgccaga tacaggaaga
     4201 tcagcagccc gggcaggaat ttcatgcaat ttggttttac ggtccgtaaa aaatacaatt
     4261 tcccaatcgc catataatga tccgaaatgt taaaggttgc ataaaagagg tgaaaaaaaa
     4321 tcaaatcttg ttcggctttt ttagctaggt tgcccaatta aacagattcc cacagatgac
     4381 caaacgttgc cttgcctctg tcttttttat cgcgctattg gcccccggcc ggggcatcca
     4441 cgcccaggac aactatgaga tccaggtata tgcttcgccg acggtaggaa aggataccac
     4501 aatggtggaa ttgcacagta attacagttt tataggtgcg cccggagcgg atggtgtcgt
     4561 atccactaac catatgctgc gcgagaccat cgagatcacg catggcttta caccctggtt
     4621 cgagatggga ttttacattt tcaactgtat aggggatatg ggaaggacca attatgtcgg
     4681 cagccatctg cgtccgcgcg tcatggcgcc ggtaagttgg aactggccgg taggcgtcag
     4741 cgtttctttc gaaggagggt tcgtcaagga tggttatgac ccggatacct ggacgctcga
     4801 gatccggccg atcatcgaca aacagctggg accggtgtat ctggcactga atcccgtcgt
     4861 cgatctgacc tttgccggcg ccgataaagg tcgcggagtc ggtttcgctc ccgattttaa
     4921 attcagctat cagataggca aagtttgggc gcccggcatc gaatattatg gctctctcgg
     4981 accgttccgg aagttcgatc cgatcgacca gcaacagcat cagctgttca tcgccgtgga
     5041 cgcggatttc gacccgcgat gggaattcaa tgccggctat ggcttcggct ggggcatcga
     5101 taaaagcatc ttcaaggtaa tactcgggcg gcggttcggc ggcggtaaac atgcgcacac
     5161 cctcggtggg acgccgcaca cggtatttcg tttttagctt gccatccgca acacccatcc
     5221 caacatcgcg cctccgaaga tcacccaggc gctattcagc tttctccatc cgaaggtggc
     5281 cacaaacccc gctatggcga tcaggatcgt acgccagtcg gtaaccgtgt cgtgtccgaa
     5341 tcggatacac accacgccga tgagcgccat ggaggccaca ttcactccgt cgagaaaggt
     5401 agaaaagaaa gccgagcggc gcatcagcgg caacagcgga ttcaacgccg ctacaaacac
     5461 gaatgaaggc aggaaaacag ccagtgtcgc cagcgccgct ccacgaagtc cgcccatctg
     5521 ccacccgata aaggtcaccg aggaaaagac gggacccggc gtgaattgcc cgaccgcaat
     5581 agcatccacc aactgctgtc gcggcaggag tcctttcgcc accagctcgc tgtccaaaaa
     5641 ggcgaaaagt acatacccgc tgccataaag gatggctcct atctttagaa aggaaaaaaa
     5701 tatgcgaata ttggacgacg ttagtaccgg tgtctgtaga aacagccagg gaacgataac
     5761 cctcagatcc cccgcggacg cagcgccatt ctccacatcg aatagacgac acccgcgccg
     5821 aacaggatgg cgatctcgcc ttcgctccac cacaaccccg cggcaaatgc cgccacaagc
     5881 aataaccata gctggatatt cttcaacgcc cgacgagcca atggatatac cgcccctgcg
     5941 atgacggcga tgacggcagg tttgatgcca tagataagat cagccacctg gggcagacga
     6001 ccatatcgtt gatacaacca ggcgatgacg cccgtgatca ccaccgcggg aaggatgaaa
     6061 caggcgccgg cgatgagaag tcccttgcct ccgcctcttt cgcgaccgat atggatggcc
     6121 atttcggtgc tgttgggacc cgggatcaga ttcgtggcgc ccaacagatc tagaaaatgc
     6181 tggtgatcca tccaatgccg cttgtcgaca acttcttttt gcatgagcgc gatatgcgcc
     6241 gccggtcctc cgaatgcggt cagccccagc cgaatgaaaa gacgcaccaa atctccgggc
     6301 ggagtggccg gtgcaggtcc attttgggaa gtggcatttt ccatgtcggg acgggagtat
     6361 taacgcggct tttcaaacac ctgattgctg aaaggcaccg tggtcggcaa ctgtattacg
     6421 ttgaactcgt gtttggtcgc gcggtggcag ttattgcagg tggctgtcag gtaaacgaac
     6481 ccattcctga acgcgtcgcc atcagctctc gagaccgcat gcgagacact gtccagcgcg
     6541 ggctgtagca tgggcagcga ggcgacctcg gggcgatcgg tacaatattt tttgatatcg
     6601 tcgatggcct cctgcatctc gccgatctcg aaatccgcca atttccagtt ctccgtcttt
     6661 ccggcgaacc ataatttttc gtgatgaacc tggatgcccg acatgaactc tcccagaccg
     6721 ggagtataga cattccggag ctgttgttgc agcgtgtcga tctgctgttg caaaacggcg
     6781 gtattgtcgt ggggctggct acaagcgata aaaagcacga acacgccccc gatggagata
     6841 gatatctttt tcatggccct taaagataag aaatgtccgg atcggtcaat tcgatagctt
     6901 ttaagggcct gtgtaaggac ttacccaacg ctattgaaca ccgacatgga ggttatccag
     6961 acaagctccc acggcgacaa ccagctctcg cggccgcggc tcatcggcga tccatttacc
     7021 cctgtcgagc gacaggttgc cgaaatatac tttattcaat accacgtggt aactctgcgg
     7081 gaagccatcg tcatgcagct gctcggaggg agtcgcccat cctttgtact cgatgccttg
     7141 tccattgaat tctataggaa tgatttcttt tgccataata gtctccaatc tcaaaaaccg
     7201 ggcccacccc cgccgcgcac gtattacctc ctcctaacta ccagaaacac agcacctcgc
     7261 gcgggtacca tgaccctcac cccacccttc gcggacgcac caaaagcccg cagagccgcg
     7321 taatcggcag ggcccccggc ggccgccatg gtcgagtctc cattgacggc cactttccgc
     7381 gaaaattcgc cgttgtcgtc actgccttcg agactatacc agtaataacg gccgcgtgaa
     7441 aaatggggaa tttctatctc caccgtcgac gcatgtgtag acgtattgac cagtgccgcg
     7501 ccgatctcac cggagctgaa agacgaagca taggcccgga ggctggtgtc gccggccagg
     7561 tgcgagacaa caagcctgtc gccgagcata cgctgcaggt aatagagata atagaaagag
     7621 ggacgcggcg aatatcttct cacacccggc tcgtcaccgc tgctgaaaag gccatgatca
     7681 ttcccacccg cccaaccgtt gagcatatcc caacgcgcgg caagaccata tttattccgt
     7741 aaagcctccc ccatgacaat gatggagaac aacccgctga tattggacac ctgttgcttg
     7801 ctgccggagg cgaacatgtt ccattcgtcc atggctatag gctttgtggc ggcgccgttc
     7861 tcgcgcagac atttcagcac ataatccatc atgtcgccgg gaacctgaac cgcgtcatgc
     7921 aggatgtcgg cggcgcccgt atttgcgttc catggcgtga agtagttatg gacggcataa
     7981 aaatcgggca tgtcgccggc agcagggatc atcccggaat tccagtccct cgtgagcggc
     8041 ggatcgaaca gcttcggagg cgtttcatgc atgacggcgc cgatataaat ggtcgatccg
     8101 acctcttttg cagcgttgcg catcgagtcg gcaaagatcc tgaagtgttc gccataaaga
     8161 cgaccggtca gcagctgcgg ctgtccatcc ctgttggtcg ccgtgtcgat ccggtaaccc
     8221 agctcccagt taccgtaatt ttcgttgcct atctcccaat acctcgtccg tccatgatca
     8281 tagcgtaccc agtcggcggc aagatgggca gccgtcgcga cggggttggg accggtacca
     8341 taccgggcat acccataatt cacggtgatc agcccttcat tccccgtggc cgccagcaaa
     8401 tcgtaataat catccaccga agccccgaaa cggcccctgg gccgtccata gaaccaatgc
     8461 ggggccgtcc gttttcccct ggtatccaat acttccaccg gcagatcgga aggcatgcta
     8521 ccggggccgg aattccagaa ataaccatcg ctgccgctgc ccgcgggcca gcggatgata
     8581 tggctttgaa ggttcttcaa atggtccatc aatacgggtt gcgtaaccat ggtccccatc
     8641 caggtgtcgg cgttgtgacc gaattcggcc ggaggtatcc gcgtgatgac gctgtcgggg
     8701 tcgatgcgca aagtagcgct cgcccgccca gccacggcgc cctcgcgaaa tgcgggaacg
     8761 acaaaggtct tcggcttcca gtcatccata aaaaagccga tagtaggcgc tacctccggg
     8821 tcacggggat gcggagacgc gatcaacagg caacagaata agagcaaccc tttttgcata
     8881 gacggatatt ttatcgtttg gacaggcgat aggcgcgcat cggctgatca ttgacggcag
     8941 tcagcaggta ccggtccccg ccggcatgga tcaattttat atccctgacg tccccgtcca
     9001 gcaccagacc gtcggaagcc ggtggcatca cgcgccagcc gccccggccg tcgccctcca
     9061 gcaagaggcc ataagaggcg tcataacgtc cggacatcaa cgacgcttca tagtcattgc
     9121 ccgccaggat gaggtcggta aaaccgtccc cgttgacatc cccggctgca gtagcgttga
     9181 ccggcgctat ttgcgccatt gccgggaatg gatgaaaacg gaaatttcct ttcccgtcat
     9241 tccggaacca gccactcctc acttctttgc aatcgaggac cagggcgccc tgcatcattt
     9301 cgggagtaaa tatctcctcc atcggcgcag aagcatattt cgcgttgtca tcgaactttt
     9361 tcttgatcga aggcatctcc atggcccagt tgtccctgct gaccccggcc gatagccggt
     9421 atgttccgtc atccccgcgg atataatagc aaaacaccgg gtccatgatc ccattcccgt
     9481 cgaaatcttt ggcgatcaat tccgccggcc tgtcgctgct gatatgccac gggttgttgg
     9541 tccccaggtt gcccgcgacg atatcgggat gaccgtcatg atcgacatcc acgatctgaa
     9601 ggcttctcca gaatccggac aggtctttca gaccgctgcg cccggtgatc tcggtgagcg
     9661 tatggccatc attcgtaaaa atccggatcg gcatccaatc gcctgcgacg atgagatcgg
     9721 gtttgccatc tccatcgata tccgcccaga cggccgcatt gatcatgccg atatgtctca
     9781 gcgcggggca cacagcatcc gtcacatccg taaaccgtcc gtgatcgttg cgaaggatat
     9841 agctgtcggg cggcctgggg tatgagccga gcgataccct ccctccaatg aagatgtcca
     9901 tatcgccgtc cccgtccatg tcggcgacgg ccaccgcttt agccggcgtg cgcaccagcg
     9961 gcgaaaaggc aacgctgtcg agacggaaat tacctttccc atcgttgagg tacaaacgcg
    10021 gacagtaaaa agaggaatgg tcctcgaatt cgctgctacc gcccacgacc aggagatcgg
    10081 gatccttgtc cccgtccgca tcgaagaaca cgctctgcat atcctcttca tacttattgc
    10141 cggtgacgag atcccggccc gtgaagctgc cgtccctgtt ttgcaggtat agccgtccgg
    10201 actgtttgaa ggctcccccg atgaagaaat cctccagccc gtcgccgttg acgtcgcccg
    10261 tactgataaa agggccctcc tgcgagaatt tctgcaatag catgggctgg aacttgaaat
    10321 catagaagaa ggtctccttg tgacggaaat ccgttttaag cctccgcgtc gcgtcttcga
    10381 acagcggatc ggcgtcctgc actgccggcg gatcgaccac cgcgcttttc caatccaagg
    10441 tcaatatcgt atccactccc ggcctcccta gcacctgcat cctcccatcc ggccagacga
    10501 ccttcaacga atccggagcc gccgctccgc agcctatatg aagccggctg tctacggtcg
    10561 acaaaaaccc tctcacagga tattgcgtca cggtttgtac ctcaccgttg ccatatgccg
    10621 tcactgtcgc cccgatgccg tccctgttca gcgagtcgcc gctgagacgc aaggtcagat
    10681 aatgcgccgg acgcgaatca ttatggcgca cggtcgcggg agcattgata ttgttggtta
    10741 cgatgtccag ttctccgtca ttgtcgagat cggcatatat ggcgccgttg gcaatgcctt
    10801 tttcatggat ccccgcactg tctgaaatgt cttcaaagct gataccgtcc ttgttccggt
    10861 aaacataatt cctcagcgac agcggcccca tcgccgacag ccgctccatg aatttccggc
    10921 gctgaccgat ctcattttcg ggaataccgg ttgccagcac ggtgttatgc gcatattcga
    10981 gaaaatcgat attggtagga tcacgcccca acccgttggt gatgtgcata tccttccatc
    11041 cgtcattgtc gaaatcggcg atcagcacgc tccagctcca gtccgtctcc gcaataccgg
    11101 ccatctctcc gatctcgctg aaaaaaggct ccgtgcgacc gtccacccat ctggtgccgt
    11161 tgttcagctg cagcatattg tggatgaact gtggttcgta gcccttctcc atctccagct
    11221 ggtgacgctg ctcgctcaga aaggaataca tcattttctt ccggaaatta ttctcgggct
    11281 gcatatccag agtgacgatg tcggggagac cgtcgttatt gatatccgcc gcgtcgacgc
    11341 ccatgctgga atagctttga tgccgcacgg cagttgcgat gcaattggtg aaagtaccgt
    11401 tcccgttgtt cagccataag aggtcatttc ggatatagtc gttatcgaca tagaggtcgg
    11461 gatagccgtc gccgttgaag tcgcccgcaa tgaccccgag accgttgccg tcctcgacta
    11521 tacccgcctg acgggaaaca tccctgaaaa cgggatggtc cgttcccggc gctttgcctt
    11581 cattgtggaa cagcttgtcg gcggcaatat tggcggtgtc caccgatggg tcgcggatgt
    11641 tgttgggcag acggtcggcg agggtgtgac gtaccagata catgtccaac cgcccgtccc
    11701 tgtcatagtc gaaaaaaact gcctgtgtgg aatagcccgt atcggccagg ccataagccg
    11761 cggcttcctc cttgaaagta agatcatgct ggttgatgaa caggaggttc ctccggcggg
    11821 cgccgggcgc ctgtcccgat acgcacacat agatatccgg ccatccgtca ttattgatat
    11881 cgacgacgct tacgccggtg caccagaccc cggtacacac gccggccttt tgcgtgatat
    11941 cttcgaaatg catcccgccc ttattgagat agagtctcga gctgacctgg ttgcccgcga
    12001 aaaatagatc gggcagaccg tcccggttga aatcgcctac ccctacccct cctcccatgt
    12061 acgtatattc attgacgaac atgttcaggg tatcgttctc cgtgatattg ttggcgaacc
    12121 ctatacccgt cgaagaggcc ggcaacaagg taaaatgcag ccccctccta tcgtggacgg
    12181 ggttgcacga cagggcgcct ataaagaaga tacttatgac agttatacgg atcatctatt
    12241 tgccatccgc cacgaggtaa atgacgctca tgggaggtac ggctattttt atcgtcccat
    12301 tcagggtcgc cgcattggcg ctgatcgaac cgtagttcag cggtccgccc gtcgaagtag
    12361 aaggtccggc cccgttgaca tacacctgcc ccgagaacgg agcattgtcg gtcccaccgg
    12421 taagcacgaa ccaatggtac ttcttcccgg cggggaaatg ctggaagtcg atggagagca
    12481 catggttgaa attgcccgtg ttgatgatga ccgttcccgc ctgtccgctg ctgaaggtgg
    12541 acgaataggt cgtaagatcg ttattgccct gcacatgctt caaggtatca tagaccatcc
    12601 ggtcgccgaa cattcggtgg aagaaataca tatagtaaaa agccggtcgc ggattccaca
    12661 tcggtgcgcc gggttcgtcc ccgttgttga acatacccat atcgtctccc catggcgtgg
    12721 agccattggc cagatcccag cgactggctt caccaagtcc gctctttaga aaagagccga
    12781 gcgtcagggc ggcatgcatg ccggcgatat aggaaactgc ctgtttcgaa cccaccgcct
    12841 ggatattcca ttccgtcaga gcgatgggct tattggccac accggccccc tgcagctgct
    12901 gtttgatata ggtcatcgcg gtcggcgctt cggtggttcc cgtgcctagt atctgcggca
    12961 cggtggagtt ggctgcatat gcggtataat agtcgtgatc gatgaagaaa tccgccgtag
    13021 tgccggcatt cgccaatacg ccctggttcc aggtctgtac ggtgttgttg tcgtaggact
    13081 ggggtggcga gccatacacg atcgcgccga tatagatggt ggcgccgatg gattgcgccg
    13141 cggcgcgcat tgagtccgca aatacattga aatgcttacc gtacaaagcg ccggtcacgg
    13201 ttgcgggctg tccgtccttg tttttggaga catcgatctg gaagcccgct tcccagctgc
    13261 catagatctc attgccgatc tcccagaatt tggtcttccc gttgtcgtat cgcacccagt
    13321 ccgccgccag gtgggccgca gccgcaacgg ggttaggtcc tgtgccatat cgggcatagc
    13381 cataattgat tgtgatgata ccggtgctgt tcgtttgcgc aagcaggctg taataattgg
    13441 agagcgaaag cgtccagctc gccgcgtcgc ctccatacca gctgccgatg gaccccaaat
    13501 tgccggacgt aatgatgctg tcgggcgcat cggccggatg gggattggct gtcgtcccgt
    13561 tccagaaata agtatcgctg acactgcctg ccggtgcacg gatgatattc ggcgagaggt
    13621 ccttgatgta ttgcaccagg ttggcctccg taacgatctg acccatccag agattggagt
    13681 tgttaccgaa gatataaggt ggcgttttga cctgcacctt gttaacgtcg atggtcaggc
    13741 tatccgatac ggcggttgtc gcggcggaga ccatgttgtc cgaaggcgcg ttgaacgtcc
    13801 tctgagccca ggcgttgccg aagaatccga tggtaggcgc cagcgacgga tccgaaggat
    13861 tgtagctgga a
//
LOCUS       DASYSV010000600         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_892812, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000600 DASYSV010000000
VERSION     DASYSV010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892812"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VGQ51_18720"
     CDS             complement(<1..721)
                     /locus_tag="VGQ51_18720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9038681.1"
                     /translation="MEKKFAGFLCAFLFTVAIAGAQPRTLTLDSAVEMGVRNSKQLKR
                     SQNKIEESLVRLDQVKDDALPSAKVSFQYLHALMLSRMIAIPGVTKEPIKIPFDFPAW
                     MGTLSVTEPIFAGNRLKYARQSANLMIEMSRLDADKDKEDVIYLVINSYLNYDKILEN
                     QLIVARNMQDVQNKLDEITKYESQGLATQNDVLRYQLQKSQMELTEIELENNRKIANY
                     NMNILLGLPDSTDIILPPLDYK"
     gene            complement(771..1427)
                     /locus_tag="VGQ51_18725"
     CDS             complement(771..1427)
                     /locus_tag="VGQ51_18725"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV9038682.1"
                     /translation="MSDKREHILIEAEKLFGERGFDGTSVRDIAQNAGVNLAMISYYF
                     GSKEKLLESLVVYRAEYAYGILEELNKDQSLSPWDKIDRLIDFYVDRILNNLRFHNIM
                     YQESTAVRSEEIKQRIFAIKMRNLDQITKIITDGQQKRLFRQVDIPMTVGTLMGTISY
                     YTQSRTYSCNILAIREDTDDESYRATLSPRLKAHLKQVMRAHLDIRNDSGQQPYQPQG
                     "
     gene            complement(1592..2578)
                     /locus_tag="VGQ51_18730"
     CDS             complement(1592..2578)
                     /locus_tag="VGQ51_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV9038683.1"
                     /translation="MEYRRLGRSGLQMSVLSFGSWVTFHGQIEDKNADELMSIAYEQG
                     INFFDNAETYADGESEKMMGRVLQRKSWDRTSYIVSSKAYFGSRKRLLPNMTGLSRKH
                     LVEACNEALDRLRLDYLDLYFCHRPDPNTPMDEVVWTMHNLVQQGKILYWGTSQWSAA
                     EIMEAHSVAQQYRLIGPVMEQPQYNMFERYKMELEYPPIFRNVGMGTTIFSPLAAGFL
                     TGKYQGGIPEGSRLSMEGFDWLKRRWLKEDRIGKVEQLKTIAARLETSLPALAIAWTI
                     VNPNVTSAILGATKKDQLMENLKALDVVPRLTPDVMAGIEEILQNKPHFDMA"
     gene            complement(2589..>2870)
                     /gene="lptC"
                     /locus_tag="VGQ51_18735"
     CDS             complement(2589..>2870)
                     /gene="lptC"
                     /locus_tag="VGQ51_18735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEV9038684.1"
                     /translation="DAKYGKYLQNQEKVFLRDSVVVKNILKGDTLRCKTLWWDQHTER
                     FTTDDSVRIYTPDKILYGTGMEADQNFRWYTIRHMTGMVLNSGGNIPKQ"
BASE COUNT          612 a          756 c          749 g          753 t
ORIGIN      
        1 tcttatagtc cagcggaggg aggatgatat ccgtcgagtc cggcagaccc agcaggatat
       61 tcatattata gttggcgatc tttcggttgt tctccagctc gatctcggtg agctccatct
      121 gcgatttctg cagctggtaa cgaagtacat cgttctgcgt ggccaggccc tggctttcat
      181 atttggtgat ctcatcgagc ttgttttgca catcctgcat atttcgcgcg acgatgagct
      241 ggttctcgag gatcttgtcg tagttcaggt acgaattgat cacgagatag atgacatctt
      301 ccttatcctt atcggcatcg agacggctca tttctatcat cagattggcc gactggcggg
      361 catatttgag cctgttgccc gcgaagatgg gttcggttac ggagagtgtt cccatccagg
      421 cggggaaatc gaatgggatc ttgatcggct ccttcgtgac cccgggaata gcgatcatcc
      481 gggacagcat cagtgcgtgc aaatattgaa agctgacctt ggcactcggc agtgcgtcgt
      541 ccttcacctg gtcgagccgg accagggatt cctcgatctt attctgcgaa cgtttcagct
      601 gtttgctgtt ccggaccccc atttccaccg ccgaatccag cgtgagcgtt cggggttgag
      661 cgcccgctat agcgacggtg aaaaggaagg cgcataaaaa tcctgcaaac tttttttcca
      721 tgttggtctg gtatttagtc tttcggttca ataaattttg gtatcggaaa tcatccctgc
      781 ggctggtatg gctgctgtcc ggaatcatta cggatatcca gatgagccct catcacttgt
      841 ttgaggtgcg ccttcagacg cggggaaagt gttgcgcgat acgattcgtc gtcggtgtct
      901 tctcttatgg caaggatgtt acaactgtag gtccgggatt gggtatagta agagatcgtt
      961 cccatcagcg tccccaccgt catggggatg tccacctgcc ggaacagacg tttttgctga
     1021 ccgtcggtga tgatcttggt gatctggtcg agattgcgca ttttgatcgc gaagatcctc
     1081 tgcttgatct cttccgaccg gacggcggtg gattcctggt acatgatgtt atggaagcgc
     1141 agattgttca ggatccggtc gacatagaaa tcgatcagcc ggtcgatctt gtcccaggga
     1201 ctgagagact gatccttatt cagctcttcc aggataccat aggcatattc ggcacggtat
     1261 acgaccagcg actccagcaa tttctctttg gagccgaaat aataagagat catcgccagg
     1321 ttgacccctg cgttttgtgc gatgtcccgg accgaagtcc catcaaaccc cctttctcca
     1381 aaaagcttct cggcttcgat caggatatgt tctcttttat ctgacattat cgttacgaat
     1441 tgacggcaca aaattaaaca aacgtttgaa ttaaacaaac gtttaattga attaatttaa
     1501 caaaaaaccc cgcgggaggc gggtgaggaa gctgcgggag gcagggtgaa ggaagccgca
     1561 cgttgcagct gagccgttaa tgcattgatt attaagccat atcgaaatgc ggtttgtttt
     1621 gcaggatctc ctcgatccct gccatcacgt ctggcgtcag cctcggcacc acgtccaaag
     1681 ccttcaggtt ctccatcaac tggtcttttt tggtcgcacc cagaatggcc gaagtcacat
     1741 tcggattgac gatggtccac gcgatggcca gggcaggcag agaggtttca agacgggcgg
     1801 caatcgtttt cagctgttcc accttgccga tcctgtcttc tttcagccaa cgccgtttca
     1861 gccaatcgaa gccttccatc gagagacggg agccttcggg gatgccgccc tggtatttgc
     1921 ctgtgaggaa ccccgccgcc agcgggctga agatggtcgt acccatgccc acgttccgga
     1981 agatgggcgg gtactcgagc tccatcttat agcgctcgaa catattgtat tggggttgct
     2041 ccatcaccgg cccgataagg cggtattgct gcgccacgct atgggcctcc atgatctcgg
     2101 cggcgctcca ttgactcgta ccccaataga gtatcttgcc ttgctgtacg aggttgtgca
     2161 tggtccagac gacttcatcc atgggcgtat tcggatcggg acgatgacag aaataaagat
     2221 cgagataatc cagccggaga cggtccaggg cctcgttgca ggcttcgaca agatgtttac
     2281 ggcttaaacc cgtcatattc gggagcagcc gcttccggga gccgaaatat gctttcgaag
     2341 agacgatata agaggtccgg tcccagctct tccgctggag cacgcggccc atcatcttct
     2401 cgctctcacc gtccgcatag gtctccgcgt tgtcgaaaaa attgatcccc tgctcatatg
     2461 cgatgctcat gagctcgtcg gcgtttttgt cttctatctg cccgtgaaag gtaacccagc
     2521 tgccgaagga caggacgctc atctgcagac ccgacctccc taaacgacgg tattccatat
     2581 tgtttatttt actgtttcgg gatattccca ccggagttga gcaccattcc tgtcatatgc
     2641 cggatcgtgt accagcggaa attctggtcc gcctccatgc ccgttccata aaggatcttg
     2701 tccggcgtat agatgcgtac cgagtcatcc gtcgtgaaac gctcggtatg ctgatcccac
     2761 caaagggtct tgcagcggag cgtatcgccc ttcaggatat tcttcactac caccgaatcc
     2821 cgaagaaata ccttctcctg gttctgcagg tactttccgt atttcgcgtc
//
LOCUS       DASYSV010000601         3546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2044945, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000601 DASYSV010000000
VERSION     DASYSV010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044945"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VGQ51_18740"
     CDS             complement(<1..646)
                     /locus_tag="VGQ51_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038685.1"
                     /translation="MIRCWKHTIVGLLSLFLIGFLACQKTATEKKVELTPLQTLINTD
                     TTLSLYHLMLLRANDIGLMKDTTASLLVPRNEVLVAAGYPEIIIDSMSSALADRIVRY
                     NYLPSGISTDSGGATPTRLGAPLYIQDNGNGTLVLNGTGMAGDKPASVGQAAVYWLTS
                     MVPPAADSLPDILSTDSSLTYLNAVFARTNIYDSLLQTGSFTLLAPVNSAFQAAG"
     gene            complement(655..1737)
                     /locus_tag="VGQ51_18745"
     CDS             complement(655..1737)
                     /locus_tag="VGQ51_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV9038686.1"
                     /translation="MSANKFRNFKMVGYVIYGRGSFNQLDEILAPHRKGDAPMVFLLD
                     HYFENKPLAKRIPLRGKDKLIFVDVTYEPKTSYVDKLAGGLKQESGTVSGIIGIGGGS
                     AMDLAKAVSLMMTNPGSSADYQGWDLVKFPGVYKVGIPTLSGTGAEVSRTTVLTGPTK
                     KLGMNSDFTPFDQIVLDPELTADAPVNQRFYTGMDCYIHCIESLEGTYLNEFSKSYGE
                     KALQLCQEVYVRKLGWDADSDDKLMMASYAGGMSIAYSQVGVAHAVSYGLAYLLGTKH
                     GIGNCIVFDKLEEFYPDGVREFKVMVEKNKIDIPQHITKGLTDEQFNTMIDVSLVMKP
                     LWENALGKDWERIMTRDRLRALYEKL"
     gene            complement(1824..2732)
                     /locus_tag="VGQ51_18750"
     CDS             complement(1824..2732)
                     /locus_tag="VGQ51_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV9038687.1"
                     /translation="MKQPIWGIDLGGTKVEGVILESIDNPAAIVRTRVDTEATRGYDH
                     VIGQIGKLVAEMGRQSGLKPTAIGIGTPGVLDPTLQVMKNCNTVVLNGKPLKKDLESA
                     LRLKIAMANDANCFALAETHWGIVHHSAPGARLVFGIIMGTGVGGGIVIDGKIRDGRH
                     GIAGEWGHNFLDESGGPCYCGKTGCVETVISGPATERYYHSITGERLKLKDIVARDGS
                     DPAATASIDRLCHFFGKGVSVITNLLDPDVIVVGGGVGNIQALYSRGLESLNKHIFNN
                     RVEVPLLKPSLGDSAGVFGAAALVAF"
     gene            complement(2799..3524)
                     /gene="kdsB"
                     /locus_tag="VGQ51_18755"
     CDS             complement(2799..3524)
                     /gene="kdsB"
                     /locus_tag="VGQ51_18755"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEV9038688.1"
                     /translation="MRIIAMIPARYAATRFPAKLMQRLGEKTVIRHTYDNTVATGLFD
                     EVLVVTDSDIIYREITAHGGKAKMSVRTHESGSDRIAEAVSDMDVDIVVNVQGDEPFV
                     RREPLESLLEVFKGDAGKQVQVASLVQVLKEQRFIDDPNYVKVALDKYNNALFFSRSP
                     IPYPRDPSASPVYYEHIGVYAFRKQALLQFTGWPVSPLEAVEKIECLRYLENNVPMRM
                     VVTRYMGIEIDTPEDLERAARLL"
BASE COUNT          772 a          977 c          954 g          843 t
ORIGIN      
        1 atcctgccgc ctggaaggcg ctgttcaccg gggctaacag ggtaaaactg cccgtttgca
       61 acagcgagtc atagatattg gtccttgcga acaccgcatt cagataggta agactgctgt
      121 cggtgctcag aatatccggg agcgaatccg ccgcaggagg caccatcgag gtcagccaat
      181 atacggcagc ctgcccgacg gaagcaggtt tgtcaccggc catacctgta ccattcaaca
      241 ccagcgtccc atttccgttg tcctggatgt ataggggagc gccgagacgg gtgggggtgg
      301 cgccgccgct gtcagtagaa atgccggaag gaagatagtt gtagcggacg atcctgtcgg
      361 ccagggccga gctcatcgaa tcgatgatga tctcgggata cccggcagct accaaaacct
      421 cattccgcgg aacgagcagc gaagcggtcg tatccttcat caggccgata tcattggccc
      481 tcaacagcat caggtggtaa agcgaaaggg tcgtgtcggt attgatcagt gtttgaagag
      541 gggtaagctc aacctttttt tccgtcgctg tcttctgaca ggcgagaaag ccaatcagaa
      601 aaaggctaag caggccgact atcgtatgct tccagcagcg gatcattggg tattttacag
      661 cttttcgtac aaggcccgca gccggtcacg ggtcataatc ctttcccagt cttttccgag
      721 ggcgttctcc cacagcggct tcatcaccag actaacgtcg atcatagtgt tgaattgttc
      781 atcggtgagc ccttttgtaa tatgttgagg gatgtcgatc ttattttttt ccaccatcac
      841 cttgaattcc cggaccccgt cggggtagaa ttcctccagc ttgtcgaaaa cgatacagtt
      901 gccaatgcca tgtttggtac ccagaagata ggccaggcca taactgaccg catgggcgac
      961 acctacctgg gaataagcga tgctcatacc gccggcatac gaagccatca tcagtttgtc
     1021 gtcgctgtcc gcgtcccagc ctagtttcct gacatagacc tcctggcata gctggagggc
     1081 tttttcgcca taggatttgc tgaattcatt gagataggtg ccctccaggc tttcgataca
     1141 atggatatag cagtccatac cggtataaaa gcgttgattg accggggcat cggcggtgag
     1201 ttctggatcc agcacgatct ggtcgaaagg agtgaaatcg gaattcatgc ctaatttctt
     1261 ggtcgggccg gtcagaacgg tggtacggga tacttctgcc cccgtcccgc ttagggtcgg
     1321 tatcccgacc ttataaacgc ctggaaattt gacgagatcc caaccctggt agtccgccga
     1381 ggagccggga ttggtcatca tgagggaaac ggccttggcc aggtccattg ccgaaccgcc
     1441 gcctattccg atgataccgc tgacggtccc ggattcctgt ttcagtccgc cggccagctt
     1501 gtcgacataa gacgttttgg gttcatatgt cacgtcgacg aatatcagtt tgtcttttcc
     1561 ccggagcggg atgcgtttcg ccaggggttt attttcaaaa tagtgatcca acaggaagac
     1621 catgggggcg tcccctttcc tgtggggagc gagtatttcg tccagctggt tgaaactgcc
     1681 gcggccatag atgacgtagc ctaccatctt gaaattccgg aacttattcg cgctcatatt
     1741 ggtcttttta atggtgctca tgaatgcggg gggaagcgca tttcatgcat ttacgattga
     1801 gcggcaaagg taggaaatag tggttagaat gccaccagcg cggcggctcc gaacacaccg
     1861 gcggagtcgc cgagcgaagg tttcagcagc ggcacttcca cgcgattatt gaagatatgt
     1921 ttgttgagcg attccagtcc ccgcgaatac aacgcctgaa tattgcctac acctccaccc
     1981 acgacgatga catcgggatc cagcaggttg gtgatgacgg aaacaccttt gccgaagaaa
     2041 tgacagagac ggtcgatact cgccgtcgcc gccggatcgc ttccatccct ggcgacgatg
     2101 tcttttaact ttagacgctc ccccgtgatc gaatgataat atcgttcggt cgccggtccg
     2161 gatatcacag tctcgacaca gcctgtctta ccgcaatagc agggaccgcc cgattcatcc
     2221 aggaaattat ggccccactc gccggcgatg ccgtgccggc cgtcccggat cttcccgtca
     2281 atgactattc cccctccgac gcctgtaccc atgatgatgc cgaataccag ccgggctccg
     2341 ggagcggaat gatgaacgat accccagtgt gtttccgcaa gtgcgaaaca gttggcgtcg
     2401 ttggccatcg ctatctttag ccgcaaggcg gattcaaggt cttttttcag cggctttccg
     2461 ttgagtacaa ccgtattgca gttcttcatg acctgcagcg tggggtccag gacaccgggc
     2521 gtgccgatgc cgattgcagt cggcttcaga cccgattgac ggcccatctc cgccaccaac
     2581 ttccctatct gaccgatgac atgatcgtat ccccgggttg cctccgtatc gacgcgggtc
     2641 cggacaattg cggcagggtt gtcgatggat tcgaggatga cgccttcgac tttcgttcct
     2701 cccagatcaa ttccccaaat tggttgcttc atgtgcgcca agataagaat tccccttttt
     2761 agcagattgc aaaaatttgt ggatcgcggc cagcgcgctt aaagcaaccg cgccgcccgt
     2821 tcgaggtctt ccggcgtatc gatctcaata cccatatacc gcgtcaccac cattcgcatc
     2881 gggacattgt tctcgaggta acgcaggcat tcgatcttct ctaccgcctc gagcgggctg
     2941 acgggccagc cggtaaattg cagcaaggcc tgtttgcgaa aggcatatac gccaatatgt
     3001 tcataataaa cgggtgaagc cgaagggtct ctcgggtagg ggatggggct ccggctgaag
     3061 aacagcgcgt tgttgtactt gtccagcgcc accttcacat aatttggatc gtcgatgaac
     3121 cgctgttctt tcagcacctg taccaatgat gcgacctgca cctgttttcc ggcatctcct
     3181 ttgaacacct ccagcaaact ttccaatggc tcgcggcgga cgaacggctc gtctccctgt
     3241 acattgacga cgatatccac gtccatatcc gagacggctt cggcgatgcg gtcgctgccg
     3301 ctctcatgcg tgcgaacgct cattttggct tttccgccat gcgcggtgat ctcccggtaa
     3361 atgatatcgc tgtcggtgac gacgagcacc tcgtcgaaca gaccggtggc gacggtatta
     3421 tcgtaagtat gacggatgac ggtcttttcg cccagtcgtt gcatcagctt cgcggggaaa
     3481 cgggtggccg cgtatctcgc agggatcatg gcaataatgc gcatagaaaa attttggcga
     3541 aattac
//
LOCUS       DASYSV010000602        19339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1405279, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000602 DASYSV010000000
VERSION     DASYSV010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 19339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19339
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405279"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..4796)
                     /locus_tag="VGQ51_18760"
     CDS             complement(129..4796)
                     /locus_tag="VGQ51_18760"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEV9038689.1"
                     /translation="MKELLAILVCSGALRLAAQRPDTTLFTLLGEKQTGISFSNNIRE
                     DDSLNVFSYEYLYNGAGIGIADLNGDGLDDIFFSGNTGPDKLFLNKGNFRFEDVTRQA
                     HLSGNGTWSTGVSIADVNGDGLLDIYVCHAGKYDDSTRLSNELFICQGIKNGVPVYKD
                     MAAAYGLDAPGTQSTQAVFFDYDKDGDLDMFLLNHSNHTYNPYLNTRQTRATPDMHFG
                     NRLFRQDRDKEGHIHFTDVTLQAGIINNPLNFGLGVVVSDVNGDGWPDIYTTSDYTER
                     DCFYINNHDGTFTESLEKSFTHISKYSMGLDIADYNNDGRPDVMTLDMLPEDNHRQKL
                     LKGPDEYDEYHLLLDSGYYHQQMRNMLHLNEGEDEKGDLRFSEIGQLAGVSNTDWSWS
                     PLLADFDNDGKKDLFISNGYLRDFTDLDFLKYTVPDAQLAAAREGHTNFRTYDLVKKM
                     PSNKLSSYIFHNNGDLTFSNMTVGWGLSHPAVSNAAAYADLDNDGDLDLVVCTNDEPV
                     MVYRNNGSQLKKDHYIRLRLTGRGLNTRAYGARAVLTTSDGSSQYEELYPVRGFQSSM
                     APELFFGWPSGTDARKITITWPDDSVTVLDRLEADRTLDVRENGPAAPVRIAEPNRLF
                     TDVTASSGISFRHRENDFIDFKDEPLLPYQLSREGPAIAKGDVNGDGLEDIFLGGAIG
                     QTGQLFLQTGDGHFVRAPSQPWIADSVSEQVNALFFDADGDGDMDLYVVSGGNEYSEG
                     PGYQDHLYINDGKGNFTLAPEGALPVMASSKFAIAAGDFNHDGKPDLFVGGRGVPGSF
                     PLPSRSWLLRNDSRDGAIRFTDVTDEVCPELRVPGMVKAAVFTGLDDPGYPDLLIAGD
                     WMPVRLFRNDKGRLSDVSGSAGLTHLNGMWSSITPADVDGDGKIDFILGNCGLNNQFK
                     VSPSQPMTLYVSDFDGNGSLDPILCYYIGGKSYPMASRDELLDQIVTLRKKFNSYKAY
                     ADATITDIFPPEKLAKATVLQCDQLASGVLYNRGGGRFSFSPLPTAAQFSKIYGVVVD
                     DFDGDGKKDILVSGNFFPYRTQLGRCDAGLGMLLRGPDLQPVDNALSGAYIGGDVRGM
                     VEVKDRVGDRLLVVAKNNDAVQVLKVNGQPRPVAQAGGTRAFVRRIVAPGPIADDAEP
                     LRRAQAALTDIMVHDIFSPPVASRIYLYSNIAAYETLVKAHGHDYISLYGQVKGFPDI
                     PAPEKKIDFPLAAVYAYLLVGRRMIFSDSVMQDSIRAILGSMQKPAAAEYQASLKYGQ
                     TVASLIIAWSDKDRYTETRRLPRYRILRQPGKWIPTPPVYMSAVEPYWNRIRPVTLDS
                     AGQFKPAKAPPFSKDTGSYFYHQAYDVFRTVRALTDEQKNIANFWDCNPFAVTMEGHV
                     GYSTKKISPGGHWISIVSTVSRQRHLDMMETAGAYTITSIAIFDAIISCWDEKYRSNV
                     IRPETYIDSYIDEGWRPILQTPPFPEYTSGHSIISTVAAVVLTKWFGDHCAFTDDTEL
                     EFGLPERHFDSFRQAAGEAAISRLYGGIHYRAAIEAGVSSGDHIGEWVLQKINLKRSY
                     AAK"
     gene            complement(4793..6118)
                     /locus_tag="VGQ51_18765"
     CDS             complement(4793..6118)
                     /locus_tag="VGQ51_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV9038690.1"
                     /translation="MKRIFLNIAFFLTLTGPKPSFAQSSHPTFMDVAPIIRSKCATCH
                     KPGQTGPFSLITYEDVAKRVSFIRDVVQKGYMPPWKADNNYVHFANDRSLTVKEKTLL
                     LQWIDNKAPRGDGPDTAGLKEASIPGTSYFRKPDLVLKAKDSFRLTGDNIERYIIFKI
                     PFELKDSANVEAIEFFCSNKKLIHHANYAIHSVPDTNIDIYHTDAMLNLTEDDRRKFD
                     QYQPYRKTITYYGGWIPGASYEHFPKEFGWVMPRRGVILLTVHYAPSATDETSICGVN
                     IFFKKTPVTRVMKVVSFGSGGIGQNQIEPIFYIKAGKVETFHLEVTNPSQDQSLLYVW
                     PHMHLLGKEYKAYITTPAGDTIRLVHIPNWDFRWQEIYWFRHPIKVVQGSVIHIEGTY
                     DNTTDNPFNPNNPPKNVFSMGDMKTTDEMMTLMMGFLPYKEGDENIDLP"
     gene            complement(6120..6632)
                     /locus_tag="VGQ51_18770"
     CDS             complement(6120..6632)
                     /locus_tag="VGQ51_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEV9038691.1"
                     /translation="MKKLLLPLFVSCVLVFSGAWAQPRTAPLRVVIFLSPEGPLCQNY
                     SLPLNPLFKKYADKVQFTGVVPGKAYSAGDVNAFTQKYHIAFPVYIDSAKTMSTSLKA
                     TVTPEVFLLGPDKKVIYHGSIDNWIKDLGMQSARPTVFYLRDAIDQSLAHQPVRVPYN
                     KPVGCLINDY"
     gene            complement(6696..8309)
                     /locus_tag="VGQ51_18775"
     CDS             complement(6696..8309)
                     /locus_tag="VGQ51_18775"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEV9038692.1"
                     /translation="MNKKKYIVSLSIACLAMGACKKSAFVETNLNPTTLYAVDPADQF
                     LAAAAGCQDDFEYFYDVYRAENNWLQYTTSPAGNTPGFARETGNFNERYSKIFYGRVG
                     TYLSDMTYLVSQMKPGDQAKRAYQLSIGAIFKAYYAFYVSDINGDIPYSQAFQARYGG
                     TLTPVYDRQQMLFDTLDTQIKAAVKVLETAQTATQVPMGAKDPFFPTSISGNAALEAA
                     EWAKIGNAVRLKMAMRLMKRDPTRMKTIVTEVLADASQMASTDDTWALYAGPAFANGT
                     GNYNPVGFCAGKPVVDFMNLTGDPREKIFYRPALRTGTFVGAPTSPDDVAPNAQLFLH
                     SDSAFSQLQHRLFAPNYDEGDGNGSGTGVAFFPLMTYAEYCFIRAELGNRGVTGDDAA
                     TWYNKGVTASITYYDQRAQAAGIAGYTKVTASDITTYLTKVAYVPATGLDQIVCQAYL
                     DFFRQPSEAWAWWKRTGFPNTTSTIKWEQPTASGTVLTLPRRAAITVLLPTDRNYENQ
                     QAAFKNMTSDGNWGTGPNDFSGRVWWDMP"
     gene            complement(8336..11683)
                     /locus_tag="VGQ51_18780"
     CDS             complement(8336..11683)
                     /locus_tag="VGQ51_18780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9038693.1"
                     /translation="MRKLKAHLLLFMLTCMWSAALLGQTRISVSGNVRDSVGNPLSGI
                     SVTVKGTKSGTSTDALGNFKIGAQRGEHLVFSGIGFLSQQIEVTGGTITLAMHNDPKS
                     LNEVVVTGFGQRKNLRKVPYSVTEIKGDEITQANNANFVDALQGKVAGVFVGQGAGGP
                     SSSAKIRIRGNASLSSNTQPLVVIDGILIEPGTTGADSWGQNRDFGNIIKDLNPDDYE
                     SIDVLKGSAATALYGSRGLNGVLLITTKKGRARKDLGITFTHTESFDHAYKLPDYQNT
                     YGGGIKPTFDKDAQGNDIVDPLNASIYNTPDANQSFGPKFDGHMVKDIDGRMVPWVAN
                     KPLDFFQTGKYINTNLAIEGGNESSTYRFSVSDLQNNSIMPNNKLQRDAFSLRATHKI
                     GSLITLDASVSYTISTIKNPIWQGGNFSPLFAFTYFMPRNAPIDWWQHNYIDTVNGGQ
                     KTGPSGDPYYLAHTMWNYYQDNSTQHENNLLADLDLTIKLAPGLSALVRSNVNTYNFY
                     SEEDVNGSGKGFSGGSYTLIKGSYNNIRVQGLLNYTKELGEDFSLNASAGGETYRRLG
                     GQRIQLNTNGGLLAPGLYTITNSVSPASIQVNNTDAPYSSYRNDAVYVMGDLTWKNML
                     TANATLRNDWVSSLTYKDGHGKDYATYPAVGLAWTFTELPFFKNSNSILSFGKLRASL
                     GWSGSYPQAYYTNTAGFYSPNGTFNNSGNGTQQLFTFQGSNLGNPNLVAERARELELG
                     ADIRFFNNRLGFDVAWYKKNSFDQILNLNTPVESGASSQAINAGNIQNEGVEILMTAT
                     PVQLRNFTWNSTINFTRNRNQVISLAPGVKSYQLELAFGADEQAVAEAGKPYGTVVTG
                     YGYAIYNGKNSAATGKKVLGLAPYGTTGGYYSYLRSQDYDGSTKELGTIMENWLGSTT
                     QSFRYKSFNLSVQIDAKYGGLMASASHQYGSAGGSFKNSLHGRDAASGGLAYTDANGS
                     HNDGIIPDGVFNDGIVIKGVDVSGMSYADAVKANLVTPVPAYAYYGNLSQWSSGIREY
                     STFDNSWIAVREVTLAYDLPASIYTKVKFSSLRVAVTGRNLGYLYKTAKDGINPEGFY
                     NNNSAGFAEYGGLPYTRSLGFSVKAGF"
     gene            complement(11764..12870)
                     /locus_tag="VGQ51_18785"
     CDS             complement(11764..12870)
                     /locus_tag="VGQ51_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV9038694.1"
                     /translation="MTTKMVNELIRDNDIMETGYAPSTGGRRPQMYSLRPDRMYTLAV
                     AMDQLFTRIVIMDMQRQPVTAAEKFELHLAKNPRALETLAEKIDEHIHRSGIKKDRLA
                     GIGIGMPGFVDSRNGINYSFMGAGERSVSQYIMERVGVRTYIENDSSLIALAELSFGA
                     AYHKKSAMVINLSWGIGLGLILNGELFRGGNGFAGEFSHLPLFSNNKLCSCGKSGCLE
                     TEASLLVMVEKAKEGIAGGRVTMLTQQQLADNPDQASLAVISAAGKGDKFAVELLSES
                     GYNIGRGVAILIHLLNPETIVLGGRGAAAGKIWQTAIQQALNEHCIPRLSANTTIEVS
                     ELGLDAELIGAAALVMENYVKGSIKKGASAVMAR"
     gene            complement(13150..14511)
                     /locus_tag="VGQ51_18790"
     CDS             complement(13150..14511)
                     /locus_tag="VGQ51_18790"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEV9038695.1"
                     /translation="MDQLYKLNVREFGYDFLWGVAMAAVQNEGAWNSYGKGPSIWDTF
                     SRRQRKIKGGGRPTVACDFYHRYKDDLLLAKALGFSVFRFSLSWARVLPEGVGRVNKE
                     GVLFYHKVIEECHKLGLTPYVTLYHWDLPVTLEKEGGWTSHLMLRWFSRWATLCATEF
                     GPAVKDWIVLNEPFGFTSLGYMLGRHAPGRIGLSNFLPAIHHAALAQAEGGRIIRQLV
                     PGAHIGTSFSCSEVVPYTNSPEDIQAARRLDILMNRLFIEPALGRGYPREDFKFLERL
                     ELHNRSWKYTERMQFNFDFIGIQNYFPIVVRYNPLIPIVQASEVKASSRGVPYTAMGW
                     EINPDSFYRILKRFWLYGGVKEIIVTESGAAFKDEVVGGMVDDKARIDYYQQYLLALL
                     RARKEGVNIKGFFAWTLMDNFEWAEGYRARFGLIHVDFATQLRTIKSSGYWFRDWLTD
                     KKS"
     gene            complement(14514..15248)
                     /locus_tag="VGQ51_18795"
     CDS             complement(14514..15248)
                     /locus_tag="VGQ51_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038696.1"
                     /translation="MKIILTIAVVLIGSVFARAQKVVDITHSDADVVGQQMINGLVGG
                     QVITPVTYVKLTEGSPWFSDEWADGALLMTGGKVIEHLRLKLDLLQKEIHYKNADGQE
                     MIATTPIRAVTLSSPAGTITFVPGTPWRGIDKALDGAWLQVLVNDKVSLLHEIRKKMA
                     DNTPYGGSTVERTITDVHFFYLQINGKFLRIRSWSDLPALLGDKPQAVAAFVRDNHLK
                     GRTPDEYAQVVTYYNTLIDSQKVTKD"
     gene            complement(15245..15964)
                     /locus_tag="VGQ51_18800"
     CDS             complement(15245..15964)
                     /locus_tag="VGQ51_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038697.1"
                     /translation="MTAKLLLTAAVLLIGGFSTGAQDLGLYTFRDGDVIAQDVVNSLI
                     NGQASKPNEYAKVVEGTPFFRDEWSDGALLLPGGKVIEHLRLKLDLLQKEIHYINADS
                     QEMILTTPVRAVKLAGSTGPLIFISGTPWRSVERALDGAWVQVLVNDTVSLLHEIRKK
                     QIEQRTFGGTAAERTISDDDVYYARMNGQLLPVRSWSDLPAMFGDKKESLTKFTRDHH
                     LKGRTPDEYAQVVAYYNTLIK"
     gene            complement(15993..16877)
                     /gene="miaA"
                     /locus_tag="VGQ51_18805"
     CDS             complement(15993..16877)
                     /gene="miaA"
                     /locus_tag="VGQ51_18805"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV9038698.1"
                     /translation="MQQPTVIIIVGPTAVGKTAAAIRLARHLGTRIISADSRQCYKEL
                     DIGVAKPTPEELRLVPHYFINSHSITEEVNAATFEELALQWTSEIFRKSPYAVMVGGT
                     GLYIRAFTDGLDAIPPVDQGIRTHIRHHYEEKGLDWLQEEVRKYDPDFYSAGEILNPQ
                     RLMRALEVRMSTGQSILSFRTKQRPQRPFGIRTIGLQLPKGELHRRIDQRVDAMIRAG
                     LTKEVESLVAYRSHNALQTVGYRELFEWMDGKVSLESAIAAIKTSTRQYAKRQMTWFH
                     RDLSVRWTDPGTALETLL"
     gene            complement(16890..17474)
                     /locus_tag="VGQ51_18810"
     CDS             complement(16890..17474)
                     /locus_tag="VGQ51_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038699.1"
                     /translation="MAILKFRVYLEEDDSIYRDVAIRHTQSFLLLHQVILKSYEFDSK
                     HQATFYRSNDHWQRGRQISLELYEKNYPNKEPLLMEATTIGSEIRDPNQKFIYVYDFA
                     KNWSFLVELINVSKEEGSKVDYPVVVKTEGIAPSQYGTKGLVGDKLTEMEEKYDLTKG
                     TEGFGVEGESEEGESEEEGEGEGEGEAGAQEDTL"
     gene            complement(17578..18996)
                     /locus_tag="VGQ51_18815"
     CDS             complement(17578..18996)
                     /locus_tag="VGQ51_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV9038700.1"
                     /translation="MDIQCTDKELEVFEKIARAAEAMGTPAYLIGGFVRDKLIGRPTK
                     DADIVCVGDGISLAHKVADTLHPRPNVSYFKNFGTAQIKWSDWEIEFVGARRESYRDH
                     TRKPDVEPGTLEDDQLRRDFTINAMAISLNKADYGNLIDPFGGIDDLKKQTIRTPTEP
                     LKTFSDDPLRMLRGIRFATQLGFAIDDDTWKAIVRDCPRIKIVSQERITDELNKIVMA
                     AKPSIGFDLLYRSGLLKTIFPQMVDLSGAEYIDGMGHKDNFYHTLQVLDNISRNTDDL
                     WLRWAAILHDIGKPATKKFEKDHGWTFHGHEVVGSRMVPKIFGKLKLPMNEKMKFVQK
                     LVEYHLRPISLTKENITDSAIRRLLFDCGDDIDALMTLCEADITSKNKQKVKRYLENF
                     ELVRERLKEVEDKDRIRNWQPPVTGEMIMETFALPPGKLVGEIKSAIREAILDGVIAN
                     DYASAHAFMLKKAAELNLPAVK"
     gene            complement(19062..>19339)
                     /locus_tag="VGQ51_18820"
     CDS             complement(19062..>19339)
                     /locus_tag="VGQ51_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV9038701.1"
                     /translation="MNYVNDSAPMHFASAVNAPVTAVYCSTLPEFGFGPLSDDSHIVQ
                     IAQHLDCRPCGLHGYKACPLGHFNCAHKIENGQLMQTMTGGSLSQRH"
BASE COUNT         4057 a         5232 c         5418 g         4632 t
ORIGIN      
        1 tgtagatggc attatgacat gatttgcagg gctcggcgcc gacatatcct gaaggaggct
       61 gcggtcgggt ccaacgggct ccgatccccc cgatggcgag catcaatatt gccacaatat
      121 agtatggcct atttggccgc atacgatcgt ttgagattga tcttctgcaa tacccattct
      181 ccgatatggt cgccggagct aacgccggcc tcgatggctg cgcggtaatg gatgccgcca
      241 tataaccgcg agatcgccgc ctcccccgcc gcctgtctga acgagtcgaa atgccgttcc
      301 ggcaagccga attccagctc ggtatcatcg gtaaacgcgc agtgatcccc aaaccacttc
      361 gtcaggacga cagcggcgac ggtcgagatg atgctatgcc cgctggtata ttcggggaaa
      421 ggcggcgtct gaaggatggg tcgccagcct tcatcgatat aggagtcgat ataggtctcg
      481 gggcggatga cgttgctccg gtatttttca tcccagcagc taatgatggc atcgaagatc
      541 gcgatcgagg tgatcgtata ggcgccggcc gtctccatca tatccaggtg tcgctgacgc
      601 gaaactgtcg atacgatgga tatccagtgc cctccgggcg atatcttctt ggtcgaatat
      661 ccgacatgcc cttccatggt gacggcaaag gggttacaat cccagaagtt ggcaatgttt
      721 ttttgttcat ccgtcaatgc cctgacggtg cgaaagacgt cataagcctg gtgataaaaa
      781 tagctgccgg tatccttgct gaagggcggc gcctttgccg gcttgaactg cccggcggag
      841 tccagcgtca caggccgtat ccggttccaa tagggctcta ccgcggacat atatacgggc
      901 ggcgtcggta tccatttgcc cggctgacgc aggatgcgat accttggcag ccgccgggtc
      961 tcggtgtacc gatccttatc cgaccaggcg atgatcagag aggcaacggt ctgcccgtac
     1021 ttcagcgagg cctggtattc cgcggcagcg ggcttttgca tagacccgag tatagcccgg
     1081 atggaatcct gcatgacgct atcggaaaag atcatacgcc ggcctaccag caaatacgca
     1141 tagacggcgg cgaggggaaa atcgatcttt ttttcggggg ccggtatgtc gggaaaaccc
     1201 ttcacctggc cgtataatga gatataatcg tgtccatgcg ccttgacgag ggtttcgtat
     1261 gcggcgatat tactgtagag ataaatacgg ctggccacgg gcggggaaaa gatatcgtgg
     1321 accatgatat ccgtcagcgc cgcctgcgcg cgtcgcaggg gttcggcgtc atcggcgata
     1381 ggtcccggtg ccactatccg cctgacgaag gctcgcgtcc caccagcctg tgcaaccggg
     1441 cgcggctgcc cgttcacctt cagcacctgc acggcatcat tgttcttcgc taccacaagc
     1501 aaccgatccc cgacgcgatc ctttacttcc accattcccc ggacatcgcc gccgatgtac
     1561 gcgccggaga gggcattgtc cacgggctga agatccggtc cgcgaagcaa catacccagc
     1621 ccggcatcgc aacggccgag ctgcgtacgg tatgggaaaa agttcccgga tacgaggata
     1681 tccttctttc cgtcgccgtc gaaatcatcc acgacgacac catatatctt cgagaattgg
     1741 gcggccgtag gcagcggcga aaatgagaac cggccgccgc cccggttgta aaggacgccc
     1801 gaagccagct gatcgcactg taataccgtc gccttcgcca atttttccgg cggaaagata
     1861 tctgtgatcg tggcgtccgc gtaggcctta tagctgttga acttcttccg gagcgtaacg
     1921 atttgatcga gcagctcgtc gcgcgacgcc atcgggtagc tttttccccc gatataataa
     1981 caaaggatcg ggtcgagcga accgttgccg tcgaagtcgg aaacatacaa tgtcataggc
     2041 tgcgacggcg agaccttgaa ctgattgttg agaccgcaat tgccgaggat aaaatctatt
     2101 ttcccgtccc cgtcgacgtc agcaggcgta atagaggacc acatcccgtt aagatgtgtc
     2161 agacccgcgg atcccgagac atccgatagc cttcctttat cgttgcggaa aagacgcacc
     2221 ggcatccagt cgcctgcgat cagcagatcg gggtaccccg gatcgtcgag cccggtgaat
     2281 acggcggctt ttaccatccc gggaacgcgt agttcgggac atacctcatc ggtcacgtcg
     2341 gtgaaccgga tggcgccgtc tctggagtcg ttgcgcaaga gccaactgcg tgagggcaac
     2401 gggaatgacc ccgggacccc gcgtcccccg acgaacaggt cgggctttcc gtcatgattg
     2461 aaatctccgg cggcgatggc gaatttgctg ctggccatga ccgggagggc gccctctggc
     2521 gcgagagtga aattcccttt cccgtcattg atatagaggt gatcctggta gcccggacct
     2581 tcggaatatt cattgccgcc gctgacgaca tacaggtcca tgtccccgtc gccatcggca
     2641 tcgaagaaca gggcattcac ctgctcgctc acggaatcgg cgatccaggg ttgcgacggc
     2701 gcccgaacaa aatgtccgtc ccccgtttgc agaaatagct gccccgtctg accgatggcg
     2761 cctcctaaaa agatatcttc caacccatcg ccgttgacgt cccctttcgc gatggcgggg
     2821 ccttcgcgcg agagctggta gggcagcagg ggttcgtctt tgaagtcgat gaaatcgttt
     2881 tcgcgatgac ggaaggaaat gccggaagac gcggtaacgt cggtgaacag gcgatttggc
     2941 tccgcaatcc gcacaggcgc ggcgggcccg ttttcccgga catccaatgt cctatccgcc
     3001 tccagcctat ccagcaccgt cacgctgtca tcgggccatg tgatcgtgat ctttcgcgca
     3061 tccgtcccgg aaggccaccc gaaaaaaagc tccggcgcca tgctggactg aaaaccccgc
     3121 accggataca attcctcata ctggctggag ccatcgctgg tcgtcaacac cgccctggcc
     3181 ccatacgccc tcgtattcaa cccgcggccg gtcagccgca accggatata atgatctttc
     3241 ttcagctggc ttccgttatt ccggtagacc atcaccggct cgtcgttggt gcagacgacc
     3301 agatccagat cgccgtcatt atccagatcg gcatatgccg ccgcattact gacggccgga
     3361 tgcgaaagcc cccagcccac cgtcatatta gaaaaagtga gatcgccgtt attgtgaaag
     3421 atatagctgc tgagcttatt ggagggcatt ttcttcacga gatcatacgt cctgaaattg
     3481 gtgtgaccct cacgcgccgc ggccagctga gcatccggga cggtgtactt cagaaaatcg
     3541 agatcggtaa aatcgcgcag gtaaccgttg ctgatgaaca ggtccttttt tccatcgttg
     3601 tcgaaatcgg caagcagcgg cgaccagctc cagtcggtat tggatactcc ggccagctgt
     3661 ccgatctcgg aaaaccgcag atcccccttt tcgtcctcgc cttcattgag atgcagcata
     3721 ttgcgcatct gctgatgata atacccgctg tccagcagta gatgatactc atcgtattca
     3781 tcgggacctt tcagcaactt ttgccggtga ttgtcctcgg gcagcatatc gagagtcatt
     3841 acatcagggc ggccgtcgtt gttatagtcg gcgatatcga gtcccatgga atatttggag
     3901 atgtgggtaa aggacttctc gagcgattcg gtaaaagtgc cgtcatgatt gttgatatag
     3961 aaacagtccc gttccgtata atcgctggtg gtatagatgt ccggccagcc gtcgccattg
     4021 acgtcgctga ctacgacgcc gagtccgaaa ttcaaaggat tattaataat tccggcttgc
     4081 agcgtgacat cggtaaagtg gatgtgccct tccttatccc tgtcctggcg gaaaagccgg
     4141 ttgccgaaat gcatgtcggg agtggcgcgc gtctgacgtg tattcagata agggttatac
     4201 gtatggttgg aatggttgag caggaacatg tccagatcgc cgtccttgtc ataatcgaaa
     4261 aagacggctt gcgtcgattg ggtacccggc gcgtcaagac cgtaggcggc ggccatatcc
     4321 ttatagacgg ggacaccgtt ctttattccc tggcagataa aaagctcgtt gctgaggcgg
     4381 gtcgaatcat cgtatttgcc ggcatgacag acatagatat ccagcagacc gtcgccattg
     4441 acgtcggcga tgctgacgcc cgtgctccag gtcccgttgc cggaaaggtg tgcctgcctg
     4501 gtcacatcct cgaaccggaa attccctttg ttgagaaaca atttatcggg accggtattg
     4561 ccggaaaaaa aaatgtcgtc gagtccgtcg ccattgagat cggcgatccc gatgcccgcg
     4621 ccgttgtaga ggtactcgta gctgaaaaca ttgagcgaat cgtcttcccg gatattgttt
     4681 gaaaacgata tgccggtctg tttttcgccc agcaacgtga acagtgtggt gtccggtctt
     4741 tgagcagcca gacgcaaggc gcccgaacaa accagtatgg cgagtagttc tttcatggca
     4801 gatcgatatt ttcgtctcct tccttataag gcaaaaaacc catcatgagg gtcatcattt
     4861 catctgtggt cttcatatcg cccatcgaga aaacattttt gggcggattg ttcggattga
     4921 agggattgtc tgtagtgttg tcataggtgc cttcgatgtg gatgaccgat ccctggacga
     4981 ccttgatcgg atggcggaac cagtagatct cctgccagcg gaagtcccaa ttgggaatat
     5041 gcaccagccg gatcgtatcg cccgccgggg tggtgatata ggccttgtac tctttcccca
     5101 gcagatgcat atgcggccag acgtagagta aggactggtc ctggctcgga ttggtgacct
     5161 cgagatgaaa ggtctctact ttgccggcct tgatatagaa gatcggctcg atctggttct
     5221 gaccgatgcc ccccgacccg aagctcacga ctttcatgac acgcgtgacc ggcgtcttct
     5281 tgaaaaagat attgacgccg caaatgctgg tctcatcggt ggctgaagga gcatagtgga
     5341 cggtcagcaa aataaccccg cgccggggca tcacccatcc gaattccttc gggaaatgtt
     5401 catagctggc gccggggatc cagccgccat agtaggtgat ggtcttacgg tagggttggt
     5461 attgatcgaa tttccggcgg tcgtcctcgg tcagattgag catcgcgtcg gtatggtaga
     5521 tatcgatatt cgtgtcgggc acggagtgga tggcgtagtt cgcgtggtgg atcaactttt
     5581 tgttgctgca gaagaactcg atggcttcga cgttggccga gtccttcagc tcgaagggta
     5641 tcttgaaaat gatatagcgt tcgatgttgt cgccggttag gcgaaaactg tcttttgctt
     5701 tcaggaccag gtcgggcttg cgaaaatagc tggtgccggg gatggaggct tccttcagcc
     5761 cggccgtgtc ggggccgtcg ccgcgggggg ctttgttgtc gatccattgc agcagcagtg
     5821 ttttttcttt aacggtgagc gaccggtcgt tggcaaagtg tacgtagttg ttgtcggctt
     5881 tccatggcgg catgtacccc ttttgcacaa catcccggat aaaggatacc cttttggcta
     5941 cgtcctcata ggtgataagc gaaaagggac cggtctgccc gggtttgtgg caggtcgcac
     6001 atttgctacg gatgatgggg gctacgtcca taaaagtcgg gtgcgaagac tgggcgaagg
     6061 acggtttggg tccggtaagg gtcaggaaga aggcgatatt cagaaaaatt cgtttcatgt
     6121 cagtaatcgt tgatcaggca ccctacaggt ttgttatagg gaacgcggac aggttggtgc
     6181 gccagcgact gatcgatggc gtcccgcagg taaaaaacgg ttggccgggc gctctgcatg
     6241 cccaggtcct ttatccagtt gtcgatagag ccgtgataga ttactttctt gtccgggccg
     6301 aggagaaaca cttcgggcgt gacggtcgct tttaaggacg tggacatggt cttggccgaa
     6361 tcgatatata cgggaaaggc gatatggtat ttttgcgtga atgcgttgac atcgccggcg
     6421 gaataagctt ttcccgggac gacgcccgtg aattgaacct tgtcggcgta ttttttgaat
     6481 agcgggttca gtgggagcga atagttctgg caaagagggc cttctggtga aagaaagatg
     6541 acgaccctca ggggtgcggt gcgcggttgg gcccatgctc cggagaacac cagtacacac
     6601 gatacaaata acggcaaaag caatttcttc atgaacctaa aattaaaaaa ctccgggccg
     6661 ataacagccc ggagttcgaa ctattttgcg gttggctatg gcatgtccca ccaaacacga
     6721 ccactgaagt cgttgggtcc ggtgccccag ttgccgtcgg aggtcatgtt cttgaaggcg
     6781 gcctgctggt tctcgtagtt acggtccgtc gggagcagaa cggtgatcgc cgcgcgccgt
     6841 ggcagagtca gcactgtgcc gctggccgtg ggttgttccc atttgatcgt agaggttgtg
     6901 ttcgggaagc ccgtccgttt ccaccacgcc caggcctcgc tgggttgacg gaagaagtcg
     6961 agataggcct ggcaaacgat ctggtccagg cccgttgccg gaacgtatgc gaccttcgtc
     7021 aggtaagtcg tgatgtcgga agcagtgacc ttggtatacc ctgcgatccc ggccgcctgt
     7081 gcacgctggt cgtagtaggt gatcgaagcc gtcacgcctt tgttgtacca ggtagcggca
     7141 tcgtcgccgg ttacccctct gttaccaagc tctgccctga taaaacagta ctcggcatag
     7201 gtcatcagcg ggaagaatgc gacaccggtg cccgaaccgt tgccgtcccc ttcgtcatag
     7261 ttgggcgcaa aaagcctgtg ctgcagctgc gagaaagcgc tgtcggaatg caggaacaac
     7321 tgggcattcg gagcaacgtc gtcggggctg gtaggtgcgc cgacaaaagt tcctgtccgg
     7381 agggcaggac gatagaatat tttttcccgc ggatcacccg taaggttcat gaagtcgacg
     7441 acgggtttac ccgcgcagaa ccctaccggg ttgtagttgc ccgtgccatt ggcgaaggcg
     7501 ggacccgcat acagtgccca tgtgtcgtct gtcgaagcca tctgcgacgc atcggccagg
     7561 acttccgtca cgatcgtctt catcctggtc gggtcacgct tcatcaggcg catcgccatt
     7621 ttcagacgga cggcgttgcc gatcttcgcc cattctgccg cttctaatgc ggcattacca
     7681 cttatacttg taggaaagaa ggggtctttg gcgcccatcg ggacctgtgt ggcagtctga
     7741 gccgtctcca gtacttttac tgccgccttt atctgtgtgt ccagcgtatc gaacagcatc
     7801 tgctggcggt cgtacaccgg agtgagcgtt ccgccatagc gagcctggaa ggcttgcgag
     7861 taagggatat cgccattgat atccgataca tagaatgcgt aataggcttt aaagatagcg
     7921 ccaatgctca gctgataggc gcgctttgcc tggtcgcccg gtttcatctg ggagacgagg
     7981 taggtcatgt ccgacagata ggtaccgacc ctgccataaa agatcttgct ataccgttca
     8041 ttaaaattgc cggtctccct tgcgaaaccg ggggtattgc cggccggact ggtcgtatac
     8101 tgtaaccagt tattctccgc gcggtataca tcataaaaat actcgaaatc gtcctggcaa
     8161 cccgctgccg ccgcgaggaa ctggtcggcg ggatccaccg catacagcgt tgtggggttc
     8221 aggttggtct cgacgaacgc ggacttttta caggccccca tcgccagaca ggctattgaa
     8281 agcgatacaa tatatttttt cttgttcata agttcaattt tcgtgttgtt agactttaga
     8341 aaccagcttt gacactgaac ccaaggctgc gggtgtacgg cagaccaccg tattcggcga
     8401 accctgctga attattgtta tagaaacctt cggggttgat gccatccttc gccgtcttgt
     8461 acagatagcc aaggttacgt ccggtgacgg ctacccgcag cgagctgaat ttcacctttg
     8521 tgtagatgct agcaggcagg tcataagcaa gcgtcacttc cctgaccgcg atccaggagt
     8581 tgtcgaaagt cgaatattca cggatgccgc tggaccactg gctcaggttg ccataataag
     8641 cataggcggg gacgggtgtc accaggtttg cctttacggc gtctgcatag ctcataccgc
     8701 tgacgtcgac gcccttgatc acgataccgt cgttgaatac accgtcgggg atgatgccgt
     8761 cgttgtgtga accgttcgcg tccgtataag cgagaccgcc agatgcggcg tcacggccat
     8821 gaagcgaatt cttgaaggag ccgcctgcgg agccatactg gtggctggca gatgccatca
     8881 gaccgccgta tttggcgtcg atctgaacgc tcagattgaa gctcttgtat cggaaggact
     8941 gtgtcgtgct gcccagccag ttctccatga tcgtgcccag ttccttggtg ctgccgtcat
     9001 agtcctggct acgcaggtaa gaatagtatc cgcctgtagt tccataggga gcgagaccca
     9061 acactttctt gccggtagcg gcgctgttct ttccgttata gatggcatag ccataacccg
     9121 taacgacggt accatagggc ttgccggcct ctgccaccgc ctgctcgtcc gcgccgaatg
     9181 ccagttccag ctggtaggat ttgacgccgg gcgcgaggct gatgacctgg ttcctgttgc
     9241 gggtgaaatt gatggtggaa ttccaggtaa aattccttaa ctgcaccggg gtagctgtca
     9301 tcaggatctc gacaccctcg ttctggatat tacccgcgtt gatcgcctgc gagctcgcac
     9361 cgctctcaac cggggtgttg aggttcagta tctggtcgaa ggaattcttc ttgtaccagg
     9421 ccacatcgaa gcccaggcgg ttgttgaaga agcggatatc ggcgcccaat tccagttcac
     9481 gggcgcgttc tgccaccagg ttggggttgc caagattcga gccctggaac gtaaacagct
     9541 gctgggtccc attgccggag ttgttgaaag tgccgttcgg cgagtagaaa ccagccgtgt
     9601 tcgtataata tgcctgcggg taggagcctg accaacccag actggctctc agcttaccga
     9661 aggacagaat gctatttgaa ttcttgaaga aaggcagctc cgtgaacgtc caggccagac
     9721 caaccgccgg ataggtcgcg taatctttgc cgtggccatc cttataggtg agtgaggaga
     9781 cccagtcgtt ccgcagggtc gcattcgccg tcagcatatt tttccaggta aggtcgccca
     9841 tcacatagac ggcatcgtta cggtagctcg aatatggtgc gtccgtgttg ttaacctgga
     9901 tcgatgccgg gctgacggaa ttggtgatcg tatagagtcc cggggccagg agaccgccgt
     9961 tcgtgttcag ctggatccgc tgtccgccga gcctgcggta ggtttcaccc cctgcggagg
    10021 cattcagcga gaaatcctcg ccgagttcct tggtatagtt caacaatccc tgtacgcgga
    10081 tattgttgta gctgcccttt ataagcgtat agctgccgcc ggaaaatcct tttccgctgc
    10141 cgttgacgtc ttcttccgaa tagaaattgt aggtattgac attgctccgg acgagggcgc
    10201 tcagcccggg tgcgagcttg atggtcaggt ccagatccgc cagcaggttg ttctcatgct
    10261 gggtgctgtt gtcctggtaa tagttccaca tcgtatgcgc gagataatac gggtcaccgc
    10321 tggggcctgt cttctgaccg ccgttgacgg tgtcgatata gttgtgctgc caccagtcga
    10381 tcggcgcatt acgcggcata aaataggtga acgcgaacag cgggctgaag ttaccaccct
    10441 gccagatggg gttcttgatc gtggagatcg tatagctcac gctggcatcg agggtgatga
    10501 ggctgccgat cttatgggtt gcgcgcagcg agaaagcgtc tctctgaagc ttgttgttcg
    10561 gcatgatgct attgttttgc agatcggata ctgaaaaacg ataggtgctg ctttcgttgc
    10621 cgccttcgat ggcgaggttc gtattgatgt atttgccggt ctggaagaag tcgagcggct
    10681 tgttggccac ccagggtacc atgcgaccgt cgatgtcctt gaccatatgg ccgtcgaatt
    10741 tcggaccgaa agactggttg gcgtccgggg tattgtagat actggcgttc agcgggtcga
    10801 cgatgtcgtt gccctgggcg tccttatcga aagtgggctt gatgccgccg ccataggtat
    10861 tctgatagtc gggaagcttg taggcgtgat cgaaagattc ggtatgcgta aaggttatcc
    10921 cgaggtcttt gcgggcccgt ccctttttgg tggtgatcag gaggacaccg ttgagacccc
    10981 tgctgccata cagagccgtc gccgcagagc ctttcaatac atcgatgctt tcgtagtcat
    11041 cggggttgag atccttgatg atattgccga agtcgcggtt ctggccccag gagtcggccc
    11101 ccgtagtgcc tggctcgatg agtatcccgt cgatgacgac cagcggctgg gtattgctgc
    11161 tcagactggc gttaccgcgg atgcggatct tcgcgctgga ggaaggaccg ccggcgccct
    11221 ggccgacgaa cacccctgcc accttgccct gaagggcgtc gacgaagttg gcgttattcg
    11281 cctgggtgat ctcgtcgccc ttgatctcgg tcacggagta tggaaccttt ctcaggtttt
    11341 tccgctgacc gaaacccgtc acgactactt cgttgagact cttcgggtca ttgtgcatcg
    11401 ctaaggtaat ggtgccgccg gtcacttcga tctgctgcga caggaacccg atacccgaaa
    11461 acacaaggtg ctcacccctt tgtgccccga tcttaaaatt gccgagggcg tcagttgagg
    11521 tgcccgattt ggtgcctttg acagtaacac taattccgga gaggggattt ccaacactat
    11581 ccctgacgtt gccggatact gaaatccggg tctggccaag tagtgctgcg ctccacatgc
    11641 aggttagcat gaacaggagc aaatgagcct ttaactttct catttacgtt gtagttttga
    11701 tttagaaaat gctttttcaa ttatctttct tatggccgtt caatcctttt tccgatgctt
    11761 ttctcagcgc gccatgactg cactcgctcc tttctttatc gatcctttta cataattttc
    11821 cattaccagc gcggctgccc cgatgagttc cgcgtccaac cccagttcag agacctcgat
    11881 ggtcgtattg gccgaaagcc tgggtataca gtgctcgttc agcgcctgtt gtatcgcggt
    11941 ctgccagatc tttcccgccg cggcccctct cccgccgagt acgatggtct cggggttgag
    12001 cagatggata aggatcgcca caccccgtcc gatattgtaa cccgactcgg acagcagctc
    12061 gaccgcgaac ttgtctccct tgccagcggc ggatatcacc gccagcgagg cctgatccgg
    12121 gttgtccgcg agctgctgtt gagtgagcat ggtcaccctc ccaccggcaa tgccttcctt
    12181 cgccttctcg accatgacca gcagcgatgc ttcggtttcg agacagccgc tctttccgca
    12241 gctgcacagt ttgttgttgg agaacagcgg caggtggcta aactcacctg cgaaaccatt
    12301 cccaccccgg aacaactcac cgttgaggat gagtccgaga ccgatgcccc agctcaggtt
    12361 gatgaccatg gcgctcttct tatgatatgc cgcgccaaaa ctcaattctg caagggcgat
    12421 caggctggaa tcgttttcga tgtaagtcct gaccccgacc ctttccatta tatattgtga
    12481 aacacttctc tcgcccgcgc ccatgaaaga gtaattgatc ccgttccgtg agtcgacgaa
    12541 acccggcatg ccgatgccga tgcccgccag cctgtctttt ttgatgcctg accggtggat
    12601 gtgctcgtcg atcttttctg ccagcgtctc cagcgccctg ggattctttg ccaggtgcag
    12661 ctcgaatttc tccgctgcag tcaccggctg ccgctgcata tccatgatga cgatacgggt
    12721 gaagagctga tccatggcca cggcaagcgt gtacatcctg tccggtcgca aagaatacat
    12781 ctgcggcctc ctacccccgg tcgaaggagc gtaaccggtc tccatgatat cgttgtccct
    12841 gatcaattcg ttcaccattt tagtcgtcaa gggtaaactc gtttttatac gcgcagtgag
    12901 atcggaacag gataaggcat tcgcgaaata tagctgtttg ataatccttc ctttataaag
    12961 caagttttta tcgcgcataa agctgtccgg gatggctctt aatttaattt aacatgaagt
    13021 taatgaactt ttataaaaaa attaaaaacc tgttaaaatt tttataatag taagacaagt
    13081 tagacaaaac gatgatctat tagccattgg ctaagaataa attggcggta attggtggta
    13141 ggaaaatgtt cagctctttt tatccgtaag ccagtcgcgg aaccaatagc cggagctttt
    13201 gatggtccta agctgtgtcg cgaaatcgac atggatgagg ccgaaacggg cgcgatagcc
    13261 ttctgcccat tcgaaattgt ccatcagcgt ccaggcgaag aagcccttga tattgactcc
    13321 ctccttccgg gcacgtagca aggcgagcag gtattgctga taatagtcta tacgggcttt
    13381 gtcgtcgacc ataccaccga cgacctcatc cttgaaagcg gcgccgctct cagtgacaat
    13441 gatctctttc acgccgccat acagccaaaa tcttttgaga atgcgataga aactgtcggg
    13501 attgatctcc catcccatgg cggtataagg cacgccgcgg ctggaggcct tgacctccga
    13561 ggcctgcacg atggggatca gcgggttgta cctgacgacg atagggaaat aattctggat
    13621 gccgatgaaa tcgaagttga actgcatccg ctcggtatat ttccagctgc gattgtgcag
    13681 ctcgagtctt tcgaggaatt tgaagtcttc acggggatag ccgcgcccga gggccggctc
    13741 gataaagagc cggttcatga gaatgtccag gcgtcgcgca gcctggatgt cttcgggaga
    13801 gttggtatag ggcaccacct ccgaacagga aaagctggtg ccgatgtgcg ctccgggaac
    13861 cagttgccgg atgatcctcc ctccttccgc ctgtgccagc gcggcatgat ggatggccgg
    13921 caggaaattg gaaagtccta tcctgcccgg cgcatgacgt cccagcatat atcccaggct
    13981 ggtgaaaccg aacggctcgt tgagaacgat ccagtccttg actgcaggtc cgaattcggt
    14041 ggcgcagagt gtggcccaac gactgaacca tcgcagcatg agatgactgg tccaacctcc
    14101 ttctttttcg agtgtaacgg gaaggtccca gtgatacagc gtgacataag gcgtcagccc
    14161 gagcttgtga cattcttcga tgaccttgtg ataaaaaagg acgccttctt tatttacgcg
    14221 gccgacgccc tcgggcagga cgcgggccca cgacaatgag aaccgaaaaa ctgaaaaacc
    14281 cagggctttg gccagcaaga gatcgtcctt ataacgatga taaaaatcac aggccacggt
    14341 gggacggccg ccgcctttga tctttctctg acgccgggag aaggtgtccc agatcgaagg
    14401 cccctttcca tagctgttcc atgcgccctc gttctgtact gcggccatgg ccacgcccca
    14461 taaaaaatca tagccgaatt ccctgacgtt cagtttgtac agttggtcca attttaatct
    14521 ttagtgactt tttgcgagtc gatcagggta ttgtagtagg tcactacctg cgcatattca
    14581 tcaggggtac gccctttcag atggttgtca cggacgaagg cggctaccgc ctggggcttg
    14641 tcgccgagta gcgcgggcag atcggaccag ctgcggatac gaaggaattt gccatttatt
    14701 tgcagataaa agaagtggac atcggtgatc gtccgctcta cggtcgagcc gccatacgga
    14761 gtattgtcgg ccatcttttt gcggatctcg tggaggaggc tgaccttatc attgacgagc
    14821 acctgcagcc aggcgccgtc gagggctttg tcgatgccgc gccagggagt acccggtacg
    14881 aaggtaatag tgcccgccgg actgctgagt gtcaccgcac ggataggcgt ggtcgctatc
    14941 atctcctggc catcggcgtt cttataatgg atctcttttt gaagcagatc gagtttaagc
    15001 cggaggtgtt cgatcacctt tccgccggtc atcagcaggg cgccatccgc ccattcatcg
    15061 ctgaaccatg gcgagccttc ggtcagcttt acataggtaa caggcgtgat cacctggcct
    15121 ccgaccaggc cgtttatcat ctgctgtccg actacatcgg catcgctgtg ggtgatatcg
    15181 acgaccttct gtgcgcgggc gaagacgctg ccgataagga cgactgctat ggttaagatg
    15241 atcttcattt aatgagcgta ttgtaatagg cgactacctg cgcatattca tcgggcgtgc
    15301 gtcctttcag gtgatggtcg cgggtgaact tcgtcaacga ttccttcttg tccccgaaca
    15361 tcgcgggcag atcggaccag ctgcgtaccg gaaggagttg tccgttcatc cgcgcataat
    15421 aaacatcgtc atcggagatg gtccgctcgg cggcggtacc gccaaaagtc ctctgctcga
    15481 tctgcttctt ccggatctcg tgcaaaaggc ttaccgtgtc attgaccagc acctgcaccc
    15541 aggctccgtc cagggccctt tcgaccgagc gccagggcgt acccgaaata aagatcaggg
    15601 gccccgtgga accggcaagc ttaaccgcgc ggacaggcgt ggtcaatatc atttcctggc
    15661 tatcggcatt tatataatgg atctcctttt gaagcaggtc gagtttaagc cggagatgtt
    15721 cgatcacctt tccgccgggc agcagcaagg cgccatccga ccattcatcg cggaaaaaag
    15781 gagtaccttc caccactttc gcatattcat tgggtttgga tgcctggcca ttgatcaggc
    15841 tgtttaccac gtcctgcgca ataacatctc catcccggaa ggtgtataag ccgagatcct
    15901 gcgccccggt ggagaatccc ccgataagta ggaccgcggc tgtcaacagg agttttgcag
    15961 tcatagcttg aatgtacaaa aaaaaccgat gctcagagca aggtttccag cgccgtgccg
    16021 ggatctgtcc agcggaccga gagatcgcga tgaaaccagg tcatctgccg tttggcatac
    16081 tgacgcgtgg acgtcttgat ggccgctatc gcgctttcga gcgatacctt cccgtccatc
    16141 cattcgaaca gctcgcgata acccaccgtc tgcaacgcat tgtgcgaccg gtaagcgacg
    16201 agactctcca cctctttggt caggcccgcc ctgatcatgg cgtcgacgcg ttggtcgatg
    16261 cgccggtgta actcgccctt tggcaactgg agaccgatgg tccggatccc gaacggacgc
    16321 tgcggccgct gtttggtcct gaacgaaagg atggactgcc cggtggacat cctcacctcc
    16381 agggcgcgca tcagccgttg agggttgagt atctcgccgg cagaatagaa atcggggtca
    16441 tattttcgga cctcttcctg caaccaatcg aggccctttt cttcataatg atgccggata
    16501 tgggttcgaa ttccctgatc caccggcgga atagcgtcga gtccgtcggt gaacgcccgg
    16561 atatagagcc ccgtcccacc caccatgacg gcatagggag atttgcgaaa gatctccgac
    16621 gtccactgca gcgcgagctc ttcgaaagtc gcggcgttga cctcttcggt gatggagtga
    16681 ctgtttatga aataatgcgg tacgagcctg agctcttcgg gggtcggttt ggcgacaccg
    16741 atatccagtt ctttataaca ctggcgtgaa tccgcagaga tgatgcgggt gcccagatga
    16801 cgggcgagac gaatggctgc cgccgttttg ccgacggccg tcggcccgac gatgattatt
    16861 acggtaggct gctgcaccga tgttggtggt taaagggtgt cttcttgcgc gccggcttcc
    16921 ccttcccctt ccccttcccc ttcctcttcg gactctccct cttccgattc tccttctacg
    16981 ccgaagcctt cggtgccctt ggtgaggtcg tatttctctt ccatctccgt gagcttgtcg
    17041 ccgacgaggc ctttagtgcc gtattgggac ggagcgatac cttcggtctt gacgaccaca
    17101 gggtaatcca ccttgctgcc ttcttctttg gagacattga tcaactcgac gaggaaggac
    17161 cagttcttcg cgaaatcgta gacgtagatg aatttctgat tggggtcgcg gatctccgag
    17221 ccgatggtcg tcgcctccat cagcaggggt tccttattgg gatagttttt ttcgtacaat
    17281 tccagcgaga tctggcgacc gcgctgccag tggtcattac tgcggtaaaa ggtggcctgg
    17341 tgcttgctat cgaattcata ggatttcagg atcacctggt gcaatagcag gaaagattgc
    17401 gtgtgccgta tcgccacatc cctgtaaatg ctgtcatcct cctccaaata gacccgaaat
    17461 ttcagtatcg ccatggctac aagtcgattt tgagttcaca agatacataa atccaacaat
    17521 tatgccaccc ctgccgcaga aggctggatc aacgcctttg gacaggggac ggacggctta
    17581 ttttacagcc gggaggttta gctccgccgc ctttttcagc atgaatgcat gggccgatgc
    17641 gtagtcgttg gctatgacgc cgtcgagtat ggcttcccgt atcgcgctct tgatctcgcc
    17701 gacgagcttc cccggcggca gggcgaaggt ctccatgatc atctctcccg taaccggagg
    17761 ctgccagttg cggatacggt ccttatcctc cacctctttc aaccgctcgc ggacgagttc
    17821 gaaattctcg agatatcttt ttaccttttg cttgttcttc gaagtgatat cggcctcgca
    17881 aagggtcatc agcgcgtcga tatcgtcccc gcaatcgaac agaagccgtc ggatggccga
    17941 atccgttata ttctccttcg tcaggctgat gggccggaga tgatactcca ccagcttctg
    18001 aacgaacttc attttctcgt tcatcggaag cttgagtttt ccgaagatct ttgggaccat
    18061 gcggctgccg accacctcat gaccatggaa ggtccagcca tggtccttct cgaacttctt
    18121 tgtcgccggc ttgccgatgt cgtgcaggat ggcggcccat cgcagccaga ggtcgtccgt
    18181 gttccggctg atgttgtcga gcacctgaag ggtatggtag aaattgtcct tatgccccat
    18241 tccgtcgatg tactcagcgc ccgacagatc gaccatttgc ggaaatatcg tcttcagcag
    18301 accgctgcgg tacagcaggt cgaatcctat tgaaggtttt gccgccatga cgatcttatt
    18361 cagctcgtcc gttatccttt cctgggaaac gatctttatc cgggggcagt cgcggacgat
    18421 ggccttccag gtgtcgtcat cgatggcaaa acccaattga gttgcaaaac gtatgccccg
    18481 cagcatgcgc agcgggtcgt cgctgaaggt cttcaggggt tcggtcggag tgcggatcgt
    18541 ctgctttttc agatcgtcta tgccgccgaa aggatcgatg agattgccgt agtcggcttt
    18601 gttaaggctg atggccatgg cgtttatagt aaaatcgcgg cgcagctggt catcttccag
    18661 cgtgccaggt tcgacgtcag gtttgcgtgt atgatcgcgg tagctttccc ggcgcgcgcc
    18721 gacgaactcg atctcccagt cgctccattt gatctgggca gtgccgaaat tcttgaaata
    18781 agatacgttc ggccggggat gcagggtatc ggctactttg tgggcgagcg agatgccgtc
    18841 accaacgcac acgatgtcgg catctttggt cggacggccg atcagcttgt cacggacgaa
    18901 accgccgatc aggtacgcgg gggtacccat cgcttcggcc gcccgggcta tcttttcgaa
    18961 gacctccagt tccttatctg tgcattgtat gtccaccgcg cgaaattata gattatgcgg
    19021 aagattcgct tccggcaatt tggcgaatct tccgcacaaa gctaatgtct ttgtgacagg
    19081 ctaccgcctg tcatggtttg cattagctgc ccgttctcta ttttgtgggc gcaattaaaa
    19141 tgccccagcg ggcaggcctt gtaaccatga agtccgcagg gcctgcagtc caggtgttgc
    19201 gctatttgga cgatatggct atcatcggat agcgggccga atccaaattc cggcagcgtt
    19261 gagcaataga cggcagtgac gggggcgttg accgccgagg cgaaatgcat cggtgcggag
    19321 tcgttcacat aattcatta
//
LOCUS       DASYSV010000603         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2429709, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000603 DASYSV010000000
VERSION     DASYSV010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2429709"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VGQ51_18825"
     CDS             <1..190
                     /locus_tag="VGQ51_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV9038702.1"
                     /translation="QKRRFVPDFFFPVLGFLVCFVLWISLPTAAKVVGGIWFFIGLVY
                     LIIKTQGLKKKPVILDFK"
     gene            196..1644
                     /locus_tag="VGQ51_18830"
     CDS             196..1644
                     /locus_tag="VGQ51_18830"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HEV9038703.1"
                     /translation="MKKLLLLVLSFLGSVAFAFAQGPGGILKGNMTQANITPPVGCRL
                     AGHFYEIFSTGVHDSLWAKAMVLQQGKEKFAFVFCDLIGLTTHVSANARLQASAKTGI
                     PVKNIMITAAHSHTGPLFYGFQHTYFHRRALAAGKGQDPHERLDYSRFLVRKIVAAIV
                     KANGAIHPVQLVAGIGEQNGLSFNRRYYMKHGPVLFNPGPLNPDIAGPAGPIDPAVGI
                     LLLRDGRSQKYEGGLTVFAMHADCIGGTEMSADYPYFLERTLKGRWGKGFISAFSLGP
                     SGDINDVDVKKDQPIYSAANTERIGQTLGKTVINTVPGLPVISRPALAMLSTKILLPL
                     QVPTKEQIDSARVLINSLYEVKKTGRYLANAGGESGDFLKRVEMSKYLALADRKKAVQ
                     VEVQVFRIDDETALVGLPGELFVELGLAIKKRSPFKNTIVMTVCNDKTSYIPTQKAFR
                     EGSYEVTNAIVKSGDGEMLVTTALRLLAEIKR"
     gene            complement(1683..2552)
                     /locus_tag="VGQ51_18835"
     CDS             complement(1683..2552)
                     /locus_tag="VGQ51_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV9038704.1"
                     /translation="MKAVEYRLPQELDKSFIVFEEKGAFFPCPWHYHPEYELVLVIKS
                     TGRRMVGDHIGYFQEGDLVFMGPQLPHVWVNDEQYIQSQSKSAASAIVIQFVEKFLGE
                     YFFNIPEIEAFKKKLKLLKRGIAINGKAREKISGLMRGMLLQNGLQRLAALITIFDIL
                     SGCTEFELLASPNYEKVVRAGNSPDRFSTITEYIMNNFTKDITLPEIAGVVNMGVTAF
                     CHFFKQHHKVTFVEYLNAVKVGYACKLLGDKDESIVGIAYHSGYNTLANFNRQFRKLK
                     NMTPSEYRKTLNI"
BASE COUNT          798 a          773 c          859 g          848 t
ORIGIN      
        1 gcagaagcga aggtttgtgc ccgacttctt tttcccggta cttggattcc tggtgtgctt
       61 tgtactttgg atcagtctgc ccaccgccgc aaaggttgtc gggggcatct ggttttttat
      121 cggattggtc tatttgatca taaagacgca gggtttgaag aaaaagccgg tgatcctgga
      181 ttttaaatag caaaaatgaa aaagttactt ctattggtct tgtccttttt ggggtccgtt
      241 gcctttgcct ttgcgcaggg tccgggcggc atcctgaaag gtaacatgac ccaggcgaat
      301 atcactcccc cggttggctg tcggctggca ggccattttt atgagatctt ttccacggga
      361 gtacatgact cgttgtgggc aaaggctatg gtactgcaac aggggaagga gaaatttgcg
      421 tttgtctttt gcgatttgat cggtctgacc acgcacgttt cggccaatgc ccggctgcag
      481 gccagtgcaa agaccggcat cccggtgaag aacatcatga tcacggcggc gcatagtcat
      541 accggtccgc tattctatgg gtttcagcat acctattttc ataggcgggc tctggccgcg
      601 ggtaaggggc aggacccgca tgagaggctc gattatagcc ggttccttgt tcgtaagatc
      661 gtggcggcga tcgtaaaagc caatggagcg atccatcctg tgcaactcgt agcgggcatc
      721 ggtgaacaaa acgggctttc cttcaacaga cgctattata tgaaacatgg gcctgtattg
      781 tttaacccgg ggcctttgaa tcccgatatt gccgggcctg ccgggcccat agatccggcg
      841 gtagggatat tgctgctgcg ggatgggcgg tctcaaaaat acgagggtgg gttaacggtt
      901 tttgccatgc atgccgactg tatcggcggg acggagatga gtgctgacta tccgtatttt
      961 ttggagcgga cgctaaaagg aagatgggga aagggattta tttccgcctt ttctcttggc
     1021 ccatcgggtg acatcaatga cgtcgatgta aaaaaagacc agcccatcta tagcgccgcc
     1081 aacacagaac ggatcgggca gacccttggg aagaccgtga taaacaccgt acccgggctc
     1141 cccgtcatca gccggcctgc tttagccatg ctgtccacga agatcctgct gcctttacag
     1201 gtgccgacaa aagaacagat cgattccgca agggtgctta tcaatagcct gtacgaggtc
     1261 aaaaagacag gacgatacct ggcgaatgcc ggcggcgaat cgggcgattt tttaaagcgt
     1321 gtggaaatga gcaagtatct tgccttggcg gatcgtaaaa aggcagtaca ggtggaagtg
     1381 caggtctttc ggatcgacga cgaaacggcc cttgtaggat tgcccggcga attatttgtg
     1441 gagttgggtc ttgccattaa aaagaggtct ccctttaaaa acaccattgt catgacggtg
     1501 tgcaatgaca agaccagcta tatcccgacg cagaaggcgt tcagggaagg aagttatgag
     1561 gtgacaaatg cgatagtcaa atccggtgat ggggagatgc tggtgacgac cgcgctcagg
     1621 ttgctggcgg agataaagcg atgaggtgtc gcgcgaatat tcgccgcccc ggtattcgcg
     1681 ggtcaaatat tcagcgtctt acgatattcg ctgggggtca tattcttaag cttcctgaat
     1741 tgccggttaa aattggccag ggtattgtag ccgctatggt aggcgatgcc gacgatactc
     1801 tcgtccttgt ctccgagcag tttacaggca taccctacct tcaccgcatt gaggtactcc
     1861 acaaaagtga ctttgtgatg ttgtttgaag aagtggcaaa aagcggtcac gcccatattt
     1921 accacccccg ctatctccgg aagggtaatg tccttcgtaa aattgttcat gatatattca
     1981 gtgatggtgc tgaagcggtc gggagagttc ccggcccgga cgaccttttc atagtttggg
     2041 ctcgctaata attcaaactc ggtgcagccg gaaaggatgt caaagatggt aataagggcc
     2101 gcgagtcgtt gtagtccatt ctgaagcaac attccccgca tcagcccgct tattttttcc
     2161 cttgccttcc cattgatcgc gatgccccgt ttcaaaagct ttagcttttt tttgaatgct
     2221 tctatttccg ggatattaaa aaagtattcg ccgaggaatt tctctacaaa ctggataacg
     2281 atcgcgctgg ctgccgattt cgattggctt tggatgtatt gttcatcatt tacccacaca
     2341 tgcggcaatt ggggtcccat aaagaccagg tcgccttcct gaaaatagcc gatgtgatca
     2401 cccaccatcc gccgacccgt gctcttaatg accaggacca gctcatactc cgggtgataa
     2461 tgccaggggc aggggaagaa agcccctttt tcttcgaaga caataaagga tttgtcaagc
     2521 tcctgaggca agcggtattc gacggccttc attataaaat ttgttatgta agatacagat
     2581 ttgttagaga atagcccaag gtgctctaac cggtttaatg ccatggcagg ttagacggca
     2641 gtaacgtatt atctccacct gtttgcttcg ggataatcgc gcttttgcga ggacattcgc
     2701 atcgctacca gtttcccgtc cgtcaattca ttcatcatat tcaaaatcgg aatgtcagcg
     2761 atctccttag tcacagggca ccgctcttta ccccggtaca caatgaagcc gagagcggag
     2821 tgcagcaagc aaaaaatagg ctatccccct acgggatagc ctataaaaaa ttgaaaatta
     2881 agatcgtata ccgcgtaggg gattgatggt taattattca tgaattgtta attgcgccca
     2941 ttgatcaagc tcactaccac cttcgtcatc atctcctttt cgtcgggctt actttcagcg
     3001 atcatgagtg tcaaggcgac caatgcattg tccgcgagct ttcgagtgcc atctacatga
     3061 tacaggatgc cattcttctc caggaaccaa acgaaaagga aggccgctat ccgcttgtta
     3121 ccgtccgaga aggaatgata gagatccgtt ccgccaaagg tctggtaaat agccgccagg
     3181 gatccctgag gtggctcgaa ttacgcggac acgaaattct cttgggttaa acaataataa
     3241 tacttttttt cataggacat cgggctttga tagttcag
//
LOCUS       DASYSV010000604         4359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1654958, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000604 DASYSV010000000
VERSION     DASYSV010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654958"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VGQ51_18840"
     CDS             <1..939
                     /locus_tag="VGQ51_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV9038705.1"
                     /translation="NVKFIIEGEEEIGSPNLAKFVTTHKDLLKADVILISDTAMLSLE
                     NPSIDIGVRGLSYIEVEVTGPNRDLHSGVYGGAVANPITILARMIASCHDANNHITIP
                     GFYDGVVEATPAERALMAKAPYSEEEYKKDLGVSQLWGEKGYTTNERTGIRPTIELNG
                     IWGGYTGEGAKTVLPSKATAKISARLVPNQSSRIITEKLLNYFRTIAPPGVTVKAEEH
                     HGGEPYMTPIDSKAYRSAARAIEATFGKAPIPVRGGGSIPICSLFEKVLGIKIVFMGF
                     GLDSDNLHSPNEKFDLANFYKGIATIPYFHKFFAEK"
     gene            1326..2717
                     /locus_tag="VGQ51_18845"
     CDS             1326..2717
                     /locus_tag="VGQ51_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038706.1"
                     /translation="MSQLFRRLRHSLPSGAPLAALLASVAILSIAVILLEWPVLQHTH
                     GKFILPTDDAFVNVSVARNFSFYQVWGISQHSFQSASSSLLYPLILAPVFFITGAHLV
                     IVLALNFLVAMVFLVVLQRVLIRHGLPAGRQFIVLVEGMALTVLPLLVVSGTEYTLQL
                     LMVFLFMEALSKALSDPTVKTGLPWRVYILALVTVATRYEDILIIALATLLVNPLRGW
                     RTASKLACVAILPIVVFGGISLTKGSYFLPNALLIGPYPAYAIFLTFCALCAIIPLLG
                     SYLKLSVPEKTKAVPYLSLVLLAIMALPFATRNLSALGHFKRDCIRMYDEEYLTAGFV
                     HLYYYKSTVGIDEPGAVSWFSEGRKLDFTGIANDEVIRSKKHHSWSPLYADSLGRRSG
                     IRAAILTNPWFDPGELPKWNIIATWTIPDSSPGGEKVVNFFALNQYDTAGLRRNLHEY
                     QHLLPADVAVRYY"
     gene            complement(2740..3375)
                     /locus_tag="VGQ51_18850"
     CDS             complement(2740..3375)
                     /locus_tag="VGQ51_18850"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEV9038707.1"
                     /translation="MKKKTLMIMAAVLLMAGGQVYAQTFQWGVKAGGNLTNFLSSNTS
                     TYNSYSATAGWNAGVFTNFWFDSGQHFAIAPEILYTTAGAEVSAGTSGGSASATSKSH
                     LDLAYVMIPVFAKYKFNGGFYLETGPEINFNVSHGQYQSESVSSFTKGADFEWGLGLG
                     YQSPVGFGIDLRYNQGITNVANTSNATWSDVTLRNSGFMLDVFWAFFNNKK"
     gene            3577..3963
                     /locus_tag="VGQ51_18855"
     CDS             3577..3963
                     /locus_tag="VGQ51_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV9038708.1"
                     /translation="MPDKEKLRLDKYLWSIRLFKTRTEAAEACDKGKVKYNGLAAKAS
                     KNITVGDEYEVKTENKRWRIKVTALLDHRLQYSQAINYYIDITPAEEIERLQFQAASF
                     NTGKRQSKIGRPTKKQRRDLDGFLED"
     gene            complement(3960..>4359)
                     /locus_tag="VGQ51_18860"
     CDS             complement(3960..>4359)
                     /locus_tag="VGQ51_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038709.1"
                     /translation="KFVHQYYDWDGIVFNDIGGVSYFSDGKKLDILGLGNNDVAQERR
                     QQRNSPALIDSLGKAKNAKVAIIYEPRFRAYTKNWTKVASWDIPYPDPIGFYDSVTFY
                     AVNPHDAPMLMNNLRQYEPSLPKEIVVRYY"
BASE COUNT          983 a         1462 c          968 g          946 t
ORIGIN      
        1 aacgtgaagt tcatcataga aggagaggaa gagatcggca gtcccaatct cgcaaaattc
       61 gtcaccactc ataaagatct gctgaaggcc gacgtcatcc tgatcagcga caccgccatg
      121 ttaagtctcg aaaacccgag catcgacatc ggcgtccgcg gtctctctta tatcgaagtc
      181 gaggtcaccg gccccaaccg cgacctgcac agcggcgtct atggcggcgc ggtagccaac
      241 cccatcacca tcctcgcgag gatgatcgcc tcctgccacg acgcgaataa tcatattacc
      301 attcccggct tctatgacgg cgtcgtcgaa gccacgcccg ccgagcgcgc cctgatggcg
      361 aaggcgccct atagcgagga ggaatacaaa aaggatctcg gcgtctcaca gctctggggc
      421 gagaaaggct ataccaccaa tgagcggacc ggcatccgcc ccaccatcga gctcaacggc
      481 atctggggcg gctataccgg cgaaggcgcc aaaaccgtcc ttccctcgaa ggcaaccgcc
      541 aagatctccg cgcgtcttgt tcccaaccag tcgagccgta tcatcaccga aaagctgctc
      601 aactatttcc gcaccatcgc acctcccggc gtcaccgtca aagccgaaga gcaccacggc
      661 ggcgagccct acatgacccc catcgacagc aaagcctacc ggtccgcggc ccgcgccatc
      721 gaagcaacct tcggcaaggc ccccatcccc gtccgcggcg gcggcagcat ccccatctgc
      781 tcgctcttcg aaaaagtgct cggcatcaag atcgtcttca tgggtttcgg cctcgacagc
      841 gacaacctcc acagccccaa cgaaaagttc gacctcgcga acttttacaa gggcatcgcg
      901 acgatcccgt atttccataa atttttcgcg gaaaaataga cgcgtgtctg agcagcgact
      961 ggggttaccg cccttccttc accgacctcc cctccccctg cgtcacccaa tgcacactat
     1021 cctgcgacga ctgtatcaaa taaacatact cctcccgcga acgaaaaaag gctcccgacc
     1081 tttcaatccc gccaccgtca gcacatattc atcggcctga tccgcgatca aaaaccctcc
     1141 acccggcgac caccggcaaa cattcatcgc cgatttcttc cctttattcg ctgaatccgt
     1201 agcctccgcc cgccacgtcc caacaaaaac atctaatacc gtaattggcg tccgcctgtc
     1261 tccatccccg ctcgcctatt gcagcctcat gtaatatatt agcccttcat taaaccttct
     1321 cccgtatgtc gcagcttttc cggcgtcttc gtcattcgct accctccggc gcaccgcttg
     1381 cagccctttt ggcctccgtc gccattctta gcattgcggt catcctcctc gaatggcccg
     1441 tcctccaaca tacgcatggc aaattcatcc ttccaacaga tgacgctttc gtgaatgtca
     1501 gcgtcgcgcg aaacttctcc ttttatcagg tgtggggaat ctcgcaacat tcgttccagt
     1561 ccgcatcttc gtccttatta tacccgctga tcctggcgcc tgtcttcttt atcaccgggg
     1621 ctcatctggt gatcgtcctg gcgctcaact tccttgtggc aatggtgttt ctggtggtcc
     1681 tgcagcgtgt cctgatcaga cacggtctgc ccgccggcag gcaattcatc gtattggtgg
     1741 aggggatggc gcttacggtt ttgcctcttc ttgtagtcag cggcaccgaa tatacactcc
     1801 agcttctcat ggtcttttta ttcatggagg cgttgtcaaa agccctttcc gacccgacag
     1861 tcaaaaccgg gttgccgtgg cgtgtctaca tcctcgcatt agtaacagtc gctactcgtt
     1921 atgaagacat cctgatcatc gcccttgcca ccctcctggt aaacccgctg cgcggatgga
     1981 ggacggcgtc aaagctggct tgcgtcgcga tccttcccat agtcgtattc ggtggcatca
     2041 gcctgacaaa aggcagctac ttcctgccca atgcgttgct gataggaccc tatcccgcgt
     2101 atgccatctt cctcacgttt tgcgcgctgt gtgccatcat cccgttgctg gggtcttatt
     2161 tgaagctatc cgtcccggaa aagacaaaag ccgtacccta tctttctctc gtcctgctgg
     2221 ccatcatggc ccttcccttc gccacccgca atctctccgc gctgggccat ttcaaacgcg
     2281 attgtatacg catgtacgat gaggaatacc tgaccgccgg cttcgttcat ctttattatt
     2341 acaagtcgac agtgggtatc gacgagccgg gtgccgtatc ctggttctcc gagggccgca
     2401 agctcgattt caccggcatt gccaacgatg aggtcatccg cagcaaaaag catcatagct
     2461 ggtccccgct gtatgccgac tcgctgggcc ggaggagcgg catccgcgcc gccatcctca
     2521 cgaacccctg gttcgacccc ggtgagctgc ccaaatggaa tatcattgcc acctggacga
     2581 tacccgactc cagtccgggc ggcgaaaagg tcgtcaactt ttttgccctc aaccagtacg
     2641 acaccgccgg tctccgccgc aatctccatg aatatcagca cctcctgccc gccgatgtgg
     2701 cggtgaggta ttactgaccg gggccgtcgg cgcgatcagc tacttcttat tgttgaagaa
     2761 ggcccagaac acatccagca taaacccgct gttgcgcaaa gtcacatcgc tccaggttgc
     2821 attgctggta ttagcgacgt tggtgatccc ctggttatac cggagatcga tcccgaaccc
     2881 aaccgggctt tgatacccca aaccgaggcc ccattcaaaa tccgctcctt tggtaaagct
     2941 gctgacgctt tcgctctggt actgcccgtg gctgacgtta aaattgatct cggggcccgt
     3001 ctccagataa aacccgccgt tgaacttata cttcgcgaat acgggtatca tcacataggc
     3061 caggtccaga tgacttttcg aagtggcgct cgcactgcca cccgacgtac cagcacttac
     3121 ttcggcccct gccgtcgtat acaggatctc cggcgcaatg gcgaaatgct gcccgctgtc
     3181 aaaccagaaa ttcgtgaata cacccgcatt ccagccggct gttgcggaat aggaattata
     3241 ggtggaagtg ttgctcgaca ggaaattcgt caaattaccc ccggccttta ccccccattg
     3301 aaaggtctgc gcatacactt gccctcccgc catcagtaat accgcggcca taatcattaa
     3361 tgtcttcttt ttcatatacc tagtttacta aaccccattc aaatcttatg ccccgggccc
     3421 ccgcgccaca tataccaccc cacccagccg ccccataggc gcccgcctcc gaaaacccgc
     3481 gccgccaccc cgtcccgccg ccccacgcac cgccacctca aaagccgccc cccaggcggc
     3541 cacaccccat tccagtggat ttcataattt tgctccatgc ctgacaagga aaagctccgt
     3601 ctcgataaat acctctggtc gatccgcctc ttcaaaaccc gtaccgaggc cgccgaagcc
     3661 tgcgacaaag gcaaggtcaa atacaacggc ctggccgcca aggcctccaa aaatatcacc
     3721 gtcggcgacg aatatgaggt aaagacggaa aacaaacgct ggcggatcaa agtcaccgcc
     3781 ctgctcgacc accgcctgca atattcgcag gcgatcaatt attacatcga catcacgccc
     3841 gccgaagaga tcgagcgtct ccagttccag gcagccagct tcaacaccgg caaacgccag
     3901 agcaagatcg gccgccccac caaaaaacag cggcgcgacc tcgacggttt cctggaagac
     3961 taataatatc taaccacaat ttccttcggc aacgacggct catactgccg taaattattc
     4021 atcagcatcg gcgcatcatg cggattcacc gcataaaaag tcaccgaatc ataaaaccct
     4081 atcgggtccg gataaggaat atcccacgac gccaccttcg tccaattctt cgtataggcc
     4141 cggaatcttg gctcataaat gatcgcgacc ttcgcattct tcgccttccc cagactatcg
     4201 atcagtgccg gagaattcct ttgctgccgc cgctcctgcg ccacgtcatt attccccaac
     4261 ccgagtatat ccagcttctt cccatccgag aaatatgaaa caccaccgat atcgttgaac
     4321 acgatcccat cccaatcata atattgatgt acgaacttc
//
LOCUS       DASYSV010000605        16493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_248034, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000605 DASYSV010000000
VERSION     DASYSV010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 16493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16493
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248034"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VGQ51_18865"
     CDS             <1..693
                     /locus_tag="VGQ51_18865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9038710.1"
                     /translation="VLKEKSRVRRLLGYLPQEFGVYPRISAEQMLDHVARLKGVDRAG
                     ERKELVDGLLNKVNLYKDKRKRLGTYSGGMKQRWGIAQALIGNPRLIIVDEPTAGLDP
                     AERNRFYNLLSHLGEQTIVILSTHIVEDVSTLCSRFAIICLGEVLYTGEPDAAIAALE
                     GRVFTKQIPRNEIQRYRADYPIISTQLKAGHLYIRIAADQHPGNGFEPAIPNLEDVYF
                     SHIATKMDVNTL"
     gene            712..4083
                     /locus_tag="VGQ51_18870"
     CDS             712..4083
                     /locus_tag="VGQ51_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038711.1"
                     /translation="MLSEIFHFEVRYRLRRPAFWVYFIFVFLATAWAFANGDLARQDQ
                     EFINSPVSLANFQTVMTIFLMLVSATIMGTPLYRDLEHNTKEYYLSYPITRAGYFWGR
                     YSGSFVFVALVGAAMPLGAMLGSQLGPLFGWQPATSYEHSPQWFYWQPYFFQVLPNLF
                     FTSSLFFGLVAVLRNVKVIYSSGMFLFLGYIIANFFLHNIHDPKVIYLSDPFNFNGLR
                     SATSGLLPERLNGHTVPMTGLLLDNRLLWTGVGLAGLLLTWWRFNFERFFGGRRTRTP
                     AGTPPAYTPETATRATKPVTGPGSAIGSGFAIEPGSAGVNPSYYRKTLLSLTRIELLN
                     LVRDGYLWIILTGGLIFMGFIFWHAPQPWGITDYPRTSVFMDAFSQNLLFFLFVIIVF
                     YTGESVHRERLTRYHFINDTLPPATWVFNCSKLLSLLCFGFFLAILPVGLGLAVQGLK
                     GYPYLNLSQYVSSEFVAILPKMAEMVLFAYAIHISVNNKFAAHGIAIAIWTLVLIAYT
                     FNYFDYNLLLYSNTPPYWASDMDGIGHLARSVSWYNFYWTIAGAVMVVLASLFYARGT
                     RSAARERIILARQRFHGPARAGFGVLFLIYLAVGSFIYYTVSYENEYLTPWEKQERAA
                     ITERQMKKFEHLPQPVATGLRLHIDLYPDRQQETTRAEVTLVNKGRIPIDSLLVDGDR
                     LDFELDYRGARLPYTCPLYYPRGKFDLFRPRLQPSDYRFYRLPGTLLPGDSAQVEVRS
                     NVAFHGFLNGFYGGNILHNGIVTAGNLPGLGYDDDDELRRNDIRKEHSLPEKNPQDTP
                     QDDSVGRLTRVNDITSGLIPLDITVSTSADQWAVAPGHLEKEWSEGGRHYFHYVQPPP
                     GVYIPFVIASARYSLLKDTVQLESGRKVDITIFYHRTNDLNLSHFIAAIKDGLHFYSR
                     AFGPYPFDYLYLVETPNYGPFSMSLPGMLAFAEPNTGWNGGFRARKVLDYAYYNTAGL
                     LAHQWWGQQVAPNNTVGSPLLSDGLSQYSALLLMRQRFGDTTVADLLQRLRGDYDWGH
                     RTNRTAENDLLHANRPYLWTAKAGISLYDLGRRMGTDSLDAALHDFLNTWAFRKEGPY
                     AGAPDLYKTLSRHIPDSLRTWFRDTWEKPGPPTDR"
     gene            4201..4836
                     /locus_tag="VGQ51_18875"
     CDS             4201..4836
                     /locus_tag="VGQ51_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV9038712.1"
                     /translation="MGINDRKQRQKEEVRASILATAWDMVLAEGWPSFSIRKIADAIE
                     YSVPVIYSHFENKDAILLEFNKKGFQLLAESMAVAKAGQSDPANQIRTMGRAYWDFAF
                     QNKEYYQLMFGLGIPTCETASKIPALASFAEVIIGSIRAMVAPGKQPSFDPWLKYQSF
                     WSMLHGLVSIKMTSLSGSEKANNPHGLLPQQVLEDVMCSFIRGIQASENAN"
     gene            4858..5487
                     /locus_tag="VGQ51_18880"
     CDS             4858..5487
                     /locus_tag="VGQ51_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV9038713.1"
                     /translation="MSLLESLQWRYATKRMTGERLPQEKVDIVLEAARLAPTSSGIQP
                     FAILQITDRQLLDKIRPIAFNQPQITEASHLLVFAAWSDVTAAHIDAVYDRIARERNL
                     PEGAQSAYVDNLKKRFDQMPAEKRAAFAANQSYIALGVAITAAASEKIDATPMEGFQN
                     AALDELLGLKEKGLRSVTLLALGYRDTTNDRLVHLKKVRRPKEELIIEI"
     gene            5612..6184
                     /locus_tag="VGQ51_18885"
     CDS             5612..6184
                     /locus_tag="VGQ51_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV9038714.1"
                     /translation="MKHIILSTATFLLVATGVTAQEWNMDKAHSQIMFVVEHMGINQI
                     TGNFGTVSAKFTASKDDFSDAAIEFTADINSINTGNEQRNNHLKSDGFFNAAQYGTLT
                     FKSTSFTKTGDKTYRLSGDLTFHGVTKPVTLDAVYNGSIVNPMSKKTTAGFKVTGVIK
                     RTDFGVGTSFPTTIVSDEVRLDANTEFIKS"
     gene            6186..6719
                     /locus_tag="VGQ51_18890"
     CDS             6186..6719
                     /locus_tag="VGQ51_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV9038715.1"
                     /translation="MKQAFTPLLAVFILAGSAFTLVSADNWKIADGHSITFSTDEASG
                     IFKDFKGSIVFDEQNPAASKFDVTIDVASVNTGNGLQNKHLKSDEWLDAAKYPLIHFT
                     SQKVVKTSSGYQVTGNLDMHGAKKPLTIPFTFKRTATGGLFTGAFNVNRNDFQVGKPG
                     GEVSDQIKLEASVPVTK"
     gene            6726..7115
                     /locus_tag="VGQ51_18895"
     CDS             6726..7115
                     /locus_tag="VGQ51_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038716.1"
                     /translation="MLNKSLILGIVSGVLAGIAGILYARVYYKINEADFSAVVSSMKI
                     IAASLVAGILAGIGYTVLDRLLKKKGEIVFNFLFTILSFASLLVPIAYKLPLNLETPD
                     LFPGMVIPMHFFPALSWYTLKPLFIRK"
     gene            7214..7594
                     /locus_tag="VGQ51_18900"
     CDS             7214..7594
                     /locus_tag="VGQ51_18900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9038717.1"
                     /translation="MNKRVLILDDDLDILQICTIVLKKKGFDVQTLNNSNQVVSLVKD
                     FQPDVILMDNWIPGPGGIEATRLLKLSPETQEIPVIFFSANSNVTQLAREARADYFLQ
                     KPFDITELEAIVQMAIRQGVEPAK"
     gene            7705..8925
                     /locus_tag="VGQ51_18905"
     CDS             7705..8925
                     /locus_tag="VGQ51_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEV9038718.1"
                     /translation="MSTTFSAAAVATPVGRSQRVQSIDLLRGVVMILMALDHVRDYFN
                     DSAYLYDPTDLTHTTPALFFTRWITHFCAPIFMLLAGIAAWLYGKKNGPRALSFFLLT
                     RGIWLVFVELFIVTLGWTFNIHFTFYILQVIWAFGICMIILSALIRMRPTALLVTALV
                     LIFGHNLLDPIQFSGNGFPAVGWALLHRFQPFFYGNYGFFVGYPILPWLGILVLGYCL
                     GRLYAPDVDPARRRKTLLILGLSSIAGFLLFRAVNIYGDPAPWSSQRSAIYTLLSFFN
                     VTKYPPSLLYTLMTLGPGFLFLSFAEGPLNSFTSKIAVYGRVPFFYYIVHIYALHGLA
                     VLAAMWQGHAASDMITVNNWVTGNPLLKGYGYGLGVVYTVWIGIVLLLYPLCRWFDGY
                     KRSRQAEYKWLSYL"
     gene            complement(8916..9482)
                     /locus_tag="VGQ51_18910"
     CDS             complement(8916..9482)
                     /locus_tag="VGQ51_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEV9038719.1"
                     /translation="MQDRLLVIGGSAGSLQVVISILKGMGKDYPWPVLIVLHRTNSFE
                     SSLEELLSSRSHLSIKEVEEKEPLVPGRVYVCPADYHVLLEQDHTTSLDYSERVNFSR
                     PSIDVTFRSAADVYGQALIALLLSGGNADGSDGMAYVREKGGMILLQDPQTAEVPYMP
                     QQVLLRMTVDVIASAGELPSLVRGLPYR"
     gene            complement(9472..10302)
                     /locus_tag="VGQ51_18915"
     CDS             complement(9472..10302)
                     /locus_tag="VGQ51_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEV9038720.1"
                     /translation="MEINPIQTEELELLLNDISTQYGYDFTEYSRASLQRRVQRLFAV
                     DKFPSFAEFRYRLRSDGHYFSRFVEEITVNVTEMLRDPSFYRTLRTTILPILATHPFI
                     RIWHAGCSTGEEVYSMAILLKEMGILHKSLLYGTDINPLVIDRARKGIFPMSQMQKYS
                     ENYIQAGGVKDFSTYYTANYHLAKFDSSLTGRMIFSTHNLVSDFSFNEFHLILCRNVL
                     IYFERELQTKVFRLFDYSLENFGYLALGSKESLRFSPMAGRYRQVGKEKIWRKVSDAG
                     "
     gene            complement(10305..13919)
                     /locus_tag="VGQ51_18920"
     CDS             complement(10305..13919)
                     /locus_tag="VGQ51_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV9038721.1"
                     /translation="MNSTSKPRLLRNLQIGFGLSLLILVVTSVASYSSIRNLLLGSRL
                     VDHTDSVISELNMTLSTLKDAETGQRGYLLSNDTAFLRPYWGSRERSLMLLDRVQRMT
                     LDNPIQQQNCKELKDLIFNRLAIMQEVIEEKRKTGSYTATSLEKGREYMNEVRDVVQR
                     MQDEEHQLLVIRTEKVESYASYAPVLIIAADLMAMLLTVFFYGRVHRDFLERHQLYSE
                     LEGKERDTARRIEIISGIADQISEGHYQTRVSDEQKDALGGLSGALNRMAESLDSAFR
                     LLSDKEWMKAGMARLSEVMAGETGMQHLSTQTLAFIAEYVGCEVGAFYVLDSGTGLLG
                     LSGNYALAAGGRKSFRIGDGLVGQVAAEGRRILFTDLRPSDFVISLAAGDIRPRTIIA
                     FPIVHEKIVTGVIELGTTGEFSVRELEFFDNIAEAIGMAVASIESRLRLQELLEQTQA
                     QTEELQAQHSELEALNLELETQTEKLQVNEEELKVQQEELLQTNHELEERSRTLEEKN
                     QLILIRNLDIQKKAEELALTTKYKSEFMANMSHELRTPLNSILLLSRLLVENTGKNLT
                     DEQTEYARVIQNSGHGLLQLIDEILDLSRIESGKLQLEHTIVSVREVLDDMRMLFGPM
                     AKDKNLQLNILVDEGTPAQMETDKMRLEQILKNLLANAFKFTHKGSITLQVRPSGREH
                     FLEFIVRDTGIGIPEDRHQMIFEAFQQADGSTRRKYGGTGLGLSISREFSRLLGGEIS
                     LTSHPDEGSEFMVTLPEFKTEAVGEPVAGSYPQNANVTGVSAQARPVEEVSSGLGYTL
                     PEAPSEIPDDRSDLHAGDRVLLIVEDDTFFAQSLLEFSRKKGYKGIVAVSGDTAIRLA
                     RQYQPMGILLDIQLPVKNGWEVMEELKKDPGTRSIPVHIMSSFEVRNESLLKGAVNFV
                     HKPLAVEDMEGVFGKIESLLRHNARKVIIVEDDIRHAQALAYFLGTHDLRMEIAPTIE
                     QSSEVLQKDGLHGVILSRENGKEESDTLEMIRRHPVFENVPIIVFTGMNTTKTEEARL
                     RQFADSIVVKTAHSYQRVLDEVSLFLHVVGEGPGRNNGNAPGRLGALDEVLKGKTVLV
                     ADDDVRNIFSLTKALERHKMRVVSAMDGQEALTVMESEPVDIVLMDMMMPEMDGFEAI
                     TRIRRKPRQRNLPILAVTAKAMAGDRERCIQAGASDYISKPVDIDQLLSLLRIWLYEK
                     "
     gene            complement(13924..15069)
                     /locus_tag="VGQ51_18925"
     CDS             complement(13924..15069)
                     /locus_tag="VGQ51_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEV9038722.1"
                     /translation="MILIVDDKPENIFSLKTILELHSFPTDTALSGEEALKKILRNAY
                     ALIILDVQMPGMDGFEVAEAISGYSKARDIPILFLSAASTDKKFITKGYSAGGMDYIT
                     KPIDPDILLLKVKTFYRLYEQNRELNQIQASLRSEIEFRKRVEGELQTAVLKRDEFIS
                     IASHELKTPLTTIKAYVQLLDRSIPGDEPTRMYVERALVQVQKLDHLIVDLLDLSKIE
                     SGKLKFNKKEFSFESTLSNAVEMIRQTYPDYHIVRKGGAEVELYGDEVRIEQVIINYL
                     TNAVKYSPNVKEIQIEAAVEPAGRVSVWVKDFGIGIPKDHQSNIFSKFYRVDETSGQF
                     QGLGIGLYICAEILRRHDGDYGVESEPGKGSTFYFSIPTNNRTTTPS"
     gene            15279..16058
                     /locus_tag="VGQ51_18930"
     CDS             15279..16058
                     /locus_tag="VGQ51_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038723.1"
                     /translation="MQPILNRYRRTRLRDYFHRAGDLVAFMLRFFREVFVPPYEWREV
                     FYQCYLVGYKSTFLISLTAFIMGVVLTIQSRPSMAAFGAISLIPGMVSVSVIREIGPV
                     ITAIICCGKVGSRMGAELGSMRVTEQIDAMEVSAINPFRYLVVTRTVATTLMIPLLTI
                     IADIVAFLGAWLAMNIHDTSTLRHFFQAAISHLGFLDIFPAVIKTFFFGFVIGIIGCF
                     KGFRARRGTESVGLAANSAVVASSIAVFIIDMLAAQITDLL"
     gene            16055..>16493
                     /locus_tag="VGQ51_18935"
     CDS             16055..>16493
                     /locus_tag="VGQ51_18935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9038724.1"
                     /translation="MNYIKNDTVPIPAADTSDQPDIDPQAMPDASDQPEIATQPEIAP
                     QPTPDTSVQPDETAALASVDEEPVVLIRGLVKSFKDHEVLRGVDLHVKKGENLVILGK
                     SGTGKSVLIKCLVGLEWPDAGTISIFGEDIAALSYNKMNALRVR"
BASE COUNT         3653 a         5025 c         4004 g         3811 t
ORIGIN      
        1 gtactgaaag aaaagtcgcg ggtccggcgg ttgctgggtt atctccccca ggaattcgga
       61 gtctaccccc gtatctccgc cgaacaaatg ctcgatcacg tggcccgtct caaaggcgtg
      121 gaccgggcag gcgagcgaaa agagctggtc gacgggctgc tgaataaagt caatctttac
      181 aaagacaaaa gaaaacgtct cgggacgtat tccggcggaa tgaaacaacg ctggggcatc
      241 gcgcaggcgc tgatcggaaa cccgcggctg atcatcgtcg acgagccgac cgcaggactc
      301 gaccccgccg aacgaaaccg tttttacaat ctgctgagcc atctcggcga acagaccatc
      361 gtcatcctct cgacccatat cgtcgaggac gtgagtaccc tatgctcccg ttttgccatc
      421 atctgcctgg gagaagtgct gtacaccggg gagccggacg ccgccatcgc cgcattggaa
      481 ggaagggtct ttaccaaaca aataccgcgt aatgaaattc aacgctaccg ggccgattac
      541 ccgatcatca gcacacagct gaaggccggg cacctgtata tccgcatcgc tgcggatcag
      601 caccccggca atgggttcga acccgcgata cccaacctgg aagatgttta tttcagtcat
      661 atcgccacca aaatggatgt gaacacactc taacacgcca aactctttcc tatgttaagt
      721 gagatatttc atttcgaagt ccgataccgg ctccgccgac ccgccttctg ggtctatttc
      781 atcttcgttt tcctggcaac ggcctgggca ttcgccaacg gcgatctcgc gcggcaggac
      841 caggaattca tcaactcccc ggtctcactc gccaatttcc agacggtgat gaccatcttc
      901 ctgatgctgg tcagcgccac gatcatgggc acacccctgt atcgcgatct cgagcacaac
      961 acgaaagaat actatctctc ctaccccatc acccgggcgg gttatttctg gggccgctat
     1021 tccggctcct tcgtattcgt cgcgctcgtc ggcgcggcaa tgccattggg cgctatgctc
     1081 ggaagccagt tgggaccgct gttcggatgg caacctgcta ccagttatga acattcccca
     1141 caatggttct actggcagcc ctacttcttt caggtgctgc ccaatctctt ctttacctcc
     1201 tcgctatttt tcggtctcgt cgccgtcctc cgcaatgtaa aggtcatcta tagcagcggc
     1261 atgttccttt ttctcggata tatcatcgcc aattttttcc tgcacaacat ccatgacccg
     1321 aaggtgatct atctgtccga tcctttcaat ttcaatggcc tccgctcggc aacgagcggc
     1381 cttttacccg aacgtctcaa cggacacacg gtccccatga cgggtctcct gctggacaac
     1441 cggctgctgt ggaccggcgt aggcctcgca ggcctgctgc tcacgtggtg gagattcaat
     1501 ttcgaacgct tcttcggtgg ccgccggaca cggactcctg ccggcacacc accggcttat
     1561 acgcccgaaa ccgccacaag agctaccaaa cccgtcactg gacccggctc cgccattgga
     1621 tccggcttcg ccattgaacc cggctctgcc ggggtcaatc ccagctacta tcgaaaaaca
     1681 ttgctcagcc tcacccggat cgagctgctc aacctcgtcc gtgacggata tctctggatc
     1741 atcctcaccg gcggtctcat ctttatgggc ttcatcttct ggcacgcccc gcaaccatgg
     1801 ggcattaccg actatccacg aaccagcgtc ttcatggacg ctttctccca gaacctgcta
     1861 ttctttctct tcgtgatcat cgtattctat accggcgaga gtgttcaccg cgaacggctg
     1921 acacgctatc atttcatcaa cgacaccctc cctcccgcaa cctgggtctt caactgctcc
     1981 aaactgctga gcctgctttg ttttggcttc ttcctggcga tccttcccgt cggactcggt
     2041 ctggcggttc agggattgaa gggctatccc tatctcaacc tgtctcagta cgtctcttcc
     2101 gaattcgtcg ccatcctccc taaaatggca gagatggtct tgtttgccta tgccattcat
     2161 atctccgtca acaataaatt cgccgcccat ggcatcgcca tagctatctg gaccctcgtc
     2221 ctcatcgcct atacgttcaa ttatttcgat tacaacctcc tgctatattc caacacaccg
     2281 ccttattggg cctccgatat ggatggcatc ggacacctgg cgcgatccgt ctcctggtac
     2341 aacttctact ggaccatcgc cggcgccgta atggtcgtgc tcgcctcctt gttctatgcc
     2401 cgcggtacgc gaagcgctgc ccgtgaaagg atcatcctcg ccaggcaacg gttccatgga
     2461 cccgcacgcg ctggtttcgg agtccttttc ctgatctatc tcgccgtcgg cagtttcatc
     2521 tactatacgg tcagctacga gaacgaatat ctcaccccct gggaaaaaca ggaacgcgcg
     2581 gcgataaccg aacgacaaat gaaaaaattt gagcacctgc cccagccggt ggcaaccgga
     2641 ctcaggttgc atatcgatct ttatcccgac cgacagcagg agacgacaag agcggaggtc
     2701 acgctcgtca acaagggacg catccctatc gatagcctcc tggtcgacgg cgaccggctc
     2761 gattttgagc tggattaccg cggcgcccgg ctcccctaca cctgccccct ctattacccg
     2821 cgcggcaaat tcgatttgtt ccggccacgg ctccagccct ccgactatcg cttctatcgt
     2881 cttcccggaa cgctgctgcc gggagacagt gcacaggtgg aagtccggtc gaatgtcgcc
     2941 tttcatggtt ttctgaatgg tttctacggc ggcaatatcc tgcacaacgg tatcgtcacc
     3001 gccggcaatc tcccgggact tggttacgat gatgacgatg aattgcggag aaatgacatc
     3061 cggaaagaac acagcctgcc ggagaaaaac ccgcaggata caccgcagga cgattccgtc
     3121 ggccgcctca cccgggtcaa tgacattacg tccgggctga taccgctgga tatcactgtc
     3181 agcacgtccg ccgatcaatg ggcggtagcc ccgggccatc ttgaaaagga atggagcgag
     3241 ggtggacggc actattttca ttatgtccaa ccccctcccg gcgtctatat accttttgtg
     3301 atcgcgtccg cgcggtattc cctgttgaag gatacggtgc agctggagtc cggccgcaag
     3361 gtcgacataa cgatcttcta tcaccgcacc aatgatctca acctgtccca tttcatcgcc
     3421 gcgataaagg acggtctgca cttttattcc cgcgccttcg gcccctatcc cttcgattac
     3481 ctgtacctgg tagaaacccc caactatggg ccgttcagca tgtccttgcc gggaatgctg
     3541 gcgttcgccg agcccaatac gggatggaat ggcggcttcc gcgccaggaa ggtcctcgat
     3601 tatgcgtact ataatacggc tggtttgctg gcccaccaat ggtggggtca gcaggtcgct
     3661 ccgaacaata ccgtaggctc ccctctgctc tccgacgggc tttcgcaata ctcggcctta
     3721 ctgctcatgc gtcaacgctt cggagacaca acggtggccg atctgctgca aagactgcgt
     3781 ggggattatg actggggcca ccgcaccaat cgcaccgccg aaaacgatct cctgcatgcc
     3841 aatcgtccct atctctggac ggcaaaggcc ggtatctcgt tatatgacct cggccggcgc
     3901 atgggaaccg atagtctcga tgccgccctg cacgattttc tgaatacctg ggcattccgg
     3961 aaagaaggcc cctatgccgg cgctcccgat ctgtacaaga ctctgtcccg acacatcccc
     4021 gattcgctcc gaacctggtt ccgcgatacc tgggaaaaac ccggaccgcc taccgaccgc
     4081 tgacccggct cccgccgccg cgccatgaaa aaccgcccac cccataaaaa tttttttggt
     4141 ggaataaaat aagggcgtaa tttcctaacg ctgttacaaa aagtaacaga cctaggacaa
     4201 atgggtatca acgatcgaaa acaacgacag aaagaagagg tccgcgccag catcctggcc
     4261 accgcctggg acatggtcct tgccgaagga tggccctcct tctctatccg taagatcgcc
     4321 gatgccatcg aatacagcgt tccggtcatc tatagtcatt ttgaaaataa agacgctatc
     4381 ttgctggaat tcaataaaaa gggattccaa ctgctagccg aatcaatggc tgtggcgaag
     4441 gccgggcagt ccgaccctgc caaccagatc aggaccatgg gacgggcata ttgggacttt
     4501 gcctttcaaa acaaggaata ctaccagctg atgttcgggc tcgggatccc tacctgcgag
     4561 accgccagta agatccctgc actggcctcc ttcgcggagg tcatcatcgg aagcatcagg
     4621 gcgatggtag ctcccggaaa acaaccctcc ttcgacccct ggttaaaata ccaatctttt
     4681 tggtccatgt tgcatgggct cgtgtcgatc aaaatgacct cgctcagcgg ctccgaaaag
     4741 gcgaataacc cccatggtct gttaccacag caagtccttg aagatgtcat gtgcagcttt
     4801 atccgtggca tccaggcatc ggaaaacgca aattaaacac caactaaaaa aaattatatg
     4861 agcttactgg aatctctcca atggcgctat gcgaccaaaa ggatgaccgg ggaacgactc
     4921 ccacaggaaa aagtcgatat tgtgctcgaa gcggcccggc tggcgccaac cagcagtggc
     4981 attcagccgt ttgctatcct gcagatcacg gacaggcaat tgctggacaa gatacgccct
     5041 atagccttca accagccgca gataaccgaa gcatcgcatc tgctggtctt cgccgcctgg
     5101 agcgatgtaa cggccgcgca tatcgatgct gtttatgatc gcattgcccg tgaacgcaac
     5161 cttcccgaag gagctcaatc cgcctatgtc gacaacctga aaaagaggtt tgaccaaatg
     5221 cccgctgaaa aacgcgctgc ctttgcagct aaccaatcct atatagcact tggcgtggcg
     5281 atcacggccg cagccagcga aaagatcgac gccactccga tggaaggctt tcagaatgca
     5341 gcgctcgacg agttgctggg tctgaaggag aaggggcttc gtagcgtcac cctcctggcc
     5401 ctcggctacc gcgataccac aaacgaccgg ctcgtacacc tgaaaaaagt ccgccgcccc
     5461 aaagaagaac tgatcatcga aatttaaaat ttagaaccaa cccggacggg caatagcccg
     5521 tcggccgaag gcccatcatt aactgtgcga ggggaatggg cattatcgga accaaccaat
     5581 ccccaagcac taacaaaaac aaacgcacac aatgaagcac atcatcctca gtacagcaac
     5641 cttcctattg gtggcgaccg gcgtcaccgc gcaggaatgg aacatggaca aagcacactc
     5701 ccagatcatg ttcgtcgtcg aacatatggg catcaatcag atcacgggta atttcggtac
     5761 cgtttcggca aaattcacgg cttccaaaga cgatttctcc gatgccgcga tcgagttcac
     5821 cgccgatatc aacagcatca acaccggcaa cgaacaacgc aacaaccatc tcaagagcga
     5881 cggattcttc aatgccgcgc aatacggcac gcttaccttc aagagcactt ccttcacaaa
     5941 gacgggggac aagacctatc gtctctcggg cgacctgacc tttcacgggg tgaccaaacc
     6001 cgttacgctc gacgccgttt acaacggatc gatcgtcaac ccgatgagta agaaaacgac
     6061 cgccggcttc aaggtaaccg gggtcatcaa acgcaccgat ttcggcgtgg ggacttcctt
     6121 cccgaccacc atcgtcagcg atgaggtaag gctcgacgcc aatacggaat ttataaaaag
     6181 ctgatatgaa acaagcattc actccgctcc tggccgtgtt catcctggcc gggagcgctt
     6241 tcaccctggt ctccgcggac aattggaaaa tagccgacgg ccattccatc acgttttcca
     6301 ccgacgaagc atccggcatc ttcaaggatt tcaaagggtc catcgtcttc gatgagcaaa
     6361 accccgccgc gtcgaaattc gatgtgacca tcgacgtggc ttcggtcaac accggcaatg
     6421 gcctccagaa caaacacctg aagagcgatg aatggctcga tgcggcaaaa tatcccctca
     6481 tccatttcac ttctcagaag gtggtcaaaa cttcatccgg ctatcaggtt acgggcaacc
     6541 tggatatgca tggagcaaaa aaaccgctga ccatcccatt taccttcaaa cgcaccgcta
     6601 cgggcggact cttcacgggc gccttcaatg tcaatcgcaa cgattttcag gtgggcaaac
     6661 ccggcggaga agtcagcgac cagatcaaac tggaagcatc cgttccggtc accaaataac
     6721 ctgttatgct caataaatca ctaatactcg gcatcgtatc gggtgtcctg gcggggatcg
     6781 ccggcatcct ttacgcacgt gtatattata agatcaatga ggcggatttc tccgcagtcg
     6841 tctcttccat gaagatcatc gccgccagcc tggtcgccgg gatcctcgcc gggatcggct
     6901 acacggtcct ggatcggctc ctgaagaaaa agggggagat cgtctttaac ttcctgttca
     6961 ccatccttag ctttgccagt ctgctcgtgc ctatcgccta taagctgccg ctcaacctcg
     7021 agaccccgga tctcttcccc ggcatggtca tcccgatgca ttttttcccg gcgctttcct
     7081 ggtatacgct caaacccttg ttcatccgca agtaagaaga accggggccc cgtccgtacc
     7141 cacccaacca ccggaggcgg tttccatttg ctggcataga ttttatagca gatatttgtg
     7201 gtaatatccg gttatgaaca aaagagtact gatattggat gacgacctgg acattctgca
     7261 gatctgcacc atcgtcctga agaagaaagg tttcgacgtg cagaccctga ataactccaa
     7321 ccaggtggtc agcctggtca aagattttca accggacgtc atactcatgg acaactggat
     7381 cccgggtcca ggcggcatcg aggccacacg cctgctaaag ctctccccgg aaacacagga
     7441 gatccccgtc atttttttca gcgccaacag caatgtcacg caactggccc gcgaggcaag
     7501 ggccgattat tttcttcaaa aacccttcga catcaccgag ctggaagcaa tcgtccaaat
     7561 ggccatccgc cagggcgtgg aacccgcgaa ataaaccacg accagacacg gcccccgcag
     7621 gtctggacta cccgcatttt catgtctggc acatcgatat tttcagcgaa catcccatct
     7681 ttgcagtgaa aaacaccgct caccatgtca acaacgttct ccgcagcagc cgtcgccacc
     7741 cccgtcggac gctctcaaag agttcagtcc atcgatctcc tccgcggcgt cgtcatgatc
     7801 ctgatggcac tcgaccatgt ccgggattat tttaatgaca gcgcatatct ctacgatccg
     7861 accgatctga cacataccac ccccgcactt ttcttcaccc gctggataac ccatttctgt
     7921 gcccccatat tcatgctatt ggcaggcatc gccgcctggc tttatggcaa aaagaacggg
     7981 ccccgcgcct tatccttctt tctgctcacc cgcggtatct ggctggtgtt cgtagaattg
     8041 ttcatcgtca ctctgggctg gaccttcaac atccacttta ccttttatat cctgcaggtc
     8101 atctgggctt tcggcatctg catgatcatt ctgtcggccc tcatccgcat gcgcccgacg
     8161 gccttgctcg tcaccgccct cgtcctcatt ttcgggcata atctcctcga ccccatccag
     8221 ttcagcggca acggtttccc cgcggtcggc tgggcgctgc tccatcgctt ccaacccttc
     8281 ttctatggca attatggctt ctttgtcggc taccccatcc tgccctggct ggggatcctc
     8341 gtcctcggat attgcctcgg ccgtctatac gcacccgatg tcgacccggc ccgtagaaga
     8401 aagacgctgc ttatcctcgg tctttcatcg atcgcgggct tcctcctctt ccgggctgtc
     8461 aacatctatg gcgatcccgc accctggtcc tcgcaacgaa gcgcgatcta caccctgctt
     8521 tcttttttca acgtcactaa gtatccccct tcccttttgt atacactgat gacgctgggt
     8581 ccgggcttcc tgttcttgtc cttcgccgaa ggaccgctca actcctttac gtctaaaatt
     8641 gccgtatacg gccgcgtccc ctttttctat tatatcgtac acatctatgc gttgcacggt
     8701 ctggccgttc tggccgcgat gtggcagggt catgccgctt cggacatgat cacggtcaac
     8761 aactgggtaa ccggtaaccc ccttctcaaa ggatacggat acggtttggg ggtggtctac
     8821 acggtctgga tcggcatcgt ccttctcctc taccctcttt gccgttggtt cgacggctac
     8881 aaacgcagca gacaggcaga atacaaatgg ttgagctatc tgtaaggaag accccggacc
     8941 agggaaggca attccccggc agacgctata acatccactg tcatccgtaa aagcacctgc
     9001 tgcggcatat aaggcacttc cgcagtctgc ggatcctgca aaaggatcat cccgcctttc
     9061 tcccggacat aggccatccc gtccgaaccg tcggcattgc cgcccgacag caacagcgcg
     9121 atcagcgcct gaccatagac atccgccgcc gaccggaagg taacgtcgat gctcggacgg
     9181 ctgaaattca cccgctcgga ataatccagt gaggtcgtat gatcctgctc cagcaatacg
     9241 tgatagtccg ccgggcagac atacaccctt cccggcacga gcggctcctt ttcctccacc
     9301 tccttgatgg acaaatggct tcggctggag agcaactcct ccagcgaaga ctcgaagcta
     9361 ttcgtgcgat gaagcacgat gagtaccggc caggggtaat ccttccccat tcctttcagg
     9421 atggaaatga ctacctgcag actcccggcg gagcccccga tcaccaataa tctatcctgc
     9481 atcgctgacc ttcctccata ttttttcctt gcccacctgg cggtatcgtc ccgccatcgg
     9541 tgagaaccgc aatgattcct tcgaacctaa agccagataa ccgaaattct ccaaactgta
     9601 atcgaacagc ctgaagacct tcgtctgcag ctctcgctcg aaatagatca gcacattccg
     9661 gcaaaggatc aggtggaatt cgttgaacga gaaatccgac accaggttat gcgtcgaaaa
     9721 gatcattctg cccgtcaatg aggaatcgaa tttagccagg tgataattgg ccgtatagta
     9781 ggtagagaaa tccttcacac cgcccgcctg gatatagttc tcggaatatt tctgcatctg
     9841 gctcatcggg aatatcccct ttcgcgccct gtcgatcacc aatggattga tatccgtccc
     9901 gtaaagcagg gatttgtgca ggatacccat ctccttcagc aggatcgcca tcgagtagac
     9961 ttcctcccct gtcgaacatc ccgcatgcca aatgcggata aaaggatggg tggccagtat
    10021 cggaagaatg gtggtccgta acgtacggta aaacgatggg tcccgaagca tctccgtaac
    10081 attcaccgtt atctcctcga caaaacgact gaaataatgg ccatccgatc ggagccgata
    10141 ccggaattcc gcgaaactgg gaaatttatc cacggcgaac agcctctgca ccctgcgctg
    10201 cagcgatgcc ctcgaatact cggtaaaatc atatccatac tgcgtagaaa tatcattcaa
    10261 cagcagttcc agttcctcgg tttgtatggg gttgatctcc atcttcattt ttcatacaac
    10321 cagatccgta ataaagacaa cagctgatcg atgtccacgg gcttggatat ataatcggag
    10381 gcgcctgcct gtatacagcg ttccctgtcg cccgccattg ccttggcggt cacggcgagt
    10441 atcggtaaat tccgttgccg gggcttcctc cttatgcggg taatggcttc gaacccatcc
    10501 atctccggca tcatcatgtc catgagaacg atatccaccg gctccgattc catgacggtc
    10561 agcgcctcct gcccgtccat agccgagacg acacgcattt tatgccgctc caacgccttg
    10621 gtcagcgaga agatatttcg cacgtcatca tcggcgacca ggaccgtctt gcccttcagc
    10681 acctcatcga gtgcacccag cctgccgggc gcattcccat tatttctccc cgggccctct
    10741 ccgacgacat gcaggaagag ggacacctca tccagcaccc gttgatagga atgagccgtt
    10801 tttaccacga tactgtcggc aaactgccga agccgggctt cttcggtctt cgtggtgttc
    10861 atacccgtaa agacgatgat cggcacattt tcaaacacgg ggtgccgacg gatcatctcc
    10921 agtgtgtccg attcctcctt tccattttca cggctgagga tgacgccgtg caatccgtcc
    10981 ttctgcagga cctccgaact ctgttcgatg gtaggcgcta tttccatccg caggtcatgc
    11041 gttcccagga aataggccag ggcctgggca tgccgtatgt cgtcctccac gatgatcacc
    11101 ttccgggcat tgtgccggag aagactctcg atctttccga aaacgccctc catgtcttcc
    11161 actgccagcg gcttgtggac aaaattgacg gcccctttga gaagactctc attcctcact
    11221 tcgaaggagg acatgatatg gacgggtatg ctccgggtgc ccggatcttt tttcagctcc
    11281 tccatgacct cccacccgtt ctttaccgga agctggatat ctagcaggat ccccatcggc
    11341 tgatattgcc gggccagacg gatggccgta tccccgctga ccgcgacgat gcctttgtat
    11401 ccttttttcc ggctgaattc caggaggctc tgtgcgaaaa aagtatcgtc ttcgacgatc
    11461 agcaacaccc tatccccggc atgcagatcg ctcctgtcat cgggtatttc ggagggagct
    11521 tcgggcagcg tatagccaag cccggaagac acctcttcga caggcctcgc ctgggcagac
    11581 acccctgtca cattcgcatt ttgcgggtag gacccggcca ccggttcccc gacggcttcg
    11641 gtcttgaact ccggcagggt gaccatgaac tccgatccct catcgggatg actggtcagg
    11701 ctgatctccc cacccaacag ccgggaaaac tcacggctga tggaaagtcc cagacccgtt
    11761 ccgccgtatt tgcgacgtgt cgacccgtcc gcctgctgga aggcctcgaa gatcatctgg
    11821 tgcctgtcct cggggatacc gatacccgtg tcacggacga tgaactcgag gaaatgctcg
    11881 cgtccggagg gacgcacctg cagcgtgatc gatcccttat gagtgaactt gaaggcattc
    11941 gccagcaggt tcttcaagat ctgctccagc cgcattttgt ccgtctccat ctgggccgga
    12001 gtgccttcgt ccaccaggat attcagttgg aggttcttgt ctttggccat cggtccgaac
    12061 aacatgcgca tgtcgtcgag cacttcccgg accgatacga tcgtatgctc caattgcaat
    12121 ttcccggact cgatccgcga cagatcgagt atctcgtcga tcagctgcaa taacccatga
    12181 ccgctgtttt ggatgaccct tgcatattcg gtctgctcgt ccgtcaggtt ctttcccgta
    12241 ttctctacca gcagccgtga caacagcagg atggagttca gcggggttcg cagctcatgc
    12301 gacatattgg ccatgaattc cgatttgtat ttggtagtaa gggccagctc ctccgccttt
    12361 ttttgaatat ccaggttccg gatcaggatc agctggttct tttcttccag cgtccgggat
    12421 cgttcctcca actcgtgatt ggtctgcagc aactcttcct gctgcacctt cagctcttcc
    12481 tcgttgacct gcagcttctc cgtctgcgtt tccaattcga gattgagggc ctccagctcg
    12541 ctgtgctgcg cctggagctc ttccgtctgt gcctgcgtct gttccagcaa ttcctgcagt
    12601 cgaagccggc tttcgatact ggcgactgcc attccgatgg cttcggcgat attgtcgaag
    12661 aattccagct cacgcacgga gaactcaccg gtcgtcccca gctcgataac gcccgtgacg
    12721 attttttcat ggactatggg gaaggcgatg atcgtcctgg gccggatatc tccggcggca
    12781 agactgatga cgaaatcgga gggcctgaga tcggtgaaca gtatccgcct gccctccgcg
    12841 gctacctggc ctaccagccc gtcccctatc cggaagcttt tcctgccacc tgccgccaac
    12901 gcatagttgc cggacaatcc aagcaaaccc gtgccggagt ccaggacata aaaagcgccg
    12961 acttcgcagc cgacatattc ggcgataaat gcaagcgtct gcgtcgacag gtgctgcatg
    13021 cccgtctcgc cggccatgac ttcggacaga cgcgccattc ctgccttcat ccattcctta
    13081 tcggacaaca gacggaatgc agaatccagc gattctgcca tccggttaag cgcccccgaa
    13141 agcccgccga gcgcatcctt ctgctcgtcc gatacgcgcg tctgataatg accttccgag
    13201 atctggtcgg ctatacccga tatgatctcg atgcgtcgcg cggtgtcgcg ttccttaccc
    13261 tcaagttcgg aatacaattg atgtctttcc aggaaatccc ggtgaacacg tccgtaaaag
    13321 aataccgtga gcagcatggc catcaggtcg gcggcaatga tcagtacggg cgcatagctg
    13381 gcataagact ctaccttttc cgtccggatc accagcaact ggtgttcctc gtcctgcatg
    13441 cgctgtacga cgtcccggac ttcattcatg tattcgcgac ccttctccaa cgaggtcgcg
    13501 gtgtacgagc cggtctttcg tttctcttct atcacctcct gcatgatcgc gagccggttg
    13561 aagatcaggt ccttcaactc tttgcaattc tgctgctgga tgggattgtc caatgtcatg
    13621 cgttggaccc tatcgagaag catcagcgag cgttcgcgac ttccccaata tggacggaga
    13681 aaggccgtgt cgttggacag cagatagccg cgctgacccg tctctgcatc cttcaatgtt
    13741 gacagtgtca tattcagctc actgatcacc gagtcggtgt gatccaccag gcgcgagccg
    13801 agcagcaggt tcctgatact gctataggac gcgacggacg tgacgaccag gatcagcagc
    13861 gaaaggccga aaccgatctg cagattacgt aagagccttg gtttcgatgt actattcata
    13921 acgttatgaa ggggttgtcg tcctgttgtt cgtaggtatg gaaaaataaa aggtggaacc
    13981 tttccccggc tcgctctcga cgccatagtc gccgtcgtgt cttcggagta tctccgcaca
    14041 gatgtataac ccgatgccca gcccctggaa ctgcccggac gtttcatcca ccctgtagaa
    14101 tttgctgaaa atattcgact gatgatcctt ggggatgccg atgccgaaat ctttcaccca
    14161 caccgatacc cgcccggccg gttcgacggc tgcttctatc tgtatctcct tgacgttggg
    14221 ggaatacttg acggcattgg tcaggtaatt gatgatcacc tgctcgatcc gcacttcatc
    14281 gccgtacaat tcgacctccg ccccgccctt ccggacgata tggtagtcgg gataagtctg
    14341 acggatcatc tccaccgcgt tggaaagcgt ggattcaaac gagaactcct tcttattgaa
    14401 tttgagcttg ccgctttcta tcttggagag atcgagcaga tccacgatca ggtgatccag
    14461 tttctgcacc tgcacgaggg ccctctctac atacatcctc gtcggctcgt cccccgggat
    14521 gctgcgatcc agtaactgta catacgcctt gatcgtggtg agcggcgtct tgagctcatg
    14581 actggcgatg ctgatgaatt cgtcccgctt caagacggcc gtttgcagct cgccctctac
    14641 tcttttccga aactctatct cgctgcgcag gctggcctgg atctgattca attcccggtt
    14701 ttgctcatac agcctataga aggtcttcac tttcagcaga aggatgtccg ggtcgatagg
    14761 cttggtgatg taatccatgc caccggcgct atatcctttg gttatgaatt ttttatccgt
    14821 gctggcggcg gagagaaaaa ggatagggat atcccgggcc ttactgtagc cgctgatcgc
    14881 ctccgctacc tcgaacccat ccatacccgg catctgtaca tcgaggatga tcaaagcata
    14941 ggcattccga agtatcttct tcagcgcttc ctcgccgctc aaagccgtat ccgtgggaaa
    15001 ggaatgcaat tccaaaatgg tcttgagcga aaagatgttc tcaggtttgt cgtcgacgat
    15061 cagtatcatt tctccataca gtgattaaag tatgacgata aaggttaggt ccacgataag
    15121 gctaggaaca aaaataagtg tttatagagg gagatacagg ggaagaaatg tggaaatagt
    15181 tctacaattt ttgggttctg aacggcgtgc caattttttc cgttggggat tccagcctgc
    15241 aggccggttt ggagtgagat ttgaacttaa taccttgtat gcaacccatc cttaaccgat
    15301 atcggcgaac aaggctccgg gattatttcc atcgcgccgg cgacctggtc gctttcatgc
    15361 tccggttctt ccgggaagtg tttgtgccgc cctacgaatg gcgggaggtc ttttaccaat
    15421 gttatcttgt aggatataaa tcgactttcc tcatcagtct cacagccttc atcatgggcg
    15481 tggtgctgac catccagagc cggccctcga tggccgcctt cggcgccatc tcgctgatac
    15541 cgggaatggt atcggtatcg gtcattcgcg agatcggtcc ggtgatcacg gccatcatct
    15601 gctgcggtaa ggtgggttct aggatgggag ccgagctggg ctcgatgcgt gtcaccgagc
    15661 agatcgacgc catggaggtc tccgcgatca accccttccg ttatctcgtg gtgacacgca
    15721 cggtcgccac taccctgatg atccccctgc tgaccatcat cgccgatatc gtcgctttcc
    15781 tcggagcctg gcttgcgatg aacatccacg atacatcgac gctgcggcat ttcttccagg
    15841 cagctattag ccatctgggg ttcctcgata tctttccggc cgtcataaag acctttttct
    15901 tcgggttcgt catcggcatc atcggatgct tcaaaggttt tcgcgcccgt cgcggtacgg
    15961 aaagtgtagg ccttgccgcc aacagtgccg tagtagcctc atcgatcgcg gtattcatca
    16021 tcgatatgct ggcggcccag atcaccgatc tattatgaac tatataaaaa acgatacggt
    16081 ccccatccct gccgctgaca ccagcgacca gcccgacatc gacccccagg ccatgcccga
    16141 tgccagcgac cagcccgaaa tcgccacaca gcccgaaatc gccccccagc ccacacccga
    16201 cacaagcgtc cagcccgacg aaaccgctgc gctcgcttca gtcgatgaag aacccgtcgt
    16261 cctcatccgc gggctggtca agtccttcaa agaccatgag gtccttcggg gcgtcgacct
    16321 tcatgtcaaa aaaggcgaaa atctcgtcat tctcggcaaa tcggggacag gtaagtccgt
    16381 cctgatcaaa tgtctcgtgg ggttggaatg gccggatgcc ggcaccatct ccattttcgg
    16441 cgaagatatc gccgctctgt cctataataa aatgaacgca cttcgtgtac gga
//
LOCUS       DASYSV010000606         5502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_893171, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000606 DASYSV010000000
VERSION     DASYSV010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893171"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VGQ51_18940"
     CDS             complement(<1..834)
                     /locus_tag="VGQ51_18940"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV9038725.1"
                     /translation="MMGIYLTGAILIGSALFLNRHRGWNYLLVTAFVLLQWALTIHVW
                     THRDRTDLVYFTPDALAVLFLTTLSVISVPAFWHSYRYFASDKEIPRERSIYFAAMVV
                     LLAALSAAYLSNHIAITWIFVELTTLSASALIYHRRNVRTLEGTWKYVFVCSISITLV
                     FIGILLLTMGVQEAGFSDLSYTSLAARAGLFNTFWLKLAFLFIFTGFTVKAGLVPMYT
                     AGIDAKDKAPSPAGALFSSVLIMVGFLGIFRVYAIISHTEIFPWAGRILFISGFLSVF
                     VA"
     gene            complement(844..1473)
                     /locus_tag="VGQ51_18945"
     CDS             complement(844..1473)
                     /locus_tag="VGQ51_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038726.1"
                     /translation="MTNILLILFVISLVHMGLSKRLNTYTAVLAFQGILLFGVAFIEL
                     IQINVPNLLFVLLETIGFKTIAIPWFLNYVIRRNKITRESEPFLPDFISVVIVTTIII
                     GSFLLSNVIRDTHVRKILFVAALSALFTGLYFIISRKKIITHVMGFLVIENGVFVLSL
                     AVGAEMPLLVNIGILLDIFASVFLLGVFVNKIGDVLQDQDVDQLRNLKD"
     gene            complement(1486..2400)
                     /locus_tag="VGQ51_18950"
     CDS             complement(1486..2400)
                     /locus_tag="VGQ51_18950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEV9038727.1"
                     /translation="MGILNFLLIAGASGIFPGIIIRTKSRFSGRKGPGIFQPLKDVWK
                     LLRKGAVYSETTSFIFQIAPSIYFASVLMAIAMIPFGSKGGLISFPGDFVFFAYVLAL
                     GKFFTIIAALDTGSSFEGMGASREALYSLLAEPAFFILMGSFALLTGHTSFREIFGSL
                     HFGSGISYAIGLLAAFVITLTTLIENSRMPIDDPKTHLELTMVHEVMILDNSGFDLGL
                     ILQTGHLKFAMYGALIANFFLTPGSELYYSIGIFLVIEVLFAVAIGVIESFMARFRMN
                     HNPQYIFTLTSMSLLIFLGVLLLLGKIV"
     gene            complement(2405..4435)
                     /locus_tag="VGQ51_18955"
     CDS             complement(2405..4435)
                     /locus_tag="VGQ51_18955"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV9038728.1"
                     /translation="MPVAVIIATFFIASLFILLVGGRWKGFVAVAAVLVNGLVSANIA
                     IRALTGQAFEEHFYTGLYFGELAIRVDALSAWFILLTDFTLLTGILYGMRYLKPYEGK
                     AAELNVHYPCYILIHVAMLGIFTIQHLLWFLTAWEIMTLTAFLMIIFDHGKMEVVRAG
                     INFLIQAHISFTFLTVAFIWIHLQSGAYDLAGVSTYLSRAHPALGFLLFLLFFAGFGI
                     KAGFVPFHTWLPYAHPAAPAHVSGVLSGVLIKLGIFGILRIILLARGGFVLIGYTILI
                     FSIVSGVYGVVMAIVQHNLKKLLAYHSIENIGIIGMGMGVGCIGIGLKNYLLIFAGFG
                     AALLHTLNHALFKSLLFYAAGTVHQATHTMDIERLGGLVKKMPHTTVLFLAGSVAICG
                     LPPLNGFVSEFLLYSGLFHGMAANLETSTLLLLAALSALALIGGLAMLCFTKAFGIIF
                     LGRPRHLLPEPFKEDRLGLPPGYLTLALMVAIGLLPSLFLRLAAPAIALFTSVPGQLP
                     AVDLASHIPLLSTMQAISVSTVVFLLMILAILLIKRKLASPVAAWSPTWGCGYAAPTA
                     RLQYTANSFVRPLRKLIRPLLMMNKSEGVIGGVFPSDPIRSDTHPYDKLEATLIDRPL
                     RGLRRFLGRFDFLQNGSAPAYILYGMGFIVLVLLIPFLADAVTYLFNLFKQV"
     gene            complement(4438..>5502)
                     /locus_tag="VGQ51_18960"
     CDS             complement(4438..>5502)
                     /locus_tag="VGQ51_18960"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV9038729.1"
                     /translation="NIGLIGMGIGIGLIGKASGNTAIFYIGFLAALLHTLNHSLYKSL
                     LFFTAGNIYRQTHTRDMDRLGGLIRRMPQTALLFLSGSLAICALPPFNGFISEFLIYT
                     GFTEGIKTAGVQLNLLMIAGIAALAIAGGLSLFTFTKSFGVIFLGSPRTAAAERPEEV
                     PLPMRLPLYVILLIMLLIGFFPNTLIGVLLPVADVFDGGIRAGNYSPNALLGWAGPLF
                     GVLVGVVFVIYRVRTALVRKMPAPVLSTWGCGYIAPDSRMQYTGKSFSKSLAKLFSFF
                     TTESKKYAEIMGIFPEGRSYQSHYIDFFESRIFHWLRNRLLAAINSFGFIQTGQTQQY
                     VLYGLFFMVILIIASFLNYL"
BASE COUNT         1518 a         1388 c         1548 g         1048 t
ORIGIN      
        1 ggccacgaag acggaaagaa atcctgagat gaacagtatc ctgccggccc atggaaatat
       61 ctccgtatgg ctgatgatcg catagacccg gaagatgccg aggaacccga ccatgatcag
      121 tacgctgctg aacaacgcgc ccgcggggga cggggcctta tccttggcat cgatgcccgc
      181 ggtatacatc ggcaccaggc ccgcctttac cgtaaaaccg gtgaagataa aaagaaaggc
      241 cagcttgagc cagaaggtat tgaacagacc tgccctggcg gcgagggatg tatagctcag
      301 atcgctgaag ccggcctcct gtacccccat cgtcaggagc aggataccga taaagaccag
      361 ggtaatgctg atggaacaga cgaatacata tttccaggtg ccttcaagcg tgcgcacatt
      421 ccggcgatga tagatcagcg ccgaagcgct cagcgtcgtc agctccacaa agatccaggt
      481 gatggcgata tgattggaca ggtaggccgc actgagtgcc gccagcagaa cgaccattgc
      541 ggcaaagtaa atgctacgtt ctctcgggat ctctttgtcc gaggcgaaat aacgatagct
      601 atgccagaag gccggcacgc tgatcacgct cagggtcgtc aggaaaagga ccgccagcgc
      661 gtccggcgta aagtagacaa ggtctgtccg gtcccggtga gtccagacat ggatcgtcag
      721 cgcccactgc aatagcacga aagccgtgac caggagataa ttccatcccc tgtgcctgtt
      781 caggaaaagc gcggagccga tgagtatggc gccggtgaga tagattccca tcataacgat
      841 cgattagtct tttaagtttc taagctggtc gacatcctga tcctgcagca catcgccgat
      901 cttgttcacg aataccccca gcagaaatac gctggcaaaa atatccagca ggatgccgat
      961 gttcaccaac aagggcattt cagcacccac cgccagcgac aggacgaata cgccgttctc
     1021 gatgacaaga aaccccatga cgtgcgtaat gatctttttg cgggagatga tgaagtatag
     1081 acccgtgaac aaagccgaca gagccgcgac gaacaatatt tttcggacat gcgtatcccg
     1141 gatgacattg gatagcagga aggagccgat gatgatcgtc gttacgataa cgacggagat
     1201 gaaatccggc aggaatggtt ctgattcccg tgtgatcttg ttccgccgga tcacatagtt
     1261 gaggaaccag gggatggcaa tggtcttgaa cccgatggtt tccagcaata cgaaaaggag
     1321 gtttggcaca ttgatctgta tgagctcgat gaaggctacg ccgaacagga ggatcccctg
     1381 gaaagccaga acggccgtat aggtattcag ccgtttggag agtcccatgt ggaccaggct
     1441 gatgacaaag agaatgagta atatgttggt catagactgc attttttaaa cgatcttccc
     1501 caatagcagc agcacaccga ggaagatcaa taacgacatc gaagtcagcg taaatatgta
     1561 ttgcggatta tggttcatgc ggaaccgggc cataaaggac tcgatgacgc cgatcgccac
     1621 ggcgaaaagt acctcgatca ccaggaatat gccgatggag taatatagct cggaacccgg
     1681 cgttaggaag aagttcgcga tcagggcgcc atacatggca aacttcagat gcccggtctg
     1741 taagatcaga cccaggtcga atccgctgtt atccaggatc attacctcat gtaccatcgt
     1801 cagctccaga tgggttttag gatcatcgat cggcatgcgg ctgttctcga tgagggtagt
     1861 cagcgtgatc acgaaggcgg ccagtaggcc gatggcatag gagataccac tgccgaaatg
     1921 cagcgagccg aagatctccc ggaaagaagt atggccggtc agcagggcaa acgatcccat
     1981 caggatgaag aacgcgggtt ctgcgagcag tgaatagagc gcctcccggc tggcgcccat
     2041 accttcaaag ctgctgccgg tatcgagtgc ggcaataatg gtaaaaaact tgcccagggc
     2101 cagcacatag gcaaagaaca cgaaatcccc cgggaaggaa ataagtccgc ccttgctgcc
     2161 gaaggggatc atggcaatgg ccatgagcac gctggcaaaa tagatgctgg gtgcgatctg
     2221 gaagatgaag ctggtcgttt cgctgtaaac ggcgcctttg cgcagcagtt tccagacatc
     2281 tttcagcggt tgaaaaatgc ccgggccttt ccggccggaa aatcggcttt tggtgcggat
     2341 gatgatcccc gggaaaatcc cgcttgcacc tgcgatcaat aggaagttca gtatgcccat
     2401 tacgctatac ctgtttgaat aaattgaata aataagtgac ggcatctgcc aggaaaggga
     2461 tgaggaggac aaggacgata aaccccatcc cataaaggat ataggcgggg gcgcttccgt
     2521 tctggaggaa atcgaagcgc cccaggaatc gcctcagacc acgcagcggc cggtcgatca
     2581 gggtcgcttc cagtttatcg tagggatggg tatccgatcg gatagggtcg gaagggaaaa
     2641 cgcccccgat cactccttcg gatttattca tcatcagcag gggtctgatc aatttacgca
     2701 gaggcctgac gaatgaattg gcggtatatt gaagccgggc cgttggagcg gcatacccac
     2761 agccccaggt cggtgaccat gcggcaaccg gcgatgccag tttgcgtttg ataagcaata
     2821 tcgccaggat catcagcagg aagaccacgg tagagacgct gatggcctgc atggttgaca
     2881 gtaagggaat atgtgacgcc agatcgactg ccggaagctg gcccgggacg ctggtgaaca
     2941 atgcgattgc aggcgccgcc agccggagga acaaggaagg tagcaggccg atagcgacca
     3001 tcagggcaag ggtcagatag ccgggaggaa gccccagcct gtcttctttg aagggttccg
     3061 gcaggagatg gcgtggacgt ccaagaaaaa taatgccgaa cgccttggta aagcaaagca
     3121 tggccagccc cccgatcagt gccagcgcgg acagggccgc cagcagcaaa agagtagagg
     3181 tttccaggtt ggctgccatg ccatggaaca aacccgagta gaggaggaac tccgagacaa
     3241 aaccattcaa cggtggcaac ccgcaaatcg ctaccgatcc cgccaggaac aggacggtcg
     3301 tatgcggcat tttttttacc aggcctccca gccgttcgat gtccatcgta tgggtagcct
     3361 ggtggacggt ccccgcggca taaaataaaa gggatttgaa cagcgcgtga ttcagcgtgt
     3421 gtagtaaggc tgcgccgaaa ccggcaaaaa tcagcaggta gtttttcaac ccgatgccaa
     3481 tacagcctac tcccatgccc attccgatga taccgatatt ttcaatgcta tggtaggcga
     3541 gaagtttctt gaggttgtgt tggacaatgg ccatgacgac cccatagacg ccggagacga
     3601 tggaaaagat aaggatcgta taaccgatca acacaaatcc tcctctcgcc aataagatga
     3661 tgcgcagtat accgaagata cccagcttga tcaggacgcc ggacaaaacg ccggagacgt
     3721 gggcaggcgc cgcaggatgg gcatacggca gccaggtatg gaaaggcaca aaccccgcct
     3781 tgatgccgaa accggcaaaa aagaggagga ataacaggaa ccccaatgcg ggatgcgccc
     3841 gggacaggta ggtggaaacc ccggccaggt cgtaggcgcc tgattgcagg tgaatccaga
     3901 tgaacgccac cgtgaggaaa gtgaaagaga tatgagcctg gataagaaaa ttgatgcctg
     3961 ccctcaccac ttccattttt ccatgatcga agatgatcat taaaaaggca gtcaatgtca
     4021 tgatttccca ggcagtgaga aaccatagca ggtgttggat ggtgaagatc ccgagcatag
     4081 cgacatggat caggatatag cagggataat gtacattgag ttccgctgcc tttccttcat
     4141 acggcttcag ataacgcatc ccataaagga tgcccgtcaa cagggtaaaa tcggtgagca
     4201 ggatgaacca ggcggagagc gcgtccacgc ggatcgcgag ctcgccgaaa taaagtccgg
     4261 tatagaagtg ttcttcaaag gcctgccccg tcaatgcgcg gatggcgatg ttcgcactca
     4321 ccaggccgtt cacgagcaca gccgcgacgg caacgaagcc tttccaccgc cccccgacca
     4381 ggagtataaa cagggaggcg ataaagaaag tggctatgat gactgctaca ggcacatcta
     4441 taaatagttt aaaaaactgg caataataag aatgaccatg aaaaagagac catagagtac
     4501 gtactgttgc gtttgacccg tttggatgaa gccgaaggaa ttgatagcgg cgaggagcct
     4561 gttcctgagc caatggaaga tccggctttc gaagaaatca atgtagtggg actgatagct
     4621 gcgcccttcg gggaagatac ccattatttc ggcgtacttc ttactttccg tggtaaaaaa
     4681 ggaaaatagc tttgcaaggc tcttgctgaa tgacttcccg gtatactgca tccgcgagtc
     4741 aggggcgatg tagccgcatc cccaggtaga caaaacagga gcgggcatct tgcggaccag
     4801 ggcagtccgg acgcggtaga tcacgaagac cactccgacc aggaccccga agagtgggcc
     4861 tgcccaccct aacagggcgt taggggagta attccccgcg cgaatacccc catcaaagac
     4921 atctgcaacc ggcagtagca cccctataag agtattcggg aaaaatccga taaggagcat
     4981 gatcagcagg atgacataca gcggcagccg catcggcagg ggcacttcct caggcctttc
     5041 agctgccgcc gtgcgcggcg acccgagaaa aattacgccg aacgatttcg tgaaagtgaa
     5101 aagggaaaga cctcctgcta tagctaaagc ggcaataccc gcgatcatca gcaggttcaa
     5161 ttgtacaccc gccgttttta tgccttcggt aaaccccgtg tatataagaa actcggagat
     5221 aaagccattg aaagggggaa gagcgcagat agcaagagag ccggacagga agaggagtgc
     5281 cgtttgtggc atccgcctga tgaggccccc cagccggtcc atatccctgg tatgagtttg
     5341 ccggtaaata ttgcccgcgg taaaaaataa cagcgattta tacaacgaat ggttcagcgt
     5401 atgaagcaat gcggccaaaa aaccgatgta aaatatcgcg gtattgcccg atgccttccc
     5461 gatgagaccg atcccaatgc ccataccgat caggccgata tt
//
LOCUS       DASYSV010000607         6024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1920724, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000607 DASYSV010000000
VERSION     DASYSV010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920724"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VGQ51_18965"
     CDS             complement(<1..992)
                     /locus_tag="VGQ51_18965"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV9038730.1"
                     /translation="MESARKQKLAGWGNYPVAESFTLNPRSSEDARDDLKMGSLIARG
                     LGRSYADQAINENNYVAVVTKLNHFLDWNEQEGILECEAGVSLEEIISVFGPKGWLPM
                     ICPGTKFVTIGGAIANDIHGKAHHIDGSFVNCVLSFSILLADGSVVEASRTENTDLFW
                     GNFGGLGLLGVILRARIRLRKIETTYFKQKSMVIKNLDHMLEALEQYDHDYNYSVAWI
                     DALAKGSKLGSGVLTLGNSAKLSELPDKLRARPLATHKAGKLKVPFFLPSFALNGISV
                     RLLNRVIAFVQNSPKEFVHYEKFFFPLDAINDWNKGYGKRGFIQYQFVIPEDQG"
     gene            1076..2578
                     /locus_tag="VGQ51_18970"
     CDS             1076..2578
                     /locus_tag="VGQ51_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038731.1"
                     /translation="MTNLFTLRSKFFWPAVAVCLLPFVILAFLDSMAMDDYYFYDLFR
                     AQGFWGAQHELYLTWAGRYTTSFITGSFIALDLPARSPFLPTLCYFAFTWIAIGNMLR
                     SLRPLLPAGFLGRGGVWKASAVLFVLFLYVQVDIATGFYWLSSTLVYQTAFILFLLLV
                     RTILRRISLHRSFAPVDLLFLLLVVLLVGCNEIMAVFLPLFLAALAGFFYMYRRRIDR
                     WLWLGLAIAIGMGLVIFFTSGVMNYRHSLMNARTGFFSILPVIGFRTIEVLFYILKEP
                     LFWGCAFAIFLGGIAVSPQLKEGPLTIFRTRNVFLPGVVALISIVCLALAAFLLASRG
                     SIPPRALNNLGDMTAFCFLALLFLAGIHTGARWTTAILPKPSPLVQLALLVAFLTTSV
                     NYAEAWRSVGSGYLYHAVVADRDQVLKTAAAGHRHVAIVPSYDAALTEKIDRFFPHGI
                     SATAREWLLQKPTLLVVYDGAAVGDTAYAHFYGLDSVIVGPEDRRPKARH"
     gene            2761..3579
                     /locus_tag="VGQ51_18975"
     CDS             2761..3579
                     /locus_tag="VGQ51_18975"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV9038732.1"
                     /translation="MKKYFLTAIVFLVTTAAFAQKKHSKYPQNPPPAKDTVIPQQTPA
                     PAPAPTPGKNGRDWSKVNLSKRSADHFMFELGYDNWLSTPDSVNIKGFNHSVNFYFMY
                     DWPFKTDARFSVAAGLGIGSSNIYFDNTYPQVAAYNNQTLAFTQTGGGNHFKRFKLTT
                     VYLEIPVELRFALDPEHMDKSWKFALGAKIGLMVNAYTKGVDAVNSVGQTIGNITEKE
                     YGKQFFNTPKLAGTVRINKGVIGVFGQLQVNSLIKPTAGQQVFPFSFGIVLSGL"
     gene            3704..4894
                     /gene="hflX"
                     /locus_tag="VGQ51_18980"
     CDS             3704..4894
                     /gene="hflX"
                     /locus_tag="VGQ51_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV9038733.1"
                     /translation="MIEKKNSIQKAERAVLVGLVHKTQTEEQSQEYLDELAFLAETAG
                     AVTLKRFTQKLLHPDSKTFVGKGKLEEIKNYIALKDIEVVIFDDELSGSQISNIEKAL
                     DTKTIDRSDLILDIFAARAKTAQAKLQVELAQYQYLLPRLRGMWKHLERLGGGIGTRG
                     PGETEIETDRRIVKDKIALLRRRLQEIDKQAFTQRKDRGEFIRVALVGYTNVGKSTLM
                     NLLSKSEVFAENKLFATLDTTTRKVVFENTPFLLSDTVGFIRKLPHHLVESFKSTLDE
                     VREADILLHVVDVSHPGYEEQLGVVNKTLQELNAFEKPILVVFNKIDRYEELTFDAWL
                     EEGVKREIMNELRERWEHETNGHCVFISATERRNIDELRRTILEKVRELYRVRYPYKS
                     EFNY"
     gene            4902..5258
                     /locus_tag="VGQ51_18985"
     CDS             4902..5258
                     /locus_tag="VGQ51_18985"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV9038734.1"
                     /translation="MSLLKKFNWHKVADSVDAIQCNAGGIAEVSVEGKEICVGQFNGE
                     WYGFAATCPHAGGAMLDGYIDGACRVVCTIHQLRFNLKTGHDSNGEGYKLKTYPVEVR
                     EDGVYVGVEEARFKLF"
     gene            5360..5433
                     /locus_tag="VGQ51_18990"
     tRNA            5360..5433
                     /locus_tag="VGQ51_18990"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5394..5396,aa:Asn,seq:gtt)
BASE COUNT         1410 a         1497 c         1557 g         1560 t
ORIGIN      
        1 ttgccctggt cttccgggat cacgaattga tattgtatga accctcgttt gccataaccc
       61 ttgttccagt cgttgatggc atccagcggg aaaaagaatt tctcatagtg cacgaattcc
      121 ttcggggagt tttgaacgaa ggcgatgacc ctgttcaaca gccggacgct gataccattg
      181 agtgcaaagg aaggcaggaa aaatggcacc ttcagctttc cggctttatg cgtggccaac
      241 ggccttgccc taagcttgtc cggcaattcg cttaatttgg ccgagttgcc cagggtgaga
      301 acgccgctac caagcttgct gcctttggcc agcgcgtcta tccatgcgac ggaataattg
      361 taatcgtgat cgtattgttc cagcgcctcc agcatatgat ccaggttttt gatcaccatg
      421 gatttctgct tgaaataggt tgtttctatc ttcctcagcc tgatacgggc cctgaggatc
      481 acgcccagca atcccagtcc cccgaaattc ccccagaaaa gatcggtatt ttctgtccgc
      541 gaggcttcga cgaccgaccc atccgccagc aggatcgaaa aagatagtac gcaattgacg
      601 aacgatccgt cgatatgatg cgccttacca tggatatcgt tggcgatcgc tcctccgatg
      661 gtaacgaatt tcgttcccgg gcagatcatc ggcagccatc ccttcggacc gaatacagat
      721 atgatctcct cgagactgac gccggcctcg cattcgagaa tgccttcctg ctcattccaa
      781 tccaaaaaat ggttgagttt cgtaacgaca gcgacatagt tgttctcgtt gatcgcctgg
      841 tcggcatagc tacggccgag accacgcgcg atgagcgaac ccatcttcag atcgtccctc
      901 gcgtcctcag agcttctggg attcaatgta aatgattcag ctacggggta attaccccat
      961 cctgccaatt tttgcttccg ggcggattcc atcgagttta attttctccg cgaaattaat
     1021 aaaagccgcc caatattttg cctactttta ggaccattga acacataacc agctgatgac
     1081 caatttattt acgcttcgat caaaattctt ttggccggca gtggcggtct gcctccttcc
     1141 tttcgtcatc ctggcttttc tcgatagcat ggcgatggat gactattatt tttatgatct
     1201 tttccgggcg caggggtttt ggggtgccca gcatgaactc tatctcacct gggctggtcg
     1261 ttataccacc agttttatca ccggcagctt tatcgccctc gatctgcctg cccgatctcc
     1321 tttcctgcct acgctgtgct atttcgcatt tacatggatc gccatcggga atatgttgcg
     1381 gtcgctgcgg ccacttctgc cggctggttt tttgggtcgt ggtggggtat ggaaggcttc
     1441 cgctgttttg tttgtccttt ttctatatgt tcaggtggat atcgcgaccg gtttttactg
     1501 gcttagctcg acgttggttt atcagacggc attcatcctg ttcctgctgt tggtgaggac
     1561 gatccttcga aggatcagcc tccaccgctc ctttgcgccc gtagatctac tgttccttct
     1621 gctggtcgtt ttgctcgtag gatgcaatga gatcatggcg gtgttcctgc cgctattcct
     1681 ggcagcattg gcaggcttct tttatatgta tcggcggcgc atcgatcgct ggctatggtt
     1741 gggtctggcg atcgctatcg ggatggggtt ggtcatcttc tttacttcgg gggtcatgaa
     1801 ttatcgacac agcctgatga atgcccgtac cggttttttc agtatcctgc cggtcatagg
     1861 cttccgaacg atagaagtgt tgttctatat cctcaaagaa ccgcttttct ggggatgcgc
     1921 tttcgcgatc tttctcggcg gtatcgcggt ttctccgcaa ttgaaggagg gcccattgac
     1981 cattttccgg acaaggaatg tgtttcttcc gggggtcgta gcgctgatat cgatcgtctg
     2041 tttggcgctt gcggcttttc ttttggcctc gcgggggtcg atacctccac gggcattgaa
     2101 caatcttggt gacatgaccg ccttttgttt cctggcgcta ttatttttgg cgggcatcca
     2161 taccggcgcc cggtggacga cggccattct cccgaagcca tcccctttgg tacagctggc
     2221 cttgctcgtt gcctttctga cgaccagcgt caactatgcc gaggcgtggc gcagcgtggg
     2281 ttccggatat ctgtatcacg cggtcgtggc cgatagggat caggttttga agacagccgc
     2341 cgccgggcac cggcatgtgg ccatcgttcc ctcttacgat gccgcattga cagagaagat
     2401 cgatcggttc ttcccgcacg ggatcagcgc cactgcccgc gaatggttgc ttcaaaaacc
     2461 aacgctgctg gtcgtgtatg acggagcagc agttggcgat accgcctatg cgcattttta
     2521 cggtttggat tcggtaattg tcgggccgga ggaccgacgg ccgaaggcca gacattagcg
     2581 ctttgcggaa gatgcgctat ttttccagat ggcgcatctt ccgcacatat attgtgaagg
     2641 ataagtaata tcttgcggcc tgtctaaaaa gcaggccagg gcttctttaa ccttttatta
     2701 accccaagga gaatacattt gtcaaccaga acgaaaagaa caacatccat aagtgcattt
     2761 atgaagaaat atttcttaac cgctatagtt ttcttagtca caactgctgc gttcgcccaa
     2821 aagaagcata gcaaataccc tcagaatccg cctcccgcaa aagacaccgt aatcccccaa
     2881 caaacacctg cccctgctcc cgcccccact ccaggcaaaa acggaagaga ttggagtaag
     2941 gtcaacctct ccaaaaggtc cgccgaccat ttcatgttcg agctgggtta cgataactgg
     3001 ctctcgacac cggatagcgt caatatcaag gggttcaatc atagcgtcaa cttttacttc
     3061 atgtacgact ggccgttcaa aacggacgcc cggttcagcg ttgccgccgg tcttggcatc
     3121 ggtagcagca atatctattt cgataatacc tatccgcagg tagcagccta taataaccag
     3181 acgctggcgt ttacccaaac cgggggaggt aatcacttca aacggtttaa gcttacgacc
     3241 gtctacctcg agatccctgt cgaattgcgg tttgcgctcg acccggaaca catggacaag
     3301 agttggaaat ttgccctcgg cgccaagatc gggttgatgg tgaacgccta taccaagggt
     3361 gtggatgcag tcaatagcgt cggccaaacc atcggaaata tcacggagaa agaatacggc
     3421 aagcaattct tcaatacgcc aaagctggcg ggcacagtca ggatcaacaa aggggtcatc
     3481 ggcgtcttcg gccagttgca ggtcaatagt ctgatcaagc ctacggccgg acagcaggtt
     3541 tttcccttct ctttcgggat tgtcctcagc ggactgtaag gtagggcgcc gggacgcgga
     3601 actccgggcc tgaagccatg atccttcttt tggcgcctcc tacgcagttt gcacaactcc
     3661 ggccaatccg acgtaccttt gccgtccaaa ttgtcagaaa gcattgatag agaagaaaaa
     3721 cagcattcaa aaggcggaaa gagcggtcct ggtcggcttg gtgcataaga cccaaacgga
     3781 ggagcagtca caggaatacc tggatgagct ggctttttta gccgagacag ccggggcggt
     3841 gacattgaaa cgatttaccc agaaactcct gcatcccgac agcaagacct ttgtcggaaa
     3901 agggaagctg gaggagataa agaactacat cgccctcaag gatatcgagg tcgttatctt
     3961 cgacgatgaa ctatcgggct cccagatctc gaatatcgaa aaagcgctgg acacgaaaac
     4021 gatcgaccga tcggatctga tcctcgacat tttcgccgcc cgggccaaaa cggctcaagc
     4081 caagctgcaa gtggagctag ctcagtatca atatcttcta ccacgacttc gcgggatgtg
     4141 gaaacacctc gaacgtttgg gcggcggtat cggtacccgt ggtccggggg agacggaaat
     4201 agagacggac cggcgtatcg ttaaggataa gatcgcattg ttgcgccgcc ggctgcagga
     4261 gatcgataaa caggccttca cccaacgcaa ggacagggga gaattcatcc gggtggcgct
     4321 ggtgggatac accaatgtcg gaaaatctac cctgatgaat ctgctgagta aaagcgaggt
     4381 ctttgccgaa aacaagcttt ttgccacgct cgacaccacg acgcgaaagg tggttttcga
     4441 gaatacgccg ttcctgctga gcgacactgt cggtttcatc cgcaaattgc cgcatcacct
     4501 ggtcgaaagc ttcaaaagca ccctcgatga ggtgcgcgaa gcggatatcc ttttgcatgt
     4561 cgtggatgtc tcgcatcccg gctatgaaga gcagctgggt gtcgtcaaca agacgctcca
     4621 ggagctgaat gctttcgaaa agcccatcct ggtcgttttc aataagatcg accggtatga
     4681 ggaactgacc tttgatgcct ggctggagga gggggtcaag cgtgagatca tgaatgagct
     4741 ccgcgagcgc tgggagcatg agacgaatgg gcattgtgtc tttatttcag ccaccgagcg
     4801 ccgtaatatc gatgaattgc gacggacgat cctggagaag gtcagagaat tgtatcgcgt
     4861 ccggtatccg tataaatccg aattcaatta ctgatgagcc tatgagcctt ctcaaaaaat
     4921 tcaattggca taaggtcgcg gattctgtcg atgccattca atgtaatgcc ggcggcatcg
     4981 cggaggtatc agtagaggga aaggagattt gcgtggggca gttcaatggg gaatggtatg
     5041 gctttgccgc gacctgtccc catgcgggtg gcgcaatgct cgacggatat atcgatggcg
     5101 cctgccgggt cgtctgtacg atccaccagc tgcggttcaa tctcaagacg gggcatgatt
     5161 ccaacggcga agggtataag ttgaagacct atccggtgga ggttcgtgaa gatggggtgt
     5221 atgtgggggt cgaagaggca aggtttaagt tgttttagcc atacgggatt tttttgtggc
     5281 caccagcttt ttagaagggg attttcccta tttttgcaac cccttttggg gccggccgga
     5341 tcgccgtccg ggaacatatt cctccttagc tcagttggtt agagcatctg actgttaatc
     5401 agagggtcgt tggttcaagt ccaacagggg gagctagata tcaatcgttt agaaggccgg
     5461 ttttccggcc tttttcgttt ttgccagtaa tttgccagta acttgtggca gtgaagcaaa
     5521 gagctgtttt tcaggttaaa tttacaatat tcaatcctta gcatcaaata agtagtcgcc
     5581 gccactcttt gctatcattc tgttacggaa tgggcgcgga tattattgcc aaccataggt
     5641 ggacattcca gcctaccaca aagcaaaaat atcttgcatg attcgttttt aggcatcgac
     5701 aaggcattta caattgaact gataaatttt cggctgaccc caaaatgagt aaaagagcaa
     5761 gtgatcttgt tattaaattg gtatttgtta tatttagcca ttctcagcaa ttttaagtac
     5821 tttttatata ctaagcataa tctaaattga tggcgcgctc agacttatta attaaattgg
     5881 taaaagccgg aggtgctggg gatatcgtct tctttaaaaa gattgtacaa tccttaattg
     5941 cagaggagaa aagtaaacaa cactatgtat tagcgaacca gctggaaaag gaattaatga
     6001 cattaacgaa gggtgaggtt acag
//
LOCUS       DASYSV010000608         6849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1531030, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000608 DASYSV010000000
VERSION     DASYSV010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531030"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGQ51_18995"
     CDS             <1..241
                     /locus_tag="VGQ51_18995"
                     /inference="COORDINATES: protein motif:HMM:NF028498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HEV9038735.1"
                     /translation="AYYINAHAAKFVMPGSSRIESNSDDSLPNVAFRTPHGKAVLIVL
                     NNTNSAKTFTISFRGRSISPKLNAGATATYFFGID"
     gene            complement(242..2011)
                     /locus_tag="VGQ51_19000"
     CDS             complement(242..2011)
                     /locus_tag="VGQ51_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEV9038736.1"
                     /translation="MRHSFRLLCVIVLTTGLAPAIAQVSPDAYVPCQEMPHLIENYNA
                     DAQAITRFYTSSFFGGRNGGGAMTGGSPDKRARLDALNHEYLEKLDRLDFKGLPQECK
                     VDYILFKRDLKERLYQSAAEAAQYEKLRAWFPFADSIYAVERLRRRGHVLNSERLAKD
                     WFDDTRTIMRLRGRLKNDTSLDMSAIYEARLVVADLKRATASIYEFYNGYDPLFTWWM
                     PKTYKELDSALTGYAAAFSERSKRFAPHDESGIVGRPVGRAELVRQLQFEMIPYTPEE
                     LIDIANREFAWCDREMLKASHEMGLGDDWKAALEKVKNSYVPPGQQPEMILRLFNESV
                     DFLKKHDLVTIPPLAEETWGMIMMTPERQLVNPFFTGGNEISISYPTNTMEEDDRLMS
                     MRGNNPYLSRATVHHELIAGHNLQAYMNARYRTYRNFGSGFWTEGWSLYWELLLWDLK
                     FPQTPEDRVGMLFWHMHRCARIIFSLNYHLGKWTPEQCIDFLVDRVGHERANAAGEVR
                     RSFETNYPPLYQLAYLTGGRQFYALKKELVDGGKMTYRQYHDAVLHLGAMPVEMIRAI
                     LTKQPLEKDYTAKWRFYDESGVN"
     gene            complement(2017..4710)
                     /locus_tag="VGQ51_19005"
     CDS             complement(2017..4710)
                     /locus_tag="VGQ51_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEV9038737.1"
                     /translation="MQKRCSLPLLMGFVFLFHFASAQTTPTAGNLAIVATTTGSGRPG
                     AILTTPNDGLTPTPPGNLRRPAPPRQADQWIEYDWKSPVTTRRVEVFWWDYGNPARLP
                     GGYHFEYWDGSAFRAVSHVSGGGETNGAFNASDFDEVTTTRLRLVMDSADRYATVITE
                     WKVDESGNSKPVAPLVNAGADRDVMLDGKTYLDAHVRSVHPVTKTTWTKIDGPGEVHF
                     ANEADAATTASFTTRGVYRLSFTAWDGGEQSSGTISVKVVDPPPARRLDVVYTKRYSV
                     DSRLWNDRFKAIIVHWIPYCVEQIERTDLSTGQGGLDNFIEAAKALRGEPHGKHKGYV
                     FSNAWVYQTVEAMCEALMVDPKGDTAVIAAQEKMKTTLAKWIPIILAAQEPDGYLQTA
                     WTLRDTSRWHERWSPESRGNHEGYTAGYFIESAINHYTLTEGKDLRLYNAAKKLANCW
                     VAHIAPGKIAWFDGHQEMEQALVRFGRFVNDMEGHGHGDSYIRLAKFLLDNRHGGSDY
                     DQSEVPVQEQYEAVGHAVRATYTYSGMADVAAEMGDVDYQSAVLSLWDNIVNRKYYIT
                     GGIGSGETSEGFGPDYSLRNNAYCESCSSCGLIFFQYKMNLAYHDAKYADLYEETMYN
                     ALLGSLSLDGRDFLYTNPLSSSEARYAWHVCPCCVGNIPRTLLMMPTWTYVRGDDGLY
                     VNLFVGSRMRVEKVGGADKTNVEMVQETDYPWSGKVAITVNPGKTTEFTVYVRVPNRT
                     TSQLYTPQPAVSGLLSLSVNGQTVTPLIEHGYAVIRRTWKKGDKIAFELPMEIQRVTA
                     DSKVAADRGRVALRYGPLLYNVETADHQDVTKSIGSGPLRLVWKGDLLHGVMAIEGSW
                     ADGTPLLAIPDYARLNRVPVTAVPDDSERAQPPVSIIWIKQ"
     gene            4801..5568
                     /locus_tag="VGQ51_19010"
     CDS             4801..5568
                     /locus_tag="VGQ51_19010"
                     /inference="COORDINATES: protein motif:HMM:NF026098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV9038738.1"
                     /translation="MRIGFIGVGNIAAAVVEGLCTSGYAGLEIFLSPRNMTRSASLAA
                     TYSNVRRLGSNQEVLDQSEIVFISVRPAIAQSVLKDLSFRQDHIVVSLVALLNYAELQ
                     ELVRPAAQVCRAIPLPTVVRHNCPIPLFHATATVVQLFGYLGEPIPVHDEEQLHALWS
                     LTGLISPYYELLASLSEWTMTHGVAPAAANAYVANLFQSLSYLARHADAIDFREMAKH
                     AMTPGGINEQASRGIRENGAHNAWVTAVDGVLLRFQF"
     gene            5590..>6849
                     /locus_tag="VGQ51_19015"
     CDS             5590..>6849
                     /locus_tag="VGQ51_19015"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038739.1"
                     /translation="MFRNYFKIAWRNLWKNKVYSAINILGLAIGMAACIVILLFVSYE
                     RGFDRMHTKNIYRLNEVQDWPGMLAAQKVALTMYPMAKTMKNKFPQILQATHVRWYEK
                     YEITNKDQRLFLPQVLAVDTPFLHMFDFPLLKGDRATALRDPHSVVLTETTARKLFGS
                     ADPMGKTISHYANDTVLFKVTGVLADVPENSQFQFDALASFNTWIRPRWDSAWGGNWL
                     NTYFELAPGTNTAALEKKFPSYIREHLGEDGVKGYQLFLLPLRAVHAHSADVGLDYIN
                     YQKFDSSYTNIFTVIALIVLAIACINFMNLSTARSAERAREVGIRKSIGAMQTQLSVQ
                     FLSETILLALLALVLATGLVAAVLPGIDSLSQRHLEIVFNQHVWIIPLVVLGTIGVGF
                     LSGLYPAAYLSSFQPAKVLKGLPQLGKN"
BASE COUNT         1427 a         1985 c         1872 g         1565 t
ORIGIN      
        1 ggcctattat atcaacgcgc atgcggcaaa attcgtgatg ccggggtcgt cgcgtatcga
       61 aagcaattcc gacgactcgt tgcccaatgt agcatttcga actccgcatg gaaaggcggt
      121 gctgatcgtg ctgaacaata caaattcggc caaaacattt acgatcagct tcaggggacg
      181 atccatcagc ccgaaactca atgccggcgc aaccgccacc tatttcttcg gtatcgatta
      241 gtcagttcac cccggattcg tcatagaacc gccatttggc ggtataatcc ttttccagcg
      301 gctgtttcgt caatatcgcc cggatcatct cgacgggcat ggcgcccaga tgcagcaccg
      361 catcatgata ttgcctgtag gtcattttgc cgccgtccac cagctctttc ttcagcgcat
      421 agaactgtct tccgccggtg agataagcca gctggtatag aggcgggtaa ttggtttcga
      481 atgaccggcg cacctctccg gcggcgttgg cccgttcgtg tcccacgcgg tccaccagga
      541 agtcgataca ttgctccggc gtccatttac ccaaatgata gttgagcgaa aagatgatcc
      601 gtgcgcagcg atgcatatgc cagaacagca tcccgacgcg gtcttcgggg gtctgcggga
      661 atttcaggtc ccacaacagc agctcccagt agagcgacca gccttcggtc cagaacccgg
      721 agccgaaatt gcggtaggta cggtaacggg cattcatata cgcctgcagg ttgtgaccgg
      781 cgatcagctc atgatgtacg gtcgcccggg aaagataagg attgttgccg cgcatgctca
      841 tcagacgatc gtcttcttcc atagtattgg tggggtagga gatgctgatc tcattgcccc
      901 cggtaaagaa aggatttacg agctgccgct cgggcgtcat catgatcata ccccaggtct
      961 cttcggcgag cggcgggatg gtcacgaggt catgcttttt caaaaagtcc accgattcat
     1021 tgaagagacg caggatcatt tcgggctgct gaccgggagg tacatagcta ttcttcacct
     1081 tttccagcgc ggccttccag tcatccccta aacccatttc gtgcgacgcc ttgagcattt
     1141 ccctgtcgca ccaggcgaac tcgcggttgg cgatatcgat cagctcttcc ggcgtgtagg
     1201 ggatcatctc gaattgcagc tgtcgcacca gctccgcgcg gccgacagga cgaccgacga
     1261 tcccgctctc atcgtgcggg gcgaaccgct tgctcctttc cgaaaaggcg gccgcataac
     1321 cggtcagcgc ggaatccagc tctttatagg tcttcggcat ccaccaggtg aataagggat
     1381 catacccgtt gtagaattca tagatgctgg cggtggccct tttcagatcg gcaacgacca
     1441 gccttgcctc ataaatcgcc gacatatcca gagacgtgtc gttcttcagc ctgccccgca
     1501 gacgcatgat cgtcctggta tcgtcgaacc agtctttggc gagcctttcg gaattgagga
     1561 cgtgcccccg ccgccgcagc cgctcgacgg catagataga atccgcgaaa gggaaccagg
     1621 ccctgagctt ttcatactgt gccgcttccg cggccgactg atataaccgt tccttcagat
     1681 cgcgtttgaa caggatgtaa tcgaccttgc attcctgcgg aagacccttg aaatcgagac
     1741 ggtccagctt ttcgagatat tcgtggttca gcgcgtcgag ccgggctcgt ttgtccggcg
     1801 acccgccggt catggcgccg cctccgttgc ggccgccaaa aaagctgctc gtataaaaac
     1861 gcgtgatcgc ctgcgcatcc gcattgtagt tttcgatcag gtggggcatt tcctggcagg
     1921 gcacataggc atcgggcgaa acctgtgcta ttgcaggtgc cagtcctgtc gtcaatacta
     1981 tgacgcagag caaccggaat gaatggcgca tgatcgctat tgttttatcc agatgatgga
     2041 aacgggtggc tgcgctcttt cggaatcatc gggtaccgcc gtaacgggta cccggttcag
     2101 ccgcgcatag tcgggtatcg ccagaagagg agtaccgtcc gcccagctgc cttcgatggc
     2161 catgaccccg tgcaggagat cgcccttcca aaccagccgc aagggtcccg atccgatgga
     2221 cttcgtgaca tcctggtgat cggccgtttc gacattataa agcaagggcc cgtacctcaa
     2281 tgccacacgc cccctgtccg ccgccacctt actatctgcc gttacccgtt gtatttccat
     2341 cggcaattcg aaggcgatct tatcgccttt tttccaggtc cgccggatga cggcataacc
     2401 gtgttcgata aggggcgtga ccgtctgtcc gttcacggac aacgacaata aaccgctgac
     2461 ggcgggctga ggcgtataga gctggctggt cgtacgattg gggacgcgca cgtatactgt
     2521 gaattccgtt gtctttcccg gattcaccgt tattgccacc ttcccgctcc acgggtagtc
     2581 cgtctcctgc accatttcca cattcgtctt atcggccccg cccaccttct ccaccctcat
     2641 cctgcttccc acgaagaggt tcacatagag accgtcgtcc ccgcggacat aggtccaggt
     2701 cggcatcatg agcaatgtac gcgggatatt gcctacgcaa cacggacaga catgccaggc
     2761 atagcgcgct tccgaggaag acaaggggtt ggtgtacaag aaatccctgc cgtccagcga
     2821 caatgatccg agcaatgcgt tgtacatcgt ttcctcatac agatcggcgt attttgcatc
     2881 gtggtaggcg aggttcattt tatactggaa aaagatgaga ccgcagctgc tgcaggattc
     2941 gcaataggcg ttgttgcgaa gcgaatagtc gggcccgaac ccttcggagg tctcgccgct
     3001 gccgatgccg ccggtgatat aatatttccg gttgacgata ttgtcccaaa gcgacaggac
     3061 cgcgctttgg taatccacgt cacccatctc ggcggccacg tcggccatac cggaataggt
     3121 ataggtggcc cggacggcat gtccgacggc ttcatattgc tcctgcaccg ggacctcgct
     3181 ctggtcgtaa tcgctgccgc cgtggcgatt gtcgagcagg aatttggcga gtcggatgta
     3241 gctgtcgcca tgtccatgcc cttccatatc gttgacaaaa cgcccaaaac ggactaaggc
     3301 ctgttccatc tcctggtgtc cgtcgaacca ggcgatcttt cccggggcaa tatgtgcaac
     3361 ccagcaattg gccagcttct tcgccgcgtt atatagcctc agatctttcc cttcggtgag
     3421 agtataatga ttgatcgccg actcgataaa ataacctgcg gtatatcctt cgtggttgcc
     3481 gcggctttcg ggcgaccagc gttcatgcca gcggctggtg tcgcgcaggg tccaggccgt
     3541 ttgcagatag ccatcgggtt cctgcgccgc gaggatgatc gggatccatt ttgcgagtgt
     3601 ggtcttcatc ttttcctgcg cggcgataac ggccgtatct cctttcggat cgaccatcag
     3661 ggcctcacac atggcttcca ccgtttgata gacccatgca ttggaaaaca cgtaaccttt
     3721 gtgtttcccg tgcggttcac cccggagcgc tttagcggct tcgataaaat tatccagccc
     3781 gccctgcccg gtggacagat cggtgcgctc gatctgttcg acgcaatagg gtatccagtg
     3841 gacgatgatg gccttgaaac gatcgttcca caaccggctg tcgacgctgt atcttttggt
     3901 ataaaccacg tccagccggc gggccggagg gggatcgacg acctttacgc tgatggtacc
     3961 gctgctttgc tcgccgccat cccaggccgt aaaggataac ctgtataccc cgcgggtcgt
     4021 aaaactcgcg gtggtcgcgg catcggcctc gttggcgaaa tgtacctcac cgggtccgtc
     4081 gatcttcgtc cacgtggtct tagtcacggg gtgtacactc cggacatggg cgtcgagata
     4141 tgtttttccg tccagcatca cgtcccggtc ggcgccggca ttgaccagcg gcgcgacagg
     4201 tttcgagttt ccggattcat ctaccttcca ttccgtgatc acggtcgcat accggtcggc
     4261 cgaatccatc accagccgga gacgtgtggt ggtcacttcg tcgaaatccg acgcattgaa
     4321 ggctccgttg gtctcgccac cgcccgagac atgacttacc gcgcgaaaag cggagccgtc
     4381 ccagtattcg aaatgatagc ctcccggcag ccgcgccgga ttgccatagt cccaccaaaa
     4441 gacctcgacc cgcctggtcg ttactggtga tttccaatca tattctatcc actgatcggc
     4501 ctgccgcggc ggagccgggc gtctcagatt gcccggcggg gtaggggtca gcccatcatt
     4561 tggcgtggtt agaatggctc cgggtctgcc tgaaccggtg gtggtggcga caatggccag
     4621 attaccggcg gtcggcgtgg tctgtgccga tgcgaaatga aacagaaaga caaatcccat
     4681 aaggaggggg agggaacaac gcttttgcat gattaaatat acgccatgac ggtcaatcct
     4741 gcttttccat aattctcccg tgttggtccg attttgaccc gatgcgcgta tatttgattt
     4801 atgcgaatcg gatttatcgg agtcggcaac atcgcagctg ctgttgtcga agggctatgt
     4861 acttcgggct atgccggact ggagatcttt ttgtccccgc gtaacatgac caggtctgca
     4921 tctctggccg ccacatattc caatgtaagg aggctcggaa gcaaccagga agtgctcgat
     4981 caaagcgaga tcgtttttat ttcggtgcgg ccggccatcg cacaaagcgt cctgaaggac
     5041 ttatccttcc ggcaggatca tatcgtcgtt tcgctggtgg ccctgctgaa ttatgccgag
     5101 ctgcaggaac tcgtccggcc ggcggcgcag gtgtgccgcg cgataccgct gcctaccgtc
     5161 gtccgccaca attgtcccat tcccttattc catgctaccg cgacggtcgt ccagcttttt
     5221 ggatacctgg gcgagcccat acccgtacat gacgaagaac agctgcacgc gctgtggtcg
     5281 ctgaccggcc tgatctcgcc ctattacgag ctgctggcga gtctcagcga atggacgatg
     5341 acgcacggcg tagccccggc cgccgcgaat gcttatgttg cgaatctttt tcagtcactg
     5401 tcctatttag cgcgacacgc cgacgctatc gatttccggg agatggccaa acatgccatg
     5461 acaccgggag gcatcaatga acaggcatcg agggggatca gggagaacgg agcgcacaat
     5521 gcctgggtga ccgccgtgga tggcgtgctg ttgcggtttc agttttgaga tcaccttaaa
     5581 ataaacacca tgttccgcaa ctacttcaag atcgcctggc gcaatctgtg gaagaacaaa
     5641 gtctattccg cgatcaatat actgggactg gccattggca tggccgcctg catcgtcatc
     5701 ctgcttttcg tgtcctacga acgcggcttc gaccggatgc atacgaaaaa catctaccgg
     5761 ctgaatgaag tacaggactg gccgggaatg ctcgccgcac aaaaggtcgc cctgacgatg
     5821 tatcccatgg cgaagaccat gaaaaacaag ttcccccaga tcctgcaggc cacacacgtt
     5881 cgctggtatg aaaaatatga gatcaccaac aaggaccagc ggctctttct gccgcaggta
     5941 ttggcggtgg acacgccatt cttacacatg ttcgattttc ccctgttgaa gggcgacagg
     6001 gccaccgccc ttcgggaccc acacagcgtg gtccttacgg aaaccactgc gcgcaagctc
     6061 ttcggcagtg ccgatcccat gggtaagacg atctcccatt atgccaacga tacagtgctc
     6121 ttcaaggtca cgggcgtgct ggccgatgtc cccgagaact cgcaattcca attcgatgct
     6181 ctggcatctt tcaacacctg gatcagaccg cgatgggata gcgcctgggg cggaaattgg
     6241 ctgaatacgt atttcgagct tgcgccgggt accaatacgg ccgccctcga aaagaaattc
     6301 ccttcgtata tcagggagca tctcggcgaa gatggcgtta aagggtacca actcttcctg
     6361 ctgccgttac gcgccgtgca tgcgcattcg gccgatgtcg ggctcgatta tatcaactac
     6421 caaaaattcg acagcagcta taccaatata ttcaccgtga tcgcactcat cgtactggcg
     6481 atcgcgtgta tcaatttcat gaatctttct acggcccgtt cggctgagcg ggcgagggaa
     6541 gtgggcatcc gcaaatcgat cggcgccatg cagacgcagc tcagcgtaca attcctcagt
     6601 gaaacgatat tgctggcact actcgcgctg gtcctggcaa cgggtctggt agcagctgtg
     6661 ctgccgggta tcgacagtct gagccagcgg catctggaga tcgtattcaa tcagcatgtc
     6721 tggataatcc cgctggtggt gttagggacc atcggcgtcg gctttctgag cggactgtat
     6781 cccgcggcct atctatcctc cttccagccg gctaaggtgc tgaaggggtt gccgcagctc
     6841 ggaaagaac
//
LOCUS       DASYSV010000609        10638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_248463, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000609 DASYSV010000000
VERSION     DASYSV010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248463"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="VGQ51_19020"
     CDS             complement(<1..1642)
                     /locus_tag="VGQ51_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614194.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEV9038740.1"
                     /translation="MNSNLVFSDKLVFLIYFLIVAFYGIWIYRRKRKATTDTKDFFLA
                     EGSLTWWAIGASLIASNISAEQFVGMSGDGFFAGIAVASYEWIAAVALIIIAVWFMPV
                     YLKNKIFTMPQFLKMRYNETVSLIMAIFWLLLYIFVNLTSILYLGAVAINGLLGGEYL
                     HTVMIVLAIMALLITLGGMKVIGYTDVIQVSVLIIGGLATSYLALTVVAKSFGLGDSA
                     WAGFTAMMKDAPEHFRMIFHKPTATSSQLDINKYLILPGLAMYAAGQWIVNLNYWGCN
                     QYITQRALGANLQTARTGILFAGFMKLLMPVIVMLPGIAAFVLYKHGHLNLEGGSKDY
                     AYSAILGFLPAGLKGLAIAALTAAIVSSLAGKVNSISTIYTLDIHKKYFDKAADEQKM
                     VWTGRIVVVVAMIVGIIFTWTDVLGISGKGGFTFIQQYTGFISPGVFAMFLFGMFWKR
                     TTGTAAIAGLIVGFVLCVFFNTNAPEVLGHNTWLYTAYPTQIEQNGALVTVWQIPFQV
                     CMGLAFLFTALVMIAFSLAGPKINPKAFQIDKSMFKLQP"
     gene            complement(1724..3217)
                     /gene="araA"
                     /locus_tag="VGQ51_19025"
     CDS             complement(1724..3217)
                     /gene="araA"
                     /locus_tag="VGQ51_19025"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEV9038741.1"
                     /translation="MDLKKLEVWFVTGSQHLYGEETLKQVAEHSKIIAKALNDSPDIP
                     VKVVFKPTVKSPEEIYHTCVEANNTATCIGIIAWMHTFSPAKMWIGGLRILQKPLLHL
                     HTQFNRDIPWDAIDMDFMNLNQSAHGDREFGFIMTRMRLERKVVTGHWQSPETLDRIN
                     AWTRAAAGWHDWQGARFVRFGDNMRQVAVTEGDKVEAELKFGYSVNTHGVGDLVATIA
                     SISDKAIDTLIDEYTEKYSLADSLRKGKTQHTSLREAARIELGLKTFLAEGHYKGFTD
                     TFEDLHGMVQLPGIAAQRLMAAGYGFGGEGDWKTAALVRAMKTMGTGLPGGNSFMEDY
                     TYHFEPGNNLVLGAHMLEICSSIADGTVSCEIHPLGIGGKADPVRLVFNSAAGPAINA
                     SIVDMGNRFRLLVNEVEAVAPTHNLPRLPVARVLWKPYPDMQTGCTAWIQAGGAHHTC
                     YSQNLTAEHMRDFADMAGIEFVLIGKKTDLHQLRNELRWNEAYYHLK"
     gene            complement(3234..3938)
                     /locus_tag="VGQ51_19030"
     CDS             complement(3234..3938)
                     /locus_tag="VGQ51_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV9038742.1"
                     /translation="MSKSKYSHIQEEAYEANMQLPKLGLVLFTFGNVSAADRGLGVFA
                     IKPSGVPYDDLTPEKMVIVDFEGKTVKGKLRPSSDTLTHAVLYKHWETIGGIAHTHST
                     YATAWAQAGRDIPIFGTTHADHNTVDIPCAPPMHDEMILGHYEYQTGFQIMNAFRDKA
                     LRYQEVEMVLVGNHAPFTWGRTAEKAVYNSAVLETVARMAYLTEQIRADAPRLKDSLI
                     KKHYERKHGPDSYYGQ"
     gene            complement(3931..5628)
                     /locus_tag="VGQ51_19035"
     CDS             complement(3931..5628)
                     /locus_tag="VGQ51_19035"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEV9038743.1"
                     /translation="MRKNLVIGVDYGTDSVRSLLIDAADGSEIASSTFYYPRWKDGKF
                     CNPADNRFRQHPLDYIEGLESTIKDCLQQAGPAAARSVRAISVDTTGSTPVAVDASGT
                     PLGLLPGFGSNPNALFVLWKDHTSVKEAAEINAHATKTPVNYLQFVGGVYSSEWFWAK
                     LLHILRMDDDVRKACYSWVEHCDWIPFLLTGGKSVSEMKRGVCSAGHKALWAKEFGGL
                     PPDEFFSSLDPLLKGFTSRLFTRTYTSDQPAGHLCEEWGQRLGLSTDVVIGVGAFDAH
                     MGAVGGQIEPYHLSKVMGTSTCDMLVAPQEEVEGLLVKGICGQVPGSIIPGMMGMEAG
                     QSAFGDAYAWFRDLLSWPLQQLVPDAAKFIDKIIPELSRQASVLQPDPDAPLALDWFN
                     GRRTPDANQMLKGAVTGLNLGTDTPRLFHSIAESTCFGAKAIVDRFISEGIPVKGLIG
                     LGGVAKKSPFIMQMMADILNMPLRIHASEQTCALGAAMFAATAAGLYPKVEDAMAAIG
                     QGFDNTYTPDKDRSFIYASRYEQYSRLGAFIENTTQSHDSQRSAHSDAASIQIADHV"
     gene            6526..8286
                     /locus_tag="VGQ51_19040"
     CDS             6526..8286
                     /locus_tag="VGQ51_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038744.1"
                     /translation="MLHRYLPSCLLLFSIFLSLRLFSRDPGDPFTDSIKKKLTAAQTP
                     ADKMHFLMVLSFSSVDMAQSKQYADQGIAVAQMSRDRLLITQAYLLKGQLYMRNAGLA
                     DNLIQAEMNFHQSEQLARDNGLDKQLVFSLCGLSDVWEYRRDNMKALSFSNQALSVAM
                     NTENDSAKERAYGSMGEVYQSMNEMLLALRNYLSAMDVAEKSHDDFLLRDSYALMDNF
                     YEGIHEYDKAIDYRIKVYGLDRKVWDQRMAADEYRLGDLFLQNKQQELAMDMYERSIR
                     LADTLHFPLLKINSYFRIFSMYIHDNEYAKGLNYLRSQQGVVDVLTQLGFGFYIHQIY
                     GMAYGEQGKNDSALYYFHLAEPDVVNKTVPETQYDFYKAFGNFYVKRSDYSNAIVCYN
                     KAFAKAAGAAQLEWEAESTDTLEDLYQRTGDFKNALTCTIRSAAERDSLRTQGRTTDL
                     MKLEVENDNRRRERQAREEELRIEHRHNVQYMGLTVGLVTLFILLVMLGRLSVSLSVI
                     RALGFLSFIFLFEFIILLADRPIQVVTGDEPWKILLIKIALGAGLVPLHHWLEHKVIH
                     YLSSRRRVGVAVSKKPAHAE"
     gene            8276..9289
                     /locus_tag="VGQ51_19045"
     CDS             8276..9289
                     /locus_tag="VGQ51_19045"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV9038745.1"
                     /translation="MPNSTERPDQTEKGVIGGTERPAPAMKVVAAFATVYLVWGSTYF
                     FIRLSVEHIPPMMVGVLRYLTAGVLLLAWCLMTGKPVFSRQAIGPAFVSGFLLLCGGN
                     GVLIWSEQYIPSSLAAILLASGPIWFVVLDRRMWRVNFRSRSTIAGMVMGFVGVLLLF
                     GDGAGKGGGIKGMEMFALGILILGSISWAAGSLYSKYRSAPVSNSVNAGWQMLLGGLV
                     FVVASLISGEWKGFQVRQVPMSSWLAIVYLVTLGSLAGYSAFVWLLQVRPATQVSTHA
                     YVNPVVAVLLGVFFAGEEMTPLQLLGLAIILCSVLMINLSKYVGAGREGAGGKWVGLG
                     RNK"
     gene            9331..>10638
                     /locus_tag="VGQ51_19050"
     CDS             9331..>10638
                     /locus_tag="VGQ51_19050"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEV9038746.1"
                     /translation="MLALKRHFWYPLLLLLSMDAWCQSAHLSFEHLGTLQGLSHSNVT
                     CTLQDSRGFMWFGTREGLNRYDGYTFTVYKHRLENDRSLSNNKITGIVEDANGYLWIG
                     TWGGGLDRYDRKTDAFAHFQHDPKNPSSLSSNLVLCLLLDSRGYLWVGTEDGGLNRMD
                     TAGHFEHFVHREGDKNSLSSNYIKAICEGPSHYLWIGTYGGGLNRLDPKTWTVWRFLH
                     DPHDRGTLGDDNIASLRADGHNRVWVGTYTGLDQLDRITGRFTHYDKPGPNYVTNTTV
                     NSLGEDGEGNLWIGTENNGLVVMDSAGGMHHYLHDDVDPSSLGMNSLYTIYRDSKKNM
                     WIGSFSGGIDFVNWDTRKFPHFRHNSSPYSLSDNHVLCIYEDLENNLWVGTDGGGLNL
                     FDRKTERCKHFLHDQANSNSIGGNYVLRVIEDHNRNLWIGTWAD"
BASE COUNT         2300 a         2396 c         3400 g         2542 t
ORIGIN      
        1 agggctggag cttgaacata ctcttgtcga tctggaaagc cttcggattg atcttcggcc
       61 ctgcgaggct gaaggcgatc atgacgagcg cggtgaacag gaaggcaagc cccatgcaca
      121 cctggaaagg gatctgccag acggtgacga gggcaccgtt ctgttctatc tgggtggggt
      181 atgctgtgta cagccaggta ttatggccca gcacctctgg tgcgttcgtg ttgaagaaga
      241 cgcagaggac gaagcccacg atgaggccgg cgatggcggc tgtgccggtg gtccttttcc
      301 agaacatacc gaacaggaac atggcgaaaa cgcctgggct gatgaacccg gtgtattgtt
      361 gaataaaggt gaagccacct tttccgctga tgccgaggac gtcggtccag gtgaagatga
      421 tgccgacgat catggcgact acgacgacga tgcggccggt ccataccatt ttctgttcgt
      481 cggcggcttt gtcgaagtat tttttatgga tgtccagtgt atagatcgtg gagatgctgt
      541 tgaccttacc ggcgagcgag gagacgatgg ccgcggtgag ggcggcgatg gccaggcctt
      601 tcagaccggc gggcaggaag cccaggatgg cggaataggc gtagtccttg ctacctcctt
      661 ccagattgag atgaccatgt ttatagagga cgaaggcggc gatgccgggg agcatgacga
      721 tgacgggcat gaggagcttc atgaaccctg cgaacaggat gccggtgcgg gcggtctgga
      781 gattagcgcc gagtgcgcgt tgcgtgatgt attggttgca gccccagtag ttgaggttga
      841 cgatccactg accggcggcg tacatcgcga gaccgggcag gatgagatat ttattaatat
      901 cgagctggct cgaagtggcg gtcggtttgt ggaagatcat cctgaagtgt tcgggggcgt
      961 ccttcatcat ggccgtgaag ccggcccagg cgctgtcgcc gagaccgaag cttttcgcaa
     1021 ccaccgtgag ggcgagatag gacgtggcga ggccgcctat gatgagtacg ctgacctgga
     1081 tgacgtctgt gtagccgatg accttcatac cgccgagggt gatcaggagc gccatgatag
     1141 ccagtacgat catgaccgta tggaggtatt cgccgccgag gaggccgttg atggcgacgg
     1201 cgccgaggta gaggatggag gtaagattga cgaagatgta gagcagcagc cagaagatgg
     1261 ccatgatcag cgagacggtc tcgttgtagc gcatcttcag gaattggggc atcgtaaaga
     1321 tcttgttctt gagatagacg ggcatgaacc agaccgcgat gatgatgagc gccacggcgg
     1381 cgatccattc ataggaggcc acggctatgc cggcgaagaa gccgtcgcca ctcatcccca
     1441 caaattgttc cgccgagata ttggaagcga tgagcgaggc gccgatagcc caccaggtga
     1501 gggagccttc cgcaaggaag aagtctttgg tatcggtggt ggctttcctt ttacggcgat
     1561 agatccagat gccgtagaag gccacgatga ggaaataaat aagaaagacg agcttgtcgg
     1621 agaagaccag gttgctgttc atgggcaatc gtttttgtgc gaatatggtt gggggttagg
     1681 ggtcaggggt taatttggtt aatatcgttg cgagtcagac gttttattta aggtgataat
     1741 aagcctcgtt ccagcgaagc tcgttgcgta attggtgaag atcggttttt tttccaatca
     1801 ggacaaactc gatgccggcc atatctgcga aatcgcgcat atgttcggca gtaaggtttt
     1861 ggctatagca ggtgtggtgg gcgcctcctg cctgtatcca ggccgtgcag cccgtctgca
     1921 tgtccggata gggtttccag agtacgcggg cgacggggag tcgtggcagg ttgtgggtgg
     1981 gagcgacggc ctcgacctcg tttacgagga ggcggaagcg gttacccata tcgacgatag
     2041 aggcgttgat agccgggccg gcggcggaat taaagaccag tcttaccgga tcggccttgc
     2101 cgccgatgcc gagcggatgg atctcgcagg acacggtgcc gtctgcgatg gaggagcaga
     2161 tctcgagcat atgggcgccg agcacgaggt tattgccggg ctcgaaatgg taggtgtagt
     2221 cttccatgaa ggaatttccg cccgggagac ctgtgcccat cgtcttcatt gcgcggacga
     2281 gggcggccgt tttccaatcg ccttcgccac cgaaaccgta tcctgcggcc atgaggcgtt
     2341 gtgcggcgat gccgggcagc tggaccatac cgtgcaggtc ttcgaaggta tcggtgaagc
     2401 ctttgtagtg gccttccgcg aggaaggttt tcagacccag ctcgatgcgg gcggcctcgc
     2461 ggagtgaggt gtgttgtgtt ttgcctttgc ggagggaatc tgccagcgaa tatttttctg
     2521 tgtattcgtc gatcagcgta tcgatggcct tatcggagat ggaggcgatg gtcgcgacga
     2581 gatcgccgac accgtgggtg ttgacggagt atccgaattt cagttcggcc tcgaccttat
     2641 cgccttctgt gaccgccacc tgacgcatgt tgtcgccgaa ccggacgaag cgtgcgccct
     2701 gccagtcgtg ccagccggcc gccgcgcgtg tccacgcgtt gatgcggtcg agggtttcgg
     2761 gggactgcca gtggccggtg acgaccttgc gttcgagacg catgcgtgtc atgatgaatc
     2821 cgaattcgcg gtctccgtgg gcgctttgat tcaggttcat gaaatccatg tcgatcgcgt
     2881 cccaggggat atcgcgattg aactgggtat gcagatggag caggggtttt tgaaggatgc
     2941 gcagaccgcc gatccacatt tttgcggggg agaacgtgtg catccaggcg atgatgccga
     3001 tgcaggttgc cgtgttgttg gcctctacgc aagtgtggta gatctcttcg ggcgacttga
     3061 cggtcggctt gaagacgact tttaccggga tatcggggga gtcgttgagg gctttggcta
     3121 ttattttgga gtgttcggct acttgtttga gtgtttcttc tccgtagagg tgctggctgc
     3181 cagtgacgaa ccagacttcg agttttttta gatccatggt aggtggtaaa tatttattgt
     3241 ccgtaatagg agtcgggacc gtgtttgcgt tcgtaatgtt ttttgatcag cgagtctttg
     3301 aggcgtggcg catctgcgcg tatctgttcg gtcagatagg ccatgcgggc gacggtctcg
     3361 aggacggcgc tgttatagac ggctttttcg gccgtcctgc cccaggtgaa gggggcgtga
     3421 ttgcccacca ggaccatttc cacctcctga tagcggagcg ctttgtcgcg gaaggcgttc
     3481 attatctgga agccggtctg gtactcataa tgacccagga tcatttcgtc gtgcatcggc
     3541 ggggcgcagg ggatatccac cgtattatga tcggcgtggg tagtgccgaa gatggggata
     3601 tcgcgtccgg cctgggccca ggctgtagcg taggtcgagt gggtgtgcgc gatgccgccg
     3661 atggtttccc agtgtttata cagaacggcg tgggtgaggg tgtcggagga aggccggagt
     3721 tttcctttta cggtcttgcc ttcgaaatcg acgatcacca ttttttccgg cgtaagatcg
     3781 tcatatggaa cgccgctggg tttgatggcg aagacgccaa ggccgcggtc tgccgcgctg
     3841 acgttgccga aggtgaagag gacgaggccg agtttgggaa gctgcatatt ggcttcgtag
     3901 gcttcttcct ggatgtgaga atatttcgat ttagacatgg tcggcaattt ggatggaggc
     3961 tgcatcggaa tgagcagacc tttgtgaatc gtgggattga gtagtgtttt cgataaacgc
     4021 gcccaaacgg gaatattgtt cgtatctcga cgcgtagatg aaggaccggt ctttgtcggg
     4081 cgtgtaggta ttgtcgaagc cctggccgat ggcggccatt gcgtcttcta ctttcggata
     4141 gagaccggcg gcggtggcgg cgaacatggc ggctcccagg gcgcaggttt gttcggatgc
     4201 gtgaatacgg agcggcatgt tcaggatatc ggccatcatc tgcatgatga agggcgattt
     4261 tttggctact cctccaagtc cgattagacc ttttacgggg atgccttcgc tgatgaagcg
     4321 atcaacgatg gctttggcgc cgaagcaggt ggattcggcg atggagtgga atagccgggg
     4381 tgtgtcggta ccgaggttga gaccggtgac ggctcctttc agcatctggt tggcgtcggg
     4441 tgtgcggcgg ccattgaacc agtcgagggc gagcggcgca tccggatcgg gttggaggac
     4501 ggaagcctga cggctaagtt cggggatgat cttatcgatg aacttcgcgg catcgggaac
     4561 cagttgttgc aatggccatg acaggagatc gcggaaccag gcataggcgt cgccgaatgc
     4621 cgattgtccg gcctccattc ccatcatgcc ggggataatg gagccgggta cctggccgca
     4681 gatgcctttg acgaggaggc cctcaacttc ttcctgcgga gcgaccagca tatcgcaggt
     4741 ggaggttccc atgaccttgc tgaggtggta aggctcgatt tgacctccga ctgcgcccat
     4801 gtgcgcgtcg aaggctccta ctccgatgac tacgtccgtg ctcaggccga ggcgttgacc
     4861 ccattcttca cagagatgcc ctgcgggctg gtcggaggta tatgtccggg taaagagacg
     4921 ggatgtaaaa cctttgagta atgggtcgag cgatgagaag aattcatcgg gcgggaggcc
     4981 tccgaattct tttgcccaga gagctttatg tcctgcggag caaacgccgc gtttcatttc
     5041 ggagacgctt tttccacccg tgaggaggaa gggtatccag tcgcagtgtt cgacccagga
     5101 gtaacaggcc tttctaacgt catcgtccat gcgaagtata tgaaggagct tggcccagaa
     5161 ccattcggag gagtacacac cgccgacgaa ctgaaggtaa ttaacgggtg tcttcgtggc
     5221 gtgagcattg atctcagcgg cctctttcac ggatgtatga tccttccaca gcacgaagag
     5281 tgcgttgggg ttggacccga agccagggag aaggcccagc ggggtacccg aagcgtctac
     5341 ggctaccgga gtggagccgg tggtatccac ggagatggct ctgacggacc gtgctgctgc
     5401 gggtccggcc tgttgcagac agtctttgat cgtcgattcg aggccttcga tatagtcgag
     5461 ggggtgttgt ctgaaacggt tgtcggcggg attgcagaac ttcccgtctt tccagcgggg
     5521 ataataaaag gtggaggagg caatttcgga accgtcggct gcgtcgatca ggagggatcg
     5581 gacggaatcc gtgccataat ctactcctat cacaaggttt tttctcatat ggtgagttgc
     5641 gaattcctgt caaaataaca tttatttttt cgtaccgaaa aaatttattt gcgggggcgg
     5701 aggggggcat tcgagggagg tagcgggatc attcgaggga ggtaggggga tcattcgaag
     5761 gagatggagg aatcattcga gagaggtagc gggatcattt gaggtcgggg aaggggtggg
     5821 tcggttggat gacagttcat cggggagata ggaaggagat gacggccggg ggaggtgagg
     5881 tggagggcga ttgtggcgta aacgtagggg atcggagggt gggtggaaaa aatcactgca
     5941 ggatagatgc agggaataac gaaaaaggtt gtaatttctg accctgtggt ggtggggcgg
     6001 gccgattttt tgcagggagc aattgggaaa aggcaaaaaa atcctggtaa tttttaagat
     6061 acgtaacaat tattttatga tacttcatac agatcgaaaa atatcaatta cgtatctggt
     6121 aaaatcgaaa acattgattc ctagaagttt atctaaggtt aagtctattg gtgaatacca
     6181 gaaaagccct aaattgtctt gcccctcaaa ccgccccgga aatggaaaaa aagcttaaaa
     6241 tcaacaggat caaagcagtc ttagctgaga gagatatctc ccataaagat ttggcgatca
     6301 gggtcggcgt agctcccaac agtgttacaa ggatctgtaa caatgaattt cagcctactt
     6361 taaggctact tcgggagatc gcgctggccc ttgacgtgga tatccgtgaa ttattgatac
     6421 caacgcgtca ggtcacctaa actattagtt ttttataata tgttgaagga tcatccggac
     6481 tggcgtccgg gatggtatat ttgggcagtc tattaaacca atgctatgct gcaccgatat
     6541 ttaccgtcct gtcttctcct attttctatc ttcctctctt tgaggctatt ctcccgggat
     6601 ccgggagatc cttttaccga cagtatcaag aaaaagctga cagcggcgca gacacccgcc
     6661 gacaagatgc atttcctgat ggtcctgagc ttctcgagtg tagacatggc ccaatcgaag
     6721 caatatgccg atcaggggat cgcggtagca caaatgagca gggaccgtct gctgatcacc
     6781 caggcgtatc ttctgaaggg acaattgtac atgcgtaatg ccgggcttgc ggacaacctc
     6841 attcaggcgg agatgaattt ccaccagagc gaacagctgg ccagggacaa cgggctggac
     6901 aagcagctgg tatttagtct ttgcggtttg tcggatgtct gggaatacag gagggacaat
     6961 atgaaggcct tgtctttcag caatcaggct ttgtcggtgg ctatgaatac ggagaacgac
     7021 tccgcgaagg aacgggcgta cggctcgatg ggcgaggtat accagagcat gaacgaaatg
     7081 ttgctcgcct tgcggaatta cctgagtgcg atggatgtcg ccgaaaaaag ccatgacgat
     7141 tttcttcttc gggacagcta cgcgcttatg gacaactttt acgaaggtat ccatgaatac
     7201 gacaaggcta tcgattaccg tataaaggtc tatggactcg acagaaaagt gtgggatcaa
     7261 cggatggccg cggatgaata tcgattgggg gatctgtttt tacaaaataa gcagcaggaa
     7321 ctggcgatgg acatgtacga acggtccatc cgtcttgcgg acacgctgca tttccctttg
     7381 ctcaagatca acagctattt ccgcatcttc agtatgtata ttcacgacaa tgaatatgcg
     7441 aagggcttga actatctccg gtcgcaacag ggggttgtgg acgtccttac ccagctcggt
     7501 ttcgggtttt atatccacca gatatacggt atggcttatg gcgagcaggg gaagaacgac
     7561 agcgccttgt attatttcca tcttgcggag cccgatgtgg tgaataagac cgtcccggag
     7621 acccaatacg atttttataa ggccttcggt aatttttatg tgaagcgaag cgattactcc
     7681 aatgcgatcg tttgttacaa caaggcattc gccaaggccg ccggagcagc gcagctggaa
     7741 tgggaagcgg agagcaccga tacgctggag gatctatacc aaaggacggg ggattttaag
     7801 aatgcgctga cctgtacgat ccgttcggcc gccgagcggg acagtctccg gacacagggc
     7861 cggaccaccg atctgatgaa gctggaggtg gagaatgaca atcgccgcag ggagcggcag
     7921 gcgcgggagg aggaattacg gatagagcac cggcacaatg tacaatacat gggtttgacc
     7981 gtggggcttg tcaccctgtt cattttactg gtgatgctcg gacgtctgtc ggtatcttta
     8041 tccgtgatcc gggcgttggg ctttttatcc tttatctttc ttttcgagtt cattatcctg
     8101 cttgcggacc ggcctatcca ggtggtgacg ggagatgagc cgtggaagat cctgctgatc
     8161 aagattgcgc ttggtgcggg gttggtgcca ttgcatcact ggttggagca taaagtcatt
     8221 cattacctga gcagccgcag aagggtgggt gttgctgtct cgaaaaaacc tgcgcatgcc
     8281 gaatagtacc gaacggccgg atcaaacgga aaagggggtg atcgggggta ccgaacggcc
     8341 ggcccctgcg atgaaggtgg ttgccgcatt cgccacagtg tacctggttt ggggctcgac
     8401 ctattttttt atccggctgt cggttgagca cattcctccc atgatggtgg gggtgcttcg
     8461 gtatttgact gccggggtgt tgttattggc ctggtgtctg atgacgggta aaccagtgtt
     8521 ttcgcgacag gcgatcgggc ccgcctttgt aagcggattc ctgttattgt gcggagggaa
     8581 tggggtcctg atatggagtg agcaatatat tccgagcagt ctcgcggcca tccttttggc
     8641 gtcgggtccg atatggttcg tcgtgctgga caggcgtatg tggcgggtga attttcgcag
     8701 ccggagtact atcgccggga tggtcatggg ttttgtcggg gttttgctgt tgttcggcga
     8761 cggggctgga aaggggggtg ggataaaggg tatggaaatg tttgcgcttg gaatattgat
     8821 ccttgggtcg atctcctggg cggcgggttc gctttattcg aaataccgga gtgctccggt
     8881 ctcgaattcc gtgaatgccg gttggcagat gttactaggc gggctggtgt tcgtggtggc
     8941 gagcctgatc agcggcgagt ggaagggttt ccaggtgcga caggtaccaa tgtcttcatg
     9001 gcttgcgatc gtatacctgg tgacgttggg ttcgctggcg gggtacagtg cgttcgtgtg
     9061 gttattgcag gtgagaccgg cgacgcaggt gagcacacat gcgtatgtca acccggtggt
     9121 ggcagtgttg ctgggggttt ttttcgcggg ggaggagatg acgccgctgc agctgctggg
     9181 gctggcgatc atattgtgta gtgtgttgat gatcaacctt tctaagtatg tgggagcagg
     9241 cagggagggt gctgggggta aatgggtagg cttaggccgc aataaataac cctggcgggc
     9301 gttttcaacc taaccccgtc tgcctattgt atgcttgctc tgaaacgcca tttttggtac
     9361 cctttactgt tgctcctgtc aatggacgca tggtgccagt ctgcgcatct ttcttttgaa
     9421 catctgggaa cgctgcaggg tctttcgcac agcaatgtca cctgtacttt acaggacagc
     9481 cggggattca tgtggtttgg gaccagggaa ggattgaacc ggtatgacgg gtatacgttt
     9541 accgtatata agcatcgttt ggaaaatgac agaagtctca gcaataacaa gataacggga
     9601 attgtcgagg atgcgaacgg ctatctctgg atcggcacct ggggaggagg acttgacagg
     9661 tatgatcgaa agaccgatgc ctttgcgcat ttccagcatg acccgaagaa tccttcgagt
     9721 ctttccagca acctggtact ttgtctgttg ctggacagca gaggttatct atgggtgggt
     9781 acggaggatg gtggtctgaa ccggatggat acagccggtc atttcgagca tttcgtacat
     9841 cgggaagggg acaagaatag tctgtccagc aattacatca aagcgatctg cgaggggccc
     9901 tcacattatt tatggatcgg aacctatgga ggcgggctga accggctgga cccgaagacg
     9961 tggaccgttt ggcgattcct gcatgatccc catgaccggg gtacactggg tgacgacaat
    10021 attgcttccc tccgggcgga cggtcataac cgggtgtggg tcggcaccta tacggggctg
    10081 gatcaactgg atcggatcac cggaagattc acgcactacg ataagcccgg acctaactat
    10141 gtcaccaaca cgacggtcaa ctcgctgggg gaggatgggg aaggcaatct ttggatcggc
    10201 acggaaaata atgggttggt cgtgatggat agcgcggggg gcatgcatca ttacctgcat
    10261 gacgacgtgg acccatcgag cctggggatg aactcgctct atacgatcta ccgggattca
    10321 aaaaagaata tgtggatcgg ttccttctca ggtgggatcg acttcgtaaa ctgggatacg
    10381 cggaagttcc cgcatttccg gcataacagc agcccctata gtctcagcga caaccatgtg
    10441 ctgtgcatct atgaagatct cgaaaacaac ttgtgggtcg gcacggatgg aggagggctc
    10501 aatcttttcg accggaagac cgagcggtgc aagcatttcc tgcatgatca ggccaattcg
    10561 aacagcatcg gcggcaacta tgtgttgcgg gtgatcgagg atcacaatcg caacttgtgg
    10621 atcggtacgt gggccgac
//
LOCUS       DASYSV010000610         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1531479, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000610 DASYSV010000000
VERSION     DASYSV010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531479"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VGQ51_19055"
     CDS             complement(<1..543)
                     /locus_tag="VGQ51_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV9038747.1"
                     /translation="MTKVIFTPEQARKIIIHAAGLARRAQFGKGREAVYKLIEHLGFI
                     QLDTNYTVERAHHHVIAARVPDYRLEWLDELQEDGRVFEFFTSDAGYIPMKDFRFSLP
                     VKQAFASNRKPMTPAEISLMNKVLDRVAREGPQMVKDFDNDRQEASAGWWDWRPSKIA
                     LERLYLDGRLMTTRTKGFQKI"
     gene            1163..1384
                     /locus_tag="VGQ51_19060"
     CDS             1163..1384
                     /locus_tag="VGQ51_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038748.1"
                     /translation="MRALVYVNDFLYLYEIEPEDKKLKSLYGVKFITQQRVDFLDIRC
                     HHPEKGHLDYECAIMAGLQQHLTSNRVIG"
     gene            complement(1718..2521)
                     /locus_tag="VGQ51_19065"
     CDS             complement(1718..2521)
                     /locus_tag="VGQ51_19065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV9038749.1"
                     /translation="MHDLILYTGIFLTVGSFYYFILSKKFDGWAFRHSLKLPIAKKHP
                     AGHVFALANIAVAGFFQSLVFAGLCCYFATPWKSILVDNFRPIQLAYGIFLGLADIVA
                     CNMGGLLFMRAWQSIRSRGAKNALAGSQKQVMSGWVNSFKLSFENFPLAVAGPIAFFY
                     ILFEELIFRGVVLFAYAGISGIPVFLAILLSTLLFTLAQRPGIPRARDANYPMRSAFI
                     MGLIHNWLMLKVFSIVPLIIAHFVFLLVATLPVKKTGNKLTPNADLLGI"
     gene            complement(2524..>3171)
                     /locus_tag="VGQ51_19070"
     CDS             complement(2524..>3171)
                     /locus_tag="VGQ51_19070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV9038750.1"
                     /translation="ILSTYAAGRLEISRPTVYWISYLSILLFIAGTGSVFLLNGSLFG
                     CSVQGILLSVSVVALAVLFESTLRTKGAAAGRRKPDNRACQDIPTSSYLLTDQLKKHA
                     WIIKGKQTVIRWIIIFALIGCLEEICYRGIILQTILYKAVGAPRALLICLQVILFGLN
                     HVFFGARQVLMKTLLGLLLTVITLVTGTLVPAMLAHSLFNAWAIYDSNKQLLKAA"
BASE COUNT          805 a          775 c          784 g          807 t
ORIGIN      
        1 tattttctgg aagcctttcg tcctcgtggt catcagtctt ccatcgagat agagcctttc
       61 aagcgcgatc ttggacggtc gccagtccca ccaacccgcg cttgcctcct ggcgatcgtt
      121 gtcaaaatcc tttaccatct gcgggccttc acgcgccacc ctgtccagca ccttgttcat
      181 gaggctgatc tccgcgggag tcatcggttt ccgattggac gcgaaggctt gttttactgg
      241 caatgaaaaa cggaagtctt tcatgggaat atatccggca tcggaggtga agaattcaaa
      301 gaccctgccg tcctcctgta actcgtccag ccattccagc ctgtagtcgg gtacacgcgc
      361 ggcgataaca tggtgatgcg cccgctcgac tgtataattg gtatccagct ggataaaacc
      421 gaggtgctcg atgagtttgt acaccgcctc tcttcctttg ccgaactggg cgcgccttgc
      481 gagccctgcc gcgtggataa tgatcttcct ggcttgctcc ggtgtaaata ttaccttcgt
      541 cataaccatt aaaattagct aaattcgcag aagttaccgg ggaaaggcac aattcttgat
      601 gtgatattga acggtttata tcaataaatc acaatttgtt cgagttgtcc cgcttccgga
      661 aactctcgct aacgacctaa gaaataatgt ccgcgatccg aaggaaatat gtaaagcgcg
      721 cccgcaacgg cgtgctcatt atagcccatc tggctgtatt gggtttacaa ttctattacg
      781 ggtactacca attcttcggc cattccactt atcggtcgct ttttttatca cattcgacaa
      841 cggcaacaca ctcaatccgg cattcgcgca ttggctggga taagaattcc cggagggccc
      901 tgctgctgga taagcgcttt gcgcatgagc atgtatggtg ttgtcctacc ccgttttttt
      961 tcctgccctt tcaagccgat gaccagcaac ggtctttctg ctccagccgg atatatgcca
     1021 ggatgggctg gttgcgtctt ttcctccgtg gtccacctga tgatcgaaca atagtttgac
     1081 atcatcttca ctgaccaaac cagcaattta ctggatgtat tatttttatt tttcctgcac
     1141 cttcgaagaa attcaaaggc gaatgcgggc gcttgtatac gtcaatgatt tcctctatct
     1201 ctacgaaata gagccggagg acaaaaaatt gaaatcactt tatggggtga aattcattac
     1261 tcagcaaaga gtggatttcc tcgacatccg atgtcatcac ccggagaagg gacatctgga
     1321 ctatgaatgc gccataatgg cgggtttaca acaacactta acgtccaata gggtaatagg
     1381 ataagcagag gctggtcggc ccttttaaac aatctttcat tgacacctcc tgttagaatt
     1441 gtgttgatga agatgaaatc gaccagcctc tttgctgtga ttgggtgtga tttacaggta
     1501 ccatatttga agctttttga tcacgagggc cttgcggctc ttttagtcca atgctgcatg
     1561 ccgggtggta agcccggctg ttttcgttcg cttcacaggt ttgaaaggac ctattccctt
     1621 tagttaaaaa ggacaacgcc gcgacggcgg tttctaacca ttattgcgat tgttttcgca
     1681 atcgtcttga ttatcaggca ttggcagatc ggctttgtca tattccaaga agatcggcat
     1741 ttggtgtcaa tttgttgcct gtctttttga cgggaagggt cgccaccagc agaaatacga
     1801 aatgggcgat gatcagcggg acgatcgaga agacctttag catgagccaa ttgtggatga
     1861 gccccattat aaaggctgaa cgcatcggat agttggcgtc gcgggccctg ggaatgccgg
     1921 gtctttgcgc caaggtaaag agtagggtag acagaaggat cgccaggaag acggggatcc
     1981 cgctgatccc ggcatacgcg aataaaacga ctccgcgaaa gatcagctct tcgaaaagga
     2041 tataaaaaaa ggctatcgga cccgccaccg caagagggaa attttcgaat gacaacttga
     2101 aagaattgac ccagccgctc atgacctgtt tttgtgatcc ggctaaggcg ttttttgccc
     2161 ctcggctgcg gatggattgc caggctctca taaatagtaa gcctcccata ttgcaagcca
     2221 cgatatccgc caaccccagg aaaataccgt atgccaattg gatcggtctg aaattatcga
     2281 caaggatcga cttccagggg gtcgcaaaat agcagcacag gcctgcaaat acgaggcttt
     2341 gaaaaaagcc ggcaacggcg atgtttgcca gcgcgaagac atgaccggcc ggatgttttt
     2401 tggcgatcgg aagtttgagc gaatgtctga atgcccagcc atcgaatttt tttgacagga
     2461 tgaaataata gaaggaaccc actgtcagaa aaatgccggt atataggatg aggtcatgca
     2521 tgactatgcg gcttttaata gttgcttatt ggagtcatag attgcccatg cattgaacaa
     2581 cgagtgcgcc agcatggcgg gaacaagggt tccggtcacc agggtgataa cggtaagcaa
     2641 gagtcccaga agcgttttca tgagtacttg cctggcgcca aaaaaaacat ggttcaaccc
     2701 aaacaatata acctgtaaac agatcagcaa tgccctcggg gcgccgaccg ccttgtagag
     2761 gatggtctgc aaaataatcc ccctgtaaca tatctcctcc agacagccga tgagagcgaa
     2821 aatgatgatc catcggatga ccgtttgttt gccttttatg atccatgcat gttttttcaa
     2881 ttggtcggtc aaaagatagg aactggtcgg gatgtcctga caggcccggt tatccggctt
     2941 tcttctcccg gcggctgccc ctttcgtccg gagcgtcgat tcgaacagca ctgcgagcgc
     3001 gacaaccgaa acagacaaca gtatcccctg cacggagcag ccgaacaacg atccgttcag
     3061 caaaaagacg gaccccgtgc ctgcaatgaa gaggagtatg ctcagatagg atatccaata
     3121 gacggtcggc ctggatattt caagtctccc tgcagcgtat gtggaaagaa t
//
LOCUS       DASYSV010000611         9806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2556223, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000611 DASYSV010000000
VERSION     DASYSV010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9806
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556223"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VGQ51_19075"
     CDS             complement(<1..1159)
                     /locus_tag="VGQ51_19075"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9038751.1"
                     /translation="MNKYKINYLGVLMLGAGLAFGSCSKSFVDKSSSTNLSTSEALST
                     PELLKTDLNGLYAQLRNVDLFGRDFPVLGDLEADNTFLEDRNTGRYNYQFAYSVPVTD
                     NVTQAMWQQSYSGILYANQIIDAPVTGSDAAVTKAQAYALRALLYFKLVNIFAQPYTF
                     DSTGMGVPLVLHYDVTATPGRATVAQVYHQIISDLRTSMVSAPEYTSSIFISKYAVEG
                     LLARAYMYMGDYGDAAVAAVDVINNSPFTLVSAGAFRSFWADAGVQTSATEVMFEVDA
                     DAINNNGFDDLGGIYFNGYQDIYCSLDLAELYSATDVRTQILYSGFTKGGQPAILVGK
                     FPNAQSTDRDNLKVIRLAEVYLIAAESEARLGDNADAQDYVNALAETRDPVF"
     gene            complement(1178..4219)
                     /locus_tag="VGQ51_19080"
     CDS             complement(1178..4219)
                     /locus_tag="VGQ51_19080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEV9038752.1"
                     /translation="MRRFLLFLCCFAFLIGQVTAQNHTVSGTITDGNGVPVAGASVTL
                     KGSSRGTSTATDGTFTLAVPQSARTLVLSAVNLSTVEIDISDGKTSIGTIALQAAGKN
                     LSEVVVVAYGTQNKVNVTGSVATVSGNVVADKPFTSVDKALQGAVPGMLVSSTSGAPG
                     SATDIIIRGVGSINASAQPLWVIDGAIATSGDLTINTTTANILSTLNPDDVESISVLK
                     DAVSTAPYGSRGANGVILVTTRKGRAGKTHFSVVGEFGQNNRAYSPSNIPENSTGLQT
                     TLRQALINAGYVGSNSDADQLITQAFGYPANYTSINTNWFDVVSQQGAQSQVSMSLSG
                     GNDKTTVYASGGFFDQKGISLASDFRRFAGALAVTHHASDKFTLTANLNASNTAQHTP
                     SNGGTFANPVLASFFLLPWFTPYHPNGQFRYGANDTLNEFPQTGAIFNPVVQAAWNTN
                     LAQQTAIRGSAMGEYKFLSNLKLTSRFSSEYLTVQEDQYRNPFYGDGFAAGGDAASNY
                     YRVFDYTWSNFLDWKAKINPDGDEYFDLKGGIEAYDNKSYTLNAGGHSFPQTLAVKYL
                     SNTAVPTVALASPAEQTVFSEFAIGDFNYKDRYILSGSIRRDESSVFGADNRWGTFYS
                     VGGSWNLNEEQFMKNQDLFDLLKLRVSYGQTGNTNGFGLYTSMPQYASFNYTTQAGLI
                     PSNVGDSALTWEKNVALNIGLDFAILKDRIDGTIEYYHRTTNDLLSPVPFSFTSGFSQ
                     QNENIGSVVNKGIEIAINGKPVWTKNFTWEINFNISHNTNRVISLYQGKPIPNGSFEY
                     TVGHDLQEYYLQQWAGVNTQTGQAQWYTDGTHQKLTNNYDSAGLALNHSAAPSVFGGL
                     TNTFTYKGFTLDFQFDYSFGNYIFDNWYNYLNSDGGYAGSFNQMSTQLNAWQKAGDKT
                     DVPQIVYGDPSFSSAPSTRWLYKGNYIRLRNLQFGYDIPQAVLKKAHMDRVFIYIRGT
                     NLATFGTDKNLPYDPEAGYNSQANLEVLIPKTIAAGVKLGF"
     gene            4358..5260
                     /locus_tag="VGQ51_19085"
     CDS             4358..5260
                     /locus_tag="VGQ51_19085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV9038753.1"
                     /translation="MKKTLSSCFAHLIAAGLIAAGLLTGCGNPGNTSDKDTTATAAAA
                     DTIDVHTDWKIGVQLWTFHYVPFVQAIEKADSAHVKYLEAIPGQPLGGGMKDSFGIDM
                     SDASKAKIKKLLGSKGMQIVAVGVIGPSSVAEWKRWFDLAKEFGLSYITCEPLREHWN
                     AIDSLAGIYGINIAIHDHPKPDPYWHPDSVLAAIAGHKHIGACADVGHWTRNGLDPVD
                     CLKKLAGHIYGVHLKDVDSAGNVNANDKVVGTGVISFPPIFQELKRQRFKGMFSIERE
                     GNWYNNVPDVTATIRFYNEQLSKL"
     gene            complement(5353..6756)
                     /locus_tag="VGQ51_19090"
     CDS             complement(5353..6756)
                     /locus_tag="VGQ51_19090"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9038754.1"
                     /translation="MNNRKRYILFAGIGLFFLSSCSKNFVNKSPYSSTTLSEALGSVS
                     QLQTALNGAYAQMRTVALYGRDMPVIGDLQADNTFVEVDNSGRYLNQFKYTFTNADGV
                     VEGDWNAAYQGIQEANQVIDAEVTGAEAIKAQAYALRGLLYFKLVNMYARPYTDNPNA
                     LGVPLILHYQPSLLPKRDSVGKVYAQIVSDLKAAFQNAPAYSNSGLISKYAAEALLAR
                     TYLYMGDNTNAKAAAVDVINNGGFTLAQTAGAYAAFWSNPARKSDKVEVLYEVDADAV
                     NNNGTDGIDAMYMGGYTDIYASQDLVNLYSATDVRNQVLVPGTTKSGATATLVGKYPN
                     YTSTDKDNPKVIRLAEVYLIAAEASLPGNEADARMYLNDLMAVRDPAFAGYSSTGAQL
                     RSDILTERRKELAFEGDRYYDLNRLKMDIVRPAINSGSINGPLTMPYSDYRRVYPIPL
                     NEIQANANIASQQNPGY"
     gene            complement(6782..>9806)
                     /locus_tag="VGQ51_19095"
     CDS             complement(6782..>9806)
                     /locus_tag="VGQ51_19095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9038755.1"
                     /translation="ALLFGQLAAQNQHTISGTVTDAMGSPVAGATIGIKGTNRATATG
                     NDGTFLISVSGKDKTLVISAVNFQTQEIDIAGKTSIGTVNLARGTKNLDEVVVVAYGT
                     LKKTNITGAVGQINGSQIADKPLASVDQALQGAAPGLMISAPNGIPGAAVDVRIRGIG
                     SISASASPLWVIDGAVATSGDLATTSTSTNALSGLNPDDIESISVLKDAAATALYGSR
                     AANGVIIVTTKKGKAGKTRINFTTEVGKNSVAFANKNNRPLTTPQYHQLLDLATINGG
                     YANTNAEADAFIIDPNNGLGLPADWTKTNTDWLHQVTRTGNQQQYNLSLSGGNEKTQY
                     FASAGYFDQDGTTIATNFKRYNGNVSVTNKVNDKILFNVGINGSYSYQIAPPGSAAYA
                     SPVSGAFFLPPYYSPYNADGSLRFNDPEGEFPEGAQFNPLAIAAFNRYTDGQTELRGY
                     VSGEYQILDNLKFTSKYSAEFLDANEYLYWNPIYGDGYPLGYGIATDRKIFDWTWTNQ
                     VNYRANLNQDKDFYLDLLAGTEAYNLNDYSIQAQGQNFPSTTLLTYLASAAAPVSAYN
                     LPVSNSLNSYFSQAVFNYQDRYILSGSYRLDGSSKFAQGHKWSSFWSVGGSWNINEEQ
                     FMKGMSGFNLLKLRASYGTAGNTNGFDDYAALAQYGYGNPYEGNAGSTLNNVGNPNLT
                     WELNKSFNVGLDWGIWKDRLGGSVDYYKRTTSQLLALVPLSATAGVPGSTQLQNVGSM
                     YNQGIEITLTGKPILTKDFTWTSTINFSHNKNRVTELYNHSPISQNTRYNITEGHDMH
                     EFYTRLWDGANPANGDPQWFTDATRKTVTSTSNQVKLSLTGKLGTPQYFGAWTNTFQY
                     KGLSLTAQLYYNFGNYVYSTWENYLQSDGLYLPYFGQLSNELNGWTHAGQITNVPRQV
                     LGGNKNSNVPSTRYLYKGNYIRLRNIELAYSLPKSTMSRAHINNIMIYVRGTNLLTFA
                     TDKNLAIDPELGVNSLADFQVFTPKAITAGIKLGF"
BASE COUNT         2054 a         2666 c         2768 g         2318 t
ORIGIN      
        1 taaagaccgg gtcgcgggtc tcggccagcg cattcacata atcctgcgcg tcggcattgt
       61 cccccagccg cgcttctgac tctgcagcga tcaaatagac ttccgccagc cggatcactt
      121 tcaggttgtc cctgtcagta ctctgcgcat tggggaattt gcctaccagg atcgccggct
      181 gcccgccctt cgtaaatccg gaatacaata tctgcgtgcg tacgtccgtc gcactgtaca
      241 gctccgcgag gtccaaagaa caatagatat cctggtatcc attgaaatag atgccgccca
      301 ggtcgtcgaa accgttgttg ttgatggcat cggcgtctac ttcgaacatc acttcggtgg
      361 cgctggtctg tacgccggca tcggcccaga agctcctgaa ggcgccggca ctcaccagcg
      421 tgaacgggct gttattgatg acatcgacgg ctgcgacggc ggcatcgcca taatcaccca
      481 tatacatata cgcccttgca agcagaccct cgacggcata tttgctgata aagatcgagc
      541 tggtatattc cggcgccgat accatcgacg tcctcagatc actgatgatc tggtggtaca
      601 cctgcgcgac ggtcgcacgt ccgggcgtgg cggtgacgtc gtagtgcagc accagcggta
      661 cgcccattcc cgtactgtca aacgtatagg gttgcgcgaa gatattcacc agtttgaaat
      721 agagcagcgc gcggagggca taggcctgcg ccttcgtgac ggccgcgtcc gaccctgtta
      781 cgggtgcgtc gatgatctgg ttggcgtaca gtatgcccga ataggattgc tgccacatgg
      841 cctgggttac gttgtccgtg acgggtaccg agtaggcgaa ctgatagtta taacgaccgg
      901 tattgcggtc ttccaaaaag gtattgtccg cctccaggtc gcccaaaacc gggaagtcac
      961 ggccgaacag atcgacattg cgaagctggg catagagccc gttcagatcg gtcttcaaca
     1021 gctcgggcgt gctcagggcc tcgctcgtcg agaggttggt ggacgaggat ttgtcgacaa
     1081 aggatttcga acaggacccg aatgccagcc cggcccccag catcaataca ccgagataat
     1141 ttattttata cttgttcatg ctaattcagt tttgaggtta aaaaccgagt ttgacacctg
     1201 ctgcaatcgt ttttgggatc agcacttcca ggttggcctg gctgttatag cctgcctcgg
     1261 gatcataggg caggttctta tccgtcccga aggtcgccag gttggtgccg cggatataga
     1321 tgaagacgcg gtccatatgc gcctttttca gcacggcctg cggtatatca tacccgaact
     1381 ggagattccg cagacggata tagttgccct tgtatagcca acgcgtggaa ggagcactcg
     1441 aaaagctcgg gtcgccatat actatctgcg gcacgtcggt cttgtccccc gccttttgcc
     1501 aggcattcag ctgggtgctc atctggttga agctgcccgc atacccaccg tcgctgttca
     1561 gatagttgta ccagttgtcg aagatatagt tcccaaaact atagtcgaac tgaaaatcga
     1621 gcgtgaaccc tttatacgta aacgtattgg taagacctcc gaatacggaa ggcgccgccg
     1681 aatggttcaa ggcgagcccg gcactgtcat aattgttggt gagcttctga tgcgtcccgt
     1741 cggtgtacca ttgcgcctgc ccggtctgcg tattcacacc ggcccattgc tgcagatagt
     1801 attcctgcag atcatgaccc acggtgtatt cgaaacttcc attcgggata ggcttaccct
     1861 ggtacagcga gatgacccgg ttggtattgt gcgatatatt gaagttgatc tcccaggtga
     1921 aattcttggt ccataccggc ttgccgttga tcgcgatctc gatcccttta ttgacgacgc
     1981 tgccgatgtt ctcgttctgc tgcgaaaacc cggatgtgaa ggaaaagggc acgggcgaca
     2041 aaagatcgtt cgttgtccga tgatagtatt cgatcgtacc gtcgatgcga tccttcagta
     2101 tggcgaaatc gagtcctata ttgagggcta cgttcttttc ccaggtgagc gcgctgtcac
     2161 ccacattcga gggtatcagg cccgcctgcg tggtatagtt gaaggaagca tattgcggca
     2221 tcgaggtata caggccgaac ccgttggtgt tgccggtctg tccgtaagag acccggagtt
     2281 tcagcagatc gaacaggtcc tggttcttca tgaactgctc ctcattgagg ttccagctgc
     2341 ccccgacgga ataaaacgta ccccaacggt tatccgcacc gaaaacggaa gattcgtcac
     2401 ggcggatgct gccactcagg atgtagcggt ccttataatt gaaatccccg atcgcaaact
     2461 ccgaaaagac cgtctgttcc gccggcgaag ccagcgcgac cgtgggcacg gccgtattgg
     2521 aaaggtattt taccgccagc gtctgcggaa aggagtggcc ccccgcattg agcgtataac
     2581 tcttgttgtc atacgcctct atccctccct tcagatcgaa atattcgtct ccgtcggggt
     2641 tgatctttgc tttccagtcc aggaagttgg accaggtgta atcgaagacc cggtaatagt
     2701 tggaggcggc atccccgccg gccgcaaacc cgtcgccata gaaagggttg cgatactggt
     2761 cttcctggac ggtcaggtac tctgacgaga accgcgaggt cagcttgaga ttggaaagga
     2821 atttgtattc gcccatcgcg ctgccgcgga tggcggtctg ctgtgcaaga ttggtattcc
     2881 aggccgcctg gacgacggga ttgaagatgg cgccggtttg cgggaactcg ttcagcgtat
     2941 cattggcgcc ataccggaac tgcccgttcg gatgataagg ggtgaaccag ggcagcagga
     3001 aaaaggaggc cagcacgggg ttggcaaagg tgccgccatt gcttggtgtg tgctgcgcgg
     3061 tgttactggc attcaggttc gccgtcaggg tgaatttgtc gctggcatga tgtgttaccg
     3121 ccagcgcacc ggcaaatcgt ctgaaatcgg aggcgaggct gatgcctttc tgatcgaaaa
     3181 agccacccga cgcatagacg gtggtcttat cgttgccgcc gctgagactc atcgacacct
     3241 gcgactgcgc tccctgttgc gagacgacgt cgaaccagtt ggtattgatc gaggtataat
     3301 tggcgggata accgaaagcc tgcgtgatga gctggtccgc atcgctgttg gagccgacat
     3361 acccggcatt gatcaacgcc tgccgcaagg tcgtctggag acccgtggaa ttctccggga
     3421 tattggaagg cgagtaggcc cggttgttct gcccgaattc gcccacaacc gaaaaatggg
     3481 tctttccggc ccggcccttt cgcgtggtga cgaggatgac cccattggcg ccccgggatc
     3541 cataaggcgc ggtagagacg gcatccttca ggaccgatat gctttcgaca tcgtcggggt
     3601 tcagcgtgct gaggatattg gcggtcgtgg tattgatggt cagatcaccg ctggtcgcga
     3661 tcgcgccgtc gatcacccat aagggttgag cgctcgcatt gatcgaccct acaccccgga
     3721 tgatgatgtc cgtggccgac ccgggcgccc cggatgtcga agataccagc atgccgggta
     3781 ctgccccctg taatgccttg tccacggagg tgaagggctt gtcggcaacc acgttcccgc
     3841 ttaccgtcgc caccgatccc gtgacgttga ccttattctg cgtcccatag gctacgacca
     3901 ccacctcgct gagattcttg ccggcagcct gcaaggcgat cgttccgatg gaggtcttgc
     3961 cgtccgagat atctatttct accgtagaca gattgacggc agagaggacc agcgtccggg
     4021 cggactgcgg aacagccagc gtaaaggtcc cgtccgtggc ggtgctggtc ccccggctgg
     4081 agcctttgag ggtaacgctc gccccggcta ccggtacgcc gttcccatcc gtaatagtac
     4141 ctgaaactgt gtgattttgc gctgtgacct gtccgatcag aaacgcaaaa cagcatagga
     4201 agaggagaaa tcttctcata catattagtt ttgtgtgaag taatgtcgac caattcggta
     4261 tactaattta acgatataca tactctccgg gttgtctatc aagaccgctt ttatccagaa
     4321 ttcgctgttt gcgatataaa aaggctaatt tcagcccatg aaaaaaaccc tctcttcctg
     4381 ctttgcccat ctgatcgcag ctggcctgat cgccgccggc ctgctcacgg gatgcggcaa
     4441 cccgggtaac acctccgaca aggataccac ggcaacggcc gccgcagccg acacgatcga
     4501 cgtccacaca gactggaaga tcggcgtcca gctgtggacc tttcattatg tccccttcgt
     4561 acaggcgatc gaaaaggccg atagcgccca tgtcaaatac ctcgaagcta tccccggtca
     4621 acccctgggc ggcggcatga aggactcttt cggtatcgac atgtcggatg ccagcaaagc
     4681 gaagatcaaa aagctgctgg gatccaaagg catgcagatc gtggcggtgg gcgtcatcgg
     4741 gccctccagc gtcgccgaat ggaaacgctg gttcgatctc gcaaaagaat tcggtctcag
     4801 ctatatcacc tgcgaaccgc tcagagaaca ctggaatgcc atcgatagcc tcgccggtat
     4861 ttacgggatc aatatcgcca tccatgatca tcctaaaccc gatccgtact ggcaccccga
     4921 ttccgtgctg gccgctatcg ccgggcacaa gcatatcggc gcctgtgcag acgtcggtca
     4981 ctggacccgc aacggtctcg accccgtcga ctgtctcaaa aaactggccg gacacatcta
     5041 tggcgtccat ttgaaagacg tcgattccgc cgggaatgtc aatgcgaatg acaaggtcgt
     5101 gggcacgggt gttatttctt ttccaccgat tttccaggaa ctgaaacgtc agcgtttcaa
     5161 agggatgttc tctatcgaac gcgaaggcaa ctggtataac aatgtacccg atgtcactgc
     5221 gacgataaga ttttataacg agcagctgag caaattatga tcggtaaaaa ccgaagcggg
     5281 atttttgttg tccaaagcat aaaaaaggcc gccccaacgg ggcggccttt gaatttgtct
     5341 ttcgatgcct gatcaataac caggattctg ctgacttgca atattggcat tcgcctggat
     5401 ctcgttcagc gggatcggat agaccctgcg atagtcggag tagggcatgg tcagcggacc
     5461 attgatggaa cccgaattga tcgccggccg gacgatatcc atcttcagac ggttcagatc
     5521 gtagtagcgg tctccttcga aggccagttc cttccttctc tccgtcagga tatcgctgcg
     5581 cagctgcgcg ccggtggaag aatatcccgc gaaagccgga tcgcggactg ccatcagatc
     5641 gttcagatac atccgtgcat ctgcctcgtt gcccggcagc gacgcttccg cggcgatgag
     5701 atacacttcg gccagacgga tcactttggg attgtcctta tcggtgctcg tatagttggg
     5761 atatttcccg acaagagtag ccgtcgctcc ggactttgtc gtgcccggta ccagcacctg
     5821 gttccggaca tcggtagcgc tgtacaggtt aaccaggtcc tgcgaggcat agatatccgt
     5881 gtaaccgccc atgtacatgg catcgatacc gtcggtacca ttgttgttca cggcgtcggc
     5941 gtcgacctca tacaacactt ccactttatc gctcttcctg gccggattgc tccagaaagc
     6001 cgcgtaagct ccggcggtct gcgccagcgt gaacccgcca ttgttgatga catctacggc
     6061 cgcagccttc gcattcgtat tgtcacccat atacagatag gtgcgggcca acagggcttc
     6121 cgcggcatac ttgctgatca gaccggaatt gctgtaagcc ggcgcattct gaaatgccgc
     6181 cttgaggtcg ctgactattt gggcatacac cttcccgacg gaatcccttt tggggagcag
     6241 tgaaggctga tagtgcagga tcagcggtac gcccagcgcg ttgggattat ccgtataagg
     6301 ccgcgcatac atattgacga gcttaaaata cagcagcccg cggagagcat aggcctgggc
     6361 cttgattgct tccgctcctg tcacctctgc atcgatgacc tggttggctt cctgtatccc
     6421 ctggtaggcg gcattccagt cgccttccac gacgccatcg gcgttcgtaa aggtgtattt
     6481 gaactggttg agataccggc cggaattgtc cacttctacg aaggtattgt cggcctgcag
     6541 gtcgccgatg acgggcatat cccttccata gagcgcaacg gtccgcatct gggcataggc
     6601 gccattgaga gccgtttgca gctgcgagac ggaacccagc gcttcgctta acgtggtgct
     6661 gctataggga gatttgttga cgaaattctt tgaacaggag gaaaggaaga aaagaccgat
     6721 ccccgcgaat aatatatatc tttttctgtt gttcatgata attgcttttt tcgatttagg
     6781 actagaaacc taatttaatg ccggcggtga tggccttcgg cgtgaatacc tggaaatcgg
     6841 cgagactgtt gactcccagc tcaggatcga tagccaggtt cttgtccgtg gcgaaggtga
     6901 gcaggttggt gccccgtaca tagatcatta tattattgat atgcgccctg gacatcgtgc
     6961 tcttcggcag cgaataagcc agctcgatat tccgcagacg gatatagttc cctttataga
     7021 gataccgggt ggacggaacg ttcgagttct tgtttccgcc taatacttgt ctcggcacat
     7081 tcgtgatctg accggcatgc gtccagccgt tgagctcgtt gctcagctga ccgaaataag
     7141 gtagatagag accatcgctc tggagatagt tctcccaggt cgaatagaca tagttgccga
     7201 aattgtagta caattgagcg gtaagcgaca gtcctttata ctggaatgtg ttggtccagg
     7261 cgccgaaata ctgcggcgtg ccaagcttgc cggtgagcga aagcttcacc tggttggacg
     7321 tgctggtgac cgttttgcgc gtagcatccg taaaccattg cgggtcgccg ttggctgggt
     7381 tggcgccgtc ccaaagccgc gtatagaact cgtgcatgtc atgaccctcg gtgatattgt
     7441 agcgggtatt ctgcgagatc ggggagtgat tgtacagctc cgtgacccgg ttcttgttat
     7501 gcgagaaatt gatggtcgac gtccaggtga aatccttcgt caggatgggt ttgcccgtca
     7561 gcgtgatctc gataccctgg ttgtacatgc tgcctacatt ctgcagctgg gtggaacccg
     7621 ggacgccggc ggtggcggaa aggggcacca atgccagcaa ttgtgaggtc gtccgtttgt
     7681 aataatcgac cgaaccgccc agcctgtcct tccagatacc ccaatccaac ccgacgttga
     7741 aggatttgtt aagctcccag gtgaggttgg ggttgccgac attattgagc gtgctacccg
     7801 cattgccctc gtaagggttg ccatagccat attgagcgag cgctgcataa tcgtcgaacc
     7861 cattggtatt acccgctgta ccataggagg cccgcagctt cagcagattg aacccggaca
     7921 tgcctttcat gaactgctct tcattgatgt tccaggaacc gcccacggac cagaagctgc
     7981 tccatttatg gccctgtgca aatttggaag atccgtccag ccggtagctg cccgaaagga
     8041 tataccggtc ctggtagtta aacaccgcct gcgagaaata ggagttcagc gagttgctga
     8101 ccggcaggtt ataggcgctg accggagcgg ccgccgaggc cagataggtt agcagggtgg
     8161 tgctcgggaa gttctggccc tgcgcctgga tcgaataatc gttcaggtta taggcttcgg
     8221 taccggccag caggtcaaga tagaagtcct tgtcctggtt caggtttgcc cggtagttga
     8281 cctggtttgt ccaggtccag tcgaagatct tccggtcggt ggcgatacca taacccagcg
     8341 ggtaaccgtc cccatagatg gggttccagt aaaggtattc gttggcatct aaaaattctg
     8401 cgctgtactt gctggtgaat ttcaggttgt ccaggatctg gtattctccg gagacatagc
     8461 ctcttaattc cgtttgccca tcggtatacc ggttgaatgc ggcgatggcc agcggattga
     8521 actgggcgcc ttcggggaac tctccctccg ggtcgttgaa ccgcaagctg ccgtcggcat
     8581 tgtagggcga ataatagggc ggcaggaaga aagcgcccga tacggggctc gcataggccg
     8641 cacttccggg aggagctatc tggtagctgt agctgccgtt gatcccgaca ttgaacagga
     8701 tcttgtcatt caccttattg gtgacgctga cattcccgtt gtacctcttg aaattggtgg
     8761 cgatggtggt gccgtcctgg tcgaaatatc ccgcagatgc gaaatattgc gttttctcgt
     8821 taccgccgct gaggctgaga ttgtactgct gctggttgcc cgtacgcgtt acctggtgca
     8881 gccagtcggt attcgttttc gtccagtccg caggcagccc gagcccgtta ttggggtcga
     8941 tgatgaacgc atccgcctct gcattggtgt tggcataacc gccattgatg gtcgccagat
     9001 ccagcagctg atggtattgc ggggtagtca gcggccggtt gttcttattg gcgaacgcga
     9061 cgctgttctt gcccacttcc gtcgtaaaat tgatccgggt cttgccggct ttgcctttct
     9121 tcgtggtgac gatgatcacg ccattggctg ccctcgaacc ataaagcgcg gtggcggcgg
     9181 catccttcag gacgctgatg ctttcgatgt cgtcaggatt gagaccactc aaggcgttgg
     9241 tgctggtaga ggtcgttgcc aggtcgccgc tggtagcaac ggccccgtcg atcacccaca
     9301 gcgggcttgc gctggcgctg atagaaccga tacccctgat ccggacatcc acggcggccc
     9361 ccgggatgcc attcggcgcg gatatcatca ggccgggagc ggcgccctgc aaggcctggt
     9421 ctaccgaagc cagcggcttg tccgctatct gcgagccatt gatctgcccg accgcgcccg
     9481 tgatattggt ctttttcagt gtgccataag cgacgacgac tacttcgtcg aggttcttgg
     9541 tgcctcgtgc gaggttcacc gtgccaatgc tggtcttgcc cgcgatgtcg atctcctgcg
     9601 tctggaagtt gacagccgag atgaccagcg ttttgtcttt tccggacacg gagataagga
     9661 aggttccgtc attcccggtg gcagtcgccc tgttggtgcc ctttatgccg atggttgccc
     9721 cggcgaccgg ggatcccatc gcatccgtaa ctgtccctga aatggtgtgt tgattttgcg
     9781 ccgcaagttg cccgaatagc agggca
//
LOCUS       DASYSV010000612         8187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1020620, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000612 DASYSV010000000
VERSION     DASYSV010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8187
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020620"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VGQ51_19100"
     CDS             <1..440
                     /locus_tag="VGQ51_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9038756.1"
                     /translation="KLCTTVHMLVRKDKMRASQIMQERVQQTPNIKLYWNSETDEILG
                     ESRVEALRIRNNKTGEKKDIPISGFFVAIGHQPNSGIFADFLDMDESGYIKTVPGTSR
                     TNVEGVFAAGDVQDKIYRQAVTAAGSGCMAALDAERYLSSKGI"
     gene            440..799
                     /locus_tag="VGQ51_19105"
     CDS             440..799
                     /locus_tag="VGQ51_19105"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEV9038757.1"
                     /translation="MVTVQLHKLIFSGRHGVFKEELATGNTFEVNLDVSYEESEHPFT
                     GLDSIVNYVNLFGIIKERMHQPSPLLEKIADEIITKIREHYPFVREVILSIYKLQAPI
                     GNFQGKAGMTLHKKFPA"
     gene            complement(844..2208)
                     /locus_tag="VGQ51_19110"
     CDS             complement(844..2208)
                     /locus_tag="VGQ51_19110"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV9038758.1"
                     /translation="MDLTTNSPIYKVSVTNRDILHMALPISLAILVPQINFITNNIFL
                     GHLDSAGDALSTAGITGVYYLLFAVIGQGLNNGLQALIARRAGEGRVGEIGKLFSQGM
                     FIAFVLAAAGITITYFLAPLALSWSLHSAALRVQAIAFLRIRIWGLPFLYIYQMRNAL
                     LVGTNQSKFLVYGTLAEAVVNICLDYGLIFGHFGLPAMGFNGAAVSSIFAEASGMLVV
                     FFVIHAKGISKHLQLYRHWTFDPANSRLILVQSSPLIFQFTISIAAWTFFYILVEHHG
                     RQALAISNTMRNIFGLFGVFTWAFASTTNAMVSNIIGQGLEDRVTELIRKIVKLSVSF
                     AVIVAILLNCFPGLFLSVYGQDQAFTQAAIPVIRIVSSALVLMSFSTVWLNAVTGTGN
                     TRVNLGIEVITIVLYCIYVYLTLERWNLSIVVGWISEWLYWISLFLLSFTYMRSGAWR
                     GKVI"
     gene            2295..3149
                     /locus_tag="VGQ51_19115"
     CDS             2295..3149
                     /locus_tag="VGQ51_19115"
                     /inference="COORDINATES: protein motif:HMM:NF024271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HEV9038759.1"
                     /translation="MHKPAFQIEVSNISDEELLQSRLLVEVNPNAFTYVLLNQRNMSP
                     LVVKYFQLEQSKDHPLIEVLREIMEEEELLRRTVKDTLLVYNFPESSLIPEPVFTTDN
                     NREIIDILHGNLQKGMILTEKIPWWELFNIYRVSPDLHHLLRQTFSAGKCWHYYSLML
                     KSYKMFDSADKEDCIKVIFYADKMVVLVVRQGRVQLIQTFLYQDTKDVAFYLLNCCRQ
                     LGMNQEGVRLLVGGLIDRQSTLSSELHKYFLQVYFEDIDESIKVTDELKELPLHYFSS
                     ILKMAVCA"
     gene            3140..3679
                     /locus_tag="VGQ51_19120"
     CDS             3140..3679
                     /locus_tag="VGQ51_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEV9038760.1"
                     /translation="MRVIGGTLGGRRIHPPAKMPHTRPTTDIAKEGLFNILENNLDLA
                     SLRTLDLFGGTGSISYELASRGVHDLTVVEKDPAMFAFIQKSSKELGLSEYYKVVRMD
                     VFKYIEQCRETFDFIFAGPPYALTNIDDLPRLAPRLLNDNGWFVLEHTPRNNYEGYPL
                     FVTGRHYGTTIFSIFVRKT"
     gene            3676..4422
                     /gene="bioD"
                     /locus_tag="VGQ51_19125"
     CDS             3676..4422
                     /gene="bioD"
                     /locus_tag="VGQ51_19125"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEV9038761.1"
                     /translation="MKPLFITGIGTGVGKTLVSAIVAEALGACYWKPVQAGYEEGTDT
                     QWIAARLSHPWARVLPEAYKLKLPASPHIAAREEGVVISLEEIESQFHALRSGLPVLP
                     GQSDPRVEGLPGWSAPAAGEYFVIEGAGGLMVPLNDREFMVDLIKKLDARVILVSRNY
                     LGSINHSLLTAAVCKEHGLDIVGWIFNDQYLDYEREICDWSGYRKIGSIPFDARPDKQ
                     FVSEQGKKIRTSLLFQLEMNRTGIPRSASS"
     gene            4458..5528
                     /gene="lpxK"
                     /locus_tag="VGQ51_19130"
     CDS             4458..5528
                     /gene="lpxK"
                     /locus_tag="VGQ51_19130"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEV9038762.1"
                     /translation="MNFNFPLLKPIRILLFPFSLLYAAVVKVRNWCFDKKIFHSTEFN
                     LPIICVGNLAVGGTGKSPMVEQLVRLLKDDYPVAVLSRGYKRKTKGYALADDKTSALD
                     IGDEPMQFHRKFPEVPIAVGEERIVAIPQLLHDRPDTRVIILDDAFQHRAVRAGLNIL
                     LTDYSNLFTRDWWLPSGDLRDAPSSYKRADIIIVTKCPDQLSEDERQSLAMEIGPREH
                     QQLFFSAIRYGAPYHILRHGATTVIDADVEVLLVSGIANPGPLKRWLNEHSRTYYEMA
                     YGDHHIFTIDDLKAVMRRFEGIAAEKKIIVTTEKDAVRLVKFRQELGQCPMYVLPITP
                     LFLFGDDGRFKTEIFKFINAFQ"
     gene            5525..7654
                     /gene="rnr"
                     /locus_tag="VGQ51_19135"
     CDS             5525..7654
                     /gene="rnr"
                     /locus_tag="VGQ51_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV9038763.1"
                     /translation="MKKKKKINRKKDHAAGASQYRGRLDVTRSGMGFVIVEGRDQDIL
                     VRPADFNTALHGDTVRVRVTGDGARNGRLQGEVTEVVDRKQLEFMGHIEISPSFAFFV
                     AETDKPMPDIYIPLTALNGAANNAKVIVRIKEWEKNKKPQGEVIQVMDSSDVNDQAMK
                     EILLENGFPIFFPENVLEESERLPDLIPSAEIAKRKDMRPVLTFTIDPVDARDFDDAL
                     SIRHLDNGQLEIGVHIADVGYYVEPETALDKEAYTRATSVYLPDRVNPMLPERISNEL
                     CSLRPHEDKLTFSAVFRMTPKGEVKDVWIGRTVIHSDHRFTYEEVQEIIDQKTGSYQS
                     GIELLHTIAQRLRKNRFRKGAINFSSTEVRFRLDEKGKPIGIVVKESKESHQLIEEFM
                     LLANRTVAETVGKVKVNKKPVPFPYRVHDTPDKEKLLPFIEFAKKYGHVFDITSPQAI
                     ARSFNQMLVDVQGKPEQHVLEQLGIRTMAKAIYTTENIGHYGLGFEHYCHFTSPIRRY
                     PDVLVHRILQECLDGNVQPDKKLEAKCKQSSERERAAMDSERAANKYKQVEYMQGFLG
                     EEFEGVISGVASFGFWVETIEHKCEGLVSINSLLSYDDFRHVESDYSLVGRRSGRQFR
                     MGDKVTIKVISANLSKRQLDYEWVIAVGEKEEEGFGAKPARKPGGGQEHKQSERRHHK
                     TAGGKHRRSGGGETHKAQGKQGRKKKK"
     gene            7669..>8187
                     /locus_tag="VGQ51_19140"
     CDS             7669..>8187
                     /locus_tag="VGQ51_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV9038764.1"
                     /translation="MHSFEELTADFAVRFNERHFPLQPASLYDPADYFLGLGGKRIRP
                     VLCLMGNELFGIIEEDAWPMAIALELFHNFTLIHDDIMDKAPLRRGMPTVHAKYSIPT
                     AILAGDVMMVGGYRYISRIRSPHIRQILELYNATAVSVCEGQQLDMDFESRDRVTMDE
                     YLHMIELKTSVLL"
BASE COUNT         2008 a         2059 c         2382 g         1738 t
ORIGIN      
        1 ccaagctgtg cacgacggta catatgcttg tccgcaagga taaaatgcgc gcgtcgcaga
       61 tcatgcagga gcgtgtgcag caaaccccga acatcaagct ctactggaac agcgagacgg
      121 acgagatact gggggaaagc cgggtggagg cgttgcgcat ccggaacaac aagacgggtg
      181 aaaaaaagga tatcccgatc agcggctttt ttgtggcgat cggtcatcag ccgaactccg
      241 gcattttcgc ggatttcctg gacatggatg aaagcggcta tataaagacg gtgcccggca
      301 ccagccggac caatgtggaa ggggtatttg cggcgggcga tgtgcaggac aagatctacc
      361 gtcaggcggt gacagcggcc ggcagcggtt gcatggccgc cctggatgcg gaaagatatc
      421 tctcttcgaa aggaatctaa tggtaacggt acaattacat aaactcattt tttcgggccg
      481 tcacggcgtg ttcaaggaag aactggcgac gggcaatacg ttcgaggtga atctcgacgt
      541 gtcttatgag gaaagcgaac atccttttac cggtctcgat tcgatcgtca actatgtgaa
      601 cctcttcggc atcatcaagg aacggatgca tcagccgtca ccccttctcg aaaagatcgc
      661 cgacgagatc attacgaaga tcagggaaca ttatcccttc gtccgggagg tcatcctgtc
      721 catttataaa ttgcaggcgc cgatcgggaa ttttcagggc aaggcgggaa tgacgctgca
      781 taaaaagttc ccggcctgag cgatccgtga aatggccgat ccgtgaaatg gccgatccgc
      841 ggctcagatc acttttcccc tccaggctcc gctacgcata taggtgaagg agagaaggaa
      901 gagcgagatc caatacagcc attcggatat ccaccccact acaatggaca ggttccagcg
      961 ctcgagggtc agatagacgt agatacagta gagcacgatc gtgatgacct ctatccccag
     1021 gttaacccgc gtgttgcccg tgcctgtcac cgcattcagc caaacggtgg agaacgacat
     1081 cagcaccagc gccgaggata cgatccggat gacgggtata gcggcctgcg taaacgcctg
     1141 gtcctgtccg tacacggaaa ggaacagacc cggaaagcag ttgagcagga tggcgacgat
     1201 aacggcgaag gatacgctga gctttacgat cttccggatg agctctgtaa ccctgtcttc
     1261 aagcccctgg ccgatgatat tgctgaccat ggcatttgta gtggaggcga atgcccaggt
     1321 aaagacaccg aaaaggccga agatattccg catggtattg ctgatagcca gtgcctggcg
     1381 gccatggtgt tcgacgagaa tatagaagaa cgtccaggcc gcgatgctga tggtgaactg
     1441 aaagatgagc ggcgaggact ggaccaggat cagacggctg ttggccggat cgaatgtcca
     1501 gtgacgataa agctgaaggt gtttgctgat accttttgcg tggatcacaa agaacaccac
     1561 aagcataccg gatgcttcgg cgaagatgga ggatacggcg gcaccgttga accccatagc
     1621 agggagaccg aaatgtccga agatcaaccc gtaatcgaga cagatgttga cgacggcctc
     1681 tgccagcgta ccatagacca ggaacttact ctggttggtg cccacgagca gagcattgcg
     1741 catctggtaa atatagagga agggcaaacc ccagatgcgg atgcgcagga aggcgatggc
     1801 ctggacccgg agcgcagcgg aatgcaatga ccaggaaagg gccagcggcg cgaggaaata
     1861 ggtgatcgtg atacccgccg ctgccagcac gaacgcgatg aacataccct gtgagaacag
     1921 cttcccgatc tcgccgacgc gtccttcccc ggcacgacgt gcgatcagcg cctgcagacc
     1981 attgttgaga ccctgtccga tgacggcgaa cagcagatag tagacgcccg tgatgccggc
     2041 tgtagaaagg gcgtcacccg cggaatcgag atgccccagg aaaatattgt tggtgatgaa
     2101 attgatctgg ggcacgagga ttgccaggct gatgggaaga gccatgtgaa ggatatcccg
     2161 gttggtgacc gatactttat agatcgggct gtttgtcgtc aggtccattg catgaatgtg
     2221 ggccgatgcc ggcggccttt gaaaaagagg gcaatttaca ccgttttgta gtattattgc
     2281 attaccggaa agttatgcat aaaccagcct tccagatcga agtctcgaat atctctgatg
     2341 aggaattgct acaatcccgc ctgttggtgg aggtgaatcc caacgcattt acttatgtat
     2401 tgctgaacca gcggaatatg agtccactgg tggtcaaata tttccagctg gagcagagca
     2461 aggaccaccc gctgatcgaa gtgctgcgcg agatcatgga agaagaagag ttgttacgtc
     2521 gcaccgtgaa ggatacactg ctggtgtaca attttccgga aagcagtctt atccccgagc
     2581 cggtcttcac gacggataac aaccgcgaga tcatcgacat cctccatggc aatctgcaga
     2641 aaggaatgat cctgaccgaa aagatcccct ggtgggagct attcaatatc taccgcgtct
     2701 cgccggacct gcatcatctg ctgcggcaga ccttttcggc cggtaaatgc tggcattatt
     2761 acagcctgat gctgaagagc tataagatgt tcgattcggc ggacaaggaa gactgcatca
     2821 aggtcatctt ttatgccgat aagatggttg tgctggtggt ccgccagggc cgggtccagc
     2881 tgatccagac ctttctctac caggatacca aagacgtggc cttttatctg ctgaactgtt
     2941 gccggcaact gggtatgaac caggaagggg tgcggctgct ggtaggcggt ctgatagacc
     3001 gtcaatctac gctgagcagc gagctgcaca aatatttcct gcaggtatat tttgaagaca
     3061 tcgatgaaag catcaaggtc accgacgagc tcaaggaact gccgctgcat tatttttctt
     3121 cgatcttgaa aatggccgta tgcgcgtgat cggcgggaca ctgggtggaa gacggataca
     3181 tccgccggcc aaaatgccgc atacacggcc gacgaccgat attgccaagg aagggctgtt
     3241 caacatcctg gagaacaatc ttgacctggc ttcacttcgg accctagatc ttttcggggg
     3301 tacgggcagc atcagctatg agctggcctc gcgcggcgtc catgacctga cggtcgtcga
     3361 aaaggacccc gcgatgttcg cctttattca aaagagctcg aaagagctgg ggttatcgga
     3421 atactacaaa gtggtccgga tggatgtctt caaatacatt gagcaatgcc gcgagacctt
     3481 cgatttcatc ttcgccggtc ccccgtatgc gctgaccaat atcgacgatt tgccgcggct
     3541 ggcgccgcgt cttttgaacg ataacgggtg gttcgtgctg gaacatacgc cgcgcaataa
     3601 ctacgaggga tatccgctgt tcgtgaccgg gcggcattac gggacgacca ttttctcgat
     3661 ctttgtccga aaaacatgaa acccctcttc ataacaggta tcggtaccgg tgtcggcaag
     3721 accctggttt cggccatcgt cgcggaggcg ctgggggcct gctattggaa acccgtgcag
     3781 gcgggatatg aggaagggac ggatacgcag tggatcgccg cgaggcttag ccacccctgg
     3841 gcaagagtgt tgccggaagc gtacaaactt aagttgccgg cttccccgca tattgccgcc
     3901 cgtgaggaag gggtcgtcat cagcctggag gagatcgaat cgcaatttca tgctttacgg
     3961 tccggtcttc cggtactgcc cgggcagagc gatccccggg tggaggggct tccgggttgg
     4021 tccgcgcctg ctgccggcga gtattttgtt atcgaaggag cgggtggctt aatggtaccg
     4081 ctgaacgacc gggagtttat ggtcgatctc ataaaaaaac tggatgcgcg ggtgatcctg
     4141 gtgagccgca attatctggg cagcatcaat cattcgctgc tgaccgcggc cgtctgtaaa
     4201 gagcatggcc tggacatcgt gggctggata ttcaatgatc aataccttga ttatgaacgg
     4261 gagatctgcg attggtccgg ttaccggaag atcggctcca ttcccttcga tgcccggcct
     4321 gacaagcaat tcgtgtcgga acaggggaag aagatccgga cctcgctatt gttccagctt
     4381 gagatgaacc ggaccggcat accccgaagc gcctcatcct gaatttttta cgtatcttta
     4441 aagggcgaat agccgatatg aatttcaact tcccgctgct caaacccata cgaattttac
     4501 tgttcccttt ttcattgctt tacgctgccg tagtcaaggt tcgcaactgg tgtttcgata
     4561 aaaagatctt tcattccacc gaattcaacc ttcctatcat atgcgtcggc aacctggcgg
     4621 tcggcgggac agggaaatcg ccgatggtgg aacaattggt gcggttgttg aaggacgatt
     4681 atcccgtcgc cgtgctgagc cgcggctaca aacggaagac gaaggggtat gcgctggccg
     4741 atgacaagac gtccgcgctg gatatcggag atgaaccgat gcagttccac cgcaaattcc
     4801 ccgaagtccc gattgcggtg ggggaagaaa ggattgttgc gatccctcaa ttgctgcatg
     4861 accggccgga cacccgcgtc attatcctcg atgacgcgtt tcagcaccgg gcggtccggg
     4921 ccggtctcaa tatcctgctg acggactatt caaacctgtt cacccgcgat tggtggctgc
     4981 ccagcgggga tctgagggat gcgccttcat cgtacaaacg ggccgatatc atcatcgtta
     5041 ccaaatgccc cgaccagttg tcggaggatg agcggcaatc gctcgcgatg gagatcggtc
     5101 cgcgggaaca ccagcagctg ttcttttcgg cgatccgcta cggggcacct tatcatattc
     5161 tccggcatgg ggccaccacc gtcatcgacg ccgacgtgga agtgctgctc gtcagtggga
     5221 tcgccaaccc gggcccgctg aaaagatggt tgaacgagca ctcccggacc tattatgaga
     5281 tggcctatgg cgatcatcat attttcacga tcgatgatct gaaagccgtg atgcggcgtt
     5341 tcgagggtat tgccgccgaa aagaagatca tcgttaccac cgaaaaggat gcggtgcggc
     5401 tggtaaagtt cagacaggag ctcgggcaat gcccgatgta cgtgcttccg ataacgcccc
     5461 tgttcctatt cggggatgac gggcggttca agacggaaat ttttaaattt atcaatgcat
     5521 ttcaatgaag aaaaaaaaga aaattaatag aaagaaagac catgcggccg gagcctccca
     5581 gtatagggga cggttggatg ttacgcggag cggcatgggc ttcgtgatcg tagagggccg
     5641 ggatcaggat atattggtcc gtccggcgga cttcaatacc gcgttgcacg gggacaccgt
     5701 gagggtacgc gtcacgggcg atggcgcccg gaacggaagg ctgcagggag aagtcacgga
     5761 ggtcgtcgac cgcaaacagc tggaattcat ggggcatatc gagatcagtc cctccttcgc
     5821 cttttttgtc gcggagacag acaagccgat gcccgatatc tatatccctt tgaccgcgct
     5881 gaacggggcc gccaacaatg ccaaggtcat cgtccggata aaggaatggg agaagaataa
     5941 gaaaccgcag ggcgaggtca tccaggtcat ggacagcagc gatgtgaacg accaggcgat
     6001 gaaggagatc ttgctggaga acgggttccc gatctttttc cctgaaaacg tgctcgagga
     6061 atcggagcgt ctgccggatc tgataccatc cgcagagatc gcaaaacgga aggacatgcg
     6121 ccctgtgctg accttcacca tcgacccggt ggatgcacgg gatttcgatg acgcgttatc
     6181 tatacgacat ttggataacg ggcaactgga gatcggggtc catatcgcgg atgtcggcta
     6241 ctatgtggag ccggagaccg ctctggataa ggaagcctat acccgtgcca cttcggtcta
     6301 tctgcccgac agggtgaacc cgatgctgcc cgaaaggatc tcgaatgagc tgtgctcgct
     6361 gcgtccgcat gaggataagc tgaccttttc cgccgtattc cgcatgacgc cgaaaggaga
     6421 ggtgaaggat gtttggattg gcaggacggt gatccactcc gatcatcggt tcacgtatga
     6481 agaggtacag gagatcatcg atcagaaaac gggcagctat cagtccggga tcgagctgtt
     6541 gcataccatc gcccagcgtc tccgcaagaa tcgtttccgg aaaggcgcga tcaatttctc
     6601 ttccaccgag gtccgtttcc gtctcgatga gaaaggcaag cctatcggca tcgtagtaaa
     6661 agaaagcaag gaatcgcatc agctgatcga ggagttcatg ctgctggcca accgcaccgt
     6721 ggccgagacc gtgggcaaag tgaaggttaa caaaaagccg gttccatttc cgtatcgcgt
     6781 gcacgatacg cccgataaag agaaactcct tccttttatc gagttcgcca agaaatacgg
     6841 gcatgtgttc gatattacgt caccacaggc gatagcccga tcctttaacc aaatgctggt
     6901 ggacgttcag ggcaaacccg aacagcacgt gctcgagcag ttgggcatcc ggaccatggc
     6961 caaggcgatc tatacgacgg agaatatcgg ccactatgga ctgggtttcg aacactactg
     7021 tcattttact tctccgatcc gccggtatcc cgacgtgctg gtccatcgca tcctgcagga
     7081 atgtctggat ggaaacgtcc agccggataa gaagcttgag gctaaatgca aacagtccag
     7141 cgaacgggaa agggccgcga tggatagtga aagggcggcg aataaatata agcaggtcga
     7201 gtacatgcag ggtttcctcg gtgaagagtt cgaaggggtg atcagtggtg tcgcctcttt
     7261 tggattctgg gtagagacca tcgagcataa atgcgagggc ctggtgagca tcaacagttt
     7321 gcttagttat gatgatttcc ggcatgtgga aagcgattac agcctggtgg gccgtcgcag
     7381 cggccggcaa ttccgtatgg gcgataaagt cacgatcaag gtcatctcag ccaatctgtc
     7441 caagcgccag ctggattatg agtgggtgat cgcagtgggt gaaaaagagg aggaaggatt
     7501 cggcgcgaaa cccgcgcgaa agcccggcgg tggccaggag cataaacaaa gcgaacgccg
     7561 gcatcacaaa accgccggcg gcaaacaccg cagatccggc ggcggcgaaa cacataaggc
     7621 acaggggaag cagggaagga agaaaaagaa ataaatgtac atttgaacat gcacagtttt
     7681 gaagaattga ccgctgactt tgccgtccgg ttcaatgagc gtcattttcc cttgcaaccc
     7741 gccagtctgt atgatcccgc cgactatttt cttggtctcg gtggcaaacg gatcagaccg
     7801 gtactatgtc tgatgggaaa tgagcttttc ggcatcatcg aagaggatgc ctggccgatg
     7861 gccattgcgc tggagctgtt tcacaatttt acgctgatac acgacgatat catggacaag
     7921 gctccgctcc ggcggggcat gccgaccgtg catgcgaaat acagtattcc taccgctatc
     7981 cttgcaggag acgtaatgat ggtggggggg taccgttata tcagccgaat ccggtcgccg
     8041 catatccgtc agatactgga gctgtataat gcgacggccg tgagcgtttg tgagggccag
     8101 cagctggata tggatttcga atcccgcgac agggtgacga tggacgagta tttacatatg
     8161 atcgagctga agacttccgt ccttctt
//
LOCUS       DASYSV010000613         6364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_633695, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000613 DASYSV010000000
VERSION     DASYSV010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633695"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VGQ51_19145"
     CDS             complement(<1..373)
                     /locus_tag="VGQ51_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038765.1"
                     /translation="MSLFNLLRIALKALQRNKLRAFLTMLGIIIGVASVIAMVAIGQG
                     SKQSIQSQLSGMGSNMIIIRPNSNISAGARLDATNVQTLSLDDVKALQNQAPDLSAVS
                     PMANSRGQAIFGNNNWPTTIQG"
     gene            complement(406..1155)
                     /locus_tag="VGQ51_19150"
     CDS             complement(406..1155)
                     /locus_tag="VGQ51_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV9038766.1"
                     /translation="MSEQKILELHDIKREFRMGAEIVRALKGVTFEVEAGEFLTIMGS
                     SGSGKTTLLNTLGCLDKPSAGTYLLDGVDVKKLSRNELAKLRNQKIGFVFQAYNLLPR
                     TSALENVELPLLYNPLISARERKERAIKALEAVNLGDRLDHTPSQLSGGQQQRVAIAR
                     ALVNEPVMILADEATGNLDSRSSYSIMALLQDLNQQGKTIVFVTHEPDIAAFSSRTIM
                     LKDGKVMKDSLNENRKSAKEVLASLPKSEDY"
     gene            complement(1199..2536)
                     /locus_tag="VGQ51_19155"
     CDS             complement(1199..2536)
                     /locus_tag="VGQ51_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV9038767.1"
                     /translation="MKRAIIIIVLLLLAGAAAWFFYFRKEEKPIVLQTERPRYGYISK
                     SVTATGTVQPVDTVAVGSQVSGTIRALYASYNDKVRKGELLAELDKSLFMAQVDQFKA
                     NLEVVKAAAVYQKSTFDRQTLLYNTGAISKQDFENSQNQYLAAKAQVESVQAQLAGAT
                     KNLDYASIYSPVDGVIMTKNVSVGQTVAASFNTPTLFIIAKDITKMQVQAAVSEADIG
                     DVKLGLRATFTVDAYPDMSFSGTVNQVRLEPVVSANVVTYSTIITAPNEDLRLKPGMT
                     ANIFIFTREIDSALLISAKDLKYKPDVSLSKQYVIFPDTVDEREVQVEAKRSGNNPLS
                     PPRHRHDSSSRVIDSAPQTGTPAFVWIKKGDSLIEKKITTGLNNDTKVQVLSGLDPDD
                     EVVGGIETQTAGTASGGGVRSPFMPARRGGNRPSGGSRPAGGGGGGGRGGRGS"
     gene            complement(2539..3867)
                     /locus_tag="VGQ51_19160"
     CDS             complement(2539..3867)
                     /locus_tag="VGQ51_19160"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV9038768.1"
                     /translation="MSNRRSVFILLLAFLTGTAHAQDSTAGRPATLWTLQSCLDYAKK
                     YNIQLNSLRLTQKTAEQNYQLSKAARQPNLNGGVGLTYTHNGSAAVNSFQQSSFNNNF
                     SLSSGLTIYNGGYISDDIRQKNLEIQSANLNILETENNITLQITQAYLAILLAKENVV
                     YVQDLVNTTAAQVDQGKKEYEAGSIARNAYIELQANLASDKYNLITAQSSVRQNKVTL
                     KQLLQLPFQDSFDIVSPDTIFSKEVVAPLDTAVAIALRTRPEVMNAQLGIDISRLDLA
                     KAKTGYLPLLTASAAIGSAYSSSNSNDYFKQQDNSFFQQAGLNLSIPIFTKRVVKTNV
                     ENAKIEIDQSKLSLLNTQTTLALTIEQAYINVVNAQGQYDAAVEGLKANQESFRIATE
                     QLRVGATNIVDYLQQKTLYTQAFQVYLQAKYNAALSVRIYDFYMGIAVKL"
     gene            complement(4194..4493)
                     /locus_tag="VGQ51_19165"
     CDS             complement(4194..4493)
                     /locus_tag="VGQ51_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038769.1"
                     /translation="MQRVLFAALFSTALLLAGASANAQTHHYVKHHPEERTMTHPNAP
                     SSHHVWVNSEWKWHDGKYEESAGHWAVPPRGHHEWTAGRWEKNSRGDYWVPGHWH"
     gene            complement(4631..5578)
                     /locus_tag="VGQ51_19170"
     CDS             complement(4631..5578)
                     /locus_tag="VGQ51_19170"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HEV9038770.1"
                     /translation="MATNEKLRENQLATEFLNCSDSSGLAGFRKKMEALKWNTALYDK
                     SVASLREIKTPEEMALMRKTVSISAIAHAEVMKAIQPGMSEAELQGLQEYVHKKPGAE
                     YVGYPSIVGAGANGCILHYEDNTRVNIGRNLVLMDVGAEYHGYSADVTRTVPSSGRFT
                     PEQRAVYDLVYQAQEAIFPLCHKGTPFADLNKKATEVLAQGLLRLGIIHNASEVSRYY
                     PHGCSHFLGLDVHDMGNYEQPLRENMVITVEPGLYIPAGSPCDKKWWDIGVRIEDDVR
                     IGADKFELLSWQAPRKSEDVEKLAAQKSAVNGMTLPAVQ"
     gene            complement(5615..6244)
                     /locus_tag="VGQ51_19175"
     CDS             complement(5615..6244)
                     /locus_tag="VGQ51_19175"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEV9038771.1"
                     /translation="MRKPICLLFGLFLAAACFAQEDLPKDYLTPAFHSGRRDALRALM
                     PPRSVAVIFSYPERVFSKDVDYPYHPNPDLYYFSGYKEPDAVLLIFKEPQHAPGGGEY
                     RELFFVQQRDPQAEQWTGRRLGVEKVKSELGFDSVFNGSEFRNYPVDLSSFDHILFAG
                     IPDDVHDDPSDSADLYDVLQAFRAKAALPADFDAQVYDILAILANRSTV"
BASE COUNT         1239 a         1742 c         1793 g         1590 t
ORIGIN      
        1 cgccctgaat ggttgtcggc cagttgttgt tgccaaagat ggcctgtccc ctggaattgg
       61 ccatcggact cacggcgctc agatcaggcg cctgattttg cagggccttc acgtcgtcga
      121 ggctcagcgt ttggacgttg gtggcgtcca gccgggcgcc ggcggagata ttgctattgg
      181 gacggatgat gatcatgttc gaacccattc cggacagctg cgattggatg ctttgcttcg
      241 acccctgccc gatggccacc atggcgatca ccgaggcgac accgatgatg atgcccagca
      301 tggtcaggaa ggcccgcagc ttgttccgct gaagggcttt caatgcaata cggagaagat
      361 tgaaaaggct catgaaggat tcaaattcgc gcttgtgtaa tttacttaat aatcttcgga
      421 ctttggcaga gaggccagca cttctttggc cgattttctg ttctcgttga ggctgtcttt
      481 catgactttg ccatctttta acataatggt cctgctgctg aaggcggcga tgtcgggttc
      541 atgcgtgacg aacacgatcg tcttaccctg ctggttcaga tcctgcagca gcgccataat
      601 gctgtatgag ctccgggaat ccaggttacc cgttgcctca tctgcaagga tcatcaccgg
      661 ctcgtttacc agggccctgg cgatcgccac ccgctgctgc tgaccgcccg aaagctggct
      721 gggagtatga tcgagacgat cccctaaatt tacagcctcc aatgccttta tcgccctttc
      781 ctttctttcc cgggcgctga tcagcggatt atacaatagc ggaagctcca cattttcaag
      841 cgccgaggtc cggggtagca gattataagc ctggaaaaca aaaccgatct tttggttgcg
      901 gagcttggcc agctcgttgc ggctcagctt tttgacatcc acgccatcca gcagataggt
      961 gcctgccgaa ggtttgtcga gacatccgag ggtattcaac agggtggttt tgcccgagcc
     1021 gctgctgccc ataatggtga ggaattctcc cgcctctacc tcgaaggtta cccctttcag
     1081 cgccctgacg atctcggcgc ccatacggaa ttcccgcttg atgtcgtgta gctccagtat
     1141 tttttgctcg ctcatgaatt tggttttgtc ggctgctctt ctcgtcagcc ggccattgtc
     1201 agctgcccct tcctccacga ccgccgccgc ctcctcctcc tgcgggcctg ctaccacccg
     1261 agggccggtt tccgccgcga cgcgccggca taaagggact gcgtacaccg cctcccgatg
     1321 ccgtgccggc tgtctgcgtc tctatccctc cgaccacctc gtcatcggga tccagcccgc
     1381 tcaggacctg aaccttggta tcgttgttca accctgttgt gatctttttc tcgatcaggc
     1441 tgtccccctt ctttatccat acgaaggccg gagtgccggt ctggggagcg ctatcaatga
     1501 ccctgctgga agaatcgtgc cggtgcctgg ggggcgaaag cgggttattg cccgatcgtt
     1561 tcgcttcgac ctgtacctcc cgctcatcga cggtatcggg gaaaatgacg tactgtttgg
     1621 aaagcgagac atccggcttg tatttcaggt ctttggcgga aatgagcagt gcgctgtcga
     1681 tctccctggt gaagatgaag atattcgccg tcatccccgg tttcagccgc aggtcttcgt
     1741 tgggcgcggt gatgatcgtg gaataggtga cgacattagc cgataccacc ggttccagtc
     1801 tcacctggtt gaccgtcccg gaaaaggaca tatcgggata ggcgtccacc gtgaaggtcg
     1861 cgcgtaatcc caactttacg tcaccgatat cggcctcgga aacggccgcc tgcacctgca
     1921 tcttggtgat atccttggcg atgatgaaaa gggtgggcgt attgaagctg gcggccactg
     1981 tctgaccgac gctgacgttc ttggtcatga tgacgccatc caccgggcta tagatgctgg
     2041 catagtcgag gtttttcgtg gccccggcca gttgtgcctg gacgctttcc acctgcgcct
     2101 tggcggcgag atactggttt tgagagtttt cgaaatcctg cttcgagatg gcgcccgtgt
     2161 tgtacaggag ggtctgcctg tcgaaggtgc ttttctggta taccgccgcg gcctttacga
     2221 cttcgagatt ggctttgaac tggtctacct gggccatgaa cagcgactta tccagctcgg
     2281 ccaggagctc gccttttctc actttgtcat tgtagctggc atagagcgcc ctgatggtac
     2341 ccgatacctg tgaacctacc gccaccgtat ccacgggctg caccgtcccc gtcgccgtca
     2401 cgcttttcga gatataccca tagcgcggcc gctccgtttg aagaacgatg ggcttttcct
     2461 cctttctgaa atagaaaaac caagcggctg cgccggccag caggagtagg acaatgatga
     2521 tgatggctcg cttcatattt ataatttaac tgctattccc atgtagaaat cgtagatcct
     2581 gacgctgagc gccgcattgt atttggcctg cagatatacc tggaatgcct gtgtatacaa
     2641 cgtcttctgc tgcagataat ccacgatatt ggttgcgcct acccttagct gctcggtcgc
     2701 gatgcggaag ctttcctggt tggccttcag tccttccacc gcggcatcgt attgtccctg
     2761 tgcattgacc acattgatat aggcctgctc gatcgtcagc gccagcgttg tctgggtgtt
     2821 cagcaggctc agcttcgact ggtcgatctc tattttcgcg ttctctacat tcgtcttgac
     2881 cacccgtttc gtaaatatgg ggatcgagag attgagtccc gcctgctgga agaaactatt
     2941 gtcctgctgt ttgaaatagt cgttggaatt gctgctggaa tatgcgctgc ctatcgccgc
     3001 ggatgccgta aggagaggaa gatagccggt cttcgctttg gccagatcga gacgcgagat
     3061 atcgatgccc agctgcgcgt tcatgacttc cggacgggta cgcaacgcga tggcgaccgc
     3121 ggtatccagg ggcgccacta cttctttgga aaagatggta tccggggata cgatatcgaa
     3181 gctgtcctgg aaaggcagct gcagcaattg ttttagcgtc accttgttct ggcggacgct
     3241 gctctgcgct gtaatcaggt tgtacttgtc ggatgccaga ttggcctgta gctcgatata
     3301 ggcgttgcgc gcgatgcttc cggcctcgta ttcctttttg ccctggtcta cctgcgcggc
     3361 ggtggtattc accaggtcct gtacatacac cacattttcc tttgccagca aaatggccag
     3421 ataggcttgc gtgatttgca gcgtgatgtt gttctcggtc tccaggatgt tcagattggc
     3481 cgattggatc tcgaggttct tttgccggat atcgtcactg atatatcccc cgttgtagat
     3541 ggtcagcccc gaggagaggc tgaaattgtt attgaaggac gattgttgaa agctgttgac
     3601 ggccgcgctt ccgttatgcg tataggtgag accgacaccc ccgttcaggt tgggttgccg
     3661 cgccgccttc gagagctgat agttctgctc ggccgttttt tgggtcaggc gaagactgtt
     3721 gagctggata ttgtactttt tggcgtagtc gaggcaggac tggagcgtcc atagggttgc
     3781 cggccggccg gcagtgctgt cttgcgcgtg ggccgttccg gtcaggaaag ccagcaggag
     3841 aatgaacaca gaacgtcgat tggacataag ttcctggttt aggttacata tgcgtgacag
     3901 ctgttacaaa ggtggggcca tactatggcc ccattcaccc attcctgtaa atatatgtca
     3961 ttgtatataa aaatgcgggt gctttcgccg gcataccggc cggctgagtg tggatacgca
     4021 ggctgtggaa tttgcgcaac ggttcccctg aggcgggcaa accagtcgcg gccggtaacc
     4081 gccggcttcg gttgagggtt gccgtcattg ccccacggag ggccatttga gcaaagcccg
     4141 gaatctctta cgaaaaattc cgggcgttct gcatctggga gatgatcatc gcatcaatgc
     4201 cagtgtcccg gaacccagta atcgcccctg ctgttcttct cccaacgacc ggcggtccat
     4261 tcatggtgtc cgcgcggagg caccgcccaa tggccggcgc tctcctcgta cttgccatcg
     4321 tgccatttcc attccgaatt gacccataca tgatgggacg aaggagcatt gggatgtgtc
     4381 atggtccttt cttccgggtg gtgcttcaca taatggtgtg tctgggcatt ggcggaagcg
     4441 cctgccagta gcagcgcagt gctgaaaagc gcggcaaaca atacacgttg cataatagta
     4501 tttttatatc ggttatgcca aacccgtgcc gatctgcctg gttggaccgc cggaagcgtc
     4561 gtcatcgcac cgtgggaagg gtcatcatcg catagccgga agcatcgtca tcgcaccgcc
     4621 ggaaaggtcg tcattgcacc gccggcaggg tcatcccgtt gacggcgctt ttctgcgccg
     4681 ccagtttttc tacatcttct gactttcggg gagcctgcca ggacagcagt tcgaatttat
     4741 ccgcgccgat acgcacgtca tcttctatcc ggacgccgat gtcccaccat tttttatcac
     4801 agggactgcc ggcggggata tagaggcccg gttcgacggt gatcaccata ttctcccgca
     4861 agggctgctc gtagttaccc atgtcgtgta cgtccagacc gagaaagtgc gaacacccat
     4921 gcgggtagta acgggacacc tcggacgcat tgtggatgat gcccagccga agcaggcctt
     4981 gcgcaaggac ttccgtggcc ttcttgttga gatcggcgaa aggcgtccct ttatgacaga
     5041 gcgggaagat cgcctcctgc gcctggtaga cgagatcata gacggccctt tgttcgggtg
     5101 taaatctccc ggaagacgga acggttcggg taacatcggc ggaatagcca tggtattcgg
     5161 cgcccacgtc catcaggacg agattacgac cgatattgac gcgggtgttg tcttcataat
     5221 gcaggataca gccgttggcg ccggcgccga ctatcgaagg atatccgaca tattccgcac
     5281 ccggtttctt gtgtacatat tcctgcagac cctgcagctc ggcctccgac attcccggct
     5341 gaatggcctt catcacttcg gcatgcgcga tagcggagat gctaacggtc ttgcgcatca
     5401 gcgccatctc ttccggcgtc ttgatctcgc gaagcgatgc caccgatttg tcatacagtg
     5461 cggtattcca tttcaaagcc tccattttct tccggaaacc ggcgagaccc gacgaatcgc
     5521 tgcaattcag gaattctgtg gccagttggt tttcgcggag cttttcgttc gtggccatcc
     5581 gcaatttcaa ccattgtttg ccctggggca gcatttatac ggtagacctg ttcgccagta
     5641 tcgccaggat atcatatacc tgcgcatcga aatcggcggg aagcgccgct tttgcccgga
     5701 atgcctgcag cacgtcgtag agatcggcgg agtccgacgg atcgtcgtgg acgtcgtcgg
     5761 gaatgccggc gaagaggata tggtcgaagg aggagaggtc gacgggatag ttcctgaatt
     5821 cgctgccgtt gaatacgctg tcgaaaccca gctcgctttt cactttttcc actcccagac
     5881 ggcgtcccgt ccattgttcc gcctgcgggt cgcgttgctg tacgaagaac agctcgcggt
     5941 attcgcctcc gcccggcgcg tgttgcggtt ctttgaagat gagcaggacg gcgtccggtt
     6001 ccttataacc cgagaaatag tagagatcgg gattcgggtg atagggatag tcgacgtctt
     6061 tcgaaaagac gcgttccgga taggagaaga taacggccac cgaacgagga ggcatcagcg
     6121 cccgaagggc atcgcgtctt ccgctgtgga atgcgggagt gaggtagtct ttgggaaggt
     6181 cttcctgtgc gaaacaggcg gcagccagga acaggccgaa cagcaggcaa ataggttttc
     6241 tcatatataa gtttctgcta ctgaagatag ccccctgcgg ctattttctg tcgccgcttg
     6301 tcaaaagggg gttttttacg ggcaccgacg cgttaatttt tataaaaaac ggatcaccgg
     6361 ccca
//
LOCUS       DASYSV010000614         1919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1151430, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000614 DASYSV010000000
VERSION     DASYSV010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151430"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VGQ51_19180"
     CDS             complement(<1..923)
                     /locus_tag="VGQ51_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEV9038772.1"
                     /translation="MPDDRQQHTFDAIVIGSGISGGWAAKELCELGLKTLVLERGRNV
                     VHNKDYPTATMSPWEFPHRKEMPRKFVDENPLITTAAGFGEDTAHFFIKDKDHPYVQE
                     KPFDWIRGYQVGGKSLIWGRACPRWSHYEFTAPARYGYGIEWPITYDEIAPWYSHVER
                     FAGICGNKDGLESMPDGDYLPAFDMNCAEKHLQKKISDWRPDRHMIAGRWAQLTKPSD
                     IHLQQGRAQCQARDMCMRGCPFGGYFSSNSSTIPWAERTGNLTIRPFSVVHSIIYDEQ
                     KGKATGVRIIDTNTKQATEYFARVIFVNASA"
     gene            complement(913..1575)
                     /locus_tag="VGQ51_19185"
     CDS             complement(913..1575)
                     /locus_tag="VGQ51_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEV9038773.1"
                     /translation="MNYKLLLIATLLPSLFAGAPPTPPRRVQQLFNGRDLTGWKHVGP
                     GSMSVENGLLRGHGGMGLLYWAGGPFSNCSFHLVFKMRDENDNSGVFIRIPIEPREEW
                     MPVHYGYEVQIDNHPEKSNEDDYHITGTLYSLTKPLAKPGKPGPEWNTMDITLDGPRT
                     IVYVNGVKVTDYREGEPVPTRKFDFEPQRGRRPDKGWFGIQNHSDNDIVYFKEISVSY
                     AR"
     gene            1665..>1919
                     /locus_tag="VGQ51_19190"
     CDS             1665..>1919
                     /locus_tag="VGQ51_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038774.1"
                     /translation="MKPAPDQSDHSEAAAEQKGGSASRHSGIAAESGVTGSRRLFVKQ
                     GLRAGLITLTGGAVLAACSGGKGEEKEVSPPEDLMQEHGLL"
BASE COUNT          407 a          532 c          538 g          442 t
ORIGIN      
        1 attgcggagg cgttgacgaa gatgacgcgc gcaaaatatt cggtcgcctg tttggtattc
       61 gtatcgatga tccgaacgcc ggtggctttc cctttttgtt cgtcataaat gatagagtgg
      121 acgacagaga aggggcggat cgtcaggttg ccggtacgct cggcccaggg gatggtagag
      181 gaattgctgc tgaaatatcc accgaagggg cagccgcgca tacacatatc gcgagcctgg
      241 cattgcgccc gcccctgttg aagatggata tccgacggtt tggtgagctg cgcccaccgg
      301 ccggcaatca tgtgccggtc gggtcgccag tcgctaatct ttttttgcag gtgcttctcc
      361 gcgcagttca tatcgaatgc cggcaaatag tccccgtccg gcatcgattc cagaccatcc
      421 ttattaccgc agatgcccgc gaaccgctcg acatgcgaat accagggagc gatctcgtca
      481 taggtgatag gccattcgat gccatagcca taacgcgcgg gggcagtaaa ttcatagtga
      541 ctccacctgg ggcaggccct tccccagatg agcgatttgc cgcctacctg gtagccgcgg
      601 atccagtcga acggcttttc ctggacatag ggatgatcct tgtcctttat aaagaaatgc
      661 gccgtgtctt caccgaaacc ggctgccgta gtgatcagcg ggttctcgtc cacgaatttc
      721 cgcggcattt ctttccgatg cgggaattcc cagggagaca tcgtggcggt gggatagtct
      781 ttattatgca cgacattgcg tcccctttcc agcaccaggg tcttcagacc cagctcgcac
      841 aattctttgg cagcccagcc cccgctgatc cccgatccga tcacgatggc atcgaaggta
      901 tgttgctgac gatcatctgg catagctaac tgaaatttct ttaaagtata cgatatcgtt
      961 gtcgctgtga ttctggatcc cgaaccagcc cttatccggc cggcgtccgc gttgaggctc
     1021 gaaatcaaac ttgcgcgtcg gcactggctc gccttcgcga taatcggtga ccttcacacc
     1081 attgacatag acgatggttc gcggtccgtc aagcgtaata tccatcgtat tccattccgg
     1141 ccccggctta ccgggtttgg cgagcggttt tgtcagcgag tacagggtac ccgtgatatg
     1201 atagtcgtcc tcattggatt tttcggggtg gttgtcgatc tgtacctcat agccgtaatg
     1261 taccggcatc cattcctcgc gcggctctat cggaatgcgt atgaatactc ccgaattgtc
     1321 gttttcgtcc cgcatcttaa aaacaaggtg aaatgagcaa ttgctgaaag gtccgcctgc
     1381 ccagtacagc agccccatac caccatgtcc gcgtaacaag ccgttttcga cgctcatgct
     1441 cccgggcccg acgtgtttcc atcccgtgag atcccggccg ttgaaaagct gctgaacgcg
     1501 tcgcggcgga gttggtggcg cgcccgcgaa aagagagggc aggagagttg cgataagtag
     1561 aagtttgtag ttcatacagc aggcttgacg tctaaaggta ctaagatact attatctgcc
     1621 gataataccg tccgccgtgg tatgctcttt gcggccatgc cggtatgaag ccagctccag
     1681 accaatccga ccattccgag gcagccgcag aacaaaaggg gggatccgca agccgtcatt
     1741 ccgggatcgc cgcagaaagc ggggttaccg gcagccgccg ccttttcgtc aagcaaggtc
     1801 ttcgggcggg cctcatcaca cttacgggag gagccgtcct ggctgcatgt tcgggcggaa
     1861 agggggagga gaaagaagta tctccgccgg aagacctgat gcaggaacat ggactgctg
//
LOCUS       DASYSV010000615         8237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2432480, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000615 DASYSV010000000
VERSION     DASYSV010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432480"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VGQ51_19195"
     CDS             <1..524
                     /locus_tag="VGQ51_19195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEV9038775.1"
                     /translation="YGCPAPGRTTVTATTEAGPTITQQGSLLVSSVAANNQWYLNDSL
                     LTDSTTQQIQPFLPGSYYTKIVDQNTGCVLTSNAIVWTPSSGNANQRIGLRVGPNPNT
                     GAFNVSFYMTDGGDLSIGLYDMLGQQVLRQDYGAFTGAFGQTFSTTAASGVYTLKIVH
                     GSNTYYSRVIIKH"
     gene            599..1339
                     /locus_tag="VGQ51_19200"
     CDS             599..1339
                     /locus_tag="VGQ51_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9038776.1"
                     /translation="MLQPWITGKIIRIEQEASQTRRFWIQIPAMESFVFEPGQFVTLD
                     LPIHEKLNKRIRSYSIASWPDGTNVIELVIVLLEGGAGTNYLFNHCDVGSELTLRGPQ
                     GIFTLPDNIEKDLFFICTGTGIAPFRSMSNHILNCNIAHRDIYLIFGCRKFEDALYRA
                     ELTALAEKIPCFHYIPTFSRETPDPMQPQIRTGYVHAIYEEVCRRQESLQPASFFLCG
                     WKNMIDEAKQRIQALGYDRKSIHQELYG"
     gene            complement(1415..1693)
                     /locus_tag="VGQ51_19205"
     CDS             complement(1415..1693)
                     /locus_tag="VGQ51_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038777.1"
                     /translation="MSITFDWIINPIVLMLAGSCGIIMGFVFARVKLVKARARIRKLE
                     TDLLSSNRETLEAQRAFVELESRLKDQAIPVIPMKITGKENSKEKATK"
     gene            complement(1867..3306)
                     /locus_tag="VGQ51_19210"
     CDS             complement(1867..3306)
                     /locus_tag="VGQ51_19210"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEV9038778.1"
                     /translation="MANDSNRFQAIISHCKEYGFIFPSSEIYDGLSAVYDYGQWGSEL
                     KKNIKDHWWKSMTQLQENIVGIDAAIFMHPTVWKASGHVDNFSDPMIDNKDSKKRYRV
                     DHLIESRIEELETQGKKSDADVLQTEMDRLLAASDFAGLKQLIEDLKIKCAVSGTSNW
                     TDVRQFNLMFSTQLGSVSEEASEIYLRPETAQGIFVNFLNVQKTGRMKIPFGIAQTGK
                     AFRNEIVARQFIFRMREFEQMEMQYFIRPGTQKEWYEHWKTARMNWHRGLGIPAEKYR
                     FHDHVKLAHYADAACDIEYEFPIGFKEVEGIHSRTDFDLKNHQEYSRKKMQYFDPEIN
                     QNYIPYVIETSIGLDRTILMVLSEAYTEQDLSTPEKTDSRVVLKFPAWLAPVKLAVLP
                     LVKKDGLPEIARQIMETCKPQFRCFYEEKDTIGKRYRRMDAIGTPFCVTVDQQSKEDG
                     TVTIRYRDTMKQERIPLSEVAAIVNRHLV"
     gene            complement(3373..4599)
                     /locus_tag="VGQ51_19215"
     CDS             complement(3373..4599)
                     /locus_tag="VGQ51_19215"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEV9038779.1"
                     /translation="MQIYQVDAFADRIFQGNPAAVVPLTEWLPDEMLQRIAMENNLSE
                     TAFFVREKERVGAKASDGEAAGSKVSDGGAYSIRWFTPTVEVDLCGHATLASGHVLFE
                     HLGHPGDSILFHTRHAGDLQVLRGDAPGRYTLNFPETPPEPADADSLGIVFEGLGIPA
                     AESVFKGPYDYLVVLESQREVEALRPDFRKLAAAPGRGVIVTAPGDRSVDFVSRGFFP
                     QSGIDEDPVTGSAHSMLVPYWASRLGKRRLSAVQLSARRGALDCRLDRGRVYMTGSAH
                     TFLKGELMIAEGNGIPAPGDRREMLTLHGAQRLVDEWIRSTGVRYFSELTNMAILTEE
                     VGEVARLMSRLYGDQSFKPSDKGRELGDELADVLWVLMCIANQTGVDLTSALGENFAK
                     KNMRDAARHRENEKLQ"
     gene            4772..5452
                     /locus_tag="VGQ51_19220"
     CDS             4772..5452
                     /locus_tag="VGQ51_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9038780.1"
                     /translation="MKILIVEDEIDLAQSMATYLKGENYLCEIANDCRSAMEKIDLFD
                     YDCILLDISLSDGNGLTVLKELKANNKTDGVIILSAKNSIDDKIDGLNLGADDYLSKP
                     FHLPELNARIAAIIRRRRFEGNKTLVLNELTIDLVGKTVVVGQEPVDLTRKEYDLLLY
                     LASNKNRVISKNAIAEHLSGEQAMSYDNLDFIYSHMKNLKRKLHQAGCGDYIRSLYGM
                     GYKFEVRT"
     gene            complement(5506..5958)
                     /gene="dtd"
                     /locus_tag="VGQ51_19225"
     CDS             complement(5506..5958)
                     /gene="dtd"
                     /locus_tag="VGQ51_19225"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEV9038781.1"
                     /translation="MRAVIQRVSRASVTIDGKIKSSISQGLLVLVGIEDADTAADVEW
                     LSGKIVGLRIFNDAEGVMNVSVKDISGDILLVSQFTLHASTKKGNRPSYIRASKPEIA
                     IPLYQLVIDGLGAVLGKKVLTGEFGADMKVELLNDGPVTIVMDTKNKE"
     gene            complement(5934..>8237)
                     /locus_tag="VGQ51_19230"
     CDS             complement(5934..>8237)
                     /locus_tag="VGQ51_19230"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV9038782.1"
                     /translation="RIKPYIGIIGGLAYAYSTYSAVIVAVGHDTKMQSIALIPGVIGA
                     LILICEKKYWLGIVLLTLFTGLMVSFNHIQIIYYTMIIAVGLLLGYGIPWLRHREGRR
                     LVRTVALTLGAVVIGILSNAVTLFTTYDSSRESIRGGSELADAQGNYTKDGLSESAAF
                     DFSMYKLEPLVMLVPDIYGGSTDLQLPADRSKAVRVLDKMPTGVAELVGESGPRYYWG
                     GVGELFSGPPYVGAIIILLALCGFFVLGNRHKWWILAVCALTIVMSWGSYFDSFNHLL
                     LKYLPMYNKFRAPSMILVVPTFLFCMMAVLTLQKIVSEKDRVALWRRYRWGLLAMAGI
                     FVALGVLYFRFNYSSPWDADLLARANGMGRTAVGYITVFLRGLHEDRQELFGRSLMRS
                     LILVTAAALIIAIYLRRRFRPAYMLAIVGVLAFADVLSVDLNYLNTDNYKETAEYQKN
                     FAASNADKEIMADKGYYRVFDLRDSMSNALTYGAMTAYFHNSIGGYHAARLKIYEDLI
                     NRQLFNFPNCAPVINMLNTRYIIKPLTGGRDSVVRNRGSLGPVWFVKGVRYERSAQDV
                     MNALSKFHPADTAILFAKDSSKVKIDGPGGIGGIGGIGGIGSIGGIGGGDHGTDSTGY
                     IRLVKNDNDVISYVSQSRHRQFAVFSEVYYDRGWEAWVDGREVPILRTNYVLRGLSLP
                     PGRHVIRFFFRPLSYYLGRQIQWMASIVFLLMVAGMVIATWQESPFYKARLRRTEGMA
                     VGEEDGGAVSGVQDVPSLEKCEPLSNA"
BASE COUNT         2005 a         2525 c         1867 g         1840 t
ORIGIN      
        1 tctacggatg tccggccccc ggaagaacga ccgtgacggc gaccaccgaa gcgggaccca
       61 ccattacgca gcaaggcagc ctgctggtca gcagcgtcgc ggcaaataac cagtggtacc
      121 tgaacgatag tctgctcacg gattccacca cccaacagat ccagccattc ctgccgggct
      181 cgtactatac gaagatagtg gaccagaata cggggtgcgt gctcacgtct aacgcgattg
      241 tatggacgcc aagctcaggc aacgccaacc agcgcatcgg gctgagggtc ggaccgaacc
      301 cgaacacggg agcattcaat gtgtccttct atatgaccga cggcggcgat ctcagcatcg
      361 gtctctatga tatgctgggt cagcaagtac tcaggcagga ttatggcgcc ttcacgggag
      421 ctttcggcca gacgttcagc accactgcgg catcgggagt ttacacattg aagatcgttc
      481 acgggtcaaa cacttattat tcgagggtaa tcatcaaaca ttaacgcacc aatataaccg
      541 acaagcacaa accatgctgc aacctcggca acatatcatc ttcaatcgct aactaactat
      601 gctgcaaccc tggatcacgg gaaagatcat acgcatcgaa caggaagcat cgcaaaccag
      661 gagattctgg attcagatcc ctgcgatgga atctttcgtc ttcgaacccg gacagttcgt
      721 gacgctcgat ctgcccattc atgagaagct gaacaaacgc atccgtagct attccatcgc
      781 gtcctggccc gacggcacaa atgtcatcga gctggtgatc gtcctgctgg agggaggcgc
      841 cggcacgaac tacctgttca atcattgcga tgtcggcagc gagctgaccc tccgcggacc
      901 gcagggcatc ttcaccctgc ccgacaacat cgaaaaggat ctctttttca tttgtaccgg
      961 caccggcatc gcgccgttcc gctccatgag caatcatatc ctcaactgca acatcgccca
     1021 tcgcgacatc tacctgattt tcggctgtcg taaattcgag gacgcgcttt acagggccga
     1081 attgacggcc ctggcagaga agataccctg ttttcattat atcccgactt tctcgagaga
     1141 gacgcccgat cccatgcagc cgcagatccg cacgggctat gtacatgcta tctatgagga
     1201 ggtgtgccgc cggcaggagt ccctgcagcc agcaagcttt tttctatgcg gatggaagaa
     1261 tatgatcgat gaggccaaac agcgtatcca ggccctcggt tatgaccgaa aatcgattca
     1321 ccaggaatta tacggctaac cagccgcgcg catcccaccg gtcccctttg ccgggccgga
     1381 cattccccgt gtcgcgccag cgaccgggac attattactt cgtcgccttt tctttggaat
     1441 tttctttccc cgttatcttc atcggtatga caggtatcgc ctgatccttc aaacgactct
     1501 ccaactccac aaaagctctt tgagcctcca gggtttcccg attactactc aaaaggtcgg
     1561 tctcaagctt tctgatcctt gcccttgcct tcaccaattt caccctcgca aagacaaaac
     1621 ccatgattat cccacaacta ccggcaagca ttagaacgat agggttgata atccaatcga
     1681 atgtgattga cattgcttaa ttgatttttt aattcagatg gacagaagtt aggtccttca
     1741 tgaggatttg tcgatctttt cagggatagt ggtagagcgg ggctagtaaa tcatcaatat
     1801 ctcactacgg tcaaatatac cgacaaatta atacaaattc ccaacaaaaa gatcgataat
     1861 ttttttttat accagatgcc ggttaacgat agccgcaact tcgctgaggg ggatccgttc
     1921 ctgcttcatg gtatcccgat aacggatggt aacggtacca tcttccttgg actgctgatc
     1981 caccgtcaca cagaacggag tgccgatagc atccatccgg cgataccgtt tgccgatggt
     2041 gtccttttcc tcatagaaac accggaattg cggtttacag gtctccatga tctgccgggc
     2101 tatctccggc aatccatcct ttttcaccag cggcagaacg gccagcttca ccggggctaa
     2161 ccacgccggg aacttcagca cgacccggct atcggttttt tccggcgtac tcagatcctg
     2221 ttccgtatag gcctcactca acaccataag gatcgtacgg tcgagaccta tggaagtttc
     2281 gattacatat ggaatataat tttgattgat ctccggatcg aaatactgca tcttctttct
     2341 gctgtattcc tggtggttct tcagatcgaa atcggttcgc gaatggatgc cctccacctc
     2401 cttgaaacca atcgggaact catattcgat atcacaggcc gcatccgcat aatgcgccag
     2461 cttcacgtga tcatggaacc ggtatttttc ggccggaata cccagccccc gatgccagtt
     2521 catcctggcc gtcttccaat gctcatacca ttctttctgt gtgccgggac gaatgaagta
     2581 ctgcatctcc atctgctcga attcccgcat ccggaaaatg aactgccttg cgacgatctc
     2641 attccggaaa gcttttcccg tctgcgctat accaaacggg attttcatac ggcccgtttt
     2701 ttgcacattc aggaaattca caaaaatacc ctgggccgtc tccggacgca gatagatttc
     2761 gctggcctct tccgagacgc tgcccagttg cgtggagaac atgagattga actgacggac
     2821 atccgtccag ttagacgtgc cactgacagc gcattttatc ttcaggtctt cgatcagctg
     2881 cttcagtccc gcgaaatcac ttgccgccaa cagacgatcc atctctgtct gcaggacatc
     2941 ggcatcgctc tttttcccct gcgtctccag ctcctcgatc cgcgactcga tcaaatggtc
     3001 gaccctgtat cgcttcttgc tgtccttatt atcgatcatc gggtcactga aattatcgac
     3061 atgcccgctg gccttccaca ccgtcggatg cataaatatg gcggcatcga tacccacgat
     3121 attctcctgc aactgcgtca tgctcttcca ccaatgatcc ttgatattct tcttcagctc
     3181 gctaccccat tgaccatagt cgtatacggc gctgagcccg tcgtatatct cgctggaagg
     3241 gaaaataaag ccatattctt tacaatgcga aatgatcgcc tgaaacctgt tgctgtcgtt
     3301 tgccataata tgaacgctaa attctttcct gaatgccgct ttcagcagcc atggcggcaa
     3361 atataaacca atctactgca acttctcatt ttccctgtgc ctcgcggcat cccgcatatt
     3421 ctttttcgca aaattctctc ccaatgccga ggtcagatcg acacccgtct gattcgcaat
     3481 gcacataagc acccacaaca catccgccag ctcatccccc aattccctgc ccttatcgga
     3541 tggcttgaaa gactgatccc cataaagcct gctcatcagc cgcgccacct cgcccacctc
     3601 ttcggtgagt atcgccatat tggtcagctc gctgaaatac cggacgcccg tgctcctgat
     3661 ccattcgtcg accaaccgct gagcgccatg caatgtcagc atctctctgc gatcacccgg
     3721 cgccggtatg ccatttcctt ccgcgatcat caattccccc ttcaaaaatg tatgcgccga
     3781 tcctgtcata tacactcgtc cgcgatcgag ccgacaatcc aacgctcccc gccgtgccga
     3841 caattgaaca gctgaaagcc tcctcttccc caacctgctc gcccaataag gtaccagcat
     3901 actatgcgca gaccccgtga ccgggtcctc atcgatgccc gactgcggaa aaaatccacg
     3961 cgagacgaaa tccacactcc tgtctcccgg cgccgtcacg atcacgccac gaccgggcgc
     4021 agctgccagc ttcctgaagt caggacgcag cgcctccacc tcacgctgac tttccagcac
     4081 cacgagataa tcataaggac ccttgaacac cgattccgca gcaggaatac caagcccctc
     4141 gaaaacgata ccgagcgagt ccgcgtccgc aggctctggc ggggtttccg gaaaattcaa
     4201 agtgtaacga ccgggagcat ctccccgcag cacctgcaga tccccggcat gacgcgtatg
     4261 aaaaaggatc gaatcacccg gatgacccag atgctcgaac aggacatgcc ccgatgccag
     4321 cgtcgcatga ccgcataggt cgacttccac cgtcggcgta aaccatcgga tcgaatacgc
     4381 tcccccatcc gaaactttcg acccggctgc ttcgccatcc gaagctttcg ccccgaccct
     4441 ctccttctca cgaacaaaaa aagcagtctc ggataaatta ttctccatcg caatacgctg
     4501 aagcatctca tctggcagcc attcggtgag cggcaccact gctgcaggat tgccttgaaa
     4561 aatccggtcg gcaaaagcgt ctacctgata gatttgcata aattgtggct tgaaatctcc
     4621 aaagttaccc gctttgagat acagtatctc tacagaatac catctttatt ttaccgctgc
     4681 aaagacccga tacccatcag gtcattgcac ctaactgcca atattcgggt acgagtccgt
     4741 ttgttgttaa aggcctccaa atagttgcac catgaaaatc ctcatcgtcg aagacgagat
     4801 cgatctggcc caaagcatgg ctacctacct gaaaggagaa aactatctgt gtgagattgc
     4861 aaacgattgc cggtcggcga tggaaaagat cgacctgttc gattacgatt gtatcctgct
     4921 ggatatctcg ctcagcgacg gcaatggact caccgtcctg aaagaattga aagccaataa
     4981 caagaccgac ggtgtcatca tcctctccgc caagaattcc atcgacgaca agatcgacgg
     5041 gctcaatctg ggtgcagacg attatctcag taaaccgttt catttgcccg aactgaatgc
     5101 ccgcattgcc gccatcatcc gccgccgccg attcgaagga aataaaacac tggtattgaa
     5161 tgagttgacg atcgatcttg tcgggaagac cgtagtggtc gggcaggagc cggtcgacct
     5221 cacccgcaag gaatacgatc tgctgctcta tctcgcctcc aataagaacc gcgtcatctc
     5281 caagaacgcc atcgccgaac atctctccgg cgaacaggcg atgagctatg ataatctgga
     5341 tttcatttac tcacatatga agaacctcaa acgcaagctg caccaggctg gttgtggcga
     5401 ttatatccgc tcgctgtacg gcatgggcta caaattcgag gtccgcacct gacgccgcac
     5461 catctttgca cctaacgccc gcacacctca cgacccgcac ccccttcact ccttattctt
     5521 tgtatccatc acgatcgtca ccggcccgtc attcaacaat tccaccttca tatccgcgcc
     5581 gaactcccct gtcaatacct tcttccccag caccgcccca agtccgtcga tgaccagctg
     5641 atacaacggt atcgctattt caggcttact ggcccgtata tacgaaggcc tattgccctt
     5701 cttcgtggaa gcatgcaggg taaactgact taccagcaga atatccccgc taatatcctt
     5761 cacggaaaca ttcatgacgc cctctgcatc attgaagatg cgcagaccca cgatcttacc
     5821 gctcagccat tcgacatcgg ctgccgtatc ggcatcctcg atgccgacca gtaccagtaa
     5881 cccctgacta attgaggatt ttatcttccc atcgatagta acggaagcac ggcttacgcg
     5941 ttggataacg gctcgcattt ttcgagcgaa ggtacatcct gaaccccgga taccgctccg
     6001 ccatcttctt caccaacagc cattccctcc gtccgtctca gccgcgcctt ataaaaagga
     6061 gattcctgcc aggtggcgat caccatgccc gctaccatca gcagaaaaac tatcgaagcc
     6121 atccattgta tctgccgccc caggtaataa gacaacggcc ggaagaaaaa ccggatcaca
     6181 tgcctcccgg gcggcagcga aagaccccgc aatacatagt tcgtccgcag gattggcacc
     6241 tctctcccgt ccacccaggc ttcccaaccc ctgtcataat atacctcgct gaacaccgca
     6301 aactgtctgt gccgtgattg cgatacatac gagatcacat cattatcgtt cttcaccagg
     6361 cggatatagc cggtcgaatc ggtcccatga tcgccaccgc caatgccgcc aatactacca
     6421 attccgccaa tgccaccaat accaccaatt ccgcccggcc catcgatctt caccttgctg
     6481 ctgtcctttg cgaacaggat cgccgtatcc gctggatgga atttgctcag cgcattcatg
     6541 acatcctggg ccgatctttc atagcggacc cccttaacga accacaccgg acccagcgac
     6601 ccccggttac ggaccaccga atcacgacca cccgtcaggg gcttgatgat ataccgcgta
     6661 ttgagcatat tgatcacggg agcgcagtta gggaaattga acagctggcg attgatcagg
     6721 tcttcataga tcttcagcct ggccgcatga tatcccccga tggaattgtg aaaataagcc
     6781 gtcatggccc cataggtcaa cgcattcgac atactgtcgc gaagatcgaa gacccggtaa
     6841 tagcccttat cagccatgat ctccttatcg gcgttggagg cggcgaagtt cttctggtat
     6901 tcggcggttt ctttgtagtt gtcggtgttg agatagttga ggtccaccga gagcacatcc
     6961 gcgaaggcca gcaccccgac gatggccagc atataagccg gccgaaaccg tcgccgcaga
     7021 taaatcgcga tgatcaacgc tgccgcggta acaaggatga ggctccgcat caggctgcga
     7081 ccgaacagct cctgtctgtc ctcatgcaag cctcgcagga agacggttat ataacccacc
     7141 gccgtccggc ccatgccgtt ggccctcgca agcagatcgg cgtcccatgg actggaatag
     7201 ttgaaccgga aatagagtac ccccaatgcc acgaagatcc ccgccatcgc gagcagcccc
     7261 cagcgatacc ggcgccatag cgcgaccctg tccttctccg agacgatctt ttgcagcgta
     7321 agcaccgcca tcatacagaa caggaaggtg gggacaacca ggatcatgct gggagccctg
     7381 aacttattgt acatgggcag atacttcagc aacagatggt tgaaggagtc gaaataagag
     7441 ccccagctca tcacgatggt gagcgcgcag acggccagta tccaccattt gtgcctgttg
     7501 cccagcacga aaaatccgca cagcgcgagc agaatgatga tagccccgac atagggcgga
     7561 ccggaaaaga gctctccgac tccaccccag taatagcgcg gaccgctctc acccaccagc
     7621 tcggctaccc cggtgggcat tttatccaaa acacgcacgg ctttcgacct gtccgccggc
     7681 agctgcagat cagtactgcc accatagatg tccggcacca gcatcaccaa cggctccagc
     7741 ttgtacatgc tgaaatcgaa agcggcggat tccgacagac catccttggt atagttgccc
     7801 tgcgcatcgg ccagctcgct gccgcccctg atgctctcgc gcgaggaatc ataggtggtg
     7861 aaaagcgtca ccgcattgct caggataccg ataaccacgg cgcccagcgt cagggcaacg
     7921 gtccgcacaa gacggcggcc ttcacggtgc cgtagccagg gtatcccata tcccagcagg
     7981 aggcccacgg cgatgatcat tgtataatag atgatctgta tgtggttgaa ggaaaccatc
     8041 agccccgtga atagcgtgag caggacgatc cccaaccagt atttcttttc gcagatcagg
     8101 atcagcgcgc cgatgactcc cgggatgagt gcgatggact gcatcttggt atcatgtccc
     8161 accgcaacga tgactgcact ataggtggaa taggcatacg ccagccctcc gatgatgccg
     8221 atatatggct tgattcg
//
LOCUS       DASYSV010000616         3501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1408043, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000616 DASYSV010000000
VERSION     DASYSV010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408043"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VGQ51_19235"
     CDS             <1..199
                     /locus_tag="VGQ51_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEV9038783.1"
                     /translation="ISDKLGIPVSTTHTIAGSIMGVGATKRLSAVRWGVTINLLWAWV
                     LTIPVSALMAAVVYFIVNLFR"
     gene            273..1136
                     /gene="rfbA"
                     /locus_tag="VGQ51_19240"
     CDS             273..1136
                     /gene="rfbA"
                     /locus_tag="VGQ51_19240"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV9038784.1"
                     /translation="MKGIILAGGSGTRLHPITYAISKQIMPVYDKPMIYYPLSTLMLA
                     GIREILIISTPHDLPLFRKLFGDGSQLGLSFSYAEQPLPNGLAQAFVIGEDFIGKDPV
                     ALVLGDNIFYGAGLGKQLEKNTRPDGGIVYAYQVSDPERYGVVEFDADNRAISIEEKP
                     VKPKSNYAVPGLYFYDNSVVGISKNLKPSARGEYEITDVNKEYLRQGKLTVSILDRGT
                     AWLDTGTFDSLMQAGQFVQVIEQRQGIKIACIEEIAWRKGFINREQVEKIAQPLLKSG
                     YGQYLVNLIHS"
     gene            1153..2211
                     /gene="galE"
                     /locus_tag="VGQ51_19245"
     CDS             1153..2211
                     /gene="galE"
                     /locus_tag="VGQ51_19245"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV9038785.1"
                     /translation="MKKILVTGGCGYIGSHTIVDLIASGFEVICVDSNVRSSPRVLEG
                     IEKITGKKVKNYKVDLCNYDDTFAIFQENENIAGIIHFAAYKAVGESVEHPLMYFENN
                     IMSLINLLKCVQEFEIPHFVFSSSCTVYGNPDEIPVTEQTPPKPAESPYGYTKQMGEQ
                     IINEFSKATTKTKSILLRYFNPVGAHPSILIGELPVGKPANLVPVITQTAIGRLPQMT
                     VYGDDYPTRDGSCIRDFIHVCDIAHAHTLALQYMIDKKVVKNAEVFNLGTGNGVTVLE
                     AIRAFEKVSGVKLNYMIGPRRPGDVVAIYANNDLARKQLGWNIEYGLEDMMSTAWKWE
                     QKLVADEKFYNGKFSKFN"
     gene            2276..3235
                     /locus_tag="VGQ51_19250"
     CDS             2276..3235
                     /locus_tag="VGQ51_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV9038786.1"
                     /translation="MLKDIQVTGQKDTRSGFGDGIAEVARKNPNIVALTADLAGSMKL
                     NTFMKEFPERYVQCGIAEANMIGIAAGLTIGGHIPYTTTFANFSTGRVYDQIRQSVAY
                     SGKNVKICASHAGLTLGEDGATHQILEDIGMMKMLPGMTVIVPCDYAQTKAATIAIAD
                     HVGPVYLRFGRPVWPIFTAGLPFEIGKAQVLSEGSDVSILACGHLVWKAIEAGKILEE
                     KGISVEVINLHTVKPLDEAAILRSVQKTGCAVTAEEHNIIGGLGSAVAQTVGRNFPIP
                     IEFVGTNDTFGESGKPEELLKKYHLDTPDIVAAAEKVIARKKK"
     gene            3253..>3501
                     /locus_tag="VGQ51_19255"
     CDS             3253..>3501
                     /locus_tag="VGQ51_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV9038787.1"
                     /translation="MTIQDTELLLQFRDPDSREKAFTAIIKKYQEKLYWHIRRMVVEH
                     EDANDVLQNVFIRVWNGLDNFREDSQLYTWLYRIATNEC"
BASE COUNT          911 a          838 c          970 g          782 t
ORIGIN      
        1 tatttccgat aaattgggga tcccggtaag cactacccac acgatcgcgg gcagcattat
       61 gggcgtggga gcgaccaaac ggctttcggc agtgcgatgg ggagtgacga tcaacctgct
      121 ttgggcctgg gtgttgacca tccctgtaag cgccctgatg gcggcggtgg tttattttat
      181 cgttaacctg ttcaggtagg atgggttcag gtgattgccg gatcgcataa tttttctaac
      241 tttcgaagat tttcaaaaat gtacagacag ctatgaaggg aattattctg gcaggcggat
      301 cgggaacgag gctacatcca ataacctatg ccatcagcaa acagatcatg ccggtgtatg
      361 acaaaccgat gatctattat cccctgtcaa cattgatgct ggcggggata agggagatcc
      421 tgatcatttc cacgccccat gatcttccgt tgttcagaaa actgttcggg gacgggtcgc
      481 agctggggct ttctttttcc tatgccgagc agccgctgcc caacgggctg gctcaggcct
      541 tcgtcatcgg ggaagacttt atcggcaagg atcccgtggc gctggtgttg ggagacaata
      601 tcttttatgg cgcgggtctg ggcaaacagc tggagaagaa tacgagacct gacgggggca
      661 tcgtttatgc ctaccaggtc agcgatcccg aacgatatgg tgtggtggaa ttcgatgccg
      721 acaaccgggc tatctccatc gaggaaaagc cggtcaaacc aaaaagcaat tatgccgtac
      781 cgggcctcta tttctatgac aattcagtgg tggggatctc gaagaacctg aaaccttcgg
      841 cgcgcgggga atatgagatc accgatgtca acaaagaata tttacggcag ggtaaactga
      901 ccgtcagcat attggaccgg gggacggcct ggctggacac cgggacattc gactcgctga
      961 tgcaggccgg ccagttcgtc caggtgatag agcagcggca gggcatcaag atagcgtgta
     1021 tcgaggagat tgcgtggaga aaaggcttca tcaacagaga gcaggtggag aaaatagccc
     1081 agcccctgct caagagcggg tatgggcagt atcttgtcaa tcttattcat tcgtaaattc
     1141 gactaaccaa tcatgaagaa aatccttgta accggcggat gcggatatat aggctcgcat
     1201 acgatagtcg atctgatcgc ctcgggtttc gaagtgatct gtgtcgacag taacgtacgt
     1261 tcgtccccgc gggtgctcga agggatcgaa aagattaccg ggaaaaaggt caagaactat
     1321 aaagtggacc tatgcaatta tgacgacact ttcgcgatct tccaggagaa cgagaatata
     1381 gcggggatca ttcattttgc cgcttataaa gcggtgggtg agtcggtaga gcatcccctg
     1441 atgtatttcg agaacaacat catgtcgctg atcaacctgc tcaaatgcgt tcaggaattc
     1501 gagataccgc atttcgtatt ttcctcttcg tgtacggtgt atggcaaccc cgacgagata
     1561 ccggtgaccg aacagacgcc gccgaagccg gccgaatcgc cgtacggcta tacgaagcag
     1621 atgggcgagc agatcatcaa tgaattctcc aaggccacga cgaagaccaa atccatcctg
     1681 ctccgttatt tcaacccggt gggcgcgcat ccgagtatcc tcatcggcga attgccggtc
     1741 ggaaagcccg ccaacctggt tccggtgatc acccagacgg ccatcggccg cttgccccag
     1801 atgacggtat atggcgatga ctatccgaca cgcgacggct cctgtatccg ggatttcatc
     1861 catgtctgcg atatcgccca tgcgcatacg ctggccctgc aatatatgat cgacaagaag
     1921 gtggtcaaga acgccgaggt attcaacctg gggacgggta acggcgtaac ggtgctcgaa
     1981 gccatccggg ctttcgagaa ggtgagcggc gtaaagctga attatatgat cggaccgcgc
     2041 cgcccgggtg acgtagtagc gatctatgca aataacgatc tggccagaaa acaactggga
     2101 tggaacatag agtacgggct cgaggatatg atgtccaccg cctggaaatg ggaacagaag
     2161 ctggtggccg atgaaaagtt ctataacggg aagttcagca agttcaacta gacaatcttt
     2221 ttgcgccatc ttttgacgca atacctttac tttgtaaggc taaaacacaa cattaatgtt
     2281 aaaagacatt caggtaaccg gacagaaaga cactcgaagc ggatttggag acggcattgc
     2341 agaagtagca cgtaagaacc ccaatatagt tgcacttacg gccgacctgg ccggatcgat
     2401 gaagctgaat acctttatga aggaatttcc ggaacgctat gtccagtgcg gcatcgccga
     2461 agcgaacatg atcggtatcg ccgcgggttt gaccatcggc ggccatattc cttataccac
     2521 gaccttcgcc aatttttcta ccggccgggt ctatgatcag atcagacagt cggtggctta
     2581 ttcgggaaaa aatgtcaaga tctgcgcatc ccatgccggg ttgacccttg gcgaggacgg
     2641 agcgacacac cagatattgg aagacatcgg catgatgaaa atgttgcccg ggatgaccgt
     2701 gatcgttccc tgcgattatg ctcagacaaa ggcggccacg atcgccatcg cggatcatgt
     2761 cggtcccgtc tatcttcgat tcggccgccc ggtatggccg atctttactg caggacttcc
     2821 attcgaaata ggtaaggccc aggtactctc cgaaggtagc gacgtatcga tcctcgcctg
     2881 cggtcatctc gtctggaagg caatagaggc cggaaagatc cttgaagaaa aaggaatatc
     2941 ggtcgaagtg atcaatcttc atacagtgaa gccgttggat gaggcggcca tcctgcgatc
     3001 ggtacagaag acggggtgcg cggtgacggc tgaagagcat aatattatcg gtggactggg
     3061 ttccgccgtg gcgcagaccg tgggacggaa tttcccgatc ccgatcgagt tcgtcggcac
     3121 taatgatacc ttcggtgaaa gcggcaaacc ggaagagctg ttgaagaaat atcatctcga
     3181 tacgcccgat atcgtcgcag cggctgagaa agtgatagca agaaagaaaa aataaataac
     3241 tagatttaaa ctgtgaccat ccaagacacc gagctgctct tgcaattccg tgatcctgat
     3301 agccgggaga aggcgttcac cgccattatc aaaaaatatc aggaaaagtt gtattggcat
     3361 atccgaagga tggtggtcga gcacgaggat gcgaatgacg tattacaaaa tgtattcata
     3421 cgcgtctgga atggtcttga taatttccgg gaggattccc agctgtatac ctggctgtac
     3481 cggattgcca ctaatgaatg t
//
LOCUS       DASYSV010000617         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_2432681, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000617 DASYSV010000000
VERSION     DASYSV010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432681"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VGQ51_19260"
     CDS             complement(<1..821)
                     /locus_tag="VGQ51_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEV9038788.1"
                     /translation="MEYKFFEAVEKSFDKAAGFTSWDPGILEQIKQCNAVYRMFFPVK
                     IGDKIEVIKAYRVQHSHHKTPCKGGIRFAPSVNLDEVMALAALMTFKCAIVNVPFGGA
                     KGGISIDPKKYTPYELEKITRRYTSELIKKNFIGPGTDVPAPDYGTGEREMAWILDTY
                     MSMRPGEIDALGCVTGKPVSQGGVRGRREATGLGVFYGIREVCHMDEMMTKLKMRPGV
                     EGKTVVVQGLGNVGYHAAKFFREGGAKVIALAEFEGAIMNAAGLNEEEVFQHRKKT"
     gene            complement(845..1078)
                     /locus_tag="VGQ51_19265"
     CDS             complement(845..1078)
                     /locus_tag="VGQ51_19265"
                     /inference="COORDINATES: protein motif:HMM:NF040113.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038789.1"
                     /translation="MYKKVYAFGLLVIGWMWSLKAMAQDGAGDRKVEMADSFRANGKI
                     YVVIAVALIILLGLLFYVSRLDRKISKYEKEVK"
     gene            complement(1071..1745)
                     /locus_tag="VGQ51_19270"
     CDS             complement(1071..1745)
                     /locus_tag="VGQ51_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEV9038790.1"
                     /translation="MLRHSWWKILCALLLFYTIVAGFLGPVPALPILDETIRNLYFHV
                     AMWMAMMITFIVSVIYSIKYLRSNNALYDIYALEYARTGIVFGVLGLVTGSVWARWTW
                     GAFWSNDPKQLGAVIALLIYLAYFVLRNSMTDMDKRARIGAVYNIFAFAMLFPTIWII
                     PRMVESLHPGGMGNPAFDTNDIDSRMRAVFWPGAVPGWTLLGVWITTLRIRLSILEEK
                     RTHSNV"
     gene            complement(1801..2430)
                     /locus_tag="VGQ51_19275"
     CDS             complement(1801..2430)
                     /locus_tag="VGQ51_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEV9038791.1"
                     /translation="MFKKDVLLEVRQQYTFYGVLLYVASTIFVLYLSMGQPEGAVWKG
                     LFWMMQLFICVNAVAKSFLQEGKERLLYFYSIAGARDFILSKLLFNALLMVLMSLVSV
                     GLFLLMLGDPTSDVLWFVLIGSLGGISLSLVFTFLAAIAARAQQSSALMAILGFPLVI
                     PQLLLLNKVSSIAFSEVQGGLAGMIGLLAALDAMIVALTVILFPFLWKD"
     gene            complement(2479..>2814)
                     /locus_tag="VGQ51_19280"
     CDS             complement(2479..>2814)
                     /locus_tag="VGQ51_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038792.1"
                     /translation="DEIKGNLQVRYQYPMNKIRPFIGAGISNTLTINNKSTQTLIDVS
                     NSQNFRQPLFGSADYIRMYRPGAFISLGAEYRKFVLEARAEQTQGLTNMPGVSAPVMN
                     FYLLLGYMF"
BASE COUNT          714 a          817 c          647 g          636 t
ORIGIN      
        1 gtctttttac ggtgctggaa gacctcctct tcattcaaac cggccgcatt catgatggcg
       61 ccttcgaact ccgccagggc aatcaccttc gcaccgcctt cgcggaagaa cttggccgca
      121 tgatagccta cgttgccgag accctgaacc acgaccgtct tgccttccac gccgggacgc
      181 atcttcagct tcgtcatcat ttcgtccata tgacatactt cacggatacc atagaatacc
      241 ccgagacccg tagcttcacg gcgtccgcgg actccgccct ggctcactgg tttgccggtg
      301 acgcagccga gcgcatcgat ctcgccgggc cgcatgctca tataggtgtc gagtatccat
      361 gccatctcgc gctcacccgt gccatagtcg ggagcgggca cgtccgtacc cggaccgata
      421 aaattctttt tgatcagctc ggaagtatac cggcgggtga tcttttccag ctcataaggt
      481 gtatacttct tcggatcgat gctgataccg cccttggcgc cgccgaaggg cacgttgacg
      541 atcgcgcatt tgaaggtcat cagcgcggcc agcgccatca cctcgtcgag gttgaccgac
      601 ggggcgaaac ggataccgcc cttgcagggg gtcttgtgat gcgaatgctg cacgcgatag
      661 gccttgatta cctctatctt gtcgcctatc ttaacgggga agaacatgcg atagaccgcg
      721 ttgcactgtt ttatctgctc caggatgccc ggatcccagc tggtaaagcc agcggcctta
      781 tcaaagcttt tttctacggc ttcgaaaaat ttgtattcca ttttggcaag tttgtgtgtg
      841 tctcttattt aacttctttt tcatacttcg atattttccg gtccaggcgg ctgacataaa
      901 acagcaatcc cagcagaatg atcagggcga cggcgatgac gacatagatc tttccatttg
      961 ccctgaaact gtcggccatt tctactttcc tgtcacccgc gccgtcctgc gccatcgcct
     1021 tcagcgacca catccaaccg ataaccagga gcccgaaggc gtaaactttt ttatacatta
     1081 ctgtgtgttc ttttttcttc caaaatacta agccgtatgc gcagcgtcgt gatccagacc
     1141 cctaacaaag tccagcccgg tactgccccc ggccagaaaa ccgcgcgcat ccgggagtcg
     1201 atatcgttcg tatcgaaggc cggattaccc attcccccgg gatgcagact ctccaccatc
     1261 cgcggaatta tccagatggt agggaataac atcgcaaacg caaaaatatt gtatacggcc
     1321 cctatccgag cccgtttgtc catatccgtc atcgaattac gcaatacgaa ataggccagg
     1381 tagatcagca aggcgatcac agctcccagc tgtttggggt cattgctcca aaaagctccc
     1441 cacgtccatc ttgcccagac agagcctgtc accaggccca gcacgccgaa aacgatgccc
     1501 gtccgcgcat attcaagcgc ataaatatca tacaaggcat tgttcgaccg caggtatttg
     1561 atcgaataga tcaccgaaac gataaaggtg atcatcatgg ccatccacat cgctacatga
     1621 aagtataggt tgcggattgt ctcatctaaa atgggcagcg caggcacggg ccccaggaac
     1681 cccgcaacga tggtatagaa caataagagt gcgcatagta ttttccacca ggaatgacga
     1741 agcataagta cttttataga aggaggtcgg cctcccgttt ggagtgcaaa tttaggtaac
     1801 tcaatctttc cacaaaaacg gaaaaagaat gaccgtaagc gccacgatca tcgcatccag
     1861 agccgccagg agcccgatca tcccggccaa acctccctga acttccgaaa aagcaatcga
     1921 cgagacctta ttcaacagca acagctgcgg gatcaccaat gggaaaccca gtattgccat
     1981 caatgccgag ctttgctgcg cacgcgccgc aatggcggct aagaatgtaa ataccaggct
     2041 cagactgata cctcccagcg aaccgatcaa cacgaaccac aacacatcgg atgtcggatc
     2101 gcccagcatc agcaggaata atccgacact caccaggctc atcaggacca tgagcagcgc
     2161 attgaacaat agtttcgaca ggataaagtc ccttgccccc gcgatggaat aaaaatagag
     2221 cagcctctcc ttgccttcct gcaaaaagct cttcgcaacc gcgttgacgc agatgaacag
     2281 ctgcatcatc cagaacaaac ccttccatac tgccccttcc ggctgaccca tgctcaggta
     2341 taaaacgaaa atggttgagg caacatagag cagcactcca taaaaagtat attgctgcct
     2401 cacctccagc agcacgtctt ttttaaacaa agtcaggata tgtgaaaggg aacgtatacc
     2461 gcaaaatttg cggcaaagct aaaacatata gccgaggagc agatagaaat tcattaccgg
     2521 cgcgctgact cccggcatat tcgtcagccc ctgggtctgt tcggcccgcg cctccaatac
     2581 gaacttccgg tactccgcgc ccagggaaat gaaagcgccc ggtctataca tccggatata
     2641 atctgccgat ccgaacagcg gttgcctgaa attctgggaa ttgctgacat cgatgagcgt
     2701 ttgcgtgctc ttattattga ttgtcaatgt gttagatatg cctgcgccga taaatggccg
     2761 gatcttattc atcggatact gatatctcac ctggagattc cccttgatct cgtc
//
LOCUS       DASYSV010000618         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1278362, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000618 DASYSV010000000
VERSION     DASYSV010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278362"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..964)
                     /locus_tag="VGQ51_19285"
     CDS             complement(29..964)
                     /locus_tag="VGQ51_19285"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV9038793.1"
                     /translation="MAIVWKGVFPALTTKFTAKDELDLKLFEKNLNFQIASGIDGIIL
                     GGSLGEASVLTIQEKERLVKFAVEKAAGELPVILNIAEGSTREALQQASDARAWGAQG
                     LMLLPPMRYSSDHRETVTWFRTIADSTDLPIMIYNNPVDYKTNVTLDMFEELIECSNI
                     QAVKESTRDISNVTRMINRFGGRLKILCGVDTLAMEEMLMGADGWVAGLVCAFPAETV
                     AIYRLVKAGRVEEAMAIYRWFLPVLELDIHTKLVQYIKLAEVQTGIGSEYVRAPRLPL
                     VGTERERILKVIGQSVATRPALPDYLSIHPGKTFA"
     gene            complement(1137..3263)
                     /locus_tag="VGQ51_19290"
     CDS             complement(1137..3263)
                     /locus_tag="VGQ51_19290"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEV9038794.1"
                     /translation="MKRLFLQVVLLAALLPAEAQPEAAPMASYEPTRSEIMDRYHRAH
                     FLDSIATRSIFKAVVRPHWSADHNSFWYRNILKDSVKEYIDVDMVHGTRRKLDAEPAD
                     TLYRSAAFTRYTGRRWASFSTDSVSPDKKWVAFVRDGNVFVREASANRGSAFPFTTDG
                     DTAKPYGQLAWSPDSKYLVGYHIDPVKDSAVYYVLSSVAGTTRGQLRRRPYKQPGDPF
                     TTYEMFIFRLDGRQQVRVNTPKVDFFGAPELHWRYHDSRYFTFERVERGHQRFRIFEV
                     DAQTAQTRTIIDETTRTFIYESRLFTYYLPETNELLMTSEKDGWQHLYLFDLLTGRQK
                     QEVTRGPWVVRNIDSVDAKKREVWFSASGMDAGEDPYYVHYYRIGFDGQHLIGLTPVR
                     ANHIVTFSPDRKYYLDAYSEINVPTVVRLCRTADAREIMEVERADASMYLAAGFRFPE
                     PFHAKGRDGVTEIWGVIVRPTDFDSTKRYPVIENIYAGPQDAFVPKDFTGRYGEMQSI
                     AQMGFIVVQIDGMGTANRSKAFHDVCWKNLADAGFPDRILWIKAMAAKYPYADTSRVG
                     LYGTSAGGQNSLGGLLFHGDFYKAAVSSCGCHDNRIDKQWWNEQWMGYPVGKHYDEQS
                     NVTNAWRLHGDLLLMVGEADNNVPPESTYRVADALIKAHKVFEFLPLPGSDHTDGGPY
                     GRVSRRDFFVRHLLGVEPPHRNEGEL"
     gene            complement(3288..>4383)
                     /locus_tag="VGQ51_19295"
     CDS             complement(3288..>4383)
                     /locus_tag="VGQ51_19295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV9038795.1"
                     /translation="LATPGIVRPGLKWMLNSQSPFYVQPRLSRSLVDWGMKFMKSATP
                     GHVQRSAIPLKDIAVLSQYEYENTWLPLPHFDFAYQHKGLLEIFQTPAGAEHARHTVE
                     KGHELGLDVDLLDAVALQEKEPHTKVNGLGAIFFKCDAHLFPDKLMRGLIRILGEAGV
                     QLIPHQEVTGFEKGNGAVKRLITATGHHYDADAIVVATGSWSREMAALLDTNLPLVPG
                     RGYSVTLADSPYRVNYPAILVEGRAAITPMDGNKIRFGGTMEITSTNTPPRYQRVQGI
                     LNAVKRFFPEFDVPMPPKEKIWYGYRPCSADGLPYIGRIRRYNNVVVATGHSMLGLSL
                     GAGTGRLVSELVDERPTSIDLTPFAVERFG"
BASE COUNT          943 a         1281 c         1174 g          985 t
ORIGIN      
        1 ttgttttttc ggtcatgcga ctcggatatc aggcaaaggt cttcccggga tggatagata
       61 gataatcagg caacgcaggc cttgtcgcga cactctgacc aatgaccttt aatatcctct
      121 cccgttcggt tccgacgagc ggcagtcgcg gggcacggac atattcgctg ccaatgccgg
      181 tctgtacttc cgccagcttg atgtattgga ccagtttggt gtggatgtcg agctcgagca
      241 cggggaggaa ccacctgtag atggccattg cctcttcaac acgtcccgct ttgacgagac
      301 gataaatggc cacggtttcg gccgggaaag cgcatacgag gccggctacc cagccatcgg
      361 cgcccatcag catctcttcc atcgccagcg tatcgacacc gcagaggatc ttgagccgcc
      421 ctccgaagcg attgatcatc cgggttacat tggagatatc ccgtgtcgat tctttcacgg
      481 cctggatgtt ggagcattcg atcagctctt cgaacatgtc gagcgtcaca ttggtcttat
      541 agtcgacggg attgttgtag atcatgatcg gcaggtcggt ggaatccgcg atggtcctga
      601 accaggtgac agtctcgcga tgatccgaac tatagcgcat cggcgggagg agcatcaggc
      661 cctgagcgcc ccaggcgcgt gcgtcggatg cctgctgcag ggcttcccgt gtcgatccct
      721 cggcaatatt cagtatgacc ggcagctcac ccgccgcttt ttcgacggca aacttcacca
      781 gcctttcctt ctcctggatg gtaagcacac tggcttcgcc cagacttccg ccgaggatga
      841 tcccatcgat gcccgaggcg atctgaaaat tcaggttctt ttcaaacagc ttgagatcca
      901 gttcgtcctt agccgtgaat ttagtggtta gcgcggggaa gactcctttc cagacaatag
      961 ccatagaatc gttttttcaa atgtattcag atacgcgtgt cggcgtatat tcatttttaa
     1021 tcaatcatgc gcagaattta accggtctgc cgacggggcg cgaacccgtc tgcgaccggg
     1081 cgcgaagcgg tctttcgacg gggctcgaac cggtctgcgg cgggtgggct gcggtcttat
     1141 aactccccct cattgcgatg gggcggctcg acccccagca gatgccttac aaaaaagtct
     1201 cttcgcgaga ccctcccata ggggccgccg tcggtgtgat ccgatccggg taacggcagg
     1261 aactcgaaga ccttgtgcgc cttgatcagc gcatccgcca cccggtaagt ggactcgggc
     1321 ggaacgttgt tatcggcttc ccccaccatc agcagcagat cgccgtggag cctccaggca
     1381 ttggtgacgt tcgattgctc gtcataatgt ttcccaacgg gatagcccat ccactgctca
     1441 ttccaccact gtttgtcgat acggttgtca tgacagccgc aggacgatac ggccgccttg
     1501 taaaagtccc catggaaaag caggcctccg agggaattct gtccccccgc cgatgtgcca
     1561 taaagtccta cccgcgacgt gtcggcataa ggatattttg ccgccatggc ctttatccac
     1621 aatatcctgt cggggaaccc ggcatcggca agattcttcc aacaaacgtc atggaaggcc
     1681 ttcgaccggt tggccgtgcc cattccgtcg atctgtacca cgatgaaacc catctgggcg
     1741 atgctttgca tctcgccata acgcccggta aaatccttag gtacaaaggc atcctgggga
     1801 ccggcgtaaa tgttttctat taccggatat cgtttcgtcg aatcgaaatc cgtcggccgt
     1861 acgatcacac cccagatttc cgtaacccca tccctaccct tcgcatgaaa aggctccggg
     1921 aaacgaaaac ccgccgccag atacatcgag gcatccgctc gctccacctc catgatctcc
     1981 ctggcatctg cggtgcggca aagccggacc acggtcggaa cattgatctc cgaataggca
     2041 tcgaggtaat atttccggtc gggcgagaat gtgacgatat gattggcccg gaccggggtc
     2101 agaccgatca ggtgctgccc gtcgaacccg atgcgataat aatgaacgta atacggatct
     2161 tcaccagcat ccataccact ggccgagaac caaacctccc ttttcttagc gtcgacgctg
     2221 tcgatattcc ggacgaccca ggggccgcgt gtgacctcct gtttctgtcg gccggtcagc
     2281 agatcgaaaa gatacagatg ctgccatccg tccttctctg acgtcatcaa taattcgttc
     2341 gtttccggaa gataatacgt gaaaagacgc gattcataga taaaggtcct ggtggtctcg
     2401 tcgatgatcg tccgcgtctg cgccgtctgc gcatccactt cgaatatccg gaaccgctgg
     2461 tgacccctct ctaccctttc gaaagtaaaa tagcggctgt cgtgataacg ccagtgcaat
     2521 tcgggagcgc cgaaaaaatc caccttcgga gtgttgaccc tgacctgctg tctcccatcg
     2581 agacggaaga tgaacatctc atatgtagtg aacggatcgc ccggttgttt gtaaggtctt
     2641 cggcgcagtt gcccgcgtgt tgtgccggcc accgacgaca gcacgtaata gacggcggaa
     2701 tcctttacgg gatctatatg ataaccgacc aggtacttac tgtccggtga ccaggccagc
     2761 tgtccatagg gttttgcggt atcgccgtcg gtggtaaaag ggaatgccga tccgcgattt
     2821 gcacttgcct cgcggacaaa gacattgcca tcgcggacaa aggctaccca ttttttgtcc
     2881 ggagaaaccg agtcggtgga aaaggaagcc cagcggcgac cggtatacct ggtgaatgct
     2941 gcgctgcgat acaatgtatc ggcgggctcg gcgtccagct tcctgcgtgt accgtggacc
     3001 atgtccacat cgatatattc cttcacactg tctttcagga tattcctgta ccagaaggaa
     3061 ttgtggtcgg cggaccagtg cggccggacg acggctttga agatgctgcg ggtggcgatg
     3121 ctatccagga aatgggcgcg gtgataccgg tccatgatct ccgaccgtgt cggttcgtac
     3181 gatgccatcg gcgcagcctc cggctgcgcc tccgcgggta gcagggctgc caaaagcacc
     3241 acctgcagaa acaatcgttt catatatgaa gtttttttac gcgcgtatca tccgaagcgc
     3301 tccaccgcaa aaggcgtcaa atcgatgctg gtcggccgct cgtccaccaa ttcactcacc
     3361 agacgccccg ttcccgctcc aagactaagc ccaagcatcg agtgccctgt cgccaccact
     3421 acattgttgt atcgtcgtat cctgccgata tatggcaacc catccgccga acagggccgg
     3481 tagccatacc atattttttc cttcggaggc atcggcacgt cgaattcagg gaagaaccgt
     3541 ttgacggcgt tgagaatgcc ctgcacccgc tgataacgcg gtggggtatt ggtcgaagtg
     3601 atctccatcg ttccgccgaa ccggatcttg ttgccatcca taggcgtgat cgcggcgcga
     3661 ccttccacga ggatagcagg atagttgaca cggtatggag agtctgcgag ggttacggaa
     3721 tatcctctcc ccggaaccag tggcaaattt gtgtccagca gggcagccat ttcacggctc
     3781 caggagcctg tcgccacgac gatcgcatcg gcatcgtaat gatgtccggt ggcagtgatc
     3841 aggcgtttga cggcgccgtt gcctttctcg aaacccgtaa cttcctggtg cgggatcagc
     3901 tgaactcccg cctcgccaag gatgcggatc agtccgcgca tcagcttgtc cgggaaaaga
     3961 tgggcatcgc atttgaaaaa aatggcgccg agaccgttaa ccttcgtatg cggctccttt
     4021 tcctgcaatg ccaccgcatc gagcagatcc acgtccaaac ccagctcatg ccccttctca
     4081 acggtatgtc gcgcatgttc ggcccccgcc ggcgtctgga agatctctaa aagaccctta
     4141 tgctgataag cgaaatcgaa atgaggcagc ggaagccagg tgttctcata ctcatactgg
     4201 ctcaatacgg cgatgtcctt caatgggatc gccgaccgct ggacatgccc cggggtagcg
     4261 ctcttcatga acttcatccc ccagtcgacg agactgcggc taagcctcgg ttgcacatag
     4321 aacgggcttt gtgaattcag catccacttc aatcccggac gaacgatgcc cggggtagcc
     4381 agc
//
LOCUS       DASYSV010000619         6146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1408474, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000619 DASYSV010000000
VERSION     DASYSV010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408474"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VGQ51_19300"
     CDS             <1..212
                     /locus_tag="VGQ51_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038796.1"
                     /translation="AKEEERKATAKKGKGGPDQHLIQAYRNGQLFSRMVLDAYHGGLI
                     EPVEASHLLGTKINEFSKLEAFVYS"
     gene            209..706
                     /locus_tag="VGQ51_19305"
     CDS             209..706
                     /locus_tag="VGQ51_19305"
                     /inference="COORDINATES: protein motif:HMM:NF025725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HEV9038797.1"
                     /translation="MSANSQRYCLDANVLINAWTLYYHKDFCADYWKVLERLGHAGRI
                     FLADEVKKEIERQEDGLADWVKQSGLSVFESDGPITEIVSRIFLSDPRHVQLVDNIRG
                     RSLADPWVIAHAISERACVVTKEAKETQSEKRVRIPNVCEKMGVRCINDFQMIREIGI
                     RFSVV"
     gene            complement(735..1367)
                     /locus_tag="VGQ51_19310"
     CDS             complement(735..1367)
                     /locus_tag="VGQ51_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038798.1"
                     /translation="MEAPEQRTQSQISLSIELTDGAESIRKLYYAEGRGRILTFKPTK
                     QLLRYTLLFALLSESFYLISLKSDQISLVVLFVLSTLATLTLVMVFLRHAGKYFKWKK
                     SVKAMIKQADSYKKVSLIVNAQGFEARYDETVVIEKWSNFKRATLAPTHIFLFGDNAQ
                     NYLFPANAMEPSQYEAITQLARLGMGQSASSRNEQNQPAAEVGETPEIAE"
     gene            complement(1643..2155)
                     /locus_tag="VGQ51_19315"
     CDS             complement(1643..2155)
                     /locus_tag="VGQ51_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038799.1"
                     /translation="MILHKISLFAFSLAAVTLVCCNKQSGLGPNYTDYNPPAAPIVVT
                     NAVDYRPDPTVTTSLSGDSSITITMALTGNSGKSMTQITNVIGSTSYAAIQSATSKWY
                     NAAPITATNNSVTFKTSIGEYFKNYPLKAGTNPAATANVELAFRFYFSIELSDGSVVY
                     PTPVRVLVLQ"
     gene            complement(2183..3676)
                     /locus_tag="VGQ51_19320"
     CDS             complement(2183..3676)
                     /locus_tag="VGQ51_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEV9038800.1"
                     /translation="MKRPIKNIDRISRPLIILLFTGMLLGSCKKYLNVNTSPNAPTVV
                     PPSVLLPTTEIGIAFANSNDLDRATSAIMQQIAGVANQTLGYDVYNFSGAFDNQWNGE
                     LYGGSINNCVALINMDSATSPAYSGIAKLEMAYAFSMLTDIWGDVPYSQAGQGNTHLY
                     PRFDKVQDIYQGNSGLGIQSLFDLVNSGLADLAKPSTLKPKTDDIIYGGDLTKWTRMG
                     NTLLLKFAIMLTNVNPTMAKSVISSVISGNNYINSNSLDFEVPFGTSSSNQNPIYNFN
                     YVNRTGDQMLSSRFLALEQSLNDTLRLAKFFTKPNGVFTGFNNGSTAPAPTQATRSQY
                     GTYLIAPLSNPSALGAAPIRLLTNFQVDFILAEAALVLGTPGDPNQLYQAGITANMQK
                     IGMAASDITAYFVANPTVVTLSGTQQQMLQQIITQKYIAWCGNGIEVFDDYRRTGYPA
                     LSLPLNPGGDDASVIPTRLPYTNGELSTNPNAPNPRPLVDVKLWWAK"
     gene            complement(3694..>6146)
                     /locus_tag="VGQ51_19325"
     CDS             complement(3694..>6146)
                     /locus_tag="VGQ51_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9038801.1"
                     /translation="VLKGPAAAALYGSRAAAGAVIITTKKGGVNGRSEVVASSSYSRQ
                     NMEGLPQVQNLYGQGSTGIYNPASGNSWGPKFGATPSIANGLLVGGAAVPYKAYPNNI
                     NEFFQTGTISDSYLSISGGDAKQNFILSGGYLSQTGILPNTSVDRASVKFGGNTVFRN
                     KIKVGGSVNFINTLQTGILDGNGASSLAVVEGLARSIDLTTTRLKGTYQNPDGTNSWP
                     ITGQDNPYYDAYKNPLKSNVYRVIGSVNAGVDITSWLNVNYRLGIDAYTDRRKQIFAI
                     SSARVPAGQDLESDIVRSELNGDLIISAHKNDFLVKDLNISGLLGQNINQRRFQVVTA
                     QADALSVPGYYQINNGTNFAVGTAEQTTLQRLLGYYGQISLNWKDFLYLEATGRADES
                     STLSTSKSTYFYPSVNATFIFTDAFHIDSKIITYGKLRANISKVGTDAPPYQLANTFV
                     KGGYGNNVANFTFPDGSIAGFTLSSTIAPNLLTPEFVTSYEIGTNLGFWNNRVSLDAT
                     VYNTVSTSQIFSVGVPPSSGFTTKTVNAGKMTNKGIELTLSVEPVSTRNVRWDIVGNF
                     SRNINKVISIYPGITSFSIPGSAFIGSIPTIKVGQPYGVIVGGLIPRDSTTGARLINS
                     GTGVYATTIANQILANPNPEYILGVTNTIHYKTLSFGFTFNFIKGGQVLSFTAASYKS
                     RGAYVETGKDREKPWILPGEILANGKYTQNNIQIPAQTYWQTLGGLQSEFNVYDATVF
                     RLQDLTLGYDLPANIAKSMKINYIRISVFANNVFHVAPNAFFDPELNTQGAGNIRGLD
                     LQGTPNARTFGASLKVSL"
BASE COUNT         1394 a         1401 c         1774 g         1577 t
ORIGIN      
        1 cggccaagga agaagagagg aaggcaaccg cgaaaaaagg gaaaggcggc cctgatcagc
       61 acctaatcca agcttatcga aatggtcagt tatttagccg aatggtattg gatgcctatc
      121 atggggggct catcgagccg gtcgaggcaa gtcatttgct tggtacaaag attaacgaat
      181 tttcgaagct ggaagctttt gtatattcat gagcgcgaat agccaacgat attgcctgga
      241 cgccaatgtg ctcattaacg cctggacgtt atactatcac aaggattttt gtgcggacta
      301 ttggaaagta cttgagcgcc tgggtcatgc tggtaggata ttcctggccg atgaggtgaa
      361 gaaagagatc gagcggcagg aggatgggtt agcggattgg gtgaaacaaa gcggtctatc
      421 tgtttttgaa agtgacgggc cgatcacgga gatcgtcagt cgaattttcc tatcggatcc
      481 ccgccatgtg cagcttgttg ataatataag aggcaggtcg ctggcggacc cttgggtcat
      541 tgctcatgct atttcggaaa gggcctgtgt agtgacgaag gaggccaagg agacgcaaag
      601 cgaaaaacga gtcaggattc ccaatgtatg cgaaaagatg ggagtgcggt gtatcaatga
      661 ttttcaaatg ataagggaaa taggtattcg gttttctgtc gtctaagggc agagattggc
      721 gtacggatac tatcttactc cgctatttcg ggagtttcac caacctcggc agctggctga
      781 ttctgttcat ttcgtgatga tgcggactgt cccattccca gtctggcgag ttgcgttatc
      841 gcttcgtact gggagggctc catggcgttg gcgggaaaaa gatagttttg ggcgttatcg
      901 ccaaaaagaa aaatatgggt tggcgcaagg gtcgcccgtt tgaagttcga ccacttttcg
      961 atgacgaccg tttcgtcgta tctggcttcg aagccctgtg cgtttacgat gagactaact
     1021 tttttatagc tgtcggcttg ttttatcatc gccttcacgg actttttcca tttgaaatat
     1081 ttgccggcat ggcggaggaa caccatcacc aacgtcaacg tagcaagggt actaagaacg
     1141 aacaggacca cgagcgatat ttgatcggat ttcaacgaaa tgagataaaa cgactcggac
     1201 aagagcgcga aaaggagcgt gtagcgcaac aactgtttgg tcggtttaaa ggtgaggatg
     1261 cggcctcggc cctcggcata atagagtttt ctgatggact cagcgccatc cgtaagttca
     1321 atggatagag agatttggga ttgggtgcgt tgttcggggg cctccatttg ggtattttgg
     1381 cctaaactaa taaaaatcga tgactttatc aggggctgtc tcggttgcct tttagccaaa
     1441 ctggacagat ccctgggggg cgaagctgcc ctccgactaa gtatatttta caggtgatcg
     1501 ttttttctga tggggctttt tcacgtccgt attcaattga tcgattgaac accggtgcca
     1561 aagcggcggc aactcaagca acataacaaa accgccccga tattccgggg cggtctattt
     1621 cagatggcag agattgccac ttttattgta aaaccagcac tctcaccggc gtgggatata
     1681 ccacactgcc atcgctgagc tcgatagaga aataaaaccg gaatgcaagc tcgacattgg
     1741 ccgtagcagc aggattcgta cctgccttta gcggataatt cttaaagtac tctccaattg
     1801 aagttttaaa agtcaccgag ttatttgtcg cggttatcgg agccgcattg taccatttcg
     1861 atgtagccga ttgtattgcg gcatagctgg tcgatccgat cacgttggtg atctgcgtca
     1921 tagatttgcc actgttgccg gtcagcgcca tggtgatcgt gatgctgctg tcaccgctga
     1981 gcgaagtcgt gactgtcggg tcgggacggt aatctacggc attggttacg acaatgggcg
     2041 ccgccggcgg attgtaatcc gtgtaattcg ggcccaggcc actctgtttg ttgcagcata
     2101 ccagagttac tgctgcgagg gaaaacgcga atagcgagat tttatggagg atcatatttt
     2161 tttcttttag tatggataaa atttatttgg cccaccagag ctttacgtct accagcggtc
     2221 tcggattggg cgcgttggga ttggtggaaa gttctccatt ggtataaggc aaccgggtag
     2281 ggatcacgct ggcgtcatcg cctccgggat tcaacggcag cgacagagca ggatatcccg
     2341 tcctgcggta gtcgtcgaat acctcgatgc catttccgca ccatgcgata tatttctggg
     2401 tgatgatctg ttgcaacatc tgttgctgcg taccgctgag cgtgacgacg gtagggttcg
     2461 ctacaaagta cgcagtgata tcggatgctg ccatcccgat cttttgcatg ttggccgtga
     2521 tgcctgcctg gtagagctgg ttcggatcgc ccggggtccc gagtacaagc gccgcctctg
     2581 ccaggatgaa atcgacctgg aaattggtca gcaaacggat gggggccgca ccaagggcgg
     2641 atggattgga taacggcgct atcagatagg tgccatattg cgaccgggtc gcctgtgtcg
     2701 gtgcgggggc ggtagaaccg ttgttgaagc ccgtgaagac cccattcggt ttggtaaaga
     2761 atttcgctaa ccgcaaggta tcgttcaggc tttgctccag cgccaggaaa cgagagctca
     2821 gcatctggtc accggtgcgg ttgacatagt tgaagttata gatcgggttt tggttactgc
     2881 tggaggtgcc gaaagggact tcgaaatcca aagagttaga attgatgtag ttgttcccgc
     2941 tgatcacgct gctgatcacg ctcttggcca tcgtagggtt gacgttggtg agcatgattg
     3001 caaacttgag gaggagggtg tttcccattc gcgtccattt cgtcaggtca ccgccataga
     3061 tgatatcatc agtcttcggt ttgagtgtgc tgggtttcgc cagatcggcc agaccgctgt
     3121 tcacgaggtc gaacaggctt tggatgccca ggccggaatt gccctggtag atgtcctgta
     3181 ctttgtcaaa acgggggtag aggtgggtat ttccctgacc cgcctgagaa tagggtacat
     3241 ctccccagat gtcggtaagc atagagaagg cataggccat ctccaatttg gctatgccgg
     3301 aataagcagg gctggtggct gaatccatat taatgagcgc gacgcagtta ttgatactgc
     3361 cgccgtatag ttccccgttc cattggttgt cgaatgcgcc gctgaaattg tagacatcat
     3421 agccgagggt ctgatttgcc acaccggcga tctgctgcat gatcgcggac gtcgcacggt
     3481 cgagatcatt actgttggca aaggcaatac cgatctccgt agtgggtagc aatacggatg
     3541 gcggcactac ggtcggcgcg tttggagaag tatttacatt caggtatttt ttgcaagaac
     3601 ccaatagcat ccccgtgaac aggaggatga tcaagggccg gctgatccga tcgatatttt
     3661 taattggtcg tttcatgttc tgtttttttt acgttagaga gaaactttga gacttgcacc
     3721 gaatgtccgg gcattagggg taccctggag atccagaccg cggatgttac ccgcaccttg
     3781 cgtgttgagc tccgggtcga agaatgcatt tggcgcgaca tggaacacgt tgttggcaaa
     3841 gacggagatc cggatataat ttatcttcat gcttttggcg atattggccg gaagatcgta
     3901 gcccagggta aggtcttgca gacggaacac ggtagcgtca tatacgttga attcactttg
     3961 caaaccaccc agcgtctgcc agtaggtttg cgccggaatc tggatgttgt tctgggtata
     4021 tttcccgttg gcgaggatct cgccgggaag tatccagggt ttctcacggt ccttgcctgt
     4081 ctcgacatag gcgcccctgg atttatacga agcagcagtg aaggagagca cctggccgcc
     4141 tttgataaaa ttaaaagtaa atccaaagga gagcgtctta taatggatag tgtttgttac
     4201 acccaatata tattcaggat tggggtttgc caggatctga ttggctatcg tcgttgcgta
     4261 tactcctgtt ccggaattga tgagacgggc gcctgtcgtg ctgtcacgcg ggatcaggcc
     4321 gccaactatc acaccgtagg gttgtccgac cttgatggtg gggatcgatc cgataaaggc
     4381 gctgccaggt attgaaaagc tcgtgattcc cggatagata ctgataacct tgttgatatt
     4441 ccttgagaaa ttgccgacga tatcccaccg gacattcctg gtggagacgg gttcgaccga
     4501 gagcgtcagc tctatccctt tgttagtcat cttacctgcg ttgaccgtct tggtggtaaa
     4561 gccgctggaa ggtggaacac cgacggagaa gatctggctc gtgctgacgg tattgtaaac
     4621 ggtagcgtcg agtgaaacac ggttgttcca gaagcccagg ttggtgccta tctcatacga
     4681 tgtcacgaat tccggcgtga ggagattggg tgcgatggtg gagctcaagg tgaatcctgc
     4741 gatggaacca tcggggaagg tgaaattggc tacgttgttt ccgtaccccc ctttcacgaa
     4801 agtattggcc aactggtaag gtggagcgtc agtgcccact ttggagatat tggcgcgaag
     4861 cttgccataa gtgatgatct ttgaatcgat atggaaagcg tccgtgaaga tgaacgtcgc
     4921 attgacggag ggatagaaat aggtgctctt gctcgtggac agtgtggaag attcatccgc
     4981 gcgaccggtt gcttcaaggt atagaaaatc cttccagttc agggatatct ggccatagta
     5041 acctaacaga cgctggagtg tcgtctgctc tgctgtcccg acggcgaagt tggtgccatt
     5101 gttaatttga tagtaacccg ggacggacag cgcatcggcc tgtgccgtca ccacctggaa
     5161 acggcgctgg ttgatattct gacccagcag gccgctgatg ttgaggtcct tgaccagaaa
     5221 atcgttctta tgtgcggaga tgatcagatc gccgttgagc tcgctgcgga cgatatcgct
     5281 ttccaggtcc tggccggcag ggacgcgggc agaactgatg gcgaagatct gttttctcct
     5341 gtcggtatag gcgtcgatcc ccaaccggta attgacgttc agccaggagg tgatatcgac
     5401 accggcattg acgcttccga tgacgcgata aacattgctt ttgagtgggt tcttatatgc
     5461 atcgtagtaa gggttgtcct gacccgtgat cggccaacta ttcgtgccgt cgggattctg
     5521 ataggtgccc tttagccgtg tggtcgtgag atcgatgctg cgagcgagcc cttcgacaac
     5581 ggcgagcgag ctggctccgt tgccgtcgag gataccagtc tgcaaggtat tgatgaaatt
     5641 aaccgaaccc ccgaccttta tcttattcct gaaaacggtg ttgccgccga atttgacaga
     5701 ggccctgtcg actgacgtat tgggcaggat gccggtctgt gagagataac cgcccgacag
     5761 aatgaaattc tgcttcgcat cgcctccgct gatgctgaga tagctgtcag agatcgttcc
     5821 cgtctggaag aattcgttga tattattggg atatgccttg taaggtacgg ccgctccgcc
     5881 gacgagcagg ccgttagcta tcgatggagt agctccgaat ttcgggcccc aggagttgcc
     5941 ggacgccggg ttatagatgc ccgttgagcc ttgtccataa aggttttgca cctgcggcag
     6001 gccttccatg ttctgcctgc tgtacgagct gctggcaacg acctcactcc ttccattgac
     6061 accacctttt ttggtggtga tgatgacggc gccggcggct gctctcgaac catacagcgc
     6121 tgcggcggcg gggcctttca ggacgt
//
LOCUS       DASYSV010000620        16532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1279098, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000620 DASYSV010000000
VERSION     DASYSV010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 16532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16532
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279098"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /locus_tag="VGQ51_19330"
     CDS             <1..1983
                     /locus_tag="VGQ51_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038802.1"
                     /translation="RNTSSLLDGRLTLDLGAAYINVGEQNMLSQGQYFNPLIPIYLFP
                     RGDNIEKYKVYERYDATRNFKTQFWPYGDEGFQMQNPYWIINRDFFINNKDRYLLTAA
                     AKYNINSWINITGRVKLDDNTTIGEKKYYASTAGLFASPAGAYYRATAITKQTYADIL
                     VNIDKTLTRSLRLTANVGSSYLDTKYNADSYGGNLLSVPNLFSFTNVNQTQATVMQDS
                     YHDQSQAVFGTAELSWRNMAFLDLTGRNDWSSALVNTTASSIFYPSVGASAVLSDLLH
                     MHSQTLSFLKVRGSYSEVGNPPQRFITIESYPIQGGFPVTSSYLPATGLQPERTKSTE
                     AGVNMKFFRNHLSLDVTAYQSNTYNQLFNPVLAPSTGYSSFYINAGQVTNKGIEAALG
                     FNGRIARGLDWSSTATFTLNRNKIVKLLTNYNDHGTGQTVTVDSLSVGGTGSYMMSLV
                     RGGSIGDIYVTTLETDEHGHIFVDAASKTVLADPNNTYVKAGNANPRYNIGFRNSFTY
                     RNFNFSFLVAARIGGVGVSVTQAIMDRFGVSKTSADARNAGGVKVNGYLIPAQPYYQV
                     VGGGVAAIGSMYVYSATNVRLGEASLAYDIPASVFRNKIQGVTLALTGRNLFMFYNKA
                     PFDPESTANTDTYWQGIDYFMQPSLRSVGFSVKVKL"
     gene            2004..3617
                     /locus_tag="VGQ51_19335"
     CDS             2004..3617
                     /locus_tag="VGQ51_19335"
                     /inference="COORDINATES: protein motif:HMM:NF024152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9038803.1"
                     /translation="METNKWKFFRKSLLPGLALVLCFGCTKNFREYNTNPNAATDAQL
                     QYDNLATGVFFSQMEQNIFPTAQEPAFGDEMYQIVQNLCGDVYSGYMGASNNWFGGVN
                     STNYAMTPSWYGQAFNRAFLSVMPAWLSIRKKTTGNPAMQHIYALAQIIKVESISRTT
                     DMYGPLPYTQFGSGSLSTPYDPQSTIYYSFFTDLDSAIATLTSYVQNNPGSMPLANYD
                     LIYGGDYTKWIKFANSLKLRLAMRLAYVDPAKAQQYAEAAVANSYGVFTTDADDALLA
                     SADGINIHNPLQIICYNFGDVRMGANMESFLTGYKDPRLPFLFNPAVTDGKYHGIRNG
                     ITITNKSLYTDPFSTLNVQPTTPIRWMSAAEMYFLRAEGAIRGWNMGGSAQSLYETGI
                     QTSFNQWGAGSAANYILDSTDKAAPYSDPSVNSNNMTTGLSTITIHWKATDPMETQLE
                     RIITQKWIATYPDGQEAWSEFRRTRYPKIFPVAINNSAGLINTTTQIRRLPFPLTEYQ
                     SNAAGVATGVSELKGGDNGGTKLWWDAKP"
     gene            3629..4672
                     /locus_tag="VGQ51_19340"
     CDS             3629..4672
                     /locus_tag="VGQ51_19340"
                     /inference="COORDINATES: protein motif:HMM:NF027467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18"
                     /protein_id="HEV9038804.1"
                     /translation="MTKKFISPFLFLTISIGLVNCKKQTTPVALNVQQPTEHSAEYYE
                     NLRAYKNSPHQIFYGWFAAYGNAEGVVADYKQSASWGEHIAGLPDSLDFCSLWSGIPS
                     LKQGDSTSTYNPIAYNEMRSSMAQRGIKFVIPEICRIQKYNGKFTLDSAGMRAYAGYL
                     LGMVFNYDLNGLDLDYEPNNGSFVDWLSGNNFAQFVQMLGDSIGPMSKHPDKYLIIDY
                     YTDAIPSSVEPYINYLVNQSYTQGATTTSASFLQSRYNAVSFCPTRKFIVTENIGNWW
                     QTGGAPFTEADGNTLTAEGTQMYSLEGFARWNPTQGQKAGWGGYYFDRDYNNSPPYYY
                     VRRSIQIVNPSVH"
     gene            4688..5851
                     /locus_tag="VGQ51_19345"
     CDS             4688..5851
                     /locus_tag="VGQ51_19345"
                     /inference="COORDINATES: protein motif:HMM:NF020111.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 and LamG domain-containing protein"
                     /protein_id="HEV9038805.1"
                     /translation="MNRKYNFLFLLIAVIIVTPIGFSCRKYRQFNDVILITGTETDPL
                     IKFTVENTPSSINVTASSTGLAAKNITVNFAVDTALVSTYNTQVKGNYFAAPANAYTL
                     SGGSGVIHSGTNVSDPVTVTLVSTDSLKDGRNYLIPITIKSVNGPESILETSRTIFLK
                     IARVTQFNSIDISSPNFYDIDTFTTPIQNISQFTFEIKCFLNSWHTGNPPISRLCNWG
                     PQDQSTFNLLRFGEAGSDTNQLQWINSAGGMFSNTRFALKTWYTISCVCDGSSYRMYV
                     NGELDNSFDASGQTYTWGALELGMSYAGYQTAQRFLGRMAEIRFWSRPLSQTEIQQNI
                     CAVDPTAPGLVSYWKLNEGSGQAFTDLTGNGRNITWRKAPVVWNADSVNKCAQ"
     gene            5874..6863
                     /locus_tag="VGQ51_19350"
     CDS             5874..6863
                     /locus_tag="VGQ51_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 domain-containing protein"
                     /protein_id="HEV9038806.1"
                     /translation="MLRYIRLSPILLLLLAGSCKKNNGLDSKDLLVYMQGDFGDANNA
                     ITAELTLTPIAVWGNTVFQFPVYSTRQMAAAANVTITPDSSGVAAFNKKTGSSCILLP
                     SNTYTIDITQLTIHTDSMQSGPLTVTITNPAALTDTNGYVLPLTITKVTGKDEGVAIS
                     SNRATAYLYIPYAYTNVDTVQTLLSGTIADRTGWMVTVSNTTSGSLGPAMLDGSNSTA
                     WRSSNSSTAAKWVVLSLPVAQTVRGLQLTPDYVSTGDNATRIRVSSSPDSVTWSVQGI
                     WNGTGPASGTNATNPDFKGINFLSPVQARYFRLDILKWVSGSRTGIGELNAIQ"
     gene            6997..9528
                     /locus_tag="VGQ51_19355"
     CDS             6997..9528
                     /locus_tag="VGQ51_19355"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9038807.1"
                     /translation="MKQSILFLSMLLVIGQVFAQAPPNSGAQTGRLYGKIVDSAGHGI
                     GRASVLILKAGTGPATGKKKQVLVKGTITQNNGDFSAEDLPMGSPLQLNVSAVGYTAL
                     QQAVVLTRQAADKDLGSVVMLPDTKQLEQVVVTASKPSMTLDMDKKVFNVSKDIVSAG
                     GNGLDVLKNVPSVNVDIDGNITLRGGSPQLFVDGKPTTLTLDEIPSDAIESVEVITNP
                     SAKYDASGGGAGILNIVLKKNRKTGYNGSVRAGADVYGGLNGGSGLNVRQGKINFSAD
                     LNFRQGRDRGTSSIQRLDYSTTPNTDLNQNVLDTSKNTMYFGRIGLDYFLNNRTTVSL
                     TGFAMHHGGMQTSDITMNTDSLFTAGKISQNSREEINGDRSFNGRGATLGIKRLFAKD
                     KEEWTADASYFSGNASNNSIYSTDYYQYGTASTLTGSTLQKIAGGGNDHNLILQSDFS
                     DPLTPKTTLEAGVRAALQGRLNINNNYTWNADSAAYEPVPSAASNYESRSNVYAAYVT
                     LGSSIGNFTYKAGLRAESSNYHGTLLNSGQSFANQYPISLFPSLFLGQKIGDDQELQL
                     SYTRRINRPNFFQLVPYTDSSNKLNITRGNPDLVPEFTQSLELSWLKTFSRNSTLLAS
                     VYYKHTDHLITGYIEADTNASTGAVSFINTFVNAESSNSLGAELTGQFPLTRWWDVSA
                     NVNLYHSQIDIGGSDAVAQPAMWSWFGKMNTNFRLPSGFAVQVSGMYQSKTNLPVNTS
                     AGQPGPPNMQSQSASQGFIRPYYDIDLALRKTLLQGKMIASLSFNDVFRSRKQDQYTF
                     STWFTQDYSRLRNPQMLRLSLTWNFGKVDASLFKRKNNNVQAEDQ"
     gene            9578..10615
                     /locus_tag="VGQ51_19360"
     CDS             9578..10615
                     /locus_tag="VGQ51_19360"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9038808.1"
                     /translation="MKLRYNRRAIRIVTHVAAWAVALSLPYLLDSHHGVRHHKDDIHE
                     WDFFYLNFITSILWIGPFYLNAYLLMPAFFYRKRYLAYTALLAASFFSVFLINLLLFV
                     YAFGLPHYNPQGAIGFLLPTFILFIAVSTAYRVVRDKTEADELAHQRHEENLKTELSF
                     LRSQINPHFIFNILNNLVALEQMRSPELGPTILKLSALMQYMLYETDEEKVPVNKEIE
                     YLQCYIDLQRQRFGAKVPVSLSLDQTPSIYEIEPMLLIPFVENAFKHGVGMIEHPAIH
                     VSLHIEDGVLLFSVRNRYNPSSTETRDKGSGIGLGNVSRRLRLLYGNQQVLQISREED
                     WFIVKLELNLH"
     gene            10615..11340
                     /locus_tag="VGQ51_19365"
     CDS             10615..11340
                     /locus_tag="VGQ51_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV9038809.1"
                     /translation="MLRCIAVDDEPLALHLLTDYIRKVPFLDLVASCEDAFQAARVLQ
                     ENQVDLIFMDIQMPGLTGLQFIQSLAKRPMIILVTAYKKFATEGFDLDVVDYLVKPVG
                     LDRFMKACYKAQELHQLRTAPPATPAGATGAAAPDYFFVNSDYSLVKVVFADIVWIEG
                     SGDYVKIHLHSAPRPLLVRTNAKTLEGELPADKFLRIHKSYIIAIAAITAIRKNSVFI
                     GELELPVGDTYRETLRRITGREL"
     gene            11368..12522
                     /locus_tag="VGQ51_19370"
     CDS             11368..12522
                     /locus_tag="VGQ51_19370"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV9038810.1"
                     /translation="MTANSTYLESKSHYQILDGLRGVASIIVVVFHVFETFNGGSRFA
                     QLINHGYLAVDFFFVLSGFVVAYAYDDRWGKMTKWDFYKRRLVRLQPMVVMGSLIGAA
                     LFYFQASPAFPLVAGTPVWVMLLVMLVGCTLLPLPISMDIRGWQEMHPLDGPAWSLFF
                     EYIANFLYAVVIRRLNKLALSIFVVLAACLLIHYTVMGPQGDLIGGWALNGEQLNIGF
                     TRVLYPFFAGVLLCRIGKLPQVNGAFWICTLLIAIVLGIPRIGGPEHLWMNGIYESAV
                     VIFVFPFIVWLGAGGTIRNKRSVRICRFLGDISYPMYITHYPLIYVFTAWVSKNKIPM
                     SKGLLPGALLVVASVAIAYACLKLYDEPVREWLKERVLKKSKPEVQAAIN"
     gene            complement(12526..13233)
                     /locus_tag="VGQ51_19375"
     CDS             complement(12526..13233)
                     /locus_tag="VGQ51_19375"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEV9038811.1"
                     /translation="MIRKLLTGRPRREGGFRHRGHEIQRIETFSDAVFAFAITLLIVS
                     LEVPKTFDELLTSIRGFVAFGICFTFLFIIWNEQHVFFRRYGMEDKTTLFLNAVLLFI
                     VLFYVYPLKFLFSLLLGGNTGGAVRPIREEQIPQLMIIYSVGYIVIYVVFLLLYLHAF
                     RRRGRLELTNLEVFDTRSRIWSHAIQIIIGATSMLTALILPARVGGLAGWVYPLIGPV
                     LWIFYARRARRRWKIGR"
     gene            complement(13272..14747)
                     /locus_tag="VGQ51_19380"
     CDS             complement(13272..14747)
                     /locus_tag="VGQ51_19380"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV9038812.1"
                     /translation="MLPQRQNNAGPRPLPLEDYFSSFADHVIGRRQFFESPFGRNRVI
                     YADWTAGGRGYGPIEKRLQCNVLPFFANTHTGTTVTGRAMTAAYEEAKAIIKAHVHAD
                     TDDLLLFCGSGMTDAVCRLQRMLGLRASKEEIVVFVTHMEHHSNHISWLETGATVEII
                     RAAPDGNVDLEYFTELLKKYKHRRVKIAAVTACSNVTGIQTPYHTIAALVHAYSGYCF
                     VDFASSAPYVSIDMHPPEPGGDLDGIYFSCHKFLGGPGTPGVLVFNGRLHKGGVPDRP
                     GGGTILYSNPWGGREYISDIEQREDGGTPPILQAIKAALCIRLKEEMGVEQMLRREEE
                     LLECVFDRLPGMPGVTILEAGYRRRLGIVSFMMTGGHYNLVVKMLNDRFGIQTRGGCS
                     CAGPYGHFLLHIDEARSQAMRASLKSGDLSCKPGWVRLSLHPTMTDDEIGYILDAIGI
                     TVERYREWAADYVYDPASNEFVFRGWGERNRVREWFSAPLR"
     gene            complement(14725..15387)
                     /locus_tag="VGQ51_19385"
     CDS             complement(14725..15387)
                     /locus_tag="VGQ51_19385"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HEV9038813.1"
                     /translation="MGNIIQFGENVPGYSVRVLNEREIRAAAGILFLATFISLNIILA
                     TGNFVPVKYVVTIFLLDFIIRVFVNPRYSPTLIIGRWIVRRQTPEYVGAPQKKFAWAI
                     GVALSAVMFVFLVLVNAYSPITGLTCLVCLIFLFFETSFGICLGCKFYPLFFKDRAQY
                     CPGEVCDVRSKQDIQKTSGGQWLVVAGLVVFVVVLATFFNGAFGRAPHDLFNSHATAK
                     TK"
     gene            15549..>16532
                     /locus_tag="VGQ51_19390"
     CDS             15549..>16532
                     /locus_tag="VGQ51_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV9038814.1"
                     /translation="MRSLLDIRRFKLYGWVGLGIVCMCTIQEIHSEHRGFFIAILDCL
                     WLTIWLTLVNYLLYEYAVPRLRWRKFYIGILLFIFYLFLSTEGFHLWAQLGYALHVNV
                     DYIETAGKRHHLSEEASFSLPSSIMFGIAGVIFNNVRLRQATQQLRIEKQQAELNYLK
                     SQTNPHFLFNTLNNIYALARDRSDLAPESIMRLSKILRYMLYDAGGQYVAIEQELKVI
                     GDYISLEQLRYDDTLRVNFNYDVEDMKQSLPPLLLVPMVENAFKHGASETRNRPFVDI
                     HLSIASRQLAFLVRNSIEGQPDEGSIKENIGLSNLRRQLELLYTDYRLNVEH"
BASE COUNT         3913 a         4713 c         4263 g         3643 t
ORIGIN      
        1 cgaaatacct cttcgctgct tgacggtagg ctgaccctcg atctgggcgc cgcctatatc
       61 aatgtgggtg agcagaacat gttgtcgcag gggcaatatt tcaacccact gatcccgatc
      121 tatctttttc cccgtggcga caacatcgaa aaatacaagg tctacgagcg gtatgacgcg
      181 acgcggaatt tcaaaacaca gttctggcct tacggcgacg aaggattcca gatgcagaat
      241 ccttactgga tcatcaaccg ggattttttt atcaacaata aggaccggta tctcctcacc
      301 gccgccgcca aatacaatat caacagctgg atcaatatca ccggccgggt aaaactggat
      361 gacaatacca cgatcggcga gaaaaaatat tacgcctcca cggctggtct ttttgcctct
      421 cccgccggcg cctattacag ggcaacggcc atcaccaaac aaacgtatgc ggatatcctc
      481 gtcaatatcg ataagacgct gacacgcagt ctccggctga ccgccaacgt cgggagcagc
      541 tacctggata ccaaatacaa tgcggacagt tatggcggca acctgctgag cgttcccaac
      601 ctgttcagtt tcaccaacgt caaccagaca caggcgaccg tcatgcagga cagctatcac
      661 gatcagtcgc aggcggtatt cggtaccgcc gagctctctt ggaggaacat ggccttcctc
      721 gatcttaccg gaaggaatga ctggtcctcc gcgctcgtca atacgacggc cagttctatc
      781 ttttacccgt ccgtcggcgc ctccgcagtc ctgagcgatc tgctgcatat gcattcgcaa
      841 acgttgtcct tcctgaaggt ccgcggttct tacagcgaag taggtaaccc gccacagcgt
      901 ttcatcacca tcgagagcta cccgatccag ggcggcttcc cggtgaccag cagctattta
      961 ccggcaacag gtctccagcc tgagcggacg aaatccaccg aagccggtgt caatatgaaa
     1021 ttcttccgga accacctgag cctcgatgtg acggcctacc agtcgaatac ctacaaccag
     1081 cttttcaacc ccgtactggc cccgagtaca ggctactcca gcttttacat caatgccgga
     1141 caggtaacca ataagggtat cgaggcggcg ctgggcttca acggccgcat cgcgcgtggg
     1201 ctcgactggt cctccacggc tactttcacg ctcaaccgga acaagatcgt caaattgctg
     1261 accaactaca atgaccatgg tacgggtcag acggtcacgg tcgacagcct cagcgtaggc
     1321 ggcacgggca gctatatgat gtcgttggtc aggggcgggt ccatcggcga tatctatgtc
     1381 accacgctgg agacggatga acacggacat atcttcgtcg atgcggcttc caaaaccgta
     1441 ctggccgatc ccaacaatac ctatgtcaag gcgggtaatg ccaacccccg ctacaacatt
     1501 ggtttcagga acagctttac ctacaggaat ttcaatttca gctttctcgt cgccgcccgt
     1561 atcgggggtg taggcgtctc cgttacccag gccatcatgg accggtttgg cgtatcgaag
     1621 acctccgccg acgcgcgcaa tgccggcggt gtcaaggtca acggatatct catcccggcg
     1681 caaccctatt accaggtggt gggcggcggc gtagccgcca tcggctccat gtatgtgtac
     1741 agcgccacta atgtccgttt gggcgaggcc tcgctggcct acgacatccc ggcatctgta
     1801 ttccgcaata agatacaggg cgtgacgctg gcgctgacag gcaggaatct gtttatgttc
     1861 tacaataagg cccccttcga tccggaatcg accgcaaaca cggacacgta ctggcagggc
     1921 atcgattatt tcatgcagcc cagtctgcgc agtgtcgggt tcagcgtgaa ggtgaaactc
     1981 taattaacaa aagcattatg atcatggaga ccaataaatg gaaattcttt cgaaaaagcc
     2041 tgctgcctgg tctggcactc gtgctgtgtt tcgggtgtac caagaacttc agggaataca
     2101 atacgaatcc caacgcagcg accgacgcgc agttgcaata tgacaatctc gccacgggtg
     2161 tgttcttttc ccagatggag cagaacatct ttcctacggc gcaggaaccc gccttcggcg
     2221 atgagatgta ccagatcgtg cagaacctct gcggcgacgt ctattcgggt tatatgggtg
     2281 ccagcaacaa ctggttcggc ggcgtcaaca gcaccaatta tgcgatgacc cctagttggt
     2341 acggtcaggc attcaaccgg gccttcctca gcgtcatgcc ggcatggctg tcgatcagga
     2401 aaaagacgac cggcaatcct gccatgcagc atatctatgc attggcgcag atcatcaagg
     2461 tcgaatcgat ctcgagaact acagatatgt atggcccgtt gccttatacg cagttcgggt
     2521 cgggaagcct gtcgacgcct tatgacccgc aaagcaccat ctattattcc ttttttacag
     2581 acctggactc ggcgatcgcc acgctcacga gttatgttca gaataatccg gggtcgatgc
     2641 cgctggccaa ttatgatctc atctatggcg gcgattatac caaatggatc aagttcgcca
     2701 actcgctgaa gctgcggctc gctatgcgct tagcctatgt ggaccccgca aaggcgcagc
     2761 aatatgccga ggcggctgtt gccaactctt atggcgtatt cacgacggat gccgacgacg
     2821 cgttgctggc ttcggccgac gggatcaata ttcacaaccc actgcagatc atctgctaca
     2881 atttcggcga tgtccggatg ggggccaata tggaatcctt ccttaccggc tacaaagacc
     2941 cgcggcttcc gttccttttc aatccagcgg tcacggacgg caaatatcat ggcatccgca
     3001 acggcatcac gatcaccaat aagtccttat atacggaccc cttctctacc ctgaatgtac
     3061 aaccgacgac gcccatccga tggatgtcgg ccgccgagat gtatttcctc cgggccgaag
     3121 gcgctatccg cggctggaat atgggaggct cggcccagtc gttgtacgag acgggcatac
     3181 agacctcctt caaccaatgg ggggcaggct ccgctgccaa ctatatcctg gattccaccg
     3241 acaaggcggc tccatactcc gatccttcgg tcaacagtaa caacatgact accggtctca
     3301 gcaccatcac cattcactgg aaggctaccg accccatgga gacacagttg gaaaggatca
     3361 tcacccagaa atggatcgca acataccccg acggccagga ggcctggagc gagttcaggc
     3421 gcacccgcta tccgaagata ttccccgtcg cgatcaacaa cagtgcgggc ctgatcaata
     3481 cgaccacgca aatcaggaga ctaccgttcc ccctgacgga atatcaaagc aatgcggccg
     3541 gagtggcaac tggcgtttcc gaactcaaag gcggcgataa tggaggcacc aaactttggt
     3601 gggacgctaa accttaaaaa ttcagatcat gacaaaaaaa ttcatatcac cctttctctt
     3661 tctgacgatc agcatcgggc tggtcaactg taaaaaacag accacgccgg ttgcgctgaa
     3721 tgtccagcaa ccgacggaac acagcgccga gtactacgag aacctccggg cctataagaa
     3781 ttctccgcac cagatcttct acggctggtt tgcagcctat ggcaatgcag agggagtggt
     3841 agccgattat aaacagtctg cctcctgggg cgaacatatc gcgggtctac ccgacagcct
     3901 cgatttctgc agcctttggt caggcattcc ttcgctgaaa cagggcgact ccaccagcac
     3961 ctataaccct atcgcctata acgaaatgcg gtcttccatg gcccagcgcg ggatcaagtt
     4021 cgtcattccg gagatctgcc gcatccagaa gtataacggc aagttcacgc tcgactctgc
     4081 gggaatgcgg gcgtatgccg gctatctgct aggtatggtc ttcaattatg accttaacgg
     4141 gctcgatctc gactatgaac ccaacaacgg ttcgttcgtc gattggctat ccgggaacaa
     4201 tttcgcgcag ttcgttcaaa tgctgggcga ttccatcggt ccgatgtcga aacacccgga
     4261 caaatacctg atcatcgatt actataccga tgctatccct tccagtgtag aaccttatat
     4321 caactacctg gtcaaccagt cctatacaca aggtgcgacc actacatcgg catcgttcct
     4381 ccaatcgcga tacaacgcgg tgagcttctg cccgacgcgg aaattcatcg tgacggagaa
     4441 catcggcaac tggtggcaaa ccggcggcgc ccccttcacg gaggccgacg gcaataccct
     4501 gacggccgaa ggcactcaaa tgtattcgct ggaaggcttt gcccgctgga atcctacaca
     4561 gggacaaaag gccggctggg gcggatatta ttttgaccgg gattataaca actcaccgcc
     4621 ttactactat gtccggcgga gcatccagat cgtaaatcct tctgtgcatt aattcaaact
     4681 ttcaactatg aaccgtaaat ataatttcct ctttctgctg atcgcggtga tcatcgtcac
     4741 cccgatcggc ttctcctgcc ggaaatacag acaattcaat gatgtgatcc tgatcacagg
     4801 caccgagaca gaccctctga tcaagttcac cgtggagaat acgccttcca gcatcaacgt
     4861 cactgccagt tctaccggtc tggccgccaa aaatatcacg gtgaacttcg cagtggatac
     4921 ggcgcttgtc tcgacctaca atacgcaggt aaaaggcaac tatttcgcgg cgcctgccaa
     4981 tgcctatacc ctgtccggcg gcagcggcgt catccattcg ggcactaatg tctccgatcc
     5041 ggtgacggtg acccttgtct ctacggacag cctgaaggat ggcaggaatt atctgatacc
     5101 gatcaccatc aagagcgtca acggtccgga gtcgatattg gagacttcgc gaaccatctt
     5161 tctgaagatc gcgcgggtga cccaattcaa ttcgatcgat atctcgagtc ccaattttta
     5221 tgatatcgac acgttcacga ccccaattca gaatatctcg cagttcacct tcgagatcaa
     5281 gtgtttcctc aactcctggc atacgggcaa tccgcctatc agcaggctgt gcaactgggg
     5341 tccgcaggat cagagtacat ttaatttgct gcgttttggc gaagcgggca gcgataccaa
     5401 ccagctgcaa tggatcaatt ccgcgggcgg gatgttctcc aatacaaggt tcgcgctgaa
     5461 gacgtggtat acgatctcct gcgtctgtga cggcagctcg taccggatgt atgtgaatgg
     5521 ggagctcgac aacagcttcg atgcttcggg tcagacctat acctgggggg cgctcgagtt
     5581 ggggatgtct tatgcgggtt atcagacggc acagcgcttt ctcggcagga tggcggagat
     5641 ccggttctgg agccggccgc tttcgcaaac ggagatccag cagaatatct gtgcggtcga
     5701 tcctaccgcg cctggcctgg tgtcttactg gaagctcaac gaagggtcgg gtcaggcctt
     5761 taccgacctc accggcaacg ggcggaatat cacctggcga aaggcacccg tagtctggaa
     5821 tgcggacagc gtcaataaat gcgcacaata gttcaactac ttaaaaaaat actatgctga
     5881 gatatattcg actatcgccc atccttttgc tgttgctggc cggctcctgc aaaaagaaca
     5941 acgggctgga ttccaaggac ctgctggtct acatgcaggg agacttcggt gacgcaaaca
     6001 atgcgatcac cgcggaattg acgctgacgc cgatcgccgt ctggggcaat acggtctttc
     6061 aattcccggt ctattcgacc cgacaaatgg cggctgcggc caatgtcacg atcacgcccg
     6121 actcgagtgg cgtagcggcc ttcaataaga agacggggag cagctgtatt ctcctgcctt
     6181 caaataccta taccatcgat attacccagc tgaccataca tacggattcg atgcagtcgg
     6241 ggccgttgac ggtcaccatc accaacccgg cagcattgac agacacgaat ggttatgttc
     6301 ttccactgac gatcacaaag gtcaccggca aggacgaggg cgtcgccatc agcagcaaca
     6361 gggccaccgc ctacctgtat attccatatg cctataccaa tgtggacact gtacagacct
     6421 tactttccgg cactatagcc gaccgtacgg gctggatggt aacggtaagc aatacaacct
     6481 cgggatccct cgggccagcg atgctggacg gaagcaatag tactgcatgg cggtccagca
     6541 atagtagcac tgctgccaag tgggtagtat tgagtttacc cgtggcgcag acagtccgcg
     6601 ggctgcagct gactccggat tatgtgagca ccggcgacaa tgctacgcgg atcagggtgt
     6661 caagcagtcc ggatagcgtc acctggagtg tgcagggtat ctggaatgga acggggcccg
     6721 cgtcgggtac caatgccacg aatcccgatt tcaaggggat aaatttcctg tcgcctgtgc
     6781 aggcaaggta tttccggttg gatattttga agtgggtcag cggcagcagg acaggtatcg
     6841 gcgaactgaa tgccattcaa tagattagga ccttatactc ctgaaacccc atggccccgg
     6901 gcttcgcccg gggccttttt ttccggttca ccgcatcttt tccccgtttg cccgcaagtc
     6961 caaaatggag cgatttccgc aaattagttt tgcgacatga aacagtcgat cctcttcctc
     7021 agcatgctcc tggtcatcgg ccaggtattc gcgcaagcgc ccccaaacag cggcgcccag
     7081 accggacgtt tgtacggcaa aatcgtggac tcggccggac acggcatcgg cagggcttcc
     7141 gtcctcatcc tcaaggcggg taccggcccg gccaccggca aaaaaaagca ggtgctcgtg
     7201 aaggggacga tcacccagaa caacggcgat ttctcggcgg aggacctgcc aatgggcagc
     7261 ccgctccagt taaatgtctc ggctgttgga tatacggcac tgcaacaggc agtcgtgctg
     7321 acgagacaag ctgcagacaa agatctcggc agcgtggtca tgctgcccga taccaaacag
     7381 ctggaacagg tggtcgtcac cgcctccaaa ccctccatga cgctcgatat ggataagaag
     7441 gtattcaacg tttcgaagga catcgtcagc gcgggcggca acggactgga cgtacttaag
     7501 aatgttccca gcgtcaacgt ggatatagac ggcaatatca ctctccgggg cggctcgccg
     7561 cagttattcg tcgacggcaa gcccactacg ctgacattgg acgaaatacc ttcggacgcc
     7621 atcgaaagcg tggaggtcat caccaatcct tccgccaaat atgacgcgtc gggtggtggc
     7681 gccggcatcc tgaacatcgt gttgaaaaag aaccgtaaga cggggtataa cggtagcgtc
     7741 cgggcgggtg ccgatgtcta cggtggtctc aatggcggat cggggctgaa tgtacggcag
     7801 ggaaagatca acttttcggc cgatctcaat ttccgtcagg gacgggacag gggcacaagt
     7861 tccatccagc gtctggatta cagcacgacg cccaataccg atctcaatca gaacgtgctg
     7921 gatacctcga agaacacgat gtatttcggc aggatcggcc tggattattt cctcaacaac
     7981 cggacaacgg tttccctcac agggttcgcc atgcatcacg gcggcatgca gacttccgat
     8041 atcaccatga acaccgatag cctctttacg gccggaaaga tcagccagaa tagccgcgaa
     8101 gagatcaacg gcgaccgcag cttcaacggc cggggagcga ccctcgggat caaacgcctg
     8161 tttgccaaag acaaggaaga atggacggcc gatgccagct atttttccgg caatgcttcg
     8221 aacaattcca tctattccac cgattattat caatatggca ccgcttcgac cctgacaggg
     8281 tcgacgctgc aaaagatcgc cggcggaggc aacgatcaca atctcatcct gcaaagcgat
     8341 ttctccgatc cgctgacgcc caagaccacg ctcgaggcag gcgttcgcgc agccctgcag
     8401 ggtcgtctga acatcaataa caattatacc tggaatgccg attcggccgc ctatgagcca
     8461 gtgccgtcgg ccgccagcaa ttacgaaagc cggagcaatg tctacgcggc atatgtcaca
     8521 ctgggcagca gcatcggcaa ttttacctac aaggccggat tacgcgccga aagctccaac
     8581 tatcatggca ctttgctgaa ttcagggcag tctttcgcca accaataccc gatcagtctt
     8641 ttcccgtcgc tgttcctggg acagaagata ggggatgacc aggaactcca gctcagctat
     8701 acgcgccgga tcaaccgtcc gaatttcttc cagctggtgc cctataccga ttcctccaat
     8761 aagctgaaca tcacccgcgg gaaccccgat ctcgttcccg agttcacgca atcgctcgaa
     8821 ctgtcctggc tgaagacgtt cagccgcaac agcacactgc tggcctccgt ctattataaa
     8881 cataccgatc acctgatcac gggctatatc gaggccgaca ccaacgcttc caccggcgcc
     8941 gtgagcttta tcaacacctt cgtgaatgcg gagtccagca attcgctcgg cgccgaactc
     9001 accgggcagt tcccgctgac gaggtggtgg gacgtatcgg cgaatgtcaa tctgtatcat
     9061 tcgcagatag atataggagg ctcggatgcc gtcgcacagc cggcgatgtg gagctggttc
     9121 ggaaagatga ataccaattt ccgtctcccg tcggggtttg ccgtccaggt gtcgggcatg
     9181 tatcagtcga agaccaatct gccagtgaac accagtgccg gtcagcccgg ccctccgaac
     9241 atgcaatcgc aaagcgcctc ccagggtttc atccggccgt attacgacat cgacctggcc
     9301 ctgagaaaga ccctgctgca gggaaagatg atcgcatcgc tgagctttaa cgacgtgttc
     9361 cggagccgca aacaggacca gtatacattc agcacctggt ttacgcagga ctatagccgt
     9421 ctgcgtaatc cgcagatgct ccggctcagc ctgacctgga atttcgggaa ggtggacgcc
     9481 agcctgttca aacggaagaa taacaatgta caggcggagg atcaataaat gtacccgcgc
     9541 agcggcgcag ctcgaataat cgactatatt tactactatg aagcttaggt ataaccggag
     9601 ggcgatccgc atcgtgacgc acgtggccgc gtgggcggtc gccctttcgc ttccctatct
     9661 gctggacagt catcacgggg tgcgtcatca taaggacgat atccacgaat gggatttctt
     9721 ttatctgaat ttcatcacca gcatcctctg gatcggcccc ttctatctga atgcctacct
     9781 cctgatgccc gcgttcttct atcgcaaacg ctatctcgcc tataccgccc tgctggcagc
     9841 gtcctttttc agcgtgtttc tgataaattt gcttttattc gtttatgcct tcgggttgcc
     9901 gcactataac ccgcagggcg ctatcggatt tctcctgccc acctttatcc tcttcatcgc
     9961 cgtcagcacg gcctatcgcg tcgtccggga taagacggag gcggacgagt tggcgcacca
    10021 gcggcacgag gagaacctca agaccgagct gtcctttctg cgtagtcaga tcaatccgca
    10081 ttttatcttc aacatcctga ataatctcgt cgcgctggag cagatgagat cgccggagct
    10141 ggggcccacc atcctgaaac tgtcggccct gatgcagtat atgctctacg agacggatga
    10201 ggaaaaggtg ccggtcaata aggagatcga atacctgcaa tgttatatcg atcttcagcg
    10261 tcagcgcttt ggcgcgaagg tgcccgtaag cctttcactc gatcaaacac cttcgatcta
    10321 cgagatagaa cctatgttgc tcatcccctt cgtggaaaat gcctttaaac acggcgttgg
    10381 aatgatcgaa cacccggcca tccatgtcag cctccatatc gaagacgggg tcctgctttt
    10441 ctcggtgcgc aaccggtata atccttcttc gacggagacc cgggacaagg gttccggcat
    10501 cggtctgggc aatgtcagcc ggcggctgcg cctgctatac ggaaatcagc aggtattgca
    10561 gatcagcagg gaggaggatt ggtttatcgt caaactggaa ttaaatttac actgatgcta
    10621 cgatgtatcg cagtcgacga cgaaccgctg gcgttgcacc tgctgacgga ttatatccgc
    10681 aaggtgccct tcctggatct tgttgccagc tgcgaagacg cctttcaggc ggcccgggtc
    10741 cttcaggaga accaggtcga cctgatcttc atggacatcc agatgcccgg tctcacgggt
    10801 ctgcaattca tccaaagtct ggccaaacgt ccgatgatca tcctggtcac ggcctacaag
    10861 aaatttgcca ccgaaggttt cgatctggat gtcgtcgact atcttgtcaa gccagtgggg
    10921 ctggacaggt ttatgaaagc ctgttacaag gcgcaggagc tgcaccaact gaggaccgct
    10981 ccacccgcaa cacccgctgg tgccaccggc gctgcggctc ccgactattt cttcgtcaat
    11041 tccgactata gcctggtaaa agtagtcttt gccgacatcg tctggatcga aggctccggc
    11101 gactatgtta agatccacct gcatagcgcg ccgcgcccgc tgctggtccg caccaacgcc
    11161 aagacgctgg aaggtgagct gcccgccgac aagttcctcc gtatacataa atcctacatc
    11221 atcgccatcg ccgccatcac ggcgatcaga aagaacagcg tcttcatcgg cgagctcgaa
    11281 ttgcctgtcg gcgacaccta tcgcgaaaca ttgcgaagga tcaccggacg ggaattatag
    11341 ttccgtaata ttctttatat tcaccaaatg actgctaatt caacctacct cgaatccaaa
    11401 agccattacc agatactcga cggacttcgt ggcgtcgcct cgatcatcgt agtagtcttc
    11461 catgtttttg aaacgttcaa tggcggaagc cgcttcgccc aattgatcaa ccatggctac
    11521 ctggcagtcg acttcttttt tgtgctttcg ggtttcgtcg tggcttatgc ctatgatgac
    11581 aggtggggaa agatgacgaa atgggatttt tacaaacgcc ggctggttcg tttgcagccc
    11641 atggtcgtca tggggtcgct gataggcgcc gctttattct atttccaggc cagccccgcc
    11701 ttcccgttgg tggccggtac acccgtatgg gtgatgctgt tggtgatgct ggtgggttgc
    11761 acgctgttac ccctgcccat ttcgatggac atccgcggct ggcaggaaat gcacccgctc
    11821 gatgggcccg cctggtcgct gttcttcgaa tacatcgcca attttcttta tgctgtggtc
    11881 atcaggcgac tgaataaact ggcgctctcg atcttcgtgg tattggcggc atgcctgctc
    11941 atccattata cggtgatggg accccagggc gacctcatcg gaggatgggc cctcaatggc
    12001 gaacagctca atatcggctt tacccgtgtc ctgtatccct tctttgcagg tgtactgctc
    12061 tgccggatcg gcaaattgcc tcaggtgaat ggcgctttct ggatctgcac gctgctcata
    12121 gcgatagtcc tcggcattcc ccgtatcgga ggcccggagc atctctggat gaatggcatc
    12181 tatgagtcgg cggtcgttat cttcgttttt ccttttatcg tttggcttgg cgcgggcggc
    12241 actatccgga acaaacggtc cgtccgcatc tgccggttcc tcggcgatat ttcctacccg
    12301 atgtatatca cccactatcc cctcatctat gtatttacgg cctgggtcag caagaacaag
    12361 atcccgatgt cgaaaggtct tcttcccggc gctcttttgg tggtcgccag cgtcgccatt
    12421 gcctatgcat gtctgaagct ctatgacgag ccggtacgcg aatggctgaa ggaaagagtg
    12481 ctgaaaaaga gcaaaccgga ggtacaggcc gcaatcaact aaaccctacc tgccgatctt
    12541 ccaacgtctt cttgcgcgcc ttgcgtagaa gatccagaga acgggaccga ttaaaggata
    12601 cacccatccg gccaaccctc ccacccgcgc cggcaatatc agcgctgtaa gcatcgaagt
    12661 agccccgatg atgatctgga tggcatgcga ccagatcctg gatctcgtat cgaatacctc
    12721 gaggtttgtc agctccaatc tgcccctgcg ccggaaagca tgcagatata gcagcaggaa
    12781 gacgacataa atgacgatat accccacact gtaaatgatc atcagctggg gtatctgctc
    12841 ctcccggata ggcctgacgg cgccaccggt attcccgccg agcagcagtg aaaaaagaaa
    12901 cttcagcgga tagacataga acaaaacgat aaataataac accgcattaa ggaacagcgt
    12961 cgttttgtcc tccatcccat accgccggaa aaagacatgc tgttcgttcc agatgatgaa
    13021 caggaaggta aagcagatcc cgaatgccac gaacccgcgg atcgacgtca gcagctcgtc
    13081 gaaggtcttc ggcacttcca gcgagacgat cagcagggtg atggcaaagg caaacacggc
    13141 atcgctgaat gtttcgatac gctgtatttc gtgaccgcgg tggcgaaagc cgccctcgcg
    13201 ccggggccgc cctgtgagta gtttcctgat cacaacagag tgtttaagac aatgctccga
    13261 atataggaaa attatcgcag cggagcgctg aaccactccc gcaccctgtt gcgttcgccc
    13321 catcccctga aaacaaactc attgctggcc ggatcataca cataatccgc cgcccattcc
    13381 cggtaccgct cgaccgtgat cccgatggca tcgaggatat acccgatctc gtcgtcggtc
    13441 atcgtaggat gcagcgatag acgcacccag cccggtttgc aagacagatc cccggatttc
    13501 agcgacgccc tcatcgcctg tgaccttgcc tcatcgatat gcaacaaaaa gtgtccgtag
    13561 ggacccgcac agctgcagcc acccctggtc tgaataccga atctgtcatt gagcatcttc
    13621 acgacgaggt tataatgccc gccggtcatc atgaatgata cgatgcccag acgtctgcga
    13681 taacccgcct ccaatatcgt gacaccgggc atccccggta gccggtcaaa gacgcattcg
    13741 agcaattcct cttcgcgccg gagcatttgc tcgacgccca tctcctcttt cagcctgata
    13801 caaagggccg cctttatcgc ctgcaagatg ggcggagtac ccccgtcctc acgctgttcg
    13861 atatccgaaa tatattcgcg tcctccccag gggttgctgt agaggatggt accacccccg
    13921 ggacgatcgg ggacgccacc tttatgtagc cgcccattaa agaccaggac tcccggcgta
    13981 ccggggccac ccaggaattt gtgacacgaa aaataaatgc cgtcgagatc gccgcccggc
    14041 tcaggcgggt gcatgtcgat cgatacatat ggcgccgaag aggcaaagtc gacgaagcaa
    14101 tatccgctat aggcatgtac cagcgccgct atggtatggt atggcgtctg aatgcccgtg
    14161 acgttcgaac aggcggtgac ggctgcgatc ttgaccctgc gatgtttgta ttttttcagc
    14221 aactccgtga aatattccag atcgacattc ccgtccgggg cagcccttat gatttccaca
    14281 gttgccccgg tctcgagcca gctgatgtgg ttgctgtgat gctccatgtg cgtaacaaag
    14341 acaactatct cttctttcga cgcccgaagg ccgagcattc gctgcaaccg gcaaacggcg
    14401 tccgtcattc cgctgccgca aaaaagtaac agatcgtccg tatccgcatg cacatgcgct
    14461 ttgatgatag ctttggcctc ctcgtaggct gccgtcatgg cacggccggt gacggtcgta
    14521 cccgtatgcg tattcgcaaa aaaaggcaat acgttgcact gaagcctttt ctcgatgggc
    14581 ccgtagccgc gtccgccggc cgtccaatcg gcatagatga cccggttgcg cccaaagggg
    14641 gactcgaaga actgccggcg gccgatgaca tggtcggcga aagacgagaa atagtcttcg
    14701 agcgggaggg ggcgcgggcc ggcgttattt tgtctttgcg gtagcatggg aattaaacag
    14761 atcatgtggc gcccgtccga atgcaccgtt aaaaaatgta gccagcacta cgacaaaaac
    14821 gaccagtccg gctacaacca accactgccc gcccgatgtc ttttgaatat cctgtttcga
    14881 acggacatcg caaacttccc ccggacaata ttgcgccctg tccttaaaga acaaaggata
    14941 gaatttacac cccagacaga taccgaagga tgtctcgaag aacaggaaaa taagacagac
    15001 cagacaggtg agacccgtaa tggggctata ggcgttgacc agtaccagga aaacgaacat
    15061 gacggccgaa agggccacgc ctatggccca ggcgaacttt ttttgcggag cgcctacgta
    15121 ttcgggcgtt tgccggcgga cgatccatcg cccgatgatc agcgtcgggg aataccgcgg
    15181 attcacaaag acgcggatga tgaaatcgag gaggaaaatg gtgacgacat atttcaccgg
    15241 aacgaaattg ccggtagcca ggatgatgtt cagggagatg aatgtcgcca ggaacaggat
    15301 gccggccgct gcccggatct cccgctcatt cagcacccta acggaatatc ccggtacatt
    15361 ttctccgaac tggatgatgt tgcccatggt gttatttttt ccgtaatact atctaatcga
    15421 ttggaatagt gcaagttgca ccaacagacg gccgatcgtg gtataccaac gccggtttca
    15481 ggtctacagc cgtccgggtt taagacggaa ggaaagattt accgatattt agtaccggga
    15541 aattgaccat gagatcgctt ttagacatac gtaggttcaa actgtacggc tgggtcggtc
    15601 tggggatagt ctgcatgtgt actatccagg aaatacacag cgaacataga gggttcttca
    15661 ttgcaatcct ggattgtctt tggctgacca tctggcttac gctggtgaac tatctcctct
    15721 atgaatatgc cgtcccgcgg ttacgttggc ggaaattcta tataggcatc ctgctgttca
    15781 ttttttatct gttcctgagt accgagggct ttcatctctg ggcgcaactg ggctatgcgc
    15841 tacacgtgaa tgtcgattac atcgagaccg cgggcaaacg ccatcatctt tccgaggaag
    15901 caagcttcag cctgccgtct tcgatcatgt tcggtatcgc gggcgttatc tttaataacg
    15961 tcaggctgcg gcaggctacc cagcagctgc gcatcgagaa acagcaggcc gagctcaatt
    16021 atctcaaatc gcaaaccaat ccgcatttcc tcttcaacac cctgaacaat atctatgcgc
    16081 tggcaaggga caggtccgac ctcgcgcccg aatcgatcat gcggctgtcg aagatcttac
    16141 ggtatatgct gtacgatgcc ggaggacaat atgtcgccat cgaacaggaa ctgaaggtca
    16201 tcggcgatta tatctcgctg gagcagctgc gttatgacga tacgctccgt gtaaatttca
    16261 attacgatgt ggaggacatg aaacagtcgc tgcccccgct attgctcgtc ccgatggtgg
    16321 agaacgcatt caaacacggc gcttccgaaa ccagaaaccg gccctttgtc gatatccatc
    16381 tttccattgc aagccgccaa ttggcttttt tggtgcgcaa ttccatcgaa ggacagcctg
    16441 atgagggaag tataaaggag aatatcggcc tctccaatct gcggcgtcag ctggaattgc
    16501 tgtatactga ctaccggttg aatgtggagc at
//
LOCUS       DASYSV010000621         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_766392, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000621 DASYSV010000000
VERSION     DASYSV010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766392"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VGQ51_19395"
     CDS             complement(<1..616)
                     /locus_tag="VGQ51_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV9038815.1"
                     /translation="MTIRSLAGVFLGLLLAVGVQAQSDSTYAERLGWPKGARVLIIHV
                     DDAGMSLESNEGAMAALTRGAATSVSVMMPCPWVPGFVKWLRSHPDIDAGLHLTLTSE
                     WADYRWGPLAGRGAVPGLVDSSGCLWASVGEAATHASPDEVAREINAQLERARAMGFH
                     PTHLDTHMGTVYARPDFLQQYIQMGIKNHIPVMLPGGADRLIREQ"
     gene            673..1119
                     /locus_tag="VGQ51_19400"
     CDS             673..1119
                     /locus_tag="VGQ51_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038816.1"
                     /translation="MQRSLFFQQCPIVSTIFPLPQAFNPHPNPEKAMSLAETYFIKTQ
                     LPPITTNDLLRLYERAIIEQGAGYTQIAENSIEFGETSSFTHRFGRKFAGFTSGRLVI
                     RETETTFEITLDAISPTWIPLFDVYFITLRDSIERELQSPASSINV"
     gene            1185..1673
                     /locus_tag="VGQ51_19405"
     CDS             1185..1673
                     /locus_tag="VGQ51_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038817.1"
                     /translation="MPTGAGAGLVLTSDASGNATWQTAQASTAGWSFGGNTVGVLKTL
                     GTVDNNDFPLITDNLERLRFTAGGNVLIGKTSQANSGYLLDVNGNIRANKIVVNTSGA
                     DFVFEPCYRLPSLQQDQTIRDYDQKFKQQEEQTKSMQERITRLEQLIDSSSALSTKHS
                     SK"
     gene            complement(1609..4395)
                     /locus_tag="VGQ51_19410"
     CDS             complement(1609..4395)
                     /locus_tag="VGQ51_19410"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEV9038818.1"
                     /translation="MKKYPTITFLLFLIAWRAYSQPDAELASRLSSARSRDDLSGWIY
                     AQIQWTAKAPASRSGQLADAVRGTWREPHSPEEQQAWLDLLTNEGYSMLLRGAIVPST
                     DAYTAAYDYARRHPAITDAGLVLETVLKPLGNNYTRLGDYEQALYIHQKAAALALALG
                     DREALAGVYSNLANTCGNMGRSLQARDYCFKGLAVVRPHSAMAGLLFSELADAYRGMG
                     VADSAKIFIRESISILRVAHDHPSAGYWLLTAYEEAGDLYGETSQALGYYQQALRLQG
                     RLALTAGELRQRDRAKLYWRLGSMLGVLGRKKESVGEKKEAMGRKEESVRWLDSCLAV
                     LLPGKDLTVLRPSDLYAENTLADALYALAGEATDADVAIRYYELSFAAGKKGRQELIT
                     TSSKERWVSDVRGRFERALGVAWDAWSRTRLVKYAYAMLGFMESSKAQLLLDEQLQHQ
                     QSGLDDSLGRRIRLLEKARVYYQKEMVGGGDSAALSGLRETDWELAKLRKMKPGLAVV
                     GPSSLLLPEGLVTRSYFAGDTVLYTVECGHSGIRYVDRLSLGAGWQDSVRAFMRYWFE
                     AGANHMIDDPGGYYRQAGGLYDRFFAAHPFDSGLAYILLPDGALSLLPVEALVVAPRG
                     GTSGAAGAPGAPESWPFVVRRAEISYGYSIQTLQRGGTGVSLQERRGAGDGGFSGFFL
                     AANQRDLPGLKATLEEKSGIEPFVREGHWYVDSSATVAAFKEALTGSSIVHISSHAFA
                     ARDSLDAPHIELYDAPFYLFEPFDLHPSLVVLSACRTGDGRFVSGEGVQSLARGFLAG
                     GAGAVVAGWWNVNDEVAASIMADFYARMASVSGGRGASVNAALALRQSKLDWLDDASR
                     EPLLHLPYYWAALSYTGNPEPFKDRASSGLVRRGGIADHERLLWGVIAGLVFILMSVW
                     WIRRRKSRSAARAA"
     gene            complement(4451..>4645)
                     /locus_tag="VGQ51_19415"
     CDS             complement(4451..>4645)
                     /locus_tag="VGQ51_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038819.1"
                     /translation="STDVHEAVSTTKSAVGYLGSVNAAPFIDLQLGAVRHGPVVGIRY
                     MRLLSGNITDRWFLYAGFKL"
BASE COUNT         1152 a         1573 c         1038 g          882 t
ORIGIN      
        1 tttgttcccg gatgagcctg tctgctccac cgggcagcat caccgggata tgatttttta
       61 tacccatctg tatatactgt tgcaggaaat ccggcctggc ataaactgta cccatatgcg
      121 tatccagatg ggtgggatga aaacccatcg cccgcgcacg ctccagctgc gcattgatct
      181 ccctggccac ttcatccggg ctggcatgtg tagccgcctc acctaccgat gcccaaagac
      241 aaccgctcga atctaccagt cccggtaccg cccctcgacc tgccagcggt ccccaccggt
      301 aatccgccca ttcggatgtt agcgtcagat gaagcccggc atcgatatcg ggatggctcc
      361 tgagccactt gacaaaaccc ggaacccagg gacagggcat catcacgcta accgacgtcg
      421 ccgccccgcg ggttagcgcc gccatagcgc cttcattgga ttcgagactc atcccggcgt
      481 catcgacgtg gatgatcaat acccgcgctc ctttcggcca tcccagccgt tcggcatacg
      541 tcgagtcgct ctgcgcttgc actcccacgg caagcagcag tcccagaaag acaccggcaa
      601 gcgatcttat tgtcataaat tcccttattt ctatgtcaat agcaatatca agataatcga
      661 taaacccgga aaatgcaacg atcacttttc ttccaacaat gcccaatcgt ttccacaatt
      721 ttccccctcc cccaagcgtt caatccccat ccaaaccctg aaaaagcaat gtcccttgcc
      781 gaaacctatt tcattaagac ccaactcccc ccaattacaa ccaatgatct tctacgttta
      841 tacgagaggg ctataatcga acaaggtgca ggctatactc aaatagcgga aaactccatt
      901 gaattcggcg aaacctcctc attcacccac cgttttggac gaaaatttgc cggcttcacc
      961 agcggccggt tggttatccg ggaaaccgaa accaccttcg agatcacatt agacgccata
     1021 tcccctacct ggatccccct cttcgatgtt tatttcataa ccctccgcga ttctattgaa
     1081 agagaactcc agtcgcccgc ctcctccata aatgtttaac cttataaaaa aaccataaag
     1141 agtaggaagg accgcctggc tccttcaaag ctaccagctt caatatgccg acaggcgccg
     1201 gtgccgggct ggtgctcacc agcgacgcaa gcgggaacgc aacctggcag accgctcagg
     1261 cctccaccgc cggctggtct ttcggaggca acaccgtagg cgtcctcaaa accctgggga
     1321 cagtcgataa caatgatttt cccctgataa ccgacaacct tgagcggttg aggttcaccg
     1381 caggcggcaa cgtcctcatc ggaaagacct ctcaggcgaa ttccggttat ctcctcgacg
     1441 taaacggtaa tatccgcgcg aataagatcg tagtgaatac ttccggcgcg gatttcgtat
     1501 tcgagccctg ctaccggctc ccctccttgc aacaggatca gaccatccgt gattacgatc
     1561 agaaattcaa acagcaggaa gaacaaacga aatcgatgca ggaacgcatc acgcggctcg
     1621 agcagctgat cgactcttcc tccgccttat ccaccaaaca ctcatcaaaa taaacaccaa
     1681 acccgcgatc actccccaca agagccgctc atgatcggcg ataccgccgc gccggaccag
     1741 tccgctggaa gccctatcct taaaaggctc cggattgccc gtgtaactga gcgcagccca
     1801 atagtaaggt aaatgaagca gcggctcgcg cgaagcatcg tccagccagt ccagcttgga
     1861 ttgacggaga gccaacgcgg cattgacaga cgctccacgt ccgcccgaaa ccgaagccat
     1921 ccgcgcatag aaatcagcca tgatggatgc ggcgacctca tcattcacat tccaccatcc
     1981 cgccacaacg gccccggcgc cgccggcaag aaaaccacgc gcgaggcttt gaactccttc
     2041 gcccgatacg aaccgtccat caccagtccg gcaggcgctc agcacaacca gcgagggatg
     2101 caggtcgaat ggctcgaaaa gataaaaagg cgcatcataa agctcgatat gcggagcatc
     2161 aaggctatcg cgcgccgcaa atgcatgcga gctgatatgc acgatcgacg agcctgtcag
     2221 ggcttcctta aaagcagcga cggtagcact gctgtccaca taccagtggc cctcacgaac
     2281 gaaaggttcg attcccgatt tctcctccaa cgtcgccttc aatcccggaa ggtcacgctg
     2341 atttgcagcc aggaagaacc ccgagaaacc tccgtccccg gcgccccgcc gctcctgcaa
     2401 cgacaccccc gttccgcccc gctgcaacgt ctgaatacta tacccatacg atatctccgc
     2461 ccgccgaaca acgaaaggcc acgactccgg agcacccggc gcccccgccg ccccactcgt
     2521 ccccccacgt ggcgccacga ccaacgcctc cacgggcaac aaactcagcg ccccatccgg
     2581 caacaggata tacgccaaac ccgaatcaaa aggatgcgcc gcaaaaaacc ggtcatacaa
     2641 cccgccggcc tgccgataat accctcccgg atcatcgatc atatgatttg cacccgcctc
     2701 gaaccaataa cgcataaatg cgcgcacact atcctgccag ccggcgccca aagaaagcct
     2761 gtcgacataa cggatccccg aatgaccgca ctctaccgta tacaacaccg tatcccccgc
     2821 aaaatagctt cgggtcacca acccctctgg cagcaaaaga ctagaaggac cgacaaccgc
     2881 caatccaggc ttcatcttcc gaagtttcgc caattcccaa tccgtctcac gaagacccga
     2941 caaggcagcg ctgtcgcctc ccccgaccat ttccttctga taatagaccc tcgctttttc
     3001 aagcaaccgg atcctgcgac ccagactatc gtccaaacct gactgctgat gctgtagttg
     3061 ctcatccaac agcagctgag ccttgctgct ttccataaag ccaagcatcg cataagcata
     3121 cttcaccagc cgggtccggc tccacgcatc ccaggccacg cccagcgctc tttcaaaacg
     3181 ccctctgaca tcggataccc atcgttcctt cgagctggta gtgatcagct cctgcctccc
     3241 cttcttcccg gccgcaaaag acaactcata gtaccgtatc gccacgtccg catcggtagc
     3301 ctcaccggcc aacgcataca atgcatcggc cagcgtattc tcggcataca ggtccgaggg
     3361 ccgcaagacg gtaaggtcct tccccggcaa cagcaccgcc agacacgaat ccaaccaccg
     3421 caccgattct tccttccggc ccatcgcctc ttttttctct cccaccgact ccttcttccg
     3481 gcccaaaacc ccaagcattg accccagtcg ccaatacaat tttgcccgat cccgctgccg
     3541 caattcgccc gccgtgagcg ccagccgccc ctgcagacgc aacgcctgct gataataccc
     3601 gagggcctga gaagtctcac cataaagatc acccgcctcc tcataggccg tcaacagcca
     3661 ataacccgcc gaaggatgat catgcgccac gcgcaaaatc gaaatacttt cgcgtataaa
     3721 tatcttggcg ctgtccgcaa cccccatccc gcgatatgcg tccgccagtt cggaaaaaag
     3781 caaacccgcc atggcagagt gaggcctcac cactgcaagc cccttaaaac aataatcccg
     3841 cgcctgcaaa gaccgtccca tattaccgca ggtattggcc agattgctat agacgccggc
     3901 cagcgcttcg cggtcaccca atgcaagggc gagtgccgcg gctttttgat ggatgtataa
     3961 ggcctgctca taatctccca atctcgtata attgttacct aatggcttga gcacggtctc
     4021 gaggacgagc cccgcatccg taattgccgg atgccgccgg gcatagtcat aggcggcagt
     4081 ataagcatcg gtcgagggca cgatcgcgcc gcgcaacagc atactatacc cttcattcgt
     4141 cagcagatcg agccacgcct gttgctcttc cgggctgtga ggttcgcgcc acgtcccgcg
     4201 cacagcatct gccagctggc cactccggct cgcgggcgcc ttcgccgtcc attgtatctg
     4261 agcatagatc cagccggaaa gatcgtcccg gcttcgggca gaggacagcc gtgaagcaag
     4321 ctccgcatcg ggctgtgaat aagcccgcca ggcaataaga aagagtagga aggttatcgt
     4381 cggatatttc ttcaccaaaa gtgcgcgaaa ggcgccatca gcaccggcat cgcgccctga
     4441 agataataaa tcaaagctta aatccggcgt acaggaacca acggtcggtt atattccccg
     4501 acaatagccg catataacgt atgccgacta ccggcccatg acgcaccgcg cccagttgca
     4561 gatctataaa aggtgcggca ttgacgcttc cgagatagcc gacagcggat ttggtggtgc
     4621 tcaccgcctc atgtacatcc gtcga
//
LOCUS       DASYSV010000622         8907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1659230, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000622 DASYSV010000000
VERSION     DASYSV010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659230"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VGQ51_19420"
     CDS             <1..224
                     /locus_tag="VGQ51_19420"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEV9038820.1"
                     /translation="DIVLERRKELAFEGDRKYDLNRLGLAIDRGQNKGGAAGDGYSIP
                     FPFEYRVAPIPQQEILRNEAIAKEQNPGY"
     gene            complement(285..725)
                     /locus_tag="VGQ51_19425"
     CDS             complement(285..725)
                     /locus_tag="VGQ51_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV9038821.1"
                     /translation="MIKLQVIGNLGKDCVTNTVNGKNVINFNVAHTEKFRDAQGNQKD
                     KTIWVECAYWTDRTGVAPYLKKGTQVYAEGTPEVRTYPKNDGTTGISLTLRVQTVQLL
                     GGRGDGGGGGGYSGGASEGYSAPSGQSRVSSASDITEPIDDLPF"
     gene            907..2007
                     /gene="mnmA"
                     /locus_tag="VGQ51_19430"
     CDS             907..2007
                     /gene="mnmA"
                     /locus_tag="VGQ51_19430"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV9038822.1"
                     /translation="MSRNGKVLVAMSGGIDSTVTALMLHDQGYEVVGITMKTWDYASA
                     GASKKETGCCNLDSFNDARMAAVQHGFAHFVLDIREEFGDFVIENFVDEYLAGRTPNP
                     CVLCNTHIKWRALLRRADALDCAFIATGHYGIVRQHTNGRYTIAKGVDETKDQSYVLW
                     GLQQDLLSRTLLPLGGYRKTAIRQMALDYGYPELAKKSESYEICFVPDNDYRGFLKRR
                     VDGLETRVAGGNFVDKHGNILGQHKGYPFYTIGQRKGLDITFGKPVYVTGIRPETNTV
                     VLGDEADLDRASMVVGKLNWMKYDGATPGMELTTKIRYKDKGTLSNIYPEPNGTVRVE
                     FFENAKGIAPGQSAVFFENDEVVGGGIIQRSV"
     gene            2047..2838
                     /locus_tag="VGQ51_19435"
     CDS             2047..2838
                     /locus_tag="VGQ51_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038823.1"
                     /translation="MKRRPGLILALTAVLVIVISCKKSIDYTKTSDYSGPLTDFFTPL
                     QIGKFAIYRLDSLNFYFYGQMDTITSYLAKDSVEDTIRDGQGRLAWLVTRYLSDTAGL
                     TGWSPSITYTVTPSTQRIELTEDNLRFIKLSWPISQGFSWLGNSYLPYAPYQDFFDFS
                     DDAHLSLGNWNYTYQQIGQHFSAGGQTYDSTLTILQQNDSINVPVLDPNSFASRTYWS
                     ETYAKHIGLVYRHTEMWEYQPPTPDGTQAGYKIGFKVTLTMLSHN"
     gene            complement(2967..4073)
                     /locus_tag="VGQ51_19440"
     CDS             complement(2967..4073)
                     /locus_tag="VGQ51_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fmu (Sun) domain-containing protein"
                     /protein_id="HEV9038824.1"
                     /translation="MRFDNQLRHALGILDTYTGDLPLHVWLKDFYRANKQMGARDRRL
                     LSTMVYGFYRLGHALRDQPAADRMLTGLYLCNDQPIDLLRHFRPDWDPTAPLAEKMAA
                     TGIEVADIFPWKEELSDGIDHEAFCLSFLRQPDLFVRIRPGHEEAVRRAVAGEFIPPS
                     TLRLPNGYKLEELLTPDNEVVIQDYSSQRIATFLQSDQPPTSFWDACAASGGKTILAH
                     DLFPDMEITVSDIRESILHNLRARFRTAGIEHYRSFLIDLSVDSPPERTASADLVLAD
                     VPCTGSGTWSRTPEQLYFFNPQKIGHYSALQKKIIGHLAPNATLVYCTCSVFKKENEE
                     IVAHLRKNLRLQRAGVIAGYHLRADTLFAARFTR"
     gene            complement(4107..5387)
                     /gene="odhB"
                     /locus_tag="VGQ51_19445"
     CDS             complement(4107..5387)
                     /gene="odhB"
                     /locus_tag="VGQ51_19445"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEV9038825.1"
                     /translation="MIDIKVPAVGESINEVTLVKWLKQEGQYVERDEVIAELESEKAT
                     FEVNAEKAGVLKTIGKEGDTLKIGDVVAQIDEAAARPEGQTAPTAGANGKAAATAATA
                     ATAKSEDNPHTHPSVVPAAQAPAATQAPATQTKATPVAAAIISDKKIDAGSITPTGTG
                     GKIVKNDVLGALANPGRPLGATLFSRKDRREKMSNLRKTVSRRLVEAKNTTAMLTTFN
                     EVDMSAVMGLRAKYKDKFKEKHGVGLGFMSFFTKATCYALQEWPAVNAYIDGEELVYH
                     DYCDISIAVSAPKGLVVPVIRNAESLSMAEIEKKVVELATKARDNKLSMEEMQGGTFT
                     ITNGGVFGSLMSTPIINIPQSAILGMHKIEDRAVVVGGQVVVRPMMYLAVSYDHRIID
                     GRESVSFLVRVKELLENPDQLLFGKDPLKALLEL"
     gene            complement(5411..8200)
                     /locus_tag="VGQ51_19450"
     CDS             complement(5411..8200)
                     /locus_tag="VGQ51_19450"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEV9038826.1"
                     /translation="MKDFSFITNSHPSYIEGLYQDYVRNPETVDPEFRKFFEGFDFAV
                     TQGRSGVLNGNGAAASNGKAVAAAPGVLTTTDGVDWKKELGAYRLILGYRNKGHLLAK
                     TNPIRPRKDRGANLDLGFFGFTEADMDKNFFAGNLIGLGTTTLRAILTHLQQCYAGNV
                     GIEFKYISDQKQIDWLTSEMEKKFLEPTPLAKQRRILEKLNQGVMFEKFLHTKYIGQK
                     RFSLEGGEASIAALDTIINTAAENTVQEVVIGMAHRGRLNVLANIMGKTYEQIFSEFE
                     GTAKLDQTMGSGDVKYHMGYGSDVQTDTGKTVHLKLMPNPSHLEAVDPLVVGFARCKA
                     DVLYQSDYDKILPVLIHGDASIAGQGIVYEVLQMSNLKGYDTGGTMHFVINNQIGFTT
                     DFDDARSSDYCTSLAAAIQAPVLHVNGDDAEAVVKCADIATRYRQEFNSDIFIDMVCY
                     RRHGHNEGDDPKFTQPHMYALIDKHPNPREVYTQHLLDNGEADAKDLAKEMEKKFWGD
                     LQERLDEVKQNPLPYTYQQPELWWRSLRKSNPADFDQSPVTAISEADFSKIFEAIMTW
                     PETFKPLRKVEKLLQDKVRLFKEENKVDWATGELLAYGSLLLEGKDIRMSGQDVQRGT
                     FSHRHAVLRDENTDEQYNRLSRLPDAKGQFRIYNSLLSEYGVLGFEYGYAMANPNALV
                     LWEAQFGDFSNGAQTVIDQFIAAAEQKWQRMNGVTLLLPHGYEGQGPEHSSARMERFL
                     QLCAEYNMVVTDITTSANLFHAFRRQLTWPFRKPLINFAPKANLRHPGTWSPMSDFIN
                     GGFREVIDNTFVEKAEKVKKVLFCSGKVYFDLAERQQKDDRKDVAIVRLEQLYPLPVQ
                     QLEGLYRKYSQAATWFWVQEEPLNMGAASFLQMNLKSINYGVISRQPSAATATGYSKV
                     HAQEQAEIIETAFSI"
     gene            8338..8601
                     /gene="rpsT"
                     /locus_tag="VGQ51_19455"
     CDS             8338..8601
                     /gene="rpsT"
                     /locus_tag="VGQ51_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV9038827.1"
                     /translation="MANHKATKKDQRQAEKRRDSNRYYGKTTRNAIRELRAVKGEKEA
                     GEKLPEVASMIDKLAKKGVIHKNKAANLKSKLARKINGLAAAK"
     gene            8712..>8907
                     /locus_tag="VGQ51_19460"
     CDS             8712..>8907
                     /locus_tag="VGQ51_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038828.1"
                     /translation="MRKYFSLLLLGLGLVAAAQTPAGAPRAPSTPFERSNGTQTATYA
                     ECIAFYQQLDRLSPALSIREM"
BASE COUNT         1860 a         2251 c         2689 g         2107 t
ORIGIN      
        1 atgacatcgt tttggaacgc aggaaggaat tggcattcga gggggatcgc aaatatgatc
       61 tgaacaggct gggtctggcg atcgaccggg ggcagaataa gggaggcgcg gccggcgacg
      121 gatacagtat accttttccg tttgaatatc gggtagcgcc gataccgcag caggagatat
      181 tgagaaatga ggcgatcgcg aaagagcaga atccgggcta ctgacgcggc gggtgcggcg
      241 cgcggcgcaa taaaaaaacc cggccgaggc cgggttttga ttttttagaa gggcaaatcg
      301 tcgatgggtt cggtaatgtc actggccgaa gaaacacgtg actggccgga aggggcgcta
      361 tatccctctg acgcgccgcc ggaatatccg ccgccgccgc cgccgtcgcc tctgccgccg
      421 agcaattgga cggtctgcac gcgaagggtc agcgagatac ccgttgtccc atcattcttc
      481 ggataggtcc tcacttcggg agtgccttcc gcatatacct gcgtaccttt cttcagatag
      541 ggtgctacac cggtacgatc cgtccagtaa gcgcattcta cccaaatcgt cttatctttc
      601 tgattgccct gggcatcgcg gaatttctcg gtgtgcgcca cgttgaaatt gatcacgttt
      661 tttccattca ctgtattggt gacacaatct ttgcccagat tgccgattac ctggagttta
      721 atcataattg tatttttatt gatgcagtaa agagggcaat ttaccacatt tataaagctg
      781 cgctggaaaa gttttcctcc gattacccac ctcttcgtaa atttgcggtt tggcgtggcg
      841 gcccttgcgg ggcataaagg aatggaagct cggaagagga gcggccgcgt ttgtaaacaa
      901 ttcattatga gccggaacgg aaaagtgctt gtggcgatga gcggcggtat cgacagtacc
      961 gtaacggcgc tgatgctgca tgaccagggg tacgaggtgg taggcatcac catgaagacc
     1021 tgggactatg ccagcgcggg cgcctcgaaa aaggagacgg gatgctgtaa cctggacagc
     1081 ttcaacgacg cgcggatggc ggccgtgcag cacggttttg cccatttcgt attggatatt
     1141 cgtgaggaat tcggggattt tgtcatcgag aatttcgtgg acgaatacct ggccggacgg
     1201 acgcccaatc cctgcgtgct ttgtaatacg catatcaaat ggcgggcctt gttgcgtcgg
     1261 gcggacgccc tggactgcgc gttcatcgcc acgggtcatt atggcattgt ccggcagcat
     1321 accaatggcc ggtataccat cgcaaaggga gtagatgaga cgaaggatca gagttatgtt
     1381 ctgtgggggt tgcagcagga cctgctgagc cgtacgttat tgcccctggg tggttatcgt
     1441 aagactgcga tccggcaaat ggcactggat tatgggtatc ccgagctggc gaagaaaagc
     1501 gaaagctatg agatctgctt cgttccggac aacgactatc gaggctttct taagcgacgt
     1561 gtcgacggtt tggaaacgcg ggtcgcgggc ggcaatttcg tcgacaaaca cgggaatatc
     1621 ctgggtcagc ataaaggcta tcctttctat accatcggac agcgcaaggg attggacatc
     1681 accttcggca agccggtcta tgtgacgggt atcagacctg agaccaatac ggtcgttctg
     1741 ggcgatgaag ccgatctgga ccgggcatcg atggttgtgg gtaagctgaa ttggatgaag
     1801 tatgacgggg cgacgccggg gatggagttg acgaccaaga taaggtataa ggacaaaggg
     1861 acgctgagca atatctatcc cgaacccaat ggcactgtgc gcgtggaatt cttcgaaaac
     1921 gcaaagggca tcgctccggg ccaaagcgcc gttttctttg aaaatgatga agttgtggga
     1981 ggcgggatca tccagcggtc cgtataatcc gcgacaataa atctacccgt ggaacgttac
     2041 aggtacatga aacgtcggcc cggtctcata ttggcgctaa ctgcggtatt ggtgatcgta
     2101 atcagttgta agaaatcaat cgactatacc aaaacctccg attattcggg tccgctgacc
     2161 gatttcttca cccctctgca gatcggaaaa ttcgctatct accggctgga ttcactgaac
     2221 ttttattttt acggacagat ggacaccatc acatcctatt tggccaagga ttctgtggaa
     2281 gacaccatcc gtgatggaca gggacgtctt gcatggctgg tgacccgtta cctgagcgat
     2341 acggccggtc tcaccggatg gtccccaagt atcacctata cggtgacgcc ttcgacccag
     2401 cggatcgagc tgacggaaga caatctccgt ttcatcaaac tgtcatggcc aatcagtcag
     2461 ggtttcagct ggctgggcaa ttcctatctg ccgtatgccc cttaccagga cttcttcgat
     2521 ttcagcgatg acgcgcattt gtcgctgggc aattggaatt atacctatca gcagatcggc
     2581 cagcattttt cggccggcgg ccagacctat gactccacac tgacgatact ccagcagaat
     2641 gactcgatca atgtgccggt actcgatcct aattcgtttg catcacggac atactggagc
     2701 gagacgtatg cgaaacatat cgggctggta taccggcata ccgagatgtg ggaatatcag
     2761 ccgcctacgc ccgacggcac gcaggccggg tataagatcg ggttcaaggt tacgctgacc
     2821 atgctgagtc ataattagcg cggggccagg ggcgcctggg gcgcggggcc gggggtgcga
     2881 ggttgggtgc ttggggcgtg aggccgggtg cttggggcat gaggttgggt gcctggggcg
     2941 tgaggttggg tgcgggccgc ggggggtcat cgggtgaagc gcgccgcgaa taaggtatcg
     3001 gcgcggagat gatagccggc gatgacgcca gcgcgctgta gtcgcaggtt tttcctgagg
     3061 tgggcgacga tctcttcatt ttccttttta aatacggagc aggtgcaata gacgagcgtc
     3121 gcattcgggg cgagatgtcc gataatcttt ttttgtagtg cgctatagtg tccgatcttt
     3181 tgcgggttga aaaaatacag ctgttcgggg gtccggctcc aggtgccgct gcccgtacag
     3241 ggcacgtcgg cgaggaccag atcggcggag gcggttcgtt caggcgggga gtcgacggac
     3301 aggtcgatga gaaagctgcg gtaatgttcg atccctgcgg tgcgaaaccg cgcccgcagg
     3361 ttgtgaagga tagattcgcg gatgtccgac accgttattt ccatgtccgg gaagaggtcg
     3421 tgtgcgagga tggtcttgcc gccgctggcc gcgcaggcat cccagaatga cgtgggtggc
     3481 tggtcggatt gcaggaatgt cgcgatccgc tgagaactgt agtcctggat gaccacctcg
     3541 ttgtcaggtg tcaacagttc ttcgagtttg tatccattcg gaagccgcag cgtcgacggc
     3601 ggtatgaatt cacctgcgac cgccctgcgg acggcctcct catgacccgg gcggatacga
     3661 acgaaaagat cgggctggcg gaggaaggat agacaaaagg cctcatggtc gatgccgtcg
     3721 gagagttctt ccttccaggg aaagatatcg gcgacctcga taccggtggc ggccatcttt
     3781 tcggcaagcg gcgcggtggg gtcccagtcg gggcggaaat ggcggagaag gtcgatgggc
     3841 tgatcgttgc agagatagag gccggtcagc atgcggtcgg cggcgggctg atcgcgcagt
     3901 gcatggccga gacgataaaa tccataaacc atcgtggaca gcagacggcg atcgcgcgcg
     3961 cccatttgtt tgttggcgcg gtagaaatcc ttcagccata catgcaaggg caggtcaccg
     4021 gtgtaggtgt cgaggatgcc gagtgcgtgg cgaagctggt tatcgaatct catgagcgca
     4081 tgctatgccg gccgatggcg tggggattac aattcgagca atgccttcag cggatccttg
     4141 ccgaagagca gctgatcggg gttctcgagc aattctttga cgcgtaccag gaagctgacg
     4201 gattcgcggc cgtcgatgat gcgatggtca tagctgacgg caaggtacat catcggccga
     4261 acgacgacct ggccgccgac gacgacggcg cgatcctcga tcttatgcat acccagtatc
     4321 gcggactggg ggatattgat gatgggcgtc gacatcaggc tgccgaaaac gccgccgttg
     4381 gtgatcgtga aagtgccgcc ctgcatctct tccatgctca gcttgttatc gcgggccttt
     4441 gtcgccagct cgacgacctt tttctcgatc tccgccatgg acaggctttc cgcgttgcgg
     4501 atgacaggga ctacgagacc tttcggcgcc gatacggcga tggagatatc gcagtagtca
     4561 tgatagacga gctcttcacc gtcgatatat gcgttgacgg ccggccattc ctgcaaggcg
     4621 tagcaggtag ccttggtgaa gaagctcatg aatccgagac caaccccatg tttctctttg
     4681 aatttatcct tgtatttggc gcgaaggccc atgacggcgc tcatgtccac ttcgttgaag
     4741 gtggtgagca tggccgtcgt attcttggct tcgacgagac gtcgtgagac cgtcttgcgg
     4801 aggttgctca ttttttcgcg acgatccttg cgcgagaaaa gggtggcgcc gagagggcgg
     4861 ccgggattgg ccaacgctcc gagcacgtcg ttcttaacga tcttgcctcc cgtgccggtc
     4921 ggcgtgatgg agccggcatc tattttttta tccgagatga tggcggcggc tacgggggta
     4981 gcctttgttt gggtggcggg ggcctgggtg gcggcggggg cctgggcggc ggggactacc
     5041 gagggatgtg tgtgcgggtt gtcttcggat ttggcggtgg cggcggtggc ggcggtggcg
     5101 gcagccttgc cattcgcgcc ggcggtggga gccgtttgtc cttcgggacg tgcagcagcc
     5161 tcatctatct gtgcgacgac atcgccaatt ttcagcgtat cgccttcctt accgatggtc
     5221 ttgagcacac cggctttttc cgcgttgacc tcgaaggtcg ctttttcact ctccagttct
     5281 gcaattactt catcgcgttc aacatactgt ccttcctgtt tgagccattt gaccaacgtt
     5341 acttcgttga tggactcacc tactgccgga actttaatat caatcatttt tcgataaaaa
     5401 ttttagtgtc ttatatactg aaggccgtct cgatgatctc cgcctgctcc tgcgcatgca
     5461 ccttggaata tccggtggct gtggcggcgc tgggttgccg gctgatgacg ccgtagttta
     5521 tcgatttcaa attcatttgc aggaacgagg cggcacccat attgagcggc tcttcctgca
     5581 cccagaacca tgtagctgcc tggctgtatt tccggtacag accttccagc tgctggacgg
     5641 gcagtggata cagctgttca agccgaacga tggcgacgtc tttgcggtcg tccttttgct
     5701 ggcgctcggc cagatcgaaa tagaccttcc cgctgcagaa caataccttc ttcacctttt
     5761 cagccttttc aacgaaagta ttgtcgatga cttcccggaa gccgccattg atgaaatcgc
     5821 tcatcggcga ccaggttccc gggtgtcgca ggttggcctt cggcgcaaaa ttgatcaacg
     5881 gtttgcggaa gggccaggtc agctgacgac ggaaggcatg gaagaggttg gcggacgtcg
     5941 tgatatcggt gaccaccata ttatattccg cgcagagttg aaggaaccgt tccatccggg
     6001 cgctgctatg ttcggggccc tgtccctcgt atccatgtgg cagcagcagg gttacgccgt
     6061 tcatccgttg ccatttctgt tcggcggcgg cgatgaattg atcgatgacc gtttgggcgc
     6121 cgttggaaaa atcgccgaac tgcgcctccc acagcacgag ggcattggga ttggccatcg
     6181 catagccata ttcgaaaccg agtacgccat actcgctgag cagcgaattg tatatcctga
     6241 actgcccttt ggcatcgggg agacggctta gccggttgta ttgttcgtcg gtattctcgt
     6301 cgcgaaggac ggcatggcga tgcgagaagg tcccgcgctg cacatcctgt ccgctcatcc
     6361 ggatgtcctt cccttccagc agcagactgc cgtaagccag gagctcgccg gtggcccagt
     6421 caaccttgtt ttcttcctta aagagcctga ccttgtcctg cagtagtttt tctactttgc
     6481 gcaggggctt gaatgtctcg ggccaggtca tgatggcttc gaatatcttt gaaaagtcgg
     6541 cttccgatat cgcggtgact ggagactggt cgaagtcggc gggattggat ttgcggagac
     6601 tgcgccacca caattccggc tgctggtagg tatagggcag cggattctgc ttgacctcgt
     6661 cgagcctttc ctgtaaatcg ccccagaact tcttttccat ttcttttgcg aggtccttcg
     6721 cgtcggcttc gccgttgtcg aggagatgct gagtatagac ctcgcgggga ttgggatgtt
     6781 tgtcgatcag ggcatacata tgcggctgcg tgaacttggg atcgtcgcct tcgttgtgac
     6841 catggcggcg atagcagacc atatcgataa agatatccga gttgaattcc tgccggtacc
     6901 gggtagcgat atcggcgcat ttgaccacgg cctcggcatc gtccccgttg acatgcaata
     6961 ccggcgcctg gatggcggcg gccagcgacg tacaatagtc cgagctgcgc gcgtcgtcga
     7021 agtcggtagt aaagccgatc tggttattga tgacaaagtg catcgtgcca cccgtatcat
     7081 agcctttgag gttgctcatc tggagcactt catagacgat accctggcct gcgatggatg
     7141 catcgccatg aataaggacg ggaaggatct tgtcgtaatc actttgatac aggacatccg
     7201 ccttacaacg ggcaaagccc accaccaggg gatcgacggc ctcaagatgt gaaggattcg
     7261 gcattaactt gagatgcacc gtcttcccgg tatcggtttg cacgtcgctg ccgtagccca
     7321 tatgatattt cacgtcgccg ctgcccatgg tctgatccag tttggccgtg ccttcgaatt
     7381 cgctgaatat ctgctcatac gtcttaccca tgatattggc gaggacgttg agacggccgc
     7441 gatgcgccat gccgatgacc acctcctgga cggtattctc ggctgccgta ttgataatgg
     7501 tgtccagcgc cgcgatcgag gcttccccgc cttcgagcga aaaacgtttt tgaccgatat
     7561 atttcgtgtg caggaatttc tcgaacatga cgccctggtt cagcttttcc aggatgcggc
     7621 gctgtttggc gagcggtgtg ggttcgagga atttcttttc catctcgctg gtcagccagt
     7681 cgatctgttt ctgatcgctg atatatttga attcgatgcc gacatttccg gcatagcatt
     7741 gctggagatg ggtgaggatg gcgcgaagcg tcgtcgttcc cagaccgata aggttgccgg
     7801 caaagaagtt cttgtccata tcggcctcgg tgaagccgaa aaagcccaga tcgagattgg
     7861 caccgcgatc tttccggggc cgtatggggt tggttttggc caggaggtgc cccttgtttc
     7921 gatagcccag gatgaggcgg taggccccga gctctttttt ccagtctact ccgtccgtcg
     7981 tggtcaggac gcccggagcg gcggcaacgg cttttccatt tgaagcggct gcaccgttac
     8041 cgttcaggac gcctgagcgg ccctgggtga cggcaaagtc aaacccttcg aaaaatttcc
     8101 ggaattccgg gtcgacggtc tcgggattcc tgacgtaatc ctgatagagt ccttcgatat
     8161 agcttggatg agaattagtt ataaaagaaa agtctttcat ggagatgtgc cgcggtgggc
     8221 atttttaggg ttacaaatat cgcttaaaaa atgggacgga cataggggtt tgtttattat
     8281 ttatttggaa tttccggtgg ttgggcgtac ctttgccgtc ctcaaaatgt cgttctaatg
     8341 gcaaatcaca aggcaacaaa aaaagatcaa aggcaagcgg aaaagcgcag ggatagcaac
     8401 cgttattatg gcaagaccac gcggaatgcc atccgggagc tgcgggcggt caagggagag
     8461 aaggaagccg gggagaaact gccggaggtc gcctccatga tcgataaact ggccaagaaa
     8521 ggggtcattc ataagaataa ggctgccaat ctgaagagca agctggcgcg gaagatcaac
     8581 gggttagctg ccgccaaata accggtagcg ccggccgggg ccgaccgatg gttttcggcg
     8641 tggggatcga cccttacaaa atagtttgat aatgaagcat cctgataagg gatgctttct
     8701 tattttaggg tatgcgaaaa tatttcagct tactgctttt ggggctaggg ctggttgcgg
     8761 cggctcaaac gcctgcgggt gcccctcggg ctccttccac acctttcgag cggtcaaacg
     8821 ggacccaaac ggcgacctat gccgaatgca ttgcctttta ccagcagctg gataggctct
     8881 cccctgccct ttcgatccgc gagatgg
//
LOCUS       DASYSV010000623         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_126057, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000623 DASYSV010000000
VERSION     DASYSV010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126057"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2106
                     /locus_tag="VGQ51_19465"
     CDS             <1..2106
                     /locus_tag="VGQ51_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEV9038829.1"
                     /translation="NPVNLVGYLSRDQYGDWPIAYGPDFTDQAPYIDKGDLYVKGDKK
                     YEIAGKKYGQDWGNTPSSHLFPRMWNPSNDRHEVDAYRRFSGMEPDDNPTMADNIKYF
                     IRYQTGWMYLRYFMWNFSGKQNDLEGFGNPRDGNAITGISFIDDYFLGDQSKMPDTIR
                     TQNKSYNRMYMLPLILGLIGLVFHYRRDRKGFIVIGLLFFFTGEAIVIYLNQAGFQPR
                     ERDYAFAGSFWTYSLWIGLGIIAVYDLFRRYAPKITPAAAAYTTAGICFLAVPVLMGN
                     QEWNDHDRHKKTLPRDLAKDYLESCPPNAMLFSFGDNDTYPLWYAQEVEGIRPDVRVI
                     VNTLLGTDWYMNSLRYKVNQNDPWDVIFTPDQIMGDRRAEVFFTDKIPGYDAGKYYDL
                     YSILKNVVASDEPRYTTPADNGQAINLIPTHKLLLPVDSAAAAATQAIHPGDSVLHEL
                     HMDIPANKNALFKNDLAMLAVIAGSKWHRPICFTSPQDLSGLGLDKYVRLRGMVYQLT
                     PTETGGADNDASYKIIMNDFAYGGAGKEGVYYDEENRRHLNSIKFAHAQVAMSLAAAG
                     KKDSARKILEHYDQNVLQSNMPYGMTSNQGNMHDYFSFDFLQACYMAGDSTLARKVSA
                     SLKKDLEQQMRYYKSLGDAGMSDEMLAINANMAMQNKGGNLSDQQAKYFVQDILSTYR
                     MLMQVTEWEKQNKTKTANP"
     gene            2140..>3390
                     /locus_tag="VGQ51_19470"
     CDS             2140..>3390
                     /locus_tag="VGQ51_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEV9038830.1"
                     /translation="MRFNRVNNIVGWIVGLVACTVFVMTMEATGSFWDCGEFISSCYK
                     IQIPHPPGAPLFVLMGRFFIILFGDNPHTAAHAVNFMSAIGSGLAILFLFWSVTHFAR
                     KLILKGDLMAEPTKRQTFMIMAAGVVGALAYCFTDSFWYSAVEGEVYASSAFFTALVF
                     WSILKWEQNADKPGADKWIIFIFYMMGLSIGVHLLNLLTIPAVVMVYYFRRFTPTTWG
                     TIFAFIVGCIITGIVQVVVIQYTIKGAGAFDIYFVNSLNLPFFTGFGFYFILLAALVA
                     WGLLFKRETVTPTKIRMWMMLFVLVILLPLVFSGTSSGGAGFVKLIIGLGLGWLAGYL
                     IKTDSLPKFKIALWCFGFMLLGYSTYFTTMIRSNADPGVDMYNVDNPVSLVGYLSRDQ
                     YGDWPILYGPDFTDRAPRVETGSLY"
BASE COUNT          763 a          979 c          863 g          785 t
ORIGIN      
        1 aaccccgtca atctcgtggg ttatctcagc cgcgaccaat atggcgactg gccgatcgcc
       61 tacggaccgg attttaccga tcaggctccc tatatcgaca aaggcgatct ctatgtcaaa
      121 ggcgataaga aatacgagat agccgggaaa aaatacggcc aggactgggg caatacgcct
      181 tcctcgcatt tgttcccccg catgtggaat ccgtccaacg accggcatga ggtagacgcc
      241 taccggaggt tctcgggtat ggagccggac gacaatccca cgatggcgga caatatcaag
      301 tatttcatcc gttatcagac gggctggatg tacctgcggt atttcatgtg gaatttctcg
      361 ggtaaacaaa acgatctcga aggcttcggg aacccccgcg acggcaacgc catcaccggc
      421 atctccttca tcgacgatta tttccttggc gatcaaagca agatgcccga taccatccgg
      481 acgcagaaca agtcgtataa caggatgtat atgctcccgc tgatcctcgg actgatcggg
      541 ctcgttttcc attaccggcg cgaccgcaaa ggatttatcg tcatcggtct gctgttcttc
      601 ttcaccggtg aagccatcgt catctatctc aaccaggccg gtttccaacc acgtgaaagg
      661 gattatgcgt ttgccggctc cttctggacc tactcgctct ggatcgggct gggaattatc
      721 gccgtatacg atctgttccg gcgatacgct cccaagatca cccccgccgc ggcagcctat
      781 accaccgcgg ggatttgttt cctcgccgta cccgtgctga tgggtaacca ggaatggaat
      841 gatcacgacc ggcataaaaa gacccttccc cgcgatctgg ccaaagacta tctcgaaagc
      901 tgtccgccca acgcgatgct cttctcgttt ggcgacaacg acacctatcc gctatggtat
      961 gcgcaggagg tcgaaggcat ccggcccgat gtgcgcgtca tcgtcaacac gctgctgggc
     1021 acggactggt atatgaactc gctgcggtat aaggtcaacc agaacgaccc ctgggacgtc
     1081 atctttaccc ccgaccagat catgggcgac cggcgcgccg aggtcttttt tacggacaag
     1141 atccccggtt acgatgcggg caaatactac gacctctatt ccatactgaa gaatgtggtc
     1201 gccagcgacg aaccgcgcta tactaccccc gcagacaatg gacaagcgat caacctgatc
     1261 cccacccata aattgctgtt gcccgtggac agcgcagcgg cggcggcgac acaggccatt
     1321 catcccggcg acagtgtcct tcatgaactg cacatggaca tcccggcgaa taaaaatgcc
     1381 ctgttcaaga acgatctggc catgctcgcc gtcatcgccg gcagcaaatg gcaccgtccc
     1441 atctgtttca cttctccaca ggatctttcc ggtctgggtc tggacaaata tgttcgcctg
     1501 cgcggcatgg tctatcaact gacgccgacg gaaaccgggg gtgccgacaa tgacgcctcc
     1561 tataagatca tcatgaatga tttcgcctat ggtggcgcag gtaaggaagg cgtttattat
     1621 gatgaagaga acagacggca tctcaacagc atcaaattcg ctcacgcaca ggtggccatg
     1681 agcctggcgg cagccggaaa aaaggattcc gcccgcaaga tattggaaca ctacgaccaa
     1741 aacgttctcc agtcgaatat gccctatggg atgacctcca accagggcaa tatgcacgat
     1801 tatttttcct tcgatttcct gcaggcctgt tatatggcgg gcgactcgac gctggccagg
     1861 aaggtgtccg catcgcttaa aaaggacctt gaacagcaga tgcgttatta caaatcgctg
     1921 ggcgatgcgg ggatgtcgga tgaaatgctg gccatcaatg ccaacatggc catgcagaac
     1981 aaaggcggca atctttccga ccagcaggcc aaatatttcg tacaggatat cctttccacc
     2041 taccggatgc tgatgcaggt gacggagtgg gaaaaacaaa acaagacaaa gaccgcaaac
     2101 ccttaatttc gtccctgaat taaaaaattt tcaataccaa tgcgtttcaa cagagtaaac
     2161 aatatcgtcg gctggatcgt gggactcgtc gcctgtacgg tttttgtaat gaccatggaa
     2221 gcgacgggca gtttctggga ctgcggcgag ttcatctcca gttgttacaa gatccagata
     2281 ccgcaccctc ccggcgcacc gttgttcgtc ctcatgggtc gttttttcat tatcctgttc
     2341 ggggataatc cgcatacagc cgcgcacgcc gtcaacttca tgagcgctat cgggagcggt
     2401 ctggccatcc tgttcctttt ctggagcgtc acgcatttcg cccggaaact gatcctcaaa
     2461 ggcgatctga tggccgaacc caccaaacgt cagaccttca tgatcatggc ggccggtgtc
     2521 gtcggggcgc tcgcctattg cttcacggac tctttctggt acagcgcggt ggagggggaa
     2581 gtctatgcat cctccgcctt ttttaccgct ctcgtattct ggtccatcct taaatgggaa
     2641 cagaatgccg ataaacccgg agccgataaa tggatcatct tcatcttcta tatgatgggg
     2701 ctctccatcg gcgtccacct gctcaacctg ctgaccattc ccgccgtcgt gatggtctat
     2761 tatttcagac gctttacccc caccacctgg ggcaccatct ttgccttcat cgtcggatgc
     2821 atcatcacgg gtatcgtaca ggtagtcgtc attcaatata ccatcaaggg cgccggcgcc
     2881 ttcgatatct atttcgtcaa tagcctgaat ttgcccttct ttacgggctt cgggttttac
     2941 ttcatcctgc ttgcggccct cgtggcctgg ggactgctct tcaaacgcga gacggtgact
     3001 cccacgaaga tccgcatgtg gatgatgctt ttcgtgctgg tcatcctcct tccgctggtc
     3061 ttcagcggga cgagcagcgg gggagccggt tttgtcaagc tgatcatcgg cctcggcctc
     3121 ggctggctgg cgggctatct gatcaagacg gacagcctcc cgaaattcaa gatagcgcta
     3181 tggtgttttg gcttcatgct cctgggctat tccacttatt tcaccaccat gatccgcagt
     3241 aacgccgatc cgggcgtgga tatgtacaac gtggataatc ccgtcagcct cgtgggctac
     3301 ctgagccgcg atcaatatgg cgactggccg atcctgtacg gtcccgattt tacggatcgc
     3361 gctccgcgcg tggaaaccgg cagtctctat
//
LOCUS       DASYSV010000624         5203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_1024174, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000624 DASYSV010000000
VERSION     DASYSV010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024174"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VGQ51_19475"
     CDS             complement(<1..1251)
                     /locus_tag="VGQ51_19475"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV9038831.1"
                     /translation="MDFSEKKRRRTNEEIFAHPRWHLFCLLIPVNTFLQKIGAWYEGG
                     STEFIVWAPLRKTVQLVLSDTSVHPMEKDEAGYWKTTLSVGPGTRYGFRLDDDKTYPD
                     PASLSQPEGVHALSAVIDRPASPWHDADWKGIPLSNKIIYELHTGVFSDSHDFEGVTR
                     RLDYLLELGVNTIELMPLGQFPGDRNWGYDGVYPFAIQHSYGGVPGFVRLVDAAHAKG
                     ISVIVDVVYNHLGPEGSYLREFGPYFTDRYKTPWGSALNFDEPWSDGVRNFFLQNARL
                     WLEDFHVDALRLDAVHAIFDFSAVHFMQQLTQLAGEISQRTGVKKELIAENDSNDPRY
                     INPPARGGYGLDGQWVDEFHHALRTLLTGDTSGYYEDFGRVSQLEKAFRQTYVHNGVY
                     SQHRHRTFGGHADNNPYDQFIVFAQ"
     gene            complement(1214..2182)
                     /locus_tag="VGQ51_19480"
     CDS             complement(1214..2182)
                     /locus_tag="VGQ51_19480"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV9038832.1"
                     /translation="MNEVKTGKRLAVGIFISLGLAILLVTIFTIGNQRKSFIRSIRLH
                     VVFDNVQGLQPGNNIWLSGMKVGTVKAVDFYGNSQVVVTLDVEQKAQPHIRKDSKAHI
                     STDGLVGNKIVVIDGGSNAAPIVSNNDSLPSEHIAGTQEMMSTFQASNSNLLEITGNL
                     KTISHRLTEGQGTLGRLINDPDIADHLRSSIDHLRNATAGSEKMIANLEAFSDRLRQP
                     DGLASKLATDTTVFTQIKGAIAQLNQAAAQVDTFSRTLRAAGEGLQNTNSPVGVVLHD
                     PDAAADLQRMLKNLRVSSKELSDDLEAVQHNFLLRGFFRKKAKTDQ"
     gene            complement(2179..2937)
                     /locus_tag="VGQ51_19485"
     CDS             complement(2179..2937)
                     /locus_tag="VGQ51_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV9038833.1"
                     /translation="MEPSNAVISLRNVHKSFGKQDVLKGVDLDVLKGENVVVLGRSGS
                     GKSVLIKIIAGLLKPDKGSVHVLGEEIDSLRTRELRALRSKLGFSFQNSALYDGMNVR
                     ANLEFPLVRNRRDLTRKEIDEAVTKVLDDVGLSQTVDQMPAQLSGGQKKRIGIARTLI
                     LKPEIMLYDEPTAGLDPVTSVDINDLIKEVQQLYNTSAVIITHDLTCARFTGDRVVML
                     SEGRFLRQGSFGEVFKSEDPKIRSFYNYNFIENT"
     gene            complement(2940..3689)
                     /locus_tag="VGQ51_19490"
     CDS             complement(2940..3689)
                     /locus_tag="VGQ51_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038834.1"
                     /translation="MRVFFLRIYDITQFIGRFFKEAFVPPYEYKEVLNQFFEIGIRSL
                     SIITLTGFITGVVFTKQSRSSLAGFGATAWLPSLIALAIIRALAPLVTALIVAGKVGS
                     NIGAELGSMKVTEQIDAMEVSSTNPFKFLVVSRILATTCIVPVLAIYMGFMGMMGAFL
                     NVYRNEQTGITLFFQQAFSRIDFLDIYVLVLKSAVYGFTIGVTGCFSGYQAEHGTVGV
                     GKAANTSVVVSMFLIFVEEMIIVSLVNNYLK"
     gene            3945..4700
                     /locus_tag="VGQ51_19495"
     CDS             3945..4700
                     /locus_tag="VGQ51_19495"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEV9038835.1"
                     /translation="MKAIIFHSTKTSFVTLILIICSISSLRAGTIYHNSSKAAAGRAA
                     RRAEMRMILATATLVYDEMDLEDSGLSRKAFESAWTGYYKLKKKGLLRNTHILSICDF
                     SQSSSNQRLYVIDTRNHRLLYRTYVAHGINSGSAYASSFSNRMESCKSSIGFYITSTT
                     YNGANGLSLRIDGLDKGFNDNARKRAIVIHGAGYVSRRILQKYGVMGTTFGCPAIPTE
                     MTSQIIPVVKNGSCFFIYYPSKKYLAQSPVLNS"
     gene            4765..5160
                     /locus_tag="VGQ51_19500"
     CDS             4765..5160
                     /locus_tag="VGQ51_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038836.1"
                     /translation="MKKTVVFIALLFGMAQLTTGQTLSYKDLLGKWEGTDEKSQTGAL
                     EFLDSSRMAMSMMGSAPRSLMYSIDFAKNPAAMDLYRDASKKGMALKCLIQLIDANTL
                     KWQVFPGGDRPDKFDEDSPGTLIVLKRKK"
BASE COUNT         1228 a         1361 c         1386 g         1228 t
ORIGIN      
        1 ttgcgcgaag acgatgaact gatcataagg gttgttgtcg gcatggccgc cgaaggtacg
       61 gtggcggtgc tgcgagtaca cgccgttgtg aacgtatgtc tgtcggaatg ccttttccag
      121 ctgggagacc cgtccgaaat cttcataata gccgctggta tcgccggtga gcagcgtgcg
      181 cagcgcatga tggaactcat cgacccattg tccgtcaaga ccatagccgc ctcttgccgg
      241 cggattgata tagcgcgggt cgttggaatc gttttcggcg atcagttcct ttttaacacc
      301 ggtccgctgg ctgatctcgc ccgccagctg cgtcagctgc tgcatgaaat ggaccgcgct
      361 gaaatcgaag atggcgtgta cggcatccag ccgcaatgca tcgacatgaa agtcttcgag
      421 ccacaaacgg gcattttgca gaaaaaaatt ccgcacaccg tcggaccagg gttcgtcgaa
      481 attgagcgca ctgccccagg gtgtcttata gcggtcggta aaataaggtc caaattcgcg
      541 aagatagctg ccctccggtc cgagatggtt gtatacgaca tcgacgatca cggagatgcc
      601 tttggcatgt gccgcgtcga ccagccggac aaagcccgga acgccgccat atgaatgctg
      661 gatggcgaaa ggatagacgc cgtcatatcc ccaattgcgg tcgccgggga attggcccag
      721 cggcataagc tcgatcgtat tcacgcccag ctccaggaga tagtccaatc tgcgggtgac
      781 accttcgaaa tcgtgactat ccgaaaaaac gccggtatgc agttcataga tgatcttatt
      841 ggagagtggg atccccttcc agtcggcgtc atgccatggg gaagccgggc gatcgatcac
      901 ggccgataaa gcatgtacac cctcgggctg gctcagcgaa gcggggtcgg gataggtttt
      961 atcgtcatcg agtctgaatc cataacgtgt gccgggaccg acggatagcg tcgtcttcca
     1021 ataacccgcc tcgtcctttt ccatcggatg gaccgaggta tcggaaagca ctaattgaac
     1081 cgtcttacgc aggggagccc aaacgatgaa ttcggtagat ccgccttcat accaggcgcc
     1141 tattttttgg aggaatgtat tcacggggat gaggagacaa aacagatgcc atcggggatg
     1201 agcaaatatt tcctcattgg tccgtcttcg ctttttttcg gaaaaatcca cgcagcagga
     1261 aattatgctg gacggcctcg agatcatcgc tcagctcttt gctgctgacc cgaagattct
     1321 tcaacattcg ttgcagatcg gccgcggcat ccggatcgtg aagaacgacg ccgacagggg
     1381 aattcgtatt ttgtaaaccc tctcccgcgg cgcgcaacgt acgactgaag gtgtcgacct
     1441 gtgcggcggc ctggttgagc tgggctatgg ctccttttat ttgtgtgaaa acagtggtgt
     1501 cggtagcgag cttgctggcc agtccgtcgg gttgtcgcag acggtcggag aaggcttcca
     1561 ggttggcgat cattttttca ctgcccgccg tcgcgttccg caggtggtcg atggaagacc
     1621 ggagatggtc ggcaatgtcc ggatcattga tcagccggcc gagcgtaccc tgtccctctg
     1681 taaggcgatg gcttatcgtc ttgagattgc cggtgatctc cagcagattg gaattgctgg
     1741 cctggaaggt gctcatcatc tcctgggtgc cggcgatatg ttcactggga agactgtcgt
     1801 tgttggaaac gatgggcgct gcgttgctgc ctccgtcgat gacgacgatc ttgttgccta
     1861 ccaatccgtc cgtgctgatg tgcgccttcg aatccttgcg gatatgcggt tgcgccttct
     1921 gctccacatc gagcgtgacg accacctgcg aattaccgta gaaatcgacc gccttgaccg
     1981 tcccgacctt cattcccgaa agccagatat tattgcccgg ctgcaaaccc tggacattgt
     2041 cgaatacaac atggagacgt atgcttcgga taaatgactt gcgctggttg ccgatggtaa
     2101 aaatggtgac cagtaagatg gccagtccca gcgaaatgaa aatgccgaca gccagtcgtt
     2161 tgcctgtttt gacctcgttc atgtgttttc gataaaatta tagttataaa aacttctgat
     2221 cttcgggtcc tcgcttttga atacttcccc gaaactgccc tgtcgcagga acctcccttc
     2281 cgacaacatg accaccctgt caccggtgaa cctggcacag gtgagatcat gcgtgatgat
     2341 gaccgcgctc gtattataca actgttgtac ctccttgatc aggtcgttga tgtccacact
     2401 ggtcacggga tcgagtcctg cggtcggttc gtcataaagc atgatctcgg gtttcaggat
     2461 cagggtacgc gcgatgccga tgcgtttttt ttgtccgccc gatagctggg caggcatctg
     2521 gtcgacggtt tgcgacagac cgacatcgtc gagcaccttg gtgacggcct catcgatctc
     2581 tttgcgggta aggtctctcc tgttgcggac gagagggaat tccagattgg cgcggacatt
     2641 catcccgtca tagagcgcgc tgttctggaa cgaaaagccg agcttcgaac gcaaggcccg
     2701 cagctcgcgg gttctcagac tatcgatctc ttctcccagg acatggacgc ttcctttatc
     2761 cggcttcaac aagccggcga tgatcttgat aagaacggat ttcccgctgc cgctgcgtcc
     2821 gagcacgacc acgttctcgc ctttgaggac atcgagatcc acgcctttca gcacgtcctg
     2881 tttcccaaag gacttatgga cattgcgaag agagatgacg gcattgctgg gctccatttt
     2941 tatttcaaat aattattgac taaagacacg atgatcatct cttcgacgaa gatcaggaac
     3001 atgctgacca caacggaagt attggcagcc tttccgaccc cgacggtgcc atgttccgcc
     3061 tggtaccctg aaaagcagcc cgtgacgccg atggtaaagc catagacggc ggacttgagc
     3121 accaggacat aaatgtcaag gaaatcgatc cgtgagaagg cctgctgaaa aaaaagcgtg
     3181 ataccggtct gttcattgcg ataaacattc aggaaggcgc ccatcatacc cataaaaccc
     3241 atatagatcg ccagcaccgg gacgatacag gtcgtagcga ggatgcggct gaccaccagg
     3301 aatttgaatg gattggtgga agagacctcc atggcgtcga tctgttcggt gaccttcatc
     3361 gaccccagct ccgcaccgat gttggaaccc actttacctg cgacgatcag cgcggtgacc
     3421 aatggcgcga gggcacgtat gatggcgagc gcaatcagcg agggtagcca tgctgtcgct
     3481 ccgaatcccg ccagcgatga ccgggattgc ttggtaaaga ccacacccgt gataaaacca
     3541 gtgagtgtga taatggacag cgaccggatg ccgatctcga agaattggtt gaggacctct
     3601 ttatattcat agggcggaac gaaggcttcc ttgaagaagc gaccgatgaa ttgagttata
     3661 tcatagatgc gtaaaaagaa aaccctcatt accttttcgg ggaaaatcta atgccaccag
     3721 gattcgatgt gtcttggggt tgaaattccc caggagacaa gttcgatgct cccttatggc
     3781 agcacccacg ggtttctggt caaaaacagg aggaaagctt ccaaaatccg gccgggtttc
     3841 ccggaatggg agaaaacgcc aaagattccc agatgcaaat atttgattat acaacaatta
     3901 cgtattggca cgtacttgga acgtattcct tcaaatccgt tatcatgaaa gcaataatct
     3961 ttcactccac caaaacttcc tttgtaacac ttatccttat tatctgcagt atctcgtccc
     4021 tgcgtgcagg aacgatctat cataactctt ccaaagccgc cgccggacgc gccgcccgac
     4081 gtgccgaaat gcggatgatc ctggcgaccg ccaccctggt ctatgatgaa atggatcttg
     4141 aagattccgg tctgagccgc aaagcattcg aatccgcgtg gaccggttat tacaaactca
     4201 agaagaaagg actgctgcgc aatacccaca tcctttccat ctgtgatttc agccagtcct
     4261 cgagcaatca gcggttgtat gtgatcgata cgcgcaacca ccgtttgttg tatcgcacct
     4321 atgtcgcaca tgggatcaat tccggctcag cgtacgcctc ctctttttcc aatcgcatgg
     4381 aatcctgcaa aagcagcatt ggtttttata tcacctctac cacctataat ggcgccaacg
     4441 gcttgtcgtt gcgcatcgac ggtctggata aaggtttcaa cgacaatgcc cgcaaacgcg
     4501 ccatcgtgat acacggcgca ggttatgtca gccggaggat cctgcagaag tacggtgtga
     4561 tgggcaccac gttcggatgt cccgccatcc ccacggaaat gaccagccag ataatcccgg
     4621 tggtcaagaa tggcagctgt ttcttcatct attatccttc taagaaatat ctggcccagt
     4681 cccctgtatt gaatagttaa gcaattgtgt tttcataggc tatgtatttt gccggaaatt
     4741 gtctattatt atcaaaaatt agctatgaag aagaccgtcg tttttatagc cctgctgttt
     4801 gggatggccc agctgaccac gggacagacc ctctcctata aggatctttt gggtaaatgg
     4861 gaaggcaccg acgaaaagag tcagaccggc gcgctcgagt tcctcgacag cagccgtatg
     4921 gccatgtcga tgatgggctc cgctccgagg agtcttatgt atagcatcga tttcgcgaag
     4981 aaccccgcgg ccatggatct ttaccgggac gcgagtaaaa aagggatggc gctcaaatgc
     5041 ctgatacagc tgattgacgc caatacgctc aaatggcagg tctttcccgg cggggatcgg
     5101 ccggacaagt tcgatgaaga ttcacccgga acgcttatcg tgctgaaacg aaagaagtga
     5161 agtctggttg gagcagatgg ccgatatttg tcggagcatt gta
//
LOCUS       DASYSV010000625        12687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_640103, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000625 DASYSV010000000
VERSION     DASYSV010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12687
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640103"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /gene="thrS"
                     /locus_tag="VGQ51_19505"
     CDS             <1..1674
                     /gene="thrS"
                     /locus_tag="VGQ51_19505"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV9038837.1"
                     /translation="GVKFWVGPPVDNGFYYDMDLGGRQVTEDDLRKLEVKMAELARGN
                     EIFRRKQISKADAISYFTEKGDEYKLDLLENLQDGNITFYTQGNFTDLCRGPHIPHTG
                     FIKAVRLTNIAGAYWKGDEKNKQLTRIYGVTFPSQKELDEYLAMLEEAKKRDHRKLGK
                     ELGIFTFDDQVGPGLPLWMPNGAVIIEQLETLAKETEGRAGYKRVVTPNIAKESLYIT
                     SGHVPYYQDSMYPPMELEGEKYYLKAMNCPHHHKIFAAEQRSYKDLPYRLAEYGTCYR
                     YEQSGELFGLMRVRCLHMNDAHIYCTKEQFYDEFKAVNEMYLKYFRIFGIDKYVMRFS
                     THDPARLGKKYIDEPGLWKETEDLVRDVLIRTGTPYVEVPDEAAFYGPKIDVQIWSAI
                     GREFTLATNQVDFAQGRRFDLSFTNRDNHPEVPLIIHRAPLGTHERFIGFLLEHYAGK
                     FPVWLAPLQVKILPITDKSLEYAQSLSILLKNADIRVEVDDRSEKIGKKIRDTELARV
                     PYMLVVGEKEMEGQKVAVRRQGLGDQGVKRIEEFIADIRKEITERTPNP"
     gene            1749..2345
                     /gene="infC"
                     /locus_tag="VGQ51_19510"
     CDS             1749..2345
                     /gene="infC"
                     /locus_tag="VGQ51_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV9038838.1"
                     /translation="MPFPPRPPRGNFNPRFRKEQQQEHRTNHMIRVPQVRLVGDNVEV
                     GVYSTQEAQKMAQDQQLDLVEISPNADPPVCKIIDYNKFLYEKKRKEKEMKAKSKASE
                     VKEIRFTPNTDDHDFDFKAKHAEKFLKEGNKVKAYVQFKGRAIQFKERGELLLLKFAE
                     RLNDSGVLEGMPKMEGKRMLAIWAPKPQKKKEKDKTAS"
     gene            2465..2662
                     /gene="rpmI"
                     /locus_tag="VGQ51_19515"
     CDS             2465..2662
                     /gene="rpmI"
                     /locus_tag="VGQ51_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV9038839.1"
                     /translation="MPKVKTNSSAKKRFKVTGTGKITHQKSFKRHILTKKSKKRKRNL
                     RQKGEVAASHMDFVKRLLRLK"
     gene            2704..3051
                     /gene="rplT"
                     /locus_tag="VGQ51_19520"
     CDS             2704..3051
                     /gene="rplT"
                     /locus_tag="VGQ51_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV9038840.1"
                     /translation="MPRSVNAVASRARRKRILKDAKGFYGKRKNVYTVAKNVLEKGLT
                     YRYVGRKLKKREYRTLWIARINAAVRAEGLTYSRFIHQLNAKGVALDRKVLADLAMNE
                     PETFKRLVAAVKG"
     gene            3167..4564
                     /locus_tag="VGQ51_19525"
     CDS             3167..4564
                     /locus_tag="VGQ51_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HEV9038841.1"
                     /translation="MNNTYTDLVTQTFNFPQEGFEVKDSYLQFNGLDIKALIDKYGTP
                     MKLTYLPKIGMQINKAKKMFASAIKKHRYEGKYFYCYCTKSSHFSFIVEEALKNDIHL
                     ETSYAYDIEIIKKLYQRKKITKDTFIICNGFKQKSYTSRIASLLNSGFKNVIPILDNK
                     EELQQYKRSVKVPFKLGIRVAAEEEPTFPFYTSRLGFRARDVLEFYVDKIEGNEDRFQ
                     LKMLHIFLNKGIKDDIYYWSELNKVINLYCQLKKICPELDSINIGGGFPIKHSLGFEY
                     DYQFMINEIVGNIKKACKRSNVPMPNIFTEFGSYTVGESMAHIYSVIGQKMQNDREIW
                     YMIDSSFITTLPDTWGIGERFLMLPINKWDVEYQRVVLGGITCDSHDYYDSEEHINEV
                     FLPKIVNGEPLYVGFFHTGAYQDQISGYGGIKHCLIPSPKHVIVGHDKNGKLVDWVYA
                     KEQTAQSMLKILGYM"
     gene            complement(4574..5860)
                     /locus_tag="VGQ51_19530"
     CDS             complement(4574..5860)
                     /locus_tag="VGQ51_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038842.1"
                     /translation="MIKPLGRVLRLWLFLSAGLFFFMKGYAQDTTVIHHIPMPADTVR
                     FEDSALRIKNLNPYFTLHVDSTLSYQFEINRDPAKYFFLLENAPVGLKIRENGMLFFR
                     ADKSFFLSGKLKYDHEYKVKLAVQNLNDPRDRIDTSFTIVFFTTEIIPSHVKPTVSNV
                     LYVDEGDTISFRVQCETGTYPIESINFYSSTPIKTNSLIRACNDDFTWSPPYDFVKET
                     DSAKQRLVLLYFVGTNKVFMRDTAVIKIYVKDALNYPLMVEQYNQVAKNMRTYILQLK
                     YVFVQLDKKVRKTKNTRTDFDMTTAGTALSGTVFSAQPSQSAKTTGAILPSVGLAVVP
                     VKETVAPTPTTEQNQAGLVRSSIKRLDYTLKDNALLGEKDPDIVKKIANLKNELKQTQ
                     VQLIDIPLEDVTGMTEEQLNAYFDNPKHNKKYRLKK"
     gene            complement(5970..6404)
                     /locus_tag="VGQ51_19535"
     CDS             complement(5970..6404)
                     /locus_tag="VGQ51_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV9038843.1"
                     /translation="MTLVKVNNNAHKTLSSFVDEFFQGFPAAGPGREESFGFPPVNIH
                     ETAEAYHLELSAPGRTKEDFKLSLDNGQLTIGFEKKDETKTEEYKTIRKEFSFRSFKR
                     SFNLDERIDTNGIQAKYENGVLKLYLPKKAEVKESAKQISIQ"
     gene            6627..7040
                     /locus_tag="VGQ51_19540"
     CDS             6627..7040
                     /locus_tag="VGQ51_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEV9038844.1"
                     /translation="MYPAEIVTPMKEELTDNGFHELLTSQEVDSQLQKSGTTLVMVNS
                     VCGCSAGSARPGVLMAVANSPKKPDYLTTSFAGFDMEAVSKIREHLLPYPPSSPSIAL
                     FKDGRLVHMIERHQIEGRPAQLIAHNLIAAFGEYC"
     gene            7334..8656
                     /locus_tag="VGQ51_19545"
     CDS             7334..8656
                     /locus_tag="VGQ51_19545"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEV9038845.1"
                     /translation="MYPNLYFAFRDLFGVSWPFLRFVNSFGFFVALSFIAAAVTLTRE
                     LIRKEREGLLQSREELLWIGKPVSPTELALNFILGFVVGFKLVGLFTADSALTANVQD
                     YLLSSQGNLPAGIVLGLLLAVWKWYEKNKQRLPQPQEKKVRIWPHDRVGDLTIYAFIF
                     GFIGAKIFNSLETWNDFVRDPVGSLLSFSGLTFYGGLICAALAIWNYARKHGIGFWHL
                     NDAAAPGLMLAYAVGRIGCQVAGDGDWGILNSAYITTPAGKVAPSNPQLFGQALQQYK
                     DVYLPQFGSLDKVPHLAAKAPSFLPDWLFAYPYPHNVISEGVRMIDCNGQYCSQLPIP
                     VFPTPFYETLVCLGLFFVLWALRRRLKIPGTLFGIYLVLNGVERFFVEKIRVNTKYDI
                     FGFHPTQAELISAGLVIAGVWIYIGLKRRGQPPVKAVSDPPDVNPIGK"
     gene            8918..11443
                     /locus_tag="VGQ51_19550"
     CDS             8918..11443
                     /locus_tag="VGQ51_19550"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV9038846.1"
                     /translation="MRSTHLFLTAVLFFVAIIPAVAQTGEKSAAAQNEGRSGASPSSA
                     RVSGSVGDAKGQPVESATISLLLAGDSSKVQQTATDKSGHFRLDHVPAGKYLIAVSSV
                     GFRKSYSVGFELGASANRTLPPIVLTAAAGDLQAFAVVAKKPFIEMKADKMVINVDAS
                     PSNAGSSAMDVLEKSPGVTLDKDDNISLKGKQGVTIMIDNKPTYLSATQLASYLRSLP
                     ASAIDQIELMTNPSARYDAAGNSGIINIRTKKNKMKGFNGNVNLTYNQGVYPKPSGSV
                     NLNYRNGKANFFLNGGYSNFEGFQQLDIGRKYLDQDGKTINSIFSQTTTMHFRNPGFN
                     LKFGMDYYLTNKTTVGFVVSGFSNKENMNSISTIQLQDPSKILDSVVYSPSTNNTNWK
                     NGSVNLNFRHTYDSTGRELTADMDYVRYSSVSDQYFDNLTYAPNSTLLNESILTGHLP
                     SAINIYTFKTDYTRPLAHGYKLETGIKLSYVNTDNTADYYNRVNKIDIVDTTKTNHFL
                     YRENINAAYATINKQFKKLAVQIGVRAENTNYSGHQLGNGQSTTINRDSSFSRSYVSL
                     FPTAYLTYTVNDKNQLTLNYGRRIDRPAYQDLNPFLFYLDQYTYQAGNPYLQPQFTHN
                     VELSHTYRQFLTTTLNYSYTQGFFSETFEQSGQATIVRNGNIGSRQNAGISVSAQLHP
                     AKWWTAILYSNLNYSQFNGILYGEPLNVSAATLLLNVNNQFMIHGGWSGEISGFYRTK
                     GVEGQIVIYPMGQASAAISKKILKDKASLKLAVRDIFYTNHPHGYINFQQTEATFQNR
                     RDSRQVALTFSWNFGKPLKGMSNGNRHNGAGDEESRVRTGGNN"
     gene            11509..12000
                     /locus_tag="VGQ51_19555"
     CDS             11509..12000
                     /locus_tag="VGQ51_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEV9038847.1"
                     /translation="MPSFDIVSKVDPQVLDNAVNVTKKEITNRFDFKDSPVKIDLNKK
                     DMKIDLEAESDMKLKQIVDVLISRSMKQGLDPLAFDMTKEPHQSGRVTRQEVPVRNGL
                     KQEDAKKVTKLIKESGLKVQTQIMDDLIRVTGKKIDDLQAVIKLCGGANLGMALQYVN
                     MRS"
     gene            12084..12338
                     /locus_tag="VGQ51_19560"
     CDS             12084..12338
                     /locus_tag="VGQ51_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV9038848.1"
                     /translation="MKKDIHPKGYRFVVFKDMSNGEAFLSKSSAQSRETIKWEDGNEY
                     PLIKLEISNTSHPFFTGKNVLVDTAGRIDKFKKRYEKKTK"
     gene            12407..>12687
                     /locus_tag="VGQ51_19565"
     CDS             12407..>12687
                     /locus_tag="VGQ51_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HEV9038849.1"
                     /translation="MHQLILEETSCTDDLLPFTATRSVLDIRMGILTFREKWERLLGS
                     GNFGLGNGPALPANILPTRALAARVREEMDRQPAIDRLITHGRLIRYPW"
BASE COUNT         3297 a         3071 c         3351 g         2968 t
ORIGIN      
        1 ggcgtcaagt tctgggtagg gcccccggta gacaacgggt tctattacga catggatctc
       61 ggcgggcggc aggtgacaga agatgacctc aggaaactgg aggttaagat ggccgaactg
      121 gcgcggggga atgagatctt ccgacggaaa cagatcagta aggctgatgc gatcagctat
      181 tttaccgaaa aaggagatga gtacaaactg gatttgctgg agaaccttca ggatgggaat
      241 atcacgttct atacgcaggg taatttcacc gatctctgcc gcggaccgca tatcccgcat
      301 acgggattca tcaaggcggt gagactgacg aatatcgcgg gggcctattg gaaaggcgac
      361 gagaagaaca aacagctgac ccggatctat ggggtcacgt tcccctcgca gaaagagctg
      421 gatgaatatc tggcgatgtt ggaagaggcc aagaaaaggg atcaccgcaa actgggcaag
      481 gagctgggca ttttcacttt cgatgaccag gtaggcccgg ggcttcctct ttggatgccg
      541 aatggcgctg tcattatcga gcagctggaa acgttggcca aggagacgga aggacgcgct
      601 ggttacaaac gggtcgtgac gccaaacata gccaaagaga gcctgtatat caccagcggc
      661 cacgtgccgt actaccagga cagcatgtat ccgcccatgg agctggaagg cgagaagtat
      721 tatctgaagg cgatgaactg tccgcatcat cataagatat ttgccgcgga gcagcgatcc
      781 tataaggacc ttccctacag gctggctgaa tatggcacct gttaccggta tgagcagagc
      841 ggggagctgt tcgggttgat gcgggtacga tgtctgcata tgaacgacgc gcatatctat
      901 tgcaccaaag agcagttcta tgacgagttt aaggccgtga atgagatgta tctgaaatac
      961 ttccggattt tcggtatcga taagtatgtg atgaggttca gcacccacga tcctgccaga
     1021 ttgggtaaga aatacattga cgaacccggg ttatggaagg aaacggaaga tttggtacgg
     1081 gatgtgctta tccggacagg gacgccgtat gtggaagtgc cggatgaggc ggctttttac
     1141 gggccgaaga tcgatgtgca gatctggagc gccatcggac gggaattcac gttggctacc
     1201 aatcaggtag actttgccca gggccggcgg ttcgatctat cctttacgaa tcgggataac
     1261 catcccgagg tgccgttgat cattcaccgg gcaccgctcg ggacgcacga acggtttatc
     1321 ggcttccttt tggagcacta tgcgggtaaa tttccggtat ggctggctcc gctgcaggta
     1381 aagatcctgc cgatcaccga taagtcgctc gaatatgcac aaagtctttc gattctgctg
     1441 aaaaatgcgg atattcgtgt tgaagtggat gaccgtagcg agaagatcgg aaagaagatc
     1501 cgggatacag agctggcgcg ggttccctat atgctggtgg tcggtgaaaa agagatggag
     1561 gggcagaaag ttgcggtgag gagacagggt ctcggcgacc agggcgtgaa gcggatcgag
     1621 gagttcattg cggacatcag aaaagagata acagaacgca cccccaatcc ctagggacgt
     1681 gcagacagac acaagaaaaa caattagcgg cttccgacag ggaggggagc tggatttaaa
     1741 aacagtaaat gccatttcca ccgagacccc cgagagggaa ttttaatcca cgttttagaa
     1801 aagagcaaca acaagaacat cgtaccaacc atatgatccg ggtgccgcag gtgcggctcg
     1861 tcggcgataa tgtagaggta ggcgtgtatt cgacgcagga ggcgcagaag atggcgcagg
     1921 atcaacagct tgacctggtg gagatatctc ccaacgccga tccccccgtt tgtaagatca
     1981 tcgattacaa caagttcctt tacgaaaaga agaggaagga aaaggaaatg aaggccaaaa
     2041 gcaaggcctc tgaggtaaag gaaatcaggt ttacgcccaa tacggacgat catgacttcg
     2101 atttcaaggc caagcatgcc gagaagttcc tgaaggaggg caataaggtc aaggcttatg
     2161 ttcagttcaa gggacgggcc atccagttca aggaaagggg cgagctgttg ttgctgaagt
     2221 ttgccgagcg gctgaatgat tcgggtgtgc tggaagggat gccgaagatg gaaggcaagc
     2281 ggatgttggc gatatgggcg ccgaaacccc agaaaaagaa ggaaaaagac aaaacagcgt
     2341 cataaatttg aaataaaccg ccacatggcg gtttttttta ttacctttgc atcccccaaa
     2401 tggccccata agtggtccgc cggtgcggat ccgccactcg ggtttaacac tcaatgcatt
     2461 cgagatgcct aaggtcaaga cgaattccag tgccaagaaa aggtttaagg taacaggcac
     2521 cgggaagatc acccaccaga agtccttcaa acgtcacatt cttactaaga aatccaagaa
     2581 acggaaacgc aacctccgtc aaaagggaga ggtcgccgct tctcacatgg attttgtaaa
     2641 acggctgctc cgtctcaaat agcgtcggaa atcaactcat ttaacacaca aacttattaa
     2701 gatatgccac gttcagtaaa tgccgtcgcc tcccgggcac gccgcaaacg catcctgaag
     2761 gacgcgaagg gattctatgg taaaagaaag aatgtctata ccgtcgccaa aaacgtcctg
     2821 gaaaagggcc tgacctatcg ttatgtcggc cgtaaattga agaaaagaga atacaggacc
     2881 ttgtggatcg cccgtatcaa tgcggcagtc cgcgctgaag gcctgaccta ttccagattc
     2941 atccaccaac tgaatgcaaa aggcgtcgcc ctggaccgga aagtgctcgc cgatctggcg
     3001 atgaacgagc cggagacctt caagcggctc gtcgctgcgg ttaaaggcta aaaattgtcc
     3061 tggcggacaa agctcctccg tcgctttttg aaaaaatgtt tatctttaga cgcccctttt
     3121 taaggggcgt ctaactttct gaatattgcc taacccggat cactcaatga acaatacgta
     3181 caccgacctg gtgacgcaaa ccttcaactt tccgcaagaa ggtttcgagg tgaaagacag
     3241 ctacctgcaa tttaacggtc tggatatcaa agccctgata gacaaatatg ggacgccgat
     3301 gaagctgacc tatctgccga agatcgggat gcagatcaac aaagcgaaga agatgtttgc
     3361 ctccgcgatc aagaagcatc gttacgaagg caaatacttt tattgttatt gtaccaagag
     3421 ctcgcatttt tcctttatcg tcgaggaggc cctgaagaac gatatccatc tcgagacctc
     3481 gtacgcctat gatatagaga tcatcaagaa actgtatcag cggaaaaaga tcactaagga
     3541 cacttttatc atctgcaacg gattcaagca gaagagctat acgagccgga tcgcgtcgtt
     3601 gctgaacagc gggttcaaaa acgtgattcc catactcgat aataaagaag agctgcagca
     3661 gtataaacgg tccgtgaagg tgcccttcaa actgggcatc cgggtggccg ccgaggaaga
     3721 gccgactttt ccgttttata cctcgcgtct tggcttccgc gcccgggacg tgctcgaatt
     3781 ctatgtcgac aagatcgaag gcaacgagga ccggttccag ctgaaaatgc ttcacatctt
     3841 tctgaacaag gggatcaaag atgacatata ctactggagc gagctgaata aggtcatcaa
     3901 cctgtattgc cagctgaaga agatctgccc ggagctggat tcgatcaata tcgggggcgg
     3961 gttcccgatc aaacactcgc tgggcttcga atacgactat caattcatga tcaatgagat
     4021 agtcgggaat atcaaaaagg cctgcaaacg cagcaacgtg cccatgccga atatcttcac
     4081 ggaattcggg agttatacgg tgggggagag catggcgcat atctacagcg tcatcgggca
     4141 gaagatgcag aacgaccggg agatctggta catgatcgac tcttccttta ttactacctt
     4201 accggatacc tggggcatcg gggaaaggtt tctgatgttg ccgatcaaca aatgggacgt
     4261 cgaataccag cgggtcgttt tgggaggtat tacctgtgat agtcatgatt attacgattc
     4321 cgaagagcat atcaatgagg tgtttcttcc gaagatcgtc aatggtgagc ctctgtatgt
     4381 gggctttttc catacgggcg cctatcagga tcagatcagc ggttacggag ggatcaagca
     4441 ttgtctgata ccctcgccca aacatgtgat cgtggggcat gacaagaacg ggaaactggt
     4501 cgactgggtc tatgccaagg aacagacggc gcagagcatg cttaagatac tcggatatat
     4561 gtagcccacc ctcttatttt ttcagcctgt atttcttgtt gtgtttcgga ttgtcgaagt
     4621 aggcgttcag ctgttcttcg gtcatccccg ttacgtcttc cagcgggata tcgatcagct
     4681 gcacctgcgt ctgttttagc tcgtttttga gattggcgat ctttttgacg atatccgggt
     4741 ctttttctcc cagaagggcg ttgtccttta atgtgtaatc cagacgtttg atagacgagc
     4801 ggacgagacc ggcctggttt tgttccgtag tcggggttgg cgccactgtt tccttgacgg
     4861 gtacgacggc gaggcctacg ctcggcagga tggccccggt agtcttggcg ctctggctgg
     4921 gctgtgcgga gaatacagtg ccgctgaggg cagtaccggc ggtagtcatg tcgaaatccg
     4981 tccgtgtatt cttggtcttt cttacctttt tatccagctg gacgaaaaca tatttcagct
     5041 gcaggatata ggtgcgcata ttcttggcca cctgattgta ttgttccacc atcagggggt
     5101 agttcaacgc atccttgaca tagatcttga taaccgcggt atcccgcatg aacaccttat
     5161 tggtaccgac aaaatacagc agcaccagcc gctgtttcgc cgaatcggtc tctttgacga
     5221 aatcataggg ggggctccag gtaaaatcgt cgttacaggc cctgatcagg gaattggtct
     5281 tgatcggggt gctcgaatag aaattgatgc tttcgatcgg gtaggtgccg gtttcgcact
     5341 ggacgcggaa actgatcgta tcgccttcgt ccacatacag gacattgctg acagtaggtt
     5401 tcacgtggga ggggatgatc tcggtggtga agaaaacgat cgtaaaactg gtgtcgatcc
     5461 ggtcccgggg gtcgttaagg ttctgtacgg ccagcttgac cttgtattca tggtcatact
     5521 tcagcttgcc ggaaaggaaa aaggatttat ccgcgcggaa gaagagcatt ccgttctccc
     5581 tgatcttcag cccgacgggg gcattctcga ggaggaaaaa gtatttggcg ggatcgcggt
     5641 tgatctcgaa ctggtaggaa agcgtggaat ccacgtgcag agtgaaataa gggttgaggt
     5701 tcttgatcct taacgcagaa tcttcgaagc gaacggtgtc tgcgggcata gggatatgat
     5761 ggatgaccgt cgtatcctgt gcataaccct tcatgaagaa aaacaaacct gccgaaagaa
     5821 ataaccaaag ccttaaaaca cgacccaaag gtttaatcat aattaattat tggatataaa
     5881 tcatttggag ccgtaaaatt aaaaaaaaaa agatgcccgg ccaaaggccg ggcaacatgg
     5941 gaacgagaat tattgggtcg atagccgaat tattggatcg atatctgttt ggcgctttcc
     6001 ttcacttccg cttttttggg cagataaagt tttagcacac cgttctcata tttggcctgg
     6061 atgccgttgg tatcgatacg ttcgtcgaga ttgaagctcc ttttgaagct ccggaaagaa
     6121 aactctttac ggatggtctt gtattcttcg gtcttcgttt cgtctttctt ctcaaagccg
     6181 atggtcagct gaccattgtc gagagaaagc ttgaaatctt ctttggtgcg gccgggagcg
     6241 ctgagctcga gatgataggc ttcagccgtc tcatggatgt tcacaggggg aaagccgaag
     6301 ctttcttcgc ggcccggacc ggcggcgggg aatccctgaa agaattcgtc tacaaagctc
     6361 gacagggtct tgtgtgcgtt gttgtttact tttacgagtg tcataaaatg gtatttgagt
     6421 gtttttaagt cgtttgacat gtggggagga acaaaccatg taccatccgg attttgaccg
     6481 ctttttcgcg aaaatctgac cgatatggcg acgacttaat gacataattt cattaaaaga
     6541 cggattttga tgacattttt gcataagcga ccgaaccaac tttttggcta attttgtgtt
     6601 ttaatactac ctaaacaatt tttaaaatgt atccagcaga gatagtaaca ccgatgaagg
     6661 aggagctgac ggacaatggg ttccacgaat tgctcacctc ccaggaagtc gattcgcaat
     6721 tgcagaaatc agggacgacg cttgttatgg tcaattcggt atgcggttgt tccgcgggtt
     6781 ccgcgcgtcc gggagttttg atggcggtgg ccaatagccc gaaaaagccc gattatttga
     6841 ccacgagttt cgcgggtttc gatatggagg cagtcagcaa gatccgggag catttgctgc
     6901 cttacccgcc ttcttcgccc tcgattgccc tgttcaagga tggccggttg gttcatatga
     6961 tcgagcgtca tcagatagag ggacgtccgg cgcagctgat cgctcataac ctgatcgccg
     7021 cgtttggtga atattgctaa agttcttcac gtaaaacaaa aagccccgcc gtcaggcggg
     7081 gcttttcttt tgtatccaca actgtggata agctatggaa tgcaggaata gtgaggattt
     7141 gcgaagaaaa atgttccccg tggaacactt atgtggaacg gcaatcaaca aatcgtagta
     7201 tgtgtggaaa attatttttg gtgagggtcc gcgtaagttg ctcaggttcc ggtggttcgg
     7261 gcccaaaaaa tatttcatct ccattgatgc cacaaaaacg taggtttgtg gcaaattgtg
     7321 ttagccctca tctatgtatc caaaccttta tttcgccttt agagatcttt ttggcgtttc
     7381 ctggccgttt ctgcgttttg tcaattcctt tggcttcttc gtagcccttt cctttattgc
     7441 ggctgcggta acgctgaccc gcgagctgat caggaaggag agggaagggc ttttgcaaag
     7501 cagggaagaa ttgctttgga tcgggaaacc ggtgagccct acggaattgg ccctcaactt
     7561 catcctggga ttcgtagtgg ggtttaagct ggtgggtctt ttcaccgccg acagcgccct
     7621 gacggccaat gtgcaggact atcttttgtc ctcgcaggga aatctgcctg cgggtatcgt
     7681 tctggggcta ttgctggcgg tatggaaatg gtatgagaag aacaaacagc ggcttcctca
     7741 gccccaggag aaaaaggtgc gtatctggcc gcacgaccgg gtcggggacc tgaccatcta
     7801 tgcctttatc tttggtttta ttggcgccaa gatattcaac agcctggaaa cctggaacga
     7861 tttcgtccgg gatcctgtag gctcgctact ctctttcagc ggattgacgt tctatggcgg
     7921 cctcatctgc gccgcgctgg ccatctggaa ttatgcacgg aaacatggca taggtttctg
     7981 gcacctgaat gatgccgcgg ctcccgggtt gatgctggcc tatgccgtcg gtcggatcgg
     8041 ttgccaggta gcgggggatg gcgactgggg aatactgaac agcgcttata tcacgacgcc
     8101 ggcggggaaa gtcgcgccat cgaacccgca actattcggt caggccttac aacaatataa
     8161 agatgtatat cttccgcagt ttggaagcct ggacaaggtg ccgcacctgg ccgcgaaagc
     8221 tccatccttt ttgcccgatt ggctgttcgc ctatccctat ccgcacaatg tgatcagcga
     8281 aggggtccgg atgatagatt gcaacgggca atactgcagt cagctgccga tccccgtttt
     8341 tcccacaccc ttttatgaga ccctcgtctg cctgggactg ttcttcgtgc tctgggcgct
     8401 gaggcgcagg ttgaagatac ctggtacact tttcggcatt tacttggtgc tcaacggggt
     8461 agagcgattt tttgtcgaga agatccgcgt caataccaaa tacgatatct tcggattcca
     8521 cccgacccag gccgaactga tctccgccgg attggtgatc gcgggggtat ggatctatat
     8581 cggacttaaa cgacgcggcc agccaccggt taaagccgtt tcggaccctc ccgacgtgaa
     8641 tccgatcgga aaatagcctt tttggagcag cggctgtccc cctcgccatc cttccgggaa
     8701 tctttccatt tgtcccaaat gccgacacat ttgtaacgcg aaatagtcat tccgcgtcac
     8761 aggttgcatc cttgcaaacc cggatttttt gattttccag agatgtgacc aactatcagt
     8821 aaaatcggcc atccaacatt aaaccatgtt ctatgtaatg ccaagtacca atctttgtat
     8881 tgtattaatc ataactaaga actagaaaaa actttatatg agaagcaccc acctttttct
     8941 aactgctgtc ctgttcttcg ttgccatcat tcctgcggtg gcccaaaccg gggagaaatc
     9001 tgcagccgct caaaatgaag gcagatccgg agcctctcca tcttcggcca gggtctccgg
     9061 cagcgtcgga gacgcaaaag gacagcccgt cgaatcggcg accattagtt tactgcttgc
     9121 cggagactct tccaaggtac aacagacggc aaccgacaaa agcggtcatt tccggttgga
     9181 ccatgtcccg gctggcaaat acctgatagc tgtatcatcg gtgggcttcc ggaagtccta
     9241 ttccgtggga ttcgagctgg gagcctccgc caaccggacc ctgcctccga tagtgttgac
     9301 ggctgccgcc ggcgatctgc aggcctttgc cgtcgtggct aaaaagccct tcatcgaaat
     9361 gaaggccgac aagatggtga tcaacgtgga tgcttctccg tccaacgcgg gctcctcggc
     9421 gatggatgtg ctggaaaaat ctcctggcgt cacattggat aaagacgaca atatcagtct
     9481 taaaggtaag cagggtgtaa cgatcatgat agataacaag cccacttatc tttccgcaac
     9541 gcagctggcc agctatctgc gcagccttcc ggcttccgcc atcgaccaga tcgaactgat
     9601 gaccaatccg tccgccagat acgatgccgc cggcaattcc gggatcatca atatccggac
     9661 gaaaaagaat aagatgaagg gattcaacgg gaacgtcaat ctcacatata accagggggt
     9721 ttatcccaaa cccagcggaa gcgtcaatct caactaccgc aacgggaaag ccaatttctt
     9781 cctgaatggg ggatattcca atttcgaagg attccagcag ctggacatcg gccgcaagta
     9841 cctggaccag gatggcaaaa cgatcaactc cattttttcg caaacaacga ctatgcattt
     9901 ccgtaacccc ggcttcaatc tgaaattcgg gatggattat tacctgacga acaagacgac
     9961 ggtcggtttc gtggtcagcg gtttcagcaa taaagaaaat atgaactcca tcagcacgat
    10021 ccagctgcag gacccgagta agatactgga ttccgtggtc tattccccga gcaccaacaa
    10081 tacaaactgg aagaacggat ccgtcaatct gaatttccgc catacctatg attccaccgg
    10141 gcgcgaactc accgcggata tggactatgt gcgttatagc agcgtcagcg atcagtattt
    10201 cgacaacctg acctatgcac cgaacagcac gctgctcaat gagagcatcc tcaccggtca
    10261 tctgcccagc gcgatcaata tatacacttt caaaacggat tatacccgtc cgctggcgca
    10321 cggttacaag ttggagaccg gcatcaagct cagttatgtc aataccgaca ataccgccga
    10381 ttattataat cgggtgaaca aaatcgatat tgtggatacg accaagacca atcatttcct
    10441 gtaccgggaa aatatcaatg ccgcctatgc gacgatcaac aaacaattta aaaagctggc
    10501 cgttcagatt ggtgtccgcg ccgagaacac caattatagc gggcatcaat tgggcaacgg
    10561 gcagtccacg accatcaacc gcgactccag cttttcgcgc agctatgtaa gcctgttccc
    10621 caccgcctat ctgacctata cggtcaacga caaaaaccag ctgacgctta actatggccg
    10681 tcgtatcgac cgacctgcct accaggacct gaaccccttc ctgttctatc tcgaccagta
    10741 tacttatcag gccggcaatc cttatctgca gccacagttc acgcacaatg tcgagctgtc
    10801 gcatacctac cggcaattcc ttaccacgac cctcaactac agctataccc agggtttctt
    10861 ctcggagacc ttcgaacaat ccgggcaggc aacgatcgtc cgcaatggca atatcggcag
    10921 ccgtcagaat gccggtatct cggtcagcgc ccagctccat ccggccaaat ggtggaccgc
    10981 gatcttgtat agtaacttga attacagcca gttcaatggc atactttatg gcgaaccgct
    11041 gaatgtctcg gctgccaccc tgcttttgaa tgtgaataac cagttcatga tccacggggg
    11101 atggagtggc gaaatatccg gattctaccg gaccaaaggc gtagaaggtc agatcgtcat
    11161 ttacccaatg ggacaggctt cggcagccat cagcaagaag atcctgaagg acaaggcctc
    11221 gctgaaactg gccgtcaggg atatctttta taccaaccac ccgcatggct atatcaattt
    11281 ccagcaaacg gaggctacct tccagaaccg ccgggatagc cggcaggtgg cccttacttt
    11341 cagctggaat ttcggtaaac cgttgaaagg catgtccaat ggcaaccggc ataacggcgc
    11401 aggcgatgag gaaagccggg tcaggacggg cggaaacaat taggaggcgg aagcccgagt
    11461 atcgtacgga tgcagtagat ttgagtcgaa aacaaaatcg cccatcttat gccatcgttc
    11521 gatatcgtca gcaaagtgga tccgcaggtg ctcgataacg cggtaaacgt caccaaaaag
    11581 gagatcacga acaggttcga tttcaaggat tccccggtga agatcgattt gaataaaaaa
    11641 gatatgaaga tcgatctgga ggcggaaagc gatatgaagc tgaaacagat tgtcgacgtg
    11701 ctcatcagcc ggagcatgaa acagggtttg gacccgctgg ctttcgatat gacgaaggag
    11761 ccgcatcaaa gtggcagggt cacccggcag gaggtgccgg tacgcaacgg tctcaaacag
    11821 gaagacgcca aaaaggtgac caagctgatc aaggaatccg gtttgaaggt acagacgcag
    11881 atcatggacg acctgatacg ggtcacggga aagaagatag atgatctgca ggccgtcatc
    11941 aaactgtgcg gcggcgccaa tctggggatg gcgctgcaat atgtgaatat gcggagttag
    12001 atttttttgc ccgacccgca taatttacct acttttgcaa cccggtacgg caaaatccgt
    12061 actagcttaa aaaagaataa gttatgaaaa aagatatcca cccgaagggt taccggttcg
    12121 ttgtttttaa agacatgagt aatggagagg ctttcctgag caaatcatcc gctcaatcca
    12181 gggaaaccat caaatgggaa gacggcaacg aataccctct cataaaactg gaaatttcga
    12241 atacctcgca tcccttcttc accggtaaga acgtcctggt cgacaccgcg ggccggatcg
    12301 acaagttcaa gaaacgttac gagaagaaaa cgaaataatt tacgcattcc gcgcgtgaac
    12361 gatcccctcc tgataggagg ggattttttt atagatttgc cgggccatgc atcaactcat
    12421 actcgaagag acctcctgca ccgatgacct gcttcccttt actgctacac ggtccgtgct
    12481 cgatatccgt atgggtatcc tgacgttccg ggagaaatgg gagcgtttgc tgggcagcgg
    12541 taatttcgga ttgggcaacg ggccggcgtt gccagctaat attcttccta cacgtgcgct
    12601 cgcggcaagg gtccgtgaag aaatggatcg acagcccgcg atcgaccggc tgatcacaca
    12661 tggccgtctg atacgctatc cctggca
//
LOCUS       DASYSV010000626         4559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4548 k141_511931, whole genome
            shotgun sequence.
ACCESSION   DASYSV010000626 DASYSV010000000
VERSION     DASYSV010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958632
            Sequence Read Archive: SRR11742949
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 16:19:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,918
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 21
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511931"
                     /isolate="SMAG_U4548"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.1681 N 96.4109 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VGQ51_19570"
     CDS             complement(<1..328)
                     /locus_tag="VGQ51_19570"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV9038850.1"
                     /translation="MLLQDFIEGAVPVKTFQKGDLIYRQDAHARYFYEVKDGEIAIVN
                     SNSEGKEFIQGLYKAGHCFGVAALIDDVPYISAAIAQTPCEISIITRDHFFRLLKGNY
                     DFHLRIT"
     gene            354..824
                     /locus_tag="VGQ51_19575"
     CDS             354..824
                     /locus_tag="VGQ51_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV9038851.1"
                     /translation="MRNKPTPLLFVVLLAACSGNQPSTPNSGASSSLRINGTWRLLSG
                     TTITKGVSSVTDYTRDQSMIKIINDTHFAFLKHRLNEPKDSSNQFDAGGGSYTLNGDQ
                     YTEHLDYYVDKNWEGKPFTFTVSIDGDTLVQKGLEKVESENIDRVIIEKYLRIK"
     gene            926..999
                     /locus_tag="VGQ51_19580"
     tRNA            926..999
                     /locus_tag="VGQ51_19580"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Met,seq:cat)
     gene            1115..2320
                     /locus_tag="VGQ51_19585"
     CDS             1115..2320
                     /locus_tag="VGQ51_19585"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV9038852.1"
                     /translation="MCPSLIRPVAYPLLFLLMVYQRVQGQNLVPNPSFEDVNICAEFG
                     QPCSPSAWFYLSRKFTTGYFPRYPSATGSRHLQIVAASRETVNRQYWETMLLCPLLAG
                     ERYSVSMKIASPVNRTDLGKRCPNLRDIGLWFTTRFIFVDGDSILQPPTYLSLNNASA
                     RDLENGWFEIKKEFVATANATILIVGNFERAQNVDLMDLRNPTTPTLDVLVDDLSLVR
                     VKGGSCAGSKKLKDWLYSVTERHSRKLSGDLVPTRADTLPDNPAKDQPGSGSEVDTLK
                     VTHPQLGTDTPKVANPPAQTDTIIVHNIQFDFDKYLVENPDTLEQYRRLLTRPGIRRI
                     QVVGYTDDAGSDAYNLDLSQKRAKEVAGLLASKFGIDPELIETEGRGISRKYPTKFLN
                     RRVEIYIFH"
     gene            2380..>4559
                     /locus_tag="VGQ51_19590"
     CDS             2380..>4559
                     /locus_tag="VGQ51_19590"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV9038853.1"
                     /translation="MLKNYLKTALRSLRKNGIYSVINIGGLSVSLAASILLLLWARDE
                     LSYDRFNIHAKQLYRLLPSVDNAGTANVWDYTSAPIAVFAKNEVPEVADACRMMDNWG
                     VSFFEFGGKKITEWHNKFVDPAFFSMFTFPLVKGDARRPFADAYSVILSETTARRFFG
                     DMDPMGKVMEGDDRKIYHVTGVMKDIPENSSIQFNIAFNFGQLAKEFDTTHYWKSLNS
                     AWGQYNYDTYLQLRGGANPGVVAEKLTAIHRSNERDNFTRHLTYRLNPMTQTHLYTSD
                     GREQGMLIVRIFLLAAMIVLVIACINYVNLVTARAIRRSKEISLRKIVGAGRAGLFMQ
                     LVSESVVLFLISLVLATGIIYLLMPLYNHLTDKNMVFRPWSPEVLSVYGLALGATLLL
                     AGIYPAITLSSLRPLAAMKGKLSGIGTRGGFRKVLVVVQFGFSILLITGTFIIGRQLN
                     YIRRMNLGYNKENVFSFWMRNIDKHYGAVKAELEAQPGILGVTESGVDIINNHTGTSD
                     ASWDGKRADQQAFTIGQMPVENNFTKVMGISLIEGTGFAGTPSDSMRFILNETAVKAA
                     GIPEPVVGRRFTLHGVKGVIAGVVKDFHFQNMRTVIHPLVIHYSKDWRGKMYVRTTGR
                     NASGALAAVERIWSRYNAGYDFSYDFLDSEFNDLYKTDRHVGQLFNCFAVLAILISCL
                     GLFGLVTYTAESRLREISVRKVLGAGVPHIVFLLSKDFMKLVVIA"
BASE COUNT         1068 a         1132 c         1251 g         1108 t
ORIGIN      
        1 gggtgatccg gaggtgaaag tcataattcc ccttcagcaa tcggaagaaa tggtcccggg
       61 tgatgataga gatctcgcag ggggtttgag caatggcggc agagatataa gggacgtcgt
      121 cgatcagcgc cgcgacaccg aagcaatgtc cggctttgta aagaccctga atgaattctt
      181 ttccttcgct attactgttc acgatggcta tttcgccgtc ctttacctca taaaaataac
      241 gggcatgggc atcctgccga tagatcagat cccctttttg gaaggttttg acggggacgg
      301 ctccttcgat aaaatcttgc agaagcatga tcgaaactac gtatttttaa aatatgagaa
      361 acaaaccaac ccccttgttg ttcgtcgtct tgctggcggc ctgtagcggc aatcaaccct
      421 cgacccccaa tagcggcgcc tcttcctccc tccggatcaa tggcacatgg cgactgttgt
      481 ccggcacgac catcacgaaa ggcgtgtctt cggtcaccga ctataccagg gatcagagca
      541 tgatcaagat catcaatgac acccattttg cctttctgaa acacaggttg aatgagccga
      601 aggactcttc caatcaattc gatgccggcg gcggaagcta tacgctgaat ggcgatcagt
      661 ataccgagca cctggattat tatgtagaca agaactggga agggaaacct tttaccttta
      721 cggtctccat cgatggggat acccttgttc agaaaggcct ggaaaaggtg gagagcgaga
      781 acatcgaccg cgtgatcatc gagaagtacc tgcggatcaa atagtaacgc gtagcttgca
      841 ttttttcggg gattttatct atttttgcca cccgtttggg gtcggtgcgt gcattttcgc
      901 gcttcgccag gcccgctgat attttgccga gatagctcag atggttagag cgtcggattc
      961 ataacccgga ggtctcgggt tcaactcccg atctcggtac tttaggtgta aatcccggga
     1021 agagctacga ttggtagctc tttttctttt taggagcgta tggtgaaaag ggttaaaatt
     1081 tcataaattg ggtccaacat ctctattcgc tcaaatgtgc ccatccctta ttcgaccagt
     1141 agcctatccg ctccttttcc tgctcatggt ataccaaagg gttcagggtc agaatcttgt
     1201 gcccaacccc tccttcgaag acgtcaatat ttgcgcagaa ttcggtcagc cttgcagtcc
     1261 ttcggcatgg ttctatctga gccggaaatt cacgacgggg tactttccgc ggtatcccag
     1321 cgccaccggg agccggcact tacaaatcgt cgcggccagc cgggaaaccg tcaaccggca
     1381 gtattgggaa accatgcttt tgtgtccgtt gctggccggt gagcgatatt cggtaagtat
     1441 gaaaattgca agtccggtca accggaccga cctgggtaag cggtgcccta acctgcgtga
     1501 cattggtctg tggtttacga cccggttcat attcgtggat ggcgactcca tcctgcaacc
     1561 cccaacctat ctttccctca acaatgcatc ggcccgggat ctggagaatg gctggttcga
     1621 gataaagaaa gaatttgtgg caacggccaa tgccaccatt ctgatcgtcg gcaattttga
     1681 gcgggcgcaa aatgtcgacc tcatggatct gcggaatccc accaccccga cgctggatgt
     1741 cctggtagac gatctctcgc tggtgcgcgt aaagggaggc tcctgtgccg gttctaagaa
     1801 attaaaagac tggttgtata gcgtcactga gcggcattcc agaaagcttt ccggtgatct
     1861 ggtccccacc agggccgata ccttgccgga taaccctgca aaggaccaac cggggtccgg
     1921 gtcggaggtc gatacgctga aggtgaccca tccgcaattg ggtaccgaca cgccgaaagt
     1981 agccaaccca ccagcgcaga ccgacacgat catagtccac aatatccagt tcgatttcga
     2041 taaatacctt gtcgaaaatc cggataccct ggagcaatac cggcgcctgc tgaccaggcc
     2101 gggtattagg aggatacagg ttgttggtta tacggatgac gccggttccg atgcttataa
     2161 cctcgatctt tcacaaaaaa gagcaaagga ggtcgccggt ctgcttgcgt cgaaattcgg
     2221 cattgacccg gagctcatag agacggaggg ccgggggata tctcgcaaat acccgacgaa
     2281 gttcctgaac cggcgcgtgg agatctatat ctttcattga cgcgcggttt ttgtaacctt
     2341 tttgagtttc cggcagtatt catagaataa gccatcccca tgctcaaaaa ttatctcaaa
     2401 accgccttgc gcagcctccg caaaaacggg atctattcgg tgatcaatat cgggggactg
     2461 tcggtgagct tggccgccag catattgttg ctgctctggg cgcgggacga gttgagttac
     2521 gatagattca acatccatgc aaaacaactt taccggctgc tgcccagcgt cgacaacgcg
     2581 gggacggcca atgtttggga ctatacgtct gcacccatcg ccgtatttgc aaaaaatgag
     2641 gtgccggaag tggccgatgc ctgccggatg atggataatt ggggcgtatc gttcttcgaa
     2701 ttcgggggaa agaagatcac ggaatggcat aataagttcg tcgaccccgc tttcttcagc
     2761 atgttcacct ttccgctggt gaaaggcgat gcgcgacgcc ctttcgcgga tgcctattcc
     2821 gtcatccttt ctgaaacgac ggccagacgt tttttcggtg atatggaccc tatgggtaaa
     2881 gtgatggagg gagacgacag gaagatctat cacgtcacgg gcgtcatgaa ggatatcccg
     2941 gagaattcct ccattcagtt caatatcgcg ttcaatttcg ggcagctggc caaagaattc
     3001 gataccacgc attactggaa aagcctgaac agcgcctggg gacaatataa ttacgatacc
     3061 tatctgcaat tgcgtggagg cgccaatccc ggtgtcgtgg ccgaaaagtt gacagcgata
     3121 catcgcagca acgagcggga caattttacc aggcatctca catatcggct gaacccgatg
     3181 acccaaacac acttgtatac ttcggacggc agagagcagg gtatgctgat cgtgcggata
     3241 tttctgttgg ccgcaatgat cgtgctcgtc attgcgtgca tcaattatgt caacctggtg
     3301 acggccaggg ccatccggcg gagcaaggaa atcagcttgc gcaagatagt gggcgccggc
     3361 agggcaggcc tttttatgca gctggtgagt gaatcggtgg tcctgttcct gatctcgctg
     3421 gtattggcta ccggcattat ctacctgtta atgcccctgt acaaccattt aacggacaag
     3481 aacatggtat tccggccctg gtcgccggaa gtgctgtctg tttatggtct tgcattgggg
     3541 gcgacgctat tgctagccgg catttatccg gctattacgc tgtcttcttt acggccgctg
     3601 gcggccatga agggaaagct cagcggtatc ggaaccaggg gtggttttcg aaaagtgttg
     3661 gtcgtggtgc agtttgggtt ttcgatcctg ctgatcacgg gcactttcat catcggccgg
     3721 cagctgaact acattcgccg gatgaacctg ggttataaca aggagaatgt cttcagcttc
     3781 tggatgcgca atatcgataa gcattatggc gcggtgaagg ctgaattgga ggctcagccg
     3841 ggcattttag gcgttacgga atcgggagtg gatatcatca acaaccatac gggtacctcc
     3901 gatgccagct gggatgggaa gcgggcggac cagcaggcct ttaccatcgg ccagatgccg
     3961 gtggagaata attttacgaa ggtgatggga atatccctta tcgaagggac ggggtttgcc
     4021 ggcacaccgt cggacagcat gcgttttatc ctgaatgaaa ccgcggtcaa agccgcgggc
     4081 atccctgaac ctgtcgtagg ccgacggttt acgctgcatg gcgtcaaagg cgtgatcgcg
     4141 ggcgtagtaa aggatttcca ttttcagaat atgcgcaccg tgatacaccc gctggtgatc
     4201 cattacagca aggactggcg ggggaagatg tatgtccgga ccaccggcag gaatgcctcg
     4261 ggggcgctgg ccgccgtaga aaggatatgg agccggtaca atgccggtta cgacttcagt
     4321 tatgattttt tagacagcga attcaatgac ctgtataaaa ccgaccggca tgtcgggcaa
     4381 ttgttcaatt gttttgcagt gctggcaata ttgatttcgt gtctggggtt atttggcctt
     4441 gtaacatata ccgcggaaag ccggctccgc gagatcagcg tacgcaaggt gcttggcgcg
     4501 ggtgtgccgc acatcgtttt tttattgtcg aaggatttta tgaagttggt ggtcatcgc
//
